LOCUS       DAGSRM010000001       111734 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.001, whole genome shotgun sequence.
ACCESSION   DAGSRM010000001 DAGSRM010000000
VERSION     DAGSRM010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 111734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 111734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..111734
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.001"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            207..1400
                     /locus_tag="MJE33_000001"
     CDS             207..1400
                     /locus_tag="MJE33_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HBY9645987.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDAEVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
     gene            complement(1612..2694)
                     /locus_tag="MJE33_000002"
     CDS             complement(1612..2694)
                     /locus_tag="MJE33_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBY9645988.1"
                     /translation="MKKIRKLATKLLIAMMVLTSCMSVTVYGMTAKDVTAKTPKSYVA
                     GTNSIYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKIRVAYNHIKNNTTYVNWNA
                     AEGANTAYTLVTKKGACSGMCRSLKALCDAMGIECYYVHSTTNDHQWNLIRFEDGRLY
                     HVDIDANKSAGADIIYKSLTHPLPFDKKAYPAVGEVVKTQNPTPTVKKPTPAKPSAPV
                     GPGNHTMIADQQGAILDEVYGNNLRPKIEMYEGVPCSLINSSINTIKFTSKNSNLDPS
                     KHTVTVIGFMNTPYGMVALAPDGVPDEYPFEYDKPFSLDFTGKNLSAAELSKKCKENN
                     VIIKVEIGETASNSGRGMVAYARYVN"
     gene            complement(3258..4049)
                     /locus_tag="MJE33_000003"
     CDS             complement(3258..4049)
                     /locus_tag="MJE33_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9645989.1"
                     /translation="MKLIDEYLDKLYKKCDNKSTIELKQEMRCHLIESANEFKLEGLD
                     EEEACKKAIERFDDGDEMQYELCNIIKELSLSLDRHKSIVMGFKKVLGYISIIAFLIS
                     GFMWYYNNSLQHNMYNLGKELDGEIKQLAERHDMTKIGEYKLELEKILDKDKYSKVKA
                     LRLYVIDMKDGNTNLSSSGLNANMVYEREADYNNISNFIQHLGYNGKDFLDKNGNIVN
                     PDIFLEYFFYFESEMLIPVAFAFGLLCIIAYFILRFKISLIKNNN"
     gene            complement(4053..4382)
                     /locus_tag="MJE33_000004"
     CDS             complement(4053..4382)
                     /locus_tag="MJE33_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY9645990.1"
                     /translation="MQLNKEVLKGYIDILIVSILEKKDCYGYEIAKQVRERSEFELKE
                     GTMYLALKRMESKNLIKSYYSNEQSSGGRRKYYNLTNEGKDFLEIKKQEWRFIKKVMN
                     QFLGEVD"
     gene            complement(4867..5280)
                     /locus_tag="MJE33_000005"
     CDS             complement(4867..5280)
                     /locus_tag="MJE33_000005"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBY9645991.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRI"
     gene            complement(5321..6451)
                     /gene="rodA"
                     /locus_tag="MJE33_000006"
     CDS             complement(5321..6451)
                     /gene="rodA"
                     /locus_tag="MJE33_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HBY9645992.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNLLGRYYKALYIISLILLAIVLLPGIGT
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVVVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKVR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQEDFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            complement(6536..6820)
                     /gene="minE"
                     /locus_tag="MJE33_000007"
     CDS             complement(6536..6820)
                     /gene="minE"
                     /locus_tag="MJE33_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HBY9645993.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            complement(6838..7635)
                     /gene="minD"
                     /locus_tag="MJE33_000008"
     CDS             complement(6838..7635)
                     /gene="minD"
                     /locus_tag="MJE33_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HBY9645994.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            complement(7659..8342)
                     /locus_tag="MJE33_000009"
     CDS             complement(7659..8342)
                     /locus_tag="MJE33_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HBY9645995.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYESESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            complement(8440..11418)
                     /locus_tag="MJE33_000010"
     CDS             complement(8440..11418)
                     /locus_tag="MJE33_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBY9645996.1"
                     /translation="MEENKQVDRLKIIKIVMGVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEESAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMLTSMAALENGLD
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPD
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKLDVKEDRIEEV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRYVAAIANGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVARQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            complement(11430..11918)
                     /gene="mreD"
                     /locus_tag="MJE33_000011"
     CDS             complement(11430..11918)
                     /gene="mreD"
                     /locus_tag="MJE33_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBY9645997.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAAIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSYNISTLFVKGLLVAPIVNSLIGLVIYRLSKRAVLKLK
                     ED"
     gene            complement(11933..12826)
                     /gene="mreC"
                     /locus_tag="MJE33_000012"
     CDS             complement(11933..12826)
                     /gene="mreC"
                     /locus_tag="MJE33_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBY9645998.1"
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGS
                     PVNLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKNMIA
                     LNAKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGVKK
                     ESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKD
                     SYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVV
                     KPYVDFKNINDVVVIEPRNIG"
     gene            complement(12836..13846)
                     /locus_tag="MJE33_000013"
     CDS             complement(12836..13846)
                     /locus_tag="MJE33_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBY9645999.1"
                     /translation="MTKDMGIDLGTANTLVYIKGQGIVVREPSVVAIRDDSKEVLAVG
                     EEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQKAADKKGVVSPRIAICVP
                     FGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVEEPEGNMVVDIGGGTSEI
                     AVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERTAENVKINIGSTFKDDEE
                     INMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIKSTLERTPPELASDIMEN
                     GIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTGKSVEDQEIFEKVLMMNT
                     KN"
     gene            complement(13967..14626)
                     /gene="radC"
                     /locus_tag="MJE33_000014"
     CDS             complement(13967..14626)
                     /gene="radC"
                     /locus_tag="MJE33_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBY9646000.1"
                     /translation="MAPEERPREKMLAKGVKSLSNAELLAILLRTGNKNKNAIELANY
                     IINRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGSRVASFKPIKYKIRNPW
                     DIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSSSLVHPREVFKEAIRRS
                     ASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGDGLYFSFKENMI
                     I"
     gene            complement(14671..15255)
                     /gene="maf"
                     /locus_tag="MJE33_000015"
     CDS             complement(14671..15255)
                     /gene="maf"
                     /locus_tag="MJE33_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBY9646001.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     gene            complement(15475..16446)
                     /locus_tag="MJE33_000016"
     CDS             complement(15475..16446)
                     /locus_tag="MJE33_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HBY9646002.1"
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLP
                     GANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKV
                     ICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDG
                     IAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIGCG
                     MCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQELCV
                     GCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            complement(16462..17037)
                     /gene="rsxA"
                     /locus_tag="MJE33_000017"
     CDS             complement(16462..17037)
                     /gene="rsxA"
                     /locus_tag="MJE33_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HBY9646003.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            complement(17052..17645)
                     /locus_tag="MJE33_000018"
     CDS             complement(17052..17645)
                     /locus_tag="MJE33_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HBY9646004.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            complement(17658..18227)
                     /locus_tag="MJE33_000019"
     CDS             complement(17658..18227)
                     /locus_tag="MJE33_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HBY9646005.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQANNEL
                     RKAVLPEAKEFKEMKEDTYKGLGDGLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            complement(18227..19204)
                     /locus_tag="MJE33_000020"
     CDS             complement(18227..19204)
                     /locus_tag="MJE33_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HBY9646006.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADLSSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            complement(19227..20564)
                     /gene="rsxC"
                     /locus_tag="MJE33_000021"
     CDS             complement(19227..20564)
                     /gene="rsxC"
                     /locus_tag="MJE33_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HBY9646007.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            complement(20780..21724)
                     /locus_tag="MJE33_000022"
     CDS             complement(20780..21724)
                     /locus_tag="MJE33_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646008.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            complement(21835..23109)
                     /locus_tag="MJE33_000023"
     CDS             complement(21835..23109)
                     /locus_tag="MJE33_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBY9646009.1"
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSD
                     CDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSSQNNPTSS
                     GTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDIVKLLEQSNGWYKIKLSNGVTG
                     WASSQYISKTSEDVGTNNSSNSNSTNNSDKKPSSEESIEGKNGKVTSAVSLNVRSGPG
                     TSYSIIGKLNGGDVVELKAKSNGWYKVKLSSGTIGWVSASYISETNEGTKEKPNSSSN
                     QNSQPNSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYV
                     FKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIH
                     CSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            complement(23241..23612)
                     /locus_tag="MJE33_000024"
     CDS             complement(23241..23612)
                     /locus_tag="MJE33_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBY9646010.1"
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNH"
     gene            complement(23840..24439)
                     /locus_tag="MJE33_000025"
     CDS             complement(23840..24439)
                     /locus_tag="MJE33_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646011.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
     gene            complement(24640..24852)
                     /locus_tag="MJE33_000026"
     CDS             complement(24640..24852)
                     /locus_tag="MJE33_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HBY9646012.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDDVSIEKLKEVIAELGFELKGIE"
     gene            complement(24925..26640)
                     /locus_tag="MJE33_000027"
     CDS             complement(24925..26640)
                     /locus_tag="MJE33_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBY9646013.1"
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKNVTSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEDARTSMVMALDSDITNVTLNDLSKFQEKEFNITKYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKAIECLAKVEYANVNSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKAKKAEE
                     DRIKKEAEEKKNREKEEVKKSLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNATFISLAVRNNYIVSNSRLKGKITPNDFDVYEGISNLDIK
                     SNESN"
     gene            complement(26640..27194)
                     /locus_tag="MJE33_000028"
     CDS             complement(26640..27194)
                     /locus_tag="MJE33_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBY9646014.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEHYNKIYGKKGKN"
     gene            complement(27187..27789)
                     /locus_tag="MJE33_000029"
     CDS             complement(27187..27789)
                     /locus_tag="MJE33_000029"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBY9646015.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNIPIVDADIISRKIFEDKLL
                     LEKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNNLTHPRIREKIISEIEKLR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSEQEALSRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            complement(27798..30446)
                     /gene="polA"
                     /locus_tag="MJE33_000030"
     CDS             complement(27798..30446)
                     /gene="polA"
                     /locus_tag="MJE33_000030"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBY9646016.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKDYKAGRKGMPDELAEQLQPLKDLLDKFRINR
                     LEIDGYEADDIIGTVSKKAEENGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPTQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMIFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSDDTEHVEEKIEILKLKDIEKFIDEVNKKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITIAEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETVPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNMDVHLDVDLNVGSSWYETK"
     gene            30598..30685
                     /locus_tag="MJE33_000031"
     tRNA            30598..30685
                     /locus_tag="MJE33_000031"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30632..30634,aa:Leu,seq:caa)
     gene            complement(30872..31315)
                     /locus_tag="MJE33_000032"
     CDS             complement(30872..31315)
                     /locus_tag="MJE33_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646017.1"
                     /translation="MKKNMLYVGIIYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GVCMILQYMYWSKPERAVDYEEKIKNQRIEMNDERRIMLRDKAGRITNQIMCYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKLDKRL"
     gene            complement(31318..31515)
                     /locus_tag="MJE33_000033"
     CDS             complement(31318..31515)
                     /locus_tag="MJE33_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY9646018.1"
                     /translation="MKNRLEEIRKERGIKQEELASILQVSRQTIGSLENGRYNPSIIL
                     AFKIARYFDMSIEEIFIYEEE"
     gene            complement(31707..32573)
                     /locus_tag="MJE33_000034"
     CDS             complement(31707..32573)
                     /locus_tag="MJE33_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBY9646019.1"
                     /translation="MEWINKLNQAITYIEENLESGVDYAEAAKIACCSTFHFQRMFSY
                     IAEVPLSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNIEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPVLEDTHEYLI
                     PSGTWAVFECVSPMPNALAIQELQKRIITEWLPSSGYVYANLPDIELYPNGDINAPDY
                     TTEVWIPIQKPS"
     gene            complement(32799..33668)
                     /locus_tag="MJE33_000035"
     CDS             complement(32799..33668)
                     /locus_tag="MJE33_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBY9646020.1"
                     /translation="MDYKTIILNRKSVREYKNTDIKEEYLSEIKKYSESCKKLVPEID
                     VDVRIMNRSEVFNNLDKVAGYKGNMIDSPSYIIILSDIKDNYIENSGYIGESIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGINSDKEVTGIIAIGYGENVSNAKVLNATKTGQNYSK
                     SDMQVVTDKGSTRLGVEDVVYMNEWGNVASIEILEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVVLAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFDLKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
     gene            complement(34174..34395)
                     /locus_tag="MJE33_000036"
     CDS             complement(34174..34395)
                     /locus_tag="MJE33_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646021.1"
                     /translation="MNVNNHKCPECGEHSKKFEIVEENEDCVIFECYTCGTISILKYC
                     ENCKEFVFVNIPNNVETYEYCCGKCNNKI"
     gene            complement(34897..35877)
                     /locus_tag="MJE33_000037"
     CDS             complement(34897..35877)
                     /locus_tag="MJE33_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646022.1"
                     /translation="MGDTSRFYESVKNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDSLSWKQIVILAECDGKNTTDDIINILSNRYRNIHNMKEKVMEFFMFYENV
                     YLTFEDDIQTLSSVFEITGNKSYITPLYFTIEIDGDNNTNDYFSEITSLLNCMYEKGC
                     RFIEVIGEDILKNKSMREIFQYMLDHFDLIVVTKDSFTIDRSLIKELDNYRHKVIWKV
                     YSKNDDTRIKLKEDIKITSLIKRGHTVVRGDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSDGSVKSYSFQKDKRIYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
     gene            complement(36041..36913)
                     /locus_tag="MJE33_000038"
     CDS             complement(36041..36913)
                     /locus_tag="MJE33_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBY9646023.1"
                     /translation="MIIKVSMTVLVLILLYILYKYETQHIETTEYTIFNKKIPKEFDN
                     FKIVQVSDLHNKVFDKNNKVLIDKIESLNPDVIFITGDLVDGENKNFQVALDLIDNLV
                     EKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYSKKIINMENESVRIYRGNSHLNLYG
                     LIIPLECYPYLFANNKKLELSDNFVEDNLGKVNENEYNILLAHTPFFFEKYAKWGADL
                     VLAGHVHGGIIRIPFVGGVLSPNREFFPKYDWGKYEKDNSTMILNKGLGGSKVLIRLN
                     CKPEIVKITFKYEK"
     gene            complement(37077..37628)
                     /locus_tag="MJE33_000039"
     CDS             complement(37077..37628)
                     /locus_tag="MJE33_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY9646024.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQKGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSHVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHIYKNLNKGKTIFHNIEVYR"
     gene            complement(37648..38550)
                     /locus_tag="MJE33_000040"
     CDS             complement(37648..38550)
                     /locus_tag="MJE33_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBY9646025.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCVSCNRCIDVCPQNISSLFDRAQINNKCDLCGECIKVCLKDAICIMT
                     EEMSVEEIVEEVEKDFIFFFESNGGITFSGGEPTLQIDFLRELVDIFYDKGINIAIET
                     CGYFDWNKVNDVFEKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRNTALFVKQNVPGGKMELLPYHKFGIDKYKALGLEDYIYEFDE
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            complement(38565..40937)
                     /locus_tag="MJE33_000041"
     CDS             complement(38565..40937)
                     /locus_tag="MJE33_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HBY9646026.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDKRTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAQGIMTLAQR
                     YSNLAKEMSKQETDEKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDTKNITEFTSFEELKDEFLKQLAYLVKSSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMIKSYFALGGFHVQFNT
                     ISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
     gene            complement(41158..41847)
                     /locus_tag="MJE33_000042"
     CDS             complement(41158..41847)
                     /locus_tag="MJE33_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBY9646027.1"
                     /translation="MKVKRNINRVAIIVALNLSLALMVGCMSPVSDKNLSSSEQISKD
                     SLKEQVEDKKDESIELFNNYSQKINDAKGREEVKKDLSQRFSKAIIDSGYSITNSKSD
                     GSFVVSINGAEKPPEKNIRSLFYSMSEDDKEGKVTIDILCAKEYPEDDKLSESDKYVK
                     FIYNLFKSLTDTNLTEKEIFSMVEEDFNKGVGVVELPYMKDIHVEVNKVKQSTKVLKL
                     SLVYKFDISNH"
     gene            complement(42088..43467)
                     /locus_tag="MJE33_000043"
     CDS             complement(42088..43467)
                     /locus_tag="MJE33_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBY9646028.1"
                     /translation="MLNFMTELLVVLSIFIQFSIMNGILIYKKDSKKNEKIVFYIVLF
                     LIYIIIQISRLFNGGNYFSNINYLFYILAFIAWGGYYNYFYKMGYIKYSIFFYLFSVY
                     YKCLDAVIYRILFFAITGNVISSKESLLVPYLESIKIQIYSNLIIILIYLIFSFFRKS
                     IKLISQDKRNYVYLLFALLVNTMNMLVNYVLKKLERFGSLHSEGYYFDNFVNPKLVGA
                     SSIFLILLFKEIIKENRLKSQAELIKNKLDMQYAHYLSIQESHMRVKKLYHDINNHIY
                     CIDNLRNNSKEINEYVNNLKDEIKTFKYIYNTGNMILDIIINEKSEVCLKKGIKFTCS
                     INFSKVNFVKPIDVSSIFSNILDNAIEACDKIVDENINKYIRIKGTITRSFFVLKCEN
                     SKLNQLTFNKNILLTNKMDKFVHGIGIQSIKSSLQKYNGELLFENSIDKFILNIYIPL
                     DQDTDSWGD"
     gene            complement(43461..44174)
                     /gene="rgbR"
                     /locus_tag="MJE33_000044"
     CDS             complement(43461..44174)
                     /gene="rgbR"
                     /locus_tag="MJE33_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgbR"
                     /protein_id="HBY9646029.1"
                     /translation="MIKIAVCEDEKETQLLIEDYLENILKDISIEYEIQKYISGEELL
                     ESNLKDIDILLLDIKMEKLNGMDTARKIREVDNEMEIIFVTSLIDYVQEGYEVRAYRY
                     LLKPIELEELKKHVLTCIKDIEINKESHITIKNKSNTYKIYLNEIKYIEVQKKDMLIH
                     TINKNFDIKYSLGKIEKELNPYKFIRCHKSFIVNLRYVENIKPNTAILESGEEVPISR
                     YRYKEVKEKFLKFLGDTIC"
     gene            complement(44424..44984)
                     /locus_tag="MJE33_000045"
     CDS             complement(44424..44984)
                     /locus_tag="MJE33_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HBY9646030.1"
                     /translation="MSAMEKKIKFKFDWYSSPDKIEKWLEDIEAKGYNLYKINKFGGI
                     FYFHKGSPRKIKYTVDYYYDVENNYFDEHKSNGWNLVFRSIGSFMGTSAWYIWSKEYK
                     DIPPKLYSDSDKTNKVKSILFMNMFIFFMLVVIFIYLLNIEIRNLYSGYGASVTAIVL
                     QIILLILYAVQLYRRLMYYLRLKKFK"
     gene            complement(45079..45882)
                     /gene="zupT"
                     /locus_tag="MJE33_000046"
     CDS             complement(45079..45882)
                     /gene="zupT"
                     /locus_tag="MJE33_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HBY9646031.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGSWSTVIAFFIGMAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGIFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYVLLRNFLNDITLGI
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            complement(46033..47196)
                     /locus_tag="MJE33_000047"
     CDS             complement(46033..47196)
                     /locus_tag="MJE33_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBY9646032.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     IGIPYVSAGGTGVIATIKGANPGKTVALRGDMDALQVVECTDVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAILEGTIRLFNPELRKQIPRILERIAKS
                     TAEAYRADAELEYGYLTPAVINDKECSKIATDAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDALEIGTALYVQYAVDFLNK"
     gene            complement(47256..48443)
                     /locus_tag="MJE33_000048"
     CDS             complement(47256..48443)
                     /locus_tag="MJE33_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646033.1"
                     /translation="MEAITAFCLVLVSLTIGDIVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTVVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTDEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVICLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIVGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            complement(48806..50506)
                     /locus_tag="MJE33_000049"
     CDS             complement(48806..50506)
                     /locus_tag="MJE33_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9646034.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIKDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPDGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDKGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            complement(50761..51036)
                     /locus_tag="MJE33_000050"
     CDS             complement(50761..51036)
                     /locus_tag="MJE33_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY9646035.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIQAEVEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
     gene            complement(51155..51727)
                     /gene="mobB"
                     /locus_tag="MJE33_000051"
     CDS             complement(51155..51727)
                     /gene="mobB"
                     /locus_tag="MJE33_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HBY9646036.1"
                     /translation="MLLLDSFMKGHNFMYNILSVVSYSGVGKTTLIEGIIKELNKKGY
                     RVGVIKHTCHDFDMDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKEKNPLYKMIQL
                     YEDMDLIIIEGYKNYRFKRLEVTRKGKYEDILSNKSDLIGIVSDIEYKLNIEQFNLND
                     YKNISKYIESCIKNEILSISNVEIKNILKE"
     gene            complement(51711..52892)
                     /locus_tag="MJE33_000052"
     CDS             complement(51711..52892)
                     /locus_tag="MJE33_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HBY9646037.1"
                     /translation="MIELEDAIKIIEKNVLPINRVKIVNLIDAVNKVVAEDIYSTIDS
                     PPFDRSPYDGYAYNANSENRNLKVIGTLYAGDVFNRVLNKNEAVKIMTGGKIPQGANC
                     VIKKEDVTVIGDMITLNIKLKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASLG
                     ISKVTVYDDLKVGILNTGTELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNC
                     SDDILDLEKKVKNMLQSVDFLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGA
                     CYVGTLGEKLLFGLSGNPHAAIVVFDNVIVPVIEYLTYKKGTHHIKAIFKGEFNKLSN
                     KDRFVSGKLYTSEGKLYVELNDKKDAKARLSATLRSNCMIKIKKNETIKENQLVDVVI
                     R"
     gene            complement(53103..53837)
                     /locus_tag="MJE33_000053"
     CDS             complement(53103..53837)
                     /locus_tag="MJE33_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646038.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTKEKNQVASKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINENKVVNVETNSFSSQGISVIDAESSIVIEKSDHEKSAVSLENFR
                     HIDLGEYVGVEDSRINEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESDKIVKIIKGTLLE"
     gene            54199..55104
                     /locus_tag="MJE33_000054"
     CDS             54199..55104
                     /locus_tag="MJE33_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBY9646039.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFMKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVSK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKDVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
     gene            complement(55264..56106)
                     /locus_tag="MJE33_000055"
     CDS             complement(55264..56106)
                     /locus_tag="MJE33_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBY9646040.1"
                     /translation="MTTSSKKLETISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSGSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGVV"
     gene            complement(56090..56848)
                     /locus_tag="MJE33_000056"
     CDS             complement(56090..56848)
                     /locus_tag="MJE33_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBY9646041.1"
                     /translation="MEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALGNL
                     HTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPIAT
                     LVLLIDNFLMTVVLTWGAHIADRYAEEGNIEGVERVQLLFGIGNKLILAIIVGIGFSL
                     GVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVAYL
                     NITVTGVALFGLAIALIYVNFVGEKEVIVDDNEF"
     gene            complement(56865..57335)
                     /locus_tag="MJE33_000057"
     CDS             complement(56865..57335)
                     /locus_tag="MJE33_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY9646042.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDDLLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            complement(57335..58078)
                     /locus_tag="MJE33_000058"
     CDS             complement(57335..58078)
                     /locus_tag="MJE33_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane biogenesis protein"
                     /protein_id="HBY9646043.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEITIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKANLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENDVEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLKLGDMAIIGSYF
                     KDNYKDFGDVSVEHVKTFMDEIKKIREEL"
     gene            complement(58082..58495)
                     /locus_tag="MJE33_000059"
     CDS             complement(58082..58495)
                     /locus_tag="MJE33_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY9646044.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDKECEYIILSDLYGASVCTAMLRLTNLHNVRLFSGMNLNMVLELLTR
                     FSDELTVEDIDQLVEESRRGIQSVTLQIKEEEEVF"
     gene            complement(58521..59273)
                     /locus_tag="MJE33_000060"
     CDS             complement(58521..59273)
                     /locus_tag="MJE33_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY9646045.1"
                     /translation="MDEKRQKKEMDLEVNSFVPLYQQLYDNIKKQIASGIYKPGDKLP
                     SEGDLCKEFNISRITVRNALNELVKEDILCKKRGKGTYVTIPERIEATCAGNSFTNSC
                     HRINAKPSTKIISVLIKKADKQVAEALSIELEEKVICIKRLRLIDEVPVIFEVDYFRI
                     DYMFLLKEELEDKSLMEVISNNISTLPKRVENIFEVKHSNKEYSDHLKCASNMPLLKV
                     RQSVYTENNDVLYYNEQFIRSDKYKYAVSAEI"
     gene            complement(59513..60907)
                     /locus_tag="MJE33_000061"
     CDS             complement(59513..60907)
                     /locus_tag="MJE33_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBY9646046.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSRNYMEFLSKSKTERLSV
                     KEIIKLAKENNYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTAIPLALHGIVILNDGTKVDIS
                     IGEEDSDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNIPLEDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGLDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKRI
                     FNKGNVVWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            complement(60927..61136)
                     /locus_tag="MJE33_000062"
     CDS             complement(60927..61136)
                     /locus_tag="MJE33_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HBY9646047.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            complement(61165..62610)
                     /locus_tag="MJE33_000063"
     CDS             complement(61165..62610)
                     /locus_tag="MJE33_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBY9646048.1"
                     /translation="MEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPIRKL
                     SNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSLNSYKSGIDNRKNKIIVQREDL
                     IHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSDGNI
                     KTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISIPML
                     DDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYIIEDD
                     FISDFVFESKDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLWAKY
                     SSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTDKYKLAKNLINDKLSGKLKVRKS
                     CGGINFFLELPRGYTSQAFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSMEEL
                     EKGISIISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            62720..63514
                     /locus_tag="MJE33_000064"
     CDS             62720..63514
                     /locus_tag="MJE33_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBY9646049.1"
                     /translation="MFYDYHMHSSFSTDGKSTMEQMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQSKYKDKISIKKGIEFGVQTQLMDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQHEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTVLDDRLFSDIIEETLKQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            63565..64887
                     /locus_tag="MJE33_000065"
     CDS             63565..64887
                     /locus_tag="MJE33_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBY9646050.1"
                     /translation="MKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLGIYH
                     MIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALIISIV
                     ASFNSSFLSLKFLKDAKLNLFILAVCPAIVIITLSNVLRGYYYGIKNVKIPAIGQIIE
                     QIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYIISI
                     KDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGIVSG
                     MVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSSIFF
                     YFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTITGM
                     LIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKSIR"
     gene            complement(64987..66204)
                     /gene="cdeC"
                     /locus_tag="MJE33_000066"
     CDS             complement(64987..66204)
                     /gene="cdeC"
                     /locus_tag="MJE33_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HBY9646051.1"
                     /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN
                     GCNTDNKCECHDDCNPCNPCNPCKPNPCNPCKPNPCDDNCGCHDNCKCDCEPCEMDSD
                     ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE
                     TEVVEVFGSVPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT
                     FEFAVCGERNSECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR
                     AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE
                     CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC
                     GPNHGSGKPSCHR"
     gene            complement(66387..66567)
                     /gene="ssrS"
                     /locus_tag="MJE33_000067"
     ncRNA           complement(66387..66567)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="MJE33_000067"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(66682..67587)
                     /locus_tag="MJE33_000068"
     CDS             complement(66682..67587)
                     /locus_tag="MJE33_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646052.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESVL
                     DLVRGIKKLNNIDMVDTEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDEDNN
                     DELNYEDEELDCEDKELDDDELTDEGEMEDNEDELVEDKNTGYENIENDEDIVYDEEL
                     YNINEQESEYNYNEKNTKTRAKKKR"
     gene            67799..68197
                     /locus_tag="MJE33_000069"
     CDS             67799..68197
                     /locus_tag="MJE33_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646053.1"
                     /translation="MKKLDTESLEKMAKQKNIDKDKIEKMADSYKGKSENELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDNIMEKISDAENQRDTKPAKTKKAKPTKGKS
                     NSPAPKNTPGSQQKKSKSLFKKTKKSNPHE"
     gene            complement(68320..69336)
                     /gene="ccpA"
                     /locus_tag="MJE33_000070"
     CDS             complement(68320..69336)
                     /gene="ccpA"
                     /locus_tag="MJE33_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator CcpA"
                     /protein_id="HBY9646054.1"
                     /translation="MKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEVIK
                     ETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANTYS
                     DKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYTVS
                     TSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKSLI
                     KYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVAGF
                     NDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRESVT
                     ERKK"
     gene            complement(69589..69789)
                     /locus_tag="MJE33_000071"
     CDS             complement(69589..69789)
                     /locus_tag="MJE33_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646055.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF
                     LILLLITCSCGGFFDGEVGCGC"
     gene            complement(69823..70002)
                     /locus_tag="MJE33_000072"
     CDS             complement(69823..70002)
                     /locus_tag="MJE33_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646056.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            complement(70184..70390)
                     /locus_tag="MJE33_000073"
     CDS             complement(70184..70390)
                     /locus_tag="MJE33_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646057.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            complement(70438..70863)
                     /locus_tag="MJE33_000074"
     CDS             complement(70438..70863)
                     /locus_tag="MJE33_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646058.1"
                     /translation="MKYVGLLLTSVGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            71035..71262
                     /gene="acpP"
                     /locus_tag="MJE33_000075"
     CDS             71035..71262
                     /gene="acpP"
                     /locus_tag="MJE33_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBY9646059.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            complement(71406..72410)
                     /locus_tag="MJE33_000076"
     CDS             complement(71406..72410)
                     /locus_tag="MJE33_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBY9646060.1"
                     /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK
                     DEDLRKGVFKDYNDNLIKNCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESCDLA
                     ICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGALIS
                     LSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRSIKTDNGFDIKGNLTDFEDPMKYTVKQNQDGTIDIIIKNVTFYEEK
                     "
     gene            complement(72424..73326)
                     /locus_tag="MJE33_000077"
     CDS             complement(72424..73326)
                     /locus_tag="MJE33_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBY9646061.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKREK
                     SVLVNGVYKKPSLKVYSGDLIEVKIDEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSVTHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGYVDEELINRQALHAYKLEFEQP
                     RTKEKLKFKADIPEDMKELISKLR"
     gene            complement(73521..74696)
                     /locus_tag="MJE33_000078"
     CDS             complement(73521..74696)
                     /locus_tag="MJE33_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBY9646062.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR"
     gene            complement(74783..75628)
                     /locus_tag="MJE33_000079"
     CDS             complement(74783..75628)
                     /locus_tag="MJE33_000079"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HBY9646063.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            complement(75726..76523)
                     /locus_tag="MJE33_000080"
     CDS             complement(75726..76523)
                     /locus_tag="MJE33_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBY9646064.1"
                     /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            complement(76542..77552)
                     /locus_tag="MJE33_000081"
     CDS             complement(76542..77552)
                     /locus_tag="MJE33_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBY9646065.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            complement(77571..78353)
                     /locus_tag="MJE33_000082"
     CDS             complement(77571..78353)
                     /locus_tag="MJE33_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBY9646066.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            complement(78412..79548)
                     /locus_tag="MJE33_000083"
     CDS             complement(78412..79548)
                     /locus_tag="MJE33_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HBY9646067.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLRPLASGEKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
     gene            complement(80257..80724)
                     /locus_tag="MJE33_000084"
     CDS             complement(80257..80724)
                     /locus_tag="MJE33_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HBY9646068.1"
                     /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG
                     LKNSIKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKCEKWVDVCKNW"
     gene            complement(80803..81345)
                     /locus_tag="MJE33_000085"
     CDS             complement(80803..81345)
                     /locus_tag="MJE33_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646069.1"
                     /translation="MKKFIKVSFFILGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV
                     FDFDSRRSRDVGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGMNKCSAYVNVDLF
                     NEKNSITERTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYIVNIPMA
                     KGLYLVVGKNNDTWNANIME"
     gene            complement(81397..82083)
                     /locus_tag="MJE33_000086"
     CDS             complement(81397..82083)
                     /locus_tag="MJE33_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBY9646070.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSL
                     LKMISMIVYQVKHFNYSKVGGVYLSNYVTDIANTKYINLYSMTFLTIIIFIISMYFSI
                     KISKKNKFNY"
     gene            complement(82083..82943)
                     /locus_tag="MJE33_000087"
     CDS             complement(82083..82943)
                     /locus_tag="MJE33_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9646071.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENSPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK
                     NFDRERFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI
                     VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVR"
     gene            83078..83878
                     /locus_tag="MJE33_000088"
     CDS             83078..83878
                     /locus_tag="MJE33_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HBY9646072.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            83903..84472
                     /gene="yfcE"
                     /locus_tag="MJE33_000089"
     CDS             83903..84472
                     /gene="yfcE"
                     /locus_tag="MJE33_000089"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HBY9646073.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YIESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
     gene            complement(84808..85860)
                     /locus_tag="MJE33_000090"
     CDS             complement(84808..85860)
                     /locus_tag="MJE33_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HBY9646074.1"
                     /translation="MKIKSKKIIAFALSLGIMLPSCVYADTISKNQNAQVDMNNYLQG
                     RKSPKEEKLIGEDRFDTAIKISQSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVTEEIKRLNPAEVILIGSEGAISSSVKESLEDMEVLVSRIGGADREE
                     TSLLLTRQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTKTYIVGGEYSISKKLEGQLVNSERLAGTDRKDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
     gene            complement(86086..87312)
                     /gene="pepT"
                     /locus_tag="MJE33_000091"
     CDS             complement(86086..87312)
                     /gene="pepT"
                     /locus_tag="MJE33_000091"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBY9646075.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGVDTIGFISHLDTIEDVSGKDIKPRIIENYDGKD
                     IVLNEALNVITYVKDSPELEEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFDVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIAAEISEMFPADERPETTEGYEGFWHLNSIGGNVENVS
                     MAYIIRDHCKEKFENRKSIMIENIEKINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELGIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            complement(87403..88455)
                     /gene="ytvI"
                     /locus_tag="MJE33_000092"
     CDS             complement(87403..88455)
                     /gene="ytvI"
                     /locus_tag="MJE33_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBY9646076.1"
                     /translation="MSILENNFLYKLKNNTIFFCIYTLLFIFIYKAFPYIAPFFLGAI
                     IALMINPISQKLENKFRINKGISTLVLSFLAVAIVSTVTTIIVINSMKELMGFLNNIS
                     ANPEDISNTIMYFLNKINDFMKSFQEIANFDVEQLVNKFSGEVMQITKNLLTSILGLA
                     TSIPYIIIFIITLFIATYFIAKDLDKIENSFYNMFTVDVRKKVKNVKKEAGLSLVGYI
                     RAYTILMAITFFAIWGSFALFGLKYGLIVGFVGALMDLIPFLGIITIYLPVIVYYFLI
                     KNYFIAISMTVIFFVLSLIREILEPKLVSVNVGLNPLATLAAIFIGIQVKGIIGVIFC
                     LGLVCMHDILKKVDIF"
     gene            complement(88604..89857)
                     /locus_tag="MJE33_000093"
     CDS             complement(88604..89857)
                     /locus_tag="MJE33_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBY9646077.1"
                     /translation="MLESPNNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIEKNRIKKIRIYT"
     gene            complement(89988..93515)
                     /locus_tag="MJE33_000094"
     CDS             complement(89988..93515)
                     /locus_tag="MJE33_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBY9646078.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNTQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNIQLQNLKDLKSKLSQYDGITEEVYNKIN
                     QELIELKNLEKDKNNALDLAQKSYEESKTVYEEQLKLEKNELRKNELDLKNSEIKEKI
                     ALVENDSNAKKVDPYISSVQNLEKKLDEDSFAVSNLEKKLAILNQELEVTKNRYEKIN
                     KIKNEEVPKLSEEKIRLQQAIKLEEELVLLDRELKELKESGINLNKTKVELEKVKQVS
                     ESRKDAVTKSIKEVEGKIDKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKLNKLEEI
                     LKETENINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRN
                     KANNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDDLEKELEELK
                     YLNLASELRRELKENMPCPVCGSKHHENHDITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKVSGYLSIEKMKTKELEDVKGKLGGIPSSQLLKKLDEEQRKLALLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKIKQ
                     EYLGLKTITKVSNLLSKVKEIRENEKELELLSTGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYVEKKLSRDNKYKEVISITKGDLAKNLLHNVEENICKILEQEESSKK
                     KLEEQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSTLNKLLAENKFESIYAVKRALLD
                     TDAVKKLIEEILEHEEEQKLLSFKIKSSKEKLNGRSIKKEYFEQLKDEIYNLKVEIGN
                     ISKDIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
     gene            complement(93502..94713)
                     /locus_tag="MJE33_000095"
     CDS             complement(93502..94713)
                     /locus_tag="MJE33_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBY9646079.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVVATLPYPSEKRLNEVFSSE
                     DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEINTDEIISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            complement(94759..98586)
                     /gene="addA"
                     /locus_tag="MJE33_000096"
     CDS             complement(94759..98586)
                     /gene="addA"
                     /locus_tag="MJE33_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBY9646080.1"
                     /translation="MSSPKWTKEQLEVIESRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRINLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSVEYKKIADISQACNKSWDEAYKK
                     MASMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISSVVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDVDEKGNIVP
                     SDIAVGYRNKFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFNVDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRINNKENEIEEEEN
                     LDNIQLEARMVGNIIKDLMKVNEDGKIQKVYDKGIDGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYDGFTDSQNENESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIEKLKKSSSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGIESLDTISQYEILKGKNFLDWIMPCVLRHRDLSN
                     LLEEVGLDAVFNVEHNSKWYGKLWNKNDILVEKKSDEEKESIEEILEKIDVDNPDSDY
                     YSEIEEKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEEREKISGTERGTIVHLVMEVLDLKNVSSVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFASNIGKRMLNAEIINREKSIYAQVNMKDIYIYEKLINNDDKKLYDNESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            complement(98586..102053)
                     /gene="addB"
                     /locus_tag="MJE33_000097"
     CDS             complement(98586..102053)
                     /gene="addB"
                     /locus_tag="MJE33_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HBY9646081.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EAENRVSKLFLGKEKDKYLRVRVLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQSGFVSSITDMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDINYIGYSKSDMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSNPTENIKIKEFNNLYSEVEEIAREIVHLVR
                     DKNVRYRDITIATRDLNRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISALEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINLLENYVLANGIKGKKWFDEKWDYRITQSLSGQ
                     ESEFELELKEKINEIKNRVLEPIVILQEKLRGKNRVKEICRYIYEFLLDINMPETIES
                     LIVNFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSKTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDKDILKKITVKSPTFNELINIIKNYDSDGYNTEEINSIWLDIYR
                     YYLKDEVYSSITKKVIKGLSYTNQVHKIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKAKKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLTWRDIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGNNG
                     KYPPIKMVLENGQEVSLIGQIDRVDEFEEGENKYIRIIDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDEDININPAAILYCRINNPIAKFNEDKDDEEIQEAILKELRMKG
                     LVVKDSHIVKEMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVRH
                     AIKDLCEEMLSGEIRIAPYKHKDGTSCDFCDYSAICQFDSTIKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            complement(102043..102873)
                     /locus_tag="MJE33_000098"
     CDS             complement(102043..102873)
                     /locus_tag="MJE33_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBY9646082.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDDKNSKKFLKWMENIKKVVPIEKGKTYL
                     PEYEVRMTLNGDIIQAKYDLVVIGNNNIEIWDWKTESKKIDYKHVENRIQTIVYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYDEEKHEANRNKIQNYISMIKNTN
                     YEEHTYEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            complement(102982..105201)
                     /locus_tag="MJE33_000099"
     CDS             complement(102982..105201)
                     /locus_tag="MJE33_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBY9646083.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKGGKILILTYMNSAVNNFKGRIRKILEENKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEITLEAWE
                     RGFFEFAGNAISELKYKGISPDKLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQTRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQ
                     KECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDD
                     VEKLIVVLNKVFIDTDNEEEIKYFLEELKNYSTEEIIYNFEFSKLDNDLIEFKEESDI
                     YLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLNLEKEDKAVLDYISFYIKYLVSENI
                     NMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLSRLNQHIIEKRSN"
     gene            complement(105194..107107)
                     /locus_tag="MJE33_000100"
     CDS             complement(105194..107107)
                     /locus_tag="MJE33_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646084.1"
                     /translation="MEYLFEKSSLKEYGNEALDKYRELLDKTGNNSRCILLLVPNNNT
                     KIRYERSLRLNYSEELKITTYISFVKKELVKYWPLIIEKCDEISTKVVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFEDITGTNKSIANSIMTNINKSAFNLIDFDFIGEKIYSSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNALSIYLYNQYLLNDDFYLKNLFTEVR
                     YIIVDSLESSSNAEVDFITEALDNTLQSYIYFDYSRDYSVFNNVDIDYINEKIISKIK
                     PKEENIKKKEIRIEDLYLLPANIELNQSSQLYNEMLDLISEKVISLIEEDVSPRDIAI
                     ISPINNSILEHQIRDSLIEKNIDVFSTKKDKKAVDYPYGNALVVATCIFYGYLDFIKD
                     DEYLSFLETLLEINRIKAFKIFNETRHLSIDNIDEHSQYREILQYIEEKKKSDIKIHE
                     FLTQFYIDKMLNLKEGKKNVGLCKKIIAESESFSESLSLLGMKKEKIFIEALKSTIND
                     YYSVVDIEELKSKDKIVITTPYSYISSNIDRSIQIWVDIGSNAWNMKIEKDISNLIVL
                     RKSFEEKKIYTNEMEEYYKKYYLYNTVYNLLLNAKKIYAFKSEYAINGYIQESILYSI
                     LLKISHKGDKNYD"
     gene            complement(107349..109361)
                     /locus_tag="MJE33_000101"
     CDS             complement(107349..109361)
                     /locus_tag="MJE33_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBY9646085.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSKSKVSVKFKEKPDADSITLKYKCYDMPLNTTLNYNQSTGAYEGTINYNQDPE
                     YLNVWELQGITINSKNNPKTLNRQDLEKLGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSSPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPKDIIIIGDENAISKTTANQIKSTVNASQTRLNGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKDSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNGITKDSVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKDNLEAKSANKVY
                     KIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSAPGNSSGGMIEKD
                     YNLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLKPDLFTSIHYNGSTNK
                     QGHGVEVFYKLKDKNGGITKTVATNILNRILEKFKLTNRGIKTRVLPSDSTKDYLYVL
                     RSNDMPAVLVECAFLDNENDMSLINSSAKVKEMGTQIGKGIEDSLK"
     gene            complement(109866..111686)
                     /locus_tag="MJE33_000102"
     CDS             complement(109866..111686)
                     /locus_tag="MJE33_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBY9646086.1"
                     /translation="MTLKYKCYDMPLDTTLNYNQSTESYEGTINYNKDPEYLNVWELQ
                     GITINSKNNPKTLNKQELEKMGLNLKDYNVTQECIIEDITSRKDVNKYLRKTSAPITE
                     LTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPILLVEKNNV
                     PNSVKSELKRLNPRDVIIIGDENAISKTTANQIKSTVNASQTRLKGSNRYETSLLIAK
                     EIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKNSITDNTYKWLKSEDL
                     QNAYFIGGPQMISTNVINKVNDITKDNVTNNRVYGADRHETNANVIKKFYTDDELEAV
                     LVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKENLEAKSANKVYKIGGGLTS
                     KVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSDTGTTGKPLGGIREKDYTLNT
                     SLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLRPDLFTSIHYNASDTTGNGVE
                     VFYKLKDKDGGTTKTVATNILNRILEKFNLKNRGAKTRTLSTDPTKDYLYVLRNNDMP
                     AVLVECAFLDNEKDMSLLNTSNKVKEMGTQIGKGIEDSLK"
BASE COUNT        35035 a        20160 c        11728 g        44811 t
ORIGIN      
        1 acatattatt cacaaaatag aatagctaaa aatatttaag aatattttct attaaatttt
       61 tattgaaata caattttttt acaaagtatg atatattata aacataaaac tattgtttta
      121 catgacttat catgacatct tttgtttcat tcacctcaaa atggggtatg ttacttatat
      181 aatgataaat tttaggaggg ttaaaaatga gtaaagtttt tgaagtaaaa aaagatatct
      241 actttactgg agtagtagat gaaggtttaa aagtattcga cataataatg gaaactgagt
      301 ttgggacaac ttacaactct tatttaatca aggacgaaaa aacggtttta tttgacactg
      361 taaaggcaaa ttttaaagat gagtttttaa gtaatttatc agaggttact gatatagcta
      421 aaattgacta tgttgtaatt catcacacag aaccagacca tgcaggtagt ttaaaatact
      481 tattagatat aaatcctaat atagaagttt attgtactaa agctgctaaa ctatacttag
      541 atggtcaaat aaatagacct tttaactgtc atgtaataaa agatggtgaa attttaaata
      601 tcggtaaaag aaatcttaga tttataactg ctccattctt acattgggtt gatacaatgt
      661 ttacttacat tgaggaggac aagactcttt taacttgtga tgcatttggt tgtcactttg
      721 caagtgttga tgcagaggta gtgaatagtg aagattatct aaaatcagct aaacactact
      781 atgattgtat agttaagcca tttgctaaac atgttttaag tgctgtagat aaggtggtag
      841 gactaaacat agaatttgat actatattga cttctcatgg tccaatgctt actaaagacc
      901 caatggctgc tgttaaaaga tatgttgagt ggtctacaga agctgttaat acaactaatc
      961 aaaatcaagt ttctatattc tacttatcag cttatagtaa tactttagag atggctaaaa
     1021 agataaaaga aggacttgat aaagaaggtg caaaggctga attatatgat ttagaagata
     1081 tgacattaac agaaatgcac gatacattag tagtgtcaaa agtaatatta ctaggttctc
     1141 caactataaa caaaactatg gttaaaccta tgtgggattt attctctgtt atagacccaa
     1201 tggctaacca aggcaagata gcaggagtat ttggttcatt tggttggagt ggtgaaggta
     1261 taactatggc tgagacttta cttaaatcta tgtcattcaa aatgcctgta gaatctctta
     1321 agaaaaaatt cttccctagt gaagagacat taaaagagtg tatggcattt ggtgcagaat
     1381 ttgcaaaatt agttaaatag ccattaatca aacataatat tatttaaaat aaatgatttt
     1441 atatttaaaa ataaattaaa atggagttgt ctcaaaatta atttttctaa tctaatttta
     1501 agttcaactc cttttttatt ttctaaaatg actgatataa tgataaatgc accctttgtg
     1561 cgagggtgca ttatttataa tatgttctat ttgtacacta gtatatttgt attaattcac
     1621 atatctagcg tatgctacca tccctcttcc agaattagat gccgtttcac caatttccac
     1681 tttaataatt acattatttt ctttacactt ttttgataat tctgctgcag aaaggttctt
     1741 accagtaaaa tctaaagaaa atggcttatc atattcaaaa ggatactcat caggtacacc
     1801 atctggagct aatgcaacca taccatatgg tgtattcata aaccctatta cagtaactgt
     1861 atgctttgat gggtctaaat ttgaattttt gcttgtaaac ttaattgtat ttattgatga
     1921 gttgattaaa ctacacggaa ctccctcata catttcaatt tttggacgta gattatttcc
     1981 ataaacttca tcaagtattg ccccttgttg atctgctatc atagtgtgat tcccaggtcc
     2041 aactggcgca gaaggcttag ctggcgtagg tttcttaaca gttggcgttg gattctgtgt
     2101 ctttactact tcgccaacag ctggatatgc tttcttgtca aatggtaatg ggtgagtaag
     2161 actcttgtat atgatgtctg ctcctgctga tttgttggca tcaatatcta catgatacaa
     2221 acgtccatcc tcaaaacgaa taagattcca ctgatggtca tttgttgtac tatgtacata
     2281 ataacattca attcccatag catcacataa agctttcaag ctcctacaca ttccagaaca
     2341 agcacctttt ttagtaacca atgtataagc agtattggct ccctctgctg cgttccagtt
     2401 aacatatgtt gtgttgtttt taatgtgatt ataagcaacc ctaatcttag catctgtggt
     2461 catgttcttt gtaattttct tgttcataaa gtctgctgtt gcttgtgcta ccgaattaag
     2521 ttgagcttgt gataactttg gaccatatat actgttggtt ccagctacat aacttttcgg
     2581 tgtctttgca gtaacatctt tagctgtcat accataaact gttacactca tacatgaggt
     2641 taaaaccatc attgcaatca atagtttagt agctaatttc ctgatttttt tcatattagc
     2701 attctccttt ataatatatt ttactttcaa ttccctacca ttaattaagt tcattttaga
     2761 tttaaaatat ggattatcaa catgtatgca tagcaattca cgtggtcata gaatatttag
     2821 atttcttgga aaatctgttc tatatttcct atatagttcg tatacaatcc aactcacaaa
     2881 cctgcatttt tgatatcatc acaataaaat gataataatt aatatattca aaaatgttgg
     2941 tctgtaacag gtatgtcaat tttccaatta ttggtaatgg cgagagtgag gagaattgtc
     3001 aataatacat ttaaaattaa aattgtatac acttcataca ttctccccta acaacattat
     3061 acagattata atttgtatat tcaatacatt ttttggaaag tataattata tcaaaatatg
     3121 tgattatgta tagatacaat ccactatgga gaaatataaa agaaaagagt ataacaaaca
     3181 ataaaaaata agatgattca aattttattt tgaaacatct tatttactct acaaattaag
     3241 attatttggt gaattattta attattattt tttattaaag aaattttaaa tcttaatata
     3301 aaatacgcta ttatacataa taatccaaat gcaaatgcta ctggaattaa catctccgat
     3361 tcaaaataaa agaaatattc taagaaaata tctggattta ctatattccc attcttgtct
     3421 aaaaaatctt ttccattata tcctaaatgc tgtataaaat tacttatgtt attataatct
     3481 gcttccctct catataccat attagcattc aatccagaag aacttaaatt agtgtttcca
     3541 tctttcatat ctattacata tagtctaaga gctttgactt tactatactt atctttatca
     3601 agtatttttt caagttctaa cttatattca ccaatctttg tcatatcatg tctttctgcc
     3661 aattgcttta tttctccatc aagttctttt cctaaattat acatattatg ttgtaaacta
     3721 ttattgtaat accacataaa tccagaaatt aaaaatgcaa ttatacttat gtaacctaat
     3781 acttttttaa accccattac aattgattta tgcctatcca atgataaaga tagttctttt
     3841 attatattac ataattcata ctgcatttca tcaccatcat caaatctttc aattgctttt
     3901 ttacatgctt cttcttcatc taatccttct aatttaaatt catttgcaga ttctataaga
     3961 tggcatctca tttcctgttt taattctata gtacttttat tgtcacattt tttatatagt
     4021 ttatctaaat attcatctat tagtttcata aattaatcca cctctcccaa aaattgattc
     4081 ataacctttt taataaatct ccattcttgt ttctttattt ctaaaaaatc tttaccttca
     4141 ttagttaaat tataatattt tcttcttcca cctgaacttt gttcattgct ataataagat
     4201 ttaattaaat ttttagattc cattctttta agtgcaagat acattgttcc ttcttttaat
     4261 tcaaactcac ttctttctct tacttgcttt gcaatttcat atccataaca atctttcttt
     4321 tctaatatag aaacaattaa tatatcgata taacctttta acacttcttt atttaactgc
     4381 attatgtacc accgcctttt cacctactac tctataatgt atagtactta tatttactat
     4441 aataatacat agtactctat attgcaaggt ttatattcta caatagactt aaaaataata
     4501 aatagctgta tattaatatt taatctataa atactaatat acagctattt atcaaatttt
     4561 aaaaataaat ttttgtttta ttaatgttgt cataacaaca tttacttggt tgaaatagta
     4621 ttacctataa gtttaaatat atataataat agacttgatt ctatttcata ttattaaaat
     4681 agagtaatag tttaaattct actactctat ctataatcta tttcctaatt atttaatctc
     4741 aaatttagct cttcatcatt taaatctttc ataagatttc tacaggtact agatttattc
     4801 caacagaaag actagggcat taaacactta tatatctgtt tctttagact gtaatattaa
     4861 tattttttaa atacgttgac ttttgctttt tctaacttct ctccatgata gttctcctct
     4921 atcaagagct tttataaata tttctgctga agctagattt gtagctattg gaacatgata
     4981 aacatcacaa agtcttatta acgcttgtat atctgtttca tgagcctgag atgtcaatgg
     5041 gtctcttaaa aaaataacta aatctatttc ttgtgttact attaaagaac ctatttgttg
     5101 gtctccacct aaaggaccag atgctaatct atttatattt aattcagttt catccattat
     5161 tctttttcct gtagttccag ttgcatatag tccatacttt tttaaaattg attcataacc
     5221 tatacaaaat ccgaccatag tgtttttcat ttggtcatgt gctactaatg ctatattcat
     5281 atatctaatc tcctcaattt atgtttagat gtatttaaat ttagaaattt attttctttc
     5341 ttctcataca cacatttagt actaatccta agtttgctaa agatgtcaat aacgaacttc
     5401 ctccataact tacaaaaggt aatgttactc cagttactgg tattaacgca accgtcatac
     5461 ctatattttg gattatctgg taaccaaaca ttcccataac accaaccact atgagggttc
     5521 cataaaaatc ttttgcatct cttgcaattg ctaaaagcct taataggaat atcataaata
     5581 agattatcaa cactgccatt cctattacac caagttcttc acctacaaca gcaaatataa
     5641 aatcactatc ttgtacaggt aaaaaatctt cttgattttg acttccattg taaagtcctt
     5701 taccagttac gcctccagaa ccaattgcaa tcagagattg cataacttga tagtttcctt
     5761 ttagagttac atcttctgga tttagaaaag cttctattct taccttttga tggtctgcca
     5821 tcattaaata catcagtggc attgacacta atagtatgat tattcctctt tttatcaact
     5881 ttgagctaag acctgctgtg aacagcattg caaatatcat acaacaaaat acaattcctg
     5941 ttcccaagtc tggttgtgca atcaataatc cgataaatgg aagagaataa actacaacag
     6001 gcataacttc ctttaacgta tttaatttat cctttttgga ttcaagtatc tttgcataac
     6061 ttagtacaaa tgttaatttg actatttcag atgtctgcaa gtcaagtggt cctaagttaa
     6121 tccacgacct agctccacct tttactgtcc caattccagg taataaaact atggctaaca
     6181 aaataagact tattatataa agcgctttat aatacctgcc tagaaggtta tagtcaaaaa
     6241 ataatatgac tataatcatt cctatcccaa gtacaaaagc taatccttgt ttaatcaatt
     6301 gattatatga acctgttgaa ttagcatgtg ttgcactact aagtataaca agcccaaaaa
     6361 taaagatagc taatactgta actataagtt tccagtctaa ctgttttatc aacttaattg
     6421 tcgacttata attaatattg atttttttca aagaatcacc gtctttctat ctttaaacat
     6481 ataattaaat aaaaggggca tataccaata aggatatccc ccttttaagc actaattaca
     6541 tacatctatc ttttatattc tttagtggta tattagcaac taatgcagat acaggtttgt
     6601 catcatgctc acctctgcat ttagacattt ttatttcaag accatcatct tctatttctg
     6661 cataattagc tattactttt aaaatgtctg tttttatcat ctctaaaagt tgtggtgaac
     6721 aatctactct atcatgaact aaaactagct ttaacctctc tttagcaaca gatttactag
     6781 ttttagcctc gttagaaaaa actctaaata aatctaacac agatgtatcc tcctttgcta
     6841 cttagccatg ctaaacattt ttttaagtct accaaagaat ccatcttcaa cttcaagatc
     6901 aagtagagga acttcttcat ttagtatcct ttttgcgata tttttgtatg cttgaccagc
     6961 aagtgactta gaatcaagta tagctggctc tcctttattt gttgatataa ttatgctttc
     7021 atcatcagga acaagaccta acaaatctat tgctaatatt tctattatat cttgtttatc
     7081 catcatgtct ccacgcttaa ccatatcatt tctaattcta tttataacta atcttatctc
     7141 ttttatttca tttgcttcta atagacctat tattctatca gcatctctta ctgctgatat
     7201 ttctggatta gtaactacaa tagctctatc tgccccagct actgcatttt taaagccttg
     7261 ctcaatacct gctggacaat ctattattat atactcgaaa gattctttta gtttctcaca
     7321 caggtcaatc atctgctcaa ctgagacagc atttttatct ctagtttgtg ctgctggtaa
     7381 taaatataga ttatcaaatc ttttgtcttt tattagagct tgcttaagcc tacaagttcc
     7441 ttcaactaca tctactatgt cataaactat tcgattttca agacccatta ctacatctaa
     7501 gtttctaagt ccaatatctg catccacaac tactgttttt ttattttcta ggcttagcgc
     7561 agttcctaag tttgctgcgg tagtagtttt tccaacccca cctttaccag atgttataac
     7621 tataacttcg ctcataatac taccccctat ttttctttct atttgtctaa ctttgacaaa
     7681 tagctttcga ttacaattct tccattactt acaaaggcta tttctgggct aatttctgat
     7741 tcactttcat aattttcttc atcatctgga gcttctgcaa tattatctgc tatttgtaat
     7801 attttaggat taagatttcc agcaacaaca tatgcaaatt cattgccttc tgcacctgca
     7861 tgtacaaaac cttctatttt acccattaca aaaacattac ctcctgctac aaccttagca
     7921 ccagcattta tatctcccat aacaactgta tttgattttg aaaggacttt tgaaccaggt
     7981 ttcatatcat tcaatataac tacatcacct tcaaattcaa tattttcacc agatcttagg
     8041 gtattgacat attttgttgg aaaattacta ttttcttcaa ttttttgatc ttcaacaaat
     8101 tccacatcaa atctagaagt aattccttct ttaagttcta gaatctccat gtcagttaaa
     8161 caatcactat taattttact aattttagcc ccattaaaaa agccaacata tgcttccaat
     8221 ttgtttatta ttttttcctg tatttcctca aatggagctt ctctttttat atttactatt
     8281 atccctcttt tattaccctt gaactctact agttcttgac tacaaatttc tcttaaagac
     8341 ataacaacct ccagagtgat atatcattta tattcttcaa atacgcttta aaccctttta
     8401 ttttttctat ataaagctaa agtttataca tagattgagc tatctattaa tttgtgactc
     8461 gaaatttata ttttcgtcag aagaattgtt aacatcattc gtcttgttgt catttttact
     8521 attagttggc tctaatccca tataagttcc aataacttct ctagttggta aaagtgagaa
     8581 tacacttgaa tcaccttgag gaattacaca tacaacagct atttctgggt catctgctgg
     8641 agcaaaagca acagtccacg taaaagaacc ataatcttgt ttaaatctat ctatttggtc
     8701 atctgttatt tttggattta attcttttat agcttttctt agatatgaag aattcacctt
     8761 atcagtgtcc tctaatctaa cttttactcc atcttctaat tcttcctcta atttctttct
     8821 ttcttcgtct ttcaaatctt tattttctaa tttctttttt atttcctttt ccttagctaa
     8881 tgaaagctct ttttcttttt ctgctttcat cttatcagct agtttaatcg catcatttag
     8941 gttgacatta taagatgaca tatgactttt taaatattca tactcgttat ctgtaggtat
     9001 tttcccactt ttctcagctg taccagtttt tgctgctaca tctattggaa aatcagcaaa
     9061 agcactcttc gctgtacctt gtcttgcaac caatttcata ccttttgtta attcttttaa
     9121 gttatctgga tttttaaaag gtattttttc gactttcttc tggtcattaa ttttaactga
     9181 tgaataatca cttgaaacag ctctatcaac tacagataat tctactaaat tacctccatt
     9241 tgctatagca gctacatatc ttgatatttg agctggtgta taagcatttt caccttgtcc
     9301 tatagctaag ttgaatgtat ctgcagtact ccattttgca aaattaaaat aactaaatac
     9361 tgctaaatct gctacttctt caattctatc ttctttaaca tccaattttt ttagcctatt
     9421 cattgtttca actctaccag gagttttctt ttctgctgcc cagcttacaa tctcattgat
     9481 tcttttctca tattctttag gatttttttc tctagttata tcaacaaaat catttgccat
     9541 ttctctttct aaatttgact ttagaagagc ttgtgttgat ttcaatttat cttctgtgct
     9601 aggaacttta cctgctcttt cttctacttc atcttgaagc cctgtatttt ggtcaagccc
     9661 aaataatttt gcatactcaa gtattttatt tgcattcata tctataccaa tgctttttcc
     9721 atctggccat gttttacctg taccaattgt tgccatatat atattacaag actcttgtat
     9781 tgctttatat aagtttgtca taccatggtt tcctctacca tggttccata catagtcacc
     9841 aaaagatttt ttacctaaca ttataacccc agggtcattt atagtaaagt ttggatccag
     9901 accattttct aacgctgcca ttgaagttaa catcttaaat gaagaacctg gctgaaattc
     9961 cccttgtgta accaagttta aaagtggact tcctgctagt aagtcatttg gattttttgg
    10021 ttgaagtgct ttataatcct ctgtagaaat accttttgca aatttatttg ggtcataatt
    10081 aggataactt gatgaagcta atacttctcc atttttaaca tcaatagcta taagagctgc
    10141 tgactgtgct tttcctgcat atgcactaat tggtttgtcc ccaaatttac ttttaaaagt
    10201 acctccttta gaagcaactt cgattatctg ttttaatgca ttatctgaaa cttcttgtaa
    10261 atctttatct agtgtaagat acacagtatc tcctgattta ggttttttag attcaatttc
    10321 tttactgatt ctacccaatg catcaacttt aaccatttta taaccatcag ttcctcttaa
    10381 tctactttca ttactctttt ctactcctgc aagacctacc atatctccag tttcataacc
    10441 tttttgaagg tatgattcta tttgagtgga tggcattttt cctacatatc ctaacatatg
    10501 agaagctaat gacccattag gataatatct tactggttct actgctattg aaacaccaac
    10561 aaggtccata gcactttctt caatttgagc aatcgtagtt tctcccacat ccttagcaat
    10621 agttactgga ttatattgag aatatccttt tgattttatc aaatccctta cgaccattat
    10681 ttttcttgca tcttcatcac tcaaactttt atcaatttct aatgcatcac tatttcttac
    10741 tttttcaaac gcttctttgg cactcagttt agtttctttt atcttataac tagccaacca
    10801 atctctctta tctctttctg ctgtgaacat ccatttacta actaaaatag gtggataata
    10861 tccattttca tttagttttg cctgtaatct tgtcgcatcc aatcttttat cttcttgaga
    10921 taatattcca gctgatataa gtttgtccac caagtaataa aaactttcct tagcattata
    10981 atcatttgga ataccatttt ctgatttata ttccctaata tctctgtcat atgtataata
    11041 gtactttcca ttttctacat atataggaaa ctcatctaca tattcttcac catttctctc
    11101 cagcaaattt ataagtttta atgatatttc atttgctctt gaatgcttat ttgcatcttt
    11161 cttattaata tcgttacctg aaacttgaac agtaaataag tttttatttc ctgccaacag
    11221 tcttccatat ctatctttta tttctcctct tggagcttct attgccaact ttttatatgt
    11281 tttattctca gctaactcat tgtaatattc atatttaaat gtagtcatat agattatttt
    11341 tactaaaata gctaaaaata tcacgcccat gactatctta attattttta gtctatcaac
    11401 ttgtttattt tcttccatga aatcaccaac taatcttctt taagttttaa aactgctctt
    11461 ttagataagc gatatattac taaaccaatt aaactattca ctattggtgc tactagtaga
    11521 ccttttacaa aaagtgttga tatgttgtag ctttgataaa ttatactaga tgtacctata
    11581 ttgacaattg aatcaattaa actgcctata aaaattaaaa cgaaagtaat aatataacta
    11641 cctttatata gctgatctct catgtatcca attgcatatg caataataaa caaaattaat
    11701 gcatttacac caaaaattcc accaacggtt atgtctttaa taagccctat tattgctgct
    11761 ataatcccac cttctaattc atctaaataa agagagatta tggttacata tataagaact
    11821 aaattaaagc ttatgccaaa aatatctata taatttgtta tactattttc tatagtcata
    11881 agcacaatcc ccaacagaca aagtaaaact tttttcattg tttttatccc ctttatccta
    11941 tatttcttgg ttctatgact acaacgtcat ttatattctt aaaatcaaca taaggtttta
    12001 caactacata ttttaatgac ttatctttat catcaaccac tttttcaact tcgccaattg
    12061 gtataccatc tgggaagaat ccaagacctg atgttgttat tatatctcct tgaattacat
    12121 tatatgatga gtcaaacata tatccttgca aatatccttt actattataa ctatctttat
    12181 tatctaaagt agtattttga gtaattgtac ctttagcatt ggcatccttt gctagtttaa
    12241 agcttacaga tgccttagaa tccaatagtg aaattgcctt actataattg ttggaaacct
    12301 cataaactat gccaactaat ccactaccat tcataactat actttctttt tttacacctg
    12361 aattttttcc tgcacctatg acaaaactct cataccagtt tccatcattt ttaccaacaa
    12421 cacttgctga aatacttgtc gccttatact cttcttgtac aaagttcagt gtctttttta
    12481 actcttctag cgattctgtt ttatctagtt tggcatttaa agctatcata ttttttttaa
    12541 gtttttcatt ttcttcttct aattttttta cctttttaga attatcttta aaatttgcta
    12601 catctttaaa accatttttg ataaaagaaa atccatcatt tattccctta cctacacttg
    12661 taatagcatc tgaagctact cctaaattta ctggactacc actagaaaat ttaccaattg
    12721 atatacccac aattccaatt aaagtgatag caactacgcc cgttgctatc actttaacat
    12781 taatcctttt tttctcattt ttgttcttgt caaatctcaa ggccatcacc aacttctaat
    12841 tcttagtatt catcattaat actttttcaa agatttcttg gtcttcaaca gatttacctg
    12901 ttccaagtgc aacacaatca agaggtgttt ctgctatttg tacagacata ccagtttcct
    12961 gtgtaataag cttatcaaga cctctaagca aagcgcctcc tcctgttagc attattccgt
    13021 tttccattat atctgaggct aattctggtg gagttctttc tagtgtagat tttattgcat
    13081 ctactattga acttacaggt tcttttaatg cttctctaac ttctgtagag catatttcta
    13141 ttgttttagg taatcctgag attaaatctc tacctcttat ttgcatattt atttcttcat
    13201 catctttaaa tgtagaacct atgttaattt ttacattttc agcagttctt tcacctatca
    13261 taagattata ttcttttttt acataagcca ctatagactc gtcaaattcg tctccaccta
    13321 ttcttataga tttagcagta acgattcctc ctaaagatat tacagctatt tctgaagtac
    13381 ctccaccaat atcaactacc atattacctt ctggttcttc aacttttaat ccagccccta
    13441 tagccgctgc cattggctct tctataagga atgcatcttt agctcctgct tgtcttgcag
    13501 cctcttctat agctctcttt tctacttctg taactccaaa aggaacacat atagctattc
    13561 ttggacttac aacacctttt ttatcagccg ctttttgtat aaaatagcta atcattgatt
    13621 gagttatgtc aaaatcagct attactccat ctttcatagg tcttatagct actatatttc
    13681 ctggtgttct ccctatcatt tttttggctt cttcacctac tgctaatacc tctttactat
    13741 catctcttat tgcgacaact gatggttctc taacaacaat accttgtcct tttatgtaaa
    13801 ctaatgtatt tgcagtccct aaatctattc ccatatcctt tgtcatctta ttaaaactaa
    13861 aaaaaccttt tttttctttc tttttttcct tagccatgat tttactcctt cctactatta
    13921 agaatcatgt ttaaaacatt ctcctattat tttatcacat ataaagtcaa attatcatat
    13981 tttctttaaa gctaaagtat aatccatctc caataattat atggtctatt atttctattc
    14041 caattatttc tccacattta ataagtcttg atgtgatatt tttatcttct cttgatggtt
    14101 ctacacttcc tgaagggtga ttatgcatca ctattatctt acttgcactt cttcttatag
    14161 cttctttaaa aacctctctt ggatggacta atgacgagct taaagtccct atagatacat
    14221 ctacatcaga tattatctca ttttttgtgt ttaaaagaac cgctttaaaa acctcttttt
    14281 ttaaatacct taggctatcc atataatacc tctgtatatc ccaaggattc cttattttat
    14341 actttatagg tttaaaacta gctactctag agcctagttc taaagctgct ttaatttggg
    14401 ttgattttga taacccaatt ccatctatat tacacagctc ctctattgtc atatcttcta
    14461 aatgtctaat accctgaata tctctattta ttatatagtt ggctaattca atagcatttt
    14521 tattcttgtt gccagttctt aaaagtatag ccaacaactc agcatttgat aagcttttta
    14581 cgcctttggc aagcatcttt tctctgggtc tttcctctgg tgccatttct tttaccttaa
    14641 tggttgaatt aaatgacttt ttcagataaa tcacactcca taaaaaagat ttatactaaa
    14701 atattttttt aataggtcgc ctaaccttga aattggaaga ccaactatat taaaataatc
    14761 tcctctaatt tcttcaacta acaaagctcc ataaccctgt ataccatagg ctcctgcttt
    14821 gtcaaatgac tcattagttt tcagataatc ctcaatatcc tgctctgata acgttttaaa
    14881 tttcacattg ctgattacat agtcaattac ctttttatct tcgcacaagt ttattaagct
    14941 aatacctgtt atgacttgat gctctcttcc agatagtctt tttaacatat ctcttgcaca
    15001 agactcatct tttggttttc ctaaaatagc attgtctaaa acaacaactg tatcagctcc
    15061 aataacaaca tcttcattat gttcagatgc tacagacata cttttttcaa aagctaatct
    15121 cataactaaa tgttttggtg tttcaccttc caatataatc tcatcaattt catttttaat
    15181 tatatcaaat cttacattcg tattctctag aatttctttc cttcttggag aggctgatgc
    15241 caagattata ttcatttatt cacctcattt gtacgtaatt tgtacctatt tagtctatca
    15301 ctatattgta aaaaattcaa cagaaatgtc gaaccttttt tgttacacta ttgttatatt
    15361 tattaccatt agaataaata tacataaaat agaggtatgg aaattattcc atacctctgt
    15421 tttatgtact cattagagat atggaaatta ttccatatcc ttattttgtc catattacaa
    15481 aattttaata gctttctttg gacatttttc catacataaa tggcatccta cacatttatc
    15541 tgcatctacc ttatgttttt cttttaattc tccctctatt gcatcaaact tacattgttt
    15601 tttgcacact gtacatccaa cacaaagttc ttggtcaata gtaactttct ttttcttagt
    15661 tatgtcacct gatattactt ttgtagggca ttttgctaca cattgcatac aacctacaca
    15721 cttattaggg tctatcttag ctattttgtc ttcaaatatt attgcatcaa atttacaagc
    15781 ttttacacac attccacagc caatacatcc agcagtacat ttttctttaa cagctttacc
    15841 aaactctttg ctattacact ctacaactac ttcttgactt tctggttttg tgattattat
    15901 acccttagga caaacttctt tacattttcc acaatttaca cacttttctt catctattac
    15961 tgctattcca tcaactatag aaatagcatc aaatttacaa gcatctttac atgttccaag
    16021 acctaaacat ccaaatttac acatcttagc acctgagttt aaaacatttg ctgctctaca
    16081 atctgatatt ccttcatact catatttatc ttttgcactt gaacaagttc ctttacaaat
    16141 aacttttgct acttgtttct cacctgcttc agcacttatt cccattattt ctccaacttt
    16201 tgcaccaaca tctgctccac ctacagggca accattaact ggtgcattgc cttcaactat
    16261 cgcatttgca agacctccac aacctggaaa tccacaacct ccacagttgg ctcctggaag
    16321 tactcctagt atagcttcca ctctctcgtc tacttctaca gcaaactttt tagatgcaaa
    16381 atctagtact atcccgaaga ttaagcccat aattcctaaa actaatacag ctgtaagtat
    16441 caccattttc acccctcttt tttatagctt tataagtcct gtaaatccta agaaagctat
    16501 tgacattaaa cctgctgata taagcgttat agggaaacct ttaaatgctt ctggtatatc
    16561 agctaattct agtctttctc ttatacctgc gaatattact attgctaaag taaatccagc
    16621 tccggctcca aatccgttta ttattgtttc tactaaatta taactttttt gaacattaac
    16681 tagagctata ccaagaactg cacagtttgt agttataaga ggtaaataaa ctcctaatgc
    16741 ttgatataaa gatggactca tcttttgaat taccatttca acgaactgaa ctatagaagc
    16801 tataaccaat ataaaagcta ttgtttgtaa gaatccagtt ccagttttta ctaataaaat
    16861 ctgtataaaa taagttataa ttgaagctag agttaaaacg aacgtaactg caactcccat
    16921 ccctactgct gtatcaacct tcttagaaac tcccaagaaa ggacatatac ccaagaattg
    16981 agatgttata acgttattaa caagaacgat acttaaaaat aaaagtatta aattcattta
    17041 ttacacctcc actatgcttt tttacttctt aatttattaa atcctgcaaa taagaatcct
    17101 aatgttaaga atgcacctgg tggtaatata aatattaata ctggttggaa agatgctcca
    17161 aataatgtca ttccaaataa actaccattt ccaagtaatt ctcttactgc acctattatt
    17221 gtaagtgcaa cagtaaatcc aagaccttga cctacaccat caactattga aggcatagct
    17281 cctgttttga aagcaaaact ttctgcacga gctaatatta aacagttaac aactattaat
    17341 ggtatatata taccaagagc cttatcaaga gcaggaacat atgcttttaa aagcatacct
    17401 actattgtaa caaatgtagc tataactact ataaatatag gtattcttat cttatctggt
    17461 gtaatttttc ttattaaaga tataactaaa tttgcacata ttaaaactgc tgctgttgaa
    17521 agacccatac caattccatt tattgctgaa gttgtaacgg ctagtgttgg acacatacct
    17581 ataacttgaa caaatgttgg gttctcatct attagcccat ttttaaatac tttagctaaa
    17641 ttcataatta accctcctta tttattaaga gcactattga aaacatctat agcagcattt
    17701 actcctgtag taactgctgt agatgtaata gttgcacctg atattgctaa tatttgatta
    17761 tctgctgttg tacttccttt tgctacttca agaggctcag cagtttttcc tttgtattga
    17821 cccttaaatg catcatcctt agcttttgct ccaagacctg ctgtttctga catattacca
    17881 atgtcaacac ctgtaacttg accttcagat gatataccta ccataacttc aatttctcca
    17941 ccatatccac ttggtttagc ttttaaagta tatccaacca cttcagaacc atctaaacct
    18001 tcatacactt cagctataag accatctcca aggcctttgt aagtatcttc cttcatttct
    18061 ttaaattctt ttgcttctgg aaggactgct tttctaagtt cattatttgc ttgtatattt
    18121 ctttgttcta ttactggagc tgtaacctga ttagtagctc caagagctaa tgcagctatt
    18181 gcacttatag caagtaaagt accacctaat ctcaccatac tgttcattat ttagccacct
    18241 ccccaaatac tctatttcta acaaacttat ctataagtgg agcaagacag ttcactaata
    18301 gtattgagta agatacacct tctggatatc caccatacat tcttataaca gttgttataa
    18361 gaccagctaa tacagcgtat attatttgac cttttttagt cataggcgaa gttgtataat
    18421 ccgtaagcat gaagaatcca cctaacataa gtcctccagc aaacagttga tatattgcga
    18481 aagttatatt aaatccacct aaaaggaatg ttaatataaa tacagttgct atgtaaaata
    18541 ctggtataac ataatttatt atgcctttat acattaggta tactccacct aataataata
    18601 atattgctga tgtctcacct atacatccac caatattccc tatcatcatg tcagcaagac
    18661 taattccatt gctagaaaga tctgccagtc ctgctgctcc atttttcaaa aagcttaatg
    18721 gagttgctgt agtaactgca tctaaatttt tacctatcca gttaacacct ggttgagtcc
    18781 aagcagtcat tgctactggg aatgatgcta ataaaaatgc tcttgcagca agtgctggat
    18841 tgacaaagtt acatccaatt ccaccaaata ccattttaac tactaatatt gcaaatattc
    18901 ctcctactag acacatccac caaggaagtg atgctggtac gttaaatgct aataataatc
    18961 ctgttacaac agctgaaaaa tctcctatag tacttttttg cttcgtaaat ttttgatata
    19021 aaaattcagt acctactgta ccaagtatac aaaaaaacat agcactcaaa gcattaagtc
    19081 taaagaagta tactcctgct actgctgctg gaaggagtgc tataataact tgtttcatta
    19141 tatatgaagt atcttcatta cttctcacat gaggagaaga tgatactatc aacttatttt
    19201 ccatcgtttt tcccccttaa ctttctttat ttctgagcag cttgctttct tctctttgct
    19261 cctatttctc tctttgctga tcttatagat tgaagtaatg gtcttcttgc tggacaaata
    19321 aatgagcaag acccacattc tatacaatcc attgctctat gatattcagc tgtgtcatag
    19381 tcatctttta aagaatatgc acttatgaaa agtggttgta agaatgatgg acatacatct
    19441 gtacatcttc cacatcttaa acagttttga atatctggag tacgtgattc ttcctcatcc
    19501 aaacataaaa ttcctgaaga acctttgtta gtcgctattt cagtagtata ttgagctatt
    19561 cccatcatag gtccacccat tataacctta ccaatcttct tatcttcttt aaatccacca
    19621 cattgttcta ttatttctga aactaatgtt ccaacttttg ttattaggtt ctttggctct
    19681 ttgatgcaac tacctgttat tgtagtgatt ctttcaacca aaggcatacc tgttttaata
    19741 gacttagcta tctgtgctgc tgtggcaacg ttgtcaacaa ctgcacctgc tgctattggt
    19801 aatccaccag atggaacttc tttgcctgta cacgcataaa taagttgttt ctctgctcct
    19861 tgtggatatt tcacttgaag gccaacaact tcgatttcgc tatagtcttt agctacattt
    19921 tgaattgctt ctatagcatc tggtttattc gtttcaattc ctataaatcc ttttttgaca
    19981 tctaatacct tcattaatgc tcttaaacca aacacaacat cttctggatt ttcaaccatt
    20041 aatctgtggt ctgcagttaa gtatggttca cattctgcac catttaatat aactacttct
    20101 gccttgctat ctggtggagg agatactttt acatggttag ggaatgtagc tccacccata
    20161 cctactattc ctgcctcttt tatgatacct actatatctt ctttgctcaa atcctctaac
    20221 tgaccttttg gttgaacact ctcatgtaat tcttccttaa agtcattttc gataactaca
    20281 cattgagctg aacctcctgg tacttcatgt tgttcaatag caataacttt acctgatact
    20341 gaagaatgaa cgttacatga aacaaaacct tgttgttcac ctattttttg cccaaacttt
    20401 acttcatcac caacttcaac aataggttta gctggtgccc ctatatgttg ctgaagagga
    20461 atgtaaacta tctttggagc ttgagctttt tcaagtgcct ttgtgttaga gtactctttt
    20521 ctatatggag gatgtatccc tcccttaaaa gttaagagtt tcattgtttt acccccttat
    20581 ctataaaaaa acattttttc tgccttctat aagtatacta tatatattaa aaaatcttaa
    20641 caattgttaa aaaaatatcc gtatttttat aatttatttt ataaatcaac acctctatat
    20701 taagtcaaat tgaggaaaag gattgcttaa ttaaacaatc cttttcatat agctttctaa
    20761 agtccaaatt ttctgtattt tattttatag aatttataaa catagagtac tcctgcatgg
    20821 atgataaaag cagactttta cttacactat atttttcttc atttgctgct tttgaagctg
    20881 tatcaatttt ttcattcaca ttctttacat actctattaa attatcttta aataaacgca
    20941 tttcagaata atcaatcttt tcagcttctt ttgatatatt ttgtgatatt gcctttctat
    21001 ttgtcaaaat agtattatat ttttccatat ctacattatt ttcagcattc gaccaaaaac
    21061 ttgattcttc tttaaagttt gttattaatt tatttaattc tttagatata ctctctatat
    21121 aagcgtagtt acttgtatac tctaaagaca acataggtgc atctaagtgt gataaaaatg
    21181 catctggaaa cacctttctt acactgctta tctgctttct aacatcattt tcatcaaaag
    21241 aggaataggt ctgtattttt tttactccat ccttctccat aactgaaaaa ggtatgtcat
    21301 tattaactaa tgatgtctgt atttttttca aatcatcatt attatctatt gcagcaactt
    21361 gaatagtata catatcccag ccttctatag tagctaattt gacttcttct ggctctttag
    21421 tgttatttac cgctttttct ggattagttt cttgtttatc atctgaattg gtttgtgatt
    21481 tagatttttt cccttttttt attgaattta attcatcaga tatatcttta taagttatat
    21541 ctgatgaatt atttagaaat ggtattttag ccgatacata ctccaatatc ttgctatctt
    21601 ttatttttgt ataagaataa tagccaatta gacacaagca agcaatcgtt ataacatatt
    21661 tacgtaaatc aatcttatgt ctattattat taaatcctct gtatatcgtt tttcttctat
    21721 tcattagttt gtccctccca aaattatatt attaataaag tatattttta agagtcacaa
    21781 tttatgaata taaataatta tttcttctaa ttttttaata agaaattgta cttactaaac
    21841 aaatcttctt gcaccaagta atactttaga ccaatatgaa ctagttaaat tatccacttt
    21901 aaccttatta ggattagtct gagtaccact acaatggata aatttactat ttcctacata
    21961 tatacctaca tgattagttg ttccatcacc atcagtgtca aaataaacta aatctcctgg
    22021 tgcataattc cccatagaaa ttgcacttcc aaaattagcc tgttctctag aaactctagg
    22081 aatagaaacg cctacagaat gtttaaaaac atactgtgta aatccagaac agtcaaattt
    22141 atcaggccct gaagctcccc attgatatgg tattccaata agagtatatg caaaatctac
    22201 gattgttgaa ccttttgctg tagatttatc tgaatttcca gtaaatgatg gctttgagtt
    22261 actattaggt tgagaatttt ggttagatga tgaatttggt ttttcttttg taccttcatt
    22321 agtctcagaa atataacttg cactcaccca tccaattgtt ccactcgata actttacctt
    22381 ataccaacca ttacttttag cttttaattc aacaacatct ccaccattta attttcctat
    22441 tatagaatag cttgtcccag gaccacttct tacatttaaa ctcacagcag atgttacctt
    22501 accattcttg ccttcaattg attcttcgct agatggtttt ttatcagaat tattagtact
    22561 atttgaatta ctagaattgt tcgttccaac atcttctgat gttttagaaa tatattggct
    22621 actagcccat ccagtaactc catttgatag ttttatttta taccatccat tgctttgttc
    22681 tagtaacttt actatatctc cattatttgc tttaccaact aatgaatagt tagtacctgc
    22741 accacttctt acattcagtc tagaactcac atttacttta ccatttccac taatagttgt
    22801 cccacttgaa gttggattat tttgacttga tgtaccttca gacgaaccac ttgttgatat
    22861 gtattttgca cttccccatc caacaacaga actcgatacc cttactttat accatccatt
    22921 agacttttct aaaatctcta cagtttttcc cttgtataat tttgctacct tatcgcaatc
    22981 agaacttggt ccacttctta tattcaaggc acttgctgtt actgttccct tttcaagagc
    23041 acttgcattt atggagctaa cagatactgc tactgcccca attcctaaag ctgctatagc
    23101 ttttttcact actatcactc cccccaacaa atttttatag tcataaattc ataacattat
    23161 tatccatttt tagtccctac aatttaatga taacttattt tcttattaat tgtaaagaaa
    23221 ttataaacat atccttaaaa ttagtgattt ttaggaagta tttcaatcca ataattatct
    23281 gggtccgcta taaaataaat tcccatactt tcattttcaa aacatatgca atctaaatct
    23341 ttatgaagtt tttttgcctc atcaaactcc tcaacttcta aagccagatg aaattcattg
    23401 tctcctaaat tatatggtct atcccaatct ctaagccaag ttaattctaa tgtatgacta
    23461 gtaatgccat ctcctaaata aactaatata aaacttccat cttctgcttc cttgcgcttt
    23521 acctctttaa gccctaaagc ttttttataa aaatctaaac ttttttctaa attagtaaca
    23581 ttgaaattgt tatgacaaaa attaaacttc attttgaaat cactctccaa tttttatttt
    23641 ttttatatta tatcattatt ataaatttat ttaaatcata ttaagttaaa aatttgaatg
    23701 atgatgacca attaccaaat catggtaaat tatttaaaag aatatgtcaa aaaaagaata
    23761 agctctttca aatgcataaa aaatttatac ccaagttata cttaatcatg caaattggca
    23821 gaacttattc tttaaaactc taacttttaa gtttttcttt aataatataa tttattactt
    23881 tttttctaat atcttcagca ttataatcat cttctacttc tatatccaaa agttttgcta
    23941 ttgacctcat atcttctcta gtctttgacc tatagcttga tattaattca tcttttattt
    24001 tctctggttc taaaagatat tcttcatctc ctcttttgaa aacaacgtat ttttgagcat
    24061 atgaacttct attgtagtat atatatcttg atatttttct taaaatttga ttatatgaaa
    24121 gtcgcttgtt aatatcaagt tttaagtact ctgaaaaagc aaatagctct ttttttgatt
    24181 taaataatgc ttctttcttt atagttcttg atattgaagt tacactttct tgatatgcac
    24241 acttttttaa catttttaaa tatactctat taagaccaat actttgattg aattttttta
    24301 tattattatt aataatctta tccaagttac cttcaaagta tttttttgct cctttttgtg
    24361 atacataact ctcaagttct ttgtgggcca tatcaagtat catttttttt aaatcctctt
    24421 tatatcccct agattccatg atactccctc ccttagctat ttatagtata ttcaaaaagc
    24481 tcaagaaatg tgagtcatta caaaattttt ttacttttta tgtatatatt aatttaaaag
    24541 gggagataat agtaatgtaa aatcaaacat actcaatctc ccccattttt agacctctat
    24601 tactccccca atagaggtct ttttttagtt tatcaatttc tattcaattc cctttagttc
    24661 aaatcctaat tctgctatca cttcttttaa tttttcaata gatacatcat ctttcatatc
    24721 tacagatgca cacttatttt ctaaatctac atctaaaact tcaataccat ctatatcttc
    24781 tgttaatgca gttttaagat ggtttacaca atgaccacaa ctcatacctt ctactaatag
    24841 tttcttcttc atttaattac ctccttatac cctataaggg tattctatca tatagtttat
    24901 actttttaaa tagaaattaa tcatttaatt tgattcattc gattttatat ctaaattaga
    24961 tattccctca tatacatcaa aatcatttgg agtaatttta ccttttaatc tactattact
    25021 tacaatatag ttgtttctca cagctaaact tataaatgtt gcattatctt ttatatatgt
    25081 ctgtacatct ttgtatgctt tttttgtttc aatttcagac gtagaacttg taagtgcaga
    25141 catatatcca tttaacttat catcagtata tccactactc gctataatac tacttgcatc
    25201 tggtataatt gatagtttcc atccaactaa tgctaaatca taatttttag aattaagagc
    25261 attttccata tccttatctg acagctgatt tactgtacta tttattccta tagcttttaa
    25321 gttttcattt attatgtttg cagtttttat tctctcacta ttatctctgt ttactattat
    25381 ctttaagttc atttcagaaa gacttttctt tacttcctct ttttctctat tttttttctc
    25441 ttcagcttct tttttgattc tatcttcttc tgctttttta gcttttgctt cttcttctgg
    25501 cgtaagcttc tttgagttct ttttagctga ttttttctga ttttttactt tattgtcatt
    25561 ttgatttact gagtttacat ttgcatattc taccttagca agacactcta tcgccttatc
    25621 tttattaaac tctaaatcct tcatttcact attatagtat tttgattttg aattaagtgg
    25681 aaaattaact ggagtagcat catccatata accttcacta ataattctat ctttatctat
    25741 agagtgtgca attgcttttc taaaattaac atctttgaaa aaaggattat tataattaaa
    25801 taatacacac tcataatctc ttccttgata ttttgttata ttaaactctt tttcttgaaa
    25861 cttacttaaa tcatttaatg taacattggt tatatcacta tcaagagcca taaccataga
    25921 tgtccttgca tcttcattag gcaccatacc tacttttatg ttcttcatgg tttttggaac
    25981 ttcttcatag taatctttgt ttacagataa aaccattcct tctctttctg tatatgattc
    26041 tattttatat ttcccatttc ctatcatatt attattcttg tcatttacat cttttgaatc
    26101 taaatgattt tgagatacaa ttggaaaaat taaactatca attgaaaaag catatctagc
    26161 tttaaatttt attgtaaaat ctttatctga tttaatattc acagaagcta tatttttagt
    26221 aaattcatta tatggactat ctgcactttt ttgaattaaa ccaatagtat agtttacatc
    26281 atttgaagta acattttttc catcatgcca ctttgcatct tttagcttta tatctatact
    26341 cattccatcc tgagctatat tatactcctt aactaattga ggaattacat tgtagttctc
    26401 atctatagta aataaactat cataaactaa attcattata taaccaacag acttatcagt
    26461 attagttata ggatttatag tttttggagt taccattgtt aagtttatat acttagcatc
    26521 tatcgaactt tgactttgta aacttgaatc actactactt tgttttgttt tagtattact
    26581 acaaccagct accataagag ttatagctaa aacgactgtt aagactttta ttctcttcat
    26641 tagttttttc ctttcttgcc atatatctta ttataatgtt cgtaattatt ttttactttt
    26701 tctacatatt ttgaagtttg cttaaaaggt atatcatgta gattttcgcc atctttacta
    26761 tattcattat tttctaacca ttttttcaca ttacctgaac caccattata tgcagctatc
    26821 actaaatcta atttaccaaa ttctttgtat aacttactta aataccaaca gccaagtctt
    26881 atattagtct ctgggtcgaa aatatccaca ttttttaaat ttagttcctc tgctccccaa
    26941 tctctagtta tgtctaatat ttgcattaat ccttttgctt ccttttttga aacagcagaa
    27001 ctattaaact tactttcggc tttaataaca ctgtaaacta tattttcatc taaattaaat
    27061 tctttcgaat acttttctac atactctgaa tattttttag gatataaaaa tttatgtatt
    27121 actttgcttt ccattaatag tgccccaaat aagattataa aaatagatag aatcaatact
    27181 ttcttactat tcacgccaat tctccttcat gtactcaata aatttatgtg ctttactttc
    27241 taattcagtt attgttcccg aattatctat tatataatca ccatatttgg atttttcttc
    27301 ctgactcatc tgtgaattta tcctactaag tgcttcctgc tcactgcaat tatctctctt
    27361 ttgtattcta ctaatttgta cttcttgttt acaagtaact actaatagct tgtcaaccat
    27421 atctaaaaaa ccactttcta ccaatattgc cgcatctaac actactatat tttcgccttt
    27481 ttttctcaat ttttctattt cacttattat tttttctctt attcgtggat gagtcaagtt
    27541 atttaattcc ttaagttttt cttcatcact aaaaactatt ttcccaagag cttttctatt
    27601 tagagttcca tcatcatttt ttatactttg cccaaaatgg acaaatactt tttccaataa
    27661 caacttatcc tcaaatatct tcctggatat aatatcagca tccactatag gaatgttaag
    27721 atttctaaat atatttgata ggctgctttt tccacagcct attcctcctg taagtcctaa
    27781 tatcaacata aaatcaccta ctttgtctca taccaagaac tacctacatt taaatctaca
    27841 tctaagtgaa catccatatt tacagcattt tccatttctt cttttactat tttcttaact
    27901 atatctattt catcatcaat tgcttcaact atcaactcgt cgtgaacttg tagtataagt
    27961 tttgatttca aattgttctc ttccaatttt ttatgaacat ttatcatagc aatttttatt
    28021 atatctgctg cacttccttg tattggtgca ttcattgcaa atctctttcc tctatttctt
    28081 tccataaaat tgcttgactt tatttctggt atatatcttc ttctgtttaa aattgttaca
    28141 gcatatccat cctcactagc cttttctact gttgtgtcca taaactcttt tattttatgg
    28201 tatttagcaa aataactttc gatatattct ttcgcctttg gaacaggtat atttaaatca
    28261 tcagctagac caaagtcact tttaccatat ataataccaa agtttacagc tttagctgca
    28321 cctctcaatt cactagtaac atcttccata ggaacattaa aaacttgtga agctgttttg
    28381 gtatgaatat cttcatgatg tttaaatgct tctatcatat tttcatcctg actcatatgt
    28441 gcaagaactc ttagctctac ttgtgagtaa tcggcatcaa ccaacttaca gttatcatct
    28501 gcaataaaca cttttcttat ttttctaccc atttccattt ttataggtat attttgaaga
    28561 tttggttctg tagatgaaat tcttcctgta gtcgttatag tttgattaaa tgatgaatgt
    28621 attctattac tttctggatt tattatattt aaaagtcctt caacgtaagt agaatttaac
    28681 tttactattt gtctatactc agttatctta tctattattg gatgcttatc tcttaatttt
    28741 tctaaaacat ctgcattagt tgaatatcca gttttagttt ttttaattat tggaagctct
    28801 aatttttcaa ataatataac acccaattgt tttggtgaat ttatattgaa tggctctcct
    28861 gataattcat aaatttcctt ttctaatgta gctattattt ctttaaattc aacactcaat
    28921 tcatccaata tacttttgtc tacttttata ccttcatact ccatatgacc taagacttct
    28981 actaaaggca tctctacatg ataaaaaaga ccatccatac tcatatccat tagacttctt
    29041 tccatcatag gaactgtttc tttaactgtg catattattt gacccatata ctctgctaaa
    29101 tcctcaaatt ctaaatcctc atagttttta gatttaacac cctttcctaa taattcttct
    29161 tttgatttaa ccttttttga taaatacttc atagctattg cactacaatc atatgaggta
    29221 gatgaagttg aatctattaa gtactcagct attgttatat caaaatacat attttctaag
    29281 tttatattat atggttttaa agatatataa tcatctttta aattatatcc atacttttta
    29341 atttcattat ttgaaaatat actatctaac tttgacactt catttgaatc tacataatat
    29401 attttttctc catctacact tataaaaata tatattatat ttttttcaag tatattacct
    29461 tgtcctctaa cagtttttaa aataacttgt ccttttttgt ttacttcatc tataaatttt
    29521 tctatatctt ttaattttaa tatttctatt ttttcttcta cgtgttctgt gtcatctgaa
    29581 tcgaccaaat caactaatct tgataagaga cttgtaaatc caaagtatct aaatttatct
    29641 aaaagttcat ctttatcata atcgccaaaa atcatactat ctaaatccac ttctattggc
    29701 acatctctta taattgttgc aagtctttta ctttgaatag ctatttcttt gttttcctct
    29761 attttctttt ttacactacc tttaagctta tctgtattct ctattagatt ctctatacta
    29821 gaaaactctt ttataagctt tatacctgtt ttttcaccta ttccaggtac tccaggtata
    29881 ttatcagact tgtctcccat aagacccttt aaatcgataa attgtgttgg agtcatctca
    29941 tatttttcaa ttacagaatt aaagttgtat tcttctactt ctccaacccc ttttttcgtt
    30001 ataagggtag ttgtattatc tgaagcaagt tgaatagcat ctttatctcc agttactatg
    30061 tataccttat atccattttc ttctgctttt ttagaaacag tacctataat atcatctgcc
    30121 tcataaccat ctatttctag tctatttatt ctaaatttat ctaaaaggtc ttttaatggc
    30181 tgtaattgtt ctgccaattc atctggcata ccctttcttc cagccttata atctttatat
    30241 tctaaatgcc taaatgtagg tgcttttctg tcaaaagcta cacttatatg tgttggttta
    30301 tatatatcta tcattttaaa tagcattgta gtaaacccat atatagcatt ggtctttaac
    30361 ccttctttat tattcatttc tggaagagca taaaaagctc tatttattat tgaatttcca
    30421 tctattataa ttaatttttt ttccaaatta tatcactcct aaaaattatc tatttttgta
    30481 acatatatat tatataccaa tatagtgtta tttaatattc ccataataat aatagtatat
    30541 taaaattaaa tatgttgcaa acaatatatt tttttgctat aataatatac atgacatgcc
    30601 cgagtggtgg aattggtaga cgcagtggac tcaaaatcca ccggactaat actccgtgcc
    30661 ggttcgagtc cggcctcggg caccattaaa aactcctaaa gctatttatt caatagatta
    30721 ggagtttttt tatttttgat ttaaatttta agttattttt tgaaattact gtgtcttttt
    30781 aaaatttata taattatata aatttatctt ctagcattaa tttaaaaact aatttacttt
    30841 tatataattt tctattttat atcataactt attacaacct tttatctaat ttattgtata
    30901 caacaacact acaaataaat tgaaataaaa taagcaatcc aagaacaact aaaacccact
    30961 tgcttattac cattacagaa aatacactca atatagaaac aataaatata agaattacaa
    31021 gaacatagca catgatttga ttagtaatac gtccagcttt atcacgtaac ataattcttc
    31081 tttcatcatt catctctatc ctttgatttt ttatcttttc ttcataatct actgctcttt
    31141 caggtttact ccaatacata tattgaagaa tcatacatac acctggacct aaagcagctc
    31201 cagataatcc ccaaataaaa gcctcaaatg aaaactcagt aaatgtagcc aataaaacaa
    31261 gtaaaattcc acaaataaaa tatataattc caacatataa catattcttt ttcattttta
    31321 ctcctcctca taaataaata tttcttcaat actcatatca aagtatctag ctatcttaaa
    31381 ggctaagatg atagaaggat tatacctacc attttctaat gaacctatag tttgtcttga
    31441 tacttgcaaa atagaagcaa gttcttcttg ttttatgcct ctttcttttc ttatttcttc
    31501 taatctattt ttcacatcta tcccccttta atatctcaac tacggaaagt ctactttcca
    31561 taattataca actatattcc atttatgtaa agcttacttt ccatatttac aaaaaaataa
    31621 cagactattt tcagcctgta attttagaat ttcatgttgt ttattaattt taatgttatt
    31681 tattaattcc atattattta ttaattttaa cttggtttct gaattggaat ccaaacttct
    31741 gtagtataat caggagcatt gatatcgcca ttgggataaa gttcaatatc tggtaaatta
    31801 gcatacacat acccagaact tggtaaccac tcagtaataa ttcttttttg caattcctgt
    31861 attgccagag catttggcat tggagaaaca cattcaaata ctgcccatgt gcctgatggt
    31921 attaagtatt catgagtatc ttctaataca ggcttatctg ttggagcagc aatataataa
    31981 tctaaaccct tactatttgt acacacacta actccaagca atccacaagg ttcattattt
    32041 attaactctg aaagtttatc aattgttcca ttttttatgc atttagccca caacttagga
    32101 actttctcaa agcactcttc tacaatcatg ttcatacttt cttttatacc aacaatacga
    32161 aaccctttct tttctacaat acgatattgc atttctatat ttccttttac ataaatgctt
    32221 aatgtcatct taggatatgc ttttaaaaca acaccctttg ctctagctgc agatggagaa
    32281 atgttatgta tattttgaaa agctctatta aaagatgttg gtgaatcgta accatacttc
    32341 tcagatagtt ctaaaatttt gatgttactg ttctgaagtt caaatgcagc cttagtcata
    32401 cgtcttcgtc taatatattc agataatggt acttctgcaa tatatgaaaa cattctttga
    32461 aaatgaaagg ttgaacaaca tgcaattttt gctgcttctg cataatccac tccactttcg
    32521 agattttctt caatataagt aatagcttga tttaatttat ttatccattc catttgatat
    32581 tacctccaag caaattatac agtttaataa aataatatac ctctctattc atgcacaaaa
    32641 ttgtaagcat ttaaatttat aagcattttt caatcaatac aataaatata aaaattgtat
    32701 tatacaataa aaaagttcaa taacattata ttgtaaaatg ttattgaact ttttataaaa
    32761 attaatttaa gtgtagttaa attaagtatt acactatctt atatattaca atatcctact
    32821 atcttatagt cttctggcac tttataatct ttgtctggag tacctaaatt ccattttaaa
    32881 tcaaacatag ttgcacttat tattgttgca aaataaagca taactatacc aatgtctatt
    32941 ttttcttcat ataaattagt atgaccatct tttctaacag ctaaaactac agtgcctcca
    33001 tcaactataa atctccatgg ttgtctattt aatgtagatg gagctaatct tgcatagtga
    33061 aatgcatcaa gaagtgctct ttcttctaag atttctatac ttgcaacatt tccccattcg
    33121 ttcatataaa caacatcttc tacacctagt ctagtagacc ccttatctgt tacaacttgc
    33181 atatcagatt ttgaataatt ttgcccagtt tttgtagcat ttaatacttt agcattactt
    33241 acattttctc catatccaat agctataatt cctgttacct ctttatctga attaattcca
    33301 agtttttctt ttaccttttt tccatcttca aaagtaaccc aacaagaacc aattccaagt
    33361 tctgttactt taaagattat actttctcct atgtaccctg aattttcaat gtaattatct
    33421 tttatgtcag ataatattat aatataactt ggagaatcta tcatatttcc tttgtaccct
    33481 gctactttat ctaaattatt aaaaacttca cttctattca taattcttac atcaacatct
    33541 atttctggaa ctagtttctt acaagattct gagtatttct taatttcact aagatattct
    33601 tctttaatat ctgtgttctt atattctcta acagactttc tatttaatat gattgtttta
    33661 taatccataa ttttatcctc cccaaactag tattgtctta attatatacc cattaatata
    33721 ttattttact catttattta ataaataaag attacaattt tgtaattatt atttattaat
    33781 aatattccac ttttacatta ataatgacca gttgtgcaaa atccattgcc aaccaccaaa
    33841 taaaaacata ttataaaggt aatataaaat aacaaacaaa tcttaacaaa aaattaaact
    33901 ttttttacac gaaagaaggc ttcccgttgc cttctttatt tttataaaaa aataagctgt
    33961 ttctaaaatg atttcacatt tttgaaacag ccaagttatt ctataatgac aactataatt
    34021 tgaggatgtg agggagagtt gtcattataa atttagggta attatattat gttttaagct
    34081 agttctttct atatttatat aatacatttt atatatgaat tttatatgag attggtataa
    34141 atgatatatg aaattacata tgtttatatt atattaaatt ttattattac attttccaca
    34201 acaatactca tatgtctcca cattatttgg aatatttaca aatacaaatt ctttacagtt
    34261 ctcacaatac tttaatatag atatagttcc acaagtgtaa cattcaaata ttacacaatc
    34321 ctcattttcc tctactattt caaacttttt actatgttca ccacactctg ggcacttgtg
    34381 attatttaca ttcataaata ctcctcctta ttagtatagt tacatgtcaa aaacttaatt
    34441 aatgtattct atattactta ttatatattc tataataaat catattctaa tataataaaa
    34501 caaattaaat aaaaaaaaaa ccataaattt taaatttatg gattaattct tgtaatgata
    34561 actctatttg aggatgtgag ggagggttat cattacaata gggtattata ttatggtttt
    34621 taagctagtc tttctacacc tcatatgata catatgctat atgaatttta tatgattttt
    34681 acataaatat tttatgaaat ttaaattttt tagtcatttt gaaatttgta aaaagttctc
    34741 taaaaatcca cagcatcata aacagccaaa atttaagtaa aacatcataa aaaaatttgt
    34801 ccaataattt taacaagtaa tctcacctaa ttataattat tgacacaaac ttaaataaac
    34861 aatctataaa tcttctataa atcttaaact ttccccctat tgatacacaa tttctagttt
    34921 gtcaatttta ttttgcattt ctaagaaaat ttcttcaaca gaattgttaa atatattgcc
    34981 taaatagatt cttttatctt tttgaaatga ataagatttt acactaccat cagaagaaat
    35041 atataaatga ctccactctg ctccatattt tgaaaacctc tcctttgtat ctttaaacct
    35101 catatcttct atttttattg ctttttctct gtctcctctt actacagtgt gacccctttt
    35161 tattaaactg gttattttta tatcttcttt taatttaata cgtgtatcat cattttttga
    35221 atatactttc catatcactt tatgacgata attatctaat tcctttatta agcttctatc
    35281 aatagtaaaa ctatctttag ttaccacaat taaatcaaaa tggtctaaca tatattgaaa
    35341 tatttctctc atacttttat tttttaaaat atcctcacca ataacctcta taaatctaca
    35401 gcctttctca tacatgcagt tcaataaact tgtgatttca gaaaaataat catttgtatt
    35461 attatctcca tctatttcta tagtaaagta caaaggagta atgtatgatt tatttcctgt
    35521 tatctcaaat acacttgata gtgtctgtat atcatcttca aacgttaagt acacattctc
    35581 ataaaacata aaaaattcca taactttttc tttcatatta tggatatttc tatatctatt
    35641 ggataatata tttattatat cgtcagtagt atttttccca tcacattcag ccagtataac
    35701 aatttgcttc caacttaagc tatccacatt tccgatactt tttttatatc catcttcttc
    35761 gtcataaata ggaccaatta taatgttttc ttttggccac agatgaaccc tagttccatc
    35821 ttttaatttc ggatatttat tttttacaga ctcataaaat ctacttgtat cacccatgat
    35881 tctttactac cccccatagt ttaaatattc tatgctaaaa atagcacttt atttttatta
    35941 attcaacaat tattcaaaat tggtcattat atatgtttaa atggatttaa catcaacctt
    36001 atttatatgt tattgatgtt tatatatgtt atataaaatc ttatttctca tacttaaatg
    36061 taatcttaac tatctctggt ttacaattaa gcctaattaa aacttttgag cctcctaagc
    36121 ctttatttaa aatcatagtg ctattatctt tttcatattt tccccagtca tacttaggaa
    36181 agaactctct atttggagat aatacacctc caacaaaagg tattcttatt atacctccat
    36241 gaacatgtcc tgctaaaacc aaatctgctc cccattttgc atatttttca aagaaaaatg
    36301 gtgtatgtgc taaaagaatg ttatattcat tttcattaac tttacctaga ttgtcttcta
    36361 caaaattgtc acttagttct aattttttgt tattagcaaa taaatatgga tagcattcca
    36421 atggtataat taaaccatat aaattaagat ggctattacc tctatatatt cttacagatt
    36481 cattttccat atttattatt ttctttgaat atagttcctt aaaataatcc ttatatagat
    36541 gtttatactt ttttattaaa gatttttgtt catgatttcc aattatatga tatacttcat
    36601 atttttcaac caaattatct attaaatcta atgcaacttg aaaattttta ttttctccat
    36661 ctactaaatc tcctgttata aatataacat ctgggtttaa tgattcaatt ttatctatca
    36721 ataccttatt atttttatca aacaccttat tatgtaaatc actcacttga actattttaa
    36781 agttatcaaa ttcctttgga atttttttgt tgaaaatagt atattctgtc gtttctatat
    36841 gctgagtttc atacttatac agaatataca ataatattaa tactaatact gtcattgaaa
    36901 cctttataat catattctct tcctctttat tctattttgt taatattatt ataatgcttt
    36961 tatgtttttt ttaaaacaat aacatcatca catacatata aatcttttcg acattataaa
    37021 aataagttat ccttttctaa aattttcatt tattttagaa aaggataact ataaaattat
    37081 ctataaacct ctatattgtg aaatatagtt tttcctttat ttaaattttt atatatatgc
    37141 tctaaagttc catcaaacct aatagaatgt ccagactcta aaatataagt tttcccatcc
    37201 acattaaggt caagtttacc ttctattacg agtacatact cttctaaaac atgactgtgt
    37261 gtagatgaaa catgagtaca gttttcctca agctcaatta taaaaagttc aaaccttctt
    37321 tgagggctaa aagggtatat tggatataac ttcatgttcc ctttttgctc aacaataggc
    37381 tctacttctt ctttctttat aacctcatag gtatttgtat tttcatttaa tagtgtagta
    37441 aaatttattt taaatccaga tgaaattttc cataaagtag atactgtcgg acttgattca
    37501 cctctctcaa tctgtccaag cattgcttta ctgacacctg tcatctttga agctttgtct
    37561 aaacttatgc ccatttcttg tctcaataac cttaaatttt ctcccacatt aggaaattta
    37621 ttatccatgt taaaatcctc ctgatagcta tttgtattct atgattttta ctcctgtaag
    37681 ttctactatc tcttttaatt ttaacatatg attgttacaa atttcatcaa attcatatat
    37741 ataatcttca agccctaatg ccttatattt atcgattcca aatttatgat atggaagtaa
    37801 ctccatcttg cctcctggaa cattttgttt gacaaataat gcagtatttc ttatattttc
    37861 ttcactatca tttactccat aaataattgg cactcttata accatagatt tattcaactt
    37921 tgaaagccta catatattat ctaatataat tttattactt actcctgtat attctttatg
    37981 aatattatca tccatagatt ttatatctac aaatatgtgg tctattttct caaatacatc
    38041 atttacctta ttccaatcaa aatatccaca agtttcaatt gcaatattta tgcctttatc
    38101 ataaaaaata tcaactaatt ctcttaaaaa atcaatctga agtgtaggtt cacctccaga
    38161 aaaagttatc cctccattag attcaaagaa aaatataaaa tctttttcaa cttcctctac
    38221 tatttcttca acactcattt cttcggtcat tatacaaatg gcatctttaa gacatacctt
    38281 gatacactct ccacataaat cacatttatt atttatttgc gctctatcaa atagtgaact
    38341 tatattttga ggacaaacat ctatacatct attacaggat acacatttat ctttcataac
    38401 acctaatttt actatgttac tccaggaatc tggattagaa caccacttac attttaattt
    38461 acatccttct aaaaatatag tagtccttat tccatctcca tcatttactg aaaaatgttg
    38521 tacttttaca actctacctt ttttactcat aatctacctc ataattaaaa attgctatgt
    38581 gaatttctag ctattatagc atcttgcatt tctcttgata aattaacaaa ttgagtacta
    38641 tatccagcaa ctctcactaa taaatcttta tattcttctg gattttcttg agcttttaat
    38701 agagtatcat ttgatatcgt attaaattgt acatggaatc ctcctaaagc aaaatatgat
    38761 ttaatcatag atgttagatt tctaagtcct ctttcagttt ccaccaaatc ttgattcagt
    38821 cttaaattta gaagtgttcc accagtatga acatcatgat ttatttttga tgcagatttc
    38881 attgctgcta gtggacttgt tgtatcagta ccaacaaatg gagaaactcc ctctgtcaaa
    38941 ggttttgtcg cttttcttcc acaaggagtt gcgcctacga tttttccagt tggtacatag
    39001 tttgatatac ccataaatgc actattaaat ttagagccaa aaatatcctt gtattttcta
    39061 gtttcagtat aataaaaatc agaaacttca attgctaatg aatctacata atcattatca
    39121 tttccatact taggaacatc caacgcaagt tttcttaatt cttcataacc ttcccaatca
    39181 ttatttagtg cttttgttaa ttcttctaac gtagtcacct tatcctcaaa tactaatttt
    39241 tttatagctg ccaatgaatt tgaaactaca cctaacccta taccagttaa tacaggacct
    39301 atattatatt ttgctcctcc tttacttaaa tcaactccct tatccaaaca tccatctaca
    39361 caagtagata aaaatggtct tggtaccatt tctttatgaa tttgttgagc aacagtagtt
    39421 cctataacag aactttttac aagatatgct aattgcttta aaaactcatc cttaagttct
    39481 tcaaaagaag taaattctgt gatatttttt gtatctaacc ccattttttc tccagttaat
    39541 ctactcttac cttcattcat agcatattct aaagctgctg caaaattgat atttacggct
    39601 gaagtccact gtcctgtttt tctaaaatga ggaactacac aaccacagtt actccaatct
    39661 ctagcatctt ctgcatcata tccatcttgt aataacattt gtgcccctgc actatcgctg
    39721 tgtatagcag ggaaaccagt tccttgtttt accaatttag caactgccat tacgaaattg
    39781 tctggtgctc cttgactcac tctaacactt aatcctggtt ggtgagtttt tacactttct
    39841 gtagccttta aacacatata tgagatatca ttcgttccat cagtaccatt tctcttttta
    39901 ccaccaacag taagattttg gaattgatta tatcctgcaa agtaatttgc tgtatttgct
    39961 gatatagtcc atacccattc tgataactta atccaaagtg cttctatcaa ttcttgagct
    40021 tctgattcag ttattttacc ttctctagca tcattttcat aatatggata catatattga
    40081 tcaaatctac ctaaatttaa tgccaatgga ttttctgata gaataccacc taattgaaca
    40141 aaccatacaa attgtattgc ttcataaaaa ttagttggag ggtttgctgg tacattcata
    40201 catatctctg aaatttttat aagttctaac tttctctttt catctgtttc ttgcttactc
    40261 atctcttttg caagatttga atatctttgt gccaatgtca taataccctg tgcaactata
    40321 gaaacagatt tgtaaaaatc tatcttttca atattttctg aaactgatat atctaattct
    40381 ttaatttttt tatctgcatc ttccttaatc cctttatatc cttttacaaa caatatatct
    40441 tgataatctg gtgttacttc accaactgcc tgtctccact tagaatcatt atctattatt
    40501 cctgtatcta ctaatatctt agctgtatct tctgggattc ttgctaagaa aacttcttcc
    40561 aaagattttc ccttccaata tggaaatatt tcttttctta taaattctat ttgttctttt
    40621 gatattacat atgggtcttg agttctttta tcaaaagtat ccatttcttt gtctacccat
    40681 tgccaagaaa actctggtgt caatatccct gttcttctaa attttcctgc tgtacctact
    40741 attaattcat tttcaaagat ttttatagga agttctttac atgtctccaa aaaagcttta
    40801 gctcttctta tacagatagc ttctccctca gtttttttat gtgattctgt aaaaattcta
    40861 gccctttcac aacatatcat aggtttagaa ttaatatatt cttgtctaaa tgcttctatt
    40921 ctattagttt gactcattta aaatccccct ttggataata tatactacat ttttgtatgt
    40981 tataacatac tttaattata ttatactgtc ctttagcata aaatggaagc aaaataactt
    41041 attttttttc atctaccttg aacacctata tattaatact ttttttgtta aatttataaa
    41101 atctaaaaaa attttctatg atatattttc tatattgtta tagttatgtc taatatacta
    41161 atgatttgaa atatcaaatt tgtatactag acttagtttt aaaaccttag ttgattgttt
    41221 aactttgtta acttcaacat gtatatcctt catataagga agctctacaa cacctactcc
    41281 cttattaaag tcttcttcaa ccatagaaaa aatttctttc tcagttaagt tcgtatcagt
    41341 caatgattta aataaattgt atataaattt aacatattta tcactctcag ataacttatc
    41401 atcttctgga tactctttag cacagagaat atctattgtt acttttcctt ctttatcgtc
    41461 ttcagacata gaatagaata aacttcgaat atttttttca ggtggttttt cagcaccatt
    41521 tatagatact acaaagctac catctgattt tgagttagta atactataac cactgtctat
    41581 tatagctttc gaaaatcttt gagataaatc tttttttact tcttctctac ctttcgcatc
    41641 attaatcttt tgtgagtaat tattaaatag ctcaatagat tcatcttttt tatcttcaac
    41701 ttgttctttc aatgaatctt tactaatttg ttctgagcta cttaagttct tatcagatac
    41761 aggagacata caccctacca tcaatgctaa gctaagattt aaagccacaa ttattgccac
    41821 tctatttata ttccttttta ctttcataca caatcctccc atcacactaa ccgctctaaa
    41881 gtattattca ttgcactaga atatatttat attttcttta tattaaagat ttattattca
    41941 atacatatca ccttcctatc caattcttat aacttactat tagtaaaatt ataagctcct
    42001 taatacagaa ataaggatgt tatcttttgt tattaaaaga taacatcctt atttttttgt
    42061 atcaatacat atgtcccaac tggtaactta atcgccccaa ctgtcagtat cttggtccaa
    42121 tggtatatat atatttagta taaatttatc gatactattt tcaaataata attctccatt
    42181 atatttttgt aaagaagatt taatactctg aatacctatt ccatgtacga acttatccat
    42241 tttgtttgtt aacaatatat ttttattaaa agtaagctga tttagtttac tattttcaca
    42301 tttaagtaca aaaaatgacc ttgttatagt tcctttaatt ctaatatatt tattaatatt
    42361 ttcatccact attttatcac atgcttctat tgcattatcc aaaatattag aaaatatgct
    42421 tgacacatct ataggtttta caaaatttac ttttgaaaaa tttatagagc aagtaaattt
    42481 aattcctttt tttaaacata cttcactctt ttcgttgatt ataatgtcta atatcatatt
    42541 tcctgtatta tagatatatt taaatgtttt tatttcatct tttaaattgt taacatattc
    42601 attaatttct ttgctattgt ttctaaggtt gtctatacaa taaatgtgat tatttatatc
    42661 gtgatatagt ttcttaactc gcatatgaga ttcttgaata cttaaataat gcgcatattg
    42721 catatctaac ttatttttta ttaattcagc ttgagatttt aatctattct cttttattat
    42781 ttctttaaac aatagtataa gaaatatact actagctcct accaattttg gattaacaaa
    42841 attatcaaaa tagtatcctt ctgaatgtaa tgacccaaac cgttctaatt tttttaatac
    42901 ataatttaca agcatattca ttgtattaac taataaggca aataataaat atacataatt
    42961 tctcttatct tggctaatta attttataga ctttctaaaa aagctaaata taagatatat
    43021 aagaataatt attaaattac tatatatctg aatttttata gattctaagt aaggtactaa
    43081 taaagattct ttagatgaaa taacattacc tgtaattgca aaaaatagta ttctatatat
    43141 aacagcatcc aaacatttat aatatacact aaatagataa aagaaaatgg aatacttaat
    43201 ataacccatc ttataaaaat aattatagta tcctccccaa gcaataaatg ctaatatata
    43261 aaataaatag tttatattac taaagtaatt tcctccatta aataatctac ttatctgaat
    43321 tataatataa ataagaaaca agacaatata gaatactatt ttttcatttt ttttactatc
    43381 ctttttataa attaaaattc cgttcattat agaaaactgt ataaatatag ataaaactac
    43441 taatagctca gtcataaagt ttagcatatt gtatcaccaa gaaattttaa gaatttctcc
    43501 ttaacttctt tatatctata tctactaata ggtacctctt ctccactttc cagtattgca
    43561 gtattaggct ttatattttc aacatatctt aaatttacta taaaactttt atggcatctt
    43621 ataaatttat atggatttaa ttctttttct attttgccta aactatattt tatatcaaaa
    43681 ttcttgttta ttgtgtgtat tagcatatct tttttttgaa cttctatata ttttatttca
    43741 tttaaataaa tcttatatgt attagactta ttttttattg taatatgact ttccttattt
    43801 atttcaatat ctttgataca agttagcaca tgttttttta gttcttccaa ctctattggc
    43861 tttagtaaat acctataagc tcttacctca taaccttctt gcacataatc tattaatgaa
    43921 gttacaaata ttatttccat ttcattgtct acttctctaa tctttcttgc agtatccatt
    43981 ccatttaatt tttccatttt tatatcaaga agtaatatat ctatatcttt taaattactt
    44041 tccagtaact cttctccaga tatatacttt tgtatctcat attctatact tatatctttt
    44101 aatatattct caaggtaatc ttctattaaa agttgtgttt ctttttcatc ttcacaaact
    44161 gctatcttaa tcataaaatg cctccttaca taatttctag ttaatacaat taaaatattt
    44221 ataatatatt taaagataat tttgtatatc tgatacttta ggataacatt tttaaattat
    44281 ttttatcaat actcaatccc atacaactat tatttgatat ttcaatttaa agacagaaat
    44341 tactataatt aaaaacagta cttactgaca aaagaagatg tcttaaaata ttaaaaaaat
    44401 aattttagga caccttcttt tatttattta aactttttaa gccttaaata atacattaat
    44461 ctcctatata attgtactgc atataaaatc aacaatataa tctgtagtac aatagcagtt
    44521 acagatgctc cataaccact atataaattt cttatttcaa tattcaataa atatataaat
    44581 attactacta acataaagaa tataaacata ttcataaata aaattgactt tactttattt
    44641 gttttatcag aatcactata taatttagga ggtatgtctt tgtattcttt cgaccatata
    44701 taccatgcag atgtacccat aaaactacca atactcctaa atacaagatt ccagccattt
    44761 gatttatgtt catcaaaata attattctct acatcatagt agtaatcaac agtgtatttt
    44821 atttttcttg gacttccctt atgaaaataa aatattcctc caaatttatt tatcttatat
    44881 aaattatatc ctttagcttc aatatcttct aaccatttct ctatcttatc aggtgaacta
    44941 taccagtcaa atttgaattt tattttcttt tccatagcac tcattctcca aaatgatatt
    45001 tttaaatagc ttaaaaatct atttaaataa ttatatcatt ttgatactaa ttttaggtta
    45061 caatcatata tttaaaagtt atttaaatag taataaacta atagccataa cacccattcc
    45121 tgcaatcaat ccataaatag ataaatgatg ttccccgtac tctctagctg atggcaataa
    45181 ttcatctagt gatatgaata ccataattcc tcctacaatt gcaaatacaa ttccaagagt
    45241 tatatcattt aaaaaatttc taagaagtac atatccaata atcgctccta aaggctcaga
    45301 cataccagat aaaaaggaat aataaaaagc tttcttttta tctcctgtgg cataataaac
    45361 tggtacagat acagatatac cttctggtat attgtgtatt gcaattgcaa ttgctatcgg
    45421 aatagctatt gtaacatcat caagagctga tataaatgta gctaatcctt caggaaaatt
    45481 gtgaattgct atagccattg ctgtaaatat acctgttctt aaaagagact tatttttttt
    45541 gatattcttt tcttctatat tctccatttt ctttatttca tgtggatttt cttcttgtgg
    45601 aattagctta tcaataacag caatgatagc catacctata aaaaaagcta ttacagttga
    45661 ccaacttcct aatttctctc ccatagattt tgtcaaagca tcttgtgctt taggaaaaat
    45721 ttctatcata gaaacataaa tcataacacc agcagaaaat cctaagctta tagaaagaaa
    45781 cttagtattt gtttttttag caaaaaatgc tatacaactt cctataccag tagataaacc
    45841 agccaataat gttatcaaaa atgcaaatat tacattattc aaataaatca ccttcttttt
    45901 aattgatatt gattatcatt ttctttatgc tatcatatat attttatata gtcaatattt
    45961 ataaaaaata tacacaataa aaaagcagag atataaactt agtctctgct ttttctttat
    46021 agtatattaa aattatttat ttaagaaatc cactgcatat tgcacatata aagcagttcc
    46081 aatttctaga gcatcttcat ctatattaaa gcatccatga tgatgaggat aacaagcacc
    46141 ttttgactca tttctagctc ctacaaatgc tagtgcacca ggtgctatat tcataaactc
    46201 agctaaatct tctgctcctg ttactttttc aaatagtgtt atacagtctt ctccaaatag
    46261 cttaattgct gcatctgtag ctatctttga acattctttg tcatttataa ctgctggtgt
    46321 taagtaccca tactctaatt cagcatctgc tctatatgct tctgctgtag attttgctat
    46381 acgttctaaa attctaggaa tttgctttct aagctctgga ttaaatagcc ttattgtacc
    46441 ttctaagata gcttcactcg caattacatt aaatctagtt ccagaattta aaactccaac
    46501 actcacaact aatggttcca gaggacttac ttctctacta accatagatt gtagattcat
    46561 aactattgct gaacttgcaa gcactgcatc aacaccttga tgaggaagtg aaccatgacc
    46621 acctctacct tttacagtaa ttttaaagaa atcagcagaa gccattcttg gtccttcttc
    46681 taccgatatg gtcccagatt ctacatcagt ccaaagatgt attccaaaca cactatctac
    46741 accttccata gcaccatctt gaatcatagc tcttgcacct ttaccaactt cttctcctgg
    46801 ctgaaaaaat aattttacag ttccattgat actatcctta atatcattta acacttttgc
    46861 tgcccctaga agcattgatg tgtgaccatc atgaccacaa gcatgcataa gtccttcatt
    46921 tttagatttg tattctacat cagtgcattc aacaacttgt agagcatcca tatcacccct
    46981 aagtgctaca gtctttcctg gattagctcc ttttatagtt gcaattacac ctgtacctcc
    47041 agctgataca tatggtatcc ctattttatc aagttcttct ttaactctct ttgatgttct
    47101 aacttcttcc atacttttct caggattttc atgaaattcc cttctaagct taattacata
    47161 gtctttataa ctctttgtta tttctttaat atccattcca tacctccaag tagtacacat
    47221 tcttcttatt taaaatactt tttatcactc aaatactata ttagatttac aaagataccc
    47281 gctattacta cagaagctat tgtaactgta gtaaatcctc caacaagcat ctttggaagc
    47341 atcttatcca ttaaaaattc tttttcttct ggtgtttcag ccaaagcctt tactgcctct
    47401 tcagttaata tatagtttgg tggaaaacca taaagtgatg taagcgaaac tgaaaaagcc
    47461 atttctttac tttcacctaa aagcttacct actataatag agaatattcc catacctatt
    47521 acaccaaata ttatgattcc tacaagagga actgccattt caacaagcat tgctggtgta
    47581 gcttttgcta accctgcaaa tataaaagcc atcaatgatg tcattaacca tccaaatgaa
    47641 ccagatttat taagtggttt tctatcaact attccaagtt ctgatgctat tacaccaaat
    47701 attaaacata taacatatct tgatacaact tcatttgtaa gatttgtaaa ttgtactgct
    47761 aaccatgcta caaatcctaa tctaagtatt atcatatatg tcgtatcata tttttctggt
    47821 aagtctggaa ttatcttaaa tctgcttttt tgttcaggta aatccttaac tttttcttca
    47881 tctgtattta cagttacttt tccacttcta aagtctgata aaagtctttt tccttctttt
    47941 ttaagcatta atgctgtaat aggataaccc gcaaatcctt gtgcaacata catcaaaatt
    48001 gcaagtactg ataaacttgc aagacctttt gctgtagcag catctgccat cattatagat
    48061 gcaactagcc ctccagtaag aggaggtgta gctattactg ctgtatccca tccaaatact
    48121 actgtcccta atgccaaagc acctacacat attcctacta ttcctgccaa ggctattact
    48181 attgttttcc attgacctgc taattcctta atactcatca tagttcccat atgagttatt
    48241 aacaaaagca tacctaattg agctaatggt gttcctaaag cggctaagtc cacaatattt
    48301 tctgggaaaa aagtccaaaa tcctactata aatataattg ctgatacaaa aaccgacgga
    48361 acaaaagctt tagtttttgc tgaaactata tctcctatcg tcagtgatac taaaactaga
    48421 caaaaagctg ttattgcctc catacaaaat cccccctcat aataaatata tttttataaa
    48481 atgtatgtat agttatccat tattttacta cttttgaaaa acatttcaag tcaaaattcg
    48541 atttttttca taaaattcga taaattccat aaattaaagt tagttttcta aattattctt
    48601 tttgtaaaaa cgttatcccg accattggct atttaactct attcttacaa agagaattaa
    48661 atgtaatttg caaaaaataa aacataggat acattttatt cataaaacat cttataaata
    48721 taaaatgaat tgtctttatg aatcttaata tatcctatgt ttgtaagttt aatgtgtatg
    48781 ttagctatgg tgtaaaattt gtatactagt tattgtgtaa cctactatat actccatttt
    48841 tgtttactag ctcttcatga gtacctcttt cttcaattcc tttatctgtc aatactatga
    48901 tttcatcagc attttttata gtagtcaatc tatgtgcaac aactaaattt gttctatctt
    48961 tacttaattc ctctaaagac tcttgaattt ctctttctgt tacattgtct aaagcagatg
    49021 tagcctcatc caatattatt attggaggat tctttaaaaa gattcttgaa attgaaattc
    49081 tttgtttttg cccacctgat aattttacgc ctctttctcc tatataagta tcatatccat
    49141 ctggcaaagt ctctacaaag tcatgtattc tagctttttt agcagcagct atcatttctt
    49201 catctgtagc attagggttc ccacaaagaa tattatctct tatagttcct gtaaataaaa
    49261 atacatcttg ttgaactata cctatatttt ttctcaatga ttttaaacta acatctttta
    49321 tactctttcc atcaattttt atatcaccct tatcaaattc ataaaatctt ggcaatagat
    49381 tacataatgt agtctttcct cctccagaag gtccaactaa tgctatagtc ttaccagctt
    49441 ttatagataa acttaaatta tccaaaactt gagtcttatc ttcatatgtg aaagatatat
    49501 tttctatctc aatgtcacct tttacatttt caagttcaat aggattttta gcttcttttt
    49561 gatggtcttg ttccattatc tctatgaatc ttttaaaacc tgtcatacca ttttgatatt
    49621 gctctgtaaa gtttattaat tttttcatag gttgtataaa catttttaca tatagtatat
    49681 aagcagcaaa atcacccaaa gtaatctttc ctatataagt aaaataacca ccagctatta
    49741 caactaccca ctctaagata tccatgtata aattcatacc agagtaatat tctgccatta
    49801 ccttataaga atcttgtctt gctcttctaa ataatttatt gcttcttaca aacttattta
    49861 actcttcttt ctcagtacaa aaagacttag ttaccttcat acctgctata ctattttcta
    49921 aagtagcatt tacatcacct atagtaactc tactcttcat aaaagcaata ttcatcttat
    49981 cttttttagc tattgcaaac caaattataa acggtaatat agcaaatatt ataagcgtaa
    50041 gaggaatatt tatatctata agtataaaga atgaaccaac caacattact atagaaatga
    50101 ataaatcttc aggcccatga tgtgcaagtt ctgttatatc cattaaatca tttactattc
    50161 tagacatagt tactccagat ttgttattat caaaatacgt atttggaagt ctttgtaagt
    50221 gtgtaaatac ctcatttctc atatcagctt gcatatctac accaataaca tgtccccaat
    50281 actgcataaa atagttaagc cctgcttttg ctataaatat aagtataagt gctactgcaa
    50341 aaactactaa cattcttaag tttttatttg gaacaacgtc atccattata tttctagtta
    50401 tcataggata aactaaatca cataaggaaa atagaaaagc tgctataagg tctagtgtaa
    50461 aaattttctt atatggctta taatatttga caaattgttt tatcataagt attaaatccc
    50521 tcctttatta gtacttctat tagtacttct aaaaaatacc cataaattag tataacataa
    50581 caataacttt aaatctttta taaacttaca aagtaataac aatctttttc ataaaattga
    50641 agatttatat catatagttg tatacttgaa acaacataaa aagagctata tctctatatt
    50701 taaaatttta taaagataca actctatatt taatactaaa ttttttttat aaatacaaaa
    50761 ctacttcaat gcatttaata tctttttatc taatgctgtt gtatttatac ctgttatata
    50821 tcctgttgcc tttattgtag gtatatcata agttgttggt agtatagtag tagatacgat
    50881 taaatcagca ccttcttgtt ttgttgaaca ttcagcaatt ttacattgct caacttctgc
    50941 ttgtatatta ttttctttaa ccaatctttc tactctgtca caaactattg ttgatgttgc
    51001 gattcctgct ccacaggcta ctaatatttt tttcatatat tgtcctccta caatttcttt
    51061 aatattttat aactcaaaat aaaattgata aatttcttat atctttattt atataaataa
    51121 ttatatcatt ttatataaga agaacatctt aaaattattc ttttaatatg ttttttattt
    51181 caacattact tatacttaaa atttcatttt ttatacaaga ctctatgtac tttgatatat
    51241 ttttataatc atttaaatta aactgttcaa tattcaattt atattctata tcactgacaa
    51301 tacctatcaa atcagactta ttagataaaa tatcctcata cttacctttt ctagttactt
    51361 ccaatctttt aaatctataa tttttatatc cttctatgat aatcaagtcc atatcttcat
    51421 ataattgaat cattttatat aatggattct tctcttttag ttcttcaata tatgctactc
    51481 gtttatctga tattatacag acttttctcg ccccagattt tctatgttta taagtatctt
    51541 ttccttcctc atccatatca aaatcatgac aggtatgttt aataactcca acacgatacc
    51601 cttttttatt taattctttt attatgcctt caatcaacgt agttttacct actcctgaat
    51661 atgagacaac tgacaatata ttatacataa aattgtgtcc tttcataaaa ctatctaata
    51721 acaacatcca ctaattgatt ttcttttatt gtctcattct ttttaatctt aatcatacaa
    51781 ttacttctta gagttgcaga tagtctagct tttgcatctt tcttatcatt tagctctaca
    51841 tacaatttac cttcacttgt atatagtttt ccacttacaa atctatcttt atttgaaagt
    51901 ttattaaatt ctcctttaaa tatagctttt atatgatgtg ttcccttctt atatgttaaa
    51961 tactcaataa caggaactat cacattatca aataccacta ttgctgcatg tggattacca
    52021 gaaagaccaa ataatagctt ttctcccaat gtacctacat aacaagctcc accaggttta
    52081 atgtttatct tccaaaataa ttctgttgcc cctatttttt tgaatacatc tggaattaaa
    52141 tctttatctc caacagaaac accacctgtg gtaattaaaa aatctacact ctgtaacata
    52201 ttctttacct ttttttctaa atccaatata tcatcagaac aattgcatat tgtatctgct
    52261 tgtattccaa gttttaataa tcttgaatat aaggtatatc tattactatc gtatatttta
    52321 ccattctcta aagtctcatt tatatcttgt agttctgtac ctgtattcaa aattccaact
    52381 tttaaatcat cataaacagt tactttggat attccaagac ttgctaatac tccaatacca
    52441 tcatatccta tattctgatt tttattaata atcaattgcc ccttttttat atcctcaccc
    52501 ttaaatatgt aattatcgta ttcctttaat tttatattta aagttatcat atcacctata
    52561 actgttacat cttctttttt tattacacaa tttgcaccct gtggtatctt tccaccagtc
    52621 attatcttca ctgcttcatt tttatttaat accctattaa atacatctcc tgcatacaaa
    52681 gtccctataa cctttaaatt tctattttca gaatttgcat tatatgcata accatcatag
    52741 ggtgacctat caaatggagg actatcaatt gttgaatata tatcttctgc aactacctta
    52801 tttactgcat ctataagatt tactatcttg accctgttta ttggtaaaac atttttttca
    52861 ataattttta tggcatcttc taattctatc attttaaata ctccccaata tattttttat
    52921 ttaagatatt tctatgtaat atattataga ttataaaagt tggaaatact tttatcttat
    52981 atttaattat aactaatagt tatctataag tgcagttcaa attttataaa tctaataaaa
    53041 aaactgttcc cagctagaaa tttccttcta tcttgaaaca gcatcatatg ctttagaata
    53101 ttttattcta ataaagttcc ttttattatt tttacaattt tatcactttc aatagttttt
    53161 atagatttta ttttgttatc cacaatactt attgtaagta cctctttttt taatattttg
    53221 ttatcttctt tcaatctata actcttaact actttactgc cttcatgatt atatgctgtt
    53281 aaatcataat tcgcatctcc tacaatttca tttattctgg aatcttcaac accaacatac
    53341 tctcccaggt caatatgtct aaaattttct agtgatacag ccgatttttc atggtcactt
    53401 ttttcaatca ctatactaga ttcagcatca atgactgata taccttgtga tgagaaagaa
    53461 ttggtttcaa cattgacaac tttattttcg ttaatatcaa catataaagc acttccatct
    53521 agttcatcat ttccagtata ttttggataa actaatgtca tataacttga tgtatttata
    53581 tcttgcattg tatttgattc ttttaaaact ttaggtttta tataataaaa tgtattataa
    53641 ggtataccca tattctttat aatgtactta taatctttat tcataatttc attaacatca
    53701 ttttgaactt ttgtcatagt tttttgctgt gttgctttac ttgctacttg atttttctct
    53761 ttagtatttt ctttatgaca tccaacaagt ataataccta aaatagttac acaaactaat
    53821 gaccttttta acttcataaa tcaatcctcc tttcttaaat tttatagtat ttatctatat
    53881 ctcaatttac agttatctct tttttcaata attatattta ttatattata aaataattat
    53941 ataatattta tttgaattta ataagattaa gaagctaatt gtaatttttc tgtaataaaa
    54001 aacactttaa agttgaattt tgaaaattca atttacgata tttttattgt aagtacctta
    54061 caacattata ccaaattatt cctaaacaat caaaaactcc atgaatattt aaaggttttc
    54121 tattttaaaa atcacctttg actattaatt tatcaaatat gttataatag ttttagataa
    54181 tttaaggagg attaaataat ggattttaaa caacttgaag tatttgttgc tgttgcaaaa
    54241 catcagagtt tttcaaaggc tgcaagagaa ctttttttaa ctcaacctac tgtaagcgca
    54301 cacatacaga atttagagag agagctagaa actgttctta taaatagaag caacaaagtt
    54361 attacactca caaaatctgg tgaaattctt tatgaacatg ctatatatat attgaataat
    54421 tgcaaacgtg caatttatga tataaaagaa tattcaggaa aaatagaagg tatcatagat
    54481 atagcatgta gttccatacc tgaaacttat attttacctg attttatgaa gagtttttct
    54541 atgagttatc cagatgtaaa attttccata agccattatg attctcaata tgctatatca
    54601 gaaattttaa atgaacgaat aagctttgga ttagttggtt ctaaaatcaa taatcctcaa
    54661 atagagtacc tagacttatt agatgatgaa cttgttctta tcactccttc agattttaaa
    54721 attgataata aaaataattg tatcgatata ggagaattgg cttatctaaa ttttattatg
    54781 agaaaagaag ggtcaggtac aagaaactta atactaaaca cacttagtaa aaataacttt
    54841 ccagtaagta aattaaatgt aatcgctcat gtagaatcaa atgaggctat taaggaaatg
    54901 gttagattag gtcttggagt atcctttata tcgtacattt cagctatcga ttatctcaat
    54961 gcaggtaaaa taaaatgtta taaaataaag gatgttgatt ttactagaaa gtttttcttc
    55021 atttactcta aaaagaaaac tttttcccca ttagaagata aatttctaaa cagattatgt
    55081 gagtatttcg aaattataat ataataactt taataaagta gtgcttaatt ttgagtttat
    55141 aactaattaa taagctaatt ataactatca cataaatgct tataaattat tatgaaccat
    55201 ctttcttttt tatttaattt agtcaaaaaa tccctcgttc aaatgaactt gggatttttt
    55261 tatttatact actccaatag cagccaatac tatacaaata atcataactg aaattaatat
    55321 cgtagttgtc tttacctttt ttcctaataa ccaatacata ataccgaata tcatagctgg
    55381 aagcatacat ggcattattt ggtctaagta agtttgaata ggctctgccc attctccact
    55441 acctacatta acactaagtt ttaacattac attagatgca gtcatacctc caattaccat
    55501 aagccctaac attgaagctg cttccataac ttttgaaact atacttgaac cactcacatc
    55561 tttaaagaat tgagttccat acttaaatcc tttatctaga catatatagc gtataataaa
    55621 atgaggaaca tttattatta aaaggaataa aatcggtccc attatgttac cctgagaagc
    55681 taaagctata ccaaccccag ctgctataag ctttaatgtt ccccagaaaa atgaatctcc
    55741 tatcccagaa actggaccca ttaaacttga tttaacagca tttatactat tagcatcaaa
    55801 ttctttattc tttgcatttt cttcttccat agctccagat atacctacca aaagagtact
    55861 tatatgagga gtaactgaca taaattctaa atgtcttttt aaagccttag atttcttttc
    55921 tttatcatct ttatataatc tacggattat tggtgccatc atgtatgcat atgctatatt
    55981 ttgcatacgt tcgtagttcc aagatgaatc taatgtcaat gaccttctaa aacatttcca
    56041 aaagtctttc ctcgttattt tactatctgg tgaaattgtc tctaatttct tagaactcgt
    56101 tgtcatctac tattacctcc ttctctccta caaagttaac atatattaaa gctattgcta
    56161 atccaaacaa tgcaactcct gttactgtta tgtttaggta agcaaccaat ataaatccta
    56221 ataataaaaa tgctactgtt ttcttattaa gcatcattct agctaacatg gcaaaaccta
    56281 ttgctggaat cacgcctgct gcaacatcca ttccatgagt tacatatgat ggtataaatg
    56341 aaagtatttt ttctattact ggaacaccta atgaaaatcc tatacctact attattgcta
    56401 atattaattt atttcctata ccaaataata attgcacacg ttctacgcct tcaatattcc
    56461 cctcttctgc ataacggtca gcaatatgag ctccccatgt aagtactact gtcattaaga
    56521 agttatctat aagcaatact agagttgcta taggaactgc aagtacaact gctgctgata
    56581 catcttgacc cgttaatatt acaaacgctg tggcaataat agaaccagac acaaaatcag
    56641 gaggattact tgccccaaca ggaaatgcac ccataaatac taattctaat gttgaaccta
    56701 ttactatacc tgtatgaaga ttacctagtg ctaaaccaac aagtggtgca acaactatag
    56761 gtctattatg cattgtagaa cctacaaaac tatgcatata tgcaaagaat gcaattagag
    56821 ttatcaaaat aacttgtgta aattccattt tcatccccct aaaattatat taatttcatt
    56881 acatttgtag catcatcatc aggaactaat tgtacaaata catttacgcc ttctgaatct
    56941 aactctttaa tcatctttat atcatctttt gatacatgca ctgctttaga tatattttca
    57001 cgattatcat ctgattttgt tccaccaagg tttatagatt taatagcttt tacctcttta
    57061 gcaagtctat aaacatcttc tacagattca caaagtatca ataagttgta tttatcagta
    57121 acacctgagt taagtgcttt tatagaatct gctatgcttt tcattactaa cttaacatta
    57181 gatggtttag ccattttaag tcctgccatt ctcaattcat cttttaataa gtcatcactt
    57241 gctatcaata tacagtctgt acttaaaaac tttgtccatg taaatgctac ttgtccatgt
    57301 ataagtctgt ggtctactct tactaattta atcattataa ttcctcccta atttttttga
    57361 tttcatccat gaatgttttt acgtgttcta cactaacatc cccaaaatct ttgtaattat
    57421 ctttaaagta acttcctatt attgccatat ctcctaattt aagctgtttt tttgcatttt
    57481 ccaatgtaac tccagcagct ataaccaatg ggaagtctcc taaattttta cgaaacagtt
    57541 ctattttttc cattgatgtt tcttgacctg tagcattttc tgtaactgct atagcatcac
    57601 atcttgtcat tcctattttt aaatcttctt caacatcatt ttctgataat actggctgat
    57661 atttaaaacg tacgcctcca attaaatacg ctgggcattt tgaacgttgt aatttaaaaa
    57721 actcttctaa agtagcttca tctctaggtt taacatgacc tactacagaa tcaacctgta
    57781 tataactagc attatatttt gttgccagtt caaatcccat agtatcaaca tttaaacagt
    57841 ttactccata tggtattgat aaattagctt ttgatacata ttcaagtatt ctttctaaat
    57901 cataataatt accatagtaa ttttctaaca ttattccatc tacaccattt tcttcaaaga
    57961 ttgtaatctc ttttttagct ctttcaaaga tatcttctgg agtatctcct tttaagtgta
    58021 tcattgctat aattggtttt ttacttttaa atactgataa tattcccttt tttttcataa
    58081 actaaaatac ctcttcttct tctttaattt gtaatgtcac gctttgtatt cctcttctag
    58141 attcttccac taattggtct atatcttcaa ctgttaattc atcagaaaac cttgtaagaa
    58201 gttctaatac catattcaaa ttcattccag aaaataacct aacattatgt agattagtta
    58261 atcttaacat tgctgtacat acacttgctc catataagtc agataaaatt atatattcac
    58321 attctttatc ttctgatatt gttttttcta attctaaagc aaaatctgaa gcactttgtc
    58381 cagggtacaa accatacgaa ctgacacaat ctgctaaatc tcctactatc atttttactg
    58441 catttaaaag cccttttgaa aagtctccat gagatgctaa tattatcttt ttcatactca
    58501 tcctcctact aaattataga ttatatttct gctgatactg catatttgta cttatcactt
    58561 cttataaatt gctcattgta atataagaca tcattatttt ctgtgtacac cgattgtctt
    58621 actttcaata gtggcatatt acttgcacat ttaagatggt cagaatattc tttgtttgaa
    58681 tgttttactt caaatatatt ttctactcgt ttaggtaatg tgcttatatt attagatata
    58741 acctccatta aagatttatc ttctaattct tcttttaata aaaacatgta atctattcta
    58801 aaatagtcaa cttcaaaaat aacaggtact tcatctatta atctaagtct tttaatacat
    58861 attaccttct cttctagttc aatacttaaa gcttcagcta cttgcttatc tgcttttttt
    58921 attaaaacag agattatttt ggtacttggt tttgcattaa tacgatgaca agaattagta
    58981 aaactatttc ctgcacatgt tgcttctatt ctctctggta ttgttacata agtcccttta
    59041 ccacgttttt tacataatat atcttctttt acaagttcat taagtgcatt tctcacagtt
    59101 atacgactta tattaaattc tttacataag tctccttcag atggaagttt atctcctggt
    59161 ttatatatgc cagatgctat ttgttttttt atgttgtcat atagttgttg atataaagga
    59221 acaaaggaat ttacttctaa atccatttct tttttttgtc tcttttcatc cattttgctc
    59281 acctccctat gtaacatttt tataacatta tataacaagt tctttattac atattataac
    59341 aagatgaaat cgttgtcaat ttttttttaa ataattttta atttgacagt attttctttt
    59401 attttctcta gtgtgacaaa ttgctatttt tacataaaaa taagactgtt gtacaacaga
    59461 ttaaattgtt tatacaacag cctttattca cttattacat ttatattttt acttataaat
    59521 ttatattaaa aaatgcctta tatcccttat acatttcata tatgtctact ttagagacaa
    59581 tttcatgtgg tgcatgcata tttaaaactc caacaccaca gtctataacc tctgcacctt
    59641 gatttgccaa tatatgggct atagttccgc ctcctccttg gtctactttt ccaagttcag
    59701 cagtttgcca tactacattt cctttattaa aaattctctt tacttctgac ataaattcag
    59761 cattagcatc attacatcca ctctttcctc ttgaacctgt atacttagtt aaaactactc
    59821 catttcccat atatgccgaa tttctctttt catatgcttc tccaaaattt gggtcatatc
    59881 cagcagaaac atcagctgat aataccttac tatttgctaa agctcttctt actttaatat
    59941 cacaataatc tccttctaaa gcaatcagtt cagccacagt attttcaaaa aactttgagt
    60001 gcatacctgt attcccttta gaaccaattt cttctttatc tacacataat gcaactgaag
    60061 taaattctgg ttgttcagtt tctagtatag cttttacagc agcatatgaa cataccctat
    60121 catcatgccc atatcccaac accatagacc tatcaagccc taaatctcta gccttaccag
    60181 ctggaactac ttcaatttca gcacttaata aatcttcttc aactatattg tatttctcat
    60241 ttaaaatctt taatatattt gctgttatag gttctttttc ttcatcttct aaaggaatat
    60301 tcccaattaa tatatttaaa gcttctccac taattccttc tgatagtttc ttttgcattt
    60361 ggtctccagc caaatgaatc aataaatcag ttacacaaaa tactgggtca ctatcttctt
    60421 ccccaataga tatgtccact tttgttccat catttaatat aactatacca tgtaaagcaa
    60481 gtggaatagc cgtccattgg tattttttta ttccaccata ataatgagtc ttaaaaaaac
    60541 ctagattact ctcttgatac agtggatttg gttttaaatc taatcttggc gaatctatat
    60601 ggcttcctat aatcttcata cctttttcta tataattttt acctattaca aaaagtgcta
    60661 ctgccttctc tttatttata acataaattt tatcaccaca acttatgctt cctttttcca
    60721 tagcttcatc tattgaaata tagttatttt ctttagccaa ttttataatt tcttttactg
    60781 aaagtctttc agtctttgac ttacttaaaa actccatata attgcgagaa tattgcataa
    60841 tttgttcaat agtattttct tttctttcaa aatcccaagc attcttaaat tcgtacttta
    60901 agttcatatt ttcctcctac taagttctaa tttttattgt ttttcatgta ttgctcatat
    60961 tcttctggtg aaactacttt gacactatct agtcttgatc taaagtgggc tctaaagttt
    61021 tctaaatatt ctttccttaa aatttctctt tctttgattt catcagcaga aaggattccc
    61081 tctttatttt tctttgctaa ttcattaatc ctatctaatt tcttcttgtc aaacattttt
    61141 tcctcctaaa aaacctctat ttatttaata aaataattta ttgcttttta tatctaactt
    61201 gtttttatat tcacaaaaaa actcatctat attatcactt attatagata ttcccttttc
    61261 tagttcttcc atgcttggac gagctatatt tatcctaaag aatctatcat ccactagatt
    61321 gtcaaaaaaa taagtccctg gaagcattga cactcctttg tttaacataa aatttgtaaa
    61381 tgcttgagat gtatatcctc ttggtaattc taaaaaaaag tttattcctc cacaagattt
    61441 tctaactttt aatttaccac tcaatttgtc attaatcaag ttttttgcca atttatactt
    61501 atcagtatat atcttctcta tatgactcaa ataattcttc caatcaaaat tattcatata
    61561 gtaatacatt gatttttgaa ttaaacctgg tgttgatata tctgatgaat atttagccca
    61621 taaaactttt tttaacaact catttggcat ttctacaatt cctatcctaa gacctggcat
    61681 aagtattttt gaaaagcttt ttatatatac tactctattt ttatcatcat aacttctaat
    61741 tgttctattg tcctttgatt caaaaacaaa atcacttata aaatcatctt ctattatata
    61801 aaaatcatgt tcttctgcca attctatcaa tttctttttt ttatatgttg agtaagatat
    61861 tccagttgga ttttgaaaat taggcataat gtatataagt cttggtttta ttttttccaa
    61921 ctttaatttt aaaataccta tatcaattcc atcatctaac ataggtatag atattatttt
    61981 tgcacctcta cttttaaaca cttcaattgc tccattgtaa gaaggttctt ccataaaaac
    62041 aacatctgaa taacttataa gtcctttaca aactatatct ataccttgtt gagcacctga
    62101 tattatttga atattttcct tagtagtttt tatatttcca tcacttagat actctaccat
    62161 tttttctctt aattcttcag agccaagacc ttcatcatat tcaaatattg aactaccatc
    62221 tttagataat gccatattta cagcattttt aaatgaatcg ataggaaaca tatcattaga
    62281 tggattccca ttgtcaaaat gaattaaatc ttctctttgc acaataatct tatttttcct
    62341 gttatctata ccgcttttat aagaatttaa acttgataca aaagtaccac tccctataat
    62401 tttatataca tatccttcat tttctaaaag ctcgtatacc ttaactatag tagtgttatt
    62461 tacttgtaaa aaatttgata gttttcttat tggaggcaat ttttcatgtt ctactaactc
    62521 tttatttatt atcaattctt ttatatgatt aaatatctgt atatatttag ttgatttttt
    62581 ctcatttaaa tctattctgt atttttccaa atttatcacc gctcattgta tttatatttt
    62641 attgatgctt ttatgtatat ttgatattat ttagtatgta tatataatac caaaattttt
    62701 ataaaaaggg gaaatattta tgttttatga ctatcacatg cactcaagtt tctctacaga
    62761 cggaaaatct acaatggaac aaatggtaaa aaaatctatt gaactcggtt tggaggaaat
    62821 ttgtttcaca gaccatgttg actatgatgt atatgctgat gattcttttt caattgtcta
    62881 tgaagattat tttaagagtt tagagactct tcaaagcaaa tataaagata aaatatcaat
    62941 aaaaaaagga attgaatttg gagttcaaac tcaattaatg gatacataca aaaaggaagc
    63001 acatcaatat ccattggact ttataatatg ttctatccat gctatagata caatggactt
    63061 ataccttggt aattatttta aagataaaac tcaacatgaa gtttatgaaa attactatct
    63121 ttatttatat aatattgtta agaattataa agattactct gtcttaggtc atttagattt
    63181 aattaaaagg tatgcacctt atgacactgt tttagatgat agactatttt ctgatataat
    63241 tgaagaaact cttaaacaag ctatttatga tggcaaaggt atcgagataa atacctcttg
    63301 ttatagatat aatcttccag acctaacccc atctaagtat atacttcaaa tgtataaaga
    63361 cttgggaggg gaaatcataa ccacaggttc agattcacat catataagtc aagttgcatg
    63421 tgagtttgac tacatatatt cattactaaa aaatatggga tttaaatatg tatctaaatt
    63481 taataaattg aaaccagaat ttataaaatt ataaattttt acacctcctt gtgaatatat
    63541 ttaaaagata aggaggtgat ttttttgaaa gtaccttatt tagtactatc aacagttatt
    63601 ttatttatat ccaattttat tgtaagaata tttggttttt tatataaaat attcttatca
    63661 agagcactag gtgaaacagg tttaggtatc tatcatatga tttttaactt tttgatgatt
    63721 tgcttagctg ttacaacaac aggtatacct actgcactta gttgtctagt tgcaaagaga
    63781 aaagctttaa acgatagaca caatactaat gctttattta tatcaacttt atatatatca
    63841 tttttcgtag cactaattat atctatagtt gcatctttca atagttcttt tttatcactt
    63901 aaatttttaa aagatgctaa attaaatcta tttatactag ctgtttgtcc tgctatagtt
    63961 ataataactc tttcaaacgt actaagagga tattactatg gcataaagaa tgtaaaaatc
    64021 cctgctattg gacaaatcat tgaacaaatt ggtaaaatat tatttgtttt tttacttgtt
    64081 atgtacataa ataataaatc tatgaattgt tatatagctc tattaggtat atcgattggt
    64141 gagttgagta atattatatt tatgcttatt tgcttatggc gagattcatc atttgacaat
    64201 aggtatatta taagcataaa agatttttat aattcttcta tggaaacatt aaaaatgtct
    64261 attcccataa cttgtaatag aatgtcaaac atacttttgc aatctataag ttctatgatg
    64321 ataccatcaa gattagcatt atcaggaatg acttatcaac aatctttaag tatgtatggt
    64381 attgtcagtg ggatggtaat gccttttata tttttacctt ttactgttgg ttcagcgctt
    64441 attgtaaatt taataccaac tatttcacag gaaatggctc taagaaagcg taagagtgta
    64501 attaaaaaaa tcaaatactc catacttctt actttatttg taggcatact atcatcaata
    64561 tttttttatt tctttggaaa agatttatgt atattagtat ttaaaaatac acttgcagga
    64621 gaatatctaa aagctatgtt tttagttcca ctatttatgt ccctaaatca aactctatct
    64681 ggcattttac attcaataag aaaagaatta gcttcaagta taaatactat tactggcatg
    64741 ttaattcaac taatagcttt atatgtattt cttccaattc caggacttaa tatttatgca
    64801 tacatataca caatgacgat agtatcaatc tttacttgtc tattacatac tatagtgctt
    64861 tttaaatcac taaaatctat acgctagtta taaaaacgtg aaggctacca atgtaaatta
    64921 tacactttgg tagccttcct tagatgattt ttatttataa tttaaactgt ctatatatct
    64981 acctatctat ctgtggcaac ttggctttcc acttccatga ttaggtccac attgcgaaaa
    65041 atcacatact ttagagaaag tatcaacaag tcttggttgg attggatttg gtgatgctaa
    65101 aactactatt tgttcactta ttaatgtgct aactgtttct tgtaagcaaa gtactaaatc
    65161 tatatcattt ggtataatta attcatagaa aggttcacaa cattcgttgc attctaattt
    65221 taatatagat tttcctatac aatctacagt taagttagtt tgatattctt gtcttaaagt
    65281 aacagctctt ccgtcagctg gcaagcatat tggtgctgaa agtgtgttaa aagtaaattc
    65341 tacataatca actcttctct tacatccacc tcctgctcta actgctggga aagctaatgc
    65401 aacgaactct gtacatccac atctacctct tagctctagt actaaattac gaactgctac
    65461 agttaatcct ctttgtttat aagctactga tttgcctttt ccttgtctac agcactcaga
    65521 gtttctttct ccacaaactg caaattcaaa agttgtttca cagtttcttc ctactatatc
    65581 tcctaatggg tctaaatcga aatcttctaa agttgtcatt cctgtgtcta taacgattcc
    65641 atcattactt aagcatattt tttctatagt tacacttgct tgacctgcac ttggaactga
    65701 accaaatact tctactactt ctgtttcaaa agttaatggc tctccatttg gtcctgttgc
    65761 atctgtaaat gtttggaatt gcatagtatc aaatattcta ttagcctcta ttgcaaatgg
    65821 cactgcattt gatacagaga agtttcttgg agatatagga ttacagcatt ctggtccaca
    65881 tttgttttca aaacattcat ctgaatccat ttcacatggt tcacaatcac atttacaatt
    65941 gtcatggcat ccacaattgt catcacatgg attaggtttg catggattgc atgggttagg
    66001 tttacatgga ttacatgggt tgcatggatt gcaatcatca tggcactcac acttattatc
    66061 agtattacat ccattatgat ggtgtgattt tttaaaacct ctcatgtctt ctgaatttat
    66121 ttcatctgta tacacttctt cttcattcat tgtagacatt ggctgattca tcattcttct
    66181 tttatttttt ttataatctt gcatttttca ttgccctccc tgtaagcgat tcttattaag
    66241 tctttggctt tttgaagcct aattaatact atgcttttat aatcttaatt gttacttgct
    66301 ttttgaattt atcaataatt aggcgtattt agtagaaaaa tgcatattaa taaaaaaagt
    66361 actactattt gaaatagtag cacttttttt gtcccgaaac accggtggct aagtaaattc
    66421 acaccctagg cactcccagg tgggtgactt gccagtaacc tttaacgtcc aaactaacta
    66481 ggcatgttat aatttaccag acacggtctc ccgtatttca tttgtaggtt gaatttaaaa
    66541 aagccttatc gtatgtttca ggatttcata ttattattat ataataaatt ttgaattttt
    66601 taaagtggtt ttatatagta attgagataa tcttattcta tcattacttt ttcttccttt
    66661 tttctttatg tatttagtta attatcgttt tttcttagct ctagttttag tatttttttc
    66721 attataattg tattcggatt cttgctcatt aatgttgtat agttcctcat cgtacactat
    66781 atcttcatca ttttctatat tttcatagcc tgtgttctta tcttctacta attcatcttc
    66841 attatcctcc atttcacctt catctgttaa ctcatcatca tccaattctt tgtcttcaca
    66901 atctaattct tcgtcttcat aatttaactc atcattattg tcttcatcat cattattact
    66961 atcatcacct cttaaaagac ttcctaacaa tttgagatta ttcatactac caagattgtt
    67021 tatatttgcc aaagagctca aactaaatcc accttcacca aagtcatctg gtaaaaataa
    67081 ttctgcaaat aaatctatct tctttttcat tcctatagct ttttcaactg aattttttct
    67141 tttttctggg aatacatcta taatttcctt tatcatcata ttctttttat catcactatc
    67201 catttttctg aaaaaatccg tttcatcttc tgtatctacc atatctatat tatttaattt
    67261 ttttattcct ctaactaaat ctaaaacact ttctattttt attagtattt ttctttcatc
    67321 tcttgacatg tattttttag ttcttgttat tatttccatt cccatttcta aatcttcttc
    67381 acttaaatcc atgagcctta aacctctttt tatatcattt gcatcaaacc ttaaattgcc
    67441 atgtgtagac tgtgaaagtt tatcatttgc attttttttg ttttttctaa ctctattatt
    67501 tccttttgat ttatttttat catcaaatat agagatatta ccattactca atgcaatcac
    67561 tccaatcaaa aatagtaagt tattcaagac aattccccct tatattatat aattgcccta
    67621 tattaaagta tgtttttcat attttaatgt tccattttta aagctcagtg catatatatt
    67681 atctataaga atttttattt atattatata tcactcacaa ttcaccactt caatattatt
    67741 tctatatata aatagtatga atataattca atatatattt atagaaaggg gtttttttat
    67801 gaaaaaatta gatacagagt cattagaaaa aatggctaaa caaaaaaata ttgacaagga
    67861 taaaatagaa aagatggctg atagttacaa aggaaaatca gaaaatgaac ttatggagga
    67921 acttataaaa ataggtaaga atctagatgg cagagatgaa gtagtatcaa aattcaaagc
    67981 atttttagat gaaaatcaac gcaaaaaact ggataatata atggaaaaaa tctcagatgc
    68041 agaaaaccaa agagatacta aaccagctaa aactaagaag gctaaaccta ctaaaggtaa
    68101 gtcaaattca cctgcaccaa aaaatacacc aggtagtcaa caaaaaaaat ctaaaagttt
    68161 atttaagaaa actaaaaagt ctaaccctca tgaatagata aaaataaact atttaagtaa
    68221 ttagataatt atttttagtt aatctattat taaatttgat attattgaca taaaaaatca
    68281 ggtagtgcct taggcactac ctgattgatt ttaatagttt tatttttttc tttctgtaac
    68341 actttctcta tcaacaattc tataaggtaa ttctattttc ttattttcaa ttaattcctt
    68401 atttattaat tttataacca ttcttattgc tactgctccc atatcgtata gaggttgata
    68461 tactgtagta agtttaggtc tatacattct agctatctta acatcattaa atcctgcaac
    68521 agatatgtct tctggaacct tatatccagc atcacatata gcatttatag cacctatggc
    68581 agcctcatca cctgttacaa aagccgcatg aggtattatt ccatcatcta atagttcttt
    68641 catactattg tatccactct cataatcagt tccaccatac ttaatcaaac tcttgtctaa
    68701 ttctatattg ttatttgaaa gtgctttctc ataaccagca agtctttcca tttctaaaac
    68761 agtatcatct ttactcgtca ttataaaagc tatcttttca tgacctttct ttataagatg
    68821 ctctgtcata tcaaaagtag cttcttcgtt gctagtactt actgtatata tatcataatt
    68881 tctagcagtt ttacttatat atgtcgctgg tattccacaa ttttgtatat agttgatatg
    68941 ttcttgttca actatccatg aaatcataac tataccttcc acttgttttg ctctcaatag
    69001 atttatactc ttaagttctt gttccttatc agagtatgta ttcgctaaaa gaatatcata
    69061 gtcatacatt ttagcaacct cttctatccc atttaacacc tcattaacaa aagaatctga
    69121 aacttctgga actattaccc ctattaattg actcttcttt gttactaagc ttctagcaag
    69181 tggatttggt atatatccag tctcttttat aacctctaaa actttttgtt tgacttcatc
    69241 agttacaggt tttgaatcat ttataactct agatacagta gatattgaca ctcctgcttg
    69301 tttagcaaca tcttttatcg ttatattgcc tttcatcttc atcctcctta ttttactaaa
    69361 tttatctaaa actgaaccaa atttttgaag gttcctattt taactacatt tatataagtt
    69421 tttattttac cataaaagca agtacattta aatacattat attttatatt ataccctata
    69481 ttcaacaaat ttaataatat tcattcttaa tttaaatcat attaaaaaat aaaataaaaa
    69541 aatgctggta taaaatacca gcattttttt attctaatga tttaagtatt agcagccgca
    69601 acctacttca ccatcaaaga atccaccaca tgaacaagtt attagcaata atattaagaa
    69661 aggtatccat gacattatgt caatgtctct ccagcaatct tcaaaagcta agaataggaa
    69721 gaatagtact attatccacc atcctcctcc tccaaagcat ccttcttctc caaaaaatct
    69781 atctaacata acaaagccct ccttttttta ataactgtgt tcttattcta agcatccacc
    69841 acagcagcaa agtattaata aaactattag gaatggtatc catgccatta tgtcaaattc
    69901 tgcccagcta tctccaaaag ctaagaatag gaagaatagt actattatcc accatcctcc
    69961 tccaccaaaa catccttctt ctccaaagaa tctatctaac attatacgtt acctcctgtt
    70021 gatattttgt tgagcttttc gctcctaaca tatactatgc atttggtttc cattttgcta
    70081 atgtattttt actatttttt taaatttttt aatttgaact aaaaaaggta aaaccataac
    70141 ttgtataagt tactggtttt acctatttaa taatcaatat tttttagcaa caacaaccat
    70201 catcgaagtt aaataataac aatactatta atataaccca tatccatata ttgtcatcgc
    70261 ataataagtc ttctaaaatg tctgtgcata atagtaagaa tactataact attattatca
    70321 acattttatt cccaaaaaat ccttcgcatc ctccaaagaa acgttccata ttgtcttcta
    70381 attttgtcat aactaatcct ccttgaatca ttaagagact aagttttgtc tctttaatta
    70441 aatatatgta gtaatagaca gataagttac ttctaaaaag tttttattga ttagtttatt
    70501 tatttctttt tcaccaagtt tattgtatct atcaacttca tccagataat cttttttctc
    70561 ctctgatata gtatagatta aactttctat tgattttatt ttgtactcct tatatctttc
    70621 tatcaagaag gatgtttttg aattttctat tcctttcttt acatgcatta accttgatat
    70681 gtagtcttct ttttcattag atacataact ctccttttca gctacatctt caaggattaa
    70741 attggtctcc attacataat cctctatacg ttgcatatct aaagttattg aattataata
    70801 aaaatttact gctataagta aaaacattcc tactgaagtt agtaatagtc ctacatattt
    70861 catagcattt cctcctaagt tatatttgtt atcaataaag tttattcatc aaataatact
    70921 ttattactac atttataaaa ttttattatt ttttatagat tccatattga aaaattcttg
    70981 atattatatt aaactaacat taaaattaat taaaactgct ttaggaggct taaaatgttt
    71041 gagaaaataa aagaaataat agcagaacaa ttaggggtag acaatttaga tgagataact
    71101 atggaagcat ctttaatgga cgacttagaa gctgattcat tagatgctgt tgaaattata
    71161 atggcccttg aagatgaatt tggaatagaa ataccagacg aggaagctga gaactttaaa
    71221 tgtattggtg atatatgtaa atatatcgaa gaaaacaaat agtatcgttt tgtctacaaa
    71281 tataaatagt ctgtgtaaac ttctacataa taaattatat tagagttcgc acagactaga
    71341 ttacatttat ttacaatata taaaatacac gctcttatat ttaatttata tactaagtat
    71401 ataaattact tttcttcata aaacgtaaca ttctttatta ttatatctat agttccatct
    71461 tgattttgtt ttacagtata cttcattggg tcttcaaaat cagttagatt cccttttatg
    71521 tcaaaaccat tatccgtttt tatacttctt tttttaagct ttttctcaac ccatttttta
    71581 tctatactaa aactttcatc aagacctttt tcttccatat gctctttaaa gctatccttt
    71641 aactcgtcat ctttaattga attatcaaca aagtcattta tatcaacttc atgtttttcc
    71701 ttcagtgtat aatttaatat acttcttaca tcttcagctt gctttatatc attactaaga
    71761 gcattggtta tccaattttc agctgtattt ttaaatacct tggtcttgta cttatcatca
    71821 tctatttttt tagcatttaa aaattctgtt acaaacttag attctgaccc ttctttttca
    71881 gcatccttat ctaaaagcct tagatgaaac tcatcattta taccacttag actaatcaaa
    71941 gctccttgct tttgtcttaa agtctctggg ataccaattt catttgaaac catttgaata
    72001 ttaaatttat catccacaaa ttcaattgaa tgagtgtaca actttttata atctaatttc
    72061 agtattgcta cactcttttc atctttaata gtgtataaac aaattgccaa atcacaagat
    72121 tctagtgcag cattaatttt cataacgtca aacaagtaag atgcaatttc tttagaattt
    72181 ttcaaaaaag tactttcatc atatattatt tgttcacaac agtttttaat taaattgtca
    72241 ttataatctt taaaaacacc ttttcttaaa tcctcatctt ttgcaattct ttttatagct
    72301 ttctggaaaa aaccatctac ttcttgatta actttgcctt caaagtcatt taatattgga
    72361 acatcactat ttttgtccaa aacatgtatt ataaatttat gtattatcat atatatcacc
    72421 ccattatctt aatttcgata ttaactcttt catatcctct ggtatatctg ctttaaattt
    72481 caatttttct tttgttcttg gttgttcaaa ctctaactta tatgcatgaa gtgcttgcct
    72541 atttattagc tcttcatcta cataaccata caactcatca cctattattc catgacctat
    72601 atgagccata tgtactctta tttgatgtgt tctaccagtt tctaagctta cttctaatac
    72661 agttgcattt tctagtcttt ctattacttt atagtgagta acagatgact gtcctctttc
    72721 atctattatc ctctttattg aatcttctgt aggtctataa attggctcat ctatagttcc
    72781 ctcattttct tttataattc catcaactac tgttatatac tttttttcaa ctttattttc
    72841 actcattgct gtagacaaag tatgatgtgc ataagcattt tttgctacta taactaaccc
    72901 tgaagtattc atatctaacc tatttacaaa tcttattttt acattttctt tttgattatc
    72961 tatatagtaa ctaataccat ttgctattgt tttatcatag tggctttttg ttgggtgaac
    73021 taccataaac ggaggtttat tcaccataat tatatcaaaa tcatcataaa ttatctgtaa
    73081 atttaaatct tgaggttcaa aatttgcttt ttcctcgtct atttttactt ctattaaatc
    73141 accactatac acctttaaac tcggtttttt atatactcca tttactaaaa cactcttttc
    73201 cctcttcatt tttgataatg acctaactga gaaatttagc ttgtccaata aaacttcttt
    73261 taaagtcatt tcttcttcat ttgtatacga tataagatta tatctttgat tctctttttt
    73321 aaacaaactt ttacctactt tcttttatat cttcctactt tttatatctt caaaatcatg
    73381 atattatata ttttacacta tttttgttta atagataata attattttta actataaaaa
    73441 cctgtaagaa taacaaatag ttattcttac aggttaaatt cataattaaa tatttaattt
    73501 gtaaatatta taatctaaaa ctatctctta actattaaag tagttcccat tcctccacct
    73561 atacaaagtg tagcaagacc agttttagca tctcttctct tcatttcata taaaagtgta
    73621 gtaagtattc ttgctcctga gcatcctatt ggatgtccta tagctattgc tccaccatta
    73681 acattaactt tgttcatatc tatatttaag tctcttatta cagctacaga ttgggcagca
    73741 aatgcctcat tagcttcaac taaatctata tcttcaatag tcatattagc agcttctaaa
    73801 gcttttttag ttgctggaac tggtccatat cccattattt tagggtcaac accagctgtt
    73861 ccataagaaa ctatagttgc aagaggctct attcctagtt cttcagcttt ttctttagcc
    73921 attactacta gcatagcagc accatcattt attcctgatg cattaccagc agtaactgtt
    73981 ccatcttttt taaatgcagg tcttaactta gcaagtttct ccattgtagt gccaggctta
    74041 atatattcat ctttatctac tacagtgtca ccttttcttc cttttataac aacaggaact
    74101 atttcttcat caaattttcc ttcagcttga gctttttcag ctttattttg acttgcaaga
    74161 gctaattcat cttgttcttc tctagttata ttccattgct ctgctatgtt ttcagcagta
    74221 atacccatgt gatagttatt aaatatgtct gataatccat cttttatcat tgaatcaaca
    74281 aaagcagcat cacccattct tgcaccatat ctcgcacttg gtactaaata aggagacata
    74341 ctcatgtttt cagctccacc aactaacatt atatcagcat cacctaatgc tataagttga
    74401 gatgccattg aaacagatct taatccagaa ccacaaacta tatttatagt catagctggt
    74461 ttttctactg gtattcctgc tcctaatgct atttgtcttg ctatattttg tccaagacct
    74521 gctgtaagta ctccccctaa aagagattca tctatcatat ctggagttat gttagctctt
    74581 tttatagctt ctttagctgc tgttacccct aactctaccg ctgaaactga tttaaatgct
    74641 cctccaaaac ttcctactgc tgttctagct gcactggcaa ttactacttc tctcatattc
    74701 attaccccca ttaaattaga tttttcatat atccgaaatt gttttttaaa caattttatt
    74761 taaattttta attcccaaat tcttatttat tataatcata gaatcctatc ttagtttttc
    74821 ttcctaattg attagctcta accattttag ctaaaagtgg atgaggtcta tatttagtat
    74881 ctccaaactc agtatataaa acgttcatta tggctaaaac aacatctaat ccgattaaat
    74941 cacctaatgc tagtggtccc attggatggt ttgctcctaa tttcatagct tcatctattt
    75001 cttctttact tgcaacacca tctgcatata taccaacagc ttcatttatc ataggtataa
    75061 gtattctatt tactacaaat ccaggagatt cagatacatc tactggtact ttattgatac
    75121 tcttagataa ttcaaatact gtatcaaaag taacttttga tgttaactga ccacttataa
    75181 cttcaactaa tttcatcata ggaactggat taaagaaatg cattcctata actttatctg
    75241 gtctcttagt agaagaagct atttctgtta tagataatga tgaagtattt gttgccaaga
    75301 tagtatcttc tttacataat tcatctagta acttgaaaac atctttcttt atattcatgt
    75361 cttctacaga tgcttctatt attaaatcca tatcttttaa atcttcatag ttagtagttg
    75421 aactaacatg acttaatatt tctgcttttg tagcttcatc catttttccc ttagtaacta
    75481 acttagttaa atttttatct aataaagcta aacatttatc tatagcacct tgagttctac
    75541 tctttaaaca tacatcatgt ccacaacttg caaaagtttg tacaatacca cttcccatag
    75601 ttccactacc tattacagct aatttcatag ttataacctc ctgttatttt agtatttata
    75661 ttctgttaac tagattaaaa tactatttaa tctagttaac agcaaatata tttttaatta
    75721 gatttttacc cttttataaa gttagcttct ctcttttcaa cgaaagctga cattccttct
    75781 ttttggtctt tagttgaaaa acaaagacca aataaattag attctatatc tattccagta
    75841 tttatatcag tttgagcacc aagttgtatt gcttctttag agtatctaac tgcaagctga
    75901 gcatttttag ctattatctt agctaatttc tcaacttctt ctattaaaat gtctggctca
    75961 acgactctat ttactagccc tattttttca gcttcatcag cttttataac ttgacctgta
    76021 aagattaatt cttttgcttt tgccattcca accaatcttg taagtctttg agttcctcca
    76081 tatcctggag ttattccaag agttacttct ggctgaccaa atttagcttt agcagatgca
    76141 attcttatat cacatgccat tgcaagttca catcctccac ctaaagcaaa tccgtttaca
    76201 gcagctatca ctactttttt actattttct atttctccaa aagcttttgc tcctaagata
    76261 ctaaaatctt tagcagctac agcatctaaa tctttcatgt atgcaatatc tgctccagct
    76321 acaaatgcct ttccttcccc tgtcaatatt acaacatcaa tagtttcatc attattaata
    76381 tctacaaata cttcataaag ttcttctaaa gtctttgaat ttattgcatt aagggcttta
    76441 ggtctattca ttttcactgt acatatattt ccatctactt caacagctac attctcataa
    76501 actttaacat cacttgtact cataataccc tccccaagaa attagttagc taaaacttca
    76561 cctttttctt ttgcaacact taactgactg ataagttctg gaagcacttt atgaacatct
    76621 ccaactatac caacatcagc atatttaaat attggagctt ctggattttt atttatagca
    76681 actataaact cagcatcttc cataccagct atatgttgta ttgctccaga tataccacat
    76741 gctatataaa ggtctggtct tacagtttta ccagtttgac caacttgtct tgctttatct
    76801 aaccaacctg catctatagt ggcacgagaa ccagaaactt ctccacctat aatttcagct
    76861 aattcataaa gtatgtctaa gttttctttt ccacccattc cacgtccagc agaaactaat
    76921 atcttagcat cttctatttt aacttgtttt ttagcttctt ttattacttg tacaacttga
    76981 actaatttat cagcatcatt aaattctacc ttgaaacggt taattacagc ttctttagtt
    77041 tcatcaggtt catttttctt cataacccct ggtctaactg tagacatttg aggtctgaaa
    77101 tctttacaaa ctattgttgc cattatattt ccaccaaagg caggtcttgt cattaataat
    77161 aattttgtat cttcagctac tgcaagacct gtacagtcag cagtaagacc tgtatgtatt
    77221 ctagcagaaa ctctaggtgc taaatctcta cctattgaag ttgcaccaaa taatacaact
    77281 atagggtcag ctgcttttat tgcttcataa gctgcttttg tatatggttc agttgtatac
    77341 actgctaaag cttcatcatc tactactatt acctcatctg caccatagtg tgctaatgta
    77401 tctattaaac cttctacctt actacctaaa agtaatgcag aaacttttgt atcataatct
    77461 tttgctattt ctgtagcctt tcctagtaat tctaaagaaa cagtttgaat tacattttct
    77521 ctttgttcta ttactactaa aacgttaccc attctttatc cccccttaaa ttatatgata
    77581 tacttctctt ttaatttatc tatgataatt ccagctgatg ttttcgcatc ttcattgtat
    77641 attgtaccag ctggtttaac tgattttgta aatgatttaa atacactagt tggagaacct
    77701 ttaagaccta aattagaagg gtcaacttct atatctttta cagtccatgt ttctactaca
    77761 tcattttcga aagcatcata tattcttcca actttcatgt atcttggtgt gttcatatct
    77821 ttaagagttg ttataaggca tggcatttta acttttaagt catggcaaca atcttcaaat
    77881 tgtcttttta ctagtacata ttcaccttca gtttttattt cttcagcata tgttattgat
    77941 ggaagattta aatgttcagc tatttgaggt ccaacttgtg cagtatctcc atctatcgcc
    78001 tgtcttccag ctattataat atcaaaatct atatttttta atgctcctgc taatgctgat
    78061 gaagttgccc aagtatcagc acctgcaaat gctctatctg ttaataatat acctctatct
    78121 gcacccattg ctaaagcttc ttttaaagcc atatctgctt gaggaggtcc cattgttata
    78181 acagttacat gagcacccat ttcttctttt aattttatag cttcttctaa acctgcttta
    78241 tcatcagggt ttattatact tggtactcca tctctaatta aagtacctgt attaggatct
    78301 agtttaactt ctgttgtatc tggaacttgt tttatacaaa cgactatatt cataaatcct
    78361 tcctccatat ctattttaat atcgacctat tgtgtatatt ttaatattta tctattttaa
    78421 taattttcct gaaataacca ttctttgaac ttcactagtt ccttcatata tttcagttat
    78481 cttagcatct ctcatcattc tttctactgg atagtcacga gtgtatccat atcctccatg
    78541 aagttgtaca gcttttgtag taacttccat agctgtttca gctgcaaata attttgccat
    78601 tgctgcttct actccataag gttttcctaa gtctttattt atagctgctt gatatacaag
    78661 gtgtctagcc gcttgtacct taacttccat atcagctaat tggaattgtg tattttggaa
    78721 ttttgataat ggtctaccaa attgtactct ttcttttaca tatttaacag tttcatcaag
    78781 agcaccttgt gctaaaccta aagcttgtgc agctatacca attctaccac catcaagagt
    78841 agacattgct atcttaaatc cttgaccttc ttttccaagt aaattttctt taggtattct
    78901 gcaatcctca aatattaatt cactcgtagc tgaacctctt atacccattt tcttttcttt
    78961 aactccaaag ctaaacccag gagttccttt ttcaactata aatgctgata ttcctttgtt
    79021 ccccttagat ttatcagtca ttgccattac tacatatatg tcaccagcta ttgcatttgt
    79081 tataaatatt tttgagccat taagtatgta ttcatccccg tctaaaacag cagttgtttg
    79141 ttggccagac gcatctgtac cagcattagg ctcagtaaga ccaaatgctc ctaatttttc
    79201 tccacttgct agtggtctta agaatttttg tttttgttct tcattaccat attgatatat
    79261 aggccatgag ccaagagatg tatgagctga taatataact cctgtagtac cacaaactct
    79321 agacaattct tcaactgcca ttatatatcc tacagtgtct ccaccttctc caccatattc
    79381 ttttggatat ggtataccca tcattcctgc ttttgccatt ttttccactg tttcataagg
    79441 aaatctttct tcttcatcaa gttctgttgc taaaggttta acttcatttt cagcgaagct
    79501 tacatatagc tctttaagca tctgatattt tttagaattt aaatccataa tagcctcctc
    79561 agtattttat aaatatttta ttttcattta ttaattaaaa tctcggcaac atctataata
    79621 aggtatttga atatacgtcg agaaatttat aaccttaatc aaactacgct ctatatttat
    79681 atgtgattat actaaccaat tatgacatta atttaatcat aaattgttaa tttaaaatca
    79741 actatactta aaaaaaatct cgctttttgg aattttgtga aatttcaagt tagaataaga
    79801 tttctttcac actggttaca gataaaataa tttgtttacg atatttatat tagggtatat
    79861 taaacattaa atattgtctg tacatctgtt ttttagccat gtacagaaga tttttatcgt
    79921 ataaaaatta ttttaataga gctaaataat cgtattttta tctgactatt ttttattttt
    79981 aaatatctta catctataat agtatcactc aaaggataat ttttcaataa tttctaatta
    80041 tttttttaac aatatttatt acattttttc cacttttttg tcattttttt attgtttcga
    80101 aaaaaaatca gaatttttta actttatttt ggattattag tttaaattag attttcctct
    80161 tagatttata aatatttaca caaaaaaact acctctgtat agattgtata cagaggtagt
    80221 tttttactaa ttcctactat agtttactat atttttttac caattcttac aaacatctac
    80281 ccatttttca cacttagaaa acttctccat ttcttcacat gtaagttcta ttgcagagtt
    80341 actactgcca catgcaggaa atactgtatc aaatcttttc attgaatcat ctaaatagac
    80401 ttttatagaa ttttttaaac caaatggaca gactccacca atagcatgtc cagtaaactc
    80461 caagacttct tctggtgtca acattttagc cttacaatta aattcagcct tatacttctt
    80521 attatcaatc ttagcatcac cagctgtcac aattaaaatt gcatcatctc ctattttaaa
    80581 tgagagtgtc ttagctattc ttgctggaat aaccccagct gcttcagctg caagttccac
    80641 agtagcacta gattgttcaa attctaatat actgtcttcc ttgccaaatt gtttaaaata
    80701 ctctcttaca ttacttattg acattaaata cccctcctag tctgaacttt tataaatttc
    80761 atattattct taattatatc tataattgat ttatttttca aattattcca taatattagc
    80821 attccatgta tcattatttt ttccaactac tagataaagt ccttttgcca ttggaatatt
    80881 gacaatatat tctcctttat actctacact cataccagat tcattaaagt acctataatc
    80941 aaaatttagt ccttttggaa ttacaaaatc aacataacct tgattatttg gttttattcc
    81001 atatttcaga gttctttcag ttatagaatt tttttcatta aataaatcta catttacata
    81061 tgcagaacac ttattcattc cctttttatt ttccacacaa ttttcaaact tcattttagc
    81121 actgtttaat ttataactat atttactatt attgcttatt ttaaatctta caatagtacc
    81181 aacatctcta cttctcctgc tatcaaaatc aaaaacaatt tcctctaaat gatttatttc
    81241 ataatctttg acacttacca tatctctata tactaattta tttatagcta tgaatagtgt
    81301 aaaaatcccc aaaataaaaa atgaaacttt tataaacttc ttcattttta gtctccttat
    81361 attttgtgct attgtgctat aatattaatc aaaattttaa taattaaact tattcttttt
    81421 tgaaatctta atagaaaaat acattgatat tataaaaatt attattgtta aaaatgtcat
    81481 actatataaa tttatatact tagtattagc tatatcagtt acataatttg aaagatatac
    81541 tcctccaact ttactataat taaaatgctt tacttgatat acaatcatac taatcatctt
    81601 caaaagactt ccatatacaa agtaaattaa aatacttact ataaaaccac aaactttcat
    81661 ttttccatat ccaaacttaa aaattaaagg cagtaaaatc gtcaaaattg ctaaaaatat
    81721 atttacagaa aaaaatacat catttaaaac catatttcta aagaacacta gtgatgtcaa
    81781 tttacttagt aaggacacaa ttattgttac agaaattatt aatcctacac ctaataaata
    81841 ttttgagtaa actatatctt ctatactaac tggcatactc cttataaaaa tccttgaatc
    81901 attttcataa tcacactcaa acgtatttat aaatatcaaa taactaacaa caatacttac
    81961 tgtataatat gaaattgaat taaggaaaaa ataaaacaca aaaaatagca atatgtactt
    82021 taaattacgg ataccatctg aactaatccc tattattaaa tcttttctta ttaaattgaa
    82081 catcatctca cctcatttac ataataatac attagttctt ctatagtagg atttttgacc
    82141 ttatgcttat caatttctat caataggctt tttactttca agctatctcc aactttaaca
    82201 agtgcctcat tataatattt atactctttc acaccaacta tgctttcttt aattgcttct
    82261 aactctgctt tatttgccct aattatctta tattcttgtt ctattaattc tttcttaaca
    82321 gtaaaaatct gatttcctct atttataaaa acaagataat cagcaatttt aattaaatta
    82381 tcagtattat gagtagaaat tattacacta ctacttccat ttttcacaaa atcttgtaaa
    82441 tactgcatta tttcagttct tacaattggg tctataccag cagttggttc atccaaaatc
    82501 aataacttag cattgtgact aagtgcgctt gataacataa gcttttcaga ttgaccttta
    82561 gataaatttt caatatgcat attttcttct atatcaaact tattaaggta ttcatcaaac
    82621 ctttctctat caaaattctt ataaaataaa gaaactattt ttctaaattc cttaaccttt
    82681 aaatgcggat aaaattgtaa agaatcatat acaaaaccaa tattgttttt aaagcttatt
    82741 ggagaatttt caatagtttc attacaaagt tcaatttcac cttcatctgc atcaataatc
    82801 cccattataa tttttattaa agtcgttttt cccgaaccat ttggtcctat aatcccaaca
    82861 atatcccctt cctttaactc taagtttata ttatttaact tgaattttcc taattttttt
    82921 gtgatattcc ttatgctaaa cacatcttca cctcttatgc atttttttat acccacctat
    82981 acatatttta acacattcat tacaaaggtc atttataaat ttataaatta agaattataa
    83041 aactatgtta taatacaaag tatgaagagg tgaatatatg aaaagaatta taactataaa
    83101 tacaaaccaa ttaaataagt cccttgagac taaagaactc ttaactagaa aacttatcaa
    83161 tgctggcttt gaagtgtatt cagatattta tccagacaca gagcttatta tatcaattgg
    83221 tggtgatggc tcttttttaa gaacagttag agatttcgat tttcctgaga ttcccataat
    83281 gggaataaat acaggtcatc tggggttttt tcctgatatt ttaccagata agattgatag
    83341 ctttattgaa gcttatacaa aaaaagatta tattattcaa gaaatgtcac ttcttaatgc
    83401 ggaagtatat actactacta gtggttctaa tatgcttgct gtaaatgagg ttgttataag
    83461 aggagacaag tctaggacta tacacctaaa tttaagcctt gataataaac atatacaaaa
    83521 ttttagtgga gatggtatga ttatttctac ttccacaggt tcaacagcct ataactattc
    83581 tgctggaggt agtattgttg atataaatct tgaacttatg cagattacac ctttacatcc
    83641 gataaatacc aatgcatata ggtgctttac atcaagtata atatgttcaa atgaatctgt
    83701 tataaagatt gcaccagaat atagatttga agattctgta ttgattgtcg ttgatggagt
    83761 tgagcacaga tttagacaaa tcgaaaatat aaaagttagt atatctgatg caaaaataaa
    83821 attacttaga atgtcaaact atgagttttg gcatagagta tctgaaaaat ttttatagac
    83881 tgtacttagg aggattttta atatgaaaat aggaataatg agtgatactc atggtagctt
    83941 actttacttt gaaaaagcgt taaatgtatt atctgattgt gatgttttat tacatggtgg
    84001 agatgtctta tatcatggac ctagaaatga tattccagaa ggatataatc ctaaaaaatt
    84061 tattgagaca ttaaataaat tagaaaacat tgtgattgta aaagggaact gtgatgcaga
    84121 tgttgaccag atggttatag aacatcctat tcagagtcct tatgtcatga gccaatttgg
    84181 tgaaattaga attatattga atcatggtta tatagaatca gaagaagaaa ttattgacaa
    84241 agcaaagaaa atgggtggtg acatattagt tttaggacat actcatgtaa aaaaactata
    84301 catggatgat aatttaatag taataaatcc aggaagtact tctatcccaa aagatggaag
    84361 ccattctgtc gcaattatag atatcataaa aactgatgat gaggatgagc ttgaacttga
    84421 tataaactta attgacatca atactggaaa tataataaat atcaatgatt agtaaatttt
    84481 taataaaaat aaaataagct ttaatttatt aaactcataa taaaaacaag taaaccattt
    84541 tatttctatc tcaattagaa gtttaagtac aataccttaa acttctaatt aaatagaatc
    84601 tggtttactt gttttattat atgctatatt gaaatataac ttagtttact tgcttctatt
    84661 atatattata ttggagtata acttagttta cttatttcat tatatattat actgaaatat
    84721 aacttagttt acttatttta ttatatatat tgaagtgcaa cttggtttac ttgctattat
    84781 tatatattaa ttttgattta cacaatatta ttgtaaggct aatgcttctg catatggttt
    84841 actattttta cctccaccaa cttgtacaaa tttatcagcc tttacttttt ttataaaact
    84901 tttctgtgca ctacttaaac ttccacttgc aagtactaca ggtgactttg tttttgatgc
    84961 taatactcca gcagcaagcc catcaactaa atcagcttct ctaccacttc catcttttgc
    85021 aaaatacata ttattaacct tacttgattt tccataaaac ttttctaaaa ctttcgcatt
    85081 agtatctttt ctatctgtcc ctgctagtct ttctgagttt actaactgtc cttcaagttt
    85141 tttacttata ctgtactccc cacctactat gtatgtctta gttgagtttt gtgtaataaa
    85201 attctttgcc tcacttctta tgctatcctt acctgtatat attatagctg tattatcact
    85261 tgcagctggg cttgatattg aagttgcatc tgccagtcca ttatacccat taaccactgc
    85321 cacttttgag acacctaaat caccagtatc atctagctgt ctagtaagaa gtaaagatgt
    85381 ttcttctcta tctgctccac cgattctact gactaatact tccatatctt caagagattc
    85441 ttttacactt gatgaaatag ctccttcact tccaattaaa ataacttctg ctggattaag
    85501 cctttttatt tcctctgtta cattatatgg tatactattt ttatttgtaa gcagtattgg
    85561 cgcatccaat ttacttgcca aaggagttga agccaatgca tctggcaatg aatttgagtt
    85621 aactagaaac accctttctg aaccattatt ccaaccactt tgagatattt ttatcgctgt
    85681 atcaaatcta tcttcaccaa taagtttttc ctcctttgga gattttctac cttgaagata
    85741 attgttcata tctacctgtg cattttgatt tttactaata gtgtctgcat atacacaact
    85801 tggcaacata atcccaagtg aaagtgcaaa tgctattatt tttttagatt taattttcaa
    85861 tacaattccc ccttttaaat gttaatacct attgatattc atacttttta ttgataccct
    85921 aaactaaaaa taattatctt ttataaaaat aattatcttt tataaaaata attacaaaaa
    85981 tagctatacc attttaagta caacacaagc ttataatcag cttgacttat aaagaatgat
    86041 atagctatta taactttaaa tgcttaattt ttaaatactt tttaactaaa ctctttcagc
    86101 atatttttca gctattctta caattagttt agtagctttc tccatagaac taactggtat
    86161 acactcattt ttcccatgga agtttaatcc accagtaaat atatttggac aaggtaatcc
    86221 attaaaagat aatcttgctc catcagtacc acctcttaca ggaactaaac gtggttttat
    86281 acctaattcc tccatagctt ctttagctat atcaactata aacataactg gctcaatctt
    86341 ttctttcata ttgtaataag agtcttttaa atccaattcc actctattat cgtatttctt
    86401 atttattttt tctatattct ctatcataat agattttcta ttttcaaatt tttctttaca
    86461 atggtctcta ataatataag ccatacttac attctcgaca tttccaccta tagaatttaa
    86521 atgccaaaat ccttcatatc cttctgttgt ttctggtctt tcatctgctg ggaacatttc
    86581 tgatatttca gcagctatat gtatagcatt taccatttta tttttagctg aacctggatg
    86641 aacatttcta ccatgtatag taatggttgc atttgcagca ttaaaatttt cacattcaag
    86701 ttctccaact attcctccat ctaaagtata tgcatatttt gctccaaatt tttcaacatc
    86761 aaataaatca gcacctctac caatttcttc atctggtgta aatcctattt ttatatctcc
    86821 atgcttaatt tctggatgat ttattagata ctctattgca gtcactatct cagctatacc
    86881 agctttatca tcagacccaa gtagagttgt tccatcagtg actattaaat cgtcaccttt
    86941 aaattcttcc aactctggag aatcctttac atacgtaata acatttaacg cttcatttaa
    87001 aactatgtct tttccatcat aattttctat tattcttggt ttaatatctt ttccactaac
    87061 atcttcaatt gtatctaaat gtgatatgaa tcctatagta tccacaccat cagtatttcc
    87121 ttttaaagta gccattatat aactattttc atctaaactc acatcatcaa cacccattgt
    87181 ttttagctct tcaactatat acttagctaa aactctttga ccttctgaag atggacagtt
    87241 ctcacactga gaattagatg ttgtatcaaa tgatacatat tttaaaaatc tttccactac
    87301 tttttgtttc acgtatatac cctctttctt aataagatta atattttttt atttacctgt
    87361 gaataaaatt ttatttttaa aaaaatctat caattattat aattaaaaaa tatccacctt
    87421 cttaagtata tcatgcatac ataccaaacc caaacaaaaa attactccaa ttattccttt
    87481 tacttggatt ccaataaaaa tcgctgctag ggtagctaat ggatttagtc caacatttac
    87541 tgatactagt ttaggttcaa gtatttctct aatcaaagac aaaacaaaaa atatcacagt
    87601 catacttata gctataaaat aattttttat aaggaaataa taaacaatta ctggaagata
    87661 tattgttatt atacctagga atggaattaa atccattaaa gctcctacaa accctactat
    87721 tagaccgtat ttcaatccaa ataaagcaaa acttccccag attgcaaaaa atgttatagc
    87781 cataagtatt gtataggccc ttatatatcc aacaagtgaa agccctgcct ctttctttac
    87841 gttttttacc ttctttctaa catcaacagt aaacatatta taaaaactat tttcaatctt
    87901 atcaaggtct tttgctataa agtaggtagc aataaacagg gtaattataa atattataat
    87961 atatggaata gaagtagcca atccaagtat actagtaaga agattttttg taatttgcat
    88021 tacttcacca ctgaatttgt ttactaattg ttctacatca aaattggcta tttcttgaaa
    88081 tgatttcata aagtcattta ttttatttaa aaaatacata atagtattac ttatatcctc
    88141 aggattagca gatatattat ttaaaaatcc cattaattct ttcattgaat ttattactat
    88201 tattgtagta actgtactga ctatagccac tgcaagaaaa cttagaacaa gagtagagat
    88261 acctttattt atacgaaact tattttctaa cttttgtgat ataggattaa tcataagtgc
    88321 tattatagca cctagaaaaa atggagctat gtatggaaac gctttgtaaa taaaaataaa
    88381 taacaatgta tatatacaaa aaaagatagt gttgtttttt aatttgtata aaaaattatt
    88441 ttctagtatg ctcatcttaa aaatctcctt tcttctaatt tatattttta ttttgtccat
    88501 ttcatactag ttttatttga atacaaaata aaaagaagtg tataaatata aaagatatct
    88561 tttatattta tacacttcta ataaatttaa gaacattatt acattatgta tatattctta
    88621 tttttttaat tctatttttt tctatacttt ctatacaaaa tcttatatta tcaacttcaa
    88681 ttacttcatt ttcttctggc aatctgctta ggtgtccaat tataaaacct cctatggaat
    88741 caaactcctc agattccaag tttacaccaa taagttcatt aacatcacca attttagtac
    88801 ttccatctac aatatactca tcttctttta taacttgtat ttcatcctct tcttcatcat
    88861 attcatcctc tatatctcca acaatgactt ctactaaatc ttctatagtt aaaagtccag
    88921 atgttccacc atactcatct acaactatag ccatctgact tttttcaagt ttcatctctt
    88981 caagaagttg tgttatcttt ttgaactcat acgtgaaaaa aggttctctc atataatttt
    89041 taatatcaaa tgactctatt tcttcatcag ataaaaatat aatatctttt atattaagta
    89101 ttcctacgat atcatctata gtttcttcat acacaggcat tctacttaat ttttcagttt
    89161 taaatacctg tattatctca tcataactat cttccatatc tatagctacc atatctattc
    89221 tttgtaccat tgcattttta gcttgcatat ctccaaattc aaaaacatta tttatgattt
    89281 ctctctcttc catttcaagg acgccttctt catgacttac atttaccata gtttttagct
    89341 cttcttcagt tatgaatgat ttagctcctt tatttgttat accaaataat ttaaatataa
    89401 tatttgtaat tatattaaat atccatacta ttggtcttaa tataaatata attgctttta
    89461 taggtttaga tactaataga gaaacctttt ctgaattatt tgctgctatt gtttttggag
    89521 taatctcacc aaatattaat actaatactg tcataacagc tgtagcaagt gccactcctt
    89581 tttctcccat taatcctata aacaaagatg ttgatatgga tgttgctgct atatttacga
    89641 cgttatttcc aaccaatata gaacttaaaa gcttatttgg attttctatt aatgaactta
    89701 ctaacttagc tcctttaact ccttcatctt gcatatatct gattctgatt ttactcaacg
    89761 acattaaggc cgtttcagat gctgagaaaa atgcagaccc tataagtaat actattaaaa
    89821 aaataatctg aatcaaatta ttgggcgatt ccaacactta ccacactcct acttacatgt
    89881 ttttatatta atattaatta taaataactt taatattagt ttttattata tcacaattac
    89941 atattatttg gaaatattag ataatatatt caattaacat atattttcta actatattca
    90001 atttttactt ttgaacctat tccagcctca ctagatgaaa ctaatagctt aacaggaact
    90061 ctatttttaa gttcctcgac atgacttatt atacccacac ttagattatt actatgcaat
    90121 ctttccaagg attctataac aatttctaaa agttcactat ctaatgaacc aaaaccttca
    90181 tctaaaaaga aaaattctaa tggtgcactt ccctttaatt gtatttgaga tgaaagagca
    90241 agtgctaatg atagagatgt caagaatgtc tctccaccag atagagtatc aatgcttctt
    90301 ctttctcctc cattaaagtt atctctcatt acaaagttta gtgttgagtc aatttcaagt
    90361 gcatatcttc cttttgttat tccatccaat cgttttgatg cttccaatgc tatatatttt
    90421 aattggtttg tagaaacata ttctacaaat ttatttcctt gtacacattt atctaattct
    90481 tccaataagt ccacattgtg ttcaactacc ttaagttgtt tattaaaatc atttatctta
    90541 tctaaagagt cttttaatgt tattaactga ttttgattag ctccaatatc tttggatata
    90601 tttcctattt ctactttcaa attataaatt tcatctttta actgttcaaa gtactccttc
    90661 tttatgcttc ttccatttaa tttttctttt gatgatttta ttttaaatga taataacttt
    90721 tgttcttctt catgctctag tatttcttca atcaattttt ttactgcatc agtatctaat
    90781 aaagctcttt ttactgcata tatactttca aatttatttt ctgctaatag tttatttagt
    90841 gttgattttt ggtctttata ttgttcctta gcagttttaa gtctaccatc tatattgtgc
    90901 ttttcagcaa tgtttttttc atattctaat ctttgctctt ccaatttttt cttacttgat
    90961 tcttcttgtt ctaatatctt acatatattt tcttcaacat tatgtaaaag atttttagct
    91021 aaatcacctt tcgttatgct aataacttct ttatacttat tatccctact caatttcttt
    91081 tctacatata attctctagc ctttattaat tctatctcta tttcatgtag tttactctca
    91141 ttttctctta ttttaatatc tagtgagtct ctatttttta ataaatttga atgcccagta
    91201 cttagtaatt ctaattcttt ttcattctct cttatttctt ttacttttga caatagatta
    91261 gagactttag taatcgtctt taatcctagg tattcctgtt taattttttt acatttattt
    91321 tctaaacctt ctatttctat gtttaaatct cttgttaatt tcttataatt attcagactc
    91381 tctcttattt ttaattcttc tttttcaatt ttattttttt cttctttagc taatgttact
    91441 ttattttcag tactctcttt ttctttctcc cactcctgaa tattagattt taacaatgca
    91501 agttttcttt gttcttcatc aagttttttt aataattgac tagatggaat cccccctagt
    91561 tttcctttta catcttcaag ttctttagtc ttcatttttt ctatagataa atacccactt
    91621 actttagcat ttaattcctc tatattgttt cttatactta ttttttcttt ttctaatttc
    91681 tccagttttt ctttgacaaa agatatattt tcatcatact tagttatatc atgattttca
    91741 tgatgttttg acccacaaac tggacaaggc atattttctt ttaattcacg tctaagttca
    91801 gaggctaaat ttaaatactt tagttcttcc aattcttttt ctaagtcatc tctgttcttt
    91861 ctattatttt caagtttttc atttagtaaa tttacttccc tttcagtatt aaacttactt
    91921 tcaagtatta tctttaattc atcttgtata ctgcttttct taatttcatt ttctttagta
    91981 ttatttgctt tatttcttaa ttcagtaaca tattcagatt ttaaaagtaa atcagcactc
    92041 ttaccaggac attttttaag aagaacatcc aagtgaagtg ataaattttc taaattacga
    92101 tttacatcat tcttatcttt atcaatatat ctaactttta aatttatatt ttctgtctct
    92161 ttgagtattt cttctagttt atttagcttt tgattttttt cttccaatac gttgttgtaa
    92221 tctttctcgt attcatatgc tagaaatatt ttttgtttta attctgcact tatatttact
    92281 ttatcaatct ttccttcaac ttcttttata gatttagtaa ctgcatcttt tctggactct
    92341 gacacctgct ttactttttc taattcaact ttagtcttat ttaaattaat tccactttct
    92401 tttagctctt ttaactctct atctaataaa actaactctt cttctaattt tattgcttgt
    92461 tgcaatctta ttttttcttc actcaattta gggacttctt cattcttaat tttgtttatt
    92521 ttttcatatc tattttttgt aacttcaagc tcctgattta atattgctaa tttcttttct
    92581 aaattactta ctgcaaaact atcttcatct agcttttttt ctagattttg aactgaactt
    92641 atgtatgggt ctaccttttt agcgttacta tcattttcta caagagcaat cttttctttt
    92701 atctcactat tttttaaatc aagctcattt tttctaagtt catttttttc taactttaat
    92761 tgttcttcat atactgtttt actttcttca taagattttt gagctaggtc taaagcatta
    92821 ttcttatctt tttccaaatt ttttaactct attaattctt ggtttatttt gttgtatact
    92881 tcctctgtaa ttccatcata ttgacttaat tttgatttca aatcttttaa attttgtaac
    92941 tgtatatttt ttctcttttt cactttttct ccaagacttc ttccatattt ctcaagatta
    93001 aaaattctct ctaacatgtc tcttctatct gcacgtgata atcttagaaa ctcattaaat
    93061 ttgtcttgtg gtaaaactac tgacctagta aaatcatttg ccgttaatcc tacaacctgt
    93121 gctacttttt catttacttc cacaacttta tcagctaaaa cattttgggt attgtcattc
    93181 aatacttcta ttaatctagc attagaggtc tttgttcctg ttttgcttct agcaatggtt
    93241 ctatctacaa tgtatcttct tctagtgttt ttgcttccta tctcaaactc gtatgatata
    93301 attgccttat cgcacacact gtttatatat tcttttgtat ttctggatat atttccatac
    93361 atagcaatag ttattgcatc taagattgta gattttccac ttccagtagt tccaaatatc
    93421 ccaaacaaac ctctttcaat taatttttca aagtctataa cttgtttatc aatatagcta
    93481 ttaagccctg taagttctaa tctaattggt ctcatctgct gattcacctt cttcacttat
    93541 tatatctaga aataaatcca taagttctcc ttttggctct acacctttac taaaagaata
    93601 gaactctcta aacaactctg ccatagactt ttcttttata tctacacttt cttgttcata
    93661 acaagatgtt attattggtt ttatttctat tatgtctttt aatagttcct tcattttttt
    93721 aatttcattc tgtgatatta tctcatcagt atttatttca aaatatgacc aaatctctct
    93781 atcttgattt tgctcacata tatctagtgc ttcttctatt ccattacatt taaatacttc
    93841 tataggtttg taattattaa aatatacatc ttcaattatt ggtttttcac cagcttttat
    93901 atccactatg tatgcccctt tggtatacgc tctttcatcc ttgctatatt gaagtggaga
    93961 accagaatag tatgcattta aacgttcgga agctttttgt tgtttatgta aatgacctag
    94021 tgcagtatat tgagcctttt ctggcaaatc ttttctttca actaaaaaac ttccacctaa
    94081 ctgtataggt ctttctgatt cagtactctc tccacctatt acaaatatat gtgatacggc
    94141 tatatttata gtatcacttc taaaattttc ttctaaactt ctaaatatat ctcctacctt
    94201 ttcagaataa tttttttgtt tctcaaactc atcttcactt gaaaaaactt catttaatct
    94261 tttttcactt ggataaggta atgtagcaac cacaatattt tctccattta tattcagttt
    94321 tgtacatccc tgagttgcat atgttatttc aaacccttta tacttagctt ctatcgtagc
    94381 acttaatgga taaccatata taagtatccc ctgttcatgt gctagaggtg ttattgcaga
    94441 gagtctctct ggattatcat gatttcctga aattattaat acacacctct gaccattatt
    94501 ggcaagtctt gatacagtct gataaaatag tttttctgct tgtgctggtg gattagaagt
    94561 atcatataca tctccagcaa ttataaccat atctatttca tttgattcta ctatttttat
    94621 aaattcctca caaaactttg cttgttcctc aattctagaa tggccttcta gggacttccc
    94681 tagatgccaa tctgatgtat gaataaatct cattttttcc ccttcctcta tcttttaggt
    94741 ttaatttata tataaaaact aataacaaac agcttcatca actccaaata aatatataca
    94801 tttctctttt actgacttac cagttaaagt ttcaataata tctgcatata aatccaactg
    94861 ttttttatat ttctctatta tttgatttat attttcctca tttacaaaat ctgttttata
    94921 atccaccaaa actatttgat tatcttcttc aaaataagca tcaactatac ctcttaacat
    94981 gacactttca ttatcataaa gttttttatc gtcattgttt ataagctttt cataaatata
    95041 tatgtctttc atatttactt gtgcataaat tgatttttct ctatttataa tttcagcatt
    95101 caacattctt tttcctatgt tagaggcaaa aaatttatat attttatatg gatttactat
    95161 actagcttgt ttttctgtta ttataccttt tgaaacaaaa cctcttattt gtgattttat
    95221 atcatttaca ctacttacat tttttaagtc taaaacctcc ataactaaat ggactattgt
    95281 acctctttct gttccactta ttttttctct ttcttcttca ttttgaataa atagaggctt
    95341 ttttaatatt actttttgtt caaatatagt atttattaac tcttcttcat aattattttg
    95401 tatctttttt atttctgtaa cagatatagt agcaggtttt cttgtactaa attcatatgg
    95461 atatatatag tttaattttt cttctatttc actgtaataa tcactgtcag gattatctac
    95521 atctattttt tcaagaattt cttcaatact ttctttttct tcatcactct tcttttccac
    95581 taaaatatca ttcttattcc acaactttcc ataccatttt gaattatgtt caacattaaa
    95641 aactgcgtct aatcctactt cttctagtag attagataaa tccctatgtc ttaatacaca
    95701 aggcattatc caatctaaaa aatttttacc ttttaatatc tcgtattgtg atattgtatc
    95761 taaactttca attccatttg accatctctt tatactatct tgtatatttc tagtagaacc
    95821 agttataatc aatttttctt tagctcttgt aaatgcaaca tataaaactc tcatctcttc
    95881 tgataaattt tctatattta ttttgctttt taatgcttct ttggctatac ttggaaaaga
    95941 aatccttctc tcatagtcta caaattgagg tccataccct aaattatgat gatataaaat
    96001 actcttttta aaatcttgag tattgaaatt tttacccatt gcagagcata tgactactgg
    96061 aaattctaga cctttacttt tatgaatact cattattcta acaacatttg cattctcacc
    96121 aagtgttttt gcacttccca tatcagaact agattttttt aatttctcta taaaatttac
    96181 aaagttgaat attcctttaa gactggtttc ttcaaattgt tttgctctct caaacaatac
    96241 ttttaaatta gcttgtcttt gacttccacc tggcaatgct ccaacatagg catagtatcc
    96301 agtccttgtg tacaagtacc atataaattc atctgtactc atgtacattg acttttcttt
    96361 aaattccttt agcttaatta aaaaatcttt acttttgttt atgcactctt cacttggtat
    96421 aaactcagac tcattttcat tttgagaatc tgtaaatcca tcatactctg ctgtactttt
    96481 taaaacctca taaaaaattt tatctttact ctgaactcta atatcaatca aatcctctgg
    96541 tgtaaatcca aatataggtg attttaatac agatataaga ggtatatctt gcattggatt
    96601 atctattatt tgaagtaatg acaatattgt ttttatttca atcgtatcaa aataccccac
    96661 tccaacatca gcatacgttg gtatatccat attcatcaat tcatctgcaa acactggagc
    96721 ccatgcagat gtagctctta aaagaataac tatatctcta aattccacag gtctatatcc
    96781 atctattccc ttatcataga ccttttgaat ttttccatct tcattgactt tcattaaatc
    96841 tttgataatg tttccaacca ttctagcctc aagttgtata ttatctaaat tttcttcttc
    96901 ctctatttca ttttctttgt tattaattct atcgtcttta tcatttataa tatcatcatc
    96961 aagtttatta tctttttgta ttaggtgtat ctccgttgct ccaccaataa tagatttctc
    97021 gtctgtatcc acattaaaat ttgcacctaa atttagtctt tctttttctg tatattcaat
    97081 ttctcctata ttctcattca ttatattttc aaatatataa tttacagcat ctacaacttc
    97141 ttctctactt ctaaaatttt tgtaaagcat tatttttcta tgggaacttc cctttttatc
    97201 attatagtta ttatattttt gcaaaaataa ttctggcttt gcttgtctaa atctatatat
    97261 actctgtttt acatcaccaa ccataaatct atttggagtt tctgtatttg caacagcttt
    97321 aagcagtact tcctgcacta gattactatc ctgatattca tctataaata tttcataaaa
    97381 cttgtttcta tatccaactg ctatatcaga aggtacaata ttaccttttt catctacatc
    97441 tgtaagtata tttaatgcaa aatgctctat atcgttaaaa tctattattc ctttttctct
    97501 tttcttattg ctatattcct cttcaaatct cagtacaaca ctagatattg gtttaactat
    97561 gttataaaga tattttattt cttctctaat agaatcatta tctttattga atgtagcact
    97621 tactatactt tctaaagact tcttagtctt atctcttata gtttttgcct tttcttttga
    97681 ttcttttata tacgaaggag catcttttga aattcttttt actcctttta cataattttc
    97741 aaaggacata cttgccatct ttttatatgc ctcatcccaa gatttattac atgcttgtga
    97801 aatgtcagca atttttttat attcaacaga taacttttct gcaaaagttt ctaattcttc
    97861 tattgattcc acttctttta atgccctttc catatttaga caaagaccat taatctctat
    97921 ttttacagtg tctaatatag ctctagccca aatagactgt gaaaaatcaa aattttcatc
    97981 aatattaaat cgttctgctg agtcaatcaa ccatttttta ggttcaggag aagccattgc
    98041 aaatgaatat attcccaaga taatatcttg tacttcttta tctcctcctc tttctgcata
    98101 actttcaact aaatttaaaa acccctcatc tctttcttca tataaatctt caaatacttc
    98161 ctctattgct tcttgtttca aaatagcaca ttcagtttgg tctcctattc tgaaattagg
    98221 gtctaagttt attctatgaa agtttgattt tataacatcc aaacagaatg agtgtattgt
    98281 tgtaatacta gatttattta ataaaacaag ttggttttgt aaatgtttat tttctgggtt
    98341 ttcatctaag gcttttccta tagcatcccc tatcctctct ctcatttctg acgcagcagc
    98401 attagtaaat gtaacaacta ataacttatc tatatcaatt ggattttctc tacttgttat
    98461 catttgaatt atacgctcaa ctagaacggc tgtttttcct gaaccagcag cagcagcaac
    98521 caataaattg cattctctac tttctataac ttctaattgc tcttttgtcc atttaggaga
    98581 actcactaat taacatctcc tttcatcatt tttattatct cttcattact cttattattt
    98641 aaatttttat acttgttatc ttttatagtt gaatcaaact gacatatagc agagtaatca
    98701 caaaagtcac atgatgttcc atctttatgt ttatatggag ctatacgtat ttctccactt
    98761 aacatttctt cacataaatc ctttatagca tgccttacat attttctaag cagtttgaaa
    98821 tcctcatagc ttattgctga agtactttta cctacatttc catccttagt aagacctact
    98881 ggtattacta aagagttttt cctttctcca tctattaatg atttatccat ctctttaact
    98941 atgtgagagt cttttactac aagccccttc attctaagtt cttttaatat tgcttcttgt
    99001 atttcttcat catctttatc ttcattaaat tttgctattg gattatttat tctacaatat
    99061 agtattgcag ctggatttat atttatatct tcatcctttg cactttcaag gattgcatct
    99121 aaatacacaa gtaattgtaa ttgtagacca taatatattt ctgttagact tatggactta
    99181 ttgcctgact tataatctat tattcttata tatttatttt ctccttcttc aaattcatca
    99241 actctatcta tttgtccaat taaacttact tcttgtccat tttctaacac catttttata
    99301 ggtggatatt ttccattgtt gccaaattta acttcataat ctattggttc aaatgaacct
    99361 tgttttattt gctgactaat tatagttata gcagttgtca acatattctt taatctatat
    99421 gcaaggtatt tatacctctc agaactattt aatatatatc ctggtatctt taaaattatt
    99481 tcatcaacta ttatacctat ctttaattct atatattttt catcaatatc tctccaagtt
    99541 aaattatctt gtaaaagttc ttttgaaaac ctatctaaaa tattatgaat aaaagttcct
    99601 aaatctggtg gtgtaaatga atactcttta cgcttttttg cttttagacc atattgtata
    99661 aaataagcaa atggacattc agcatatttt tcaagcctag atatgctaag agaattactt
    99721 ttatacaatg accttatttt tttctcttca atcttatgaa cttgattagt ataactaaga
    99781 ccttttataa ctttttttgt tatagaacta tagacttcat cttttaaata gtacctatat
    99841 atatctaacc aaatactatt aatctcttct gtattgtaac catcactgtc ataatttttt
    99901 attatgttta taagttcatt aaatgtaggg gatttaaccg ttattttttt aagtatatct
    99961 ttatcagtgc ttttattttc ttccactaaa taacttttat tttctatatt tggaaatatc
   100021 ttcttaagtc ttgatataat tattgatggt ctaagcgttt taccttcatg gtctgatatt
   100081 ggatatgtta tggttaaatt cttagatgtc gaagtaagag ctttatatac aagaaactgt
   100141 tcttcaaaac tcctagtctt actatcaata tcaacttcga ttcctttgtt tccaagactt
   100201 tccctatcat tatcactcaa tattccacta tcctttgtaa tcagcggaaa cacaccatca
   100261 gtagttccaa ctaggtataa atatttcgtg tctggatttt tcattctatc cacactactt
   100321 acaagtacct ggtctatact aggaggaacc agtcctaatt catattcatc aaaacctaag
   100381 gttattaatt ttataaattt ttctaaagaa attatttcat cacccataag ttccaccatt
   100441 tggtctaata tgtccactac tatgtcccaa acttgtgagt attgatttgc aacatcaagt
   100501 tcccctttat ccttaaaatt tactattaaa ctttctatag tttctggcat atttatatct
   100561 aataaaaatt catatatata tctacagatt tcttttactc tattttttcc tcttaatttt
   100621 tcttgtaaaa ttactatagg ctctaatact ctatttttta tttcatttat tttctctttt
   100681 aattccagtt caaattcact ttcttgacct gaaagacttt gagttattct ataatcccat
   100741 ttttcatcaa accatttttt acctttaatc ccattagcca acacataatt ttctagtaaa
   100801 tttatatcat cattatcaat tcctataagt ccacttttta aatacctaaa catagtttca
   100861 tacccatatc gtctattctt catttctaaa gctgaaataa ttaaaataac tattggatta
   100921 ctttttgcct ctctcttctt atctataaag tttggtatat tatattcatt aaatatagag
   100981 tgtactaaaa aatcatatct atttaaatct cttgtagcaa tggttatatc tctatatctg
   101041 acattcttat ctctaactaa atgtacaatt tctctggcta tctcttccac ctctgaatat
   101101 aaattgttaa actcttttat ctttatattt tcagttggat ttgaatatat tttatatgga
   101161 taagcattat aaaatctttc taagtgctgt aattccttaa ctttttcaaa tctttttata
   101221 acacctgtgt ttaaatttac ttgtggcaat atttttatgc cttcttcgtt gcacaattga
   101281 gtcaattttg aataagtaaa tttcgttcta gaaaacatat cagatttact gtacccaata
   101341 tagttgatat catctacagt caaagagata ttaacacttt tagacttatt taatatactc
   101401 tttatgacat tgtactgatt tggtgtaaaa cctgtaaatt catctatata aacacaagct
   101461 ccatctaaat aactgcttaa ttcaatttta cttgccaatg aagttaacat atcttgagca
   101521 tctacataat tttcatgcaa tttcccttca aaagaattat atattttact tatatctttt
   101581 aattttaaac ttaaagtttc attatccaat tctccagata tattttctaa catttcaggg
   101641 cttatattgt attgtttcat ttctgatatc atatctgtaa ttgaacttac aaaaccagac
   101701 tgcgattttg attttgaaaa cacatttaat tcttcactta catcttcaat agctctatat
   101761 accatcattg cttttccact agaatttata tttacatctg tcaatcctcc tacttgagaa
   101821 aaaactatat tacttagtgt cttaaagcta agtaccctaa ctcttagata cttatctttt
   101881 tcttttccta aaaatagttt acttactcta ttttcagcct caaatgtgta ctgttctgga
   101941 actaaaagta taattgatgt cgtttcattt ttttgagctt cttttttaat ctcatctagt
   102001 atataggtgg attttccact tccacttcta cctaatacaa acctaagtcc cataaatttc
   102061 cgcctccaaa atactataat ctatttcttt gccattacat aacttattaa attcacaata
   102121 cttgcaatgc tttttatttc tatagcacat acgttcttct ttattatata ccttttctat
   102181 atcattgtat agattgtctt cgtatgtatg ttcttcataa ttagtgtttt ttatcatact
   102241 tatataattt tgaattttat ttctatttgc ttcatgtttt tcttcatcat ataaaatggt
   102301 aattggtaag tcatcaaatt caggttgata atatctcata tttatatctt taacatttat
   102361 gtccattttt aaaactttag gtattacttc ttttgctaaa aacatataaa caatagtttg
   102421 tattctattt tctacgtgtt tatagtcaat ctttttagac tcggttttcc aatcccatat
   102481 ttctatattg ttatttccaa ttacaactaa atcatacttt gcctgtatta tatccccatt
   102541 taatgtcatc cttacttcat attctggcaa atacgttttt cctttttcaa taggaacaac
   102601 tttttttata ttttccatcc actttaaaaa ctttttacta tttttatcat cctcattaaa
   102661 atagattcca agaggtatat tgctaaaata tctttcacaa atcaaatgaa aatctcttcc
   102721 tgtttttaaa gtttcataat actctctact accaacatca tcatttttcc aatttattct
   102781 atcaatatat ttatatttaa atttcaatgg acatgattta tatgtgttaa ttgaattttg
   102841 actataagaa aaatatttta atttatcgtt cactcatatc acttctctct taactttatt
   102901 ttttagtagt tacttactgt acatattgtt ttatcccctt ttagatatat ttcaagcaat
   102961 cttttaataa cttattatat attaatttga ccttttttca ataatatgtt gattaagtct
   103021 gcttaaatat atacatggtt tttgctcttt tctcttattc cctatatcac tttcatctct
   103081 ataagcactt cctgaaagta ctaacatttc ctttgctcta gtaatcccta catataaaag
   103141 tctaatcttt tcatttatag aatcaatctt cgttttatga gcataatctg ttgaaatact
   103201 tccttttaat atagcttcaa cttctgattt aataattgcc atagggtttt tatatttttc
   103261 ttttagatac catttatcac tctgaaactt ttgattaata ttgtcaggga aattatattc
   103321 aacaagacct agtaaaaaca cacaatccca ttccatcccc tttgacttat gatagttaca
   103381 tagtgttata ctaccgggct ctggttcata tccatttatc tcatatacca catctattat
   103441 atgattaaac accctgttct ttacatcaaa caataaatta tatacatctt ctaaatccat
   103501 atttatattt tctgatacta gatactttat ataaaaagat atataatcca gtacagcttt
   103561 atcttctttt tctaaattaa gttttttccc aataaacaaa actaagaggt ctaaccttat
   103621 tattggatat tctaatatct gttttataat atctattcct cttttaaaac ttaagtatat
   103681 atcactttct tctttaaatt ctattaaatc attatctaac ttactaaatt caaaattata
   103741 aataatttct tcggtagaat agttttttaa ttcttctaaa aaatacttta tttcttcttc
   103801 attatcagta tctataaaaa ctttattaag tacaactatt aacttttcta catcatcaca
   103861 atgcaacata aaatcaataa tatatgctat attgtttata accttccttt tattcaaaga
   103921 atttggtcct aattcttcaa aggttagatt attctctcta agttctttag caacttgtgt
   103981 cacttgttca ttaaatggaa ccaatatacc tatactttta tctggatatt tattttttat
   104041 acccttaacg tatctagcag tttgctctat ttcaattttc caactctcat accactttac
   104101 atttatatta taatgttctg gattagggtt ttctttatat cccattccat ttggcacagt
   104161 ttttatttgc atatcttcaa gagcatgtct acattctttc tgtctaaatt cctttgtaac
   104221 atacttgaca agagtgtttg ctaaatcaag tatatctttt gaacttcgat ttgacatatc
   104281 catcctataa cagaaatctg cactatttat aaattcttta aaaaatttag ggtctgatga
   104341 tgaaaatgtt cctgttatac tctgatttat atctccaact ctaactaggt ttttattttc
   104401 gctagatata atttttatta tattaccttg aatttcattt gagtcctggc actcatcttc
   104461 aaatatatat ttgtatctag tttgaaactt ctttctaagc ccttcgtcta agcttaaagt
   104521 tttttctgct aaaattaata tatcatcata atctagcaat ccattttgct ttaattttct
   104581 atcatacaat ttatatattg gtaaaataat tttaagcatt cctttgtgtc ctcttgacaa
   104641 tatttcttca agtttatctg gagaaattcc cttatactta agttcactta ttgcatttcc
   104701 tgcaaattca aaaaaacctc tttcccaggc ttcaagagtt atttctttcc agcgttcatc
   104761 tttttgctcc tttaaaaacc atctaaaagc tctttctcca ccttcaaatc taaatctact
   104821 tatacattca tttaatataa tacttttttg caaatcgtca gctatgttaa aatcctcact
   104881 taacattaca acttctggct tttcctttat tatttttaca gccaaactgt gtatagtcat
   104941 tacttcatac gaattagtat catctatttt attctcttca agaatctttc ttattctacc
   105001 tttaaaatta tttacagcac tattcatata tgtaagaatt aatattttac cacccttatg
   105061 tttctgctca agtaatagtt tagctactaa atttgttatt ataaatgttt ttccagcacc
   105121 tggtactgct ggtactgcca tagtaccatt atcgtaattt attattggta tctggtcttc
   105181 tctataattt atattaatca taatttttat ctcctttatg tgagattttt aaaagtatac
   105241 tatataatat actctcttgg atataaccat taatcgcata ttcactttta aatgcataga
   105301 ttttctttgc atttaataac aaattatata ctgtattgta tagatagtat tttttataat
   105361 attcttccat ttcatttgta taaatctttt tttcttcaaa agattttcta agtacaatta
   105421 aattacttat atctttttct attttcatat tccaagcatt acttcctata tctacccata
   105481 tttgtatact tctatctata tttgatgata tatatgaata aggagtagtt attactattt
   105541 tgtctttgga tttcaactct tctatatcga caacagagta ataatcgttt attgtacttt
   105601 ttaaagcttc tataaatatc ttttcttttt tcatcccaag tagacttaaa ctttcactaa
   105661 aagactcact ttcagctatt atctttttac ataacccaac attcttttta ccttctttta
   105721 aatttaacat tttatctatg taaaattgag ttaaaaattc atgtattttt atatcactct
   105781 tctttttttc ttcaatatac tgtaaaatct cacgatactg actatgttca tctatattat
   105841 caatacttaa atgtcttgtt tcattaaaaa tcttaaatgc ttttatccta tttatttcta
   105901 aaagagtttc taaaaagctt aagtattcat catcttttat aaaatctaaa tatccataaa
   105961 atatacatgt agccactacc aatgcattcc catatggata atccacagct tttttatcct
   106021 tcttagtact aaaaacatct atattttttt ctatcaaaga atctctaatt tgatgctcca
   106081 atatactatt gtttatagga gatataattg ctatatctct tggagataca tcttcttcta
   106141 taagagatat aactttttca cttattaaat ctaacatttc attatataat tggcttgatt
   106201 gatttaattc tatatttgca ggtaataagt ataaatcttc tattctaatc tccttctttt
   106261 taatattttc ttctttaggc ttaatttttg atattatttt ctcatttata tagtctatat
   106321 ccacattatt aaatacagaa taatccctac tataatcaaa atatatataa ctttgtaatg
   106381 tattatccaa tgcttctgtt ataaaatcaa cttctgcatt acttgagctt tcaaggctat
   106441 ccacaattat atatctcact tctgtaaata agttttttaa ataaaaatca tcattaagta
   106501 ggtattgatt gtataaatat atgcttaatg cattatctaa aattgaattt gatagtaagg
   106561 aatttatata atatgatata atttcatcca tttgagtgta tgaaaatcta tatatactat
   106621 ctttattttt ctttgatgaa tatatttttt ctccaataaa atcaaaatca attaaattaa
   106681 atgcagactt atttatgtta gtcattatac tatttgctat ggatttattg gttcctgtaa
   106741 tatcttcaaa atatccttct tgtatccttt tttccttaac tttttgcact attaaatatt
   106801 cacttaaact atttgatata aatgttggag atacaacctt tgtacttatt tcatcacatt
   106861 tttctattat tagtggccag tatttaacta attctttctt cacaaaactt atatatgttg
   106921 ttattttcag ttcttcacta taatttaatc ttagagacct ttcatatcgt atctttgtgt
   106981 tattgtttgg tactaacaaa agaatacacc ttgaattatt accagtttta tctaataatt
   107041 ctctatattt atctaaagct tcatttccat attcttttaa actacttttt tcaaataaat
   107101 attccatata atcaccttcc tcaattaaat taagtgtata ttatctaatt atatcatatt
   107161 gtgtatttta caaaacacaa tcaaattatt gcatttaact acaaaaaaag actgtatata
   107221 tacttgcatt cacatgtgca aatgtacata cagtcttact attgatacac ttatatttca
   107281 ttttttgact ttagtataaa tttacaacta taaatttaca ttaagttttt aaatattttt
   107341 atttccattt attttaatga atcttctatt cctttaccta tttgtgtacc catttctttt
   107401 acttttgcag atgagtttat taaactcata tcattttcat tatccaaaaa tgcacattct
   107461 acaagtacag ctggcatatc attacttctt aaaacgtata aataatcttt tgtagaatca
   107521 ctaggaagta ctcttgtttt tatacctcta tttgtaagtt taaatttctc taaaattcta
   107581 tttaatatat tggtagctac agttttagta atccctccat ttttatcttt aagcttataa
   107641 aatacttcta caccatgacc ttgtttatta gttgagccat tataatgtat acttgtaaat
   107701 aaatctggtt tcaatgaatt agatagagca gttctatttc caagagataa agtcttatct
   107761 gtgtctcttg tcattattac attgaatccc tttgaacgta aatattcagt tgttgcaagt
   107821 gaagtattta aattgtaatc tttttcaatc attcctccag atgaatttcc aggtgctgaa
   107881 ccaccatgcc ctgggtcaat cataactgtc ttgccaccac tatttcctgg ttctgttgga
   107941 gtcgtattgt gtttagataa tgatgatgct atagagctca ttaccttaga agtcaatcct
   108001 cctcctattt tgtatacctt attagctgat ttagcttcta aattatcttt atggtatgca
   108061 gatacatacg tctttggtgt tataagtatt ggagatttta agttcgcagc caatggacct
   108121 gctgctaaag catcaacaag tacatctgat ttagctacta aaacagcctc taactcatca
   108181 tctgtataga atttttttat tacgtttgca tttgtttcgt gtctatctgc tccgtatact
   108241 ctattattag taacactatc tttagttatt ccatttacct tatttataac atttgttgat
   108301 atcatttgag gaccacctat aaaataagca ttttgtaaat cctcactctt taaccattta
   108361 tatgtattgt ctgtaatact atctttatca gttaatataa ttggttgctt gtcttgacct
   108421 gcttttgctg ctatagtaag tgcatccact tctccgccat tagcatttgt tatgtatact
   108481 ttttccacat catgattttt atctatttcc tttgctatca ataaagatgt ctcatatcta
   108541 ttagaaccat ttaaacgtgt ttgactagca tttacagttg atttaatttg attagcagta
   108601 gttttagaaa tagcattctc atctccaatt ataattatat ctttagggtt taggcgcttt
   108661 aattctgatt ttacactatt aggtacattg tttttttcaa ccaaaagtat tggtgcatta
   108721 tatgtggttg ccagtggagt tgatataatg ccatctatac ttacatcccc atttattata
   108781 actaccttat ctgaaccatt tttccagccc tctttactta ttttaactgc tgtttcatat
   108841 ctatcacttc ctgtaagttc tgtaataggt gaagaagttt ttctcaaata tttatttaca
   108901 tcttttctag aagttatatc ttcaattata cattcctgtg ttacattata gtcttttaaa
   108961 tttaatccca gcttttctag gtcttgtctg tttaaagttt taggattatt tttgctgttt
   109021 attgttatcc cttgtagttc ccaaacattt agatattctg ggtcttggtt ataattgata
   109081 gttccttcat atgccccagt tgattgattg taatttagag ttgtattcaa tggcatgtca
   109141 tagcatttat actttaatgt aatgctatct gcatctggtt tttctttaaa tttgacacta
   109201 acttttgatt tgctacctaa aacgatactt tttttctcta agtttatatt taaatcagcg
   109261 ttattttcca tagcattaga ttcagtagca aatgccattg taggtgcgac gattgccatt
   109321 gaaagaatcc ctgttgttaa taattttgta tttcttctca tattaatctc cttacttaac
   109381 ataaatttct actttatttt taatctttta acttttgaaa atatgtcaaa gtgtatctaa
   109441 cccaaaatat aaataatata taagtaatat ataaaaataa acttataaaa ctcaaaaaat
   109501 aaaaaaatta aatatattta atacatttaa ttatctatac tatatcattt attgacataa
   109561 aaatccacaa aaaacacaca aacacacaat aaaatatagt aattaagctt ttattgtgta
   109621 ttacaaatga atactccaac tcttgacaat taactgtagc cttaagatat gtaaattctt
   109681 catataatat aaatcctttt acaattaatc ataaaaagac tgtatatacc tcatttcgca
   109741 cttatatgca caatgtatat acagtcttta ttactattat aattacattc ttatcttata
   109801 gctttagttt aaatttaaaa ttgcagatta atttatttta aatttttcta tgctctcatt
   109861 gtagactatt ttaatgaatc ttctatgcct ttacctattt gtgtacccat ttcttttact
   109921 ttatttgatg tattaagtaa actcatatct ttctcattat ccaaaaatgc acattctaca
   109981 agtacagctg gcatatcatt atttcttaaa acatataaat aatcttttgt agggtcagtt
   110041 gatagtgttc ttgtttttgc acctctattt tttagattaa atttctctaa gattctattt
   110101 aatatatttg tagctacagt tttagtagtc cctccatctt tatctttaag cttataaaat
   110161 acttctacac cattcccagt tgtatcagat gcattatagt gtatactcgt aaataaatct
   110221 ggtctcaaag agttagataa agcagttcta tttccaagag ataaagtctt atctgtgtct
   110281 cttgtcatta ttacattgaa tccctttgaa cgtaaatatt cagttgtcgc aagtgaagta
   110341 tttaatgtat agtctttttc tctaataccc cctaatggtt tacctgttgt tccagtgtcc
   110401 gaaccaccat gtcctgggtc aatcataact gtcttgcccc cactatttcc tggttctgtt
   110461 ggagttgtat tgtgtttaga taatgatgat gctatagagc tcattacctt agaagtcaat
   110521 cctccaccta ttttatatac cttatttgcc gatttagctt ctaagttttc tttatggtat
   110581 gcagatacat atgtctttgg tgttataagt attggagatt ttaagttcgc agccaatgga
   110641 cctgctgcta aagcatcaac aagtacatct gatttagcta ctaaaacagc ctctaactca
   110701 tcatctgtat agaatttttt tattacgttt gcatttgttt cgtgtctatc tgctccatat
   110761 actctattat tagtaacatt atctttagtt atatcattta ccttatttat aacatttgtt
   110821 gatatcattt gagggccacc tataaaataa gcattttgta aatcctcact ctttaaccat
   110881 ttatatgtgt tgtctgtaat actattttta tcagttaata taattggttg cttgtcttga
   110941 cctgcttttg ctgctatagt aagtgcatcc acttctccgc cattagcatt tgttatgtat
   111001 actttttcca catcatgatt tttatctatt tcctttgcta tcaataaaga tgtctcatat
   111061 ctattagagc cctttagacg tgtttgacta gcatttacag tcgatttaat ttgattagca
   111121 gtagttttag aaatagcatt ctcatctcca attataatta catctctagg atttaaacgc
   111181 tttaattctg attttacact attaggtaca ttgttttttt caaccaaaag tattggcgca
   111241 ttatatgtag ttgctagtgg agttgatata atgccatcta tacttacatc cccatttatt
   111301 ataactacct tatctgaacc atttttccag ccttctttac ttattttaac tgctgtttcg
   111361 tatctatcac ttcctgtaag ttctgtaata ggtgcagaag tttttcttaa atatttattt
   111421 acatcttttc tagaagttat atcttcaatt atacattcct gcgttacatt atagtctttt
   111481 aaatttaatc ccatcttttc tagttcttgt ttgtttaaag ttttaggatt atttttgctg
   111541 tttattgtta tcccttgtag ttcccaaaca tttagatatt ctgggtcttt attataattg
   111601 atagttcctt catatgactc agttgattga ttatagttta aagttgtatc caatggcata
   111661 tcatagcatt tatactttaa tgtaatgcta tctgcatctg gtttttcttt aaacttaaca
   111721 ctgacttttg aggt
//
LOCUS       DAGSRM010000002       111174 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.002, whole genome shotgun sequence.
ACCESSION   DAGSRM010000002 DAGSRM010000000
VERSION     DAGSRM010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 111174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 111174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..111174
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.002"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(940..1449)
                     /locus_tag="MJE33_000103"
     CDS             complement(940..1449)
                     /locus_tag="MJE33_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBY9646087.1"
                     /translation="MVMFLFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLSPI
                     YSNNTVIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRG
                     GKCEELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLNEEFATILDISPNEVS
                     SYISSHIPQ"
     gene            complement(1660..3081)
                     /locus_tag="MJE33_000104"
     CDS             complement(1660..3081)
                     /locus_tag="MJE33_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646088.1"
                     /translation="MENYFDKQAKELYNKASEIINTHSMLKANRANEKFDIDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMSREIRFDISSPTGVNFKGAKYIIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKELKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDGEEDDSDKYGDIEI
                     DYSPERTHDIWDESNEIDENTLREFTEKFINSSQKGKVPAYLESMISSLKNSKGELPW
                     NLYLNRIMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKSEIAVALDVSGSISDEEF
                     KQAIKEVLSIVKNYNHEITIIECDNEIRRVYKVRTEKDIKDRIRIGGGTRFSPVFEYA
                     NNKKINLLVYFTDGKGENKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVKTN
                     DEILEINDVRNDGYSMNSQAPIL"
     gene            complement(3085..4215)
                     /locus_tag="MJE33_000105"
     CDS             complement(3085..4215)
                     /locus_tag="MJE33_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBY9646089.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LIVIDGNLLKEGEIGGLPTIESYVGVNSNGYKTEKKTTVYAVHNKLREIDEEISKSKT
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESDHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKEKNNSIPRAVFLNVVKGNVGKVIAEEFISFIESNSEPLRSYEDV
                     FLGESIDESIVERVKNESHTRLYLSAMNILKDLELNIKNDKYESNHYINRFIEFLKMY
                     PVDLMIGIMKDIRNSYIEVYKKAIENEEFVKSYFESYSLIRG"
     gene            complement(4227..5030)
                     /locus_tag="MJE33_000106"
     CDS             complement(4227..5030)
                     /locus_tag="MJE33_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646090.1"
                     /translation="MEKLRVIEMIKNNPNIIATIDNPTDEMKLLAIKENGLVLEYINN
                     PTREMQELAIDNNIRAIKFIDNPTEDMMIKAVNEGWSILDYIKNPTDKVIEMAINQAG
                     WAIKYAKNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYNAE
                     LIAIKNNEAAISFITDLDKNKMLEFLKVNILVIKYILKKVSKDELESVLKEVLSKEDV
                     EEKYIRDFLNCSVIDRNSESIPIDKIMFIYKYGSKTAKKIAVDEKLKIM"
     gene            complement(5412..6293)
                     /locus_tag="MJE33_000107"
     CDS             complement(5412..6293)
                     /locus_tag="MJE33_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HBY9646091.1"
                     /translation="MKITEGYMPFKGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIEELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSAKLWEKEQKRRISYMSEVDQKALLDAV
                     NTGDYSSKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGAIDLCSPYIAKTMYDRIPNSKWELFEYSRHMPFVE
                     ENEKYIKVLTEWLNAND"
     gene            6749..7723
                     /locus_tag="MJE33_000108"
     CDS             6749..7723
                     /locus_tag="MJE33_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646092.1"
                     /translation="MNNINSLKVIKLSLGCSLAIFIAWLLKLEYSMVAGVIVLLTVKD
                     TKRETLKGSIGKIYGFLLCTIFSYLCFNILGYNLTSFSIFIFIIIPLCFLLNIQDVIA
                     MCVVIASHYYLQGETSIKWILNEAGIFAVGTGIGVLINMYIPTNIHKIHEVQKKLQEE
                     VSIVLIDIADIIVNPKKENGYSRDLYTLNSLIDSSISETYDNINNTLLSDTRFFLEHM
                     DIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGYTEFEAETGNLLLEELKRLM
                     ISMKNQPLPVDRTEFENRAILFLCLTELKQFLVNRKHAQMLRDNNFYK"
     gene            complement(7839..8900)
                     /locus_tag="MJE33_000109"
     CDS             complement(7839..8900)
                     /locus_tag="MJE33_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBY9646093.1"
                     /translation="MKKIRKLATKLLITTIVLISGMSMTVYGMTAKEVTAKTPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDIIYKSLSHPLPFDKTAYPKVGAIVQTVKPEQTQKPAPPGKHTMLP
                     HQEGAKAVANFGKKGEDINEKHVMYEGVRCSVVNSSVNTITFTGSESNLDPSNLTIAV
                     IGFMNTPYGMFALAPEGKPVEYPFKYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIE
                     ITDEKANIGLGQIMYVRYE"
     gene            complement(9511..10362)
                     /locus_tag="MJE33_000110"
     CDS             complement(9511..10362)
                     /locus_tag="MJE33_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBY9646094.1"
                     /translation="MSCILKLKQIYEDLTEVDKKIADYILNNTEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDIAIAISYSGESKEVIKSIENAKLKGCKVICITKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNINNA"
     gene            complement(10379..11299)
                     /locus_tag="MJE33_000111"
     CDS             complement(10379..11299)
                     /locus_tag="MJE33_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HBY9646095.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDEIQGDRIEIAKFATICLKA
                     VIEGDNTASKIFDDAAYELSRLIIGLEHHFEQGTKIKVSYSGGVFKSGDLILEPLKKY
                     LNKTRFGIVRPVLTPDLGACLLAKKKYYSK"
     gene            complement(11409..12323)
                     /gene="murQ"
                     /locus_tag="MJE33_000112"
     CDS             complement(11409..12323)
                     /gene="murQ"
                     /locus_tag="MJE33_000112"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBY9646096.1"
                     /translation="MLIKTLENLVTEGRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVNIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAIEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNVKYDVAEKFLEEADNSVKLAIFMIKS
                     GLDKDSAKSILDRQEGYISEALKSIEKL"
     gene            complement(12422..13519)
                     /locus_tag="MJE33_000113"
     CDS             complement(12422..13519)
                     /locus_tag="MJE33_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBY9646097.1"
                     /translation="MGRLGISIYPEKTTSKEIYDYIDKAAENGFTRIFSCLLSVKDTK
                     ENIIKKFKSINEYAKFKGFEVILDVNPRVFGELEISYKDLNFFKKTKADGIRLDMGFT
                     GSEEALMTFNPQNLKIEINMSNNTNYIDTIMDYQPNKENLIGCHNFYPHRYSGLNLEH
                     FIKCTERFNKYGLRTAVFVTSQNETTFGPWPVTDGLPTIEMHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DSLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGYIRKEELFILDYIKPWQKFNFKPSTK"
     gene            complement(13752..15098)
                     /locus_tag="MJE33_000114"
     CDS             complement(13752..15098)
                     /locus_tag="MJE33_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBY9646098.1"
                     /translation="MLDKFTEFLDTKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYLKENAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPDLLKIVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIIIYYPFVKMYDKRLLHEEKVEEQEAY"
     gene            complement(15187..15495)
                     /locus_tag="MJE33_000115"
     CDS             complement(15187..15495)
                     /locus_tag="MJE33_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY9646099.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGAKNGIDIDVLAVGTQEFENE
                     VKNGWDIAMVAPQVRHRFDYLKAFADEVSVPCALIQAQAYSPLGGPKLLKQVQELLSK
                     "
     gene            complement(15757..16155)
                     /locus_tag="MJE33_000116"
     CDS             complement(15757..16155)
                     /locus_tag="MJE33_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646100.1"
                     /translation="MSNNKKKKRKKNNNYKNAKSTKSNKNTKKQMEKNQRTDYNIPTI
                     SFKSGLILIVSSLVSTVVFPLLLSIFGIDNRFGVVIGNSLITSFAVAYTRYFVETKRG
                     FSLGFFRLYILFALSFAIIGYFWIYKGIHI"
     gene            complement(16157..17317)
                     /locus_tag="MJE33_000117"
     CDS             complement(16157..17317)
                     /locus_tag="MJE33_000117"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HBY9646101.1"
                     /translation="MITMYSVGLMSGTSLDGVDAVLAEISGNGRNTKVKQIEFITLEI
                     PKDIKDEIRKCCIEEESSVDLICSLNFKLGYLFSKAVKSVCHKANFHIANLDFIASHG
                     QTIFHIPRSYNNFVSSTLQIGEPAVIAYETNTKVISNFRVMDIAAGGEGAPLVPYSEF
                     LLYSDNNKNLALQNIGGIGNITIIPKSCNIDDVFAFDTGPGNMIIDGVCQRLFNRKYD
                     KNGYFASKGKINEEMLKDLMSHKYLSQAPPKSTGREVFGQVYLDNMLNKYKHVDKYDL
                     IATVTMFTAKAIYYNYKNFILPKVNVDTLLIGGGGAHNLTLIGYIKELLLEVEVLTQD
                     EYGYSSDAKEALAFVILGNETLNNSFSNVISATGAKNKVILGNITPKPFGGK"
     gene            complement(17748..18767)
                     /locus_tag="MJE33_000118"
     CDS             complement(17748..18767)
                     /locus_tag="MJE33_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY9646102.1"
                     /translation="MLVKYLKGRSKYIALPLVVVSIINIYLFAIDIFKNKYSELIYLD
                     FLVLFIIMVFFVIDYINFRNSYTNLYNCIENSGEIDSYLVDGQSFEENLIKDIIENLK
                     IKNNSDIETYKQSLKELDEYIAKWVHEIKIPISSLSIITDRLSSIEDSLDIKNQVAKI
                     NFLVNSILYSSRSNTMFEDVFINKFNLEKLVKMSIKNNSFLLIKNNVEVSLNELENDV
                     YTDSKCMSYVLDQIINNAIKYSKEVGKIEFNSKKLENGVVLSIKDFGIGINEEDISRV
                     FDKGFTGKNGRNQLYKSTGMGMYFVKKMIDSLGHEIEVCSENGSYTIFNIYFYDISDY
                     LSLDS"
     gene            complement(18761..19441)
                     /locus_tag="MJE33_000119"
     CDS             complement(18761..19441)
                     /locus_tag="MJE33_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY9646103.1"
                     /translation="MYRILLVEDDIDLSKEIALALEKWGFKVELIDDFEVVLDEFIDK
                     KPDVVLLDVNLPLYNGFYWCEKIRAISNVPLIFLSSRDSDMDLIMGINNGADDYITKP
                     FSIEILVTKINGIIRRVYNYSDSNSILYCEDLMFDVGKGIIKHKYKDKSIELTKNEIK
                     ILTLLLKNKNRVVSRESLMMTLWDNDEFVTDNALTVNMNRLRSKVKELGFDDFIKTKK
                     GIGYIIQC"
     gene            complement(19593..21496)
                     /locus_tag="MJE33_000120"
                     /pseudo
     CDS             complement(19593..21496)
                     /locus_tag="MJE33_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(21497..22249)
                     /locus_tag="MJE33_000121"
     CDS             complement(21497..22249)
                     /locus_tag="MJE33_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9646104.1"
                     /translation="MKEILKIKNISKDYGIKGFKTNVLKNISLTVNEGDFISIMGPSG
                     AGKTTLLNLMSTLDKQTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMK
                     LMDNIALPLALGKKKSKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLI
                     TNPTVIFADEPTGALDSKSAYELLESLEKINRENNATIIMITHDPLTASYSNEVYMIN
                     DGNIKCKLNKGNSRKEFYGKIMDMLASMGGEM"
     gene            complement(22565..23719)
                     /locus_tag="MJE33_000122"
     CDS             complement(22565..23719)
                     /locus_tag="MJE33_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY9646105.1"
                     /translation="MINKAIKFKFIIFYFFSYIIFAFASTKFTPFLSKLGYSAFERGI
                     ILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYLLL
                     VALSGGLLNTCCSLYDTWIIGCGDNINNYLSFIKTFGSIGWAIGSMVASYLILRFSYF
                     GLSVAIGLIVVLLIINILLLPDIEKINKKINVTNKDIIELLKDKKYILLVCILFLLYS
                     MVVANNCTVIDKMIALNATNSQISLKWSLQSLLEIPTYLAGSYLLKKYSSLNLLKLSA
                     IMITVQFLLFALTNDSNIIIILCVFQVFSTPLILITSKRLIFEVSPKKLRSSSQLIAL
                     SIFMGGSFLIIPTVAGFLSINIGYNYTLMVLSLFGCLAYCLIVLLNYLQE"
     gene            complement(23722..24189)
                     /locus_tag="MJE33_000123"
     CDS             complement(23722..24189)
                     /locus_tag="MJE33_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBY9646106.1"
                     /translation="MFFKRKKEAITINSYIDGKIINLGEIPDKVFSTKVMGDGFAMIA
                     KDNILKSPTKGVISFIAQTNHAIGIKLQNDVQILIHIGLDSATTKKENFKLEVKQGQE
                     VNMGEKLVEIDNKLLENTLYIPLVIVENPNTVKYHLSNLNQEGNSGIKVINFD"
     gene            complement(24203..24691)
                     /locus_tag="MJE33_000124"
     CDS             complement(24203..24691)
                     /locus_tag="MJE33_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646107.1"
                     /translation="MITNKIKNITAVLKDENIFLSVVMVCFLILPVLYIVYLELTSLF
                     TNISFSQLLNNDVNASLNLISNFSAVYSAYVIYNIKKNKEIKENMIAFYLVLLGQIFQ
                     IYTITIVIMFTYIYKFVGIKQIISSIKELQWGKSYKALFSSIAVCLISLFVMIIRIKL
                     IM"
     gene            complement(24705..26036)
                     /locus_tag="MJE33_000125"
     CDS             complement(24705..26036)
                     /locus_tag="MJE33_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBY9646108.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVAKFPIESIKLYDNND
                     ERQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGIAYGLRTIGPIIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARVLGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKLQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPTTLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKNGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMVEIPCLVGKDMVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            complement(26050..27615)
                     /locus_tag="MJE33_000126"
     CDS             complement(26050..27615)
                     /locus_tag="MJE33_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003233606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBY9646109.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSPATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYIHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHIQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFGAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFVAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYVVQVVVGII
                     FSFIWFFVFTFLIKKFDMKTPGREEDSEETKLYTKNEYLETKDEKGNKLSKASQQAYE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKAVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            complement(27785..28570)
                     /locus_tag="MJE33_000127"
     CDS             complement(27785..28570)
                     /locus_tag="MJE33_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBY9646110.1"
                     /translation="MRLEEMIFLNYNKLNDTDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDALCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTMLYFILIELLFIKYSIYKRHRMITEGIDCKL"
     gene            complement(28737..29936)
                     /locus_tag="MJE33_000128"
     CDS             complement(28737..29936)
                     /locus_tag="MJE33_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HBY9646111.1"
                     /translation="MKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVDDFNYVYNV
                     VKTGYPYLGVNKRLNNIDWLANKDEYTKRIKNTENDEEFIEELSSILSDLNNKHTEVI
                     DNKKRYELFKKSYSNNDWYDFLNDKKVVDRYDSMNLKIKMPDDIFVKKELILKDVIKG
                     QIGYMYLPSMSSKNGSINNDLKIIGDYINTLEKHKALIIDIRGNLGGSDRYWQGIVSK
                     LIKNDVKINGYRIYRNNNEIVKKYTNARNIKLNPVENLPASVKENAPKEIIKDFNDFE
                     DTSYTIKSTDDSKFKGNIYLLVDRKIYSSSESFAMFCKENKFATLVGETTGGDGGCID
                     PVLFNLKNSGLIVRMASCMYLNKNGICDEEFKTIPEFKVKDCKRTADFKDDNCIRKAL
                     ELENINY"
     gene            complement(30458..31795)
                     /gene="xylA"
                     /locus_tag="MJE33_000129"
     CDS             complement(30458..31795)
                     /gene="xylA"
                     /locus_tag="MJE33_000129"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HBY9646112.1"
                     /translation="MSEIFKGIGQIKFEGVKSDNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNITDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKVNMEKTGIKLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFTGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKMNIEANHATLAG
                     HTFQHELNIARINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
     gene            complement(31891..33441)
                     /gene="xylB"
                     /locus_tag="MJE33_000130"
     CDS             complement(31891..33441)
                     /gene="xylB"
                     /locus_tag="MJE33_000130"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HBY9646113.1"
                     /translation="MKLEYVLGVDIGTSGTKTVLFDKLGNTIKSCTYEYPLIQEKSGW
                     AEQDANDWWKAVVESIREVVQSSNISSECIKGIGLSGQMHGLVILDNEGKTLRNSIIW
                     CDQRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYKNTNKILLP
                     KDYIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEITG
                     YVTRDVASLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPK
                     IDKEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLGEDVYEIIN
                     NQVSKVPTGCNGLLYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYS
                     LKDCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAIL
                     ALVGAGIYENIQKACDAIIKTSTKLEPISENVDTYNIYHRLYKRIYKSLKDDFKLLDE
                     VVNAKINN"
     gene            33649..34809
                     /locus_tag="MJE33_000131"
     CDS             33649..34809
                     /locus_tag="MJE33_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBY9646114.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDIGYDYIFSSLSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKQLYYEDNQNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            complement(34965..35369)
                     /locus_tag="MJE33_000132"
     CDS             complement(34965..35369)
                     /locus_tag="MJE33_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY9646115.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANNDSRIKIISGLNLGMVIESAF
                     LDTKELAAKLEKIGKDNISMVEQNASLLSDDE"
     gene            complement(35390..35872)
                     /locus_tag="MJE33_000133"
     CDS             complement(35390..35872)
                     /locus_tag="MJE33_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY9646116.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVEAGFKI
                     HNINVGNMSSRKDTTVLRPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            complement(35892..36701)
                     /locus_tag="MJE33_000134"
     CDS             complement(35892..36701)
                     /locus_tag="MJE33_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBY9646117.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIVVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            complement(36694..37473)
                     /locus_tag="MJE33_000135"
     CDS             complement(36694..37473)
                     /locus_tag="MJE33_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBY9646118.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSVI
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQEQQYTVVGGMDEDE"
     gene            complement(37473..39551)
                     /locus_tag="MJE33_000136"
     CDS             complement(37473..39551)
                     /locus_tag="MJE33_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBY9646119.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     NFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHTWENIFSGVSYEGGKTYEVECPLEEIPIFLKQDSSYNFKE
                     LKNILGEVKS"
     gene            complement(40350..40871)
                     /locus_tag="MJE33_000137"
     CDS             complement(40350..40871)
                     /locus_tag="MJE33_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBY9646120.1"
                     /translation="MKRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAIDLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIPHVRQFDEYLKNDYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCNELMDKINDIQMVDGKGIFIEELEKLLKEYNLE
                     IPIVGFDELREDK"
     gene            41037..41603
                     /locus_tag="MJE33_000138"
     CDS             41037..41603
                     /locus_tag="MJE33_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBY9646121.1"
                     /translation="MDTKKVTFSSMCIVINIVLGMVISMINIPLLFLDTVGTVLGAVV
                     LGPFWGALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISKKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKNTSSSY"
     gene            complement(41820..43883)
                     /locus_tag="MJE33_000139"
     CDS             complement(41820..43883)
                     /locus_tag="MJE33_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HBY9646122.1"
                     /translation="MKAGEIEFLSYLEGSNKSFVVPVYQRNYNWKKEQCKRLFDDLED
                     IIDSGFRTHFLGSVVSIYSMGKEYLIIDGQQRVTTVSILLIAMCQIIKEFGESKGIIT
                     TEEQIKECYLINKHSRDKDGKIKLKPIAEDRKAYYELFEEEMNTDRNSNIVSNYLYFY
                     NRIKEDNISIDDLFDAIQKLTIVEIELKNGEDDPQLIFESLNSTGLDLSEADRVRNFV
                     LMGRSSKLQEEFYNNYWYKIEKNTSSNVSSFIRDYLTIKERSIPNKNKVYFSFKRYVS
                     DNGIDIEILLKDLLKFSNYYREISYSCTDDNDVNKVLKRINKLEIFVSYPFILELFDD
                     YNEGIINKKVLVESLNLVECFILRRIVCDVPPNALNKVFMNLGREIKKFSDYKENYSN
                     ILRYIFINKKSSQRFPTDSEFSNAFETKDIYNTKSKNKLYILERLENYNNKEIVDLEN
                     LINNNMLNIEHIMPQTLTNKWKESLGDNYKEIHDKYLHTIGNLTLTGYNSKLSNKTFE
                     EKKEMEDGFKDSRLYLNKYISSIDKWNEEEIKNRTKILLDRAVEIWKYPSVTYEPPSN
                     SENIFTLEDDDVNFTNESIVSFSILGESYKAKNWTEFYEQVALTLYDLDSVRFNSIVD
                     KTYKRDFIDNIKSRDAYKLRKGIKISENIYLETNLNTEAKLNIIRFLLNEFDIELNEV
                     IFSIK"
     gene            complement(44264..47599)
                     /locus_tag="MJE33_000140"
     CDS             complement(44264..47599)
                     /locus_tag="MJE33_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBY9646123.1"
                     /translation="MGNFDFLQKKKNFNSFNTACLEAEKSILVSPSTCATITRRALEL
                     AVKWLYANDSDLVLPYQDNLSTLIHNNSFIELIDYDMLPLLKYIVKLGNLSVHTSANI
                     ERGEAILSLNNLHQFVSWIDYCYSDEYTAEDFNEDLLLHGEEKRTRPDELKNLYERLS
                     SKDKKLEEVIKENEELRKSLTEKRKVNTANYDFKIDEVSEFETRKRYINIELKLAGWE
                     FGKDIWEEIEVQGMPNESGVGYVDYVLYGENGKPLAVVEAKRTSKDPKIGQQQAKLYA
                     DCLEKRYSQRPVIFLTNGFDMYIWDDYSDRKVYGFYKKSELQLMIDRRKSKKSLTSVT
                     INDEISNRYYQKEAIRAVCEALENKQRKTLLVCATGTGKTRIAISIVDVLSRHNWIKN
                     ILFLADRKALVKQAKKSFTKLLPNLALCNLLDSKDSPEDARMIFSTYPTMMNAIDDTK
                     SKDGKRLFTPGHFDLIIIDESHRSIYKKYKSIFDYFDSYLMGLTATPKDEIDKNTYSV
                     FDMENGVPTYAYEYNKAVEDGYLVDYTSIEFKTKIMEDGIKYDELSDEEKEEYENTFN
                     DDESIGDEIGNNAVNEWLFNSDTIDLVLNKLMTEGLKIEGQEKIGKTIIFAKNTKHAR
                     AIVERFNKLYPKYGGNFVKAVDYSINYVDSIIDDFSDKNKLPQIAVSVDMLDTGIDIP
                     EILNLVFFKKVRSKTKFWQMIGRGTRLCPDLLGVDMDKERFLIFDFCNNFEFFKFNPK
                     GFEGNKAETLTEKLFNIKVSMVKELQDIKYIEGEYAELRKELLEELITSVKALNEDSY
                     IVRMNLSYVHKYKNENVWSNIGAVAQNEIREYISPLITSYSDDELAKRFDVVMYNIQL
                     AYLQNNNASKGIRHVMATAEKLSKLGTIPQIQQQKYTIEKAMTEDFWEDSDIFEVEEV
                     RISLRELIKYLEKSSQKIYYTSFEDMIVAEDRNDSVYNANNLKNYKKKVEYYLNSHKD
                     ELAIFKLRNNKKITKQDVETLEEILLKQLGNCDDYKKEFGDTPVSQLVRRLVGLDREA
                     ANEAFSEFLNNKSFNTKQIHFVKLIVDYVVKNGFIEDNKVLMEDPFRTVGSIIDLFEN
                     HIEERNKLIKTINKIKENASEIG"
     gene            complement(47648..48076)
                     /locus_tag="MJE33_000141"
     CDS             complement(47648..48076)
                     /locus_tag="MJE33_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646124.1"
                     /translation="MVIRNKLSWNSIEEVNRDFGSCLYDLQNFKILYDKEEMPELWAR
                     YIKEGFKSYMVSFLELTKAMLYYKSIDLNIKSKNFYDYLLACEYHNLLPKNSSIVIET
                     LRKLRNDDSHGYDIPQFEDMYELFTENEEVFVSIRNSCKK"
     gene            complement(48066..48446)
                     /locus_tag="MJE33_000142"
     CDS             complement(48066..48446)
                     /locus_tag="MJE33_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBY9646125.1"
                     /translation="MRNIPNEIKNSIINVFPKENTLAIIIFGSYGTDKHTSESDIDIA
                     WIPIKKVPITELATKTEALRNILDIDVDLKIVTDNYTIALKKAILEGEVIYESKDFKD
                     YINKFYFENSDVIDILDWRDMYGN"
     gene            complement(48479..48787)
                     /locus_tag="MJE33_000143"
     CDS             complement(48479..48787)
                     /locus_tag="MJE33_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646126.1"
                     /translation="MNQIELDLYHNMVNIYKEADLQCNYKPTRFLQLINNKGALLAAK
                     ELINKEGATEGFTRLWECKRLDLSLEVLVLKEKYKELFTDEEREICINRLKDYGYKFN
                     "
     gene            complement(48820..50004)
                     /locus_tag="MJE33_000144"
     CDS             complement(48820..50004)
                     /locus_tag="MJE33_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBY9646127.1"
                     /translation="MVDEAKILDVVSISGENVKKFDGERSYLSTGNLDFNKISNLEIV
                     TYENKPSRANQTVNIGEVIFAKMKDTKKTLVINKTNKNIIVSTGFYVLKPSKEILPQY
                     LYHYLNSSYFLNQKNRLSKGATQSALNNEGLANIKIRMYNLKVQEKVVRVLDKAQELI
                     DKRKEQIEVLDELVKSRFIEMFGTPSKNEKNWEISEIGKYLDVLTDYHSNGSYETLRD
                     NVTLLDTKGYALMVRTTDLENNNFEKGVKYIDEHAYNYLEKSKVFGGEVIINKIGSAG
                     KVYLMPFLNKPVSLAMNQFMLRFNEDKVNHIFLYNLLLTSYMESKIKEKVRGAVTKTI
                     TKDAVRKINIIVPPIRLQNQFANFVKQVNSLKFEMETSLKELEDNFNSLMQKAFKGEL
                     FN"
     gene            complement(50008..51471)
                     /locus_tag="MJE33_000145"
     CDS             complement(50008..51471)
                     /locus_tag="MJE33_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HBY9646128.1"
                     /translation="MITGELRSKVDKIWETFWTGGLTNPLEVIEQFTYLLFIKGLDEV
                     ETKNEAEATLLGVSFERIFNDEHQHLRWSKFSNEGNSEKMYEIVQNEVFPFIKKLHGN
                     KDSAYAKYMGDAIFKIPTPLMLSKIVDGISNLELSKDRDTKGDLYEYLLSKVATAGTN
                     GQFRTPRHIIDMIVRLIKPTPEDIIVDPAAGSAGFLVSSQQYLRDNHSSLFLVQGLKE
                     HFNNGMFYGFDMDRTMLRIGAMNMMLHGVDNPNIEYKDSLSEVNTDKDKFTLVLANPP
                     FKGSLDYEAVSADLLKVTKTKKTELLFLALFLRVLKIGGRCACIVPDGVLFGSTGGHK
                     SIRKEIVDNHKLEAIISMPSGVFKPYAGVSTAIMIFTKTGTGGTDKVWFYDMKADGYS
                     LDDKRNEIKDSDIEDIIKRFDNLDGEVDRKRTEQSFFVDVEEIRENGYDLSINKYKEV
                     VYEEVVYDAPSVIMGRVKALEKEIVDGLEELERMLEV"
     gene            complement(51981..52850)
                     /gene="gatY"
                     /locus_tag="MJE33_000146"
     CDS             complement(51981..52850)
                     /gene="gatY"
                     /locus_tag="MJE33_000146"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBY9646129.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRVGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDDLDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIRDVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKNVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIETVKC"
     gene            complement(52870..54786)
                     /locus_tag="MJE33_000147"
     CDS             complement(52870..54786)
                     /locus_tag="MJE33_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HBY9646130.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKANELIQRCIDG
                     KVAIRKGNLSSSKIAEEGNLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPNSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSISGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNATKDEHKNESAESENVSNNISLYSEETVILDVKGKNKTDV
                     IDEMIGILDKSGVLLDKKKFKEEILKREEISSTGFGMGIAIPHAKTDAVKVPRVAVGV
                     SKEGFDFESEDGSLAHIIFMIAATDNGDNLHLKTLSQLSSKLMNEEFLNELLNSKTSR
                     EIVSKLNNEEIKSV"
     gene            complement(54789..55709)
                     /locus_tag="MJE33_000148"
     CDS             complement(54789..55709)
                     /locus_tag="MJE33_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HBY9646131.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFDVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLDKIQSLEDISYLSISGSLPPGIDDNYYEDIFKICKNKSIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGKSIYYASAQPVTVVSSACAGDSALAAFLSIWLENPEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            complement(55811..56563)
                     /locus_tag="MJE33_000149"
     CDS             complement(55811..56563)
                     /locus_tag="MJE33_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBY9646132.1"
                     /translation="MIIELNKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTINKSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
     gene            complement(56955..58400)
                     /locus_tag="MJE33_000150"
     CDS             complement(56955..58400)
                     /locus_tag="MJE33_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBY9646133.1"
                     /translation="MIKLGKFPKGFLWGGAVAANQLEGAWNEGGKGWCLADVHMYDKD
                     RDISKPYESDMTIERIKFAMKDKNGYYPKRSGIDFYHTYKEDLKLLKALGLKCFRFSI
                     NWARIFPNGDDLEPNEEGLKFYDNLINEVIDNGMEPIVTLLHYETPLNLVLKYKGWSN
                     RKVVDFFVRYAELILNRYKNKVKYWIVINQINLIHYESFNSVAFCVDQVDNVEEARYQ
                     AIHHQFIATALTKKLGKEINSDMMIGMMMADCTSYPEDCDPENVVFALKRNRLQYFFT
                     DVSFRGEYPVYIKRFFKENNINIVMEEGDDKILKENTMDFLALSYYYSTTVSASKNTM
                     NPTDNIKNPYLKANPWGWAIDPKGLYNTLSQYYDRYQKPIMIAENGFGMHDKFEDGKV
                     HDSYRIDYLGKHIEQIGEAIADGTEILAYCMWSPIDIVSCSSQEMEKRYGFIYVDIDN
                     NGNGSKERYKKDSFYWYENVIRTNGEELREK"
     gene            complement(58422..58775)
                     /locus_tag="MJE33_000151"
     CDS             complement(58422..58775)
                     /locus_tag="MJE33_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBY9646134.1"
                     /translation="MTIEEHIIDEELVEVAMQIILRAGEARTEIKHALNDLEKFDYRN
                     ADLKLAKAKGFMTEAHRAQTKIIQGEAGGEKRAHSLLFAHAQDTLMTIFSELNITMSL
                     VGIVKSIEYKVNNSK"
     gene            complement(58804..60051)
                     /locus_tag="MJE33_000152"
     CDS             complement(58804..60051)
                     /locus_tag="MJE33_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBY9646135.1"
                     /translation="MGRVFMNKFIDFLSEKFTPVVNNMTKNIWVQSVQSTIMKVLPMV
                     FVGSLVTIVSVLKNYISFLPDLSPINQYTFGLLGLFIAFLLPMEIMKNKKFESMSVVA
                     GLAGAGLMLMMIRPEITNEGAIFNFNRFGGEGMLVSLVAGLFSGLIMSLFGSFSFFGE
                     DSALPDFVSKWFDNMLPITVIMFVGWGLIYGLNFDMFEIIVRVFKPVSDIAQTLPGFV
                     LLNFLVVFIYSLGISGWVLSPITYSIQLGAIAANAAAVASGGVATNIYVYEVIQTGWI
                     TLGGLGATLPLVIMMCLSRVGRIKAIGRACIVPSIMNINEPVIFGAPVAWNPFLMLPM
                     WIGTVVISITTYLSMSMGFVTIPSKVFNLWYVPFGISTFMVNQDVRGLIVLAINIVLL
                     FMIWYPFFKAYEKEEIKLESQEV"
     gene            complement(60059..60376)
                     /locus_tag="MJE33_000153"
     CDS             complement(60059..60376)
                     /locus_tag="MJE33_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY9646136.1"
                     /translation="MKNIRVLLACGSGASTGFMATSMRKSAKKKGIEADIKARSESEI
                     DKYLSEIDCLLLGPHLDYMKEEIQQKANEYNVPVACISKTAYGRLDGAKALEEALELI
                     SNK"
     gene            60618..62531
                     /locus_tag="MJE33_000154"
     CDS             60618..62531
                     /locus_tag="MJE33_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY9646137.1"
                     /translation="MKTSKKELFLNFIKTQHDWIDSSTLANYLNVSTRSIRKYVNEIN
                     SNGEFILSSKKGYKVNLNNNCQTKVDSSENISPDNRLNLILKELIVNSNGINIFDLSE
                     ELFVSPATIEGDIVKANKFIGSYNLKIKQSKFLLKLIGNESAKRKLMSSIIFKETGSD
                     FLSLFDVQKIYQEYNLTKLKENIIYILKKYNLFINEYAINNILLHLMITIDRIKKNNY
                     IESVEVVNYIDNNNDISISRDISNFLESEYNITLTSAELYYLVFQLTNKTTVLNYHEM
                     DTKSLPNYIDEHFVKLTKKIIKNVYDLYLIDLSDEEFVVKFTLHVKNLISRAKNNQVL
                     RNQIPQKLKDSYPLIYDISVYICNQIQTLENVDIDEDEISYISLHVGSFFDRQKLLED
                     KVLCALITPNYYDLQFKIVRDLEKRFNESIEIIQIFSDTHNLDFDNKVDMVITTLPIN
                     NRCPIPFVYVNPYLNRKDYDNIQSKFTQIKDRKNILTVQNHLEMYFSESLFMKNIYLD
                     SAKDYIKFMGNILYENKYVKPNYIDDVLIREKMSSTAFNNNVAIPHSMKMDALKTGVC
                     LIVNDKPVKWGEEKVQIIAMIPINEKEKEKFNYIFESFIEILSEWNNVKELTKADNYS
                     SFMNRIAYLIQNI"
     gene            complement(62654..63808)
                     /locus_tag="MJE33_000155"
     CDS             complement(62654..63808)
                     /locus_tag="MJE33_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBY9646138.1"
                     /translation="MKILISIDSLKGSLSSIEAGNAIKKGILKVKEDAQVKILPLADG
                     GEGTVDALVQGMNGKKETIEVTGPIAKKVDATYGLLKNTSTAIIEIAQASGLTLVPTE
                     LRNPLYTTTYGVGEIIKEAINKGYRNFIVGIGGSATNDAGIGMLQALGFEFYDKNNKL
                     VGLGGKVLNEIRHIKIENRLKELDECKFKIACDVNNPLFGKNGAAYIYGSQKGATSEI
                     IEELDNGLRNFSKVVKNYLSKDVANVEGAGAAGGLGFAFLAFLNSKLESGIKIILEEI
                     KLEEELKDADFVITGEGRLDNQTAMGKAPIGVAKLAKKYEVKVIGLAGATTEDAVKCN
                     EEGIDAYFSIVNRAMTIEEAMDKATASENMTATTTQIFNLITSIQQSKNM"
     gene            complement(64087..66702)
                     /gene="mngB"
                     /locus_tag="MJE33_000156"
     CDS             complement(64087..66702)
                     /gene="mngB"
                     /locus_tag="MJE33_000156"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBY9646139.1"
                     /translation="MKKNVHIVPHMHWDREWYFSTEESRILLVNNMEEIMDMLENNHN
                     YPYYVMDGQTAILEDYLAVKPECKERIKKLVQEGRLIIGPWYTQTDEMVVGGESILRN
                     LLYGIKDCDEFGEYMKIGYLPDSFGQSAQMPQILNGFDIKYSMFWRGCSERKGTNKTE
                     FNWKSDDGSSVLVQILPLGYAIGKYLPMNEDELRTRMDKYLPVLDKGATTDHIILPNG
                     HDQMPIQKNIFEVIYKLKECYPERKFFLSRYENIFKELEKNEDIDTIKGEFLDGKYMR
                     VHRSIFSTRMDIKSANARIESKITNILEPLASIAYSLGFEYHHGLIELIWKEIMKNHA
                     HDSIGCCCSDKVHQEIMNRFFLAEEKVDQLITFYKRRIVDAMSCEMALDKLVAFNLMP
                     YDRNEIVSTQVITKMKAFEIFDKDNNKLDFEVVHKEVIDAGLIDRQIVHYGNYDSFMV
                     YTINFKDKIPAMGYKAYMIKEVEHMLEKAYEVCDMVDNDFYTIQVNENGTLKIFDKKL
                     NKTFENVLLMENGGDEGDEYDFSPLPNEKLIFNDNIVANSTIKKNRFNNEIKINYRLK
                     VPKDIQARKNNRADSFIDFDIVINVPNDKAIIDVVFNINNQARDCRVRTYIPTNIASK
                     FSVSDNQFGHIKRSVYDDAMEVWQQEEWSERPDSIYPMLTFVGLSDKEHGVAVLTNST
                     REFEIVGEKFDTIAITLFRSVGFLGKEEMARRPGRPSGIKLPTPDSQMIGDIKIDLAI
                     VTHEKSTLEANVANMAKEYLTKIQTYNKMPYNAMKLNQSEIEVDYSYSLLKETNKDLV
                     LSVVKKAEKEEALVVRFYNSKEETKSANIVFNKNIKTAKWINLNEKELESISIDNNSV
                     NVECLMNQVKTILVK"
     gene            complement(66846..68771)
                     /gene="mngA"
                     /locus_tag="MJE33_000157"
     CDS             complement(66846..68771)
                     /gene="mngA"
                     /locus_tag="MJE33_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HBY9646140.1"
                     /translation="MEFKKITNENLVFLKQKIKTKEDAIKFLARKLYYEGKLTSYEEY
                     LEAVMYRETLSPTGFERGLAIPHGKSKSVKEASFAAMTLETPIENWESVDPNNKVELI
                     FLIAVPENEGGNTHIQILSELVTRLSNEDYMNMLLNSKTKKELFNNLDIKEKKEQIKE
                     EIRIQNESKKIILAITACPAGIAHTYMSAEALIKAGKEIGVDVYVEKQGANGMVDPHT
                     PDIIKRADAIIYATDVAPKNTERFEHLPNIKTSVAAPLKRAKEIIYEALEVAQKQGKG
                     DYIEKSSDISYCEKSSWKKDVKSAILTGVSHIIPLIVAGGMILAFAVLASQAFGLQEL
                     YNQEGSWLWQLRKLSGGMLGTLMVPVLSAYMSYSLAEKPGLGPGFAAGLAADLIGSGF
                     LGGMLGGLLAGYIVKFLKKNIPAKGTLAGFVSFWVYPVVGSLVVGLLMLFVVGKPVAA
                     LNNGLINWLNGMTGVNAILLGAIIGAMVSFDLGGPINKAAYTFAIGAIASGNFIPYAI
                     FASVKMVSAFGVTIATVLGRDLFSESEKEIGKQTWILGLAGITEGAIPFMIEDPIRVI
                     PSLVAGSAITGSIVAFFNIGLQVPGAGIFSLALLEGSMSGFIIASIWFFAAVLGAIIS
                     AILLIITRKQKNQRKIH"
     gene            complement(68901..69626)
                     /locus_tag="MJE33_000158"
     CDS             complement(68901..69626)
                     /locus_tag="MJE33_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBY9646141.1"
                     /translation="MEFYERAKKYEYKFTDTEDMIIEYMRKNKEEIVKLSIQSLADKF
                     FTVPNTIVRLCKKMEYDGFSQLKNNLKEELKNEDKEPKNGFESDIKKTLELIDYEKIN
                     KVADIIQKSRSIIVYGIGQNIPLCEILVKEIRGGHDNITYYSQRHEVLHDIGKLGEQD
                     MFFALSLSGETKEVIEAAKTAVNKGCKVVALTHICENQLQQIADISLYCYSTIKQVNG
                     YNISDKVPIMMIMRAIIEQYWLR"
     gene            complement(69889..70659)
                     /locus_tag="MJE33_000159"
     CDS             complement(69889..70659)
                     /locus_tag="MJE33_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HBY9646142.1"
                     /translation="MKKLVVRADDIGYSEGVTLGIISSYKNGIVTSAGIMVNMPFSRQ
                     AIEMVKNDDVCFGLHVNLVIGEPCCKEHIKIKNLLDEGGKFVSSKIRRTQLLTGKEVF
                     NYEEVYEEVCAQIETYKLYMSKLPEYIDIHGIEEDVSISAMCDAAKEYGIPSCPYYLD
                     SCISMPSKYPQNDFYKLNKSFVKLFEEKYINLDKDITLMVTHPGFIDYSLIKSSSLIQ
                     ERVYEHAMLCDKEVKMWLKRNNIELVDFKDIFKSNNYI"
     gene            complement(70661..72088)
                     /locus_tag="MJE33_000160"
     CDS             complement(70661..72088)
                     /locus_tag="MJE33_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBY9646143.1"
                     /translation="MMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVPAISNISN
                     IVFSNVDYLFLLGAASAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNAGVFGGII
                     VGACIAWTYNKTYKVKVPAFLSFFAGEKCVITLSPVVALILSYIFSLLWIYPQAAMNQ
                     FGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTSTGEVIRGEIPRF
                     MAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKKKQEVKGAMTAGALTSFMATVT
                     EPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGVRLGSFNGASIIDYVLSFSYGDKAW
                     LVIPVGVVFFVLYYVTFKYIIIKDNVQTPGREVEIEIGDKVSEKEKNLKLSHGNFSYM
                     AKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLSNTSVQII
                     IGTDVVKVKDEMEMQLDELRKQADA"
     gene            72479..73201
                     /locus_tag="MJE33_000161"
     CDS             72479..73201
                     /locus_tag="MJE33_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY9646144.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNTEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKVGSEVYYLHRLRYIDNIPKILDINYFLCSIVKDLDVSIA
                     QGSIYKYIEECLGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            73398..75065
                     /locus_tag="MJE33_000162"
     CDS             73398..75065
                     /locus_tag="MJE33_000162"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBY9646145.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLYSLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMSDFEKLLKEAHKRDIKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDETTNQYYL
                     HLFEETQADLNWENEKVREECYKILEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGAKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNAY
                     YHMIKSGIDEKEALLILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSRSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKNQNLLVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRDIIENKMEIKPYESIMIYYK"
     gene            complement(75161..76480)
                     /locus_tag="MJE33_000163"
     CDS             complement(75161..76480)
                     /locus_tag="MJE33_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBY9646146.1"
                     /translation="MEKGIKLGFWSIVLLGINAIIGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGDFVGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQVAEPLIKNIIIGILVVGLGIINFMGMKQSKHLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSASSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLAGSATPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPKSIRKTNKNGSPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYAGKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYSKKYNDVEVK"
     gene            complement(76521..77267)
                     /locus_tag="MJE33_000164"
     CDS             complement(76521..77267)
                     /locus_tag="MJE33_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBY9646147.1"
                     /translation="MKKLAKKKYPIIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVM
                     CKAIPYIVPIVYDDEIIKEQVSNIDALILSGGQDVNPLIWKEEPHNKLGAISPKRDSF
                     DMKLLKHALDMKKPVLGICRGEQIINVTEGGSLYQDLSLIEGAYIKHNQQHLSNVPTH
                     TVQIKEGTKLYEILGEKEVLVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFV
                     IGIQWHPEMMTRDYDNMKKIFMAIVKEASK"
     gene            complement(77511..79520)
                     /locus_tag="MJE33_000165"
     CDS             complement(77511..79520)
                     /locus_tag="MJE33_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY9646148.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVMIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEGIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKIAITPGESSLVPKYIEEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGMAMVSDKGNIIICNNSLRDILDIQSNIIGKNIDELENENIKKIFSLSKAHDEV
                     IKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLSKKLREKGQVARYTFDD
                     IKTVSTKMERTKKLGMKIAESDYTILITGESGTGKELMAQSIHNASPRHSQPFIAINC
                     AAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLR
                     VLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKGDRFRADLYYRINVLPINIPPLR
                     ERKEDIEVMLKHFMKRKRAISQDVQDIINAYEWPGNIRELKNTAMYINIMSSEDVILI
                     NDLPHNLLNITEDFSKEISILKDRTSIEKVKLVMECLKNANNLNKNIGRNGIVNSINE
                     LGYNITEGEVRGILNMLKELDLIICESGRKGSELNRRGKNILNSI"
     gene            complement(79914..81347)
                     /locus_tag="MJE33_000166"
     CDS             complement(79914..81347)
                     /locus_tag="MJE33_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBY9646149.1"
                     /translation="MALRKDFLWGGATAANQCEGAWNLDGKGPSCSDMCTGGSQKVSK
                     RITRVIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYENVIDECLKYNIEPLITISHYEIPFAITEKYNGWVSRDVIDLYMNYCETIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFINQVDIPQLRFQGLHHQFV
                     ASAKAVKLAHEIDSNYQVGCMQIFATMYPYTCNPDDAVKTQRDSRVMNYFCGDVQVRG
                     EYPTYMNRYFGENNIEIKMEKGDLEILKEGCVDFYTFSYYMSTCVSSDSKEDNTSGNI
                     LGGVKNPYLKSSEWGWQIDPEGLRYALNEIYDRYRIPMMVVENGLGAYDKKESNGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMAKRYGFIYVDKYDD
                     GSGDLSRIKKKSFDWYKQVIESNGERL"
     gene            complement(81434..83350)
                     /locus_tag="MJE33_000167"
     CDS             complement(81434..83350)
                     /locus_tag="MJE33_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBY9646150.1"
                     /translation="MRKYEQLSKEIIANVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSAGQYQVVIGNHVPDVYEVVCDLAGLGGDTIDNSDSDAPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFTALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASTGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVILIVLLVAKLEKVFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGALTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGAFVPSFAQTAVVAAMYFKLKDKKIKELCIPAVISGICGVTEPAIYGITL
                     PRKTPFIYSCVGGAVAGAVMGIMNVKSYVMGGLGIFGIPNYINPTTGDMKGVYATIIA
                     IIVAIVVGFVLTLFFWKDDAIVEISKDDNGQGVIEKNKREVVYSPIKGEVKALEQIQD
                     AAFSTGILGKGVAITPKEGKVVAPFDGTVMTLFPTKHAIGIISDNGCELLIHIGLNTI
                     QLEGKYFKSFVNQGDKIKKGQTLITFDIESISKEGYCLETPVVVTNYSDYVDIVENTQ
                     KHVNNSQKLITVLA"
     gene            complement(83424..84284)
                     /locus_tag="MJE33_000168"
     CDS             complement(83424..84284)
                     /locus_tag="MJE33_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBY9646151.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGEHISKDKI
                     DKIFKLSDPNISDKFKELIADIPIRYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVSVKNVLLWDIKRFYKSEFKIGLEALDYIEKKFEIRLSEDEAGFIALHI
                     VNAQMDQSIKTIYEITQIIQEISNMVKYHYRIVFDEDSVYYHRFITHLKYFAERIVSN
                     NLHENNEDDLLNVIKVKYKNAYKFIEKLDEFIHKKYNYDLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
     gene            complement(84635..85822)
                     /locus_tag="MJE33_000169"
     CDS             complement(84635..85822)
                     /locus_tag="MJE33_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBY9646152.1"
                     /translation="MIESIKKRAYEIENGLGKEIEEVCNFIFNNPELGEEEYISSKYL
                     VEKMKEYGFDTVYPYCNMETAFRAELGDNDGPTIAFLAEYDALPGYGENKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKL
                     SYRYFENSFDNIINNKVLQKITKNNLIEAGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDISPMVYVHEKEFLNYANSEEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(85836..87227)
                     /locus_tag="MJE33_000170"
     CDS             complement(85836..87227)
                     /locus_tag="MJE33_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBY9646153.1"
                     /translation="MSSLKKKKFTMPQTLTIIFLLIVLMAILTWIVPSGNFERVDIDG
                     RSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEA
                     VIAHTVNKMKKFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTA
                     VATVFIGATAGFGAATTNPFSVGIAQALSQIVPGSGIEFRVVMFIIYMAISIGFVMMY
                     ANKVKKDPKKSLVHDISLNQELMVNSDTNIKEFTKREAMVIAIFTIGMAIMIYGVLRL
                     EWYITEIAMIFTAIGIISGIASGLKQDEIVNSFISGAGDLITAGLCIAFARGIVIIAE
                     NGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGD
                     LVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFVIPLMVVLTIL
                     VMAFLTIGLYLGF"
     gene            complement(87822..89522)
                     /locus_tag="MJE33_000171"
     CDS             complement(87822..89522)
                     /locus_tag="MJE33_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBY9646154.1"
                     /translation="MHYIAWHFNKNGGNNTMKDKLQVLSGAMMIPVILLVVAGVFIGL
                     GSAFANMENVSALGLSGLIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKK
                     EKGWGALGGIVLFIGMHTVISTLLAANGINSDTVTAEAFMASGLGEAEAYSRSSLYGS
                     FLGIFSYDCSIFGAIIAGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLG
                     TAMYFIWPPVGLALSKLGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVG
                     GTMEVGGAIYEGVNNIRLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLAD
                     NANKKKAISILIPAIVTAMLVGVTEPIEYTFLFVAPLLYFVAYVPLAGLTYVFTEMFN
                     VSIMGESFRNMFPNLLQPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSE
                     EVKLYSKKEYRERQSVDAGKTAYNNAVEEEVDDKDTKLVHSIIEGLGGSNNIKNVTNC
                     ATRLRVELNSIDGFYEDGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD
                     "
     gene            complement(89494..90897)
                     /locus_tag="MJE33_000172"
     CDS             complement(89494..90897)
                     /locus_tag="MJE33_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBY9646155.1"
                     /translation="MIVNKELIIDYLKSNQENIINDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIQIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGEKGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCNKIEAVFKTNDKKDLVESLAYYTEIEELEEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLEKIKKLNDKGVIIRDILNSYFTDDNHGKKLGIYKEDVDMGVSTICIMS
                     IFLEKNELNMKIDFRYPKGISWEFITNRINEIGKKENLIVDIYKDLKLLYVKPDSELI
                     NKLSNAYKQGFGKEAELFTKGAASYARVLKNGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
     gene            complement(91161..91652)
                     /locus_tag="MJE33_000173"
     CDS             complement(91161..91652)
                     /locus_tag="MJE33_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBY9646156.1"
                     /translation="MFNIFKKKENKIEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPSEGKLYSPVDGEIVNVFDTKHAIMIKSNDNTNILIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEVDLDFITSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            complement(91678..92490)
                     /locus_tag="MJE33_000174"
     CDS             complement(91678..92490)
                     /locus_tag="MJE33_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBY9646157.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENIKALKEVMEKGIKVALVTG
                     RPYNAMKYFTSVLGDDIYIISTNGTYFKLLGYEYKKVLSKETLKKIYTIGEKYNLNKH
                     FKGCKIVISNNEIGEEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVDSLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSEEVIC
                     IGDNENDISMIKFAGLGIAMGNATDEVKSMADFVTDTNVNDGVAKALRKILS"
     gene            complement(92503..93654)
                     /locus_tag="MJE33_000175"
     CDS             complement(92503..93654)
                     /locus_tag="MJE33_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBY9646158.1"
                     /translation="MINFYQPSRINFGQGTLSELPRIIGKYGKKCLLVTTPNVEPLDK
                     LYKRVTLNLIEKGIEVVHFDRVEPNPTVELINEGFELAKKESVDVVLAVGGGSSIDTA
                     KVLALTFGLEKIDWDYMFSTYTNPFKIYDKVSEKELPLIAVSTTSGTGSQVTQAAVIS
                     RGSEKNTIFHQNCFSKECIVDPELMLTLPERITASTAFDAFTHAFESYINPNANDFTE
                     IMSEKSMELVINYLPKVMEDKNNIEYRTKLAVADTLGGSSLANAGAAAPHPLSEIIGG
                     VTHIPHGEALAIVFPKFAEKFYKDNIEKFAKVARMLNQELEKVEDDEAAKTLGNEIES
                     FLKRVGLKTKMSEFNVTKEQLEQITSCPVLGFLPFGKKEDLVEILEKSY"
     gene            93924..94565
                     /locus_tag="MJE33_000176"
     CDS             93924..94565
                     /locus_tag="MJE33_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBY9646159.1"
                     /translation="MKYLIDSANLDEIQKCLKYGFKGITANPSMYLKEKVNFHEFIET
                     CANLNPEILTAEVIGNNLYELINSCDKILNINKNTIIKINFSEVGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICNLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTIDGANTFERDWSKVNK"
     gene            94591..96135
                     /locus_tag="MJE33_000177"
     CDS             94591..96135
                     /locus_tag="MJE33_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBY9646160.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEELKVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDIKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AIFELATVNKSYTELKEILFSKSNECKDTPSTKIFKELFNYDFEKLSQITLHTLQVEK
                     SNLSDEYYINFLIHIAICIKRISIKRYINLSEGFFRELLEHEYFEKSKILCANLSEAF
                     DVEFIDEEVCYILLHIEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGSILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
     gene            complement(96335..96961)
                     /locus_tag="MJE33_000178"
     CDS             complement(96335..96961)
                     /locus_tag="MJE33_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBY9646161.1"
                     /translation="MKKEMIKDYLLITISAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPLFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNINNACAQ"
     gene            complement(97096..97812)
                     /locus_tag="MJE33_000179"
     CDS             complement(97096..97812)
                     /locus_tag="MJE33_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentose-5-phosphate 3-epimerase"
                     /protein_id="HBY9646162.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTIGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAVDLVKRARKMIDEKNPKCELAIDGGLRSDNMEPLIECNPDVVILSSAIFKDKDGIT
                     AGVKNCRKAIDEAATKFGLE"
     gene            98238..99452
                     /locus_tag="MJE33_000180"
     CDS             98238..99452
                     /locus_tag="MJE33_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBY9646163.1"
                     /translation="MRNCIIAQSGGPTAVINASAVGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYKINSYDYDRVINILDEYKI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFIVETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIVSSSITTRVKTLELGVIQRCAMHLASDTDLKQAAEVGAYGIELSKKGYTGVMAII
                     NRTCNNPYKYEVSHTDISKIANKTKYFPKEWINEECNYITNEALNYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
     gene            100070..101398
                     /locus_tag="MJE33_000181"
     CDS             100070..101398
                     /locus_tag="MJE33_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646164.1"
                     /translation="MMKIAVITTEFLKEFVDNSIKKLNINAEIEIYIYRDFSHVGDLY
                     LEIEDRFDGFAVSGPIPKKAITKKAGTIKKPLVDFGTDLQSYYDMFLKLIFKYNTLDF
                     SRAYFDLTDWADNPKDISYYLSNGTFGDLMDSIDENASKMSLSDIAKLEKKIAKKHIN
                     LWNEGKIDFSTTRFSSIVPMLQNAGVNFHFIYPDIDLLKNTFNNLIKDIHLHRMKGNQ
                     PSVIYITVKDTNYDDSTLDQVYKILNSIKKDKLTDFIIEKNSNSFKIFTKCETIKSLT
                     KNFTACYIKESIENSTNIKVCVGYGIGNDIKQAMSSAISANKESKINHLNYSFLVNEN
                     YETIGPLLCDKLLTVSNKVTPHINTIAKSSKLSTLTIQKIISVTEILQRDEVTPKELS
                     SCLNVTVRTANRFLSSLLKSGNATILYEKQNSTKGRPERVYKLFISENKY"
     gene            complement(101526..102716)
                     /locus_tag="MJE33_000182"
     CDS             complement(101526..102716)
                     /locus_tag="MJE33_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBY9646165.1"
                     /translation="MKNFFKEAQSIEGEIIRWRRYLHENPEIGCNIPKTVTFVTSTLE
                     KMGYSPKLTSNNGIVAVLDSENGGKTLLLRADMDALPIMEETDLPFKSKNKDASHSCG
                     HDNHTAMLLGAAKILMDNKHLLKGRVKFVFEPDEETTRGAKSMIDDGILENPQVDAAM
                     AFHSMVGKFLNTGQVAYSRGPAMASADIFEITVEGKATHGASPEFGVDPINIMSHIQI
                     ALNTILSREKPQKEPVVLTIGMFNAGNVPNAIPNKASMSGTLRTFDQFVREKLKNRII
                     EISTGIAKSFGGNAKVEFKMGTPAVINDLDVGDEIVSYVKEVIGNENVIDFPSAMGSE
                     DFSEILLRVPGVFLWIGMGSISQGYNFGMHNPKAIFNEEGLVYGSALFAHCAVKWLEN
                     NFSR"
     gene            complement(102822..104201)
                     /locus_tag="MJE33_000183"
     CDS             complement(102822..104201)
                     /locus_tag="MJE33_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBY9646166.1"
                     /translation="MIGSISINEIDKKRFKLDDLSKKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEVGVGGLATAIRASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELGAY
                     GHGCGHNLLCTAHVGAVVGLKREMIENNLSGTIVFYACPGEEQLTGKGFMARGGAFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYTRALSREAVESTYERLVKVAKG
                     AAMMTETEVEVEFLGGCYNTLNNHVLANLVSECMNEIKQEPWTREELDFAAELDRQSP
                     QQYKAMISKYNLPEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYASKIMAMFGAKILNNPEIAKKAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            complement(104232..105440)
                     /locus_tag="MJE33_000184"
     CDS             complement(104232..105440)
                     /locus_tag="MJE33_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646167.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLI
                     GFWTNILPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALVGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNGDFLKDESKSNFKLPSMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIVPTIGILVVGSVFIILFTAIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
     gene            complement(106465..107919)
                     /locus_tag="MJE33_000185"
     CDS             complement(106465..107919)
                     /locus_tag="MJE33_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBY9646168.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYETPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPN
                     ELGIKYYEGLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLIDLYLKYSETVI
                     RRFDDKVKYWVTFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKVGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNITIEVTDEDKKAFKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPIGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDDY
                     RIEYLSKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKSNGEVLDFSTDIVVG"
     gene            complement(107939..109810)
                     /locus_tag="MJE33_000186"
     CDS             complement(107939..109810)
                     /locus_tag="MJE33_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBY9646169.1"
                     /translation="MKYKDLNENIIKLIGGKENIKSVAHCVTRLRFTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSIKSERKKGIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASMLALLNMSGILQSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQATTYANSFIPI
                     LLGVWAMGIIIEGLKKILPKALHYFLVPVLSLIITLSITLTVFGPIGMWIGDGLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPISLNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGI
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEVSNFTFMIISVVVSFVAATAASYLLGIP
                     SDEKEEVKGDNEKELELIINENNSRTISIGNVAKGQVIALGEVNDKAFSSGALGKGVG
                     VIPSESKSTVISPVDGTITIVFPTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESLV
                     TQNQRVKKGDALAVFESDKIIEEGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            complement(109800..110660)
                     /locus_tag="MJE33_000187"
     CDS             complement(109800..110660)
                     /locus_tag="MJE33_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBY9646170.1"
                     /translation="MKIIQILNNNVALVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RTYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKALNIKSSDYLSLTLLDHLEF
                     LLKRCEKNQFIKSPLVWNVKRFYPKHFEIGMHALKLIGDSKGLNLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVELDETSTNYMRFTTHLQYFIQRIMSD
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
BASE COUNT        36442 a        19653 c        11904 g        43175 t
ORIGIN      
        1 aactgcattg atttgaaaat aatattaata attaatagaa taaaaaattt aaattaaatt
       61 taagaaaatt tgttaagttg attgaatact tttttatgta tttaattaat tttataaatt
      121 caattatttt ctaaatttag gtatgcttta ctatataaaa atgatttttt atcttaatct
      181 tctgaatata agtgaatgct atcattttaa gtatcttata agcaatgtaa ctatatatct
      241 ctaataacta cttttttaac atgcttaaac tatttataag ctattatatt tatgtattaa
      301 cctaataact ttttcactaa aaaataacaa tctattcaaa gataaactat agtacttctt
      361 ctactttgtt tcaataaaat actaaataaa tttcgtacat ccacaatttt tcaggctaca
      421 ctataaatat acaatattta tcttagttat aagtttaaga atatttttag tcttattttg
      481 tcgaatatct ttttaataaa acttcataat tctaagcata tttttacact gtgttttcat
      541 gtacaaaaat aatgcttaca aataaacaac ataaataaaa cttaaataag atggattcta
      601 ttcaacatag tatccatctt attcattcaa aacctaaaag gattttttat aatattatat
      661 attttaaaat ctaagtaact cattatagta tcaatttcac ttaaagctta caatacactt
      721 attaatcttg ttttatatta aaaacatcta caataacaat ttaaatcttt aagtgatatc
      781 catctcacac ctaaaggagt aggctctccg cactattctg taattcttca atacaatcat
      841 tattattttt tatataattt aattatatat tttattttac caaaaccagc atacataaat
      901 aatacttaaa atagattaaa taaaggatac taaaacactt tattgaggta tatgacttga
      961 aatatatgaa ctaacttcat taggagaaat atctaaaata gttgcaaatt cttcatttaa
     1021 taacctttca gcttctttca ttatattatc atctgcttga tgtgtttttt taccaataga
     1081 ttttacatac tctctgttat tatctatact tcttactaat tttattaact cttcacattt
     1141 accacctctg agtatttttt taaattgctc acttctcaat ttttcattat ctatccatga
     1201 tgtatccata ttaggaatat catttattaa tgaaagcaca ttaattttag aaagtaactt
     1261 tctcattggt actttttcat tatctacagg aatttttatt actgtattat tagaataaat
     1321 aggactcaat acataatact ctttttttat tccatttgta aatcgttcat ttgttatatc
     1381 catgactttg cagactcctg tcataccata cattatataa tcatcaatct taaacaaaaa
     1441 catcaccatc cttaataaaa aaaatagttt tgacaactaa atccaccttg ccaaaactat
     1501 ctgctatata ttatatttta acacattttg agacttaaat gtaagtaaaa cttatattat
     1561 taaaattaaa aaatacatta accattgatt aatgtatttt ttagattttt ataacaagca
     1621 tatcaaaata aattaataca gcttatctcc tgtgaatacc tataaaattg gtgcttggct
     1681 attcattgag tacccatcat ttcttacatc atttatttct aatatttcat catttgtttt
     1741 aaccttagta agttttttaa ctgctccata aggttctttt aatgaaattt tatctcctct
     1801 tccagaaata acccataaaa ttttgtaccc tctaggtatt acttgtagtt tattttcacc
     1861 tttaccatca gtaaagtaga ctaacaaatt aatcttttta ttattagcat actcaaagac
     1921 tggagaaaat ctagtacctc cacctatcct aattctatct tttatatctt tttcagttct
     1981 cactttatat acacgtctaa tttcattatc acattctata attgtaattt catgattata
     2041 gtttttcact atactaagaa cttctttaat agcttgttta aactcttcat cactaatact
     2101 tccacttaca tcaagtgcaa ctgctatttc tgacttatga cttctaagtt ctcccctcaa
     2161 atctaaacga tttggttgtc ttctatttct tcttgttata gtcttctttt tattattttc
     2221 aactgttccc attattctat taagatataa attccatggc aattcaccct tactattttt
     2281 aagtgatgat atcatacttt ctaaataagc aggaacttta cctttttgag aactgttaat
     2341 aaatttttca gtaaattctc taagggtatt ttcatctatc tcattggatt catcccatat
     2401 atcatgagtt ctttcggggc tataatcaat ttctatatct ccatatttat cactatcatc
     2461 ttcctcacca tcttcctcac catcttcatc cacttccatc aaatctaatt cagtctgaat
     2521 cttttctaca taatattcaa aaggttcata tggttcaagt tttaaggaat attttaaatt
     2581 tacccactct aaggtagttg catatggtgg taaattgttt agaaatttat ttactactat
     2641 atccattgcc atattaattg ctaatgtact atactttcct tttaactcct ttgctctcat
     2701 caaatgcata gataatatat gatgaatttc atgtttaatt gtagtctcca tttgctttat
     2761 gtcaagcgtt aaaaagatta ttggattaaa atatataata tatttagctc ccttaaaatt
     2821 cacacctgta ggactactta tatcaaatct tatttctctt gacatctgaa acaaaaaata
     2881 tccataaaaa ttatcttctt cttccataag acttaaattc actttatcta caagactaaa
     2941 aaactcatac ttaaaatctt gtggtatatc tatatcaaat ttctcattag cacgatttgc
     3001 ctttagcata gaatgagtat ttataatttc acttgcttta ttataaagct cttttgcttg
     3061 tttatcaaaa taattttcca tacattatcc ccttattaaa ctataagatt caaaatatga
     3121 ttttacaaac tcttcatttt ctatagcttt tttatatact tcaatataac tgtttctaat
     3181 atccttcata atacctatca ttaaatctac aggatacatt tttaaaaatt caataaatct
     3241 attaatatag tggtttgatt catatttatc attcttaata tttaattcta agtcttttag
     3301 aatgttcata gctgatagat aaagtcttgt atgactctca ttttttactc tttctacaat
     3361 agactcatca atagattctc ctaaaaaaac atcttcatag gatcttaatg gttcagagtt
     3421 tgattcaata aaacttataa attcttctgc aataacttta cctacattac cctttacaac
     3481 atttaaaaat actgctctag gaatagaatt atttttttct ttataaactt tataaatttt
     3541 agatactctc tcataacttc taggagtggc tcttacatca tcttcattta ttttatgcag
     3601 atattctgga aaagtggaaa taaactcgat aacttttctt tcaataccct catctattgc
     3661 ccattttatc cattgtgtat ggtcactctc catatttaac caaacaaacc tattttcttg
     3721 ggctgcatcc atatctacca cttgataatc aaaatctgaa ccatatttac ttgatggatt
     3781 cattgctgct aaaatcttta catcatcatg cagtttatat ccattaattt ctctatttaa
     3841 aattaaattc ataagttctt gctgtactgt atgctcacaa cgatttatct catctataaa
     3901 taaaagaact gtcttacttt tagatatctc ttcatcaatt tctcttaatt tgttatgaac
     3961 agcatacact gttgttttct tttcagtctt atatccatta gaattaactc ctacataaga
     4021 ttctatagtt ggaagaccac ctatttcacc ctctttaagt aagtttccat caataacaat
     4081 taaactccaa tcattttctt tggcaagctt atttgctaaa gctgtttttc ctattccact
     4141 ttcacctact attaaaggta cttctccagt ggataatatt aagtcaacgc tttttaatgt
     4201 gtctataaaa ttcatttttc tctctcctac ataatcttta atttctcatc tacagctatt
     4261 ttttttgctg tcttacttcc atatttataa ataaacataa ttttatctat tggtatactt
     4321 tcgctatttc tatctataac actacaattc aaaaaatctc taatatactt ttcctcaaca
     4381 tcttctttgg ataatacttc ttttaataca ctctctaatt catctttaga tacttttttc
     4441 agaatgtact taatcactaa aatattcact tttaaaaatt ccaacatttt atttttatct
     4501 aaatcagtta taaagctaat agctgcttca ttatttttta tagctataag ttctgcatta
     4561 taactaggtg aatctatata tctcaaggca tcataactta ttctcactgc ctcctcttgt
     4621 acactttcat aaggttgttt tataaacttt atcgaatcat aattttttct tactgctaac
     4681 agttgtaatt cttcacttgg atttttagca tatttaattg cccatcctgc ttgatttata
     4741 gccatttcaa taactttatc agttgggttt ttgatataat ccagtatact ccatccctca
     4801 tttactgctt ttatcatcat atcttctgtt ggattatcta taaatttaat tgctctaata
     4861 ttattatcta ttgctagttc ttgcatctct ctggttggat tatttatata ttctagtaca
     4921 agcccatttt ctttaatagc taacaatttc atttcatcag ttggattatc tattgtagct
     4981 ataatattgg gattattttt tatcatttca ataactctca atttttccat aatgtgcctt
     5041 tccttctata attattttta tatctatatt aattataata atctattcct atttatataa
     5101 ttgtttctta ttataattaa tatacttttt aatcaaattt aaacagaata attataccaa
     5161 tatataaaaa aatttctaaa aaacaataaa attaagttat aaacaccaat atatttctat
     5221 tatttcaata agtatacaat tagattagaa tctaacgcta gtaataaaaa attactttcc
     5281 caaaatagat ataagcctca ttttatcgtt caaataatga aaatatattt gatttcatgc
     5341 aaaaagagtg cctaatgaac taaaaagttc attttgagac actcattttt ttatataaat
     5401 ttatatagtt tttaatcgtt cgcattcaac cactcagtta agacttttat atatttctca
     5461 ttttcttcaa cgaatggcat atgtcttgaa tactcaaata attcccactt actattaggt
     5521 attctatcat acatagtctt tgcaatatat ggtgaacata aatctattgc tccactagtt
     5581 actagacaag gctcttttat ttcatgtaat ctatctgtaa attcatatcc agaaagagtt
     5641 cctgtaggag aaaattcatt ttgtccccat cctacaatat atgcttcaga acctgatttt
     5701 ttaggtcttc tcaggcactc tggagaatct tctgtcactt cacctgcaca atacatttcc
     5761 ataaatcttt ctaatgcatc attatattct ttgctagagt aatctccagt atttactgca
     5821 tcaagtaatg ctttttggtc aacctcactc atatatgata ttcttctttt ttgttctttc
     5881 tcccaaagtt ttgcagaaga aagagtagat gaaaggatgt aagacttaat accttttggt
     5941 ttatattcaa tagcatacca aatagcctgc atacctcccc aagattgacc taacagatga
     6001 atttcatcaa gtcctagatg ttttcttagt tcaataagct cctctatcca agtatctgca
     6061 ttaaatagct caggatgccc ttctacaaat gaatttccac aaccaatttg gtcatacatt
     6121 attacttgtc taccactttc agctatctta tccaatacct caaaatagtt gtgtgtagaa
     6181 cctggacctc catgaagtaa tactaatggc ttcttccctt ccgtattttc tcctactata
     6241 cggtaatacg ttttaaaacc tttaaaaggc atatatcctt cagtaatttt catgtttatc
     6301 tcctccaatt tgtaattata aacaataatc aagttcatta acaatactat ttaaaacctc
     6361 ttatacatat ttgattatgt attttaatct ttatacctac ttttataaat agtataacat
     6421 aaaagttaaa aatctctata ctaaaccaat tataaaatta tattaactat ataattttat
     6481 tttttaataa tagtatatac atatttgatt attttcattt gctatttaaa atcaatttta
     6541 agttttcttc ttataataaa cttcctcttt gtggtctcat gttagtctct aaatttaaaa
     6601 ttttacaaaa aattttcata aaataaaatt atattgattg tatctaaaca aatagggcaa
     6661 taaaataatt aagtatagtt acttaccttt caagcaatat tagttttatg ttagaatttt
     6721 attatattga tttacaggag gtttgcattt gaataatatt aattcactta aagtaataaa
     6781 attatctctt ggatgttctc ttgctatatt tattgcttgg ctattaaaat tagaatactc
     6841 aatggttgct ggagttatag tactacttac agtaaaggac actaaaagag aaactcttaa
     6901 aggctccatt ggaaagattt atggttttct tttatgtact attttttcat atttatgttt
     6961 taatatttta ggatataatt tgacttcatt ttcaatattt atatttataa ttataccact
     7021 ttgttttttg ttaaatattc aagatgttat tgctatgtgt gtagtcatag catcccatta
     7081 ctaccttcaa ggagaaactt ctatcaaatg gattttaaat gaagctggaa tatttgcagt
     7141 aggaactgga ataggtgtct taataaatat gtatattcca acaaatatac ataaaatcca
     7201 tgaagttcaa aaaaagttac aggaagaagt gagtattgta cttatagaca ttgcagatat
     7261 tattgtaaat cctaaaaaag aaaatggata tagtagagat ttatatacat taaattcact
     7321 tattgacagc agtatttctg aaacttacga taatattaat aatactttat taagtgatac
     7381 tagattcttc ttagagcata tggatattat aaaaagccaa cgggatatct tagaaaattt
     7441 gtatagttat gtatctcaac taaattctac accaccacaa gctcatattc tatctgcttt
     7501 tatacataag ataggttata ctgaatttga agctgaaaca ggcaatcttc ttcttgaaga
     7561 attaaagaga ctaatgatta gtatgaaaaa ccagcctcta cctgttgaca gaacagagtt
     7621 tgaaaataga gctattttat tcctatgctt gactgagtta aaacagtttt tagttaatcg
     7681 taaacatgct cagatgctta gagataataa tttttataaa taaaaagggg agaaatctag
     7741 cagttgacct gactacaaaa tatcagatat aatattaaat gcgcccctag taagagggcg
     7801 caatgtttat aacttattct actgtcatga ttaaatcatt attcatatcg aacatacatt
     7861 atttgaccaa gtccaatatt agctttttca tcagtgattt ctattttaat agtcacatca
     7921 ttttttttac agtacttcgc taattctgca ccagaacgat ttttaccact aaaatctaaa
     7981 gaaaatggct tatcatattt aaaaggatat tcaacaggtt taccctctgg tgctaatgca
     8041 aacatcccat atggtgtgtt cataaatcct attacagcaa tcgtaagatt tgatgggtct
     8101 aaatttgatt cactacctgt aaatgtaatt gtatttactg atgaattaac tacactacat
     8161 ctaacacctt catacattac atgtttttca tttatatctt ctcctttttt tccaaaattt
     8221 gcaacagcct ttgccccttc ttgatgtggt aacatagtgt gcttcccagg tggtgctggt
     8281 ttctgtgtct gttctggttt taccgtctgt actattgcac caacttttgg atatgctgtc
     8341 ttatcaaatg gtaatgggtg gctaagactc ttgtatatga tatcctttcc tgctgattta
     8401 ttagcatcaa tatctacatg atacaaacgc ccatcaccaa aacggataag attccactgg
     8461 tggtcatttg atgtgctgtg tacataataa gattcaattc ccatagcatc acacaaagct
     8521 ttcatgctcc tagccattcc tgagcatgca ccttttttag taaccaatgt ataggcagta
     8581 tttgctccct ctactgcatt ccagtcaaca tatgttgtgt tatttttaat gtgattgtag
     8641 gcaactaaaa tcttagcatc tgtggtcata ttctttgtga ttttcttgtt cataaaatct
     8701 gctgttgctt gtgctactga gtttagttgg gcttgtgaca gctttggacc atatacgcta
     8761 ttggttccag tgacatagct ttttggtgtt tttgctgtta cctctttggc tgtcatacca
     8821 taaactgtca tactcatacc tgagattaaa actattgttg taatcaataa cttggtagct
     8881 aacttcctaa ttttcttcat acttaatatt ctcctttata aaacattgtt acttcctatt
     8941 ctctaccatt aataaaatta atttaggatt taaaaaggtg gattgtcaac atgtatattt
     9001 ctccattcat acattcgtga aatatttaaa tttcatggaa aatctaatag atattttcta
     9061 gataatttgt atacaataca actcgcaaat cagggtgttt gctgttagtt tgagaaaatg
     9121 atgatgatta atacattcgg aatctttgac ctataataaa taccattgtc ttcaatcatt
     9181 aataatggta agagtaaaga gaattatcaa taatacagtt aaaatcaact ttgtatacat
     9241 ttcatacatt ctctcccaac aacattatac ttcttataat ttgtatattc aatacgtttt
     9301 ctgggaaaat atagatatat ctataaaaat ataaaaaagg agttgaactt aaaatttgat
     9361 taaaaaaaat caattttaag acaactcatt tgtattataa aaatgctata tattattatt
     9421 aagtaagtca atattttaga tatttatcta tttcttattg acttacgctt atagtctaac
     9481 ttatttgttt agttgatata taacctagtt ttaagcatta ttaatattta aataatcaag
     9541 tatccttttt gtttcttgta gtttctgttc tgcctcattt aagttttctt gaattataga
     9601 aatatatatt atatctatca acgtaagcat tgctattctt gaagaaatag ctccttctct
     9661 aagtcttttt tctatatttg gtactactag ctttaaatca ctaatcttac taagatgatt
     9721 gtcactatat ttagttatac aaatgacctt acatcctttt aattttgcat tttctataga
     9781 ctttattact tcttttgatt ctccagagta agatattgct atagctatat cttctttatc
     9841 tacatttgaa gataccataa gttgtgtatg actatctaaa gaggtaaatg cctgtttatt
     9901 tatccttaaa agtttcatct gtaaatctag tgcaactaaa gccgaaccac ctactccaaa
     9961 tatatataca tttttagcct ttattatctg ttcaactgct tcttttatta tatctttttc
    10021 attcaacaac cttgtttggt tgatagtctc tatgtttttg tttgtaattt tgtttataac
    10081 actatctata ttacaatcat agttttcatc tacatatgtg tattcatttt ctttgcccat
    10141 caaatcttta gctatctcaa tttttaaatc cccaaaaccc gaatatccca tttttctaga
    10201 aaacctaact atacttgctg tacttgtttt tgagtttgac gccaattctg atacagaaag
    10261 cttacttata gcctctgtat tattaagtat gtaatcagct atttttttat ctacttccgt
    10321 taaatcttca tatatctgtt ttaattttaa aatgcaactc atactctacc acctctacct
    10381 atttactata atatttcttc ttagcaagta aacatgctcc taaatctgga gtcaacactg
    10441 gtctcactat accaaatctc gttttattca aatatttttt aagtggctct aatattaaat
    10501 ctcctgactt aaacactcca cctgaataag atacctttat ctttgtacct tgttcaaaat
    10561 gatgctctaa tcctattata agcctactta attcataagc tgcatcatca aatattttac
    10621 tagcagtatt atcaccttca ataactgctt ttaaacatat cgtagcaaac tttgctattt
    10681 ctattctatc tccttgtatt tcatcattta catattttat tatgtcataa tcattctcta
    10741 tattaagatg tctttttatt aaatcatata attttgtttt tgaacacctt ccatcagact
    10801 gcattgtgaa catttttaag gtagccattc ctatataata cgctgaacct tcatctccta
    10861 atacatagcc ccatcctcca catctgtgaa gattcccatc tttatctaaa gcctgtgcta
    10921 ttgaaccagt acctgcaatt atatttatac ctttttcccc atttaaagaa cctgctaatg
    10981 ctatttctac atcattacct agagtataat ttatatgact atatgcattt tttgttgcta
    11041 cttctaatgc atacaatact tcttttattt ttccatatcc agcaagtcca acaaatgcat
    11101 acgttatttc ttctacattt atcttagcat ctttacaaac ttcttccaac ccagttatta
    11161 ataatttcgt tagattatca aatcctattt gattataatg acaggttcct ttggttatgg
    11221 ttcctactat atttaattct tcatctacta atgtaaatgt tgttttcgtt cctccaccat
    11281 ctacacctaa aaaatacata ctatcctcct acaatatctt taataattct ttcaatactt
    11341 aatatctaaa aataaccttc caatgctgtt tttttaatat tgaaaggtta tcagtactat
    11401 tatttatttt acaatttttc tattgatttt aaagcttcac ttatataacc ttcttgtcta
    11461 tctaggatac tctttgcact atccttatct aaaccacttt ttatcataaa tatagccaat
    11521 ttaacgctat tatctgcttc ttctaagaat ttttctgcaa catcatattt tacatttgta
    11581 gctatcatta ttattctttt tgctctttca accaactttt catttgatgt cttaacatct
    11641 accatcaagt ttgagtatac ttttcccatc ttaaccatag aagctgttgt taacatatta
    11701 cataccattt tttgagctgt accacttttc attctagttg agcccattat tacttctgcc
    11761 cctactataa tgtttattgg taaatccgct tttttactca tttcactatt cggattcata
    11821 gttattgata gtactgcaca ccccaattcc ttagcatact ccattgcacc tataacataa
    11881 ggagtccttc ctgatgcagc tataccacat ataacatcct tagatgtaac tcctctatct
    11941 attatatctt gctttccaag ttctttatca tcttcagcac cttctattgc tttaaatatt
    12001 gcttgatttc caccagctat tatagcttga acaagctcaa aatccactcc gtaagttgga
    12061 ggacattctg tggcatctag tattcctatc ctaccactgg tacctgctcc tatatagaat
    12121 aatctacctc cctgtttcat tctatctact attatattaa cagcttttgc tatactctcc
    12181 ttttgttctt caacagcata ggctacagtt ttatcttcgt tatttattaa ttctattatg
    12241 cctaatgtat cttctttatc tatttgcaat gtattcttat ttctaccttc tgtaactaaa
    12301 ttttctaaag tttttattaa catatcattt cctcattttc ttaatattaa atttttaaac
    12361 acattttaat tccaactttc ataattatat ttttataata aattaatatt ttcataataa
    12421 attattttgt tgagggttta aaattaaact tttgccatgg tttaatataa tctaatataa
    12481 ataattcttc ttttcttata tatcctacta cattcgattt accactgttt ttcatatcag
    12541 tcaaggctat ttgtaactcc cctgcataat gaccatattc actactttca attactatat
    12601 ctcctctttt tattatttta ggagtattaa atagttcaaa tttgtatcct ttatatttaa
    12661 cacgagattg agtagacctt atcagactgt cagatatgtc ccctctatta aaatgtattt
    12721 cttcaaagag tatcttcttc tcaacttctc gtatattttc ctctattgct acatcaaatg
    12781 taaccatatc ttttctcata gaacctaata cctttagctc ctcatatgtt ggataacaat
    12841 ttgatattat tatatcattt atattattca tagctattat atgctttact tgaatatcta
    12901 taggtagatt tctgtgcatc tcaattgttg gaagcccatc agttacaggc catggtccaa
    12961 atgtagtttc attttgactt gttacaaaaa ctgctgttct taatccatac ttattaaatc
    13021 tttctgtaca ttttataaag tgttctaaat ttaatccact atatctatgt ggataaaagt
    13081 tatgacatcc tatcaaattt tctttatttg gttggtagtc cattattgta tctatataat
    13141 tagtattatt actcatattg atttctattt taagattttg aggattaaaa gtcattaatg
    13201 cctcttcaga acctgtaaat cccatatcaa gccttattcc gtctgccttc gtctttttaa
    13261 aaaaattcaa atctttgtag cttatttcta attcaccaaa tactctggga tttacatcaa
    13321 gtattacctc aaaaccttta aacttagcgt attcatttat acttttaaat tttttaatta
    13381 tattttcttt agtatcttta actgataata aacatgaaaa tatacgtgta aatccatttt
    13441 cagcagcttt atctatataa tcatatattt ccttagatgt tgttttttct ggataaattg
    13501 atattcctaa tcttcccatc tgtgtcatct ccttatatcc tatatgtgtt taaatttatt
    13561 tcgaattaca cgaaactttt ttaaaaattt gatgaaaaaa agtttcatat atatttataa
    13621 aaaaagaaaa gtttaccttt tcttttttta taaacaatca acaaaatatc ttcgttactt
    13681 atttcataac tgttatactt tgaaatttat actaatctaa tatagttttt aatttccaat
    13741 atagtttcta attaataagc ttcttgctct tcaacttttt cttcatgaag caacctctta
    13801 tcgtacatct tgacaaatgg atagtaaatt attattgata tagctataca taataagtta
    13861 agaactgaag ctctccaatc tccaccagtg gctaaatatg cccctactgg tcctggtaat
    13921 gtccaaggag cagtaactac tactctggat actaaattag cactcatagc aaaccatgaa
    13981 actgttgcca ttactattgg tgccacaaca aatggtatta ttaatattgg gtttaatact
    14041 attggtgctc caaatattat tggttcattt atattgaata ttgctggtat tacagttgct
    14101 tttcctaaag actttccata agctgatttt gctctaaatg caagaagtaa agctaagcct
    14161 attgtagctc cagaacctcc aatccatata aaccattgaa aaaagggttc agcagcaata
    14221 cctggaactg cttgacccgc agcatatgaa gctgtattag catctaataa ttgtatccat
    14281 aaaggtcttg ccaatgaacc tattattgat acaccatgta ttccaaatga ccaaaaaaac
    14341 gttattaaaa atattagtat aattactgat ggtaatgtgt cagtggctga aactaatgga
    14401 gttactaatt tagctatgaa gccatgccaa tcaaacccta ggaaataagt aacaatagcc
    14461 attaaaatta ttattaatgc ggctggtgtc agagcttcaa aagaccttgt gactgatgct
    14521 ggaactgctt ctggcatggt tatcttgaat ttagatttgt cagtaaatct ataaatctca
    14581 acacctatta tagatgttac aatagcaaca aacattgaac ctccaccaag attagccatt
    14641 ggcatttgcc atccttgagg aattgaatta taccaagcta aaagttctgg actcttttca
    14701 actatcttta ataaatcagg ggtaagaccc tctattgcag taggaactat tgtcaaaagg
    14761 aatgtagctg tagctaataa acctcctgtc aatccatcta acttatatga ctttgccaaa
    14821 ctataaccta tgccaaatgt agaatatact gatataataa aaaatgtcat tctatatggt
    14881 agtaatattg taactgcatt ttcctttaga tactgagtta ccccccatga atctggtaat
    14941 ggaggaaatg ctaatatcaa gaaaaatgac ccaactatta taagtggtaa tgaagctacg
    15001 attccatctc ttacagctct taagtgacgt tgttctgcca atctggccat agaagttgat
    15061 aatttagtgt ctaaaaattc tgtaaattta tctagcatct tttctctcct taattaatat
    15121 ttatttttat tatcattaaa atttttataa aatcaacttt cttctaatct aacaacactg
    15181 caactactat ttactcaaca gttcttgaac ttgttttaat aactttggtc ctcctaatgg
    15241 actataagct tgtgcttgta ttaatgcaca tggtacacta acctcatcag caaaagcttt
    15301 taaatagtca aatctatgtc tgacttgtgg agctaccata gcaatatccc atccattttt
    15361 aacttcattc tcaaactctt gagtaccaac agccaaaaca tctatatcta ttccattttt
    15421 tgctccttct ttttgtaatg cattaactgc tatagcactt gacatccctg atgaacaaac
    15481 aaataatact ctcatattga ttccccctgt ttttttattt cttttcaatc attatagcat
    15541 gtatttttga ttacaggaat acgtttgtat atattttttc atattttaca ttttaaattg
    15601 taattttatt tcatgtattt atatcttgct ttaattgctt tatttatcta atcattcttt
    15661 tgtctaaaat tacacactac ttaaaatact catatctttt cttaaaaaag aaaaaagact
    15721 atcttattat taacataaaa tagtctcaat acttttttaa atatgtattc ctttatatat
    15781 ccagaagtaa cctattattg caaatgataa agcaaataaa atatacagtc taaaaaatcc
    15841 caaagaaaaa cctctcttag tttcaacaaa gtatcttgta tatgcaactg caaaagatgt
    15901 aattaatgaa tttcctataa caactccaaa tctattatct attccaaata tagataataa
    15961 caaaggaaaa actacagtac ttactagaga tgaaactatt aatattaatc cacttttaaa
    16021 acttatagtt ggaatattat aatctgttct ttgatttttc tccatttgtt tcttagtatt
    16081 tttattactt tttgtacttt ttgcattttt ataattatta tttttctttc ttttcttttt
    16141 tttattatta gacatgtcat ttacctccaa aaggtttagg tgttatattc cctaaaataa
    16201 ctttattttt agcccctgtt gcagatatta cattagaaaa agaattattt aatgtttcat
    16261 tgcccaagat tacaaatgcc aaagcctcct ttgcatcaga agaataccca tattcatctt
    16321 gagttaatac ttcaacctct aataataact cttttatata tcctattaaa gttagattat
    16381 gagctcctcc acctccaatt aataaagtat ctacattaac ctttggaagt atgaagtttt
    16441 tataattata atatatagct tttgccgtaa acatagttac tgttgctatc aaatcatatt
    16501 tatctacatg tttatatttg ttaagcatgt tatccaaata aacttgtcca aatacctctc
    16561 ttccagtgct tttaggtggc gcttgactaa gatacttatg actcatcaaa tcttttaaca
    16621 tttcttcatt aattttacct tttgatgcaa agtatccatt tttatcatac tttctattaa
    16681 ataatctttg acatactcca tctattatca tatttcctgg tcctgtatca aatgcaaata
    16741 catcatctat gttacatgat ttaggtatta tagtaatatt tcctattcca ccaatatttt
    16801 gaagtgctaa atttttattg ttatcactat ataataaaaa ttcactatat ggtactaatg
    16861 gagcaccttc tccacctgct gctatatcca ttactctaaa attagatatt acctttgtat
    16921 ttgtttcata tgctataact gctggttcac ctatttgaag cgttgaagat acaaaattat
    16981 tataacttct tggtatatga aagattgttt gaccatgaga tgcgataaaa tccaaatttg
    17041 ctatatgaaa gtttgcttta tgacacactg attttacagc tttactaaat aaatatccaa
    17101 gcttaaaatt taagctacag attaaatcaa cactcgattc ctcttctata cagcactttc
    17161 ttatttcgtc ttttatatct ttaggaattt ctagagttat aaattctatt tgcttaactt
    17221 tagtatttct accattccca ctaatttcag ctagtactgc atcaactcca tctaaggaag
    17281 ttcctgacat taaacctaca ctatacatag ttatcatcct tttaagttta tagttattcc
    17341 tccccaaatc aaggtattaa gtttaataat tttagaatta atcttatttc atctttaatt
    17401 acgataattt actcttatga aattaattga gttaatctta tacacgtttt ttaccttagc
    17461 taaatcttac attaatttat tttcagtgtc aatgtaatac tatgtaattt tttttcacaa
    17521 acaaaacata ttctctctcc aaaaaccata ttaaaatagt tactacaaat aaaaaacata
    17581 ccaggtacat atctgatatg tttttcatat ataataatta ttattttata atatagatag
    17641 aacatcattt aaggcactaa ttaaatcttc actattttca agaactaaat ttattcttat
    17701 tgtatatgtc gatttgtata tgatgataga ttttatagtc taataaccta actatctagg
    17761 cttaaataat ctgatatatc ataaaaatag atattaaaaa ttgtatatga accattttcc
    17821 gaacatacct caatctcatg ccctaaacta tctatcattt tctttacaaa atacatcccc
    17881 attccagtag acttatacaa ttgatttctt ccatttttcc ccgtaaatcc cttgtcaaaa
    17941 accctagata tatcttcctc atttatacct ataccaaaat cttttataga taataccact
    18001 ccattttcta acttttttga attaaattca atcttaccaa cttcctttga atactttata
    18061 gcattattaa taatttggtc taaaacatat gacatgcatt tagaatctgt gtaaacatca
    18121 ttttctaatt cattcaaact aacttctaca ttatttttta taagcaaaaa tgaattattt
    18181 tttatagaca tttttaccaa tttttctaaa ttaaatttat ttataaatac atcttcaaac
    18241 atagtgttgc ttctactact atacaaaata gagtttacta aaaaatttat ctttgcaacc
    18301 tgattcttaa tatccaaact atcttctatg ctacttaacc tatctgtaat tatacttaaa
    18361 gaagatattg gaatttttat ttcatgaacc catttagcta tgtactcatc caattctttt
    18421 aatgattgtt tataagtctc tatgtcacta ttatttttta tttttaagtt ttctataata
    18481 tcttttatta gattttcttc aaaagattgt ccatcaacta aataactgtc tatttcacca
    18541 ctattttcta tacaattata taaatttgtg taactatttc taaaatttat ataatctata
    18601 acaaaaaata ccattattat aaacagcact aaaaaatcta aatatattaa ctctgaatat
    18661 ttatttttaa aaatatctat agcaaataag tagatattta ttatactaac tacaactaaa
    18721 ggtagagcaa tatacttaga cctacctttc aaatatttta ctagcattgt attatatatc
    18781 ctatcccctt cttagttttt ataaaatcat caaaacctag ttctttaact ttacttctca
    18841 atctattcat atttacagta agtgcattgt ctgtcacaaa ctcatcatta tcccatagag
    18901 tcatcataag actttccctt gatacaaccc tattcttatt ttttaataat aatgttaaaa
    18961 tttttatttc gtttttagtc aactctatac ttttatcctt atacttatgt tttataatcc
    19021 ctttccccac atcaaacatc aaatcttcac aatacaaaat actattagaa tcactataat
    19081 tatagactct tcttattata ccatttattt tggtaaccaa tatttctata gaaaatggtt
    19141 tggtaatgta atcatctgct ccattattta tacccattat taaatccata tcagaatctc
    19201 ttgatgataa aaatattaat ggtacatttg aaatagctct tattttttcg caccagtaaa
    19261 atccattata taatggtaaa tttacatcta aaagtactac atctggtttt ttatctataa
    19321 attcatctaa tactacctca aaatcatcaa ttaattctac tttaaatccc catttttcaa
    19381 gtgctaatgc aatctctttt gataaatcaa tatcgtcctc tactaataaa attctataca
    19441 tttgtatatt ctccattatc ctaatatttc actatgctat gaattattaa tatcataaca
    19501 tattttatat gaatttatta cccaaattat ttagttaatc aacaaggaga ctcttattac
    19561 aagaatctcc tttctcttta ttaatatatc aactaatttt tatacactac actcttaaac
    19621 ttattaacag atactgctcc atatattatg aatattatca aacatatcac taaacttagt
    19681 atatatatac caaataaatc ggtcatcaat aaatctcctg ctaatctcaa tgcaaaataa
    19741 ctagatatag ttgcacttaa cattggcaaa gtataaaaaa tagctacttc tttaagtata
    19801 gctctattta tatctttttc tgtagtacct atttttaaca aaatcttata tttctcttta
    19861 tcttcatatg catcactata tattttcata tatattatac ttccagctac aattgcaaag
    19921 actaagaata aaaacacact acatatgcaa acttcatcca ttgactctca ccttgagctt
    19981 tatatccatt ttgaatatac acatttttta aacttaaatt cttttcaata ttgttgaaca
    20041 ttacttgact attttcttct tgaaatacct ttgcaccata aaaatacact tgttgaccct
    20101 tatctcttag tttaaaatat gtatcatcat ttaccactat aatactttga ttaagtcctg
    20161 tccctagtac tggaactttt acttctcccc ttgaaatatt atataactga ccatcaagtt
    20221 ttatcttggc ttcttttgta gctattgatg ctatcgtttg agggcttttt aactctatta
    20281 cattatttcc actaactaat ctttcaccat actcttttaa ttctttatca ttgccattca
    20341 ctttaagagt attacaaaaa tcactatatt tcattatagt atctgttgta tcataagctt
    20401 cttttatatc tgttttcttt aaaattgtta gttctggatt tactgagtac tttatcttat
    20461 tagtatcttt tattatattc tctatttgtt ttttattcac ttcatcttta tccattgaaa
    20521 ctatgctaat agtgtacaca tttctttgtt cttgggcatt cttatgtata cgcttcatcg
    20581 caactgaagc accaagagtt gatattgttg tagttataat tatagctatt atagcataag
    20641 ttttatagtt tttatttaat cggtagttta tattgtttat tgcaattata ttatttccat
    20701 taaataatat ttttttattt ttgattataa tatcaaatat aacaggcata actgatttga
    20761 ataatccata agtacctact attattatac ctataacagg tagtacacta gttatatctc
    20821 cagttcttat attggtagaa agatagtagc catatcctat tactataatt gatactatag
    20881 ctaatatata tatacctatt cctacctttg gtattttttc ttgtttttta gatatattga
    20941 ttagatttat tacactactt ctacatattg ttataaaacc ttttacagcc attattataa
    21001 atatagaaat aaagattaaa gtagcatata gtatagcttc tatactaagt ccattagata
    21061 cctttatatt aaatccagat aactttatta tcactacttg aaaaaatcta gatgttatta
    21121 tccctataat tattcctgtt atacaagaac ttattcctat aagcatattt tctataaaat
    21181 atatttttcc tatagtatat aaatctaatc ccataaaaga caatattcca atttctttag
    21241 acctattctt aaaaaatata tttgttgcat accatataaa gaaaaataaa aatactgata
    21301 gtatcattat tacagctact aacactgata gtgtaaattt tttattttca tcttgtaaca
    21361 catttatagc atcaccatat cttaataatt caaaattagt tagcacaact actgaaaaaa
    21421 ccatagatat aaaaaatgct agataagttc ttatgttact cttaaaatta ttataagcaa
    21481 ttccaagtat actcacttac atctcacctc ccatagaggc tagcatatcc ataatttttc
    21541 cataaaattc ttttctacta tttcctttgt ttaatttaca ctttatattt ccatcattta
    21601 tcatatatac ttcatttgaa taacttgctg ttagagggtc atgtgtaatc attataatag
    21661 tagcgttatt ttctctgttt attttttcta atgattctaa aagttcatat gctgatttag
    21721 agtctaaagc accagttggc tcatcagcaa atatgactgt tggatttgta attaatgacc
    21781 tagctgctgc gcctctttgt ttttgtcctc ctgataactg atatggatat ttatctaaat
    21841 gattttctaa tccaaatttt ttagctatag aaaatacctt agcttctatc tccttacttt
    21901 tcttttttcc taatgctaga ggaagtgcta tattgtccat tagtttcata ttgtctaata
    21961 gattatagtc ttgaaatata aaccctattt tttctcttct taattttgat agttcattat
    22021 tttttacttt tgatatatta attccatcta gtattatttc tcctgaagtt tgtttgtcaa
    22081 gtgtagacat taaatttagc aatgttgtct ttcctgcacc agatggaccc attattgata
    22141 taaaatcacc ttcattgact gtaagagata tattttttaa cacatttgtt ttaaatcctt
    22201 ttattccata atcttttgat atatttttta tttttaatat ttctttcata ataatagcct
    22261 ccagttgcta attttttaat atatatttat tttaaaggtt ttatataaat aaatccttaa
    22321 gtaaatatta cattttaact gttagcatta cacttttgta acatatggaa gactattata
    22381 attatagtag aaatatctta aaataaaaac ttgcttcact tttttgtata aacaaagaaa
    22441 agaagctcct aagaagcctc taatctatac aataattctt ctctataaaa aaccaaaata
    22501 cacattgatt gtggcaaata cagatactat aaacaagtga tattactact atatattaaa
    22561 acaattattc ctgaagataa ttcaataaga ctatcaagca ataagctaag catccaaaaa
    22621 gacttaatac cattaatgta taattatacc caatattaat gctcaaaaaa ccagcaacag
    22681 ttggaataat cagaaatgaa cctcccataa atatacttaa agctattaat tggctactac
    22741 ttctaagttt tttaggagaa acttcaaata ttaatctttt tgaagtaatt aaaattaaag
    22801 gagtagaaaa cacttgaaaa acacacagta taataatgat atttgaatca tttgttaaag
    22861 caaataatag aaactgaact gttatcataa tagcacttaa ttttaataag tttaaactgc
    22921 tatatttttt taataaataa gaaccagcca gatatgttgg tatctctaac aatgactgta
    22981 aagaccactt taaagatatc tgagaatttg tagcatttaa agctatcatt ttatctatta
    23041 cagtacagtt atttgctacc accattgagt ataataagaa taaaatacat actagtaaga
    23101 tatatttttt atcttttaat aattcaataa tatctttatt agttacattt attttcttat
    23161 taatcttttc aatatcaggt aataatagta tatttataat aagtaatact acaattaaac
    23221 caatcgctac agataatcca aaatatgaga atctaagaat caagtaagat gctaccatac
    23281 ttccaatagc ccatccaatc gacccaaaag tcttaataaa tgacaaataa ttattaatat
    23341 tatctccaca acctattatc catgtatcat ataaactaca gcaagtattt aataatcctc
    23401 ctgataaagc aacaagtaat aagtatatta taaaagttgc agattcagta gaaaacataa
    23461 taactgatgc aattccataa actgataaac taattaaaac aatttttttc attgtttgat
    23521 acttatcagc taatactcca aataataatt gaaacaatat attggttatt gcatagctcg
    23581 ataatataat tcctctttca aacgctgagt aacctaattt tgataagaaa ggtgtgaatt
    23641 ttgttgaagc aaaggcaaaa ataatatatg agaaaaaata gaatatgata aatttaaatt
    23701 taatcgcttt attaatcata atcaatcaaa attgataact ttaataccac tatttccttc
    23761 ctgatttaag tttgaaagat gatattttac cgtattagga ttctctacaa taactaaagg
    23821 aatatataaa gtattttcaa gtaacttatt atcaatttct actaactttt cccccatatt
    23881 aacttcttga ccttgtttaa cttctaattt aaagttttct ttttttgttg ttgcactatc
    23941 caatccaata tgaataagaa tttgaacatc attttgcaat ttaattccta tagcatggtt
    24001 agtttgagca ataaaactta ttactccttt agtcggtgat ttaagaatat tgtcctttgc
    24061 tatcatagca aatccatctc ccattacctt agttgaaaat actttatcag gaatttctcc
    24121 aagattaatt atttttccat caatgtatga attaattgta attgcttctt tttttctttt
    24181 aaagaacatt atagacactc ctctacataa ttaactttat tctaattatc attacaaata
    24241 acgatattaa gcatactgca attgaggaaa aaagagcctt atagctcttt ccccattgta
    24301 gttctttaat actgcttatg atttgtttaa ttccaacaaa tttataaata taggtaaaca
    24361 tgataacaat agttattgtg taaatttgga atatctgccc taataagact aaatagaaag
    24421 caatcatgtt ttcttttatc tctttattct tttttatatt gtaaataaca tatgcactgt
    24481 atactgcact aaagttagag attaaattta aacttgcatt aacatcattg tttaaaagtt
    24541 gtgaaaatga aatatttgta aataatgatg tcaattctaa atacactata tataaaacag
    24601 gtaatattag aaaacaaacc ataaccactg aaagaaatat attttcatct tttaatactg
    24661 ctgtgatatt ttttatttta tttgttatca tgtttttgtt ctccttattt aaaagttggc
    24721 cagaaatcct tattagccac aatcatttca tctaagattt cttttgctac ttttgcactt
    24781 ggaacagttt tactcatagt aaacgactgc cataattttt gataactttt ttctatccat
    24841 gcttctacag ctaatttttc agaagcaact tgttgttcca ttaaaccttt ttggaaagtt
    24901 ggaattttac ctatagacat aggttcaacc atatcttttc caacaaggca aggaatttca
    24961 accattgcat catctgcaaa attttcaata gctccattat taggaacaat cagtaaaaat
    25021 ctttcctgag tattaaatgc taatgcacaa gctaattcaa caataaattg agaatgctca
    25081 cttgcatcaa accatgtatc ccttgctgtg ccatttttta taattctttc acattcttcg
    25141 aatgtatcct tttctcttct atccataatt tcatttgctc ttgtataatt tggattagca
    25201 tgttcaacag tttcatcagc cattaaataa tattttaaat aagtattagg taatgtagtc
    25261 ggttctattt ccaataaatc ctttgctgct ctcatagttt ctaaccaact agattctttg
    25321 tgttgacctt gaggtgtaaa ttcctcatat ccttttttag cacaatattc ttgtaattta
    25381 ggcattaaat catttccttc tttgtcctta atacttgtcc accacccgaa atgattcaat
    25441 ccataatatc taacatcaaa gtctttacga gaatttaaac ctaaaactct agcaaatatt
    25501 tcctctaaac atactggcat atcacaaata ttaataattc ttgaatttgg tcttaataca
    25561 cgacatgctt ctgcaactat agctgctgga tttgaataat taagcatcca agcatttgga
    25621 gaatattttt ccatataatc aatcatttcg attattggac ctattgttct taatccatag
    25681 gcaattccac ctgccccaca agtttcttga ccaacaaccc catattttag tggaattttt
    25741 tcatcttgtt cacgtaaagc atataaacca acacgaattt gtgccattac aaaatcaata
    25801 tcagtaaatg cttcttctgg atttgttgta taactaaatt taatttctgg atttttctct
    25861 ctaacaatta ttgcacatgc ttcagcaact tttctttgtc tctcatcatt attatcatat
    25921 aatttaattg attcaatagg aaatttagca acactttcaa ttaacattgt tacaattcca
    25981 gcagtataag tactaccccc accagcaatt agtatcctct gtctttttct ttccatcttt
    26041 ccttctcctt tataataaat tttcaaattc ttctcttaca cttggtactg aaagcccaat
    26101 aataatctga atagcttttc cattatgcac taaaccatgt gcacctactg ccttaaaatc
    26161 ttcttcttta gaaataattg agtcatcttt caaggttaat ctcaatctcg tagcacagtt
    26221 tgtaacatct acaacatttt ctgctccacc aactaatttt atgtattcat atgcttgttg
    26281 agaggcttta ctcaatttat ttcctttttc atctttcgtt tctaaatatt catttttagt
    26341 atatagtttt gtttcttcac tatcttcttc tctaccagga gttttcatat caaacttctt
    26401 aattaagaaa gtaaatacaa agaaccagat aaatgaaaat ataatcccta caacaacttg
    26461 aacaacatat accttccagt gatacattcc aagaggaagc caatttaata caacccagtt
    26521 aataattcct ccaccaaaat caccaacaac tcctaaggca aacgaaatcg tttgcaagca
    26581 tgcatctaaa aatgcatgta ctaggaataa aagtggtgct acaaataaaa atgtaaattc
    26641 taacggctca gtaattccag ttaatactgc agtaagagta gcaggaatca ttaaacctaa
    26701 tacttttttc tttttttctg gctttgcagt tacatagaaa gcaccaaata cacctaatga
    26761 accaaacacc ttcgataatc cggataatga aaatccgatt ggatacattt cctttagagt
    26821 cttagctgaa gtttgaaaat cctgaatatg agtagcccaa tatactgatg taccacctgg
    26881 aacaacaaca ctatcataac aaattggtgc atatacaaaa tgatgtaatc cagttggtat
    26941 taaaatcttt tgtaagaatg cataacacca aactcctata tttccactat ttataaaaaa
    27001 cgtttgcaaa tgtctaataa cattttgaat atgaggccaa ataaaacaag ttaataatgc
    27061 aactggtatc ataacaaaga aacaaattgc aactacaagt gatgagcctc taaataatcc
    27121 aataaaatct ggcagttctt tatcataaaa tctattatga atataaatga caatacctga
    27181 tattaacaaa gcaccaacca taccagtatc taatgtttta ataccagcaa tgcttacaag
    27241 tccacttcca gcacttattt cagctgcgaa atcaactcca aagctttttc cccaaaaact
    27301 taacatagaa cctacaaaat aattaaaagt caaatacgtt gctaaagcct ccatacaagc
    27361 tcttgcttgt tgcttttttg caagtgctat tggtaaagaa atagcaaata ataatggcaa
    27421 ttgattaaat accatccaag ctccactaga tattacaccc caaaattggt accaaatatt
    27481 ttctggtgta gcaggtgaac ccataattga ctgattttga aatacaattg agaatccaac
    27541 cattatccca gcaaaagcaa ataatagtgt tggtgtaaac attgcccctc caaaacgctg
    27601 tattttcttc atcatataaa ttactcccct cctcatattt ttagtttatc tttggcctta
    27661 gatatctatt attttaacag gttaataaat atatacaatc tttagggtac attcggagct
    27721 tattcacagt caaataagtt tttgcacatt tttatgttca aattctcata aaaaaaaaga
    27781 atgactacaa tttgcaatca atcccttctg taatcattct atgcctttta taaatactat
    27841 atttaataaa taataactca atcaatataa aatataacat tgtcaattta taactagggt
    27901 gcatttgtaa aaatgagata ttagtcgctg aaatataaat tgattcatca cttaaatcag
    27961 tcaatgtatt atttttaaac tctgtaattg aaattatttt agttcctatt aattttaatc
    28021 ttctagcaat attaagtgta ttctcattct caccacttag ggatataata atcataacat
    28081 catttttact gacaatattg taaaaagaat catcaaatgt tcttccctca aaatcatata
    28141 ttatttcttg acatgataag aacattctct ttatctgttt tgcaaccaca ttttgaatat
    28201 cacctgaacc ataagcgtat attgttcttg attcataaag aagtttacac aatgcatcat
    28261 aatctttatt tttttgatct tcaacatact taattattga attacatgca atatcaaatg
    28321 actgattttc tatgacatga accttttctg attcccagct taataatact ttaaactcac
    28381 tgaaaccttt taaatctaat tttttaacaa atcttgtaat agttgcacga gacacattac
    28441 atttcatagc aagttcattt attgacatct tactacaatg ttttttattt gcagttatat
    28501 atttccatat taataaatct gtatcattca atttattata atttaaaaaa atcatttctt
    28561 ctaatctcat aaactattac cccctttaat taaaaatcgt cataaatatt ataccaatat
    28621 tcaacattaa attacagcgt attcttatat ttactattta gcccataaaa ttgactataa
    28681 aaaatactgc tataattgat gtttaaaaaa atgtagtttt aattttattc ttttatctaa
    28741 taattaatat tttcaagttc tagtgctttt cttatacaat tatcatcttt aaaatcagca
    28801 gtccttttac aatcttttac tttaaattct ggaatagttt taaattcctc atcacatatt
    28861 ccattcttat ttaaatacat acaacttgcc attctaacta ttagaccact attttttaaa
    28921 ttaaacaata cagggtctat acaccctcca tctccgccag tagtttcacc aactaaagtc
    28981 gcaaatttat tttctttaca aaacattgca aatgactctg atgatgaata tatttttcta
    29041 tcaacaagta agtatatatt acccttaaat tttgaatcat ctgtagattt tatagtatag
    29101 gaagtatctt caaaatcatt aaaatctttt ataatttctt taggtgcatt ttcctttaca
    29161 cttgctggta aattttctac aggatttaat tttatatttc ttgcatttgt atattttttt
    29221 actatttcat tattatttct ataaattcta taaccattta ttttgacatc attttttatg
    29281 agtttagata cgattccctg ccaataccta tcacttccac ctaaattacc tcttatatca
    29341 ataattaaag ctttatgttt ttctaaagtg ttaatataat ctccaattat ttttaaatca
    29401 ttatttatag agccattctt tgatgacatt gatggtaaat acatatatcc aatctgaccc
    29461 ttaataacat cctttaaaat taattctttt tttacaaaaa tatcatctgg catttttatt
    29521 ttaagattca tagagtcata tctatccaca acttttttat cattaagaaa atcataccaa
    29581 tcattattag agtatgattt cttaaaaagt tcatatcttt tcttattatc tattacttct
    29641 gtgtgtttat tatttaaatc agataatata gatgacagtt cttctataaa ttcttcatca
    29701 ttttcagtgt tttttattcg ttttgtatat tcatccttat ttgctaacca atcaatatta
    29761 ttaagcctct tatttacacc tagatatgga tatcctgtct ttacaacatt atacacataa
    29821 ttaaagtcat ccaccttttc ctcttcagac aactgattct tttgagaata agtagaatta
    29881 cctaataata ttataaatgc taaaataatt actaaaaata atactttctt tttcatgtaa
    29941 ttctcatcca ctttcagatt ttatatttta atacattgta tcattatttt attatcacct
    30001 aaacagttaa aagaaattct tgaagatata ttccactatt attctattta aaaattttta
    30061 tttgtatttt caaaattatt tcatgaaagt tataaaataa caattatatt caaatatcta
    30121 ttttactgct atatcctgaa agtaaattct tatcagtgat atactaaaaa agatatgcta
    30181 aaaaatttat ccattataat gacttaaatt tagatacttt actgattttt atttattagt
    30241 tgagaaagat attgataatt aaaaatattg ataactatag aaatgattat tttttataga
    30301 aattttaagg atataaacta attatactta agtctatatc cttaatttat catgattatg
    30361 agaattttta caaatactta tctttgtccg ttttagaata aaaacaagaa catatcctgt
    30421 tattttattc aaacatccaa gtttaattac tacacaatta cttattagaa attgtatcta
    30481 cttcatatat atatctgtta agtatagctt ctagcatttc ttgtcttcca gacttattct
    30541 ttataactgg catttgcagt gcataagatt ctaactcttt aaatccaacc ttaccttcta
    30601 ctatatcctt acctatacct tcactaaaac ttgcatatct ttctttagta aagttctcaa
    30661 atacctcatc ttctaataat ttatgagcta ttaataatcc ctttgcaaat gtatccattc
    30721 ctgcaatata agctaggaat aagtcttcta cctcaaaaga tgctcttctt actttagagt
    30781 caaagttaaa tcctcctggt gctattccac cttgctttaa cacttcatac atagcaagtg
    30841 ttgcatcata tatatttgtt ggaaattggt ctgtatccca acctaataat aggtctcctt
    30901 gatttgcatc tatactacct aaaacattgt ttattcttgc tatatttaat tcatgttgga
    30961 aagtatgtcc tgcaagtgtt gcatggtttg cttctatatt cattttgaag tatttatcca
    31021 gattatactt tcttaaaaat cctaaaacag tagcagtatc aaaatcgtat tgatgtttag
    31081 taggctcttt tggcttaggc tctattaaga attgtccagt gaatcctatt tccttagcat
    31141 aatctacagc catttgtaag aatcttgcaa agttatccat ttcaagttct gtattagtgt
    31201 taagtaaagt ttcataacct tctcttccac cccagaatac aaaattttct cctcctaagt
    31261 acttagttat ttccatagct tttttaacct gagctgctgc atatgcatat acatctgcat
    31321 tacatgttgt agatgcacca tgaacaaatc ttggatttcc aaacatattg gcagttcccc
    31381 ataataactt tatacctgtc ttttccatat taacttttat atactcaact atttcatcta
    31441 atatcttatt tgtctcttct aaatctcttc cttctggtgc tatatctctg tcatgaaaac
    31501 agaaatactc aattcccatc ttagacataa attcaaatcc tgcatcaact ttaatctttg
    31561 ctatctcaat tgggtctgtt atattattcc aaggtctctc tactgtccct actccaaatg
    31621 ggtcattacc ctctccacat aaagtatgcc aatagctcat tgcaaatctt aagtgttctt
    31681 tcatagtctt attacctacc acttgttcag gattgtagta tctaaatgct aattcattgt
    31741 cagattttac tccttcaaat tttatctgtc ctattccttt aaatatttca ctcatttcat
    31801 gtcctcctaa tattttacac atataatata tgtagtatgt tttcttaatt taaaactatt
    31861 ttctatcttt atttttcttt ttaagctaat ttaattattt atttttgcat taactacttc
    31921 atctaataac ttaaagtcat ctttcaaaga tttgtatatt cttttataca atctatgata
    31981 tatattgtat gtatctacat tttcacttat tggttctaat ttagttgaag tttttattat
    32041 agcatcacaa gctttttgta tattttcata tattccagct cctactaatg caagaattgc
    32101 aactccaagt gctggtcctt cacttgaatt tatagtatat acatcttgat taaacatatc
    32161 tgcttgcatc tgtctccata ctttactttt tccacctcca cctgaggctc ttacttcatt
    32221 aacgtctata ttaagattct ctattatatc catacagtct ttaagactat atgaaacacc
    32281 ttccattatt gaccttgcga tttctttttt agaatggatt ggagataatc caaagaacac
    32341 acctcttgca taagggtcaa gatgaggagt tctttctccc atcatatatg gtaagtaaag
    32401 caatccatta catcctgttg gtactttact tacttgatta tttataatct catatacatc
    32461 ttcacctaaa gaatttgaaa cttccacttc actttgacag aaattatctt taaaccattt
    32521 taatgacaag ccagctcctt gagtaactcc cataacatgc catgtatttg gtattgcatg
    32581 acagaaagta tgaactctac cttctttatc aatttttggc tcttttgtat aagcaaatac
    32641 tacacctgat gtacctatag tagatgaaac tactccatct ttaactattc catttcctat
    32701 agctcctgct gcttggtctc cagctcctcc taccactatt gttccttctt taagccctgt
    32761 caaacttgct acatctctag tcacataacc tgtaatttct tgtgattcat aaactttacc
    32821 taaaagattt ttatctatat ttaacttttc tagtacttca tcgctccaat ctctttttct
    32881 tatatcgagc aactgcatac cacttgcatc tgatacctct gtagcatatt cacctgtcaa
    32941 tttgtatctt atataatctt ttggaagaag tattttatta gtatttttat atatatctgg
    33001 ttcattgttt ctaacccata atatctttga tgctgtaaat cctgtaagtg ctggattagc
    33061 agttatttca atcagtcttt cttcacctac taaatcagtt atctcctcac actcttttac
    33121 tgttcgttgg tcacaccata ttatagagtt tctcaatgtt ttaccttcat tgtccagaat
    33181 aactagacca tgcatctgtc ctgataatcc aattcctttt atacattcac tgcttatatt
    33241 tgaactttga actacttctc ttatagattc gactacagct ttccaccaat catttgcatc
    33301 ctgctctgcc caacctgatt tctcttgtat aagaggatat tcatatgtac aactttttat
    33361 agtatttcct agtttatcaa ataaaactgt tttcgtacct gatgtaccaa tatccacacc
    33421 taatacatac tccaacttca tccctccttg aatgccactt cataactatc gttttcctat
    33481 cctaattttt ttatatttta ggtatatttt acttgcttta tttttgttag tatattaaac
    33541 taacttatga actaataata aaatatttaa ttatctttgt caatgcaaaa taacctttta
    33601 tttttgtgta aaaagtatta caatattatt ttaaggagga atatcaaatt ggttactgat
    33661 aaatacacta ttcgagaaat gaatgaaaga ttggtacttg aacaaattat aaaaaatggt
    33721 cctatttcta gggcaagtat agcttctacc ataggattga ataaagcaac tatatccgct
    33781 attaccaaaa aacttattga tgaatctcta gttcatgaaa tcggaattgg aaatagtact
    33841 catagtggcg gaagaaaacc catattatta gtatttaata aatgtgcagg gatttcttta
    33901 agtatggaca ttggatacga ctatatattt tcttctctat cttacttaga tggaactatt
    33961 ataaattcta agaaattaac agatatacaa gtaagtaaag acaatgttat acaattgata
    34021 gatgaaatta taaatagtta taacatatcc aaaatagaca ctccttataa ggtaattggt
    34081 cttactttgg ctatacatgg gattacttgt gaaaataaag tattgtttac tccatattac
    34141 aatcttaatg agattgactt atatagcatc cttagcaaaa agtatgactt tcctattcac
    34201 atagaaaatg aagcaaactt aacagcttta gctgagaata ctttttcaac tgtacataac
    34261 tcactactta gtctcagtat tcacagtgga tttggttcag gcattataat aaataataaa
    34321 ttatatagtg gcagaaatgg tatgagtgga gaaattggtc acactatcat catgcctaac
    34381 ggtaaactat gtccttgtgg aaatcgtggc tgtttagaac agtattgttc agaaaaaaag
    34441 gtatttgaac agctttcttc tttagaaaat attcctaaaa tagattctga tatagtaaag
    34501 caactatatt atgaagataa ccagaatgcc aaaaaagtta ttcatgagtt ttgtagttac
    34561 ttaacaatag ctataaataa tgctattaca acttatgctc ctgaaataat atatttaaat
    34621 agccaaataa ttagtgatat ccctgagata ttacagatta caaaagatat gttagttagc
    34681 tcttttaaca aaggaataaa tatagaaatt tcatcattag ggtctgaggc atctctatat
    34741 ggtggctctg ccgtaaatat caagagcttt ttgaatatac aaaatttaac tttaattaat
    34801 gaaatataag ggttatgact atatacagcc ataacccttt aaattataca ttctaaaatt
    34861 agatttatta tatagtttat gcaagattag ctatattttt taaaaattgt atatcaatta
    34921 aatattaaca taagtatttc gttttaaata ttaatataat cttcctactc atcatcagat
    34981 aataaacttg cattttgttc aaccatagaa atattatctt tacctatttt ctctaatttt
    35041 gctgccaatt cctttgtatc taaaaaagct gattcaataa ccatacctaa atttagccct
    35101 gaaataattt taattcgact gtcattattg gctactaata aagcagcatt gcatggagtt
    35161 ccacctagta aatctgccat tataattatt tctgaacttt ccttgtacac ttctaaagct
    35221 ttttttaatt tttctattgt tccttctatt ccatcatctt gtttcatgtt tacagtagga
    35281 taatctatag aatcccctat tatcattttt gcagaattta agacttcttc agccatattg
    35341 ccatgactca tcaaaataat actattcaat ttaatacacc tctttaaatc tataagatgt
    35401 ctacaagata agttttctta tcatcaggcg tcattatagt tgtaatttca attccttcat
    35461 ctaaaagtct tttaaatgct tctttctctt cttcagtaac actgatatta ggtcttagaa
    35521 ctgttgtatc ttttctactt gacatatttc ctacatttat attatgtatt ttaaatcctg
    35581 cctctacaaa tggaataagg tccacaggag acttaacaat taataataca ttttgtcctt
    35641 catactttcc tgcagtaata ttttttatag cagtctcttc tgtaattata gatgacgcta
    35701 tacctgcagg tgtagccatt cttaaaactt gtttttgaac agaattgtta gctactgcat
    35761 catttactac cattaatctt gtaacaccca atttattact ccacatagtt gcaacttgac
    35821 catggattaa acggtcatct atacgaatat ttttaatacc tttcatttcc atatctaata
    35881 tcatcctttc cttattttaa aatacctaat gcatataaaa ttattgaaac aaccattact
    35941 cctaaaatta agtatatagg ttttatattc tttttaagag cccaataaac aatcacaact
    36001 aaaattgcag gaactaaccc aggcataatt tggtctaata tttcttgacc tttcattgta
    36061 aaatcacctt gttgaaagtt caaagcaaca ttagctttta caactgaagg tattaatgca
    36121 cctacaacca ttaaacctaa aatagatgta ctctcagtta aaatctttaa acttttttca
    36181 attgaagata ttagcttagt acctgatgta taaccagcat taaatagtgg tatacttaat
    36241 aatttcaatg ctacactcac tacaatccac agtaagattc caaatggact tccatctatc
    36301 gccatattag ctgctatagc tccaaaaatt gtccacggaa taacgacaaa taaactatct
    36361 cctaaacctg ctactggtcc catcaagcct gttttaattg aagcaacagc ctcttcagtc
    36421 tcttcacttg ataactcctc cattgctaca ttaataccca aaatataagg ggcaacccaa
    36481 ggattagtgt taaaaaattg taaatgattt aatacagcac tttgtaactt ttctggatta
    36541 tctttgtata ctacttttaa aaaaggtagt actgcataac agtatgccaa ggcctgcatc
    36601 ttctcataat ttgaagaaac agaactagta aaaaaccagc gccataagac actttttttc
    36661 accttttcat ttgatttatt taatgatacc ttatcactca tcttcgtcca tacctcctac
    36721 tacagtatat tgttgttctt gattttccaa attctttttg taaattataa gtgcaattgc
    36781 tccaccaatt aaagctacac ctaataatgg tactttcata tatactgcta aaacaaaacc
    36841 aattactaag taactaaaat actccttagc aggaagatat ctaagtaata atcctatccc
    36901 tactgctgga agtagtcctc cagctactgt taaaccacct gttaaccatt caggtgtata
    36961 tttcaaaatg gtattaatta aagaaggtcc aaatattaca gatagagcaa ctggaatacc
    37021 tgttgtagct gctgttaata gtactcctaa catttgcata taggttatat ttttataatt
    37081 tcctttttta gctccatctt ctgctttatg aactagccat ataccaatag tatttctaag
    37141 aacatctaat tgtaccatca ataaagcaac tggaatacct atagagattc ctacagatgc
    37201 ttcttgctta gtcgtaatag ttataaatgt ggctactata cttgctgttt gataatcagg
    37261 tattgatgct ccaccaaagt tactaatacc taatgataat agctgtaatg tacctccaat
    37321 atataaccct gtatttatat ctcctaagat aagaccagta ataaaacctg ctgttactgg
    37381 ttgatatgtt ccaaacattg ttgaattttt ttcatagttt ataaaaaatc catatagtac
    37441 gattaataat aattgccata attgtggctc cattatgatt tcacctctcc caaaatattt
    37501 tttagttctt taaaattata actggaatct tgctttagga aaataggaat ttcttccagt
    37561 gggcattcaa cttcataagt tttcccacct tcataactaa ctccactaaa tatattttcc
    37621 catgtatgtc cttttggtaa atatactttt ctacttctat ctttataatt cattattgga
    37681 gctactaaaa tttcatctcc aagcatatat gtattatcca cctcccaagt tttatcatct
    37741 tcaggaaatt cataaaataa agttctcata actggtgttc catcttcatg cgcttctttc
    37801 atcagttttt ttaaataagt ttttaagctc tctctaattt ttatgaattt tgtaagaatt
    37861 acttcaactt cttcaccata agaccatatt tcattaggag ctccagttac cattgagcct
    37921 ccacctttat ttgataatgg tttactatga ggcaatctat ctccatgcat tctcaaaatt
    37981 ggtgaaaaag tagcatattg gaaccatctt accataagtt ctctaaattc tgggtcttta
    38041 ggatttcctc catggaaacc tccaatatca gtagtccacc atggtatacc tgccagtccc
    38101 atatttaacc ctgtatttac ttgattatta aaagcctcaa aactagaatc aatgtcacca
    38161 gaccatacta aagctccata cttttgagct cctgcccaag ctcctcttac aagagttaca
    38221 acagactcta tgtcattatg taaaccatca taaatcattt ttaaataatc aataggataa
    38281 atattaccac aacttaaaac atttcctttt ttatatctat agttatcaaa atcatacaca
    38341 gaatagccag gttcagctac atctaaccaa taatagtcaa tcccaagttg tttataattt
    38401 ttatctattc tatcccaaac atattctctt gcattttcat ttgtcatatc tacaaagact
    38461 gtatttcctt gaatttgcat tgtcattcta acaccacgat taacattgac taaatagcca
    38521 ttttctaaat aatcattgta gttttctgca tcactttgaa ctgtaggcca tacagatact
    38581 attggttcta ctgaatactc ttctttcaat tttttaacta attctttagg atttttccaa
    38641 taatctaagt caaatttata ttcaccttgc ttaggccaat gaaaatagtc aattgcaatt
    38701 gatgaaagct taattcctct tttagaatat tccttcacaa catctaaaac ttcttctgaa
    38761 gttctatatc ttagtttact ttgccatagt cctaaaaggt tttcaggcat cataggaaca
    38821 gttccagtaa cttgagaata atttttattt aactcttttg ggctttctcc acaagtaatc
    38881 caataatcta taaaatttgt agaaaacatc ttccattctg tcatattttt agcaaaagat
    38941 acttttccaa ttcccggatt attccataaa agtccataac ctagactaga aatataaaaa
    39001 ggaactgata tttgagaatt tctttgggct aactccaaaa cagtattctt taaatctaaa
    39061 aatttatgtt gatattgtcc cattccaaaa atcttttcag aaggctcaga ttcaaatcta
    39121 gtagttactt caaattctcc actatagctt ggtcgatatt ctcttgattt taactttaga
    39181 gtagagttaa agtctttggt aatctcaatt gtccctacat cttctccacc atcatcatgt
    39241 tttacagctc ttaatcgaat atattctttc aataatattt catttttatc attataaaaa
    39301 gtaattctat ctctatggtc taagacagct tttatctttc catttttaat gattgcactt
    39361 ccatcttcat ttttattaat tgtaatatca tctttattta acttaggttt ttcgttaaga
    39421 gcatagtttt catcaacaaa attttggtct acaaacgacc taatacgtaa agagtttttt
    39481 ccccatgctt ctattcgcaa tagttcattg tcaaatcttt tttcaatata gtcttcagat
    39541 atttttatca cggctacctc ctaaatcaat ggtataatat tctatctttg tgtagcttaa
    39601 gttaaataat gtcgtttagt atcgttattt ataaattagg ttttatatat aattcatttg
    39661 tacattaaac taacttacca ataatatact ttatttgact ttgtttgtca atcgctttcc
    39721 ggattttagt aaaattttct aatatgttgc ttgaaagtcg ttcttaatat cttttattta
    39781 tacttttaat atctgtgatg atacctatat acatctagtt tttttaaaac acatacagaa
    39841 ataaagtttg cctattatgt agataatatc ggacttgaaa atatgtatag tgaccttata
    39901 aatacagata ataatatatt agatattgtt caaaacaatg gattttataa ttataaataa
    39961 tttatgaaat tatttaataa aatatataat tgtataccaa gtcaaaaacg acaacaaata
    40021 aataaaacta aaaaagatgt tgtctcaaaa tatagataaa tttatttttc attgctaaaa
    40081 tgattaaaat atatttgatt taatatactt gatttaatat aaaaaaagag tgtctcataa
    40141 actaaaaagt tcacttttag acactccttt atatatatta tctacttctt aattcattta
    40201 ttacctttct acattttctt tcagctagtg gatacatctt tataagtact aattactcca
    40261 cttaaaaatg gttcctgtac tgtccatcag tcatattttt ctgccaaata taatggtgtt
    40321 tcataatgag acaataaata tttatatctc tacttatctt ctctaagctc gtcaaatcca
    40381 actataggta tctctaaatt atattcttta agtagctttt ctagctcttc tataaaaata
    40441 cctttcccat ccaccatttg tatatcattt attttatcca tcaattcatt gcaaccactt
    40501 aactcaccaa accaacgttt agaactacat gtcttattat aaccacaact tggactgcca
    40561 tctattgcaa tgatactttt tatatcataa tcatttttta aatattcatc aaactgcctt
    40621 acatgaggta ttaacatttg cctacattgt tctctaaaaa acaaattgtc aaactgttct
    40681 tttacatgtc cccacctatt tattccatac ataatcattt ctggacatgg tagttgaatt
    40741 attccatacc ctttatttat taaatctatt gctatatttt gtgctccctt aaattctgac
    40801 aatccctcta ctttagaatt acaatttaat atacaatgac atactactac aatctctcta
    40861 tttctcttca taataatctc cttagttatt cttaatttta atgatactac atattttgat
    40921 aattatatta tttatttgtt ctatatttcc aatattctat atagaaatat acaattagta
    40981 atcttttata atctatacta tgataaatgt atatattttt ttaggaggta ttataattgg
    41041 atactaaaaa agtcacattt tcttcaatgt gcatagttat taatattgtc ttaggtatgg
    41101 ttatatctat gataaatata ccattattat ttttagatac agttggtact gtacttgggg
    41161 ctgtcgtatt aggtcctttt tggggtgctc ttattggtgg atgtacaaac ttagttttag
    41221 gtgttatatc aggtcctaca aatatacctt ttgcccttgt aaatattgtt cttggtctta
    41281 ttgttggata tatttctaag aaaaaaggtt ttggttataa agaagctatt attacaggag
    41341 taattttatc tattgtttgt cctttaattg gtactcctat ttcagtttta ttgtttggtg
    41401 gtttaagtgg aagtggtgct gatttattgg ttggtttttt agttcagtct ggtcagaaga
    41461 tatttactgc tgcgttcttt ccaaggattc ttagtaatat agtagacaaa cctttgtctt
    41521 gtattatggt ggtatatttt ataagtaaaa tgcctaaagg ttttataaaa caatatagta
    41581 aaaatacttc tagttcatat taataaatca tttattatgt aaggtttatt actctttaaa
    41641 atgctatata ctcattttta tataacattt ttattaataa tttaattaga tagtatttgt
    41701 attactttaa ttaaacgtaa cctcttgata taaatttgag ctataaatct atatcaagag
    41761 gtgctatttt atttttaata taattaatat tctattacgc aaaaaatgca tatatttcat
    41821 tattttatgg aaaatattac ttcatttaac tctatatcaa actcattaag taaaaatcta
    41881 attatattta atttagcctc tgtatttaaa tttgtttcta aatatatatt ttctgatatt
    41941 ttaatacctt ttcttaattt atatgcatct ctagacttaa tattatctat aaaatctctt
    42001 ttataagttt tgtctacaat actgttaaac cttacagaat ccaaatcata taaagtaaga
    42061 gcaacttgtt cataaaattc tgtccaattt ttagccttat atgactctcc taatatacta
    42121 aatgatacta tactttcatt tgtaaaatta acatcatcat cctctaacgt aaatatattt
    42181 tcactgttac ttggaggttc atatgtaaca cttggatatt tccaaatttc tacagctcta
    42241 tctagtaata tcttagttct attttttatt tcttcttcat tccacttatc tatagatgaa
    42301 atatatttat ttagataaag tctactatct ttaaatccat cttccatttc tttcttttct
    42361 tcaaatgttt tattacttag ttttgaatta tatcctgtca aagttaaatt tccaattgta
    42421 tgtaaatatt tatcatgtat ctccttatag ttatctccta gtgattcctt ccatttattt
    42481 gttaatgtct gtggcattat atgctctata tttagcatat tattattgat taaattttct
    42541 aaatcaacaa tttctttatt attataattc tctaacctct caagtatata taacttattc
    42601 tttgacttag tattatatat atctttagtt tcaaaagcat ttgaaaattc actgtcagta
    42661 ggaaatcttt gagatgattt cttatttata aaaatatatc ttaatatatt agaatagttt
    42721 tctttataat cactaaattt tttaatttcc cttcctaagt tcataaatac tttattcagt
    42781 gcattaggag gtacatcaca tactattctt cttaatataa aacattctac taaattaagg
    42841 gattctacaa gtactttttt atttattata ccttcattat aatcatcaaa taattctaat
    42901 ataaacggat atgatacaaa tatttcaagt ttgtttatcc ttttcaaaac tttgtttaca
    42961 tcattatcat cagtacatga ataagatatc tctctataat agttagaaaa tttaagtaaa
    43021 tcttttaata aaatttctat atctatacca ttatctgata catacctttt aaaactaaaa
    43081 tatactttat ttttattcgg tatacttctc tcctttatag tcaaatagtc tcttataaaa
    43141 ctacttacgt tgctacttgt atttttttca attttatacc aataattatt ataaaattcc
    43201 tcttgtaact tagagcttct acccattaac acaaagtttc ttactctatc agcttcactt
    43261 aaatctaagc cagttgaatt tagactttca aagattaatt gagggtcatc ttcaccattt
    43321 tttaactcta tttctactat agttaatttc tgtatagcat caaacaaatc atctattgat
    43381 atattatctt cttttattct attataaaaa tataaataat ttgaaactat attagaattt
    43441 ctatctgtat tcatttcttc ttcaaatagt tcatagtacg cttttctatc ttctgctatt
    43501 ggttttaact ttatttttcc atctttatct cttgagtgct tgtttataag ataacactcc
    43561 ttaatctgtt cttctgttgt aataattcct tttgattccc caaactcttt tattatttga
    43621 cacatggcaa tcaataatat tgacacagta gtcactcttt gttgtccatc tattattaaa
    43681 tattcttttc ccatactata tatagataca actgacccta agaaatgagt tctaaatcca
    43741 gaatctatta tatcctctaa atcatcaaac aatcttttac attgttcttt cttccagtta
    43801 taatttcttt gatatacagg tactacaaaa cttttgtttg acccctctaa ataactaaga
    43861 aattcaattt ctcctgcttt catattaact ccccctataa gttaaataaa ttccattcat
    43921 aactccatgt atctatatat tatatcaaaa ttttgactca taaatgtata tgaaagcctt
    43981 attatatacc tttttatacc tgtttttatt ttattaaagt aaatacatga ttattctata
    44041 aaatatacaa atacttaatt ttaaaaatta ttatttaatc ttcttcatga gtaattcata
    44101 gttatttatc aattaaaaag agatattgca taattattca atacctctct ttgtatattt
    44161 acattatcca tatctagtta tctatttcaa cccaatatta gactacctac taataaattt
    44221 attctctaca tcattaacaa taattttgca ctaaataaca tcattagcct atctctgatg
    44281 cattttcttt aattttattt atagttttaa tcaatttatt tctctcttca atatgatttt
    44341 caaataaatc aattatactt ccaacagttc taaatgggtc ttccatcaac actttgttat
    44401 cctctataaa tccattcttg actacataat cgactataag tttaacaaaa tgtatttgct
    44461 tagtattaaa gctcttatta tttaaaaact ctgaaaaagc ttcatttgca gcttctctat
    44521 ccaatcccac tagtcttctc actaattggc ttactggagt atctccaaac tccttcttat
    44581 aatcatcaca attacctaac tgttttaaga gtatttcctc caacgtttca acatcttgct
    44641 ttgttatctt tttattattt cttaatttaa atattgctaa ttcatcttta tggctattta
    44701 aataatactc taccttcttt ttataattct taagattgtt tgcattatat acagaatcat
    44761 ttctatcttc tgctacaatc atatcttcaa aacttgtata atatatcttt tgactagact
    44821 tttcaagata cttaataagc tctcttaatg atatacgtac ttcttctacc tcaaatatat
    44881 ctgaatcttc ccaaaaatcc tctgtcattg ctttttcaat agtatatttt tgttgctgaa
    44941 tctgtggtat agtcccaagt tttgaaagtt tttctgctgt agccattaca tgccttatac
    45001 ctttacttgc attattattt tgtaagtatg caagttgtat attatacata actacatcaa
    45061 atcttttagc caattcatca tcactatatg aagttatgag tggtgatata tattctctta
    45121 tctcattttg tgctactgct ccaatattgc tccaaacatt ttcattttta tacttatgta
    45181 cataacttaa attcatccta actatataac tatcctcatt taaagctttt acagaagtta
    45241 taagttcttc taacaattct tttctaagtt ctgcatactc accttctata tatttaatat
    45301 cttgaagttc tttaaccata cttaccttta tattaaataa tttttcagta agtgtttctg
    45361 ctttatttcc ctcaaatcct ttaggattaa atttaaaaaa ttcaaaatta ttacaaaaat
    45421 caaatattaa gaatctctcc ttatccatat caacacctaa taaatctgga cataatctag
    45481 ttcctctacc tatcatttgc caaaacttag tcttacttct cactttcttg aaaaaaacaa
    45541 gatttagtat ttcaggtatg tcaattcctg tatcaagcat gtctactgaa actgctattt
    45601 gaggtaattt attcttatca gaaaaatcat ctattataga atctacataa tttatactat
    45661 agtcaactgc ttttacaaag ttcccaccat actttggata cagcttatta aatctttcaa
    45721 ctattgccct agcatgcttt gtattctttg caaaaattat agtcttacca atcttttctt
    45781 gtccttctat tttcaacccc tctgtcatta gcttatttaa aactaaatct attgtgtcag
    45841 aattaaatag ccactcattt actgcattat tccctatttc gtctccaata ctctcatcat
    45901 cattaaatgt attttcatat tcttccttct cttcatcaga aagctcatca tacttaattc
    45961 catcttccat aatcttagtc ttaaactcaa tactagtata atctactaaa tatccatcct
    46021 cgactgcctt attatactca taagcatatg taggtactcc attttccatg tcaaaaactg
    46081 aataagtatt tttatctatc tcatcctttg gtgtagcagt aagacccatt aagtaagaat
    46141 caaaataatc aaagattgac ttatactttt tataaatact tctatgcgat tcatctatta
    46201 taattaaatc aaaatgacca ggtgtaaaca atctctttcc atccttagac ttcgtatcat
    46261 caattgcatt catcattgtt ggatatgtag aaaatatcat cctagcatct tctgggctat
    46321 ctttgctatc caataaatta cataacgcca agttaggtaa tagcttagta aaactttttt
    46381 tagcttgctt taccaatgct tttctatctg ctagaaataa tatattttta atccaattat
    46441 gtcttgaaag tacatcaact atagatatag caattcttgt ttttcctgta ccagtagcac
    46501 ataccaaaag agttttgcgt tgcttatttt caagagcctc acatacagcc ctaatagctt
    46561 ctttttgata atatctattg gatatttcat catttattgt tacagaagtt aaagacttct
    46621 tactctttct tctatctatc ataagttgta attctgattt tttataaaat ccataaactt
    46681 tcctatctga atagtcatcc caaatataca tatcaaaacc attagtcaaa aatattaccg
    46741 gtctttgact atatctcttt tccaaacaat ctgcatataa tttagcttgt tgttgaccaa
    46801 ttttcgggtc tttactcgtt cttttcgcct caactactgc taatggctta ccattttcac
    46861 cataaagaac ataatctaca tatcctacac cactttcatt aggcatacct tgtacttcta
    46921 tttcttccca tatatctttt ccaaattccc aacctgcaag ctttaattct atatttatat
    46981 atctttttct tgtctcaaat tcgcttactt cgtctatctt gaaatcatag tttgcagtat
    47041 taacttttct cttttcagtt agtgactttc ttaattcctc attttctttt attacttctt
    47101 caagcttttt atcttttgaa cttaatctct cataaagatt tttaagttcg tctggtctcg
    47161 tccttttctc ttccccatga agtaataaat cttcattaaa gtcttctgct gtatattcat
    47221 ctgaataaca atagtctatc caactaacaa attgatgcaa attgttaagt gaaagtattg
    47281 cttctcctct ctctatgttc gcactagtat gtacagataa attacctaat ttaactatat
    47341 attttaataa aggtaacata tcatagtcta taagttctat aaaactatta ttatgaatca
    47401 acgtactgag attatcttga taaggcaata ctaaatcact atcattagca tatagccatt
    47461 ttactgcaag ctctaaagct cttcttgtta tagttgcaca agtagatgga ctaactagta
    47521 tacttttttc tgcttctaag caagcagtat taaaactatt gaaatttttc ttcttttgta
    47581 aaaaatcaaa attacccaag cttttatccc ccttttgtcc cttagttaaa ataaatctct
    47641 ctaaaaacta tttcttacag ctgtttctta tactaacaaa tacttcttca ttttctgtaa
    47701 acaattcgta catatcttca aactgtggta tatcatatcc atgagaatca tcatttctca
    47761 attttctaag tgtttctata actatagaag aatttttagg taataaattg tgatactcac
    47821 aagctaaaag gtagtcataa aagtttttag attttatgtt taaatcaatt gatttataat
    47881 acaacattgc ttttgttaat tctagaaatg ataccatata gcttttaaag ccttctttta
    47941 tgtatctagc ccataactca ggcatttctt ccttatcata taagattttg aagttttgca
    48001 ggtcatacaa gcaagaacca aaatccctat ttacttcttc tattgagttc caagataatt
    48061 tgtttctaat taccatacat gtccctccaa tctaagatat ctattacatc actgttttca
    48121 aaatagaatt tatttatata atccttaaaa tctttagatt cataaataac ttcaccttct
    48181 aaaatagctt ttttaagtgc tattgtataa ttatctgtaa caatttttag gtctacatca
    48241 atgtctaata tatttcttag agcttctgtc ttagttgcaa gttctgtaat tggtactttt
    48301 ttgataggaa tccaagctat gtctatatca ctttctgatg tatgtttatc tgttccataa
    48361 ctaccaaata ttatgattgc tagagtattt tcttttggaa atacatttat tattgagttt
    48421 ttaatctcat ttggtatatt tctcatttaa tcacctcttg tttattaggt aatattactt
    48481 aattaaattt atatccataa tcttttaatc tatttataca tatttctctt tcttcatcag
    48541 taaacaactc tttatacttt tcttttaata ctaatacttc taatgataaa tctaatctct
    48601 tacactccca tagtcttgta aatccttcag tagctccttc tttattgatt aattctttag
    48661 cagctaataa ggctcctttg ttatttatca gttgtaaaaa tcttgttggc ttatagttac
    48721 attgtaaatc agcttcttta tagatattta ccatgttatg gtataaatct agctctattt
    48781 gattcataga ttttattccc ccattttaat aattgtattt tagttgaata actctccttt
    48841 aaaagctttt tgcattaatg agttaaagtt gtcttctagt tcttttaagc tagtttccat
    48901 ttcaaacttc aaagagttta cttgtttaac gaagttggca aattgatttt gaagtctaat
    48961 aggaggtaca ataatattta tttttctaac agcatcttta gttatcgttt ttgttacagc
    49021 tcctcttact ttctctttta ttttactttc catataggat gttagtaaaa ggttatataa
    49081 gaatatatga ttcactttat cttcattaaa tctaagcata aattgattca tagctaaaga
    49141 tactggttta tttaaaaatg gcattaaata aactttacct gcactaccta tcttatttat
    49201 tataacctca ccaccaaaca cctttgattt ttcaagataa ttatatgcat gttcatcgat
    49261 atacttaaca cctttttcaa agttattatt ttctaaatct gtagttctta ccattaaggc
    49321 ataacctttt gtatctaaaa gagttacatt atctcttaag gtttcataac taccatttga
    49381 atgataatca gttaaaacat ctaaatattt ccctatttct gaaatttccc agtttttttc
    49441 attctttgat ggagtaccaa acatctcaat aaatcttgat ttaactaatt catccaatac
    49501 ttctatttgc tcttttcttt tatctattaa ctcttgagcc ttgtctaaaa cacgcactac
    49561 cttttcttgc acttttaaat tatacatgcg tatttttata ttagctaatc cttcattatt
    49621 taaagctgat tgtgtagcac ctttacttaa cctatttttt tgatttagaa aataactact
    49681 atttaagtaa tggtataaat attgaggcaa tatttctttt gatggtttta aaacataaaa
    49741 tccagtcgat actattatat ttttattagt tttattaata actaatgttt tctttgtgtc
    49801 cttcattttt gcaaatataa cttctcctat atttacagtt tgatttgctc tagaaggttt
    49861 attttcataa gtaactattt ctagattgct tatcttgtta aaatctaagt ttccagtact
    49921 aagatatgag cgttctccat caaacttttt tacattttcc ccgcttatac taacaacatc
    49981 taaaattttt gcttcatcaa ccatactcta cacctctaac attctttcta gttcctctaa
    50041 tccatccact atctctttct ctaacgcctt aaccctaccc ataataacac taggagcatc
    50101 ataaactact tcttcataca ctacctcttt atacttatta atagataaat catacccatt
    50161 ctctcttatc tcttccacat caacaaaaaa actttgctct gttctttttc tatctacttc
    50221 accatcaaga ttatcaaatc tcttaataat atcctctata tcactatcct ttatctcatt
    50281 tctcttatca tccaaagaat aaccatcagc tttcatatca tagaaccata ccttatcagt
    50341 tccacctgtt cctgttttag taaatatcat tatagctgta gatactcctg cataaggctt
    50401 aaacactcca cttggcattg atattatagc ttccaattta tgattatcaa ctatttcttt
    50461 tcttatcgat ttatgtccac ctgttgaacc aaacaatact ccatctggca ctatacatgc
    50521 gcatcttcca ccaattttta aaactcttaa aaaaagtgcc aagaataata actctgtttt
    50581 ctttgtctta gtcactttta ataaatctgc tgacactgct tcataatcta aacttccctt
    50641 aaaaggagga tttgctaaca ccaatgtaaa cttatcttta tctgtattaa cctccgataa
    50701 agaatcctta tactctatat taggattgtc aacgccatgc aacatcatat tcatagcacc
    50761 tattctaagc atagttctat ccatatcaaa tccatagaac ataccattat taaaatgctc
    50821 cttcagccct tgcactaaga aaagacttga atgattatcc cttaagtatt gttgtgatga
    50881 tactaaaaaa cctgctgaac ctgctgccgg gtctactata atatcttctg gtgttggttt
    50941 aataagtctt actatcatat ctattatgtg tcttggagtc ctaaattgac cattagtacc
    51001 tgctgtagcc actttggata ataaatactc atacaaatct ccctttgtat ctctatcctt
    51061 acttaattct aaattactaa taccatcaac tattttagat aacataagag gcgtaggaat
    51121 tttaaatatt gcatctccca tgtactttgc ataagctgag tctttatttc catgtaattt
    51181 ttttatgaaa ggaaacactt cattttgtac tatttcatac attttttctg aatttccttc
    51241 attagaaaac ttactccatc ttaaatgttg atgttcatca ttaaatatcc tttcaaaaga
    51301 aactcccaaa agagttgctt ctgcttcatt ttttgtttct acttcatcca atcctttaat
    51361 aaataaaaga tatgtaaatt gttctataac ttctaatgga ttagttaatc caccagtcca
    51421 aaatgtctcc caaatcttgt ctactttgct ccttaattct cctgtaatca tatatgactt
    51481 tccctccagt gttcataaaa taatttaaat atatatttta gtcgtacgcc ttaatagtta
    51541 gtgtcttatg ttaaatccaa gtttttacta cacagttatt aaattatttt tcctatttac
    51601 atgttttagt taaaaaacta atgcaatact cttattttat gattattata tataccatac
    51661 aaaattattt tatcatatat ccatcataga agtcatctac ctggataatt cttaccatat
    51721 tataactttt taatattaat ttaaacttag aaagaacttt tttatttaat tatatcatct
    51781 tccttatttc aaatttctca attacaatat taatcaaaaa ataaaataag atgctgtctc
    51841 aaaatagata taatcttctt ttttattgtc agaatgatga aaatatattt aatttcatac
    51901 aaaaaagagt gcctcatgaa ctaaaaagtt cattttgaga caccccttaa tttaatctta
    51961 cttattcttt ccctttatta ttaacacttc actgtctcta ttgcttttcc tacacatcca
    52021 caaacttcca tcctactctt aacaaaatct ttaactgact ctaaaccttt tttaccatat
    52081 ttcttagggt caattacctc tgggtcagaa ttaagcacat tcttaactcc attgctataa
    52141 gcaatcctta attcagttgc ataattaacc ttgcaaatcc ctctttttat agattcctta
    52201 ataacttcat ctggtattcc agacccacca tgtaatacta atggaactga tacaacatct
    52261 ctaatttcag ataatctatc taaatcaagc tttggctctc ccttatataa tccatgtgcc
    52321 gtacctattg ctactgctaa agatgatacc cctgttctct ctacaaactc ttttgcttct
    52381 aatgggtctg tatatgctcc cccatcacca ccatctaaat cgtcttcctt acctccaact
    52441 ttacctaatt cagcttcaac aggtatatct gaaggcatac atgcatctac aacagattta
    52501 gatattgaaa tattatcttc ataactttca tgagaaccat caatcataat agaagtatat
    52561 ccaactctaa gtgcctgcat agctagtcca aagcttgaac catggtctaa gtgcattgcc
    52621 acaggaacac ttgccttctc tgatgctacc tttacattcg ctaaataata atctagtcct
    52681 gcatacttta ttgttgaagg tgttgtttgc aatattactg gtgatttaag ttcttcagca
    52741 gcctctatta ctgccataac catctccata ttttcaacat taaatgcacc tactgcataa
    52801 tgcccttctt gtgcatctaa taatatttgt ttagttgtca ctaatgccat aatttctcct
    52861 cctatatgtt tatactgatt taatttcttc gttatttaat tttgatacta tttctctact
    52921 tgttttgcta tttaataact catttaaaaa ttcttcattc attaattttg aagatagttg
    52981 agataatgtt tttaagtgta aattatcacc attatctgta gctgctatca taaatataat
    53041 atgtgccaag cttccatctt ctgattcaaa gtcaaaccct tcttttgata cacctactgc
    53101 tacacgtggt acctttactg catctgtttt tgcatgtggt atcgctatcc ccataccaaa
    53161 acctgtagat gatatttctt ctcttttaag gatttcttct ttaaacttct ttttatctaa
    53221 aagaacacca gatttatcaa gtattccaat catttcatca attacatctg ttttattttt
    53281 tcccttaaca tctaaaatta ctgtttcttc gctatataaa gagatattat tagatacgtt
    53341 ttccgattct gcactttcat ttttatgttc atcttttgtt gcatttgtat tttctatagt
    53401 atttttctca tttactactt taggtgtaac catcatataa agtacagcac ctactaatga
    53461 acctacaaat atacacccta cccataaaag tggcttattg actaatccta gaaccaaaaa
    53521 ccctccatgt ggagctggta cttctatttt cattatataa gttaaaattg atgatattga
    53581 tgatgcaatc ataagtactg gcaacacttt taatggattt tttgctgcaa aaggtattgc
    53641 cccctctgtt atatgagtac tacctagtat ataattgatt aatcctgctg ctctatcttc
    53701 ttctgtaaat ctatttttaa ataaagtaga tgctattgct attaccaatg gtggtgttat
    53761 acaagctgca gatacacctg ccataaataa ataatttccc tgtcctagaa gcatagttcc
    53821 agttacatat gctgctttat ttactggacc tcccatatca aatgctgaca tacatcctat
    53881 tactattccc aaaattatag gacttgaatt ttgtaacccc gatagaaagt tcatcatagc
    53941 attgttaagt gctgaacatg gcccaccaag taacaacatt gctacaccca caagacctac
    54001 gctgacaata ggaattataa aaatcgattt aagaccttct aattgtcgtg gtaattttct
    54061 taacaaatgt acaaatagtt taactaagta acctgctaaa aatcctgata tgattccacc
    54121 taaaaacccc gaacctgtat caaatgatat cattcctcca acaagtcctg ccaacatacc
    54181 agctcttccc gatatagatt gagctataaa tgctgccaat acaggaacca tcaagttcat
    54241 agaatatcca ccaacagatt ttaatgtagc tgctatttga ttatattgtt ctgaatttgg
    54301 gtcaaatgaa taaatccccc ataaaaatga tatagcagta agtactccac ctgctactac
    54361 taaaggtaac atgtgagata caccattcat taagtcttta tacaactttt gaccaaacga
    54421 taaatttcct tcttctgcaa ttttactgct tgataagttt ccttttctaa tagctacttt
    54481 tccatcaata catctttgta taagttcatt agctttatgt ataccttctt tgacaggtac
    54541 ttctattact ggctttccat tgaatctgtc catatcaaca tttttatcac aagcaactat
    54601 tattgcatct gccatttcga tatcttttgc tgttaactta ttttctacac ctatagctcc
    54661 attcgtttca acttttatgt cacaccctaa ttccctggct gaatttttta atgcttcttc
    54721 tgccataaaa gtatgagcta ttccagttgg acaacctgtt acccctagaa tcattttctt
    54781 tcccattttt ataccacctt tctgactctt atatttttta tataatcttc cacattctcc
    54841 aaattaccta taccattact ttcagccaca cttgctcctg ttgctgctga cctttttaat
    54901 gcttcttcaa tatgttctgg attttccaac catatactta aaaaagcagc taatgcagaa
    54961 tcaccagcac atgcagagct gactactgta actggttgag cactagcata ataaattgat
    55021 tttccattat aaaaataaga acctttttct cctaaagtta gcaatatatt ttgagcacct
    55081 tttaaatgta gtaatttaag tacatcctta atatcttctt catctcttac aataattcca
    55141 aatatatctt tgatttcttc atcatttggt tttatcaaat atggattata ctgtagtaac
    55201 tcctttaatt ttggtgaact tatatcgagt attgtcttta tacttttatt tttacaaatt
    55261 ttaaatatat cctcatagta attatcatca ataccaggag gtagacttcc acttattgat
    55321 aagtaactta tatcttctaa tgactgtatc ttatctagca tatctagttt ttgtttttca
    55381 tttacatatg acccagagtt tacaaattta aattcatcac ttccatcatt taaaaatata
    55441 tttatacgag ttgtatcttc tacccaagta ggcaatacgt caaatcctct cttttcgctt
    55501 tcttcaacta tatattttcc tgaaaatcct ccaaaaaatc ctaatatctt agattctata
    55561 ccataatgac ctagtacaaa agttacattt aatccctttc cattaggaga atatacagca
    55621 tttgatgtac gatttactaa ttttctcttt atcccattag tagatatatt catatctatt
    55681 gcaggattcg tagttaatgt atatatcatt gtcttccccc ttgctcgtgc tgtgtatgtt
    55741 ttcattataa taaaaaagtc tatgaaaatc ttttcacaaa tagaaaatcg attttcatag
    55801 acttttcaat ttaattttca gaattattat atctgaccat ataatctata attatcctag
    55861 atataatata caagggcaat ctggaagata cttcatattc agaaattgat atatgttcat
    55921 gtttgtatcc tacaatatat aaatccgaat actttataag ttttgcactt tcactacaac
    55981 agcaagttat aaccttagct ccaactaaat tggcattctg tgctgattct acaagctcta
    56041 tggtatttcc attcaatgaa aatataatta taagttcgtc tttttttatt gatttagatt
    56101 ttattttcat tatgttgggg tcatttataa aaacaacatt aaaatcaagt aattgcaatt
    56161 taagactaaa ctcttttgct acatattcag taagccctct tgccaaaata tatatcttac
    56221 tagatttatt tatagttttt attatattga ttagtgtagt taaagatatt tgttctataa
    56281 ctctctgtgc ttctataaag gatttattta ttatatcccc catattttgt atatcatcat
    56341 tttcatcttt tactgttaat ctatacctaa gttcattgaa tccatttaaa ccacattttc
    56401 taattgctct tgaaacggtt gctggagatg aatatgtttc atgagcaatc tctactatag
    56461 ataattctgg taatattgat tcattttcat ttataaattt aattatttca agttccgtct
    56521 tcgttagatt ttcagaatta cttttgttta gctcaattat catatgttat ctccccttat
    56581 ataagtttta tttgtctaaa tttatcgagt aatataatat attacctctt aactacttat
    56641 ttttatatac aattaagatt tagtcaatgt ctatatatgt aaatttataa tataataatt
    56701 ttttgcttaa agtttattat gttatcttat agcattttaa aataaaaaag aattgtacct
    56761 ctaccaaaat ttggatgaag tacaatagat attcctcata ctgatatgtt tatgattaaa
    56821 agtataaata taaatttcgc tatgaatcat ataattaata aatcatttta tttacaatta
    56881 taacccccct tataaaacta tattaataat atatacagta tttataaggg gggttgttta
    56941 tatttaaaaa ctatttactt ttctcttaat tcttctccat tagttcttat tacattttca
    57001 taccaataaa aactatcttt tttataacgt tctttacttc catttccatt gttatctata
    57061 tccacatata taaatccata tcttttttcc atttcttgag atgaacaact tactatatct
    57121 attggtgacc acatacaata agctaatatt tcggttccat cagctatagc ttcacctatt
    57181 tgttctatat gttttcctaa ataatctatt ctgtaactat catgaacttt tccatcttca
    57241 aatttatcat gcattccaaa tccattttca gctatcatta ttggcttttg gtatctatca
    57301 taatattgag ataaagtatt gtataatcct tttgggtcaa ttgcccaccc ccaaggattt
    57361 gcttttaaat atggattctt tatattgtca gttggattca tggtgttttt acttgctgat
    57421 actgttgtag aataatagta tgataaagct agaaaatcca tagtattttc ttttaagatt
    57481 ttatcatcac cttcctccat aactatattt atgttatttt ctttgaaaaa tctcttaata
    57541 tatactggat actctcccct aaaacttaca tcagtgaaaa aatattgtag cctatttctt
    57601 tttaaagcaa atactacatt ttcagggtca caatcttctg gataagatgt acaatctgcc
    57661 atcatcatcc ctatcatcat atctgaattt atttcttttc caagcttctt agtcaaagct
    57721 gtagctatga attgatgatg aatagcttga tatctagcct cttctacatt atctacttgg
    57781 tctacacaaa atgcaactga attaaatgat tcataatgta ttaaatttat ttgatttata
    57841 actatccaat atttaacttt atttttatat ctatttagta ttaattctgc atatctaaca
    57901 aagaaatcta ccacttttct attagaccat cctttatatt ttaatactaa attcaaagga
    57961 gtctcatagt gaagtagtgt tacaataggt tccataccat tatctattac ttcatttata
    58021 agattatcat aaaactttaa tccctcttca ttaggctcta aatcatctcc atttggaaat
    58081 attctagccc aatttattga aaatctaaaa cactttaacc ctagcgcttt caaaagcttt
    58141 aaatcttctt tgtatgtatg ataaaagtct attccactac gcttaggata gtacccattt
    58201 ttatctttca tagcaaactt tattctttct atagtcatat cagactcata aggtttagat
    58261 atatctctat ctttatcata catatgtaca tcagctaaac accaaccttt gccaccttca
    58321 ttccatgcac cttctaattg attagctgct acagctcctc cccataaaaa acctttagga
    58381 aacttaccca atttaatcac tccttatttt atttatttaa atcatttact attatttacc
    58441 ttatattcta tagactttac tattcctact aaagacatag ttatatttaa ctcgctgaat
    58501 atggtcataa gagtatcttg agcatgtgca aataacagcg aatgtgctct tttctctcca
    58561 ccagcttccc cttgtataat cttggtttga gctctatgtg cttctgtcat aaaccctttt
    58621 gctttagcta gttttaaatc agcatttcta taatcaaact tttccaaatc attaagagca
    58681 tgtttaattt ctgttctagc ctcacctgca cgaagtatta tttgcattgc cacctctact
    58741 aattcttcat ctattatatg ttcttcaatt gtcatattta taatttcctt tcaatttcta
    58801 aatttaaacc tcttggctct ctaattttat ctcttctttc tcataagctt taaaaaatgg
    58861 ataccatatc ataaatagca atactatatt tattgctaaa acaataagac ctctaacatc
    58921 ttgatttacc ataaaagtag atataccaaa aggaacatac cataaattaa acaccttact
    58981 tggaattgtc acaaatccca tactcattga taagtaagta gttattgata ttactactgt
    59041 tcctatccac ataggtaaca ttaaaaatgg gttccatgcc actggagctc caaatattac
    59101 tggttcattt atattcatta tagaagggac aatacatgct cttcctattg cttttattct
    59161 tccaactctg gataaacaca tcattataac taatggtaat gtagcaccca accctcctaa
    59221 agttatccaa cctgtttgaa taacttcgta aacatatata ttagttgcta ctcctcctga
    59281 tgctactgct gctgcattag ctgcaattgc accaagttga attgaataag ttataggaga
    59341 tagtacccac ccacttattc ctaaagaata tataaatact actaagaagt ttaagagtac
    59401 aaatccaggt aatgtctgtg ctatatctga tactggttta aatactctaa ctattatttc
    59461 aaacatgtca aagttgagtc cataaattaa tccccagcct acaaacatta tcactgttat
    59521 aggtaacata ttatcaaacc acttgcttac aaaatccgga agtgctgaat cttctccgaa
    59581 aaatgaaaaa gaaccaaata aagacattat taatccagag aataatcctg caactagcga
    59641 tacaagcatt ccttctcctc caaacctgtt gaagttaaat atagctcctt cattcgtaat
    59701 ctctggacga atcatcatta acattaatcc agcacctgca agaccagcta ctacactcat
    59761 actttcaaat ttcttattct tcattatttc cataggcaat aaaaatgcta taaataatcc
    59821 taaaagtcca aatgtatatt gatttattgg acttaaatca ggtaaaaagc ttatgtaatt
    59881 cttcaagaca gaaactatag ttactaatga gcctacaaat accattggca aaactttcat
    59941 tatagttgat tgaactgatt gtacccatat attcttagtc atattattta caacaggagt
    60001 aaatttttca cttaaaaaat ctataaattt attcataaac actctcccca taaatatttt
    60061 atttgtttga tattagttct agtgcttctt ccaaagcctt tgcaccatct agtcttccat
    60121 atgcagtttt agatatacat gctactggaa cgttatattc attagccttt tgttgaattt
    60181 cttccttcat ataatctaaa tgtggtccta gtaataaaca atctatttct gataaatact
    60241 tatctatctc tgactcactt ctcgctttta tatctgcttc tatacctttt ttctttgcac
    60301 tctttctcat actagttgcc ataaaacctg tacttgctcc tgaaccacac gccaaaagca
    60361 ctcttatatt tttcataata atacctccaa tatttttatc cattaattta tttcttaatg
    60421 aactttttat tttattatgt ttatattgta cttaccaaat aagttcattt cacataaata
    60481 tatttcctaa taataggaaa tcagttactc ttaattaaac atattaatat agtataatgt
    60541 atcttacaat acgtacattc aattatataa ttatcatatc atccagattt tattttaaaa
    60601 ctatgcgagg gaatctaatg aaaacatcta aaaaagaact atttttaaat ttcataaaaa
    60661 cacaacatga ttggatagat tcatctacat tggctaatta tcttaatgtc tcaacgcgtt
    60721 caattagaaa atatgtaaac gaaattaatt ccaatggtga atttatatta tcctctaaaa
    60781 aaggatataa agtaaatctc aataataatt gtcaaaccaa ggttgatagt tcagaaaata
    60841 tatcacctga taacaggtta aatttaattt taaaagaact tatagttaat agtaatggaa
    60901 ttaatatatt tgatttaagt gaagaattgt ttgttagccc agctactatt gaaggagata
    60961 tagtcaaagc taataaattt ataggaagtt ataatttaaa gataaagcaa tcgaaatttt
    61021 tattgaaact aattggaaat gagtctgcaa agcgcaaatt aatgagctct ataatattta
    61081 aagaaacggg aagtgatttt ttaagcttat ttgatgtaca aaaaatatat caagagtata
    61141 atttgactaa actaaaagaa aatataatat atatattaaa aaaatacaat ttgtttataa
    61201 atgagtatgc tataaacaac atacttcttc atttaatgat aactatagac agaataaaaa
    61261 agaataacta catagaatct gtcgaagtag taaactacat agataataat aatgatataa
    61321 gtatttcaag agatatatca aattttttag agtctgaata caatataact ttaactagtg
    61381 cagaattata ctacttagta tttcagttaa caaacaaaac cacagtctta aattatcatg
    61441 aaatggatac taagtcattg ccaaattata tagatgaaca ttttgttaaa ttaactaaaa
    61501 aaataattaa aaatgtatat gacttatatc ttatagattt atctgatgag gaatttgttg
    61561 taaaatttac tcttcatgta aaaaatttaa tcagtagagc caaaaataat caagttttaa
    61621 gaaatcaaat acctcaaaag ttaaaagatt cctacccttt gatatatgat atatctgttt
    61681 atatttgcaa tcagattcaa actttagaaa atgtagatat agacgaagat gaaatatctt
    61741 atatatctct tcatgtaggt tcattctttg atagacaaaa attattagaa gataaagttc
    61801 tatgtgcatt aattactcca aattactatg atttacaatt caaaatagta agagacctcg
    61861 agaagagatt taatgaatcc atagaaatta ttcagatttt ttcagatact cataatcttg
    61921 attttgacaa taaggtagac atggttatta ctactttacc aattaacaat agatgcccta
    61981 taccttttgt ttatgtaaat ccttacctaa atagaaagga ttatgataat attcaaagta
    62041 aatttactca aattaaagat agaaaaaata tacttactgt acaaaatcat ttagaaatgt
    62101 actttagtga atctttgttt atgaaaaata tatatttaga tagtgctaaa gattatataa
    62161 aatttatggg aaatattctg tacgaaaata aatatgtcaa acccaattat atagatgatg
    62221 ttcttataag agaaaaaatg tcttcaactg catttaataa caatgtagca attcctcata
    62281 gcatgaaaat ggatgcttta aaaactggag tatgtttaat tgttaacgat aaaccagtta
    62341 aatggggtga ggaaaaagtt cagattatag ctatgatacc tataaatgaa aaagaaaaag
    62401 aaaaattcaa ttacatattt gaaagcttta ttgaaatatt atctgagtgg aataatgtta
    62461 aagaacttac taaggcagat aactatagta gttttatgaa tagaatagct tatcttattc
    62521 aaaatatcta aatcaattaa tttatttcca atatatgttt cttaagttat taacataaaa
    62581 tagataagct gtcacactaa aaagtctaaa ttaatatgtg acagcttttt acctaatata
    62641 ccttgtttta atactacata ttttttgact gttgtatact agttataaga ttgaatattt
    62701 gtgttgttgt agctgtcata ttctcactag cagttgcttt atccattgct tcttctattg
    62761 tcatagctct atttactata gaaaaataag catctatacc ctcttcatta catttaactg
    62821 catcttctgt tgtggctcca gccaaaccta ttacttttac ctcatatttt tttgccagtt
    62881 ttgcaactcc tattggagcc ttgcccatag cagtttgatt gtccaatcta ccttcaccag
    62941 ttatgacaaa atctgcatct tttaactctt cctctaattt aatttcttct aatattattt
    63001 ttattccaga ttctaatttt gaatttaaaa atgctaaaaa agcaaaacct aatcctcctg
    63061 ctgctcctgc tccttctaca ttagctacat ctttacttaa gtaattttta actactttgg
    63121 aaaaatttct aagcccatta tctaactcct caattatttc tgatgttgca cctttttgag
    63181 acccgtatat gtatgcagct ccatttttcc caaacagcgg attatttaca tcacaggcta
    63241 ttttaaactt acattcatct aattccttta atctattttc tatttttata tgtcttattt
    63301 catttaaaac ttttccacct aatcctacta acttgttatt tttatcataa aactcaaacc
    63361 ctaaggcttg aagcatacct atcccagcat cattagtagc acttccacct atcccaacta
    63421 taaaatttct atagccttta tttattgctt cctttattat ttccccaaca ccataagttg
    63481 tagtgtataa aggatttcta agttcagtcg gaaccaatgt taatcctgat gcttgagcta
    63541 tttctattat tgctgtactt gtatttttta ataatccata tgttgcatcc acttttttag
    63601 ctattggtcc agtaacttct atagtctctt ttttaccatt catcccttga actaaagcat
    63661 ctactgtccc ttcaccacca tcagcaagtg gcaatatctt tacttgcgca tcttctttaa
    63721 ccttcaatat cccctttttt attgcatttc ctgcctctat tgaagataag cttcctttca
    63781 aagaatctat agaaattaat attttcatac ttatctactc ctttttatta ctccttattt
    63841 taaacatata attttataag cttaatttat cttttatttt atactctaca tctctctttg
    63901 tatacaactt ataccattaa tattctttta tcttataaaa agggagtgtc tcaaaataaa
    63961 tttcttagtt catgagacac tcttttgtat aaaatcaaat atatgtttat tatttcaaca
    64021 atgataagga ggattatctc tattttaaga taattccttt ttagcactat ctaatcattt
    64081 ttagcactat ttaaccagta tagttttaac ttggttcatt aagcactcta catttacaga
    64141 gttattatct attgatatag attctaattc tttttcattt aggttaatcc atttagctgt
    64201 tttaatattt ttgttaaata ctatgttagc tgattttgtt tcttctttag aattataaaa
    64261 tctaactact aatgcctctt ctttttcagc cttttttaca acacttaaca caaggtcctt
    64321 attagtttcc ttaagtaaac tatatgaata atctacttct atttctgatt gattcaattt
    64381 catagcatta tatggcattt tattataagt ttgtattttt gttaaatact ctttagccat
    64441 atttgcaaca tttgcttcta gtgtagattt ttcatgagta actattgcta agtctatttt
    64501 tatatctcct atcatttgag aatctggagt aggcaacttt attcctgatg gacgacctgg
    64561 gcgtcttgcc atttcttctt tacctaggaa tcctacactt ctaaataatg ttatagctat
    64621 tgtgtcaaat ttttccccta ctatttcaaa ctctcttgta ctgtttgtaa gaacagctac
    64681 tccatgctct ttgtcagaca atccaacgaa tgttagcatt ggatatatag aatctggtct
    64741 ttcactccat tcttcctgtt gccaaacttc catagcatca tcatacacac ttctctttat
    64801 atgtccaaac tgattatcag acacagaaaa ctttgaagct atatttgttg gtatataagt
    64861 tctaacccta caatctcttg cttggttatt tatgttgaat actacatcta ttatagcttt
    64921 atcatttgga acatttataa ctatatcaaa gtcaataaat gaatctgctc tattattttt
    64981 tctagcttgt atatcttttg gtacttttaa tctataattt atctttatct cattattaaa
    65041 tctatttttc tttatagtgg aatttgctac tatattatcg ttaaatatta atttctcatt
    65101 tggaagtggt gaaaaatcat actcatctcc ttcatctcct ccattttcca tcaataatac
    65161 attttcaaaa gttttattta attttttatc aaatatcttt aatgtcccgt tttcattaac
    65221 ttgaatagta taaaagtcat tatctaccat gtcacatact tcataagctt tttctaacat
    65281 atgttcaact tcttttatca tatacgcctt ataccccatt gcaggtattt tatctttaaa
    65341 gtttattgta tataccataa atgaatcata gtttccataa tgaacaattt gacggtctat
    65401 taatcctgca tctattactt ctttatgtac cacttcaaaa tccaacttat tattatcttt
    65461 atcaaatatt tcaaatgctt tcattttagt tataacttgg gtgctcacta tttcattcct
    65521 atcgtaaggc attaagttaa atgctactaa cttatctaaa gccatttcac aactcattgc
    65581 atcaactatt cttcttttgt aaaatgttat taattggtct actttttcct cagctaagaa
    65641 aaatctattc attatttcct ggtgaacttt atctgaacaa caacatccaa tgctgtcatg
    65701 agcatgattt ttcattattt ctttccaaat taactctatt aatccatgat gatattcaaa
    65761 tcctagagaa tatgcaatag aagctagtgg ctccaatatg ttagttatct ttgattctat
    65821 tctagcattt gctgatttta tatccattct tgttgagaat atacttctat gaaccctcat
    65881 atatttccca tctaaaaatt ccccttttat tgtatctata tcctcatttt tttctagctc
    65941 tttaaatatg ttttcatatc tacttaagaa aaattttctc tctggataac attcttttaa
    66001 tttatatata acttcaaata tattcttttg aattggcatt tggtcatgtc catttggaag
    66061 tattatatgg tcagtagttg cacccttatc aagaaccggt aaatatttat ccattcttgt
    66121 tcttagctca tcttcattca ttggtagata ttttcctatt gcataaccta atggaagtat
    66181 ttgaaccaaa acagaagacc catcatcact cttccaatta aactctgttt tattagttcc
    66241 ttttctttca gaacatcctc tccaaaacat tgagtatttt atatcaaacc catttagtat
    66301 ttgtggcatt tgagcacttt gaccaaatga atctggtaaa tatcctattt tcatatattc
    66361 tccaaattca tcacaatctt ttattccata tagtaaattt ctaagtatgg attctccacc
    66421 aactaccatt tcatcagttt gagtatacca aggacctatt attagccttc cttcttgaac
    66481 taattttttt attctctcct tacattcagg cttaactgct aaataatctt ctaatatagc
    66541 agtttgacca tccatcacat aatatggata gttatgatta ttttctaaca tatccattat
    66601 ttcttccata ttatttacaa gtaatattct agattcctca gttgagaagt accattctct
    66661 atcccaatgc atatgaggaa ctatgtgtac attttttttc attgcttatc ctccaaaact
    66721 tatctgataa tattttctgt ctaattaaaa atccaataaa aatactactg agtttacaaa
    66781 attttttaat atttatttta tcactataat taaacctttg atttaaatat ctaataacgt
    66841 atcttttaat gtatctttct ttgatttttt tgtttcctag ttattattaa taatattgct
    66901 gatattatag ctccaagtac tgccgcaaag aaccatattg atgctataat aaatcctgac
    66961 atacttccct ctaaaagcgc caatgagaat atccccgctc caggcacttg aagacctata
    67021 ttgaagaatg ctactataga cccagttatg gctgaaccag ctaccaatga tggtataaca
    67081 cggattgggt cctctatcat aaatggtatt gcaccttcag ttattccagc caatcctaat
    67141 atccaagttt gctttcctat ttccttttct gattctgaaa ataaatctct gcctaaaaca
    67201 gtagctattg taactccaaa agctgaaacc atcttaactg atgcaaatat tgcataaggt
    67261 ataaaatttc cacttgctat tgctcctatt gcaaatgtat aggctgcttt atttattggt
    67321 ccacctaagt caaatgaaac catagctcct attattgcac ctagtagtat tgcatttact
    67381 ccagtcatcc catttaacca atttataaga ccattgttaa gtgctgctac aggctttcct
    67441 actacaaata acatcaataa acctactacc aaagaaccaa ctactggata tacccaaaaa
    67501 cttacgaatc cagctaatgt gcctttggct ggtatattct tctttaaaaa ttttactata
    67561 tagccagcaa gtaaacctcc taacatacct ccaagaaatc cactacctat taaatcagct
    67621 gctagacctg ctgcaaatcc tggtccaaga cctggttttt cagctagtga gtaagacata
    67681 tatgctgata atactggaac catcaatgta cctaacatac ctccagaaag ctttcttaat
    67741 tgccataacc aagaaccttc ttggttgtat agttcttgta gaccaaatgc ttgagatgct
    67801 aatactgcaa atgcaagtat catacctcct gccacaataa gtggtattat atgagatact
    67861 cctgtaagta ttgctgattt tacatctttt ttccatgatg atttttcaca atatgatata
    67921 tcagatgatt tttctatata atcaccttta ccttgttttt gtgctacttc taaagcttcg
    67981 tatattattt cttttgctct ttttagtgga gctgctactg acgtttttat gtttggcaaa
    68041 tgttcaaatc tttctgtatt tttaggtgct acatctgttg catatattat tgcatctgcc
    68101 ctttttatta tatctggtgt atgagggtcc accataccat tagctccttg cttttctaca
    68161 tacacatcca caccgatttc tttaccagct ttaattaatg cttctgctga catatatgta
    68221 tgtgctatac ctgcagggca tgctgttatt gctaatataa ttttttttga ttcattttga
    68281 atcctaatct cttcttttat ttgttctttc ttttctttta tatctaagtt attaaataac
    68341 tctttcttag tttttgaatt taacaacata ttcatataat cttcatttga cagtctagta
    68401 actaactcag aaagtatttg tatgtgtgta tttccacctt cattttctgg tactgctatt
    68461 aaaaatatta gctctacttt attatttggg tctacgcttt cccaattttc tattggtgtc
    68521 tctaaggtca tagctgcaaa tgaagcttct ttaactgact tagattttcc atgaggaatt
    68581 gcaagccctc tttcaaaacc tgttggagat aaagtttctc tgtacattac agcttctaaa
    68641 tattcctcat aagatgttaa cttgccttca taatatagct ttcttgctaa gaactttatt
    68701 gcgtcttcct ttgtttttat tttttgtttt aaaaaaacaa ggttttcatt tgtgattttc
    68761 ttaaattcca aaattacgcc ctccttaaat catttaatgc tatgaatata gcataaaata
    68821 aattatgaaa atgttttttt gaaattatac acacaatttc ttcttaaaaa ataaagattt
    68881 gtgtatttat acacaaatct ctatcttaac cagtattgct ctattattgc tctcataatc
    68941 atcataattg gaaccttatc tgatatattg tacccattta cttgtttaat tgttgaatag
    69001 caatataaac ttatatctgc aatttgttgc aattgatttt cacatatatg tgttagagct
    69061 actaccttac aacccttatt aactgctgtc tttgctgctt ctataacttc tttcgtttct
    69121 ccacttaaac taagtgcaaa aaacatatct tgttcaccta atttccctat atcatgaagt
    69181 acttcatgtc tttgactata gtaagttata ttatcatgcc cacctctaat ttcttttact
    69241 aatatctcac ataaaggaat gttttgacct attccataaa ctataatact cctagatttt
    69301 tgaattatat ctgctacttt atttattttt tcataatcta ttagctctaa tgtctttttt
    69361 atatctgatt caaaaccatt ttttggctct ttatcctcat tttttaactc ttcttttaaa
    69421 ttatttttta gttgagagaa accatcatac tccatttttt tacatagcct aactatggta
    69481 tttgggacag taaaaaattt atctgctaaa gattgtattg ataattttac tatttcttct
    69541 ttgttttttc tcatatattc aattatcatg tcttctgtat ctgtaaattt atactcatat
    69601 ttttttgctc tttcataaaa ttccatagtt tctcctccat ctattttatt attcataagt
    69661 aaaattcaaa aatattagct aaaattttcg ttaatataat tataatttaa tatttttaat
    69721 ttattaatgc atactactct ttttattata cataaaaaat gtgtacttcc tatattgata
    69781 gagatgattc aagtttttat tttgatacat ctctatcaat atttttttag aaaatacaca
    69841 tttttttcaa atacttattt ttaattaaaa ctttaggtaa tctattcttt atatataatt
    69901 attagacttg aaaatatctt taaaatcaac caattcaata ttatttcttt ttaaccacat
    69961 tttaacttcc ttatcacata gcattgcatg ttcatatact ctctcttgaa taagagaaga
    70021 tgattttata agactataat ctataaatcc tggatgagta accatcaatg ttatatcttt
    70081 atctaaattt atatattttt cttcaaataa tttaacaaaa gatttattta acttgtaaaa
    70141 atcattctga ggatatttac ttggcatgct aatacatgaa tccaaataat agggacaact
    70201 tggtataccg tattcttttg cagcatcaca cattgctgat attgatacat cttcttctat
    70261 accatgtata tcaatatact caggcaattt actcatatac aacttgtaag tttcaatttg
    70321 agcacaaact tcttcatata cttcttcata attaaaaacc tctttacctg tcagtagttg
    70381 cgttcttctt attttgctag aaacaaattt tccaccttca tctaacaaat tctttatctt
    70441 aatatgttct ttgcaacaag gttctcctat tactaaatta acatgtaaac caaaacatac
    70501 atcatcattt tttaccattt ctatagcttg tcttgaaaaa ggcatattca ccatgattcc
    70561 agcacttgta acaataccgt tcttataaga agatattatc cctagtgtaa caccttctga
    70621 atatcctata tcatctgccc taactactag tttcttcatt ttatgcgtct gcctgctttc
    70681 tcaactcatc aagttgcatt tccatctcat cctttacttt aacaacatcc gttcctatga
    70741 tgatttgaac tgatgtatta ctcaatttaa ttaccccttt ggctcctgtt tttttaatat
    70801 tatcatcatt caaaatagta gcatccttca cttctaaacg taatctcgtc atacaattat
    70861 taagagtcac tatattttca taaccaccac aatttttaat tatctgttta gccatatatg
    70921 aaaaattacc atgagaaagt tttaaattct tttctttttc actaacttta tcacctatct
    70981 ctatctcaac ttcacgtcct ggtgtttgca cattatcttt tattattata tatttaaatg
    71041 ttacataata taatacaaag aatacaactc ctacaggtat aactaaccac gctttatctc
    71101 cataactaaa acttaagaca tagtcaatta tacttgctcc attaaatgaa cccaagcgaa
    71161 cacccagtaa atatagtaca actccaccta gaccagaata aaaagaatga attatatata
    71221 actttggaga tgcaaataaa aacgaaaatt ctattggttc agttactgtt gccataaaag
    71281 aagtcaatgc tccagcggtc attgcacctt ttacttcttg cttcttttta tcttgtgctg
    71341 ttttatatat agcataagaa atagcaggta ttgaaaacat cataattatg taaaatcctg
    71401 tccagaagaa tcctgcattt gggtctcctg ccataaatct aggaatttct cctcttataa
    71461 cttctcctgt tgatgttgta tattcaccca tttcaaataa tatgtaagca tttaaaactt
    71521 gatgtaaccc agttggtatt agtgcacggt tacagaataa aaacaagaat attcctattg
    71581 caccagctcc tcccaaccaa aaaccaaatt gattcattgc agcttgtgga tatatccata
    71641 ataaactaaa tatatatgat aaaattaaag caaccactgg tgatagtgta attacacatt
    71701 tttctcctgc aaagaagctt aaaaatgctg gaactttaac tttatatgtt ttattgtaag
    71761 tccatgctat acaagctcca acaattatcc caccaaatac acctgcattt aatgtatcat
    71821 tcaataactc taaatttgac ttaaatatca tatatgcaac tacggctgca accattacac
    71881 ttgtcttgtc tttagacttt gagtaggcac tagctgctcc taataaaaat aagtagtcca
    71941 catttgaaaa tactatatta gatatattag atatagctgg cacattgagc atatttggac
    72001 caaataataa tcctaatata cctgctgctg gcattacagc tattggcact agcatagtct
    72061 ttcctaaaag tgacagttgt tgcatcattt tgtttaagaa cctcttcata tccaatcccc
    72121 tttctatttc ggtttaattt tttgtgaaaa cgatttgttc acattttata ttttaaatat
    72181 tatatttgtt taaacaaatt gtcaatattt tttattcatt ttatttttat catttaattt
    72241 aacatataaa tccttttaag tgtattgcta tatctagtct atttttttaa ttcatttttt
    72301 ttattttaaa aatatgttta accatgtatt tgaaacaacc cacaaaataa ttctattttg
    72361 tcctttttat ggttgatatg attctatttt cactatacat gtctaaacac atttgatata
    72421 atttgttcat atcagttcaa taatattact aaagaccttt aaggagggat aggggtatat
    72481 ggctaaaaat aaattttcag agatatatga agtattgaaa gaggaaatac ttgatggtaa
    72541 atatacaagt aatatgatgc tgcctactga acttcaactt attgaaaggt tttcatgtag
    72601 cagaaacact gtacgtagag caatttctca attaaacaca gaaggatatg ttcaaagtat
    72661 aaaaggcaag ggtgttgtag tgttagaaaa tagttgttct aatgattttt ttctaaatat
    72721 gcacaatttc aaaggtgtag aatcgattgt agaagataaa aaggttaata ctgctactag
    72781 tgttctccac ttcagcaaaa tcttaattga taataagtta tctaaaaaga caggttttaa
    72841 agttggtagt gaagtatatt accttcacag actaagatat attgataaca tacctaaaat
    72901 tctcgacatt aactattttc tatgtagtat agttaaagac cttgatgttt ctatagcaca
    72961 aggctctatt tataaatata tagaagagtg tctaggtaca aaaattgttt catccagaaa
    73021 gatatttaaa attgaaaaag ctacagagct tgagttaaaa acgctacctc taaatgatta
    73081 taattgtgtt ggtgttatta aaaatagtgt atatactgat gatggaaagc tttttgaata
    73141 tactgaatca aagcatacac ctgaaacatt tgtattcatg gatgttactc aaagatatta
    73201 aaaaaattat gtttaattaa acataatttt tttacgttta gacactttat cccatactaa
    73261 cctcactatt ctatatacaa ttatatgttt atacaaataa ttatttattg tatattttag
    73321 ctttaatatt agtttaacgc ttgacaattt gtttaaacat attataatta acttataaat
    73381 tacaggaggt atcaagtatg aaaaattggt ggaaaaaagc aactgtctat caaatatatc
    73441 caaaaagttt caaagatagc aacaatgatg gtataggtga tataaatggt attatagaaa
    73501 aattagatta tctgtattct ttaggtgttg atttattatg gttgactcct atgtacgttt
    73561 ctcctcaaag agataatgga tatgatatag aagattatta taatatagac ccaaaatatg
    73621 ggacaatgag tgactttgaa aaactcttaa aagaagctca taaaagagac ataaaaataa
    73681 tgatggatat ggtcctaaac catacatcta ctgaacataa atggtttaaa gaatctaaaa
    73741 aaagcaaaga caatccatat cgtgattatt atttctggaa agatgctaaa cctgatggtt
    73801 ctgttcctaa taactggata tccagattta gtggaactgc ttggaaatat gatgaaacca
    73861 ctaaccaata ttatttacat ttatttgaag aaactcaagc tgatttaaat tgggaaaatg
    73921 aaaaggttcg tgaagaatgt tacaaaatac tagaattttg ggctgataaa ggtattgatg
    73981 gttttcgttt agatgtagtc aacctactct ccaaaacacc tggattacct gatgacccta
    74041 taacaggacc aaaaggagat ggaagaactc actacgctga tggtcctcgt atacacgaat
    74101 atttacataa tatgaatcaa aaagttttca aacctaaaaa tatagttact gtaggtgaaa
    74161 tgtcatctac tacacctgaa gagtgtatca actatactag agaaaatcga gaagaactat
    74221 ctatggtttt tagcttccat catatgaaaa cagattataa aggaggcgca aaatggacta
    74281 atgaaatgtt ctctctggat aaattaaaaa aagcacaatc tgactttcaa tataaaatgt
    74341 atgaaggaaa aggatggaat gctctatttt actctaatca cgaccaacct agagctttat
    74401 ctagatttgg agatgataga ttttttcgtc aagaaagtgc taagatgtta gccataacat
    74461 tatttggtct acagggtact acatacatat atcaaggtga agaaataggt atgacaaatg
    74521 cctactttac taaaatagat gaatatgatg atgctgaaag taagaatgca tactatcata
    74581 tgattaaatc tggtattgat gaaaaggagg cattgcttat acttcaacaa aaatccagag
    74641 ataatgcccg tactcctatg caatgggata atactattta taaggggttt tctaatcata
    74701 aaccgtggtt aaaagttaat aatgacaata ttagtgtaga aaatgagctt aatgattctc
    74761 gttcagtatt ttatacttat caaagattaa tcaaatatcg taaacaatat gatattttta
    74821 cagaaggtac ttatagattg ttagatgaaa atcataaaaa tctatttgtt tatgaaagag
    74881 agtataaaaa ccaaaatctt cttgttataa gtaattttac tcatgataat gtagtatata
    74941 aattacctga aacttatttg aataaattaa actacacaat acttgaaacc aattacacta
    75001 gagatataat tgaaaataaa atggaaataa aaccttacga aagtataatg atatattata
    75061 aataaagtta tttatgtaaa aatcggtata acaccctggt gttataccaa ttttactaaa
    75121 ttcaaagtaa tatttataga atatctatag attacaataa ttatttgact tctacatcat
    75181 tatatttctt tgaatatact ttagtcatat agaaatatat aggaactaca attatcatcc
    75241 cacctaatcc ccaaactatc ttcataggtt catcaatacc tgcttttatc agtaataata
    75301 tacttataat aactgcaaat attggtataa ttgctcctcc aggtattcta aaatttacat
    75361 ctttaccagc atacttcttt ctcaatatta gtacagctaa acatgtagga atatattgaa
    75421 taaatctaga tacaacactt atttgagata atgttgtgaa tgaacccgac catgctatta
    75481 gtaaagttcc tatgacagat actataattg ctatataagg agatccattt ttattagttt
    75541 ttcttataga cttaggcatt aatccttgtt caaccaaagc agcagcagac cttggtgtaa
    75601 atatggatga tgcaatattt atacctccaa cagatactat agttcctatt gtaattatat
    75661 acccacctat aggacctaac atcttaatgg cagcatctgc aacaggagta gctgaccctg
    75721 ctaagccatt acccaacacc cctatactta ttcccagtat aagcatatat attactgata
    75781 ctacaaacat taccattacc aatgctttag gtacattttt cttagggttc tccatatctt
    75841 ttgctgcaac agctaaagat tcaaatcctg taaaagcata aaataaagtt atagatactg
    75901 ctacaaaagc tgatgacgca ctttgacctt tttgtattat tacaaatggg gtaaaattag
    75961 aaccctttat aaagaataat cctacagcta taaataaaac tattggtaat aattttccta
    76021 tagtcatgat attatttaag tgcttagatt gtttcattcc cataaaattt ataatcccaa
    76081 gccctacaac taatatacca attattatat ttttaataag tggttctgct acttgtggcc
    76141 atacagatga tagtgttaca gcaaagaaat ttgccatagt agcccaagca atcatactaa
    76201 ctatccactt cataaatcca acttcaaatc caacaaaatc tccaaatgcc tcttttgcat
    76261 aaagataggg ccctccatca gtatcaaacc aactacctgc ttcagcaaaa cataaagcaa
    76321 tagaaacagc taaaagcata caaaaaacta atactaatat acttgctgga cctatatctg
    76381 tataagcatc accaggtaac ccgaatatac ctgaccctat tattgcattg attcctaata
    76441 gtacaatact ccaaaaacct aacttaatgc ctttttccat tgttattccc cccaaagcca
    76501 acttaatata tttatatttt ttatttagat gcttctttaa ctattgccat aaatattttt
    76561 ttcatattat cataatctct agtcatcatt tctggatgcc attgtatccc tattacaaac
    76621 tcacttcctt ccttttctat agcttcaatt aaaccgtctt tagatgtagc tgaaacaata
    76681 tatcccggtg ctactttgtt gacaactaag tgatgaaaac tattaactaa aacttctttt
    76741 tctcctagta tttcatataa ttttgtcccc tcctttatct gtactgtatg tgtaggtaca
    76801 ttagatagat gttgctgatt gtgtttaata taagcacctt caattaaaga taaatcttga
    76861 tacaaactac ctccttcagt tacatttata atttgttctc ctctacatat acctaaaact
    76921 ggttttttca tatctaaagc atgttttaat aatttcatat caaaactatc tctctttggt
    76981 gatattgccc ccaacttatt atgaggttct tctttccata tgagtggatt tacatcttgt
    77041 cccccagata gtataagcgc atctatatta gatacctgct cctttataat ctcatcatca
    77101 tatacaatcg gtacaatata tggtatagct ttacacataa ctactgattg tatataatca
    77161 ttatttacat aagccctttc atagccagga aacattcctc cttcatcaat tagtaaatta
    77221 cctgatattc ctattattgg atatttcttt ttagccaatt tcttcacccc ttaacttatt
    77281 tgtgaacaat aaaaaatgct acttctctta aagagcaagt agcataccaa gattttattt
    77341 atttgaacat tataatttat tttagaaata ctttttttga atacgagtaa tataaataac
    77401 tttatttact tatattttat tttaatcctg tttagtttga cttttttaaa ttgttttaag
    77461 tacaactaat aggtaaaata ggcatttaga ttttcctttt tatcaaccta ttaaatactg
    77521 tttaatatat tttttcccct tctatttaat tcacttccct ttctaccaga ttcacatatt
    77581 atcaaatcca gctcttttaa catattaagt attcctctta cctcaccttc tgttatattg
    77641 tagcctaatt catttattga attaacaata ccatttctac ctatattttt attaagatta
    77701 ttggcatttt ttaaacactc cattactaac tttactttct ctatagatgt tctatccttt
    77761 aatatgctta tttctttact aaaatcttca gttatattta ataagttatg aggtaaatca
    77821 tttattaaaa taacatcctc agaactcata atgttaatat acatagcagt attttttaat
    77881 tctcttatat tacctggcca ttcatatgca ttaataatgt cttgaacatc ttgactaatt
    77941 gctctcttcc ttttcataaa atgctttaac ataacttcta tatcttcttt tcgctctctt
    78001 aaaggtggta tatttatggg caatacattt atcctatagt ataaatctgc tctaaatcta
    78061 tctcctttta tcatttctaa taaattttta tttgtagccg caattactct aacatctata
    78121 tctattacat tttctcctcc aaccctcatt acttgattct cttgtaatac tctaagtaat
    78181 ttgctttgta agtatatagg catgtctcct atttcatcca aaaatatagt tccattgtta
    78241 gcctgttcaa ataatccttt tttacctcct tttaaagctc ctgtaaaagc accttcttca
    78301 tatccaaaaa gttcactttc aagtaaattt tctggcatag ctgcacagtt tattgctata
    78361 aatggctgac tatgtcttgg tgatgcatta tgaatagact gagccatgag ttcttttcct
    78421 gttccacttt ctcccgttat aagaattgta taatcagatt cagctatttt catacctaat
    78481 ttcttagttc tttccatctt cgttgaaact gtttttatat catcaaaagt atatctagca
    78541 acttgtccct tttctctaag cttctttgat aggttttgct ccaattttct tatgtaagtt
    78601 atttcttgaa tacaaaaata atatccagta acccttccaa aactctctat attatactta
    78661 tttacgttta agtacttatt attaaatttt ataacttcat catgtgcctt actcaaacta
    78721 aaaatctttt taatattctc attctctaat tcatctatat ttttccctat aatattactt
    78781 tgaatatcta gtatatccct taaactatta ttacaaataa ttatgtttcc cttatctgat
    78841 accattgcca tacctaagtt tgataaattt attatggaat ttagttcatc tgcttttata
    78901 gtaagttctt tgtatttagt atttattcct gaatataaac taattatttc atctgtatac
    78961 ttaattagcc tctcagtaat ttctttatca tccagtttga gttttgctat tatttgaata
    79021 aacgttgaac tatcaatata cctatggcct aaatcaataa cttcttctat atattttgga
    79081 actaaacttg actctcctgg cgttatagct atcttaatat ttctatattc ttcattggga
    79141 ttgtatggta taaaattcaa atgatttatt cctatattgt ataaattaga tattgtttcc
    79201 aatacagtat gcttattatc gtttactact aatacatcta ttccttctgg caatgaaaat
    79261 aacttatata tatccttttg ctttatgctc ctatttatag ttattatctt tgagacatct
    79321 tccacatatt tttttatttt taatactcta tcatctatca ttaccaatat tatgtcatct
    79381 tttattaatt ccttctctgt tattttattt aaataataat tttttatcat tactttatct
    79441 ttaaagatca tttctagatt tttatttaag aaacattcta atttagacaa cccatcagtt
    79501 actactgcta ttgttttcat ttgattactc cttttaaaat atactctact cttttataat
    79561 atacatttga ttttcacttt aataacacaa aaaattgtac atttagtata ctaacatatt
    79621 cctttattag ttttatgtcc ataaatatca tagttgcaat atctatcata tttcttaaac
    79681 ttctataaag caaaaattgt gccaaattaa taattttctt aattttattc taaagttcct
    79741 caatagtatt tacagactta gcacttatag cttataagtc tttatattat taatctattt
    79801 ttataggtta attgtacaaa tcatatatca cttttaccta ttaaatgcat tataaaaaat
    79861 tgtatttata aacactattt actactcttc taagtacaaa cagtatctac atactataac
    79921 ctttcaccat tagattctat tacttgttta taccagtcaa aactcttttt cttaatccta
    79981 gacaagtctc cacttccatc atcatactta tccacgtata taaacccata tctcttggcc
    80041 atctcaccag tagatgcact tactaaatct atacatcccc atggagtata tccaaataaa
    80101 tcaacgccat cttctacagc ttccttcatt tgctctatat gtgcttttaa atactctata
    80161 cgatagtcat catttatcac tccattagat tcttttttat cataagctcc taaaccattt
    80221 tcaactacca tcataggtat tctatatcta tcataaattt catttagtgc atatctaagc
    80281 ccttcagggt ctatttgcca tccccattca ctagacttaa gatatggatt tttcactcca
    80341 cctaatatgt tgccacttgt attatcttct ttagaatcac tacttacaca tgtagacata
    80401 taataactaa atgtataaaa atctacacaa ccttctttta aaatttctaa atctcctttt
    80461 tccattttta tttcaatatt attttctcca aaatatctat tcatataagt tggatattcg
    80521 ccacgcactt gtacatctcc acagaaataa ttcattactc tactatctct ttgagtttta
    80581 actgcatcat ctggattaca agtataagga tacattgttg caaatatttg catacatcca
    80641 acttgataat ttgaatctat ttcatgagct aacttaacag ccttagcact tgctacaaat
    80701 tgatgatgta acccttgaaa acgaagttga ggtatatcta cttggtttat aaaatctgta
    80761 gtcccttcat ttaatattcc ttgtgataag tagcctccca taggcattgt agctgaattt
    80821 atttcattaa atgtcagcca atattttact tttcctttat atcttttaaa tattgtttca
    80881 cagtaattca tatacaagtc tattacatct cttgaaaccc atccattgta tttctctgtt
    80941 attgcaaatg gtatttcata gtgagatatt gttattaatg gttctatatt atattttaaa
    81001 cattcatcta tcacattttc ataaaattta agtcctgctt catttggttc tttctccatt
    81061 ccagttggaa atattctagt ccatgctatt gaaaatctaa ataccttaaa tcccatttct
    81121 gcaaataaag ctatatcttc tttgtatctg tgataaaaat ctattgcttc atgacttgga
    81181 taaaatgttc ctttttctat aactctcgtt attcttttac ttactttttg agaacctcca
    81241 gtacacatat ctgaacaaga aggacctttc ccatctaaat tccatgctcc ttcacattga
    81301 tttgctgctg ttgctccacc ccataaaaaa tcttttctaa gtgccataat tctacctcca
    81361 ataatttatt tatagtaact ttattttaaa ataaaaatac atatatataa ttcttaatta
    81421 atagaactct cttttacgct aaaacagtta tcaatttctg actattattt acatgctttt
    81481 gtgtattttc tacaatatcc acataatcag aataattagt aacaactact ggtgtttcta
    81541 agcaatatcc ttcttttgaa atactttcta tgtcaaaagt tattaatgtc tgaccttttt
    81601 taatcttatc cccttgatta acaaaagatt tgaaatattt accttccaac tgaatagtat
    81661 ttaaccctat atgtatcaat agttcacaac cattatcaga tataattcct attgcatgtt
    81721 ttgttggaaa taaagtcatt acagtcccat caaatggtgc cactactttt ccttctttag
    81781 gtgttattgc tacacctttt cccaatatac ctgttgaaaa cgctgcatct tgtatctgct
    81841 ccaaagcctt tacttcacct tttattgggc tatatactac ttctcttttg tttttttcta
    81901 taacgccttg cccattatca tctttagaaa tttctactat agcatcatct ttccaaaaaa
    81961 ataatgttaa aacaaatcct actacaatag ctactattat tgctattata gttgcataaa
    82021 cacctttcat atctccagtt gttggattta tataattagg tataccaaat attccaagtc
    82081 cacccattac ataacttttt acattcatta tccccattac agctcctgcc acagcaccac
    82141 ctacacaact atatatgaat ggtgtttttc ttggtaaagt tattccatat attgcaggct
    82201 ctgttacacc acatatccct gaaataactg caggtataca cagctctttt atcttcttat
    82261 cttttaattt aaagtacatt gctgctacta ctgctgtttg tgcaaaagat ggtacaaatg
    82321 ctcccactaa tattgtgtca aaccccatag tcataaggtt tgacatagct attggtacta
    82381 atccccaatg tagaccaaat attacaagca cttgccacaa taaaccagtt aatgcaccac
    82441 ataaaattgg tgaaaagtta aataaagcta aaaatccatt tccaactata ttagaagcat
    82501 aggtagctat aggccctata accataaatc ccaatggtaa agaaactaat aaaactgcca
    82561 ttggtacaaa gaaattcttt actacatctg gtattatttt agcaaacact ttctccaact
    82621 tagctactaa taatactatt aaaattactg gtactactgt tgaagtgtag ttcattgcta
    82681 ttactggtaa tcctaagaaa gttatatata ctggtgattc aaatattgtt ccagtaaata
    82741 gattgtataa aggttgacct gtagatgcta gagtatctaa ttgaatacct gaatagcata
    82801 aaactgcacc tataagcatt ccaataaatg gttttaattt gaatttctta gcagctgtat
    82861 atccaagaat tattggcata tactggaaca ttgcatctcc aattgcattt aacataatat
    82921 atgcaccatc agtttctcca tacaaaccca atgctgtaaa tactgcatta aatcctttta
    82981 tcataccagc agcacacaga actcctaata ttggttgaaa aactcctgat attatgtcta
    83041 taaacttatt aagtaatcct tttggcgcat ctgagtctga attatctatt gtatctcctc
    83101 ctaatccagc caaatcgcac acaacttcat aaacatctgg aacatgatta ccaataacta
    83161 cttggtattg acctgcactt ttcattacag taactatacc atccattttt tttagcacat
    83221 catcatttgc tattgactca tcttttaatt taaaacgtag cctcgttata cagtgagata
    83281 aactgctaat attatccttt cctcctacat tcgcaatgat ttcttttgat agttgttcat
    83341 actttctcat tttttctcct ccattttctt tttgaaatat gaaggtttgg ccaacttaat
    83401 gtcaccaccc aatatatata aacctaatta ttaggattta tatctgactt aatactttta
    83461 cttaccactc tttcaacgtg aatagttaag tataattttt cctcatctgt taaatcgtag
    83521 ttgtattttt tatgtataaa ttcatccaac ttttctatga atttataagc atttttatat
    83581 ttaactttta ttacatttaa taaatcatct tcattatttt catgtaaatt attactaaca
    83641 attcgttctg caaaatactt taagtgagta ataaatctat gataataaac tgaatcttca
    83701 tcaaatacta ttctgtaatg gtatttaacc atatttgaaa tttcttgtat tatttgtgta
    83761 atctcatata tagtttttat actttgatcc atttgtgcat taactatatg cagtgctata
    83821 aatccagctt catcttctga tagtctaatt tcaaattttt tttctatata atccaatgcc
    83881 tctaacccta ttttaaattc actcttataa aatcttttta tatcccaaag taatacattt
    83941 tttacactta ctccttcacg agctctttct attgcagtat acatatggtc tgttaaagaa
    84001 atataaatac tatcatttaa tcttttccct agtttttctt tagcatataa aattatttca
    84061 tctgataatt ccatatatct tataggaata tctgctatta gttctttaaa tttatcagat
    84121 atatttgggt ctgaaagttt gaatatctta tcaattttat cctttgatat atgctctcct
    84181 gtacgttttt tataagctaa acctctcccc ataactacta tttccttatg attttcatca
    84241 gttgtaataa ttacattatt attgagtatt ttttcaataa tcattgtgat gcccccttta
    84301 ttagcattaa gttaaaataa atctttttta attatttaga atatttaaaa tgtaatttat
    84361 tacataaaaa aaaacctatt tattacacat taaatagact cccatgagtc ctgagtaata
    84421 aataggttta gcttgtattt aaacaatcac tatccgtttg tattttattt taattattat
    84481 ttattatatg ttagcaaatt tattttttag tgtcaacttt ttctcaatat tttaatttta
    84541 aataaattat ataaattgaa tcttcaaagt aatattttta tcaataaaca aaataaaaaa
    84601 ggtattactt tctttaagta atacccattt atttttaatt taagtggttt ttcttaatct
    84661 catcaagtag tccatcttct aaataaattt ctaatgcaca tcctaccatt gcctttactg
    84721 ctttgtgaag tttatcataa gcttcctcag aatttgcata atttagaaat tctttttcat
    84781 gaacatatac cattggactt atatctaagg ctatttctgt atacatagta gggcaaactt
    84841 gacttacatt acctatatct gtagaaccta ctggaccatc tttaccttct aatatatctg
    84901 ttatacctgc ctcaatcaaa ttattctttg ttatcttttg aagtactttg ttatttatta
    84961 tgttgtcaaa agaattttca aagtatctat aagaaagctt agctcctgtc attaactctg
    85021 caccttttgc acagtttata acttttttag ttacttcatc taaataactt ctttcagcag
    85081 ctcttacgaa aaacttacag gctgcttttt ctggaactat atttggagcc tctcctccat
    85141 ttgaaactat accatgtatt ctaacatctg attttacatg ttgtcttagt gcatttatcc
    85201 cagcaaaagt aagttgtact gcatctagtg cattaactcc catatgaggg tgactagctg
    85261 catgtgctga taccccttca aaactaaact ctaatgaatc tatcgccaaa gccttacagt
    85321 ttatattatt aaaagcttct atatgcattt gataacttac atcaacatca tcaaaagctc
    85381 ctgccttaac taaatcacat tttcctcctg ttgtttcttc agctggtgtt cctattagta
    85441 caatttttcc tttaaaattt tcttttaact tacttaatac tatacaagct cctagtgtag
    85501 atgctgcaat ccagttatgt ccacaagcat gagctggttc tttattttct ccatatccag
    85561 gaagtgcatc atactcagct aaaaatgcta tggtaggtcc atcattatct cctaattcag
    85621 ctctaaaagc agtctccata ttacaatatg gataaactgt atcaaatcca tattccttca
    85681 ttttttctac taagtatttt gatgatatgt attcttcttc ccctaattca ggattgttaa
    85741 atataaaatt acaaacttct tcaatttctt ttccaagtcc attctctatc tcataagctc
    85801 tcttctttat actctctatc ataatttacc tcctactaga atcctagata taatcctatt
    85861 gtcaaaaatg ccattacaag aattgttaat actaccatta aaggaattac gaatctaacc
    85921 cactttgacc aaggaatatt ggctactgct agtgctccca ttaaaacacc tgatgtaggt
    85981 gttatcaagt tagttacacc tataccaaat tgataagctg taactatcat ttgtcttgat
    86041 acatctacta agtcgccaag tggtgcaagt acaggtattg taagtgatgc aagccctgat
    86101 gctgatggta ttaaaaatgc tattaatcct tctattaaga aagttaaatt tataaatata
    86161 gtttttggta atccatttaa taaatttgct gcagaattta aaattgtatc tattataaat
    86221 ccattttctg ctattattac gattccacgt gcaaatgcta tacacaatcc tgctgtaatt
    86281 aaatctcctg ctccacttat aaatgaatta actatctcat cttgtttaag ccctgatgct
    86341 attcctgata ttatacctat agctgtaaat atcattgcaa tttcagttat gtaccattct
    86401 agtcttaaaa ctccatatat cataatcgcc atacctattg tgaaaatggc tattaccatg
    86461 gcttctcttt ttgtaaattc ctttatattt gtatcactat taaccattaa ttcttgattt
    86521 agtgatatat catgtactaa agatttttta gggtcttttt taactttatt ggcatacatc
    86581 ataacaaatc ctatagatat agccatatat attataaaca taacaactct aaactctatt
    86641 ccagaacctg gtactatttg tgataatgct tgtgctattc ctacagagaa aggattcgta
    86701 gtagctgctc caaatccagc tgttgctcct ataaatactg tggccactgc tgtcaatgaa
    86761 tcatatccca tactggtcat aagtggaata aatatcatat aaaaaggtag tgtctcttca
    86821 ctcattccaa acgtcgtccc acctagtcca aatagaatca ttgaaatcgg tattattaaa
    86881 aattgaaact tcttcatttt atttacagta tgagctataa cagcttctat agctcctgtt
    86941 ttattaacta tcccaaaagc tcctccaact attaaaacaa atccaactac ttcagctgca
    87001 tctataaacc cgtttatagg tgctgtaaat acatcagtaa ttccttgtgg atttgatggt
    87061 gctttttcat aagttccagc tacaacaact gaccttccgt ctatatctac tctttcaaaa
    87121 tttccacttg gcactatcca agtaagaatt gccattaaca ctattagcaa aaatattatt
    87181 gtaagtgtct gtggcatagt aaactttttc ttttttaaac tgctcatttt taatctcccc
    87241 actttttaaa ttaaatattt tattattgca tccctttatc ctattttgta tagatgtaaa
    87301 aaagctcaac atggatacat gttgagctaa atgtgctaaa tacaacatac ctgcatcttc
    87361 cctttatctt gtttaagata gctctccatc aaaattcggg ataattttga tgacagtact
    87421 gtacctatta aatacagtcc cagcactagc ttcggacact agcacttcgg cacttactcc
    87481 ttttatattg cctcattggt ttccaacctc ttcaatatta atcttagtaa atgcaacctc
    87541 tacctcaatc tgtttgttag atgaggtaat tcttattaat ttttattaat tcaaagtaat
    87601 tgtagcataa tatgtcgaga ttgtaaatat tttcagtata ataattctta ctttatgatt
    87661 tcattccatt tttcacaaat aattttttct ccatataatt taaatactgt agtatatttt
    87721 aaatacacac aaactttcca caaattttaa attattataa tacctttata ataaaaaata
    87781 aaagctatgt aaacttatca atttacatag ctttatatac attaatctac tccaagtaca
    87841 gctttaagct ttgatgctat tgtaataact cgtggaccaa atataacttg aactgagttt
    87901 ttctttttaa caactccact tgcacccaat tcatttaccc agaagccatc ttcatagaat
    87961 ccatcaattg aatttagctc taccctaagt ctagttgcac agtttgtaac attttttata
    88021 ttattacttc ctcctaaacc ttctattata gaatgtacaa gctttgtatc tttatcatct
    88081 acttcctctt ctactgcatt gttgtatgct gttttaccag catctacaga ttgtctttct
    88141 ctatattctt tcttactata aagcttaact tcttctgagc ttcttcctgg tgtttttata
    88201 tcaaacttct tgattgccca tgtaaatatt atataagtaa ctataaaaaa tattggtatc
    88261 aaaaataata atggtaacgc atccactttt tgtggttgta ataaattagg aaacatattt
    88321 ctaaatgatt ctcccattat agaaacatta aacatttctg taaatacata cgtcaatcct
    88381 gccagtggta catatgctac aaaatataat aatggtgcaa caaataggaa tgtatactct
    88441 attggttccg ttacccctac caacatggct gttactattg ctggtatcaa tatacttatt
    88501 gcttttttct tattagcatt atcagccaac ttatacatag ctaaagctgc tgctggtaat
    88561 attgcaaaat gtattaataa acgacctgtt gtgaagttac gagttatata agatagtgta
    88621 tctgggtctc ccatttgagc taatcttata ttattaacac cctcgtaaat tgctccacct
    88681 acttccattg ttccacctac tcttgtatat tctataggga atgcaattaa gtgatgtata
    88741 ccaaatggta ataatgcttt atccattgct ccaaaagtaa atgttcctaa taatccacta
    88801 gaacctatcc aattaccaag tttagataat gccaacccta ctggtggcca tataaagtac
    88861 attgctgtac ctaatactat tgatgctata aacatcataa tcattgaaaa tcttggacct
    88921 gagaaaaaag ataatgcact aggaagttct atgtcaacaa atttgttatg tactgcacta
    88981 gctacaaatc cagctattat agctccaaat atactacaat catatgaaaa gattcctaag
    89041 aaagaaccat ataaagaact tcgactatat gcttctgcct ctcctaatcc agatgccata
    89101 aacgcttctg ctgtaactgt atctgaattt ataccatttg ctgctaacaa tgtacttata
    89161 actgtgtgca ttcctataaa taaaacaatt cctcctaatg caccccatcc tttttccttt
    89221 ttagataatc caaaggctat acctgtaaca aagaatattg gtaagtatct cattaccata
    89281 aatcctaaat cgtttattac tttaaagaat acatttataa atgacccttc ttttattagt
    89341 ccgctaagcc ctaatgcaga aacgttttcc atatttgcaa aagctgaccc taatcctata
    89401 aatactcctg ctacaacaag taatataaca ggtatcatca ttgctccaga aagtacttgt
    89461 aatttatcct tcatagtatt atttcctcca tttttattga aatgccaggc tatataatgc
    89521 atatatatat acttctattg ctttgtataa tgtatctata cttatatttt catttgcctg
    89581 atgtgaattt ggattatccc cctctattgt gggtccaaat gcaactccat tttttaacac
    89641 tcttgcataa gatgcagctc cttttgtaaa aagttcagct tcttttccaa aaccttgttt
    89701 atatgcattt gataatttat ttattaattc actatctggt tttacataca gtaattttaa
    89761 atccttatat atatcaacta ttaaattttc tttttttcca atttcattaa ttctattagt
    89821 tataaactcc caacttatac ccttaggata tctaaagtct attttcatat tcaattcatt
    89881 tttctctaag aagatactca ttatacatat tgtagaaacc cccatatcta catcttcttt
    89941 atatatccct aatttttttc catgattatc atctgtaaag tatgaattta aaatatccct
    90001 tattataacg cctttatcat ttagtttttt aattttctct aaatcttttg ctaaactaaa
    90061 tgcacagtta tatgacctgt gtggatttct cgataaagcc ctttctcctg tatatcttac
    90121 taatacttta ccttcttcta gttcttctat ctctgtataa taagctaatg attctactaa
    90181 atctttctta tcattagttt taaaaacagc ctcaattttg ttacatacaa atccatcttc
    90241 tttgtttgac tttatatcaa ctaaattgtg atttctaaat ttatctttat ctattttctt
    90301 tcttagattg aagtataaaa tacctttttc tccatttact attgggaagt ttccatctgg
    90361 agaaaatgca aattttggct gttcattgac actaaagtaa tgctccatac attcccaagt
    90421 cgtctcttca gctccaccca ctatcaatct aatctttctc ttcggctttt cattaagttc
    90481 tcttaagaaa agtaatgcat ataaaatccc tatcaaaggt cctttgttgt cattaactcc
    90541 tctaccataa agatagttat ctttttgaca tactttaaaa ggtttactct tccaaagttc
    90601 tttattatat attggcacca catctatatg attaagcacc cctactactt cttctccttc
    90661 tccatattct atatgaattg catatccatc aaagtctttt acaacaaaat ctttgtcttt
    90721 tgctatttga ataagcttat cgaaggcatt tcttatttct atcccaaatg gttgatttat
    90781 atctgttgta ctttcatctc taattgaagg tatttcaact aaacttctaa tatcatttat
    90841 gatattttct tgattggatt ttaaataatc aataattagt tctttgttta ctatcatctt
    90901 aattatttat caccccttta tattaatttt acaaagcatt tcagttcata tctacattat
    90961 aaattttgaa tattattttt tcaatatttc aaatacttct ctaagttacc ataatgatac
    91021 ggtaaacaat caacagatta tgacaaaatc atactatacc tattactaat attttaactt
    91081 atctattacg tcttttttaa tgtaagttta ttccaaaaat acataaaaaa agatgtagcc
    91141 ttagctacat cttcttatat ctattcaact tccattatta tgctattaat atctacattt
    91201 ccaggttcta tcctatcttt tactgttctc ttaacagaat cagcatcatc cattataact
    91261 acaggagtta ttatatttaa atcatttgac gttataaaat ctaaatctac ttccattatt
    91321 aaatctttag cttttacctt atctcctgcc tttaccttaa catcaaacgg ctttccttct
    91381 agtgacattg tctctaaacc tacatgtatt aatatatttg tattatcatt actcttaatc
    91441 attattgcat gctttgtatc aaatacattg actatttcac catcaacagg agaatataac
    91501 ttaccttcac ttggtattat tgctactcca tctcccatca tcttttgggc aaaaactgca
    91561 tctggaacat cttctaagtt cactaaattt cccttaaaag ggctatatat tttctcaatc
    91621 ttattttctt ttttcttaaa tatattaaac ataacacact ctccacatta taaaatttta
    91681 tgataatatt tttcttaaag cttttgctac accatcattt acattagtat cagttacaaa
    91741 atctgccata gattttactt catctgtagc atttcccata gcaataccaa gacctgcaaa
    91801 tttaatcatg gatatatcat tttcattatc acctatacaa ataacttctt cgctatctat
    91861 acctaatata tcacaaaatt ttttaactgc aatacctttt gaagtacctt tgctcataac
    91921 ttcaaaattt atctttccag aacttactac ttctaaatct tcttgttttt taaattcttc
    91981 ctttgcttct ctcaaagaat ctacgttttc tgagaaaagt atacatttta aaatttcatt
    92041 atcagctttt tctaataaag tttcacaact tgcattttct attatttcaa ttctgtcttc
    92101 ttctttattt ttactattta ttaatctata tggatgttct tcacctattt cattgttgga
    92161 tataactatt ttacagcctt taaaatgttt gtttaaatta tacttttcac ctattgtata
    92221 gattttcttt agagtttctt tgctcaaaac cttcttatac tcatatccca gtaatttaaa
    92281 atatgtccca ttagttgata taatatatat atcatcccct aaaacacttg taaaatattt
    92341 catagcatta taaggtcttc ccgtaactaa ggctaccttg ataccctttt ccatgacttc
    92401 ttttagtgct ttaatatttt cttctgatac ttcaaatcct ttacccatca aagtaccatc
    92461 catatcagta caaattaatt tatatcgcat ttgtatcaca tcctagtaag atttttctaa
    92521 tatttcaaca aggtcttctt tctttccaaa aggtaaaaat cctaaaactg gacagcttgt
    92581 tatttgttct aattgttcct ttgttacatt aaactcagac atttttgtct taagtcccac
    92641 tctctttaaa aaactttcta tttcatttcc taaagtttta gctgcttcat catcttccac
    92701 tttttctaat tcttggttta gcattctcgc tactttggca aatttctcta tattatcctt
    92761 atagaacttt tctgcaaatt ttgggaacac tatagccaaa gcctcaccat gaggaatatg
    92821 tgtaactcct cctattattt cacttagtgg atgtggtgct gctgctccag catttgccag
    92881 tgaacttcct cctaatgtat ctgctactgc taactttgtt ctgtattcta tattattttt
    92941 atcttccatt actttaggta gatagttaat gactaattcc atagattttt cagacattat
    93001 ctctgtaaaa tcattagcat ttggatttat ataactttca aatgcatggg taaaagcatc
    93061 aaatgctgtt gatgcagtaa ttctctctgg cagagttaac ataagctctg ggtcaactat
    93121 acattctttt gagaaacaat tttgatgaaa tattgtattt ttctcgcttc ctctcgatat
    93181 tactgctgct tgtgtaactt ggcttcctgt tccagaagta gttgatacag ctattaacgg
    93241 taattctttt tcacttacct tatcgtatat tttaaatgga tttgtatatg tactaaacat
    93301 ataatcccaa tctatctttt ctaaaccaaa agtcaatgct aatacttttg cagtatctat
    93361 acttgaacct cctccaacag ctaaaacaac atctacagat tcttttttag ctaattcaaa
    93421 cccttcattt attaattcta cagttgggtt tggctctact ctatcaaagt gtacaacttc
    93481 tatacctttt tctattaaat tcaatgtaac tcttttatat aacttgtcta atggctctac
    93541 atttggagta gtaactaaca aacatttctt tccatatttt cctattattc taggtaattc
    93601 acttaaagtt ccttgtccaa aatttattct tgatggttga taaaagttaa tcatattttt
    93661 cctcccttaa attattgcct tcatattcaa attatacata tcataggatt tttaaggcaa
    93721 taccttcact gttaactctt atttactaaa gcttatggca attattagac aataaatgtt
    93781 ttattattac catatcatta tgtaaatttt attaagtacc catgttataa ttttataatc
    93841 aatcttattg tataattgac ttaataataa aaatagagta tatacacttt tttatgacaa
    93901 atagcttaaa ggagaattaa attatgaaat atttgataga cagtgctaat cttgatgaaa
    93961 ttcaaaagtg tttaaagtat ggatttaagg gaataacagc taatccatct atgtatctta
    94021 aagagaaagt aaacttccat gaatttattg agacatgtgc taatttaaat ccagaaatat
    94081 taactgctga agtcattgga aataatttat acgaattaat taattcatgt gataagatat
    94141 taaatataaa taaaaacact ataataaaaa taaatttctc tgaagttggc ttagaactta
    94201 taaacatatt aaatagtaaa ggtgtaaaaa ctgcatgtac cttgattttc aatgtaaatc
    94261 aagcatcatt agctataaat gcaggtgctg attacctatt cccttttata ggtagaaatg
    94321 atgaaaatgg gaatgatggt ataaaaaacc ttacagaaat atgtaacctt atatcaataa
    94381 atggatataa tactaaagtt gttgcagctt ctattaaaaa tgtattccac ttaactcaag
    94441 catctctttg tggatgtgat tatgcagctg taacatatga tttatttaaa aaagcttctt
    94501 ttaaccagct tacaatagat ggtgccaata cttttgaaag agattggtcc aaggttaata
    94561 aatagtaact aagatttagg agggtttgca ttgaacaaaa gattattaga tattgctgaa
    94621 ttattactca acagctcaga ctatattact gtagatacta ttgccgaaga attaaaagtt
    94681 agtaataaaa ctattcgtaa tgaccttgtt attttagatg aatggctttt agagtttaat
    94741 ttatccctag ataaaaaaac tggttcaggg gttattattc taggtaatga agatataaaa
    94801 cttaaagtta ttagagatat caatgataaa tcaaattgta tatatgcata ctctcctgaa
    94861 gatagaaaaa ggtatattct aagtaaatta tttattaaaa attctaagtt taggattcgt
    94921 gatatttgca atgaactaca tgttagtaga gctactgtgc ataaagactt ggtagttatt
    94981 caaaattttt tgcaaaactt taaagtgact ctagttagaa aaaccaataa tggagtatat
    95041 ttagagggaa aagagaaaga tattcgaaaa gcaatttttg aacttgctac tgttaataaa
    95101 agttatactg aacttaaaga aatattattc tcgaaatcta atgaatgcaa agatacccca
    95161 tcaacaaaaa tatttaagga gctatttaac tatgattttg agaaattatc tcaaatcact
    95221 ctacacacct tacaagtaga aaaaagtaac cttagtgatg agtattatat aaacttctta
    95281 atacatatag ctatatgtat taaaagaatt tcaattaaaa gatatattaa cttatctgaa
    95341 ggttttttta gagaattatt agaacatgaa tactttgaaa aatcaaaaat tttatgtgct
    95401 aacctaagtg aagcttttga cgtagaattt attgatgaag aggtctgtta catccttctt
    95461 catatagaag gattacttaa atcaataaag aaaactacta ctcttgaacc tcttaaaaat
    95521 gaagaaatta cagagtataa tctatctgaa gcaatagctg ttcattgggg aagtatacta
    95581 aatttaaact taaaggacga tttaatatta ataaagtctc ttgctaatca ccttaaatca
    95641 gttctacaca gaataaacta tgggtttact attacaaatc ccatattaaa tgatataaag
    95701 agaacatttc cttatactta taaggctgct aaagagtcta atactgttat caaacagttg
    95761 atgaattatg aagtcaatga tgacgaaata ggatatcttg ctttatattt aatatcagca
    95821 atagatagaa gcaaatctcc cctaaatact attttaatat gtcactgtag tgatggtgta
    95881 tctaatttat tagtccaaaa gttaagtttt gaatttaatc agattaatat agttaaatct
    95941 atacctctat cttctatttc atttacgaac tttgatgatg tggacttaat actaactact
    96001 gctcctgtag actttgaaca taatgctgaa ctgataaata taaatgctct tttaagtaag
    96061 aatgatattt ctagacttag tacaattata aaaaaactat attctgaaaa aaataaaata
    96121 ttatcttata aataaacaaa aataggcttg tctcaacata gagataaacc tatttttcat
    96181 tgtccaaatg ataaaaatat atttgatttc atagaaaaag tgcctcatga actaaaaaat
    96241 tcattttgag acaccttcat tttttatatt taatcttttt atttaagctt ttatattttt
    96301 taaactttta tatttaaagt aaattttatt atttttattg agcacaagca ttatttatat
    96361 tattaatttc ttcagtattc ttaccaaact ttgtagttac aaatcctatt gtttttatta
    96421 tgagtaataa ggatatcaat gaatatatag atatcataaa attcttactt attattccag
    96481 acgctactat tataaatcca tctagcataa ataatataac tggcaacttc agaaacttaa
    96541 ttaccttatt aataagcatt gcaactaaat cagttccacc agtactacag ccccttatta
    96601 ttgctatccc tatagcagtt cctaccaata acccacctat cactcctgct actaatagat
    96661 tgtcagttat gtgagtttgt ggtattactt ttaagaataa tgggcccata agagttatat
    96721 ataatgtttt tattccaaaa cctttcccta aaaataataa tcccattact aaaagtggca
    96781 tagatattcc taatgatata ttctcaacag gcattgataa gaatgtgcta agtacgactg
    96841 ataatcctgt tatacctcct ggagctaatg tatgttctgc aaagaaaaaa ttaactgcta
    96901 atgccattaa aaatgctgat attgttatca gtaaataatc ttttatcatt tcttttttca
    96961 tttaataacc tcctatgatt atattgtttc agtataaaaa atcaattaaa ttagtctagc
    97021 ttcctgtagt ttttcataaa attaatgctt ccttaaaaca ttaagaaagc atctctttta
    97081 tcatagttta ttatcttatt ctaaaccaaa ctttgtagct gcttcatcta tagcttttct
    97141 acaattctta acacctgctg ttataccatc tttatccttg aatattgctg aagataatat
    97201 tacaacgtct gggttacatt ctattaatgg ctccatattg tctgatctta gtccaccatc
    97261 tattgctaat tcacacttag gattcttttc gtctatcatt tttctagctc tttttactaa
    97321 atcaacagca ctctttctcc atccccagtt atcttttcca tcagtttcat caacaccatg
    97381 aacgactatt tgcaatctgt ctatatcata tatagcttca tcaacaaaac ataatggtgt
    97441 gtaacatcca attgttaatc ctactttcat accaaattct ctacagtaat ttattatgta
    97501 tgcaagagga gcacctaaaa aatgttctgc tggtattatc aacaagttac atcctgctgc
    97561 tgctattttt tctataaata atctatcaca atctcttgtg tatatatggc attctattgg
    97621 tttatctgtc actggtctta ttgcttctat tatttgatga ccacccataa gttgcatatt
    97681 ttttaagtca tgcatatctg ctgcatctga atgtacgtag tctacacctg catccattgc
    97741 ttctttaact acggctgata tatgaccgta atttacatgt gctaatcctg ctgctatttt
    97801 tatgtgtttc atatttgtat acctccatat tttactttaa ttagtattaa atattagttt
    97861 ttgatatttt tttaatattt tttaatccag tattactttt atttagcatt acttttattg
    97921 ctgtatatat ttcttttatg aacttattct gcttttgtta actgtgaact tattttgctt
    97981 tgtaaattaa tattacactg atatattttt tttcacaaca ctttgactac tccttaaaag
    98041 tacctttata ttgtggtaaa ttatagacat taattgtata taaaaagtta gttttttacc
    98101 acattcttat ggcatttatt gtctatacta actcaattga tatagctagt tcatttaata
    98161 tataatcaga ctataaatat aaatttactc gataaaaagt accattcata acaatattaa
    98221 tgaaagggag atgtaaaatg agaaactgta ttattgccca atctggaggc cctacagctg
    98281 ttatcaatgc ttctgcagtt ggtatttatg aaaaaaattt agaaactaaa tactttgata
    98341 aagtatattt tggtatcaat gggattgaag gtatattaaa caaaaatata atagattcag
    98401 actccttaaa caaagataat gtattaaatt taaagcaaac accttcgtct ggtcttggtt
    98461 cttgtagata caaactggct gattataaaa taaattctta tgattatgat agagttataa
    98521 atatactaga tgaatataaa atagatacat tattttatat aggcggaaat gattctatgg
    98581 acacagtgat gaaattttct caatactgca aaaacactac ttgtaaaata aaatttatag
    98641 gtattcctaa aacaatagat aatgatttat taggaactga caactgtcct ggatttggtt
    98701 ctgctgcaaa gcttatttct acactaataa tggaaacata cttagatttt tctatataca
    98761 aaaataatgg tatatttata gtagaaacta tgggtagaga tactggatgg ttagcagctt
    98821 cttcttcact agctagactt aatgatactc cacttgttga tttaatatac cttccagaaa
    98881 ttgatttcta tgaagataag tttatagaag atgttagaaa aatatttaaa gaaaaaaata
    98941 aggcttatat agttgcatca gaaggtataa gggataaata cggcaacttt atatgcgaac
    99001 aaaaaacttc tgctcatgat aaatttggtc attcccaatt aggtggagta agcaactatc
    99061 taaagaatct tatagtatca tcatcaatta caacaagagt aaaaacctta gaacttggtg
    99121 taatacaaag atgtgctatg catttagctt cagatactga tttaaaacaa gcagctgaag
    99181 ttggtgcata tgggatagaa ctatctaaaa aaggatatac aggagttatg gctatcatta
    99241 atagaacatg caataatcct tataagtatg aagttagtca tactgatatt tctaaaatag
    99301 ctaacaagac aaaatatttt cctaaagagt ggataaatga agaatgtaat tatataacta
    99361 atgaagccct aaattatcta tatccactta ttcaaggtaa accagatatc aaatataaaa
    99421 atggactacc ttatttttca attatttact aggaaattat tataatttga tatacactca
    99481 attctatcgc tcttaaatac ctaagtggct gttaaataaa attataaatt gttatatgac
    99541 agtcacttaa tcgtttaatt atttaactgt ttagtttttt aattttttaa tctttttaat
    99601 actaatacaa tttgttgtaa taatatctca cacccatttt cattttggat agattataat
    99661 tagttagaca ataaatattt tgtcatgata ttattatata tagtgataca aatagaaata
    99721 cttttatcat ggctagaaaa ataaagatta cacaaaggat ttaaaagtca aacaacttca
    99781 attaaaataa atggttaaaa taacttctaa atcaactaca taaacaataa aagaacataa
    99841 agtagttaaa tctacaatta taaaattagt aagactataa taacttaaat aaaattaaag
    99901 ttaacaaaga ctattaaagt tagcaaatat tcctctacct aaataattgc tttttataaa
    99961 gagttattta ttttaattga atttaattga aatacgattt taactagaac aatagattaa
   100021 tgtaatattt aatataaaat aattataata attgaaaagg aagtaatcta tgatgaaaat
   100081 agcagtcata acaaccgaat ttttaaaaga gtttgtagat aattctataa aaaagttaaa
   100141 tattaatgca gaaattgaaa tatatatcta tagagatttt tctcatgttg gagatttata
   100201 tttagaaata gaagataggt ttgatggatt tgcagttagt ggccctatcc ccaaaaaagc
   100261 tattacaaaa aaggctggaa ctattaaaaa acctctagtt gattttggta ctgacttaca
   100321 aagttactat gatatgtttc taaagcttat ctttaaatat aatacattag atttttctag
   100381 agcatacttt gacttaacag actgggcaga taatccaaaa gatatatcat attatttatc
   100441 aaatggcaca tttggagact taatggatag tattgatgaa aatgcatcta aaatgagttt
   100501 atctgacata gcaaaacttg aaaaaaagat tgcaaaaaaa catattaatc tatggaatga
   100561 agggaaaatc gatttttcta ctactcgttt tagtagtatt gttcctatgt tacaaaatgc
   100621 aggagtaaac tttcatttca tataccctga tatagattta ttaaaaaata cttttaataa
   100681 cttaataaaa gatattcatt tacatagaat gaagggaaat caaccaagtg taatatatat
   100741 aacagttaaa gatacaaatt atgatgactc tactctagac caagtttata aaatattaaa
   100801 tagtattaaa aaagataaat taaccgattt tataattgaa aaaaatagta attcttttaa
   100861 aattttcact aaatgtgaaa cgattaaatc attaactaaa aattttactg catgttatat
   100921 aaaagaatct attgaaaata gtacaaatat taaagtatgt gttggatatg gaattggtaa
   100981 tgatataaag caagctatgt ctagtgccat aagtgctaat aaggaatcta aaataaatca
   101041 tttaaattac agtttcttag ttaacgaaaa ttatgagaca ataggacctt tactgtgtga
   101101 taagttactt actgtttcaa ataaagtaac tccacatata aatacaattg ccaaaagttc
   101161 taaattgtct actcttacta ttcaaaagat tatatctgtt actgaaattc tacaaagaga
   101221 tgaagttact cctaaggaat tatcttcatg tttaaatgta acagttagaa ctgctaatag
   101281 atttctaagt agtctcttaa agtctggaaa cgctactata ttatatgaaa aacaaaactc
   101341 aacaaaaggt agacctgaaa gggtatacaa attatttata tcagaaaata aatattaaaa
   101401 tccaaaatgc ataaaaatat tttatcatct aatattaagt aaaattatat atgactttct
   101461 aaaaggtata tctttgcatc tttttaagat atacctttta tgatatttaa taatatttta
   101521 tgttattatc tactaaaatt attttctaac cattttactg cacaatgagc aaataaagct
   101581 gaaccataaa ctaatccctc ttcattaaaa attgctttag gattatgcat accaaaatta
   101641 tatccctgac taatacttcc cattccaatc cataaaaata ctcctggaac ccttagtaaa
   101701 atctctgaaa aatcttctga acccattgca gatggaaagt ctattacatt ttcatttcct
   101761 ataacttctt taacataaga tacaatctca tctcctacat ccaaatcatt tataactgca
   101821 ggagtcccca ttttaaattc cactttagca tttcctccaa atgatttagc tattcctgta
   101881 gatatttcaa taattctatt ttttaatttt tctctaacaa attggtcaaa tgttcttaaa
   101941 gttcctgaca tagatgcttt atttggtata gcatttggta cgtttcctgc attaaacata
   102001 ccaatagtta aaactacagg ttctttctga ggtttttctc ttgaaagtat agtgtttaat
   102061 gctatttgta tatgcgacat tatgtttatt gggtccactc caaattcagg gcttgctcca
   102121 tgagttgctt ttccttctac tgttatttca aatatatccg cagatgccat agctggacca
   102181 cgtgaataag ctacttgacc tgtgttaaga aatttaccca ccatactgtg aaatgccata
   102241 gctgcatcga cctgaggatt ttctaatata ccatcatcaa tcattgattt tgcacctctg
   102301 gtagtctctt catctggttc aaatacaaac tttactctac ctttaagtaa gtgtttatta
   102361 tccataagta ttttagctgc tcctaagagc attgctgtgt gattatcgtg tccacaacta
   102421 tgagatgcat ctttattctt acttttaaaa ggtaaatccg tctcctccat aataggaagt
   102481 gcatccatat ctgcccttaa taataaggtc tttccaccat tttcactgtc taaaacagct
   102541 actattccat tattagacgt caatttcgga gaatatccca ttttttctaa agtactggta
   102601 acaaaggtta ctgttttcgg tatattgcaa ccaatttcgg gattttcatg taaatatctt
   102661 ctccatctaa taatttcccc ttctattgac tgcgcttctt taaaaaaatt tttcatattc
   102721 cttcctccta atcagatagt cagcaaaatc tcactttctt ttagtaatga aatgatgctg
   102781 atataaaaat tataatttta tatattaaat ttcatgtctt tttaaggagt tggaatatca
   102841 tctggaatag gacatttata tgttttacca ttcatagatt tatcaaattc ttcttttgcc
   102901 tttttagcta tctctggatt atttaatatt ttagctccaa acatagccat aatctttgat
   102961 gcatatatca tacctttttc tcctatagaa cttcctgcac aagatgtaaa ctgccagcta
   103021 tgacctggtg ctcctagatt cgtacaccct gtaaaaaagt atgctgtcgg aactatatgc
   103081 attacatctc caatatcagt agaaccataa ctattaaagt ttgtaacatt tcctccacca
   103141 taatatagat gagttccttc tggcaaatta tatttactta tcatagcctt atattgttgt
   103201 gggctttgtc tatctaattc agctgcaaaa tccaattcct ctcgagtcca aggctcttgt
   103261 ttaatctcat tcatacattc tgatacaaga tttgctaaaa catgattgtt taaagtatta
   103321 taacaacctc ctaaaaactc tacttcaact tcagtttcag tcatcattgc agctcctttt
   103381 gctaccttta caagtctttc atatgtactt tctacagctt ctctacttaa agctcttgta
   103441 taataccata ctgatgcttt atcaggaact atgttgggtg ctactcctcc atctgtaatt
   103501 gtataatgaa tccttacatc agaagtaaca tgttctctca aataattagc tcccacatta
   103561 gtaagttcca cagcatctag tgcacttctt ccattttgag ggtctgagcc tgcatgcgca
   103621 gtctttccct taaagtgaaa ctttacagag ttaacagctg ttgaagtacc aattgtagct
   103681 tcactaattt tatttggatg aaaatttata gctaagtcta acccttcaaa cgctccacct
   103741 ctagccataa atcctttacc tgttaattgt tcttctcctg gacatgcata aaatactata
   103801 gtcccactta agttattttc tatcatctct cttttaagcc ctactactgc ccctacatgt
   103861 gctgtacata ataaattatg cccacatcca tgaccatatg cacccaattc aaatgcttct
   103921 tgctttgttg aaactttttg atttaaacca ggtaatgcat caaattctcc cataaaacct
   103981 ataactggtt tacctgaacc aaaactagct ctaatagccg tggctaaacc tcctacacct
   104041 acttccacat caaatccttc acttcttaga aagtttgctg tattttcaca tgctttaaat
   104101 tctttaaatg ctttttctgg attttcccat attttcttac ttaaatcatc aagtttgaat
   104161 ctttttttat ctatctcatt tatagatata cttcctatca tttcctattc ctcctataag
   104221 tatatttact atcaaccaaa tataattgga cctattatac tagcaaatgc cactgatgca
   104281 atggttacag taacaaatcc tcctataatc atctttggta ataaataatc tgttgccctt
   104341 tgtttttctt cttcagttcc ttccatagca tcaatacctt cgttggaaat tatctgtgtt
   104401 gcaggataag ctagcataca agttacacca attgcagctg acattcttgg agaatatccc
   104461 aatagttttc ctacaattgc agtaaataat attataaata cagagcctac aacaagtatt
   104521 cctatagttg gaacaatcat cccaaacaat gctgttggag ttaccttagc aagtcctcca
   104581 ggtaacatca acatacaacc aaacattata attccattag attgagattt atctaaaatt
   104641 cctttttcta aaaaacctat tcgtgtaaaa atcaacccta ctaacaaaca tgctatatta
   104701 ggatttaaat aatagtttgc aggggtaaat cctggtatta aagtagccaa tcctacaaaa
   104761 ttaccaatca tagcaacaaa tgaaagcttt gcaagataca ctagagtaga tgatttttta
   104821 cctaaatctt ctttaaaaat tctcatactt ggaagtttaa aattactttt tgactcatct
   104881 ttcaaaaaat ccccattatc tcttttaaat tttaattctt tatttataca atatgtagtt
   104941 atcggaatgc caaagaactt ttgaaatgat aatactagaa ttacaaatgc agcaagttca
   105001 ggtctatttg cagcttctgc aacagttgtt acgataatac cagcaacagt agaacccgct
   105061 attggaggtg ctgctattaa agcatactct ctaccaaata acatactacc cactgttaaa
   105121 cagccaaccc ctattcctac aatacctact aatgcaatta caaccgtttt ccattcatga
   105181 cataagtctt caagatttat aagtgttcct aagttagtta ttaataaagc tataccaaag
   105241 ttgctcattg cagctaatat ccctgatgct cctgtaatgt cttttggtaa aatatttgtc
   105301 caaaatccta ttaaaaatat aatacataca aataacaatg ttgaaattgc accttttgtt
   105361 ttcttagata aaaactcact aaaagcataa atcattacac atacagtaaa gcaagtaagt
   105421 ggattccaaa aattttccat tgttgtttcc tcctaaatat aataattttt tatcaataaa
   105481 acaataatta taaaatgtat attatgcagt tattcttaac tacatatggt atagcttgat
   105541 attttttgat gatattatat gcgtcctaaa ttattcctaa aatatctaaa taaatataac
   105601 tagagttaac tctctaaata ttaactatag ttatcaactt actagatttc tttttgattt
   105661 ttactttcta cataatttta cgagttagaa ttgtacttca actactctct atattagtat
   105721 acatgttgga agacttatat ttatttaaat attagatata gaatacattt ttgcaaacgt
   105781 cttaaattat tcttaaaata tctaaaacga aataataata tacccattaa accaatatta
   105841 tcagaatata cttcacttta agtcttcttt atatagaaat tttatctatt agtgcatccc
   105901 ctcctttgta aattagacta tttctaaacg aatttagata tcttattcct aggaactatt
   105961 actagaaaca aaaattattt attatattaa atcaatgata agccatactt taaaattctt
   106021 tctatacaag tatgatttat catataatga catctatacc tttatcttta tagtagtcct
   106081 aaattattct taaatttaca ataaaaataa tttcactatt tataaaaata cttgttttta
   106141 tattgtcaaa ctattcttaa tatagattat aattttaaat cttttctttg tcaatatatt
   106201 ctgacatttt tccagaaaat ttataaagtt atatagtgag taaatcatac tatttttata
   106261 taatgagtaa attatactat gaatatgtct aaatatttac ttaagtataa acataaaaaa
   106321 tgtgtattct cctttttata gtaattttag ttgccattac ttttaagaag aatacacact
   106381 ttactttaaa gaccctatac tctattttaa atgctttact agacactaat taacattagt
   106441 tcctaacttc tatttatctt attcctaccc tactactata tccgtagaaa aatctaaaac
   106501 ttctccatta gattttatta cttctttgta ccaatcaaat gattttttct tatatctctt
   106561 aaaacttcct ttgccttcat catctaagtc aacatagata aatccatatc tcttactcat
   106621 ctgacctgta gttgcactga ctaaatcaat tggtccccac atcaaatatc caaaacattc
   106681 aacattatcc tcttcaattg cagacttcat ttgagataga tgcttactta aatattcaat
   106741 tctataatca tcatttatag ttccatcttc ttcgatttta tcatatgccc ctaaaccatt
   106801 ttctgtaatt aagataggta attgatactt tctgtaaata tagttcaagg taaatcttaa
   106861 acctataggg tctattgccc acccccactt actttgttcc aaatatggat tttgaatccc
   106921 accaaataaa gatttatcac ctgataaatt ttcttttgga gtagatacac ttgaaaaata
   106981 ataattaagt cctataaaat ctaacttacc ttctttaaat gcttttttgt cttcatcagt
   107041 cacttcaata gttatattat ttttttcata ttcttttaat ttatattgtg gaaacttccc
   107101 attgcacatt gcatcaattt gataataatc acggtcatta tctaaaaatg catttaacac
   107161 attactagga ttacaattgt atggataaat tggatttagt ccaaatacac atccaacttt
   107221 attgttggga ttgatttctc tagccaactt tgcaaccttt acacccgcca aagtcatatt
   107281 ataccctaca tttgccaatg tttgaacctt attttctatt tcactatact taatacctgc
   107341 tatcatataa gtaaaaatat ccgagtgctc tgtttgaggg tcaatatggt tcatctcatt
   107401 aaatgtaacc cagtacttta ccttatcatc aaatcttctt attacagtct cactgtactt
   107461 taaatacaaa tctataagtt ttctgttatt ccatgaacca tatttatgta ctaaattcat
   107521 aggtaattca aaatgatata gtgttacaat aggttctata ttattttcta ttaatgtatc
   107581 tatcagacct tcatagtatt taatccctaa ttcattagga ttttcatcat ctccatttgg
   107641 aaaaattctt gaccaatcta tagatattct taatgcacta aatcccattt ctttaaataa
   107701 agctatatct tctttatatc tattataaaa atctattcca ttatgagatg gataatccaa
   107761 tttttcttct attttatcag taattactct tggcgtttca taagagccct tagttacaaa
   107821 atccataatt gcaggtcctt tgttatcact atcccatgaa ccttcacatt gatgggctgc
   107881 aatacttcct ccccagaaaa atgtatcttt aatattcatg aattttccct cctaaaattt
   107941 atactattac agttaataat tcttctgatt catcatttga tgagattaca tctaaataat
   108001 cgctagtatt tgtaactaca gttattatag tagaatcaaa gccttcctct ataattttat
   108061 cagattcaaa tactgctaaa gcatctcctt ttttaaccct ttgattttgt gttacaagac
   108121 tttcaaagaa ttttccatct aagtttacag tatctatccc aatatgaatt aatatttcta
   108181 atccttcatc agtcactata ccatatgcat gtttagttgg aaatactata gttatagttc
   108241 catctacagg agatataaca gttgattttg attcactagg tataactcca acccctttac
   108301 ctaatgctcc agatgaaaat gctttgtcat taacttcccc aagtgcaatt acctgacctt
   108361 ttgcaacatt tccaatacta atagttcttg aattgttctc gttgattatt agttctaatt
   108421 ctttttcatt atcaccctta acttcttctt tttcatctga aggtattcct agtaaatagg
   108481 aagctgctgt tgctgctaca aatgatacta ctacacttat tatcataaat gtaaagttag
   108541 atacctctcc tccaacaaga tatgttggta atgcagctaa tccccaaacc attccatatg
   108601 ttttaacttg agctaaacct gctattaaac cacctatacc tccacctatc attactgcta
   108661 caaatggtct tctatatttc ataaacacac caaacactga aggttctgta actcccataa
   108721 gtgcactaaa acttactgta ccaaataatt gtttttgttc tttattccta gtttttaaga
   108781 aatatccaaa cattgcacca caaactgcaa tatcagatat tgttgcagcg cccataaaaa
   108841 ttgggtcata ccccaatgtt gtcacagaat ttaaagatat aggcatagta aagtttgctg
   108901 caccaaagac tattaagagt ggttgtaaag ctgcatacag tgcaacaaca ctccaattac
   108961 ccaatgtatc aattaaaaac atacatgcat agtttaatcc atcaccaatc cacataccaa
   109021 taggtccaaa tacagttaat gtaattgata gagttattat taaagataat actggaacta
   109081 aaaaatagtg taatgcttta ggtaatattt ttttcaaacc ttcaattata attcccattg
   109141 cccatactcc taaaagtatt ggaataaaac tgtttgcata agttgtagcc tgtaaattta
   109201 taccaaataa acttaatcct tctacaccat ctattgatga agataataac gtaactgcta
   109261 acactactgc tatataggga tttacattaa gtctttttgc tgatgctatt gcaattaata
   109321 ctggtaagaa gtgaaatgta gcacttctta atgtatctaa tatttgatat gtaggactgt
   109381 cactctgaag tatgccagac atatttaaaa gtgctaacat acttgcaagt ataccagctg
   109441 caagtagtac ttcaattact gaagtcatag tctctgagac taccacaaag atggaattaa
   109501 atataccttt gatacctcta attccctttt ttctttcact ttttatactt gattcacttg
   109561 aacttaaacc tatcatagac ataaactcgt tatatacatc tacaacatga gtacctatta
   109621 taatttgata agcaacatca ttagaaacta catcaattac tccttttaat tttttaattt
   109681 cttctgtttg agcgatactc ctatcattta aagtaaatct taaacgagta acacaatgcg
   109741 ctaccgactt tatattttct tttcccccta taagttttat tatattttca tttaaatctt
   109801 tatatttcat ctgattttgc ctccagtctt tgagtcaccc tatttatatg caacattaaa
   109861 taagtttctt cacttataga tatatcgaca ttaaattgtg attttatata ttttcttatc
   109921 ttttgaacac ttccaaatga ctttggataa agttgactta cttgtttata taaatctata
   109981 gaattatctt catctattga agcttcatcc gacataattc tttggataaa gtactgtaga
   110041 tgagtagtaa atctcatata atttgttgaa gtttcatcta gctctacatt aaaatgatat
   110101 tttattatag aaactatatc tgctaaagtt ctcaactcta caattgtact atcatgagac
   110161 tctttattta cctccatatt tacaaaatga agtgctattg atactgcctc atcatcagga
   110221 agattaagtc ccttactatc accaattaat tttagtgcat gcattccaat ttcaaaatgc
   110281 tttggataaa atcttttaac attccatacc aaaggacttt ttataaattg attcttttca
   110341 catcttttaa gtaaaaactc taaatggtct agtaaggtta agcttagata atcacttgac
   110401 tttatattta aagctttctc accatattca attatttcat taataagaac tatattattg
   110461 gagtctgagt tttgtagtag ataactaaag tgttctagca tatcgtatga atctagtata
   110521 tatgttctct ctatttcatt ttcatttatt acttgtccca ctcttttttt aaagccaatt
   110581 cctttaacaa atacaataat ttcattttta cctcttttta ctaaggctac attattattt
   110641 aaaatctgaa ttattttcat atattcctcc cttcaataaa aagaactcaa gcatatatgt
   110701 atagagtatc cctctatttt tcacatatat acttgagttc tgcctgctaa tcagtaacat
   110761 actcaaaatt atgactacgt cgccataatt aaaattcttt tttgtttaat ttcatattct
   110821 atatgttata ctatccatat aaatctgtca acggtttcct agatattttt ttattctata
   110881 actacaagct ctgttatgcg attatctgac aaggtttata tactaaattg atactatttt
   110941 accgtcacta atatttataa tattgtctga accattatct attttactat tatgaattat
   111001 catagtaatg cttttaaata taatgaactg tatgttaaca aataaattct taagatatat
   111061 taacatataa agtcttaagt ttagttttat ttaatcacta ttaagatata ttaataggta
   111121 aagcttcaaa tttagtttca tttaaatgct agtaagatat actaatatat aaat
//
LOCUS       DAGSRM010000003        99531 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.003, whole genome shotgun sequence.
ACCESSION   DAGSRM010000003 DAGSRM010000000
VERSION     DAGSRM010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 99531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 99531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..99531
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.003"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            227..610
                     /locus_tag="MJE33_000188"
     CDS             227..610
                     /locus_tag="MJE33_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646171.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKMVLKSPEYIGSHPKKPGKSVEFIKKIDDYILVATELDTKKGYLYV
                     SSMYAIKEAKLESRVNSGRVKQYDG"
     gene            1116..1499
                     /locus_tag="MJE33_000189"
     CDS             1116..1499
                     /locus_tag="MJE33_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBY9646172.1"
                     /translation="MNLTKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGVLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLEDKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
     gene            1501..1851
                     /locus_tag="MJE33_000190"
     CDS             1501..1851
                     /locus_tag="MJE33_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646173.1"
                     /translation="MDNTITINISIIYVLLAILACIALVYLIIVLVKLHKILNNVGTM
                     LNENTQNINTTLNNLPNVVETFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFLDKK"
     gene            complement(1952..2410)
                     /locus_tag="MJE33_000191"
     CDS             complement(1952..2410)
                     /locus_tag="MJE33_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646174.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSSGRADFLYSSRLQKLLNEVYINQSRASYISGCGLFWSSYWDILEEKFEEWLY
                     NSYIFFDEDDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(2455..3243)
                     /locus_tag="MJE33_000192"
     CDS             complement(2455..3243)
                     /locus_tag="MJE33_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBY9646175.1"
                     /translation="MKVVIIPGHTLIGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAVQIHFNSNATTSTPVGTETIYKTNNGKTY
                     AERVNTRLATVFKDRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQGGGENKVYENVIVYTGDADKVAAQILHWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALQEIDKL"
     gene            complement(3382..3819)
                     /locus_tag="MJE33_000193"
     CDS             complement(3382..3819)
                     /locus_tag="MJE33_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HBY9646176.1"
                     /translation="MNKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKNCSIYVTHF
                     PCLNCTKAIIQAGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
     gene            4641..5150
                     /locus_tag="MJE33_000194"
     CDS             4641..5150
                     /locus_tag="MJE33_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY9646177.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGLSVPDSD
                     MLISISEVLETPVSTLLGETIVESEVDSLKVISEKLEIINLQLAQRKNMRRKMFHWLF
                     ILLCAVILIGFVVLGGLNSPYLDWNYTDPETAVFGVAFHAFEWLFVRLVPIVLIGAII
                     GIFVTRKKI"
     gene            5555..6091
                     /locus_tag="MJE33_000195"
     CDS             5555..6091
                     /locus_tag="MJE33_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBY9646178.1"
                     /translation="MQGGVNIRQVKSFVFPVISLIFLDQISKVLIGLFLMDFEIDIIG
                     KFLRFNPVQNTNLSYGGNFIGILSNLWVLVLFNILVILVIISGYAFYKSKNEQTSYSV
                     KVIMSCGLAGTICSLIDKLFWGGSLDFLQIPSFFIFDLKDCYLTVAEIIFVVIGILHN
                     REISMKEYIYFCYRQFKK"
     gene            complement(6525..7325)
                     /locus_tag="MJE33_000196"
     CDS             complement(6525..7325)
                     /locus_tag="MJE33_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9646179.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYAFSGQLFKHIGISVYETVLGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGATKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
     gene            complement(7520..9118)
                     /locus_tag="MJE33_000197"
     CDS             complement(7520..9118)
                     /locus_tag="MJE33_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBY9646180.1"
                     /translation="MKAAIYSRKSRFTGKGDSIENQIQLCIDYAKSIGVKDFLIYEDE
                     GFSGSNIDRPQFKKMMQDAKDKKFSYLVCYRLDRISRNVSDFSTLIEKLNELNISFIS
                     IKEQFDTSTPMGRAMMFISSVFAQLERETIAERIKDNMYELARSGRWLGGNTPHGFNS
                     EKITFLDENLKERSMYKLQVNDEQMEIVKLIYNKYLELRSLSKLYKYVYNNGIRGPRG
                     GKFDPSSLSRILRNPAYVKANDEILDYLKNSGMDVVGTPDRKCGILTYAKNTSNSIAA
                     IAKHHGVIEPEIWLEVQNQLDKNRDKTPRISTGKTALLSGLIKCGKCGSNMRITYKGK
                     KTDKDLKYYYICGTKKTLGSDSCNCKNLNGPLVESLVIDKIKNCKEESIISAFNSNNI
                     KTDNVFNIFKSETNLLKNQILDKERLIKNLVIELAKNTGSTASDYIVSQIESLNNEIS
                     ILKDKINILESSNINISSDSINIDIIVSNLKRFNDEVDNASLDDKRLLLSTIINSIVW
                     DSSLGNLNIVYMGAEVDNSVSLSN"
     gene            9446..9622
                     /locus_tag="MJE33_000198"
     CDS             9446..9622
                     /locus_tag="MJE33_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646181.1"
                     /translation="MEINVIEIFPKDKAKLNKIEMDKASWFVNIISKKYPKEALNEAF
                     STLEKELNISKANT"
     gene            9796..10926
                     /locus_tag="MJE33_000199"
     CDS             9796..10926
                     /locus_tag="MJE33_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBY9646182.1"
                     /translation="MNNFEIKTRIKFGEGSLKALQELKNKNVLIITDPFMVKSKTIDK
                     ITSNLINSTYQVFEEIVPDPPIEVVVAGIEAFKSINPDVIIALGGGSAIDAAKSIMDF
                     SKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
                     VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKAIKLVFKYLIKSYKDGNDI
                     EAREKMHNASCLAGMAFNQASLGINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSI
                     VGYTNTTYSDTAKKYAQIAELVGLGSANIRISVRNLVNEIKKLQKEMNMPTKLTQCKL
                     SLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILKNIK"
     gene            11167..11517
                     /locus_tag="MJE33_000200"
     CDS             11167..11517
                     /locus_tag="MJE33_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBY9646183.1"
                     /translation="MTEESKQRVIQEYVPGKQVTLAHIIANPNEDIYKKLGLVLDKKD
                     AIGILTITPSEASIIAADVATKASNVSLGFIDRFSGSVVISGDVSSVESALNDVLEVL
                     GNMLNFSSTKITRT"
     gene            11525..11974
                     /locus_tag="MJE33_000201"
     CDS             11525..11974
                     /locus_tag="MJE33_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HBY9646184.1"
                     /translation="MKNIIFMGKTGSGKTTLCQKLDQLELKYKKTQSVELYNNAIDTP
                     GEYMENRNLYNALITTSADAKIIAIVYDATQEENYIAPGFASMFCKDVIGIITKINKA
                     TENEVNIAYERLSLAGASQIFKIDTVDNIGLEKLFKYLEKYKNIDEV"
     gene            12076..12651
                     /locus_tag="MJE33_000202"
     CDS             12076..12651
                     /locus_tag="MJE33_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBY9646185.1"
                     /translation="MGKKILLVDDEPLLRMDTKDILESHGYEIIGEASDGFEAVEMCK
                     KHKPDLVIMDIEMPILDGIKAGKIICKENLAGGVLLLTSFEDKEYVEKAKSIGAYGYL
                     VKTASEKSLIPTIEMCLNKVNEFEKMKKDLEKVNSKLNERKLVERAKGILIREFKISE
                     DEAYTRIRKLSMDKRTTMMEIAKMIIIGYDD"
     gene            12644..14053
                     /locus_tag="MJE33_000203"
     CDS             12644..14053
                     /locus_tag="MJE33_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HBY9646186.1"
                     /translation="MTNTIKKISTAQTYLNNNEIEKIIEVSKSLDLMASFYEADVFID
                     VLSKFDDEAIVVAHGRPKEKSMYCENVIGKKALIENEPGVIKCLKTGERVRDIKALTQ
                     EYKLVKQNIQPITLDDKVLAVLIVEKDISSEVKNEFNASEDEENKNLHQFIKLIGNND
                     FLVDNLNSAILIFDKNGKLKIKNRIATEIYKNLGFGYDIQDMHYNDLDIETNTFENIL
                     MDENYDETNEVSIGNMFFKIKTIYIKDDEFKVCKIVQDISDLKRKEEELVLKTVAIRE
                     AHHRVKNNLHTVISIIKKQSRLSQNDEVKQCLDNIINRVFAILSTHYLLSKEVDNNIS
                     IREGINLLVSNIQGGYLFSKDIDIRITGEDFKISGEKATAILLVINEILQNCYDHAFS
                     DREHGTIQILINKDNDYRNIAIIDDGIGFDSNNINKNSLGTYIIDSYITQMLKGNIER
                     KSSENGTEVLITIPTQINI"
     gene            14237..15670
                     /gene="eutA"
                     /locus_tag="MJE33_000204"
     CDS             14237..15670
                     /gene="eutA"
                     /locus_tag="MJE33_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HBY9646187.1"
                     /translation="MREELLSVGIDIGTSTTQLIFSKLIVENTESNFSVPRISIVDKE
                     IIYKSKVYFTPLISNNEINGQQIKDIVENEYKKANISKEDIHTGAVIITGETARKENA
                     NDVLHTLSGFAGDFVVATAGPDLESIISGKGAGAHIYSKEHSTTIVNIDIGGGTSNIA
                     SFKRGEVTDTGCLDIGGRLVKIDKISKKITYISPKIDKIIKDNNLNLQVGTMATVENT
                     KPIVDIMVDLLMESIGLKKQTPLFDFIITNKSISIDETIKNVSFSGGVADYIYYDGEI
                     KDYFEYGDLGILLGQAIKNCEYLKKVNVVKSMETIRATVVGAGSHTTEVSGSTITYTS
                     EKFPLKNLPVLKLSIDEETGTSKQISEAIKNKLNWFKLENDLQKVAIAMIGKKNPTFL
                     DIQEYAKGFVDGMQQIIENDLPFIVIVENDMAKVLGQAIYALLNYEKEIVCIDSVKVE
                     NGDYIDIGKPIVEGKVLPVVIKTLVFN"
     gene            15688..17052
                     /locus_tag="MJE33_000205"
     CDS             15688..17052
                     /locus_tag="MJE33_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HBY9646188.1"
                     /translation="MILNTKLFGRTYQFKGLYDVMAKANEEKSGDKLAGLAAESAEER
                     IAAKVVLSHIKLEDIRNNPAVAYEEDEVTRIIQDAVNEKVYEEIKNWTVSELREWLLD
                     SNTTSLDIRRISRGLTSEMIAAVAKLMSNMDLVYAAKKIKVTAHCNTTIGEQGTLSAR
                     LQPNHPTDDPDGILASLLEGLTFGIGDAVLGLNPVDDSVESVTKVLKRFEEVKQKYQI
                     PTQTCVLAHVTTQMEAIRNGAPADLIFQSIAGSEKGNEAFGFNGDTIEEARQLALKHG
                     TATGPNVMYFETGQGSELSSEAHHGIDQVTMEARCYGFAKKYEPFLVNTVVGFIGPEY
                     LYDAREVVRAGLEDHFMGKLHGLPMGVDVCYTNHMKADQNDMENLAILLTTAGCTYFM
                     GIPHGDDVMLNYQTTGYHETASLREMFGLTAIKEFDAWMEKMGFSKDGKLTELAGDAS
                     VLLG"
     gene            17065..17946
                     /gene="eutC"
                     /locus_tag="MJE33_000206"
     CDS             17065..17946
                     /gene="eutC"
                     /locus_tag="MJE33_000206"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014952556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HBY9646189.1"
                     /translation="MNEKDLKALVEQLVGQMVGELDTNVVSETVKKATEVVVDNNAYI
                     DDITEVDIRKQLLVKNPKDAEAYLDMKAKTPARLGIGRAGTRYKTETVLRFRADHAAA
                     QDAVFSYVDEEFIKGNNMFAVETLCKDKDEYLTRPDLGRKFSPETINNIKSKFGTNQK
                     VLILVGDGLSSAAIEANLKDCVPAIKQGLKMYGIDSSEILFVKHCRVGAMDHLGEELG
                     CEVICMLVGERPGLVTAESMSAYIAYKPYIGMAEAKRTVISNIHKGGTTAVEAGAHIA
                     ELIKTMLDKKASGIDLK"
     gene            17966..18619
                     /gene="eutL"
                     /locus_tag="MJE33_000207"
     CDS             17966..18619
                     /gene="eutL"
                     /locus_tag="MJE33_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HBY9646190.1"
                     /translation="MKNDVIRPNILGIKIISNISPEMAQKLELKSHHKSLGLITADCD
                     DVTYTALDEATKASEVDVVYAKSMYAGAANASTKLAGEVLGIIAGPSPAEVRSGLNAV
                     VDFLEYGATFISANDDDSIAYYAHCVSRTGTYLSEVAGIREGEALAYLVAPPLEAMYA
                     LDAAMKAADVKMCELFAPPTETNFGGALLTGSQSACKAACDAFAEAVKSVADNPTGF"
     gene            18630..19331
                     /locus_tag="MJE33_000208"
     CDS             18630..19331
                     /locus_tag="MJE33_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBY9646191.1"
                     /translation="MDAIGLIEVIGYVAAIEASDACLKSANVNIVRVDKVGAGIVTLT
                     ICGDVGAVKAALEAGEIAASRVGTLRTSHIIPRIHNEVTNALFKTKEPKVCEVSEDKE
                     KNFEITLEITDNVSKNLSEKENVILDVNENSGNTENLYSDIDKTNKKIETVDDSLTEA
                     NEEKIENTNDKEVLEQVKIENSDFDRDDLSKNSVKKLKAMAKKLDSSITYKDLNVLKK
                     EELINLVKKLSRRDR"
     gene            19334..20803
                     /locus_tag="MJE33_000209"
     CDS             19334..20803
                     /locus_tag="MJE33_000209"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HBY9646192.1"
                     /translation="MTLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKSVV
                     DAAFNNATKLAKMANEETGFGKWEDKVIKNTFASMGVYETLKNTKTIGIIGEDKEANT
                     IDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISE
                     AAIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVG
                     PGNGPAFIDKSANVKLAIKRIIDSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGA
                     YVLNEEESERLAKFILRANGTMNPQIVGKSVEVIANLADLTNVPKNTKVLVARENKVG
                     HNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHVNDEELVKRFALN
                     MPVSRILVNTPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGI
                     KEIEELGRDKSQVEKSMFDSMDKEELINLLVQKLVKELA"
     gene            20899..21186
                     /gene="eutM"
                     /locus_tag="MJE33_000210"
     CDS             20899..21186
                     /gene="eutM"
                     /locus_tag="MJE33_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HBY9646193.1"
                     /translation="MASANALGMIETKGLVGAIEAADAMVKAANVQLVGKEQVGGGLV
                     TVMVRGDVGAVKAATDAGAAAAERVGELISVHVIPRPHFEVDAILPKVSAE"
     gene            21313..22074
                     /locus_tag="MJE33_000211"
     CDS             21313..22074
                     /locus_tag="MJE33_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP--cob(I)alamin adenosyltransferase"
                     /protein_id="HBY9646194.1"
                     /translation="MGVLTESEVKRKIGKNKNNITEFYIEKGQIITPSAKSYLAERSI
                     ELKYVDKLSDVNNEPKEVVKEVVKEKKYRYTTVFGAKLDEKPEHMTQLNGNLLVFKDH
                     KRIILRGKIDSLESEILKVQILCQKLDMPKLVKELQEVLDFVRNIMRCEVLDEKLGDF
                     KLLGLTPQELREQSHYPKKYFGIGHEFPSYEMGEVVVAINSIRSNTREVELAAYEAFK
                     GEYGNVHREDLIKALNRLSSVFWIMIYKVRTNKYK"
     gene            22087..22716
                     /locus_tag="MJE33_000212"
     CDS             22087..22716
                     /locus_tag="MJE33_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBY9646195.1"
                     /translation="MENLLNEIVEEVITRVKKEAFIEVEASGRHVHLSREDVDKLFGE
                     GYTLTKLKDLSQPGQYACKERVTITGPKGSIKNVVVLGPCRNETQVEISLTDGSTLGL
                     KAPIKQSGDLEGTLSIKISTQYGEVELDKGLMVAKRHIHMTPKDAEKFNVCDKEIVEA
                     KVMGQRPLTFDDVVIRVSDSFKTYMHIDYDEANACGYSKGTVAKIIKKA"
     gene            22758..23483
                     /locus_tag="MJE33_000213"
     CDS             22758..23483
                     /locus_tag="MJE33_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02536 family ethanolamine utilization
                     protein"
                     /protein_id="HBY9646196.1"
                     /translation="MNYDNLVDLITEEIYKKINSNEIKISNKPKAVIVFEQDNNKFNL
                     LKDEFEIVEFDKSIRECEIVIVSRLCMRGLSNIALGNSTSDEERFILKMIMKGKEVYV
                     LDEGIEYKKYKDTAPKALYKKFMSFEDEICKFGVEIIKDLNVITKNKLNIKKELESTR
                     GSKKEDTKVLDKDVLNLISVESVKIDNEFSLDLRNKKLISEADLRKPTINGVKNILVN
                     KKSIITPLAVDFMRIHHLKLKKV"
     gene            23496..23768
                     /locus_tag="MJE33_000214"
     CDS             23496..23768
                     /locus_tag="MJE33_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HBY9646197.1"
                     /translation="MDIGKVVGNVWATRKDEKLCGQKLLVVKILKTKDTYKEGFMVAA
                     DNAGAGNGDLVLISKGSSARESIEKSHVPVDATIVGVVDSFEVFDE"
     gene            23761..24312
                     /locus_tag="MJE33_000215"
     CDS             23761..24312
                     /locus_tag="MJE33_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBY9646198.1"
                     /translation="MNKSIGAIEFKSIAKGIEVSNEMIKKSSVDVLYLKSICPGKFLI
                     IVGGETSYINECVDYGIKLGEGYIVDNFVINAISQEILDGFKNKYQKLDSIVSIGVVE
                     SSKVCAGIKMLDKTLKSGDLVLVKLQLSFAIGGKLVYIVAGDLSSLEYALKESENVVR
                     EKEVIYKTVIPSVDSQIIKSLIK"
     gene            24324..25415
                     /gene="eutH"
                     /locus_tag="MJE33_000216"
     CDS             24324..25415
                     /gene="eutH"
                     /locus_tag="MJE33_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBY9646199.1"
                     /translation="MSINEIIIYVMVVFMVLGAIDKCIGNKFGLGEQFEEGIMAMGSL
                     AVAMVGVICLAPVLADVLRPIVVPVFNILGADPAMFAGSLLANDMGGAPLALELAKDP
                     NAGLFGGLIVGAMMGPTIVFIIPVALGIIEKEDQKFLATGILAGIITIPIGAFVGGLV
                     AGFEVMMVLRNLVPIIIFAVVIALGLLKFENAMIKGFTYFGKGVVIVITLGLAAAIVE
                     ALTGIVVIPGMKPISEGIEIVGDIAIVLAGAFPLVFVITKVFNKPLMALGKVLGMNDV
                     SAAGLVASLANCIPMFGMMKDMDNRGKIINVAFSVSAAFVFGDHLGFTAGFNSNMITP
                     MIVAKLVGGITAVLLAMVIANKTLKKENV"
     gene            25417..25890
                     /locus_tag="MJE33_000217"
     CDS             25417..25890
                     /locus_tag="MJE33_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF861 domain-containing protein"
                     /protein_id="HBY9646200.1"
                     /translation="MDISNIDKNLIETLVRQIIEEKISGTKDTVDFVRNKDISGITSI
                     KLPTVKVSESDRLDTGNPSDVVYTKDLFTLEESPRLGCGMMEMKETTFDWTLNYDEID
                     YVIDGTLDIIIDGRKVSASSGELIFIPKGSKIQFSVPDYARFIYVTYPADWASQN"
     gene            complement(26010..26111)
                     /locus_tag="MJE33_000218"
                     /pseudo
     CDS             complement(26010..26111)
                     /locus_tag="MJE33_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            26406..26927
                     /locus_tag="MJE33_000219"
     CDS             26406..26927
                     /locus_tag="MJE33_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBY9646201.1"
                     /translation="MEEIVKKEQASMNDIFLYLKAKSNSLYKFVILYNEFINKPKDYG
                     TGELINMVEVHMLTAIEENPGISPTQLAKIWNRTKGAISQTISKLEKKGYITKEKKEG
                     NAKTILLYPTTSGVELSKAHKLYDTIDVTRTFEYLIKECTLEEIDSFYKVIDLFINLL
                     EESPQENNDSEKV"
     gene            27306..28481
                     /locus_tag="MJE33_000220"
     CDS             27306..28481
                     /locus_tag="MJE33_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBY9646202.1"
                     /translation="MNLKQLSEKYEEYVIQMRREFHKYPELSFKEIRTTQRIREELQK
                     IGVPFVSIEPNIVIATIQGKNEGKTIAIRGDIDALPMQEDSDVEYKSEYDGIMHSCGH
                     DAHGAMLLGLAHMLNDIRKDLKGRVLLVFQAAEEVGGGHKEIIQYFKENGGPDRLIAT
                     HVWSDIEAGKISVEPGPRMAGGSGWRIDITGRGGHGSRPDQSIDPIKPACDIVLKVSS
                     IPVNHISVLEQCVVHACAIHGGTTIGNVIPNNAEVIGGYRYFTEESGKKVFDIIKQMS
                     NGVGQVYGVDVKVTHTGGIGPVINDEEAVKMAKEIVSNIDGLELDSYEPICASDCYGE
                     ILKEYSGFYCYLGVGNKEKGIIYAQHHPKYNIDEDTLKLGCEFMAKYAVEFLNKNNN"
     gene            28516..30054
                     /locus_tag="MJE33_000221"
     CDS             28516..30054
                     /locus_tag="MJE33_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBY9646203.1"
                     /translation="MQGALKENKKKSGGGFLGWIEKVGNKMPHPLALFTYITLIVVAL
                     SAIASFLGFSATSPATGKLIKVMNLISAKGLVLFMQEFVKNFQNFPVLGVVLVMAVAT
                     GVCEKTGFFSTAIKMSLSKVKGNAVVFIIAFIGVFANQAGDAAFVLVPTLAGAIFYGL
                     NRNPLAGIFCGFASVGGGFATAVIPGGWDVTLTPITVSAAQTIQPAFDMTLLASYYAL
                     FVSAFIVATVSTIVTVKFIEPKLGKYTGKPEGIDDNQGFEVTKEQAKAAKLAGFTVLA
                     YVALLIALCIPANSFLRSPEGSLIFGAPLMSCLQFFIVILFFLPGIVYGMRVGKIKTV
                     KDLNDILCQSMAAMAPFIVLAIVIGQFLTLFSVSNLAQVLAIKGGALLNSLPLPPQAI
                     ILLFLVLVAFINIFVISGSTKWMIFGPVFVPMLMQLNIHPAFTQFVYRLGDSVTNHLS
                     PLNAFFIILLATVQKYDKNAGMGTIFSAMIPYTIGYFVVLAVLVVVWMTIGIPVGIGG
                     QVWL"
     gene            30086..31288
                     /locus_tag="MJE33_000222"
     CDS             30086..31288
                     /locus_tag="MJE33_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBY9646204.1"
                     /translation="METTEIRYNFDEVIDRSGTNCLKYDLKSQFMPESPEDALPLWVA
                     DMDFACAPEIINAMHERIDRKIFGYSSQQTDDYYEAVCGWFKRRFDWKIEKENIFFSP
                     GVVPALGFFTQILTQKGDGIIIEKPVYYPFEDKITNNERKVVNSPLKFEDGHYSMDYD
                     GFEELAKDENNKVFILCSPHNPVGRVWKKEELRKIVDICKKYDLWIISDEIHCDIIRK
                     GEKHIPLEVACPDYKDRIITCTAPSKSFNLAGMQLSNIVINNKELQKKWMLETDSKLA
                     IFLPNPFAIVATKVAYNDCEDWMNQVNEYIDGNIKYVEDFVNEYMPKVKIIKSQGTYL
                     VWLDFRGYGLDAKQLEYVMLNKAKVLLDEGYIFGEEGAGFERINVASPRTIIEECMNR
                     IKCAFEGL"
     gene            complement(31455..32054)
                     /locus_tag="MJE33_000223"
     CDS             complement(31455..32054)
                     /locus_tag="MJE33_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBY9646205.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVTHDIGEARCIKVQL"
     gene            32224..33582
                     /locus_tag="MJE33_000224"
     CDS             32224..33582
                     /locus_tag="MJE33_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646206.1"
                     /translation="MSNIITNLIESIFTNPITILFLLAITVYSVFKIIENNRVYSFVS
                     SKLDEINRDYKTSEFYKKVRDDYYTYSKENPYADVNITSFIEEVVSDLKHNNLPLLEK
                     IRSIKNSSSISILLGVLGTFVGLSTMLLCVDTKDIINSLPSTISSMQTAFTTSIFGVV
                     FSLIIGYFTKIKDCEHVLIQIMLKSENLLTSEITHFKSERMDLKVEEVKNTIKQISKS
                     IEAIEKFDKISKDLNDFNDEFISGIEALKSLLEGSQSSIKTFDQSVRKLDKQFNILNI
                     KFVKLFDKYDNQDNINKEILFDIKESSKNIYNATESQFKIRDYIKNINAGFALYERSA
                     QDLLTKLMTHENKISQNQKILLDEKFTLDDSIKNLSSIIENFSNDLQVKLDMMFENSL
                     DIQDKLDVMFNNSFMNDEVPLDSEELFKDDVNNVFNPFSEEIYEIEDKEIKVIGEDEL
                     NE"
     gene            33575..34207
                     /locus_tag="MJE33_000225"
     CDS             33575..34207
                     /locus_tag="MJE33_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBY9646207.1"
                     /translation="MNNKYRRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNASVEETFKKSGIPVKVDKSNGQVTFGEKTMFDVDSDVLKPEAKEML
                     KMFVPKYIETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNGDNSVNRDASRRVELKYEININQNK"
     gene            34536..35693
                     /locus_tag="MJE33_000226"
     CDS             34536..35693
                     /locus_tag="MJE33_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBY9646208.1"
                     /translation="MIEISIKNKKNSKIKTLILLLIVSISIYLTGCTTSDNGNNQSNN
                     NDSLSSSISSDKNVKIHFIDTGNSDAILIQDGKTFTLIDGGDNDDEGLMVDYLNNQGV
                     KDIKYLIATHSHADHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALANKGLSPS
                     VPLENNKFYLEDSYFEVLNTNGGDTTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILP
                     KLEKVDLLKVAHHGSRSSSSQEFLDKVNPEYAVLLVGKGNSYGHPHQETMNKLKKMGV
                     KVHRSDECSDIIFESTGDGVFTSCKDGSYNKGVREDGNSGKSNSNTSKNDSFKNKQNT
                     SNSEEIVYFTPKGKSYHSTKNCSGLSRSKKILSGTIAESKKNDPCDICYGK"
     gene            complement(35840..36475)
                     /gene="lexA"
                     /locus_tag="MJE33_000227"
     CDS             complement(35840..36475)
                     /gene="lexA"
                     /locus_tag="MJE33_000227"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBY9646209.1"
                     /translation="MYLDLTEKQVLILEFIKSQIILKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLRVKGESMINAGILDEDYVVVDKKNTALNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(36579..37706)
                     /locus_tag="MJE33_000228"
     CDS             complement(36579..37706)
                     /locus_tag="MJE33_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBY9646210.1"
                     /translation="MNNDKKIIKVHNKSDKPDNIVIKENELIEKCLLLENKLPVFMKD
                     YFIYLKGSVAVSTRLAYLEDINFFCSYLIETKELTNADCVKDITEDDFNTIKSRDINL
                     FLGDYCSRYYKNTEKNTLIFENNNRALARKKSSISTLFKFLYRNSQIDNNITDGFNPI
                     KLPKPQPDAIKRLEIDEVAKMLESVETGEGLTEKEKVYWRKTKLRDKAILALFVTYGL
                     RLNELRELNISSFNFSRGEFKIYRKRGKEVLMPINHTCEHVIKDYLQNERTRDELLND
                     EEKDALFLSLQNKRITAKAIRTLVKKYTSIPLDTTRDNGYSPHKLRATAATSLIQTGF
                     SIYDVQNLLDHDNVTTTQLYAAHKKNVKRDIVKNFEWIEDD"
     gene            complement(37990..38835)
                     /locus_tag="MJE33_000229"
     CDS             complement(37990..38835)
                     /locus_tag="MJE33_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646211.1"
                     /translation="MIYIIEILKISVINIFCFIGIFIVFGLIFSITENLNNKFIFSSF
                     GKTGIIVTGAIGTFVHELSHLIMCLIFMHKINSVKFFRPIESKNDGILGYVSHSYKKD
                     NLYQSIGNFFIGIAPIIGGTIMIILIFKILLPDSYMKVTQNIDLKLYVSMINNLNING
                     FIHIILGDISNFIRIMLLTPSVYSLKYFIFMFLMYSISTHMSLSSADLKNSLNGLSFI
                     VIVIFVISSITYLFGFDNLNISSLIVKYNIFISFFMAIGFIFSIVTLIISYILSLVSP
                     FKNFS"
     gene            complement(38865..39236)
                     /locus_tag="MJE33_000230"
     CDS             complement(38865..39236)
                     /locus_tag="MJE33_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBY9646212.1"
                     /translation="MKLKLLVEEYAVCRLNNDSKIPTWIDTEKFYSITRTDDELSVVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIENACKILSCNGYEVE"
     gene            complement(39389..39649)
                     /locus_tag="MJE33_000231"
     CDS             complement(39389..39649)
                     /locus_tag="MJE33_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HBY9646213.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGVAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            complement(39838..40782)
                     /locus_tag="MJE33_000232"
     CDS             complement(39838..40782)
                     /locus_tag="MJE33_000232"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HBY9646214.1"
                     /translation="MIENNSEKIKILENDIKQLITISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSAYEKVTLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSMVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYKKFRNIGKCL"
     gene            complement(40803..41657)
                     /locus_tag="MJE33_000233"
     CDS             complement(40803..41657)
                     /locus_tag="MJE33_000233"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBY9646215.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVVTGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKSLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKEVLY
                     QILAMHKK"
     gene            complement(41672..43033)
                     /gene="accC"
                     /locus_tag="MJE33_000234"
     CDS             complement(41672..43033)
                     /gene="accC"
                     /locus_tag="MJE33_000234"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBY9646216.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEDNNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            complement(43049..43501)
                     /gene="accB"
                     /locus_tag="MJE33_000235"
     CDS             complement(43049..43501)
                     /gene="accB"
                     /locus_tag="MJE33_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBY9646217.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQSTSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADSFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
     gene            complement(43771..44217)
                     /locus_tag="MJE33_000236"
     CDS             complement(43771..44217)
                     /locus_tag="MJE33_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646218.1"
                     /translation="MESKFFNWYTQSLGGTFGIISCICAYLNGYMFVYTNSASKYFES
                     IGFLGIISSYTLLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILITVVFGF
                     IGARVYFIIPAILITFNLYSNVLIAKLKYILCKNKENDTNLKESEV"
     gene            44492..44770
                     /locus_tag="MJE33_000237"
     CDS             44492..44770
                     /locus_tag="MJE33_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646219.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     VLVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
     gene            complement(44899..45645)
                     /locus_tag="MJE33_000238"
     CDS             complement(44899..45645)
                     /locus_tag="MJE33_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646220.1"
                     /translation="MWEGTMNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDAN
                     LRAVVKRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEV
                     PKEALSLKDMDSINLANNYLIDDDVVNSLSHNGVDVNCDLNFIDTKDNQYKLDSKYHN
                     VDIAKGESIDLRKIITKKIDSYYKYWEVTDNLPKDLNFIVSVSDKSVLSCEDMIIKGN
                     KTGQAVVKVILSDKNDLNTSQEVLITVNVK"
     gene            complement(45774..46418)
                     /gene="trmB"
                     /locus_tag="MJE33_000239"
     CDS             complement(45774..46418)
                     /gene="trmB"
                     /locus_tag="MJE33_000239"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBY9646221.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            complement(46556..46876)
                     /locus_tag="MJE33_000240"
     CDS             complement(46556..46876)
                     /locus_tag="MJE33_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HBY9646222.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
     gene            complement(47329..47577)
                     /locus_tag="MJE33_000241"
     CDS             complement(47329..47577)
                     /locus_tag="MJE33_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBY9646223.1"
                     /translation="MQKTKAWGIIEPEKPESQQQMAHMDFAVNDLEEAVQYAIHCGAT
                     IAEEQFTDDWRVMIDPAGHPFCLCQMKSIMESSHFSLL"
     gene            complement(47608..48069)
                     /locus_tag="MJE33_000242"
     CDS             complement(47608..48069)
                     /locus_tag="MJE33_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBY9646224.1"
                     /translation="MKFGGVLLIVKDINKSKKFYEDVMEQKITLDLGEHVSFENGFGL
                     QSNYKAIIGYDLPIYQQSNNFQLYFEVNDIKQWESKINRIGNIEFLHYIKEYPWGQRT
                     FRFYDFDKNIIEISESMESVIKRFLKQGLALEEISKRTMYPVEFITQFQQL"
     gene            complement(48107..48940)
                     /locus_tag="MJE33_000243"
     CDS             complement(48107..48940)
                     /locus_tag="MJE33_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY9646225.1"
                     /translation="MLKKESIHFINKNRSLIVCEDNFVYLLPCIQLRNWISNFTISFP
                     NQTIISDNYTIIPHGSVTLVFFYDATGLHSLLFGPTTKPKTVGNIANRCDVILIIEFQ
                     PAGFFPLIGIQQSELIDKVVPFSIINTSLDLEIKKIFNESLSIDKLILKLEELLISNI
                     KIEYSYEFILAIQLIIQNSGNISSQEISKKVFYSSRHLNRLFNQYLGLSMKSFSRLVR
                     INKSIKLLNNNKTSLMSICNELGFYDIPHFIKDFKIVCGITPQKYRANMSDFYSEIAK
                     F"
     gene            complement(49214..49900)
                     /locus_tag="MJE33_000244"
     CDS             complement(49214..49900)
                     /locus_tag="MJE33_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9646226.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKDVSLQVMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNGADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILDIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALIGKPEILFADEPTGNLDSKTGIEIIDLLNKINRDNGQTIIMVTHSPEAAKSSSR
                     TIIVSDGLIV"
     gene            complement(49915..52392)
                     /locus_tag="MJE33_000245"
     CDS             complement(49915..52392)
                     /locus_tag="MJE33_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBY9646227.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFVSFSIGLSYE
                     SAQRKMAKGMYGTATISVQSKNPDILTNLEDIPDLNTIKSKVGVLESSAIYNKGGYYE
                     EFSLISADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRNTRGVNALLPKETLSKIINKGSGYTRVLIESEEEITENL
                     VNKLSEELSTEKYMVSNTINETKIISDARQKTMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTRILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPVVISPIGVIISVIVAIIVSSFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSILLPRISPENTLYLAGGFSLLGLIVATIVLIPLITDIMSIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGKMNEEFIEDVRHMDGIKKILPLYVMNNEISGNGVTLSRLEGTDNIKLYNSM
                     FGINYTNFEIKKQVIEAFNDKRSVILNEDTLKKVGLSIGDTITLSNDKYDFSYKIVGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDAVMIQIRYLFGDTYNWSRTVE
                     EFNNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKRRSIAMYKSIGLSDK
                     QNIKVTLIEGFTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            complement(52611..54014)
                     /locus_tag="MJE33_000246"
     CDS             complement(52611..54014)
                     /locus_tag="MJE33_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY9646228.1"
                     /translation="MHKPSYKTVFHSYIIFFMIFICLILTVIGIFLSLITIQKPNGEF
                     IRSDWPKKFTENFNEQIVFIDNKPQVKQSGLEKLRENQLWLQILDSSGNRVYGFLEPK
                     EHKDYYSNSELLALANQNNSSNGTVYFNTIKNNKNDYIYIIYFPLNISKVNMFFDGGK
                     FIGGKSIVFSIIGIIFLIILVSGIAYGYWVTKCISNITSAVSDISKRSYLPVNIKGVF
                     ADIYNSLNILNEEIHKSDEIQNKTDIMREEWISNITHDLKTPLSPIKGYAELLTDSST
                     NYSPEKVYKYASIMLKNIEYTNTLINDLKLTYQLKNGIIPLNKNNYDLVRFLKELSID
                     ILNYPEYESKIVEFKSNIDNINFDFDCTLLKRAFSNLIVNAFTYGYKNTKVCVHITLN
                     ERIEIIISDNGKGMSREEASNLFNRYYRGSNTKQKIEGTGLGLAITKQIIEVHGGNIS
                     VESIPGLGSSFYITFPI"
     gene            54512..55372
                     /locus_tag="MJE33_000247"
     CDS             54512..55372
                     /locus_tag="MJE33_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBY9646229.1"
                     /translation="MKKQKSPILLEMLGLFFGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTILDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
     gene            complement(55611..56168)
                     /locus_tag="MJE33_000248"
     CDS             complement(55611..56168)
                     /locus_tag="MJE33_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY9646230.1"
                     /translation="MSDSEYPQMKTLETRSCILRPASLDDVEDFFDCYKNKIVVKHLP
                     FSEHKSLEDTRKFIKSFFLNPYKKGKIGHFAIVYKRDNKVIGNMGFNNINPKALEAEI
                     GICINPSYWGHDFATEITKRVIQYGFRELNLNKIIAITYEENQNSKKSLDLLGFKCTG
                     KYIKKIHTGNTVKNVPCYQYELKKQ"
     gene            complement(56358..57245)
                     /locus_tag="MJE33_000249"
     CDS             complement(56358..57245)
                     /locus_tag="MJE33_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBY9646231.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENEKLFKLDIKEPIPKRNIGYCHLK
                     DISLSLATKEFLSMISNNI"
     gene            57364..58563
                     /locus_tag="MJE33_000250"
     CDS             57364..58563
                     /locus_tag="MJE33_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HBY9646232.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
     gene            complement(58757..61312)
                     /locus_tag="MJE33_000251"
     CDS             complement(58757..61312)
                     /locus_tag="MJE33_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBY9646233.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKENKKSKEYTVDRLGITKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETALEGFVNDYFQPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     TYINWPLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIEVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILIAVRVVHQLNKYSSIESMKNFKITNKKK
                     RILESVKHSNMTKVLSHKYMTQKKGMFIGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNVEGISKVYPVRYLFGAMQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGEIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKTMKPTDEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFDIENYNSVSIKKTEDSNS
                     EELSENIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFLLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINTPLYIGMIILNITISIVAVIIPVRQILKSDIISEINKN"
     gene            complement(61309..61995)
                     /locus_tag="MJE33_000252"
     CDS             complement(61309..61995)
                     /locus_tag="MJE33_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9646234.1"
                     /translation="MSILVTNNLVKHYGKGETKVKALNGVSIEIERDTFTAIVGTSGS
                     GKSTLLNIIGGLDNPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     VVVNTGGEVL"
     gene            complement(62072..62980)
                     /locus_tag="MJE33_000253"
     CDS             complement(62072..62980)
                     /locus_tag="MJE33_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY9646235.1"
                     /translation="MFILAFIICTVVIFIVSTKINRKRYDELNLMLNQILEGKEVTYP
                     DTKDTRASKISHQVKKIKDMIEIEVEQSKSEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKAEAKNIRINLEPFSDIKLVHNKKWTIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVENQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            complement(62982..63653)
                     /locus_tag="MJE33_000254"
     CDS             complement(62982..63653)
                     /locus_tag="MJE33_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY9646236.1"
                     /translation="MNLLIIEDDINLNEGLFYAFENDGFNVFKAYTKQEGLNIFNSKN
                     IDFIILDCNLPDGYGFDVCQIIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPF
                     SLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKECLELSSVEYKLLSYLIE
                     NKGQILLKEQILHHIWDSEENYVDDNIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYL
                     WNEVE"
     gene            63915..64979
                     /locus_tag="MJE33_000255"
     CDS             63915..64979
                     /locus_tag="MJE33_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HBY9646237.1"
                     /translation="MKRVDERNTMFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRP
                     NLCSEGTMTYNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQ
                     YGASVVGITKLKDYHIYTNRGRHEENYGEEINLTHKYAIVFGCEMDKEMINRAPMICE
                     VIETSKCYVDASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAI
                     LTNKDYGSRLRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKIGD
                     DGKYNWVIEHETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKGNNELIQKALD
                     EYTSKFGKRIFIPGNPSWLK"
     gene            65137..65823
                     /locus_tag="MJE33_000256"
     CDS             65137..65823
                     /locus_tag="MJE33_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY9646238.1"
                     /translation="MEKILVVEDDSILNKTLSYNLIEDGYIITSKFTAKSALKSIFEC
                     EFDLIILDINLPDKSGFELCNEIKGNYNIPIIFLTANDMECDMIKGYELGALDYITKP
                     FNINIFKQKVKAFLNHLTIKTKQDYYRDGYLEINFSELSANINGNQIIFTPLEYRTLK
                     LLTENPKSILTRKVLLEKLWDIDANFVDEHTLTSVISRIRSKIEKDNLKYIKTVYGMG
                     YMWLGERNEF"
     gene            65813..66844
                     /locus_tag="MJE33_000257"
     CDS             65813..66844
                     /locus_tag="MJE33_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY9646239.1"
                     /translation="MNFKKFSFSSIYLIIAVGIIFTAILFSISIYLITADLKLIALLL
                     IYTIVLCGFIALIIFLLHKKIVIFCSKICHTLDSMMNSEDNIEIELEEETILSRINHR
                     LIRMYEVIQENRNSIAIEKADLQELVSDISHQIKTPIANLKMINTTLLNQTFKTEIQK
                     DFLMDMENQLDKLDFLMQSMVKTSRLETGIITLSKKKNSIYETIATALSGILFDAEKK
                     NIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTNSNGKIRVATECWVMYTKIDII
                     DNGKGISESHQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSDIGKGS
                     TFSIFLPNK"
     gene            66926..67591
                     /locus_tag="MJE33_000258"
     CDS             66926..67591
                     /locus_tag="MJE33_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9646240.1"
                     /translation="MSILQVINLKKYYGQSSNITKALDGVTLSIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMTDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGETIDKDFINEIINMLSLEEKLYDMPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGNLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            67604..70021
                     /locus_tag="MJE33_000259"
     CDS             67604..70021
                     /locus_tag="MJE33_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9646241.1"
                     /translation="MFRNNNRTFIRKIALNDLKINKLKTYLSGIIIMISTCLLLTVTL
                     VSYNASVDLVNASPYHAIYKSVDEKAKNILYKNKNFSGVGTYKLIGSNKKTDCIMSIV
                     YADDTTIKLMNFQPLKGKLPTNKNEIAISEKYLQEFGLDKDIGDSIKLNYYDDITNKE
                     VQCDFIIVGFLENYYQDNAKQYYTVVSNDYFKNIASTPLQNKSNTFDESRPDTVDVLV
                     KLSEENTEKDSASIKTQLKKIGLSLGIKKYNIYLNDNYIESNLIDGEQIITVLVVGIV
                     VLFSSVFVIYSIFYISVVNLVQMYAKLKSLGMTSFQLKKIIFLQGNILSIIFIPLGVI
                     ASCIIAYIIQPLAWQMIADLFIILILSLVMFLTVRISLFKPARIISKISAIEAMQYTE
                     TKFRKKVKNFSYINIKNLALKNIESNRKKNLIALISLSISGVLFISIANLANSIDFKK
                     QLSQQFVYNEDYIISIKSDNLYEHITEIQQNNPLTDSLKNEIKSIHGVKKLIESKLIK
                     SNIIEPYIKDRDEKQFITLIKGITPELSKSLEEYIVSGKIDYTHLDSDSLIINKYRTK
                     YYGLNLKVGDTVTFNIFSGNDIIKKKMKIIGIIDNSNTEGMFFASDKTLESLTPYNTN
                     LDFSILIDKKYSKEVEEKLLSIIQKNSNLMLYSYEDDYRMITRAFQYIIIASYIFVGL
                     ISCFGILNMTNTLINSVLIRKKEFALLQAVGMTRKQLRNMLYREGLNISIKAICTSSI
                     LGYFGSNLLCTFIKDVIRLDFINFKFSIFTILIFSFVLIGIQVLVTELLVRNIEKKSL
                     TERLRSQ"
     gene            complement(70202..70993)
                     /locus_tag="MJE33_000260"
     CDS             complement(70202..70993)
                     /locus_tag="MJE33_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBY9646242.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYIDKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIEVFHKNTQSNENISITQGNAMDLSV
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFAAYVISDGCLIDEGFH
                     RGNINVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSAFNVTRLHYVASDGL
                     ALYMREAVDSMDDDAFALYLKYHLATCEREDLVGVTSHAIDIFRK"
     gene            complement(71026..71613)
                     /locus_tag="MJE33_000261"
     CDS             complement(71026..71613)
                     /locus_tag="MJE33_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY9646243.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNQYVVNYRNSKSVSPYLLLPLSYIEFAQEETYLFKL
                     LFINDLDLMMKEANDFYKEIDNEKKARLFSESIGIELECAKVIFLDLFLYTHGIAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKPDWNLSI"
     gene            complement(71789..73180)
                     /locus_tag="MJE33_000262"
     CDS             complement(71789..73180)
                     /locus_tag="MJE33_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HBY9646244.1"
                     /translation="MERLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFN
                     PKGRFDSYPISITVGILVACFVMFSERFDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSREIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSVMLFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIFSPYIPMCGPLAATLTGS
                     VAQRYKIGKEAMQSVFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCNTGVEKGLIGLMGGVIAAKGGTWGLAVGFILYFILIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            complement(73370..74668)
                     /locus_tag="MJE33_000263"
     CDS             complement(73370..74668)
                     /locus_tag="MJE33_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/BAAT N-terminal
                     domain-containing protein"
                     /protein_id="HBY9646245.1"
                     /translation="MKVNVYNVESFVDERLKIMVSDLIPNTKLKISVKMEFPWCKGEE
                     FSSYGVFCSNEKGEVDLDLVEPIEGTYKADNCMGLIYSLEKSKTEGKNFAENISIDKP
                     IIMNMIFETSIERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTILMLGGSD
                     GNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEVPLEYFEKVFEWISENEITST
                     KEIFVHGTSKGGELAFLLASRYEQIKKVVVSQPHIYCFQALNGLMSGNDTSSWSYKGE
                     PFPYIKVDNDIFFEEQKKNISKDIPFGFNSTYKKSLKRAENRKEARIKIENSKADILM
                     IAGKEDNIWNSYEACLEALEIFEENNYPYNVELLTYNNMGHPLPIPYIMPLKETLCMS
                     MSGGIFSSGGTVEGNSKGQFESWNRTIEFYKKPLSSDLNN"
     gene            complement(75027..75902)
                     /locus_tag="MJE33_000264"
     CDS             complement(75027..75902)
                     /locus_tag="MJE33_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HBY9646246.1"
                     /translation="MITFDVNGAIIRFDDTKNNYNKIRKIFKLYALEISKNFERDCLN
                     SFQNLKQISDRGLSLGEKYIEESFKKGIETIVSFGIITIDIDTFKSVYCEKYLDFKRL
                     FNNLNKTFIIPNKNKKNGHASFLEIESTIKKMSNYLYNDCFKIHYAVVDALIENRITA
                     VHSYIDEESIKKSNALFNNYKDGFIGKADECKVVKQIITLNPYREDVYEFLVKEDGDF
                     SKEIERLTEFLGYDIREYKASLMDIYINELMESDENDVENAKEKIEKYARYIGCSDEN
                     LYVARVNAIYTFANA"
     gene            complement(76095..76781)
                     /locus_tag="MJE33_000265"
     CDS             complement(76095..76781)
                     /locus_tag="MJE33_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBY9646247.1"
                     /translation="MFGVIANSLAIAGGCIVGLIIKGGLPQRVSDTIMNGIALCVLYI
                     GISGALEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(76830..77363)
                     /locus_tag="MJE33_000266"
     CDS             complement(76830..77363)
                     /locus_tag="MJE33_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBY9646248.1"
                     /translation="MSDLNNKKEQDENESSMNETKIYDILDKLNVEYDVVEHEAVYTA
                     EQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSRLSFASP
                     ERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVIKEDYLNFHPNINTKTVNISRKG
                     FEEFLEYLKYPIQSIDV"
     gene            complement(77407..78039)
                     /locus_tag="MJE33_000267"
     CDS             complement(77407..78039)
                     /locus_tag="MJE33_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HBY9646249.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNENIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYDTSVFE
                     VHHILFEKGKIIIENLTNLENVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(78129..79208)
                     /locus_tag="MJE33_000268"
     CDS             complement(78129..79208)
                     /locus_tag="MJE33_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HBY9646250.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLENDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSNVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLRFIKNI"
     gene            complement(79341..80687)
                     /gene="pepV"
                     /locus_tag="MJE33_000269"
     CDS             complement(79341..80687)
                     /gene="pepV"
                     /locus_tag="MJE33_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBY9646251.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGQGEKMIGILGHVDVVPEGDLESWNYPPF
                     EAVLEDGKLYGRGTQDDKGPTISAIYAVKALMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELDKLNFEYTVEGNKICIIGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDNEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTLLVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKATQIYALSIYELLKI"
     gene            complement(80756..82036)
                     /locus_tag="MJE33_000270"
     CDS             complement(80756..82036)
                     /locus_tag="MJE33_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HBY9646252.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEEIKDKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDVDFLEDGNL
                     DLEGIKSKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYDDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(82163..82423)
                     /gene="hfq"
                     /locus_tag="MJE33_000271"
     CDS             complement(82163..82423)
                     /gene="hfq"
                     /locus_tag="MJE33_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HBY9646253.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNNR"
     gene            complement(82537..83472)
                     /gene="miaA"
                     /locus_tag="MJE33_000272"
     CDS             complement(82537..83472)
                     /gene="miaA"
                     /locus_tag="MJE33_000272"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBY9646254.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDMNAEIISADSMQIYEYM
                     DIGSAKVTEEEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIHEINEKEKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQKQLAEKGIDYMHNKLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSSDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMIKNG
                     LVEEAKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTAKWFDLDQYENIDELKNEIILYIKDSIK"
     gene            complement(83510..85477)
                     /gene="mutL"
                     /locus_tag="MJE33_000273"
     CDS             complement(83510..85477)
                     /gene="mutL"
                     /locus_tag="MJE33_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBY9646255.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGIGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMTTKTKDEIIGTKIYVEGGKIISKEPIGSTNGTTIIIKDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNNDLLESTPKNSNITKGKDEVIEVVTLSSE
                     KPINEFQSVSEVLNASVEDDVKNINYLSEDSANDNIQEEFQVDGIKNEGNYYLGDSIK
                     DSEEEYLCSSKRKFSLYGYFVIGVVFNTYIILSKDDSMYLLDQHAAHERILYERYMEK
                     FYRQDINMQILLDPVVIEVSNIDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIF
                     GVPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLE
                     KCENPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            complement(85488..88331)
                     /gene="mutS"
                     /locus_tag="MJE33_000274"
     CDS             complement(85488..88331)
                     /gene="mutS"
                     /locus_tag="MJE33_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBY9646256.1"
                     /translation="MQIDMNKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIEKLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYIYNTQKQITSNINNINIYNSSEYMVLDMFTRTNLE
                     LTQTIRGNKKKGSLLHVLDKTSTAMGGRLLRKYVEEPLINKSKIENRLDVIEEIKDDL
                     ILREDLNDILKNIYDIERICGKIAFERVTPKELIHLKNSIEKLPNLKDTINLSNAKIL
                     KEYVSEMDKLDDIYNLIDEAILEEPTITIKDGNIIKSDFSDELKELREISKNGAFLVK
                     EIENREREKTGVKSLKIGFNKVFGYYIEITKANFKQAKLDETYIRKQTLSNAERYITP
                     ELKEIEEKILHAEEKIKSLEYEIFVEIRDTIYKNIDRIQKVAKTIANIDVFVSLATVA
                     HINNYVKPAINENNKLDIRNGRHPVVENIVGEENFVPNDTYLNRGENIINIITGPNMS
                     GKSTYMRQTAIIALMAHIGSFVPAESADIPILDRIFTRVGASDDLSQGQSTFMVEMNE
                     VSLILKNATERSLVILDEIGRGTSTYDGISLAWSIVEYIQKNIRCKTLFATHYHELTD
                     LEEEFKEVKNYSIAVKEDGEGIIFLRKIIPQGADKSYGIYVAKLAKLPDEVIERAKYI
                     LKDLEKNHVYNSVAINGDKENNNIINSNLDEELVKVNQSNFKNKYESLKIEHELIVKD
                     YKHIKKDYDKLNSKFKALNDEVALMKQNDDKEKINQEDSVKEVALTQISFDSVNRDIL
                     SEEILNLDILNMTPLDAINSLYNLQRKAKEIKS"
     gene            complement(88492..89226)
                     /locus_tag="MJE33_000275"
     CDS             complement(88492..89226)
                     /locus_tag="MJE33_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9646257.1"
                     /translation="MLTQMNFYHNKKVKVSIENVSKTYKDIEVLKDISIDVYEGEFVS
                     ILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLIL
                     KKEKKSKARSMVKPYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDEP
                     FGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIVKKEF
                     VIDSRKLNEDNLENIIKLKKEIISLL"
     gene            complement(89195..90208)
                     /locus_tag="MJE33_000276"
     CDS             complement(89195..90208)
                     /locus_tag="MJE33_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBY9646258.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKDTSSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVATVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSPSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKNNPELAKKFLKATTKGYEYAIKNPEESAKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPQ"
     gene            complement(90230..91000)
                     /locus_tag="MJE33_000277"
     CDS             complement(90230..91000)
                     /locus_tag="MJE33_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9646259.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSMVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSIHAVVTIGEAILGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYLNLFKT
                     MDSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQAVKKVVIRHRLID"
     gene            complement(90975..91265)
                     /locus_tag="MJE33_000278"
     CDS             complement(90975..91265)
                     /locus_tag="MJE33_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HBY9646260.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     regulatory      complement(91396..91495)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     regulatory      92115..92204
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            92257..92433
                     /locus_tag="MJE33_000279"
     CDS             92257..92433
                     /locus_tag="MJE33_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646261.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
     gene            complement(92548..92904)
                     /locus_tag="MJE33_000280"
     CDS             complement(92548..92904)
                     /locus_tag="MJE33_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY9646262.1"
                     /translation="MDIDNNFEEFPNIEERQKDFNCSIGFTMTVIGSKWRAIILWHIL
                     KQEPIRYSQLKKEVVHISHKILSQELKYLERDGLIKRISYPTIPPKVEYLSTKRGKSL
                     ESILLELCNWGKQYMR"
     gene            93160..94073
                     /locus_tag="MJE33_000281"
                     /pseudo
     CDS             93160..94073
                     /locus_tag="MJE33_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061260.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
     gene            94304..94915
                     /locus_tag="MJE33_000282"
     CDS             94304..94915
                     /locus_tag="MJE33_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HBY9646263.1"
                     /translation="MINDIKNKFKNYKPYINGYKNMKRASVLIPIVEINNTHYILFEV
                     RSKNLKHQPSEISFPGGKIESGETPYEAVIRETCEELGTFSDNIEVISSLDLLITPVK
                     FIIHPYLGYIKNINNLNINKDEVDHTFLVPIKYLLENPPNTYSNTVKVFPDENLPYGS
                     IPNQKDYKFESGSYPILFYKYKDYVIWGMTAKILENFLNFLSE"
     gene            95213..95551
                     /locus_tag="MJE33_000283"
     CDS             95213..95551
                     /locus_tag="MJE33_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HBY9646264.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSKKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVPFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
     gene            complement(95958..97394)
                     /gene="miaB"
                     /locus_tag="MJE33_000284"
     CDS             complement(95958..97394)
                     /gene="miaB"
                     /locus_tag="MJE33_000284"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HBY9646265.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPDMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERVRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPG
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHDRFNRVLEAVNRISAEINDGYKDRIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGG
                     NDDLIGKLVMVKITEPRTFSLNGILVNN"
     gene            complement(97642..99114)
                     /locus_tag="MJE33_000285"
     CDS             complement(97642..99114)
                     /locus_tag="MJE33_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HBY9646266.1"
                     /translation="MKFYKRILTLTLSISFVFANIQLVDAISSVEKIQGKNKYEIAGK
                     IADKNAYKTAYKTAYKTAYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPT
                     ETSARLKNVSKVYIIGGTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSI
                     KKVNTVMLTNAYKGEADAISIASVAARDKAPIILTNGQSIPFSTSGLKSYVIGGTASM
                     STTLVNNTKSTRLGGSTRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHGP
                     MVLVNDGSNKSILKNAKKITSIGYIDSNIVQQCLNITNGIGDINTGVVKNVKPTTKTI
                     KDGMYKVGKDISAGEYLITSNSGSYASYYEVTSDSTGNADSILSNDIFSGTRYITLKN
                     GQYIKIEDSTMTLAKYAKAQKAKNGKFGNGMYKIGLEIPAGEYIIMSNSSDAYYEVRN
                     DSLGNAEGIVTNDTFSGRRYITVEEDQYLILNDCYLIENE"
BASE COUNT        35781 a        14323 c        13145 g        36282 t
ORIGIN      
        1 aataaaactt aaagaaatta aacaatttct aaaaatacct tgaaaataga accttatttg
       61 ccatttataa attatataaa aagggattat ataaagtcat atatgaaaaa actatattat
      121 actatctttt ctttttggaa taaactatac tttaaatata gatatttaat ttaaataaat
      181 tttacagtat taatagtaaa ttatcttaga aaggaatgtg ctttatatgt ctacagacaa
      241 agttgttgga atcatagatg aagaaacggc agaacttgca ggaattgaat atactggtaa
      301 aatttatgct tcttctggtg ttataaaaca tataaagaaa aaacatagat gtcaactgag
      361 taaggatatt tttaatgata taatagatac tataaaaatg gtattaaagt cacctgaata
      421 tataggaagt catcctaaaa aacctggaaa aagtgtggag tttataaaaa aaattgatga
      481 ttatatttta gtagctacag aattagatac aaaaaaagga tatttatatg tatcttcaat
      541 gtatgctata aaagaagcaa aattagaaag tagagtaaac agtggaagag taaaacaata
      601 tgatggttaa cataatatta ttaattttcc ctaaaatatt gaaaaatatt attatataag
      661 ttataatatt aatatacacg tagatattta ttagttagat ttgaggttgg aaaaggcacc
      721 caatacgcta ctatagagta gttaatagag atgcaggata cgccgccctg ccaaatctaa
      781 ttaaattaaa aagagaggat taattcctct cttattgttt ttttgagata ctaatttaat
      841 ctatatagaa agattgaagg tcatttatac taaaagtttt cagttatata cattaaacta
      901 attaaataag atatttataa tctatatgtg atacatatta tatattaata aaagaaaaat
      961 atatagaaaa tccttataaa aatttaaatt gtaatgaata ggaagtaatt aaaaagaatt
     1021 tttattgatt tttaaaaatt tagttagaaa ctatatcatt atggtaaaaa atgtattata
     1081 atgtttagaa atattataat aaggagcgat taaacatgaa tctaacgaag aaaatagaag
     1141 ataaaagaaa agctaaacag agagctgaaa aagttaagaa agctaaaatt gctacagctg
     1201 gtgtggtatt aggagctgtt actggtgcag tgagtggagt acttttggct cctaaatctg
     1261 gaaaagaaac aagagaggat ataaaagatg cttcacaaca aattgctgaa aagataaata
     1321 tgaaaactgt agatgtaaaa ggcaaagtat ctgagaagct agaggataaa aaaggaaact
     1381 ttatagaatc aaagaaaaaa ataaaaaaat atatagataa caaaaagagt gttgaaaatt
     1441 ctagtgagga agataaaata atagatatag ttgaaccagt tagtgttact gaggaataat
     1501 atggacaata caataacgat taacataagt ataatatatg tattattagc tatacttgct
     1561 tgtattgctt tagtttattt aattattgta cttgttaaac tacataagat tttaaataat
     1621 gttggaacta tgttaaatga aaatactcaa aatataaata caactttaaa taatcttcct
     1681 aatgttgtag aaacttttgg agaagtagga gaaaatatga aagatgtaac agaagtagtt
     1741 actgaagtta cagcagattt tcttgttact aaagacagtg tcaaatcaaa tatggatgtt
     1801 atagttgata tattgactat aattaaaaac atttttctag ataaaaaata ataagttaat
     1861 ataaaataag cctataagat ataatttaaa cgaccttaaa atagcattta taaggtcgtt
     1921 ttttattagc ttgatttgac actatgtgtt tttaataatt ggtaatattc ctctttatca
     1981 tttgaacata tatatctatg cttgttgttt caattacatc cataagaaca tctttacatt
     2041 caagctcaaa atcttccatt ccttctaaat attcatcatc ttcatcaaaa aagatataag
     2101 aattatatag ccattcttca aatttttctt caagtatatc ccaatacgaa ctccaaaata
     2161 atccacaacc tgatatataa gatgctctac tttgatttat ataaacctca tttagaagct
     2221 tttgtaatct actgctataa agaaaatctg ctcttccact gcttgatatt aattcattta
     2281 aatcttctat tgacattaat tcaactttac cacttttatc ataaggagta taattttcat
     2341 caacccattt atgtggtttg ttttctctgc atactgttgc tatcaactct acaacttttg
     2401 ttcttatcat ttttttatcc taacttattt attttttata aaataatcat gattttataa
     2461 tttatctatt tcttgtaatg ctaatttaac agtttcatat ctatcagcac catttattac
     2521 tacatcacct tttacaagtt tattagcttc tccacctaca acataaacct ttttacctaa
     2581 accttgctta taactagacg cctcaattat taaagaatct ttcaattgcc aatgtaaaat
     2641 ctgagcagcc actttatcag catcacctgt ataaacgatt acattttcat acactttatt
     2701 ttcccctccc ccttgagaat ttgatataga tttgtttagt ataccttctg ctatcaattt
     2761 agcaacttta tctttattat taacataata atctgtatct gctttactat ctacaaaaca
     2821 tacttctatt aatattgctg gtgcaatagt atgattaagc caataaagac ctctaacatc
     2881 agactttgca cccctatctt tgaatactgt agctaaccta gtattcactc tttctgcata
     2941 ggttttgcca ttattagtct tatatattgt ttctgtccct actggagttg aagttgtagc
     3001 atttgaatta aaatgaatct gaacagccaa atcagtttcc tgtttatttg ctatagcaca
     3061 ttggtctgct aaatgattac ttgattcatc aactcttcct gtataaacag tagctccacc
     3121 tattttaagc catttaacaa ttaagtcatt cagtattctt gtttctttgc tttcgtttat
     3181 atacccaaca gctcctgtac cttttccaat taaagtgtgc cctggtatta ttactacttt
     3241 caaactaaca tctcctttac aattaaattt aacttctatt taatatatgc ataacctaga
     3301 attttgtttc tttttatatc ttatactttt attataacct attttgtaaa aatatagaat
     3361 attaagtaaa taatgttaaa attataattt tgtatataag acattacttg attgcaataa
     3421 ttttatagca tattcatcta ttctatatcc agttctataa tatatatgtt taattccagc
     3481 ttgaattata gccttagtac aatttaagca aggaaagtga gttacataaa tagagcaatt
     3541 tttaactgaa ataccttctt ttgcacaata ataaagggca ttttgttcag cgtgaatggt
     3601 tctaatacaa tgtccatctc tcatttcatg accaacttca ctacaatgct tatctccaga
     3661 aatactacca ttatatccag tagacactat tctgttttct gaattgacaa ttatagctcc
     3721 tacataagct ctatcacaag ttcctctttc agctactgtt tcgcacagtc tcataaaata
     3781 ttcttgccat gagcatctat tttcaagttt tttattcaaa ttatatacct ccaaaactta
     3841 tatctaatat ataagattta taccttaaaa ataatattaa aaacaaattt atacgataaa
     3901 taaaatttat taatactgtt tttagtctaa attatttcat tttaaattaa agttacaagg
     3961 aatctaaaat tatatatatc tttcttcgta atattaatgt tacaatataa aaaactgtct
     4021 caataaaata gagacagttt tacagtcaaa gtttataata caagttcata tttttcttta
     4081 aattctttta cagcttcggg ccatgccttt tgttctgtaa tacctttttc tttagctatt
     4141 ttaattgcaa tctctcttat cattgcagcg ccttctaata ttcccatttt taatctccct
     4201 tcaaatatct ttttgtatag aatataaata gtgctaagat ttattacatg ctgcatatac
     4261 aatatctata caacacataa cttgtaaatt ccttttattt tcatacgtct ttaaccgaca
     4321 tataattata ttcaataagt tttaaaatta tgaactcaat tataattatt tttatttttt
     4381 tagaatctaa acactaaaaa ttgtaggtaa atttgtctta tgcctttata aatatttcta
     4441 cagttttaat tactggacat atctattttt attataaaaa gcgcttaaaa atcgagaata
     4501 tccgcataaa aactgtatat aagcaaaacg gttaaaggaa aaagaagaaa tttacttgaa
     4561 tgttaaaatg cgttgcttga agcaatgggc agttttgcct ataatatcgg taacaactga
     4621 aagaaaggtg gttagcccta atgttaagcg aaaacattaa aacaatcagg aagtcaaaag
     4681 gactttcaca acaagaactt gctataaaac tgaatgtagt gcgacaaaca atatctaaat
     4741 gggaacaagg actgtcagtt cccgattctg atatgttgat ttccatatca gaggtgcttg
     4801 aaacgcccgt aagcacttta cttggagaaa ctattgttga gtcggaagtt gatagcttaa
     4861 aagtgatttc tgaaaaatta gagattataa acttgcaact tgcacaaaga aaaaacatga
     4921 gaagaaaaat gtttcattgg ttatttattt tattatgtgc agtcatatta ataggttttg
     4981 tggtcttagg gggactaaat agtccttact tggattggaa ttataccgac cctgaaaccg
     5041 ctgtttttgg agtagctttt catgcttttg agtggctatt tgtcagatta gtaccgattg
     5101 tccttatagg agcaattatt ggaatctttg tgacacgtaa gaaaatatga agtaactctg
     5161 ctttttataa tacagcaagc gttccttaaa agcgtcattc tacatggcaa tcgaggacga
     5221 ctatgtaagg aagaaccttt ttgatttcca actaagcgaa gttattgagg acgacagtaa
     5281 gaccaaaacc gcattaagta aagtacagga agcaagactg ctggacttat actctcatgc
     5341 gtcagatgca ggaaatgcga ggtctggtag catgatttac aacgatttgt acaacgactg
     5401 tgagtagaaa tatatggaga taagtattat ctacgaaatc agaaaagaaa tgacttgata
     5461 atttactcat gttataatta ttgatgtgaa attaataaat cgaaattcct tttttggatt
     5521 taacaatact gagtaaggaa aaagtgaatg acttttgcaa ggaggtgtta atatcaggca
     5581 agtaaagtct tttgtttttc cagttatatc cttaatattc ttagaccaaa ttagcaaagt
     5641 tcttatagga ttattcttaa tggactttga aattgatata attgggaaat ttttaagatt
     5701 caatcctgtt caaaatacaa atctctctta tgggggaaac tttattggta ttctatctaa
     5761 tttatgggta ttggtattgt ttaacatttt agttatatta gttattatat ctggatatgc
     5821 tttttataaa tcaaaaaatg aacaaacaag ctattcagta aaagtaatta tgtcttgtgg
     5881 acttgctggt acaatatgta gcttgataga taaattattt tggggaggaa gtttagattt
     5941 tttgcagata ccaagctttt ttattttcga cttaaaagac tgctacctta ctgttgcaga
     6001 aataatattt gttgtcatag gaattttgca taatagagaa atatcaatga aagaatacat
     6061 atatttttgc tatcgtcagt ttaaaaaata aatttagaag ataaatttaa gtttcaaggt
     6121 aaacttattt aatcacagca aatagttggc ttacttaagc ttttgggcta aactgtgaga
     6181 actaattaag tctctgctat ctaacaaaca gagcacaaaa ataaaaatta aatattaaat
     6241 ataaagcaca gttaattttg attaaaaagt tgactgtctt ttcttttgta gaaatttaag
     6301 cataggtaat aaagaaatga ctgcatttaa gcattatgta tataattgga ggcaataaaa
     6361 agccagcaaa gcccataaaa tagagctgtg ctagcaacat gatgagttat aagaagataa
     6421 tcaaaaactt acgctttatt ttaatttaaa aaaatgatta ctgtaaagaa atcatttttt
     6481 attataatca ctgatacatc aaatattcta aataaaattt ttatttatct ggaattgtat
     6541 attttttcta taatattaac tacagcgtac attgcccaag cacagatagc caatacaaat
     6601 actcccatca ttactaaatc taatttaaat acttgagaac catatactat caagtagcct
     6661 attccatatc ttgaaactaa aaattctcca actattacac ctacccatgc cataccaata
     6721 ttaattttag ttaaatttat taaatttcct gtgtttgcag gtaatatgag tttaaataat
     6781 atttgagatt tagtagctcc aaagcttttt aacatcttaa ttttttcttc atctatattc
     6841 ataaaataat tataagctga aagtatagta acaacaacag aaatcgttac agctgttacg
     6901 acgattcctt ctattccagc acctacccaa actataatta taggagctaa agcagtctta
     6961 ggaagagcat ttaaaactac caaaaatggg tctaatattt ttgataattt ttctgaccac
     7021 caaagagcaa ttgctaccaa aatacctaat acagtaccta taacaagacc aagaacagtt
     7081 tcatatacag atattccaat atgtttaaat aattgaccac taaatgcata ttgaataaat
     7141 aaattgtaaa tatcacttgg tttgctaaat agaaatgtat ttataacatt taagtcagct
     7201 aaaagttccc agattacaat aaaaccaatt aaaattaata gctggtagaa taatacattt
     7261 cttttttctc tttttacacc tttaacatag tttatatatc cttcagatct tttattaagc
     7321 ctcatgtgta tctagctcct tccataatat gtcgaaatac ttttgaaaat caggaacttt
     7381 acgtgttttg agaggcgttt ttgttccatc catgttaagt tcaatatcgt aaatattttt
     7441 tatggtagct ggtcttttag atagtattgc aaccttatct gatatgctta ttgcttctgc
     7501 tatagctaaa aaagtgcaac taattgctta aagaaacaga attatccact tcagcaccca
     7561 tataaacaat attaagattt cctaaactgc tatcccaaac gatactatta ataatagtag
     7621 ataataacaa tctcttatca tctaaactgg cattatcaac ttcatcatta aatctcttca
     7681 aattactaac aataatatca atattaatac tgtcactact aatattgatg ttactagatt
     7741 ccaaaatatt aatcttatcc tttaaaatac taatctcatt atttaggctc tcaatctggg
     7801 aaactatata atcagaagca gtactaccag tattttttgc aagctctata actaaatttt
     7861 taataagtct ttccttatct aaaatttgat ttttaagtaa attagtctca gatttaaaaa
     7921 tattaaaaac attatcagtc ttaatgttat tactattgaa tgcacttata atagactctt
     7981 ctttacaatt cttaatttta tctataacta aactttcaac taatggacca ttaagatttt
     8041 tacagttgca actatcagac ccaagtgttt ttttagtacc acaaatataa taatacttta
     8101 aatccttgtc agtttttttg cctttatagg taattctcat attagaacca catttaccac
     8161 attttataag accagataaa agagcagttt ttccagttga tatccttgga gttttatcac
     8221 gatttttatc taattgattt tgtacttcaa gccatatttc aggttctatg actccatggt
     8281 gttttgcaat tgctgctatt gagttagatg tatttttagc ataagtaagg attccacatt
     8341 ttctatctgg agtacctaca acatccatac cactattttt aaggtaatct aaaatttcat
     8401 cattagcttt tacatatgct ggatttctaa gtattctaga taaagaacta gggtcaaatt
     8461 taccaccacg aggacccctt attccattat tatagacata tttatataac ttagataagg
     8521 acctaagttc taaatattta ttgtatatta gctttacaat ctccatttgt tcatcattta
     8581 cttgaagctt atacatactt ctttctttta gattttcatc taagaatgtg attttttcac
     8641 tgttgaatcc atgaggtgta ttgcctccaa gccatctacc actacgggct aattcataca
     8701 tattgtcttt tatacgttct gctattgttt ctctttctaa ttgagcaaat acacttgata
     8761 taaacatcat agctcttccc attggagttg aagtatcaaa ttgttctttg atagatataa
     8821 atgatatgtt tagttcattt aatttttcta ttaaagtaga gaagtcagat acatttctcg
     8881 atatcctatc taatctataa caaactaggt aactaaattt cttatctttg gcatcctgca
     8941 tcattttctt aaattgtggt ctatctatat tgctaccact aaatccctca tcttcatata
     9001 tcaaaaaatc ttttacacca atagactttg cataatcaat acataattgt atttgatttt
     9061 ctatagaatc accttttcca gtaaatcttg attttcttga ataaattgca gctttcaaaa
     9121 ttaaaacccc ctataagtta ggatatatat ttgaaattat ttatatacaa ccttttaagt
     9181 aaacttatta gaatgaattt attaattagg taaatttata taattctact ttttataata
     9241 tatgactatt tattttattt ctttactatt caagttctaa atcaataata aattttgata
     9301 tttaattgta tcaggacaaa tctctaattt catagattat taatataaaa tcataggtta
     9361 gtgtgaaaaa gttgaaattt aaatatttta aaagataatt tttttgaact aataggtata
     9421 acaaaaattt atagggggat tgcatatgga aatcaatgtt atagaaattt tccctaaaga
     9481 taaagctaaa cttaataaaa tagaaatgga taaagctagt tggtttgtaa atataataag
     9541 taaaaaatat cctaaagaag ctttaaatga agcatttagt actttagaaa aagaattaaa
     9601 tataagtaaa gctaatacat aagctttgat tattgatttt atatactatt ggttttatac
     9661 gctatgactt gacattatat taatcaaata gtatattttt aaatatagca caatgaagtg
     9721 ttaaaagagc aatggagctc catagataaa gtctatggag cttttttgcg tatttaactt
     9781 aaggaggata ggactttgaa taactttgag attaaaacta gaataaaatt tggagaaggc
     9841 tcactaaaag ctttgcaaga attaaaaaac aaaaatgtac taataataac agaccctttt
     9901 atggtgaaat caaaaacaat agataaaata acatctaatt taataaactc aacatatcaa
     9961 gtttttgaag aaatagtacc agacccacca atagaagtag tagtagcagg aatagaagca
    10021 tttaaatcta taaatccaga cgtaataata gcattaggtg gcggttctgc aatagatgca
    10081 gcaaaatcta taatggactt ttctaaaaaa atattaaaaa ttaacgaagt agagtttata
    10141 gcaataccaa caacaagtgg aacaggttca gaagtaacat catttgctgt aataacagat
    10201 agtcaaaaaa aatgtaagta tccattagtg agtgatgatt tacttccaga tatggcaata
    10261 ctagacccag agcttgtaaa aactgtacca aattttataa cagcagatac agggatggat
    10321 gtattgacac atgctataga agcttacgta tctacaaatt ctaatgactt ttcagatgca
    10381 ctagctgaaa aagctataaa gttagtattt aaatacttaa taaaatcata taaagatgga
    10441 aatgacatag aagctagaga aaaaatgcac aatgcatctt gtttagcagg tatggcattt
    10501 aaccaagcat cacttggcat aaatcattct attgcacatg tattaggtgg aaaattccat
    10561 ataccacatg gaagaacaaa ctctatatta cttcctcatg taatagaata taattcatca
    10621 atagtaggat atactaacac aacttactct gatacagcta aaaaatatgc acaaatagca
    10681 gaattagtag gacttgggag tgctaatata agaatatctg ttagaaattt agtaaatgaa
    10741 ataaagaaac tacaaaaaga aatgaatatg ccaactaaat tgacacaatg caaattaagt
    10801 ttagaagata taactaatga agaaagagaa atagcaaaac ttgctataaa agatacttgt
    10861 acattaacta atcctaaggt accaactgaa gatgatataa taaacatatt aaagaacatt
    10921 aaataactac attttattac atgtaaatat aatattatac aaatagggaa aatgttttta
    10981 aaaatataaa ttatatattg acaaatataa aacataaaac tataatgaaa gtatcacaaa
    11041 ggcgtgatac tttagtagaa agcaatggag cttcttagga atattaatat tattcttatg
    11101 gagctccttt tattttaatc aaatagcttt ttaataaact atattcaaag aaagcaggga
    11161 ataaatttga ctgaagaatc taagcaaaga gttattcaag aatatgttcc tggaaaacag
    11221 gttacattag cacatatcat agctaaccct aatgaagaca tatataagaa gttaggatta
    11281 gtacttgata aaaaagatgc aataggaata cttacaataa caccaagtga agcgtcaata
    11341 atagcagcag atgttgctac aaaggcttca aatgtatctt taggttttat agatagattt
    11401 agtggttcag tagtaatatc aggtgatgta agttctgtag aatcagcgtt aaatgatgtt
    11461 ttagaagtct taggaaatat gttaaacttc tcatctacaa aaatcacaag gacataataa
    11521 tacaatgaag aatataatat ttatgggaaa gacaggtagt ggtaaaacta ccttatgtca
    11581 aaaactagac cagttagaat taaagtataa aaaaactcaa tcagtggaac tttataataa
    11641 tgcaatagac actcctggtg agtatatgga aaatagaaac ctatataatg ccttaataac
    11701 aacatcagca gatgctaaaa ttatagcaat agtgtatgat gccacacaag aagaaaatta
    11761 tatagcacca ggttttgcca gtatgttttg caaagacgta ataggaatta taactaaaat
    11821 aaataaagct actgaaaacg aagtcaatat agcttatgaa agactatcac tagcaggagc
    11881 aagtcaaatc tttaaaatag acacagttga taatattgga cttgaaaaat tatttaaata
    11941 tctagaaaaa tataaaaata tagatgaagt ttaaggggaa atattttttt attcaaaaat
    12001 attattaaag tatgataaaa taaaaaaatg tataaactat tttaagaaga tttaatcttt
    12061 aagcgaggta ggaacatggg gaaaaaaata ctattggttg atgatgaacc actgttaagg
    12121 atggatacaa aagatatatt agaaagtcat ggctatgaaa taataggcga agcaagtgat
    12181 ggatttgaag ctgttgaaat gtgtaaaaaa cacaaacctg atttagttat aatggatata
    12241 gagatgccaa tacttgatgg tataaaggca ggcaaaataa tatgtaaaga aaatctagct
    12301 ggtggagtac ttttattaac ttcatttgaa gataaagaat acgtagaaaa ggctaaaagt
    12361 ataggtgctt atggatattt agtaaaaaca gcaagtgaaa aatcacttat cccaaccata
    12421 gaaatgtgtt taaataaggt aaatgaattt gaaaaaatga aaaaagattt agaaaaagta
    12481 aattctaaat tgaatgaaag aaaattagta gaaagagcaa aaggtatact tataagagag
    12541 tttaagattt ctgaggatga agcatacact agaataagaa aattaagtat ggataaaaga
    12601 actacaatga tggaaattgc caaaatgatt ataataggat acgatgacta atacgataaa
    12661 aaaaatatca actgcacaaa catacttaaa taataacgaa attgaaaaaa ttatagaagt
    12721 atcaaaatcc ttagatttaa tggcttcttt ttatgaagct gatgttttta tagatgtact
    12781 aagtaaattt gatgatgaag caattgttgt ggcacatggt agacctaaag aaaagtcaat
    12841 gtactgtgaa aatgttatag gtaaaaaggc tttaattgaa aatgaaccag gagttataaa
    12901 gtgcttaaaa acaggagaaa gagtaagaga tattaaagct ttaacacagg aatataaact
    12961 ggtcaaacaa aatattcagc caataacact agatgacaaa gttttagcag tactaatagt
    13021 tgaaaaagat ataagtagtg aagttaagaa tgagtttaat gctagtgaag acgaagaaaa
    13081 taaaaattta catcagttta taaaacttat tggaaataat gactttttag ttgataattt
    13141 aaattcagcc atattaatat ttgataaaaa tggaaaatta aaaattaaaa atcgtattgc
    13201 aactgagata tacaaaaatc ttgggtttgg atatgatata caagatatgc attacaatga
    13261 tttagatata gaaaccaaca cctttgaaaa tatattaatg gatgaaaatt atgatgagac
    13321 aaatgaagtt tcaataggta atatgttttt taaaataaag accatataca tcaaagatga
    13381 tgaatttaag gtttgcaaaa tagtacaaga tataagtgat ttaaagagaa aagaagaaga
    13441 attagttttg aaaacagtgg ctataagaga agctcatcat agagtaaaaa ataatcttca
    13501 tacagtaata tctataataa aaaaacaaag tagattatct caaaatgatg aagtaaagca
    13561 gtgtttagat aacataataa atagggtatt tgcaatactt tcgactcatt atttactatc
    13621 taaagaagtt gataataata tatctatacg agaaggtatc aatttattag tgtcaaatat
    13681 tcaaggtggc tatttattta gtaaggatat agatatacgt ataactggtg aagattttaa
    13741 aattagtgga gaaaaagcaa cagcaatact tttggttata aatgaaatac tacaaaattg
    13801 ttatgaccat gcattttcgg atagagaaca tggtactata caaatattaa taaataaaga
    13861 taacgactat agaaatattg ctattataga tgatggaatt ggatttgata gtaataatat
    13921 aaataaaaat agcttaggga catatataat agatagttat ataacacaga tgttaaaagg
    13981 aaatatagag agaaaaagta gtgagaatgg tacagaagta ttaattacta tacctacaca
    14041 aattaatatt taaataataa ctcatctaca aagtagtagt tttaattgag caaagaggct
    14101 taaggttatt aatagattaa gttaattgaa tgtatttata aaaattataa gtatatattc
    14161 aatttaacag gtatttatta taactttaag cctctttgct tgtttaagac atacttaaat
    14221 tttaggaggt gagaaaatga gagaagagtt attaagtgtt ggtattgaca ttggaacaag
    14281 tactactcag ttaatttttt ctaaattaat agtagaaaat actgaatcaa atttttcggt
    14341 acctagaata agtatagtag acaaagaaat tatttataaa agcaaagttt atttcacccc
    14401 tcttatttca aataatgaaa taaatggtca acaaataaaa gatatagttg aaaatgaata
    14461 taaaaaagca aatatatcta aagaagatat acatacagga gcagtaataa taactggtga
    14521 aacagctaga aaagaaaatg caaatgatgt acttcatact ttgagtggat ttgctgggga
    14581 cttcgttgtt gcaacagcag gacctgattt agaaagtata atatcaggaa aaggagctgg
    14641 agctcatatt tattcaaaag aacattcaac tacaattgta aatatagaca taggtggggg
    14701 aactagtaac atagcctcat ttaaaagggg agaagtaacc gatactggat gtttggatat
    14761 aggtggaagg cttgtaaaga tagataaaat aagtaaaaaa ataacttata tatcacctaa
    14821 aatagataaa atcataaaag ataataattt aaatcttcaa gtaggcacaa tggctactgt
    14881 agaaaataca aagccaatag tggatataat ggttgactta ttaatggaaa gcataggatt
    14941 aaagaaacag acacctttgt ttgattttat aataaccaat aaatcaataa gtatagatga
    15001 aactattaaa aatgtttcat tctctggtgg agtagctgat tatatttact atgatggaga
    15061 aataaaagat tattttgaat atggtgactt gggaatttta cttgggcaag caataaaaaa
    15121 ttgtgaatac cttaaaaaag taaatgtagt taaatctatg gaaacaataa gagccacagt
    15181 ggttggagca ggttctcata ctactgaagt aagtggaagt acaataactt acactagcga
    15241 aaagtttcca cttaagaact tgccagtttt aaaattatca attgatgaag aaacaggtac
    15301 ttcaaaacaa atcagtgaag ctataaaaaa taaacttaat tggtttaaac tagaaaatga
    15361 tttacagaaa gttgctatag ccatgatagg aaagaaaaat ccaacctttt tagatataca
    15421 agagtatgct aaagggtttg tagatggaat gcaacaaatc atagaaaatg atttaccttt
    15481 tatagtaatt gttgaaaatg atatggcaaa agtgttaggt caagcaatat atgcattatt
    15541 aaattatgaa aaagaaattg tttgtataga cagtgtcaag gttgaaaatg gagactatat
    15601 cgacattgga aaacctatag ttgaaggtaa agttttacct gtggtaataa aaacattagt
    15661 ttttaattaa tagaaggagg taagtaaatg attttaaata caaaattatt tggacgtact
    15721 tatcagttta aaggcttata tgatgtaatg gctaaagcta atgaagaaaa atcaggagat
    15781 aaattagcag gtcttgcagc agaatcagct gaagaaagaa tagcagcaaa agttgtttta
    15841 tcacacataa aattggagga tataagaaat aatcctgcag tggcatatga agaagatgaa
    15901 gtaacaagaa taatacaaga tgctgtaaat gaaaaagttt atgaagaaat aaaaaattgg
    15961 acagtctctg aacttagaga atggctattg gattcaaata ctacaagtct tgatataaga
    16021 agaatatcaa gaggattaac ttctgaaatg atagcagcag ttgcaaaatt aatgtcaaat
    16081 atggacttag tttatgcagc taaaaagata aaggttactg ctcactgtaa tacaactata
    16141 ggagaacaag gaacattatc agcaagactt caacctaatc atccaacaga cgacccagat
    16201 ggaatacttg catcacttct tgaaggttta acttttggta taggagatgc agtacttgga
    16261 ttaaatccag ttgatgactc tgttgagagt gttactaaag tattaaaaag atttgaagaa
    16321 gtaaaacaaa aatatcaaat accaactcaa acatgtgtat tggcacacgt tactacacaa
    16381 atggaagcaa taagaaatgg tgctcctgca gatttaatat tccaatctat agcaggttct
    16441 gaaaaaggaa atgaagcatt tggatttaac ggcgacacta tagaagaagc tagacagtta
    16501 gctttaaaac acggtacagc tacaggacct aacgttatgt attttgaaac tggacaaggt
    16561 tcagaattat cttcagaagc acatcatgga atagaccaag ttactatgga agctagatgc
    16621 tatggatttg ctaaaaagta tgaaccattc ttagtaaata cagttgttgg gtttatagga
    16681 cctgagtact tatatgatgc tagagaagtt gtaagagcag gacttgaaga ccactttatg
    16741 ggtaaactac atggcttacc aatgggtgtt gatgtttgtt acactaacca tatgaaggct
    16801 gaccaaaatg atatggaaaa cttagccata ctattaacaa cagcaggatg tacgtatttt
    16861 atgggaatac ctcatggaga tgatgttatg cttaactatc aaacaacagg ataccatgaa
    16921 acagcatcac ttagagaaat gtttggacta acagcaataa aagaatttga tgcttggatg
    16981 gaaaagatgg gattctctaa agatggtaag ttaacagagt tggcgggaga cgcatctgta
    17041 ttattaggat aggaggtatt taatatgaat gagaaggatt taaaagcatt ggtagaacaa
    17101 ttagttggac aaatggttgg agaattagat actaatgttg tgtctgagac tgttaaaaag
    17161 gcaactgaag ttgtagtaga caacaatgct tatatagatg atataacaga agttgatata
    17221 agaaagcaat tactagtaaa aaatcctaag gatgcagaag cttatttaga tatgaaagca
    17281 aaaacacctg caagattagg tataggaaga gctggtacta gatataaaac agaaacagtt
    17341 ttaagattta gagcagacca tgctgctgca caagatgctg tattctctta tgtagatgaa
    17401 gaatttataa aaggaaataa tatgtttgca gtggaaactt tatgtaaaga taaagatgaa
    17461 tacttaacaa gaccagattt gggaagaaaa ttttctccag aaactataaa taatataaaa
    17521 tctaaatttg gtacaaatca aaaggtttta atattagttg gagatgggct tagctcagct
    17581 gcaatagagg caaatttaaa agactgtgtg ccagctataa aacagggtct aaaaatgtat
    17641 ggtatagatt caagtgaaat tttatttgtt aaacattgta gagttggtgc tatggaccac
    17701 ttaggtgaag aattaggttg tgaagttata tgtatgttgg ttggagaaag accaggatta
    17761 gttactgctg aatcaatgtc agcatatata gcttataagc catatatagg aatggcagaa
    17821 gcaaaaagaa cagttatatc aaatattcac aaaggtggaa caactgcagt tgaagcaggt
    17881 gctcatatag ctgaacttat aaaaactatg ttagataaaa aagcttcagg aatagacctt
    17941 aaataaagtc gagggagtgt agaatatgaa aaatgatgta attcgtccta atatactagg
    18001 aattaaaata atttcaaata taagtccaga aatggcacaa aaactagaat taaaatcaca
    18061 tcacaaaagc ttaggtttaa taactgcaga ctgtgatgat gttacgtata cagctcttga
    18121 cgaagcaact aaagcttcag aagttgatgt tgtatatgca aaatcaatgt atgcaggtgc
    18181 tgcaaatgct tcaacaaaac ttgctggtga agtattagga ataatagcag gtccaagccc
    18241 agcagaagta agaagtggtc taaatgcagt agttgatttt ctagagtatg gagcaacttt
    18301 tataagtgca aatgatgatg attcaatagc atactatgct cactgcgtat caagaacagg
    18361 tacatacctt tcagaggtag caggaataag agaaggtgaa gcattagctt atttagtagc
    18421 accacccctt gaagcaatgt atgcactaga tgcagcaatg aaagctgcag atgttaagat
    18481 gtgtgaatta tttgcacctc caactgaaac aaactttggt ggagcattac ttacaggttc
    18541 tcaatcggct tgtaaagcag catgtgatgc atttgcagaa gcagtaaaat cagttgcaga
    18601 caatccaaca ggattttaag gatagttaga tggatgctat aggcttaata gaagtaatag
    18661 gatatgttgc tgcaatagaa gcttctgatg cttgtttaaa atctgcaaat gttaatattg
    18721 taagagttga taaggttggg gcaggtattg ttactttaac aatatgtgga gatgtcggtg
    18781 ctgtaaaagc tgctttagaa gcaggtgaaa tagcagcttc cagagttggt acattaagaa
    18841 catcacatat tataccaaga atccataatg aagtaacaaa tgctttattc aaaactaaag
    18901 aacctaaagt ctgtgaagtt tcagaagata aagaaaaaaa ctttgagatt acattggaaa
    18961 taactgataa tgtatcgaaa aatttatcag aaaaagaaaa tgtaatttta gatgtcaatg
    19021 aaaatagcgg aaatactgaa aatttatata gtgatataga taaaacaaac aaaaaaattg
    19081 aaacagttga tgatagttta acagaagcta atgaggaaaa aatagaaaac actaatgata
    19141 aagaagtatt ggaacaagtt aaaatagaaa attccgattt tgatagagat gacctatcaa
    19201 agaacagtgt aaaaaaactt aaagctatgg ctaagaaatt agattcttca ataacttata
    19261 aagatttaaa tgttcttaaa aaagaagaat taataaactt agttaaaaag cttagcagaa
    19321 gggatagata gttatgacac ttttagataa agatttagta tctatacaag aagtcagaac
    19381 tcttgtaaga agatctaaag tggctcaaga gaaactttct aaaatgagtc aagagcaaat
    19441 agataaaata gtaaaatctg ttgtagatgc agcatttaat aatgctacta aattagctaa
    19501 aatggcaaat gaagaaactg gatttggaaa atgggaagat aaagtaatta aaaatacttt
    19561 tgcatctatg ggagtttatg aaactttaaa gaacacaaaa actattggaa taattggtga
    19621 agataaagaa gccaatacga tagatatagc tacaccagtt ggagttgttg caggtcttat
    19681 accttcaaca aacccaacat caacagtaat atacaaaact ataatatctc taaaagcagg
    19741 aaatactata gttttctctc cacatccaaa tgcaaaaaac tgcatacttg aaacagttaa
    19801 tataataagt gaggctgcta taaaggctgg agctccagag gattcaatat tatgtataac
    19861 taatccaagt atagttggaa ccaatgaatt gatgaaacat aaagatacaa acttaatact
    19921 agcaactgga ggagaagcaa tggttagagc tgcttactct tcaggaacac cagctattgg
    19981 agttggacca ggaaatggac cagcatttat agataaatca gcaaatgtta aattagcaat
    20041 caaaagaata atagattcta aaacttttga taatggtgtt atatgtgcat cagaacaatc
    20101 tatagtaata gaaaaatcta tggaagaaac tgttttaaaa gagcttaaaa atcaaggtgc
    20161 atatgtttta aatgaagagg aaagtgaaag attagcaaaa ttcatattaa gagctaatgg
    20221 tactatgaat ccacaaatag ttggtaaaag tgttgaggta atagcaaatt tagcagactt
    20281 aacaaatgta cctaaaaata caaaagttct agttgcaaga gaaaataaag ttggtcacaa
    20341 tgtagcctat tcaagagaaa aacttacacc aatactagcg ttctatgttg aagaaaatgt
    20401 agaagctatt atgaacagat gcagagaaat attgttaaat gaaggtgcag gtcatacatt
    20461 ctcaatgcat gtaaatgatg aagaattggt aaaaagattt gcccttaata tgccagtatc
    20521 aagaatactt gtgaatactc cttcagcact tggaggaata ggtgcatcaa caaaccttgt
    20581 accagcacta acacttggtt gtggtgcaat aggtggaagt tcaacttcaa acaatatagg
    20641 accacttgat ttaataaatg ttaaaagagt agcttatgga atcaaagaaa tagaagaact
    20701 tggaagagat aaatctcaag ttgaaaaatc aatgtttgac tcaatggata aagaagaatt
    20761 aataaatctt cttgttcaaa aactagtcaa agaactagca taactaaaat aaacaatgaa
    20821 acttatagct atcactataa atttctaaaa atataaacta taaaaaacta taaaattaaa
    20881 tttttggagg ataagattat ggcaagtgca aacgcattag gaatgataga aactaaagga
    20941 ttagtaggag caatagaagc agcagatgca atggttaaag ctgcaaatgt acaattagta
    21001 ggaaaagagc aagtaggtgg aggattagtt actgtaatgg taagaggaga tgttggagct
    21061 gttaaagctg ctacagatgc aggagctgct gctgccgaga gagttggaga actaatatca
    21121 gttcatgtta taccaagacc acactttgaa gtagatgcta tattaccaaa ggtatcagct
    21181 gagtaatata agaataatta taaaatttaa attttgattg aaaaagaggc tatctcaaaa
    21241 atgagtctta ctaataaact caatgtattt tgaggtagtc ttttttatac atctaatagg
    21301 agatgtgata aaatgggtgt tttaacagaa agcgaagtaa aaagaaaaat tggaaaaaac
    21361 aaaaataata taactgaatt ttatatagaa aaaggtcaaa taataactcc ttctgcaaaa
    21421 tcttatttag ctgagagaag tattgagctt aaatatgttg ataaactttc agatgtaaac
    21481 aatgaaccta aagaagttgt aaaagaagta gttaaagaga aaaaatatag atatactact
    21541 gtatttggcg ctaaattgga tgaaaaacca gaacatatga ctcaattaaa tggaaatctt
    21601 ttagttttta aagaccataa aagaataata ttgagaggaa aaatagattc tcttgaatca
    21661 gaaatactaa aggtacagat actttgtcag aaactagata tgccaaagct tgttaaggaa
    21721 ttgcaagaag tattagactt tgtaagaaat ataatgagat gtgaagtttt agatgaaaag
    21781 ctaggggact ttaaactttt aggattaact cctcaagaac ttagagaaca atctcactat
    21841 cctaaaaaat actttggtat aggacatgag ttcccaagtt atgaaatggg agaagtagta
    21901 gtagctataa atagtataag aagtaataca cgagaggttg aacttgcagc ctatgaagca
    21961 tttaagggtg agtatggaaa tgttcacaga gaagatttaa taaaggcact taatagatta
    22021 tcgtcagtat tttggataat gatatataag gttagaacta ataagtataa gtagttggag
    22081 gacataatgg agaatctatt aaatgaaata gtggaagaag taataactag agttaaaaaa
    22141 gaagcattta tagaagttga agcatcagga agacacgttc acttaagtag agaagatgtc
    22201 gataaattat ttggagaagg atatactctt acaaaactaa aagacctatc tcaaccaggt
    22261 caatatgcat gtaaggaaag agttacaata actggtccta aaggaagtat aaaaaatgta
    22321 gttgtacttg gaccatgtag aaatgaaact caggtagaaa tatctttaac agatggttca
    22381 actcttggac taaaagctcc tataaaacaa agtggagact tagaaggtac tttaagtatt
    22441 aaaatatcaa cacaatatgg tgaagttgaa cttgataagg ggttaatggt tgctaaaaga
    22501 catatacaca tgactcctaa agatgctgaa aaattcaatg tatgtgataa agagatagtt
    22561 gaagctaagg tgatgggtca aagaccttta acatttgatg atgtagtaat aagagtgagt
    22621 gacagtttta aaacttatat gcatatagac tatgatgaag ccaatgcatg tggttattca
    22681 aaaggaactg ttgctaaaat aataaagaaa gcgtaaattt aacctactat ataaattacc
    22741 tctagggagg atttaatatg aattatgata accttgtaga tttaataacg gaagaaatct
    22801 ataaaaaaat aaatagtaat gaaataaaaa tatctaataa acctaaagct gtgattgtat
    22861 ttgaacaaga taacaataaa tttaacttat taaaagatga atttgaaata gtagaatttg
    22921 ataaaagtat aagggaatgt gaaatagtga tagtttcaag actttgtatg agggggctat
    22981 cgaatatagc acttggaaat tcaacaagtg atgaagaaag atttatacta aaaatgatta
    23041 tgaagggcaa agaagtatat gtcctagatg aaggaattga atacaaaaaa tataaggata
    23101 ctgcaccaaa agcattatat aagaaattta tgtcatttga agatgaaata tgtaaatttg
    23161 gtgtagaaat tataaaagac ttaaatgtta taactaaaaa taagttaaat ataaagaaag
    23221 aactagaaag tactagaggt tctaaaaaag aagatacaaa agttttagat aaagatgttt
    23281 taaatttaat cagtgtagaa agtgtaaaaa tagataatga atttagttta gatttaagga
    23341 ataaaaaact tatatcggaa gctgacttaa gaaaaccaac tataaatgga gttaaaaaca
    23401 tattagttaa taaaaaaagt ataataacac cattagctgt agattttatg agaatacacc
    23461 atctcaaact aaagaaagtg taaggaggta attttatgga tataggaaaa gttgtaggta
    23521 atgtttgggc tacaagaaaa gatgaaaaat tatgtggcca aaaactttta gttgtaaaaa
    23581 ttttaaaaac taaagacaca tacaaggaag gcttcatggt tgcagccgat aatgcaggag
    23641 caggaaatgg agacttagtt cttatatcca aaggttcctc agctagagag tcgattgaaa
    23701 aatctcatgt tccggtagat gcaactatag ttggagtagt cgattcgttt gaggtgtttg
    23761 atgaataaaa gtataggagc tatagagttt aaaagcattg ctaaaggtat agaagtatcc
    23821 aatgaaatga taaagaaatc ttcagttgat gtattgtact taaaaagtat ttgcccaggt
    23881 aaatttctaa taatagtagg tggagaaact tcttatataa atgaatgtgt agattatgga
    23941 ataaaacttg gagaaggata tattgtagat aacttcgtaa taaatgcaat ttcacaagaa
    24001 atattagatg gatttaaaaa taagtatcaa aagctagata gtatagtctc tataggcgtt
    24061 gttgaaagta gtaaagtatg cgctggaata aaaatgcttg ataagacttt aaaatctggc
    24121 gacttagtat tagtaaaact tcaattatct tttgctatag gtggcaaatt agtatatata
    24181 gtagcaggag atttaagtag tttagaatat gcattaaaag aaagtgaaaa tgtagtaaga
    24241 gaaaaagaag taatatataa gactgtaata ccatcagtag acagtcaaat aataaaaagt
    24301 ctaataaaat aatgggggga attatgagta taaacgaaat tataatctat gtaatggtag
    24361 tcttcatggt acttggagct atagataaat gtataggtaa caaatttggt cttggagaac
    24421 aatttgaaga aggtataatg gcaatggggt cacttgcagt tgcaatggtt ggtgttatat
    24481 gtttagcacc agttcttgct gatgtattaa gaccaattgt agtaccagta tttaatatac
    24541 ttggagctga cccagctatg tttgctggaa gtttattagc taatgatatg ggtggtgcac
    24601 cacttgcact tgaacttgcg aaagacccta atgctgggtt atttggtgga ttaatagtag
    24661 gggctatgat gggacctaca atagtattta taataccagt tgcgcttgga ataattgaaa
    24721 aagaagacca aaaattctta gcaacaggaa tacttgcagg tataataaca atacctattg
    24781 gagcattcgt tggtggatta gttgcaggat tcgaggtaat gatggttctt agaaacttag
    24841 tgccaataat aatatttgca gtagttatag cacttggatt attaaaattc gaaaatgcta
    24901 tgataaaagg attcacatac tttggaaaag gtgtagtaat agttataact ttaggacttg
    24961 ctgctgccat agttgaagct ttaacaggaa tagttgtaat acctggaatg aaaccaatca
    25021 gtgaaggtat agaaatagtt ggagatatag caatagtttt agcaggagca ttcccattag
    25081 tgtttgttat aacaaaggta tttaataaac cattaatggc tttaggaaaa gttcttggaa
    25141 tgaatgatgt atcagcagca ggccttgttg caagtttagc taactgtata ccaatgtttg
    25201 gtatgatgaa agatatggac aatagaggaa aaataataaa tgtagcattc tctgtatcag
    25261 cagcattcgt atttggagac cacttaggat ttacggcagg atttaattct aatatgataa
    25321 caccaatgat agttgctaaa ttagttggtg gtataacagc agtattgtta gctatggtta
    25381 tcgcaaataa gacactaaag aaggagaatg tataagatgg atatatcaaa tatagataaa
    25441 aatttaattg aaacattagt aagacaaata atagaagaaa aaataagtgg aactaaagac
    25501 acagtagatt ttgttagaaa taaagacatt agtggaataa cttctataaa gttaccaact
    25561 gtaaaagtta gtgaatctga tagattagat acagggaatc caagtgatgt agtttacaca
    25621 aaagatttat ttacattaga ggaaagccca agattaggtt gtggtatgat ggaaatgaaa
    25681 gaaacaactt ttgattggac tctaaactat gatgaaatag attatgtaat agatggaact
    25741 ttagatataa taatagatgg aagaaaggtt tctgcttctt caggtgaatt aatatttata
    25801 ccaaaaggaa gtaagataca attttctgta cctgattatg ctagatttat atatgtaaca
    25861 tatccggcag attgggcatc tcaaaattaa taaaaatcaa gttaagttaa taataatcaa
    25921 ataaataatt taaaatacaa actcttaaat aagcacacat atttaaaaaa gctatgaatt
    25981 aaaattcata gcttttttta taaagaatgt tatattttag acgtattttt tctatattta
    26041 ctgggagaca tattatattt tttattaaat aatctaataa aatgctctac atttggataa
    26101 ccaatactat tttactgata atcttttcct tatcactaat cttacatttt aaaatattat
    26161 ttttactcgt aatattatca taagaatttg tttcttattt aaaactaatt agaattataa
    26221 agtttattgc catgttatta ataaaataaa tccagttaaa tcgagtgcta gttaaagtaa
    26281 taagaaactt ttacaatggt ttcctttatc gattgaaata aaagttaata tggtataata
    26341 tctaaagaga gaatcttatc aattagaata tgcatagaaa caagtggctt aaaaggagac
    26401 gttatatgga agagatagta aagaaggaac aagcaagtat gaatgatatt tttttgtatc
    26461 ttaaagctaa atcaaactca ttatataaat ttgtaatatt atataatgag tttatcaata
    26521 aacctaaaga ttatggaact ggggaactta taaacatggt tgaagtacat atgctaacag
    26581 cgattgaaga aaatccagga ataagtccaa ctcagttagc taaaatatgg aacagaacta
    26641 aaggtgcaat atctcaaact atatctaaat tggagaaaaa gggttatata actaaagaaa
    26701 agaaagaagg aaatgcaaaa actatattac tatatccaac cacatcagga gtagagttaa
    26761 gtaaggcaca taaactatat gacaccatag atgttacaag aacatttgaa tacttaatca
    26821 aggagtgtac attagaggag atagatagtt tttataaagt aattgatttg tttattaatt
    26881 tattagaaga atctccgcag gaaaataatg attctgaaaa agtgtagctg agattgaagt
    26941 tgaataaaga gaaatagata ttatagaagt agtacttaag tacacattat ataaaaatat
    27001 aataggtata ggattaaaaa gtttagtggt ttaaagttta accactaaac ttttttaatg
    27061 tctataatct actattattt aatttactaa aaataaaaaa actcattata ttaagataaa
    27121 aatagaaaaa taactagaaa aatactataa tatatcagct taatacagaa aaaagttaaa
    27181 aaatttgaga aaaataaaaa tgaaaacgtt ttatattgac atagagaaaa agtagtgata
    27241 ccatttaaat atagtttagt agctaaacta tatttaaatt ataataacta ttttaggggg
    27301 atgttatgaa tttaaaacaa ttaagtgaaa aatacgaaga atatgtaatt caaatgagaa
    27361 gagaatttca taagtatcca gaattatctt ttaaagaaat aagaacaacc caaagaataa
    27421 gggaagaatt acaaaaaata ggggtccctt ttgtatctat tgaaccaaat atcgtaatag
    27481 ctactataca aggcaaaaat gaaggtaaga caattgcaat tagaggagat atagatgcgc
    27541 ttccaatgca agaagattca gatgttgaat acaaatcaga atatgatgga ataatgcatt
    27601 cttgtggaca tgatgctcat ggagctatgt tactaggact tgcacatatg ctaaatgata
    27661 taagaaaaga tttaaaaggt agagtattat tagtttttca agcagctgaa gaagttggtg
    27721 gtggacacaa ggagataata cagtacttta aggaaaatgg aggaccagat agattaatag
    27781 ctactcatgt ttggtccgat attgaagctg gtaaaatttc tgttgaacca ggacctcgta
    27841 tggctggtgg gtctggttgg agaatagaca taacaggtag aggaggtcat ggttctagac
    27901 cagatcaaag catagatcct ataaagccag cttgtgatat agttttaaaa gtatcttcta
    27961 taccagttaa tcatataagt gtactagaac aatgtgttgt acatgcatgt gcaatacatg
    28021 gaggaactac aataggaaat gttattccaa acaatgcaga ggtaataggt ggatatagat
    28081 attttactga agaatcaggc aaaaaagtgt ttgacattat aaagcaaatg agtaatggtg
    28141 ttggacaagt ttatggagtg gatgtgaagg ttactcatac tggaggcata ggacctgtta
    28201 tcaatgatga agaagcagtt aagatggcta aagaaatagt ttctaatata gatggattgg
    28261 agttagattc ttatgagcca atatgtgcat cagattgtta tggtgaaata ttaaaagagt
    28321 attctggctt ttattgttat ctaggggtag gtaacaaaga aaaaggaata atatatgcac
    28381 aacaccatcc taagtacaat atagatgaag atacattaaa gttaggttgc gaatttatgg
    28441 ctaaatatgc agttgaattt ttaaataaaa ataataacta aaataaatcg aattttataa
    28501 atggggggga tgaaaatgca aggtgcatta aaggaaaata agaaaaaaag tggtggagga
    28561 tttttaggat ggattgaaaa agttgggaat aaaatgccac atcctctagc attatttaca
    28621 tatattacat tgatagtagt agcattatct gcaatagcaa gctttttagg tttttcagca
    28681 acaagtcctg ctacaggaaa gttaataaaa gttatgaact taataagtgc aaaaggttta
    28741 gtactattta tgcaagagtt tgttaagaac ttccaaaact tccctgtgct tggagtagtt
    28801 ttagttatgg cagttgccac aggagtttgt gaaaagacag gatttttttc aactgccata
    28861 aaaatgagtt tatcaaaagt taaggggaat gcggttgtat ttataatagc ttttatagga
    28921 gtatttgcta atcaagctgg tgatgctgct tttgtattag taccaactct tgcaggagca
    28981 atattttatg gattaaatag aaatccatta gctggtatat tctgtggatt tgcatcagtt
    29041 ggtggtggat ttgcaacagc tgtaattcct gggggatggg acgttacgct aacgccaata
    29101 actgtatcag ctgctcaaac tattcaacct gcatttgata tgacattatt agctagttat
    29161 tatgcactat ttgtatctgc gtttatagta gctacagtat caacaatagt aacggttaaa
    29221 tttatagaac ctaaattagg taagtatact ggtaaaccag aaggtataga tgataatcaa
    29281 ggttttgagg tcacaaaaga acaagcaaaa gcagcaaagt tagcaggttt tacagtttta
    29341 gcttatgtag cgttattaat tgctttgtgt ataccagcta atagtttttt aagaagtcca
    29401 gaaggttctt taatatttgg agcaccactt atgtcttgct tacagttttt tatagtaata
    29461 ctattcttct tgccagggat tgtgtatgga atgagggttg gtaaaatcaa gactgtaaaa
    29521 gacttaaatg atatattatg tcaatcaatg gcagcaatgg caccgtttat cgtacttgca
    29581 atagttatag gtcaattctt aacgctattc tcagtgtcta atttagcaca ggtactagct
    29641 ataaaaggtg gagcattact caactcattg ccactaccac cacaagctat aatactgcta
    29701 ttcttagtac tagtagcgtt tataaatata tttgttataa gcggaagtac aaaatggatg
    29761 atatttggac cagtatttgt tccaatgtta atgcaattaa acatacatcc agcatttact
    29821 cagtttgtat atagattagg agattctgta accaatcatt tatcaccatt aaatgcattc
    29881 tttataatac ttttagcaac agtccaaaaa tacgataaaa atgcaggtat gggaactata
    29941 ttctcagcta tgatacctta cactataggt tactttgtag tacttgcagt tttagtagta
    30001 gtttggatga caataggtat accagtagga ataggtggac aagtttggtt ataaaacata
    30061 tttaataatt ttgggaggaa agaaaatgga aacaactgaa ataagataca attttgatga
    30121 agtaatagat agaagtggaa caaactgtct taaatatgat cttaagagtc aatttatgcc
    30181 agaatctcca gaagatgcac taccactatg ggtagcagat atggattttg catgtgcccc
    30241 tgagataatt aatgcaatgc atgaaagaat agacagaaaa atatttggat atagctctca
    30301 acaaactgat gattattatg aagctgtatg tggatggttt aagagaagat ttgattggaa
    30361 aatagaaaaa gagaatatat tctttagccc aggggttgtg cctgcgttag gatttttcac
    30421 acaaatatta acacaaaagg gtgatggtat cataatagaa aagcctgtat actatccttt
    30481 tgaagataaa ataactaata atgaaagaaa agtagttaat agtcctttaa agtttgaaga
    30541 tggtcattat tctatggatt atgatggatt tgaagaatta gctaaagatg aaaataataa
    30601 ggtgtttata ttatgtagtc ctcataatcc agttggtaga gtatggaaaa aagaagagct
    30661 tagaaaaata gtagacatat gtaagaaata tgatttgtgg ataatatctg atgaaataca
    30721 ttgtgatatt attcgtaaag gtgaaaagca tataccatta gaagtagcat gtcctgatta
    30781 taaagataga ataataactt gtacagctcc aagtaagagt tttaacttag ctggtatgca
    30841 attatcaaat atagtaatca ataataagga attacaaaaa aaatggatgc ttgaaacaga
    30901 ttctaaatta gctatattcc tacctaatcc attcgcaata gttgctacta aagttgctta
    30961 taatgattgt gaagattgga tgaatcaagt aaatgagtat atagatggta atattaaata
    31021 tgtagaagat tttgttaatg agtatatgcc taaagtaaag ataataaaat ctcaaggaac
    31081 ttacttagtt tggttagact ttagagggta tggattagat gcaaaacagc ttgaatatgt
    31141 aatgcttaac aaggcgaagg tattgctaga tgaaggatac atattcggag aagaaggagc
    31201 aggatttgaa agaataaatg ttgcttcacc tagaacaatt atagaagagt gtatgaatag
    31261 aataaaatgt gcatttgaag gattataata gtaatatcta aataatatat ttgtgtattt
    31321 taaaaaaaga ctatgctaaa aaaatatttc aaagcatagt cttttaaagt tttatgatga
    31381 gtttaaaaaa tttcaaaacg ttactattca cttatatgtt tatattagaa ataattccac
    31441 aatatactca ttttctaaag ttgcactttt atacaccttg cttcaccaat atcatgtgta
    31501 accaaaatag tagacttatt ctcattctta ataatactat aaacatcatc acaaactaat
    31561 aatctagtct gataatcaag agctgaaaaa ggttcatcta aaagtagaat atctggattt
    31621 acagaaagtg ttctaataag cgcaactctt tgtctcatac ctccagataa ttcttttgga
    31681 tacatacttc taaaatccca taatccataa gtctttaaaa ggctttcaac tctatctaaa
    31741 gattttttat cttttttgcc ttgaatttcc aatccaatag ttatattatc cattatattt
    31801 ctccattcaa gcaaattatc tttttgaaac atatatccaa tatttccagt aatctttata
    31861 tcaccactag tcggttttaa aagattagta agaatattta atattgtaga tttaccactt
    31921 ccagaaggac caagcaaagt taatatttct ccttctttca agttaaaatt gacatccttt
    31981 aatacttcaa gctcgccttc cttagtgtag aagttcatat ttatattttt aacttctact
    32041 atgttgtcat tcattatgcc accccctata acttttagct cgtatatatt atgttattaa
    32101 caatatgacc tatttgtacc atttttcata aatataatat aaatattttg tttatttacg
    32161 tattttctta gtaacttgta cgtataaatt atagataaat aaattataag gtaggggata
    32221 aatttgagta acataataac gaatttaata gagtcaatat ttactaatcc aataactata
    32281 ttgtttttac ttgcaataac tgtgtattct gtttttaaaa taatagaaaa taacagagta
    32341 tattcatttg taagttcaaa attagacgaa atcaatagag actataagac aagtgagttt
    32401 tataaaaagg taagggatga ctactataca tatagtaaag aaaatcctta tgcagacgta
    32461 aatataactt catttataga ggaagttgta tctgatttaa agcataataa tttgccattg
    32521 ttagaaaaaa ttagaagtat aaaaaattca tcttcaataa gtatattact tggggtttta
    32581 ggtacgtttg ttggtctttc tacaatgctt ttatgtgttg atactaaaga cattataaat
    32641 tcacttcctt caactattag tagtatgcaa actgcattta caactagtat atttggagtt
    32701 gtattttctc taattatagg ttactttact aagataaaag attgtgaaca tgttttgata
    32761 caaataatgt taaaatctga aaatttacta acatctgaaa taactcattt taagtcagaa
    32821 agaatggatt taaaagtaga agaggtaaaa aatacaataa aacaaataag taagtctata
    32881 gaagctattg aaaaatttga taaaatatct aaagatttaa atgactttaa tgatgaattt
    32941 atcagtggta tagaagcttt aaaaagcctt ttagaaggtt ctcaaagttc tattaagaca
    33001 tttgaccaga gcgtaagaaa attagataaa cagtttaata ttttaaatat aaaatttgta
    33061 aagctttttg ataaatatga taatcaagat aatataaata aagagatttt atttgatata
    33121 aaagaatctt caaaaaatat atacaatgcc actgaaagcc aattcaaaat tagagattat
    33181 ataaaaaata taaatgcagg atttgctcta tatgaaagaa gtgctcaaga tttattaact
    33241 aaattaatga cccatgaaaa taaaatatct caaaatcaaa aaatcctttt agatgaaaag
    33301 tttaccttag atgactcaat aaaaaatctc tcaagtatta ttgaaaactt ttctaatgac
    33361 ctacaagtta aactggatat gatgtttgaa aattctttag atattcaaga taagttagat
    33421 gttatgttta ataactcttt tatgaatgat gaagttcctc ttgatagtga agaattattt
    33481 aaggatgatg ttaataatgt atttaatcct tttagtgaag aaatttatga gatagaggat
    33541 aaagaaataa aggtcatagg tgaggatgag ttaaatgaat aataaatata gaagaactgt
    33601 taacagagaa tttgaaaaga gtagcttttg gccaacattt acagatttat tatctactgt
    33661 attgatggtt gtgattctta tcttatttag ctcagaaagt atttcaggtt ctgttgaaca
    33721 agatttagct aagaatgtca atgcttctgt tgaagaaact tttaaaaaaa gtggaatacc
    33781 tgtgaaggta gataaatcta atgggcaagt aacttttggt gaaaaaacca tgtttgatgt
    33841 agatagtgat gttttaaaac cagaggctaa agaaatgcta aaaatgtttg ttccaaagta
    33901 catagagact atttataaag attatggtga ctatatatcc aaaatagtta tagaaggtca
    33961 tacagatgat gtgggaagtt atatatacaa cttagattta tcgcaaagaa gagcatatag
    34021 cgttgctaaa tttatagtgg gagatgaaat aggtgactat aaatacaaag ataaagtgac
    34081 taagcatatt attgcaatag gaaggtcaaa agctgaactc atcaaaaatg gtgacaatag
    34141 cgtgaataga gatgcatcta gaagagtaga actaaaatac gaaataaata ttaatcaaaa
    34201 taaataatta aatttttata aaaaaaaaga aggcagttgg tgtgagcttt aagccttctt
    34261 taaggaaaaa gtttatttat ttttgttgat acttaacata ttatagtttt tagaagcagt
    34321 ttgcaatata ttaaacgtaa atggtcgttt aaaatgcata actggatact actgatgtaa
    34381 aatatttcat tttatataat ataaaaatta tacagtgtca attttctata ttattgtact
    34441 ataatctatt atttaaaata ttatgtaaaa aaatatcata aaaacgcata tcatgttata
    34501 attactagag tattatgaat atggaggcgt agtttgtgat agaaataagt ataaaaaata
    34561 aaaaaaattc taaaattaaa actttgatac ttttattgat agtatctata tcgatttatt
    34621 taactggttg tactacatca gataatggaa ataatcaatc aaataataat gattctttgt
    34681 ctagtagtat atcaagtgat aagaatgtaa aaattcattt tattgacact ggaaatagtg
    34741 atgcaatatt gattcaagat ggtaaaactt ttacattgat agatggggga gataatgatg
    34801 atgagggatt aatggtagat tacctaaaca atcaaggtgt aaaagatatt aaatatctta
    34861 tagctaccca ttctcatgca gaccatttag gtggacttga ttctgttgtc aaaaacttta
    34921 atatagaaaa tgtttttgta tcgaatggtt cagcagaaac aaagagttat agggacttta
    34981 taaatgcttt agcaaataag ggtttatccc caagtgtacc gcttgaaaat aataagttct
    35041 atttagaaga ttcttatttt gaagttctaa atacaaatgg gggagatact actaatgaac
    35101 aatctctagt ccttgtatat actaatggga atgataaagt tttatttaca ggtgatgctg
    35161 aagaaggtac cgaaaaagaa atactaccta agcttgagaa agtagacctt ttaaaagtag
    35221 ctcatcatgg ttctcgtagt agttcatctc aagagttttt agataaagta aatcctgaat
    35281 atgcggttct tttagtaggc aagggtaatt catatggtca tccacatcag gagacaatga
    35341 ataaattaaa aaaaatggga gtaaaagtgc atagaagtga tgagtgttca gatataatat
    35401 ttgaatcaac aggtgatgga gtatttacaa gttgtaaaga tggaagttat aataaaggcg
    35461 ttagagaaga tggaaactct ggaaaatcaa acagtaatac aagtaaaaat gattctttta
    35521 aaaataaaca aaatacatcc aattcagaag aaatagtgta ttttactcca aaaggtaaga
    35581 gctatcactc tacaaaaaat tgtagtgggc tttcaagaag taaaaaaata ttatcaggca
    35641 caattgcaga aagtaagaaa aatgacccat gtgatatatg ttatggaaaa taacaaaaaa
    35701 acaaccaatt tatctataaa gcttctgaac tttaaatcat tcaaaaaata aaataaatgc
    35761 ataaaaaaat aagctataat caatagataa cacattttat ctatacgaat agcttatttt
    35821 tttaagtatt ttttaatagc tatttaataa ctctaaatac accagttaca atacctacaa
    35881 tttcaacttc tgagtcattg agcataattg gttccataaa atcattttct ggctgaagtc
    35941 ttattaaatt tccttcttta aaaaacctct taactgtagc agattcacca tttataagtg
    36001 caactactat ctgagaattt aaggctgtat tttttttgtc tactacaacg taatcttcat
    36061 ctaaaattcc tgcattaatc atactttcgc cttttactct taatacaaaa ttgttactgc
    36121 cctttactaa actggcaggg aatggaatgt attcctctat gttttgttga gctaaaatgg
    36181 gttctccagc tgttatttga cctactaaag gaagctctat tatttcttga ttagctccag
    36241 aaacatcatt tactttacta cgttctaaaa cttcaatagc tcttggttta gtagggtcct
    36301 ttcttatgta tcccagtttt tcaagtttat ttagatgaga gtgcacagtt gaagtagatc
    36361 ttaaccctac agcagtacat atttctctta cagcaggtgg ataacctttt aatatgattt
    36421 gagactttat aaattccaat atcaagactt gcttttcagt tagatctaaa tacataacat
    36481 cactcatttc caaacatatt ttgtttttat ttccatacac tttatatttt taattataac
    36541 ataatccatt tatatctgca aacatttgtt cctgattttt aatcgtcctc aatccattca
    36601 aaattcttga ctatatcacg ttttacattt ttcttatgag ctgcataaag ctgagtagtt
    36661 gtgacattat catggtcaag taagttttga acatcatata tagaaaatcc tgtttggatt
    36721 agagaagttg cagccgttgc tcttaattta tggggactat atccattgtc tctagtagta
    36781 tcaagtggaa tagatgtata tttttttacc agtgttctta tagcctttgc tgtaattctc
    36841 ttattttgaa gagacaaaaa taaagcatct ttttcttcat catttaatag ttcatctctt
    36901 gttctttcgt tttgcaaata atcttttatt acatgttcac aagtatgatt tataggcatt
    36961 aaaacttctt tcccacgttt tctatatatt ttaaattcac ctcttgaaaa attaaaagag
    37021 gagatattga gttctctaag ctcatttaat ctaagcccgt atgtaacaaa taatgctagt
    37081 atagctttgt cacgtaattt agtttttctc caatatacct tttctttctc tgtaagccct
    37141 tcacctgttt ctactgattc aagcatctta gcaacttcat ctatctcaag gcgcttaata
    37201 gcatctggct gaggtttagg aagttttatt ggattaaatc catcagttat attattatct
    37261 atctgagagt ttctgtataa gaatttaaat agagtagaga tggatgattt ttttcttgct
    37321 aaagctctat tgttattttc aaaaatcaat gtgttttttt ctgtattttt gtaatatcta
    37381 ctgcaataat cacctaagaa taaatttata tctcttgatt ttattgtatt aaaatcatct
    37441 tctgtaatat cttttacaca atctgcattt gttaattctt tggtttcaat caaatatgaa
    37501 caaaaaaaat taatatcttc taaataagca agtcgtgtag aaactgcaac agaacctttt
    37561 aaatatataa aataatcctt cataaataca ggaagtttat tttccaaaag tagacatttt
    37621 tctatgagtt cattttcttt gataacaata ttatcaggtt tatctgattt attatgtact
    37681 tttataattt tcttatcatt attcaaaatt aaacctccta aaactaaaat ataaatcttt
    37741 tatattattt tttgatattt ataccaagtg agtataaata tttaatttaa tcatttttta
    37801 catttttaag agattagcag tactttagat gaaattatca ctaactattc cctaaatcca
    37861 catttaaaga ctagttgaat ttaattactc tttttttatt tctcaatata tttatagaaa
    37921 tattttaaaa tatattaata tttttatatg tttctaacga aaccaaatat tagtaaaata
    37981 tgtatttatt taactaaagt ttttaaatgg gctgactaag cttaaaatat aggatattat
    38041 gagagttact attgaaaaaa taaaccctat tgccataaag aaacttataa atatgttata
    38101 cttaactatt aaactggata tattcaagtt atcaaatcca aataagtatg taattgaact
    38161 tattacaaaa ataactataa ctataaagct cagaccattt aagctatttt ttaaatcagc
    38221 actactaagt gacatatgtg tagatataga atacattaaa aacataaata taaaatactt
    38281 caatgaatat acactaggtg ttaacaacat tattcttata aaattggaaa tatcacctaa
    38341 aataatgtgt ataaaaccat ttatatttaa gttattaatc atacttacat ataactttaa
    38401 atctatattt tgagtcactt tcatataact atctggcagt aatatcttaa aaattaaaat
    38461 aatcattatt gttccaccta taataggtgc aattcctata aagaaatttc ctatactttg
    38521 ataaagatta tcttttttat agctatgact tacatatcct agtataccat catttttact
    38581 ttctattggt ctgaaaaact taactgaatt tattttatgc atgaaaatta aacacattat
    38641 caaatgactc aattcatgaa caaatgttcc tatagctcca gttacaataa ttccagtttt
    38701 tccaaaagat gaaaatataa atttattatt tagattttct gtaatgctaa aaatcaatcc
    38761 aaatacaata aatattccta tgaaacaaaa aatatttatt acacttattt ttaaaatttc
    38821 gattatataa atcataagtt aagtcctcat gtaaaagtat ttaactactc tacttcatat
    38881 ccattacagc ttaaaatttt acaagcattt tctatatctt tttcttttac taagatatag
    38941 tctgtatcat acgtagaaat tgcaaatatg cttattttgt tgtctgctaa caaactactt
    39001 aacttagata aaattcctac aagtgaaaag tctaaaggac ctagtatctt aagtattctc
    39061 cactcctttt ctgacttgac atcagaagga atattattat ttaaacatac aacacttaat
    39121 tcatcatcag ttctagttat agagtaaaac ttctctgtat ctatccatgt tggtatttta
    39181 ctgtcattat ttaatctaca tactgcatac tcttctacca ataattttaa tttcataatg
    39241 tatatttcct ccaatcttat ttgtttactt aagattatac attaaaagtt acataaaata
    39301 aaacatataa agtaaaaaag gttcactata gtgtcctatt tctatagcca acctttaaaa
    39361 cttaattaaa ttctaataaa gagatatatt atctaggttc aattataagt ttaatggctg
    39421 ttttcttatc accttcgatt tctatatcag taaatgcggg aatgcaaact aaatccattc
    39481 cactaggtgc aacaaaacct cttgctattg caattgattt tatcgcttga tttagagctc
    39541 cagctcctat agcttgtatt tctgcaacgc ctcgttctct tagtactcca gccaaggctc
    39601 cagctacaga attaggatta gattttgatg aaacttttag tacctccatt tcaacatctc
    39661 cttgttaatt tgttaaagta tccattacct aaagtactaa atcataattg ccatttacta
    39721 attacaatca tcatttaaag tatactttta taaaaatata cttctataat cgttttaaga
    39781 aaacctttat tttttttaac cattttatta tgaaaaaact acaaatttct ttatattcta
    39841 taagcatttt ccaatatttc taaatttttt atatctttga gctataattt cctctttatc
    39901 catatctttg taatgattta atctttctaa aatataagct tttatttctt ttgaaacagc
    39961 ttctacatct ttttgtgctc ctccaagagg ttcttttata atcttatcta ttatttttaa
    40021 atcatataaa tcattacttg taagcttcat aacatcacaa gcttctttag ctttagatga
    40081 atctttaaat agaatactag aaagcccttc tggagaaatt acagaataaa ctgaatgttc
    40141 taacatacat acatcatttc ctactccgat acctaaagct cctccactac caccttcacc
    40201 aattacaaaa gtgattactg gaacagaaag tttactcatt tcaagtaagt ttcttgcaat
    40261 agcttctcct tgtcctcttt cttctgcttc tagaccacaa aaagctcctg atgtatcaat
    40321 aaacgttatt ataggtctat taaatttttc agcttgtttc ataagtctta atgcttttct
    40381 gtatccttct ggatgaggca ttccaaaatt tctttttata ttttcttttg tatctcttcc
    40441 tttttggtgt ccaactatag taacattaaa ctttcctatc tgaccaattc ctccaaccat
    40501 agaagggtca tctttaaaaa gcctatctcc atgaagttct aaaaaattag aacaaatatg
    40561 ttctatgtat tccaatgtcg taggtctctt tatatctcta cttagtgtta ccttttcata
    40621 tgctgataga tgtgaaaaag catcttcctt aagcttttcc aatttctcat taagtgaatt
    40681 tattttatca cttaaatcta tattatttgc cttagaaata gttattaatt gtttaatatc
    40741 attttctaag atttttattt tttcactatt attttcaatc aaaacagttc ctcctttatg
    40801 ccctatttct tgtgcatagc aagaatttga tataatactt ccttcatttt tcttctatct
    40861 actatcatgt caataaaacc tttttcaagt aaaaactcag aagtttgaaa tccttcagga
    40921 agtttttgat ttattgtttg ctctattact cttggacctg caaaacctat caacgtattt
    40981 ggctcagaaa ttataatatc accaagcata gcaaaactag cagttactcc tccagtagta
    41041 gggtcagtta gaactgatat atatggcaat gatttttctt caagcttaga aagtgcttga
    41101 gaagtctttg ccatctgcat aagagatacc ataccttctt gcattctagc tcctcctgaa
    41161 gcagaacaaa taattatagg taaattattt tcagcagcat actctattga ataagttatt
    41221 ttttccccaa caatagaacc catacttccc atcataaaat caggattcat tacacaaata
    41281 actgttttaa ttccatttat tcttccataa cctgtgacaa cagcttctga ctcttttgtt
    41341 ttttccttat aagagtctac tttttctttg tatttaggaa aatttagtgg attttgaaat
    41401 tctacatcag aattaaactc cataaaagtt cctttatcaa tcaataaatt tattcttttt
    41461 cttgctctta agccataatg tcttgaacat tttggacata caaatgaatt ttcttctatg
    41521 tcctttctaa atacatgact atcacaacct ttgcaatagg tccaaaattt atcatctaca
    41581 ctattctttt taaagttgtc atctaaagaa atcgtaacat acttagattc ttttgatgat
    41641 aaaaattttt ttatcataat ctatttactc cttataatgt agcaaattct ttcttcataa
    41701 agacatcttc tataaatttg gtatgatgag tatcttctct aaactcctca cttgttacaa
    41761 gtgccttttg aaattctaca tttgtattaa taccttctac aataatttcg tctagagctc
    41821 tatatattct acttatacat tcttctctag tttcagccca acatattaac ttacctatca
    41881 tagaatcata taaaggtggt atagtatatc ctgcatatac aaaagtatca aatctaacac
    41941 caaaacctcc aggtaaattt aatgattcta tcttacctgg tgatggagca aaattctttg
    42001 atgaatcttc tgcatttatt ctacattcaa tagcatgacc tctaaaagta atatcttctt
    42061 gtttaaaagg aattttatcc ccattagcta tagatatttg aagttttata aggtcaagat
    42121 ttgttaccat ctctgtaact ggatgttcaa cttgtattct tgtattcatc tctataaaat
    42181 aaaagttttt atctttgtca actataaact caatagtacc tgcaccgata taacctactc
    42241 cttttactat atctactgca attttatgaa gtttttgtct ttcttcatct gaaaggtatg
    42301 gacttaaaga ttcttctatt actttttgat ttcgcctttg catagaacaa tctctatcac
    42361 ctaaatgtat tgcatttgaa tgtttatctc caaatacttg tacttctata tgtctaggat
    42421 tttctataaa cttttccata tataaatcac tattagaaaa tgctgctaga gcttctgaac
    42481 ttgccatatc aaaatttgaa aaaagttctt cttcttttct aacgattctc ataccttttc
    42541 caccgcctcc acttgaggct tttatcataa caggatatcc aatttctctt gcaacttcta
    42601 aagcttcttc cttagttttc aaaacagatt ctgaaccagg aacaacaggg atattcaatt
    42661 ccatcatagt ttctcttgct ctagatttat ctcccatcaa aatcatttgt tctcttgttg
    42721 gacctataaa aataatatta ttatcttcac attgctttgc aaattcagga ttctctgata
    42781 aaaatccaaa gcctggatga atagcatcac actttaattt aagagcaaga tagattatat
    42841 tttctatatt attataactc ttacttatat tatttggtcc tatacaaata gattcatcag
    42901 ctatatatct atgaaaacaa tctttatcta tttcagaata gatagcaaca gtttttatac
    42961 ctagttcttt acatgttctg atgattctaa ctgctatatc gcctctattt gcaactagta
    43021 ttttttttat caacttaaat acctcctact acaatggttt tatcttaaat aaaggttgat
    43081 tatattctac tagttcttca ttactaacta atacttcaat tatttcacct ttaatttcac
    43141 ttgttatctc attcataagc ttcattgctt ctaatataca taaagtgtcg ccttcttcaa
    43201 ctacatcgcc aactttaaca aagctatctg catctggact tggagaatca tagaatgttc
    43261 ccatcaaagg agctactact actgatagat tctcattaga tgtgacaggt gtatcgttta
    43321 caactggctt ttctaaagaa agatccacta ctgattcttg ttgtacactt aaaacaggtg
    43381 aacttgtaga ttgagctttt ttagatactt ctaatcttaa atcgctactt tcaagtttta
    43441 catattctaa gttagttgag tctatggcct ttaataattc cttaatttca ttgatgttca
    43501 ttttgttacc cccttgtatt attacctggt gctaatatta tatcacaaaa aacaaattta
    43561 taaaagaaaa agtgctgact agagaatcaa cacatcaata tatcttttaa ttttaaatta
    43621 acaaccagta acttatattt gttttaattt tccattatta atataaatta taagtataaa
    43681 atgaataaaa ttcaatattt tttgcaaaat acaagagaat acaaattaat acctcgggaa
    43741 tttttaagac aaataccaat attatgcaat ttagacttcg ctttctttca aattagtatc
    43801 attttcttta tttttacata ggatgtactt taatttagct atcaaaacat tgctatatag
    43861 gttaaaagtt attagaattg caggaattat aaagtaaact ctagcaccta taaaaccaaa
    43921 aactactgta ataagtatta atcttttatt taaagtaaaa aaaggtatat taaaaatact
    43981 tttgtcggat gtttctggag tataagaata tataactcca aaaatcaaag taagcataca
    44041 aagtggaagt agtgtataac ttgaaattat tcccaaaaat cctatacttt caaaatattt
    44101 gctagcacta tttgtataaa caaacatgta tccatttaaa tatgcgcata tacatgatat
    44161 aatgccaaaa gttcccccta aggattgagt gtaccaatta aaaaatttag attccatata
    44221 cctcacctcc taaaactgta tatataatga tatgcattta aatagtaaaa tatatcttta
    44281 tatgtttctg aataaaattt tgttaatttc attaatttat gtttaagaaa tttgaagtat
    44341 agtgtagtat atattaatgt agatgaagtt ttatagtact tatttcatag tgcttaatca
    44401 agttatctta ttatggattc aaaagttgtt tgtagggttt tatattttgt tgagctgaaa
    44461 aatttattat aaatgaaggg atgttttcgc tttggtagat acattagaat ttggtcttaa
    44521 aattttattt tttatattat caataatttg gatgggtaaa ataatgattt taagaactga
    44581 taagcaaata gtaatcaatc ctttgcttat tggtatatct gcagtccttg tgatgttgca
    44641 tacctcacag agtaacattg aattttttgg attagatgtc caatatataa gaatagtttt
    44701 gtatataata tattcactga taattttaat aggaatatgg gctactaaca gaagaaatgg
    44761 aattttttag ataatctatt tctataaaat ttaaaggttg ctagtaacaa atcactaata
    44821 ttagcgttct gttactaaca acctttattt tgattataca cttaaattga aaatagaatg
    44881 attgaataag ttgacagatt attttacatt gacagtaatt agaacttctt gagatgtatt
    44941 taaatcattt ttgtcgctta gtataacttt taccactgct tgacctgttt tattaccttt
    45001 aattatcata tcttcacatg aaagtacact cttatcactt acagatacaa taaaatttaa
    45061 atcttttgga aggttatcag taacttccca gtatttataa taactatcaa ttttcttcgt
    45121 aattattttt ctcaaatcaa tactttcacc ttttgctata tcaacattat gatatttaga
    45181 gtccagttta tactgattat cttttgtatc aataaaattt aaatcgcaat ttacatccac
    45241 accattatga gaaagagaat ttactacatc gtcatcaatc aaataattat tggcaagatt
    45301 tattgaatcc atatctttta gacttaaagc ttccttagga acctctttta tattattatt
    45361 ccaaaaaata aaattgtcta cattttcaag atatgatata ccttttaaat ttgaaatact
    45421 ctcaccagtt gcataaaatt ctccatctaa agcctttata ttgcttatag tcatctcatc
    45481 tggatttata tatcttttta caactgctct taaatttgca tcaggaaaaa tatctttaag
    45541 ttgattttcg ctcaaaacct tatcatttga atttattgga cttaaagctg ctgcagaaat
    45601 aataaatggt attaaattag ctaaattcat agtaccctcc cacataaatt ttttattata
    45661 ataatttaat ttctatgaga gggttctgtt tccttgttat atatacctac tttcgtacac
    45721 tttattaata taaattaaaa taaatttcta ctattaaact tataaacata ttactagtta
    45781 cgttttttag cttcacatct aaatattcgc ataccttgtg acataaattt gtcttcatac
    45841 tctgttgtaa cattattttc atactcacta tttcctaaat caagagaaat attctttaat
    45901 agccaattat ttgcagcaat ttcatttaaa ctaaactcga ataatttttc attatctgtt
    45961 ttaaagtgaa tttcgccatc atcttccaaa accctattgt acatttctaa aaatccagaa
    46021 tgagtgagtc ttcttttact ccatctattt ttaggccatg ggtcacagaa atttatataa
    46081 attctcgata attctttagc ttcaaagtaa tccaaaatat tgcttacatc tccccataaa
    46141 aatagtatat tattcaaatt agaagcatct gctttttcaa cagcctttaa aagaacttct
    46201 tccttaagtt ccatggcaat ataatttatg tcagggttct gcttagctaa agttgttata
    46261 aacttacctt ttccagtccc aaattcaaca tgtattggat tatcatttct aaactttaaa
    46321 ttccacttgc cttttaattt gtctatatcg tctcttaaaa catattttgt ataacttaaa
    46381 agcttttcat ctgcaccttt ttttcgtctt cttctcacaa taattctcct tacatataaa
    46441 aaattagtaa aatacatttt tattatacat tatctcacca aaaaataaaa agaactctct
    46501 taaaatagaa atttatttct ataaagagag ttctaaactt actaaaaact taatcttagt
    46561 ggcaccctga acaaccagag catcctcctc caccttgatc tttcatattt tcaggaataa
    46621 ctctaaatcc aaatcctgta tcttctatta ttatatctcc atactgacta tattcatcag
    46681 agctcattac aaattgtaat tctccaactt cataagttaa gtcatcttct tttttctcat
    46741 ctaaagctaa tccaaaagaa ggacctccac aaccaacacc tgcaaagtat actcttatat
    46801 tattaggttt atcttgattg tcttttaaga tactttttaa agtatcaata gctgtttctg
    46861 gtaaagtaat tttcatcaaa tcattccctt tcaaatattt tattaaaaca atatctatta
    46921 tatacccata caaggtacat aatcaattag tttttgcata ttttactaaa acttctacct
    46981 aatattacct aaggctttaa aatattttat ataatatttt attaccttaa ataaataccc
    47041 tatcataaac ttagtaaaca aataaaatta ttattctaaa aaatataata attttatttg
    47101 ttgttttaat attataacac atttgataat gtccaattca tatatgcaaa atttatatac
    47161 tttaaaatat ctaagaaatt attcaataaa aaaatactat tttttaaaaa ttatcattat
    47221 tgataaagct ctactattta tattttttag tgtattcatg tataataagt aaaagcacaa
    47281 cggtatatac tgttgtgctt ttttgtaatt aataaacaat attttggtct ataacaatga
    47341 aaaatggcta ctttccataa ttgatttcat ctgacataag caaaaagggt gtccagctgg
    47401 gtcaatcata actctccagt catcagtaaa ttgctcctct gcgattgtag ctccacaatg
    47461 aattgcatat tgaactgctt cttctaaatc attaacagcg aaatccatat gagccatttg
    47521 ctgttgagat tcaggttttt ctggttctat tatgccccaa gcctttgttt tttgcaatat
    47581 aaagaaaaaa ttatatattt taatatttta taattgttga aattgtgtaa taaattcaac
    47641 tggatacata gtgcgttttg aaatttcttc taaagctaaa ccttgtttta aaaacctctt
    47701 tatgacgctt tccatacttt ctgaaatttc tataatattt ttatcaaaat cataaaacct
    47761 aaaagttctt tgaccccatg ggtattcttt tatataatgc aaaaattcaa tatttcctat
    47821 tctatttatc ttactttccc attgtttgat gtcattaacc tcaaaataca actgaaagtt
    47881 atttgattgt tgatatattg gtaaatcata tccaattata gctttataat tactttgtaa
    47941 tccaaatcca ttttcaaatg atacatgttc tcctaaatct aatgtgattt tttgctccat
    48001 aacatcttca taaaactttt ttgatttatt tatgtctttg acaattagca atactcctcc
    48061 aaatttcatc ttatcactat cctttctttg ttataagtat tataacttaa aattttgcaa
    48121 tttcactgta aaaatcagac atattagctc ggtatttttg aggagttatt ccacagacaa
    48181 ttttaaaatc tttaataaaa tgtggtatat catagaatcc taattcatta catatagaca
    48241 tcaatgaagt tttattattg tttagaagtt ttatagattt attaatacgt accaaacgag
    48301 aaaatgactt catactcaac cctaggtact gattaaagag tctgtttaaa tgcctagaac
    48361 tataaaatac ttttttagaa atttcttgtg aagatatatt tccagagttt tgaataatca
    48421 actgaattgc caaaataaac tcatatgagt attcaatttt aatattagaa atcaataatt
    48481 cttccaactt taaaatcaat ttatctatag ataaagattc attaaatatt tttttaattt
    48541 ctaaatctaa tgaagtatta attatagaaa atggaacaac tttatcaata agttcacttt
    48601 gctgtatacc tataagcggg aaaaaaccag caggttgaaa ttctataatt agtataacat
    48661 cacatctatt agcaatattt cctactgttt ttggtttagt agttggacca aataatagac
    48721 tatgtaaccc tgtggcatca taaaaaaaca ccaaagttac acttccatga ggaataatcg
    48781 tgtaattatc agaaattatt gtttgatttg gaaacgatat agtaaaattc gatatccaat
    48841 ttcttaattg tatacaaggc aagagataaa caaaattgtc ttcacaaact ataagtgacc
    48901 tatttttatt tataaaatga atactttctt tttttaacat atattatatc ctgtcctatc
    48961 gtgaatattt attaaagtaa gaagaatcca tattatctct atatatcatt aactgctttt
    49021 aataatagag atagtatgaa tactcattag atatttaata aacaaataaa attaaaataa
    49081 tcaataaaaa atatagtact tttatctgtt attttaatat tatagcacac ttatagtatt
    49141 taacttatat ccactttaca tatgtctaat ttatataatt aaaaatgctt atctcaaata
    49201 caataattag tccttataca attaaaccat ctgaaactat aatagtacga ctactacttt
    49261 tggctgcttc tggtgagtga gttaccataa ttatagtttg tccattgtct ctgtttattt
    49321 tattcagtaa atctattatt tctatccctg ttttactatc taaatttcca gtaggctcat
    49381 cagcaaataa aatctctggt tttccaatta aagctcgagc tatagcaaca cgttgttgct
    49441 gaccaccaga taactctcgt ggtgtatgtt tacgtctatc agttaaacca actatatcta
    49501 agatttcatc taattgattt ttataatctt ttaaattttt accatctaat aacaaaggaa
    49561 gcattatatt ttcctccaca tttaaatttg gtattaagtt ataaaattga aatacgaatc
    49621 ctatatttct acgacgtatt atgctcattt tctcatcatt aaaatgtgat atatcagctc
    49681 cattcatata cacttttccg ctcgttggag tatctaaacc acctaaaata tataaaagag
    49741 tacttttgcc agaaccagat tgacccatga tagaaacaaa ttcacctttc attacttgta
    49801 aagatacatc ttttaacact cgagttgttg tatttccaat tttatattct ttgataatat
    49861 tttttgcttc tacagctaaa ctattatgtg taattttcat attaacctcc ataattattc
    49921 aaacttaatt tcttctatca gtttcatttt cttccctttt ataataggca ctatcgaacc
    49981 tattaaagtt actataattc ccaataaccc aacaataata aatgttttaa aatctaaatc
    50041 tggtttcatt gaaatttttg gtcctgcaac tataaagata gtttgaatct caagtataga
    50101 tatcacaata ccaataacag ctccaagtaa acctgaagta aacccttcaa tcaatgttac
    50161 ttttatattt tgtttatcac ttagtcctat agatttatac atagctatag aacgtctttt
    50221 ttgaatataa ttaatcagta gattattaat aatacctact gtagcaagaa gaaaaatgaa
    50281 ataggtcatc ttattcatag gagacaaaaa actacttata gtatttaaag aatcattatt
    50341 aaattcttca actgtacgac tccaattata tgtatctcca aataaatatc ttatctgaat
    50401 cataacagca tctggattga ctgctgtata tactaaaaaa ccataattag ttttatcaaa
    50461 atcattaact gcataatgtg atggtataac agcttctaca tcatttgcac gggatttaaa
    50521 actacctact attttataag aaaaatcata cttatcatta gatagagtta ttgtatctcc
    50581 tatagataag cctacttttt ttagagtatc ttcattcaaa ataacagaac gtttatcatt
    50641 aaaagcttct attacttgtt tttttatctc aaaatttgta taattgattc caaacataga
    50701 gttatatagt tttatattat ctgtaccctc tagtcttgat aatgttacac cattaccaga
    50761 aatctcatta ttcataacat aaagaggtaa tattttttta attccatcca tatgtctaac
    50821 atcttcaata aactcttcat tcatctttcc atctgcaaaa ccttgtaatt ctgcgtcacg
    50881 aaacacatct gttatataag ttttgacaaa gttaccaaca acacttattg caataactgc
    50941 agaaatactt ataaatagta aagtgatatt ctgtattata tttttgttat ttttcatatt
    51001 tctagcagct aatttaccct cattccctaa aatattttta taaacaaact caaaaactat
    51061 agacatgata tcagtaataa gaggaatcaa cacaatagta gctacaatta atcccagtaa
    51121 tgaaaatcca ccagctagat acaatgtatt ttcaggggat attcttggaa gtaaaataga
    51181 taaaataaac attatacttc ctataaaaag tatagaacga tttgaaacat tcttttcttc
    51241 tactgttcct agtacaatat tttttatcgg caaatggcta gcttttttaa caggtatata
    51301 tgcactaaat gaagaaacga ttattgctac tataacagat ataatcactc caatgggtga
    51361 tattactact ggaattgata ttccttgttc gagagaattt cctaacccat gaagcattaa
    51421 atttaaaaca actacaccaa taggaatagc aattaatcct cctatacttc cgtataatat
    51481 actttcaagc atcaatatac gagttacagt tttttcattg gcaccaatac ttctaaatgt
    51541 tccaataaaa ggaagacgtt ctaatgtaat aaccttataa ctgctgtata ttataaatat
    51601 actaagggtt aatgcaaaaa aacttattaa aaagaaaggc attgtctttt gtctagcatc
    51661 tgaaattatc ttagtttcat ttatagtatt tgataccata tatttttcag tagaaagttc
    51721 ttcggataat ttatttacta gattttctgt aatttcttct tctgactcta taagaactct
    51781 tgtatatcca ctgcctttat ttataatttt tgatagagtc tctttgggta gcagtgcatt
    51841 tacacctctt gtgttcctca aaaatactgt atcataagat gcaatatcac taacttgaaa
    51901 agtataggac tttccataaa tctgtaaagt aatagaatca ccttttttta ttttatattt
    51961 tgatgtaaag cgatttggaa gaataatttt atcaccagaa aaatcagtaa tactatcgcc
    52021 attttctaat cgtggcttgt tgattttatt tagttgactt aagtctgctg atataagact
    52081 aaattcttca tagtatcccc ctttattata aatagcagag ctttccaata caccaacctt
    52141 tgattttata gtatttaaat caggtatgtc ttctaaattg gttaatatat ctggattttt
    52201 actttggaca gatatagtcg ctgtaccata catacctttt gccatttttc tttgtgcact
    52261 ttcataagat aatccaattg agaatgaaac aaaaagtaaa actgttgata aaactatgga
    52321 caataacata actgctgttc taatttttcg ttctttaata tttgtgaaaa tatactttaa
    52381 tataattttc aaacttacac cttcttccct tatctaagat atttcaatct aatattttta
    52441 tactttaatc taaattaaat ctctaataga ctcctccata tttttatcat tatatttata
    52501 atatttttaa tcaaatcacc ccttaaataa aaaagattga taaactacta tataaatata
    52561 ataatctatc aaccttaata tattttttat ttgaccttaa ttaaccttaa ttaaatagga
    52621 aaggtgatat aaaatgagct acctaatcca ggtatacttt caacagatat attccctcca
    52681 tgaacttcaa taatttgctt cgttattgct agtcctaaac cagttccttc tattttttgt
    52741 tttgtattgc ttcctctata ataacgatta aacaaatttg atgcttcttc tcttgacatc
    52801 ccttttccat tatcagatat aatgatttca attctttcat ttaatgttat atgtacacaa
    52861 actttagtat ttttatatcc atatgtaaaa gcattgacaa taagattact aaaagctcgt
    52921 tttaatagtg tacaatcaaa atcaaagtta atattatcta tattactctt aaattcaact
    52981 atttttgatt catattctgg ataatttaaa atatcaatgg ataattcttt caaaaatctt
    53041 actaaatcat aattattttt atttaaaggg attattccat tttttaattg ataggttaat
    53101 tttaaatcgt ttataagtgt atttgtatat tctatgttct ttaacatgat tgaagcatat
    53161 ttatatactt tttcaggtga ataattagta ctactatctg taagaagctc tgcataccct
    53221 tttattggtg aaagtggagt ttttaaatca tgagttatat ttgaaatcca ctcttctctc
    53281 ataatatcag ttttattctg tatctcatca cttttatgaa tttcttcatt taaaatattt
    53341 aaactattat atatatcagc aaaaacacct ttaatattaa ctggaagata gcttcgtttt
    53401 gaaatatcgc taacagcaga tgtaatattt gatatgcact ttgttaccca gtaaccataa
    53461 gcaattccag aaaccaaaat tattaaaaat ataatcccta tgattgaaaa tacaattgac
    53521 ttaccaccta taaatttacc tccatcaaaa aacatattca ctttagatat atttaatgga
    53581 aaatatataa tataaatata gtcattttta ttatttttaa ttgtattaaa gtatacagtt
    53641 ccattacttg aattattttg gttagctaat gctaataatt cagaattaga ataataatct
    53701 ttatgctctt ttggttctaa aaacccataa actctatttc cactagaatc taaaatttgc
    53761 aaccacaatt gattttctcg aagtttttct aatccagact gttttacttg tggcttatta
    53821 tctataaata cgatttgttc attaaagttt tctgtaaatt ttttcggcca atcactacga
    53881 ataaattcac catttggttt ctgaatagta ataagtgata agaaaatacc tattactgtc
    53941 aggattaagc aaataaaaat catgaaaaaa ataatgtatg aatgaaatac tgttttataa
    54001 ctaggtttat gcatttaatc aactcttttc ttaaactttt attttagttt gattgttgct
    54061 tatataaaat agacaataat tattgatatg taaaaattta tttaaaaata caactatcta
    54121 cataaattat atttatataa ttatatgtta tcaagctgtc tgttgtattt aataaatcat
    54181 tttgataaca tataaaagta agataaattt ttccattaaa taccttataa ttatacacaa
    54241 aatctctata tacgttaaac agtatagaga aaattttcat agaaccatct ttatagggga
    54301 tactttataa aagaataaaa aaattataat atataaaaac ttcataggag tgtaacaaca
    54361 tcagtaaaaa tatgaaaaca ctatataaaa attcaaaaat attaagaata atgaaatact
    54421 tgaaattgct tatatcaaga aattttatat aatataaact ggtcaaatat gttataatat
    54481 taaattgtga caagttcatg gaggtgttta tatgaaaaaa caaaagtcgc caattttact
    54541 tgaaatgctt ggactatttt ttggttgtat atcaatgagt attggtataa atatgttttt
    54601 aaaaccacat acaattgctc ctggaggact tagtggtcta tcattagtac taaacaaggt
    54661 tacaggactt ccagtatcag ctatcatgtt aattataggt gtcccattag taatacttgc
    54721 ttttagaatt atgggtacaa aaaattcatt aaaaacttta tttggaacag ttgtattttc
    54781 agctatagtt caattaacag acccattatc aaaactaaga tttactaatg acttattatt
    54841 gtcttctatt gcaggaggaa tcctagttgg tataggttta ggaataatgt ttaaatcaga
    54901 tgcttctact ggaggaacag acttaattgc actcatactt agtaagaaat tcccaggaat
    54961 aaaagctaca aaattcatgt catgcctaga tggaatggtt gttatatctt ctggtatagt
    55021 aaatcgtagt ttagaaactg ctctttactc tggtatagca ctttgtgtat taataaaaat
    55081 agcagatatg ataatggaag gatttgacca ttcaaaagca ttttttatta tctctgatga
    55141 acctgaaagt ttaagacaag ctataactga agaacttgat agaggtctta ctattcttga
    55201 tggtaaaggt ggatacacta gagaaaataa agaagttcta ttagtcgtag tttctaaaaa
    55261 acaagaactt tatctaaaga gattagttaa aagaactgac cctactgcat ttgttattgt
    55321 tacagatgta catgaggttc ttggagaagg atttaaaaat ttagagaact aataaaaatt
    55381 aatattttaa gtatatatac tattttaatt aaaatacgat ttattcaaaa agagtgtctc
    55441 ataaactttt tagttcatga gacactcttt tttgtataaa atcaaatgca ttttcagtat
    55501 tttaacaata aaaacaggat tatctatatt ttgaggcaac cactttattg aaattttatt
    55561 tagaaaacaa tcattactaa ataaccagca tagatattaa atgattatat ctattgtttt
    55621 ttcaattcat attgataaca aggaacattt tttacagtat ttccagtatg aatttttttg
    55681 atatactttc ctgtacattt aaatcctaat aaatctaatg attttttaga attttggttt
    55741 tcttcatagg ttattgcaat tattttattt aagtttagtt ctctaaatcc atactgtatt
    55801 actctttttg ttatctcagt agcaaaatca tgtccccaat agctcggatt tatacatata
    55861 cctatttctg cttctaatgc tttaggattt atattattaa accccatatt cccaataact
    55921 ttattgtctc ttttatatac tatagcaaag tggccaattt tccctttttt atatggattt
    55981 agaaaaaatg attttataaa ctttcttgta tcttctaaag atttatgctc actaaatggt
    56041 aaatgtttta ctactatttt atttttataa cagtcaaaaa aatcttcaac atcatctaat
    56101 gatgcaggtc ttagtataca acttctagtt tctaaggttt tcatttgagg atattcagaa
    56161 tcagacattt ctaatccccc tttatacatt aatctaaata tactgttcat atttaaaata
    56221 cgatattaat tatataaatg ttctcaaatt aaccttattt ttatttttaa taaaaactaa
    56281 ttactatatg aatctttcaa gtttacgaat ttatctctat ttttatatta aattacttgt
    56341 gaaatatgta ttctaaatta tatattatta gatatcatgc ttaaaaattc tttggttgct
    56401 aaggataaag atatatcttt taaatggcag taacctatat ttctcttagg tattggctct
    56461 tttatatcta atttaaacaa tttctcattt tccaaataat ctatgctaaa ttcttctata
    56521 acacaagaca ctccaagatt tatatacgca aattctaaaa gtaattcatg agaacccaat
    56581 tctatatccg ggtttagttt tatacctttt gataaaaaat attcatttac ataaagtctt
    56641 gaatttgctt tattttctag cattattaaa ggcaatgtgt ttaattcttc caatgatatt
    56701 ttcctacctt tgtattctat gtaatcattt gcacatacaa atatatcatg tatttcaata
    56761 cactcaacaa tatttagtgt atcatcttct attggcatgt ttataattgc tatatctata
    56821 ttcccatctt tgagtaaaga tataagttct ctactcgttc tatttattgt agatacatgt
    56881 atatgtggat acttcttatg aaaaatttct aaatatttta acaagaaaaa acgtgcagct
    56941 gtatctccta ctcctatttt cagagaaccg tattctaatt ttttaaattt atttattcta
    57001 tcttctcctg atgatattaa ctttaatgca gtagtgacat gttcacttag aattttcccc
    57061 tctggagtta attttactcc cttattgttt ctataaaata ataaagtatc taattgttct
    57121 tctaattgct ttattgactg gcttacagct ggctgtgaca taaacaattc cttagcagca
    57181 agagaaaaac tcttattatt tacaactgca ttaaaaacct tataaagttc aagttttata
    57241 ttcatatata attactcctt atgtcaaata ttagtattat ttattttact tataattata
    57301 tttgaattat aatatacatg gtactaatat gacaatatta attctttagg aggattttag
    57361 ataatggata gattagttgg aactgtttca agaggggtta gagcacctat tataagacaa
    57421 ggagatgact tagttaaaat agttgttgat tctgttttaa atgcgtcaaa aagtgaaaac
    57481 tttgaagtta gggacaaaga tgtaattgcc gttacagaag ctgttgtagc tagagcacag
    57541 ggaaattatg ctcacgttga caatattgca aaagatgtaa aagataaatt tggagatgat
    57601 actgttggag ttatattccc aattttaagt agaaatcgtt ttgctatatg tttaaaagga
    57661 attgcaaaag gatgtagaaa agtcgtatta atgttaagct acccttctga tgaagttgga
    57721 aatcacttaa taagtattga tgaacttgat gataagggaa taaatccttg gtcagatgtt
    57781 ttaacagaag aaaaatacag agaactattt ggatataaca agcatacatt tactggtgta
    57841 gattatgttg attattataa gaatttaatc aaggattgtg gtgcagaggt agaaatagta
    57901 tttgcaaaca atccaaaaac tattcttaac tatacaacaa gtattttaac ttgtgatata
    57961 catacaagac aaagaacaaa gagaatttta agacaaaatg gagctaaaaa agtatattca
    58021 cttgatgata taatgacagc aagtgtagat ggtagtggtt acaatgacca gtatggactt
    58081 ttaggttcaa acaaagctac agaagaaaca gtaaaattat tccctataaa ttgtgatgaa
    58141 gttgtaaaca aaatccaagg taatataaaa gaaataacag ggaaagatgt agaagtaatg
    58201 gtttatggag atggagcttt taaagaccct gtaggtaaaa tatgggaatt agctgaccca
    58261 gtagtatctc ctgcttatac aaaagggcta gagggaactc caaacgaagt aaaattaaaa
    58321 tacttagcag ataatgattt tgctgactta tctggtgatg agttaaaaga agcaatttca
    58381 aaatacatag ttgaaaaaga taataaatct gatgatttaa caggaaacat ggtttctcaa
    58441 ggtactactc caagaagatt aacagacctt ataggttctt tagcagattt aacatctgga
    58501 agtggagata agggtactcc tattatttac atacaaggat atttcgataa ctatacaaaa
    58561 tagtatgttt taataagatt taaatttttt ggattatata tattaacatg aaaatttatt
    58621 aaaacctctt aatagatatg acttttcaag agttatatct attaagaggt ttatttaatg
    58681 ttcacagata agatatattt aaataattta aacaagattg tcagaaagtt atataatctt
    58741 aaaaatttat ataactctaa ttcttattta tttctgaaat aatatcactc ttcaaaattt
    58801 gtcttactgg aataattaca gcaactatac taatagtgat atttaaaata atcataccta
    58861 tgtatagagg tgtatttatt ttaagaattg gatttatata taagtataca cgttttacca
    58921 taaaataaat cataaattgt ccaataacac ttccaattac cattataata cttgcataga
    58981 atccatataa aaaaccttct cttatcatca taagtaaaaa cttattatcc gtaataccca
    59041 tagctcttaa aattccaaac tcatgtcttc ttgaaaaaat taagtagctt actgtattga
    59101 ttatatggaa caaacttatt gcaagaagta caaatacaat accatatata aataagagtt
    59161 tttgatttaa gtaggtatta tttttttcaa tagcaatggt ataatctgtt acaatacatc
    59221 tttttacatc acttacaaca ctttttatgt tctcagacaa ttcctcactg ttactatctt
    59281 ctgtcttttt tatacttact gaattataat tttcgatatc aaagttattt ttcatttgcc
    59341 cattcgtcat aactatatcc ataccataat caccaatgaa atatatgtta tttgccatca
    59401 cacgttttac agtagcagca actacaaatt cttttgttgt ataatcattc ttattttctt
    59461 ctttaaattt aacaatttca tctgttggtt tcattgtctt tggcacttta acttttattg
    59521 tatctcctgg atttatatca actgcatcat acataccagt tccatccata gggagtctaa
    59581 ctataacctt attattttta accatatcat gggtattaat ctccccatct ataatataag
    59641 aatttaatcc ttctaacata ccattgttat aaccataaat atttgttttt aaaagatagc
    59701 caccttcatt ttgctttgta caaataccat caaaaaaagt ctttatcctg taatcttttt
    59761 caagaggttt aaagaaattt ttccaaaata gttgttcttc tttaatttgc atagctccaa
    59821 ataaatatct tactggatag acttttgata ttccctcaac attgtttaat cttgaaattt
    59881 tatcttttgg tatacctaca tcaaaatctg ttgtttgcat agttatttgg tagtcagaat
    59941 ttaaaccatc atcagctttc attgtaagtt cattattatt tttagtaagc tgtatagagt
    60001 aactagaaca tagaaatata attccaccaa gtgataatga aaacaaaatt ccaataaaca
    60061 ttcccttttt ttgagtcatg tatttatgag ataatacttt tgtcatgtta gagtgtttta
    60121 cactttctaa tattctcttt ttcttattag taatcttaaa gtttttcata gattctatac
    60181 tagagtattt attcaactga tgtacaacac gtacagcaat taaaattaac aacaataaga
    60241 taaataaaaa gcctaaataa atagattttt cacttataaa aaatgctcct ggaacaatat
    60301 cactactcaa aaatatatgt ggaaattgtt catatattgt agacgccact tcaataccta
    60361 agaagcaacc tataggaaat ccaacaataa aaagtaaaaa taactctaaa aatagtagaa
    60421 ataaaagttg tttattatta gcgcctaata cttctaaaac cccatattgt gatattctct
    60481 gaagtattga aacatggaaa atactatata ctgctgctgc accaaacact ccaacaagta
    60541 atataattgc atttgtattt agttctaaat tttttaagaa ctcaaatata ttatattttt
    60601 ctatattttt tggtggtttt actccaaatt caactgaaag aggccagttt atataagttt
    60661 tttcttttga tatgttgaat tttttcataa atttataatt ttgcttttta atatttttat
    60721 tttcatcata ttttacataa actatataag aatccttttc tttaggctga aaataatcgt
    60781 ttacaaatcc ctccagagca gtttctgggt ttgaaagact tgataaaata ccacataact
    60841 tatagatttt accatctaaa ttgatttctg ttccaatttc tttgtttaca tttaaattgt
    60901 ttagagaata tgtgtcaagt actatctctc catctttact aggaagagtt ccttttaata
    60961 attttctacc tgtcatgtct aaatatgaag aatcagcatt taaaaattct ataataaaag
    61021 gttcatctat ctgattttta ggttttgtaa tgccaagtct atctacagtg tattcctttg
    61081 attttttatt ttctttaatt ttattaagtt gttccttatc tactttatag aaataatgat
    61141 aatctccact atcttccctt atcttttcaa ttctactttt gtctgcacta tgaactaaag
    61201 aaccaattcc cgtcaataat gctactgata gtactatgct taaaactata gatatagatt
    61261 gttttttata atgctttata tattgaattg aaaattttac tagagacatc atagtacttc
    61321 accaccagta ttcaccacaa ctccatcttc aatatggata attgagtcgc atacttgtgc
    61381 tatattttca ttgtgtgtta ccataagtat cgtttgatgg aattttgcac tactttcttt
    61441 taataaacat accacttcca ttgtagtttt actatcgaga tttcctgttg gttcatctgc
    61501 taatataagc gcaggtttat ttgccaaagc tctagcaatt gctaccctct gttgttgacc
    61561 accagatagt tcgcttggaa attttaaacg cttatcccaa attcccaatg tcttcatcag
    61621 catttcaata tattcatggt ctatatggtt tcctttatct aatgtaacag gaagagctat
    61681 attatcataa gtgtttaata cgggcattaa actgtagttt tgaaatataa atcctatatt
    61741 tcttcttcta aatactgtca aatctttttt tcctatttta gatatgttct ttcctttaat
    61801 tattacatca cctgaagttg gattatctaa tccacctata atatttaaca atgtgctttt
    61861 tccagaacca gaagttccaa ctatagcagt aaatgtatct cgttcaattt ctatacttac
    61921 tccatttaag gcttttactt tagtctcacc ttttccatag tgttttacta aattattcgt
    61981 cactaatata ctcatttaat ttctcctttc aatacagata tatgttctat aaaatattgt
    62041 aagtgacgat aattacactc atcctacaaa attagtttaa actcttacaa ttctgtaaga
    62101 agatactaaa acaacatcca cttcctagtt tagattctac tataatgttt ccattttctt
    62161 tttctaaaat taatcttgta agatatagac caatccctgt gccattttgg ttttccacat
    62221 ttttacttct gtaaaatctt ttaaaaatat catttagttc tctactatca atacctattc
    62281 cctcatcttt aatagtaatc ttcgtatata gttccatttt tattacagaa atagtaatcg
    62341 tgctatctga tggtgagtat tttattgcat tttctaaaac atttacaatt gcttctatgg
    62401 tccacttttt attatgaact aactttatgt ctgaaaatgg ttcgagattt atccttatat
    62461 ttttagcttc tgctttatta aagatagtac tgatactttg aataagagta tctttaatta
    62521 gtagttcttc tacttcaaat tctataactc catcctctaa tttagtcatt ttaaataagg
    62581 attgtaacag ccagtcaatc ttaaaagttt catttttcat cttttgtaaa aactccttct
    62641 tctgtaatgt ggaaatattt atattctcca gaagttcaca ataaatggtt atatttgaaa
    62701 gtggagtttt taattgatgt gacatatttg atattaaacc tttaatcgct tctttttctg
    62761 actttgactg ttctacctct atttctatca tatccttaat tttttttact tggtgagaaa
    62821 tttttgacgc tcttgtatcc tttgtatctg gataagtaac ttctttacct tccaatattt
    62881 gatttaacat caaatttaat tcatcatatc tttttcgatt tattttagta gatactataa
    62941 atataacaac tgtacatatt ataaaggcta atataaacat attattctac ctcattccat
    63001 aaataaccca ttccataagc tgtttttata tattttggat tagatgggtc atcctctact
    63061 tttactctaa gtcttcttat atttacagat acaatattat catcaacata attttcttcg
    63121 ctgtcccaaa tatggtgtaa aatttgttcc tttaataata tttgaccttt attttcaatt
    63181 agataactta gaagcttata ttctacagag ctaagttcca aacattcttt atttttatat
    63241 acttttagta acttttggtc caattttatt ccattggaat atattacttt attattcgat
    63301 ttttttctta ttgctacttt gactcttgcc tttaatacag ataaagaaaa tggttttgta
    63361 atataatcat caagccctat ttctaatcct tttacttcat caatttctga gtctcttgca
    63421 gtaagcatta taattggtat atctgacttt tcccttatta tttgacatac atcaaatcca
    63481 tatccatctg gaagattgca gtctaagatt ataaaatcta tatttttact attaaatata
    63541 tttaatcctt cttgttttgt ataagcctta aatacattaa atccatcatt ctcaaatgca
    63601 taaaaaagac cttcatttaa gtttatatca tcttcaataa ttaataagtt caattttact
    63661 ctcctttaga ttttttagat aaataattat tttcttgcat tatcatacta aaaatatttt
    63721 aattttattt atttcactaa aaagattgta tttaaatttg ttttcaaaat aaaaacatca
    63781 taatcaagca tatagttata tatttcccaa tttaattacg cttaaattga aaatataact
    63841 ttattcttat ataattaaat tatacaacaa taaagttata ttttttaaat atatttttac
    63901 tagggggaat agaattgaaa agggttgatg agagaaatac tatgtttgca agagcaaatt
    63961 acaaaaaaga tagtactgct tataatgatt attataagaa aaatcctgac aaaaaagaaa
    64021 ttgatgatag tataagaaat agaccaaatt tatgtagtga aggaacaatg acttacaatg
    64081 agttaaactc tccaatggca agtagtgcat tcgatttttt atctgatatc aaatctttat
    64141 gcgaaggaaa agtttctgat actaaagtag atgtagatgc aaagtcaatg acaaaaaaaa
    64201 ttaagggtct tgcaaagcag tatggagctt ctgtagttgg tataactaaa ttaaaagact
    64261 atcatattta tacaaatagg ggtagacatg aagaaaatta cggtgaagaa attaatctaa
    64321 ctcataagta tgcaattgta tttggatgtg agatggataa agaaatgata aatagagctc
    64381 caatgatatg tgaagttata gaaacatcaa agtgctatgt ggatgcatct atcgtcggca
    64441 tgattttaag ttactatata agaaatttag gatatgatgc tagaaatcat atggatgcta
    64501 attatttggt aatgcctgtt tttatagctc gtgatgctgg tttaggagat ataggaagaa
    64561 atgctatact tacgaataaa gattatggct ctagacttag attaggtgtt gttactacag
    64621 atattcctct tttagaagat gaatatgttg attttggact agaagatttt tgtaaggtct
    64681 gtaaaaaatg ctcttttaac tgcccttctc attcgctttc taatgatatt aaaattggtg
    64741 atgatggaaa atataattgg gtaattgaac atgagacatg ctatattaaa tggagatatt
    64801 taggtacaga ttgtggtatg tgtatatctg cttgcccatt tagccaaaat ctcgaaacta
    64861 taaagaatac tacttcattt aagggtaata atgagcttat tcaaaaagct ttagatgagt
    64921 atacttcaaa atttggtaaa agaatattta tacctggaaa tccatcttgg ttgaaataat
    64981 tttatttaat aaagaatctg tactagaatt tatcatagga caggttcttt attaaatata
    65041 taaattttta aatacttttt ctcgtttata acaattcagc aatgttttta gtctataatt
    65101 gacattataa aatattaaac taaaaggagt aactgtatgg aaaaaatatt agtagtagaa
    65161 gatgattcta tattaaataa aactctatca tataacctaa ttgaagatgg atatataatt
    65221 acttcaaaat ttacagctaa atctgcactg aagtctattt ttgaatgtga atttgattta
    65281 ataattttgg atattaattt accagataaa agtgggtttg aattatgtaa tgaaataaag
    65341 ggaaattaca atataccaat aattttttta actgctaatg atatggaatg tgatatgatt
    65401 aaaggctatg aacttggagc tttggattat ataacaaaac catttaatat caatatattt
    65461 aaacagaaag ttaaagcttt tttaaatcat cttacaataa aaacaaaaca agattactat
    65521 agggatggat atcttgaaat taacttttct gagttatctg ccaatataaa tggaaatcaa
    65581 attatattta caccattaga atatcgtact ttaaaactgc ttactgaaaa tcctaaaagt
    65641 attttaacaa ggaaagtact tttagaaaaa ctctgggata tagatgctaa ctttgtagat
    65701 gaacatactt taacatctgt tataagtagg attagaagca aaattgaaaa agacaatctt
    65761 aagtatatca aaacggtata tggtatggga tatatgtggt taggtgaaag aaatgaattt
    65821 taaaaaattt tcttttagtt ctatttattt aataattgct gttggtataa tttttacagc
    65881 tattttattt agtatctcaa tttatctaat aactgctgat ttaaaactaa ttgctctatt
    65941 attaatatat acaatagtgc tttgtggatt tatagcatta ataatatttt tacttcataa
    66001 aaaaatagtt atattttgtt ctaaaatatg tcacacttta gatagtatga tgaatagtga
    66061 agacaatatt gaaattgaat tagaagaaga aactatttta tcacgtatca atcacagatt
    66121 aattagaatg tatgaagtta ttcaagaaaa tcgcaatagt attgctatag agaaagctga
    66181 tttacaagaa ctagtttctg atatatctca tcaaattaaa actcctattg caaatttaaa
    66241 aatgattaat actactctac taaatcaaac atttaaaaca gaaattcaaa aggatttttt
    66301 aatggatatg gaaaaccaat tagataaatt agatttttta atgcaatcta tggtaaaaac
    66361 ctccagacta gagacaggta taatcacact atctaaaaag aaaaattcaa tttatgaaac
    66421 aattgctaca gcacttagtg gaattttgtt tgatgctgaa aaaaagaata ttgaagttac
    66481 agttgattgt gacccaaatt tatatataat tcatgacaag aaatggactt ctgaagctat
    66541 ttttaatata ttagataatg ctgtaaaata cacaaattct aatggtaaaa tacgtgtagc
    66601 tacagaatgt tgggttatgt atacaaaaat agatattatt gataatggaa aaggaatatc
    66661 agaaagtcat caagcagaaa tctttaaaag attttatcgt gaagaagatg tccatgacat
    66721 agaaggtatt ggaataggtc tctatctttc aagaaaaatt atatctcttc aaggtggcta
    66781 tattaaagtt acatcagata ttggaaaagg gtcgactttt tctattttct tacctaataa
    66841 ataaattttt aaatatttca tttgtaatga ttctgcaaca ttttggtttt atactgtatt
    66901 tacaagatgt taaggaggta ttattatgag tattttacaa gtaattaatt taaagaaata
    66961 ttatggacaa agttcaaaca ttacaaaagc tcttgatggt gtaactctat ctattgaaaa
    67021 aggcgaattt atttctattg taggtacatc tggtagtggc aaatccacat tattaaatat
    67081 gatgggagga ttagataatc ctacatctgg aaaggttatt ataaagggta aggaattatc
    67141 tactatgact gatgaacagc ttacaatctt tagacgcaga aatataggtt ttatatttca
    67201 aaattataat cttgttcctt tacttaatgt atatgaaaat attatcttac caataggatt
    67261 ggatggagag accatagata aggattttat aaatgaaatt ataaatatgc tttctcttga
    67321 agaaaaatta tatgatatgc caagtaatct ttctggtggt caacagcaac gtgttgctat
    67381 agctagagca cttgtaacaa aaccagctat tatactagct gatgaaccaa caggtaacct
    67441 tgatagtaga actagttcag atgtactgag tttacttaaa gtgacaagta aaaaattcaa
    67501 ccaaacaatt gtaatgatta cacacaataa tgaaattgca caactaactg atagaattat
    67561 tcgtattgaa gatggaaaaa ttgttaacta ggaggtgtag tatatgttta gaaataataa
    67621 tcgcacattt ataagaaaaa tagctttaaa tgatttaaaa attaataaac ttaaaacata
    67681 tctttcaggt atcattataa tgatttctac atgtttatta ttaacagtga ccttagtttc
    67741 atataatgcc tctgtagatt tagttaatgc atctccatat catgctatat ataaatctgt
    67801 agatgaaaaa gcaaaaaata tactatataa aaataaaaac tttagtggcg taggaactta
    67861 caaattaatt ggaagtaata aaaagacaga ttgtattatg tcgattgttt atgcagatga
    67921 tacaacaatt aaacttatga attttcaacc attgaaaggc aaactaccta caaataaaaa
    67981 tgaaattgct atttcagaaa aatatttaca ggaatttggt ttagataaag atattggaga
    68041 tagcattaaa ttaaattatt atgatgatat tacgaataaa gaagttcaat gtgattttat
    68101 tattgttgga tttctagaaa attattatca agataatgcc aagcaatact atacagtcgt
    68161 ttcaaatgat tattttaaga atatagcttc tacaccttta caaaataaaa gtaatacttt
    68221 tgacgagtca agacctgata ctgtagatgt attagttaaa ctatcagaag aaaatacaga
    68281 gaaagattca gcttcaatta agacacagtt aaagaaaatt ggtttatctt taggtattaa
    68341 gaaatataat atatacttaa atgataacta tattgaatca aatttaattg atggtgagca
    68401 gattattaca gttttagtag ttggaatagt tgttcttttt tctagtgttt ttgttatata
    68461 tagtattttt tatatttcag ttgttaattt agttcagatg tatgctaagt tgaaatcgtt
    68521 aggtatgact tctttccagt taaaaaagat tatttttcta caaggaaata ttttgtcaat
    68581 tatttttatt ccattagggg ttatagcttc ttgtataatt gcttacataa ttcaaccatt
    68641 agcatggcaa atgatagctg atttatttat aattttaatt ttatcactgg taatgttttt
    68701 aactgttaga atttctttat ttaaaccagc tagaattatt tctaaaatat ctgctattga
    68761 agccatgcaa tatacagaaa ctaaatttag aaaaaaagta aaaaatttta gttatataaa
    68821 tataaaaaat ctagcattaa agaatatcga gtcaaataga aagaaaaatt tgattgcatt
    68881 aatatcgcta agtattagtg gagttttatt tatttcaatc gctaatcttg ctaattcaat
    68941 agatttcaaa aaacaactat ctcaacaatt tgtctataac gaagattata taattagcat
    69001 taagagtgat aacttatatg agcatattac agaaatccaa caaaataatc cactaacaga
    69061 ttctcttaag aatgaaatta aatctattca tggagtaaaa aaattaattg aatccaaatt
    69121 aattaaaagt aatattatag aaccttacat caaagataga gacgaaaaac aatttattac
    69181 tctaattaaa ggtattacgc ctgaactttc aaaatcactt gaagaatata ttgtcagtgg
    69241 aaaaatagac tatacacatt tagattcaga tagtttaatt ataaataaat atagaacaaa
    69301 atattatgga ttaaatttaa aagttggaga tacagttaca tttaatatct ttagtggaaa
    69361 tgatattatt aaaaagaaaa tgaaaattat aggaatcatt gacaattcca atacagaagg
    69421 tatgtttttt gcttcagaca aaactttaga atctctaaca ccatataata caaatttaga
    69481 tttttctatt ttaatagata aaaagtattc taaagaagtt gaagaaaagt tgttaagtat
    69541 aattcagaaa aattcaaatt taatgcttta ttcatatgaa gatgattata gaatgattac
    69601 tagagcattt caatatataa ttatagcttc atacattttt gttggtttaa tttcttgctt
    69661 tggtatacta aatatgacca ataccctaat aaacagcgtt ttaattagaa aaaaagagtt
    69721 tgccttactt caagccgttg gaatgacaag aaagcaactg cgaaatatgc tttatagaga
    69781 aggtctaaac ataagtatca aagctatatg tacttcaagt atactaggtt attttggttc
    69841 aaatctactt tgcactttta taaaagatgt aattagatta gactttatta attttaaatt
    69901 ttctatattt actattttaa ttttttcatt cgtattaatt ggtatacaag ttttagtgac
    69961 tgaattactt gtaagaaata tagaaaaaaa atcacttaca gaacgcttac gttctcagta
    70021 agaatgtata taaaaaatag ccatttttta tacaagtttt aacataattt tttaaaagct
    70081 aaaaggatag aaaattttga ctttacataa ttttctatcc ctatacttgt ttttatttta
    70141 tacagataca cttccttata tctccgagca acatcaccag agacataaac aagcatattc
    70201 cttattttct aaatatgtca attgcatgac ttgtaacccc tactaaatct tcacgttcac
    70261 aagtagcaag gtgatatttt aagtataatg caaatgcatc atcatccata gagtctactg
    70321 cttcacgcat atacaaagcc aaaccatctg atgcgacata atgcagtcta gttacattaa
    70381 atgcagacat caaatcatca atgttttctt tacgtacaag ctcaaacaaa tcctttggtt
    70441 cagacttagc agcaaatgtc tgtggatcaa tcaaaccttt ttcaatatat tcagatacat
    70501 taatatttcc tctatggaac ccctcgtcaa tgagacagcc atcagatata acatatgcag
    70561 caaaaaccac accaccaggt tttgttacac gaattgcctc atgtaaagct tgttgtttat
    70621 cttctttgtt ataaagatga tatagtggtc ctaacagtaa cgtaatatca tatttattat
    70681 ctgggaaaac agataaatcc atagcattac cctgagtaat gcttatattt tcatttgatt
    70741 gagtattttt atggaaaacc tctatattat gttcgactaa ttccactgcg tcaacatcat
    70801 atccctgacg tgccagtgtg tgagaataac gaccagttgc agcgccaatt tcaagcacac
    70861 aatcacctga ttttatgtac ttatcaatgt aatgcatagt agtgagaaat tccaccattc
    70921 catgttttaa tgcaagtcga ctatcttcat cataagtatt ataaaagtca attaagtatt
    70981 gttttgtttc cataatttca cctcttctat attagttaat aaacatcaaa tagacaaatt
    71041 ccaatctggt ttttcttgtc ttataaaagc tgttaatata tttttcaaca ttttttcagc
    71101 actatatcta tctaatgtta gtttttttgt tgctgttaag acagctatgc catgagtata
    71161 aagaaataaa tctaaaaata ttacttttgc acattccaac tctattccaa tgctttccga
    71221 aaaaagtctt gccttttttt cattgtcaat ttctttataa aaatcattcg cctctttcat
    71281 catcaaatct aaatcattta taaataacaa cttaaataaa tacgtttctt cttgagcaaa
    71341 ttcaatgtat gaaagcggaa gcagtaaata agggcttaca cttttagaat tacgataatt
    71401 aacaacatac tgattataat attcatatgc aaaagcaaga aattcatttt ttaattcatt
    71461 catgttttcg taacaagtaa aaattggtcg agtggaacac tgtaatttac ttgcaatact
    71521 cctcgcattt acagcttcaa atcccacttc tcttgtaata tccaagactg tattcaaaat
    71581 catttctttt gttatcttta ctttaggtgg catatatgtt cacctctcca ttaaattttt
    71641 ataaaacaca tatgttatat aactatagta acatatcaag aaattatgtc aagaaaaagc
    71701 tttgcatttt tttgcaattc ctaaaactta taaataaaac atgagttgct gttatctatt
    71761 aatagataac agcaactcag attttccttt aaatactctt ctttttgtct aatatttcat
    71821 atacacaatc ttctattgaa ttaatatctt tttcttttct ttttttagaa tcaattaaaa
    71881 tgaaatacag tataaaacct acagcaagtc cccatgttcc gcctttagca gcaattacac
    71941 ctcccataag tccaatcaat cccttctcaa cacctgtatt acatagctcc attgatagct
    72001 ttgtacataa atatccttga actaaaagtg ttactgacaa tgctaatggt ataagaggtg
    72061 tagcaatctg cgcaattgga taaaagcaga taaaaattgc tgaaacccat actaatgtac
    72121 ccataccact aaaaacagac tgcatggctt cctttccaat cttgtacctt tgagcaacac
    72181 ttcctgtaag tgtcgcagct aatggaccac acattggtat atatggagaa aagattgcca
    72241 taaaaatatt tcttatccca cttataacat tagacctatt tgaattgaaa tctatatatt
    72301 catcttttct attttcactt tcagaaagta ctaaattttc acctgttata aaatcaccaa
    72361 atgcaattat gtatatcata aaagccattg gtatcccttg tataaataac attacacttg
    72421 gaaaacctat tccaaaagga gatactgtat ttatcagatt tttaaaatct actatgtaaa
    72481 ctaaattgtc aaaactaaat ctaggaattt ctatttctct actaaatata cctactatta
    72541 aactcactaa aatagctgat actacaccat attctcctaa atgaaataaa aatttattct
    72601 taacttttag agtatcaaat ctttcagaaa acattacaaa acaagcaacc aaaattccta
    72661 ctgttataga tataggataa gaatcaaatc tccctttagg attaaactct gatattatag
    72721 tcgatatacc gacacccatt agtattccac ctttaataga atttggtatc ttactaacta
    72781 actttccagc tacaccagtt atacccatta aaataaatat tattcctaaa tctatttgta
    72841 aagctgttaa tgcttgtatt ctttcaatgc ccatgctgaa ctttaacaaa tatgcagttg
    72901 taagagttat agaagctgta atccagccag gtacaactgg gtctcccata aaaacatgca
    72961 aacagtataa tattgagtta atgatagcca tactccatgc cacttcatag ggtaatccca
    73021 atatttgtgt actaaggcca attgtgccta aacttgtcat acttgatatt ataccttgaa
    73081 tagcttctgg tttctcaaat ctgtaatgta taaatggaat tcttatctta aacattccaa
    73141 attttatatg aggttgttcc cttctagcga gtctttccat aacttcactc cacctttcaa
    73201 taactcttgt aagtttacaa ctttataatt gtttataaat tatcaatgtc attaaaatta
    73261 gtatataaca tgcaaattat ataaacaata aattaaatct ataaaattga tagattttgt
    73321 gcatatatag aaagaccact tcatttttag ttacttaaat cacttaatat tagttattta
    73381 aatcacttga taaaggtttt ttataaaatt caatagttct attccatgat tcaaactgtc
    73441 cttttgaatt tccttctaca gttccacctg aagaaaaaat ccctccactc atactcatac
    73501 ataaagtctc ctttagaggc ataatatatg gaattggtaa cggatgaccc atattattgt
    73561 atgttaataa ttctacgtta tatggataat tattttcttc aaagatttct aatgcctcta
    73621 aacaagcttc ataactattc catatgttat cttcttttcc agcaatcata agtatatctg
    73681 ctttggaatt ttctattttt attctagcct cttttctatt ctctgctctt ttaagacttt
    73741 tcttataagt actattaaat ccaaatggaa tatcttttga tatatttttt ttctgttctt
    73801 caaagaatat atcattgtct actttgatat atggaaatgg ctctccttta tatgaccatg
    73861 atgaagtatc atttccactc atcaatccat taagagcttg gaaacaataa atgtgaggtt
    73921 gtgatactac aaccttttta atttgttcat atcttgaagc aagtaaaaag gccaattctc
    73981 caccctttga tgtcccatgt acaaatattt ctttagtact ggtaatttca ttttcactaa
    74041 tccactcaaa tactttttca aaatattcta atggaacctc ttctaattta tctggtaacc
    74101 cttctaaagc aaagtatgca actgttagta cattgaatcc tctagatgca agtggagctg
    74161 ctaaaaggtc tagagcgtct aaatttccat cagaaccacc tagcattaaa attgttttat
    74221 tattagaatt ttgtttataa aataattttc ctataaactc atcagatatg tattttgtgg
    74281 taatatcttc tgatttaaat attctcttta attcaacttc ttttctttca atagaagttt
    74341 caaatatcat attcataatt attggtttat caatagatat attttctgca aaatttttac
    74401 cttcagtttt agatttttcc agcgaataaa ttaaccccat acaattatca gccttataag
    74461 taccttctat aggttcaact aaatctaaat ctacttctcc tttttcattt gaacaaaaca
    74521 ctccataaga tgaaaattct tctcccttgc accatggaaa ttccattttc acacttattt
    74581 taagtttagt atttggaatt aagtccgaaa ccataatctt aagtctttca tccacaaatg
    74641 attctacatt gtatacattt actttcataa tctatttctc cttataataa ttatttatgt
    74701 tttatataaa acagctttat ctaattcaac ataaataaat ttttaaatta agacaccata
    74761 ttgactattt cattttatca acatattcca tctttgtcaa tatattgtct tttttagtaa
    74821 tttaaaatta tgtagtctat atgaaatcca gttgattttt ggctaattaa agataggaat
    74881 gctattaggt ttaaaatata tctattcaaa ataaaaaacc actcatttta agaatggttt
    74941 ttaatataaa ctatttgtta ttaataagga tattttttta tttaaattta tataatcgaa
    75001 catctaatat ttaatgttta ataaaattat gcatttgcaa aagtatatat ggcattaact
    75061 cttgctacat ataaattctc atcactacat ccaatatatc tagcatattt ttcaatcttt
    75121 tcttttgcat tttcaacatc attttcatcg ctctccatta actcatttat ataaatatcc
    75181 attaatgacg ctttatattc tcttatatca tagcctaaaa attcagtcaa tctctcaatt
    75241 tccttactaa aatctccatc ttcttttacc aaaaattcgt atacatcttc cctatatgga
    75301 tttaaagtta ttatttgttt tactactttg cattcgtcag ccttcccaat aaaaccatct
    75361 ttataattat taaataaggc attagatttt tttatagatt cttcatcaat ataagaatgt
    75421 accgcagtta tacgattttc tataagggca tctacaacag cataatgaat tttaaaacaa
    75481 tcattgtata aataattgct cattttcttt attgtcgatt ctatttctaa gaagctcgcg
    75541 tggccatttt ttttattttt atttggtata ataaaagttt tattcaaatt attaaaaagt
    75601 ctcttaaaat ctaaatattt ttcacaataa acacttttaa aggtatcgat atctatagtt
    75661 ataattccaa aacttactat agtctcaatt cctttcttaa aagactcttc tatatatttt
    75721 tctcctaaac ttaaacctct atcagatatt tgctttagat tttgaaaact atttaaacaa
    75781 tctctttcaa agtttttaga tatttctaaa gcatataatt taaagatttt ccttatctta
    75841 ttataattat tttttgtatc atcaaatctg ataatagctc catttacatc aaaagtaatc
    75901 attgagtttt aacctcccca ttagtttcat caataaaata aatttaatat aaactttata
    75961 atcataatat taaacttatt tatactataa ttattaattt gatgataaaa gctattttcc
    76021 ttctaaataa aaaatttgtc taaagtttct aaaaaattta gacaaatctt aggttattac
    76081 tctttaaatt atttttaaat tattccaaat acgccaaata atattggtat aaatatagct
    76141 ggtaacaaat tggctacttt tatattcgtt aatttaagta catttagacc aagtcctata
    76201 attaataaac ttccagctgc tgtcatttct gtgactacag gatcacttaa aaatccattt
    76261 aaaaatgatg ctcccacaca tataatacct tcatacaaaa atacagatat tgcagaaaac
    76321 attacaccta tacctaaaga agcagtaaat ataattgaag ctattccatc tataacagat
    76381 tttacaaata aagtatcatg acttcctgta agaccacttt ctaatgaacc aactactgcc
    76441 atggctccta tacagaataa taaactagat gatataaaac cctctgctat agaatctttt
    76501 ttatttcctt tagaaaattt actttgtaaa aaagcaccca acttatttac ccatttgtca
    76561 atatctatta attctcctat cagagcacct acagctacgg atattattgt cactaatgtg
    76621 ttttttcctt ctagtgctcc tgatatacca atgtacaata cacataaagc aattccattc
    76681 attatagtat cgcttactct ttgaggtaat cctcctttta ttataagacc tactatacat
    76741 ccacctgcaa ttgctagact atttgctatt actccaaaca ttttttctcc cctcgattag
    76801 ctaattgaaa tttatcctat tattaaaacc taaacatcta tactttgtat tggatatttt
    76861 aaatattcca aaaattcttc aaaacctttt ctagatatat ttacagtttt agtatttatg
    76921 ttaggatgaa aatttaagta atcttcttta attacatctt tatctatgta aagctcaact
    76981 tttctttctg tatcatttat taatgaaaaa ggatttacac ttccaggaag aagtttaagt
    77041 acattgtata gtctttcagg tgaagcaaac gataatctag aacttcctat cttatcttta
    77101 agtgagttta aatcaacttg tttttctcct ttaactacaa taagataata cttatttcct
    77161 ttggcatttc ttaaaaataa atttttacat tgacacccct ttgttattcc atctaaaatg
    77221 actaattgct ctgctgtata tacagcttca tgctctacta catcatattc aacatttaat
    77281 ttatccaaaa tatcatatat tttagtttca ttcatgctag attcattttc atcttgttct
    77341 tttttattat ttaaatcact catacttttc tccttaaaca cttcatcata atatcattgt
    77401 gaggtattat tctactatag ctattgctct tactggagct ccatcagcat cattatactt
    77461 aaatggtgct gctataaata gaaatttttc tggaacattt tctaaatttg taagattttc
    77521 tatgattatt tttccttttt caaataaaat atgatgcact tcaaatactg atgtgtcata
    77581 tctatccact gatagcatat ctattcctat gccttttata ttagtattag ctatatagtt
    77641 tgcagcttct tttgttaatg taggataacc tacatagtac tgctttttat cccaaaattt
    77701 tgaccaacct gatttaaata ttataaaatc actatttttt atttcatctt catatttttt
    77761 tagatattct aattctatat tctcatttcc cttttcaagt tccaatacat aagctttccc
    77821 aacaaaattt tctatatcca acttatctag ggtctcacct ttagtataca tatgtcttgg
    77881 tgaatccata tgagttccat tgtgagaaaa tacactaatt aatgtttctt gataaccatt
    77941 ttcttcaata atagctactt tcttgatatc tggtctatta ggctctgcat atacaggcat
    78001 gtcattatgt gtcacatgag ttaaatcaaa aaccttcatc tttatatacc ccctatgttt
    78061 ttattttcta ttatatacct aaattcattt tatttctatc agattttatt ccattatata
    78121 ccatactact atatattttt tataaacctc aagggattta ccgaaaaatc ttggaaagaa
    78181 ttatcagtaa atataacatc ttcacccaaa aaacgtttat accttccttc ttctttcttt
    78241 aaagtaattc tctgtttttg tgaatgatta ttaaaaacca ttatcttaag aggaggtttt
    78301 tttctattag tctgcactct ttttaacatg tattttatgt gcatatcagc ttcaggaaat
    78361 gaatatccac aaaatattaa aaggtcagta tctcttaaag ttttttctgt ttcattccaa
    78421 acattactta aaaatatgtt tgacatattc ttaaaatatg ttggtggcac tataataggt
    78481 tctgttaact caccacactc taaacatttt gtctctgaac tattttcaat aagcttcatg
    78541 acaccacctt catgtggtgt aagagttaca ctattacaaa tggggcaata aagccaattc
    78601 aatgacccat gtatcttata taatttgatt aatggttctt ttggttttct ccaatttaac
    78661 tcctctttaa aatttgtaaa ctccacacca taatcaagca taattggcaa atcattctta
    78721 tctcttaatc cagtaactgt attgtctata tgtatatcat aatttgcact aataaaagta
    78781 gtatcaagaa gcaaatcatt ttccaaaaga ttttcaacta aaagtttatg atatttatta
    78841 tctgttctag gtgtattatg aattgcttca gccattagca gtattaaata ttgtcttaaa
    78901 aaacgtatac tatcacttct gttatttaga ttttctagtc caaaatttct aaatgcttct
    78961 tttctttgtt ctgctaagtc taaaagacct aaaacttctt caaaggtagg aaaatttgca
    79021 ttatctatat tatcttttac tatatcaata ttgaacatct gtttaaagaa aatatacaat
    79081 tctctattca ttttggtatt ataatttgtg ggatttagtt ttttaaaata ttcgctaaaa
    79141 agttcatttt gaataggtag attctctgct gctgaagctc ctgctcctaa aaatattgct
    79201 attttcatat agttcctcct aatataaata ttactcctat ttttatatcc attaaatatc
    79261 catatcattt attttatgat acaacaataa agctgtcttt tactagaaaa aatcctatta
    79321 caagacagcc ttatattata ttaaatcttt aataattcgt aaatagaaag agcatatatt
    79381 tgagtagctt ttataatatc ctctactatt aagtattcat tagcttggtg ttctgtttct
    79441 ggttttcctg gaaaaatagc tccaaatgca acacaattat ctaaagctct tgcgtatgtt
    79501 gctcctccag atgaaagtgg tgttccatca agaccagttt cttcttcata tacttttcta
    79561 agtgtcttaa caagaagagt atcttctgga acatagattg aatccaagaa atcatattct
    79621 tcataattta aattgtattt atctgtcatt ttctttaact cttttacaaa atcatccttt
    79681 ttgtatgtta ctggaatcct tacatctatt cctgcaaatt ctttttcgtt atcaatagtt
    79741 actctaccta tatttactgt tagtttacct gatacatcat ccttgcaatt aggaattata
    79801 ttgtttccat tagcatcttc accaataact tctgctaaaa acttaattat attagaatct
    79861 atacctatat tattaagagc tatacaaagt ctagccactg catttatacc agtatcactt
    79921 gctgctgaat ggacactttt tccaattata cagattttat taccttcaac agtatattca
    79981 aaatttaatt tatcaagttc tttttttaat ttatcagagt attttccaat atagattgct
    80041 ttacctggaa ctgcatttaa agcctttcca actgacaact ctatatcact tttgctatca
    80101 caagtcaaat gaacttgtaa aagacctttc tctgcatttg ttattgggaa tctagaatct
    80161 ggagtaaatc catagtttgg tatttcttca ttattttctt tatatttatt tatacatctc
    80221 caaagatttt cttcatcagc cccaaaaatg aatcttatcc tcttattaaa gtctacattt
    80281 aaatccataa gagctttcac tgcataaatt gctgatattg taggtccttt atcatcttgt
    80341 gttcctcttc catacaattt accatcttct aaaacagctt caaatggagg gtaattccag
    80401 ctttctaaat caccttctgg aaccacatca acgtgaccaa gtatacctat cattttttca
    80461 ccttgtccta tttctgcata tccataatat ccatctttgt atactgtctt aaatcctaaa
    80521 gatttacata agtctaatat acctcttaaa gctttatcta cattttcacc aaatgggcaa
    80581 ttttcagttg cttcagatat aacactaggt atttttacac tttcttgtat tgaagaaatc
    80641 atttcatctt gtagagaatt tactttttct ttaatttgct ctttcatatt atcacccttc
    80701 aattttcatc ataatatact aataaatatt ttgacctttt aaaaaaaaaa tatgcttatt
    80761 ttttcagttt ttttataaat tcaatcgctt ttaaagtccc catttttgaa tggtcaaatg
    80821 taagtccacc ttggaaataa acattataag gaggtcttat tggagcatct gcacttaatt
    80881 ctatagaaga accttgtata aatgctcctg ctgccattat gacttcactt tcgtatccag
    80941 gcatgtccca aggaacaggt tttacataag aatcgactgg tgcagcttct tgtatacctt
    81001 cacaaaaact tattacttca tcagcattat taagtctaat acattgaata atatcacttc
    81061 ttaagtcatc atattttggt aatacatcat atcctaattt ttcaaaagct cttgagcaaa
    81121 agatagctcc cattacagcc tgagatacta tataaggcgc caagaaaaaa ccctgaagta
    81181 catttctagt tgttccaaaa gtaagaccac attcttttcc tattccagga gaagtcattc
    81241 tataagatat aagctcaatc aaatcttttc taccagctat atatccacct gtcaatgcaa
    81301 gcccaccacc aggatttttt attaaagagc cagccataac atctgctcca acatcagtag
    81361 gctctttggt atctaaaaac tcaccataac aattatcaac cattactata gcttctggtt
    81421 taacagactt tattacttct atagcttctt ttatatcact aattgaaagt gattttctcc
    81481 aagaatatcc ttttgacctt tgtatcataa cgagtttagt tctatcattt attttgcttt
    81541 taattccttc taaatctaag tttccatcct ctaaaaaatc tacatcatca tatgtaacac
    81601 catactcttt caatgaacct ttttcttctc ttatgcctat aactccctct aaagtatcat
    81661 atggtctacc agtaacagat aaaatttcat ctcctggtct aactatacct tgtatacata
    81721 aagtaagtgc atgtgtacca tttacaatta ttggtcttac aagagcatct tctgtattaa
    81781 atactttaga ataaatttcc tctatttttt cacgaccaat atcattatat ccatatcctg
    81841 tagtccagtt aaagtgcata tcacttagtt tagattcttg catagctttt aaaactttat
    81901 attgattata ttctcttatt tcttttattt cttcaaactt atcttttatt tcttccatta
    81961 tttcttgtga tagtttaaaa gtatcatcat ctattccata gtagtctttt aataattctt
    82021 ttgtctcatt aagcatttta tcacctgtct ctttattatt ttatttaaac atgtaaagga
    82081 gactatttta ctaaaacaaa taaattatag tttataagtt agtataagtc tcctctcaat
    82141 catcttaaat taaattaatt atctatctgt tgttattatt attgttgttt tggttttgat
    82201 ttgttagatt aatatacttt ccaggttgta tagttgatac agcatgttta taaatcatgt
    82261 tttgttgtct attatcccct tctattaata ttatataact atcaaacccc tttacaagcc
    82321 ctttaacttg cactccatta actaaatata tagtaacagg tatcctttct ttccttgcat
    82381 tatttaaaaa caaatcttgt aaatttaaaa ctgtattttt cattagattt gtaccctccc
    82441 aagattaaat tgaacttcta aaatattcct accttatatt ctatataagt tatgacaaat
    82501 tttcaatatt aagttgtata cttaaaaatt tatatattat tttatagaat ctttaatata
    82561 taagattatt tcatttttta attcgtctat attttcatat tggtctaaat caaaccattt
    82621 tgctgtttta tatcttttaa accatgttat ctgacgtttt gcataccttc tagaatctct
    82681 tttaattatt tcaatagctt cttcgtatgt atattctcca tccagatact ttaatatctc
    82741 tttataacct ataccttgca ttgatatcat atctttttta aatcccatac ttaaaagttt
    82801 ttttgcctct tcaaccaatc catttttaat cataatatca actctcatat ttattctttg
    82861 atacaaatgt tctctatctc tatttagaac tattattatt ggttgatatt cttcattaaa
    82921 ttttaaatca cttgaaaaat cattcatttt cttaccactt agacaaactt caagagctct
    82981 tatgaccctt ttagtattgt ttttatggat tctattagca gattcttcat caagttcttt
    83041 taacttatta tgcatgtaat ctattccctt ttcagcaagc tgtttttgaa gttcttctct
    83101 taattcatta ttggcatcag attgtgcaaa atccatatta tatattaaag agtttaaata
    83161 aagtcctgtg cctccagtta ctaaaacaca tttttccttt tcatttattt catgtatata
    83221 tttttttgct ctttgttgaa actctgaaac tgaaaatgga tagtcaggct taacttcatc
    83281 tataaggtaa tgcttcactc cttgcatctc ttcctctgta acttttgcac ttccaatatc
    83341 catatattca tatatttgca tagaatcagc tgaaattatc tctgcattca tatcttttgc
    83401 taattttatt gacaaatctg tttttccaac agcagttgga cctgtaagta ttataagtgg
    83461 tatttttttc ataacattcc tttccatata ttttaaatat ccaaatattt tacataattc
    83521 ttttaaacat tttttctatt tctgtcttag atatttcgac cattataggt cttccatgag
    83581 gacatgtaaa tggattctca cacttttcta gctgttccaa taaacttttt atttcaatat
    83641 cgtatatttt atcatttgct tttatagcag acctacatgc catagatgca aatctctctc
    83701 cttttaaatc ataattactt gtaatttctt ctatattatc aattatttga agtataaatt
    83761 tctcagtttc aggtactcca aatatagtag gaacacatct taccattata tgattattgc
    83821 caaaaatttc tagctcaaaa ccaaatttca taaataactc caagttattt tcaatttgaa
    83881 gcatatctat atttgatact tcaataacca ctgggtctaa aagtatttgc atatttatat
    83941 cttgtctata gaacttctcc atatatcttt catatagtat tctttcatgt gcagcatgtt
    84001 ggtccaacaa atacatagaa tcgtctttgc tcaaaattat ataagtatta aatacaactc
    84061 caattacaaa atatccgtat aaagaaaact tgcgttttga actacacaag tattcttctt
    84121 cactgtcctt aatactatct cctaagtaat aatttccttc atttttaatt ccatcaactt
    84181 gaaactcttc ttgtatatta tcatttgcac tatcttcact tagataatta atgtttttta
    84241 catcatcttc tacacttgca tttaggactt cacttactga ttgaaattca tttattggtt
    84301 tttcacttga taaagtaaca acttcaatga cttcatcctt acctttagta atattactat
    84361 ttttaggagt agattctaat aaatcattat ttcttaattt ataatctgta cttttttctc
    84421 tactgttaat agcgattcta ggttgtgttt ttttatcact ataagtagca tattttccaa
    84481 taagatttga gtgtattaat ttaaccttta aaaaatctct aagttctata tacacttctt
    84541 gttctttttc aaatttaatt tcaagtttat tggggtgtat atttacatct atacatgatg
    84601 ggtctacttc tatatttaag aagcacactg catgtttacc tattggaatt atagacttat
    84661 aactttctgt tattgcatct atgattattt tactttttac aaatctttta tttatataga
    84721 tatgttgtaa gtttttattt gaacggtata tattgttatt acctatatat ccattcattt
    84781 taaagtgatt gcacttaaac tctacatcta ttatattttc cgttatttct tttccatata
    84841 tacttctaat agtgtttaca agttttccat cccctggagt atttagcatc tgtttattgt
    84901 tatttgtata tttaaattga atgtttggat tacctattgc aagtttattt atcaaatcac
    84961 ttatgtttat agtttctgca tgagtagatt ttaaaaattt ctgtcttgct ggtgtattaa
    85021 agaaaatatc ttttataatg atggtagttc cattagtaga acctattggt tctttcgata
    85081 ttatttttcc accttcaaca tatattttag ttcctattat ttcatcttta gttttagtcg
    85141 tcatttctaa ttttgataca gctgaaatag atgccaatgc ttctcctcta aaaccaagag
    85201 agtataaatc atacaaatca tcaatttttt taattttact agtagcatgt ctcaaaaatg
    85261 atttttcaac ttcacttgaa ggtattccta ttccattatc tgtaatctta attagacttt
    85321 ttcctccatc aattatatct atggaaattt tatttgcacc tgcatcaatg gagttttcaa
    85381 taagttcttt tactacagaa gaaggtcttt ctacaacttc acctgctgct atcttattta
    85441 tagtcaaatc atctaggatg ttaattatat ttttcatttt tacacctcta agattttatt
    85501 tcttttgctt ttctttgaag attataaaga gagtttatag catctaatgg agtcatattt
    85561 aaaatatcca gatttaaaat ttcttctgat agtatatctc tatttacact atcaaaagag
    85621 atttgagtta aggctacttc ttttacagaa tcttcttgat taattttctc tttatcatca
    85681 ttttgcttca ttaaagcaac ttcatcattt aatgctttaa atttagaatt taatttatca
    85741 taatcctttt ttatatgttt gtagtctttt actataagtt catgttctat ttttagagat
    85801 tcatatttat tcttaaaatt actttgattt accttaacta attcttcgtc caaattacta
    85861 ttaattatat tattgttttc tttatctcca tttattgcaa cactgttgta aacatgattt
    85921 ttttccaagt ctttaagtat atattttgct ctttctatga cttcgtctgg taatttagct
    85981 aatttagcaa catatattcc ataactcttg tctgctccct gaggaattat ttttcttaaa
    86041 aatattattc cttccccatc ttctttgact gctattgaat aatttttaac ctctttaaac
    86101 tcttcttcta aatcagtaag ttcatgataa tgtgttgcaa ataatgtctt acatctaata
    86161 tttttttgaa tatattccac tattgaccat gctaaactta taccatcata ggtactagtt
    86221 ccacgaccta tttcatctaa tataactaaa cttctctctg ttgcattttt taatataagg
    86281 gatacttcgt tcatctctac cataaaagta gattgacctt gagataaatc gtcactagct
    86341 ccaactcttg taaatattct atctaatatt ggaatatcag cagactctgc tggtacaaat
    86401 gagcctatat gtgccataag agctattata gcagtttgcc tcatatatgt tgatttacct
    86461 gacatattag gaccagttat tatgttaata atgttttctc cacgatttaa ataagtatca
    86521 tttggaacga aattttcctc tcctactata ttttcgacaa caggatgtct accatttctt
    86581 atatcaagtt tattattttc atttattgct ggttttacgt agttatttat atgtgcaact
    86641 gttgcaaggg acacaaaaac atctatatta gctatagtct tagccacttt ttgtattcta
    86701 tctatgtttt tatatattgt atctcttatc tctacaaata tctcgtattc taaagattta
    86761 atcttttcct ctgcatgtag tatcttttct tctatttcct ttaattctgg agttatatat
    86821 ctttctgcat tactaagtgt ttgctttctt atatatgtct catctagctt tgcctgttta
    86881 aagttggctt ttgttatttc tatataatac ccaaaaactt tattaaatcc tatttttaat
    86941 gatttaactc ctgttttttc tctttccctg ttttctattt cctttacaag aaaggctcca
    87001 ttcttagaaa tctctcttag ttccttaagt tcatcactaa aatctgattt aattatgttt
    87061 ccatctttta tggttatcgt aggttcttct agaatagctt catctattaa attgtatata
    87121 tcatctaatt tatccatttc tgaaacatac tcttttagga tttttgcatt tgataaattt
    87181 atagtatctt ttaaattagg taacttttct atcgaatttt ttaagtgaat taactctttt
    87241 ggtgttactc tctcaaatgc tatctttcca catattcttt ctatatcgta tatattttta
    87301 agtatatcat ttaggtcttc tcttaatata aggtcatctt ttatttcttc tattacatct
    87361 aatctatttt caattttaga tttatttatc aaaggttctt ctacatattt tcttaaaagt
    87421 ctacctccca ttgctgtaga agttttgtca agtacatgta ataatgaacc ttttttctta
    87481 ttccctctta tagtttgagt aagttctaaa ttagttctag taaacatatc tagaaccata
    87541 tattctgatg aattgtatat atttatatta ttaatgttag atgttatttg tttttgagta
    87601 ttgtatatat aattcagaag aattgataag cttgacttta ttagaccttt atcatcaaac
    87661 tttagttttt gtagataaac atctggaaaa tattctttta atatatttat atccaaatag
    87721 ttatcactaa aactttcatt tatgtatata ttacttacag tagctatatc tctaagcttt
    87781 tctataaagt ctaaatcatt tataattatt tcagttggat ggatttttgc aatttcttca
    87841 attaccttat cttcatttaa acatgttgca ttagtttctc ctgtacttat atcaacataa
    87901 gttagaccta tattagtacc atccttatac aaagataata aataattatt ctttttattt
    87961 tcaagtaaat ttccatctaa aactgtacca ggagtgatga cccttataac ctctcttcta
    88021 acaattcctt ttgctgtaga tgggtcttcc atttgttcac ctattgctac tttatatcca
    88081 ttttcaacta attttgatat atatgaattg gctgagtgga aaggaacacc acacatagga
    88141 gcacgttctt ctaaaccaca agcttttcct gtcaatgcta tttcaagagc ttttgaagct
    88201 actaatgcat cttcaaaaaa catttcataa aaatctccaa gtctaaaaaa cagtatacag
    88261 tctttatatc tattttttac ttcaagatac tgtttcatca taggagttaa tttattcata
    88321 tctatttgca ttatcaaacc tccttctttt ctaattaaat attatatcat agtagatgat
    88381 tattattaac acaatatata aaattttgac atataactaa aaacaatcca aaatgtatac
    88441 tcacattttg gattgctaag tttaatgctt attaaataaa aatatttttt tttacaataa
    88501 agatattatt tcttttttaa gtttaatgat attttcaagg ttatcttcat ttaactttct
    88561 actatctata acaaattctt tctttactat tgatggtttt tttgaaataa cataaattct
    88621 gtttgatagc attatagctt cgtcaatatc atgagttata agaagtattg ttgaatttac
    88681 ttttttctta acatctaaaa gccatttttg catggatgtt ttagttaatg aatcaagtgc
    88741 cccaaaaggc tcatctaaaa gcattatgtc attagaattt ataaaagttc tcaagaagtt
    88801 agctctctgt ctcattcctc cagaaagttc tgaaggatat ttatcttcat atccgcttaa
    88861 tccaaacacc tcaaaataag gttttaccat tgacctagct ttactctttt tttctttttt
    88921 taaaattagt ggtagtgaca cattatctat tatagtttta tatggcaaga gtaaatcttt
    88981 ttgatacata taactatact taccatttat attcactttg ccactatcat aatctgtaag
    89041 tttagttata atattaaaaa tagtactttt tccacatcca cttggcccta atatagaaac
    89101 aaactcacct tcataaacat caatacttat atcttttaaa acttcaatat ctttataagt
    89161 ttttgataca ttttctatag atacttttac ttttttattg tggtaaaaat tcatttgtgt
    89221 aagcatcact tgccttcata tccttatcta ttaatttata ttcttttaag aatgatgtat
    89281 agttgttcca tacactatct ttcatttctc cccatcgtgg tgcatcatct ttgtacttgc
    89341 ttgctaaata ttcttgactc tttaaagcta gtttttcatc tacttcaggt gcatgtttta
    89401 ctaaaatttt tgctgattct tctggatttt ttattgcata ttcataacct tttgttgtag
    89461 cttttaagaa ttttttagca agctctggat tatttttaag cacagtttca ttagaaatta
    89521 taagtggtgt ataataatcc aatctctcat ctaaatcttt tactggtata aagtttaagt
    89581 catatccaat taaatcagct ttaactgcat cccaaccttc aaatatccat gcaaaatcta
    89641 cagttttcat agctgcaaag aaatcatctt gaccagtatt aactatcttt aacttattaa
    89701 aatcagctcc atttttcttc ataactgctt taaaaacagc ttcttctgat ggagagcccc
    89761 aaccaccata agtttttcct tcaaaatctt tagctgttgt tatattcttt tcttttggtg
    89821 atgcaaaccc agaagtatta tgttgtataa cagttgctac agcttttatt ggaagtggat
    89881 cttcactagt ctttgcataa gtaacttgtt cttgatagct tataccaaag tcagcttttc
    89941 ctgttgctac taaagtttct gctccacttt caggtggttg tactatttca acatccaaac
    90001 cttcttcttt atagtaaccc ttatcaagtg ctacaaataa tccagtatga tttgtatttg
    90061 gtgtccaatc aagcaccata gttacttttt tagaagaagt atctttatca ttgtttttgt
    90121 ctttattgtt atttgaagaa catccagtta ttgttgccaa tgtaaaaaca cttactaaac
    90181 ctaatattcc taacttttta aaattcataa ttgcctccct caaataccct taatctatta
    90241 atctatgtct tataacaact ttttttacag cctgtacaat tcctacaaac attaaactaa
    90301 atataactac taatatagta gatgcaaata ctttatcaag tgcatatgca ctttttgacc
    90361 taagcatata aacaccaagt cctttcgttc cacctagcca ttctgcaaca gttgctgcca
    90421 ttacagcata cgttgcagat attttaagtc cagaaaaaaa tttatccatt gccataggaa
    90481 atttaaggtg aatgaatgta ttaactttat ttgagtccat agtcttaaat aaatttaaat
    90541 aatctttatc aatattctca atgccatcta caaaacttat taatatagga aaaaaacaag
    90601 taagcattac catcaatact tttggcattg ttccaaatcc aaaccatatc ataaagagtg
    90661 gtgctataat tattgtaggt atcatctgtg taaccaacat aataggataa agacattttt
    90721 ttattattga tacaaaatcc atcaatattc caattaccaa tgatattata attgcgacta
    90781 taaaaccaag tattgcttct cctatagtaa ccacagcatg tatggataaa ttttgataat
    90841 ctttaaaaaa cacattaatt atatctactg gtgtgggtag tatatactga ggaacttcta
    90901 aaactcctac catagattgc caaagaataa gtataaacaa aaatgttgct attggatata
    90961 tactatcttt gactttatct atgatttcta agtttttcat ctatagtcag acctccagct
    91021 ttttcatcta ctctagctac tgttatgact ctttcacatc caagattaaa agggatttta
    91081 tgtacctttt ttaataattc aaaaacttca tcaaaatcac cttcaacagt agtgctcatt
    91141 gctcctattt cacatttaag accacttttt tcaatttcag caatacaagc atctactact
    91201 ttatacatct cttcttcacc tttgtaaggt cttagtggca ttactgctat gtctgcgatt
    91261 accatttaaa tcactccttt aaaaataatt aatacctact aggtatattt tgttttttat
    91321 taaaaaattt tattactaaa aagatattat atttaaataa aagaaaagtc ttatccaaaa
    91381 taggataaga cttattttta tcttaactgg tctccctaca ctggcattat ccaaacaggt
    91441 tcaaagggtc gagataaaat ctcctctcag cacattagct ccccgaaata tattatatct
    91501 tttctacttg attatactac acttatcata ttttaacaaa ttttttattt atattagata
    91561 ttttatccgt tcaaaaatat ctaatataaa ttgtacatta ttacaccaaa taaattatct
    91621 taaaatggta atttatcaac atcttccaaa actagattaa ctgaattttc tagtttttat
    91681 tttgctattt caagcatatt tggatgtata tcaattccat acaaatattg tgctaaattt
    91741 ctgttttcac tcatctaatt tgcaatcgtt acaaattaca gattagaaat agcttttaga
    91801 ctctacttat ctttatctac aaaaaatcct tcaataaatt cattaataat attcagtaat
    91861 ctaaaaataa acctctatct agatttttaa gatacaagcc ttttaggaag tatcttttgt
    91921 gataatataa aaattactca tcattatcat caaaatcctc agcaaccaac attttagata
    91981 agaatcaaac tctataatat aattatttta tccaatttat ttttgagaaa taaaatattt
    92041 ttttaatttt taaatttttt aatatgcacc ttgaaaaaag aaacgttttc ctatattatg
    92101 gtaatagtag aataaaacta aataggcaaa actagagaaa tttagtgacg caaagctata
    92161 gggactaaga cttatgaaat gagttatgtc agccagttgc caaagagata ttttcttttt
    92221 gtttttatga atgtttttta gggggatata tgatatatgt ttatggataa aatggataga
    92281 tgtacacata tactaactgc atatatatgc agttcacgcg attactgcaa ttttatagat
    92341 acacaattaa atgattttat actagaatat ggagagaatg tggtagaatc ttgtttgcat
    92401 caagtgatgg tattggtaag cagatataat taaagaaatt ttattttcta gaaaaccctt
    92461 tttagtttta tctacgtttt ataattcatt aaagaactaa ttaaaataag cttcattata
    92521 tatttttcat aaagtaaaca gttgagttta tctcatatac tgtttacccc aattacataa
    92581 ttctaataat atagattcta atgatttacc acgtttagta gacaaatatt caacttttgg
    92641 aggaatagtc ggataagaaa tcctcttaat aagtccatct ctttcaagat actttaattc
    92701 ttgtgacagt atcttatgac ttatatgaac aacttctttt tttaattgac tgtaacgtat
    92761 tggctcttgt tttaaaatat gccataaaat aattgccctc catttacttc ctatcacagt
    92821 catggtaaaa ccaattgaac aattaaaatc tttttgtctt tcttcaatat ttggaaattc
    92881 ttcaaaatta ttatctatat ccatctcaat cctctctttc aaaatagtaa gtatctaact
    92941 aaaaagtgca ttcttgttta gcgtactaat tcgtaatata atcttgattg tagtatagag
    93001 ccttacataa tactactttt ttagatgtat atgtttaaac tttaatatag acatatgtta
    93061 ttttattaat tttatttaaa cagataataa taaataataa tttatctcaa gttttctatg
    93121 tagataaact aatctaatgt ttagaaagga ttatataata tgaataaaaa atttcgtctt
    93181 tatcaagttg actcattaca aaagaaaaat ttacaggtaa tccagctggt gtaattacaa
    93241 atgctgatgg cttatctgaa tcacaaatgc aaaaaatagc aagagaatta aataattcag
    93301 aaactgcttt tattttcaat aacttggcta aaaatcatca agaaaattat gatgtacata
    93361 tcagattttt tacactaact aatgaggttc ctatatgtgg tcatgctact attgctgccc
    93421 attatgttag agcagtagaa aaccaattca ataacaaaac agtatttcaa aaaactagtg
    93481 ctggaatatt accagttgat attataaaaa aagataatga ttatgaaata attatgacac
    93541 aaggaaaaat tgaagtatct ccaccgttac aacaaaccta tcaaaaaaga ttacttaatg
    93601 catttggtat tttaaaggaa gatattcgta cagattgtcc tattgccata tcatctacag
    93661 gacattcaaa agttatgatt ggaataaagg aattatcaac attaaatagt ttatctccaa
    93721 atttatatga attatcgaaa ataagtcaag aaataaaatg taatggatat tatattttta
    93781 cattacataa taaagaaact ctacaagttc atggaagaat gtttgctcct gcaattggta
    93841 tttcagaaga ccctgttaca ggaaatgcta atggaccctt aggtgcgtac cttgtacatt
    93901 ataaagtaat acctacaccc aacacagagt ttatatttga agcagttcaa ggagaaacaa
    93961 taaaacgcaa aggcaagatg ttagtaaaag taattatttt agataataaa ccagttgaag
    94021 taaaaataat tggaaaagct gtaattgcat tctcaacaaa catagaaatc taattataga
    94081 ttccttctaa gtaaattttt gaacttttta atactaattt aataacaaac taatttatat
    94141 acaatcaaat taattttttt attatttgtt aatatatatt tatcattata ttatataata
    94201 atatagatag ttactatatc ttataattag ataaatcata attatatatt attgttaaaa
    94261 ctcaattata taatttttaa taatattatt ttaggaggta aaaatgataa acgatattaa
    94321 aaataaattc aaaaattaca aaccatatat aaatggatac aagaatatga agagagcatc
    94381 tgttttaatt ccaatagtag aaataaataa cacacactat atattatttg aagttcgctc
    94441 aaaaaatttg aagcatcaac ctagtgaaat ttcttttcca ggtggtaaaa tagaatcagg
    94501 tgagactcca tatgaagctg ttattagaga aacttgtgag gaacttggaa cttttagtga
    94561 caatatagaa gtaatatcaa gtcttgacct ccttataaca ccagtaaagt ttatcataca
    94621 tccatactta ggatatataa aaaatattaa taatttaaat ataaacaagg atgaagttga
    94681 ccatactttt ttggttccta taaaatatct tttagagaat cctccaaata cttatagtaa
    94741 tactgtaaaa gtttttccag acgaaaatct accatatggt tcaattccaa accaaaaaga
    94801 ttataaattt gaaagtggaa gttatcctat tctgttttat aaatataaag attatgtaat
    94861 ttggggaatg acagcaaaaa tactagaaaa ctttttaaat tttttatcag aataacactc
    94921 ataataaatc atcaaaactt ttacataaga aatttataaa agtttctaaa tttaacaatt
    94981 ctaaaatatc aataactata ttttgtagca cttttttatt ttattattgt ttgaatattt
    95041 gatggttttc aatgcaattt caacataaat actatgtatt agtgtatact gttgatgaat
    95101 taaagtatga taatctattt aataaagtat gaagtaaatg attttaatgt gcctttatgg
    95161 taaaattact aataagtaat aaaattaatt aaaatatatg gaggtgctta taatgtttgc
    95221 tgtaggattt gttgttataa ttgcacttgc ctttttcatt ttaagtgtac tattatttaa
    95281 tggaaaagcc agtatgctac tagcaggtta taactctatg agtaagaaag aaaaagcacg
    95341 atacgataaa aaaaaattat gtagaagttc tagcattgtt acttttattg tctctataat
    95401 gctatttatt atggcgtttt tgggatatag aatagagagt ggtcaaatgg acgaaaaaaa
    95461 aatggttccc ttttctataa tatttataat agtcatactt tccagtgtat tttttaatat
    95521 cctctactct aacaaaaaat gtaaaaaata aatagttgta agttgagcag ttaattataa
    95581 aagattgact gctttttcat gtatattgac tactcttatt aaatatttta acatttaaaa
    95641 atctatgtaa aaataatgaa tatagtacaa acttctaata atgattttac ctttgtggtt
    95701 acagtccact atatataatt taatatgata acagggaatt actaatgaca actaggtaag
    95761 cttttataaa atataataaa acaaaaatag atgtacaatg aaattatatc ttatatcaaa
    95821 tttattttta ttaatagcaa aaacttacat tagcataaca tatgacaaat tcgcttaaaa
    95881 taaaaatttt taatataatt tgttctatag tacatctatt ttgattagtt tacataattt
    95941 tattattttg tacaatatta attatttact aatatcccat ttaatgaaaa agttctaggt
    96001 tcagtaattt ttaccataac taatttacca attaaatcat catttccacc ttcgaaattt
    96061 acgagtttat tttgtctagt tctaccagca aacttatttt cattattttt acttcttcct
    96121 tcaactagaa cttcaactat tctatcttta tatccatcat ttatttctgc agaaatccta
    96181 tttacagctt ctaacaccct attaaatcta tcatgcttta tatcttctgg tatttgattt
    96241 tccattttag cagcaggagt tccctgtctt ttagaataaa taaatgtaaa agctgaatca
    96301 tatctaactt tttcaaccac atctaaagtg tccaataaat cttcttctgt ttctcctggg
    96361 aaaccaatca ttaagtctgt agaaaaagca atacctggaa cttccttttt agctttttct
    96421 attatttcta aataatattc ctttgtataa tgtctattca tttttttcaa aaggctactg
    96481 cttccacatt gtattggtag atgtaaaaac tcacacacct tatcacaatc tctaattgcg
    96541 tatattactt catcagatat atcttttgga tgagatgtca taaatcgaac cctttcaatt
    96601 ccttctatat cattcacttt tcttaaaagt tctgagaatg aaactggatt ttcaagtgtc
    96661 ttaccataag aatctacatt ttgtccaagt aaagtgattt ctttcgttcc atttgcaaca
    96721 agctctttta tctcgttaat tatatcttca ggagttctac ttctttctct tcctcttgtg
    96781 tatgggacta tacaatatgt gcagaaatta ttgcatccat acattatatt tacaaatgct
    96841 tttaattcaa attttctatt acttctaaga ccttctatta cttctccatc cacatcccat
    96901 acatccacta acattttgtc agaatttatt gattctgtaa gtaattgcgg aaatttatat
    96961 agattatgtg ttccaaatat aaggtctaca tgtttatatt tttttcttaa ttcctctaca
    97021 acatgtggtt gttgcatcat acatccacat acagctattt tcatatctgg attttttcct
    97081 tttagatgtt ttaattgtcc taagttacca tacactttta gctctgcatt ttctctaact
    97141 gcacaggtgt tatatattat taaatcactt tcttcaacca tcattgacat ttggtatccc
    97201 atctcttcta acattgaaca taatttttct gaatcatgtt cattcatttg acacccgaag
    97261 gtttgagtaa acacgagttt tctctttcca gttctgttga agtattcttc attttgtctg
    97321 tgtatttgtt ctatatattt atcttgttct tgtattttct ctataggtat tgttacttgt
    97381 tttcttttgc tcacgtctat tcctcctagt taatttctta tattacacat aaccttaatt
    97441 atatcaaata agaaaaatct tttaaagata ttattatttt tctagttgtg ttttaatagt
    97501 aaatctaatg atttatatat actatatttc taaaatataa aatcatataa ttattataat
    97561 aataaaaaga tgttttaaat taaaacttaa aacacctttc cttaaataaa atatatgatt
    97621 tactattaaa tacaattaca tctattcatt ttcaattaaa taacaatcat ttaatattaa
    97681 atattgatct tcctcaaccg ttatatatct tcttcctgaa aaagtatcat tagtaactat
    97741 tccctctgca ttacctaagc tatcatttct aacttcataa tatgcatcag aagaatttga
    97801 cattataata tattctccag caggaatttc taatccaatt ttatacatac catttccaaa
    97861 cttaccattc ttggccttct gggctttagc atactttgct aatgtcattg ttgaatcctc
    97921 aattttaata tattgaccat tctttaaggt gatatatcta gtcccactaa aaatatcatt
    97981 tgaaagtata ctatcagcat tacctgtact atcacttgta acttcataat aagaagcata
    98041 acttcctgag tttgaagtta ttaagtattc acctgctgag atatcttttc caaccttata
    98101 cattccatct tttatagtct ttgtagtagg tttaacattt tttacaaccc cagtattaat
    98161 atctccaatt ccatttgtta tatttaaaca ctgttgtact atatttgaat ctatatatcc
    98221 tatagaagtt atcttcttag cattttttaa aatgctttta ttgctaccat cgttaactaa
    98281 aaccattgga ccatgttttg ataaagaaga tcctacaagt gcatttgtta attcataagc
    98341 tccagctata taaaactctc tagcatcctt ataaaactta tttattattg ctttattagt
    98401 ttcaaaacga gtactccccc ctagtctagt agatttagta ttgtttacta aagtggtact
    98461 cattgaagct gttcctccta ttacataaga ctttaaacca ctagttgaaa aaggtatact
    98521 ttgtccatta gtaagaatta ttggtgcttt atctcttgct gctacagatg caatacttat
    98581 agcatctgct tctcctttat atgcattggt tagcataaca gtattgactt tttttattga
    98641 atttatttct tttgcaacat tataacttgt ttttattctg tcatttccct taattcttac
    98701 aactttcatt ttcttagatt ttaatgaatt ttcgacagat gtactaatag aatatgttcc
    98761 tccaattata taaacttttg atacattttt taatcttgca gatgtctcag ttggtatagt
    98821 atttttttca gttaatagta taggggcatt taaagctcct gctagtccac ttgcacttaa
    98881 tccatctgca atagagttac tagtattaat taatatagca gttttatatg cagttttata
    98941 tgcagtttta tatgcagttt tatatgcatt tttatctgct attttacctg ctatttcata
    99001 cttatttttt ccttgtattt tttctacaga tgatattgca tctactaatt gaatatttgc
    99061 aaatacaaaa gaaatactta aagtcaatgt taatattctt ttatagaatt tcataataaa
    99121 attgtccccc tcataataaa tttttaatat ataaatacat attcttaaat tagttatgta
    99181 cttaacaaac ttatgtaaaa ttgcaaatta tattaagtat attacaaata aatattaata
    99241 caattattca tttagctata attgtattta catttatatt aaaaatcaac tagatttgta
    99301 taaatactta taattatatt tttattctta atattttttt tattatgtta ttttaataga
    99361 tttatttttc ttttttaagc atagagatgt atttttccat atattctatt tctattatag
    99421 tttcgcttat ttaattcacc caaaatttca cttaatatta gaattttaaa tcttttacta
    99481 aaacagactt ttatagttct attaaaattt tttactaaac tttatttttt a
//
LOCUS       DAGSRM010000004        91833 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.004, whole genome shotgun sequence.
ACCESSION   DAGSRM010000004 DAGSRM010000000
VERSION     DAGSRM010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 91833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 91833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..91833
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.004"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            39..155
                     /gene="rrf"
                     /locus_tag="MJE33_000286"
     rRNA            39..155
                     /gene="rrf"
                     /locus_tag="MJE33_000286"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            275..466
                     /locus_tag="MJE33_000287"
     CDS             275..466
                     /locus_tag="MJE33_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBY9646267.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMVSRIIYF
                     IVGLAGLYLIPSLMSDDRE"
     gene            621..2030
                     /locus_tag="MJE33_000288"
     CDS             621..2030
                     /locus_tag="MJE33_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBY9646268.1"
                     /translation="MFIINELNKIVNEEVISFHMPGHKKGQVYKKLGYKDILKNLYKM
                     DTTEILGTDNLHFPEGIIKNSQKKASRVFKSDYTCYLVNGSSCGIQSAIMSVCSPKSK
                     IITNRDCHQSVINGCILGDIDIEYVPCEISEETNVLKGISISNVIDIIDKNLDAKAVL
                     LTYPTYYGMTYDLEYICNYAHSKKMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQS
                     THKTLPAFTQSSMIHIQGDRVDIDKILNMLRITESSSPSYLLLSSLELAVDIYENKGK
                     ELMEELLNNIITFKNKVDSNINIYDTNDKTKIFISSKNMGLTGYELENILRNKYNIQV
                     ELSNYYGVLLICTIGNTVEDFSSLETALNNIVKEDFKFEKLENKKYPVVIPKKILTPR
                     EAFYKTKKSVKIYDSIGKVCGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIV
                     SGLKDVDLNFIQIIDLEDI"
     gene            2161..2850
                     /locus_tag="MJE33_000289"
     CDS             2161..2850
                     /locus_tag="MJE33_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBY9646269.1"
                     /translation="MKGKLIIIESGSDASGKATQTEILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIIIADRY
                     TTSNMVHQASKMDICDREEYLNWLADYEFNLFKIPQPDCVIFLDVPIEFSKKLMENRK
                     NKITGEEKKDIHESDIEYLEKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKPMER"
     gene            2853..3788
                     /locus_tag="MJE33_000290"
     CDS             2853..3788
                     /locus_tag="MJE33_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBY9646270.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHKDYKIYIINNAEK
                     MTVEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKRYLI
                     NTGIEEERAQLLAIFSRGSIENALNLSQSSEFSMMREDIQQYIQIMLDKNIVEILNIP
                     NNMEKYRGKIIALLDMMINYFRDIILLKENVNKNMLINVDKLVFIQNMSGKISYSQLS
                     KIIDIIEDAKSKIKSNCNFNISIQVMSLNIYEVIK"
     gene            3785..4678
                     /locus_tag="MJE33_000291"
     CDS             3785..4678
                     /locus_tag="MJE33_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBY9646271.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVYLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            4756..5502
                     /locus_tag="MJE33_000292"
     CDS             4756..5502
                     /locus_tag="MJE33_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBY9646272.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSIKLNGLEERVEIINED
                     IKYIDKVLDINGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRAASRLVM
                     PRGKFFMIHRPTRLVDIITLGRKYKLEPKVIQFVHPRPQKAPNLVLVQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNKDIGEL"
     gene            5503..6336
                     /gene="rsmI"
                     /locus_tag="MJE33_000293"
     CDS             5503..6336
                     /gene="rsmI"
                     /locus_tag="MJE33_000293"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBY9646273.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRTLKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGDYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHRFVFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMLKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEVRGEFVL
                     IVEGFHGEKSKKENYDGLTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            6359..7063
                     /locus_tag="MJE33_000294"
     CDS             6359..7063
                     /locus_tag="MJE33_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBY9646274.1"
                     /translation="MEIGKIIIESLKREDTHKIFIELDNKHELEKIIPKISCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNDKDFFPIHLRKHVSNYLNTYIEEGINKLHVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGIKLELSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSGEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(7251..7679)
                     /locus_tag="MJE33_000295"
     CDS             complement(7251..7679)
                     /locus_tag="MJE33_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBY9646275.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(7716..9146)
                     /locus_tag="MJE33_000296"
     CDS             complement(7716..9146)
                     /locus_tag="MJE33_000296"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBY9646276.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLEYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGRCKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            9278..9862
                     /locus_tag="MJE33_000297"
     CDS             9278..9862
                     /locus_tag="MJE33_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HBY9646277.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMRRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSMQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTAVAIITCKFFQRRYR"
     gene            9878..10399
                     /locus_tag="MJE33_000298"
     CDS             9878..10399
                     /locus_tag="MJE33_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HBY9646278.1"
                     /translation="MEIFSNLLIPIIILYIVVYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANMMSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     misc_binding    10465..10711
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10795..12732
                     /gene="metG"
                     /locus_tag="MJE33_000299"
     CDS             10795..12732
                     /gene="metG"
                     /locus_tag="MJE33_000299"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBY9646279.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFKKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTNFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDETKKDELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKIISCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     gene            12762..13532
                     /locus_tag="MJE33_000300"
     CDS             12762..13532
                     /locus_tag="MJE33_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBY9646280.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIETSI
                     TAIELAKKYDFIYSAVGVHPHDVSKLDDTAIETLRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKNPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGISYEEVCRVTKENAKKFFNIK"
     gene            13869..14300
                     /gene="phnG"
                     /locus_tag="MJE33_000301"
     CDS             13869..14300
                     /gene="phnG"
                     /locus_tag="MJE33_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HBY9646281.1"
                     /translation="MYTVDSTKILINMNLEKLEKIYSMIKDTSSISIIKEPNLATVMV
                     RANESVKNTTFNLGEILVTECSVKVDESLGYGIVSENNNKKAIYLAVIDAVLHSNNSK
                     FDELKNYINKSVYEENLRYEQEIIDEFSLINKTKVQFNAMD"
     gene            14315..14890
                     /gene="phnH"
                     /locus_tag="MJE33_000302"
     CDS             14315..14890
                     /gene="phnH"
                     /locus_tag="MJE33_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HBY9646282.1"
                     /translation="MVNYTQEIYRKMLDSASFPGKLNNIDNINIENNTKLLNGAIGIA
                     ITLLDQEVTFYIENYTQKDIKNIRALTMSNQIDYRKSDYIFLDINSEVDILQIKIGSF
                     EYPDESATIVHQVNDLSTYSQTKYIKLDLSGPGIKSKNSIYINGIREEFIERLSSINK
                     DYPIGIDLILVDKKGEIAFIPRSSKLSWEVL"
     gene            14893..15978
                     /locus_tag="MJE33_000303"
     CDS             14893..15978
                     /locus_tag="MJE33_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HBY9646283.1"
                     /translation="MAYVAVKGGEEAILQAKNILEFYRVKGDSLPIDVKQIEEQMSYA
                     VDKVMSEGSLYNKELSALAIKQAQGDILEASFYVRALRSTMPRITYSEPINTEEMRIK
                     RRISATFKDIPGGQYLGATRDYENRILNVELLEEDEKKNRHFRDSFINELETVDEIPE
                     FTKISNIFRKENLLQEEVCENKLEEVIDDITRNSLRFPVSRSAKLQALTRGESGVVSG
                     FAYAIVRGFGDEHPYISELRTGNVFIKVKHPIYEDEEIIIGDMLVTECEIVSKASQNK
                     TENSKTNSEDKNKMSLGYGLCMGNNENKAISMAILDKSLEPNSEKLAQDEEFVLLHID
                     GIDSLGFISHFKLPHYVDFKADVERIK"
     gene            15998..16867
                     /locus_tag="MJE33_000304"
     CDS             15998..16867
                     /locus_tag="MJE33_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HBY9646284.1"
                     /translation="MIYNYGFLDENTKKEIRRKILKAISIPGYQVPFGSRELPIAKGW
                     GTGGLQLTLSLIGKNDVVKVIDQGSDDSTNACNIRSFIASVSNVETTKDTLEATLIQT
                     RHRIPEEKLKSNQILIFQVPIPETLRIVEPSEVETRRMHSEEDYSRMWVYLYEDIVRF
                     NDISIAVEYPCKVNDRYLMNPSPIPRFDIKKLNMSDNLFLFGAGREKRIYAIPPYTKV
                     EPLEFEDYKFEEEKFEGKYCSLCNSTNTFLDEVYDSDTNEKYYSCSDTSYCEKVRLKN
                     NSIDVTIGGTWNE"
     gene            16860..17726
                     /locus_tag="MJE33_000305"
     CDS             16860..17726
                     /locus_tag="MJE33_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBY9646285.1"
                     /translation="MSKINDSKRLENIDKQVLIGRNINLIYGDGCEKCFESTGYESNN
                     ICKYCGSIVACNNVNLELYEGEVLGIVGESGSGKSTLLKILFFQEHANSGEVYISHYN
                     KDTNILELSDQKKRYIKNHFMGIVYQNPHLGLNLDFSSGGNIAEKLLMANLYNVEKIR
                     DRGKELLIKTNIPVERIDHKPKYFSGGMQQRVQIAKALSNNPPILLLDEVTTGLDVSV
                     QAEVLDLIREIQRELKISMIVVSHDFDVIKMLADRTIVMKNGSVIESGLTDQIMEDPQ
                     HPYTQQLINSLL"
     gene            17767..18480
                     /locus_tag="MJE33_000306"
     CDS             17767..18480
                     /locus_tag="MJE33_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBY9646286.1"
                     /translation="MKETILEVNNFSKDFELHALNKTITACSNISFEVSKGEFLGIIG
                     KSGAGKSTILKSIYKTYIPTTGEIIFNSEIYGNVDLAKIGDREMINLRKKEIGYVSQF
                     LKTLPRITAIELVVHSLIESGFEKDCSYDMAKDILNQFEIKENLWDAYPNNFSGGEKL
                     RLNLAQAMVKKPRLLLLDEPTASLDNQSKIYVKEKMLELKSQGTTMIGIFHDIEFMET
                     VIDKTFTMTKGTISESGVA"
     gene            18481..19650
                     /locus_tag="MJE33_000307"
     CDS             18481..19650
                     /locus_tag="MJE33_000307"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HBY9646287.1"
                     /translation="MIKLIHNANIVLEDMILENGHLIIEGDTIKKVSNDKIDYAFDFN
                     EIDEIIDAKNLYVIPGIIDIHSDAIEKEIEPRPSTLLPFNMAFYELDKKLPANGITTV
                     YHSISLGDGVGVRSIDNSLKMIKNIDSYKNIDSKSINHKVHLRYEVLYYEGLEKVLEL
                     LDENKIDYLSIMDHSPGQGQYTNPTFYKEYATKVWGVTENYVDTWLDDLVNLHDNLDW
                     NKIANIIGIAKTKNINVASHDDDTLEKMDFIKNIGINVSEFPITLEVAKHSKKLGVLT
                     CLGAPNIVRGKSHNNNLKAMDAILEDCCDIVCSDYLPSAMIKSMCIVAERINNLNKAV
                     SLFTSNPAKAVGIYDERGSIKENKKADLVLVDIDSEYPKVVNTIVNGKTVYKREV"
     gene            19654..20490
                     /locus_tag="MJE33_000308"
     CDS             19654..20490
                     /locus_tag="MJE33_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBY9646288.1"
                     /translation="MLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEALVLGEHYGITIIPGIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSNLYKELFH
                     RGNGKVYVSLKYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLSYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            20491..21486
                     /locus_tag="MJE33_000309"
     CDS             20491..21486
                     /locus_tag="MJE33_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBY9646289.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKEVLEVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDDGINKIAIRPEDIKVESNRDSKDKDIKVGEILDIE
                     FRGFNYRITVEYRSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            21504..23192
                     /locus_tag="MJE33_000310"
     CDS             21504..23192
                     /locus_tag="MJE33_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBY9646290.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLVSLLKNAFM
                     DTSGNFIGISNFSKYVENPSLIASFKNSIFVSTVSSLISLCLAFIYSYAITRTNIKFK
                     EFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIVCSFITLLIFILIFVSVIGSVIKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAIFGTIFVFFSAYMIEKVDKFPKLRQTAYLLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNVVHFYSVCFITSNSSLKMLDK
                     EYELVAKSINIPFYKVFFNITVPMSITSILEIVVYYFVNSMVTVSALIFLYTPQTQTA
                     SISILKLDEIGYIGPSAAMAVLVLLTNIIVRLLYEFVTKKLKNNTQKWQQKDVA"
     gene            23204..24271
                     /locus_tag="MJE33_000311"
     CDS             23204..24271
                     /locus_tag="MJE33_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBY9646291.1"
                     /translation="MKFKKLVSLGVLSTLLLSVITGCSTDSSGSDSKSDSKTKGDLTV
                     YTAIEEDSIEPYLATFKEKYPDIKLNIVRASTGDITARLLAEKDNPQADVVWGVAATS
                     LLVADDQGMLEPYAPKGSEEIESAFKDDKEVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPSSSGTGYLTVSALIQIMGEEKAWQYMDKLHENIGVYT
                     QSGSAPATSAASGEYPIGISFGYRGIKLKEEGYPVEVVFPKEGSGWDIEANALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNYAVTTISTGNPIPEGFPKKPLEQMIDNDLKSAAK
                     NREDILNKWISKYDGKTEKES"
     gene            complement(24661..24825)
                     /locus_tag="MJE33_000312"
     CDS             complement(24661..24825)
                     /locus_tag="MJE33_000312"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646292.1"
                     /translation="MKSLLNSTLISSGLIATMFISSSIKSIVPIKSATPRANLNLNNS
                     SLVKYSYFQF"
     gene            25035..26102
                     /locus_tag="MJE33_000313"
     CDS             25035..26102
                     /locus_tag="MJE33_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBY9646293.1"
                     /translation="MKFKKLVSLGILSTLLLSVITGCSNVSSGSNLKSNNKTKGELTV
                     YTAIEEDSIDTYLATFKEKYPDIKLNVLRLSVGGITARLLAEKDNPKADVLWGVAATS
                     LIILDDKEMLEPYAPKGFDKVNSAFKDDKKVPSWVGIDAWMTGITVNTKELKAKNISA
                     PSSYEDLIKPEYKDLISMPSPASSGTGYLTVSELIQIMGEEKAWQYMDKLHKNIGVYT
                     QSGSAPAILAASGEYPIGISFGNRGIKLKGEGYPVEVVFPKEGSGWEIESNALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNFAVTTIDTGNPVPEGFPKNPLEQMIDNDLKSAAR
                     NREAILNKWIEKYDGKTEKES"
     gene            26294..26824
                     /gene="rnmV"
                     /locus_tag="MJE33_000314"
     CDS             26294..26824
                     /gene="rnmV"
                     /locus_tag="MJE33_000314"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBY9646294.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMERIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPQSIIA
                     ALNKVRTENTEKRDEFKQVDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVEREEFIDALKQI"
     gene            26995..27864
                     /gene="rsmA"
                     /locus_tag="MJE33_000315"
     CDS             26995..27864
                     /gene="rsmA"
                     /locus_tag="MJE33_000315"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBY9646295.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITRGDNIIEVGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLDNTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCDTEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EDIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREVLKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            27930..29192
                     /locus_tag="MJE33_000316"
     CDS             27930..29192
                     /locus_tag="MJE33_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646296.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKDDHGLIDLGNIILNEQGRGEFVLDLEDYDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEEYEELEEEDEEIEENEESDRDEDSEE
                     EYIIEEYDEDEYEDYWEDDEDIEEGELKARTEVEDSDDEEIEEFNNYKEIENYNRVKV
                     QEEYIDIEYDEEEEEEEVFEVNREEDRFVSSKPTKSKKVQKDQNNIEKIKRANGVSQG
                     ESGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMPYLG
                     YLNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGSTGFL
                     RYQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            29343..30458
                     /locus_tag="MJE33_000317"
     CDS             29343..30458
                     /locus_tag="MJE33_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBY9646297.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVVI
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEANGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYGKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKILGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIVAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            30644..31540
                     /locus_tag="MJE33_000318"
     CDS             30644..31540
                     /locus_tag="MJE33_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBY9646298.1"
                     /translation="METRYEEANKITIQSILWNIVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASVVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDENLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            31610..32407
                     /locus_tag="MJE33_000319"
     CDS             31610..32407
                     /locus_tag="MJE33_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646299.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITHGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(32462..33814)
                     /locus_tag="MJE33_000320"
     CDS             complement(32462..33814)
                     /locus_tag="MJE33_000320"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBY9646300.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
     gene            34033..34911
                     /gene="purR"
                     /locus_tag="MJE33_000321"
     CDS             34033..34911
                     /gene="purR"
                     /locus_tag="MJE33_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBY9646301.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDLIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
     gene            35067..35345
                     /gene="spoVG"
                     /locus_tag="MJE33_000322"
     CDS             35067..35345
                     /gene="spoVG"
                     /locus_tag="MJE33_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HBY9646302.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            35558..36937
                     /gene="glmU"
                     /locus_tag="MJE33_000323"
     CDS             35558..36937
                     /gene="glmU"
                     /locus_tag="MJE33_000323"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBY9646303.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEADVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVMIGNDTIIYPG
                     VMLQGKTRIGSDCIIGMNSSITNSEIGDGTEIKNSTIIDSKVGENSTVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SIVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITHDVPDGALAIARERQVIKEGWVEK
                     KNQKDDQSK"
     gene            37032..37982
                     /locus_tag="MJE33_000324"
     CDS             37032..37982
                     /locus_tag="MJE33_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HBY9646304.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
     regulatory      38820..38905
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            39067..39588
                     /locus_tag="MJE33_000325"
     CDS             39067..39588
                     /locus_tag="MJE33_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBY9646305.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSVKSAALSYYSDTNKIPVTPDGQTGLSVLETYMESLPDKADIGGKYKLIKVG
                     NKLVLQIGTNTEGVTLTEAQSAKLLSDIGENKIYTSTTTNSLGNPLTSNTKIDNNVLY
                     IVLIDNTVMDTTK"
     gene            39739..41415
                     /locus_tag="MJE33_000326"
     CDS             39739..41415
                     /locus_tag="MJE33_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBY9646306.1"
                     /translation="MAKKVRIGDKLVEKGYITEEQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIDVNESVTIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            41427..42635
                     /locus_tag="MJE33_000327"
     CDS             41427..42635
                     /locus_tag="MJE33_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBY9646307.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSIKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTTFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTSVGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILASAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISRQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVAILVVSIF
                     MPMLSIYDAM"
     gene            42650..44362
                     /locus_tag="MJE33_000328"
     CDS             42650..44362
                     /locus_tag="MJE33_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HBY9646308.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHDKDKFYVKNFNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSETPDNSDGDESSLNLIIGAIQKEEVNAIVEIMHEFDLVLDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDMQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQIKAKQMKNILK
                     IAPIVILILAIPYLCLIFSNNLANKELENVNGEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYDMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGTNNKADNSKSNKTTTDK
                     SSNNETKSSTKSDSNGDKTDNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFKDVKLHSIKSNAEDSNYTFSMTLYLKEGV
                     LTNE"
     gene            44355..45263
                     /gene="pilO"
                     /locus_tag="MJE33_000329"
     CDS             44355..45263
                     /gene="pilO"
                     /locus_tag="MJE33_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HBY9646309.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGILIKVIILLAILSYGYFGLYPQ
                     YQKYIATKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVKEYEHFYGISTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEIIKNDKLLDRSTNYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            45290..45856
                     /locus_tag="MJE33_000330"
     CDS             45290..45856
                     /locus_tag="MJE33_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBY9646310.1"
                     /translation="MSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKKQ
                     QIITDAQVNINLINKYLNRDLENCKELTKTGSGNNYEYNIEMPDNVVKYEVSIETKKN
                     TEVYSVTRIQNNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTGKSIYTVSIY
                     YNESVQESHKNSNLNNKTYTFDVMSRIG"
     gene            45893..46420
                     /locus_tag="MJE33_000331"
     CDS             45893..46420
                     /locus_tag="MJE33_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBY9646311.1"
                     /translation="MSKKNSERGFSLIEVLVAMAIMGIVLFAFFNIINTNNKANTKND
                     TDITSLNYVQSEIENLREKIKSGEFDFDSLDKLEDGTVVYEKLIDKSKKVVYDKVLSE
                     GDVSLYDTPYEKITTIKDEDGNLIDKGNITNKIKTIVEDKSGQIYKIAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            46441..47979
                     /locus_tag="MJE33_000332"
     CDS             46441..47979
                     /locus_tag="MJE33_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646312.1"
                     /translation="MRKWNKFKSERGAALVLVLVVVALLSIVGLIFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSIDKAYEKLYDELEKMDNEFKSENQEKSISRSKLRNISDE
                     DFNNQDEENIEAERLGYMNNINFYLNKASSDLEKASMELKKLYDLDMLDYRDIEYVDA
                     NIISHRDSILEICKNYTSGDISKINEYILKEDIDSTTLIEAKLVNNDILLKKFLEENK
                     IENEHLNSAFSHTYKALDNISLAMQNMIEYRHTFHIDEPKVEVSNGIPDSQQYYELIQ
                     NPIINSMEYSWNSKWDTLENLLEILPNQTQGFNSLRVHLRNNVRKFEKLSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEIDKTEGITIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKNGIKE
                     VEVTDGKKNIIGLGVEENSNSKYKVDAIVNFNLNIDTNVVGNYDIKDKILINHDISSY
                     KKVN"
     gene            48075..49157
                     /locus_tag="MJE33_000333"
     CDS             48075..49157
                     /locus_tag="MJE33_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HBY9646313.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTS
                     EVTMQMAKDIAGESMFKVIEEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPESIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKVGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            49138..49920
                     /locus_tag="MJE33_000334"
     CDS             49138..49920
                     /locus_tag="MJE33_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBY9646314.1"
                     /translation="MDIIIVNLYVFIVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLMSGRKCRYCYEKISSRYAIVELITGLLFLTVFMLYGISTSTI
                     YYLVLVSLLIIITFIDIDHFIIPDKILVFGAIFSMIFNLLFKEIPVKDSILGVFICGG
                     SVWIIVLLIEFVIKKECMGGGDIKLFAMLGLYMGVKNGLLTALLSVYVGAVYGICVII
                     ISRIKGKEYNSVIPYGPFISIGALITILCGKKLLELYFSMVI"
     gene            50022..50672
                     /locus_tag="MJE33_000335"
     CDS             50022..50672
                     /locus_tag="MJE33_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBY9646315.1"
                     /translation="MEYIIIISLFFAHLINKSLCVFCDREKKNLYNTFFIYIITVVLS
                     VLIYLKFGISVECVQYMVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLAFFNI
                     AQFDYINHIFGLLIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIVLSFI
                     LAFLFFIILYITKVFKDVDIRKQIPFTPFISLSTILIIFTEYNIFNFYYNLLYKFI"
     gene            50686..51246
                     /locus_tag="MJE33_000336"
     CDS             50686..51246
                     /locus_tag="MJE33_000336"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBY9646316.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKVDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITNCLGSNDFPRVRVGVSKPEAGQDLADFVLSRFRKEESDNINE
                     ALEKAADAIDSIIRENIDMSMNKYNG"
     gene            51263..54649
                     /gene="mfd"
                     /locus_tag="MJE33_000337"
     CDS             51263..54649
                     /gene="mfd"
                     /locus_tag="MJE33_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBY9646317.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYMEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDSLDLEKLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGVENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTSNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            54680..55675
                     /locus_tag="MJE33_000338"
     CDS             54680..55675
                     /locus_tag="MJE33_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBY9646318.1"
                     /translation="MKKVITLVIAMILVVSVTACSSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFAKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSDKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDTSASDGGKLGFFSRGQMVAEFEDAAFSMKKGEVSDLVETQYGYH
                     IIKVTDRINEQTSFEDAKETIKDQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            55799..56338
                     /gene="spoVT"
                     /locus_tag="MJE33_000339"
     CDS             55799..56338
                     /gene="spoVT"
                     /locus_tag="MJE33_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HBY9646319.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            56458..58071
                     /locus_tag="MJE33_000340"
     CDS             56458..58071
                     /locus_tag="MJE33_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBY9646320.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNVMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLVFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICVGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            58178..59626
                     /gene="mazG"
                     /locus_tag="MJE33_000341"
     CDS             58178..59626
                     /gene="mazG"
                     /locus_tag="MJE33_000341"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HBY9646321.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLINSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIAVEILPSMSFVDAMFNYLAIDPSEGFKLVDAFEIENSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEHYDYNQEVCIVNGAGVKDLESKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     DFIEKSAMNLNKKLEDMTLEEMDEFWNQAKKQ"
     gene            59715..59993
                     /locus_tag="MJE33_000342"
     CDS             59715..59993
                     /locus_tag="MJE33_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBY9646322.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            60065..60307
                     /locus_tag="MJE33_000343"
     CDS             60065..60307
                     /locus_tag="MJE33_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBY9646323.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYEILE"
     gene            60369..60623
                     /locus_tag="MJE33_000344"
     CDS             60369..60623
                     /locus_tag="MJE33_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HBY9646324.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            60624..61157
                     /locus_tag="MJE33_000345"
     CDS             60624..61157
                     /locus_tag="MJE33_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646325.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNKKLKN
                     KKKSKPKKKKNKTKKRV"
     gene            61163..61447
                     /locus_tag="MJE33_000346"
     CDS             61163..61447
                     /locus_tag="MJE33_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBY9646326.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINSLKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            61560..62480
                     /locus_tag="MJE33_000347"
     CDS             61560..62480
                     /locus_tag="MJE33_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HBY9646327.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKFVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSDKCNDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQGDDTIIVIDIGGGSTEFIVGDSEGIKFNKSENVGAL
                     RMTERFLKHDLIEDEEFEAMSSFIYGEIDKTLNHIKDRGLKTLIGIGGTITSLSAMNQ
                     ELEIYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            62570..64942
                     /gene="spoIIE"
                     /locus_tag="MJE33_000348"
     CDS             62570..64942
                     /gene="spoIIE"
                     /locus_tag="MJE33_000348"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HBY9646328.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICASKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISMGQAFFSNRYVYGLLIAGVESIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAVYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGCLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNSEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            65293..67185
                     /gene="pepF"
                     /locus_tag="MJE33_000349"
     CDS             65293..67185
                     /gene="pepF"
                     /locus_tag="MJE33_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBY9646329.1"
                     /translation="MNRKLCKAIIPLFISIFCMLIISIKADQGYQKNNDRAITVMNNN
                     DTEDDAEKKQEIDYEKTCWNLQSLFKSDDQWKKELKSFDKDTKELKNYIGKVTKSPTH
                     LSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMNDSMSKSYGNYMGICSDLEL
                     EILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDDKSEDILSKVSSISSLPSQ
                     VYELFRNMDRKTNLTPAQYASELESSDRENRKKAYQDELITYNDNINTIAGLITGQVK
                     KNIFYSQERGYESSLEMYLESDNVDEKVYNNLIRTVNSNMDSLHKYIDLRKKVLKLDK
                     VHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYNDVVYKAFNEKWIDVYSHEN
                     KVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVYEYLSSKNQNYFNSNPSIF
                     THEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKDTLYTQTMYAEFEKTIHEL
                     VEKGKSVNALVLNDVWGQLLKKYYGQSYELDQLSKVGWARIPHFYNSFYVYKYATGCS
                     AGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINLTNTKPIENTIKKFDSLVA
                     ELEKLL"
     gene            67299..68264
                     /gene="galU"
                     /locus_tag="MJE33_000350"
     CDS             67299..68264
                     /gene="galU"
                     /locus_tag="MJE33_000350"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBY9646330.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEVVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEVNEARELEIQK"
     gene            68764..70368
                     /gene="rho"
                     /locus_tag="MJE33_000351"
     CDS             68764..70368
                     /gene="rho"
                     /locus_tag="MJE33_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBY9646331.1"
                     /translation="MRRIFLILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNEL
                     IEIINSKSKNEEKVNEETKEKNEDIKEDIKNRKENIERESIEKEVEYKSPTKSYNKKE
                     IDTQNISQNQEHRNQRNDYNKTTSVNDSNKSSNISNRMVRNNNKNYYMPKQVDESKIV
                     DEFNTSKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGI
                     TRHPKSGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRI
                     IDLISPIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDM
                     KESIEGDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLT
                     ISPTGRTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEF
                     KGTGNMELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVM
                     EITDKVIELIKRTKDNKEFVKSIKNL"
     gene            70521..70721
                     /gene="rpmE"
                     /locus_tag="MJE33_000352"
     CDS             70521..70721
                     /gene="rpmE"
                     /locus_tag="MJE33_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HBY9646332.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            70866..71759
                     /locus_tag="MJE33_000353"
     CDS             70866..71759
                     /locus_tag="MJE33_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HBY9646333.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFVRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            71777..72625
                     /gene="prmC"
                     /locus_tag="MJE33_000354"
     CDS             71777..72625
                     /gene="prmC"
                     /locus_tag="MJE33_000354"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBY9646334.1"
                     /translation="MTIKDIIIKYSDKLKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTKFMGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDISETALEIAKKNAIINEV
                     GEKIKYINSDLFTAISDSNIKFDIIVSNPPYIKKQDIETLHKQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKCGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            72669..73733
                     /gene="prfA"
                     /locus_tag="MJE33_000355"
     CDS             72669..73733
                     /gene="prfA"
                     /locus_tag="MJE33_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBY9646335.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            73862..74542
                     /locus_tag="MJE33_000356"
     CDS             73862..74542
                     /locus_tag="MJE33_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBY9646336.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGLSGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDVSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            74571..75611
                     /locus_tag="MJE33_000357"
     CDS             74571..75611
                     /locus_tag="MJE33_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBY9646337.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKEEVYDLAKDISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNVIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVATKINDMIKSNRDKGLKTGVMC
                     ISRNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKGVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            75628..76077
                     /locus_tag="MJE33_000358"
     CDS             75628..76077
                     /locus_tag="MJE33_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HBY9646338.1"
                     /translation="MNILIVCTGNTCRSPMAEAILRKAIKESGRSIEEYSISSAGIST
                     ANGMGASENSIEVLKEIGIDLSNHRSKVITKKLIDESDIILTMTKSHKEILVQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDIYRSTMREIMYSVNEIVKKI"
     gene            76094..76546
                     /gene="rpiB"
                     /locus_tag="MJE33_000359"
     CDS             76094..76546
                     /gene="rpiB"
                     /locus_tag="MJE33_000359"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBY9646339.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLESKGIECVDYGTNNATDSVDYP
                     VYGEIVANSVINKEVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNDA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            76582..77211
                     /gene="upp"
                     /locus_tag="MJE33_000360"
     CDS             76582..77211
                     /gene="upp"
                     /locus_tag="MJE33_000360"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBY9646340.1"
                     /translation="MSKVVETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIAEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            77512..78327
                     /locus_tag="MJE33_000361"
     CDS             77512..78327
                     /locus_tag="MJE33_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HBY9646341.1"
                     /translation="MKNCIVVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKKVTVEGVECIFCNTEKEIEEVLSQHKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPLTIALGPGFCAGEDVDVVIETMRGHNLGRIITNGRAEKNTGVPG
                     AIKGFSKERVMHSQYDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGAKVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            78553..78990
                     /locus_tag="MJE33_000362"
     CDS             78553..78990
                     /locus_tag="MJE33_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HBY9646342.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            79175..79402
                     /locus_tag="MJE33_000363"
     CDS             79175..79402
                     /locus_tag="MJE33_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HBY9646343.1"
                     /translation="MSKRNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            79407..79793
                     /locus_tag="MJE33_000364"
     CDS             79407..79793
                     /locus_tag="MJE33_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HBY9646344.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAVRLFIMAVVLIASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            79796..80500
                     /gene="atpB"
                     /locus_tag="MJE33_000365"
     CDS             79796..80500
                     /gene="atpB"
                     /locus_tag="MJE33_000365"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBY9646345.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGEILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            80569..80829
                     /gene="atpE"
                     /locus_tag="MJE33_000366"
     CDS             80569..80829
                     /gene="atpE"
                     /locus_tag="MJE33_000366"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBY9646346.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            80952..81449
                     /gene="atpF"
                     /locus_tag="MJE33_000367"
     CDS             80952..81449
                     /gene="atpF"
                     /locus_tag="MJE33_000367"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBY9646347.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKSDLAEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQERQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            81446..81991
                     /locus_tag="MJE33_000368"
     CDS             81446..81991
                     /locus_tag="MJE33_000368"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBY9646348.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIDGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            82008..83510
                     /locus_tag="MJE33_000369"
     CDS             82008..83510
                     /locus_tag="MJE33_000369"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBY9646349.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQPIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            83538..84416
                     /gene="atpG"
                     /locus_tag="MJE33_000370"
     CDS             83538..84416
                     /gene="atpG"
                     /locus_tag="MJE33_000370"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HBY9646350.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIAKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMAVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            84429..85823
                     /gene="atpD"
                     /locus_tag="MJE33_000371"
     CDS             84429..85823
                     /gene="atpD"
                     /locus_tag="MJE33_000371"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBY9646351.1"
                     /translation="MANVGKVVQIVGAVLDVKFDGEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVRKAKGSM"
     gene            85826..86086
                     /gene="atpC"
                     /locus_tag="MJE33_000372"
     CDS             85826..86086
                     /gene="atpC"
                     /locus_tag="MJE33_000372"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HBY9646352.1"
                     /translation="MASEFSLKVVTPSNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFINVTQDKTVILTESAEWI"
     gene            86548..86928
                     /locus_tag="MJE33_000373"
     CDS             86548..86928
                     /locus_tag="MJE33_000373"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBY9646353.1"
                     /translation="MNIFDIGVDIIEIDRIRKAVDKNNRFLEKIFTDREIEYFNSKNF
                     KAESIAGNFAAKEAISKSIGTGIRLFNFKDIEVLRNEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
     gene            86943..87404
                     /locus_tag="MJE33_000374"
     CDS             86943..87404
                     /locus_tag="MJE33_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBY9646354.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            87425..88015
                     /locus_tag="MJE33_000375"
     CDS             87425..88015
                     /locus_tag="MJE33_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646355.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQGDKILVKNP
                     KISDVVELPNTGKNNQYLFVGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            88030..89187
                     /gene="alr"
                     /locus_tag="MJE33_000376"
     CDS             88030..89187
                     /gene="alr"
                     /locus_tag="MJE33_000376"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBY9646356.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEESVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTG
                     KETIATVPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            89220..89507
                     /locus_tag="MJE33_000377"
     CDS             89220..89507
                     /locus_tag="MJE33_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HBY9646357.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            89476..89859
                     /locus_tag="MJE33_000378"
     CDS             89476..89859
                     /locus_tag="MJE33_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBY9646358.1"
                     /translation="MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQND
                     VGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREK
                     IGCLDENMMIKVDDGLQISLGLFTF"
     gene            90030..90857
                     /locus_tag="MJE33_000379"
     CDS             90030..90857
                     /locus_tag="MJE33_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBY9646359.1"
                     /translation="MRDHKGLNEIARIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEELNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYTLLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGADNE"
     gene            90850..91791
                     /locus_tag="MJE33_000380"
     CDS             90850..91791
                     /locus_tag="MJE33_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBY9646360.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKSF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGISAR
                     VINMSSIKPIDKDLILESAKETNAIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIIKTVKEVIIAKRM"
BASE COUNT        36978 a         9903 c        16943 g        28009 t
ORIGIN      
        1 tattaaaaac ttaatagaaa tattaagcat aaagattatg tggttacaat agcagagagg
       61 atacacctgt tcccattccg aacacagaag ttaagctctc tagcgctgat ggtacttggt
      121 gggaaactgc ctgggagagt aggacgtagc cacgtaattt tttttatttt ttgtcaaaaa
      181 aaacaaaaaa tacgtatttc ttttttcatt tcgggaaaac taaacacagg aattatggac
      241 tttataaaaa tttaattctt gaggagtgat ataagtgaaa aaagttgctt taattttagc
      301 tataataggt gcgcttaact ggggtgcaat tggtattcta ggtacagact taataggtag
      361 tatctttggt ggaacatatg aaatggtaag tagaataata tacttcattg taggtttagc
      421 tggattatac ttaataccaa gtttaatgtc tgatgataga gaataattta tgcttttatg
      481 ttaaatacct cattctaaat gtttagaatg aggtatttaa ttttataaaa atattaattt
      541 gtttcttttt taatggtaaa atagataaga attgaagttt taaaaagaag tattatgtaa
      601 aaaaggagta ctattaatat atgtttatta ttaatgaatt aaataagatt gtaaatgaag
      661 aagtaatatc ttttcatatg cctggtcata aaaagggaca ggtttataaa aagttaggat
      721 ataaagatat actaaaaaat ttgtataaaa tggatacaac tgagatatta ggaacagata
      781 atttacactt tccggaagga attataaaaa attcacaaaa aaaagcatca agagtattta
      841 aaagtgatta tacttgctat ttagtaaatg gtagtagctg tggaatacaa tctgcaataa
      901 tgtcagtatg tagtcctaaa agtaaaataa taacaaatag agactgtcat cagtctgtta
      961 taaatggatg tattttagga gatatagata tagagtatgt gccttgtgag ataagtgaag
     1021 agacaaatgt cttaaaaggg atatctataa gtaatgtgat tgatataata gataaaaact
     1081 tggatgcaaa agcagtactg cttacttatc ctacatatta tggaatgaca tatgatttag
     1141 aatacatatg taattatgca catagcaaaa agatggtagt gatagtagat gaagcacatg
     1201 gtgcgcattt agggcttagc gagaggcttc ctaaaacagc tcttgagcaa ggcgcagata
     1261 tagttgtaca gagtacacat aaaactcttc cagcatttac acaatcgtca atgatacata
     1321 tacagggaga ccgagttgat atagataaaa tattgaacat gttaagaatt acagaatctt
     1381 caagcccttc atatttatta ttatcttctc tggaattagc agtagatata tatgaaaata
     1441 aaggaaaaga attaatggaa gagcttttaa ataatattat aactttcaaa aataaagttg
     1501 attcaaatat taatatatac gacacaaatg ataaaacaaa aatatttata tcatctaaaa
     1561 atatgggttt aacaggttat gaactagaaa atattttaag aaacaaatat aacatacaag
     1621 tggagttatc taattattat ggagttcttt taatatgtac aataggaaat actgttgagg
     1681 atttctctag tttagaaaca gcattaaaca atattgtaaa ggaagacttt aaatttgaaa
     1741 agttagaaaa taaaaaatac cctgttgtta ttccaaagaa aatattaaca cctagagaag
     1801 cattttataa aactaaaaaa agtgttaaaa tatatgacag cataggtaaa gtttgtggtg
     1861 aatgtatagt tccataccca cctggaataa gtgtaatatc tcctggtgaa gtaataagta
     1921 aagaaataat agactatcta atgttttgtc attctaaagg catgattgtg agtggattga
     1981 aagatgtaga tttaaatttt atacaaataa tagatttaga agacatttaa aaattatgat
     2041 ataaaagtag atagaagtag atagtttaaa attgtgaaga gaaataaata gactgcttat
     2101 atagagctat ctaataaaag tactaaaaaa gtaaaaagtg tttgtatgga ggatatgaaa
     2161 atgaaaggta agttaataat tatagagagt ggttcagatg caagtggaaa ggctactcag
     2221 acagaaatat tatatgaaag attaaaaaaa gaaggctata tgattaaaaa ggtagaatat
     2281 ccaaattatg aatctccatc atctgccttg gtgaaaatgt acctaaaagg agactttggt
     2341 aaaaatgcat cagatgtaga cccatatata gcatcaactt tttttacagc tgataggtat
     2401 gcatcattta aaaccgattg ggaggagttt tacaatgaag gtggaataat tatcgcagat
     2461 agatatacaa cctctaatat ggtacatcag gcctctaaaa tggatatttg tgatagagaa
     2521 gagtatttaa attggttagc tgattatgaa tttaatttat ttaagatacc acaacctgat
     2581 tgtgttatat ttttagatgt gcctattgaa tttagtaaaa agcttatgga gaatagaaaa
     2641 aataaaatta caggggaaga aaaaaaggat attcatgaaa gtgacataga gtatttagag
     2701 aaatcttaca acaatgcttt atatatagca gataaatatg attggaaaaa aataaattgt
     2761 gttttagata acaaattaag aagtattgaa agtatacata atgaaattta taaaatagtt
     2821 cttgattcaa taaaacctat ggagagataa atatgtattt tgaaaatatt atagggcaag
     2881 attttgcaaa gaaatattta acaaattcta taaagaaaaa taaacttaat aatgcgtata
     2941 tgtttgaagg aatggatgga ataggaaaaa agaaatttgc agatgaatta tcaaaactat
     3001 tacttgatta tgaaaatctc gaaaatagtc cagactatgt actcataaaa ccagatggaa
     3061 atagtataaa aatagctcaa atcagaaatt tacaatcaga tatagtaata agacctcata
     3121 aagactataa aatatatata atcaataatg ctgaaaaaat gactgtggaa gctcaaaatg
     3181 cacttttaaa gacgctagag gaacctccaa actatgctat aataatctta gtcacaaaca
     3241 acaaggaatc tttattagaa actataaaat ctagatgtga tataataaag ttctcaccta
     3301 taccaattga agacttaaaa cgttatttga taaatacagg tatagaagaa gaaagagctc
     3361 aattattggc tattttttcg agaggaagta ttgagaatgc tctaaatcta tctcagtctt
     3421 ctgagttttc aatgatgaga gaagatatac aacagtatat tcagataatg ttagataaaa
     3481 acatagtgga gattttaaat ataccaaata atatggaaaa atatagaggt aagattatag
     3541 ctttattaga tatgatgata aactattttc gagatatcat cctattaaaa gaaaatgtaa
     3601 acaagaacat gttaataaat gtagataagc tagttttcat tcaaaatatg agtggaaaaa
     3661 ttagttattc tcaattgtcc aagattattg atataataga agatgcaaag agtaaaataa
     3721 aaagcaattg caatttcaat ataagcatac aagttatgtc tttaaatata tacgaggtga
     3781 ttaaatgata aagatagtag gtataaggtt taaaagtgct gggaaaatat attattttga
     3841 ccctgttgat tttaatatag agcaagatat ggatgtagtt gtagaaactg caagaggctt
     3901 ggagtatggg aaagtagtcg ttggtccaaa agatatggat gaaagcgaat tagtttctcc
     3961 tttgaagcca attataagaa tagctacaga agaagataaa aaagtatatc tagaaaataa
     4021 ggaaaaggca aaagaaactt ttgagttgtg ccaacaaaaa ataaaagaac atgagttaac
     4081 aatgttttta atagattgtg agtatacatt tgataggaat aagttgattt tttactttac
     4141 agcagaagga agaatagatt ttagagaatt ggtaaaagac ttagctgcta tatttaaaac
     4201 tagaatagaa cttagacaaa taggtgtcag agatgaagct aaatctatag gaggtctagg
     4261 gccttgtggt agaaaacttt gttgttcatc ttggttaggt gattttcaac cagtttcaat
     4321 aaaaatggca aaagaccaaa gtctatctct aaatccaaca aaaatatctg gaatatgtgg
     4381 gagattattc tgttgcttaa aatatgagca tgatgtatat agtgaagcaa tagaaaaaat
     4441 gcctgttgta ggtgcattgg tgcaatcaga tgatggaaaa ggaaaagttg tagaagtgaa
     4501 tcctttatta gaacaaatta aagtggaatt tcaagacaag acagtaaaga tatacacaag
     4561 agaagatata aaaatactta gagagcctaa aaaatgtgat ggctgtggtg gtaaatgtaa
     4621 ggataatgat ggattagatg aagccacact aagagagcta aagaaattag aagattaaat
     4681 ttaaggtagg gcttttccta ccttttttaa agcaattaat gcttaaagtt attaatattt
     4741 gaggagaatt tatatatgca attaaaagat tcagaaagaa tagatgattt acaattaaaa
     4801 ggtcttaaaa ttatacaaga tactaatggt ttttgttttg gtatagatgc agttttatta
     4861 gccaatttta ctaaaataaa aaaagatgcc aaagtagtag atttgggtac tggaactgga
     4921 attataccta ttttgatagc tggaaaaagt gaagctaaaa aaattattgg cgtggaaata
     4981 caagaagatg tatatgaaat ggcaactcgt tctataaaat taaatggatt agaagaaaga
     5041 gttgagatta taaatgaaga tataaaatac atagacaaag tcttagacat aaatggttac
     5101 catgtagtca catcaaatcc tccatatatg catattgatg gaataaaaaa tccaaatgat
     5161 aaaaaagcaa tatcaagaca tgaagtaagg tgcaatttgg aagatgtaat tagagctgca
     5221 tccagattgg ttatgcctag aggaaaattc ttcatgatac atagaccaac aagattggtt
     5281 gatataataa ctttagggag aaaatataaa ttagaaccaa aggttattca atttgtgcat
     5341 ccaagacctc aaaaagcacc gaacttagtt ttggttcaat ttgtaaaaga tggaaggcca
     5401 gagcttaaaa tattggaccc attgtatgtt tatggagaag atgggaatta tacaaaagaa
     5461 ttaaaagcaa tatataataa taaagatata ggagagttgt aaatgagcgg gaaattgtac
     5521 atatgtccaa caccaatagg aaacctagaa gatataacat atagaacttt aagaacatta
     5581 aaagaagtag acttgattgc agcagaagat acaagacata gtataaaact tttaaatcat
     5641 tttgaaatct caaaaccact aactagttat catgaacata ataaggattc aaagggagat
     5701 tatttgataa ataaactaat tgatggtgaa aatatagctc tgataagtga tgcaggtatg
     5761 cctggtatat ctgacccagg agaagagata ataaaacaag ctatacaaaa taacatagag
     5821 atagaagtat tgccaggtgc aacagccttt gtaacggctt tagttggctc tggtatggac
     5881 acacatagat ttgtttttga gggcttttta gatagagata aaaaagttag gaaagcacaa
     5941 ttggaagaat taaaggaaga aagtagaact ataattttct atgaatcacc acatagattg
     6001 aaggatacac taaaagatat gcttaaaatt cttggtaata ggaatatttc tataaataga
     6061 gaacttacaa aaaaatatca agaaataata agagaaaata tagaaactac cataaaaata
     6121 tttgaggaaa aagaagtaag aggagaattt gtattaatag tagagggttt tcatggtgag
     6181 aagtcaaaga aagaaaatta tgatgggctt acagataggg aatatgtttt aaaactaatt
     6241 gaaagtggta tgaacaaaaa agatgcaata aaaatagttt gtaaagatag aaagttaaag
     6301 aaagatgtgg tttataaaca agttttagat ttataaaacc tgaatctgag gtgattttat
     6361 ggaaataggg aaaattataa tagagagttt aaagagggaa gatacacata aaatatttat
     6421 agaattagat aataagcatg aattagaaaa aataattcct aaaataagct gtatgaagtc
     6481 tgtaggtgaa tgtaaatatc atgtagtaaa ttgttttgaa cattctataa acgcattaaa
     6541 agaattagaa atagtattaa atgataagga tttttttcca atacatctaa gaaaacatgt
     6601 gtcaaactat ttaaacacat atattgaaga aggaataaat aagttacatg tattaaagct
     6661 gggaacattt ttacatgata taggtaagcc tgacagtatg accttagatg aaaccggaag
     6721 agttcatttc acaaatcatg aaaaaatagg tgctcaaata attgacaata tgggaataaa
     6781 gttagagtta tcaacagaga catatagact tataagtaaa tatgtaagat atcatatgat
     6841 acttttgtct ctatataaga aaaatgattt gagtaggaaa gaattaatta atgtatttaa
     6901 cttagtagat gaggatacta taggtgtaat aatgcttggg tatgcagata tagtgtctac
     6961 agtaaaatta ttgggcaaga gtggagaagt cagtgtgtta aagacatata tggaatatat
     7021 tttaacaaat tacttgtata agagtaatta tacacatgta taaatttagt taatataatt
     7081 gaattttaga tgattaattg aagtttgaaa tatacggatt tcaaaaacat aaaagaagtt
     7141 gtctttgttg taaaattatg aaagacaact ttttttatat caaaaattga aattttatta
     7201 aggatactta atggtctatt aattaataaa ccattagtat ttaatttata ttacaatatt
     7261 gattttgctt gtattggcgt tgatagtata acagaggtct tagtagaacc aattttcttt
     7321 atagtatcaa tcacattttc tagctcgtac atatctttta caattacttt taaaagtaag
     7381 caatcatcac ctgttatatg gtgacattct acaatcctag ggtcctttgc agctgactca
     7441 ataaattctg tatatccatt gcttggaaga gaaatatgaa taaatgcctt tataactctg
     7501 cctaatgaat ctgggttgac aatagcttta tatccttcta taacaccaga ctcttctaat
     7561 cttttgactc tttctgaaac tgcaggagaa gttaaaccaa ctatttttcc taagtccttc
     7621 atagaaattc tcccatcatc ctgtaaaatt tctatgattc tgtaatctgt aacatccata
     7681 aataaacagt cccccttaaa taaaaataat taatgctata aaactttttt agaatctatc
     7741 atttgtgttt ttaaatacca aagatttcta gataaatcaa ccttataaat gtgtggtttt
     7801 gaaagtcttt tgtattgttc ccagatagta cttaattcat attgtacatg attttttatt
     7861 tcaagaacag cttttttatt atatttgcat ctgcctttta tatacaatgg tttaagaagc
     7921 tctttaactt tgtaatttgt aaataccttc gttttccatg tataagtagg atggaagatt
     7981 tctaaaggtt tagactcatc tataacttca tcatgtaaca taatcaaatc tgcctctgcc
     8041 ttattgtctt cgttatatat tcttataact ttcttgtaac caggattatt tattttttct
     8101 ggattctcag aaatctttat cttaggttca agaactcctt tttcacaaga tgcagctaat
     8161 ttataaactc ctccaagaga aggtgaatcg aaagatgtaa tgagctttgt acctactccc
     8221 catgaattta tagtagctcc ttctgcttta agtgatgtta tggtatattc gtctaggtca
     8281 ttagatgcag tgatgctgat atctgaaaag ccagcattat ctaattgttt tttagcttct
     8341 ttagataaat aagctaaatc tccagagtca agtcttatac ccataggttt gtatcctttt
     8401 tcagaaatat ttttgaatac ttttattgca ttaggaactc cactgtttaa tacatcataa
     8461 gtatctacaa gtagtaggca tttatctgga taaacatcag catatgcttg gaatgcttca
     8521 agttcattat caaatttttg tacccaactg tgagcttgtg taccaattat aggtatatca
     8581 aacattttcc ctgctagtac gtttgcagtt ccagcacaac caccaactac agcagcccta
     8641 gccccatata tccctgcatc tggaccttgt gcacgacgta atccaaattc aaatacaggg
     8701 tctccttgag ctgcaaagca aactctagag gctttagtag caataagaga ctgaaaatta
     8761 actattgtta acaatgctgt ttcaataagt tgagcttgat ataaaggtgc cttaacagta
     8821 attaatggct cattagggaa catgatagtt ccttcctcaa cagcatatat atctcctgtg
     8881 aatttaaatt cttttaaaaa ttttaagaac ttgtcagaaa ataaatttaa gttttttaag
     8941 tactctaaat cttcatctga aaaatgaaga ttatctatgt attcaacaac ctgatcaata
     9001 ccacaaacta tagtataccc tccatcacaa gcgttttttc taaaaaacat atcaaataca
     9061 acaatgtctt catgaatatt cttttcaaaa tagccattta acatagtaag ctgatataaa
     9121 tcagtaagaa gtgttagatt tctcattatt tctctcctta attattatta aatattaaaa
     9181 attgtaaaaa ctcccacact tatactaaaa cataatatag tacaactttc aagtataaag
     9241 tattacctaa ctatcataaa attatttaaa gaggaggatg attataatag gaaaaatatg
     9301 gttttatatg gtatcgatag gaataatagg aagcttgatt tctaaaaata tgggtgaatt
     9361 aaataaagtt atattaagtg aaacatcaaa aggtatagag tttgcaataa gtctagctgg
     9421 tatcatggca ctctggatgg ggataatgaa tatcgcaaaa gaatcaggtt taattgaaaa
     9481 gataggtcaa aaactaaatc ctataatgag aagattattt ccttctattc caaaaggtca
     9541 taaagcaatg tcttatatag ttatgaatat tgctttaaat atgttgggtg ctggaaatgg
     9601 tgcaacagca tttggtttaa aagcaatgag tgagcttcaa actttgaata acaagaaaga
     9661 tacagccact aatgatatga ttatgtttat ggtaatcaat atatcatcta tgcaaataat
     9721 tccatttact ataatcaaac taaggatgga tatgggggct caaaatccaa gtgaaataat
     9781 ttttacgact ttatttgcaa ccataatatc tacagcagta gcaataataa catgtaaatt
     9841 ttttcaaaga agatatagat aaaggggatg tagatacatg gagatatttt caaacttact
     9901 tataccaata ataattctgt acatagtagt ttatggtaaa tataaaaaaa tagatgtgta
     9961 tgatagtttt gtaaaaggag caattgatgg gttaaaagca gcatgggata tcctaccata
    10021 tataataggt atatttttgg caataggaat attcaaaact ggtaaaggtc ttgatatgtt
    10081 agaatggcta tttacaccaa tagcaaatat gatgagtata ccaaaagaat taataggtct
    10141 aataagtgta aaacctttat ctggaagtgg tgctttaggg atgtacagtg aacttgcaaa
    10201 tagagttgga ataggaagtt tagtagaaaa aatgggtgct acgattgtag gttcttctga
    10261 gactattttt tacacaatgg caatatacta tggaagtctg aagataaaaa atactagaca
    10321 tacattatca tgtgctatga ttagccatgt tgcaggtgtc atagcagctg tttttatttg
    10381 ctatgtaata tttgtataat ttgttgacaa tatgggaaaa attttataat atagtgatta
    10441 tatagaaaca aatagtaata caaattacaa gctatgaaaa gaagagtaaa taaaggtttt
    10501 ttctatcaga gagctaggtt aggtgaaagc tagtgtcaaa agctttattg aagatggtct
    10561 tggagctttt tatctgagca agtgtgctag ggtaaaacgg ttggacacgt tatagtctac
    10621 taaggtatct ataaaatgtt tctacatttc atgtgaaaca taactattat agatgaatta
    10681 gggtggtacc gtgaataatc tcgcccctat gttaactcat aggaggcgat tttttattat
    10741 ttaaatgaaa gaatcatatg attacgaacg ataaaaaatt aggaggtaaa taacatgagc
    10801 aaaccgagtt tttatgtaac aacaccaata tactatccaa gtggaggatt acacatagga
    10861 catacttatt caacagtagc tgcagataca atagctagat tcaagcgttt ctgtggatat
    10921 gatgtaaagt tcttaacagg aacagatgag catggtgaaa aaattcaaaa gaaagccata
    10981 gaacaaggaa tgtcagagat agagtatctt gatggaatga taaaagatat aaaggcctta
    11041 tggaatacta tggatatatc ttatgatgat tttataagaa ctacagaaaa aagacataca
    11101 gacataatac aaaagatatt tactaaacta tatgagcaag gtgatatata taaaggtgaa
    11161 tatgaaggta gatactgtac tccttgtgag agtttctgga cagaatctca attactagaa
    11221 ggaaataaat gccctgactg tggaagagaa acatatttag taaaagaaga atcttatttc
    11281 tttagattat ctaaatatga agacagatta aaagagttat ttaaagatga tagtttctgt
    11341 tttcctgctg ctagaaaaaa tgaaatggtg gcaaactttt tagacaaagg attagaagat
    11401 ttaagcgtaa ctagaacgac ttttgactgg ggtataaaag ttccttttga tgaaaaacat
    11461 gtaatttatg tgtgggtaga tgctttatgt aactatataa cagcattagg ttatatgact
    11521 gataatgatg aagaatttaa aaaatattgg cctgccaatg ttcaaatagt aggtaaagaa
    11581 atagtgagat tccatacaat aatatggcca gcgcttttaa tggctttagg tctagaagtt
    11641 ccaaaacaag tatttggaca tggatggata ctatttgctg atgataaaat gagtaaatca
    11701 aaaggtaatg tggtttatcc agaacctata atagaaagat atggaataga cacacttaag
    11761 tacttcttat taagagagtt tgcatttggg caagatggaa gttatactca tagaaatttt
    11821 gtaacgagaa taaattatga tttagcaaat gatttaggaa acttaataag tagaactgta
    11881 gctatggttg aaaaatacaa taatggtata ataccaacag caaaagtttc aactaatttt
    11941 gatgctgact taaaagaaca agctgtttca actagagaaa actttgaagc tgaaatggat
    12001 aaaatgcaat tccatgaagc attagagagt gtatggaaat tagttagaag aactaacaaa
    12061 tatatagatg aaactatgcc ttgggcacta gctaaagatg aaactaagaa agatgaatta
    12121 gacacagttt tatataattt atgtgaatct ataagaataa tcgcaacact aataaatcct
    12181 ataatgaatg agactgctaa taaaatatat gaacatatag gaataaaagg tcaagatgat
    12241 ataactacat gggaaagtac aaaaacattt ggtcttattg gagaaaatgt aaaagtattt
    12301 aaaggtgaac ctttattccc aagattagat gtagaaaaag agatagaaga attaactaaa
    12361 atgttctctg ggaaacctcc agtagaagaa aaacctttag agcataaaga agaaattact
    12421 attgatgatt tagacaaaat agagcttaga gtagggaaaa taataagctg tgaaaaacat
    12481 ccaaaagcga ataaattatt agtatcacaa gttaagattg gaccagaaac aagacaaata
    12541 gtatcaggaa tagctgaata ctataagcca gaggatttag taggaaaaga ggttacagtg
    12601 gtatgtaact taaaaccagt taaattaaga ggtgtagaat ctcaaggtat gatattagca
    12661 gcaggtgatg atggagaccc atatgtttta ccatttacac aaggggctaa agatggatgt
    12721 gaggttcgtt agtaggtaat taaatagtaa ggagattaaa gatgttattt gactcacatg
    12781 cacatttaaa tgatgaaagt tttgatgaag atagagatga acttattggt tcattaaaag
    12841 ataaaggtgt agatttggtt gtaaatccag gagcagatat agagacgtcg ataacagcaa
    12901 ttgaacttgc gaaaaaatac gactttatat attcagcagt tggggtacat ccacatgatg
    12961 tatcaaaact agatgataca gcaatagaaa ctctaagaaa acttgcaact gagaatgaaa
    13021 aagttgtagc tattggagag attggattag attattatta tgattattct ccaagagaag
    13081 aacaaaaaga gtggtttaag aagcaaattg aattagctaa tgagctaaaa cttccaataa
    13141 taatacatga tagagatgca catggagata cttttgagat aataaagaat actaaaaatc
    13201 ctgaaatagg atgtgtactt cattgttata gtgggaatgt agagttagct agagaatatg
    13261 ttaagatggg ttgttatata tcaattccag gtacggttac ttttaagaat aataaaaaaa
    13321 ctagagaagt agtaagagaa atacctctag aaagattatt catagagact gattcaccat
    13381 atatgtcacc agagccacat agaggaaaga gaaacaatcc ttctcaagta tcttttgttg
    13441 ctgataagat agcccaggaa aaagggatat cttatgaaga agtatgtaga gttacaaaag
    13501 aaaatgcaaa gaaatttttt aatataaaat agacaaatta aatcttataa tgatatatat
    13561 aataagtcat tataagtata ggatgagagt gcctcaaaat gagtttttta gtttatgagg
    13621 cactcttttt gtatgaaatc aaatatattt tcatcatttc aacaatgaaa aagaagttta
    13681 tcatattttg agatagtttg tttttattta tttaattcat attttaaaat atattttctt
    13741 aacattgtta aaaaagaatt tacaagatat tcattagaat taactcaact ctaatattca
    13801 cttaacttaa gttgtttatt atattattaa agagaagtaa acgaaaagta gattaaggag
    13861 gttgaatttt gtatacagtt gatagtacta agatattgat aaatatgaac ttagagaaac
    13921 tagaaaagat atactcaatg ataaaagata catcaagtat ttctataatt aaagaaccta
    13981 acttggctac agtaatggta agagctaatg aaagtgtaaa aaacacaaca tttaatttag
    14041 gagagatatt agttactgaa tgtagtgtaa aggtagatga aagcttgggt tacggaatag
    14101 tttcagaaaa caataataag aaagccattt atttagcagt tatagatgca gttttacata
    14161 gcaataacag taaatttgat gagttaaaga attacataaa taaaagtgta tatgaagaga
    14221 acttaaggta tgaacaagag attattgatg aatttagctt gattaataaa acgaaagttc
    14281 aattcaatgc tatggattaa ataggaggag aatcatggta aattatacac aagaaatata
    14341 tagaaagatg ctagatagtg cttcatttcc tgggaaatta aataatatag ataatataaa
    14401 tatagaaaat aatacaaaac tattgaatgg agcaattggt atagcaatca ctttattaga
    14461 ccaagaagtt accttctata tagaaaatta tactcaaaaa gatataaaaa atataagagc
    14521 acttacaatg tcaaatcaaa tagattatag aaaatcagat tatatatttt tagatattaa
    14581 ttcagaagtg gatatattac agattaaaat aggttctttt gaatatcctg atgaaagtgc
    14641 aactatagta catcaagtaa atgatttatc aacgtactct caaactaagt atataaagct
    14701 tgatttgagt ggtcctggta taaaatctaa aaatagtata tatataaatg gaattagaga
    14761 agaatttata gaaagattat ctagcataaa taaagattat ccaataggaa tagatttgat
    14821 tttagtagat aaaaaaggag aaattgcatt tataccaagg tcatcaaaat taagttggga
    14881 ggtattatag aaatggctta tgtagcagtt aaaggtggag aagaagccat attacaggct
    14941 aaaaatatac ttgagtttta tagggtaaaa ggagatagct tgcctataga tgtaaagcag
    15001 attgaggaac aaatgtctta tgctgtagac aaagtaatgt ctgaaggaag tttatacaac
    15061 aaggaactat cagcattggc tataaaacaa gctcaaggtg atatattaga agcctctttc
    15121 tatgtaagag ctttaaggtc aacaatgcct agaattacat attcagaacc cataaataca
    15181 gaagaaatga gaataaaaag aagaatatca gctactttta aagatatacc aggaggtcag
    15241 tatcttgggg caactagaga ctatgaaaat agaatattga atgtagaatt attagaagaa
    15301 gatgaaaaaa agaatagaca ttttagagat agttttataa atgaattaga aacagtagat
    15361 gaaatccctg aatttacaaa aatatctaat atatttagaa aagaaaattt attacaagaa
    15421 gaagtttgtg aaaacaaatt ggaagaagtt attgatgata ttacaagaaa tagtttaaga
    15481 tttccagttt ctaggtcagc aaagttacag gcattaacta gaggtgaaag tggagtagtg
    15541 tcaggttttg cttatgctat tgtaagaggt tttggtgatg aacatccata tataagtgaa
    15601 cttagaactg gtaatgtatt tataaaagta aaacatccca tctatgaaga tgaagaaata
    15661 ataatagggg atatgctggt tactgaatgt gaaatagttt caaaagcatc acaaaataag
    15721 actgaaaatt ctaaaactaa ttcggaagat aaaaataaga tgtctttagg atatggtctt
    15781 tgtatgggga ataatgagaa taaggctata tctatggcca tactagacaa atctttggag
    15841 ccaaatagtg aaaaactagc acaagatgaa gaatttgtat tgcttcatat agatggaata
    15901 gattcattag gatttatatc tcactttaag cttcctcact atgtagattt taaagcggat
    15961 gtagaaagaa taaaatagtt tgaggaggta agtttaggtg atatataact atggtttttt
    16021 agacgaaaac acaaaaaaag aaataagaag aaagatatta aaagcaatat caatacctgg
    16081 ttatcaagtt ccctttggtt caagagagtt gcctatagct aagggatggg gtactggagg
    16141 tttacaatta acactatcat taattggtaa aaatgatgta gtaaaagtaa ttgaccaagg
    16201 aagtgatgat tctacaaatg cttgtaatat aaggagcttt attgcaagtg taagtaatgt
    16261 agaaaccaca aaagatacat tagaagctac attgattcaa acgagacata gaataccaga
    16321 agaaaaactt aaatctaatc aaatactaat atttcaagta ccaataccag aaacccttag
    16381 aatagtagaa cctagtgaag tagaaacaag aagaatgcat tcagaagaag attatagtag
    16441 gatgtgggta tatctttatg aagatatagt tagatttaat gatatatcta tagcagttga
    16501 atatccatgt aaagtaaatg atagatattt aatgaatcca tcacctatac ctagatttga
    16561 tataaaaaaa ttaaatatgt cagacaattt attcttgttt ggtgcaggta gagaaaaaag
    16621 aatttatgct attcctccat atacaaaggt agaaccttta gagtttgagg attataagtt
    16681 tgaagaggaa aagtttgaag gaaaatactg tagtttatgt aacagtacaa atacattttt
    16741 agatgaagta tatgacagtg acactaatga aaagtactat agttgctcag atacaagtta
    16801 ttgtgaaaaa gttagattaa aaaataacag tatagatgtg acaataggag gtacttggaa
    16861 tgagtaaaat aaatgactct aaaagattag aaaatataga caagcaagta ttaataggaa
    16921 gaaatatcaa tcttatttat ggagatgggt gtgaaaagtg ttttgaaagc acaggatatg
    16981 aaagcaataa tatatgtaag tattgtggtt ctatagttgc atgtaataat gtaaatttag
    17041 aactttatga aggagaagtc cttggaattg taggtgaatc tggttcagga aaaagcactt
    17101 tattaaaaat actgtttttc caagaacatg caaatagtgg agaagtatat atatcccatt
    17161 acaataaaga cacaaatata ctagagcttt cagaccaaaa aaagagatac ataaagaatc
    17221 attttatggg tattgtgtat caaaatcccc accttggttt aaatttagac tttagtagtg
    17281 gtggtaatat agcagaaaaa ttattgatgg caaatttata taatgttgaa aaaataagag
    17341 atagaggcaa agaactttta ataaaaacta atatacctgt agaaagaata gaccataaac
    17401 caaaatactt tagtggggga atgcaacaac gagttcaaat agcaaaagct ctttcaaata
    17461 accctccaat attgctatta gatgaggtta caacaggttt agatgtttct gtgcaagcgg
    17521 aagtattgga tttaataaga gaaatccaaa gagaacttaa gatttctatg atagttgtat
    17581 cccatgactt tgatgttata aagatgcttg ctgataggac aattgtaatg aaaaatggaa
    17641 gtgtaattga gtctggtctt acagaccaaa taatggagga cccacaacac ccatatactc
    17701 agcaacttat aaattcattg ctataaatat cgtttgtaaa taagcaaaat gggggttttt
    17761 aataaaatga aagaaactat acttgaagta aataatttta gtaaagactt tgagttacat
    17821 gcattgaaca agactataac ggcatgtagt aatataagtt ttgaagtttc aaaaggtgag
    17881 tttttaggaa taataggtaa atctggagca ggtaagtcta ccatattaaa aagtatctat
    17941 aaaacatata taccaacaac tggagaaata atttttaatt cagaaatata tggtaatgta
    18001 gacctggcta aaattggaga ccgagaaatg ataaatctta gaaagaaaga aataggatat
    18061 gtatcacagt ttttaaagac acttccacga ataactgcaa ttgaactggt agtacattct
    18121 ttaattgaga gtgggtttga aaaagattgt tcgtatgata tggcaaaaga tatacttaac
    18181 caatttgaaa taaaggaaaa tttatgggat gcgtatccaa ataatttttc tggtggagaa
    18241 aaattaagat taaatttagc tcaagctatg gttaaaaagc ctagactttt gttacttgat
    18301 gaaccaactg catcacttga taatcaatct aaaatatatg taaaagaaaa aatgttggag
    18361 cttaaaagtc aaggaactac aatgatagga atttttcatg atatagagtt tatggaaact
    18421 gttatagata agacctttac tatgacaaaa gggactataa gtgaaagtgg ggtagcctag
    18481 atgataaaac taattcataa tgcaaatatt gtattagaag atatgatttt agaaaatggt
    18541 catttaatca tagagggaga tacaataaaa aaagtatcaa atgataagat tgattacgca
    18601 tttgacttta atgagataga tgaaataata gatgcaaaaa acttatatgt aatacctggt
    18661 attatagata ttcacagtga tgccattgaa aaggaaatag agccgagacc aagtacatta
    18721 ttaccattta atatggcatt ttatgaacta gataaaaaac tacctgcaaa tggaataact
    18781 acagtttatc attctatatc tttaggggat ggagtaggag ttagaagtat tgataactct
    18841 ttaaagatga ttaaaaatat tgattcatat aaaaatatag atagtaagag cataaatcat
    18901 aaagttcatt taagatatga agtgttgtat tatgaaggtt tagaaaaggt tttagaacta
    18961 ttagatgaaa ataaaataga ctacttatca ataatggacc attctccagg acaaggtcag
    19021 tatacaaatc caacatttta taaagagtat gctactaaag tgtggggagt tacagaaaat
    19081 tatgtagata catggttaga tgatttagta aatctgcatg ataatttaga ttggaataaa
    19141 atagctaata ttataggtat agccaaaact aaaaatataa atgttgcatc acatgatgat
    19201 gatacattag aaaaaatgga tttcataaaa aatataggca taaatgtatc tgaatttcct
    19261 ataactcttg aagttgcaaa acattcaaag aaattgggtg ttctaacttg tttaggtgca
    19321 ccgaatattg taagaggtaa atctcataat aacaatttaa aagcaatgga tgctatattg
    19381 gaagattgtt gtgacattgt gtgttcggat tatcttccat cagctatgat aaagtctatg
    19441 tgtatagttg cagaaagaat aaataattta aataaagcag tcagtttatt tacctcaaat
    19501 cctgctaaag cagttggtat atatgatgaa agaggtagta taaaagaaaa taaaaaagca
    19561 gatttagttt tagtagatat tgatagtgaa tatccaaagg ttgtaaatac tattgtaaat
    19621 ggaaaaacag tttataaaag ggaggtataa agattgctaa cagagttaca ttgtcataca
    19681 agtatatcag ataataattt tactactgaa gaaataataa aaaaagcagc agaaaagaat
    19741 ataaatatct tagcaataac aaatcatgac acattatctg gattggatga agctcttgtg
    19801 cttggagagc attatggaat aacaataata ccaggtatag aaatttcagc ctatgattat
    19861 aaaaatagaa aaagagttca tattcttgga tataacatag atttaaacag tcaagaaata
    19921 aaaaatttgt gcaatccaat ggtatgtgac agacataaag catctgttaa gatggtaaat
    19981 aaaattatag atttaggata caaaatatct ctggaagatg taaagaaata ttcatcaaag
    20041 actggaatat ttaaacaaca tataatgaaa gctcttatag ataaaggata tacagataat
    20101 atatattcaa atctatataa agaattgttt catagaggaa acggcaaggt atatgtatct
    20161 ttaaaatatg tggattacag agatgcaata aaagctataa aaaattctgg tggaatttgt
    20221 gtgttagctc acccgggtca aatggataat tttagcgcta ttgaagagat ggtaaaagta
    20281 ggattagatg gaatagaagt atatcatcca tctcacaaca aagaactaga aaaagagtcg
    20341 ctttcttatg ctaagaagta taatctagtg gtcacaggag gttcagatta ccacggattt
    20401 tattctcaac ataattatga acttggaagc aagagcttaa ataaagaaga tttatttaag
    20461 tttaggttag ctttaggagg ggtattataa atgtcttttt tacaagtaaa aaatgtaggt
    20521 aaaagttatg gacaagttaa ggtgttaaaa gacatatcta ttgatattga aaaaggagag
    20581 tttatttgtt tactaggtcc aagtggttgt ggaaagagta cactacttag aataattgca
    20641 gggcttgagg ataaacatgg tggaaaaata ataataaatg ataaggatat gactaattca
    20701 ccacctgaaa gtagaaattt tggtatagtt tttcaatcat atgcattatt tccaaatatg
    20761 aatgtttaca aaaacatagc ctttggacta gaaaataaaa acataagtaa atctaatatt
    20821 gataaaaaag ttaaagaagt tctagaagtt gtagaattaa gtggatatga gaaaaaatat
    20881 ccatcacaat tatcaggtgg acaacaacaa agggttgcat tagctcgtgc gattgcttta
    20941 gagccagact ttttacttct tgatgaacct ttatctgcat tagatgcaaa agtaagactt
    21001 aaacttaggg agcagattag aagtttacat agaaaactag gcattacaac tataatggta
    21061 acgcatgacc aagaagaagc tctttgttta gctgataaga tggttgttat gaatagagga
    21121 gaaataatac aagttggaac gccaaaggag gtatataaaa atccagaaac accttttgta
    21181 gctgatttta taggaacaat aaattttata gatgatggta ttaataagat agctattaga
    21241 ccagaagata taaaagtaga gtctaataga gattctaaag ataaggatat aaaagtggga
    21301 gaaattttag atatcgaatt tagaggattt aactatagaa ttacagttga gtatcgttct
    21361 aagcaaatga aattagatgt agtttccaaa gttgcagaac aaatgaaact gtgcataggt
    21421 tctaaaataa actttaagat acctaaagaa ggaattgtac aatataaatc agagggatgt
    21481 gcttaatagc taggggtgaa taaatgataa catcaaaaaa taaaagaaga ataagtgata
    21541 gcacaataca aaaatatttc ttgatttttt cggtaattgt actaatagta tttatattat
    21601 ttccattagt atcattatta aaaaatgcat ttatggatac aagtggaaat tttataggaa
    21661 tttcaaactt tagtaaatat gttgaaaatc caagtcttat agcatcattt aaaaattcaa
    21721 tttttgtatc tacagtctca tctttaatat cactatgttt agcatttata tattcatacg
    21781 caataacaag aacaaacata aagtttaaag aattttttaa aactatggga atgtttccac
    21841 tttttgcacc aactatgtta tatggtattt cacttatata cctttttgga aataagggaa
    21901 tctttacaac catgggtctg gaagtaccac tatatggtcc attaggaata attatttctc
    21961 agattctatt tacatttcca caagcttttt taatattatc tgtagcactt tcgatggctg
    22021 attacagact ttatgaagca gcagatagct tgggtgctag tgaatttaag aaattttgca
    22081 aaataacaat tcctggtata aagtactcat tacttagttc attctttgta tcctttatac
    22141 ttgcatttac agattttgga gcagctaagg tagttggagg aaactacaat gtactggcta
    22201 ctgacatata taaacaagta gttggacaat ttaacatacc aatgggagct actgtttcta
    22261 tggtaatgtt aattcctgta ctaatagcat ttacattaga taagatggca agtaagaaac
    22321 aaggtatgac cataactgca aagtctatgc catataagat taaggaaaat aagtttagag
    22381 atagagtatt tttaatagta tgtagtttta taacattgct aatttttatt ttaatattcg
    22441 tttcagttat aggttcagtt ataaagttat ggccatataa tttaagcttt acaatggaac
    22501 actatacatt taaaggtatt ttgggaagtg gaataaaaac atatttaagc agtttgaaaa
    22561 tatcaacttt aacagcaata tttggaacta tatttgtatt ttttagtgct tatatgatag
    22621 aaaaggtgga taagtttcct aaattaagac aaacagctta ccttttatca atgataccaa
    22681 tggccttacc tggcttagtt ttaggtatat catatataac tttttttaac tcagcaagta
    22741 atccattaaa ttttttatat ggaagtacag caatattagt tatagtaaat gtagttcatt
    22801 tttattcagt ttgttttata acctcaaatt catctttaaa aatgttggat aaagagtatg
    22861 aattagttgc aaaatccata aatattccat tctataaagt attttttaat ataacagttc
    22921 caatgtctat aacatcaata cttgaaatag tagtatatta ttttgtaaat tcaatggtta
    22981 ctgtttcagc attaatattt ttatatacac ctcaaactca gactgcatca atatcaatac
    23041 taaagttaga tgagatagga tatataggac catcagctgc tatggcagta ttagtgcttt
    23101 taacaaatat aatagtaaga cttttgtacg aatttgtaac aaagaaatta aaaaataaca
    23161 cacaaaaatg gcagcaaaaa gatgttgcat aggggaggta aaaatgaaat ttaagaagct
    23221 agtttcttta ggagtattat caacactttt attaagtgta ataacaggat gtagtactga
    23281 ttcatcaggt tcagattcaa agtctgatag taagacaaaa ggggatttaa cagtttacac
    23341 agcaattgaa gaagatagta tagagccata tttagctaca tttaaagaga aatatccaga
    23401 tattaaatta aatattgtta gggcatcaac aggagatata acagcaagat tattagcaga
    23461 aaaggataat cctcaagcag atgttgtatg gggagttgca gccacaagct tattggttgc
    23521 agatgaccag gggatgttag aaccatatgc tccaaaaggt tctgaagaaa tagagtctgc
    23581 atttaaagat gataaagaag ttccttcttg ggttgggata gatgcttgga tgacaggtat
    23641 aacagtaaac actaaagaat taagtgataa gaacatccca gttcctagtt cttacgaaga
    23701 tttaataaag ccagaatata aaggtcttat atctatgcct aatccatctt cttctggtac
    23761 agggtactta acagtatcag cccttataca aattatgggg gaagaaaaag catggcagta
    23821 tatggataag ttacatgaga atattggtgt atacactcaa tctggttcag ctccagctac
    23881 atcagcagct tcaggagaat atcctattgg tatatctttt ggatatagag gtataaagtt
    23941 aaaagaggaa ggctatccag tagaagtagt atttccaaaa gaaggttctg gatgggatat
    24001 agaagcaaat gctcttgtga aaaaagataa tataaaagaa gcatcaaaag tatttttaga
    24061 ttgggctata agtaaagatg caatgaacga atactctaaa aactatgcag ttactactat
    24121 ttctacagga aatccaatac cagaaggatt ccctaaaaag ccattagagc aaatgataga
    24181 taatgattta aaatcagcag caaaaaatag agaagatata ctaaataaat ggatatcaaa
    24241 atatgatggt aagacagaaa aagagagtta agtaaaacta tacaaatgga gtaaaaagaa
    24301 aaattttttt taagtagatt ttattattat aatataagtt gagaataata taggtcaaga
    24361 aaagtgaata ttctataaaa atgtatagag taagtatacc attcagtttg aatggtatat
    24421 tttttatttt tagagattaa tttagaaata gatatactac agtttaaaat agtatatttt
    24481 aatttcatta ttaaagtata gatgttatat ataaagtaaa taagatatta aataattata
    24541 aactaaagta aataaaatat tgagcgatta ttaaaaagat atatatataa tttaatttga
    24601 gcagtttgtt atatattata aaaaaagaga aaaataaaag gtaaattgtt tctggataac
    24661 ttaaaattgg aagtaagaat atttaactag agaagaatta tttaagttta ggttagctct
    24721 aggagtagct gattttatag gaacaataga ttttatggat gatgatataa acatggtagc
    24781 aattagacca gaagatataa gggtagaatt taatagagat ttcaaagaaa ttatagacct
    24841 caaactcaga ccgtatcgat ataaaaacta aagttagatg atatagtgtt gtagatagga
    24901 ccatcagctg ttatggcagt attagtgctt ttaacaaata taatagtaaa acttttgtat
    24961 gaattgataa taaaaaaatt aaaaaccaag acataaaaat ggcaacaaaa agaagttgaa
    25021 taagggaggt aaaaatgaaa tttaagaagt tagtctcttt gggaatatta tcaacacttt
    25081 tattgagtgt aataactgga tgtagtaatg tttcatcagg ttcaaactta aagtctaata
    25141 ataagacaaa aggagagtta acagtttata cagcaattga ggaagatagt atagatacat
    25201 acttagctac atttaaagag aaatatccag atattaaatt gaatgtactt agattatcag
    25261 taggaggtat aacagcaaga ttattagcag aaaaagataa tcctaaagca gatgtgttat
    25321 ggggtgttgc agctacaagt ttgataattt tagatgacaa agaaatgcta gagccatacg
    25381 ctccgaaagg ctttgataag gtaaattctg catttaaaga tgataagaaa gttccttctt
    25441 gggttggaat agatgcttgg atgactggta taacagtaaa tactaaagaa ttaaaagcta
    25501 aaaatatatc agctcctagt tcttatgaag atttaataaa gccagaatat aaagatctta
    25561 tatctatgcc tagtccagct tcttcaggaa caggatattt aacagtatca gaactcatac
    25621 aaattatggg tgaagaaaaa gcatggcagt atatggataa attgcataaa aatattggtg
    25681 tatacactca gtctggttca gctccagcta tattagcagc ttcaggagaa tatcccattg
    25741 gtatatcttt tggaaataga ggtataaagt taaaaggtga aggttatcca gtagaagtag
    25801 tatttccaaa agaaggttct ggatgggaga tagaatcaaa tgcacttgtg aaaaaagata
    25861 atataaaaga agcatcaaaa gtatttttag attgggctat aagtaaagat gcaatgaatg
    25921 agtattctaa gaattttgca gttactacaa ttgacacggg aaatccggtg ccagaaggat
    25981 ttccaaagaa tccattagaa cagatgatag ataatgattt aaaatcagca gcaagaaata
    26041 gagaggctat actaaataaa tggatagaaa aatatgatgg aaagacagaa aaagagagct
    26101 aagccttgca ataaaattta gtacaaaaag cgcctagaat ataagtttta tatctaggct
    26161 cttttgatat atttatttaa aatgcatatt tctatataaa aataaatagt ttagtacctt
    26221 gattaaatta aataaaaacc ttaaaataaa gagatgtaaa gaaaaactaa aaacgaataa
    26281 aaggtgaatt tatatgataa aagaaataat agtagtagag ggtagagatg acgttacagc
    26341 agttaagaga gcaatagatg cagaacttat aactacaggt ggatttggat ttccaaaagg
    26401 tgtaatggag cgtataaaag ctgctaatga aagacgtggt gttataatat ttacagaccc
    26461 agattttgca ggtgaaaaaa taagaaaaaa aatagcatca gaagttccag gatgtaaaca
    26521 tgcatttcta ccgagagagg aagctaaaaa ggatggagat ataggtattg agaatgctac
    26581 tcctcaaagt ataatagcag ctctgaataa agttagaact gaaaatacag aaaaaagaga
    26641 tgaatttaag caagttgact taattagaaa tggtcttata ggtaatgaag aagcatcact
    26701 tagaagagat gcacttggaa aaatacttgg aattggatat ggaaatgcaa aacaattttt
    26761 aaatagattg aataattatg gagttgaaag agaagaattt atagatgcat taaaacaaat
    26821 ctaaacgtaa tgtttaatgt atgaaaaata tgtatataaa ataaattgtg gaattacggt
    26881 taatttaaga tgaattattt ataatataca ccttatttta aatcaatgta aatacatata
    26941 aataagtata attaataaaa ttaaatatca aaataagtta aaaaggagta aataatggat
    27001 agactatctt cacataatgc aacaaaggaa gtagtacaaa aatataattt taaattttca
    27061 aagtctttag gtcaaaattt cttgatagat agtaatataa ttgataaaat cttatctgga
    27121 gctagaatca caagaggaga taatatcata gaagtaggac ctggtatagg aactcttact
    27181 cgtgagatgg gtaaaatagc agaaaaagta gtggctatag aaatcgatag aaatttaatt
    27241 cctatattga aagatacctt gtcagattta gataatacag aggttgtaaa tcaagatata
    27301 ctaaaagtag acatacagga attagtaaaa gataaattaa atggtgggcc agttaagctt
    27361 gtagcaaatc ttccatatta tataactact ccaatagtta tgaaattttt agaggaagac
    27421 atacctgtaa cagacatagt agtcatggtt caaaaagaag ttgcagatag aatgaatgct
    27481 attccgggta ctaaagatta tggggcatta tctatagctg ttcaatacta ttgtgataca
    27541 gagattgtgg caaaagctcc aagacatatg tttataccac aaccaaatgt agattccaca
    27601 gttataggtc tacatgtaag agataaaaga aaatatgatg ttcataatga agacatattt
    27661 tttaaaacag taaaagcatc ctttggacaa agaagaaaaa ctcttcttaa ttcgttggga
    27721 ggtcttggtt ttcttaataa agatgaaata agagaagtat taaaagaagc taatatagac
    27781 gaaaaaagaa ggggtgaaac tcttagtata gaagagtttt cagttctttc aaatataata
    27841 aatacaaaag tatcttctaa atagaaggta ctttttttaa ttttgttaac atatattgtt
    27901 tagagaaact gtgttaaggg gggattgcag tgggttctaa aagaaagttt aaaagagact
    27961 atataatact agaagctaaa gacataaact ttagatacaa agaacgagta ctgccaaaag
    28021 cttttgcaaa agttgaagta aatgatgaaa aagcaattat tgcattatat gtagaaaatc
    28081 taaaatatgt taaagatggg tataaagcag tagcaataaa agatgaccat ggattaatag
    28141 acttaggaaa tataatttta aatgaacaag gaagaggtga atttgtttta gatttagaag
    28201 attatgatga tgagataaaa ggtattgcat tattgtatga aagaaatata cctttaattg
    28261 gatttaaagg aaataagctt gaaaattatg aggacatatt atttcaagag gtagaggaag
    28321 aagaatatga agaatatgaa gagttggaag aagaagacga agagatagag gagaatgagg
    28381 aaagtgatag agacgaagat agtgaagaag aatatattat agaagaatat gatgaagatg
    28441 agtatgaaga ctattgggaa gatgatgaag atattgaaga aggagagtta aaagcaagaa
    28501 cggaagtaga agattctgat gatgaggaaa tagaagagtt caataattat aaggaaatag
    28561 aaaattataa tagggttaaa gtacaggaag aatatataga catagaatat gatgaagaag
    28621 aagaggaaga agaagttttt gaagtaaata gagaagaaga taggtttgtt tcctcaaaac
    28681 ctactaaatc aaagaaagtt caaaaagatc agaataatat tgaaaaaata aaaagagcta
    28741 atggagtaag tcaaggagag agtggaaata aacaaaccag tacattactt atgccaagac
    28801 aaataaaaaa aggtttgaaa ttatttaaag aagtaaaacc ttttgtaaca gattatatag
    28861 ataagacaag atggtggaaa atagagataa atcctacaac actgtgtgga tatacaatgc
    28921 cttatttagg atatttaaac gtattgaatt acactatgta tagtgatgca gttttacatt
    28981 catataaata cagacactat ttatttggag ttcagtatga tgaatataat aagagaaaac
    29041 attatattta tgctatccca ggaaatagaa atgaacagcc agacaatgga agcacaggat
    29101 ttttaagata tcaagcttgt gataatagaa caaatagttt aggatattgg ctatgtttta
    29161 tagattctag acaaagaaat atagttaggt aaaaataata aaaatgaaaa aatttagact
    29221 caattcttat tttactgaaa aaattaatga aatgtagtac aaaactttaa aaactattga
    29281 taatatttaa atattacact acaatatatc ttgaaatacc tattatatta tggaggaaaa
    29341 ttatgataaa cgagcaaaga attttaaatg agtttcttga attagtacag atagatagtt
    29401 tatctttaaa agaggggaat gttgctaaag tattagttaa aaaattagaa gaaataggtt
    29461 gttcagtagt aatagacaat gctggtgaaa aagcaaatgg agaaactggt aatataatag
    29521 ctacattaaa aggaaataaa gaaggaaaga aaatattatt tagttcacat atggatacag
    29581 taactccagg tataggggta aaacctatag tggatgaagc aaatggaata ataaaaagtg
    29641 atggtactac aatattaggt tctgatgata aagcaggtat agcagctata ttagaaggct
    29701 taagatatat aaatgaaaat aacatagaac atactgatat acaagttgta ttctctatat
    29761 gtgaggaatg tggattagtt ggagctaaaa atttagacta tggaaaaata gattctgaat
    29821 atgcatttat attagatagt ggtggttctc caggtgaaat aatagtaaaa gctcctgcac
    29881 aagatgtaat aaatgttaag atattaggaa agactgctca tgcaggtctt gaacctgaag
    29941 ctggaataag tgctataatg gtagctgcca gagcaataga aaatatgaat ttacttagaa
    30001 tagacgaaga aacaactgct aacataggaa ttataaacgg tggaacagct acaaatatag
    30061 taacaggaga agttaatata gttgcagaag ccagaagtct aaaagaaaat aaattagatg
    30121 ttcaaactaa acacatggta gagacttttg aaaaagctgc gaaagatttt ggagctcaaa
    30181 ttgagataga tgttaataga gcatacacac caatagatgt tagtgaggat tctgaaataa
    30241 taaaacttgc aaagaaggct ttttcaaact taggtataga aggtcatact gaatctacag
    30301 gaggaggtag tgatacaaat atattaagta agaatggtat cgaagctata actttaggaa
    30361 ttggtatgaa aaatgctcat acactaagtg aacatatagc tataaaagac ttatatgatt
    30421 ctgctaaaat ggtagtggag ataataaagg aagcataaat ttaaataata gtgtataata
    30481 tatatatcgc ctagcttaag ctaggtgatt tttcatttta tagaagattg ataatctcta
    30541 ttgaaagcaa agagtataca tttgcatata agagagtgtt taatttatgt tcaaatgtag
    30601 gttcttaata atatttagat tgtttcataa gggaggaata aatatggaaa caaggtatga
    30661 agaagcaaat aaaataacaa tacaatcaat actttggaat atagtattaa caataattaa
    30721 ggttatagct ggtgttattg gtaattctag cgctatgatt gcggatggat tacattcagc
    30781 atcagatatt ataagttcta taggagtact aataggcaat tatgtatctt ctagacctgg
    30841 agatagagag cataattatg gtcatgagaa agcagagaca ttagtatcat ttgtgttatc
    30901 aatattgctt attttcgttt ctataacaat aggtatagag gcaattaaat ctttatttaa
    30961 cttagatgca ttaagtgttc catcaatatt accattagta gtatcagtta tttcaatcct
    31021 gataaaagag tatcaatata gaataacaat aaaagttgct aaaaagataa attcaccagc
    31081 cctaaaagct gatgcttggc atcacaggtc agatgcttta tcttcagtag ctgcttttat
    31141 aggtataggt ggttctattt tagggtttaa accattagac ccaattgcgt cagttgtagt
    31201 ggctatattt gtagctaaag taggaattag tatacttata agttctgtta atgaattgat
    31261 ggatgtttca gttgatgaag aagagataaa agaattaaaa tttattgtag cagatacaga
    31321 aggtgttaaa aatcttggtg atataaagac tagaaaacat ggagctatgg catatgtaga
    31381 tttgactata tgtgtggatg aaaatttgac agttaagcaa ggtcatgata ttgctaccaa
    31441 gcttgaaaaa catataataa aacatatgga atttgtaaaa ggtataacag ttcacgttga
    31501 accatgtact aattgccaag gaaataagtg taataattaa ttaaaactta aaaaaaagtg
    31561 taaaaagtta tataattgtt ttatgattat aaactctagg ggtggttaaa tgagcaagaa
    31621 aagaaaacta aagaaaaaag ctgcaatatt aataggagta gttgttttct ttataatata
    31681 ctttatatcc tttagaatag gaataaatat ggaaaaggat aaaagtgaag tgccaaagaa
    31741 ggtagaaaaa gaagaagaag ataataggtc tttaaggact caaataaaag agaagaagaa
    31801 ggtttatata tcagataaaa atatagaaaa cataaaaatt gaagaagata tgtgggatga
    31861 aataaagtta tttttctcag attttaagaa agtaagaaat actacttctt cttacgaagc
    31921 agtttatgaa ggttattctg atgatggagt taaattttct actgacctta acttttttag
    31981 agtatatact gtaaataaag aagaatatta taaagtacca gttagttcta aggatgcttt
    32041 taagaaactt ttagatgaga gtatatatac ttcgtttgac cttttaagtc attataaaga
    32101 ttggaaagag gttactataa ctcatggaaa agaaattaaa aaagttcata gatggaaatt
    32161 tgatgattta gcacataaga tgatttcaaa gagggttgta ggaaaagttc aacctgagaa
    32221 aagtaaggaa agaagtgact ataattttac tataaatata aaaggtgata attttgatgt
    32281 aaaagttgat acaatgggaa aagattatgt aaaagttact tctaagaaaa atgtatctta
    32341 ctatgaggtt catacaagtt tatttgatta ccttaaaaat gagatatttg aaattggaaa
    32401 gaaataagac tttaagaagg ctatcaaatt agatatttga tagcctaaat tattttatat
    32461 attatttttc aaccaacaag tctgcaacct tataaattgg accagcacca gcagttatta
    32521 ctaaatcatt atccttaaca ttttcacgta agtattttac tatatcttca aattctttta
    32581 tatagatggc atctacattg ttttgatata atttctcaac caagtcctta gaatgtatat
    32641 ctcctgggtc tttttcccta gcagcatata tatctgttat gataacttta tcagcagcat
    32701 agaatgcctc tgaaaattca cctaaaagag attttgttct agtataagta tgtggctgga
    32761 agatacacca taaagttgat ttttttaatt ttttggcagc agctaaagtt gcttttaact
    32821 cagttggatg atgtgcataa tcatctacta ctaaagcatt cttatagtac cctttagttt
    32881 caaatcttct tccgacacct ttataaattt ttatattttt tcttatagtc tctaagtcaa
    32941 taccagaaac aagagctgcc attatggcag aagaagcatt gtatatgtta tgaagaccat
    33001 atacagaaag ttcaaattct cctaaatctc ttccttcata ttctattcta aatattccat
    33061 gaccactatt gtcaaagtga atatctttta ttacagcatc attgtcagaa tctctgccgt
    33121 attttataat agtagcttta acatcataca atatgtcatc aatattttca tcatcgccat
    33181 ttattatgaa atagccttct gggggtaata attttccaaa tttgttaaaa gaagccttga
    33241 tttcatctat tccagagaaa taatcaagat ggtcttcttc aacatttaaa actatagata
    33301 tctttgggtt aaaattaagg aaactatcta catattcaca tgcttcagtt ataaagtggt
    33361 tggaattacc tatcttaaca ttaccaccta tcatacttaa gttaccacca actagtatag
    33421 ttgggtctaa gtctgcatat tcaaagattg tagataacat agatgttgta gaagtttttc
    33481 catgtgttcc agaaactgct atagaatttt tatattccct catgatttgg cctaaaaaag
    33541 ttgctctgtt cattaccaat ttattttttt ctttagcagc cacaagttct tcattgtctg
    33601 gatgaactgc tgctgtataa acaaccatat ttacatcatc tgatatatta tcttttttct
    33661 gaccaatata aatagttgcc ccttggtccc taagtttgtc taataggtat gattcgtttg
    33721 aatccgaacc tgaaacttga taacctttat tgatacatat ttcagctaat gcactcatgc
    33781 tgataccgcc aattcctata aagtgtatat tcataataaa accacctttc tttttaatta
    33841 agtcctacaa tatgaactat ttctgtggta taataatata atgttcaata ttttataatt
    33901 aatatgcata taatattata tgtattttat ttttgaatat aataaaacat aagtaatatt
    33961 ataccacaat aagaattgtt atgcattagt tagtttaacg ttatataaag gtacttgtag
    34021 gaggaaataa taatgaaatt taaaagaaca gagagaattg gagcaatagt taaaatactg
    34081 tctgataatc caaataaaat atatacttta agttatttta caaatcaatt taatgcagca
    34141 aaatcaacaa taagtgaaga tttattagtt gtaaaaaatg tatttgagaa gctacattta
    34201 ggtaaagtta taactatttc aggagcagca ggaggagtaa aatatatacc taaaacttct
    34261 atagcagaaa atgcagagtt cttaatggaa ttatgcgaga gaatatgtga caagtctaga
    34321 atactatctg gtggtttttt atacttgatt gacctaattt acgaccctac aatagctgct
    34381 aagataggaa agatattcgc atcaaatatt gagtatgtag atgcagatta tgttgtgaca
    34441 atggaaacaa aaggtatacc tatggcactt atgactgcaa aagctatgaa tttacctcta
    34501 gttataataa gaaaagatat aaaggtatca gaaggtccta cacttagcat gacatatgtg
    34561 agtggaaaca gttctaaagt tgaaagtatg agtttgccaa gaaaagctct taaaccagat
    34621 agtaaagtta taattataga tgatttcatg aggggtggag gtactataaa aggaatggta
    34681 gaccttatga atgaatttgg tgcagaggtt ataggtactg gagtattcat atctacaacg
    34741 aatccttcag aaaaaatggt aaaagattat atttcattga ttcaattgga tgttaatgga
    34801 gataaaatag tagtagaacc aaacttaaag acttttaaag atgaatatag aaatgaagat
    34861 ttagacgaag aagaagattc agaatttgaa tttgagatag atgaagatta ataaaaaaaa
    34921 tatttagaaa aatatgtaaa aataaaaaga ggatatccct agttgttcat agaattattt
    34981 aaaaaacagt ttatattaat ttattccagc gataataatt ttaatataaa ttaatttctg
    35041 tcaaaatcaa agggggatat tttggtatga agataactga cgtaagagta agaaaattaa
    35101 cagaagaagg taagatgaaa tgtatagttt caataacttt cgataatttg tttgtagtgc
    35161 atgatataaa agttatagaa ggccataatg ggctatttat agcaatgcct agcagaaaag
    35221 taggcgaagg aaatttcaga gatatagctc atccaataaa tgcagaaatg agacaagttt
    35281 tagaagatgc agttttacaa gcgtatcatg aagcattagt tcaatgggaa gtagctgctg
    35341 aataataata aatatataaa atggagagag cccgaacatt taggctcttt ttaaatgtag
    35401 aatttctatt aaaatatgta ctttttagta aaaaagtgct atattattag tgataatgta
    35461 tatgttgtat acaaattgtt agataaatgt attataatgt atattgatta agagaataat
    35521 acgaataaaa ttaaattaga ttgaatggag tgtatcaatg aactttaaag ctataatcct
    35581 tgctgctggc aaaggaacaa gaatgaagtc taaataccca aaagttatac ataaagtatg
    35641 tggaaaagaa atggttaacc atattataga tgtttcaaaa aaatcagggg taaaagatac
    35701 tgtcgtgatt ttaggacatg aagctgatgt agttaaagaa aagcttgcag aagaaattat
    35761 aatagccatg caaacagaac aacttgggac aggacatgct gtaaagatgg ctaaagaata
    35821 tattaatgat gaagatacaa tagttgtact ttgtggagat actccactta taaaagaaga
    35881 gacactaaag agactatttg aatatcatat agaaaataaa tatcatgcta cagttcttac
    35941 aacaagagtt ggaaatccaa caggttatgg tagaataata agagataaga agggtgacct
    36001 tcttaaaata gtggaacaaa aagatgcaaa ttcagaagaa aagatgataa gtgagataaa
    36061 ctctggaatt tattgtttta atggaaagag tcttagagaa gcactagatt tgctaaataa
    36121 taacaattca caaggtgaat attatttaac tgatactgct aaaataatga gagataaagg
    36181 attaaaagtt ggtgcatttg caggttcaac tattgaagaa cttatgggcg ttaattcaag
    36241 agtagaatta tcaaaggcag aagaaattat gagacgaaga ataaatgaat ctcatatggt
    36301 aaatggagta actataatag acactaactc tacctatata gaatctgatg ttatgatagg
    36361 caatgatact ataatatatc caggtgttat gttacaagga aaaacaagga taggttcaga
    36421 ttgtataatt ggaatgaatt catctataac aaattcagaa ataggtgatg gtacagaaat
    36481 aaaaaattca actataattg atagtaaggt tggagaaaat tctactgttg ggccatatgc
    36541 atacttaaga ccaaagagtg acttaggaaa taatgtaaaa ataggcgatt ttgtagaagt
    36601 aaaaaatgct attatagaag atggctcaaa agcatctcac ctttcttata taggagatgc
    36661 acatgtcggt aaaaatgtaa atattggatg tggagtagta tttgtaaact atgatggtaa
    36721 aaataaattt aaatcaatag taaaagacaa tgcgtttata ggttcaaatt ctaatttagt
    36781 tgcaccagta gttgtagaag aaaaaggata tatagcaaca ggttcaacaa taacacatga
    36841 tgtcccagat ggtgctttgg caatagcaag agaaagacaa gttatcaaag aaggatgggt
    36901 agaaaaaaag aatcaaaaag acgaccaaag taagtaatta tgtagtttat tattttggct
    36961 aaaaaattta gctcgaaatt attataaatt atattcatta gtattttaaa attttcagga
    37021 gggttacata aatgaatact agcggaagcg agattaaaat tattgcaggt aattcatcga
    37081 aagagttagc acaaaaaata gctgactata taggtgtatc agtactagat tgtgaagtag
    37141 gtacatttag tgatggtgaa atatgtgtaa acatgaatga gacagtaaga ggttgtgatg
    37201 tgtttgtagt gcaatctact aacagcccag taaatgataa cttaatggag ttattaatct
    37261 taattgatgc gttaaagaga gcatcagcag gtagaataac agcagttatt ccttactatg
    37321 gatatgcaag acaagacaga aaggctaagg caagagatcc aatcactgct aaattagttg
    37381 caaatttaat aactgctgct ggagcagata gagtattaac aatggattta catgcagctc
    37441 aaatacaagg atattttgat ataccattag accacttact aggtggaaca atattagcaa
    37501 attattttaa tgaaaagaaa atagaagatt tagtagtagt atctcctgac ttaggaagtg
    37561 ttactagatc aagaaaattt gctaatactt taaatggaga agttccaata gctataatag
    37621 ataaaagaag accgaaagct aatgtatgtg aagttatgaa cttaataggt gatgttaaag
    37681 gtaaaaatgt tatactatta gacgatatga tagatacagc aggaacaata gttaatgctg
    37741 caaatgcact taaagagttt ggagcaaaag atgtttatgc ttgttgtact catggtgtgt
    37801 tatcaggtcc agctattgaa agaattgcta attcagaaat aagtgagtta atagttcttg
    37861 atactataca acttcctgaa gagaaaagaa tagataagat aaagataaaa acagtagctc
    37921 ctttatttgg agatgctata agaatgatat tctctaatga atcagtaagt aaattattct
    37981 aatttattgt atttatgaga gttgtttaaa aggatataaa ccttttgagc aactcttttt
    38041 tgattttata cttatggaat gttatttttt tcttgttaga aaggtatata ttattgtaaa
    38101 aagagattat agtttaaaat tatgtatatt taagtaaaat aaacctaata aagggtatat
    38161 tattcaaaat attatgaata taaaaaatat tacaaaaaag tattgaaaaa attcagatta
    38221 tgtagtatta tgtaaatata aataaagaaa gaggtgtttc caatgggaag gttagaagat
    38281 gaacccctaa taattaaatg attcagtcac aatctaaagt gaatataaaa atctattgaa
    38341 agtttattta taagaattga aaatagtatc tattctatct ttagtgtttg tgagagggtt
    38401 ctggattata taagattact gtaaagagta attttgcaca ataagcatat ctgtaaatga
    38461 agagtttcgt ttatataatg attgtggctg aatcataact ataatagata gaatgtttat
    38521 aatagatgat tttataaaat actatattgt aatagctttt agctttcaaa taaatattag
    38581 caaaggatgg aaatgacaat gataaaacta aatgataatt tataaatatc tccttacgat
    38641 atataataat tgtagggaga tgtttctttt tttgtatagc ataatttttc tagaaattct
    38701 gtttcaatat gtaaaaagtt ggtatatata aatagtataa acagtgttgc taaaatatgg
    38761 tagatattat ataatataag tggatatgta tatacattat gagttatata atttgaattt
    38821 aataaaatag aaacgttgat ttatgttctg taatgtgggc accttggagc atattgagtt
    38881 agtggtgcaa ccggctatga aattgtattt atttataaat actattattt tcatagcttt
    38941 ttttatttaa aaaatggtta tatgttagaa ataaatttgg ttagtacgtt taaataattt
    39001 tacttagatt tttaaccaga tatattgtaa gtaagtatta attaaaaaat ttaaggggga
    39061 ataaaaatga agttaaaaaa gaataaaaaa ggtttcactt tagtggaatt attggtagta
    39121 attgcaatta taggtatatt agcagtagtg gcagttccag ctttatttag taatataaac
    39181 aaggctaagg tagcaagtgt tgagtctgat tatagttcag ttaagagtgc agcattatct
    39241 tattattcag atactaataa gataccagtt acaccagatg gtcaaactgg tttaagtgtt
    39301 ttagagactt atatggagtc tctgcctgat aaagctgata taggtggaaa atataaattg
    39361 attaaagttg gtaataaatt ggtattacag ataggtacaa atactgaagg agttacatta
    39421 acagaggcac aatcagcaaa gttattgagt gatataggtg aaaataaaat atatacaagc
    39481 actacaacaa atagtttggg aaatccatta acaagtaata caaaaataga taataacgtt
    39541 ctatatatag tacttataga taatactgtg atggacacaa caaaatagtt aaaacaatat
    39601 ttttataaaa atattgtaaa tttaatgaaa aatataatat aaaagacagg aagaaaatct
    39661 tcctgttttt ttatactatt ttctttaatg ttcaattatg aatgatgata taattgagat
    39721 agtaaaggag tgaaacctgt ggctaaaaaa gttagaatag gagacaaact ggtcgaaaaa
    39781 ggatatataa cagaagaaca gcttaaatgg gcactatcag aacaaaaaaa cagtggaaaa
    39841 agactaggag aatttctagt acaagaaggg cttatagata gtaacctact aataagtgta
    39901 ttaaaagaac tcttggatat agaaagtata tttttagaag ggacagaaat agacacttta
    39961 gctacaaaga tggttccaga aaatatatgc aaaagatata ctgtatttcc attcaaaata
    40021 gatggaaata aaatatgtct agccatgtca gacccacaag acagagaagc agttcaagat
    40081 gtaaggcgta tgtcaggtaa agacgtagag atatttatat caagtacaga agatataaac
    40141 aaagcaatag gtcatgctta tgcacattca gaaataaata aggcaatgac tgagtacaac
    40201 aaaaatagaa ctggtggagt aagagaaaca gtaatacttg aagaagatgt aaatgctgct
    40261 ccaatagtac gattagtaaa taacatacta gaaaatgctg ttagaatgga agcaagtgat
    40321 atacatatag aacaaagtga aaattacatg cgtgttaggt ttaggataga tggaatgtta
    40381 agagaatata tgaggatgaa ctcagcacca tataaggcag taatcagccg tatcaagata
    40441 atgtcagata taaatatatc tgaaaagaga ataccacagg atggtagaat ctatttaaaa
    40501 gtggataata aacccataga ttttagggtt tctacaatgc caactaacag ggatgaaaag
    40561 atagctatga gggttcttga taagagtaat tttatggtta gtaaagaagt actaggaata
    40621 gacgaacatg gctctaagat atatgatgaa ttgataaaca ctccttatgg tcttatattg
    40681 gttgttggcc ctactggaag tgggaaaacc actacattgt attcaatgtt aaatcaacta
    40741 aatacagaaa atagaaatct attaacaata gaagacccta tagagtatga attacctggt
    40801 gtaaatcaat ctcaaataaa tgaaaaggca gggcttactt tcgccagcgg acttagagct
    40861 tttatgcgtc aagacccaga tataataatg gttggagaga taagagatac tgagacagca
    40921 gaaatagcaa ttagagcttc tcttacagga catttagttt taagtacatt acatgcaaat
    40981 acagcagttg gagcaatttc acgtctttta gatatggatg tggaaagttt tttaataact
    41041 tcatctgttt taggtgtaat atctcaaaga cttacacgaa aaatatgtga gcattgtaag
    41101 gtttcatatg aagcagatat tggagaaaaa aaggctcttg gtattgatgt aaatgaatca
    41161 gtaactattt atagaggaaa aggttgtgaa aggtgtaata atactggata taaaggacgt
    41221 ctaggtatat ttgaaatgct tgaaattact ccagaaataa aagagttaat agattcatct
    41281 gcaaatcaaa gagaaatttt aaagatggct agaaaacaag gtatggttag cttaaaagag
    41341 gatatagtta aaaaggtttt aaatggaaag acaacagtag aagaaatgat tagaataatt
    41401 ttgatgactg attaaaggat gatattatga aaagttttaa atacacagta ataaaagagg
    41461 acggaaaaaa gcaaactgat gttattgaag ccaacgattt taatgaagct agaaatcttc
    41521 ttagaaagag aaatcttaga gttattgaaa tcaaagaaag caaaaataaa aatataagat
    41581 tatctagtat aaagagaaaa aaagacttag gagctgacca gataagccac ttttgtagac
    41641 aatttgctat tatagtatct tctggtataa atagtatttc tggattagaa acattagcta
    41701 gaaggtcaac taacattaca ttaagagaag aaattaatcg tatagtagca gaaataaaag
    41761 taggttctac tattgcagat tcaatgcttt catcaaaatc aaagtttcca aagttacttg
    41821 gtgctatggt agctacaggt gaagctacag gtaaattaga tgaagtatta aaaagtatgg
    41881 cttcatttta tgcaagtgaa cacagggtta aacaaaagct tagaaatgct gcaacatatc
    41941 cattaatagt tttgatttct tcatttataa tgatttttat atttacaaca tttatggtgc
    42001 ctaagatgat taattcaatt acaacagtag gagcaactct tccacttata acaagaattg
    42061 ttatggggtt tggtatgttt atgaagaagt tttggataat agtactttta gttattatat
    42121 tatttatcta tcagtttaaa aaatatctaa aaacgtcagt tggaagggct cataaagata
    42181 gagttataaa taagatacct gttttaggaa aaggaataaa ttgtatggta gctacacgat
    42241 tttcaagagc attgtatcta tttgtatcaa caggataccc attagttcaa gggcttgatt
    42301 atattataga tagtgtaaat aacactatgg cagagaagat tttagcatca gctaaagatg
    42361 gaataacaag aggagaaggc ttagcagaaa atttagagag atatacgtat tttgactctg
    42421 tgttggtaca gatgatagct ataggggagc agacagggga attagagaat ataagtagac
    42481 agatggcaga attttatgag tatgaatccg agatttattt aaataggatg gcctcaatga
    42541 tagagcctgt tttaataatt gcagttggta ttatagttgc gatactggtt gtatcaatat
    42601 tcatgccaat gcttagtata tatgatgcca tgtaggaggg aaaggacaaa tgaacaaggt
    42661 ttatgtatca tattatggcg attctatttc catagttgag ggcagatata aacacgataa
    42721 ggataagttt tatgtaaaaa attttaatgt gttttcaata gaggatattg cacaagattt
    42781 ttctaaggaa aagtatgctc tattgagata cgcattagaa aatgttaagt taaaatcaag
    42841 acatgtagtt ttttgtttaa atacaagaga tgtaatttta aaaccccaaa aacttcctaa
    42901 tatggacaaa aaggatttag acggatttat gaagctagag atggacgaaa tgatggcttt
    42961 ggagtcagat gaatatgtat tttcgtatga agtatctagt gaaacacctg ataattcaga
    43021 tggcgatgaa tcaagcttaa atcttattat tggagctata caaaaggaag aggtaaatgc
    43081 aattgtagag ataatgcatg aatttgattt agtattagat agaatagata cattatcaac
    43141 agcttactta agaatgctta aaaatttaga ctacaatgat ataatggtaa ccaatataag
    43201 tgacaatagc acaattataa atatatataa gaaagatact ttatttatta gtgataatat
    43261 acctataaag ataactgcta aagatatgga catgcaatta ttaaatgtag tagaagaatc
    43321 aaaaggtctt atgaattact attcgtcaag aaactttggt aaggttacgg atactatatt
    43381 aatattagga aaaagatatt ccaataaatt aatttatgaa acttttagct ctgcattttc
    43441 aagctatgtt tctcaaggcc ttactgagat aatggatata tctgatatga taactggaga
    43501 tatagatgaa gatgatataa attcaattgt agagctttta ggaagtatga tagaacctaa
    43561 agggaaaaga gattttgaaa atataaactt tttaccaatg gatatactta gaaaacagat
    43621 aaaagctaaa caaatgaaaa acatactgaa aattgctcca atagttattt tgattcttgc
    43681 tataccttat ttatgtttaa ttttttcaaa taatttagca aataaggaac ttgaaaatgt
    43741 taatggtgaa ataagaaata ttgaatctgc atattatcag attggaaata tagaaaaaat
    43801 gatacagagt aaaagcgaag aaataaaggt atatgatatg ttaattggga aaaaggtaaa
    43861 gtgggaaaat ttattggatg atatagataa aaatactcca agtaaagttg aactaacaag
    43921 tttatcaaca acttatcaaa atcttggaac taataataaa gctgataatt caaaaagtaa
    43981 taagactaca acagataaat caagtaacaa tgaaacaaaa tcaagtacga aaagtgatag
    44041 taatggagat aaaacagata atgatttaaa tgaagataag actacaaatg tgtcagaagc
    44101 attaactgga aatagtgaga ctaatagtaa taacacggat aaagatgata aaactccatt
    44161 gtatgataag ataccaaatg ttataaatat agaaggaaat gctaaagatt cctcttatgt
    44221 aggtcaattt ttatataaat taaagggatt atactatttc aaggatgtta aattacatag
    44281 cataaaaagt aatgcagaag attctaacta tacatttagt atgacattat acctgaaaga
    44341 aggtgtatta actaatgaat aaagatatat ttaaaaaaga tataaaagat atatttaaaa
    44401 tggaagttag tctaaaaggg attcttataa aggttataat tttattagct attttatcat
    44461 atggttattt tggactttac cctcaatatc agaaatatat tgctacaaaa aattctgtaa
    44521 aacaggctac tgaaaaatta aatatatata aagagaaaac tgcacttatg ccaaaatatg
    44581 aaaaacaact tgaagaagtt gaaagtgaat taaaagaaaa aagcaaaaat cttttatatg
    44641 atatgcaaga tggtcttttt ttaataggtt tagataaaaa aatgaaggct tataatattg
    44701 acttaaccag ttatgacgtt ggaaatgtta aagaatacga acatttttat ggtatatcaa
    44761 caacaataag tgttagaggt gattatagaa atgtcaaaaa attaatggct tatttagaag
    44821 aacaaaaaaa tgttactcaa atactagatt atacaatgac ttcatatata gaaccagaaa
    44881 aagaagagga agaaacaaca aacaataagg atttaattaa aaatgtgaac ataaataaag
    44941 atacaaaaat atattgggtg gaaggcgaaa ataagtggca tttatataag gattgtccat
    45001 tgataaaaga gggagagatt ataaaaaatg acaaactttt agataggtct actaattatc
    45061 tatgtaatac ttgtgtactt agagctgcaa cagaaaaaga agcaaaaaaa gaagtcgtag
    45121 ctccaaaggc agaaggtgta gtagatgcta catttaaatt tataatgtac actaaagaga
    45181 atactacaat gatacttgat acagataatc catcttcatg gagaccagga aaatataatc
    45241 catttaagtc tagtgcgaaa taaagctaag taagctttaa agataggtga tgagtttgta
    45301 caaaaataat gaaaaaggtc ttactttatt agaagtaata atagctgttt ttatactgac
    45361 aatagtttta agtatttctt ataaagtgtt caatggtata acatcagcag taaaaaagca
    45421 acagattatt acagatgctc aagtaaatat taatctaatt aataagtatc taaatagaga
    45481 tttggaaaac tgtaaagagc taactaaaac tggttcaggt aataattatg aatacaatat
    45541 agagatgcca gataatgttg taaaatatga agtttctata gaaactaaaa aaaatacaga
    45601 ggtatattct gtaacgagaa tccaaaacaa tacaattgat acagaaaatg aagttagaga
    45661 agagataatc tataatcaac cactagttca aaacaataaa gaaatgaagg aaacaccatt
    45721 taaaatagaa aagcaaactg gtaaatctat ttatactgta agtatatact ataatgaatc
    45781 agtgcaagaa agtcataaaa atagcaattt aaataacaaa acttatacat ttgatgttat
    45841 gtcaagaata ggataataag aatttattaa aaaataagta ggttgataaa atatgagtaa
    45901 aaagaatagt gaaagaggat tttccttaat tgaagtgtta gtagctatgg ctattatggg
    45961 aattgtacta tttgcatttt ttaatattat caatactaat aacaaagcaa acactaaaaa
    46021 tgacacagat ataacttcct taaactatgt ccaaagtgaa atagaaaatc taagagaaaa
    46081 gataaaaagt ggagaatttg attttgatag tttagataaa ctagaagatg gaactgtcgt
    46141 atatgaaaaa ttaatagata aatcaaaaaa agttgtatat gataaagttc ttagtgaagg
    46201 tgacgtgagc ttatatgata ctccatatga aaagattaca acaataaaag atgaagatgg
    46261 taatttaatc gacaaaggaa atattacaaa taagataaag actattgtag aagataaaag
    46321 tggtcagata tataaaatag ctgtaactgg gaaaagcatg aatgactatt caagtaaaaa
    46381 agaggttaaa attgtaactg aaatatttaa agataaataa ttttacttgg gggtgagatt
    46441 ttgaggaagt ggaataaatt taaaagtgaa agaggggcag ctttagtttt agttttggtt
    46501 gtagtagcat tgctttctat tgttggattg atattttcaa atcagatagc taatagaata
    46561 aaatctacaa agactactaa tgaaggtata caagcaaagt atttagcaga gacatgtgtg
    46621 gaaaatagta ttgataaagc atatgaaaaa ttgtatgatg agcttgagaa aatggataat
    46681 gagtttaaaa gtgaaaatca agaaaaatcc atatctaggt caaaattaag aaatatatca
    46741 gatgaagatt ttaataatca agatgaggaa aatattgaag cagaaagact tggatatatg
    46801 aataatataa atttctattt aaataaagct tcttcagact tggaaaaagc tagtatggaa
    46861 ttgaaaaagc tatatgattt agatatgttg gactatagag acattgaata tgtagatgca
    46921 aatattattt cacatagaga ttctatctta gaaatttgca agaattatac ttctggagat
    46981 attagtaaaa taaatgaata tattctgaaa gaagatatag actcaactac attgattgag
    47041 gcaaagttag tgaataatga tattctttta aaaaagtttt tagaagaaaa taaaattgaa
    47101 aatgaacatt taaattcagc cttttcacat acttataagg cattagataa catttctttg
    47161 gcgatgcaaa atatgattga gtatagacat acgtttcata tagatgagcc aaaagtagaa
    47221 gtatctaatg ggattccaga ctctcaacaa tattatgaac taatacaaaa tcctataata
    47281 aatagtatgg agtatagctg gaatagtaaa tgggatacat tagaaaacct attggaaatt
    47341 ttaccaaatc aaactcaagg atttaattct ttaagagttc atttgagaaa taatgtaaga
    47401 aaatttgaga agttaagcga taatatatct agtggtaaaa aaaatacagc aaaaaatttc
    47461 ttaaagtata aagagttatt gtatgaaata tcagaccaat gtaatcaact aaaaagtatg
    47521 tcttatgaga aaatacctgt taaatatgat aacatggctt tgataacaac ttttgactat
    47581 atacaaaatg agcttttagc agagataaaa tgcagattaa aagaattaaa accacaagaa
    47641 attgataaaa cagaaggtat tactataaaa ataccttttt ataaggctga ctacgacatg
    47701 acaaaagaag gatggcctaa attaaaagaa aatggttcag gggcggagtt aagcttaatg
    47761 gtaactggtg ataaaaatgg aataaaagaa gtggaagtta cagatggtaa aaaaaatatt
    47821 attggtttag gtgttgaaga aaattcaaat tcaaagtata aggttgacgc cattgtaaac
    47881 tttaatctaa atatagatac aaatgtagtt ggaaattatg atataaaaga taaaattttg
    47941 attaatcatg atataagttc atacaaaaaa gtaaactaaa tataagaagc aacttagtat
    48001 aaataacaaa ataattttat tatttactac taaaaaaata agtaaactaa tagaagatat
    48061 ggagagtaaa tattatgtat atttatgatt tattagagca aggtataaga ctaaacgcat
    48121 cagatataca cataacagtt ggaacaaatc ctgtagctag agtaaaaggt ggatttgtaa
    48181 aactaagtga acaaatattg acatcagaag taacaatgca aatggcaaaa gacatagcag
    48241 gggaatctat gtttaaagta atagaagaac atggagaagc tgatttttca gcttctctaa
    48301 aaacaggaga aagatttagg gtaaatgcat ataggcaaaa aggaaattac gcaatagcaa
    48361 taaggactat aacagcagaa attccaacat ttgaaaaatt aggccttcca gagagtataa
    48421 aatcttttac tgaaaagcat aaaggattag ttttagttac aggtccaaca ggaagtggta
    48481 aaagtacaac acttgcaagt atgataaata taataaatga aaaacaacaa aaacatataa
    48541 ttacacttga agacccgata gaatatgtac atcatcataa acaaagttta gtaaatcaaa
    48601 gagaagtagg aactgataca gaaagttttc attcagccct tagagctatt ttgcgtcagg
    48661 acccagatgt aatactaata ggagagatga gagaccctga aactgtttca atagcattaa
    48721 cagcagcaga aacaggtcat ttagtatttt caacactaca tacagtaggg gcagctaaga
    48781 caattgatag aatagtagac atgtttcaac catcccaaca gcaacaaata aaaacacagt
    48841 tatctacagt ttgtgaaggt gtggtatcac agcagttact tcctacagca gatgggaaag
    48901 gcagaatagc agcaattgaa ttgatgtttg ctactccagc aataaaaaac ctgataagag
    48961 aaggcaaaac atatcaaatt ccaaacatga tacaaactgg agttaaagta ggtatgaaaa
    49021 ctatggacca agatttaatg gaactttata aaaatggaaa gattacaaaa gatatggcac
    49081 ttagtaggtg tactgaccaa gagtttatga caagaatgat aggtggagta aactacaatg
    49141 gatattataa tcgttaatct atatgtattt atagtgggta tagtgtttgg aagtttcttt
    49201 aacgtctgta tttttaggat tccagaaggt atatctattt ctagaccacc ttctcattgt
    49261 atgagttgta atactagact aaagccaata gacttaatac cagtgctaag ctatttaatg
    49321 agtggcagaa agtgtagata ttgttatgag aaaatttcta gtagatatgc tatagtggaa
    49381 ttaattacag gacttttatt tttaacagta tttatgttat atggaatatc tacatcaact
    49441 atttattatt tggttcttgt gtctttactg attataatta catttataga tattgaccac
    49501 tttataatac cagataaaat attggttttt ggagctatat tttctatgat ttttaacctt
    49561 ttatttaaag aaattccagt taaggattca attctaggtg tttttatttg tggaggaagt
    49621 gtctggatta tagttctttt gattgaattt gtaataaaaa aagagtgtat gggtggagga
    49681 gatattaaac tatttgcaat gctaggcctt tacatgggtg ttaaaaatgg tcttttgaca
    49741 gccttactaa gcgtttatgt aggagcagtc tatggaatct gtgtaataat tataagtaga
    49801 ataaaaggaa aggaatataa ttctgttata ccatatggac cttttatatc tattggagca
    49861 ttaataacaa tcttatgtgg aaagaaatta cttgaacttt atttttctat ggttatatag
    49921 atgatagtca agttaaataa agttttaatt taaaaaaatg aattaaagta tttaataagg
    49981 acaatattat tattaaccct attactaaag tgctacaaac tatggagtat ataataataa
    50041 tttccttatt ttttgcacat ttgataaata aatctctatg tgttttttgt gatagagaaa
    50101 agaaaaattt atacaatacg ttttttattt acataataac tgtagtttta tctgtattaa
    50161 tatatctaaa atttgggatt agtgtagaat gtgtacaata tatggtttta attccatttt
    50221 taataattat atccattgta gattactaca caatatatgt atatgatata acaatagtta
    50281 gtggtataat agttcaaggt ataatttttt tggccttttt caatatagct caatttgatt
    50341 atataaatca tatatttgga ctattgatag gctttattat accttatatt attgctaaac
    50401 taacaaaagg attaggctat ggagatgtag gtctctatag cttatgttgc tttgcattgg
    50461 gaaataatta tgggttttat gtgatagttt tatcatttat tttagcgttt ttgtttttta
    50521 ttattttata cataactaaa gtatttaaag atgtagatat aagaaagcaa attcctttta
    50581 caccttttat ttccttgtcg acaattttaa taatatttac agagtataat atatttaatt
    50641 tttattataa tttgttatac aaatttatat aacaggaggt aagaaatgta cgtagtagta
    50701 gggttaggta atccaggaaa gaagtatgag aagactagac ataacgttgg atttgatgtt
    50761 atagatattc ttgctaaaga atacaatatt agtgttacta aaataaagca taaagctttg
    50821 attggtgaag ggagagtagg tactgaaaaa gtattattag ttaaaccaca aacttatatg
    50881 aatttaagtg gagaaacatt aatagatata tataaatatt ataaggttga tttaagtaat
    50941 atagtagtag tatatgatga tatagattta gaagttggaa agatacgtat aagaaaaaaa
    51001 ggaagtggag gaactcataa tggcatgaaa tctataacta attgcctagg ttcaaatgac
    51061 tttccaagag tgagagtagg tgtatctaaa ccagaagctg gtcaagactt agcagacttt
    51121 gtactttcta gatttagaaa agaagaatct gataacataa atgaagcatt agaaaaagcg
    51181 gcagatgcta ttgactcaat aataagagaa aatatagata tgtctatgaa caaatataat
    51241 ggatagtgag agggagattt aaatgaatga tgttttttta tatcctttgc aaaattcaaa
    51301 agagtataaa gatataataa actgtataaa aaatacaaaa ggctcattat tggttaatgg
    51361 acttttacca gtacaaaaac ctcatatatc gtattctata tttaatgatt tatcacgaca
    51421 aatgattttt ataacaagta gtgatttaga agcaaagaaa gtatacgaag atttaagttt
    51481 ttatatggaa gataaagttg aatatttagg ttttcaagat atttattttt atcacttgga
    51541 tgcaaaggac agaaacgaag aagcgaagaa attaaaagta ttgttaaaac tagcaaataa
    51601 agaaaaaatc attttagtaa cttctattga ggcagtttta agaaaataca taccaaaaca
    51661 agttttgctc gatagtgtat ctcattataa ggtgggagat agcttagact tagaaaagtt
    51721 aactgagaaa ttagtttcat tgggatatga aagagtttca aaaatagagg ggtttggtca
    51781 atttagtata agaggtggaa ttattgatgt attttcactt gaatatacaa atccaattcg
    51841 tatggaatta tttgatgatg agatagattc catacgaact tttgacgtat attcacaaaa
    51901 atcaattgat aaattgcaac aattctctat aactccatct agggaattta tttatcccga
    51961 aaaaactaca gatgcattag ttaaattaaa aaaagaaaca actaaaaata ctgatgaagg
    52021 tacatttcaa aatatagatt atatatcaag taagacttat tttgaaggtg tagagaacta
    52081 tatagattat atttatccag aagaaaacaa aagtatattt acttatttag cagatgatgc
    52141 aatagtattt attaatgata taacgagact taaagaaaga tgcgaaaatt atataaatga
    52201 gtttagagaa aattataaat taaatcttga aagaggtctg gctattaaaa accaaggtaa
    52261 attgttatat cactatacag atttagagta cttagtaaaa gacaagtctg tggtattaaa
    52321 ctcgctattg ccaaagtcta taaataactt ttctataaaa tctataataa attttgaaag
    52381 tagagaagtt cctacattta atggaaaggt agatttgctt gtagaagaat taaatcggct
    52441 gaaatataat ggatacaaaa taattttagc aacaaatacc ttagaaagag caaataaatt
    52501 aggaaaagat ttattggaca aaggcttaga aacgaccatt tctaaagata gagatattga
    52561 aattaaatct tcacaagtta taattgtacc tgcacacata aacagtggtt ttcagtataa
    52621 atctataaag tttgttgtta taacagataa cgaaatgata ggtgtttata aaagagcgtc
    52681 taagactagc aataaaaaag ttaaaaaggg aaagaaaata gaatcctttt tagatttaag
    52741 tgttggagat tatgtagtcc atgaaaatag tggtgtaggt agatacacag gaattgaaca
    52801 aattacagtc aatgctatta aaaaagatta tatgaaaata gtatatcaag gtggagataa
    52861 tctatatgtg ccaatagacc agatggacaa agtacaaaaa tatattggtg cagaagtaga
    52921 aaaagttaag ttaaataagc ttggaacaaa tgagtggacg aaagcaaaag caaaagttaa
    52981 aaaagaaatt gaagatatga caaaagactt gattgagctt tatgcaaaac gtgaaaaaat
    53041 acaagggtat aagttttcaa aagatacacc ttggcaggca gagtttgaat cgttgtttcc
    53101 atatcaagaa acagaagacc aattaaaggc tatagaagaa actaaaaaag atatggaatc
    53161 aagtaaggta atggataggc tagtatgtgg agatgttggt tatggaaaaa cagaggtagc
    53221 tattagaagt atttttaaag cttgtatgga ccaaaaacaa gttgctgtat tggttccaac
    53281 aacaatattg gcccaacaac attataatac attcaaagag aggtttgaaa attatccttt
    53341 gagagtagaa gttttaagta ggtttaaaac accaaaacaa caaaaacaga ttattgaaga
    53401 tgctaaaaaa ggattagtag atattttaat tggaacacat agaataatat ccaaagatat
    53461 aaatttacca aacttaggtc ttgtagtcat tgatgaagaa caaagatttg gagtaaaaca
    53521 taaagaagct ttaaagaaaa ttaaatctac agtagatgta cttacacttt cagcaactcc
    53581 tataccaaga acattacata tgtctttaag tggaataaga gacatgagtg ttatagaaga
    53641 acctccacaa gaaagacatc ctgtaataac atatgttaca gagagtaagg aaagcgtaat
    53701 tcaagatgag atagagagag aattaagtag aggtggacag gtattttttg tatacaatag
    53761 agttgaacac attgaagaaa tggcaagtat gattcaaaaa ctcgtaccag atgctagagt
    53821 ggctgttgca catggaagaa tgacttctaa atcattagaa aatataatcc taggattttt
    53881 aaataaagat tatgatgtac tagtctgtac tacaataatt gaaactggta tggacatatc
    53941 aaatgcaaat acaatgatta tatatgatgc agataaaatg ggattggctc aactttacca
    54001 attgagaggt agggttggaa gaagttcaag acaaggatat gcatatcttt tgtatgaaaa
    54061 agataaaact ttaagtgaga tagcggagaa gagattaaaa gctatcagag aatttacaga
    54121 gtttggttct ggttttaaaa tagcaatgag agaccttgaa ataagaggag caggaaatat
    54181 tttaggttct caacaacatg gtcatatggc agttataggt tatgatttgt acgttaaaat
    54241 gcttaatgat gcaattaaaa aagtcaaagg tgagcctatt gtggaggaaa ttgatgttga
    54301 aatagattta agtgtaaatg catacattcc agataattat atcaaagatg agttgattaa
    54361 aattgaaatg tataagaaaa tagcttctat tgaaaacaaa gaagacatgt tggatataca
    54421 agaagaactt gaggatagat tctctgatat acctaaacca gtccagacac ttcttacaat
    54481 agcttatata aaatcgttat gtaagatatt aaaaatagaa aagataagac aattaaaaga
    54541 tgaaatatta ttagttccaa taacaaagta tagaactaaa cagaaaatag gttataatat
    54601 agttacagaa ttagaagaat tattagaaaa gatgtgcaag gtaaaataac ttgtaaattt
    54661 aagaagggat gttttaaatt tgaaaaaagt aataacattg gtaatagcta tgattttggt
    54721 agtatctgtg actgcttgca gttctagtaa aggagaaact gtggcaacag ttgaaggtac
    54781 aaagatatca tcagatgaat ttaaaaaaac tatagctctt tataaagatt ctatggaaca
    54841 aacttatgga aaagatatat gggataagga agttgaaaag ggtgtaaaat acaaggacaa
    54901 gtttaaagat ttaattcttg accaacttat aactacagaa gtaatatatt ctcaagctaa
    54961 aaaagataac ttacttccta aaaaagagga tgttgaaaaa agttttaaag aattaaaaga
    55021 cgctatgggt aaagatgaaa aatataaaga acaacttaag aaattaggta tagatgatga
    55081 gtttttaaga gaccaacaag agaaagattt agcaatgcaa aactatcaaa gtaactttgc
    55141 taaaaagact aaaatatcag atgaagaaat gaaaaaatac tatgatacac ataaagatga
    55201 atttaaaaaa gatgaggtag aagcttctca tattttattg aaaacagtag atgataataa
    55261 caagccactt tcagataagg aaaaagctga agctaagaaa aaggctgaag aggctcttaa
    55321 agaagttaaa tcaggtgagg attttgcaaa agtagcaaaa aaatattcac aagatacttc
    55381 agcaagtgat ggtggtaaat taggattctt ttcaaggggt caaatggttg ctgaatttga
    55441 agatgctgct ttttctatga aaaagggtga agtatctgat ttagttgaaa ctcaatatgg
    55501 ataccacata attaaagtga cagacagaat aaatgagcaa acatcatttg aagatgctaa
    55561 agaaactata aaagaccaac tgttgaaaaa taaatatcaa gaacaaatag aaaaattaac
    55621 taaagaagct aaggtagaaa aagatgaaaa agtaataaat aaaatcacaa tctaagactt
    55681 aaaaaaagag taatatgctt aaaaaagcta tatctggata agatatagct ttttttgtat
    55741 atttttctta acaatggtaa aaataaactt aaagtaattt atggagggat taaaaaatat
    55801 gaaagcaaca ggtatagtta gaagaataga tgatcttgga agggtagtta tcccaaaaga
    55861 gataagaaag acattaagaa ttagagaagg agacccttta gagatattca cagcaaaaga
    55921 tggagaagtc atacttaaaa aatactctcc aataggtgaa ttaaatgaat tttcgcaaga
    55981 atatgctgaa acattagctg aagtactagg atatggagtg ttgataacag acttagattc
    56041 tataatagct gtgtctaagc ttcctaaaaa agattataaa gaaaaaagta taagtgatga
    56101 gttagaagca ataataagtg aaagaacagt aaaatattca aaagataaaa acattttacc
    56161 attattcaag gaagaccaaa aagaatatac tagtcaagtt atcatgccta taattagtgc
    56221 atctggtgac tgtataggtt caattgtagt tgtttcaaag gataaagata caattgcaga
    56281 aagtgaagaa aaatctttaa aaattgctgc taacttttta ggaaaacaag ttcaataatt
    56341 ttatcataaa gaccttttac atgaaggtct tttgaagttt ttgtgtaaaa tattgaatat
    56401 agcattaaaa atatggtata ttaaagtttg ttatataaac agtatggagg ttatgttatg
    56461 aacaataaca aaaataaaga ctcctttcta aaaggagccc ttatacttgg gctagcaggt
    56521 gtaatcgtaa aaataatggg tggatttttt aggattcccc ttggaaacat gattggttca
    56581 gaaggaatgg gatactatca ggctgcttat cctgtgtata ctctattttt aacattagct
    56641 actgctggat ttccaacagc tgtggcaaaa ttggtttcgg aaaaagttgc aattggaaat
    56701 tttaaaggtg caaataagat atttaaggta tcacatacag ttttattcat taccggtata
    56761 atatcatttt gtatattatt ttttggagcc gactacatag taacaaacgt aatgaaaaat
    56821 ccaggtgcgt tatactctat gaaagccatt gctccagcat tactatttgt tcctgcaatg
    56881 tctgcatata gaggatattt tcaagggaga caagatatga ctaagattgc tgtgtctcaa
    56941 gttgcagagc aattttttag agttgtatta ggtcttactt tggcttactt gcttatgaag
    57001 agtctaggtc agacttttgg tgcagcaggt gctatatcag gggctacaat aggttcaata
    57061 gcatctatgc tttatttggt gtttgcttat atgctaggga gaaaagagcg tagagcagag
    57121 atagatgcaa gccaaagatt taaagatgaa agagtgtctt atatttttaa aaagctttta
    57181 actgttgcca ttcctataac aataggagca tcagtaatgc cacttgtaaa tatgatagat
    57241 aatgttattg taataagaag acttatggaa gcaggcttta catacaaagt ggcaaattcc
    57301 atgtttggtc aattaacagg tatggcaatg gccacaataa atttaccagc agtcataaca
    57361 actgcaatga gtatgagctt agttccagca atttctaaag catatgcatt aggaaataag
    57421 tcaaaagcta gaaaagatac taagagtgca gttaaggtaa ctttacttat agtattgcct
    57481 tgtgcgtttg gtatggcttc tttagcaata ccaataatgg gtcttttatt tccacatgaa
    57541 ccatcttctg taggaactat tttgtttact cttactccat gtgtattatt tttaggatta
    57601 atacagacat tgactggtat aattcaggga atgggaaaac caatagtacc tgtaattgct
    57661 ttatgtgttg gtatgctatg taaaattgta ataagttata ctttaacagg tataccagat
    57721 attaatgtac tgggttcagc atttggtaca gttacagcat actttgtggc agccatgatt
    57781 aacttactat atgttaaaaa acacatgaat gtaaactttt ctaaaaaaga atttataata
    57841 aagccattta taacagttat gacaatgttt ataatggtta agttgagtta tggtgcttta
    57901 gttggattct taggaaacag catatctaca ataattgcaa tatgtgttgg tggaattgta
    57961 tatgtagtag taatacttgg aataggtgga attaaaaaag aagagattct aactatgcca
    58021 aaaggtgata aattatataa attgttaaaa aaagttaaac ttataagata atataggaat
    58081 atatatgcaa ataaaggaaa tactatacaa atagtaaatc tccttatttg tataataata
    58141 taaaaataaa gtgaaatttg ctaataggag tgatgttatg ggtaagataa gtattgtagg
    58201 tcttggacca ggagactatt ctcttataag ccaaggtgtg ttagaatctt taataaatag
    58261 ccctaaagtt ttttttagaa cagagaaaca tccaataatt gataaactta aagaaactat
    58321 acaatacact tctttagact acttttatga aaaagaggaa aattttgata atgtttattt
    58381 gaaaatatct gaatttatca tagaagaatc agcaaagggt gacctagtgt atgctgttcc
    58441 tggtcatcca agagttgcag agaagactgt aagtataata gagagattag ccaatgaaaa
    58501 gcacatagca gtagaaatat tgccttctat gagctttgta gacgctatgt ttaactattt
    58561 ggctatagac ccatctgagg gatttaaact ggtagatgct tttgaaatag aaaattcata
    58621 tatagacttg gatacaagta tgataataac tcaggtatat gataaattta tagcatctaa
    58681 tgtaaagtta aaacttatgg aacattatga ctataatcaa gaagtatgta tagtaaatgg
    58741 agctggtgtc aaagatttag aaagtaaaaa gtttataaag ctttgtgaat tagacagaaa
    58801 tgaaaacttt tttgactatt taactagctt atatataccc aaaagttcta aaaagatgta
    58861 taatacagta catgacttag aaataatagt taatacactt agaagtccat ctggatgtga
    58921 atgggataaa aaacaaacac atcaatcatt aaaaaattct gttatagagg aagcatatga
    58981 actttgtaat gcaattgata ataatgatat agatgaaatg gtagaagagt taggtgatgt
    59041 tcttttacag gttatattcc attgtcagat aggaaatgag gagggctact ttgatttaaa
    59101 agaagtagta aatggcatat gtaaaaaatt aattcataga catcctcata tatttagtgg
    59161 tgcaaaatta gatatgtcta agtttgagaa aacttgggaa gaattaaaga aagaagagaa
    59221 aggcgaaagt actataacag atggccttag aaggatacca aaacatttac cagctcttat
    59281 gaaggctagt aaagttcaac ataaagcagc acttgtcggc tttgattggg acaatgtaga
    59341 agatgtattt aaaaaaattg aagaagaata tcaggaatta cttgacgaac acagggtcgg
    59401 gaatataaaa tacataaagg aagagctagg cgatttatta ttttcgatag taaatctagc
    59461 tagattttta gacatagact caggagaagc cttgaactgt actacagaaa aattcataaa
    59521 tagatttgac tttatagaaa agagtgcaat gaacttgaat aaaaaattag aagacatgac
    59581 acttgaagag atggatgagt tttggaatca ggctaaaaaa caataaaatt gtggaaggaa
    59641 atttgtcaaa aatctagaac ttttatcaca aagatagaag tctatattat aaacattttt
    59701 aggaggtaaa tatcgtgaat aaagctgaat tagtatcaaa gatggcagaa aaaagtggat
    59761 taacaaagaa ggaagcagaa gctgcgttaa acgcatttat gagttctgtt caagatgcac
    59821 tagtaaataa tgaaaaagtt caattagttg gatttggaac atttgagaca agagaaagag
    59881 ctgctagaca aggaagaaat ccaagagatc cagagcaagt tatagatata ccagcttcta
    59941 aagcaccagt tttcaaagct ggaaaaggat taaaggatat aataaatgga taattagaaa
    60001 tcttgtgaaa aagtgtggat aaatttgcca cactttttct atagaaccaa ggaaggtgat
    60061 aattatgaga ttagataaat ttttaaaagt atctaggatt ataaaaagaa gaactgttgc
    60121 taaagatgca tgtgataaag ggattgtcac tataaatgga aaagttgcta aatcttcttc
    60181 agaggtgaat attggagata cattagaaat acaatttgga gagaagaaga tgaagtttaa
    60241 gataaatgaa gtaaaagagc atgtattaaa aaatgatgca aaagaaatgt acgaaatttt
    60301 agaataaaat taatagtcct ctagtatatt aatagtagta ttattgaaat atttactggg
    60361 gggatattat ggaacagaac ataactttga aagatagatc aaagctggtt atttcgggag
    60421 tcgagcacat atactctttt aatgacaaaa gagtagagct caaaacttct gtaggcgaga
    60481 tggtaattga aggcgaaaac ctagatatga gcaagctaag tatagacgaa aatataatta
    60541 gcatagatgg aactataaat tctatggtat atgcaaagcc taaaaagcca caagagagtt
    60601 tcttgaaaaa ggtatttaga tagatgatac cttttactca agatgtaagc attttttatg
    60661 ctactattta tggtggaata ctcatagggg tgttgtttga tttttataga gggctaagag
    60721 ggaattttaa attcatcaat tattttgcta taatatttga tgtattgttt tggtttctag
    60781 ctacggttat aatattcgta acaataaacc ttacagaatt ttttgattta agatattatc
    60841 attttgtggc tttgtttatt ggttttattt tatactataa caccataagt aaaattgttt
    60901 tatcaattat caataaaata attcgttttg tgagaaattc ttttaaaaaa gttacacatt
    60961 acatagtcag ttttttaaat aatttgtatt acgttataat ctattcacta cacctactat
    61021 ttgatattat attttatata ccaaacatat ttatagcaac aagaaaaagt ataaaaagaa
    61081 aatcaaataa aaaattaaag aacaagaaaa aatcaaaacc taagaagaaa aaaaataaaa
    61141 ctaaaaaaag ggtgtaggaa aaatgaactt aagaaaaaaa ttttcaggac agattattgt
    61201 tatcagttta tttttaggaa tatcaatttt tagtatgatg acaggatttg tatttgagta
    61261 tacaaaagct aaggaatata aaaaagaaat agcaagtcta aataaacaat taaaaaagac
    61321 tgaaatacaa ataaacagct taaagaaaga tgaaaaatca tatgaaggag acttagagga
    61381 tatagcaaga aaaagattga atatggtaaa accaaatgaa acagtttatg tagacataaa
    61441 tcgttgatta aaacttgcta ataagtttac agatgtattg attttaaaat ttaagattta
    61501 ataaaaaagt cattcagtta aggatggctt tttgttttta ttgaggaggg aatttcataa
    61561 tgaaagtagg agctatagat attgggacaa actcaatgag attattgata gcagattata
    61621 gagatgggaa ttttataaac agaaaaaaat ttgttaatac aactagaatt ggttatggtg
    61681 tggatagtaa aggatatata aaagaagatg ctatcaaaag aaatataaaa gcattaaaag
    61741 aattttctga taagtgtaat gatgaaaagt gtgaaaaaat ttattgtatg gggacatctg
    61801 ctcttagaga cagtaaaaat ggagattact ttgttaaatt agctaaagaa gaaactggaa
    61861 tagatgttga tatagttacg ggaatagaag aatcaaattt gggctttaag ggtgttttag
    61921 aaggaataca aggtgatgat actattattg ttatagatat tggaggaggt tcaacagaat
    61981 ttattgtagg tgattcagaa ggaattaagt ttaataaaag tgaaaatgta ggtgctctta
    62041 ggatgacaga gaggtttttg aagcacgatc ttattgaaga tgaagagttt gaagctatga
    62101 gttcatttat atatggtgag attgacaaaa cgttaaatca tataaaagac agaggattaa
    62161 aaactcttat aggtatagga ggtacgatta catcattatc agcaatgaat caagaattag
    62221 agatttactc tatggaaaaa atacataata gtaaaataca caaaaaagaa atagaagaaa
    62281 ttctacaaaa attaataaag atgacattaa gtgataaaaa gaacttaaaa gggctacaag
    62341 aaaaacgtgc ggatattata actgctggtg taaaaatatt gaatataata atggaaaaat
    62401 tagaaataga agaaataacc gtaagtgaat acgataattt ggaaggctta atatgtcaaa
    62461 agtcaaaaaa aatgtattaa tattttttga tttttaaaac cgattatgac aaaaaaaatt
    62521 actcctcttt gttacaattc ttaatataaa caaaagagga ggttttttta tgcaaagaag
    62581 tgtagcagtt gtgaagaata agactgtaaa accaaatgtc ataaaattaa taagcggata
    62641 tattgatttt actacggcgg cattcgctgt gatgggattt ttacttagta ggtctattat
    62701 agtagactct gtagctcctt taggaattgc cttttttata tgtgcatcaa agattgataa
    62761 gtacaagata cctgtattca tatcaacatt attaggaata ttgttatcat ctaatgattt
    62821 tggaaatgta gcaaaatatg ctgtgtgcct tacaatattt atggctatga ataaaaaatt
    62881 aaaattatca gattcaattg ctaaggttgc atttataggt gcaataatac tacttccaat
    62941 ctccatggga caagctttct tttcaaatag gtatgtatat ggtcttctta tagcaggagt
    63001 agaatcaata ataatgttta tatctatata tgtgttttct tatgggatag gtttaatggc
    63061 caatgtcaac aataggatgt ctgtacggac agaagaagcc atatcaataa gtttactgct
    63121 aacctttagc ataatgggaa taggagatat atctctgttt ggaatatcaa taaggaccat
    63181 tttatcgacc gtattgatat tgctgacctc aatagtaggt ggagctacca tgggagcatc
    63241 tagtggtgta attattggtg tagcatccat tttaaataat atgacatcag ctgtttatat
    63301 ggggatatat tctttttcag gattaataag tggagcattt aataaaataa ataaatattt
    63361 ctgtatacta ggatatattc taagttggac aataatatac ctatatactt ctggtattac
    63421 ttctaatatg atgcaattaa gagatatact attaggttgt ttgatagtac tagtattgcc
    63481 agagcgatta tttaataaga ttgaaaaatt aataaaaagt aatgtggctt caaatgaaat
    63541 cgtatacgat tatattatga gaagcaaaaa cctaactaat agcagattga acagtattta
    63601 taaaacttat gatgaccttg cagatacatt tgataagata agagaaaaag acaaagtgct
    63661 agaccaaaga gacatagcta atgtaataga tatgattcat aatgatgaat gtaaaagttg
    63721 tagtatgaga agaatgtgct gggagtcaag atttaatcat acatacacta tgatatataa
    63781 cattcttgaa aaaattgagg agaaagggga gttaagtcta aacgatatac ctaaaaattt
    63841 tagaaaggaa tgtatgaaac cagagtcaat agtgaaaata tcaaatcact attacaaaat
    63901 gtttgtatta gattatgatt ggagtgtgaa gttttcagaa agtagaaagt taatagcaaa
    63961 tcagataaga agtatttcaa aatccattaa atccttatct caagatttag aaggcgacat
    64021 aatgctagat atagaaaagg aaaaaaatat atatgaacag ttagaaagat atgatataac
    64081 agttgataaa gtaagttact taacaaaaag taacagtgag tttgaaatat caatagaaaa
    64141 gaaaacttgc catgatggat gtatgtgtga agataaaatt gtaaatataa tttcagacct
    64201 agtaggggaa aatatgtctg ttagaaaaat aggatgtcac tgtttaggag gtaaatgcaa
    64261 ggcaacattc gttaaatctc aaaaatataa ggctgttaca gaagtttcag caatgtcaag
    64321 ggatgggcat atcctttgtg gagacaatta cacatacatg gagataaatg atggtaaata
    64381 catgatggct ataagtgatg gtatgggaaa aggtaaaaaa gcatatgaag aatcttctgc
    64441 cactattgat atactggaaa aaatgataga tgcaaaaatt aaagatgaga tagtcattga
    64501 tactataaat aatatgttgt tgttaaagtc ttcagaagag atgttttcaa ctctggattt
    64561 gggaatttta gatttaaaac gaggttgttt agaaactata aagatgggag cgtgttctac
    64621 ttatataaaa agagaggatg gagaagttga cttaatatcg tcatcatcat tacctgtggg
    64681 tatattatca gatgtaaaaa ttgatagaaa aaatgtaaaa gttaaggaag gagactatgt
    64741 tataatggtt tctgatggaa ttgtagatgc aggaagaaat aacaatttag gagacaattg
    64801 gttaatatac ttcttaaaga atatagagac tacaaatcca aaggaaattt caaatttaat
    64861 attggataga gcattagagt tacaagcgtt acaaattgaa gatgacatga ctgttttagt
    64921 tacgaaaata tgtacaaaat aaggtttaca agattatgtg tataagatgt ggataacttg
    64981 tgtataatat gttaatagac ttttgaaaaa agtatagtta ttaacaaaaa taacatataa
    65041 atcaacaaaa tttgttgata agtatggcag aaatgttaat aaaatgtaaa aaaagaaaaa
    65101 acaaaagcta ttatcaaaag agattcatgt tgaaataact aaatttctaa gtaaagaaaa
    65161 cctgtgaata tgttgattat tatgtggata actattaatt cgatatttat ttatatagta
    65221 tgaataattg aataatatat ctaaaaaaag ccatcctatt attaagtaaa cttattgaaa
    65281 ggatggcttt ttatgaatag aaaattgtgc aaagcaataa tacctttatt tatttctatt
    65341 ttttgcatgt taataataag tataaaagct gaccaaggat atcaaaagaa taatgacaga
    65401 gcaattactg tcatgaataa taatgatact gaagatgatg ctgaaaaaaa acaagaaata
    65461 gactatgaaa aaacatgttg gaatttgcaa tctttgttta aaagtgatga ccaatggaaa
    65521 aaagaactta agagttttga taaagatact aaagagttga aaaattacat aggtaaagta
    65581 acaaaatcgc caacacattt gtcttttgcc ttaaacatta aagaaaaact tgatataaga
    65641 ctaaataaac tttctgccta tgtaaaatta aaacaagata ctaataaaaa ttcgtataag
    65701 tacttagata tgaatgatag tatgagtaaa tcctatggga attatatggg gatttgttca
    65761 gatttagagt tagaaatact aaaattatca aacaaagatt acaagaaaat tatttcaaat
    65821 aaaaatataa ataggaaata tggtgcatat ttaagagata taagaaggaa taaagtacat
    65881 tatttggatg ataaatcaga agatattcta agcaaagtat cttcaataag tagtcttcca
    65941 agccaagtgt atgaattatt tagaaatatg gatagaaaaa ctaatttgac accagctcaa
    66001 tatgcgagtg aattagagtc atcagataga gaaaatagaa aaaaagctta tcaagatgaa
    66061 cttataactt acaatgataa tataaataca attgcaggtt taattactgg tcaagttaaa
    66121 aagaatattt tttattctca ggaaagaggt tatgagtctt ctttagaaat gtacctggaa
    66181 tctgataatg tagatgaaaa agtatacaat aatttgataa ggactgtaaa tagtaatatg
    66241 gacagtctac acaaatatat agatttaaga aaaaaagtat taaaattaga taaggttcat
    66301 tcatatgata tgtttgtgcc tatggttaag gctgttgata ataatattaa ttatgaaaag
    66361 gcacaaagta tagtatattc agcgttgtca cctttaggaa aagaatacaa tgatgtagta
    66421 tataaagctt tcaatgaaaa atggatagat gtttattcac atgaaaataa agtaagtggt
    66481 ggatactgct tatctgttta tgaaaatcat ccatatatac ttttaaatta taataattca
    66541 ttaggttcag tttcgacttt atcacatgag cttggacatg ctgtatatga atatttaagt
    66601 tctaagaatc agaattattt taattcaaat ccgtctatat ttactcacga agttgcatct
    66661 acaacgaatg aagcattact ttacgagatg cttataaaag aagctaaagg caatgatgaa
    66721 aaagcttatt atataacaca gtatttagac ttgataaaag atactcttta tacacaaact
    66781 atgtatgctg aatttgaaaa aactatacac gagttagtag aaaaaggaaa aagtgtaaac
    66841 gcattagtat taaatgatgt ttggggacaa cttcttaaaa aatattatgg acaaagctat
    66901 gaattagacc aattatctaa ggtcggatgg gctagaatcc cacattttta taatagtttc
    66961 tatgtctata aatatgctac aggttgtagt gcaggagtaa gttttgccca agacatccta
    67021 aagaatggtt cagataacta tatgagcttt cttaaaaagg gaagttcaga ttatccaata
    67081 aacttgttaa aatcaagtgg cataaattta actaatacaa aacccataga aaatacaatc
    67141 aaaaaatttg atagtttagt agcagaactt gaaaagctat tataaacatt attgtaatcc
    67201 acatggtaaa tatatcgaat taatgataaa atatactaaa agtttaaagt cctatcaaga
    67261 taggactttt tatttattaa ttagtgggag atgattctat gcaagttaaa gttaaaaagg
    67321 ctgtaatacc agcagcaggt ttaggaacta gatttttgcc agctacaaag gcacaaccaa
    67381 aagagatgtt accaatagtt gataagccaa ctctacaata cataatagaa gaagctgtgg
    67441 cctcaggaat agaggaaata ctaataataa caggtagaaa taaaaaatct attgaagacc
    67501 actttgataa atctgtagaa ttggaacttg atttagagaa aaaaggtaag aaagaattgt
    67561 tggaggtagt acaaaacata tctaacatga ttaacataca ttatataaga caaaaagagc
    67621 caaaagggtt gggagatgca atttattgtg cgaggcattt tataggtgat gagccgtttg
    67681 cagtaatgtt aggggatgat attgtagata atgatgtacc ttgtctaaaa caactaacag
    67741 atgcatatga ggaatataga actacaatat tgggtgttca aaaggtaaat caagaagata
    67801 ctaataaata tgggataata gaagcaaaaa atatagaagg tagagtttac aaggtaaaag
    67861 acatggtaga aaagccagag tcaggaaaag ctccttcaaa tattgctatt ttaggtagat
    67921 atataataac tcctgaaatc tttgatattc ttaaggattt accaccaggt aaaggtggag
    67981 aggtacaact tacagatgct ttaaaaatat tatctaagaa agaagctatg tatgcataca
    68041 attttgaagg caagagatat gatgttgggg acaagctagg atttttagaa gctactgttg
    68101 actttgctct aaagaaagaa gaccttaagg aagattttat aaaatatttg aaacatgtat
    68161 gtagtgaatt tgataaaaat aacaatgtgt tatacaataa tacagatgaa aaaatagatt
    68221 taattgaagt caatgaagct agagaacttg aaattcaaaa ataattaatg aaaggtgatg
    68281 tatggtggca aaatcaagtc agaaaaaaat taatagaata atagctgttg ggattgtggg
    68341 aatcctcgta ataacgagta taacagctgt tttgagtatg attgcaacta tgtaataaga
    68401 taaatgacag cagttgatat acataaatat aaaaaccata tataacttga tgtagtcaaa
    68461 aacttacaat attttagtta tatatggttt ttagaattta taaatgtaaa aaaaaatttt
    68521 tataatagtt gcatatttta aaggaaaata ttattataag atataaatat agtgataaat
    68581 ttataaaaac gtgctaagat tggacttgaa atacagtaat atagtaggta ataatgtttt
    68641 aaattttatt tattgaccat aatatataaa taatgatatt attagactta actgtttaaa
    68701 taaaactttt ctttttcatg ccttcaattt ccaaaataag tatgtatata aatgaatata
    68761 tagatgagga ggattttttt gattttagat gataaagtat tagaaggatt aggtagtaag
    68821 accttagttg agctacgaga gatagctaaa gaattaaaaa taaaatcaat tactacatat
    68881 aagaaaaatg agttaattga gattataaat tccaaaagta aaaatgagga aaaagtaaac
    68941 gaagagacta aagaaaaaaa tgaagatatt aaagaagaca taaagaatag aaaagaaaat
    69001 atagagcgtg aaagtataga aaaagaagtt gaatataaga gccctaccaa atcatacaac
    69061 aagaaagaaa tagataccca aaatatttcc caaaaccaag aacatagaaa tcaaagaaat
    69121 gactataata agacaacgag tgtaaatgat agcaataagt catcaaatat atctaataga
    69181 atggtcagaa ataataataa aaactattat atgcctaaac aagttgatga atctaagata
    69241 gtagatgaat ttaacacatc aaaagaggat gaagtagtag gtgtacttga aattctgcca
    69301 gatggatttg gttttttaag aggttcaaat tatttgtcta ctgaaggaga tgtttacgtt
    69361 tcaccatctc aaataagaag atttaatatg aaaacaggag acaagattaa gggaataact
    69421 agacatccaa aaagtggaga gaaatttaga gcacttttat atgttcaaaa aataaatgat
    69481 gaaaaccctg acacagctat acaaagaaat gcgtttgaga cattaacacc aatatttcca
    69541 gaagaacgac ttacactgga aactaataga aatgaaatag ctacaagaat tatagattta
    69601 atttcaccta taggaaaagg acaaagagga ttgatagtag cacctccaaa agctgggaaa
    69661 acagttcttt taaaaagcgt tgcaaatagc atagctaaaa atcatccaaa tgttgaactt
    69721 atagtactcc ttatagatga aagaccagag gaagttactg atatgaaaga atctattgaa
    69781 ggagacgtta tatattccac ttttgaccaa gtatcaagtc atcatgtcaa ggttgcagag
    69841 atggtgttaa atagagctca aagattagtt gaacatggta aagatgtagt aattttacta
    69901 gatagtataa ctagattggc aagggcatat aaccttacaa tatctcctac aggtagaact
    69961 ttatctggag gtattgaccc tggtgcactt catggaccta aaaagttctt tggtgcagca
    70021 aggaatatta gacagggtgg ttctttgaca atattaggaa cagcattggt tgaaactggt
    70081 tctaggatgg atgatgtaat atttgaggaa tttaaaggta caggaaatat ggagctacat
    70141 ttagatagaa aattagcaga aaaaagaata ttccctgcaa tagatattta caagtctggt
    70201 acaagaagag atgatttatt gcttgatgat gaagaaaaga ctgctctttg gagattaaga
    70261 agagaaatga gtaataattc agtaatggaa attacagata aagtcattga acttataaag
    70321 agaactaagg acaataaaga gtttgtaaaa tcaataaaga atttgtaaaa tcaataatac
    70381 aaatgcaaat atggattata taaactaata gaaaaatcac ttgaatggca taataagttg
    70441 tgctataata atgtagttga taatgtaaaa agaaagttaa tataattaat ttttttaaat
    70501 aaagagaaga ggtgactata atgcaaaagg aaatacaacc aaaatacaat ccagttgaag
    70561 tgcgttgtgc ttgtgggaac acatttgttg ctggttcaac taaggacgaa ataaaagttg
    70621 aaatatgttc agagtgccat ccattctata caggaaaaca aaagaacata gaaaaaggtg
    70681 gaagaatcga caaattcaag aaaagattca aaatggacta atttttataa aagtggagtt
    70741 ggaggaagcc aactcctatt ttattacaaa aaaacattca tattctactt taaattgtct
    70801 ttttgtatta taataataaa tatgtaaaat tgagtattct aaatacaata aaatgaggtg
    70861 agtggatggc aaaacaagct gtaggtgggc aagctgttat tgaaggtgta atgatgcagt
    70921 caaaagacaa aagagcggta gctgttagaa aaagtgacgg agaaatagca ctaaaagaag
    70981 atagaataaa aagttgggta agagataaga atatagataa gatacctttt gttagaggtt
    71041 cttttgttat gatagataca atgattcaag gcataaaaag tttaaacttt tcttcagagt
    71101 tttttatgga agaagctgaa gaggataaat ttgatttatt tataaagaaa atttttaaag
    71161 ataaggcaaa tgatataatt ataatatttt cattggtcat agcaatgtta ctttctgctg
    71221 gattattcat ttttatacct acattggtag gaggagcttt ttctaaagta atgcctaatg
    71281 attttatgct taatttaata gaaggaatca taaggatagc tatattattt gcatatatag
    71341 tattgatttc acgaagtaag gatatagaaa gagtctttca atatcatgga gcagaacata
    71401 aatctatata ttgctatgaa aatgatttag aacttacagt agagaatgct agaaaattta
    71461 aaagattaca tcctagatgt ggtacaaatt tcctatttat agttatggct gtatcaataa
    71521 tattgtttgc attttttggt tggccaaatc caattcttag aatatttatg agaattatat
    71581 gtgtgcctat agtagcaggt ttatcttatg aggtaattag agttcttggg aaatatgata
    71641 atggatttac taaaattatt gcatatccag gaatgatgtt gcaatatttt acaacaaaag
    71701 agcctgatga tgagcaatta gaggttgctt tggaagcatt gaaagcagta gtagattaga
    71761 gaaaaggata tttgttatga cgataaaaga tataattatt aaatattcag ataaactaaa
    71821 agatataagt gatactccaa gattagatac agagttattg ttacaaaaaa cattgggtgt
    71881 tgatagatta tatattcact taaatctgaa taaagagcta actgaagaac aaaaaacaaa
    71941 atttatgggt tttgcagaag aaagattaaa tggaagacca atagcatata tagttgaaaa
    72001 tagggagttt atgggattag atttttttgt taaggaaggc gtattgattc caagaccaga
    72061 tacagaaact ttagtagaag aaataattga aatatgtaga gaaaaaaaag atgtaagtat
    72121 attagatata ggaactggtt ctggagctat tacaataagt ttagcaaagt acattgaaaa
    72181 ttctaaaatc atgtcttttg atatatcaga gacagcatta gaaatagcta aaaaaaatgc
    72241 tattataaat gaagttggtg aaaaaataaa gtatattaat tctgatttat ttacagcaat
    72301 aagtgatagt aacataaaat tcgatatcat agtttcaaat ccaccatata ttaaaaaaca
    72361 agatatagaa actctgcaca aacaagttaa agattatgaa ccatataatg ctcttgaagg
    72421 tggagaagat ggattagact tttatagaag aataacagaa caaggtaaga aatatttaaa
    72481 taagtgtgga atattagcat atgaagttgg tcataatcaa gctgaagatg taataaatat
    72541 tatgaaaagt aatggatata aaaaaatata cacaaagaag gatattcaag gcattgatag
    72601 agttgttata ggttacaata tatgatataa tcaacaattg atatgttaca tttaatcgag
    72661 gtgaaagtat gttaaaaaaa ttagaggtat tggaagatac atataaagac ttaagtgaaa
    72721 aaataggtga tccggatgta ataaatgatc aaaaagtttg gcagaaatat ataaaagaac
    72781 atgctgactt ggaacctatt gttatgaaat acagagagta taaaagcgtt ttagatagta
    72841 taaaagaatc aaaagaaatt ttacaagaag agtcagatga agaattaaga gaattagcta
    72901 aaatggagtt agctgagatg gaagaaaaag tagcacctct ggaggaagaa ataaaaatac
    72961 tattattgcc taaagaccct aatgatgata agaacgttat agttgaaatt agaggtggag
    73021 ctggaggaga tgaagcagct ctatttgctg gtgatttatt tagaatgtac tcaaggtatg
    73081 ctgaaagaag aagatggaaa atagaattat tgagtgcaag tgatactgga gttggtggat
    73141 ataaagaagt atcttttatg ataaaaggta aaggtgctta ttcaagatta aagtatgaat
    73201 ctggagttca tagagttcaa agaataccat caactgaatc tggtggaaga atacacacat
    73261 caacttctac tgttgcagta ttaccagaag ttgaagatgt agaggttgaa ataaatccaa
    73321 atgatttaag aatagatgtt ttccgttctt cgggtaatgg aggacaaagt gtcaatacta
    73381 ctgactctgc tgttagggta acacatatac ctacaggtga agtagtatct tgtcaagatg
    73441 gaaaatcaca attaaaaaat aaagagcaag ctttaaaaat attaaaagct agactttacg
    73501 ataaagcatt ggcagaacaa cacaaagata tagcagcaga aagaaaaagc caagtcggaa
    73561 caggagacag gtcagaaaga ataagaacat ataatttccc tcaaggtaga ataagtgacc
    73621 atagaataaa tttaacttta tataagttag atgcattctt agatggagat atagatgaaa
    73681 tgatagatgc tttaattact gttgaccaaa cagaaaaaat gactgcaata taagtaatgt
    73741 tttaaagaag ctgtttcttc ctagaaatta ttttctattt tgaaacagct attttgcatt
    73801 ttaggtcatg ttatcatatt ctattgtata taattgttat atacaagtat aggagggtta
    73861 tatgatacta aaagtaacaa taattgggct tttggcaggt gttataggga caggattagg
    73921 aggagtaata tcagccattt ttaaaagaga ggtcgataaa tatctaagtt tttttatggg
    73981 attatctggt ggaataatgt tggctgtagt ggtgtttgat ttaatgaaag aatccatgga
    74041 caaaatgggg ataatcaata cagttatatt tacgtttgtt ggagctctca taactatgta
    74101 cataaaaaca aaattagatg tgtctggaaa tatggcatct ggatatctta tattcataag
    74161 tatattacta cataatttac cagaaggact agccattggt tcttctttta tgtcaactga
    74221 aagtttaggt attacacttg ctatagttat aggtctacat aatataccag aagggttagc
    74281 tatggcatta ggcttagttt gcaataaaat gaaactaagt aaagttattt tgtttactgt
    74341 aatagctgga ttacctatgg gtctaggaag ttttttaggt gtttattttg gaggagtatt
    74401 tacttctctt ataggagtat ttcttgctac agcaggaggt actatgatgt atgttgtatt
    74461 ggaagaaatt tttccacatt caaaaagtgt gtattgtata attggtttct tgttaggtac
    74521 aatgattgta aattatatat aaaattaaaa tgaaagcagg catacaaaag atgaaaacta
    74581 ttataagtaa tattgatata aataatattg attatgagga aataaaaata caagcaaaac
    74641 ttcttagaga aggtaaaaca gttatttttc caactgagac agtatatggg ttgggtgcaa
    74701 atgcattaga tgaaaatgca gtaaagaaga tttatgaagc taaaggaaga cctagtgaca
    74761 atccattgat agtccatata tatgaaaaag aagaagtata tgacttagcc aaagatataa
    74821 gtgataaagc taaacttgtt atagaaaagc tttggccagg acctataaca attatattaa
    74881 ataaaaaaga tataattcca tacaaaacta gtggagggct tgatacagtt gcaataagga
    74941 tgccttctaa tgttattgcc agggcaataa taaaagaagc tggtatacct attgcagctc
    75001 cttctgcaaa tatctcaggt cgcccatctc caacaaaagc aaaacatgta tatgaagaga
    75061 tgaatggaag agtggatgga atagttttag gaggagacag taattttgga ttggagtcaa
    75121 ctgtattgga tttaacagaa gagactccta tgatactgag accaggaagt ataactaaag
    75181 aagttttaga ggaacttctt ggagaagtaa agcttgaccc atcattaagt aaaaaagaag
    75241 ataatcaaaa agctaaagct cctggaatga aatataaaca ttattctcca aatgcagatg
    75301 tatatataat atctggaaaa agagaaaatg tagcaacaaa gattaatgat atgataaaat
    75361 ctaatagaga caaaggtcta aagacaggtg taatgtgtat tagtagaaac cgagagtttt
    75421 atgatggaga agtaatcgat ttaggaaact ctcttgaaga agttgcaagt aatctgtttg
    75481 atgctcttat agaaatggac aaaaagggtg ttgatgtcat atattcagaa gagttcccta
    75541 agactggagt tggacaagca attatgaata gactactgaa gtcagcagga tacaaagtga
    75601 taaaggctta agagggagtt gtaaaatatg aatatactta ttgtgtgtac aggtaatact
    75661 tgtagaagtc ctatggctga ggctatacta aggaaagcaa ttaaagaatc tggaagaagt
    75721 attgaagaat attctatatc atcagcaggt atatctacag caaatggaat gggtgcttct
    75781 gaaaattcta tagaagtact aaaagaaatc ggtatagact tatcaaatca tagaagtaaa
    75841 gtaattacta aaaaattaat tgatgaatca gatataatac taacaatgac taaatctcat
    75901 aaagaaattt tagtacaagc agttccaaaa tgcaaagaaa aagtgtatac ttttaaaggg
    75961 tttgcaaata aaaatgagga agatatatct gacccatttg gtggaaattt ggatatttat
    76021 agaagcacta tgagagaaat aatgtattct gtcaacgaaa tagtaaaaaa aatatgaaac
    76081 ggaggataaa caaatgaaga taggattagg ctgtgaccat ggaggatata atttaaaaaa
    76141 agaaataata agctatttag aaagtaaagg tatagaatgt gtagattacg gaactaataa
    76201 tgctacagat tctgttgatt atcctgttta tggagaaata gttgcaaatt cagttataaa
    76261 taaagaagta gattatggaa tattatgttg tggtacaggg ataggaatat ctttagctgc
    76321 taataaggtt cctgggataa gatgtgcagt tgtatcagat gtattttctg caaaaatgtc
    76381 aaaagcacat aatgatgcaa atatgttatc attaggcgaa agagttttag gaaaaggtct
    76441 agcacttgag atagttgaag catggattaa tacggatttt gaaggtgata gacatgctag
    76501 aagagtaaac atgattaaat caatagaaga aaaacataat aaataataaa aattacagtt
    76561 ttatcaggag gaattatcaa aatgagcaaa gtggtagaga caaaccatcc attaatacaa
    76621 cataaattaa ctttaatgag agataaaaat actggttcta aagactttag agagctttta
    76681 actgaaattg caatgttaat ggggtatgaa ataacaaaag atataccatt aaaagatgta
    76741 gaaatagaaa ctcctattca aaaaacatca tctaaagtag ttgcaggtaa aaaattagca
    76801 ataattccaa ttttaagagc aggtcttgga atggtagatg gattagtgag cttaatgcca
    76861 gcagcaaaag ttggacatgt aggactatat agagacccag aaacattaaa accagttgaa
    76921 tattattgta agcttcctca agacatagga gaaagagata taatagtagt tgaccctatg
    76981 cttgcaacag ggggttctgc tgtagcagct atagatttac ttaagtctaa aggagctaaa
    77041 agtataaaat tagctaattt agtagctgca ccagaaggta tagcagaagt gcaaaaatat
    77101 catgatgatg ttgatatata tgttgcatca gttgacgaaa gattaaatga acatggatat
    77161 ataattcctg gtcttggtga tgctggagat agattgtttg gaactaaata aaaataaata
    77221 actataaata tttattaggg actttcttat atagtgtata taattttatg taagaaggtt
    77281 cttttttata tattttttct caaaatagga attattgaat tgataaattt ctcgaattga
    77341 gaataaaaag tttttaccaa ttaaaaaaga ttgcttattt tacaaggttt tatagaatag
    77401 tagtctattt taatgtggca tgttagttgc ttacgattaa gattataggt attaagtgta
    77461 ttagttaaat tgagaattga atattaataa tattattggg agtggtaata catgaagaat
    77521 tgtatagtag taagaggggc tggagattta gcaacagcag ttattcataa attacacaac
    77581 agtggtttta aagtagttgc gttagaaata gaagagccat tagctataag aaggcaaatt
    77641 tctttttgtg aggctgttta tgaaaaaaaa gtaactgttg aaggtgtaga atgtatattt
    77701 tgcaatactg aaaaagaaat agaagaagtt ttatctcaac ataaagtagc cttgattata
    77761 gacccaaaag gagaaaaatt atctaaatta aatccaatcg ttttggttga tggaatactc
    77821 gctaagaaaa acttaggtac aactaaagat atggcaccac ttacaatagc tttaggtcct
    77881 ggattttgtg caggagaaga tgtagatgtt gttatagaga caatgagagg tcataatcta
    77941 ggaagaataa taactaatgg tagagcagaa aaaaatacag gagtaccagg agcaataaaa
    78001 ggtttttcta aagagagagt tatgcattca cagtatgatg gcataatgga aaatgtatgt
    78061 aagataggtg atatagttga aaagggagac ctactttgct atattaataa ttatgaaaaa
    78121 aaacatgaag ttagagcaac aatatctgga gtccttagag gtttattaaa ggatggagca
    78181 aaggtaaaca aaaatttaaa aatactagac atagacccta gaaaagaaga agttaagaat
    78241 gcttttacaa tatcagataa agctagatgc atagctggag gggttttaga agccatacta
    78301 aacaatggga tttatcccca aaattaattt atattagata aagctattaa tattttatta
    78361 agatgatata ataaatttaa tagattaaag agagcatatg acgggtattt aaaatttaaa
    78421 tggtaaaaat gctctttttt tattggacat tcaattaaca tgtgtatata atataaaaat
    78481 aagtatttat ttaggttgaa atctaataaa tatataatac cagtacataa gcaaagagga
    78541 gaaaagtaaa ttatgagacc atcatgggat gaatacttca tggaaattgc agaagtagtg
    78601 aaaaagcgtt caacttgtat tagaagacaa gtaggagctg taattgtaag agacaaacaa
    78661 atattaacaa ctggatataa tggttctccg agaaatcttg aacactgtga aaatattgga
    78721 tgtaagaggc aggaattaaa tattccctca ggagaaaggc atgaattatg cagagcatta
    78781 catgctgaac aaaatgcgat aatacaagct gctcataatg gtattagtgt agatggggca
    78841 actttgtatg ttactacaag accttgtgtt ttgtgtgcaa aaatgtgcat aaatgcaggt
    78901 atattgaaaa ttgtttatga aggtgattat ccagatgata tgtctacaga attattaaaa
    78961 gaagcaggaa tagaactcat aaaattttag tggataaaat ttataagaca ataaatgtca
    79021 aattatgaca ctgaaataca atggtatcaa atgattaaaa gaaagtattt gaaaaatcat
    79081 cagattagta gtaagatata ttttataatt aaataaattt atgagataga ttaattacat
    79141 gttatttatt ttataataat gagggatact atttatgagt aaaagaaata cacatacaga
    79201 aattgcaaag ttgtttagtt taataagtca aataggattg atgattgtaa tatcaatatt
    79261 aggatgtact ttcttaggaa aatttttaga tagtaaattg aatactactc cagttctgac
    79321 aataatattt ttattgttgg gtgttggagg tgcttttagt ggtgtttata aaactctaat
    79381 agtatataca cgaaggaagt gatatattgg atataaaatt acttaaccag gtaaggtcag
    79441 taacaaaagg tatagtaata tttgatgtag ttttaattgt gctaatgttg ataacatcaa
    79501 cattaagtaa accagtatta ttaggactta tatttggctc tataatagct atgttaaatt
    79561 ttagattatt ggcgatatca ctggaaaaat cagtgacatt tcctgctggg aaggcacaag
    79621 cttatgcatc agcacaatat gctgttagat tatttataat ggcagttgtt ctaattgcat
    79681 cagctacagt accacattta aatataatcg gaacagcatt gggattattg agcacaaagt
    79741 ttgtcatatt atcaaaaaac tttataaata aattaaagag gaaggaggca taagtatgaa
    79801 tcaagcgcat tattatctca taggaaactt taagattagt gaaacaatac tagtaagttg
    79861 gggaataatg gcctttttag cgatagcatc ttattttttg actagaaact taaaaaaagt
    79921 tccaactagt aaagttcaaa tattcttaga atttgcagta ggtggtcttg ctaaaatggt
    79981 tacagatgtt atgggtaaaa atacagtaaa aagaatgcca tatatgatac catacatagg
    80041 aagcttgttt ttatttttcg catgttcaaa tttaagccca ttacttggat taaggtcacc
    80101 aacaaccgac ttagacacaa ctgctgcatg ggcaatgatt acatttttta tgatttattt
    80161 tgcaggagtt aaatttaagg ggttatcata ctttaaagaa ttggctcaac cacttgccat
    80221 aatgttacca atcaatatta taggtgagtt agctagacca atatcattaa gtttccgtcc
    80281 atttgggaat attttaggtg gaacaataat aatgggtctg ttttatcaac ttttaggttt
    80341 tatatctagt ttaataccag gattatcaat accaattgga gaaatattaa taccagtccc
    80401 attacacttg tactttgact tatttgcagg attgttacaa gcatatatat ttataatgtt
    80461 aacaatggta tttatatcaa gtgcaggaga gttagattaa gaaatcaact tatattaaga
    80521 taaaaataaa ataatttaaa aaattaaagt tttaaggagg aaataattat ggaaacagca
    80581 atagtagcgg cagcatcagc aataggagca gggatagcag ttgcaacagg tataggtgca
    80641 ggtataggtc aaggtatagc agcagctaaa gctgcagaag cagtaggaaa ccagccagaa
    80701 gctaaaggtg atataacatc aactttatta ctaggagttg ctatagcaga gtcttcagca
    80761 atatatggtt tggttatatc aataatactt ctattcgtaa atccattctt taaatactta
    80821 ggaatgtaga tttagtcctt aaatattttg agataggttt ataggtaaat catctcaaac
    80881 ttataatcat gagatgaact ttatcatctc gtgattataa gtagttatga tttatggaaa
    80941 ggaggataat tatggaaaaa gcactagtag gtataacgtg ggaatttgtg ttccaaattg
    81001 ttaatacttt tataatattt ttacttctga gaaagctttt attcaagcca gtattaaata
    81061 taattgaatc aagagaaaat gatattaaaa gtgatttggc agaaggcgaa aaagcaaaaa
    81121 atgaaggttt agctctgaaa aaagaatatg agtcaaaaat caattttgcc aaagatgaag
    81181 gtcaagaaat cataaaacaa gcaactataa gagcagagca gaaatcagat gatatagtaa
    81241 atactgcaaa gaaagatgct cttgatataa aagaaaaagc aaacaaagat atagaacaag
    81301 aaagacaaaa ggttataaat gaaatcaaaa atgatatatc taacatagca ctacttgcag
    81361 catctaaagt tattgaaaaa gatttagata aatctaaaca tgaagaattg atagaaaact
    81421 ttataaaaga ggtaggggaa gctaaatgat aaatgtaata gcaaatagat atgccgaagc
    81481 cctatttcaa ttaggagaag aagaaaattc aactgatgtc ttattcaaag aactagaaaa
    81541 agtagtagat atgatgacta aagttagtaa agatttctac aaagttttaa aatcaccatt
    81601 agtttcaaaa tcagaaaaga aaaacttggt agaaattatt tttagtaagg aagtaagttc
    81661 taacataaaa aactttttaa aagttttagt agataaagat agaatatcat atttagaaga
    81721 tatcgaatta gcttataaag aattattaaa taagaaaaac aatgtaattg atggagtagc
    81781 aataagtgct attccaatga gtgaaacaga tattaaagaa cttgaagtca aactttcaaa
    81841 taaatacaac aaaaatgtta ctatagaaaa tgttgttgac aagactatac tagggggagt
    81901 tctagttaga attggaaatg aacaaataga tggcactgtg aaaactcgtc ttgataagat
    81961 gaaagaaaag ctatctgaag taatatctta agaatggcgg tgaacccatg aacttaaaac
    82021 ctgaagaaat aagttctata attaaacagc aaataaaaaa ttatgagaat aaagttgagt
    82081 tgacagatac aggtagtgtt ttaacagttg gggatggtat agcgagtgta tatggattag
    82141 aaaaagctat gtctggtgag ttgctagagt tcccaggtga aatatatggt atggcactta
    82201 acttggaaga agaagtagtt ggagctgtta tccttggaga tgacagtgaa ataaaagaag
    82261 gcgatatagt taagagaact ggaagaatag ttgaagttcc agttggagaa gctttaatag
    82321 gtagagttgt aaactcatta ggtcagccaa tagatggaaa aggacctata gcgtacacaa
    82381 aaactagacc agttgaatct gaggcacctg gtataataga tagaaggtct gtttatgaac
    82441 cattacaaac aggaatcaaa tcaatagatt caatgatacc aattggtaga ggtcaaagag
    82501 agttaataat aggtgacaga caaactggta aaacatctat agttattgac actatactaa
    82561 atcaaaaagg aaaagatgtt atctgtatat atgtagcaat tggacaaaaa cgttctacta
    82621 tagctcaatt agttagctct ttagaaaaag gtggagcttt ggattataca atagtagttt
    82681 cagcaacagc atctgaatca gcaccacttc aatatatagc accatatgct ggagcagcta
    82741 tgggtgaaga atttatgtac aatgggaaac atgtacttat agtgtatgat gatttaagta
    82801 aacaagctgt tgcatataga gaaatgtcat tgttacttag aagaccacca ggacgtgagg
    82861 catatcctgg agatgtattc tatttacatt caagattact tgaaagagca gctaagttat
    82921 ctgatgaatt gggtggaggt tcaatgactg ccttgcctat aatagaaact caagctggtg
    82981 acgtttctgc atatatacca acaaatgtta tatctataac agatggtcaa atatacttac
    83041 aaccagagtt attctattca ggtgtaagac cagcagttga ccctggtata tcagtatcaa
    83101 gggttggtgg ttctgcgcaa attaaagcaa tgaaaaaagt tgcaggaaca ttaaaacttg
    83161 catattcaca atatagagaa cttgcagcat tctcacagtt tggttctgac ttagatgaag
    83221 atactaaaaa gagacttgct caaggtgaaa gaatcgttga gatattaaaa caaggtgagc
    83281 atcaaccaat aaaagttgaa aatcaagtta tgataatata tgctgttata aataatcatt
    83341 tagaggatat tccgatagat aatatagcta gatttgaatc agaattgtat gcatttgtag
    83401 ataataatta tccggaaata tcaagaaaaa tattaggtgg agaagacttc actcatgatt
    83461 taacagatgc aataaatgaa tttaaagaaa agtttgttgt tgaagtataa tcctttttaa
    83521 aaaaggaggt aaattaattg gcaggagctg gaatcaaagc aatcaaaaca cgtatgggaa
    83581 ctgttgaaag tacaaagcaa ataactaaag caatggaatt agttgcatct tctaagctta
    83641 gaaaagcaaa agaaaaggct gaagattcaa gagaatattt taatacttta tacaatacag
    83701 taaaagatat tgcccaaaca acacgtggaa ttagaaatga atttttaaaa gaaagagaag
    83761 taaaaaataa atgctatgta gtagtagctg gagacagagg acttgcaggt ggatacaatt
    83821 ctaatgtatt aaaggaatct gtctctcata tgaaaggtaa aaaggaaaca gtgattactg
    83881 ttggaaagaa agcacatgaa tttttttcta aaagaaacta caatatagca aaagatataa
    83941 atagtgttga agcttgtgga tatgaagatg cgacagaaat ttcacaaact attattgacc
    84001 tgtataaaaa aggtgaagta gatgaagttt atattgctta tacaaaattt aaatctgctt
    84061 tatcacaaga agttgaaatt ttgaaattgc ttccattagt ctttgaaaaa gatacagacg
    84121 aacaaggaat ggcagtaaaa gaagaaaaac aaaatagatc aaaagtacaa tatataccat
    84181 cagcagaagc agttttaagt tatattgtac ctcagtttat atcaggtaca gtatatggtg
    84241 gaattttgga atcttttgca tcagagcaag gtgctagaag aactgcaatg gaatcagcca
    84301 cagacaatgc gaatgaaatg ctatctaatt tagagttaag ttataacaga gcaagacagt
    84361 cagctgtaac tcaagaaata acagagatag ttggtggagt tgaagctcta aaatagggag
    84421 gttaggaaat ggcaaatgta ggtaaagtag tacagattgt aggagctgta ctagatgtta
    84481 agtttgatgg tgaacaaagc ctacctaact tattaaatgc gttagtaata aagttaggag
    84541 ataaagaaat agttgcagaa gttgctcaac atataggtga tgatactgta agatgtatag
    84601 ctatgagtgc aacagacgga cttgtaagag gtatggaagt tgtagataca ggagggccaa
    84661 taagtgttcc tgttggagat gagactttag gtagaatatt caatgttctt ggaaaaccag
    84721 ttgatgggaa accagctcca aaatctgcac ctaaattacc tatacataga ccagcaccgg
    84781 cgtatgatga actagaaact acagcagaga tactagaaac tggtattaag gtagttgact
    84841 tactagctcc atatctaaaa ggtggaaaaa taggtctatt tggaggagca ggagtaggaa
    84901 agacagttct aatacaagag cttataaaca atattgctaa gcaacatggt ggtatttctg
    84961 tattttcagg agtaggagaa agaacaagag aaggtaacga cctttatggc gaaatgagtg
    85021 agtctggagt tataaataaa acagctctag tatttggtca aatgaatgaa ccacctggag
    85081 cgagaatgag agttgcttta actggactta caatggcaga acattttaga gatgagcaag
    85141 gacaagacgt tttacttttc gttgataata tattccgttt cacacaagct ggttcagaag
    85201 tttcagcact tctaggacgt atgccatcag ctgttggtta tcagccaaca ttagctactg
    85261 aaatgggtgc acttcaagag agaataacat caactaagaa aggttcaata acatctgttc
    85321 aagcagtata tgtaccagca gatgacttaa ctgacccagc accagctaca acattctctc
    85381 acttagatgc aaaaacagtt ttatcaagac aaatatcatc actgggtata tatccagcag
    85441 ttgaccctct tgaatctaca tcaaggattc ttgaccctag tatagtagga aaagaacatt
    85501 atgaagtagc tagaggtgta caatcaatac ttcaaagata taaagaactt caagatataa
    85561 ttgctatact tggtatggat gaattatctg atgaagataa gttaatagtt gcacgtgcta
    85621 gaaagataca aagattctta tctcaatcat tcacagttgc tgagcaattt acaggaaatc
    85681 caggtcaata tgtacctgtt aaagagactg taagaggatt taaagaaatt cttgaaggga
    85741 aacatgatga cttaccagag tcagcattct tatttgtagg aacaatagaa gacgctgtta
    85801 gaaaagcgaa gggaagtatg tagtcatggc cagtgaattt tcattgaaag ttgtaactcc
    85861 aagtaatact ttttatgatg gagatgttga aatgatagta gttagaacta caaaaggaga
    85921 caaaggggtg ttgaaaggtc atataccatt tgtttcaggt atacctgtag gaactttaaa
    85981 gataaaaaaa gagggaagtt ttaaagaggc gaaaatagca gaaggattca ttaatgtaac
    86041 tcaagataaa actgtcatat taacagaatc tgctgagtgg atttaataaa ttaatttaga
    86101 gtataaaaag tcattttgac caaaaaaact tttgggtaaa atggcttttt tgtgttttgg
    86161 tgttaataaa ataacgataa aattgtaagt ttttagtgat aataaattca caaaatgttt
    86221 aaatttactg ccaaattatg ttgtaattaa taagataaag aagatttaga tagcttttat
    86281 gtaagtgaaa aagtcttcat ataagaaata catataaaat ttttataaaa tttaaaaaat
    86341 ttgcttagtt gtgtagtata ataaataata taatagattt gtatataata ttcattacaa
    86401 ataattttaa aagttacagt tttattataa tgattattaa attaagataa gtaacataaa
    86461 ctaaataata atgtgtatta tcgatagtaa atttataaat tcattattaa ttattgaaag
    86521 aagtattata aattttctgg aggaacaatg aatatatttg atattggcgt agatataatt
    86581 gaaattgata gaataagaaa ggctgtagac aaaaataata ggtttttgga gaagatattt
    86641 acagatagag aaatagagta ttttaattct aagaatttta aagcagaaag tatagcaggt
    86701 aatttcgcag ccaaggaagc tataagtaaa tctataggta caggaattag attgtttaat
    86761 tttaaagata tagaagttct cagaaatgaa atgggaaaac caatagtaaa aacttataat
    86821 aatctagcaa aaatgtgcat agattataat gttttagaaa taaaagtttc aatatcacat
    86881 tcaaaagatt atgctatagc aaatgcgata accataatta aggactaagg agtgattaca
    86941 ttatgatgga taaaacagca aaagaaataa tgacaacaga tgtaatagta gcaaaacaag
    87001 atgatagtat agcagatgta gctaatatgc taattgcaga aaaaatagga ggtctgccag
    87061 ttgtagattc agagaataga gttgttggaa taatttctga aacagatatt cttaaaaaag
    87121 aaaaatatat agaagcacct ctctatataa atctattaca aggattgatt ttcttagatg
    87181 acttaaaaaa agttgaaaaa gatataaaac aagtagctgc ttataaggtt ggagaactta
    87241 tgtctaaaga tataataaag gttcatgaag atgataagtt tgatgatgta gctaatgtaa
    87301 tgattaaaaa atcaataaac agagttcctg tagtagatga tgacaacaaa cttaaaggta
    87361 taatttgtag atatgatata ataaaagctt tatacaatga ataaactgaa gtaggaggtg
    87421 tgatgtgaga aaaaagtgga ccatagtatg tattatgttc ttggcactgg ttataattgt
    87481 aataggatgc caaaaacgac agtccacaaa agaagaagta tacaaagatt ttcaaaagca
    87541 gatttctgat atgaattatt acagttgtaa agctgaagta gaagtagttg gaaataaaag
    87601 tccacacaat tatgttttaa ttcatactta taaaaaaacc gataactata aattagaagt
    87661 aatttcccca aaacatttaa aagggaaaag tatagaatat caaggtgata aaatattagt
    87721 taaaaatcca aagattagtg atgtagtgga gttacctaat acaggaaaaa ataatcaata
    87781 tctttttgta ggagacttta taaaaaacta tcttcaaaat gaggagatga aagtaaagtt
    87841 atccaaagga catttggttt tagagacttt tattcctggg gataataaat attttaacaa
    87901 gcaagtatta tatgtaaatg ctgacacaaa aaatcctgaa aaaatggaag tgttagataa
    87961 agagggagtg ccaagattta cagtaaaata caaagatttt gaatacagaa actaaggggg
    88021 actaaagaca tgcaaaaaat aacagtgcct acatgggcag agataaatct agataactta
    88081 agatttaact taaataatat taaaaattta ttagaagaag atattaagat ttgtggagta
    88141 ataaaagctg atgcatatgg acatggtgca gtagaagttg caaaattgct agaaaaagaa
    88201 aaagtagatt acttagcagt agcaagaact gctgaaggaa ttgaacttag acaaaatggc
    88261 ataacacttc ctattttgaa cttgggatat actccagacg aagcttttga agattctata
    88321 aaaaataaaa taactatgac agtttattct ttagaaacag cacaaaagat aaatgaaatt
    88381 gcaaaatctt taggagaaaa agcctgtgtt catgttaaaa tagactcagg gatgactaga
    88441 ataggtttcc aacctaatga ggagtcagta caggaaataa tagaattaaa taaattagaa
    88501 tatatagatt tagaaggtat gtttactcat tttgctacag ctgatgaagt aagtaaagag
    88561 tacacttata aacaagctaa taattataaa tttatgtctg ataaattaga tgaggctggt
    88621 gtaaaaatag ctataaaaca tgtatcaaac agtgcagcta ttatggattg ccctgattta
    88681 agattaaata tggtaagagc aggaataata ttatatggtc attatccatc tgatgatgta
    88741 tttaaagata gattagaatt aagaccagcc atgaaattaa aatcaaaaat cggacatata
    88801 aaacaagttg aaccaggtgt aggaataagt tatggactaa aatacacaac tacaggtaaa
    88861 gaaacaatag ctacagttcc aataggatac gcagatggat ttactagaat ccaaaaaaat
    88921 ccaaaggttc ttattaaggg agaagtgttt gatgtagttg gtagaatatg tatggatcaa
    88981 ataatggtta gaattgacaa agatatagac ataaaagttg gagatgaggt tatactattt
    89041 ggagaaggcg aagttacagc tgagcgtata gctaaagact taggaactat aaactatgaa
    89101 gtgttatgta tgatatcaag aagagttgac cgtgtttata tggaaaataa tgagcttgta
    89161 caaataaaca gttatttgct aaaataaaac catgatatta taatctggga ggcgattttg
    89221 tgtacgataa gagtaaagaa aaaatagaag ttactttacc tgactctctg atttctgaag
    89281 ttgatagtat agttcagatg gagaacagta acagagaaga ttttgcaaaa gctgcttttc
    89341 agttctatat aactcagaaa aagaaaattc atattaaaga atctatgaaa acgggttata
    89401 gagaaatggg tcaaattaat ctgtcactag ctgaattagg tatgacagtt gaagaagtat
    89461 cttctgatga taaaaatgaa gggaagattg cccaaggtga gtactaattg tgaaataaag
    89521 cgtggtgatt tatattacgc tgatttaagt ccagttgttg gttcagagca gggaggcgtt
    89581 agacctgttc tgataataca aaatgatgta ggtaataaat atagtcctac agttatagta
    89641 tcagctataa catctcagat taataaagcc aaattaccta ctcatgtaga gataagttcc
    89701 aatgaatatg ggcttaataa agactcagta attcttttag agcaaattag aactatcgat
    89761 aaaaagagac tgagggaaaa aattggttgt ctagatgaaa atatgatgat aaaagtagat
    89821 gatggacttc aaataagttt aggattgttt actttttaaa gagggatttg tcttttatta
    89881 gacaatccct tttttctttt aaatatctct taaggtttaa ttttaatgta ctatgttaat
    89941 aaaattaaaa ataacgcaaa aaaatagcat aaaagtgata taataatatg gaattgatat
    90001 aaaattacga actcaaggga ggtaattgca tgagagacca taaaggatta aatgagattg
    90061 cgcgtatcat aagaagagat atagtttcaa tgatacatag agccaaatct ggacatccag
    90121 gagggtcatt atcagtagtt gaaatactta ctgcgttata ctttgatgaa atgaatgtgg
    90181 attcttcaaa tcctaaaatg gaggatagag atagatttgt attatcaaaa ggacatgctg
    90241 caccagcatt atatgctaca ttggctgaaa aaggttattt tgataaggaa gaactgaatg
    90301 gattaaggaa gataggaagg atgttacaag gtcatccaga catgaaaggc acgccaggtg
    90361 tggaaatatc tactggttct ttaggtcaag gtttttcagt tgcatgtggt atggctatgg
    90421 catcaaagtt ggataatgct ccgtggaatg tgtatacttt gcttggtgat ggtgaggttc
    90481 aagaaggaat tgtttgggaa gctgctatga gtgctgcaca ttataaactt gataatttaa
    90541 ttgctttttt agataataat ggacttcaaa tagatggaga tatagaaagc gtaatgagtt
    90601 taggttcaat agttgataaa tttaaagctt ttggttggaa tgtaattgag atagatggac
    90661 atgattttga ccaaatattt gcggctcttg atatagctaa atcaacagtt aaaaaaccta
    90721 caatgatagt tgcaaaaact ataaaaggca aaggaatatc ttttatggaa aatcaagcag
    90781 gatggcatgg gacagctcca agtgatgagg agctagaaaa agccttgctt gaattaggag
    90841 gtgctgacaa tgagtaaaat ggcgacgaga gaagcatatg gtaaagcatt agtaaaatta
    90901 ggtcaaataa atgacaatgt tgtagtatta gatgcagatt tatcaaagtc tacaaaaaca
    90961 catgattttt acaagtcatt tccagataga ttttttaata tgggtatagc tgaacaaaat
    91021 ttaataggcg ctgcttgtgg attatcaact gctggtaaaa taccttttgc aagtactttt
    91081 gctatgtttg ctacgggaag ggcttttgag attataagaa attcagtttg ttatccaaag
    91141 ctaaatgtaa aaatatgtgc tactcatgca ggtcttactg ttggagagga tggagcatct
    91201 catgaaagtg tggaagatat tgctataatg agagctatac caaatatgac agttcttgtt
    91261 ccagctgatg gagttgaaac agaaaaaatt atttttgaaa tagctaagta caatggacct
    91321 gtttatgtta gattagggag aagttcagta ccagttttat ttgatgaaga ttataaattt
    91381 gaaataggaa aaggtacagt cttaagagaa ggaaaggatg tatcaattat agcttgtgga
    91441 attatggtaa atgaagctct attagcacaa gaaaagttgc aagaagaagg tataagtgct
    91501 agagttataa acatgtcctc gataaaacct atagacaaag atttaatatt agaatcagca
    91561 aaagaaacga atgctattgt aactgtagaa gaacatagca taattggggg attaggttct
    91621 gctgtaagtg aggtagtagg agagtcttgt ccaaccattg taaagaaagt tggaattaaa
    91681 gatacatttg gagaatctgg tactccaaat gaattgttaa aaaaatatga attaacttgt
    91741 gatgacataa taaagactgt taaagaagtt attattgcaa aaagaatgta agagatataa
    91801 attattcatg tttgttaata atataatata ttt
//
LOCUS       DAGSRM010000005        90542 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.005, whole genome shotgun sequence.
ACCESSION   DAGSRM010000005 DAGSRM010000000
VERSION     DAGSRM010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 90542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 90542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..90542
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.005"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            188..904
                     /locus_tag="MJE33_000381"
     CDS             188..904
                     /locus_tag="MJE33_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY9646361.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMKLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVSIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAVNTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
     gene            879..3212
                     /locus_tag="MJE33_000382"
     CDS             879..3212
                     /locus_tag="MJE33_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBY9646362.1"
                     /translation="MDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQASAKGGA
                     ENPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVLD
                     TTKNDASKENSDENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSYG
                     VNQKIINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKVISKGDSVEYGY
                     GNTYFDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLS
                     RIPLEILFIALDIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAKMASILVSLMNLT
                     YWFVIFSIIFVFIILLKHILNVGIREYFTKHTVTGMFFVWLKKAGKTVINQIRTINLK
                     EKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQLL
                     DVTKKISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNVS
                     HDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSGD
                     VYLNIVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENLI
                     INISKYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTEG
                     SGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
     gene            complement(3529..6207)
                     /gene="mngB"
                     /locus_tag="MJE33_000383"
     CDS             complement(3529..6207)
                     /gene="mngB"
                     /locus_tag="MJE33_000383"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBY9646363.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDKYRIRDLVQRRRLVIGPWYTQTDQLVISGESIIR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDIIVKLKVKASTNNVYFPNG
                     FDQAPIRKNLPDILKKANEIDNENEYEISDIENYIDSVRNERNEFTELKGEFLNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLVIKDIWKLMFENA
                     AHDSIGSCNSDTTNEDVYTRYKQARDLSINLLDLHMRLISTQIKNNNEEITLTVFNGF
                     PEVRNEVVEFDTYIPGQDFVIKNKNGKILNYIIKEKEDITNYILTQTIRLNPSKKIYI
                     PKTVYRAKIALSVKDIPPMGYTQLVFELDTKNKCEIETSNCKEIENKYYKITANKNGS
                     LEILDKESNKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNIQEE
                     MILKYSLTIPASLEERAIGKVSVKMPVTMKITLEPKEQIIKFNVNIKNNQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTVLEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQRGIALITGCVREYQIVGDNLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFDFGLYIYNNKFDEANVANTAKRFLTPIQIYEYADFLNGRLIFAFNDEKQ
                     IYENEFSLMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDAKGSLNINKNVNDAYS
                     AMLDEIYDNTSKLKVNTKTVEVNSLSHCKVQTIVIK"
     gene            complement(6282..7388)
                     /locus_tag="MJE33_000384"
     CDS             complement(6282..7388)
                     /locus_tag="MJE33_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBY9646364.1"
                     /translation="MSKKNKSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAV
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPAMGAGFIGGL
                     IASNTNAGFLGAVIAGFFAGYVCKFLKKKIKISGSASGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGAMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSKFSNALAETGKGNIVMGIIGITEGAI
                     PFTLTNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISIGSGWAYLVGAIF
                     GALVIAILSTTLVDFNEDETTENTEFDEIELDIM"
     gene            complement(7502..7810)
                     /locus_tag="MJE33_000385"
     CDS             complement(7502..7810)
                     /locus_tag="MJE33_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBY9646365.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHEIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGIALKKPEAVLTKCIEAIS
                     "
     gene            complement(7813..8265)
                     /locus_tag="MJE33_000386"
     CDS             complement(7813..8265)
                     /locus_tag="MJE33_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY9646366.1"
                     /translation="MVLQEKYIFIDKDLKSKEEVLNFIADSAYILGITTNKEALLKDL
                     LKRENEFPTGIKDGFAIPHTKSVNVKEASILYIKTTNELEWKTLDDSKVRYVFSLLAP
                     HKNEGNIHLKTLSKLATCLMEDDFTSEVKNASDASHLVKYITNKMKEE"
     gene            8499..10427
                     /locus_tag="MJE33_000387"
     CDS             8499..10427
                     /locus_tag="MJE33_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBY9646367.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKTLSNHLKVSERTIRNDITSING
                     TLEKNGAIIKIKKGEGYYIDILNLALYQQYLALISDDIMDSSEIPDSPIERNQYILKY
                     ILYNNTYIKLEDLANSLYVSKFTILNDIKRIKPILSKYNLILVSKPYYGVKVEGKEID
                     IRRCISNNMINRNFENYIIGITDREIELFNNVDLIELQRVVLSEINKFNINFLDFNLK
                     NFIIHLAITVSRILDGYCLDNVLDVVLTDFQSNTTVENIFNYIESKYTIIISKADRVY
                     LYNHFITKSSLLDNVSNRVDTKIIEYVEEILEVINNQYTFDLRNDSVLFDDLVLHFKS
                     ILNSKSYNLNKVNPLINTIKSNYPLAFEITLNAIEKVFKNSIYSLTEDEIGYVSLHIG
                     AGIERFFQSNIKCKNVVLVCGSGYGSSRLLEVQLNKVFHDKINILQCLSFNQFLASEL
                     SDVDIIISTIPLNHDSIPIVLVDLKLLKKDIENISKSITNNSHIYSNLLDNFFDKNLF
                     IVNPKIKDKDELIKLMCNKLTQSEIVFPSFAESVFYRESLSSTNIDDFLAIPHPMELS
                     SIRTKICISILNEPIYWSENSTVKLIMMLAINKDDYIKINSIYDILLKIIHDNDIRDS
                     ISNCNDFDNFLSIIKSIV"
     gene            complement(10687..11448)
                     /locus_tag="MJE33_000388"
     CDS             complement(10687..11448)
                     /locus_tag="MJE33_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBY9646368.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKEHHNKLLAKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIREKYGKDIIDTSNKKLKNMSK
                     QDYENWQNLNIEIISKLKKAFKTGDASSELAQEVAKLHHKWLCYTWNTYSKEAHSSLA
                     QMYVYDERFTSYYDREQPGLAKFLRDAIVIYTRDK"
     gene            11667..12143
                     /locus_tag="MJE33_000389"
     CDS             11667..12143
                     /locus_tag="MJE33_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBY9646369.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETSFICEYKGKEVDIIYKVFSKSGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSRKKGILDIVESISEFSNNSSMKN"
     gene            12241..12393
                     /locus_tag="MJE33_000390"
     CDS             12241..12393
                     /locus_tag="MJE33_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HBY9646370.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(12553..12741)
                     /locus_tag="MJE33_000391"
     CDS             complement(12553..12741)
                     /locus_tag="MJE33_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBY9646371.1"
                     /translation="MAKVCSVCGKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            13102..13452
                     /locus_tag="MJE33_000392"
     CDS             13102..13452
                     /locus_tag="MJE33_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBY9646372.1"
                     /translation="MSAKVTNQYGNIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            13471..15114
                     /locus_tag="MJE33_000393"
     CDS             13471..15114
                     /locus_tag="MJE33_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBY9646373.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKELVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVENDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGEFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGDNIEIKNVDLDTNI
                     STSMDFAKSTTSTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKHINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALNSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            15195..17252
                     /gene="recG"
                     /locus_tag="MJE33_000394"
     CDS             15195..17252
                     /gene="recG"
                     /locus_tag="MJE33_000394"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBY9646374.1"
                     /translation="MDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDRN
                     NLKKIAQLEDGEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIKN
                     TFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIMS
                     IIRTVLEDKELIIQEYMPQRIIEKYRLCSIDFAVRNIHSPSSKESLKIALYRIVFEEL
                     LILQLGLFVFKSGRNKEDGIKFETSKDLKKIISALPFKLTKAQNRALDEIIQDMNLEK
                     IMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFGI
                     NVGLLIGSLTKKQKDTVLEQIKNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFGV
                     MQRSKLSLKGANPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETIAIEKS
                     KRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGLL
                     HGKMKSSEKDEVMRLFKDKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQL
                     RGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLAIRGPGEFFGTRQ
                     HGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKEI
                     SLN"
     gene            17311..17865
                     /gene="rsmD"
                     /locus_tag="MJE33_000395"
     CDS             17311..17865
                     /gene="rsmD"
                     /locus_tag="MJE33_000395"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBY9646375.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKSCTFVDISKESIDIVKSNIKKARVESESIILNLDFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            17867..18364
                     /gene="coaD"
                     /locus_tag="MJE33_000396"
     CDS             17867..18364
                     /gene="coaD"
                     /locus_tag="MJE33_000396"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBY9646376.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            18368..18892
                     /locus_tag="MJE33_000397"
     CDS             18368..18892
                     /locus_tag="MJE33_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646377.1"
                     /translation="MKIDLEMIELFEQLEEIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLREIL
                     EEVKRDKAELRPKK"
     gene            19236..21155
                     /locus_tag="MJE33_000398"
     CDS             19236..21155
                     /locus_tag="MJE33_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBY9646378.1"
                     /translation="MKERAKKILDFIIKNNNVTSQELQEKFNVSKRTIYYDILAINEQ
                     LGKSGNIKNVKHKFIFEGNLCDARKIISTEEDKFLDSDYRKTFILNKILLGEKISIEK
                     LTNEMLLSKNTVVQTITDVKKYLQTMGLRLEYKGKYKIIGDEYVIRELFLIIVQENIL
                     EINSISEEVSSFDTKGHIKLTDYSLLNLTKFVEFLNKRIRDGKTLYSYKYLNEAKKIS
                     YFSNCKELLCEEANENEQAYICTYISSLPSLNSEVKEDVVEEYVDKLIDKFEVNTAIK
                     LESKHEFKKNILRHLHSSYNRIRFKFPIRNPMLDETKYKHESLYKIIKSIIENEEEFP
                     VFEGIREEEIGFIAAYFGGYLRGSRDNGLRRNKVLLVCPNGLMVSKSLEIQLYKYIPT
                     IEIVGIVSIKQLKEVNVYYDYIITTIDIQNVNNVIVVNPLLTSSDVQLLMNKLISVKE
                     NEKYFNLELIIQAIRKNGVINNEEALKADLLNIIHKIDEGEMYQPMLKELINAERVNI
                     IKSVRDWKEAIKIASKPLLEDNSIEELYIENMIKSVEKYGPYIVLADRFALPHASSKE
                     GVNKLAMSLLIVEDEVDLLGKPVNIFMVLAAVDNTTHIRALASLSEMMYEEENVKLII
                     NGDKSSILELINKQN"
     gene            21168..21455
                     /locus_tag="MJE33_000399"
     CDS             21168..21455
                     /locus_tag="MJE33_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY9646379.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIQKALKQLGIECEVENTDINSARG
                     ADCDCIFTSQELLNELKGTCNVDVYPVKKYMDLAEVTEVVQTFLSDKNDKK"
     gene            21588..22847
                     /locus_tag="MJE33_000400"
     CDS             21588..22847
                     /locus_tag="MJE33_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBY9646380.1"
                     /translation="MLDFISNNILRNPSMLIGLIAAFGLVLQKKKASDVIKGSLLAAF
                     GIIIMETGTGMLVSSIAPINGAFQSISGGAVTKGLSDVTFTASYGGIIGLAMFAGLAI
                     HLMIARFTPIKTIFLTGHMLYWFPFIFVASGVEAGLNGGVLIAFAAVLSALYWSIMPW
                     LLRKYVFDVIGDDSFTLGHPSGCLALISGFVAKLVGNKEKSTEDINIPQSLSFFREIS
                     ISGGMAVFLMFIVVGIVIPGAFKDSNEFIGTYAIKQGLSFGAGLIIMLHGVRMLINQI
                     MPAFQGISEKVVPNALPAFDCPILFNYKPNAVLMGFLVAMLTSTAMILICNYFNVFNL
                     LLIPLVITSFFECGAAAVIGESQGGIRGAIIGTAIAAIFMVVLVGISAVIYSGTIQNW
                     ILIFGGNDLSLFGIISRFIGSLFGGIL"
     gene            22850..23590
                     /locus_tag="MJE33_000401"
     CDS             22850..23590
                     /locus_tag="MJE33_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBY9646381.1"
                     /translation="MKFKYIDEIRRLIDIIENEEKVNMEKAVQIFVEAIESKNAIFSF
                     GASHAGILTEELFYRAGGCVLINPVFAKSLMLDTAPITHTSKMERLEGYGTALADKTP
                     IKKGDVVIVHSVSGRNPVSIEFAMKAKENKAIVICITNLSYSKSVSSRHSSGKKLYEV
                     CDLVIDNHGQKGDACVEIDGLNQKVSPTSTVIATTVMNSIIAQATQELVNKGLKNPPI
                     FYSANIDGGDELNKKIFDEYKSVIHYEY"
     gene            23913..25223
                     /locus_tag="MJE33_000402"
     CDS             23913..25223
                     /locus_tag="MJE33_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBY9646382.1"
                     /translation="MKKENKMEGIKVGMLKKLCSLAMVAIMTTTLITGCSSGNGKDSK
                     KDGEKEKLVLWMPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISA
                     GEGPDVGYMYAEMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAA
                     NPGVLYYNKDILEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFG
                     NLNWNWYPYLWQAGGDIFNDDLKSVKFNDKSGLEAANFLKELQAYVPEDSLSKDSNEM
                     IESVFGPGKAAFTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLMSS
                     AKNKELAYKLIQHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKNVYRPL
                     VVGPHGVEIYEYLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            25321..26238
                     /locus_tag="MJE33_000403"
     CDS             25321..26238
                     /locus_tag="MJE33_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBY9646383.1"
                     /translation="MENLVREFEENSKRKKTKLNIKPYMYILPLGIILVAFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTVVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSKTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGSIWSLQVFDLIYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVRRDNSDF"
     gene            26254..27063
                     /locus_tag="MJE33_000404"
     CDS             26254..27063
                     /locus_tag="MJE33_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBY9646384.1"
                     /translation="MKTARRLLSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFFNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDAMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            27121..28869
                     /locus_tag="MJE33_000405"
     CDS             27121..28869
                     /locus_tag="MJE33_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HBY9646385.1"
                     /translation="MEISYVLKDCTFKHNKYSVVEAETWKDKDLDIVVAKGEKFAFQI
                     MLKATEEFNCTIDKSSAISWKGLGNRVRLALNMPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDVLVEQYLPQTFWIEGQVPEDFEENNLDIGIDIFKSFGYEDEEKVCTIDVP
                     VKVRNVVLKPLDESKFFLDLWQHPSCLARMYKVELWSDIHFEIVDNYLKELASLGEKV
                     ATVIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKGLDGKIKCNFESMDRYISIADKY
                     KMAKEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDEKDKVFKFINNTNDLKEYIGL
                     VLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVGTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIESLKKKINDKGGILTWFVCCFPEKPNSFLSSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     MENLRFGIQDFELFTMLEKEKGREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNW
                     GYDKVKKYVLELLDRV"
     gene            29131..30153
                     /locus_tag="MJE33_000406"
     CDS             29131..30153
                     /locus_tag="MJE33_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HBY9646386.1"
                     /translation="MFGGINIKRIGIIIMFFLSISFIGFYLVIRDNSDTVKMQRGINI
                     GNALESPKDFPWDVKMSNKFFDDIKDAGFDTVRIPVRFSDYTSDSDNFKIDEEFFKKI
                     DKYVDYALDKDLIVVLDLHHFEEIMKEPRVHKEKFLKIWQQIANRYQKYDKKLVFELL
                     NEPKENLYSQLLNEYIEEAIKIIRKTNPKRTIIVGPYNFYQIDYLNELNIPKDSNIVV
                     SFHYYEPNDFAFQGNIYHKGFEHLSNITWEGTNEQMDYLKKRFDTVENWANKNKVKIF
                     LGEFGITKEAPEASRRAWIKAVREEAEKRNFSWAYWELASGFGIYNQIEGTWDRDILS
                     ALIEKR"
     gene            30158..32464
                     /locus_tag="MJE33_000407"
     CDS             30158..32464
                     /locus_tag="MJE33_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBY9646387.1"
                     /translation="MKKNSKKILITLTIITNVIYILWRIFYTVPKEEGKFALICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPIISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDKKKVHIYICDDGNREEMCILAEKMGVNYITRTEREGAKAGNLNNAMQHTNS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSEGRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPEVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLVFWLPMYILS
                     SLSLKIFSQNIRNTRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKSKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSMTYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYIAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEVEFRE
                     KSGNEMYIANMKAKIVNVVEVNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKTRTLSRRSPRINMNFQMDIKNTGKLRIVNFNYQYVLLNF
                     ENKSIYPKEIALEINENIVLECDLCEGKIDERGILYKVNNIDSIMQNFFLRDEMMDWI
                     LQNKKILDSKPSEKKEKSIDEFEPMEYI"
     gene            32436..33551
                     /locus_tag="MJE33_000408"
     CDS             32436..33551
                     /locus_tag="MJE33_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646388.1"
                     /translation="MSLNPWNIFRRDEMKTLKVFFCSTKYLILVFIFFINTTFISSAT
                     SDKILDLSFKKIETDLSSKITYEDTGVKIETDSSKSDKERYLYIYQNIKENWSMYNNF
                     YIEIQNKNKSSQRINLSIQSKNMLEFRLKEGSEVFLEGKNIIYSDKIKEGCIEVPGEF
                     EGKIYVNFNSLINEESNVVLDSNMLSNIVSWGITFIPSDEEHNIVIIKKISLLSEEKL
                     RFLNNIKIIGDEEVQIPVLGQSISQYEVLGLKSDSKIKYSLMGKQDNVSISQKGKLTL
                     SNKAKPGQIILQVNVDDKFKIGKKITLTESWSVNKKDKDGVPYTLVYPEQSPTVQDMK
                     KINFMNNIITFVRILFVSLVIICFGIYLYWKKCSKTK"
     gene            33569..34987
                     /locus_tag="MJE33_000409"
     CDS             33569..34987
                     /locus_tag="MJE33_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HBY9646389.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYVFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMIVSIIMNYIFGLLVDRYRESKIKVKVFLVLMCAYNIGVLFIFKYLAFAL
                     RNISELISTELTIPSIVLPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTLQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRSPNLKEAYNYFKAMFGG
                     NGAIWSDYTYMFLKEYWVFFIFAFIFSVPIAKKINKFVVEQAKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            35002..36210
                     /locus_tag="MJE33_000410"
     CDS             35002..36210
                     /locus_tag="MJE33_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646390.1"
                     /translation="MNRFFKVRLITAIMFLIILFAFSIFNVCYSGKDTIQEIKNSIAE
                     KSSLKDTISSIDNTVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSVVKDTSGKMHYTY
                     FAKAPNPVGVIADRVRKFSDEMEKQNTKLVYLMTPDKYIKGVTKFPKGIPYSYSNETA
                     DNFLNQLKKYNVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFGELVEQLNKK
                     YNMNLDENHYYRDKDNYNSIVYPKSFLGSMGRKTGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKSQKFELKGRFEESLILSYPFNADLDLMDGQSDKYFTYLLGNRPLVKIKNIENPD
                     GLKVLFVKDSLIVPTAAFFSSVCSSIDMIDPRYYDGDILEYAKSHDYDFVFISVYPQN
                     LTKEFFPFCE"
     gene            complement(36284..37672)
                     /locus_tag="MJE33_000411"
     CDS             complement(36284..37672)
                     /locus_tag="MJE33_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HBY9646391.1"
                     /translation="MESSFLSQFLMITDFKTIFFIILLLGTFFIVRQFEKKKIKFSLR
                     TIYATVLGLILGIIIQAFAGFPNDTTQVIWLQEVSKWYSLFGSGFMDLLKMLVVPLVF
                     LSIIKVIINMKDNNLGTLTFKSLGMLLATTAIAAIVGIIVANIMKLGVGANMPTLVEN
                     QELKEINSLVDTLRGLLPSNPVMAMANGNIVATIIFATFIATSIKRLSKKHFETIKPF
                     IGFIEASYKIIVSVSMTVIKFMPYAVVALLANTITSQGISSIVSVLQFILALYISVAI
                     LFIIHLIFITLNGLNPITYIKNSTEALLLAFTSRSSLGTLPVTIESLVQNHGVDEGVS
                     SFTASLGANMGMNGCAGIYPALMAVTLANMAGVNMDMSFYVMLLIVITISSFGIAGLP
                     GTATMAVSVVISGVGLGSYFPLVGGILAIDPILDMGRTMINVNGAMVSSITVGKSLGK
                     IDKEVFSKKNIS"
     gene            38013..39347
                     /locus_tag="MJE33_000412"
     CDS             38013..39347
                     /locus_tag="MJE33_000412"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HBY9646392.1"
                     /translation="MRVIIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFNDADELLARTPEKLREAGIDLNIFREVVEVDSESKKIKVKNIKTDEIYED
                     YYDKLMIATGARSIMPPIKNIKLKNVSTLKSLYDGEYLKKLLSNEDNKRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEMTDVLEEEINRHNVNLHLDEMVVELC
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSNTNIKMLKNGAIVVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREISYQGSLSSSCIKIMD
                     MEAASTGITERQAKDLGINVKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVILGGQV
                     AGFKDAVQRANVLAAAIFGKMTTNQLGMLDLCYAPPFARTWDVLNVAGNVSK"
     gene            complement(39489..40406)
                     /locus_tag="MJE33_000413"
     CDS             complement(39489..40406)
                     /locus_tag="MJE33_000413"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBY9646393.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILIDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPVGIVDCIRSMR
                     NLVEYVPYTLKIIENPQGNFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLDSKQYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKDSDLFICEAMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDIP
                     SMYLENATNVFENTILGEDRLSLRLNFDE"
     gene            40614..41498
                     /locus_tag="MJE33_000414"
     CDS             40614..41498
                     /locus_tag="MJE33_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HBY9646394.1"
                     /translation="MKVLKKITILSLITLTLVGCSNGEDKKVVEGSKKIQNSKKDEQP
                     IIVHKDANILKDRYEPPKGFKRVDVEKGSFESFLRNTKLKNYGEKVKYYDGRTKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWAQGY
                     RISVKDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGEKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
     gene            41711..43552
                     /locus_tag="MJE33_000415"
     CDS             41711..43552
                     /locus_tag="MJE33_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HBY9646395.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLERFEEITIFHTNDIHGRY
                     AEGDDHIQIGNLATLKKETPNSILVDAGDCLHGLPIVNMDRGKSAIELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGENTLKFLSSNIFENGKKVFDSNDIKEIDGIKIGFF
                     GLSTQETKSKTGFKNVEGLEFKNPIESATEQVNELKGKGANVIVAICHIGTNSLSKPN
                     SIDIANEVQGIDLIIDGHSHTKFENGKQVGDTLIVSAGQYLENIGEIKLTLDTNDVEN
                     IKIENKSVRLIGKEEALKYKLDSAVSKKVDEIKEQQEKILDKVIGVTTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITRRDIVDVFPFNNTIVTK
                     ELTGAQIKDVLEHGVKLYPEKSSAFLQVGGISYYFNPKQKEGEKITNIQKEGKPLDLN
                     KKYVVATNDYIASGGDEFPCFSKEPVLKEFGNLESAVIEYIEYKTEISKNVDGRIGIK
                     SKDELISKNHESDLILSENEKNINDNEENLSNNIKSSDSDKNKSLDKTSARESSNLGI
                     SKREAKEKSPKTGDLGFSNSIIIFIVSSTLICLLNFNQKELKDKKSK"
     gene            43857..44564
                     /locus_tag="MJE33_000416"
     CDS             43857..44564
                     /locus_tag="MJE33_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY9646396.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFGIEEAKNIFAKE
                     EICLIISDIGLPDGSGFDFCEEIRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFYYIENKLIIRTDDKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDSSGNFVDDNTIAVNIRRLRQKVEKNPSEPKYIK
                     TVRGVGYMWGERSIKKC"
     gene            44558..45868
                     /locus_tag="MJE33_000417"
     CDS             44558..45868
                     /locus_tag="MJE33_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY9646397.1"
                     /translation="MLKNMNLKDGLSVKDDYIMRRVIVYSVLSVLIVTLVFILFEYSR
                     LHKLYEHQIKNTQNIAGILIEKYPNDEIDIVNSIYNSDYSKVSTGEKAFKKFGYGLEN
                     KMSNDKNFSLYLKSFFKESFFIFLMMFIVVIGIIYYFIRYINKRLSKIYFIVDKMSQE
                     NYLKDTTESEETGYLVEYNEYFKEGIFSKINNCLYELNRSLKIKFIKLEKEKESVKSL
                     VTDISHQLKTPLASLKLYNTLLIEEELEEDEANEFLLTNKASINKLENLINSLVNISR
                     LEISMINIKKEDNDIKNTILNAVKSVTPKAKSKSITLNLNEFNSRIIPHDKKWTEESI
                     FNVLDNAVKYTQVNGEINISVEETANFFKIIIEDNGIGIEKNEFNNIFKRFYRGSADE
                     IESVEGSGVGLYLSRKILEEQGGNIIVSSKVGIGSKFSLFLTTM"
     gene            45985..46668
                     /locus_tag="MJE33_000418"
     CDS             45985..46668
                     /locus_tag="MJE33_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9646398.1"
                     /translation="MKILYTENLSKHYGKGESLVRALDNVDLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDESFIKELLKSLGLENKHDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGR
                     VSKVGDKND"
     gene            46661..49048
                     /locus_tag="MJE33_000419"
     CDS             46661..49048
                     /locus_tag="MJE33_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBY9646399.1"
                     /translation="MINTTKIAKSNLKQNKSKSILIIITIILSTLMLSSIGIYIVDAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGEMNGVGVEKLEDDSNISLA
                     YMNEDALKLNSFEFIKGKLPSKENEIVLDSGALKALGYKNKDLGEKIKISYDDYKSDK
                     KIEKEFIISGILKTSEISEAGKYYYAIISESYMRNTRDMSQEDFNIYVKLNDKSNLSI
                     EQAKSKLNKIANDVGLDTINVAVNENYINALKPDMETIMGGVFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQVKNIVFKEGFILAGIAIPIGIILGYVLANIIIK
                     SFMDIDVKSSRLPVILSVVVISFISVVLSLLKPMKVASKVSIVDAVRYTGNKISNKSK
                     RKGYKNINLKRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKDNLNILQMDNPLSKDFFNQIKNMEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLNKNIIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTEEG
                     KYQEIKDSIKKIAKNNGFLETDFIDSRIESNKATISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAMYYTSGMMIGSILFGGILGYIAV
                     IFLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLIDRVRYSE"
     gene            49328..50470
                     /locus_tag="MJE33_000420"
     CDS             49328..50470
                     /locus_tag="MJE33_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBY9646400.1"
                     /translation="MNKKINYAPGPTETRENVRLARAEKTTNPDIDIDFVEFYKKTCE
                     KFGNIIGTKNDVYILSGEGILGLEAACASLTEKGDRVLVIDNGIYGEGFKDFVTMYGG
                     EYVLFSSEYTKSIDIDELKAFLDEDSNFKYATVVHCDTPTGVLNDVSKICPLLKEYGI
                     LTVVDSVAGMVGERLSVDESKIDIILGGSQKAISAPAGLTIVGISQDAKNCIKNRKTD
                     VIGFYCNLNIWEGYYEKKYFPYTMPISDIMGLDKALDNILEEGIENVLNRHEKIAYSV
                     RKSIEEYGLELFLEEGYSNTVTAIKIPESIGALELTDYMLKNYNTLVATSLNQYMDTI
                     LRIGHMGENANLNKIEHVLNVLDKSLSALGFKGNGNLLNLFNKYYF"
     gene            50489..51013
                     /locus_tag="MJE33_000421"
     CDS             50489..51013
                     /locus_tag="MJE33_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBY9646401.1"
                     /translation="MEVTAKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIVFGSKKGALSGAIGMSLFDLISGWTLWAPFTFIDRGVMGYLLGKIA
                     WANGNNGNNFLINVIGICVSSIWMLFGYYVTEVILYGNFIVPLTSIPGNLMQVLIGLI
                     IALPISKVLKKCIK"
     gene            51113..51931
                     /locus_tag="MJE33_000422"
     CDS             51113..51931
                     /locus_tag="MJE33_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBY9646402.1"
                     /translation="MLSVTRYSIKNVRLKPWVKFIWYFETKSNILLNNKLLPTDSIDI
                     ILNLSDVMEYKIENQDYIASNIHFNGIRDKHGFIIQHGNIRVIGISFYPFGLYPFLKI
                     PISEFNRQIVDLEAVSKLFAKKLEESLNPTKAIEKVVLCLEEVLLSILEEDLISNKYV
                     KLLNSFIYVNRYSNIKAFCDDTNINIKTLERICLKYTGYTPKILKRIYRFKMASNQLI
                     YNYKDDELFDFIYENEYYDQAHFIKEFKKFSGTSPIKFSGENKTIKENIKYSYL"
     gene            52009..52320
                     /locus_tag="MJE33_000423"
     CDS             52009..52320
                     /locus_tag="MJE33_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBY9646403.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSVSSKMFKDSSTEKFRKALKNQTVKMNIIPQIKSWK
                     L"
     gene            complement(52497..53180)
                     /locus_tag="MJE33_000424"
     CDS             complement(52497..53180)
                     /locus_tag="MJE33_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646404.1"
                     /translation="MQKEVAMNKLIKDCVNFFNDCGFSYSICGGYALELFANMHIRPH
                     SDIDIWISDTDKEKSIQYMLNQGWDIYEPLGNYLLRPIIDLNKQHIEQICVFAIKPDC
                     SFIELKKVEDDNYKMNIQCDEQLQFDFIELIYNPQKDGKFLFSQNQRIIRDMDKARLI
                     TSDNIPYMAPEVVLFCKSIYIDRLGYQKDFDVTVPLLSQEQRDWLVNALITAYPDGHA
                     WIDQLANQY"
     gene            complement(53273..53914)
                     /locus_tag="MJE33_000425"
     CDS             complement(53273..53914)
                     /locus_tag="MJE33_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY9646405.1"
                     /translation="MGRKKEPDNTIQKILDISHKLFLEKGYEQTTIQDIVDNLDGLSR
                     GAIYHHFKSKDEIIKAITSRLYNDGFYGYEVKNDEGKTALEKLQYRLLHAYEQHNKQD
                     IFLMSSSLLRNPKYLATQFDEIINDIVPWIKALIEEGNQDNSMEVKNPEIAAQMFGFL
                     LNTWCIPTVYPGNPQQMIERFTLFGEQAAFMGISFMNDKIMCEYQKFVNKFSK"
     gene            complement(54088..56091)
                     /locus_tag="MJE33_000426"
     CDS             complement(54088..56091)
                     /locus_tag="MJE33_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HBY9646406.1"
                     /translation="MKSQKRFKILDIDVYLQPFESDIKKRMKHYEDMKSKTLKNYKDF
                     SSFANAHLYYGFHQTNDGFFYREWAPNADSLSLIGDFNNWNRKSHPLRKIGSGNWEIF
                     IPGQNSLPHKSEVKVQVTANGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTPFIWTDN
                     DFDLKNITSPLIYECHIGMSTESESIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYHNHGLGVSFDSYEKYFSMNTDIEAITYLQFANELMKEIKPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLAMGVPDFWIKTISNLSDEDWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNHVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLNFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYSKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDM
                     VEFGGNTKKENLRDYDVYFSSTPPSSKMPIASRTAIVLLDNGI"
     gene            complement(56244..56882)
                     /gene="recX"
                     /locus_tag="MJE33_000427"
     CDS             complement(56244..56882)
                     /gene="recX"
                     /locus_tag="MJE33_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBY9646407.1"
                     /translation="MGIITKIEQQKRNDDRVNIYVDDKFFIAIFKELVYTFNLKKGNE
                     INEEELKPILDDEMYIKAKNKALNILSHADQSEKKLKEKLSSEFDENIIERVIDFLKS
                     YNLVNDSVLAQKIVNTNVNLNKCGKNRIKQNLYNKGINRSTINEAVSELDKDTEFENA
                     MYLAKKRYERVKKEDKKKIYQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
     gene            57158..57847
                     /locus_tag="MJE33_000428"
     CDS             57158..57847
                     /locus_tag="MJE33_000428"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBY9646408.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
     gene            57848..58420
                     /gene="yqeK"
                     /locus_tag="MJE33_000429"
     CDS             57848..58420
                     /gene="yqeK"
                     /locus_tag="MJE33_000429"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBY9646409.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSKNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDTLRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            58537..58887
                     /gene="rsfS"
                     /locus_tag="MJE33_000430"
     CDS             58537..58887
                     /gene="rsfS"
                     /locus_tag="MJE33_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBY9646410.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
     gene            59241..61661
                     /locus_tag="MJE33_000431"
     CDS             59241..61661
                     /locus_tag="MJE33_000431"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBY9646411.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDILDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVEAFVQKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMINSEEFNGLDASEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNIVVK"
     gene            62514..63182
                     /locus_tag="MJE33_000432"
     CDS             62514..63182
                     /locus_tag="MJE33_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBY9646412.1"
                     /translation="METVTNNQSGVEIQLSLFEAIKDLLLINPIFLLGIILIGIFLVV
                     IFKKNSDIPKVKTSILSLAMYYYLCLTLSHIVGIPTFSEYIRLSQLGETFFNPNINLI
                     PFSDGFSLSFILNILLFVPLGFLCPLISKTFERLRNTFLIGFGLSLFIETIQLFTLHR
                     VTDIDDLLTNVIGTIIGYFCFKLIAKLRIVKLHSDDDSMEQDYTVCIPIAIIIVALIL
                     GFFS"
     gene            63222..63302
                     /locus_tag="MJE33_000433"
                     /pseudo
     CDS             63222..63302
                     /locus_tag="MJE33_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005541856.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(63665..64782)
                     /locus_tag="MJE33_000434"
                     /pseudo
     CDS             complement(63665..64782)
                     /locus_tag="MJE33_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861579.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            64998..66368
                     /locus_tag="MJE33_000435"
     CDS             64998..66368
                     /locus_tag="MJE33_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp29"
                     /protein_id="HBY9646413.1"
                     /translation="MKLKKLLSLGLVSMFVAGFPISANALDKIDKIQGADKYETAGII
                     ADKQSYTTAILINADSTMADGLSASGLAGATNAPILLTKKNNIPNATLKRVEKAKKVY
                     IIGGESSIDKATETFLKDKGIETRRLQGNDRIKTSYNVAKEINSINKVNKVILTNAFK
                     GEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGIESYAIGGTSSMSDSLVNDTNSTRL
                     GGVDRYDTNKKIVNKFYNGAKEFYIASGTDLVYALVASPMAKNNPIVLVDIGSNKDIL
                     KNAAKVTGIGNLSDKIMLECENTINNISLPSSNKNIICGLKIDFDSNHIIWQDKGSYL
                     ECYDDNLEYYTEKYKDPTVPINIENTSTQNLDVTKLKVYGEANSTLYMGQYAKIKMDF
                     NSFGVLKPGEKCTIYLDIISLDPSRSMTLTFSYVGDKENYKYEQGLASRILFYNPDGE
                     DFFEYK"
     gene            66662..66820
                     /locus_tag="MJE33_000436"
     CDS             66662..66820
                     /locus_tag="MJE33_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646414.1"
                     /translation="MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDL
                     KIRFHKTK"
     repeat_region   67106..67731
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=67106..67134
                     /rpt_unit_seq="gttttatattaactatatggaatgtaaat"
     regulatory      complement(67872..67969)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            68196..68579
                     /locus_tag="MJE33_000437"
                     /pseudo
     CDS             68196..68579
                     /locus_tag="MJE33_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     gene            complement(68757..69752)
                     /locus_tag="MJE33_000438"
     CDS             complement(68757..69752)
                     /locus_tag="MJE33_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBY9646415.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGRGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDEIKDFFEKGIYC"
     gene            complement(69873..71501)
                     /gene="aspD"
                     /locus_tag="MJE33_000439"
     CDS             complement(69873..71501)
                     /gene="aspD"
                     /locus_tag="MJE33_000439"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HBY9646416.1"
                     /translation="MKNHTYKEIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAG
                     RGNPNWTCSTAREAFFTFGHFAITETRSNWDLGHLAGMPQKKGIKERFFKFINENIDM
                     PGAYLARDIINFGINELGFDGDEFVHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQE
                     MKYDISGKYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPN
                     LPRYDFKVVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDET
                     SCNNLIDVIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWR
                     LGTFALHKKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNH
                     TAGLSTPQQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAA
                     YYTQFDIEEWAKLNYGEDLFKFISKTASPVDVLYKLANDYSVVLLSGNGFYGPEWSIR
                     ISLANLYDEAYTKIGKAIREILNGYIVEWQKIKK"
     gene            72160..73377
                     /locus_tag="MJE33_000440"
     CDS             72160..73377
                     /locus_tag="MJE33_000440"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HBY9646417.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKGYGAEVVLNGAVYDDAYAKAVEIQKETRATFLHPFNDKYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKIVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            73472..73852
                     /locus_tag="MJE33_000441"
     CDS             73472..73852
                     /locus_tag="MJE33_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBY9646418.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            74136..74618
                     /locus_tag="MJE33_000442"
     CDS             74136..74618
                     /locus_tag="MJE33_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBY9646419.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNSINIEKVNDSVFSKKLMGDGVAII
                     PNSNVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVSLQGEGFKNEVSQGD
                     TVKKGSPIISFEREKINSQGIDCTTIIIVLNHSEFSEINCMVENEVVAGQDTVIEIMK
                     "
     gene            74639..75454
                     /locus_tag="MJE33_000443"
     CDS             74639..75454
                     /locus_tag="MJE33_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBY9646420.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKVNEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEIIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSIDLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNVYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            75505..77055
                     /locus_tag="MJE33_000444"
     CDS             75505..77055
                     /locus_tag="MJE33_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBY9646421.1"
                     /translation="MKEKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     LPFFKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQLAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGCIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQGIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGADTVSGAYAIVMTEMNLPVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATIIPLIITAVLASVTEPLDF
                     MFVFCAPFLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSIIMNTTVGVEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEEQSKQLNLDAGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEAY"
     gene            77152..78504
                     /locus_tag="MJE33_000445"
     CDS             77152..78504
                     /locus_tag="MJE33_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBY9646422.1"
                     /translation="MIITIVGGGSTFTPGIVKSIALREKELELEEIRLFDIDKERQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKDYWILNYSNPAAIVSEACRKLRP
                     NARIINICDMPIAIIDMIAGSLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWESVYTMLESFPKYLPNTYLTY
                     YLLSKETVEHSNINRTRANEVMESREKDLFEGVRHYLETGEISEKAFYAGSHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEIPAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELR
                     "
     gene            78635..79393
                     /locus_tag="MJE33_000446"
     CDS             78635..79393
                     /locus_tag="MJE33_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY9646423.1"
                     /translation="MNIINKIFWEEDRIGMITNNLEADTHSHCMLQLFLGIEDSIEIT
                     VNEKLVKCNCIIVDKNISHSFSARKKVYYSAIIEPTSIYAEQLTSKMNDFGYWICDND
                     GLEKLRQQGTFLIDNSSKEQYLRFMEMLNNYLNIPITLKHYDDRITELLNLLRTCNCD
                     NHTISSFADKVSLSASRLSHLFKEQIGIPLKSYILFHQMECAFRELLSGKNVTEASMT
                     AGFDTPSHFAGTVKRMMGMPVSLSLKDSEFLKVY"
     gene            79440..80159
                     /locus_tag="MJE33_000447"
     CDS             79440..80159
                     /locus_tag="MJE33_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBY9646424.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKNWLELDDFHKIKSIYEFV
                     QNDILFGYNASDMLSATQVLNDGMGQCNTKATLLMALLRAVNIPCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFFNHGGTFKKY
                     AIATNDLKNTSIDWDGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHMSKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(80283..81683)
                     /locus_tag="MJE33_000448"
     CDS             complement(80283..81683)
                     /locus_tag="MJE33_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBY9646425.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADIGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLIARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLFLSPIAILDVILSFILLRNVGETQKLKLDIPSIVLSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPILIGIMSLILFTLRQLKSKEPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPIAGRHLDKYGPHALSI
                     LGTGCLFLGTLSFAFLGRDTSLIHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINNTLRQISGAIGSAILITIMTSATKKAHMSSNMLSNIHGIDVAFSIAATLAFTG
                     LIVSICFIKRHQIIRS"
     gene            complement(81687..82595)
                     /locus_tag="MJE33_000449"
     CDS             complement(81687..82595)
                     /locus_tag="MJE33_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY9646426.1"
                     /translation="MKGDNKVTEKQRLIITIAQKIFDQKGFQNTSISDVVKECKMSKA
                     TFYKHFETKESFICEIINYYDEKFLEIIHSINENNDIPSSEKLNRKIIAVWKNIFSRT
                     TINTYIRENFSEEQRKATSKLQTKSRANLLNEYKLSLFDNYGDKIEKIIFDLVFLLDA
                     LIHQFIYIIHVQKREINVYFIAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEVYF
                     SLHNKSLFFKTIQDIEELIKTDTSLLENPKLLEAIKKLYVEGKNQQYDSLIMDAMIAY
                     LEKEDTLRPKVLLLNSIKNQLKKETL"
     gene            82856..83503
                     /locus_tag="MJE33_000450"
     CDS             82856..83503
                     /locus_tag="MJE33_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBY9646427.1"
                     /translation="MKKKICVAIIFALFIVFVEMFIDSSSEGSKIMTAELSKYRKGVN
                     VLSINNIIVANKKYSLPKDYSPQESSEARDAFYKMNKDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYVKEHGEKEANRFSAKPGESEHQTGLAFDIGGDDQSCWANKKFNNTKEAKWL
                     YENAYKYGFILRYPEGKENITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            83828..86467
                     /locus_tag="MJE33_000451"
     CDS             83828..86467
                     /locus_tag="MJE33_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBY9646428.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQSLKVVE
                     GMLTGESEPVLKHIEKISEDVSLGDQKNMVFSGAVVVYGRGSFVVTGTGMNTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILALLIFIIQVARNYFMAGNGEMTQIILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEEDFIPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     EGDLALTGKELDALSEEQLYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSIVFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLKAVAIRGCIIGVVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVINLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQLGIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(86525..87628)
                     /locus_tag="MJE33_000452"
     CDS             complement(86525..87628)
                     /locus_tag="MJE33_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HBY9646429.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCGEGINTVSYSNK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFIALDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIPLSSIENIVKEAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVSPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPEVIKAFQIIDNIE"
     gene            87989..88810
                     /locus_tag="MJE33_000453"
     CDS             87989..88810
                     /locus_tag="MJE33_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBY9646430.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKSGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTRTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIVKDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEELIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     misc_binding    88889..89100
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            89187..90503
                     /gene="argH"
                     /locus_tag="MJE33_000454"
     CDS             89187..90503
                     /gene="argH"
                     /locus_tag="MJE33_000454"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HBY9646431.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCVLYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLVLDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVGLISEFKATIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELQKFSDAFKEDIYDFIDYESILNKGIKKNLK"
BASE COUNT        34486 a        10513 c        15079 g        30464 t
ORIGIN      
        1 atattaagta tataaaacaa aatgaaaaaa tattaaattt aaactagaag gaggtttcta
       61 agatggacat taaatacaaa taaccagaac aaaagatata aatatatata taaatggaaa
      121 aacttctatc atattgtata atcatatatg taaagaaaat ttataaatta atataaagga
      181 gattagaatg aattcatata atattttggt ggtagaagat gaaaaggaga tagcagatgc
      241 tatagaaata tatctattaa atcaaggata taatgttttt aagggataca atggattaga
      301 aggtcttaag gttatagaaa accaagaaat acatttagct atagttgata taatgatgcc
      361 taaaatggat ggtataacac ttacaatgaa gcttagagaa aactataatt ttccagtaat
      421 aatgctatct gcaaaatcag aggaagttga caaaataatg gggcttaata taggagcaga
      481 cgattatgta acaaaacctt ttaagccatt agaactattg gctagagtta attcacaact
      541 tagaaggtac actaagtatc taaacatggt tggaaataaa gagcaaaagg tagaaagtga
      601 cgatagtgtg tttgcaatag gcggattaga attaaatgaa aatacaaaag aagttagtat
      661 tgatggaaag tatataaaaa caactcctat agaatttaaa atactaagtt tacttatgag
      721 aaatgcagga agagtttttt ctgctgatga aatatatgaa agagtttgga atgagaatgc
      781 tgtaaataca gatacagtta tggtacatgt gaggaatata agggaaaaga tagaaattga
      841 cccaaaaaat cctaaatatt taaaggtggt gtggggtgtt ggatacaaaa ttgaaaaaat
      901 ttaaagataa atatatagtt actattatag tttttatact tatgttatca gcatcattag
      961 gtatgattag tcaatattct actatacaag caagtgctaa aggtggagca gagaatccat
     1021 ttgaacagga aagctttgta gatagtatat ataaaggaag ttatgtttta tatcataata
     1081 tgaaggaaga acaagaaggt aagatgatac aaccatcaaa actatttatc aatgaagaaa
     1141 ctatgaacta tataaaaaat gtaagtaaag aaaaagatac ttatgatggt tcagaaactt
     1201 actatacaga tgatagtatt aatgcagatt ttaatgaaaa ctttgaaaat tgggagcatt
     1261 tcattaaagc gtcaggaaag aatttaaagt actatttagt tgataaagaa agcaattttg
     1321 aattttttaa tgataataaa gaattaggag tacttgatac aacaaaaaat gatgcttcaa
     1381 aagaaaattc tgatgaaaat cagtcaaaaa atacagaaat gaatgatgag gaaagacaaa
     1441 agcagattac agatataaaa gaaaactata gattctacgt tgtaatgaat tttgataaag
     1501 atggaaaagt aaatattgtg gattcttatg gagtaaatca aaaaattatt aatcaaatgt
     1561 tgttatctaa agcaagagaa gatttaatgg atacaagttt aggaggaaat tcaatttcat
     1621 ataagttatc tccaataaaa aatataacat tagtatatgc tgtacctaaa gtaataagta
     1681 aaggtgatag tgtagagtat ggttatggaa atacttattt tgatgatggt ataatttcat
     1741 atataagcaa tgtagaaaat aattcttatt ataagatttc tggaatgatt ttaaaaatta
     1801 tattagctat agttgttatt gtagctttag cattcccata tagaaaatct aaagagttgc
     1861 taggatttga tacattatca agaataccgt tagagatact ctttattgct cttgatatta
     1921 tacaggggct tgtagattta tatcctactg ttataataag taataccttg agtgggaatt
     1981 atgtagagta ccttataaac aatgacataa gtgctaaaat ggctagtata ttggtaagtt
     2041 taatgaattt aacttattgg tttgtaatat tctcaataat atttgtattt ataatacttt
     2101 taaaacatat tttaaatgtt ggaataagag agtattttac aaaacatact gtgacaggaa
     2161 tgttttttgt atggctaaaa aaagcaggaa aaacagttat taatcaaatt agaacaataa
     2221 atctaaaaga aaagccaaat aaaactatag ttacaatttt gggaataaat ttattgatta
     2281 tagttataat gtgtagtatg tggttctttg gaataatact cgcattaata tattcagtag
     2341 tattatttaa aatactgtca gattactcta aaaagacaat atatgaatat aatcaacttt
     2401 tagatgttac aaagaaaatt tcagatggaa acttagatgt aaatatggaa aaagatttag
     2461 gattttttaa tcctataaaa gacgaattag gaaatattca aactggattt aaaaaagctg
     2521 ttgacgaaga ggttaagagt caaaagatga aaacagagct tatatcaaat gtatcacatg
     2581 acttaaaaac acctctaaca tctataatca cttatataga tttattaaaa gatgaaagta
     2641 taacagatga aaatcgtaag ttgtatattg atacattaga cagaaagtca caaagattgc
     2701 aacatctaat agaagatttg tttgaagtaa gcaaggcaaa tagtggagat gtttatttga
     2761 atatagtaaa tgtggatatt gtatctctaa tgaagcagac acttttagaa ttagatgaca
     2821 aacttgcaga ttcatctcta atagtaaaga ataacttttc taatgaaaaa ataatattac
     2881 cattagatag tcagcgtaca tttagagtat ttgaaaatct tataataaat ataagcaaat
     2941 atgcaatgcc aaattcaaga gtgtatattg atattttaga aacagataat aaagtaaata
     3001 ttatgcttaa aaatatgtct gcatctgaga ttgattttag tgtagatgat ataatggaga
     3061 gatttgtaag aggagataaa tcacgtaaca cagaaggttc tggtcttggt ttagcgatag
     3121 caaaaagttt tatagaatta cagggtggta agatgaatat aagtgtggat ggtgatttat
     3181 ttaaagttac aattactttt aacaaaaatt agattagact tctttaataa ttataaagtc
     3241 tataggataa aagagtctgg aaatatttat gtgtattctt taaataaaaa tttataggta
     3301 ttgttttaaa ataatttgta agcattattt caaaacagct tataaatgtt attttaaaac
     3361 agaatgttga tttatttaaa aaaggtgaat cctaatatac aaggttcacc tttttatttt
     3421 acaaaatgtt atttagtgtt acagagctta taaatagcat aatatctggc tttactatta
     3481 acatggtact agcaatttat tttgaaatct tgtataagct attttatatt atttaattac
     3541 tattgtttgt actttacaat gtgataaaga atttacctct acagttttag tatttacttt
     3601 aagcttgcta gtattatcat agatttcgtc gagcattgca ctataagcat cattgacatt
     3661 tttattgatg ttaagacttc ccttagcatc ctcatttttc ataccattga agattctaac
     3721 tacaattcca tctccttttt cttctttctt tattgcactt actgtgaaat ttgaattatt
     3781 tacattcatc aaactaaatt cattttcata tatttgtttt tcgtcattga atgcaaatat
     3841 aagtctacca tttaaaaaat cagcatattc atatatttgt ataggtgtta agaatctctt
     3901 agctgtatta gctacatttg cttcatcaaa tttattgtta tatatgtaaa gaccaaagtc
     3961 aaagtttaac tctttaagta attgtgcatc tggtgttgct attatttttt ctccagaagc
     4021 tcttcctggt ctataaagta aattttctct tcccatatat ccaaatgacc tgaatagtgt
     4081 taatgctatt gtatctaaat tatcaccaac tatttgatat tctcttacac aaccagttat
     4141 taatgctatt cctctttgtc catcctctaa agtaacaaaa ctttgcattg gttcgatagt
     4201 tataggtttt tcttgccatg agtaattgtc tcttttccac aatgcaaggt ctttttctaa
     4261 tactgttggt ctttgtatta ttccaaattg ttgatctgct atagaaaact tagatgctat
     4321 ttctgtatta aataaaactc ttactctatg agatagtgct tggttatttt ttatattaac
     4381 attaaactta attatttgtt ctttaggttc taaggtgatt ttcattgtta ctggcatttt
     4441 cacagaaact ttacctatag ccctttcttc cagtgaagct ggtatggtca atgaatattt
     4501 aagtatcatt tcttcttgaa tgtttgattt tagcacttct actgaagata cagaatctaa
     4561 agatgaaaca taaatatctt ttcttggagg agaatagttg tatgaatctc catcatctcc
     4621 attgtcttca attacagctt ggtttttata agtcttatta gattctttat ctaatatttc
     4681 taatgaacca tttttattgg ctgttatttt gtagtatttg ttttctattt ccttgcaatt
     4741 acttgtttct atttcacatt tatttttagt atccaattca aaaactagtt gagtatatcc
     4801 cattggtggt atatctttta ctgaaagtgc aatttttgct ctatatacag ttttgggtat
     4861 atatattttt ttacttgggt ttaatcttat tgtttgagtt aatatataat ttgtaatatc
     4921 ttctttttct tttattatat aattaagaat ttttccattt ttattcttta tcacaaaatc
     4981 ttgacctgga atataagtat caaattctac aacttcattt ctgacttctg ggaatccatt
     5041 gaatactgtt aatgttattt cttcattatt atttttgatt tgagtactta taagtctcat
     5101 atgcaaatct aataaattta tactcaaatc tctggcttgt ttgtatcttg tgtatacatc
     5161 ctcatttgtt gtgtcactat tacaagaacc tatgctgtca tgagctgcat tttcaaacat
     5221 aagtttccat atatctttta taactaggtg agggtattca tttccaagtg aataacttaa
     5281 tgttaataac ggttcaagta catttgtaat ataattttct aatttgttat tcattatttt
     5341 aaggtctgac cttgaggaga atatggattt gtgtattctc atatgtttag catttaaaaa
     5401 ttcaccttta agctctgtaa attcatttct ttcatttctt actgaatcta tatagttttc
     5461 tatatcactt atttcgtatt cattttcatt atctatttca ttagcttttt ttaatatatc
     5521 aggaaggttt tttcttattg gtgcttggtc aaatccattt ggaaaatata cgttattagt
     5581 agaagcttta accttaagtt ttacgattat atcttttaaa tattctttta aatctgaatc
     5641 atcttctggt atattacctc catagtaata accaaatggt atttgaactg ctaatacttt
     5701 tgaaccatca gaacctttcc atataaactc tgttgtatct accatatcat cagaaactcc
     5761 acgccaaaat aatgaactat ctattccaaa acctctgtat atttgtggca tttgagctga
     5821 ttgcccaaaa gaatctggta catatccaac cctcataggt tcaccaaatt cattgcatct
     5881 atctattcca tagtaaaggt ttctaattat agattctcct gatataacca attggtctgt
     5941 ttgagtatac caaggtccaa taactaatct tcttctttga actaaatctc ttattctata
     6001 tttatcctga ggtctccatt ttaaataatc atctaagaga gaagcttgtg catccatagt
     6061 aaagtattta aagtcttctt tttcttccaa tatgtctaat atatcttgaa aatctttcat
     6121 taaatatatt tttgaccttg atgttgtaaa ataccattct ctatcccaat gactgtgtgg
     6181 aactacatgt acttttgtct ttttcataaa aatcctccca aaatttaatt gagaattgag
     6241 agtacactca attctcaatt atctaaaatt aagtttttaa tttacattat atctaactct
     6301 atttcatcaa attctgtatt ttccgtagtt tcatcttcat tgaagtctac taaagttgtt
     6361 gataatattg ctattactaa agctccaaat attgcaccta ctaagtaagc ccatccagaa
     6421 cctattgata taaatccata taatccacca actggaggca ttatagcatg tacacctaac
     6481 aaagctgata atcctacacc caaagcgcat cctatcatat taacaggaac taattttaat
     6541 ggatttgtta aagtgaaagg aattgcacct tctgttattc caattatacc cataactata
     6601 ttgccttttc ctgtttcagc taatgcattg gaaaattttg attttcttat gaatgtagct
     6661 aaaccatatc ctagtggagg tataactatt gcgcagttta ccaatatcgc tggtagatag
     6721 attccctcta acaataatgc atttcctgcc atccaagctg ctttatttac tggtccacct
     6781 aaatcgaatc ctatcattgc gcctaaaatt aaagccaata ccaccgaatt tgttccattt
     6841 tgacacattt cagctaccca agcatttaat gaagtattta tgtttcctaa aggtcctgct
     6901 aatacaacac tcattaaaaa accagttaat ccaactgtta taagtggcat tattataagt
     6961 ggcatcattc ctgatgctga cccagatatt ttaatttttt tctttaaaaa cttacaaaca
     7021 tacccagcaa agaaacctgc tattactgct cctaagaatc ctgcattagt attacttgct
     7081 atgagcccac caataaaacc tgcacccata gctggtttct cacctattga aaatgctata
     7141 aatcctgcta ataccgtatt taatagacct atgccagtcc aaccaacttg ttccattaag
     7201 taaagaatat gataaaaacc agatacattt ttataagcat ctaaacttgt tatacccact
     7261 gctaaagctg taagctttgg gatagctaca actaaagatg agcctattac aagtggcaac
     7321 atatatgaga tacctgtcat taagtgtccc ttaagttcac ttaaaagaga tttatttttt
     7381 ttactcattt ttaatcctcc tatataagtt tgttatattt tttgataata tgttataatt
     7441 acattagaca attttttaat ttttgatttt tctcattcta tattagagtg agattttttt
     7501 attaagaaat agcttctata cactttgtta agacagcttc tggttttttt aacgctatac
     7561 ctattttgac ttttatcact tttttaccat caaatctttc cattccagat atagtttggt
     7621 cagatgctat aagtataaag tccgcatctt gtgcttcttc ttttgttata gtatttactc
     7681 gtcccatagc accttgttgt tctactttga tttcatgacc catttttgat gcagcttgtt
     7741 ctaaagcttt tgctgccata ggtgtgtgtg ctaatcctgc tatacatgat gttatagcta
     7801 ctattttcat aattattcct ccttcatttt attagttata tattttacta aatgacttgc
     7861 atcactagca tttttaactt cacttgtaaa atcatcctcc attaaacatg tggctaattt
     7921 agatagagtt ttcaaatgta tatttccttc atttttatgt ggggctaaca aactaaatac
     7981 atatctaacc ttactatcat ctaaagtttt ccactctaac tcgttagttg ttttaatata
     8041 gagtatggaa gcttccttta catttacact ctttgtatga ggtatcgcaa atccatcttt
     8101 tattcctgtt ggaaattcat tttctctttt tagaaggtct tttaaaagag cctctttatt
     8161 agttgtaata cctagtatat aagcactatc tgcaataaaa tttaatactt cttctttgct
     8221 ttttaagtct ttatctataa atatatattt ttcttgtagt accatatatt tttccactcc
     8281 attcataatg tcttaactta tatttatatt gtaatcatta tcctaattaa attaaatata
     8341 actaatttcc acttcgacat ggaaatttat attatgataa taactaattt ccatatagaa
     8401 gtggaaataa attattataa tacaatttta ttagagtaat tgtattatat gagtgtaaat
     8461 agtataattt agaagaaatt ctataagggg ggatatttat gcacaagaga ttgctcacgt
     8521 tgtttaaatt attaaatgaa agtgatgata aaattacttg taaaacttta agtaatcatt
     8581 taaaagtgag tgagagaact ataagaaatg atattacttc tataaatgga actttagaga
     8641 aaaatggtgc tattattaaa ataaaaaaag gagaaggata ttatatagat attcttaatt
     8701 tagcattata ccaacagtat ctagcattaa tatctgatga tattatggat tcaagtgaaa
     8761 taccagattc tcctatagaa agaaatcaat atattttaaa atatattctc tataacaata
     8821 catatataaa actagaagat ttagctaata gcttgtatgt gagtaaattt acaatactaa
     8881 atgacataaa aagaataaag cctattttgt caaagtataa tttaatatta gtttctaagc
     8941 catattatgg agttaaagtt gaaggtaagg aaatagatat tcgaagatgt atttctaata
     9001 atatgataaa taggaatttt gaaaactata taattggaat tacagataga gaaatagaat
     9061 tatttaataa tgtagattta atagagcttc aaagggtagt gttaagtgaa attaataaat
     9121 ttaatatcaa tttcctagat tttaatctaa aaaactttat tatccattta gctattactg
     9181 taagtagaat acttgatggt tactgcttag ataatgtttt agatgttgtt ttaacagatt
     9241 ttcaatcgaa tacaactgtt gaaaatatat ttaattatat tgagagtaaa tatactataa
     9301 ttatttcaaa ggcagacaga gtttatctat ataatcattt tataactaaa agttctcttt
     9361 tggataacgt ttccaatagg gtagatacta aaattataga gtatgttgaa gaaattttag
     9421 aagtaatcaa caatcaatat acttttgacc ttagaaatga tagtgtatta tttgatgatt
     9481 tagtactgca ctttaaatca attctcaatt ctaaatctta taatttaaat aaggtgaatc
     9541 cccttataaa tacaataaag agtaattatc ctttagcatt tgaaataact ttaaatgcta
     9601 tagaaaaagt ttttaaaaat agtatttact ctttaacaga agatgaaatt ggatatgtat
     9661 ccctgcatat aggtgctgga atagagagat tttttcaaag taatattaag tgtaaaaacg
     9721 tagtattagt atgtggttca gggtatggtt caagtaggtt attagaggtt caattaaaca
     9781 aagtttttca tgataaaata aatatacttc aatgtctatc atttaatcaa tttttagcta
     9841 gtgaattgtc agatgtagat ataataatat cgacaattcc tttaaatcat gatagtattc
     9901 ctattgtatt agtggattta aaactattaa aaaaagatat agagaatata tctaagtcaa
     9961 tcactaataa ttcccatatt tatagtaatc ttttggacaa tttctttgat aaaaatttat
    10021 ttattgttaa tcctaaaatc aaagataaag atgagcttat aaagttgatg tgcaacaaat
    10081 taactcaaag tgaaattgta tttccatcat ttgctgaatc tgtattttac agagaaagtt
    10141 tatcttctac caatatagat gattttttag ctatacctca tcctatggaa ttaagctcta
    10201 taagaactaa gatttgtatt tccatattga atgaaccaat atattggagt gaaaattcta
    10261 ctgttaaatt aatcatgatg ttggctataa ataaagatga ttatataaaa ataaactcca
    10321 tttatgatat ccttttaaag ataattcatg acaatgatat tagagatagt atatctaatt
    10381 gtaatgattt tgataatttt ctttccataa tcaagtctat agtatagatt tttatattac
    10441 ataagtttta aaaactttaa taaaatattt tttgttttta gcataaatag tataagtaag
    10501 ttttgtataa tttgttatta ttttgtattg attataatta ttatacaata aagattctgt
    10561 ctcaaaatag agagagaaac ctgtttttca ttgataaaat gataaaaata tatctgattt
    10621 catacaaaaa agagtgtctc atgaactaaa aagttcattt tgagacacca cctttattat
    10681 tttatattat ttatctcttg tatatattac tatagcatcc cttaaaaact tagctaatcc
    10741 aggttgttct ctatcataat atgatgtaaa acgttcatca tatacataca tctgtgctag
    10801 agaggaatga gcttctttag agtatgtgtt ccaagtgtaa cacaaccatt tatgatgtaa
    10861 ttttgcaact tcctgagcca gttcactaga ggcatcacca gttttaaaag cttttttaag
    10921 tttactaatt atttcgatat ttaggttttg ccaattttca taatcttgct tagacatatt
    10981 tttaagtttt ttatttgatg tatctataat atctttacca tatttctctc taatttcagt
    11041 tccataattt ttttcatttt catcaatcat cttttcttta aaaccttcaa atttttctat
    11101 atctgtcata gtgtattttc cctccttcag agctatagtt ttagttacat tttctattag
    11161 taaatcaatt tgctttcttt tggctagtaa tttattatgg tgttctttta acgcacttaa
    11221 actatcgaag tttggtgaat ttataatgtt ttttatattt tctaagttaa ttcctaattc
    11281 tctataaaaa agtatttgtt gaagtttatt aacttcattt tgaccatata tacgataacc
    11341 tgaagaattt attttaagtg gcttaagaag acctatttca tcataatatc taagtgttct
    11401 agtacttata ccagctagtt tacttaattt ttgaactgta tattccataa gtagtacctc
    11461 ctataaatta aatatacact tttacgcaac gtaaatgtca atacattaaa atataaaaat
    11521 ttatatcttt taataaaaat ataaaaaaat atctttactg gatattttac acacaactgt
    11581 caatactcac tagtgaaaat taaaaaataa aatttataga ctatctttac taaaaaatag
    11641 aaggtctata gtctaggagg aactgtatga atttaaaaag aagcaatgaa aaacgagaag
    11701 agtacagaag aatgtattca gataaagaaa gttttttaag tttgattcaa aattttacaa
    11761 gtgtctcaat agcaaaagaa ttaactctta aaaatataga gttagaaaca tcttttatat
    11821 gtgaatataa aggaaaagaa gttgatataa tttataaggt attttctaaa agcggcaaag
    11881 tatcacatta tatagtgtta gaatttcaaa cagaaatgga tactgaaatt gtcccaagat
    11941 tgaaatcgta tagagaacaa atttggaaaa gttttattat gaaaaaaagc cttgaagaaa
    12001 tagaagataa aaattttaaa cttcctaaag ttataccagt tgttttatat agtggacctg
    12061 aaaggttaag tagaaaaaaa ggaattttgg atattgtaga atctatatcg gagttcagta
    12121 ataattctag tatgaaaaat taaaattaca taatgtgttt attggtctaa aatggaaaac
    12181 tgtcgcataa acttgaatat gaaaatattt atagatacag gttctatgtg gaggtgttcc
    12241 atgagaaata acaagtctaa ggtattgcta ggaatacttt gtctagcaac aggaatggct
    12301 gtaattctgt caatgttgct tcctggctgg atttggtcag cacttaccgc attaatactt
    12361 attggatgtg gagcattatt atttttttgt taaaatagtt ttaactataa agtgtggggt
    12421 gagtattgat gaaagtagta acaataaaat taccaaaatg gatttcaagt ataatcctaa
    12481 aatttatgag aaagagtaaa aatgattaaa ttaaaaagcc tacttacgga agtaggcttt
    12541 taaacgtggt tactaagctc tttcaatttt accagaacgt aaacatctag tacaaacttt
    12601 tactctctta ggagctccat ctataatcgc tcttacgctt cttaagttag ctgaccatgt
    12661 tcttttatta tgtttatttg agtgtgatac ttggtttcca gaaaccttac ctttaccaca
    12721 tacgctacat acttttgcca tctacgcaca cctccttaaa atgataacta tttaaagact
    12781 aaaaacatat ttatattatc ataaataaca aaaatttgca atagaaatat atatttgcct
    12841 tacacatatt gaagaatttc ttattatcat ataaaattat atatataatt ttatataaag
    12901 taaatatatt tggaaaataa ttattatttt tctttgaata tatatactat agaagaaatt
    12961 ttatccaaaa acttttctag gtttaaatta taccatattt atagtatgat atgattataa
    13021 ttttatataa taaataagta aaaatagtaa aaaataaatt aagaaatgtt tttaataaaa
    13081 ataagcttaa gggggtcaac catgagtgca aaggtaacga atcaatatgg aaatatagaa
    13141 atagataatc aagtaatagc tcaagtcgta tatagagctg caatggaaag ttatggctta
    13201 gtaggtttag catataagtc taaaggaata gtagagctat taaaaggcga gaacgcaact
    13261 aaaggtgtaa gagttgagga agtagaagaa gatgccatag caatcgaact ttatgttata
    13321 atacaatatg gtacaaatat atctactgtt gccaataata taatagatag agtaaaatat
    13381 gtagttgaaa aaatgactgg cgtaagagtt actagaattg atattaatgt acaaggaata
    13441 agagttaagt aatttcatag gaggaaacaa atgattcaat atatagatgg taaaaggtta
    13501 agggagatgt ttatttcagg tgcaaacaat ctccaaaaca ataaagagtt agttgacaag
    13561 ttaaatgtat tccctgtacc agatggagat acaggaacta atatgtcatt aacaatatct
    13621 tatgccttaa aagaattagc aaaagttgaa aatgataata tatcagatat cggaaaagca
    13681 ttatctaaag gttctttaat gggagctaga ggaaactcag gagttatact ttcacaaata
    13741 atcagaggta tagctaaatc tatagaagga aaaagtaaat tatcaacaga agatttagca
    13801 aaagctttta aaaatggttc agacacagca tataaagctg taataaaacc aattgaagga
    13861 actatactta ctgtagttag agaaagtgga gagtttgcaa taaaaactgc gaaaaaagag
    13921 aaagatgtag taaagttttt aagtatgctt gtgaaggaat caaattcttc attagaaaga
    13981 actccagacc ttctaaagaa tctaaaagaa gctggtgtgg ttgactctgg tggaaagggt
    14041 cttgtactta tatatgaagg aatgttagct tctataaaag gcgataacat agaaataaaa
    14101 aatgttgacc tagatactaa tatttcaaca agtatggatt ttgcaaagag tactactagc
    14161 acagatgata taaaatattg ctattgtaca gaatttattt tagaaagttc taaggtagaa
    14221 gatactaaaa taagagacat aatgatggct tatggtgata gcctagcagt agttggagat
    14281 gatggagtca taaaggtaca tgtacacact aatgacccag gaaatgtact tcaagaagct
    14341 ctaaagtacg gtcaattatt aactataaaa atagaaaata tgaagttaca acatgaaaat
    14401 acattactgg atgtagaaga aaagaaagaa aatgatagtg aacctttaga agaagaaaaa
    14461 gagtttgggt ttatagctac ttctatgggt gaaggtttgg caaatatatt taaagatttc
    14521 ggagttgacc atataataga aggtggtcaa actatgaatc caagtactga agattttatg
    14581 aatgctataa agcatataaa tgctaaaaat atatttattt tcccaaataa cagcaatata
    14641 ataatggctg ctaatcaggc aaaagaattg agtgataaaa atataatagt tatacctaca
    14701 aaaaatacgc ctcaaggatt tgctgcatta gtaactttta atggagaatt aagcgaagat
    14761 gaaaataaag aagctatgat gaatgcttta aactcagtta aatcaggtca agtaactttt
    14821 gctgttaggg atacagtgat gaatgaaatt gatgttaaag aaggtaatat aattggaatt
    14881 gcagaaggaa atttattatc agcaggagat tatgtggatg aagtaacatc aaaccttatt
    14941 gaaaagttgg ttgatgaaga tactgctata ataacattat tctttggtga agatgttaca
    15001 gaatctcaag caaatgaact acgtacttct ttagaagaaa agtttgaaga tgtagatgta
    15061 gagttatatt atggaggaca acctctttat tattacttaa tttcagttga ataatgatta
    15121 taagaacctg tatgaccaag tagcttttat aaaattggtt gtacaggatt tttgtgtagt
    15181 aggtgatata tttgttggat ttatataaag atgttcaata tgttaaggga ataggaccta
    15241 aaaaagcaga taaattaaat aaactgggta tatttacact aaaagaccta ttgtattatt
    15301 ttccaagaca atttgaagat agaaataatc taaagaaaat agcacaatta gaagatggtg
    15361 agaaagttac gataaaagca gtaatatcaa gtataaatac atttagccca aaagaaggta
    15421 tgactttaac aaagattgat gtaaaagatg agacaggctc ggctaaatta gtgtttttta
    15481 ataagagtta tataaaaaat acttttcgtc caggagattc aatattagtt tttgggaagg
    15541 ttaagaaaaa atttaataac ttagaactta catcatgtga attagaatat cttactaatt
    15601 cacctaagaa tacatgtaga tttatgcctg tgtaccagct tacatacggt gttacaaata
    15661 aagaaattat gagtataatt agaacagtac ttgaagataa agaattaatt atacaagaat
    15721 atatgccaca aagaattata gaaaaatata ggctatgtag tatagatttt gcagttagaa
    15781 acatacattc accaagtagt aaggaatcat taaaaatagc tttatataga attgtatttg
    15841 aagaattact tatactacaa ctagggttgt ttgtatttaa aagtggcaga aataaagaag
    15901 atggaataaa gtttgaaaca agtaaagact taaaaaaaat aataagtgct ttaccattta
    15961 aactgactaa agctcaaaac agggcattag atgaaattat acaagatatg aatttagaga
    16021 aaataatgaa tagattggta caaggtgatg tagggagtgg taaaacagta gtagctctct
    16081 tagcacttgc aaattgtgtt ttaaatggtt atcagggcgc tttgatggca cctacagaga
    16141 tattagcagg gcaacattat atttcactta ctgagtctct aaaagatttt ggaataaatg
    16201 taggtctatt aataggtagt cttacaaaaa aacaaaagga tacagtattg gaacaaataa
    16261 agaataatga gattgatata ttaataggta cacatgcact aattgaagat aaggttgaat
    16321 ttaataatat tggtttagtt ataacagatg aacaacatag atttggtgtt atgcaaagga
    16381 gcaaactttc attgaaagga gcaaatcctg atatattagt aatgacagca acaccaatac
    16441 caagaacttt agcacttata ctctatggag atttagatat ttctattata gatgaacttc
    16501 ctccaggaag acagccaata gaaacaattg ctattgaaaa aagtaagcga gatagagcat
    16561 ataataatct tgtaagaaga gaggttgaat caggaagaca agtgtacatt gtatgtccct
    16621 tggtagaaga aagtgaagcc atagaagcca aatcagcagt tgaacttgtt gaggagttaa
    16681 gagcggaata ttttcacgac ttaaggcttg ggttgcttca tggtaaaatg aagtcaagtg
    16741 aaaaagatga agtaatgaga ctttttaaag ataaagaaat agatatttta gtttcaacaa
    16801 cagtaataga agttggtgtc aatgtgccaa atgcaacatt aatgataata gaaaatgcag
    16861 aaagatttgg acttgcacaa ttacatcagc ttagagggcg agttggaaga ggtagtcata
    16921 aatcgtattg tgtactaatt tatgattcta aaacagatgt atgtagacaa agaatggcta
    16981 taatggaaga gaccaatgat ggttttaaga tttcagagaa ggatttagca ataagaggac
    17041 ctggggaatt ttttgggaca aggcagcatg gtcttccaga acttaaggta gcaaacttat
    17101 ttaagcatat aaaaatactg aagttagcac agcaagaagc tcgatatata ttgggtgaag
    17161 ataataatct acaattaaaa gaaaacatgg cactcaaaaa agaaattata gataaattta
    17221 aagatacatt gaaagaaatt tcattaaatt aattttaaat tgtgttaaaa tattactaat
    17281 gtaataatgt gctgaaggga gattgtcata ttgagagtaa tttcaggaaa agcgagagga
    17341 ttaaaactaa atactccaaa aaatgaagat gtaaggccaa ctacagatag agtaaaagag
    17401 tctctattta atatgataaa ttcatatata atggaaagtg aagttctgga tttatttgct
    17461 gggactggtt ctttaggaat agagtgctta tctaggggtg ccaagtcatg tacttttgta
    17521 gatattagta aggaaagcat agatattgtt aagtccaata taaaaaaggc aagagttgaa
    17581 agtgaaagta ttattttaaa ccttgatttt aagactgcaa tagataaact taaattacaa
    17641 aatagtaaat ttgacataat ttttatggac cctccgtatt ataaaaatat gtttatagaa
    17701 gctattgaaa aaattgataa ttctaatcta ttgaacgaag atggaataat agttgtagaa
    17761 catgatacga atgatttatt tccagataag atatgtaaat tggagaaaac tagagataag
    17821 aaatatggaa atactacatt aactttttat aagatggagg cataatatgg aaaataaacc
    17881 tagaaaagct atatttgcag gaagttttga cccaattact aatggacatt tagatattat
    17941 ttgcagggct tcgaagttat ttgacgaatt acaaatagga gtattaaata atcctaataa
    18001 aaagggtcta tttagctttg atgaaagagt aaaacttata gaaaaaagta caagccattt
    18061 aaataatata aaagttgtga cttttgatgg tttattaata aattactgtc aggaaaatgg
    18121 gattggagca ttagttagag gtgtgagaag tggtgctgat gttgattatg aacttcagat
    18181 ggcacatatg aatagagagc ttaatccaga tatagagact ataattttac caagttgtac
    18241 aaaatattca tttataagtt catctttaat aaaagaggtt ttgctctttg atgctgatat
    18301 aaaaaatttg gttccaaaga ttgttttgga agaattaaag aaaaaaacta gtgggggtaa
    18361 ttgatatatg aagatagatt tagaaatgat tgaactattt gagcagttag aggagatatt
    18421 tagaagtgct tctacaatcc ctttctcaca taaatgcaca gtggataaag atgaggtttt
    18481 agcaataatt aatgatataa gaactttaat accagaagaa gtaacacagg cagtatggat
    18541 taataaagaa agaaataaaa taataagtca agctaagcaa gatgctacaa atatagttga
    18601 acaagcgaaa aaagaagctg agagaataca acaagaatat caagataata tagaggaatt
    18661 aaagaaaaat tctgaagatg tagtaaaggc atatgtagaa tccagtgaac ctgttgttca
    18721 agcagaccaa agagcaaaag atatagttga tagagctgag agaatagcca atgagataag
    18781 aattgggtca attgaatatg cagaggatgt gctttctagt gttgagtata atttaagaga
    18841 aatattagaa gaagtaaaaa gagataaagc tgaacttaga cctaaaaaat aaattttaaa
    18901 taaataaaaa tggagctatc acacaaaaaa ttccaattta tgtgtgatag ctctctttta
    18961 atatagcaat tttttgttat ataattgtgc tttttagatt acttatatct tattttgata
    19021 ttataatgta gtttaatctt ttatttgttg aaatcaactt atttatttgt ataaataata
    19081 taggttattt tcaaattttt ttaataattt taccactgtc ttcttttaga ttagagttag
    19141 actgtgcaaa attataaaat aatactaaat catgtgcaat tatttggtat ggcactacat
    19201 gtagaaaata tagataataa aattaaggag gtagtatgaa ggaaagggct aaaaaaatat
    19261 tagattttat tattaaaaat aacaatgtaa catcacaaga actacaagaa aagtttaatg
    19321 tttcaaagag aactatttat tacgatattc tagctataaa tgaacaacta ggaaaaagtg
    19381 gtaatataaa aaatgtaaaa cacaaattta tatttgaagg taatttatgt gatgcaagga
    19441 aaataatttc aactgaagaa gataaatttt tagatagtga ttacagaaaa acttttatat
    19501 taaacaagat attacttggc gaaaaaatat caatagaaaa actaacaaat gaaatgttac
    19561 tttcaaaaaa cacagtagta caaacaataa ctgatgtaaa aaaatacctt caaacaatgg
    19621 ggttaagatt agagtataag ggaaagtaca aaattatagg tgatgaatac gttattagag
    19681 agctgtttct gataattgtt caagaaaata tattggaaat taattctata agtgaggaag
    19741 taagtagttt cgatacaaaa ggacatataa aacttactga ttattcttta ttaaatctaa
    19801 ctaagtttgt ggaatttcta aataaaagga taagagatgg gaaaacatta tactcatata
    19861 aatatctaaa tgaagctaaa aaaattagtt actttagtaa ttgtaaagaa cttttatgtg
    19921 aagaagctaa tgaaaatgaa caagcctata tatgcactta cataagttca cttcccagct
    19981 taaatagtga agttaaggaa gatgttgttg aagaatatgt agataaactt atagataagt
    20041 ttgaagtaaa tacagcaata aaattagaaa gtaagcatga gtttaagaaa aatattttaa
    20101 ggcacctcca ttcttcttac aacagaatac gatttaagtt tcctattaga aatccaatgt
    20161 tagatgagac taagtataaa catgagtcac tttataaaat tataaaatca attatagaaa
    20221 atgaagaaga gtttcctgta tttgagggga taagagaaga agaaatagga tttatagcag
    20281 cgtattttgg aggatattta agagggtcta gggataatgg tctaagaaga aataaagtct
    20341 tactagtttg tcctaatggt cttatggtat caaagagttt ggaaatacaa ctatataaat
    20401 acataccaac catagaaata gtaggtatag tatccattaa gcaactaaaa gaagtaaatg
    20461 tatactacga ttacataatt acaactatag atatacaaaa tgtaaacaac gtaattgttg
    20521 taaatcctct tttaacatca tcagatgttc aattacttat gaacaaacta ataagtgtaa
    20581 aagaaaacga gaagtatttc aatcttgaat taataataca agctataaga aaaaatggag
    20641 ttatcaataa tgaagaagca ctaaaagctg atttattaaa cataatacac aagatagatg
    20701 aaggagagat gtatcaacca atgttaaagg aattaattaa tgcagaaagg gtcaatataa
    20761 taaaaagtgt tagagattgg aaagaagcaa taaaaatagc ctcaaagcca ttgttagaag
    20821 ataactctat agaagagtta tatatagaaa atatgataaa gtcagttgag aaatatggac
    20881 catatattgt tttagctgat agatttgctc taccacatgc aagttctaaa gaaggagtaa
    20941 acaagcttgc tatgtcttta ctaatagtag aagatgaagt tgatttatta ggaaaaccag
    21001 taaatatatt tatggtatta gctgctgtag ataacactac tcacattaga gcattagcat
    21061 cactatcaga gatgatgtac gaggaagaaa atgtaaaact aataattaat ggtgataaaa
    21121 gttcaatact agaacttata aataagcaaa attaggagga atgaaaaatg aagatattag
    21181 cagtatgtgg ttttggtgta ggttcatcaa tggtattaaa aatgacaata caaaaggctc
    21241 taaagcagtt aggtatagaa tgtgaagtag aaaatacaga tataaattca gctagaggtg
    21301 ctgattgtga ctgtatattc acaagtcaag aattgttaaa tgaattaaaa ggaacttgta
    21361 atgtagatgt atatccagtt aaaaagtaca tggatttagc agaagtcaca gaagttgttc
    21421 aaactttttt aagtgataaa aatgataaga aatagtataa gttttaaaaa gtaaatttaa
    21481 gttaatcaga ataaaataaa aacttactaa attcaaaaat agtaagaaag agaggaggga
    21541 tttagtggta gaaaacttaa acagttgacc tatcactaaa aaatataatg cttgatttta
    21601 tttcaaataa tattttacgt aatccatcta tgttaattgg attaatagca gcatttggtc
    21661 ttgtactaca gaagaagaaa gcatctgatg ttataaaggg gtcactactt gcagctttcg
    21721 gaattataat tatggagact ggaacaggta tgttagttag ttcaatagca ccaattaatg
    21781 gagctttcca aagtataagt ggtggtgcag taacaaaagg tttaagtgat gttactttta
    21841 ctgcaagtta tggtggaatt ataggtttag caatgtttgc gggtttagct attcacctaa
    21901 tgatagcaag atttactcct attaagacaa tctttttaac aggtcacatg ctttattggt
    21961 tcccattcat atttgtagct tcaggagttg aagctggatt aaatggtgga gtattaatag
    22021 cttttgcagc agtattatca gcactctatt ggtcaataat gccttggtta ttaagaaaat
    22081 atgtgtttga tgttatagga gatgattctt ttacattagg tcatccatct ggttgtttag
    22141 cacttatatc aggatttgtt gctaaattag taggaaataa agaaaaatct acagaagata
    22201 taaacatacc tcaaagttta tcatttttta gagaaatatc tataagtggt ggaatggcag
    22261 tgtttttaat gtttatagtt gttgggatag taataccagg tgcgtttaaa gatagtaatg
    22321 agtttattgg aacgtatgca ataaagcaag gtctttcttt tggagcaggt cttataataa
    22381 tgcttcatgg ggtaagaatg ctaataaatc aaataatgcc agcatttcaa ggtatatctg
    22441 aaaaagttgt gccaaatgca ctacctgctt ttgactgtcc aatattattt aattataaac
    22501 caaatgctgt attaatggga ttcttagttg ctatgctaac atctacagct atgattttaa
    22561 tatgtaacta ctttaatgta tttaatttgc tacttatacc acttgttata acttcattct
    22621 ttgagtgtgg agcagcagca gtaataggag aatcacaagg tggtatacgt ggagctataa
    22681 taggtacagc tatagctgca atatttatgg tagttttagt aggaatatct gctgtaattt
    22741 actcaggaac tattcaaaat tggatactaa tatttggagg taatgacctt tcattatttg
    22801 gtataatcag taggtttata ggttcactat ttggaggaat attatagata tgaaatttaa
    22861 atacatagat gagataagaa gattaattga tataatagaa aacgaagaaa aagtcaatat
    22921 ggaaaaagca gtacaaatct ttgttgaagc aatagaaagt aaaaatgcta tattttcatt
    22981 tggagcttca catgcaggaa tattaacaga agaattattc tatagagcag gtggctgtgt
    23041 tcttataaat ccagtatttg caaaatctct aatgttagat acagcgccta taactcacac
    23101 aagtaagatg gaaagattag aaggatatgg tacagctcta gctgataaaa ctccaattaa
    23161 aaaaggagat gtagttatag ttcattcagt atctggtaga aatccagtaa gtatagagtt
    23221 tgctatgaaa gctaaggaaa ataaggcaat agtaatatgt ataactaatt taagttactc
    23281 aaaaagtgta tcttcaagac attcttcagg aaaaaaactt tatgaagtat gcgatttagt
    23341 gatagataat catggtcaaa agggagatgc ctgtgtagag atagatggat taaaccaaaa
    23401 agttagtcca acttctactg taatagcaac tactgtgatg aactctatta tagctcaagc
    23461 tactcaggaa ttagttaata aagggctaaa gaatcctccg attttctata gtgcaaatat
    23521 tgatggtgga gatgagttaa ataagaagat ttttgatgaa tataaaagtg taattcatta
    23581 tgaatattaa ggttaaaaaa atatgttaat gaactttgaa gtttatcaac aaagaatatt
    23641 aataatatat aataaatgct atcatttaaa agctgtaggt aaaaatctac agctttttat
    23701 tattgtagat taaaaagatg cgtaattaaa ttttaaattg gagaaaataa attggaaggt
    23761 atcaattata aatattagaa acgttgtcat agaaaaaaac tatattaaaa aaaagtacat
    23821 agaaaaaact gattagacaa taaatacatg ctttgataat attttaaatg gagtgaaaat
    23881 gtgtagaatt ataatgaaaa aagaaaataa aaatgaaaaa agaaaataaa atggagggaa
    23941 taaaagtggg tatgttaaaa aaattatgtt cactagctat ggtagcaatt atgactacaa
    24001 cacttataac agggtgttca agtggaaatg gaaaagattc aaaaaaggat ggcgaaaaag
    24061 aaaaactagt tctttggatg ccaccagtag aaaataatat gaaggaagtt tgggacccta
    24121 ttttagataa atttgaggaa aaaaataact gtgaagtaga tttacagata attccttggg
    24181 ataattattc tgaaaaattt gcaacagcta taagtgctgg cgaaggtcca gatgtaggat
    24241 atatgtatgc agaaatgtat ccacagttta tagaaagtgg agcagtagaa gatttgacta
    24301 attatgcaac taaagaggat aaagagcaat ctatttatat aaaaacatct gaaatgatgg
    24361 gtggaatgta tggtatgcca ttccaagcag caaatccagg ggtattatac tataacaagg
    24421 atattttaga gaaattagga gaaaaacctc ctaaaacatg ggaagatttt aaaagaattt
    24481 gtaaaaaagc aacacaagat actgatggtg atggaaagat agaccaatgg ggattagctc
    24541 agggatgggg agctaaaact tttggtaact taaactggaa ctggtatcct tatttatggc
    24601 aagctggtgg agatatcttt aatgatgatt taaaatcggt taaatttaat gataagagtg
    24661 gtttagaagc agctaatttc ttaaaagaat tacaagcata tgtgcctgaa gattctttat
    24721 ctaaagatag taatgaaatg atagaaagtg tatttggacc agggaaagca gcatttacaa
    24781 taatgttatc ttctgctgca acaagtgtat ttgatgaatc attcccggat ttaaattggg
    24841 gatttgtaac tggactagaa gataagaaag cagcaacttt tggagcagta gaccatttat
    24901 ctttaatgtc atctgctaaa aataaagaac ttgcatacaa gttaatacaa catatgttaa
    24961 gtgttgaatc aatgacagaa tttcataaag caataccaag agcaccaata acaaaaggtg
    25021 aaccatatca aggtgatgaa agattcaaag aaatggtgga aaatgacaaa aatgtatata
    25081 gaccattagt agtaggacct catggagttg aaatatatga atatttatgg aaagaacttc
    25141 aaactatgat ttcaggagat aaaactccaa aacaagcgtt agatgatgct gcaaaatatt
    25201 caaatgattt attagcacaa taaaagttta aagctgtaat atgttgtaaa ggactttaca
    25261 gattaaaatt agcattgtat ttataaatac aatgctaata tttatattgg agaggataaa
    25321 atggaaaact tagttagaga gtttgaagaa aattcaaaaa gaaaaaaaac aaaattaaat
    25381 attaaaccat atatgtatat attgccattg ggaataattt tagtggcgtt ttatgtgata
    25441 ccaataataa tgtcaatata ctttagtttt acaaagtata atattattag tccagctact
    25501 tttataggtt tagaaaatta taaaaaatta tttacagatg aaacattaaa agtatctatt
    25561 ataaatacta ttaaatttac tgttgttgta gtaccttgcc aaactatatt atcattgatt
    25621 ttggctgttt ggattacagg aaaaggaaat agtaaaatag cttcatttgc taaaggtgct
    25681 atatttatac cagtattatc atctatggtt ttgataggta tggtatggag ggctttattg
    25741 aatggagaag gttctattat atatcaggta ttaggtgcct ttggaataga atcatctaaa
    25801 cttttaggag atagtaagac agcattacct acacttatgt ttatatctat gtggaaaaat
    25861 atagggtatt tcatggtaat ttatatttca gctataatga atttaccaaa acactgctat
    25921 gaagtagcga aggttgatgg ggctacaaaa cttcaagaat ttataaaaat aactgtacct
    25981 ttattgaaac caactacaat aatggttgta tttttagggt cgatatggtc attacaagta
    26041 tttgacttaa tttatactgt gacaggtgga ggtccaggta tatctactat gagtatagtt
    26101 atgcatgcat ttaacttaaa ctttaaaaac tttaattctg gatatgcaat gactgtagca
    26161 aacgtattat ttttgttaat agcagtagtt tcgattttac aaaataagct tgtaagacga
    26221 gacaattcag atttttagaa aggtaggtat aatatgaaaa cagcaagaag attattaagt
    26281 gatggtttac ttttatttat agcgtgtgca agtttggtac cttttatata catgttgata
    26341 atatcattga agataacata taactcatat agtttggata tatctttttc aacagtaacc
    26401 cttcaaaact atatagatat ttttactaaa aaaggatttg cacaatattt ttttaataca
    26461 gcaatagtat ctttttcagg tgtattatta aatttagtat ttagtacatt agcaggatat
    26521 tcttttgcga agatggattt taaaggtagt gataagttat ttttatttat gataatgact
    26581 ttaattatac cttcccaagt tacaatgata ccactatata taataatgaa acacttagga
    26641 tggataaata gttatcttgc acttattatg ccaataccaa ctgcatttgg cgtatttata
    26701 atgagacaag ctattttagg agtaccaaaa gagttattag aatcagcaaa gatagatggt
    26761 tgctcggatt ttagaatttt aatacaaata gtgttgcctt taattaaacc agcattaatt
    26821 acactagcaa tatttacttt tatgggagca tggaacgagt ttatgtggcc tcttatagct
    26881 acaactaaag atgcaatgag aacattaaca gttggattat ctactcttaa gacattccag
    26941 ataactaatt atggacagat gatggcaggg gctacaataa catttttacc tccatttatt
    27001 ttctatttga ttttacagag taaatttgtt gaaggtgtgt ctttgtctgg tataaaaggt
    27061 taaatagtct aatttaataa tatataaaga aacttatata aatatggagg aatttaaact
    27121 atggaaattt catatgtact gaaagattgc acattcaaac acaataaata ctcagttgta
    27181 gaagctgaaa cttggaaaga taaggatttg gatattgtag tagctaaagg tgaaaaattt
    27241 gcatttcaaa ttatgttaaa agcaacagaa gaatttaatt gtacaattga taaaagtagt
    27301 gctatatcat ggaaaggcct gggaaataga gtaaggttag cattaaatat gcctaatagt
    27361 ttagaaaata attttagtat aaatatatta ggatatgttc aagatgatac aaaggcattt
    27421 gtgaatgatg ctattttaag ggataaagat gtgttagtag agcaatattt gcctcaaaca
    27481 ttttggattg aaggtcaagt accagaagat tttgaagaaa ataaccttga tataggtatt
    27541 gatatattta aaagctttgg atatgaagac gaagaaaaag tttgtacaat tgatgttcct
    27601 gtaaaagtaa gaaatgttgt gctaaagcct ttagatgaaa gcaagttctt tttagattta
    27661 tggcaacatc caagctgttt agctagaatg tataaagttg agttatggtc tgatatacat
    27721 tttgaaatag tcgataacta tttaaaagaa ttagcatctt taggagaaaa agttgcaaca
    27781 gttatagttt cagattatcc atgggcaggg caaagttgtt ataaagttta taaaaatcca
    27841 tcaaacttat atgaatataa catggtaagt gtatcaaaag gattggatgg aaagattaaa
    27901 tgtaactttg agagtatgga taggtatata agcatagctg ataagtataa aatggctaag
    27961 gagatagatt tatttggtct attaggtaat tggtgtgctg gtgagtttgg aaatcctgta
    28021 gaaggttata aggaccctat aagagtaaga tattttgatg aaaaggataa agtatttaaa
    28081 tttataaaca atacaaatga tttaaaagaa tatataggat tagtattaaa tcatttaata
    28141 gagtgtggat tatgggacag agtaaggatt atagctgatg aaccaaataa tccagaagta
    28201 gtcaaagaat gtatagaatt tataaattct actgtaggta ctcatcaagt taaatataaa
    28261 tcagcaactc acgaccaaaa ctttttagac agagctaaag atgaaattga tgatatgtca
    28321 ataaatttaa aacttactat acaaaattat aaagatatag aaagtcttaa gaaaaaaatc
    28381 aatgacaagg gtgggatatt gacttggttt gtatgctgtt ttccagagaa accaaatagt
    28441 tttttaagtt ctccttttgt agaaaataga ataataggat ggtatactta ttactttggt
    28501 cttgatggat ttttaagatg ggactataat ttatggacag aagacccttg gaaagattca
    28561 agttacaaat tccctatttg gaaagctgga gatatgttct ttgtttatcc tggaaaagat
    28621 ttaaagccag ttagaagtgt cagaatggag aatttaagat ttggaataca ggactttgag
    28681 ttatttacaa tgttagaaaa agaaaaggga agagagtata tagaagttga gcttatgcaa
    28741 gaattattaa ataaaaaaga aaatgctgaa attaaaggtt ttggtgacat agaactagga
    28801 tattcacttg ataattgggg atatgataaa gttaaaaaat atgtattaga gttgttagac
    28861 agagtataaa aaatataaga gtgtctcaaa atgaactttt tagttcatga ggcactcttt
    28921 ttgtatgaaa tcaatatagt ttcattgttt caacaatgaa aaataggatt atttctattc
    28981 taagacagcc ctttagtttt ttagttgtat tcaatgtttt aaaactaact ataaacgcta
    29041 taaagtttat aaatgacaat aattatatat aacatgatgt actttaattt gctataatag
    29101 ggattgaata atattatatg gaagataaca ttgtttgggg ggattaatat aaaaagaata
    29161 ggtattatta taatgttttt tttaagtata tcttttattg gattttatct tgtaataaga
    29221 gataacagtg atacagttaa gatgcaacga gggataaata taggtaatgc actagaatca
    29281 cctaaggatt ttccgtggga tgtaaaaatg tcaaataagt tttttgatga tataaaagat
    29341 gcaggttttg acacagtacg tataccagta aggttttctg attatacgtc agattctgat
    29401 aattttaaaa ttgatgaaga attttttaaa aagattgata aatatgtaga ttatgcattg
    29461 gataaagact taatagttgt gttggattta caccattttg aagaaattat gaaagaacca
    29521 agagttcata aagaaaagtt tttaaaaata tggcaacaga tagctaatag gtatcaaaaa
    29581 tatgataaaa aattggtatt tgaattatta aatgaaccta aagaaaattt atattcacag
    29641 ttactaaacg aatacattga agaagcaata aagataatta gaaaaactaa ccctaaaaga
    29701 actattatag taggaccata taatttctat caaattgatt atttgaatga attaaatatt
    29761 cctaaagatt ctaatatagt agtttccttt cactattacg agccaaatga ttttgcattt
    29821 caaggaaata tatatcataa gggatttgag cacttaagca atattacttg ggaaggaaca
    29881 aacgaacaaa tggattattt aaaaaaaagg tttgatactg tggaaaattg ggcaaataaa
    29941 aataaggtaa aaatcttctt gggtgaattt ggaataacaa aagaggcacc agaagcttca
    30001 agaagagcat ggataaaagc tgtaagagaa gaagctgaaa aacgaaactt ttcatgggct
    30061 tattgggagc ttgcatctgg atttggtata tacaatcaaa ttgaagggac ttgggataga
    30121 gatatattaa gtgcattaat agaaaagagg tgattttatg aagaaaaatt caaaaaagat
    30181 acttataact ttaactatta ttactaatgt catatatata ttgtggagaa ttttttacac
    30241 agtacctaaa gaagaaggga agtttgcctt aatatgtgcc ataatacttc tttttgtaga
    30301 gataatgggc atgatggaaa tgtttgtgca ttattatggt atgtcaaata ttgagtaccc
    30361 tgaaaaacca atcataagtg aagaacttta tccacatgta gatgtattca ttgcaacata
    30421 taatgaatct gttgatttgg tgagaaaaac tgtaaatggg tgtattcata tgcagtatcc
    30481 agataagaaa aaggtacata tatacatatg tgatgatgga aatcgtgaag aaatgtgtat
    30541 tctagcagaa aaaatgggtg taaattacat aactagaact gaaagagaag gagcaaaagc
    30601 tggaaattta aataatgcaa tgcaacatac aaattcccct ttgattgcta catttgatgc
    30661 agatatgata ccaatgcatg actttttaat tgctacagta ccatactttt tgaaaaatga
    30721 gcaagctaaa aaagatggag aaaaagagga gtatgaaaag gttggttttg tgcaaactcc
    30781 acagagtttt tataatccag acttgtttca atttaatctg cactctgaag gaaggattcc
    30841 aaatgagcaa gattattttt acagagacat tcaattggct agaaatagga caaattctgt
    30901 aatttatggt ggtagtaata cagttatttc aagggaagca cttgaagaag ttgatggatt
    30961 ttatacttat tctattacag aagattttgc aacaggtatc ttaattcaaa gtaaggggta
    31021 tagatgttat gctattccag aagtacatgc atcaggttta tctcctacag atttaaaaag
    31081 tcttattaag caacgtgaaa gatgggcacg tggatgtata caaactggta gacgtctaaa
    31141 tattttgttt aggagaggtt taggtttttg gcagaaaata agttatattt cttcaattac
    31201 ttattggtat gcaagtataa aacgctttgt atatataatg gcacctattt tattttctgt
    31261 gtttaatgtg attgtagtaa aatgtacgtt acttcaagta ttggtatttt ggttgccaat
    31321 gtatatttta agtagtttat ctcttaaaat attttctcaa aatataagaa atactagatg
    31381 gaccaatata tatgaaacta taatgtttca gtctttaatg ccagcagtaa tacttgaaac
    31441 gtttgcaata tctaaaaata aattttcagt aacaaataag agtaaattag aagaaaatag
    31501 aatgtataag tttttacagg gaatacctta ttttatttac atggtcttat ctatcatagg
    31561 aattttaaaa atgtttgtag caatatttaa aatgagttca atgacatatt cagttgtatt
    31621 attttggttg attggaaacc tttttaacct tgttatggct acattattta tatcagggag
    31681 acagcagtta agaaagtctg agcgttatat tgctgaaatt gattttaagt taaagcaaaa
    31741 ttcttatgtt ctttcttcaa aaacaattga tatatcagaa aatgggtttg catttttgct
    31801 agaaaatcca gaatatattt ccccagaaga agagtttgag gtagagttta gagaaaaatc
    31861 tggaaatgaa atgtatatag ctaatatgaa agcaaagatt gttaatgttg tggaagtaaa
    31921 ttcaaagtgg aaatatgcag cttatattac acatattgaa gatagtgaaa tagataattg
    31981 gatgtgtatt gttcacgata gaatacctac tttaccaatg acaatatcta atcaactagg
    32041 tttctttgat gatttgcaaa ttaatgtaaa aaaacgtatt gaaaaaacta gaacattatc
    32101 acgaagaagt ccaagaatca atatgaattt tcagatggat ataaaaaata caggtaaatt
    32161 aagaatagtg aattttaatt atcaatatgt attattaaat tttgagaata aaagtattta
    32221 tcctaaagaa atagcattgg aaattaatga aaatattgtg ttagaatgtg atttgtgtga
    32281 gggtaaaatt gatgaaagag gtatcttata taaagtaaat aacattgaca gcataatgca
    32341 aaatttcttt ttaagagatg aaatgatgga ttggatatta caaaataaga aaattcttga
    32401 ttcaaagcct agtgaaaaga aggaaaaaag tatagatgag tttgaaccca tggaatatat
    32461 ttaggagaga tgaaatgaag actttaaaag tgtttttttg ttctacaaaa tatcttattt
    32521 tggtgtttat tttttttata aatactactt ttataagttc agcaacaagt gataaaattt
    32581 tagatttatc ctttaaaaag atagagactg acttaagttc taagattaca tatgaggata
    32641 caggtgtaaa aatagaaacg gatagttcta aaagcgataa ggaacgttat ttatatattt
    32701 accaaaatat aaaagaaaat tggagcatgt ataataattt ttatattgaa attcaaaata
    32761 aaaataaaag ttctcagaga ataaatttaa gcatacaaag taaaaatatg cttgaattta
    32821 gattaaaaga aggtagtgaa gtatttttag aaggtaaaaa tataatatat tcagataaaa
    32881 taaaagaagg atgcatagaa gtaccaggtg aatttgaagg caaaatttat gtgaacttta
    32941 atagccttat aaatgaggaa agtaatgttg tgctggactc aaatatgctt tcaaacattg
    33001 ttagttgggg aattacgttt attccatctg atgaagaaca taatattgtt attataaaaa
    33061 agatatcttt attgtctgaa gagaaattaa gatttttaaa taatattaaa ataattggcg
    33121 atgaagaagt acaaatacca gtattgggtc aaagtataag tcagtatgaa gtattaggtc
    33181 ttaaaagtga ttctaaaatt aagtactctc ttatgggaaa acaagataat gtaagcatat
    33241 ctcaaaaagg taaattaaca ttaagtaata aagccaaacc tggacaaatt attttacaag
    33301 ttaatgttga tgataagttt aaaattggaa agaaaataac attaactgag tcttggagtg
    33361 ttaataaaaa agataaggat ggtgttccat atacattagt atatcctgag caatcaccaa
    33421 cagtacaaga tatgaaaaaa ataaacttta tgaataacat cataacgttt gttcgaattt
    33481 tatttgtatc tctagtaata atatgctttg gaatatattt atattggaaa aaatgttcaa
    33541 aaactaagta aagagaggag gttagattgt gttattttca agtttaattt ttttatttta
    33601 ttttttacct ataactttgg ttttatatta tgtatttagg tttaaccgaa cgatacagaa
    33661 catgatattg cttgctgtta gtttattttt ttatgcttgg ggagagccaa aatttgttgt
    33721 tatcatgata gtttccataa taatgaacta tatatttggt ttactggtag atagatatag
    33781 ggaaagtaaa ataaaagtaa aagttttttt agttttgatg tgtgcataca atataggtgt
    33841 tttatttata tttaaatatc tagcttttgc gctaagaaat attagtgaac ttattagtac
    33901 tgaattgact atacctagta ttgttttacc cataggaatt tcatttttta catttcaggg
    33961 aatgtcttat gttatagatg tatatcgtag acatggagaa gtccaaaaga acccttttta
    34021 tgtgggattg tatatagcat ttttcccaca attaatagca ggtcctatag ttagatatga
    34081 gagtgtggca gaacaaattc tcaatagaaa agaaacttgg aataaatttt ctattgggac
    34141 ttgtaggttt ataacaggtc taggtaaaaa agtattaatt tcaaataaca tggctatagt
    34201 agcagactat atttacacta tgaatagtca aggagaaata gctgcatcac ttgcatggct
    34261 tggttcaata gcctatactc tgcaaatatt ctttgatttt tctgcttatt cagatatggc
    34321 tataggctta ggtcttatgt ttggttttaa atttgaagag aactttaatt atccatatat
    34381 atcaaaatca attagtgagt tttggagaag atggcacatt tctttgggaa tgtggtttaa
    34441 gagctatata tactttccgc ttggaggttc tagagtagca aataaagata ttatgattag
    34501 aaatatgttt atagtgtggt tatttactgg aatatggcat ggagcagaat ggacatttgt
    34561 aatttggggg attttaaact ttgttttcct aattatagag cgatttataa tgtttgaaaa
    34621 gattgaaaat cacaatttta taaaacatat ttatgcttta ttagtagtta attttgggtg
    34681 ggtattattt aggtcaccta atttaaaaga agcatataat tattttaaag ctatgtttgg
    34741 aggcaatgga gctatatgga gtgattatac atatatgttt ttaaaagaat actgggtttt
    34801 ctttatattt gcatttattt ttagtgtacc tattgcaaag aaaatcaata agtttgttgt
    34861 tgaacaagct aaatatagta tgatatttaa cgtattttat ccaatctcag ctattttatt
    34921 gttttttatt tctgtgacat atttagtaac tggaagctat aatccattta tttattttaa
    34981 tttttagttg ggaggaaaag tatgaataga ttttttaaag taagattaat aactgcaatt
    35041 atgttcttaa ttattttatt cgctttctct atctttaatg tttgttattc tggaaaagat
    35101 acgatacagg aaataaaaaa ttcaatagct gaaaaaagta gtttaaaaga tacaatatca
    35161 tctattgata atacagtgaa tgacaaagta atatacaaaa atacgttcat tgaaacttat
    35221 ggctatttac aaaaattaat gggtaaaaat gagttttcaa aatttagtgt tgtaaaagat
    35281 acttctggaa agatgcatta tacatatttt gctaaagctc caaatccagt tggtgtgata
    35341 gcagataggg taagaaaatt ttctgatgaa atggaaaagc aaaacactaa attggtttac
    35401 ctaatgactc cagataagta tataaaagga gtgacaaaat ttccaaaagg aataccttat
    35461 tcttatagta atgagacagc agataatttt ttaaatcaac taaaaaagta taatgtggac
    35521 tatattgatt ttagagaaaa tattttaaag agtggtattc caaaagatga tttatttttt
    35581 aaaacagacc atcactggaa agtagaaact tctttttggg catttggaga attagttgag
    35641 caacttaata aaaaatacaa tatgaattta gatgaaaatc attattatag agataaggac
    35701 aattataatt caattgtata tcctaagtca tttcttggtt ctatgggaag aaagactggt
    35761 atcttatatg gtggtataga tgattttaca ttaatttatc ctaaatttaa gactaattat
    35821 acttattata cagattctaa atctcaaaaa tttgaattaa aaggaaggtt tgaagagagt
    35881 ttaatattat catatccttt taatgcagac cttgatttaa tggatggtca atcagataag
    35941 tattttacat atttattggg gaacagacca ttagttaaaa taaaaaatat agaaaatcca
    36001 gatggattaa aagtgctgtt tgttaaagat tctctaattg taccaacagc agcatttttt
    36061 tcctctgttt gttcatctat agatatgata gaccctagat attatgatgg agatatacta
    36121 gaatatgcaa aatcccatga ctatgacttt gtttttatat ctgtatatcc ccaaaatctt
    36181 acaaaagaat tttttccgtt ttgtgagtaa atttctaaaa aagttttctg ataaaataaa
    36241 tggctaactg gtaaaaagtt agccatttat tttgagtgtt attctaagaa atattttttt
    36301 tactaaatac ttctttatct atttttccaa gtgattttcc tacagtaatt gatgatacca
    36361 tagcaccatt aacatttatc atagtacgtc ccatatctag tatagggtct attgcaagta
    36421 taccaccaac taatggaaaa taagaaccaa gaccaactcc agagataaca actgaaacgg
    36481 ccattgtagc agttcctgga agaccagcta ttccaaacga actaattgtt ataacaatta
    36541 acaacataac ataaaagctc atatccatgt ttactccagc catattggca agtgttacag
    36601 ccattaaagc aggatatata cctgcacaac cattcatacc catatttgca cctaaacttg
    36661 ctgtaaagct tgatacaccc tcatcaactc cgtgattttg tactagactt tctatcgtta
    36721 caggcaatgt tcctaaacta gaacgtgaag taaatgccag aagtaaagct tcagttgaat
    36781 tttttatata agtaatggga tttagaccat ttaaagttat aaaaattaaa tgtataataa
    36841 ataatattgc aactgatata taaagagcta atataaactg taaaacagat actattgaac
    36901 taataccttg tgaagttatt gtatttgcaa gcagagcaac cacagcataa ggcataaatt
    36961 taataacagt catagacaca cttacaatta ttttgtaaga tgcttctata aaacctataa
    37021 atggttttat tgtttcaaaa tgttttttac ttaatctctt tattgaggta gctataaatg
    37081 tagcaaatat tattgtagct acaatatttc catttgccat agccattaca ggattagatg
    37141 gtagtaatcc tcttaaagta tccacaagag agtttatttc tttaagttct tgattttcaa
    37201 ctaaagtagg catattagct cctacaccta acttcattat atttgcaacg ataattccaa
    37261 caattgcagc tatagcagta gtagcaagaa gcatacctaa agatttaaat gtcagagttc
    37321 caagattgtt atctttcata tttataatta cttttataat ggataagaaa actaaaggta
    37381 ctactaacat ttttaataag tccataaatc cagaaccaaa taggctatac cacttactta
    37441 cttcttgtag ccaaatgact tgagttgtat cattaggaaa accagcaaaa gcttgtatta
    37501 tgattcccaa tatgagtcct agtactgtag catatattgt tctcaatgaa aattttattt
    37561 ttttcttttc aaattgtcta acaataaaga acgtacctag aagtaatatt atgaaaaaaa
    37621 ttgttttaaa atctgtaatc ataagaaatt gtgataaaaa tgaactttcc aatatttttc
    37681 tccttttatt taaaaataaa tttttctaag tttaaatgat atcaatatat taaaaaaatg
    37741 tcaatattat atcacaattt acttaaatag agtaaaacaa gttgattttg aaaaatgtga
    37801 ttaagtacag aaaaataggg tataataaat gtttaaagaa tttgaattgt ggtaagaaaa
    37861 taaaaaatag cttttataag atttgatttc aaattaaaat gtgttaaata aataactaaa
    37921 aattattata aaaaatgttt tcatagaata tatatcaaat gaaaagctta aaataagtga
    37981 agaaattaac tagacatcaa aggagagata aaatgagagt aataataatt ggtggtgttg
    38041 cagctggtat gagtgctgca gcaaaattaa aaagaataaa acctgagtat gaagttgttg
    38101 tttatgaaaa aactgaaata gtatcttttg gtgcttgtgg attaccatac tttgttggtg
    38161 gattttttaa tgatgctgat gaattattag caagaacacc agagaaactt agagaagctg
    38221 gaattgattt aaatatattt agagaagtcg ttgaggttga tagtgaatct aagaaaataa
    38281 aagtaaaaaa cataaaaaca gatgaaatat atgaagatta ctatgataaa ttgatgatag
    38341 caactggagc aagaagtatt atgcctccta taaaaaacat aaaattaaag aatgtttcta
    38401 cattaaaaag tttatatgat ggagaatatt taaaaaaact tttaagtaat gaagataata
    38461 aaagagtgac aataatagga gctggtttta tagggttaga agcagttgag gcatgtaaga
    38521 aattaggtaa agatgtagat gtaattcagt tagaagatag aatattgcca caagtatttg
    38581 ataaggaaat gacggatgta ttagaagaag aaataaatag acataatgtt aatttgcact
    38641 tagatgaaat ggttgtagaa ctttgtggag aagataaagt agaaaaagtt ataacaaaca
    38701 aaggggaaat agatacagat gtagtaataa ttgctactgg cgtgagacct aataccgagt
    38761 ttttgtcaaa tactaatata aaaatgctaa aaaatggagc tatagtagtt gatgagtatg
    38821 gaagaacttc tgtagaagat atatactctg ctggagattg tgcaactata aagaatatag
    38881 taagtaatga aaatgtatat gttcctttag ctactggagc taataaattg ggtaggatag
    38941 taggagaaaa cctagcagga agagaaatat cttatcaagg ttcactatct tcaagctgta
    39001 taaaaattat ggatatggag gcagcatcaa ctggaattac agaaaggcaa gctaaagatt
    39061 taggcataaa tgtcaaatct aaatttattt ctgattataa tcaaactcat tattatccag
    39121 gaagaaataa aatatatgta aagcttattt atgatgcaga tacaaaagtt attttaggcg
    39181 gacaagtggc tgggtttaag gatgctgtcc aaagagcaaa tgtattggca gctgctatct
    39241 ttggaaaaat gaccacaaat caacttggaa tgttagattt atgttatgct ccaccatttg
    39301 caagaacatg ggatgtttta aatgtagctg gaaatgtatc taaataatat ttagattata
    39361 gctaatatag ttgacaagca attaagggaa tgaatttacc taaactattt ttatcatggt
    39421 aaaattcatt cctaaatttt tattccaaat ttttattcca aatttttaga aataaggatt
    39481 ccattaaatt attcatcaaa atttaaacgc aagcttaatc tatcttctcc aagaatagta
    39541 ttttcaaaaa cattagtagc attttctaaa tacatactag gtatgtctaa agaaggacta
    39601 aaatgagtca gtaaaagttg tttgacattt ccaagttttg caagattagc agcttctcta
    39661 aaagtcatat gtttgtttct aacagctttt gatatatcta aatcatcacc atacatggct
    39721 tcacatataa ataaatcact atcttttatg aaatctggta ttgattctat aggtcttgta
    39781 tcggttgtaa gacttatctt aactcccttt ctcaattctc ctaagaccat atctggagtg
    39841 tattgttttg aatctaaaac tatattttga ccttcttgga gtttgttcca taatatttta
    39901 ggaacttcgt ttttagttgc cttatctata tcaaatttag ggtttcttct aaaattaaaa
    39961 ctgtatccta aacactctat agagtgttct aaactgattg ttgaaatttc taaatttctt
    40021 aaaactttat tatctaaaga aaaattgcct tgagggtttt ctataatttt taaagtgtat
    40081 ggtacatatt ctactaaatt tctcatagaa cgtatacaat cgactattcc aactggacca
    40141 actatagtta aatcacttgt acgaccacta ttcccaattg ttgataaaag tcctaaaagt
    40201 ccaaatatat ggtcaccatg taaatgggtt atacaaatca agtctatatc tttaaagcca
    40261 cattttttta gcttcataga tacctgagta ccttcaccac agtcaattag tatttttcta
    40321 cctttatagt ttataaatac agaggataga aatctgtttg gcataggtac atttccacca
    40381 catccaagta aagttaaatc tatcattttt tcccctttct aaaattaaca caaatctgtt
    40441 gtttgttttt attatgatat cctattataa ttattttaac attaaatata acaataaatg
    40501 tgtatttgag tcaagaaata ttgtcaaata aagtttttat atagaaatgt ataaaaattt
    40561 ggaaaacaaa aagtgaaaat tatatttagg agcatttaaa ggaggaaaat ttaatgaaag
    40621 tattaaagaa aattactatt ttgtcattga ttacattaac cttagttggt tgtagtaacg
    40681 gagaagataa aaaagtggtt gaaggttcta agaaaattca aaattctaaa aaagatgaac
    40741 agcctataat agtacataaa gatgcaaata tattgaaaga taggtatgag ccaccaaaag
    40801 gatttaaaag agttgatgta gaaaaaggta gttttgaatc atttcttagg aatactaagc
    40861 taaaaaatta tggagagaaa gtaaagtatt atgatggaag aactaaggaa tcaaaaggta
    40921 tttatgatag tgtatttgat gttgatattg gcaatagaga tttacatcaa tgtgcagatg
    40981 cgattatgct tatgagagca gaatacttgt atcagaataa actatatgat gaaataagtt
    41041 ttgattttgt tgatggtttt aaggcgaagt attcaaaatg ggcacaagga tatagaattt
    41101 cagtaaaaga tagtggttca agctggtata aagcaactga atattcaaca tcttatgaga
    41161 gttttagaaa gtttatggat atagtgtttg catattcagg aacgttatct cttgaaaaag
    41221 aattggagcc tgtaaaagtt gaggatatga aaataggaga tgtgtttata gttggtggaa
    41281 gtccaggaca tgcaagtata attatggata tggcagaaaa tgaaaagact ggtgaaaagg
    41341 tatttatgat agcccaaagc tatatgcctg cacaacaaac gcaacttctt attaatcgta
    41401 aaaatccaaa tttgagtcct tggtatagtt tagattttga aggtgatttg acaatacctc
    41461 aatggacttt taaaagagac cagttgaaaa gattttaaaa taataatttt atataataaa
    41521 agaaaaatat agatgtgaaa aaatgtaatt agattaaaac taattggata tggacaaaat
    41581 taaattgata gataaatatt aaaaattaca ctaattaatc ttactatagt ttaatattta
    41641 tctaaaaagt tttatgtaaa atataaattt tatattaaag ctaaaactaa tttgaggagg
    41701 ctaatttata ttgaagatta gaaggtattt aattttggta attataattc taattacaac
    41761 aagtcagagt tttatttctt atgggctaga gcgatttgaa gaaataacca tttttcacac
    41821 taatgatata catggaagat atgctgaagg tgatgaccat atacaaatag gaaatctagc
    41881 cacattgaaa aaagaaactc caaactcaat tttagtagat gcaggagatt gtttgcatgg
    41941 acttccaata gtaaatatgg atagagggaa aagtgcaatt gaattaatta aatgtgctgg
    42001 atatgactat attactcctg gaaatcatga ctttaattat ggaaaagata gactgtttga
    42061 gttatctaaa agtgctaatt taggagagaa tactttaaaa tttttatcat caaatatttt
    42121 tgaaaatgga aaaaaagttt ttgatagtaa tgatataaaa gaaatagatg gaataaaaat
    42181 aggatttttt ggactctcaa cccaggaaac aaagagcaaa actggattta aaaatgtgga
    42241 aggattagag tttaaaaatc ctatagaaag tgcaacagaa caagtaaatg aattaaaagg
    42301 aaagggtgcc aatgtaatag ttgcaatatg tcatattggt acaaatagtt taagtaagcc
    42361 aaatagtata gatatagcaa atgaggttca aggaattgat ttgattattg atggacacag
    42421 ccacactaag tttgaaaatg gtaaacaagt gggtgataca ttgattgtaa gtgcaggaca
    42481 atatttagaa aatattggcg agataaaact aactttggat actaatgatg tagaaaatat
    42541 aaaaatagaa aataaatcag ttagattaat tggaaaagaa gaagcattaa agtataagct
    42601 tgactccgct gtaagtaaaa aggttgatga aataaaagaa caacaagaaa aaatattaga
    42661 taaagttata ggggtaacta caaatactct agatggttca tatgaaaatg taaggacaaa
    42721 agaaactaat ttgggtaatt taataagtga cattttacta gataaaacta aggctgatat
    42781 ttctttattt aatggaggga atattcgaga tacgatagaa aaaggcaata taacaagaag
    42841 agatattgta gatgtatttc cttttaacaa tactatagtt accaaagaac ttacaggagc
    42901 acagattaaa gatgtattgg agcatggagt aaaactttac ccagaaaaaa gttcagcctt
    42961 tttgcaagtg ggaggaattt catattactt taatcctaaa caaaaagaag gcgaaaaaat
    43021 aacaaatatt caaaaagaag gaaagccatt agatttaaat aaaaagtatg tagttgcaac
    43081 gaatgattat atagcatcag gtggagatga atttccttgt ttttcaaaag agccagtact
    43141 aaaggaattt ggaaatttag agagtgcagt cattgaatat atagagtaca aaacagaaat
    43201 atcaaaaaat gtagatggaa gaattggtat taaatctaaa gatgagttaa taagcaagaa
    43261 tcatgagagt gatttgatat taagtgaaaa tgaaaaaaat atcaatgata atgaagagaa
    43321 tctatctaat aatataaaaa gctctgatag tgataaaaat aaaagcttag ataaaacaag
    43381 tgctagagaa agttctaatc taggaatatc taaaagagaa gctaaagaaa aatctcctaa
    43441 aacaggagat ttaggttttt caaatagtat aattatattt atagtttcaa gtacattgat
    43501 atgtttattg aattttaatc aaaaagagtt aaaggataaa aagtcaaaat aaaaattaat
    43561 gagcaaaaac tagtagatag taatttaatt ttagataata gtaaacagca aaggaaatta
    43621 gttatataaa tactaatttc ctttgtttta tgttttaaca tgattacttt taaataaaat
    43681 tgtattttat gaaaaataat tacactaaat ggcatagtat tatggggatt tttagttttt
    43741 attcaagtaa atgaaatcat attaacaact tctgatatat ttacaaattg ataaaaaaat
    43801 gattttatta gtatgatata atttagctag tatatatgat gatggaggat atagggatga
    43861 ataatatatt acttttggaa gatgacaaat ctttaaatag aggaattagt tttaaattaa
    43921 aaaaagaagg atacaatgtt tttagtgcat ttggtataga agaagcaaaa aatattttcg
    43981 caaaagaaga gatatgctta attatttccg atataggtct acctgatggt agtggttttg
    44041 acttttgtga ggagattaga aagaaaagtg atgtttacat aataatgttg acagctcttg
    44101 atgaagaggt ggatattgtc acaggatatg atttaggtgc agatgattat atcacaaagc
    44161 cttttagctt gatggtatta atatcaaaag tgaatgcgct tatgaaaaga gtaaatacag
    44221 taaaaaatta tactctatta gtttgtgatg atttattctt ttattatata gaaaataaat
    44281 taattataag aacagatgat aaggaagaag agataatttt aagtaagaca gagacaaaac
    44341 tattaaaata tttgatggaa aattctatgc agacattgac taaagagcaa ctgttagaat
    44401 ctttatggga tagtagtggt aattttgtag atgataatac gattgcagta aatataagaa
    44461 gacttagaca aaaggttgaa aaaaatccat ctgaacctaa atatataaaa acagtaagag
    44521 gggttggata tatgtgggga gaaaggagta taaaaaaatg ctaaaaaata tgaacctcaa
    44581 agatggttta agtgtgaaag atgattatat aatgagaaga gttattgtat attctgtttt
    44641 atctgtacta atagtaaccc ttgtatttat actttttgaa tatagcagat tacataaatt
    44701 gtatgaacat caaataaaaa atactcaaaa tattgctgga atattgattg aaaaatatcc
    44761 taatgatgaa atagatatag taaattctat ttataattct gattatagta aggtgtctac
    44821 tggagaaaag gcatttaaga aatttggcta tggattggaa aataaaatgt caaatgataa
    44881 aaattttagc ttatacttaa aatctttttt taaggaatca ttttttattt ttttaatgat
    44941 gtttatagta gttataggta taatatatta ttttataaga tacataaata aaagattgag
    45001 taaaatttat tttatagtag ataagatgtc acaagaaaac tacttgaagg acactactga
    45061 gtcagaagaa actggttatc tagtagaata taatgaatac tttaaagaag gcatatttag
    45121 taagataaat aactgtctat atgagcttaa tagaagttta aagataaagt ttattaaatt
    45181 agaaaaagaa aaagaaagtg taaaatcatt agtaacagat atttcccatc aattaaaaac
    45241 tcctttagca tccttaaaat tatacaatac attacttata gaagaagaat tagaagaaga
    45301 tgaagccaac gagttcttat tgaccaataa ggcttctata aataaattag aaaatttaat
    45361 aaattcttta gtgaacatat caaggttaga aattagcatg ataaacataa aaaaagaaga
    45421 taatgatata aaaaatacta ttctaaatgc agtaaaaagt gttaccccta aggctaagag
    45481 caaaagtata acattaaatt taaatgaatt taacagtaga attataccac atgataaaaa
    45541 gtggactgag gagagtattt ttaatgtatt agacaatgct gtaaagtata cacaggtaaa
    45601 tggagaaata aatatatctg tagaagaaac tgctaatttc tttaaaatta taatagagga
    45661 taatggcata gggattgaaa aaaatgagtt taataatata tttaaacgtt tttatagagg
    45721 ttctgctgat gaaattgaat ctgtagaagg tagtggggta ggattgtatt taagtagaaa
    45781 gattttagaa gaacaaggtg gaaatattat agtttcttca aaagtaggaa tagggagcaa
    45841 gttttctttg tttcttacaa ctatgtaata ttaaatgaaa ggaaaatgta agcctctaca
    45901 tttattatat aagtataaac agctccagaa ataaaaaatt aataaaatat ggggtaatat
    45961 aacttagaat gagaggataa ttttatgaaa atactatata ctgaaaattt atcaaagcat
    46021 tatggaaaag gagaaagctt agtaagagct ttggacaatg ttgatttaga aataaatgaa
    46081 ggtgaatttg ttgcaataat aggaaaatca ggttctggaa aaagtacact tttgcatatg
    46141 attggaggtc ttgatatacc tacatctggg aaggtgtata tagataataa aaatatattt
    46201 acattaaaag aagaagaact tgctgtattt agaagaagaa aaatagggtt tatatttcaa
    46261 agctataatt taattccatc tcttaatgta tgggaaaatg tagtgttgcc aatcggttta
    46321 gatggtagag aggtagatga atcttttata aaagaacttt tgaaatcatt aggacttgaa
    46381 aataaacacg acgttttacc aaatacatta tcaggtggtc aacaacaaag ggtggcaata
    46441 gcaagagcat tagctacaag accagctata atacttgctg atgaaccaac tggaaaccta
    46501 gattctaaaa catctgatga ggttatgagt atattaaaat ctatgtctaa gaagtacagt
    46561 caaactctag ttatgataac tcatgatgac agtatagccc aaatggctga tagagttata
    46621 ttcatagaag atggcagggt gtcaaaggtt ggtgataaaa atgattaata ctactaaaat
    46681 agcaaaaagt aatttaaagc aaaataaatc aaaaagtatt ctaataataa ttactataat
    46741 actttcaacc ttaatgcttt cttctattgg aatttatatt gttgatgcag gagcatatca
    46801 aaaagaaaat actataaagt attcaggcaa ttatcaaggt atattagcaa atgtagatga
    46861 aaaacaggct gatatattga gtaatcatgc agatgttgaa ttaactggtg aaatgaatgg
    46921 agtaggtgtt gaaaaactgg aagatgattc aaatatttct ctagcataca tgaatgaaga
    46981 tgctttgaaa ttaaatagtt ttgaatttat caaaggaaaa ttgccatcaa aagagaatga
    47041 aattgtgtta gatagtggag ctttaaaggc acttggttat aaaaataaag atttaggtga
    47101 aaagataaaa atatcttatg atgactataa aagtgataaa aaaattgaaa aagagttcat
    47161 tataagtgga atcttaaaaa ctagcgaaat atctgaagca ggtaaatatt attatgctat
    47221 tatatcagaa tcatatatga gaaatactag agatatgtcg caagaagatt ttaatatata
    47281 tgtgaaatta aatgataaaa gtaatttatc gatagaacaa gcaaaatcaa aattaaataa
    47341 gattgctaat gatgtaggat tggacactat taatgtagct gtaaatgaaa actatataaa
    47401 tgctttaaaa ccagatatgg aaacaattat gggtggagta tttgtagggt tagtaattgt
    47461 attgtctagt attttagtaa tatacaatat attttatatt tcaatagtta caaaggttca
    47521 agaatttgga aagcttagag caattggtgc aactaaaaaa caagtaaaaa atatagtatt
    47581 taaggaaggg ttcatattag ccggaatagc aataccaata ggaataattt taggctatgt
    47641 acttgcaaat attataataa aatcatttat ggatatagat gtaaaatcat cacgattacc
    47701 agttatatta tcggttgtgg taataagctt tatatctgtt gttttatcac ttttaaaacc
    47761 aatgaaagta gcatcaaaag tatccattgt tgacgcagta agatatacag gaaataaaat
    47821 aagtaataag agtaagagaa aaggatataa aaatataaat ctaaagagat tatcacatgc
    47881 caatttagaa agaaataaaa aacgtactta catgacattg gcatcactta tacttagtgg
    47941 aactatattt ataacagtat caactgcctt agaaagtttt gatgctgaga aaatggcaag
    48001 agaacacttt ccttatgata tagaagttag actatctggt tatgaaatga acagtgataa
    48061 aaatcctaaa gataatttga atattttaca aatggataat cctctaagta aagatttttt
    48121 taatcaaata aagaacatgg aaggaattaa aagaatagaa tctgcaagaa gtgtaaaaat
    48181 tggtatggag gactatgatg ttgaatttaa gtatgacctt ttacaaagta taaatgaaaa
    48241 tgatgtaaaa tcattaaaca agaatataat agatggaaaa ataaatcttg aaagacttca
    48301 aactggagat gaaatagtaa ttactcatgt tgatacagca aaagaaatgg gagtaaaagc
    48361 aggagataaa ataagattaa ctttatatga tggagataaa aagataaaaa aagaatttaa
    48421 agttcaagca atagctatgg gggttccaag ttttggtata gggaaggatt ttattgatag
    48481 aactttgaaa tacgattcaa ctagtagctt gggaatatat acagaagaag gaaagtatca
    48541 agaaatcaaa gatagtatca agaagattgc taaaaataat ggatttttag agactgattt
    48601 tatagatagt agaattgagt caaataaggc tactatttca tttataaaga taatgggata
    48661 cacgctaaca ggcataatag gtgtgattgg atttatgaat cttgtcaaca caatgataac
    48721 tagcatagtt actagaaaaa aagaacttgg tatgcttcaa gctattggtc ttactaataa
    48781 acaattagtg aaaatgctta attcagaggc aatgtattac acttctggaa tgatgatagg
    48841 aagtattcta tttggaggta tattagggta tattgctgtt atatttctaa aaaagactgg
    48901 attatcttat gcaacatatt ctttgccaat agtaccaatc ttgttgatga ttgtgtgtat
    48961 tcttatagct caatttataa caacttattt gataggacga agttttaata aagaatctct
    49021 tattgataga gtaagataca gtgaataaaa tacaaatatt tatggttagt ttaactagca
    49081 aaaaacatat aaaaaaatat agttaaagag attcataatt aaataggtta aagcatagta
    49141 cagagcaaac aatttaagca gatgtttgct ctgtatttta ttatgtaatc aataatatat
    49201 ctaatatact gatattattc atgttagaaa atgtaccggt acaattctaa aaaagtataa
    49261 aattgtacca tataaaatga aaattttgta taataatcgt atagaaaata aataggagct
    49321 gatatatatg aataagaaga ttaattatgc accaggacca actgaaacta gggaaaatgt
    49381 aagattagca agagcagaaa aaacaacaaa tccagatata gatattgatt ttgttgaatt
    49441 ttataaaaag acatgcgaga agtttggtaa tataattgga acaaaaaacg atgtttacat
    49501 attgagtggt gaaggaatat taggtcttga agcagcatgt gcatcactta cagaaaaagg
    49561 agatagagta cttgtaattg acaatggtat atatggagaa ggttttaaag attttgtaac
    49621 aatgtatggt ggggagtatg tgcttttttc aagtgaatat actaaaagta tagatattga
    49681 tgaattgaaa gcatttttag atgaggatag taattttaaa tatgcaacag tagtgcattg
    49741 tgatactcca actggcgtat taaatgatgt aagcaaaata tgtccattac ttaaagaata
    49801 tggaatatta actgtagttg attctgtagc aggtatggta ggagaaagat taagtgtaga
    49861 tgaatctaaa atagatataa tattaggtgg ttcacaaaaa gctatttctg caccagcagg
    49921 acttactata gtcggaataa gtcaagatgc aaaaaattgt ataaaaaata gaaaaactga
    49981 tgtaataggt ttttattgta atttaaatat atgggaaggc tattatgaaa aaaaatactt
    50041 cccttataca atgccaataa gtgacataat ggggcttgat aaagcacttg ataatatatt
    50101 agaagaagga atagaaaatg tattaaatag acatgaaaag atagcttata gtgttagaaa
    50161 atctattgag gaatatggtc tagaactatt tttagaagaa ggatattcta atacagtcac
    50221 agctataaaa attcctgaaa gtataggagc tttagaactt actgattata tgcttaaaaa
    50281 ttataataca cttgttgcaa catcactaaa tcaatatatg gatactattt taagaatagg
    50341 tcatatggga gagaatgcaa atctaaataa aatagaacat gtactaaatg ttttggataa
    50401 aagtttaagt gcattaggat tcaaaggaaa tggaaatttg ttaaatttat ttaataagta
    50461 ttatttttaa tagatagggg gatatagtat ggaagttaca gctaagcaaa aatttacaac
    50521 gaaagacatg gtggaaacct cactattaat agcattagta tttattgcaa ctaaatttat
    50581 aaacataaga ttaccaatat ctataaatgg tgggcttgtg catttaggaa ctgcaatgtt
    50641 gttcatatca gcaatagtat ttggaagtaa aaaaggtgct ttatcaggag ctattggtat
    50701 gtcacttttt gatttgatat ctgggtggac tttatgggca ccgtttacct ttatagatag
    50761 aggagttatg ggttatttac ttggaaagat tgcatgggca aatggtaata atgggaataa
    50821 ttttttaatc aatgtaatag gaatatgtgt ttcatcaatt tggatgctat ttggatatta
    50881 tgtaacagaa gttatacttt atggtaattt tatagtacct cttacttcaa taccaggtaa
    50941 tttaatgcaa gtattgatag gtcttataat agcgcttcct atttctaagg tattgaaaaa
    51001 atgtataaaa taaatataaa gtgaactaaa atttatgata gcataaatta tactgtataa
    51061 tattaggatg gagtacataa ttaaaaaatt ttagtataga ggttagttca caatgttatc
    51121 agttacaaga tattcaatca aaaatgttcg tttaaagccg tgggtaaagt ttatttggta
    51181 ttttgagaca aaatcaaata tactgttaaa taataagtta cttccgacag acagtataga
    51241 tattattctc aatctttctg atgttatgga atataaaatt gaaaatcaag actatattgc
    51301 tagtaatatt cattttaatg gaataaggga taaacatgga tttattattc aacatggaaa
    51361 tatacgtgtt atagggattt ctttttatcc atttggtctc tatccatttt taaagatacc
    51421 aatttcagag tttaacagac aaattgttga cctagaagca gtatcaaaac tatttgcaaa
    51481 aaaattagaa gagtcattaa atccaacaaa agcaatagaa aaagttgttt tatgtttaga
    51541 agaagtatta ttatccatat tagaagaaga tttaatttca aataaatatg taaagcttct
    51601 aaattcattt atatatgtta atagatatag caatataaaa gcattttgtg atgatacaaa
    51661 tataaatatt aaaacattgg aaagaatctg tttaaaatat acagggtata cacctaagat
    51721 tttaaagaga atttacagat ttaaaatggc aagcaatcaa cttatttata attataaaga
    51781 tgatgaactt tttgatttta tttatgaaaa tgagtactat gaccaagcac actttataaa
    51841 agaatttaaa aaattttcag ggacgtcacc aattaaattt agtggtgaga ataagacgat
    51901 taaagaaaat ataaagtaca gctatttata attatactgt cgatttttta caatacctcc
    51961 aatttatttt ttgatatgat ataggaaaat aaattggagg ttttacatat gaaaattatt
    52021 acagaaatag ttgagtttaa tgtggaagaa aatttatcaa aagaagagtt tatagaaatt
    52081 gtaaatgact tagaattaaa atttcattct attcaatctg gttttattga tactgaactt
    52141 ttatatgatg aaaagtctca aaagtggatt atgatacaac attgggaatc tatagaagac
    52201 ctcaagagtg tttctagtaa aatgtttaaa gattcttcaa ctgagaaatt tagaaaagct
    52261 ttaaagaatc aaactgttaa gatgaatata ataccacaaa ttaaaagttg gaaattatag
    52321 aactgaatgt aaaagatagt taaatagtat caaactaaat tagccaaata acattttgta
    52381 tttaaattgt tatttggctt ttagtgttta ttttaatatg agtgttatat actagaaaaa
    52441 ccaacaaact gttagagtgt ggattatcat aatgtcatat tcgggagcta aaaggcttaa
    52501 tactgatttg ccaattggtc aatccatgca tgtccatctg gatatgctgt tattaaagca
    52561 tttacaagcc aatccctttg ctcttgtgat aatagtggga ctgttacatc gaagtctttt
    52621 tgataaccaa gcctatctat gtatattgat ttgcaaaaca aaaccacttc gggagccata
    52681 tatgggatat tatctgatgt tatcagtctg gccttatcca tatctcttat aattctttga
    52741 ttttgggaaa aaagaaattt tccatctttc tgtggattat atatcaattc aataaaatcg
    52801 aactgtaact gttcgtcaca ctgtatattc attttatagt tatcatcctc gacttttttt
    52861 aattcaataa atgagcaatc aggcttaatc gcaaaaacac aaatttgctc tatatgttgc
    52921 ttgttaaggt cgataatagg tcgtaataaa taattaccca agggctcata tatatcccat
    52981 ccttgattaa gcatatactg aatgctcttt tctttatctg tgtcagaaat ccatatgtca
    53041 atatcactgt gtgggcgaat atgcatattc gcaaataatt ctagggcata tccaccacat
    53101 attgaatatg aaaagccaca atcattaaaa aagtttacac aatctttaat tagtttgttc
    53161 atagcaacct ccttttgcat gtactgaaat aaattaaatt ttttaaaata ccaactaaat
    53221 gtttgttttg tgtaaatata aaactgccga tattaaggca gttttatatt tactatttag
    53281 aaaatttatt aacaaatttt tgatattcac acattatctt atcattcata aaagaaatac
    53341 ccataaaagc agcttgctca ccaaacaaag taaaacgttc tatcatctgt tgtggattac
    53401 ctggataaac tgttggaata caccatgtgt ttagaagaaa accaaacatt tgagcagcaa
    53461 tctctggatt tttaacttcc atagaattat cttgatttcc ttcttcaatc aatgctttta
    53521 tccaaggcac aatatcattt ataatctcat caaactgcgt agctaaatac ttaggattac
    53581 gcaatagtga tgatgacatt aaaaaaatgt cttgtttatt gtgctgttca taagcatgaa
    53641 gcaacctata ttgtaatttt tctaatgcgg tctttccttc atcatttttt acctcatacc
    53701 cataaaatcc atcattatac aaccgacttg tgattgcctt aattatttcg tcttttgatt
    53761 taaagtgatg ataaatagca cctcttgaaa ggccatctaa attatctaca atgtcttgaa
    53821 tcgttgtctg ctcataccct ttttctaaga ataacttatg tgaaatatca agtatttttt
    53881 gtatagtatt atcaggttct ttttttcttc ccaaaaaact cactcctcga aacaatcatt
    53941 tggtatgttt atcttagcaa atacataatg atttgtcaat gtgtcttttt agcatgttga
    54001 acaggtatat tgccatgctt tcattatttt atttttttgc cttatactgg actatatttg
    54061 cagtttccta atatatttgg ctcatattta aataccatta tctaaaagca ctattgctgt
    54121 acgagaggca ataggcattt tagaagaagg aggagttgag ctaaagtata catcataatc
    54181 tcttaagttt tctttttttg tgtttcctcc aaactctacc atatcagtat ttaatatcgt
    54241 tttgtagttt gtattttgaa actttgaaat atcaactgat tgtattagat tagggtggaa
    54301 attaaatata aataaatatt tgcccttact gtacaccaaa atctttttat cattatccat
    54361 atgtataagt tcatgactat tttggataaa aatatcactg tattttgtaa gttcgagcat
    54421 agatttatca aaatttaata attgtttata ctttaagttg ttatcttctg aaaggctcca
    54481 ttgtcttctg gcatacttat agctccaatt gttgccttct cgtggaaaat caatccactc
    54541 aggatgtcca aattcatttc ccataaaatt tagatatcct tcacctgcta gagaaaaagt
    54601 tattaattta attaatttaa taagagatat tgctctattg ataacatggt tatttgagtt
    54661 tatttccata ttccagtaca tttccttgtc agctagccaa aaaataattg ttttatcgcc
    54721 aactaatgct tggtcatggg attcacaata tcctatattt ttttctccag gtcgtctagt
    54781 agtaagttca taccacattt tgcccaaatc ccagtcttca tcagataagt tagagatagt
    54841 ttttatccaa aaatcaggta ctcccattgc gagtctatag tcaaatccaa ttccgccgtc
    54901 ttttataggg atacacatac caggcattcc actcatatct tctgcaatac ttatcgaatt
    54961 aggtttaatt tctttcatta attcatttgc aaattggaga tatgttatag cttcaatatc
    55021 tgtattcata ctaaaatatt tttcataact atcaaatgaa actccaagac catggttatg
    55081 atataacatt gaagttacac catcaaacct aaagccatca aaatggtact catttaacca
    55141 aaattttata tttgaaagca aaaaatgtat tacttcaggt ttaccatagt taaaaagttt
    55201 tgtaccccaa gcaggatgat ttccctttga acctgagtgg aaaaattgat gttcacttcc
    55261 atcaaactca tttattcctt ctaaagtatt ctttacagca tgagaatgaa ctaaatctaa
    55321 taaaacagca atccccattg aatgagcagt gtttatgagg ttttttaagt cttcaggagt
    55381 accaaaccta gatgagattg cataaaagtt tgaaacttgg tatccaaaag atgcataata
    55441 tggatgttcc ataatagcca taagctgaat ggtattgtat ccagcttttt ttatcttagg
    55501 aagtattttt tcagtaaatt cattataagt tccaatagat tcgctttcag tagacatacc
    55561 tatatgacat tcatagatta gtggagaagt tatgtttttt aagtcaaaat cattgtcagt
    55621 ccagataaat ggagtttttg gttgccatat ttgtccatta aatccaccaa aatctttttg
    55681 aattactctt tttatatata atggtatacg gtcaaatgtt tttccatttg cagtaacttg
    55741 tacctttacc tctgatttgt gaggtaagct attttgacca ggaataaata tttcccaatt
    55801 accagaccca atttttctta gaggatgaga ttttctattc caattattaa agtcaccaat
    55861 tagtgacaaa ctatctgcat ttggagccca ttccctatag aagaatccat catttgtctg
    55921 atgaaatcca tagtaaagat gagcatttgc aaaagaacta aaatctttat agtttttcaa
    55981 tgtttttgat ttcatatcct cataatgttt cattcgcttt tttatatcag attcaaatgg
    56041 ttgtaggtat acatcaatat ctaaaatttt aaatcttttt tgacttttca ttgtatttct
    56101 cctccataat aaatattaca tctaaaaaat aatatttatc tcatactatc ttctctgtat
    56161 aattgatata tattatataa tcctaataaa aagcttaagc caccaatgat taatttcata
    56221 atttacctcc ctagtattta tacttatata tcgtattcat caaaatttaa aagtttgttt
    56281 aaaactcttt taataatatc ataatcaaag cctttatagg aaagatgttg agaaattttt
    56341 tgatatattt tctttttatc ttctttcttt actctttcat accttttttt tgctagatac
    56401 atagcatttt caaattcagt gtccttgtct aattcagata ctgcttcatt gattgtactt
    56461 ctattgatac ctttattata taaattctgc ttaattctgt ttttgccaca tttatttagg
    56521 ttaacatttg tgttaacaat tttttgagct aatacactgt catttacgag attataactt
    56581 ttaagaaaat ctatgaccct ctcaattata ttttcatcaa attctgatga tagtttttct
    56641 tttaattttt tttcagattg gtcagcatga gataaaatat ttaatgcttt atttttagct
    56701 tttatataca tttcatcatc taaaataggt tttaattctt cttcatttat ttcattccct
    56761 ttttttaaat taaatgtata aaccagttct ttaaatatag caataaaaaa tttatcatct
    56821 acataaatat taactctatc atcatttctt ttttgttgtt ctatttttgt aattataccc
    56881 ataagtcctc cttttttata tgtaattata ttaacaaaag caactaataa aatttaacaa
    56941 aaaacttata tttgtatgtt atattttatc aaaattaaga caaaatagta aaaagttatt
    57001 tttattaaaa agtaaagcag aatcatgtta aataattatt ttttaaagtg ttttttagca
    57061 tataaatata attatatacc caaaaaaaca aattataagc tttaagaaat aaaaaataat
    57121 ggtacaatag tattataaca ttaaaaggag acaaaatatg agaagtgaaa atctagttaa
    57181 gatggcagag ttgaaaaata ctgaaaaact agagaaattt aatcgccata aaggcaaaat
    57241 caaaataggt atattgggag gtacttttga ccctatacac tatgctcatt tagcaacagc
    57301 agaatttatt agagacaagt atgacattga caaaataatc tttataccat caggaaatcc
    57361 accacataaa ttatgtatca ctacagataa gtatgataga tataatatga cacttctagc
    57421 aactgaaagc aacgaagatt ttttagtttc aaaagtagaa atcgaaagaa agaaaaggac
    57481 ttatacaata gatacattaa aatacttaaa gaaaaaatac aaaaatgcag acatttattt
    57541 tataacaggt gcagatgcaa tttgtagtgt tgaagagtgg aaagatgtaa agaaaaactt
    57601 tgagttagct acttttatag ccgcaactag accaggaatt agtttgttaa ggtcacaaga
    57661 gactatagaa aaactaacta agaagtataa tgctgacatt ataacagttt atgttccatc
    57721 actggatata tcgtccacat atataaggga acagctaaat gaaggcaagt caatacgtta
    57781 cttagtaccc gaaaatgtag aaaattattt atatgaaaat aaactatatc aatatggaga
    57841 tgattaaatg aacttagaaa atattaacca aagactaaat caaatgctac cagtggggag
    57901 gcttagccat tcaaaaaatg ttgcaaaatg tgctgaaaaa ttatgtgaga tttatggatg
    57961 tgacaaagaa aaagcttact tagcaggaat gattcatgat tgtgcaaagt atttaagtga
    58021 caaggaaata gaggattatg taaataagta tgaaatatat ttagacccaa tagaggatgg
    58081 aaatcgttcc ttgtctcata gtgtaattgg ggcgtacata tgcgagtatg aatttgaagt
    58141 ggaagatgaa gatattataa atgcaatcaa atatcataca acagggagag aggacatgtc
    58201 tcttttagaa aaaataattt acatagctga tttaattgaa gaaggaagaa aattcccagt
    58261 tgtagataca ttaagagaat tagcatatgg agggaagctt gatgaagcac ttttaacttc
    58321 ttttaataat actttaatgt ttgtcatcaa taaaaaagaa gagatacatc caagaacagt
    58381 tatggcaaga aactatctaa ttaaagaaaa gttattataa aaaagctata taaaggcaaa
    58441 catattaata aatttaaatt tgactatgtt acaaaatcgt attaatatgt tacaataact
    58501 aagtgtattt taattaggaa agtgaggaat ttttatatga cagtggaaca aatgacaaaa
    58561 atagcatatg atgccataga agataaactg ggtcaagata cagttataat aaatatagga
    58621 aaagtttcta gtttgtgtga ttattttatt attactacag catcttctca gagacaggta
    58681 aaagctattg ctgacaatgt ggaagatgaa ttagcaaaac taggattaga gccaagaggt
    58741 aaagaaggac aaggaactca aacttgggta ctccttgatt atggagacat aatggttcat
    58801 gtatttaatg aggaaaatag agggttttat aatttagaaa aattatggaa agatgcacct
    58861 tatatagata ttgacacatt agcataaaca tgataatata tgttattata attagaaaat
    58921 ttcaaaaatt atattaaatt gaatagacta gagcagtaaa agattatatt ttttcagaga
    58981 gttagtggaa ggtgagaact aatattatat aactttgaac ttactagatt aaaatctatt
    59041 ctccatggct ggattaaaag ctatctaaga gagtctatat atgtagtagg ctaattaggg
    59101 tggtaccgcg aagatttatc ctcgtcccta aacgtaagtt tagtgacgag gattttttat
    59161 tttcaaagaa ttatttggaa attataagta aataatacta aataaggtag ttatacacaa
    59221 ttaaaaatag gaggcaaaat atgagcgtat ataattttaa agaagtagaa tcgaaatggc
    59281 aaaaaatctg gaaagataat aatcagtata aaatggatac tgcacaaact gaaaaaccta
    59341 attattacac attagaaatg ttcccttatc catctggaaa aatacacatg ggacatgtta
    59401 gaaattattc tataggtgat gttgtcgcaa gattcaaaaa aatggaagga tataatgtac
    59461 ttcatccaat gggatgggat tcttttggat taccagctga aaatgcagct ataaaacatg
    59521 gaatacatcc acataaatgg acaatggaaa atatagaaga aatgaaagag caattaaatt
    59581 tattaggtat tagttatgat tgggataaag aagttgcaac ttcaacgcca gaatactata
    59641 gatttacaca agaaatattt ttgaagtttt tagagcatgg gcttgcatat aagaaaaaat
    59701 catatgttaa ctggtgtcct tcttgtgaga ctgttcttgc aaatgagcaa gttgttcaag
    59761 gagcatgtga gagatgtaaa gcaacagtat taaagaaaga tttagaacaa tggtatttta
    59821 agactactga atttgcagaa gaattactta atgacttaga tatattagat ggatggccag
    59881 aaaaagttaa aacaatgcaa aaaaattgga taggtaaaag tacaggagca gatttagttt
    59941 ttgatataga tggaactgat aaatctatga cagtatttac aactagacca gacacaactt
    60001 atggagttac ttatatggta cttgctccag agcatgaatt agttaaagaa ttggttgcag
    60061 ggactgaata tgaagcagat gttgaagctt ttgttcaaaa aatgcatact atgacagaaa
    60121 tagaaagaac agcagctgat gtagaaaaag aaggaatgtt tataggtaga tatgttataa
    60181 atcctttaaa tggcaagaaa gttcctctat ggatagctaa ctatgtatta gttgagtatg
    60241 gaacaggtgc tataatggca gttccagcac atgatgaaag agatagagat tttgctgaaa
    60301 aatataattt agacataatt gatgtaataa cagaagataa caaaatgata aattcagaag
    60361 aatttaatgg attagatgct tcagaaggat ttgaaggaat aatcgataag ttagaaaaag
    60421 aaggtagagg aaagagaaca attaattata gacttagaga ctggttagtt tcaagacaaa
    60481 gatactgggg ttgtcctata cctgtggttt attgtgatga atgtggaata gttccagtta
    60541 aaaaggaaga tttaccagtt cttttaccaa cagatgttga atttactgga aaaggggaat
    60601 ctccacttac gacttcaaag caatttatgt caacaacttg tcctcactgt ggaaaaccag
    60661 ctagaaggga agttgatact atggatacat tcgttgattc ttcttggtat ttcttaagat
    60721 atgtggatag caataatgaa aatgaaccat ttagtaagga attagttaat agatggcatc
    60781 cagtagacca atatataggt ggagtagaac atgctataat gcatttactt tatgcaagat
    60841 ggtttgtaaa ggcatttaag agtatgggaa tggtagactt caatgagcca tttaaaaact
    60901 tacttactca aggaatggta cttatggatg gttctaaaat gagtaagtct aaagggaata
    60961 cagtatctcc tatggatata atagatgagt atggagcaga tactgcaaga cttttcgtat
    61021 tatttgcagc accaccagaa agagatttgg actggtcaga gcaaggtgtt gatggatgct
    61081 ttagattctt aaatagggtt tatagattgg ttgacgagtt agcagatgtg gttaaaaagg
    61141 atgtagagtt tggagaatta aattcccaag ataaggatat gagatatact atacactcta
    61201 cacttaaaaa agttactgct gatttaagtg aaaaattcgg atttaatact gctatatcag
    61261 ctcttatgga attaataaat gatatgtata aatataaaga gttagataat ataaacgaag
    61321 cagttataaa agaaggtgta caaactatag taactataat tgcaccattt gcccctcatt
    61381 taggtgaaga actatggact atgataggta aagaaggaag tgtatttgac atagactggc
    61441 ctaaatatga cgaaaaagct ttagtaaaag atgaaataga agttgttgta caagttaatg
    61501 gaaaagttag aggaaaatta actgttaact caaatatatc taaagatgag atggaaaagg
    61561 tagctttaga agatgaaaaa ataaaaggat tagttgaagg taaaactata gttaaagttg
    61621 tagctgtacc taaaaaacta gtaaatatag ttgttaaata actcaaaaat atgaacaaaa
    61681 atataaattt ataaaataaa cgaggctgtc tcaaaataga gataagcctt ttttcattgt
    61741 tgaaattata aatttgttga aattataaaa atagatttga ttttatacaa aaaaatgatt
    61801 aaagaactaa aaagttcatt cttatatacc ctagttgttg ttttaaatat tattataatt
    61861 aatgttaaat cgagttaatg tattaagtgg tatgtaaatc attcagtttc caaagtgttg
    61921 ttcatcaagg aactgctcta agcttaaacg aaaaaaatat aattattgta ttttgaaatt
    61981 tttttctata taatgaaaac aggcaaaaaa ggtgcgtatc tatgctttat atgaaacacc
    62041 cagacaacga aattcagatt catataatag ctaccatgtt tctaaaaagg aacttgtaaa
    62101 aaagatagcg tttcaacttc ccagttgatt aaaattatca gcaggaaaac aagtatggtc
    62161 agcagtggta ttctaatggg tgttgtcgat atatccaagt tgggattgtc tgaggatgtt
    62221 atcaaataac ttgtgatagg tgcagtagat gtggagattc ctgatacgga tttattctca
    62281 ggggatggtt gcttcgggag atatggagcg taaccagttt attgatatgg taaccatttt
    62341 ttgtctaatt tgatgaaaga atcccaagaa taccatacag aagtaagaca ggaattaact
    62401 ggtggtataa actgataaag gcaaatttaa aaattttgat aggatgattt taagatttat
    62461 cttctatttt gtgttttaga aataattgaa atgttatgag gaggtatgta caaatggaga
    62521 ctgtaacaaa taatcaatca ggtgtagaaa ttcaactcag tctgtttgaa gctatcaaag
    62581 atttattgtt aattaatcct attttcttgt tgggaattat cttgattggt attttcttag
    62641 ttgtaatatt caagaaaaat agtgatattc caaaggtaaa aacatctatt ttatcactgg
    62701 caatgtatta ctacttatgt ttaacactat cgcatattgt tggtatccct acattcagtg
    62761 aatatataag actgtcacag ttaggggaaa cgttctttaa tccaaacata aatcttattc
    62821 cttttagtga tggatttagt ttgagtttta ttttgaatat tttacttttt gtcccattag
    62881 gttttttatg tccgcttatc agcaaaacct ttgaacgttt gagaaataca ttcttgattg
    62941 gttttggatt atctcttttt atcgaaacta ttcagctttt tacgttacac agagtaaccg
    63001 atatagacga cttactcacg aatgtaattg gaacaataat aggctatttc tgcttcaaac
    63061 tgattgctaa attgcgaatt gtaaaactac attcagatga tgactctatg gaacaggatt
    63121 acacagtatg tattccgatt gccattataa tagttgccct tatattagga ttttttagtt
    63181 aagttccaaa tagctaattt gggaattatg gaggtcatta agtgaataaa attactttta
    63241 tatgtttagg tgtaaaaagt atggagaagt caattaaatt ttatagagat ggattaggat
    63301 tttagattac aaacttttag tttaccaagg agatagcaaa gttgtgtgcc aaagggtaac
    63361 catttggaaa tccagacaac ataacagata gtacgttacc ttttacagga gcatactatc
    63421 tgttatattg tcaacatcct attttatggt ttgtgtgtag tttcctgtaa actgaactaa
    63481 ataattaaat agaagctatt agaattaata ataacagata gatattaata agcataaaca
    63541 aatagtaaaa tgtgtagata tagacgtttt taagtgaaac tattataaaa taattgactg
    63601 ataatcaact gacaaaacct atttttttag gtttaactga catttaactg acagtcagtt
    63661 tttactattt aaatagaagt tcgcttagat tgtcggaagc ttctttgtcc atttctttaa
    63721 gtacatgaga atatctattc atagttattt ttatatctgt atgacccaat ctttcagaaa
    63781 caactttcat attagttccc cctaaaagca gcagagtggc acttgtatat cttaaatcat
    63841 gtattctaat cttttttaaa ttattttttt tgtaaaattg tgaaacgttt ttcttagtga
    63901 agaaggtaga aacggattta aattggtgtt tagacaaact aaattatttc tgttttccag
    63961 gataccttac aattttaatt tgctttgtct tagtttttta attttcaatt tttcaattaa
    64021 ttcttttgga gctgataaag ctctttttga ttttggtgtt tttggctctt taaaagatat
    64081 tttgccatta gaatagacaa gaatttgatt aacatttatc agattgttat ctaagtcaac
    64141 atctgaccac ctaagcccag tagcttctcc cattcttaaa cctagaagta acattaaaag
    64201 aataggtatt tctatattat tactaagtaa cttttctatt agcaataata cttcttttct
    64261 attgtatatt tcatttgtag aatatttttc tttagaagga agttttataa aatcacatga
    64321 attttcttgt atttctctta atctatatgc ttcccttatt acagaactta gaaagctata
    64381 ccttacttta gcactagtaa aggtagaatt attaaagtta taatttacaa aagcttgtac
    64441 tgaactagga tttacttctg tgagttttag attcttaaaa agaggttcta tgtaattttt
    64501 aacccaagat tttgtatttg taacagtata tggagaccaa ttatttttat tcatgtccat
    64561 gtatttataa catctttcta caaatgtcat atcttttgga gcaacaaact tattattgtt
    64621 tatagtagat tttatttcaa ttaaatgttt ttcagcatcc tttttctttt cataacttcc
    64681 ataacttttc tgttttatct tgccagttgt ttcctctatg tactccacat atacatagaa
    64741 tttttcccct ctttttctta taaaagctga tttgatattc atatatgtac gctcccttta
    64801 taagttagtt ttataaaaga acagttgtac tactctaatt atgtcattat ctttttaatt
    64861 ctttacagtt acattataac acgtatttgt atacacttaa attaacagga agataaatat
    64921 cgttaaagga ggaaaagaaa atttaatgta gaattattaa gatatatagt atttcatata
    64981 taaaaggggg agatattgtg aaattaaaga aattattatc actagggtta gtgtcaatgt
    65041 ttgttgctgg ttttcctata tcagctaatg cacttgataa aattgataaa attcaaggag
    65101 cagataaata cgaaactgct ggtatcatag ctgacaaaca aagttatact actgcaatac
    65161 ttataaatgc agatagtaca atggctgatg ggctaagtgc gagtggactt gcaggagcaa
    65221 caaatgcacc aatccttttg actaaaaaga ataatatacc taatgcaact cttaaaagag
    65281 tagaaaaagc taaaaaggta tacataatag gaggagaaag ttccattgat aaagcaacag
    65341 aaacttttct aaaagataaa ggaatagaga ctagaaggct tcaaggtaat gataggatta
    65401 agacaagtta taatgtagca aaagaaataa attccattaa taaagtaaat aaagtaatat
    65461 taactaacgc tttcaaaggt gagccagatg ctatgagtgc tgcacctgta gcagttagag
    65521 ataaagcagc tatagtatta actgatggaa aaagtgttcc atttaataca actggtatag
    65581 aaagttatgc aataggtggc acttcatcaa tgagtgatag tttagtaaat gatactaatt
    65641 caactcgatt gggtggagta gatagatatg atactaataa aaaaatagta aataagttct
    65701 ataatggcgc aaaagaattt tatatagcta gtggtactga tttagtttat gctttagttg
    65761 cttcaccaat ggctaaaaat aatcctattg tactagttga tattggaagt aataaagata
    65821 tacttaaaaa tgctgcaaaa gtaaccggga taggaaatct aagtgataaa attatgctag
    65881 agtgtgaaaa taccatcaat aatattagtc ttccatcttc aaataaaaat ataatatgtg
    65941 gattaaagat tgattttgat tcaaatcaca taatttggca agataaagga agttatcttg
    66001 aatgttatga tgataacctt gaatattata ctgaaaaata taaagaccca acagttccta
    66061 taaatataga aaatacaagt acacaaaatt tagatgtaac aaaattaaaa gtttatggag
    66121 aagctaattc aacattatat atggggcaat acgctaaaat aaaaatggat tttaatagtt
    66181 tcggtgtatt aaagccagga gaaaaatgta ctatttattt agatataatt tcattagatc
    66241 catcaagaag catgacatta acattttctt atgtaggtga taaagaaaat tataaatatg
    66301 aacaaggatt ggcaagtaga atattatttt ataacccgga tggagaagat ttttttgaat
    66361 ataaataact agtgggtatg taaatgtctc gatattgctc atatgttgag ttattatatt
    66421 tagtttcata tgaactatat ggattttaaa attaaaaata cttaaaaaca tttactttaa
    66481 tagtaggtgt ttttttgatt aaaagaatat tattataatg taaaaagtaa taaataggta
    66541 aaatatgtaa gaattatatg ttataatatt tttagcaaga agatgtaatc tacaatttat
    66601 agagtggagt tcatgtatca aaaaattatc ctcccgacgt taagaaggga ggtgaatata
    66661 catggataat tttttacaag gcatactagc aagtctatct gctagcttaa tagtttatat
    66721 agctagcaaa ctatttagaa agcgtaaaaa accactcaaa gcggcaacta agagtggttg
    66781 ggaatttgat ttaaaaatca gattccataa aactaagtaa tttctaaatt atgaactcca
    66841 ctctacagca aaatagattg tagttcttct tgcttttatt atatcacaaa ttggtacaga
    66901 tattcaaaaa taatattttt atgatataat aaagtcatag aaattttgca gtgttcgatt
    66961 ttttgaagaa attaaggctt aacagttgaa atataaggca ttgaggactt gtgataagtg
    67021 ttatcaattg cactactcat ggttcactgc aaatttgaga gatttgtata tgtgtaggca
    67081 ttggaaatac tcaatttatt ttggggtttt atattaacta tatggaatgt aaatcattcc
    67141 taacgttagg cattgtgtgc tgcacctctt gtgttttata ttaactatat ggaatgtaaa
    67201 tttcatattc catgcattct gcttacactt aatagaaaag ttttatatta actatatgga
    67261 atgtaaatta tttctagcga cttggtcggt tgctccattt agcttgtttt atattaacta
    67321 tatggaatgt aaattttttc aaatctgata tatcgacctt aaatttcgtg ggttttatat
    67381 taactatatg gaatgtaaat gatataaact ttgggagttc agaagaagga ttaaaaagtt
    67441 ttatattaac tatatggaat gtaaattaga gatgaactca gatagaaaaa catgtagatg
    67501 tgtgttttat attaactata tggaatgtaa attcttcaac agttgtgtta ccatctccta
    67561 cacgcacagg ttttatatta actatatgga atgtaaattg ctacgtaacc agtttcaccg
    67621 ctttcagtat ataacgtttt atattaacta tatggaatgt aaattgttgg tatagtttca
    67681 gaaaagaaat tcgaaacact tgttttatat taactatatg gaatgtaaat tgtatccaaa
    67741 aaattacaat aatcatacga actgctaata taagcagtca atatatgtgt acatttatct
    67801 attttatctc taaatatata aattattatc cccctaaaaa actttcataa aaacaaaaag
    67861 aacaatatct ctttggcaac tggctgacat aactcataag atatttatat aagtcttagt
    67921 ctctatagct ttgcgtcact agatttctct agttttgccg atttagtttt attctataac
    67981 taaataatac aatagaatta gttattattc aataagattg tttgaaaatt gaataaattt
    68041 ataggtatga acgtaatttc acacttaaaa aacagactaa atacgtagaa tatatagaaa
    68101 aatttacctg tagtttttgt gataaaaagc tcctattatt aatttaagct agataagttt
    68161 gaggaagtga cataaattga aagaaggaat atattatgtt gaataaaaaa ttaccagatg
    68221 cagaattaaa aattatgaaa tacatataga atactggtta taaaactgta atatcaaaag
    68281 atgttgcaga tgaaatagaa aaaacatatg cttggaagca tacaactaca attacacttt
    68341 tagctagatt aactaaaaaa ggttttttca tatcgcagag aattgggaaa catgtacact
    68401 atagagtatt aataaaagag aaagaatatc tcaaaataga gggaaaaaga atttttggag
    68461 gtctacataa caatccatta tcagaattaa tttcaaaatt gcatgatgga gaagaaataa
    68521 ctgaagaaaa aataatagaa ataggaaatt ggataaaaag ctggaaagat gaagattaat
    68581 aaataaaagg tagctactaa tataagtgct accttttttc ttgtggataa tattggggat
    68641 aagttgtgga aaacttgtgg ataaaatatt ttgctgacat ttaactgaca taagtattag
    68701 attttgattt aaaaatgttg aaatatcaat aaataattaa atagaagtta ttagaattaa
    68761 caatagattc ctttttcaaa gaaatctttg atttcatcaa ggttatcagt ttttccaagt
    68821 gctaagataa gttttattct agctttttga cctggtaagt ccccaccaaa tatacaacca
    68881 agatttttta aatctctacc agaaccaaag taaccataac tatcgaatac tctacctgaa
    68941 tgacatctag aaactataac tacaggaagt cctttttctc tagcatattc aactccagaa
    69001 agcatttgag gaggaatatt acctctaccc attgcctcaa taactatacc tttataacca
    69061 ctatcaacag caaatcttat aaagtcagca ttttctccta catatgcttt aaataaggca
    69121 actttagatt ctaccttatc agtatctata attgttctat ttacaatatc tcttgtaagg
    69181 acaagttcgt tgcagtcaat tattcctaat gggccactag ttagagattt aaatgtattt
    69241 agagaaagag tatttgtttt agtagcttca gaagctaata atacttcatt gtttaaaact
    69301 acaaggactc cttttcccat agcatctttg gatactgcag tacaaactga tgcagataaa
    69361 ttacttgaac catcatatcc aagttcagaa ctacttctca tagcacctgt gactattaca
    69421 ggtttgatgt tgtcaattgt aaggtctaga aaataagctg tttcctctaa actatctgta
    69481 ccatgtgtta ttacaacacc tgtaatatct tttcttgcta ataaatcatt tacataattt
    69541 ttcaattcca tcattctata aggtgttatg tgaggtcctg gaatttcatc aaaattaaaa
    69601 acttcaacat ctgcaacttt atcaatgttg gtaaccattg atagtatttg ttcacctgat
    69661 aaagttggta tagctgctcc gactttatca tctactgtca tagatatagt tccaccagta
    69721 aaaactatgg caactttttt cttaatactc atactattct ctgcctttca taaataaaat
    69781 ttataattcc gataggatta tatcaaaaaa agtagttaag gtctacataa actcttttat
    69841 tcatataatt tagatatttc agtgtaatat aattatttct taattttttg ccattcaacg
    69901 atataaccat ttaatatctc tcttatggct tttcctatct tagtataagc ttcatcatat
    69961 agatttgcta gagatattct aattgaccat tcaggaccat agaatccatt tccactaagt
    70021 aaaactactg aatagtcatt agctaattta taaagaacat ctactggaga tgcagtttta
    70081 cttataaatt taaataagtc ttctccgtag tttaatttag cccattcttc aatatcaaat
    70141 tgagtgtaat atgcagcatt gtttttgttt tcattaattg gaagctctaa agcttcaaat
    70201 aatagttttt gacgtctatg gcagatactc atattgagtt ttttgtaggc atctactttg
    70261 tcaatcaatg caaaagcaga gaaaaacgcc atttgtactt gctgtggagt agaaagacca
    70321 gcagtatggt tgagagcaac aagtctgcta tcagcaacaa ctctttccat aaatgaaagg
    70381 ctagaagggt taagactcat atcactgtaa cgcttatcta ctgatttttt taattctcca
    70441 gttaaatcat ttattttctt atcaaaaaca tttttcttat gaagggcaaa tgtaccaagt
    70501 ctccatcctg taactccaaa atattttgaa taagaatata ctccaacagt gttgtaaggt
    70561 agttttgaca ttaaggatga aaaatcttct acaaaagttc catatacatc atctgatatt
    70621 atcattaaat ctttattata attttcaatt acatctatta gattattaca agatgtttca
    70681 tccatagcta tagaagcagg attattagga ttaacaacga ataaagcttt aatagatttg
    70741 tcacgaagtt tctctaattc tttttttgta tattgccatg tatgagcacc tttttcatca
    70801 acttcattag catttatatt taccacttta aagtcgtaac gaggaaggtt aggtatttca
    70861 agataaggtg taaatattgg agtcattaag gctatggtat ctcctttttt tagaagttca
    70921 tttgccatta atgaatcaaa tatatagcac atagcagctg tagcaccttc aacagcaaaa
    70981 atttctacat caccatattt acctgatata tcatatttca tttcttgaac taggtaatcg
    71041 tgtactattt tttccatatg aggtaacatt ctatctggaa gtggatagtt atcaccaata
    71101 attccatcag ctagttcatg aacaaattca tctccatcaa atccaagttc atttatacca
    71161 aagtttataa tatctctagc taaatatgct ccaggcatat ctatattttc atttataaat
    71221 ttaaaaaatc tttctttaat accctttttt tgaggcattc cagccaaatg acctaaatcc
    71281 caattagaac gagtttcagt aatagcgaaa tgaccaaaag taaaaaaggc ttctctagca
    71341 gttgaacaag tccagtttgg atttccacga cctgcatcaa gtaattctct gttattttct
    71401 tttgcagtgt attttgcaat atctattaat ttgtccttaa attcaaaagg gcttattttt
    71461 ccatatatgt ttttgatttc tttataagta tgatttttca tgttaaattc ctccttatgt
    71521 aataagtttc acatttttat aataaaataa acaagttgca aaaatgatta tactttaatt
    71581 ataataggtt tatagaaaaa gtaaagtatg aaattaaaat aataactcta tttagcctta
    71641 ttaaatatat gttaaaataa aaaatacctt ttaataaaaa atgattgtta tataaaataa
    71701 atattcaatt taattatcac cttagttttt ttattgtatt ttattatata tgtatagttt
    71761 tctaattttg aataaaaata aagtaaaact ttcttttata ttgaagttgt ttagtaagac
    71821 atgtatttta ttaatttatt aatcttaata atgttaaata aaatcaaaaa aatagatttt
    71881 gggtaaataa aataatataa aatataaatt ttaataaaaa taataaagta tatattattg
    71941 ctttaataag aatgtaaaaa atatttttaa attatcattt aatgtaattt aaatgaattg
    72001 aagatatatg tttattgtat gcaattgttg tatttttagg ttttttgtga ttaattaaaa
    72061 aattataaaa atcgtttaca tgttgatttt taaaaaaaaa agatatagaa ttatatagaa
    72121 agtaataaac aaaaattgga tttacgggag tggaatgaaa tgatgaatgt taccttaaca
    72181 gatgtgaaag aggctagaga gaccattaag aacatagtaa aaagaactga cttacttgaa
    72241 agtgttaaac ttagcgaaaa aacaggagca aatgtgttct ataaatgtga gaatcttcaa
    72301 aaaacaggtt cttttaaatt aagaggagct tgtaacaaaa tagcgagttt aacagatgaa
    72361 gaaaaggcaa atggagttat agcttcaagt gcagggaatc atgctcaagg agttgcattg
    72421 ggagcaaaaa tgactggtat aaaatctaca atagtaatgc cagcaactgc gcctttagca
    72481 aaagttagtg caactaaagg ttatggagca gaagtagttt taaatggtgc ggtttatgat
    72541 gatgcatatg caaaagctgt tgaaatacaa aaagaaacaa gagcaacttt cttacatcca
    72601 ttcaatgata agtatgttat agcaggacaa ggaactatat ctcttgaaat tttcgaacaa
    72661 ttagacaata aagttgatac tatactatgc ccagttggtg gaggaggaat catctctggt
    72721 gttgctgttg cagcaaaagc attaaatcct aatgtaaaaa tagtcggtgt tcaaactgca
    72781 aatatacctt caatgaaaga gtcaataaaa aatggaaaag taacaacagc atttaatgat
    72841 actacaatag ctgatggtat agctgttaaa actccaggag atttaacttt tgaaataata
    72901 aatgaattgg ttgatgaaat agtagtggtt gaagagacag aaattgcaga atcaatatta
    72961 tttatgatgg aaagtcaaaa aatagtttca gaaggtgctg gtgctgtatg tacagctgct
    73021 atattaagtg gtaagtatgt accagcaaaa gatgaaaatg tagtttgtat aatatctggt
    73081 ggtaatatag acataaatac gttatataga ataataggtg ttgctttagc taaagaagga
    73141 agaagatatt catttagtac aattatggaa gataagccag gaaactttgc tgagttaact
    73201 agaataataa gtgagaatgg tggaaacatt ttaagtgcta atcaaggtaa attatcagca
    73261 ggggaagctc taggaaaaca aagtgcggaa tttatattag agacaataga ttatgaccat
    73321 atagctagaa taaagaaagc tatagaagaa aaaggtttta aaattataga attataataa
    73381 aaagtgtata taatatacaa gaaaaatagt ataatagtgt atgaagatac attttatgtg
    73441 tattagatta acgaattagg aggattttaa aatgaaacat gaagtaatac atactaatga
    73501 tgcaccagca gctttaggac catattctca agctataaaa gctggaaact tgttatttgt
    73561 atctggacaa gttcctcttg taccagagac tatggaagta gttgaaggag atgtacaagc
    73621 tcaaactgct caatctttaa aaaatcttaa agctatatta gctgaaagtg gagctgattt
    73681 ttcaaatgta gttaagacta cagtatttat aaaagatatg aatgaatttg gggctataaa
    73741 cgaagtttat gctgaatatt ttggagaaaa taaaccagca agagcttgtg tagaagttgc
    73801 tagattacca aaagacgtta aagttgaaat agaagtaata gctgtattat aaaaatacaa
    73861 tatttttaat ttgaaaaggc tgaaaaacta tattctagtg attcagcctt tttataaaaa
    73921 aataaatcac aataaagaac atgataaata ttgtcgaatt aaaaaataaa attaaaataa
    73981 atattgcaat atttttgaga aggttttata atgagtatat aatttgaaac gtgtaactat
    74041 taaattaggc atgagttctt ggatattttt atcctgagga ttcatgcctt ttttgtattg
    74101 aaattcaaaa atctatttaa taaggaggaa gtactgtgtt aaattttttc aagaaaaata
    74161 aaagctataa gttacatgca gtagtgagtg gtaacagcat taatattgag aaagtaaacg
    74221 attctgtgtt ttcaaaaaag ttaatgggag atggagttgc aattattcct aatagtaatg
    74281 ttgttgtagc cccttgtaat ggtaaagtaa ctgtgcttac agaatcaaag catgcctttg
    74341 gtatggtctc agatgagggt gttgaaatat tagtccacat aggaattgat acagttagtt
    74401 tacagggtga ggggtttaaa aatgaagtgt ctcaaggaga tactgttaaa aaaggtagtc
    74461 caataataag ttttgaaaga gaaaaaataa attctcaggg aattgattgt acaacaataa
    74521 taattgtatt aaatcatagc gaattttcag aaataaattg catggtagaa aatgaagttg
    74581 ttgcagggca agatactgtt attgaaatta tgaaataata ggaagggatt aattcaatat
    74641 gagagtagta aaaatcttta ataataatgc attatctaca gtaactaatg ataggaaaga
    74701 agctatttta ctaggtcttg gtataggttt taataaacgt ccaggagata aagttaatga
    74761 agataaaata gaaaagattt attatgttca agaccacatg caaacaaaat ttttagaatt
    74821 attaaaaaat gttagtccag aagtgatgga tgcttcacaa caaattataa gtcttatacc
    74881 aggaaatgag ggaaaattta ataataaagg gatattatct ttagtggaac atattagttt
    74941 tgcaatagat agaatgaaaa ataatgtttt tttacctaat ttaatgcttt tggatataaa
    75001 gatgatgtat tcaaaagagt ttgaattagg agtaaaagct ttagaaatta tatatagagt
    75061 atgtcatatc catttgcctg aagatgaagc aggttacata gctttacatt ttgtcaattt
    75121 acaatcaaat gataatttgg catatgatac tttaaaattt gtaaaaggta gtattgattt
    75181 gattaaagaa tgttatggat tagaattaga cgaatcgagt ttaagtacat tacgttttag
    75241 aactcatctt aaatttcttg ctcaaagaat ttttaagaat gaaatttgtc aggatgataa
    75301 aatgattgaa atgtatgatt atttaattaa caatcatcct aaaaataaag aatatttaga
    75361 aaagttaaat gtatatattg aaaaggagtt taggtataaa ttagataaac ctgaaaaaat
    75421 ttatctacta ctacacctaa caaaaatctt ataagaatga gatgaattag taatagaaat
    75481 atataatttt agggggggat aagattgaaa gaaaaactac ttggaggact ggaaacattt
    75541 gcaaaggcaa tggttcagcc attaatgtat ttatcgagtg caggaatatt aatggtggta
    75601 ggtgtactac ttacaaatga tatgttatta ggagtacttc cattttttaa atgggagcca
    75661 attcaaatat ttggaagcct aatttataac tgtataatgg ctattattaa taatcttgct
    75721 gttatatttg ctataggtat tcctgctgca ctagcaaaaa aagagaagca caaagcagcg
    75781 ttaataggat ttatgagtta tataatatac cttacagctt ccaacactat gttaacatca
    75841 ctaggtcaac tagcaaaaga aagcaagatg cttggactga tagggactgg acaagctact
    75901 attttaggta ttcaagttgt agatacaagt gtatttggtg gaattatttt agggtgtata
    75961 gctggatatg tatttaatcg tacaagcaac aaaacattta aaggagcttt acagatatac
    76021 tcgggagcaa attttagttt tatatgcatg ttctttgtat ccattttaat tggagttgtg
    76081 agtaacttta tatggccatt agctcaacaa ggaatagctt ctcttgcaaa tgtcatcaaa
    76141 gatacaggta catttggttt attcttatat ggttttttag agagaatatt agtaccaact
    76201 ggacttcatc atttaatcta tacaccattt cagttttctg atttaggagg agtgcttaaa
    76261 gttggtgcag atacagtttc aggagcctat gctatagtta tgacagaaat gaatttacca
    76321 gttgagcgtt tctcagatag tatatactac atggcaactg gatttacaaa gatgtttggt
    76381 tatataggta taggagctgc ctttatttat actgcatata agcaaaataa gaagaaagtt
    76441 aaggcaacga ttattccact aataattacg gcagtattag cctctgtaac agaaccatta
    76501 gactttatgt ttgtattctg tgcaccattt ttattctttg tacatgcagt tatagctggt
    76561 ctttttatag ctttattaaa aatatttgat gttactgcat tatgtggagg gaacttatta
    76621 gtatcaatta taatgaatac tacagtaggc gtagaaaaaa cacactggcc agtaatgatg
    76681 atattaggac ttgcacaaat tattgtatat tttgtagtat ttagtttttt aattaagaaa
    76741 tttaattata aaactccagg tcgtgaagat gagagtacag ctacaggcga agaacaatca
    76801 aaacaactta atttagatgc agggatagaa aatataattg atgggttagg cggaaaagaa
    76861 aatattaata cagtagaaaa ttgcatcact agattacgtg ttaatgtaaa agatgaaagt
    76921 aagattaatg aagatataat taatttaacg ccaaatagcg ggattgtaag gaaaggaaaa
    76981 gatatacaaa taatttttgg acttcatgtg catgaggtaa gacgtgcagt agaagatttt
    77041 ttagaagctt attaaagata tatatttata tagcaagggt aaataacagc tttaagtgct
    77101 gtttactatt tattaaagat acatataatt tatagaggag gaataattat tatgattatt
    77161 actattgttg gtggaggaag cacatttact ccaggtattg ttaagtcaat tgcattaaga
    77221 gaaaaagagt tagagcttga ggaaattcgc ttatttgaca ttgataaaga acgccaagat
    77281 aaagtagctg tagttgtcaa ctggatacta aaagaagatg ttaaaacaga tgttaaacta
    77341 actgtaacta attgtgaaaa agaagcgtat acagatgcta catttatatt tgcacaaatg
    77401 cgtgttggta aatatgaaat gcgtgagcag gatgaaaaaa ttccattgaa atatggttgt
    77461 gtaggtcaag aaacttgtgg ttgtggaggg atggcatacg gactgcgtac aatacaacca
    77521 atgataaagg ttattgatga tgtagagaaa tatgctaaaa aagattattg gatattaaat
    77581 tactctaatc ctgctgcaat cgtttctgaa gcttgtcgta agttaagacc aaatgcaagg
    77641 attataaata tctgtgatat gccaatagct attatagata tgatagcggg gtcattaaat
    77701 atcaatgacg ttcataatat acgctatgat tattttggtc taaatcattt tggctggttt
    77761 acttcaatag attataaaca tagagactta atggaagaga ttaaggaata cataaaagaa
    77821 aataaaatat tattaccaaa atcttatata gaaaacttaa ataaaagtcg tagtacagga
    77881 aatcgccatg ctgtaagtag ctggatacat gtttgggaat ctgtttatac aatgttagaa
    77941 agcttcccaa aatatctgcc aaacacttat ttaacatact atcttttaag taaagaaaca
    78001 gtggaacatt caaacataaa tcgtactcgt gcaaatgagg ttatggaaag ccgtgaaaaa
    78061 gacttatttg aaggtgtgag acattactta gaaactggag aaatttctga aaaagcattt
    78121 tatgcaggtt cacatggaga ttggattagt gatttggcag tttcaattaa aaatgataca
    78181 cgtagtcgtt ttttagttat aactgaaaat cgtggagcta taccaaacat gccatatgat
    78241 gcaatggtag aaattccagc atatattggt aaagatggtc cagaagttat tgctcgaggt
    78301 ccaatacctc tatttcaaca aggattaatg atgcaacaac ttaacagtga aaaattgata
    78361 gtagaagctt atatagaaaa tagttatgaa aaagctttac aagcattcac attaaataaa
    78421 actattccaa gtatgaatgt tgctaaagaa attttggatg aaatgataga agttaataag
    78481 gaatactggc cagaattaag atagtaggat aaaggaaagg gtttttattt aataattaga
    78541 tatgttttca gagtctttta tcaaaatgta ttcacaaaca tatggcaata tattaaaata
    78601 aagagtgata tttttagaag aatggggtga tgcaatgaac attataaata aaattttttg
    78661 ggaagaagat aggattggca tgattacaaa taatttagag gcagatacac atagccactg
    78721 tatgttacaa ctgttcttag ggatagaaga cagcattgaa ataacagtca atgaaaaatt
    78781 agttaaatgt aattgtatca tagtagataa aaatatctct cattcatttt cagcaagaaa
    78841 aaaagtttat tactctgcta ttattgaacc tacatctata tatgcagaac agcttacctc
    78901 aaaaatgaat gattttggct actggatatg tgacaatgat ggattagaga aattacgtca
    78961 acaaggaact tttttgatag ataactctag taaagaacaa tatttaagat ttatggaaat
    79021 gcttaataac tatttgaata tacctataac tctcaaacat tatgatgaca gaattactga
    79081 attgctcaat ttattacgta cttgtaattg tgataatcat actatttcaa gttttgcaga
    79141 taaagtgtcc ctatcagcaa gtagattatc acatctgttt aaagaacaaa tagggatacc
    79201 tttaaaaagc tatatcttat ttcatcaaat ggagtgtgct tttagagaat tactttctgg
    79261 gaaaaatgtt accgaagctt ctatgactgc tggattcgat actccatcac actttgcagg
    79321 gacagttaag cgaatgatgg gaatgcctgt ttctttatct ttgaaagata gcgaattttt
    79381 gaaagtatat taaatattct cgttatataa tgaagttgta ctaagaaagg ggctttgtta
    79441 tgaataaaga atatttagag gaaacaaaaa tgttaaatta taatgaacca caacttaagc
    79501 ttttggtatc atcaaagaat tggttggaat tagatgattt tcataaaata aaatctattt
    79561 atgagtttgt acagaatgat attttatttg gatataatgc ttctgatatg ttaagcgcaa
    79621 cacaggtatt aaatgatggt atgggacaat gcaatacaaa agctactctt ttaatggcat
    79681 tattacgtgc agtgaatatc ccttgtaggc ttcatgcttt tgatgtaaca aaagattttc
    79741 aacgaggagc tacatccaaa ttgatttcac tattagcacc aaaatatatt cttcatactt
    79801 gggtagaggt attttatcaa gataggtgga ttgctcttga gggtgtcata actgataaaa
    79861 aatatttaga agcaattcag aagaaatttt ttaatcatgg tggaacattt aaaaagtatg
    79921 caatagctac aaatgatttg aaaaatacat ctattgattg ggatggtaaa gatactttta
    79981 tccaaaaaga agctatcgtg tatgattatg gtatttttcc atcaccagac gtattttttt
    80041 ctactcattc acaacatatg tcaaaattaa aaaatttcat atatgtacat cttatcagaa
    80101 aaataatgac aaaaaatgta tgtaaggcta gaaacaatta tattgataaa aacgaatgag
    80161 tgtttaagag gtaactatat aggaaatata tagaataatt aaaactgtac tggttaaaat
    80221 gaataaaaag gagttatcac agagcaaact taaattgttc ataggataac tccttttctg
    80281 tattaacttc taataatttg atgtcttttt ataaaacata tagatacaat taaaccagta
    80341 aatgcaagag tagctgctat tgaaaatgca acatctattc catgaatgtt agacagcatg
    80401 tttgaactca tatgagcttt cttagtagca cttgtcatta tagtaataag tatagcagaa
    80461 cctatagctc cagatatctg acggagtgtg ttattaattg ctgtggcatg agatataagt
    80521 tctctatcta atgttttaat tccccatgta gttaatggca ttaaaaccat tgaaatacca
    80581 atcatacgaa tacaatacat caatgacaca tgaattaaag atgtatctct gcctaaaaaa
    80641 gcgaaggata aagttcctaa aaacaaacat cctgttccta atattgatag agcatgaggt
    80701 ccatatttat ctaaatgacg tcctgcaatt ggattgagta caaccatcaa tatagcacca
    80761 ggtaacatta atgaacctga agataatgca gaaaatcctc ttacagattg aacgtacaga
    80821 ggaatcatta atgtagcaga catcattgag gcatatacaa tcataattaa tattgtagaa
    80881 ataagaaatg gtttattttt aaaaaccctc aattctaaaa aaggttcttt agattttaat
    80941 tgacgcaatg taaataaaat taatgacata attcctatta atattggtaa atatgtggca
    81001 atattagtcc acccataatt accttgatta gtaaatccaa ttaataaacc accaaaacct
    81061 agagatgata gtactattga aggtatatct aattttagtt tttgagtttc cccaacattt
    81121 cttaatagaa taaatgatag tataacatct aatatagcaa taggggaaag aaacagaaat
    81181 aaactatgcc aaccaaatga atctactaac catcctgata aagttggacc tattgctgga
    81241 gcaaatccta ctgtaatacc aactaaactc atagctgcac cacgtttttc ctcagggcat
    81301 aaatagagta taataacttg taaaagtggc attagagaac cagaacccat tgcctgcaac
    81361 acacgagcaa tcaacatagt tgaaaaatta ttagaaaaaa gtgcaataat acatcctata
    81421 gaaaagatag acattgatgt aataaataac tttctagtac taaatcgatt tattaaataa
    81481 gctgttgtgg gtacaataat acctgcaata agtagataaa ttgttgttaa ccactgacca
    81541 atatcagcag taattttaaa atctgacatt atattaggta atgctgttga caatagtgtt
    81601 tgactgagag aacttataaa agcaccagtc attacaattg ctattattaa atttcctttt
    81661 ttattgttac tttgggtttc cataatttat aacgtctcct tttttaattg atttttaatg
    81721 ctgttcaata aaagtacttt tggtcttaaa gtgtcttctt tttctaaata tgcaatcatt
    81781 gcatccataa ttaaagaatc atattgttga ttttttcctt cgacgtataa tttttttatt
    81841 gcttctagta atttaggatt ttcaaggaga gaagtatcag ttttgattag ttcttctata
    81901 tcttgtatcg ttttaaaaaa tagcgattta ttatgaaggg aaaagtaaac ttcatcttct
    81961 tgtttatgta aaaacattga tttttcaatt aaaggattta gattatccat attttctaca
    82021 actaaatcta gaatttgtat agtaaatttg gcaataaagt atacatttat ttctcttttt
    82081 tgtacatgaa taatgtatat aaactgatga attagagcat ccaataagaa aactaaatca
    82141 aatataattt tttctatttt atcaccatag ttatcaaata agcttagttt gtattcattc
    82201 aataagttag ctcttgattt tgtttgcaat ttacttgtag ccttacgttg ctcttcagaa
    82261 aagttttctc ttatataggt atttatggta gttctagaaa aaatattttt ccatacagca
    82321 attatctttc tgtttaactt ttctgatgat ggaatgtcat tattttcatt tatggaatgt
    82381 ataatttcta aaaacttttc atcataataa ttgattattt cgcagataaa actttcctta
    82441 gtttcaaaat gcttgtaaaa agtagcttta gacattttac actcttttac aacatccgat
    82501 attgaagtat tttgaaaacc tttttggtca aaaatttttt gtgcaatagt tataatgagc
    82561 ctttgttttt ctgtcacttt attatctcct ttcacgcaaa aacgtttagt actatatagt
    82621 acctacaagt tttgataaat atattataca tcatttggaa aaaaaatcaa tatatagact
    82681 gtaatatgaa atttttgaaa aatttgttgt tatttttagt cgtataaaaa agaaacttaa
    82741 gagtttctta agaattgttt aaagaagttg caataaatta gtggtatgat tatagtatga
    82801 aagagaatgt cttgaaattt ttctgtatca aattccaatt atggaggtgt agtatatgaa
    82861 aaagaaaatt tgtgtagcaa taatttttgc tctatttata gtttttgtag aaatgtttat
    82921 tgatagctct agcgaaggaa gtaaaattat gacagctgaa ttaagtaaat acaggaaagg
    82981 cgtaaatgta ttatcaataa acaatattat agtagcgaat aaaaaatata gtttgcccaa
    83041 agattattct cctcaggaaa gcagtgaagc gagagatgct ttttataaaa tgaataaaga
    83101 tgctcaaaag tcaggattaa atttaaaagc ttttagtaca tatagaagtt atgaatatca
    83161 agatagatta tttaagtcat atgtaaaaga acatggagaa aaagaagcaa atagattttc
    83221 agctaaacct ggagagagtg agcatcaaac tggacttgct tttgatattg gaggagatga
    83281 ccagtcttgt tgggcaaata aaaagttcaa caatacaaaa gaagctaagt ggttgtatga
    83341 aaatgcatat aaatatggtt ttatacttag atatccagaa ggtaaagaga atataacagg
    83401 atatatgtat gaatcttggc actatagata tgttggaact gaacatagca aaaactttgc
    83461 aatgaataat ttgactttag aagaatattt acatataaat taagagatgg tttgttaaaa
    83521 ttaggttaaa ataaaaaata acctagacta ttagctgtgt aaagtgatat aatgtaaata
    83581 caatttaaga taactactcg aaggggagta gcttacacga aagtggtaat taatcgtcaa
    83641 gacagtagaa atactcggtt aattagcaag ggattccttg atagcaagac cttcaaaata
    83701 agcattaggt atgtttgttt tggggtcttt ttttattcaa ggaattttta tttaaaaaaa
    83761 tttaaatttc aagtatacat aagagccccc aaacataaaa taaatttatt gaagaggagg
    83821 gcaacaaatg tcttacaaaa gtacttatca agaagtaatt aaaaatttag actcaagttt
    83881 atctggtttg agtaaagaaa gagcagaaga gttgttagaa aaaaatggag ctaatgaatt
    83941 aaaagaagca gataaggttc ctacttataa gcttttccta gaaagtttta aagacccatt
    84001 agtaataatt ctattgatag ctgctttagt acaaatattt ttaggtgaaa cagtggagtc
    84061 tataataatt tttgcagtta ttattataaa ctctgttctg ggtgttgtac aaacaaaaaa
    84121 agcagaaagt tcattagaaa gtttgaaaaa tttatctgca ccaaacgcaa aggtaataag
    84181 agacaataaa aaaatgacaa ttccagcaaa aaatctagta gttggagata ttgtattctt
    84241 agaagctggc gattacatac cagcagatgg aaggcttata gaagcacaat ctctcaaggt
    84301 tgtagaaggg atgttaactg gagaatcaga accagtttta aagcatatag aaaaaataag
    84361 tgaagatgta agtttaggtg accagaaaaa tatggtattt agtggtgctg tagttgttta
    84421 tggtagaggt tcttttgtag tgactggaac aggtatgaat actgagatgg gtaaaatagc
    84481 tgggctttta gaaagtgctg aaaataaact tacaccattg caacaaaaaa tagaggattt
    84541 tagtaaaaaa cttggtgtag gtattgtaat acttgctctt ttaatattta taattcaagt
    84601 agcaagaaat tactttatgg ctggaaatgg agaaatgaca caaattattt tagattcatt
    84661 catgttctct gtagcagtag cagtagcagc tataccagaa gctctatcat caatagtcac
    84721 tatagtattg gctgttggaa caaatagtat ggcaaaaaaa caagccataa taagaaaatt
    84781 accagcagta gaaactttag gttcaacaag tgtaatatgt actgataaaa caggtactct
    84841 tacacaaaat aaaatgacag tagttgatta ttatatgtat ggaactaata atgatgagtt
    84901 agataaagaa aaagtaaact attcttatca tgctaaatta ttgactatgg tttctacatt
    84961 gtgtaatgac tctcatataa ctagtgatgg taaagaagtt ggtgacccaa cagaagtggc
    85021 tttaataaat tattcaagta aaaatgattt agattatgat aaattaagaa gtaagtatat
    85081 cagaataaat gaaataccat ttgactcaga cagaaagctt atgtctactg taaatagtat
    85141 atatggagat tatataatgt ttacaaaagg tgctccagat attgtgttta gtagatgtaa
    85201 atatgctttg aaaaatggtt caaaagtaga tataacagat gagataatag aagaatataa
    85261 acataaaaat gaagagttct caaatagagc acttagagta ttagcttttg caataaaaga
    85321 tgtcccagaa gaagatttta taccttcaat tgatgacgaa catgacatga cattggttgg
    85381 aataatggca atgatagacc caccaagaga agaagtaatg gatgcagtaa aagaggcaaa
    85441 gagtgctggt ataaaaactg ttatgataac tggtgaccat aaaacaacag cagcagcaat
    85501 tgcaaaagag attggtataa tggaagaagg agacttggct cttactggaa aagagcttga
    85561 tgctttgagt gaagaacaac tatatgaaaa actagaaaat ataagtgtat atgccagagt
    85621 ctcgccagaa aataaaattc gtatagtaaa agcatggcaa cataagaata atataacagc
    85681 aatgactggt gatggtgtta atgatgcacc tgcattaaaa caagcagata taggaatcgg
    85741 tatgggaagt ggtactgatg tagcaaaaga tgcttctgct atggtattgg ttgatgataa
    85801 ctttgcaagt atagtaaatg ctgttgaagt tggtagaaca gtatacagta atataataaa
    85861 atcaattact tatctatttg caggaaactt aggagctata gtatccatag tatttgcagt
    85921 gtttgcaggt tggtcaaatc catttacagc tttacaactt ttgtttataa acttagtaaa
    85981 tgactcttta ccagcaattg cattagggtt tgaaaagtca gaaaaaaata taatgaataa
    86041 acctcctaga cacccggatg aaagtatact agcaggagga acattgaagg cagttgctat
    86101 aagaggttgt ataataggtg tagttacaat aattgcacaa tatataggat tacaagtatc
    86161 tccagaactt ggagtagcaa tggcattttc aacattgata ttagctagaa taatgcaaac
    86221 attacctgca cgttctaata atcaaacagt aataaattta ggttttttca gcaataaata
    86281 cgttatagga gcagttttag tctgtcttgg gctttactct ttaacactaa taccagtgct
    86341 aagaggaata ttctctatac caacaacgtt tggattgtac cagttgggga tttgcctagg
    86401 gttagcatta atttcatcaa tattaatgga acttaacaaa ttaataaata gaagtgaagt
    86461 aaaataatca aaaaattaaa gggcatattg attttaacaa tatgcctttt ataaatagtc
    86521 taatttattc tatattatcg attatttgga atgcttttat aacttcaggt aattcttttt
    86581 cttcaggcat tctaaatctt atgaaatttt tatcaagtcc agtaaaatca cttccagata
    86641 taactcttat ttttaattta gcaaactctt gttgtaaatc tatgtccttg tttttatgtt
    86701 caactgtcat aattgataca gtatcactag tttcagctat attaaggttt ttccatggaa
    86761 ttaataattg attttttata tcttttgttt tttcttttaa ttcttctata aattgaacat
    86821 catctaataa atttgctgca atactacgag atatttcaga cacttcgtaa ggagtagaaa
    86881 tctttttaat agggctaaca agttgttctg gaagaactgc atatcctgct cttagtcctg
    86941 ctaaaccaaa tccttttgaa aatgttttta aagcaattac attatcataa ttatttagaa
    87001 gcttgacagc tgaattttct tttggcatgt attcaccata agcttcatct accataactg
    87061 ctatatcata tttagctgct tcctttacta tattttcaat tgatgataaa ggtataattt
    87121 gtccagtagg attgtttgga ttgtctatgt aaattacttt atattctgga tttatttttt
    87181 caataaattc tttttcatta aacttgaaat tatcctcttt ctttaaaaga acataatcat
    87241 aagttgcacc atgcattttt atatctgtct catattctga gaactgaggt gaatatccta
    87301 aaactttatc tcccttttca ataaatagtc tatttaaaag gtaaataaca tgtatagaac
    87361 catctgccaa acaaattctg tcagtatcta atgctataaa atctttccaa taatcaacta
    87421 tattatcttt taaagcaatt gaatgaggat atcctcttat catatcaaat tttaaagcat
    87481 taaaagcttc tacagctttg tttgagtaag acacggtatt tataccttca ccacagtcta
    87541 tctcgaatac atcttctgaa tgtccagatt ctattgcgta actttttttc aactgcattt
    87601 caatagcttt gttaattttt agtttcatac tttatcactc cttatttagt caataataaa
    87661 tgttataaaa gttgctagat ttaaaatatt atgaaaatat ataatagtaa aaaatgaata
    87721 taaatattat aatagctcac tttatttaat atgattatat atcatatatt gtaaaatttc
    87781 tagcattata agactataaa ttgaaaatat tcagaaaaat actacaagaa acgatttatt
    87841 ttgctcttaa aataagttaa atgaatgtta ttaattttgt aaattgaaat agtttaagta
    87901 taaagttatg atataatcta ataagggtaa ttctttagat tatattaagg aaatataggt
    87961 atacaataaa aagttatgga gttggattat gggatataaa ttaattgtaa cagatatgga
    88021 tggaacttta ttaggcaata atcataaagt tacagatgaa aataaaactg cactacaaaa
    88081 agttataaaa agcggtatta atgtaacttt agcaacagga agagccttcg attcagctaa
    88141 atgcaatgta gattttctta aagaggacat gccaataata gcttgtaatg gttcattaat
    88201 aagagagcaa aatggaaaca taatatattc aaataagatt gatacaagaa catgtttaaa
    88261 cattttagat gtattagata aatacgatat ttactaccaa tgtaatagta tagattctat
    88321 gctttcgaaa aaaatagaag gacgtgaaga tagattaagt gtatttttag gctctgaaac
    88381 agaagtaatt gtaaaagatg atttgagaga agaaatcttt aaaaaagata tactaaaatt
    88441 tgtaatcata gaagaaaaaa atccatctat tttggatgaa ataagaaaag aattgagaaa
    88501 agttcaagga ataaagataa cttcttcatg gccaaacaat attgaagtca tgaatgaagg
    88561 tgttgataaa ggtaatgcag ttaaaatttt agctgaaaaa atgaatatag acagagaaga
    88621 tataattgca tttggagaca attacaatga catagaaatg ataaagtttg ctggtcttgg
    88681 ggtagctatg ggaaatgcag aagaattgat taaacaagaa gctgattatg ttacagatac
    88741 aaatcaagac agtggagttg caaaggcaat ttataaattc ctagagttaa aagaatcact
    88801 tagctcttaa aacagtatat aataggaaaa aataggttga ctattttgta gatagtaatt
    88861 ataatacaaa tagataaaac ataaaaattt agattcagtg aaaaagagag tagatataga
    88921 gaaaatctca gcgaattggg ggatggtgtg agcctaatga taacacttta tattgaaaag
    88981 aactttggag aatactactt gaaattcagt atggtagtag gtaatctcgc cttaagagaa
    89041 tagagtgggt aaacattgtt taccaactag agtggtaaca cggaatattt cgtctcttgg
    89101 tctttttgac caagagattt tttagtttca ttgagttaca agacttataa ttaaaataca
    89161 aaaaattaaa atatgaggtg aaagttatga aattatgggg cggacgcttt agaaaagcgg
    89221 aaaatcaact tatggaagaa tttaataaat cttttggcta tgattgtgta ctgtacaaaa
    89281 aagatatcga aggaagtgta gcacatgttc atatgcaagt aaaatgtgga cttttaacag
    89341 aagaagaagg aaagtctata acagaaggtt taaaagggat acttgaagat gtagaaaatg
    89401 gaaagttagt attggatgga gagtatgaag atatacatag ttttactgag ataaatttaa
    89461 ttcaaagaat tggagatgtt ggcaagaagc ttcatacagc acgtagtaga aatgaccaag
    89521 ttgcagttga tatgagattg tatgctaaag aaaaagcaaa tgatttggtg ggtttaatat
    89581 ctgaatttaa agctactata aaagatgttg ctgacaaaaa cccagtgatg atgccgggct
    89641 atactcatct tcaaagggca caagtagtga catttaagca tcacttaatg gcatattata
    89701 gtatgtttga tagagatgag aaaagaataa aaaatgctat agaaatatta gatgaatcac
    89761 ctttaggctg tggagcttta gcaggaacta cgcatgatat agatagaagt ataacttgtg
    89821 aacaattagg atttaaaaaa gtagtagata attttatgga tggagtaagt gatagagatt
    89881 atttactaga gctaatgagt gatttttcaa ttataatgat gcacttaagt agattaagtg
    89941 aagagttgat actttggagt tctcaagaat ttggatttgt tgagatagat gatttatata
    90001 caactggaag tagtataatg cctcagaaga aaaatccaga tggtgcagag cttataagag
    90061 gtaagactgg tagagtatat ggaaacttat ttggtctttt tacagtaatg aaaggaatac
    90121 cattagcata taataaagac atgcaagaag ataaagaagg ttttttcgat agtgtacata
    90181 ctcttgaaat gtgtatacaa ataatggata gaatgatagc cacactaaag gtaaatgagg
    90241 ataaaatgaa acaagcagta aaaaatggtt ttttaaatgc aacagaagtt gcggattatt
    90301 tggttaaaaa taatgttgct tttagagatg cacatggtat agtaggaagt atcgttatat
    90361 attgtgaaga taataaaaaa gcaatagaag atttaacatt agaagaatta caaaagtttt
    90421 cagatgcttt taaggaagat atttatgatt ttatcgacta tgagagtata ttaaataagg
    90481 gaataaaaaa gaatttaaaa taatgtattt atttttaaaa gctgtaaatt tgaatgttat
    90541 ca
//
LOCUS       DAGSRM010000006        88075 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.006, whole genome shotgun sequence.
ACCESSION   DAGSRM010000006 DAGSRM010000000
VERSION     DAGSRM010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 88075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 88075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..88075
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.006"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            223..1491
                     /gene="eam"
                     /locus_tag="MJE33_000455"
     CDS             223..1491
                     /gene="eam"
                     /locus_tag="MJE33_000455"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HBY9646432.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEYIKEVGTQFRWAISPY
                     YLSLIDPEDICDPIKLLSIPTHIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPIYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(1584..1940)
                     /locus_tag="MJE33_000456"
     CDS             complement(1584..1940)
                     /locus_tag="MJE33_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646433.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTSEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(2091..3185)
                     /locus_tag="MJE33_000457"
     CDS             complement(2091..3185)
                     /locus_tag="MJE33_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBY9646434.1"
                     /translation="MQDKKIYLNIVLTVVVSYILIKVIDNYKYFFGVISLLMSLLTPF
                     IIAFVLAYIFNPLVKFLESKLNFKRIYSLLLTYGVLIVILISFILFTVPSIVNSLADL
                     VAQIPTYIDKTEQFLFDLGKSLQSVDPNTLKEYGDKIMSVMPKFSNLLIGSLGGIFST
                     TFSVGKFIVQFLLGFIICFYILLEKEKFLLFTKKVVYISLGKKYGDFIIELCQSLNLN
                     IGKYFTGKILDSFIVGVLSGIGLYFLKSEYALLFGTLMGVMNMVPYFGPVIGMAPVVI
                     INLFSNPTIALTSLIYLIIIQQVEVTFIEPKIVGGQLGLSPFFTILAVTVGGGFFGIP
                     GMILSAPVMGVIKIYFCEFVNRRHDKIQME"
     gene            3875..6922
                     /locus_tag="MJE33_000458"
     CDS             3875..6922
                     /locus_tag="MJE33_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBY9646435.1"
                     /translation="MKKILKRLCTGFLAFATVVTALPSSTVHASNTQYWTESKERVGI
                     VEQVMNDGSISSTFNEGHLTVEGKDAYCIDINTAFKNGYKTRTDASSRMSADRISDVA
                     LSIEYVKQYTDSHSGISSKHAYLLKQLVVWQRLSVQLDWQCDNVRASYDEIPKAVQDE
                     VFAGAKAFVKENKGRYDCGGYIYSGEGQELGQFWAKLAVGNAKLQKTSTNANITDGNG
                     IYSIAGATYGVYSDKDCTKQLATLTTDTSGNTEAVEVRATTVYIKELSAPAGFKIDKT
                     VYSLSVEAGKTATLKVSDTPKVTDTLIELFKIDMETQKSNSQGNASLEGAEFTWNFYA
                     GYYNKNNLPAQPTRTWVTKTIAEKDSDGAIHYITRLANAYKVSGDSFYMQDGKNVLPL
                     GTLTVEETKSPSGYLLEGAYMQADGSEEQIKEMYLTQITEDGDLAVLSGSNQYHVSDK
                     VIRGGVKIQKRDLETKDTKAQGGATLKNTAFEIISLNENAVLVESKLYKKNEVLKTIH
                     TDIEGIASTSADLLPYGKYRLSEQKPPEGYLTDGAKPIDFEITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGAGSHKRLADVPFRITSKTTGESHVVVTDDNGQFSTASDWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALIYDTYIIEELRCESNKGFKLIPPFEIVISRN
                     KVVVDLGTLTDEYEKEITIHTTATSKDGEKTILAGNDVTIIDTVKLDGLVKGTKYQLK
                     GWQMLKKENAELLVGDKRVESDYTFVADDEAMKVEIAYTFNASALGGKNLVTFEELYD
                     LSNPEEPVKVAEHKDIEDDGQTVLITERIIKIHTTATDKDGKKEIEAGKDVTIVDTVK
                     LEGLEVGTKYQLVGWQMLKEENAELIINDKKVENDYTFTSDSETMEVKIAFTFDASSL
                     GGKQFVTFEELYDLSNPDKPIKVTEHKDIEDDGQTVTIKEVPETPTPEEPEKPTTPDT
                     PTKTDSPKTGDNTNILAFAIMMFVSAGGLAGTYFFKRRKMKKS"
     gene            7144..7467
                     /locus_tag="MJE33_000459"
     CDS             7144..7467
                     /locus_tag="MJE33_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBY9646436.1"
                     /translation="MELKYIVPNVAQTFGNLEFAGENKVDQQHINGRYTPVSRSYNLY
                     SDIQRADDIIVVLPAKAGEKHFKPEQKVKLINPRITAEGYNVKGRGFTNYILMADDML
                     PAESK"
     gene            7482..7895
                     /locus_tag="MJE33_000460"
     CDS             7482..7895
                     /locus_tag="MJE33_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBY9646437.1"
                     /translation="MRLANGIVLDNEKTFGVLKFSALRHEVRVENEDGTTSDEIKRRT
                     YDLKCSVQERMIQVSIPADVPVKDFPYNAEVELINPVADTVANANFRGADVDWYIKAD
                     DIILKNKGNQPAGNPQNNNPQNLAPQGQQKNKLAN"
     gene            7976..8434
                     /locus_tag="MJE33_000461"
     CDS             7976..8434
                     /locus_tag="MJE33_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBY9646438.1"
                     /translation="MKFKNPMLVVTDIDKSVEFYKKVFGLRVIMDFGANKTLTGGLAL
                     QTSETYKEFIGTSDISFGGKNFEVYFEEDDFDKFADRLKEYDIEYVHPIIEHSWGQRV
                     VRFYDPDKHIIEVGENMKIVCKRFLNSGMTPEQVAERMDVPMKFINACMR"
     gene            8498..8911
                     /locus_tag="MJE33_000462"
     CDS             8498..8911
                     /locus_tag="MJE33_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBY9646439.1"
                     /translation="MLDKIPNAEEMTALVGQSLYAIWNKLCALIDEKYDMECLWNKGG
                     KAWTYEYKYRRGGKTLCALYARENCVGFMIILGKDERLKFDTDRNSYSKEVQKIYDET
                     KTYHDGKWLMFEPTDTILFNDFVKLLGIKRKPNRK"
     gene            8966..9568
                     /locus_tag="MJE33_000463"
     CDS             8966..9568
                     /locus_tag="MJE33_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBY9646440.1"
                     /translation="MDKVVIYIHGKGGNAEESSHYKPLFSDCDVVGLNYTAQYPWEAK
                     EEFPSLFDLICKHYKSVQIVANSIGAYFAMNALSDKQIEKAYFISPIVNMEKLIADMM
                     YWANVTEDELRDKKEIKTTFGETLSWEYLCYVKENPTTWKIPTNILFGEKDNLTSYAT
                     ISEFTKQTNATLTVMRNGEHWFHTEEQMKFLDEWINKSAR"
     gene            9629..10171
                     /locus_tag="MJE33_000464"
     CDS             9629..10171
                     /locus_tag="MJE33_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBY9646441.1"
                     /translation="MKGFERKNQLFSLCGLNCGLCPMLLGNYCGGCGNGNQSCRIAKC
                     SLEHGQIEYCYECKQYPCEKYQHIDDYDSFITHKQRKADLERAKNIGIEQYNLEQQEK
                     TQILSYLLSNYNDGRRKTFFCVAVNLLELSELQEAMKQIQSNDELTLLPFKAQCLYVV
                     EVFQKIAERRNIKLKLFKKK"
     gene            10199..10510
                     /locus_tag="MJE33_000465"
                     /pseudo
     CDS             10199..10510
                     /locus_tag="MJE33_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896960.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
     gene            10621..12015
                     /locus_tag="MJE33_000466"
     CDS             10621..12015
                     /locus_tag="MJE33_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBY9646442.1"
                     /translation="MRIIWNKGHRIRASDKNLVYRFSLGTLLIVFVVVLLLLNMKQLM
                     RTDWEHFSLLDNSFTLSLYNFITLLIATGVCALVAFLYYRFCYDNFKKLLHRQKLARM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIVWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIKYILLYDMIANRITIDEVRAENGCLRLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEEMIDCVNAFYEGMVQRSEEMKQHPNYKTGENYAYLSLPPCFLIFDEYVAFFEML
                     GTKESVSLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSIISEFYTPLVPKGYDFLQTIGSLAYARQ
                     DGTATCEAKGDGTD"
     gene            12217..13416
                     /locus_tag="MJE33_000467"
     CDS             12217..13416
                     /locus_tag="MJE33_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBY9646443.1"
                     /translation="MNETDFLTALKAKRCAYSVSQTRLAIMANISREHLSRIEAGKVT
                     LTEDMKHTLLEAVEKFNPDNPMFLLFDYVRIRFPTMDIKHIIKNILKLNIDYMLHEDY
                     GHYKYTEHYHIGDVFVYVSQDEEKGVLLELKGKGCRQFESYLLAQERNWYDFFTDALV
                     EGGVMKRLDLAINDRTGLLDIPELIQKCENEECISKFRSFKSYGSGELVKHNETDKYG
                     MGHTLYIGSFSSEVYFCCYEKNYEQYAKLGIPIEEVPIKNRFEIRLKNERAYYAVVEL
                     LTHYDAEQTAFSIINQYIRFADKEPDKRKSDWKTNVRWAWFIGEGRPPVKLTTKPKPY
                     TLERTLNWLQRQVAPTLKMLKQIDKDNQTEYLETIEKQAKLTERHHQIIKQQTATVEE
                     MVAKVKE"
     gene            13423..13557
                     /locus_tag="MJE33_000468"
     CDS             13423..13557
                     /locus_tag="MJE33_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBY9646444.1"
                     /translation="MWELLKDFLLVSLGMGIGVVLMCILTVGKEADRCMEEIKEREEH
                     "
     gene            14299..16209
                     /gene="ltrA"
                     /locus_tag="MJE33_000469"
     CDS             14299..16209
                     /gene="ltrA"
                     /locus_tag="MJE33_000469"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBY9646445.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGTPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMPDGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWHENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKTKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKTQKDKLVSQAKKI
                     ASPSKGKTELEEIRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGASKKG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYSANCYTPEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTRAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            16376..16537
                     /locus_tag="MJE33_000470"
     CDS             16376..16537
                     /locus_tag="MJE33_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646446.1"
                     /translation="MAIVVIGIYLGFKREFSKLIGFLVVALVAVGLVFNAGGVKDVLL
                     ELFNKIIGA"
     gene            16618..16995
                     /locus_tag="MJE33_000471"
     CDS             16618..16995
                     /locus_tag="MJE33_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY9646447.1"
                     /translation="MDYKVDDKNLDASMFISFVNQVWQGDYDMEQTRQALSKTLNITA
                     YDNKKLVGCLRILSDGYYFGTITELLVLPQYQKQGIGSKLLQLAKDNTPTMLYFGSQP
                     EAEKFYEKNGCQKSLQSYMINKG"
     gene            17102..17398
                     /locus_tag="MJE33_000472"
     CDS             17102..17398
                     /locus_tag="MJE33_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBY9646448.1"
                     /translation="MNDIFKNMQAKIGCEYLSDLPSYKRKVWHEMKQLNLADYEEKQL
                     EDFSKYVFGMSYQTLKDVMKQQIGREEQCRKQGYWWKRKEQLTKKQHHTGSTCR"
     gene            17317..17820
                     /locus_tag="MJE33_000473"
     CDS             17317..17820
                     /locus_tag="MJE33_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBY9646449.1"
                     /translation="MQETRVLVETKRTTDEETTSYWFDLPIEVAEFEEKLGVGAESGE
                     YRIIEKVLPYADEVHEHTSVYQLNKLDFMYRQLSSDMQEEYVSLLMVFENLEALYICR
                     NVITVYPDCKSMIDVARQKLMNDPTFKHLSEDCQEYYFNFEAYASHLQEHGKFLVTEH
                     GIFELPE"
     gene            17838..18341
                     /locus_tag="MJE33_000474"
     CDS             17838..18341
                     /locus_tag="MJE33_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBY9646450.1"
                     /translation="MIDDMAVYIANLGKYNEGYLVGAWFSFPIDEEDVKEKIGLNEQY
                     EEYAVHDTDNFPIDIGEYVSIEELNEMYEMIEELPDYIVECLDEFISHYGTLEEVVEH
                     KDDIYYYPDCETMTDVAYYYIDELQALGDIPPSLQNYIDYEAYGRDLDMGGCFIETSR
                     GMCEIPY"
     gene            18462..18860
                     /locus_tag="MJE33_000475"
     CDS             18462..18860
                     /locus_tag="MJE33_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBY9646451.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDMPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLHKQTLNETITAVRSVNYVPDKIY"
     gene            18838..21288
                     /locus_tag="MJE33_000476"
     CDS             18838..21288
                     /locus_tag="MJE33_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBY9646452.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIRSMQEQSKKLVTGKLREVAVQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNIKKSAWLTFTEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEINDFGYLMEHLYGRDGIAYEDYEYQLPEKKLQKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALSTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQSKESMYKLSYVIRVSAPDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGENTGIYMGYSVDTGRNVYLQPS
                     LASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYYSVLFGGQAVILDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKDNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRSVTQSDSRGLLHVIDELRREDTPISRNIADHIDSFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSAYDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKEDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPVQRNEVE"
     gene            21288..23483
                     /locus_tag="MJE33_000477"
     CDS             21288..23483
                     /locus_tag="MJE33_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBY9646453.1"
                     /translation="MKERIQSAFTKKKMFRFLKITVFVVALSLILLSLLETVAHATGL
                     VDDTINTENLYSKYPLSNYQLDFYVDNSWAWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGQSIQTLAGVTSSGFSTSGFYVGFLLLIIL
                     VVGIYVAYTGLIKRETSKALHAVINFVVVFILSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVEQPWLLLQFGNSNAEEIGTDRVEALV
                     SVSPEDEDGKTREEIVKTEIEDNDNNILTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNASDSQSMGRRIFRRP
                     YLFIRHRARRMERRMARAVSAGGISGGVAGALAGSTVSNARTTRNNPTKENRGNTTSI
                     MGQRAGSKIGAVLDTKNKVKDKANAVKENVKDMPTQTAYAVYSAKEKAKSSVSDFKRG
                     MVQEQQSRQTGRLEKQEQRKKNIANKRMELQKAQEARQARKADGSATTGATRPHERPA
                     TASKPSVEKVQEVKRPATTPTSKASEPVKTNVIKERPLSSGASDGKATQSAQPIHRQN
                     IEKVVSQETRQNYVKDRRTKVQQTQSVQKNQQTTEKNHNLVTKKGQKKK"
     gene            23480..24484
                     /locus_tag="MJE33_000478"
     CDS             23480..24484
                     /locus_tag="MJE33_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBY9646454.1"
                     /translation="MKLKHIALIGSLFPILFSIVLFFGVLISADSDDENSNFSSGITG
                     LNLSAEVLKHQPMVEKYAREYGISEYVNVLLAIIQVESGGTAEDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSYKNQGINDLNVAIQSYNYGGGYVGYVAGKGKKHTFTLA
                     ESFACEKSGGKKVTYTNTIAVAKNGGWRYGYGNMFYVELVNQYLTVPQVSGELAQKIM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLVQWCYGKAGISLPRTAQAQYDATQHIPLSQA
                     KAGDLVFFHSTYNAGSYVTHVGIYVGNNQMYHAGDPIGYADLNSSYWQQHLIGAGRVK
                     "
     gene            24504..25406
                     /locus_tag="MJE33_000479"
     CDS             24504..25406
                     /locus_tag="MJE33_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBY9646455.1"
                     /translation="MFKKSKKQTENVKEKKVRTVKVGTHKKTVIAFWVVLIVSVSFGV
                     YKNFTAIDMHTVHETETIQLRLTDTNGIENFVKNFAKSYYTWNNSKEAIEARTQAISG
                     YLTKELQDLNVDTIRTDIPTSSTVTDVIVWNIEQSGTDTFSATYEVDQQIKEGEQTTA
                     VKAIYTVKVHVDVDGDMVIIQNPTLAPTIEKSDYELKTPEADASVDADTINDATSFLE
                     TFFKLYPIATEKELAYYVSGNVLEPIGKDYLYSELINPVFTKDGDNVKVKVAVKFLDN
                     QTKATQVSQYELVLHKDSNWKIVK"
     gene            25550..26161
                     /locus_tag="MJE33_000480"
     CDS             25550..26161
                     /locus_tag="MJE33_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY9646456.1"
                     /translation="MPKGIMLTQEQQEERREEIISVALQLIEKNGFQKTSMRKIAILA
                     NMGKSSLYDFFKTKDEIFVYAVEKEIEETIKKVHRIIADESSPEQCLRKIMLNHLGVP
                     KQYRTVLMWLNTESDYLEEDYRKRLKTARYAYQDIIKSVIENGMESGVFRKTNANLMT
                     RLLINSIISIIYTSRPSASPEKMLDETMDIFLHGIMNDGGELL"
     gene            26158..28725
                     /locus_tag="MJE33_000481"
     CDS             26158..28725
                     /locus_tag="MJE33_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBY9646457.1"
                     /translation="MIYYTLPLLHKQATLEMVGGKGMSLSKLLTTGIPVPEGFHVTTT
                     SYKIFVEKNHIQPYINKLLDGIDTNNTSQLENVSTQIGMLFHNGEMPQEVSDAIKTAY
                     ARLGNIAVAVRSSATAEDLPDASFAGQQETYLNIQGENEVLDAVKRCWASLWTARAIA
                     YRVKNDIKQEIVALAVVVQKLAFSDASGVMFTLNPINGRRSEMIVNAAWGLGESVVSS
                     LVTPDTIVVDKNAERIVSYEVANKEIMTVRNSDGTEEIPTPEQLRKKHALTRNQVMRL
                     TQLGKKIEKYYEMPMDVEWALEKDKLYIVQARPITVLPPEWTLPEKDVIYTRGSLAEH
                     LPNPVTPLFATLGLEIVNRASALLWIDMFGKSAKKLLPENGAYTIINGYVYLSANSKP
                     LLIAVKSLSPRSLRRALTNSVARWETARKEFEDVIKQWEEKPMHMLNAHQIMEGIQTV
                     FYGACIYFTRIQLTLPAASISETLFTKFFQGAARRAGMTDTSVFLLGFDTIALQAEKN
                     LWSLSEWVKQNNTLNLYLQSNPTAKIAENFMSSVPPDEVSLEVWIEWKNRINQYFKEF
                     GRTAYEFDFAYSTPQETLTPTFESIKTFVEGKGESPFLRQSKFEKHRKQAEEEILQHI
                     GSSRKKLFLKLLHWAQETAPMRENAIYCMGMGHPLIRQMFHEISARLIRGGATSHIDD
                     IYWLTKLELEKLIVQLDKNIPLSDRRNSILARKAELKKYQGYMPPAQLPEKKVKSISH
                     APQKQKDGKTVLRGIGTSSGVVTARACVLNSPADFKNFQPGSVLVAVTTTPAWTPLFA
                     SASGIVTDIGGPLSHSSIVARECGIPAVMATHTATRSIKNGQMITVDGSEGTVTIDE"
     gene            28718..29878
                     /locus_tag="MJE33_000482"
     CDS             28718..29878
                     /locus_tag="MJE33_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY9646458.1"
                     /translation="MNKKPIFALNLATLVIALGYSLILPVMPFYMENLGAGGRELGWL
                     TAVYALAQTVCAPFWGALSDRIGRKPIISIGIIGYSISLFLFGLASSFWTLFIARSLS
                     GILSSATSVASLAYVGDSSSEEERSKNMSQLGASMSVGVMMGPLLGGILAGYSLALPF
                     FTGAGISFIAFLLILTLLPESIERSGQTEKRKPFDFSDLKSIIFGSAGMVLILIFVVH
                     FCQTGLQGITGLYVVDKFGFSTKQVGVIWMVLAGILIVVQGFLTGPLAKKIGEKRLIL
                     IGMFCKALVAFAMVLTTGFVSVLVVFGLFAVSSAITVPTLNATLSKTDNQHKGTLMGF
                     ASTAGNLSKVIAPLLTGYLYESNIELPYITTGGIALIGVVICYIWIKNQKNK"
     gene            complement(30065..30258)
                     /locus_tag="MJE33_000483"
                     /pseudo
     CDS             complement(30065..30258)
                     /locus_tag="MJE33_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            30361..30867
                     /locus_tag="MJE33_000484"
     CDS             30361..30867
                     /locus_tag="MJE33_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBY9646459.1"
                     /translation="MKNEKKITVALLIVYLLILSWIILFKLQFSFSVLDHIRQINLIP
                     FGGSVIANGKIDFDEIINNAIVFIPVGAYFSLLFKNKSVLKAIGSVLGISFVYEIIQF
                     IFAIGASDITDLISNTLGGIIGIALVYVLSIVLKDKTHKILNRIAIVCTVLVVAFLFM
                     LLAVNNIF"
     gene            30872..31147
                     /locus_tag="MJE33_000485"
     CDS             30872..31147
                     /locus_tag="MJE33_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4865 family protein"
                     /protein_id="HBY9646460.1"
                     /translation="MLITCCFGKDFNLSKFAFEIEHEITPMEQMKAINFSLEKDDCTS
                     KVLIYNPDKWKYVEYYFYKDRPIKKLLEKFIQFCICHNKICFEILNT"
     gene            31417..31989
                     /locus_tag="MJE33_000486"
     CDS             31417..31989
                     /locus_tag="MJE33_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBY9646461.1"
                     /translation="MNVDTIRTDIPTSSAVTDVIVWNIEQSGTDTLSATYEVDQQIKE
                     REQTTAVKATYTVKVHVDADGDMVIVQNLTLAPAIEKSDYEPKTSETDASVDADTVND
                     ATAFLETFFEVYPTATEKELAYYVSENVIEPIGRDYLYSELVNPVFTKDGDNVKVKVA
                     VKFLDNQTKATQVSQYDLVLHKDSNWKILG"
     gene            32092..32907
                     /locus_tag="MJE33_000487"
     CDS             32092..32907
                     /locus_tag="MJE33_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBY9646462.1"
                     /translation="MFKIGEFSKLTQVSTRMLRYYDETGLLKPAKIDSLTGYRMYSAE
                     QISTLNRIIYLRDSGFNVAEIALALNFSNNAIIEQLDRKSIEIMDNIQKEQEKLKKIE
                     IAKKEVLHGKSELYFNITIKEIPSYCVLSLRKEIPNYYYEGELWKELSVFAKENKINI
                     SDETFSIYHNEEYKEKDVDVELCAIVNKIGKATGDFKFRFTEPVSMMACTMVYGDFFN
                     ISYAYRSFAKWLQENSQYKMKGSSRQIVHRGPWNEKNPENYLTEIQIPLENLL"
     gene            32988..33464
                     /locus_tag="MJE33_000488"
     CDS             32988..33464
                     /locus_tag="MJE33_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBY9646463.1"
                     /translation="MITYKVNSIGTIYNNEKGAFIQLEAAYIPALRALDGFSHINVLW
                     WFSDFDNTDFRSVLETEQPYKNAPEKMGIFATRSPVRPNPIALSVVEIINIDYMNGMI
                     QIAYIDANDKTPILDIKPYTPSLDRVENPNVPDWCSHWPHSLEESASFAWENEFNF"
     gene            33478..34029
                     /locus_tag="MJE33_000489"
     CDS             33478..34029
                     /locus_tag="MJE33_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY9646464.1"
                     /translation="MSLQDYIIEKPVIDTEHLILRTMDKNDIDDLKEWISDISLYEYW
                     GKRPSKSEKNPEVLFSKPEKATKSFHWGIVHKQDSKVIGEIWIYLIENNRMAKVAFRL
                     SKQYQGNGLMSEALKSVIEFCFSKTELQKIWTDVHILNHASYKTLEKVGFQREGHIRG
                     GKMVNTYCDYYLYGILKSDYITS"
     gene            complement(34419..35588)
                     /locus_tag="MJE33_000490"
     CDS             complement(34419..35588)
                     /locus_tag="MJE33_000490"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBY9646465.1"
                     /translation="MFYIGIDVGKFNHCACVISSEGEVLIEPFFFTNNLDGFDSFIKA
                     VKKFKSPRHIVGLEATGHYGDNLINFLITHDYEVALINPLTTDAKRKSKIRKTKNDKK
                     DTFLICSVLFDRDYTRVTQRKLDMKQARELTRYHASIAEDINQKKNQLQKCIDIVIPE
                     FNTWFKTKYSKAYMAVLKEFGSSYNLASANLAHLKKVLKPKSSGSRVNIDASSLRSSA
                     KKSIGENNEAVTLEIKMLIENIELLASQLKTVDKKIEELAVKLDSPIFSIPGIGIYTG
                     MSILSEIGDIHNFSSAVKVMGFAGVDPGTYQSGEYSAPRTALSKRGSRYLRKSLYQCI
                     LTVCKYNLTFNKYYKLKRLQGKSHRCAQGHSVRKLIRVIYKLLSENIQFDEKLLI"
     gene            35867..36778
                     /locus_tag="MJE33_000491"
     CDS             35867..36778
                     /locus_tag="MJE33_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBY9646466.1"
                     /translation="MYAYENIEMSLNYIEQHLSEKIETEKLAEIACLSTFYYQRLFKK
                     LVKKSVQEYIKLRRLAMVIAELNNSEERILDIALKYGFSDHANFTRAFKEVYHITPDE
                     YRKNVPMLNTFDKPELSSRYIMIDENVPLIVGNIVLEIQRKTLRTKETYFGFEKTVNI
                     AEQIPAGESTGIDVPGQLWREFHAKKLQTENAIFNNDIELGVSHLASPDKGTFLYFAG
                     GMVSSTDVCPKNMVQHTLPAGEYIVCKIEAESFEELVTTALNQANKYLFETWLPNHNL
                     VTKPFMAEKYYKGNAEINYMEVWIIPV"
     gene            36856..37290
                     /locus_tag="MJE33_000492"
     CDS             36856..37290
                     /locus_tag="MJE33_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBY9646467.1"
                     /translation="MINIKDINYVPNISEISVYIENSAFDELYQHMNNEYKAVCKIEY
                     SKDVWARGWNVKFKKSGKSLCVVYPKKNYFTVLVVVGNKEKEDVENLLPRLSEYVQEI
                     YRNTREGNGQRWLMIDLKSKNNVYQDVLRLIEIRYAIKCVKP"
     gene            37758..38498
                     /locus_tag="MJE33_000493"
                     /pseudo
     CDS             37758..38498
                     /locus_tag="MJE33_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            38449..39060
                     /locus_tag="MJE33_000494"
                     /pseudo
     CDS             38449..39060
                     /locus_tag="MJE33_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            39273..40130
                     /locus_tag="MJE33_000495"
     CDS             39273..40130
                     /locus_tag="MJE33_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBY9646468.1"
                     /translation="MYKEVGISSNNVPITLSIWYTERSSPTIVFLPGTMSHPLMYENF
                     LCGLSERGFNIIGIHYLSHGKSPKIKKNYTMEDLLHNVYDATTYGIENFGENIAVMGS
                     SQGGILAAMAAGRDTRLKAVFPHNIMLTTLKETMSLTKYPTWSHRFMGLIQWGFRVGG
                     KLLPNYKVPGDAYLDYDRVFYSEQAKQDCLNDPMLLPYYPLRFVASLFNADLSCLENG
                     AIQCPVILITAKGDPLFSFDYTNKVFDLIHVPQKELLALELNRHMIFNEDTDTTINAL
                     EATLHKYLG"
     gene            40208..40789
                     /locus_tag="MJE33_000496"
     CDS             40208..40789
                     /locus_tag="MJE33_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY9646469.1"
                     /translation="MPRFTEQEKEIINSKLLIEGEKLFALHGLKKVTVDDLVAAVNIS
                     KGSFYAFYPSKEHLYVEINFRLQKELFTSIETTIKKKKYKNHRDLAKDVIMLSLTGVI
                     TSPILSQIDLSLMDYLQRKISSDIFENHMYSDIRILEILEDLGVVFLVPHTVIIKSLY
                     SVLSCLEQFKEDEELNMIQNLLVNGIIQQVVAE"
     gene            40893..41738
                     /locus_tag="MJE33_000497"
     CDS             40893..41738
                     /locus_tag="MJE33_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9646470.1"
                     /translation="MIDVKKLYFSYTDKPFVENVSFHVGRGEIFGFLGPSGAGKSTIQ
                     KVLTGLNTRYKGSVKVAGTEIREHTNQFYENIGVDFEFSTCYEKFTARQNLAYFASLY
                     EKQPRSIDELLHMVGLENDGDKKVADFSKGMRSRLNFIKALVHDPDILFLDEPTSGLD
                     PTNSRLMKDIILAEKKRGKTIIITTHNMFDATELCDQVAFIVAGKVSALDSPHNLIMS
                     RGAAKIQYTYYDKGEKTGECLLDRTTEDKLLKSLISENRLLSIHSSEPTLNDIFVDIT
                     GRTLQ"
     gene            41735..42418
                     /locus_tag="MJE33_000498"
     CDS             41735..42418
                     /locus_tag="MJE33_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9646471.1"
                     /translation="MRLGRLICGDIHFQWKYGFYFIYFILTVLYVCGIAALPEHWKTD
                     IASIMIYSDPAAMGLFFMGAIVLLEKSQKVLNAMVVSPVKVSEYILSKTIALIAISTV
                     IALILGVVSDSNHLLGITVGTALTSAIFTMLGIIAATKISNLNQFLIVIMPIEIVCFV
                     PPIVGLFVKLPYLFRFFPFTACMNLITGKSVLLSFDMVLVIATLIILYIVARHTVKHM
                     WKSLGGVKL"
     gene            42415..43113
                     /locus_tag="MJE33_000499"
     CDS             42415..43113
                     /locus_tag="MJE33_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9646472.1"
                     /translation="MKRILSSTIQVFRQIKSDPMMFAACFTPFVMGALIKLGIPFLER
                     ITDFSLQAYYPIFDLLLSIMAPVLLCFAFAMITLEEIDDKVSRYFSITPLGKSGYLFT
                     RLGVPSIISAVIAFIVLLLFSLEKLSIGMTICLALLGSVQAIIVSLMIITLSSNKLEG
                     MAVTKLAALTLLGIPAPFFIDSYYQFAVGFLPSFWVAKAMQNEAVLYFSIGLVVALVW
                     YYFLTKRLFRKLAG"
     gene            43125..44036
                     /locus_tag="MJE33_000500"
     CDS             43125..44036
                     /locus_tag="MJE33_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBY9646473.1"
                     /translation="MEKGRLLQNGKVNRAIQVSKITLFINLSLSLLKFTAGYIGKSSA
                     MLSDAVHSASDVLSTIVVMVGIKISEKQPDREHPYGHERMECAASIILSVALAITGAG
                     IGYSGIKKIFSEQYNTLSTPSGIALTAAVLSIVIKEWMYWFTRNAAKHTNSDALMADA
                     WHHRSDALSSVGSLIGILGARLGYAILDPIASVVICGCILKAALDIFKESINKMVDHS
                     CDNATETKIREVVLQQQGVDGIDELKTRMFGAKMYVDIEILADGNLALYDAHRIAEGV
                     HQTIENNFPQCKHCMVHVNTNELLYSA"
     gene            44088..44228
                     /locus_tag="MJE33_000501"
     CDS             44088..44228
                     /locus_tag="MJE33_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646474.1"
                     /translation="MEQYWWIIIVVVLIVVGLLKIVIMKKYNQRRNSNNSPKDSEDDK
                     MY"
     gene            44518..45144
                     /locus_tag="MJE33_000502"
     CDS             44518..45144
                     /locus_tag="MJE33_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY9646475.1"
                     /translation="MSDTKENILKIALHLFAVNGYEAVSVSMIAEALGMTKGALYKHY
                     KNKQDIFDCIVERMYQIDNKRAKDYQVPESTIEVTPNAYRNTTIGSLITFTLAQYDFW
                     VTDEFASNFRKMLILEQYRNLKVNMLYQKCLVNGPISYIEDIFNEMIEKGVLQECDTK
                     QLAIEYYAPLFLLISVADAVENCWDLKTQLTTHVKQFVKKYIINEKVK"
     gene            45266..45982
                     /locus_tag="MJE33_000503"
     CDS             45266..45982
                     /locus_tag="MJE33_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBY9646476.1"
                     /translation="MEYIRSKKYFTPDLQTKIMGPNPIKLQEELLQNHKIPSGSYVCD
                     LGSGQGLTSVFLAKEYGFVVFAVDLWSEPKENEEFFKKMGLSSAQIKSVKADANELKF
                     EKEFFDAVVSTDSYNYFGRDPIYLDEKLLPFVKKGGFVYITVPGMKKDCHEKLPEELL
                     LSWTPEELDYIHDITYWSNIISHCKKADLISIYEMESNSEVWSDWIKQNNEYAINDRK
                     AIEAGGGDYLNFIAIILQKK"
     gene            46146..46337
                     /locus_tag="MJE33_000504"
     CDS             46146..46337
                     /locus_tag="MJE33_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HBY9646477.1"
                     /translation="MKKYEYVEINYNAKGVVFLCTDKHKEIIDSYAKTGYHYAGFVPT
                     EIDAKGCMRKIDLIFEKED"
     gene            46361..46591
                     /locus_tag="MJE33_000505"
     CDS             46361..46591
                     /locus_tag="MJE33_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646478.1"
                     /translation="MIGLKRRNMNYIMKVGAKAQGVSVSEFRAMLQDTIDKTMESTDP
                     DVQANFKRYFGNKRPTPEEYIYTITKKTKVKV"
     gene            46858..47277
                     /locus_tag="MJE33_000506"
     CDS             46858..47277
                     /locus_tag="MJE33_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBY9646479.1"
                     /translation="MKPSDFQKTIQCQFECKLKKVVKGIIWNYRKELKRRKNKEISFS
                     ELPDITVESLATWDDYETNYTIFNVCGIDIKVLDDELAEALKQLPEKKRNNVLMYYFL
                     EMSDTEIADIMNYTRGAVFKHRHRALETMKEILKEKK"
     gene            47280..47534
                     /locus_tag="MJE33_000507"
     CDS             47280..47534
                     /locus_tag="MJE33_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY9646480.1"
                     /translation="MKKKYKHPSYYLISSAMDGDETAIEKLLVFYDPYISKCCLRPLY
                     DEYGNVYIVVDMELKGRIREALIKMILDFEIDIDIETEEE"
     gene            47906..48109
                     /locus_tag="MJE33_000508"
     CDS             47906..48109
                     /locus_tag="MJE33_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBY9646481.1"
                     /translation="MNNTDVPIWEKYTLSIEEASRYFRIGEKKLRRLAEENLDSGWVI
                     VNGNRIQIKRKQFEKIIDTLDEI"
     gene            48187..49377
                     /locus_tag="MJE33_000509"
     CDS             48187..49377
                     /locus_tag="MJE33_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBY9646482.1"
                     /translation="MSKVKRQDSKGRNLRLGESQRKDGRYVYKYTDIFGKPQFVYAWK
                     LVPTDKTPAGKRDDISLREKEKQIKKDLDDGIDTVGGKMTVCQLYEKKNNQRKNIKRN
                     TKIGRQYLMNALENDPLGMRAIDTVKQSDAKEWAIRMNDKGYSYKTIDNYKRSLKASF
                     YMAIQDDCIRKNPFNFKLSDVLEDDTEAKVILTPEQEEKLLAFMETDNVYSKYRDEVI
                     LLLETGLRISELCGLTTHIDMLNRVININHQLLRDTEVGYYISTPKTKNGKRKLPLTE
                     RAYQALERILKNRGKAQPLVVDGYSNFLFLNREGLPKVAGNYESMVRGLIKKYNKTHK
                     DKLPNITPHSFRHTYCTNMANKGMNPNTLQYIMGHANITMTLGYYAHGTFQSAKAELE
                     RLAS"
     gene            49617..50438
                     /locus_tag="MJE33_000510"
     CDS             49617..50438
                     /locus_tag="MJE33_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBY9646483.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKDLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDDISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGMPIEKIEKEVPFAGILLKNNEDIESMQRHL"
     gene            50459..51655
                     /locus_tag="MJE33_000511"
     CDS             50459..51655
                     /locus_tag="MJE33_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBY9646484.1"
                     /translation="MFIEFFYLLRSRGLNVSLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDAVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKSINTEWILKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFKRKYSKIVWMNPELRDGWDSINYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMVSR"
     gene            51823..55221
                     /locus_tag="MJE33_000512"
     CDS             51823..55221
                     /locus_tag="MJE33_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBY9646485.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNILLTYPEIEFIEKPFILQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSDGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELRMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLQNNITEEVEF
                     IVGEGERILNINIWPDFVDDFSVHLVNPSNNQTQAISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWKIVFEPIDIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVITVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIVSFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKGARRLSNQSY
                     PNNSSGFGFLNLSDIDLYTLSNINQDLETEDIGYRSINKSFKDEENSYKFIDGYNMQI
                     HNDLENEIYISKNASRQSGILSGIDIVHTPEFEEELAGLGMSQRFFKISDSLGVLSIN
                     NTDYNSIQRVLQLPSIIRTVSTTKMTLLGEINRGTFGGVVATEEMGVNFFKNNPNINI
                     TGRGTLISIADTGIDYLHPDFIYPDGTSKIVYLWDQTKEGTPPDGFYIGTEYTREDIN
                     RAIAENDPSLSQDEVGQGTMLSGICAGLGNVNSEYAGIAEDSELIIIKLGKIDDFYNS
                     AMLFAASQYAYKKAFELGRPLVINMSLGTSSLAGLTNRSNSEKAFFTRGLCITAGAGN
                     EGNTQTHTSGIIPYVGGSVEVELELNEDEEELSLELWLNRPDKADVIIVSPTGEESKS
                     VGISNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYII
                     TGRYNLYLPNRELLKSGTRFREVDPFYTINYPAIQDDLITIGAYNTINGSLWQSSSRG
                     PTIEDRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYP
                     NQAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            55231..56904
                     /locus_tag="MJE33_000513"
     CDS             55231..56904
                     /locus_tag="MJE33_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBY9646486.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPLDFDET
                     ILNNIIQVAWWEESEPMSSLIEITNNVNNGETITTAAETDYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSELNIAINRND
                     GSLSQDNIGTGTLVSGILVGNGRINSQYRGITTESDLIVVKLKSYTDTYYAGRINYSV
                     SDFLAAITYVTNIARTENKPLIINLTIGVKSSAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEVSHD
                     IRYSPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWILRLTSELI
                     INGEYDIYLPNKNLIAPDTRFLDPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVASGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQDNV
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            57177..57371
                     /locus_tag="MJE33_000514"
     CDS             57177..57371
                     /locus_tag="MJE33_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HBY9646487.1"
                     /translation="MESKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVEEAKDWVDNGSKL"
     misc_binding    57540..57773
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            57850..59247
                     /gene="asnS"
                     /locus_tag="MJE33_000515"
     CDS             57850..59247
                     /gene="asnS"
                     /locus_tag="MJE33_000515"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBY9646488.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFIRITYTKAVELLIESGHEFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     gene            complement(59420..60595)
                     /locus_tag="MJE33_000516"
     CDS             complement(59420..60595)
                     /locus_tag="MJE33_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBY9646489.1"
                     /translation="MNLEKIKKFFGFPLDKKHKDNVEKELCNYLYHTGKIICIIIVIS
                     QIIMMISISAREGGPFVTPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYIN
                     LWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
                     LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEIS
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCNEADAKLKSECLRKII
                     ESCNFERDDVPLKCVTVSIGVYTKKIGKTITLNEFISNCDKALYTAKEQGRNCVAFY"
     gene            61014..62378
                     /locus_tag="MJE33_000517"
     CDS             61014..62378
                     /locus_tag="MJE33_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBY9646490.1"
                     /translation="MGEKKINNMNYSHNNYSKKTGEPTRKIKNSHITLKSKEEKICSS
                     CNKENNTKDNYCKFCGNELYEIASLRPLETKLDLKSKIKELSYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLVNPAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKTVLANSLGVGIFYGLVLAILSIFTNVKTSSH
                     SMLEYGYALEYSYEFFSVLLNGFVLGFICTYITTYKKSYEKENMYLSLFSNSIRTFIL
                     GYVLVLVILLVLTISDSSYLNELDMSSYSNGLNLFTILPQIASYMWAFANGISVTIIN
                     STVSMFTLSSSSLFGDTKLMFYAMGALSMLILLLNGYKLRFKYNTDSIRPIIVFSIYY
                     AFLMGILALFSTFILDSNINFFNTTNYGTTLIMQFKVLQAIVISFVYSFVISLIGYKL
                     NSAD"
     gene            62400..62891
                     /locus_tag="MJE33_000518"
     CDS             62400..62891
                     /locus_tag="MJE33_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBY9646491.1"
                     /translation="MEGNTNNKVDDKFSKVKKKFGSGKFKVELYKEKSKLNREIDDIQ
                     EEKAKIFLEMGILTYQKIREDSINDNSFDKLCDELLELDKLIYEKNMEINEMEMAESN
                     VTCECGYVGNVNDKYCAECGKKFELENECDDFIICGYCESEIDTEAEFCPCCGRKIII
                     DLE"
     gene            63164..63835
                     /locus_tag="MJE33_000519"
     CDS             63164..63835
                     /locus_tag="MJE33_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBY9646492.1"
                     /translation="MLDKVKGRLIVSCQALEDEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCVSTTLSGYTPYTKTLEG
                     PDFVLMERLVKDLEIPVIAEGKVNTPQDLKKVFELGVHSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            63920..64786
                     /locus_tag="MJE33_000520"
     CDS             63920..64786
                     /locus_tag="MJE33_000520"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HBY9646493.1"
                     /translation="MKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGLYVGGS
                     TGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGYDAISA
                     VTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENEKIIGV
                     KFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRARQIFE
                     LTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKEATDEM
                     KSRAKEIYRKYF"
     gene            64819..66321
                     /locus_tag="MJE33_000521"
     CDS             64819..66321
                     /locus_tag="MJE33_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HBY9646494.1"
                     /translation="MVGFTWIDFAILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSIVLAGLTGIPVNVLIIV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFVGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIAVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YRMPEVTSWAYSLITITTSVVVGTIVSAIEAKVKAKVSLPKAETTVYYEK"
     gene            66451..66903
                     /locus_tag="MJE33_000522"
     CDS             66451..66903
                     /locus_tag="MJE33_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBY9646495.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DIFVNIVEYKTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEKGDFL
                     PLDGKNNGYMELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
     gene            67063..67866
                     /locus_tag="MJE33_000523"
     CDS             67063..67866
                     /locus_tag="MJE33_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646496.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESIIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWFLNDTIEKF
                     NTGCTTLSKHYNIENKYVIWIQGESDAKYGSDVLYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACSECDILCVGSKIAMQFHDRGLTIDDVHYTQE
                     GLNMLGEDLCKNIYKYHITKEKSVLSDTIDLSEARKYICELEKISKRFV"
     gene            67924..68811
                     /locus_tag="MJE33_000524"
     CDS             67924..68811
                     /locus_tag="MJE33_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBY9646497.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGFIIDKSSLDTEAKEKGGEGIL
                     AKIKDIVKKYIEENEISGICISTAGMVDPVEGKILFALEELIPNYKGMQLKKEVEKEF
                     NIKCEVENDVNCAGLGEMWLGAGRGATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNVNGSSFQELASTSSLIKKVAKIKNLNENDLNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            68830..69678
                     /locus_tag="MJE33_000525"
     CDS             68830..69678
                     /locus_tag="MJE33_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBY9646498.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
     gene            69741..70430
                     /locus_tag="MJE33_000526"
     CDS             69741..70430
                     /locus_tag="MJE33_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBY9646499.1"
                     /translation="MSKLKDYNIDKVIIFNNLSTKTKNQLKEKVKLKRLQKKEILFYE
                     KDSLDSVYILLEGKVSIFKISENGERKVIFILNSGEVINDITLDASKNSTVGCEAFEN
                     SIVAYYSIEEFLEIMQNDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYR
                     LSREFGVCCGEWTLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELIKIDNKGFYVK
                     ETELSRYFKNN"
     gene            70821..71828
                     /gene="asrA"
                     /locus_tag="MJE33_000527"
     CDS             70821..71828
                     /gene="asrA"
                     /locus_tag="MJE33_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HBY9646500.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNAFEEMEFAKKANFSPKEVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEMIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVISKEE
                     V"
     gene            71829..72620
                     /gene="asrB"
                     /locus_tag="MJE33_000528"
     CDS             71829..72620
                     /gene="asrB"
                     /locus_tag="MJE33_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HBY9646501.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCNTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIVGFKSPKDILFADDLKRWSKKLDI
                     LVTVDGAEEGYSGNVGLVTKYIPELDMKDINNTSIVVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
     gene            72633..73604
                     /gene="asrC"
                     /locus_tag="MJE33_000529"
     CDS             72633..73604
                     /gene="asrC"
                     /locus_tag="MJE33_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HBY9646502.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAVVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            73661..74437
                     /locus_tag="MJE33_000530"
     CDS             73661..74437
                     /locus_tag="MJE33_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBY9646503.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALVLGIIFVGTGLVDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPTI
                     STVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            74708..76726
                     /locus_tag="MJE33_000531"
     CDS             74708..76726
                     /locus_tag="MJE33_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBY9646504.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYVFSYDLK
                     RAVYVPFGLMYIFMVSIPVGKSEFPMRLSALAVGAVIIMIAQFVMNRNRMKNVGDKEL
                     ISICDELLEKISLLKNTINDDSSIIKSSMRKIDSCNYRINSISKNLKMVIFDNRKDDF
                     YISIRGIDIMNILFSLERISLILERYKKDSKEFEDEDIKNILEESSINSKSDVLVVAT
                     KEINYIKMCLENKDTITDKEILGFRDYVISQDTKNINLKEIYSVLENLYEFLVDYKKV
                     KSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHLR
                     DGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLLA
                     GYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFILP
                     YDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNLLLIPSLIEDRLMLINAI
                     YKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKILRDTNYISNYNVDKYDEG
                     RSVILESIVNTKSLGDKIICLNLLQTLNGVKEMYRISNITKVSIKEAA"
     gene            76940..77797
                     /locus_tag="MJE33_000532"
     CDS             76940..77797
                     /locus_tag="MJE33_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBY9646505.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            77894..78763
                     /locus_tag="MJE33_000533"
     CDS             77894..78763
                     /locus_tag="MJE33_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBY9646506.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
     gene            complement(79134..79766)
                     /locus_tag="MJE33_000534"
     CDS             complement(79134..79766)
                     /locus_tag="MJE33_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBY9646507.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
     gene            complement(79838..80191)
                     /locus_tag="MJE33_000535"
     CDS             complement(79838..80191)
                     /locus_tag="MJE33_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
                     /protein_id="HBY9646508.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVYSFGKAQFVYSWK
                     LVPTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLYAKKNVLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            complement(80269..80472)
                     /locus_tag="MJE33_000536"
                     /pseudo
     CDS             complement(80269..80472)
                     /locus_tag="MJE33_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
     gene            80601..80810
                     /locus_tag="MJE33_000537"
     CDS             80601..80810
                     /locus_tag="MJE33_000537"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646509.1"
                     /translation="MVQVTAKQPFQRLFTNHYIVALLIKVMAYSICWLSVSQTYLSTL
                     FSCQRTVGIDLPIKNHNSLWAFIDK"
     gene            complement(80862..81092)
                     /locus_tag="MJE33_000538"
     CDS             complement(80862..81092)
                     /locus_tag="MJE33_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY9646510.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINVILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            complement(81094..81501)
                     /locus_tag="MJE33_000539"
     CDS             complement(81094..81501)
                     /locus_tag="MJE33_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBY9646511.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFLNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMQEH"
     gene            complement(81862..82300)
                     /locus_tag="MJE33_000540"
                     /pseudo
     CDS             complement(81862..82300)
                     /locus_tag="MJE33_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(82314..83240)
                     /locus_tag="MJE33_000541"
     CDS             complement(82314..83240)
                     /locus_tag="MJE33_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646512.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKKERCLISGIGLERFLVAVS"
     gene            complement(83365..84147)
                     /locus_tag="MJE33_000542"
     CDS             complement(83365..84147)
                     /locus_tag="MJE33_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HBY9646513.1"
                     /translation="MTKTQQEMIQNVLEFSKRDSRIIGLAISGSYITKSLDEYSDLDF
                     LIVADDKSYEEVIEERLLIVEQFGTLVSAFTGEHVGEPRLIISLYDSPVLHVDFKFTT
                     LDGLLDRVEDSAILYEENNCITEIYSKKEAYFPTPDLQWIEDRFWIWVHYTCTKIGRR
                     ELFETIDFLSFLRQSVLASLIQLLLGKLPRGVRKIELDAPQYIEALEKTVANHEYKSC
                     VNALQATINLYLELREELSQSPIVRKVEAERVALQYFQDLIK"
     gene            complement(84303..84923)
                     /locus_tag="MJE33_000543"
     CDS             complement(84303..84923)
                     /locus_tag="MJE33_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBY9646514.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            complement(85050..85718)
                     /locus_tag="MJE33_000544"
                     /pseudo
     CDS             complement(85050..85718)
                     /locus_tag="MJE33_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            85810..86625
                     /locus_tag="MJE33_000545"
                     /pseudo
     CDS             85810..86625
                     /locus_tag="MJE33_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            87296..88063
                     /locus_tag="MJE33_000546"
     CDS             87296..88063
                     /locus_tag="MJE33_000546"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBY9646515.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
BASE COUNT        31709 a        12686 c        16866 g        26814 t
ORIGIN      
        1 atttgttaaa aaaaatgcat aatttattgt aatttattga cgaaggttga aaaatattca
       61 taaatgtgcg aaataacaga aaaaatgcaa aaattaattc aatttcattt gttataaaat
      121 ctatatggca aaattaaatc tgcctatcag aatgatataa acacatcaga aaggaggacg
      181 ctatcaaata atacaagtag ttaactttga tagcaaaaaa ttatgaatga acaaactaga
      241 atatccttag agagagctgc tgaattaaaa tcaaaaattg atgattatat tcaggctaga
      301 aaaacgatta acagaggtct tgaaaaagaa gaagagataa ataaacgaaa acagaaaata
      361 ttaagtatct taaatggaac tgaagaggat tggaataact acaaatggca attatcaaat
      421 agaataacag atgtagatac tttatcaaaa attataactc taactaaaaa agaaaaagaa
      481 tatataaaag aggttggtac tcaatttaga tgggcaatat ctccatatta tttgagtctt
      541 atagacccag aagatatatg tgacccaata aaattactgt ctataccaac acatatagag
      601 ttggaagatg agcaagaaga tttggaccca atgggagaag agtatacaaa cccagcagga
      661 tgtataacta gaagataccc ggatagatta ataataaatg taacaaatga gtgtgctatg
      721 tattgtagac actgtcagag aagaagaaat attggacaac aagattctca taagtcaaaa
      781 gctattatcc aagaatctat agactatatc agagaaaatg aagaaataag agatgtacta
      841 gtaactggtg gagatgctct tacattaaaa gatgattatt tagagtggat tcttagccaa
      901 cttaaagaga taccacatgt tgattatgtt agattaggta ctaggactct tgttacaatg
      961 ccacaaagaa ttacagatga attttgcaat atgctaaaaa aatatcaccc tatatatata
     1021 aatactcatt ttaatcatcc aatggaaata actaaggaat ctaaagaagc ttgtgaaaag
     1081 ttagcaaatg caggcgttcc attaggaaat caggcagtat tattaaatgg aataaataat
     1141 gataaatttg taatgagatg tttaaatcaa gaattactga aaataagagt aaaaccttat
     1201 tatatattcc aaagtaaaca tgtaaaggga acaaaacatt tcaatacatc agtagatgat
     1261 ggtcttgaaa tcatggagta tttaagagga tatacatcag gaatggctat accaacatat
     1321 atagtaaatg ctccaaaagg aggaggaaag actcctttgc ttcctcaata ccttgtatca
     1381 aaaggaacag attacgttat gcttagaaca tgggaaggaa aagttataaa aatggaagat
     1441 gaacctgctg tagatataaa gaaacttata aaagaacaag cacaggatta aaaaaatatt
     1501 taaagtttat aaaaagaaac aactttaaaa taaaaagaaa atggatgtca aatttatatc
     1561 aatttgcatc cattttttat tagttattta ttattttgtt gttgattaac taattcaaga
     1621 tatccacttg ggtggaagta atatatctca gaagtatttt catttacaaa gtaaccaata
     1681 tcaccatctg catctggtaa aaatacataa ccattaagat ttttagcttt taatgcttca
     1741 aaatcactag catttccttg atatatgtaa tcaacttcat tgttgtattt tactaaaagt
     1801 tctttagctt gttcttgagt caatttagta ttttggctta caggaacatt cttttgagaa
     1861 tctgcataag aagattcatt tactggtgca gttaataaca aacaagataa aactaataaa
     1921 gatataagtg cttttttcat actaaaaccc tctttcatgt ttaattatat aagattcatt
     1981 acttaataat attgtatgtt tcctataatt aaatattacc attaaagaga ataatattac
     2041 agttacacag ctgtaacttt tgattacaaa atcgtaatta aattgtattt ttattccatt
     2101 tgtattttat catgtctacg atttacaaac tcacagaagt atatctttat aacgcccatc
     2161 acaggagcag atagaatcat accaggtata ccaaagaatc ctccaccaac agtaactgct
     2221 aggattgtaa agaatgggct aagacctagt tgtccaccta cgatttttgg ctctataaat
     2281 gtaacttcaa cttgttgaat tattattaag taaattaaag aagttaaagc aattgttgga
     2341 ttggagaata aattaattat tacaactggt gccataccta ttactggacc aaaatatgga
     2401 accatattca tgacacccat aagtgtacca aataatagtg catattcaga ttttaaaaag
     2461 taaagaccaa ttccagaaag tactccaact ataaaagaat ctaaaatctt acctgtaaag
     2521 tatttaccaa tatttaaatt taagctttga catagttcta ttataaagtc tccatatttt
     2581 ttacctaagg aaatataaac tacttttttt gtaaacagta agaatttttc tttttctaat
     2641 aatatataga aacaaattat aaaacctaaa agaaattgta ctataaattt tccaactgaa
     2701 aatgtagtgc taaatatccc accaagtgaa ccaataagca agttactaaa tttaggcatt
     2761 acactcataa ttttgtcacc atactctttt aatgtattag ggtcaactga ttgtaaactc
     2821 ttgcctaagt cgaataaaaa ctgctctgtt ttatctatgt atgtaggaat ttgagcaact
     2881 aaatcagcta aactatttac tatggatgga actgtaaata atataaacga aattagtata
     2941 actatgagta caccataagt aagaagtaac gaatatattc tcttaaaatt taatttactt
     3001 tctaagaact ttactagtgg gttgaatata tatgctaata caaatgctat tataaatggt
     3061 gttagtagtg acattaataa acttatcact ccaaaaaaat atttgtaatt atcaataact
     3121 ttaatcaaga tatatgaaac aactacagtg agtacaatat ttagataaat ttttttatct
     3181 tgcaaaataa cacaaccttt cttatattta ttaaatttag atatacttaa atctaagtta
     3241 aagttaatat ttatagctta ttatacaata aaaagcagta ttaatatagt atgattaata
     3301 tgaaattata aatacgttaa aactttatgt ttatagctaa aataataaaa attatttata
     3361 ttgtgtttat atataatcta aaatgaaaaa aagtggtgcc tttgattaaa ctaagttttt
     3421 ataatttaaa ttttgtacaa taagctatct tttattattc tttaaaatat atatttcatt
     3481 taattatgac aaataaagat aaaaattaaa tattttagag gtgattttat ttagatatag
     3541 aattttagaa taatactata caaatgtatt gtattattat ttttatatca aaaacacact
     3601 aaaaatttga ttatccgcta aaacgctgta tctatcggca ttatcaagga gctaaacaca
     3661 tcaaaatact actaatttac tacgattgaa tgagccttga tacgtcctat ttcccagata
     3721 tgcaaagata cggtacagca catcagtatt gaaacaagaa aaattgaata cttcatagac
     3781 tacggaacgc acaatatcag cgttcctttt ctatttccag ccgttcctag tggacggcta
     3841 ttttactacc aaaatgaaag gagcatttag aattatgaaa aagatattaa aacgattgtg
     3901 tacaggcttt cttgcctttg caactgtcgt taccgcttta ccgagttcaa cggttcatgc
     3961 gtctaataca cagtattgga cggaaagtaa agagcgtgtc ggtatcgtag aacaagtaat
     4021 gaatgacggt tctatcagtt ctacctttaa tgagggacat ttaaccgttg agggcaagga
     4081 cgcttattgt attgacatca acacagcgtt taaaaacggc tataagacca gaacagacgc
     4141 aagctcacgc atgagtgccg accggataag cgacgttgcc ttatctattg agtatgtgaa
     4201 acagtacaca gacagccaca gcggaataag cagtaaacac gcctacctat taaaacagct
     4261 tgttgtctgg cagagattaa gtgttcagct tgactggcaa tgcgataatg tgcgagcttc
     4321 ctatgatgaa atcccaaaag ccgtacaaga tgaagtattc gcaggagcaa aagcctttgt
     4381 gaaagaaaac aaaggacgtt atgactgtgg cggatatatc tattctggcg agggtcaaga
     4441 gttagggcaa ttctgggcga agttagctgt cggaaatgcc aaacttcaaa agacttccac
     4501 caatgccaat attacagacg gcaacgggat ttattcaatc gctggtgcaa catacggtgt
     4561 gtattctgat aaagactgca cgaaacagct tgccaccctt acgactgata ccagcggaaa
     4621 tacagaagcc gttgaagtaa gagccactac agtatatatc aaggaacttt ctgcaccagc
     4681 aggatttaaa attgataaaa cagtttactc tttatctgtg gaagctggaa aaacagcgac
     4741 tttaaaagta tctgatacac caaaagtaac agataccttg attgagctat tcaagattga
     4801 catggaaaca cagaaatcca attcgcaggg aaacgcttct ttagagggag cagagtttac
     4861 atggaacttc tacgctggat attacaacaa gaacaatctt ccagcacagc ctacaagaac
     4921 atgggtaaca aagactatcg ctgaaaaaga cagcgacgga gctatccatt acattacccg
     4981 tttagctaac gcttataaag tatctggaga cagcttctat atgcaggacg gtaaaaacgt
     5041 gcttccttta ggaacactaa ccgtcgaaga aacaaaaagt ccgtctggtt acttattaga
     5101 gggtgcatat atgcaggcag acggtagcga agaacagata aaagaaatgt atctgacaca
     5161 gattacagag gacggcgacc ttgccgtatt gtctggaagc aatcaatacc atgtttctga
     5221 taaagtcatt cgtggcggtg taaaaatcca gaaacgagat ttagaaacca aagacacgaa
     5281 agctcaaggc ggtgcgactt tgaaaaatac cgcctttgaa atcatttcct taaatgagaa
     5341 tgccgtctta gttgagagca agttatacaa gaaaaatgaa gtgttaaaga caatccatac
     5401 cgacattgag ggtatcgctt ctacttctgc tgacctatta ccttacggaa aatacagatt
     5461 gtctgaacaa aaaccgccag agggatattt gactgacggt gcaaagccga ttgattttga
     5521 aatcacagag aatggaaaaa tcgttgattt gactgacgaa gcccactcta tctataacca
     5581 gattaaacgt ggcgatattg agggcgtaaa aattggtgca ggttcgcaca agcgtcttgc
     5641 agatgttccg tttaggatca caagcaagac gacgggcgaa agtcatgttg ttgtaactga
     5701 tgataacgga caattctcta ctgcttctga ttgggcttcc cacaagcaca ataccaatgc
     5761 aggaaaaaca agcgaggacg gtgtgtggtt tggtacttct gaaccagacg acagcaaagg
     5821 tgctttgatt tatgatacct acatcattga agaattacgt tgtgaaagca acaaaggctt
     5881 caaacttatt ccaccgtttg agattgtcat ttccagaaat aaggttgtgg ttgatttagg
     5941 tacgctgact gatgaatacg aaaaagaaat cactatccat actactgcta ccagcaagga
     6001 cggcgaaaag actatccttg cagggaatga cgttactatc attgatacgg tcaaattaga
     6061 cggtcttgta aaaggtacaa agtatcagtt aaaaggctgg cagatgttga aaaaagaaaa
     6121 tgccgagctt cttgtaggtg ataaacgagt ggaaagtgat tatacctttg tcgctgatga
     6181 tgaagctatg aaagtggaaa tcgcttatac attcaatgcg tctgctcttg gcggtaagaa
     6241 ccttgtaact tttgaagaat tgtatgattt aagcaatcca gaagaacccg tgaaagttgc
     6301 ggaacataaa gatattgagg acgacggaca aacggtactt atcacagaac gtatcatcaa
     6361 aatccatacc acagctacgg acaaggacgg caagaaagag attgaagctg gcaaggacgt
     6421 aacgattgtt gatacggtca agttagaggg cttagaagtc ggtacaaagt atcagcttgt
     6481 gggctggcag atgttaaaag aagaaaatgc agagcttatc atcaatgata aaaaagttga
     6541 aaatgactac acatttacgt ctgacagtga aactatggaa gtaaagattg catttacttt
     6601 tgacgcttct tctcttggcg gtaaacagtt tgtaactttt gaggaattat acgatttaag
     6661 caatccagac aaaccgataa aagttacgga gcataaggac attgaggacg acgggcagac
     6721 ggttactatt aaggaagtac cagaaacacc tacgccagaa gaaccagaga agccaactac
     6781 accagataca ccaacgaaaa cagacagtcc aaaaacgggc gacaatacaa atattcttgc
     6841 ctttgcgatt atgatgtttg tttctgctgg cggtctggct ggtacatact tcttcaaacg
     6901 ccgtaagatg aagaaatcat aaggcggtaa ctgcatgaga aaagaaaaat cacgctctcc
     6961 gccgtaactt aaataggcag actacttcta ttctggcttg tctgccgatt acctgcaaga
     7021 acccagatcg tcaagagtgc gtcagcatac agtttaccct ttactgtctg ggcttgctgg
     7081 tatctcatta actgccagaa tacaaaacaa tcaaactaat agaaagaaac gaggtaaaac
     7141 aatatggaat taaaatatat cgtgccgaat gtggcacaga cttttggaaa cttggaattt
     7201 gcaggggaaa acaaagtaga ccagcaacat atcaatgggc gttacactcc cgtatcaaga
     7261 agttacaact tgtattcgga tattcagaga gccgacgaca ttatcgttgt attgcctgca
     7321 aaagctggcg agaagcattt taagccagag cagaaagtaa aactgattaa cccacgcatt
     7381 accgcagagg gttacaacgt caaaggacgt ggatttacaa actacatttt aatggctgat
     7441 gatatgttac cagcagaaag caaataaggg ggtatttgat tatgagatta gcaaatggaa
     7501 ttgtattaga taacgaaaaa acttttggag tattgaaatt ctctgcactt cgacatgagg
     7561 tgcgtgtaga aaatgaggac ggaacgacaa gtgatgaaat taagcgtcgt acttatgatt
     7621 tgaaatgtag tgtgcaggaa cgcatgatac aagtgtcaat ccccgctgat gttcccgtaa
     7681 aggactttcc ttataatgcg gaagtggagt taatcaatcc cgtagctgat acggtggcaa
     7741 atgccaactt tagaggggct gatgtggact ggtacatcaa ggctgacgat attatcttga
     7801 agaacaaagg caaccagcca gcaggaaatc cacagaacaa caatccacag aaccttgcac
     7861 cacaaggaca acaaaaaaat aaactggcaa actagatttt cattgtagca aatataagtg
     7921 actgttataa tatgggtatg gaaacagata atttgaactt gcggaggtag cgaaaatgaa
     7981 atttaaaaat cctatgttgg tagtgactga tatagataaa tcagttgagt tttataaaaa
     8041 agtttttgga cttcgtgtaa ttatggattt tggagcaaat aaaactttaa caggtggctt
     8101 agccttacag acaagtgaaa catacaaaga gtttatcggt acaagtgata tttcttttgg
     8161 cggtaaaaat tttgaagtct attttgaaga agatgatttt gataaatttg ctgataggct
     8221 gaaagaatat gatattgaat atgtccaccc tattatagaa cattcatggg gacaacgtgt
     8281 agtccgcttt tatgacccag ataaacatat tatcgaagtt ggagaaaaca tgaaaatagt
     8341 atgcaagcgt ttcttaaata gcggaatgac accagaacaa gttgcagaac gcatggacgt
     8401 gcctatgaaa tttataaatg cctgtatgag ataaattctt gtttgtagaa gtcaataaat
     8461 acaaagataa ataagaagtt ataaaggaga aatgagtatg ctggataaaa tacctaatgc
     8521 agaagaaatg actgctttag tcgggcaatc attatatgct atatggaata aactgtgtgc
     8581 cttaattgat gaaaagtatg acatggaatg tttatggaac aaaggtggta aagcgtggac
     8641 ttatgagtat aaatatcgtc gtggcggaaa aacgctttgt gcattgtacg caagggaaaa
     8701 ctgtgtagga tttatgatta tcttagggaa agatgaacga ttaaaatttg atacagatag
     8761 aaatagttat tcaaaagaag tgcaaaaaat ttatgatgaa acaaaaacct atcatgacgg
     8821 aaaatggttg atgtttgagc caacagatac aatcttattt aatgattttg ttaaattatt
     8881 gggaataaaa agaaagccca acaggaaatg atgattttat acttcaattt agtatacaaa
     8941 tcggaagttg aggaggacaa ctattatgga taaagtagtt atttatatac atggtaaagg
     9001 tggaaatgca gaggagtctt cacattacaa acctcttttt agtgattgtg atgtggttgg
     9061 actcaactat actgcacaat atccatggga agcaaaagaa gaatttccat cactttttga
     9121 tttgatttgc aaacattaca aatctgttca aatagttgca aatagcatag gtgcctattt
     9181 cgccatgaac gccttatcag ataagcaaat agaaaaagca tattttattt cacctattgt
     9241 gaatatggaa aaactgattg cagatatgat gtattgggca aatgttacag aagatgaact
     9301 tcgagataaa aaagaaatta aaacaacttt tggagaaaca ctctcatggg aatatctctg
     9361 ttatgtaaaa gaaaatccaa ctacatggaa aattccaaca aatattttat ttggtgagaa
     9421 agataacttg acttcttatg caactatatc tgaatttaca aaacaaacta atgcaacact
     9481 tactgttatg agaaacggag agcattggtt tcacacggaa gaacaaatga aatttcttga
     9541 tgagtggata aacaagtctg ccagataaat cccagtttgt aaaagtcaat aaatacaaag
     9601 ataaatttga agttaggaga ctggtactat gaaaggattt gaacgaaaaa accaattatt
     9661 ttctctttgc ggattgaatt gcggactatg ccctatgctt ttgggaaatt actgtggcgg
     9721 ttgtggcaat ggtaatcaat catgcagaat tgcaaaatgt agtcttgaac atggacagat
     9781 tgaatattgc tatgaatgca aacaatatcc atgcgaaaaa tatcaacaca ttgatgatta
     9841 tgattctttc atcacgcata aacagcgaaa agcagattta gaaagagcaa aaaacatagg
     9901 cattgagcaa tataatcttg aacagcagga aaaaacacaa attctttctt acttactttc
     9961 taactataat gacgggcgta gaaagacttt cttttgtgtg gcagtcaatt tattagaact
    10021 ttcagaatta caagaagcaa tgaaacaaat acaatccaat gacgaattga ccttactacc
    10081 atttaaggca caatgcttgt atgtggtgga ggtgtttcag aagattgctg aaaggagaaa
    10141 tataaaactg aaacttttca agaaaaagtg attttcaaat ttgaaattgt agcaaagagt
    10201 tgtaactgca ttgggaagct atttatatat cctaatgcct atactgaaat tcaaacaaac
    10261 tgcaaaatat catgaggaat atacgctggt attttctaag gaaaacatta aatttaaaac
    10321 acaaagcatt gactcagaag tgaagtggga catttactct gcactatggg aaagccatga
    10381 tttttattac ttaattcaag caccacgatt atatacgtta attcccaaaa gagtttttaa
    10441 agacctaaac gaaaaacagc tatttgaaga aattgcacaa tcaagagtta aaacaacaaa
    10501 gcatatataa tgaacatacc ctacaatgta acaacttcat attcatacac acaaagcggt
    10561 tagtctttta agattgactg cttttttcat acttaaattt aagattggag tgattgattt
    10621 atgcgaataa tttggaataa aggacaccgt atcagagcca gcgataagaa cctcgtctat
    10681 cgcttttcat tgggaacgct tctaattgta ttcgtggtgg ttcttctgct actgaatatg
    10741 aaacagctca tgcgtaccga ctgggaacat ttcagcttat tagacaacag ctttacgctt
    10801 tccttataca acttcataac cttactgata gcgactggtg tttgtgcatt ggtcgctttt
    10861 ctgtattacc gcttttgtta tgacaatttt aaaaagctac tgcaccgtca aaagctggca
    10921 cgaatgatac tggaaaataa gtggtatgaa gccgataccg tacaagacag cggttttttt
    10981 actgacctgc aaagcagatc aagggaaaaa atcgtctggt ttccaaagat ttattatcaa
    11041 atggaaaagg gactgcttca tatccgctgt gaaatcacat taggaaaata tcaagaccaa
    11101 cttttacggt tagaggataa attggaaagt ggcttgtatt gcgagctgac cgacaagacc
    11161 ttacatgacg gctatattaa atatatcctg ctttatgaca tgatagcaaa ccgcattact
    11221 attgatgaag tacgggcaga aaacggctgt cttagactga tgaaaaatct tgtctgggaa
    11281 tatgacgcac tccctcacgc tctgattgct ggtgggactg gtggcggtaa aacctatttt
    11341 cttctgacgc tcattgaagc cctactgcat accaacgctg tcctttacat cttagaccca
    11401 aagaacgctg accttgcaga tttgggaaca gtcatgggaa acgtgtatca cactaaagaa
    11461 gaaatgattg attgcgtcaa tgccttttat gagggcatgg tacaacgaag cgaagaaatg
    11521 aagcaacacc cgaactataa aacaggagaa aactacgcct atcttagctt accgccctgc
    11581 tttttaatct ttgatgaata tgtggcgttt tttgaaatgc tggggacaaa ggaaagcgtg
    11641 agtttactta gccagttaaa gaaaatcgtc atgctgggga gacaagcagg gtatttcctt
    11701 atcgttgcct gccagcgacc agacgcaaaa tatttctcgg acggtatcag agataacttc
    11761 aatttccgtg tagggttggg acgtatcagc gaattaggtt acggtatgct gtttggttct
    11821 gatgtgaaaa aacagttttt ccagaagcgt atcaaagggc gtggctattg tgatgtggga
    11881 acaagcatca taagtgaatt ttacacgcct ttagtcccga aaggatatga ttttttgcag
    11941 accataggtt ctcttgcata tgcaaggcag gacgggacgg cgacgtgcga agcgaaaggc
    12001 gacggcacgg actagccgtt gctggtgtgg ctaacgccac accagcagaa acaagcccct
    12061 cattatctaa cagaggggca caattacggc ggaaacttcc acaaaaacct ttgcaagacc
    12121 gcatggaatg agggtttttc gtacatctcg ccttttgtga taaaaccttg aaattatcac
    12181 aatatcacaa aattatcacg attgggggta tgacaactga atgaaactga ttttttaacc
    12241 gccttgaaag cgaaacgctg tgcatacagc gtatcgcaga caaggcttgc aatcatggca
    12301 aatatcagcc gtgaacattt gagccgtatc gaagcaggaa aggtaacgct gactgaggac
    12361 atgaaacata cgcttttaga agcggtggaa aaattcaatc ccgacaatcc catgttcctg
    12421 ctgtttgatt atgtgcgtat ccgttttccc acaatggata taaagcatat catcaagaat
    12481 atattaaagc tcaatatcga ctatatgctt cacgaagatt acggacatta taaatacacc
    12541 gagcattatc atatcggaga tgtgtttgtg tatgtatcgc aggacgagga aaaaggtgtc
    12601 ctgctagaac tcaaaggcaa aggctgtcgt cagtttgaaa gttatctgct ggcacaagag
    12661 cgaaattggt atgacttctt cacggacgca ttggttgagg gcggtgtgat gaaacgtctt
    12721 gatttggcaa tcaatgaccg aacgggttta cttgatattc cagagctgat acaaaaatgc
    12781 gagaatgagg aatgtatttc taaattccgt tctttcaaaa gctatggttc tggggaactg
    12841 gtaaagcata acgaaacgga taaatacggt atgggacaca cgttgtatat cggttcattt
    12901 tcaagtgaag tatacttctg ctgttatgaa aagaattacg aacagtacgc aaaacttggc
    12961 ataccgattg aagaagtccc cataaaaaat agatttgaga tacgtttgaa aaatgaacgt
    13021 gcctattatg ctgttgtgga actgctgaca cattatgacg cagaacagac agcattttct
    13081 attatcaacc agtatatccg ctttgctgat aaagaaccag acaagcgaaa aagcgactgg
    13141 aaaacaaatg tacgctgggc ttggttcatt ggcgaggggc gaccacccgt aaagctgaca
    13201 acaaagccaa aaccgtacac gttagaacgt acactcaact ggctacaaag gcaggttgca
    13261 ccgaccttga agatgttgaa acagatagac aaggacaatc agacggagta tctggaaacg
    13321 attgagaagc aggcaaaact gaccgagcga caccaccaga taatcaagca acagacagcc
    13381 acggttgaag aaatggttgc caaagtaaag gagtgatgaa aaatgtggga attattgaaa
    13441 gacttcctgc tggtatctct gggaatgggt atcggcgtag tcttgatgtg cattttaact
    13501 gtcggcaaag aagctgacag atgtatggaa gaaatcaaag aaagagagga acattaaatg
    13561 aattttggac aaaatttatg tgcgactcgt tccttagtga aaagctaagg cactaataaa
    13621 agaacgaaaa agtttgatta ttaggagaac tgataatctg acaggtggaa tgatgaagta
    13681 acgctctgaa acgcctgccc tgagcctccg acaagcattg aatgaggaaa ctcagaatgt
    13741 ttgaagctcg gtaaagacgg ctgaaagata ccgtaatagt tgatgaacat atatcttcta
    13801 tcgaaaacta tccagaggtg ggtagcgtat ccaacaggtc gggggtctat aaaatatcta
    13861 tggttagaat gttccacaag ggactgacga aacagcgaat gtacgggtct aaatttgtac
    13921 tttgtagaaa tgcaaaggta cgagaaatcg tagctggtgt ggcaaagtaa gaatataggt
    13981 tatgaaatgc catataccgt tacaggcggt atcaagccag caggcttata gctagtgacc
    14041 taaggatata tatgtataga taagattatc ggaacgagga aaggtattgg gttctcatca
    14101 agagaataag ctgacgaaga acaataagca gttgaaccaa tgctgaaaag cagagggcga
    14161 acctaagaaa ttcttgtaat gagaatggag gaataccctc aagtcgtgta tatattagaa
    14221 aagtattatg ttgttttaat aaggattacg agtaagataa aaaagctcac tcaatttcaa
    14281 gcaaaggagt gaagccttat gcctaaaaat aaaaatgaaa cattgtgtgt agaagattta
    14341 agacacgctg aatattacga aatgcaaaac acctttgatg atttatatgc caaaagtaag
    14401 aatggagata ttttcactca tcttatggat attatcctat cgagagaaaa tatattactg
    14461 gcatatcgaa atatcaaagc aaatgcaggc agtaagacag caggaacaga cggtacaata
    14521 attaaagata ttggcaaatt gccagcagaa acagttgtca aaaaggtaag atatattgtc
    14581 gcaggcactc cacacgggta tcgacctaag ccagtaaggc gaaaagaaat accaaaacca
    14641 aatgggaaaa ccagacctct aggcattcct tgtatgtggg acaggcttat acaacaatgt
    14701 atcaaacaag tattagaacc aatctgcgaa gctaaattta gtgaaaacag ctacggtttc
    14761 agacctaata ggtcggttga aaatgcaata aaagcaacct ataaccgcct acaaatcagc
    14821 cagctacatt atgtgattga atttgatatt aagggtttct ttgacaacgt gaaccattcc
    14881 aaactgataa aacagatatg ggctatggga atacgggaca agcacttgat attcatttta
    14941 aaaaggatat taaaagctcc aattaaaatg cctgacggca caataacata tccagagaaa
    15001 ggaactccac aggggggaat tatatcccca ctactagcca acattgttct taatgagtta
    15061 gaccattggg tagaaagtca atggcatgaa aatcctgtta caaaaaacta tgtggttcat
    15121 atcaataaaa gcggtagccc atgtaaaagc aacgcttaca aagaaatgaa gaaaacaaag
    15181 ttaaaggaaa tgtatatggt tcgttatgca gatgatttta gagttttctg tcgctacaaa
    15241 gaaagtgctg aaaaaaccaa aatagcaatt actcaatgga tcgagcaacg actaaaattg
    15301 gaagtatcac aagaaaaaac aagaattgta aatgttagaa aaagatactc cgactttctt
    15361 ggtttcaaga ttaaaatgat accgagaaga aagaagttag ttgttaaatc acatataagt
    15421 gataaacaat tcaagaccca aaaagacaag ctggtatcac aggcaaagaa aattgcttct
    15481 ccctcaaagg gaaaaacaga attagaggaa ataagacttt ataattcaat ggtattaggt
    15541 atgcaaaatt attatcagat tgcaacaaat gtaaatctgg attgtatgaa acttaataga
    15601 gctgtaatgg ctatcttcac taacagatta ggtgcaagca aaaagggcag attgagaaaa
    15661 acaggcagac aacttacaac aaaagaagca acaagatatg gtaaatctaa aatgcttcga
    15721 tatgttgctg gcgagggcga acctatattc cctgttggat atatccaaca cagaaaacca
    15781 atggcaaaag tgtattcagc aaactgctat actccagagg gaagaaaatt tatccatacc
    15841 aatctaagtg ttgataaatt actgatgtat cagttgatga aattatcttt ggaaaatcgc
    15901 acaatagaat acgcagacaa tcgaatttcg ctattttctg cccaacatgg aaaatgtgct
    15961 ataactctgg aagaatttca acaagctaat gaaatacact gtcatcatat tgtaccaact
    16021 agggctggtg gtaatgacga ttataaaaat cttattttgg taaaagaggc ggtacatcgt
    16081 ctaatacacg caaaaacaga agaaacaata caaaaatata tagctctatt gaaactaaat
    16141 aaagtccagc tcataagact aaataaatat agagtattag ctggcaatca agaattaaac
    16201 ttgatatagg tctactacat aaatactttt caatataaac acgatggaac gcggagtgcg
    16261 gtgaaagtcg cacgctccgt gtgaatgggg ggaaaagttt gagatattta taatatcagg
    16321 aacttaccta tccatataca cttggttttt aagcaacgca cagagtttag tgcttatggc
    16381 gattgtggta attggtatct acttaggttt taagcgtgag ttttcaaaac ttatcggctt
    16441 cttggtagtt gctcttgttg ccgttggctt ggtattcaac gctggtggtg tgaaagacgt
    16501 attgttagaa ctgttcaata agattatcgg tgcataaaat tttattgtag tgtgagtgta
    16561 gaaatggtat gatatggata tgaagaaagc aacaaattag aagttggagg aacaataatg
    16621 gactacaaag tagatgataa aaaccttgat gcatcaatgt ttatttcttt tgtcaatcaa
    16681 gtatggcagg gcgattatga tatggaacaa acaaggcaag cattatctaa aacactaaac
    16741 ataacagcct atgacaataa aaagttagta gggtgtttgc gtattctttc cgatggttac
    16801 tattttggaa caattacaga gctacttgtt cttcctcaat atcaaaaaca gggtattgga
    16861 agtaaacttc ttcaacttgc aaaagacaat acacctacaa tgttatattt cggttctcaa
    16921 ccagaagcag agaagtttta tgaaaagaac ggctgtcaaa aaagcttaca atcttacatg
    16981 ataaataagg gataataaga gataacttcc agcttgacaa ttttatatcc atatacacaa
    17041 agcagttagt cttaggattg actgcttttt tcttacctaa aaattagatt ggagtgatgt
    17101 aatgaacgat atttttaaga atatgcaggc aaaaatcggc tgtgaatatc tttctgacct
    17161 gccctcttac aaacgtaagg tgtggcatga aatgaaacaa ctgaaccttg ccgattatga
    17221 agagaaacag ttagaagatt tttcaaagta tgtgtttggt atgtcatacc agaccttaaa
    17281 agatgtgatg aaacaacaga taggacgtga ggaacaatgc aggaaacaag ggtactggtg
    17341 gaaacgaaaa gaacaactga cgaagaaaca acatcatact ggttcgacct gccgatagag
    17401 gttgccgaat ttgaggaaaa attaggtgtc ggtgcagaaa gtggggaata ccgtattatc
    17461 gaaaaggtat tgccttatgc tgatgaagtc cacgaacata caagcgtgta ccagctcaac
    17521 aaattagatt ttatgtaccg ccagctttca agcgatatgc aggaagaata tgtatcacta
    17581 cttatggtgt ttgagaattt agaagcactt tatatttgca gaaatgtaat taccgtttat
    17641 cccgactgta aaagtatgat agatgttgca aggcaaaagc tgatgaacga cccgacgttc
    17701 aaacatttat ctgaggactg tcaagaatac tatttcaatt ttgaagccta cgcttctcac
    17761 ttgcaggaac acgggaaatt tttagtaaca gaacacggta tctttgaact gccagagtag
    17821 gaagtgaggt ggggcttatg attgatgata tggcggttta tattgctaat cttggcaaat
    17881 acaatgaggg atatttagtc ggtgcgtggt tctctttccc tattgacgag gaagatgtga
    17941 aagaaaaaat cggtttgaat gaacaatatg aggaatacgc tgtccatgat actgacaact
    18001 tccctatcga cataggggaa tatgtttcca ttgaagaact caatgagatg tatgaaatga
    18061 tagaggaact tcccgactat atcgtagagt gtctggacga atttatcagc cactacggga
    18121 cgctggaaga agtcgtggaa cacaaggacg atatttatta ttatccagac tgtgaaacca
    18181 tgacagacgt tgcctactac tacatagacg aattgcaggc acttggcgac attccaccca
    18241 gcttacagaa ctacattgac tatgaagcct acggtcgaga tttggatatg ggcggttgct
    18301 ttattgaaac aagccgaggt atgtgcgaga taccttatta acgctggaag atcagcgaca
    18361 agcattgttg ctactgacag cgtatttctc ttttaaggga cagtctgaaa catggctgtc
    18421 cttttctctt tttaagaact tacgaaagga gctgaaagaa cttgaaaaag attaaaagct
    18481 acacgggtat ctggaacgtg gaaaaagtct tgtatgcaat caatgacttt aacttaccct
    18541 ttcccgttac ctttacacag attacatggt ttgtaattac agaatttatc atcattctgt
    18601 ttggggatat gcccccactt tccatgattg agggagcatt tctcaaatac tttggtattc
    18661 ccgttgctct cacttggttt atgtcgcaga aaacctttga cggaaagaag ccgtacagct
    18721 ttttgaaatc acagataacc tatgccctgc gacctaaaat cacttatgca ggaaaagccg
    18781 taaaactgca taagcagacc ttgaatgaaa caatcacggc agtaaggagt gtgaactatg
    18841 ttcccgataa aatatattga caataacctt gtctggaaca aggacaatga ggtgttcgcc
    18901 tactatgagc tgataccata caattattct ttcctatccg cagagcagaa atttatcgtg
    18961 catgacagct ttcgacagct aatcgcacag tcccgtgagg gtaaaattca tgcgttgcag
    19021 attgccacag aaagctcaat acgaagtatg caggagcagt caaagaaact ggtaacgggg
    19081 aaacttcgtg aggtggcagt ccaaaaaata gacgaacaga ccgaagcgtt agtatctatg
    19141 attggggaca atcaagtgga ctaccgcttt tttcttggct ttaagctcat ggttacggaa
    19201 gaacaactca atctgaagaa tattaaaaaa tcggcatggc tgacgttcac ggaatttctc
    19261 catgaagtga accacacgct gatgaatgat tttgtttcca tgccgaatga tgaaatcaac
    19321 cgttacatga aaatggaaaa gttactggaa aataaaatct cccgtcgctt taaggtgcgt
    19381 cgcttggaaa tcaatgattt tgggtatctc atggaacatc tttacggcag ggacggtatc
    19441 gcctatgaag attatgagta ccagctacca gagaagaaat tgcagaaaga aacgctgata
    19501 aaatactacg accttatccg cccgacaagg tgtgtgattg aggaaagcca gcggtattta
    19561 cgattggaac atgaggacaa ggaaagctat gtgtcttact ttactgtcaa tgcgattgta
    19621 ggcgagcttg attttccgtc gtctgaaatc ttctatttcc agcaacagca gtttacattc
    19681 cccgttgata cttctatgaa tgtagaaatc gtggagaaca gaaaggctct tagcacagta
    19741 agaaataaga aaaaggaatt gaaagacctt gacaatcacg cttatcaagc aggaagtgaa
    19801 accagctcaa atgtggtaga cgctttagac agcgtggacg agctggaaac agacttagac
    19861 cagagcaaag aaagtatgta taagttaagc tacgtgatac gggtgtctgc acccgatctt
    19921 gacgagctga aacgccgttg tgatgaagtc aaggactttt acgacgacct caacgtaaag
    19981 ctggtgcgtc ctgctgggga tatgctgggg cttcattctg aatttttacc tgccagcaag
    20041 cgatatatca atgactatgt gcagtatgta aaatcagact tcttggcagg gcttgggttt
    20101 ggtgctaccc agcagttagg ggaaaatacg ggtatctata tgggctattc cgttgatacg
    20161 ggaagaaatg tgtacctgca accgtccctt gccagtcaag gtgtaaaagg tacagttacc
    20221 aacgcccttg cgtctgcttt tgtcggttcg cttggcggtg gaaagtcgtt ctgcaacaat
    20281 cttcttgtgt attattcggt tctctttggg ggacaagcgg ttatcttaga cccaaaatca
    20341 gagcgtggca actggaaaga aacacttcca gaaatcgcac atgaaatcaa tatcgtaaat
    20401 cttaccagcg acaaggacaa tgcaggactt cttgacccat tcgtgattat gaagaatgta
    20461 aaagacgctg aaagtctggc aatcgatatc ttaacattcc ttactggtat ttcctctagg
    20521 gacggcgaaa aattccccgt acttcgtaaa gcggtacgct ccgttaccca gagcgacagt
    20581 cggggcttgc tccatgtgat agacgagcta cgccgtgaag atacgcccat atcaagaaat
    20641 atcgcagacc atatcgacag cttcacggac tacgactttg cacacctgct gttttcagac
    20701 ggtacggtag aaaatgctat cagtctggat aaccagctca atatcattca agtagccgac
    20761 cttgtgttgc cagataagga cacgaccttt gaggaataca cgaccattga attgttgtct
    20821 gtgtctatgc tgattgtgat tagtaccttt gcccttgatt ttatccattc ggacagaagc
    20881 atttttaaga ttgtagattt ggacgaagca tgggcgttct taaatgtggc acaaggcgaa
    20941 accttatcaa ataagctggt tcgtgctgga cgagctatgc aggcaggcgt ctattttgtt
    21001 acccagtctg cctatgatgt ttcaaaggaa agtctgaaaa ataatatcgg cttgaaattt
    21061 gcctttagaa gtaccgatat aaacgagatt aaacagacct tagaattttt cggtatcgac
    21121 aaagaggacg aaaacaacca gaaaaggctt cgtgatttgg agaacggaca atgcttgttg
    21181 caggacttat acgggcgtgt cggcgtagta cagatacacc cagtctttga agaactgcta
    21241 cacgcctttg ataccagacc gcccgtacag agaaatgagg tggagtgatg aaagaaagga
    21301 tacagagtgc gttcacaaaa aagaagatgt tccgctttct caagataacg gtgtttgtcg
    21361 tggcactttc ccttatcctg ctttcactac ttgaaacggt ggctcatgca acggggcttg
    21421 tggacgatac gataaataca gaaaatttat attctaaata tccgttatcc aactatcagc
    21481 ttgattttta tgtggataat agctgggctt ggttgccgtg gaactggctg gacggtatcg
    21541 gaaaatcggt acagtacggg ctttactgta ttactaactt cgtttggact atcagtttat
    21601 atttaagcaa tgccacgggc tatgtggtgc aggaagccta taagctggac ttcatcaatg
    21661 atatggcaga cagtatcgga cagagcatac agacccttgc aggcgttacc tcaagcggtt
    21721 tttcaacctc tggcttctat gtcggtttcc tgcttctcat tatcttggtg gtgggaattt
    21781 atgtagcata tacgggactg attaaaagag aaaccagcaa ggcacttcac gccgttatca
    21841 actttgttgt ggtgtttatt ttgtcggctt catttattgc ctatgctccc gactacatca
    21901 aaaagataaa tgaattttca agcgacatca gtaccgcttc tttggacttg ggaacaaaaa
    21961 tcatgctccc aaactctgac agcgagggca aggacagcgt ggacttgata agggacagcc
    22021 tattctctat tcaagtggaa cagccgtggc tactcttaca atttgggaac agcaacgcag
    22081 aggaaatcgg gacagaccgt gtggaagctc tggtatcggt aagcccagag gacgaggacg
    22141 gaaaaaccag agaggaaatc gtgaaaacag aaatcgagga taacgacaac aacattctga
    22201 cgataccgca ggtagtaaac cgtttaggta tggtgttttt cctcttattc ttcaacttag
    22261 gtatcacgat atttgtattc ttgcttacgg gcatgatgtt gttcagccag atacttttta
    22321 ttatctttgc aatgttcctg cctattagtt tcctgctctc tatgataccg agctatgaaa
    22381 gcatggcaaa gcaggcaatc gtgagggtat ttaataccat aatgacacgg gcaggaataa
    22441 cgctcattgt aacggtggcg ttcagcattt ccagtatgtt ttacaatatc tccacagact
    22501 acccgttctt tatggtggcg ttcttgcaga tagtttgttt tgcaggtatc tatatgaagc
    22561 tgggcgattt aatgagtatg ttctcactta atgccagcga cagtcaaagc atgggacgca
    22621 ggatattccg cagaccgtat ctgtttatac gacatagagc gagaagaatg gagcgacgta
    22681 tggcaagggc ggtaagtgct ggcggtattt cgggcggtgt cgctggtgcg ctggctggca
    22741 gtaccgtttc taatgcaagg acaacaagaa acaatcctac aaaggaaaat cggggcaata
    22801 ccacttccat catgggacag cgtgcaggct caaagatagg cgctgtctta gatacgaaaa
    22861 ataaagtgaa agacaaggca aatgctgtca aagagaatgt taaggatatg ccgacacaga
    22921 ccgcttatgc ggtgtattct gcaaaagaaa aggcaaagtc cagcgtgtcc gacttcaagc
    22981 gtggcatggt gcaggaacag caatccagac agacgggacg cttagaaaag caggaacaac
    23041 gtaaaaaaaa tatcgctaac aagcgtatgg agcttcaaaa ggcacaagaa gcaaggcagg
    23101 cacgaaaggc tgacggatca gcgacaacgg gagctacccg tccccatgag cgaccagcca
    23161 cagcttcaaa gccgagtgtg gaaaaagtgc aggaagtcaa acgccctgcc acaactccga
    23221 cttcaaaagc aagtgagcca gtcaagacaa atgttatcaa agagcgtccg ctatcttcgg
    23281 gtgcttctga cggaaaagca acccagtcgg cacagccaat acataggcag aatatagaaa
    23341 aagtggtatc gcaggaaaca cgccagaatt acgttaaaga ccgcaggaca aaggttcagc
    23401 agacgcagag cgtccagaag aaccagcaga ccacagagaa aaaccataac cttgtaacga
    23461 agaaaggaca gaagaaaaaa tgaaactgaa acatatcgct cttatcggca gtctgtttcc
    23521 tatcctcttt tccatagtcc ttttttttgg ggtgctgatt agtgcggaca gcgacgacga
    23581 aaacagcaac ttttcttctg gtattacggg tttgaaccta tccgcagaag tcttgaaaca
    23641 tcagcctatg gtggaaaagt acgccagaga atacggcatt tccgagtatg ttaatgtatt
    23701 actggctatc attcaagtag aaagtggcgg tacagcggaa gatgtcatgc agtccagcga
    23761 aagtctggga ctaccgccca actctttaga tacggaaagc tcaatcaagc aggggtgtaa
    23821 atattttgcg tccctgcttt cttcctacaa aaatcaagga ataaatgact tgaatgtggc
    23881 gatacagtcc tataactatg gcggtggcta tgtgggatat gtggcaggaa aagggaaaaa
    23941 gcatacgttt actcttgcag agagcttcgc ctgtgaaaaa tcgggtggaa agaaagtaac
    24001 ctacaccaac acgatagccg ttgcgaagaa tgggggctgg cggtatggct atggaaatat
    24061 gttttatgtg gaattggtca atcaatatct gactgtaccg caggtatcgg gagaactggc
    24121 acaaaagata atgaatgaag cgttgaagta tcaaggttgg aagtatgtct atggtggcag
    24181 taatccgaac acttcctttg actgttcggg acttgtgcaa tggtgttatg gaaaagcggg
    24241 tatctcttta ccgagaacgg cacaagccca gtatgacgca acccagcata tccccttgtc
    24301 gcaggcaaag gctggcgatt tggtgttttt ccattcaacc tataacgctg gttcgtatgt
    24361 aacccatgtc ggtatctatg tcgggaataa tcagatgtac catgcaggcg acccgatagg
    24421 atatgcagac ctcaatagta gctactggca acagcactta atcggtgcag gacgagtaaa
    24481 ataaaagaaa ggacttagaa aatatgttta agaaaagtaa gaaacagaca gaaaatgtca
    24541 aagaaaaaaa ggtgcgtact gtcaaggtag gcacacataa gaaaactgta attgcgtttt
    24601 gggtggtgct tatcgtaagt gtaagttttg gggtatataa gaattttacc gccattgata
    24661 tgcacaccgt acatgaaaca gaaaccatac aattaagact taccgatact aacgggattg
    24721 aaaatttcgt gaagaacttt gcgaagtcct attacacatg gaacaacagc aaagaagcga
    24781 ttgaagcaag gacgcaggca atcagcggtt atctgacaaa ggaattgcag gacttgaatg
    24841 ttgataccat tagaaccgat ataccgacca gttccacggt tacagatgtg attgtttgga
    24901 atatcgaaca gtcaggaacg gacacttttt ctgctaccta cgaagtagat cagcagataa
    24961 aagagggaga acagacaaca gcagtcaagg caatctatac ggtaaaagtc catgtggacg
    25021 ttgacgggga tatggtaatc atacagaacc ctacgcttgc accaacaatc gaaaaatcgg
    25081 actatgagct aaagacacca gaagcagacg caagtgtgga cgctgatacc ataaatgacg
    25141 ctacttcatt tttggaaaca ttctttaagc tgtacccaat agcgacggaa aaagagcttg
    25201 cctattatgt atcgggaaat gtgcttgaac ctatcggcaa ggactatctt tattctgaat
    25261 tgataaaccc agtctttaca aaagacgggg acaatgtgaa agtcaaagtt gccgtgaaat
    25321 tccttgataa tcaaacaaag gcgacgcagg tatcgcagta tgaacttgtg ctacataagg
    25381 atagtaactg gaaaattgtg aaataataat ttaaagcgtg catttcttat aagatttgta
    25441 cgctttactt ttatggtaaa aattagcctt gacaagcgac cgttggtcgc tatataatga
    25501 aagcgaaaca agaaacgacc gatggtcgct ataaggaggt atcataaata tgccaaaagg
    25561 aattatgctt acacaagaac aacaagaaga acgccgagaa gaaataatca gtgttgcttt
    25621 gcagctcata gagaaaaatg gattccaaaa aacatcaatg aggaaaattg cgattttagc
    25681 aaacatggga aagtccagtc tgtatgattt tttcaaaacg aaagacgaga tttttgtata
    25741 tgcagttgag aaagaaatag aagaaacaat taaaaaggtt cataggatta ttgccgatga
    25801 atcctcgccg gaacagtgtc ttagaaaaat catgctgaat catcttggag taccaaagca
    25861 gtatcgaacg gtgctgatgt ggttaaatac agaatctgat tatttagaag aagattatcg
    25921 aaagcggctg aaaactgcgc gttacgcata tcaggatatt ataaaatctg taattgaaaa
    25981 tggaatggaa tcaggcgtct tccgtaagac gaatgccaat cttatgacgc gtttgttaat
    26041 taactccatt atatcaatca tttacacatc ccgaccatca gccagtccgg agaaaatgct
    26101 tgatgaaaca atggatatat ttctacatgg aattatgaat gatggaggtg aattattatg
    26161 atttattata ctctaccctt gttgcataaa caggcaaccc ttgaaatggt cggcggaaaa
    26221 ggtatgtctt tatcaaaact tttgacaacg ggtattcctg tgccggaggg ctttcatgtt
    26281 acgactactt catacaagat ttttgttgaa aagaaccata ttcagcctta cattaataag
    26341 ttgctggacg gtattgacac taacaatacg agccagcttg aaaatgtatc tacgcagata
    26401 gggatgcttt tccataatgg agaaatgccg caggaagtat cagatgcaat taaaaccgca
    26461 tatgccagat tgggaaatat agcggtggct gtccgttcct ccgcaaccgc cgaggatttg
    26521 cccgatgctt cttttgcagg ccagcaggaa acctatctta acatacaagg tgagaatgaa
    26581 gttcttgacg ctgtaaagag gtgctgggct tccctatgga cagctcgcgc tattgcttac
    26641 cgcgtgaaga atgatataaa gcaggaaatc gtcgcacttg ctgttgtagt acaaaagctt
    26701 gcgttttccg atgcgtccgg cgttatgttt acgctaaacc ctatcaatgg cagacgtagc
    26761 gaaatgattg ttaacgccgc gtggggactt ggcgaatcgg tggtttctag cttagtcacc
    26821 cctgatacaa tcgttgtaga taaaaatgct gaacgaattg tatcgtatga agtagcaaac
    26881 aaagagatta tgacagtacg caactcagat ggaacagaag aaatcccaac tcctgaacag
    26941 ttgaggaaaa aacatgctct tactcgcaat caagttatgc gattgacaca gcttggaaaa
    27001 aaaattgaga agtattatga aatgcctatg gatgtagagt gggcactgga aaaagataag
    27061 ttgtatattg ttcaggctcg ccccattact gtcctaccgc cggaatggac gttaccagaa
    27121 aaggatgtta tatatacaag aggcagtcta gcggaacact tgccaaatcc tgttacgccc
    27181 ttattcgcca cacttgggct tgaaatagtc aaccgtgcgt cggcgctttt atggatagat
    27241 atgtttggta aaagtgcgaa aaagctactg ccagaaaacg gagcatatac gattattaac
    27301 ggatatgttt atctttccgc taattcaaag cctcttttga ttgctgtcaa atccctttca
    27361 ccccgatctt tacgccgtgc gctcacgaac agtgttgcac gctgggaaac agcacgaaaa
    27421 gaatttgagg atgtgattaa acaatgggaa gaaaagccca tgcatatgct gaatgctcat
    27481 caaataatgg aggggattca gactgtattt tatggagcgt gtatttattt cacgaggatt
    27541 cagcttacat tgccagccgc ctctatcagc gaaacattat ttacaaaatt ttttcagggg
    27601 gcggctcgtc gcgctggtat gacagatact tctgttttcc tgcttggatt tgatacgatt
    27661 gctctgcaag ccgaaaagaa cctgtggagt ctctcggaat gggtaaagca aaataatact
    27721 ctcaaccttt atctgcaaag taatccaaca gcaaagatag cggaaaattt tatgtcctca
    27781 gttccgcctg acgaagtttc actggaagta tggatagaat ggaaaaatcg aatcaatcag
    27841 tattttaaag agtttggtcg taccgcttat gaatttgatt ttgcatattc cacaccgcag
    27901 gaaacactta caccaacctt tgaatcgata aaaacctttg tagaaggcaa aggtgaaagt
    27961 ccttttttac gtcaatccaa atttgaaaag cataggaaac aggcagaaga agaaatttta
    28021 caacatatag gtagctcacg caaaaaactg tttttgaagt tgctccattg ggcacaggaa
    28081 actgctccta tgcgtgaaaa tgctatttat tgcatgggaa tggggcatcc tctgattcgc
    28141 cagatgtttc atgaaatttc agcacgcctt atacgtggcg gagctacttc gcatatagat
    28201 gatatttatt ggctcacaaa attggaattg gaaaaactca tagtacagtt agacaaaaat
    28261 atacctttat ccgatagaag aaattcgata cttgcgcgaa aagcggaact caaaaagtat
    28321 cagggctata tgccaccagc tcagttacct gagaagaaag taaaaagcat atcacatgca
    28381 ccacagaaac aaaaagatgg aaaaaccgtg ttgaggggta taggaaccag ttctggtgtc
    28441 gtgacagctc gtgcctgcgt actaaatagt ccggcagatt ttaaaaattt ccagccagga
    28501 agtgttttgg ttgccgttac tacaactccg gcttggacac ccttatttgc ttcagcaagt
    28561 ggaattgtaa cggatattgg aggtcctctc agtcattcaa gtattgttgc acgggaatgt
    28621 ggtatccctg ccgttatggc gacacacaca gccacgagaa gtattaaaaa tggacaaatg
    28681 ataacggtgg acggctcgga ggggacggtg acgattgatg aataaaaagc cgattttcgc
    28741 tttaaatctt gctactttgg tgattgcgtt aggatatagc ttaattcttc cggtcatgcc
    28801 attttacatg gaaaatctag gtgctggcgg tcgtgagctt ggatggctca ctgccgtata
    28861 tgccctggca caaacagtat gcgcaccttt ttggggtgca ctgtccgacc gaattggtag
    28921 aaagccgatt atcagcattg gtattatagg atattccatc agtttgttcc tgttcggttt
    28981 ggcttcatcc ttttggacac tatttatagc tcgcagttta tcgggtattt tgtcatctgc
    29041 tacgtccgtt gcatctttgg catatgttgg agattccagc tcggaggaag aaagaagcaa
    29101 gaatatgagt caacttggag cttcaatgag cgttggcgta atgatgggac cattattggg
    29161 aggcattctt gcaggctatt cgcttgcgct cccatttttt acaggagctg gcatttcatt
    29221 tattgcgttt ctgctaattc taacactttt gccagagtca attgagcgtt caggacagac
    29281 ggaaaaaaga aaaccctttg atttttcaga cctgaaaagt ataatttttg gctcagcagg
    29341 aatggttttg atacttattt tcgtcgtgca tttctgtcaa acaggcttgc aaggaattac
    29401 ggggctgtat gtcgtagata agtttggatt ttccactaag caagttggtg ttatttggat
    29461 ggtgctagca ggtattttga ttgtagtaca aggctttttg actggtcctt tagcaaaaaa
    29521 aattggtgag aagcgactaa tactgatagg tatgttttgc aaagccttag tagcgtttgc
    29581 catggtattg acaactgggt ttgttagtgt gttggtggtt tttggcttat ttgcggtatc
    29641 ttctgctatt acagtaccca cattaaacgc aaccctttca aaaactgaca atcagcataa
    29701 aggaacactg atgggatttg ccagtaccgc cggaaatctt agcaaagtta ttgctccttt
    29761 attaactggt tatctgtatg aaagcaatat tgaattacca tatataacca cgggagggat
    29821 agccttaatc ggagtggtta tctgctatat ctggataaaa aatcaaaaga acaaatgatg
    29881 gaagaggacg tttatatgga ggttatacac agtaactaat ctggtataga aattcaattg
    29941 aggcttttct gctttattaa agtcttgcta ctacttaaac ctataatatc cttaattcat
    30001 tatgacaaat tccaattcaa cagttttgat acacataagg aggtaacaat actatggaaa
    30061 attatcaatg aagtgctaat tctttatcag tgaagttatc cataagtttt tctacctgta
    30121 atcgtctggt gctttttgcc agatcattgg taggtaagaa aaattcatca acagacacat
    30181 gaagtaatga tacaaggtca cttgtatgct tggatgttgc cctttatttt caatattagt
    30241 taagtaacgt gggtcaatat ttgtaattcg attttaggga aatatttaaa gaaatatttt
    30301 cgttttattt ctttaaatct tcaagaatat tgatagtttt tttgaaagta ggtgtgtatt
    30361 atgaagaatg agaaaaaaat tacagtcgca ttgctcattg tttatttatt gattttatca
    30421 tggattattt tatttaagct acaattttca ttttctgtgt tagaccacat aagacaaata
    30481 aacttaattc cttttggtgg ttcagttatt gcaaatggaa aaattgactt cgatgaaatt
    30541 ataaacaatg ccattgtttt tatccctgta ggtgcatatt tcagtttgct ttttaaaaat
    30601 aaatctgttc taaaagcaat aggttctgtt ttaggaatta gctttgtata tgagataatt
    30661 caatttatat ttgctatcgg agcaagtgat attacagact taattagcaa cacattaggg
    30721 ggtataattg gaattgcatt agtatatgta ttatctattg ttctaaagga taaaacacat
    30781 aagatattaa atcgaatagc aatagtatgt actgtattag tagttgcctt cttatttatg
    30841 cttcttgctg taaataatat attttaatgc catgttaatt acatgttgtt ttggaaaaga
    30901 ctttaatctg tcaaagtttg cttttgaaat tgaacatgaa attacaccaa tggaacaaat
    30961 gaaagctata aatttttcat tggagaaaga tgactgtaca agtaaagtat taatatacaa
    31021 tccagacaaa tggaaatatg tagaatacta tttctataaa gatagaccta ttaaaaaact
    31081 attggaaaaa tttattcaat tttgcatttg tcacaataaa atatgttttg aaatattgaa
    31141 tacttaaata tgcaaaaaaa acaaatgtaa ctggctatgg ggctatatct actaaaaagc
    31201 tatgtgatag gttgggatta aatttaaaca tcacttctag aaggcatgaa tacacaaggc
    31261 tgacaaacta tacagttatg attaaacgat accaacggga ttgaaaattt cgtgaagaat
    31321 tttgagaagt cctattacac atgagcaaca gcaacgaagc tattgaagca aggacgcagg
    31381 caatctagcg gttatctgac aaaggagttg taggacttga atgtagatac tatcagaacg
    31441 gatataccga ccagctctgc ggttacagat gtgattgtgt ggaatatcga gcagtcggga
    31501 acggacactt tatctgctac ctacgaagta gatcagcaga taaaagagag agaacagaca
    31561 acagcggtca aggcaaccta taccgtaaaa gtccatgtgg acgctgacgg ggatatggta
    31621 atcgttcaga accttactct tgcaccagca atcgaaaaat cagactatga gccaaagaca
    31681 tcagaaacag acgcaagcgt ggacgctgat accgttaatg acgctaccgc atttctggaa
    31741 acattctttg aggtgtaccc gacagccaca gaaaaagaac ttgcctatta tgtatcggaa
    31801 aatgtgattg aacctatcgg cagggactac ctttattctg aattggtaaa cccagtcttt
    31861 acaaaggacg gggacaatgt gaaagtcaag gtagctgtga aattccttga taatcagaca
    31921 aaggcaacgc aggtgtcgca gtatgatctt gtactccata aggatagcaa ctggaagatt
    31981 ttaggataac atggaaatgg gcttgtatca taattatagt gtgcaagccc ttttcatatt
    32041 gaagtatcaa taatcaataa ttataattag gtacatgaat ggagggataa aatgttcaaa
    32101 attggtgagt tttcaaaatt gacacaagtt tctactcgta tgttgcgtta ttatgatgaa
    32161 acgggtttat taaaacctgc taaaattgat tcattaactg gatatcgtat gtattcagca
    32221 gaacaaatct caactttaaa tagaattata tatttacgtg atagtggctt caatgtcgct
    32281 gaaattgcat tagccctcaa tttcagtaat aatgccatta tagaacagct tgatagaaag
    32341 tctattgaaa ttatggataa tatacaaaaa gaacaagaaa aactaaagaa aattgagatt
    32401 gctaaaaaag aagtgttaca tggcaaaagt gaattgtatt ttaacataac cattaaagag
    32461 ataccgagtt attgcgtttt atctttaaga aaagaaatac caaattatta ttatgagggc
    32521 gaactctgga aagaactctc tgtttttgct aaagaaaata aaataaatat ttctgatgaa
    32581 acattctcaa tatatcacaa tgaagaatat aaagaaaaag atgttgatgt agaattatgt
    32641 gccattgtaa ataaaatagg aaaagctaca ggtgatttta agtttcgttt taccgaacca
    32701 gtttctatga tggcttgtac tatggtatat ggagattttt ttaatatttc ttatgcttat
    32761 cgttcatttg ctaaatggct tcaagaaaac agtcaatata agatgaaagg ttcaagccgt
    32821 caaatagttc atcgtggacc gtggaacgag aaaaatccag agaactatct aacagaaatc
    32881 caaattcctt tagaaaatct cttgtaaaaa atgataatct gccttgactt taacatggtg
    32941 ttaaggttta tcgtttattt atcaagaaga aaaaggagga aaatataatg ataacttata
    33001 aagtgaattc aataggtact atctataata atgaaaaagg tgctttcatt caacttgaag
    33061 ctgcatatat cccagcatta agggcattag acggattcag tcatataaat gttttatggt
    33121 ggttcagtga ttttgataat acggatttta gaagtgtatt ggaaacagaa cagccttata
    33181 aaaatgctcc agagaaaatg gggatttttg caacaaggtc gccagtacgt ccaaatccga
    33241 ttgctttatc agtagtggaa attatcaata tcgattatat gaatggaatg atacaaattg
    33301 cttacattga tgctaatgac aaaacgccta tcttggatat taaaccatac acaccgagtt
    33361 tggatagggt ggaaaatcca aatgttccag attggtgtag tcattggcct catagtttag
    33421 aagaatccgc ttcttttgca tgggaaaacg aatttaattt ttaaggagtg atgaaccatg
    33481 agtttgcagg attacattat tgaaaaacca gtaattgata cagaacacct tattttaaga
    33541 acaatggata aaaatgacat agatgatttg aaagaatgga ttagtgatat ttctttatac
    33601 gagtattggg ggaaacgtcc ttcaaaaagc gaaaaaaatc cagaagtact ctttagtaag
    33661 ccagaaaaag ctactaaaag ttttcattgg ggaattgtcc ataaacaaga tagcaaagtg
    33721 attggtgaaa tatggatata ccttattgaa aataatagaa tggcaaaagt ggctttcaga
    33781 ctttctaagc aatatcaagg taatggacta atgagtgaag cgttgaaaag cgtaattgaa
    33841 ttttgttttt caaaaacaga attacaaaaa atatggacag atgtgcatat tttaaatcat
    33901 gcttcttaca agacattgga gaaagtcggt tttcaaaggg aaggacatat tcgaggggga
    33961 aaaatggtaa atacctattg cgactactac ctatatggaa ttttaaaatc tgattatatc
    34021 actagctgat gttatggcta atattgaagc gacgattggc gaagcaatga acaatacagt
    34081 tccaaaggtc tatgatacga aaagcattaa aagatatgtg atgaatttat tagatatggc
    34141 tcattaaaaa gtaccaaaga aataaaagtt accatataga caaaattggt tttttatatc
    34201 ttttgatttc ttataagata atggcattgt gcgacaagct atacaaaaaa ttttcatgta
    34261 tactttgatt ataaattata caaggagcta actatatata aatcaagtga tttttgataa
    34321 ttagctgaaa tattttttag atcattgaaa agtgaatttt gtgtacactg aaaactactg
    34381 ctttatgcag tactttaaaa atattcaatt gaatgctttt aaataagcag tttttcatca
    34441 aattgtatgt tttcagacaa cagtttatag attactctta tgagttttcg tacggaatgc
    34501 ccctgagcac atctatggga tttaccttgt aagcgtttta gtttataata cttattgaaa
    34561 gtgagattgt atttgcatac tgtaagaatg cattgatata gactttttct taggtagcgc
    34621 gaaccacgtt tagacaatgc tgttcgaggt gcactgtatt caccggactg ataagtaccg
    34681 gggtctacac ctgcaaatcc catgactttg acagcagagc tgaagttatg aatatcacca
    34741 atctcgctta aaatcgacat tcccgtataa atcccaatac cgggaatcga gaagatagga
    34801 gaatctagtt tgacagctaa ttcttctatt tttttgtcta cagttttaag ctgactggct
    34861 aacagttcga tattttcaat aagcattttg atttcaaggg taacagcttc gttgttttcg
    34921 ccgatagatt tctttgcact ggaacgaaga gatgatgcat ctatattcac acggcttcca
    34981 ctgcttttag gcttgagaac ttttttaaga tgagcaagat tggcactggc taaattataa
    35041 gaacttccaa attcttttaa gacagccata taagctttgg aatatttagt tttaaaccaa
    35101 gtattgaact caggaataac gatatcaata catttctgaa gttggttttt cttctggttg
    35161 atatcttcag caatagaagc atgataacgg gttaattcac gagcctgttt catatcgagc
    35221 ttacgctgag tgactctagt ataatctcta tcaaagagta ctgaacaaat aagaaaagta
    35281 tcttttttat cattcttagt ctttcttatt ttgcttttgc gcttggcatc agttgttaat
    35341 ggatttatca aagcaacttc ataatcatga gtgattaaaa aattaataag attatcaccg
    35401 taatgaccgg tagcttctaa accgacaata tgtctaggtg atttaaattt tttaacagct
    35461 ttgataaaag aatcgaatcc gtctagatta ttagtgaaga aaaaaggttc aataaggact
    35521 tcaccttcag aagaaataac gcaggcacag tgattgaatt taccaacgtc tataccgatg
    35581 taaaacataa aaagcacatc ctttctttat ataagatagt gatatgtgat taaaccacaa
    35641 acagagtatc aaagagcctg gtgcaaaata caaattcgaa gacttatcta actgatcaca
    35701 attaagatac aaagatgtgg caataacctc cttcaagtag tcaagctaca ggtgctgaaa
    35761 gaaaaagtac acattcacaa ttcaattcta aacaaaattt aatgaaagga aaaggtgtat
    35821 ataattgaca gatagataag tcaattacaa tataccaggt actgatatgt acgcatatga
    35881 aaatattgaa atgtcattaa actatattga acaacattta agtgagaaaa tcgaaacaga
    35941 gaaactggcg gaaattgcct gtctttctac attttattac caacgcttgt ttaaaaagtt
    36001 agttaagaaa tcagttcaag agtatatcaa acttcgccgt ttagcaatgg taatagcaga
    36061 attgaataat tcagaggaaa gaatattaga catagcttta aaatatggat tttcagacca
    36121 tgctaacttc acaagagcat ttaaagaagt ttaccatatt actcccgatg aatacagaaa
    36181 gaatgttcct atgttaaata catttgacaa gccagaactt tcttcacgtt atatcatgat
    36241 tgatgaaaat gtcccattga ttgtaggaaa tattgttttg gaaattcaaa gaaaaacatt
    36301 aagaacaaag gaaacatatt ttggttttga aaaaacagta aatattgctg aacaaattcc
    36361 tgctggagaa agtacaggaa ttgatgttcc aggtcagcta tggcgcgaat tccatgcaaa
    36421 gaaactgcaa accgaaaatg ccatttttaa caatgatatt gaacttggtg tatctcactt
    36481 agcaagtcca gataaaggaa cattccttta ctttgcagga ggtatggttt catcaacaga
    36541 tgtatgtcct aaaaatatgg tacaacatac attaccagca ggtgagtata ttgtatgcaa
    36601 aattgaggca gaatcttttg aagaacttgt tactactgct ttaaatcaag caaataagta
    36661 tctgtttgaa acttggcttc caaatcataa tttggttaca aagccattca tggcagaaaa
    36721 atattacaaa ggaaatgcag agattaatta tatggaagta tggataattc ctgtgtaaac
    36781 agtaaatgct aaataggaat tatctgacgt aataaaaagg aaatagaaag cttataaagg
    36841 agggcatagt ccgttgtgat aaacataaaa gatataaatt atgtacctaa tattagtgaa
    36901 ataagcgttt atattgaaaa ctctgcattt gatgagttgt atcagcatat gaacaatgaa
    36961 tataaagcag tttgcaaaat cgaatacagt aaagatgtct gggcacgagg ttggaacgta
    37021 aagtttaaaa aatctgggaa atcattgtgt gttgtctatc cgaagaagaa ctactttact
    37081 gtattggtgg ttgtaggtaa taaagagaaa gaagatgttg aaaacttgtt acctaggctt
    37141 tctgaatatg tacaggaaat ttatcgtaac actagagagg gaaatggaca aaggtggctt
    37201 atgatagact taaaatctaa aaataatgta tatcaagatg tattaaggct tattgaaatt
    37261 cgttatgcca taaaatgtgt aaaaccgtaa taaaaatatt tttcacatac gtaatctcaa
    37321 ggtattatcg ttgagacaag taacctatat aattatggga aatgagattt ttagatactt
    37381 aagaaagtac tttagggggc ttgcgttatg gaaagattta agtacaattc cacacaatca
    37441 aatacaatct aataaagctt tactaaaaat tgaatgcatt acagctttaa aggctcgtac
    37501 aaactatatg ttttgtgcgg gcttttttca tttaatagga atttggattc gtcagagagt
    37561 atattataaa aataaaacga taagggtacc ttaaaaataa ggtacctcag gctgtagaca
    37621 aagtcccgga ctctcgtctg ggcttttctt atatttgagt tatccacaat tcaacttttt
    37681 aagagttatc ttattaagaa aaaagtgtat gttttccacc cgatagtttt acttctgtga
    37741 tatttgcaga tgccactcaa gtagaaagtg gtggtacggc agaagatgtc atgcagagtt
    37801 cagaaagtct gggactaccg cctaattcct tagacacgga aagctcaatc aagcaggggt
    37861 gtaagtattt tgcgtccctg ctttcttcct gcaaaaatca aggtatcgac gatttgaatg
    37921 tagcgataca atcttacaac tatggcgggg gttatgtggg ctatgtggca ggaaaaggga
    37981 aaaagcacac ttttaatctt gcagagagct tcgcccgtga gaaatcgggt ggaaagaaag
    38041 taacctacgc caacccgata gccgttgcaa agaacggggg ctggcggtat ggctatggaa
    38101 atatgtttta tgtggaatta gtcaatcagt atttaactgt tccgcaggtg tcgggggaac
    38161 tggcacaaaa ggtaatgaat gaagcgttga agtatcaagg gtggaaatat gtgtttgggg
    38221 gaagcaatcc caacacttcc tttgattgta gcggacttgt gcaatggtgc tatggaaaag
    38281 ctggtatcta cttaccgaga acggcacaaa cacagtatga cgcaacccag catatccctt
    38341 tatcgcaggc aaaagctggg gagttggtct ttttccattc aacctataac gctggttcgt
    38401 atgtaacgca cgtcggtatc tatgtgggaa ataatcagat gtaccatgca ggcaatcagc
    38461 ggttatctaa caaagaaatt gcaggacttg aatgttgata ctattagaac cgatatactg
    38521 actagcccca cagttacaga tgtaattgtg tggaatatcg aacagtcggg aacggacact
    38581 ttttctgcta cctacgaagt agatcagcag ataaaagagg gagaacagac aacaacagtc
    38641 aaggcaacct atacggtaaa agtccatgta gacgcttaca gggatatggt aattattcag
    38701 aaccctaccc ttgcaccagc aatcgaaaaa tcggactatg agccaaagac accagaagca
    38761 gacgcaagtg tagacgctga taccgtgaat gacgctaccg catttctgga aacattcttt
    38821 aagctgtacc cgacagccac agcaaaagag cttgcctatt atgtatttgg aaatgtgctt
    38881 gaacctatcg gcagggacta cctttattca gaattggtaa acccagtact tacaaaggac
    38941 ggggataata tgaaagtcaa ggtagctgta aaattccttg ataatcagac aaaggcgacg
    39001 caggtattgc agtatgagct tattctacat aaggatagta actggaaaat tattatgtaa
    39061 gacattttgt tggcaggatc tattttcgga tatagtctgc cttttctgta gcaaatgtac
    39121 tgcactgaca agctatttca ttgtatttta agcaattaaa tctttcttga caattttatc
    39181 ttaaatcata tatttatgga tattgacttt ctgcttgcaa taagttataa tatgactata
    39241 aaaccaaatt agtcacatag gaggtatgtg gtatgtataa agaagttggt atttcttcaa
    39301 ataatgttcc gataacactt tctatctggt atacagaaag aagtagtcct accattgttt
    39361 ttttgccggg tactatgtca caccctctca tgtatgagaa ttttctatgt ggtttgagtg
    39421 aaagaggatt taatataata ggcattcact atctttcaca cggaaaaagc cctaaaatca
    39481 aaaaaaatta tacaatggaa gatttgctgc ataatgtata tgacgcaaca acttacggaa
    39541 tagaaaattt cggtgaaaac atagctgtca tgggttccag tcaaggcggg attttagctg
    39601 caatggcggc aggtagggac acaaggttaa aagccgtttt tccacataat atcatgctga
    39661 ctacattaaa agaaactatg agcttaacaa aatatcccac atggtcgcac cgttttatgg
    39721 ggctgataca gtggggcttt cgtgtaggtg gaaaattgct gcctaactac aaggttccag
    39781 gagacgctta tttagactat gacagagtat tttatagtga acaggcaaaa caagattgct
    39841 taaatgaccc tatgctttta ccctattatc ctttaagatt tgtcgccagc ttatttaatg
    39901 ctgacttatc gtgcctggag aatggggcta tacaatgtcc tgttatttta attactgcca
    39961 aaggcgaccc gctttttagc tttgattata ccaacaaagt atttgattta attcatgtac
    40021 cgcaaaagga attacttgct ttagagctta atcgtcacat gatttttaat gaggacacgg
    40081 acactacaat aaacgcattg gaagctacct tgcataaata tcttggatag catattgatt
    40141 atataatcag taaatgttat acttttcatg acgatataaa tattttagtc ataaggaggt
    40201 tatgagtttg ccacgattta cggaacaaga aaaagaaatc ataaacagta aacttttgat
    40261 agagggagaa aagctgtttg cattacacgg cttaaaaaaa gtaacagttg atgatttagt
    40321 agctgctgta aacatatcaa aaggttcttt ctatgctttt tatcccagta aagaacatct
    40381 atatgttgaa atcaattttc gcttacaaaa agagttattt acaagtatag aaacaaccat
    40441 taaaaagaaa aagtataaga accatagaga tttagcaaag gacgttatca tgctaagttt
    40501 aacgggggtg attacttcgc ctattctttc acagattgat ttatctttaa tggactatct
    40561 gcaaagaaaa atatcgtcag atatttttga aaatcatatg tacagtgata ttcgtatatt
    40621 ggagatattg gaagatttag gggttgtatt tttggttccg catacggtta ttataaaatc
    40681 cctgtattct gttttatcct gtttggaaca attcaaagag gacgaagaac tgaatatgat
    40741 acaaaatctt ttagtgaatg gtattattca gcaagtagta gcagagtaac aattttgttt
    40801 ggttatttga caggcaggta gccaaacaaa ttttttacag tttatatgac tatatatcta
    40861 taagcgtcat aaagttaaag gaggaatgaa taatgattga tgtaaaaaaa ctgtatttta
    40921 gctatactga caaaccgttt gtggagaatg taagtttcca tgttgggaga ggagaaattt
    40981 ttggtttttt aggaccctct ggtgctggaa aatcaactat tcaaaaagtt ttaactgggc
    41041 ttaatacaag atataaaggc agcgtaaaag ttgcaggtac agaaataagg gagcatacaa
    41101 atcagtttta tgaaaatatt ggggtagact ttgaattttc aacctgctac gagaaattca
    41161 cagcacgaca aaatctcgcc tattttgctt ctctatatga aaagcaacct cggtctattg
    41221 atgaattatt gcatatggta ggcttggaaa atgacgggga caaaaaagtt gctgactttt
    41281 ccaaaggtat gcgttctcgc ttgaatttca ttaaagcgtt agtccatgac cctgatatat
    41341 tattccttga tgaacctaca agcggtcttg accctacaaa tagtcgttta atgaaagata
    41401 ttattcttgc agaaaagaaa cgcggaaaaa cgattatcat tactactcac aatatgtttg
    41461 acgcaacaga gctatgtgac caagtagcct ttatcgttgc cggaaaagtc agtgcattgg
    41521 atagcccgca taatttgatt atgtcaaggg gagctgccaa aatacaatat acctactatg
    41581 ataaaggcga gaaaaccgga gaatgtctgc ttgatagaac cacagaagat aagttgttaa
    41641 aaagtttaat ttccgaaaac cgtttgcttt ctattcatag tagtgagcct acattaaatg
    41701 atatttttgt tgatattaca gggaggacac ttcaatgaga ttgggacgtt taatttgtgg
    41761 tgatatacat ttccaatgga aatatggttt ctattttatc tactttatat taacagtttt
    41821 atatgtgtgc gggattgccg ctttgccgga acattggaaa acggatattg cttctattat
    41881 gatctactct gacccggcag caatgggatt attttttatg ggagcgattg tcctcttaga
    41941 aaaaagtcaa aaggtgttaa atgctatggt agtatcgcct gtaaaagtat cggagtacat
    42001 actttcaaaa acaatcgcgt tgattgctat ttctacggta atcgcattga tattaggggt
    42061 agtttccgac agcaatcatt tacttggtat cacagttggt acggctttga cctcagcaat
    42121 ctttactatg cttggaatta ttgcagctac aaaaatttca aacttgaacc aatttctcat
    42181 tgtgattatg ccgatagaaa ttgtttgctt tgtaccacct attgttggtc tgtttgtgaa
    42241 gttaccatat ctgttccgct tttttccatt cacagcttgc atgaacctta taactggaaa
    42301 aagcgtttta ctatcgtttg atatggtact tgtaattgct acgttgatta tcctctacat
    42361 agttgcgcga catacagtta agcatatgtg gaaaagttta ggaggtgtaa agctatgaaa
    42421 aggatattat ccagtaccat acaggtattt agacaaatca agagtgaccc tatgatgttt
    42481 gcagcttgtt ttacgccttt tgtcatggga gctttgatta aattaggtat tccctttctt
    42541 gaaagaataa cggatttttc tttgcaagca tactatccga tttttgattt actactttct
    42601 atcatggctc ctgtattact ctgctttgca tttgcaatga ttacattaga ggaaatcgac
    42661 gataaagtgt cacggtactt ttcgattacc cctcttggta agtcggggta tctctttaca
    42721 aggttgggag taccctcaat tatttcggcg gtcatcgctt ttattgtact gttacttttt
    42781 tcattagaaa agttgtccat tggaatgaca atatgtttgg cgcttctcgg ctcagtacag
    42841 gcaattattg tttcacttat gattatcaca ttatcaagca ataaattaga gggtatggca
    42901 gttacaaaac tagctgcact tacattgctc ggaataccag cccccttttt tattgatagt
    42961 tactaccagt tcgcggttgg cttccttcca tcattttggg tagcgaaagc catgcagaat
    43021 gaggcggttc tttatttctc tataggattg gtggtagctt tagtctggta ctacttcctt
    43081 acaaaacgtc tgtttcggaa gctggcagga taaggaggca tattatggaa aagggtagac
    43141 ttctgcaaaa cggaaaagta aatagagcta ttcaggtttc caaaatcaca ctatttatca
    43201 acctaagttt atcgttactt aaatttactg ccggatatat aggaaagtca agtgccatgc
    43261 tgtcggacgc tgtacactcg gcttctgatg tattaagcac aatcgttgtg atggttggaa
    43321 ttaaaatatc agaaaaacag cctgatagag aacatcctta tgggcatgaa cgtatggaat
    43381 gtgctgcttc cattattctc tctgttgcat tggccataac aggtgctgga attggatatt
    43441 caggtatcaa gaaaatcttt tcagaacagt acaatacgct ttctactcct agtggaatcg
    43501 cacttacggc ggcagtctta tccattgtta taaaagagtg gatgtactgg tttacaagaa
    43561 atgctgcaaa gcatacaaat tctgacgcac ttatggctga cgcatggcac caccgctctg
    43621 atgcactctc gtcggttggt tccttaattg gcattttagg agcgcgttta ggctatgcta
    43681 ttctcgaccc tattgcaagc gttgtgattt gcggttgtat cttaaaagct gctttggaca
    43741 ttttcaaaga aagcattaac aaaatggtag accatagttg tgacaatgct actgaaacaa
    43801 agattaggga agttgtgctt cagcagcaag gggttgatgg tattgatgaa ttaaaaaccc
    43861 gcatgtttgg agcgaaaatg tatgtagata ttgaaatatt agctgatggt aatttagctc
    43921 tttatgacgc tcatagaatt gctgagggag tccatcaaac gattgaaaac aactttccac
    43981 aatgtaaaca ttgtatggta catgttaata cgaatgaact tctctattct gcataggaca
    44041 gggaagatga tttaaggcta ttgaaaggga aaggagggaa gtaggttatg gaacaatact
    44101 ggtggattat cattgttgtg gttttgattg tcgttggttt acttaaaatt gtaattatga
    44161 agaagtataa ccaaaggaga aattctaaca atagtccaaa agatagcgag gacgacaaga
    44221 tgtattaagg tttattgaaa tctgttatgt tgtagaatga tgtttttaga tactagaaag
    44281 tgctttaagg gggctaacgt tatggaaagc tttgagtaca attctttcca atcaacagta
    44341 ttttgcatag agaaatgtat gtaggaaata tggtgcaagg cataaagcac cagcgatcga
    44401 gagatcaacc tatgaaccaa aggcacacta cattttatac tacttgacaa gtaaacgagc
    44461 gtttactata atggtttaca gaagtaaacg ttcgtttact aaagaaggag gttgtaaatg
    44521 tctgacacaa aagaaaatat attaaaaatt gcactacact tatttgcagt gaatggttat
    44581 gaggctgttt ctgttagtat gattgcggaa gccttgggaa tgacaaaagg agcattatat
    44641 aaacattata aaaataagca agatattttt gactgtatcg tagaacgtat gtatcaaatt
    44701 gataataagc gagctaagga ttatcaagta ccagaaagca caatagaggt aacccctaat
    44761 gcatacagaa atacaactat tggaagtcta ataacattta ctcttgcaca atacgatttt
    44821 tgggtgactg atgaatttgc gtctaatttt cgcaaaatgt taatactaga gcaatataga
    44881 aatttaaaag tgaatatgct ttatcaaaaa tgtcttgtaa atggtcctat cagttatata
    44941 gaagatatat ttaatgaaat gattgaaaaa ggtgttcttc aagagtgtga tacaaaacag
    45001 cttgctattg aatattatgc gccattattt ctattgataa gtgtagcaga cgcagttgaa
    45061 aattgttggg atttaaaaac tcaattaact acacacgtta aacaatttgt caagaaatat
    45121 attataaatg agaaagtaaa ataatccttt attctgtcct aaatataaac ctaacttttt
    45181 aatttcagca gagaaactca aaatgaagat tgttataagt cacaaatatt aactattaaa
    45241 atattacaat aacggagatg agaaaatgga atatattcgc agtaaaaaat attttacacc
    45301 cgacttacag acaaaaatta tggggccgaa tcctataaaa ttacaagagg aactattaca
    45361 aaatcataaa atcccgtctg gtagctatgt ctgtgacctt ggaagtggac aaggtttaac
    45421 ttctgtattt cttgcaaaag aatatggatt tgttgttttt gcagttgact tatggagtga
    45481 gccaaaagaa aatgaagaat ttttcaaaaa aatgggatta tcttctgcac aaattaaatc
    45541 tgtaaaagct gacgccaatg aacttaaatt tgagaaagag ttttttgatg ctgtagtaag
    45601 tacagattca tataattatt ttggacgtga ccctatatat ttagatgaaa aattgcttcc
    45661 atttgttaag aaaggtgggt ttgtttatat cacagttcct ggcatgaaga aagattgcca
    45721 tgagaaactt ccagaagagt tattgttatc atggacacca gaagaacttg attatataca
    45781 tgatataaca tattggtcta acataattag tcattgtaaa aaggcagatt taatttcaat
    45841 atatgaaatg gaaagtaatt cggaagtttg gtcggattgg ataaagcaaa ataatgaata
    45901 tgcaataaat gaccgtaagg caatagaagc tggcggtgga gattatctga attttattgc
    45961 tataattcta caaaaaaaat aaattcttta gaaccaaaca ctataatttg cgttctaata
    46021 tgtttaccga ttgacatcaa taattttatt tttgagggta atgatttgaa aatagaagaa
    46081 taattccagt ttgtaaaagt caataaatcc aaagataaat ttgtatttga aaggagtttt
    46141 gaaaaatgaa aaaatatgag tatgtagaaa ttaactataa tgcgaaagga gttgtattcc
    46201 tttgtactga taaacacaaa gaaattattg acagttacgc aaaaacgggg tatcactacg
    46261 cagggtttgt acccacagaa attgacgcaa aaggctgtat gcgaaagata gatttaattt
    46321 ttgaaaaaga agattaactt attttaggga gttggttcta atgataggac ttaaaagacg
    46381 aaatatgaat tatattatga aagttggtgc taaagctcaa ggtgtttctg tatctgaatt
    46441 tagggctatg ctacaagata ccatagacaa aactatggaa agtacagacc cagacgtgca
    46501 ggcaaatttt aaacgatact ttggaaataa gcgcccaaca ccagaggaat atatttatac
    46561 gattaccaaa aagacaaagg ttaaggttta aatgttgtaa agggggcttg ccgtatgaaa
    46621 cgctttatgt acaattcagt atattcaagc ccaatttaac agcatactat ttacaattaa
    46681 atacgctata ccagtagctc gtacaaaatt tatattttgt gcgggctttt ttaatacccg
    46741 aaaagttttt ttaattattt tcattttttt ggaagcaaat cacaccttgc tgtacagata
    46801 tggtgcggaa agagggataa acacttttca attcatagct ggaaaggagg tgagattatg
    46861 aaaccgtctg acttccaaaa aacaattcag tgtcagtttg aatgtaagct gaaaaaagtt
    46921 gtcaaaggta ttatctggaa ctaccgtaaa gaattaaaac gaagaaaaaa caaggaaata
    46981 tcttttagcg aattaccaga cattacggta gaaagtctgg caacatggga cgactacgaa
    47041 acaaactaca caatttttaa tgtgtgtggc atagatatta aagtccttga tgatgaactt
    47101 gcagaagctc tcaaacagct acccgaaaaa aaacgtaaca atgtattgat gtattacttc
    47161 ttggaaatga gtgatacaga gattgcagat attatgaatt ataccagagg ggctgtgttc
    47221 aaacacagac accgtgccct agaaactatg aaagagatac taaaggagaa gaaatagaaa
    47281 tgaagaaaaa gtacaagcac ccgtcttatt atttgatttc atcagctatg gacggggatg
    47341 aaactgcgat tgagaaatta ctggtattct atgacccgta catatcaaag tgttgtttac
    47401 gtcctttata tgatgaatac ggcaatgttt atattgttgt tgatatggaa ttaaagggac
    47461 gtattcgaga agccttaatc aaaatgattt tagactttga gattgatata gatatagaaa
    47521 ccgaagaaga ataagcagat accagcaggg cgtgattggt taaacctcct ctttcctgcc
    47581 ctgctcctgc ttttatacat ggaagcctac ggctttcgcc gattgttctt tgacaactga
    47641 ataaagcaga cagatacgtt tgtgatacag cgagccacgg ggactgtacg ccatgacctt
    47701 tccgacaaga aagtgagcga cccacgcagt gatccgagag caacttgtgg aatggtcgct
    47761 ttgccatgac ctgctatcac agaataatga tacgtccgca tggtgcggtt ctgcccacaa
    47821 gaatgggaat agttgagata ctaacggagc tttccgaagc cgtctgtctg cttatgataa
    47881 gacatacaga aaggagtgtt gcaacatgaa caatactgat gtgcctatct gggaaaaata
    47941 tacccttagt atagaagaag cgtcccgata ttttcgtatc ggggaaaaga agttgcgtag
    48001 gcttgccgag gaaaatctgg actctggctg ggttatcgtc aatggaaacc gtattcagat
    48061 aaaacgaaaa cagtttgaaa aaattattga tacactggac gaaatctgat gtcttagagc
    48121 cgtagatatg gtataatatt agtagcgtat cacggctcat tccatgacgg aaaggagctt
    48181 tacaccatgt caaaagtgaa aagacaagac agtaaaggac gcaatttgcg tcttggagag
    48241 agccagagaa aagacggaag atacgtttat aaatataccg atatattcgg gaagccacag
    48301 tttgtatatg cttggaaact tgtacccaca gacaaaacac ctgctggaaa acgtgatgat
    48361 atatcgttga gggaaaaaga aaagcagata aaaaaagacc ttgacgacgg tatcgacaca
    48421 gtgggtggta aaatgacagt ctgccagctt tacgaaaaga agaacaatca aagaaagaac
    48481 atcaagagaa acacaaaaat aggacgacag tatcttatga acgcactgga aaatgaccca
    48541 ttgggtatga gggcgattga tacggtcaag cagtccgacg ctaaagaatg ggctatcaga
    48601 atgaatgaca aaggttattc ctacaaaacg atagataact ataagcgttc cttaaaggct
    48661 tcattctata tggcgataca agacgactgt atcagaaaaa atcctttcaa tttcaagttg
    48721 agtgatgttc tggaagatga tacggaagca aaggttatcc taacaccaga gcaggaagaa
    48781 aaactccttg cctttatgga aacagataat gtctacagca agtatcgtga tgaagttatt
    48841 cttctgctgg aaacgggact tcgtatctct gaattatgcg gactgacaac acacattgat
    48901 atgcttaacc gtgtgattaa cataaaccac cagctattgc gtgatacaga ggtgggttac
    48961 tatatttcca cacctaagac aaaaaacggg aagcgtaagc ttccattgac agaaagagca
    49021 tatcaggcac ttgaacgaat tttaaagaac cgaggaaaag cacaaccgct tgttgttgac
    49081 ggttacagca acttcttgtt cttgaaccgt gagggattgc caaaggtggc aggaaattat
    49141 gaaagtatgg tgcgtgggct gattaagaag tacaacaaga cgcataagga caagttgcca
    49201 aacatcacgc cccattcatt cagacatact tactgtacga acatggcaaa taagggaatg
    49261 aaccctaaca ccctgcaata catcatgggg cacgcaaaca taaccatgac attagggtat
    49321 tatgctcacg gtactttcca gtctgcaaag gctgaattag aaaggctggc ttcttaatat
    49381 cgaaaatgta tcttactact tatttactac ttttggttgc attttcatgc tggtaaatgc
    49441 catcttatgc gaggtatctt ccaaaaggaa aatgccaata aagcccgaaa atatgggata
    49501 tatcggcatt taccaactta tgaaaagata atcagaaatt tagtaagttt ttatatcaaa
    49561 aatagtaata aagtctatat acatttagaa aatactgttt aaaaggagta ggatttatga
    49621 taaaaaaaga gatatctaac tttagaggaa gtagtgatta tgtagtatca ccagaattga
    49681 tggcatcagt taatgttgct attgctcttg aaaagccact tttgattaag ggcgaacctg
    49741 gaacaggtaa aactatgcta gcacaagcaa tttcaaatga attaaaaaaa gatttagtaa
    49801 tttggaatat aaaatcaact acaaaagcac aagaagggct ttatgtatat gatactgttc
    49861 aaagacttta tgatagccaa tttggaggag aaggtgtaga tgatattagc aagtacataa
    49921 aatatggtaa actaggagag gcctttagct caaatcaaca agtaattctt ttaatagatg
    49981 aaatagataa ggcggattta gaatttccaa atgacttgct ttgggaattg gataaaatgg
    50041 agttttacat aaatgagaca aaagaaactg taagagctaa acaaagacca atagttatta
    50101 taacttcaaa tgcggaaaaa gaacttccag atgctttttt aagaagatgc atatttcatt
    50161 atatagaatt tcctgataga gacatgatgg aagaaatagt taaagtacat tttgataaag
    50221 tagaagaaca cctattggaa caagtaatga ctacttttta ttggattaga agtttaaagg
    50281 acatacaaaa aaagcctagt acatcagagt taatagactg gatacaagca ttaactttga
    50341 gtggaatgcc tattgaaaaa atagaaaaag aagttccttt tgcaggaata ctacttaaga
    50401 ataatgagga tattgaatct atgcaaagac acttatagtt aataggagct ggataattat
    50461 gtttattgaa tttttttatt tattgaggtc aagaggatta aatgtatctt taaatgaatg
    50521 gatgacttta attgaagcat tggataaagg actgtgctat tcaaactttt caaattttta
    50581 ctatctttgc aggatgattt taataaaaag tgaaagtgat tttgataagt ttgatgcagt
    50641 gtttttggag tattttaaag gtatagaaca ccaagaagaa attccagaag agattatgaa
    50701 ttggcttaaa aaaccagata tagatttaga agagtttgaa agattagaac aaaatccaaa
    50761 tataaatctt gataaattaa gagctaaatt ggaggaacga ataaaagaac aagactctcc
    50821 tcataatggt ggtaattatt ggataggcac aggaggcact tcagagttag gacattcagg
    50881 aaaagggcaa actggtatac gtataggtgg aaattctact tatggtagag ctgttcttga
    50941 agttgcagga gaaagaaaat acagagattt tagagatgat gaagttttaa atatgcgtca
    51001 atttcaagtt gcactaaaga gacttcgtca attttctacc agaatagatg ctccaaagac
    51061 agagcttgat ttggataaga ctattgaaga gacttgtaat aatgcaggat atttaaagtt
    51121 attttttgag aagccaagaa aaaatacagt gaagctttta ttgcttatgg attcaggtgg
    51181 ttcaatgaga gggtatagca ctttatgtaa tacattattt cagtctgtaa gtaaatcaaa
    51241 tcattttaaa gatgtaaaga tatattattt ccataactgt atttatgata gattatttac
    51301 aacacctgaa tgttggttaa gtaaatctat taatactgaa tggatattaa aaaatataga
    51361 taaaaactat aaagttataa ttgttggaga tgcttctatg tcgccttctg agttgcttca
    51421 tattggtggt aactatcgtg gtccatataa ttacacacct ggtatagagt ggcttaaaag
    51481 gtttaagaga aagtacagta aaattgtatg gatgaatcca gaacttagag atggatggga
    51541 ttcaattaat tattggtatc aaactcaaag aatgatacaa agtgaattta atatgtttcc
    51601 attgactgta aaaggtcttg agaaagcatt gaaaaattta atggtttcaa gataaagtaa
    51661 atataaatga tggtatgatt tattcaaaat tgtaccatca ttatttattt tttttaatat
    51721 atctattata aattgtataa ctgaaactta aaaaattata tagtctaata cattttagac
    51781 tcacacaaaa catatacatt aataataatt ttagaagtcg aggtgattat aataaattat
    51841 gaattaattg taaaatacaa tggagatata ttgaggttag aagaagaatt aggtgtatca
    51901 gtagagatac ttaattcatc atatgcaata ataacatcaa gtaatgaaga agatgtaaat
    51961 atacttttaa cttatccaga aattgaattt atagaaaagc cttttatatt acagactcag
    52021 gatgtgcaaa gtttttcaag tacaggaata acaggattta aaaatagaac tggtttaact
    52081 ggaaaaggaa ctataattgg tataattgat tcgggaatag attatactct acctgtgttt
    52141 agagatagtg atggaaggtc taagatatta tattattggg accaatctat tcaaggaaat
    52201 ccaccagaag gttttagaga aggaacatta tatactaatg aagatataaa taatgcaata
    52261 gatggaagta tgtatatacc tatttcaaca actagcttac atgggacaca tgttgcaggt
    52321 atttgtgcaa ctattgcaag tgatgccaga ataatagtgg ttagagttgg aaatatacag
    52381 accgatattt tttcgagaag tacagagttt atgagggcaa ttaaatttat tttagatagg
    52441 gcattagaat taagaatgcc tgttacatta aatataagtt atggaagtaa tgaaggttca
    52501 catagaggaa catctttatt tgaacaatat atagatgata tgtgtttatt ttggaaaaac
    52561 aatatagttg tagcagctgg aaataatgca gataaaggag gtcataaaag gattaggctt
    52621 caaaataata ttacagaaga agttgagttt atagttgggg aaggagagcg tatattaaac
    52681 ataaatatat ggccagattt tgttgatgat tttagtgtac atttggtaaa cccatcaaat
    52741 aatcaaacac aagcaatctc tctaacttca ggtgagataa ggaatacttt gggtgaaact
    52801 agaataactg gttattttta ccctatagca ccgtattctc tcacaagaag agttactcta
    52861 caattaagtt caaatacaca gataactcct gggttatgga aaattgtatt tgaaccaata
    52921 gatatagtaa ctggtaatgt aaatatatat ttgccaactt ctgaaggtct gaatagaaat
    52981 actagatttc tgattcctac tcaagagctt actgtaacag ttcctggaac agcaagtaga
    53041 gttataacag taggtagttt taattcaaga acagatatag tatctatatt ttctggtgaa
    53101 ggagatacac aattaggtgt ttttaaacct gatttattag ctccaggtga agatatagta
    53161 tcttttttac caggaggtac aagtggagca ttaactggaa ctagcatggc tacacctcat
    53221 gttacagggg tttgttcatt gtttatggaa tggggaattg taaatggaaa tgatttattt
    53281 ttgtattcac agaaattaag ggcattactt ctaaagggag caaggagatt aagtaatcaa
    53341 tcttatccaa ataattcatc aggatttggt tttttaaatt tatcagatat agatttgtac
    53401 actctatcta atataaatca agatttagaa acagaggata ttggatatag aagtattaat
    53461 aagtcattta aagatgagga aaatagttat aagtttatag atggttataa tatgcaaata
    53521 cataatgact tagagaatga aatttatata agtaaaaatg caagtagaca atcaggaata
    53581 ctatcaggaa tagatatagt ccatactcct gaatttgaag aagaactagc tgggttaggt
    53641 atgagtcaaa ggttttttaa gataagtgat tcattaggtg tactatctat aaataataca
    53701 gactataata gcatacaacg agttttgcaa cttccttcaa tcataagaac tgtatcgact
    53761 acaaagatga cattacttgg agaaattaat cgaggtactt ttggaggtgt agttgcaaca
    53821 gaagaaatgg gcgtaaattt ctttaaaaat aatcctaata ttaatataac aggaagaggg
    53881 actctaatat caattgcaga tacaggaata gactatctac acccagattt tatttatcca
    53941 gatggcactt caaaaatagt atatttatgg gaccaaacaa aagaaggaac tcctcctgat
    54001 ggtttttata ttgggacaga atatacacga gaagatataa atcgagctat cgcagaaaat
    54061 gaccctagtc tatctcagga tgaagttggt caaggaacta tgttgagtgg gatatgtgca
    54121 gggcttggaa atgtaaatag tgaatatgca ggtatagcag aagattctga gctaataata
    54181 ataaaactcg gcaagataga tgatttttat aatagtgcaa tgttgtttgc tgcatctcag
    54241 tatgcatata aaaaagcttt tgaacttgga agaccattag ttataaatat gtctttaggt
    54301 accagtagcc tagcaggttt aaccaatagg agtaatagtg aaaaagcgtt ctttactaga
    54361 gggttatgta taacggcagg agcaggaaat gagggcaata cacagacaca tacatctgga
    54421 ataataccat atgttggagg ttctgttgaa gttgaattag agttaaatga agacgaggaa
    54481 gaattatcat tagaactttg gttaaataga ccagataaag ctgatgtaat aatagtatct
    54541 cccactggtg aagaaagtaa aagtgttgga atttcaaatt ataataaagt aacaggttta
    54601 tttgatttag aggggactga atattctata acatatatat acccaacaac tttttctgga
    54661 cagcaattta ctaatgttac tttaaagaat gcaaaaagag gtgtatggaa gattcgatta
    54721 gtaggagttt atattataac aggaagatat aacttatacc ttccaaatag agaactttta
    54781 aaaagtggaa ctagatttag agaggtagac cctttttaca ctataaatta tcctgcgatt
    54841 caagatgatt taataaccat aggagcctac aatactatta atggtagttt atggcaaagt
    54901 tcatcaagag ggccaactat tgaagacagg ttaaaaccag atatagtagc accaggtgtc
    54961 aatataatag ctgcttatcc tgggaacacg tatgcaacta taactggtac agcagcagca
    55021 agtgcacatg cagctggtgc tgctgcaatg tattttcaat atacttttgt tgacggtaga
    55081 tatccaaacc aagcatatgt gcaaaaaata aaaactttta tgcaagctgg tgctagaaaa
    55141 gattcaaaca cagtatatcc aaatacaaat tctggatatg ggctcttgga tgttagagga
    55201 atgtttgatg ttttaagata ggagggattt atggaaaaat cttattgtat aatttatcaa
    55261 ggtgatatag aatctgccct acaagaaaat ggaataaata ggtatatggt tttaaatagt
    55321 cagcttgcag taatatatgt acctttagat tttgatgaaa ctattttaaa taatataata
    55381 caagttgctt ggtgggaaga atctgaacca atgagcagtc taattgaaat aactaataat
    55441 gtaaataatg gagaaaccat tacaacagca gctgaaactg attatattta tgaaaatcca
    55501 tataatgata taacagggag aggaatttta ttagcagtta tagattctgg gatagattat
    55561 ttgcatcctg attttataaa tgatgatgga actagtaaag ttttatattt atgggaccaa
    55621 gaagcaaata caaatccacc tccagaaggt tttatttttg gaagtgaatt tacaagaagt
    55681 gagttaaata tagccataaa tagaaatgat gggagtttga gtcaagataa tataggtact
    55741 ggaactttag tttctggtat attagttgga aatggaagga taaattctca gtacagaggt
    55801 attactacag aatcagactt aatagtagtt aaattaaaat catatacaga tacttattat
    55861 gctggaagga taaattacag tgtttcagat tttttagcag ctataacgta tgttactaac
    55921 atagcaagaa cagaaaataa accactgata ataaatttga ctattggagt taagtcaagt
    55981 gctgtagcaa ctacttcaat actagatact tttaatatat tgagtagtgc tggtgtggta
    56041 gtagtgagtg gtgcaggaaa tcaagggaat acagatattc attattctgg cagatttagc
    56101 agtgtgggag aagttcaaga tgtaataata caagatggag atgactatgc tcttgatata
    56161 acactaaata cgaatggtcc agataaagtg ggagcacaga taatatctcc ttcaggtgaa
    56221 gttagtcatg atataaggta ttctccagac ttttatatat atagaggaaa atttaactta
    56281 gaaaatacta cttatgcaat gagatttatt tatccatata ttacctctgg aaaagaaaat
    56341 ttggagataa gattaagaga tataaaacct ggagtatgga ttttaagatt gacttcagaa
    56401 cttataataa atggagaata tgacatatac ttgccaaata aaaatttgat agctccagac
    56461 acaaggtttt tagaccctga ttcagtagct actataacta tgtatgctgc tagtgatgat
    56521 gtaattacag ttggtacatt taacaataag actgatagta tgtggattgg ttcttcgaaa
    56581 ggaccaatta gaggaagagg aattaagcca gatatagtag cttcaggtgt agatataata
    56641 tccacatata aaaatggaac atataataca ggaacaggaa caggagtgag cagttctata
    56701 gtcacaggtg ttttagcctt attgatggaa tacttagaaa aacaggataa tgtaccgaga
    56761 ttatcattgt ttacacaagt tttaaaaaca tatttaatat tgggtgctac aaaacttgag
    56821 atatatacat atccaaatgt atcgcaggga tatgggattt taaatttaaa aaatacgatt
    56881 caacagatag caaatactct atagataaga acctatgtaa aattataatt gattattagt
    56941 ttaattgtat caaatacttt agatagagta gaaaaatagc ataaaaaata aatatgaaaa
    57001 ttaaaaaaca gttttatatt ttgaatacac aaagtaaata ttttgtatat aaataggtat
    57061 aaattaagta ttgaaaagat atgatgttaa tttaaaaata attataattt agacttatga
    57121 attttatttg taaaattgct aaaactatta caaaataaat aatagaggtg atagttttgg
    57181 aaagtaaaaa ggcaagagca ttaaaaggta aagataataa aagacttaaa agttcacttt
    57241 tagttggaaa tgagtcgaca gcagcatggg ctgatgttga aaaattaaaa cctcatagta
    57301 aagtttcaat accttcaatt aagggtgtcg aagaagcaaa agactgggtt gacaatggaa
    57361 gtaaattata aaattttgga aattgaagta ataaagaagt aatgaagata agtatttatt
    57421 atcattatgt aaggtagaat tattatattt taaataacaa tcagtatcaa ttatataaat
    57481 ttattgacta aaaatttata aattggtata attttgttaa ttagcagaaa taataaaaga
    57541 taaatactgt gaagagaaga gtagatataa aatgtagttc tagcgagttg aggatggtga
    57601 aagctcaata cgaggtttat attgaagaac atcttggagt ttctaatcga aagcttattt
    57661 agtaagtaga cttagacgga aggcaccgtt ataggcttta agcattgctt aactaagagg
    57721 tcattatgac aactagggtg gtaccgcgag agtaatagtc tttcgtccct ttatataggg
    57781 atgaagggct ttttttattg aaattatatt taaaagaaat taataacaat tatactagga
    57841 ggatttaaaa tgcaaaagga atttataaca gtaaaagaat tgtacagaga aaaagaacag
    57901 tacataggga agactgtaaa agtagctgga tggataagaa catctagagt atctaaaaat
    57961 tttggattta tagagctaaa tgatggaagc ttctttaaaa atatgcagat agtatttgat
    58021 gagaaagtag aaaattttaa agaggtagga aaattagcaa taagctcatc tatacttgta
    58081 gagggagagt tagtatctac agaaggggct aaacaaccag tcgaaataca tgcaaaaagc
    58141 atagttgtag aaggggaatc agatagttcg tatccactac aaaagaagag acatacgctt
    58201 gaatatttaa gaacgatagc acatttaaga ccaagaagta atactttttc agctgtattt
    58261 agagtaagat caatagctgc ctatgcaata cacaaattct tccaagaaag aaactttgta
    58321 tatgcacatt ctccaatcat aacaggtagt gactgtgaag gtgcaggaga gatgtttaga
    58381 ataacaacta tggatttaaa tgatattcca aaaactgaag atggcaaaat agactatact
    58441 caagatttct ttggaaaaga ggctaattta acagttagtg gtcaattaaa tgccgagata
    58501 atggcacttt cttttagaaa cgtttatact tttggaccta catttagagc tgaaaactct
    58561 tatacaggaa gacatgcttc agaattttgg atgatagagc cagaaatagt ttttgcagac
    58621 cttgaagata acatggaact tgcagaagat atgataaaat atgttataag ctatgtatta
    58681 gaaaatgctc ctgaagaaat ggaatttttt aatagtttta tagataaagg attgttagaa
    58741 agattaaata agatagtaaa ctctgagttt attagaataa cgtatactaa agcagtggaa
    58801 ttattaattg aatctgggca tgaatttgag tatcctgttg agtggggatg tgaccttcaa
    58861 acagagcatg aaagatatat aacggagcaa atatataatt gtccagtgtt tgttacagat
    58921 tatccaaaag atataaaagc tttctatatg agaatgaatg aagatggtaa aactgttaga
    58981 gcaatggatt tactagttcc tggagttgga gaaatcgtag gaggttctca aagagaagaa
    59041 agagaagatg tattgttaga tagaattaat gaaatgggat taagagaaga agattattgg
    59101 tggtatttag aacttagaaa gtttggtact gctactcact ctggatttgg tcttggattt
    59161 gaaagaataa ttatgtatat gacaggtatg tctaacataa gagacgttat acctttccca
    59221 agaactccta aaaatgctga attttaataa ttatatataa taaaacttat ataactttaa
    59281 gtataaacac taaaatgact tgcatattta tctattttag agatatgcaa gttattttta
    59341 ttgtactata aatatataat ttgattactt ttattttgcg atataatact tataggttta
    59401 tgtaatagta tgtaaccttt taataaaatg caacacaatt acgtccttgt tcttttgcag
    59461 tatatagggc cttatcacaa ttacttataa actcatttaa tgttattgtc tttccaattt
    59521 tctttgtata aactccaata ctaacagtta cacattttaa aggaacatca tcacgctcaa
    59581 aattgcagga ctctataatt tttcgtaaac attctgattt aagtttagca tctgcctcat
    59641 tacagttaaa aatacatata aaaaattcct caccaccata tcgtatagca tgttcatttt
    59701 cagaacttgt aaaatctaaa attactttgg caatattttg tagacaatag tctccctttt
    59761 gatgtccata attatcatta aattgtttaa aataatctat atctatcatc attccagtta
    59821 taggttcttg atttgcatgg ctgattaatt ttttttgtag aatatcttca aggtatctac
    59881 ggttgttcat tttagtaagg tcatctgtca taactttgtc gttaagtaaa ttatttatat
    59941 gagatatttc ttcatattgt tgactaatta aaagtttatc atagtaagta aatacacgat
    60001 ttctatatag cattgtagaa acaaaaatgg caagggatgt tataaaaaat gtgttgacaa
    60061 tattactaaa ctcattgcct ggtatagttt gaatcccagg taagaataaa ttaagaaaca
    60121 aaaagcatcc tccaaaaact attactgatt ctccaaattt taatactgag aaagctgcag
    60181 ctgctaacat aatataacta aatacactta ggtcatttcc acctaactgg tcaagtagag
    60241 taattacaca accccatagg cagaaaaaag tcacataaat tatccataaa tttatataaa
    60301 acgtacagca atcctttttt ttattccaaa ggtaagaaaa tacaaacata aagacagaag
    60361 atacacaaaa tagtgttaca tacatataaa aataccattg gcgtcttggt gtgacaaaag
    60421 ggcctccttc tctagctgag attgatatca tcataattat ttgagatatt actattatta
    60481 tgcaaatgat tttacccgta tgataaagat aattgcagag ttctttctca acattgtctt
    60541 tgtgtttttt gtctagagga aatccaaaaa atttttttat tttttctaag ttcatataag
    60601 cctccattaa aaataaagca taaaaataag tattcctttc tatcattaga tatttggaat
    60661 acttcgttta tgatttaatt atatcaaaat atattgtgtg taacaatcta aaattgtgat
    60721 agataaaatt tattagataa tgtcgagtaa ctttattgat taatacttta ttataattaa
    60781 gatttacaga tagtttacta ctgaaaataa agaatagaat aaatttaaat taagcataat
    60841 aaaatataaa taataaagtt cgcattaaca aactatttaa atatcaagaa ctgttaaaaa
    60901 acaacagcaa taaacaaata aaatagataa atatatccaa ttaaggtata attaagtttt
    60961 atatgatata gttatatttg ataatttaca atagtaaaac ttggagggaa aaaatgggtg
    61021 aaaagaaaat aaataatatg aattattcac ataataatta ttccaaaaag acaggtgaac
    61081 caactagaaa aataaaaaat agccatatca ctcttaaaag taaagaagaa aaaatttgtt
    61141 ctagttgcaa taaagaaaat aatactaagg ataattactg taaattttgt ggaaatgaac
    61201 tttatgaaat agcatcatta agaccacttg aaacaaaact agacttgaaa tctaaaataa
    61261 aagagctttc atatcatgca aataaaagag gtgtattttt aacaacattt acaaccatat
    61321 ttatattgtt tattattgca ttgatattta aagctattat aactattcaa tttaatgata
    61381 ttagttattt agtaaatcca gcacatataa tcctagctct caacttgggt caaattagtg
    61441 tatctatgtc taccatgatg ggttctgggt ttataaatgc caatataggt ttgttgatat
    61501 tacttataat ccctattttg gtattattaa tatcaaacct aatttttatg agaaaaagat
    61561 gtagagattc aaaaactgta ttagcaaatt ctctaggagt tggaattttt tatggattag
    61621 ttttagctat tttatctatt tttactaatg taaaaactag ttcacatagt atgttagaat
    61681 atggatatgc attagaatat agttatgaat tttttagtgt tcttttaaat ggattcgtat
    61741 tagggtttat atgtacatac ataactactt ataaaaaaag ttatgaaaaa gaaaatatgt
    61801 atttatctct tttttcaaat tctataagaa cattcatatt aggttatgtt ttagtattag
    61861 taatactatt ggtactaact atatctgata gtagttactt aaatgaacta gatatgagta
    61921 gttattcaaa tggattaaat ttatttacta tactcccaca aatagctagt tatatgtggg
    61981 cttttgcaaa tggaatttca gtaaccataa taaactcaac agtatcaatg tttactttaa
    62041 gttcttcatc tttatttgga gatactaaat taatgtttta tgcaatggga gcattgtcta
    62101 tgttaatatt attattaaat ggatacaagc taagatttaa gtataataca gatagtataa
    62161 gacctataat agtatttagt atatattatg catttttgat gggaatattg gctttattta
    62221 gtacatttat actagatagt aatattaatt tctttaatac aactaattat ggaacaactt
    62281 taataatgca atttaaagtc ttacaagcta ttgtgatttc atttgtatat agttttgtga
    62341 tatctttaat aggatataag ctaaattcag ctgattaaat atgattggag ggaatttctg
    62401 tggaaggaaa tactaataat aaagtagatg ataaattttc taaggttaag aaaaagtttg
    62461 gtagtggaaa atttaaagtt gaattatata aagaaaaatc aaaattaaat agagagattg
    62521 atgatattca agaagagaaa gcaaagatat tcttggaaat gggaatactg acatatcaaa
    62581 aaattagaga agattctatt aatgataata gttttgataa attatgtgat gagttactag
    62641 aacttgataa gttaatttat gaaaaaaata tggaaattaa tgaaatggaa atggcagaaa
    62701 gtaatgtaac ttgtgagtgt ggttatgttg gaaatgtaaa tgataaatat tgtgctgaat
    62761 gtggtaaaaa atttgaattg gaaaatgaat gtgatgattt tataatttgt ggttattgtg
    62821 aaagtgagat agatacagag gctgaatttt gtccatgctg tggaagaaaa attataatag
    62881 atttagaata atattgggaa acctatgtat agtatacatg ggttttttta ttgaaattat
    62941 ttaaatggac ttataacagt agttatataa aatgaaaata aacaccgaaa aaatgaaata
    63001 aaatttcttt taaatgtaaa aaacaagcta atatataaaa taaatcagta aaaatataga
    63061 tgtgagaaaa aaatatgaaa aaatattgca aagaaaaagt tttcatgtta ttattaatac
    63121 ataaatcaag aaaataaatt tcagaaacga ggtaatttaa aaaatgctag ataaggtaaa
    63181 gggaagatta atagtatcat gccaagcttt agaagatgaa cctcttcata gtccttttat
    63241 aatggggaga atggctaagg ctgctatgga aggtggagct gttggaatta gagctcaagg
    63301 agttgaagat attattgaaa taaaaaaagt tactggactt cctgttatag gtattataaa
    63361 gagaaactat gaagattcag atatatatat aacgccaact aaaaaagaag ttgatgaact
    63421 tttaacaaca gggtgtgaaa tgatagcatt agatgctacc aatagagtta gaccaaataa
    63481 tgaagactta aaagaactta taaagtatat aaaagaaaac ggtgttctag taatggcaga
    63541 tatttcaaat tatgatgagg ctatcaaagc acaagagtac ggtgtagatt gcgtttcaac
    63601 tacactatct ggatatactc catatactaa aacattagaa ggtccagatt ttgtattaat
    63661 ggaaagatta gttaaagatt tagaaatacc tgtaatagct gaaggaaagg taaatactcc
    63721 tcaagattta aaaaaggtct ttgagttggg tgttcattca tcagtagtag gttcagcaat
    63781 aacaagacca caattaataa cagaaaaatt tgttaaagca attgaaatta attaaaaatt
    63841 aatttataaa ttaaaaaatt attatattta actatatttg aaaattaaaa ttggaggtaa
    63901 agatatgaga acaacagata tgaagggaat ttattcagca ttattagttt catttgataa
    63961 ggaaggaaat ataaatgaaa aagggttaag acaaataatt agacacaata tagatgtttg
    64021 taaagtagat ggtctttatg taggaggaag tacaggggaa aacttcatgc tttcaactga
    64081 tgaaaagaaa agaatatttg aaatagctaa agatgaagtg aaagaagaaa taaaattaat
    64141 agctcaagtt ggttcagtta atttaaaaga agctgtagaa cttgctaaat ttacaactga
    64201 tttaggatat gatgcaataa gtgctgttac acctttttac tataaatttg attttgaaga
    64261 aataaaacac tattataata caataataaa ttctgtagat aatagattaa taatatactc
    64321 tataccattt ttaactggtg ttgatatgag tttagaccag tttggagaat tatttgaaaa
    64381 tgaaaaaata ataggtgtta agtttacagc agcagatttc tacttattag agagaatgag
    64441 aaaaacattc ccaaataaat taatatttgc aggatttgat gaaatgatgt taccagctac
    64501 agttttaggt gtagatgggg caattggttc tacattcaat gtaaacggtg tcagagctag
    64561 acaaatattc gaattaacta agaatgaaaa aatatcagaa gcacttgaag tacaacatgt
    64621 gactaatgat ttaataacag atatattagg aaatggctta tatcaaacaa ttaaattact
    64681 acttgaagaa caaggtgttg aggctggata ctgtagacaa cctatgaaag aagcaactga
    64741 tgaaatgaaa tcaagagcaa aagaaatata cagaaagtat ttttaataaa atacttttag
    64801 tatgggagga ttagtcgaat ggtaggattt acttggatag attttgcaat actagtggtt
    64861 tacttattgg cagttttatt tgcaggattg ttattttcaa aaaaggaaat gaagggaaaa
    64921 gagttcttca aaggtgatgg aactattccg tggtgggtta catcagtatc aatatttgcc
    64981 acattattaa gtccaatatc atttttatca ttagcaggaa attcttatgg tggtacttgg
    65041 attttgtggt ttgcacaatt aggtatgttt atagcaatac ctttgacaat aaaatttttc
    65101 ttacctctat atagtaggtt agaaatagat acagcatatg aatacttaga aatgagatac
    65161 gaaagtaaag gtcttagagt acttggagct ttgatgttta taatatatca aataggacgt
    65221 atgtctatta taatgtatct tccatcaata gttctggctg gtcttacagg aatacctgta
    65281 aatgtactga taatagttat gggtgtaata gcaataattt actcatatac aggtggatta
    65341 aaggctgtct tgtggaccga ttttatacaa ggaatggtat taatagtagg tgttacaggg
    65401 acattgttct atttaatagc aagtataaat ggtggttttg gaacagtaat ggatactctt
    65461 acaactggac acaagttctt agcaagtaat gaagttatgt ttgataaaaa tatactatct
    65521 acaagtgcat ttataatatt tgttggagct ggtcttaata ctttttcatc atatgtatcg
    65581 agtcaagata tagttcaaag gtttactaca acaactgata taaagcaact taataaaatg
    65641 acatatggaa atggtgtttt atcaatggga cttgcaacag tattttatct aattggaact
    65701 tgtttattta tatactatac tcaaaaccca gatttagctc aaacagttca acaagaccaa
    65761 gtatttgcat catatatagc ttatgaatta ccagtgggta taacaggaat actacttgca
    65821 gcgatatatg cagcttctca atcaacttta tcgacagggc ttaattcagt tgctacaagt
    65881 tggactttag atatacaaac tatcataaca aaagatataa gtatggaaaa gcaaactaag
    65941 atagcacaat atatatcatt aggtgtaggt atactttcta tagctgttgc aatagtatta
    66001 gcaaatggag aaataaagtc agcatatgaa tggtttaata gttttatggg gctagtacta
    66061 ggtgttttag caggaatatt tgtacttggt gcattttgta aaaaagcaac taaaatggga
    66121 gcatatgttg gattcatagt atcagcaatt ttagttgttt atttaaaata ccgtatgcca
    66181 gaagtaactt cttgggcata ttctctaata acaatcacga cttctgtagt ggtaggtaca
    66241 atagttagtg caatagaagc taaagttaaa gcaaaagtat ctttaccaaa agctgagact
    66301 actgtttatt atgaaaaata atatttaaat gagaaattgt cttatagagt gattagataa
    66361 ataatttagt tactctataa gattgtataa tatactttgt atacaaaagt ataaaaatag
    66421 actttataat tatataggga gatagtgctt atgatatttg gaaatataaa tcatagtaag
    66481 acatattcaa cattgcatga agatttactt agatgttttg aatatgctaa agataattca
    66541 ttaatagatt atgaaaaagg tacttataca atagatggtg atgatatttt tgtgaatatt
    66601 gtagagtata aaacttgtga aaaagaagat agattctggg aagctcataa gaagtatata
    66661 gatgttcatt tgatgcttga tggatgtgaa cgaatagata taaattttat agaaaattta
    66721 gaacaaaaaa tatatgaaga aaagggcgat ttcttgcctc ttgatggtaa aaataatgga
    66781 tacatggaac ttagaaaagg tgatttttta gtttgttatc cagaagatgc tcatatgaca
    66841 ggaataaaag ttttagaaaa agaaaatatc aaaaaagcta tttttaaggt tttggttaga
    66901 taatgataat ttatataacc actagggctg aaaatagtga tttaactttc agcgattagt
    66961 gagacttcgt aaattttagt tagattacta ttttcagcct tagtttttta tgagaaaatt
    67021 gaaaataatt aaaagcttaa aattttgtaa aggagttaac ttatggaaaa tatagatgta
    67081 ttcttattaa taggacaaag caatgctaga ggattgggaa atccaaaaga gagcatcatt
    67141 ccaaatgaaa attgttttga atatttgagt actgatgaaa ttataaatat gagatgtgag
    67201 ctagagactt cagaaggtga tggtactata gcacctgcat tttcaaatga atggaataag
    67261 cttactggaa ataaagtttg ttttattcac aatgcaaaag atggttcaag aataaaaaac
    67321 tggaatcatg ataataactg gtttctaaat gacactatag aaaaatttaa tacaggctgt
    67381 actaccttaa gtaaacatta taatatagaa aataaatacg ttatttggat tcaaggtgaa
    67441 agtgatgcaa aatatggtag tgatgtattg tactataaag aaagtctaaa aaaaattgcg
    67501 tataggctta aagaagaatg tatgattgat aaaatgtttg taagtttaac tggttattgg
    67561 cttggagaag atgaatattt tattagaaca agacgaattg cagcagcaca ggagtcggct
    67621 tgtagtgaat gtgatatatt atgtgtagga agcaaaattg ctatgcagtt tcatgacaga
    67681 ggattaacta ttgatgatgt tcattacact caagaaggtc ttaatatgtt aggagaggac
    67741 ttatgtaaaa atatttataa gtatcatata accaaagaaa aaagtgtatt aagtgatact
    67801 atagacttat cagaagcaag gaaatatatc tgtgaattgg aaaagataag taaaagattt
    67861 gtgtaatata aaattttaaa tagagttgtt aatttataaa aataatttgt atggagatat
    67921 gttatgaaaa attacgtttg tatagatgtt ggaggtactt caataaaata tggttttatt
    67981 agagaagatg gatttataat tgataaatca agtcttgata ctgaagcaaa ggaaaaaggt
    68041 ggagaaggaa tattagccaa aataaaagat atagtaaaaa aatatataga ggaaaatgaa
    68101 ataagtggta tatgtatatc aactgcaggt atggttgacc cagttgaagg taaaatacta
    68161 tttgcattag aagaattaat tccaaactat aagggcatgc aattaaaaaa agaagtagaa
    68221 aaagagttta atataaaatg tgaagttgaa aatgatgtta attgtgctgg acttggtgaa
    68281 atgtggcttg gagcaggtag aggtgcaaca tcttctatat gtttaacaat aggtactgga
    68341 attggtggat gtattataat aaataataaa ctgataaatg ggtttagtaa tagtgctggt
    68401 gaagtaggat atatgaatgt aaatggaagt tcttttcaag aattggcatc tactagcagt
    68461 ctaattaaaa aagtagcaaa aataaaaaat ttgaatgaaa atgacttaaa tggaaaaata
    68521 atttttgatt tagcaaagaa taatgatgaa gattgtttaa aagaactcga taatatgata
    68581 aaatcattag cagttggaat cgcaaatata tgttatgtaa taaatccaga agtggtaatt
    68641 ttaggtggtg ggattatggc acaagaaaag ttcctaaaac caaaaataga caaagcatta
    68701 aaggaggttc ttatagagag agtttacaaa aacactaata tagaatttgc aaagagacaa
    68761 aatgatgcag gaatgcttgg agcattatat aattttttaa acaaaatata aagaatagaa
    68821 gggtttttaa tgagtatttt agaacaattg gaatcaccaa cttttaaagt cacaaaatca
    68881 gataaattat taatagctta tataaaagaa aatatagatg atgtatttta taaaccaata
    68941 tcacaaatag ctaaagagag caatattggt gaagcaacta taactaggtt tgtaaaaaaa
    69001 atgaatttta atggattaca ggattttaaa gttactttag cacaagaaat atcaactatc
    69061 aataaaaaaa atattataaa taaaaatata caaaatgatg agccagcttt ggatacagca
    69121 aaaaaacttt taagttcaaa tgtaaccact ttagagaaca cagtagagat aatcaatagt
    69181 aaggatgtac atgactgcgc aaggcttata ataaatgcaa agaaagttta cttcataggt
    69241 attggatatt ctggtataat agcacaggat tcaaactata aatttatgag aataggtctt
    69301 aattgtgtaa gttttgacag tagtcataca atgataatga tgtcttccat aatggaagaa
    69361 ggagatttaa taattgcaat ttcacattct ggtgaaacag aagagattat aaaaactgta
    69421 aagcttgcaa gagccaataa tgctaaaatt atatcaatta ctgaaaataa aaattctgaa
    69481 ttaaaggata tttcagatgt acacttatct tatgtatcgg gagaaacagt actggaaacc
    69541 ggttcaatat cttctaaatt agcacaattt ttcattattg atttagtata tacacaggta
    69601 gtaaaagaac tttctaatga agctattgaa aggaaaatta aaactacaaa tgctataaaa
    69661 ttgtttaaaa atgaataaaa taagaaattt gtaatgtttt atagagctct cttaaaaatt
    69721 taggaatgga gagatattac atgtctaaat taaaagatta taatattgat aaagtaataa
    69781 tttttaataa tttaagtact aaaacgaaga atcaacttaa agaaaaagtt aaactaaaaa
    69841 gattacaaaa aaaagaaatt ttattttatg aaaaagatag cttagacagt gtttatatac
    69901 ttttagaagg aaaggtatct atttttaaga taagtgaaaa tggagagaga aaagtaattt
    69961 tcatattaaa tagtggggaa gtcataaatg atattacact tgacgcttct aaaaactcta
    70021 ctgttggctg tgaagcattt gaaaattcaa ttgtagctta ttatagtatt gaagaatttt
    70081 tagaaattat gcaaaatgat tttcaactta caaaggatat tatttcttat atggaaagaa
    70141 gaataagacg tttatataga cagttgaaaa attctatatc cataaaagta gataaaaaac
    70201 ttgcagcaaa attatatagg ttaagtagag aatttggagt ttgctgtgga gaatggactt
    70261 tactaaatgt aaatattaca gttacatatt tagctgatat gcttggctgt aaaagggaga
    70321 ctgtttccag aatgataaag gtattgcaaa aagaagaact tattaaaatt gacaataaag
    70381 gtttttatgt taaagaaact gaattatcaa ggtattttaa aaataattaa ttatgattaa
    70441 gtaagagtat tattagaatt ttaattaaat aaaaatatta ttaagagaat ctatttatag
    70501 attctttttt attgtctaat ttattaattt ttattttacc ctattgaatt gtataaaaaa
    70561 acaaataaga ttctttttta ttcaaaaaat tgagtagata gtagttttat tgtacttaat
    70621 gattgtattt aagtgattag atttgaaatt gaataatgta gataataaaa aaatattaac
    70681 agaacttaat aaactccatt aatatttttt ttattatttt tgaaactgtg ataattatca
    70741 cggaagcata gaaaataaag tgttaagatt taaacaagat agcaagtaag taaaactaaa
    70801 atataagaaa ggtgataaaa atgaaactaa aattagattc atctaagttc aatgaaggtc
    70861 tgaattatct aaaaaaagac tacaagatat ttgcaccagt tttaatccca tttaaaggta
    70921 ctttttcaga tactgatgtt ataagataca aggaggtaaa cgcttttgaa gaaatggaat
    70981 ttgctaagaa agcaaacttt tcaccaaaag aagttgtact tcccataaat caagtattgt
    71041 tttattttac tgaaaaagag tttaaggaaa gcgatttaga caacaagaaa attttaattt
    71101 tcttaagagc ttgtgacata aatggtataa aaagacttga tgaaatatat ctaaataatg
    71161 gtgaagaaaa agattatttt tataaaaata tcagagataa agtaaagttt gcacttgtag
    71221 ggtgtaagga aagttttaga aactgttttt gtgtgagcat ggattctaac aaaactgata
    71281 attattcaat tggattaaat atagaaggag atacactgta cttagatata aaggatagtg
    71341 aatttgaagt atttaatgga gaagcaactg aatttaatgt tgattatgta actgataatt
    71401 taatatctgt agatgtgcca gagaatattg attcaaatga aatgatagga aattcaatat
    71461 gggatgaata tgatacaaga tgtataggat gtggtagatg taattttgta tgtccaacat
    71521 gttcatgttt tacaatgcag gatattttct ataaggaaaa cgaaaatgta ggtgagagaa
    71581 gaagggtttg ggcttcttgt caagtagatg gatatacaga tatagcaggt ggaaattctt
    71641 ttagaaaaaa acaaggtgaa agaatgagat ttaaaactat gcataaaata catgatttta
    71701 aaaaaagatt cggatatcat atgtgtgttg gttgtggaag atgtgatgat gcctgtccgc
    71761 aatacatatc attttcaaag tgtatagaaa aaataaatga tttagtaatt agtaaggagg
    71821 aagtataaat gaacccatat ataccagtaa gtgcaaaaat actagatata gttaaacata
    71881 ctgataaaga atggactttt agagtaaact gcaatacaaa aggtgtattg cctggaaagt
    71941 tttatgagat atctatacct aaatacggtg aaagtccaat atcagtatca ggatatggag
    72001 aggattatat tgactttaca ataagaaatg taggaaaagt aactagcgag ttgtttaatt
    72061 ataaagaagg agattctttt tttataagag gtccttatgg aaatggattt gatgtatccc
    72121 tttatgaagg aagagaaatt gtagtagtgg caggaggaag tgcattagcc cctgtaagag
    72181 gaatagttga atatttttat aataataaag aaaaatgcga aaagtttaaa ttaatagtag
    72241 gatttaaatc accaaaagat attttatttg cagatgattt aaagagatgg agtaaaaaac
    72301 ttgatatttt agttactgta gatggagcag aggaaggata tagtggtaat gtaggattag
    72361 taacaaaata cataccagaa ttggatatga aagatataaa caatacttct atagttgtag
    72421 ttggacctcc aatgatgatg aaatttacag tagaagaatt tttaaaaaga gatttagatg
    72481 aaaagaacat ttgggtttct tatgaaagaa aaatgtgttg tggaataggt aaatgtggac
    72541 attgtaaaat ggatgatact tacatatgtt tagatggacc agtatttgat tattcatatg
    72601 ctaaaaatct tattgattag ggggtgaagt ttgtgataag agatttaaat accaaaaaag
    72661 taatgaaaaa tgcttataga attacaaaaa caaaatatga gacttcttta agagttagag
    72721 tacctggagg tcttattgac cctgagagtt tagctgtagt ttctaaaatt gcaagtgagt
    72781 acggcaatgg acaggtgcac ataactacaa gacaggggtt tgaaatatta ggaataaata
    72841 tggaagatat ggaagaaata aataaaatta tacaacctgt tattgaaaaa atgaatataa
    72901 atcaaagtga gaaaaacgca ggttatccag cagcaggtac aagaaatata gcagcttgta
    72961 taggaaataa agtctgtcca aaggctcaat ataatacaac tgaatttgct aaaagaatag
    73021 aaaaagctat atttccaaat gatttacatt ttaaagtggc tttaactggt tgtccaaatg
    73081 attgtataaa agctagaatg aatgactttg gaattattgg tatggcttta ccaatatatg
    73141 aaaaggatag atgtgtaaat tgtggagcat gtgtaaaaaa atgttctaag atatctgttg
    73201 gagcattaaa aacagaaaat aataaggtgg taagagataa agataagtgt ataggctgtg
    73261 gagagtgtgt actaaattgc ccaacaaatg catggactag agatgagaaa aaatactata
    73321 gattagctat aatgggtaga acaggaaaga aaaaccctag actagcagaa gattttcttt
    73381 tatgggtaga tgaggacagc ataataaaga taatacttaa tacttataga tatgtagaaa
    73441 agtatataga taaagatgca ccaggaggaa aagaacatat aggatatata attgatagaa
    73501 caggatttat ggaatttaaa aaatgggcac ttgaaggtgt agagcttcca gaaataacta
    73561 aaatgtacga aaacatatac tggagtggaa ttaaatatct ataaattgag taaaacatat
    73621 atttgaataa attaaaattt aatagaagga agagggagtt atgcataaag aaactttgga
    73681 taaattaact aatgcagcta taaataagat aaatttatta aacacaagta aagtaaaata
    73741 tttggtatct tctgcgtttg caggacttta tgtaggtata ggtatacttt tgatatttac
    73801 tattggagga cttttgactg atgcaggttc acctatgact aagatagtaa tggggttatc
    73861 atttgcaata gcactaagtt tggttataat gacgggaaca gagttattta ctggaaacaa
    73921 tatggttatg tctgctggta tgttaaataa aggtgtaagt ataaaagata cttctaaaat
    73981 atgggcatac agttgggtag gtaatttaat cggagctttg gttttaggca ttatatttgt
    74041 aggaactggt ctagtagaca aaggacctgt agctgaattt tttgcaaata cagcagctag
    74101 taaagcatcc atgccattta cagctctttt ctttagaggg attttatgta atattctagt
    74161 atgtgtatca gtgttgtgtt cgtttagaac taatagtgat actgcaaaaa taattatgat
    74221 attcttatgt ttatttgctt ttataacatc aggttttgag catagtgtag caaatatgac
    74281 aatttatagt gtttcattat tttcaccaac aataagtaca gttactattg gaggagctat
    74341 ttataattta gtagctgtaa cacttggaaa tattgttgga ggagctttat ttatgggtct
    74401 tggaacttat atattaggta aagaaaagct taattaaatt tattgtataa attataactc
    74461 tcttgataaa tacattttta gtattgttga gagagttatt tttatatttt tgaaaaaata
    74521 tttgaaacaa aaaaaatatg ggagcatacg aaaatatatc ggttatatat ataaaaaata
    74581 cgtttatata tgaattatat agttctaaat ataaaaaaag ttaaagtcct accgtaaaaa
    74641 agcgcatttt gaataagata acaaatatga taaaattggt cttgttgaaa tttttagggg
    74701 ggatatgatg acaaagaaac tagtaatatc aaaaacaaag ttgtttattt ttataatagc
    74761 atttatttct atatttatat atttatttgg aagtaaaaat acacttatag gtgtaggtat
    74821 agtaacagct atgctaacac tcttggaaag agatttaact atttctccaa ttaaaaattt
    74881 actgaagtat ttagcaataa atataatctt aggaatacta tcattttttg cagtacaaaa
    74941 tatgtattta ggagttttat taaattttat agcattattt ataatagggt atgtatttag
    75001 ttatgactta aaaagagcag tatatgtacc ctttggtcta atgtatatat ttatggtaag
    75061 tatacctgta ggaaaaagtg aatttcctat gagattgtcg gctttagctg ttggagcagt
    75121 tataataatg attgctcaat ttgttatgaa tagaaatagg atgaaaaatg tcggagataa
    75181 agaactgata tctatatgtg atgaattatt agaaaaaatt agtcttttaa aaaatactat
    75241 taatgatgat agttctataa ttaaatctag tatgagaaaa atagatagct gtaattatag
    75301 aataaattct atatctaaaa atttgaaaat ggttattttt gataatagaa aagatgactt
    75361 ttatatcagt attagaggta tagacataat gaatatttta ttttcattag aaagaataag
    75421 tttaatcctg gaaagataca aaaaggatag taaagaattt gaagatgaag atataaaaaa
    75481 tattttagaa gaaagtagca taaatagtaa atcagatgtt ttagttgtag caacgaaaga
    75541 aataaactat attaagatgt gtttagaaaa taaagatacc ataactgata aagagatatt
    75601 aggatttaga gattatgtta tttctcagga tactaaaaat ataaatttaa aagaaattta
    75661 tagtgtttta gaaaatttat atgagttttt agtagattat aaaaaagtaa aatcaagaga
    75721 cgaaaagaaa gctgaaagaa aatcaaagat acctcatgaa tttaaacgac tatcaatata
    75781 taagaaaaac tttaatttaa attctataag attttcttat gcagtaaaga ttgctttagc
    75841 tacagctgta gcgggtttta ttatggatta tttccattta agagatggaa gatggataat
    75901 gttaactgtc ttttcattaa cacaacctta cgcagaaaat tgtatacaaa ggtctagaaa
    75961 aagaatagaa ggtacattta taggagctgt aatatttatt gtattatttt caataataaa
    76021 agatagtaca ttaaggtcgt taatagttct tttagcaggg tatataaact cttatgtagt
    76081 tgattataga aaattaatgg tatgtgtaac tgtttcagct cttggttcag cagtagttat
    76141 gggagaccca aatgttttaa caataagcag gatttcatat gtagcattag gagcaattat
    76201 tgcattaata gtaaataagt ttatattacc atatgatgct aaaacaggct atcaacatgt
    76261 tatagaaatg tataaaggaa tagttaaaaa tataattgat gaagtcaata agtctataga
    76321 aaatgtagct gatgtttact atataaaaaa tttattattg ataccatcac ttattgaaga
    76381 tagacttatg ttgataaatg ctatatataa agataaacat caagaggatt ttttagaaaa
    76441 tcagaaatta ctaataagta atatgtataa tctatatata aatgttaata aaaataaaat
    76501 aaaagatgaa gatgttgaaa aaatattgag agataccaat tatatttcaa attacaatgt
    76561 agataagtat gatgaaggaa gaagtgtaat tttagagagt attgtaaata ctaaaagtct
    76621 aggtgacaag ataatatgtc taaacttact gcaaactcta aatggtgtca aagaaatgta
    76681 tagaataagt aatataacta aagtttcaat aaaagaagca gcttagatat taaattatag
    76741 aatagccata tatttaaaat aaagtatatg gctatttgtt ttataaaata ctaaaagaaa
    76801 ataatatata aaaaacaaaa acatatattt atgaatttta tatgaaaata gttaaaatat
    76861 tgattttttt cattggttga aaaaacaatt tacttaggat aaactataat agtattcaaa
    76921 tattatgggg gttagattta tgagtaaaga tgtaaaggta aagcgtaata agaaatatga
    76981 tacatttagt ataactatgg aacttgagag acctttactt tatgatgagg ttgagaacaa
    77041 agatgataaa aataataata gaaaatttaa cataaagaat aagatattta agggaatagg
    77101 tatactattt gtattgattt tgcttggctt tttcatatgg ataaataaaa catatgaacc
    77161 tcaaaagctg gcaaaagaag ctctagtcag caatagtaaa gttgaagtaa ctgtaaatga
    77221 aaacatttca tttacaccaa aaggaagaaa agtttctaaa ggtttaattt tgtatccagg
    77281 agctaaagta gaaatagaat cttatgctcc acttgcaaga aaaatagcag aaagtggata
    77341 tgaggttgta atcgtaaaga tgccacttaa tctagctata cttggaacta ataaggcaca
    77401 gaaagttatg gattcttaca acaacataga acattgggta ataggaggtc attcattagg
    77461 aggagttgca gcatcaaatt ttgcacgtga taataaatta attgaaggcg ttgtattttt
    77521 agcttcttat ccaatgggtg atgagttgaa agaattagga aaaaaagtta tatctatatg
    77581 ggggagcaaa gatggagtag tcaattttaa aagtcttgta gaatcaaaac aaaaacttcc
    77641 tgatgataca acttatgttg agattgaagg aggaaatcat gcacaatttg gagattatgg
    77701 aaaacaaaag ggagataatg atgctattat aagtcaagaa aaacaactta atattactgc
    77761 caatagtatc attaaatttt taaagaatat atcttaaact aaaatttaaa aaatataatt
    77821 ttataagtta ttatttaaag taaatattaa taatataata gaatgaatat tttattacaa
    77881 gggggatttt aatatggaat acgatatgcc actttacaga ccaccaagtg aggcttatag
    77941 tcttataata caagtcacac taggatgttc tcataacaaa tgtacgtttt gtagtatgta
    78001 caaatctaaa aaatttaaaa taaagccact agaagtaata aaaaatgaaa ttgatatatt
    78061 tagaagatat tataagcatg tagaaagaat attcttagca gatggagatg cattgataat
    78121 accaatggag aaattaagag atattatttt atatataaaa gaagtttttc cagagtgtga
    78181 aagaattact atgtacggct ctcctaaatc tatagagaaa aaatctgatg atgaacttaa
    78241 agaattaaga aaacttggtg ttaaaatgat ttatctaggg cttgaaagtg gaagcaatga
    78301 agtattagaa gatattaaga aaggttttag tagtgaacaa ttaataaaag ttggtagaaa
    78361 agttaaaaaa agtgggatta aattatcagc cactgtaata gcaggtcttg gaggaactaa
    78421 aaaaacacat cagcatgcag ttgatacagg aaaaatgtta ggagcaattt caccagatta
    78481 tgtgggtgta cttagtttaa tggtagaacc taatactgaa ctttacgatt tacttaaggc
    78541 aggagagttt acaatactag aggataaggc tgtgttacaa gaaattaaag agatgataaa
    78601 gaatatagat gctaatgaaa aaattgtatt tagaagtaat catgcatcaa attatgctaa
    78661 tttaaaaggt gtattacctg atgataaaca acgtctaatt gatgaaattg attactatct
    78721 aagtaaccaa aagctaaaaa gagaagaata tagaagacta tagtttttga tatatcaatc
    78781 tatattgata ggtaaatact aagattattt agatacaaaa aatcatgcta cattaatagt
    78841 tgcttactat tgatgtagca tgattttttt attaaaaaac aaaatggtat attcatatat
    78901 aatatatcaa taataatgaa aatgtgaact agtttaatag aattttacct attataataa
    78961 aaacaggaat ttaaataata aaaagtaaag taacttttta ttaagtaact ttacttttta
    79021 ttttatttaa tgttgtatta aatttatata tattaaaata atatacaaat tttgaaaaag
    79081 ctcttataaa atgattatta aagtaaagtt taatataaat aaattataaa atattaaact
    79141 aatttatcaa tatttcctga ttgaatatgc ttaatgtttg attgaatttt tttatatggc
    79201 caatcccacc atttaatttc taataatttt gaaattgtat cattattaaa tctctttttt
    79261 ataatttttg caggtactcc tccaacaata gaatacggtg gaacgtcatt agtaactact
    79321 gctcttgttc caataattgc accatcacca atatttacac ctgacataat tatagaatca
    79381 taacctatcc acacatcatt tccaattata atgttaccct tattgtccca agcgtccgtt
    79441 atatggttga tatcgagttt ccattcttca taaaagatgg gaaaagtata tgtggatttt
    79501 gatttcattg agtgatttcc actagtcatt agaaattttg cattacatgc tattgagcaa
    79561 aacttaccaa ttattaattt atcattgttt atagggtaat gatacaatac attgtttttc
    79621 tcaaaatctc ttgggtcatt ataaaagtca ttatatattg tgtaatctcc aacttctata
    79681 ttttcccttg ttattacatt tttaagatat acagtttgat aatcattact tcttggatat
    79741 accttatttg aacttggaat agtcatattt aaatctctcc tttaatatat attatgtttt
    79801 tgactattcc actactacaa tacatctctt cacagaatca ctccttgtta agaatcttca
    79861 taagataatg gcgtcctttt ttagtgttaa gtcggatatt ctttcttagt acattctttt
    79921 tagcgtataa ctgacaaact gtcattttct taccaatagt gtcaatgcta tcatcaaggt
    79981 cattttgtat tgctttttcc ttatccctta atgatatatc atcacgtttt ccctttgggg
    80041 gtttatcagt aggtacaagt ttccaagagt aaacaaattg tgctttacca aatgaatata
    80101 catacttata tgcgtatctt ccatcttttc tctggctctc tccagttctt aaaattctat
    80161 ctttattatc tcgtcttttt tcagttgaca tgataagaag ctcctctcaa taaccgaatt
    80221 gagcttgata cgtcatatga atattataac atataaggct caatttcatt agataacgtc
    80281 aagtgtatca acgattttct caaattgttt tctctttatt tgaatgcgat tgctattcat
    80341 aatgacccag ccagaatcaa ggttttcatc agcaagttta cgtaattttt tctctccaat
    80401 acgaaagtat tttgacgctt attcaatcgt caaggtatat ttttcccaaa taggtacatc
    80461 agtattgttc atagaatgta tcactccttt ctatgttcgt agttcagaag tagatagatg
    80521 gctttggtaa gctccattgg tatttcaacc aatcccattc ttatgggcag aaccgtgtca
    80581 tacaggtgta tcattatact gtggtacagg ttacggcaaa acaaccgttc caaaggttat
    80641 tcacaaatca ctatatagta gctctcttga ttaaggtcat ggcgtatagt atttgttggc
    80701 tatctgtatc acaaacgtat ctatctactt tattcagttg tcaaagaaca gtcggcattg
    80761 atttgcctat aaaaaatcac aatagcttat gggcttttat agacaaataa ttagtgattc
    80821 tccatgtacc aaatagcttg atacatggag agagggcgag attaagacaa attaaatctc
    80881 aaaatagcag tgattagttt tgttcctagc attcgctttg tttcttcttc tacatgaata
    80941 tagggatttc cgtattcatc aaataaagtt ttctgtgaaa gacgaatgat atagtgttca
    81001 aaatgtttaa gtattacgtt tattgcattt acatcgccat gtacagcaag taaaataatt
    81061 gtaaaaggta atacttcttt agtaggtttc atttcaatgt tcctgcatga gttctttcaa
    81121 ctcttttagt gcatttgttt tgtgataatg tatggtattt tctgctaaat ccatcaatgt
    81181 tgctatgtca gcatttgtca tgttgagaaa gaatgaaagt agaattatat cttgttttct
    81241 ttttgtaagt tttccgattg cttgtgccat taatacatct tcaattcgta tgttgtagtc
    81301 gtaagaaacc atacagatgt agttagaatc ataatcatct aaaatactca atttacttaa
    81361 ttctgcaagt gacaaagatt cgagagatac caaataatca tttctgcgtt tattttcgtc
    81421 ataaatatct ctagcatagt tacgcaaaac tgttttacag aaactgtcga attgatgttc
    81481 aattgtcttt tcatcaacca taagatttca ccctctttcc atcgcaagga gttaaataac
    81541 cttgcttact cctatcacgg tgggagggcg ttaaatcttc gataaatttc aaaaaaaaag
    81601 agaaaaaaat acacactctg cctatagggc aaaaatgtgt attctttaaa acgtatttaa
    81661 tttatattgg ttgtaagcca ccaccatatt gatgtatgga aatatgcatt ggctagcttt
    81721 tcataaaaat acaaagatgc ataagaaatt aatagagggg aaaaacttat tctgttatta
    81781 aaaagtaggt taattctaat aaatgctatt gcggtcgaat taaactatat acatggcaat
    81841 caatcatgtt tctattcttt attatgaatt gtgacagtat tcttcaatct taaagccaca
    81901 ttttacttga ctctctattg taggaatata cattagcatg aattttgtta aggttgtatt
    81961 tttcaaaggc aattttgcat atttctttta ctgcatttgt tgctatccct ttgccccaat
    82021 agttcctagc aagtcagtaa cctaattcag catttttatt aatagaggta tcttcttcaa
    82081 atgttaggct gatacttcca atagcccttt cttgataaaa aatagcatag ctaagttgtt
    82141 taacatctga agttgtcaaa caaaaactaa taaaatctct agcgttttgc tacctataag
    82201 ggtgggggaa gttgtcacga agattgtttg caatttcttt atcattcgca aactcaacta
    82261 aatcggtaat ataagcataa ttccactcta taagttccat ttttcttctc cttttatgaa
    82321 acagctacaa gaaatctttc taagccaata ccacttatca aacagcgttc ttttttattt
    82381 ccaagcattt gataagtcca gtcaacaaat ccaccgtcaa ctatttcaac ggtttcattt
    82441 ccttgtttga ccgataattt gaaattgata cctttataat aattattatc aacatcatca
    82501 ttatgaattg ataactctat attaggaaaa ataagtttga tagtttcaac cattctgtcg
    82561 aaaaatccgt cattatcttt gtatccgttt cttttgcgta gaataataga gaaacgaaca
    82621 ttttctattt cacttaatat atctttataa tagtttaagt gcttttctaa caacattttt
    82681 tcacaagtgt aagaccctgt atctatacca gatgaaacca tacaatataa gccaaaatgt
    82741 gaatataaac ctttacctac aaaattttgt gctcttatta ccctagcagt agtagccatg
    82801 tggataggta tggtattatt ttcttctttt ctttttaatt tatcagcaat aattattgcc
    82861 aacatatttg aggggtctgc aagtgtttct gtccctcgta gagcacttac tacattatat
    82921 tgatctacgg agccaaaaac gctacagcta cctaatggag cagagggaga aagcataatc
    82981 gtcttgatat ccatattgaa tgcttttctc aataattttg actccaaagc atgaatttct
    83041 tctggatcta taccgctagg gatagtaaat ctatttagtt ggtatgactt tagaatatca
    83101 gttgcagtta aagtatttgc ttgtctatca aacacttcta atagaagtga gttcaaatct
    83161 gattttgata gagctgataa cttatcaact atattcttat ccccaatttt atttgtaatt
    83221 ctacttaaaa tatcactcat agttatcctc catactgtta ctttcaaaat aattatagaa
    83281 ttgttacaac aactaatcta ggtcatttga ccaaaaatga aaaaacactg aatatttact
    83341 attcaatatt tttttacttt gggtttattt gattaaatct tgaaaatact gtaatgcaac
    83401 cctttcagct tcaacctttc ggacaatagg actttgtgat aattcctctc ttaattcaag
    83461 atacaggttg atagtagcct gcaatgcatt aacacagctt ttatattcat ggttagcaac
    83521 cgtcttttct aatgcttcaa tatattgggg tgcgtccaat tcaatttttc taactccacg
    83581 aggtaattta cctaagagaa gctgtattaa cgaggcaagt actgattgtc ttaaaaaaga
    83641 taaaaaatca attgtttcaa atagttctct acgtccaatc ttagtgcaag tgtaatgaac
    83701 ccaaatccaa aatctatctt caatccactg taagtctgga gttgggaagt atgcttcttt
    83761 tttagaataa atttctgtta tgcagttgtt ttcttcatag agaatagctg aatcttcaac
    83821 cctatctagt aaaccatcta aggttgtaaa cttaaaatca acatgaagaa caggggaatc
    83881 atagagggaa attatcaatc gaggttctcc aacatgttcg ccagtaaaag cagaaactaa
    83941 tgttccaaac tgttccacaa taagtaatct ctcttcgata acttcttcat aggatttatc
    84001 atctgctaca atcagaaaat ccaaatcaga atattcatct aacgattttg tgatataaga
    84061 accgctaatt gcaagtccta ttatcctact atctcttttt gaaaattcta atacgttttg
    84121 tatcatttcc tgttgtgttt tagtcataat ataacacctc tcactgtctt ttattagcta
    84181 tcaatccaac ataattatgc ataggagtat tatataatga agaaataaaa aaaaataggt
    84241 cacttgaccc gaaataaaaa agtattgaat aattattatc caatactttt ttgaaattat
    84301 acttatgttg taggaagatt ctttaatacc tctcgctgat taattacact gaatcttttg
    84361 ttttcataag caattagatg ttcttgtaca cattcattta tagcacgatt aatgcttcta
    84421 atcggagcgt ttacttcgct agcaagctgt tttttggtaa gcagatttaa ttcacttcta
    84481 taatagtgta aggatataca cctcagaagt cgttctttaa cacttaaaga tgataattct
    84541 tcaattaatt cagcatttac aactaattta ctagatattt ctcgaatcag aaattttgta
    84601 gcttcaaaat ctccccttaa ccagtttaaa aaatcatctc tatgaagttt atatacatta
    84661 caatctgtga aagcgacaat ttcaacgggt tttttcccat cattaaattg ttccatttct
    84721 ccaaaaaaac taccacttct ataaagatga atagttgaga tgtttccttt cacatttaag
    84781 atgaatgctt ctgcaatacc gtcaatcata agaattacat attcattttc ttgctcagca
    84841 aaaagaatag tctctttctt tttatacgtt tttttaacaa gcttcttttt aatatcttca
    84901 ttcataaaat caattaaatg catattcatc tctccttacc aaatctttga ttaaattata
    84961 tcatctttca attagttagg taagatagta aaaatatatt tacatagaaa ctattccatt
    85021 attattttcc agttgtcatt tttctgcaaa aacttatcac tgtcacgact ggagatacca
    85081 gtaaggaatg ttaggatatc aatcgctaaa ctttcagaat cctttttacg gctcaaaatg
    85141 acgtaatggt caagcaatcc tttgttttca tcatcactgg ttaaattgac aatgtttatc
    85201 tcatgggcta tttctggtag cgtttctttc catttgccgc gttttgcttt agggtcaact
    85261 atgaccgctt gtccgccgaa tagtaccgca tagtaaacaa gcatattgtt actgaataat
    85321 ttaccaccac ccaatgaacc gataaacgca gacgctaacg cattggtaat agaacccttt
    85381 accccttgac tagcaagggc aggttttaga tagacattct ttccagtgtc cagattgtag
    85441 ccaagataga tatcctcatg ttcgccaagg gtttgtgtcg ctccaaaacc aagtccagcc
    85501 aaaaaatccg aagtgacata ttgaatataa tcattgatgt aacgtttgct gacaggaata
    85561 aattcgccat gcaatcccaa catatcccca aagggacgca cgagcttgat tgataagtcg
    85621 tcgtaaaagt ctttgacttc atcacaacgc ctttttagct cgtcaagatc ctttgtagat
    85681 acacggacaa catagctaag tttatacata gattctttac agttaaaagt ctctttttaa
    85741 aaaaatgtat aaattataaa tcaaaatata gatactatca atgacccact aggtgcaaaa
    85801 agcattgata tggttaaatt atcagacgct aaaagctggg ctatccgaat gagtgaagat
    85861 gggtgtgctt ataaatccat tgacaattat aagcgctcac tgaaagcgtc gtttttcatg
    85921 gcaattcaag acgactgtat tagaaagaat ccatttaatt tttagttaag tgatgttttg
    85981 gaagatacaa cagaaccaaa aatcgctcta acaccagaac aggaaatggg cttattatcc
    86041 tttatgggga gcgataagac ttattataaa tattatgatg atgttgttat cctcttagaa
    86101 acaggactac gcatttctga attatgcggt ttgactttag aattagatat gaaaaataaa
    86161 gccattatgg tagaccatca attgttgagt gatactaaga ttggctatta tgtagaagtt
    86221 ccaaagacca cacagggtaa gcgagaaatt ccaatgactg aaagagctta tcaatcattt
    86281 gaacgtatcg ttgaacggcg taagaacgct aaagcagaac caatcattgt tgatggttac
    86341 cagaacttcc tatttcttaa acaagatgga ttaccaaaac aagcaactca ttatcaaagc
    86401 atgttaagag cgttagtcaa gaagtataac aagacccatg aggacaagct accaaagatt
    86461 acgccacata cctttaggca tacactctgt acaaatatgg ctaacaaagg aatgagtact
    86521 aatatattgc aatatattat ggggcataaa aacattacta tgacgcttgg ctattatgca
    86581 catggttcat ttagttctgc gagagctgaa atggaacggt tataatccta gtagtatgac
    86641 tagagtttac tacttattta ctacttttga tggcattctt atacggttaa atgccaactt
    86701 atgcaaagta tcttccaaaa agaaaatgtc gatactcctt gtagatacag gatatatcga
    86761 catttaccaa cttatgaaaa gataatcaaa tttaatataa gtttttcaaa taaaaagata
    86821 ctaaattctt tttagagaat ctatataaca ttacttaagc ttggttgggg tataaacaat
    86881 tgagtagcta cttttgtatg gtgttcaact ttagtcaaaa ctcttaaata atgcaaatga
    86941 tttagattca ttaatattca atctcttcat aagtagtgaa attcgagtta tttctgcata
    87001 attatactat gatttataga ttaattctat tagcatccag aataatattg tttatatgag
    87061 taaaatttaa agaatattgt gaaaattata gaatgttaaa ataaatttag gtaaaaaaaa
    87121 gtgtttgtat attatagagt accttttcta aaatatgtgg gattgtttta agaattagaa
    87181 taaaaattat ttttaaatta gtatttttat aatgtatagt atttttaatg tataattaaa
    87241 ttgtgcagaa aatagaattt aaagtcctat agaatttctg attatggagg agaatatgaa
    87301 aagaaaagtt caagtaaaaa atattactat tggagaaggt agacctaaaa tttgtgtacc
    87361 aattattggg aaaaataaaa aagatattat aaaagaggct aaagagttaa aagatgcatg
    87421 tttggatatt atagagtggc gagttgattt ttttgagaat gtagaaaata ttaaagaagt
    87481 taaggaagtt ctctatgaat taagaagtta tatacatgat atccctttat tattcacatt
    87541 tagaagtgta gtagaagggg gagaaaaatt aatatcaaga gattattata ctactttgaa
    87601 taaagaaatt tctaatacag gtttagtaga tttaatagat gtagagttat ttatgggaga
    87661 tgaggtcata gatgaagtag ttaattttgc tcataaaaaa gaagtgaaag taattatatc
    87721 taatcatgat tttaataaaa ctccaaaaaa agaagagatt gtatctcgtt tatgtagaat
    87781 gcaggaattg ggcgcagatt tacccaaaat agctgttatg ccgcaaaatg aaaaagatgt
    87841 gttagtgcta cttgaagcta caaatgaaat gtttaaaatt tatgcagata gaccaataat
    87901 aacaatgtca atgtcaggaa tgggtgttat aagtagattg tgtggtgaaa tatttggttc
    87961 tgcattgact tttggagctg caaaaagtgt ttcagcacca ggacaaatat catttaaaga
    88021 gttaaattca gtacttaatc tattacataa gagtattaat taaattaatt atata
//
LOCUS       DAGSRM010000007        78606 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.007, whole genome shotgun sequence.
ACCESSION   DAGSRM010000007 DAGSRM010000000
VERSION     DAGSRM010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 78606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78606
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.007"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            174..1679
                     /locus_tag="MJE33_000547"
     CDS             174..1679
                     /locus_tag="MJE33_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBY9646516.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTMPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELIVSNNV
                     DDVGESEVIDINPNNYLKKTLINYSESTLNLYAAVESIRGQFIDPFKIPLIIYDDSWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSSNQFVCEYGITIYNIPILYKSNSIGV
                     IRGGYVLLSDLNLDTEHNNLYDIPEGAARSIKRLLKQISKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMASIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIKNYLNLQKLRYGDALKVKYLIPEKLYNLSVP
                     FNFIQPIVENAFTHGFRDIDTEKRIEIIARLDSQYAIIEIHNNGTILDGNNIDKIKAG
                     IRSNNGHGLSLIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPIENYKEYV"
     gene            1793..2491
                     /locus_tag="MJE33_000548"
     CDS             1793..2491
                     /locus_tag="MJE33_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBY9646517.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIVGKATSGDEAVDKI
                     IGLEPDIVFIDIQMPIYNGLEVIEKTSHLSKKINFVVITAFNYFEYAQKALRLNVKDI
                     LLKPLDMKEFTKSVEKIIGYQYTNNDLLNEILEYINLNYSNDLKLNDCARLFLTNPSN
                     ISRIFKSNLNTTFISYLNHIRIEKSIELLENTTKSINEIAEIVGYNSLNNFYKNFKIE
                     KGMTPKVYKLNTKN"
     gene            2776..3864
                     /locus_tag="MJE33_000549"
     CDS             2776..3864
                     /locus_tag="MJE33_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646518.1"
                     /translation="MSDKVNMKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGL
                     IGALISMVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILI
                     VMISGAGSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAV
                     GLISLFKHFDGLSMVGEAMKNIEVTKASESPIVSGVIYNTLNIIVMIVFLTGMGASIE
                     SKKDAFWGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNISPIVGVCFSV
                     ALILGIYTTAVPLLWSVTNRIVEDEHPKFKLVTTVIAILACIGGFLPFGKLVNILYPY
                     TGYMGLLILVCMVYKAITCRKKSTQSQE"
     gene            4103..5293
                     /locus_tag="MJE33_000550"
     CDS             4103..5293
                     /locus_tag="MJE33_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBY9646519.1"
                     /translation="MHELILISEKYKEDILDFFTDIHMHPELSFKEFRTTKAIKDLLV
                     SLDIEILDLGMETGVVGLLKGKYDGPTVALRGDIDALPIYEEVDIKYKSRYDGIMHAC
                     GHDIHTSCLVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAI
                     FGLHNHPDIPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMG
                     IQTIVSRNISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLEN
                     IVCEIARGYQATAKLDYLFDLPAVINSKEMYAIACKSVCDLYSEDAIVDPIPSTGGED
                     FSIFMEKAPGFFYWLGVGNKEQDCIYQWHNPKFKADKNSILVGTNVLCQSVINYMDKL
                     KNKI"
     gene            5384..6886
                     /locus_tag="MJE33_000551"
     CDS             5384..6886
                     /locus_tag="MJE33_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBY9646520.1"
                     /translation="MRALNVVERAGNKLPDPVTIFLLLCIIVVILSAVISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDPSYQGNPAMNLYFAFISAIVLTFAGAF
                     VTEKFIAPRFTKYNDSTNDKDIQELSELENKGIKYALLSLLVVVVVIVALCIGDNAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGKVSKKIKSDKDLVSMMGSSMSDM
                     GGYIVLAFIASQFINLFNLSNLGTILSITGAKLLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEIIILLLFMGIGIPLGPGGGISYVL"
     gene            6958..8214
                     /locus_tag="MJE33_000552"
     CDS             6958..8214
                     /locus_tag="MJE33_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBY9646521.1"
                     /translation="MLKTTKISDPELYKIVADELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLETLAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSAVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECRPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIVLCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLST
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGVRIGLTSISQRGLKEKEVIQIAGIMNKVAENIDNKE
                     VLDECKAEAQELISKFPLYPEGYFED"
     gene            8386..11160
                     /locus_tag="MJE33_000553"
     CDS             8386..11160
                     /locus_tag="MJE33_000553"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBY9646522.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDNEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAHALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEDQQTPLQIKLGKLGAKIAGISGVIATLLCMFMIIQM
                     QRKGMLILDTSSVLSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITPKVEKSILDEIKKLQIKS
                     MRTLGFAYKKMSNSKTEVAVTSEGELNLIGNSRSYMKEDNLVFSGFVGIVDPLREGVK
                     DSIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFTQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRTMKN
                     SRTMNFNSKN"
     gene            complement(11261..11410)
                     /locus_tag="MJE33_000554"
     CDS             complement(11261..11410)
                     /locus_tag="MJE33_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBY9646523.1"
                     /translation="MFFISYIYTKSPIEFDTPLQKEAYKILQKLDIDFECVDTDEAIT
                     MEDCV"
     gene            11785..12963
                     /locus_tag="MJE33_000555"
     CDS             11785..12963
                     /locus_tag="MJE33_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBY9646524.1"
                     /translation="MFSKILVKTFIRDSENVQNTDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMLTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPIPVKFEVIPLILLIASIMIKIWLSRFNKFMGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEEMVNSIIELLLSYKYIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPICLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKNMSEDDLKEDITKLVKEINPEYNCVIVVDKDFI
                     "
     regulatory      13090..13175
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            13293..16211
                     /locus_tag="MJE33_000556"
     CDS             13293..16211
                     /locus_tag="MJE33_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021362860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBY9646525.1"
                     /translation="MKKGNRKALLISLIMILSMVVSTIYPTVSYASELGENSQIQSGS
                     TNSSTGEEKESDNKKPEQTPEKDKATDNKKPEQTPEEEKPTDNKKPEQTPEEDKSTDN
                     KKPEQTPEGEKPIDNKKPEQIQEEDKSTDNKKSEQALEDEKPLDNKNTEKTPEEDNLL
                     EDENLLKVLEEELNEENEDYGFVVKINNNTIETESMKKISFNLTYTPTSKGIQAGDSI
                     TFKVPDVFNKVNLDYTSECFDKTESNGEYTLTFRELPNGQSVMQGKIGLEAYVKKVDE
                     DTNAKIHIETTGKIESGSGDIDVEIKPGDKTDVPDAKGTLKKLVEGKKSTTVFMPVKN
                     KDINYSIQVNEKQEELKDIILYDELPEGLTLINGSVSVVTSDGKEVSDFNIEQSKNSI
                     SVNFGNIDKSYTVKYKARISDKNAKHGNKYKNVARIESDGKKIQEDDATVSIFDRGDD
                     YLLTKGHSGATNITQVGQVINYQISINDDKSPISNVVITDNIPEGMRLTTSGEAGHDF
                     RVVEIPMNGSWTPWSKEKIANNISYKVEEKRNESGQVDKVITGFTINLSKEEVESKFF
                     IAYTLKVISIEDSYINRAVLDANNSEIDKNDEINFKKNSGLISAKKEVDKKVLNSSDN
                     QIVKYKINMSTYGVYDAGQVNLLDEVNSVLEISNIKYSDNLELKKEAGDGKNTIRLVN
                     KYEFKQIKEGEPVQSWVTFDANFTNVKVGETIRNVAQINGSSPPGVETTKQGYAFEAK
                     KVDALDKNVLSGAKFNLEDAFGNIVVKDLVSDEDGIIQSSVKNPGTYYLVEIMAPRGY
                     EKLKDKVKVEIGNEDIGKIVDIGNIENLKQENPPVNPPIPPDTDEPIVNPPVPPSTDK
                     PRKPSSSSDTENTIVINPPVPPSEDIINPPIPEVLNPPVPPSEEMIETPVKQIIPIPE
                     VVKPSVSEEKNNKVKDDTLVNPPVPPKTGDSTTIIGEILLVIGAIVGLIVLRRNKNTN
                     "
     regulatory      16396..16481
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            16581..17243
                     /gene="zmp1"
                     /locus_tag="MJE33_000557"
     CDS             16581..17243
                     /gene="zmp1"
                     /locus_tag="MJE33_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease Zmp1"
                     /protein_id="HBY9646526.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAHADSTTIQQNKDTLSQIVVF
                     PTGNYDKNEANAMVNRLANIDGKYLNALKQNNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDSINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYPEEFFAESFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
     gene            complement(17365..18327)
                     /locus_tag="MJE33_000558"
     CDS             complement(17365..18327)
                     /locus_tag="MJE33_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBY9646527.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALSLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILNKNKLHVFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVISPILALL
                     ILKEIIPINMVIGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(18497..19687)
                     /locus_tag="MJE33_000559"
     CDS             complement(18497..19687)
                     /locus_tag="MJE33_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBY9646528.1"
                     /translation="MISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIGNPS
                     VEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTRENLIM
                     TCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIETFEPDLD
                     ALKNAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTSIYLISDEPYR
                     EIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGQMDDFDDVMSSLNVA
                     NRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKPNGTFYLF
                     PKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPAFEKLAKL
                     YNLK"
     gene            19993..20439
                     /locus_tag="MJE33_000560"
     CDS             19993..20439
                     /locus_tag="MJE33_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBY9646529.1"
                     /translation="MDFKNLQYESLSYIICNLQKNFKLYCEKCLKPYKLTNGLYFYLI
                     YINKNRNCSLNDVSTEFEVDKAHTTRTISRLEQDGYIEKIQNPNDSRAFQLRVTDKGE
                     EVLGDIKNIFSKWDNHIKKEFSDTEYKELVKNLHVVKDIKTAVEEE"
     gene            20441..21535
                     /locus_tag="MJE33_000561"
     CDS             20441..21535
                     /locus_tag="MJE33_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HBY9646530.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFSDIAKS
                     GVALITLGDVSIRPSFHKVAISLSDEDGVIKYKKIVDEIHNSGAKVSAQLFCSDYDVN
                     LIKDTMNMGITSHDEIKKIMNDGVKDYITNMPKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLVGSFSSSIFNNRNDEYGGTCDNRSRFASEIISSIRDEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLLSEVEYFVKKFESLGVNSFHVTLANHSKLEDTIPTNNHPYFKDEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMVSMSRQLLADSNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTRSQFGCILD"
     gene            21623..22153
                     /locus_tag="MJE33_000562"
     CDS             21623..22153
                     /locus_tag="MJE33_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBY9646531.1"
                     /translation="MKSLVLYSSLTGNTKKIAYTIYDEIQEEKDIKDVNELVDYGIDY
                     ENYDIVFLGYWVDKGICDKNSKQVLENIHNKKIALFGTMGASKKGSYGASIIEKIESI
                     IPKDNEILGSFICQGKIAEGLKAKYKEMLKLSPDNEHIRQQLNNHEESQSHPDEQEIY
                     EASMFAKNMMIKASIV"
     gene            complement(23088..24464)
                     /locus_tag="MJE33_000563"
     CDS             complement(23088..24464)
                     /locus_tag="MJE33_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646532.1"
                     /translation="MKYKIGFLTTSYFMSDKFKQALKELNDTCEITFIELKGENPFNN
                     LPNIYYEQVDNFDGFVCSGIVPYSELIINIKDIKVPLNFLKLDERDFYKYLFKLLNSQ
                     KDIDFTKSFMDFLREDNNYYDIYSLIDSNKCPYTIKDFNIPQPVYDFKKLNDKLLEIH
                     LDLLRDNKINLSFTRNYIISCELNKLGYDCIYMVPSTESILNTFKSLVNEITLQNLDK
                     NKSATCIVTVNSSEYINEDLIFKNDEIQNQIYNTILNSLSRHGFYGVQVKKNDMKIEI
                     HTTKEMLNNMTNNYTDFFISEDLKEIKYSLNIGWGIGNTNIHAEQNARQANGKSASLN
                     GNCTFIVNDTNDTIGPLYSNKNSNNIEDSSIANKVASFIPLSNTNVSKIMCMINDRNS
                     NNVSAEILADYMNITLRSANRILSILYKAGIATIVNTKLDNQRGRPKKIYKVDFLSFL
                     NKMQKLNS"
     gene            24733..25905
                     /locus_tag="MJE33_000564"
     CDS             24733..25905
                     /locus_tag="MJE33_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBY9646533.1"
                     /translation="MNTYLNEISKFVDENREEIVSLWKEIVNMESYTHCKESVNKLAK
                     RLKFEFEKEGLDCELVDVGDNGSTLIGTLGSNIDKKPIIFSGHMDTVFETGTFGENPF
                     KIIEGKAYGPGVLDMKGGIIISLYVIKALNKIGYKERPIKIVFSGDEEIGHKDSTGAD
                     VILREAKGGLCAFNMETGLVDNSLCVGRKGRIGCNIHVKGVETHAGNDFEGGRNAIEE
                     MANKILRIQKLTNLDVGTTVSVSVIKGGRVENSIPEDCSIKVDLRFEKVEEMENVKEQ
                     VREICKETYIEGTSTEVNFISEMMPFETTEDVMRFHTFVNEVSKENGFGELNAKRLGG
                     SSDASYLTIANVPTICSFGVRGEWNHTSREYAVVNSMFERVKLISTVILNLDKFEV"
     gene            25919..26566
                     /locus_tag="MJE33_000565"
     CDS             25919..26566
                     /locus_tag="MJE33_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646534.1"
                     /translation="MDVTAGDNKVTWKGYLSLFVLIVLFSGVFKDSQGFLKALDFANL
                     TGSFGTIADGVDFMGKGGTGAKDGFLFALTLIPTVCFAVGLIDVVESMGGMKAASKIF
                     NPLLRPLLGIPGIAGIAFVSSFTSSDVASIMTKELFESGEITDDERTIFVAYQYAGSA
                     VILNTINTQAPLLPIALLALGPIILIEIFCKILGANLVRLIIGVRNKKNKAKEAV"
     gene            26568..27077
                     /locus_tag="MJE33_000566"
     CDS             26568..27077
                     /locus_tag="MJE33_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBY9646535.1"
                     /translation="MSQQNTGNAIVNSKPKKTAVEIFMGGAKKGFYIGVEQILPAMIM
                     GYVIVQFLQLTGLVDILGNIFGPVMGIFGLPGESVVVLIAAFFAKAAGAATAANLFAQ
                     GLITAPQATILIMPCMLMGTLVGHYARIVLVANASTKYRMLLLAVPIFDAIVGMLIVR
                     VLLTVMGLM"
     gene            complement(27441..28553)
                     /locus_tag="MJE33_000567"
     CDS             complement(27441..28553)
                     /locus_tag="MJE33_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBY9646536.1"
                     /translation="MKISEVATPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLVGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKIECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     SEAASKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIEKVIEVDIRENSKQIIL"
     gene            28851..29117
                     /locus_tag="MJE33_000568"
     CDS             28851..29117
                     /locus_tag="MJE33_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HBY9646537.1"
                     /translation="MNLEPKKLIEQIGKEKDKKDIWIVGGGDIVKLFMKDNLIDDYYI
                     YILPIILGRGISLFKSGIDKTNLNFKKASNIGELVKLEYSKKQK"
     gene            29362..31089
                     /locus_tag="MJE33_000569"
     CDS             29362..31089
                     /locus_tag="MJE33_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9646538.1"
                     /translation="MKLILSYLKNYKLLIVLNILAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSILLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKDTENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSAKVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RAKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            31089..32879
                     /locus_tag="MJE33_000570"
     CDS             31089..32879
                     /locus_tag="MJE33_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9646539.1"
                     /translation="MKKSKGFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGVPGASVNFDYVTKILVILACIYFANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQSRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADKESE"
     gene            complement(33041..33349)
                     /locus_tag="MJE33_000571"
     CDS             complement(33041..33349)
                     /locus_tag="MJE33_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646540.1"
                     /translation="MFNKIYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
     gene            complement(33611..34159)
                     /locus_tag="MJE33_000572"
     CDS             complement(33611..34159)
                     /locus_tag="MJE33_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646541.1"
                     /translation="MIFLNEDVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEE
                     FGEDKTSEILRKVLFERSEERAIAMRERAHENGDELIADNIISTTDVPFLGWVPEFEE
                     LHCPYGASWLLRFEENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCE
                     RIYFHDDKVSEGKYTYGNKNCK"
     gene            complement(34262..34933)
                     /locus_tag="MJE33_000573"
     CDS             complement(34262..34933)
                     /locus_tag="MJE33_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY9646542.1"
                     /translation="MATFKRLQEQAYDYLKELILSGKMIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIVEIFQIRSAIEGFCTFLITSQYKEA
                     RAVETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDIYRTTLIHMETPKYINLEDFC
                     NNKLF"
     gene            35329..36234
                     /gene="garR"
                     /locus_tag="MJE33_000574"
     CDS             35329..36234
                     /gene="garR"
                     /locus_tag="MJE33_000574"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBY9646543.1"
                     /translation="MEGYKMKLGFIGLGIMGKPMAKNLLKDGCNLLVYDINKSAVDEL
                     ISCGAKYASVLEMGQECDIVFTILPNGTIVQDILFGIDGLAKTLKEGSIVVDMSSVTP
                     TESILCANKLKDMGLEFIDSPVSGGEPKAIDGTLAFMAGGKEEIYKKVEPFFNIMGSS
                     SILIGDNGSGSVTKLTNQVIVNLTIAAVSEAFVLAAKAGADPEKVYKAIRGGLAGSTI
                     LDAKIPMIMNRDFKPGGKISINLKDIKNVMQTAHNLDVPLPMTSQLLEIMQTLKVHGH
                     LEDDHSGIAQYFEKLAGVEIKKHDN"
     gene            36370..37752
                     /locus_tag="MJE33_000575"
     CDS             36370..37752
                     /locus_tag="MJE33_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBY9646544.1"
                     /translation="MITGYGLLIAFVISIGILLVSIIKFKVNPFLALLITSIITGFMV
                     RMPINEISTTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNS
                     ALAVTTAGFLISIPVFMDAAFVIMMPIIKYVSRVTKKSLMVFVCALGVGTIVGHALVI
                     PTPGPLAVAANVNANVGSFILYSIIVAFPAALIGGWIYGKRFEKYPAYAIDENDREKN
                     LEQGKDSIKIEDDDSSKVPGFGISMFSLLFPILLILISNVFSMFLEKGSTMSGVLAFI
                     GDKNIAILLGILVAIGFLKKYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASG
                     IGNFLVDTMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVL
                     VGLAICAGGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMVLLLSV
                     INGIIPLPGL"
     gene            37823..39259
                     /locus_tag="MJE33_000576"
     CDS             37823..39259
                     /locus_tag="MJE33_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBY9646545.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNKLLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPLETNLLKSEVERLSGKLFQGEIIMPFFKEGGRF
                     TIDNVHYVKEGEILVPAGMTEFAKDKSFGYKSSDIGEWCEEKTKGKYKSSDMIYISSK
                     ELRELNIEAITEKLKKAEGFNKIIVNATDYVDVKVFTIAFIRAVNSGKEFMFRSAAAI
                     TKVLGGVSDKELLTKDELVSKGNTNGGIILVGSHVNKTTQQLEELKNCKYPIEFIEFN
                     QHLVLQENGLKNEVKRVIKVVEEKISRGKTVAVYTRRERFDLDTNDKDKQLMISVEIS
                     DAVTSIIGMLNVRPNFIIAKGGITSSDVGTKALRVKKATVMGQIKPGIPVWMTGKESK
                     FPNMPYIIFPGNVGEISTLRESVEILMG"
     gene            complement(39445..40299)
                     /locus_tag="MJE33_000577"
     CDS             complement(39445..40299)
                     /locus_tag="MJE33_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646546.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHNIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEIYFVLDLV
                     ITVHTILQLNGKLKQINLIIKELKDKKEYYKLITQETIENKLESLVENRLDTLEDRFE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIKKDK"
     gene            complement(40567..40890)
                     /locus_tag="MJE33_000578"
     CDS             complement(40567..40890)
                     /locus_tag="MJE33_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBY9646547.1"
                     /translation="MERINCNVINCSHNNSHICYADKIFINGEKAHNDRHTCCSSFLD
                     ESIYSNLSSNVNNRNCEELVCTVKNCKYNKDGSLCTLDNILVAPETDRVNLYIETCCS
                     SFECK"
     gene            41093..41722
                     /locus_tag="MJE33_000579"
     CDS             41093..41722
                     /locus_tag="MJE33_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646548.1"
                     /translation="MFEKKKKELFPWKISALILVGVFVLSSGVYIGTRLKHNGVQTGE
                     VVSTSANMNEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMDVTIKGKYTIENDNGNIALYK
                     YDDSGSKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
     gene            42050..42559
                     /gene="ruvC"
                     /locus_tag="MJE33_000580"
     CDS             42050..42559
                     /gene="ruvC"
                     /locus_tag="MJE33_000580"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBY9646549.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKVIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
     gene            42552..43154
                     /gene="ruvA"
                     /locus_tag="MJE33_000581"
     CDS             42552..43154
                     /gene="ruvA"
                     /locus_tag="MJE33_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBY9646550.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASPAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLSESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            43261..44280
                     /gene="ruvB"
                     /locus_tag="MJE33_000582"
     CDS             43261..44280
                     /gene="ruvB"
                     /locus_tag="MJE33_000582"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBY9646551.1"
                     /translation="MQGFEDENRIITSTMKMEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSSILDAEIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            44598..45623
                     /gene="queA"
                     /locus_tag="MJE33_000583"
     CDS             44598..45623
                     /gene="queA"
                     /locus_tag="MJE33_000583"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBY9646552.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEYLNPGDCLVLNNTRVIPARLIGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFHYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLNKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKENGKNVICVGTTSCRTIESACNEYGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            45635..46120
                     /locus_tag="MJE33_000584"
     CDS             45635..46120
                     /locus_tag="MJE33_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646553.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKEQQNKEDEDKS
                     I"
     gene            46199..47320
                     /gene="tgt"
                     /locus_tag="MJE33_000585"
     CDS             46199..47320
                     /gene="tgt"
                     /locus_tag="MJE33_000585"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBY9646554.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHKFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPSDREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITNIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPKDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            47454..47747
                     /gene="yajC"
                     /locus_tag="MJE33_000586"
     CDS             47454..47747
                     /gene="yajC"
                     /locus_tag="MJE33_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBY9646555.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLSV
                     GDKVITIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            47805..48167
                     /locus_tag="MJE33_000587"
     CDS             47805..48167
                     /locus_tag="MJE33_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HBY9646556.1"
                     /translation="MGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDNITMVSS
                     FITTISAAIGGFYTSKHMKEKGLMYGLLVGLLYIVCIFLTVFLAQEKFVFEIGMIYKL
                     LLISAAGGIGGVLGVNFK"
     gene            48233..48376
                     /gene="scfA"
                     /locus_tag="MJE33_000588"
     CDS             48233..48376
                     /gene="scfA"
                     /locus_tag="MJE33_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HBY9646557.1"
                     /translation="MENKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
     gene            49003..50898
                     /locus_tag="MJE33_000589"
     CDS             49003..50898
                     /locus_tag="MJE33_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HBY9646558.1"
                     /translation="MLSNKKRSMAIVMAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAEDSDNKLTATIKSTN
                     TVEVKRAKDSANVITLNVGDQHLDFSKVITSEEGTFEGYEKSYSDIDSKELHTITVKN
                     ADLQDISAEELFDGIRLTTLGREIVNKVKNGYALTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKPETISVSSKNHKLVRDLHKVLTDVKDGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIEAEILRV
                     LGSNLSSKKIYIVGGESKVSKETEEKLSKLGVSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGGNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
     gene            51360..52763
                     /locus_tag="MJE33_000590"
     CDS             51360..52763
                     /locus_tag="MJE33_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HBY9646559.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILINSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKAKTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGF
                     TGEADAISASPVAARDGVPIILTDGNSVGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFETNKIVIQEFYKNSKEFYLSKGLQLTDALAASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     ENGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKIPDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
     regulatory      52935..53020
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            53144..59107
                     /locus_tag="MJE33_000591"
     CDS             53144..59107
                     /locus_tag="MJE33_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding adhesion protein"
                     /protein_id="HBY9646560.1"
                     /translation="MKKAISCVLAVSMCSAPLNVFAEPILEGKLRAVEKSVTEKLRGS
                     LEVDLNFSLPIKNSESESMTNMQLRLKDDSNNSGIIKLGEKDSGTVNVGTDNIEYTIK
                     KLSASRSEIKNKDENISYYNIVFNNLPVGKYNVEVSGDGFKNKEIKDIDISSYSQRVL
                     LSNRASVDNKNKPLNYELFLMGDVNGDGKVDKSDYNKVLENIDSNKREFDLNRDGKVD
                     IVDLDYVQKNLGQSEDAKSLESIVSTNPIVDTSKVELKGNSDVDINGNVENLFSGENN
                     GVTISSKNDEISEEKPAVMDIALSEALNMEVINIKAPEGTAPAKGIVEVTDKDGNVQQ
                     IPYENKEYMKNLKDEEQQEEVNNEQEEVNQEEVNNKDENINQTDNNSNNKDENNDKQQ
                     ENSDKQEANSNTNQDKNNDKENSSMDKPNSDEDTPKEEGNISKDDNSTTSTYSRSALE
                     TYADVQNSDIVINLGKQVAVKKITIKITATTANDRNLAEISKVEFLNNVYKEIPKPEM
                     NIPKINKISTSTAVGNEKVSFYWNNEVNVSAYELILDKIDSKGKVLSTKKLQTSKNTI
                     EVRDIEAYGLYRVSIQSLNGDWSSGYKDATPENLDGVPENVDSNYKPIQFSPDSILEF
                     QVVPDTKPEPPEGINVKGKFKSLDVSWKNHKQAKDFDLYYKEKGSNGSWIKYNDKLIR
                     GTSSKIEGLKDNTTYEVRMTATNHLGTSGMSKTYIGTTESMDAPTTPNYRLINTPKEN
                     GGFGIPTNHIVDVVYPDGYDKSEYSDKNPFDKFNVVDGDFTTHWTFPTYNAAEGTNRG
                     PIITFDKSYTMDTFMATPRFDVSDKTINDFEVRYWDENGQLHNLGDVPFETKTDPVNN
                     NKYVMVRLKEPITATKIQVSVCSSYSYKSNISELKFYEYDSIEDDVRNLFMDGLQVEL
                     KEDVTQEKITELKDRLNTPDKASGEYHPYKTVIERELKLAQDLYNDRATLTDVTTVNQ
                     EINKLGKYKDKNGQYTVNSNNLGMQNDWQALGVAARAGDEITVYVGSKSGKTPKLIYT
                     QYYGESGAYKSGEINLNTGKNVIQLSKLHSLDIECGGSLYIRYAEDTPSGGDIKVRVA
                     GATKIPHLNLNGTINDKSPAGVSESKKKIKAYIEELAKYKDAVKGEPYYPGTNVGNSY
                     GYSEKTGVLNTTDIESDKVTLNVPATAVYQEISKGNSDLNTQVDRLYNSMLAWEQIMD
                     LVYSERGVFKTQDLDGNGTVDDNEFNLTKNDMAPKSRMNIKYQRMFIGAFMYASGLHV
                     GVEYGSVPGLMNGVPFQIDDSGKATGGNLFGWGIGHEIGHVTDIGKMTYSETSNNVLA
                     LLAQTFDDKTHSRLEGSTMDKIYEHVTSNSLGIPSNVFERLGMLWQLHLAYDDDFTGS
                     MLKNNSDADLSNDTFYAKISRKYRALSSSDPINSLPKDQMLVAMASSVVEKDLRDYFK
                     AWGVEITPELNSIMDSKNYEKESRKIQYVNDEARRKILNENIASMAKDTKVSASFSDG
                     IQSGSLVKSNKISIDLDVDKDKDKILGYEIIRSDGNYLDGENGTQVKYRTVGFVDAKG
                     NSKTKFTDNISPLNNRAFTYKVVAYDYHLNPTEEFEVGTVKLSDEGKINKSAWSFITN
                     TVSDGDVRTENDSHGPLQNPEIDNIKDNDASTTYKGKIISKAEWNKDPQKDPDINVEE
                     NPYIIVDMKETLPIVGLKYTKPETEARKFSLKGLFNFRKNTSTTYNPLTNYKIQVSND
                     KKNWEDVSQGTFEYGQDLVGGGKDQDNEARVTFNKDGKLWTYQARYVKLISNNKSNIE
                     VAELDIIGPPGDNIEIGIINNADETRTNGIGKLAEDYVYQSDDSSTSEDETGKIPAGS
                     IVITGEYSGNPAFNLPLLIDKNNKTISGEALLFAEVPEKGELGEISKGTWVYYMPKEN
                     FDSLSDKVKAELYRYNDLQGDTPVGQRFVSDTMYVPVGAKNYDELKTISLTDSNSKAR
                     KATRNSIDISNKKVITVSNKVKGNIVRDK"
     gene            59311..61191
                     /locus_tag="MJE33_000592"
     CDS             59311..61191
                     /locus_tag="MJE33_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp10"
                     /protein_id="HBY9646561.1"
                     /translation="MSKRKSFIRTIAVSTMAVAVTGSATCAYAAPVLQGTKTYEKVNT
                     IDISVDSVENIVYSFQASIKVQGEVEVVDNEQKEKITWSDNIKSQIKSGNANATCRVE
                     YNKSSNTTTLDIYVTSNEDLLDGNRLNIGRISVKKSGSNSNADYKVLGKGTNDKPALK
                     IVTYNNKTVDYENISSDEGLIFTLINESEVKPIGGTGSSKNDPEKYKVEKSEALEYLL
                     NNIRINYSIVSKETQESGSNVILKLGLAQKTTKGRKATINKYVEVTLPKSLEYIVENE
                     LSKPDELPPDNGNGGNNGGGSNSGGSSSGGSSGGGSSSDSTSNVTVKKLKGADRFETA
                     IKISQSGWTKSDTVVIVNGEDKSMVDGLTATPLASVKNSPILLSSNEKLPQKTVEELK
                     RLNPSKVIVIGGNNSMPNSVVEAIKAVNSKISVQRIGGDTRYQTSINIAKEIDKTNNV
                     SKLYIGAGNGEADSLSIASLAGKEKTPIVLTQKDGVDNEAEQFIKSNKVSNIYFIGGV
                     EKISNKAIEQVGKIANKDISNNRVAGQTRQETNAKVIDKFYSQSKLDGVVVANQDKLI
                     DALAVGPLAAKNNSPVILATNTLDKSQESSLKGKNSSKLFEVGGGIASSVIDKIKSLI
                     EK"
     gene            61558..63159
                     /locus_tag="MJE33_000593"
     CDS             61558..63159
                     /locus_tag="MJE33_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HBY9646562.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADKQTYETVILVNTEKSLADGLSASGLSGATKAPILFTQQNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELDSKNIEVKRIGGEDRLKTSYLIAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKSVPFDVKNIQSYCIGSEEIMSNPLVKNTNSVR
                     IEGTDRFETNKNVIDYFFNSADGFYVSDGYQLVDAIAAAPLTKNSPMVLVNDGSDKTV
                     LEGAKNITSVGEINEKVIQQCINASNSNGQPPTITVGSTEVYKGEKFDTGKLNIVAKD
                     NTGKVLPIEVDGFIDTNRVGTYILTLKATDEWGKSTGKRVEIKVLDDKSHDYNSPEFK
                     KMVSTEMYNLINSYRKEKGKEPLLVSSRLEGMANAWSKYMMDKKVFAHYIDGKNAPQV
                     FSEFGMRSEENIAYIYIDSKNVQTTQDAKDLAKAIFEVWKKSPEYNANMLSNEFYSTG
                     FGLYILSDGQVHATQEFLNGNEGSL"
     gene            63378..64976
                     /locus_tag="MJE33_000594"
     CDS             63378..64976
                     /locus_tag="MJE33_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBY9646563.1"
                     /translation="MKVNKRVLSIGLTISLIMAGAPNINALSSIEKIQGKDRYETSAL
                     IADKQIYDTIILVNTDNSIVDGLSASGLSGVAKAPIMLVQRDKIPTDVEKRLKDVKNA
                     YVIGTEDTIGKSVENQLKNKGIEVKRIGGEDRIKTSYLIAKEISAIKPVNDGDKVFLV
                     NGYTGEADAMSVSSVAARDGVPVILTDGKSIPFKVDGVQCYSLGSEEIMSNELVSKTN
                     SVRIAGKDRFETNKKVIQRFYKGTKKFYVSQGYKLVDAVAGSPLAKDKPIVLVNDGSD
                     KSVLRGADEVTSLGGMDKKVVDQCISSASDKNTMPTITANDVEISVGDKFDNSMLNIV
                     ATDYYGNDLKANIKGNVDINKAGTYVLNISVVDNLGQKSEISVNVKVVVNASTKDSNS
                     YEFKAMVSNEMYDLVNSYRKEKGKKSLRELDSLAGMANAWSKYMEDKKVFAHEIDGKN
                     AAEVFFGFGARSGENIAYLPMNVKSVYTSKDAKEMAESIFDLWKKSSKYNENMLKEEF
                     YSFGFGMHVSSKGEVNATMEFLNS"
     gene            65266..67464
                     /gene="slpA"
                     /locus_tag="MJE33_000595"
     CDS             65266..67464
                     /gene="slpA"
                     /locus_tag="MJE33_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HBY9646564.1"
                     /translation="MNKKNLAMAMAAVTVVGSAAPIFADTTVKEEGYTVVQDKYEKLL
                     KELKAKIKDGTITSVGVEFDGKPITTLAPKADGSDKDAIAEQLETLTKNQLKGLGDGK
                     YVDFKITYGAKAEVPAASLSADDIQKYADQINASEKILVEVAAGSEAGIAKFDSVNNK
                     VIAGDAPLKVKDAVKATVTTNGSNKKVLTISAAAGLSGFSYGTLKDTGASLSDVDAIT
                     LDTTNATITEGDTKVLDFDNSFKFNESTKKVGSLVTPNTTNTPADPGTKTTVRVIKAV
                     EKTIDVSSNSTTKAKDLAKQYVFTDVSDTDPESLSYMLKNINDGKVAVKNSDGDYEVT
                     IFPEGKRLNTLSASSAKTILGDKDTPAKIVLKASTTKKLADYIDDLITYNNSYSNVQT
                     VAGSDRIETAIELSRKYYNSTDKNALYGDPVNNVVLVGSQAIVDGLVASPLAAEKDAP
                     LLLSSKDKLDSSTRAEIKRVMDLNSSTGIKNNKEVFIAGGVNSISKDVENELKDMGLK
                     VTRLSGDDRYETSLAIADEIDINDKAYVVGGTGLADAMSIAPVASQIKDGEATPIVVV
                     DGKSDKLSKEAEDFLDDAQVDIIGGENSVSAKMEDYIDDATGKSPERISGADRQATNA
                     EVIKEYFDKDGVSNYFLAKDGSTKEDQLVDALAAAAVAGNYGSKHNEDGDITTDASPA
                     PIILATDNLSAEQHVAVSKTATTNGAKNLVQVGQGIADSVVSKLKDLLDM"
     gene            67710..70052
                     /gene="secA"
                     /locus_tag="MJE33_000596"
     CDS             67710..70052
                     /gene="secA"
                     /locus_tag="MJE33_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBY9646565.1"
                     /translation="MSVLDVILDKTDELEIKKLNNIVDKIDALEDKIQILDDEALKNM
                     TNVFKDKLSKGETLDDILPEAFAVVREVSKRILGMRQYRVQLIGGIVLHQGKIAEMRT
                     GEGKTLVGVAPVYLNALTGKGVHVITVNDYLAQRDKELMQPVYEFLGLTVGVIINGQQ
                     PVERKLQYQCDITYGTNNEYGFDYLRDNMVTQKSEKVQRELNFAIVDEVDSILIDEAR
                     TPLIIAGGGDEGTKLYELANAFIKNIRDEDFEMDRKDKTIALTASGLSKAEKFFGIEN
                     ITHIDNIEIFHHVNQSLRAHKLMNIDVEYVVRDNEILIVDEFTGRIMDGRRFTDGLHQ
                     AIEAKEGVKINDESKTMATVTFQNFFRLYSKLSGMTGTAKTEESEFEATYKINVVQIP
                     TNKPVIRADLHDKVFKTERAKYNAVVEEIIRINKTKQPVLVGTVSIEKSEKLSKLLKK
                     KGVKHEVLNAKQHDKEAEIISKAGKLGSVTIATNMAGRGTDISLGAGDKEEEEKVKEL
                     GGLYVIGTERHETRRIDNQLRGRSGRQGDPGTSRFFVSLEDEVIKLYGGKSIEKILKR
                     MNANEDDALESKALNRAIERAQKGIEGKNFEQRKNVLKYDDTINEQRKVIYDERNKVL
                     DGSDIEEEIQKMVMDIITEAAEKYLKKFRDYHGYFKYLYNTFMPADTLLIPDLDKKNV
                     EEIVNQTYQISKRVYDLKKMMIGIEELAQLEKRVLLQVVDTYWVDHIDAMDQLRQYIG
                     LKSYAQKDPFKEYAIEGYEMFEALNRNIRITTVQYLYKFN"
     gene            70466..72337
                     /locus_tag="MJE33_000597"
     CDS             70466..72337
                     /locus_tag="MJE33_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBY9646566.1"
                     /translation="MNRKNLYVIMAAAMVSTSVAPSFAAEATQVKKQTITKKEATELV
                     SKVRELMAQRYTGGSQVGQPIYEIKVGETSSQLKIITNIDELEKLVNALGENKELLVT
                     IADKGHTTNSANEVVAEAIEKYENSADLSAEANSITEKAKTEATGIYGVATVEASYDS
                     TKDKLVITLKDKTDVVTSKTLQIGIGDEKVDLTANPVDIAGTNLDPSAEGFRVSKINK
                     LGVEGAKNIADIQLAEITIKNSDLNTVSPQDLYDGYRLTIRGNTIANSVGKSISDISS
                     KDSETGKYKFTVKYTDTSGKAIELTVESTNDKELKDVKDALNGNTKVNLIAGEDRYAT
                     AVAIAKETEYTDNIVIVNSNKLVDGLAATPLAQSKKAPILLASDNEIPKVTLDYIKEI
                     IKKSPSAKIYIVGGESAVSNTARKQLESVTKNVERLAGDDRHTTSVAVAKAMGSFKEA
                     FVVGAKGEADAMSIAAKAAELKAPIIVNGWNDLTADAIKLMDGKEIGIVGGSNNVPSQ
                     IENQLADIDKDRKVQRVEGETRHDTNAKVIETYYGKLDKLYIAKDGYGNNGMLVDALA
                     AGPLAAGKGPILLAKNDITNSQKNTLDKKLNLGAEVTQIGNGVELTVVQKIGKILGW"
     gene            72446..73153
                     /locus_tag="MJE33_000598"
     CDS             72446..73153
                     /locus_tag="MJE33_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBY9646567.1"
                     /translation="MIEATLFFSPHQDDETLSMGSAIIEHVERSDTHVILCTDGSKSI
                     IRKVLDDGGSCSYHIKDVHKYSLSESDFSKNRDEEFKNSCKAMGVKENNIHIENNRAH
                     DGELSKEKARDIMLKYLEKYPDAKVKTVTPYKALGIHEDHRALGEAALELYREGKIKD
                     LRFYVEPYDYNDFKKVNPNVRVWKVLSSQEEKLLNAMDAYKKWNPENGNYAIGYHSVK
                     SHFDELKSNKIQYVHAP"
     gene            73186..75318
                     /locus_tag="MJE33_000599"
     CDS             73186..75318
                     /locus_tag="MJE33_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBY9646568.1"
                     /translation="MKISKRIMSLLTATFFIFALSINIADASSKESLVGSGRWETAIK
                     ISQNGWKDATNAILVNDNSIADALSATPFAKLKDAPILLTQSDKLDSRTKAELKRLGV
                     KNVYLIGGMNTLSTNIEKQLKSENMTFERISGNSRYETSLKLAERLDKEKSISDIVVV
                     NGEKGLADAVSVGSAAAQENMPIILSDPNNGIKVANSFISSKNIKKSYIIGGTYSVSS
                     SIEQSLPNVKRLSGNNRNETNAKVLEEFYKNTDLKNAYITKDGMKKQGDLIDSLAVGV
                     LAAKNSSPVVLVGNSLDSSQYDVLNTKSFDNIVQVGGNGNEDAVKDILDLQEETIYRV
                     KTADELDVALRKADANDIITFKPDDNKKITSSFSIETKKAITVELEGLYTQTITVNMP
                     NGELTNKGTLDGTVKIMDIKNGTFINEGDINDIDIYDPNGCKIENKSGGDIWVLTISS
                     SAKDVNIENKGDISKVTNNSTSVIIRNSGNIDTLSGINAPAISGNKPRVDDTDKDEKR
                     ASGIYPTSEPLSPARAGYISVKIFTKPEDTNYKIYYRVVSSRPSALYIGDRIDTSDWT
                     AVPSSVLPFTIKAENGYYIEAVEINSDTKKVSRWGKSDYIDDGFYLKSETAKGLSADI
                     DFDGDYVKLTATGADSGCTVYYRIVSKKPVSMNVGDKVTISDWDEITTNRVRIEANDV
                     KDKYIELVELDDSTNKVTRWGASDKVVE"
     gene            complement(75371..75766)
                     /locus_tag="MJE33_000600"
     CDS             complement(75371..75766)
                     /locus_tag="MJE33_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBY9646569.1"
                     /translation="MILKKHRETILYLFFGAFTTLVNIVSYLFFTRVILFNFMVANAL
                     AWILAVLFAYVTNKFFVFESKRIELIFLFKEFLFFVSFRLLSGIIEMIIMYIMIDLFF
                     VNDITVKVFTNIIVIVLNYLFSKMIIFRK"
     gene            75993..78404
                     /locus_tag="MJE33_000601"
     CDS             75993..78404
                     /locus_tag="MJE33_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp84"
                     /protein_id="HBY9646570.1"
                     /translation="MRKYKSKNLSKLLALLTVCFLIVSTIPVSAENHKTLDGVETAEY
                     SESYLQYLEDVKNGDTAKYNGVIPFPHEMEGTTLRNKGRSSLPSAYKSSVAYNPMNLG
                     LTTPAKNQGSLNTCWSFSGMSTLEAYLKLKGYGTYDLSEEHLRWWATGGKYGWNLDDM
                     SGSSNVTAIGYLTAWAGPKLEKDIPYNLKSEAQGATKPSNMDTAPTQFNVTDVVRLNK
                     DKETVKNAIMQYGSVTSGYAHYSTYFNKDETAYNCTNKRAPLNHAVAIVGWDDNYSKD
                     NFASDVKPESNGAWLVKSSWGEFNSMKGFFWISYEDKTLLTDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETN
                     IDAYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGESLT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKAELQRLGTKKVYLIGGENSLSKNVQTQLSNMGISVERISGSDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISVGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKSQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKNVLKSKKIETPIRVGGNGNENAFNELN
                     TLLGK"
BASE COUNT        31153 a         8997 c        13831 g        24625 t
ORIGIN      
        1 ataaaagtga tacaataata atagaagtaa taaaaaaaaa taattaatat aaaaatgtgt
       61 acaaactaat gaatattgat atagtaagct atcatatgaa agagtatgta cacatttttt
      121 ttactaattt ttcacaatat gaacataaat aatttacagg aaaggaatct tttatgaaaa
      181 ttcaaaatac tgagtgggga tatattgaat ggaaacatac ttatgatgaa aataatccaa
      241 aacaagctat gaatatatac attgcagtaa ctatgcctgg taagaaacat tttaatcatg
      301 ttcattatgg tcaagagcag atgatatata ttttagaggg agagggtttg tatattataa
      361 atggagtttg gaagccattt tatcaaggta tgatttttta tatagaatct ggtagcacac
      421 atgaaactat aaatacaggt gatagagaaa taaaagaact tattgtttca aataatgtag
      481 atgatgtagg agagagtgaa gttatagata ttaatcctaa taattattta aagaagacct
      541 tgataaatta tagtgaaagt acattaaatt tgtatgcagc agtagaatca attcgtggtc
      601 aatttataga cccgtttaaa attccattaa ttatatatga tgattcatgg aatatagttt
      661 taaaaaatcc ttattttcca cttttttgtt ttgaaaaatg taatcctatg aaatttcctc
      721 aaaattgtga ttgtatgaat caaaaaagta gcaatcaatt tgtatgtgaa tatgggataa
      781 ctatatataa tataccaata ctgtacaaaa gcaatagcat aggtgttata agaggagggt
      841 atgtactatt atctgatttg aatttagata cagagcataa taatttatat gatattccag
      901 agggagctgc tagaagtata aaacgcctcc tgaaacaaat atctaaaaat ataataaatt
      961 tttgctcttt taatgatata agaaaggatt tacaagaaaa agagaagact atagcaagaa
     1021 catatcatta tggagagcaa cttgaaatga atttaaaagt agctcaagat atggttacta
     1081 atttaagaat aaatcatcac tttttattta atacattaaa ttcaatggca agcatagcgc
     1141 tagatgatgg tagttatgat ttgtatagtg caataataga tttatcaaga atgtttagat
     1201 atacaatgag gtctgattta agatttgtag aattagaatc tgaaatatta tacattaaaa
     1261 attatttgaa tttacagaaa ttacgttatg gagatgcact taaagtaaaa tatcttattc
     1321 cagagaagtt atataacctt tctgtgcctt ttaactttat tcaacctatt gtagagaatg
     1381 catttacaca tggatttaga gatattgaca cagaaaagcg aattgaaata attgcaagat
     1441 tagattctca gtatgctatt atagagattc ataataatgg aactatctta gatggaaata
     1501 atatagacaa aataaaggca ggtattcgta gtaataatgg tcatggtttg tctttaatat
     1561 atacaaaatt cacatctgct tatggaaata attttgatat ggatataaag tcgagtgata
     1621 atgaaggaac ttacataatg attaaaattc ctattgaaaa ttataaggaa tatgtataga
     1681 acttgtgaaa aattaataag ttttataaat gttaagaaat atattagatg tttttatgtt
     1741 gttttatata tcttactttt ttatattatt aagctaaaag ggaggtattc tcatgagaag
     1801 agcactaata atagatgatg aagaagctgt tctaaaaata ataacacatt ttatagaaat
     1861 gaaagatatg ccaataaaaa ttgttggtaa agcaacatct ggtgatgaag cagtagataa
     1921 aatcattggt ttagagccag acatagtttt tatagatatt caaatgccaa tatacaacgg
     1981 gttagaagta atagaaaaaa catctcattt atctaaaaaa ataaactttg tagtaataac
     2041 tgcatttaac tattttgaat atgctcaaaa agctttgaga ttaaatgtaa aagatatact
     2101 ccttaaacca ttggatatga aagaatttac taagtctgta gaaaaaatta ttggatatca
     2161 atataccaat aatgatttat taaatgagat actggaatat ataaatctaa actatagcaa
     2221 tgacttaaag ttaaatgatt gtgctagatt atttttaacc aatccatcta atatatctag
     2281 gatttttaaa agtaatttaa atacaacttt tatatcatat ctaaatcata taagaataga
     2341 aaaatctatt gagttacttg aaaatactac aaaaagtata aatgagattg cagaaatagt
     2401 tggatataat agcctaaata acttttataa aaactttaag atagaaaaag gaatgactcc
     2461 aaaagtttat aaactgaaca ctaaaaattg aaattgataa ataaaaagtc aatatagata
     2521 attgtttgag tgtgtctgaa taagtatact ttaattatga taaaaattaa agcgctttaa
     2581 acatatttaa taatattaaa ccttataaaa gctgtaccta tttatataaa aaaatatata
     2641 taaataaaaa gaaaaagata tacaaaataa aaaaagtgaa ttaaatactg ttttaaaaat
     2701 acagaatttt catttaattc actgagagtt tatatttctt aaaggaaggt aactttataa
     2761 tggaggtaat ttaatatgtc agacaaagtg aatatgaaaa atattatctg tattgcaggc
     2821 gcttatctag caacagcaat tggttctggg tttgctactg gtcaagaaat tctacaattt
     2881 tttgcctcac aagggtatat gggtttaatt ggagcattaa tatcaatggt attgttttca
     2941 tttcttggtg ctgaagttat aactagaggt agagaattaa aacttaaaga accaacaaga
     3001 atttacactt tttattgtgg gaagatttta ggaaaatttt acgaatggtt tagtccaata
     3061 tttttatttg ggattcttat agtaatgatt tcaggtgcag gctcaacttt aactgaatac
     3121 tatggtataa atccttactt aggaagagta ttgatggctg ttgctacttt aattacagtg
     3181 tctatgggtt tagaaaaatt agtaagcata cttggacgta ttggaccagt gatagcagta
     3241 tttacaattg ctgttggatt aataagcttg tttaagcatt ttgacggttt atctatggtt
     3301 ggagaagcta tgaaaaatat tgaagttaca aaagcatcag aaagtccaat cgtttctggt
     3361 gtaatatata atacattaaa tattattgta atgattgttt ttttaacagg aatgggagct
     3421 tctattgaaa gtaaaaagga tgctttttgg ggaggtttat taggtggaat agtgtttatg
     3481 actgctggta tagttatgta tttggctatg atttcagata taggaaattt gtatatgaaa
     3541 gaaataccat cactttattt agcagataat atatctccga ttgtaggtgt atgtttttca
     3601 gttgcattaa tattaggtat ctatacaaca gctgttccac ttttatggtc tgtaaccaat
     3661 agaattgttg aagatgaaca tcctaaattt aaattggtaa caacagtaat agctattttg
     3721 gcttgtatag gaggtttttt accgtttggt aaattagtaa acattttata tccatataca
     3781 ggttatatgg ggcttttaat tttagtctgt atggtgtata aagcaattac atgtagaaaa
     3841 aaatcaacac aaagtcagga atagagccac ttattaatag tttttattta aagaaatttt
     3901 ttatataatc cgaatttaaa aagtcaaaat agttaaataa attgcaaaac atgataatat
     3961 atttaattaa aataatatag taaaatgtaa atataaacta aaattaaatt tataaaaaat
     4021 atctttataa agattattaa cttatataat ttgtagttaa attataaaaa ataaaaaaat
     4081 aaaattttgg agaggtgtta gtatgcatga acttatttta atctcagaaa agtataaaga
     4141 agatatttta gattttttta cagatattca tatgcacccc gaattaagtt ttaaagaatt
     4201 taggactaca aaagctataa aagatttatt agtaagttta gacatagaaa tattagattt
     4261 aggaatggaa acaggagttg ttggattatt aaaagggaag tatgatggtc ccacagtagc
     4321 actaagagga gatattgatg cattgccaat atatgaagag gttgatatta agtataaatc
     4381 cagatatgat ggtataatgc atgcatgtgg gcatgatata cacacgtctt gccttgtagg
     4441 atgtgcatat gtattgtctc atattagaga tagtcttcat ggaaatgtaa agtttatatt
     4501 tcaaccagcc gaagaagtca ataaaggtgc gaagatgcta gtggaacgag gagtaatgga
     4561 aaatccaaaa gttgatgcta tatttggact tcataatcat ccagatatac cttgtggaaa
     4621 aataggtgtt aagttaggtg ggttaatggc agcagtagac actattaaga tagaagtaaa
     4681 tggatttgga ggtcacggag gtataccaaa cagaacaata gaccctatag tagcatcaag
     4741 tgcaattata atgggtattc aaaccatagt aagcagaaac ataagtccat tagaatcagc
     4801 tgttatatca ataggtacta taaatggagg gactgcaaat aatgtaattt cagaaaaggt
     4861 agatatgaca ggcacatgta gaagctttag taatgaagta cgaaagaaaa tatcagaaaa
     4921 tttagaaaat atagtttgtg agatagctcg tggatatcaa gcaactgcaa agctagatta
     4981 tttatttgat ttaccagctg ttataaatag taaagagatg tacgcaatag cttgtaagtc
     5041 tgtgtgtgat ttgtattcag aagatgctat agttgatcca ataccttcta caggaggaga
     5101 agatttttct atattcatgg aaaaagcccc aggatttttt tactggttag gggtaggcaa
     5161 caaagaacaa gattgtatat atcagtggca taatcctaaa tttaaagctg ataaaaattc
     5221 tatattggta ggtacaaatg tactatgtca gtctgttata aattatatgg ataaattaaa
     5281 aaataaaatt taagataaag cattctggag ataaagttta tgagggggta ttagtttgag
     5341 gaaaattaat tcacaggaaa aaaacaaaga tagtggatta ttgatgaggg cactaaatgt
     5401 tgtagagaga gcaggaaata aacttccaga cccagtaacc atatttttat tactatgtat
     5461 aattgttgta atattgtcag cagttatatc taatttagga gtagaagaaa tacatccttc
     5521 tacaaaagaa gttgtaaagg tagtaaatct actagagaaa gagcagattc aatcatattt
     5581 aggtagcata gttacaaact tccagtcatt tgctccttta ggattagtac tggttacaat
     5641 gcttggagca ggtgttgctg agaaatctgg atttatggaa gtgttaatga aaaagggtat
     5701 aagtaaagtt ccacaaaaac ttgtaactgt agcaatcgta tttgcaggta tgttatcaca
     5761 tacagcagca gatgtaggat ttataatatt accaccactt gcagcacttg tgtttttagg
     5821 aattggaaga catcctcttg taggtatgtt tgcagctttt gcaggtgttg cagggggatt
     5881 tgcagcaaat gttatgctta gtactacaga tgtattgtta gctggattta caataccagc
     5941 agctcaaatg atggacccat cttaccaagg caatccagca atgaatcttt attttgcttt
     6001 tatatcagcg atagttttaa cttttgcagg agcatttgta actgaaaaat ttatagctcc
     6061 tcgttttaca aaatataatg attcaacaaa tgataaggat atacaggaat tgagtgaact
     6121 tgaaaataag ggtataaaat acgctttatt atcattgtta gttgtagtag tagtgatagt
     6181 tgccttatgt ataggtgaca atgcatttat gaaagatcca gagactggtt ctatactatc
     6241 tagtaatgca cctttaatga aaggtatagt tcctataatc actattatat ttttaactcc
     6301 aggattagtg tatggaaaag taagtaagaa gataaaaagt gacaaagatt tagtatctat
     6361 gatgggtagt agtatgagtg atatgggtgg atatatagtc ctagcattta tagcatccca
     6421 gtttattaat ttatttaatc ttagtaactt aggaacaatt ttatcaataa caggagcaaa
     6481 attactagca gagtcgggaa tacctagcta tggattgata ataggattta tattgctatc
     6541 tggatttatc aatttatttg taggaagtgc ttctgcaaaa tgggcgattt tagcacctat
     6601 atttgttccc atgtttatgc tactagactt taatccagca ttaactcaaa tagcttatcg
     6661 tattggagat gcttcaacaa atccaataag tccacttttt ccgtattttc cagttatatt
     6721 ggcctttgcc agaagatatg ataaagatat aggtataggt actgtcatat cgaatatgat
     6781 tccatactca gtggtattta cattaataga aataataata ttgttactat tcatgggtat
     6841 tggcatacca ttaggacctg gtggaggaat atcttacgtt ttatagacca ttataataaa
     6901 tttatcaatg aagattaaac taataaaata tattctttaa ttaagaggag gattattatg
     6961 ttaaaaacaa caaaaatttc agacccagag ttatacaaga ttgtagctga tgagttggtt
     7021 agacaagaac acaatattga gatgatagct tctgaaagta cagcaccaac agaagtactt
     7081 gaattaagtg gatgtgtatt tacaaataaa acagaagaag gctatccagg ggctcgtttt
     7141 caagcaggtt ctgaagaagc agataaactt gaaaccctag caataaaacg tgcaaaagaa
     7201 gtatttggag cagaacatgt aaacgtacaa ccgtattcag gttctacagc taactattgt
     7261 gtatattcat caatattaaa accaaatgac actgttttaa gtatgcgtct tgaccaaggt
     7321 ggacacttga cacatggtag cgcagttaat tttttacatg acatttataa atatgaattt
     7381 tatggggtag acccaaatac tggacgtata gattatgatg ctcttgaagc aaaggcaaaa
     7441 gaatgtagac caaaacttat aattgcaggg gcaagttcat atccaagatt aatagattat
     7501 gaaagaatat caaaggttgc aaaagaagta ggagcttact ttatggtaga tatggcacat
     7561 gttgcaggat tagtcgcagc gaaagtaata cctagtcctg taccttatgc agattttgtt
     7621 tcttcatcta cgacaaagac tttctgtgga ccaagaagtg gtattgtact ttgtaaagca
     7681 gaacatgcaa agaaactcga taaaggtgtt tttccaggaa cattagggtc tatacatctt
     7741 aatacagttg cagcaaaagc cttttcacta ttatatttaa gtacagataa atttaaaaag
     7801 ataatggagc aagttgtagt aaatgctcag actctagctt ctgaactgat ttcacatggt
     7861 tttagtattg taagtggagg tactgataat catatagtaa tggttgactt aagaagtaag
     7921 aatcttacag gaaaacaatt tgaaaaagct cttgaatatg taggaataac agttaataaa
     7981 aatgttatac ctgacgaccc acaatcacca tttgtaacta gtggtgttcg tataggtctt
     8041 acatcaatat ctcaaagagg tctgaaagaa aaagaagtta tacagatagc agggataatg
     8101 aataaggtag cagaaaatat tgataataaa gaagtattag atgaatgtaa agcagaggca
     8161 caagaactta tatctaagtt tccattatat ccagaagggt attttgaaga ttaaatattt
     8221 tgatttgtat agctcacaat ttataaagcc tgtaacataa aatttaataa tatataacca
     8281 ctataaggtg ttgattaatc aacaccttat tttttttgaa aagtaatata caagctatca
     8341 taagcatata attccttata gataattaac aagggggata aagttatgag gtattataat
     8401 aaacctacca aagaagtatt aaaatactta aaaacaaatc cagaaattgg acttgatgat
     8461 aatgaggtag aagaaaggaa gcttagatat ggattaaatg aatttactat aaaagaaggt
     8521 agaacatttt gggacgagtt gggagaaagt ttgacagaac ctatgatatt aattctcata
     8581 ggagcagcag taataagttc ttttgtggga gaattacatg acgcattagg tattttaggg
     8641 gctattttta ttggaatttc tataggcata ataactgaag gaaaatctaa aaaagcagct
     8701 catgccctgt caaaattaac agaaaatata gaagtaaagg tgcttagaaa tgggaagata
     8761 attaaaatat caaaaaatga tttagtacca ggagatattg tatatataga aactggggat
     8821 atgataccag cagatggaag acttatacag tctataaatt tgaaacttag agaagatatg
     8881 ctaacaggag aatctgatga tgtagctaag aatgctgatg ctgtattaga tatggaagta
     8941 gtttattcta aaacagaaat aatcgaacag gatgcaatac ctgcaaagca agtcaatatg
     9001 gtatttggag ggactcttgt agcatatggt aggggcataa tggttgtcac tcatactggt
     9061 gataagacag agatgggtaa gatagcacag aatttatcaa atgaagacca gcaaactcca
     9121 cttcaaatta aattagggaa gttgggtgct aaaatagcag gaatatcagg cgtaatagct
     9181 actttattat gtatgtttat gattatacag atgcagagaa aaggtatgtt aattttagat
     9241 acaagcagtg tattatcttt cttacaatca ttagaacctg ctaaaaatgc atatatggtt
     9301 tgtattgcat taatagttgc aactgttcca gaaggtttgc caactatgat aaatattacc
     9361 ttagctataa caatgcaaaa gatggcaaag ataaatgcat tagtaacaaa gaaagaagcc
     9421 tgtgaaacta taggttctgt ttctgtaata tgttccgaca aaacagggac tttaacacaa
     9481 aacaaaatga tggtagaggt agcatatgtt gatggtaaat atataagtgg aggagagtat
     9541 caaagtaata gctattttga acaaaactgt atagtaaatt caactgcaga tatagaaaaa
     9601 gaagataatt catttaaata tataggaagc gcaacagagt gtgcattact tttatatcat
     9661 aatgataaga actataatga aatgagaaaa caaacgtatt tgatatcaca aataccattt
     9721 agttcagaag aaaagaagat gtcaacatta attcgtcaag aggattcaga tatattactt
     9781 tcaaaaggtg ctccagaagt attattaaaa aaatgttctt atgtacaaca aggaaaaaat
     9841 attgttccaa taactccaaa agtagaaaaa agtattttag atgagattaa gaagttacaa
     9901 attaaatcta tgagaacatt aggatttgct tataaaaaaa tgagtaattc taaaacagaa
     9961 gttgcagtga cttcagaagg tgaattgaat ttaataggaa attcaagaag ttatatgaag
    10021 gaagataatt tagtttttag tggatttgta ggaatagtgg acccattgag agaaggggtc
    10081 aaagattcca ttgataaagc atttaatgca ggcgtagatg taaaaatgct tacaggagat
    10141 aatataaata cagcaacagc tattggaaat gaattaggat tattaaatga tgggaaaaag
    10201 gcagttgaag caacatatat agatgtattg acagataaag aattgagaga agaaatcaaa
    10261 ggaatatcta ttgttgcaag aagtaaacca gatacaaaaa tgagaatagt ttcagcgctt
    10321 caaaaaagtg gcgaagttgt agctgtaaca ggtgatggta taaatgatgc cccagcactt
    10381 agtcaagcag atgttggaat tgcaatgggt atttcaggaa cagaagtatc taaaaatgca
    10441 gcagatataa tattgactga tgatagcttt agtactatag tagagggaat aaaatgggga
    10501 agaggtatat atgagaactt tcaaagattt atacagtttc agcttactgt aaatatagta
    10561 gcatttataa ttgctataat atctcaactt actggtaagg atatgccatt tactacaata
    10621 caattattat gggtaaatat aataatggat ggtcctccag ccctagcatt aggattagaa
    10681 cctgttagag attatgtatt gaaaagaaaa ccaataaata gacattctgg aataatagct
    10741 aggtctatgt ttgtaaatat tattataaat gctattctta taattacaat tgtatttact
    10801 caatcagctt ttaatatatt gggagctaca tcagaagaac aaggtactgt tatattttct
    10861 ttatttgcat tcagtgccct ttttaatgca ttaaattgta gagagtttgg tttaaatagt
    10921 acaataccaa atttctttaa aaataaactg gcattgcaaa taatagtagt tacagggata
    10981 attcaaataa tatttacaca agtgttccag agtttcttta attctgtttc tttggacttt
    11041 gatatgtgga taaagataat actatttgca tccactatcc tattgtcaaa tgaatttgta
    11101 aagctaattc ttagaacaat gaaaaatagt agaactatga actttaatag taaaaattaa
    11161 ttcctatgaa accgcaaagt actatatatt gtaactttgc ggtttttgtt tgtttaatgt
    11221 tgttttatat taccattttc atatttaatt ttttattaat ctaaacacaa tcttccattg
    11281 taattgcttc atctgtgtca acacattcaa agtcaatatc taatttttgt aatattttgt
    11341 atgcctcttt ttgcaaagga gtatcaaatt caataggtga ttttgtgtaa atataactga
    11401 taaaaaacat gatttatttc attcctttcc atttaaaaac ttatagttat agtgtatcat
    11461 aaggttataa aatatagata aatatagtat aaatttaaaa tatgtacaga caaaacaaaa
    11521 aaatcatatt ctaagtaata cttgggaaaa atgatattat cataataaat aatctttgta
    11581 aaaacttata tagaatctat taaacagaca ctaagccata aaaactttac taaatataaa
    11641 attcgtatct aaatagatat atagaataat attttactga agtagagtga tttttaagaa
    11701 ttttatatat aaattatgtt atcataaata aagatataga ttataaaatt tgttaataaa
    11761 ataaagggca gggaggtaag tttcatgttt tccaaaatct tagtcaaaac atttatcaga
    11821 gatagcgaaa acgttcaaaa tacagatgtt agaaataagt atggttatgt agctggtgta
    11881 gtaggtatat tatccaattt acttttattt gtgataaagg tattcatagg aatgctcaca
    11941 tctagtatag ctataatggc tgatgcattt aataatcttt ctgatatggc atcttcagca
    12001 ataactatga taggttttaa attagcaagt aagccagctg ataaagaaca cccttttggt
    12061 catggaagaa tagagtattt atcagcatta atagtagcat ttatggtgat gttggttgga
    12121 cttcaatttg taaagtcatc tatagaaagg atagtaaatc caattcctgt aaaatttgaa
    12181 gtaataccac ttatactttt aattgcatct ataatgatta aaatctggtt gagtcgtttt
    12241 aataaattta tgggaaataa aattgattct tcagccttaa aagcagtttc acttgatgct
    12301 ttaggagatg tatttacttc aagttgtgta gttatatcat ttattgtagc tagatttact
    12361 aattttccca tagatggata tgttggtatc gtagtatcat tagtgatact ttatgctggt
    12421 ttctctcttg taaaagatac tataaatcct cttttagggg aagcaccgga tgaagaaatg
    12481 gtaaattcta ttatagagct attgttatct tataaatata taataggtac acatgattta
    12541 attattcata actatggagt tggtagatgt attgcatcta tacatgcaga aattccatcc
    12601 aacatagata taatggaaat acatgaaatt atagatactg cagagagaga aatatctgaa
    12661 aaattagata tatatctagt aattcacatg gacccaatat gtcttgaaga taaagaagtt
    12721 atgtcagcca aaaaagaact tgaagaaata cttaagaaaa atagtcttgt aaaatctatg
    12781 catgatttta gaatagttgg aaaaggaagc aagaagaatt taatatttga tatagtagtt
    12841 aatccttccg aattttccaa aaacatgtca gaagatgact taaaagaaga tataacaaaa
    12901 ttagttaagg agataaatcc tgaatataat tgtgtaattg ttgtagataa ggattttatt
    12961 taattattaa ttaatctcat cataaggtaa aaaatgtaaa aaggatagac aatagtatag
    13021 aggcataata taatagaagt atataattgt atttttaata taaataaagc aattttaatt
    13081 gtttttatga aatattatag agatgttgaa gtatattcta ttattgggca ccttatggat
    13141 atactgagtc agtggtgcaa ccggctatga atataaattt atttattttc atagcttttt
    13201 ttgtataatt ttttagtaaa aattacttta tatacaatat taatggaaga ttagattata
    13261 aattataggt aacctaaagg ggtgagattt acatgaagaa aggaaataga aaggcattat
    13321 taattagttt aattatgatt ttaagtatgg tggtgtctac aatatatcca actgtatctt
    13381 atgcttcaga attaggagag aatagtcaga ttcaaagtgg ttcaactaat tcatcaactg
    13441 gagaggagaa ggaaagcgac aataaaaagc cagaacagac tccagagaaa gataaggcaa
    13501 cagataataa aaaaccagaa cagaccccag aggaagaaaa accaacagat aataaaaaac
    13561 cagaacagac cccagaagaa gataagtcaa cagataataa aaagccagag caaactccag
    13621 aaggagaaaa gccaatagat aataaaaagc cagagcagat acaagaagaa gataagtcaa
    13681 cagataataa aaagtcagaa caagctttag aagatgaaaa accactagac aataaaaata
    13741 cagaaaaaac tccagaggaa gataatctat tagaagatga aaatttattg aaagttttag
    13801 aagaagaact taatgaagaa aatgaagact atggatttgt tgtaaaaatt aataacaata
    13861 caatagaaac ggagtcaatg aaaaaaatat catttaattt aacttatact ccaacatcaa
    13921 aaggaataca agcaggagat tcaataactt ttaaggtgcc agatgtattc aataaagtta
    13981 atttagatta tacaagtgaa tgctttgata aaacagaaag taatggagag tatacactta
    14041 catttagaga attacctaat ggccaaagtg taatgcaagg taaaataggt ttggaagcat
    14101 atgttaaaaa ggttgatgaa gatactaatg caaaaataca tattgaaact actggaaaaa
    14161 tagaaagtgg aagtggagat atagatgtag aaataaaacc tggagataaa acggatgttc
    14221 cagatgctaa aggaacactt aaaaagcttg ttgaaggaaa aaaatcaact actgtattta
    14281 tgccagttaa aaataaagat ataaattaca gtatacaagt aaatgaaaag caggaagagt
    14341 taaaagacat tattttgtat gatgagcttc ctgaaggatt gacattgata aatggttctg
    14401 taagtgtagt aacaagtgat ggaaaagaag ttagtgattt taatattgaa cagagtaaaa
    14461 attctatttc agttaatttt ggaaatatag ataaatcgta tactgttaag tataaggcta
    14521 gaatcagtga caaaaatgca aaacatggta ataaatataa gaatgtagct aggattgaat
    14581 ctgatggtaa gaagatacaa gaagatgatg ctactgtaag tatatttgat agaggagatg
    14641 attatcttct tactaaagga cattcaggag caacaaatat tactcaagtt ggtcaagtaa
    14701 taaactatca gatttcaata aatgatgata aatcaccaat aagtaatgtt gttataacag
    14761 ataatatacc agaaggcatg agattaacta cttcaggaga agctgggcat gattttagag
    14821 ttgtagaaat tccaatgaat gggagctgga ctccttggag taaagaaaaa attgccaata
    14881 atatttcata taaagtagaa gaaaaaagaa atgaaagtgg gcaagtagat aaagttataa
    14941 ctggatttac aattaatctc agtaaagaag aagtggaatc aaagtttttt atagcttata
    15001 cattaaaggt aattagtatt gaagatagct atataaatag agctgttctt gatgcaaata
    15061 atagtgaaat tgataaaaat gatgagataa actttaagaa aaattctgga ttaataagcg
    15121 caaaaaaaga agttgataaa aaagttttaa acagtagtga taaccagatt gttaaataca
    15181 aaatcaatat gtcaacttat ggagtttatg atgcaggaca agtaaatctt ttagatgaag
    15241 taaactcagt tctagaaata tctaatataa aatattctga taatcttgaa cttaaaaaag
    15301 aagctggaga tggaaaaaat acaataagat tagtaaataa atacgaattt aaacagataa
    15361 aagaagggga acctgtgcaa agttgggtta catttgatgc aaacttcaca aatgtaaaag
    15421 ttggggagac tatacgaaat gtggctcaga ttaatggttc aagtcctcca ggagtagaga
    15481 ctactaagca aggttatgct tttgaagcta aaaaagtgga tgctttagat aaaaatgtac
    15541 tttcaggtgc taagttcaat ttagaagatg catttggaaa tatagtagtt aaggatttag
    15601 ttagtgatga agatggtata attcaatcta gtgtaaaaaa cccaggaaca tattatttag
    15661 tggagataat ggctccacgt ggatatgaaa aactgaagga taaagttaag gttgaaattg
    15721 gtaatgaaga tattggtaag atagtagata ttgggaatat agaaaatcta aaacaagaaa
    15781 atccaccagt aaatccacca atacctccag atacagatga accaatagta aatccaccag
    15841 taccaccaag cacagataaa ccaaggaaac catcaagttc atctgataca gagaatacta
    15901 tagttataaa tccaccagta ccaccatcag aagatataat aaatccgcca ataccagagg
    15961 tattgaatcc gccagtacca ccatcagaag agatgataga aactcctgtt aaacagatta
    16021 ttccaatacc agaagtagta aagccatcag tgtcagaaga aaaaaataat aaagttaaag
    16081 atgatacttt ggtaaatcca ccagtaccac caaaaacagg ggatagtact acaattattg
    16141 gagaaatact tttggttata ggagctattg taggacttat cgtattaaga agaaataaaa
    16201 atacaaatta gaccctaaca ttataaaaat ataagttata ttaaaaaata tcttctaaaa
    16261 caatatttaa taatgaaaat attgttagaa gatatttttt tttgaagatt tatgtaaaaa
    16321 aataataact ttttagaaaa aagttccaaa gggatataga caattaaaaa gatataagct
    16381 ataatataaa tataaaatta aatgaggcaa aactagggaa acctagtcac gcaaagctat
    16441 aggggctaaa gtttagtaaa ccatgccagc cagttgccga aaagagtaaa aatttctttt
    16501 tgtatataca aagatactct tgggtatctt ttttattttg aaatttatta ctcccggcct
    16561 aactcaaagg agggaataat atgagaccaa gtaaaaaatt attaatagct ataatatcaa
    16621 tattcttaat aagttcagtt ccagtaagtg cacatgcaga tagtactact atacaacaaa
    16681 ataaagacac acttagtcaa atagtagttt ttccaactgg aaattacgat aaaaacgaag
    16741 ctaatgctat ggtaaataga ttagctaata tagatggaaa gtatttaaat gcattaaaac
    16801 aaaataactt aaaaataaaa ttattaagtg gaaagttaac agatgaaaaa gaatatgctt
    16861 atttaaaagg tgtagttcct aagggatggg aaggtactgg aaaaacttgg gatgatgttc
    16921 caggtttagg tggaagtaca gtagctctta gaataggatt tagtaacaaa ggtaaagggc
    16981 atgattcaat aaatttagaa ttacatgaaa cagcacatgc aatagaccat atagtattaa
    17041 atgatatatc taaatcagca caattcaaac aaatatttgc taaagaaggt cgttctttag
    17101 gtaatgtaaa ctacttaggt gtatatccag aagaattttt tgcagaatca tttgcatatt
    17161 actatttaaa tcaagataca aatagtaagt taaaatcagc ttgtccacaa acttatagct
    17221 ttttgcaaaa tttagctaaa tagagtttta ataaatttaa aataaatata aataaagaga
    17281 tgctattata atagcatctc ttttatattg cgtttaaatc taataaattt tgtcttatgt
    17341 ataactatga tagattatca attactactc cttatacaag gcttcaacgt gtttattatg
    17401 catttgagta attatcgtag gtataataga aattattgaa ccaattacta tcaataaaat
    17461 tcctataacc atgtttatag ggataatttc tttaagtatt aataatgcta gtattggaga
    17521 tattactggt ttaaagaaga atactaaaga tgtaagacta gtagatgtag cttccattgc
    17581 cataaagtaa agcgcataac caacacctgt aacaaatacg aatacataaa tcataatggg
    17641 catattgtgt aaggtatatc cactaaaaat agggatattt gcaaatacat gaagcttatt
    17701 tttatttaat atatctgcga taaaattaat tttagtgagt aaaaccaata tcaacatttc
    17761 caaactacca aatataaagc taaagcaggt tacaactatt ccaccaaatt ttaaggtttt
    17821 tcttttacca aatacagtat acattgaaaa aataatagca gaagataatg ttaagaatat
    17881 tccactaaga gttagttttg tatgcaatgg atttattata actataattc caagaacttc
    17941 taatataagt gaaattacat tgtgcctata tattttttcc ttcaaaatta agtaagctaa
    18001 aagcattaca aagactggat tacaactgaa caaaacagca actacagatg cctttgtgtt
    18061 tagaacagca agttgataca atgacatact tactactaca cacataaaac ctaaaaatgc
    18121 aaagtatttt aagtctgatt ttgtaataga aagactaaga gctcttaatc tttttacagc
    18181 aaaaggtagt aagactaaac cgccaacaaa aaatcttgat aaagttattt gtaagggatt
    18241 aaagtcatta gtgacagttt ttagactaat ttccattgaa ctaaaaaata aggtagtcag
    18301 tattatataa acataacctt ttttcaagtc aaacacctcc ttgtaattta tattagtact
    18361 atagataaga atattcaagc atattggaat aatatggtat gtataaaata tagtacataa
    18421 aattataaac taccttaata tagaatgtaa gtattctata ctaaggtagt taattatttg
    18481 aattactttg atttagttat tttaaattat ataatttggc taatttttca aatgctggta
    18541 aagaactttt aactttatca taagatacac aatatgatac tctaaaatga cctggacaac
    18601 caaaagaaga acctggtact aataataaat taaattgttt tgcatcattg cagaattttt
    18661 tatcatcttc aattggagct tttgggaaca aatagaatgt accattaggt ttaacacaag
    18721 aatatcccat atcgattaaa ctattatata gaaggtcaaa attcttttta taaatattta
    18781 catcaacttc tgcatctaaa cttcttgcaa taaccctttg aaaaagagaa ggagcattta
    18841 caaatccaag tattctattt gcaacattta atgaagacat aacatcatca aaatcatcca
    18901 tttgcccatt agcaacaata tatcctatac gttcaccagg tagagataat gatttactat
    18961 aagagtatcc tataaatgaa tttctatagt attttagtac acaaggaact ttagcaccat
    19021 cataaattat ttctctatat ggctcatcag aaattaggta tatgcttgta ttaaattctt
    19081 tttgttttga atccaataat tctccaagat tttttaataa ttcttcagaa tatatgacac
    19141 cagtaggatt atttggagtg tttattatta aagctcttgt ttttggagta atagcatttt
    19201 ttagagcatc taaatctggc tcaaaagtct ctatatttgt aggaacttct attaattttc
    19261 catcatagtt ttctgtataa tttttatact caccaaaata cggtgcaaaa gcaataactt
    19321 catcaccagg atttaaaaga gtttttaaaa taatatttag accaccagca gcaccacaag
    19381 tcataattaa attttctctt gtaagattta atccatcttt tttatttatg tgttcagcaa
    19441 tagcatctct tacatcctca tatcctgagt tgttcatata tccatgaact aaatttggag
    19501 attcttcatt taaaatatca tttataactg ctttaatagt ctctggaggt tcaacacttg
    19561 gatttcctat actaaaatca aaaacatttt cctcaccata aatatcagaa agtttctttc
    19621 cttcttcaaa catagctctt ataacagaac tatttgcaac taaagtttgc attttattag
    19681 atatcatatg taacactcct ttttatagat ttatattgct tatgtatata ttaccatatt
    19741 tttactgaat ttagacaatt ttcgaaaaat tacttgtttg aaaatataaa cttttatata
    19801 aaatatattt atgtacagtg catattaaaa attccaattt ctaattttat catgattatt
    19861 ttatataata gttttttaaa gaattaataa aacatagatt tagagaataa aatattcttg
    19921 actatattaa ttgtttaata taatatagtt gatatagtca aacatattat aattatagaa
    19981 gggaaattta agatggattt taaaaattta cagtatgaaa gtttgtcata tattatttgt
    20041 aacttgcaaa aaaactttaa actttattgt gaaaaatgtt taaaaccata taaacttacg
    20101 aatggtctat atttctattt aatttatatt aataaaaata ggaattgttc tttgaatgat
    20161 gtttctacag aatttgaagt tgataaagct catacaacaa gaactatatc taggttagaa
    20221 caagatggat acatagaaaa aatccaaaac cctaatgata gtagagcttt tcagttgaga
    20281 gtaacagata agggtgagga agtattaggg gatataaaaa acatattttc aaagtgggat
    20341 aatcacataa aaaaagagtt ctcggataca gaatataaag aactggtaaa gaatttacat
    20401 gtggtaaagg atataaaaac agctgtggag gaagaatagg atgtattgta atttattaaa
    20461 accaattaaa attggaaatt tggaattaaa aaatagagta tcttttgcac ctacaagcat
    20521 gggtcttaaa ttggaagaaa aaattaaaaa gttttctgat attgctaagt caggagtagc
    20581 tctgattact ctaggagatg tatctattag acctagtttt cataaggtag ctataagcct
    20641 atcggatgaa gatggagtca ttaaatacaa gaaaattgta gatgaaatac acaattctgg
    20701 tgcaaaggta tcagcacaac tattttgttc agattatgat gttaatttaa ttaaagatac
    20761 tatgaatatg ggaattactt cacatgatga gataaaaaaa ataatgaatg acggagtaaa
    20821 agattacata acaaatatgc ccaaagaaga aattaaaaat attataaatt tatttaaagt
    20881 aacagcattg aatgcaaaaa aagcaggttt tgacatgatt caaattcatg gtgataggct
    20941 tgtaggaagt ttttcatcaa gtatatttaa taatagaaat gatgaatatg gtggaacgtg
    21001 tgataataga agtagatttg caagcgaaat aattagtagt atccgagatg aagttaagga
    21061 cattccaata gactataaat ttgcaataag acaagaaaat cctcattatg gaaatgcagg
    21121 agttttactt tcggaagttg agtattttgt taaaaaattt gaatccctag gtgtaaacag
    21181 ttttcatgta acacttgcaa atcattcaaa attagaagat actataccaa ccaataatca
    21241 tccttacttt aaagatgaag gctgtttttt atatttagct gatgaggtaa agaaacacac
    21301 aaatcttcca gtatgtgggg ttggaaaatt gtctagccca gattttatag aaagtattat
    21361 ttcaaataat agagtagata tggtttctat gtcaagacaa ttactagcag atagtaattg
    21421 gttacagaaa gtaaaagatg gaagagtaga tgagattaaa aagtgctgtt attgtaataa
    21481 gaagtgtgca gatgctttac aaacacgctc tcagtttgga tgtatactag attagaatat
    21541 ccttataaaa ataaataatt tgttaaggtt aatatatttt tggtgattta gataaatatt
    21601 tagataaaga ggaggagaca tcatgaaaag tttagtttta tattcaagtt taactggaaa
    21661 tactaaaaag attgcttata ctatatatga tgaaattcaa gaagaaaaag acattaaaga
    21721 tgtaaatgaa ttagttgatt atggtattga ctatgaaaat tatgatatag tattcttagg
    21781 ctattgggtg gacaagggaa tttgtgataa aaattcaaaa caagtgcttg aaaatattca
    21841 caataaaaag atagcactat ttggaacaat gggagcaagt aaaaaaggaa gttatggagc
    21901 tagtataata gaaaagatag aatcaattat cccaaaagat aatgaaatac taggttcatt
    21961 catatgtcaa ggtaagatag cagaaggatt gaaagcaaaa tataaagaga tgttaaaact
    22021 ttctcctgac aacgaacata ttcgccaaca attaaataac catgaggaat ctcaaagtca
    22081 tccagatgaa caagaaatat atgaagccag tatgtttgca aaaaatatga tgattaaagc
    22141 tagtattgtg tagtagttaa acaataagtt catataagat atagaataaa taatttagta
    22201 aaaaagtata gcaaataatt tgctatgctt tttttatgaa atcaaaaaaa taataaaaat
    22261 acttgactgt aaaccttgtt tatacattat tttatagaaa ataagatagt tttaaatata
    22321 aaaaaagata tattattgaa aggagatatt attgtatgaa aacttataaa agataattag
    22381 tttgatttta agatatacta aatatacaat tttatagagt gaaaatattt aagactatat
    22441 tatagttata aattaataga atagagaggt agtgtttaaa ttgcaataac agtatttcca
    22501 aagttgccac ctcaaattcc tatccaatgg aataatggtg tagctaatta tgtagataaa
    22561 aaatttattt atgcctaccc tgtcatttgt gttgtattca gaatcctttt acgtccattt
    22621 atatggagat ggttacaaat acatgctttt atagtgatac tattgcagat tatttaacga
    22681 attttatgtg ttttgtagcg ttgtctatag aaatattctc tatatttttt atttacaata
    22741 ttgccagaca tgttacaata attattttta tagatatact tgtatttatt gctttactga
    22801 taataggtgt gtataaatta tttaaaaaat acaattaatt tttaaaaata agtaataagt
    22861 gatagtgtaa aaagtagcca ctgcaaatta tggattgtag tggctatttt attatttgta
    22921 tatgaaatta ttttgttttt ctaataagtt acgatatatt atggtttatt tcactgataa
    22981 aaggtttagg tattttaaag taaaaagttg tatattctaa taaaatttaa acattttgat
    23041 tctttaaaat tagagaaaat acattgcttt tattaaaaaa ttaaatatta agaatttaat
    23101 ttctgcatct tatttagaaa agataagaaa tcaactttat atattttttt tggccttcct
    23161 ctttgattat cgagttttgt atttacaata gttgcaattc ctgctttata taagatactc
    23221 aatattctat tagcacttct aagagttatg ttcatatagt ctgctaatat ttctgctgac
    23281 acattatttg aatttctatc attaatcata cacatgattt tagatacatt tgtattagat
    23341 agaggtataa aagaagcaac tttattggct attgagctat cttcaatatt attagaattt
    23401 ttatttgaat ataaaggacc tattgtatca ttagtatcat ttactataaa agtacagttt
    23461 ccattcaaag aagcagattt accattagct tgacgagcat tttgctctgc atgaatattt
    23521 gtatttccaa tgccccaacc tatatttaag ctatatttaa tttcttttaa atcttctgaa
    23581 ataaaaaaat ctgtatagtt attggtcata ttgtttaaca tttctttagt agtgtgtatc
    23641 tctattttca tatcattttt tttgacctga actccataga atccatgtct tgatagagaa
    23701 tttaatatag tgttatatat ttgattttgt atctcatcat ttttaaatat caaatcttca
    23761 tttatgtact ctgaactatt tactgtaact atacaagttg cagatttatt tttgtctaag
    23821 ttttgtaatg ttatttcatt caccaaagat ttaaatgtat tcaggattga ttctgtagat
    23881 ggaaccatgt aaatacaatc atatccaagc ttattaagtt cacaactaat gatgtaattt
    23941 ctagtaaaag aaaggttaat cttattatct cgcaataagt ctaaatgtat ttccaataat
    24001 ttatcattta attttttaaa gtcatataca ggttgaggga tattaaaatc tttaattgta
    24061 tatggacatt tattagagtc tattaatgaa tatatatcat aataattgtt gtcctctctt
    24121 aaaaaatcca taaatgattt tgtaaagtca atatcttttt ggctatttaa taatttgaat
    24181 aagtatttat aaaaatctct ttcatcaagc tttaaaaaat ttaatggaac ttttatgtct
    24241 tttatattta ttataagttc actatatgga actataccac tgcatacaaa tccatcaaaa
    24301 ttatctactt gctcatagta tatattaggt aaattattaa aaggattctc ccctttaagc
    24361 tcaataaatg taatttcaca agtatcattt aattctttaa gagcttgttt aaacttatct
    24421 gacataaaat aagatgttgt caaaaaacca attttatatt tcataaaacc tcctttaaaa
    24481 tttcgagagt attgtaacta atcaatcaat ttataacctc attttataat agtagataaa
    24541 acgcgtcaat ttattttaca aaaggaaaat tgaaagaaaa atcagaaaat ataaaaaaag
    24601 actgaaaaat atgttggctg tcaagaataa ttgtgctatt ttgtaattaa ggactagtct
    24661 tttaagattt cctaaaataa acttagattt taggaaagaa atacatttta tttgaaaata
    24721 aaggagaaga ttatgaatac atatttaaat gaaatttcta agtttgttga tgagaataga
    24781 gaagaaatag tttcattatg gaaagagatt gttaatatgg aaagctatac acattgtaaa
    24841 gaaagcgtaa ataaattggc taagagatta aagtttgagt ttgaaaaaga aggtttagat
    24901 tgcgagttag tagatgttgg agataatggt agtacattga taggtacact tggttccaat
    24961 atagataaaa agccaataat tttttcaggt cacatggata ctgtctttga aacaggtaca
    25021 tttggagaga atccatttaa aataatagaa ggaaaagcat atggcccagg tgttttagat
    25081 atgaagggtg gtataataat ttccttatat gttattaaag ctttaaataa aataggatac
    25141 aaagaaagac caataaaaat agtattttct ggagatgaag agataggaca taaggattca
    25201 acaggtgcag atgttatatt gagggaagca aaaggtggtc tttgtgcttt taatatggaa
    25261 actggattag tagataatag tttgtgtgta ggtagaaaag gaagaatagg atgtaatata
    25321 catgtaaaag gtgtagaaac acatgcaggt aatgattttg aaggtggaag aaatgctata
    25381 gaagaaatgg ctaataagat actacgcata caaaaattga ctaatttaga tgttggaact
    25441 acagtaagtg taagtgtgat taagggtgga agagtggaaa attctattcc agaagattgt
    25501 agtataaaag tagaccttag atttgaaaaa gtagaagaaa tggagaatgt taaagaacaa
    25561 gttagagaga tatgtaaaga aacttacata gaaggaactt ctacagaagt taattttata
    25621 agtgaaatga tgccatttga aactacagaa gatgttatga gatttcatac atttgtaaat
    25681 gaagtgtcaa aggaaaatgg atttggagaa ttaaatgcta agagacttgg aggtagttca
    25741 gatgcaagtt atttaactat tgcaaacgtt cctacaatct gttcatttgg tgtaagaggg
    25801 gaatggaacc acacatcaag agaatatgca gtagtaaatt ctatgtttga gagggtgaaa
    25861 ttaatttcta cagttatttt aaacttagat aaatttgagg tataaagggg gattttatat
    25921 ggatgtaaca gctggagata ataaggtaac atggaaaggg tatttatctt tatttgtatt
    25981 gatagtatta ttttcaggag tgtttaaaga tagtcaaggc ttccttaaag cattagattt
    26041 tgctaatctg acaggttcat ttggtactat tgcagatggt gtagacttta tgggtaaagg
    26101 aggaacggga gcaaaagatg gtttcttatt tgctctgact ttgattccaa ctgtctgttt
    26161 tgctgtaggt cttatagatg tagttgaatc tatgggagga atgaaagcgg catcaaagat
    26221 atttaatcca ttgcttagac ctctgcttgg tatacctgga atagcaggaa tagcatttgt
    26281 atcttcattt actagttcag atgtagcctc aataatgacg aaagaattat ttgaaagtgg
    26341 ggaaataaca gatgatgaaa gaactatatt tgtagcatat caatatgctg gttcagctgt
    26401 tatactgaac acaataaata ctcaagctcc acttttacca atagcattat tggcattagg
    26461 tccaataatc cttatagaga tattctgtaa aatacttggt gctaatctag ttagattaat
    26521 cataggtgta agaaataaaa agaataaggc taaggaggct gtgtagtatg agtcaacaaa
    26581 atactggaaa tgcaattgta aatagtaaac ctaagaaaac tgctgttgag atatttatgg
    26641 ggggagcaaa aaaaggtttt tacataggtg ttgaacagat attaccagcc atgataatgg
    26701 gttatgtaat agtgcagttt ttacaattaa ctggtttagt tgatatttta ggaaatattt
    26761 ttggaccagt aatgggtatt tttggactac ctggggaatc tgttgtagtt ttgatagcag
    26821 cattctttgc taaggcagca ggagcagcta cagctgcaaa cttatttgca caaggtctaa
    26881 tcactgcacc acaagcaacc atattaatta tgccatgtat gttaatgggt actctagttg
    26941 gtcattatgc cagaatagtg cttgtagcaa atgccagtac taagtataga atgttactgt
    27001 tggcagttcc tatatttgat gcaatagtgg ggatgttaat agtaagagtt ttactcactg
    27061 ttatgggatt aatgtaaata acataaaatt ataacctaac tataaatgat atataagaat
    27121 taatatatac aaggattaat gagtagtaat tgatatatag taataaacta agatagtaat
    27181 taatgtgtat attaattaaa aaaagagagt atctcaaagt gaacttttta gttcataaga
    27241 tactcttttt gtatgaaatc aagtatattt tcataatttc aacaatgaaa gagaggctta
    27301 tctctatttt gagacagcct cttcttttaa atttatattt ctagaattta tgttttttat
    27361 ttatccaaca ttaacattag ttattagaaa taattttaag aaaaatatac tttcatgtta
    27421 atatcagaat aaatgtgttt ttaaaggatg atttgttttg aattttcacg aatatctact
    27481 tctatgactt tttctattgt atctcctcta aaacctaata aattacttgt caagtttgca
    27541 gtggaacaag aatgattagg tataattctt attctatcac caatttttat atcagtttca
    27601 ccatttactt taatttttcc aacttcttct gaaagtgaat caatagtaag ttctggatgt
    27661 ccacataccc taccataacc aacaatacta ctatttccat gtgcaccttt atcaagtcca
    27721 aggcacttac ttccacaatc aattaaatag tatccactgc tattttttga gattacagtg
    27781 cacacaacag taagagcaca catatcttca gtagcacaac ctaaacctat ctgtatagca
    27841 tcatagtata catagtttcc aggtcttaca gcagttatag ttttagaagc agcttcagat
    27901 tcaactgttg gagtactacc agaagcgaca atttctatat taaatccatt atcagttaga
    27961 gctttgtatg ctttctccat agaagaatct tcttgaacac aaacatctgg aacaccttct
    28021 ggagaactac aaccatacac ttgacctgga tgcgaactta taccaacaat cttgatgttt
    28081 tctagcttag aaatttcagt agcaagttca accacttttt ctggttcaac cccaaaacgg
    28141 tgtaagccac aatctacaat aataagtagc ttgcactcta tttttgattt agaaaaagct
    28201 tcactataag ccttagcaca ttctatatta tctgtagcac agattaaatt tatttttgat
    28261 gccatagtca caattctatt tatatttact tctcccatgt aaggataagc caacataata
    28321 tcttcaaaac ctgcttttat cagagcttca ccttcatcaa gtgttccaac tagaaaacca
    28381 ctagcacctg catttttttg aagtctcgct atgtaagtac ttttatgtgt cttaagcata
    28441 ggccaaagtt gtttattatt atttgtacaa atttgttgaa ttttatctat attcttttct
    28501 aactggtcta aatctagcaa aaaatttgga gtagcgactt ctgaaatttt catgttattt
    28561 accacctttc aacaaaagta aattaactat aagtattata attattatat tagtaatatc
    28621 ggctttataa tattaatagc ttataattat attatagaac tagcgttaac agagagcaat
    28681 atttatttgt aataattaga attttcaata agttagtgta gttttaaatt atttatataa
    28741 tatattttta attttatgca aagtttattt aaattagttt gaggatttat taatagggta
    28801 ttattgaatg agtaaaaaag tgatgtttac atagaataat cacactataa attaatttag
    28861 aacctaaaaa attaattgag caaattggaa aagagaagga taaaaaagat atttggatag
    28921 ttggtggagg cgatatagta aaattattta tgaaagataa cttaattgat gattattata
    28981 tatatatatt gccaataata ttggggagag ggatttcact atttaagtct ggtatagaca
    29041 agactaattt gaattttaag aaagctagta atattggaga acttgtaaaa ttggaataca
    29101 gtaaaaaaca aaaataaatt acatgccaat ttgttacaat gttatgaaac gaagcattta
    29161 aaagactttt ggctatatga attgacatta cttgtcaaat atgttaacat ataagcgact
    29221 tgtcggaggg tgtattaaaa agaatatgct ggagaagaga gtgcgttaac tcttttctcc
    29281 tttttttata aaattaagca tttataaatt tagtattatt acatgattac actatctcaa
    29341 aaattaagaa aggagaaaaa tatgaaatta attttaagtt atttaaagaa ttataagtta
    29401 ttaatagtat taaacatact ggctatattt agttttgcat tagtcgagtt ggggattcca
    29461 actataatag ctaaaataat tgacaatggt attgcaaatc agaatatagc ttacataaaa
    29521 caaatgggaa tagtgattgt tattatttct attatagggg ttgtaggtag tatattgttg
    29581 ggttattgtt ctgctaaggt atcaacaagt gtaaccagag atataagaaa tgacatattt
    29641 gagaaatcac aagaattttc acatactgaa tacaataaat ttggaatttc ttcaatgatt
    29701 acaagaacaa cgaatgatgc atttcaaatt caacaatttg ttaatatact actaaggact
    29761 gccttattaa ctccagttat gttcattata agtataataa tgacaattag aaccagtgtg
    29821 gaactttcta tagtattggc tatatcagta ccttttataa ttattggagt agcaataata
    29881 gcaaaagtat ctcaggcaat atcttcaaaa caacaaaaag gactagataa attaaacctt
    29941 atatcgagag aaaatttaac tggaataaga gtaattagag cttttggaaa tgatgattat
    30001 gaaacagagc gatttgaaaa aacaaataca tattatgcta atgtttctaa gaaactattt
    30061 aagcttatgt caattactca accagcattc tttttactgt taaatatagc tgttttggct
    30121 gtattttgga tttcaagtga gaagataaat attggagagc ttcaagttgg acagttggtt
    30181 gcatttttgg aatatctttt ccatgccatg ttttcaatta tgcttttttc aatggtattt
    30241 attatgtatc caagagcaga ggtatctgca aatagaataa aagaactatt aaatgaagaa
    30301 ccattgataa aaaatcctga aaatggaatt aaggatactg aaaataaggg tatcatagaa
    30361 tttgataatg taacttttac ctatccagat ggagaagctt ctgtacttaa ggatatttca
    30421 tttactgcaa aaacaggtga aacagtcgct tttataggaa gtactggtag tgggaaaagt
    30481 actctaatta acttgatacc tagattttat gatgtaacag aaggtagcat taagataaat
    30541 ggagtagata taagagaata tgatttaaaa gctttaagaa aaaagatagg attcatacct
    30601 caaaaatcac tgctttttac tggtagcata gcaaataata ttagatttgg gaaacataag
    30661 gcaggagagt cagaattaga atattccgca aaagttgcac aagcatatga gtttatatca
    30721 aaaaaaccta gaaagtttga tgaattgatt agtgaaggtg gagcaaatgt atcaggaggt
    30781 caaaaacaaa gactttctat tgcaagggct attataagaa gacctgaaat atacattttt
    30841 gatgacagtt tttctgcact tgactttaaa actgatgcaa tacttcgtgc taaattgaag
    30901 aaggaaacaa aagatgcaat tgtattgatt gttgcccaga gaattagttc tattattgat
    30961 gctgataaga ttatagtact caatgaagga caagttgtag gaatgggaac tcataaagaa
    31021 ttattaaaaa actgtgaaat atactatgaa attgcaacat ctcagttaaa aaaggaggaa
    31081 ttagaataat gaaaaagtct aaaggtttta agaaaacaat tggtagactt cttccatttg
    31141 taaagaaata taaattttcc tttattatag ctattatatg tataatttca gcagctacta
    31201 tgaatgcact tgcacctaaa actgaaggat tgataataac acaattgact aaagatgtaa
    31261 ttagtatcgc aaaaggagta ccaggggctt ccgtaaattt tgattatgta acaaaaatat
    31321 tggtgatttt agcatgtata tattttgcca atgcaatttt tacgtatact tcaagttttc
    31381 tacttacgaa tgctattcaa aacacaatga gagatttaag aaatgaagtc gaaaataaaa
    31441 taagaagatt gcctattagt tattttgata gtaatagttt tggagatgta ttaagtcgta
    31501 tatcaaatga tgtagataca atatcaaatg cattgcaaca gagttttatg caaattgtaa
    31561 actcaatctt agtcataatt ttggctcttt caatgatgtt tacaattaat atatatatgg
    31621 ctcttatagc tctttttata attccaatta gttattttgt atctaaattt gtagtaaaaa
    31681 aatctcaaag tagattttca ttgcaacaaa atgctcttgg aaaattaaat ggaaaagtac
    31741 aagaaatgta tactggattt aatgaaataa aactatatgg taaagaagaa gattctataa
    31801 aagaatttaa aaaagtaaat caagaacttt gtgaaaatgg atttaaggca caatttatat
    31861 caagtatgat gaatcctatg gtttcattag taacttactt tggaattgca gctgtggcag
    31921 ttgtcggttc tatatatgca gtaagtggag gaataacagt aggaaattta caagcatttg
    31981 tgcgttatat atggcagata aatcagccac tttcacaaat gactcagtta tctacagtaa
    32041 tacaatcttc ttttgcagct atagaacgtg tatttgaaat tttagacgaa gaagaggaaa
    32101 tacctgatgt tgaaaatcca gttaagatag aaaatgtaaa aggaaatgtt acatttgagc
    32161 atgttaattt tggatatgga gaaaattcaa ctttaataga agatttaaat gcagaagtaa
    32221 aaagtggtca gatggtagct attgttggac ctactggagc aggtaaaaca acattaataa
    32281 acttattgat gaggttttat gatgtaaaga aaggtgctat taaaattgat ggagtagata
    32341 taagagatat gaaacgtaaa gatttgcgtt caatgtttgg tatggtgctt caagatacat
    32401 ggttatttaa tggaaccatt tttgaaaata tagaatatgg tagatttggg gctactaaag
    32461 aagaaattat acaagcagca aaagtagcaa atgttcatca ctttataacc actcttccag
    32521 atggatacaa tatgttttta aatgaagaag catctaatat atctctaggg gaaaaacagc
    32581 ttttaaccat agctagagct tttatatcag acccttcaat attgatactt gatgaagcta
    32641 cgagttctgt tgatactagg ctggaactta tgcttcaaaa agcaatgaga aatcttatga
    32701 atggtagaac tagttttgtt atagcacata gactttctac aattcgcaat gctgacttga
    32761 ttttagttat gaataatgga agtattatag agcaggggaa tcatgatgaa ttaatggaaa
    32821 agggtggatt ttatgagaag ctttataata gccaatttgc agacaaagaa tcagaataga
    32881 taacataaac ttttgtataa aattttgtat caaattcaaa tattaatcat ataattaaaa
    32941 agagtatcag taagttgttt ttttatagat ttactgatac tctttgattt taagactaaa
    33001 ggtatttatg attggtactt ataattcaca actagtcttg ttacatctga taaccagaat
    33061 tacagtactt ttctttttta cctttttgtt ctctaacata taacaattcg cttaacttat
    33121 caatgtaata accatcatca cttatgcgaa ttaaactagt cagataatat ccatctttgc
    33181 ttctaggaat ctttttattt attgctaaat caatagctgt ctttgctgct tcaaaatgct
    33241 ttaaatgtgt atgaccttct tcaaaactct ttctagtatt ataaactaca taaccttttt
    33301 taacttgaaa aactataaat tctttctttt catatatttt attaaacatt caatcagccc
    33361 ttctcaagat agttttatag cataacatta gaagcttcta aaaaatttta caaagaacat
    33421 aaatatttta taaattataa atctaaaact ataatttata aagataactt tctgattatt
    33481 tatatgataa ttcatataga ttaaaagtaa attatcttta ggttaaacaa aagtaaaata
    33541 aaagactagt tataatattt atatgaaaaa gtacaaaata tagtatatga atatagaaaa
    33601 tttaaattat ttatttacag tttttatttc cataagtata tttaccttca gataccttgt
    33661 catcatggaa atatattctt tcacaacttt tatctcccca tagaatgttt tttgttattt
    33721 tatgggaagt atctcctgta aatacttcag ctacagtagt atctgtaaca tcacagtaaa
    33781 gagaagcaaa ttttttaaac caaggatttt cctcaaatct taaaagccag gaagctccat
    33841 aggggcaatg taactcttca aattcaggaa cccatcctag aaatggaaca tcagttgtgc
    33901 taataatatt gtctgcgatt aattcatctc cattttcatg agctctctct ctcatagcaa
    33961 tagctctttc ttcagacctt tcaaataaaa cttttcttaa tatttcgcta gttttatctt
    34021 caccaaactc ttcaaataac actttggaaa aataaaaata taaatcacca aaatgtttgc
    34081 atgccatacg aacttcttca atagcataat ctttgtcaat agtttcatta gttacaacat
    34141 cttcgttcaa aaaaatcact ccttagaata agtattagtt acaatataat tatagaatat
    34201 tttttataaa ttaggaaatt aatttgagat agatatattt ttaataaatg aaggtataat
    34261 gctaaaataa cttattgtta caaaaatctt ctaaattaat gtatttagga gtttccatat
    34321 gaatgagagt agttctgtaa atatcttcaa tacatccatt tgcaatatta ttaaaaatat
    34381 ctgtgtgctc ttttaatgtt gtttcaagcc ttccttcatg tgacaatgaa tcgagagcta
    34441 atttttttat tctgtacata taattgttaa acattttgtc aaattcagta ttttgagcat
    34501 atgaaactat agttgtgtga aataatgtat catattctgc aaatgagttt aaatttttat
    34561 cacctaataa aatatctttt tgtttatcta aaagatgttt taatttactt atagtttcta
    34621 ctgctctagc ttctttgtat tgacttgtaa ttaaaaaagt acaaaatcct tctattgcag
    34681 agcgtatttg gaatatctct acaatttcat ttgctgtaat ttgatggatt ctaaatcctt
    34741 tgcttgggat tatatctata aatccatctt gactaagtct ttgaagagca tctcttatag
    34801 gtgttctaga aatacctatt tcactagcaa gttttgtttc agaatatatt tcattttcta
    34861 tcatttttcc agatagtatc aattctttta agtagtcata tgcttgttct tgtaatcttt
    34921 taaatgtagc catacttaat aatacccccc taaacaatat aaaaatacaa aaacttatat
    34981 aattttaagc atacaacata gtgaaaataa ggtcaatctt ttgcaattta aattgcagat
    35041 aagattaaaa aatataaaaa attattgaag tgtgaagcat gattaacatt aaaggcagat
    35101 taattaaaac taattgaaat tagttaaaat acaaaactgt aaaagtagta tattatattt
    35161 gaaaacaata gtatgtatta aaacttatat aggatgacta aatataataa atgactttaa
    35221 aaattactat ttattgttaa aataaatact tttatatttt atattgaccg gtatactggt
    35281 atgtagtata ctctaaaaca taaaaaacgt tttcaaaaat gtgaagatat ggagggttat
    35341 aaaatgaaat tgggatttat tggattagga ataatgggaa agccaatggc aaaaaattta
    35401 ttaaaagatg gatgtaatct tttggtgtat gacattaata aatctgctgt ggatgaactt
    35461 atatcatgtg gagcaaaata cgcaagtgtt ttagaaatgg gtcaagaatg tgatattgta
    35521 tttacaatcc ttccaaatgg aactattgtg caggatatat tgtttgggat tgatggattg
    35581 gcaaaaactt taaaagaagg aagtatagta gtcgatatga gttcagttac tcctacagaa
    35641 tcaatattgt gtgcaaataa attaaaagat atgggcttag aatttataga ctcaccagta
    35701 agtggagggg agccaaaagc gattgatggt acacttgctt ttatggctgg tggtaaggaa
    35761 gaaatttata agaaagtaga gccatttttt aatataatgg gaagctcatc tattttaatt
    35821 ggagataatg gtagtggaag tgttacaaaa cttacaaacc aagtaatagt taatttgacg
    35881 atagcagcag tttctgaggc atttgtactt gcagcaaaag caggtgcaga cccagaaaaa
    35941 gtatataagg caataagagg aggcctagct ggtagtacaa tattggatgc gaagatacca
    36001 atgattatga atcgtgattt taaaccaggt ggaaaaatat ctattaattt gaaagatatt
    36061 aagaatgtaa tgcaaacagc acacaattta gatgtaccgc tacctatgac aagtcaatta
    36121 ttagaaatta tgcagacatt aaaagtacat ggacatttgg aagatgacca tagtggaata
    36181 gcacagtatt ttgaaaagtt ggcaggtgtt gaaataaaaa aacacgataa ttaatattta
    36241 aatatatcaa tataattaaa tacatattat taggattaga ttatataaat acagcgaggt
    36301 taagttatca tagtaaaaga aaaataaaat tgataaataa taaagtagaa tgataaaggg
    36361 gtaaagatta tgattacagg atatggactt ttgatagcat ttgttatatc tataggaatt
    36421 ttacttgtat cgattattaa atttaaagta aatccgtttt tagcattact gataacatct
    36481 ataattacag gctttatggt tagaatgcct ataaatgaaa tatcaaccac aatatcgact
    36541 ggatttggaa atacattagg tagtataggt attgtaattg gacttggaat aatatttgga
    36601 aatattcttt cagaatcaag agcaacagaa tctattgcaa aggggttgtt agctaagact
    36661 ggagaaaaaa acagtgcttt agcagtaact acagcaggtt ttttaatttc aattccagtt
    36721 tttatggatg cagcttttgt tataatgatg cctataatta agtatgtttc gagagtaact
    36781 aagaaatctc taatggtttt tgtatgtgca ttaggagttg ggactatagt aggtcatgca
    36841 cttgtaatac ctacaccagg acctttagca gttgcagcaa atgtaaatgc aaatgtaggt
    36901 agttttatac tttattctat aattgttgct tttccagcag ctcttatagg tggatggatt
    36961 tatggaaaac gttttgaaaa atatccagct tatgctattg atgagaatga tagagaaaaa
    37021 aatttagagc agggaaaaga tagcataaaa attgaagatg atgattcttc taaagtacca
    37081 ggttttggaa tttctatgtt ttctttattg ttcccaatac ttcttatact tatatcaaat
    37141 gtatttagta tgttcttaga aaaaggttct acaatgagtg gagtacttgc atttatagga
    37201 gataaaaaca tagcaatact tcttggaata ttggtggcaa taggattttt aaagaaatac
    37261 ataaataaac ctatgggaga tgtagttatt gaagctgctg attcagcagg gctaatatta
    37321 ttgataacag gttcaggtgg tgcgtttggc agtgttataa atgcaagtgg tataggtaac
    37381 ttcttagttg atactatgtc tggtcttagt atatcagttg tagtgcttgg attcttatta
    37441 agtgccttgc ttagaatttc tcaaggttct gctactgtag ccttagtaac aacatcatca
    37501 attttaggac ctacaatttt ggcaactgga atgtcaccag ttttagtagg gcttgctata
    37561 tgtgctggag gagttggttt ctcccttcca aatgattctg gattctgggt actttcaaga
    37621 tttagtggat tatcagttaa agatacttta aactcttgga caattggagg aaccattgct
    37681 ggagttgtag catttataat ggttttattg cttagtgtta taaatggaat tataccactt
    37741 ccaggtctat aaataacaaa ctattgttta tatcatataa attacattta gactatacaa
    37801 attacataaa ggggcgtttt aaatgagaga agtaaaacca attagcatag atatacttaa
    37861 tacttttaaa caagtagatg aggacagatt aaataaactt cttgcagatg agcttaagca
    37921 tttagataga aaaattgttg tattagatga tgacccaaca ggtgtacaaa cagttcatga
    37981 tatttctgtt tatactgatt gggataagga tagtatggaa caaggtttta atgagaaaaa
    38041 tagtatgttt tttatactta ccaattcaag gggatttact gttgctcaaa ctacaaaagc
    38101 acataaagaa atttcaaaga atattgtgga tgtatcaaag aaagttaata aggattttat
    38161 aatcataagt cgtagtgatt ctactatgag aggacattat cctcttgaaa ctaatttatt
    38221 aaaaagcgaa gtagagagat tatcggggaa gttatttcag ggtgaaataa taatgccatt
    38281 ttttaaagaa ggtggtaggt ttactataga taatgtacat tatgttaaag aaggagaaat
    38341 acttgtacca gcaggaatga ctgaatttgc aaaagataaa tcttttggat ataaatcatc
    38401 tgatataggt gagtggtgtg aagaaaagac aaaaggaaag tacaaatcta gtgatatgat
    38461 atatatttcg tcaaaagaac ttagagagct aaatattgaa gctataacag aaaaacttaa
    38521 aaaagctgaa ggattcaata aaattattgt aaatgcaacc gactatgtag atgtaaaggt
    38581 atttacaatt gcgtttatac gagcagtaaa tagtggtaag gaatttatgt ttagaagcgc
    38641 tgctgccata acaaaagtac ttggcggagt gagtgataag gaactgctta ctaaggatga
    38701 attagtatca aaaggtaata ccaatggagg aattatactt gttggttcac atgtgaataa
    38761 gactacccaa cagttagaag aacttaaaaa ttgtaaatat ccaatagaat ttatagaatt
    38821 taatcaacat ttagttcttc aagaaaatgg tctaaaaaat gaggtaaaaa gagttataaa
    38881 agttgtagag gaaaaaataa gcaggggtaa aactgttgct gtttatacta gaagagagcg
    38941 ttttgacttg gatacaaatg ataaagataa acagcttatg atttctgtag aaatatcaga
    39001 tgcagttacc agtataattg gtatgcttaa tgtaaggcca aattttatta tagctaaggg
    39061 tggaattact tctagtgatg ttggtactaa agcactaaga gtaaaaaaag ctacagtaat
    39121 gggacagata aaacctggta taccagtatg gatgacaggc aaagaaagca aatttcctaa
    39181 tatgccatat ataattttcc ctggaaatgt gggagagatt agcacactta gagaatcagt
    39241 tgagatattg atggggtagt atattagtgt attgaagttt aaacgcttaa atttactgtg
    39301 ttaaaagtaa aataaactaa aattataaat taagattaaa aataatagta tattaattag
    39361 ggtttagagt attttacaaa aaatattcta aaccttaatc ttatgtatta gtatttttat
    39421 tatttaactt atgtgtttat tttattattt atctttttta attgatttaa tattttcttt
    39481 gattttattt aaaatatctg tatgcttatt agagcttatg ttggggaatg ctttgattat
    39541 acgtctatga agtaatttgt ggttagacag caaagtatta agtttatttt ttagctcatc
    39601 aatacgattt ttagcatagc gatagttttc tttttcctta agtatttcaa atctatcttc
    39661 caaagtatcc agtctatttt caactaaaga ttcaagtttg ttttctatag tttcttgtgt
    39721 aataagtttg tagtattctt tcttatcttt taattcctta attataagat tgatttgttt
    39781 taacttacca tttaattgta aaattgtatg gactgtgata actaaatcta aaacaaaata
    39841 aatttctata gatatagcaa atataaatag cacatttttg gagatagagt taataaagtc
    39901 aaccacaata ggatgtacta taagcattac aatcaatgat aatacaccaa acataagtga
    39961 gttttccaga caaactcttc catgaatatt aaatttatgt ttagaataat cccaccaagt
    40021 catattaaat attttttcta aaataaagct cgtacaatac tccattatgc tagttataat
    40081 tatactaaac aaaaataaca aaggaatatt gtgtacaaaa ggagtcaagg tagcaactat
    40141 tgctaatgct ccaaatccat atatagggca aaatggacca tttaaaaaac ctctatttac
    40201 aaattgtttt tgtaaaactg aacaatatgt agtttcacaa caccatccta taaagctata
    40261 aattacaaaa taaagaaatt gaatataaaa tgtactcata agcacctcat ttctagttag
    40321 tataacacta tatactcgat attttatttt tttgattaat ttactaaatt caatttacat
    40381 attgattata ccaatttata gaaatttatc aaatttaatt acagttagaa cattataaac
    40441 atggttataa taatgaaatt ttatttaaaa taaataaggg tgtcaagtat aatcaaacta
    40501 atagttgtag atactctaca ctatgttagt ttaattacat ttgacaccct tatatttata
    40561 ctatgactat ttacattcga aactagagca acatgtttca atataaaggt ttactctatc
    40621 agtttcagga gcaaccaata tattatcaag tgtacataag ctaccatctt tgttgtattt
    40681 acaatttttt acagtacaaa ctaattcctc acaatttcta ttattaacgt tgctagataa
    40741 attactataa atagattcat ctaaaaatga actacaacaa gtatgtctgt cattgtgagc
    40801 tttttcacca tttataaaaa ttttatctgc ataacatata tggctgttgt tatgtgaaca
    40861 gttaattaca ttacagttta ttctttccaa agttattacc tcctaaacga ttatttatta
    40921 taaattatgt ccatataatt acaagaatat aaccattaag agaaaactac cttttagaaa
    40981 atatctattc ttagagtaat ttgaaatata aatatatttt cttctaaaat ttacctaaat
    41041 tgtataaaaa cttacagctt ggagaaaata atagaaaaag gaggcgtaat caatgtttga
    41101 aaagaagaaa aaagaattat ttccttggaa aatatctgcc ttaatactgg ttggagtatt
    41161 tgtattaagt agcggagttt atatagggac aagattaaaa cacaatggag ttcaaactgg
    41221 tgaagttgta agtactagtg cgaatatgaa tgaaaagtca aaagaagctt ttggattaaa
    41281 taaaaattgt gaagtttggc ttcaaagtga aggcgaaaat gaaactaact ataagagaac
    41341 acttacaatg gcaggaatag ttccagagtc attattagat aagacgaagt cagaaatagt
    41401 atcatatttt aaaaaagaat atccaactaa aaaaataaag agtatggata aaaatgaaat
    41461 catattggta gaatctaaat atagtgataa taaagatgta caagcaatgg atgttactat
    41521 aaaaggtaaa tatactattg aaaatgataa tggaaatata gcactatata aatatgatga
    41581 tagcggaagt aaaacattag tagaaaaaac aaaaataaga gtagattcac ttcctaaaac
    41641 agttcaagat gaaatcaaaa aaggtgtagt tatggatact gaagaagagg cctattcaag
    41701 attagaagac tttgcaagtt aatagtaaat taatattatt ttaagttaaa tactaagtaa
    41761 gttaaatact aagtaaatta ataataaata atttaatatt aaatagatta gtattaaaat
    41821 tgtagtcaca tttaattcct ataaaaatac aataatttat ttgtaataaa tataaaagtt
    41881 atattgaggt aaaatcttgc tttgatataa cttttttatt tgattttata ttttacatag
    41941 agcattttat gatttgttaa tattatttat gatataatga tttagaacat aagtttttat
    42001 gagaaataca ttttggtatt tcttttattt ttagggggaa ttttaaaaaa tgataatatt
    42061 ggggattgac ccaggtatag ccatagttgg atatggtata attgaataca aaaatagcaa
    42121 gtttaaagta atcgattatg gagcagttac aacacctgcc catatgaata tatcgagaag
    42181 attggaactt gtgtataaag gaattgatac aatagtaaag aattacaata tagatgaagt
    42241 tggaatggaa gaattattct ttaacaagaa tgtaaaaaca gctataacag ttgcacaagc
    42301 tagaggtgtt actatgcttg catgtgctca taatgggaag cctgtatatg aatacactcc
    42361 acttcaagta aaacaaggtg tagttggata tgggagagca gataaggcac aagttcaaca
    42421 gatggtaact tcatttttaa gtctaaaaaa agttccaaaa ccagatgatg ttgcagatgc
    42481 tttagctgtg gctatttgcc atgctcattc aaacaaactt gaaaaaactc taaagaatat
    42541 aggtggtaag tatgtatagt tatataaaag gtacagtaga agaaatttat atagacagta
    42601 tagttgttga aaataatggg ataggatata aaataaatgt atcatccaat acaataatga
    42661 atttacaagt gggagaagcc acaaaaatat atactaaatt aattgtaaga gaagatgata
    42721 tgagtctttg tggttttgta agtagggaag aacttaagat gtttgaatta ctaacatctg
    42781 tatctaaaat aggacctaaa gtggcgcttt caatactatc atttgcatca ccagcacagt
    42841 taggagcata tattttaagt gaagacattg gaaaattatc caaagctcct ggtgttggta
    42901 aaaagacagc agaaagaatc gtcttagagc ttaaggataa ggtagacaaa aataacatag
    42961 agtttgaacc aacgttatta tcacaaaaac ctaccttgat atcacaagat gagtctgtag
    43021 atgcacttgt agctttaggc tatactttgt cggaatcaaa agaggcagtt caaaaatgta
    43081 aaaaagatgg tatgaataca gaagctatta taaagaaagc acttacatat ataatgagta
    43141 aatccttgaa atagtatttg gattttataa tatttggatt ttaaaacaat atttaaggag
    43201 tttatgtaaa tttgaaaata tataaagtag attttcaaaa gtatgagagg aggggtataa
    43261 atgcaaggtt ttgaagatga aaataggata ataacttcta caatgaagat ggaagatata
    43321 gatatagaaa atagtttaag accaaaaact ttagaagatt atttgggtca agaaaaatcc
    43381 aaagaacaac ttagtatatt tatagaagca gctaaatcga gaaatgagca gttagaccat
    43441 gtgcttttat atggaccacc aggtcttgga aaaactacac tagcaagtat aatagcaaat
    43501 gagatggggg taaatttaag aattacgtca ggacccgcaa tagaaagagc tggagatttg
    43561 gcagccatac ttacgaattt aaatgaaaat gatgttttat ttatagatga gattcataga
    43621 atcaatagaa gtgttgaaga agttttatat ccagcaatgg aggatttttg cctagatata
    43681 ataataggga aagggccatc tgctagaagc ataagacttg accttcctaa atttacactt
    43741 attggagcta ctacaagagc tggtatgctt acaaatccac ttagagatag atttggtgtt
    43801 atttgtaaat tggactatta tacagttgat gaactttcaa aaatagtact taggtcttca
    43861 agcatcttag atgcagagat acagtctaat ggagcattag aacttgcaaa acgttcaaga
    43921 gggactccaa ggatagcaaa tagattacta aaaagagtta gagattttgc acaagtcaga
    43981 gctgatggaa aaataactga taaggtggca aaagatgctc tggaactttt aggagtcgat
    44041 agtttgggtc tcgattttgt agatgagaag cttcttatga ctataataga aaaatttaga
    44101 ggaggaccag tgggtttaga tacattggca gcttctatcg gagaagatag aaatacaata
    44161 gaagatgtat atgagccata tttgcttcaa ttaggattta taaatagagg tcctagagga
    44221 agagtagcta tgccattagc atatgaacac ttaaaaattc catatcctaa tgaaaaataa
    44281 aaatagatta tacgagtatt tataaaataa aagagtcatt aaattaaaat atttctaaaa
    44341 tggctaaaaa ttcaaacata tccacaaaat ggtaattata tctgtagata ctgtggataa
    44401 acacgataaa aattgtgtgt attgtatcta tatattgtgg ataagacatg tatatcgtgg
    44461 aaaaatatct atatattgtg gatatggtgt ctgttaaaaa tagcaatttc ttgatagatt
    44521 atggtaatat tgtggaaaat aagttgtttt tttgatatta ttgtagctgg agaaatttta
    44581 agagaggaag tttaacattg aaaacaagtg attttaaatt tgacttacca caggaactta
    44641 tagctcaagt tcctatagaa gatagagcta gctcaagatt gatggtatta gataaagaga
    44701 caggtaatat tgaacataaa gtatttagag atataataga atatttaaat ccaggagatt
    44761 gcttagtatt aaataatact agagttatac cagctagatt aataggagag aagcttgaaa
    44821 ctggaggtaa aatagagttt ttattattaa aaagaacaga ggaagatact tggcaggctt
    44881 tagtaaaacc aggaaaaaga gcaaaagtag ggactaaatt ttcttttgga aatggaaagc
    44941 taattggaga agttgtagat ttatcagatg aaggttcaag aattataaaa ttccattatg
    45001 atggaatttt tgaagaaata ttggatgaac ttggaaatat gccactacct ccatatatca
    45061 ctgctagact agatgaaaaa gaaagatacc agactgttta ttcaaagcat aatggttcag
    45121 cagcagcacc tacagcagga ttacatttta cagaagaatt gttaaataaa ataaaagaaa
    45181 aaggtgtaga tatagccttt gtaactctac atgttggctt aggaactttt agaccagtaa
    45241 aagtagagga tgttttaaat cacaagatgc attcagaata ttatatggtt agccaagaag
    45301 cagctgacaa aataaataga gcaaaggaaa atggaaaaaa tgtaatatgt gttggtacta
    45361 ccagttgtag aactatagaa tcagcttgta atgagtatgg aaagatgaaa gaaactagtg
    45421 gatggacaga aatatttata tatccaggtt ataaatttaa agttttagat aaacttatta
    45481 ctaattttca cttaccagag tctacactaa taatgttagt aagtgctatt tgtggaaagg
    45541 ataatgtttt aaatgcatat aatgaggcag taaaagaaag atatagattc ttcagttttg
    45601 gagatgctat gattataaag tagggaggta atggatgcat agtttacaaa aacaaaaaaa
    45661 gaataaccct atattaaaga ttgcttttat atttgaacgt gttttagcag tagtagttct
    45721 aatagcagta tttttaggaa ctatagatgt tctaagactt atgtggggag catatatagt
    45781 tgattttcaa aatccagtac aatacagtca gcttaatgat tttttagcac aaatattact
    45841 tttagtaata ggtgttgaac ttgtagtaat gctatctctt catataccag gagcatttat
    45901 tgaggcatta ctttacgcaa ttgctagaaa aatgttgcta ctgcctaaaa atgaaggtat
    45961 gatagatgtt ttaataggag ttatagctat agcaggacta tttgctataa aaaagttttt
    46021 agtaacaaaa gacaagagtg ctcttaatat aatgagtatt catgatgaag aagcaattaa
    46081 ggaacaacag aacaaagaag acgaagataa atctatttag acaataaatt atatgaatag
    46141 cttattacaa atatatctta agaaataatg agaaataaat atgattaagg agaactttat
    46201 gtacgcagta agatacgaat tgataaagac ttgtaagcag agtggtgcaa ggttaggaag
    46261 attacacact ccacatggga ttattgaaac accaatattc atgccagttg gaactcaagc
    46321 aactgtaaaa tctatgacac cagaagaatt aaaagagatt ggttcacaaa taatacttag
    46381 taacacatac catttatata tgagaccagg tcatgaactt ataaaaagag ctggtggtct
    46441 tcataagttt atgaattggg ataaaccaat acttacagat agtggagggt tccaggtatt
    46501 tagtctaggg cctttaagaa agataaaaga agaaggtgta gaatttaggt cacacttaga
    46561 tggttcaaaa cattttctaa ctccagaaaa agctatggaa atacaaaatg cattgggttc
    46621 agacataatg atggcatttg atgagtgtgc accataccca tcagatagag aatatgtaaa
    46681 aaattcttta gaaagaacaa caagatggtt aaaaagatgt aaagatgccc ataataatac
    46741 cgacaagcaa gctttatttg gtataataca aggtgggatg tataaagact taagggaaca
    46801 atcagcgaaa gaaataacta atattgattt accaggatat gctattggtg gacttagtgt
    46861 tggagaacca aaaccactta tgtatgacgt gttagagcat acaactccac ttatgccaaa
    46921 ggataaacca agatacctaa tgggagttgg tagtccagat gacttagtag aaggtgttat
    46981 aagaggagta gacatgtttg attgtgttct acctactcgt atcgctagaa atggaactgc
    47041 aatgactagc caaggtaaag tagtagttag aaatgcaact tatgctgaag actttactcc
    47101 acttgaccct gaatgtgatt gttatgcttg taaaaactat tcaagagctt atataagaca
    47161 ccttataaaa gcaaatgaaa tactgggtgc aagattaata actactcata atcttcactt
    47221 tttattaaat ttaatgaaac aaataagaca agcgataatg gaagatagat tacttgattt
    47281 tagaaatgaa ttttttgcaa aatatggata tgaaatatag gttttgcact attaattata
    47341 atcattttaa tatataatat tcaatagtac tcaaagatag agaaagtaaa ttgttatata
    47401 aacatttatt atgatttaca aaagagtata atttacaaag gagtgaaaaa ttaatgccac
    47461 agcaacaaat aattatgagt ataggtctat gggtagtagt aatcgcaatc ttttatttcc
    47521 taatgattag acctcaaaag aaaaaagaca aacaattaaa agaaatgaga agcagcctaa
    47581 gtgtaggaga caaggtcatt acaataggtg gaatagtagc taatgtagct aaagttgaag
    47641 atgatgttgt aatattagaa ttaggaccaa atagaactaa agttccattt gaaaaatggg
    47701 ctataggaac tgttaaatct aaaaaagaag aaatagaaga agactaaatt agtaatttaa
    47761 aagccttttc tcgaatatat attaatttga gaggaggttt ttttatggga aaatctaatt
    47821 atatttttaa aggtctagga tatgcatata taataacttt ggctgtttta ttagtgtata
    47881 acttattttt gacatttaca gatattggtg gagataatat aactatggtc tcatcattta
    47941 taactactat ttctgcagct ataggagggt tttacacttc aaaacacatg aaagaaaaag
    48001 gacttatgta tgggctttta gtaggacttt tgtacatagt atgtattttt ttgactgtgt
    48061 ttttagcaca agaaaagttt gtatttgaaa tagggatgat ttataagtta ttgctaatat
    48121 cagcagcagg aggcattgga ggcgtattgg gtgtcaactt taaatagagt ttatattaaa
    48181 gtagttttat gatataatca ataggaaaca attagaaatg gaggcgttga aaatggaaaa
    48241 taaacatgta aaaactttat ctcaagctac tttaaagcaa agtgctgcaa aaggtggatg
    48301 tggagagtgt caaacttctt gtcaatcagc ttgtaaaact tcttgtacag ttgcaaatca
    48361 agagtgtgaa agatagaaaa ttattgattt agttgtttgt aagagggtat ctcaaagtga
    48421 ttcttttatc attatgaggt atcctctttt attttcaatt attatataaa aatatatatt
    48481 ctatttatgt agaagtaaat ttgatttctt atcgtttaat tatgcaaaaa tatctaatca
    48541 tgtaagagca tttattttaa agtagctact cttatatttt attttttatc aattacatgt
    48601 attagaataa tttttaattt taaactctaa tttatgatga tttttataat atgttatata
    48661 ttttaatata tggagataga tattaattta gtgatatata tttaagtaat aagagtaaag
    48721 caaaaattag tgcatagtag tatataaaaa tggagtttga atacttttgt tagtatatga
    48781 ttgtttagta gaaaatcact ataaaatagg atttctatct ttgagcataa ttatagagag
    48841 cctaagtagg gggaagggtt tcaagaagat attattagtg tttgtgttag taaattatgt
    48901 aaacacataa aatatattga aaaataatat tatatataat attatttatg tgtgggaaat
    48961 acgaatataa aaaataaaaa ataatattcg gaggacataa atatgttaag caacaaaaag
    49021 agaagtatgg caatagtaat ggcaggtgct acagttatga gtgcagcagc accaatattc
    49081 gcagacaata ccgtgacaga aaatgttgat aaaaattaca cagtaagtgc gaaagattct
    49141 gctaaactaa tcgaagaggt tagaaaagca ttagaagtta agtttgaaga tacaaaagca
    49201 ggagcaaatg taaatgatag agtttacgat ataaaagtag acaatgtaaa cttaactaat
    49261 gcaactcaat tacaaaataa aataaactct ttaacagaag ggcaaagttt aaaagttact
    49321 attcaagata aagggcatca agtgttaggt ggaaaagtag ttgactataa aattgaaaac
    49381 tacaaaactg ctcaagaaat agtagatgca gttaatgctt acaatgcaac tttagcagaa
    49441 gattctgata acaagttaac agcaactata aaatctacaa atacagttga agtaaaaaga
    49501 gcaaaagatt cagcaaatgt tattacatta aatgtaggag accaacattt agatttctca
    49561 aaagtgataa catcagaaga aggtactttt gaaggttatg aaaagagtta tagtgacata
    49621 gattcaaaag aattacatac gataacagtt aaaaacgcag atttacaaga catatcagca
    49681 gaagaattat ttgatggaat tagattaact acattaggaa gagaaatagt taataaagtt
    49741 aaaaatggat atgctttaac atttgaaaat gaagcaatcc ttactcaaga acaagaagat
    49801 agtgatgata aagataaacc agaaaaatca agctttgata tagttttaag caaggcaaat
    49861 gaaaagccag aaacaataag tgtttcaagt aaaaaccata agttagttag agacttacac
    49921 aaagtattaa ctgatgtaaa agatggaaaa gaattaaaag tagaagtttt atctggtgat
    49981 tcaagattta caacagcagt agaagttagt aaagagagat ttaaagatgg agaagcagaa
    50041 gctataattt tagttggaga agatgctata gttgatggat tagcatcagc accacttgcc
    50101 tctcaaaaaa atgcaccaat attattatct aaaaaagatt cactaccatc agaaatagaa
    50161 gctgaaatat taagagtact tggaagtaat ctatcttcta agaaaatata tatagtaggt
    50221 ggagaatcta aagtatcaaa agaaactgaa gaaaaacttt ctaaattagg tgtaagtaaa
    50281 gttgagagag tttctggaga agatagattt gaaacttctt tagaaatagc aaaacaatta
    50341 aaagatacat ttaagactgc ttttgtagta ggtggaaatg gagaagctga tgctatgagt
    50401 atatcagcta gagctgctca atttggtgct ccaataatag ttacaggtaa cgaattagat
    50461 gcaaatgctg aaaaattatt aaaaggaaaa gaattagaaa tagtgggtgg agaaaattct
    50521 gtatcaaaag aagttgaaga caaattagtt gatatagatt taaataataa agttgaaaga
    50581 ttagctggag aaaatagaaa agatactaat gctaaagtaa tcaataaata ctatgcaggt
    50641 gcaactaaag catatgtagc aaaagatggt tatgtaggtg gaaatggaca attagttgat
    50701 gcacttacag cagcaccact tgcagctagt tcaaaggctc caatagtatt aactacagaa
    50761 gaactttcta aatcacaaga agaagtagtt gagttaagac ttaagaatgc tactaagtta
    50821 gtacaaatag gtgaaggaat agctaaaaac gctattgaaa aaatagcaga aaaaataaac
    50881 ttatttacta agaactaata agatatagga attaatatta aaagtttatt tagataatat
    50941 atgattatac aatgcgttaa tatgtaagac atagtgtaga tattataaaa tatacaaata
    51001 acaagataaa ataataaaaa taaggtgtct catttagaat tagattgaga taccttattt
    51061 tattttttga ttataatgtt gagaaattca tattgcaaaa attattataa aaagatactt
    51121 cttataatga ttaaacatat tactaacata aattattttg aacttataac gtaattaaat
    51181 aaaataaatt ataatgtttt gaatttatag agaataaaaa ctttaggtat attgtttgag
    51241 ttgtatggat aactatggta tcatatataa aatatggtaa caaaattgta acttttgatt
    51301 aaaaataagt atgagtacta ataaaaaata tgaaaagtac attgggggta gaaaataata
    51361 tgaagaatag taaaaagata ttagctatag gacttacact atttttagta atggtaaata
    51421 ctcctatggt aaatgcgttg acatcagttg agcaaataaa agggaatgac agatatgaga
    51481 cagcagcaaa aatagcagat aagcaaaact acaatacagc aatactaatc aactcagata
    51541 atagcttagc agatggtcta agtgcaagtg gtttagctgg agccttaaat gcgcctattt
    51601 tgatgacaaa acaaaaccag attccaaaca caactatgga aagattaaac aaagcaaaga
    51661 ctgtgtatat aataggttca gaatcaacaa taagtaaaaa tgttgagaat cagttactat
    51721 ccaaaaagaa ggttgtacaa agaatatttg gtgaaaatag atttgataca agtataaaaa
    51781 tagctgaaaa aatcaaagaa attaagccaa tagataaagt aattatagcc aatggattta
    51841 caggagaagc agatgcgata agtgcatcac cagtagctgc tagagatgga gtacctataa
    51901 tacttacaga tggaaatagt gtgggatttg acacgacagg cttaaagagt tatgcgcttg
    51961 gttctagtga aataataagc gacgaattag ttaagagcac aaattctatt agattgggtg
    52021 gcacagatag atttgaaact aataagatag ttatacaaga gttttataaa aatagtaaag
    52081 aattttatct aagtaaaggc ttacaattaa cagacgcttt ggctgcctct acaatagcaa
    52141 aaaatgctcc tgtagtttta gttgaaaatg gcagtaacaa gagtatttta agtggtgcag
    52201 acaaattgac tgtactagga ggcataaatc agaatgttat caagcaatgt ataaatcaag
    52261 cttcaccaaa tcaacaaggt ttatattaca atccaaatga tagagcattt aaagagagaa
    52321 taaagggtaa agtatatgct cttacaaagc agtatagaaa agagaatgga gtaagagcgt
    52381 tatctgtagc aagcagatta gagggtctag caaatgattg gtctaattta atggcaaata
    52441 agaagacttt atctcatacg ataaatggaa aaaattcata ttctactttt ttgaaatact
    52501 tagattggag tgagattaaa cctggatata tagcagttca aggtgaaaat ataattaaat
    52561 ataaaattcc agataaacct gtatatacaa atagagatgc agatgatata ggaaacttta
    52621 tttttaatga gtggaagaca aatccagaag aaggaactaa tatgttgcat aaagggtatg
    52681 aaataatggg atttggtata gctataacag gagataagaa tctatatgct acacatgaat
    52741 tttatggaag atataaagaa taagaaaata tacacaaaat tatgcttgta aggaagctat
    52801 ataaataatt aaattataat ggaaaatagc atttatatta ataaattaat ataaatgtta
    52861 tttttttata atttaatcta aaaaaattat attaaaaagt ttaaatatag tataaaatgg
    52921 gtaagaaagt agtgaaaatt aaatggcaaa attagggaaa tctaatgacg caaaactata
    52981 gggattaaag cttttaaagc catatcagcc agttgctaaa aagagtaaag tctttttatt
    53041 ttataattaa gattctcttt gagtgtcttt ttttattgtc tgaaacttat agtatttaaa
    53101 ttcatgaaaa attagatatt tattaaggga ggattgttat ttcatgaaaa aggcaatatc
    53161 ttgtgtacta gcagtatcta tgtgtagtgc accactaaac gtttttgcag aacctatatt
    53221 agaggggaag ctaagggcag ttgaaaaatc agttacagaa aagttaaggg ggagtcttga
    53281 agtagattta aatttttcac tgccaataaa aaatagcgag tcagaaagta tgactaatat
    53341 gcagttacga ttaaaagatg atagtaacaa tagtggaata ataaagcttg gagaaaaaga
    53401 ttcaggaaca gtaaatgttg ggacagataa tatagaatat actataaaaa aattatctgc
    53461 aagtagaagt gaaataaaaa ataaagatga gaatatatca tattataata ttgtatttaa
    53521 taatttacct gttggaaaat acaatgtaga agtctctgga gatggattta agaacaaaga
    53581 aattaaagac attgatatat caagttattc acaaagagtc ttattaagca atagagcaag
    53641 tgtagataat aaaaataaac ctttaaatta tgaattgttc ttaatgggtg atgtaaatgg
    53701 agacggtaaa gtagataaga gtgattataa taaggtttta gagaatatag attcaaataa
    53761 aagagaattt gatttaaata gagatggaaa ggtagatatt gttgacttag attatgtaca
    53821 gaaaaattta ggacaaagtg aagatgcaaa aagcctagaa agtatagtat ctaccaatcc
    53881 tatagtagat acaagtaagg ttgaattaaa aggaaatagt gatgtagata ttaatggtaa
    53941 tgtagaaaat ttatttagtg gagaaaataa tggagttaca atatcttcaa aaaatgatga
    54001 aatatctgag gaaaaaccag cagtaatgga tatagcttta tcagaagcac ttaatatgga
    54061 agtcatcaat ataaaagctc cagaaggtac tgctccagca aaaggaatag ttgaggttac
    54121 tgacaaagat ggtaatgttc aacagattcc ttatgaaaac aaagaatata tgaaaaactt
    54181 aaaagatgag gagcaacaag aggaagtaaa taacgaacaa gaagaagtaa atcaagaaga
    54241 agtaaataat aaagatgaaa atataaacca gactgataat aactcaaata ataaagatga
    54301 aaataatgat aaacaacagg aaaatagcga taaacaagaa gcaaactcta ataccaatca
    54361 agataaaaat aatgataaag aaaattcatc aatggataaa cctaattcag atgaagatac
    54421 tccaaaagaa gagggtaata tcagtaaaga tgataactct acaacatcta catatagtag
    54481 gtcagcatta gaaacatatg cagatgttca aaatagtgat atagtaataa atcttggtaa
    54541 acaagtagca gtaaaaaaga taactataaa aataacagct acaacagcaa acgatagaaa
    54601 tcttgcagaa atatcaaaag ttgaattttt aaacaatgta tataaagaaa taccaaagcc
    54661 agagatgaac atacctaaaa taaataaaat atcgactagc actgctgttg gaaatgaaaa
    54721 agtatctttt tattggaata atgaagtaaa tgtatcagca tacgaactta tactagacaa
    54781 aattgatagt aagggaaaag tattatctac taaaaaatta caaactagta aaaatacaat
    54841 agaagttaga gatatagaag catatggact atatagagtt agcatacaat ctttaaatgg
    54901 agattggtct agtggttata aagatgcaac accagaaaac ttagacggag taccagaaaa
    54961 tgtagattca aactataaac ctatacaatt tagcccagat agtatattag aatttcaagt
    55021 tgttccagac acaaaaccag aaccaccaga aggtataaat gttaaaggta aatttaaatc
    55081 tcttgatgta agctggaaaa atcataaaca agctaaagat tttgacttgt actataaaga
    55141 aaaaggctct aatgggtctt ggataaaata caatgataag cttattcgtg ggacaagttc
    55201 aaagatagaa ggtcttaagg ataatacaac atatgaagtc agaatgacag ctactaacca
    55261 cttgggaact agtggaatgt ctaaaacata tataggtaca actgaaagca tggatgcacc
    55321 aacaacacct aactatagat taattaatac tcctaaagaa aatggtggat ttggaatacc
    55381 aacaaatcac attgtagatg ttgtttatcc agatggatat gacaaaagtg aatatagtga
    55441 taaaaatcca tttgataaat tcaatgtagt agatggggac tttacaacac attggacatt
    55501 cccaacatat aatgcagctg agggaacgaa tagaggacca ataataactt ttgataaatc
    55561 ttatactatg gatactttta tggctacacc tcgttttgat gtttctgata agacaataaa
    55621 tgactttgag gtacgatatt gggatgaaaa tggtcaatta cacaatctgg gggatgttcc
    55681 ttttgaaaca aagacagacc ctgtaaataa taataagtat gtaatggtta gattaaaaga
    55741 accaataact gctaccaaga tacaagtaag tgtttgttca tcctattcat ataagtctaa
    55801 tatatcagaa cttaagtttt atgagtatga cagtatagaa gatgatgtaa gaaatctatt
    55861 tatggatggc ctacaagttg aattaaaaga agatgtaact caagaaaaaa taactgaatt
    55921 aaaagataga ttaaatactc cagataaagc tagtggagaa tatcatccat ataagacagt
    55981 tattgagaga gaattaaaat tagctcaaga cttatataat gatagagcca cacttactga
    56041 tgtaactact gtaaatcaag aaataaataa attaggaaaa tataaagata aaaacggaca
    56101 gtatactgta aattccaata atttaggtat gcaaaatgac tggcaggcat taggagtagc
    56161 tgctagagca ggagatgaaa tcacagtata tgttggaagt aaaagtggaa aaactcctaa
    56221 gttgatatat acacagtact atggagaatc tggtgcatat aagagtggag aaataaatct
    56281 taacactgga aaaaatgtaa ttcaattaag caaacttcat agtttagata tagaatgtgg
    56341 aggaagttta tacataagat acgctgaaga tactccaagt ggaggagata taaaagtacg
    56401 tgtagcagga gctactaaga taccacatct taatctaaat ggaacaataa atgataagag
    56461 tcctgcggga gtttctgaat ctaagaaaaa gataaaagca tatatagaag aattagctaa
    56521 atataaagat gctgtaaaag gagaaccata ttatccagga acaaatgttg gtaattcata
    56581 tggatattca gaaaaaactg gtgtgttaaa cactacagat attgaaagtg ataaagtaac
    56641 attaaatgtt cctgcaacag cagtatatca agagatatca aaaggaaatt ctgatttaaa
    56701 tacacaagta gatagattat ataatagtat gcttgcatgg gaacaaataa tggatttagt
    56761 ttactcagaa agaggagtat ttaaaacaca agatttagat ggtaatggaa ctgtagatga
    56821 taatgaattt aacttaacta aaaatgatat ggctccaaaa tctcgtatga acataaaata
    56881 ccaaagaatg tttattggag catttatgta tgcttctgga ttacatgtag gagtagaata
    56941 tggttcagtt ccagggctta tgaatggagt acctttccaa atagatgata gtggaaaagc
    57001 tacaggagga aacttatttg gatggggaat aggtcatgag ataggacatg ttacagatat
    57061 tggaaagatg acatatagcg aaactagtaa caatgtactt gctttacttg ctcagacctt
    57121 tgatgataag acacattcaa gacttgaagg ttcaacaatg gataagatat atgagcatgt
    57181 aacttcaaat agcttaggta taccaagcaa tgtatttgaa aggcttggaa tgttatggca
    57241 attacatcta gcttatgatg atgattttac gggttcaatg ttaaagaata actcagatgc
    57301 tgatttaagt aatgatacat tctatgcaaa gatttcaaga aaatacagag cactttcaag
    57361 tagtgaccca attaatagtt taccaaaaga ccaaatgcta gtagcaatgg catcaagtgt
    57421 tgttgaaaaa gatttaagag attactttaa ggcatgggga gtagaaatta caccagaatt
    57481 aaacagtata atggatagca agaactacga aaaagaatca agaaagatac aatatgtaaa
    57541 tgatgaagct agaagaaaga ttttaaatga aaatatagct agtatggcaa aagatactaa
    57601 agtaagtgct agtttttctg atggaataca aagtggttct ctagttaaat ctaataaaat
    57661 atctatagac ctagatgtag ataaggataa agataagata ctaggatacg aaataataag
    57721 aagtgatgga aattatttag atggtgaaaa tggaacacag gtaaaataca gaacagttgg
    57781 atttgtggat gcaaaaggaa atagcaagac taaatttact gataatatat ctcctcttaa
    57841 taacagagca tttacatata aggtagttgc atatgattat cacctaaatc caacagaaga
    57901 atttgaagtt ggtactgtaa aattatcaga tgaagggaaa ataaataaat cagcatggtc
    57961 attcataaca aatactgttt ctgacggtga tgttagaact gaaaatgact cacatggacc
    58021 attacaaaat cctgaaattg ataatataaa agataatgat gcaagtacta catataaagg
    58081 aaagataata agtaaagcag agtggaacaa agaccctcaa aaagaccctg atataaatgt
    58141 agaagaaaat ccatatataa tagtagacat gaaagaaaca ttacctatag taggactaaa
    58201 atatacaaaa ccagagacag aagctagaaa gttctcatta aaaggattat ttaactttag
    58261 aaagaatact agcacaacat acaatccttt aactaattac aagatacaag taagtaatga
    58321 taaaaagaac tgggaagatg taagtcaagg aacttttgaa tatggacaag atttagttgg
    58381 tggaggaaaa gaccaagata atgaagctag agttacattt aataaagatg gtaaactttg
    58441 gacataccaa gctagatatg taaaactaat ctcaaataat aagtctaata tagaagttgc
    58501 agaattagat ataataggac ctccaggtga taatatagaa attggcataa taaataatgc
    58561 agacgaaact cgtactaatg gaattggaaa attagcagaa gattatgtat atcaaagtga
    58621 tgactctagc acaagtgaag atgaaacagg taagattcca gcgggttcaa tagttattac
    58681 aggtgaatac agtggaaatc cagcatttaa tttaccacta cttatagata aaaacaataa
    58741 gactatatct ggagaagcac ttttatttgc agaagttcca gaaaaaggtg aactaggtga
    58801 aatcagtaaa ggtacatggg tttactatat gccaaaagaa aactttgatt ctttaagtga
    58861 taaggttaag gctgaactat atagatacaa tgaccttcaa ggagatactc cagtaggtca
    58921 aagatttgta agtgatacaa tgtatgtacc tgtaggagct aagaattatg atgagttaaa
    58981 gacaataagt cttactgata gtaatagtaa agctagaaaa gctacaagaa attctattga
    59041 tatcagtaat aagaaggtta taactgtaag caataaagta aagggtaata ttgttagaga
    59101 taaatagtag gtggaactct aaaaacgtaa aaatactaac attgtatatc taataagatt
    59161 aggtactaga atagttttaa aatagtagat attaaaagta aagtatataa atgtaaccat
    59221 aaatttaggg taatttataa attaaatata aaagctaggt ggataagtaa atttatccac
    59281 ttagttaatg atagataagg acgggtgtta atgagtaaaa gaaaatcttt tataagaact
    59341 atagcagtat caactatggc agtagctgta actggtagtg ctacatgtgc atatgcagct
    59401 ccagttttac aaggaacaaa aacgtatgag aaagtaaata ctatagatat atctgtggac
    59461 tcagtagaaa acatagtata ttcatttcaa gccagtataa aagtacaagg tgaggttgaa
    59521 gtagtagata atgagcagaa agaaaagatt acttggtctg ataatataaa atctcagata
    59581 aaaagtggaa atgcaaatgc tacttgtaga gtagaataca acaaatcaag taatacaact
    59641 acattagata tatatgttac atcaaatgaa gatttactag acggaaatag attgaatata
    59701 ggaagaatat ctgtaaaaaa atctggcagt aactcaaatg ctgactataa agttttagga
    59761 aaaggaacaa atgataaacc agctttaaaa attgtaacat ataacaacaa aacagtggat
    59821 tatgaaaata taagctctga tgaaggatta atatttaccc ttattaatga gagtgaagta
    59881 aaaccaattg gtggaactgg ttctagtaaa aatgaccctg aaaagtataa ggtagagaaa
    59941 agtgaagcac ttgaatattt attaaacaat attaggataa actattctat tgttagtaaa
    60001 gaaacacaag aaagtggttc aaatgtaata ttaaaacttg gattagcaca aaaaacaact
    60061 aagggaagaa aagctacaat aaacaaatat gttgaagtta cacttccaaa aagtttagaa
    60121 tacatagttg aaaatgaatt atcaaaaccg gatgaattac caccagataa tggcaatggt
    60181 ggaaataatg gtggaggaag taattcaggc ggctcatcta gtggtggaag ctctggagga
    60241 ggaagttcaa gtgattctac atctaatgtc acagtcaaaa aattaaaagg tgctgataga
    60301 tttgagacag ctatcaaaat atctcaaagt ggttggacaa aatcagatac agttgtaata
    60361 gtaaatggag aagataaaag tatggtggat gggcttacag ctacaccact tgctagtgta
    60421 aaaaactcac caattctttt atcatcaaat gaaaagcttc ctcaaaaaac tgtagaagaa
    60481 ttgaaaaggc taaatccatc aaaagtaatt gtgataggtg gaaacaactc aatgccaaat
    60541 tcagttgttg aagctatcaa agctgtaaat tcaaaaatat cagtacaaag aataggtgga
    60601 gatacaagat atcaaacttc tattaatata gcaaaagaaa tagataagac gaataatgta
    60661 agcaagttgt atataggagc aggaaatgga gaagctgatt cattatcaat agcatcactg
    60721 gctggtaaag aaaaaactcc aatagtactt acacaaaaag atggtgtaga taatgaagct
    60781 gaacaattta ttaaatcaaa taaggtgtca aatatatatt ttattggtgg agtagaaaaa
    60841 atatctaaca aggctattga acaagttgga aaaatagcaa acaaagatat atcaaacaat
    60901 agggtggcag gtcaaactag acaagagacg aatgccaaag taatagataa attctactct
    60961 caatctaagc tagatggagt tgtagttgca aatcaagata aacttataga tgctttagca
    61021 gttggaccat tagcagctaa aaacaattca ccagttatac ttgctacaaa tacgttagat
    61081 aaatcacaag aatcgtcatt aaaaggtaaa aattcatcaa agttgtttga agtaggtgga
    61141 ggaatagcct catctgtaat agacaagatt aagagtttaa ttgaaaaata aatattaagt
    61201 aaatagaaaa taagaatgta tttagatata aagttatata tttaaatgca ttcttatttt
    61261 attttcatta tcttaacaat ctaataatat tgaggttaaa atgtataata atttgagcta
    61321 tattaagttt aattttaaat aaagcagata tcttttatat atataaatat aaattcaatt
    61381 ttgaatatgt ttaaaaagaa gttaaaaata attttttaaa tttaataaaa taaaacacta
    61441 atatatagac gagatatatt catatcatat atactctata atatatggaa aataaagaga
    61501 aaataaatgg aaaataatca caatataaat ataaaatata aaaatcatca gggggatatg
    61561 aaaatgaaca aaaaaatatt atcattaggt ctagcagtat cattaatctt agtaaacttc
    61621 aaaagtgtaa atgcatcatc agtagtagaa aaaatatacg gtaaagatag atatgaaaca
    61681 gcagcaaaga tagctgataa acagacttat gaaacagtaa ttttagtaaa tacagaaaaa
    61741 tcacttgcag atggattaag tgcaagtgga ttatcgggag ctacaaaagc tccaatacta
    61801 tttacacaac aaaataagat accagcagac acaaatagat gtctaaaaaa tatcaaaaaa
    61861 gcatatataa taggtacaga agatactata agtaaatcag tagaaaaaga actagattct
    61921 aaaaatatag aagtcaaaag aattggtgga gaagatagac taaaaacaag ctatttaata
    61981 gctaaagaaa tagcaactat aaaaaaagtg gataaagtac tattaactaa tgcatatagt
    62041 ggagaagcag atgcaatgag tgtatcatca gtagctacta gagatggagc tccaatcata
    62101 cttacagatg gaaagagtgt gccttttgat gtaaaaaata ttcaatcata ttgtataggt
    62161 tcagaggaga taatgagtaa tcctctagtt aaaaatacaa actcagtaag aatagaagga
    62221 actgaccgat ttgaaactaa caaaaacgta atagactact tttttaatag tgcagatgga
    62281 ttctatgtat cagatggata ccaattagta gatgcaatag cagctgcacc acttactaaa
    62341 aactctccaa tggtactagt aaatgatgga agcgataaaa ctgtattaga aggagctaag
    62401 aatataacct ctgtaggtga aataaatgag aaggtaatac aacagtgtat aaatgcttca
    62461 aattcaaatg ggcaaccccc aacaattaca gttggaagta cagaggtata taagggtgaa
    62521 aagtttgaca ctggcaagtt aaatatagta gctaaagata acacagggaa ggtattacca
    62581 atagaagttg atgggtttat agatacaaat agagtaggta catacatatt gacattaaaa
    62641 gctacagacg aatggggaaa aagcacagga aaaagagtag aaataaaagt gttggatgac
    62701 aaatctcatg attacaacag cccagaattt aaaaaaatgg tatctactga aatgtataac
    62761 ttaatcaatt cctatagaaa agaaaaaggc aaagagcctt tgctagtgtc tagtaggcta
    62821 gaaggtatgg caaatgcatg gtctaagtat atgatggata aaaaagtatt tgcacattat
    62881 atagatggaa aaaatgctcc acaagtattt tctgaatttg gaatgagaag tgaggaaaat
    62941 atagcatata tttatataga ttctaagaac gttcaaacga cacaagatgc aaaagacttg
    63001 gcaaaggcta tatttgaggt gtggaagaag tctccagagt acaatgcaaa catgcttagt
    63061 aatgagtttt atagtactgg atttgggctt tatatattat ctgatggtca agtgcatgct
    63121 actcaggagt ttttaaatgg aaatgaaggt agcttgtaaa aaattccata aatcttgttt
    63181 ttttgttaat ttactgatat tataaaatga aaatcacatc taaagtactt tttgtaatct
    63241 gttttgttat tgctttacag gctatggtat tttaggtgtt ttttgtttta gtagtttata
    63301 gattttgagg gaatcactac tgaggatgta atttatgatt agctgaatta tataaaaaaa
    63361 ttagtaggag gagtttgatg aaggtaaata agagagtatt atcaatagga ttgacaatat
    63421 cattaataat ggcaggagca ccaaatatca atgcactttc gagcatagaa aagatacagg
    63481 ggaaagatag atatgagact tctgcattga tagcagataa acaaatatat gatacaatca
    63541 tattagtaaa tactgataat tctatagtag atggattaag tgcaagtgga ttatctggag
    63601 ttgcaaaagc tcctataatg ttggtgcaaa gagataagat accaacagat gttgaaaaaa
    63661 gactaaagga tgttaagaat gcttatgtta taggtacaga ggatacaata ggtaaatctg
    63721 tagaaaatca acttaagaat aaagggattg aagttaaaag aattggtggg gaagatagaa
    63781 taaagactag ctatcttata gctaaggaga tttcagctat taaaccagta aatgatggtg
    63841 ataaggtttt tcttgtaaat ggatacacag gtgaggctga tgcaatgagt gtatcttctg
    63901 tagctgctag agatggagtt cctgtgatac ttactgatgg aaagagtata ccatttaaag
    63961 ttgatggcgt tcagtgctat tctcttgggt cagaggagat aatgagtaat gaacttgtga
    64021 gtaagactaa ttcagttagg attgctggta aggataggtt tgaaactaat aagaaggtta
    64081 tacaacgctt ttataaagga acaaagaagt tttatgtttc tcaagggtat aagttagttg
    64141 atgctgtggc tggttctccg ttggctaagg acaaacctat agtacttgtt aatgatggaa
    64201 gtgataagag tgtgcttaga ggtgctgatg aagttacttc tttaggtgga atggataaga
    64261 aggttgttga ccaatgtatt agttctgcat ctgataaaaa tacaatgcct acaattactg
    64321 ctaatgatgt agagatttct gtaggggata agtttgataa tagtatgttg aatatagttg
    64381 ctactgatta ttatggaaat gatttaaagg caaatattaa aggaaatgtt gatattaaca
    64441 aggctggaac atatgtttta aatataagtg tagtggataa tttaggtcag aagagtgaga
    64501 ttagtgtaaa tgttaaggta gttgtaaatg cgagtacaaa agactctaat agttatgagt
    64561 ttaaggctat ggtatctaat gagatgtatg atttagttaa ttcatataga aaagaaaaag
    64621 gcaagaagag tttaagagag ttagactctt tagctggaat ggcaaatgca tggtctaaat
    64681 atatggaaga taagaaggta tttgcccatg agattgatgg taaaaatgca gctgaagtat
    64741 tttttggctt tggagcaaga agtggtgaaa atattgcata tctaccaatg aatgttaaat
    64801 ctgtttatac ttctaaagat gcaaaagaaa tggctgagtc tatttttgat ttatggaaga
    64861 agtcttctaa gtataatgaa aatatgttaa aagaagaatt ttatagcttt ggatttggta
    64921 tgcatgtttc tagtaaagga gaagtgaatg cgacaatgga atttttaaat agttagattt
    64981 tatattaatt tacattggaa aagatatagt aaatttgtaa aaataaatat ataaaattaa
    65041 aataacttat aaaattataa aaaataattt aaaaataata aaaatgatga aaaaacaatt
    65101 aatttgttgg aaaaaacttc aattaatttt gcataaatta ttgaatttat attttatata
    65161 gtctatcata agagtgtgag aaatatttgg attatatgaa catggaaatt taatgtacat
    65221 aattaaagag atgtaattaa tataatgttg ggaggaattt aagaaatgaa taagaaaaat
    65281 ttagcaatgg caatggcagc tgttactgtt gtaggttctg ctgctccgat atttgctgat
    65341 actactgtta aagaagaagg atatacggta gtacaagaca aatatgaaaa attacttaaa
    65401 gaattaaagg caaagataaa agatggaact attacatcag ttggtgttga atttgatgga
    65461 aaaccaatta ctacattagc accaaaggca gatggtagtg ataaagatgc aatagcagaa
    65521 cagttagaaa ctcttactaa aaaccaactt aaaggtttag gtgatggaaa atatgtagat
    65581 tttaaaatta cttatggtgc taaagcagaa gttccagctg cttcattaag tgcagatgat
    65641 atacaaaaat atgcagatca aataaatgct tctgagaaaa tattagttga agtagcagca
    65701 ggatctgaag caggtatagc aaaatttgat tctgttaaca ataaagttat agcaggagat
    65761 gctcctctta aagttaaaga tgcagtgaaa gcgactgtta ctactaatgg tagtaataaa
    65821 aaagttctta caatatcagc tgcagctgga ttatcaggat tttcatatgg aacacttaaa
    65881 gacacaggtg caagtttatc tgatgttgat gcaataactc ttgacactac aaatgcaaca
    65941 ataacagaag gtgatactaa agtattagat tttgataatt catttaagtt taacgaaagt
    66001 actaaaaaag ttggtagttt agttacacca aatacaacaa acactccagc agatcctggt
    66061 actaaaacta ctgttagagt gataaaagct gttgaaaaaa caatagatgt aagttcaaat
    66121 tcaactacaa aagctaaaga tttagctaaa caatatgtat tcacagatgt tagtgatact
    66181 gatccagaaa gtttaagtta tatgcttaaa aacataaatg atggaaaagt tgctgttaaa
    66241 aattctgatg gagattatga agtaactata ttcccagaag gaaaaagatt aaatacttta
    66301 tctgcttctt cagctaaaac tattttagga gataaagata ctccagcaaa aatagtttta
    66361 aaggcttcaa caacaaagaa attagcagac tatatagatg atttaataac ttataacaat
    66421 agttattcaa atgtacaaac tgtagcaggt agtgatagaa tagagactgc tatagagcta
    66481 agccgtaagt actataactc tactgacaaa aatgctttat atggagatcc tgtaaataac
    66541 gttgtattag taggttctca agctatagtt gatggattag tagcatctcc tttagcagca
    66601 gaaaaagatg ctcctttatt gttaagttct aaagataaat tagattcatc aacaagagct
    66661 gagataaaaa gagttatgga cttaaattct tcaacaggaa taaaaaataa taaagaagtt
    66721 ttcatagcag gtggagtaaa ttctatatct aaagacgttg aaaatgaact aaaagatatg
    66781 ggacttaaag taactagatt atcaggtgat gatagatatg aaacttcatt agcgatagct
    66841 gatgaaatag atataaatga taaagcttat gtggtaggtg gaacaggatt agcagatgct
    66901 atgagtattg ctccagttgc atctcaaata aaagatggtg aagctactcc aatagtagtt
    66961 gtagatggaa aatctgacaa gttatctaaa gaagctgaag atttcttaga tgatgcacaa
    67021 gtagatataa tcggtggaga aaatagtgtt tctgctaaaa tggaagatta tatagatgat
    67081 gctactggaa aatctccaga aagaattagt ggtgctgata gacaagcaac taatgctgaa
    67141 gttataaaag aatactttga taaagatgga gtttcaaatt acttcttagc aaaagatggt
    67201 tcaactaaag aggatcagtt agttgatgca ttagcagcag cagcagtagc tggaaactac
    67261 ggttctaagc ataatgaaga tggagatata actacagatg cttctcctgc tccaattatt
    67321 ttagcaactg ataatttatc agctgaacaa catgtagctg taagtaaaac tgctactact
    67381 aatggtgcta agaacttagt acaagtaggt caaggaatag ctgattcagt tgttagcaaa
    67441 ttaaaagatt tattagatat gtaatataaa ttttatatat tgaataaaaa aggtttctct
    67501 tttgagaaac cttttttatt ttaaacataa atttttataa ataaaaagag gctaattagc
    67561 ctctttttaa tgtgagaaac acttaaaaaa taagatgtaa ttatttattt aagtatcatt
    67621 attatactat accttaaaat aaatgtctac tattctataa ttttaaggta taatagtaag
    67681 tggaaaataa ataaggaggt aaattaaata tgtcagtttt agatgtaata ttagacaaaa
    67741 cagatgaatt agaaattaaa aaattaaata atatagtaga taaaatagat gctttagaag
    67801 ataaaataca aatactagat gatgaagcat taaaaaatat gacgaatgta tttaaggata
    67861 agttaagtaa aggagaaacc ttagatgaca tattgccaga agcatttgct gttgtaagag
    67921 aagtatcaaa aagaatatta ggtatgcgtc aatatagagt tcaattaatt ggtgggattg
    67981 tattacatca agggaaaata gcagagatga gaactggaga aggaaagact ctagtaggag
    68041 tagctccagt ttacttgaat gcattgactg gtaagggggt acatgtcatc acggttaatg
    68101 attacttggc tcaacgtgat aaagaactta tgcaaccagt atatgaattt ttaggtttaa
    68161 cagtaggagt aattattaat gggcaacagc cagtagaacg aaaattacaa tatcaatgtg
    68221 atattactta tggtacaaac aatgagtatg gattcgatta tttaagagat aatatggtaa
    68281 cgcaaaaatc agagaaagtt caaagagaac taaactttgc tatagttgat gaggttgatt
    68341 caatacttat agatgaagct agaacaccac ttataattgc tggtggagga gatgaaggta
    68401 ctaagctgta tgaacttgca aatgctttta taaagaatat aagagatgaa gattttgaaa
    68461 tggatagaaa agataaaacc atagcactta ctgcaagtgg tcttagtaaa gctgagaaat
    68521 tttttggaat agaaaatata actcatatag ataatataga aatttttcac catgtaaatc
    68581 aatctctaag agcacataaa ttaatgaaca tagatgttga atatgttgtt agagataatg
    68641 agatactaat tgtagatgaa tttacaggaa gaataatgga tggaagaagg tttactgatg
    68701 ggcttcatca agcaattgaa gctaaagagg gagtaaaaat aaatgatgaa tcaaagacca
    68761 tggcaacagt aacatttcaa aatttcttta gattatactc taaattatca ggaatgacag
    68821 gtacagcaaa gacagaggaa agtgagtttg aagctacata taagattaat gttgttcaga
    68881 taccaactaa taaaccagtt ataagagctg atttacatga taaagtattc aaaacagaac
    68941 gtgcaaagta caatgctgtt gtagaagaaa taataaggat aaataaaact aagcaaccag
    69001 tgcttgtagg aactgtttca attgaaaaat ctgagaaatt atctaagcta cttaaaaaga
    69061 aaggtgtaaa gcacgaggtc ttaaatgcta aacaacatga taaggaagct gaaataatat
    69121 ctaaagctgg taaattaggt tctgttacaa ttgctactaa tatggcaggt agaggaactg
    69181 atatatcact tggagctgga gataaagaag aagaagaaaa ggtgaaagag ttaggtggct
    69241 tgtatgttat aggtactgaa agacatgaga caagaagaat tgataatcag cttagagggc
    69301 gttcgggaag acaaggtgac cctggaactt ctagattctt tgtaagttta gaggatgaag
    69361 taataaaatt gtatggtgga aaaagtatag aaaaaatatt aaagagaatg aatgctaatg
    69421 aagatgacgc gttagaaagt aaggctttga atagagcaat agaaagagca cagaaaggca
    69481 ttgaaggtaa aaattttgaa caaagaaaga atgttcttaa gtacgatgat actattaatg
    69541 agcaaagaaa agtaatatat gatgaaagaa ataaggtatt ggatggttct gatattgaag
    69601 aagagataca aaaaatggtt atggatatta ttacggaggc tgctgaaaag tacttaaaaa
    69661 aatttagaga ctatcatgga tattttaagt atttatataa tacttttatg ccagctgata
    69721 ctttattaat tccagactta gataaaaaaa atgttgaaga aatagtaaat caaacatatc
    69781 agatttcaaa gcgtgtttat gatcttaaga agatgatgat tggaattgaa gaattggctc
    69841 aattagagaa acgagtatta cttcaagttg tggatactta ttgggttgac catatagatg
    69901 caatggatca actaagacaa tatataggtc ttaaatctta tgcacaaaaa gatccattta
    69961 aagagtatgc tatagaaggt tatgaaatgt ttgaagcttt aaataggaat ataagaataa
    70021 caacagtgca atatctatat aaatttaact aaaaaagtga cccattgggt cactttttat
    70081 atattaagat taaagtgaaa tttattttac agtacacact catatagata aatatttata
    70141 ataatgaaaa agaggctgaa cgtcagcctc ttttcctgtg tgagaaatac ggaaaaaaga
    70201 gatgcttgaa atttctttct tccatttaat attatactat aaagagaata aaaagtccat
    70261 gagttatctt tatgattaaa aaaaaaaatt acaattttgt tactttttag tatacaataa
    70321 taaatttaaa ttgactttgt taatttttat taataaaata tccaaatatt acaatatcta
    70381 cagtttattg aaataatgtg taaattatat tataattggt aaataaggaa aaatggtgaa
    70441 aaccaaattt tgtaggggga aatatatgaa tagaaaaaat ctttatgtaa ttatggcagc
    70501 tgcaatggta agtacatcag tagctccaag ttttgctgca gaagctacac aagtaaagaa
    70561 acaaacaata actaaaaagg aagcaacaga attagtttct aaagttagag aactaatggc
    70621 tcagagatat acaggaggtt ctcaagttgg acaaccaata tatgaaataa aagttggtga
    70681 aacttcatca caattaaaaa taataactaa tatagacgaa ttagagaaat tagtaaatgc
    70741 tttaggagaa aataaagagc ttcttgtgac tatagcagat aaaggacata caacaaatag
    70801 tgcaaatgaa gtggttgctg aagcaattga aaaatatgaa aattcagcag acctttcagc
    70861 tgaagctaat tcaataacag aaaaagctaa aactgaagct actgggattt atggagtggc
    70921 aactgtagaa gcttcatatg acagtacaaa agataagtta gtaataactt taaaagataa
    70981 gactgatgta gtaacttcta aaacgctaca aataggtatt ggtgatgaaa aggttgattt
    71041 aacagcaaat ccagttgata tagcaggaac caatttagac ccttctgcag aaggatttag
    71101 agtaagtaaa ataaataaat taggtgtaga aggagctaaa aatattgctg atattcaatt
    71161 agctgaaata actataaaaa atagtgattt aaatacagtt tcacctcaag atttatatga
    71221 tggatataga ttgactattc gaggtaatac aatagcaaat agtgtaggaa aatcaattag
    71281 tgatatttca tcaaaagatt cagagacagg aaagtataaa tttactgtta aatatactga
    71341 tacatctgga aaagcgatag aacttactgt agaaagtact aatgataaag agttaaaaga
    71401 tgttaaagat gccttaaatg gaaatacaaa agtcaattta atagctgggg aagacaggta
    71461 tgcaactgct gtagctatag caaaagaaac agaatatact gacaatatag ttatagttaa
    71521 ttcaaataaa ttagttgatg gattagcagc aacaccacta gctcaatcta aaaaagctcc
    71581 tatattgttg gcgtctgata atgaaatacc aaaagtaact ttagattata taaaggaaat
    71641 aattaaaaaa agtccatcag ctaaaatata tatagtaggt ggagaatcag cagtatcaaa
    71701 tacagctaga aagcaattag aatcagtaac taagaatgtt gaaagattgg ctggagatga
    71761 tagacataca acttcagtag cagtagcaaa agctatgggt tcttttaaag aagcatttgt
    71821 agtaggtgca aaaggggaag ctgatgctat gagtatagct gctaaagctg ctgaacttaa
    71881 ggctcctata atagtaaatg gttggaatga tcttacagca gatgctatca agttgatgga
    71941 tggaaaagag attggtatag ttggtggttc taataatgta cctagccaaa ttgaaaatca
    72001 acttgctgat attgataaag atagaaaggt tcaaagagtt gaaggtgaaa caagacacga
    72061 tactaatgct aaagttatag aaacatatta tggcaaatta gataaactat atatagcaaa
    72121 agatggatat ggaaataatg gtatgttagt agatgcattg gcagcaggtc ctctagcagc
    72181 aggaaaagga ccaattcttt tagctaaaaa tgatataaca aactcacaaa agaatacttt
    72241 agataaaaaa ctaaatcttg gtgcagaagt aactcaaata ggtaatgggg ttgaattgac
    72301 tgtagttcaa aaaataggca aaatactagg atggtaataa tcaataatat agaaaaggat
    72361 tccttattat ttataggaat ctttttctat cgacataata ttcattaaag tataacatgc
    72421 tcttgacaga taggaggata tgatagtgat agaagcaaca ttgttttttt ccccacacca
    72481 agatgatgaa acattaagta tgggtagtgc aataattgaa catgtagaaa gaagcgatac
    72541 acatgttata ttatgtactg atggcagtaa atcaataata agaaaagtat tagatgatgg
    72601 agggtcatgt tcttatcata ttaaagatgt acacaagtac tctttatcag aaagtgattt
    72661 ttcaaaaaat agagatgaag aatttaaaaa tagttgtaaa gcaatgggag ttaaagaaaa
    72721 taatatacat atagaaaata atagagctca tgatggagaa ttgagtaagg aaaaagcaag
    72781 agatataatg ctaaaatatt tagaaaaata tcctgatgct aaagtgaaga ccgttacacc
    72841 atataaagct cttggaatac atgaagacca tagagcatta ggtgaagctg cattagaatt
    72901 gtatagagaa ggaaaaataa aagatttaag attttatgta gagccatacg attataatga
    72961 ttttaaaaaa gttaatccaa atgttagagt atggaaggtt ttgagtagtc aagaagagaa
    73021 attattaaat gctatggatg catacaagaa atggaatcct gaaaatggta actacgcaat
    73081 tggatatcat tctgtaaaat ctcactttga tgaattaaaa tccaataaaa tccaatatgt
    73141 acatgctcca taaacattaa tataaaaact aggaggatat agttaatgaa aatatctaag
    73201 agaataatgt cgttgttgac agcaactttt tttatctttg cattatctat caatatagct
    73261 gatgcttcat caaaagagag tctagtgggc tctggtagat gggaaactgc aataaaaatt
    73321 agtcagaatg gatggaaaga tgctactaat gctatactag taaatgataa ttctatagct
    73381 gatgcattat ctgcaactcc atttgctaag ttaaaagatg cacctatttt attaactcaa
    73441 agtgataaat tggatagtag aactaaagca gaattaaaaa gactaggtgt aaaaaatgta
    73501 tatttaattg gaggtatgaa tactctaagc accaatattg aaaaacaatt aaaatcagaa
    73561 aatatgactt ttgagaggat ttcaggtaat agtagatatg aaacatcttt aaaattagct
    73621 gaaagattag ataaagagaa gagtatttct gatatagttg tagttaatgg agagaaaggg
    73681 ctagcagatg cagtaagtgt gggttctgct gcagcacaag aaaatatgcc aattatactt
    73741 tcagatccta acaatggaat aaaagttgca aatagcttta ttagtagtaa aaatataaag
    73801 aaatcttata taattggtgg tacatattct gtatctagtt ctattgaaca gagtttacct
    73861 aatgtaaaaa gattatctgg aaacaataga aatgaaacta atgcaaaggt tctagaagaa
    73921 ttttataaaa atacagactt aaaaaatgct tatataacta aagatggtat gaaaaaacag
    73981 ggggatttga tagattctct agctgtaggt gtattggcag ctaaaaatag ctctccagtt
    74041 gtattagtag gaaattcact tgactctagc caatatgatg tgttaaatac taaaagtttt
    74101 gataatattg tgcaagttgg tggaaatgga aatgaagatg ctgtaaagga tattcttgat
    74161 ttacaagaag aaactatata tagagttaag acagcagacg aattggatgt tgctctaaga
    74221 aaagcagatg ctaatgatat tataacattt aaacctgatg ataataaaaa aattactagt
    74281 tcatttagta tagaaacaaa gaaagctata actgtagagc ttgaaggatt gtatacacaa
    74341 acaataactg taaatatgcc aaatggagaa ttaactaata agggtacatt agatggaact
    74401 gtgaaaataa tggatataaa aaatggcaca tttatcaatg aaggtgatat aaatgatatt
    74461 gacatttatg atccaaatgg atgtaaaata gagaataaaa gtggtggaga tatatgggtt
    74521 ttaactatat ctagtagtgc taaagatgta aatatagaaa ataaaggtga tataagtaag
    74581 gttacaaata attctactag cgttataata agaaattcag gaaatattga tactttaagt
    74641 ggaattaatg caccagcaat aagtggaaat aaacctagag ttgatgatac cgataaggat
    74701 gaaaaaagag caagtggaat atatcctact tcagaacctt tatcacctgc aagagctggt
    74761 tatataagtg taaaaatatt cactaagcct gaagatacaa attataaaat ttattataga
    74821 gtagtaagca gtagaccatc tgcattatat atcggagata gaatagatac aagtgactgg
    74881 actgctgtac catcttctgt tttaccattt actattaaag cagaaaatgg atattatata
    74941 gaagctgttg aaataaattc tgatactaag aaagtatcta gatgggggaa atctgactat
    75001 atagatgatg gattttattt aaaatcagaa actgcaaaag gattaagtgc agatatagat
    75061 tttgatggag attatgttaa attaacagca acaggtgctg attctggatg tacagtttat
    75121 tatagaatag tatcaaaaaa gccagtatca atgaatgttg gagataaagt aactatatct
    75181 gattgggatg aaattactac taatagagtg aggatagaag caaacgatgt aaaggataaa
    75241 tatatagaac tagttgaatt ggatgattca actaataaag taactagatg gggagcaagt
    75301 gataaggtag tagaataggt atctaatgtg aaacataaaa acagactgat taataatggg
    75361 cagtctgttt ttatttccta aaaattatca ttttactaaa taaataattt aaaactataa
    75421 caattatatt agtaaatact tttacagtaa tatcatttac aaaaaataaa tcaatcatta
    75481 tatacataat aatcatttct ataataccag aaagtagcct aaaactgaca aaaaacaaaa
    75541 actccttaaa caaaaatatt agttcaattc ttttactctc aaacacaaaa aacttattag
    75601 taacataagc aaataaaaca gcaagtatcc atgccaaagc atttgcaacc ataaaattaa
    75661 ataaaataac tctagtaaaa aataaatacg aaactatatt tacaagagta gtaaatgctc
    75721 caaaaaataa atataatatc gtctctctat gcttttttaa aatcaaatat aatcctccta
    75781 agctaattta atataaatat acttaaagat actaacaaac attagtataa cacatctatt
    75841 aataaatcaa tatatcttaa aatattataa aaataaaact ataatattct ataattaata
    75901 tgtactcata atccatattt tatcaaaata aaatatgacc ttaatttaga aatctgatat
    75961 aataaaaatt aaatacatat aagggggtaa acatgagaaa gtacaaatca aaaaacttgt
    76021 caaagctact agcattgttg acagtttgct ttttaatagt gtcaacaata cctgtctcag
    76081 cagaaaacca taaaactcta gatggagtag aaactgcaga gtattctgaa agctatcttc
    76141 aatacctaga agatgtcaaa aatggagaca cagcaaaata taatggagta ataccttttc
    76201 cacatgaaat ggaaggtaca acacttcgaa ataagggtag aagtagtctt ccatcagcat
    76261 ataaatcaag tgtagcatac aacccaatga atcttggtct tactacacca gcaaaaaatc
    76321 aaggaagtct taatacatgc tggtcttttt caggtatgtc aacgttagaa gcatatctta
    76381 aattaaaagg atatggaacg tatgacttat cagaagaaca tttaagatgg tgggcaactg
    76441 gtggaaaata tggatggaac ttagatgata tgtcagggag ttcaaatgta acagctatag
    76501 gatatctgac agcatgggca ggtcctaagt tagaaaagga cataccatat aatcttaaat
    76561 ctgaggcaca aggtgcaact aaaccttcaa atatggatac tgcacctact caatttaatg
    76621 taacagatgt tgttcgtctt aataaagata aggaaactgt aaagaatgct ataatgcaat
    76681 atggttcagt gacatcaggt tatgcacatt attcaacata ttttaataaa gatgaaacag
    76741 catataactg tacaaataag agagctccat taaatcacgc tgtagcgata gtaggatggg
    76801 atgataatta ttcaaaagat aattttgcat ctgatgtaaa accagaatca aatggagcat
    76861 ggttagtaaa aagtagttgg ggagagttta attctatgaa aggattcttc tggatttctt
    76921 atgaagataa aactctacta acagatacag ataactatgc aatgaaatca gtatcaaaac
    76981 cagatagtga taaaaaaatg taccaacttg aatatgctgg tcttagcaag ataatgtcaa
    77041 ataaagtaac agcagcaaat gtatttgatt ttagcagaga ctctgaaaaa cttgattctg
    77101 ttatgtttga aacagactct gtaggagcaa aatatgaagt atattatgca ccagtggtaa
    77161 atggagttcc tcaaaacaat tcaatgacaa aacttgcaag tggaacagta tcatattctg
    77221 gatacataaa tgtacctact aattcttaca gcttaccaaa aggtaaagga gcaatagtag
    77281 tagttataga caacacagca aatcctaata gagaaaaatc aactttagca tatgaaacta
    77341 atatagatgc atattattta tatgaggcta aagcaaactt aggtgaaagc tacatacttc
    77401 aaaacaataa gtttgaggat ataaatacat atagtgaatt ttctccttgt aactttgtta
    77461 taaaagctat aacaaaaaca tcttctggac aagctacttc aggagaatct ttaactggag
    77521 cagatagata tgaaacagca gttaaagtta gtcaaaaagg atggacttct tcacaaaatg
    77581 cagtattagt aaatggagat gcaatagtgg atgctttaac agctacacca tttacagcag
    77641 caattgactc tccaattctt ttgacaggaa aagataactt agattcaaaa actaaggcag
    77701 agttacaaag attaggaact aaaaaagttt atctaatagg tggagaaaat tctttaagca
    77761 agaatgtaca aactcaactt agtaatatgg gtatatcagt agaaagaatt tcaggtagtg
    77821 atagatacaa gaccagtata tctctagctc aaaagttaaa tagtataaaa tctgtttcac
    77881 aagttgcagt ggcaaatggt gtaaatggac ttgcagatgc aataagtgtt ggtgcagcag
    77941 ctgctgataa caatatgcca ataatactta ctaatgaaaa aagtgagttg caaggtgctg
    78001 atgagttttt aaattcatct aaaataacta agtcttatat aattggtgga acagctactt
    78061 tatcatcaaa tttagaaagt aagctttcaa atccaacaag acttgcaggc agtaatagaa
    78121 atgaaactaa tgctaagata atagataaat tctaccctag ctcagatttg aaatatgctt
    78181 ttgttgttaa agatggttca aagagtcaag gagacttgat agatggacta gcagtaggag
    78241 cattgggtgc taaaactgat tcaccagtag ttctagttgg aaataagtta gatgaaagtc
    78301 aaaaaaatgt acttaagtct aagaaaatag aaactcctat tagagttggt ggaaatggaa
    78361 atgaaaatgc ttttaatgaa ctaaatactc ttttaggaaa atagtgaatg gaaagatagt
    78421 aaggatatac attttattat taaaaaatgt tcaatagaag tttataaata taaaataaca
    78481 aaaaggggtg tctcaaaatg aactttttag ttcatgaggc actctttttt gttgttttat
    78541 atttttatca tttaaataat gaaaaatagg attgtctata tttttagata gttttcttat
    78601 tattga
//
LOCUS       DAGSRM010000008        77690 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.008, whole genome shotgun sequence.
ACCESSION   DAGSRM010000008 DAGSRM010000000
VERSION     DAGSRM010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 77690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77690
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.008"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(23..1249)
                     /gene="murG"
                     /locus_tag="MJE33_000602"
     CDS             complement(23..1249)
                     /gene="murG"
                     /locus_tag="MJE33_000602"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBY9646571.1"
                     /translation="MKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIES
                     EIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDIVIGTGGY
                     VSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEDKL
                     VFTGNPVRKEILLSRKNIARKNLSISDEKRMVLCYGGSGGSRKINDAMRLVIKNMVNE
                     DIAFIFATGKSYYDEFMGSISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISLA
                     EITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLGD
                     RELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSASKKTKKEKVIKEVKEIKKET
                     TPSIEGQAKVIGIKKR"
     gene            complement(1267..2397)
                     /gene="spoVE"
                     /locus_tag="MJE33_000603"
     CDS             complement(1267..2397)
                     /gene="spoVE"
                     /locus_tag="MJE33_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HBY9646572.1"
                     /translation="MPKENLKKQIDIRMKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIEKNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGAALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            complement(2449..3804)
                     /locus_tag="MJE33_000604"
     CDS             complement(2449..3804)
                     /locus_tag="MJE33_000604"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBY9646573.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASIIVNDIKDENKLRN
                     ILDELKSINDIKYILGHHPEDVDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSNNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSSEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKANIFMNQTVEDFCV
                     LNYDDEIVKSLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIKDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            complement(3823..4791)
                     /locus_tag="MJE33_000605"
     CDS             complement(3823..4791)
                     /locus_tag="MJE33_000605"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBY9646574.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIITVVLAWISIIAVF"
     gene            complement(4832..6205)
                     /locus_tag="MJE33_000606"
     CDS             complement(4832..6205)
                     /locus_tag="MJE33_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBY9646575.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREANAQNAFVA
                     IVGENLDGHTFMKSAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGDISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLNKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     TNTLIVNGDDKFLSTLKEKEHVYKLKTFGFNKNNDVYCESYTMEEDSLTFICVINGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            complement(6372..8351)
                     /locus_tag="MJE33_000607"
     CDS             complement(6372..8351)
                     /locus_tag="MJE33_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HBY9646576.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILDE
                     DYKDIYALISKKNMALVKVKRWIDDDKASQIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGVEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIESFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDAKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            complement(8363..8710)
                     /locus_tag="MJE33_000608"
     CDS             complement(8363..8710)
                     /locus_tag="MJE33_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646577.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGAKCILLIATFSVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
     gene            complement(9191..10126)
                     /gene="rsmH"
                     /locus_tag="MJE33_000609"
     CDS             complement(9191..10126)
                     /gene="rsmH"
                     /locus_tag="MJE33_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBY9646578.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAISTAKERLSEYESRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKPIETTGELVDVIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNEGGRICIITFHSLEDRIVKNAFKHLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEIEVNPRSRSAKLRVAEKI"
     gene            complement(10156..10902)
                     /locus_tag="MJE33_000610"
     CDS             complement(10156..10902)
                     /locus_tag="MJE33_000610"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBY9646579.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKEAKRVGIKEDD
                     VLNIAIIAIPVGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVIGGIIAHVYLSKKNKNNISEE"
     gene            complement(11296..12582)
                     /locus_tag="MJE33_000611"
     CDS             complement(11296..12582)
                     /locus_tag="MJE33_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBY9646580.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLDVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPIGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYVNYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDRLSKSIERFYPEEPESVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINENFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKEIILDYIEKSKKEGLSKEEFERTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LDYLDVIKDITFEEVEERLKEHFKEEYCVISIVEPK"
     gene            complement(12597..13862)
                     /locus_tag="MJE33_000612"
     CDS             complement(12597..13862)
                     /locus_tag="MJE33_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBY9646581.1"
                     /translation="MVNTKKIINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTK
                     NALIPSIIASGSAKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYL
                     DESIFEEVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCI
                     EAMCKGERYSVSEFGYEDEIDSITSRELYEHYKNILKTSPIDIVVEGNFDEDKVVDII
                     SKNLKFEREEIINIPRADFIKNVDEVKVIDEQMEITQGKLVMGYRANVDYADIDKYYA
                     LVVGSNVLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSIMFISSGIETKDYDKAVEL
                     IKKQVESVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFVFSQSMAKTNSEVQDI
                     ISSIEKVTKKDIVEAIKNIELDTVYFLRN"
     gene            14178..15275
                     /gene="ychF"
                     /locus_tag="MJE33_000613"
     CDS             14178..15275
                     /gene="ychF"
                     /locus_tag="MJE33_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBY9646582.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQTFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
     gene            complement(15754..18033)
                     /locus_tag="MJE33_000614"
     CDS             complement(15754..18033)
                     /locus_tag="MJE33_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBY9646583.1"
                     /translation="MLSDAKNNRYGLSIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEESFEVDNILSILKRQAINSSFKRM
                     GVILPNGTAYTTDGYIEDFSKRDYFLKSMQGEVVITGKLKDVIEDDKNTNSNINVYSV
                     PIYKDNEVKGVIFATHSTKLYEKILSVPTFNGKGYSYITRSNGDIVLNPSSKNANSTK
                     ENLFSYIGRSDSISKSSINNMKSNIKNFKSGSLSYSMFGKGYYLSYAPIGINDWYLFS
                     EVPKTAISEKSYAIIKLTLSACVVLIIIFTALTIYILFIHKKSKEKLEEFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKKNLVLIYFDIDKFKLVNDRFGYEEGDRVLKEIAEIIKN
                     IFKEQSVFSRISNDNFAIIFEKKNNRESIIEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEEGETNISTCLDKAMIAKTTVKRKYLKVYEIYAENLKETLIEERDIEQEMHDAL
                     KNEQFKVYLQPKVDLSTTKIVGAEALVRWQHPKKGLISPGVFIPIFEKNGFITELDMF
                     VFTQVCKNFKRWENENFPTFPISINLSRVHLENPGFISELERITKEYEIEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGNAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRFIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSLNRKETN"
     gene            complement(18304..19758)
                     /locus_tag="MJE33_000615"
     CDS             complement(18304..19758)
                     /locus_tag="MJE33_000615"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HBY9646584.1"
                     /translation="MKLNDIIQGLDIINVKGELNIDINNVQYDSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVAIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLEDYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTKADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGAIGQ
                     KYSDLCIITSDNPRTEEPEAIIKDILEGIDKKKENYHVVVDREQAIAEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            19922..20791
                     /locus_tag="MJE33_000616"
     CDS             19922..20791
                     /locus_tag="MJE33_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY9646585.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKDIQFEYHNHDFNKIIIFINGNVT
                     YFVDGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDATSDLEK
                     CFKIATENKCNRLRLNIHDIESIKSFIEQINISESNTEFGNEILRNALFIQLMIFLNR
                     IFLKNENMESTEDVFYDETIKKVLNYINSNIDDKLSIDSISSEFFISKYYLMRKFKSH
                     TGTSIHNYIVQKRLILAKSLITNGHLMSEVCNKCGFNDYSSFVRAFKKNYGVSPKNYI
                     NNKNPLDITLLDE"
     gene            complement(21004..23076)
                     /locus_tag="MJE33_000617"
     CDS             complement(21004..23076)
                     /locus_tag="MJE33_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBY9646586.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLAHF
                     PALANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMVDNSMYANIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIVTSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFMEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAIMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDESETNAEVIEMSKNELASEILIALGNKDNLKSLDACITR
                     LRLVVNDINKVDKEKLKSLGAAGVMVIGNNVQAIFGPKSDSIKSDIKDIIEGKTKVES
                     TVTKNVDKNKVKKNMEVANMENALVEKIDSLTNGEILDIEEVPDSVFSSKLMGDGFAI
                     KSSDGLIYSPVDGTIGVIFPTKHAIIIKSQKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNGLSTISPVVFTNLEQNQKLSIKKGVTTAKEENRVSIL
                     RDDIEKIG"
     gene            complement(23379..24221)
                     /locus_tag="MJE33_000618"
     CDS             complement(23379..24221)
                     /locus_tag="MJE33_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBY9646587.1"
                     /translation="MIIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKSK
                     IERTFVIQDESFLDKISKLASQVDEKFFEVSTEIIAYAEENLNTKLYEYIYVALTDHI
                     AFAIKRYHENITIKNDLLHEIKRIHKKEYEIGKWAVDYINKEFDVKFPVDEAGFIAMH
                     IVNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRLLTHLKFFAKRLVK
                     KEKINDTNNEIIDIIKVKYEKDYDCAYKIKTHVEKNYDYYVSQDELLYLTLHIKRVIS
                     VLNL"
     gene            complement(24482..26524)
                     /locus_tag="MJE33_000619"
     CDS             complement(24482..26524)
                     /locus_tag="MJE33_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBY9646588.1"
                     /translation="MSILVTLLGLLVCTTISTVFSKKWSNIPLAIYQIVLGIILSILP
                     FKFSFSFNPEIFVICIIAPLLFSEGQNVSRKELLELRKPILLLAFGLVLITVFAGGIF
                     IHFLIPGMPLSVSFALAAVISPTDLVAVKSITQGLNFPKNMMSILEGESLLNDAAGVV
                     AFKVAVLATVTGVFSIEEAGIQFMITAFGGIIVGSILGYIIIKIRLSLHKWNLEEIPM
                     VIVIQIMTPLFVYFVAEEIGVSGILAVVMAGIAHGIEKEHLQNTTTKLRIISDNTWYV
                     LEYVLNGFVFTLLGFLLPSIYTGLSSKNENMALELTFISTLIVLILFVIRFIWVYLWH
                     NSFIKPKKNPLNNFFVGFLGFKDEEVVKESISKCKYSLIVATCGVHGTFTLATALSIP
                     FYLADKDVFPMRDTVLFISSEVILISLVLATVLLPRLLKNNVQESEPLLSDKEAYKLI
                     LKEAILKLSNEKAQEVQPVIHDLNEQLVDSEKGILNTPDNKLISMMIAEADQQGLLAV
                     LKLLEDGKISDRAFKLYRFYITKSRKSVQKSIFKIVKLKISMWIINRKVKKEVKEKIT
                     KIVSENKEIIKEFHHAYALSTKTAISFINKNTDEDNRHEALIAIRFYNRYLNWITRKI
                     QNDDKFEARVEYYRVMAIQSQRDSVQQLVETKQISREVAKELLENILYDEMMMIES"
     gene            complement(26676..27647)
                     /locus_tag="MJE33_000620"
     CDS             complement(26676..27647)
                     /locus_tag="MJE33_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HBY9646589.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFDKEIKCVKAVDGISFDLKKG
                     ETLAIVGESGCGKSTAGRTILRLIEKTEGKVEFNGVDIYKLEKEELRKLRPKMQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHNLVPKNEIKDNILNVVESCGLSKYHLNRKPGEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKGELYLHSQHPYTKALMSAVPIQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEIPVLKEISRNHFVACHLV"
     gene            complement(27648..28625)
                     /locus_tag="MJE33_000621"
     CDS             complement(27648..28625)
                     /locus_tag="MJE33_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9646590.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNEVSFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFRGKDILKMNEDELMGIRGNEISMIFQEPMTS
                     LNPAFTVGSQIIEGIMIHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVLVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPALGKNKDKRLYSIPGQV
                     PNPIGMPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
     gene            28844..30403
                     /locus_tag="MJE33_000622"
     CDS             28844..30403
                     /locus_tag="MJE33_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBY9646591.1"
                     /translation="MKFKKLASLILVSSLMLTFTACASNDKDKSSGSKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNAD
                     YFGGEPKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTE
                     EGVEGYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDAL
                     WEKGFTELNKEKREEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIKDAKPSPAI
                     LFEDWSKLYVE"
     gene            30509..31468
                     /locus_tag="MJE33_000623"
     CDS             30509..31468
                     /locus_tag="MJE33_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9646592.1"
                     /translation="MLKLILNRVKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     SQDIENIRVNMGLDKPVYIRYLNWLKNTLSGNLGISYINHMPVTEQIMEKMGNTFILM
                     GTSLIFSILVAIPLGIFLAVNKNSITSKVSSVFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIGDLVKHLVLPVITLGLYNTAIFTNYVEASVNEQLKKQYVITAR
                     AKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            31471..32283
                     /locus_tag="MJE33_000624"
     CDS             31471..32283
                     /locus_tag="MJE33_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9646593.1"
                     /translation="MFTSILKKLKKDKSFAFACITLSIIIILIITSFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLVDMLMSIPSFLVLIVLNTYLTPKVSTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQRHIFDKLYLAVFPGLLIFLTVMSLTIISKKFSSNT"
     gene            complement(32499..33401)
                     /locus_tag="MJE33_000625"
     CDS             complement(32499..33401)
                     /locus_tag="MJE33_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBY9646594.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEESE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKVRIGIPPL
                     ILGIAFSDVVAQLVANNPDIEFDIVEKGAYDLNRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNHPITWKVILCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
     gene            33756..34934
                     /locus_tag="MJE33_000626"
     CDS             33756..34934
                     /locus_tag="MJE33_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBY9646595.1"
                     /translation="MKDVVIVSAVRTPIGSFGGVFKNTSAVQLGTIAVKEAISRVRLN
                     LSEIDEVIIGNVLQTGLGQNVARQIAINAGIPNSVPSYTVNKLCGSGLKSVQLAAQSI
                     TSGENDVVIAGGTENMSQAPYIVPTARFGSKMGDITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQNKAENAIKNNRFKEEIVPVDILIRRGKIETIDKDE
                     YPKLGMTFEGLAKLKPAFKKDGTVTAGNASGINDGAAMLILMSQQKADELGIRPLAKI
                     KSYASAGVEPEVMGTGPIPATRKALKKAGLSINDIDLIEANEAFAAQALAVKNELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVETGLATLCIGGGQGISMVVSR
                     "
     gene            34943..35599
                     /locus_tag="MJE33_000627"
     CDS             34943..35599
                     /locus_tag="MJE33_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HBY9646596.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLVEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKITIVEADEIVEIGDIDPNEVNTPGIFVNYIVEGGNL
                     "
     gene            35599..36264
                     /locus_tag="MJE33_000628"
     CDS             35599..36264
                     /locus_tag="MJE33_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HBY9646597.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNV
                     ILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     VLGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKKINPAFTLDDVISATEAPLILSDSLNGKI
                     SVV"
     gene            36369..37145
                     /locus_tag="MJE33_000629"
     CDS             36369..37145
                     /locus_tag="MJE33_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HBY9646598.1"
                     /translation="MVKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQESLNNT
                     ITELQKKGYDCMSVKCDVTKEEEINHAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGITGQACLLDGGYTAQ"
     gene            37207..38541
                     /locus_tag="MJE33_000630"
     CDS             37207..38541
                     /locus_tag="MJE33_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBY9646599.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVILNKMD
                     VVTSMLGADKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKFGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSIVGSIGCATFGLVYMKYALNKSIRKG
                     ETYATYTSELEEESLDRELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSAFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
     gene            39038..39964
                     /locus_tag="MJE33_000631"
     CDS             39038..39964
                     /locus_tag="MJE33_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HBY9646600.1"
                     /translation="MMRRNIPTKLKTILALGVLTLATASSAVTASASSLENNVNISNK
                     SLVYSNTKYKFSAKPNCSVKPELKPDKDNSCKDKNHNDKNCNNTNKPEDNNNSDSTNK
                     PDNNKPESNKPEDNNNSGSTNKPDNNKPESNKPEDNNNSGSTDKPDNNKPESNKPEDN
                     NNSDSTNKPDDNNNSGSTSESFSAYQKEVVDLVNIERAKAGLNPLTLDSSISNVATKK
                     SQDMIDNNYFSHNSPTYGSPFDMLKKFGISYKTAGENIAMGQKTPKEVVNAWMNSEGH
                     RKNIMNPNFSKIGVGVAQKSGGSIYWTQIFVG"
     gene            complement(40357..43896)
                     /gene="nifJ"
                     /locus_tag="MJE33_000632"
     CDS             complement(40357..43896)
                     /gene="nifJ"
                     /locus_tag="MJE33_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBY9646601.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLIAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     GIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGITMSHLRFGDTPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHACIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRIQV
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKVDVMPANT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMDICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVLEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
     gene            complement(44414..44983)
                     /locus_tag="MJE33_000633"
     CDS             complement(44414..44983)
                     /locus_tag="MJE33_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBY9646602.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
     gene            complement(45055..45663)
                     /locus_tag="MJE33_000634"
     CDS             complement(45055..45663)
                     /locus_tag="MJE33_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBY9646603.1"
                     /translation="MDSNDKKGLKSPEIIIINGSPRINKNCSFIANEVKAMLEECGLS
                     CKIFNIYNMDIEYCTACGFCEKTGYCKFNDDMKPLYKMFDDSKGTIVISPVHFDSISA
                     KLKTVVDRTQAIYASKYVLNKPSIDRSKKRIGMYIAVGGSKSYDSQFKGGQIVMDFFF
                     KSINTKLLHNIYLNNTDDVSYIDNQEFKDIIYKNLKIYIDSI"
     gene            complement(45671..47434)
                     /locus_tag="MJE33_000635"
     CDS             complement(45671..47434)
                     /locus_tag="MJE33_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HBY9646604.1"
                     /translation="MTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPTKA
                     CVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKIIY
                     VENVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYITEQDIDFI
                     SSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSKQA
                     CNITNSKEALIIINSHHNKDAIVSCYNVEQKKKTNLYPMIDMFLKKESLGGYLNNLKP
                     CSKKGINLESYIYYKLQDKNDTIGCIVCANSKNYTSDDLNILSILSKQVSVGMQLYEH
                     NEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYATKV
                     DDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFDKM
                     GVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMEDATYE
                     NNTLSMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKLDL
                     KNFSSKDNYDDDILIVMLKDR"
     gene            complement(47477..47944)
                     /locus_tag="MJE33_000636"
     CDS             complement(47477..47944)
                     /locus_tag="MJE33_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646605.1"
                     /translation="MKMLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKI
                     DSVFFKLLKLAFGSILLMVAFNLNMVLGIGVFLVGVVYILYKRNMEEQVKEHINNVKN
                     NIDISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
     gene            48127..48588
                     /locus_tag="MJE33_000637"
     CDS             48127..48588
                     /locus_tag="MJE33_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646606.1"
                     /translation="MQIEQLDLETRNKIYCYTKKILRKYQKGITSGKLTADKFADNIL
                     SQDFISSILNEKIVNETNFKISYRNYIETLINIQNENLSNLRKKSTKTAKCFNISQIT
                     QLKNLLSNTGYNLLIPYKYLTARDIEGIVTLINTGSIELGNERIYNYISKA"
     gene            complement(48681..48851)
                     /locus_tag="MJE33_000638"
     CDS             complement(48681..48851)
                     /locus_tag="MJE33_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646607.1"
                     /translation="MSSVNFEDLKNKFINSDLDEKIRIYTTTEGLSVEQFKELLKYYP
                     IQHLSKLEKALG"
     gene            complement(49040..49261)
                     /locus_tag="MJE33_000639"
     CDS             complement(49040..49261)
                     /locus_tag="MJE33_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBY9646608.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
     gene            complement(49345..50571)
                     /locus_tag="MJE33_000640"
     CDS             complement(49345..50571)
                     /locus_tag="MJE33_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBY9646609.1"
                     /translation="MKKVVIIGGGPAGMIAASTACEKGYDVTLIEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNDDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNDKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIIDTKPSLIGIEVQESFTKDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTKFGLDGPIIKSASCRMKDTRKENYTILLDLKPALDEEKL
                     DKRVQKDFQKYTNKKFEKALDDLLPKKLIPIIINLSEINPNTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIEEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            complement(50633..51565)
                     /locus_tag="MJE33_000641"
     CDS             complement(50633..51565)
                     /locus_tag="MJE33_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBY9646610.1"
                     /translation="MDIQLNILLFFQNLRNPILNFIFLVFTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYLHLIGAVMVLGVGLSRLYLAVHWPIDVIAGWILGIFFTVVF
                     VKIFDYIDDNKNYILLVVLLIPFIIVAIFLNSPEYFEKFGIIVGFVFGYMVEDRFVKF
                     NTDNNNKKINFSNKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKLFISMLIVTLNISD
                     STMSIIFMNFIKNTVVVFYGIAGAPALFKLLKLN"
     gene            complement(51654..52181)
                     /gene="hpt"
                     /locus_tag="MJE33_000642"
     CDS             complement(51654..52181)
                     /gene="hpt"
                     /locus_tag="MJE33_000642"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBY9646611.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            52410..53486
                     /locus_tag="MJE33_000643"
     CDS             52410..53486
                     /locus_tag="MJE33_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBY9646612.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSIDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSIEKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVREILKNTDSFLMIDETYVEFTDTDTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNLDLWNINIIASKMGEIMFSDLDFISNTISLMNTERDY
                     LLKELKNIKSLDIYNTKGNFILCKIKTKELTAKSLREQLLPQKIIIRDCCSFEGLDEY
                     FFRVCILKPNENKLLISSLKAIFK"
     gene            complement(53572..54780)
                     /locus_tag="MJE33_000644"
     CDS             complement(53572..54780)
                     /locus_tag="MJE33_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HBY9646613.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFAVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGNKEEQSA"
     gene            complement(54941..55330)
                     /locus_tag="MJE33_000645"
     CDS             complement(54941..55330)
                     /locus_tag="MJE33_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HBY9646614.1"
                     /translation="MEFLKIIINIVLDILKKILVRFKNAKFGLVFVFDLLKLPDFMTD
                     KRINIVDKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDAIVLIWSIGIVNEEIN
                     KYRVIAKKDKHSNIIENVEFSIKDEEE"
     gene            complement(55434..56435)
                     /locus_tag="MJE33_000646"
     CDS             complement(55434..56435)
                     /locus_tag="MJE33_000646"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HBY9646615.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEGIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     misc_binding    complement(56494..56749)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(56883..58073)
                     /locus_tag="MJE33_000647"
     CDS             complement(56883..58073)
                     /locus_tag="MJE33_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBY9646616.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQKDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEINRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIVQKPVYYPFEAKIKSNNRDVVNNPLIYENGTYRMDYDDLEE
                     KAKCSNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLIRKGFKH
                     TPLQSLCPEYKDKIVTCIAPSKTFNLAGMQLSNIIVNNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNESEDWLNELNDYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMFKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMDRLKTI
                     FENL"
     gene            complement(58354..59475)
                     /locus_tag="MJE33_000648"
     CDS             complement(58354..59475)
                     /locus_tag="MJE33_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HBY9646617.1"
                     /translation="METNKDYIEYVHKVYDYLHTIPEKGFEEYKTSEFVLKELEKMGF
                     ENINKGIAGTGIIVTIDSKKEGPVFAIRADMDALEFIIDGEKKMIHACGHDGHTSMLL
                     TAAKLIKERNLVKRGKLKILFQPGEEKLYGALRVIDSGLLDDVEEMVGMHLRPGQDCR
                     LGKAIPAMYHCASCIMEFTIKGITCHGSKPHLGVNAVDAVCLAVNAINAIRVNPAIPN
                     SIKVTNISVKGETYNNIPDEAFMALDIRAESNDIIEELKEKAIHAVKTAVSSINAEAV
                     LISKDGVPAAEFDEELVNLAEETIKDVLGADASIGIFKNSGGEDFHYYTQKLKCKTTY
                     IGLGADATPGFHNPNVTFDTKALEYGVDIWCRLVEKRLG"
     gene            complement(59536..60021)
                     /locus_tag="MJE33_000649"
     CDS             complement(59536..60021)
                     /locus_tag="MJE33_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBY9646618.1"
                     /translation="MSEVAKKEVKVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVAATAIMTIFISMGGASGVIAGLFTAGQI
                     SQAHVAILLPMLLLSGAQIQFMGRLLGTAELKTKYYPHMFVIATLNGCIAMLIMNYFV
                     V"
     gene            complement(60023..60691)
                     /locus_tag="MJE33_000650"
     CDS             complement(60023..60691)
                     /locus_tag="MJE33_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646619.1"
                     /translation="MSKLESTTEKVEVKWYGYVALILGALFFSGIFKDAPGPLKVLDF
                     NNVLGYFGTLGTINDGVGTLAANFRGDGGTGPRDGWLYALTLIPSVMFALGVVRVIDH
                     LDGMKAAQKLLSPLLKPLLGLPGFAGLTLIASLQSTDAAASMTKELKDDGYIDEKQKA
                     VFCAFQFSGASAITNFFASGAALFPFIGDVPIFIPLALILIMKFVGANLLRLYLNKFD
                     KGEA"
     gene            61081..63105
                     /locus_tag="MJE33_000651"
     CDS             61081..63105
                     /locus_tag="MJE33_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY9646620.1"
                     /translation="MDKKKLILITHDKLNSDHYKEELTNFFGDEIIIETQNILDGIKE
                     NLEGDVVLSLSPLTSNFLIKHFKEDIEIIHGTKALSKLGYEKMMKLPPGTKSLLMTTN
                     KTSAFEMATYLYKIGINHIDFVPTYPDCDEIYDLDTAITPGQIRFIPKYIKNIVDLGW
                     RKISLDTYMSLLVVLKLKNEKFIEKLYKLSKETLSHDFLNTSLDNISKLKTILYMTID
                     EIGDGLIFFNTFNKVTFVNKSLLNMLGLDEKLIKSPSLMEYMPNSFLDKITKNLNIDN
                     MIIYIDEIDKKFILSKKPFYLYKNIEGCLITLKDVNNIEILEQKIRSDSVKRGYVAKY
                     KFNNIIGNSSIIKDCIKKAKKMALTDNPILITGETGTGKEAFTQSIHNHSNRKNKPFV
                     AINCASLPSELLESELFGYEDGSFTGAKKGGKKGLFELAHTGTIFLDEIGDMPHDLQV
                     KLLRVLQEKEIRKIGGTSIIPIDVRILAATNKDLEKLIIENKFRMDLFYRISMFTLDL
                     PPLRKRLEDIPLLLESFLKELPYKNIKLDKSLLEALNSYTWMGNIRELRNCIEYMAYM
                     GSNYLTINDLPQNISSKLNNNHMSSNMSIFNDLNQYDKNICISILKSLHMKPMGRTKL
                     MKFMEYNVTEYEVRNMLEYLTRNGYLISSKGRKGSSITEKGIKIIENNII"
     gene            complement(63335..64522)
                     /locus_tag="MJE33_000652"
     CDS             complement(63335..64522)
                     /locus_tag="MJE33_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HBY9646621.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKIIDDFKVAVVTQ
                     PLSENKVQYNMVEEMAKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDKEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDVNIAKRLEMIKETCKNIGLPFVQV
                     NTPNINTEEDKNKVKQFLNEDIEKQVKKYGKDINVFGVNEYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISEKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSVAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
     gene            complement(64724..65995)
                     /gene="brnQ"
                     /locus_tag="MJE33_000653"
     CDS             complement(64724..65995)
                     /gene="brnQ"
                     /locus_tag="MJE33_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBY9646622.1"
                     /translation="MRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLIGFVV
                     ADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCATTYEM
                     GIMPIFGDHGPVAKAVFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVLIVKG
                     IISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSDKDQI
                     GMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQVGKVI
                     LGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSIIAIS
                     VPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVPFIQS
                     LPLAGLGFNWVIPAILGGIVGNFIKSNKQVA"
     gene            complement(66136..66789)
                     /locus_tag="MJE33_000654"
     CDS             complement(66136..66789)
                     /locus_tag="MJE33_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBY9646623.1"
                     /translation="MTKIIKVQKPKFTGELEIIENLEDILEDIFNDEKIFNKIIENEV
                     ISDLDSVRVSFESCIFRNVSFENCNLKKIDLLDVVFENCNLSNTFFDGGSIYRVEFKN
                     SKLIGVRFDDCILKNVLFKDVLGKYSNFSFSKFKIVSILDSNFENSVFQEVKGDNLVF
                     EYTNLKKANFNGTKLSGIDFTTNNIEGIEIGIDDIRGAIFDVSQAIDLTKLMGIIIK"
     regulatory      67118..67219
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            67292..68635
                     /locus_tag="MJE33_000655"
     CDS             67292..68635
                     /locus_tag="MJE33_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBY9646624.1"
                     /translation="MVPNTNNVQPGGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     gene            68814..70178
                     /locus_tag="MJE33_000656"
     CDS             68814..70178
                     /locus_tag="MJE33_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBY9646625.1"
                     /translation="MSQLLIKNAYLITMNADREVYENGSILIENNIIKEVGKFDINIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYED
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLVRTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEIDLFRLHDVK
                     VSHDPGSAMKVVLGFASIPEMLEKGIPVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKKQASKIKDKAEVKIPSKF
                     KIIK"
     gene            complement(70356..72221)
                     /locus_tag="MJE33_000657"
     CDS             complement(70356..72221)
                     /locus_tag="MJE33_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HBY9646626.1"
                     /translation="MENVFKRLQEFNGYDGYKESFEMNYLCIYESIPLREQVELANNL
                     VDEILNMYKSESNEIYLLEGSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKEL
                     IYKSKKVEYVKLGLVLSEKYLNVENLREVVDTFSKSGEYVFYLSNTIKKLEFYNTYLF
                     NLSKKATGSIKVFAIVNMENLDSKINSYLIEDGYKDTKYERLLMNYIISIVDLNEYLE
                     KRDLDKEKINNLARLICNYLLSVEFKYIGNKLELVNRFLPTVVNYGTNFESLYSIFLI
                     AINVLKDENIECNKIEFEKEINGILLSEKWKNIYFEALRDASGKTEDIIKMSEIYDVN
                     LSFDDLLPYLNRDIRDFEVYWHISKKGTTSSRLKLLNFFEETFKIDDLIGKMKDIEKD
                     KLTQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSL
                     EELKIVKEAYEKEKNVILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSIAV
                     AGSRFRNREYLEKELEKSKIYYLTREKDNLYDEKAIKIVGETGYVIGYVPRKENYILS
                     NLLDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSRIKLIN"
     gene            complement(72457..73674)
                     /locus_tag="MJE33_000658"
     CDS             complement(72457..73674)
                     /locus_tag="MJE33_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY9646627.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYVFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVVDAFLIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLVVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYEKEKVYDYDV"
     gene            complement(73874..74737)
                     /gene="aroE"
                     /locus_tag="MJE33_000659"
     CDS             complement(73874..74737)
                     /gene="aroE"
                     /locus_tag="MJE33_000659"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBY9646628.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFKKLDLDYRYLAFDI
                     TVDKVKEALLAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENGVEVKGKKITIMGAGGAATAIQVQCALDGAREISIFNPKDDF
                     YKRAEQTVENIKKDVPECVVNLYDLEDTNKLYEEIQSSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPKKTKMIEDAEANGCKAIGGLGMLLYQGAEAFNLYTGLEM
                     PVEEVNELCFK"
     gene            complement(74819..76741)
                     /locus_tag="MJE33_000660"
     CDS             complement(74819..76741)
                     /locus_tag="MJE33_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBY9646629.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YLERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKVAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEVHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVPGGIDVHDSSVIAAYLEDCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPIACLANPILGHEAELKPAEISKEVLVVGGGVGGML
                     AAWVCAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDVVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHEVTVIELREEVGADVISEHRKFLMNDFENYNIHTITGAKVS
                     KFYDDGVDYLLTDGTEHDLKGFDSVVLAMGSRNYDPLSEVIKEVVKETYIVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            complement(76820..77668)
                     /locus_tag="MJE33_000661"
     CDS             complement(76820..77668)
                     /locus_tag="MJE33_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBY9646630.1"
                     /translation="MNNQKNLNIPITISSYTLGTEVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMIAILNEYDMKVTEVEYITQWGREEDRTEAQREKEQNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTAEDLAPVPADKIVSIQVCDVLETPYEKLRDESLHDRLA
                     PGEGYGNTEEFVKIIKEHNISPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
BASE COUNT        26148 a        13131 c         9177 g        29234 t
ORIGIN      
        1 taaattaggg gtaaatattt acttatcttt tctttattcc tattacctta gcttgacctt
       61 ctatacttgg agtcgtttct ttttttattt cttttacttc cttaataact ttttcttttt
      121 ttgttttttt agaagctgat ttttgtgttg aattgtatac tttcattatc tcatcatata
      181 taaggtctat ggcttcaggc ttacctatag ttttacttgc atttgccata tcaaccaaaa
      241 gttctctatc tcctaaaagt ttaaatactg ctgtatttag gctctctgga gttagatttt
      301 tttctaaaat agcaattcct gccccttgtt tttctatact cttggcatta tactcttgat
      361 gattttcagc agtataagct ttaggtatta ttatagatgg ctttccaaga gccgttatct
      421 cagctaatga aattgctcct gcgctaccta taaccaaatc acttgctgca agtgcatttg
      481 ccatatcttc taagtaaggc acaacttttt ggtatggttt aagatttata tcgctaatac
      541 ttcccataaa ttcatcataa tagcttttgc ctgtagcaaa tataaaagct atatcttcat
      601 ttaccatatt ctttattaca agtctcattg catcatttat ttttcttgaa cctccactac
      661 caccatagca aagtaccatt cttttttcgt cacttattga tagattcttt cttgcaatat
      721 tttttcttga taataaaatt tcttttctaa ctggatttcc tgtaaaaact aatttatctt
      781 ctgctgcctc tggaaatctt ttatgcgaat cttcaaaact tgtaagtacc tttgtaactg
      841 ttttagataa aatcttatta gttacaccag gaaaagaatt ttgctcatgt attatggctg
      901 gtattttccc catagatgca ttaaataaaa caggtccact aacatatcca ccagttccta
      961 taactatatc tggtttaaat ttcttaacaa tctttcttga ttgctccaaa cctttaaaaa
     1021 gcttaaaaac tctttttaca ttatcgaaat ctatttttcg cttaaaacct tgaactgtta
     1081 cagtctttaa ttcaaatcca tacttaggaa ctatttctga ctctattccc ttttccgtcc
     1141 caacaaaaat tatctcagcg tctggatgct catctcttat tttgtttgca atagctatag
     1201 ctggatacac gtgccctcca gttccaccgc ctgataataa aactttcatc aaatcatcat
     1261 ctcctgttaa ttaattttga catgctttga gatgtttaac actattccta cagctcccat
     1321 aaagatggtg agtgaagttc ctccataact tataaatggt agtgctacac ctgtagctgg
     1381 cattgatgat gtcgcaactg ctatatttaa agctgcttgt attccaattt gtgcacctat
     1441 acctataaca accatacaag caaatacatt agaagtcttc aaagctattc ttacacatct
     1501 gtagactaaa actacaaata gcataataac tattatacaa ccaatcaatc ctaactcttc
     1561 tcctattata gcaaatataa agtcattttg tggctctggt atataaaaat acttttgttt
     1621 actctttcct aagcctagac caaacaatcc acctgaacct agtgcatata gaccttgtat
     1681 tacctgatac cccttaccaa gagggtcttg aaaagggtct aaaaatgatg taacacgact
     1741 aagtctatag ggttcagcaa ttactaatgc agcaaataaa gcagccccac tccctatcat
     1801 agcaaataca attttcatat ccattccagc aacaaatatc attacaaaag taactagaat
     1861 tacagtacct gctgttgaca aatttggctg aagtattatc aacgcaaaaa atatccctgg
     1921 aactacgaga agtggtacaa caccttttgt caaagatttt attttatcat aatttttttc
     1981 aattaatttt gcagttaata ttattgtagc aaactttgcg atttctgcag gttgaaatgt
     2041 taaagctcct attcccaacc atctttttgc tccgtttgct tctatcccta aaggggtcaa
     2101 aactaacaat aataatacta ctgctatagc tccaattgct gtagcatttt tcttccaaaa
     2161 actataatct attcttgatg taataatcat aactataaat ccaagtacag catatattac
     2221 attctttttt agaaaatagt acgcatcatt gtgtttaaat gaagattgta taaaacttgc
     2281 actaaacacc ataactattc ctacaaatac taaaagcata gtagtgtaaa acactactcc
     2341 atcaaattca gtcttcatcc ttatgtcaat ttgttttttc aaattttctt taggcatcca
     2401 actaatctac ctccattctt ccatttaagt aagtttagag gtaagagatt attttaaatt
     2461 gtttacatta tctttgaagt cttttcctct tacttcaaaa ctttcataca tatcccaact
     2521 tgcacaagct ggtgaaagaa gcactatatc tccacttttt gaaatttcat aagaagtctt
     2581 aactgcttct tccatatctt ttactatatg tatgtcatta aatcctttat ttttagcaca
     2641 attttctata tttgatgcag tttctcctaa taagaccaat gatttaacat atgactttgc
     2701 agtttcaaga agttcatcaa aattacttcc cttatccatc ccccctgcta taagtattat
     2761 aggtctatca taagactgta tagcttttat agtggaatct ggattagttc cctttgaatc
     2821 atttacatat attacattgt ctaaatttct tacaaactct tgtctgtgtt ccactccttt
     2881 aaatgtcata agtacatcct ttataacttc taaatcaatt ttgcagacat atgctattgc
     2941 tatagctgcc atacaatttt ctaagttatg acctccaggt aaacttacat catctttatt
     3001 tagaaatttg attttatcat ctatatctat tattatgtca ttattttcta aatatactcc
     3061 tccatttact tttttagttc ttgaaaagta aattactttg gcattgcatt tatcagctaa
     3121 agattttact atctcatcat catagtttaa tacacagaaa tcctcaacag tttggttcat
     3181 aaatatattt gcctttgctt ctatgtattt ttccatagta tggtgtctat ttaaatggtc
     3241 ctctgtaata tttaatattg cgcttacttt aggtctaaat tcatctatac tctcaagctg
     3301 aaaactgctt agttcagtta ctaacacaga ctcttcactt gaagtttcta tagtatctat
     3361 tacaggattt cctatattac caactacata tgtatctcgt tttgctcttg aaaaaatttc
     3421 tcctaccaaa ctagttgtag ttgtctttcc atttgtacct gtaatcccta taaaaattgg
     3481 attattcgat agtttaaaag ctaattctac ctcaccaatt atatacttgc ctgaattttt
     3541 taatttcaat atgaatggta ggtctagtgg tacacctgga gacactacaa ccatatctat
     3601 atcatctaca tcttctgggt gatgtcctaa tatgtatttt atgtcattta tagatttcaa
     3661 ttcatcaagt atatttctca atttattttc atcttttata tcatttacga ttatacttgc
     3721 acccaattta tctaaatgtt taattgtaga tataccagtt tttgcaagtc ctactaataa
     3781 aacttttttt ccttttaagt ccatttttca ttccccctag ccctaaaata ctgctattat
     3841 acttatccaa gctaaaacaa ctgttataat ccaaaaaata aatacaactc ttgtttctgg
     3901 ccaaccacac tgttcaaagt ggtggtgtat tggagccatc ttaaagaatc tctttcttgt
     3961 tgccttaaaa tatccaactt gaatcaaaac tgatagagcc tccgcaaaat aaattcctcc
     4021 aattataggt attataagca ctgagttagt tagaactgaa aatgctacaa ctgcacctcc
     4081 taatgccatt gaacctgtgt cgcccataaa cactctagcc ggatatgaat taaatcctaa
     4141 gaaacctaaa catgctccaa cagttgctgc tgctaaaact gcgacttctg tatttccagc
     4201 aatagaactt gcaaatagca taaagaatac agaaactatt aatgttactc ctgatgctaa
     4261 tccatctaat ccatcagtca agtttacagc atttactatt gctacgatta taaacatcat
     4321 tattggtata tataatattc caacatttat cacaaaatct gtaaatggta tcataagttg
     4381 tgaagcactt gatgatgatg tatactgata aaaagcaaca taaaaagata atgcaacttg
     4441 aaggattatt ttttgatatg cttttagtcc aagtgatctt tttagcttta tttttataaa
     4501 atcatctaaa aagccaataa aaccaaaacc tgcaatacat ataagaccta ctgccatatc
     4561 atgactaact tttactcttg taagtccagt tattagaatt gcaacaatca ttataattcc
     4621 acccatagta ggtgttccgt ttttagctag atgtgtttgg ggtccatcat ctctaactgt
     4681 ctgaccaaat ttaaattttc taagcattgg tataaatatt ggtcctatta taattactat
     4741 taaaaacgca atcaatgcgg tgtacgtaag ctctgttatt cctaacatca taaaactcct
     4801 ctcccttgtc aattaactca ttagtttaag cttatttatt tagatattcg actatttttt
     4861 ctaatctcat gcctcttgag ccttttacta aaatagtgtc tcctgttttt actaattcat
     4921 ttaacttgtt aaacacatct tctctattct caaagtgata tatattggct gaattgaatc
     4981 ctaattgttt tgcttcttct ccaataaaca ctgaattttc acctatagtt attataatat
     5041 cagtattatt catagatgat tttccaacta atctatgacc atactctgaa atttcaccca
     5101 tttctagtat atctccaagt atcgctaccc ttctaccttt atatcttcca agtatcttta
     5161 aagcagcatc catagaatct ggactagcat tatatgaatc attaattatt gttaattttt
     5221 cattttttat tatgtctagt ctcatttttg tccctttaaa attttttaaa ccttttttta
     5281 tataatctaa agaaatattt agacacaaac caactaatat agcagacatt gcattatata
     5341 tattatgttc tcctattgtt ggtataaata tttcctcttt tttcccattt attacacata
     5401 taaatgttaa actatcctct tccatagtat aactttcaca ataaacatca ttgtttttat
     5461 taaagccaaa tgtttttaac ttatatacat gttctttttc ttttaatgta gataaaaact
     5521 tatcatctcc atttacaatt aaagtatttg tttcatcaaa gtttgaagta atctccatct
     5581 ttgctttcaa aataccctct tgtgaaccta gtttttctac atgtgataat cctatattcg
     5641 atatgactgc aattttagga tttactatat ccactaaata ttttatttca ttgaatccag
     5701 acatacccat ctctattaca gcacactcat gttctttatt tagattaaat aaagtcaatg
     5761 gaactccaaa atgattattt aagttttttt cattttttag tatatttaac tttgcagata
     5821 tggcagagta aatcatatct ctagtagtag tttttcccac actacctgta acacctataa
     5881 atggaatatc aaatttttct ttatagtatt ttgaaatatc tcctagagcc aaagatgtat
     5941 cttttacctc tattaaattt atatccgaac tttctaattt tattccatta ctttcatttt
     6001 ttataaaagt tttacatcca ctatcatatg cagacttcat aaatgtatgc ccatctaaat
     6061 tttctccaac tatggctaca aatgcatttt gggcatttgc ctccctgcta tctataacta
     6121 tatcacttac acagtcatta taatctccat atattaactt cccattagtt gcaagtacta
     6181 actcttttat tgttaagcac tccattatta aactcctcct ttaaaacctt agctatattc
     6241 tcttagcaac aaaaggcaat gtataccgtt atatatacat tgcctacgat aactcttaac
     6301 tttataatta tatactatat ttctgttttt ttgaagatgt ttatgggtat atgttaatta
     6361 tctgttactt tttagttttc aaaatatagg gttatacttg agtcttcatt aacttttact
     6421 ccaggttttg ggaatatatc ctttaccttt gtatcttctg gaagttctat atctgcatct
     6481 aagtctggtt ttaacttcgc atcctccaat gcctttacag catctcctat ttttagattt
     6541 cttacatctg gcactttaac ttgcttcttt tcatactcag ctttttcttc ttctttatat
     6601 actggtttaa ctcccaaata ttttaaagag tcattcatta tttctttcac tattggtcct
     6661 gctgtagtac taccaaatgc acttactcct gttggttcgt caacaatagc aagaactact
     6721 atttgcgggt catcacaagg tgctattcca acaaatgaac atatatattt tcctggagca
     6781 tacttaccat ctataacttt ttgagctgta cctgttttac caccaagtct ataacctggt
     6841 atatatgcta tctttcctcc accttcagtt acaactgatt ctgcaatttc taacattttt
     6901 ttagatgttt ctttagatat aacacttctt actttttttg gttttactgt ttcagttata
     6961 tttcctttat tgtcagtata agattttacc actcttggtt gcattaaatc tccaccatta
     7021 gctattgatg atatagctgt tataagttgt attggagtta ctgatataga ttgaccaaat
     7081 gatattgtag ccaattcaac tggtcctaca tttttttcgt tatacagtat tcccttagct
     7141 tctcctggta aatctatacc tgtcttatcc ataagaccaa atgattctat ataatcatac
     7201 atttttccaa cacctaatct gcttcctaac tcaacaaata ctgggttgca agagttttgt
     7261 actgcttgtt taaattcttg tgttccatga ggtctgtaat gtctccaaca tttaatcttt
     7321 cttcctccaa cagttacact acctgtacat gtgaattttt ctccatcttt aattactcct
     7381 tcttcaagag cactggaaga tgtaataagc ttaaatgtag aacctggttc atatgtatca
     7441 cttactgctg gatttctcca catttgataa tagcctttta ttttatcttt atcattatat
     7501 ttttcaagtt cttcttgata atatggatat attggcgttc ttgagtcatt agggtcataa
     7561 tctggttttg atgctaaagc aagtatatca cctgttttag ggttcatagc tattgcagta
     7621 accttttttg cattatttaa ctcataagct ttctgtactg ctttttcagt atagtgttga
     7681 attacttcat ctatagacaa cacaagacca ttaccttgta caggctcata atatttttcc
     7741 attccttgtg gtatttctct tcctgaagca tcagtactta ctataagttt tccaggcttt
     7801 ccttttaact ttttatcata ttgcatttct acaccagata tacctgttgc atctgaagaa
     7861 gtatgtccta acacatatgg tgcaaaattc ccatatgggt aatatctttg attatcttct
     7921 gccacccata ttccacttaa tttagcatct cttatttgac ttgccttatc atcatctatc
     7981 catcttttta cttttacaag agccatattt tttttgctta ttaaagcgta aatatcttta
     8041 taatcttcat ccaaaatctc agctaccttt tcggctgctt cttttttatc ttctacttcc
     8101 acaggtttac accaaacagt atacttagtt acacttactg ctaactcttt catatttcta
     8161 tcatatatag ttcctctttt gggttctatt ggtatatctc ttgtttgttg ttctagtgct
     8221 tttgtactca accaatttcc cttcataagt tgtagatacc cagtcctaat aacaaggcaa
     8281 aaaaacaatg cacatgctag aataagaacg agtaccagcc ttttcttact tatcctcttt
     8341 acttttctca aatctattcc ccctagttat ccacatctat atactttatt tgtccatctt
     8401 ttggatagtc catattgagc aattccttag cctttagctc aatttctttt attgatgttt
     8461 tttcagtttc ttttgccttt agttcctcta atactatctc tttttttctt aaatcttttt
     8521 gtaaataata gatatcgtac ttcatttccg agatttttga ataaccacat agattcaata
     8581 ttattactga aaaagttgca atcagtaaaa tgcatttagc tcctattttt ttcttatttc
     8641 tctttttata attattcttt tttttatttt tatattcatt tagattatgt attttataat
     8701 tttttttcaa attctcaccc cgatagtata tagtttaaat tttcctctat actatatttg
     8761 ttattaatca tatttcttcc tctaaaattt catcgctaaa ttaatacact attacacaat
     8821 ctaattgtat ttttttctat acacaacaaa aacctataaa ttcacttata cttttatatg
     8881 aatctatagg ttagatatta atttctattt atttttatca cttctagtat ttttagataa
     8941 tttcttccat aaatataata tgtaactagc tatctactct ctgattccta agttctccat
     9001 tatattactt aataccatct atatctatat ttttatatct aaattttcat tatatatctc
     9061 aataacaaag aacatttctt atagtgttaa ttgtttttta attgctttac cagtgcaaca
     9121 aaatatataa tactttttaa ttaattataa taatcttata tataagatta ttctttagcc
     9181 ttataactac ttatattttt tctgctactc ttagcttagc acttcttgaa cgtggattta
     9241 cttctatttc ttcctcacta ggtaatatag gttttctagt tattattttc acctcagatt
     9301 ctttatcaca ttggcatata ggtaaatgtt gtggacatat acaatctgat gctaaatgct
     9361 taaatgcatt tttcactatt ctatcttcta atgaatggaa tgttattata catattcttc
     9421 caccttcatt cattatagaa gaagcatcat ttatcatttt tgtaattact ccaagttcat
     9481 tatttacttc tattcgtatt gcctgaaaag ttcttttagc tgggtgaggt ccatctattc
     9541 tagctttctt agggatagct tttttaatta catctactaa ttctcctgta gtttctattg
     9601 gtttgtttgc tctttcttca actataaatt tagctatacg cttagcccag ttatcttctc
     9661 cataatcttt tattatctta gttaattcat cttctgtata agtatttaca acatcatatg
     9721 cagaaaactc acacctaaca tccattctca tatcaagtgg tgcatcttgc atataagaaa
     9781 atcctctatc agcttcatca agttgatgag atgaaactcc taaatcagct aatacaccat
     9841 caattttgta aactcctatt ttttctaatt cctcttttat attttcaaaa ttgctgtgaa
     9901 caaacttcac tctactttca tattcactca atctctcttt tgctgtgctt atagcatttt
     9961 tatcttggtc aaaccctata aataatccct tatctgacag tttttttact atttctttag
    10021 aatgtcctgc tcctcccata gtacagtcaa catacacgcc atctggcttt atatttaaat
    10081 tctctataca ctcatttaaa agaactgata catgatgaaa ttccatagtt acctccctta
    10141 aattcacttt ttaaactatt cttcactaat attattttta tttttctttg ataaataaac
    10201 atgtgcaatt atgccaccaa taactccaat tagacttata acttgtgcca ttctaagtgg
    10261 tcctaacatt aaactatctg ttcttaatcc ctctataaaa aatcttccta ttgaatataa
    10321 agttatataa gtaactataa cttgaccttc atatttttta ttttttctaa atagtaataa
    10381 aactataaat attccaaaat cccatataga ctcatataaa aaagttggat gtacctttac
    10441 tccatcaacc attattcccc aaggtaaatt ggtagcacct ccatgtgctt ctccattaat
    10501 aaaatttccc catcttccaa tagcttgtgc taaaggcatt ccaagtataa ctgtatcagc
    10561 catttttaag aagttaatat ttttaatctt agtgtatata taaccagcta atattccacc
    10621 aattaaccct ccatgtatag caagtccacc acctctaaaa ttaaaaattt gagacatatt
    10681 ttgagcatag taactccaat taaatacaac ataatagatt cttgcacaga ttagtccaac
    10741 tggaattgct attatagcta tatttaatac atcatcttct ttaattccca ctctttttgc
    10801 ttcttttatt gctattaaag ttcctaaaat cattccacat gccataagta ttccatacca
    10861 catgatgtct atgccaaaca gtgtaaatgc tactctatcc ataacgtcac ctctttgtat
    10921 taattgaaaa tatttccaaa gcaatatgct ataattacaa ttaattgtaa ttatactaaa
    10981 ttatgataac acattaatgt taattctaca aaaaatatta aaatttattc tacaactccc
    11041 tacactatat tatatctaat tactataaaa ttttttaaac tgaatctctc atgactaaag
    11101 ttacaagtgt acatagatat tttttaatca aaaagattga tgcatatata catcaacctc
    11161 ttctattcct tatctatagc tcttaaattt ttacctcaat ttatataaat atattttata
    11221 aatttgtttt accaaaataa acatttaaaa acataaacta atacttaatt agttattctt
    11281 attatgatta agtttctatt taggctctac aatagaaata acacaatact cttctttaaa
    11341 atgctctttt aatctttctt caacttcctc aaaagtaata tctttaataa catctaaata
    11401 atccaataga tttatatcct taaaaacata agatataaaa ctattaccta taaagtttat
    11461 agaatcaaag cacttaataa atgaccctat ttttttcttt ttagttctct caaactcttc
    11521 ttttgataat ccttcttttt ttgatttttc tatataatca agaattattt cttttacttt
    11581 tttaggctct ttagaatctc cagctattat gctaaaagca taatccactt gagacgaaaa
    11641 tccagctcca aaattttcat ttattaaacc ttgcatatac aaatcttcat aaagcttact
    11701 tcctttttta aataacatcc ctacaagtat atcagttaca atctctttac gtaaaagttc
    11761 tttccctttt aaaccaacat tgctatcttt aaaacctatg ttgaacatag gcatagatat
    11821 agggaatttt tctataacct ctttttcttt tacactttct ggttcttctg gataaaatct
    11881 ttctatactc ttagagagtc tgtctacttt gtaattgttt gactttttag ttacatctat
    11941 aactttttct acatctaagt caccaacaac aaataaagcc atatttcctg gattatagaa
    12001 agtattataa catttatata gctcttcctt agttatctta tatatactat caacagtacc
    12061 tgctatatca attctagcag gataatttac atacattgcc ttcaagcaat taaagtatac
    12121 attccaatca gggtcatcat tgtacatttt tatctcttgt gctattatac ctttttcctt
    12181 ttcaacattt tcatctgtaa aataaggtgt ttgaacatag tcaattagat gttctaaact
    12241 ctcataaaaa ttttcagtag cagaaaataa gtaggctgtc attgtaaaat tagtaaatgc
    12301 atttgcattt actcctaatt ttgaaaattt atcaaatgca tctcctccat caggttgctc
    12361 aaacatctta tgctctagaa aatgtgcaat accttcattc actctaattt tcttatcttc
    12421 tccaattggt acaaattcta agtcatttga accataatta gtagccaaaa tagcatactt
    12481 tttcataaaa ccccttttag gcataaaata tacatctaat ccattttgca atttctcata
    12541 ataaacttct tctttcaata tatcattaac tattttttcc atagacatct cctccattaa
    12601 tttctcaaga agtatacagt atcaagctct atatttttta tagcttcaac tatatctttt
    12661 tttgtaacct tttctatact agatattatg tcttgtactt cagaatttgt ctttgccata
    12721 gactgagaga aaacaaaatc agacatacca cctatattat cagttataga tttcatagaa
    12781 ttaactaaag caagtttact gttttctaat tcttcatcag atatatcacc tgcttttaca
    12841 ctttctactt gtttttttat caattctaca gctttatcat aatctttagt ttctatacca
    12901 gatgatataa acatgatact tttatacttc tctattgaag agaatatata gtaacataaa
    12961 ctttcttttt ctcttacatt aacaaataat ttagagtgtg gaccaccacc taagacattg
    13021 ctcccaacta ctaaagcata atatttatct atgtctgcat aatccacatt tgctctataa
    13081 cccataacta acttaccttg agttatttcc atttgttcat ctataacttt tacctcatcc
    13141 acatttttta taaaatctgc tcttggtata tttataattt cttctctttc aaattttaga
    13201 tttttactta ttatatctac aactttatct tcatcaaaat taccttctac aactatatct
    13261 ataggtgatg ttttaagtat gtttttataa tgttcatata actctcttga agttatacta
    13321 tctatttcat cttcataacc aaattcactt acactatatc tctctccttt acacatagcc
    13381 tcaatacatt tatcttgcgc atattccttt ttatcattta ttattgattg tatcttttct
    13441 cttaaattct ctttttcaat atttaaatat tcttctttaa acccaccatc tacaacaagc
    13501 gtattattta ttacttcatt aaaaaattct actacttctt caaatatact ctcatctaaa
    13561 tacttttcac ttatgttaat tactttaaaa ctcaatactt gacgttctcc acgttttaca
    13621 gcatcagctc ccatagatga accatataaa tcatctaatt tattggatat tgctctagca
    13681 ctaggatatt tagcgcttcc acttgctatt attgatggta ttagagcatt ttttgtagtt
    13741 tctttttcat ccaatagtct ctgaacatat aaacttataa gattcgattt aaacttttct
    13801 gtttttatca aagtaagatt aatattatta cctaaattta ttattttttt agtattaacc
    13861 atatgtacct cctattttta tttaaacatc aattttaaaa ttgaaattta tttatttttt
    13921 aattacatat actcacatat aaaaaccttc tatttaatta tatccttttt attattttct
    13981 aaaagcaata tttttattta aattaaatac aaatattatt taaatcaata tgctttaaag
    14041 tctagtttac aagcttgata tatatgatgt ataatcaaca tgtatgtcta taattaaaac
    14101 aattttgcaa tcctatagat tgattgttta aagttatata aagaatataa aaaagaataa
    14161 gagagaggac gattaatatg aaattaggta tagttggttt accaaatgta ggtaaaagca
    14221 cactatttaa cgcaataact caagcaggtg cagaatctgc aaattaccct ttttgtacaa
    14281 tagacccaaa tgtgggagtt gtatctgttc cagatgaaag attaaacaaa ctacaagaat
    14341 tatataattc tgaaaaaata gttcctactg ctatagaatt ttgtgatata gcaggtttag
    14401 ttagaggagc ttctaaagga gaaggtttag gaaataaatt cttatcacat ataagagaag
    14461 ttgatgcaat agttcatgtt gttagatgtt ttgaggatga aaatgttgta catgtagatg
    14521 gttctgtaga cccacttcgt gatatagaaa ctataaactt agaattaatt ttctcagata
    14581 tagagattct tgaaagaaga attaataaaa ctcaaaaagc tgctaaggct gataaaactt
    14641 taggttctga acttgattta ttaaaatcaa taatgtctac tttagaagaa agtaagtgtg
    14701 taagaactat ggaatttaca gaagatgaac aaacattcgt aaattcattg gatttactta
    14761 catctaagcc tgtaatatat gcttcaaatg tatcagaaga tgatttagct gataatggag
    14821 aaaataataa atatgttcaa caagttaaag catttgcaga aacagaagat gccgaagttg
    14881 ttgtagtatg tgcacaaata gaagcagaaa tttctgaact tgattctgct gaagaaaaga
    14941 aagagttttt agaaacatta ggtcttgaac aatcagggct tgataagctt ataaaatctt
    15001 cttatgcttt acttggttta atttcattct taactgctgg tccaaaagaa gttagagcat
    15061 ggactataaa agttggaagt aaagctcctc aggcaggagg aaaaatacat tctgatatag
    15121 aaagaggatt tatcagagca gaaacaatag cctttaatga tttggttgag catggtacta
    15181 tggctgctgc taaagaaaaa gggttggtta aattagaagg taaagaatac atagttaaag
    15241 atggagatgt aatattattt agatttaatg tttaaaatga ttaatcgtat acactttgac
    15301 tattatatat aataactaaa aaactaagat tataataata caatattacc ttataataat
    15361 tatatagaat ttaaaaataa aagaagagta tttcaaatga tttgtataaa ttattatgaa
    15421 aaactctttt tttgatataa taaaattact tacatatata acatttaaat ttataaatta
    15481 tctttttatt ttttaaaata tgtataacac acctatagaa attactatta ttactatcta
    15541 aagtattttg ctataatccc atatactctt acctcaaaaa catccaaatg taaaaaatac
    15601 tacatattaa taaactttgc ccaataattc ctaaaataat atcctaacaa tgatttatgt
    15661 ttttataata gtatattttt acataaaaaa gtgaacattg attaacaaag ttcacttttt
    15721 tataaatttt tatgttataa ttcacaattt tatttaattt gtttcttttc tatttaaact
    15781 atatctatct aatattaatt tttcatattc ttctattggc attggtctag caaataaaaa
    15841 tccttgggcc atatcacaac ctataaacct taaaaactct gcttgctcta cagtttcaac
    15901 accttctgaa acaactttta tatcaagttg ctttgccatt tgaacaatac ttgaaactac
    15961 aatttgactc ttttttgcat ttcccactgt tataaagaat tgcctatcta atttcaatac
    16021 atcaattggc atatctttaa gcatatttaa agaagaataa ccagaaccaa aatcatccat
    16081 agaaatttta aatccaacag atttcaaagt ttgcattatt ttaaataata tttttgtatt
    16141 gtcaaatatt gcactttctg tcaattcaat ctcaattaaa tttggttcaa tttcatattc
    16201 ttttgttatt ctttcaagct cagaaataaa gcctggattc tcaagatgta ctcttgataa
    16261 gtttattgat ataggaaatg tggggaaatt ttcattttcc caacgtttaa aatttttaca
    16321 aacttgggta aatacaaaca tatccaattc tgttataaaa ccatttttct caaaaatagg
    16381 aataaataca cctggactta ttagaccttt tttaggatgt tgccaacgta caagtgcttc
    16441 agcacctaca attttagtag ttgataaatc aaccttaggc tgtaaataaa ctttaaattg
    16501 ttcatttttt aaagcatcat gcatttcttg ttcaatatca cgttcttcta tgagagtttc
    16561 ttttaaattt tcagcataaa tctcataaac ttttaaatat tttcttttta ctgtagtttt
    16621 tgcaatcata gctttgtcaa gacaggtaga aatatttgtt tctccctctt caacaaaata
    16681 tacacctatg gatggaatta aattgagttc tactcccaaa cttgttttta tcattgacaa
    16741 ttttttacgt ataagttcac atatttctat aattgactct ctattatttt tcttttcaaa
    16801 tattatagca aaattgtcat ttgaaattct tgaaaataca gattgctctt taaatatatt
    16861 cttaattatc tctgctattt cctttaatac tctatctcct tcttcatacc caaacctatc
    16921 atttactagc ttaaacttat ctatatcaaa ataaattaaa actagattct ttttctcatg
    16981 agaggataaa aattttcccc cttcaagatt aaatttattt gaattgccaa tacctgtaac
    17041 gttatcttta aaagcaaatt cttccaattt ttccttactc tttttatgta taaatagtat
    17101 ataaattgtt aatgccgtaa agattataat caagactaca catgctgata atgtaagttt
    17161 tataatagca tatgatttct cagatatagc agttttagga acttcagaaa aaagatacca
    17221 atcattgatt cctattgggg cataacttaa ataatatcct tttccaaaca tactataact
    17281 taatgaacct gatttaaagt tttttatatt tgacttcata ttgttgatac tagattttga
    17341 tattgaatca cttctgccaa tgtaagaaaa taaattttct ttagtagagt tggcgttttt
    17401 actgcttgga tttaaaacaa tatcaccatt acttctagtt atataagaat atccttttcc
    17461 attaaatgta ggtacagaaa gtattttttc atacaacttc gtactatgtg tggcaaatat
    17521 aactccttta acctcattat ctttataaat tggtacagaa tatacattaa tattactatt
    17581 ggtattttta tcatcttcta ttacatcttt taatttgcct gttataacca cttctccttg
    17641 cattgatttt aagaaataat ctcttttaga aaagtcttct atataaccat ctgtagtgta
    17701 tgctgtacca tttggtaaaa taactcccat tcttttaaat gaactattaa tagcttgcct
    17761 ttttagtata gaaaggatat tatctacttc aaaagactct tctccttcaa taaatattga
    17821 aattgactct agtaacttta tatctccatt aatttgtttt tttaacacat tgacgctttg
    17881 atttgctatt tctgaaagat attgattcgt ttcttcacct aatgtcttat tcaaccttga
    17941 accataaatc aaacaggaaa ttgtaattac taagatactt atgacagcaa ttatggacaa
    18001 accataccta ttattttttg catcacttaa cattatgctt cctgcctttc tcgaatctat
    18061 gtatgtataa tcattatctt atgacaatta ttgtaaacat tttataataa tacttatttt
    18121 tccatattta ctgtaattat acaaaattat aaactttatt acaacaataa attaattaat
    18181 atagatatta gtgtttatat tattgcatta tctattttac aattatttac atataaaaaa
    18241 atgtaatatc aaagtgaagc ttgatattac atttttgttc ttgtattttg tttaattttt
    18301 atgctacatc ttacttaaac actcatttgc aacttcttta tcatcaaaat gatgcttaac
    18361 tttacctata atttgataag tttcatgtcc cttacctgtt ataattacaa catcatcctt
    18421 ttttgccata cttatagctt ctgctatagc ttgttctcta tcaacaacaa catggtaatt
    18481 ttcttttttc ttgtcaattc cttcaagtat atcttttatt attgcctctg gttcttcagt
    18541 tcttggatta tcagaagtta taatacataa atcagaatat ttttgtccaa ttgctcccat
    18601 caaaggtctc ttttcagtat ctctatctcc accacaccca aacacagaaa ttatttttcc
    18661 ttctgcaaat tgttgagttg tctttagtac attttcaagt gcgtcaggag tatgagcata
    18721 gtctacaatt acgcttatac ctttatcatt tggaactgtt tcaaaccttc ctgatactcc
    18781 attagaaagt ctaaggcctt ctttatatat tggtttaggt atgccaagta catagcaagc
    18841 agctataact gctaaagtat tatatactgt aaacattcct ggaactggta taaatattac
    18901 ttcttcatat gaaggtgtta ttaatctata agatactcca tccgaatctg attttatatc
    18961 tctagccata aaatcagcct tagtatcaac accataagtg taacaaggta catttatacc
    19021 ttttatattt tcatatatct tctttccacc ttcatcatct atgtttatta tatttgccat
    19081 agtagtctta aaaaataatt tttcttttgc ttttctataa tcctctaaat ctttatgaaa
    19141 atctaaatgg tctggtgtta aatttgtaaa tatgcctagt ttaaagtctg tttcatcaac
    19201 tctatttaat gccaatgaat gtgatgaaac ttccattgca cagtagtcac atccattatc
    19261 caacatatta ttaaaatgat attgtaaatc tatactttct ggagttgttg aattggaaac
    19321 aatgtctttt tctccatcaa aaatttttat agttcctata agtccaactt tattcttatt
    19381 taaagttaat atctcattta gtatagtagt tatactagtt tttccattag tacctgtaac
    19441 tcctataacg ttaaattttt gagatggatg attgtaaaaa ttatcagcta cttttgccat
    19501 atcttttctt gtatctttta cctttataaa tgtacatcct tttattgcta catcttcttc
    19561 aacaagaaat gcacttgctc ccttttctat ggcatctttt atatacttat gaccatctga
    19621 cacaaatcct tttatacata taaataatgt accttttgta acctttcttg aatcatattg
    19681 tacattatta atatctatat ttaattcacc cttgacattg attatatcca acccctgtat
    19741 aatatcattt aatttcataa atgctttcct ccctttaaac tctttctgct aaaattacac
    19801 tacatataat attatattaa gaatttttta ctatgtgaat ggttaaaaat gcttgaaaca
    19861 ttgcaaaaaa tgctctataa ttttctataa tataactgag gaaaggtctt ctataaaaac
    19921 tatggataat aaaatcgggt acttaaacga taactttaaa atatttaata ttagagataa
    19981 aaaagatata caatttgaat accataatca tgattttaat aagataatta tatttataaa
    20041 tggaaatgta acttattttg ttgatggtat tccctataaa ctaaagcctt gggatatact
    20101 ctttatcagc aataatgaaa tacataaacc agttatagat tcaaatgtct tttatgagag
    20161 aattgcaata tggattagcc cagaatttat gaaaaaaaat agtgatgcta ctagtgactt
    20221 agaaaaatgt tttaaaatag ctacagagaa taaatgcaat agattgcgtt taaatataca
    20281 tgatattgaa tctattaaga gttttattga gcaaataaat atttccgaat ccaatactga
    20341 atttggaaat gaaatattga gaaatgcatt atttatacaa cttatgatat tcttaaacag
    20401 aattttccta aaaaatgaaa atatggaatc aacagaagac gttttttatg atgaaactat
    20461 taagaaagta ttaaactata taaattcaaa tatagatgat aagctttcaa tagatagcat
    20521 atcgtctgaa ttttttataa gtaagtatta tcttatgaga aaatttaaat ctcatactgg
    20581 aacgtctata cacaattata tagttcaaaa aagattaatt ttggcaaagt cactaataac
    20641 caatggtcac cttatgagtg aagtttgtaa taaatgtgga ttcaacgatt attctagttt
    20701 tgtaagggca tttaaaaaaa actacggtgt atctcctaaa aattatataa acaataaaaa
    20761 tcctttagat attactcttt tagatgaata gataatttat gtaaatttta ttttaataaa
    20821 acttgaagta ttagtgtatt tatcttacta atacctcaag ttttttaatg ttttaaaata
    20881 tatcaaaata taatgatata tatttctatt acttaaaata tatattaaat aaattgcatg
    20941 ttaactgaac ctatttatat atttattaaa tctcacataa cttttagtta cactatcata
    21001 tagctatcct attttttcaa tatcatctct taatatactt actctattct cctcttttgc
    21061 tgtggttact ccttttttaa tgcttaattt ctgattttgt tctaaattcg taaatactac
    21121 aggacttatt gttgatagac catttttttc aatatattct aagtccattt taactaattt
    21181 ttctcccgct tttacttcat ctcctacatt cacaaatact tcaaatccat ttccttctaa
    21241 gtttacagtt tctatcccta aatgtataag tatttctact ccactttttt gggattttat
    21301 tattattgca tgcttagttg gaaatataac tccaatagtt ccatcaactg gtgaataaat
    21361 caatccatca ctagatttaa ttgcaaatcc atcccccatc agtttacttg aaaatacaga
    21421 atctggcact tcttctatat ctaatatctc tccatttgtt aatgaatcaa tcttttctac
    21481 taaagcattt tccatatttg caacttccat attttttttc actttatttt tatcaacatt
    21541 tttagtaaca gtactttcaa cctttgtttt tccctctatt atatctttta tatcagactt
    21601 tattgaatcc gattttggtc caaatatagc ttgaacattg ttgccaataa ccataacacc
    21661 tgctgctcca agtgatttca atttttcttt atctacttta tttatatcat ttacaaccaa
    21721 tctaagtctt gttatacacg catctaggct ctttaaattg tctttatttc ctaaagcaat
    21781 caaaatctct gatgctaatt cattcttaga catttcaatg acttcagcgt tagtttctga
    21841 ctcatcttct cttcctggtg tctttaaatt aaactttctt atcataaatc taaatacact
    21901 atagtataat actgcaaaac caatacctac caagatagcc aagtaccatg gagttctatt
    21961 aggaagaaca ccaaataata agaaatctat taatcctcct gaaaatgtga atccaatttt
    22021 aactcctaat aattgcataa ttgcgtatga taaacctgaa aataaacaat gtattgcaaa
    22081 taatactggt gcaacaaata aaaacgcaaa ctctattggc tcggttattc ctgttaaaaa
    22141 agaggttaat gctgctgatg ccattatacc tgctacaaat tttttctttt ctggctttgc
    22201 ttcatgcacc atagcaagag ctgctgctgg aagaccaaac atcataaatg gaaatgcacc
    22261 tgtcataaaa gtacctgctg tgaaatttac tccatcttta agttgagcaa agaatatctt
    22321 ttggtcaccc attacaagct caccagcttt atttacatat tctccaaatt gataccaaaa
    22381 tggattatgg aaaacatgat gcaaaccaaa tggtattaat agtctttcca aagaaccttc
    22441 cataaatgct gctagtgttt gatttgcttc tataacattt tttgagaaac taagtaatcc
    22501 ctcttgtatt ggaggccaaa taaaagacaa tataattcct attgctatag caactaatga
    22561 agtaacgatt ggtacaaatc ttttacctgc aaaaaaacct agatactgag gtaattcaat
    22621 tttataaaac tttttataaa gataagcagc cactatccct ataataatac caccaaatac
    22681 ccctgtttga attgttggta ttccaacaat atttgcatac atagaattgt ctaccatatc
    22741 ttctgtaaca cctgccataa cacccataga tgtattgaga attaaaaaac ctactatggc
    22801 tgccaatgct gcaacacctt ctccttcagt taaaccaaca gctaccccaa ctgcaaatat
    22861 aagtggtaag ttattaaata taatttgacc tgaactttcc attatactta ttataagttg
    22921 aaatgtagga ttcgccaaag ctggaaaatg agccaacaat gcgtctcctg ctaaagcatt
    22981 actcacgcca agtaatatac ctgctgcagg aagtaatgca actggtagca ttagagattt
    23041 tcctactttt tgtaaaacac caaatacttt tttcatttac ttttctctcc ttattatttt
    23101 atattttttt aaataaaaaa gacttaagtt tataaaaagt tacattagta tacttagttc
    23161 taaaattaaa cttatgttaa tataaataaa cctatattaa cctaatttca gaattaaaaa
    23221 taaactatta taattttttt ataaacataa gtcttgcctg cattatcagt aacaatctta
    23281 atgttttaaa attattattt tatattaaat tttaaataat atattaaaat taaacataat
    23341 atattaaatc tatacattct gtattaaact actctttatt ataaattcag tacagatata
    23401 actcttttga tatgtaatgt taaataaagt agttcatctt gacttacata gtaatcataa
    23461 ttcttttcta catgagtttt tattttatat gcacaatcat aatccttttc atactttact
    23521 ttaattatat ctataatttc attatttgta tcattgattt tttctttttt tactaatcgt
    23581 tttgcaaaga attttaaatg agtcagaagc ctatcataat ttatatcatc ttctttaaat
    23641 tcaactctat aataatatct tataatattt agtatgtctt ttacaatttt agtaattaat
    23701 agtgattcct ttgaacttcc cttatagttt gaatttacta tatgcattgc tataaaacca
    23761 gcttcatcta ctggaaattt cacatcaaat tctttattta tataatctac agcccatttc
    23821 ccaatctcat attctttttt atgtattctt tttatctcat gtagtaaatc attctttata
    23881 gttatgtttt catgatatct tttaattgca aatgcaatat ggtcagtaag tgctacataa
    23941 atatattcat aaagcttagt attcaaattc tcctctgcat aagcaattat ttcagtacta
    24001 acctcaaaaa atttctcatc aacctggctt gctagtttac taattttatc taaaaagctt
    24061 tcatcttgta ttacaaaagt tctttcaatt ttacttttat ctatttcttg acctttcttt
    24121 ttttgaaacc caattccaca tcctgttaat attaattctt tttttatatt ttcatctaaa
    24181 actaatacaa cattattatt ataaatttgt tgaataatca taatcaatat ctctcccaaa
    24241 atacaatttc ccttattcat aaaaaaatac ctaaattaag atacaattca cctgtatacc
    24301 ttaacctagg tattgcctgc ttaccagtaa caatccagct tgttaacgct tatatattgt
    24361 attcaattta tggttttatt ctattataaa taaatatata tgtcaagtaa attattgaaa
    24421 actatttcct attttttagt attaatttta acatcaaaaa tcaaatttta ttccattata
    24481 tttatgattc tatcatcatc atttcatcat agagaatatt ttccaataac tctttagcca
    24541 cttctcttga tatttgctta gtttcaacta attgttgaac actgtctctt tgactttgta
    24601 tcgccattac tctgtagtat tctactcttg cctcaaactt atcatcattt tgaatctttc
    24661 tagtaatcca attcaaataa cgattataga atctgattgc tattaaggcc tcatgtctgt
    24721 tatcttcatc agtattttta tttataaatg atatagcagt tttagtagaa agtgcatatg
    24781 catggtgaaa ctctttgatt atttctttat tctcagatac tatttttgta attttttctt
    24841 ttacttcttt ctttactttt ctatttataa tccacatgct tatttttaat tttacaattt
    24901 taaatataga tttttgaaca gattttcttg attttgtaat ataaaatcta tacaatttaa
    24961 aggctctatc agaaatttta ccatcttcta atagtttcaa aacagctaat aatccctgtt
    25021 ggtctgcttc tgcaatcatc atacttataa gtttattatc tggagtattt agtattcctt
    25081 tttctgagtc taccaactgt tcatttaaat catgtataac tggttgtact tcttgtgctt
    25141 tttcattact aagttttaaa atggcttctt tcaaaattag tttatatgcc tctttatcag
    25201 ataataaagg ttctgactct tgtacgttat tttttaataa tcttggcagt aaaactgttg
    25261 ccaatactaa acttataaga ataacttctg atgatataaa taaaacagta tctctcattg
    25321 gaaatacatc tttatcagcc aaataaaaag gtatagataa ggcagttgct agtgtaaatg
    25381 taccatgaac accacaagta gcaactatta gtgagtattt acatttagaa atactctctt
    25441 ttacaacttc ttcatcctta aaacctaaaa aacctacaaa aaaattattt aaaggattct
    25501 ttttaggttt tataaaagaa ttatgccata aatataccca tataaatctt ataacaaata
    25561 aaataagtac aattaaagta gaaataaaag tcaattccaa tgccatattt tcatttttac
    25621 tactaagtcc tgtatatata cttggtaaca aaaaaccaag cagtgtaaag acaaatccat
    25681 tcaatacata ctccaaaaca taccaagtat tgtctgatat tatccttaat ttagtagttg
    25741 tattttgtaa atgctctttt tcaattccat gagctatccc tgccattaca acagctagga
    25801 ttccagatac tcctatttct tctgctacaa aatatacaaa aagtggagtc attatttgta
    25861 taactattac cattggaatt tcttccaaat tccacttgtg taaactaagt cttattttta
    25921 taattatgta tcctaaaata cttcctacta ttattcctcc aaaagctgta atcataaatt
    25981 gaatccctgc ctcttctatt gaaaaaactc ctgtaactgt ggcaagtact gcaaccttaa
    26041 atgctactac tccagcagca tcatttaata aagattcacc ttcaagtata ctcatcatat
    26101 ttttaggaaa atttaatcct tgtgtaattg attttactgc tactaaatca gttggagata
    26161 ttactgctgc caaagcaaat gaaacagata atggcatccc aggaataaga aaatgtataa
    26221 agattccacc tgcaaacacg gttattaaaa ctaaaccaaa tgcaagaagc aaaattggtt
    26281 ttctgagctc taatagctct tttctcgata cattttgtcc ttcactgaat agtaatggag
    26341 ctatgataca tattacaaat atttctggat taaatgaaaa tgagaattta aatggcaaaa
    26401 tagacaatat aatccctagt acaatttgat atattgcaag aggaatatta ctccactttt
    26461 tgctaaatac agttgaaatc gttgtacaaa ccaataatcc caacaatgtt actaaaattg
    26521 acataactac ctctcttttc aataataata ttatagatta aacttatata tcaactaact
    26581 taatattaac agcatgaaat cccatcttaa tcaaaaaaat tttcagttta ttctattatt
    26641 cttattatat acatcaaatt taatttttga aatatctata ctagatgaca tgcaacaaaa
    26701 tgatttcttg atatttcttt taaaactgga atctcttttt tgcatatttc cttacaataa
    26761 gggcatcttg tatgaaactt acatccactt ggtggatttg ttggagatgg tatgtctcct
    26821 tttaatataa ttctattttt cttaattgta gggtcttgta ttggtactgc gctcataagc
    26881 gcttttgtat atggatgttg agaatgtaag taaagttctc ctttagttgc aatttctatt
    26941 atagaaccta gatacataac acaaactcta tgtgcaatat gatttactac actcaaatca
    27001 tgagatataa ataaatatga aagtttatat ttatcctgta aatccataag taaatttata
    27061 atttgagatt gaatagaaac atctagtgca gaaacaggtt catcacaaac tataaattct
    27121 ggatttaatg ctaatgctct tgctatacct attctctgtc tttgacctcc agaaaactca
    27181 cctggttttc tatttaaatg gtacttagaa agaccacaac tttctacaac atttaatata
    27241 ttatctttta tttcattctt tggcactaaa ttatgttcta taactgcctc ggatattatt
    27301 tgttccacat ttaatcttgg atttaatgaa ctatatgggt cttgaaatat catttgcatt
    27361 ttgggtctta actttctaag ttcttccttc tctaacttat atatatctac accattaaat
    27421 tcaactttac cttcagtttt ttcaattaat ctaagaatgg ttcttcctgc agttgatttt
    27481 ccacatcccg attctccaac tatagccaaa gtttctcctt tttttaaatc aaaagaaatt
    27541 ccatctactg cctttacaca tttaatttcc ttatcaaata atccagattt aacttcaaag
    27601 tactttttaa gaccttctac ttttaataaa ctctcttctt ttttcaatta tatctcctcc
    27661 tttttatata accaacatgc aatactatga ccactattca attctattaa aggaggtatc
    27721 tgagttctac atttatcaca tactttttca catctatcac taaaataaca tgaatctggc
    27781 atcccaattg gatttggtac ttgtcctgga atcgaataca atcttttatc tttgtttttt
    27841 ccaagagcag gctttgattt tagaagtcca atagtataag gatgttttgg atttttaaat
    27901 aattcaacaa cctctgccac ttctataatt ttaccagcat acatcactaa tactttgtca
    27961 cacatttctg caactactcc caaatcatgt gtaatcatca ttatagatgt gtttaatttt
    28021 tcttttatat ttttcattat atctaaaatc tgagcttgaa tagtaacatc taaagctgtt
    28081 gttggttcat cagctataag taattttgga ttacatgata aagccatagc tatcattatt
    28141 cgttgtctca taccacctga taattcatgt ggatatgaat tatatatttc ttcagctctt
    28201 ggtatttcta ccagttttat catatcgatt acctttttct tagcttcttc ctttgataaa
    28261 tcttgatgta tcataatacc ttcaatgatt tgacttccaa cagtaaacgc tgggtttaaa
    28321 gatgtcattg gttcttgaaa aatcattgat atctcattcc ctcttattcc cataagttca
    28381 tcttcattca tctttaaaat gtctttgcct cgaaagatta tttctccacc ttctattttt
    28441 gatgttggaa gtaacttcat caaagacatg gctgttacag acttaccaca tcctgattct
    28501 ccaactattc caatagtttc accttcattt atattaaaac ttacttcatt tacactcttt
    28561 acaatccctt tatctgtatg aaaataaact tttaaatctt taacctcaat tagtgcttta
    28621 ctcattattt tctcctctca gcaaaattac ttaagataac atacaccttt tgattgtatt
    28681 ttataaaaat ttaaattaca acattttctt acattttatt atttttttct attaattata
    28741 ataatataat agttaatttc tatatatatt gatttaataa tgattactat tatacaataa
    28801 aaaatatgtg ttaaatatta ttttatggag gttttataag aatatgaaat ttaaaaaatt
    28861 ggcatcatta atcttagttt catcattaat gcttacattt acagcctgtg cttccaatga
    28921 taaagacaag tctagtggaa gcaaatctgg tggaactgtt gtaataccta tggcaagtga
    28981 cccagatata atcaatggag cttttgcaaa cgttaaagaa gactctcttg catcaaatat
    29041 agtttatgct cctctttata cttatgaaaa aggaaattta gtaaattatt tagcagaaaa
    29101 agtagatttt aaagatagta aggaattaac tataaaatta aaatccaatt taaaatggca
    29161 tgatggaaag ccaataactg ctgaagatgt cttatttaca tttaatacag ttttagacga
    29221 aaaacaaaat tcaccatcaa gacaatactt attggtgggt gagaaaccag ttaaagtaga
    29281 aaaaattgat gatttaacag ttaagattac tttaccaact gcaagtgaat ctttcttata
    29341 tggtatatct aaaatttctc caataccaaa acatgtgttt gaaggagaat caaatatagc
    29401 taagtcagaa aaaaataata atccagttgg ttctggtgca ttcaagttta aggaatggaa
    29461 aaaaggtgaa agcatagtat ttgaaaagaa tgcagattat tttggtggag aacctaaggc
    29521 agattctata gcgctaaaaa taattcctaa tgaagcatct caagaagcag ctttaaataa
    29581 tggcgaaatt tcacttatga agacatctgc tgaaggatat gaaaaggcaa aatcaaactc
    29641 aaacttacaa acttatactt atagtgaaga aagattaaac tacattgtat ttaaccaaaa
    29701 tatatcaaac atggctaaca aagaagtaag acaagcctta tcatatgcct taaatagaaa
    29761 tgaaatgata gaatcagcat atggaaaaga aggttctgta cctgctaaat ctatattagt
    29821 tccagaagca gacttctata cagaagaagg tgttgaagga tatgaccaag atacaaataa
    29881 ggctaaagat ttattggata aaagtggtgt taaaattgat aaattaaaaa taggttataa
    29941 cacaggtaga tttgggcata aaaactatgc cttagtagct caacaagagt tgaaaaaaat
    30001 tggtatagaa gctgaaatag ttccttatga aagtaaggca ttcttcaata tactatttag
    30061 taatagcact gaatgtgata tgtatgtaaa tggttatgct tggggattag aaccaaaccc
    30121 atatagaggt atgtttgaaa ctggtcaata ttgcaaccaa actaaatatt ctaatgctga
    30181 aatagatgct ttatgggaaa aaggatttac tgagttaaac aaggaaaaac gtgaagaaat
    30241 atataagcaa atacaacagg atatatctaa ggatgcgcct atttacacta tagattatga
    30301 acaaaattta atggcagctc aaaaaaatct aaaaggaata aaagatgcta aaccatctcc
    30361 tgcaatatta tttgaagatt ggtctaaact gtatgtagaa taattgatgt aaaattttat
    30421 attattaatt agaaaaggag atgtctaaaa ataatattgt tttattattt taaaacgaga
    30481 catctctttt aattaggaga atttacttat gttaaaatta atattgaata gagtaaaagt
    30541 tttaatacct atgttaatct taatatctat attatcattt ggacttttag aattagctcc
    30601 tggtgaccca gcagatgcat atataaatcc tttaatgtct tcacaggata ttgaaaatat
    30661 aagggttaat atggggcttg ataaaccagt atatataaga tatttaaact ggttgaaaaa
    30721 cactttaagt ggaaacttag gtatttctta tataaaccat atgcccgtta cagaacaaat
    30781 tatggaaaaa atgggaaata catttattct tatgggtact tctttgatat tttcaatatt
    30841 ggtagctata cctcttggaa tatttttagc tgtaaataaa aattcaataa ctagtaaagt
    30901 atcatctgtt tttaattata taggtgtttc tataccttct ttttggatag gtatgatact
    30961 catttcaatt ttttcagtta aacttaacat tttcccatca ggaggtatgc atactattgg
    31021 aaatgatagt attggagatt tagtaaaaca tttagttttg cctgtaataa ctttaggtct
    31081 atataatact gctattttta caaattatgt agaagctagt gtaaatgaac aattaaaaaa
    31141 acaatatgtt attacagcaa gagcaaaagg attatcagaa aaagtcatac tatttaaaca
    31201 tgtcttgaaa aattcactga catcattggt tacaatccta ggcatgagta tacaaaaatt
    31261 agtcactggt gcatttgtta cagaagtagt tttctcttgg ccaggtatgg gaagattaat
    31321 gattgattca attttttcaa gagattatac tgtaattatg gcaataacta tgttatcagc
    31381 actattttta attttaggta atttagttgc tgatatacta tacttattaa ttgaccctaa
    31441 aataaaatct agcaaaggag gattctagtt gtgtttacaa gtatcttaaa aaaattaaaa
    31501 aaagataaat cctttgcttt tgcatgtatt actcttagca taataatcat attaattata
    31561 acttctttta tattaaatat agactctaat gcaattgata ctacttctaa acttcaagaa
    31621 cctagcttaa agcatatctt tggtacagac gaacttggta gagattactt ctctagagct
    31681 ctttatggtg gaaggatttc acttagtgtt ggtattttat caatgcttat ttctgtagta
    31741 tttggtactg ctgtaggtgt tattagtgga tatattggag gtaagctaga ccaattcttg
    31801 atgagattag tggatatgct tatgagtata ccaagtttct tagttttgat tgtattaaat
    31861 acttatctaa cacctaaagt ttccactatt atactaatta ttggattctt aagttggatg
    31921 gaagtttcaa gaattgtacg tggcgaaaca ttaaaaataa aggaaaatga atactgtcta
    31981 gctgctaaag ctactggtat caaaactaga aatatactat taagacatat agttttaaac
    32041 ttaaaagaga ctattgtagt tgctggttcg ttaaatattg ctaatgctat cttaactgaa
    32101 tctgctctta gttttttagg tcttggaatc caattgcctc ttgcatcatg gggtaatatg
    32161 cttcaagatg ctcaaagaca tatttttgac aagttatatt tagctgtatt tccaggatta
    32221 cttatatttt taacagtcat gagtttgact ataataagca aaaaattttc tagtaatact
    32281 taacaaattt tatatagaaa ataaaaacag tgattatcta aagatataca ctgtttttat
    32341 ttttcgttta aaaaaataca tattttgtat attataactt taaatactac tctaccatgt
    32401 tatgtaaagc ctattattgc tctcaaacat atagatgaaa aactaatagt ttattataat
    32461 aaaaattgat ttatttattt gttattttaa taacattttt aagagttctt tttactaaag
    32521 aaatctataa taaatttaaa tacatgttgg tctacatggc tataacgttc ttttttaggt
    32581 ctacatagta taactttcca tgtaatagga tgattaaaag gcttttctat aattttgcta
    32641 ttttcaaaaa aatcatgaac tggtgatggt agaattgtta taaaatcaga atttgtggtt
    32701 gatagcaaca aaaaatccca tgaaccagac attatatatc tttttaactt aacgttttca
    32761 tcagtaaatt tttgcattag cttatgatgt atcataaaag taggattaaa tattgcaagt
    32821 aactgctcat tcaaatcgtt ccaatctatc ttatccttct tagcaagtgg atgatttata
    32881 tttaaaaatg ctgtaagttc atcttcttga agcacatgtt cagttactac acttttatca
    32941 attttatgtg gatgtaacaa tactgcataa tctaattcct gtaagataag cattctattt
    33001 aagtcatatg cacccttctc tactatatca aattctatat ctggattatt tgcaactaac
    33061 tgtgcaacta catctgaaaa tgctattcca agtataagtg gaggtattcc tattctaact
    33121 tttcctttaa attgaactga atcttctctt aaatcttcca tcatattttt gtattcattt
    33181 atgatatgtt ctgcatttac ataaaatctt tctcctgctg gtgttaaccc atttaacctg
    33241 cctttatacc tttcaaataa atatacatct tcactttctt caaaattttt tataatttga
    33301 cttaatgctg gttgagatat gtgaagtgtc ttagatgcta ttgataaatt aaaaccagat
    33361 ttcactattt caacaaaata tttcaactgt tttatatcca ttttaaaccc ccttccccaa
    33421 aagtataatt tagattatat tttttcttct aatgatattt tataggaaaa tcaacataat
    33481 ttcattaata ttttaacaaa atatttttct aaatctacta taaattttta tcctataaat
    33541 ttttcttata cactatataa aaaattagac ttagacaaaa cgcttttttc gccgtaatct
    33601 ataattagaa acaggaaaac ctaaagcatt aggaataaaa gtaattgaaa tacttaaaaa
    33661 caagcttgta ttaaaaaagt actttattat agtgttccct aatttcttta ttcagaaaat
    33721 ttttaattat cattcaagaa aggtaactat tagatatgaa agatgtagta atcgtatcag
    33781 cagtcagaac acctatagga agttttggag gtgtatttaa aaatacttca gctgttcaac
    33841 ttggaactat cgctgttaaa gaagcgatat cacgtgtaag acttaattta tctgaaatag
    33901 atgaagtaat aattggaaat gttcttcaaa cagggcttgg acagaatgtt gctagacaaa
    33961 ttgctataaa tgcaggtata ccaaattctg ttccaagcta cacagtaaat aaattatgtg
    34021 gttcagggct taaaagtgtc cagctagctg cccaatcaat cacatctggt gaaaatgatg
    34081 tggtaattgc aggtggtact gaaaatatga gccaagctcc ttatatcgtt ccaactgctc
    34141 gctttggtag taaaatgggt gacataacca tggtagatag tatgctaaca gatggcctta
    34201 ttgatgcttt caatcagtat catatgggaa taactgcaga aaatattgca actaaatttg
    34261 aatttactag agaaatgcaa gataaattgg cattagaaag tcaaaataag gctgagaatg
    34321 ctataaaaaa caaccgcttt aaagaagaaa tcgttccagt agatatttta atacgtcgag
    34381 gaaaaataga aactattgat aaagatgaat atcctaaatt aggtatgaca tttgaaggtt
    34441 tagctaaact taaacctgca ttcaaaaaag atggtactgt aacagctgga aatgcctcag
    34501 gtattaatga tggtgcggct atgttaatac taatgagcca acaaaaagca gacgaactag
    34561 gtattagacc acttgctaaa ataaaatcct atgcttcagc tggtgttgaa cctgaagtca
    34621 tgggtactgg tccaatccct gcaactagaa aagcactgaa aaaagctgga cttagcatca
    34681 atgatataga cttaatagaa gcaaacgaag cgtttgctgc ccaagcatta gctgttaaaa
    34741 atgaacttca aatagattca tcaaagttaa atgttaatgg tggtgcaata gcattaggac
    34801 atccaatagg agcaagtgga gctagaatac ttgttacctt aatttatgaa atgcaaaaac
    34861 gtaaagttga aactggactt gcaacacttt gtattggtgg tggtcaagga atatcaatgg
    34921 tggtttcacg ataggaggaa atatgaataa aatagttagc attgatgaag ctctatctca
    34981 tgtaaaagat ggagatacaa taatggttgg tggattcatg gccaatggtt caccagaaaa
    35041 tttaattgat tatttatgta gtaaaaatat aaaagatttg acactaattt gcaatgatac
    35101 tggctttatt gacaaaggag ttggcaaaat ggttgtaaat aagcaattta aaaagataat
    35161 tgcttctcat gtaggattaa ataaagaaac tggaagacaa atgaacaaca atgaaacaga
    35221 tgttgagtta gttcctcagg gaactcttgc tgaacaaata cgtgcagctg gttatggtct
    35281 aggaggtata ctcacaccga ctggtattgg tactcttgtt gaggagggaa agcaaaaaat
    35341 tgttgtcgat agcaaagaat accttcttga aaaaccaatt tatgcagatg ttgctcttct
    35401 atttgcatca aaggtcgata aagcaggtaa tctagtttat aaaggctcta tgaacaattt
    35461 taataattta atggcatctg ctgccaaaat tacaatagtt gaagcagatg aaatcgttga
    35521 aattggtgat attgacccaa atgaagttaa tacaccaggt atttttgtga attacatcgt
    35581 tgaaggaggt aatttataat ggataaatta gaaatgcaag aatatattgc aaatagagta
    35641 tctaaagaat taaaagacgg agccgttgta aaccttggaa taggtctacc aaccaaagta
    35701 gcaaattata ttccggataa tgtaaacgtt atcttacaat ctgaaaatgg atttttaggt
    35761 ttaggttcag ccgaagatgg aaattcaagt gatgaaacta ttgtaaatgc aggtggtcaa
    35821 ccagttacta tacttcctgg aggatgtttc tttgatagtg ctacttcttt tggaataatc
    35881 agaggaggtc atgttgatat aactgttctt ggcgcacttc aggttgacaa atatggcaat
    35941 atagcaaact atatgattcc aggtaagatg gttccaggta tgggtggagc tatggaccta
    36001 gtaactggag ctaaaaaagt tattgtagca atggaacata cttcaaaagg ttctgcaaaa
    36061 atattaaata attgtactct tccacttact gcaacaaatg ctgtagattt gatagttact
    36121 gaaatgggag ttatggaagt aacatcagat ggaattttac ttaaaaaaat aaatccagct
    36181 ttcacattag atgatgttat ttctgctact gaagctcctt taatattgtc agattcatta
    36241 aatggaaaaa tatctgttgt ttaaaataat taaaactaga aaataaaaat ctcttagacg
    36301 tattacatat aaatttgtat tgcttaaaat attaatataa aaataaaata aaattggagg
    36361 aaatgaaaat ggttaaagat aaagttgtat tcgtaacagg agctgcaagt ggaataggta
    36421 aacaaatagc tgaatcgttc ttaaaaaatg gtgctaaagt aatgttctct gatataaatc
    36481 aagaatcttt aaataatacc ataacagagt tacaaaaaaa aggttatgat tgcatgagtg
    36541 taaagtgtga tgtaactaag gaagaagaaa ttaatcacgc tatagacaaa actgttgaaa
    36601 agtatggcag attagatata ttaataaaca atgctggact tcaacatgtt tctatgatag
    36661 aagatttccc aactgataaa tttgaattta tgataaaaat aatgctgaca gcacctttta
    36721 tagctactaa aaaagcattt cctataatga aaaaacaagg ctttggtcgt ataataaata
    36781 tggcctcaat caatggtgta ataggatttg ctggaaaggc tgcttataac tcagcaaaac
    36841 atggacttat tggactaaca aaagttgctg ctcttgaagc tgccgattct ggtataacag
    36901 ttaatgcttt atgccctgga tatgtagata ctccacttgt cagaggtcag ttcaatgacc
    36961 tttcaaaaac tcgtaatata ccattggaaa atgtcttaga ggaagtttta tacccacttg
    37021 taccacaaaa acgcctatta gatgttcaag aaattgccga ctatgctatg ttcttagcaa
    37081 gtgataatgc taagggtatt acaggacaag cttgcttact agatggagga tatactgcac
    37141 aatagttatt atatttaggg cttgaagaaa gtattcttca ggtccttcta ctaaggaggg
    37201 atatttatga gtattatcgc accaattggt ataattattg gtatcatagc tattatatat
    37261 ttttcagtta aagaaattca tataactata gctgctccaa tagcaacttt aatagttgtt
    37321 attttaaata aaatggatgt tgtaacgtca atgctaggtg ctgacaaaaa caattatatg
    37381 ggtgcacttg gaaactatat aatgagttat tttgcaatat tcttattagg ctcaatactt
    37441 gctaaattta tggaagaaag tggagctact gtatcaatag ctgattttat tttaaataaa
    37501 tttggctcta atcatcctta tagagtttta gttgctatat ttatagtaag ttttatatta
    37561 acgtatggtg gtataagcct atttgtagtt atgtttgcgg tcattccact tgcaagaact
    37621 ctatttaaaa aacttgatat ttcttggaac cttattcaga taccactttg gcttggtatt
    37681 gcaacaatta ctatgactat aattcctgga acaccagcca ttcaaaatgt tatacctatt
    37741 caatatctaa atacgtctct tactgcagct actataccaa gtattgtagg tagtataggt
    37801 tgtgctacat ttggacttgt ctatatgaaa tatgctctaa acaaaagtat tagaaaaggt
    37861 gaaacttatg caacttacac ttcagaatta gaagaagaat ctttagatag agaattacca
    37921 ggattttttg caagtatttt tcctctatta gcactcgtaa ttatagctat aactggaagt
    37981 acttttggtt cagaatttat gaaaaaaaat gtaatttaca tagctttaat agttgcaatt
    38041 cttttatctg cttttttatt ccataaattt ctatcatcta agatacagac attgagcata
    38101 ggtgctagtg gttctatagg tcctatattt gctacatctt ctgcagttgc atttggagca
    38161 gtaataatgg ctgcacctgg ttttgatgta tttagcaaac ttataatgtc aataccaggt
    38221 agtccactta taagtcttac tgttttgact tcaacaatgt cagcaataac agggtcctct
    38281 tctggtgcat taggaatagt aatgccaaac tttgcagatt attatttaag tactggtcta
    38341 aatccagaat taattcaccg tgttgctaca atagcctcaa atatactaac tatagttcca
    38401 caaagtggtg tttttcttac attcttaagc ttatctaagt taacacataa aactggattt
    38461 aaagaaactt tcattgtagt tgcagttggt tcactaatag cagaaattat tgtaattaca
    38521 ttaggattac taatgtatta atataaaaca aattaaaatt cataatatga aaggtggtgt
    38581 ctcaaagtga actttttact taatgagaca ctctttttat atgaaataac ataagcctat
    38641 catttcaaca ataaaaaaga accttatctc tattttgaga cagacccttt ttatttctta
    38701 aagatttaaa taattataat ttgttgctaa tacgaaagca tctactgaat aaatttaacc
    38761 aaataaaaaa aaatttatca taactataaa tttacgttat ggaattatta taaaaatatt
    38821 attttctcta actaagtaat tccatactat acaggctttt aatacaaatt gtcaaacaca
    38881 ctgaaatata cctatttatt ccatatatat cttttttaca tatctttaca gtaaaaaaag
    38941 tcttaatctt atgttagtat agattcacgg gtaaccccct acactaaagg ttacaaatca
    39001 gtaacatttt tagaattttt agattaagag gtgtttaatg atgagaagaa atataccaac
    39061 aaaattaaaa actatattag ctttaggagt gttaacttta gcaacagctt caagtgctgt
    39121 tactgcaagt gcttcatcac ttgaaaataa tgtaaatata agtaacaaaa gtttagtata
    39181 tagtaatact aaatacaagt tttctgcaaa accaaattgt tctgtaaaac cagaacttaa
    39241 gcctgataaa gataactctt gtaaggataa gaatcataat gacaagaatt gcaacaacac
    39301 taataaacct gaggacaaca ataattcaga ctctactaat aagcctgata acaataaacc
    39361 tgagtctaat aaacctgaag acaacaataa ttcaggctct actaataaac ctgataataa
    39421 taaacctgag tctaataaac ctgaagacaa caataattca ggctctactg ataaacctga
    39481 taacaataaa cctgagtcta ataaacctga agacaacaat aattcagact ctactaacaa
    39541 acctgatgat aataacaact caggctctac aagtgaaagt ttttcagctt accaaaaaga
    39601 agttgttgac ttagtaaata tagaaagagc taaagctgga ttaaatcctc ttactcttga
    39661 ttcttctata tcaaatgttg ctacaaaaaa atctcaagat atgattgata acaactattt
    39721 ttcacacaat tctccaactt atggttctcc atttgacatg ttgaaaaaat ttggaataag
    39781 ctataagaca gcaggtgaaa atatagctat gggtcaaaaa actcctaaag aagttgtaaa
    39841 cgcatggatg aattctgaag gtcacagaaa aaatataatg aatccaaatt tcagtaaaat
    39901 aggtgttgga gtagcacaaa aaagtggtgg ttcaatttat tggacacaaa tctttgtagg
    39961 ataaaatact ctttttccat acatatcaaa agagtatctc taagtgataa aaaacacaaa
    40021 tgagatactc ttttgtatct attttaaaat tatcaaatcc aaatatacat actataaata
    40081 ttatttgttc tcttaatttt agtgtgtaga gcaatatata aaaagtattg gcaagatgaa
    40141 aatggaaaac ttaaaaaaga tatttaatag gtaaaaacaa aatcttaata tcttcataaa
    40201 aaaaggctgt ctcaaaatag agataaggcc tttttcattg ttgaaatgat taaaatatat
    40261 ttgatttcat acaaaaagag tgcctcatga actaaaaagc tcactttgag acactctctt
    40321 ttatcaattc tatataagca ttataatact ttaatattat tcagctaatt ttttgtatcc
    40381 ttcatatctt tctttagcat cagcttcagc tttagcaaat aatccatcag cttcctctgg
    40441 gaattgttta gctatagcag aatatcttac ttgtgcaagt atgaaatctt tgaagcttgc
    40501 agttggttct tttgaatcta aagtgaatgg gtttttacct tcagctttta cttctggatt
    40561 gaatctgtat aaatgccagt atccgcaagc aacagcttgt gcttcattag ctacagttct
    40621 tcccatacct tcttttaatc catggtttat acatggagca taagctatta ttagagatgg
    40681 cccatcatgt ttttcagctt ctaaaacggc tttgataaat tgatttttat cagcacccat
    40741 agcaacttga gctacatata cgtttccata tgtcatagcc atcattccta aatctttctt
    40801 tctagatttt ttacctgaag cagcaaactt agccatagca gcagctggag tagctttaga
    40861 agcttgacct cctgtatttg aatatacttc tgtatcaaat acaagtacgt ttacatcttc
    40921 accagatgca agaacatggt ctaatccacc ataaccgata tcatatgccc aaccgtctcc
    40981 acctagtatc cattgagatc tcttaactag ataatctttt ctatctttta tagcttttac
    41041 taattcttta gctttttcat ctgtacaatc acaagctggt ttttctaata attcaacaac
    41101 cttagcactt gctactttag aagcttctcc atcatttcta gaatctaacc actctgtaaa
    41161 tactgcttta gcatcttcac agatatccat agaaactaac tctgtcatag catctactat
    41221 tttatttctt atttgcttaa cagctgtgta cataccaaat ccatattcag cattatcctc
    41281 gaataaagaa tttgcccaag atggaccttt accttcatgg ttacaagtat aaggaattga
    41341 tggtgcagaa gctccccata tagaagaaca tccagtagca ttagctatca tcattctatc
    41401 tccatataat tgtgtaacta ctttaatgta cgcagtttct ccacatccag cacatgctcc
    41461 tgaaaactcc attaatggtt gtctaaattg acttccttta acagtatttg caggcataac
    41521 atcaactttt ggagctactt catcaggatt tactgcaaat gcccaattat ctaactctgt
    41581 atcaagttga gtatctattg gcttcataac taaagctttt tctttagctg gacatatgtc
    41641 agcacagttt ccacatcctg tacagtcaag tggactaact tgaattctat attttaatcc
    41701 ttctaatcct ttacctaatg cttttttagc ttcaaatcct tctggagcat tagctaattc
    41761 ttcttcagta actaatactg gtcttataca tgcatgagga catataaaag aacattggtt
    41821 acattgaata cagttatcta ttatccattc aggaacatca acagctatac cacgtttttc
    41881 ataagctgca gtaccacaag ggaatgttcc atcttctata ccattgaatg tacttactgg
    41941 taagttgttt ccttcttgtg cagtcattgg tcttagtata ttttttataa attctggttc
    42001 tttaacttct cctttatctg catcaacagc attttcccaa gaagctggaa cttcaacttt
    42061 aactaaagca tttattcctg catcaacagc agcatagttc atattaacta ttttttcacc
    42121 ttttttacca taagctttaa ctatagaatc ttttaagtat ttaacagcat cttcttctgg
    42181 tattatttca gctaatttaa agaatgcaga ttgcattatc atgtttattc tgtttccaag
    42241 acctatttct tgtcctagtt taacagcgtt aactgtatag aattttatat catttttagc
    42301 tatatattgt ttcatatgag ctggtaaatg ttcttcaagt cctgcttggt cccatatagt
    42361 gttaagtaca aatgtaccac cttttttaag accttttaat aaatcatatt gataaacata
    42421 tgattgttta tgacaggcaa tataatcagc ttcatctatt aaataagtag ctcttattgg
    42481 agtatcacca aatcttaagt gagacatagt tataccacca gattttttag agtcataatc
    42541 aaagtaagct tgagcatatt tttcagtatg gtcaccgatt attttgatag cttgtttatt
    42601 agctccaaca gtaccgtcag aacctaatcc ccagaactta catcttatag ttcctttaga
    42661 agctattttt attggctcag atggagttaa agaagtatta gttacatcat caactatacc
    42721 tactgtaaat ccatttttag gttgttctga aactaagtta tcaaaaacag tttttatatc
    42781 tgaaggagta gtatcttttg aacctaatcc gtatcttcca cctattatca taggagcatt
    42841 ttcttttcca tagaatacat cacgtacatc taagtataat ggttcaccag ttgaacctgg
    42901 ctctttagtt ctatcaagta cacatattct ttcaacagta gatggcatag catctaagaa
    42961 atgtttcatt gagaatggtc tatataaatg aactttaact aaaccatatt tttctccctt
    43021 agcatttaag taatctatag tttcttctat tgtttctgtt actgaaccca tagctattaa
    43081 tacgtattta gcatcttctg ctccatagta atcaaataaa ctatgttttc tacctgttaa
    43141 attactcatt ttttccatgt atttttcaac tatacctact atattttgat agtatttatt
    43201 agaagcttct ctagtttgga aatatatatc tgggttttga gcagtaccac gagcaactgg
    43261 atgatttgga gataaagcat tatctctaaa tgcttgaact gcttcaaagt ttaataaact
    43321 tgcataatct tcattttcta gtaattcaac tttttggatt tcatgtgaag ttctgaatcc
    43381 atcaaagaaa tgtataaatg gtaatctacc ttctattgca gctaaatgtg caacaggtgc
    43441 tatatcagct acttcttgaa ctgaaccaga agctaataat acacatccag tttgtctagc
    43501 agccataacg tcttggtgat ccccaaatat tgataacgct tgagatgcta aagcacgagc
    43561 acttacatgg aatactcctg gtaataactc tcctgcaact ttatacatgt taggtatcat
    43621 taataataaa ccttgagatg ctgtaaaagt agaagttaaa gctcctcctt gtaaagaacc
    43681 gtggaatgtt cctgatgcac ctgcttctga ctgcatttca acaacattaa ctgtttgtcc
    43741 aaatatattt tttcttcctt gtgatgccca ctcatcaact acttcagcca tagttgaaga
    43801 tggtgtgatt ggatatatag ctgctacatc tgtaaacgca taggcaacat gagctgcagc
    43861 tgtatttcca tcaagtgttt tcataaactt agccataata tgtatcctcc ttgatagcta
    43921 caattgtact tgtttataac aagtctattt ttttatattt acatataatt tacacaacaa
    43981 aaatataaac acacatatag tcgcgcaaat actctatctt ctaatataat actacagatt
    44041 agataaaatt cctttgaaaa ttgtaaatta attacatatt taagtagatt ttttttaaaa
    44101 ttaaatatgt taagtttttt tcataatttt gtatacaata tataataaat taagttagac
    44161 ttttcaaact tttttatcta tttgtttata ttagtacaaa aagtaaaata attatcttta
    44221 ttatatgtaa atttttatat atataatttt accatattac atcatatttt tgtaagtttt
    44281 tattacattt tttataatac ccctttattt tataaattaa tctaatcgat taaatttatc
    44341 ctatagaaat ttattacatt taaaaatatg ctagcaaata aaatatctac tagcataaag
    44401 tctatttata atattaatca actattaatt ctactaaatc tccattttta acgcctgctg
    44461 catttccctc ttccatatca acatgcattt ctagattaaa gctgtcacta gctcttacta
    44521 atacattttc aaatactaat gctcttggtc cagatgtttt aacttttact atttgcttat
    44581 cagttacacc aaatttagca gcatcttcat tgctcatatg tatatgtcta gaagcaacta
    44641 taactccttc tttcatttca acttctccag caggtcctac taatttaaca cctggagttc
    44701 cagctatatc accagattct tttattggtg cttttactcc taatgggaaa ccatctgcta
    44761 aagatatttc aacttgagta tgagctctag ctggtcctag tactctaact ccttttaaag
    44821 ttccttttgg tcctactaaa tccacttttt cttcacaggc atattgccct ggttgagata
    44881 atggtttaaa tttagttaac tcatgacctg ctccaaataa tacgtctata tcagcttgac
    44941 tcaaatgaac atgtttatta gataatgcta ttggtaattt cattgaaatt ccctcctttt
    45001 aattatttaa attattgcaa tacaattata cataatatta cctacaaaat caaattatat
    45061 tgaatcaata tatatcttta aatttttata tattatatct ttaaattctt gattatctat
    45121 ataagataca tcatctgtat tgtttaaata tatattgtgt aaaagtttag tatttatact
    45181 cttaaagaaa aaatccataa caatctgtcc acctttaaac tgtgaatcat atgatttact
    45241 ccctcccact gcaatataca ttcctatccg tttcttactt ctatcaattg aaggtttatt
    45301 taatacatat ttacttgcat atatagcttg agttctatca acaacagtct ttaatttagc
    45361 tgatattgaa tcaaaatgta caggacttat tactatagtt ccttttgaat catcaaacat
    45421 tttatataat ggcttcatat catcattaaa cttgcaatac cctgtttttt cacaaaatcc
    45481 acatgctgtg caatattcaa tatccatatt gtagatatta aatattttac aacttaatcc
    45541 acactcttct agcatagctt ttacttcatt tgctataaaa ctgcaatttt tattaattct
    45601 tggacttcca tttattatta taatctctgg acttttaagt ccttttttat cgtttgaatc
    45661 cataatcaat ctacctatcc tttaacataa ctattaaaat atcatcatca taattatctt
    45721 ttgatgagaa gttctttaaa tccaatttta gattttctac tattaactgt tgatttaatc
    45781 taaaattttc ttttagaaag ttttctaatc ggtttatacc atactcttct tttgatgaat
    45841 tttttatctc taaaattcca tcagtgtaca tacataccat tgcataatct tccattgaaa
    45901 gagtattatt ttcataagta gcatcttcca ttattccaat aggtatgccc tttttacaat
    45961 ttaaattatt ttctattgtg ccatctttct ttacaactac aggactatag tgacctgcat
    46021 tagacacagt aatcatattt tctttagtat ctataatacc tatcaaacaa gttgtaaata
    46081 ctcccatctt gtcaaattca tcatataaca tcctatttaa atttgtcatt atttctcttg
    46141 gtgtattgta ttgattacat aatactttaa aagcaccttt tatcattgca acaacataat
    46201 ttgataccat tccatgtccc attacatcag caacaatata aactatatgt tcatcatcca
    46261 cttttgtagc ataataaaaa tctccaccta ctacccttgc tggtttatgg taaaaataaa
    46321 gttctttctc atcattacaa tccattttac ctttatccat aataagctgt tgttgtttat
    46381 ttaatatgtt caactcgttt tcaagaactt tgtgttttac ttccttttca ttatgttcat
    46441 ataattgcat accaacagaa acttgtttgg acaatatact aagaatattt aaatcatcac
    46501 tagtatagtt tttgctattt gcacaaacta tacatcctat agtatcattt ttatcctgaa
    46561 gtttgtaata tatataactt tccaaattta ttcccttttt tgagcatggt tttaaattat
    46621 ttagataccc ccccaatgac tctttcttta aaaacatatc aatcatagga tataaatttg
    46681 ttttcttttt ttgctcaaca ttataacaac taactatagc atccttatta tgatgactat
    46741 ttataattat taaagcttct ttactattag taatattaca cgcttgttta cttaaatttt
    46801 ccaaaaagcc atcaatactc ttattaaggt atagtttttt agtagcttca tttatggcac
    46861 ttattgagcc tcttagttta tcaattaaaa tttgtacatt attattttta ttagttattt
    46921 ctattgataa acttaagagt gatgcaacag atgatataaa atctatatct tgttctgtta
    46981 tataatcatc ctcttttaaa aaacaagtca taaatccaac aactacgtta tttattataa
    47041 aaggaaaaac tattctccca atataacctt cagcttttgc taaatctcgt tcaccttctg
    47101 ctctagaatc ttcaaataca ttttccacat atattatctt tttttcattt acagcctcat
    47161 gtatatactc tggatatgtg tcaaaatcaa tttttgcacc tttaagacta tttattggaa
    47221 aaatttgagg tacatattct aaggtttgcg aacatactaa gtaggcatat ttagaatcat
    47281 ttttataaaa tagattcaca catgcctttg tagggtgaac aacttctaac attttttcta
    47341 ttatgtcatc ttttatatta aaaaaatctg tagattcagt caccatagcg gatatttcca
    47401 ctaaattctt cattttataa ctgccattag tcatactcaa tttctccccc tatatattaa
    47461 actatttatt agtattttaa catacattct ttatagtaaa aacaaaaaca acgacaaaac
    47521 tagcaactaa tatccagtta aatcctgtca ataatcctat aagaagtaat gtaattaatg
    47581 catttagacc tgttctccct ttttcatcca taaaagttat tttagagatg tcgatattat
    47641 ttttcacatt atttatatgt tcttttactt gttcttccat atttctttta tatagtatat
    47701 aaactacacc aactaaaaac actcctatac ctaaaaccat gtttaaatta aaagctacca
    47761 taagcaatat tgaaccaaaa gctaacttta aaagcttaaa aaatacacta tctatcttta
    47821 gagttatatt tctgatttct ttacattcat ctcttaaaga atatccactt gaatctaagt
    47881 tattaatctt tgtttttaca atttcagaat aaaaaagctt tattatccca tttaacattt
    47941 tcatcactcc taaatatttt acatatattt gtaagtttta caattataat attttttata
    48001 cttggtaata ataaaaaata acaaatattt tatgatttag tatttattgt aaataagtta
    48061 ttgaagcttt attagttaag tatagatttt taataaatat ttttataatt agaaggaagt
    48121 ggttttgtgc aaatagaaca actcgattta gaaacgagaa ataaaatata ctgctatact
    48181 aaaaaaattc ttagaaaata tcaaaaaggc ataacatctg gaaaattaac agcagataaa
    48241 tttgcagata atatattgtc tcaagatttt atcagtagta tacttaatga aaaaatagtc
    48301 aatgagacaa actttaagat atcttataga aactatatag aaactttaat aaatattcaa
    48361 aatgaaaatt tatctaactt aagaaaaaaa tctactaaaa ctgcaaaatg tttcaatatc
    48421 tctcaaataa cccaattaaa aaacttacta agtaatactg gttataattt attaattcca
    48481 tataaatact taactgctag agatatagaa ggaatagtta ctcttattaa tactgggtct
    48541 attgaacttg gaaatgagag aatctataat tatatatcaa aagcataata atttaaacct
    48601 attataaaaa ggcaatttat ttaataaaaa atatttttaa tattaaataa attgcctttt
    48661 taattttgga ttaaatactg ctatccaagt gctttttcta gtttagataa atgttgtatt
    48721 ggataatatt ttagtagctc tttaaattgt tctactgata aaccttctgt tgttgtatat
    48781 attcttattt tttcatctaa gtcactatta ataaatttgt tttttaaatc ctcaaaattt
    48841 acactactca ttttacatca ctccttatgt aacatattca tttgtattat tttatccaaa
    48901 tacataaaat ttattacata aaaatatatc tatttaataa tttaataaaa aaaagctatc
    48961 tcaatttaga aattactttc tatatttgag atagctctag ctaaactaat agccaatagt
    49021 tataaataat tatttataac tatctgttgc tttttccagc catttgttgt tcagccattt
    49081 caactaattt tttagtcatg tatccaccta cataaccatt ttctcttgca gtaaggtttc
    49141 ctttatcaac ttgttgataa ttactcatac caagttcatt agctatttct aatttcattt
    49201 ggtttaaagc ctcttttgct cctggtacta ctgttctgtt gttgttattg ttacttgcca
    49261 tgttgattac cttccttctg tttaatttat tttgtgttgc tgataatatt atgtgatttt
    49321 aaacattttt aattcaggta atttttagca attaaaacct gccaaatatc cagtagaaaa
    49381 agctatttgc aaattgaatc cacctgtata tgcatctata tctattactt ctccagcaaa
    49441 aaataaacct tctactaatt ttgattccat tgtacttgaa tttatttcgt ttacttttac
    49501 accaccagaa gttattatag cttcttcaat aggtctgtat ctctttactg taaactttaa
    49561 attttttaat aaatggacta agttttttct ctgctctcta gaaatttggt gaacaaccgt
    49621 atttggatta atttcactta agtttattat tataggtatt agtttttttg gaagtaaatc
    49681 atctaatgct ttttcaaatt ttttatttgt atacttctga aaatcttttt gaactctttt
    49741 atccaatttt tcctcatcta atgcaggttt taaatctaat aatatagtgt aattttcttt
    49801 ccttgtatct ttcattctac aacttgcaga cttgattata ggtccatcta aaccaaattt
    49861 agtaaattca agttctccaa aatcactata aactttcttt tgtttgctat taaacaccct
    49921 tatttcaaca tttctcaatg ataatttttc taaatctttt gtaaaacttt cttgtacttc
    49981 tataccaatt aaagatggtt ttgtatctat aattgtgtgc ccctgtgaaa ttgcaaattt
    50041 atatccatca ccagtagaac cagtaagtgg ataacttaat ccaccagttg ctactacaac
    50101 tgaatcacac ttaatttctt ttttatcgtt taaaattact ttttcaattt tattattttt
    50161 agatattatt ttttctactt tcgaatttaa taatatattt actttcttac ttttaagttg
    50221 tctttcaaga gcatttacaa tatcaaatgc tttatcactc tctggaaaaa ccctttttcc
    50281 tctttctgtt ttagtcttta ctccaagatt attaaacata cttattacat catcatttgt
    50341 aaaagtataa aaagcactat aaagaaattt tccatttgta ggtacatttt ctattaattc
    50401 ctctatttca caagcgttag taatattaca tctacctttt cctgttattg caagtttttt
    50461 tcctaattta tgatttttct caattaatgt tacatcatag cctttctcac atgcagttga
    50521 tgcggctatc atccctgctg gtccaccacc tattattact accttcttca aataatcact
    50581 cttttctacc cttaattttt ttaatttaaa ctattgactc tataccttaa aatcaattta
    50641 attttaaaag tttaaataat gctggagctc ctgctattcc ataaaaaaca actactgtat
    50701 tcttaataaa attcataaaa attatactca tagtagagtc acttatattt aaagtgacta
    50761 ttaacattga aataaacaac ttaatgccaa tatataacat tcctattgtt actatcccaa
    50821 ccaaaaacct acatattctt gaaaaaatta tatttttact ccccttattt ttattactaa
    50881 aatttatttt tttgttatta ttatctgtat taaatttaac aaatctatcc tcaaccatat
    50941 atccaaatac aaatcctact attataccaa atttttcaaa atattcaggg ctatttagaa
    51001 atatagccac tattataaat ggtataagta aaactacaag taaaatataa tttttattat
    51061 catcaatata gtcaaatatt tttacaaata caactgtaaa aaatattcct aatatccaac
    51121 ccgctatcac atctattggc caatgaacag ctaaatacag cctagacaat cctactccta
    51181 aaaccataac tgcacctatt aaatgaagat atctcttttt aaatatagtc ataatactaa
    51241 cccaaaatgt tgttgcagtt tgagtatgag cacttggaaa agaataacca ccagcagtag
    51301 aaactctcaa tgattcaaga ccttttattc ctattggtct gggagctttt acaaactctt
    51361 tgatgcctaa atttacaaca aaatttccaa ttatagcaaa cagtattttc tgtccacatt
    51421 ttttatttat acaccagtat ataattgttg taattaatat taataacggt gcttctgtgc
    51481 ttattgtaaa gacaagaaat ataaagttca aaattggatt tcttaaattt tggaaaaata
    51541 ataatatatt taactgaata tccatataga tgtctccttt actaaaattt tatataatta
    51601 actaaaaagc actaactccc ttagaaatta gtgcttttga ttcacattta aatctattct
    51661 acatcctcaa ctatacctat ataaggtaaa tttctatact tttcagcata gtctattcca
    51721 taaccaacaa taaatttgtc ttctataaca aatccaacat agtctacagg tatattagct
    51781 tttcttctct gaggtttgtc aagtagagta catagtttta aagattttgg atttctagtt
    51841 tttaaagctg caactagatt acttaatgtt aaaccagaat ctattatatc ctctacaatc
    51901 aacacattct ttccttcaat atctacatct aaatctttta atattttaac tgttccagaa
    51961 gactctgttg agtttccata acttgtaaca ctcataaaat ctatatttac atccaaatct
    52021 atacatctta ttaaatctga aacaaataca ctagctcctt taagtatacc taccactagt
    52081 aaatcttcgc ctttaaagtc ttcttctatc ttttttccta attcataaac tttttctttt
    52141 atttgttctt ctgtcaacat ttttcctgta actttgtaca ttttgtatcc tcccgctttc
    52201 cttctattaa cttatatatt atagcatacc aaaatatcaa ttaaaatctt tttcatcatt
    52261 tttaaaccta aattaacagc ttatccatac ttttttgctt tttccattaa aaatttggaa
    52321 taacgattcc tttgtattat aatctatatg tacatatatt aatcagttat ttttatcgat
    52381 tatataattt tatttttgga ggtactttta tgagcacaaa ccatggagct aatttatata
    52441 gtttatctag taagtatggc ttttcaaagg aagaatttat ggattttagc tcaaatataa
    52501 atccttttgg tacatcaagt ttagccaaac aatacattgt aaacaatata gatatggtat
    52561 ctatgtatcc tgaccctgat tacatagact taaaaacttc tatatccaat tactgcaaat
    52621 gttctataga taatatagta cttggcagtg gagctacaga actaatttca tcatttatac
    52681 atacaattaa ccctaaacag gctcttttac tctctccagc ctattcagag tatgaaaaag
    52741 agttatctaa aattaattgt tctatagaaa aatactttgc aaaagaagaa gataattttc
    52801 atataaattt agaaaatctt ataaaaacta tcaatgctaa agattatgat ttggtagtaa
    52861 tctgtaatcc tagtaatcca actggatttg catttactaa agtagaagtg agagaaattt
    52921 taaaaaatac agactcattt ttaatgattg atgagactta tgtagagttt actgatacag
    52981 atacatactc ttgtactcaa ttagttgatg attattctaa tttattcgtt ataagaggta
    53041 catcaaaatt tttttcaacg cctggaataa ggcttggata tggtcttata tctaacacta
    53101 atgtcaaaaa tgaaataaat aaaaatctag atttatggaa tataaacata atagcttcta
    53161 aaatgggtga aatcatgttt agtgatttag attttatatc aaatactatt tccttaatga
    53221 acactgaaag agattacctg cttaaagagc ttaagaatat aaaaagctta gatatataca
    53281 atactaaagg caatttcata ttgtgtaaaa tcaagacaaa ggaattgact gctaaatctc
    53341 ttcgagaaca attgcttcca caaaaaataa taataagaga ttgttgttca tttgaaggtc
    53401 tagatgagta tttctttaga gtatgtatat taaaacctaa tgaaaataaa ttattaatat
    53461 catctcttaa agcaattttt aaataaaaaa atcactttat tttataattt tttataccat
    53521 aaaaaatgag ctatctctat ttgagataac tcatttttga ttattaaaaa actaagcact
    53581 ttgctcttct ttatttcctt ttttactcat tccatcattt attttatcta ctactactgc
    53641 tattgcatta tctcctgata cattacatgc tgtaccaaaa ctgtcttgag ttaagtataa
    53701 agctatcatt aaacttgcaa gcccaccatc tgatggtatt ccaaccattg gtaggaaagg
    53761 aagtgcactc attatagccc cacctggtgc tcctggtgct gcaaccattg ctatacctaa
    53821 cattgcaata taagggaata atacacctat tccatgtggc ataccattca tcattaaaac
    53881 tgcagttgta aatcctgtta ttgatatcat actacctgat aaatgtattg ttgcacaaag
    53941 aggtacaaca aattctctta tttgcttact tactccattt ttttctgcac aaccaacatt
    54001 aactggtata gttgctgctg aagattgagt tcctatagct gttaaatatc caggtacttg
    54061 attttttaac attcttactg gattttttcc tgaaaataca cctgctgctg tgaattgaca
    54121 tgccatatat acaaaatgta aaggtattac tactaagtat actttccaga atacccctaa
    54181 tattgtccat acttcccctg ctatagttat atttgcaaaa gttcctgcta tgtataaagg
    54241 taataaaggt ataatcactg ttgacaacgt tttcgttacg attccttgaa attcttggaa
    54301 gaaattaaat aaagtctctc cttttccatg atttcttaaa gctgatattc caagtcccat
    54361 tacaaatgca aatacgattg cagctgttac atctaccata ggctctaatg gaattgtgaa
    54421 tattggagct aacattcctg cctctgggtc tccaatttga cttgcaacac ttgcatcaat
    54481 aaaatttggg aagattaatg tagatactgc aaatgataat aatcctgcta ttaaggtaga
    54541 accatatgct attcccgtag ttatccccaa taactttcct gcacctgttg ctaaatcagc
    54601 tatacctgct gttacaaaac ctataattat aaatggtata acaaatttta ggaatgctga
    54661 gaataaagta ctaactgtta ctaatatttg tactattatt tctggtgcat aacttccgat
    54721 taaaataccg actatgatac cgatgattag tttgggcact aaacctattt ttttcttcat
    54781 aatatttacc ctccataact tttttaaatt aagcattctt gcacattgaa tacttagtta
    54841 aatattggat atccacttat tatgataata gagttttacc tctccgtcaa caatttaacc
    54901 attttttgtt aataaaaaaa agaataacat atataaattg ttattcttct tcatctttta
    54961 tagaaaattc tacattttca attatatttg aatgtttatc tttttttgcg ataactctat
    55021 acttattaat ttcttcattc actataccta tactccaaat taaaactata gcatcatcta
    55081 taaaaccaaa tgcccctgca atcatctctg gaattatatc aaccccagat acaaagtagg
    55141 atatagtaaa aattaataca gaaattacct ttattttatc aactatattg attcttttat
    55201 ctgtcataaa atcaggtagt ttaagtaaat caaatacaaa aaccaaacca aatttagcat
    55261 ttttaaatct gaccaaaatt ttttttaaaa tatctaaaac tatattaatg attattttta
    55321 aaaactccat atttatcacc tcttataatt ataggttatt ctacattata accgatataa
    55381 ttaatttgat gaacatatta gatataaatt ttatcaatta gtttatattt aaattataat
    55441 aattcaattc ctgcactact acaattttga atcatatcct gtggccatac tccaacttgt
    55501 acttctccta tatgagcttt ttgtaagaaa tacatacata ttcttgactg accaattcct
    55561 ccaccaattg tatacggaag ttcgccattt aaaagcatct tatgatagtc aaactctttt
    55621 ctctcttcac atccagcaag tttcaattgt ctatcaagag attcttcatc aactctaatt
    55681 cccattgaag ataattctaa tgctgaatct aaaacaggat tgtaaaataa tatatcccca
    55741 ttcaattccc aatcatcata atcagggctt cttccatcat gtttttctcc tgatattaat
    55801 gtttttccta tttgagttat aaatactgct ttcttctctt ttacaatttt atcttctctt
    55861 tcctttggtg ataattctgg atacatatct tctaactctt gtgatgttat aaaagttatt
    55921 tcttctggta atatttcttt tataccttca tacatactac atacaaaaat ttcagtatct
    55981 ttaaatactc catacaatct ttttacaaca tcttttagag tcttttcatt tctactactt
    56041 ttatctataa ccaattccca atcccattgg tctacataaa gagaatgtat attgtctaat
    56101 tcttcatttt ttcttattgc attcatatct gtatataaac cactaccaac ttcaaaccca
    56161 tattttttaa gggctaatct tttccatttt gcaagtgatt gaacaatttc tgcattttta
    56221 ttcataaatg gtatatcaaa gcttacagcc ttttctccat ttaaattatc atttgcacct
    56281 gtttctggca atacaaataa cggagaagaa actcttataa gatttaattc attacttagt
    56341 ctcatttcaa aaaaatcttt taattttttt atagctattt gtgtatcaat tacgttcaaa
    56401 ctacttttat aatcctttgg tattattgtg ctcatatcat tcactcccta tatttatata
    56461 tttttccatt aaaaaaaccc ttcatcccta tataaaaggg acgaaaggtt gcttttccgc
    56521 ggtaccaccc tgattagttt gaaataaatt caaacccagc tcacaatgta cagatattac
    56581 tcgatacacc ccaatatgat aacggtttgg ttccgactaa gcctactctt ttaaaatttc
    56641 ggttagcaac tccaggatgt tcttcattat agacttcata ttggtctcac accatcacca
    56701 actcgcttta attacattct ataactactc ttcctttcaa agtctttaag agtttaattc
    56761 aataaaattt agctctctta tgttttctat ttattcataa tagtatgata agtgattagt
    56821 taaaatttgt caagtttatt atttttttat ttttaaacac tttataaatt tttacctata
    56881 ctttataaat tttcaaatat agtctttaat ctgtccatac attcttttaa taaagactgt
    56941 ggacaagcta catttattct ttcaaatcct attccttctt ttccaaatat ataaccttca
    57001 tcaaataata cattagcttt tttaaacatt aaatcttcaa gttctacttc atttaatcca
    57061 taagcagtaa aatctaacca tgctagatat gttccttctg gataaacaag ttttactttt
    57121 ggtaggtttt cttttaagta ttcatcaata aattgtatat tattatctaa gtaatcattt
    57181 aattcattaa gccagtcttc actttcatta tatgctgcta ttgtagctac aatagcaaag
    57241 ggatttggtg aagttgcaac tccaacagct gtaacctctt cttgatattt cttttttagt
    57301 tcatcattat tcactattat attagacaac tgcattcctg ccagattaaa tgttttactt
    57361 ggagctatac atgtaactat tttatccttg tactctgggc ataatgactg taaaggagta
    57421 tgtttaaatc cttttctaat taaatcagaa tgtatctcat cagctattat ccataggtca
    57481 tattttttac atatttctac aacctttttt aattcatctt ctctccaaac tcttccaaca
    57541 gggttgtgag gactacaaag tatcagtact ttattgttgc tacacttagc tttttcttct
    57601 aaatcatcat aatccatcct ataagtacca ttctcatata tcagaggatt gtttacaaca
    57661 tccctattat tgctctttat cttagcttca aatgggtaat acactggttt ttggactatt
    57721 acaccttcat tagaattagt taatattctt actaaaatag caacagcagg tacaacacca
    57781 ggactaaaaa taatatcttt tctatttatt tcccagttaa atctcctttt ataccaatca
    57841 caaactgcat taaagtattt atcactatca tttgaactat atccaaatat tttatgttca
    57901 acttttttac gtaatgcttc tattattggt tctgcacatt caaaatccat atcagcaatc
    57961 caaaaaggca aaccatcttt ttgtgctttt gttgaacaat tagttctaaa atcccatttt
    58021 acacaatctg ttcctactct atctattact ctgtcaaaat tatacttact cattttttcc
    58081 ctcctaagaa taaaattttt ataattgtta agtctattac tagatgcact ttggtattat
    58141 tttacaattc ttaatattat aataatatca cttttttgtc caatagattt aaaatataat
    58201 tacttttcca tcatattttt tcaatattcc aaaatcagtt tcattatatc aaaataagat
    58261 tgctcatatt aattattttc taattaacat aagcaatctt tttctataac ttctataatt
    58321 ataattttaa atgtagaatt attagtttgg atgttatccc aatctctttt caaccaatct
    58381 acaccatata tctacaccat attctaaagc tttcgtatca aatgttacat ttggattatg
    58441 aaatcctggt gtagcatcag ctcctaatcc aatatatgta gttttacatt ttagtttttg
    58501 tgtataataa tggaaatctt ctccaccaga atttttaaat atacctatag atgcatcagc
    58561 tcccaataca tcttttatag tttcttcagc caagtttaca agttcttcat caaattctgc
    58621 tgctggtact ccatctttgg aaattagtac agcttctgca tttatagaac ttacagcagt
    58681 ttttacagca tgtattgctt tttcttttaa ttcttctatt atatcattac tttctgccct
    58741 tatatctaaa gccataaatg cttcatctgg tatattatta taagtttctc ccttaactga
    58801 tatatttgtt acttttatag aatttggtat agctggattt actcttattg catttattgc
    58861 attcactgct aaacagacag catctactgc atttactcct aaatgtggtt tagaaccatg
    58921 acaagtgata cctttaatcg taaattccat tatacagcta gcacaatggt acattgcagg
    58981 tatcgccttt cccaacctac aatcttgtcc aggtcttaaa tgcatcccta ccatttcttc
    59041 tacatcatcc aacaaaccag aatcaattac tcttaatgct ccatataatt tttcttcacc
    59101 tggttgaaaa agtattttta acttacctct ttttactaaa tttctctctt ttattaactt
    59161 tgcagctgtt aaaagcatag aagtatgacc atcatgacca caagcatgta tcattttctt
    59221 ttctccatct atgataaatt ccaatgcatc catatctgct cttatagcaa atactggtcc
    59281 ttctttttta gaatctatag ttactataat accagttcct gcaattcctt tattaatatt
    59341 ttcaaacccc attttttcaa gctcttttaa aacaaactct gatgtcttat attcttcaaa
    59401 tcctttttct ggtatagtgt gtagataatc atacacttta tgtacatact caatatagtc
    59461 tttgttagtt tccataatct agctcctcct taaaatttaa actttccatt tatattaaat
    59521 ctttattata attacctata ctacgaaata attcattata agcattgcaa tacaaccatt
    59581 taaagttgct ataacaaaca tatgtggata atatttagtt tttaactcag ctgttcctaa
    59641 aagtctcccc ataaattgta tctgcgctcc tgaaagcaat aacattggca atagaattgc
    59701 aacatgtgct tgacttattt gacctgctgt aaataaacct gcgattactc cagatgctcc
    59761 tcccattgat ataaatattg tcattatagc tgttgctgca acacctggaa gtccaaagat
    59821 actcattaca ggtgtaaata ttgtccctat tacatccaaa agacctgtta aattgaacac
    59881 agaaattata gcaaaaccga agatgacatt tggagccatt gaatttgtac ttatttggaa
    59941 tccatttcta gctcctccaa taaacgcatc tatcacattt ccttttttaa cttttacttc
    60001 tttttttgct acctcactca tgttaagctt cccccttgtc aaatttattt aaatataatc
    60061 taagtaagtt cgccccaaca aacttcataa ttaatattaa agcaagtggt ataaatattg
    60121 gtacatctcc tataaatgga aatagtgctg ctccagaagc aaagaaattc gtaattgcag
    60181 acgcacctga aaattgaaat gcacaaaata cagctttttg tttttcatct atatacccat
    60241 catctttaag ttctttagtc atagatgcag ctgcatctgt agactgtaaa ctagctatca
    60301 aagttagtcc tgcaaatcca ggtaacccta ataatggctt taatagtgga ctaagaagct
    60361 tttgagctgc cttcattcca tctaagtggt ctataactct aactactcca agtgcaaaca
    60421 taactgatgg tattaatgtc aatgcatata gccatccatc tcgagggcca gtacctccat
    60481 cacctctaaa gtttgcagct aatgtaccta caccatcatt aattgttccc aatgttccaa
    60541 aataacctaa tacattgtta aaatccaata cttttaatgg tcctggtgca tctttaaata
    60601 ttcctgaaaa aaataatgct cccaatatta atgctacata cccataccat tttacctcaa
    60661 ctttttctgt tgttgattct aatttactca tataaattct ccctcctgtt taaatgaatt
    60721 ttattagtat acttgtttaa taagcaaaac ttatgccaat accttaatga taaagccttt
    60781 gtatttattt ttttattgta gatttattcc agtgattaaa tagcttataa ttacttactt
    60841 ttcaagttta aaaatataat atttttttat taattattta aaatatacat tacaatataa
    60901 aaagctattt tccttatctt gcaaaaataa ttttaaaata ggtttttata ggataaaatc
    60961 ctaaaaaaac ctattattta atcaaatata taaatttata ctactatttg ttgtataata
    61021 taacttgcct tatactttaa atactttaaa aataaatact attttgttag gagaaataaa
    61081 atggataaaa agaagttaat ccttataaca catgataaat tgaatagtga ccactacaaa
    61141 gaagaattaa caaacttttt tggagacgaa ataatcatag aaacacaaaa tatacttgat
    61201 ggaataaaag aaaatttaga gggagatgta gtgctttctc tatcaccttt aactagtaat
    61261 tttttaataa aacattttaa ggaagatatt gaaattattc atggaacgaa agcactttca
    61321 aaacttggat atgaaaaaat gatgaaacta cctccaggta caaaatcgtt acttatgaca
    61381 accaataaaa cttcagcatt tgagatggct acttatctat ataaaattgg tataaatcat
    61441 atagattttg taccgacata tccagattgt gatgaaatat atgatttaga tacagctata
    61501 acaccaggtc agattagatt tataccaaaa tacataaaaa atattgttga tttaggttgg
    61561 agaaaaatct cattagatac atatatgtca ttattagtag tattaaaact aaaaaatgag
    61621 aaatttatag aaaaactata caaattgtcc aaagagacac taagtcatga tttcttaaat
    61681 acatcattag ataatatatc taaattaaaa actatacttt acatgaccat tgatgaaata
    61741 ggtgatggtc taatattttt caacaccttt aacaaggtta cttttgtaaa taagtcttta
    61801 cttaatatgt tgggactaga tgaaaaactt ataaaatcac cttccttaat ggaatatatg
    61861 ccaaatagtt tcttagataa aattacaaag aacttaaata tcgataatat gattatatac
    61921 atagacgaaa tagataaaaa attcatactt tctaaaaaac cattttatct ttataaaaat
    61981 atagagggtt gcttaattac attgaaagat gtaaacaata tagaaatatt agaacaaaaa
    62041 ataagaagtg attctgtcaa aagaggttat gtagctaaat ataaatttaa taatattatt
    62101 ggaaatagtt ctattataaa agattgtatt aaaaaggcaa aaaaaatggc tcttacagat
    62161 aatcctatat taataacagg tgaaacagga acaggtaaag aagcctttac tcagtcaata
    62221 cacaatcatt caaatagaaa aaataaacct tttgtagcaa taaattgtgc ttcattacca
    62281 agtgaattac ttgaaagtga actatttgga tatgaagatg gttcttttac tggagcaaaa
    62341 aaaggtggaa aaaaaggatt attcgaatta gctcacactg gcactatatt tttagatgag
    62401 ataggagata tgccacatga tttacaggtt aaacttttaa gagtcttaca agaaaaagaa
    62461 attagaaaaa ttggtggtac aagtattatt ccaattgatg tgagaatatt agctgccaca
    62521 aataaagatt tagagaaatt aattatagaa aataaattta ggatggattt attttatagg
    62581 ataagtatgt tcactttaga tttacctcct cttagaaaga gattagaaga tatacctctt
    62641 cttttagaat catttttaaa agaacttccc tataaaaaca taaaactcga taagagtctt
    62701 ttagaagctt taaattcata cacttggatg gggaatatta gagaactgag aaattgtatt
    62761 gaatatatgg cttacatggg ttccaattat cttacaataa atgatttacc tcaaaacata
    62821 tcttcaaaat taaataataa tcatatgtca agtaatatgt ccatattcaa tgatttaaac
    62881 caatatgaca aaaacatatg catttctatc ctaaaatcat tgcacatgaa accaatgggg
    62941 aggacaaagt taatgaaatt tatggaatat aatgtcacag aatacgaagt aagaaatatg
    63001 ctagaatatt tgacacgcaa tggatactta atttctagta aaggaagaaa aggctcatca
    63061 ataactgaga aaggcataaa aataatagaa aataatataa tttaaaccta tagtaattca
    63121 tctttacttt atcaaaattt atatataatt ataatttttt atttttctat aaatttttta
    63181 taaaataata aaacttattt atattaaaaa cgaagctatc tcaatatagg gataagcttc
    63241 tttttaattg ttaaaaagat aaaaatatat ttgatttaat agaaaaagag tgcctcataa
    63301 actaaaaagt tcattttgag acacttcctt aatattaata aattaattgg cttaaaatta
    63361 ctgattgata attataaagt acctctgtta aaggagtaaa ttctgaaccg attccaaatc
    63421 ttaattctgt aaacgcctct aaataatctg ggtcacatac atcttcttgt gttaaatctt
    63481 gtttcaccat ttcagtagca agataaattg ctactgatgg tagaaaagca tccattggca
    63541 taggatatcc accaagtcta tttgacatgc caaaagcctt agccttttca cttatcatgt
    63601 cgtttatttt atcataattt tgagcatctt tttcagatat ttttagaccc ataacactag
    63661 gatatgtttg tattggtgat ggattgcttt gctctgcaac tatatacttt aattctaaag
    63721 ccttagtcaa tataacttca tccatatact catttacacc aaatacattt atatcttttc
    63781 catatttttt aacttgtttt tcaatatctt catttaaaaa ctgcttaact ttgtttttgt
    63841 cttcttcagt atttatattt ggagtattaa cttgtacaaa tggcaatcct atatttttgc
    63901 aagtctcttt aatcatttct aatcttttgg ctatattaac atctttcaaa tcatctgtag
    63961 aggcataatg aataaaagct tttgctccca tttcttttga acgttcagct aatacttttc
    64021 ctctttcctc tgcactaacc cctagattca catcaacaaa ttggcttaat tggtttttat
    64081 catctcccat aggggcagaa atagttatta tttctggacg cttttccttt accttttgta
    64141 aagcaggtaa aagccctgct tggtcagttg aaactacaat agcctgaact tctttatcgt
    64201 cagccaactt tactatctta ttaatagcac tgtctatatt agaagtaaaa ttctctggca
    64261 atacaacatg ctttatagtt tgtttaactt tagtttgacc atctttgtct ttatcaattt
    64321 tattctcttc ctcatattct tttgccatct cttccaccat attgtattgt actttatttt
    64381 cagataatgg ttgagttacc actgcaactt taaaatcatc tataattttc tctttatctt
    64441 tcttgcctac aagtaaacta caacctgtaa gtccaataat taaacatatg gatagcataa
    64501 atgctgttat ttttctaagc attaattaaa acctcctaaa tttgtacatt cttatcacta
    64561 ttactaattt tatcatactt caaagaaaaa ttatattata aaaaattata tacatgctat
    64621 tataatccaa tctaaaaagt actatgggta gattaggttt agattaaacc tacccatagt
    64681 actaaaaatt aatatattat aaaattaaat taattacaaa ttattatgct acttgtttgt
    64741 tgctttttat aaagttacca actattccac ccaatatagc tggtattacc cagttaaagc
    64801 ccaaacctgc aagtggcaag ctttgtataa atggaacagc tcctgtcatt ccatttatag
    64861 ctgataataa actaatcact aatgtaacat atgctgcacc tttaaaagca ttatcattct
    64921 ttatatgttt accagccaac tccataacaa ctattgctaa taaaacagga tatatagttt
    64981 gtaggattgg aactgatatg gctattatag aatcaacacc taaatttgat ataagcatac
    65041 tagctacaca tatacctata accagttgtt tataactcgc cttacttatt ccttcaaaat
    65101 aagttgatat tgatgctgtt aaacctgtag aagtagtcag acaggcaagt gccacaatta
    65161 cacctaaaat tacctttcct acttgaccat ataacaatat actagttatt tcaactaata
    65221 aagatgcttg aggtatacta gtatcatata aagttgaaac tgttgcacct aaataagtaa
    65281 gaccaccata tactactgct agaaatacac aggctacaaa accagcttta atagtcattc
    65341 ctatttggtc tttatctgat gtatatcctt tagctattat agacatcaac attatagttg
    65401 ccatagatgc tgcacctaga gcgtccattg tttgataacc ttgtgatata ccttcaccaa
    65461 atagattttt atctatcata tgctcaggac tcataggtcc tgctggagaa attataccct
    65521 ttactattaa aacaaataat actaataata acgctggagt taaatactta cctattatgt
    65581 ctataacctt agaagaccta atagtaaatg ctaaagttag cccaaagaag attattgaaa
    65641 atacagcttt agctactgga ccatggtctc caaatatagg cataataccc atttcatatg
    65701 ttgttgcgca agttcttggt attacaagaa gtgctaaaca tgctagagca gctacactca
    65761 ttactcttgc taacccttta ccagctctcc taagtacatc atccatgctt cccttacatc
    65821 ttgctgttgc tataataaca agcattgcta taccagcatc tgcaacaaca aatccaataa
    65881 gaggaattaa ccagtctttg ccggattcca tccctaaaaa tggaggaaac ataagatttc
    65941 ctgcaccaaa gaacatagaa aataaagcaa aaccaactat aaaaatatct ctcatctgct
    66001 tactcacact aaattacccc ctttgaaaat attttgaata ttaagataat cttactttat
    66061 tttcagaaaa atgtcaatct ggtaaaaatt cacacattgt taacgttttc ccaatttatt
    66121 ttatgaatat ttttttcatt ttatgataat tcccatcaat ttcgttaaat caattgcttg
    66181 tgacacatca aaaattgctc ctctaatatc atctatacca atctcaattc cctcaatatt
    66241 attagtagta aaatctatgc cagaaagttt agttccatta aagtttgctt tttttaaatt
    66301 tgtatactca aatactagat tgtcaccttt tacttcttga aatacagaat tttcaaaatt
    66361 tgaatctaag atagaaacta tcttaaattt agagaatgaa aagttagaat acttacctaa
    66421 aacatccttg aacaaaacgt ttttaagtat acaatcatca aatctaactc ctatcaattt
    66481 actattttta aattcaactc tgtatatact tcctccatca aaaaatgtat ttgataaatt
    66541 gcagttttca aataccacat caagtaaatc aatctttttt aaattacaat tttcaaaaga
    66601 cacatttcta aatatacaag attcaaagct aactcttaca gaatctaaat cacttataac
    66661 ttcattttct ataattttat taaatatttt ttcatcatta aatatatctt caagtatatc
    66721 ttcaagattt tcaataattt ctaattctcc tgtaaattta ggtttttgca ctttaataat
    66781 tttagtcatt ttcccctcca ctataaatta tattattttt gatattattt tttttaatat
    66841 attaacttaa tatacatatt tgatatatta aaaagttttt acgaaatatc catgtattca
    66901 gtacttttat aattatatca caaactcatt tataataaac ttccattaat agtataaaac
    66961 aagttagaat aattgacatt atattcatac atattcaatt attggaaaaa gtaatttttt
    67021 tcaataaata accttaattt tttataaaat cgacaaaaaa acgaataatt ttattatatt
    67081 tattgtaaat aatacaatat tacgatataa tacaacagaa aaacaaatag aaaatgttca
    67141 tataatatta gtaatacggt ctaatagttt ctacaaactc accttaaatg ggttttctat
    67201 gaatagaatg tcaactctga atatatgttc tattctatct atatgaatat atattttttt
    67261 gtttatatct taaagttttt tggaggtaaa aatggtacca aacactaaca atgtacaacc
    67321 aggtggaaat aattcatcat taagcttttt agacaaaatg tttaaattta gcgaacgtgg
    67381 ctctagcctt aaaacagagg taataggtgg agttactaca tttttaacaa tggcatacat
    67441 agttttcgta aatccagcaa tattatctga agctggaatg gataaagccg cccttataac
    67501 agttaccata ctagctacag ccattggtac acttatattt gcgattttag gaaatgcacc
    67561 atttgctcta gctcctggta tgggattaaa tgcattcttc acatactctt tggttatagg
    67621 tgcaggaatc ccatggcaaa caggtcttgg ggtagttttt ctatctggat gtgtcttctt
    67681 tacactcgca gtaacaggtc ttcgaaagac aattgcagac gcaattccta gagaattggc
    67741 agtagcatct tcagcaggta taggtctttt ccttgctttt gttggtttaa aaaatatggg
    67801 tgttataata gctaatccag atactatagt ttcacttgca gattttacac caacagtttt
    67861 aatagctctt tttgctctta tcttaatggc aatactagaa tataaaaaag ttaaaggtgg
    67921 aatattgata agtatagtag ttgcaacaat aatatctgtt atatttggat ttgtggagtt
    67981 gccaacagca ataatctcga cacctcctag tatagcacca gttgctttta aattagatat
    68041 tgtaggtgca ttgaaattct cattaattgg acctatattc tctttcatgt ttatagacat
    68101 gtttgattca ttagcatttc ttattacttg ttgtaaacaa atgggtcttg aagatgagaa
    68161 tggtgaaata aaagatttag gaagaatgtt atatgcagat gtttcttcta ctttaatagg
    68221 ctcattctta ggaactagta ctgttacaac atttggagaa tctgcttctg gtattgcagc
    68281 aggtgcaaga acaggacttg cttcaatagt aacagcagca ttattcttag ctacacttat
    68341 atttacacca ttacttggta tagttcctac ttacgctgca gctcctgcac ttgtaatggt
    68401 tggtgtattc atgtttgaaa gcataaaaca gattgatttt gctgatagca aaatagctgt
    68461 acctgctttt gttacagttt tattgatgcc tttaacttat agtataagta ttggtttatg
    68521 ctttggattt atttcatata taataatgca tgtagtagct aaagaaactg ataaaatcag
    68581 tattacactt tggattatag gtttacttgc agttgtaaac ttagttctaa gttaatattt
    68641 ttaaatagtt ctaggtcaat attttaaatt atatagaata aaatcttatt tattttaaat
    68701 ttataaatag caaaatcact ttatatgtaa attgattttg ctattaactt tgtatagaaa
    68761 atttattatc ataatatata gatataaaaa tatatagaaa aggagtggtg tttatgtcac
    68821 aattacttat aaaaaatgca tatctaatta ctatgaatgc agatagagag gtatatgaaa
    68881 atggaagcat acttattgaa aataatatta taaaagaggt tggtaagttt gatattaata
    68941 ttgtagataa agatgcagag atatatgatg ctaaaggaaa aatactaatg ccagggctag
    69001 tcaatactca tgttcactta tctcaacaat taggacgtgg tcttgcagat gatgttgtac
    69061 ttttaacttg gcttagagaa agaatttggc catacgaaag tagcttcaat tatgaagatt
    69121 ccttgatttc atctactgca tgttgtgttg aattgataaa atctggtgtc actacatttt
    69181 tagaatctgg tggtcaatat gtagatgcta tggttgaagc tgttgataaa actggactta
    69241 gagcctgttt agctaaatca gttatggata caggagatgg tcttcctgag gcatggcaaa
    69301 aaactactga tgaggaaatc aacactcaat tagacttatt ccataagttt aataatacat
    69361 ctaatgagag aattaaaata tggtttgcat taagaactat ttttaataca actgaagaat
    69421 tacttgttag aactaaagaa ctagctgata aatacaatac aggtatacat atgcatattg
    69481 ctgaaatagc tgatgaaatt tcatttgtaa agcaaaacaa tggagttgga acagttgaat
    69541 accttgataa actaggtgta ttaggaccaa atcttttagc tgctcataca gtttggctta
    69601 ctcataagga aattgattta tttagattac atgatgtaaa agtttctcat gacccaggtt
    69661 cagctatgaa agtggttcta gggtttgcat caattcctga aatgttagaa aaaggaatac
    69721 ctgtttcaat tggtactgat ggagctcctt caaataatag aatggacctt atgagggaca
    69781 tgtatcttac atcactaata cataaaggaa gaactttaaa cccaactgtc gtaccagctg
    69841 aagaagtctt agaaatggct actataaatg gagcaaaatg tgctttacta gaaaaagaaa
    69901 ttggtagtct tgaagtagga aaaaaagctg atatgattat tctaaatcca gatacaattc
    69961 attgtttacc aatgcataat ccaattggaa atatagttta ttctatgact agtgaaaatg
    70021 tagactctac tatttgtgat ggtaaatggt tgatgaagga aagaaaagta ttagttgtag
    70081 atgaatctga attattaaat aaagttaaaa aacaagcttc taaaataaaa gataaagcag
    70141 aagttaaaat tccatctaaa tttaaaatta taaaataaca taaatttcaa ttgttttgta
    70201 aatatgaata tataaatata tttatataca tcatatatac aaaacaattg tttttttatt
    70261 ttatattcat tagcattgct cataaaatac accataattc cagaaaaaat aaccaatcag
    70321 ttaggattac atcatctaag caatatataa acgtcttagt ttataagttt tattctactc
    70381 ttatctaaaa ccatctttaa agagttttca actgtttcaa ttacatcttt ataactcaag
    70441 tacacatttg cgtagataca atcttcatat aaattatatt cagttactct acaatacagc
    70501 agttttcctc catctaataa attgctcaaa atatagtttt cttttcttgg gacatagcct
    70561 atcacatatc cagtttcacc aactatttta atagcttttt catcatatag attatctttc
    70621 tctctagtta aatagtatat cttacttttc tccaattctt tttcaagata ttctctattt
    70681 ctaaaccttg aaccagcaac agctattgaa gtcaaataga tatccttacc atgttcatct
    70741 acttttattt tttcaatatt tacaaattct ttttttaaat tattactttc atataatact
    70801 cttcttaatt catccttcaa tataacattt ttttcttttt catacgcttc tttaacaatt
    70861 tttaattctt caagagatag tttctctctg tatcttttta agatatttat ggattctttt
    70921 cttacttcat ttatatttcc aaatattcct tttaaagata tattctttcc ttcaggatat
    70981 agtgatttac ttcctttaag cacaataaaa aataacatat catcataata ttcttgagtc
    71041 aatttatctt tttctatatc tttcattttt ccaattaaat catctatttt aaacgtctct
    71101 tcaaaaaaat ttaataattt taatctagaa cttgtcgttc cctttttaga tatatgccaa
    71161 tatacctcaa aatctctaat atctctattt agatatggca acaagtcatc aaaagataag
    71221 tttacatcat aaatttctga catttttata atatcttctg tttttccact agcatctctt
    71281 aatgcttcaa aatatatatt tttccatttt tcactgagca agataccatt tatttctttt
    71341 tcaaattcaa tcttattgca ctctatattt tcatctttta agacgtttat agctattagg
    71401 aaaattgaat ataaactttc aaagttagtc ccataattta ctacagtagg taaaaatcga
    71461 ttcactaatt ctaatttatt acctatatat ttaaattcta cacttagcaa gtaattgcat
    71521 ataagacgtg ctaaattatt tatcttttct ttatctaaat ctcgtttttc aagatattca
    71581 ttcaaatcta caattgaaat aatatagttc atcaataatc gttcgtactt tgtatcctta
    71641 taaccatctt caattagata ggaatttatt ttagaatcta aattttccat attaacaata
    71701 gcaaatactt ttattgaacc tgtagccttt ttagataaat taaataaata tgtattgtaa
    71761 aattcaagtt ttttaatagt attactcaga taaaatacgt attctccaga tttagaaaat
    71821 gtatctacaa cttctcttaa gttttctaca tttagatatt tctcacttaa aactaaccct
    71881 agttttacat actcaacttt tttactctta tatattaatt ctttagttaa tttatataac
    71941 catttctcat caataatcat ttcttttacc aaagtattta tctttttcat aaaaatttcg
    72001 aaataacaaa ttaaagactt agaattacta ccctccaata aataaatttc attagattca
    72061 cttttataca tatttaaaat ttcatctact agattatttg caagctctac ttgttctctt
    72121 aacggtatac tttcatatat acataggtaa ttcatttcaa atgattcttt gtatccatca
    72181 taaccattaa attcctgcaa tcgcttaaaa acattctcca ccaaattatc ctcctttaca
    72241 tgatgtaatt tatattgatt atactactta tttcatcaag tttacattta tacgcaaaaa
    72301 aatattacta gaaattgaaa ttctagtaat atccttatta ttaattatgt aaatacccta
    72361 tcttataact gtaaataata tccttattaa ttttaattac ttttaataaa aatctatcct
    72421 agatttagac ttctctatgc atatattata ctaatattag acatcataat catacacttt
    72481 ttctttttca tatctcatgt ttacaaacaa tgcaagtaaa attccaacaa atgttattgc
    72541 cacattaaac aatacaacat attttggtcc attaactccc cctgattttg taataatacc
    72601 tgctatattt aaaataacat agtttgctat actagatgca atcataacta tagatgttat
    72661 ttttccttta ttagttggaa acatttcatt tgcagttgac gttgttaact gaagaacacc
    72721 tcctgctgct gaataaccta ttacaaatcc ccctaacata caaatagttg gagtttgaac
    72781 aaaatataca actaatagca taagtaaagc tattatagga tatataacta atattcttat
    72841 tggtttaata tattttttaa taagtaaaga agttattaat actgcacata ttactcccat
    72901 tgagtaaaag gattgaattt ttgtagggtc agccatccca tacaatttac ccaattcctg
    72961 attacaattt aaccataata caaatgtaga tgtacatgta aatccaatac aaacaagagc
    73021 tatacttgtt ggtgtaaatt tcatcttctc tgatttaact gttttgcctt tgttatcaat
    73081 aacattatta gctggtggaa atggtaacac agctatcaaa aaggcatcta ctacaataag
    73141 aatcgctgtt actataaaaa taactttaaa tgatattgaa tttgctgcaa caaatccaat
    73201 tataaaagga agcaaaaatt gtgctaaagc tattgtaaat ttagtaaaca tatttgctat
    73261 tgttcctaaa ctagcaaata tctccataca agaaggagtt acacaggtat ctaagaatga
    73321 atttgctgcc cctccaatta ttgcaaatac atatgctata taaaaatttg gtgaaaatac
    73381 aattccaaca aagaaaattg catgtagaaa gtttcctata agaccactta cttttcttcc
    73441 atacttatct gaaataggtc ctgcaaatgg ataagatata agtctcccta atcctagtgc
    73501 agcaataaca gctaaaacaa tacttacatc caatgttcca tctgataatg ttttagcccc
    73561 ccacattgat gaaaattctg gtttgtattg tcctaaaatt gaaattccaa taccaagtaa
    73621 aaaatatgaa aaatacaacg ctatggcagt aggataatat tttttattgt tcataatttt
    73681 tctcccattc ttgttaattt attaacattt ttgtgtacaa taaactcatc cccataatgg
    73741 atttaaagta tagtcagtta aaacttcaaa tttaactgga ctatgaggca aaacccacta
    73801 tgacaatttg taatttatta ttaaaaatat tgaaatacgt atgttttttc attgtttaag
    73861 atattactct tatctattta aagcataatt cattaacttc ttctacaggc atttctaatc
    73921 cagtatatag attaaaagct tctgctcctt ggtataataa cattcctaat ccaccaatag
    73981 ccttacatcc attagcttca gcatcttcaa tcatttttgt tttcttagga ttataaacga
    74041 catctgttac tactaaatct tttcttaaaa cactagtatc ttttatattt gtttcattgt
    74101 cataaggctt cataccaata agagttgcat ttgtcaatat atcacttgat tgtatctcct
    74161 cataaagttt gtttgtatct tctaaatcat atagattaac aacacattct ggtacatctt
    74221 tttttatatt ctcaactgtt tgctctgctc ttttataaaa atcatcctta ggattaaaga
    74281 tagatatttc tcttgctcca tctaaagcac attgtacttg tatagcagtt gctgcaccac
    74341 cagcacccat aattgtaatc tttttacctt taacttctac cccattttct tttaaattac
    74401 gaacatagcc tactccatct gttatatgac caactaactt tccattgtca tttacaattg
    74461 tattacatgc tccaattata cgagctgctg gagaaagttc atccatatat tctgtaacag
    74521 cagatttaca tggcatagta acattggctc cttttatatt aaaagttttt attgctaaaa
    74581 gtgcctcttt taccttatct actgtgatat caaaggctaa atatctataa tctaaatcta
    74641 atttcttaaa actataatta tacatcactg gtgatttaga gtgaccaacc ggtgtaccta
    74701 ttaatgcaaa taatcctgtt ctacctgaaa tttccataac tataatctcc tctcaatatt
    74761 atcattttct ttgcaaataa cctattcagt tatttacaaa gaaaaattta tttattattt
    74821 atatatttaa tgctacttca aaagcttctt ttgttgcatc taatgcccta cgagctttta
    74881 ctgcatcacc aacaatataa gtttctttta caacttcttt tataacttca cttaatgggt
    74941 cgtaattacg agaacccata gcaagtacta ctgaatcaaa accttttaaa tcatgctctg
    75001 tgccatctgt aagtaaatag tcgacaccat catcataaaa tttagaaact tttgcaccag
    75061 taatggtatg tatattataa ttttcaaaat cattcataag gaatttacgg tgttctgaaa
    75121 ttacatctgc accaacttct tctctaagct caataacagt aacctcatgt tcttgttctc
    75181 caaggaaggc tgccacttca catccaacca ttccacctcc aacaacaagt acttttttcc
    75241 cacaagattt tttaccatca agaaggtcta cagcatgaat taatcctgaa tcattgattc
    75301 ctggtatagg aagtacaagt ggagttgctc cagtagctat aataactaca tcaggtgatt
    75361 tctcctttat aagttctact gtagcttctg tattagtaca tatttttaca ccatattgat
    75421 tacattttga aatataactt ctaactaagt tagtaatatc tcctttccct ggaggatatg
    75481 cagctaaacg catttgccca ccaagaactt cagatttttc aactaaagtt acattatgtc
    75541 ctcttttagc acatacccat gctgcaagca tacctccaac acctccacca acaacaagaa
    75601 cttctttgct aatttcagct ggcttaagct ctgcttcatg tcctaatata ggatttgcaa
    75661 gacaggctat aggtttgcct tggaacatat ttggtacaca tccttgtaag catgcaatac
    75721 atggtatcat ttcgtctagt tttccatttt tagctttatt aggcatttct gggtcagcca
    75781 agctttgtct accgaatgct actaaatcac atcttccttg tctgaccatt aactcagcaa
    75841 actgtggctc tgtataacgt ccaactgtta ttactggtat actaacagct ttttttattt
    75901 cagtaactaa ttctgcactg aatccagcat gtaaagtcgt aggagcccac atatattcat
    75961 catgtatatg tatactacgt gaaacatgaa gtccatctat tccacaatct tctagatatg
    76021 ctgctattac tgaactatcg tgtacatcaa ttcctcctgg aacatcatca gtgctgttta
    76081 tacgacataa tattgctaga gaatttccta cttttttacg tatattttca atgataagac
    76141 gaggtaatct cattctattt tcaaaacatc caccaaactc atctacacgc ttatttgttc
    76201 tttgtgatat aaaactacta accagatatc catgagcgca gtgaacttca acagcatctg
    76261 caccagcttt ttgtgcacgt aatgcagcat ctccataaga ttctataagt tcatatattt
    76321 cttctgttgt tattgctagt ggtgtattac gaccatttga tgcaggtatt gcagaagcag
    76381 cttttaatgg atgtcctgct actttttcat ttccttcaga accagcatgt tgtaattgta
    76441 tagatacctt cgaacctgca tcatgacaag catctattac atttttaaaa ctatctattg
    76501 tgctatcatc atataaacaa ggcttacgag gacctccttt tgcttttttg tctacaacac
    76561 tagattctat agttattaat ccaaaaccac ctaaagcacg ctcaagataa taagccttag
    76621 aagtttcact taaagtacca tctgtgtttg caaaattgtt tcccatagga ggaactacaa
    76681 aacgatttgg aactgtcatt gaaccaattc ttataggtgt aaacatagaa ctaatcttca
    76741 taaggttttc cttcctttca agttttatat ttaaaagttt tatattataa tactctgtat
    76801 atttttatat tatataagtt tattttggag atacttcaga ccatgcttta tccaaaactt
    76861 ttttagttgc attaaataca gtttcagcag cagtggataa ccctttagat attagagaat
    76921 cagatattac ttctgcccct atgactcttg gtgatatatt gtgttctttt attattttca
    76981 caaattcctc tgtgtttcca tatccttcac ctggagccaa acggtcatgt agtgattcat
    77041 ccctaagttt ttcataagga gtttctaaaa catcacacac ttgaattgat actatcttat
    77101 ctgctggaac aggtgcaagg tcctctgctg tctgctttgc tctagcccag tgccaagtat
    77161 ctaatataag cattgcattt tcacgattac atcctttcac aacacgccat gcagtagcca
    77221 aatctggtac accactatat ggcataaact caagacctat aataagttca cctgctctgt
    77281 cacataattc tcccaatgca gttataattt gttcttctgg tatcatctct aataatccac
    77341 aatttatatg tttaacatta aataagtgag ccatatgata aacattttgc tctttttctc
    77401 tttgagcttc cgttctatct tcttctcttc cccattgagt tatatattct acttctgtaa
    77461 ctttcatatc atattcatta agaattgcaa tcatttcttc atctgtaaca cctgcatttt
    77521 ttgcatcaat ataattttca gcacgcaatc caataccatc aaacccagct tcttttgcaa
    77581 tacgaactct atcacggaat gaaacttctg ttcccaatgt ataagaacta attgtaattg
    77641 gaatattaag atttttttga ttattcatag cttaaatttg ccccctttat
//
LOCUS       DAGSRM010000009        67854 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.009, whole genome shotgun sequence.
ACCESSION   DAGSRM010000009 DAGSRM010000000
VERSION     DAGSRM010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 67854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67854
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.009"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(183..1463)
                     /locus_tag="MJE33_000662"
     CDS             complement(183..1463)
                     /locus_tag="MJE33_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBY9646631.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNLAEILANEPNEFKLLLKKYLDDGLLFPTARANALFDYIHQDKKNNFI
                     QNYSKEQLSKTLNSSNNILGIEYIKNLIHLNSSIKPFTISRVQSEYNSEEIESDICSA
                     TAIRTSLKENTDLFHLKNVIPEKTYNILNEKIDNGFLPMFDDFFFDILKTIILRDMDT
                     LKEYFEVNEGIENRIYKDIFTSEFLHELQLAIKSKRYTLTKIKRTLNNILLGIKKSDV
                     QLIKNIDTIPYIRILAFNDKGREILKEIKNKSEIKIINKFSNISFSLDDTIFKTLISY
                     DIKATNIYNSIYYKNNKDLVKGPMDFYTHPIYVK"
     gene            1926..3122
                     /locus_tag="MJE33_000663"
     CDS             1926..3122
                     /locus_tag="MJE33_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBY9646632.1"
                     /translation="MKILVLNCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHE
                     KAGRDDKYVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFAT
                     SVVITPEVEEALKECIDLAPLHNPANIMGIDACKAILPDVPMVGVFDTAFHQTMPKSS
                     YLYGLPHELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAA
                     VDGGKCVDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVY
                     GMTGISSDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVG
                     ENGKAIRADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARD
                     TLRLVK"
     gene            3215..3739
                     /locus_tag="MJE33_000664"
     CDS             3215..3739
                     /locus_tag="MJE33_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBY9646633.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDNCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            3764..3940
                     /gene="rpmF"
                     /locus_tag="MJE33_000665"
     CDS             3764..3940
                     /gene="rpmF"
                     /locus_tag="MJE33_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBY9646634.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            4147..4704
                     /gene="fapR"
                     /locus_tag="MJE33_000666"
     CDS             4147..4704
                     /gene="fapR"
                     /locus_tag="MJE33_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HBY9646635.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            4715..5737
                     /gene="plsX"
                     /locus_tag="MJE33_000667"
     CDS             4715..5737
                     /gene="plsX"
                     /locus_tag="MJE33_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBY9646636.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVALNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDINQFISKYNEEN
                     KNNEDE"
     gene            5763..6749
                     /locus_tag="MJE33_000668"
     CDS             5763..6749
                     /locus_tag="MJE33_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBY9646637.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVNEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            6858..7787
                     /gene="fabK"
                     /locus_tag="MJE33_000669"
     CDS             6858..7787
                     /gene="fabK"
                     /locus_tag="MJE33_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HBY9646638.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            7803..8753
                     /gene="fabD"
                     /locus_tag="MJE33_000670"
     CDS             7803..8753
                     /gene="fabD"
                     /locus_tag="MJE33_000670"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBY9646639.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNTVAKSVIDEASDALNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAQIDLKYNACLGLSLGEYSALVAA
                     DAIDFKDAVCLVKKRGKFMQETVPAGVGAMAAILGLDRNVLENVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAMVKCKEAGAKRAVKLNVSGPFHSSMLKEAGVKLASELEKV
                     NITKPKVDVVANVNADYYKNEGKDLLIKQVSSSVLWEDSIERLLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            8753..9502
                     /gene="fabG"
                     /locus_tag="MJE33_000671"
     CDS             8753..9502
                     /gene="fabG"
                     /locus_tag="MJE33_000671"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBY9646640.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIESMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILVNNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTKVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMSSYITGQVINVDGGMVMQ"
     gene            9556..9780
                     /gene="acpP"
                     /locus_tag="MJE33_000672"
     CDS             9556..9780
                     /gene="acpP"
                     /locus_tag="MJE33_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBY9646641.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            9809..11047
                     /gene="fabF"
                     /locus_tag="MJE33_000673"
     CDS             9809..11047
                     /gene="fabF"
                     /locus_tag="MJE33_000673"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBY9646642.1"
                     /translation="MNKRVVITGLGCVTPLGTGKEEFWSNIKSGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAIVSAKLAVEDANLDLDKVDRERFGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADIMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            11273..12991
                     /locus_tag="MJE33_000674"
     CDS             11273..12991
                     /locus_tag="MJE33_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBY9646643.1"
                     /translation="MNFRLPKKRLPKKIIFICIILISIILFIKYDQNGLKEREIINKG
                     KYMSESLVADKEYDEAKKVVKTSFESIPEKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDVKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASIFLKIGSYETGEKLIKDSFKIDIDEGEKNGMVRLYAIINLAEIYVEEGEY
                     DKAIEISSRIKDYKKFVNTDNYNDFDIMASIMQSNAYVGKNNIQKAKTFLEKADYLIK
                     SDRTVYILDKDMYYYMAMGNLEYKSENYNSAIDNYKKSLEISTKRKLTQEKIKNLNKI
                     LNIYEKQGNIESLNEYQKMLINEYKENKSIRDSETSFYIIDKVSNESKLFEKTKKEIK
                     YYKILFTVILIAILGSTFLYNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYREKDDNF
                     ALVMIDIDNFKLINDNYGHKFGDTVIKGITNTICVMLEQDDKVFRYGGEEFSVLIRNK
                     SGEEVADIIDSIRVAIANKKWNDDVTVTISAGVAHISDSKDVLEEADKNLYKAKQLGR
                     NKVVYK"
     gene            13022..15022
                     /locus_tag="MJE33_000675"
     CDS             13022..15022
                     /locus_tag="MJE33_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY9646644.1"
                     /translation="MKQSIAIVTDKITKLSSFLEENIRLVLGNYIDINHYYLEVLKEN
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKGKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLDNGVKDNYRQLFLKIEEQDVIL
                     NLSKDGIIFTSADGIINTFNKEALKILGINENPQGKNIEDIISEDLNILLEDKEIIDE
                     VVFTNKKYINVNKKSIFSMGNKAGIYYNLQEITYIKKLEQNLTNKLREQGQIARYTFK
                     DIKTKNKNMVKCVELAKKISKSDLSVLIIGESGTGKELLSQSIHNASNRSNQPFIAVN
                     CAAVPDSLLESQLFGYEKGSFTGALREGKKGLFELANNGTIFLDEIGDMPPLLQTKLL
                     RVLQEKQVMPIGSHNVINIDVRVIAATNKNLLKMIREGKFREDLYYRLNVLPIDVPSL
                     RNRKEDIITLMNFFLKNNIRLSPEVITILEKYNWPGNIRELQNVASYVSLMCDSMVFR
                     DDLPPYIRVECANNYEDGFGYTRKEFEEILRILYERRESSSGIGRGFIIEKLIQEGIE
                     ISESKVRKVLSYLSECGYISSKSGRGGSQITSEGIKLYNNIN"
     gene            15373..15618
                     /locus_tag="MJE33_000676"
     CDS             15373..15618
                     /locus_tag="MJE33_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBY9646645.1"
                     /translation="MKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGLKVLVEG
                     MNIEDVIQKLKGIECKTKPTSCPDQLSLALENYMNIK"
     gene            15653..16378
                     /locus_tag="MJE33_000677"
     CDS             15653..16378
                     /locus_tag="MJE33_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBY9646646.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNTDKEVIKKQIEMVDGVLISGGWDINPQLYGEETREETTFIYPEVDEFDLIAISI
                     ALELKKPILGVCRGLQILNVSLGGTLYQDNNLIEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGIIEAIEKEDENFVVGVQWHPE
                     MMVDYCDKMEKLFKYFINICSNL"
     gene            16400..17713
                     /locus_tag="MJE33_000678"
     CDS             16400..17713
                     /locus_tag="MJE33_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBY9646647.1"
                     /translation="MNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKSFGVASIFVY
                     IFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCISWGAL
                     IVGFPTSLSAVWAPAGEPHIQKIIIVAMIVGLTIINLLGVSLSKIVQNVITVGKLIPL
                     ILFIGIGIFFIKGVNFTSSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAEDMENP
                     QKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARICFGNMGAGLVT
                     FGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATMLITIPI
                     ALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLIAVCVG
                     TWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKIYGENLKKIV"
     gene            17919..18458
                     /locus_tag="MJE33_000679"
     CDS             17919..18458
                     /locus_tag="MJE33_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY9646648.1"
                     /translation="MPTITLKNGVDVLIREGVREDAQSIIDFYNEVGGETHFLSFGKD
                     EYKISLEEQENAIESAKASDNSVKLIAFIDGEIVGIATIDSNQKAKGKHVGVLGIVVK
                     EKYWGIGLGKRLMLDLIEWCKSNGITKKITLVTNEENYNAIGLYKKVGFEVESILKKE
                     CYYNGVYTDLIGMSLLLGI"
     gene            18547..20532
                     /locus_tag="MJE33_000680"
     CDS             18547..20532
                     /locus_tag="MJE33_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HBY9646649.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKVNDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTINLKGKTYKLKDTYLPTIDKKDPYKLTMEERNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDKMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIAEKET
                     HKENKNPYFKLRENEMACKRLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGIKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            20756..21724
                     /gene="spoIIIAA"
                     /locus_tag="MJE33_000681"
     CDS             20756..21724
                     /gene="spoIIIAA"
                     /locus_tag="MJE33_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HBY9646650.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKVSNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMTLDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKAIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGAYLGVPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIED
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            21714..22235
                     /locus_tag="MJE33_000682"
     CDS             21714..22235
                     /locus_tag="MJE33_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HBY9646651.1"
                     /translation="MQIKIIIIAFLIGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGGNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            22252..22449
                     /gene="spoIIIAC"
                     /locus_tag="MJE33_000683"
     CDS             22252..22449
                     /gene="spoIIIAC"
                     /locus_tag="MJE33_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HBY9646652.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            22465..22851
                     /gene="spoIIIAD"
                     /locus_tag="MJE33_000684"
     CDS             22465..22851
                     /gene="spoIIIAD"
                     /locus_tag="MJE33_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HBY9646653.1"
                     /translation="MEIMQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVM
                     FKLNFIVDSIQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEF
                     GGKIIVMSMSFPILLSIVEMVVNIIP"
     gene            22936..24141
                     /gene="spoIIIAE"
                     /locus_tag="MJE33_000685"
     CDS             22936..24141
                     /gene="spoIIIAE"
                     /locus_tag="MJE33_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HBY9646654.1"
                     /translation="MKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSEDKEYGETKN
                     SIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGLKI
                     LMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLIGF
                     KDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFFKN
                     FLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTVYLGLVSIQGLYVT
                     SFDKFSVKTAKFAIGNFIPVVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICLLP
                     VIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVAIL
                     TSISVVSQG"
     gene            24201..24818
                     /locus_tag="MJE33_000686"
     CDS             24201..24818
                     /locus_tag="MJE33_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HBY9646655.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGNYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNSAKSNNSSNEENKNNSTKREEENIN
                     SNDKENSKQVFKKGTEYGLNLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            24830..25384
                     /locus_tag="MJE33_000687"
     CDS             24830..25384
                     /locus_tag="MJE33_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HBY9646656.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKSDKQVGK
                     TETNKNAEKQVTKEQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            25411..26100
                     /locus_tag="MJE33_000688"
     CDS             25411..26100
                     /locus_tag="MJE33_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HBY9646657.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNTDESDSSKTEDSSNIDKQDGKESADIDIVDSKASKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKI
                     QEKELQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNSNK"
     gene            26210..26572
                     /locus_tag="MJE33_000689"
     CDS             26210..26572
                     /locus_tag="MJE33_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBY9646658.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            26657..27163
                     /gene="nusB"
                     /locus_tag="MJE33_000690"
     CDS             26657..27163
                     /gene="nusB"
                     /locus_tag="MJE33_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBY9646659.1"
                     /translation="MKKDRAQKSTTREYIMKLIYQININKEDFETLEDKVDNFLKDNS
                     EHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKHA
                     KNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGILG
                     SVVDEIGK"
     gene            27147..28217
                     /locus_tag="MJE33_000691"
     CDS             27147..28217
                     /locus_tag="MJE33_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HBY9646660.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVIFNEKIMLEVRDNSK
                     GLRQSEAVFQHINNLGILSDRIKSFKDKFNVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     KLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNKFISVHMSGGTTEILLTSKQDSHH
                     NVCDTNLGKIAKISIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGIKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIINDNGYNTNQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVNEVVFAGGVSASKYILRELSVKLRKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            28214..29419
                     /locus_tag="MJE33_000692"
     CDS             28214..29419
                     /locus_tag="MJE33_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBY9646661.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGVKIIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIKRR
                     YPKVNIKLYPVTVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RMSKSLTNQIELDQYRLETVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLKLE
                     DEKLVKIGALLHNLSPLATMDRGYSITQKNGKVINSIKGLKIKDSIDIVLKDGNLECM
                     IDKIENKEG"
     gene            29421..29636
                     /gene="xseB"
                     /locus_tag="MJE33_000693"
     CDS             29421..29636
                     /gene="xseB"
                     /locus_tag="MJE33_000693"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBY9646662.1"
                     /translation="MNLTYEEAYKRLESILSELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            29639..30526
                     /locus_tag="MJE33_000694"
     CDS             29639..30526
                     /locus_tag="MJE33_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBY9646663.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIEKEKLDYIHMNKTAAMMVGCMRAGATIGGANSEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDEAKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLKGLAEYIIDREY"
     gene            30556..30993
                     /locus_tag="MJE33_000695"
     CDS             30556..30993
                     /locus_tag="MJE33_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HBY9646664.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGIVTALILM"
     gene            31027..32892
                     /locus_tag="MJE33_000696"
     CDS             31027..32892
                     /locus_tag="MJE33_000696"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBY9646665.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KENESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYIDTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKELIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSAKVKSMSISVGEKLVDMANSNEN
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     REMELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDDGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEGMLKALLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNIDELYDFVGLSPKSIADKIRKLVIE"
     gene            33025..33837
                     /locus_tag="MJE33_000697"
     CDS             33025..33837
                     /locus_tag="MJE33_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="HBY9646666.1"
                     /translation="MKKRIDLLLVEQGYFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCSIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEDTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEVVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNEGYEFDGETYNNKIVEVVEASHNLDK"
     gene            33857..35536
                     /gene="recN"
                     /locus_tag="MJE33_000698"
     CDS             33857..35536
                     /gene="recN"
                     /locus_tag="MJE33_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBY9646667.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNRYLKKILEENDISMEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEEFKI
                     AYKNVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYGKIK
                     DRLDEILNRDERVEQLRSKLMNIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGPFTLDGVDDIEFMISFNLGEDIKPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            35750..36616
                     /locus_tag="MJE33_000699"
     CDS             35750..36616
                     /locus_tag="MJE33_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646668.1"
                     /translation="MNKKDTMHLIFTIVIFILLWYIVVAVSEINKNIESCSNKLDYLS
                     QEIMSTKSDISNTINEEMSKSYITKSIDLKVKKIEKKECVIDVDVQLSKLGKNGKVFF
                     MYKEDSDKWNETEMTKHGELSYACEIKINTGSEYDYKVVTSGAISESSDVQKLTKSDY
                     MPEQPIFNSGIRDNREYFIEIVENSNLFNHESEKSKNKVYDNIRLEKVDIIVNEGKKD
                     TIYKAKLAEGLDDGKTNNSNRNQVNYEVSFPKRDIDDIIYIKAKLTYNNGVEYTKDIT
                     EDITMGLQDLEK"
     gene            complement(36709..37200)
                     /locus_tag="MJE33_000700"
     CDS             complement(36709..37200)
                     /locus_tag="MJE33_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY9646669.1"
                     /translation="MADKMAQNITLQFVEEKDLENLENVKLLFTEYSNSLNIDLCFQD
                     FNNELKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEGKICELKRLYVRNQFRGLKI
                     GKILLEEIIEEAKKFGYTHMRLDTLPSMKSAQGLYEKFGFYDIEPYTYNPIEGARYME
                     LKL"
     gene            complement(37261..37701)
                     /locus_tag="MJE33_000701"
     CDS             complement(37261..37701)
                     /locus_tag="MJE33_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY9646670.1"
                     /translation="MKLNVRIADINNWHDLVSLSVDKSQLDYIESNALSIAESKFITA
                     WVPVGIYDENSLIGFAMYGRLEDDRVWLDRFMIDSKYQGKGYGKASLDFLVNHLKNEY
                     NCDELYLSIFEDNKMAIKLYKDFGFEFNGELDYGGEKVMVLKSN"
     gene            37862..38920
                     /locus_tag="MJE33_000702"
     CDS             37862..38920
                     /locus_tag="MJE33_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HBY9646671.1"
                     /translation="MNKINKNFKNIWIILGAVSIFTMVIIFNKNCNVNKETQIEAFTI
                     KNHDKKFVYPMGNIIGVKANTDGVLVLGYEEEDVDYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKSKVEVTFERKNKYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNITENHNKEFNVANKEKQLIEVGRPQDVKIGDAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            39062..39886
                     /gene="spo0A"
                     /locus_tag="MJE33_000703"
     CDS             39062..39886
                     /gene="spo0A"
                     /locus_tag="MJE33_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HBY9646672.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
     gene            40299..41420
                     /locus_tag="MJE33_000704"
     CDS             40299..41420
                     /locus_tag="MJE33_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646673.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSHQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            41438..42313
                     /locus_tag="MJE33_000705"
     CDS             41438..42313
                     /locus_tag="MJE33_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HBY9646674.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFDILKEKNDKLDSDLASVNRDYEEAVNFINKNVDKILAGSLNGKSIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIILKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNFIGDTYLKYDSVVIAGGNDAKDSAKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            42337..43029
                     /locus_tag="MJE33_000706"
     CDS             42337..43029
                     /locus_tag="MJE33_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBY9646675.1"
                     /translation="MDSYISIIIPAYNEESRIKYTLESILDIKQINEIIVVDDGSIDN
                     TAKVVSELENEKIKFFKLDKNRGKGYALNYGIKLAMKNADIIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            43037..43861
                     /locus_tag="MJE33_000707"
     CDS             43037..43861
                     /locus_tag="MJE33_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBY9646676.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPLFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKDLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIIFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYPFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            43866..44933
                     /locus_tag="MJE33_000708"
     CDS             43866..44933
                     /locus_tag="MJE33_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HBY9646677.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGEIQKAYNYPKISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFAASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDTLKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADAVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLDKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            44935..45471
                     /locus_tag="MJE33_000709"
     CDS             44935..45471
                     /locus_tag="MJE33_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBY9646678.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     IKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVYEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            45539..46144
                     /locus_tag="MJE33_000710"
     CDS             45539..46144
                     /locus_tag="MJE33_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HBY9646679.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIVVGSYINKILPGSSNN
                     ILNNINPAVEYYNLNISIKDTVIQNLKSDAIFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILALISLSYFKEMVYEFKKKNR
                     KNMQFLIKRYILNIIIIIALSLGLQLILNTASIGIIKFLAK"
     gene            46156..47070
                     /locus_tag="MJE33_000711"
     CDS             46156..47070
                     /locus_tag="MJE33_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBY9646680.1"
                     /translation="MDIIEGYIDYIKDKKKLSENTVASYFMDIKKYMEYINQKSIKLV
                     DIVENDIISYLIKLEKDNVSIATIARMISSIKSFHDYLFLNHICTNNPAKDIKKPKIK
                     KENINILTEEEIERLLNFPKLTTPKLIRDKAIFEVLYGTGIKVSELVEMNIEDIDLDI
                     DYIYCNSCCKNQRVIPLCDITKLYLEKYLKEARPKMAVEGEKSLFVSSLGQRFTRQGL
                     WKVIKKYSNLANIDKNINPTMLRHSFAIHLLNEGANIAVVSKILGNVNLSSLQVYLNH
                     IDKNVRREIKEKHPRNDVDVELQKAEAK"
     gene            47112..48284
                     /locus_tag="MJE33_000712"
     CDS             47112..48284
                     /locus_tag="MJE33_000712"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBY9646681.1"
                     /translation="MSRVIWIVIDSVGIGALPDAEKFGDSKDVSTLGNIFKEYPDIQI
                     PNMRNLGIGNIDGIDFFESIKEPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EHGFSKEIIEEFEKKTGRKVVGNKPASGTVIIDEYGEHQIKTGDVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMREDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKEDLF"
     gene            48331..49143
                     /locus_tag="MJE33_000713"
     CDS             48331..49143
                     /locus_tag="MJE33_000713"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBY9646682.1"
                     /translation="MFEKIAQSSKFINSKSDIKPKIGLILGSGLGDLANDIEDPVAIK
                     YSEIPNFPVSTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEILIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRFGARFPDMSDAYSS
                     KYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQPLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            49347..50672
                     /locus_tag="MJE33_000714"
     CDS             49347..50672
                     /locus_tag="MJE33_000714"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBY9646683.1"
                     /translation="MRMYDIIKKKRDNLELSKKEINYFIENYSKGDIPDYQASALLMS
                     IYLNKMNKQETVHLTEAMMNSGDMINLSEIDGIKVDKHSTGGVGDKTTIALIPLVASC
                     GAPVAKMSGRGLGHTGGTIDKLESIPGFSTEMEISKFIDSVNKSKIAVCGQSAKVAIA
                     DKKIYALRDVTATVDNISLISSSVMSKKLASGADAIVLDVKTGDGAFMKTLEESFELA
                     KSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVELCEVLG
                     AYMLVLSKVAHDFEDGVEKIRESIKSGTAIQKLKEFIENQGGNKDVVDDYSLFKKSKY
                     KYPVKSTKSGYISKIKAEDVGVSAMILGAGRETKDDILDLSAGIILEKKVGDYVNEGE
                     VLAYMYYNDEQKLSLAKGRFIDSYSIVDSKIEKNKLIYGIVTKEEIKKF"
     gene            50885..51964
                     /gene="mltG"
                     /locus_tag="MJE33_000715"
     CDS             50885..51964
                     /gene="mltG"
                     /locus_tag="MJE33_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBY9646684.1"
                     /translation="MNFKENRLKIAVLIIVILIILAGIFVFIQIGPYDKNNKKDVIID
                     VPSGASVGKISDILYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISL
                     LVSGKIYQDGIKVTIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFPYLKE
                     DGITSLEGFLYPETYYFNSKKQSEEDILSEMLKVFDSKYTDKFKKKQKELNMTLQEVM
                     EMASIIEKEAVLDKDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKID
                     SPYNSYKNKGLPPTPISNPGIKSIEAALYPEKTDYLYFVAKIDGGNNYSTNYQDHLKY
                     VKEYKEARDKQSKDTKATNKENTKK"
     gene            52136..52810
                     /locus_tag="MJE33_000716"
     CDS             52136..52810
                     /locus_tag="MJE33_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBY9646685.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVS
                     DLLKVLLKVQKPKRILEVGCAIGYSSIFFATIIGKDADIITVERNEKMIEKAKENIKL
                     AGFDNNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRNYLDYICNCDYLSTSLVPIGDGVALSYKESKRG
                     DVDGIK"
     gene            52797..54044
                     /locus_tag="MJE33_000717"
     CDS             52797..54044
                     /locus_tag="MJE33_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBY9646686.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEEIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEISEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YRDPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEEMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            54116..55780
                     /locus_tag="MJE33_000718"
     CDS             54116..55780
                     /locus_tag="MJE33_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBY9646687.1"
                     /translation="MSKKKTPFLKKVGKRSWCIFTIILIIYSVLIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKSQVLIVEKEKLNNNYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQTKNIDKSMKKELEKNGIMVEEKTMRYAKDGLLS
                     HTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKTVD
                     NKDKDRHLKTTIDYNIQKKLEQILNKEENPTAAIISEASTGEILAMCSRPNFDQNDIS
                     KSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNTNE
                     ILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGLDE
                     EKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIIANNGTFKPLYLYKSLVDNNMNT
                     IKTYKSSKKEELISPYVCTQVKEYMKSVSRIGTAKDLKDIEGGCGVKTGTAQSSLNKK
                     AIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIK"
     gene            complement(55877..56161)
                     /locus_tag="MJE33_000719"
     CDS             complement(55877..56161)
                     /locus_tag="MJE33_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBY9646688.1"
                     /translation="MCNEISLLDILGTEANYVLDEVELKVQVGKLYEQLNKILTPRER
                     EIVQLRYGLTPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
     gene            complement(56184..57701)
                     /locus_tag="MJE33_000720"
     CDS             complement(56184..57701)
                     /locus_tag="MJE33_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBY9646689.1"
                     /translation="MSVAIYLRKSRADEEAEKQGEFETLSRHKSTLLKLAKEQNLDVI
                     EIKEELVSGESIIHRPKMLELLKEVEENKYDAVLVMDLDRLGRGDMKDQGIILETFKE
                     SKTKIITPRKTYDLTDEFDEEYSEFEAFMARKELKLISRRMQRGRIKSVEEGNFIGTS
                     APFGYDAVTTGRKERILVPNKDADVVRTIFDLYINEDMGCSKISKYLNNLGIKTATGA
                     NWYNSAITNIIKNKVYCGYIQWQKKDYKKSKNPNKIKTVKLRPKDEWIEAKGKHEPLI
                     SEITWKKAQNILKKNGHVSYGNQIKNPLAGIVICKNCGRPLVYRPYADHDYIICYHPG
                     CNKSSRFEFIEAAILKSLEDTVKKYQLKASDIDLDKNNKDSNIEFQKRVLKGLETELK
                     ELGKQKNKLYDLLERGIYDEDTFIERSNNISSRTEEIKDSIKTVKNRLSTVKKDNSKI
                     IEDIKTVLSLYHDSDSLGKNKLLKSVIDKAVYYKSKEQKLDSFELMVHLKLHEDQ"
     gene            complement(57826..58647)
                     /locus_tag="MJE33_000721"
     CDS             complement(57826..58647)
                     /locus_tag="MJE33_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBY9646690.1"
                     /translation="MIISKKITAILVSFLIMISMVGCAPKESATEVAKKYLDSIKIKD
                     YEVAYNLLSKDSKESVSLEKFEEYQDTLFKLQTLKDYKLGEENKVEKYKYSNKEYTNL
                     VKIKETDTIKYITENKEDTANFDRYFIIEDNEYKLLWHKDFKRALSSNYVKIARSETE
                     KDEDKDYNKAAIDIKKAIEIDDKNPLAYYTQSYIYIELQRYDEALKSIDKYLEYLDKN
                     NEAYKIDASDAYNLRGVIFMSKSEFNKARESYNKALELNPDNEYAKTNLNDIKGL"
     gene            58838..60265
                     /locus_tag="MJE33_000722"
     CDS             58838..60265
                     /locus_tag="MJE33_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp26"
                     /protein_id="HBY9646691.1"
                     /translation="MKIKKLLTIGLSLSIFIASCPISANALDKIESIKGADKYETAGI
                     IADKQNYTTAILINADSTMSDGLSASGLAGAINAPILLTKKNNIPNATLKRLEKAKKV
                     YIIGGENSIDKYTETVLKGKGIEIKRLQGSDRIKTSYNVAKEINSINKVNKVILTNAF
                     KGEPDAMSVAPVAVRDKAAIVLTDGKSVPFNTTGVESYVIGGTSSMNDKIVKDTNSTR
                     LGGTDRYDTNKKIINKFYSGVKEFYIASGTDLVYALVGSTAAKNNAIVLVDNDSNKSV
                     LKSTTKLTAIGNLSDSILQQCLNVTKNIGDSNTGREYTVEDAANAVKKLLGVKKFYYK
                     PNYSEDEDLIFLLDKNFDFSGKEYYVFAYIKQGAEGDVRYCVAKNDMSKVYTYDVNGN
                     MKVCNPDDYKNKGSITEEQAKQIALKECAKRHKVSINNLVVDMIDFRDDIYSGNVYAV
                     KINSKNSNGTFGWFFIDIETGKVYN"
     repeat_region   61221..61579
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=61221..61249
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaag"
     gene            complement(61866..62090)
                     /locus_tag="MJE33_000723"
     CDS             complement(61866..62090)
                     /locus_tag="MJE33_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY9646692.1"
                     /translation="MDVNQRIKYIRENMNSSQKELLEKTNINTSVMNRIESGERPTRD
                     DGLIIFAKIFGLYTDYILGLSDTEKLNIGN"
     gene            62801..62956
                     /locus_tag="MJE33_000724"
     CDS             62801..62956
                     /locus_tag="MJE33_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HBY9646693.1"
                     /translation="MALDVKIEVKIIKDVVILNITHWKKETSLLTVKIARSQGDLSYV
                     KYFANES"
     gene            63202..63453
                     /locus_tag="MJE33_000725"
     CDS             63202..63453
                     /locus_tag="MJE33_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646694.1"
                     /translation="MSSYFITRGDVMKILQQFKPYKYDKEKNIEYDEKIIANQNTEVY
                     IVYPRISDEENRRRWEEFNEVAKEVAINIAMKKVETVES"
     gene            complement(64663..65046)
                     /locus_tag="MJE33_000726"
     CDS             complement(64663..65046)
                     /locus_tag="MJE33_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646695.1"
                     /translation="MRKKFFIGILIVLIISVIAILNIKNDKNVFIGESENWKAKFVTT
                     EGHSKLEIKYKNSKDVVEQKFNYSYQFIDGEDAVVVVKNEILEAGQEKAIKIQRPGLY
                     SNRESSYKLIISWNGNEETINLKKK"
     gene            65173..65517
                     /locus_tag="MJE33_000727"
     CDS             65173..65517
                     /locus_tag="MJE33_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646696.1"
                     /translation="MNYRLKKSVTTILSLLIMTILLPTNASASKVEDSSMKVMYSEKQ
                     VTDMDKLYDMAKNGITDVKSSNEKGIIKNARTGESIYVDSISTTQLLEVKQSKNLTSK
                     IYSKTVFLILIQ"
     gene            65700..65915
                     /locus_tag="MJE33_000728"
     CDS             65700..65915
                     /locus_tag="MJE33_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646697.1"
                     /translation="MHINQTGFISNELGIKQQQKKEPTSNSFSYNAPSSWKPVGRDSV
                     QYNCGFELIATLVRGGSSWAFNFIVQP"
     gene            complement(66900..67217)
                     /locus_tag="MJE33_000729"
     CDS             complement(66900..67217)
                     /locus_tag="MJE33_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646698.1"
                     /translation="MNFRNIYLILMGLGIIILTTIISLIEIKIGFCNEKYFNKLESKY
                     GNIDRKRTIKLEVLYRYVTGFEYIAIGLFTKRLDITIIAIILVATITVILYYLVRKRY
                     ITI"
     gene            complement(67527..67854)
                     /locus_tag="MJE33_000730"
                     /pseudo
     CDS             complement(67527..67854)
                     /locus_tag="MJE33_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889008.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        27032 a         7095 c        11899 g        21828 t
ORIGIN      
        1 tctgaagcta aattgtaaaa tttttaggat ggcaatattt acttaaattt agaaagtaac
       61 ataatattta agttgatgtt gtcatcctaa aagcatgtgt acataaataa agtaagacta
      121 ttgtatctta tataataaag agttaataaa atgattttta taaatatagt tattttaaaa
      181 aactacttta catatattgg atgagtataa aaatccatag ggccttttac taaatcttta
      241 ttgtttttat aatatattga attatatata tttgtagctt ttatatcata acttatgagt
      301 gttttaaaaa tcgtatcatc taatgaaaat gatatatttg aaaacttgtt tattattttt
      361 atttcagatt tattttttat ctcttttaga atttctctac ctttatcatt aaaagcaagt
      421 attcttatat aaggtatagt atctatgttt tttataagtt gcacgtcgct ttttttaata
      481 cctaacaata tattgtttag agttcgtttg attttggtta aagtgtatcg cttggattta
      541 attgctaatt gtagttcatg tagaaattca gatgtaaaga tatctttata gattctgttt
      601 tctatgcctt catttacttc aaaatactct tttaaagtgt ccatatctct aagtataatt
      661 gtttttaaaa tatcaaagaa gaagtcatca aacataggta aaaaaccatt atctattttt
      721 tcatttagga tgttataagt tttttctgga ataacatttt ttaaatgaaa aaggtctgta
      781 ttttctttaa gtgaagttct tatagcagta gcagaacaaa tatcactctc aatttcttcg
      841 gaattatatt ctgattggac cctggagata gtaaatggtt ttatactact gtttaaatgg
      901 atgaggtttt ttatgtattc tatacccagt atgttgtttg atgaatttaa tgtttttgat
      961 aattgttcct ttgaataatt ttgtataaaa ttattttttt tgtcttgatg tatataatca
     1021 aaaagtgcat tagctctagc tgttggaaat aacagaccat catctaaata tttttttaaa
     1081 agtaacttaa attcatttgg ttcatttgct aaaatttcag ctagattata aagaatttct
     1141 gtatttcctt cttcactacc aaaacatata gaatttatgc agtttagtga atttaaaatt
     1201 gttatagcac catgagaaaa aatttcagca ctttgacaag caaataaagt tggtaattct
     1261 ataactaagt ctacaccatt ttcgacagca atttttgctc tagtgtattt atcaaataaa
     1321 gctggttcgc ctctttgtag aaaatttcca ctcattattg ctacagtatg tgttgcattt
     1381 gttttttcta tagatttaga taaatgatat atgtgtccat tatgaaatgg gttatattca
     1441 actattaatc caagtatgtt cataaaatac tccttttatc tttaattatt ttatatatat
     1501 cacaatttat tataatatgt ataaaattta aatttttaat tataaaatta aaaaacgtaa
     1561 aaaaatttat aaaaatgtag aaatgttacc tacataaaaa actggttaaa tgttattaaa
     1621 ataactttat tagctcttta tattatatta ttataacaaa tgggaaagaa taatctacat
     1681 taagttaact tgttaatata ttattagtga aatacacaat ttaccatact agaaatatga
     1741 tataatataa aaaaaaagta tatactttta tattaaaagg tgatatatta tttatgaaaa
     1801 tcgtttaatg tatacaataa tatatctata acaaaataat aaaagataga gttttattta
     1861 gacatttatt acaaatcaaa atttattaca tattatttat aaaaaaatct aaggaggaaa
     1921 taataatgaa aatattagta ttaaactgtg ggagttcttc attaaaatat caattaatag
     1981 atatgaataa tgaagaagtt ttatgtatag gattggttga aagaatagga atagaaggtt
     2041 ctatattaaa acatgaaaaa gcaggtagag atgataaata cgttgttgaa caaccaatga
     2101 aggaccacaa agatgcaata gcattagttt tagaagctgt tgctcatcca gaatttggtg
     2161 cagttaaaga aatgaaagaa atagatgcag taggacatag ggttgtacat gctggagaaa
     2221 aatttgcaac ttcagtagta ataactccag aagtagaaga agctttaaaa gaatgtatag
     2281 atttagctcc acttcataat ccagcaaaca taatgggaat agatgcttgt aaagctatac
     2341 ttccagatgt accaatggta ggtgtatttg atactgcttt ccatcaaaca atgcctaaat
     2401 cttcatactt atatggttta cctcatgagc tatatactaa atatggggta agaagatatg
     2461 gattccacgg aacttctcat aattatgtat ctcaaagagc agcagagata ttaggaaaag
     2521 atataaaaga tttaaaaata gtaacttgtc acttaggaaa tggtgcttct atagctgctg
     2581 ttgatggtgg aaaatgtgta gatacatcta tgggattcac tccattagaa ggattaataa
     2641 tgggaactag atgtggagat atagacccag ctatattacc attcttaatg agaaaagaag
     2701 gtttagatgc tgatggattg gataaattaa tgaataaaga atctggagta tatggaatga
     2761 ctggaatttc tagtgacttc agagatatag aagatgcagc taaaaatgga gacgaaagag
     2821 ctcaagctac attagaagcg tatgttaaaa aagtacaaaa atatataggc gcttatgctg
     2881 ctgaaatgaa tggattggat gttgtagtat ttactgcagg cgttggtgaa aatggaaagg
     2941 ctataagagc agatattgct tctaacatgg agtttttagg aatgaaatta gataaagaag
     3001 ctaatgatgt tagaggaaaa gaaacagtaa tatctactgc tgattctaaa gtaaaaatgc
     3061 ttttaatacc aactaatgaa gaattaatga tagctagaga tacattaaga ttggtaaaat
     3121 aattaaatta aacctatgcc tatcaagtaa aaatacttga caaacaaatg ctagatttgt
     3181 ataataaatt tggtgatatg ttgaggtgaa cttaatgaaa gttagcatag aaaaaataaa
     3241 tagaaaagaa actgacaaaa tagacttgaa tttttgtgaa aaaattgata ctattagtta
     3301 ttgtgatgag atttacaaat tagtatcacc tgtaaattta aaagggaaag tgtcaaagac
     3361 taataaaggt ttgtatctag atatagatgt caattttaca attgttgaca attgttcaag
     3421 atgtcttaaa gaagtagaaa taccactaga gtattctata caaggttttt tagtaaaaga
     3481 agaagattat gatgaagatg agtttgagga atttgatcct tttatattcg atggtgaaga
     3541 aatcgacctt attgatataa tagaacagac attagatttt aatgtacctc ataaagttct
     3601 ttgtagtgaa aactgtaaag gtctttgtca agtatgcggg gcaaacttaa atgaagaaga
     3661 gtgttcttgc agtgaaatta caaacgatga ggaatacata gatcctcgtt ttgctaaatt
     3721 aaaagattta tttaattaaa ttttgactaa ggaggtggcg taaatggcag taccaaagcg
     3781 taaaacgtct aaatcaaaca caaaaatgag aagagcagct aactcaaaga tggaagcaac
     3841 aggatttgta agttgcccac aatgtcatga gccaaaatta ccacatagag tgtgtccaga
     3901 ctgtggatat tataaaggta aagaagttgt gtctaagtaa tagcttctaa aaagcatgta
     3961 gagtatactc tacatgtttt ttttgtacat ataaaaaaat aagataattt actagagaaa
     4021 ataagtggat tttatgcata ttaaaaaata gaaaaataat aatataagta ttgaaaattt
     4081 ttgtgtgtta ctatatacta ctattagtag ctggtactaa ttacagctat aaaatttgga
     4141 gggaacatga aaaagaaaag taaagcccaa agacaaaaag agcttataga tatgctaaag
     4201 acagatcctt tttatactga tgaagaatta tcaagtctgt ttgatgtaag tatacagacc
     4261 ataagattag atagaatgag tctaaacata ccagagctaa gagaaagagt caaatcaata
     4321 gcagaaactc agagttcaaa agtaaagact ttaggagtaa aggaaattac aggagaaatc
     4381 attgatttat ctgtagggcg attaggtata tctatgttag aagttacaca ggacatgatt
     4441 tactctaaga ctaatacttt aaaagatacg tatatttttt ctctagcaga ttcattggca
     4501 atggctataa tagatgcacc taaggttatt atgagagttg caaatgtaaa aagttttaag
     4561 ttgatagaac aacaagatag gcttatagca aaagcagaag tgtatagaaa tatagataaa
     4621 aagcattatg taaaagttgt tatcaacaat aaagcccaag agcaaatatt tagaggaaag
     4681 tttatctttg aagaattaga ttaggaggga aattatgaaa atagtaattg atggaatggg
     4741 tggagataat gcacctaaat ctaatgtaga aggcgcagta aatgctataa aagaatatca
     4801 ggttgattta ataatcacag gagataaaga tttacttgaa aaggaatttt caaattatga
     4861 atttgataga aataaattag agatagttca tactactgag attatagaaa acgaagataa
     4921 accagtaaag gctataagaa gtaaaaaaga ttcatctatg gtagtagcat taaatcttgt
     4981 aaaagaagga aaggcagatg ctataatatc agctggaaat acaggagcct tacttgctgg
     5041 tggattattt gttgttggta gaataaaagg aatcgataga ccttgcttat gttcagcaat
     5101 accaaatgta aagcgaggaa tgacacttat agcagactgt ggcgcaaatg cagattgtaa
     5161 acctaagaat ctagttgaat ttgcagctat gagtaatatt tattcaagaa aagttttagg
     5221 tcttgaaaat cctaaggtag cattagcaaa tgttggacta gaagaaggca aaggcaatga
     5281 tttagtcaaa aggtcatatg aagaaattaa aaaattagat ttaaatttta tagggaatgt
     5341 agaagctaga gaagttataa atgcatacac agatataatc atatgtgatg gatttacagg
     5401 taatatatta cttaaatctg ctgaaggtgt agcactttca gttatgagtc ttataaagga
     5461 aacttttatg gccagtacaa aaagtaaaat aggagcatta cttataaagg atgatttaag
     5521 aaaattaaaa agttttatag attattctga gtatggggga gctccacttt taggtctaaa
     5581 tggaggagtt ataaaagctc atggtagttc tgatgctaaa gctataaaaa atgctataaa
     5641 tcaaggaata aaattttcaa agggaaaagt cgttgaagat attaaccaat ttataagcaa
     5701 atacaatgaa gaaaataaaa ataacgaaga tgaataaact cgttttggag gtttaatata
     5761 taatgaatac aaaggctgga atattaggag taggaagtta tctacctgag caatcatatg
     5821 ataattttca cttcgaaaaa ataatggata catcagatga gtggataagt actagaactg
     5881 gtattaagga aagaagattt gccaaagagt ctgaagcaac ttctgactta gcatctaaag
     5941 ctgccttaaa agctatagaa tgtgcaaagt taaacgtaga ggatatagag ctgattatat
     6001 tagctacaat tactcctgat atgtctttgc cttcaactgc atgtatagta caagatgcaa
     6061 taggagcagt gaatgctact gcatttgata tatcagcagc ttgttctgga tttgtatatg
     6121 gagtaactat tgcaaagcaa tttgtggaaa caggttgcta taaaaatgtg cttgtaatag
     6181 gagcagaaac ttgttctaag tttttaaatt atgatgatag aacaacagca gtattgtttg
     6241 gagatggagc tggagcagct gttattggcc ctgtaaatga gggtggaata ctgtctacac
     6301 atatgggctc tgatggtaaa ggaaaagatt gtttaaaagt acctgctgga ggttcaagac
     6361 ttaaggcaag taaagagact gtagaagcta atctacatac aatagaaatg gcaggtagtg
     6421 atgtgtttaa atttgcagtt agaaagatgg cagaaacttc tttaagagct cttgaaaaag
     6481 caaatctaaa tacaactgat atagactact tagtgccaca tcaagctaat ataagaatca
     6541 tacaagcttc ttctaagaga ttagagcttg atatgaaaaa agtttatgta aatatagaca
     6601 aatatggaaa tatgtctgct gcatctatac cagtagcatt agatgaagct tatagagaag
     6661 gtaaaattaa aaagggtgat aatgtagttt tagttggctt tggtggaggt ctaacttggg
     6721 gggcttcagt tgtcaagtgg actttatagt ataaaatatc taaaatgatt taattaacaa
     6781 aattaaactt atacacaaaa taaagttgat taataataaa aagtttactt ttatgatttc
     6841 aagtgaagga gaattgtatg aataaaattt gcaaaatatt aaatataaaa tacccagtta
     6901 tccaaggggg aatggcatgg gtagctactg catcattagc aagtgctgta tctaatgcag
     6961 gaggacttgg cataatagca gcaggaaacg caccaaaaga agctataaag aaagaaattg
     7021 ttgagtgtaa aaaattaaca gataaacctt ttggagtaaa tgtaatgctt atgtcgccat
     7081 ttgttgatga tataattgat ttgattatag aagaaaaagt tcaagttatt actactggtg
     7141 ctggaaatcc tgcaaagtat atggatagat taaaggaagc tggaacaaag gttattcctg
     7201 tagtacctac aatagctttg gcacaaagaa tggaaaagct aggagctaca gcagtaatag
     7261 cagaaggtac tgaaggtgga ggacatatag gagaacttac tactatggtc ttagttccac
     7321 aagttgctga tgctgtaaac atacctgtaa tagctgctgg aggaattgta gatggtagag
     7381 gaattgcagc atcatttgca ttaggtgcca gtgcagttca agtaggaact agatttattt
     7441 gtagtgaaga gtgttctgtc cattcaaact ataaaaactt agtactaaaa gcaaaagata
     7501 gagatgcaat tgtaacagga agaagtactg gtcatccagt aagaacatta aaaaataaac
     7561 tatcaaaaga atttttaaag atggaacaaa atggagctac tcctgaagaa ctggataaaa
     7621 aaggtacagg agctttaaga tttgcaacag tagatggaga catagaaaaa ggttcattta
     7681 tggcaggtca aagtgctgct atggtaaaag aaataacacc ttgtaaggaa attatagagg
     7741 ctatggtaaa tcaagcaaga gagattatgc cagcaataga actgtaaatc aggaggagca
     7801 agatgggaaa agttgcacta gtattccctg gtcaaggagc tcaatatgta ggtatggcca
     7861 aggatttata tgaaaataat actgttgcta aaagtgtaat agatgaggct agtgatgcct
     7921 taaatatgga tttaaaaaac cttatgttta atggaaatga agaagaactt tctaaaactg
     7981 aaaacactca acctgctatg gttacacata gtgtagcagt attaaaagcg gtacaagctc
     8041 aaatagattt aaaatataat gcatgcttag gattatcctt aggagagtat agtgcgttgg
     8101 ttgcagcaga tgcaattgac tttaaagatg ctgtatgttt agttaaaaaa agaggtaagt
     8161 ttatgcagga aacagttcca gcaggtgttg gagctatggc agcaatttta ggtttagata
     8221 gaaatgtact tgaaaatgtg gttaaagata tacaaggagg tattgtagaa gtagcaaatt
     8281 acaattctcc tggacaaata gttatatctg gtgaaaatga aaagatagaa gaagcaatgg
     8341 taaaatgcaa agaagcagga gcaaaaaggg cagttaaatt aaatgtaagt ggaccttttc
     8401 actcatcaat gttaaaagaa gctggtgtaa aattggccag tgaattagaa aaagttaaca
     8461 taactaaacc taaagttgat gtggtggcaa atgtaaatgc agattactat aaaaacgaag
     8521 gtaaagacct gcttataaaa caagtaagtt catctgttct ttgggaagat tctatagaaa
     8581 gacttttaaa tgatggatat gatactttta tagaaatggg acctggaaaa actttaaaag
     8641 catttattaa gaagatagct tccaataaaa aagcaaatgt caatatttat aatatagatg
     8701 gtatagattc tcttaatgaa tttgtacaaa attatagaaa tggagagata taatgataaa
     8761 tcttacagga caagtagcag ttgttactgg tggctctagg ggtataggaa aagaaatagc
     8821 aaaaaaacta gcatcttttg gagctgatgt agtaatcaat tatacttcta aagaagatga
     8881 agcactaaaa actaaaaatg aaatagaaag tatgggggta aagtgtacct ctataaaatg
     8941 tgatgtatct aaatttgatg aagtgaatca aatgatagat tctgttgtaa gcgaatttgg
     9001 aaaaattgat atattggtta ataatgcagg cataactaaa gatggtctgc ttatgagaat
     9061 gaaagaagaa gattttgata gagttataga tataaactta aaaggtgtct ttaattgtac
     9121 aaaagcagtt actaaaccta tgatgaaaaa gaagtatgga agaataataa atatgacttc
     9181 agtagttgga attatgggta atgcagggca aactaattat tgtgcatcaa aagcaggtgt
     9241 aattggattt acaaaagctt ctgcaagaga gttagcatca agaaacataa atataaatgc
     9301 agtagcacct ggatttatag aaacagatat gactaaagta ctaagtgatg atgtaaaaga
     9361 atcaacacta gcaaacatac caaagaaatc ttatggtaaa ccagaagatg tagccaatgc
     9421 cgtagcattt ttagttagtg acatgtcaag ttatataaca ggacaagtaa taaatgtaga
     9481 tggtggaatg gtaatgcaat aataatttaa aaagtagaga aatctattaa aataaaaaat
     9541 tttaggaggt acacaatgtt taataaagtt gtagaaataa taatagagca attaggagta
     9601 gaagataaag aaataactat ggaaacatca ttaatgaagg atttagaagc agattcatta
     9661 gatgcagtag aaataataat ggcacttgaa gatgaatttg gtatagaaat accagataca
     9721 gaagcagaaa acttcaaatc tataggagat atagttaagt atatagaagc taataaatag
     9781 tcgagcttta aaaaagtgga ggtatccgat gaataaaaga gtagtaataa caggacttgg
     9841 atgtgttacg ccgttgggaa caggaaaaga agagttctgg agtaatataa aatctggagt
     9901 atctggtata gataaaataa ctaattttga tgctagcaca tatcaaactc agatagcagg
     9961 agaagttaag aattttcatc cagaagaata tataagtaaa aaagaattga agagattaga
    10021 taaatttgct caatttgcaa tagtatctgc taaattagca gttgaagatg caaatcttga
    10081 tttagataaa gttgatagag aacgttttgg tgttataata ggttctggaa taggtggagt
    10141 tgaagctata gaaactcaac ataaaattct tttggaaaaa ggaaataaaa gagtaagttc
    10201 actttttgta cctatgatga taggaaatat ggcagctggt caagtttcaa tatttttagg
    10261 agcaaaaggg cctaacacta atgtatgtac agcttgtgca tcaggaactc attcaatagg
    10321 tgatgctttt aaggtaattc aaagaggtga tgcagacatc atggtagcag ggggctctga
    10381 agctgctgta acaggtcttg cttttgctgg attctgtaac atgaaggcta tgtcaactag
    10441 aaatgatgac cctaaaactg catcaagacc atttgataaa gacagagatg gttttgtaat
    10501 gggagaagga gcaggtattg ttatcttaga agacttagaa catgctttag caagaggcgc
    10561 taaaatatat gcagaagtag ttggatatgg tttaactgct gatgcatacc atatgacaac
    10621 tccagctgaa aatggagagg gagcagctag gtctatgaat atggctttga aagatggaaa
    10681 tgttccatta gaagaagtag attatataaa tgctcatgga acttcaactt attacaatga
    10741 tttatatgaa actatggcta taaaaactgt atttggagaa aaggcatatg acttatgtgt
    10801 gtcatcaaca aaatctatga ctggtcactt attaggagca tcaggtgcta tagaggctgt
    10861 tgtttgtgct atgagtatag aagatagttt tgtgcctcca actataaaca tacaagaagt
    10921 tggtgaagat ttggatttag attatgttcc aaatcaaggt aaagaaaaaa atattagata
    10981 tgcactatca aattctttag gatttggtgg gcataatgct acaatagtac taaaaaaata
    11041 tgtataaata aatattaaat ataaaactaa aaaacaagca cttgttttaa ctgtataaat
    11101 ttatattagc ttaaatttaa agtctcctaa attattttag gagacttttt tattaagatt
    11161 taagtctagc atatgttgta aatcattttg atatattgta ttattaatta ttaagaggtt
    11221 atttatatat gcaagttttc attctacaat atacaaaagg aggactaaaa ttttgaattt
    11281 tagactacca aagaaaaggt tacccaaaaa gataattttt atatgtataa tacttattag
    11341 tattattttg tttataaagt atgaccaaaa tggattgaaa gaaagagaaa ttataaataa
    11401 gggaaaatat atgtctgaaa gccttgtggc agataaggag tatgatgaag caaaaaaagt
    11461 ggttaagact tctttcgaat ctattccaga aaaaaaatac aataaagtat ataatgaagt
    11521 ttgggatatg ttaaagacta tttcatatgt tccagatgga acgaagatag tcattgattc
    11581 tttagaaaag atgagagaat cagatgtgaa attatctgat gaatgtagat ttaatataga
    11641 aaaaagatta gcatcagtgt atataatgga taataattat tctaaagcag tagatttaac
    11701 tgtaaagtct ataaagttgg ctgagaaatt ggggaataat tatgaaaaag caagactaga
    11761 tgttgatttg gcgagcatat ttttaaaaat aggtagctat gaaacaggag agaaattaat
    11821 aaaggattct tttaaaatag atattgatga aggtgaaaaa aatggaatgg ttagattata
    11881 tgctattata aatctagcag aaatatatgt agaagaggga gaatatgata aagctattga
    11941 aataagtagc aggataaaag attataaaaa gtttgtaaat acggataatt acaatgattt
    12001 tgatataatg gcctctataa tgcagtcaaa tgcgtatgtg ggcaaaaata atatacaaaa
    12061 ggcaaagact tttttagaaa aagctgatta tcttataaaa tcagatagaa cagtttatat
    12121 attagataaa gatatgtact attatatggc aatgggaaac ttagaatata aaagcgaaaa
    12181 ttataattct gcaatagata attataaaaa aagtttagag atatctacta aaagaaagtt
    12241 aacccaggag aaaataaaaa atttaaataa gatattaaat atatatgaaa aacaaggaaa
    12301 tatagaatct ttaaatgagt atcaaaagat gttaataaat gaatataaag agaacaaaag
    12361 tataagagat tcagagacta gtttctatat aattgataaa gtatcaaacg aaagtaagct
    12421 atttgaaaaa actaaaaaag aaataaaata ttataaaata ttatttacag taattttaat
    12481 tgctatatta gggtctactt ttttatacaa ccgattgaag tattttaaag cacaaaattt
    12541 acatgatggt cttacaaatg tttataatag aaaaagtttt gatattatgt atgaaaagta
    12601 cagagagaaa gatgataatt ttgcacttgt gatgatagat attgataatt tcaagctcat
    12661 aaatgataat tatggacata aatttggtga cactgttata aagggaataa caaataccat
    12721 ttgtgttatg ttagaacaag atgataaagt ctttaggtac ggaggagaag aattttctgt
    12781 attaataaga aataaatctg gtgaggaagt agcagatata attgacagta taagggttgc
    12841 aattgcaaat aaaaaatgga atgatgatgt gactgtgact ataagtgcag gtgtagctca
    12901 tatttcagat agcaaagatg tactagaaga agctgataaa aatttatata aagcaaaaca
    12961 acttggtaga aataaagtag tatataagta attatatact atataaataa gaggtataaa
    13021 tatgaaacaa tcaatagcta tagtaactga taaaataact aaactatcat ctttcttaga
    13081 agaaaatata cgtttggttt taggtaatta tattgatata aaccactatt atctggaagt
    13141 tttaaaagag aatgataaaa tacaaggtga ttttgtacta gtaatgaatg atgatagatt
    13201 aaacagtatg agaaagcatt tagtacaaga tagtaagatt atagtgataa ggagaacact
    13261 taaagaaaat gaaatatatg gattattttc aataccaaat ggaactgaag tattggttgt
    13321 aaatgataca aaagaaacta ccttagaaac gataagctta ttttacaaaa taggagtaaa
    13381 aaatttaaag tttactccat atattgaagg aaaaatttat aaaaatatag acatagctat
    13441 aacgcctgga gtaaagggaa aagtaccaag ttatataaat aaagtaatcg accttggaaa
    13501 tagatatatt gatatatcta cctttataga aataataaat ggtcttagaa tagatgttaa
    13561 agaaatacgt aaaaatttaa tggcatattc tgagagttta ataagtttag ataatggagt
    13621 taaagataat tataggcaac tgtttttaaa aatagaagaa caagatgtta tattgaatct
    13681 atcaaaagat ggaataatat ttacttcagc agatggtata ataaacacat ttaataaaga
    13741 agctttaaaa attttaggta taaatgaaaa tccacaaggc aaaaacatag aagatatcat
    13801 ttctgaagat ttaaatattt tacttgaaga caaagaaata attgatgaag tggtatttac
    13861 aaataaaaaa tatataaatg taaataaaaa aagtattttt tctatgggga ataaagctgg
    13921 gatatattat aacttacaag aaataacgta tataaaaaaa ttagaacaaa atcttactaa
    13981 taaattaaga gaacaaggtc agatagcaag atatacattt aaagatataa aaactaaaaa
    14041 taaaaatatg gtaaagtgtg tagaattggc taaaaaaata tctaaatcag acctttctgt
    14101 attaataata ggagaaagtg gtactggtaa agaattatta tcacaatcaa ttcataatgc
    14161 ttccaataga agtaatcaac cgtttatagc agttaattgt gcagcagttc cagatagtct
    14221 attagaaagt caactgtttg gatatgaaaa aggctctttt acaggagctt taagagaagg
    14281 aaaaaagggt ctatttgaac ttgctaataa tggaactata tttctggatg aaattggaga
    14341 tatgcctcca ttgctacaga caaagttact aagggtatta caagaaaagc aggttatgcc
    14401 tataggttcc cataatgtta taaacataga tgttagggtc atagcagcaa caaacaaaaa
    14461 cttactaaag atgattagag aaggaaaatt tagagaggac ctgtattata gactaaatgt
    14521 attgccaata gatgtaccat cattgaggaa tagaaaagaa gatataataa cacttatgaa
    14581 tttctttttg aaaaacaata taagactttc gccagaagtt ataactattt tagaaaaata
    14641 taattggcca ggaaatataa gagagttaca aaatgttgct tcttatgtaa gtctaatgtg
    14701 tgatagtatg gtttttagag atgacttacc accatatata agggtagaat gtgctaacaa
    14761 ttatgaagat ggatttggtt atacaagaaa agaatttgag gaaatactga ggattctata
    14821 tgaaagaaga gagtcatcct ctggaattgg aagagggttt ataatcgaaa agcttataca
    14881 ggaaggtatt gagatttctg aaagcaaagt tagaaaggtg ctatcttatt taagtgaatg
    14941 tggatacata tcatctaaat ctggtagagg aggaagtcaa ataacctcag aggggataaa
    15001 actttataat aatataaatt aagctaagta tttgatattg aaatcacata ttttgaattt
    15061 agatatttaa ataataataa acttttatat ttatagagat taatttatat tatgtcagaa
    15121 agaatattgt aatttataat taaatattaa taggttttta aaataggatt agataggtaa
    15181 taggttaagt tagaataacc cattacctat ttttatttgt aagaaaagtg tacatagaat
    15241 tagaagtaaa aaatacaact aataaaacta ttgaaaatac tagataaata ataaataaaa
    15301 ataattatag attttggcat acatattgct aattatagag ataagacgac aaaataattg
    15361 ggaggtttaa tgatgaaata caaaacaaca gggacttgtg ctacagaaat tgaatttgaa
    15421 gtaaaagaaa ataaagttac aaatgtaaac tttataggtg gatgtgatgg aaatttaaaa
    15481 gggctaaaag tattagttga aggcatgaat atagaagatg taatacaaaa gttaaaagga
    15541 atagagtgta aaacaaagcc aacatcttgc ccagaccaac tgtctctagc attggaaaat
    15601 tatatgaata taaagtaata cataaaacaa tcgattagtt aggagtttaa ttatgaaacc
    15661 aataataggg attttaggaa atttaataat aatggaaaat ggtatgtttc caggtcttga
    15721 aaggtcatat gtaaataatg attatataaa tgcagtgcta aaaggtggcg gtagtccagt
    15781 aataattcct gtaaatacag ataaagaagt tataaaaaaa cagattgaaa tggttgatgg
    15841 agtcttaata tctgggggat gggatatcaa tcctcaactg tatggagaag aaacaagaga
    15901 agaaacgaca tttatatatc cagaagtgga tgaatttgat ttaattgcaa taagtatagc
    15961 attagaactt aaaaagccta tattaggtgt atgtagggga ctacaaattt taaatgtaag
    16021 tttaggagga actttatatc aagataataa tcttatagaa ggaagttata taaaacatac
    16081 acaatcttct aaaagacatg tagctactca taaagttgat gttaaagaag gaagtatttt
    16141 agagggaatt ttaggaaaac aattacttac aaatagttat catcatcaaa gtgtcaatca
    16201 gcttggaaaa ggattaaaag ccatagcata ctcaaaagat ggtataatag aagctattga
    16261 gaaagaggat gaaaattttg tagtaggagt acagtggcat ccagaaatga tggtagatta
    16321 ctgtgataaa atggaaaaac tatttaaata ttttataaat atttgttcga atttataata
    16381 taccttgggg ggattgaaca tgaacgaaaa gaacaaaatg ggattaataa gtattatact
    16441 tttaggtatt aatgcagtag taggagctgg agtttttcta ttaccaggag atgcaatgaa
    16501 atcatttggt gtagcaagta tatttgtata tatatttgat atgttgttag ttttatctat
    16561 ggcattctgt tttgctgagg ttgcaggtaa attcaataaa aatggtgctg cctatgtata
    16621 tacaaaagaa gcttttggag atttttgtgg ttttgaagta ggtcttatga aatgggtaat
    16681 aggatgtatt tcttggggag ctttaatagt aggttttcct acatcattat cagcagtatg
    16741 ggctccagca ggtgaaccac atatacaaaa aattataatt gtagctatga tagttgggct
    16801 tacaattata aacttattag gtgtatctct atcaaaaatt gtacaaaatg ttataactgt
    16861 aggtaaacta attccattaa tactatttat aggaataggt atatttttta taaaaggagt
    16921 taattttact tcttcaacaa tggtacctcc aggagctgga gcaacagagt ttggagcagc
    16981 agcactttta atgttttatt catttacagg atttgaatct atagcagttg cagcagaaga
    17041 tatggaaaat ccacaaaaaa atataccaat agcaattata agtgtaatag taattgcatc
    17101 aataatatat atattaaatc aagttgtatg tgttggtata ctaggagatt cgttatctag
    17161 tacatctaca ccagtggcag atgctgcaag aatatgcttt ggaaatatgg gagctggatt
    17221 ggttaccttt ggaacattgg tttctgttgg tggaatttgt atgtgtggtg cttttgtaaa
    17281 tccaagaagt tgtgtagctt tagctgatga taaaatgtta ccgagaatat ttgcaagaaa
    17341 agataaaaaa ggtacaccat atgtagcgat aatagctact atgttaatca ctataccaat
    17401 agctctatct ggaagttttg ctgaattagc agcaataagt gcagttgcta gattcataca
    17461 atacatacca acatctttat ccgtattagt gtttagaaag aaaagaccag aattagtagg
    17521 tacttttaaa actccttttg gagcagtgat tcctctaatt gctgtatgtg ttggaacatg
    17581 gttgttattc caagcatcta tgcatcaatt aattatggga ttaggagctt tggcaattgg
    17641 agtaccatta tactttataa tgaagtctta taatagaaaa atctatggtg aaaatttaaa
    17701 gaaaatagtt taattatgtt ataacaattt attgagttaa aattagacat tggcagaaaa
    17761 ttaaatacaa ataagtatct acaaggctat attaaaatat tattaatttt ttattttaat
    17821 atagccttat attgttatca ataaatgaaa aatattgtaa caacagaaga acaatagtat
    17881 tatgtttata gacaaaaaac ttattaggag gtaaatttat gccaacaata acattaaaaa
    17941 atggtgtaga cgtacttatt agagaaggtg taagagaaga tgctcaaagc attatagatt
    18001 tttataatga agtgggtgga gaaactcatt ttctgtcatt tggaaaggat gaatataaaa
    18061 tatctttgga ggaacaagaa aatgctatag aatcagcaaa agcatctgat aattctgtta
    18121 aactgattgc atttatagat ggtgaaatag ttgggattgc aacgattgat tctaatcaaa
    18181 aagcaaaagg aaagcatgtt ggagtattag gaattgttgt aaaagaaaaa tattggggaa
    18241 ttggattggg taaaaggctt atgttagacc ttatagaatg gtgtaaatca aatggaataa
    18301 ccaagaagat aactcttgtc actaatgaag aaaattataa tgctatagga ttatacaaaa
    18361 aagttggatt tgaagtagaa agcatactaa agaaagaatg ttattataat ggtgtttata
    18421 ctgatttaat aggaatgtca cttttattgg gaatataatt agtgtacatt ttatctcgaa
    18481 aaaagtatat taagtatggt ataattaaaa aagaatataa cttaatataa ttttggagga
    18541 cgaattatga aaaatctgtg taaaatatca gatgattatt taaacagcaa gcttaagtat
    18601 ttaaagttat tatcaaaaca atatccaagt atatcaaaag caagtacaga aataataaat
    18661 ttagaggcta tattaaatct tccaaaggga acagagcatt ttataacaga tgtacatggt
    18721 gaatatgaac catttgtaca tgtattaaaa aatggttctg gtgtaataaa gagaaaaata
    18781 gaagaattgt tttcaaatac aataagagac agcgaaaaaa aaatgttagc aactcttgta
    18841 tattatcctg aacaaaagtt agacctaata attaagcaag aggaaaatat agatgatttt
    18901 tatagaataa atatttatag acttatagaa ctttgtaaat atgcttctag caaatatact
    18961 aggtctaaag ttagaaaact gttaccagaa aattttaaat acataataga agaacttttg
    19021 catgagcatg ttaaaagtga acataaagaa gagtattata aaagtatagt tgaaacaata
    19081 gttgacatag gaatagctaa ggagtttata attgctattt ctacagttat acaaaaattg
    19141 gttgtagata gacttcatgt aataggagat atttatgata gaggtccaag accagatata
    19201 attgtcgata agcttattga acatcattgt gtagatattc aatggggaaa tcatgatata
    19261 ttgtggatgg gtgcagcctc aggtgaaaaa acttgtatag caaatgcact gaggatatca
    19321 gctagatatg cgaatttaga cattgtagaa gacatatatg gaataaatct attgccttta
    19381 gccacttttg ctatagaaat gtataaagat gacccttgta aagaatttat tccaaaggtt
    19441 aatgaccaaa gtgtaacaac aacagaaaag tctttaatgg caaaaatgca caaagcaata
    19501 agtataattc aatttaagct tgaaggtgag gttatcagac gaagacctga atttgaaatg
    19561 gaacatagac tcttgcttaa tatgataaac tatgatgaag gtacgattaa tttaaaaggt
    19621 aaaacatata aattaaaaga tacttattta cctacaatag acaaaaaaga cccatataaa
    19681 ttaactatgg aagaaagaaa tgttattgat aaattagtat cttcatttag aggtagtgaa
    19741 aaactacaaa aacatgtttc atttttgttt tctaaaggaa gtatatattt aaaggctaac
    19801 tcaaatttac tgattcatgg atgtgtaccg ttaaatgaag atggaagctt tatgtctatg
    19861 aatataatgg gaaaagagta taaaggtaag gctcttatgg ataagatgga gtctctagca
    19921 agagaaggat ttttctttaa agataaggca gaagaaaaac tgtatggcat ggatattatg
    19981 tggtatttat ggactgggaa atgttcatca ttatttggta aagatgatat gactacattt
    20041 gaaagatact ttatagctga aaaagagact cataaagaaa ataagaaccc atattttaaa
    20101 cttagagaaa atgagatggc ttgtaaaaga ttatttgaag aatttgattt agaacttgat
    20161 gaatctcata taataaatgg tcatgttcct gtggagagta aaaatggaga gagcccaata
    20221 aaagctaatg gtaaaattct cgttatagat ggtggttttt ctagggctta ccaaaaaact
    20281 actggtatag ctggatacac tttgatatac aattctagaa ctttgcaatt agtatcgcat
    20341 gaaccattta attctgctga agaagccatt gcaaatgaaa gtgatatctt atcgacaact
    20401 gttgtagtag agcataaggc aaagagaaaa atggtgagag atactgatga aggaataaag
    20461 atacaagagg aaatagaaga tttaaaacta cttttaatgg cttataagaa agggctaata
    20521 aaagaaatgt agatgttata gaggcacttc atgattgaag tgtctttttg ttaatactta
    20581 ttaaatatat tataaagatt aataatagta gtttatatta tacttactaa gtgaatccaa
    20641 gataaatggg ttcacttttt ttatttataa acattattaa aaaaatatag atgttttggt
    20701 tatattcctt gtcctttata aatagaatta tataaacaaa ggaggaggac aagagatgaa
    20761 taaactttct gatgaaataa taaactctct ttctacaact ataagagaaa aggtagaaaa
    20821 agtctcaaac aataatctga acatagaaga aattaggtta cgctcacaaa aaccattgat
    20881 actaaatgca aactctaaag attattttta caatcaaaaa actatgacac ttgatttaaa
    20941 tcaacaaaac tcatatgtag taacaagaga agatgtagag caaacttttc aaataatatg
    21001 taaatattca atacattcat ttatggatga cataaaaaaa ggattcataa ctttgagagg
    21061 aggtcataga gtagggttag ttggaaaagc aatagtagaa gatggacaag tcaaaaatat
    21121 aaagcatata tcatctttaa atataagggt atcaagggaa attattggat gctcggataa
    21181 gattttaagt catatcataa agggaaaaaa tcagattaat aatactttga taatatcacc
    21241 acctcaatgt ggaaaaacaa ctttaataag agatatagta agaaatttaa gtaatggtaa
    21301 tgaagattat ggatttaaag gtttaaaagt ggctttagta gatgagcgta atgagatagc
    21361 aggagcttat ctaggtgttc cacaaatgga tgtgggaata aggactgata taatagaaac
    21421 ttgcccaaaa gacttgggta ttacaatgct tttaaggtct atgtcaccaa atgttattgt
    21481 gacagatgaa attggaagtg aaaaagaaat aaaggcatta tacacagcat taaatggagg
    21541 tataggatta ataactactg tgcatggaga ttcaatagag gatatacaaa atagaaaaga
    21601 attaaataga cttctggata aagagttatt taaaaaggtt ataattcttt ctgcaaaaag
    21661 aggagcagga acaattgaaa aaatttatga tttagaagaa aagagatggt attttgcaaa
    21721 ttaaaataat tattatagct ttcttgatag gaagtagtta tttgataggt gaacaaatat
    21781 acaaaactta tactcgtaga cataaacaat taaacgattt aataagagtt ttagaaattt
    21841 tgaggatgga tttatctttt ggactttata cattagaaga aatctttaat aggataggtg
    21901 gaaataaaga gttttgtttt tggaaatttt tctatcaaat atcagaagga cttcacaatg
    21961 aacaaagtaa aactttggaa ataataatat cagaaaatat tgatgtctta agtaaagaaa
    22021 cttatctagg caataaagaa attgaggaac tgaaaaatct aatacttact ttagggaaaa
    22081 gcgatataga gtcacaacaa agaatgatag atttatctat agaaaatttg aaaaaacaaa
    22141 cctatgagac aaaggaagat ataaacaaaa aaggcgtatt gtataaaaaa ttagtgacat
    22201 tcataggtat aggtatatgt attatattga tttaatatgg ggaggtttat tatggaaata
    22261 tcattgatat taaaggttgc aggtgtcggt attctaatat cagtattgaa tatgatattg
    22321 gaaaaaacag atagaaaaga ttgggcaggt cttactacat tagcaggtgt aataatcgta
    22381 ttgggtatgg taataacaga aataagtgac ttgtttaata ctgtaagaac aatgtttcaa
    22441 ctttactaaa gaggagggag taatttggaa ataatgcaat taataggaat tgcaatcatt
    22501 tcaactacac tctgtttagt aattaaaaaa gatagaccag agatagcaaa tttcatagct
    22561 ataataactg gagtaataat actattgtca gttatgttta agctaaattt tatagtagat
    22621 agtatacaag atttggctaa caaggctaac atcccgacta tgtatatatc attaataata
    22681 aaattaattg ggatagcata cttaatggaa tttgcgatac aactttgtaa agattgtggg
    22741 gaaggaaaca ttgcttcaaa actagaattt ggaggtaaaa taatagttat gtcaatgtct
    22801 ttccctatac ttttgtccat tgtagaaatg gtggtgaata taattccata gatgtgtatc
    22861 ttggaatgaa tataagtata ttagagagct ttcgttgtat tgaggtttat agagtgaaga
    22921 gaggcatggt tgcttatgaa aaaaagattt ttaccaatga ttataggttt tttatttact
    22981 ttcttttttg taaatatctt tgctattatg atatttgcta atgaagtacc aagtagtgaa
    23041 gataaagaat atggtgaaac aaaaaatagc atagataagt atatagatgg tcaattagac
    23101 aagctagata taaatgagat tcaagactat ataaataaag aaatagttat taatgatgtt
    23161 aatttaaaat catttgtaaa agatttaatt agtggagaaa aaaatatctt agatttattc
    23221 aataaagatg gattgaagat attgatgttt gatgaattta aagcaagttt aaaggtagta
    23281 gcagtaattt tggtactggc tctgttatca tctattttaa agagtctgga aaactctttt
    23341 tcatcaggtg cagtaagtca aatagcaaca tatataatat ttatcacaat ggtatcactt
    23401 acacttattg gttttaaaga tgtattacag atttgctatg atgcaataga ccatactgta
    23461 ggtcttatgc aagttataat gccaatatta ataacatttt tacttttaat tggattccca
    23521 ataacgtcga ctactttgaa tcctatcttc ataggaggag taacatttat aaatgtattt
    23581 tttaaaaatt ttctatttgt atctattact gttgcatttg gaattttaat aataaacaat
    23641 ttatcaaaaa atataaggct aaagagattt ttctcctttg taaaacaaat aaattatgtt
    23701 tctattggag ctatgtttac tgtttactta ggtcttgtat ccatacaagg actgtatgta
    23761 accagttttg ataaattcag tgtaaagact gctaaatttg ctataggtaa ttttattcct
    23821 gttgtaggtg gatttgtatc tgactctgtg gatattttat tatcttcatc tcagcttata
    23881 aaaaatatat ttggaggaat agggcttatc ttacttgttg gaatttgctt acttccagta
    23941 ataaaaattt tatcagtaat agtagtgtac aaattagcag caataattgt agagcctgtt
    24001 ggagaggatg gaatatctaa ttttttaaat gaagtggcaa atttaatgat aataatgcta
    24061 gcatcagtaa ttgctataac agtaatgttc tttgtaactg tagctatctt gacctcgata
    24121 agtgtcgtaa gtcaagggta atttatatat cagtgaaaaa taattacatt taatctaatg
    24181 tttttagaga ggaggatgct atgttagagg gcataaaggc atggatagtt agtgttttga
    24241 taggagcatt tatagtaaat atagtagata tgattttacc aagttctaag ataaaacctt
    24301 atgtcaacct agttttaaac tttatgtttg tatttattgt aataactcca gtagtaggat
    24361 ttttttcaaa agatatgagt ttagaagata gaatattaaa atctatggga aactacaaca
    24421 agcagtatgt agatagtaca aatgctttgg caaaagagac tggaaataat agtctatcca
    24481 agggatatga ggatgggtta aaagaagtac ttaaattaaa acttgatgaa tatggttatg
    24541 atttagaaga tatagagctt aatggggcaa atataaataa tataaaaata aaagaaaaga
    24601 ataatagtgc aaaaagtaat aacagttcca atgaagaaaa taaaaataat agtactaaaa
    24661 gagaggaaga aaacataaac tctaatgaca aagaaaattc taaacaagtc ttcaaaaaag
    24721 gtacagagta tgggttgaac ctaaatgaag aaaaattaaa aaacgactta ataaaggtgc
    24781 tggatgtttc tatagaagat atacaaattg ataaatagga gttgggagaa tgttgaaaaa
    24841 cttaaatgac aaagataaga gaaaggtata ttcactaata actatagcag gaatatgtgt
    24901 ggtttcatta gtattattat cttgttttcc tagtagcaaa agcgataaac aagttggaaa
    24961 aacagagact aataaaaatg ctgaaaagca agtcactaaa gaacaagaaa aagatgattt
    25021 agagtcaaaa ttaacagcaa tactatctaa aattgatggt gcaggagacg ttgatgtaat
    25081 ggttacattt gaatcaagtg aagaaataca acctgctttt aattcaaata atacaacaga
    25141 aacaacagag gaaaaagatg cacaaggtgg agaaagaaca gttacgactt ctagtgaaaa
    25201 taaaacaatg ataacttcaa attcaagtga ccctgtagtt ataaaaacta cagagccaaa
    25261 gataaaggga gtgatagtgg tagcaagtgg agcaagtgac ccaagtgtta aagagacact
    25321 ttatagtgca gtacaaacat cattacaagt agctggtcat caagtagaaa tatattctaa
    25381 ataaatacat ataaccgggg ggataaatga atgaagttta attataaggg aagaggattt
    25441 gtaataataa ctttaactgc aatgttagtg gtagtaggaa cagtgaatta tcaactaagt
    25501 aagaaatctt tattagaaac atcaaaagag tttaaggcat atgaagaagc acagttgcaa
    25561 aaaaatactg atgagagtga tagtagtaag acagaggatt ctagtaatat agataaacaa
    25621 gatggtaagg aaagtgctga tattgatata gttgatagca aagctagtaa agtaaaagaa
    25681 aaggcaactg agacaagtaa ggaaataaaa gctcaattat catcagagaa aaatatgaaa
    25741 aaggcatctt atattttaga tatgaaaatg aatagagaaa aacaaagaaa tgagttagtt
    25801 caagatttaa atgaaatgat aaataatcca tctacaacag aagaatctag aaaagaagct
    25861 tctaatatga aattaaatat agttaaaatt caagaaaaag aacttcaaat agaaaacctt
    25921 ttgagtacaa aagggtatga agaagcgtta gtttatataa gcgataacaa agttaatgta
    25981 gttgtaaatg aagctaaatt agaaaagaaa gatgcagcaa aaatatttga tttagttgca
    26041 gaacaagcca atgtgaagta tgaaaatatt aaacttacaa ataataatag taataagtaa
    26101 agtataaacc tcagaattaa ctgaggttat tttcttgcaa agtagacttg tatttggtat
    26161 tattatactt ataaaaatag ttccttaaaa tcaggaggta agttttaata tggaagataa
    26221 taaatttgga caagtaaaaa tatctaatga tgtaatagct acaatagctg gtttagcagc
    26281 attagaagtt gaaggcatag aaactacagc aacattaaca gataagttgt taaaaaataa
    26341 tggagtaaaa atacaaatcg aagaagagga tgttaattta gatgtaatgg ttacgataaa
    26401 gtatggaatg tcaataccag atacagcttt taaagttcaa gaaaatgtaa aaaatactgt
    26461 tgagacaatg acaggactga aggtttctca agtaaatata cacattcaag gaatcagctt
    26521 taagaaggat aaagttgata aagaagaagc aaaagcaact aagaaaaatt aatattagcc
    26581 ctctgaaaag tcagagggtt ttttgctggt aattgatttt atatggataa ttaaacttaa
    26641 aggagaattt tgattgatga aaaaagatag ggcacaaaag agtacaacaa gagaatatat
    26701 aatgaaactt atataccaaa taaatattaa taaagaagat tttgaaactt tagaagataa
    26761 agtagataat tttttaaaag ataattcaga acatattata aatagatata aagaacttgc
    26821 acttcaatac tcaaaaaata caaatttaaa gttagaagat actgaaattg aagatgttat
    26881 agacaaaaaa tatataaata cagtatgcaa agcattgaaa gaaaatcatg acaaaataga
    26941 tgagttaata aataagcatg ctaaaaactg gacagtagat agaatgccaa aggtagatgt
    27001 ttctatactt agattgtctg tgtgtgaaat actttattta gacactccta ataaagtatc
    27061 aataaatgaa gcagtagaac ttgcaaaaat atattgtgat gataagtctc ctaaatttat
    27121 aaatggtata ttgggaagtg ttgttgatga aattgggaaa taatataata attggaattg
    27181 atactagctg ctatactact tctatagcag ctatctcttt agataagaaa gttatattca
    27241 atgaaaaaat aatgttagaa gtaagggata attcaaaagg tttaaggcaa agtgaagctg
    27301 tatttcaaca tatcaataat ttagggattc ttagtgatag gataaaatct tttaaagata
    27361 agtttaatgt agaaggagtt tgctcatcaa aaaaaccgag acctgttgaa aactcatata
    27421 tgcctgtatt caatgtaggt cataattttg gaaaattatt gtctagtata tatggttgta
    27481 ggttttatga gactactcat caagagaatc atattgaagc aagtttatta aatagtaaat
    27541 taaaaaataa taataaattt atttctgttc atatgtctgg tggtactaca gagatacttt
    27601 taacaagcaa acaagatagt catcacaatg tatgtgatac taatttaggt aaaattgcca
    27661 aaataagtat taaaaaagat gataaaagta agttatacaa taattttgga tataatatag
    27721 atataattgg aggaagtaaa gatattagct ttggtcaact tatagataga gtaggaatta
    27781 agcttggata taaatttcct tcaggaaagt atttggatga aaatgcttta aattgtaatc
    27841 ttaaaataga aagtggtctg aaaacttctg taagagatgg gtatatgaat ttatcaggat
    27901 tagaaaatca ggtaaataag attataaatg ataatggata taatactaat caaaaagaat
    27961 atatatctaa attggtatta gattcagtag ttagaaatat gtttaaatct ttagtgtatt
    28021 tatgtgaaac ttataatgta aatgaagtag tatttgctgg tggagtttct gcaagtaagt
    28081 atattcttag agaattaagc gtgaagttaa gaaaaaagca tatagaagct tattttacag
    28141 aaccacaata ttcaactgat aatgctgtcg ggtgtgctat aataggattg aataactttt
    28201 taggagaaag agtatgaaac taagagcttt agatataagt gaagcaaact catatattaa
    28261 aagaatttta ataaacgacc caatactttc taatcttaaa gttaaaggag aaatttctaa
    28321 ttttaaggtt catagtagtg ggaatgtata tttatcactt aaagatgaaa catcaaagtt
    28381 aaactgtgta atttttaaaa gtaattttaa tcgcaattta aaattagata atggtgtaaa
    28441 aattatagct aatggatata tatcagtcta tgaaagagat ggtgcgtatc aattatacat
    28501 taatgaaatt gaaatagagg gaataggaaa tttgcacata gagtttaata gattaaagga
    28561 aaaacttaat aaagaaggat tatttgaccc taaatacaag atacctatac ctaaaatgcc
    28621 aaactcaatt ggcgttataa catctccaac aggtgctgtt ataagagata taataaatgt
    28681 aataaaaaga agatatccga aagttaatat aaagctttat ccagtaacgg ttcaaggaga
    28741 taaatcagct gaagaaatct gtgaagcaat tagatttttc aatcatatga agaatgttga
    28801 tacacttata gttggtagag gtgggggttc aatagaagaa ctttggtctt ttaatgatga
    28861 aatggtagca agagaggttt ttaattctca aattccgata atatcagcag ttgggcatga
    28921 aacggatttt actatttgtg actttgtgtc tgatatgaga gccccaacgc catcagcagc
    28981 agctgagatt gctactccat cattggatga tataaactat aaacttggaa acataaaaag
    29041 taggatgagt aagtctctta caaatcaaat agagctagac caatataggt tagaaacagt
    29101 atttaataag ataaataact atttagattc gtacacaata aaagataaag ttatacaatt
    29161 agacaaaata tatgataaaa taatttttgg aatagaaaat aatttaaaat tagaagatga
    29221 aaaattagta aaaattggag cattactcca taatttaagt ccattagcta ctatggatag
    29281 aggttatagt ataactcaaa aaaatggaaa agtaataaat agtataaaag gattaaaaat
    29341 aaaggatagt atagatatag tattaaaaga tggaaattta gaatgtatga tagataaaat
    29401 tgaaaataaa gagggatagt atgaatttaa cctacgaaga agcttataaa agattagaaa
    29461 gtattttaag tgaattagaa tctaaaaatg ctagcttgga tgaatcttta agcctatatg
    29521 aagaaggtat aagcctttat aaacattgta ataagctttt agatgatgct aaattgaaaa
    29581 taagtaaatt taatcaattg ggaatagaag aagactttaa aatagaggag gaataaaatt
    29641 ggaatttaaa caatgtctaa aagaaaaagc aagttttgta gaaaaagtac ttaaagaata
    29701 tatgcctaaa gaagaagggt atcaaaaaac tgttattgaa gctatgaact atagcttaag
    29761 tgctggaggt aaaagattaa gaccaatact tacattggag gcttgtaaaa tagttggagg
    29821 taatgaagat gaagctattc cttttgctat tgcaattgaa atgatacata catattcact
    29881 aatacatgat gatttgccag ctcttgataa tgatgattta agaagaggaa gaccaacaaa
    29941 tcataaagta tatggagaag ctatggggat tttagcagga gatgctttgt taaactatgc
    30001 ttttgaagta atgcttgcag gctcaatcaa taaagaaaat ccagaaaagt atctaaaagc
    30061 tataaatgaa attgccaaag gtgctggtat atatggaatg ataggtggtc aagtagtaga
    30121 tgtagaaagc gaaaataaac aaattgaaaa agaaaaatta gattatatac atatgaataa
    30181 aactgctgca atgatggtag gctgtatgag agcaggagct actataggtg gagcaaattc
    30241 tgaacaaatg gaagaaataa ctaaatatgc aaaaaatatc ggattatcat tccagatagt
    30301 agatgatata ctagacatag taggagatga agctaagtta ggtaaaaagg ttggaagtga
    30361 tatagaaaat cataagtcaa catacccatc tcttttaggt ttagataaat ctaaagaaat
    30421 tgcacataat ttgattgatg aagcaaagaa aagtatagaa aaattatctg atgatgtaga
    30481 ttttttaaag ggattggctg aatatataat agatagagaa tactaaaata tacagctaaa
    30541 atcgaggagg gaaaaatgga ttttttttcg gagattttta acaatggggc tttgggaata
    30601 agtctgattg cttgtttctt agcacaattt ataaaaatat ttactggtaa ggaaaaaaga
    30661 attgagttat caagaatact tatttcagga ggtatgccta gttcccatag ttcttttgtt
    30721 acaagtttag ctacagtggt aggtattgaa aaagggttta attctactga ttttgctata
    30781 attaccgtgc ttgccttaat tataatgtat gatgcagcag gtgttagacg ggctgttggt
    30841 aaacaagcta ctatattaaa tcaaatggtg gcagatatac agcatggaaa gcatatagag
    30901 cagaagaaat taaaagaatt aataggtcat acaccactag aagtttggtt tggagcttta
    30961 cttggaatag taacagcttt aatattgatg taaattgaaa atttacagaa taaaggtgag
    31021 gataatatgt ataaatattt agataaagta aattctccaa aggatataaa aaatatgagt
    31081 atagaagaaa tggatttact tgcaaaggat ataagaaagt tcttagtaaa atctgtttca
    31141 aagacaggag gacacttagc ttctaatcta ggagtggttg agttaacatt agcacttcat
    31201 aaagtatttg atagccctaa agataagatt gtatgggatg tagggcatca atcttatgtt
    31261 cataaaatag ttactggaag aaaagattgt tttgtatctt tgagacagtt taatggacta
    31321 agtggttttc caaaggagaa tgaaagtcct catgatattt ttgatacagg acatagcagt
    31381 acatctattt ctatagctac aggtattgcc tgtgctaggg atataaaaaa agaaaactat
    31441 agtgttatat ctgtgatagg agatggttct ataacaggag gtatggcact tgaagcttta
    31501 aatcaattag gatatataga taccaatatg atagtaatcc ttaatgataa tgagatgtcc
    31561 atagataaaa atgttggagg aatgtctaaa tatctgtcaa gtattataag aaactcaaca
    31621 gttgagaaaa tgacagatga agttgataaa attttaaatg ttacacaaac aggtgaaatt
    31681 ttatctaaga ctgcacatag atttaaagat aaactaatgt atagcttttc tcctcaggac
    31741 tgttcatttt ttgattcatt aggtataaga tattatggac ctatagatgg ccataatact
    31801 aaagaactaa tagatatatt gagaaaagct aaacacaaaa aagggcctgt tcttttacat
    31861 gtaattacga aaaaaggaaa agggtacaga tttgcagaag aacaacctga taaatatcat
    31921 ggagtatcaa agtttgatat aaagacaggt gttacatctg caaaagttaa gtctatgtca
    31981 attagtgttg gagaaaaact tgttgatatg gctaatagta atgaaaatat agtagcaata
    32041 acagctgcaa tgccatctgg tacagggtta aacctgtttg aaagtgcata tccaaaaaga
    32101 tattatgatg ttggtatagc tgaacaacat gcaacaggat ttgcagcagg tcttgcaaaa
    32161 aatggtatga aaccttactt tgctgtgtac tcatcatttt tgcaaagggc atatgaccaa
    32221 gttattcatg atgtatgcat aacaaaaaag ccagttactt ttcttataga tagagctgga
    32281 ttagttggaa atgatggaga aactcatcat ggtatgtttg atttaagtta cttaaattct
    32341 attccaaata ttgtagtaat ggctccaaaa gacacaagag agatggagct tatgatggat
    32401 ttgtcattaa aattagattg tccactagca attagatacc caagaggaag tagttattac
    32461 ctagataagg gagagtatgg agagattgta ctgggtaaat atgaggtatt agatgacggt
    32521 caagatacag tcatactttg tattggaagt atggtaaaac atgctttaga ggctaaagaa
    32581 atattatcaa gagaaggaat aaatccgaca atagttaatg ctagattctt aaagccaatt
    32641 gatgaaggta tgttaaaagc attattaaaa aatcataaaa atgtagttac tatagaggat
    32701 aatattgtaa ctggaggatt tggaagtaga ataaataagt ttattataga taatgaatat
    32761 aacgtaaata tacttaatat agcaatacca gaagaatttg taaaacatgg caatatagat
    32821 gaattatatg attttgtagg attatcgcca aaaagtattg ctgataaaat aaggaaatta
    32881 gttattgagt aaaaatctaa tgtagatatt gtaaattaaa atttatataa aatatagaat
    32941 ttatcaatta gaataaatat caagtgtgtt acttcaagct actttagtta tcattaaagt
    33001 agcttttaac agaaaggtaa ttgtatgaaa aaaagaatag atttattatt agtagaacaa
    33061 gggtactttg aaagtagaga aagagccaaa aaagcaataa tggctggatt ggtttttgtt
    33121 gataatcaaa ggtgtgataa ggctggtaca gaagtaaaag aagattgtag tatagaagta
    33181 aaaggaaatc caataccata tgtcagtaga ggtggtttaa agcttgaaaa agcaatgaaa
    33241 aactttgatt tgactattga tggaaaggta tgtatggata taggggcatc cacaggtggt
    33301 tttacagatt gtatgctcaa aaatggagca ataaaagtat tttctataga tgttggttat
    33361 ggtcaacttg catggaaatt gagacaagat gatagggttg tgtgtatgga aagaaccaat
    33421 ataagaaatg tgacaataga agatacaaag cagtttgctg attttgcgtc aattgatgtg
    33481 tcttttatat cgttaaagtt agtacttcca aaggcaaagg agttagtacg tcatgatggg
    33541 gaagttgtcg ctttaattaa acctcaattt gaagcaggaa gagaaaaagt aggtaaaaaa
    33601 ggagtagtta gagaaaaatc tacacatata gaagttataa aaatgatttc tgatttctca
    33661 gtcgaaaatg gatttgagat tttagggtta gatttttctc ctattaaagg tcctgaaggt
    33721 aatatagaat atctaattca tttgagaaat ggaaatgaag gatatgaatt tgatggtgag
    33781 acatacaata acaaaatagt agaagttgtt gaagcttctc ataatttaga caaataggtt
    33841 aaaagggggc ttagatttga tcctcgaatt atatatgaaa aattgtgcct tagttgaaga
    33901 actaaggttg aatattgata aaaacttaaa tatacttaca ggtgaaactg gttcaggaaa
    33961 atctataata atagatgcac ttggactttg tttaggtgat aaatatgata ggtctttttt
    34021 aagaaaagga acagataagg gacttgttga ggctgtattt ttttcagaca atagatatct
    34081 taaaaaaata ttagaagaaa atgatataag tatggaagat gataatttat tggtaataac
    34141 taggttgata tactcagatg gtaagagtac tgctagagta aatggaagga ctgttaaagt
    34201 gtctcttctt aaagaaattg catctacact tatagatata catggtcaac atcaaaatca
    34261 agctttgttt aataaagata ctcatttaaa atttctagac ctttttggag aaaatgaatt
    34321 ggaagagttt aaaattgctt ataaaaatgt gtaccataag tatagtgaag taaaaaaagc
    34381 tttaaattgc ttgacagaaa ataaagatga gatgcaaatt caacgagaaa tagatttatt
    34441 aagatttcaa ataaatgaaa ttgaagctgc aaatctaaat aagaatgaat atgaagattt
    34501 attaaaacaa agagaagttt atagaaatag tgaaaagata tataataatc taaattcgag
    34561 ttatagcaag ttacataatg gtgagtataa tgttattgac ctaataggtc ttgcatctaa
    34621 ggaacttaat gacatatcta aatatgacag tgttttaagt gaatatagtg atactgtaga
    34681 aagaatcatg tatgagttac aagacatatc tggtgagata agaaattaca aagataatat
    34741 agactttgaa ccttatgagc tagagcagat tgagcttaga atcgacgaga ttaataactt
    34801 aagaagaaaa tatggagaca gtatagatga tatatttgag tattatggaa aaattaaaga
    34861 tagattagat gaaatattaa acagagatga aagagttgaa cagttaagaa gcaaactgat
    34921 gaatatagaa gaagatttaa aaataaaagc cagtaaattg actaaagcaa gaatagaagt
    34981 agcaactact ttagaaaaaa tacttcttga tgaattaaaa agcttaaata tgaaaaatgt
    35041 aatgttcaag gttaattttg aaaaaggtcc atttacttta gatggagtag atgatattga
    35101 atttatgata tcttttaact taggagaaga tattaagcct atatataaag ttgcatcagg
    35161 tggtgagatg tccagattta tgttagcgtt taaaaccata ttggctgata tagatgatat
    35221 tgatacatta gtgtttgacg agatagatac tggaataagt gggatagcag cacagatagt
    35281 tggtgagaaa ttgagtgata tagcaaagaa aaaacagata atatgtataa ctcatttacc
    35341 tcaaattgct gcaaatgcag acactcatta ttgtatagag aaagatactt caaataatag
    35401 gacgtttacc aatgttagta agttaaatga atctcaacga aaaaatgaaa ttgccagact
    35461 tatagcaggt aacaatataa cagaaaaaac tattgaacat gcaagtgaaa ttattgagtt
    35521 ggctaaaaaa tgttagtgaa aaacaagcat tttaataggt gtaaagaatt tttatagaaa
    35581 tgctttacat ctatttttta tgatgaaatt attttaaaat atctagtgcc tatattgaag
    35641 aaaagtagat ttaaaaatag aagaaaattt gtataaattt gtaactttga aatttctaaa
    35701 tgcttaagta taatattatt tattaaggta gtagtgaggg ggacataaaa tgaataaaaa
    35761 agatactatg cacttaattt ttacaattgt aatttttatt ttattatggt atattgtggt
    35821 agcagtaagt gagattaata aaaatattga aagttgtagt aataaattag attatttgtc
    35881 acaagagatt atgagtacta aatcggatat tagtaatact ataaatgaag aaatgtcaaa
    35941 aagttatata acaaagagta ttgacttaaa agtaaaaaaa atcgagaaaa aagagtgtgt
    36001 tattgatgta gatgttcaat taagtaaact tggaaaaaat ggaaaagtat tttttatgta
    36061 taaagaagat agtgataaat ggaatgaaac tgagatgaca aaacatggag aattatcata
    36121 tgcatgtgaa attaagataa atacaggaag tgaatatgac tataaagtag ttacaagtgg
    36181 agctatatct gaaagttctg atgtgcaaaa attgactaaa tctgattata tgcctgaaca
    36241 acccatattt aattctggga taagagataa tagagaatac tttattgaaa tagtagaaaa
    36301 tagtaatctt ttcaatcatg agagtgaaaa gtcaaaaaat aaagtttatg ataatattag
    36361 attagaaaaa gtagatatta ttgtaaatga aggaaaaaaa gacactatat acaaagccaa
    36421 attagctgaa ggcttagatg atggcaaaac taataattca aatagaaatc aagtgaatta
    36481 tgaagtaagt tttcctaaaa gggatattga tgacataatt tatattaaag ctaaacttac
    36541 atacaataat ggagtagaat atacaaagga tataactgaa gatataacta tgggattaca
    36601 agatttagaa aaataaatac atatttaaat aaatattcaa atagatttgt tcaataaaat
    36661 agagtataaa taaaactgta gtagttttac ttatactcta ttaaaaaatt ataattttaa
    36721 ttccatatat ctagcccctt caattggatt ataagtataa ggttctatat cataaaaacc
    36781 aaacttttca tataatcctt gtgcactttt catgcttgga agagtatcta atctcatgtg
    36841 tgtatatcca aattttttag cttcttcaat gatttcttcc aatagtattt ttcctatttt
    36901 taatcctcta aattgatttc ttacatataa tctttttaat tcacatattt taccttcaag
    36961 tttttttaaa gcaacacaac ctgccaaatt ttcatcaaca aatgccaata tcaatgagcc
    37021 tgaaggtttt ttatattttc ctggaagtgt ttttaattca ttattaaagt cttgaaaaca
    37081 taagtctata tttagagaat ttgaatattc agtaaataga agttttacgt tttcaaggtt
    37141 ttctaaatct ttttcttcaa caaattgtaa agttatattt tgagccattt tatccgccat
    37201 tatataaaca tgtttatata atgacttcac ctccttgata tatgtatatt tttcttaata
    37261 tcaattagat tttaatacca ttactttttc accaccatag tcaagctctc cattaaactc
    37321 aaatccaaaa tctttataaa gcttaatagc cattttattg tcttcgaata tactaagata
    37381 tagttcatca cagttatatt catttttaag atgatttaca agaaaatcta atgatgcttt
    37441 tccatatcct ttaccttgat attttgaatc aatcataaat ctatcaagcc aaactctatc
    37501 atcttctaaa cgaccataca tagcaaatcc tattaaagaa ttttcatcat atattccaac
    37561 aggtacccaa gctgttataa atttagattc agcaatagaa agtgcattgc tttctatata
    37621 atctaactga cttttatcta cagacaaact aactaaatca tgccaattat ttatgtctgc
    37681 aattcttaca tttaatttca agatattacc gcccttcaaa ctttttgtta caatctaata
    37741 ctatcatatt atgtattaaa aatcacttat taagatttta aataaacttt ttaaatgaat
    37801 aaattctcag gaaaaaggaa ttataaatac ataataatac agcataggag tagatggata
    37861 tatgaacaaa ataaataaaa atttcaaaaa tatttggata atattaggag ctgtatcgat
    37921 ttttaccatg gttataattt ttaataaaaa ttgtaatgtt aataaagaga ctcaaataga
    37981 agcttttaca attaagaatc atgataaaaa atttgtatat cctatgggaa atataattgg
    38041 agtaaaagca aatacagatg gtgtattggt cttaggatat gaggaagagg atgtagacta
    38101 tattggaggc atacaaatcg gtgataatat agtaaaaata gataataaaa ggataaaaaa
    38161 tagtcaagat gtatcagaga ttttgaataa gattaaaaaa agtaaagttg aagtgacttt
    38221 tgagagaaaa aataagtata aaacagaagt aatagagact aaaaaagaaa atggcaagta
    38281 taaacttgga ttatgggtaa gagataaaat atctggaatt ggaactatga ctttttacga
    38341 ccctagtatg gaaaagttca aagcaatcgg tcatgccata aaagattcgg atactaatga
    38401 actattaaag ataaagcaag gatatatata taagccagaa caattaaaaa tagtaaaagc
    38461 tagtaatgaa aaagtaggta aaataaaagg agattttaat gacagcaatt taatgggtaa
    38521 tttctcaaat aattcagagt taggtataag tggtaatatt acagaaaacc ataataaaga
    38581 gtttaatgta gcaaataaag aaaagcaatt aatagaagtt ggaagaccgc aagatgtaaa
    38641 aataggagat gcagtaattc tttttgagga taaaaacaaa aatataacaa gctatgatat
    38701 aaaaatagaa agtatagtat atgataaagg aaattataga gatatggtaa taaaagtagt
    38761 agatgacaag ttattggaat acacaggagg tatcgtacag gggatgagtg gagctccaat
    38821 aatacaaaat aataaaatta ttggtgcaat aactcatgtt tttagagata atccgaaaaa
    38881 aggatatggt atttttatag atgaaatgat aaaattgtag gtgaggcatt aaaaaatttt
    38941 attattttat caattatcta ggaggaatat aattttggag tgtcgaatat gctttagagt
    39001 agataattag gaagcaattg tgtaaaaagt ttagttttct gtaataagaa gatgtttttt
    39061 aatgggggga tttttagtgg aaaaaatcaa aatagtttta gcagatgaca ataaggattt
    39121 ttgtcaggta ttaaaagagt atttgtctaa tgaagatgat atcgatatat taggcatagc
    39181 taaggatgga attgaagcat tagacttagt aaaaaagaca caaccggact tattaatact
    39241 agatgtaata atgccacatc tagatggatt aggtgtaatt gaaaaattga atactatgga
    39301 tatacctaaa atgccaaaaa taatagtact atcagcagta ggtcaagata agataactca
    39361 aagcgcaata aatctaggag cagattacta catagtaaag ccatttgatt ttgttgtgtt
    39421 tataaacaga attagagagc tagtttctaa tagagttaca caagttgagc caaaacctag
    39481 accagttcaa gagactcaaa tgactagaag tgattttgtt aagaatgtag gaaatataga
    39541 gacagaaatc acaaatataa tacatgaaat aggagtacca gctcatataa aaggatattt
    39601 atatttaaga gaagcaataa aaatggttat tgataatgtt gagcttttag gtgcagtaac
    39661 aaaagagtta tatccaagta tagctaaaaa attcaataca acaccaagta gagttgaaag
    39721 agcaataaga catgcaatag aggttgcatg gagtagagga aaagttgaca caataaatca
    39781 attatttgga tatacggtac acaatactaa aggaaaacca actaattcag aatttatagc
    39841 aatgattgct gataaattaa gactagaaca tagtatggtt aaataaacaa gacataaaaa
    39901 gtaaggcttt ttaattaagg cattggctat aaatgcgtat tacaagcagc gaaacggtat
    39961 aaccactagg gttataccgt ttcgctattt taataaatat aaaaattttc tttattattt
    40021 gcttactata tcaatataat aattttatta tactatggat atagtatgtg tctttacaag
    40081 ttgtaaactg acagtggttt attttttaat ataaatattg actttgatgc aggtaaactt
    40141 tgtattttta agcgtattat ggaatatgtt aaataaaaaa atgatgaaat atagtattgt
    40201 aaatgccaaa gatgcaaaac aaacttaaaa catttatttt attgttaagt aatgctataa
    40261 tataatgtga ttttaataat gatagtggag gtttaaatat gagagtcgag gcccctataa
    40321 aagtagatcg aaaaaccaaa aaacttgcta aaagagttga aagtggggaa atagcagtta
    40381 taaatcatat agacatagat gaagttgctg caaactcttt agtagaagct aaaataaaac
    40441 ttgtcataaa tgcggctcct tctataagtg gtaggtatcc caataaaggt ccagggatat
    40501 taactgaaaa taatatacta ataattgata atgttggaga agagttattt gaagaattaa
    40561 aagaaggaga aactatagaa gttgtagatg gaaagatata tagaaaagga aaatttttag
    40621 gtgcaggaga agttttagac aagtatgagg tttcacacca aataaaggct gcctatgaga
    40681 atttagctgt agaattggat agatttatag ataatactat agattatgct aaaaaagaaa
    40741 aaggatttat acttggagaa gtagaaattc caaaagtgaa aactaattat gctaataaac
    40801 atgtattgat agttgtaaga ggtcaggact acaaagaaga tttaagtact atgctttctt
    40861 atatagaaga ggtaaagcca gtattggttg gtgttgatgg aggagcagac gctttaattg
    40921 agtttggata tactccagat gtgattgtag gtgatatgga tagtgtaagt gatgaagctt
    40981 taaaaaaggc tagtgaaata gtagtacatg cttatacaga tggaagagca ccaggtctta
    41041 aaagggttga ggaattaggc ttagatgcag ttgtatttcc tgcaccagga actagtgaag
    41101 atatagctat gcttatcgct tatgagtata aggctgagtt aatagtagca gttggtacac
    41161 attcaaatat gattgatttt cttgaaaaag gaagaaaagg tatggcaagt acttttttag
    41221 ttagactaaa aataggttca aagcttatag atgctaaagg cgttaatttg ttatacagaa
    41281 gtaaattaaa aataaagtac atatgggcat taatagcaac agcattattt cctgtgttgg
    41341 tagttgcatc attgtcacca ggagttcaac agttcataca actaatgcaa ttaaagttta
    41401 gagtattatt acagatgtaa attttaggag gataaatatg catattaata tgaaatatta
    41461 tatagtcact ataggggcta tatttatagc tttgggtata ggcatgttag taggatttaa
    41521 tttaaataat aatcaacaat taagtgaaca acaagcaaat ataataaatg atttggatga
    41581 taagtttgat atcttaaaag aaaaaaatga taaacttgac agtgacttag caagtgtaaa
    41641 tagagattat gaagaggcag ttaattttat aaataaaaat gtagataaga ttttagctgg
    41701 ttcgctgaat ggtaagagta taggaataat atctgctaat gaaaatgacg attatacaaa
    41761 aaatatagaa gacataataa ataaatctaa tggaagcatt gcatttaata taatattaaa
    41821 agaaaacata acaaatccag aaaagttaaa ggaaatgtca actaaactag gtacagatgt
    41881 aaaaaatgca aatgatgcag ttaattatat aattgacact cttaaaaagg aagatgcaag
    41941 tgacatttta acatatcttc aagaactaga tgtaataaag tttaatttta ttggagatac
    42001 atatctaaaa tatgattccg ttgtaatagc tggtggaaat gatgcaaaag acagtgcaaa
    42061 acagtttgaa aaaatagaaa aatttgtagt ttcaaaactt aaatcagaga ataaatattt
    42121 agtagaagtt caaaatacag gagttaaaac ttcttatgta gaactatatt ctaaaaacaa
    42181 agttgcaact atagacaata ttgatgaagg tataggaagt atatctctag cgattcttct
    42241 acaacaaggt aatatagttg gtaattttgg tagattggat actgcaactt cattgttgcc
    42301 atctattaaa tagaaataat tttaaggaga gaaaaaatgg attcttatat aagtataata
    42361 ataccagctt ataatgaaga aagtagaata aaatatacat tagaaagcat attggacatt
    42421 aagcaaataa atgaaataat agttgtggat gatggttcaa ttgacaatac tgctaaggtt
    42481 gtatctgagc tagaaaatga aaagattaaa ttttttaagt tagataaaaa tagaggtaaa
    42541 ggatatgctt taaattatgg tatcaaatta gcaatgaaaa atgcagatat tataggtttt
    42601 ctggatggag atttaggaag ttctgctaaa gaagttgaaa aattaatagt acctatatta
    42661 aatggtgagg cggatgttac aatagccaag tttcctcctg caaagagaaa aggaggtttg
    42721 ggatttgtaa aaggtttagc taaaaattct gtgtttgaga tgactggtgt tgagttagat
    42781 gcaactttgt caggtcaaag aatatttaaa agagaagttt tagaaaagtt tgatgaaata
    42841 ccttttggat atggagtaga agttggaatg accattgata tattaaagta tggatttaaa
    42901 attaaagaag tattagtaaa tatgactcat aatgaaacag gaagaaattt aaaaggtttt
    42961 attcatagag ggaaacagta ttatcacata aaaaaggttt taggacaaaa aaagaaagaa
    43021 tggaggtaat atcttagtga aagattatat actttatacc attttattat taactggatt
    43081 atttggaacc tatgctgtaa ttccactatt taaaaattta ttgataaatg gaaatgtact
    43141 tagaccaaac tataaaaaag atatgatacc agtaagtatg ggaattgtat ttttgcctat
    43201 gattgtaatc aatggaataa tacttgcttt ctttactaca gaatttaagg atttagcgta
    43261 tatatttatg tttatgtttg gtatgatatc catgtttttt gcagggatac tagatgatgt
    43321 cattggaaat agagatgtaa gtggtttaaa ggggcatttt aaaagtttat ttaaaggaag
    43381 tcttacaaca ggaggtttta aagctttatt tggtggattt gtagggctta tagtttctgt
    43441 tgctatatca aaagacataa ttgatataat agtaaataca ctaataatag ctctatcaac
    43501 taatttaatg aatcttttgg atttaagacc aggaagggca ataaaagcat atttattcat
    43561 aatggtaatt atatttttaa cattgacagg atttgttcaa gttcttccat tacttatagt
    43621 accaaatgta cttgcatact ttaattatga tttaaaagca agggctatga tgggagatac
    43681 tggttcaaac gttcttggaa tatctattgg aatgctcatt tcttttgggt atccatttaa
    43741 cattagatta ggatggttaa tatttgtagt tattatgcat atattcactg agaaatactc
    43801 attaactaag ataatagaaa aaaataaact attaaatttt atagataaat tgggaaggta
    43861 gtattatgat aagcaaaaga gtggggattg ttgaatcaat agtaagtcaa actgaaactc
    43921 tagatgatat aagagtaaat ataaatggag aaatacaaaa ggcatataat tatccaaaaa
    43981 tatctggaac tataaatata ggtgacgaag tggttttaaa tacaacagca gtagaactga
    44041 gcttaggtac aggtggatac cattttgtaa taactaattt aaataatata gaatctactt
    44101 taactgaagg tggacatata atgaaactta gatatacgcc tcttcaaata aaagtggatt
    44161 ctgttgagga acaagaaagt atttatcatg ataaatttgc gaattttaaa ggattagaag
    44221 gtctttctgt tgtagtaggg actcttcata gtatgttaac tccatttgca gcttcattta
    44281 aaagaaataa tccaaataaa aaattagtgt atataatgac tgacggagca tctcttccaa
    44341 tttatttaag taaaaatgtg gatactttaa aagaaaaaaa attaatagat agtacaataa
    44401 ctataggaaa tgcgtttggt ggagattatg aatgtataaa tatatatact gcattaatta
    44461 cagcaaaaga aatactaaaa gcggatgcag tatttgtcag tatgggacct ggaattgctg
    44521 ggacaggaac aaaatatgga tttacaggga ttgaacaagg ctctattctt gatgccgtaa
    44581 aaaaacttga aggaagagca attgcaattc caagaattag ttttgctgac aaacgagaaa
    44641 gacatcaagg tatttcacat catagtatga ctgtatttaa agaaattgta aatgtaaatg
    44701 tagacatacc aataactata tatgatgatg aaaaattgaa ctttataaaa gagcaaatca
    44761 atttaaatgg tttagacaaa aagcataata taatttatat agataataat aaaacgaaag
    44821 aggatttaga ctattttaat ctaaaagtaa agagtatggg aagaaattat gaacaagatg
    44881 aggcattttt caaagctgca agcacagcag catattactt aatggaggtt taagatggta
    44941 ttagaagaaa aaactattag tagtgatagg gtttatacag gtaaagtaat tactttaaag
    45001 gttgatactg ttgaaattcc agggcaagga tatcaaaaaa gggagttagt agaagttggt
    45061 ggagcagtag gaatagttgc aatcacagat gataataaag ttgtgttagt taagcaattt
    45121 agaaaaccta tagaaaagcc aatctttgaa attccagctg gtaaactaga aaaaaatgaa
    45181 agtccaaaag aatgtgctga aagagaatta aaagaagaaa ctgggtatag tgcaaaaaat
    45241 attaagctaa tacataaatt tttcacttca gcaggttttt ctaatgagat aatgtttgta
    45301 tatttagcaa caggtcttac tccaggagag aataatttag atgcagatga gtttttagat
    45361 gtctatgaaa ttgagcttga agaagcatat aacatggtat taaaaaatga cgttgaagat
    45421 gcaaaaacat ctattggatt attattagta aaagatatgt ttaaaaatta atatttataa
    45481 aatgaataac cctttgtcct aaagcatatt attttataaa ggacaagggg gaattaaatt
    45541 gagaagaaca tatagaaaaa atgattttag agaattaaat agacaaataa ttattatagg
    45601 attgttgttt atgatgtcta ttgttgttgg gtcttacata aataaaatat tgccaggttc
    45661 tagtaacaat atattaaaca atataaatcc tgctgtagag tattataatc taaatataag
    45721 cataaaagat acagtgatac aaaatttaaa atcagatgca atctttatgg gctcaatagc
    45781 tctattaagt ctatttgtag taacaattcc agcagtgttg gtagcttttg tattaaaagg
    45841 gatgtcaatt ggttatacaa taaatagttg tatattggca ttaaaattta aaagtataaa
    45901 aatgatttta ataatattat ttaaaaattt gattattatt ccatgtgcaa tcatattagc
    45961 attaattagt ttaagttatt ttaaagaaat ggtttatgaa tttaaaaaga aaaatagaaa
    46021 aaatatgcaa tttttaatta agaggtatat actaaatatt ataattataa tagcactatc
    46081 attggggttg caattaatat taaatactgc aagtattggt attattaagt tcctagctaa
    46141 ataaaggagt ttataatgga tattatagag ggatatatag attacataaa agataaaaaa
    46201 aaattatcag agaatacagt ggcatcatac tttatggata taaaaaaata tatggaatat
    46261 ataaatcaaa aaagcattaa actagtagat atagtagaaa atgatattat aagttactta
    46321 ataaagttgg aaaaagataa tgtatcaata gctacaatag cgaggatgat atcctctata
    46381 aaatcttttc atgattattt atttttaaat catatatgta caaataatcc tgcaaaggat
    46441 ataaaaaaac ctaaaataaa aaaagaaaat ataaatatac ttacagaaga agaaattgaa
    46501 agactattaa attttccaaa gttaacaaca cctaaattga ttagggacaa ggctattttt
    46561 gaagtattat atggtactgg tataaaagta tctgaattag tagagatgaa tatagaggat
    46621 attgacctag atattgatta tatatattgt aattcatgtt gtaaaaatca aagagtgata
    46681 ccactttgtg atattacaaa actttactta gaaaagtatc taaaagaagc aagacctaaa
    46741 atggctgtag aaggtgaaaa atcattattc gtaagttcat taggtcaaag gtttacacga
    46801 caaggtcttt ggaaagtcat aaaaaaatat tctaatctag ccaatataga taaaaatata
    46861 aatcctacaa tgcttagaca ttcatttgct attcatttgt taaatgaagg tgctaacatt
    46921 gctgttgtaa gtaaaatttt aggaaatgtt aatttatcta gtttgcaagt atatttaaat
    46981 catatagata aaaacgtgag aagagaaata aaggagaaac atcctagaaa tgatgttgat
    47041 gtagaattac aaaaagcaga agctaaatag actatatagc aaattttata atttagaagg
    47101 agcatattta tatgagcaga gtaatttgga ttgtaattga tagtgttggg ataggagcat
    47161 tgcctgatgc tgaaaaattt ggtgatagta aggatgtaag tacccttgga aatatattta
    47221 aggaatatcc agatattcag attcctaata tgagaaattt aggtatagga aacatagatg
    47281 gtatagattt ttttgaaagt ataaaagaac caataggttg ttttggaaag tgtaaggaaa
    47341 tgtctcaggg aaaagatact actacaggtc attgggaaat gactggtata atagttgaca
    47401 aaccatttaa gacatttgaa catggattct ctaaggagat aatagaagag tttgagaaaa
    47461 aaactggaag aaaagtagta ggaaataaac cagcttcagg aactgtaata atagatgagt
    47521 atggagaaca tcaaataaaa actggagatg taatagtata tacatcagct gatagtgtat
    47581 ttcagattgc tgctaatgaa gaagtaattc ccctagaaga actctataat atgtgcaaaa
    47641 tcgctaggga aataatgatg ggggataatg cagtagctag agtaattgct agaccattca
    47701 taggtaagaa aaaaggtgag tttgtgagga cttcaaatag aagagattat tcactagacc
    47761 catttgaacc aacagttctt gataatataa aagaatcagg acttgatgtt ttagcagttg
    47821 gaaaaataga agatattttt aatggaaaag gaattacaga tgctatccac actaaaagca
    47881 atatggatgg agtggatgaa actttaaatt atatgaaaca agataataaa ggtctaatat
    47941 attcaaatct cgttgatttt gattctaaat atggtcatag gagagaccca gaaggatata
    48001 agaaagctct tgaagagttt gatagtagac ttcctgaaat aatggctaat atgagagaag
    48061 atgatatttt aataatcaat gcagaccatg gaaatgaccc aacatataaa ggtacagacc
    48121 atacaagaga atatatacct gttatgattt atggcaataa gattaaaaaa ggttttaatt
    48181 taggagtaaa agatactttt gctgatattg gagcaactgt tgcagatata ctaaatgtaa
    48241 agttgcctaa acatgggtca agctttaaag aagatttatt ttaaactata aaatgataaa
    48301 aacttagctt aataggatgg agagattgga atgtttgaaa agatagcaca aagtagtaaa
    48361 tttataaatt ctaaaagtga tataaaacct aagattggtt tgatattagg ttctggacta
    48421 ggtgatttag caaatgatat agaagaccct gtcgcaatta aatatagtga aattccaaac
    48481 ttccctgtat caacagtagc aggacatgct ggacaacttg taattggaaa acttgaagga
    48541 aaagaagtta ttgctatgca aggtagattt cattactatg aaggatattc tcagaaagaa
    48601 gctacttttc cagtaagggt tatgaaagaa ttaggagtcg agattcttat agtaactaac
    48661 gctgctggag gcgtaaataa agaatttaaa gcaggagatt tgatgcttat aagagaccat
    48721 ataaacttta gtggaagtaa tccattagta ggaaaaaatg atgacagatt tggtgctaga
    48781 tttccagata tgtcagatgc atattcttct aaatatgtag atgtagttaa aaactgcgct
    48841 aaagaatgca atatagatgt caaagaaggc gtttatatgt tttttagtgg tccaaactat
    48901 gaaacaccag cagaagtgag aatggcacaa attttgggag ctgatgctgt aggtatgtct
    48961 actgttccag aagtcatagt agcttctcac tctaatatag gagttattgg tatatcttgt
    49021 ataacaaata tggctgctgg catcttaaat caaccactaa gtcatgagga agtaatagaa
    49081 actactcaaa aagttaaatc agaatttatg aatttagtta aaacaacggt taaaaattta
    49141 taagttaaag cttattatag attttttgag gaagtttata ataaaatagt taatgtttaa
    49201 tttgaaaaga attaataaaa agctgtcttg atatagaaag ttatttctaa taaaggacag
    49261 tttttttata atactacaac atttgaaagt gttttaaagt ttaatattta tttaaattat
    49321 tataggttag aatggagatt tcaattatga gaatgtatga tattataaaa aaaaagagag
    49381 ataacctaga gcttagtaaa aaagagataa actactttat agaaaactat tcaaagggag
    49441 atatacctga ctatcaagct tctgcactat taatgtcgat ttatttaaat aagatgaata
    49501 aacaggaaac tgtacatctt acagaagcaa tgatgaactc tggagacatg ataaacctat
    49561 ctgagataga tggaataaag gtagataagc atagtactgg tggtgttgga gataagacta
    49621 ccatagctct tatacctttg gttgcttcat gtggagcacc agttgcaaag atgtcaggta
    49681 gaggtcttgg tcatactggt ggtactattg ataaattgga gtctatacca ggtttttcga
    49741 cagaaatgga gataagtaaa tttattgatt ctgtaaataa gtctaaaata gcagtttgtg
    49801 gtcaaagtgc aaaggtagca attgcagata agaaaatata tgctcttaga gatgttacag
    49861 caactgttga taatatatca ttgatatcat caagtgtaat gtctaagaag ttagcttcag
    49921 gagctgatgc aattgtgcta gatgtaaaaa ctggtgatgg agcatttatg aagacattag
    49981 aagagtcttt tgagttagct aaatctatgg ttgatatagg tactggtatg aatagagata
    50041 caatagggat tgtaacagat atggatgaac ctttaggttt tgcagtggga aactctttag
    50101 aagttataga agcaattgag acattaaaag gaaatggacc taaagacctt gttgagctat
    50161 gcgaagtatt aggagcatat atgttggttt tatcaaaggt agcacatgat tttgaagatg
    50221 gtgttgaaaa aataagagaa tcaataaaat ctggtactgc tatacaaaaa ctcaaagagt
    50281 ttatagagaa tcaaggtggt aataaagatg tagtagatga ttattcttta ttcaaaaaat
    50341 caaagtataa atatccagtt aaatcaacta aatcaggtta tataagcaaa attaaagctg
    50401 aagatgttgg tgtatcggct atgattcttg gagcaggtag agaaactaaa gatgatattt
    50461 tagatttatc agcagggata attttagaaa agaaagtagg agattatgta aatgaaggtg
    50521 aagttttagc ttacatgtat tataatgatg aacaaaagct ttcattggca aagggaagat
    50581 ttattgattc ctactctatt gtagattcaa aaatagaaaa aaataaatta atatatggta
    50641 ttgtaactaa agaagagatt aaaaagtttt agaaaatttt agaaaagtga ttataaaagg
    50701 cctaacatgt caaaaataat gttaggtttt ttattttttg atagactata aattatacca
    50761 aaaatatata ttttaaaaca ttttttacaa tataaataag tttattctac aagatgattg
    50821 aaaattaaag tatctagttt tataatagaa aatggataaa tatacataag gagtagatta
    50881 tattatgaac tttaaagaaa atagattgaa aatagcagta ttaataattg ttattttgat
    50941 tatcttagca ggtatatttg tgtttataca aataggtcct tatgataaaa ataacaaaaa
    51001 agatgtaatt atcgacgttc caagtggagc atctgttgga aaaatatctg acatattata
    51061 tgaaaataaa ttaataaaga atgaactatt atttaaactt ttggtaaagg ttagcaataa
    51121 agctccgtct ataaaatcag gaacatactt acttaatcaa tcgtattcaa acaatgatat
    51181 aataagtctt ttagtttcag gtaaaatata tcaggatggt ataaaagtga ctattccaga
    51241 gggagctaca tctaaagaaa ttatagcaat gttagtaagt aaaaatttag gagataaagc
    51301 tacttttgaa aatttaataa aaaaacctca agaattttat gataaatttc catatttgaa
    51361 ggaagatgga ataacctctt tagaagggtt cttgtatcca gaaacttatt attttaattc
    51421 aaagaaacaa tcagaagaag atattttaag cgaaatgtta aaagtatttg acagtaaata
    51481 cacggacaag tttaaaaaga aacaaaaaga attaaatatg actcttcaag aagtaatgga
    51541 aatggcatca ataattgaga aagaagctgt tcttgacaaa gacagaccta taatagcaag
    51601 tgtgttttat aatagactta aggtaggtat gcctcttcaa tcagatgcta caatacaata
    51661 tatatttgaa gagagaaaga aaattgtaac atatgatgat ttaaaaattg attcaccata
    51721 taatagttat aaaaataagg gattacctcc tacaccaata tcaaatcctg gtataaaatc
    51781 tattgaagca gcattatatc cagagaaaac agactattta tattttgtag ctaagattga
    51841 tggtggaaat aattatagta caaactatca agaccattta aaatacgtta aagaatataa
    51901 agaagcaagg gataaacaat caaaagatac aaaagcaaca aataaagaaa atacaaaaaa
    51961 ataaaccatg attataaaaa gtctaacagg gtaagaataa tgttgggctt ttttattgaa
    52021 aattgttatt cttagattta taataaagaa agtgtaaata tatataacaa tatgaattta
    52081 aaaaataata aattaatata aaacttgaat atttattatt ttagggagtg tttttatgag
    52141 taatatagtt aatgatttgg ttgaagatta tataagagtg acattaaaag aaaaagaagg
    52201 atttttaaaa gatttagaaa tttatgcaga ggaaaatagt gttcctataa tacataaaga
    52261 agtttcagat ttattaaaag tcttgttaaa ggttcaaaaa ccaaagagaa ttttagaagt
    52321 aggatgtgca attggttatt cttctatttt ttttgcaact attataggaa aagatgcaga
    52381 tataataact gttgagagaa atgaaaaaat gatagaaaaa gcaaaagaaa acataaaatt
    52441 agctggattt gacaataaca taactatact agaaggagat gcagaagaaa aacttagtca
    52501 agtccaaggt gaatttgacc ttatatttat agatgcagcc aaaggtcaat ataagctatt
    52561 ttttgattta gttatagata aattaaaaga tgggggactt ttagtatctg ataatatttt
    52621 gtataaaggt atggttgctc atgatgattt tgttgtaaga agaaaaaaga ctatagttaa
    52681 aagaatgaga aattatttag attatatatg taattgtgac tatttaagca catcattggt
    52741 accaataggt gatggtgtag cactaagtta taaagaaagt aaaagaggtg atgtagatgg
    52801 aattaaataa agtagaactt ttagctccag ctggtgattt ggagagattg aaaatagcta
    52861 taacatatgg agcagatgcc gtttatatag gtggagaaat ttttggaatg aggtctgcag
    52921 cgaagaattt tagtaaggaa gatatggcag agggagtagc ttttgcacat gaaagaggca
    52981 agaaagtatt cgtaacagta aatataatac ctcataatga agattttcta caattagaag
    53041 actatttgtt ggaactagaa gagataggaa tagatgcagt tataatagct gacccaggag
    53101 tgcttagtgt cattaaaaaa gtaattccga atatggaaat acatttaagt acacaagcaa
    53161 atacaaccaa ttatctaagt gcaaacttct ggtacgagca tggaataaaa agagttgtag
    53221 tggcaagaga attatctttt gatgaaatat ctgagataag agcaaaaaca cctttagata
    53281 tggatataga agcgtttatg catggtgcta tgtgtatttc ttattctggc agatgtttaa
    53341 taagcaacta tatgactgga agagatgcaa acaaaggttc ttgtgctcaa tcttgtagat
    53401 ggcaatatca tttggtggaa gaaaaaagac ctggtgagta ttttccaata tacgaagatg
    53461 aaaggggtac attcttcttt aactcaaaag atttatgtat gatagagtat ataccagagt
    53521 taatcaaatc tggtataaca agtttaaaaa tagaaggtag aatgaagact gcatactatg
    53581 tagctactgt tgtaagagca tacagaatgg caatagatga attttacaga gacccagaaa
    53641 actggaagtt caatccaatg tggatggaag aactaaaaaa aggtagtcat agacatttta
    53701 catcaggttt ttacttgaat aaaccaacaa cagaagacca aaattatcaa tcagcatcat
    53761 atgttagaaa ctatgatttt ataggtatag ttagagaaac agaagatgaa gatggtctta
    53821 ttgttgttga gcaaagaaat aaaatgtgtg ttggtgatga aatagaagtt atgggaccat
    53881 ataaagaaac tatgtttact aaaattgaag aaatgtacaa tgaagaagga gaagctatag
    53941 aatcagctcc tcatcctaga caaatagtga agttaaagct atcagtaaaa gttggtaaag
    54001 attatatgct tagaaaagtt atagaagaaa aggttgaaga ataataaaaa attgataaaa
    54061 atagtattag ccccttagaa attgtcaata atgaaattat ctaaggggtg atttcatgtc
    54121 aaaaaagaag actccatttt tgaaaaaagt gggaaagaga agctggtgta tatttactat
    54181 aattttaata atttatagtg ttttaattta taggctagtt gatatacaag tattaaaagg
    54241 tgataaatac aaacaaagtg ttgaatcaca aagcgttgag aaagtcgagt taaatagtgg
    54301 aagaggaata atctatgata gaaacaacaa gaagcttaca gatacaagta aatctcaggt
    54361 attaattgtt gaaaaagaaa aattaaataa taactataaa attttggagc ttattaagaa
    54421 agctactaag atgaatgatt tagatatata taaagctgta caagagcaat tgacacgccc
    54481 tataatacag attcagacta aaaatatcga taagagtatg aaaaaagaac ttgagaaaaa
    54541 tggaataatg gtagaagaaa aaactatgag gtacgcaaaa gatggtttat tatcacatac
    54601 aataggatat ataaaagaag atgataaatc aggtcaatca ggaattgaaa agagtatgga
    54661 tagtgtactt agaaattcaa atgaaaagta tataagtgct tttaaagctg gagatgcagg
    54721 aaatgaaaaa tctctaaaca tactaaaggg aagtgtaaaa actgttgata ataaggataa
    54781 agatagacat ctaaaaacta ctattgatta taacattcaa aaaaaattag aacaaatact
    54841 aaataaagaa gaaaatccta cagcagcaat aatctcggaa gcatctacag gtgaaattct
    54901 tgcaatgtgc tctagaccta attttgacca aaatgacata tcaaaaagtt taaaaggaaa
    54961 aaatggagaa tttgaaaata gagttataaa agcgacatat ccaccagggt ctgtatttaa
    55021 gatggtagtt ttattttctg cacttgaaaa tggtgttata gatgagaact atacttacaa
    55081 ctgtacaggt aagactaaag ttgggaatac taatgaaata ctgcgatgta ataaaagaga
    55141 tggtcatggt ttccaaaact taagacaagc tttttctaat tcatgcaatc ctgcattttt
    55201 agatatagct atgaaacttg gaaaagaaaa gatactcaaa tctgctgaaa aattacattt
    55261 atttgaaaag gttgatattg gtcttgatga agaaaaaatt agagaagctc caaaaaacat
    55321 atcaataaga aatttagcta taggtcaaga aaatatagaa tttacaccac ttcaaataaa
    55381 tcaaatgact caaataatag ctaataatgg aacattcaaa ccactatact tatataaaag
    55441 tcttgtggac aataatatga atacaataaa aacttataag tcttcaaaaa aagaagaact
    55501 tatttctcct tatgtgtgta cacaagttaa agaatatatg aagtctgtat ctaggattgg
    55561 tacagctaaa gacctaaaag atattgaagg tggttgtgga gttaaaactg gtacagctca
    55621 aagtagttta aataaaaaag ctatagacca tggatggata acaggatttt atcctgaaga
    55681 aagacctaag tatgtaataa ctgtgttagt agaaggaact caaaaaggaa ataaatctgc
    55741 aacacctata tttaaggaaa tatgtgaaag tataaaataa attatgtact ataaaattta
    55801 aggatatatc gtaactttga ttttagatag aaaattttgt ctaatgatgt acgatatatc
    55861 cttttaaaat atagtattaa ctttcttgaa caagctcttt ttctagtttt ttaagagctt
    55921 ttttttcgat tctagataca tatgaccttg atatatcgag tttttgtgca atttctcttt
    55981 gtgtcttata gccataagga gttaacccat atcttaactg gacaatttct ctttctctag
    56041 gagtaagaat tttgtttagt tgttcgtaaa gttttccaac ttgaactttt agttcaactt
    56101 catcaagaac atagttagcc tctgtaccaa gtatatctaa aagacttatt tcattacaca
    56161 ctactcttta tatgttattg gtattactgg tcttcatgta atttcaaatg gaccattaat
    56221 tcaaaagaat ctagtttttg ttctttagac ttataataaa ctgctttatc aattacagat
    56281 tttaggagtt tattttttcc aagggaatct gaatcatggt aaagtgataa cacagtttta
    56341 atatcttcta ttattttaga attgtctttt ttaacagtac ttaatctatt ttttactgtc
    56401 ttaattgaat ctttaatttc ttcagttctt gaacttatat tgttggacct ttcaataaat
    56461 gtatcttcat catatattcc tcgttctagt aaatcatata acttattttt ttgcttgcct
    56521 aattctttta attcagtttc taagcccttt aagactcgtt tttgaaattc tatattacta
    56581 tctttattat ttttatctaa gtctatatca gatgctttta attgatattt ttttacagtg
    56641 tcttctaaag attttaaaat ggcagcttca ataaattcaa atcgagaact tttattgcaa
    56701 ccaggatgat aacaaattat atagtcgtgg tctgcatatg gtctatatac taatggtcta
    56761 ccacaatttt tgcaaatcac tattcctgca agtggatttt ttatttgatt accatagctg
    56821 acatgaccat tttttttgag tatattttga gcttttttcc atgttatttc acttattaaa
    56881 ggttcatgct ttccttttgc ctcaatccat tcatctttag gtcttagttt aactgttttt
    56941 attttatttg gatttttaga ttttttgtag tccttttttt gccactgaat gtagccacaa
    57001 tataccttat tttttattat atttgtaatt gcagaattgt accaattagc acctgtagca
    57061 gttttaatgc ctaagttatt taggtatttt gatattttac tacaacccat atcttcatta
    57121 atatataagt caaagatagt cctaactaca tcagcatcct tatttggtac aagtattctt
    57181 tcttttctac cagttgttac agcatcatat ccaaatggag cagaggttcc tatgaaattc
    57241 ccttcttcaa ctgattttat tctacctctt tgcatccttc ttgatataag ttttaattct
    57301 tttcttgcca taaatgcttc aaattctgaa tactcctcat caaattcatc tgttaggtcg
    57361 tatgtttttc taggtgtgat aattttggtc ttactttctt tgaatgtttc taatatgata
    57421 ccttggtctt tcatgtctcc acgaccaagt ctgtctaagt ccattactaa gactgcatca
    57481 tatttatttt cttcaacttc ttttagaagt tcaagcattt tgggtctatg tattatactt
    57541 tcaccagaaa ctaattcttc ttttatctca attacatcta agttttgttc ttttgctagt
    57601 tttagtaggg ttgatttatg tctacttaaa gtttcaaatt cgccttgttt ttctgcctct
    57661 tcatcagcac gggattttct taagtatatg gcaacactca taataagtaa tcacactcct
    57721 tatgcactat tatatagtaa atcaatagag tttaaaatat agaaaataag catgtcaaaa
    57781 gttaatttaa ctttcaacat acttattttc tataatatta atgccttata atcctttaat
    57841 atcatttaga ttagtttttg catattcatt atccgggtta agttcaagcg ccttattgta
    57901 agattctcta gctttattaa attctgattt actcataaaa ataacacctc ttaaattgta
    57961 agcatcacta gcgtctattt tataagcttc attattttta tctagatatt ctaagtattt
    58021 atcaattgac tttaaagcct catcatatcg ttgtaactct atataaatat atgattgtgt
    58081 ataatatgca agtggatttt tatcatcaat ttctatagct tttttgatat caatagcagc
    58141 tttattataa tccttatctt catctttttc tgtttctgac ctagcgattt tgacataatt
    58201 agagctaaga gctcttttaa agtctttatg ccataataat ttatattcat tatcttcgat
    58261 tataaaatat ctatcaaaat ttgcagtatc ttctttattt tcagtgatat actttatagt
    58321 atcagtttct tttattttaa caagatttgt gtactcttta ttagaatatt tgtatttctc
    58381 tactttattt tcttcaccaa gtttataatc ttttagggtt tgaagtttaa ataaagtatc
    58441 ttgatattct tcaaattttt ctagactgac gctttcctta ctgtctttac ttaataaatt
    58501 atatgctact tcgtagtctt tgatttttat actatcaagg tactttttag ctacttctgt
    58561 agcactttct tttggagcac atccaaccat tgatatcata attaaaaatg atactaatat
    58621 tgctgttatt tttttactga ttatcataat ataatccccc ttatattgtc tctcattaat
    58681 tttattttat caccatattc taccaaagtc attaatttag gagtgatatt tttcattaat
    58741 attatattaa gtaataataa ctttttaaat ttatggtaac attttcctga aaagtattat
    58801 ataatttagg tgaaaatatt taagagaagg agatattatg aaaattaaaa aattattaac
    58861 aatagggtta tctttatcta tatttattgc tagttgccct atatctgcaa atgctcttga
    58921 taaaattgaa tcaattaaag gtgcagataa atacgaaact gctggtataa ttgcagacaa
    58981 acaaaactat actacagcta tattaattaa tgcagacagt acaatgtctg atggattaag
    59041 tgcaagtgga cttgcaggag ctataaacgc tccaattctt ttgactaaga aaaataacat
    59101 accaaatgct acacttaaaa gattagaaaa ggcaaaaaaa gtatatataa ttggaggaga
    59161 aaactctatt gataaatata ctgaaactgt tctaaaaggc aaaggaatag agattaaaag
    59221 acttcaaggt agtgatagaa ttaagacaag ctataatgtc gcaaaagaaa taaactcaat
    59281 taacaaagta aataaagtca tattgactaa tgcatttaaa ggagagcctg atgcaatgag
    59341 tgttgcacct gtagcagtta gagataaagc agctatagta ttaactgatg gaaaaagtgt
    59401 tccatttaat acaactggtg tagaaagtta tgtaataggt ggtacttcat caatgaatga
    59461 caaaatagtt aaggatacta attcaactag attaggtggg acagatagat atgatactaa
    59521 caaaaagata ataaataaat tttatagtgg agtaaaagaa ttttatatag caagtggtac
    59581 tgatttagtg tatgctcttg ttggttctac tgctgctaaa aataatgcta ttgtgttagt
    59641 tgataatgat agtaataaaa gtgtattgaa aagtacaact aaattaactg ctataggaaa
    59701 tttgagtgat agtatattac agcagtgttt aaatgtaaca aaaaatatag gcgattctaa
    59761 tactggaaga gaatatacag tagaagatgc tgctaatgca gtgaaaaaac tattaggtgt
    59821 gaagaaattt tattacaaac ctaattattc agaagatgaa gatttaatat ttctactaga
    59881 taaaaatttt gatttttcag gaaaagagta ctatgtattt gcttacatta aacagggtgc
    59941 agagggtgat gttagatact gtgtagctaa aaatgatatg agcaaagttt atacatatga
    60001 tgttaatggt aatatgaaag tatgtaatcc agatgattat aaaaataaag gaagtataac
    60061 agaggaacaa gcaaaacaga tagcattgaa agaatgtgct aagagacata aagtcagtat
    60121 caataactta gtagttgata tgatagattt tagagatgat atatatagtg gaaatgtcta
    60181 tgctgttaaa atcaattcaa aaaatagtaa tggtactttt ggttggtttt ttatagatat
    60241 agaaactggg aaagtttata attaaaatta gaacttttaa acaagtgttt ttaattatat
    60301 aatttaccat aatatgttat aatattttta gcaagaagat ttaacttaca atctatagag
    60361 tggagttcat gtacaaaaga ttatcctccc aacgattcga agggaggtga gttcatggat
    60421 aactttttgt ttaatgtttt agctagttta acagctagtg tggtagttta cttaatcagt
    60481 aaactattca aaaaagcaaa aagccactct cgcacaaaga gtgacttacg agttgaattt
    60541 aaatttatat ttaaatccaa aaaataaact ctgtttatga tgaactccac tctaacgcaa
    60601 aatagattgt agttcttctt gcttttatta taccacaaaa cattataaat aaaactgttg
    60661 tttataaaaa aatgcaaagt aaaatttttt ataaacaatg ataaaaattt tattatagag
    60721 ctattaactt attaaatatc tgtaaatgag cagatatttc ataagttaat atttattgtt
    60781 tacataaaat atttggtaaa aataagtaag ttttatatgt tataatagtt gtagcaagaa
    60841 taataatcga aagcagcaag catgtcagct ggtgttatat cagtttatat ttatgataaa
    60901 ataaaaatct cccaaacgcc aataagaatg attcaaaaaa ataatatttt cacttaaaaa
    60961 taatcactct ttataggagt aaattatttt cttgatttta ttatactaca aattggtaca
    61021 gatatttaaa aataatatat tcataatatt ataaatagtt ttgcagtgag cgatattttt
    61081 gataaaatag ggcttaacag ttgaaacata aggcattgag ggtatatgat aaatattatc
    61141 atttgcacta ctcgtggttc actgcaaatt tgagagaatt gtatagatgt aagtcttgga
    61201 aatgctcaat ttattttggg gttttatatt aactaagtgg tatgtaaagt gttcccctct
    61261 aagctctgta tatatatatt ccgtttgttt tatattaact aagtggtatg taaagaagtg
    61321 ttgatattta tattgtctat ccccgctagc ttgttttata ttaactaagt ggtatgtaaa
    61381 gtcgtaaatt acgaccgtac ctttcgtaaa ataacaatcg ttttatatta actaagtggt
    61441 atgtaaagct ttttgagtat gagtacaagc ataggaaaat taatagtttt atattaacta
    61501 agtggtatgt aaagaacaaa aaagatacat ctgtaactaa tttttatact gttttatatc
    61561 aattaagtgg tatgtaaaga cagatataat atcaaaataa ggttaggact tttaaaagca
    61621 tagccatatc agtaaaaatt taattatcat ttaaaaaata aatttttatt taaagaatac
    61681 acataaatat ttccagactc aactctctta aactattttc atatctttta gtagatacct
    61741 ttgttgtttt ttaatcttta gcaacttgtt cttgagtgaa cctcttattt tttctaagtt
    61801 atttcaagct tttcaaaaaa ttctctattt atattcatca cacatataat acaattaatt
    61861 cagctttaat tgccaatatt tagtttttct gtatctgata aaccaagaat ataatcagta
    61921 tataaaccaa aaattttagc aaatattatt aacccatcgt ctcttgttgg tctttcacca
    61981 gactctattc tattcataac acttgtattt atatttgttt tttcaagcaa ttctttttga
    62041 gaactattca tattttccct aatatattta atcctttgat ttacatccat acataattat
    62101 ctcgaaaagt ttctgttttg gaaatattgt tattgaaata tagcaattaa tgtgaatata
    62161 tgaaaaagat acactatgat tttataaaaa agaagacata cataaaattt atacttacaa
    62221 agtgttgcaa atgagttagg ttttaaaaat gcatcaattt attttaagta caggaaaggg
    62281 gaatattcaa ttagagttga tatgttacct ataaactatc taagatactt gattatgata
    62341 tagaaaattt ttttacaaat taaatttcta agatagaaat ttaattaaga ggatgatgag
    62401 gatgaataat ttacaagtaa tataaagaaa taataagaga attttaaatg catagcaact
    62461 agcagatata tatgaaaatg atgtaaacaa tatataaact aacttcaata gaaataaaga
    62521 gaatgtaaat tatgttttat tacaattcga gtatttgaaa gaatttaaga actaatctat
    62581 taatagtcag ttgatttcaa aacattctag tcaattatac ctttggatct aaagatgtgc
    62641 aaatagacac tgcaaaatat tagatattga taaaacttgg gaacaatttg acaatttaga
    62701 gaaaatatat ttcaagatta agcgagagaa actaagctgt atagaagatg tattaataga
    62761 aaatttaaga tttaaggata caaattaata aagcaataat atggcattag atgtaaaaat
    62821 agaagttaaa ataataaaag atgttgttat attaaatata acacattgga aaaaagaaac
    62881 aagtctatta acagttaaaa tagctagaag tcaaggtgat ttaagttatg taaaatattt
    62941 tgctaatgag agctaagagc ttcttaacaa gagttttggt gtgtctcttg aaacaaggtt
    63001 aacaattaat aaaagacgaa tggcagaaga aggcgtatgt aagtttaaaa gagataagtt
    63061 aaacaaacta gatactatgt aagaggataa gaaacttata gaagaatatg ttacttttgt
    63121 aaaggattta gctttaaaat atagaatata aagtgatttt aagtaaaaat tgtaacaatt
    63181 aaataagtat aagacaagtt tatgagttca tattttataa caaggggtga tgttatgaaa
    63241 atactacaac aatttaaacc atacaaatat gataaagaaa aaaatataga atatgatgaa
    63301 aaaattatag ctaatcaaaa cacagaagtt tatatagtat atccacgtat atctgatgaa
    63361 gaaaatagga gaagatggga agagttcaat gaagtagcta aagaagtagc tattaatata
    63421 gctatgaaga aagttgagac agtagaaagt taagagaata gagaagttgc taatacttag
    63481 cactttctca tagaagaaaa ttggacaact atttttaaat aattaggaat aaaaaagtta
    63541 aatcatattt gaaaaaaggc gataaaattg ttgagtattg ttattagtaa taagaatata
    63601 ttaactatgt attaaaagaa attataagtt tttagtaaat atagtattta aggatattaa
    63661 aactggtaaa aaatatcatt tagactatta aataaaaaag tgctggtcaa agtagcaaca
    63721 caataaataa aaaaacttat agaaattata agataaaaag atgagatttg aaatggtcga
    63781 agttaatata aaaattaaag gtgaagtaag tgaaataatt aactcaatta gaggtatatt
    63841 taatagcttt gatgaagaat taatattaga tataaaatca gagtaagaat ataaaggtag
    63901 atacgtagaa agttataagt aattcaagac ttaaattaat ttctagtttt tttaatgcaa
    63961 aaatttaata aatttcaaaa tataataatt ctgacatcta aaaagagcta ttaaaaatga
    64021 ataatattga ctattaagat atgaatagtg tacatatatt aaattaagat atcaagtagg
    64081 ttaattttat ataatgatgg ggctcaaatt gaagaggcga tataaaaatg tattaatata
    64141 tataaatata agaactataa tacatattaa ctgtaaaatt aagtttatag agttatattt
    64201 aacatttaat gaactgaaaa ataaattagc aaataatgaa aaaacaacag taataaatga
    64261 gaataacaaa gtattttcag gattagtgga tttttctaat tgagctttaa tcctagtttg
    64321 ttcaatttcg atatttctaa tattatcaat ttatttgtaa taattatcaa tttttgcgaa
    64381 atatttttga taatttatag aactttatat attttccttg aagtattttt cgcccttatt
    64441 gtttggtttc caattttaaa actcataaac tcccaataaa ttcatgtata atatacctta
    64501 atttataata tatttaagag tttatttcag taatttttcg aacgattttg atatatttca
    64561 tttatagttt tcaaaaactt atacaaaaaa gagtgtttca tctttctaaa cactcttttt
    64621 ataagaattt gaataataat tttttaaaaa ttgataatat ggttatttct ttttcaagtt
    64681 tatagtttcc tcatttccat tccaagatat aattagcttg taactagatt ctctatttga
    64741 ataaagacct ggtctttgta tttttattgc tttttcttga cctgcttcta aaatttcatt
    64801 ttttacaact acaactgcat cttcaccatc tataaattga taggaataat taaatttttg
    64861 ttcaactaca tctttactat ttttatattt tatttctaat tttgagtgtc cttcagtagt
    64921 tacaaattta gctttccaat tttctgattc tccaataaag acatttttat cattttttat
    64981 attaagtata gctatcacag agattattaa aactataaga atacctataa aaaatttttt
    65041 cctcatatca atccctcctt tgaataatta taacatgaga atagttattc aatactaatt
    65101 ttaaaaatat tgtggattta tattctatta caatagaatg tctgttataa caaataaaaa
    65161 gtaaggatta atatgaatta taggttaaaa aaatctgtaa caactatttt atctttactc
    65221 ataatgacaa tactattacc aacaaatgca agtgctagta aggtagaaga ttcatctatg
    65281 aaagtaatgt attcagagaa acaagtaaca gacatggaca aattatacga tatggctaaa
    65341 aatggaatta ctgatgttaa aagttccaat gaaaaaggta taatcaaaaa tgcaagaaca
    65401 ggagagtcca tatacgttga ttctatatca acaactcaat tattagaagt taaacaatct
    65461 aaaaatttaa catcaaagat atattctaag acagtttttt tgatactaat acaataaagc
    65521 ctcttagaaa agcaagtata tcagaagatg gatgggataa aactggtggt gtaaaagcat
    65581 ctggtacagt tacatataca atatcaaaag gagatggagc atttcaatat atttccgtta
    65641 gtaaagtttc tggaaactgg tctattgcag acggccagat tcgtgtaaca aatagaaggg
    65701 tgcatataaa tcaaactgga tttatttcta atgagctagg aattaagcag caacaaaaga
    65761 aagagccaac atcaaattct ttctcatata atgcaccttc tagttggaaa ccagtaggta
    65821 gagattcagt tcaatacaat tgtgggtttg aattaattgc aacattagta agaggaggaa
    65881 gttcttgggc attcaatttc attgttcaac cttaaaatat tttaagtgaa ttttattaaa
    65941 atcaaagata tacatatact ttaaaattag agaatgtaaa ttaatttgtg tttttaaaga
    66001 taattcctat gttatagtaa caattgctta tttactatta atatttctta atatatgaat
    66061 tttattttaa taaattataa aaaatgtata ttgggatgtt tttaaatatt tttggaaatt
    66121 atatatatta taacaattat agcaaatttt tagggtataa tttttaaaat tatattctac
    66181 tatcaaaaga tatgaataaa tgattgtata tattatatca atttttatat ttcatagtcc
    66241 attttttcat cacattttcg cttgtcaaga ataatatctt taagagtgaa gcgtttgatg
    66301 taaatctgga ctttgttttt gagacatttt tgaaatattt attaaatctt tcgattatac
    66361 ttgtaatata tattatagat attacttatt ttagtaaaac taaaggatga tgctacaaca
    66421 ttccagttat ttcccaaaat tttaatttct gaagtatact gtttacatta tttttaatct
    66481 attttatcta aagtcgataa ggcttcttat tcattaactg acctatatac agatttaaaa
    66541 ttacttatta attcttttga gtccttgtat gaaacatatt taaagtattt cttatctaat
    66601 gtattaaaca tttttgtatt acagaatgag gataacttgt agctattgtc tctttgaaat
    66661 aagtcatctg tgtttactcc taaaactact tatatctgta gattttataa gttctccatc
    66721 ttatataatt tctttgatta attgttttct taaattattc ataaaaaaat ctccgttaca
    66781 atatttgatt atatttaaat cattgctata aagaagactt ttattaactt tgaaaaacaa
    66841 attattttaa ttgctggtga ttagttgcta ccttcttttg atattaattc tattagattt
    66901 tatatagtta tatatctctt tctaactaag taatataata ttactgttat agtagctact
    66961 aatattattg ctattattgt aatatctaat ctttttgtaa ataatcctat tgctatatat
    67021 tcaaaacctg ttacatatcg ataaagtact tctagtttaa tagttctttt tctatctata
    67081 tttccatatt ttgattctaa tttattaaaa tatttttcat tacaaaaacc aattttaatt
    67141 tcaattaaag atattattgt agttaatatt ataattccca atcccataag tataagataa
    67201 atgttcctaa aattcataga agatacctcc tttataatat aatcatcaac tcatgttgta
    67261 ttaattttcc taatcaaagt ataacagtaa cacaagtata aagagtatct aaaatattac
    67321 aaatattaaa tgagaaaaag atggtaagtt aaccccacca tcttattttt tatctactaa
    67381 tgaatgatgc tacaaaagtt agtataaaaa atgaaacaaa caatataatg aacttgtgta
    67441 atttacttag ttggaatgct ttattacaaa ataataaaat taatgctgaa acaattgcaa
    67501 aaagtagata ttttataatc attttatcac ctattgattt ctacagattg taagtagtat
    67561 ttggttacac aagcacctcc tttattataa cgtatttttt aaatttaaag atttttccag
    67621 atgttatatt tgactcaagg tacattgtaa ttaatctaac tataagtggc aagtactgtc
    67681 ctataccagg aatagcacca gccaaaagtc ctgaaatccc tataccagca tcagttaaag
    67741 ttaatgcaac tcctttagaa agatataaat taaaacctat tctagcccaa actattttat
    67801 ttatttcagc ttttctataa tataattcaa gtagtgtcaa tatcgttctt ctta
//
LOCUS       DAGSRM010000010        67585 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.010, whole genome shotgun sequence.
ACCESSION   DAGSRM010000010 DAGSRM010000000
VERSION     DAGSRM010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 67585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67585
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.010"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..160)
                     /locus_tag="MJE33_000731"
     CDS             complement(<1..160)
                     /locus_tag="MJE33_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBY9646699.1"
                     /translation="MNREKLVAFIRYLSLFILVVSYLKNVENTLYPVITMIILFLIII
                     NNQVRFFSL"
     gene            344..688
                     /locus_tag="MJE33_000732"
     CDS             344..688
                     /locus_tag="MJE33_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HBY9646700.1"
                     /translation="MNNKEKGDFGEKVAVNYLLSKGAKILEKNYRLKIGEIDIIAKME
                     DEIIFVEVKSRSNIKFGYPCESVSFKKRKKIIGVASYYIIKNNLNNTPIRFDVIEVYL
                     LEKRINHIMNAF"
     gene            719..2320
                     /locus_tag="MJE33_000733"
     CDS             719..2320
                     /locus_tag="MJE33_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HBY9646701.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDVSNGIPSFNIVGLPGKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQNIKRIFIPIENIKESLFVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVRIMSFLDDKSRKENEELEKDNSYIDCKYTKINNEESKYDEDF
                     KDVKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISVEEMIEVSKV
                     YSILGMINETKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLVAAMNPCPCGYYMSDTECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     KKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFNKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFK"
     gene            2339..3439
                     /gene="dprA"
                     /locus_tag="MJE33_000734"
     CDS             2339..3439
                     /gene="dprA"
                     /locus_tag="MJE33_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBY9646702.1"
                     /translation="MEKRDTYLWLKSIGGITTKTIEIIENEIVNIEDIFDFSEKEIYN
                     LKNISLNIRKNIVKYRGHAYLENIKELLYKKAIKYICKYDKEYPENLKNIYNAPKLLF
                     YKGDIGLVNNNFNIAIVGSRKPTAYGINCAKTISCQLSQYGVNIVSGLAIGIDAYSHI
                     GCMSGKSKTIAVLGSGVDNPLPKQNLHLSNKILENGGLLLSEYNINSTVAPYHFSNRN
                     RIISGLSDGVVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQMSRGCHKIIKEGA
                     KLIENIDDILNEYNIFNIIDKKINQKYDNISLNAKSKQIIEAIKREGNLHIDSICDYT
                     GIEIKYVNSIINELVLNELVVEMNNKTYSLNV"
     gene            3472..5559
                     /gene="topA"
                     /locus_tag="MJE33_000735"
     CDS             3472..5559
                     /gene="topA"
                     /locus_tag="MJE33_000735"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBY9646703.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELENEEAVNQILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVNNLGENYYKNTGD
                     KKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            5724..6509
                     /gene="codY"
                     /locus_tag="MJE33_000736"
     CDS             5724..6509
                     /gene="codY"
                     /locus_tag="MJE33_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBY9646704.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDVQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            6616..7881
                     /locus_tag="MJE33_000737"
     CDS             6616..7881
                     /locus_tag="MJE33_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBY9646705.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKSFYKINATNSSLEDIKKVISELGSINNVNGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKLDIEKGL
                     KRAVEVLNEDSYMDIECNNNAIEDIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDVIRASTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDAHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSIYYIPQENKTEKNIKKYLEYLNSDF"
     gene            7912..8682
                     /locus_tag="MJE33_000738"
     CDS             7912..8682
                     /locus_tag="MJE33_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY9646706.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADVGEHGEIKAILLKYFEFMMFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDALAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNAKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRAKGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            8791..9237
                     /locus_tag="MJE33_000739"
     CDS             8791..9237
                     /locus_tag="MJE33_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBY9646707.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            9255..10448
                     /gene="nifS"
                     /locus_tag="MJE33_000740"
     CDS             9255..10448
                     /gene="nifS"
                     /locus_tag="MJE33_000740"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HBY9646708.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGVAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGKVDLKQLEDSIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLENRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     QGLVK"
     gene            10502..10942
                     /gene="nifU"
                     /locus_tag="MJE33_000741"
     CDS             10502..10942
                     /gene="nifU"
                     /locus_tag="MJE33_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HBY9646709.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGKTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAQKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            11231..12310
                     /gene="mnmA"
                     /locus_tag="MJE33_000742"
     CDS             11231..12310
                     /gene="mnmA"
                     /locus_tag="MJE33_000742"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBY9646710.1"
                     /translation="MNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDVVE
                     IEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCIAC
                     NKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYNLT
                     QEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSKKR
                     IEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDNED
                     LFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGEDTIKIVFDEAQRA
                     ITKGQSVVMYDGDIVVGGGIIEKSL"
     misc_binding    12453..12691
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            12759..15398
                     /gene="alaS"
                     /locus_tag="MJE33_000743"
     CDS             12759..15398
                     /gene="alaS"
                     /locus_tag="MJE33_000743"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBY9646711.1"
                     /translation="MEKMGLNEIRSKFLEFFESKGHYVANSYSLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVRLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDRESWKEDPLSK
                     LESTVDSTFNGYSEIYGEGTIEAIVKDDELVQSAEEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGRISNGDKVKTLVDRETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     gene            15423..15680
                     /locus_tag="MJE33_000744"
     CDS             15423..15680
                     /locus_tag="MJE33_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBY9646712.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            15690..17861
                     /locus_tag="MJE33_000745"
     CDS             15690..17861
                     /locus_tag="MJE33_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646713.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSYASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGQKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVKNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYDTMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILGTFGLE
                     NKEMVGRSYTLVNKEDNISYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKDNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLLVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAVLLAVSAVVVVFAFAALDILSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIISINMIIFEKNN"
     gene            18067..18492
                     /gene="ruvX"
                     /locus_tag="MJE33_000746"
     CDS             18067..18492
                     /gene="ruvX"
                     /locus_tag="MJE33_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBY9646714.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKAETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            18570..18857
                     /locus_tag="MJE33_000747"
     CDS             18570..18857
                     /locus_tag="MJE33_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBY9646715.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
     gene            19139..19597
                     /locus_tag="MJE33_000748"
     CDS             19139..19597
                     /locus_tag="MJE33_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBY9646716.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(19714..20451)
                     /locus_tag="MJE33_000749"
     CDS             complement(19714..20451)
                     /locus_tag="MJE33_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBY9646717.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFVAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTIGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKL"
     gene            20668..22344
                     /locus_tag="MJE33_000750"
     CDS             20668..22344
                     /locus_tag="MJE33_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBY9646718.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
     gene            22550..22750
                     /locus_tag="MJE33_000751"
     CDS             22550..22750
                     /locus_tag="MJE33_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protein"
                     /protein_id="HBY9646719.1"
                     /translation="MDDVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            22804..23967
                     /locus_tag="MJE33_000752"
     CDS             22804..23967
                     /locus_tag="MJE33_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBY9646720.1"
                     /translation="MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLN
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI"
     gene            complement(24178..24600)
                     /locus_tag="MJE33_000753"
     CDS             complement(24178..24600)
                     /locus_tag="MJE33_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HBY9646721.1"
                     /translation="METKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFNDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
     gene            complement(24621..25457)
                     /locus_tag="MJE33_000754"
     CDS             complement(24621..25457)
                     /locus_tag="MJE33_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBY9646722.1"
                     /translation="MRQEERTVCFDRELKIEAYRFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPKFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLQ
                     SNLENTNIEIQAVCDYLENNYAEHIVLDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPIDAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
     gene            25755..26351
                     /locus_tag="MJE33_000755"
     CDS             25755..26351
                     /locus_tag="MJE33_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HBY9646723.1"
                     /translation="MLSMNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGR
                     MTLNPVRHVDPIGLLAMFILKIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAI
                     LCVIINKYVHMYAINTIAGYVLLYSMGFAAFNLLPIPPLDGWGVISTFVPYKWNEYLY
                     KFESMSYIILLIALFTGMYRIILSPIYTVLLKIVYLFV"
     gene            26381..27100
                     /locus_tag="MJE33_000756"
     CDS             26381..27100
                     /locus_tag="MJE33_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBY9646724.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDEEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDISIEAIKNILPYIFKVKTSQIENANDE
                     KLDKIVRKKIISVEEKILYIRDIIKDKIEVTFTNIIKSYENDEIIATFLSILELIKEK
                     EIVVVQDIFFDDILIRKSSEC"
     gene            27102..27641
                     /gene="scpB"
                     /locus_tag="MJE33_000757"
     CDS             27102..27641
                     /gene="scpB"
                     /locus_tag="MJE33_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBY9646725.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIINKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENELIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVATNE"
     gene            27803..28432
                     /locus_tag="MJE33_000758"
     CDS             27803..28432
                     /locus_tag="MJE33_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HBY9646726.1"
                     /translation="MELNELFNFKYLIIAVIFIISVTMLILISHINILIIADIKNKDI
                     CLKLNIKYMFNLININRQLYPAENSKNNDKKEGMKNNIDSSILLADDLLSIYRLLKKI
                     KIHELYSNINFGTGNIGLTSSVYVLINTLYGNLFNMIDAEKMYLNVNPDFTKDYVLGN
                     IRIHIRPRIKALFNIIIMINKIMNKNKGNKEGDSNESNRFDTESYGNNS"
     gene            28386..28814
                     /gene="ytfJ"
                     /locus_tag="MJE33_000759"
     CDS             28386..28814
                     /gene="ytfJ"
                     /locus_tag="MJE33_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HBY9646727.1"
                     /translation="MRATGSIQSLMETTLETIKGSIDANTIIGDPIKTDTTVVVPISK
                     VTIGFGIGGGEYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETR
                     IMSLDSNINLVDNILSITPRVLEKIQNISQSNKNQDKNNM"
     gene            29062..29502
                     /locus_tag="MJE33_000760"
     CDS             29062..29502
                     /locus_tag="MJE33_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBY9646728.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDIDNKDKLFTSLEESKNIIEQLF"
     gene            29518..30969
                     /locus_tag="MJE33_000761"
     CDS             29518..30969
                     /locus_tag="MJE33_000761"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HBY9646729.1"
                     /translation="MNIKYSKEILKSLENAITVVALFEGEDVFTTFDKINMISRNFNS
                     KENSIDTVTLIENNNLVNVCFVGFGKRELLNREKIRIIGGNLCKKLKNILKNEAYKSS
                     DVNVLNLNLNSDEIGCFVEGILLGNYKFDKYKTKSKDFERKEVDTITIFTEKEVECQI
                     EKAKILANSTIIARNLVNEPSNVIYPKTLAVETVKLGAEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQSKLKKNVIAVVAACENALSGGSYKPGDIISSMAKKTIEVLNT
                     DAEGRLTLADAIYYIINNEKVTKVVDVATLTGAALTLLGNVATPIVTNNDDFYCELEK
                     AATLSGERVWKMPIYDEFKDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGNTPWIHM
                     DIAGTSTSSKSIGYKAKGATGEPVRTLYYLAEI"
     gene            complement(31060..31728)
                     /locus_tag="MJE33_000762"
     CDS             complement(31060..31728)
                     /locus_tag="MJE33_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HBY9646730.1"
                     /translation="MFKIFLFSSEQFVSLFIFGLFLYYCPKLTKNILPYSYTVEKIIC
                     TLLVIIMALEQLLLISSGNYSTLNSLPIGINYICIYLCIAILIFKQYHLFNIFFSWSL
                     VCSVGELIFSKNLGYEFPSLIYFIFIFSKCLIIYADIYMVDVRKFRVNRYALRDNLAI
                     CFIYFSFIFLLNTFTNSRYYYGFLSHSTTAIFTFIFVTSIMYIPALLFNRDTFILEKK
                     KKSK"
     gene            31997..32137
                     /locus_tag="MJE33_000763"
     CDS             31997..32137
                     /locus_tag="MJE33_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646731.1"
                     /translation="MGIIESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERR
                     NE"
     gene            complement(32312..32632)
                     /locus_tag="MJE33_000764"
     CDS             complement(32312..32632)
                     /locus_tag="MJE33_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBY9646732.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NMDK"
     gene            complement(32752..33489)
                     /locus_tag="MJE33_000765"
     CDS             complement(32752..33489)
                     /locus_tag="MJE33_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBY9646733.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            33704..35527
                     /gene="acd"
                     /locus_tag="MJE33_000766"
     CDS             33704..35527
                     /gene="acd"
                     /locus_tag="MJE33_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HBY9646734.1"
                     /translation="MKKKAALATLAMLPLGVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVI
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISESNGWSKIKYNGKDAYVS
                     SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKINFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNSNGNNSSSSGQVNGM
                     SGISGAKIDYKSLSYTLESHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSS
                     FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRDGISESSLNSYLNGLSSSVFKNQ
                     GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG
                     IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD
                     YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            35742..40040
                     /locus_tag="MJE33_000767"
     CDS             35742..40040
                     /locus_tag="MJE33_000767"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBY9646735.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHSAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEVLPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA
                     VLRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG
                     GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            40244..40705
                     /locus_tag="MJE33_000768"
     CDS             40244..40705
                     /locus_tag="MJE33_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBY9646736.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIANGKEMEFNRKDVAIVRLAIKF"
     gene            40735..41889
                     /gene="nusA"
                     /locus_tag="MJE33_000769"
     CDS             40735..41889
                     /gene="nusA"
                     /locus_tag="MJE33_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBY9646737.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVRSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELDTSEDNFSEEE"
     gene            41896..42177
                     /locus_tag="MJE33_000770"
     CDS             41896..42177
                     /locus_tag="MJE33_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBY9646738.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK"
     gene            42167..42478
                     /locus_tag="MJE33_000771"
     CDS             42167..42478
                     /locus_tag="MJE33_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HBY9646739.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            42500..44440
                     /gene="infB"
                     /locus_tag="MJE33_000772"
     CDS             42500..44440
                     /gene="infB"
                     /locus_tag="MJE33_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBY9646740.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE
                     LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            44483..44863
                     /gene="rbfA"
                     /locus_tag="MJE33_000773"
     CDS             44483..44863
                     /gene="rbfA"
                     /locus_tag="MJE33_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBY9646741.1"
                     /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM
                     YMDSLIKRVNEKNAQQNEDENYDE"
     gene            44844..45827
                     /locus_tag="MJE33_000774"
     CDS             44844..45827
                     /locus_tag="MJE33_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HBY9646742.1"
                     /translation="MKIMMNNMDSIINYIYEGDYFIVTSHISPDGDNIGSTLSMYYTL
                     KNLGKNVYYVLDDEAPLNLRFLVEDVKIYKSNEFNMKNYSLIALDCGDKMRICVSDEI
                     KNNASKLICIDHHASNDSYGDLNYVDIDASSTCELVYNLLVRFQQIKDINIINEDIAT
                     CLYTGLVTDTGNFSYSNVHASSFEMAKNLLVLGAQKNTIIQNIYQSNSSGYYKLLGEA
                     LKGLEIFDSKVSSIVLTQDMMNRNNISFNDVDGITTYTRDIKGIEVGILFKEKKQNEI
                     KVSFRSKNYVDVSEIAKLFGGGGHVRAAGCTIRDSIDNAKKMVLEAVLKSI"
     gene            45840..46745
                     /gene="truB"
                     /locus_tag="MJE33_000775"
     CDS             45840..46745
                     /gene="truB"
                     /locus_tag="MJE33_000775"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBY9646743.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYSNGGSIDDKRFLKNNFDKDDEFVRVYST
                     DNFLGLGKLSKHNNTISVKSDKMFI"
     gene            46876..47805
                     /locus_tag="MJE33_000776"
     CDS             46876..47805
                     /locus_tag="MJE33_000776"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HBY9646744.1"
                     /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEHAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            47909..48166
                     /gene="rpsO"
                     /locus_tag="MJE33_000777"
     CDS             47909..48166
                     /gene="rpsO"
                     /locus_tag="MJE33_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBY9646745.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            48406..49251
                     /locus_tag="MJE33_000778"
     CDS             48406..49251
                     /locus_tag="MJE33_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBY9646746.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFITEGYHIICMTGAANASGTFQSAMIA
                     KNDVNENEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEEIIDELENTRSSVRLLFA
                     PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDIIL
                     EMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDILA
                     ISCSN"
     gene            49376..51487
                     /gene="pnp"
                     /locus_tag="MJE33_000779"
     CDS             49376..51487
                     /gene="pnp"
                     /locus_tag="MJE33_000779"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBY9646747.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF
                     IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFEETLVDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG
                     MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA
                     RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL
                     SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ
                     MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK
                     IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG
                     KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID
                     EKGRVNLSRKVLLPKPEHKNK"
     gene            51753..52496
                     /locus_tag="MJE33_000780"
     CDS             51753..52496
                     /locus_tag="MJE33_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBY9646748.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDKSGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEDNYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKPGKLSDVFK"
     gene            52654..53901
                     /locus_tag="MJE33_000781"
     CDS             52654..53901
                     /locus_tag="MJE33_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBY9646749.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGT
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQINAFTSKECTCYYVKLIDEHIDTGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAMCLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLIKKEIENIRENYLTEKEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINNVNINSIKKVVDK
                     VFNIENIGTCIVGRDVEKILHLD"
     gene            53936..54181
                     /locus_tag="MJE33_000782"
     CDS             53936..54181
                     /locus_tag="MJE33_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBY9646750.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGGILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            54191..55414
                     /gene="dapG"
                     /locus_tag="MJE33_000783"
     CDS             54191..55414
                     /gene="dapG"
                     /locus_tag="MJE33_000783"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBY9646751.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCSAVNDEPSKRELDLIMSCGEIISGTILANLLNAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKVKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEDNIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKLITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            55527..56261
                     /locus_tag="MJE33_000784"
     CDS             55527..56261
                     /locus_tag="MJE33_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HBY9646752.1"
                     /translation="MKDYREDNERFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGLKEALKKLRELIKESEEEKNND"
     gene            56384..58795
                     /locus_tag="MJE33_000785"
     CDS             56384..58795
                     /locus_tag="MJE33_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBY9646753.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIAGGWLISIF
                     ALILSVMFIFNISIKDLVSNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGLNNDDEEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEGT
                     QSMPELDVLKELQKATNENPVVDTKPEKKVDIAKPNLNIEKTQPMSIVAEPVNEDYSN
                     YKKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYEI
                     QPSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVLE
                     SEEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILYK
                     ANPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFAD
                     AQVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLIV
                     ATQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGAA
                     KPVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKGSDEDEFLSEA
                     IEFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDLQ
                     NLEGE"
     gene            58798..59856
                     /gene="mnmH"
                     /locus_tag="MJE33_000786"
     CDS             58798..59856
                     /gene="mnmH"
                     /locus_tag="MJE33_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HBY9646754.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNDVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESIFFSKENYIFVESESK
                     RVGSVTVPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNILILKECINKF
                     RKRLGNKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYSKIINFNDTQKA
                     LEDVIDLYESILEGESEC"
     gene            59850..61184
                     /gene="rimO"
                     /locus_tag="MJE33_000787"
     CDS             59850..61184
                     /gene="rimO"
                     /locus_tag="MJE33_000787"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HBY9646755.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIV
                     NTCGFIESAKQESIDTIINFAELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILKELSEMHKIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA"
     gene            61171..61713
                     /gene="pgsA"
                     /locus_tag="MJE33_000788"
     CDS             61171..61713
                     /gene="pgsA"
                     /locus_tag="MJE33_000788"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBY9646756.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLIMLLNIPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVFLLLGAQFENVLILNIGEILILIATLL
                     TLYSGWEYLYKNKELFMSSK"
     gene            62001..63047
                     /gene="recA"
                     /locus_tag="MJE33_000789"
     CDS             62001..63047
                     /gene="recA"
                     /locus_tag="MJE33_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBY9646757.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEIDEKVRAFYNLNEEH
                     EESGTSVSKEIVEE"
     gene            63223..64764
                     /gene="rny"
                     /locus_tag="MJE33_000790"
     CDS             63223..64764
                     /gene="rny"
                     /locus_tag="MJE33_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBY9646758.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
     gene            65078..67414
                     /locus_tag="MJE33_000791"
     CDS             65078..67414
                     /locus_tag="MJE33_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBY9646759.1"
                     /translation="MKKNTLLHTNIMVCIIITLGFVATSIIGYQSNTNIFKNDIEHVS
                     TLAAEGIYYQIDKLLSEPINVSLTMANDSLLKNFLDGEKEHLNDEEFIYKLQDYLNVY
                     RNKYSYDSVFLVSTKTNNYYHFNGLDRNLSANNSENQWYYTFLKNDDEYSLNVDNDEA
                     SNNSITVFVNCKIKDDNGATMGIIGVGLKVNSLQMLLKGYNDKFDVVARLIDDKGFVQ
                     LAVDKTGHENINFFENSSSDLSNSKQLILNNKKEQKSFWYSSEKSKSYIVCQYIPSLK
                     WHLVLENDFTLMIKQLHLQFFKGIAVIVLIIIFVLFTITHVLKKYKNQIIKLSVSQKL
                     EYQKLLSKSTEGLYENIFEIDITNNKAGCEDENRYFHEFGLGLNTPYDEALHTIAKNQ
                     IKEEYAQGYLDTFLPENVIKRYNSGINNISYDFLIKKDEESYHWIRINARIFFWTSDE
                     SVRMISYRKNIDEEKNRELLLLERSQRDSLTGLYNKATTEEFIRSFLKNESNQDLEHV
                     FLIFDIDNFKNVNDKYGHVFGDFVISEFALELKSQFREDDIVGRIGGDEFVVLIKDFN
                     NNNYVLIDKLERFCSKINQKKFVEQAEFSIACSIGVAMYPEHGYVYSELYEKADQALY
                     YAKSHGKNSYHIFSEDSIDSISFHIDSRDIKVLLEMATDGVVKFACMENLKLLYFNQK
                     MLEFTGYSAQTVTSIGFDIFSPVLQDDLQMVLDTLREAISKRETFEIVFRFCHTDGHI
                     FPVRMQGKFVSELYQNRYPVFYAIYTDISSINTLLENK"
BASE COUNT        27366 a         7071 c        12429 g        20719 t
ORIGIN      
        1 ataaagaaaa aaatctaact tgattattta ttataattag aaataatatt atcatagtta
       61 tgaccggata caatgtattt tcaacatttt tcaaatagct tacaactaat ataaataaag
      121 ataaatatcg tataaaagca actaattttt ctctgttcat taaaggcact cctccaaaaa
      181 aataaacttt tcctatgaaa actagaataa tatcataaaa gaataaagtc aatagaagta
      241 tttattaata ttgatggaaa taaaatcaat ccaaaaactt gaatagtaaa attgtatact
      301 gaccataatt taaaagtgaa aatttaatgt attggagtga ataatgaata ataaagaaaa
      361 aggtgatttt ggagaaaaag tagcagttaa ttatttgcta tcaaaaggag caaagatact
      421 agaaaaaaat tacagattaa agatagggga aatagatata atagcaaaaa tggaagatga
      481 aattatattt gtggaggtga aatcaaggtc taacataaag tttggctacc cttgtgaatc
      541 tgtaagcttc aaaaaaagaa aaaagataat tggagttgca agttattata ttataaaaaa
      601 taacttaaat aacactccca taagatttga tgttatagag gtatatttat tagaaaaaag
      661 aataaatcac ataatgaatg ctttttaaaa aagataatgt ttttggaaag gattaaatat
      721 gttaagtatt attaattcaa gcaacttagt aggaatcgac agctttttag ttaaggttga
      781 agtagatgta agcaatggaa tacctagttt taatattgtt ggtcttccag gcaaagaaat
      841 taaagaagca agagaaagag taaagtcagc aattcttaat agtggatata aatttccaag
      901 cacgagaata gttgtaaatt tatcaccagc agatataaaa aaagaaggag cttttttaga
      961 tttatctata agtataggcc ttttaagaga gttaataaaa aaagatgaaa attacataag
     1021 agaaagtatg tttataggag aattatcatt agatgggaaa ataagaaaag taagaggtat
     1081 attgcccata attatgggtg caaaaactca gaacataaaa cgtattttta taccaattga
     1141 gaacataaaa gaaagtctat ttgtagatga aattgatata attcctatta aaagtttaaa
     1201 agaatgtgtc gattttttaa acgaagagat aaaagtcgat aaagtaagga taatgtcttt
     1261 tttggatgat aagtcaagaa aagagaacga agaattagaa aaagataata gttatataga
     1321 ttgcaaatat actaagatta ataatgaaga aagtaaatat gatgaagact ttaaagatgt
     1381 aaaaggaaat tattttgtaa agaggagtgc agaaattgct gctgctggaa atcataatat
     1441 gtttatgatt ggacctccag gctcaggaaa aactatgatt gcaaaaagag taaggacaat
     1501 tttaccagat ataagtgtag aagaaatgat agaagtaagt aaggtctata gtatattggg
     1561 tatgataaat gaaactaaag gaataattga taaaagacct tttagagcac ctcatcatac
     1621 aactacaaag caatctttaa ttggaggagg aatggatgca agacctggag aaattgcttt
     1681 agcacataga ggtatcttat ttttagatga gatagccgaa tttgatagaa aaatacttga
     1741 gactttaaga caacctatag aagatggata tgtaaatata tccagagtaa agtacagtgc
     1801 aaaatatcct tgtagagtac tgttagttgc agctatgaat ccatgtccat gtggttatta
     1861 tatgtcagac acagagtgta gatgtagaag taatgaaatt gatagataca taaataaaat
     1921 atcaggtcca ttgttagata ggtttgatat ttttgtagaa gttaattcta taaaatattc
     1981 agattttaat tctttaaaac aagaagaaag ctctcaaaaa ataaagagaa gagtagaaaa
     2041 tgctagaaaa atacagataa atagatttaa gaaggataat ataaaaaata atagtgaaat
     2101 taaagcttac aatttattta aatattgtaa actggaaaaa gaagcttcaa aaacggctga
     2161 gatgatattt aataaataca acctaagcag tagaagttat acaaaacttt taaaaatggc
     2221 aagaacaata gcagatttag aagaaagaga cttgataaat tcacagtgta ttatagaagc
     2281 tttttcattt agaaaggctt attatagtta ttttaaatag ttttttgagg tggattttat
     2341 ggaaaaaaga gatacttatt tatggttaaa atcaattggt ggaataacaa caaaaacaat
     2401 tgaaataata gaaaatgaaa tagtaaatat tgaagatata tttgattttt ctgaaaaaga
     2461 aatatataat ttaaaaaata taagcttgaa tattagaaaa aatatagtaa aatatagagg
     2521 tcacgcttat ttagaaaata taaaagaact actttacaaa aaagcgatta aatatatttg
     2581 taaatatgat aaagaatatc ctgaaaattt aaagaatata tacaacgctc caaagttatt
     2641 attctacaaa ggtgacatag gcttagttaa taataatttt aatatagcta tagtaggttc
     2701 aagaaaacct acagcctatg gaattaattg tgcaaaaaca ataagttgtc aactatccca
     2761 atatggagtt aatatagtaa gtggattagc tataggaata gatgcatact cgcacatagg
     2821 ttgtatgagt ggaaaatcta aaactatagc agtacttggt tcaggagtag acaatcctct
     2881 tccaaaacaa aatttacatt tatcaaataa aatattagaa aatgggggat tattattatc
     2941 agagtataat attaactcga cagtagcccc gtaccatttt tctaatagaa atagaattat
     3001 cagtggttta agtgatggag tagttgttgt agaagcagca ataaaaagtg gtgcactaat
     3061 cacagtagat tttgctcttg aacatggaaa aaatgttttt gcaatacctg gtaatataaa
     3121 ttctcaaatg agtagaggtt gtcataaaat tataaaagaa ggtgcaaaat taattgaaaa
     3181 tatagatgat attttaaatg aatataacat atttaatatt attgataaaa aaataaatca
     3241 aaaatatgat aatataagtc taaatgccaa gtctaagcaa ataattgaag ctataaaaag
     3301 agaaggaaac ttgcatatag atagtatttg tgattatact ggtatagaaa taaagtatgt
     3361 aaattcaata atcaatgaat tagtactaaa tgagttagta gtagaaatga ataataaaac
     3421 gtacagttta aatgtataaa ttaagattag gtagtggggg tgtatggttt tatggccaaa
     3481 acattagtca tcgtagaatc gcctgcaaaa gctaaaacta tagaaaaatt tttagggaaa
     3541 agtcattaca cagttaaagc atcagttggt catgtcagag atttacctaa aagtaaatta
     3601 ggtgtagata tagaaaataa ttttgaacct caatatataa atataagagg aaaaggcgat
     3661 gtaataaaag aattaaaaaa agaagctaaa aaatctaaac aagtatatct agcaactgac
     3721 cctgatagag aaggtgaagc tatatcatgg catttagctc atatactaaa tttagatgaa
     3781 tctgaggatt gtagaataga gtttaatgag ataactaagg atgcaattaa aaaagcaatt
     3841 aaacatccta gaaatattga tataaatttg gttgatgcac aacaggcaag aagagtgcta
     3901 gacagacttt taggttatca aataagtcct atattatggc aaaaggttag aaaaggtctt
     3961 agtgcaggta gagtgcaatc tgttacaaca aagttaattt gtgacagaga aaaagaaata
     4021 aaggcattta ttccaaaaga atactggaca atagatgttg aggctaagac cgtaaaagac
     4081 aaagatataa ctttgaaatt ttatggtaaa aatgatgaaa agattgaatt agaaaatgaa
     4141 gaagctgtaa atcaaatact aaaagaaatg gaaggtaaag atttaaaggt tgagaagata
     4201 gaatctaaat caagacgtaa atcagctcca aaaccattta caacaagtat gcttcaacaa
     4261 gaaggagcaa ataagttatt ttttactact aaaaaaacaa tgatgatagc acaagaatta
     4321 tatgaaggta tcgacataga aggtgaaggt actgttggtc ttatatcata tataagaact
     4381 gactctaaaa gaatatctga tgaagcaaaa gaaaaagcta agggctatat cgtaaacaat
     4441 ttaggagaaa actactataa aaacactggt gataaaaaag aaaaaaagga tactaaaaag
     4501 gttcaagatg cccatgaggc aataagacca acctcagttg atagaactcc tgagagtata
     4561 aaagcctcat tgagcaaaga ccagtacaag ttatacaatt taatttggag aagatttgta
     4621 gctagtcaaa tggaagattc agtgtttgat atactaaatg tagaatgtaa aataggcgat
     4681 attgtattta aggcaactgg ttcaaaaatg aaatttgatg gttataccaa ggtatataat
     4741 ttttcagaaa gagaagacaa aattttacca agtatagaaa aaggagatat attaaatata
     4801 gaaaatatat tgccagacca acattttaca caaccaccag caagatatac agaagctagt
     4861 ttagtaaaaa cactagaaga actaggtata ggtagaccaa gtacgtatgc tccaacaata
     4921 gctactatac tgaatagaga gtatgttgaa aagcaaggaa caagtctatg tccaacagaa
     4981 cttggaataa tagttactga tattttggaa aataatttcc ataaatttat agatgtagat
     5041 tttactgctg atatggaaag taaacttgat gttatagaag aaggcaatac agactggaaa
     5101 aagattgtat cagaatcata tgcaccactt aaagaggcta ttgaagaagc tcttgaaaat
     5161 atagaaaaag taaatatgga tgaagaaact gatgagattt gcgagaactg tgggtctaat
     5221 atggttatca aatatggtag atttggaaag tttatggctt gtaaaaacta tcccgattgt
     5281 aaaacaacta gaccaattat gaataaaatt ggtgttaaat gtccaaaatg tgaagaagga
     5341 gatattatac ttagaaaatc taaaagaggt aaagcttttt atgggtgttc taattatcca
     5401 aactgtgact ttgtagcttg gaataagcca acaggagagt tttgtgagaa gtgtggttca
     5461 tatatggttg aaaaagtgac taaatctgaa acaaaaacag tgtgttctaa taaagaatgt
     5521 aataaagaga caaaagagca taatgacgga aaagagtgaa aattgaaaaa aatcacataa
     5581 ttctcttgaa aaaggttcca atctatgata atctttcaag tgtaaacaac taatagaaaa
     5641 ataaattttc attaaatgtg aaaataatat tagaaaatat aaaaggttat taaaaggcct
     5701 aaatttcatg aggagatgat taaatggcaa gtgaagtgtt acaaaaaaca aggaaaataa
     5761 ataaaacatt acaaacaagt ggtggaagca gtgtctcttt tgatttactg gccggagcat
     5821 tgggcgacgt tttaagttct aatgtttatg tagtaagtgc aaaaggtaaa gtactaggtc
     5881 ttcatttaaa tgatgttcaa gacagttcag ttatagaaga tgagtatact aagcaaaaga
     5941 aattttcaga tgaatatact caaaatgtgt taaaaattga tgaaacatta gaaaatttaa
     6001 atggtgagaa gatattagaa atctttcctg aagaacatgg aagattacaa aaatatacta
     6061 cagtagttcc aatattagga agcggtcaaa gattaggaac attggtactt tcaagatatt
     6121 caaattcatt caatgatgat gatttagtaa tagctgaata cagtgcaact gttgttggtc
     6181 ttgaaatatt aagagcaata ggtgaagaat tagaagaaga aatgagaaag aaagctgtag
     6241 ttcaaatggc aataggcact ctgtcctact ccgagcttga agcagttgaa catatttttg
     6301 ctgaattgga tggaaaagaa ggtctacttg tagcaagtaa gatagctgat agagttggta
     6361 taactaggtc tgtaatagta aatgcactta gaaaatttga gagtgcaggt gtgatagaat
     6421 caagatcatt aggtatgaaa ggtactcata taagaatact taatgacaaa cttacagatg
     6481 aattaaaaaa attaaaaaac aatcaataat ctatatttta taggtttaga ttagaaaagg
     6541 tatctatttc aaatagatac cttttttaga cgaattatta aggtaatgat aagtatatta
     6601 aaaaggagtg agaaaatgcc tttagcagat aaaataagac caaaaacaat gaatgatgta
     6661 ataggtcaaa gtcatataat aggtaatgga aaaatattgt ctaagatatt acaatctaat
     6721 tttttaccaa atatgatatt ttttggtcct cctggtgttg gtaaaactac agtagcggaa
     6781 ataatagcag aaagaagcaa taaaagcttc tataaaataa atgctactaa ttcatcttta
     6841 gaagatataa aaaaggtgat atcagagctt ggaagtataa ataatgtaaa tggagtccta
     6901 ttatatatag atgaaataca aagttttaat aaaaaacagc aacaatcaat attagaattt
     6961 atggaaaatg ggagtataac tttgattgct agcactacag aaaatccata tcattatgta
     7021 tataaagctc ttttaagtag gtctactgta tttgaattta aacctttaga aaaacttgat
     7081 atagaaaaag gactgaaaag agctgttgaa gtattaaatg aagatagcta tatggatatt
     7141 gagtgcaata ataatgctat tgaagatata gcaattcttt ctgatggaga tatgagaaga
     7201 gccttaaata ttttagaagt ggtagtctat tcaactaaac caaataagga taatataact
     7261 tatatagatt cagatgtaat aagggcatct acatttaata aaattataaa ctatgacaaa
     7321 aatggtgata gtcattatga tattttaagt gcatttcaaa aatctataag aggaagtgac
     7381 ccacaggcat ctatacacta tctagcaaga cttataaaag gtggggattt gataagtata
     7441 tgtaggagac ttttagtaat agctagtgaa gatataggtt tagcttatcc aaatgctatt
     7501 gtaattgtaa aggcatgtgt agactcagct atgcaacttg gatttccaga agcgaaaata
     7561 ccattagcag aagctactat tcttttggct acatctccaa agtctaattc tgcatgtata
     7621 gcaataatga aagctatgga tgaactggat aaagagtttg ttaaggatat accaaatagt
     7681 ataaaggatg ctcactattc tggttctaaa gaaataggta ggggttgtgg atacaaatat
     7741 cctcataatt ttaaaaatca ttatgtaaag caacagtatt taccagatag cataaaagat
     7801 agtatttatt acataccaca agaaaataaa actgagaaaa atataaaaaa atatttagaa
     7861 tatctaaata gtgattttta gactgaactt tatgtatcgg gggtagatat tatgattata
     7921 aaacttaact caacttatca cagtagagtt atgaaatact taaaaaaaga acctgagtac
     7981 aatttgttta taatagggga tattgaaaga tatggatatg gaaataattt tttaaatata
     8041 tgggcagatg tgggtgaaca tggtgaaata aaggcaatat tacttaaata ttttgaattt
     8101 atgatgtttt attctgatgg agagtatgat gttgaaggtt tttataatct acttcgtaat
     8161 actaattacg aagaaatatc tgggaaaata tgtgctgtag atgctttagc aaagagatta
     8221 ggacttaata atttaaaggt agttgatttt tgcaaacttc aaactaaaaa gtttttgata
     8281 gataataatt gtaatgcaaa ggttaagaga attaggcttg gaaatctaaa aaagacagta
     8341 aaactttatg acttaataga tgaatttcac agtacaactt tagagaattt aaaaaatgga
     8401 ctaaggacag gtagaggata ttgtatagag attaacaaac aggtggtgtc aatggcaaag
     8461 agtacttcag aaaataggac tcatgcaatg ataattggag taggaacaca tcctaaatat
     8521 agagctaaag gacttgctac aaaatgccta attaaactat gttcagagtt actgagagaa
     8581 aacaaaattc cttgtttgtt ttatgataat gaagaggctg gaaagatata taaaaaacta
     8641 ggatttgaaa acattggaaa atggggaatt tattcaaaat aaaaaattaa aattaatctt
     8701 gacaaaatta gtagggtata ataaaattat cttgaaagta attcctacta aaacagtatg
     8761 aattagattt ctatatagag gtgataatat atgaagttgt ctactaaagg tagatatgga
     8821 ctaaaagcaa tgtttgaatt agcattaaat caagacaatg gaccagtatc attaaaattt
     8881 attgctaaga agcaaaaaat atcagatcag tacttagaac aaatattttc atctttaaaa
     8941 aaatcgggtc tagtaaaaag tgtgagaggt gctcaaggag gttatttgct ttcaaagaat
     9001 gctgaagaca taacagtagg agatatactt gtagtattag aaggaccagt tgcattatct
     9061 gactgtgtac tagatgaaga tgtatgtgaa aactcaaaca tgtgtgtaac aaaaatagtt
     9121 tgggaaaaga tgaaaaaggg tatagaagat gttatagatt caattactct taaagatatg
     9181 ataaatgact ataacaaaaa taaattagaa aatgatataa caaatattaa aaaatagatt
     9241 tggagatgaa tgtaatggaa aaaagaagat tgtatatgga ttattctgca acaacaccta
     9301 ttaaaaaaga agtattggat gcaatgatgc catacctaac agattatttt ggtaatgcat
     9361 ctagttttca cacattcgga agagaagcaa aagatgcttt ggataaagca agagaacaag
     9421 tagcagccct tataaatgct gaacctagtg aaatatattt tacagcaggt ggttcagaaa
     9481 gtgataactg gacactagag ggtgtagctt atgctaataa aaacaaaggt aatcatataa
     9541 taacttcaaa aatagaacac catgcaatac ttcatacatg tgaatactta gcaaaacatc
     9601 atggatttga aataacttat ttagatgttg atagcgaagg aaaagtagac ttaaagcaac
     9661 tagaagattc aataaaagat actactatac ttataagtat aatgtttgca aataatgaaa
     9721 taggaactat acagcctatt aaagaaatat ccgaaatagc taaaaaacat aagatattat
     9781 tccacactga tgcagtacaa gctacaggta atattccagt agatgttaaa gaattaggaa
     9841 tagatttgat gagtatgtct tcacataaaa tatatggacc aaaaggtgta ggagctttat
     9901 acataagaaa aggtgtaaga cttcataact ttgtccatgg tggagcacaa gaaaaaagca
     9961 aaagagctgg tacagaaaat atacctgcaa tagttggata tggaaaagca gcagaattag
    10021 ctaaggaaaa tatgcaaaat catgtagaaa ctttaactcg tcttagaaat aagttaatag
    10081 atggtgtgtt agaaagaata ccttacacaa gagtaaatgg tagcttagaa aatagattgc
    10141 caggaaatgc aaactttgca ttccaattta tagaaggtga aggaatactt ttattattag
    10201 atatgttagg aattgctggt tcaagtggtt ctgcatgtac ttctggttca ttagaccctt
    10261 cacatgtact tcttgcaata ggtttaccac atgaaatagc acatggttca ttaagactta
    10321 cagttggaga ttttacaaca gatgatgata tagattatat actagagaac ttaccaaaag
    10381 taatagaaag acttagaagc atgtcaccac tttatgatga tgcgaagaaa caaggtttag
    10441 taaaatagac ataaagataa agcataaaaa atagacataa attgagtagg aggaaaagat
    10501 aatgcaatat agtgataaag ttatggaaca ttttatgaat ccaagaaata tgggagaaat
    10561 tgataatgca agtggtgtag gtgaagttgg aaaccctaca tgtggagata taatgaagat
    10621 atttttagac atagatggag atgtaataaa ggatgttaag tttaaaacat ttggctgtgg
    10681 ttcagctata gcaagttctt ctatggcaac tgaaatgata aaaggaaaaa ctataaaaga
    10741 tgcattagaa cttacaaata aagctgttgc agaagctttg gatggtttac caccagtaaa
    10801 gatgcattgt tcagttttag ctgaacaagc tgtaaaagca gctcttatag attatgctca
    10861 aaagaacaat atccatatac cagaattgga tggatatgtt atagatgatg ctcatgacca
    10921 tgatgttgaa gaagaagaat aaaaataagt gattgtgtta tgagaagttt acaatgatta
    10981 aaaagaagta ctaatgatat aattaggaca taaaaaatag ctgtttttaa aatggctatt
    11041 ttttattgcc taaattaagt attttaactt gatttagagg ttaattagtt aacaaaataa
    11101 tccataagat tcaaataata aagatgatta tttttttaga aaatagaaca agctatattt
    11161 taaataagta caatacttgc tctatatggt ataataataa gtaatgcaaa agatgaggtg
    11221 aaatttgttt atgaataaaa aagtaatgat aggtatgagt ggaggagttg atagctctgt
    11281 agctgcatat cttttaaaac aacaaggata tgatgtcatt ggtgttacta tgaagttgtg
    11341 gcaagatgat gatgttgttg aaattgaagg tggttgttgt tctttatctg ctgttgaaga
    11401 tgctagaagg gtagcaaata aaataggtat accattttat gttttaaatt ttagagaagt
    11461 gtttaaagaa aaagtaatag attattttat agatgaatat ttggaaggaa aaactcctaa
    11521 tccctgtata gcttgtaata aacatataaa atttgatgat ttttataaga aggctagaca
    11581 aataggttgt gattatgtag ctacaggtca ctatgcaaag atagaaaagg atgaaagtac
    11641 aggaagatat ttgctaaaaa aatctgttac agataaaaaa gaccaaactt atgcacttta
    11701 taatcttaca caagagcaac tagagcatac tttactacca ataggtgact atgaaaaaga
    11761 tagagtaaga gaaattgcta aggaaatggg tatggcagta cacaataagc ctgatagtca
    11821 agaaatttgt tttgttaaag ataatgacta tgcaaattat gtaaaaaaac attctaaaaa
    11881 gcgtattgaa gaaggttttt ttgtagatac taaaggcaat atacttggaa aacataaggg
    11941 tatattatat tatactatag gccaaagaaa agggcttgga attacttttg gaaaaccaat
    12001 gtttgtaata gacataaatc caataaataa taccatagtt cttggagaca atgaagattt
    12061 atttaaaaag gaacttattg ctaaagatgt taactttata tcaatagata cgttagaaga
    12121 acccttaaga gttcaagcca aaataagata ttcagcaaaa ccatcaccag ctactattca
    12181 tagagtagga gaagatacta ttaaaatagt ttttgatgaa gctcaaagag ctattactaa
    12241 aggtcaatct gtagtaatgt atgatggaga tattgtagta ggtggaggta taatcgagaa
    12301 aagtttataa aaacatctaa aaaattaaaa aaataagcaa taaagtatat taagagtaaa
    12361 taaagtttat aaaataaatt tattgtatat gaaaatatag tattgattaa ttttgtaaaa
    12421 tttagtaata taattagtat attaattaaa aaacaaatac agtgataagg aatagtaaaa
    12481 tatttgatgt cttacagaga gagggaaatg ctggaagccc ttagaatatg atatttgaag
    12541 cagcctttta gtagcaatct tgaacaattt atattttata aagaagattt aagtagagat
    12601 tgacggtagt tacgttatag ctaaggtttt ccgaagatat catactttgt atgataatta
    12661 gggtggtacc gcgattgtcc tcgcccctat aaatttattt atagggacga ggttttttat
    12721 ttaaacgaaa aaataaaatt tattaggagg aacatgatat ggaaaagatg ggattaaatg
    12781 aaataagaag taagttttta gaattttttg agtcaaaagg tcattatgta gcaaatagtt
    12841 attctttagt accaaacaat gataagagtt tgttacttat aaactcaggt atggcacctt
    12901 tgaaaaacta cttttcaggt gtagaagtac ctccaagtgt gagaatgtgt acatctcaaa
    12961 aatgtataag aacaggagat atagaaaatg taggtataac tgcaagacat gctacattct
    13021 ttgaaatgat gggtaacttc tcatttggag attactttaa aagagagtca atcaaatggg
    13081 gatgggaatt tgttacagaa tggctaaata taccagaaga taagatatgg gtgactgtat
    13141 atgaagaaga tgatgattct tatgatatat gggcaaaaga aatgaatttc ccagaagaaa
    13201 gaatggttag acttggaaaa gatgataatt tctgggaaat aggtacaggt ccttgtggtc
    13261 cttgttcaga aatatatttt gatagaggag aagaatatgg atgtgacaat ccagactgta
    13321 aaccaggctg tgattgtgat aggtatttag aattttggaa ccatgtattt actcaatttg
    13381 atagagatga agaaggaaat tatagtctat tagaaaacaa gaatatagat acaggtatgg
    13441 gtcttgaaag aatgggatgt attatgcaag gcgtagacac tatctttgaa gttgatacaa
    13501 ttaaatctat actagaagca gttgagaagt taacaggagt aaaatatgga gagaatccaa
    13561 aaaatgatat atcaataaga ataataactg accacataag agctgtaaca ttcttagtta
    13621 gtgatggagt gcttccatca aatgaaggaa gaggatatgt tcttagaaga ctccttagac
    13681 gtgctgctcg tcatggtaag ttattaggag taaaagaatt atttttacag aaactaatag
    13741 atgaagttat aaaagtaaat gataaagcat atccagtttt agttgaaaaa gaaagttata
    13801 ttaaaaaagt agttggaata gaagaagaaa aattcaatga aactatagac caaggaacag
    13861 aaatattgaa ctcttatata gaagtattaa aaaatgaagg aaagactgtc ctaagtggtc
    13921 aagaagcttt taaactatat gatacttatg gcttccctat agacttaact aaagaaatat
    13981 tagaagaaga acatctatct gtagatgaag aagcttttaa tgaagaaatg gaaaagcaaa
    14041 aagaaagagc tagaaatgct agaggaaata tggatagaga aagttggaag gaagaccctc
    14101 tatcaaaatt agaatctaca gttgacagta catttaatgg gtatagtgaa atttatggtg
    14161 aaggaactat tgaagctata gttaaagatg atgagctagt tcaaagtgca gaagaaggcg
    14221 ataaggtatc tatagtacta gataacacta ctttctatcc tgaaggtgga ggacaagttg
    14281 gagattgtgg attgataact aatgaaaact tagttttaga agtattaaat actaaaaaag
    14341 gtgctaataa tagtataaaa catataggta taataaaatc aggaagaata agtaatgggg
    14401 acaaagtaaa gacactcgta gatagagaaa ctagaatgtc agctgcaaga aatcacagtg
    14461 ctacacattt acttcataag gcattaagag aagtattagg agaacatgtt aatcaagctg
    14521 gttctttagt aactccagaa agacttagat ttgatattac tcactttgaa gcgatatcaa
    14581 atgaagaatt aaaagtaata gaagaaaaag taaataatgt tattttatca tctttagaca
    14641 taaaatgtga tattatgaat ataaaggaag ctaaagaaaa aggagctaca gcactatttg
    14701 gtgaaaaata tggtgatgag gtaagagtag tttctatggg agattattct acagaacttt
    14761 gtggaggaac tcacttgaca aatacttctc aagtaggaat gttcaaaata ttatctgaag
    14821 gtggagtagc agcaggtgta agaagaatag aagcaataac aggaaaagct gtttatgaat
    14881 acctaaaaga aagagatgga attatctctg aagtttgtgt aaacttaaaa tctaaagaag
    14941 ataatttaat tcaaagaata agttctttat tagaagaaaa taaaaactta tctaaagaat
    15001 tgcatgatat gaaagctaag atgagtttac aatcagttga ttctattttt gattctaaag
    15061 ttgaagtaaa tggagttaat ttgataacta ataaatttga aggtatggat atggatacac
    15121 taagagaaac agcagataat ctaagagata agcttggttc tggtgtagta gtacttgcaa
    15181 atgtagtaga tgataaagtt aactttgtag ttactgccac taaagatgta ttagataagg
    15241 gaatacattc aggtaatatt gttagagaag ttgcaaaaat agctggagga aaaggtggag
    15301 gaagacctaa tatggcacaa gcaggagcta gtgatgtatc taaggttgac caagctttaa
    15361 gctatgcaag tgaggttatt aagacacaag ttaaataaca ttcactcaag gaggttttta
    15421 gtttggaaaa agatttagac tacactatga aatttgaagg aataccagaa gataggatga
    15481 gtgtaggaga tactattgat tttgtatata aagcattagt tgaaaaagga tataacccta
    15541 taaatcaaat tatagggtat ctcctttctg gtgattctag ctatataact agtcataaaa
    15601 atgcaagagc tataattaaa aaatttgaaa gagatgagat acttgaagaa gtaatcacgc
    15661 actatctgaa tagaaagtag gtcaaataaa tgagaaaaaa gattatgtcg ttgtttgcaa
    15721 tagtcattat gatttttgta tcaactgcta gctatggttt tgctgaagaa aaaggcaagg
    15781 tcatatttat tgatatgaat aggaccaatc ttggaagtat gatgaatatt ccaatcttga
    15841 aaaatgagat agaaaagaga ggctatgtag ctctcatgaa tataagaggg gatcaaggta
    15901 ctgatgatag aaggtcttat gcaagtatgg gtgcaggaac aagagctagt gtaactactg
    15961 aagactatat caattttcaa aaagtagacc aagatactgc taaagctttt gaagtcgcaa
    16021 caggtcaaaa agctaaagaa ataaatgatt taggcattaa tagatctata aatgaaaatc
    16081 ttgagtatgg agaatatgga gcaaatttag ggtcattagg aaaaacttta tcagaaaatg
    16141 gtttaaagac atctgtaatt ggaaatgctg atataattga aaataatcaa ttagttaaaa
    16201 acagaaactt atgtttagta gctatggacc aatatggaag gattgattca ggtaatattg
    16261 ataatattaa taagaaagat tctaaaatgc catttggaat atctacagat tatgataaat
    16321 tactagatga aactaaaaaa tcctataaaa atagtgatgc tatatttgta gaacttggag
    16381 atacatatag attagatatg tatagattaa atctaaatga taatacttat gatacaatga
    16441 agaaaaatat tgtaaaaaat ataaataaat atcttgaaca agtattttct atggttggag
    16501 aaaatgacac tgtttatata gcaagtgcat ttccaagtga cctagattat aagaataaga
    16561 gaagactttc tccaataata aaatttagtg gagaagggaa aggtctactg gaatctgcca
    16621 caacaagaaa ggctggaatt gttgccaatt tagatgttgg agtagatata ctaggtacat
    16681 ttggtcttga aaataaagag atggtaggta gaagttatac tttagtaaat aaagaggaca
    16741 atattagcta tctaaaatca ctttatgaaa ggatagtgtc tatatcttca ataaggtcaa
    16801 ctattataaa tggctttgta ggcgtaatat ctgcttcttg ggtaattgcc atgtttgcga
    16861 ttttctttag aaattatatg ccacataaag agcgtgtatt caaagtactt aaggagttta
    16921 taaaattagg cataataatg ccactatcat ttatgttagc tcctttgatg aattttaaaa
    16981 ctccaatttc aatatctttg ggaatatttg cgactacgct aatattatac ttgttaggta
    17041 gaaaattatt taaggacaat gatataaagc acatgggatt ttttgcaaca cttactattt
    17101 tagtcatagt tatagattct gtatttggaa catacttaat gcaaaataac ataatgagtt
    17161 atgatgctgt cataggagct aggtactatg gtgtaggaaa tgaatatgaa ggtgtaacta
    17221 tagcaagtgc agtatttggc ttagcagtat tattacatta taagaaggta tctaaattac
    17281 ttgttgtaat tttttcatta ataatactta taactagtgc atatccttca atgggtgcaa
    17341 atgttggtgg agctatatca gaatgtgtag catatttgtt gtttataatg cttatatatg
    17401 acattaaaat agattttaag aaagctgtat tattagctgt ttcagctgta gttgtcgttt
    17461 ttgcatttgc agctttagat atactttcag gtagtgaatc tcatttaggt atgtttgtac
    17521 agagaatttt cctaaatgga ccaggagaga tattacaaac atttggaaga aaaattcaaa
    17581 tgaatatgca actagcacag acaagtgtat gggtaaatat tttacttgta ggaattggag
    17641 taattgcagt gcttatattt aaaccatcta gacactttag aaagataatg aataattttc
    17701 caatcttgtt taaagggttt attgcatcta tggttggttg tataattact cttttagtta
    17761 atgattctgg aatagttgct gcatctacag catctatata tatacttata ccactaataa
    17821 taataagtat aaatatgata atatttgaaa aaaataacta aaaaaatttt tactgttcat
    17881 ataggattta aataaaaaat gcaatatgat tattaatgta aactggatgc ttacataaaa
    17941 aatcatattg caatttttat gattgattat gtaaaattat atgaagcagt aagcctgtga
    18001 ccttagttat gagaggttca gaatatgtat ttaatctata gaatataaat agataaagga
    18061 ggaactatgt tagatggaag aataatgggt cttgatgttg gtgataaaac tattggagtt
    18121 gctgtgagtg atttgatggg attaactgca caaggtgtaa aaacaataaa aagagttggc
    18181 aagaaaaaag atattgaaga gctaaaagca ataataaaag aaaaacaagt aaataaaatt
    18241 gtatcaggat taccaaaaaa tatgaatgga actttaggtc cacaaggaga aaaagtaatc
    18301 aagttttgtg aattggtaaa agcagaaaca ggtattgatg tagagttttg ggatgagagg
    18361 ctttctacag tagcggcaga gaggtcttta ttagaagcag atgttagtag acaaaaaaga
    18421 aaaaaggtta tagatatgtt ggcagcagta ataattttgc aaggatattt agattttaaa
    18481 ataaattcat aaaagaacta taaaattgaa tacttatgat atataatata aataataaaa
    18541 ataaaaataa atttgaggtg aatgacatta tggaagaaaa tataataaat ttaatagacg
    18601 aaaatggcgt tgaaagccaa tttgaaataa tattaacatt agaagcagaa ggaaaagagt
    18661 atgcaatatt aatgccttta gatgatgaag aagctgaaga agctttaatc tttagaattg
    18721 atgaggatga agaaggtgaa atcttaatac ctttggaaag tgatgaagag tatgaaactg
    18781 ttgtagctgt gtatactgct ataatggaag aagaaggttt aaattacgac gaagatgaat
    18841 caaatggact taactagata agtaattaag tttgtatata aaaatcagat atttattata
    18901 tttgctttaa ttaaacctta ttaattgtaa aagcatttat agtatatatc tgtttttttg
    18961 ttttttatta aaaataccaa aaaatttatg aattttggtg aataatatat cttttatggg
    19021 tatatattta tattatgaga ataaattaaa tttatttaaa tgtcttgaca aaaaatgaat
    19081 tatcaattac aatattattt aataaagatt atcaattgaa aatagtaagg gtgatgttat
    19141 ggcaaataca atggatttat taaaagacaa gttaaaagaa actggtttta agattacgcc
    19201 acaaagaaga gcaatagttg aaatattgct taaacatgac cattcacatc taagtagtga
    19261 ggaaatctat gatttagtta gggttgactg tcctgaaata ggcttagcta ctgtatatag
    19321 aacaatgcag ctattggatg aaataggatt aatatcaaaa ctaaacttag acgatggatg
    19381 tataagatat gagattagtc tacataaaga agactgtcat aatcatcatc atcttatttg
    19441 taaaaattgt ggaaagataa tggaagctaa agaagacctt ttagacaata tagaaaaaga
    19501 gatacaatca ttgtacaaat ttaaaatact tgatcatgat gttaaatttt atggactatg
    19561 tgacgagtgt aatggagtaa gcgattcaga agaataaact tctattgctt aatgtcgatg
    19621 gaagaatagt atagaacttt tatattctat gctattcttc cattttttat tataaaccat
    19681 ctacaaaatg caaatataca gttttcaaga tgcttatagt tttaatattg aaaatataat
    19741 taatcctata agtacaaaaa tgaatgattt ttctatgaaa ttactaaatg gtcctccaac
    19801 agttaaaaaa cctaaagaaa ttttcttttc gcttagcgga taaaaaattg gtattccttg
    19861 ctttgtaaat aaatctccta aaaacatatg acttgcataa ccaatcgtac cataaaaaga
    19921 taaattatta atattagtga ttaattctat ctgttttagt aaaaaatata ctattatagt
    19981 tgcaaatatg ctatgagaaa aacttctgtg ctttaaccag ggaaaaattg acaacattag
    20041 acaaaaaatt gtaaaatata tttcaacttc tatgatgtat aagcatatag ctaatagtat
    20101 aaatattaat gatagaaaaa cttttcttag aaatgtgtgg gttagttttg cttcaataat
    20161 tataattgca acaaatgtaa ttattgcgct taaataaaaa ttatcattta tatttattga
    20221 aataaagaaa ataataatat taacagcata tataaaaatt ctatatatgt atttggatac
    20281 attgtttttt agaatgaaat ttgagaccat agaattagat ttatccaaat cgggtagtac
    20341 agcaaaagta gctgaaacta aaatatcaac taaagatata ggaattttaa atattaaaga
    20401 tgtttggata gctgttaaga ttcctattgc acaatgagtt ttaccacgca taaatcctcc
    20461 taaattatcc gatattaaat catataatat ttagaggata tcaaaataaa ataggtttgt
    20521 caaaataata cgttaaatgt attttaaata tagcttaaaa tatgatacga attatggtac
    20581 aataagaata actggtagcg agtgcgtgat ttgatattat ttatgattgt agaacgtgtt
    20641 aaaaaattaa atagacagaa ggagatgatg caattgttca aaaagaacac caacaagata
    20701 aaggtaatgg ccttgggtgg acttaacgaa gttggaaaaa atatgactgt tgttgagtat
    20761 aaagatgaaa taattgttat agatgctgga cttagttttc cagaggatga aatgttagga
    20821 gtagatatag ttataccaga tataacttat ttagtaaaaa atagggataa aataaagggt
    20881 atatttatta cacatggaca tgaagaccat ataggagctc ttccatatat attaaagaaa
    20941 ataaatgtac ctgtatatgg ggcaagactt agtataggtc ttatacaagt aaaacttaaa
    21001 gaacataaaa tgaataatgt taagttaaat gttataggtc ctagacaagt tataaagctt
    21061 gataatatgg aagtagagtt tttgaaaaat aaccatagta taccagatgc ttactctatt
    21121 gcaatacaca cagaccaagg tataatatac cacacaggag attttaagat tgatttgact
    21181 cctattgatg gagatgttat ggatatgcat agaatctgtg agttgagcaa aaaaggagtt
    21241 cttttaatgc tggcagatag tactaatgca gaaaaaccag gatttactat gtctgaaaag
    21301 actgttgggg taggtcttga cgaattattt gcaaaaggaa atggcagaag aattatagtt
    21361 gctacctttg catccaacat acacagatta caacaaataa taaatactgc tgaaaagttt
    21421 aatagaaaag ttgctatatc aggtcgctca atggtcaatg tagttggagt tgcaaaagaa
    21481 ttaggatacc tagatatttc tgatgacatg cttatagatt taaatgatat atgtaagtat
    21541 gaagatagtg aattagtgat aataacaact ggttcacaag gtgaacctat gtcagctctt
    21601 gctagaatgg ctttttcaga gcacaaaaaa gtagaaataa agagtggcga tttagttata
    21661 atatctgctc atccaatacc aggaaatgaa aaattaatat caagagtaat caatttctta
    21721 tttgaaaaag gagcagaagt agtatacagt gatatagctg atatacacgt ttctggacat
    21781 gcctgtcaag aagaattaaa acttatacat gcattagtta gaccaaagtt ttttatgcca
    21841 gctcatggtg aatatagaat gttaaaaaga catgctgaaa tagctgaaca gcttggtatg
    21901 gataaagaaa atatttttgt catgcaaaca ggagatgttt tagaattaga taaaaattct
    21961 gctaaggtag caaaccgtat acaaacagga aacatattag ttgatggtct aggagtaggc
    22021 gatgtaggta atatagtttt aagggataga aagcatttat cagaagatgg tcttatgata
    22081 gttgtagtta ctatttccaa ggatgagggt aaggtcttag caggtccaga tataatttcc
    22141 agaggattcg tatatgtaag agagtcagaa gatttaatgg atggagcaaa ggatataatt
    22201 aagaatgttc taaatgaatg tgaagagaaa aatattaaag aatgggctta tttaaagaat
    22261 aatattaaag aaaacctaaa agaatattta tatcaaaaaa ctaaacgaaa tccaatgata
    22321 cttcctatca taatggaagt ataatataaa aaaactataa atttgaaaat tagacctctt
    22381 gtaattttta aagtaatatc aaaaatccaa ttgatattat cttaagaatt acaagaggtc
    22441 tttttaatac tcattttaaa aaaatatata ttacataaaa taccaattat aacaatcttt
    22501 tattagtaaa aactacttat atataaattt tactgatagg aggattctta tggatgatgt
    22561 ttcaagacaa aatgctataa aagctttaaa acaaacaaaa atggaaatag caggagagta
    22621 tggaatgaat tatgaagatg cttttgaaat aatagaaaat gcgtctaata aaggtgtttt
    22681 agaaggttac tttaaaaagc ttgaaaagaa aaaaaattta ggtcaaggta tatctaggca
    22741 tttagaataa aaaaagttaa atgtgagaaa ataagtttaa ataaacttta agaggtgaca
    22801 tcgatgaaaa aattagctag tactgctttg gctatattaa ttgcgcttac tcctttaagc
    22861 ttttcttttg caaataataa ggaaaatgct gatgccaacc aactaaatat atcttcaaaa
    22921 tcagcaattt tgatggatgt tggtagtgga caaattttat atgaaaagga tgctcataaa
    22981 aagcttcctc ctgctagtgt aactaaggta atgactatgc tacttatagt agaagctttg
    23041 gattcaggca agattaagct tgatgatgaa gttcaagtaa gtgaaactgc atctagtatg
    23101 ggtggtagtc aaatattcct agagccagga gaaactcaaa aggttgatac attattaaaa
    23161 ggtatagctg ttgcttctgc aaatgatgct tgtgttgcta tggctgaaca cttagctgga
    23221 agtgttgaag gatttgttga tagaatgaat gctaaagcaa aagagttaaa tatgaatgat
    23281 actcattttg caaatacaaa tggacttcca gttgcaaacc actatacatc tgctcatgat
    23341 atagctttaa tgtctagaga actattaaaa cacgaaatga taagtaaata cttaactaca
    23401 tggatggata aggttgtagt agggaagaaa caagttacag taggtcttgc aaatactaat
    23461 aaacttataa agcattatca aggagcaact ggtgttaaaa ctggttttac tcaagaagca
    23521 aaatactgtt tatcagcatc tgcaaagaga ggtaatactc atctagttgc tgcaacttta
    23581 ggagcagaga cttctccaga aagatttaat gatgcctcta gccttttaaa ctatggattt
    23641 gctaattatg aaagtgttaa gttatgtagt aaaggtgata atatagcaac acttacattg
    23701 gataaggctg atgaaaacaa agttaaattg gttgctaaag aagatttaaa tgctcttatt
    23761 aaaaaaggaa gttctaaaga atttgagaag aaaattgaaa tagtaaaaaa tcctaagatg
    23821 ccaattaaaa agggaactgt tttaggaaaa ataaagatat gtaaagataa aaaagtaata
    23881 ggcgaagtgg aattgataaa tactaaggat ataaataagg caagctatct acaaatgctt
    23941 caaagaataa ttgataatat gatataaata attatcagat gaaatataat tataatacac
    24001 aaagatatga ttataataca cataaaaaat acccttatgt aattatttgc cataggggta
    24061 tttttattta ccatatagaa gtattttata atcttatgat ttttatagaa atatagaaaa
    24121 gtttatgtat cttaaaatat catttgattt aatatcatat atagggttgt ttaacaatta
    24181 tctaagtaaa ggcatactac cagtaagttt attcactttc tttttattcc cataaattgc
    24241 tatcccaaaa tactgtaact catcttctgg aacactagca atcttttgtg taaattcttc
    24301 ataaagattg caactttgtg ctacatctga aaaatctaca acagttaaat cgttaaattc
    24361 caaagtatat aattttttac gtaaatcttt tattatttct ttgtttcctt ttaaaatagg
    24421 tacaggaatg tcaataattc ctaagtggct attacctgtt ttgtcaatta catctgggcc
    24481 aacagtttct ggggcatgtt ttcctagtgt aattcccatt atagctgctg tattggatat
    24541 aattcccatt ggtaaatttt cgtctattac catgacacat tttgaatttt tagtttccat
    24601 ttttatttct cctttttacc ttatttaaag ttgtttgaat catcatttat aaatatattt
    24661 tgatactgct ttggtgtaag accaataaaa ttcttaaaaa agtttgtaaa gtgactttgg
    24721 tctacaaatc ctgtttgtat cgcagcatct ataggttcta cgcctttttc taaaagcttt
    24781 ttagctttgt ttacccgtat attttctagg tatctatatg gtgtaatacc ttttaatttc
    24841 gtaaaattac gaagtagaga gtacttattc atacctgcta tagtgcttaa ttcgtcaagt
    24901 actatatgtt cagcataatt attttctaga taatcacata ctgcttgaat ttctatattt
    24961 gtattttcca agttagattg caaatttggc tctgtatgtt cttcaattaa ttgttcaatt
    25021 aggaagaaaa aaagctcttc ctttttaaaa tccaattctt cttccatgat tatatagtgt
    25081 aattcttgta gtagaggtac tagctcactg cgaaatataa caggttgatt aaattttggt
    25141 aaataatttt tacctgtaat ttcaaagacg gtttttttca ttatttctgg cttaatatta
    25201 atacatctgt aatcaagtac tttatcatct atttgttcac aagtatgact atcaaaaggg
    25261 ttaaaaagta gcaaatctcc agtacttgtg gtatactctt tatttttaca agataaatat
    25321 ctttgacctt tttctataaa accaattaca taatgttcat gaaaatggtt tgggaatttt
    25381 tgcattatgc ctttaaatct atatgcttca atttttaatt ccctatcaaa gcatactgtg
    25441 cgttcttctt gtctcactta ggtaacctcc tttcatgtta tacaaagtgt aacacatatt
    25501 tttttatact tcttgtataa tattgactaa gagacaataa tgtcaattca tgtcaatata
    25561 atattatact tacagtaggt gtaattatta tattttcaca atgaatatga tataataata
    25621 aaagttataa aataaaaagc aaattttaat tttatgccta gaagttaaaa ttattataat
    25681 gtaaaataat tttgattata agctatttaa ttgttataat aatattaaaa atttaattta
    25741 ggagatgtac attaatgtta agtatgaata taggagaaat agtagcaagt ttagttggta
    25801 tagctatggc aatatcaata catgagtttg gccatgctta ttcagctcac ttattggggg
    25861 atgatacagc taaagcttat ggtagaatga cattaaatcc tgtaagacat gttgacccaa
    25921 taggtctttt ggctatgttt atccttaaga tagggtgggc aaagccagta ccagtaaatc
    25981 caaataactt taaaaactat aagataggaa acctgatagt ttcacttgca ggcgtttttt
    26041 gtaatgtttt gactgctata ctgtgtgtaa ttatcaataa gtatgtgcat atgtatgcta
    26101 taaatacaat agctggatat gtgcttttat atagtatggg atttgcagct tttaacttac
    26161 ttccgatacc tccacttgat ggatggggag ttatatctac atttgtacca tataaatgga
    26221 atgaatattt gtataaattt gaaagtatga gttatataat tttattaatt gctttattta
    26281 ctgggatgta tagaataata ctatcaccaa tatatacagt gttgctaaaa atagtatatt
    26341 tatttgttta atataataaa cagattaaag agggattgat atgaagtata atatacaatt
    26401 acaagtctat gagggaccat tagatttact ttatgattta ataacaaagc ataaaataga
    26461 tataaaggat atatccataa tagatataac taagcagtat ctaaattatt tgaaaatgct
    26521 agataaaatg gatttagaga taactagtga atttataact atggcatcaa aacttttaga
    26581 gataaagtct aaatatctgc tttataagca aaaagatgaa gaagaagacc caagaataga
    26641 acttatggaa aagttagaag aatatagaaa gtttaaggta gcatcacaag atataaaaga
    26701 aaatataact tatgttaatg agagatttta tagaaataaa gaagagataa ttattgatga
    26761 caatgtggat ttagaagata tatcaattga agcaataaaa aatatacttc catacatatt
    26821 taaagttaaa acatcacaga tagaaaatgc taatgatgag aaactagata aaatagtaag
    26881 gaagaaaata atatctgtag aagaaaaaat attatatata agagatatta taaaagataa
    26941 gattgaagtt acttttacaa atattataaa atcatatgaa aatgatgaaa taatagctac
    27001 ttttttatct atattagagc ttataaaaga aaaagaaata gtcgtagtac aagatatatt
    27061 ttttgatgat attctaataa gaaaaagttc ggagtgttaa aatgaaaaga gaagatataa
    27121 agtatattat agaatctgtt atgtttgcat atggggaacc aataagtata aaagaattaa
    27181 actatataat aaataaagaa ctttcatcaa aagaaataga gattatgtta aatttactta
    27241 ttgaggagta cagagaacaa aatagaggta ttcaaataat caagttagag aataagtacc
    27301 aaatgtgtac aaataaagat tatgcagaat atataaagaa gattattgaa ccaaagaaaa
    27361 agaaaagttt gagccaagct acattagaaa ctcttactat aattgcctac aaacaaccaa
    27421 taacaaaggt agaaatagag gatataagag gagtaaaatg tgataaggta ttacaaactt
    27481 tgtttgaaaa tgaacttata agagaagctg gtagattgaa taagataggg aaaccaataa
    27541 tttataaaac aacagatgaa tttttaaagt tgttgaatat agaaagtctt gaagaattgc
    27601 cacctataga aaattatcaa gaagtggcaa caaatgaata aaaatataaa atcacaataa
    27661 tttagtatgt aattttaaat tttaaaagat ttaaaatgct ctaagttgtt gtgttttttt
    27721 gtataaatat aaaattataa ttctcttaaa ataaatatgg ataattaagt ataaatagta
    27781 attttttgat gataatataa acatggagct taatgaatta tttaatttta agtatttaat
    27841 aatagctgta atttttatta taagtgtaac tatgttgatt ttaatatctc atataaacat
    27901 cttaataata gctgatataa aaaacaaaga tatttgttta aagcttaata taaaatacat
    27961 gtttaattta ataaatataa atagacaact ttatccagct gaaaatagta aaaataatga
    28021 taaaaaagag gggatgaaga ataatataga ttcaagtata ttattagctg atgacctttt
    28081 gagcatttat agattattaa aaaagataaa aatacatgaa ctttattcaa atattaattt
    28141 tggaactgga aatattggct taacatcttc tgtatatgta ttgataaata ctttgtatgg
    28201 aaatttattt aatatgattg atgcagaaaa aatgtattta aatgtaaatc cagattttac
    28261 aaaagattat gttttaggaa atataaggat acatataagg ccaagaataa aagctttatt
    28321 taatattata ataatgatta ataaaattat gaataaaaat aaaggaaata aggaaggtga
    28381 tagtaatgag agcaacaggt tcgatacaga gtcttatgga aacaactctt gaaacaatta
    28441 aaggttctat agatgcaaat actattatag gagaccctat aaaaacagat actacagtgg
    28501 ttgtacctat atcaaaggtt acaattggct ttggtatagg aggaggagaa tactcaaaag
    28561 gatatgatga taaagatagg gaaattgaat taaaaaatga aaaatcagat acgaattttg
    28621 ctggaggtag cgcaggggct atatctgtac aaccagtagc atttgtagta gttgaaagtg
    28681 gagagactag aataatgagt ttagatagta atatcaatct agtggataat atattatcta
    28741 taactcctag agtattggag aaaattcaaa atatatcaca aagtaataag aatcaagata
    28801 aaaacaatat gtaagaataa aatattttta aatatgtata tagttttaag taaagtagtg
    28861 gggttaatat aaatcccact attttgttgt gtactttaaa catttatatc taataagatt
    28921 ttagtttaaa acaatttaaa tagataataa agttgatata tagtataatt ataaaaagaa
    28981 aagaagtatt atattataga tatataaacg catttgagat ataagaggga gttataaatc
    29041 attagttttg aggtgaatgt tatgagcgat aaagaattat ctattttgtt gttagaaagt
    29101 atgaataatt ttcataattt aataaagatt attaataacg aaagatataa aaaagataag
    29161 gttcttacag aaagacagtt ctttgcctta gtaaaaataa gaaaacatga taaaatagag
    29221 cttaaaaatc taagtagaga tttgcatgta tctacttcta gtctgtgtat actacttaat
    29281 aaattggttg agcaagagta tgtatatagg gaagaagata gtagagatag acgaaatact
    29341 ttttatggaa taactaaaaa tggagaaaag atattggata atgaaatact aaagtttgta
    29401 tctataataa gtgataaaat ggattgccta gatatagata ataaagataa attatttact
    29461 tctttggaag agtctaaaaa tataatagaa caattatttt aaaaaggaga atttattatg
    29521 aatataaagt attctaaaga gatattaaaa agtttagaaa atgctataac agtagtagct
    29581 ctatttgagg gagaagatgt ttttacgaca tttgataaaa taaatatgat aagcagaaat
    29641 tttaattcta aagaaaatag tatagatact gtaacattaa ttgaaaataa caatttggta
    29701 aatgtttgtt ttgttggttt tggtaagaga gagttattga atagagaaaa aattagaatc
    29761 ataggtggga atttgtgtaa aaaattaaaa aatatattga aaaatgaagc atataagagt
    29821 agtgatgtta atgtactgaa tcttaattta aatagtgatg aaataggttg ttttgtagaa
    29881 gggatattac ttggaaatta taagtttgat aagtataaga caaaaagtaa ggattttgaa
    29941 agaaaagaag ttgatactat aactatattt actgaaaaag aagttgagtg tcaaatagaa
    30001 aaggctaaaa tattagctaa ttcaactata atagctagaa atctagttaa tgaaccatca
    30061 aacgtaatat atccaaaaac attagcagta gaaacagtta aacttggtgc agagtttggg
    30121 tttaatgttg atgtttatga agaagaaaaa ataaaggcat tgggaatgga tgcttttttc
    30181 gctgtttcaa gaggttcaat taataaacca agattcatag taatgagata ctttggagat
    30241 aaagaaagta cagatatatt aggtctagta ggtaaaggat tgacttatga tactggtgga
    30301 tattctttaa aatcaaatgc ttctatgcta gatatgaaga cagatatggc tggtgctgcg
    30361 agtgttatag gtgctatgtg tgctatatct caaagtaaat taaagaaaaa tgttatagca
    30421 gtagtggctg catgtgagaa tgctttatca ggaggttcat acaaacctgg agatataatt
    30481 agttctatgg caaagaaaac tattgaagtt ttaaatacgg atgcagaagg tagacttaca
    30541 ttagctgatg caatatacta cattataaac aatgaaaaag taacaaaggt ggttgatgta
    30601 gctacattaa ctggtgctgc tttaacattg ttaggtaatg ttgcaactcc tatagtaaca
    30661 aataatgatg atttttattg tgaattagaa aaagctgcca ctttatcagg ggaaagggta
    30721 tggaagatgc caatttatga tgaatttaaa gatatgataa aaggagaaga agcagacctt
    30781 aaaaacactg gtggtaaaaa tgctggatgt ataacagcag gagcttttat tggtgaattt
    30841 gttgggaata ctccttggat acatatggat atagcgggga caagtacatc atcaaaatca
    30901 attggataca aagcaaaggg ggctacagga gaacctgtta gaacattgta ctatttggca
    30961 gaaatctaaa aaacaattat aaaatataat ttataaagcc acgtggatat ttttgtaatt
    31021 taagattatc tatgtggctt tattttttat ttaagtaaat tatttactct ttttcttttt
    31081 ttcaagtata aaagtatctc tattaaataa aagagcaggt atgtacataa tactagtaac
    31141 aaaaataaac gtaaaaatag cagttgtgct atgacttaaa aaaccataat agtacctaga
    31201 gttggtaaat gtgtttagta aaaatatgaa tgaaaaataa ataaaacata tagctaagtt
    31261 atctctaagt gcatatctgt taactctaaa ttttctaaca tcaaccatat atatatctgc
    31321 ataaattatc aagcacttag agaagataaa gataaaatat attaagctag gaaattcata
    31381 gcccaagttt ttagaaaaaa tgagttctcc aactgaacaa actaaactcc aagaaaaaaa
    31441 tatattaaaa agatgatact gcttaaaaat tagtatggct atacacagat atatacaaat
    31501 ataattgatt ccaattggta aagagtttag agtactataa ttacctgaag aaataagtaa
    31561 taattgctct agagccataa ttattacaag taatgtacaa attatcttct caactgtata
    31621 gctataaggt aaaatatttt tggttagttt tggacaatag tataaaaata atccaaatat
    31681 aaagagactt acaaattgtt cactagaaaa aagaaaaatt ttaaacatat aacattctct
    31741 cctataataa atttaatatg tatctatata aatatattca ttatatctca atttaatata
    31801 taaaaaagat aataatagtt agatttttta tatgaatttt aataggtaaa atatcctatt
    31861 gttttgtaga tatatcctaa ttatgtcaaa aaataaaaaa atttttattt attagagttt
    31921 ttggaaataa taataacaga tattataatt aattaacagt ttttgtaagt atttgacaat
    31981 agagagggtg taacaaatgg gaataataga gtcagcatca aaattggctg agatggttca
    32041 tttattggct gttgaaaagg ggataactga tatagaagca tgggatgaag ctgtgaaaga
    32101 atacagcaaa atttatgaag aaagaagaaa tgaatagaat gataaaaaat gaaaacttga
    32161 tgataaaaaa taactttatt taaagaaatt caatttttat agaagtatta aaaataatat
    32221 aaataaaaag atggtatttc aaatgtaagt aaaaaatcat tttgggatac catccttatt
    32281 atttatttta ataatatata agtaataaat cttacttatc catatttttt atgtaacctc
    32341 ttttttctag cttttttata tgctccttac gtatttcatc aagattaata ccatattgtt
    32401 cttctagctt aaacatcata gtaacacatg tctgcgctac atccattagt tccttagcag
    32461 ttttctttat aatatcaact tcatctaaat cattattttc tccacttaga tttctgaatt
    32521 tacctataca ttcagctaat tcaccttgtt cttcacccat tttgataaat gtagattcta
    32581 gtgttgggtc taaattatta agctctggta aagatatagt ttttagtttc ataaaagctc
    32641 ctttcaagtt attcgtattt atactaaaac atcataacaa aaataatact aaaaaaaaac
    32701 tttaaattca acataaaaac ttacatatat aataaaaatc gtgaaataaa tctatatttg
    32761 tcttaataca gcctcatata aaacttctcc aataggttta tttattacaa gagaagctga
    32821 cttatcatag actgtatttg ctttattaat aagagcaata taatcacctt tgtaataatc
    32881 tataaaactt gctgctgggt acacagctaa tgatgtacct cctataatca ataaatcagc
    32941 attacttata gcagaaattg ttttagtaat gacatcactg tcaagagctt cctcataaag
    33001 tactacatct ggttttacta tagaaccaca gttgtcacag taaggaatat tacctcccag
    33061 atttaacata gattctaagt caaaaaactt accacattta gtacaataat ttctatgtac
    33121 agaaccatga agttctagca catttttact tccagccatt tgatgaagtc catcaatatt
    33181 ttgagttatg acagctttaa gcttacccat ttcttcgagt tttgctaaag ctatatgagc
    33241 attattgggt tttgcattag gatatattaa tttatcctta taaaattcaa aaaattcttc
    33301 tggatatcta acaaaaaaag tatgtgaaac taattgttca gcagtaaatt gtttattgag
    33361 tttttggcta aataatccag tagaacttct aaagtctgga atattagatt cagttgaaac
    33421 acctgctcca ccaaaaaata caatattatt gtgattagct attaagtctt ttaagctatt
    33481 tgcatccata gttacacctc caaattatta tatataaatt gtactacata ttaggtcgtt
    33541 tatgttttat taatttaaat aaatatatag tttaataagt taaataaagg gaatataaat
    33601 aaaatgtata cagagtataa atagacaata tttactaaaa taacaagttg tcgtataatt
    33661 atattaaaat tgttttagtg tataagaagg gcgggggaag tacgtgaaga aaaaagcagc
    33721 tttagcaaca ttggctatgt tacccttagg tgtggtaaat gcacatgctg atggagatat
    33781 aggtatagtg actataaatt atttaaatgt aagaaatgaa ccaactgccg aaagtagcat
    33841 agcctttgtt gctaaaaaag atgataaagt tttgattaaa gactcttcta atggatggta
    33901 taaaataaaa gctgaatctg gacaagaagg ttgggcttca tcaaaatata tagcaaaatc
    33961 aaatagtgac tctttaagaa cgtctacaaa taaagaaaaa caagttattt caaatagttt
    34021 aaatatgaga aatggagcag gtacaagtta cagagtaata actgtactaa aaaaaggtca
    34081 aaaagtagaa gtaatatcag agagtaatgg atggtctaaa atcaaatatg atggaagact
    34141 aggatatgta tctagctctt atttaggaga cgtttcaaac tcaactaata aatcaaaaac
    34201 taaacaagtg aatacaactt cactaaatgt aaggagtgga ccaaatacaa gttatggttt
    34261 gttaggcaag ttgccaaaag gaagtaaagt agaagtaata tcagaaagta atggatggtc
    34321 aaaaataaag tataatggaa aagatgccta tgtatctagt atgtacttat cggatgtaag
    34381 tcaaagtaat tcagacaatt ctagtcaaag caatgataaa aagaatactg ataaggttgt
    34441 aaatacagct tctttaaatg taagaagtgg accaggttct acatatagta agttgggaaa
    34501 agtttataaa ggaagtaaag taactgtact atcagaaagt agtggatggg ctaagattaa
    34561 ttttaacaat aaagaagcat ttgtagtagg caattattta tctacttcag cagatacttc
    34621 aaataataac tcaaatagta actctgataa cagttctaat agtaatggta ataattcttc
    34681 atcatcaggt caagtaaatg gtatgtcagg tataagtggg gctaaaattg attataaatc
    34741 tttgagttat actttagaat ctcatataag taaacaagtt gagaaagcag catcaggagg
    34801 aaatgtgata gctccaagta ataggaaaag tactccaagt cctgaattta gtactttttc
    34861 agctcaaaga acaagctcat ttgtaaatgc aagttcaagt gatatagaat attatttaaa
    34921 tcctaagaat tttacaaata ctactaaagg tatgatgcag tttttaaaga ttaacagtta
    34981 tagagatggt atttcagaat caagtcttaa ctcgtatctt aatggtttat cttctagtgt
    35041 atttaaaaat cagggagcag cttttataaa tgctgccaag aagtataata tagatgttgt
    35101 atatctagta tctcatgcta tgtgggaaac tgcttatggt aagtctacac ttgcccaagg
    35161 tcaaacatta acatcttata agggacaacc tcttagtaag ccagttaaag tatacaattt
    35221 ttttggtata ggagcaatag ataaaagcgc taatgtttca ggtgcagaag cagcgtattc
    35281 caatggctgg actagtgtag aagctacaat agatggctct gcaaagtgga tatcacaaaa
    35341 ttacgtaaat agttctaagt acaatcagaa tactatctac aagatgaagt ggaattatga
    35401 ttatacatgg catcagtatg caactgatgt aaactgggct aatggaattt ctggaattat
    35461 ggaaaattta attggtcttt atggaggagg aagtagtttg gtttttgaag ttcctcaata
    35521 taagtaatac ataaataaaa aatatagact atgggtagtt ttcattttat ttaaattatc
    35581 tatagtcttt ttgtatataa tttataatct gtaaattcaa tgttaacaaa acttttatgt
    35641 tataatatta attctaagta aatataagtg agtaataggt agaaatagat aaaaagtggt
    35701 aataatatat ataaagataa ttttgagaaa ggaggatata tatggaaagt attaaggaat
    35761 atttggataa gttagaaatt aataatagtg gtcttggaaa gcaacttaaa gaagtttata
    35821 taaatagagt agtttatttt aaggaagata agatagttta tttttatcta acttctaaag
    35881 atattgtttc tcatgaactt ttagataaat ttaaagaaga attgatgtat aaactcgatt
    35941 attttaaaga tatgaggatg aagataagat ttactggtct tgaaagaaaa tccaataaag
    36001 atgttataaa aaaatattgg aataatatat tgtatatatt aaagtatctt tgtccatcta
    36061 ttgcagggtg gtataaacaa gtagaatttc tttgtttaga agaagaatta aaaataaaac
    36121 ttccaaaagg tatattttat gagcgattaa tgaaaaaaaa tgtaacttat gtacttaaaa
    36181 ccgtgcttag cgaagagttg ggattagatt taaatataac aatagaaaaa gctgttgatg
    36241 aaaaggtaaa taaagaaaga ctcataagaa tcaatgatag agaaatggaa gaaaaaatta
    36301 gggctttaga aattggaaaa gttaataact gtgaagaaaa tgaagaggaa agctatgtca
    36361 ttaagtctga tgtagatgaa aatttaatct atggagataa tgcaaatgct atgattgaaa
    36421 atatagttga attaaatgca tcttctggaa cagttgcagt tgttggagat atatttgatg
    36481 ttgacactaa agaacttaaa aacggaaaaa tacttatgat agcttcaatt acagattata
    36541 ctagctcaat aagctgtaaa ctttttttaa cagatacaaa taaggatgga gtacttgaaa
    36601 atgttaaaaa aggagcatac ttaaaaatca agggtgacat agtttatgat acatatcaaa
    36661 gagaaattag tatgatgatt agtgggataa gaaaagaaac tagaatagag agagaagata
    36721 aatcagagga aaaaagagta gaactccatg cacatacaca aatgtcttct atggatgcta
    36781 tttgttctgt taaaaagctt gtagagcgtg cagccaaatg gggtcattct gctattgcta
    36841 taacagacca tggtgttgta caaggatttc cagatgctat gaatgcaggt aagtcaaata
    36901 atataaaaat actttatgga gtagaagggt acttggttga agatgattct ctcattatag
    36961 aggatgcaaa tgataaagaa ctttcacaga catttgttgt atttgacata gagacaactg
    37021 gtttttctaa tacaaatgat aaaataactg agattggtgc tgtaaaaata gaaaacttta
    37081 agattgtaga tagatttagt gagttaataa atcctgaaaa agatatttct tataagatac
    37141 aggaattgac aggaataact aatgaattaa ttaaagacaa gccaaccatt gaagaagttc
    37201 ttcctaagtt tatggaattt gttggagata gtgtattagt agcacataat gcagagtttg
    37261 atactggatt tatatcgcaa aaatgtaggg aacaaggctt gttatataat aacaaaaaag
    37321 tagatacact tatgttagca agagtaatgt tgccaaactt aaaaagacat agattaaatg
    37381 tcgttgcaaa agaacttggt attcctcttt taaatcacca tagagcagtg gatgatgctg
    37441 aagctacagc acttatattt aataagtttt tgcagatgct tacagaaaaa ggtgcaaaaa
    37501 ctctaagtga tgtaaacaat atacttggaa agattgatta cacaaagctt agcacaaatc
    37561 atataacctt gatagcaaaa aattctatag gtataaaaaa tttatataaa ataatttcag
    37621 atgcacatgt aaatcatttt tttagagcac caagaatcct tagaagtgta ttaaatgaat
    37681 ataaagaagg attaataatt ggttcggctt gtgaagctgg ggtggttttt caagctgtaa
    37741 agaaaaacgt gtctgatgag gaaatgaaaa aaataataga tttatacgat tatattgaag
    37801 taatgcctat agataataat agatttatga ttgataaagg agaagtaaag gacgaagaag
    37861 agttaaggga gttgaatagg aaattaattg atacagccaa aaaatttgac aaaattccag
    37921 tagctactgg ggatgttcat tttttagaca aacatgaagc tgtcttaaga aaagttctta
    37981 aatattctca aggttttaaa gtagatgaag aagaaactta tttacatttt agaacaacag
    38041 atgaaatgtt ggaagagttt agttatcttg gagaagaatt ggcatatgaa gttgtagttg
    38101 aaaatagtaa tctgattgcc gatatggttg aagatataaa acctatacca gatgatactt
    38161 atcctccaat tatagagggc tcagatgtgg agttaagaga aatgtgttac aaaaaagcca
    38221 aacgaatcta tggagaccca ataccagaaa tagttcaaaa aagactggat agagaattaa
    38281 attcaataat aagcaatggg tatgcagtta tgtatattat agctcaaaaa ctggttgcaa
    38341 aatctctttc agatggatat ttagttggtt caagaggctc agttggctca tcttttgcag
    38401 ctactatgag tgatataaca gaagttaatc ctcttccagc acattatatt tgtgaaaacg
    38461 aagactgtaa atattcattc ttttatgaaa taggtgaatg gggctcaggt gttgatttac
    38521 cagacaagga ttgtccaaag tgtggaagaa aacttgctaa aaacggtcat gatattccat
    38581 ttgaggtttt cttaggtttt gaaggtgata aagaaccaga tatagactta aatttctcag
    38641 gaaattatca gccaacaatt cataaataca cagaagaact atttggagag gggtatgtat
    38701 atagggctgg gactataggt actgtagcag aaaaaacagc ttttggatac gcaagaaaat
    38761 atgtggaaga aaataatata agtgtaccaa atgcagaagt acttaggctt tcaaatggat
    38821 gtactggagt aaaaagaacc tcaggacaac atcctggtgg agttatggtt ataccagact
    38881 ataaagatgt atacgatttt acacctatac agtatccagc aaatgatact tcttgtggtg
    38941 ttataactac tcactttgat tatcattcaa taagtggtag aatacttaaa cttgatatac
    39001 ttggacacga cggtcctacc attataagga tgcttgaaga tatgactgga ataaatataa
    39061 cagaaattcc tttagatgat aaagagacta tgtctctatt tacatctaca gaagcattag
    39121 gtgttacacc agaagaaata aactgtccta taggttgcct tgctgtacca gagtttggaa
    39181 ctaagtttgt acgacaaatg ttacttgaca caaaacctac tacatttgca gaacttgtac
    39241 gtatatctgg tctctctcat gggacagatg tttgggtaaa taatgcacaa gacttagtta
    39301 gagaagatat agttggatta aaagatgtta tatctacacg agatgacata atgaactatt
    39361 taatatttaa aggattacct cccaaaatga gctttacaat aatggagagt gtaagaaaag
    39421 gtaaaggttt aaaaccagaa cacattgaag aaatgaaaaa aaataatgtt ccagaatggt
    39481 atataggctc atgtaaaaag attaagtaca tgttcccaaa agcacatgct gttgcttatg
    39541 taatgacatc atttagaatt gcatattgta aagttcacta tccagaagca ttttatgcga
    39601 cttattttac gacaaaagtt gaggattttg atgcagattt aattgttaaa ggattagatg
    39661 ctataaaatc aaaaataaat gaaattgagt cactgggaaa tgatgcaacc acaaaagaaa
    39721 aaggtatgtt gaccgtttta gaagttgctc ttgaaatgta tgctagagga ataaaattat
    39781 taccagttga tatatataaa tcagatgcaa ctgagtttat agttgtagga gaaaaaacat
    39841 tacttccacc aatggcagca atacaaggtc ttggaggaaa tgcggctata aacatccaaa
    39901 atgaaagaaa aaatggtgaa tttatatcaa aagaagattt gagaaagaga acaaaaatat
    39961 caaaaacggt aatagaaact cttactaatc atggttcact tgagaatatg agtgagagaa
    40021 atcaattgtc attgttttaa gttagcccaa aaagttgcaa ataaaagaat tttatgatat
    40081 catagtatag aaaatagtat actttgcaga aactaagagt gagacagctg ttctcactct
    40141 tttattttaa atatattatt aaaaagctgg tttattttta ttaaatatat tttatttaag
    40201 attatataaa ctggtcattt acaattgaat aggaggaact ttaatgaaaa aaaatttaga
    40261 agctacaatt gaggagattg taactaaaat aacggatgaa catggatttg aaatggttga
    40321 tgttgaatat gtaaaagaag caggagaata ctacttaaga gtatacatag ataaagaaga
    40381 aggaatatct ttaaatgaat gtgaattagt tagtagagaa ttaagtccaa tactagatga
    40441 aaaagaccct ataaaagaaa attacttttt agaggtatct tctccagggc ttgatagagc
    40501 attaaaaaaa gacagagatt ttgttagata tcaaggtaga gatgtagatt taaaattata
    40561 taaaccatta aatggatgta agcaatttga aggtgaactt gtaggattaa cagaagataa
    40621 taatataaaa atcattgcaa atggtaagga aatggaattt aatagaaaag atgttgctat
    40681 tgtcagatta gcaataaagt tttagtggta tattaaatag ggaggatata aaaaatgaat
    40741 catgaattta tggaagcatt agatgaatta gtagttgaca gaggtataga taaagaaata
    40801 cttatagata ctatagaaca agctctttta actgcataca agaaaaactt tggttcagca
    40861 caaaatgtta gagttgaatt tgatagagac aagggagata taaaagtatt ttctcaaaga
    40921 gttgttgttg atgaatcaga tttatatgat actttcttag aaatagagtt agcagatgct
    40981 agagagataa accctaatta tgaattaggt gatattatag aacatgaagt tacacctaaa
    41041 aactttggaa gaattgcagc tcaaacagca aagcaagtag ttgttcaaag aataagggaa
    41101 gctgagagag aaatagtgta taatgaattt atagaaaaag aaaatgaaat agttacagga
    41161 gaggttgcta gagcaaataa aaatgttgtt tatgtaaatc ttggaagaat agaaggtata
    41221 atgactcaaa gtgaacaaat accatttgag aattataaag caggtcaaaa aataaaattc
    41281 tatatacttg aagttaaaaa gactaacaaa ggacctcaaa tagttgtatc aagaagtcat
    41341 ccgggtctag taaagagatt gtttgaagaa gaagttcctg aaatatatga aggaatagtt
    41401 caaataaaat ctgtagcaag agaagcaggg tcaagaacta aaatggcagt aagatctata
    41461 gatgaaaaaa tagacccaat tggagcttgt gttggaccaa aaggacttag agtaaaaaat
    41521 atagtggatg aacttggcga tgagaaaata gatataataa aatatagtga agacccagca
    41581 gagtttatat cagcagcact tagcccatca aaggttgtta gagtagatat agatgaagat
    41641 gaaaagtctg ccttagtaat cgttccagat tatcagttat cgcttgctat aggtaaagaa
    41701 ggtcaaaatg caagactagc agcaaaattg actaactgga aaatagatat taagagcgaa
    41761 tctcaagcag aacaagaaat gttaaatctt gaaaattcca aagaaataga agctgaggat
    41821 gttgaagctg aatttatcga ccaagaagag ttagatactt cagaggataa ttttagtgaa
    41881 gaagagtaag gagggatgat tttgaaaaaa gtaaaaaaag tgcctcaaag aaaatgcata
    41941 gcatgtcaag atagagactc taaaaaagag cttataagaa tagttaaaaa taaagaagga
    42001 aaaatatttt tagacccaac aggaagagcc aatggaagag gagcatatgt atgtaaaagc
    42061 tctgaatgtc taaaaaaagc tataaagagt aaagcattaa ataaagcttt taaaatagat
    42121 gttcctgatg aagtctatga taatctactt atggagttag aagaatatga aaaataataa
    42181 agaaaaagta ctttcgttct tagggctcgc aactcgtgca gggaaaatag tgtctggtga
    42241 tgattcaact ttgcttgacc ttaaaaaagg aaaagtaaat ttgattttaa tcgcagaaga
    42301 tgcatcaaat aatactaaaa aactttttaa agataaatca acttttagaa atattccata
    42361 tctatttttt tcaacaaaag aggaaatagg atttgcaata ggaaaatctc caagagcagt
    42421 tgtaggaata aaagatgaaa atttttctaa aaagataatt gaactaatag aaatttaaaa
    42481 taaaataggg ggtgatcttg tgtcaaaaac aagagtatac caaatagcag aagaattaaa
    42541 tatatcaaat gaagagctta taaataagtt agctgaatta gatataaatg taactgacaa
    42601 agattcagta ttagaaggtg aagagttaga attagcactt gaaatgttag gtgaagattt
    42661 atctcaagaa aatggaaatg ttatagaaat agatggaaag ttgacagtac aagtattagc
    42721 aacgaaatta gataaatctc catcagaaat aattatgaag cttatgaaaa tgggaactat
    42781 ggcaacaata aatcaagaga taagttttga gattgctgct ttggctgcca aagactatgg
    42841 atttgaatta acagtagctg aaagtgatga tacagaagct ttagaaatag aagctttaat
    42901 ggaaattgaa gaagataaag aagaggattt aaaacctaga ccaccagttg ttacagttat
    42961 gggacatgtt gaccatggta agacatcttt actagatgca ataagaaaaa ctgatgtgat
    43021 ttcaggtgaa gctggaggaa taactcagca tataggtgct tcagaagtta aaatcaatgg
    43081 acacaaaata gtatttttag atactccagg acatgaggct ttcacatcaa tgagagcaag
    43141 aggagcacaa gttacagata tagcaatatt agtagttgca gcagatgatg gtataatgcc
    43201 tcaaacagtt gaagctataa accatgctaa agcagcaggt gtacctttaa tagttgctat
    43261 taataaaata gataaaccag gtgcaaaccc agataaggtt aaacaggaat tagcagacca
    43321 aggattatta gttgaagact ggggtggaga agtaatagca gttccagtat cagctaagaa
    43381 aaaagaagga atagatacac ttttagaaat ggtattattg gttgctgaaa tggaagagtt
    43441 aagagctaat ccaaataaaa gagcagtagg aactgttata gaagctgagc ttgataaagg
    43501 aagagggcca gttgcaacag tacttgttca aggtggtact ttaacagtag gtgacccaat
    43561 cgttgcaggt gttgcatgtg gtaaagttag agcaatgata aatgcaaaag gtaagagagt
    43621 aaaaacagca ggaccatcta cagcagtcga gatattaggt ctatcagaag ttccacaagg
    43681 aggagaccaa tttgtcgaag ttccaactga taagattgct agaagtgtag cagcaagaag
    43741 acaacaaata gttagagatg aaatgttaaa atcaactcaa agattatcac tagatgctct
    43801 atttagtcaa atgagtgaag gttctataaa agaccttaat atagttataa aagcagatgt
    43861 acaaggttca gtacaggcag ttaaacaatc tttagaaaaa ttatcaaatg aagaagttca
    43921 agttaaagta atacatggtg gtgttggagc tgtaacagaa tcagacatat tacttgcagc
    43981 agcttctaac gcaataataa taggattcaa tgtaagacca gttccaggtg cagaatctct
    44041 aggagaaaaa gaaaatgttg atataagaac atatactata atatacaagg caatagaaga
    44101 tatacaagcg gctatgactg gtatgttaga ccctgaatat gttgatgaag aaacaggaaa
    44161 agctgaaata agagaaatat ataaaatatc tggtgttgga acagtagctg gatgttatgt
    44221 gactaatggt aagatattta gaaattgtaa agtaagatta gttagagata gcataataat
    44281 acatgaaggt gagcttgcag cacttaagag atttaaagat gatgtaaaag aagttaattc
    44341 tggatacgaa tgtggtatga gttttgtaaa ttataacgac ataaaagaag gcgatatagt
    44401 agaagcttat ataactaaag aagtagaaag aaaattataa attcgtatca aagaaaatca
    44461 gttagtaaag aaggtcgatg gtatggcatc atataataga acaagaagga tagcagaaga
    44521 aataagaaaa gtcgtaagca ctatgcttat aaatggagta aaagacccta gaataacatc
    44581 tatggtgagt gtaactgatg tagaagttac aaatgaccta agatatgctt atgtatatgt
    44641 aagcattcta ggtggagatg aagaatccac actaacaggt cttaaaagtg ctggaggata
    44701 tataagaaga gaagtaggaa aaaatataaa acttagatat attcctgaaa tagtatttaa
    44761 acttgatgat tcaatagaaa aaggaatgta tatggatagc cttataaaaa gagttaatga
    44821 aaaaaatgct caacaaaatg aggatgaaaa ttatgatgaa taatatggat agtattataa
    44881 actatattta tgagggtgac tattttatag ttacctctca tatcagtcca gatggagaca
    44941 atattggttc tacactttca atgtattata cattgaagaa tttaggaaaa aatgtatatt
    45001 atgtacttga tgatgaagct ccattaaatt taaggttttt ggttgaagat gtaaaaatct
    45061 ataagtctaa tgaatttaat atgaagaact attctctaat tgcacttgat tgtggggata
    45121 agatgagaat atgtgtgagt gatgaaatca aaaataatgc atcaaaatta atctgtattg
    45181 accatcatgc tagtaatgat tcatatggag atttaaatta tgtagatatt gatgcatctt
    45241 ctacatgtga attagtttat aatttattag ttagatttca gcaaataaaa gatattaata
    45301 ttataaatga agatatagca acatgtttat atacaggtct tgtaactgat actggaaact
    45361 tttcgtattc aaatgtacat gcaagtagct ttgaaatggc aaaaaattta cttgttttag
    45421 gtgcacaaaa aaatactata atacaaaata tatatcaaag caattcttct ggttattaca
    45481 aattattagg agaagctcta aagggtttgg agatatttga ttcaaaggta tctagtatag
    45541 ttttaactca agacatgatg aatagaaata atattagctt taatgatgtt gatggaataa
    45601 caacatatac aagagacata aaaggtatag aagttggaat tttatttaaa gagaaaaaac
    45661 aaaatgaaat taaagtgagt tttaggtcaa aaaattatgt tgatgtaagt gaaatagcca
    45721 agttatttgg tggtggtggt catgtaaggg ctgctggatg tactattaga gatagtattg
    45781 ataatgcaaa gaaaatggta ctagaggcag tgttaaaatc aatttaaggt gacagtaaaa
    45841 tgaataagat tataagtata ttgaaaccca caggtatgac atctcatgat gttgtaagta
    45901 gagtgagaaa gattttaaat ataaaaaaag ttggtcatac aggtactctt gacccagatg
    45961 cttcaggtgt tttaccaatc tgtataggga aagctacaaa agtatgtgaa gttattttaa
    46021 acaaagataa atcgtatata tgtgagctta ctttaggtat aagtacagat acttatgatg
    46081 cttcaggtga aattttaaaa aaagttgatg attttaaatt tagtaatgaa gacatagaaa
    46141 gagcttttga cacacaaaga ggagagataa atcaacttcc tccaatttat tcagctttaa
    46201 aggtaaatgg aaagagaatg tgtgatttag taagaagtgg tagacaatct gaaattactt
    46261 taaaaacaag aagagtaaat ataaaagaca taaaaatact aagtataaaa ggtaataaag
    46321 taatgtttta tgttgagtgt tctaaaggga cttatgtgag aagtatttgc catgatatag
    46381 gagaatatct aggttgtgga gctcatatgt cgtttttgaa tagaacatct tctggtaaat
    46441 ttgacttaga taattcaata acattagaag aattagaatt attttatgaa aataaaactt
    46501 tagacaaata cttatatgat atagattatg tccttgattc gtttaattat gttgtattaa
    46561 accctaatgc tataaaatat tatagtaatg gtggctcaat agatgataaa aggtttttaa
    46621 agaataattt cgataaagat gatgaatttg taagagtata cagtacagat aatttcttag
    46681 gattaggaaa gttatcaaaa cacaataata caataagtgt taaaagtgat aagatgttca
    46741 tataaccaat gtatttaaaa aaacaacaaa agagatagta ctataataac ttctatagtc
    46801 tatctctttt acttatgcta taatagtttg tgaataagtt aagagtaaat tatattttag
    46861 cggggaacat ttattatggt gattataaaa tctataaaag aaatagcaaa cataaaagaa
    46921 agtgtcatta ccataggaaa ttttgatggt gtacacaaag gacatcaagt tcttatagga
    46981 aaaacagttg aacatgcaaa aaaagaaaat atacagagta ttgtatttac atttgcaaat
    47041 catcctgtaa attactttag accaaactca acaaaaaata ttataagcaa tgatgataaa
    47101 ataaaattat tagaagattt gggaatagat atagttgtaa tcataccatt tgatgagtat
    47161 atgactaaaa tacctgcaaa ggatttcgta gatgagatac tagtaaaaaa attaaaagct
    47221 aaaaagatag taattggaca tgactttaca tttgcaaggg caaaagaagg caatgttaat
    47281 ctattaaaaa gcttagaaca tgaatttggt tttgaggtag aagtaataaa acccatcaaa
    47341 atcaatgatg taagagtgag tagtacttat attagaagtt tagtatccca gggtgatatg
    47401 gctaatgtca aggaatattt gggtagaaat tacaagttag aaggttgtgt tatacattct
    47461 aaacaccttg gaagaactat aggattccca acggcaaata ttgaccttaa gaataatatg
    47521 ctcgttccaa agagaggtat ttatgcttct attgtacata ttggagatga gacttatttt
    47581 ggtgctacaa atgtaggcta taacccaaca gttaatggaa aaactttgtc tattgaaaca
    47641 aatattttag agtttgatag agatatttat ggggaaaata taattgttga atttttggaa
    47701 agaataagag atgaaagaaa gtttaattca atagatgact taaaaaatca attgtataat
    47761 gatacaaatt atgtgtatga aaactatgtt tgcaaaaaca agtaatgtat gataaaatga
    47821 ttaaggaaaa taaccattac tcggcatttg aatactccaa tcaatgctta gtaataggcg
    47881 tttctaaaaa aatacggagg taatctaaat gataaacaaa tcagaaataa taaaagaata
    47941 tggaagagct gaaggggata ctggttctcc agaagtacaa atagctttat taacagctag
    48001 aataaacgag ttaaatggac atttaaaaac tcataagaaa gaccaccatt caagaagagg
    48061 actattaaaa atggttggta gaagaagaaa cttattagct tacttaaagg aaaaagatct
    48121 tgaaggatat agagcattaa tagctaaatt aggattaaga aagtaatttc attctttata
    48181 aaaaagcagg tatagtgtat acctgctttt ttatagttaa ttcattataa agtaaaatag
    48241 ttaataataa aagtagttta tatgttataa aaaattataa aatgaataat attgggtaag
    48301 aatattaaaa tagtatacaa atacataatc aatttaaaag gaaagacagg ataaatatag
    48361 aaattttata acaggataat tagttggaat aaggaggata ttattttgaa aataaagtta
    48421 gtatgcgata gtttgtgcga tattccagat gaaatatcag aaaaagatta tatagagata
    48481 gttccactta cagttatttt taatgatagg gaatatatag aaggagtaga tataaagaaa
    48541 gaagagtttt ataaaaaagt aaaagaaatc aagcaaatac ctaaaacttc acaagctaca
    48601 tatatggagt ttaaagaagt gtttgataag ttcataacag aaggttatca tataatctgt
    48661 atgacagggg ctgcaaacgc atcaggaact tttcaaagtg ctatgatagc taaaaatgat
    48721 gtaaatgaaa atgaaaaaat acatatattt gatactagaa atttatctct aggtagtgga
    48781 caatatgtta taaaggcatg tgaacttctt gaagaaggac tgggatttga agaaattata
    48841 gatgaattag aaaatactcg cagtagtgta agattgcttt ttgcaccata tacacttgat
    48901 tttttaaagc agagtggaag agttccagtt gcaactgctt tgataggaaa tatgttaaat
    48961 ataaagccta tatttttctt tgataatggg gaagctaagt tggtaaataa ggttagagga
    49021 ataaagaata tagcaagtaa attggtcgat ataattttag aaatgaatga agggtcttta
    49081 gaaggaaaaa tcgtcacaat aggttgtgga gataatttac acgattgtga aatattaaaa
    49141 gatgaagtaa ataaaagaat aaaagcaaga agagtattat ttactcgagg aggagtttct
    49201 atttgctctc atacagggcc agatatattg gctataagtt gttctaatta gtagtaatat
    49261 aaggtttttg catatatatg tgaagataat tttatattgt taatttatag tatttttgat
    49321 aatatagtaa atagagtgat atacgtgttt tttaataaga ggaggacaag cattaatgtt
    49381 tgaacataaa atatttaaaa tggattttgc aggaagagaa ctttctgtag aaataggaaa
    49441 gatttgtgaa atggctagtg gtagttgtat agttcgatac agtgatagta tggtaatggt
    49501 aaatacaact aaatcagcca aacctagaga cggaatagac ttttttcctt taagtgtgga
    49561 ttatgaagaa aaattatact ctgtaggtaa gatacctgga ggttttttaa agagagaagg
    49621 caagccttca gagaaagcta tacttacatc aagattgata gatagaccaa taagaccttt
    49681 attcccaaaa ggatttagaa atgatgttca ggtagttgca acagttctat cagtagacca
    49741 agattgtaca ccagatatag tagcaatgat aggttcttca atagcccttt ctatatcaga
    49801 tataccattt aatggaccaa caggttcagt atgtgttggc cttgtagatg gtgcatttgt
    49861 tgtaaatcca aacgcagagc aaagagaaaa aagttcaatg catctagttg tttcaggtac
    49921 aaaggaagca ataatgatgg ttgaagccgg agctgatgaa gttccagacg aggtaatgct
    49981 tgatgctatt ttatttgctc accaagaaat aaaaaaaata gttgagttta tagaaggtat
    50041 agttgcagaa gttggaaaag aaaaaatgcc tgttgaatta taccatgcag gagaagaaat
    50101 aactcaattg gttcgcgaat ttgcaactga taagatgaaa aaagcagttc agacttttga
    50161 aaagttagag agaatggaaa atatggatag agttaaagaa gaaactttag ctcactttga
    50221 agaaacttta gtagattttg aagattttgt tggagatata gaagaagtat tacaagatat
    50281 aataaaagaa gaagtaagaa aacttatagt acatgaaaat gtaagaccag ataatagaaa
    50341 acttgaagaa ataagaccta tatggtgtga aactggcatg atacctagag ctcatggttc
    50401 agccatattt actagaggac aaactcaagt acttaatgtt gctactttag gtgctttagg
    50461 agatgttcaa aaactagatg ggttagatga agaagaaaac aagagatata tgcatcatta
    50521 taatttccct gcatatagtg ttggagaagc tagaccatca agaggaccag gaagaagaga
    50581 aataggacat ggtgctttag cagaaagagc tttattacca gtaataccat ctcaagaaga
    50641 atttccttat gctatcagat tagtttctga agtattaagt tcaaatggtt ctacatctca
    50701 agcaagtgta tgtggctcaa cattatcttt acttgatgca ggtgtaccta taaaagatat
    50761 ggttgcaggt atagcaatgg gacttataaa gcatgatggt aaggttgcag tgctttcaga
    50821 tatacaaggt atggaagacc atttaggcga tatggacttt aaagtagctg gaacagagta
    50881 tggtataaca gcaatacaaa tggatataaa aattgatggt atagataaag aaattctaca
    50941 acgtgcttta aaacaagcta aagagggtag aatccatata ctaggagaga tgagaaaaac
    51001 tatatctcag ccaaaaccag aactttcacc atatgcacct aagattgtta aaatgcaaat
    51061 aaatcctgac aagataaaag atgtaatagg acctggtgga aagataataa ctaagataat
    51121 agacgaaact ggagttaaga tagatattga acaaactggg gaagtattta tatcaggtat
    51181 tgagattgat atgattaaga aagctcaaga acttataaat aatatagtag ttgaaccaga
    51241 agtaggaaaa acttataaag gtaaggtaag tagaataatg aactttggag catttgttga
    51301 gatacttcca ggaaaagaag gattacttca tatatctcat atagctcatg aaagagttgc
    51361 caaagttgaa gatgtattga atatagggga tgaagttgaa gttaaagtta cagagattga
    51421 tgaaaaagga agagttaatt tatcaagaaa agttctttta ccaaaaccag aacataaaaa
    51481 taaataactt attatataat taaaagcaaa gtcttaggat tttgcttttt taaatacaaa
    51541 aaagtagata attttaaaat gatagttatg gatggcttat agttcttttt ctgtcaggct
    51601 ttactcttat aaagaagcta tttaaaagct gattaataga gagctttatt gatatatata
    51661 gaatcactta agttatattt agtgcataaa cagagcttat ttttaatagt attaaataag
    51721 aaacaatttg tacataatta gggggagttt taatgataat gatggtactt aataagagta
    51781 agctcaaaaa aatcataata ctaattttag tagtgttatt agtattatca ggaggggtaa
    51841 tggtattaaa gttatttaac aacaagccag tatttaattt atttaatggt gtggatttac
    51901 catatgaaag aggtacaaag gataaaagtg gatatgttgc attaacctgt aatatagacc
    51961 ttggatggga aacagagtac gtagaatcca ttttagaaac acttaaaaaa gaaaatgtaa
    52021 agattacatt taatgtaact ggaaaatggg cagaaaagaa taaggatgaa ttattaaaga
    52081 taaaaaaaca aggacatgaa ataggtaatc atggatacaa acatttagat tattctacat
    52141 tatcatatga agacaattat gagcaaatag agacctctaa aaagataatt gaagaaatta
    52201 taggtgaaaa aactaaattt tttcaagcac cagcaggttc ttttggtcca gaaacagtaa
    52261 aggctgctaa agcattaggg tatacttcta tcaaatggga tgccgatact atagattgga
    52321 aatacaaaga ccaaccagag gtaatcattg atagaatgaa gaaaaaagac attaaagata
    52381 gtagtataat tttaatgcat ccaaccaatg caacgacaaa atgtatagat gatattatag
    52441 ctattgtaag agaaaaagga ctaaaaccag gaaaacttag tgatgtattt aagtagacaa
    52501 agtataaata tttggttaag attgtataag ttgtagttga attaattaaa agactgactt
    52561 ataatgtaat acatataaaa aataaatata attaaaacat agtgttttca ataatccaat
    52621 atgaaacaaa gaataaattg ttaggagaat gtaatgtata agacaaaaat actggaaaat
    52681 gggcttacaa taattggtga agaaatacca tatctaaagt caataacatt aggtatttgg
    52741 ataaatgcag gctctaggat agaagaagca caggtgagtg gaacttctca ttttatagag
    52801 catatgatgt ttaaaggaac caaaaacaga acatcaaaag agattgctag ttcaatagat
    52861 aatttgggtg gtcaaataaa tgcatttaca agtaaagaat gtacatgtta ttatgtaaaa
    52921 ctaatagatg agcatataga tacaggaatt gatgtattga gtgatatgat actaaattct
    52981 aaatttgata aaaatgatat agataaggaa agacttataa tattggaaga acttaagatg
    53041 tatgaagact ctccagatga cttatcttat gatttattag tggaaaatat atatgctaat
    53101 gatggattag gaatgaatat aattgggaca aaagaatctc tgtacaatat aactagagaa
    53161 tccatgttag aatacttaaa taagtattat attccaaata acgctgttat ttctatagct
    53221 ggaaacttta actttgatga tatggtggag aaaatcaaat caaaatttgg acactgggaa
    53281 aagaaaaatt taagtataga cataagtgag gctaagttta atccttgctt tatatcaaaa
    53341 aataaagata ctgaacaggt gaacttagct atgtgcctaa aaggtattcc ttttgaaaat
    53401 gatgaagaag tctattcaat ggcagtggta aataatattt tcggtggaag tataagctca
    53461 aggctttttc agaaaataag ggaagaaaaa ggtttagtat attcaatata ctcatcacag
    53521 acattatata ggaaatgtgg tgaattaggt atatttgcaa gcatgagtac agaaaattta
    53581 caagatgtat ataatttaat aaagaaagaa attgaaaata taagagaaaa ctatcttaca
    53641 gaaaaagaaa tatctgaaag caaggaacag ttaaaaggaa attatatatt ggatttagaa
    53701 agtactagca gtagaatgat gtctactggt aaatctatgt tacttagtaa aaaagtaaaa
    53761 actacagatg aaatacttga atgtataaat aatgttaata ttaattcaat aaagaaggtt
    53821 gttgataagg tattcaatat cgaaaatatt ggtacatgta tagttggaag agatgtagaa
    53881 aagattctac acttggatta atattgaaaa aagtaaatct ctggaggtat aaaatatgaa
    53941 tttatctgaa atagcaggta aggaaatagt taacttggtc acaggagaaa gattgggggt
    54001 cattggagaa tgtgacctta tactagatga aactacaggc ggtatattag cgcttctaat
    54061 tccaaaagaa aaaggatttt ttgccagaag aaaagataag tcatttttag aagtaccatg
    54121 gagaaatgta aaaaaaatag gtaatgacat gataattata gaatatgagg gatatattta
    54181 ggaggaaaaa atgagtattt tagtgcaaaa gtttggtggg acatcagtag aatcatatga
    54241 aaaaatgaat gaagtatgta agatagtaaa agcatacaaa aaaaatgatg aagaattaca
    54301 attagtatta gtagtttcag caatgggaag aaaaggagct ccatatgcta cagatacact
    54361 tataaatttg tgtagtgcag taaatgatga gccaagtaaa agagaacttg atttaataat
    54421 gtcttgtgga gaaataatat caggaacaat tcttgctaat ttattgaatg cacaggggat
    54481 agattctgtt ttctttactg gtcagcaagc aggtataatt acaagtgatg aatattctaa
    54541 tgcaaagata aagtatataa atcctaaaaa ggtaaaaaga gctttagatg atggtaaggt
    54601 tgtagttata gctgggttcc agggtgtaac agatgatgga gaaataacta ctcttggaag
    54661 aggcggaagc gatacatcag cagttgctat tggtaaagct ttagaatgtg aaacagttga
    54721 aatttacact gatgtggatg gtataatgac tgcagaccct agagtagaac caaacgctaa
    54781 agtattgagt tttatagatt atgaggaagt attccaaatg gctgacaaag gtgcaaaagt
    54841 aatacatcca agagcagtac agttggctaa aagtggaaat ataactttag ccataaagaa
    54901 tacaatgaat ccaacttttg aaggtacaaa aataggttct ttatgtagac acttagaaga
    54961 caatatagaa tatgaacaag aaagagattt taaggttgct gttgcaaata aagatagcgt
    55021 agcacaagtt aagattaagt cagcagaaga agtgtttaca gaagtattaa atgagataga
    55081 aaagaaactt ataactatag atatgataaa tttctttata tcagaaaaag catttgttgt
    55141 agaagatgct gatataaaat cattaaagga aatactagat aaatttgaat tagattatga
    55201 agtaaataga gactgtgcta aggtaacatt gatatgttca agaattgctg atgaaatgtc
    55261 tggtatcatg tcaaaggtag ttagaggatt atcaaaagca ggggtatctc ttttacaaac
    55321 atcagactca aatatgacta tatcttgttt agttagtgaa gaagatatgc atacagcagt
    55381 acatgctata caccaacaat tttatttaaa ataaactgta taatacaatt attttttaat
    55441 caaatttaag aatttcatat atatcttaat atatttgatt tatttggtaa aactaaatta
    55501 aatataattt tgataggagt ttttttatga aagattatag agaagataat gaaagatttg
    55561 ataaagataa ttgtagtgaa gaatctagtt gcgaagagag agaatgtata aagcaatttg
    55621 gtaccaatga aatgccacct caacctccta aagatattca atgtataact ataattggag
    55681 aaattgaggg acattttata ggaaatcctc aaaaaaaagc tacaaagtac gagcatataa
    55741 ttccaatgct atactcaatt gaggaaagta atgatgttaa aggagtgtta gttgttttaa
    55801 acactgttgg aggagatatt gaagcaggtt tagctattgc agaactttta aacagtacat
    55861 ctaaaaaagt agttactctt gttctaggag gaagtcatag tataggtgtt cctcttgcta
    55921 cagcaggaga ttactcattt atagcaccaa cagcaaccat gattatacat ccagtgcgaa
    55981 ctactggtct tgtgattggg ataaatgaga cttttgagta ttttaaaaag atgcaagata
    56041 gaataattca atttataata agaacatcaa atatcaaaaa agatgtactt gaaaaactta
    56101 tgcatgaaaa agatgaactt gtaagtgatg ttggtagtgt attaatagga aaagaagctg
    56161 tggattatgg tcttatagat gaagttggag gactaaaaga agcacttaaa aaattaagag
    56221 agcttataaa agaatctgag gaagaaaaaa ataatgatta aaatttgttt aaataataaa
    56281 atttatgata taatgatgta ataaagtagg agtgttagtg ttaaggcatt agcacttttt
    56341 gctgtgaagg ggtaaaactt gctaaaaata aggagtgata ttaatggcca cgaaaaagaa
    56401 gaaaaaaaag aaggtaaata ccttaagttt taatgcagaa tatcataatt taataacaat
    56461 atttataggt gtatttttat tatatagttt aaattctaat tctatgggat ggatacctgt
    56521 gttaatgcaa aatcttttta agggactatt tggagggctt tcaatagcaa taccatttat
    56581 agtaattata acaggacttt taggattttt tgatggaaat gaatatatat ataggctaag
    56641 aaagactaaa ctatactaca taattatttt atttatattt gtattttatg gattattaaa
    56701 tgcaggaact cttcctgtag atagcccact aaaaggtaac atgtttgatg atgttatgaa
    56761 attgggtgta agtggacaag gtagtggttt gatagcaaca actattgcgt attatatgag
    56821 taaaatattt ggtatagcag gtggatggtt aataagtatc ttcgcactta tactttctgt
    56881 aatgtttata tttaatattt ctataaaaga tttggtgtca aatgcaaaat caaaatctaa
    56941 agcatctaaa gacagcaatt taacgtttaa agataaaatt gcaaatatga aaaaatctgc
    57001 aatagatatg atgactgatg aagtagatga tactactata aataaaaaac caggtttctt
    57061 taaagggtta atgtcaaaag gtcttaataa tgatgatgaa gaagatgaat attttgaaga
    57121 agaaaatagt gatggtgtag atgataaaac tataaaaata gttggattta ataaggcaga
    57181 agatgagtat ttagaaatat tagaaggaac tcaaagtatg ccagagttag atgttttaaa
    57241 agaattacaa aaagctacca atgaaaatcc agttgtagat actaaaccag aaaaaaaagt
    57301 tgatatagct aaacctaatt taaatataga aaaaactcaa cctatgagta ttgtagcaga
    57361 gccagttaat gaggattatt caaactataa aaagcctagc attgagcttt taaataaagt
    57421 aaataagaag tctgatgaaa atggtaaaaa gaaagtgttg aaaaatgctt ctcttttaga
    57481 aaagacacta tcagattttg gagtggaagc aaaaattaat caagttacag ttggacctac
    57541 tataacaaga tatgaaatac aaccaagtcc aggagtcaag gtaagtaaaa ttgtaaattt
    57601 aactgatgat atagctttaa gtttagcagc taaaagtata agaattgaag ccccaattcc
    57661 tggtaaaagt gcaattggaa tagaagttcc aaatgaagaa gctcaaatgg ttggtgtgag
    57721 agaagttcta gaaagtgaag aatttaataa ttttaactct cctcttgcaa tgggacttgg
    57781 aaaagacgta gcaggaaaaa ttataattgg agatataggt aagatgccac acttactaat
    57841 agctggttct acaggttcag gaaagagtgt gtgtgtaaat acattaataa gtagtatttt
    57901 atataaggca aatcctgatg aagtaaaatt actattaata gacccaaaag tagttgaact
    57961 tgcaaattat aatggaatac cacatctatt gataccagta gttactgacc ctaaaaaagc
    58021 tgcaaatgct ttaaattggg cagttacaga gatgaataga aggtacaaat tatttgcaga
    58081 tgctcaagta aaagatgtaa caagttataa tgagaaagcg gaagaaaaac ttcctaaaat
    58141 agtaataata atagatgagt tagcagattt aatgatggca agtgcaaatg atgtagaaga
    58201 ttatatttgt agattagctc aaatggcaag agctgctgga atgcacttga ttgtagcaac
    58261 tcaaagacca tctgttgacg ttataacagg ggtaataaag gctaatatac catctagaat
    58321 agcatttgca gtatcatctc aaactgattc aagaacaatc cttgatatgg gtggtgcaga
    58381 gaagcttcta ggtaaaggtg atatgttatt ctatccactt ggtgcagcaa aaccagttag
    58441 acttcaaggg gcattcatat cagagagtga gtcggaaaaa gtaatcgatt ttgtaaaaag
    58501 tcaagttaaa gatggaataa aatatgaaga agatataatt gaaactatat ctaaggttaa
    58561 tacttcaaag ggtagtgatg aggatgagtt tttgagtgaa gcgatagaat ttgtcgtgga
    58621 aagtggtcaa gcatctgcct ctatgctaca gagaagattt aaaataggat tcaatagagc
    58681 agctagactt atagattcta tggaagaaag aggaataatt ggagcaagtg agggaagtaa
    58741 accgagaaag gtattaataa gcaaacagga tttgcagaat ttagaaggtg aatagaattg
    58801 tcagttataa aaatagaaaa agcacttaag ctagataaag taatatttgt agatgtgaga
    58861 actgaaggag aatatgaaga agaccatata ttgaatgcaa tcaatatgcc tctttttaaa
    58921 aataatgaac ataatgacgt tggaacaatt tataagatgc agggaaaaca tgaagcaata
    58981 caaaaagggt ttgactatgt atcgtataaa ttaaaggata tatatttaca agcagcagaa
    59041 cttgcattaa attatgataa tattgtcata tattgtgcaa gaggcggaat gagaagtggc
    59101 agtatagtaa atttattatc atcattaggt gtaaatgtat atcaacttga aggtggatat
    59161 aaggcttata gaaattttgt attagagtat ctaaggcatg taatggatac aaaaaacttt
    59221 gtatctgttc atggattgac tggtacagga aagactgatt tgttacattt attagatgaa
    59281 aaaggaatag atgtactgga tttagaaggc atagctaaaa acagtggttc tacctttggt
    59341 tttataactt ttgataaaaa gccaccttct caaaagaact ttgaaacaaa actatttgaa
    59401 agtatatttt tttcgaaaga gaattatata tttgtcgaga gtgaaagcaa acgagttgga
    59461 agtgttacag ttccaaatga aattttcgag gcaatgacac gagaaggaca tcatatatta
    59521 ttagaatgtg atattgaaaa tagagttgat agactttgta gagattatat ttatgataaa
    59581 gatgaaggta atatattgat tttaaaagaa tgtataaaca agtttagaaa aagattgggt
    59641 aataaagtag tagatgatta tattgattta ttagaatcag gtgaatataa agaacttgta
    59701 aaaagatatc ttttagaata ctacgaccca ctttatatgc attcagtaga aaaatataag
    59761 tatagtaaga ttataaattt taatgataca caaaaagctt tagaagatgt tatagactta
    59821 tatgagagta tattggaagg agaaagtgaa tgttaaaaat agcattagaa tcattaggat
    59881 gctcaaaaaa tttagtagat gcagaaataa tgatgggaat tttaaataac aaaggctata
    59941 aattaattgg agattttgaa gaagctgatg taattatagt aaatacatgt ggatttatag
    60001 agtcagctaa acaagaatct atagatacga taataaactt tgcagagtta aaaaaaactg
    60061 gcaatttaaa gttgttgata gtaacagggt gtctagccca aagatattct gaagaattaa
    60121 aaactgaaat tcctgaaata gatgctatag taggaacagg aagctatcaa aatatagata
    60181 aaatacttaa agaattaagt gaaatgcata aaattgtaag ccttaatgat atagagtttg
    60241 tatttaatga agatttacca agatatatat ctacacctag ctatatggcc tatttaaaaa
    60301 ttggagaagg atgttccaat aattgtactt attgtataat accaaaacta agaggtaaat
    60361 atagaagtag aaaatttgaa gatataatta aagaagctaa gaaattagct gaatctggag
    60421 taaaagaact tgtggttata gcacaggata cgacaaagta cggttttgat ttatatggaa
    60481 aagaaagatt atcagaactt ttagaagaac ttgcaaaaat agatggattt aagtggataa
    60541 gggttatgta ttcatatcca gaatctataa cagaagaatt gatacaagtt ataaagaaat
    60601 atgataatat atgtagttac tttgatatgc caatacagca tgcaagtaac aatattttaa
    60661 aattaatgaa tagaaaaact actaaagaag atatattaaa taaaatcaat ctgataagaa
    60721 gtaatatacc agatgctata cttagaacta caataatagt agggtttcct ggtgaaacag
    60781 aagatgactt caaacaactt gtggactttg ttgaagaggt taaatttgat agacttggtg
    60841 catttgcata ttcaagagaa gaagatacac cagcagatag attgccaaac catatagatg
    60901 aagaagtaaa aatacaaaga agagatactt taatgatgat tcaacaaaaa atatctgaag
    60961 aacttaatga caaaaagata ggaaaaactt atgaggtgtt aatagaagaa cagatagaag
    61021 ataatgtata tacaggaaga acacaaggtg atgcagaaga aattgacagt atagtgtatg
    61081 taaaaagtgt agacaatctt gaagtaggag aatttgtaag tgttcaaata aatgatgcaa
    61141 tggaatacga tttaatggga gatgttttat atgaacttgc ctaacaagtt aactttgttt
    61201 agaatatttt taataccagt atttgtttta attatgttgc tcaatatacc aaataaattt
    61261 ttaatagctt gtattatttt tataatagct tcaattactg atgctttaga tggaaaaata
    61321 gctagaaaat acaatctagt cactgacttt ggaaaattta tggacccatt agcagataag
    61381 ttattggtta tttcagcact tacatgtatg atagaagacc atcttgtaag tagttggatg
    61441 gtaattataa tagtggctag agagcttaca gttagcatat taagagctat agctgctgct
    61501 gatggaaaag taattgctgc tggaaacagt ggaaaactta aaacgataac tcaaatggta
    61561 tctatagtgt ttttattgtt aggtgctcag tttgaaaatg tattgatatt aaatattggt
    61621 gaaattctca ttcttatagc tacactactt acattatatt ctggatggga atatctatat
    61681 aaaaataaag agctttttat gtcttctaag taaaaaatta tgctttaata aataaaaact
    61741 aatgattaat aaataaaaaa taacaactta aaattttaaa aatgtaaaaa agggtatact
    61801 attatagtat acctcttttc attttaagaa tattttcttt tgtgtatata attattaata
    61861 atggtttata aataaaatgt aatgaataaa aatataatca ttagtagcgt ttttaaattg
    61921 actaggtttg atttatgtta tataattaat atataacaag agaacaaatg tttgtagata
    61981 aatgaaggga tggtatagag atgagtgtag atcaagaaaa attaaaagcg ttgaatgaag
    62041 ctttaggtaa aattgaaaaa gattttggta aaggttcagt aatgaaattg ggagaagcaa
    62101 catctatgtc tatagatgtt atatcaacag gagcgattgg tttagacata gctattggta
    62161 taggaggtct acctagaggg agaatagttg aagtatatgg tccagaatct tctggtaaga
    62221 ctactgttgc gcttagttgt gtagcatcag ctcaaaaaga tggaggaata gctgcattta
    62281 tagatgcaga acatgcactt gacccagtat atgcaaaagc tttgggtgtg gatgttgata
    62341 acctaataat atctcaacca gatacaggtg aacaggcttt agagatagca gaggcattga
    62401 taagaagtgg agcgatagat ataatagtaa tagactcagt agcagcatta gttccaaagg
    62461 ctgaaataga tggagatatg ggtgattctc atgtaggttt acaagctaga cttatgtcac
    62521 aagcacttag aaagttaact ggttcaatta aaaaatcaaa ttgtgttgct atatttataa
    62581 accagttaag agagaaagta ggaataatgt ttggaaaccc agaaactact actggaggac
    62641 gtgcactaaa attctattca tcagttagat tggatgttag aaaaatagat acaataaaac
    62701 aaggtgataa agttataggt agtagaacta gagttaaagt tgttaaaaac aaagtagcac
    62761 caccatttaa gcaagctgaa tttgatataa tgtatggaga agggatttca aaaattggag
    62821 accttttaga tatagctgct gatgtagata tagttaaaaa atcaggttca tggtatagtt
    62881 acaatgatac taaacttgga caaggaagag aaaatgttaa aaaattcttg gaagataatt
    62941 tagatttaac tactgaaata gatgagaaag ttagagcatt ttacaattta aatgaagaac
    63001 atgaagaatc aggtacttca gtatcaaaag aaattgtaga agaataagta aaaatacaaa
    63061 aaaataacta gtaattaaag acatgccacg ctccctatta gcttgacaca atgttgaaaa
    63121 aattataaaa ttaagtgagg ataaaatatc taaatttaat tttaattatt aggaatattt
    63181 tttaatcata gtgatgacat tttgggatta ttaacgaagg aggtggatgt catagatagt
    63241 gtagtaatga ttgtaatagg tgcagctgtt ggtattatag ctggatattt tgttagaaaa
    63301 aatatatctg aagcaaaaat tggacaagct gaaaacttgg ccaaagaaat aatagataaa
    63361 gcacaccacg actcagaaac agtacaaaaa gaaaaattac tagaagctaa agaagagatc
    63421 cataagtgga gaacagaagc tgaaagagaa aacagagaaa gaagaacaga agtacaaaag
    63481 tatgaaaaaa gagtagtaca aaaagaagaa gtcttagata gaaaattaca aaatttagaa
    63541 tctaaagaag taaacctaag tgaaaaatta aaggtagtgg aaaagaaaga agaagaagtt
    63601 gaagtaataa aaactcaaca gttagaaaaa ttagaaagta tatcaggaat tacttctgat
    63661 aaggccaaag aaatcatctt aaccaatgcg gagagagatg taagacgtga aatgtctata
    63721 atgataaaag agatagaaag tcaagctaaa gaagaagctg ataagaaatc aagagaaatt
    63781 ataggatatg caattcaaaa atgtgcagca gatcacgtag ccgaaactac tgtaacagta
    63841 gtaagcttgc ctaatgatga aatgaaaggt agaataatag gtagggaagg tagaaatata
    63901 aggactttag aaaccttaac aggtatagac cttataatag atgacactcc agaagccgta
    63961 attttatcag gatttgatcc aataagaaga gaaattgcaa gaattgctct agaaaaatta
    64021 atagcagatg gaagaataca tccagctaga attgaagaaa tggttgaaaa ggcgagaaaa
    64081 gaagttgaca atattataaa agaatatggt gaacaagctg catttgaaac tagtgtacat
    64141 gggattcatc cggaattaat aagattatta ggtagactta actatagaac tagttatgga
    64201 caaaatgtac ttaaacattc tatagaagtt gctcatatag ctggtataat ggctgctgaa
    64261 ataggagcag atattagatt ggctaagagg gcaggtttat tacatgatat aggtaaggct
    64321 gtagaccatg aaatggaagg tacacatgtt gaaataggta tggatttact tagacgttat
    64381 aaagagtcta aagaagttat acatgccatg agtactcatc atggagatta cgagcctcaa
    64441 acagtagagg ctgtattagt tacagcagca gatgcaatat cagctgctag acctggtgcg
    64501 agaagagaaa ctttagaagc atatataaag agacttgaaa aattagaaga aatagctaat
    64561 tcatatgaag gtgtagaaaa atcatttgct atacaagctg gtagagaaat aagaataatg
    64621 gtaaaaccag aagctataaa tgatgaagaa atacatctat tggctagaga tatgactaag
    64681 aaaatagagg atgaacttga atatcctgga caaataaaag taagcataat aagagaaaca
    64741 agagctatag agtatgcgaa gtaaaagaaa taatatctaa ataataagaa aatattttta
    64801 gaaatttaaa tttgccacaa gatatgataa tatatcttgt ggcttttaaa ttgtttaagt
    64861 ttaattttat ataatgcaaa aataatctaa gtgctaaaag tgctacttac aaaagattaa
    64921 taaaataaag ctttatatat gtattattta tataattatc caagtttgtt tgatataatg
    64981 aagttgtaaa ataaatatat tactacttgg aatgcttgta cttaaataag atgaaaatac
    65041 aatgacgcta aagtgaggat tagagagagg gtacactatg aaaaaaaata cacttttaca
    65101 tacaaatatt atggtttgca ttattattac tcttggtttt gtggctacct ctataattgg
    65161 atatcaatct aataccaata tttttaaaaa tgatattgaa catgtatcta cattggctgc
    65221 tgaaggtatt tattatcaga tagataaact actttcagaa cctataaatg tatctcttac
    65281 aatggcaaat gacagtctat taaaaaattt tttagatggt gagaaagaac atttaaatga
    65341 tgaagaattt atttataaat tacaagatta tctgaatgtg tatagaaata aatattcata
    65401 tgattctgtt tttttagtat caactaaaac caataattat tatcatttta atggacttga
    65461 tagaaatctt agcgctaata actcagagaa tcaatggtat tatactttct taaagaatga
    65521 tgatgaatac tcattaaatg ttgataatga tgaagcaagt aacaatagta ttacagtctt
    65581 tgtaaattgt aagataaaag atgataatgg agcaactatg ggaattatag gtgttgggtt
    65641 aaaggttaat tcattacaaa tgttgcttaa aggatataat gacaaatttg atgttgttgc
    65701 acgcttaatt gatgataaag ggtttgtaca attagcagta gataaaaccg gtcatgagaa
    65761 tataaatttt tttgaaaatt ctagtagcga cttgtctaat tcgaaacaat taatattaaa
    65821 caacaaaaag gaacaaaaga gtttttggta ttcatctgaa aaatcaaaaa gttatattgt
    65881 atgtcagtac atacctagtt taaaatggca tcttgttcta gaaaatgatt ttactttaat
    65941 gattaaacag cttcatctgc aattttttaa aggcattgca gttatagttt taattataat
    66001 atttgtactt tttactatta cacatgtgct taaaaaatac aaaaaccaaa taataaagct
    66061 tagtgtttca cagaaattgg aatatcaaaa attgttgagc aaatctactg aaggtcttta
    66121 tgaaaatatt tttgaaattg atattactaa caataaagct ggttgtgaag atgagaatag
    66181 gtattttcat gaatttggat tagggcttaa taccccatat gatgaagcct tacatacaat
    66241 agctaaaaac caaatcaaag aagaatatgc acaaggttat ttagatacat ttttacctga
    66301 aaatgtaatt aaacgttata atagcggaat taataatatc agctatgatt ttttaattaa
    66361 aaaagatgaa gaaagttatc attggattag aattaatgca cgaattttct tttggacatc
    66421 agatgaatct gttcgtatga tatcttatcg taaaaatata gatgaagaaa aaaatagaga
    66481 attactactt ctcgaacgtt cacaacgtga ttctcttaca gggctttaca ataaagctac
    66541 aacagaggaa tttattcgtt catttttaaa aaatgaaagt aatcaagatt tagaacacgt
    66601 ttttctaatt ttcgatattg ataattttaa aaatgtaaat gataaatatg gtcatgtatt
    66661 tggagacttt gttataagtg aatttgcctt agaattaaaa tctcaatttc gtgaggacga
    66721 tattgtggga cgtattggtg gagatgagtt tgtagtatta ataaaagact ttaataataa
    66781 taactatgtg cttatagaca aattagagcg tttttgttct aagattaatc agaaaaaatt
    66841 tgttgagcaa gcagaatttt ctattgcatg tagtattggt gtagcaatgt atcctgaaca
    66901 tggatatgta tactctgaac tatatgagaa agcagaccaa gcactttact atgcaaaaag
    66961 ccatgggaaa aattcttatc atatattttc agaggattct attgatagta tttcatttca
    67021 tatagactct agagatataa aagtattatt agaaatggct actgatggtg ttgtcaaatt
    67081 tgcttgtatg gaaaacttaa aattacttta tttcaaccaa aaaatgcttg aatttactgg
    67141 atattcagct caaactgtta cttctatagg ttttgatatt ttttcgcctg tattacaaga
    67201 cgacttacaa atggtactag atacattaag agaagcaatt tctaaaagag agacttttga
    67261 aatagttttt cgtttttgtc atacagatgg tcatattttt cctgtaagga tgcaaggtaa
    67321 gtttgtaagt gaattatatc aaaatcgtta tcctgttttt tatgcgattt atacagacat
    67381 atcaagtatc aatactttac tagaaaataa atagtaaata ctacagtaaa atctaaaaat
    67441 aggatataat agtattaata taagaagatt ataaataaaa cctgctaatg aaaatagatt
    67501 atttttagca ggtttttaaa ttttttataa taaatcttat ttttggctaa caagatttat
    67561 tttttttaag actttactta ttatt
//
LOCUS       DAGSRM010000011        64303 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.011, whole genome shotgun sequence.
ACCESSION   DAGSRM010000011 DAGSRM010000000
VERSION     DAGSRM010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 64303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64303
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.011"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(359..871)
                     /locus_tag="MJE33_000792"
     CDS             complement(359..871)
                     /locus_tag="MJE33_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBY9646760.1"
                     /translation="MLEVIKNRHSIRTYIDKNIEEDKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKQLSKSQHRAKHIEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWAGVFPKNKVVNKVRQTLDLPVKLVPYALISIGYSEEKNEFIDRFDEN
                     KIHRNVYKNR"
     gene            1321..1797
                     /gene="purE"
                     /locus_tag="MJE33_000793"
     CDS             1321..1797
                     /gene="purE"
                     /locus_tag="MJE33_000793"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBY9646761.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     SIFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDKNLRG"
     gene            1803..2501
                     /locus_tag="MJE33_000794"
     CDS             1803..2501
                     /locus_tag="MJE33_000794"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBY9646762.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            2513..3880
                     /gene="purF"
                     /locus_tag="MJE33_000795"
     CDS             2513..3880
                     /gene="purF"
                     /locus_tag="MJE33_000795"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBY9646763.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            3874..4950
                     /locus_tag="MJE33_000796"
     CDS             3874..4950
                     /locus_tag="MJE33_000796"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBY9646764.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVGGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMEQYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHDIKAIAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVDERELHKSFNMGIGLVLIVDKENADDVVNFINNRENDNAAYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            4944..5537
                     /locus_tag="MJE33_000797"
     CDS             4944..5537
                     /locus_tag="MJE33_000797"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBY9646765.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQGAYGL
                     ERAKNHNIKAICETDEDKIIEILKENKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKLKLQGRRVFINE"
     gene            5530..7062
                     /gene="purH"
                     /locus_tag="MJE33_000798"
     CDS             5530..7062
                     /gene="purH"
                     /locus_tag="MJE33_000798"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBY9646766.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEILKQKKNLRILKLAKIENSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKEKP
                     TDAQLKDMEFGMRVVKNMKSNAIAIVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            7073..8323
                     /gene="purD"
                     /locus_tag="MJE33_000799"
     CDS             7073..8323
                     /gene="purD"
                     /locus_tag="MJE33_000799"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBY9646767.1"
                     /translation="MKILVVGGGGREHAICWKLSKEKNVEKIYCAPGNAGIANVAECV
                     NIGDTNIEELLKFAKENEIGLTIVGPEVPLVMGIVDEFEKEGLRVFGPNKKCAQLEGS
                     KAFSKEFMIKHNIPTAKYKEYTNLEEAISEIDSFGYPVVIKADGLAAGKGVVIPENRE
                     DAIATLKEMMSDKKFGAAGDKIVIEEFLKGIETSILAFVDNDTIVPMASAKDHKKVNN
                     YEQGPNTGGMGTFSPSEIYTEELANKVKETVLEKTLEGFKKDGLNFKGILFVGLMITE
                     DGEKVLEYNVRFGDPETQSVLFRLETDLHEIMEAILDNKLKDIEINYSDDEAVCVMLT
                     SGGYPDSYEKGKIITGLENLDDDIVVFHSGTKMFDGNLVTNGGRVIGITAKSTTVKDA
                     AEKVYENIKKINFEGMHYRTDIGR"
     gene            8348..12154
                     /locus_tag="MJE33_000800"
     CDS             8348..12154
                     /locus_tag="MJE33_000800"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HBY9646768.1"
                     /translation="MLNTENKDSMVRRVLVEKREGFDLEAKALKKDLVESLHIDNIEN
                     LRILNRYDVEGISEEVYENAAKTIFSEPNLDVVYYEEIPKLNDERVFAIEFLPGQYDQ
                     RGDWAAQCVQIVNQGIRPAINTAKVYILSGKITDEEFSKIKDYCINPVDSREASLEKP
                     ETLKMETEIPTTVEVLDGFIDLDENGLRTFVSEKGLAMTLGDLQHVQKYFKDTEKRNP
                     TITEIKVLDTYWSDHCRHTTFMTEIENVKIEDGKFNDIVKEAYQMYLNSRDNVYVNRH
                     KDICLMDIATVAVKELKKNGKLNDLDESEEINACSINVDVEVDGKMEKYLVMFKNETH
                     NHPTEIEPFGGAATCLGGAIRDPLSGRSYVYQAMRVTGSADPRTTLEDTLPGKLMQRK
                     ITTEAAHGYSSYGNQIGLTTGQVAEVYDENFVAKRMEIGAVIAAAPKENVVRERPEAG
                     DVIVLLGGKTGRDGCGGATGSSKEHSEESILTCSAEVQKGDAPNERKIQRFFRNKEVA
                     QMIKRCNDFGAGGVCVAIGEIADSLDINLDLVPKKYDGLDGTELAISESQERMAVAIK
                     KENKDKFIQLAVEENLEATHVATVTDTGYLRMFWNGKAIVDINREFLDTNGVKQTTDV
                     HVTKVDEENTFFSSNEIVKDVKCASMKDKFTKVLSDLNVCSQKGLVEMFDNTIGGNTV
                     LMPFGGKYQATPTQGMVAKIPVLGGETNTSTIMTYGYNPKVGKWSPFHGALYAVVESV
                     CKLVAIGGNYSTTRLTFQEYFEKLGNNPEKWGKPFSALLGAFYAQSKFEIPAIGGKDS
                     MSGTFKDIEVPPTLVSFAVDTVDAKKVVSPEFKKADSKVVMLCVNKAENDVVDFEELK
                     RNLDKVRELIHGNKVLSTYALGFAGVGEAISKMAFGNKIGFKFSEEAEKAFTDDKLFE
                     ASYGNIVLELANDDLSMLEGYNYVVLGSTVKEASIFIKGEELALDELYKAHCSTLEPI
                     FPTKTEEVKSKIETISYISQGEAKKSSLSIATPRVFIPAFPGTNCEYDSARAFERAGA
                     NASIRVFKNLTYKDIEDSIDTIVNEIKSSQIIMLPGGFSAGDEPDGSGKFIATVFRNP
                     RVQEAINEFLTQKDGLMLGICNGFQVLIKLGLVPYGEIRVPSESAPTLTYNNIGRHQA
                     KIARTRISSNKSPWLAQTNVGDIHNIAISHGEGKFVASEDVMRELIANGQVATQYVDF
                     NNEATYDIEFNPNGSFYAVEGITSVDGRVFGKMGHSERIGEEVYKNIIGEKDQKIFES
                     GVKYFR"
     gene            12360..13232
                     /gene="rfbD"
                     /locus_tag="MJE33_000801"
     CDS             12360..13232
                     /gene="rfbD"
                     /locus_tag="MJE33_000801"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBY9646769.1"
                     /translation="MKILITGSNGQLGKELVNQLEAINQSINQPKYVILATTRSDLDI
                     SNQTNVDNFILHNKPDVVVNCAAYTKVDACEDNIEIAYKINALGVRNLAIASEKVNAK
                     LIHISTDYVFNGFSKYPYREDNKTEPNSVYGKSKLMGEKFVEQFSHKYFILRTAWLYG
                     NGNNFVKTMIKLSLENTEVNVVNDQFGTPTSTVDLAKIIIKIMETEHYGVYHATCEGE
                     CSWYDFAKKIFKLKNIDIKVNPIKSNEFKCKAPRPLYSVLDNFMLKLIGLNSFRKWEE
                     SIEEYLERYDINEK"
     gene            13341..14210
                     /gene="rfbA"
                     /locus_tag="MJE33_000802"
     CDS             13341..14210
                     /gene="rfbA"
                     /locus_tag="MJE33_000802"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBY9646770.1"
                     /translation="MKGIILAGGSGTRLYPITKCISKQTLPIYDKPMIYYPMSVLMLA
                     GIQDILIISTPRDIKAFEELFGDGSSLGLNITYAIQEKANGIAEAFIIGEEFIGNDRV
                     SLVLGDNIFYGYGFSERLENAVKRKGATIFGYHVSNPNSFGVVEFDKDFNVLSIEEKP
                     TVPKSNYAIPGLYFYDNDVVEIAKLVEPSDRGELEITSINSEYLKRGKLKVELLGRGM
                     AWLDTGTHKGLLDASNYVEVVQTRQGLYIACLEEIAYTKGYISREQLMKLAKPLIKTE
                     YGQYLLKLASEDM"
     gene            14259..14816
                     /gene="rfbC"
                     /locus_tag="MJE33_000803"
     CDS             14259..14816
                     /gene="rfbC"
                     /locus_tag="MJE33_000803"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBY9646771.1"
                     /translation="MGKINVIKTKIEGLYIVEPNVFYDERGYFMESYNKKDFIEQGLD
                     MCFIQDNESSSRKGVLRGMHFQVKYPQGKLVRVTEGEVFDVAVDLRKDSQTFGKWEGV
                     ILSDKNKKQFYIPEGFAHGFLVLSEKAVFSYKCTNYYYPEFENGFIWNDKQISIEWPL
                     KGIEEIFLSEKDKKQKSFKDICTRR"
     gene            14854..15837
                     /gene="rfbB"
                     /locus_tag="MJE33_000804"
     CDS             14854..15837
                     /gene="rfbB"
                     /locus_tag="MJE33_000804"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBY9646772.1"
                     /translation="MKKILITGGAGFIGSNFIHYMLNKYVDYFIVNLDLLTYAGNLET
                     LIEIQDNSNYKFVKGDISNREQVYKLFEEERFDIVVNFAAESHVDRSVENPDLFIKTN
                     IIGTQVLLDASIKYGVKRYHQISTDEVYGDLPIDRKDLFFTEQSSINPSSPYSASKAS
                     ADLLVSSYYRTYGLLTTISRCSNNYGPYHFPEKLIPLMILNALENKQLPVYGNGENVR
                     DWLHVYDHCTAIDLIIHKGNIGEIYNIGGHNERSNLEVVKMILNLLGKSEELISYVND
                     RPGHDLRYAIDATKIENELGWKAKYDFDLGIKETVKWYIENESWWKAVLSV"
     gene            15867..16535
                     /locus_tag="MJE33_000805"
     CDS             15867..16535
                     /locus_tag="MJE33_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBY9646773.1"
                     /translation="MNEISSLINILALTQLSGSNTTNQCNCTYNNSSGFDMIMMTLLK
                     ALSQNNQHTSNSYNLLSNSENLFNELDNVVNNTTSRFIDVDTKDKNVKLRIENAVDQA
                     SKKYNVDSNLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTDPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMKSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
     gene            16544..16972
                     /locus_tag="MJE33_000806"
     CDS             16544..16972
                     /locus_tag="MJE33_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HBY9646774.1"
                     /translation="MGINIDKLNISPSINMEVKKLKESNKFSDKLNSLKQSSSLEDIK
                     RNLESVKKAGERLVLTQNYGDISIYKNAVKEYLKSVVDNMYSLDKNSSFWEHQYYKNV
                     KIVDSKLEDMTSKLISEEKENIDIVSTVDMIQGLLIDMYR"
     gene            16996..17310
                     /locus_tag="MJE33_000807"
     CDS             16996..17310
                     /locus_tag="MJE33_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBY9646775.1"
                     /translation="MNSKKEDIYELNFGQLIEEKSGVIEENQNVFNAKVNVAVSIGNT
                     TETIKNILDLKEGSIIKLSKNVDEKLDIYANDRLFAYGESIITNERLSVRLSKIEDPD
                     VN"
     gene            17462..17737
                     /gene="flgM"
                     /locus_tag="MJE33_000808"
     CDS             17462..17737
                     /gene="flgM"
                     /locus_tag="MJE33_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HBY9646776.1"
                     /translation="MDIKNVSSNIVVNNYLKNTSGIKKVEEVKKINKTDKIELSKFAK
                     EMASVSNMSDENIRNKKVDEIKNALENGTYKIKPKEIAKKMIEDMRG"
     gene            17742..18158
                     /locus_tag="MJE33_000809"
     CDS             17742..18158
                     /locus_tag="MJE33_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HBY9646777.1"
                     /translation="MITDLKVIIYEEKNIISKMMELLKEQFDYIVEKDLENLNMLNPK
                     LEDISRELASIEIKRRQLFGDDISMSEVVENSNDGYLKEIYTDLKHILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPADKQTTTYNSYGNIRK"
     gene            18176..19486
                     /gene="flgK"
                     /locus_tag="MJE33_000810"
     CDS             18176..19486
                     /gene="flgK"
                     /locus_tag="MJE33_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HBY9646778.1"
                     /translation="MAGLFSILNTANSGMNAHQKAIQTISHNISNLDTDGYSRQRTEF
                     ATNSPMYMPSLSNSIARGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGKTVSKYDYY
                     NTIETILNEPSDYGISAGIDDFFKGWESLSKDINSDSKKSLVVENASNLATLISESYK
                     KLDKLKEDIDSNIDTEIKQINDMLKSLEDLNKSIDIISGSGSTPNDLLDERDRILDNL
                     SFKLDFENSDVKNMLADGKLELNELKNPDGTWKTGISGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNTSAGITVTDFFITSNVDGEDIIKINPAIKSNPNELKLTADEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAESIGLDSQKVNQDEANQRKIINNIDNSR
                     MSVSGVSLDEEMTELIKIQRSYQASAKVMSTAVQLLDVVINGII"
     gene            19505..20437
                     /gene="flgL"
                     /locus_tag="MJE33_000811"
     CDS             19505..20437
                     /gene="flgL"
                     /locus_tag="MJE33_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HBY9646779.1"
                     /translation="MRVSTGMMSSSYLNSLQDNLQRLDKVNRQVNTTKEINKLSDNPY
                     KAIKILNSKSEIKTMETYVENCKDTGNWLETTDTALDQLGKLLSDIKKTLVASGDGAY
                     SDDELKTLSNETNEKMKEIANALNATHEGKYIFSGSNTGVPPVECIDNADGSVSLKFN
                     SSLNLNKLNDSLSMDIAQGISVDYNVKLSSLGFDPTKTDPFEELNNISKKLANPDADA
                     IKELTTTCLGDMEQLIENTVNVRSIYGTKANTVEAMKEKNDDSLIQLKDILSQNEEID
                     YGEKLVQLKAAELTYQASLQTGGKLFNVSILDYI"
     gene            20453..20845
                     /locus_tag="MJE33_000812"
     CDS             20453..20845
                     /locus_tag="MJE33_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HBY9646780.1"
                     /translation="MMKVTLKKGILGFENLKEYELLDIEDNDSLKEFNSTEEEGIGFV
                     VVSPFEIINEYEIVLNQETIEKLEVKSPKDVVLFNIITLGQTFEESTVNMKAPIVINI
                     KTNFGMQIILQDDKYSIRHPLLRGDNGC"
     gene            20839..21051
                     /gene="csrA"
                     /locus_tag="MJE33_000813"
     CDS             20839..21051
                     /gene="csrA"
                     /locus_tag="MJE33_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HBY9646781.1"
                     /translation="MLVISRKKDEAVLIGESIEVKIVGVDGNNVKLAISAPNNISILR
                     KEIYEKVKSENIKATNKNIKILKSLK"
     gene            21073..21468
                     /gene="fliS"
                     /locus_tag="MJE33_000814"
     CDS             21073..21468
                     /gene="fliS"
                     /locus_tag="MJE33_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBY9646782.1"
                     /translation="MYGENPYNSYKQNAVFMASKEQLLLMLVDGAVKYTKIARAAILD
                     KNTQKAHRELVRVQDIFTELMVTLDQNAGQWAKDMYKVYDFVRYELSRANIRKDIQVI
                     DSVLPVIEQIKDTWHEADKKSKEERSRDK"
     gene            21468..21824
                     /gene="fliS"
                     /locus_tag="MJE33_000815"
     CDS             21468..21824
                     /gene="fliS"
                     /locus_tag="MJE33_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBY9646783.1"
                     /translation="MELNLAELDKLSKEELLLMLVDGTVKYTNISKEALLNNDYLKAH
                     NELVRVQNIFTELMTTLDQNAGQWAKDMYKVYEFIKSELAIADENKDIKIIDDILPIV
                     KQIRDTWHEVYNQLKI"
     gene            21845..23368
                     /gene="fliD"
                     /locus_tag="MJE33_000816"
     CDS             21845..23368
                     /gene="fliD"
                     /locus_tag="MJE33_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBY9646784.1"
                     /translation="MSSISPIRVTGLSGNFDMEGIIEASMIRDKEKVDKAKQEQQIVK
                     WKQEIYRNVIQESKDLYDKYLSVNSPNSIVSEKAYSSTRITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKFTIKLNSNEPIVRQFPPNASGASSLTIGDVNIPISEQDTTSTI
                     VSKINSLCADNDIKATYSEMTGELIISRKQTGSSSDINLRVIGNDNLAQQIANDNGIT
                     FINDAGGNKVASVYGKNLEADVTDEHGRVTHISKEQNSFNIDNIDYNVNSKGTAKLTS
                     ITDTEEAVKNMQAFVDDYNKLMDKVYGLVTTKKPKDYPPLTDAQKEDMTTEEIEKWEK
                     KAKEGILRNDDELRGFVEDIQSAFFGDGKNIIALRKLGINESENYNKKGQISFNADTF
                     SKALIDDSDKVYKTLAGYSSNYDDKGMFEKLKDIVYEYSGSSTSKLPKKAGIEKTASA
                     SENVYSKQIAEQERNISRLVEKMNDKEKRLYAKYSALESLLNQYSSQMNYFSQAQGN"
     gene            23371..23706
                     /gene="fliT"
                     /locus_tag="MJE33_000817"
     CDS             23371..23706
                     /gene="fliT"
                     /locus_tag="MJE33_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HBY9646785.1"
                     /translation="MDKLKEKLNLYKDISLQIINLIEKEEYIKISIKLGERQEIINSV
                     SEIDRNDFIQLYNRMELIEIDSRIRDILQGQLLEVKKELHEYKLTKQVNTMYYNLNRE
                     KVNIFNKKV"
     gene            23808..24680
                     /locus_tag="MJE33_000818"
     CDS             23808..24680
                     /locus_tag="MJE33_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HBY9646786.1"
                     /translation="MRVNTNVSALIANNQMGRNVNAQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGALEETGNILQRMRTLSVQSSNETN
                     TAEERQKIADELLQLKDEVERISSSIEFNGKKLLDGSSTEIRLQVGANFGTNVAGTSN
                     NNNEIKVALVNTSSIMSKAGITSSTIASLNADGTSGTNAAKQMVSSLDVALKELNTSR
                     AKLGAQQNRLESTQNNLNNTIENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            24773..26914
                     /locus_tag="MJE33_000819"
     CDS             24773..26914
                     /locus_tag="MJE33_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBY9646787.1"
                     /translation="MLLSIVMIVKNEEKILEKTLKSLTTLRNSIESELIIVDTGSTDD
                     TIKISKKYTEKVYFHNWNNDFSSMRNISISYAKGEWLLILDADEILIDSSTIVKFFKD
                     GLDKKFNSASIQLKNLYSYDKKLYGYCSVLRLFKNVDFRYHGKVHEQPIYKNPIFNNV
                     ADFEHYGYLFEDEEIRLNKVKRNEKLLFQELKENDNSPYTNYQLGKNFIILGKYQEAL
                     YYLEKSYELYSKLELAPGYLITSLVKTYLYLGKHKKCERLCLKYIKKDMNNIDIYYYI
                     AQAQVDLGKYENSIDSYKRYIYLLDNYEISTQANSLFSDTDTVGLRDEAIITLIKIYY
                     KLEKYEFVISEYNNIEDVEKKKNVYFSLFMSLYKLNRFEDIKNYYKEVSNSKIEKNSF
                     YRNIEEVINNIKEDEKEYVYKILCDIEGNYGILNQIRINKCVSIDKCKEILNEEKEII
                     YAYIIKIAFEEKIDLLDIFYEMEYVWIEKYLKYLLALERSFNLVLYKYIISRPNTSDM
                     EKIRVYKAISKVVLESISLSDEKYKEIFYFYIMYSYKYIRYIYSNLSDFELKKFVCND
                     LDKFTIEFKHLLDIKNKVNNANEKLEYIHSMRKLLNEYPFYNKIIKILIGELEENIKE
                     SEEFKDLKYSFIISIENMIETGKINDAKLLVDDYSKTFVDDVRILNIKGILYMFENKL
                     DKADFMLKKALSLDVENEDTIYNIEYLKSLR"
     gene            26938..28815
                     /locus_tag="MJE33_000820"
     CDS             26938..28815
                     /locus_tag="MJE33_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBY9646788.1"
                     /translation="MNIPIVVNEKSKDIIKDWQYTCTNEDIKFTSIIILTYNQIEYTK
                     LCIESIRKFTPKNKYEIIIIDNNSSDGTVEWLKGQSDIKSVYNNKNLGFPKGCNQGIE
                     LATGENILLLNNDVIVTPNWLYNLNKALWSQDNIGAVGPITNACSYYQQIDVDYKSIE
                     DMFIFAHKINKSNINLWKYRLKLVGFCMLIKKTVLDNVGVLDELFTPGNFEDDDLSFR
                     MIESGYKLLLCQDTFIHHFGSVSFKENRNKYTDIMYINRRKFETKWGFNPEYSGMIRT
                     DLISKIDDSLEANINVLEIGCGIGATLLEIKNIYKNASIYGIEISESAGKLAKYSADV
                     LIANIEKVKLDYKKDFFDYIILGDVLEHLIDPWKIVNELKQYLKKDGYLVSSIPNIMH
                     VSVLRNLVLGNFTYEDSGILDKTHLRFFTLNEITKMFNDSNYIINSITATSMPLSEID
                     NSFIDTICKESCKEMRQQYESYQYIVKASKILDTKRYSSISMINLKYALMRIDNELDV
                     DSNLSYIFDNYNITEPCFVDDIEYLIDTFVINKATLLNRLGVEAFNRGLGDFSINMFM
                     TVLEMDRDDIDSIYNITSVLCELEEFDTAYNLIKNSSEKVQSESDIQEILCFIEGKKY
                     E"
     gene            28808..30922
                     /locus_tag="MJE33_000821"
     CDS             28808..30922
                     /locus_tag="MJE33_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBY9646789.1"
                     /translation="MNNDKICFITCVNDERLYEECLVYINSLDIPKNFSIETVALRGA
                     DSIASAYNKAMRETDSKYKVYLHQDTFIINKNFIHDILEIFKKNTDVGMIGVAGVKDM
                     PVNGIWWDSDKKAGKVYDSHTGKMDIYEFSNFEECFNEVMAIDGLIMITQYDVFWRED
                     KFDGWHFYDLSQSMEFRQNKYKVIVPKQNKPWCMHDSGVVNINNGYDEYRIKFVKEYI
                     NLDLYRENFYEFDKKKASTILKSNKHVMDCMKEELTEQLRLKNYKDVLGIVSKIAHFS
                     VFNHTGLYSCEECERALLECANNLPEIENTKLFTKKDLKRRKVLHVLSEGYSTGGHTR
                     LVKNWIEIDKDSVHSVVTTWQSDTLPDWLLKAINDSGGEVISLDLISDDYLKRAVALR
                     KIAYDWADIVILYVHMWDTIPVVAFGVDGKIPILHVNHYDSTFWLGTSIADIVIDFSE
                     TSQLITLDKRGIDKSEILPMPLKEKEVFSKSEVRKRYNLEDDVTIILTIASERKYNSV
                     NDINYFKIAKTLIENTDNTIIIVVGVDTENLYWKTLSNVTDNRVICFGTTANIDDFYK
                     ISDIYLESFMVGSHTSKLDAILYDLIPVKFKNEVSPIFTNMWNFIDKFDYKNIKEVID
                     MIRRYNTSEFDRDKIKAKQSEIKHFVIDKHCIEIKNYIDKIYNEVNHHKVKYDLYKND
                     EIEDYQLFVALYGYMVKNKNMS"
     gene            30931..31926
                     /locus_tag="MJE33_000822"
     CDS             30931..31926
                     /locus_tag="MJE33_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HBY9646790.1"
                     /translation="MNNIHINKFEKSCEKNWDYFVENKSINGTFLQTRNFLNYHEEGK
                     FTDHSLIICKGEEILAVVPACETYEDGERIFFSHLGSTFGGIIIRSGFNDIRHVNAIT
                     NKLEIYLQEQNFDKVYLKNTSRIFCKEDTSLLEYFLFKNNYMFYDELSMFLELSKYKE
                     DILANFKYSKRRDFKYSLKNNLTFKRLYSDEDIVCFFNLLSNCLDSKGAKVIHSVEEL
                     LEFKNNRLKDIVEFYAVYIDDIMICGSMVFNFQERVYHTQYLASNSTYNKYYPMIFLY
                     YNLIKDALERNFKYLSFGISTLNRGKYLDEGLATFKEGFGSSAICNRTYYKNINK"
     gene            32018..33250
                     /locus_tag="MJE33_000823"
     CDS             32018..33250
                     /locus_tag="MJE33_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HBY9646791.1"
                     /translation="MKRLMILGSIKYFENIVMSAKREGIYTIVCDNRIDTPAKKICDE
                     AIDVDVFDFDKIKNIAIDKKIDGILTGFTDSLMKPYVYIANELNLPCVISCNQLESVT
                     NKAVMKNYFKNNGIPTTDYCIIESLKDLEKIQGLNFPLVIKPVDGYGSRGVYFVSNIE
                     ELIEKFEYSKEASQDGQVIIEEFYESEEIQGLAWVHNGESHVFYIGDRELVNIHTGRA
                     GKPDRLIYPSKYCFQYEEEIKSIYQNITQAFGIKNGPLYVQMLVGAYGIKVSEVMPRL
                     PGGCDYMAISQITDLDIGSLFANFSVNNEVNFDEIKKYNMHLSKCVYALPIYIRPGTI
                     KKINNIDKIENLDYVTQFLLLVKEGDTIGLYEDERQDCGRVYGVANNVFDANIKKKCI
                     HEMIEILDENNENMIENF"
     gene            33270..34214
                     /locus_tag="MJE33_000824"
     CDS             33270..34214
                     /locus_tag="MJE33_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HBY9646792.1"
                     /translation="MKLITHEQIMSLNISQKQGYEWIKESFLTKKDCQLPPKISMVEE
                     GHVFYNVMPSLNHNHKIGGIKMVTRLPGRNPSLNSEIFLYNMEDAKLKAILDGNYITA
                     FRTAAVAALAIDTFAKKDFNTIAIMGLGVTASAIMNMLSYTLKDKNINIKLLKYKDQA
                     ESFVKKYEKNEQFSFEICDTHEELIVDSDVVISTVTYTDEYLSEFKWFKKGALLLPVH
                     VMGFGNVDYLFDKVYLDDYNHLKHLENIDRYKYSCEFYDVLSKQAKGRENDDERIISY
                     NIGLSIHDILFANKIYELCETLSCEEIELKEPKEKYWI"
     gene            34308..34715
                     /locus_tag="MJE33_000825"
     CDS             34308..34715
                     /locus_tag="MJE33_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
                     /protein_id="HBY9646793.1"
                     /translation="MDKYNLIRFVSIGNEYIGNLVALESNKAIPFDIKRVYYIYDVPN
                     NVKRGFHAHKNLEQVLVCLNGSVKIKCYDGVKETIFNLHKNDVGLYIGTGVWHEMFDF
                     DEKTVLISIASEHYNEEDYIRDYKEFLKYLNNV"
     gene            34802..35893
                     /locus_tag="MJE33_000826"
     CDS             34802..35893
                     /locus_tag="MJE33_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HBY9646794.1"
                     /translation="MLVKFNNLLKQFQTHKEEYEQKIKDVMESGYYILGNEVKHFEEE
                     FANYVGSKYCVGLASGLDALIIAFRALGISKGDEVIVQSNTYIASVMGISINGATPVF
                     VEPNEYYNIDSSKIEEKINSKTKAILVVHLYGQASNMKDIQELCDKYNLYLIEDCAQS
                     HGAKYDGKMTGTFGDIGCFSFYPSKNLGAFGDAGAIVTSNEEIADKVKILRNYGSEKR
                     YYNKFVGYNSRLDEIQAGLLRVKLKYLNEIEIEKSKIVDRYLNEINNPKVILPKIENN
                     CTHVWHLFVLQVEKRDTFMEYLKKSGIESIIHYPIPPHLSEAYAYLGYKKGSFNIAEN
                     YSDKVVSIPLFIGMEDEEVSYIIKIINEY"
     regulatory      36202..36292
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            36701..37018
                     /gene="flgB"
                     /locus_tag="MJE33_000827"
     CDS             36701..37018
                     /gene="flgB"
                     /locus_tag="MJE33_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HBY9646795.1"
                     /translation="MNTYDIIKMGLDATNLRSQTIANNIANINTEGYKRHYITFEETL
                     NDAMPKIEEKTDNSKGLRVDGNNVDIEEEKVNQAMATLQYNALISFANGKIAMTKSII
                     SGR"
     gene            37026..37430
                     /gene="flgC"
                     /locus_tag="MJE33_000828"
     CDS             37026..37430
                     /gene="flgC"
                     /locus_tag="MJE33_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HBY9646796.1"
                     /translation="MSVFSGMRISASGLTAESLKMDIISSNVANINATRGPNGEAYRR
                     KVVSFEENYDNKLGMLGVKVTSVEDDKSPLKEVYDPSHPDAKPNGYVEYPNVNILVEM
                     TDLITASRAYDSNVDTLNAQKSMISKALEIGK"
     gene            37440..37757
                     /gene="fliE"
                     /locus_tag="MJE33_000829"
     CDS             37440..37757
                     /gene="fliE"
                     /locus_tag="MJE33_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HBY9646797.1"
                     /translation="MPITQINNLMQGAKLDNLIGEAKPLTLNNKEEKKNFEDVIIDTI
                     DKVNFQQVTADDMKEKFIKGEDVSMHEVMLIGQEAQLSMQYLIEVRNKIHDAYQEMNK
                     VQI"
     gene            37782..39332
                     /gene="fliF"
                     /locus_tag="MJE33_000830"
     CDS             37782..39332
                     /gene="fliF"
                     /locus_tag="MJE33_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HBY9646798.1"
                     /translation="MITSFKNKIKEQVAKSRTKISSLKKGTKIALGIGVIAICLALVF
                     TAISASKNKYAVLFSELDAQDAADISAELESKKIDTKVKGSTIYVPRTEVDKLRLELA
                     PSLTGGSKGFALMDDSSSIGLTDEEFQIKKQRMIQGEIEKTIKSFPQVEDTRVHITFG
                     ESSVFEKEAVPGSAAVYLMLKPGTTLEKNQIKSVMALISGSVTNIPEKNVQVIDNKMN
                     LLSEGILEDENVDENNQPINSGISVDKNKASEQALNKQLEKSILEMLEPIFGKGKVKA
                     TVNADLSFDTAEKTEIKIDPDKVAIKETRSKNSSKSAANNVQGVDANMNNQGNNNGTN
                     TEDSLDESFEYETGKIETHTIVAPGELNRLTASVAIDGKIAKGVQADVEDIVNNAIGM
                     DGARGDTLSVVSMVFDPEGKKEAKQELEEIKKEGFKKNIIKAVIGVVAVIAVGAIGYL
                     IYKKRSEREDMDYEDEDYISKSDSVDRKDNPLLGDDPELTLEEAVKMYAEEKPDQVTE
                     IIKTWLNE"
     gene            39345..40415
                     /gene="fliG"
                     /locus_tag="MJE33_000831"
     CDS             39345..40415
                     /gene="fliG"
                     /locus_tag="MJE33_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HBY9646799.1"
                     /translation="MSTEITNVNAFDLSDINESDKNEKVEGEGLKKAAVLLMTLGPNV
                     SSDIIKSLPDKQVQKIGLEIANISSIASKERREILKEFVEINRAKDYVIEGGLEYARE
                     LLSNALGNVKANKLLEGISINTSTKPFKHIIKLDVKKILEILRNESAQTIAVLLAHME
                     PENGAEVLSYLDFDLQNEVAMRISSMGSISPEVIKVIESSLEDKLHSISGDDFNDYGG
                     VSTLVQILINSDRKTEKNILGTIEEQNPDLAAQIKSHIFVFEDIVKLDDISIQKVLKE
                     VTMKDLVYALKGVDDEISDAIYRNQSSRAADALKEEMEMVGVIKVTQVEASQQKIANI
                     VRKLEREGAITIVRNFGGDFVE"
     gene            40408..41139
                     /locus_tag="MJE33_000832"
     CDS             40408..41139
                     /locus_tag="MJE33_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HBY9646800.1"
                     /translation="MNNKIIKSKKALYKGVLDLTIIDKVRVGESNVNKENDCRFRDEV
                     IKEAIESAEIKKNEILEEAKINAQNIEKQAYEKGYSQGQKNGYEDGYKESYDEYTQKA
                     KDEADFIKNQANLILLQANEKMTEYIKEKNKEIVRLAINMASSVLKKHFEDEESMSDL
                     LKQIIQDYEGSSSFIVRCNSFYKDNIEKEFELFKEETSLKSSVFVIADDSIDKGNAII
                     QKENGRIIVGIDCAMEKLKEELLGE"
     gene            41143..42462
                     /gene="fliI"
                     /locus_tag="MJE33_000833"
     CDS             41143..42462
                     /gene="fliI"
                     /locus_tag="MJE33_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HBY9646801.1"
                     /translation="MYKTDISIDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKA
                     FVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGELTLIAPGCKVISKGIPLSVMCSD
                     NLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
                     EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKK
                     SVVVCATSDKAPLVRLKGALTATAIAEYFRDQGKKVILMMDSVTRFAMAQREVGVAIG
                     EPPAQKGYTPSVFAMLPKLMERTGTSDKGSITAFYTVLVDGDDFNEPIADTTRGILDG
                     HIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINI
                     GAYASGTNKKIDEAIYYHEHIINFLKQGINEKSSFNETISSLRRVFE"
     gene            42493..42939
                     /gene="fliJ"
                     /locus_tag="MJE33_000834"
     CDS             42493..42939
                     /gene="fliJ"
                     /locus_tag="MJE33_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HBY9646802.1"
                     /translation="MELDKNFKFRLQKVLDLKVKDEEEIKMEFAKIQQKKIDIETNLE
                     NLESNYSKYSISKNNDSIQNQKITINYLLALNNSIMDLSEELDKSTNELEKARKQLIS
                     KQIERKSLEKLKEKKYGQYYKEENLKEQNTNDEFASMSYLRNRQVL"
     gene            42968..44173
                     /locus_tag="MJE33_000835"
     CDS             42968..44173
                     /locus_tag="MJE33_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HBY9646803.1"
                     /translation="MKIQADSYNIIKDIGDRKSSPKNKNIKNNKFEDNLYKATDHKKV
                     KDRREVSKQNISSDKPENSDNECHLDEDKSNHLEDENTYEKDCSKNNEALQQLLNLLK
                     DNYKKDKDINTILNNIEKLSISDELKQELKLNKNILNKIANQNSELNKANINNSNDIE
                     NLDIVKLRELLEDNANKDIEVITKTTENDTKNIDLYSFDSNRMDNLNSNKQKDDSSDI
                     LEKLAGVNGNKSGNFGQVINKNVDMNKIKNDNVNLKQIESNVMDDSIKAIKHMKTNDI
                     QELTIKLRPKELGDMNIQLLKDGESMRAVVTVFNKDVFDSINKNIADLKQHLELTNVN
                     IKDVSVQMHSDNTNTSDTFDKAFEQQNRQNNQENMSKKNTNDKHNNVVIEDDIKENTI
                     DDDRVDLLA"
     gene            44191..44799
                     /locus_tag="MJE33_000836"
     CDS             44191..44799
                     /locus_tag="MJE33_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgD"
                     /protein_id="HBY9646804.1"
                     /translation="MYGIATALAGTKGNYRSNNLSQLERNINDIANKQVRSKGNQTVT
                     DNGTPIIGQGDDADKDLFMKILVAQMGNQDPMNPQDPTEYVSQLAQFATLEKMSAMNE
                     KLDVLLALSNSTLINSALSMATSLIGKEVEVSGEEKEDENIVGVVKSTYIKDGEVHLE
                     LEVDGSDKTVGVKYEKLLKIGEVDENSGEENDSSEHESPEEA"
     gene            44826..45809
                     /locus_tag="MJE33_000837"
     CDS             44826..45809
                     /locus_tag="MJE33_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HBY9646805.1"
                     /translation="MIKAMYSGVSGMKANQTKLDVIGNNVANAGTTSFKKSSARITDS
                     FYQTVLYASAPTAALGGTNLGQVGVGSKISSINKDMVQGNVQPTNRPSDLMIDGDGYL
                     PVVRQGTVMYTRDGSFNLDTGFKAADGALKNIKGGRLVNGDGYMLQGMIYDGEYKADT
                     GEFVKKGDAKGKEPIVIPLQSISPKDGTVQNVLEYNISKDGTVEFLLSDGQRTQFVYD
                     IPDGAKVGDTPAGIDKLKGTIQKVQTYAFQNPAGLDAEGGNLFKPSANSGDPQIAGAT
                     GTIIQGAIEASNVDIAEEFTEMIIASRSFQANSKIISTSDEILQEIINLKR"
     gene            45822..46025
                     /locus_tag="MJE33_000838"
     CDS             45822..46025
                     /locus_tag="MJE33_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HBY9646806.1"
                     /translation="MIRVTDLNGRKYILNSDLIVRIDSVPESRILLTTGEKNLVKESV
                     DEIVDKVIKYKRKINVGYIRSEK"
     gene            46025..46843
                     /locus_tag="MJE33_000839"
     CDS             46025..46843
                     /locus_tag="MJE33_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HBY9646807.1"
                     /translation="MKKTDSLTFIGLIVSTVLVLVGAAKGSSSGLKNFFDVSSILITV
                     LGSFGALMITFTIDDIKLIKNALQYSFKTMSVSKLDLLEQFKTLSKKARKEGLLSIES
                     DVMGIEDPFMKKGLELCIDGLEINEIRSILELEIDSVEDTNNRYSKLFKTWGTFAPAF
                     GMLGTLIGLIQMLADLTSPDLIASGMGKALITTFYGSLLANIALNPIAYNIDEKTEKE
                     IYVKEMMLEGIISIQSGESSIVVEERLATYLSNNEKLEIMKSNKNTERAMSNGA"
     gene            46833..47528
                     /locus_tag="MJE33_000840"
     CDS             46833..47528
                     /locus_tag="MJE33_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBY9646808.1"
                     /translation="MARKKNKKKDEINPDAWLATYADTITLILTFFVLLYATSVVDME
                     KFKNIADALKGQFSGTSIFSTGSSTSEKSPIDSVLVDKNDKSNLEQDLKDKVNNSNLQ
                     NSVTVKDDSRGILLELDDSILFDSGISELKVDSKATLNKVYDMIKMMKNKIVIEGHTD
                     NVPVKSSTHESNWDLSSSRAVSVVRYFVEEKGMDPRLFSATGCGEYQPLVSNETAKGR
                     AENRRVNILITID"
     gene            47540..47983
                     /locus_tag="MJE33_000841"
     CDS             47540..47983
                     /locus_tag="MJE33_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HBY9646809.1"
                     /translation="MFKNKKIIIIILILIIFSAGAGFFILGEGGNKQVDKIAGLFIPT
                     GKGKDEVKTENLDLEEMVLKLADEGVRYLKVSITVSYDSSYKDIEKNTPLIRDNIINC
                     FMAKRADDFTADNLKNIKEEIKNTLNIQLGENVIQNIYFTNIVVQ"
     gene            47998..48372
                     /locus_tag="MJE33_000842"
     CDS             47998..48372
                     /locus_tag="MJE33_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliZ"
                     /protein_id="HBY9646810.1"
                     /translation="MILYLLKILICIPIILILIVLSLKISQGTLLKKYNDKYVKILDI
                     VNISKNNSIIILKIGEEGCIMASSSSSMSKIKDLTKEEILKIENDIKERNFKIQDFKM
                     ERIKKKYDFSRYIGKFTKKEDK"
     gene            48373..49038
                     /gene="fliP"
                     /locus_tag="MJE33_000843"
     CDS             48373..49038
                     /gene="fliP"
                     /locus_tag="MJE33_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425215.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HBY9646811.1"
                     /translation="MELLSKLPLADVPYTSSMQLFLFLTALMFLPFVMFMMTSFVRIV
                     ISLSFLKSALGAQQAIPSQILVGLAIVLTIFIMRPVLNEINEKALQPYMKEEVTMEEA
                     MKEAEGPIKEFLLTQTRQTDLDLFVEQAGLKEKKLTRENIPLSVVVPAFAISELKTAF
                     QIGFLIYIPFLIIDLVVASVLMSMGMFMLPPVMISLPFKLLLFVMVDGWNLIVKTLIL
                     GFG"
     gene            49051..49320
                     /gene="fliQ"
                     /locus_tag="MJE33_000844"
     CDS             49051..49320
                     /gene="fliQ"
                     /locus_tag="MJE33_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HBY9646812.1"
                     /translation="MSEFMIMSVLKNGMFTGAMIAAPVLIVSLVVGLLISVFQATTQI
                     QEQTLTFVPKLIAILLVVAIGGSWMLHTLISFTSEIFNMISLITK"
     gene            49332..51146
                     /locus_tag="MJE33_000845"
     CDS             49332..51146
                     /locus_tag="MJE33_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused FliR family export protein/FlhB family
                     type III secretion system protein"
                     /protein_id="HBY9646813.1"
                     /translation="MIFVFIRIVAFFGTLRILFPAGTPALFKSLFAIIISILISSTMQ
                     IEYATNIDNIVLFTLYGINETITGIMLGYITNLCFYSIRMAGSMMDQQMGLSMINMFD
                     PNSMTQTTLIDNLMNWTALMIFFSMDGHHVLIRGIRYSFELIPIGKPFVDNNIDYIIN
                     IFVQCFLTGFKIAIPIVLCLLMADFILGLISRSVPQLNVMIVGMPLKILVGIALFIIS
                     IPLIVNQISHLLSQIPKMYEGTFALAPMFFMGSTDKTEEATPKKKGEQRKKGNIAKSR
                     ELPVAMTLLAFTLLVPTLFSYVVDTLKSSLNYFLSLDFYMNINYSNLEKLVIAGLMDF
                     FKIFLPIAIPFLVLGIIANLFQVGILFTGETLKPNLSKLNPVSGFKNMFSMRSLSTLI
                     KDIAIISILAYIGYTFFQDNYLDILKLGNIYLPTLMYTVKDLVYSILSKICVAMIAIA
                     VADYVYQRYSHKKQLRMTKQEVKDEYKNSEGDPEVKAKIKQKQRQISSQRTMQAVPSA
                     TVIVTNPTHLSIAVRYEKGKDQAPVVVAKGADYLAFKIREIAKGNDIPIIENKPIARL
                     LYKQVEIDQEIPEDMYQAFAEILVAVYKIKNRYKVPKR"
     gene            51155..53230
                     /gene="flhA"
                     /locus_tag="MJE33_000846"
     CDS             51155..53230
                     /gene="flhA"
                     /locus_tag="MJE33_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HBY9646814.1"
                     /translation="MNKFNLKENMDVIVAFGVVGIVLMIIVPLPTFILDILLAINISL
                     SVLILLMTLFTTNVLDLSIFPTVLLVTTLFRLGLNLSSTRLILTQGYAGEVIEAFGSF
                     VVGGNYVVGIIIFLIILIVQFVVITNGSGRVSEVTARFTLDAMPGKQMSIDADLNAGV
                     IDDKTARKRRKELQQEADFYGSMDGANKFVKGDAIAGLIVTAINIIGGIVIGAVMLNM
                     TLMGAAQTYVRLTVGDGLVSQIPALLISTSAGIIVTRASTDENFGGLVGKQLTAIPKA
                     VMMTGCVLIVLGMMPGLPKPAFFSLGIGAIAAGYFLGREKKDTLEDLEFEKPDMVNPD
                     KNEVEDVTNLINVESIEVEIGYGLISLADESAGGDLLQRIASIRRQCAIDMGIVVQPI
                     RIRDNLQLNPNQYNIKIKGNVVGGYEIMPNMVLCMDPMNQGFSIFGIKTIEPTFGIEA
                     LWIESDKVEDAELKGYTVVDSSTILITHLLDIIKSNAHELLGRQEVKTIIDAARENYS
                     AVVDELIPDLMTLGEIQKVLKNLLREKVNIKDRVTILETLADQSRNTKDIELLTEYVR
                     IALARSICNNLVDDDKTIVVATLSPETEELISNNLQRSVNGTYPAVDPENTNKIFESI
                     QEVMSNVYFNHNIPVIVVSPKIRAPFRKLVEIVYPNLTVLSLNEIPNDIKIKAEGVVS
                     IYDDEKVYS"
     gene            53205..54221
                     /gene="flhF"
                     /locus_tag="MJE33_000847"
     CDS             53205..54221
                     /gene="flhF"
                     /locus_tag="MJE33_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HBY9646815.1"
                     /translation="MMTKKYTANTIQDAMNLAKMELGDNITLIDKKEVRKSGIQGIFS
                     KKDIELTIGWEKRENIEQKDLKREIEQLKLIINNMGFDNKNDNDIDKICKNLLSLELN
                     EEIVESIRIDLQEMKFNGIDTSKNLVEILKKKIKIENQVINGKIALVGPPGVGKTTTI
                     AKLAAKLVFEENKKVGVITIDTYRIGAVEQLKIYTDIMNIPFKGVISPDEMELALDEM
                     KDCDVVLIDTTGRGYKNSMQILEIKNLIDKAETDNIHLVLNCTTRESDTKAIIDSYRN
                     VNFKSLIITKLDETITYGSIFNIMNYAQKPISYITTGQNVPDDIIKPNEDKIIRLLLG
                     VESI"
     gene            54218..55096
                     /locus_tag="MJE33_000848"
     CDS             54218..55096
                     /locus_tag="MJE33_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HBY9646816.1"
                     /translation="MIDQAQILRKMAMEKRGLDEFIVENENVPKIITIASGKGGVGKS
                     NLATNLSICLTKLNKKVLILDADIGMSNIDIIMGVNVKGTIIDVINGEKNIEDIISQT
                     KYGVNIISGGSALNHIEDFTEAQRNKFIHSIEQIHDVDFIIIDTGAGMSKSLLSFIYC
                     STEFFLITTPEPTSLTDAYSLLKAISNFGIKRSANIIINRVIDLEEAKSTYKRIKMVV
                     DKFLNLNLELYGYIIDDKKVSLCVKKQIPFILEYPTSIASKCIIAIAKRMVSQSREDI
                     VDNKSFLRKMFSIFKS"
     gene            55110..55811
                     /locus_tag="MJE33_000849"
     CDS             55110..55811
                     /locus_tag="MJE33_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HBY9646817.1"
                     /translation="MNREELIKENMPLVKSIARKFFIPGKGFEYQDLVNSGVIGLIDA
                     INKFDAEKGAKFSSYSYIKIKSAILDEIRNQSPISKHNLTKVNKYNRVVEKLQSKLLR
                     EPTSYEIAKELKVSEKELHDIESNIDMLNIVSLNYVIFEDTNETVQDVISDREEEAPE
                     NIIEEEEKLEILSKAISNLNEREKLILSLYYYEDLNLKEIGKVLGVSESRVSQLHRKS
                     IRNLRNKIKELKYSI"
     gene            55827..56249
                     /locus_tag="MJE33_000850"
     CDS             55827..56249
                     /locus_tag="MJE33_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HBY9646818.1"
                     /translation="MIQIILIVVSILIIVTIVINLHKESKNKDNRYFDKIFEQEYEAN
                     DRLIIAEKRRFFLDKVLAEKRINSHIKVYENYNLIKANEERKENIFIESKKEVRQENE
                     LSLKVKHLISSGYDDVEICKMLDIGKGELLLIRSCYKI"
     gene            56234..56419
                     /locus_tag="MJE33_000851"
     CDS             56234..56419
                     /locus_tag="MJE33_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HBY9646819.1"
                     /translation="MLQNIRILLCDKKVFLGIGIGILISTLCIMLFSNNDMSKAEIEK
                     KARALGMKYTSEMKVLE"
     gene            56460..57233
                     /gene="flgG"
                     /locus_tag="MJE33_000852"
     CDS             56460..57233
                     /gene="flgG"
                     /locus_tag="MJE33_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HBY9646820.1"
                     /translation="MIRGLYTAVSAMITNQQKQNVVVNNLANMDTNGYKSKQLLTKSF
                     DELKLEGYNNYANGTKQKQIIGDISPGISMDETITNFNQGPIKTTDNKMDVAIQGKGF
                     FLVSDAAGNQFYTRNGNFRTDNQGDLITSEGYYVLGTNTATGAVGHINVGNQKFEVAK
                     DNTISLDGNAMYKFNIVDFNNYNNLKKEGDNLYSGGGAMKVNNALTIQSAIESSNVDM
                     VSEINNMMTISREYEANQKIIQAMDSKLAKIASEIGSVR"
     gene            57254..58015
                     /gene="flgG"
                     /locus_tag="MJE33_000853"
     CDS             57254..58015
                     /gene="flgG"
                     /locus_tag="MJE33_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HBY9646821.1"
                     /translation="MYTMFYTSKTALSANQSKLDIISNNIANSDSTGYKKINMEYSDL
                     VDEVLNRPSYPTNGNDISTGTGAKATAPMRNYEQGALAPTDSKSNLAIDGEGFFRVIR
                     KDGTYAYTRNGGFNVDALGKIVDDNGNILDVQFDAGYNYNNTNIDSTNLTVSRNGELF
                     VDNKKIGKINLYQPIGTQNFISEGDSLFVATDAAQIKQVEKVDIVQGYRERSNVSLQE
                     EFVDLIATQRAFQMNSKGIKTADEMWQIANNLRAK"
     gene            58038..58919
                     /locus_tag="MJE33_000854"
     CDS             58038..58919
                     /locus_tag="MJE33_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBY9646822.1"
                     /translation="MNDMKEYDFGKPQSYSRENLTCLNFLLAEFCKKYKNYIIYELKC
                     NSSMVVDSIDQVNYQTFLDRVTSSCVVVQNAMEPVMKNFSFRIDRSVADMWIDITSGG
                     TGVVKDNDRELTELDKKVLLHLMDDLVRNMHLFEGFENIQVLDTHTHINLPQLCSPTA
                     PVCVVDLKVLNDNEYIGSVSLCFPYNGLEALLESISVIEFFDNDVGDDSEEFTQKIYN
                     SVSNIELSVIAEIGKVSINVEDLLKLEVGDVIVTNKKINDYIDIFVENSKSYTATPGF
                     ISSKKGVKINDAVGKEV"
     gene            58922..59296
                     /locus_tag="MJE33_000855"
     CDS             58922..59296
                     /locus_tag="MJE33_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBY9646823.1"
                     /translation="MDNSNLISDEEAKMLLNDKVESEVDGDDELALDVSEVNRNNIQR
                     ILDLKLELSVVIGRTKMTLRDILNLHKGSLIELDTLADQDVEILIDNKVLAYGKVVVV
                     DLNFGVKITSIVSEEDMVKSLV"
     gene            59328..60731
                     /locus_tag="MJE33_000856"
     CDS             59328..60731
                     /locus_tag="MJE33_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBY9646824.1"
                     /translation="MVLILNLNDIKKDILKLNKKDSCSLYNESINNINNNCISTGLKF
                     LKQARDLNPDDVDILNLTGLVNLLKCNFDDAVESFYKSSLCEKTALCEKYINMLTSEE
                     FLIFFEKYNQLIRLINKGMYKEAIQGFKLITDQYCDLIEPYELLALLYDKEEEYIKFD
                     ECLEVLKIIDKENYLLNQENNTLYMYNSANIQTKSNIKVDDYKNEFLHKSSSNHKIND
                     YNDVTKEKSSSKNQKSSDSNKKKSIKKNKYLYIAIILGILLVGQSIYSSYKMNNLTDK
                     ISKNNETKNEESIDSNLTKKENNIEENEDMSKASNINKSNKNINKEDDVKSKKNTVEN
                     EDDKDSDLFTEDELMSKANTLKSEKKTKSSIQLYKKVADIGENKGNTSEATYQVAILS
                     EKLKDYKTAEEYYKMYVENYSEKDAYFDESYYNLGMMYYNNGDLKNSKLTLKKLVNKV
                     PNSMYNNSKVKEILKEE"
     gene            60847..62784
                     /gene="htpG"
                     /locus_tag="MJE33_000857"
     CDS             60847..62784
                     /gene="htpG"
                     /locus_tag="MJE33_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HBY9646825.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISESDARNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEIVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFNVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITLQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEAVGERVKNYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLLIEL"
     gene            63172..64302
                     /locus_tag="MJE33_000858"
     CDS             63172..64302
                     /locus_tag="MJE33_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBY9646826.1"
                     /translation="MNFNYNLPVNILFGRGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKSTGCDCVLGLGGGSIMDA
                     AKSIAFSYKNPGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKASIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPLADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLRDVMH
                     GKGLAALTPTIIESSWDSDIERFGNISKVLGGTCAEDCSDAIRNFLKKIDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEQIKEMYHKSL"
BASE COUNT        26422 a         6608 c        11825 g        19448 t
ORIGIN      
        1 ggtagtagtg gcgaagacac cgtccgaagt agcttggctt tgtaaccacc tctgttgtac
       61 tgtttaagta taaataagtg tgttagccac caagaatccc cttgctttag ctatggggag
      121 tgtgtcaata aaatacttta atgaaaaata gctaacttta attgtaggat attcttaata
      181 tatttgaaat aaataaaaat attgaaggat ataatattca attggtatat aaataataaa
      241 tgatagttaa attttacctt agaggctaat taggtgttta atcaaagtta atgcctttag
      301 aatacaaatg tattctaaag gcatttttac tatgtatttt catttatatt actaaacatt
      361 atctattttt atatacattt ctatgtattt tattttcatc aaatcggtct ataaactcat
      421 ttttttcttc actatatcca atagatataa gtgcataagg aactaatttt actggtaagt
      481 ctaaagtttg tctaacttta tttaccactt tatttttagg aaatactcct gcccaacaag
      541 cagctagacc ttgattagta acttctaata ataaattttg tgtgcaagca cctaaatctt
      601 gtatccattt accaggtttt aagaatttat ctctatttcc aagcactact atacataatg
      661 gtgcaaattc tatatgcttt gctctatgct gtgactttga gagctgtttc agcagttcct
      721 tatcatctac tataataaat tcccaaggtt gagcattttt tgatgaaggt gcttgcatag
      781 ctgattttag tatttcagtt attttatctt cttctatatt tttatctata tatgttctta
      841 ttgaatgtct attttttata acttctaaca tacagattct cctttaaata aaattatttc
      901 tatttatata atatcattac tttaaatttt agctattact tatataaaga ataaactttg
      961 tgttggtttt atctaagtaa aagtaacatt cagaactaaa aaacaagact ataaatccaa
     1021 agtattaatt aaaaaagaag taataaaaaa taatatacaa gatttaaaaa agagtattgg
     1081 gcttaaaatt taattagaaa accaattttg agataatctg ttttttattt taacaaaaaa
     1141 acataaataa agttcgatac atagcgaatg aaaaaattac aagaaatatt ttttttaaaa
     1201 aatttcaaaa aagagttgat aatatatgta tattaatata aaatttaagg tgtataattc
     1261 gtataataat acgaatgatt tgtagtgtaa aataacttat aaacggagag gggaagtcta
     1321 atgaaagtag cagtagttat gggttcaaaa tcagattatc caaaattaga agaagggata
     1381 aaactgcttg aaaaatatgg aatagaagtt gtagcaagag cattatcagc tcatagaaca
     1441 ccagaacaac tttcaatatt cctaaaagaa atagaagatg atactgatgt aataatagca
     1501 gcagcaggaa aagcagcaca cttaccaggt gtaatagctt cacaaacatt aattcctgta
     1561 ataggattac ctattaaatc atcaactatg gatggactag actcactttt atcaatagtt
     1621 caaatgccaa aaggaatccc agttgcaaca gttacaatag acttaggttt aaacgcagct
     1681 ttattggcat tacaaataat gactttaaaa tacccaaaat taaaagagga tttgaaatca
     1741 tatagagaag agatggcaca aaaggtgtta gaagacgaca agaatttaag ggggtagcag
     1801 aaatgttatt atatgaagga aaagcaaaac aagtatattc tacagataat gaaaatgaat
     1861 atgttgttta ttataaagat gatgcaacag catttaatgg agaaaaaaaa gcagaaatat
     1921 cttcaaaagg tatactaaat aataagatat caacaataat ctttgaaatg ttaaaagaaa
     1981 ataatataaa tactcacttt ataaaaagtt tatcagatag agaaatgtta gttaaaaaag
     2041 tagagatttt accattagaa gtaatagtaa gaaatattgc tgctggttct atctgtaaaa
     2101 gagttggtct tgaagaaggt gtagttttcg atgagccaat ttttgaaata agctacaaaa
     2161 atgatgctta tggtgaccca atgctaaatg atgattatgc agtggcaatg aagttagcaa
     2221 ctagagaaga gttaaaattc ttaagagaag aaactttaaa aataaacgag ttactaaaag
     2281 catttttctt aaaattaaac ctaaagttag ttgactttaa aatagaattt ggtaaagatt
     2341 ctgaaggaaa tataatattg gcagatgaag tatcaccaga cacttgtaga ctttgggatg
     2401 taaatacaaa tgaaaagtta gataaggaca gatttagaaa agaccttgga gaccttgtag
     2461 aaggttatac ggaagtactt tcaagaatga ataataaata ggaggcaaat gtatgtgtgg
     2521 agttttaggg atttactcta ataaagatgt aactaaagaa ctatattatt ctttatattc
     2581 tatgcaacac agggggcaag aaagctgtgg tttagctctt ttagatgatg gagaaattaa
     2641 gtacaaaaaa gatatgggat tagttggaga tgtttttaag gaaaatgaat tatctaagtt
     2701 aaaaggaaat atagggattg gacatgttag atattcaaca gctggaggaa gtcatgtttc
     2761 taactgtcaa cctttagttg gaagttgtag aaaaagacaa ttagccatag ctcataatgg
     2821 aaatctagtt aatgcaaatt atttaaaaga tatgctagaa gaagatggat atatgttcca
     2881 aactaattca gatacagaag tgattttata tatacttgct agatattaca aaggggatat
     2941 agtagaaagt ttaaaagtga caatggatta tataaaaggt gcatatgctc ttgtaataat
     3001 gagtcaagaa gaattggttg cagttagaga cccacatgga ttcagacctc ttgtacttgg
     3061 taaaaagggt gatgaatata tatttgcatc agaaaattgt gcaatagata tacttggcgg
     3121 agaagttata agagatgtag agccaggaga gataatagta gtcaaagatg gagaactaaa
     3181 atcatatttt tattcagaaa attataaacc agttaagaaa agttgtatat ttgaacacat
     3241 atactttgca agaaatgatg caactataga caatgtaaat gcatatgaat ttagaattaa
     3301 atgtggtgag agattagctc agaatgaaac tgtaaaggca gatatggtag ttccagttcc
     3361 agattcaggt tggccaggag ctattggtta tgcaaatgct agtggattaa aaataagtga
     3421 aggcttagtt aaaaatagat atgtaggaag aacatttata aaacctacac aagaagaaag
     3481 agaaatagca gtaaaaataa aattaaatcc tctttcaaca ataataaaag gaaaatctat
     3541 aattttagta gacgactcaa ttgtaagagg aactacatct aaacaattag taaaatcttt
     3601 aagagaagca ggagcaaaag aaattcatct tagaataact tctcctccag tagcttactc
     3661 ttgttactat ggaatagata ctccaaatcg ctctaaatta attgcatcaa gtaacaacgt
     3721 agaagaaatg agagaatata ttggatgtga tagtttaaaa ttcctagata tagaaggaat
     3781 gttagatgca acagaacata agtctacttt ctgtaaagct tgttttgatg gagaataccc
     3841 agttaagaaa attgataagg aggaactact ttcatgttaa catataaaga atcaggtgta
     3901 gatatagatg aaggaaatag agcagtagac cttataaaag gtaaaataaa aggaacttat
     3961 gatggaaatg tagttggaga tttaggtaac ttcagtggat tatacagttt aaaagatttt
     4021 gtaggtatga aagaaccagt tttgcttgca tctacagatg gtgttgggac taagttaaaa
     4081 atagctcaga tgatggacaa gcatgatact gttggaatag atttagttgc aatgtgcgtg
     4141 aatgatttaa tttgtcaagg agcaaaacca ttattcttcc ttgactatat agctcttggg
     4201 aaattagtgc ctgagcatat cgaaaaaata gttggtggaa ttgcagatgg ttgtaaaatg
     4261 tctggttgtg cactaattgg tggagaaact gctgaaatgc ctggaatgta tggagaagat
     4321 gactatgatt tagcaggatt ttctgtagga atagcagaca aagaaaagat tgtttctgga
     4381 aacaatgtta aatcaggaga tgtattggtt ggtatatctt caagtggagt acacagcaat
     4441 ggattttctt ttataagaaa gatattttta gaaacttaca attataaaat ggaacaatat
     4501 gttgaagaac taggaatgac agttggagaa gcacttttaa caccaactaa aatatatgtt
     4561 aaattagctt tagatgtatt agctaaacat gacataaaag ctatagctca cataactggt
     4621 ggagggctta tagaaaatat aacaagagtt atacctaaag ggttaggact agatatcaat
     4681 aaaaaatctt gggagaagcc tcctatattt aaaatgatag aaggttttaa tgctgttgat
     4741 gagagagaac ttcataagag ttttaacatg ggtattgggc tagttttaat agtagataaa
     4801 gaaaatgctg atgatgttgt aaactttata aataatagag aaaatgacaa tgctgcttat
     4861 gtagataaaa aatatagtga attattagaa gataaagctt atataattgg tgaagtagta
     4921 gatagtcatg aaggtgtaga attatgctaa atataggagt tttaatatct ggtggaggaa
     4981 caaatttaca agcagttata gatggtactg agtctggaga aataaaaggt caggttaaag
     5041 tagttatctc aagtaagcaa ggtgcttatg ggctagagag agctaaaaat cataacataa
     5101 aagcaatttg tgaaactgat gaagacaaga taatagaaat tcttaaggaa aataaaatag
     5161 atttagtagt tttggctggt tatttaaaaa taatcagccc aaagctggtt aacgagttta
     5221 gaaacaaaat gataaacata catccatcat taattccttc attttgtgga gctggattct
     5281 atggagaaaa agttcatcaa ggtgttatag attatggagc taaagtaact ggtgctacag
     5341 ttcattttgt agatgaaggt gctgatactg gaccaatcat aatgcaagat gtagttaagg
     5401 taaatcaaga tgatgatgca aagacacttg caaaaagagt gttagaagtt gaacatagga
     5461 ttttaaaaga aagtataagt cttttttgtg aaaataaatt aaaacttcaa ggtaggagag
     5521 tgtttataaa tgagtaagag agctttaata agtgtaactg acaagactgg tgtagtggaa
     5581 tttgctaaag agttaaataa attagattat gaaattatat ctacaggaaa tacatttaaa
     5641 acattaaaag aaaatggtgt taatgtaatg caagttgaag atgttacaaa ttttccagaa
     5701 atattagatg gaagagtaaa aacattaaat ccatatatac atggtggtat actttataaa
     5761 agagataaag aatctcatgt agaaactgta aatgaacaca aaatacattc aatagattta
     5821 gtagctgtaa atctatatga ttttgaagga actttaaaag ctggaaaaag tcatgatgaa
     5881 ataattgaaa atatagatat tggtggacca tctatgatac gttctgctgc caaaaactat
     5941 aaggatgtaa tagtagtagt agatataaaa gattatgatt ctataataga aaaattaaaa
     6001 acagatacaa tgacattaga agacagaaaa aaattatcat ataaagcttt ctcaactaca
     6061 ggaagatatg atgcgttaat atctagttat tttgctggag aagttggaga tacgtatcct
     6121 gacatattaa atttaacttt ccaaaaagaa caaactttaa gatatggaga aaatccacat
     6181 caaaatggtt tcttatattc acagtcaaat gcaaaaaatc caatcttaaa ttatgagcaa
     6241 ttaggcggta aagaactttc ttttaacaac ttaaatgatt tacatggatg tttagaagta
     6301 atgagagaat ttaaagatag tgaagaagta gtttcagtag ctataaaaca tgctaattca
     6361 tgtggggtag gtttaggaaa agatgctttt gaagcatata caaaatgtta tgaagcggat
     6421 aaggtttcaa tatttggagg aatagtagga ataacttcta ctatagataa agctacagca
     6481 gagaaattaa atgaaatatt cttagaaata gtagtagcat atgattttga accagaagct
     6541 ttagagatac ttaaacaaaa gaaaaatcta agaatattaa aattagctaa aatagaaaat
     6601 agtttacaac catatgaaat gaaatattta gatggaaaat tattgataca agatagaaac
     6661 aatatattag cagaaaaaag tgaaaatgta acaaaggaaa aaccaacaga tgcacaactt
     6721 aaggacatgg aatttggtat gagagtagta aaaaatatga aatcaaatgc aattgcaata
     6781 gtaaaaaatg gacaaactct agctttaggt tgtggacaaa catcaagaat ttgggcatta
     6841 aaaaatgctc ttgaaaataa caaagataaa gattttacag gagctgtttt agcgtcagac
     6901 gcatttttcc catttgatga ttgtgtaaca ttagctcatg aatatggaat atcagcagta
     6961 gttcaaccag gtggctctat aaaagataaa gattcaatag aagcttgtga caagtatgat
     7021 atggttatgg tatttacagg aataagacac ttcaaacact aaggaggata ttatgaagat
     7081 attggttgta ggcggtggcg gaagagagca tgctatatgc tggaaactaa gcaaagaaaa
     7141 aaatgtagaa aaaatttatt gtgcaccagg taatgcagga attgcaaacg tagcagaatg
     7201 tgtaaatata ggtgatacta atatagaaga acttttaaaa tttgccaaag aaaatgaaat
     7261 aggtctgaca atagtaggac cagaagtacc actagttatg ggaatagtag atgagtttga
     7321 aaaagaagga ttaagagtat ttggacctaa taaaaaatgt gctcaacttg aaggaagtaa
     7381 agctttttca aaagaattta tgataaaaca taacatacct actgcaaaat ataaagaata
     7441 tacaaactta gaagaagcta tatcagaaat agatagtttt ggatatccag tagtaataaa
     7501 agcggacgga ttagctgctg gaaaaggtgt tgtaatacct gaaaatagag aagatgctat
     7561 agcaacttta aaagaaatga tgtcagataa gaagtttgga gctgctggag ataaaatagt
     7621 aattgaagag tttttaaaag gaatagaaac ttcaattcta gcatttgtag acaatgatac
     7681 aatagttcct atggcaagtg caaaagacca taaaaaagta aacaactatg agcaaggtcc
     7741 aaacactggg ggaatgggaa ctttttcacc tagtgaaata tatacagaag aattagcaaa
     7801 taaagtaaaa gagacagtat tagaaaaaac tttagaaggc ttcaaaaaag atggacttaa
     7861 ttttaaaggt attttatttg ttggtcttat gataactgaa gatggagaaa aagtacttga
     7921 atataatgta agatttggag accctgagac acaatctgtt ttatttagat tagaaacaga
     7981 tttacatgag ataatggaag ctatactaga taataagtta aaagatatag aaataaacta
     8041 tagtgatgat gaagctgtgt gtgttatgct tacatcagga gggtatccag acagttatga
     8101 aaaagggaaa attataactg gtcttgaaaa cttggatgat gatatagtag tattccatag
     8161 tggcactaag atgtttgatg gtaatttggt taccaatgga ggaagagtaa ttggtataac
     8221 tgcaaagtca actacagtta aggatgcagc agaaaaagtt tatgaaaaca taaagaagat
     8281 aaactttgaa ggaatgcact atagaacaga cataggaaga taatttttct aaaatggagg
     8341 ataaggtatg ttgaatactg aaaataaaga ctcaatggtt agaagagtat tggttgaaaa
     8401 aagagaaggt tttgaccttg aggctaaagc tttaaaaaaa gacttagtag aaagtttaca
     8461 catagataat atagaaaatt taagaatatt aaatagatat gatgtagaag gaatatcaga
     8521 agaagtttat gaaaatgcag caaagacaat attctctgaa ccaaatttag atgtggtata
     8581 ttatgaagaa ataccaaaat taaatgatga aagagtattt gcaatagaat ttcttcctgg
     8641 tcaatacgac caaagaggtg attgggcagc tcaatgtgta caaattgtaa atcaagggat
     8701 aagacctgct attaatacag caaaagttta tatattatca ggcaaaataa ctgatgaaga
     8761 gttttctaaa ataaaagatt actgtataaa tccagtagat agtagagaag catctttaga
     8821 gaaaccagaa acgttaaaaa tggaaactga aataccaaca acagttgaag tattagatgg
     8881 atttatagat ttagatgaaa atggattaag aacatttgta agtgaaaaag gattagcaat
     8941 gactttagga gacttacaac acgttcaaaa atactttaaa gatacagaaa agagaaatcc
     9001 aactataaca gaaataaaag tattagatac ttattggtct gatcattgta gacatactac
     9061 ttttatgact gaaatagaaa atgtaaagat agaagatggt aagtttaatg atatagtaaa
     9121 agaagcgtat caaatgtatc ttaactcaag agacaatgta tatgtgaaca gacataaaga
     9181 tatatgttta atggatatag ccactgttgc agtaaaagaa ttaaagaaaa atgggaaatt
     9241 aaatgaccta gatgaaagtg aagaaataaa tgcatgtagt ataaacgtag atgttgaagt
     9301 tgatggtaag atggaaaaat atctagtaat gtttaaaaat gaaactcaca accatccaac
     9361 tgaaatagaa ccatttggtg gagcagctac ttgtttaggt ggagctataa gagacccatt
     9421 atcaggaaga agttatgttt atcaagcaat gcgtgttaca ggaagtgctg acccaagaac
     9481 gactttagaa gatactttac ctggaaaact tatgcaaaga aaaataacta cagaagctgc
     9541 tcatggatat agttcatatg gtaaccaaat aggtcttaca actgggcaag ttgcagaagt
     9601 atatgatgaa aactttgttg caaagagaat ggaaataggt gcagttatag cagcagcacc
     9661 aaaagagaat gtagtaagag aaagacctga agcaggagat gtaatcgttc ttcttggagg
     9721 aaaaacagga agagatggct gtggaggagc aactggttca tctaaagaac atagtgaaga
     9781 gtcaatctta acttgtagtg cagaagttca aaaaggagat gctcctaatg aaagaaaaat
     9841 acaaagattc tttagaaata aagaagttgc tcaaatgata aaaagatgta atgattttgg
     9901 agcaggtgga gtttgtgttg ctataggaga gattgctgat agcttagata taaacttaga
     9961 cttagttcct aaaaaatatg atggacttga tggaactgag cttgcaattt cagagtcaca
    10021 agagcgtatg gcagtagcca taaaaaaaga aaataaagat aaatttatac aattagcagt
    10081 agaagaaaac ttagaagcta cgcatgtagc tacagtaaca gatacaggat atttaagaat
    10141 gttctggaat ggaaaagcaa ttgtagatat aaatagagag tttttagata caaacggagt
    10201 aaaacaaact actgatgttc atgttactaa agtagatgaa gaaaatacat tctttagctc
    10261 taatgaaata gtaaaagatg ttaagtgtgc aagtatgaaa gacaagttta caaaagtact
    10321 atctgacctt aatgtttgtt ctcaaaaagg attagtggag atgtttgata atacaattgg
    10381 tggtaataca gtacttatgc catttggagg aaagtatcaa gcgactccaa cacaaggtat
    10441 ggttgctaag ataccagtac ttggaggaga gactaatact tctacaataa tgacttatgg
    10501 atacaatcca aaggtaggaa aatggagccc attccatggt gccttgtatg cagtagtaga
    10561 atctgtgtgt aaattggttg ctattggagg aaattatagt acaacaagac ttacattcca
    10621 agagtatttt gaaaaacttg gtaacaatcc agaaaaatgg ggtaaaccat tctcagcact
    10681 tttaggagca ttttatgcac aaagtaaatt tgagatacct gctataggtg gaaaagacag
    10741 tatgtctggt acatttaaag atattgaagt tccaccaaca ttggtttcat ttgctgttga
    10801 cacagtagat gcgaagaaag tagtatctcc agaatttaaa aaagcagact caaaagtagt
    10861 aatgttatgt gtaaacaagg cagaaaatga tgttgtagat tttgaggaat taaagagaaa
    10921 cttagataaa gtaagagaac ttatacatgg aaataaagtt ttatcaacat atgcattagg
    10981 atttgcaggt gtaggtgaag caataagtaa aatggctttt ggtaataaaa taggatttaa
    11041 gtttagtgaa gaggctgaaa aagcatttac agatgataaa ttatttgagg ctagttatgg
    11101 aaatatagta cttgaattag caaatgatga tttaagtatg ttagaaggat acaattatgt
    11161 tgtgcttgga agtacagtta aagaagctag tatatttatc aaaggtgaag aacttgcatt
    11221 agatgaatta tataaagcac attgtagtac tttagagcct atattcccaa ctaaaacaga
    11281 agaagtaaaa tcaaagatag aaactataag ttatatatca caaggagaag ctaaaaaatc
    11341 atcattaagt atagctactc caagagtatt tataccagca ttccctggaa caaactgtga
    11401 atatgactct gcaagagcat ttgaaagagc aggagctaat gcaagtataa gagtatttaa
    11461 gaatttaact tataaagaca tagaagattc tatagataca atagtaaacg aaataaaatc
    11521 ttctcaaata ataatgttac caggtggatt tagtgctggt gatgagccag atggttctgg
    11581 taaatttata gctactgtat ttagaaatcc tagagtgcaa gaagctataa atgaattttt
    11641 aactcaaaaa gatggactaa tgttaggtat ctgtaatgga ttccaagtat taattaaatt
    11701 agggttagtt ccatatggag aaataagagt tccaagtgaa agtgctccaa cacttactta
    11761 caataatatt ggtcgccatc aagctaagat tgctagaact agaatttcat ctaataaatc
    11821 tccttggtta gctcaaacta atgttggaga tattcataat atagcaattt ctcatggaga
    11881 aggtaagttt gttgccagtg aagatgttat gagagaatta attgctaatg gacaagtagc
    11941 aactcaatat gttgacttta ataatgaagc tacttatgat attgagttta atccaaatgg
    12001 ttcattctat gctgttgaag gtataactag tgttgatggt agagtgtttg gtaaaatggg
    12061 acattctgaa agaattggtg aggaagtata taagaatatt ataggtgaaa aagaccaaaa
    12121 gatatttgaa tctggagtta aatattttag ataaagcttt gatgtttaat tgatagctag
    12181 aatatataat ttagatattt agatttaaag agatgtaata atatgatgat aatcatttaa
    12241 tattacatct cttttttatt tatctaagaa ataataagtt caacctaagt taaaatacaa
    12301 gctgtgttat aattaattat taagtaaaat tgaatattta ttggagggca acataattaa
    12361 tgaaaatatt aataacaggg tctaatggtc aattaggaaa agaattggtt aaccaattag
    12421 aagcaatcaa tcaaagtata aaccaaccaa aatatgttat attagctaca acaagaagtg
    12481 atttggacat atccaatcag actaatgtag ataattttat tttgcataat aaacctgatg
    12541 tagttgtaaa ctgtgcagca tatacaaagg ttgatgcatg tgaagataat atcgaaattg
    12601 catacaaaat aaatgcttta ggagtgagaa atctagcaat agcatcagaa aaagttaatg
    12661 ctaagcttat tcacatatca actgactatg tattcaatgg tttttctaaa tatccatata
    12721 gggaagacaa taaaactgaa cccaattctg tatatggaaa aagtaaactt atgggagaaa
    12781 aatttgttga acaattctca cataagtact ttatattgag aacagcatgg ttatatggta
    12841 atgggaataa ttttgtaaag accatgataa aactttcttt agaaaataca gaggttaatg
    12901 tggttaatga ccagtttgga actccaacaa gtacagtaga cttagcaaaa attataataa
    12961 aaatcatgga aacagaacat tatggagttt atcatgctac atgtgaaggt gagtgtagtt
    13021 ggtatgattt tgccaaaaag atatttaaac taaaaaatat agatataaaa gtaaatccaa
    13081 ttaagagtaa cgaatttaaa tgtaaggctc caagaccact atattctgta ttggataatt
    13141 ttatgttgaa gttaattggt ttaaattctt ttagaaagtg ggaagaatcc attgaagaat
    13201 atttagaaag atatgatata aatgaaaagt aaatgtatta ttttgagaaa agaaaataag
    13261 atattataaa aaatataaat atttctttta tttatccgat aatcataata tgacaaaaac
    13321 aaatttaagg atggaaaaag atgaaaggaa ttattttagc aggagggtct ggtacaagac
    13381 tatatcctat aacaaaatgt atatcaaaac aaacacttcc aatatacgat aaaccaatga
    13441 tatattaccc tatgtcggtt cttatgttgg cagggataca agatatatta ataatatcta
    13501 caccaagaga tataaaagca tttgaagaat tatttggaga tggaagttca ctagggctta
    13561 acataacata tgcaattcag gagaaagcaa atggaattgc agaagcgttt ataataggag
    13621 aagaatttat aggaaatgac agagtgtctc ttgtattagg ggataatata ttttatggat
    13681 atggatttag tgaaaggttg gagaatgcag ttaaaagaaa aggagcaact atatttgggt
    13741 atcatgtaag taatccaaat tcgtttggag ttgtagaatt tgataaggat tttaatgtat
    13801 tgtcaataga agaaaaacct acagttccta aatctaatta tgctatacca ggattatatt
    13861 tttatgataa tgatgttgta gagatagcaa agctagttga accgtcagat agaggtgaat
    13921 tagaaataac ctctataaac agtgaatatc taaaacgagg aaaactaaaa gtggaacttc
    13981 taggaagagg tatggcttgg cttgatactg gtacacataa aggtctattg gatgcttcta
    14041 attatgttga agtggttcaa acaagacagg gactgtatat agcatgttta gaagagattg
    14101 catatacaaa aggatatata agtagagaac aattaatgaa actagccaag ccactgataa
    14161 aaactgaata tggtcaatat ctattaaaat tggctagtga agatatgtaa tatggtagat
    14221 aaaatttatt ataaatttta ttaagggagt ttattataat gggaaaaatc aatgttataa
    14281 agactaaaat agaaggatta tatatagtcg aaccaaatgt attttatgat gaaagaggat
    14341 attttatgga aagctataat aaaaaggatt ttatagagca gggtcttgat atgtgcttta
    14401 ttcaagataa tgagtctagc tcaagaaaag gcgttttaag aggaatgcat tttcaggtta
    14461 aatatcctca aggaaaatta gtcagagtta cagagggaga agttttcgat gttgcagttg
    14521 atttaaggaa agattcacaa acatttggga aatgggaagg tgtaatattg tctgataaga
    14581 ataaaaaaca attttatatt ccagaagggt ttgcacatgg atttttagtt ttatcagaaa
    14641 aagcagtatt tagttacaaa tgtacaaatt attattaccc agaatttgag aatggcttta
    14701 tatggaatga caagcaaata tctattgagt ggcctttaaa aggaattgaa gaaatatttt
    14761 tatcagaaaa ggacaaaaaa caaaaaagtt ttaaagatat ttgtacaaga cgttaataaa
    14821 aatttattag aattaacatt taggagaaaa aatatgaaaa aaatactgat aactggagga
    14881 gcaggattta ttgggagtaa ttttatacat tatatgctta acaagtatgt agattatttt
    14941 attgttaacc tagacttgtt gacttatgct ggtaatttag agacattaat agaaatacaa
    15001 gacaattcca attataaatt tgttaaagga gatatttcaa atagggagca ggtgtataag
    15061 ctatttgaag aagaaaggtt tgatatagtt gttaactttg ctgctgaatc tcatgtagat
    15121 aggtcagttg agaatcctga tttatttatt aaaacaaaca ttataggaac acaagtactt
    15181 ttagatgcat ctataaaata tggagttaaa aggtatcatc aaatatctac agacgaagtg
    15241 tatggtgatt taccaattga tagaaaggat ttattcttta cagaacaatc ttctatcaat
    15301 ccaagttctc catattcagc ttctaaagca tcagcagatt tattggtaag ttcttattat
    15361 agaacatatg gattgttaac aactatatct aggtgttcca acaattatgg gccatatcat
    15421 tttcctgaaa aattgattcc tcttatgatt ttaaatgctt tggaaaacaa acaattgcca
    15481 gtatatggga atggggaaaa tgtaagagat tggttacatg tatatgacca ttgtactgct
    15541 atagacttaa taatacataa aggaaatata ggggagattt acaatatagg aggtcataat
    15601 gaaagaagta atctagaagt agtaaaaatg atattaaatc ttttaggtaa atctgaagag
    15661 ttaatatcct atgtaaatga tagacctggt catgatttaa gatatgctat tgatgcaact
    15721 aaaatagaga atgagttagg ttggaaggca aaatatgact ttgatttggg tataaaagaa
    15781 actgttaagt ggtatataga aaatgaatct tggtggaaag ctgttttatc agtataactt
    15841 taaaatttag aaaggggcgt tttatattga atgagataag ttcactaatt aatatattag
    15901 cattgactca attatctggt agtaatacta caaatcaatg taattgtaca tataataatt
    15961 catctggatt tgatatgatt atgatgacct tgttaaaagc attatcacaa aataatcaac
    16021 atacttctaa tagctacaat ttattgtcta attctgagaa tttatttaat gaacttgata
    16081 atgtagtaaa taataccact agtagattta tagatgttga tacaaaagat aaaaatgtaa
    16141 agttgagaat tgaaaatgct gtagatcaag catcaaaaaa atataacgta gattcaaatc
    16201 ttataaaagc aataattaaa gttgaatcag attttaatcc aaataccgtt tcttctgctg
    16261 gtgctaaggg tcttatgcaa cttatgccag aaaactgcag agacttagga gttacagacc
    16321 cttttaatat agaacagaat atagatgctg gaactagaca tattaaggaa tacattgata
    16381 tgtttggagg gagcatagaa atgggattga tggcatataa tggtggtcct ggaagaatga
    16441 aaagtagagg agtagaatca atctctgacc tgtataaaat gccaaaagaa acacaaaatt
    16501 atattcctaa ggttatgaaa tattatagag ggtaggcttt attatgggaa taaatataga
    16561 taagctaaac ataagtccaa gtataaatat ggaggttaaa aaactaaaag agagtaataa
    16621 attttctgac aaattaaact ctttaaaaca atcttcttca ttggaagata taaaacgaaa
    16681 tttggaaagt gtaaaaaagg caggagaaag gcttgtactt actcaaaact atggcgatat
    16741 tagtatatat aaaaatgcag taaaagaata cctaaaatct gttgtagata atatgtattc
    16801 acttgataaa aatagtagct tttgggagca tcagtattat aagaatgtaa agattgttga
    16861 tagtaaactt gaggatatga ctagtaagtt aattagtgaa gaaaaagaaa acatagatat
    16921 tgtttctact gttgacatga tacaaggctt attgatagat atgtatagat aaggttatac
    16981 aaggagaaaa attttatgaa tagcaaaaaa gaggatatat atgaattgaa ttttgggcaa
    17041 ttaatagaag aaaaatctgg tgttatagaa gaaaatcaaa atgtttttaa tgcaaaagta
    17101 aatgtagctg tttctatagg aaatacaaca gaaacaataa aaaatatatt agatttaaaa
    17161 gaaggctcta taataaaatt aagtaagaat gtggatgaaa aattagatat atatgcaaac
    17221 gatagattgt ttgcttatgg agaaagcatt attacaaatg agaggttatc tgtaagatta
    17281 tctaaaatag aagatccaga tgtaaattag cattttaaat agtatccctt taggctattc
    17341 tgtatgagtt tagctaaggg gatattttta taaaaaattt aaaatatgtg tgaattatat
    17401 aaatattttt attttatgag cgataactaa attataagta tgtaattttg gaggtgaaac
    17461 tatggatatt aaaaatgtaa gttcaaatat tgtagtgaat aattatttaa agaatacatc
    17521 tggtataaaa aaagtggaag aggttaaaaa aattaataaa acagacaaga tagagttatc
    17581 taaatttgca aaggaaatgg caagtgtgtc aaatatgagt gatgagaata ttagaaataa
    17641 aaaagtcgat gaaataaaaa atgcattaga aaatggaact tacaaaataa aaccaaaaga
    17701 aattgcgaaa aaaatgatag aggatatgcg aggataaagt catgattaca gacttaaaag
    17761 ttataatata tgaagaaaaa aatataatta gtaagatgat ggaattatta aaagaacaat
    17821 ttgattatat agttgaaaaa gacttagaaa atttaaatat gttaaatcct aaactagaag
    17881 atattagcag ggagcttgca tccatagaaa taaagagaag acaacttttt ggtgatgata
    17941 tttcaatgag tgaagttgtt gaaaatagta atgatggata cttaaaagaa atatatacag
    18001 acttgaaaca cattcttgct ttagctaaaa atcaacaaga atcaaatgat tctctaataa
    18061 agaaagaact tatttttacg aaaaaaatga ttaatttcat aaaaccagct gataagcaaa
    18121 ctacaactta taattcttat ggaaatatta gaaaataaat attaggagga gaaatatggc
    18181 aggattattt tccattttaa atacagctaa ttcaggaatg aatgcgcacc aaaaagcaat
    18241 acagacaatt tctcataata taagtaatct agatacagac ggttattcta ggcaaaggac
    18301 cgagtttgcc acaaatagtc caatgtatat gcctagtttg agtaattcaa ttgcaagagg
    18361 acaattaggg actggagtac atgtaacgga tgtaacaaga gctagaaata gtttttatga
    18421 ttaccaattt agggcagaag ctcataagta tggtaagaca gtttcaaaat atgattatta
    18481 caatactata gaaactatac taaatgaacc atcagactat ggaatatctg caggtataga
    18541 tgattttttt aaaggatggg agtcattatc aaaggatata aatagtgaca gtaaaaaaag
    18601 tctagtggta gaaaacgcat ctaatttagc aactttaata agtgaaagtt ataagaagct
    18661 tgataagtta aaagaagata tagattctaa tatagacact gagataaaac aaataaatga
    18721 catgttaaaa agtcttgaag atttaaataa atctattgat attatatctg gttctggctc
    18781 tacaccaaat gatttacttg atgaaagaga tagaatttta gataacttga gttttaaact
    18841 agactttgaa aatagtgatg ttaaaaatat gcttgcagat ggtaaattag aacttaatga
    18901 attaaaaaat ccagatggaa cttggaaaac aggtatatca gggaccttac aaggcttgtt
    18961 tgaaatgcat ggtaagattg atacatataa aagcgattta aaagatgtat ctgatggatt
    19021 agctaaacag ataaatgatg tttataatac gtctgcagga ataacagtaa cagatttttt
    19081 tataacttca aatgtagatg gtgaagatat tataaagata aatccagcta taaagagtaa
    19141 cccaaatgaa ttaaagctta ctgctgatga agcttcaaaa attgctaagt taaaggatga
    19201 aaaaatagat attggggtgg ctggtggaaa agtaagtaca ataagtgacc actataaagc
    19261 ctttgcagaa tcaatcggtt tagattctca aaaagtaaat caagatgaag caaatcaacg
    19321 taaaattata aataatattg ataactcaag aatgagtgtt tcaggtgtat ctttggatga
    19381 agaaatgaca gagcttataa aaatacaacg ttcatatcaa gctagtgcaa aagttatgtc
    19441 aacagctgtg caactattgg atgttgtaat aaatggaata atttaataga agggggaatt
    19501 tataatgaga gtcagtacag gtatgatgtc tagcagctat ctaaactcac tacaagataa
    19561 tctacaaaga ttggataagg ttaatagaca agttaataca acaaaagaaa taaacaagct
    19621 atcagataat ccatataaag caataaagat attaaactca aaaagtgaaa taaagactat
    19681 ggagacttat gttgagaatt gtaaagatac agggaattgg ttagaaacta ctgatactgc
    19741 attagatcag cttggaaaat tattatcaga cataaagaaa actttggtgg caagtggtga
    19801 tggtgcatac tcagatgatg aattaaagac tcttagtaat gaaactaatg aaaaaatgaa
    19861 agaaattgca aatgctttaa atgcaacaca tgaagggaag tacatatttt caggttcaaa
    19921 tacgggggta ccacctgttg aatgtataga taatgctgat ggttcagtga gtttaaagtt
    19981 taattctagt ctaaatctta ataaattaaa tgactctttg agtatggata tagctcaagg
    20041 aataagtgta gattataacg ttaaattgag tagccttgga tttgacccaa caaaaactga
    20101 cccatttgaa gagttaaata acatatcaaa aaaattagca aatccagatg ctgatgctat
    20161 aaaagagctt acaacaactt gtttgggcga tatggaacaa ctaatagaaa atacagtaaa
    20221 tgttaggtct atatatggga caaaagcaaa tacagtagag gctatgaagg aaaaaaacga
    20281 tgatagtttg atacagttaa aggatatttt gtcacaaaat gaagaaatag attatggtga
    20341 aaaacttgtt caattaaagg cagcagagtt aacttatcaa gcttcactac aaacaggagg
    20401 aaagttattt aatgtatcta tattagatta catatagtaa aataacgcag atatgatgaa
    20461 ggttacatta aaaaaaggta tattaggttt tgaaaattta aaagaatatg agcttttaga
    20521 tatagaagat aatgattctt taaaagaatt taattctact gaagaagaag gtataggatt
    20581 tgttgttgta tctccatttg agattatcaa tgaatatgaa atagttttaa atcaggaaac
    20641 aatagaaaaa ctagaagtga aatctccaaa ggatgttgta ctatttaata taattactct
    20701 tggtcagaca tttgaagaat caactgtgaa catgaaagct cctatagtga taaatataaa
    20761 aactaatttt ggaatgcaaa taattttaca ggatgataaa tattcaataa ggcatccatt
    20821 attgagaggt gataatggat gctagtaatt tcaagaaaaa aagatgaagc agtacttata
    20881 ggagaaagca tagaagtaaa aattgtaggg gtagatggaa acaatgtgaa actagctata
    20941 tcggcgccta acaatataag tatattaaga aaagaaatat acgaaaaagt aaagagtgag
    21001 aatataaaag ctacaaataa aaatataaaa attttaaagt cattaaaata ataagcttgg
    21061 aggaagtaat atatgtatgg agaaaatcct tataattctt ataaacaaaa tgctgttttt
    21121 atggcatcaa aggaacaatt gttacttatg cttgttgatg gagcagtaaa atataccaaa
    21181 atagccagag cagctatctt agataaaaat acacagaaag cgcatagaga acttgtaaga
    21241 gttcaggata tatttacaga actaatggtt acattagacc aaaatgcagg acaatgggca
    21301 aaggatatgt acaaagttta tgattttgta agatatgaac tttcaagagc caatattaga
    21361 aaagacatcc aggtaataga tagtgtgttg cctgtaatag aacaaattaa agatacatgg
    21421 catgaagctg ataaaaaaag taaagaagaa agaagtaggg acaaataatg gaattaaatt
    21481 tagctgaatt agataagtta tcaaaagagg aattactatt gatgcttgta gatggcacag
    21541 taaaatatac caatatatcc aaagaagctc ttttaaataa cgactatttg aaagcacata
    21601 atgaacttgt aagagttcaa aatatattta cagaactaat gactacatta gaccaaaatg
    21661 caggacaatg ggcaaaggat atgtataaag tttatgaatt tataaaatct gaattagcaa
    21721 tagctgatga aaataaagat attaaaataa ttgatgatat attgccaata gtaaaacaaa
    21781 ttagagatac atggcatgaa gtttacaatc aactaaaaat ataattagat ggagggataa
    21841 aggtatgtca agtataagtc ctataagagt tacaggtctt tcaggaaatt ttgatatgga
    21901 aggcataatc gaagctagta tgattagaga caaggaaaaa gttgataaag caaaacaaga
    21961 acaacaaatc gttaaatgga agcaagaaat atatagaaat gttatacaag aatcaaaaga
    22021 tctttatgat aaatatctaa gcgtaaattc tcctaatagt atagtaagtg aaaaagcata
    22081 ctcttctaca agaataacca gttctgatga aagtattata gtagcaaaag gttcagctgg
    22141 tgcagaaaaa ataaattatc aatttgcagt ttctcaaatg gctgaaccag caaaatttac
    22201 tattaaacta aattcaaatg aacctattgt tcgacaattc cctccaaatg ctagtggagc
    22261 tagttcttta actataggag atgtaaatat accaatatct gaacaagata ctacaagtac
    22321 tattgtaagt aagataaact cactttgcgc agataatgat ataaaggcta cttatagtga
    22381 gatgacaggt gaattgatta tttcaagaaa acagactggt tcgtcatcag acattaattt
    22441 aagagtaatt ggaaatgaca atttggctca gcaaattgcc aatgataatg gtatcacatt
    22501 tataaatgat gctggtggaa acaaagtggc aagtgtatat ggaaagaatc tagaagctga
    22561 tgtaactgat gaacatggaa gagtaactca tataagtaaa gaacaaaatt catttaatat
    22621 agataatatt gactataatg taaattcaaa aggaactgca aagttgactt ctatcactga
    22681 tactgaagaa gctgttaaaa atatgcaagc atttgtggat gattataata aactgatgga
    22741 caaggtctat ggtttagtta ctactaaaaa gccaaaagat tatccacctc ttacagatgc
    22801 ccaaaaagaa gatatgacaa ctgaagaaat agaaaaatgg gaaaagaaag ctaaagaagg
    22861 tatacttaga aatgatgatg agttaagagg ttttgttgaa gatattcagt ctgcattttt
    22921 tggagatgga aaaaatatta ttgcattaag aaaactaggt atcaatgaaa gtgaaaatta
    22981 caataaaaaa ggtcaaatat catttaatgc agatactttt tcaaaggctc ttatagatga
    23041 tagtgataag gtatacaaaa cactagcagg ttattcttcg aattatgatg ataagggaat
    23101 gtttgaaaag ctaaaagata ttgtatatga atattctgga agttcaactt ctaaacttcc
    23161 taaaaaagca ggtatagaaa aaactgcttc tgctagtgaa aatgtatatt caaaacaaat
    23221 tgcagagcaa gaaagaaata taagcaggtt agttgaaaaa atgaatgata aagagaaaag
    23281 actttatgct aaatattcag ccttagaatc tttgttgaat cagtattctt cccaaatgaa
    23341 ttatttctca caagcacagg gtaattaatt atggataaat taaaagaaaa attaaattta
    23401 tataaagata tatctttaca aattataaat ttgattgaaa aggaagaata tataaagata
    23461 agtattaagt taggtgaaag acaagagatt ataaatagtg taagtgaaat tgatagaaat
    23521 gattttatac agctttataa tagaatggaa ttaattgaaa ttgatagcag gataagagat
    23581 attttacaag gacaattatt agaagtaaaa aaagaattac atgagtataa gcttactaaa
    23641 caagtgaaca caatgtatta taacttaaat agagaaaaag taaatatttt taataaaaaa
    23701 gtataaagtt atgtaccatc ttgtccgatt atataaatga agaattaata aaaaagatta
    23761 aaagtagaaa tgacaaggat gtcaactata ctaaggaggg taaaataatg agagttaata
    23821 caaatgtaag tgctttgata gcaaataacc aaatgggaag aaacgtaaat gcacaaagta
    23881 agtctatgga gaagctatct tctggtgtta gaattaagag agctgctgac gatgctgctg
    23941 ggcttgctat atctgaaaaa atgagagctc aaataaaagg tttagaccaa gcaggaagaa
    24001 acgttcaaga tggtatatct gttgtacaaa cagcagaagg tgcattagaa gaaactggta
    24061 acatactaca aagaatgaga actttatcag tacaatcttc aaatgaaact aatactgctg
    24121 aagaaagaca aaagatagct gatgagttac tacaattaaa agatgaagtt gaaagaatat
    24181 caagctcaat tgagtttaat ggtaagaaat tacttgatgg aagttctaca gaaataagat
    24241 tacaagttgg agcaaacttt ggaacaaatg ttgcaggaac atctaacaac aacaatgaaa
    24301 taaaagttgc tctagtaaat acttcaagta taatgagtaa agcaggtata acaagttcta
    24361 caatagcaag cttaaatgca gatggaactt ctggaacaaa tgcagctaaa caaatggtat
    24421 ctagcttaga tgtagctctt aaagaattaa atacatcaag agcaaaatta ggggcacaac
    24481 aaaatagact agaatcaact caaaacaact taaataatac tatagaaaat gttacagcag
    24541 ctgaatcaag aataagagat acagatgttg cttcagaaat ggttaactta tctaaaatga
    24601 atatattagt tcaagcatca cagtcaatgc ttgctcaagc taatcaacaa ccacaaggag
    24661 ttttacaatt attaggataa aataattttg tatattggat atagaagaaa gggtaaggct
    24721 ttgctttact cctttctttt tttacaaata tacttttgaa aggaaaattt atatgttact
    24781 tagtatagtt atgattgtta aaaatgaaga aaaaatactt gaaaaaactt taaaatcatt
    24841 aaccacatta agaaattcta tagaaagtga acttataata gtcgatacag gttctacaga
    24901 tgatactata aaaatatcta agaagtacac ggaaaaagtg tattttcata attggaataa
    24961 tgatttttct agtatgagaa atatatcaat atcctatgct aaaggtgaat ggttacttat
    25021 attagatgca gatgaaatac taattgattc ttcaactatt gtaaaatttt ttaaagatgg
    25081 tcttgataaa aaatttaatt ctgcttcaat acaattaaaa aatctttact catatgataa
    25141 aaaattatat ggatattgtt cagtgcttag actttttaaa aacgttgatt ttagatacca
    25201 tggtaaagta catgaacaac caatatacaa aaaccctata tttaataatg ttgctgattt
    25261 tgaacattat ggatatttgt ttgaagatga agaaataaga ttaaataagg tgaaaagaaa
    25321 tgaaaaacta ttatttcaag agttaaaaga gaatgataat tctccatata caaactatca
    25381 attagggaaa aattttatta tattaggaaa atatcaggag gctttatact atcttgagaa
    25441 atcttatgaa ttatattcta aactagaatt ggcacctggt tacttgataa caagccttgt
    25501 aaaaacatat ttatatttag gaaaacataa gaagtgtgaa agattgtgct taaagtatat
    25561 taagaaagac atgaataata ttgatatata ttattatatt gcacaagcac aagtagattt
    25621 aggaaagtat gaaaatagta tagattcgta taagagatat atttacttgt tagataatta
    25681 tgaaataagt acacaagcaa atagtttatt ttcagacact gatactgtag ggcttagaga
    25741 tgaggctatt attacactga taaagattta ttataagctt gaaaaatatg agtttgtaat
    25801 ttcagagtac aataacattg aagatgtgga aaagaaaaag aatgtgtact ttagcttatt
    25861 tatgtcccta tataaattga atagatttga agatataaaa aattattata aagaagtttc
    25921 aaattctaaa attgaaaaaa atagttttta tagaaatata gaagaagtta taaataatat
    25981 taaagaggat gaaaaagagt atgtatataa aatcctttgt gacattgaag gtaattatgg
    26041 cattttaaat caaataagga taaataaatg tgtatctata gataaatgta aagaaatact
    26101 aaatgaagaa aaggaaataa tttatgcata tattataaaa atagcttttg aagagaagat
    26161 tgatttacta gatatttttt atgaaatgga atatgtatgg atagagaaat atctaaaata
    26221 tcttttagca ttagaaagaa gttttaattt agtactatat aagtacataa ttagtcgtcc
    26281 aaatacatca gatatggaaa agataagagt atataaagct atatctaaag tagttttaga
    26341 aagtataagc ttaagtgatg aaaaatataa ggagattttt tatttttata taatgtattc
    26401 ttataagtat ataagataca tatatagcaa tttaagtgat tttgaattaa aaaaatttgt
    26461 ttgcaatgat ttagataagt tcactataga atttaagcat ctacttgaca ttaaaaataa
    26521 agtaaataat gctaatgaaa aattagagta tatacatagt atgaggaaat tactaaatga
    26581 gtatcctttt tataataaaa taataaaaat acttataggt gaattagaag aaaatataaa
    26641 agaaagtgaa gaatttaagg atttaaaata tagttttatt attagtatag aaaatatgat
    26701 tgaaactggc aaaataaatg atgcgaaatt attggtagat gactattcaa aaacatttgt
    26761 ggatgatgta aggattttaa atataaaggg aattttatat atgtttgaaa ataagcttga
    26821 taaagctgat tttatgttaa aaaaggcatt atcattagat gtagaaaatg aagatactat
    26881 atataatata gaatacttaa aaagtttaag ataagtcaaa aataggagaa ttgaagaatg
    26941 aatataccaa tagtggttaa tgaaaaatct aaagacataa taaaagattg gcaatataca
    27001 tgtacaaatg aagatataaa atttacaagc ataataatat tgacatataa tcaaatagaa
    27061 tatactaaat tatgtattga aagtattagg aaatttaccc caaagaataa atatgaaata
    27121 ataataattg ataataactc ttctgatgga acagttgagt ggttaaaggg acaatctgat
    27181 ataaaatctg tgtataataa caaaaactta ggatttccaa aaggatgcaa tcaaggtatt
    27241 gagttagcta ctggtgaaaa catattgttg ttaaataatg atgtaatagt tactccaaat
    27301 tggctctata atttgaataa agctttatgg tctcaagata atataggtgc tgtaggacct
    27361 attacgaatg catgttcata ttatcagcaa attgatgttg attataagtc aattgaggac
    27421 atgtttatat ttgcacacaa aataaataaa tctaatatta acctatggaa atatagattg
    27481 aagttagttg gattttgcat gttaataaaa aaaactgttc tggataatgt aggggtttta
    27541 gatgaattat tcacacctgg aaattttgaa gatgatgatt tatcttttag gatgatagag
    27601 tcaggatata agttattatt atgccaggat acttttatac accattttgg aagtgtgtct
    27661 tttaaagaaa atcgtaataa atatacagat ataatgtata ttaatagaag aaaatttgaa
    27721 actaagtggg gatttaatcc agaatatagt ggaatgataa gaacagattt gatttctaaa
    27781 atagatgata gtttggaggc aaatattaat gttttagaaa taggatgcgg tataggggct
    27841 actcttttag aaataaaaaa tatatataaa aatgcatcaa tatatggaat agaaattagt
    27901 gagtcagcag gtaaacttgc aaagtatagt gcagatgtac ttatagcaaa tatagaaaaa
    27961 gtaaaattgg attataaaaa agattttttt gattatataa tattgggaga tgtacttgaa
    28021 catttgattg acccatggaa gatagtcaat gaattgaaac aatatctaaa gaaggatggt
    28081 tatctggtat ctagtatacc aaatattatg catgtttctg ttttaagaaa tcttgtgtta
    28141 ggtaatttta catatgaaga ttcaggaatt ttggacaaga ctcatttaag gttttttaca
    28201 ttaaatgaga taacaaaaat gtttaatgat agtaattaca taattaatag tattacagct
    28261 actagtatgc cactttcgga aattgataat agcttcattg atacaatatg taaggaaagc
    28321 tgtaaagaaa tgagacaaca gtacgaatct tatcaatata tagtaaaagc ctctaaaatc
    28381 ctagatacta aaagatattc tagtattagt atgattaatt taaaatacgc tttaatgaga
    28441 attgataatg aattagatgt agatagtaat ttaagttata tatttgataa ttataatata
    28501 actgaaccat gttttgttga tgatattgaa tatctaattg atacatttgt gataaataaa
    28561 gcaactctat taaatagatt aggagttgag gcatttaaca gaggtctagg agattttagt
    28621 ataaacatgt ttatgacagt attagagatg gatagagatg atattgactc aatatataat
    28681 ataacaagtg tattatgtga attagaagaa tttgatactg cttataattt aattaaaaat
    28741 agtagcgaaa aagtacagag tgaaagtgat atacaagaga tactttgttt tattgagggg
    28801 aagaaatatg aataatgata agatatgttt tataacctgt gtaaatgatg aaaggcttta
    28861 tgaagaatgt cttgtatata taaatagcct agacattcct aaaaacttta gtatagaaac
    28921 agtagcttta agaggagccg attctattgc ctctgcttat aataaagcta tgagagaaac
    28981 agattctaag tataaggtat acttacatca agatactttt ataattaata aaaatttcat
    29041 acatgatatt ttagagatat ttaaaaaaaa tacagatgtc ggaatgattg gtgtagctgg
    29101 agtaaaagat atgcctgtga atggaatttg gtgggattct gataaaaaag cagggaaagt
    29161 atatgatagt catacaggta aaatggatat ttatgaattt agtaactttg aagaatgttt
    29221 taatgaagtt atggctatag atggattaat tatgattact caatatgatg tattttggag
    29281 agaggataag tttgatgggt ggcattttta tgatttgtca cagagtatgg agtttagaca
    29341 aaataaatac aaagttattg taccaaaaca aaataagcct tggtgtatgc atgattctgg
    29401 agtagtaaat ataaataatg gatatgatga atataggata aaatttgtaa aagagtacat
    29461 aaatcttgat ttatatagag aaaattttta tgaatttgat aagaagaaag caagtaccat
    29521 acttaagagt aataagcatg taatggattg tatgaaagag gagcttacag aacaattaag
    29581 attaaagaat tacaaggatg ttttaggtat tgtttctaaa attgctcatt tttctgtttt
    29641 taatcatact ggtttgtatt cttgtgaaga atgtgagaga gcattgctag aatgtgcaaa
    29701 taatttacca gaaatagaaa atacgaaatt atttacaaaa aaagatttaa aaagaagaaa
    29761 agtattacat gttttaagtg aaggatattc aacaggaggt catacaagac tagttaaaaa
    29821 ttggattgaa attgacaaag actctgttca ttcagttgta actacatggc aatcagatac
    29881 actgccagac tggttattaa aagcaataaa tgatagtggt ggcgaagtta tatctttgga
    29941 tttaatttca gatgattatt taaaaagagc tgtagctttg aggaaaatag cttatgactg
    30001 ggctgatata gttattttat atgttcatat gtgggataca atacctgttg ttgcttttgg
    30061 agttgatggg aaaataccta ttttacatgt taatcactat gatagtactt tttggttagg
    30121 gacaagtatt gcagatattg tcattgattt tagcgaaact agtcaattaa ttacattaga
    30181 taaaagaggc atagataaat ctgaaatatt accaatgcca ttaaaagaaa aagaagtatt
    30241 ttcaaaatct gaagttagaa aacgatataa tttagaggat gatgtgacaa taatattgac
    30301 tattgctagt gaaagaaaat acaatagtgt aaatgatatt aattacttta aaattgctaa
    30361 aacattgatt gaaaatacag ataatacaat tattattgtt gtaggagttg atacagagaa
    30421 tctctattgg aaaactttaa gtaatgttac agataataga gttatatgtt ttggaacaac
    30481 agcaaatata gatgattttt ataagatttc ggatatatac ctagagtctt tcatggtagg
    30541 aagtcatact tcaaaattag atgcaatttt atatgattta attcctgtta agtttaaaaa
    30601 tgaagtttca ccaattttta caaatatgtg gaattttata gataagtttg attataagaa
    30661 tataaaagag gttatagaca tgataagaag gtacaatact agtgaatttg acagggataa
    30721 gataaaagca aaacaatcgg aaataaaaca ttttgttata gataaacact gtattgaaat
    30781 aaaaaactat atagacaaaa tatataatga agttaatcat cataaagtta agtatgattt
    30841 atataaaaat gatgaaatcg aggactatca gctttttgta gcattatatg gctatatggt
    30901 aaaaaataaa aacatgtcat aggtggtaaa atgaacaata ttcatatcaa taaatttgaa
    30961 aaaagctgtg aaaaaaactg ggattatttt gtagaaaata aatctataaa tggtacattt
    31021 ctgcaaacta gaaatttcct taattatcat gaggagggaa aattcactga ccactcatta
    31081 ataatatgta aaggtgaaga aatactagca gtcgtacctg catgtgaaac ttatgaagat
    31141 ggtgaaagaa tatttttttc tcatttaggg agtacttttg gtggcattat aataagaagt
    31201 ggttttaatg atattaggca tgtaaatgcc attacaaata agttagaaat atatttacag
    31261 gaacaaaatt tcgataaagt atatttaaaa aatacatcaa gaatattttg taaagaggat
    31321 acttcattac tagaatattt tttatttaag aataattaca tgttttatga tgaattaagt
    31381 atgtttttag aattaagtaa gtataaagaa gatatattag ccaattttaa atacagtaaa
    31441 agaagagatt ttaaatattc tttgaagaat aatcttacct ttaaaagact gtatagtgat
    31501 gaagatatag tttgtttttt taatttactt tctaattgtt tagatagtaa aggagctaaa
    31561 gtaatacatt cagtagaaga acttttggag tttaaaaata atagattaaa agatattgta
    31621 gaattttatg cagtatatat tgatgacatt atgatttgtg ggagcatggt atttaacttt
    31681 caagaaagag tttatcatac tcaatatcta gcatcaaatt cgacatataa taaatattat
    31741 ccaatgatat ttttatacta taatctaata aaagatgctt tagagagaaa ttttaagtat
    31801 ctttcttttg gaattagcac tctaaataga ggcaaatact tagatgaggg tttagctaca
    31861 tttaaagaag gttttggttc aagtgcaatt tgtaacagaa catactataa aaatataaat
    31921 aagtgagaat catgttttat agagttttta gagtatagta aatatctagt actataatat
    31981 tacataaaaa taattagttt ataaagaagg tttgtatatg aaaagattaa tgattttagg
    32041 ctcaattaag tattttgaga atattgttat gtcagctaaa agagagggaa tatacacaat
    32101 tgtttgtgat aatagaattg atactcctgc gaaaaagatt tgtgatgaag caatagatgt
    32161 tgatgtattt gattttgata aaataaagaa tatagctata gataaaaaga tagatggaat
    32221 tttaactgga tttacagatt ctttaatgaa gccatatgtt tatattgcaa atgaactaaa
    32281 tttgccatgt gtgatttctt gcaatcaact tgaatctgta actaataagg ctgttatgaa
    32341 aaattatttt aaaaataatg gaatacctac aactgattat tgtattattg aaagtttgaa
    32401 agatttagaa aaaatacaag gattaaattt tccacttgta ataaaacctg ttgatggtta
    32461 tggttcaaga ggtgtatatt ttgtatcaaa catagaagaa ttaatagaaa aatttgaata
    32521 ttctaaggaa gcctcgcaag atggacaagt aataattgag gaattttatg aaagtgagga
    32581 aatacaagga ctagcttggg ttcataatgg agagtcacat gtattttata ttggagatag
    32641 agagcttgta aatatacata caggaagagc tggaaaacca gatagattaa tttatccatc
    32701 aaagtattgt tttcaatatg aagaagaaat aaaatccata tatcaaaata taacgcaggc
    32761 ttttggcata aaaaatggac ctctttatgt acaaatgtta gtgggagcat atggaataaa
    32821 ggtatctgaa gtaatgccaa gattacctgg aggttgtgac tatatggcta tttctcaaat
    32881 aacagattta gacataggaa gtttatttgc aaactttagt gtgaataatg aagtaaattt
    32941 tgatgaaata aagaaatata atatgcatct atcaaaatgt gtatatgcat taccaatata
    33001 cataagacct ggcacaataa aaaagataaa taatatagat aaaatagaaa acttagatta
    33061 tgtcactcaa tttctattac tagtaaaaga aggagataca ataggattat atgaagatga
    33121 gagacaggat tgtggaagag tttatggagt agcaaataat gtatttgatg caaatataaa
    33181 gaagaaatgt attcatgaaa tgatagaaat acttgatgaa aataatgaaa atatgataga
    33241 aaatttttaa ataagggagt atgatagata tgaagctaat aactcatgaa caaattatgt
    33301 cattaaacat ttctcagaaa caaggatacg agtggataaa agaatccttt ttaacaaaaa
    33361 aagattgtca attaccacca aaaataagca tggtagaaga agggcatgta ttttacaatg
    33421 taatgccgtc cctaaatcat aatcataaga ttggtggaat aaaaatggtt acaagattgc
    33481 cagggagaaa tccaagttta aattcagaga tatttctata taatatggaa gatgctaaat
    33541 taaaggctat tttagatggc aattatatta cagcttttag gactgctgct gttgctgcct
    33601 tggctatcga tacatttgct aaaaaagatt ttaatactat tgctattatg ggtttaggtg
    33661 taactgcttc tgctataatg aatatgcttt catatactct taaagataaa aatataaata
    33721 taaagttact taaatataaa gaccaagcag agtcgtttgt aaaaaagtat gaaaaaaatg
    33781 aacaatttag ttttgaaata tgtgacacac atgaagaact tattgtagat tcagatgtag
    33841 ttatttctac tgtgacatat acagatgaat atttatcaga atttaaatgg tttaaaaaag
    33901 gtgctttatt attacctgta catgttatgg gatttggaaa tgttgattat ctatttgata
    33961 aagtatattt ggatgattat aatcatttga aacatctaga aaatatagat agatataaat
    34021 attcttgtga attttatgat gttttaagta aacaagctaa aggaagagaa aatgatgatg
    34081 agagaataat atcttacaat ataggtttgt caatacatga tatattattt gcaaataaaa
    34141 tttacgaatt gtgtgaaaca ctaagctgtg aagaaataga attaaaagaa ccaaaagaaa
    34201 aatattggat atagtttatt tcattcatat ttacaatagt ttattgtaaa tatgaatatg
    34261 aagtaatata ttgttagaaa attagcatga ataggataga taaatttatg gataaatata
    34321 atttaattag gttcgtatct attggaaacg aatatatagg aaatttagtt gcattagaat
    34381 caaataaagc aatacctttt gatataaaga gagtttatta catatatgat gtaccaaaca
    34441 atgtaaagag aggatttcat gcacacaaaa acttggaaca agtactagtt tgcctaaatg
    34501 gaagtgtgaa gataaaatgt tatgatggtg taaaagagac tatatttaat ttacataaaa
    34561 atgatgttgg attatatata ggaacgggag tgtggcatga gatgtttgat tttgatgaaa
    34621 aaactgtttt aatttctata gcatctgaac attataatga agaagattat attagagact
    34681 ataaagaatt tttaaagtat ttaaataatg tgtagttatt atacaattag tgatgtattt
    34741 tcttaattaa ataaatttaa gatagccaca tagagaatag atagattagg atgtgaattt
    34801 attgttggtt aaatttaata atttattaaa acagttccaa actcataaag aagaatatga
    34861 acagaagatt aaggatgtaa tggaaagtgg atattatatc cttggaaatg aagtaaagca
    34921 ttttgaagaa gaatttgcta actatgtagg tagcaaatat tgtgttggtt tagccagtgg
    34981 tttagatgca ctgataattg cttttagagc attaggaata agcaaaggtg atgaagtaat
    35041 cgtgcaatca aatacatata ttgctagtgt tatgggaatc agtataaatg gagcaacacc
    35101 agtatttgta gaaccaaatg aatattataa tatagattca tctaagatag aagaaaagat
    35161 aaatagcaaa acaaaagcta tattagtagt gcatttatat ggtcaagcaa gtaatatgaa
    35221 agatatacaa gaattatgtg ataagtataa cttgtattta attgaagatt gtgctcaatc
    35281 tcatggagct aaatatgatg gaaaaatgac aggaacattt ggtgatatag gttgttttag
    35341 tttttatcca agtaaaaatt tgggtgcatt tggagatgca ggagctatag taactagtaa
    35401 tgaggaaata gctgataaag tgaaaattct tagaaactat ggctcagaaa agcgatatta
    35461 taataaattt gtcggatata attcaagatt ggacgaaatt caggcaggtt tattaagagt
    35521 aaaacttaaa tatctaaatg aaattgaaat tgaaaaatca aaaatcgtag atagatatct
    35581 taatgaaatt aataatccca aagtcatact tccaaagata gaaaataatt gtactcatgt
    35641 atggcattta tttgttttac aagttgaaaa acgagataca tttatggaat acttaaagaa
    35701 aagtggtata gagtctataa tacattatcc gataccacca catctatctg aagcatatgc
    35761 ttatttggga tataaaaagg ggagttttaa tatagctgag aactattcag ataaagttgt
    35821 tagtatacct ttatttattg gtatggaaga tgaagaagtt agttatataa ttaaaattat
    35881 aaatgaatat taaactactc ataataatat caaaaataaa ttttttatat atcatttatt
    35941 taaatttcgt taattgacat ttttaaatat tgatatattg tacaaataaa attgaaatat
    36001 atggaaaaat atagaattaa gatgagattt gtcttatatt tttataaatt aaaaatattt
    36061 tcaaagagtt tattataaaa ttgtaaacta tagtgtttat aatctttgta attattggta
    36121 attaaattta aaaaataaaa tatttaatta tacacaaaaa agatatttaa aaaatttgtt
    36181 atttattgta tacttaagtt aaaactaaat aggcaaatct agagaaatct agtgacgcaa
    36241 agctataggg actaaggttt atatacataa actatgtcag ccagttgcca aaaagagtcc
    36301 taggtgtatt gtatacctaa gaaaagtcta taatgactgc ctttttggca gtcattttgt
    36361 tatggaaaaa tcactttatt tcagtagaaa atacacataa taacatattt taccaattat
    36421 aacataaaat aaagaaaaat tatctcaaaa agttactatt ttacaaaaaa tgacttcttt
    36481 atttatttaa attaatcgca tttccattac attaatacaa taatgtatca atttagtaag
    36541 aaaactaata tttttttaat gaagtcccat gaaaatataa aaaaatttta atatgtattt
    36601 aaatatttct tcgaaaagtt gctctttttt gtaatatagc aacttcattt tttttattaa
    36661 taaacaaaat agataaaata tattttgagg aggggtgagt gtgaatacat atgacattat
    36721 aaaaatggga ttagatgcaa ctaatctaag aagtcagaca atagcaaata atatagcaaa
    36781 tataaatact gaaggatata aaagacatta tataactttt gaagaaacac taaatgatgc
    36841 tatgcctaaa atagaagaaa aaacagacaa ttctaaaggt ttaagagtag atggtaataa
    36901 tgtagacata gaagaagaaa aagtaaatca agcaatggca acattacaat ataatgcttt
    36961 gataagtttt gcaaatggaa aaatagcaat gaccaaatca ataataagtg ggaggtaatt
    37021 agaagatgag tgtgttcagt ggaatgagaa tatctgctag tggattaaca gcagaaagtt
    37081 tgaaaatgga tatcatatca tccaatgtag ctaatataaa tgctacaaga ggtccaaatg
    37141 gagaagcata taggagaaaa gttgtttcat ttgaagaaaa ctatgataat aaattaggta
    37201 tgctaggagt aaaagttaca tctgttgaag atgataaatc acctcttaaa gaagtatatg
    37261 acccatctca tccagatgca aagccaaatg gatatgtaga atatccaaat gtaaatatat
    37321 tggtagaaat gacagattta ataacagctt cacgagcata tgattctaac gtagatacat
    37381 tgaatgctca gaagtctatg atatcaaaag ctttggaaat aggaaagtag ggataaatta
    37441 tgccaattac acaaattaac aatttaatgc aaggtgcaaa gttagataat ttgattggag
    37501 aagcaaagcc attaactctt aataataaag aagaaaaaaa gaattttgaa gatgtaataa
    37561 tagatactat agataaagtt aattttcaac aagtaacagc agatgatatg aaagaaaaat
    37621 ttataaaagg cgaagatgtt tctatgcatg aagttatgtt aataggtcaa gaagcacaac
    37681 tatctatgca atatttaata gaagttagaa ataagattca tgatgcatat caagaaatga
    37741 ataaggtgca aatttagcct tttgaacagg agtaataatc aatgataacc agttttaaaa
    37801 ataagattaa ggagcaagtt gctaaatcta ggacaaagat aagttcttta aaaaagggaa
    37861 ctaaaatagc attaggtata ggggttattg ctatatgttt agcgctagta tttacagcaa
    37921 tatctgcgag caaaaataag tatgcagtct tattttcaga actagatgct caagatgctg
    37981 cagatatatc agcagagcta gagtctaaaa agatagatac taaggtaaaa ggaagtacga
    38041 tttatgtacc tagaacagaa gttgataagt taaggctgga gttagcgcca agtcttacag
    38101 gtggttctaa aggatttgca ctaatggatg atagttcatc aatagggctt actgatgaag
    38161 aatttcagat taagaaacaa agaatgatac aaggtgaaat agaaaaaaca ataaagagtt
    38221 ttccacaagt ggaagacact agagtacata taacatttgg agagtcatca gtatttgaga
    38281 aggaggctgt tccagggagt gcagcagttt atttgatgct aaaaccaggg acgactttag
    38341 aaaaaaacca aataaagtct gttatggcac ttatatcagg aagcgttaca aatataccag
    38401 agaaaaatgt tcaggttatt gataacaaaa tgaatttatt atctgaaggt attttggagg
    38461 atgaaaatgt tgatgaaaat aatcaaccaa taaatagtgg cattagtgtt gataaaaata
    38521 aggcatctga acaagcattg aataaacaac tagaaaagtc tattttagaa atgctagaac
    38581 cgatatttgg taagggaaag gtgaaagcta cagtaaatgc tgatttaagt tttgatacag
    38641 ctgaaaaaac tgagataaaa attgaccctg ataaagttgc aataaaggag acaaggtcga
    38701 aaaattcatc taaatctgct gcaaataatg ttcaaggtgt agatgctaac atgaataacc
    38761 aaggaaataa caatggtact aatacagaag atagtttaga tgagagtttt gagtatgaaa
    38821 caggtaagat tgaaactcac actatagttg cgcctggaga acttaataga ttgacagctt
    38881 ctgttgcaat agatggaaaa atagctaaag gtgttcaagc tgatgtagag gatattgtta
    38941 ataatgctat aggtatggat ggagcgaggg gagatactct tagcgttgtt tcaatggtat
    39001 ttgacccaga aggtaagaaa gaggctaaac aagagctgga agagattaaa aaagaaggat
    39061 ttaaaaagaa cataataaaa gcagttattg gagtagtagc agtaatagca gttggagcta
    39121 ttggatactt aatatataag aaaagaagtg aacgagaaga tatggattat gaagatgaag
    39181 attatatttc taaatctgat tctgttgata ggaaggataa tcctctatta ggagatgatc
    39241 cagaactgac attagaggaa gctgttaaaa tgtatgcaga agaaaagcca gaccaagtaa
    39301 cagagataat aaagacttgg ttaaatgagt aagggggata gaaagtgtcc acggaaatta
    39361 caaatgttaa tgcgtttgac ttaagtgata taaatgaaag tgataagaat gagaaggtag
    39421 aaggtgaggg tttaaaaaaa gctgctgtat tattaatgac attaggccca aatgtgtcta
    39481 gtgatataat aaaaagcttg cctgataaac aagttcaaaa aataggtttg gaaattgcaa
    39541 atatttcttc aatagcttca aaagaaagaa gagaaatctt aaaagagttt gtagaaataa
    39601 atagggcaaa agattatgtt atagaaggtg ggcttgaata tgcaagagag ttattaagta
    39661 atgctttagg aaatgttaaa gcaaacaaat tacttgaagg tatatcaata aatacaagta
    39721 caaagccttt taaacatatt ataaaactag atgtaaagaa gatattagaa atacttagga
    39781 atgaaagtgc acaaactata gccgttttac ttgcacatat ggaaccagaa aatggagcag
    39841 aagttttatc atacttagat tttgatttac aaaatgaagt agctatgaga ataagctcaa
    39901 tgggttcaat atctccagaa gtaataaagg taattgaaag ttcattagaa gataaattac
    39961 attcaataag tggtgatgat tttaatgatt atggaggggt gtctacctta gttcaaatat
    40021 tgattaattc tgatagaaaa acagaaaaga atattttggg aactattgaa gaacaaaacc
    40081 cggatttagc agctcaaatt aaatctcata tatttgtctt tgaagatatt gtcaagttag
    40141 atgatataag catacaaaaa gtgttaaaag aagtgactat gaaagattta gtatatgcct
    40201 taaaaggtgt agatgatgag atttcagatg ctatatatag aaatcaatct tctagagcag
    40261 cagatgcact taaagaagaa atggaaatgg ttggtgttat aaaggttaca caagttgagg
    40321 cttctcaaca aaagatagct aatatcgtaa gaaaacttga aagagaaggt gccataacta
    40381 ttgtaaggaa ttttggtggt gactttgttg aataataaga ttattaaaag taaaaaagca
    40441 ttatataaag gagtgctaga tttaaccata atagataaag ttagagtggg agaatcaaat
    40501 gtaaataaag aaaatgattg tagatttagg gatgaggtaa taaaagaagc tatagaatct
    40561 gctgagataa aaaagaatga gatattagaa gaagcaaaaa taaatgcaca aaatatagaa
    40621 aagcaagcat atgaaaaagg gtatagtcaa ggtcaaaaaa atggatatga ggatggatat
    40681 aaagagtcat atgatgaata tacccaaaaa gctaaagatg aagcagattt tataaaaaat
    40741 caggcaaatt taatcttatt acaagctaat gagaagatga ctgagtacat aaaagaaaaa
    40801 aataaagaga ttgtaagact tgctattaat atggccagtt ctgttttaaa aaagcatttc
    40861 gaagatgaag aatctatgag tgatctgtta aagcaaatta ttcaagatta tgaaggaagt
    40921 agttcattta tagtaagatg taattctttc tataaagata atatagagaa agagtttgaa
    40981 ttattcaaag aggaaacatc actaaaatca agtgtatttg ttattgcaga tgatagtata
    41041 gataaaggca atgctattat ccaaaaggaa aatggaagaa taatagttgg tattgattgt
    41101 gctatggaaa aactaaaaga agaattgctt ggtgaataaa gtatgtataa aactgatata
    41161 tctatagatt ttgacaaaat aaataagaaa atagaaaatt tagaactcac tatatcagaa
    41221 ggtagagtaa agaagataat aggacttact gtagaagttg aaggaataaa agcttttgtt
    41281 ggggagttat gcgttatata caaccaagtt aataaaccag tgaattgtga agtagtaggt
    41341 tttaaagata aagaagtcat attaatggcg ttgggagaat taacattgat agcacctggt
    41401 tgtaaagtta tttctaaggg gataccttta agtgttatgt gtagtgataa tttactaggt
    41461 aaagtacttg atggtcttgg aaatcctata gacaactcag atgctgtttt aggagataga
    41521 tacaacctga acaatgaacc tcctgatcca atgaagagaa aaaagatacg aaatataatg
    41581 gaaacaggtg taagagcaat agatgcattt actacttgtg gtgaaggaca gagaatcggc
    41641 atatttgcag gaagtggagt aggtaagagt acgactctag gtatgattgc taggaatgca
    41701 aaagcagatg taaatgtaat tgctcttatt ggagaaagag gaagagaagt actagatttt
    41761 attgataaag atttaggtga agagggaatg aaaaaatctg ttgttgtgtg tgcaacatca
    41821 gataaagccc ctctagttag gttaaaaggt gctcttactg ctactgcaat agcagaatat
    41881 tttagagacc aaggcaaaaa agtaatactt atgatggatt cagtaactag atttgctatg
    41941 gcacaaagag aagttggtgt agcaatagga gagccaccag ctcaaaaagg atatacacca
    42001 tctgtatttg caatgttgcc aaaacttatg gagagaacag ggacatcaga taaaggttct
    42061 ataactgcat tttatacagt attagttgat ggagatgact ttaatgagcc aatagcagat
    42121 acaacaagag ggatacttga tggtcatata gttttatcta gagacttggc aaataagaat
    42181 cactatcctt ctatagatgt attaaacagt ttaagtaggc ttatgaatga aattgcttca
    42241 aaagaagaca taaaaatagc gtcttttgca agagacatgc ttgctgagta tagagaagca
    42301 gaagatttga taaatatagg tgcatatgct tcaggtacaa ataaaaaaat agatgaagca
    42361 atctattatc atgagcatat tattaatttt ttaaaacaag ggataaatga aaagagttct
    42421 tttaatgaaa ctataagtag tttgagaaga gtttttgaat aaacaattaa aaaaatattt
    42481 gaataggttg tgatggaatt ggataaaaac tttaagttta gattacagaa agttttagac
    42541 ttaaaagtga aagatgaaga agagataaaa atggaatttg ctaagattca acaaaaaaaa
    42601 atagatatag agactaattt agaaaactta gaaagtaatt atagcaaata ttctataagt
    42661 aaaaataacg atagtattca aaatcaaaaa ataactatta actatttact agctttaaac
    42721 aattctataa tggatttatc agaagagttg gataagtcaa caaatgaact tgaaaaagct
    42781 agaaagcagc ttataagcaa acagatagag agaaaatcat tagaaaaatt aaaagaaaaa
    42841 aagtatggac agtattataa agaagaaaat ttaaaagaac aaaacactaa tgatgagttt
    42901 gcaagtatga gttatttaag gaatagacaa gttctataag aatagtagga agtgaggata
    42961 ttattgtatg aagatacaag cagattctta taatattatt aaagatattg gggatagaaa
    43021 aagttcacct aagaacaaaa atataaaaaa taataaattt gaagataatc tatataaggc
    43081 aacagaccat aaaaaagtta aagatagaag ggaagtaagt aagcaaaata ttagctcaga
    43141 taaaccggaa aatagtgata atgagtgtca tttagatgaa gataaaagta atcacttaga
    43201 agatgaaaat acatatgaaa aagattgtag taagaataat gaagctttgc agcaattact
    43261 gaatctttta aaggataatt ataaaaaaga taaagatata aacactatat tgaataatat
    43321 agaaaaacta agtatatcag atgaattaaa acaagaactt aagcttaata aaaatatatt
    43381 aaataagatt gcaaatcaaa attcagagct aaataaagct aatataaata actctaatga
    43441 tatagaaaat ttagacatag ttaaattaag agaacttttg gaagataatg ctaataaaga
    43501 tattgaagta attactaaaa ctacagaaaa tgataccaaa aatatagatt tgtatagttt
    43561 tgatagcaat agaatggata atcttaactc aaataaacag aaagatgata gctctgatat
    43621 tttggaaaaa ctagcagggg taaatggaaa taagagtggg aattttggac aagtaataaa
    43681 taaaaatgtt gatatgaata aaattaaaaa tgataatgtc aatttaaaac agatagaatc
    43741 aaatgtaatg gatgactcta taaaagctat taagcatatg aaaacaaatg atatacaaga
    43801 attgacaatt aaattaagac caaaagaatt aggagatatg aatatacaat tgttaaaaga
    43861 tggtgaaagt atgagagctg ttgtgactgt tttcaataaa gatgtctttg actctataaa
    43921 taaaaacata gcagatttaa agcaacatct tgaacttact aatgtaaata taaaagatgt
    43981 atcagtgcaa atgcacagcg ataatacaaa tacatctgat acctttgata aagcatttga
    44041 acaacagaat agacaaaata accaagaaaa catgagtaag aagaatacaa atgataagca
    44101 taataatgta gtcatagaag atgacattaa agaaaatact attgatgatg atagagtaga
    44161 tttactagct tagtaagggg gaagtattgc atgtacggaa tagcaacggc tttggcagga
    44221 actaagggaa actataggtc aaataactta agccaacttg aaagaaatat taatgatatt
    44281 gccaataaac aagttagaag caaaggaaat cagacagtaa cagacaatgg gactccaatt
    44341 ataggacaag gtgatgatgc tgataaagac ttgtttatga agatacttgt ggcacagatg
    44401 ggtaatcaag acccaatgaa tccgcaagac ccaacagaat atgtgtcaca attagcacag
    44461 tttgcaactt tagaaaagat gtcagctatg aatgagaagc tagatgtact tttagcctta
    44521 agtaattcca ctctaataaa ctctgcttta agtatggcaa catcattaat tggtaaagaa
    44581 gtagaagtat ctggggaaga gaaagaagat gaaaacattg taggagtagt aaagtccaca
    44641 tatataaaag atggggaagt acacttagaa ttagaagtag atggaagtga caaaactgta
    44701 ggtgttaaat atgaaaaatt acttaaaata ggagaggtag atgaaaattc aggagaagaa
    44761 aatgattctt cagaacacga atctccagaa gaagcataat attaaatgag aaaagaggta
    44821 aattcatgat aaaagcaatg tattcaggtg taagtggaat gaaagcaaat caaacaaaat
    44881 tagatgtaat aggtaacaat gtagccaacg ctggaactac ttcttttaag aaaagttcag
    44941 ctagaataac agattcattt tatcaaactg tactatatgc aagtgctcca acagctgcat
    45001 taggaggaac aaacttagga caagtaggtg taggtagtaa gatatcaagt ataaataaag
    45061 atatggtaca aggaaatgta caaccaacta atagaccatc tgatttaatg atagatggag
    45121 atggatatct tccagtagta agacaaggaa cagttatgta tacgagagat ggttctttta
    45181 atttagatac aggatttaaa gcagctgatg gtgctttgaa gaatattaaa ggtggaagac
    45241 ttgtaaatgg tgatggatat atgttacaag gtatgattta tgatggggaa tataaagctg
    45301 atactggaga atttgttaaa aagggtgatg ctaaaggaaa agagcctatt gtaataccac
    45361 ttcaaagtat aagtcctaag gatggaacag ttcaaaatgt actagagtat aatatatcaa
    45421 aggatggaac agtagaattt ttattgagtg atggtcaaag aactcaattt gtctatgata
    45481 tacctgatgg tgctaaggta ggagatacac ctgctggaat agataaatta aaaggtacaa
    45541 tacaaaaagt gcaaacatat gcattccaaa atccagcagg actagatgca gaaggaggaa
    45601 acttgtttaa accatcagca aactcaggag acccacaaat agcaggagct acaggaacaa
    45661 taatacaagg ggcaatagaa gcttccaatg tagatatagc agaagaattt acagaaatga
    45721 taatagcaag taggtctttc caagcaaaca gtaaaataat atcgacatca gacgagatac
    45781 ttcaagaaat aataaatctt aagagatagt aggtacatat tatgataaga gtaacggatt
    45841 taaacggaag aaaatacata ttaaattcag accttatagt gaggattgat tctgtaccag
    45901 aaagtaggat tctccttaca acaggagaaa aaaaccttgt aaaagagtcg gtagatgaga
    45961 tagtagataa agtaataaag tataaaagaa aaataaatgt aggttatatt aggagcgaaa
    46021 aataatgaaa aaaacagatt cactgacatt tattggacta atagtatcta cagtattagt
    46081 tttagttggt gccgcaaaag gaagtagttc agggttaaag aatttttttg atgtttcatc
    46141 aatattaatc acagtgcttg gttcatttgg agctctaatg attacattta ctatagatga
    46201 tataaaactt ataaaaaatg cattacaata ctcatttaag acaatgagtg tatcaaagtt
    46261 agatttacta gaacaattta aaacactttc taaaaaagct agaaaagaag gtcttttatc
    46321 aatagagagt gatgtaatgg gaatagaaga tccatttatg aagaaagggt tagagctgtg
    46381 tattgatgga ttggaaatca atgagataag gtccatctta gaactagaga tagattctgt
    46441 tgaagataca aataacagat actctaaact atttaaaact tggggaacct ttgcacctgc
    46501 ttttgggatg ttgggaacac taataggttt aatacaaatg ttagctgacc ttacatctcc
    46561 agatttaata gcatctggca tgggaaaggc attaattaca acgttttatg gttctttact
    46621 tgccaatatc gctttgaacc caattgcata taatatagat gagaagactg aaaaagaaat
    46681 ttatgtaaag gaaatgatgt tggaaggtat aataagcata caatctgggg agagcagtat
    46741 agtagttgag gaacgtttag ccacatacct ttcaaacaat gagaagttag aaattatgaa
    46801 atccaataaa aatacagaaa gagcaatgag caatggcgcg taaaaaaaat aagaaaaagg
    46861 atgagataaa tccagatgca tggctggcta cttatgcaga tactataaca ctgattctta
    46921 catttttcgt attattgtat gcaacatcag ttgtagatat ggaaaaattt aaaaacatag
    46981 cagatgcgtt aaaaggtcag ttttctggga caagtatatt tagtacgggt tcaagtacaa
    47041 gtgagaaatc tccaatagac agtgttttag tggataaaaa tgataaatca aatttagaac
    47101 aggatttaaa agataaagtt aataattcta acttacaaaa tagtgtaaca gtcaaagatg
    47161 atagtagagg gattttacta gagttagatg attcaatttt atttgactct gggatttctg
    47221 agttaaaagt agatagtaaa gctactctta ataaagtata tgacatgata aagatgatga
    47281 aaaataagat agttatagaa ggccatactg ataatgtacc tgtaaaatca agtacacatg
    47341 aatccaattg ggatttatct tctagtagag cagtaagtgt tgtaagatac tttgtagaag
    47401 aaaagggcat ggaccctaga ctattttcgg caacaggatg tggagagtat caacctttgg
    47461 tttcaaatga aacagcaaag ggaagggctg agaatagaag agttaatata cttataacta
    47521 ttgactagga ggaagaggtt tgttcaaaaa taaaaaaatt attataatta ttttgatact
    47581 gattattttc tcagcgggag caggtttttt tatattagga gaaggtggaa ataaacaagt
    47641 tgacaagatt gcaggtttgt ttattccaac aggaaaaggt aaagatgaag taaagaccga
    47701 aaatcttgac ttagaagaga tggtattaaa gctagctgat gaaggtgtaa gatatttaaa
    47761 agtatcaata actgtatctt atgattcttc atataaagat atagaaaaaa atacgccttt
    47821 aattagggat aatattataa attgttttat ggcaaaaagg gcagatgatt ttactgctga
    47881 taatttaaag aacataaaag aagaaattaa aaatacatta aatatacaac taggtgaaaa
    47941 tgtaattcaa aatatatatt tcacaaatat agtagtgcaa taaaaggatg gtaagacatg
    48001 atactttatt tactaaaaat attaatatgt atcccaataa ttttaatatt gatagtctta
    48061 tctttaaaaa ttagccaagg aactttatta aaaaaatata atgataaata tgtaaaaata
    48121 ctagatattg taaatataag taaaaacaat agcataataa ttttaaaaat aggtgaagaa
    48181 ggatgcatta tggcatcttc ttcttctagc atgagtaaaa taaaagattt gaccaaagaa
    48241 gaaatattaa aaatagaaaa cgacattaaa gaaagaaatt ttaaaattca agattttaaa
    48301 atggaaagaa ttaaaaagaa atatgatttc agtagatata ttggaaaatt tacaaaaaag
    48361 gaagataagt aaatggaact attatcaaaa ttacctttgg ctgatgtacc atatacaagt
    48421 tcaatgcaat tgtttttatt tctaactgca ttaatgtttt taccatttgt aatgttcatg
    48481 atgacaagct ttgtcagaat agttataagt ttatcatttt tgaaatcagc attaggagca
    48541 cagcaagcaa ttccaagtca aattttggta ggacttgcaa tagtattgac aatatttata
    48601 atgagaccag tattaaatga gataaacgaa aaggcattac aaccatatat gaaagaagaa
    48661 gttactatgg aagaagctat gaaggaagca gaaggaccta ttaaagagtt tttattaaca
    48721 caaaccagac agacagattt agatttattt gtagaacagg ctggtttaaa agaaaagaaa
    48781 ttaactcgtg aaaatatacc actatctgtt gtagttccag catttgctat aagcgaacta
    48841 aaaactgctt ttcaaatagg ttttttaata tatataccat ttttgataat agaccttgtt
    48901 gtggcaagtg ttttgatgtc tatgggtatg tttatgttgc ctccagtcat gatatcatta
    48961 ccatttaagt tattattatt tgtaatggtg gatggatgga acttgatagt aaaaacattg
    49021 atattgggat ttggatagga gaaaaatagt atgagtgaat ttatgataat gagtgttctt
    49081 aaaaatggca tgtttacagg agctatgatt gcagctccag tactcattgt ttctcttgta
    49141 gttgggcttt taataagtgt gtttcaagct acaactcaaa tccaggagca aactttgact
    49201 tttgtaccaa aactaatagc tattctttta gtagtagcta ttggtggaag ctggatgtta
    49261 catactctta ttagctttac ttctgagata ttcaatatga tatctcttat aactaaataa
    49321 gtaggataca tatgattttt gtatttatta gaatagtagc attctttgga acactaagaa
    49381 tactatttcc agctggtaca cctgctttat ttaagtcact ctttgcaatc attatatcaa
    49441 tattgatttc aagtactatg caaattgaat atgcaacaaa tatagacaac attgtattgt
    49501 ttaccttata tggtataaat gagaccataa ctgggattat gcttggatat ataaccaacc
    49561 tatgttttta tagtatcaga atggcgggtt ctatgatgga ccaacagatg ggattatcaa
    49621 tgattaatat gtttgaccca aattcaatga ctcagacaac tttaattgat aatcttatga
    49681 actggacagc acttatgata ttttttagca tggatggtca tcatgtttta attagaggta
    49741 taaggtatag ttttgagtta attccaatag gaaaaccatt tgtagataat aacatagatt
    49801 atataataaa catatttgtg cagtgctttt taactggatt caaaatagca atacctattg
    49861 tactttgtct gcttatggca gattttattt tagggcttat atcaagaagt gtacctcaat
    49921 taaatgtaat gatagttggt atgccattaa aaatattggt tggtattgca ttatttatca
    49981 tatcaatacc tttaattgtc aatcaaataa gtcatttatt aagtcaaata ccaaagatgt
    50041 atgagggaac ttttgcacta gctcctatgt tttttatggg ttcaacagat aaaacagaag
    50101 aagctactcc aaagaaaaag ggagaacaaa gaaagaaagg taatatagca aagagtagag
    50161 aattacccgt tgcaatgact ctacttgcat tcaccttatt agtacctaca ttattttcat
    50221 atgtagtaga tacacttaaa tcttcgctta attatttttt gagtctagat ttttatatga
    50281 acataaatta ttcaaaccta gagaaattag ttatagcagg attaatggat ttttttaaga
    50341 tatttttacc aatagctatt ccctttttag ttttaggcat aatagcaaac ctatttcagg
    50401 tgggaatact gtttactggt gaaactttaa aaccaaattt atcaaaatta aatccagtta
    50461 gtggattcaa aaacatgttt tctatgagaa gtttgtcaac cttaataaag gatatagcaa
    50521 ttatatctat tttggcttat ataggatata cattcttcca agataattat ttagatatat
    50581 taaagctagg taacatatac ctaccaactt taatgtacac agttaaagat ttagtatatt
    50641 caatattgag taagatttgt gtagctatga tagctatagc agttgcagat tatgtttatc
    50701 aaagatacag tcataaaaag caactgagaa tgacaaaaca agaagttaaa gatgaatata
    50761 aaaactcaga aggagaccca gaagtaaaag ctaagataaa acaaaaacaa agacaaattt
    50821 cgtctcaaag gacaatgcaa gcagtaccaa gtgctactgt aatagtaacc aatccaaccc
    50881 acttatctat agcagtgaga tatgaaaaag ggaaagacca agctccagta gtggtggcaa
    50941 aaggtgcaga ttatttagct tttaaaataa gggaaattgc aaagggaaat gacatcccta
    51001 taatagaaaa caaacctatt gctaggttgt tatataagca agtagaaata gaccaagaaa
    51061 ttccagagga catgtatcaa gcttttgcag agatattagt ggcagtatat aaaatcaaaa
    51121 atagatacaa agtccccaaa aggtaggata agtaatgaat aagtttaatt taaaggaaaa
    51181 tatggacgta atagtggcat ttggtgttgt agggattgtt cttatgataa tagtgccatt
    51241 acctacattt atattagata ttttactggc aattaacata agtttatctg tgctaatttt
    51301 actaatgaca ttatttacaa caaatgtact tgatttatct atatttccaa cagtgttact
    51361 tgtgacaaca ctatttagat tgggattaaa tctttcatca actagattga ttttaacaca
    51421 gggatatgca ggtgaagtta tagaggcatt tggaagtttt gttgtaggtg gaaattacgt
    51481 agtagggata attatattct taattatatt aattgttcaa tttgttgtta taaccaatgg
    51541 ttctggaaga gtatccgaag taacagctag atttacatta gatgctatgc caggtaagca
    51601 gatgagtata gatgcagatt taaatgcagg agttatagat gacaaaactg ctagaaaaag
    51661 aagaaaagag cttcaacaag aagcagattt ttatggttct atggatggag caaataagtt
    51721 tgtaaaggga gatgccatag caggtcttat agtaacggca ataaacataa taggtggaat
    51781 tgtaatagga gctgtaatgc tgaatatgac tcttatgggt gcagcacaga catatgtaag
    51841 acttactgta ggagatggtc ttgtaagtca gatacctgct ctattaatat ctacatcagc
    51901 aggtataatc gtaacaagag cttctactga tgaaaacttt ggtggattag taggtaaaca
    51961 gttaacagca ataccaaaag cagtaatgat gactggctgt gttctaatcg tacttggtat
    52021 gatgcctgga ttacctaaac cagcattttt ttcactaggt ataggtgcta tagcagcagg
    52081 ttacttttta ggaagagaaa agaaagatac tttagaagac ttggagtttg aaaaacctga
    52141 tatggtaaat ccagataaaa atgaagtaga agatgtaaca aacttaataa atgtagaatc
    52201 aatagaagta gaaattggat atggtttaat atctttagct gatgaatcag caggaggaga
    52261 tttacttcag agaatagcat caataagaag acaatgtgca atagacatgg gtatagtcgt
    52321 acaacctata agaataagag ataatttaca gttaaatcca aaccaataca atataaaaat
    52381 aaaagggaat gtagttggtg ggtatgagat aatgcctaat atggttttat gtatggatcc
    52441 tatgaatcaa ggatttagta tatttggaat aaagacaatt gagcctactt ttggaataga
    52501 ggcactttgg atagaaagtg acaaggtaga agatgcagag ttaaagggtt atacagttgt
    52561 agattcatcc acaatactta taactcactt actagatata ataaaatcaa atgcacacga
    52621 attactggga agacaagaag ttaagactat tatagatgca gcaagagaaa attatagtgc
    52681 tgtagtagat gaattgatac cagatttgat gacacttggt gaaattcaaa aagtacttaa
    52741 aaatctgtta agagaaaaag taaatataaa ggatagagtt acaatacttg aaacgcttgc
    52801 agaccagtct agaaatacaa aagatataga gcttcttact gaatatgtaa gaatagcctt
    52861 ggctagaagt atttgtaata atttagttga tgatgataag acaattgtag tggctacatt
    52921 gtctccagaa actgaagagt tgatatctaa taatttacag aggtctgtaa atggaactta
    52981 tccagcagtt gacccagaaa atacaaataa aatatttgaa agtatacaag aagtaatgag
    53041 caatgtatat ttcaatcata atataccagt aatagtagta tcaccaaaga taagagctcc
    53101 atttagaaaa ttagttgaaa ttgtatatcc taatttgact gtattatcat taaatgaaat
    53161 acctaacgat attaaaatca aagcagaagg tgtggtgagc atatatgatg acgaaaaagt
    53221 atacagctaa cacaattcag gatgctatga atctagccaa aatggaattg ggtgacaata
    53281 ttacattaat agataaaaaa gaagtaagaa agtctggaat acaaggtatc ttttcaaaga
    53341 aagatattga actaactatt ggatgggaaa agagagaaaa catagaacaa aaagatttaa
    53401 aacgagagat agaacaatta aagttaatta ttaacaatat ggggtttgat aataagaatg
    53461 ataatgatat agataaaata tgtaaaaatc tactaagttt agaattgaat gaagaaattg
    53521 tagaatctat aagaattgat ttgcaagaaa tgaagttcaa tggaatcgat acaagtaaaa
    53581 atttagtaga aattttaaag aaaaagatta aaatagaaaa tcaagtaata aatggtaaaa
    53641 ttgctctagt cggaccacca ggagtaggta aaacgactac aatagcaaag ttagccgcaa
    53701 aattagtttt tgaagaaaat aaaaaagtag gagtaataac tatagataca tacaggattg
    53761 gtgctgtaga acagttgaag atatatactg atataatgaa tattcccttt aaaggggtaa
    53821 taagtcctga tgaaatggaa ttggcacttg acgagatgaa agattgtgat gttgtattaa
    53881 tagatacaac aggtagaggc tataaaaact ctatgcaaat actagaaatc aaaaatctta
    53941 tagataaagc tgaaactgat aatatacatt tagttttgaa ttgtacaaca agagaaagtg
    54001 atacaaaagc aattatagat tcatatagaa atgtgaattt taaaagctta ataattacaa
    54061 agctagatga aacaataaca tatggatcaa tatttaacat aatgaattat gcacaaaaac
    54121 caatatcata tattacaaca ggacaaaatg ttcctgatga tattataaaa cctaatgaag
    54181 ataagattat aaggttacta ttaggagttg aaagtatatg atagaccaag ctcaaatttt
    54241 gagaaaaatg gcaatggaaa aaagaggact tgatgagttt atagtagaga atgagaatgt
    54301 acctaagata attacaatag cttctggtaa aggcggtgtt ggaaagagca atctcgctac
    54361 aaatttgtct atatgcctga ccaaacttaa taaaaaagtt cttatattag atgcagacat
    54421 aggaatgtct aatatagata taataatggg ggtaaatgta aaagggacta taattgatgt
    54481 aattaatggc gaaaaaaata tagaagatat aatttcacag actaagtatg gtgtaaatat
    54541 aatatctgga ggttctgccc ttaatcatat agaagacttt acagaagctc aaagaaataa
    54601 gtttatacat agcatagaac agattcacga tgtggatttt ataataattg atacaggagc
    54661 tggtatgagc aaaagcttat tgtcatttat atactgtagt actgaatttt tcttaataac
    54721 aactccagaa ccgacatcat tgactgacgc atatagtttg ttaaaagcta taagtaattt
    54781 tggcataaag agaagtgcaa atataataat caatagggta attgatttag aagaagcaaa
    54841 atctacatac aaaagaataa aaatggttgt ggacaaattt ctaaacctta atctagaatt
    54901 gtatggatat ataatagatg acaaaaaagt aagtttatgt gtaaaaaaac agataccatt
    54961 cattttagaa tatcctacaa gtattgcatc aaaatgtatt atagccatag caaagagaat
    55021 ggtaagtcag tcaagagaag atattgttga taataaaagt tttctaagaa agatgtttag
    55081 tatatttaaa agctaaggag gcgtagttaa tgaatagaga agaattaata aaagagaata
    55141 tgcctcttgt aaagagtata gcaagaaaat tttttatacc agggaaaggt tttgaatatc
    55201 aagacttagt aaatagtgga gtcattggat tgattgatgc aataaataaa tttgatgctg
    55261 aaaaaggggc aaagttttct agttactctt atataaaaat aaaatctgct atattagatg
    55321 aaattagaaa tcaaagtcca atttctaaac ataatctaac taaagtaaat aagtataata
    55381 gagtggtaga aaagttgcaa tctaaattat taagagagcc aacttcatat gaaattgcaa
    55441 aagaattaaa ggtttcagaa aaagaacttc atgatatcga aagtaatata gatatgttaa
    55501 atattgtatc attaaactat gtaatctttg aagatacaaa tgaaactgta caagatgtaa
    55561 taagtgatag agaagaggaa gctccagaaa atataataga agaagaagaa aagttagaga
    55621 ttctatccaa agcaattagt aatttaaatg aaagagaaaa attaatactt tcactgtact
    55681 attatgaaga tttaaatcta aaggagatag ggaaagtgct aggtgtttca gaatcaagag
    55741 tttcacaact ccacagaaaa tcaataagaa acttaagaaa caagataaaa gaacttaaat
    55801 attctatata gatggtgatt gataagatga tacaaattat attaatagta gtgtctattt
    55861 taataatagt aactatagtt ataaatttac ataaggaatc aaaaaataaa gataacagat
    55921 actttgataa aatatttgag caagaatatg aagcaaatga tagattaata attgcagaaa
    55981 aaagaagatt ctttttagac aaagtacttg cagaaaaaag aattaatagt catataaaag
    56041 tttatgaaaa ttataaccta ataaaggcta atgaagaaag aaaggaaaac atatttatag
    56101 aatcaaaaaa ggaagtaaga caagaaaatg agttatctct taaagtgaaa catcttataa
    56161 gttcaggtta tgatgatgtg gaaatatgta agatgctaga tattggaaag ggggaactgt
    56221 tattaataag gagttgttac aaaatataag gatattatta tgtgataaaa aagtattctt
    56281 aggaataggt attggtatat taatatcaac tttatgtata atgcttttta gtaataatga
    56341 tatgagtaag gcagagatag aaaaaaaggc cagagcgtta ggcatgaaat atacaagtga
    56401 gatgaaagtt ttagaataga tgatattgtt tagacattta caaaaaagga ggtcataatg
    56461 tgataagagg attatataca gcagtttctg ctatgataac taatcaacaa aagcaaaatg
    56521 ttgttgtcaa taacctagct aatatggata caaatggata taaaagtaaa caacttttga
    56581 ctaaaagttt tgatgaatta aagttggagg gttacaataa ctacgcaaat ggaactaaac
    56641 aaaagcagat tataggcgat ataagtccag gtatttctat ggatgaaact atcacaaatt
    56701 ttaatcaagg acctataaaa actactgata ataaaatgga tgtagctatt caaggtaagg
    56761 gattttttct agttagtgat gcagctggaa atcaatttta cactagaaat gggaacttca
    56821 gaacagacaa tcaaggtgac cttataacaa gtgaagggta ttatgtactt gggacgaata
    56881 ctgctactgg tgctgtaggt catataaatg taggaaatca aaaatttgaa gtggcaaaag
    56941 ataatacaat atcactagac ggaaatgcta tgtataaatt taatattgta gattttaata
    57001 actacaataa cttaaaaaaa gaaggagata acttatattc tggaggagga gctatgaaag
    57061 taaataatgc acttacaata caaagtgcga tagagtcctc taatgtagat atggtatctg
    57121 aaattaataa tatgatgaca atatcaagag aatatgaggc aaatcaaaag ataattcagg
    57181 ctatggattc taaattagca aaaatagcaa gtgagattgg ttcagtaaga taggtaatag
    57241 gaggtgaatt tttatgtaca caatgtttta tactagtaaa acagctttat cagcaaatca
    57301 aagtaaacta gatataatat ctaacaatat agcaaattct gattcaacag gatataaaaa
    57361 aataaatatg gaatattctg atttagtgga tgaagtacta aatagaccat catatcctac
    57421 aaatggcaat gatatatcga ctggaacagg agctaaggca acagctccca tgagaaatta
    57481 tgaacaaggt gcattggctc ctacagatag taagagtaat ttggcaatag atggagaagg
    57541 attctttaga gtgataagaa aagatggtac atatgcatat acaagaaatg gtggatttaa
    57601 tgtagatgct ttaggtaaga ttgtagatga taatggaaat atattagatg tccaatttga
    57661 tgcaggatat aactataata atacaaatat agatagtaca aacctgacag tttcaagaaa
    57721 tggagagcta ttcgtagata ataaaaaaat aggaaaaatc aacttatatc aacctatagg
    57781 aactcaaaat tttatatctg aaggagatag tttattcgtc gcaactgatg cagcacagat
    57841 taaacaagtt gagaaagtcg atatagttca aggctataga gagaggtcaa atgtttcttt
    57901 acaagaagaa tttgttgact taatagccac tcaaagagca tttcaaatga actcaaaggg
    57961 aataaagaca gcagatgaaa tgtggcaaat agcgaataat ttaagagcta aataattatg
    58021 taaggtgagg aatattcatg aatgatatga aagagtacga ttttggcaaa cctcagagtt
    58081 attcaagaga aaatttaact tgtttaaatt ttttattagc tgaattttgt aaaaaatata
    58141 aaaactatat aatttatgaa cttaagtgta attctagtat ggttgttgat agtatagacc
    58201 aagtaaatta tcagactttt ttagatagag taacttcttc atgcgtagta gtacaaaatg
    58261 caatggaacc tgttatgaaa aacttttctt ttagaataga tagaagtgta gctgatatgt
    58321 ggattgatat aacttctgga ggaactggag ttgtaaagga taatgataga gaacttacag
    58381 aacttgataa aaaagtgctt ttacacctta tggatgattt agtaagaaat atgcacttat
    58441 ttgagggttt tgaaaatata caagtattgg atacacatac acatattaat ttgccacaat
    58501 tatgttctcc aactgcacca gtatgcgttg tagatttaaa agtgctaaat gataatgagt
    58561 atattggaag tgtttctctt tgctttccat acaatggttt agaggctctg ttagaaagta
    58621 tatctgttat agaatttttt gataatgatg taggagatga ttcagaggaa tttacccaaa
    58681 aaatctataa tagtgtatct aatatagaat tatcagttat tgcagagata ggaaaagtat
    58741 caattaatgt tgaggattta ctcaaactag aagttggaga tgtaatagta acaaataaaa
    58801 aaataaatga ttatatagat atatttgtag aaaattcaaa atcatacaca gcaacaccag
    58861 gatttatatc aagtaaaaaa ggtgtaaaaa taaatgatgc tgttggaaaa gaggtgtagt
    58921 tatggacaat agtaatctta tttcagatga agaagcaaag atgttattaa atgataaagt
    58981 tgaaagtgaa gttgatggag atgatgagtt agccttagat gtctctgaag ttaatcgaaa
    59041 taatatacaa aggatacttg accttaagtt agaattatct gtagttatag gaagaactaa
    59101 gatgactcta agagatattt taaatttaca taaaggttct cttattgaac tggatacttt
    59161 ggcagaccaa gatgtggaaa tactaataga taataaggtt ttggcttatg gaaaagtagt
    59221 ggtggtagat ttaaattttg gtgtaaagat aacaagtata gtaagtgaag aagacatggt
    59281 aaaatctctt gtttagagat aacatcaatt aatacttaag gcatgaggtg gtacttattt
    59341 taaacttaaa tgatataaaa aaagatatac taaaactcaa taaaaaagac tcatgttctc
    59401 tatataatga atctattaat aatataaata ataattgtat atcaactgga ttaaaatttt
    59461 taaaacaagc aagagattta aacccagatg atgttgatat attaaatctc acaggtcttg
    59521 taaacttact aaaatgtaat tttgatgatg ctgtagaaag tttctataag agttcacttt
    59581 gtgaaaaaac tgctctgtgt gaaaagtata taaacatgct tacatctgag gagtttttaa
    59641 ttttctttga aaagtacaat caacttataa ggttaataaa taaaggaatg tataaagaag
    59701 ctatacaagg gtttaaactt ataactgacc aatactgtga tttaatagaa ccttatgagt
    59761 tgcttgcctt actgtatgac aaggaagaag aatatataaa atttgatgaa tgtttagaag
    59821 tattgaagat tattgataag gaaaattatt tattaaatca agaaaataac acattatata
    59881 tgtataatag tgctaatata caaactaaaa gtaatataaa agtagatgat tataagaatg
    59941 agttcttaca taaaagtagt agtaaccata aaattaatga ttataatgat gtaactaaag
    60001 aaaagagttc aagcaaaaac cagaaaagtt cagattctaa taagaaaaaa agtattaaaa
    60061 aaaataagta cctatatata gccataatat taggaatatt attagtagga caaagtatat
    60121 atagttctta taagatgaat aatttaactg ataaaatatc taaaaataat gaaactaaaa
    60181 atgaagagtc aatagattct aacttgacta agaaagaaaa taatatagaa gaaaatgaag
    60241 atatgagtaa ggcttctaat ataaataaga gtaataaaaa cattaataaa gaggatgatg
    60301 taaaatctaa aaaaaatact gtagaaaatg aagatgataa agattctgac ttatttacag
    60361 aggatgaatt gatgtctaag gcgaatactt taaaatcaga aaagaaaact aaatcttcta
    60421 tacaactata taagaaagtt gcagatatag gtgaaaataa agggaataca tctgaagcaa
    60481 cttatcaagt tgctattctc agtgaaaagt taaaagacta taaaactgct gaagaatatt
    60541 ataaaatgta tgtagaaaat tactcagaaa aagatgctta ttttgatgaa tcttattata
    60601 atttaggtat gatgtactat aacaatggtg atttaaaaaa ttctaaattg acattaaaga
    60661 agttggttaa taaggtacca aatagtatgt ataataattc aaaagtaaaa gaaattttaa
    60721 aagaagaata gttaaattag ataagaaaaa taaattattt tattttaaaa taatatgatt
    60781 ataatatatt gacataccat tttaaaaaat ttaaaatatt aattataaaa tgaggaggga
    60841 taaaaaatgg aatttgaaaa agggagtatt tcaattcata cagaaaatat ttttcctata
    60901 attaaaaaat ggttatactc tgacaaagac atatttataa gagagcttat aagtaatgga
    60961 tgtgatgcag taagtaaaca taaaagactt gtatcactag gagaaatatc agaaaataag
    61021 tcatcagatt ataaaattac agtatctgta aataaaggag aaggtacact taaatttatt
    61081 gacaatggaa ttggtatgac tgaagaagag ataaaaaaat acataaatca agttgctttt
    61141 tcaggtgctg aagatttctt taataaatat aaggataaaa tggaagagtc aaatgatata
    61201 ataggtcatt ttggactagg attttattct gcatttatgg tgtctaaaaa ggtacaaata
    61261 gatacacttt catatacaga aggagcgact cctgtaagat ggatatctga aggtggtaca
    61321 gaatacgaaa tatctgaatc agatgctaga aatgatagag gaactactat aacactattt
    61381 attgatgatg atagtaaaga atttttagat gaatttactg taagaggaat aataaataaa
    61441 tactgttctt tcttaccagt agaaatatat ttagaagatg ttgaaagatt agaaagagaa
    61501 gctaaagaag cagaagaaaa agctaaaaaa caaaaggaag atggaaaaga agagattgta
    61561 gatgcaaaag taattgagcc tttaaacgac actaatcctc tatggttaaa atctccaaaa
    61621 gactgtactg atgaggagta taaagagttt tatagaaagg tattcaatgt atttgatgaa
    61681 cctttatttt ggatacattt aaatgtagat tatcctttca atttaaaggg tatactttac
    61741 ttccctaaat taaaaaatga gtttgaactg actgaaggaa aagtaaaatt atacaacaat
    61801 caagtgtttg tggctgacaa tataaaagaa gttataccag aatttttact tttattaaaa
    61861 ggagttatag actgcccaga cttaccatta aatgtatcaa gaagtttctt acaaaatgac
    61921 agggatgtaa gcaaaatttc taaacatata attaaaaaag tagctgataa gttgaaatct
    61981 ctttgtaaaa atgaaagaga agaatacaat aagttttggg atgatataca aatatttatt
    62041 aaatatggtt gcttaaaaga tgaaagtttc tatgagaagg taaaagaatg tatattattc
    62101 aaaactattg atgatgagta tataacatta caagattatt tagaaaaatg taaggacaaa
    62161 catgaaaata aggtatttta tgtaagtgat aaggaacagc aatctcaata cattaaacta
    62221 tttaaagaat acgacttaag tgctgttgtt ttaaactcat caatagatac tcattttatt
    62281 tcatttatgg aatataaaga gaatggagtt aagtttaata gaatagatgc agacctttct
    62341 gatgtattaa aagataaaaa tgaaaataaa gatagtgaag aaaacaaaga agagatagct
    62401 aagatagaag gtttgtttaa agaagctgtc ggagaaagag tgaagaatta ttctgtagaa
    62461 ggattgaaaa atgaagacac tccagctatg gtattagtat cagaacaatc tattagaatg
    62521 gcagaaatgc agtcaagatt tgctggaatg gatttaggaa tgaattttga ggaagaaaaa
    62581 actcttgtaa taaatgagaa cagtccaata atcaaaaaat tggtttctct taaagatgat
    62641 gaggaaaaga aagataagat tacgcttatt tgtaaccaga tagcagattt agctttactt
    62701 tctaataaag aattaaagcc agatgagtta gattcatttg tacaaagaag taataagctt
    62761 atgagtcttt tgatagaatt gtaagattga atttgaatac attgaagcaa atttataaaa
    62821 agtattcaat atcagtgatg agaaagaagt gtctcaaaat gaacttttca gttcatgagg
    62881 cgcttttttt atgtgaaatt aaatttattt taatcatttc aacaatgaaa aatatattta
    62941 tctctatttt gagacagacc gttctttatt tttttatctt tggttaattt ttaaatattt
    63001 aagtaggttc taataaaaaa ctctatttag ttaaatatag ttaacttgat atataataag
    63061 ataatacata taattaagta aaagattgtt ttttagttta tgtttaaata cataattaca
    63121 ctttgttaaa aacataaaat tattactaaa gtagaaatag gaggttttag catgaatttt
    63181 aattataatt tgccagtaaa tatcttattt gggagaggca gaattaatga tgtgggaaaa
    63241 gaagtatcta aatatggcaa aaaagtattg atagtaacag gtaaaaatag tacaaagaaa
    63301 actggattac ttgataaaac aatagattta ctaaaagact ctaaaataga atatgaagtt
    63361 tttgatagag tagaacaaaa tcctcttata actactatat attcaggagt agaaattata
    63421 aaatcaactg gatgtgattg tgttttaggt cttggtggtg gaagcattat ggatgctgcc
    63481 aagagtatag ctttttctta taaaaaccca ggagatttaa atgattatat atttggaatt
    63541 agacaaggtg aagaagcttt acctataatc ttagttccaa caacatcagg tacaggtagt
    63601 gaaggaaatt gttttgcagt acttacaaat tcagaaacta aagataaaaa atctataaag
    63661 aagaatagta tgtatgcaaa agcatcaata attgacccag aacttatggt tacaatgcca
    63721 aaacatataa ttgcatcagt tggatttgat gcacttgccc ataatatgga agcatatctt
    63781 tctaatggaa gaaaccctct tgcagatgta caagctattt atgggattga acttatatca
    63841 gaaaacctaa taaaggtata taatgatgtt aatgatttag aagcatggga aaaaataaca
    63901 cttgcaagta ctattggagg aatgacgatt ggtacagcgg gtacagcctt gccacatgga
    63961 atggagcatc ctgtaagtgg tcttagagat gtaatgcatg gaaaaggact tgcagctttg
    64021 actcctacta ttatagaaag ttcatgggat agtgatattg aaagatttgg taatatttca
    64081 aaggtattag gaggaacttg tgcagaagat tgttcagatg caattagaaa tttccttaaa
    64141 aaaatagatt tagatataaa actaagtgat ttaggtattg aaaaatcaga tatagattgg
    64201 ttaactgata attgtatgaa aatatcagtt gcaaatatta agagacatcc aaagtatttt
    64261 aataaggaac aaataaagga aatgtaccat aagtctttat aat
//
LOCUS       DAGSRM010000012        60228 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.012, whole genome shotgun sequence.
ACCESSION   DAGSRM010000012 DAGSRM010000000
VERSION     DAGSRM010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 60228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60228
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.012"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            67..726
                     /gene="cprR"
                     /locus_tag="MJE33_000859"
     CDS             67..726
                     /gene="cprR"
                     /locus_tag="MJE33_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CprR"
                     /protein_id="HBY9646827.1"
                     /translation="MSKILVVDDELDMLKLIENVLKRDGHEVKVISDVEKAMNMDFNY
                     FNLIILDVMMPKMDGFEVCKTIRNKVDCPILFLTAKNMESDIMYGLGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVSIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTITEHIKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
     gene            937..1995
                     /locus_tag="MJE33_000860"
     CDS             937..1995
                     /locus_tag="MJE33_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBY9646828.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPKKIKYFMRITIKHLSKIKFLKFLDNKIESIESFID
                     DYSKAINFFAKNKKVLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILTLQAFL
                     YMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSGIFTVI
                     THYRMKNRIGRNIVLSEN"
     gene            2246..4390
                     /gene="fdhF"
                     /locus_tag="MJE33_000861"
     CDS             2246..4390
                     /gene="fdhF"
                     /locus_tag="MJE33_000861"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2660..2662,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HBY9646829.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKDNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYTASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKAWGVKLSPNNGYSLTQVPDLVLKEK
                     KLKAYYIFGEDPVQSDPDASEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIETQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAEKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYQSLKDKMTATNI"
     gene            4439..5272
                     /gene="fdhD"
                     /locus_tag="MJE33_000862"
     CDS             4439..5272
                     /gene="fdhD"
                     /locus_tag="MJE33_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HBY9646830.1"
                     /translation="MNNIYSKTKLQENSKDKVFENSYSLVDYKYLNSFNVEVSKINEH
                     EVSIDNEEIIAEYPLSFILNDKYVNTFLCTPYNLEELIVGFLKTKGYIENKKSLLKFE
                     INESERFAKVTIQEKQKNQDEQIIFLNSLDYITCAPVKSNIKINYDTIYSIMDKNLNS
                     SELFKNTGGVHSVSVFNFDKKIVTCEDVARHNAMDKAIGFCVLNDITLKDKIVAVSGR
                     ISLEMILKAAMTQIPIVVSKSAPTNLSIELSKKLNITLIGFVRGRRMNIYANPQRVIK
                     K"
     gene            5283..5456
                     /locus_tag="MJE33_000863"
     CDS             5283..5456
                     /locus_tag="MJE33_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646831.1"
                     /translation="MCTKKEKQRYSKCEICGVLIDDTDKVYNNTYNLCKKCKRNISTE
                     KIVKYINIYDESK"
     gene            5514..6065
                     /locus_tag="MJE33_000864"
     CDS             5514..6065
                     /locus_tag="MJE33_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBY9646832.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRHCEDAPCLNSCPQKAIVKENNIMSVNEEKCIGCKTC
                     LLACPFGAIDLLPQYEDGKEVEQVILDENKKIAYKCDLCKDNEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            6090..7466
                     /locus_tag="MJE33_000865"
     CDS             6090..7466
                     /locus_tag="MJE33_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBY9646833.1"
                     /translation="MIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCVMCG
                     QCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYNVCDIDKVKKALSDPNV
                     FTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIMEEGS
                     ELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYGAEI
                     NNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGIDFKN
                     LREEKFDSPLGSYSGAGTIFGVTGGVMEAAIRTGYELITGESIPDVEVKQVRGENGFR
                     KSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQPKIL
                     LEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLHTTY
                     CIGKEVAK"
     gene            7466..7981
                     /locus_tag="MJE33_000866"
     CDS             7466..7981
                     /locus_tag="MJE33_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBY9646834.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNPDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(8059..9342)
                     /locus_tag="MJE33_000867"
     CDS             complement(8059..9342)
                     /locus_tag="MJE33_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugated bile salt MFS transporter"
                     /protein_id="HBY9646835.1"
                     /translation="MNTLLNNKFSIKNFSNAWVIVLGCMLIQAVPFSIVASIQPQFIS
                     YVVEGEGFSLASFSLIFTIGTIASAIASPFIGVLFSKVNIKILYLLGCIISSIAFATF
                     SICTELWQFYTVSCIVQIGATIISAIGIPVLISAWFDEESKGKALGFAFAGGSIGSIF
                     LQPLAVRLLATQGYSRSYLIFGIIALIVGIPVILFILRMPKDINEKVKSKGKKEKASD
                     TISPSVWGYTLKEAKKIKYFWIMTIGFIFVGIYVSSLSVQYPTYLRNYLNPILIGTVG
                     STFALFALIGNLFGGLLFDKFGQTKPLIISFVLVLIATLSLLLVDKSSTFAFVFAILK
                     GLSVFAYMIGPAYLTGSFFGQKEFGSIYGIVQLIFAVGFALGSTLFGLLVDNFGYRFS
                     WSIIILSVIIAYTLLIITSIGMKNLNKERLDKLMQ"
     gene            9530..9952
                     /locus_tag="MJE33_000868"
     CDS             9530..9952
                     /locus_tag="MJE33_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HBY9646836.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMLVNFNKKELSLIKNVSNFAGIRD
                     HIILSDKNGENIIRDILDGNISNECENGIKSKSVIFNNTPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
     gene            10054..10992
                     /locus_tag="MJE33_000869"
     CDS             10054..10992
                     /locus_tag="MJE33_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBY9646837.1"
                     /translation="MKVLFTINYGKEKFDKIRELGYDVIHYSEDSIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKEEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGVE
                     IWGVNTDGSSKKYFDKCFATEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECVEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYIMENKPVDNVVDISRGY"
     gene            11178..13202
                     /gene="ligA"
                     /locus_tag="MJE33_000870"
     CDS             11178..13202
                     /gene="ligA"
                     /locus_tag="MJE33_000870"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBY9646838.1"
                     /translation="MSVKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKRIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGVVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEEYDLESHSESLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLDYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKIVDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     IDFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVDLERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDEVISATSEQLEELEEFGSIMANSVVEFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKDMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLTKANDLGIKVIDEERFLELLKLSSKDEVKAVLES"
     gene            13362..14711
                     /gene="rph"
                     /locus_tag="MJE33_000871"
     CDS             13362..14711
                     /gene="rph"
                     /locus_tag="MJE33_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBY9646839.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRAIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFVAVAEAIYKLYKAKTIKEMPITNFV
                     SAISVGIVNGIHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRADLNLL
                     LELGEKGNKELIRLQREALGEISDEILGMDYGNEVVIATNNAHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIEIVEDGKTFEHNALIKARTIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNIPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            14711..15181
                     /locus_tag="MJE33_000872"
     CDS             14711..15181
                     /locus_tag="MJE33_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBY9646840.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTNMLEKKATEVDAD
                     IVIFGHTHTPFREIKDGVLYINPGSTSLPRGVSYKSFVIMDIEEDDIKIEEIRI"
     gene            15375..16661
                     /locus_tag="MJE33_000873"
     CDS             15375..16661
                     /locus_tag="MJE33_000873"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBY9646841.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLVGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRAKLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            16811..17395
                     /gene="clpP"
                     /locus_tag="MJE33_000874"
     CDS             16811..17395
                     /gene="clpP"
                     /locus_tag="MJE33_000874"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBY9646842.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEILSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            17414..18664
                     /gene="clpX"
                     /locus_tag="MJE33_000875"
     CDS             17414..18664
                     /gene="clpX"
                     /locus_tag="MJE33_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBY9646843.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQAADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(18735..19262)
                     /locus_tag="MJE33_000876"
     CDS             complement(18735..19262)
                     /locus_tag="MJE33_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBY9646844.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATIGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(19250..19762)
                     /locus_tag="MJE33_000877"
     CDS             complement(19250..19762)
                     /locus_tag="MJE33_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBY9646845.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            19942..22305
                     /gene="lon"
                     /locus_tag="MJE33_000878"
     CDS             19942..22305
                     /gene="lon"
                     /locus_tag="MJE33_000878"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HBY9646846.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGRVK
                     KIEQEDGYFRAVIEEIVFDSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLGNIPRPLLDRMEVIEVSGYIEEEKLNI
                     AKKYLLPKQIKEHALKENFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVINKSDLETYLGKDMFKYQLAEVNPQIGLVNGLAWTEVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDPEFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            22295..22906
                     /locus_tag="MJE33_000879"
     CDS             22295..22906
                     /locus_tag="MJE33_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBY9646847.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(23171..24541)
                     /locus_tag="MJE33_000880"
     CDS             complement(23171..24541)
                     /locus_tag="MJE33_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY9646848.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYTLGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPANEKGKAIGITGAVVGIGNMSGPVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQNKSFDIPGLLLFASCTTLILLAMNEKGNT
                     RLYLGITALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNIIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGYVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATIRNL
                     SFALGTAFFSSFFAQSLTYNQKFKSHTSAYVIASNQSYWIAASVCFIGLILTVFFMRK
                     TDKSIS"
     regulatory      complement(24716..24831)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            25119..26198
                     /locus_tag="MJE33_000881"
     CDS             25119..26198
                     /locus_tag="MJE33_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBY9646849.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFCLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNKQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVKKVY
                     YLDGEIGTGKTTLLNKVYKKAIEKGLNVEVYHYPLIPEKIETVLLTDLDIGITISTTF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDKLIKRILKYEENL"
     gene            26590..27270
                     /locus_tag="MJE33_000882"
     CDS             26590..27270
                     /locus_tag="MJE33_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBY9646850.1"
                     /translation="MKKQYVFIILTSTILGILVGTIFRDYSNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKDKLDKEINSMKQNQKNSEEISEIETLRDSLSYSDVKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKVNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            27263..28642
                     /locus_tag="MJE33_000883"
     CDS             27263..28642
                     /locus_tag="MJE33_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBY9646851.1"
                     /translation="MIKIILEESSLPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDI
                     YALQDLRLATGKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENA
                     SDIHIEPFKEHLIVRMRVDGELKEVSKFLVDVYPSLSTVIKLKASMDITEKRLPQDGR
                     VDIKLGGNLIDIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSEEAIKSINRIINK
                     RAGILLVTGPTGSGKTTTVYSLLNDLKGINKNIMTIENPVEYKMDGISQIQVNSKVGL
                     SFDVGLRAILRQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLL
                     EMQIPPYLLNASLIGVISQKLVRKVCNHCSHEIMIKDNFSGDVNTKVAVGCEKCNDKG
                     YFGRTAIYEILEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLE
                     ECLVVNEMI"
     gene            28813..29898
                     /locus_tag="MJE33_000884"
     CDS             28813..29898
                     /locus_tag="MJE33_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBY9646852.1"
                     /translation="MRLSKNIFYNLEKFYLVLNRISDKEIRVICKEIGILLESGCEIT
                     KIFEIIESQSSKKAKNLLSIVSNHIQKGNSIAESFQITGIFSKFFISMIKAGETSGNL
                     DIIMSDLSNYYDKEYKLKMKIITISIYPIILIILSILSMLFIFVFVIPNFQVVFTNNG
                     IEPPLITRVLMGISTVVTNNLAYIIFSFILFSIGSIYFFITNDNIKKLINSLKFKIPF
                     IKKINQLVATTRFSRALSVLISSGIQIVEAIDMSASVIDNEFIYERLKISNNYIKKGN
                     SIGKSLNLANVFPKLFISMANIGEESGKLDKSLNVINKFYENELNNKIEQIMKFIEPM
                     LIVFMGLIIGTFILAMVVPMFDAITNF"
     gene            29952..30311
                     /locus_tag="MJE33_000885"
     CDS             29952..30311
                     /locus_tag="MJE33_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBY9646853.1"
                     /translation="MINLINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKL
                     NADYTNAANIVTAASMAINDDEKTIDSLSVETLKEKGYLNTVPVPQSTSGKFELVIND
                     SGTDISVNINSKQFYPK"
     gene            30364..31236
                     /locus_tag="MJE33_000886"
     CDS             30364..31236
                     /locus_tag="MJE33_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HBY9646854.1"
                     /translation="MAIIKKILNLEINDDYIRLVFLRKGHKKVFIDKSIIKEINFDIK
                     NDSNSIEKIKYIEDVVRFVKEEIGKSKILYKNLYFNIQIQDIVIRNVEVLNTKKKRDI
                     LSMIEFEITQYIPININNYSIKYKVINSKNDKLNIQVILFPKSIIQLIKNISENLNMN
                     PKTININFDILQKLINLNLIENFSEEGVFIECKKSQLIINITKDKKIRETYLLSKEGK
                     SYEFIKRLLRDFKYVYYYGIENCFIEEYFKYTDKSIEVNPLRLKDKVKVENNFDADLE
                     DNRINYISNIGMII"
     gene            31237..31680
                     /locus_tag="MJE33_000887"
     CDS             31237..31680
                     /locus_tag="MJE33_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646855.1"
                     /translation="MKVDKTINFLNGFKKTKPCNKIYKIIIVLILTCILFKSFMYVKS
                     INELEDYISQNKLVDKSKNTLPTTDKTREKFSKIKLNEIKKIYETIGINSITKISIDK
                     NKLEMEGVCSDLDVLNNLRKIKYIKYYYIDSIVRDGEYYRFRIKS"
     gene            31695..32279
                     /locus_tag="MJE33_000888"
     CDS             31695..32279
                     /locus_tag="MJE33_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646856.1"
                     /translation="MRLNNREKNLLKILLILVIILASDIAVSYLEENLTSLKNEKQNI
                     INKINNQDNYQIKDNKHIDDKYIESVESNKNEELKNNFNNSFLDKKTNKDIVLLVENE
                     LRDIVNVEYINQESSLDNHNKKQVNLEIKVNGELANVLKLEGVMKKLRLNNKLEKIEI
                     NKLKVNNFDVQNIPNEYKQEKDNIVECKMTVRVI"
     gene            32532..33569
                     /locus_tag="MJE33_000889"
     CDS             32532..33569
                     /locus_tag="MJE33_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBY9646857.1"
                     /translation="MLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQYTDL
                     YSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKKVMTV
                     GFLEENGTNLSQSEVSILKSMFESYVSIYEVKEVSAGKIVLKDCLSGNELCTEDVKLL
                     RSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKDMKSF
                     LIYHTHILYKYIQQLLDPSIAEYLKRERDKNDTKVNSENELAEEECKVLDTLKENVEK
                     ENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDISSST
                     LGKRYKDLKIS"
     gene            33650..34015
                     /locus_tag="MJE33_000890"
     CDS             33650..34015
                     /locus_tag="MJE33_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646858.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINIAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            34020..34337
                     /locus_tag="MJE33_000891"
     CDS             34020..34337
                     /locus_tag="MJE33_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646859.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEITLGSEKLYVCELRVVLI
                     KVNSLYVERLHKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            34385..35989
                     /locus_tag="MJE33_000892"
     CDS             34385..35989
                     /locus_tag="MJE33_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646860.1"
                     /translation="MLRVSNVKLGIDKDISLLKDVILKKLKIKEKNLVKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILNSKIKDVTKIKEIRYVNVPVGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDIVVSNDSIQSVIINDTEKIDTDTIILAVGHSARDTYEMLYSRGIKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASASNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNSLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGSVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSIKNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            36113..37741
                     /locus_tag="MJE33_000893"
     CDS             36113..37741
                     /locus_tag="MJE33_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBY9646861.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPVALGVGIILYLFGNKPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPLLGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPVVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTNLIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIDKTYRRERLINDLQKAILNYLL
                     KLSKAPLDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNISFSEEGQSELD
                     VMYNRVVSAYTYALESMRTDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            38006..39355
                     /locus_tag="MJE33_000894"
     CDS             38006..39355
                     /locus_tag="MJE33_000894"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBY9646862.1"
                     /translation="MGKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSAEKIKSDSDVLLVIGIGGSYLGSRAAIDMVSHSFRNG
                     LKKEQRKAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDASKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNPDLKSNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYVSEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITISSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            39511..40590
                     /locus_tag="MJE33_000895"
     CDS             39511..40590
                     /locus_tag="MJE33_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBY9646863.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVSKSTGSSTP
                     IVVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTSNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTNMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASQAEGLGFAIPINTAKSIVEEVIKNGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TFSKGASTKITVNRGGKAVTINVNF"
     gene            40935..41843
                     /locus_tag="MJE33_000896"
     CDS             40935..41843
                     /locus_tag="MJE33_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBY9646864.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSKCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIEKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKCADLFLYDIKLIDEDKHIKFTGKSNDLILKNVKILSELGANIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKRLFEASNFNTKIGG"
     gene            41848..44217
                     /locus_tag="MJE33_000897"
     CDS             41848..44217
                     /locus_tag="MJE33_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HBY9646865.1"
                     /translation="MARGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLKELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKKDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRNFQSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDANFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRPTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPQDYKDLIVRVAGYSDHFNNLSRTLQDEIIGRTEQTF"
     gene            44542..45345
                     /gene="proC"
                     /locus_tag="MJE33_000898"
     CDS             44542..45345
                     /gene="proC"
                     /locus_tag="MJE33_000898"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBY9646866.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSELVKAEDITVSDLSQVALDKV
                     KEEFGVNTTTDSKVAVTNSDVVIVALKPNICEKALTPLKELIDANKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKALFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
     gene            45943..48699
                     /locus_tag="MJE33_000899"
     CDS             45943..48699
                     /locus_tag="MJE33_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY9646867.1"
                     /translation="MRDEILSFVNECVLFNNFDELKKESGIITEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKNKINDVVGPTKKSIYENLDELKNECKLLKQETV
                     FNSIIGYENSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSEFLQTFLRRIPINI
                     YVPNIEERGTIEKRKIVEHFIYEESKVLGLPIEITQITLNAILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIIFSPKQFSIEM
                     SNGRNKRNIIYQSFIKCLDYFDRLEKNEITKEKFSKKSSNLMIRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLSDEYKALENKL
                     LIYLKKESYKEYSIISKISTLITNRLDMYLNNVDTIIMIIFIKSTIIRMKFNQVNATI
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIFEDLKNKINSPMLFIDQISTISALEVGNLLMQGKNIEEIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGTAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVMAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSRVVENEKIKNIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLSNDKKISLYVHLSCM
                     IERLVRQTEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQIPISEIGYIYNIIY
                     GLPQ"
     gene            48831..49310
                     /locus_tag="MJE33_000900"
     CDS             48831..49310
                     /locus_tag="MJE33_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY9646868.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTKDALVVAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDDDDINNFKQIGDLGIDIEFRMLPRDKKKQLSDLIK"
     gene            49326..49748
                     /locus_tag="MJE33_000901"
     CDS             49326..49748
                     /locus_tag="MJE33_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBY9646869.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLTNIEYFCMTTDKSADDA
                     EKEIRSILSNNKDSEEFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSILL
                     LVEEDTKLIVKSGLEEAKNGIVYVNELLENQNKIKNVM"
     gene            49788..50579
                     /locus_tag="MJE33_000902"
     CDS             49788..50579
                     /locus_tag="MJE33_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBY9646870.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMLDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGIAGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQKAVFYAKQADFKKVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTALINAIPKVILDGLGVAGTLLPAVGFALLMDMLLSKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAINTLTEGEIDFE"
     gene            50572..51372
                     /locus_tag="MJE33_000903"
     CDS             50572..51372
                     /locus_tag="MJE33_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBY9646871.1"
                     /translation="MNKLTKKELRSMFWRSFVLQGAFNYERMQNLGYCYSMLPAIKKL
                     YNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTALM
                     GPLAGIGDSLFWGTFRIIAAGVGVSLAKEGNIFGPLLFLLLYNIPAFALRIFGLKYGY
                     QVGVNSLERIQREGLMEKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVILQD
                     ILDKIIPNMLPLTFAFVIYYMLKRKVSVTKLTIGTIVTGIALHAIGLL"
     gene            51420..52475
                     /locus_tag="MJE33_000904"
     CDS             51420..52475
                     /locus_tag="MJE33_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBY9646872.1"
                     /translation="MTIQDYMLETPVRMREIISNADSLFNEVKRTNLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGIISSEKYNEEINKILDAINRFEAVYKLSKQWIERNKEKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGVDPSV
                     PKDTRFHMKLGSKKLNK"
     gene            complement(52654..54600)
                     /gene="feoB"
                     /locus_tag="MJE33_000905"
     CDS             complement(52654..54600)
                     /gene="feoB"
                     /locus_tag="MJE33_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBY9646873.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLSEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILSRCTKTTYTQKKSISDKIDNIVLNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSDMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFTSFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIIEALIGGIIIVLALFILFNKFKSTKNGNVCSGCS
                     ICSGCPSANSCSSKEDSNKKNNAH"
     gene            complement(54618..54836)
                     /locus_tag="MJE33_000906"
     CDS             complement(54618..54836)
                     /locus_tag="MJE33_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBY9646874.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            55042..55551
                     /locus_tag="MJE33_000907"
     CDS             55042..55551
                     /locus_tag="MJE33_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBY9646875.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKDL
                     SLIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            55629..55790
                     /locus_tag="MJE33_000908"
     CDS             55629..55790
                     /locus_tag="MJE33_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HBY9646876.1"
                     /translation="MANNELEALKNEIEAVREEINTYIEYPEIFQEELVESSKKIDIL
                     INKYMYLSK"
     gene            55941..56606
                     /locus_tag="MJE33_000909"
     CDS             55941..56606
                     /locus_tag="MJE33_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBY9646877.1"
                     /translation="MIDIGSKEIAIRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGIAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKNQLIVRNMEIECRNEEYWKVNFFVSYAKRIHLHDVMKEINMIEGVINVD
                     YLD"
     gene            56672..58456
                     /gene="pepF"
                     /locus_tag="MJE33_000910"
     CDS             56672..58456
                     /gene="pepF"
                     /locus_tag="MJE33_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBY9646878.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSSKEEIEKDISKVKNLIQEVKEYK
                     GKLSESKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDEKLSFYTKYIKDILRDKPHTLSEREEEILAATS
                     ELSTIPENVYDMLAYADMEFPEIEDEEGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEEYL
                     KVIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLIHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVHRQTMFAEFEKIVHQRVESGEPLTADEFTNIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSTAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            58708..59550
                     /locus_tag="MJE33_000911"
     CDS             58708..59550
                     /locus_tag="MJE33_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBY9646879.1"
                     /translation="MFKKKIGVLLASTMLVGALAVGCSSNSGGSGDSGDSSKVSVSGS
                     TSIGPLMETIGEKFQEKNQGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEEKSA
                     GLKETKIAIDGIALITNTANSVKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVSREE
                     GSGTRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKVNSL
                     KINDVEATDANVIANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQALVSEKGFI
                     KVNQ"
     regulatory      59783..59866
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
BASE COUNT        24430 a         6482 c        10756 g        18560 t
ORIGIN      
        1 aattttaaag atattattaa atatataaat tagaatgcta ttagatggat aaatggagga
       61 gttattatgt ctaagatatt agtagtagat gatgagttag atatgttaaa gttgattgaa
      121 aatgtgttga aaagagatgg acatgaagta aaggtaatta gtgatgtaga aaaggctatg
      181 aacatggatt ttaattattt taatctaata atattagatg ttatgatgcc taagatggat
      241 ggttttgaag tatgtaaaac tataagaaat aaggttgatt gtccaatact gtttttgaca
      301 gcaaaaaata tggaaagtga tattatgtac ggtcttggta taggtgcaga tgactatata
      361 accaaacctt ttggtgtagg agaattaagg gctaggataa ctgctcattt aaggcgagaa
      421 agtagagaaa aaaagaattt attaactatc tcaaatgttc aatttaactt tttaggcaaa
      481 gaagtttcaa ttgatggtaa aaaaattaat tttactaaaa gtgaatatct aatatgtgaa
      541 tttctagcaa aaaataaggg acaagtattt tcaaaagaaa gaatatatga gtcggtatat
      601 ggttttgatg gagaaagtga tataagtact atcacagagc atataaaaaa tatacgaagc
      661 aaattaaaac aatatgatat ttatacaata gaaactgtat gggggatagg gtataaatgg
      721 gtgtaataag tataagtgtt gtttagtgaa aatattatat atatctacaa aatgtttgta
      781 aaatattaat ctatatatta aaatttaatt tatagattaa tattttttgt aaatgacaaa
      841 attgtaaggt attttgttat aaaatattaa gattcatagt gtatactaat aatatagtat
      901 ttagtataaa tcttgagcaa aggatggtta tttctaatgg atagactaaa gaatattaga
      961 aagagtttac tacagtatac atttttattt ttgttaatag gtataacaat ttacctagtg
     1021 tttaaaacat tggatattaa aatgctatca aatgttatgg ttatggtaga taagaagttt
     1081 ttattcatag gtgctttggc aataatgtgt cacataatgc ttgaaggagt agtaatgaag
     1141 ataattatag aaagcactca caaagttaat attagattta ttggttttaa gctagccatt
     1201 atgggatttt attataattt aataactcct tttgcttcag gaagccaacc agtacagata
     1261 tatgtactta aaaaatgtaa aatgccactt agtaaggcaa gtgcagttgt taccaataag
     1321 tctattatct tccaaatagt agttactttt tattgtacaa tacttgtttt aatgaatttt
     1381 gctatgttag aaaaacaaat gaaaatgatt atgcctctag tgtttctagg gatagccatt
     1441 aattcatttg tcttaattat gataatatta gttattttaa atcctaaaaa aataaaatat
     1501 tttatgagaa ttactataaa gcatttatca aaaattaagt ttttaaaatt tttagataat
     1561 aagattgaat ccatagaaag ttttatagat gattatagta aagcaataaa tttttttgca
     1621 aagaataaaa aggtattaat ttcaactata atagtaactt ttattcaatt aagtgcttac
     1681 tttagtgttt cattttggat atataaagca tttaatttac aaggttatag ctatatttat
     1741 atactgacat tacaagcatt tttatatatg gcaatatctc caataccaac acctgggaat
     1801 gtaggagcta atgaattagc ctttttcact atattcaaat cagtattccc tcaacctttg
     1861 atgggatatg cagtattttt atatggtgga tttatgtact atttgattct gattggaagt
     1921 ggaatattta ctgtaattac tcactataga atgaaaaata gaattggcag aaatattgtt
     1981 ttaagtgaaa actaaataag tttaaagaat atatattgag tatattaaaa aatatgtcat
     2041 aagaactaga atattttaat taaatggttg aaatataaag attattatga catatttttt
     2101 ttgaaattta agaggaattg tgagaaaatt gttgaattta atagatagta ttgcaaaata
     2161 tttatgattt tgcatattat aaaaacactc attatcttta agataaattt tacttatcag
     2221 gggataaatc taagggggga attgtatgga gaaaaaaatt ttaacagttt gtccatactg
     2281 tggagctgga tgccaattat atctagtagt aaaagataat aagatattaa gagctgaacc
     2341 ggctaatggt agaacaaatc aagaaagttt gtgtttaaaa ggtcgttatg gatgggattt
     2401 tctaaatgac cctaaaattc taacacctag gctaaaaaag ccaatgataa gaaaacaagg
     2461 tgtactagaa gaggttgaat gggatgaggc tataaactat acagcttcaa gactaaatga
     2521 aattaaagaa aaatatggac ctgattctat tatgggaaca ggttctgcaa gaggacctgg
     2581 taatgaagca aactatataa tgcaaaaatt tatgagagct acaataggaa ctaataatgt
     2641 tgaccattgt gctcgtgtct gacatgcacc atctgtagcc ggtctggctt actcattagg
     2701 tagtggtgca atgtctaatt ctataccaga gatagaaaat gcagatgttt tatttatatt
     2761 tggatataat ggagcagatt cacatccaat agtggcaaat agaatagtaa aagctaaaaa
     2821 aaatggtgct aaattaattg ttactgaccc tagagtaact gaatctgcaa gaattgctga
     2881 tatacatcta cctataaaag gtggaacaaa catggtttta gtaaatgctt ttggaaatgt
     2941 attaatagaa gaaggtcttt acaataaaga atttgttcaa aatcatacac aaggatttga
     3001 tgagtataaa gaaattgtta aaccatatac agctaaatat gctgaaaaga ttacaggaat
     3061 cccagaagag cttataagaa aagctatgag agaatatgca aaaggtaaaa aagctatgat
     3121 actttatgga atgggtgtct gtcaatttgg tcaagctgtt gatgtagtta agggacttgc
     3181 atcaattgct ctacttacag gtaattttgg tagagaaagc gttggaatag gacctgtacg
     3241 tggacaaaat aatgttcaag gagcttgtga tatgggagct cttcctaatg tatatcctgg
     3301 ctatcaaaat gtaactgatg ataagataag agaaaagttt gaaaaagctt ggggggtaaa
     3361 attatctcct aataatggtt atagtttaac tcaagttcca gacttagtac taaaagagaa
     3421 aaagcttaaa gcttattata tatttggaga agacccagtg caaagtgacc cagatgcgtc
     3481 agaagttaga gaagcattag atgaattgga atttgtcatt gttcaagata tatttatgaa
     3541 taagacagca cttcatgctg atgttatact tccagctact tcttggggag aacatgaggg
     3601 agtatataca tgtgcagata gaggattcca attgatgaga aaagctatag agccacaagg
     3661 agatgtaaaa ccagactggc aaattataag cgaaatttct acagcaatgg gatatcctat
     3721 gaattataaa aatactaagg aaatatggga cgaacttaga caattatgtc caagtttctt
     3781 aggtgctact tatgaaaaga tagaaactca aggatgcgtt caatggcctt gtaagagtga
     3841 aagtatggaa gataaaggaa ctatgtatct gtatgaaggt caaaagtttt ctactcctaa
     3901 tggtaaaggt aatttgttcg ctgctgaatg gagaccccct atggaagttg aagatgatga
     3961 atacccattt agtttatgta ctgtaagaga agttggacac tattcagtaa gaacaatgac
     4021 tggtaattgt agaacactta gttcattaga agatgaacca ggacgtgttc aaataaattc
     4081 taatgatgct gaaaaattag gaattgaaga tgatgaatta gttagaatta gttcaagaag
     4141 aggaagtgta ataacaagag caacagttac tgatagagtt aaggaaggtg caacatatat
     4201 gacttatcag tggtgggttg gagcttgcaa tgaacttaca atagcaaatt tagaccctat
     4261 atctaaaaca ccagagtata aatattgtgc tgttaaactt gaaaaacttg aagaccaaga
     4321 attagcagaa aaatgtgtaa gagaagagta tcaaagtttg aaagacaaaa tgacagctac
     4381 aaatatctaa tgatttattt gatttaagat ttataaaagg cagaaaggct aatattttat
     4441 gaacaatata tactcaaaaa ctaaattaca agaaaatagt aaggataaag tttttgaaaa
     4501 tagttatagt ctagtggact acaaatattt aaattctttt aatgtagaag taagcaaaat
     4561 aaatgaacat gaagtatcaa tagataatga agaaataatt gcagaatatc cacttagttt
     4621 tattttaaat gataaatatg taaatacatt tttatgtacc ccatataatt tagaggaact
     4681 tatagtagga ttcttgaaaa caaaaggata catagaaaac aaaaaaagct tattaaaatt
     4741 tgagataaat gagagtgaac gttttgcaaa agtaactatt caagagaaac aaaaaaatca
     4801 agatgagcaa attatatttt taaattcact agactatata acctgtgctc ctgtaaagag
     4861 caatataaaa ataaattatg atacgattta cagtataatg gataaaaatt taaattcatc
     4921 agaattattt aaaaatactg gtggggtaca tagcgtatct gtatttaatt ttgataaaaa
     4981 aatagtcact tgtgaagatg tagcaaggca taatgcaatg gataaggcta ttggattttg
     5041 tgttttaaat gacataacat taaaagataa aattgtggct gtaagtggaa gaatatcatt
     5101 ggaaatgata ttaaaagcag ctatgactca aattcctatt gtagtatcaa aatcagcacc
     5161 gactaattta tcaatagagc tttctaaaaa attaaatata acgcttattg gatttgtaag
     5221 aggaagacgg atgaatattt atgcaaatcc acagagagtt atcaaaaagt gagaatgaat
     5281 atatgtgtac taaaaaagaa aagcaaagat attcaaagtg tgaaatctgt ggagtattaa
     5341 ttgatgatac tgataaggtg tataataaca catacaattt gtgcaaaaaa tgtaaacgaa
     5401 atataagtac tgagaaaata gtgaaatata taaatatata tgatgaaagt aagtagacat
     5461 atgatgaaaa taactagatg gttggaaata tacaaattga aaggtgggat aatatgaaaa
     5521 ataaaatagg ctcttttgta atagcagacc caaacaaatg tacaggttgt agagcttgtg
     5581 aagtagcttg ttttacaatg cataatcagc ataataatgt gggttataca gttggaacag
     5641 ttgatatacc tgttatacct agactttact tagtaaaagg tgacaatttc tgtatgccaa
     5701 tacagtgtag acattgtgaa gatgctcctt gtttaaactc atgtccacaa aaagctatag
     5761 ttaaagaaaa caatattatg tctgtaaatg aagaaaagtg tataggatgt aaaacttgtc
     5821 tgctagcatg tccttttgga gctatagact tacttccaca atatgaagat ggtaaagaag
     5881 tagaacaagt aattttagat gaaaataaga agatagctta taaatgtgac ttatgtaaag
     5941 acaatgaaaa aatagcttgt ataagtgcat gtcctcagga agcattaaaa cttgttactc
     6001 caatagatga caaaaaagct aagaatagac aagctgcact tagtttattg ttaacaaata
     6061 aataaaggat acaggggtag gtgacgttta tgataataaa tatagataag gatttatgta
     6121 ctggatgtag agaatgttca aaggtctgtc cagtaaatgc tattgaaggt gaagagggaa
     6181 agccacaaga gataaattta gatagatgcg ttatgtgcgg tcaatgtgtt caaacttgta
     6241 aatcttatgc ctcagttata gatgaaggat ttgagttttt acaagagaaa aaacaagaaa
     6301 gagagatacc agagtctata aatgaaccta tatttgcagc atacaatgta tgtgatatag
     6361 ataaggtgaa gaaagcttta agtgacccaa atgtattcac tatggtccaa tgtgcaccag
     6421 cagttagagt tgctttagga gaagattttg gatattattt aggatactta ggtgctggga
     6481 aaatggcagc agcacttaga gctttaaatt ttgatagagt ctacgatact aattttgcag
     6541 cagatttaac tataatggaa gaaggtagtg aattaattaa aagagtgaca gaaggtggaa
     6601 ctcttccaat gtttacatct tgttgtccag cttgggtaaa attcatggaa aaaaattatc
     6661 ctaaattaac taatcattta tcttcttgta aatcacctca gcagatgggt ggtgcaatat
     6721 ttaaaactta tggtgcagag ataaataatg tagatgcatc aaagatattt agtgtttcta
     6781 taatgccatg tacatgtaaa aaatatgaat gtgatagaga agaaatggat tctagtggat
     6841 atagagatgt agatgtagta ttaactacta gagaactagc ttatttaata aaagacatgg
     6901 gaattgattt taaaaattta agagaagaaa aatttgatag tcctttaggt agttatagtg
     6961 gagctggaac tatctttggt gttactggtg gggttatgga agctgctata agaacaggat
     7021 atgagcttat aactggagaa agtattcctg atgtagaagt aaaacaagta agaggagaaa
     7081 atggttttag aaagtcaact attaaaatag gagacttaga cttaagagtt ggagtagtaa
     7141 gtggattaaa aaatgtaata ccagtgcttg aagacttgga aaaaggaaaa ttagatgtag
     7201 attttattga agttatgact tgtcctgttg ggtgtgtaag tggtggaggt caaccaaaaa
     7261 ttttactaga agaatataag gaattggctt atgaaaatag aacaaaagca acatacgtgc
     7321 atgatgaaca tttagcaatt agaaaatctc atgagaatcc tgatattaaa aaactgtatg
     7381 atgaatatct ggtagaacca ttaggagaaa aatctcatca tctattacac actacatatt
     7441 gtataggaaa ggaagtggct aagtgatgaa ctattttgtt atagcagacc ctgataaatg
     7501 cataggatgt agaacttgta tgataggatg tgtagttgca cattcagatg aagatatttt
     7561 ttaccaaaat ccagatgaaa taaattttaa tccaaaactt tctgtcataa agacaaaaga
     7621 agtaagtgct ccaatacagt gtagacattg tgaagatgct ccttgtgcaa aggcatgtcc
     7681 aaatggtggt atagttaggg ttggtaacac aataaaaatc aatgaagaaa attgtatagg
     7741 atgtaagact tgtatgttag catgtcctat aggtgcaata gacatagtta cattaaaaga
     7801 tgtagatgaa ggaaagcttt gctttagaga aagaatgaca gcaaataagt gtgatttttg
     7861 tatagaatcg ccagaagggc ctgcatgtgt aaatgtttgt ccaactaagg catttactat
     7921 agttaaggat gaggatatag atagtaaagt taaaaataag agaaaattag ctatattgta
     7981 atataaaaaa gatggagttt tatttatgat taaaagctcc atctttttta ttataatatt
     8041 tatatatcta aaaatcaatt attgcattag tttatctaat ctttctttat tcaaattttt
     8101 cattcctata gaagttataa taagtaaagt ataggcaatt ataactgata aaattataat
     8161 agaccaagaa aatctgtatc caaaattatc aactaacaat ccaaataatg ttgaacctaa
     8221 tgcaaatccc actgcaaaaa ttaattgtac gattccatat atgctaccaa actccttttg
     8281 accaaagaaa gagccagtta aataagcagg accaatcata taagcaaata cagaaagacc
     8341 tttcaaaatg gcaaatacaa atgcaaaagt agaagattta tctaccaata gtaatgataa
     8401 agttgctata agaaccaata caaaagatat aataagtggc tttgtttgac cgaatttatc
     8461 aaacaataat cctccaaata aattaccaat gagagcaaat aaagcaaatg tggagcctac
     8521 tgttcctatt agaataggat ttagataatt tctaagataa gttggatatt gtacagataa
     8581 tgatgaaaca tatataccaa caaatataaa tccaatagtc ataatccaaa aatatttaat
     8641 ttttttagct tcttttaatg tataacccca tactgaagga gaaattgtat cagatgcttt
     8701 ctctttttta cctttagatt ttaccttttc attaatatct ttaggcatcc tgagtataaa
     8761 taatattaca ggtataccaa ctattaatgc tataattcca aatattagat aagaacgact
     8821 gtatccttga gttgctaata agcgaactgc aagtggttgt agaaatatac taccaataga
     8881 accaccagca aaagcaaagc ccaaagcttt tcctttgctt tcttcatcaa accaagcact
     8941 tattaaaaca ggtattccta tagcagatat aattgtagcg ccaatttgaa caatacatga
     9001 tacagtgtaa aattgccata gttcagtaca tatagaaaaa gtagcaaaag caatactaga
     9061 aattatacaa cctaataaat atagtatctt aatattgact ttagaaaata atacacctat
     9121 aaaaggtgat gcaattgctg aggcaatagt accaatagtg aatattagag aaaaagaagc
     9181 taaactaaag ccttctcctt ctacaacata acttataaat tgaggctgta tacttgctac
     9241 aatactaaat ggaacagctt gtatcaacat acaaccaagt actataaccc aagcatttga
     9301 aaagttttta atgctaaatt tattgtttag taatgtattc atggtatctc tcctttaggt
     9361 tatgatttgt agatattaat ctaattattc taattttata tattagtttt ggatagccta
     9421 actatttaaa tataatttgg gtattaatat aaaaattaac tataaaaatt gattatttta
     9481 aatgtatggg gtaaattaaa gtatataaat aaatgaaagg atggataaaa tgtcatttga
     9541 aaaaataaat aataataacc tagataaaat acacaacagg aagtgtatta tgcttgtaaa
     9601 ctttaataaa aaagaattat cattaattaa aaacgtaagt aactttgctg gaataagaga
     9661 ccatatcatc ttaagtgata aaaatggaga gaatataata agagacattt tagatggaaa
     9721 tatatcaaat gaatgtgaaa atgggattaa gagtaagtct gtaatattta ataacactcc
     9781 aagcacacaa gtaaatgcat ttttagaagg gctaaaaaaa gtgagaataa atagaccttt
     9841 tacagctatg gtaacagaga catctattga ttggacattg aataatttaa tagaaaattt
     9901 agttcaagaa cgtaatgcta tgtctttagg aaaaacaatt gaacataagt aaatttaaga
     9961 tatcttatag taattttaac taatgtacat tgagtatcat attatatttt agaatataaa
    10021 attttaaatg tgggtagatg gaggagattt gagatgaaag ttttatttac aataaattat
    10081 ggaaaagaaa aatttgataa aataagagaa ttaggctatg atgtcataca ttactctgaa
    10141 gatagtattg aaaataatga ggatgtaaat acagcagatg tactagttac atacaatcca
    10201 tttgagagat tagatatttc taagatgaaa aatttacaat atatacagac aactagtgta
    10261 ggtatagacc aacttccaaa agaagaggta ttgaagagaa atattaagat agctaataat
    10321 agaggtggat atagtgtacc tattggtgaa agtattgtat tgtatatttt agaaatatat
    10381 aaaaatagtg caaaattttt taaacaacaa caaaataaag aatggaagat ggatttttct
    10441 gtatcagaat tgtatggaca aagagttgga tttttaggaa caggtactat atcttcagaa
    10501 gcagcaaaaa gattaaaagc ttttggtgta gaaatatggg gtgtcaatac tgatggaagt
    10561 agtaaaaaat actttgataa gtgttttgct actgaggata tgaatattgt atttgaagaa
    10621 tgtgatatag tagttgtagc aatgccttct acaaaaagta cagatggaat agtaaacaaa
    10681 gatatgttta aacttatgaa agatggttct gtatttataa atgtaggtag aggaaataca
    10741 gttaatcaga aagatttaga agaatgtgta gagaaattta gaggggtagc attagacgta
    10801 tttgaaagtg aacctttaaa caaggacaat aagttgtggg agtgcgaaaa tgtcatagta
    10861 actcctcata attcttgggt gtcagataaa aacaaagaga gaacatttaa tatggtttat
    10921 aataatttga aacactatat aatggagaat aaacctgtag ataatgttgt agacatatca
    10981 agaggctatt aaaaataaaa aattttgata aaaataaaaa ctcttattag tcgtattaaa
    11041 gatactaata agagtttttt ttgtaataaa tttaaagcca aaaaagtgat atactaaaaa
    11101 atatataaat aggagataat taattatagg gaaatttcaa aataatttaa ttttaaaaat
    11161 atgaagaggt gcaaaaagtg agcgtaaaag atgaaattaa taaacttata gagacaataa
    11221 actaccataa tgaaaggtat tataatcaag atagcccaga aatatcagat tatgaatacg
    11281 ataagttaat gaaggagtta atttctttag aagaagaaca tccagaacta aagagaattg
    11341 attctccaac aaatagggta ggaggtaagc cattagataa gttcaatcaa attgtacata
    11401 aaactcctat gttgagttta tcaaatgctt tttcagagca agatttgaga gattttgata
    11461 agagagttca agaatatgtg ggagaaaatg ttgaatatgt tgttgaattt aaaatagatg
    11521 gtctttctgt aggtctaact tataacaatg gagagtttga aaaaggagca actagaggag
    11581 atggagttgt aggagaagat atttctcaaa acttaatgac agtaaaaagc atacctctta
    11641 agataaatga taaaaaagaa ctaatagtta gaggagaagt atatatatca aaagataatt
    11701 ttagaaaagt aaatgaacaa caagaagaac aagaacagcc attatttgct aatccaagga
    11761 atcttgcagc tggttcattg agacaattag acccaaaact aacagcaaag agaccattag
    11821 atatatttgt atttaatatg gaaaatatag aagaatacga tttagaaagt cacagtgaat
    11881 cactagagta cttagaaaaa ctaggatttt cagtaagtca gagttacaaa gtttgtaaaa
    11941 gtatagatga agttattgag catattgaat actggacaga taacaggggt aatttagact
    12001 acgaaataga tggtatggta ataaaagtaa acaatataaa acaaagagac gaaatgggat
    12061 atacagccaa aagtcctaga tgggcaattg catataaatt cccagcagaa aagaagaaaa
    12121 ctaaaatagt agatataata gtagaagttg ggagaactgg tacaataaca ccttcagcaa
    12181 ttcttgaacc agtcagactt gcaggtacta ctgtaagtag agctacactt cacaatgaag
    12241 attatataag agaaaaagat ataaaaataa atgatacagt attagttcaa aaagcagggg
    12301 acataatacc tcaagtttta gaagttataa aagaagaacg tactggagat gaaatagatt
    12361 ttaaaatgcc agaaaagtgt ccagtttgtt cagagccaac aataagatta gaaggagagg
    12421 ctgctgtaaa atgtatcaat atgtcttgcc cagcccaaat aagaagggga attatacact
    12481 ttgtgtcaag ggatgcaatg aatatagatg gattgggaga atctataatt acactgctat
    12541 taaatgaaaa aattattcaa gatgtatctg atttatatta tataaaaaag gaagatgtag
    12601 tcgacctaga gagaatgggc gaaaaatctg ctgacaattt aataaattca attgaaaaat
    12661 caaaagaaaa tgatttatgg agacttataa atggattagg tattaaattt ataggagtta
    12721 aagcagctaa gatacttgca tataacttta aggatttaga tgaagtaatt tctgcaacta
    12781 gtgagcaact agaagaatta gaggaatttg gaagtataat ggcaaacagc gttgtcgagt
    12841 tttttaagga agacaaaaat atgaacgtta taaacaagtt aaaaaatgta ggagtaaata
    12901 caaaatcatt ggataataca ggtgaaaaag tagaaaaaat atttgaaggg atgaaaatcg
    12961 tattgactgg tacacttcct acattaaaaa gaaatgatgc aaaagatatg atagaaaaaa
    13021 gaggtggaaa agctacatct agtgttagta aatcgacaac atttgttttg gcaggtgaag
    13081 aagctggttc taaacttact aaagcaaatg atttaggaat taaagttata gatgaagaga
    13141 gatttttaga attattaaag ttatcatcga aagatgaagt aaaagctgta ttagagagtt
    13201 aaaaagtcgc ttaatttagt agaaaactac tataattaat gatagaatag ttcagtaagc
    13261 taataagatt acgaaaaatg acaataatta atatttgaca aaattaaaaa ttataaaaat
    13321 aaatttataa aaaataaaaa atatgagaaa ggatataaaa aatgaccaga agttatgata
    13381 gaagtaatga acagataaga cctgtaaaga taactagaga ttatacaaag tatgcagaag
    13441 gttctgttct tatagaaatg ggagagacaa aggttatatg cactgcatca gtggaagaaa
    13501 aagttcctcc ttttttaaga aatagtggta ctggatggat aaatgctgaa tattctatgc
    13561 taccaagagc aacacaacaa agaaagataa gagatgcatc taggggaaaa attgatggaa
    13621 gaacacaaga aatacagagg ttaataggaa gagcaataag gtctgttgta gacttgaata
    13681 aattaggaga aagaactatt tgggtagatt gtgatgtgat tcaggcagat ggtggaacta
    13741 gaactgcatc tataacaggg gcatttgtag cagttgcaga agcaatatat aaattatata
    13801 aagcaaagac aataaaagag atgccaataa caaattttgt gtctgcaatt agtgttggta
    13861 ttgtaaatgg aatacacatg ctagatttat gttatgaaga agattctaaa gcacaggtag
    13921 atatgaatat aataatgact gataagtgtg agtttgtaga agtacagggg acaggagaag
    13981 agaaaccttt ttcgagagca gatttaaatt tattgttaga gttaggtgaa aaagggaata
    14041 aagaattaat aaggcttcaa agagaagctt taggtgaaat atcagatgag atattgggta
    14101 tggattatgg taatgaagta gtgattgcta ctaataatgc tcataagcta gaagaaatag
    14161 gtgaaatctt aaaagatttt gaatataaag tgtactcttt aaaagatgtt gatttagcag
    14221 gtatagagat agtagaagat ggaaaaacat ttgagcataa tgcgttgata aaagctagaa
    14281 caatagcaaa aaagactaaa cttatagcaa tatcagatga ttctggctta gaagtagatg
    14341 ctttaggcaa aaaacctggt gtttattcag ctagatatgc aggagaacat gcaacagatg
    14401 aagaaaatag aaaaaaactg ttaaaagcta tgcaaaatat accaatgagc aaaagaaatg
    14461 caagattcgt ctctgcaata gcagtagttt ttcctgatgg aaaagaattt gtagtaagag
    14521 gaatttgtga aggaatgata ggatttgaag aaaaaggtaa aaatggtttt ggatatgata
    14581 gcttatttat agtaaaagga tatgataaga cttttggtga aatacctagt gttataaaaa
    14641 attcaatcag tcatagagca aacgcactaa aacttatgaa acaagagttt ataaaaaggg
    14701 tagtgaagta atgaaaatag gcatagtaag cgacacacat atgatgatta aaaatatgga
    14761 aaaaacaata ccatatttga aagaatgtga cctaataatt catgcaggag ataactttac
    14821 tgattcaaga tatattcata gcatgactaa tgtaggtatt attgcagtta aaggtaattg
    14881 tgactttgat gctgttgagg aagaagttgt ctttgaagtg gcaaataaaa ccatattctt
    14941 atgccatgga gataagtatg gtgtaaaata tggaactaat atgttagaaa aaaaggcaac
    15001 tgaagttgat gctgatatag ttatatttgg ccatacccat accccattta gagaaattaa
    15061 agatggtgta ctttacataa atcctggaag cacttcactt ccaagaggag tatcgtataa
    15121 aagttttgtc attatggata tagaagaaga tgacataaaa attgaagaaa tacgcatata
    15181 aaggtgtatt tatctaaaaa aatggcaaaa aacgctcaaa aaattaaatt ttatcaaata
    15241 agtacaacga tattaatgga acacttgtaa ataaaaaaac ttatgtgata taattaagtg
    15301 gttgaattat aaatagaatg aattataata ccgttatcgg gtattacata aatatcacta
    15361 ggaggattag aaggatgaaa gcagaactaa ttaaaaaaga gggtaataaa gttactttaa
    15421 aaataacagt agataacaat aaatttgaag cagcagtaaa taaggcttat aataaaagca
    15481 gaaataaata caatatacct ggatttagaa aaggtaaagc tccaagaatt gttatagaga
    15541 ctcaatacgg aaaaggtata ttctacaatg atgctataga aatattattc ccagaagttt
    15601 atccagaagc tatcaaggaa ttagacatag acccaataga caatccagat ttagatatag
    15661 aagaaataag taaagacaat gggttggtta tggttcttaa tgtagaagtt aaaccagagt
    15721 ttgaattagg aaactacaag ggtatagaaa tagctaaggt tgagaatact gtaagtgacg
    15781 aaaatgttga tgctaaacta caagaaatgg ttgaaaaaaa tgccagatta gtaagtgtag
    15841 aagacaaagc tttagaagat ggagatacag ctataataga cttcgaaggt tttgaaaatg
    15901 gtgtagcttt tgatggtggt aaaggtgaaa attataactt agttataggt tcaaacacat
    15961 ttataccagg atttgaagaa caattagtag gtaaaaaagc tggtgaagaa gtagaagtta
    16021 atgttacttt cccagaagaa tatcactcac aagatttagc tggaaaacca gttgtattta
    16081 atgtaaaaat aaatgatgta aaagtaaaag aattatctgc acttgatgat gaatttgcta
    16141 aagatactag tgaatttgat tctttagatg aattaaaagc agatgtaaga gctaagttag
    16201 aagaagaagc taaaaataga gcagatgcag aaactagaaa ttcagtagta gaaaaagttg
    16261 ctgaaaatac agaaatagaa ataccagaag ttatgataca acatcaaata gataatatgt
    16321 taaatgaact taactaccaa ttacaatatc aaggatttgg attacaacaa ttactagaga
    16381 tgactggtag aactatggaa gaattaagag aagagagaaa agaagatgct aaaaaattag
    16441 ttaagtcttc tttagtatta gaagcaataa ctaaagctga aggaattgaa gcaacagaag
    16501 aagaatttaa ggctgagtta gaaaaaatgg cttctgctta caatatggaa gtagaaaaaa
    16561 tagaagcttc tttaagagat gctgacaaag aagatataaa aggtcaaata aaaataagaa
    16621 aaacaataga tttattagtt gataatgcaa caatagctta ataaaataaa cgctaactaa
    16681 tatagttgac gttatgaaat gtttagtata taatgatgtg caaatataat ttagttaaat
    16741 gcgtataata atttacagta gcttaaaaaa agctactgta aattatatag aataaagggg
    16801 gtatgaaacc atggcattag tacctgtagt tgttgaacaa acaggaagag gagaaagatc
    16861 ttatgatatt ttttctagac tattaaaaga cagaataatc ttcttaggag atcaagttaa
    16921 cgatgccaca gcagggctta tagtagcaca gctattattt ttagaagctg aagacccaga
    16981 caaagatata catttatata taaattctcc aggaggaagt ataacttctg gaatggctat
    17041 atatgatact atgcaataca tcaaaccaga tgtaagtact atatgtatag gtatggctgc
    17101 ttctatggga gcattcttgt tggcagcagg agcaaaagga aaaagactag cactaccaaa
    17161 tagtgaaata atgatacatc aaccattagg tggagctcaa ggtcaagcaa cagatataga
    17221 aattcatgca aagagaattt taaaaataaa agaaacttta aatgagatat tatctgaaag
    17281 aactggtcag ccattagaaa aaattaaaat ggatactgaa cgtgataact tcatgagtgc
    17341 actagaagcc aaagaatatg gtttaataga tgaagtattt acaaagagac cgtagataga
    17401 gagaggtgtt aatatgtcta aatatgaaga aaaacgacaa ttaaagtgtt cattctgcgg
    17461 taaaaatcaa gatcaagtaa gaagattaat tgcaggtcca aatgtatata tttgtgacga
    17521 gtgtgttgaa ctttgtgacg aaattattca agaagagata gaagatacta tagatgagga
    17581 tactacatct ttaccaaaac caaaggaaat gatggagatt ctaaatgatt atgtaatagg
    17641 tcaagaaaaa gctaaaaaag ctttatcagt tgcggtttat aatcattata agagaatata
    17701 cagtaaaaaa tctagtagta aggatataga aattcaaaag agtaatatac ttttattagg
    17761 acctacaggt tctggaaaga cattacttgc tcaaactcta gcaagaactc taaatgtacc
    17821 ttttgcaatg gcagatgcta catcattgac tgaagctgga tatgttggag aagacgttga
    17881 aaatatactt ttaaaactta ttcaagctgc tgattttgat atagaaaaag ctgaaagagg
    17941 tataatatat atagatgaga tagataagat tgcaagaaaa tctgaaaacc catctataac
    18001 tagagatgtt agtggtgaag gtgttcaaca agcacttcta aaaatattag aaggtactgt
    18061 tgcaaatgtt ccaccacaag gaggaagaaa acatcctcat caagaatttt taaagataga
    18121 tactactaat gtattattta tattaggtgg agcttttgat ggattagaga agataattca
    18181 aaaacgtggt ggagataaaa cactaggttt tggagctaaa atagaaagta aaaaagaatt
    18241 agatttaggg aagctatatg aaaaagtact tccagaggat ttattaaaat atggtataat
    18301 accagagttt ataggaagaa taccagtttt agctacttta gagttgttgg atgaagatgc
    18361 attaatgcag atattacagg aacctaaaaa tgctctagta aaacagtata aaaagttatt
    18421 ggagttagat gatgttgagc tagaatttga agaaggtgct cttcgtgcaa tcgctaaaaa
    18481 ggccatagaa agaaatacag gagctagagg acttagaagt atagttgaaa gcgtaatgat
    18541 ggaaactatg tttgaagtac catctagaga taatataaag aaggttatag taacagagaa
    18601 atctgtgaac gaagactcag taaatcctat catagtactt aaggatcaag aggaaagtgc
    18661 ataagatgat gaaaaggagc tttaaaagct ccttttttta tgttaatatg ctaaatataa
    18721 aataggtatc attattataa aaatgtgagt aatataccta gaataccaaa gacgaataca
    18781 attgcaatgg aaccaatttt tcctttatat aaaagataaa atgatgctat aaatgcaatt
    18841 attccatata aatcccaatt aacttctttg gcagatgaaa aagctgctga taatatgaat
    18901 cctagagtaa ttggtctaag aactgtcaat aagttttcta cctttttatt atttttaaat
    18961 ttagaaaata actttgatat aatagttaga cctattaatg caaaaattgt aacaccaata
    19021 gttgcagcta gagaaccaaa gaagccagca gtcttaaatc ctacaaatgt agcactattt
    19081 attgctacag gacctggtgt agattgtgat atagcaagta aatccaaaaa ctcttgatta
    19141 gttataaagt gatatttaga tataaattct tgctggataa atggtagcat ggcataaccg
    19201 ccaccaaaac taaatgcccc tattttaagg aatattaaga agagtagtat tatttctgac
    19261 atagactata tacacctcct ccaattatta aataaatagg gcttatattg aatacaccaa
    19321 cagataaaac tgctataatt aatagtagta tgttagtttt agactttggt agtttcttga
    19381 acatgctcgt aaaagaatac aatataagag ctattacaac aggcgcaact cctttaaaaa
    19441 atccatctaa aattttagat gatgaatttt taaagtatag atacgataag actaagacta
    19501 tacaaaatga tggtaaagca gagcctaagg tacatataag aactcctggt ataccataaa
    19561 gtttatatcc taataataca gatatattaa ctgccagtac tccaggataa ctttgagcta
    19621 tggataaata atcaataaat tcttcctgct ctaaaagttt ttgtttgttt acaagttcat
    19681 cttgtataag aggaaccata gcgtatccac caccaaatgt aaacgcacca atttttaaaa
    19741 aggttaaaaa caatttcaac ataatagaaa cctccaaaag ataaataaca tctaagtata
    19801 ctaatattaa tattatatca taataaaaag ataatatagt atgatagtat tctaagaatt
    19861 tatcattaat gttgaaaatt aacttgttca aatatataat attaaaagca aagcaattag
    19921 actagaaaag gagtaatgtt gatggaacaa aattatacta aaatagatca tgaattacca
    19981 ttaattcctc taagaggatt agctatattt ccatatatga tattaaattt tgatataggt
    20041 agagagatat ctcttaaagc tttagaccaa gctatgatgg atgaagaact tatattttta
    20101 acttctcaga aagaagcaga ggtagatgag ccaggagaag aggattttta tcatgtggga
    20161 acaatctgta aagttaagca aatgataaaa ttaccaggag atacagttcg tgttttggtt
    20221 gaaggtgtat caaggggtag agtaaagaaa atagaacaag aagatgggta tttcagagca
    20281 gtaatagaag aaatagtatt tgatagtgat aatttggata gtgaaacaga agttgaaatt
    20341 gaagcatttg taagaaatgt atttgatgcc tttgaagagt atataaatat tggaaatagg
    20401 gtatcaccag aaatattaat atctttagct gatatagaag atgtagatag atttatagat
    20461 acgatagctg caaacatata tctaaaatca agccaaaaac aagaaatatt agaagaattt
    20521 gatattagaa aaagacttga acttatttat tctatattat tagaggaaat agatatatta
    20581 aaaatagaaa agaaaataac tttaagagta aaaaaacaga tgaataaggt tcaaaaagaa
    20641 tattatctta gagaacaatt aaaagctata cagaaagaat taggtgaaga agaagatata
    20701 aactctgaag cagatgagta tagagaaaag cttaaaaaaa taaaagcacc taagacaact
    20761 aaagagaaaa tagaaaaaga gatagataaa ttttcaaaaa tatcttctat gtctccagat
    20821 gtatctgtaa gtagaaacta tttagatact atattctcat tgccatggaa taaagagaca
    20881 aaagataaat tagatataac taaggcaaaa gatatattag atgaagacca ttatggatta
    20941 gaaaaagtta aggagagaat actagagtat ctagctataa gaactcttgc taagtcactt
    21001 aaagggccta taatatgtct agttggtcct ccaggaacag gaaagacatc tatagtaaaa
    21061 tcaatcgcca gagcattaaa tagaaaattt gtaagaatat ctttgggtgg agttagagat
    21121 gaagcagaaa taagaggtca tagaagaact tatgtaggtt caattccagg aagaataatt
    21181 aatggggtaa aagaagccca aacaaaaaat cctgtattct tatttgatga gatagataag
    21241 atggcagcag attataaagg tgacccagct tcagcaatgc tagaagtatt agacccagag
    21301 caaaataagg attttgtgga ccattactta gaaatacctt ttgatttatc aaaaatacta
    21361 tttgttacaa cagcaaatag tctaggtaat ataccaagac cattattaga tagaatggaa
    21421 gtaatagaag tatctggata tatagaagaa gaaaaattaa atatagcaaa aaaataccta
    21481 cttccaaaac agattaaaga acatgcttta aaagaaaact ttataaaaat agatgatgaa
    21541 actttaagaa gtataatcaa ccattataca agagaagcag gtgtaagaac actagaaaga
    21601 actattggta agatttgtag aaaggttgcc aaaaaatacg ttgaagaccc aactcttgaa
    21661 gaggttgtta taaataagtc tgacctagag acatatcttg gaaaagacat gtttaagtat
    21721 caacttgcag aagttaaccc tcaaatagga ttagtaaatg gtcttgcgtg gacagaagtt
    21781 ggtggagtta cacttgaagt agaagtaaac gtacttaaag gtaaaggcga aattgtatta
    21841 actggaaaat taggagatgt tatgaaggaa tctgcgaaga caggtatctc ttatataagg
    21901 tcaatagtgg acaagtttga tatagaccct gaattttata agacgaatga tattcatata
    21961 cacattccag aaggtgctgt tccaaaagat ggaccatcag ctggtataac tatggctctt
    22021 gcagtaatct cagcacttac taaaagacct gtgcctggaa atatagcaat gacaggagag
    22081 ataactttaa gaggtagagt cttagcagta ggaggagtta aagaaaagtt attagcagct
    22141 catagagctg gtataacaaa agtattaatt cctaaagaat gtgaagctga tttagatgaa
    22201 ataccagaaa atgtaaaaga aaaaatggag tttgtacttg tagaacatat ggatgaagta
    22261 ttagaacaag ctttattaaa gagtggtgaa aataatgaaa attagaagtt cagagataac
    22321 tatgagtgct gtgaataaat cacagtatcc agcagaagga atcccagaga tagcattggc
    22381 tggtaggtca aatgttggaa aatcatcaat aataaataca ttgcttaaca gaagaaactt
    22441 tgctagaacc agtcaaacac caggaaaaac tagaactata aatttttatc taataaataa
    22501 tgaattttat tttgtagacc ttccaggcta tggatatgca aagatagcta agtctgaaaa
    22561 agaaaagtgg ggaggtataa tggaacgata tctagaaagt agacaagagt tatgttctat
    22621 attcctactt gtagatatta gacatgagcc tacagcagat gataagctta tgtatgaatg
    22681 gataaagcac tttggatata actgtgtagt tattgcaact aaggcagata aaatatctag
    22741 aggacaatat caaaaacaca taagtataat aagaaaaaaa ttgcaaatgg aaagtagtga
    22801 aaaagtaatt cctgtttctt cactaaaaaa gactggtgtt gaagaattat gggaagagat
    22861 tgtaaatcaa tacaatcaac atggatatga aataacagta gattaaattt aaaatgttaa
    22921 atattgacat aagatttatt tgaataataa atgtaataat agttagtttt aattatatta
    22981 atattaaagc aaaatatagg atgccttaaa atagttttaa tgaacttttt ttggcatcct
    23041 tattgtttat aaacaataaa tatcagtaat tttgctaaat actaataaat tttagtaagg
    23101 tatgggaaaa ttagtataaa ttagttcaac taaaaaatta ttgacttatg tagtcagcaa
    23161 taaatagcat ttaagaaata gatttatctg tttttctcat aaagaaaaca gttagtataa
    23221 gccctataaa acatactgat gctgcaatcc aataagactg attgctagct ataacatagg
    23281 ctgatgtgtg acttttaaac ttttgattgt atgtaagact ttgtgcaaaa aaactactaa
    23341 aaaatgctgt tccaagagca aatgataaat tacgaatagt tgcaagaaaa ctgcctccgt
    23401 atccctgttt ttgaggagga acatctgcta aaatactact attgtttggt gaactaaatg
    23461 ctcctagccc aagtccaaaa ataactaaac atacaattaa aagatacaat ggagaatctg
    23521 cttttaaaag tgccattcca actagtgaga tggtcataaa tgaaaaagat gctgttagaa
    23581 tgttttttgc accgtgttta tcagaaagtg aacctgcaat aggtgctata agtaccataa
    23641 taagtggatg tacagtcatt acataaccag ccatcatagg agataaattt tttaattgtt
    23701 ctaaataaaa tggaagtaga aaagatactg ccatttgagg aaaataacaa gctactccaa
    23761 tgatattgcc tacagtaaag tttctatttt taaatagagg taggtcaata aagctttgtt
    23821 caaattttac ttctcttaat gccaacaata agaagattat aagtgctgtt atacctaaat
    23881 acaatcttgt attacccttc tcattcattg caagtaatat aagcgtagtg caagatgcga
    23941 aaagtagaag acctggaata tcaaagctct tgttttgttc atccaaaaca ggtttaggca
    24001 gaaatttaat gccaagaaag actgctataa taccaatagg gatattgata ataaaaatag
    24061 aaggccatcc aaaatgttct aaaattatac ctccaattac aggaccactc atatttccta
    24121 taccaactac agctccagtg attcctatag cttttccttt ttcatttgct ggaaatgcat
    24181 tggaaactat accaagccca gtagccatta aaatacttgc tccaactgcc tgaaagattc
    24241 ttgaagaaat taatgttgat aaattgtttg acatactaca cagaaaagaa cctattgtaa
    24301 atataaaaaa tcctaatgtg tatagacgat ttgaacctac tttatcgcca agttttccga
    24361 aaaataacat agtagctgtt acaacaagca tataagctgt tacaacccac tgaatttgtg
    24421 acatgttaat tttaaagtca gcagcaattg taggatttgc gatatttagt atacttccat
    24481 caagagtaga cataaataca ccaatacaga tgataatcaa tactatccat ttttgtttca
    24541 tgataacact ccaatcttaa tagattttat aagtacgata taattgtaaa ataaacaaca
    24601 aaaaaagcac ctgtatatta ggtaccttga atgtgtctaa tttcatgaat aaatacggta
    24661 gttgaaggat ttgtatagat tactcatgta aaattacata aaggtactca tatactattt
    24721 tcttctacca tccagatttt actgttggct ttggaatatg accaaattat gccataaggc
    24781 tcgcgggctt ttaccgccga tagggaattt cacccgttta tgaagaaaaa cagatgtatt
    24841 ttgtaatatt attttaacat aaaattataa tggaaacaat tggatttaag taatatccag
    24901 ctgtattaca atacaaatat ttcaatatgg acacttaaat tgtatttata gatagaataa
    24961 ggcacagtat gagaatctat aataattaga taaaattaac ctgttattag tattagatgg
    25021 caagttttaa atttgtcaaa ataagaaagc tgtaataaaa ttcttataat atgaatatct
    25081 taattaagag atattgtaaa ttatgaggag gcgctaatat ggaaggaaag gtaaagaagg
    25141 cattcccggg tggaaataca tcacaaggct catattcttt ttttgactat ataattcctg
    25201 aaaatgtaaa aagagttttt tgtttaaagg gtggacctgg agtaggaaaa tcctcactta
    25261 tgaaaaaaat ttataaagag tttttgcata gaggatatga tattgattta tatcattgtc
    25321 cttctgaccc aagttcgttg gatggtttag ttataaagaa attaggcgtg gtacttatgg
    25381 atgcaactgc cccacatact atggacccaa agctacctgg agctttggat gagataatta
    25441 atttaggtga ttactggaat acagaagaat tagaaaagaa taaacaagaa atatcagttt
    25501 atgataaaga tattagtaat tcatttaaaa gagcatttaa gtttttaaaa tcagcagaac
    25561 caatctttag agacatagaa gaaaaatatt ctgattgtat ggattatggc aaagtaaatt
    25621 tggtcacaga agaatttata aaaaggttat ttgatggagt taaaagcaca ggaaatctaa
    25681 aaagagaaaa gcatttattt ggttcagcta taactcctgt agggtgttta gattatacag
    25741 aaaacatttt aaaagatgta aagaaagtct attatttaga tggagaaata ggaactggga
    25801 aaactacatt attaaacaaa gtatataaga aagctattga aaaaggttta aatgtagaag
    25861 tttatcatta tccacttatt ccagagaaaa ttgaaactgt actattaact gatttagaca
    25921 taggtataac tataagtacg acttttaaag aagaagatac aattgattta aatcagtttt
    25981 taaatagaga aaaactactt aaatatgaag aagatattaa atttgatgaa aaagttttag
    26041 atgatttaat ttgttgggca gtactaaatc ttaagagagc caagtctaaa catgatatta
    26101 tagaaagtta ctatagtcct aatatgagat ttgatgaagt agggaaatta agagataaat
    26161 taattaaaag aattttaaaa tatgaggaaa acttataaac gtatatgttt aaaattaatg
    26221 ttaataatga tataattttt agtaaattgg tgtagctttg aggctatgcc attttacttt
    26281 tttttgctaa aaaatgatat taataatctc caaagcaatt atttgttttt cataattatt
    26341 atttcctttc ctaatttata tttttttata atatcaaaac aatgtttatt gaaagtttat
    26401 ataattttaa gaacatctaa aaatgcatac ttgatattgg taaattagtg ataggagttc
    26461 aatgtatgat attatatact aaagatgaat tttaatatat aactttgata taataagcat
    26521 atgtaaatgt ttaatattta attaaagaaa attatcatat aaaaataaat ttgtaaggag
    26581 caatacagta tgaaaaaaca atatgtgttt ataatactaa caagtactat tttaggtata
    26641 cttgttggaa ccatatttag agattatagc aatgaagaaa cagtttatac aagcaaagac
    26701 aagtcaataa gaaaagaaat aaaaatgaca agaaaatcta taaaagaatt aaccaaagag
    26761 aaagacaagt tagataaaga aattaattca atgaaacaaa atcaaaaaaa ttctgaagaa
    26821 atatcagaaa tagaaacact tagagatagt ctatcatatt cagatgtaaa gggtagtggg
    26881 ataatattaa gtatagatgc tatagatgaa aaaacaggga atatagctaa tagtatagac
    26941 tataataaga ttcttgtaaa agttataaat gaattaaaag taaacggagg aaaatacata
    27001 gaaataaatg gtcaaagaat taatcaatat tcagaagttg tacttgcagg aagccatata
    27061 aatataaatg gagttcctgt agcacaacca tatgaaataa agtgcattgg agataaggag
    27121 aaactatcta attatgtaga taaaggtagt aaatatttag atagcataca aaccaattat
    27181 ccaatgaaaa taagcataaa aactgaagaa aatatctcta tgaaaaaaat gaatatacct
    27241 aataaattaa agtatataaa gggtgattaa gattatatta gaggaaagca gtttgccaat
    27301 gttaatacca gaaaaacttg caaaaaaata taatgtattt ccagttgagt ttcaagatga
    27361 taatttagtt gtagaaatag aagatgagga tatttatgct ctacaggact tgagattagc
    27421 tacaggtaaa gaaattatat tgaagaagga gaaaaaagaa cttataagtg aaaagattga
    27481 aaaatattat aatagctttg aacttgatga ggattatgca aaaaaattat ttgaaaatct
    27541 tttagaaaaa gctataaaag aaaacgcaag tgatatacat atagaaccat ttaaagaaca
    27601 tttaatagtg agaatgaggg tagatggaga actaaaggaa gtatcaaagt ttttagttga
    27661 tgtctatcca tcattgtcta cggttataaa attaaaggca tcaatggata taacagaaaa
    27721 aaggcttcct caggatggta gggtggatat aaagttaggt ggaaatttaa ttgacattag
    27781 agtttcatca ataccaactg tctatggtga aaaaatagtt ttgaggatac ttaatagaga
    27841 tactttttta aaagacaaat tagaacttgg tttttcagag gaagctataa aatctataaa
    27901 tcgtataata aataaaagag caggaatatt attagtgaca ggtcctacag gtagtggaaa
    27961 gactacaaca gtatattcct tattaaatga tttaaaagga ataaataaaa atattatgac
    28021 tatagaaaat cctgtagagt ataagatgga tggcataagt cagattcaag taaattcaaa
    28081 agtagggttg agctttgatg taggtcttag agcgatatta agacaagacc cagatataat
    28141 aatggtcgga gaaataagag atgcagaaac agctaagata gcagtaagag cagctataac
    28201 aggtcattta gttataagta ctttacatac taatgatgca gtatcttcca tagctagatt
    28261 actagaaatg caaataccac catatctttt aaatgcctct cttataggtg ttatatctca
    28321 aaagttggtt agaaaagtct gcaatcattg tagccatgag attatgataa aagataattt
    28381 tagtggagat gtaaatacta aggtagctgt agggtgtgaa aaatgtaatg ataaaggata
    28441 ctttggaaga acagcaattt atgaaatctt agaaattaat gatgatataa agacatgtat
    28501 aagaaatatg gaggattcta gtactattaa agatgtggca caaaataatg gaatgataac
    28561 atttgaagat agttgtaaaa gactaataaa tgaaaaaata acgacattag aagagtgttt
    28621 agtagtaaat gaaatgattt aactgaattg tatagttaaa cttcgtttgg tcagttgtct
    28681 ttatttaatc tattttaatt aagatgtttg agtataaatt atatttaagt gaaataaaat
    28741 taaatgcatt aaattagaaa atattaagtt tatttaattg taattaatat aatttggaag
    28801 gtattatagt acatgagatt atctaaaaat atattttata atttagaaaa gttttattta
    28861 gtattaaata gaatcagtga taaagaaatt agagtaattt gcaaagagat aggcatattg
    28921 ctggaatctg gatgtgaaat tacaaaaata tttgagatca ttgagagtca gtcaagtaaa
    28981 aaagcaaaga atttgcttag tatagtatca aatcatattc aaaaaggtaa ctcaatagca
    29041 gaatcatttc aaataacagg tatattttct aaattcttta taagtatgat aaaagcagga
    29101 gaaactagtg gaaacttaga tataataatg agtgatttat ctaattatta tgataaagaa
    29161 tacaaattaa aaatgaaaat aataactatt tcaatatatc caataatact tataatatta
    29221 tctatcttat caatgttgtt tatattcgta tttgtaatac ctaattttca ggtggttttt
    29281 accaacaatg gaattgaacc acctttaata actagagttt taatgggaat atctacagtg
    29341 gttacaaata acttagctta tataatattt agttttatat tgttttcaat aggaagcatt
    29401 tatttcttta taacaaatga taatataaaa aaattgatta attcgttaaa attcaaaata
    29461 ccttttataa aaaaaattaa tcagttagta gctacaacaa gattttcaag ggcattgtcc
    29521 gttttaatta gcagtggaat acaaattgtg gaagctatag acatgtcagc tagtgttata
    29581 gacaacgaat ttatttatga aaggttaaag atttccaata attatataaa aaaaggaaat
    29641 agcataggta aatctttgaa tttagcaaat gtttttccaa aattatttat ttcaatggca
    29701 aatataggag aagaaagtgg gaaactagat aaaagtctaa atgtaataaa taaattttat
    29761 gaaaatgaat taaataacaa aatagagcaa ataatgaaat ttatagaacc tatgctaatt
    29821 gtatttatgg gattaattat tggtactttt attttagcaa tggttgttcc tatgtttgat
    29881 gcaattacta atttttagag agaaaaataa attttattag atgaaacttt tgaaaaggag
    29941 agaatattta attgataaat ttgataaata aaaaacgaaa aggatttaca cttgttgaaa
    30001 tgattgtagt agtaactatt ttaggcgtta tatctagtat agcattagtt aagtatagta
    30061 aggttcaaga aagtgccaaa ttaaatgcag actatacgaa tgctgctaat atagtaaccg
    30121 cagctagcat ggcaattaat gatgatgaaa agacaataga ctctctaagt gtagagacat
    30181 tgaaggaaaa gggataccta aatactgttc cagttcctca gagtacatca ggtaaattcg
    30241 aacttgtcat aaatgatagc ggaacagata taagcgtaaa tataaattct aagcaatttt
    30301 atccaaaata atattttata ttgattgctg taagttttaa aattattttt atttaagaag
    30361 gagatggcta taattaaaaa aattctaaac ttagaaataa atgatgatta tataaggctt
    30421 gtatttttaa gaaaaggtca taaaaaagtt tttatagata aatcaattat aaaagagatt
    30481 aactttgata taaaaaatga tagtaattcg attgagaaga ttaagtatat agaagatgta
    30541 gttagatttg taaaagagga gattggtaaa tcaaaaatat tatataaaaa tttatacttt
    30601 aacattcaaa ttcaggatat agtcattaga aatgttgaag ttctaaatac taagaaaaaa
    30661 agagacatat taagcatgat tgaatttgaa ataacacaat acattccaat aaacataaat
    30721 aactattcaa taaaatataa agttataaat tctaaaaatg ataaattgaa tatacaagta
    30781 attcttttcc caaagagtat tattcaatta attaagaaca tttcagaaaa tctaaatatg
    30841 aatccaaaga ctataaatat aaattttgat atattacaaa agcttataaa cttaaattta
    30901 atagagaatt tttcagaaga aggggtattt atagaatgta aaaaaagtca gttaattata
    30961 aatataacaa aagataaaaa aataagagaa acatacttgc tatcaaaaga gggaaaatca
    31021 tatgagttca taaaaagatt attaagggat tttaagtatg tttattacta tggtatagaa
    31081 aattgtttta ttgaagaata ctttaaatat acagataaat ctattgaagt aaatccatta
    31141 agattaaaag ataaagtaaa agtagaaaat aattttgatg cagatttaga agacaatagg
    31201 ataaattata taagtaatat aggaatgatt atttaagtga aagtggataa aacaataaat
    31261 tttcttaatg gatttaaaaa aactaaacca tgtaataaaa tctataaaat aataatagtc
    31321 ttaattttaa cgtgtatttt atttaaatca tttatgtatg tgaaatctat aaatgaactt
    31381 gaagattata taagtcaaaa taagctagtg gataaatcaa agaatacttt accaacaaca
    31441 gataaaacta gagagaaatt ttctaaaata aaacttaatg aaataaaaaa aatatatgaa
    31501 acaattggaa taaatagtat tactaaaata tcgatagata aaaataagct agagatggaa
    31561 ggtgtgtgta gtgatttaga tgttttaaac aacctaagga aaataaagta cataaaatat
    31621 tattacatag acagtattgt aagagatgga gaatattata gatttagaat aaaaagttag
    31681 atgggtgatt atttttgaga ttaaataata gagaaaaaaa tctacttaaa atattgttaa
    31741 tattagttat tatattggca agtgatatag cagtaagtta tttggaagaa aacctaacta
    31801 gtttgaaaaa tgaaaagcaa aatataataa ataaaataaa taatcaagat aattatcaaa
    31861 taaaggataa taaacatata gatgataaat atatagaaag tgttgaatca aataaaaatg
    31921 aagaattgaa aaataatttt aataactcat ttttagataa aaaaacaaat aaagatatag
    31981 tgttattagt agaaaatgaa ctgagagata ttgtgaatgt agaatatatt aatcaagaaa
    32041 gtagtttaga taatcacaat aaaaaacaag ttaatttaga aataaaagtt aatggagaac
    32101 ttgcaaatgt gttgaaactt gaaggtgtaa tgaagaagtt aagactaaat aataaacttg
    32161 aaaagataga aattaataaa ttaaaagtca ataattttga tgttcaaaat attccaaatg
    32221 aatataaaca agaaaaagac aatattgttg aatgtaaaat gacagttaga gtcatataac
    32281 aaaaaagaat agataaagta aaaaactatt atcaattaat aaaattttat agaggaatta
    32341 gttttacgat aattcatttc tacaagaggg attgttgatg attttatcga aatcctaatt
    32401 aaagttaata tattgtacca aaatataagt gaatttaata gaatatttac ttatactata
    32461 gattgattaa taaaaaaatg gtataataac aaattgtact ttaatttaat aataagggag
    32521 tggaagtcat attgttaggg agaaatgagc tatgcccttg tggaagtggc aaaaaataca
    32581 agaaatgttg tttaaataaa gatattgttt cagaaagagc atgcagaaaa actgaacttt
    32641 cacaaaaaca atatacagat ttatattcaa agttatatga ttattcaaga caggataagt
    32701 ttaaagaaga atgtgaaaaa gctaaagaaa tgttttatat aatgcaaaat gatagtgtaa
    32761 atgaaaaatt tgaaagattt tttaatactt atttaataca agaccacata atggaaaata
    32821 aaaaggttat gacagttggt tttcttgaag aaaatggtac aaacctaagt caatcagaag
    32881 tatcaatatt aaaaagtatg tttgaatcat acgtaagtat ttatgaggta aaagaagttt
    32941 ctgctggaaa aatagtatta aaggactgtt taagtggaaa tgaactttgt acagaagatg
    33001 taaaacttct gagaagtttt aaaatcggaa gttgtatgat agctagaata gttgatattg
    33061 aaggaactaa catattgata gatataacta taagtatatc aaatgaggtt aaagatataa
    33121 ttttaaagga tattatgaat ctattcaatc aatatcaaga tgtatacaaa gatatgaagt
    33181 cgttcttaat ttatcataca catatactat acaagtatat acaacagctt ttagacccaa
    33241 gtatagctga atatttaaag agagaaagag ataaaaacga tacaaaagtt aattcagaaa
    33301 atgaattagc tgaagaagaa tgtaaagttt tagatactct taaagaaaat gtagaaaaag
    33361 aaaatataga tgcatgtata gagttttgga atgaatataa gaaaaatcat gaaaatataa
    33421 aaggagctga aaatggttgg gcagctgctg ttgaatacta tgttaaaaaa gaagcaggac
    33481 aacctataac tcaagttcaa atatcaaaga aatacgatat aagctctagt acactaggaa
    33541 agagatacaa ggatttaaaa atatcttaat taattataaa aagctttaag tcatagataa
    33601 agaatctata gtataatata tgtataatgt ttagaaagag gtattaataa tgagtcttag
    33661 tttaggaaat gcaattgctg tatcaacgaa ctttataaaa tctaacccag ctgtacctgt
    33721 gtacaacgga gaagaaatat atgcagttat ttatcatata gaaataggat ataataatgg
    33781 tgtatcacct gataccaaac aatatataat aatagactat aaagagtttg atttatctat
    33841 ggataaaaaa aatgaactaa taaatattgc taaagagtac tgtaaatcta aaggtattgt
    33901 agaaaacgaa aaccttgaaa taaacctttt agacaatgaa gaagcagaga acttcttaga
    33961 aaaggtgttt aataatatga aggttataag aggtcttata actaaaaaag gataaataaa
    34021 tggactatat aggaatagaa aatataacac cttatgaaaa tacatatgaa tttagtgtat
    34081 atgaatatga tgatgaaatc accttaggta gtgaaaagtt atatgtatgt gaattgaggg
    34141 ttgtattgat taaagttaat tctctgtatg ttgaaagatt gcataaatca gttgaagcaa
    34201 tggtcttagt aaaaaacttg aaaaaagatt tagataaaac acttgttgta aacaaaataa
    34261 agaattttgt gctagatgag atttgggtag aaaatctagt aaaagagaat atagaagtta
    34321 tatttgtaga aagctaggaa taaccctagc ttttttatat tacaatctaa gagaggaaaa
    34381 caatatgtta agagtatcta atgtaaaact tggaattgat aaagatatat ctttattaaa
    34441 agatgttata cttaagaagc taaaaataaa agaaaaaaac ttagtcaaat atagtatata
    34501 taaagaatct atagatgcta gaaaaaaagg aaaaatggaa tttgtttatt ctgtagatgt
    34561 agaagttaaa gatgaaaata aaatacttaa ctcaaaaata aaagatgtaa caaaaataaa
    34621 ggaaatcaga tacgtaaatg tacctgtggg tgataaaaaa ttaaagaata atcctttggt
    34681 aattggtagt ggaccagctg gtttgtttgc aggtctgctt ttagctcaaa tgggttatga
    34741 accaatcata cttgaaagag gtcttgatgt agacagtaga accaaagata taagtgattt
    34801 ttggacaact agaaaattaa aaaataattc aaacgttcaa tttggagaag gtggagcagg
    34861 aactttttcg gatggtaaac ttacaactag aataaaagat atacgatgta gaaaagtatt
    34921 agaagagtta gttcagtttg gagcaccaga tgaaatatta tactcacata agccacatgt
    34981 tggaacagat atattaaaaa atgtagttaa aaatataaga aatgaaataa taagtttagg
    35041 tggtcaagtt agatttaatt caaaagtaac agatatagtt gtatcaaatg attctataca
    35101 atctgtaata ataaatgata ctgaaaaaat agatacagat acaattatat tagctgttgg
    35161 acatagtgca agagatactt atgaaatgtt gtattcaaga ggtataaaaa tagttcaaaa
    35221 accttttgca ataggagcta gagttgaaca tccacaagat ttaataaata aagcacagta
    35281 caaggatttt tataatcatc ctaggttagg agctgcagat tatagactta tagagcatgt
    35341 ttcaaatgga agaacagcat atactttttg tatgtgtcca ggaggtagtg tcatagcatc
    35401 tgcatcaaat gattttgaaa tagttacaaa tggtatgagt gaacatgcaa gagataaaat
    35461 aaatgcaaat agtgcatttt tagtaaatgt aacgccagaa gattttaaga gtgataattc
    35521 actagcaggt gttcatttcc agcagaagta tgaaaaatta gcttttgaat taggtggaaa
    35581 aaattataat gcaccagttc agcttgttgg agattttata aacaataaga cttctttgaa
    35641 tttaggaagt gttgagccat catatagacc aggatataca cttacaaatt taagtgaatg
    35701 tttgcctagc ttcgtaacag agactatgag agaagcgttt atatctcttg ataaaaaatt
    35761 gaatgggttt gcaatgtatg atgctgtttt gacgggagta gaaactaggt catcatcacc
    35821 aataagaata gtaagagatg aaaatacttt agaatcagtg agtattaaga atctatatcc
    35881 atgtggtgaa ggtgctggat atgcaggtgg tatagttaca gcagcagttg atggtataaa
    35941 atgtgcagaa aaaataatac aaaaatacac caataataca aaaatttaac ataaacttaa
    36001 tgatttttta atatttaaca tgaatttaac attatcactc ctataatttg ataaaataca
    36061 ataggttata aaatttttag atacagtaat ctctttacaa ggaggaaata atttgaacat
    36121 tataataagt ctaatgggtg gattaggtct gttcttatat ggtatgaacc ttatgggaga
    36181 aggtcttcaa aaatctgctg ggacaaagct aaaaaagata ataaaactac ttactagtaa
    36241 cctatttatg ggagttttgg taggtacagg agttacagca gtaatacaaa gttcaagtgc
    36301 tactactgta atggttgttg gtttcgtaaa tgcaggaata atgactttaa aacaggcaat
    36361 aggagttata atgggtgcta atatagggac tactgttaca gcccaattgg tatctttcga
    36421 cttaactggt atggccccag tagcattagg tgtaggtatt atactttatt tatttggaaa
    36481 taagcctaga ataaaaaata tagccgaaat acttataggt tttggtatat tatttacagg
    36541 tatggatttt atgaagatgg cagtggagcc actaagagac tatcaaggat ttactgactt
    36601 acttgttaca tttggtagat atccactact tggattatta ttgggatttg ggattacagc
    36661 aataatacaa agttcaagtg catctatggg tatgttggtt gcactggcag cagaaggtct
    36721 agtgccatta tcagcagcac tgccaattct ttatggccaa aatattggta catgtgtaac
    36781 ttcactgtta tcaagtattg gtgctaacaa aaatgcacga agagcagcta tgatgcatct
    36841 aatcttcaat gttttaggaa cagtgatatt cttaatattc ttgaataaac cagtagtaag
    36901 catggttaca agttgggacc caagcaatgt tgcaagacaa atagccaata cacatacatt
    36961 gtttaatata atatctgtat tgatactttt accatttaca aatcttatta taaaactagc
    37021 tataaagctt gttcctgata gagcaggtga tatagatgaa gatgaaacta aaactattaa
    37081 atatatagat gatagaatga tagaaactcc ttctatagct cttgcaaata cgataaaaga
    37141 agcacttcgt atgggtgaga aggcgaagga aagcctaaat gcatctatgg aagctttagt
    37201 agaacattca actgaaaaaa tagataagac atataggcga gagagattaa tcaatgattt
    37261 acagaaagct attttaaatt atttacttaa actttcaaaa gcacctttag atgatgattc
    37321 aagagaagtt gtggatactt tgtttaatac agtcaatgat atagaaagaa ttggtgacca
    37381 cgctaaaaat atagctgaat tatcgcaagt tgcaatagat tcaaatattt ctttctcaga
    37441 agaaggacaa tctgaacttg atgttatgta taatagagtt gtttcagcat atacttatgc
    37501 attagaatca atgagaactg ataatgttga tttagcttgt aaggttataa aaattgaaga
    37561 acaagttgat ataatggaga aatcttgtag agctaatcac atgtatagat tgaacaataa
    37621 tctatgtagt atagaaaatg gagttattta tttagacgta ataagtaatt tagagagaat
    37681 atctgaccat gctgtcaata tagcacaaca agttatagca aagagattag gaaatgatta
    37741 aaaataatta atttaaaata atataaaatt taaaatagag gataaataaa agagaaactt
    37801 taaatgatac gtttagagtt tctttttttg taaatttatt tgactaaaat ttttaaattt
    37861 atacatagtt atagcattta gaagaatcga tttcctgata ataaatgatg agatatgtat
    37921 gattttgtgg caaactttta aaaaaaacac ctatattttg tgttataatc tctttaaaac
    37981 gaatatgctg aaaagaggga tttaaatggg aaaaataaat tttgattata gtaaagctac
    38041 agagtttttt tgtcaaaacg aaatagatgt catgcaacca tatgtagatg ttgcgcacga
    38101 catgttacat aataaaactg gtttaggaaa tactttttta ggatggatag accttcctaa
    38161 aaattatgat aaagaagagt ttgacagaat aaaaaaatct gctgagaaga taaaatcaga
    38221 ctcagatgtt cttttggtaa taggtatagg aggctcttat ttaggttcga gagctgctat
    38281 agatatggta agtcattctt ttaggaatgg acttaaaaaa gagcaaagaa aggctccaga
    38341 agtatatttt gtagggcata atataagctc aacatatatt atggatttat tagatataat
    38401 tgaaggaaag gatatttctg taaatgtaat atctaaatca ggaactacta cagaaccagc
    38461 tctggctttt agaatattta aagattactt agagaaaaag tatggtaagg aagaagcaag
    38521 aaagagaatt tatgcaacaa cagatgcaag taaaggtgca cttagacagt tagctacaga
    38581 agaaggatat gagacttttg taataccaga tgatgtaggt ggtcgttttt ctgtattgac
    38641 agcagttggg ttacttccta ttgctgctgc tggacttgat attgatgcta tgatgaaagg
    38701 cgcaaatgat gctagagaag catttcaaaa tcctgactta aaaagtaatg atagttatag
    38761 atatgctgtt gctagaacaa ttttacatag aaaaggaaaa gatgtagagt tattagtgaa
    38821 ctatgaacct caactacatt atgtaagtga gtggtggaaa cagctttatg gagaaagtga
    38881 aggaaaagaa aataaaggtt tattccctgc ttctgtagat ttctcaacag atttacattc
    38941 tatgggtcaa tacatacaag atggaaaaag gcttttattt gagacagtac ttaatgttga
    39001 aaactgtaag agaaatataa ctataagttc agaggaagta gacttagatg gtttaaacta
    39061 tttagcaggt aagactgtag attttgtaaa tcataaagct tttgaaggaa ctctattggc
    39121 tcatacagat ggaaaagtac ctaatttagt aataaatata cctcaactag atgagtataa
    39181 ttttggatat ttagtttatt tctttgagaa ggcttgtgga ataagtggat atttattggg
    39241 agttaatcca tttgaccaac caggagtaga agcttacaag aaaaatatgt ttgctttact
    39301 aggcaaacca ggttatgaaa aagaaaagga agagctagaa aaaagactta aataatttta
    39361 aatagttaag gtttaattaa gattaaaata atataatgaa atcatcaaaa aggagtttac
    39421 tcaaaataga tgatttcatt ttttttatga aaaattggga aaaatatatt ttgataattt
    39481 atattatatt aagataggga gtggtatgag atgtcaagaa gaaagaaagg gataagttta
    39541 gtaatattag ttgctataat aagttcaata cttagtagtt ttctaacaat aattttagtt
    39601 aaggacaatt tagtaagcaa atctacagga agttctacgc cgatagttgt aaatgatgat
    39661 ggtaagagtc aaaatattta tcaagcagtt gcagaaaaag ctacgccatc tgttgtaggt
    39721 attacaacaa ctagtgttga tactagtaat atgtttgcaa taccaactga aacacaaggt
    39781 gtaggaactg gaatcatagt tgattcaaat ggatatatac ttactaattc tcatgttata
    39841 tcagatggtc aagctacgag tgtaaatgta ttattcaatg atggttcaac aacttcaggt
    39901 aaagtagtat ggtttgacca acaacttgat ttagctatag ttaaggttga taaaactgga
    39961 ctaacaccag cagaatttgc tgatagtgac aaagtaaaag ttggagatat atcaatagca
    40021 ataggtaatc ctcttggatt agattttcaa aaaacagtta ctcaagggat aataagtgga
    40081 ttagatagaa ctatacaaac agaaaaaact aatatgactg gtttattaca aactgatgca
    40141 agtataaatg caggaaatag tggaggacca ttgttaaatc aaaaaggtca agtaattggt
    40201 ataaacacag caaaagcttc tcaagcagaa ggattaggat ttgcaatacc aatcaataca
    40261 gctaagagta tagttgaaga agtaatcaaa aatggaaaat atgaaaaagt aactttaggc
    40321 ataaaaggta cagatgtatc aaattatgaa gcagcaacag gtacaaaatt aagtactgat
    40381 aaaggtgttt atgttgcaga agtaatatct ggttcatcag cagaaaaagc tggagtaaag
    40441 gtaggggata ttataactaa agtaggagat acagatataa caggtatgaa tgatttaaat
    40501 aaaaagttgt atactttctc aaaaggtgct agtacaaaaa taacagtaaa tagaggcgga
    40561 aaagcagtaa caataaatgt gaatttctaa atatgaccag aaagatagag actacatggt
    40621 aaagtgtagt ctctattttt atttaatttt tatagtatat acatctaaat taattaaaat
    40681 gatgtattgt ttatagctta ttgctatatt ttcttataaa aataaattta ataaatattt
    40741 aaatttaatt tacaaaaaca atataaatat cataataaaa taaaattttt taatattact
    40801 aaaaatatat agacaaaata ataacaaata tagtattatg taattgtaga aatgcttttc
    40861 ataggtatat tctatgaaga tgaattgcat aaaaatgaaa aaatggaatg gatattattt
    40921 aggggtgaaa aatcatgaat ccattagtta taaacttaca aaaatgtagc atacatgatg
    40981 gaccagggat aagaagtaca gtatttttta aagggtgtcc acttgaatgt gtttggtgtc
    41041 ataatccaga gagtcaaaca tatacaaaac aagttttgta taatgaagaa agatgttcaa
    41101 aatgtgaagc atgtataaat atatgccctc ataaggcaat ttacaaaggt gaaacaaaaa
    41161 tatgcttaga ccaagataag tgtgagtttt gtgaaacttg cttagactat tgtgtaaaca
    41221 atgcaagaga gattgttggt caagagtatt ctgtaagaga tttggttaaa gagatagaga
    41281 aggatagaat tttctatgaa gaatcaggtg gtggagtaac tctatcaggt ggagaagtaa
    41341 tggctcaaga tatggacttc atttgtggag taataaacat gtgcaaatca aagggaatcc
    41401 atgtagcaat tgatacatgt ggctatgcaa agtcagaaaa ttatgaaaga gttgctaaat
    41461 gtgcagattt gtttttatat gatattaagc ttattgatga agataagcac ataaaattta
    41521 caggaaaatc aaatgactta atactgaaaa atgttaagat tttaagtgaa ttaggggcta
    41581 acataaatat aagaatacca ttaattgtag gagtaaatgt agatgatgaa aatttagagg
    41641 ttaaaaaaat gatagagttt ttaaagcctt tgaatataca agcagtaagt cttcttccat
    41701 atcataatat tggtaaacac aagtatgata aaatttataa aaagtatgaa ggagaagaac
    41761 tacaaagacc atcagaagaa aaattagagg aaataaaaag actgtttgaa gcaagtaatt
    41821 tcaatactaa gattggaggg taataaaatg gcaagaggaa cttttgagag aactaaaaaa
    41881 ttaagagaag aaagtattaa tgcagaacca cacatatcta ttgaaagagc agtacttatg
    41941 acagaagctt ataaaaaata tgagggaagt gtagagattc ctgtacttcg tgctttatca
    42001 tttaagcatt atattgagaa tagaactctt agcataaatg atggagaatt aatagtaggt
    42061 gaaaaaggtg attctcctaa tggagcacca acatatccag aaatttgttg tcatacaatg
    42121 gaagatttag aagtaatgca caatagagat ataataaatt ttagcgtatc agaagaagct
    42181 agaaaaatac ataaagaaga aataattcct ttttggaaaa agagacaaac aagagataag
    42241 ataataaatg caatgacacc tgagtggtta gcagcatatg aagcaggtat gtttacagag
    42301 tttatggaac aaagagcccc aggacataca gtttgtggag atacaatata taaaaaagga
    42361 tttttagatt taaagaaaga catagaagca agattaaaag agctagattt tcttaatgat
    42421 ttagatgcat ataataaaaa agcagattta gaagctatgg caatagcttg tgatgcaatg
    42481 gttatattag gtaaaagata tgctgagaaa gcaagacaaa tggcagaaga agaaacagat
    42541 gaagctaaga aaaaagattt attactaatt gctgaaactt gtgatgttgt accagctcac
    42601 aaaccagaaa cttatcatca agctatacaa atgtattggt ttgttcatat aggggttact
    42661 actgaactta atatatggga tgcttttact ccaggaagac ttgaccaaca tctaaatcct
    42721 ttctatgaaa gagatgtaga aaatggaata ctagatagag atagagcaca agaattactt
    42781 gaatgtttat gggttaaatt taacaatcag ccagcaccac caaaagttgg tataacatta
    42841 aaagaaagta gcacatatac agattttgca aatataaaca ctggtggaat aaatccagat
    42901 ggtcaagatg gtgttaatga agttagttat ataatacttg atgttatgga tgaaatgaaa
    42961 ttgatacaac ctagttcaaa tgttcaaata agtaaaaaga ctcctcaaaa attcttaaag
    43021 agagcttgtg aaatatcaag aaaaggatgg ggtcaaccag cattttataa tactgaagca
    43081 atagttcaag agcttatgga agctggaaaa actatagaag atgcaagact tggtggaacc
    43141 agtggttgtg ttgaaactgg atgttttggt aaagaagcat atgttctaac tggatatatg
    43201 aacattccaa aaatacttga attaacatta aataatggat atgacccaat tagtaaaaaa
    43261 caaataggta ttgaaactgg agaccctaga aatttccaat cttatgagga actatttgaa
    43321 gcatttaaaa agcaattaca ttatatgatt gacataaaaa tagaaggaaa tgctgtgata
    43381 gaaaatatat gtgcaaaaca catgccttgt ccacttatgt ctacaatagt tgatgattgt
    43441 atagaaaaag gcaaggatta ccaaagaggt ggagcaagat acaacactag atatatacaa
    43501 ggtgttggta taggaactat aacagatagt ttaacagcaa taaaatataa tgtatttgat
    43561 aagaaaaaat tcgatatgga tacattgctt aaagcacttg atgctaattt tgaaggatat
    43621 gaagctatac ttaatttagt ttcaaataag actcctaaat atggaaatga tgatgattat
    43681 gctgatgaaa taatgcaaga gatattcaat gcatattaca atgaggtaac tggtagacca
    43741 actgtttgtg gtggagaata cagagtagat atgttaccaa caacttgtca tatatacttt
    43801 ggagaaatta tgggagcaag tccaaatggt agactttgtg caaaaccagt ttcagaggga
    43861 atttcacctg aaaaaggtgg agatactaat ggacctacag cagttataaa atcttgtgct
    43921 aagatggacc acataaaaac tggtggaaca ttattaaatc aaagatttgc accatcagta
    43981 gttcaaggag aaaaagggtt agataatatg gctaatctag taagagcata ctttaatatg
    44041 gatggtcatc atatacaatt taatgtattt gataaaaatg tactgttaga ggctcaaaag
    44101 aatcctcagg attataaaga cttaatagtt agagttgcag gatatagtga ccatttcaat
    44161 aatttaagta gaacactaca agatgagata ataggaagaa ctgagcaaac attctaggtt
    44221 tgcttgtaaa taaaacttct tactatataa aatcgtaact gatataataa agtataaatg
    44281 gtttgattcc atttttaaaa ttttagatta attatgagct aactttctaa ataggaagtt
    44341 agctcgttaa gtttaatgaa aaaatcgtta aatttatatt tagtaaatca tacacatatt
    44401 agtaagtaat acatacaaaa gaataattta ttatagttat aataaggtta tattttataa
    44461 aaataagata attttaagca gttaaaataa agtcaattta gctactttaa aataaaacta
    44521 atagaaaaaa ggagatttga tatgaaaact ttaggattta ttggttcagg gaatatggga
    44581 agtgcaatga taggtggcat agtacagtca gaattagtaa aggcagaaga tataactgta
    44641 tcagatttaa gccaagttgc cttagataaa gtaaaagaag aatttggagt aaatactaca
    44701 actgattcaa aggtagctgt tacaaattct gatgtggtaa tagtagcact taaacctaat
    44761 atatgcgaaa aggctttaac tccacttaaa gaattaatag atgcaaataa aataatagta
    44821 tctatagcag caggtaaaac tatagaaagt ctggaaagtt ttataggttc agataaaaaa
    44881 atagtaagag taatgcctaa tactccagcc cttgttggcg aaggaatgtc agctctttgt
    44941 aaaaatagta atgtaacaga tgaagagtta aatatgatta aggctctgtt tgaatctttt
    45001 ggtgaggcag aaatagtatc agaatattta atggatacag ttacaggtat tagtggttct
    45061 tctcctgcat atgtatttat gtttatagaa gctatggcag atgcagcagt attagctgga
    45121 atgccaagac aacaagctta taaatttgca tctcaagcag taatggggtc agctaagatg
    45181 gttcttgaaa ctggaaaaca tccaggtgaa ttaaaagata tggtttgttc tccagcagga
    45241 actactatag aagctgttaa ggtattagaa gaagaaggct ttagagcagc agttataaaa
    45301 tctattgttg cttgtataga taagtctaaa gatatgagca aataacagat gtaattaagt
    45361 ttactatatg gtttcttgaa ttgttttttt agtgcttatt tatatctata cataaatgta
    45421 tttggtataa gtaaaataag agtaatttac tataaaaagg aatttaatga ttgagtgaga
    45481 atttattact taaatattaa taattaaata gatatatttt taactatatt tgagatatgt
    45541 cattgtgaaa aaataaaatt tataagtatt attgtgaggt tataagtaga tttatttaaa
    45601 aataaattta tttatagcct ttttaaattt gaaataatat tcataaggta ataaaagtta
    45661 caaatatttt cttcgctaat aagttttaaa atacctataa tgttttactt tttattataa
    45721 taaatacaat aaatatattc ttatataaat tctatgtcag atatatggaa agataagaac
    45781 cttgagatta tgttaaaaaa tctagatttt atacttataa aaagtttgat tttacaacct
    45841 tacaaaagag aatcaagata ggaatataat tatctttttc ataaatatat aaatgatata
    45901 ctaatattat agtattattg atatttgtat tggaggttga acatgaggga tgaaatttta
    45961 agttttgtaa atgaatgtgt attatttaac aactttgatg aacttaaaaa agaaagtggc
    46021 ataataacag aagatatagt aaaaaagttt aatttaagta gaactcaatc aagtagatta
    46081 ttaaatgatt tagtaaaatc tgataatctt ataaagataa acagtagacc tgttattttt
    46141 ttacctaaaa ataaaataaa tgatgtagta ggacctacaa aaaaaagtat ttatgaaaat
    46201 ttagatgaac ttaaaaatga gtgtaaatta ttaaagcaag agactgtgtt taatagtatc
    46261 ataggatatg agaatagctt aaaagaagta atcgaacaaa taaaaaccgc tgtactttat
    46321 ccaaatggcg gacttacaat aatgctgaca ggtgaaagtg gagtaggaaa aactttttta
    46381 gccaagataa tatacaaata tagtgtaagc agtaatgtat tagaaaaaaa ttctccatac
    46441 aatgtgttaa actgtgcgca gtattataat aatttggagt tattgtctag tctattattt
    46501 gggcatgtaa aaggagcata tactggagca aatgaatcta gagcaggtct aatagaacaa
    46561 tcagatggtg gtgttttatt tttagatgaa gtacatagat taaatagtga aggtcaggaa
    46621 aagttattta catttatgga tacaggtacg ttttctagga ttggagagaa tggagtaatt
    46681 agaaaagcta gggtaagatt aatatttgct acaacagaaa gtctatctga atttttacaa
    46741 acctttttaa gaagaatacc cataaatatt tatgttccca atatagaaga aagagggact
    46801 attgaaaaaa gaaagattgt agaacatttt atttatgaag aaagtaaggt acttggtttg
    46861 cctatagaga taactcaaat tacattaaat gcgattttaa agataaaatt caaggggaat
    46921 ataggtgaat gtaaaaatgt aataaaatat gcatgtggac gagcttatgc aaaaaatcaa
    46981 agaagaaatg gaaatatttt tgttactctt caagaccttc ctagaaatct ttatatagaa
    47041 aatgtagata tatttcaatt ccaaagtggt aaaagcaaaa gcattatctt ttctcctaaa
    47101 caatttagta ttgaaatgtc aaatggaaga aataaaagaa atataatcta tcaaagtttc
    47161 ataaaatgtt tagattactt tgatagacta gaaaaaaatg aaataacaaa ggaaaaattt
    47221 agtaaaaaga gttctaatct aatgattaga ctaatggaca atttagtctt ttcaaataat
    47281 gatgaaaata ataacgtact tatggagctt ataatatcga taatgcagga tatatttaga
    47341 aatttagaag ttaataacaa tataaaatat gatgggaata atgtattggc attttcgtct
    47401 tatctttatt tgaaggacga atcttatcaa ttgtctgatg agtataaagc tttagaaaat
    47461 aaattactaa tatatttaaa aaaagagagt tacaaagaat actctataat aagtaaaata
    47521 tcaacattaa taacgaatag attggatatg tatcttaata atgtagatac aatcataatg
    47581 attatattta ttaaatcaac aattataaga atgaaattta atcaagttaa tgcgactatt
    47641 atagcacatg gatatgctac tgccagtagt atggcgaatg tatgtaatcg gatgttgggt
    47701 aaaaatatat ttacagctat tgatatgcct atagatgcta ctattgctga tgtatcaaaa
    47761 caaatgtcta gatacctaga gggagatagg ataaagaatg gtttaattgt gttatttgat
    47821 atgggctctc ttaatcttat atttgaagat ttaaagaata agataaatag cccaatgtta
    47881 tttatagacc aaatatccac tatttctgca ctagaagtag gaaatttatt aatgcaaggt
    47941 aaaaacattg aagagattgc agaaattatg gatgaagaaa ctaaacctaa tatacaattg
    48001 tattatcctc aaaaaaataa agaatatgct ataatatcat cttgttttac agggataggt
    48061 actgctataa aaatccaaca tctactttcc gaaagtttaa ttggaatagt tgatgtcaaa
    48121 ataatacctt atgattatga tagtcttgtt tctatgggag ataaagaacc agtttttgaa
    48181 atgtatgatg tgatggctat agtaggaact gccaatccaa atgtgaatgg ggtagatttt
    48241 atattattag aggatataat atcaggaaaa ggagaagatg atgtttttag aatatttagc
    48301 agagtagtag aaaatgaaaa aataaagaac ataaatgatt ctattgtaaa gaatttttca
    48361 ttaattagag taatagatag tctaactatt ttagattcaa aaaagataat agagagaata
    48421 gaagaaggaa ttaatgctat agaaaatatt caaaagaaaa aactttcaaa tgataaaaag
    48481 atttctttat atgttcactt aagttgtatg attgaaagat tagttagaca aactgaaatt
    48541 gaagagtata ctgatatgga tttgttaatc aaaaaccatc atagtgaagt aaaactaata
    48601 aaaaatgcat ttagtgtgct agaaaagagt tatagtgtgc aaataccaat ttcagagata
    48661 ggatatattt ataatattat ctatggtcta ccacagtaaa atacaagatt acaaaataag
    48721 tgaatgaaat ttaaaaaagt atgccagtat ttggcatact ttttgctttt atagataaat
    48781 gagagtataa acaataaaaa ttttaatgta tgatggagga tttggggaaa atggtatctt
    48841 tgatgagaat tgatgacaga ttgatacatg gtcaggttgc atatggatgg gcaacatcac
    48901 taggtataaa tgtaatacta gttgcaaatg atgaagcaaa aaatgaccaa atgaaagcta
    48961 tgtcactgaa cttagcgaaa cctagcaatg taactttata tataaggggg gtagaggaat
    49021 ctggagaaat agtagaaaaa tttgcttctt caaagaaaag caatgtatta gtgttagtaa
    49081 aaaataccaa agatgcttta gttgtagcta aaaattcagg aggagtatta aaagaaataa
    49141 atgtaggagg attaagatat gaggaaggga agaagaaact tacagattta gttgctgttg
    49201 atgatgatga tataaataac tttaaacaaa taggtgattt agggattgat atagaattta
    49261 gaatgttacc tagagacaaa aagaaacaac tatcagattt aataaaataa tttaggagaa
    49321 agagaatgaa aaaatttcta attgcaacac atggagaatt gtccagagag cttgttgaca
    49381 caagtaaatt aataattggt tcattaacta atattgagta tttttgtatg acaacggata
    49441 aatcagcaga tgatgctgag aaagaaatta gaagtatatt atcaaataat aaagatagtg
    49501 aagaatttgt tgtcttaaca gatgtttttg gtggaagtgt ggcaaacata tgtacaaact
    49561 tacttttaca agggttcaaa tttgaattat tgacaggagt aaatttacca atgcttttat
    49621 ctatactttt attagttgag gaggatacaa aactaatagt taaatcagga ttagaggaag
    49681 caaaaaatgg aattgtttat gtaaatgagt tattagaaaa tcaaaacaaa atcaaaaatg
    49741 taatgtaatt taatgtaaat taaactaagt taaaatggag gttcaatatg ttagtacaag
    49801 ctactatgtt agctattata gcaggagttg gtatactaga tgggcgtata tttggtcaat
    49861 ctatgcttga taggccaata gttacaggta tgttagtagg attagtttta ggagacataa
    49921 aatcaggaat tatgattggt gcacagcttg aattaatttg gatgggaatt gcaggaatag
    49981 gtgcagctac tcctccagat gtagtaacag gaggcgtatt aggtacagct tttgctatat
    50041 tatcaggtaa tggtgcagaa gttgctttag cagtagcagt accagtagca gtcttagcac
    50101 aatctcttgg agtattagtt agaataataa attcgtactt ttctcaaaag gcagttttct
    50161 atgctaaaca agctgatttt aagaaagtta ccataatgat gtggttgcca gtaatattat
    50221 tctttttaag tacatcagtt ccaacctttt tagcaattat gattggtgct gataaagtta
    50281 cagcattgat aaatgcaata cctaaagtta tattagacgg actgggtgtt gcaggaacat
    50341 tgttgccagc agtaggtttt gcattgctta tggatatgtt actttcaaaa aagatgtctg
    50401 tatttttctt tatagggttt ttgttagcat cttatggtgg attagatata actgctatag
    50461 ctttatttgg agtatgtgta gcatttgtac taaactttta tatcaataac aaagaaaata
    50521 atcaaaatcc tcaattagca atcaatactt taacggaagg agagattgac tttgaataaa
    50581 ttaacaaaaa aagaattaag aagtatgttt tggcgttcat ttgttcttca aggtgcattt
    50641 aattacgaaa gaatgcaaaa cttaggttac tgttattcaa tgttacctgc aataaagaag
    50701 ctatacaata agaaagaaaa tcaagcaaaa gcaatagaaa ggcatttgga gatttttaat
    50761 acaacgacag tagtagttcc tgctatatta ggtataacag cagctatgga agaagagaat
    50821 gctaataatc cagaatttga tgaaagttca attagtgctg ttaaaacagc tcttatggga
    50881 cctcttgcag gaattggtga ttcattattc tggggaacat ttaggattat agcagcagga
    50941 gtaggagtat cacttgcaaa agaaggtaat atctttggac cactattatt cttactatta
    51001 tacaatatac cagcatttgc ccttagaata tttggactta agtatggtta tcaagtaggg
    51061 gtaaattctc ttgaaagaat acaaagagag ggacttatgg agaaaataat gtctatggct
    51121 acaactgttg gtttattcgt tgtaggagga atggttgcaa ctatgttgag tataactact
    51181 cctctgaaat tcaacttaaa tggtgcagaa gtaattttac aagatatact agataaaata
    51241 atacctaata tgttaccttt aacctttgca tttgtaatat actacatgtt aaagagaaag
    51301 gtaagtgtaa caaaattaac tataggcact atagtaacag gaatcgcttt acatgctata
    51361 ggtttgttat agaaattaat tataatttat tgaatatata gataaggaag gtgtataaaa
    51421 tgacaataca agattatatg ttagaaacac cagttagaat gagagaaatt atatctaatg
    51481 cagatagctt atttaatgaa gttaagagaa caaatctaaa aaagattata ataacaggtt
    51541 caggtactag ctatcactca ggagtgcaag tgcaaccata tttacaaaat ttattagata
    51601 tagatgttgt gaaaatgtat ccatttatga taactgaaga tacattcaag tttgataatg
    51661 aaaataccct agtagtaggt gtatctcaag gtgggagtag ttattctaca tacaacgcta
    51721 tgaagttagc tgaagataag ggctgtaaaa tagcatctat ggcaggttgt aagaatgctt
    51781 taatagatga gatttctgat tatatattaa cagtaaattg tggtgaagaa aaatctggag
    51841 ctaaaacaaa aggatattat tgtacaaagt taaacttaat gttattaggt cttcaaatag
    51901 ctagagaaaa aggtataata tctagtgaaa agtacaatga ggaaataaat aagatattag
    51961 acgcaattaa tagatttgaa gcagtgtata agctctctaa acaatggatt gaaagaaaca
    52021 aagaaaagtt agttaattct aaagaaataa gaataatagg tcactctgat atatatggag
    52081 atacattaga agcagcatta aaattattgg aaacaatgag aatacctgta actgggtatg
    52141 agtttgaaga gtttatacat ggtatatata atgctataaa tagtgattct actatattta
    52201 tactagatac aggaaaagaa cctagagtaa ctaagatgat tgatgtactt tctggttgga
    52261 cggaaaatgt atttgctata ggtagagatg ttacagaaaa tgataaaaat ttaaagattg
    52321 atatcacaga taatccttac tatcaaacat ttaattttat agtacctata caactaatat
    52381 gtggggaaat acctacatta agaggtgtag acccaagtgt tccaaaagat actaggttcc
    52441 acatgaagtt aggtagcaag aaactgaata agtaacaaaa aaagaggttg tctcaaaata
    52501 gagataagcc tcttttttac tagataagct cttttttact gttgaaatga tgaaaatata
    52561 tttgattttc tataaaaaag agtgcctcat gaactaaaaa gttaattttg agacacccac
    52621 ttttatctta ttttaaacta aaatatttat atattagtgt gcattatttt ttttattgct
    52681 gtcttcctta cttgagcaag aattagcact tggacatcca gaacaaatag aacatccact
    52741 acagacattt ccgttttttg tagacttgaa tttattaaac agaataaaga gtgcaaggac
    52801 tataattatt ccacctatca aagcttctat tattgaatta cccataaata gtactccacc
    52861 tatactttta actataaaag ccattatcca tgctagtata aattgatatg caaatgatgt
    52921 aaacatcatt ttattgccat attctttttt catagttgca agtgcagcta tacatggagt
    52981 atataaagca gtaaatatca agaatgtata tgcagtaact ccattaaata ttgttggtaa
    53041 gacaacagtt agattaccat atagtatatt taaagagttt actacgattt cttttgctcc
    53101 aagaccactt aaaatagcaa ctgaagttct ccaatcatca aaaccaagag gtttgaatat
    53161 aggagatata atatgaccta gagtagccaa gaagcttaca tttatatctt ctgtatatcc
    53221 aaaaacattg aatgaagaaa gtccccaaat tacgactgac attgcaaaca tgacagtaac
    53281 aactcttatc aagaaacctt ttgatttatt ccaagtattt ttaataagtg catctatagt
    53341 agggattcta tattctggta attctaatat aaatggttca gcctctgttt taaaggctgt
    53401 tttatttaag aataatgcaa ctacaattgc cataactata cccaccaaat acagtgatgt
    53461 agttactaaa gcagcattat gtggaaagaa tatagctaca aatagtgcat atataggtag
    53521 ctttgctcca caagtcataa gtggtgcaat aagagctgta actcttctat ctttttcgct
    53581 ttccaatgtt ctagttgcca ttatagctgg agaagaacat cccataccca taactatagg
    53641 tataaaagct ttaccagata gaccaacctt tctcataact ttatccatta aaaatgctgt
    53701 tcttgacata tatccactat cttcaaacaa cgatatacca aagaataatg taaatattaa
    53761 agggaagaat ggtaaagtac caccaacacc tccaattatt ccatcaacta taagtgattt
    53821 aaaccatggg ctagagttag ataacatatc actaacaggt gcagaaatat aagcctctat
    53881 aagactagca aatccctctt gtagtggacc tccgacccag tcaaaggtaa atttaaataa
    53941 gaggaacaat acaccaataa atattggata tgctaatata ggattcaaga ctatattgtc
    54001 tatcttatca ctgatagatt tcttttgtgt atatgtagtt tttgtacatc ttgataaaat
    54061 ggattctaat tttttatatg tttctgtttc acttccaaag tttttctgat atataaaagc
    54121 tttttgcgta gctcccttaa tctcttcttc tatcctatca acaccatttt tcttttttgc
    54181 tataattgga acaacaatta ctccaagttc ttcacttaat tttttggcat ctatattaac
    54241 tcctttagat tctgcaatgt ctaacatatt cagcaatata actataggct tgttaaattg
    54301 cataagttga gttgttaagt aaaggtttct tgataaatta gaagcatcaa ctacatttac
    54361 tattacatca acatcttcat tttctaaata tgatttagaa accttttctt catttgagaa
    54421 agtatccatt gcatatatac caggtaaatc gacaaccttt atctcttttc caagaaatcc
    54481 ttcttttttt tctatagtaa caccaggcca gttacctaca tattgatttg aacctgtaag
    54541 tagattaaaa acagtagttt taccgacatt tgggtttcct aataatgcta cattaatcac
    54601 gggtagctcc tcctctatta ttgtaaagaa atatttttag catcagattt tctaatcgca
    54661 aggtcaaagc ctctaaatct aattataatt gggtctccaa gtggggcaac ctttttaact
    54721 tctatttcag tactatttat acatccaagt gcaagcaatc ttttagataa tttaatatct
    54781 ccatctatac tattaataaa tcctttttct cctattttta aatcgtaaac agtcattatt
    54841 aacacctcct aatgaaattc attatcatta aaattgtaac aagtatgata caaaaagtca
    54901 agatttatag aggtaattgc acattgaaat aactaatatt tattctaaat agattaaaaa
    54961 tattttagtc taaattaggt gtttttatat attttgtatt ataatgtaag atgatagata
    55021 aaatttggag gagttaactt aatgaaaact agaaaaatga catttttagg tcttatgata
    55081 gggtatagtt tagctttata tgtattagag acatatatac caaatcctct tatagttata
    55141 tttccagggg caaaattggg tttgagcaat attataactt taatttcatt aatgttgtta
    55201 ggtgtcaaag atactgttat aattgttact atcagggtaa ttttgtcatc aatatttgca
    55261 ggccctttat catatctact atttagtata ggaggagctt atctaagtct tttatttatg
    55321 tatttagcga gtaaaataaa agatttatca ctaataggga taagcattat aggtgcaata
    55381 gggcataata taggtcaatt aatagttgca agtttaatca ttgaaaatat acttgctata
    55441 gggtacttac catttatgtt aatggcatca ttagtaactg gtatgtttgt aggcttagta
    55501 tccaggtata caatacctaa attaaagcat ttttatagta agttttattg acataatatt
    55561 gacaaaatat aatattgttt gataaaatgt atataaaaat atacaaattt gtaatagtgg
    55621 tgattattat ggcgaacaat gaattagaag cgttaaaaaa tgagattgaa gcagttaggg
    55681 aagagataaa tacatacata gaatatccag aaatatttca agaagagtta gtagaatcaa
    55741 gtaagaaaat agatatatta ataaataaat acatgtacct aagcaaatag cccacagttg
    55801 tgggtttttt tgtatcaaaa attaacagat ggctatattt tacatatatt aataataata
    55861 ttaagatata aaatagtatt tctgatgtgt aaattatctg gaggacataa gttgtaatta
    55921 agtcaggagg gttttatatt atgattgata taggaagtaa ggagatagca ataaggttac
    55981 tattagcact aataatagga ggaataatag gcttagagag agaaaaaata agacagtttg
    56041 caggatttag gacacatata ctaattgcaa taggttcttg tattgtttca ataacttcga
    56101 ttcaattatt ctttgattat aatttgtata caaatgctga cccagcaaga atgcctgctc
    56161 aagtattatc tggaattggt tttttaggtg caggagcaat tttaaaaaat aaaggtggag
    56221 taaaagggct tactacagct gctgggatgt gggcaactgc atgtattggt atagcagttg
    56281 gatatggtta ttatgaattg gcaattctag gtggattatt tgtaatgata gcattatttg
    56341 tgcttaggat aatcaatacg tttttattta gccaagaaaa gaatgtattg atattaaaat
    56401 taagcaattt ggattctata ccttttttat atgataaatt tgaaaaaaat caacttatcg
    56461 taagaaatat ggaaatagaa tgtcgtaatg aagaatactg gaaggttaat ttttttgtat
    56521 cttatgctaa gagaatacac ttacacgacg ttatgaaaga aataaatatg atagaaggag
    56581 taataaatgt agattatttg gactaaaata tatacaatat attttgtaaa gtgaataaaa
    56641 tataaaacaa attaaaatag aggtgtatga tatgggaact gatagaagta caatagaaga
    56701 aaagtacaaa tggaagatag ataagatgta ctccagtaaa gaggaaattg aaaaagacat
    56761 aagtaaagta aaaaatctta tccaagaagt aaaagaatat aaagggaagc tttctgagag
    56821 taaggagaac ttatataaag tattaaatat ctctgaaaat gcatctagga ttgttcaaaa
    56881 tttatatgta tatactcata tgaaacaaca tgaagatact agaattaatg ataatcaagg
    56941 aagagccact aagacagaaa tgttatctac tgatttaggt gtggctactt cttatatagt
    57001 tccagaaata ctggcgatag aagagaataa gttaagtgaa tacttaaaag atgagaagct
    57061 atccttttat acaaaatata taaaggatat attgagagat aaaccacata ctttgagtga
    57121 acgagaggaa gaaatacttg cagctacatc agaattatcg acaattccag aaaatgtata
    57181 cgatatgtta gcatatgcag atatggaatt tcctgagata gaagatgagg aggggaataa
    57241 ggttaagctt tctcattcaa actactcttt atttataaaa agtaagaata gtagagttag
    57301 aaaagacgct tttgaaggtg aattttcaac atatgagaaa tataaaaata catttgcctc
    57361 aacgctttat ggtggaataa aatcagagat attttacgca aaaactagaa agtataattc
    57421 agcaatagaa gcgtctttat tttcagatga tgtaagttta gatgtttaca ataatcttat
    57481 atcagctata gataaaagta ttcctaatct aaataaatat gttgagctta aaaagaaatt
    57541 tttaggattg aatgaaatac acatgtatga tttgtatgtt cctttgacag ataaatttga
    57601 catggatata ccatatgata aagcacagga tatcatatta gaggcactta aacctttagg
    57661 agaagaatac ttgaaggtca taaaaagtgc ttttgatgaa gggtggattg atgtatatga
    57721 taatgaaggt aaaaaaggag gagcttattc ttggggaagt tatgattcac atccttatat
    57781 attaatgagc tataaaaatg accttaattc tctattcact ttaattcatg aattagggca
    57841 ctctgttcac agccattatt caagaacaag tcaaccattt ttatattcgg actataagat
    57901 attcgttgca gaagttgcat ctacattaaa tgaattactt cttataaatt atttattaga
    57961 aaattctaag tctaaggatg aaactatata cttattaaat tattatttag aacaatttag
    58021 aactacagtt catagacaaa ctatgtttgc tgaatttgaa aaaattgtac atcaaagggt
    58081 agagagtgga gaaccactta ctgctgatga atttactaat atatactaca aattaaatga
    58141 aaagtattac ggaaaatctg caatagtaga taaacaaata ggaatagaat gggcgagaat
    58201 acctcatttt tattcaaact tctatgtata taagtatgcc acagggtttt ctgctgcaag
    58261 tgctttaagt caacagatac tttcagaagg aagtactgca gtagatagat atataaactt
    58321 cttaaaaagt ggagggtcag aatacccact agaacaatta aaatctgctg gtgtagatat
    58381 gacgaaaaaa gaatctatag aggaagcctt aaatgtattt gctgaattag ttaataaact
    58441 agaaaaagaa ttataggatg tgataggttg atagcttcaa tgaagacttc taataatatg
    58501 agttgtatta tgtttatatc tgcctaaagt aactttaaaa agttaatcta attaagaata
    58561 aatcatataa aattaagttt ttacatgtat ttaacaatat ttacatttac ttaaccctgc
    58621 tctaatattc atttaacaaa gaatctgtat tattaaaata tagaaaaatg acaacaaaaa
    58681 attaaaatat aagcgggggt aagtaaaatg tttaaaaaga aaataggcgt tcttttagct
    58741 agtacaatgt tagttggagc tttagcagta ggatgtagtt ctaactcagg aggttcaggt
    58801 gattcaggag attcaagcaa agtttctgtt tcaggttcaa cttcaatagg tcctcttatg
    58861 gaaactatcg gagaaaagtt tcaagaaaaa aatcaaggtg ttagtgtaga agtacaacag
    58921 attggttctt ctggaggtat aaaaaatgct atagaaggaa catctgaaat aggaatgagt
    58981 tcaagagatt taaaagatga agaaaaaagt gctggattaa aggaaactaa aatagcaata
    59041 gatggtatag cattgataac taatactgca aactcagtaa aaaatctgac aatggaccaa
    59101 gttaaaggta tatatacagg aaagattact aactggaaag aagtaggagg aaatgatgca
    59161 ccaatagtaa tagtatcaag agaagaaggt tcaggaacta gagatggatt ccaagaaatt
    59221 gtagggtttg attcagaaga actaactaaa gatgcacaaa taagtgatgg ttcaggaaat
    59281 ataaaaacta cagttgaagg aaatgaaaat gctataggat atatatcatt tggatatgtt
    59341 gatgagaaag taaatagttt aaaaataaat gatgttgaag caactgatgc aaatgtaata
    59401 gctaataaat atccaatatc aagaccattt atattagtta ataaagaaga tggtttgtca
    59461 gaaaaaggta aagctttaat agattatata ttaagtgatg aaggacaagc acttgtttca
    59521 gaaaaaggat ttattaaagt taatcaataa caagagttat aatataaagt aaacatataa
    59581 taccctgtga gataagctca taaaaatgat gctttaactt gcagggtatt gtctattatt
    59641 gaatgtagaa atataaatat ctttagataa aataggtatt ttagtatctt atatctaaga
    59701 atatggaaat attgacaaaa taatattaca atgttagaat aaaaaagaaa tttatgaaaa
    59761 tatataaaag atttttaata aaaatatttt agaaactgag aagtatatct tattattggg
    59821 catctggaga tatatggagt tagtggtgca accggctatg aatataattt ttatattgca
    59881 tagtcttttt ttgttttaaa attaacaaaa atgttgcaaa atttgaaaat aagagtatta
    59941 gtcgttaaga tttttattga taggtaaaat tttggctttt aaagtagcca tttgttggaa
    60001 aagggaaatt tttttaaaag aataatagta ttataataag aaaataaagt ttttataaac
    60061 attgagatgc agaatataaa ccttttgtaa caatatattt accaaaataa tacaatttat
    60121 tttatattat aaccatataa atcatataaa tcaatactca aactagccaa tagacaagtt
    60181 tctagaaaaa taataaattt atacgtgaaa atgactttaa gctccatt
//
LOCUS       DAGSRM010000013        60213 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.013, whole genome shotgun sequence.
ACCESSION   DAGSRM010000013 DAGSRM010000000
VERSION     DAGSRM010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 60213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60213
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.013"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(61..852)
                     /gene="bclA2"
                     /locus_tag="MJE33_000912"
                     /pseudo
     CDS             complement(61..852)
                     /gene="bclA2"
                     /locus_tag="MJE33_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
     gene            complement(939..1340)
                     /locus_tag="MJE33_000913"
     CDS             complement(939..1340)
                     /locus_tag="MJE33_000913"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646880.1"
                     /translation="MPFAVFASAKPHGTRAFARLFVHAGGRFPRPLAGAIGTLFENAK
                     LIFPPGAVETASAAIFIISGTTGLIAVKSRLLLHEQFKRPLLALCEIHSGCASFPAGI
                     KKRHNFFEAAAAYPTHLKTPYQGLSNLATAF"
     gene            1412..2356
                     /locus_tag="MJE33_000914"
     CDS             1412..2356
                     /locus_tag="MJE33_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003505437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY9646881.1"
                     /translation="MAVFRVERNTGYTVMSNHHLRNKELSLKAKGLLSQMLSLPEDWD
                     YTLAGLSHINREKIDAIREAVKELEKAGYIVRSRERDEKGRLRGADYVIYEQPQPREP
                     EAATSGGQPPILDLPTLENPTLDNPTLEKPTQEKPTLENPTQLNKDISSKEQSITDLS
                     STHSFPFHSLNPLPFEQGEAATPPERKRTEAKSNSAVEIYREIIKDNIEYDHLIQNCK
                     IDADRLNEIVDLMLETVCTARKTIRIAGDDYPAELVKSKFLKLNSSHIEFVLDCMREN
                     TTKVRNIKQYLKAVLFNAPSTIDSYYTALVNHDFYGSK"
     gene            2393..2866
                     /locus_tag="MJE33_000915"
     CDS             2393..2866
                     /locus_tag="MJE33_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003505435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HBY9646882.1"
                     /translation="MQEEVTQKTIALSVNVGKGAARLTEQALQKAIKKFLEQKSKAPH
                     GKQTMRQLMKQNAGVSNIEITDSNIKAFESTAKKYNIDFSLKKVKGEQTRYLVFFKGR
                     DADVMIAAFQEFSAKKLNRDKKPSIRKALAAAKDKAKQLNAARDKVKKIDRGREI"
     gene            2863..3669
                     /locus_tag="MJE33_000916"
     CDS             2863..3669
                     /locus_tag="MJE33_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005335939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBY9646883.1"
                     /translation="MKQINYKKLIIPNIPYVFFVYLFDKVGQATRLAPGADISEKILN
                     ITQGFSEAFSNALPSVHPLDLLIGIVGAVVIRLIVYVKGKNAKKYRKGAEYGSARWGN
                     AEDIKPYIDPDFQNNIILTQTERLTMNSRPKQPKYARNKNVVVIGGSGSGKTRFFVKP
                     NLMQLHSSYVLTDPKGTVLIECGKLLQRAGYRIKVLNTINFRKSMHYNPFVYIRSEKD
                     VLKVVNTLIVNTKGEGEKSAEDFWVSATRSQAVKSLRTGNGFPLFGELIV"
     gene            4294..6201
                     /gene="ltrA"
                     /locus_tag="MJE33_000917"
     CDS             4294..6201
                     /gene="ltrA"
                     /locus_tag="MJE33_000917"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004853597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBY9646884.1"
                     /translation="MPKKSKNLCVDDLRHAEYYGMQPVFDKLYRRSLDGDNFTDLMDI
                     ILSRDNILLAYRNIKANGGSYTAGTDNKNISDIGCLPPETVVEKVRFIVTGSQHGYRP
                     KPVRRKDIPKPNGKTRPLGIPCIWDRLVQQCIKQVMEPICEAKFSDNSYGFRPNRSVE
                     HAMQRTYTMLQRMNLHYVIEFDIKGFFDNVNHSKLVRQIWALGIHDKQLIFIIKRILK
                     APIRMPDGTTLIPDKGTPQGGIISPLLANIVLNELDNWVESQWQNHPLVKEYGYERKI
                     RNSITFDRSKAFLKMRKTGLKEMYIVRYADDFRIFCRNKEDALRTKEAVTAWITERLR
                     LEVSPEKTRIVNVRKRYSEFLGFKIRVRPKSGRYAVQSHICDKKLELEKQKLVEQAKR
                     IARPSKGKRPLDEIRLFNSMVLGIQNYFQLATCISIDCRKIHRQVMTVLTNRLNTETG
                     CKLVREGGAMTESEKERFGKSAMVRYVSGIDQPIYPIAYIKNKIPMAKKTAVCSYTAD
                     GRALIHTNLSLNSFVLAGLRNQPSMGRSTELTDSRISLFSAQRGKCALSGELFENAAD
                     IVCWLKTPAEFGGRERYQNMILFHRRFLPLLQRRPMNELKAIANALKTTKELMLKVNS
                     LRERAGLSAIE"
     gene            6316..7389
                     /locus_tag="MJE33_000918"
                     /pseudo
     CDS             6316..7389
                     /locus_tag="MJE33_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
     gene            7453..7668
                     /locus_tag="MJE33_000919"
     CDS             7453..7668
                     /locus_tag="MJE33_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family protein"
                     /protein_id="HBY9646885.1"
                     /translation="MEFFNSAVGVLQTLVIALGAGLGIWGAINLMEGYGNDNPGAKSQ
                     GMKQLMAGGGVALIGGTLVPLLSQLFG"
     gene            7751..8602
                     /locus_tag="MJE33_000920"
     CDS             7751..8602
                     /locus_tag="MJE33_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646886.1"
                     /translation="MIEDWFRGILTDGILSNLSGLFDSVNTEVGEIATQVGTTPAGWN
                     AGIFNMIRSLSENVIVPIAGVIITFVMCYELIQLVIEKNNLHDLDTWIFFKWIFKTFV
                     AVLLVTNTWNIVMGVFDVTQSVVNQSAGVIISDTSIDVTTVITDIEAKLDAMSVGGLL
                     GLWFQSLFVGLTMKALSICIMLVVYGRMIEIYLVTSVAPIPMATMVNHEWGSMGQNYL
                     KSLLALGFQAFLILVCVGIYAVLIQTIAATDDISGAIWACMGYTVLLCFTLFKTGSLA
                     KSVFSAH"
     gene            8619..9515
                     /locus_tag="MJE33_000921"
     CDS             8619..9515
                     /locus_tag="MJE33_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBY9646887.1"
                     /translation="MKIGLIDVDSHNFPNLCLMKLSAYHKAKGHTVEWWNAKGRYDLV
                     YKSRVFTDTYSKDTITVTNAEQVIFGGTGYDTKNRLLPEVEHSYPDYSIYPQFFGIAY
                     GFLSRGCPRNCGFCIVSSKEGRESVKVADLSEFWKWQPEIKIMDANLLACPDHENLIE
                     QLIRSRAWVDFSQGLDIRLVNRDNVSLLNRVRIKAVHFAWDNPDEDLTGYFQRFLDLT
                     AIKSSRQRRVYVLTNYGSTHEQDLYRVNTLRAMGFDPYVMIYERPTAPPVTRHLQRWV
                     NNKRLFYAVPRFEDYIPGRKEV"
     gene            9517..9909
                     /locus_tag="MJE33_000922"
     CDS             9517..9909
                     /locus_tag="MJE33_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HBY9646888.1"
                     /translation="MAYVTVPKDLTKIKSKVMFNLTKRQLICFSAAVAIGLPLFFLVK
                     DSVGTTTAALCMVLAMLPMFLLAMYEKNGQPLEVIIRQFVEVKFLRPKERPYQTNNFY
                     AALARQEQLDKEVRAIVKGKEKHPKRKA"
     gene            9869..12265
                     /locus_tag="MJE33_000923"
     CDS             9869..12265
                     /locus_tag="MJE33_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003502116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBY9646889.1"
                     /translation="MSKEKKNTQNEKRKLSRQERKQIDTLIRQAKGDGKPHTAQDSIP
                     FERMYKDGICRLANGRYSKCIEFEDINYQLAQPDDKTAIFEALCDMYNSFDASISVQL
                     SLISRHANKDDFKNSITIAPQNDDFDSIRAEYTEMLRTQLERGNNGLIKTKFLTFTVE
                     AKDIRSARARLARIETDTLNHFKVIGAAARVLDGKQRLEVLHGIFHPDGERFNFAWEW
                     LPASGLSVKDFIAPSSFRFGDGRMFQMGGKFGAVSFLQIVAPELSDRMLADFMDAENG
                     IIVNLHIQSIDHNEAIKTIKRKITDLDRMKIEEQKKAIRSGYDMDIIPSDLATYGGEA
                     KNLLRDLQSRNERMFLLTLLVLNLADTKQKLDNEVFGAAAIAQKYNCILTRLDYRQEQ
                     GLMSSIPLGENLIHIQRGLTTSSTAVFVPFTVQELFQSGEALYYGLNALSNNMILCDR
                     KKLKNPNGLILGTPGSGKSFSAKREITNAFLITSDDIIICDPEAEYYPLVGRLKGQVI
                     KVSQNSTQYINPMDINLNYSEGDTPIALKSDFILSFCELIMGGKNGLEAVEKTLIDRA
                     VISVYRNYLADPKPENMPILGDLYDEIKKQPEKEAQRIAAALELYVNGSLNIFNHRTN
                     VDIHNRLVCFDIKELGTQLKKVGMLIVQDQVWNRVTVNRSEGKATRYYIDEFHLLLKE
                     EQTAAYSVEIWKRFRKWGGIPTGITQNVKDLLTSREVSNVFENSDFVYMLNQAQGDRE
                     ILAKQLNISQQQMTYVTHSEAGEGLIFYGNVILPFIDRFPKDTELYRVMTTKPGEVSA
                     "
     gene            12262..13218
                     /locus_tag="MJE33_000924"
     CDS             12262..13218
                     /locus_tag="MJE33_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006774458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HBY9646890.1"
                     /translation="MRIETDKIYCGDSLQVLQTLPDNCLDCCVTSPPYYALRDYGTDG
                     QIGREATPEEYVSRITAVFHEVKRVLTPEGTCWLNIADTYCGTGSKADHQDPKYPKGR
                     NGQQVAVNHRAPGCKPKDLIGIPWLVALALRGDGWYLRSSIIWHKGNAMPESTRDRPT
                     RCYEYVFLLTKSKKYYYDWQAVAEPIAPTTAVRLKSGVGKGNKYAATVPGQNQPQKIN
                     RPRRKGAYTDEMISPVRSRRNVWQINTTSYRGGHFAAFPPKLAETCILAGCPVGGIVL
                     DPFLGSGTTAAAAKSLSRRYIGIEINPEYCTLAKQRIGGDEH"
     gene            13215..15185
                     /locus_tag="MJE33_000925"
     CDS             13215..15185
                     /locus_tag="MJE33_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBY9646891.1"
                     /translation="MSKELKAPDKVTQKMTRDGAIAENLTTGETTSISERPPEENYSA
                     PAAGAAEKATQRISTEVSRHLEKGAEKKSLDAAQLKTHTSRLQFSAEERATPELQKAI
                     RKSDKAADRLDAARAAIPKHTKIKKERVFNEAKGRAKTRLSFEKTDKPPNGKLRHNPL
                     SRPAQELNSTVHGKIYEVEKENVGVESGHRVELAGEKAGGMAARTVKRGIRSHKLKPY
                     RAAAAAERKAVKANADFLYQKALHDNPALAHSNPISRFWQKQRIKKQYAKTLKAGGKV
                     KKTAEATAKAAKKTAQETKRATFFVVRHWKGCLLVGGIAFIVLLLFGGLSSCSLFGGN
                     SGSGLIASSYLSEDADITGAEAAYTDMEAELQDMLNNIEREYPGYDEYRVNADEIEHD
                     PYVLISILSALHEGVFTLDEAQSTLEMLFEKQYILTVTEEVEVRYRTETRTDSEGNEY
                     EVEVPYNYYILHVDLENFNLSHVPVYIMGEEQLSMYAMYMSSLGNRPDLFPSSGYVSK
                     YYENPPADYEVPAALLNSDEKFARLIEEADKYVGFPYVWGGSTPETSFDCSGFVSYVL
                     TNSGLYNTGRLGAQGLYNISTRVSNPQPGDLVFFTGTYDTPGVSHVGIYVGEDGDGSP
                     VMLHCGDPIQYTKLDTSYWQSHFYAYGRLHYN"
     gene            15207..15473
                     /locus_tag="MJE33_000926"
     CDS             15207..15473
                     /locus_tag="MJE33_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006774456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HBY9646892.1"
                     /translation="MANTKLDRIERDIEKTRAKILEYQKRLKDLEAQKIEEENAQIVQ
                     MVKAVHLDGAQLAAFLSAYASGEIALPQPDADYTDEQEETEDEA"
     gene            15463..16194
                     /locus_tag="MJE33_000927"
     CDS             15463..16194
                     /locus_tag="MJE33_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008979753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HBY9646893.1"
                     /translation="MKHKKLFRSVTALLAALVLMGGFSVTAFAGGGDVSDEPPTPVTE
                     TEPEETTGGYEPQPLTPEGNMSLVDDITGEASGDKQFITVVTKNGNYFYIIIDRAEDG
                     ENTVHFLNQVDEKDLLQLMEDEQTETPACSCTEKCVAGSINTDCPVCSVNMSECAGKE
                     AEPELEETEQPEPEEDKGGGMGGAILLVVLLLAAAGGGAFYYFKIMKPKKDAAKGSSN
                     LDDLDFDEDDEDAEELETEQEDEEV"
     gene            16191..18266
                     /locus_tag="MJE33_000928"
     CDS             16191..18266
                     /locus_tag="MJE33_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HBY9646894.1"
                     /translation="MKLVIAEKPSVGAAIAAVLCANEKRSGYFEGSGYLVSWCIGHLI
                     SLADAATYNEQYRKWKYDDLPIVPQDWQFIVASGKEQQFSVLKDLMHRSDVSEIVNAC
                     DSGREGELIFRFVYEQANCKKPFSRLWISSMEESAIREGFSNLKDGRSYDNLYQSALC
                     RAKADWLVGINATRLFSILYHKTLNVGRVQTPTLTMLVNRDYAISSFKKEKYHVVRLD
                     AGGVSALSERLNDEAAAQQMKAACEKSQAVCTSLKKEKKTVAPPKLFDLTALQREANR
                     LYGFTAKQTLDYAQALYEKRLLTYPRTDSKYITSDMQDSTKELITGLCSLLPFMRDVK
                     LQADLIRVCDNSKVTDHHAILPTAEFLKAGFASLADSETKLMTLVCAKLLCAAAAPYA
                     YEAVTAVFTCGGYTFTAKGRTTLCEGWREIERLSRAASEEQDEDAEPEAVLPPLTEGQ
                     TFENTAAEISERYTQPPKAYTEDTLLSAMENAGKEETPEDAERKGLGTTATRAGIIEK
                     LISAGFAERKGKKLIPTKDGYNLVAILPESLTSPQLTAEWETRLTGIAKGSDSPADFM
                     RGIEEMTAGLVKTYSAISEDKAKLFTPQREAIGTCPRCGAAVYEGKKNYYCSDRACSF
                     VMWKNDLFFQQRKKTFTKAIAAALLKDGKVKIKGMYSTKTGKTFDGVVLLADTGGKYV
                     NFRVEQNRK"
     gene            18263..18631
                     /locus_tag="MJE33_000929"
     CDS             18263..18631
                     /locus_tag="MJE33_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HBY9646895.1"
                     /translation="MIRLDEKQYRAVKKMARAACANNDCGNCLLLDDGETCVCVQSIS
                     YSLLCRYFREAVLPADRQLCEQITRSGETDLKRCAVCGSTFAAGSNRAKYCPDCAAKI
                     RRRQKAESERNRRLRIKITT"
     gene            18757..22155
                     /locus_tag="MJE33_000930"
     CDS             18757..22155
                     /locus_tag="MJE33_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015574397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBY9646896.1"
                     /translation="MPYSSYDHDKLETAETMRIERRIYFEAKDGDIAPYVSLPLEQLH
                     AMREESAAAEQAIFNDLSTRAAAWEQQAGKTLLLDKAIEYTRTTVVQHTSNEWQKGEY
                     DRYTRSNRVYQMNYHIYENTRYDREKQQSVPYSYSLTWGVYTNSPNRNGQAKIAGQDR
                     KVFSDKAAMEKYLNGRIKAYDRLFTEISPPIPQEYAEHFKVNGMLLPGYTIEGEEPPQ
                     QQPQAAAIPETTGQQKEREHMSEQFSIMIGNRSRFEAGDPGGYWLDMPATKEQLQTAM
                     QSVGITADNPQDFSIRGYSDDPEKHIALPYEMVCAADVDELNFLAARLEQLDPAEVGK
                     LNAALQQKNGLANIGQVIDFTYNVDFYVHIPEVHTYRDLGDYYLNQSGMVQMPEEWKG
                     GIDLTTFGRNAAAQEKGAFTEYGYIVESGDEWERQFEGREVPEEYRIMSYPQPERGEQ
                     DKAYMDAAETQQAAVQTAEPQQPRPVVPIILSSEKPGDKVKEITARLEQGIQAIFDSD
                     RYKEFLTAMSKFHDYSLNNTILIAMQGGNLVKGYSQWQKEFDRHVKSGEKGIKIFAPA
                     PYKVKKLVDKIDPDTRKPILDSEGKTVKEEKEVTVPAFKVVTVFDISQTEGKEFPDLS
                     VKPLLADVEQYEDFFAALEKASPVPIAFEQITNGANGYFSLTDKRIAIKEGVSELQAV
                     KTAIHEIAHAKLHDVDLNAPLEEQNRVDRRSREVQAESVAYTVCQHFGLDTSDYSFGY
                     VAGWSSGKEMTELKASLETIQATAKERITEIEGHFTELQQQRQAEQEQEAKTKETLLL
                     NGKEDSYGIYQLARREETMDLRFEPFDRLQATGHFVDRANYELVYTAPLTKDMTLGGI
                     WEKFNIDRPADFKGHSLSVSDIVVLHQNGENTAHYVDSIGFQQVPEFLQEQAQQTPVF
                     DKLSPEQQQALSDTVKDTLQLLVDADKRIYGDVTGKTLEAIAAQGYSYKGGQLEKQQP
                     EATPESLMTGETVRTPRGNFYITDMSREQIEAAGFGFHHASEDGKYLIMGNGTQAYAI
                     AAEQPQRDNPLKHVEDIVEQNDNNFDGIINNTPQTPTVADLEQRAKAGEAISVTDLAK
                     AVKAEKREQPQKKPSILKKLDEYKKQAAQQPKDKQKEQKKDLEV"
     gene            22160..22369
                     /locus_tag="MJE33_000931"
     CDS             22160..22369
                     /locus_tag="MJE33_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HBY9646897.1"
                     /translation="MNIRFTIEEENIIAIYAEDSRETTLENILAAMPYMDEDMRQLAA
                     AAVKKLQAMTDSEFSEREFILTDEE"
     gene            complement(22571..23905)
                     /locus_tag="MJE33_000932"
     CDS             complement(22571..23905)
                     /locus_tag="MJE33_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003508281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBY9646898.1"
                     /translation="MAVTKTHPIKSTLKAAIDYILNPEKTDGKLLASSFGCGLETADI
                     EFAWTREAAGDRGTHLGRHLIQSFAVGETTPEEAHKIGMELAQAVLGGKYEFVLTTHV
                     DKDHLHNHLIFNAVSFVDYKKYHSNKQSYHAIRRTSDRICKEHGLSVVVPGQDKGKSY
                     AEYTAEKQGTSYKAKLKTAIDTLIPQVKDFDELLRRLQEMGYEIKQGKYISFRAAGQE
                     RFTRAKTLGAAYTEEAIKERIKGVYIAKSKPLREDKKIRLVVDIENSIKAQQSAGYER
                     WAKIHNLKQAAKSMNFLTENKIEYYSDLESKIADIMTAHDAAARAVKEVEQRMSDLSL
                     LIKHTTTYRQLKPIYEEYKKSRDKEKYLRGHESEIILFEAAARALREMQIKKLPDLAA
                     LRKEYSGLNDRKSKLYEDYRQAKKQMQEYGVVKKNVDSILYPSQSKAREQER"
     gene            complement(23866..24195)
                     /gene="mobC"
                     /locus_tag="MJE33_000933"
     CDS             complement(23866..24195)
                     /gene="mobC"
                     /locus_tag="MJE33_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBY9646899.1"
                     /translation="MENRKRNIQMKFYVTEEEKRLIDEKMKQLPIKQYGAYFRKMAID
                     GYILVIDRSDTKAYIRELQAVSRNINQIAKRANATGAVYQQDIEDIKKAVDEIWRLQR
                     RTLLNQP"
     gene            complement(24397..24753)
                     /locus_tag="MJE33_000934"
     CDS             complement(24397..24753)
                     /locus_tag="MJE33_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY9646900.1"
                     /translation="MAKRPVPLYDFKAFGAAIKAARNEYGESRKKVSDELYISPRYLA
                     NIENKGQQPSLQVFYDLVTRYHISVDQFFFPNSNAEKSTGRRQLDALLDGMSDKGIRI
                     VTATAREITEVEKAED"
     gene            25140..25325
                     /locus_tag="MJE33_000935"
     CDS             25140..25325
                     /locus_tag="MJE33_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBY9646901.1"
                     /translation="MLKKYWIKCPICNGKTRVQVFHNTVLKDFPLFCPKCKLTHIIDV
                     EKLEIVIKNTEKQTFII"
     gene            25342..26328
                     /locus_tag="MJE33_000936"
     CDS             25342..26328
                     /locus_tag="MJE33_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006774449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBY9646902.1"
                     /translation="MKRLPKYTPAEVRNDPYGFTYKEMSEVIGENEAKALYEELYKQL
                     PRKKNLSMLVKNICKSSDTEKYVYELKDNKYIETVFIKRRDGGTVCVSTQVGCPVGCI
                     FCESGRNGFVRNLTSSEIVQQIILLRRKVNRIVFMGMGEPLFNYDNLIKAIHILRDRY
                     GLNFPTDGITISTVGPVDQLKKLREEHLKIQLTISLHAATQSARNRIIPHMRIYAIED
                     VVKQALSYSERHNRKIVFAYLLLPGINDRPSDVRQLAKWFRGKKVMINVLQYNPTSNS
                     RIKAPQKREIVAFKHQLEQAGLEVTMRVSHGREINAACGQLANTYNKFKKND"
     gene            26798..27235
                     /locus_tag="MJE33_000937"
     CDS             26798..27235
                     /locus_tag="MJE33_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003508282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBY9646903.1"
                     /translation="MELSSSDKERIQHQYDALAKKTLVGEAKSHRRTLAKRAAREVTF
                     SDLSESELAQLFTTDEYESDYFRFQVSGFDVLVKNELLAEALNALPERKRDIILLSYF
                     LDMSDAEIGELLNVVRTTVFRHRKSALAKIKQYLEGKADDEYR"
     gene            27219..27461
                     /locus_tag="MJE33_000938"
     CDS             27219..27461
                     /locus_tag="MJE33_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY9646904.1"
                     /translation="MMNTVRKSENLLPFPVISAAANGDTTAMCAILKHYEGYIAKLCT
                     RTLKDDAGNTYSYVDEEMRNRLQVRLITRTLAFHVG"
     gene            27942..28196
                     /locus_tag="MJE33_000939"
     CDS             27942..28196
                     /locus_tag="MJE33_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008979760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646905.1"
                     /translation="MITKLQLQQMRNVDITQVDRSTLVDIRNIHIDSSLPAAKKMQSY
                     LEQIVNPYCFLCGDTPVRIRFVAEDRTLKQSLCDYFLSLK"
     gene            28291..29973
                     /locus_tag="MJE33_000940"
     CDS             28291..29973
                     /locus_tag="MJE33_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBY9646906.1"
                     /translation="MPRIRKDKMARSYAEPFWKIGLYIRLSREDDNEDESESVINQEK
                     ILRDFVDSYFEPGTYVIVDVFADDGLTGTDTARPNFKRLEGCIVRKEVNCMIIKSLAR
                     GFRNLADQQKFLEEFIPINGARFICTGTPFIDTYANPHSASGLEVPIRGMFNEQFAAT
                     TSEEIRKTFKMKRERGEFIGAFAPYGYKKDPNDKNSLIVDEEAAEVVKSIYHWFVNEG
                     YSKMGIAKRLNQMGEPNPEAYKKKKGFKYNNPNSDKNDGLWSASTIVRILQNEVYTGV
                     MVQGRNRVISYKVHKQINVPEEEWFVVPNTHEAIIDRETFEKAQALHKRDTRTAPGKQ
                     EVYLMSGFVRCADCKKAMRRKTARDIAYYSCRSYTDKKICSKHSIRQDKLENAVLAAL
                     QMQIALVDRLAEEIERINNAPVINRESKRLSHSLKQAEKQLKQYNDASDSLYLDWKSG
                     EITKEEYRRLKGKIAEQIQQLEANISYLKEEMQVMADGIGTDDPYLTAFLKYKNIQSL
                     NRGIMVELVKAVWVHENGEITVDFNFADEYQRIIDYIENNHNVIRVIENKAI"
     gene            complement(30030..30923)
                     /gene="bclA2"
                     /locus_tag="MJE33_000941"
                     /pseudo
     CDS             complement(30030..30923)
                     /gene="bclA2"
                     /locus_tag="MJE33_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
     gene            complement(31301..31840)
                     /gene="hpt"
                     /locus_tag="MJE33_000942"
     CDS             complement(31301..31840)
                     /gene="hpt"
                     /locus_tag="MJE33_000942"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBY9646907.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLRELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRNINLPLRVNFMTTSSYGNNEESTGRVKVVSDVTTDIAGYDVLVVDDI
                     TDSALTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
     gene            complement(32168..33475)
                     /locus_tag="MJE33_000943"
     CDS             complement(32168..33475)
                     /locus_tag="MJE33_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBY9646908.1"
                     /translation="MRDIRKELVEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIV
                     ENRMLVSPSIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIA
                     EDYMDNTPLSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNE
                     PKVSASNDAAEKAESLMDTVTIQELIKNVEGIDFKDIEFLLDGVKMNEEMAEYGLKQK
                     TGIGVGYGIKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTA
                     ILPIVAYNKKFPQSDERLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGIS
                     WLMNGTEKQIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVP
                     PLNGIVGSSVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            complement(33900..35675)
                     /locus_tag="MJE33_000944"
     CDS             complement(33900..35675)
                     /locus_tag="MJE33_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY9646909.1"
                     /translation="MMIMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKELFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDICRECYKSGACVEFAEVCCPIICD
                     GDSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKIDKYNSKFKELFNIKDSITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLEKMVQNGTFREDLYYRLNVIPIKLP
                     SLRERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVKSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            complement(35672..35809)
                     /locus_tag="MJE33_000945"
     CDS             complement(35672..35809)
                     /locus_tag="MJE33_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646910.1"
                     /translation="MEINYELIIIVMLFVILMSIQFTLNKIYLILKEIRQILMLKKNK
                     E"
     gene            complement(35887..36456)
                     /locus_tag="MJE33_000946"
     CDS             complement(35887..36456)
                     /locus_tag="MJE33_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBY9646911.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            complement(36640..37275)
                     /locus_tag="MJE33_000947"
     CDS             complement(36640..37275)
                     /locus_tag="MJE33_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBY9646912.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREITDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
     gene            complement(37943..38824)
                     /locus_tag="MJE33_000948"
     CDS             complement(37943..38824)
                     /locus_tag="MJE33_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBY9646913.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWINVLP
                     GGGNELGLTGAKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
     gene            complement(38944..39951)
                     /locus_tag="MJE33_000949"
     CDS             complement(38944..39951)
                     /locus_tag="MJE33_000949"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HBY9646914.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            complement(39971..40453)
                     /locus_tag="MJE33_000950"
     CDS             complement(39971..40453)
                     /locus_tag="MJE33_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HBY9646915.1"
                     /translation="MSFVGISTSMKNTSLHIFRDPLLDLIDNDSDIDLLGVVVVGTSE
                     HNEWKTFLATRLGRWIESLRPDGVIITLDMAGNQHIDFTNAIAEFVKSDIPTVGLTIM
                     GADGLVITNPYLDKATIIDYKKTTGYIETEVVGDNHMDEVDVKKALAFLKLKMRKDAK
                     "
     gene            complement(40471..40938)
                     /locus_tag="MJE33_000951"
     CDS             complement(40471..40938)
                     /locus_tag="MJE33_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HBY9646916.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVESDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            complement(40953..41711)
                     /gene="prdD"
                     /locus_tag="MJE33_000952"
     CDS             complement(40953..41711)
                     /gene="prdD"
                     /locus_tag="MJE33_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HBY9646917.1"
                     /translation="MEEKIMRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKDQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKIRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            complement(41791..42516)
                     /gene="prdB"
                     /locus_tag="MJE33_000953"
     CDS             complement(41791..42516)
                     /gene="prdB"
                     /locus_tag="MJE33_000953"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(42064..42066),aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HBY9646918.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            complement(42554..42838)
                     /locus_tag="MJE33_000954"
     CDS             complement(42554..42838)
                     /locus_tag="MJE33_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646919.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            complement(42919..44799)
                     /gene="prdA"
                     /locus_tag="MJE33_000955"
     CDS             complement(42919..44799)
                     /gene="prdA"
                     /locus_tag="MJE33_000955"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HBY9646920.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSTGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            complement(45228..46988)
                     /gene="prdR"
                     /locus_tag="MJE33_000956"
     CDS             complement(45228..46988)
                     /gene="prdR"
                     /locus_tag="MJE33_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator
                     PrdR"
                     /protein_id="HBY9646921.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSDQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISEKVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKDVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKIDVSKI"
     gene            complement(47395..49503)
                     /locus_tag="MJE33_000957"
     CDS             complement(47395..49503)
                     /locus_tag="MJE33_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HBY9646922.1"
                     /translation="MKTKIKKSSIISLVIAFSMVFTAFTPIVSYADEVTSNDTILNGE
                     EQSNGQTPDVEKPSDGQVPDGEKPLDGQIPDGEKPSDGQIPDGEKPSDGQIPDGEKPS
                     DGQIPDGEKPSDGQMPDGEKPSDSQTPDVEKPSDGQMPDGEKPLDEQIPEEEKPLEEE
                     IVIEEMSLKQDIDKILDMTLSQINKIVYNFWEDEEDVKADEQSEINQVFTSEDSFISL
                     WYDKKAKVKNSCLLKEIDGSDRPYHDLRFDDITKTLTFDYVIKGLVGASSKDDKYVIE
                     GEDGEQTAFCYNNHLRPPSSNGKSPYLPAEDFNGQENQNEEAVKSILYAGSEFDGFGY
                     KQQFNLGEEENDIMTYSATQSAIWIILGQIDEQERLKQYQGSIDLCDKLIEIAKTEEE
                     RAEYQKRKEVAINTKAYLEALLKAGREELKPNDTGKPSLSNGLTKINFEKNEDGTYET
                     EAVALVGYSGVVKLKLPDGVTAYDVDGNIIGTGEVEISTQQKFKLKSVGKPDAKANIS
                     AVSYDYIFPKAIQYYKAVLDLGQKDHSSSLPSSKQNLLSYTIEKKNGQEVNFNIGLPT
                     DDVVNPPVPPTDDTNGHKPKPSPPIDDTVINPPVPPMDDTIINPPVPPTEDAVLNPPV
                     PPTEDAVLNPPVPPTEDTVLNPPVPSVSDTVEKTPELSRDDTIIKSPKTGDETQLMSY
                     VFISVIAICGLAYQCKIKRN"
     regulatory      complement(50085..50170)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(50573..51886)
                     /gene="prdC"
                     /locus_tag="MJE33_000958"
     CDS             complement(50573..51886)
                     /gene="prdC"
                     /locus_tag="MJE33_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HBY9646923.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQEDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     gene            complement(52275..53039)
                     /locus_tag="MJE33_000959"
     CDS             complement(52275..53039)
                     /locus_tag="MJE33_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBY9646924.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKSDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKKTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESQPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKTKN"
     gene            53330..53533
                     /locus_tag="MJE33_000960"
     CDS             53330..53533
                     /locus_tag="MJE33_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBY9646925.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
     gene            53724..54260
                     /locus_tag="MJE33_000961"
     CDS             53724..54260
                     /locus_tag="MJE33_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HBY9646926.1"
                     /translation="MFENSSEELAYHKLLILYILEKIKMDLTNSQITQVVLETEMMNY
                     FSLQQLLSQLMESKFLTTYKDSDREYYALTQRGVESLEYFFNRIPTSVTEKIDKYIDS
                     NKENLLADTQVKSSFVKQSDNEFIVNLRVIENQSNLIDLNLNVSSEKQAKLICNNWKN
                     NASYMYAEVIDLLIRDIH"
     gene            complement(54264..55130)
                     /locus_tag="MJE33_000962"
     CDS             complement(54264..55130)
                     /locus_tag="MJE33_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HBY9646927.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            55278..55523
                     /locus_tag="MJE33_000963"
     CDS             55278..55523
                     /locus_tag="MJE33_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBY9646928.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            complement(55637..56935)
                     /locus_tag="MJE33_000964"
     CDS             complement(55637..56935)
                     /locus_tag="MJE33_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBY9646929.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILRECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVARIIELI
                     KEKIEIMVKEGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVDFDDINIKKSDINSQIYDCTVMKETYRDLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLINTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKG
                     RKLTLLIGMLEDKDIDSVLEILLPHFNKVITTTPNNPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(57068..58099)
                     /locus_tag="MJE33_000965"
     CDS             complement(57068..58099)
                     /locus_tag="MJE33_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBY9646930.1"
                     /translation="MENICDMNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYI
                     FISDKFDDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNI
                     RLINLSLSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGFT
                     LDGDDSFWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEK
                     PGITISDLNDKLESLATRNHWDSCDFDECSRIVNFKLDLYDKENALFMKVADVVRDCL
                     DIEENNEKLFKGSLFNKEIGLVYDNCFNLLKKLEHRFDIKFNYMDISKYDLVSIYTLT
                     ELVERYKNL"
     gene            complement(59145..59855)
                     /gene="rgaR"
                     /locus_tag="MJE33_000966"
     CDS             complement(59145..59855)
                     /gene="rgaR"
                     /locus_tag="MJE33_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgaR"
                     /protein_id="HBY9646931.1"
                     /translation="MFRVVICDDEKIQRSILKEFTEKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNMITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
BASE COUNT        17510 a        12692 c        10930 g        19081 t
ORIGIN      
        1 ctatttattg gtatatgaaa tcatattgtt tattatttta taatcttttc atcatataaa
       61 ctattgtatt ctataaactg atacatatcc agctgcagaa gtacctgtcg tgcctgaacc
      121 aaatgtaaaa cttaaagtaa cagctgctgc tctagcatca actaaaaagc ttaaacttac
      181 acttacatct gatgaagtaa gtgtatttac tgcttttgca ataaagttat tttgagctac
      241 tccattaaag ttagtagtaa ttgacataaa tcctgcaagt gaaagtgtgg cacgtatacc
      301 gaaagataca ttgtatatcc ctccaactgc aagatttata gtactacttg ttggaaaagt
      361 tatattagtt ccatttataa atgatgaatt aaatgtcact aatgtattat tcactaaact
      421 tgaagaggac actgtaaatt gtgcattgtt tgttgctaac actcctgttg ctcctgtcgc
      481 tccggttgga cctgttgctc catttgctcc tgttgctccg gtcgcccctg ttgctcctgt
      541 cgctccattt gctccagttg ggcctgttgg tcctatcgct cctgctactc catttgctcc
      601 cgttgctcct actgctcctg ttgggcctgt tgctccattt gcccctgttg ctcctgtcgc
      661 tcctgttgct cctgctactc catttgctcc ggttggaccg gttggaccta ttggacctgc
      721 tactccattc gctccggttg ctcctactgc tcccgttggg cctgttgctc catttgcccc
      781 tgttgctcct gtcgctcctg ttggacctac tgctccatct gctcccgttg ggcctgttgc
      841 tcctactgct ccgtcaatat atgcacaaca cggaaattgc cgtaaccatg cggctttcca
      901 ctatcttaaa gacaattaaa cagcccctat gattacacct aaaacgccgt agcaaggttg
      961 gacaagcctt gatatggcgt ttttaagtgg gtaggatatg ccgccgcagc ttcaaagaag
     1021 ttgtggcgtt tttttatgcc cgcaggaaag gaggcgcagc cggaatgtat ttcacaaagg
     1081 gcaagcagcg ggcgtttgaa ctgctcatgc agcagaagcc gggatttgac cgctatcaat
     1141 ccggttgtgc cggagatgat gaagattgcg gcacttgccg tttctaccgc cccgggtgga
     1201 aatatgagtt ttgcgttttc aaagagtgtc cctattgccc cggcaagagg acgcggaaaa
     1261 cgcccgccag catggacaaa tagacgggca aaagcccttg tgccatgcgg ctttgcagac
     1321 gcgaaaacgg caaagggcat attgcaatac caaaaccgcc gaaaacggct ttctaattgt
     1381 ccacgcagac caagagagga agtgaggaaa tatggcagtt ttcagagtgg agcgaaatac
     1441 gggatatacc gttatgagca accaccactt gcgcaacaaa gaattgtcct taaaggcaaa
     1501 gggcttgttg tcgcaaatgc tgtctttgcc ggaggattgg gattataccc ttgcgggctt
     1561 atcccatatc aaccgggaga agatcgacgc aatccgcgaa gcggtaaagg aactcgaaaa
     1621 agccggatat atcgtgcgca gccgggagcg cgacgaaaag ggacgcttgc ggggcgcgga
     1681 ttacgtcata tacgagcagc cgcagccgcg agagccggaa gcagctacca gcggcggaca
     1741 gccgcctata ttggatttac ctacattgga aaatccaaca ttggataatc caacgttgga
     1801 aaaacctacg caggaaaaac ctacgttgga aaatccaacg caattaaata aagatatatc
     1861 aagtaaagaa caatcaatta cggatttatc aagtacccat tcctttccat tccattccct
     1921 aaaccccttg ccctttgagc agggcgaagc ggctacgccg ccggaaagga aaagaacgga
     1981 agcgaaaagc aatagcgcag tagagattta cagggagatt atcaaggaca atatcgaata
     2041 cgaccatctc attcaaaact gcaaaattga cgcagaccgc ctaaatgaga tagttgacct
     2101 tatgctggaa accgtctgca cagcgcgaaa gacaatccgt attgccgggg acgactaccc
     2161 cgccgaatta gtgaaatcaa agtttttgaa gctgaacagc agccatattg agtttgtttt
     2221 ggattgcatg agggagaaca caaccaaagt gcgcaatatc aagcagtatt tgaaagcggt
     2281 actgttcaac gcgccgagta ccattgacag ctattatacc gcccttgtca atcacgactt
     2341 ttacggcagc aaatgagcat agccgcagtt taccgggaaa ggagttgata ccttgcagga
     2401 ggaagtaacc caaaaaacga ttgccctatc cgtcaatgtg ggaaaaggcg cggcaaggct
     2461 taccgaacag gcgttgcaaa aagcaatcaa gaagtttttg gagcagaaaa gcaaagcccc
     2521 gcatgggaaa cagaccatgc ggcagcttat gaagcaaaat gcgggtgttt ccaacatcga
     2581 gatcaccgac agcaatatca aagcctttga gagtaccgcg aagaagtaca acatagattt
     2641 ttcgctgaaa aaggttaagg gcgaacagac ccgttacctt gtgtttttca aaggcaggga
     2701 cgcggacgtt atgatcgcag cgtttcaaga gttttccgca aagaagctga acagggataa
     2761 aaagccctct atccgcaaag cccttgccgc agcaaaggac aaggcgaagc agcttaacgc
     2821 cgcccgcgac aaggtaaaga aaatagacag ggggcgcgag atatgaagca gataaactac
     2881 aaaaagctga taatcccgaa tatcccctat gtgttctttg tctatctctt tgataaagta
     2941 ggacaggcga cgcggcttgc ccccggcgcg gatatttccg aaaagatact caatatcaca
     3001 cagggatttt ccgaagcgtt ttcaaatgcc ttgccgagcg ttcacccgtt ggatttgctt
     3061 atcggcattg tcggcgcggt ggttattcgt ctgattgtct atgtcaaagg gaaaaacgca
     3121 aagaaatacc gcaagggcgc agaatacggc agcgcacgtt ggggcaacgc cgaagatata
     3181 aagccctaca tcgacccgga ttttcagaac aacatcattt tgacgcagac cgaacgcctt
     3241 accatgaaca gccgcccgaa gcagccgaaa tatgcgagaa ataaaaacgt cgtggtgatc
     3301 ggcggcagcg gcagcggcaa gacaaggttt ttcgtaaagc ctaatttaat gcagcttcat
     3361 tcttcctacg tcttgaccga cccaaaaggc accgtcctaa tcgagtgcgg aaagctgctg
     3421 caacgggcag gctaccgcat taaggtactg aacacgataa actttcggaa aagtatgcac
     3481 tataacccct ttgtctatat ccgcagcgaa aaggacgtgt taaaggttgt caataccctt
     3541 attgtcaata ccaaaggcga gggagaaaaa agcgcggaag atttttgggt gagtgcgact
     3601 cgttcgcagg cggtaaaaag tctgcgcacg ggcaacggtt ttccgttgtt cggagaactg
     3661 atagtttgat aggtggaatg acggggtaac gccctgaaac gcctaccctt gatgggcgtg
     3721 cattagctgt aatggctttt gtacgaagcc ccatgacaaa ggctgagagt aaccgtaacc
     3781 actgccaaaa gtggtcgaaa gcggtctggc ggcagactgt gttacctata ggccgagggt
     3841 ctataagata cctatggtta gaatgttcgc tttggcgggc ggactgacga acctgcgaat
     3901 gtacaggtct aaaagaagac cacgcagaaa tgcgtgggtg cgttaaagcg cagtcggtgt
     3961 ggttcagtag aaatttgccc tacgaacgac catgctgtgt tacaggcaca ggcaagccga
     4021 caggtctata gcaggaacct aagggtatgt atgtacagat agaactatcg gaacgaggaa
     4081 aggcagcaga ttccgcaagg aataatctga cgaagaacaa taagcagacg aactgctgct
     4141 gaaaagcagt ggtcgaacct atgatgtgct tgcgttatgt gagctacgga ggaatggcca
     4201 caagtcggtt gagataaaca ggcattattc aatccaacat aaggattcga gtaagaccaa
     4261 aagggtcact ttcgtaaaag gaggtgatgc cttatgccaa agaaaagcaa aaatctatgt
     4321 gtagacgatt tacggcacgc cgagtattac ggtatgcagc cagtttttga caaactctat
     4381 cgccgaagtc tggacggaga caactttacc gaccttatgg atataatcct aagccgggat
     4441 aacattcttc tggcatatcg gaacatcaaa gcaaacggag gaagctacac agcaggaaca
     4501 gacaacaaaa acatcagtga catcgggtgt ttaccgcccg aaacagttgt ggagaaagtg
     4561 aggtttattg tcacaggaag tcagcacgga taccgaccaa aaccggtcag gcgtaaagac
     4621 attccgaagc caaacggcaa gacccgcccg ttgggtattc cctgtatctg ggacaggctg
     4681 gtacagcaat gtatcaagca ggtcatggag cctatctgcg aagcgaaatt cagcgacaac
     4741 agctacggct ttcgccctaa ccgctccgta gaacacgcca tgcagagaac ctataccatg
     4801 ctccagcgaa tgaatctgca ttatgtgatt gagtttgata taaagggctt tttcgataat
     4861 gtaaatcaca gtaagctggt gcggcagata tgggctttgg gaatacacga caaacagttg
     4921 atttttatta tcaagcggat tctgaaagca ccgattagaa tgccagacgg cactaccctc
     4981 attccagaca aaggcacacc gcaaggtggt atcatttcac cgctacttgc gaatatcgtg
     5041 ctgaatgaac tggataactg ggtagagagc caatggcaga accaccctct tgtaaaggaa
     5101 tacgggtatg agaggaaaat cagaaactcg attacttttg accggagcaa ggcatttctc
     5161 aaaatgagga aaacgggact gaaagaaatg tacatcgtga gatatgcaga tgatttcaga
     5221 atcttctgtc ggaataaaga ggacgctttg agaacaaaag aagctgtaac agcgtggata
     5281 actgagaggc taaggcttga agtatcgccg gagaaaacaa ggatagtcaa tgtcagaaaa
     5341 cgctattcag agtttctcgg atttaagata cgggtcagac caaaaagcgg taggtacgct
     5401 gtgcaatctc atatctgtga caaaaagttg gaactggaaa agcaaaagct ggtggaacaa
     5461 gcgaagcgga ttgccagacc gtccaaagga aaaagacctt tagacgagat aaggctgttc
     5521 aattcaatgg ttctgggaat acagaattat tttcagctcg ctacctgtat cagtattgat
     5581 tgcagaaaaa tccacagaca ggtcatgaca gttttaacca accggctcaa tacagaaacc
     5641 gggtgcaaac ttgttcgaga aggcggtgca atgaccgaga gcgaaaagga gcggttcggt
     5701 aagtcggcaa tggtgcggta tgtatcagga atcgaccaac ctatctatcc tatcgcatat
     5761 atcaagaaca agataccgat ggcaaagaaa acagcggtat gcagttatac ggccgacgga
     5821 cgggcattga tacacacaaa cctaagcctg aactcatttg ttctggcggg tttgagaaat
     5881 caaccgtcta tgggtcgaag cacagaactc acagacagca ggatttcatt attctctgct
     5941 caacgtggaa aatgtgcgct aagtggagaa ctctttgaaa atgcggctga catcgtatgc
     6001 tggctgaaaa ctccggcaga atttggcggc agggaacgct atcaaaacat gattttgttc
     6061 caccggagat tccttccact tctgcaaaga cgcccgatga atgagctgaa agcgatagca
     6121 aatgctctca aaacaacaaa ggaactgatg ttaaaagtga acagtctgcg tgagcgggct
     6181 ggtttgtccg caatagaata atagtatttc atttggagat tgattaaatg cttgtgaaaa
     6241 caatcgatgg aacgccggat gcggtgaaag tcgcacgtcc ggtgtgaagc gggggaaaat
     6301 ccggcgataa cctcaaagga ttacctatca ctataagccg agagattatt atattgcgcg
     6361 ttgatcggct acatttggta tgaagccccc gccgaggaaa tgagctttac aacgctgctt
     6421 gaacttatca atgcaagcga agcacgcgag gacgacgagg aataccaaag ccccgttgat
     6481 ctgctctttg ccgatttaga agaacgcagc cccgaccatt tcgcggtgaa gcaatataaa
     6541 aaatacaaat tggcggcggg caaaacggca aagtcaatcc ttatttcttg cggtgcgcgt
     6601 cttgcccctt ttgatataga ggaactccgg gaactcatgt cctatgatga attggaactt
     6661 gatacattgg gcgacaggaa aaccgcgctt ttcctcataa tgagcgatac cgacgacacc
     6721 tttaattttg ttatcgctat tctgcaatcg cagcttttca atttgctttg tgataaagcg
     6781 gacgacgaat acaacggcaa attgcctatt catgtccggt ttttgcttga tgaatttgcc
     6841 aacatcgggc agataccccg ctttgataag ttaattgcga ccattcgcag cagggaaatg
     6901 tccgcttcta tcattctgca atcgcagagc caactaaaag ccatttacaa ggacgcggca
     6961 gaaatcatac ttgataatgc cgacagcacc ttgtttttgg gaggacgtgg gaaaaatgca
     7021 aaggatattt cggataactt gggacgggaa acaatcgaca gtttcaatac ttccgaaaat
     7081 cgcggcacgc aggtttctca tggacttact tatcaaaaat taggaaagga gctgatgaca
     7141 caggacgaaa tagcagttat ggacggtggg aaatgtattt tgcagctacg cggtgtgcgt
     7201 ccctttttaa gcgacaaata cgacatcacg aaacacccga actacaaata cctttccgac
     7261 ttcgacaaaa gaaacgcttt tgacgtggaa cggtatatgt ctacccgtcc ggcaatcgta
     7321 aagcccgatg aagcctttga catatacgaa atagatttgt ccgacgagga cgcagccgcc
     7381 gaggaataac ggcggcattt tcattttcaa caactttttt tgagccgcac agcggcagaa
     7441 agcgaggttt atatggagtt tttcaacagc gcagttggtg tattgcagac tttggttatc
     7501 gcgcttggcg caggtcttgg catttggggt gccattaacc tcatggaggg ttacggcaac
     7561 gacaatccgg gcgcaaagag ccagggcatg aagcagctca tggcgggcgg cggcgtcgct
     7621 ttgatcggcg gcacccttgt tcccctgctt tctcaacttt ttggataatg cctaaaacaa
     7681 ataccacccg ccgcgccctc tcggaaacgg gagggcgcgg gaaaggaggg attaactttt
     7741 gatatggcag atgattgaag attggtttcg cggcattttg acagacggga ttttatcgaa
     7801 cctttccggc ttgtttgaca gcgtaaatac agaggttgga gaaatcgcaa cacaagtcgg
     7861 cacgaccccc gccgggtgga acgcgggcat attcaacatg atacgcagcc tttcggaaaa
     7921 cgtcattgta ccgattgcgg gcgttatcat tacctttgtc atgtgttatg aactaatcca
     7981 gcttgtaatt gaaaaaaaca atctgcacga tctcgacact tggattttct tcaaatggat
     8041 ttttaagacc tttgttgcgg tgctgctggt aacaaacact tggaacatcg tcatgggagt
     8101 tttcgacgtt acgcagagcg tcgtcaatca gagcgcgggg gttatcatat ccgatacaag
     8161 catagacgtt accaccgtca ttaccgacat tgaagcaaaa ctcgacgcta tgagcgtggg
     8221 cggtcttttg gggttatggt ttcaatccct ctttgtaggg cttaccatga aagccctgtc
     8281 aatctgcatt atgcttgtgg tgtatgggcg catgattgaa atatatcttg taacgagcgt
     8341 tgcccctatc cctatggcaa caatggtaaa tcacgaatgg ggcagcatgg gacagaatta
     8401 cttaaaatct ttgcttgcgc ttggttttca ggcgtttctg atcttggtgt gtgtcggcat
     8461 ttatgcggtg ttgatacaaa caatcgcagc gaccgacgac atttccggcg cgatctgggc
     8521 ttgcatgggc tataccgtcc tcttgtgctt tacgcttttc aaaacgggaa gccttgcaaa
     8581 gagcgtcttt tcggcacatt aagggggtga gttatgattt gaaaatcggt ttaatcgacg
     8641 ttgacagcca taatttcccc aacctctgct tgatgaagct atccgcgtat cataaagcaa
     8701 aggggcatac ggtggaatgg tggaacgcaa aggggcggta cgaccttgta tataaaagcc
     8761 gcgtctttac ggacacctat tccaaagaca cgataaccgt taccaacgcg gaacaagtta
     8821 ttttcggcgg tacgggctac gacacgaaaa accgcttgct gccggaagtg gaacacagtt
     8881 acccggatta ttctatctac ccgcagtttt tcgggattgc ctacggcttt ttaagccggg
     8941 gttgtccgag aaattgcggg ttttgcattg tcagcagtaa agagggacgc gaaagtgtta
     9001 aggttgcgga tttgtccgag ttttggaaat ggcagccgga aataaaaatc atggacgcta
     9061 atctgcttgc ctgtcccgac catgaaaatc tcattgagca gcttatacga agccgcgcat
     9121 gggtggattt ttcgcaaggg ctggatattc gccttgtgaa cagggataat gtcagcctgt
     9181 taaatcgggt acggataaaa gcggtacatt ttgcatggga taatcccgac gaggacttaa
     9241 ccgggtattt ccaacgtttt ttggatttga ccgccattaa gagcagccgc cagcggcgcg
     9301 tgtatgttct taccaactac ggcagcactc acgaacagga tttataccgc gtgaacacct
     9361 tgcgggctat gggctttgac ccgtatgtga tgatttacga gcgtccaacc gccccgcctg
     9421 ttacccgcca tttgcaacgg tgggtaaata acaaacggct tttctatgct gtcccccgtt
     9481 ttgaagatta cattccgggc agaaaggagg tttgaaatgg cgtatgtaac cgtcccaaag
     9541 gacttaacaa aaatcaaaag caaggtaatg ttcaacctta cgaaacgaca gctaatttgt
     9601 ttcagcgcgg cggtagcaat cggactgccg ctgttctttt tggtaaaaga cagcgttgga
     9661 acaaccacag cggcattgtg catggtgctt gccatgctgc ctatgttttt gcttgccatg
     9721 tacgagaaaa acgggcaacc gctggaagtg attattcggc aatttgtgga agtgaagttt
     9781 cttcgcccca aagagcgacc ttatcagacc aacaattttt atgccgccct tgcgcggcaa
     9841 gagcaattag ataaggaggt acgggcgatt gtcaaaggaa aagaaaaaca cccaaaacga
     9901 aaagcgtaaa ctttcccgac aggaaagaaa acagatagat acccttatcc gacaggcaaa
     9961 gggcgacggg aagccccata cggcgcagga cagcataccg tttgagcgaa tgtataagga
    10021 cgggatttgc cgccttgcaa acgggcggta ttccaagtgc attgagtttg aggacatcaa
    10081 ctatcagctt gcgcagccgg acgacaaaac cgccattttt gaagcccttt gcgatatgta
    10141 taactcgttc gacgcttcta tcagcgtgca gctttcccta atcagccgcc atgcgaacaa
    10201 ggacgatttc aagaacagta tcacgattgc gccgcagaat gatgacttcg acagtatcag
    10261 agcagaatac accgaaatgc ttaggacgca gcttgaacgc ggaaacaacg ggcttatcaa
    10321 gaccaagttt ctcaccttta ccgttgaagc aaaggacatt agatcggcgc gggcgcgtct
    10381 tgcccgcatt gaaacggaca ccctcaacca ttttaaggtg atcggcgcgg cggcgcgggt
    10441 attggacggg aaacagcgtc ttgaagtgct gcatgggatt ttccacccgg acggggaacg
    10501 ctttaacttt gcgtgggaat ggctacccgc aagcggtctt tctgtcaaag actttatcgc
    10561 gccgtcctct ttccgatttg gcgacgggcg aatgtttcaa atgggcggca agtttggcgc
    10621 agtttccttt ttgcagatcg tcgcgccgga actctccgac cgtatgcttg ccgacttcat
    10681 ggacgcggaa aacgggatta tcgtcaatct gcacattcag agtatcgacc acaacgaagc
    10741 aatcaagacg attaagcgca agattaccga ccttgaccgt atgaaaatcg aggaacagaa
    10801 aaaagcaatc cgcagcggct acgacatgga tattattccg tccgaccttg ccacctacgg
    10861 cggggaagca aaaaacctac tccgcgattt gcagagccgc aacgaaagaa tgtttctgct
    10921 tacgctgctt gtcttaaacc ttgcagatac caagcagaaa ttggataacg aagtatttgg
    10981 agcggcggca atcgcgcaga aatacaactg cattttgacc cgccttgact accggcagga
    11041 acaggggctt atgtcctcta tcccgttggg ggaaaacctt atccatatcc agcgcggctt
    11101 gacgaccagc agcaccgccg ttttcgttcc ctttacggtg caagagctgt tccaaagcgg
    11161 cgaagcattg tattacggct taaacgccct ttccaacaac atgattttgt gcgaccgaaa
    11221 gaagctgaaa aatcccaacg gcttgatact cggcacaccg ggcagcggca aaagtttttc
    11281 tgcaaaacgc gaaatcacaa acgcttttct tatcacgtcc gacgacatta tcatttgcga
    11341 ccccgaagcc gaatattacc cccttgtcgg acgtttgaaa ggacaggtta tcaaggtttc
    11401 gcagaacagc acgcagtaca ttaaccctat ggatattaac ctcaattaca gcgagggcga
    11461 cacgcccata gccttaaaga gcgattttat cctttccttt tgtgagttga ttatgggagg
    11521 taaaaacggg ctggaagctg tcgaaaagac cttgattgac cgcgctgtta tcagcgtgta
    11581 ccgcaactac cttgccgacc cgaagccgga gaatatgccg attttgggcg acctctacga
    11641 cgagatcaag aagcagccgg aaaaggaagc gcagcgtatc gcggcggcat tggaactcta
    11701 tgtaaacggc agcttgaaca tttttaacca caggacaaac gttgacattc ataatcgcct
    11761 tgtctgcttt gatattaagg aactcggtac gcagcttaaa aaagtcggta tgcttatcgt
    11821 ccaagaccaa gtatggaaca gagtaacggt aaaccgcagc gagggcaagg cgacgcggta
    11881 ttacatcgac gaatttcatt tactcttgaa agaggaacag accgcagcat acagcgtcga
    11941 gatttggaag cgttttagga agtggggcgg tattccgaca ggtatcacgc agaacgtcaa
    12001 agaccttttg acgagcagag aagtttcaaa tgtttttgaa aacagcgatt ttgtctatat
    12061 gctcaaccaa gcccagggcg accgggaaat ccttgccaag cagcttaaca tttcacaaca
    12121 gcaaatgacc tatgtaaccc attccgaagc gggcgaggga cttatcttct atggcaacgt
    12181 gattttgccc tttattgacc gttttccgaa agatacggag ctctaccgcg taatgacgac
    12241 caagcccggc gaggtgtcgg catgaggata gaaacggata aaatctattg cggcgacagc
    12301 ttgcaggtgc ttcaaacctt gccggataac tgtttggatt gttgcgtaac gtccccgccc
    12361 tactatgctt tgcgcgatta cggcacagac gggcagatcg ggcgtgaagc aacgccggag
    12421 gaatatgttt cccgcattac ggcggttttc catgaggtaa agcgggtgct tacgccggag
    12481 ggtacttgtt ggctgaatat cgcggacact tattgcggca caggcagcaa agccgaccac
    12541 caagacccga aataccccaa aggcaggaac gggcagcaag tggcggtaaa ccaccgcgcc
    12601 cccggctgca agccgaaaga cctaatcggt attccgtggc ttgtagccct tgccttgcgg
    12661 ggcgacggtt ggtatttgcg aagttctatc atttggcaca agggaaacgc catgccggaa
    12721 agcacgcggg acagaccgac ccgctgctat gaatatgtgt ttctgcttac caagtcaaag
    12781 aaatattact acgattggca ggcagtagcc gagccgatag cccccacaac ggcggtacgg
    12841 ctgaaaagcg gagttgggaa aggcaacaaa tatgccgcta ccgttccggg gcaaaaccag
    12901 ccgcagaaaa tcaaccgccc ccgcaggaaa ggcgcataca ccgacgaaat gatttccccc
    12961 gtccgcagcc gccgcaacgt ttggcagatc aacacaactt cctatcgcgg cgggcatttt
    13021 gcagcgttcc cgcccaaact tgcggaaacg tgcattttgg cgggctgtcc tgtcggcggg
    13081 attgtgcttg acccgttttt aggcagcggc acgaccgcag cggcggcaaa aagcctttcc
    13141 cgccgctata tcggtataga gatcaacccc gaatactgca cccttgcaaa acagcgtatt
    13201 ggaggtgatg aacattgagc aaggaattaa aagcccccga caaggtaacg cagaaaatga
    13261 cccgcgacgg ggcgattgcg gaaaacctta cgacgggcga aacgacgagt atcagcgaaa
    13321 gaccgccgga ggaaaactat tcagcccccg cagcgggagc agcggaaaaa gcgacccaac
    13381 gtatcagcac agaggttagc cgccatttag aaaagggcgc ggagaaaaag agccttgacg
    13441 cagcgcagct taaaacccat acttcccgct tgcagttttc cgcagaggaa cgggcaacgc
    13501 cggaacttca aaaggcaatt aggaagtcgg acaaggcggc agaccgttta gacgcggcgc
    13561 gggcggctat ccccaagcat accaaaatca agaaagagcg cgtttttaac gaagcgaaag
    13621 gcagggcaaa aacgcgcctt tcctttgaaa agacggataa gcccccaaac ggcaagttgc
    13681 ggcataaccc cttatcacgt ccggcgcagg agttaaacag caccgtacat ggaaaaatct
    13741 acgaggtaga gaaagaaaac gtcggtgtgg aaagcgggca ccgggtagaa cttgcgggcg
    13801 agaaagcggg cggcatggcg gcgcggacgg taaaacgcgg catacgcagc cacaagctga
    13861 aaccctaccg ggcagcggcg gcagcggaga gaaaggcggt aaaggcaaac gccgatttcc
    13921 tctatcaaaa ggcgctgcac gataaccccg cccttgcgca ctctaacccc atttcccgtt
    13981 tttggcagaa gcagcgcatt aaaaagcagt atgcaaagac gctgaaagcg ggcggcaagg
    14041 tgaaaaagac cgccgaagcc accgcaaagg cagcgaagaa aaccgcgcag gaaaccaaac
    14101 gggcgacgtt ctttgtcgtc cggcattgga aaggctgctt gcttgtgggc gggattgcct
    14161 ttatcgtgct gctgcttttc ggcggcttgt cctcttgctc cctctttggc ggcaacagcg
    14221 gtagcggctt gattgcgtcg tcctatcttt ccgaggacgc ggatattacg ggcgcggaag
    14281 cagcttacac cgatatggaa gcagagttac aggatatgct gaataatatc gagcgcgaat
    14341 accccggcta cgacgaatac cgggtgaacg cagacgagat cgagcatgac ccctatgtgc
    14401 taatctctat cctttccgcg ctgcatgagg gcgtgtttac ccttgatgaa gcgcaaagca
    14461 cccttgaaat gctctttgag aaacagtata tcttgaccgt tacggaggaa gtcgaggtac
    14521 gctaccgcac cgaaacgagg acggacagcg agggcaacga atatgaggtt gaagtcccat
    14581 ataactatta cattcttcat gtggatttgg aaaacttcaa cctttcccat gtccccgtct
    14641 acattatggg cgaggaacag ctatccatgt acgctatgta tatgtcgtcg ctggggaacc
    14701 gccccgattt gttcccgtcg tccggctatg tgtcaaaata ctatgaaaac ccgcccgccg
    14761 attacgaagt acccgccgcg ctgcttaatt ccgatgaaaa gtttgcgcgg ctcattgagg
    14821 aagcggataa atacgtcggc tttccctatg tgtggggcgg cagcaccccg gaaacctctt
    14881 ttgattgcag cggttttgtt agctatgtgc tgacgaacag cggcttatac aatacgggca
    14941 gacttggggc gcaggggctt tacaacattt caacccgcgt ttccaacccg cagccggggg
    15001 atttggtttt ctttacgggt acatacgaca cgccgggggt atctcatgtg ggtatctacg
    15061 ttggcgagga cggggacgga agccccgtca tgctgcattg tggcgacccg atacaataca
    15121 caaaacttga tacaagctat tggcaatccc atttttacgc ctatgggcgg ctacattaca
    15181 actgactttg aaaggagtta atttctatgg caaacacgaa acttgaccgt atcgaacggg
    15241 atattgagaa aacgagggcg aaaatccttg aataccaaaa aaggctgaaa gaccttgaag
    15301 cgcagaaaat cgaggaagaa aacgcgcaga tcgttcaaat ggtgaaagcg gtacaccttg
    15361 acggggcgca gcttgccgcg ttcctctccg cttacgccag cggcgaaatt gccttgccgc
    15421 agccggacgc agactatacc gacgaacagg aggaaaccga agatgaagca taaaaaactg
    15481 ttccgcagcg ttaccgcttt gcttgccgcc cttgtactca tgggcggctt ttctgttacc
    15541 gcttttgcgg gcggcggtga tgtgagcgac gagccgccta cccccgtaac cgaaaccgag
    15601 ccggaagaaa cgacgggcgg ctatgagccg cagcccttaa cgccggaggg caacatgagc
    15661 cttgtggacg acataacggg cgaagcgtcc ggcgataaac agtttattac cgtcgttacg
    15721 aaaaacggaa actattttta catcatcatt gaccgagccg aggacggaga aaacaccgtc
    15781 catttcctca atcaagttga tgaaaaagac cttttgcagc ttatggagga cgaacagacc
    15841 gaaacccccg cttgtagctg tactgaaaaa tgcgttgccg gaagcatcaa caccgattgc
    15901 cccgtttgca gcgtcaacat gagcgagtgc gcgggcaagg aagccgagcc ggagctggaa
    15961 gaaaccgagc agccggagcc ggaggaagat aaaggcggcg gcatgggcgg tgctattctg
    16021 cttgtcgtgc tgcttcttgc cgcagcgggc ggcggcgcgt tctattactt taagattatg
    16081 aagcccaaaa aggacgcggc aaaaggcagc agcaaccttg acgatctcga ctttgacgag
    16141 gacgacgagg acgcggaaga attagaaacc gagcaggagg acgaggaagt atgaaacttg
    16201 taattgcaga gaaaccgagc gtcggggcgg cgattgccgc cgtactttgc gcgaatgaaa
    16261 agcgcagcgg atattttgag ggcagcggct accttgtgtc ctggtgtatc gggcatttga
    16321 ttagccttgc ggacgcggcg acctacaacg agcagtaccg aaaatggaag tatgacgatc
    16381 tccccattgt cccgcaggat tggcagttta tcgttgccag cggcaaggag cagcagtttt
    16441 ccgtactcaa agaccttatg caccgcagcg acgtttccga gattgtcaat gcgtgcgaca
    16501 gcgggcgcga gggagaactc atttttcgat ttgtctacga acaggcaaat tgcaaaaagc
    16561 ccttttcccg cctttggatt agcagcatgg aagaaagcgc aatccgcgag ggcttttcaa
    16621 acctcaaaga cgggcgcagc tatgacaacc tctatcaatc cgcgctttgc agagcaaagg
    16681 cagattggct tgtcgggatt aacgcgaccc gccttttctc tatcctctat cacaaaacgc
    16741 tgaatgtcgg cagagtgcaa acgcccaccc ttaccatgct ggtaaaccgc gactatgcga
    16801 tcagcagctt taagaaagaa aaatatcatg ttgtccgtct ggacgcgggc ggtgtttcgg
    16861 ctttgtccga aagactgaac gacgaagcgg cagcgcagca gatgaaagcg gcttgcgaga
    16921 aatcgcaagc cgtttgtact tccctcaaaa aagagaaaaa gaccgttgcc ccgccgaagc
    16981 tgtttgacct taccgcgtta cagcgcgaag ctaaccgcct gtatggattt acggcgaaac
    17041 agacccttga ttatgcgcaa gccctctatg aaaagcggct tttgacctat ccccgcaccg
    17101 acagcaaata tatcacttcc gatatgcagg acagcacaaa ggaactcatt accgggcttt
    17161 gttcgctgct tccctttatg cgggacgtga agctgcaagc agaccttata agggtttgcg
    17221 acaacagcaa agtaaccgac caccacgcta ttttgccgac agccgagttt ttgaaagcgg
    17281 gctttgcttc ccttgccgac agcgaaacaa aacttatgac ccttgtgtgt gcaaagctgc
    17341 tttgcgccgc agccgcgccc tatgcgtatg aagcggttac ggcggttttc acttgcggcg
    17401 gctatacctt taccgcaaag ggcaggacaa cgctttgcga gggttggcgt gagattgaaa
    17461 gattatcccg cgccgcgtcc gaggaacagg acgaggacgc agagccggaa gcggttttgc
    17521 ccccgcttac cgagggacag acctttgaaa ataccgcagc ggagatctcc gagcgttaca
    17581 cgcagccccc aaaagcatat accgaagata cgcttctgtc cgctatggag aacgcgggca
    17641 aggaggaaac gccggaggac gcggaacgca agggattagg caccaccgcg acgcgggcgg
    17701 gtatcattga aaagctcatt tcagcgggct ttgccgagcg caagggcaag aagctaatcc
    17761 ccacaaagga cgggtataac cttgtcgcca ttctgcccga aagccttacg tccccgcagc
    17821 ttacggcaga atgggaaacg cgccttaccg ggattgcaaa gggcagcgac agccccgccg
    17881 actttatgcg agggattgag gaaatgacag cggggcttgt caagacctat tccgcgattt
    17941 ccgaggataa agcaaaactg tttaccccgc agcgggaagc aatcggcact tgcccccgtt
    18001 gcggcgcggc ggtttacgag ggcaagaaaa actactactg ttccgacagg gcgtgcagct
    18061 ttgtcatgtg gaaaaatgac ttgttctttc agcagcgcaa aaagactttc acaaaggcga
    18121 ttgccgccgc ccttttgaaa gacggaaagg taaaaatcaa gggtatgtac tccaccaaaa
    18181 cgggaaagac ctttgacggg gttgtgctgc ttgccgacac aggcggcaag tatgtaaact
    18241 tccgcgtcga gcagaaccga aaatgattag gcttgatgaa aagcagtatc gagccgttaa
    18301 gaaaatggcc cgagccgcgt gcgccaacaa tgattgcggg aactgtctgc tattggacga
    18361 cggggaaact tgcgtttgtg tgcagagtat cagctattcg ctgttatgcc gttatttccg
    18421 cgaagcggtt ttacccgcag acaggcagct atgcgaacag atcacccgca gcggggaaac
    18481 cgatttgaag cgttgcgcgg tatgcggcag cacgtttgcg gcgggcagca atcgggcgaa
    18541 atactgtccc gattgtgcgg caaagatacg ccgcaggcag aaagcggaaa gcgagagaaa
    18601 caggcgttta cggataaaaa taactacata gcaagcacag caaacgagta cccgtttgcg
    18661 agaaataccc gcgctgctct atgggaagcg cgggcatttt gcttgttggg atagtcccaa
    18721 acccttaaaa atcgagaaag gacgtgatga aacaatatgc cgtattcttc atacgaccat
    18781 gacaaattgg aaaccgccga aaccatgcgg atagaacgcc ggatatattt tgaagcaaag
    18841 gacggggata ttgcccccta tgtttctttg ccgctggaac agctacacgc catgcgtgag
    18901 gaaagcgcgg cagcggaaca ggcgattttt aacgatctct caacccgcgc cgccgcttgg
    18961 gaacagcagg cgggaaaaac gcttttgctt gacaaagcga ttgaatacac aaggacaacc
    19021 gttgtgcagc acacttctaa cgagtggcag aaaggcgaat atgaccgata tacccgcagc
    19081 aaccgggtat atcagatgaa ctaccacatt tacgagaaca cccgttacga cagggagaag
    19141 cagcaaagcg tcccttactc ttattctctc acatggggcg tttataccaa cagcccgaac
    19201 aggaacgggc aagcgaagat tgccggacag gacagaaagg ttttttccga taaggcggct
    19261 atggaaaagt atctgaatgg acgtatcaaa gcatacgacc gtctgtttac ggaaatctcc
    19321 ccgcctatcc cgcaggagta cgccgagcat ttcaaagtaa acggtatgct tttaccgggc
    19381 tataccattg agggtgagga acccccgcag cagcagccac aggcggcagc tatccccgaa
    19441 actaccgggc agcaaaagga gcgtgaacac atgagcgaac agttttctat tatgattggc
    19501 aaccgcagcc gttttgaagc gggcgacccc ggcggttatt ggctggatat gcccgccaca
    19561 aaggagcagt tacagaccgc tatgcagagc gtcggcatta ccgccgataa cccgcaggat
    19621 ttttccattc gcggctattc cgacgaccca gagaaacata ttgctttgcc ttatgaaatg
    19681 gtatgcgccg ccgacgtgga cgaactcaat tttcttgcgg cgcggctcga acagcttgac
    19741 cccgccgagg ttggcaagct gaacgcagct ttgcagcaga aaaacgggct tgcaaatatc
    19801 ggacaggtaa ttgacttcac ctacaacgtg gatttttacg tccatattcc cgaagtacat
    19861 acctaccgcg atttgggcga ctattactta aatcaatccg gcatggtaca aatgcccgaa
    19921 gaatggaaag gcggcattga ccttactact tttggcagga acgccgccgc gcaggaaaaa
    19981 ggcgcgttta ccgaatacgg ctatatcgtg gaaagcggcg acgagtggga acggcagttt
    20041 gaggggcgcg aagtgccgga ggaataccgc attatgagtt acccgcagcc ggagcgcggc
    20101 gaacaggaca aagcctatat ggacgcagcc gaaacgcagc aagcagccgt acagaccgca
    20161 gagccgcagc agccccgccc cgtcgtgccg attatccttt cttctgaaaa acccggcgac
    20221 aaggtaaagg agatcaccgc ccgtctggaa cagggcatac aagcgatttt tgacagcgac
    20281 cgctacaaag agtttttaac ggctatgtca aagtttcacg attacagctt gaataacact
    20341 atcctaatcg ccatgcaggg cggcaatttg gtaaagggct attcacaatg gcagaaagag
    20401 tttgaccgcc atgtgaaaag cggcgagaaa ggtattaaaa tctttgcccc cgcaccttac
    20461 aaggtaaaaa agctggtgga taaaatcgac cccgacacaa gaaagcccat acttgacagc
    20521 gagggaaaga cggttaagga agaaaaggaa gttaccgttc ccgcctttaa ggttgtaacc
    20581 gtctttgaca tttcgcagac cgagggcaag gagtttcccg acctctccgt caaaccgctg
    20641 cttgccgatg tggaacagta cgaggacttt ttcgctgccc ttgaaaaagc gtcccccgtt
    20701 ccgattgctt ttgagcagat cacaaacggc gcaaacggat atttcagttt gaccgataag
    20761 cgaattgcta tcaaagaggg tgtgagcgag ctgcaagcgg ttaagaccgc cattcacgaa
    20821 atcgcccatg cgaagctgca tgacgtggac ttaaacgccc cgctggagga acaaaaccgc
    20881 gttgaccgcc gcagccgcga ggtacaggct gaaagcgttg cctatacggt gtgtcagcat
    20941 ttcgggcttg atacgtcgga ttactctttc gggtatgttg ccgggtggag cagcggcaag
    21001 gaaatgaccg aattaaaagc gtcccttgaa acgatacagg caaccgcaaa ggaacgcatt
    21061 accgagatcg aggggcattt taccgaattg cagcagcagc ggcaagccga gcaggagcag
    21121 gaagcgaaaa caaaagaaac cttgcttctc aacggcaagg aggacagcta cgggatttat
    21181 cagttagccc gcagagagga aactatggat ttgcgctttg agcctttcga ccgtctgcaa
    21241 gcaacggggc atttcgttga ccgggcaaat tatgaactcg tctataccgc accgcttaca
    21301 aaggatatga cgcttggcgg catttgggaa aagtttaata tcgaccgccc cgccgacttc
    21361 aaggggcata gcctttccgt ttccgacatt gttgtgcttc atcagaacgg cgagaacacc
    21421 gcccattatg tagacagcat aggttttcag caggtgccgg agtttttaca ggagcaagcg
    21481 cagcagaccc ccgtatttga taagctatct cccgaacagc agcaagccct atccgatacc
    21541 gtaaaagata cgctgcaact gcttgtagac gcagacaagc ggatttatgg cgacgttacg
    21601 ggcaagaccc ttgaagcaat cgcggcgcaa gggtattcct acaagggcgg gcagcttgaa
    21661 aagcagcagc cggaagcgac ccccgaaagc cttatgacgg gcgaaaccgt cagaacgccg
    21721 cgagggaact tttacattac cgacatgagc cgcgaacaga tcgaagcggc gggctttggt
    21781 tttcatcatg cgtcggagga cgggaagtat ctcattatgg gaaatggcac acaggcttat
    21841 gccatagccg ccgagcagcc gcagcgggat aatcccttaa agcacgttga ggacattgta
    21901 gagcagaacg acaacaactt tgacgggatt atcaataata cgccgcaaac gcccaccgtc
    21961 gcggatttgg agcagcgggc aaaggcgggc gaagcgattt ccgttaccga ccttgcgaaa
    22021 gcggtaaaag ccgagaaacg ggagcagccg caaaagaaac cgtccatttt gaagaagctg
    22081 gacgagtaca agaaacaggc ggcgcagcag ccgaaagaca agcagaaaga gcagaaaaag
    22141 gatttggagg tttgatagta tgaatatccg ctttaccatt gaggaagaaa acataattgc
    22201 gatttacgcc gaggacagcc gggaaacaac gcttgaaaat atccttgccg ccatgcccta
    22261 tatggacgag gatatgcgcc agcttgccgc cgcagcggtg aaaaagctgc aagccatgac
    22321 ggacagcgag ttttccgaac gggagtttat cttgaccgac gaggaatagc ccatagaaaa
    22381 caggggcgcg gttgccgatt tccggcaccg cgcccctgtt ctttttttgt ccttgcgtgg
    22441 acaattagaa agctgttttt gcgggtttga atataaagta tcgtctatgc cgtttccccg
    22501 cgctggaagc cgcagaaaca caggcgtttt cagcccttaa tgttccactt gctacccctt
    22561 gttttgcgcc ttatcgctcc tgctcccgcg ccttgctttg ggacgggtaa agaatactat
    22621 cgacgttctt tttgacaacg ccgtactcct gcatttgctt tttcgcttgg cggtaatcct
    22681 catacaattt gcttttcctg tcgtttaagc cgctatactc cttgcgcagc gcggcgagat
    22741 cgggcagctt ctttatctgc atttcccgga gtgcccttgc cgcagcttca aacaggataa
    22801 tttcgctttc atgcccccgc agatactttt ccttgtcccg cgatttcttg tattcctcgt
    22861 aaatcggttt taattgtcgg tatgtggtgg tgtgcttgat aagcagcgat aaatcggaca
    22921 tacgctgttc cacttcctta accgcccttg ccgccgcgtc atgggcggtc ataatatccg
    22981 cgattttgct ttcgaggtcg ctgtaatatt caatcttgtt ttcggtcaag aagttcatgc
    23041 tttttgcagc ctgtttcaga ttatggattt tcgcccaccg ttcatagccc gccgattgtt
    23101 gggctttaat gctgttttca atatcgacga caagccggat tttcttatct tcccgcagcg
    23161 gctttgactt ggcaatatat acgcccttga tacgctcctt tatggcttct tccgtatagg
    23221 ccgcgccgag cgtctttgcg cgggtaaacc gttcctgtcc ggcagcgcgg aaagaaatat
    23281 atttgccctg tttgatttca taccccattt cctgcaaacg gcgcagcagt tcgtcaaaat
    23341 ctttgacttg gggaatgagg gtatctatcg ccgttttcag cttggctttg tagcttgttc
    23401 cttgcttttc ggcggtgtat tccgcatagc ttttcccttt gtcctgtccc ggcacgacga
    23461 cggatagccc atgctcttta catatcctgt cgctggtgcg tcggatagcg tgatagcttt
    23521 gcttgttgga atgatacttt ttgtagtcaa cgaagctaac cgcgttgaaa atcaagtgat
    23581 tatgcagatg gtctttatcg acgtgggttg tcaaaacaaa ctcatacttg ccgcctaaca
    23641 ccgcttgcgc aagttccatg ccgattttgt gcgcttcttc cggcgtggtt tcgcctaccg
    23701 caaaggattg tatcaagtgc cgccctaaat gtgtgccgcg atctccggca gcttcccgcg
    23761 tccatgcaaa ctctatatcg gcggtttcca gcccgcagcc gaaagaggac gcaagcagct
    23821 ttccgtctgt cttttcggga tttaagatat agtctattgc ggcctttaag gttgatttaa
    23881 tagggtgcgt ctttgtaacc gccatatctc gtccaccgcc ttttttatat cctcaatgtc
    23941 ctgctggtaa accgcccccg tcgcgttggc gcgtttggca atttgattga tgttccggct
    24001 caccgcttgc agttcccgga tatatgcttt cgtgtcgctt cggtcaatga caaggatata
    24061 cccgtcaatc gccattttgc ggaagtatgc cccatactgc tttatgggaa gctgcttcat
    24121 cttctcgtcg atcagccgct tttcttcttc cgtaacgtaa aacttcattt ggatatttct
    24181 ctttcggttt tccatttccg cgccgccttt cggtataagg gtttgggatt atcccaacaa
    24241 gcatttttcg caaacgggta ctcgtttgct gtgcttgcta tctactccat acccctaccg
    24301 aaaggcggta tttgttgcgc attcccttga ttttggacgc aaaaaaagat tgcagccgcc
    24361 atgcgctgca atctcccgat ttctcatatt gtgaggttaa tcctctgctt tttcgacttc
    24421 cgttatctcc cttgctgttg cggttacaat ccgtatgcct ttatcgctca taccgtccag
    24481 cagcgcgtca agctgccgcc gcccggtgga tttctccgca ttgctgttcg ggaagaaaaa
    24541 ttgatctacc gaaatatgat accgggttac aaggtcatag aatacctgta aactcggctg
    24601 ttgtcccttg ttctcgatat tcgcaaggta gcgcggggaa atatataact cgtcgcttac
    24661 ctttttgcgg ctctcgccgt attcatttct cgcggctttt atagctgccc cgaaagcctt
    24721 aaagtcgtac aatggtacgg gtctttttgc cattttcatt caccctgtta cattttacag
    24781 ttcacctttc tttttggata tgtccacaca attcatatca ttaggttaaa tacttcctgt
    24841 tgtgtattga cagtagactg attttctgat aaaataaaat ttatgtaaaa ggaggtggcg
    24901 tttatgttgc atagcatgcg tattagataa gcattgaaaa aaaggatttt cccattttca
    24961 acatgggctt ttttgtcatg cttattttgc ggatgctata caaaaaacta acgacgtata
    25021 gatatagtgc aagctatgcc ttagttttgt tgtactgctc tgtgcattat aaatgcaggt
    25081 caatgctcat gttgaggatt gacctgcatt ttttgtgtaa aaaggacgag tgaaatataa
    25141 tgcttaaaaa atattggata aaatgtccga tttgtaacgg aaagacgaga gttcaagtat
    25201 ttcataatac tgtattaaaa gattttcctc ttttctgccc taaatgcaaa ttgacgcata
    25261 tcattgatgt agaaaaatta gagattgtaa tcaaaaatac agaaaaacaa acttttatta
    25321 tttagaagaa aggatataaa aatgaaacgt ttacctaaat atacgcctgc ggaagtacgg
    25381 aatgatccat acggatttac ttacaaagaa atgtcggaag ttattggcga gaatgaagca
    25441 aaagccttat atgaagaatt atataagcaa ttaccacgca aaaaaaatct atcaatgttg
    25501 gtaaaaaata tttgcaaaag cagtgatact gaaaagtatg tttacgaact gaaagacaac
    25561 aaatacattg aaacggtttt tatcaagcgg cgagatggtg gaactgtttg cgtgagcaca
    25621 caagtcggtt gtcctgttgg ttgtattttt tgtgagtccg ggcgaaatgg ctttgttcgt
    25681 aatctaacat cgtcagaaat cgtacaacag attatattgt tgcgtcgaaa agtaaaccgt
    25741 atcgttttta tgggtatggg agagccttta ttcaattatg acaacttgat aaaagcaatc
    25801 catattctcc gagatagata tgggctcaac tttccaaccg acggcattac catatcaaca
    25861 gttggtccgg tcgatcaatt aaaaaaattg cgcgaggaac atcttaaaat tcagttgaca
    25921 atatctttac acgcagcaac acaatctgca agaaatcgta ttattcctca catgcgcata
    25981 tatgctattg aagatgttgt taagcaagcc ttatcctatt ctgaaaggca taatcgcaaa
    26041 attgtctttg cgtatttgct tttaccgggt ataaatgacc ggccctcaga tgtaagacaa
    26101 cttgcaaaat ggtttcgggg caaaaaagtt atgattaacg tgttacaata caacccaaca
    26161 agcaattcaa gaattaaagc accacagaaa cgggaaatag ttgcattcaa acatcaatta
    26221 gagcaagcag gacttgaagt tactatgaga gtttctcatg gcagagagat taacgcggct
    26281 tgtggacagt tagctaacac atataataaa ttcaaaaaaa atgattaaaa gtgccagacg
    26341 catagacgca gagccgataa cgcattaggt caaaaaacct atgtgttatc ggctttttta
    26401 tttaatattg cgcaaaagcc gctgttccct attatagaat ggattgcggg tagtgtatta
    26461 aagtcgccct tttttaagga tacggcggta tcggggaggt atcgccgtgt ccatttttat
    26521 ttactgtaac ccgcctaatg ctttgcggtc aaaaaaagct atgctccgca agcgggatat
    26581 tccggctatg aatgtgaact gtcaatacat ttagctactg cttacatttc ctttgcgccc
    26641 gtccgcgtct tgcggacggg cgcattttgc ttttttcaac ttttttctga aaagcgcact
    26701 caagagccat ctcccgaacg ggtacggagc gaaaggggca gagaggacaa gcccttaact
    26761 cccgtcctct actccacgcg ggaaggaggt gaactctatg gagctatctt cttccgacaa
    26821 ggaaagaata caacatcagt acgacgcatt agcaaagaaa actttggtcg gcgaagcgaa
    26881 aagccaccgc cgcactcttg cgaaacgcgc agcacgcgaa gttacttttt cggatttgag
    26941 cgaaagcgaa ctcgcgcagc ttttcacaac ggacgaatac gaaagcgatt atttccgttt
    27001 tcaagtgtcc ggctttgatg tactcgtcaa aaatgaactg cttgccgaag cccttaacgc
    27061 tttgcccgaa aggaaacgcg acattatcct tttgtcctac ttcttggata tgagcgacgc
    27121 ggaaattggc gaactgctga atgttgtacg cacgacggtt ttccggcaca ggaaatccgc
    27181 gcttgcgaaa atcaaacagt atttggaggg aaaagcagat gatgaatacc gttaggaagt
    27241 ctgaaaatct gttgccgttc cctgtcattt ccgcagcggc aaacggcgac acaaccgcca
    27301 tgtgcgcgat cttgaagcac tacgagggtt acatagcgaa actttgtacc cgcacgctga
    27361 aagacgacgc gggcaatacc tattcctatg tggacgagga aatgcgtaac aggctgcaag
    27421 tgcgccttat tacccgcacc cttgcttttc atgtaggata atcttttagc ccatgcgggg
    27481 agcgtgtccc ctttccacgc ttcccgttat gggctatttg tcgttccgca aaagcatatc
    27541 cgctgttgat gtgcttttgc aggccgacaa agcctattgt tctttgacaa agaaagcggc
    27601 ctttggtgcg cagcataaca ggcaaacggg tacattccgt ctgtaccgag ccagtcggcg
    27661 ggtacgccat gacagctttt cccctacggg gaaaagtcga gcgacaacta ccgcgccgta
    27721 aaacaggttt agcagcttgt gggcgacgac atacaaggcg gcacaatgat actcccgcgt
    27781 tgaacaccct caaagtattg cgcggcgcac ccataagcat gggccggggt gaaactcccg
    27841 tgaggttgca gctaacaacc gcccgtttca gccttatttc tcatatcgta caagccggag
    27901 cgcatattct gtactatgcc gctaaccggg agggaaagaa gatgattacc aagttacaac
    27961 ttcaacaaat gagaaatgtt gatattacac aggttgaccg cagcactttg gttgatattc
    28021 gtaatatcca tattgacagt tctttgccag cagcaaaaaa aatgcaaagc tacttggagc
    28081 agatcgtcaa tccgtattgt tttctttgcg gcgatacgcc cgtaagaata cggtttgtcg
    28141 cagaggatag aacgctaaag caatcattgt gcgattactt tttaagtctg aaataaccgc
    28201 cgactttccc taactctatg ggtaagacct tgtgattacg gggcgaaaat ggtataatta
    28261 actcgtaatt atagggggaa aggaggtata atgccccgca tacgcaaaga caaaatggca
    28321 cgcagttatg cagagccgtt ttggaaaatt gggctataca ttcgactatc cagagaggac
    28381 gacaacgagg acgaaagcga gagtgttatc aaccaagaaa agatactccg cgactttgta
    28441 gacagctatt ttgagccggg aacctatgtg attgtagacg tgtttgccga cgacggattg
    28501 acaggcaccg acacagcgcg accaaacttc aaacggcttg agggctgcat tgtccgaaaa
    28561 gaagtaaact gcatgattat caaatccctt gcacgcggtt tccgcaacct tgccgaccag
    28621 caaaagtttt tagaggagtt catacccatt aacggtgcaa ggtttatttg cacaggcaca
    28681 ccatttattg acacttacgc aaatccccat tctgcaagcg gccttgaagt acctattagg
    28741 ggaatgttca acgagcagtt tgccgcgacc acttccgaag aaatccgcaa aacattcaag
    28801 atgaaacggg agcgcggcga gttcataggc gcgtttgccc cttacggcta caagaaagac
    28861 ccaaacgaca aaaacagctt gattgtagat gaagaagcag cggaggttgt caaaagcatt
    28921 taccattggt ttgtcaatga ggggtacagt aaaatgggga ttgcaaagcg gcttaaccaa
    28981 atgggagagc cgaaccccga agcctataag aagaaaaaag gctttaagta taacaaccct
    29041 aattccgaca aaaacgatgg tttgtggtct gccagcacga ttgtaaggat attgcaaaat
    29101 gaagtttata cgggcgtaat ggtgcaaggc cgtaatcgcg taataagcta caaagtacac
    29161 aagcagatca acgtccccga agaagaatgg tttgtcgtcc ccaacacaca cgaagcgatt
    29221 attgacaggg aaacattcga gaaagcgcaa gccctacata agcgcgacac aaggacagcc
    29281 cccggcaaac aggaagtcta tctcatgtcc ggctttgtcc gttgcgcgga ttgcaaaaag
    29341 gccatgcggc gcaaaaccgc ccgcgatatt gcctactact cttgccgcag ctacaccgat
    29401 aagaagattt gcagcaaaca ttctatccgg caagacaagt tggaaaacgc ggtattggca
    29461 gcgttgcaaa tgcaaattgc ccttgtagat cggcttgcag aagaaattga acggattaac
    29521 aacgcgcctg ttatcaaccg ggaaagcaag agattatccc attccttgaa acaggcagag
    29581 aagcagctaa aacagtacaa tgacgcttcc gatagcttgt accttgattg gaaaagcggt
    29641 gagattacca aagaggaata ccgccgcttg aaaggcaaga ttgcagaaca gatacaacag
    29701 cttgaagcga acatctccta tctgaaagag gaaatgcagg taatggctga tggtatcgga
    29761 accgacgacc cgtatctaac cgcctttctg aaatacaaaa acattcagag ccttaaccgc
    29821 gggattatgg ttgaactcgt aaaagcggtt tgggtgcatg agaacgggga aataacggtt
    29881 gacttcaatt ttgccgacga gtaccagcga attattgatt acatcgagaa taaccacaac
    29941 gttatccgag tgattgagaa caaggcaatc taacggcttg cctattcaac acaaaggaaa
    30001 atctaatgtt gtgcataaat aaacggctct actgctcctg ttggacctat tgccccagct
    30061 actcctgttg ctcctgtcgc tcctgttgga cctaccaaac catttgctcc ggttgctcct
    30121 actgctcctg ttggacctac caaaccattt gctccggttg ctccagttgg gcctgttggg
    30181 cctgttggac ctaccaaacc atctggacct gttgctccta ctgctcctgt tggacctacc
    30241 tctccatctg ctccggttgg acctgttgct cctattgggc ctgttgcccc agttgttccg
    30301 gttgctccag tcgctcctat acttccggtt ggacctgttg ctcctgtatt tcctgttggt
    30361 cctgtcgctc ctgttactcc atttgctccg gttgctcctg tatttcctgt tggacctgtt
    30421 attccatttg ctccggttgc tccggttgct ccagttagtc cggttggacc tgttgctcca
    30481 gttgctcctg tattccctgt tggtcctgtt attccatttg ctccagtcgc tcctgtattt
    30541 cctgttggtc ctgtagagcc tgttattcca tttgctccag ttgctccttt gtttcctgtt
    30601 ggtcctgtta ttccatttgc tccggttgct cctgtattcc ctgtcggacc tgttattcca
    30661 ttcgctccag tcgctcctgt atttcctgtt ggtcctgtag aacctgttgt tccatttggt
    30721 ccagtcgctc ccatatttcc tgttggaccc gttattccat tcgctccggt tgctcccgta
    30781 tttcctgttg gacctgttat tccatttgct ccggtcgctc cagttgcacc tctaggcccc
    30841 gtcggtcctg ttggaccagt agcacctgtt ggccctgttg gacctacatc ttgatataaa
    30901 cttggacctg aaatatcact catataatta atcctccttt tttaaagtta gagtattact
    30961 ctataaatat tgagtattac tctatataat tatatgaatc gacaatttct tatgtgataa
    31021 tgtatctatt tgcttatatc aacatatatg ctttgattct tgaatttcat tacttttttg
    31081 taaaatcttt tacaattatt agtactcttt tacaattatt aatacaatta aaaaactcat
    31141 ttcccatcaa ttatttaaaa tttttttatt atgttataaa aaatagataa ttaaattgca
    31201 taaaattaat ttaatactac tttaattact ttttaaataa aaaaacctca acacgattaa
    31261 taaaaatcat attaaggcat ccacaaaatt atataattgc ttatctatct tcatcagtaa
    31321 cattgaatat ataaggaaca tttctataat aatcaccaaa atcaaaccca tatccaacca
    31381 caaacttatc ttctatttca aatccacaat aatctggcac taaatcgact tttcttctgc
    31441 ttggtttatc caaaagaaca cagcatttta aactagcagg atttttagct tttagatgtt
    31501 ttaacacaaa atccatagta agtgcagaat cagtaatatc gtctacaacc aaaacatcat
    31561 atcctgctat atcagtagtc acatctgaaa ctactttaac ccttccagta ctttcttcat
    31621 tgtttccata actagatgta gtcataaaat taactctaag tggtaaattt atatttctaa
    31681 ctaagtctgc acaaaatata aagctacctt ttaatagaga aactaccata agatttttat
    31741 ctttataatc tttttctata attgctccta attcccttaa tttatcttta attttttctt
    31801 ctgaatacaa tacttcccat ttttttgttt ctatatccat aaaaagacct ccaattgtaa
    31861 taatccttct taattttata agagattgct cactttttca atactcttat tcaatattag
    31921 tcaatattat tgagttttta tgttcattca acaattatta acaatgtatc ttatatttta
    31981 agtagttcat atatccacac taaattacat actatatatt ttagacaaaa aaagaaaaaa
    32041 cttgcactaa caagtttttt ctattataca taaatttata cataatcata ttttattaga
    32101 gggtactaat taaatttatt tatgctattt ttgttatacc tcataataca aaacatatta
    32161 taaagtacta gtcaacttta ttcatatcat tcattacatt taagattatc tcatctgtta
    32221 tagacatacc tttgtcactt actctaccta agttttgtat actttgctca acacttgaac
    32281 caactatacc atttagtggt ggtacaaaac aatcttgctt agctattata gcactttgta
    32341 tagctgcaga tgctgcagaa gataatttta atgcacaacc agctttcgca ccgtcacata
    32401 tcattccact taagtcagct atcatatttt ctatagctcc ttctatttgc ttttcagtac
    32461 cattcataag ccaagagata ccagcagttg ctccagtaga agcagcaaca ccacatccac
    32521 atacagcaga taatcttcct gtatagttct taatataacc agttactaga tgagatatag
    32581 ctaaagcttt tgctagtctt tcatcagatt gtgggaattt tttattataa gcaacgattg
    32641 gaagaattgc tgttaatcca tgatttccac ttccatttga actcataaca ggcattttta
    32701 cccctgccat tcttgcatct gaagctccag cagttaacat cattgcataa tttataacat
    32761 cattacctaa aagaccttct tctatagact ttttaattcc atatcctaca ccaataccag
    32821 ttttttgttt taatccatat tcagccatct cttcattcat ttttactcca tctagtaaaa
    32881 attctatatc tttaaaatca attccttcta cattttttat taattcttgt atagttactg
    32941 tatccattaa actttcagct ttttctgccg catcatttga agctgatact tttggttcat
    33001 tgtctaataa tacttctcca tctttttcta agaacgtaaa attatcatgt ttagttctta
    33061 ttctcacttt agcaatatga ttttttcctt ttaacacaac ttctataaat actttctctc
    33121 ttgtatcagc aggtgttatt gatagtggag tattatccat ataatcctca gctattttaa
    33181 cttcctcttt tgttagagtt tctaacacac ttaatccatt ttcagaatgt ccacctacta
    33241 tacctagagc tgctgcaatt tttaatccta atctttcagt acctggtata cctacgcaca
    33301 taccattttt ataaatacta ggactaacta acatacgatt ttcaactatt tcttctccta
    33361 ataattcctt tgcctttgca catgctagag ctaaggctac tggttcagta caccctactg
    33421 ctggtttaac ttctgccttt aacatttcca ctaattcttt tcttatatct ctcattatat
    33481 ataaacctcc caattaagtt tagatttagt actcaaataa ttaattaaat aatttcttat
    33541 ctatatttat agcaaaatac atgccatatt ttttatatct ttttatattt ttagtcttat
    33601 attctataac cattggtatg actgcttcta tttatgtatt ttttttattt acatagagta
    33661 tttatattct ttaaaatgga gaaacatatg acaatttcgc aaaaagacaa aaaaaagtct
    33721 caaaatgaga ttctatatca ttttgagacg gctcaaatgt attatttttt aacttataaa
    33781 aaccattttt taattcataa aaaaagattc tgcaaaacac ttatagtata agtttaaagc
    33841 tttctttaaa gttatgaatt tagaaaaatt taaattttat atagcttttt attatattac
    33901 tatgaaatta tattgtattt ttcaattttt ctatataatg tagctcttga tattccaagt
    33961 gctttagctg ttaattcttt atctttttta taatctttgt acttatttaa agcttttatt
    34021 atctcaatct tctctagttc atcaagtgga attatcctat cgcttttaac ttctaactct
    34081 tctacagagg tattcttaat tttttgagga attacattca ttgtgattat tgaagaagaa
    34141 gacatattca cactatattc aataatattc tgcaactctc ttatattccc tggccactta
    34201 tattccatca tagcatcaac aaccctgcta tctatgtcaa taacattttt atctaatttt
    34261 tttgaatact ctttaaccat atgcttgatt ataagtggga tatcttcttt tctctctctt
    34321 aaacttggta attttatagg tataacattt aatctatagt ataaatcttc tctaaaagtg
    34381 ccattttgaa ccattttttc taaatcttta tttgtagcag caataattct tacatctatt
    34441 gaaacatttg atttacttcc aattttatta agttcctttt cttgtaatac tctcaataac
    34501 tttgactgta aatgcaaact catatctcct atttcatcta aaaatatact tcccttatga
    34561 gctacttcaa acattccaag ctttccacct tttttagctc cagtaaaagc tccctcttca
    34621 tatccaaata attcactctc taataaattg tctggtatag cagcacagtt tacagctata
    34681 aaaggattat ctactctatt gctatggttg tgtatggctc ttgcaaatag ctccttaccc
    34741 gttccacttt ccccagtaat aagtactgtt gatgttgatt tagaagctat tagggcttcc
    34801 tttttagaat ttcttatagc tacactttct ccaattatgt tttccaattt tattctataa
    34861 tctttattca tcttattgta gttttttata gcatctctct tatctataaa atcaataaca
    34921 tatcccttta actcattttt taatactatt ttattgatgt tgtatattcc cttcgaatca
    34981 tatccgtatc ttttataaaa aaaactacag gatttgtcct tcttaaagtt atttatagaa
    35041 ggtttcttta taaaatctaa ttcgacaaat atgtttttgc cagttatact atcttttata
    35101 ttgaaaagct ctttaaattt tgaattatat ttatcaattt ttccgtccac atcaatagac
    35161 acaacagctt tgtccatatt gtttaatagt gtctctaatt ttttcttttc taattcaagc
    35221 tcataagttt taatttgtgc ttttaattta ttagatatta ggtctgccat tttgcctaaa
    35281 aagtttataa gaccactttt attattttct ataatttgtg cttgttcttt tgtaaaggct
    35341 ataaggccta taacaccatg agaatcgcca tcacatataa taggacaaca cacctctgca
    35401 aactctacac aagccccact tttatagcat tccctacata tatcactact ctttgggtca
    35461 tcaattagta ttgtaatctg ttctgtaagt gattttttaa aagccgtata tttatctact
    35521 ttctcaccta ttttgtctat atatactcct gtacctgcaa tcctaaataa ctccttattt
    35581 actatagtta catctatatt gagtactgat actatagctt cagctatatt ttgaatatca
    35641 gattttatat attctaaacc tatcataatc atcattcctt atttttcttt agcataagta
    35701 tttgtcttat ttctttaaga atcaagtata tcttatttaa agtaaattgg attgacatca
    35761 ggattacaaa gagcataaca attattatta actcataatt tatttccatc taaacatctc
    35821 cttataacaa aagactaggc ttaacctagt cttttatatt atatccatat ttatatttta
    35881 actcatttat cttctttttc ccacataaat atattttaat ttatttgact ctttaatcat
    35941 accatcaaag tttaatcttg aaaatatatt aaatacttca tgtggatagt gaatctttgc
    36001 aacagaacca caaaatttat gtttaagata gtttaactta acttttaaat tctttaaatt
    36061 taaatcctct aatataacct ctccattgcc ctttaacact ctttgtattt cagatgaagc
    36121 tttttcacaa tctgtaataa cattaaattg gtcaactatt attatcttgt tgaactgatt
    36181 atcatcaaat ggtagaaact ctaacttacc tttaactaca ttgcaatttt cattatacac
    36241 tagagaataa cagacatctt cataattact attctccagt atagttacag aagttccaag
    36301 acctctcaat ctcttgccta ggtttcctat atgaccaaca agcaatattt tatcacctac
    36361 attatactca attgtatcca aaaaacattc taattcatca gatgtatatt tagctttaac
    36421 tggctgttta tttatctgac ataatatgct tttcacaatg cttcacctct tcatttcctc
    36481 aattataaat tatgtacaga ttacattata ataaatggcg agcaactatt caagagatat
    36541 ttcattagta tttttttaaa caatctttat acattttaat atgatattat accaaattta
    36601 ttaggatttt attcataaaa atcagtctat tttttatgtt tattcattgt tattttcatc
    36661 caattttttt attttcgata tggatacttc atatgccatt tttttgacca cattattttc
    36721 atctattttt ttctcatatt ctctactttg aaccctaccc caaagctgta ttctatcacc
    36781 aacctttaga ttctttgcaa atttagcatt tctaccccaa actattgatg gtatataatc
    36841 agatttatta taaggtctgt ttatagcaac taataaatcg gttatttccc ttcctaaagg
    36901 tgtcttccta tacacaggtt ccttacatac atatccatct aaaaatatac tattagggtc
    36961 tttattttcc tcgtctatac tttttatttc tctgacaaac actgtaagca ctaatctatt
    37021 tttattattt aatgttttgt tatatgatct taattggcct acgacattga ctaaattttg
    37081 tttatcaagg tcaatatctt ggagaagtct ttctgataca gtcattggaa gtatatcaaa
    37141 agaatcgctt agtctgttta tttttatttt catattataa aatttttctc caaatatctc
    37201 atggctaaat tctagcttat tgtctaactc ccctcttaag tttactacgt tattctcatc
    37261 tttaacagct atcatatttt atccctccac aattgttaat tcttatattt ctggtatcag
    37321 cttatataat aaatatattt ataagggatg tattttatta caaatctttg attgctgttt
    37381 aaaagttttt atttagttat aaatttcatt taatatttca cttttataat tataaaatta
    37441 ttatcttttt gtaccacata tactaattaa actacctatt tcagttttgt attaattata
    37501 aaaatcctat tatataatta ataagtcata tataacttaa atttattcac tctatataag
    37561 caacttcaac gaattaaatt tattgaaata attcagatga atattgagtc attataaact
    37621 tatttttctt aattataagt tatattagac ttattacagg ttatattttt attaatctgt
    37681 attaatatat gaacatttat tgaacttatt gattataaaa tttaaatatt tagctttaaa
    37741 ttatgcttta ttctttaaaa atacaacgtt ttttagatta aatctactaa atcactatat
    37801 atattacata attggcatgt taattgcttt atattaattt agtaaataaa aaactgccct
    37861 aaaatattaa ttcaatattt taaagcagtt tataatcttt attattagct gtaatattta
    37921 ataaacacta agtagtcttt aactaagcag tctttttgtt tttatttgca gccattatca
    37981 ttgtagcaga agtataagct ataactacta ttactaatgc ttttaatata tccattggta
    38041 gagtttttac tatatatgca gctacgaaaa ctcctattaa accacatata gctattataa
    38101 ctgatgcttt tttagtatat gcatcttctt ttatgaattt catacttgca actggcatta
    38161 ataatgcaca agaacccata actattggga atgcaacttt tgcagacatt cctaagaaat
    38221 aaaccattgc catacctgga gcatatagac ctataccagc tgtcattaaa gctcctagta
    38281 taaagttacc tattacacct attactaatt tagcaccagt aagacctaat tcatttcctc
    38341 cacctggtaa tacgttaatc caaggatgac ctaataacat taatattgct gttaatgcta
    38401 aagttacacc cataacaagt tgtatttttc tctcgtccat cttagctatt attcctgcac
    38461 ctatatatga accaactgct gctgccacaa ccatagatac taaagtaaca ccatctactg
    38521 ggataattga tgtaaatatg aatgcttctg taacaactgg aatagtatgt gctacgttta
    38581 atgtaccagg cattttttta tctggtatat ttaactttaa agcctttgac attgctattg
    38641 ttggagcaaa tgaccctatt cctaatgtat caaagaagtc tgttataaat cctgttacag
    38701 caacttttgg agtactttgt tcttctagtt tcccctcttt gtttgcttta gctacgtctt
    38761 taccaaacac aactgcaaag attgctgcga atactacata taatgccttt aagattgcta
    38821 ccataatcca tcctcctatt tttttatttt ttcatatatt ctgaatctta tagcctaaaa
    38881 gctatagaat agcaaccgac aagtggctac tattctattt taatttccta atttatttat
    38941 tacttattta agaataaatc catgtttaag tgggtcttct tcatctatta caaaatgatt
    39001 aaatccagtt atataagcag aaccagttat tttaggtact acagcattga aatctgcaac
    39061 tttagtttct tcaactattt cacctttgaa taaagttcct aatatacttt catatacgaa
    39121 tttttctcct acttttaatt cacctttagc atgtaaagta gctaatttag cacttgttcc
    39181 agttccacat ggagatctgt caacttgacc ttgaccaaat ataactacat ttttgtaagt
    39241 agcttctgga tgagttggct catcatatat ttcaactaag tctacagttt ttatatgagc
    39301 taaagttgga tgttgtattt ctattttctc atttattata tctctaagtt tcatagctag
    39361 ttcagttaat ttaccagcat tttgaggttc gatttttaaa cctaattgac ttgcatgtat
    39421 tatagcaaag aagcttccac caaatgatat gtcaaattta acagttccaa cacctggtaa
    39481 gtcaacttca acaccttctt tatataagaa agctggtacg tttaagaatg atacttcttt
    39541 agcttttcca tctacaactg taacgtctcc tcttattatt cctgctggag cttccataac
    39601 tacatgagtt acaggctcta ctgctggaac tacacctgtt tctatagctg ctgtcatagc
    39661 tcctattgta ccatggccac acatgttaag gtatccgcca ccatccatga agattattcc
    39721 aaagtcagca tctgggcaac aaggttgagt cattacagaa ccaaacatat cattatgtcc
    39781 tcttggctct aacattatag cagttcttaa ataatctaga ttttcctcta aatattcttt
    39841 tttctcaggc atagaatttc cttttatgtt aggtattcca cctacaacta ttctagtcgc
    39901 ttcacctgct gtatgagagt ctatagcttg tatacttctg ctaaatttca tatcaaattc
    39961 ccccttaggt ttattttgcg tctttacgca tttttaattt taggaaagcc aatgcttttt
    40021 taacatcaac ttcatccatg tgattgtctc caactacttc tgtttctatg tagcctgttg
    40081 tctttttata atcaattatg gtagctttat ccaaatatgg attggtaatt actaatccat
    40141 ctgctcccat aatagtaaga cctactgtag gtatatctga ttttacaaac tctgctatag
    40201 catttgtaaa atcaatatgc tggtttcctg ccatatctaa agttataatc actccatcag
    40261 gtcgcaatga ttcaatccat cttccaagtc tagttgctaa aaatgttttc cattcattgt
    40321 gctctgatgt cccaactaca accactccta acaagtctat gtcagaatca ttatctatca
    40381 aatctagtaa cgggtctcta aatatatgaa gtgatgtgtt tttcattgat gtagaaattc
    40441 ccacaaaact cattccctta cttaaatttt ttatccatgt tttctcattt taagttttaa
    40501 aaatgccaaa gcttttttag catctatttc atttgtgttg ttttctccaa ctacttctgt
    40561 ctcaatacct tcttttgatt tgttcatatc aactatggta tccatatatt gattgcttac
    40621 aacaaactta gcttgagtcc cattaaatgt aactccaact acaggtatat ctctttttcc
    40681 tatttcctca aatgtgttag cgtagtcaac atgtgagttt ccccaaccat ctgatgaaac
    40741 tatagctcca tctactctca tggcttccag ccaagcggcc gttctttgtc ccacaaaggt
    40801 tttattttca tttccatctg gagttcctac tactattact ccaagtaaat ctacatcctt
    40861 atcactttca actatgtcta aaagtgggtc tctaaagtga tgaagagatg tttcttttgt
    40921 tgaaggtcct attcccataa tatccacctc tcttaagtca tagctctaag ggcaccatct
    40981 ctgtattcat taggagatat aatcataggt acatttccca tatctataat tgatgttcct
    41041 ccttcaaatc cactaggttc ctttgagaat aactgattgt catacatagc accttgacct
    41101 gctatttgtt ttactatgac aacctttttt gcatttggtc gaattttgtc aaaatattca
    41161 cgaacttgag tagcatttct tccgtcttcc attttcaaag acgctcttat ttcttgtata
    41221 aaatcatcac atgctctgaa tgctgcaagt ggaagtgttc tttcaaaagg taaacctccc
    41281 tttaaagtta catctacatg gattatataa tcttcaactg atggagttcc atatctccca
    41341 aataccattt ggtctttcaa aattccttct gaagaaccaa attcatgcat ttgatttcca
    41401 tcttcatctg caccagttaa cattacatat acacccgtta aagtatgtgt aataccctct
    41461 cccagtctgc ctaaaacttt tgttgatatt ggtataatgt ccataatagt attaatttct
    41521 ctgtcataat ctccaggttt tataatgtct acttttatgt ctgttattaa ctcttctctt
    41581 tcttttattt catctatact tgataaatct attgtaagta catctttctt tatagaagtt
    41641 tttgaaccga aattaacttc attcatatga aacggtttta taacaagtct tctcattatt
    41701 ttttcttcca taatatccac ctttgttctt aaaacccctc tcctaaccgt gtaggagaag
    41761 ggttctatat tttcaaatat aaattacatt ttaaacgtga gctttatatt cgtatggtaa
    41821 tggaactatc ttaccagcag atggtattgc aactaattgc tctaatgtat ctcttaatat
    41881 atgtgtttgc atttctatat tatgtggttc accagcatta gcacccattg gaactaatgg
    41941 agcaactgct ctaggagttc cgttttgtct aactactgga ggaagagctg ctattattat
    42001 tgtaggtatt ccagcttcct ctattgctct ctgcacgatc acggcagttc tatggcaagt
    42061 acctcagcca gctgtcataa ctacaccgtc tactccctct tccttaagtt tagcagcgat
    42121 agcaggacca gtttcttcag tgaagacatg ttggtttcct ccaccaccca tgaatgcata
    42181 gtgcatagga gctactcctt tgataaatcc ttctgcagct aattcatgta atctatctat
    42241 agggaacata cagtttatat ctttgtttac atctccattg tcatatccac catgtgatac
    42301 catcatttca tcaactgtag ctgtattagg tatagctcta aatgtagtat ctcctgctag
    42361 attaaatctc ttgtcagatt ttaaatgaac accagctgct gtagctaaag ctatagtcat
    42421 atcttttaat tctttagtta caggagtcca aactggagga ggtgttattg gaacgaatat
    42481 ttcagattga agtccttgta ctgttgtaag gctcatatat ttcctctccc ttctattttc
    42541 aaacgattat ttattatttt tcttcttgtt ttgattgcat ttctgcttgt ttttcttttt
    42601 ctctcctaat attttctaca tactcttgat taggctctgg acttaatacc tcaggatagc
    42661 ttagcatgaa tccaacttct tgacctatct taagttcgta gtcagttata agcattctat
    42721 taggtatttt aaattgtcca agtctacctt ttaggtctat agtcactcca ccgcctttga
    42781 tgtcaactat aataccttca taaaaagtct ctgatgagat atattttaac ctatccatta
    42841 taattctacc tctctatact gttttaaact tggatctaaa tttatgttat tgtctaaatc
    42901 ttagttagta tctaaatttt agttttcgtc tttttcgtat atttctcttc tcttcttact
    42961 cattggtaga gattgctcat tctcttctaa tactattttt tctcctgtaa ctttttctat
    43021 agcttctatg ttatttaatt taacatttgg attccatttt ctttcaggag ctttaacttc
    43081 tacaccttct atagcatttt taagcatagc cattattcta acagcatctt ctggagctaa
    43141 agtgttgtta gataatattt cattctctat accttgctta gacttattgt tgtctaccat
    43201 gtgagtcatg tatttattac caacaactag agcaccttga acagctgaga aacttacacc
    43261 aactactggt atacctctca tacctatttg ctctatgtga gatgcgaaat ctatatggtt
    43321 gtttccgaaa ccttcagtag ttactacagc tccatcaact tccatagctt caactaacat
    43381 acctaatctc ttagatacat agaatttctc agcatttact tgtggagaac caacaaatac
    43441 aacacctact aaatctattt cttcgtcttc catagctctt attactaatg gctctctcca
    43501 gtaatgtctt gacatttctt ttgatgcagg tcctatacaa gttaatgcat ggataccacc
    43561 atctaatact tcaagtggag ataatacaac tggcatgttt cctaagtcaa cgtttggttg
    43621 agctcctaat gtaccaactg gctcaactgg taatattagg ttatcatgca ttgcaccttg
    43681 tcccattatt tcttttataa caacaacttt ctttttacct tctcttctgt attgctctat
    43741 tacttcttca tcaactacta aagagttgtc aactttcttt aatgcttctc ttatttcttg
    43801 agttacatag tcaaatgcac ggtgagcagc taaaggtcct ggtctttcca tgttagttcc
    43861 tgcttttatt gttacttgac ctttgatgaa tatttcacct ttgtcagcag cacctggtct
    43921 accccacatt atagtagtat ttaactcacc ttctgaagaa ccaaattcac ctatttgaac
    43981 tccatcttca tctgtaccag ttaataccat tacagctcca tctataactc tagttatacc
    44041 tgaacctaat tcgccttctt ctttagtagc gattggttgt atatctaata cagcttcact
    44101 gtaagtttca tatttatcag gagttattat ttctaacttc atatctacaa ctaactcttg
    44161 agtttcatta gcttctttac atatttcttc tgcgtttctg atgtataaag tagttccttc
    44221 tattttagtt tcatcagcaa attcaacttt atctatttta aagtgttttt tagttaagct
    44281 tcttaaaagt ttcttttctt caacttttgg agctacttct gctgctgcac ttgccactgg
    44341 agcagcaacg tttgctactg gagcagcaac tcctgcttga ccagctactg ataaaggtat
    44401 ctctaagctt atgttcttac cttcacctat gtgtatttta actgtttcac ctgcaactcc
    44461 tgtgcttact ggaactactg ctgcttctga tactggagct gcttcttcta ctgtttcttc
    44521 tactacttct tctttagcct ctcctgcttt gtatccttct acgttatctg tagtcattgg
    44581 agaaagtgca tctaaagttt cttttaactt agctcctagt acttgaccta tagttaatcc
    44641 attttctggt attgttaata atccagaatc ctctaagtct gcaaatattg ctggatcttc
    44701 taagttttct ggcgctataa ttgttcccgc ttcaaatcta caacaacaaa ctgctgggtc
    44761 atttgcatgg gcttgagctg tttctaaagt tattgacata gtataaaata cctccttaca
    44821 ttattttctt atttattatt ttaaatctca attcctcaac aggtaacccc ttaactgaga
    44881 aattaagtaa attaaaacct acataatttg ttatgattaa tattattttt ttctcactta
    44941 aagtcacttt atattataag caattcctat gccaacttat taacaaaaag attttttcta
    45001 taatagattc ttcatctatc gctattttta gccaaatatt atataaatat ttatttaaaa
    45061 tatgttattt tttgtatttg taaacatttt ctcaaacaaa aaaagtgtca attaatcaac
    45121 actttttcat tttttttaac acatctatat acacaacact tatataagcg ttggctttac
    45181 taggttttaa attaaagttt atcattttgt caaaatttag tcatgtgtca tattttagac
    45241 acatctattt tgtattggtc tatcttataa tacaaagttg ttctgggtat atttaatatc
    45301 ttagctgctt tagctttatt tccattagac attttaagtg ccttggttat attctttatt
    45361 tcaatttttt gagttgcctt cgttaaatct aaaggatact cttcttcttc agtactatta
    45421 tttgtagaat ccatcatata acttggtaca tcatccacat ctattttaga attttgtgac
    45481 aatacaacaa tattttcaat cgtatttttt aattctctaa tattaccttt ccatctataa
    45541 ttctgaaata tatctattac atctttgcta attgtaagaa caggcttatt attttgttta
    45601 catatctcct ctaaaaagct atgaaccaat aatcctatgt cttcttttct ctctctaagt
    45661 ggaggaatct ttatttcaac aacatttaat ctatagtata aatcttctct aaatttttct
    45721 gcctttacca ttttactcaa atctttattg gtagctgata ttattctagg atttattttt
    45781 attgtggtat ctccaccaac tcttcttatt tctttttcct gtaatactct aagcaacttt
    45841 gcttgcatac taagaggtaa atcggcaatt tcatctaaaa atacagttcc atcttttgca
    45901 agttcaaata tacctattcg acccttttta cttgctcctg taaacgcacc tgattcatac
    45961 ccaaaaaact cactttcaaa taactcattt ggtatggcac tacagttaac aggtataaat
    46021 tgaccttttc tctcactata atcatgaata gctcttgcaa atacttcttt acctgtacca
    46081 ctttcgcccc aaataaatat gctactattt gttctagcca cttgttttgc aatcgctttc
    46141 gatttttcca atttataact tttacctaaa atcttatcaa aactaccttt agataaattt
    46201 ttgacttcgt tctttagata ttttaatgta tcatttactt tttcaagttc aagagctaat
    46261 atcttagctt ctgtaacatc aacttctgta catacaacac cgataaattc ttctttataa
    46321 aatataggat ttgcatgaac taatgcatac atattttctt ttttatctgt aaaatacaag
    46381 tcactcattc caactttact atttagtact ttttctgaaa tagtattttc caaaaattca
    46441 ttcatcggtc gaccaactat atcttcagac tttatatcat acctttcctc cataaactta
    46501 ttccacaaga taacaacacc ttcgttattt atagcacaaa ttccttcttt aatttgacct
    46561 ataatatact tcaaagttaa tccataatct tctaaatgca tatatagata gtccctaatg
    46621 tgctcttgtt ttagaactcc aataatttta ttatctctaa gaacaggaag aatccctata
    46681 ttttttaata tcattatatc tctacattca tctaaggtta aaccttcgtt gacatatatt
    46741 acatccttct tcattataag ttttactggt tgaccttcat actttttatg catctcataa
    46801 agattatgta tatccgtcat ggatataatg ccttttaatt ggtcagatga atctattacc
    46861 attagagttt ttgtgttgct ttttatcatt tcttttattg catcttcaat tcttgtgtct
    46921 tcgtctatag ttgtaaattt tgtatccatt acctcttcta ctttttttac ttctggtatc
    46981 gaaaacataa agtcccccct ttatagtagt acctcatcaa aacttattta tatatattta
    47041 ttctagcata ttataataat tatttattaa ataattatta ttaaaattta ttaagtaatt
    47101 agcatttaaa tttatataat cactttaaaa tttataatta aatttataca aatgaaatca
    47161 aatgaattat acccttagta tattattttt tcaatactat tttcaccttc aaaacatttt
    47221 tctattcatc aaacaattaa tgaatgcctg tatccaatca taaatttaag aatataatta
    47281 aaatatatat ttttaaacaa actatatttg catattatat ttacataaaa aagccccaaa
    47341 atgacttata aatcaatttg agacttttta ttttaaactt aataataatc tagattaatt
    47401 tctttttatt ttacattgat acgctaatcc acaaattgca atgacactaa taaatacata
    47461 tgacattagc tgagtttcat cacctgtttt tggtgatttt attattgtat catctcttga
    47521 tagttctgga gttttttcca ctgtatcact tactgatggt actggtggat ttaacactgt
    47581 atcctctgtt ggtggcactg gtggatttag cactgcatcc tccgttggtg gcactggtgg
    47641 atttaacact gcatcctccg ttggtggtac tggtggattt attattgtat catccattgg
    47701 tggtactggt ggatttatta ctgtatcatc tattggtggt gatggttttg gcttatgtcc
    47761 atttgtatca tctgttggtg gcactggtgg atttacaaca tcatctgttg gtagacctat
    47821 attaaagtta acttcttgac catttttttt ctctattgta taacttaata aattttgctt
    47881 actagatggt aatgaagatg aatggtcttt ttgtcctaaa tcaagcactg ccttataata
    47941 ttgaatagct tttggaaaaa tataatcata agatacagca gaaatattag ctttggcatc
    48001 tggttttcca acactcttaa gtttaaattt ctgttgcgtt gaaatttcta cttcgcctgt
    48061 acctattata ttaccatcta catcataagc agttactcca tctggtagtt ttaactttac
    48121 tacaccacta taacctacta aggctacagc ttctgtttca taagtaccat cttcattttt
    48181 ttcaaagtta atcttagtta aaccattact caaactaggt tttcctgtat catttggctt
    48241 taactcttca cgacctgctt ttaacaatgc ttctaaataa gcttttgtat ttatcgctac
    48301 ttctttcctc ttctgatact cagctctttc ctcttcagtt tttgctattt ctataagttt
    48361 atcacaaagg tctatagagc cttgatactg cttaagtctt tcttgttcat ctatttgtcc
    48421 aagaataatc caaattgcac tttgagtagc tgagtaagtc attatgtcat tttcttcttc
    48481 gcctaaatta aattgttgct tatatccaaa tccatcaaat tcagaacctg catatagtat
    48541 agatttaact gcttcttcat tttggttttc ttgaccatta aaatcttctg ctggtaaata
    48601 tggactcttt ccattactac ttggtggtct taaatgatta ttgtaacaaa atgcagtttg
    48661 ctctccatct tcaccttcta ttacatattt gtcgtctttt gagcttgctc ctacaagacc
    48721 ctttattaca tagtcaaaag ttaaagtctt tgtaatatca tcaaatctta aatcatgata
    48781 aggtctatct gaaccatcta tttcttttag taaacaagaa tttttcacct ttgctttttt
    48841 gtcataccat aagctaataa atgagtcttc tgaagtaaaa acttggttaa tttctgattg
    48901 ttcatctgcc tttacatctt cttcatcttc ccaaaagtta tatactattt tatttatttg
    48961 agataaagtc atatcaagaa ttttatcaat atcttgtttt aaactcattt cctctataac
    49021 tatttcttct tccaatggtt tttcttcttc tggaatttgc tcatctaatg gtttctcccc
    49081 atctggcatc tgaccatctg atggtttctc tacatctggc gtctgactat ctgatggctt
    49141 ctctccatct ggcatctgac catctgatgg tttctcccca tctggtatct gaccatctga
    49201 tggtttctcc ccatctggta tctgaccatc tgatggtttc tctccatctg gtatctgacc
    49261 atctgatggt ttctctccat ctggtatctg accatctaat ggtttctccc catctggtac
    49321 ctgaccgtct gatggtttct ctacatctgg tgtctgacca tttgattgtt cttctccatt
    49381 aagtattgta tcattgctag tcacttcgtc agcataagat actattggtg taaatgcagt
    49441 aaataccata ctaaaagcaa ttactaaact gattatactt gattttttaa tttttgtttt
    49501 caaaattctc ctgatactaa aaacatatat tgtattcact taatataatt aaaattatta
    49561 aatattatat gaatcttatt caataataca tctttagtgt gttcaattag ggtcgttact
    49621 cctaaccagt tctcctatga acttcttaat atttctatta agcacagact atatcattac
    49681 ctaaccataa attaggtact ccccatttcc ccacacttgt aggtacgaga tttctctcta
    49741 gtcgttgaag tttcttcttt tcgaagctta cctgctgatt atccattttc aaggcactta
    49801 ggatttaacc ttatgctatc taactaattt tttctacttt cgtaacctca agtgcttatt
    49861 aaatgcacac tctatcacac ttatccttat ttcatgatta tgttgtggtt tagttagctt
    49921 taggaacttc cagcaattaa aggagttttg ggcagatatc tgccactcta cactcgttat
    49981 gaatgcaggg agcatttcaa aaatatattt atatctatct tctattaatt acttatttat
    50041 gtatttattt tatatatttt tgacaattaa aagttactcc tatttatatt tatagcaggt
    50101 tgcactacta actccatata tcttttaagt gcccaggttt aagatatact tcacagtttc
    50161 tataaatagg ttttttaatt tttctttttt aactttttaa gtgcactttt ttaacaactc
    50221 aataaaatat aaattagtat cctagatact tatctaataa aagacaaaaa ttagttttta
    50281 taataaaaaa gatactatag agtatcttca aatatcacaa aaacctttta ctctatcgga
    50341 tagttgactt aatataatat gcatattaat acaaaccaca tacttgagta tcattatttt
    50401 tgtccaatat taattgtaat agctattgtt atcattatac aatatctagc atattttgtc
    50461 tattttttcc atttatttta caatatatat tgccctcaaa atacattaaa aaacatcttt
    50521 agaagtaact tttaagtttt acttctaaag atgtctaact tctatttatc tattatttta
    50581 tttttaatct tacgtcacct tttattcttc ttattaatgg gtgattaact gttctcatta
    50641 catggtcagt aatatgatat acacctaatt ttaattttgg tgcagaaccc attactgtgt
    50701 tactacagtc agtacagttt cctattaata taacttctgc tccagctttc ttaaattcaa
    50761 tacatttttg agcttgtcct ggacagtgtc ctggattttc acattttaat gcacctttaa
    50821 tttcttgagc ttgtttacat ttttgaactg atactatatc agtaactccc attttagttg
    50881 ctatatctct cattctttct tcattaggtc cacatgcaca aactaaaaga cccatttttc
    50941 tcttttcatt aacaaatggc atagtaacta ttataccacc actagtttta gttattggag
    51001 catctaaagt agtagcttta ccagtgaaag gtcctcctag gattatttca ccatattcac
    51061 cttttattcc accagctctt tcaattaatt ctccgacagt tgttccaatt ggcacatctt
    51121 caaacactat agattctttt ccactgttta attgaccaac tacagttata tttttagata
    51181 taactggtct tttttgctca acagcctcta ctattctagt tagtgtttct acgtttatta
    51241 ctactgcatt agcctctgaa ggtaattgag ttggctcaag taattttcct agaacgtctc
    51301 ttactattgc tctctcttct cccattgggt acatatcaac taattctgca acttctatgt
    51361 tgtctcctgg ctttattact tttttgaaag cttctattgc ttttggatgc ttacttttta
    51421 ttgctagcat accttttcca gcattagtaa cttccatagc atattttata ccttgataaa
    51481 ctatttctgg tctttcttct atttctttta tattatgagc taataaaggt tcacattcag
    51541 ctgcatttgc taatattgtt ccaccattta aatctatgtt taattttaca tgagtaggga
    51601 atcctgctcc acccattcct actactccag cttctttaat taagtcaagt ataccttctc
    51661 cttttaatgg ttcaaaagta tcttcttgta cttcatcagc ttcaattact atcgcttggt
    51721 catttatttc acttattttt ccactaacac tggcataaat atttgcccct aatccagttg
    51781 gttctgcgat tagagttcct ttttttacta catctccaac acttactact ggcttatctg
    51841 gaccaccaac gtgttgtttt aataaaatag aatatagttt gttcaactag tacgccccct
    51901 taagtatatt aattttttta taagctgttc aaataatgtt aatgctattt ttctattata
    51961 aggtcttaac atattgttta tttctataga acatatcgct tattaacaaa gcaatttcta
    52021 tgccaacttt taaacaaact tatacccttt ggaaatactg tcttacagca ttcattaatt
    52081 cttctaaatt tttaaaccca tcaaaaaaaa gtcaattatt agacactttt ttaatcaatt
    52141 taatctataa ttattgatat tgcatacatg tataaaaagt gtcaaaaaat agacaccgtc
    52201 tttttatcga cacttttcta acatatacta tataatttat cacaacttta cttaaaatat
    52261 acatttatta cttattaatt cttagttttt tgcactccat tggaatcaac tacataatta
    52321 tctttataca acaaatcaga tattttgact agactgtatc catctttttt tagtcgactt
    52381 attatagtat ccaaatagtt atctacatca tttatattag catggaataa aactattgaa
    52441 ccaggttgag attcttttat tactttttca attacatgat ttggtccaag ctccttccag
    52501 tctatagaat ctacatccca cttaattact ttataaccta aatcactaca aatgtccata
    52561 gctttattgt ttacgtcccc aaaaggaggt ctaaataaat tagtcttttt tcctgttata
    52621 ttatatattt tttctgatgt agtttctatc tcttttgtta tgtcttcttt tgatatttcc
    52681 ttcaaattgg catgagtgtt tgaatgattt ccaatttcat gtcctttttc atctatagct
    52741 tttacaattt cttcattatc atccacccaa ctaccaacta aaaagaaggt agcttttaca
    52801 ttatgtttat ctaaaatttt taaaatatca tccatattat cagttcccca tgctacatca
    52861 aaactcaaag ctattttttt ctcatttgta tcaactttat atataggcat ttttgaattg
    52921 tcagatttag aacatatgta ttttattcca taaacaaaaa caaatattaa cactaataat
    52981 aatgaaaaaa ataaaacact atatatcaaa gctttagtcc ttttaacttc tcttttcata
    53041 ctattccccc tttgaaataa tttcttctat atttaaaatt tatttttttt cagctcaaaa
    53101 aagtactgta atatttaatt tataagacaa taactaaaaa aataaaacct atattgacct
    53161 aattaaactc tctaaaatac tcatttattt aattttttta tattttttac atttttacct
    53221 ttatttttct gtatatatta tttttatttt ggagaaatta gtattaacat cacataaata
    53281 aaagtgttgt tagacatcat aaactaatta tttgaggagg aaatttacta tgtcaaattc
    53341 aaacagaaca gtagttccag aagcaaaagc tgctttaaac caaatgaaat tagaaatagc
    53401 taatgaaata ggtttatcta actatgaaaa catagacaaa ggtaacttaa cagctagaga
    53461 aaatggatat gttggtggat atatgactaa gaaattagtt gaaatggctg aaagacaaat
    53521 ggctggaaaa taaatagtaa attttgacac ctgggtatca acagggctgc taattagcag
    53581 ccctgtttct ctatatttat ttataaaatt gtttttatat gaattattaa agattaataa
    53641 tacaatacaa attgttcttt taattttgta caaattagtt taatatatta tatatagaaa
    53701 tagtttgacg gaggatgata tttatgtttg aaaactcgtc tgaagaatta gcctatcaca
    53761 aacttttaat tttatatata ttagaaaaaa ttaaaatgga tttaactaat tctcaaataa
    53821 ctcaagtagt attagagact gagatgatga attatttttc attacaacag cttttatctc
    53881 agcttatgga atctaagttt ttgactacat ataaagactc tgacagagaa tattatgctc
    53941 ttactcaaag aggcgtagaa agtctagaat acttctttaa tcgaatacct actagtgtta
    54001 ctgaaaaaat agacaagtat atagattcta acaaagaaaa tctactagca gatactcaag
    54061 taaagtctag ttttgtaaaa caaagtgata atgaatttat tgtcaacctt agagtcatag
    54121 aaaatcagtc taacttaata gatttaaatc taaatgtttc atcagagaaa caagcaaaac
    54181 ttatttgcaa taactggaaa aataatgcat cttatatgta tgccgaagtt atcgatttac
    54241 ttataagaga catacattaa agcctacaaa caaattatag ttgtaggctc ttttcctacc
    54301 ttgatttctt ttattaattt gttatttgca gtttcataaa cttttactga attttcttgt
    54361 gtatctgata caaatagtaa cttttcatct ttagatattt ctaatccttg gggaatccca
    54421 tttatttcaa tatttccaat gtatctactc gttgtataat ctaaaattct tacattatta
    54481 taacccaaat cagcaacata aacttttttg ccactataat taaatcttat ttctactggt
    54541 aataaaaatt catataatac cttttttata tccatgctta cttcatctat tattataagc
    54601 tttccatcaa gtgttgatat atacatttct ttcttttgtt tatcaagttc tatatgccaa
    54661 gctctaaagt ccatatttat tttcttttgt atatttttat taattgtatc tatacatact
    54721 atcgagttta tacacggtac atataacttt ttgctttctc tatcaaagtc aaatccatgt
    54781 ggcatattat caactccaat aattcctatt ggatttaatg tgtttttatc tagtacatat
    54841 atactattag aatcttcatt tgccacaaac aattcattat cgctaagtac aacttgacta
    54901 agacttcctc ctatagagac agtatcaatt attttatcat tacttaaatc taagacatag
    54961 agtatcccat tacttgaact aggtatgtat attaaatttt tttctttttc tatgcaaaaa
    55021 tggtgtggat atatattatc ttctaatact atttcttttt ctagttcaag tgtagaataa
    55081 tcaactatgc taatactctt tgataagtag ttagaaatat agattttcat ataacccctc
    55141 ctctctacaa tacatatact acaataatat attatttcat tagcttgtat tatgctttat
    55201 agttgcatta ttacgcatat atttggtatt attattaatg ttaaaaaaag aaataatatt
    55261 taatggaggt ctttttaatg aaagtaacat ttaatccaag tggtgtatgt tgtagagaaa
    55321 tgatttttga agttgatgaa aataatgtga ttgttgatgc tgagtttata ggaggatgta
    55381 atggtaattt atcaggactt agaagcttaa taattggcca aaatgctctt gaagttgctg
    55441 ataaattaaa tggtattgac tgtggtggaa aaggtacttc ttgcccagac caattatcta
    55501 aagctatacg tcaatctata taattactta tataaacatc agtaaaatta ttaaaaactt
    55561 attttatata aaaagagttg tccatttttt agacaactct tttttatatt tttatttgta
    55621 tacttatttt ataaaattat aatttcttta ctaaagttct cactgtacca atcatatata
    55681 atgaacctgc acttattata atatcatcct cacttgatgt ttctaatgta taatttactg
    55741 catcctctat ctcatgcttt gatgttacat catctacata ttttaatact ttttctctta
    55801 atatatctga atttatggcc cttgggttat ttggtgttgt tgttattacc ttattaaaat
    55861 gtggcaacaa tatctctaaa acactatcaa tatctttatc ttctaacata cctattaata
    55921 aggttaattt tctaccttta aaattcttat caagtgcttt tgccaaagat tttgctccat
    55981 cttcattatg tgcaccatct attataaaaa ttggattttc ttttatcttt tctattcttc
    56041 ctggccattt agtatttata agtccttttc ttatagattc ttcatttata tttgctaatt
    56101 tatttatgtc ttttagatat tttattacac taattgccaa gatagaattg tttatttgat
    56161 gttcgcctat caattttatt tcaaggtctc tataagtttc tttcattaca gtacaatcgt
    56221 atatttgaga atttatatct gattttttta tgtttatatc atcaaaatca acttctatat
    56281 atttagcttt tttctcttta caaactgact ttataacatc ctttgcttca tcagtctggt
    56341 catatactag tactactcca ttttccttta ttattccagc cttttcgtac gctatttttt
    56401 ctattgtatc tccaagtata cctgtatggt ccaaacttat agatactatc acacttacaa
    56461 gtgattttgt tattatattt gtagcatcat atctgccacc taaaccaact tctaatgcca
    56521 caaagtctac cttttgttct gaatagtaat aaaatgccat tgcagtaacc acttcaaatt
    56581 ctgtcggata tgcatagcct tcttttacca taatctctat tttttctttt attaactcaa
    56641 ttattctagc aacatcatcc tgtggtatat tttgtccatt tactcttata cgctcagtaa
    56701 aagtttctaa atatggagat gtatataaac caactttata tccacactct cttagtatat
    56761 tagaaacaaa agaacacaca gaaccttttc cattagttcc agctacatgt attatattca
    56821 aagtttcttg tggatttcct aataattcta ataactttcc tatattttct aatcctaatc
    56881 ttataccgaa tttattagtt tgtgatatgt actctaatgc ttcttcatat ttcattatct
    56941 aactccccct gtattgtcat tttctcttat gctatctaat tatagtatat tttttacttt
    57001 taatctaccg acaaagaata catataattt aataccttct acattaaatt tagctacaaa
    57061 tctttattta taagttttta tatctttcca ctaattcagt cagtgtatat attgaaacta
    57121 agtcatattt tgagatgtcc atataattaa actttatgtc aaatctatgc tctagctttt
    57181 tcaacagatt aaaacaatta tcataaacca agcctatctc cttattaaaa aggctaccct
    57241 taaagagttt ttcattattc tcttcaatat ctaagcaatc cctaacaacg tcagcaactt
    57301 tcataaaaag agcattttct ttgtcataca aatccaattt aaagtttaca attctactac
    57361 actcatcaaa atcacaacta tcccaatgat ttctagttgc taatgattca agcttatcat
    57421 ttaaatcaga tattgttatg cctggttttt ctgataatat cttagctatt ttaccagtaa
    57481 gctttgctga taaataacta ttacattttg gtggtaatct ataggaatta tttatatcac
    57541 aacttatata tgaattgcta tccataacac attgaacatc atatttttta ttataccaaa
    57601 atgaatcatc tccatctaaa gtaaatcctc ttacacctat aacattgtta aacatagctg
    57661 gataacttaa gtcaaaatca ttagcgagtg aacatactat tatttttcct tttctaaaca
    57721 attcataaca aacttcaaat aagcccttaa cactttctac tcctatgact gataaactta
    57781 aattaattaa tcttatgtta gtgtctaaaa ggtatcttaa tgcttcttca agaactttta
    57841 tatttgtaat accatctttt ctcaaaattt ttattacaaa taactctaca tcttcatact
    57901 ctttctttat tattgatgca caagcagttc catgaccatc ttcatcatca aatttatctg
    57961 atataaagat ataatcatgc atacattcaa aagcaattcc tccaactaaa ttatctttta
    58021 agtaatcatg ttctttgtct attcctgtat ctaatactgc aaccttaact ttattattca
    58081 tatcacatat attctccatt acaaaagttt ttaatatatc tatgttaata gttcatctta
    58141 aactttgatt taagttgcac aatttatctt ttaaatgcta tatttcttgc taaaaacata
    58201 aaataagtac accatatatt taatttatat ttattatatc aattattatc attaaatggt
    58261 taaatgttaa gataaaattt ttagatttat atcacaattt agaattattt gtaaaaaagg
    58321 agattatctt tttattagac aatccccttg tatattttat taatatttat ctatgattaa
    58381 gcatcaattt tattttatac ttttttattt taatactata catctaaatc ttatttaggg
    58441 ggaacaatta tttatatgct ataaaaatgc taatttgaac gaacttgtct tataatcagt
    58501 tttagaaaat acaccattct acaaagtatg aatcaaaagg tgaaaaaata ctaaaaataa
    58561 taaatcaaca tcaattttaa tcaatatatg aaaaacttat accaagtatg cataaaaact
    58621 aatataaata ttattttaac aactaatact tacaccttta tatattatat catatttcaa
    58681 taaataaatt gcataaaagg aaatcttttc atagaattaa actatttatt aattcattac
    58741 tataggttca ataaactata agatgtcgct ttaatattta tattgtactc cattaaatat
    58801 taatataatt aattaaagta tgcatagcta aatgaaacaa atatctatag agttttataa
    58861 atagtcatta ttttaaaaag gtaatcatat tctaaaaata catataatac atataaaaat
    58921 caataatagt atattgataa tcaagctact aattaataat catgctatta taatggaaac
    58981 tctacatata catgttaaac catattaaaa atctataaac tgctattttg cataataaaa
    59041 ctgtcttaat ataaataaca ttttatatat taagacagtt tcaatcaata gccctgtgat
    59101 gacattaaat cttttttacg atattttcaa ttaccttttt aaaattaata cattatatca
    59161 cataacaagc ttgttaatgc tacttttaaa tctttaagtc tatacttact tataggtata
    59221 aattgttcat taatagtaat catattatcc ctaagatctt ctattcttct taaattaaca
    59281 ataaaacttg tatgacatct aaaaaagcca ttttcacaca gtaatttttc tgcttgtttc
    59341 atgctcatat tagctttata atctctacct attgtatgta cgactaaatc tctctttaca
    59401 gtttcaacat ataaaataga atctatcagt attctattaa atcctttttt actatttaaa
    59461 actatataac gctccttctt tctttgaagg ttatcaatac aaagatttac acccattgca
    59521 aagttactat actctatagg ctttactaaa tatctataag cacgaacctc ataaccttgg
    59581 ggggcatact ctgaaaaagc cgttgtaaat ataatttcta ctttttcatc aaactttctt
    59641 atttttttag ccacctcaat tccattgata tctttcattt ggatatctaa aaatataata
    59701 tcaatttttt ctgggtattt ggatattaaa tcttctccac aggaaaattc cagtatttga
    59761 taatttagac atctttcaga caaaagtttt tctgtaaact cttttagtat gcttctttgt
    59821 attttttcgt catcacatat aactaccctg aacataatat cacctccaaa tctaaaatat
    59881 catttagttt actttatttc aatttccaaa ctgtactcac tagctttcaa atttaaattt
    59941 ctttataaat ttaaattgtc gattaaacta cttatagaaa ttctctttaa tatttaaact
    60001 taaattacga taaattccta tacttttcta gatatattat gtaaaaaaat atagtaaaat
    60061 aaatataata tgtatttatt accctatatt aaacatcata atatatcaaa tttttattgc
    60121 ttataaattt taagacataa aattcatata ttcacaacat aaaataaaaa tataacacat
    60181 ctgtaaagaa ttataacatc atatttgtat ata
//
LOCUS       DAGSRM010000014        56922 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.014, whole genome shotgun sequence.
ACCESSION   DAGSRM010000014 DAGSRM010000000
VERSION     DAGSRM010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 56922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56922
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.014"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(285..1124)
                     /locus_tag="MJE33_000967"
     CDS             complement(285..1124)
                     /locus_tag="MJE33_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBY9646932.1"
                     /translation="MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YTSGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMFILIVVGAITGIL
                     GLF"
     gene            complement(1148..1906)
                     /locus_tag="MJE33_000968"
     CDS             complement(1148..1906)
                     /locus_tag="MJE33_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBY9646933.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNDNVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            complement(1953..2426)
                     /locus_tag="MJE33_000969"
     CDS             complement(1953..2426)
                     /locus_tag="MJE33_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY9646934.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIVIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSYNGDENLIVLVKYPKTVLGLVNSGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELISKDIKCFIQIVPDAKKIDVGTLL"
     gene            complement(2455..2871)
                     /locus_tag="MJE33_000970"
     CDS             complement(2455..2871)
                     /locus_tag="MJE33_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY9646935.1"
                     /translation="MIHMAGIIIATHGNMAKAMLESAELIVGKQENVETLGLNHGDSI
                     DEFSRKLEDGIEKYKDEGVLVLLDFYGGTPFNTSAIIINKFAKVCELECLTGVNLPML
                     LELFLNRDTMKLKDLKNLCEEVGISGIKDVKTVLKL"
     gene            complement(2918..3970)
                     /locus_tag="MJE33_000971"
     CDS             complement(2918..3970)
                     /locus_tag="MJE33_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBY9646936.1"
                     /translation="MKSVRFYGIRDTRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGKEVRSFKIGDRVAVCPAMPCFECNECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGPIGLFSIQWAKIFGSTKVIAVDVFDEKLDLAKGLGADICINAKEKNI
                     VEEIKRLTDGDGADIVIESAGTPLTCGQVLLLAKKGGTVLYAGVPYGDVALTREQFEK
                     IVRSELTVKGTWFGNSFPFPGKEWSAGLYHMQKGDMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIDN"
     gene            4366..5046
                     /locus_tag="MJE33_000972"
     CDS             4366..5046
                     /locus_tag="MJE33_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBY9646937.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSVKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            complement(5179..5499)
                     /gene="sugE"
                     /locus_tag="MJE33_000973"
     CDS             complement(5179..5499)
                     /gene="sugE"
                     /locus_tag="MJE33_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBY9646938.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     gene            5881..6708
                     /locus_tag="MJE33_000974"
     CDS             5881..6708
                     /locus_tag="MJE33_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBY9646939.1"
                     /translation="MNFYKIAVCQMITTENKIENINHAVDMVTEAAINGAKIVVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEDGKIYNTCYVFDK
                     NGDLIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGIAICYDIRFPE
                     LSRLMALKGAEIVILPAAFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGRIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
     gene            7530..8204
                     /locus_tag="MJE33_000975"
     CDS             7530..8204
                     /locus_tag="MJE33_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY9646940.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNSYEVYTAQSGKDALKKISK
                     NPDIILLDINMPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGADDYIVKP
                     FDLDELGARVSAHLRREQRKSNQTKIKFYNDLTIDYEKRLICIYNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETVWGV
                     GYKWIK"
     gene            8192..9352
                     /locus_tag="MJE33_000976"
     CDS             8192..9352
                     /locus_tag="MJE33_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBY9646941.1"
                     /translation="MDKINNMSLKKAFFVLTLSSLIIASVLTTFAYILLNNLYNSIQD
                     KYLKTPINTGIITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYK
                     VKLKTPIEILNKGAMEISNNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNIKMWTM
                     INDRKQLNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSDHINRIE
                     YYVENMSNAQRLEDLVVSKSMSNINKFIENLDENISIIAKQEGKSFTLKSQINNINLF
                     FDENIILRVVENIISNAFRYARNNVSILIYLEQELLTFVIEDDGIGFSKESLKLALRP
                     FYRDKTLNDSNAHFGMGLYISKILCEKHGGSISIENNSTNSAKITAKFSTRN"
     gene            9623..10096
                     /locus_tag="MJE33_000977"
     CDS             9623..10096
                     /locus_tag="MJE33_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBY9646942.1"
                     /translation="MLSSTFCSLPFWICYFWCGISDIFDGLIARKLRQQSGIGAKLDS
                     IADFIFSVTILVTVGKNIALPMYLWWCIVAIALLRLISYSIGFFKYRTFSSLHTLMNK
                     ITGISIFAFPLLYICFGLDVAGIMICIVAFLSSFEEIAIMIKSKELNRDIKSIFM"
     gene            10382..11050
                     /locus_tag="MJE33_000978"
     CDS             10382..11050
                     /locus_tag="MJE33_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9646943.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLEINSGEFVSVIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHTEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPDKEYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            11053..13521
                     /locus_tag="MJE33_000979"
     CDS             11053..13521
                     /locus_tag="MJE33_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9646944.1"
                     /translation="MKNYLSLSIKELKSQKLMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHVSFHQLTKNQVDDIKKDDRVYQAGTSITLGSSKIKDSGISVLV
                     KEYDKTGLSNYPKLMKLKSGNLPKDKNEIALDENTLKLMGIKPRLGVTIPMNLDISLL
                     NDTIPPYNYTANFKLSGILEDDYTGYVSGIVNGIVGKGTSENLLPKRYILSSLDFKIK
                     QQEKFQEIVNQLAKKINLSHNSIQYNWIYLNALKIQFEKDENSSNSDGISMIILVSLF
                     VALLVLLASGLVIYNILKISVTKKIKEYGCLRAIGAEPNQIYKIVILQILILCTVAIP
                     IGAVIGIISSKGITGMVTNILNPDILLANDNKEITELIHKNTTAYMFPLVLSTNVSLI
                     FSFISALPSAIYASHVSPKIAMVGSTTKIKRKIKREKTIKNFERHLAWLNLKRNKGRT
                     IITILSLFMSITVFVALSEFSNVLDVSRSVSNLKEGDFSLTNEISGFDKSTLDKITKM
                     KNVNRTSFIKYSEYKQGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITDSILEDFK
                     NGSICFIKNPIAISTPGVKTRHTNLKPKDSITINKKQLDIYSTVDKMFFLQGNGWING
                     VDVIVYDSVYNTLTNKNKFNQINIYAKDKSKLEQIRLSIEQICENNPGSHWISYIESD
                     KQLKESFKQIEFLAWAVILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNSSLY
                     KMFLWEGVYYGIFAAIFGSIAGYASAIIINMATIEKLDFTNIPITSILQATIISVLAC
                     IIATLIPLRKVKKMNIIDCIDINL"
     gene            complement(13761..15185)
                     /locus_tag="MJE33_000980"
     CDS             complement(13761..15185)
                     /locus_tag="MJE33_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY9646945.1"
                     /translation="MKNQSLITQFRHTFIFIIIASIVATVITYVFALYLYIHSLNKDI
                     YPPNYYERQVPRIEKYINEKNITLLSQSNEEGLKRTIRGDDMLYQVVDNNGNILYGTN
                     PKKLFKTKEELFNNFINKTVRKGGYIHTVPIKGDNGKIEGAVILFYQVKITFANIRGR
                     FVFAVIIMALFSPFLYIVGFTRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYY
                     SDNELGKLCSAFSEMKDELKGSLSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDAL
                     IGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQLNLVPINLPEFLRQ
                     KVQDYELQAHQKEVELILKMQGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISI
                     TVKDEKNCISYEICDSGRGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVE
                     QLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(15204..15896)
                     /locus_tag="MJE33_000981"
     CDS             complement(15204..15896)
                     /locus_tag="MJE33_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY9646946.1"
                     /translation="MSKILIIDDEMDLVMLLEDELKAKGHEVLIAYDGQAGIELSKQE
                     PDLIILDIMMPKMNGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFGKLCLYMKERAIKIDGKNIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            complement(15951..16727)
                     /locus_tag="MJE33_000982"
     CDS             complement(15951..16727)
                     /locus_tag="MJE33_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBY9646947.1"
                     /translation="MIKLLSSEWLRAKRTAVQWLTLCMPIIFSLCVVFYLTTKSGSTQ
                     EFAFEGFFTVWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMSIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGLTSVGEKVWAFVPWTWPVKLGMLPGIYFIKEAGTISTEA
                     FYSKVMQTASIELIVVAIGLIIFLIGGVIWFKMWEGRKSY"
     gene            complement(16724..17494)
                     /locus_tag="MJE33_000983"
     CDS             complement(16724..17494)
                     /locus_tag="MJE33_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBY9646948.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QTTSWAWQNLLALIFNWWSFLFLPIGFALFATLVAFQEKKAGNYRALRTHNVSPMTLW
                     INKVIAMAVYSFISTLVLIVVTIITGLILKAGPVPFGQIIGASIVCWVVSLAILPLQL
                     WLATWKGMFLSMGVGALGMIFGVLAATKPFWIAVPWSWAVRMVCPIINIHPNGTILEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            complement(17491..18195)
                     /locus_tag="MJE33_000984"
     CDS             complement(17491..18195)
                     /locus_tag="MJE33_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBY9646949.1"
                     /translation="MKDLILETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTNGRIIFDRKPWKRESLAKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLSDPELLILDEPTNGL
                     DPFGIQELRELIASFPKKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDPNEN
                     LEEFFTDVILKGGQNR"
     gene            complement(18450..18962)
                     /locus_tag="MJE33_000985"
     CDS             complement(18450..18962)
                     /locus_tag="MJE33_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646950.1"
                     /translation="MKKISQEKISTHIQEIPQEKIEQWKSLYEKYKSILKVNSKSVLE
                     IIEFLKRKYVIVEETSEEFKHVVINNIKLNKPFSQKISSDKELKPIVFTIPNEGNAKK
                     LYDTQEEIYSGCKIIVGMEFETGCVFVEGSDELNDEITSFKGLDKYDINNYYLVANYI
                     RCLKKYKLID"
     gene            19288..19545
                     /locus_tag="MJE33_000986"
     CDS             19288..19545
                     /locus_tag="MJE33_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646951.1"
                     /translation="MILNRAELFRGYSLNRFNEICNILSVNKIKYKYKVKNNAYSKKP
                     FFNEITLGNLGQKEDFSYEYLIYVHKDDYEQAESLINSKLR"
     gene            19688..20314
                     /locus_tag="MJE33_000987"
     CDS             19688..20314
                     /locus_tag="MJE33_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HBY9646952.1"
                     /translation="MKINIKSKLSEFIKQNNLFDDSRIISYLPNITIETDKIKTIMIN
                     EVVPTNTNDDFYSVDKDADYLKTTIPLFDSAGIKVSNINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTY
                     KLRNNELYYDNLRVFPSYIMTGGNILIEKSKFEMASEDIKKMFEIING"
     gene            complement(20561..21340)
                     /locus_tag="MJE33_000988"
     CDS             complement(20561..21340)
                     /locus_tag="MJE33_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901940.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBY9646953.1"
                     /translation="MNSKIYYFSGTGNSLVVSNILKETLNGSKEVIPLAIHRKENNID
                     INEDILVIVFPVYFADVPDIVKSFVEKLNFNINTHIYAIATCNGVAGHSLFTIDKLLK
                     KRGKKLSAGFLINMPGNALITPPDVEEERLKNQTNRVAEIAKCINNLEIGNIEGKNNL
                     KSHIDSVVLRLVGKNMQIATTKFFAENKCNGCGTCKRVCPVENISIVDKKPKWGNECE
                     RCLACFHWCPKEAINVKKSFLTNRKKYHHPDITVKDIFLDK"
     gene            complement(21408..21869)
                     /locus_tag="MJE33_000989"
     CDS             complement(21408..21869)
                     /locus_tag="MJE33_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBY9646954.1"
                     /translation="MGGARDYLNGGKMKDFEKLYEATTKLVSTSLKVKDDLKKMFKQN
                     GYDITTDHYALLRFLWEQDGISQIDLCEKSCKDKSNTTRILDVMKNKGLIVRKVDVKD
                     RRKFQIFLTDLGRELEEPLNEIASIYATETFKSISDEELPLFTDILDRIGK"
     gene            complement(21845..22522)
                     /locus_tag="MJE33_000990"
     CDS             complement(21845..22522)
                     /locus_tag="MJE33_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBY9646955.1"
                     /translation="MIITVINGSPRKNGATSKVLTYLYKDIERLIPDVKINYFDLSEV
                     NPSYCIGCLNCYKMGKCINQNDKVEYIHDIITKSDGVIFGSPTYGSSVTGLFKVFTDR
                     AHMMLERLLYRKPCIAVTTYENARGSKAISFIKSMVLDSGGYVCGSLSIKTGFNQNPI
                     TEKVESKIQKVSKKFIYCIEEKKNPPVLSQIYNFIAINAVLKPMAFKDIEQYKGIIDR
                     WEEQGII"
     gene            22755..23141
                     /locus_tag="MJE33_000991"
     CDS             22755..23141
                     /locus_tag="MJE33_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBY9646956.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYAPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYVARESSSFLNRFYNGTLNSMVLNFL
                     ENDKLSEDDIEELREILNKRTTKGDK"
     gene            23144..24943
                     /locus_tag="MJE33_000992"
     CDS             23144..24943
                     /locus_tag="MJE33_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HBY9646957.1"
                     /translation="MYAPFFIRFLLSTLTLSILIVVILLAKKVFKKHISAKYQYNIGF
                     ILLIMLIIPFVPLKYINLGNVFNYLYTFNQLGSSTNRTSMIKNSPNSVINNSNALNDF
                     SVSLNQVNFDSLNILLLISWIIGILIMIAITIHCNIKIKNMKKSIQILRDDEIVFIFE
                     KCKKDIGINKDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLKDIKYILLHELQH
                     FKNKDILINYIMCFLQILYWFNPLVWYAFKEMRIDREIACDISVLKKLDKDSHIEYGQ
                     TLINFVDNISRPSNLTLTSDIGGSKKQIKKRLLKIMDFHNESNTLKFKSILIFTLIAL
                     IVFGNIPKLSIMADINEKYRPTNEQTVYEDLSQYFKGFDGSFVMYDLKSKQYHIYNKE
                     KSKTRISPNSTYKIYSALLGLENGIITRNNSHIPWDGKHHSNSSWNEDQDLFSAMENS
                     VNWYFQTLDKKTGLKNIQTYLRQIGYGNYDVSGGISNFWLESSLKISPIEQVELLESF
                     YTNKFKFEDKNIETVKDSLLISKKDGVSLFGKTGTANINGKDVNGWFIGYVEKNENTY
                     FFATNIQSKDYSNGSSASKITLSILNDKKIYNP"
     gene            complement(25298..27172)
                     /locus_tag="MJE33_000993"
     CDS             complement(25298..27172)
                     /locus_tag="MJE33_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBY9646958.1"
                     /translation="MNKYNKIANELIKIIGEDNIISITHCATRLRVMVKDREIINDKK
                     VEKVDEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLLGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEIEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIDLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLVSIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVVSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDVA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(27177..28841)
                     /locus_tag="MJE33_000994"
     CDS             complement(27177..28841)
                     /locus_tag="MJE33_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBY9646959.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKDLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKESKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDEGYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDTTPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSEYSDCLIYGSIEPIDIESEDVIAYRRQLDLVVIDCY
                     YNFSDKPVEIRVEEKYDIVFQNLDDINLLNKTVCLKPFHAVLLKAR"
     gene            complement(28855..29676)
                     /locus_tag="MJE33_000995"
     CDS             complement(28855..29676)
                     /locus_tag="MJE33_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBY9646960.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKKREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDIHKIC
                     LWSNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDIVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            complement(29775..30539)
                     /locus_tag="MJE33_000996"
     CDS             complement(29775..30539)
                     /locus_tag="MJE33_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBY9646961.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVIDDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVAKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDASKIIDFAKEMRMKGIYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            complement(30536..31285)
                     /locus_tag="MJE33_000997"
     CDS             complement(30536..31285)
                     /locus_tag="MJE33_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBY9646962.1"
                     /translation="MRLESLVNQNYDNLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KSDLFIIISLSGETKAGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            complement(31789..32394)
                     /locus_tag="MJE33_000998"
     CDS             complement(31789..32394)
                     /locus_tag="MJE33_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBY9646963.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGENIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEDVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMMKSISELDNLKYEWICMSHGTPCIK"
     gene            32573..33211
                     /locus_tag="MJE33_000999"
     CDS             32573..33211
                     /locus_tag="MJE33_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY9646964.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQGMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVTGKSYLNNFNIDEEKFIETTLALLIDSIKA"
     gene            33343..34005
                     /locus_tag="MJE33_001000"
     CDS             33343..34005
                     /locus_tag="MJE33_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY9646965.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCENISLNKVEVKAYLDNVEVPVTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DN"
     gene            34005..35186
                     /locus_tag="MJE33_001001"
     CDS             34005..35186
                     /locus_tag="MJE33_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY9646966.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVVYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTIHAENCHNGGARFIIKFY
                     T"
     gene            complement(35367..38027)
                     /locus_tag="MJE33_001002"
     CDS             complement(35367..38027)
                     /locus_tag="MJE33_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9646967.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIKLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYVNSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKNIASSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQDASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(38024..38707)
                     /locus_tag="MJE33_001003"
     CDS             complement(38024..38707)
                     /locus_tag="MJE33_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9646968.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEIASLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            complement(39051..40004)
                     /gene="blaCDD"
                     /locus_tag="MJE33_001004"
     CDS             complement(39051..40004)
                     /gene="blaCDD"
                     /locus_tag="MJE33_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HBY9646969.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYLGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNDKNKKEKINMVDYSDCFEDISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLINKVDAKYVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            complement(40545..42659)
                     /locus_tag="MJE33_001005"
     CDS             complement(40545..42659)
                     /locus_tag="MJE33_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HBY9646970.1"
                     /translation="MSENKQVVNCDIIASNSKHNMCEYDAELLTFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDAKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKINKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     SERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPKVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMATKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHVVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMKQLYNDGELVMLNKSYRSTFEIINFAKKIQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKIEIEEFKKSDNATLGIILKTDNDAKAVYDAL
                     REKYSVHLISSESSSFTKGVSITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYTGELTQLIDM"
     gene            complement(42743..43579)
                     /locus_tag="MJE33_001006"
     CDS             complement(42743..43579)
                     /locus_tag="MJE33_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY9646971.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIVANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLAFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     LS"
     gene            complement(44174..44536)
                     /locus_tag="MJE33_001007"
     CDS             complement(44174..44536)
                     /locus_tag="MJE33_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBY9646972.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDDALKPFIETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQD"
     gene            complement(44770..45234)
                     /locus_tag="MJE33_001008"
     CDS             complement(44770..45234)
                     /locus_tag="MJE33_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBY9646973.1"
                     /translation="MTRISNITIAEQSEYCFLAIRKTIDFMVEFSEFSKQSFEKISKY
                     LEERGILSSGAPIVCFHNMDLAKLDVEVGFPVATYIDGKNEILQRIVPAQKIITAIDL
                     GPYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            complement(45663..47084)
                     /gene="nhaC"
                     /locus_tag="MJE33_001009"
     CDS             complement(45663..47084)
                     /gene="nhaC"
                     /locus_tag="MJE33_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBY9646974.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNIFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPIGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VVFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLRLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKIARIEDEPETCLNFDV"
     gene            complement(47114..48178)
                     /locus_tag="MJE33_001010"
     CDS             complement(47114..48178)
                     /locus_tag="MJE33_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HBY9646975.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERNIKISFVTKSFCAQKEIV
                     EEICSEGIDHIADSRIQNLKNLQDINLPKILIRIPMLSELEEVINYCDISFNSELGTI
                     KKLNELCESKNIIHKIVLMFDLGDLREGYFYEEDFFSNVREIVRLKNIEIIGIATNLT
                     CYGAIIPSRENIGRLVSIAKRMEEKFGINLEIVSGGNSSSIHLLINNNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGVDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDNLEFLL
                     TYGGIMSSSTSKYVSKKIIV"
     gene            complement(48163..49572)
                     /locus_tag="MJE33_001011"
     CDS             complement(48163..49572)
                     /locus_tag="MJE33_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HBY9646976.1"
                     /translation="MENSKVSLKIDVLVAEIGSTTTVVNAFHDINTNNPIFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDDIKYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQITSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNVENQEEIRLIFEDTNYKLYITENVYPKIDLLNIEPTRRIIQSVF
                     EEHITTAPGMKYIKEMVNENITPTPGAVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELSIDIDAVIENY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKVKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIEILDNMLKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            complement(49572..51776)
                     /locus_tag="MJE33_001012"
     CDS             complement(49572..51776)
                     /locus_tag="MJE33_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBY9646977.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYK
                     DCTKPLKKSIGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLKIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELRDKARELKERAVLYMEEISEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYDENAIDNPSMLIDGCTFENPDKIIYIDELDDFDNVENRLKESYEYRNGTKIKPEME
                     WCADGIVMITMMLPTDKRTAEFAALEFVKKMNLQEIEVISREVMHESEGTRIEVKGRV
                     PFDIDLNNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMSNK"
     gene            complement(51770..52144)
                     /locus_tag="MJE33_001013"
     CDS             complement(51770..52144)
                     /locus_tag="MJE33_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HBY9646978.1"
                     /translation="MKKREDDFEVRRKHLQELSDDELKERFWSLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVKGEYWDEVTCLFREGVESC"
     gene            complement(52222..53652)
                     /locus_tag="MJE33_001014"
     CDS             complement(52222..53652)
                     /locus_tag="MJE33_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HBY9646979.1"
                     /translation="MNNSTSMKDMSYQAVMGRNNEIMKNAIGLDYSSFEQEGIGFDYE
                     KMMSETGYTLQDIEAIQSQYAVGNTPLIELKNLTKLARKCAKEGKGARIFVKDEAMNA
                     SGSFKARRAATAVYHAKQMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNG
                     VGQPEIIEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVET
                     LGYELAIQFREKYKKDPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLH
                     MASDNQFNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTT
                     ELLAQLEGIERGPAGNTSLAAAFSLAQELDEDKAIVVQETEYTGAGKHICPQLTFARE
                     NGIDIKFGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKL
                     SNRDIEFLMEEVKSSRDFVVDVLNNL"
     gene            complement(53645..53953)
                     /locus_tag="MJE33_001015"
     CDS             complement(53645..53953)
                     /locus_tag="MJE33_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-ketopentanoate thiolase"
                     /protein_id="HBY9646980.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGDLVEVKTITGRLVKGNLLKINPYYTHDYGKCIPELLQIGIQAKEILFGGVYNE
                     "
     gene            complement(54074..55141)
                     /locus_tag="MJE33_001016"
     CDS             complement(54074..55141)
                     /locus_tag="MJE33_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-binding protein"
                     /protein_id="HBY9646981.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     NVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKIIFLLNRKINVIS
                     TAEQMAYPQADDADLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMVEQGVGVTREEFIKGVEDKTIAGHVGFVESINMIADGLGWKL
                     DKIEQTKEPIMTTVDRKSKYGEALAGNVAGCRQCGYGYVNGEVLIEMEHPQQIIPEAE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            complement(55397..56770)
                     /locus_tag="MJE33_001017"
     CDS             complement(55397..56770)
                     /locus_tag="MJE33_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY9646982.1"
                     /translation="MNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILTNSF
                     FDIVNKMKINRSTLLEVLEKREVIKDNIQKYLDKNGKEITAINTTIPIDIKGEFVGAL
                     EISKNMTTIENLLNRNYKKNLVNKVSSRQKKGYEFDDILGESNLIKEAIAKSKKACKS
                     DASVFIYGETGTGKELFSQSIHYGSNRKNYPFIAQNCAALPESLFEGILFGTSKGGFT
                     GAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRII
                     ATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDIPLLCKKFISKYNKKLNK
                     KVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYNLKEN
                     KESNNYSLNDLEDKTLSKIISEIEEKYIYESLEKASYNITKAASILGMNRQNLQYKIK
                     KYNIKIL"
BASE COUNT        19302 a         9676 c         6749 g        21195 t
ORIGIN      
        1 tttaatatat aactaatatg ttataatatt ttaattaaat aaatttatat ttttttgaaa
       61 ttacttaata ttttatataa aaatgaaaaa gcacctcatt ctgattaaaa atctttctga
      121 gatacttttt aaaatattaa atactacata tagcttacat aagtttaaaa tttaatctat
      181 tataaaatta ccctaattgc caaacttttt aatataaatt ttccaacatc ctacaaatat
      241 aatcaataga tatagaatcc taacttacac caatatatat ttatttaaaa tagtcctaaa
      301 atacctgtaa tagctcccac tactatcagt ataaacatta tttttattgg agaaagacct
      361 ttttttaaag caaagagtac taataaagta agtaccaaag gaattaatcc tggaaatatc
      421 ttatcaagta aatctgtttg aagactaaat ttaaaatcac cactagtata acttattggt
      481 gtagataaat tgataaacct acctacaagc ccccccatga ctaaggtacc tagtatagaa
      541 gccccttcta taaccttatt tactattcca ccttgaagta tactttcaac agcttttttc
      601 ccatacttat atccatttgt ccacattcca aaagaaattg aagtcattat tatatattga
      661 gctattacaa ataatatagg tcctgcaatt gatgctcctc cctcagttaa accaatacaa
      721 acagccaaca ctataggagt tataactcct tgagttaaag tatcgccaat tcccgctagt
      781 ggacccatca agcctgtttt gatagcattc ataccatctc cacttatttc atctgctcca
      841 ttagcttttt cttcttccat agctattgtt attccatgta taacacatcc acatataggt
      901 tctgtgttaa aaaatgctag atgagtctcc acttcttgtt ttaactcttc cttatctgga
      961 tataattttt taagtactgg caacattgaa tgtgaaaaag ctgttgattg caatctttca
     1021 taattgtaat tagattgtgc aaagaaaaac catcttaacc atgattttat tacatctttc
     1081 ttgcttaacg ttttcttaga tacttcattt tttatatctg acataatatt ttccccctca
     1141 catgtattta agatgtagca ctatcttttc cgtacttaat tgaagtaatt gtaagagcaa
     1201 gtattactcc aataacagat attactatta tatctacttt aaaataaaca gccattagta
     1261 ttcctaatat gaaaaagggc atagtaaact tgtttccaat caattttaaa ttcatagcaa
     1321 tcccaagtgc tggcaacata gccccaatta cattaaatac atgtaacaca ttatcattca
     1381 ttacttccaa cataccatct atcgcatttg gaccaaaata aacaactaat aatgctggta
     1441 taaaactcat tgcaaataac agtatctgtg gtggtactat atttataaat gctactctat
     1501 caacattccc tttttgaacc tctctatcag cccaatgtgc aaaaaaagaa tttactgtca
     1561 tctttccaat ccaaagtaca gttgcaacag ttcccaatgg tacagctatt gccattgcag
     1621 tacttacatc tagcttagat gctatagcta aagcagtacc tacatatcct gcaaaagctg
     1681 ggtcccccat ctgagctccc cctgctgata taaaacctag atatatcata tttatacctg
     1741 ctcctataat tgctccttga acagggtctc ctaatataat tcccgttaca aatccagcca
     1801 atagaggtct attccatgta aaaaatccaa cataagtcaa ccacggactc caagaaagat
     1861 aataaaaaat tgctattaat actgcttgaa ttagtgaaat gtgcatattc ttcctcccta
     1921 ttttctttta aagatttaca agatagtctt ctttataata atgttcctac atcaattttc
     1981 tttgcatctg gtactatttg tataaagcat ttaatatctt ttgatattag ctctctaaat
     2041 atttcttttt cttcatctga cacagatata tttttataaa aactttttct accaagccct
     2101 gctcccatac ctccaacatt caattccttt aaatctaccc cgctatttac taaacctaag
     2161 acagttttag ggtatttgac caatactatt aaattttcat ctccattata tgattttaag
     2221 acttcagcac ctgattctac atcatgtact tcaaccttaa cagttgatgg agctgccatt
     2281 tgtaaaactt gaactataaa tgggtccttt actactaaat catccacgat tactatcctt
     2341 tttgctgatg taatcttaca ccaagcagta ataacttgcc catgtattaa tctatcatca
     2401 attcttgtta atgctatatt actcataatt attcctccag atttttaatt tattttacaa
     2461 ctttaaaaca gttttaacat ctttgattcc tgatatccca acttcctcac ataaattctt
     2521 caagtctttt aacttcatag tatctctgtt taaaaataac tctaaaagca taggtaaatt
     2581 tacacctgtc aaacattcta actcacatac cttagcaaat ttatttatta taatagcaga
     2641 tgtattaaat ggagttccgc cataaaaatc aagcaatact aaaacccctt catccttata
     2701 tttctcaatt ccatcttcta actttctact aaactcatct atgctgtcac catggtttaa
     2761 tcctaaagtt tctacatttt cctgtttgcc tactattaat tcagcacttt ctaacattgc
     2821 tttagccata tttccatgtg tggctataat tattcctgcc atatgaatca tacctttctt
     2881 aacctttttt atatggatta ttatttagta ctctaatcta attatctata ttaatcatta
     2941 tcttaccaaa gaaaatatcc cttttgtata ctttttcaaa ataagctggt acttcttcta
     3001 aatttattct atgtgttact aatttttcaa cattcatatc acccttttgc atatgataca
     3061 atccagcact ccattctttt cctgggaatg gaaaggagtt tccaaaccaa gttcccttta
     3121 cagttaattc acttcttact atcttttcaa attgctctct agtcaaagct acatccccat
     3181 atggtacacc tgcatacaat actgtacctc cctttttagc tagcaacagt acttgaccac
     3241 atgtcagtgg agtaccagcc gattcaataa caatatctgc accatctcca tcagttaacc
     3301 tttttatttc ttctacaata ttcttttctt ttgcatttat acaaatatct gcccctaacc
     3361 cttttgccaa gtctaacttc tcatcaaata catccacagc tattactttt gtagaaccaa
     3421 atatttttgc ccattgtatt gaaaataatc ctattggacc tgtaccaagt actacaacag
     3481 tatctccaac tttagcttct gacctaaaaa gaccatgtcc tgctatacat actggttcta
     3541 gcgcagcggc agtctcataa cttatctcat ctggtatttt tattaaattt ctctctttca
     3601 cctttgtata ctctgcaaaa caacctccaa gttctttatt tcctattatt gctacattat
     3661 tacatcttga atacaatcct tttttacatt cattacattc aaaacatggc atggctggac
     3721 aaacagcaac tctatctcct attttaaaac tccttacttc tttaccaacc tgtgccactt
     3781 ctccagaaaa ttcatgccct aaaatttctc caaccatatg tggacctgtc tttgaatatt
     3841 ttgatatatc agaaccacat attccagcta cttttacttt aattattaca tcatcttttt
     3901 ctaatatttt tggtacatcc acatcctcca ctcttgtgtc tcttatacca tagaatctaa
     3961 ctgacttcac tattaaccac ccctactttt tatttgagaa tatatcccta ttgtttatat
     4021 gttaaatatc taaagcaact ttcatgccaa tatatgtaag cattgtattc atgcatttta
     4081 taaaattatc attaaatgtg tgtcaaagtg tgtcaaaagt ttcttatatt aaaacttatg
     4141 tgtgtcaata taaacgacac taacatttta tattagttct cttattataa gttttataaa
     4201 atgtactttt tttgttattt ttataaaatt gttcggaaat tccccttcta tttgtgatat
     4261 aatattaaat ataaggtatc aatataaatt ttcatgcgaa tatcagaact tttttgacac
     4321 taatgcctta ttcaatataa taatttaact ttttggaggt tgtttatgaa aaaagtgtat
     4381 caaggaaaga ctaaagatgt ttttgaatta gataatggaa attacttact taaatttaaa
     4441 gacgatgtaa ctggtaaaga tggagtattt gacccaggcg aaaatagtgt aggtctaagt
     4501 atagatggaa ttggtagagc taatttagag acatctgtta agttctttga aattctaaac
     4561 aatgctggaa ttaaaactca ttatgtaagt gctaatttag aagaagcaac tatggaagtt
     4621 ctaccagcaa aagtttttgg taatggatta gaagttattt gtcgttatag agctgttggt
     4681 agtttcttac gtcgttatgg ttcatgtgtt gaagaaggtg ctaaactaga ttgttacgta
     4741 gaagcaactt taaaagatga tgaccgttgt gaccctctta taacatctga aggtttagaa
     4801 gctttaaaca ttatgactca agctcaattt gattcaatga aaagcatgac tcaaaaaatt
     4861 tctaacatag ttcgtgatac tatagctgaa aaaggtcttg agctttatga tattaaattt
     4921 gaatttggat tctacaatga tgaagtaatc ttaatagatg aaatagcatc tggaaatatg
     4981 cgtgtttata aagatggtgt aatagttgac cctatggatt taactgctct tttattagat
     5041 aaatagtata tacatataat ttacaaatat aataaactta taaataagat aagctatgaa
     5101 gtaaagactt tttaaatgtt gattattttt aaacattttc tcgcctttaa tttcatagct
     5161 tatttaaatt aatatactct aattagtaga taatagcttt aaccctacaa ttcccaccac
     5221 aataaaacct atacatgcaa ttcttgatat actaataggc tcatttaaaa tagccatgcc
     5281 tacaatagct gtaccgacgg taccaattcc agtccatatt gcatatgaag ttccaagtgg
     5341 aagatgtttt agtgctaatg acaaaaaata aaaacttaca atcattccaa caacagtaag
     5401 tatacttgga gttaatttag aaaatccatg tgaatttttt agttgaaaag cccaaaacac
     5461 ttcaaacaaa cctgcaacaa acaaaaataa ccatttcata aattcatccc tcttttcaaa
     5521 tatgtctctt atttttccaa taaaataacc tgagaaatat tataaaatat ctccaaggtt
     5581 tttgtccttc cgtgttcaca acaaaaacta tgttgtgggt tttatctcgg accagaccaa
     5641 tcattaataa ttgcggaacc ctataaaact ctattaatta ttctatttta tctataataa
     5701 tactatcaac atatataaaa aaagtcaata tatgggttcc atatataata aaaataactt
     5761 ttatatacct tttaatattc ttattcactt ctctccatac ccatttgggt caatctaaga
     5821 atatctaatt tttatttaat atgttataat aacataaaaa tattgaaagg agtaatttct
     5881 atgaatttct ataaaattgc agtatgtcag atgattacta cagaaaataa aattgaaaat
     5941 atcaatcatg ctgtagatat ggtaacagaa gctgccatca atggagctaa aattgtagtt
     6001 ttgccagaaa tgtttaactg tccgtatgaa aataaatatt ttcccaaatt tgctgaagaa
     6061 tatccaggtg aaacaacaac gatattaagt aaattagctg agaaacatgg aatttatcta
     6121 gtttctggta gtattcctga gttagaagat ggaaaaatct ataatacttg ctatgtattt
     6181 gataaaaatg gagatctaat tggaaaacat cgtaaaatgc acttatttga cattgaagtt
     6241 acaggaaaag ttagttttaa agaatcagat actcttacag ctggaaatga tgtcactgta
     6301 atagataccg aatatggaaa agttggtata gctatttgct atgatattcg tttccctgag
     6361 ttatcacgtt taatggctct taaaggtgct gaaatcgtta tcttacctgc tgcatttaat
     6421 atgactactg gtcccgccca ttgggagtta tctataagaa tgcgtgcttt agataatcag
     6481 atattctatg ttggtgctgc acctgctaga aatatgaatg cttcttatat agcttttgga
     6541 aattcaagaa tttcagaccc ttggggaaga ataattgctc aagctgatga aaaagaatgt
     6601 attatttatg ctgacattga tagagattta attcctgaca taagacaaca gttaccactt
     6661 cttaagcaca gaagaactga tttatatgaa cttaatactt tgaaataaaa cagaacataa
     6721 ccccataaat cataaattaa gtgttaaaaa aatttatggg gttctcatta atttgattta
     6781 aaattaattt cataaaaata atgctaaaat taatttaatt aaataatact aataattact
     6841 aatgattatg ctaattatca gcatttacta atttaatcac tattagttat ttcttttatt
     6901 aaatatcttt acttttttac ttattatgtc ttctagcttt acatttggat tatggtattt
     6961 ctttctattt ttagtcacat ctttctattg aattacttgc ttaaaacacc aatgcacaca
     7021 tgccagataa tgttcacaat tatgtagaaa tcttgctcta ccttcttcaa tgttgatatt
     7081 atctctccta gttgatatta tctttcctaa aatttctttt aattaaaaaa taacattagt
     7141 tgatatatca agtatatttt aatcaactga aaaaaacaat aataattgat atatcaacta
     7201 attaatgtag attaattcat ttttataatt tatatacttt ttaattaatc taccttattt
     7261 aattaaaatc cttcctttct atttatccat tttcaactta aattataaga tttcatcagc
     7321 tatatttttg cataccaata attattaaat atcaaatata tattttatta aaatttctaa
     7381 taatttcatc cttgatttat ttaatatata aaaatatact aactttaatg attaagtttt
     7441 gaaatataac taagaatttg ataatttatt gagattttta tactaaaata tacatactat
     7501 acaaacttaa tttagaaggg agcattctta tgaacaataa aatactcgtc gtagatgatg
     7561 aggaaggtat aataacactc ttaaaagatt actttgaaat gaatagttat gaggtttaca
     7621 ctgctcaaag tggaaaagac gcattaaaaa aaatatccaa aaatccagat atcattctac
     7681 ttgatataaa catgcctgaa atagatggaa ttgaagtttg taatagaatt agagattatg
     7741 tatcatgccc tattctattt cttacagcta gaattgaaaa tactgacaag ataaatggtt
     7801 ttcgtgctgg tgctgatgat tatattgtaa aaccttttga tttagacgaa cttggagcta
     7861 gagtatctgc ccatttaaga cgtgaacaaa ggaagtcaaa tcaaactaaa attaaatttt
     7921 ataatgattt aacaatagat tacgaaaaaa ggttaatttg tatatataat aatcagattt
     7981 cactttcaaa aaaggaattt gatatagtag agcttctctc tatgaatcct ggacaagtat
     8041 ttgataaaga aaaaatatat gatagagtct ggggatacaa tagcaatgga aatagtgatg
     8101 ttataatgga acacattaga aaaatacgaa tcaaactatc aaaacatact cttgaaaatt
     8161 atatagaaac agtctgggga gttggttata aatggataaa ataaataata tgtcattaaa
     8221 aaaagctttc tttgttctta ctttatcttc actaattata gcatcagtgc ttactacttt
     8281 cgcatacatt ttacttaata atctatataa ttctatacaa gataagtatt taaaaacacc
     8341 tataaataca ggtattataa ccatagttca aaacaatgtg aaccaattta gtgattatga
     8401 taaaacatta cttaatataa tcaacatact tcagcttgta cttccactta tattttttat
     8461 aggactttta ttattagctg atataatttt ttacaaggta aaattaaaaa cacccataga
     8521 aatactcaat aaaggtgcaa tggaaatatc caacaacaat ttagactttt gcttagagta
     8581 taataataat gatgaattag gtaatctatg taatgctttt gaaaaaatga ggtctgaact
     8641 aaataaaaac aacataaaga tgtggactat gattaatgat agaaaacaac ttaatgctgc
     8701 tttttctcat gatttaagaa atcccctaac agttctaaaa ggttactcaa actatttaac
     8761 aaaatacata ccaacaggta agcttagtga tgagaagatt ttatcaacca cacaacttat
     8821 gtctgatcat attaacagaa ttgaatacta tgtagaaaat atgagtaatg ctcaacgatt
     8881 agaagattta gttgtttcaa aatctatgtc gaatataaac aagtttatag aaaacttaga
     8941 tgaaaatata tctatcattg ctaaacaaga aggaaaatct tttacactta aaagtcaaat
     9001 aaataatata aatctatttt ttgatgaaaa tatcattctt agagtcgtcg aaaatataat
     9061 atcgaatgct tttagatatg ctagaaataa tgtatccata cttatatatc ttgaacaaga
     9121 actccttacc tttgtaattg aagatgatgg tataggattt agtaaggaat ctctaaaatt
     9181 agctttaaga cctttctata gagataagac tttaaatgac agcaatgcac actttggtat
     9241 gggtctttat atctcaaaaa tattatgcga aaaacatggt ggctctattt ctattgaaaa
     9301 taattctact aacagtgcta aaattactgc taaattctca acaagaaatt aaaatgattt
     9361 tgtaagcttt cactgttctt atataaaatt tgttagttta gaatttataa aatgtatttc
     9421 aatttacaac caatatatat actattttgt agaagttgta agagttgctt caattataat
     9481 attttggcaa aaggcatttg aaacaataat agaaatatat atttaaatat gtagaatagt
     9541 atgaaattag aattttggag gtgattatat taaaagtttt gctaataata ttacattctc
     9601 tcgtattgtg tttgcattgc taatgcttag ttctacattc tgttcactgc ctttctggat
     9661 atgttatttt tggtgtggaa ttagtgatat atttgatggt cttattgccc gaaagttgag
     9721 acaacaaagt ggaataggtg caaagttaga tagtattgct gatttcattt tttcggttac
     9781 aattttggtt actgtcggaa aaaatattgc tcttcccatg tatctatggt ggtgtattgt
     9841 tgcaatagca ttgttacggt tgattagtta cagtattggt ttttttaagt atcgcacatt
     9901 ttcatcgctt catactttga tgaataaaat cactggaatt tctatctttg cgtttccatt
     9961 actctatatt tgctttggtc ttgatgttgc aggaattatg atatgtatcg tggcttttct
    10021 ttcttctttt gaagaaatag caattatgat aaaatcaaaa gaattaaatc gtgatataaa
    10081 aagtatattt atgtgaaaac ttagaatttg tgacaaaaat aatttatata ataatgagga
    10141 agtaaaacat aaaaaattat ccacaagttt atgtgtaaaa tagttttaaa gcaatagagt
    10201 tgtattgtaa agcctttagt gcataaatta cattttaaat taaaaatgat actgaaactg
    10261 cttatcagca ctgcgaatta tccgcaaata gtgaagactt tttagattta agctaaaagt
    10321 tgggaaaaaa ttgagtttta aaacttacac tcttcttata tagaaaagga gagtgttttt
    10381 tatgattata aaagcaaaac aattatcaaa aatatatggt tctaataaca ataaagttat
    10441 cgcacttaat aatgtaaatt tagaaataaa ctcaggtgaa tttgtttctg taattggacc
    10501 ttctggaagt ggaaaaagca cacttttaca tattttaagt ggattagata atcctacctc
    10561 tggacaagtg ttacttgatg ataaagatat atataaacac actgaaaaag aactatctgc
    10621 acttagaaga aaatcctttg gattcgtctt tcaacaattt aatcttttac ctgttctgac
    10681 agcatctgaa aatatttcaa tgcctgtact tctagataaa aaacaacctg ataaagaata
    10741 tttaaatgaa atctcatcat tacttggaat tgcagataga ctcaatcatc ttccccatga
    10801 actttcaggt ggtcaacaac aaagagttgc aattgctaga gcacttattg caaaaccaga
    10861 tataatattt gctgatgagc ctacaggaaa cttggacagt aaaagtggca gtgaagttat
    10921 gaatcttctt ataaaaactt caaaacagtt tggaaagact ttagtggtaa ttactcatga
    10981 tgatagaatt gcaaaacttg ctgatagaaa aatttcaatt attgatggtg ttcttatgga
    11041 ggtgaaatag aaatgaagaa ttacctttca ctttcaataa aagaattaaa aagccagaaa
    11101 cttatgacta cattcatcat tatagcaata gtactatcaa ctattatgac aactgtggtt
    11161 ggacagtcaa ttggtatcct acaaaattta agaattgaac aagcccgttc ttttaacggt
    11221 gatagacatg tatcctttca ccaacttact aaaaatcaag ttgatgacat aaagaaagat
    11281 gatagagttt atcaagctgg tacttcaata actcttggca gtagtaaaat aaaggattct
    11341 ggtatctctg tacttgtaaa agagtatgat aaaacaggac tttccaacta ccccaaatta
    11401 atgaaattaa aatctggaaa tttgcctaaa gataaaaacg aaatagcctt agatgaaaac
    11461 acactaaagc ttatgggtat aaaaccaaga ctaggagtga ctatacctat gaatttagat
    11521 atttcacttt taaatgatac cataccacca tataattaca cagctaactt taaattgagt
    11581 ggcattttag aggatgatta tacaggatat gtaagtggta tagtaaatgg tattgtaggt
    11641 aaaggtacat cagaaaatct attacctaag agatatattt tatcctcttt ggattttaaa
    11701 ataaaacagc aagaaaagtt tcaagaaata gtaaatcaat tagctaaaaa aataaatctt
    11761 tctcacaatt caattcaata taattggatt tacttaaatg ctcttaagat acagtttgaa
    11821 aaagatgaaa atagttctaa tagtgatggt atatctatga ttatattggt atctttattt
    11881 gttgccttat tggttttact ggcctctgga cttgtaatct ataatatttt aaaaatctct
    11941 gttacaaaga aaattaaaga gtatggatgt ttaagagcaa taggagctga accaaaccag
    12001 atatataaga tagttatttt acaaatctta attttatgta ctgttgccat acctattgga
    12061 gctgtaattg gaattatatc ttctaaaggc ataactggaa tggtcactaa tatcttaaat
    12121 ccagatatac ttctagcaaa tgataataaa gaaattactg agcttataca taaaaataca
    12181 acagcatata tgttcccatt agtattaagc actaatgtat ctttgatttt ttcatttatt
    12241 tcagcattac catcagcaat atatgcttca catgtctctc ccaaaattgc tatggttggt
    12301 agtactacca aaatcaaaag aaaaattaaa agagaaaaaa caataaaaaa ctttgaaaga
    12361 catctagcat ggctaaactt aaaaagaaat aaaggaagaa ctataataac tatactatca
    12421 ttatttatga gtataacagt attcgttgca ctgagtgagt tctctaatgt attagatgta
    12481 tctaggtcag taagtaattt aaaagaagga gatttttcct taacaaatga aatatctggt
    12541 tttgataaat caacactaga taaaataact aaaatgaaaa acgtcaatcg cacttccttt
    12601 ataaagtact ctgaatacaa acagggggaa atagatacag atataaattt tgaaaatagt
    12661 ggtgagatgt taaaaatcat tggaattgat gagcaaactt taaaagactt aatgccttct
    12721 attacagatt ctatactaga agactttaaa aatggttcta tctgttttat aaaaaatccg
    12781 attgctattt ctacaccagg tgtaaaaact agacatacaa atttaaaacc taaagatagt
    12841 atcacaatca acaaaaaaca actagatata tatagtacag tagataaaat gttcttttta
    12901 caaggtaatg gctggataaa tggtgttgat gttattgtat atgattctgt ttataacacc
    12961 ttgacaaata aaaacaagtt caaccagata aatatttatg ctaaagataa atcaaaatta
    13021 gaacaaataa gattgtctat tgaacagatt tgtgaaaata atcctggctc tcattggata
    13081 tcatatattg aatcagataa acaacttaaa gagagtttta aacaaataga atttctagca
    13141 tgggcagtta tattatttgt gggattgatt ggtactttaa acatcattaa cacaactcat
    13201 acaaatataa atactaggac taatgagatt ggagttaaaa gagctattgg tatgagtaat
    13261 agtagcttat ataaaatgtt tttatgggaa ggtgtttatt atggaatttt tgctgctata
    13321 tttggttcaa ttgctgggta tgccagtgct attattataa acatggcaac tatagaaaag
    13381 cttgatttca ctaatatacc aattacttca attttacaag ctactattat ttctgtactt
    13441 gcatgtatta tagctactct tattccgtta agaaaagtta aaaagatgaa tataattgac
    13501 tgtatagata tcaatttata actattttac agataataat ataaattaaa ttatcaatga
    13561 ctgtctctat tttctttcaa atctaactaa taaatctttt taactataat ataagcttta
    13621 aaaaaggcct atctcaaaat agagataagc cttttttcat tgtcgaaata atgaaaatat
    13681 atttaacttc acaaaaaaag agtgcctcac gaattaaaaa tttcgctttg agacactccc
    13741 ctttaattta aaatatatag ttaaatgcta tgccaaaatt tcacacatgc tccacctgat
    13801 tttgaatttt ctattttaac agaacctcca agctgttcca ctaattgttt gacaatgtaa
    13861 agcccaagac cagaatgtcc accttttgtc tgcctagctt catctcctcg ataaaattta
    13921 tcaagagcct tttttaaatc tttagagcta aaccctctac cagaatcaca aatctcataa
    13981 gaaatacaat ttttttcatc ctttacagta atactaatat taccacctga tggagtgtat
    14041 tgcagactgt tggatatgat attatcaaaa attcgtgtca acctatccac gtcgatttga
    14101 attggggatt gtatattccc ctgcattttc aatataagtt caacttcctt ttgatgagcc
    14161 tgtaattcat agtcttgcac cttttgcctc aaaaattcag gtaaatttat aggaacaaga
    14221 tttaattgta tatttgactt ttctaagtca gacgtatatt gcatcttttg gacaagagca
    14281 gcactttttt ctgtattttc tcttatcact gtaagatacc tatgaagttt ttcattgtca
    14341 tctgtattat taccaataag tgcatctgta tatcccaaaa taatagaaag aggagatttt
    14401 aaatcatgtg ctagagcttc caccatttca acacgttcct gctccatctt ccactgtgca
    14461 gataaagagc cttttaattc gtctttcatt tcagaaaatg cactacaaag tttaccgagt
    14521 tcattatctg aataatagtc gatttcaaaa tcaaggtcct tttcttttat cttcttggac
    14581 gcatcaatca atagatgtaa tggttgattg atattcttta caaagcgctt agaaagccat
    14641 ctagtaaaac ccacaatata tagaaatgga gaaaacagag ccattataat aactgcaaat
    14701 acaaatctcc ctctaatatt tgcaaaggtg attttcactt ggtagaatag aataacagct
    14761 ccctcaatct ttccattatc ccctttgatt ggaacagtat ggatataacc acctttacga
    14821 acagttttat tgataaaatt attaaataat tcttcttttg ttttaaatag ttttttgggg
    14881 ttagtaccat ataaaatatt gccattatta tccacaactt gatatagcat atcatcccca
    14941 cgaatcgttc tttttaaacc ttcttcattc gattgtgata acaaagttat gtttttttca
    15001 ttgatgtatt tttcaattct aggaacttgt cgctcataat agtttggtgg atagatatcc
    15061 ttatttaagg aatgaatata caaatagaga gcaaatacat aagtaatcac tgtggcaact
    15121 atactggcta ttataataaa aataaaagta tgtctaaatt gggtaataag ggattgattt
    15181 ttcatttaat aacctctctc tctttacatt ttattccact tatacccaat accccaaact
    15241 gtggaaatat attctttttc tggtgtcaca gcagaaaact tagctcgtat tttttttact
    15301 cgttcaacaa cagttgagga atcaccttca gaatcacacc cccatacttt ttcatagatt
    15361 tgttctcttg agaaaacttg tcctgcatgg agtgctagta attcaacaat atcatactct
    15421 atttttgtta aatcaatatt cttaccatca attttaattg cacgttcctt catatataag
    15481 cataacttcc caaaatacaa agtagatctc ttgttctctt cattgatata ctgtgaacgc
    15541 ttttccctac gcagattagc ttcaatcctt gccataagtt cacgcagtcc aaatggcttt
    15601 gttacataat catcccctcc aaaggtcaac cctttgattt tgtcggtttc tgattgcttt
    15661 gcacttaaaa atataattgg gcataaaaca tcatctctaa ttgcctgaca tacctcaaat
    15721 ccattcattt taggcatcat aatatccagt ataataaggt caggctcctg ttttgataac
    15781 tctatacctg cctgaccatc ataagctatc aacacttcat gtccttttgc cttcaattca
    15841 tcctcaagta gcattactag gtccatttca tcatcaataa tcaatatttt actcaatata
    15901 acaccacctt tctcttatgg gaaggtcagt attaaaacaa tattcgtcta ctaataactt
    15961 ttccttccct cccacatttt aaaccaaata acgcctccta tcaagaatat aatcaaacct
    16021 attgctacta ctataagctc tatactagct gtttgcatca ctttagagta aaatgcctca
    16081 gttgaaatag ttccagcttc tttaataaaa taaattcctg gaagcatacc tagttttact
    16141 ggccatgtcc atggaacaaa tgcccatact ttttcaccaa cgctagttaa tccaagtatt
    16201 gctgccatca aaattccacc gatactaata ccaatagatg cacccatacc ccatgcaaaa
    16261 ctcacccaaa gatgaatagc taatagtgga agtgtcccaa tcaccacaag tcctgctgct
    16321 gacaggaata accatattgt tgctccagat ggcactgcaa tactcattcc tatgcaaagt
    16381 atcaacgtag caataaatgt gcatatagtt aggcaaaaca gaagaaaaag aaattttccc
    16441 agatataatc taacacgaga aatacctaca cataaaaaac cattaaaatt tccagcaagc
    16501 tcttcctcct gcactataaa tcctgctaaa atacctactc caataggaat gatgaataca
    16561 gtccatactg taaaaaatcc ttcaaaagca aattcttgtg tacttccaga tttagttgtg
    16621 agataaaaaa ccacacataa tgaaaaaata ataggcatac aaagagtaag ccactggaca
    16681 gctgtcctct ttgctcgaag ccattctgat gataatagct ttatcatttg tcatccctcc
    16741 tattgaacca agctgcagta agtgctgtta atactataaa cacaaccaaa gaaacaacaa
    16801 tcccaatagg aatcactgaa gtattcaaaa gtgggtctcc agcttctaat atagtaccgt
    16861 taggatgtat gttgatgatt gggcatacca ttctaactgc ccaactccaa ggtactgcaa
    16921 tccaaaatgg ttttgtcgca gcaaggacac caaatatcat acctaaagct ccaactccca
    16981 tacttaggaa catacctttc catgtagcta gccatagctg aagtggaagt atagcaagag
    17041 aaacaaccca gcatacaata cttgccccta taatctgtcc aaaaggaact ggaccagctt
    17101 tcaatattaa accagttata attgttacga ctattaaaac tagcgttgaa ataaaactgt
    17161 agactgccat agctataact ttattaatcc aaagtgtcat aggagacaca ttgtgtgtac
    17221 gtaaagcacg ataatttcca gctttttttt cctggaaagc taccaaagta gcaaacaatg
    17281 caaatccaat aggcagaaat aaaaatgacc accagttaaa aattaatgct aaaagatttt
    17341 gccacgccca agaagttgtt tgagataatg gattttgcat gagtgttgat tgcattataa
    17401 atgcatatgc tgcaaaagac actgggaaaa aaacaataag tttacccata aatgttcttt
    17461 tatactttaa aagttctgat tgtaaagcat tcatctgttt tgtcctcctt taagaataac
    17521 atctgtaaaa aattcttcta gattttcatt agggtcaggc attccttgaa ataatagtcg
    17581 tcctccattt ataattccta tattatctac aacttgagca acctctgata gaatatggct
    17641 agaaagaatt acagttatac ctttttttgg aaaagaagca atcaattcac gcaattcttg
    17701 aatgccaaag gggtcaagac cattggttgg ttcatctagt attaacaact ctgggtcact
    17761 caaaagtgca atagctattc ctaacctttg tttcataccc atagaaaatt gtgaagctct
    17821 ctttttacct gtatttttca aatttactat ttccaacact tcataaattt tatctttagg
    17881 tattcccaat atttttgtat gtactaacaa attttcctct gctgtaaggt ttccatacaa
    17941 tgcaggggat tctattaaag aacctatttt tgcaagagat tctcgtttcc aaggctttct
    18001 atcaaaaata atacgaccat ttgttggacg aagaagacca acaagcattt taagtgttgt
    18061 ggactttcct gctccatttg gtcctaaaag tccatatata ctatttcgtt caatttgtag
    18121 agaaacattg ttaactgcca tttgttctcc atatcttttt gataagtttt ttgtttcaag
    18181 aattaaatct ttcatattaa tttctccctt cttgtaatac cttaaatctg ttactaagag
    18241 aatactaaca tgaaaaaata acaattctat aacacctact aattttaata aaatttaaaa
    18301 taaaaaatag agttaccaat tttattttgg caactctatt taagtttatg ttttacagtt
    18361 atattagatt taaacttagt ttcattatca cactaacttc aaatttactc tataatcttt
    18421 aaatcaaaca ctaatttatt aatcaaacac taatctatta atttatattt ctttagacat
    18481 ctaatatagt ttgctactaa ataataatta tttatatcat atttatctaa tcctttaaaa
    18541 gaagttattt catcattaag ctcatcacta ccttctacaa aaacacaacc tgtttcaaat
    18601 tccataccta caataatctt acagccacta taaatttcct cttgagtatc gtacaatttt
    18661 tttgcatttc cttcatttgg aattgtaaat acaataggtt ttaattcttt atcactggaa
    18721 atcttttgtg aaaaaggctt gtttaactta atattattga taactacatg tttaaattcc
    18781 tctgaagttt cttctactat tacatatttt cgctttaaaa actctattat ttctaaaaca
    18841 gacttagaat ttacttttaa aatactttta tatttttcat ataaagattt ccactgttca
    18901 attttttcct gtggtatttc ttggatatgt gtacttattt tttcttgaga tatttttttc
    18961 aattaaactc tcctttcaat acttaaatat ccttatgaat ctcacatgac taaagttaca
    19021 aggtttctac ttaatagaat tttgcatact taaatatgtc ttattaattc tctacaggat
    19081 atctctgtaa ttcctacgat tcttacataa gttacttatt ttaaattgtt attagttaga
    19141 ttctaaaaat tatttatttt atcactaata taatactctt aaaaacttta caaatcaatt
    19201 taaatttgct accttttatt tataacaact tatagtacat ttctcctatg cttttcaata
    19261 tattactttt aaaaggaggt tttcattatg attttaaata gagctgagct tttccgagga
    19321 tattctttaa atagatttaa cgagatatgc aatatattat cagtaaataa aattaaatat
    19381 aagtataaag tgaaaaataa tgcttactct aaaaaacctt tttttaatga aataacactt
    19441 ggcaatttag gtcaaaagga agatttctct tatgagtact taatttatgt tcataaagac
    19501 gattatgaac aggcagagtc tctaataaat tctaagttgc gatagattat ttataaaaca
    19561 tgacattctg ttgtcatgtt ttatatgtta ttatactttc aaatatgcta ctatattttc
    19621 aaaattgtgt gctttaaaac tagcttttta tagtaattta aagcttaaaa ctaaggagac
    19681 tcgttatatg aaaataaata taaaatcaaa gttaagtgaa tttattaaac agaacaatct
    19741 ttttgatgac agcagaataa tatcctacct tcctaatata acaatagaaa cagacaaaat
    19801 aaaaactata atgattaatg aggttgtccc aactaacacc aatgacgatt tttatagtgt
    19861 agataaagat gctgattatt taaaaactac tattccttta tttgacagtg caggaataaa
    19921 agtatctaac ataaatgata tcctagatat gggtatctat ataactaatg cagtaaaact
    19981 tcctaaatca gaatatacaa taactcgtga tacaattaaa ttacatatgc ctattttaga
    20041 agaagaaatt aaattattta aaaatctaga ggtagtaatg ttaatgggag atgttgcaaa
    20101 aaaatctttt aatatgatta ctaaaaagca tataaaaaag aatgtcatcc cttccatttc
    20161 aacatataaa cttcgtaata atgaacttta ttatgataat ttaagagtat ttccatcata
    20221 tattatgact ggtggaaaca tactgattga aaaatcaaaa tttgaaatgg catcagaaga
    20281 tataaaaaaa atgttcgaga ttataaacgg ttgatattac aaaataataa aaactaatat
    20341 aaaataaaaa gtgcctcaaa aacttctatt tatgaggcac ttttatttta tattagtgta
    20401 actatatact attaacataa tttattattt atattgttct ttttaatctc tattccattt
    20461 agtctaggta acattactct ttacaagatt actttatatt tcttttatat ctctaacgtt
    20521 tttatttaat aaaacaatta ttaatacaag tcaaatatat ctatttatct aaaaatatat
    20581 cttttacagt aatatctgga tggtgatact tcttcctatt cgttaaaaaa gattttttta
    20641 cattaatagc ctcttttgga caccagtgaa aacaagctag gcatctttca cactcattac
    20701 cccactttgg ctttttatca actatactta tattctctac aggacaaacc cttttacatg
    20761 tcccacatcc attacattta ttttctgcaa aaaatttagt agttgcaatt tgcatattct
    20821 tacctactaa acgaagtaca acactatcta tatgtgactt taaattgttt tttccttcta
    20881 tatttccaat ctccaagtta ttgatacatt ttgcaatctc agctacccta ttagtctgat
    20941 ttttcaatct ttcttcttca acatctggag gtgttatcaa agcatttcca ggcatattga
    21001 ttaaaaaacc agcagataat tttttccccc tttttttaag taacttatct atagtaaata
    21061 aactatgtcc agctacccca ttacaagtgg caatggcata tatatgagta ttaatattaa
    21121 aatttaattt ctctacaaaa gactttacta tatcaggaac atcagcaaaa taaacaggaa
    21181 aaacaattac caatatatct tcatttatgt ctatattatt ttcttttcta tgtatagcta
    21241 agggtataac ttctttactt ccatttaacg tttcttttaa aatatttgaa actactagcg
    21301 aatttcctgt tccagaaaaa taataaatct tactattcat tcttatccct ctttctatat
    21361 ttctacatca tcaatagtta aaaagtctct taaaatagga tttatattta ctttccaatt
    21421 ctatccaaaa tatcagtaaa caaaggcaat tcttcatcag agatagattt aaatgtctct
    21481 gttgcatata tacttgcaat ttcatttaat ggttcttcta attctctacc taaatctgtt
    21541 aggaatattt gaaacttacg tctatcttta acatctactt tacggacaat taatccctta
    21601 tttttcataa catctaagat tcttgtcgta ttagatttat ccttacatga tttttcacat
    21661 aaatctatct gagaaattcc atcttgctcc catagaaacc ttaataacgc ataatggtca
    21721 gttgttatat catatccatt ttgtttaaac atctttttta aatcatcttt tacttttaac
    21781 gaagtactta ctaacttagt tgtagcttca tatagtttct caaaatcttt cattttacct
    21841 ccatttaaat aatcccttgc tcctcccatc tatcaattat tcccttatat tgctcaatat
    21901 ctttaaaagc cattggcttt aaaacagcat ttatagcaat aaaattatag atttgagaaa
    21961 gaacaggtgg atttttcttt tcctctatgc aataaataaa ttttttagat accttttgta
    22021 tctttgattc tactttttca gttataggat tttgattaaa tccagttttt atagataaac
    22081 taccacaaac ataacctccc gaatctaaga ccatggattt tataaaagaa attgcttttg
    22141 aaccacgtgc attttcatat gtagtaactg ctatacatgg cttacgatac aataatcttt
    22201 caagcatcat atgtgctcta tctgtaaaca ccttgaaaag tccagttaca ctacttccat
    22261 aagttgggct accaaatata acaccatcac tcttagtaat tatatcatga atatactcta
    22321 ctttatcatt ttgattgata cacttgccca ttttataaca atttaaacaa ccaatacaat
    22381 atgatggatt tacctcactt aaatcaaaat aattaatttt tacatctggt attaatcttt
    22441 ctatatcttt atacaagtaa gttaaaactt ttgatgtagc tccatttttt ctagggcttc
    22501 catttataac tgtaattatc atttctatat ctccttttta gttgttatat caacaataag
    22561 tatatcaact atatttttag ctgtcaatat aaaccattta tttaaagcca tacttacaca
    22621 gcttaaataa aagttttatt taactacatg tgcttattta atcaactata aatgaattat
    22681 ttttaataat tgacaaacta aattaatgat aatatacttt atgtattaca aatgtaagta
    22741 gaaggaggta tagtatgaaa aagttaccac atatttcaga agcagaatat caagttatga
    22801 aaattatttg gaaatatgct cctatcagca ctactgaagt aattgaaaag ttggtagaaa
    22861 caagtacatg gagtcctaaa acaattcaaa caatgttatt gagattagtt aaaaaaggag
    22921 ctttaacata tgaaaaaaat agcagagtat tcgtttatac tccacttgtt aaagaagaag
    22981 aatacgtagc tagagaaagt agttcttttt taaatcgttt ttataatggt actcttaatt
    23041 ctatggtact aaacttttta gaaaatgata aactatctga agatgatatt gaggaactga
    23101 gagaaatctt aaataaaagg acgactaagg gggataaata aatatgtatg ccccattctt
    23161 tatacgattt ttactcagta cattaacctt atcaatatta attgttgtaa ttttacttgc
    23221 caaaaaagta tttaaaaaac atatttctgc aaagtaccaa tacaatattg gatttatatt
    23281 gcttataatg ttaataattc catttgtccc tcttaaatat attaatcttg gaaatgtatt
    23341 taactattta tatactttta atcaacttgg aagcagtact aataggactt ctatgataaa
    23401 aaatagtcca aattctgtaa ttaataactc aaatgcatta aacgattttt cagtatccct
    23461 aaaccaagtt aattttgata gtttaaatat tttattacta atatcatgga taattggaat
    23521 tttaatcatg atagctatta ctattcattg caacattaaa ataaaaaata tgaaaaaatc
    23581 aatacaaatt ttaagagatg atgagatagt atttatattt gaaaagtgta aaaaagatat
    23641 tggtataaat aaggatttta tactatgcaa atcacctatt ataaatacac ctattacatt
    23701 tggatacttt aaaccacaca ttatattacc agataagtct atatctaaat tatctttaaa
    23761 agatataaaa tatatcttac ttcatgaatt acaacacttt aaaaataaag atatactgat
    23821 aaattatata atgtgcttcc ttcaaatatt atattggttt aatcctcttg tatggtatgc
    23881 atttaaagaa atgcgtatag accgtgaaat tgcatgtgat atttcagttt taaaaaagct
    23941 tgataaagat agtcatatag aatatggaca aactctaata aactttgtgg ataatatttc
    24001 aagaccatca aatcttacac ttacatcaga tattggtggg tcaaaaaaac aaataaaaaa
    24061 gcgactattg aagataatgg attttcataa tgaatcaaat acgttaaaat tcaaaagcat
    24121 tttaattttt acattgatag ctttaattgt atttggaaat attcccaaac tatctattat
    24181 ggcagatatc aacgaaaaat atagacctac aaatgaacaa actgtgtacg aggacttaag
    24241 tcagtatttt aaaggttttg atggaagttt tgttatgtat gatttaaaat ccaagcaata
    24301 tcatatttac aataaagaaa aaagtaaaac aagaatttct ccaaattcta cttataagat
    24361 ttatagtgcc ttattgggat tggaaaatgg gataataaca agaaataatt cacatatccc
    24421 ttgggatgga aagcatcatt ctaatagttc ttggaatgaa gaccaagact tattttctgc
    24481 tatggaaaac tccgtaaact ggtactttca gactctagat aaaaaaacag ggctaaaaaa
    24541 catacaaact tatcttaggc aaattggata tggcaattat gatgtttcag gtggtatctc
    24601 aaacttttgg ctagaatctt cattaaaaat atcacctatt gaacaggtag aattacttga
    24661 aagtttttat acaaataagt ttaaatttga agataaaaat atagaaacag taaaagattc
    24721 attgttaata tcaaaaaagg atggggtatc actttttggt aaaacaggca ctgccaatat
    24781 aaatggaaaa gatgtaaatg gttggtttat aggttatgtg gaaaaaaatg aaaatactta
    24841 tttctttgct acaaatattc aaagcaaaga ttattctaat ggtagttcag catctaagat
    24901 aacattatcc atattaaatg acaaaaaaat ctataatcct taatatattt actgtacatt
    24961 ctaaaaaatt cacataattt tcatattaat tatcaattaa attcatatac aaggcttatt
    25021 atataaacat agaagatagt aaaactagct taaaaacata atataatacc ctatacaaat
    25081 agtggcaact ctccctcaca tcctcaattg ttgccactat ttaatttttt gtatgaagtt
    25141 aaatatattt tcatcatttc aacaatttgg gtgcaataca atctctattt tgaaacaacc
    25201 tctttaattg ttttagagtt tatgaattat ttctcatact ttaaaaagat tgaattactc
    25261 ttgataatct tcttttgtag tcattacata tcacctacta ctcaacacat attctttgac
    25321 ctatttttac atccttgttt atatccaaaa tatttacctt cttaccatct ggaaacaata
    25381 ccattgtcaa agtagacttt cctaattcgt gtattctctt taaatcaaca ttaacaagaa
    25441 catctccctg tttaaccata tctccttgtt taacagcaac atcaaagcca tctccattta
    25501 atgcaactgt atcaattcca acatgaatta atacttctcc attagttcct ttaatcccaa
    25561 atgcatgtcc acttgaaacc actgtttcta atttacctga aataggagaa acaatttttc
    25621 catctataat atcaatagca aaaccatcac ctaacatttt tttagaaaat gtttcatctg
    25681 gtacattttc taatgcaaca attttcccct tagctggaga cacaaagttt tcattatttt
    25741 cattaaacac taattcttcg tcttcatctt catttatagc ttctttaggc actccaaata
    25801 aacatgttac aataaagcag aatgcaacca caaatattga tactaataag tatgtgatta
    25861 aattatatcc atcatagata tacattaaat aagaaggtaa aactgctaat ccatatgaat
    25921 tagattgaat atgtaaaatt gataatattg ctgctcctaa acctgaagat gcacacataa
    25981 taatcaaagg ctttaaatta tatcgaagct gaatcccaaa taaaacaggc tcacttatac
    26041 caaaaagttg agatagcatt gctccaaccg cagtttgttt aacttgatta tttttagatt
    26101 ttttcataaa tgctagacat gttccaatat tcgcaaatcc atacattgca caaagagtaa
    26161 taagggcatt aaatcctgta tttgctaata gtgatgtttc aatcattaca taagtatgat
    26221 gtaagccagt aataacagct aatggatatg taaatccaat aataaatcct ccaattccta
    26281 aaggcaaatc aatcaataag ctgatgatat caaccatttt taattctatt gtatgcatta
    26341 tagggcctac acctaacaaa ataactaaca ttgttattag cataacaaaa aatggtgtga
    26401 aaataaggtc taatacatta ggcatatgtt tacggaacca tttttcaaga ttagctccaa
    26461 taaatgctgt aacaatagct gttaaaacac ttccttgaca acctacaata ggaataaatc
    26521 caaatgccat aattgcttca atttcatttg atgggtcagc aacagaatat gcattaggaa
    26581 gtattgggga aactaacata agtccaataa caagtccaat aacaggtgtt cctccaaata
    26641 ctttaaatgc tgaccataca ataattgctg gtaaaaaagc aaatgctgtt tctgtaagaa
    26701 ctgaaacaag tgtctgaata tacaatggaa tatttgcaga agacattcct aataatccaa
    26761 gtacattatc attaaacaaa caacccttta atcccaagaa taatcctgtt gctgcaataa
    26821 ctggaataat aggaacaaat atatctgcta gagttctcat aactttctta aatccagttt
    26881 cattattttt aactaattca tcttgttctt tttttgataa agtttttaat cccatttttt
    26941 caacttctgc ataaacttta ttaactattc ctgttcctaa aataatttga tactgtccag
    27001 aagtaaagaa aaccccttta acctcatcaa ctttttcaac tttcttatca ttaataattt
    27061 ctctatcttt aaccatcact cttaatcttg tggcacaatg tgtaatagaa ataatattat
    27121 cttcaccaat aattttaata agctcatttg ctattttatt atatttattc atttttctac
    27181 ctcgctttca ataatactgc atgaaatggt ttcaaacaca ctgttttatt taatagattt
    27241 atatcatcta aattttggaa tacaatatca tatttttctt ctactcttat ttctacaggt
    27301 ttatcagaaa aattataata acaatcaata actaccaaat ctaattgcct tctatacgca
    27361 ataacatctt cactttctat atcaataggt tctattgaac cataaatcaa acaatcagaa
    27421 tactcactat cttgtctcaa ctttatcatc tttttataaa attctaaaat actctctgaa
    27481 ttgttaatct gactctgtgc attaatatct ttataatttt caataacctt taaccatggt
    27541 gttgtatctg aaaaacctgc attttcttta tctgcccatg gaaatggcgt tcttgcatta
    27601 tctcggcttc ttaaattcac aaattctaac gcttcttctt ctgagtaacc ttcatctatt
    27661 gaacgatagt attggtcaat gctagataaa tcattaaaat catttatggt atctctttta
    27721 aaatttgtca ttcctaattc ctgcccctga taaataaatg gggttcctct taaaaagaaa
    27781 tacattgctc ccaacatttt tactgcatca tcattccttt cgtattgcaa aagatacttt
    27841 gttgtagctc ttggttggtc atgattttca ataaaattag ctgcccatcc atgttcctga
    27901 acatacattt gactatctaa aatgcatttt cttaaatctg gaattgtcca tgaaattctt
    27961 ttaaaccact cactaccaga agcaacatct aagtcagcat gtttaaagtc aaagaccatt
    28021 gaaaagaaac catcttctcc aataaaatca cctagctggt catagctaat acctgctgct
    28081 tcagcaacag tcatacaatc atatttttta aacgtatttt cttttaattc ataaagcatt
    28141 ttttctattc caggctgatt tctaactgtt tttgtacatt taacaagacc atcaacacca
    28201 tcactttcta aactttgaaa acttaaattc tttttgataa atgtaatagc atcaactcta
    28261 aatcctgcca ctcccatttg agaccaggta ttaatcattt ggtaaagttc acttcttaat
    28321 attggatttt cccaattaag gtcaggttgt tttttaccaa aagaatgaaa ataatattcg
    28381 tctctattct ctaccttttc ccaaacactt ccaccaaaaa cagacctcca gttatttggt
    28441 aaatcgtttc cttttttaaa aatataataa tcatgataag gactttctgg attttttaat
    28501 gcttcttgaa accaaggatg ttcatcactt gtatgattaa gaactaaatc taatataatt
    28561 ttgattcctc tatctttact ttctttaatt aactctttta aatcttccat acttccaaat
    28621 tcacttgcaa tatccaaata atcactaata tcatacccat tatcgtccat aggtgattta
    28681 aaaattggac ataaccacaa tgatgtaata cctaaatctt ttaaataatc taaacgagaa
    28741 ataattccct gtaaatctcc aattccatca ttattactat cttgaaagct tcttggataa
    28801 atttgataaa cgatttcttt ttgccaccat ttcttttcca taaattactc ctttttatat
    28861 gatatttctt ctttttaatt ctttataaat tccattatcg aatatgtcct cacatataga
    28921 tgtcgctata tctttaagtt gttgatgact atttttcatt gcaattgtaa cgtctgatgc
    28981 ttgaaacatg acaatgtcat tttgaccatc accaaaacat atggtttctt tttgagttac
    29041 tcctaatctt tcttgtaatc tcttaattgc tttgccctta tgaaaatctt tttgaataat
    29101 ttcataatac tgactagaaa tatctctctg tgctaattcc attttatctt gtagtatatc
    29161 tttcacttcg tcaaatacct tttcatttga ccataaacaa attttatgta tatcttgtga
    29221 cttatactct tcaatgttat tttcatatgt tattttttct tggatatgtt gtttatttat
    29281 acaagaatta gttcctttca actgattcat tgtttcaaat atttctttgg ctttttgatt
    29341 catgaaaact ttttcctgac tctcaatcga aaaagcaact tctctctttt ttaataaaca
    29401 aacaacttcc tttatcaatc tttgattaaa agattgatta taaagtaatt caccatgata
    29461 ctgaatgtaa ttacctcctc ctgcaatata tccatcaact cctaatgata aaacatcatc
    29521 ttgtattgta cccatacttc ttccagtaca aataacaaca ctgcaatgat tcttttgaca
    29581 taaacgtata gcatgctttg cagattctgg gattccatat acctcatctc taagcgtacc
    29641 atctatatca agtattaata atttgtacaa tttcatcttc ctttcttact atcaaaatca
    29701 taatagcatt caaaaaaaaa gatatcaata ttttgatatc ttttaaaaaa tatgttcatt
    29761 tttagcacaa attttcattt tcggggaata tgttcacttt ctaaatacat cttgtattta
    29821 atataaagca gttcaattaa aataaaataa ggtgttgtta catcgtagtc cagttgttta
    29881 tctacagaaa tagatataga ttgaatatac aggttttcat cacataatct tgctaaagga
    29941 ttacttatca tttttgtaat agaaaccata taaattcctt tcattctcat ttctttagca
    30001 aaatcaataa ttttagatgc atctccattc aatgaaataa agaaaatcat atcatctgtt
    30061 tttataaaat gattaagtgc ctgaatcata tcataaccat aaacatcata tataattttc
    30121 cctgttggta agaaaatcct tttcatctct ttagcaactc tttcctgaga ataaccttct
    30181 ccaaagacga ttatacgatt tgcctttttc attctttcaa acataatcga acaatccttc
    30241 ttctctatat catctatcac cttattataa caatccatta attggtgttt actttgattt
    30301 ttatattcta cttctgtttc atttaagcgt ataataactc ttaattcact atatccaggt
    30361 aattgtagtt tttgagcaaa acgagaaagt gcacttgtag atatatggta tttgtctgca
    30421 aattcatcta tagaaagttt aatacaatct tctttatgat taagaataca ctcacagata
    30481 tacttatcat ttgctgtaaa tgaattataa tatttattga aaagtccttc aattttcatt
    30541 cttttctcct tctatactct atgtaattca tatgaagcaa atctaataga gcataaaatg
    30601 ctcctacgac ttcataagat atagcttgta atgtatcaag tttttgcgtt tcaacataca
    30661 gattatactc acaaagtgtt gcaattgtat tattatcaag cctagttaaa gaaatcgtat
    30721 gtatatgatt gttgataaat tttgcaattt caattcctgc ttttgtttct ccagacaaag
    30781 aaatgataat aaataaatca cttttttgaa atgattctct cataaattca atttcaccta
    30841 tatcaaataa gtcaataaca catttaccta ctgaaagaaa tattctcttt atttcttgag
    30901 ctaaactttt ctgttcatta cctgtcccat aaatataaat tgtttctgca tcatatatta
    30961 ttttacaaat atcttgaaat gatatttttt gtaattcatt cattaatagt cgataattct
    31021 cgcaaacatc atgaaaatta ttatcattat taacaaattc tagttgatta tttttaagta
    31081 aatactttaa atcagagaat gaatctaatc caatttttct acaaagcctt aataaagtag
    31141 ctctagaaac attacattca tttgcaattt tttcacacga aaaattacgg tattctcttt
    31201 gacaaatcaa tatttgttta ataataattt tatcattttc atttaaatta tcataatttt
    31261 ggttaactaa cgattctaat ctcatttttt ccactcccta aatattattt taatatacat
    31321 tagttgcttt ttctagggaa gagttttaaa taataaggat aaaataatca tttgatttta
    31381 tttttattaa aataaaaata acctgctaaa agcaggcacc aaaaagtact tatcctttcg
    31441 aacatgtact ttagatattg ctttcttcag aagcttcatc tcttcatact ctaaaataga
    31501 gttttttcta actcaaaagt gttttccatg gtagaaatta ctttaattca tctgccattt
    31561 actttttatc ttatttaata attactatta cttgaatatt tacttcttta agtaaatttc
    31621 atagttaaca aagctaaaac tattattata gatgttaaaa tacaaaattg taagtattgt
    31681 tataaatatg cttacagcta caaatataca tgatatatta aaaagaagct gtaaattatt
    31741 atcatacata caagcaacta tgcctctatt aatccatgtt aatttttatt atttaataca
    31801 aggagttcca tgagacatac atatccattc atattttaaa ttatccaatt cgcttataga
    31861 tttcatcatc tttttataat cccaattcca tggattagga taaggaacta tatttccatt
    31921 cttatttttg actaaatcac ctgcaaataa tacatcttca aaaatcatgc agacatgccc
    31981 tggagtatga ccaggagtat ggataatttt tatattccca atctgcatat tctctccata
    32041 aggttcaaca tccttaatga gccttctgga aattgccttt ccaataaact tcttaaaccc
    32101 aggtctatcc ttctctccta taataaaagg tatatcctct acatgagccc atacttttgc
    32161 tcctgttttt tctttcaata atttaatatt agatatatgg tcaacatcat gatgagttaa
    32221 taaaatatgc ttaatatctg taagtttaat gccaagtgtt tttaattctt taattatatt
    32281 ttcccccata aaaggaagac ctgtgtctat aagaataatt tctttatcaa acacaataaa
    32341 acaatttgag ccacgagtac tatctaacat taatacattt tcttttatct tcataaataa
    32401 attcccctta tttggtattt tacctatata attaattaat aaagttataa atagttataa
    32461 tacttgatat taattttatt ttttattaaa gtatactata ggtatacaag tattatattt
    32521 acataagtat accatcagta tactttttta tcaatagaaa ggaataattt atatgtcaga
    32581 attatcaaaa agagataaag aaaaaataca aagagaaaat gagattattg ataaggcaga
    32641 aaaattattt tgtcttaatg gatttgataa tacaactatg aatgaattag caaaagaagt
    32701 tgaatatact aaacgcacca tatacaagta cttttcatgt aaagaagatt tattctttgc
    32761 tgttgtctta agaggatata aaagactttg ggataatgtt aaaatagaaa gtgcaaaagg
    32821 caaaacaggg tttgaaaaaa taaaactttc atactttgct tttcataaat tttactctgt
    32881 agaaccttct ctattatgcc ttatgggaat gattggtatt gtaaactctg aaaaatcgga
    32941 tactgatatg ctgtttaaag aaaagttttt ttcttttaat aaatttatgt ttgatgaaat
    33001 acaaggaatg tttgaaattg gaaaaaatga tagaagtatt aggcatgata ttgaaatacc
    33061 tactcttatg tattcatcaa tatttacact tacaggattt tttaatttac tatctgtaac
    33121 aggaaaatca tacctgaaca actttaatat agatgaggaa aaatttattg aaacaactct
    33181 tgctctttta atagattcaa ttaaagcata aacctaagtt tataaattaa agtttatata
    33241 cttaaatgat aattttttct atctctaata taaaaaatat taaacataaa actaataact
    33301 tagttaatgg aataactaaa ttactgaaag gattaaaagt aaatggctaa aatacttctt
    33361 ttagaagatg atgatacaat tacatttgga ataaaaactt cccttgaaaa gaaaaatcac
    33421 actgtatgtt gttataatac gattgctgaa gctaaaaaaa attttgataa aactattgat
    33481 ttaattctgc ttgatttaaa tctacctgat ggtattggat acgagttttg ccaatatgtt
    33541 aaaagtatag aagatactcc agtgatattt ttgacagtcc gtgatgatga aaaagacatt
    33601 atacgtggat tagatatggg tgcagatgac tatgtggtta aaccgttttt gctctcagta
    33661 cttcaatcca gaattaatgc agttcttaga agaactcatt caaatactaa caatactatt
    33721 acatgtgaaa atataagctt gaataaggtt gaagtcaaag catatttaga taatgttgaa
    33781 gtacctgtga cagcaggaga atataaactt cttctaagtc ttatggaaaa taaaaataga
    33841 gctatgactc gtacaaagtt actagaaatg ctctgggatg tagatggtaa tttcgtaaat
    33901 gataacactc ttactgttac aataaaacgt cttagagaaa aattaaaaaa tccaccttgt
    33961 ataaaaacat taagaggcat aggatataga atggaggaca actagtgaaa actccaagaa
    34021 atatcaacag tacagttgtt tttataatat tctgcttaat tgtaactttt ttattgagtg
    34081 taattctaac aactataact tcttttaatg aatacaatgt actgtctaag gctcttggtg
    34141 catcatatga gtacaatcct aagtctacag aaagtatact aaaatcactg aaaaattcta
    34201 gtgataagga ttttaaaaca ggaaatgaaa ttttatcaaa acatggatat tctaaaagta
    34261 cattttggag taaaaacttt ttttattttc tagtgatatc gtttggtata atttcaacaa
    34321 tggctacagt tgtatatata atcaaacata gaacaaataa gcaaaaactg agtagaataa
    34381 acaatcttac tcaatactta gaagaagtga atttaggaaa agagtctgta cttttacgat
    34441 gtgaagatga attttctcac cttgaagatg agatatataa aacagttggg gaacttcgtt
    34501 gttcaaaaga atttgcttta aaagaaagac aaactctttc agataatctt gcagatattg
    34561 ctcatcaact taagactcca attacttcta tgtcacttat ggcacaatta ctttcagaaa
    34621 attgtccaga tgaagaaata gattatatta acagacttac aaatcaaatc tcaagacttg
    34681 agagactagt ttcatcttta cttacattat caaaattgga tgcaagtact ttaatatttg
    34741 aaagcaaact tatagatgta cattctcttc ttacttatgc aattgagcct atagaaggtt
    34801 ctttaagaga aaaacatcag acatttacta tcgaggcttc tccttcggta gaatttgaaa
    34861 tcgatattaa ttggacttta gaagctcttt taaatattct taaaaattgt agtgagcata
    34921 tagaagatgg tggatatata gttgcttatt atagtcaaaa tcctctatat gttgagatta
    34981 tcatagaaga taatggtaag gggtttgctc aggaagaact tccatatatt tttaatagat
    35041 tttatcgtgg taaaaatgct agtaaggaca gtattggtat agggcttgct ctatcaaagt
    35101 ctattataga acgtcaaaat ggtactatac atgcagaaaa ttgccataat ggtggtgcaa
    35161 gattcataat taaattttac acataaaatg ctggattata ggtaaaatac ctataatcca
    35221 gctatatcta tattctatgt tatctattgt tttagtaata tacttctatt tacatttaag
    35281 gttttattaa acaaacaact taactatccc tatacttgta agtagtctta aatcgaaacc
    35341 tttaaataag ttcaaataga ttttgtttat attgtttcat tttttataga ttctactata
    35401 ttttcattct tcaactgctt gtatcctgtc atatatgatg ctattacaat aaaaagtatc
    35461 aatatagtaa aacctattat tgtagatata ggtgcaaatg gtaaatactc tataaaatat
    35521 atttcattta tacgtagaaa agcatacact attcctattt gcacaggaat actaagtagt
    35581 aatggtgtaa ttcctaagaa taaaccttcc aaaataagca tcttttttat gccttctgga
    35641 gataatccaa cagaacgcaa catagcaaac tcttgtcgtc tacttctaat atttcctgac
    35701 actgtagcaa gtacatttga taatccaatc atagccaata aacctgtaat aaagcctact
    35761 attaaattca tggtctttat tccactagca tcttgttttt cactttctaa aacatcctca
    35821 atactataat ctcctgaacc atagtcttta ttataaatgg actctaattt ttctcttact
    35881 ctaggaatgt cctcacgatt atttacttta aatactccta tcagcctatt tgaacgtaac
    35941 ctacttactt catcataatt tttagatatt tcctgcacca tagacattgg aactatttgc
    36001 attaatgtat actttgaact agtatctact attttaggaa gtgtgcttgt aatagctccc
    36061 actttcatgt tgtaagtata atccccttta tcttcgtcat atactttttc atttaattta
    36121 atgttatctc ctattgaaag atttaaataa tctatatata ctttatttct acgtgtacta
    36181 cgattaatat cttcctcctt attatataca attgaggttg gattttttgt atcataaaat
    36241 aactttgggt ctacagaaat ttgcctacaa tattctgtaa aactcttatc atcaagccca
    36301 attatatttg aaattatacg aaactttcca tcacgttcta taggagagta ctttttagat
    36361 gaagctatat ttttaaatcc tccatttttc gcaagttctt ttgaagcatc tttttcagtc
    36421 aaccaagtgg aagcagacat tttttgataa tatagtgaac tttttacacc ttctgtatta
    36481 tctaattttt caatgaaagc agactctaca ggttcactat tttccaaata aactgctata
    36541 tctctttggt ccttataatt atcacttcca taaacagctt ttgctccttc ttgattggca
    36601 attatatgca aaaaacatgt aagtaataaa aaagataatg ttaaagatat tgttgcagta
    36661 cgataagatt ttttacgagc acgtagtgcg tttgatgcaa gttctccttc tattccaaat
    36721 atctttccta atgaatattt atgcgacttt ttgattttaa cactatctcc ttgtcgtatt
    36781 gcttctatag gagatatctt tgatactttt cgtgctggta taagtgcaga aatccagact
    36841 gttactattg taagtaaaac tgctggcaga aaagcaggaa taccataagt aaataccact
    36901 tctggaacat cagtatgata attaactgaa ttaacataaa ctataagcct attacacaat
    36961 aaccatccta aaaataaacc tattggtaat ggtataattg tcagtaaaaa gccttcaaac
    37021 actacagacc ttttaatttg ttttggtgat gcaccaacac tagataatat ccctaactga
    37081 gtaagtctag catttgcaga cagtgaaaac gcgttgtaaa tcaccattac aaacacggct
    37141 actgtaaaaa ttgctattgt aagatacata ataggagttg aaaattttga tatactactc
    37201 atagatgctt tctgttctgg tgaaaagact aacatcttta aaagataatt actattgtat
    37261 ttaagattgt attttccata ttcattagtc ttccatccca atgatttagc aagttttgga
    37321 agttccttat acgtgtctct tatattttta aatcgcaagt acacagttat actatcatta
    37381 ggttttatac ttgttttatc aagatatcca agagctgtat atgcaggtat tactgatgaa
    37441 gtagaaacat ctacctttcc tactattgtc aatgtagcct ctgaaatttt tttaaaactt
    37501 tcccctttcg attttggggc tatgggttca agaggctttc catttaggat tctatctcct
    37561 attgggagag taattttatc cccaattttt acacttggat tattctcaaa atattgtttt
    37621 gataatgcaa tttcattagc attatttggt cgacgaccct ctattatagt atccttttct
    37681 ggcatagaat tccaatactc tgatgtagca tttctataaa ttatataatc tcttttctca
    37741 tcattaattt tgcctacata ccattgtcct tttaccatta catcatcaac tgaagaaaaa
    37801 ttttcaatta atggtaagtc acttccataa gtatagtcaa acaattcacc atgccagtta
    37861 ccttctttct caattgaaag ttttataaaa tctgtccaca tggtatacaa cagtccacac
    37921 aaagaagaca tcatggttgt agtaagtaat atagctaaca ttatagctat actactacgt
    37981 ttattgcgtc gtacaaagtc taaggtgtat tcttgaagaa tattcatttc ttcaccacct
    38041 tttcagagac aatcactcca tcttccatag ttacaatacg atttgcctct agtgctattt
    38101 tctcatcatg agtaataaga agtattgtct gattaaatcg tttatttgat aagtttaaca
    38161 aatctacaat ttcttctgaa ttttttctat cgagattacc agttggctca tctgctaata
    38221 aaatagctgg tctataaatt aaacttctag ctatagatac cctttgttgt tgtccacctg
    38281 aaagttgact tggaagatgg tttaatctat cagataatcc caatatagat acaatctcag
    38341 agaatctatc ttcatccacc tttcttttat caagtaacat gggtaataat atattttttc
    38401 taacatcaag agtaggtata agattataaa attgataaat aagacctact tttctacgtc
    38461 taaaaactgc aagagcttct tcctttaaac ttgctatttc tgtatcttca acataaatct
    38521 ttccagatgt aggtctatct actcccccaa gcagatgtaa caatgtagat ttgccagaac
    38581 cagatgctcc tacaatggat acaaaatcac ctttttgaac tgataaattt acattattaa
    38641 gggctgtcac tctggtttga tttgaaccat atatttttgt gagattttca cattttaata
    38701 tttccatata caatcatctc ctttatggtt aaattttagc aacctaaaat gacattatag
    38761 tgacatgaat acaaatataa attactatta aaaatattgt ttagtttttg cacattattt
    38821 aaaattataa tataaaacaa agactatgca atatatttta tttatacttt taatatatta
    38881 catagtcttt aaaaggtatt aactttaaat ctataggtat tcaatttaat aatcaatagc
    38941 aagtatttta tgtactaaag gagttttgct ctatatagac tcaatttagt aaaattattt
    39001 aataaatatt acagactata ggtaaataat atttacaaat aaaattaagt ttattccctt
    39061 acactataat atttcttaat tatattaatt gcaatttcct ttacctttgg ccctgtaatt
    39121 tctttattgg aatcatctga tttaatagca aaataatagg tgtcaccttc acgctctagc
    39181 ataccaacaa accatgcatc cacacgttta tttttttcat caaatcctgt tccagttttt
    39241 ccatatacat ttatgttttt atcatttaca tctattttca taatatctct taaaatatta
    39301 atatgctctt ttttaaagtt tgtatctccc tcaaatattt ttgccattgt ttgcacttgt
    39361 tcttttggag atatttgtaa tgaagattct aaccaaaatc catttaaatg tccctttcca
    39421 ttttttaaat ctccttccca ttcgctaata tcgcaattcc catactttaa atcatctaaa
    39481 atgttttgga catatttagc atcaacttta tttatcaatt ttttgtagta ccaaacacaa
    39541 gactccttaa atgcctcttc taaactaaga tttttattcc aatttttatt tggatactct
    39601 gtaccatcat atcccataac tgattctttt gaatttatta cccctttttc taaaccaatt
    39661 aaagttgaaa ctattttaaa agtagaacat ggtgaccttc ttgtttcaat taattctttg
    39721 ttatatatat tatattcttt atttttagta ttataaaaaa tagctcctcc actaatatct
    39781 tcaaaacaat cactatagtc aaccatatta attttttctt ttttgttttt atcattgttt
    39841 tcaattttgg atttatttaa ttcagactta tttaattcag attttatata attcttatca
    39901 gttttttgat ttatatcgtt atgttttttc cctaaataat acattaccat aactacacca
    39961 accaataaga tagctatcca aataaaattc ttttttcttt tcataattta tatcatctcc
    40021 catatttaaa tttataacct actattaaaa tagtatacat tatttgtaat atactccaat
    40081 ctaggttcta cttataagaa acaagactaa atgaatagct gtatctctta tattttcttc
    40141 acctagtaca attatgttga ttaaacctat tgcgaagata attaggtaac tattaataac
    40201 atcctgtcct ccttttaatt tgtgatataa taattgattc cacatttaaa acttactatt
    40261 gtaatattta aatattacaa tagtaagttt taaatgtcaa gttactttgg gaaagttatt
    40321 ttaattgtat aattgttatt acatttataa aatatggcat gtttaaaatt attttgctat
    40381 tgggaggatt aagataagca gtattataaa agctataaat tataaaattt aatatataat
    40441 ttatagcttt tacttttaca aatattgttt ttattgattt attaatgaat tttattgaca
    40501 ggttatattt atataagtaa acacttacat aataagtata taatctacat atctataagt
    40561 tgagtcaatt caccagtata tgtcaactta agtttatgca tagctcgtgt acaagcaata
    40621 tataacaaac tgcggtcata atcactataa tatgttttat tatttactga aggtacaata
    40681 acttcatcaa actctaaacc ttttgacatt ttaatagaag ttatggatac tccttttgta
    40741 aagctagaac tttcagaaga tattaagtga acactatatt tttctcttaa cgcatcatat
    40801 acagctttag cgtcattatc tgtctttaat ataataccaa gagtcgcatt atcactcttt
    40861 ttaaattctt caatttctat ttttatctta tttatttcat cttgctcgtt attacatttt
    40921 aataaagctg gttcttcacc atgtcgttca ataggctcta atgaagatat gtcttgaatc
    40981 tttttggcga aattaataat ctcaaaagta gaacggtaac tcttattcaa cataactagt
    41041 tccccatcat tatatagctg tttcatatca tctagtgtat gagtgtgatt aggattaacc
    41101 aattgtccaa aatctccaag aatagtcttt ttacacttaa aaagtaagtt tattacagca
    41161 tattgaatag gtgtgtaatc ctgcatttca tcaataacaa catgtcttat aatcttatct
    41221 tcttgtatac cttcatatgc agcatgaata tatataaatg ggtatacatc tgaccattca
    41281 agagtttttt ttgttgccat cacaaacatg tttgatatat tcatttgttt aaagaaatcc
    41341 ttatatagtg taagagtatt ttttattgtc aacattccat tcaaactttt aagtatactt
    41401 tttacctttg gtaaatcttc ttccataata ttatctgatt caaatttata atgaatgtct
    41461 tcagctacct tctctaatct cttcttaact ggatatctgt tatatgcctc aaatctactc
    41521 tgtatccagt cacttttagc tgtaaaactt ccaaaagtgt agtcttttgg aataaaaatc
    41581 ttatttggca tttgttttat ataatcatca ataagtttta caaaatcaag tgtagattta
    41641 aaacgcactc tttcagacca tttagcatca tttatttcta atgggtcttt ctcactttca
    41701 aaattaataa ctctatctaa ttgtacctct gctatatttt caaaacttaa ttcacataat
    41761 ggctcttctc caagctcagg aaggacattg gaaatatagt ctccaaagac tttattaggt
    41821 gataggataa ttacattatt tgctgatatt ttatctttaa atcgataaag aagaaaagca
    41881 attctgtgta atgctataga tgttttccca gaaccagcta ccccttgaat aatcagtgta
    41941 tcagctttat catttctaat aatttgattt tgttcctttt gtattgttga aatgattgac
    42001 ttcatttttt catctgaagt gtgactaagt tctttttgaa gaatatcatc ttgtatatta
    42061 atggaacttt ctagtgcata ttctaatttt ccatttttaa ttttaaattg tcgttttctt
    42121 gttatttctc catccaccca tcctattgga gcatcatatc cagcttttcc gatttcataa
    42181 tcataaaaca tactggcaat tggagaacgc cagtcaagta ttataagctc atcttcataa
    42241 tcaaatgcaa atctaccaat ataatactta gattcatcat ccatatcttt cagcctaaaa
    42301 tctattctag caaagtatgg agaatctttg attttattaa tcttatctcg aacttttacc
    42361 ataaaagccc catagttatc tatttgtttt agtccttgtt cattttgaaa catttcatgt
    42421 gggtctattt caccacgatt ttttaccata tacattttag cgtccatata atccttatca
    42481 agcttgtcaa caagattttc agctttctta aaagcattgt ctagcttgtt atttatctct
    42541 attagatgtg caatctcatc tggaaaaaaa gttaataact ctgcatcata ttcacacata
    42601 ttatgtttag aattagatgc aataatatcg caatttacta cttgtttatt ttcagacatt
    42661 taaatcctcc tttgttaatt aagtacttac actgaatcat tatacttaat tgtgttttgg
    42721 agtttttaat cattcttgct atttaactta aaggaagaat aaatttttga gtaggatatc
    42781 caaattccac aagcaactct gcttctgcaa agcccaactt ctttatcata tctctatgtc
    42841 ctgtatcagc tttgtcaccc tctcgaaagg tagtaattga aatatctgta tctatcaaga
    42901 aatcttcaat aactttcttc aaaaacactt gtgcaatgtc tgaattacga tactgtggat
    42961 gtattccaaa aaaatcaata cttctagttt ctctattaaa caccatattt gcaatagcga
    43021 cattattaag ttttaatatt aaagctcttt cttctaaaat atactgttct aattgatata
    43081 tatattcttc ctcttgtaaa tttggaaatc catctatgac taatctcact aaattcatcc
    43141 ataatggtat gtcagacata cttgcaagtt taatttcttt ttccaactct agtacattct
    43201 ttgaaaatag gttatctttt ttcaaaacaa atcttagctg caaaggataa aattcttgtt
    43261 ctttcctata tttatttgga gattttttat acatagcttt aaatatagaa gtgaatgcct
    43321 gttgactttc atatccagct atcaatgcaa tttcaataat tggtttcttt gaaaatgcca
    43381 ataattttgc tgcttctgtc aactttctcc gctttatgta atcatgcatt gttaggccaa
    43441 cagaagttgt gaaagttcta tgcaggtgat attttgagta atgaacagca ttagcaacta
    43501 tatctaaatc cagcttttct gataaatgct cctctatata gtcaatagca gttatgacat
    43561 taattacttt gtaagacatt gcagcttccc tccttttatc catgcaacat tatatcaatt
    43621 attacttagt tatatttaat gaatcttgct attttataaa caaatttgtt gtttaagttt
    43681 tcaattcata aatttaatat attacttttt actttctgat taaagactct aatcctttaa
    43741 ttttcaataa tttttcattt agtaaatcta aaaagttatt tattattttc ttcttattag
    43801 attcctttta tattccttaa ttaaatgata aatatttata ttatacgact aaaaacagct
    43861 ttttacaatt atttaatatg attacaaact taccctatct taaagtttaa tggggtcctg
    43921 ttatgaatct ttttatttta atttaagctc ttcatcagtt tattttatat attgatgtat
    43981 ttgatttcat acaaaaaaga gtgcttcatg aactaaaaag ttcattttta aacactctct
    44041 tttatgtaaa taaatttcta ttctattttg aaatattata atttgactta taatctgtta
    44101 ataccaaaaa taataaattg tacgtctaag aatagattct aattagttgc tcagtagtaa
    44161 aatctgtttt taactaatcc tgctttgtat gttgaacata ttctataatt gtaccatctg
    44221 gatgtctcaa tgtcatatta tatcctgtag gaactttttt cttatctctt actacaactg
    44281 ctccattatc caatagataa cttctccatt catcaacaga atcaaccata aaagtagatt
    44341 ttgtttctat aaatggttta agagcatcat cagaaccaga taatagtaaa acatttccta
    44401 tttgcgctaa ttccaaccca acttctttat aggaaaaacg caaagaacat ttttctttta
    44461 aaagattttc ataaaaaggt atggcttttt ctatatcttg aatataaaaa cgtgttaata
    44521 tatcatatat tttcatattt tccccctata tatctatata atattacctc taaattatac
    44581 cactctacaa attatataac aataaattaa gcatagcctt ttgagcatct taagtagacc
    44641 ttaacaatat attggggtta cattagaata cataaattaa atcaccttga tattatttag
    44701 gataaaattt atgtagtagc agtaaatgtt cttctaatta tcaactaaca aacgataatc
    44761 tttaactttc tatataatgg gtaacatcat tttcgtaaga tattcactag ctggacgatt
    44821 gatatcattt aaatactggt aatatatatc gccatgaagt ttatatccat tgctatttgc
    44881 ccatgaaaat aagtcttcta aagtagggtc ttgtaattca taaggtccta aatcaattgc
    44941 agttattatt ttctgtgcag gaacaatcct ttgtagaatc tcatttttcc catctatata
    45001 agtggctaca ggaaacccta cttcaacatc caattttgct aaatccatgt tatgaaaaca
    45061 tacaatcggt gctcctgaag ataaaattcc tctctcctcc agatatttag aaattttttc
    45121 aaaactttgt tttgaaaact ctgaaaactc taccataaag tcaattgttt ttcgtattgc
    45181 caaaaaacag tactcagatt gttcagctat tgttatattg gatattctag tcattttatt
    45241 cctccattac tttattttca tttctaatct ttttatacca aattatacga aaatttactg
    45301 tgtaactaat tatttcatat gaatataaaa cataacctcc ttccatttct tatatcgaaa
    45361 gtatagcaaa aataatctca tcattcttgt cacacagtgc tcttataatc agaacatact
    45421 taaaagactt gcctaatttt taggcaagtc taaaagaaat ctttttctat aaaaaattta
    45481 tacaaacaat taaaagatca aaatatagac taatataaaa ctataaattt actaatctga
    45541 acctcccatg gctaaagtca caagattact gatttataga atttcgcaca ctaaaatatg
    45601 tcttactaat tccccacagg ctacccccct agttcctacg ttcttacata agttatctat
    45661 tatcaaacat caaaatttaa acaagtttca ggttcatctt ctattcttgc tattttaaac
    45721 ttacatgcaa ttaatactaa agatactatt ggatttataa tatttaataa agcgtatgga
    45781 ccatatgtta gtgcagatac acctaatgtt gctgttatat acgccccgca tgtattccat
    45841 ggtactaatg gagaggttaa agttccacta tcttctaaag ctctcgacaa catcttagga
    45901 tgtatacctc tctttttata ttccttatta tacattcttc ctggtataac tatagataga
    45961 tattgttcac ctgctattat attggtaatt gtacaagtta ccatagtaca taatactgtt
    46021 ccaaatacac cctttgctaa tcttagtaaa gaagatgcta ctacttcaag catacctgtt
    46081 ttttctaaaa tacctccaag agataaagcg catattgtta aggatacagt ggacatcata
    46141 ttcataatcc cacctttaga taataaagca tctacctcct gtatttgtgt tgacgaaaca
    46201 tatccatttt gtgctgcatc taatatcgta ttcatagatt ctccttgaaa aactactgca
    46261 aacaatactc ccaatacagc acctactata agccctggta tagcaggaac tttaaataca
    46321 accaatccaa ttactattgc tggagctaat aataatactg gtgataaagt attaaatgta
    46381 gaatctaatg tagacctaat taattctatt tgttttatat ctagtgcttg acctgaatac
    46441 ttcattccaa ttacaccata aattactaaa caaattaata atgatggtat agtcgaatat
    46501 agcatatatt ttatatgtgt aaacaaatct gttcctgcca ttgctggagc taagttagtc
    46561 gtttcagata aaggtgatag tttatctcca aagtaagctc cagatattat agaacctacg
    46621 attaatccaa ttggcatacc taaactttga ccaatgccta aaagagctac tcctactgta
    46681 cccattgtgc tccaagatga acctgtcgca agagatacta ttgcacagac taatgttgat
    46741 gctactaaaa aaatatttgg tgataaaatt tttagtcccc agtaaatcat acatggaact
    46801 actccactta ctatccaagt tcctataagt acgcctatta taattatgat taagattgct
    46861 tgcattgcca tctttatact attaaacata cttaattcta actctttcca tttaaatcca
    46921 agtctatata cagccacaag tcctgctact gctgctgctc ctattagtgg tacatgaggt
    46981 gatgaaccat acactaatac tcctagactt aaaaataata tcaaacaagt aattggtatc
    47041 attgcatcaa acagacttgc ttttctacat tctcgctctt tcataaaata gtttcctcct
    47101 aattatttaa acactacact ataatttttt ttgaaacata tttgctcgta ctagatgaca
    47161 tgataccccc ataagttaat aaaaattcta aattgtcacc taatttataa tcatattttg
    47221 tgttacttac atctagtatc atgtggtctg aacttgcccc taatattttt atatcagtgt
    47281 ctattggaat taaagagtca atatttatat cttgtttacc tatagctatg attgcccttt
    47341 ttaagattcc cttatcttca taaacaggtt tatttctaaa ggcatcaact cctatttctc
    47401 caataggaac tgatggtttt tctttacatt caactacttg gcatattaat ttaaatgcat
    47461 cttgatatgt tccatttata ttttcaccat atgccgtttc tctaccaagt agaatggact
    47521 cgcctattct caaattagta ataccttctg gcatattatt atttatcaac agatgaatag
    47581 aacttgaatt acctccagat actatttcta gatttattcc aaatttttct tccattcttt
    47641 ttgctatact taccagtctg cctatatttt ccctagatgg tataatagct ccataacagg
    47701 ttaagtttgt agctatacca attatttcaa tattttttaa tcttactatc tctcttacat
    47761 tactaaaaaa gtcttcttca taaaagtatc cttctcgtaa atcgcctaag tcaaacataa
    47821 gaactatctt atgaataata tttttacttt cacataattc atttaatttc tttattgttc
    47881 ctaattctga gttaaaacta atatcgcaat aattgataac ttcttctaat tcacttaaca
    47941 ttgggattct aatcaatatc ttaggtagat ttatatcttg taaatttttt aagttttgta
    48001 ttcttgaatc tgctatatgg tctattcctt cactacatat ttcttctact atttcttttt
    48061 gtgcacaaaa tgatttagta acaaaagata tcttaatatt tctttcatga cacatctgcg
    48121 aaattaattt tgcattatgc tttaactttt ctatatctat ttctaaccta ggatacataa
    48181 tctttcctcc tcaaaattca aactttttaa caggagctta agagatgctt cttcatattt
    48241 tttagataaa acacccatag atgccataat atagtcatta tctattaata tctttgtttt
    48301 ttcctttgga aatagtaatt cattattttt attgtatttg agcatattat ctaatatttc
    48361 aattctacta gggagcctag taagtgctcc ccctgtacca actatatatc ttacttctgt
    48421 taaatctttc ccttctgcaa ttttgacttt tccagttgtt ccatagatat ttcttatttt
    48481 tcctgaatgt cttagcatag ctttaacaac agcttctttt gtcaatcttt caacaaataa
    48541 tatttcttct tttgattttg gtattggagg ataattctct attactgcat caatatctat
    48601 agatagttca ctttgtaagt tttctttacc tataacttct actatattct tcatatttac
    48661 atatacacct aaatccccct caacacttct ctttgcagta ggttcaggat taaccaaaat
    48721 tttattaata tagtcacttc catctgtaac agaatgtaca tcagtagttg ctccacctac
    48781 atccagagtt aataaatctc caatattttt atataaaagt ttagaagctt ccattacagc
    48841 tcctggggtt ggagtaatat tttcattaac catttcttta atgtatttca ttcctggtgc
    48901 tgtagttatg tgttcttcaa atacactttg aatgattctt cttgtaggtt ctatattcaa
    48961 taaatctatt ttagggtata cattttcagt tatataaagc ttataattag tatcttcaaa
    49021 tattaacctt atttcctctt gattttcaac attaccagca tatataactg gtatgcctag
    49081 attcatagaa gctatcattt ctgcattata aatagctgta tctctttctc cataatcgac
    49141 tccaccagct attaaaatta tgtttgggtt tatttctttt attttattta aatcagttct
    49201 tttaattcta cctgatgtta tttgacgaat aacagctcca gcccctaaag ctgcttcttt
    49261 agctgctttt actgtcatat catagactaa tccatgaaca gtcattttta atccaccagc
    49321 tgcactagaa gttgcaaaca tatcattata ctttatatca tctacagata atttattagc
    49381 taaatcttta atagcacctg ataagccatt tcttacatcg ccaccttcaa acactgttgt
    49441 tggagcttga ccttgaccca aaaatatagg attgttagta ttaatatcgt ggaaagcgtt
    49501 taccactgta gtagtacttc caatttcagc taccaacaca tctattttta aagaaacctt
    49561 gctattttcc attatttatt actcatttct cttctttttt caactaagaa agtggctaca
    49621 tgtatgcctt tgctgtttct tccaaatcca gcatctatgc cttgttcaac agctatttca
    49681 ggtgttactt gtgttcctcc acacgcaatc attactttat ctcttatacc tttttctaca
    49741 caataatcgt gtaatttttt catgttttta tagtgaatat catcatgact tattattgta
    49801 gatgcaagta ttgcatctgc attcagttca atcgctgcat ctaccaattt ttctacaggt
    49861 acagaagtcc ctagatagtg gcattctatg ccatattttt caatacctcc atgttttata
    49921 tctattattt ctcttaaacc aactgaatgt tcatcttctc caaccgtagc tgaaactatt
    49981 ttcataggtt tttcttctat atctgctctt atttcttcat cagttagtac cttaggttct
    50041 tctggtatta ctaaattgtt taaatctata tcaaacggaa ctcttccttt cacttctatt
    50101 ctagtccctt cagattcatg cataacttct ctacttatga cctcaatttc ttgtaagttc
    50161 atttttttga caaattccaa agctgcaaac tctgctgttc ttttatcagt aggaagcatc
    50221 attgttatca ttacaattcc atctgcacac cattccattt caggcttaat tttagttcca
    50281 tttctgtact catatgattc ttttagtcta ttttctacat tatcaaaatc gtcaagctca
    50341 tcaatatata ttattttgtc tggattttca aatgtacatc catctattaa cattgatgga
    50401 ttatctatag cattttcatc atattgagct acattattgt atccaaagtg agcagttaca
    50461 ggtgctaaat aatcttcttc tctttcataa acagtacctt caccaactcc accttttatt
    50521 tttctagata ttccatcacc atttctttct ggataatttc cagaatcaac aaaaaatcct
    50581 tgttccacag attcaaaata tccaccaact tcagatattt cttccatata taatacagct
    50641 ctttctttaa gttctcttgc tttgtctctt aattcaccga catctttaag ttctatcata
    50701 tccataagcc catccatacc aactaaagct tgctttgctg tatcacatgc ttctatattg
    50761 tatatatgcc aaggtacatt tctaccttca tcaggtgtta tagttgattg tatgtcagct
    50821 ctagttaata ctgatgttaa taagtttaat acatgagtaa ctgttgcttc tctagttgat
    50881 gattccatat atttagtatt catttgagct ctcatcttat aatcactaaa taactctcta
    50941 agtgcaaccg cgtaaggtaa gtctattttt aaacatggtg ctggaggtgc tgttggtgga
    51001 actgttgata agcatatatt acttttatct atacctattt tttctgagaa tatagagttt
    51061 aaagcgtgtt gaaccataag ttcaggcatt accttccatg cttctctagc agtagcatta
    51121 gcattatgtg ctccatcaat ttgagctata ttggcaaaag ccattatttt ctttgcctca
    51181 caagcatcta caaaagacct taccatgttt atattcctat acagaacatt atattgtggg
    51241 tcttgatgtg ctccattgac tccttcctca gcaaacatta ctgctacttc aggaccagca
    51301 acaccactta catatgaatg gtaattaata ggtctaccaa cttcttcttc tataaaatca
    51361 agagccttcc tttgagctct tacttgtttt cttgttattg gaactccacc tataccttgt
    51421 ggagttcctt ctataagccc atcaaaatgt gattgtccag cagttctgat aaccattaga
    51481 tggtcagctc catgatgagc tgccattctc attcttctta tatcatcctc aaatctacca
    51541 gaagctattt cagtagtaat aactggactt ggttgagggt ctatattatt gaaatactta
    51601 gatgatggta gccctatact cttctttaaa ggttttgtac agtctttata tttaaaaggt
    51661 cccatttcaa gattttccaa gtgttctctc caaacccaac ctcttcgttt tggtttatat
    51721 ttatccaaat cttttaatat atagtctata tccaattttt tattttcctt taacatgatt
    51781 ctaccccttc tctaaataaa catgtaactt catcccaata ttcaccctta actaataaca
    51841 aacctgcttc tctaaccgtt atatttttag atttagccaa tttataaact acatgccctg
    51901 ctcctttacc tataagacct ctttccataa ccccttctaa aataggtttt acttcaagag
    51961 aagaaaatcc cattctcaac aagacagacc tttctattga tggtgttgta ttctttttac
    52021 caagttctat cattggttct actatctggg tagctaaact ccaaaacctt tcttttaact
    52081 catcatcact cagttcttga agatgttttc ttctaacttc aaaatcatcc tctctttttt
    52141 tcatgaacat tcctccattc gtattgattt aatcgaattt tctgaatttt tattttatat
    52201 taaattaaaa aacaataatt actataaatt atttaataca tcaacaacaa aatctcttga
    52261 actttttact tcttccatta aaaattctat atctcgatta cttagcttat caatattatc
    52321 aatgtgatta ttagatatac aattttttat aaatgacttt cttattttat tcatatcaat
    52381 atcaacacat ttcaataatt ctggtctttc tggtaatatt aagtttactc ctgcaatttc
    52441 ttctcttgga tttccaaatt ttatatctat tccattttct cttgcaaagg taagttgtgg
    52501 acatatatgt ttacctgcac ccgtatattc tgtttcttgt accactatag ctttatcctc
    52561 atctagctct tgagctaaag agaatgctgc tgctaaagat gtatttcctg ctggacctct
    52621 ttctatacct tctagctgtg ccaataattc agttgtataa aaaacttctc cttgttttac
    52681 taatacatat ctatccatat atctaagtgg tctagctgca gacctaggta catctgacct
    52741 atcaggccat gtacagtatg gtatcccaaa acctgtatgt cctgttgtaa atgatttttt
    52801 attaaattga ttatcactag ccatgtgtaa tccttttaaa tctacacttg ctcctactac
    52861 tttagtatta atagaaccag cttttatcaa tcctcttgca gttccagtaa gattaccacc
    52921 acctgcattt gtacaaacaa ctatatcagg gtctttttta tacttttctc taaattggat
    52981 agctaactca taacctaaag tttctacacc tgcaattcca aatggtgaat ataatgatgc
    53041 gttaaaatag cctgtttctt ctaataaaac taagaacgta taaaataact ctggtccaac
    53101 agttaactga acaacttctg ctccatatgc ctcacatttt cgcgcttttt ctatgatttc
    53161 aggttgacct acaccattag aatcataaca ttcttgaact attatacatt ttaatccctg
    53221 cattgccgct tgtgatgcta cagctgcacc atagttacca ctagtagctg ctattacacc
    53281 tttatatccc atttgtttgg catgatatac tgcagtagct gctcttcttg ctttaaaact
    53341 accagatgca ttcattgctt catcttttac aaatattcta gctcctttac cttctttagc
    53401 acattttcta gctaatttag tcaaattttt aagctctatt aaaggagtat ttccaacagc
    53461 atactgtgat tgaattgctt caatatcttg taatgtatat cctgtttcac tcatcatttt
    53521 ctcataatca aaccctattc cctcttgttc aaaagaagaa taatccaatc cgattgcatt
    53581 tttcattatt tcgttatttc ttcccataac agcttgataa ctcatatctt tcattgatgt
    53641 tgaattattc attatataca cctccaaata atatttcttt agcttggatt cctatttgga
    53701 gtaactctgg tatacattta ccataatcat gagtataata tggatttatt ttaagtaaat
    53761 taccttttac aagtctgcct gtaattgttt taacttctac taagtcgcct atagaagcat
    53821 cactttgtat aaaaccttta acccacattt ctaaggatac tttcttagta tcctctggaa
    53881 cttgtggagc tctttcttct ggagtcaata ctatattgtg tataataacc caatcattaa
    53941 ttttagcatc cattttttca tccccttatt aattgactat tgccttacta attaatttac
    54001 tgttatttta aattaaacat tattttacca attagctaac tgttatctta ctaattagct
    54061 agttgctatc ttactaatta gcctttgatt cttcttccaa ttcaacttct atctgatttc
    54121 ttatatctcc cattattgct ctaggtactg gtatatccaa catagtttta agacctggtc
    54181 tagcatttat tattaatgga atagagttta ctatcattgc ataagtacct actcctccag
    54241 gtatttcagg atttatttgt aaatcaatgt ttggtatacc ttttattgat acatagtctc
    54301 ctgttttaat accttctgct tctggtatta tctgttgtgg atgctccatt tcaattaaaa
    54361 cttctccatt tacatatcca tatccacatt gtctacaacc agcaacattt ccagccaatg
    54421 cctctccgta cttagatttt ctatctactg tagtcattat tggctctttt gtttgttcta
    54481 tcttatctaa tttccaacct aatccatcag ctatcatatt tattgattct acaaaaccta
    54541 catgacctgc tatagtctta tcttctacac cttttataaa ttcttccctt gttactccaa
    54601 caccttgttc caccataaca gactttccaa atggagataa atcatttact ctcttagctt
    54661 ttatactagt aacttcttca caagtacctg aaagtgccaa tactagtaaa tctaatacaa
    54721 atcctggatt tattcctgtt ccaagtatac ttactccatt ttcctttgca acttcatcca
    54781 tttttttagc taaatctgca tcatcagctt gtggatatgc catttgttct gctgttgata
    54841 taacatttat ttttctattt aataagaaaa ttattttatc aaaagccttt ttagtaaatg
    54901 aatcagttgc taacaaaaca acatctacac ttttttctct aaaaacttct tcataatttt
    54961 tattgataat aacttctggt ctttctcctc tttcaattcc taaaacatta tacatacttt
    55021 ttcctgaagt acttctacta caaacagata ctatttctat accttctttc tttagaatca
    55081 tattagccat acctattccc atagctccaa atccccatat tcctactctt acttttctca
    55141 tatatacacc ctcctaaaaa tttatatatt ctcaataatt agttaaagca taaatcgtgc
    55201 caactttaaa attaaagtaa atatttattg gaacgttgaa actataatta ttttattatg
    55261 tattttcata aataaaaaat aatatttctt atgagaaata aaaaaaagag caaaatattt
    55321 tgctctttta taataatttt gctcctctat tttgcttttt tattttactt tgattttgtt
    55381 tttttatctt gcttatttat aatattttga tattatattt ttttatttta tactgtaaat
    55441 tttgcctatt catacctaaa attgatgcag ctttagttat attgtaagat gccttttcta
    55501 atgattcata tatatacttt tcttctatct cactgataat cttactaagt gttttgtcct
    55561 ccaaatcgtt taaagaataa ttgttactct ctttattctc tttaagattg taatagtcgt
    55621 ttagattaat catacttgtt tctatttgta cctcatcttc catcatactc atagtacaat
    55681 aaattatgtt ttctagctct cttatgttac cttgccaatc gtaatttttt aaaattttaa
    55741 tagcttcatc tgaaattcct accacttttt tatttaactt tttattatac tttgaaataa
    55801 attttttgca caatagaggt atgtcctctt ttctatctct aagaggagga atttttatat
    55861 gaattatgtt aagcctataa tataaatctt ttcgcatttt atgctcgttt aaaattacct
    55921 ttggactttc atttgtcgtt gcaataattc ttacatctat aggtatatcc ttagttcctc
    55981 caacccttct tacataacct tcttgtaaaa cccttaatag tttagcctgt aacataatag
    56041 gcatggaatt tatctcatcc aataacaatg tccctttgtt agcttgttca aataagcctg
    56101 gcctatctat tgctcctgta aatcctccct tgctagttcc aaaaagaata ccttcgaata
    56161 gtgattccgg caaagctgca caattttgtg ctataaatgg atagtttttc ctattacttc
    56221 catagtgaat ggactggcta aataactctt tacccgttcc agtttcaccg tatataaata
    56281 ctgaagcatc agacttgcaa gctttcttag atttagctat tgcttcttta atcaaattac
    56341 tttcccctaa aatatcatca aattcataac cctttttttg tctactacta actttattga
    56401 ctaaattttt tttataattt ctatttagta aattttctat cgttgtcata ttctttgata
    56461 tctcaagtgc ccctacaaac tcgcctttaa tatcaattgg tattgtagta ttaattgctg
    56521 ttatctcttt tccattttta tccagatatt tttgaatatt gtctttgata acttcccttt
    56581 tttccaaaac ttctaatagt gtactcctgt ttattttcat tttgttgact atatcaaaaa
    56641 atgaattggt taaaatatcg tgtctgttca ttttttccat tttttgtaca gaattattgt
    56701 agattatagt tttcccgtct ttattaacaa aatgtacccc ttcatcaata ttttccaata
    56761 tttcattcat tattttaaaa aatatctctt ggttcaacta tatcacccca gtacctaaat
    56821 atataaaaac ccaaaaaatt aataaaatgt tgctattgta tttatcttat catatattta
    56881 tgatattttt tatgtacatt atattaatat atttttaagt at
//
LOCUS       DAGSRM010000015        55883 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.015, whole genome shotgun sequence.
ACCESSION   DAGSRM010000015 DAGSRM010000000
VERSION     DAGSRM010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 55883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55883
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.015"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            78..152
                     /locus_tag="MJE33_001018"
     tRNA            78..152
                     /locus_tag="MJE33_001018"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Glu,seq:ttc)
     gene            167..240
                     /locus_tag="MJE33_001019"
     tRNA            167..240
                     /locus_tag="MJE33_001019"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:199..201,aa:Gly,seq:tcc)
     gene            246..321
                     /locus_tag="MJE33_001020"
     tRNA            246..321
                     /locus_tag="MJE33_001020"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:279..281,aa:Val,seq:tac)
     gene            327..403
                     /locus_tag="MJE33_001021"
     tRNA            327..403
                     /locus_tag="MJE33_001021"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:361..363,aa:Asp,seq:gtc)
     gene            414..488
                     /locus_tag="MJE33_001022"
     tRNA            414..488
                     /locus_tag="MJE33_001022"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:446..448,aa:Gly,seq:gcc)
     gene            498..571
                     /locus_tag="MJE33_001023"
     tRNA            498..571
                     /locus_tag="MJE33_001023"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:532..534,aa:Arg,seq:tct)
     gene            1555..2394
                     /locus_tag="MJE33_001024"
     CDS             1555..2394
                     /locus_tag="MJE33_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBY9646983.1"
                     /translation="MLDINSFLNGFFNIILRMSIYDLIDISIVAYIFYKIFMFIKDTR
                     AEQVFKGIIFLLLATQLSNTFKLHTVYWISLKALDYGVIAALIIFQPEFRAGLEHIGR
                     AKFNLFGKNVNTSEETLNRNIEEIVEALYSLSRQKIGALIIMERETRISDIINTGTII
                     DAEISRQLLINIFIPNTPLHDGAVVIRDSKVKAAACFLPLTESKDLSKDLGTRHRAGI
                     GVSEVSDCITLIVSEETGGVSIAKAGKLYRDISRERMMNILRSNLKTNTETRSFFKGG
                     IFK"
     gene            2391..3572
                     /locus_tag="MJE33_001025"
     CDS             2391..3572
                     /locus_tag="MJE33_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9646984.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMSDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVYGTIEDPIQGQKEVY
                     LKANIPQGVTLEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKRNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVSLNDNKDNDVLKNYKLSKEQVTIKGKKDDIDKISYIKTKPIDLADLANGE
                     EKEVSLEIPEGITLEDSSITIKLNDTKQLSAEFLYNAGDIELRNIPTNMDNPNVISDV
                     DIKVVVESTEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            3876..4778
                     /gene="ptb"
                     /locus_tag="MJE33_001026"
     CDS             3876..4778
                     /gene="ptb"
                     /locus_tag="MJE33_001026"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HBY9646985.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDISEPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            4800..5879
                     /gene="buk"
                     /locus_tag="MJE33_001027"
     CDS             4800..5879
                     /gene="buk"
                     /locus_tag="MJE33_001027"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBY9646986.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLV
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            5869..6546
                     /locus_tag="MJE33_001028"
     CDS             5869..6546
                     /locus_tag="MJE33_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBY9646987.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAVSAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            6589..6804
                     /locus_tag="MJE33_001029"
     CDS             6589..6804
                     /locus_tag="MJE33_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBY9646988.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            6833..7912
                     /locus_tag="MJE33_001030"
     CDS             6833..7912
                     /locus_tag="MJE33_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBY9646989.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKRKPNVINSLYLEPEV
                     LEDHCWHLQEKFDAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGKLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            7912..8664
                     /locus_tag="MJE33_001031"
     CDS             7912..8664
                     /locus_tag="MJE33_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBY9646990.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            8665..9222
                     /locus_tag="MJE33_001032"
     CDS             8665..9222
                     /locus_tag="MJE33_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBY9646991.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            9392..10738
                     /locus_tag="MJE33_001033"
     CDS             9392..10738
                     /locus_tag="MJE33_001033"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBY9646992.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCLPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSSPDGNNINYKCGS
                     THPEQLQRAVLEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSEIASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILDGNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATNLANLIQEKLS"
     gene            11005..12837
                     /gene="glmS"
                     /locus_tag="MJE33_001034"
     CDS             11005..12837
                     /gene="glmS"
                     /locus_tag="MJE33_001034"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBY9646993.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEKEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELISEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIILDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKEIPSKIQEIL
                     DNEEYIKEVAKTVVSSEHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAAGELKHGT
                     IALIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAADKIIYI
                     PNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
     gene            13289..14584
                     /locus_tag="MJE33_001035"
     CDS             13289..14584
                     /locus_tag="MJE33_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase"
                     /protein_id="HBY9646994.1"
                     /translation="MTNDFKKEKKEDYFVNLKAISTEVLQEKVQDNAWVIVDTRLNDA
                     YNGWKLDGVKRGGHIKGAVDFSANWLSVYSDRKDEVLEQALKTKRIDLDKNIVLYDAN
                     GKDALVVANYLSKKGYKYLYKYDIKQWADDENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFEDSKNIKMIEASWGKESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLTKFALD
                     YGLTKDDTVIVSSSTPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFISNPK
                     HSCTNFGADIPVNSQLIVTTSELRQKLKEKNKFILVDNRTWDEHIGKVSGYTYYDKKG
                     RIPGALYGHSGSDSVSLEEYRNIDNTMRNKYEILEMWDKENIDVNKQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
     gene            15012..15749
                     /locus_tag="MJE33_001036"
     CDS             15012..15749
                     /locus_tag="MJE33_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HBY9646995.1"
                     /translation="MKKVKLAISFFILMIIGMTISYADTSINTRYNRLSKALEISEAN
                     FKFYNVKINSVINHNLSNKEFHNIFIEIINNLKLDKKGINWNQKGSGDKIQLCATIKG
                     SDKEISIKGINKNNKESYIVVDILDNKVYKHKEVIYSNVENVLNKYSSQVDVSACIVG
                     EYTKRLQNNKYDDILQKILYNMSAKQIDEVKDVNFLSITAYSKVLNDYELEYLGEKIN
                     LNIGIKYNEDDGKTLIYIATPIIKLDY"
     gene            15770..17023
                     /gene="murA"
                     /locus_tag="MJE33_001037"
     CDS             15770..17023
                     /gene="murA"
                     /locus_tag="MJE33_001037"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBY9646996.1"
                     /translation="MAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGKSTLNGV
                     PNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFLVMGPLL
                     ARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGNKLYLDF
                     PSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNTIKIKGV
                     KELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAGCEITEM
                     DNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFENRFMHV
                     AEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGETQIGEIY
                     HIQRGYVDIDKKITALGGQIEIVED"
     gene            17110..18174
                     /gene="spoIID"
                     /locus_tag="MJE33_001038"
     CDS             17110..18174
                     /gene="spoIID"
                     /locus_tag="MJE33_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HBY9646997.1"
                     /translation="MKNPLVVLLGFVTCSVLVPSLITLVSYKNVELTEKPESPVSINK
                     TIKKSDIEDKGNKEEKNTVNYETVNKKAPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIITYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            18240..18908
                     /locus_tag="MJE33_001039"
     CDS             18240..18908
                     /locus_tag="MJE33_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HBY9646998.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFDDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVSVKKEQKVTEGQSLGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            19006..19266
                     /gene="spoIIID"
                     /locus_tag="MJE33_001040"
     CDS             19006..19266
                     /gene="spoIIID"
                     /locus_tag="MJE33_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HBY9646999.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEKEHKKM"
     gene            19463..20482
                     /locus_tag="MJE33_001041"
     CDS             19463..20482
                     /locus_tag="MJE33_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBY9647000.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDKRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            20498..20926
                     /gene="fabZ"
                     /locus_tag="MJE33_001042"
     CDS             20498..20926
                     /gene="fabZ"
                     /locus_tag="MJE33_001042"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBY9647001.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNVTVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEVEMTTLRKNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(21037..21546)
                     /gene="yyaC"
                     /locus_tag="MJE33_001043"
     CDS             complement(21037..21546)
                     /gene="yyaC"
                     /locus_tag="MJE33_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HBY9647002.1"
                     /translation="MYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDRAIGDTL
                     GPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDACLGSQSN
                     IGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFILDLAET
                     IALALLVST"
     regulatory      21735..21838
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            21975..23168
                     /locus_tag="MJE33_001044"
     CDS             21975..23168
                     /locus_tag="MJE33_001044"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBY9647003.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEETKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     gene            23484..24581
                     /locus_tag="MJE33_001045"
     CDS             23484..24581
                     /locus_tag="MJE33_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647004.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     IGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGTFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMAIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSDIGNIYTKEIPALYLADKISPIIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCMLYRRITKTEGYKENKSEIS"
     gene            24845..27064
                     /locus_tag="MJE33_001046"
     CDS             24845..27064
                     /locus_tag="MJE33_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBY9647005.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKIYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIEVINKNPYQLAEDIRGIGFKVADSIASKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELNGEIIKECVMMLVYNQKIHIEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEEDKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMINSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVDKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEESFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYILFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPIAWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            27067..27849
                     /locus_tag="MJE33_001047"
     CDS             27067..27849
                     /locus_tag="MJE33_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBY9647006.1"
                     /translation="MKICNDNFIKNFKNMINKCLDFIYPENISCIICDKSIKKTNTYS
                     ICKSCFKEMNFIQDGCMKCGKPIICHSIEKEFIEECSYCFNKDFYFDKSISCIEYNDV
                     TKKMVLGLKYNQKTFMAKYIAQIMKEKLYLENIKFDYILFVPLHKKRLNKRGFNQAQK
                     IAFNLSKIVNIPLLDCISRKKYTRMLYKLNKKERKEELKNVFVVKENVKLINNKNILL
                     IDDIFTTGLTTNEISKLLKLSGANKVFVLTLLTKANDNYIME"
     gene            28181..30094
                     /locus_tag="MJE33_001048"
     CDS             28181..30094
                     /locus_tag="MJE33_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBY9647007.1"
                     /translation="MICLNKRQENLLTTLLLKEEFINVNKIANDFECSERTIRNDCKY
                     IDEWLSTFCNAYIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLL
                     LCNNKKVTLNDLSNKLYINKNIVREEISKLSVALNNYNLNISTKKGSGIYIEGDEKDI
                     RIMLVSFLFKYIDKYRLNIDEIEYFHIVDIIIVKNIMLTIEEDMDIRLTDISFKQLML
                     FLLINIIRIRLGNSLKVSNTKKELKLKMLILDIEDELKSNLSIVLNYEEKLFIESLIL
                     GMNKQINSNNIKKSLYLNEELASYTKKIINLVSEESGINFNNDILLYEQLICHLNVTM
                     HQMKSNVYLENPLLDNIKTKFCFLFTVISSSIEIELKGKQITEDEIGYLTLHFQTSLE
                     RKYSKRESNKKASIVCPFSFGVSMLLKVKIEKRFDNIKIIETLREEDLKRDNFNKTID
                     FIIAFQEYENIQKPIFITTPLFTDEDENKLKEFTNKIKEKDTSYKLMNRLMMSDYLIR
                     ELEHDDIYEAITYLTNLLIEKHYAEKEYLQSIITREKSYPTNVGKGILFPHGDMKYIK
                     QSVLCFARLKTPIKIRNGKDIKIILLLAYKKDDDRKVFRELFKEISNLTEDENMLDIL
                     SKCDIEKVKDILI"
     gene            30235..30543
                     /locus_tag="MJE33_001049"
     CDS             30235..30543
                     /locus_tag="MJE33_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBY9647008.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            30566..30892
                     /locus_tag="MJE33_001050"
     CDS             30566..30892
                     /locus_tag="MJE33_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY9647009.1"
                     /translation="MKRKVYLFCSFGMSTSLLADKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            30931..32265
                     /gene="celB"
                     /locus_tag="MJE33_001051"
     CDS             30931..32265
                     /gene="celB"
                     /locus_tag="MJE33_001051"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBY9647010.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLVSSPLLIIGSLFLLV
                     ANFPIKGWNEFVGQIFGPDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIITPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGSIHEFIFKFL
                     QMPLLKLGNSLGAMAIAYIFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGS
                     KIPNIITGQFQDMFATFGGAGSTLSLVIAMLLICKSKRIKSMGKLSILPGIFGINEPL
                     IFGLPIMLNPILLIPFAIVPTINIIIAYFCMSAGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQLVLLILGIFMYIPFIKMLDKQYLREENETVEEEDDDISFDDLVL"
     gene            32276..32797
                     /locus_tag="MJE33_001052"
     CDS             32276..32797
                     /locus_tag="MJE33_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HBY9647011.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGVMMEQFLNE
                     SKVIATLFCGDEFFVNNQEEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKTDIPAFAAMSEENIDVINEYKNDICIVKTPKKGGIGLNDSLNNICKLAKAIANK
                     EDITLMKEEFCYQ"
     gene            32890..33849
                     /locus_tag="MJE33_001053"
     CDS             32890..33849
                     /locus_tag="MJE33_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HBY9647012.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIERRGKAGDLSLVAMNNKGEWGVATNIADFTFSVV
                     TKEESPTVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            33898..34950
                     /locus_tag="MJE33_001054"
     CDS             33898..34950
                     /locus_tag="MJE33_001054"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBY9647013.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVKGESENGFPFGEKVGE
                     ALNKALEISEKLGFKVRNLDNYIGYAEHGDSDDYVCVIGHVDVVHEGDGWKHQPYKGE
                     ETNGRIYGRGVLDNKGPIMSALYGLYAIKELNLKLDKRVRIIFGTNEESGFEDIPYYL
                     EKEKAPIMGFTPDCKYPVVYGEKGMAKIRIKSKINYEEDVYLGFIENMSENVLVTYKE
                     LNIENSDTILDIKVKYDFSYKLKDVLDEIKASFPNSIDIEVISNFNPVYFDKESNLVK
                     KLQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            34953..35627
                     /locus_tag="MJE33_001055"
     CDS             34953..35627
                     /locus_tag="MJE33_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBY9647014.1"
                     /translation="MLEIIGMSVEDAKIIEDCGADRIELVSALTEGGLTPSFGLIESV
                     VNSVKIPVNVMIRHHAKSFVYSKEDISIMQKDIGVVKEIGANGVVFGVLDKNNNIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKILKDYDKITNILTSGGKGSIVDNIQMIK
                     NMMLSSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRINNSPFEDIDRQKLKQL
                     VNIISR"
     gene            36180..36731
                     /gene="raiA"
                     /locus_tag="MJE33_001056"
     CDS             36180..36731
                     /gene="raiA"
                     /locus_tag="MJE33_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBY9647015.1"
                     /translation="MNIIISGKQIELTDAIKNKIETKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDAEINLADEQEDYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
     gene            36863..39538
                     /gene="secA"
                     /locus_tag="MJE33_001057"
     CDS             36863..39538
                     /gene="secA"
                     /locus_tag="MJE33_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBY9647016.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMIREDLPDCVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPTSKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            39553..40660
                     /gene="prfB"
                     /locus_tag="MJE33_001058"
     CDS             join(39553..39627,39629..40660)
                     /gene="prfB"
                     /locus_tag="MJE33_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153309863.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBY9647017.1"
                     /translation="MLKLQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNQQ
                     DFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDNDSVERDIEKS
                     IESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
                     YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFA
                     SIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQN
                     ERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLV
                     KDHRTNAEFGNVDSVMNGNIDLFINEYLKMNKIV"
     gene            40804..42945
                     /locus_tag="MJE33_001059"
     CDS             40804..42945
                     /locus_tag="MJE33_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBY9647018.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLENLALSILENNLDNIEIEAKNYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYSEAVKSMAPHRVL
                     AINRGEKESFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVSSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DSEVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHVAGISKAIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNIGEIDEKIKEIGLRELSEKLEVGQVTLKDIIAEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMTLKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            43093..44259
                     /locus_tag="MJE33_001060"
     CDS             43093..44259
                     /locus_tag="MJE33_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBY9647019.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIDEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPIDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTCGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIVDELVEEGFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKALGIEDRVGSIEVGKDADLVIWDNSPLEIQSNVLYTIINGKVVYEKK"
     regulatory      44319..44436
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            44561..45166
                     /locus_tag="MJE33_001061"
     CDS             44561..45166
                     /locus_tag="MJE33_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBY9647020.1"
                     /translation="MQNTAKRGNMISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFL
                     KIDLSDIPAIFGGMSLGPLVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMIL
                     CYSYKRSGDLKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINP
                     QVHDLFTFVIWMIAPFNVLKAVIISLVTLPLYKKMGNIIKK"
     gene            45495..46535
                     /locus_tag="MJE33_001062"
     CDS             45495..46535
                     /locus_tag="MJE33_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBY9647021.1"
                     /translation="MHLSKKIKKLFLDDKGYYFNSNWKDIHIGIYDTKLGDSLKYITN
                     EYIENMERVKYDYRELKNIENQTDGMCILAIRQNYKALEYVKNQTEELCLEAIKYNYK
                     ALEYVKEQTEYLCLEAIKKDCNALKYVRNKTEGLIIKAISHSSNIDVVSILKALETQT
                     RRICLEAIKKDGRCLAYVREQSEELCIEAIKQNYKALKYVKNQTEKMCIESVRQNGMA
                     LQYVNKQTDKICIEAVKQDGRSLQFVNNKTEEICINAIRYLNKKYNIKDVLSYIDKYT
                     EDICIEIVRQNGKMLMYIKNQTEKMCIEAVKENYKSLKYVKEQSERICKEALKQNHKA
                     KEYVKIAIDDCI"
     gene            46674..47126
                     /gene="tsaE"
                     /locus_tag="MJE33_001063"
     CDS             46674..47126
                     /gene="tsaE"
                     /locus_tag="MJE33_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBY9647022.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIEDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELRYKDEGREMILTPKGEFYKEIVEELIK"
     gene            47126..47842
                     /gene="tsaB"
                     /locus_tag="MJE33_001064"
     CDS             47126..47842
                     /gene="tsaB"
                     /locus_tag="MJE33_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBY9647023.1"
                     /translation="MKILGMDTSSMAASVAVVEDDNLICEFTVNNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     INFCDRKICCILDAQKNQVYSAKYKFENGEMIELDGVDVVEFDALVDEIVSTNEEFII
                     VGEAVYKYKDKLENIKNIKIPSPANNVSNAGSLCSLALNKYNKNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            47832..48308
                     /gene="rimI"
                     /locus_tag="MJE33_001065"
     CDS             47832..48308
                     /gene="rimI"
                     /locus_tag="MJE33_001065"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBY9647024.1"
                     /translation="MEDNTKLIVDDIKIEEMTTEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLNGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKGIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            48314..49330
                     /gene="tsaD"
                     /locus_tag="MJE33_001066"
     CDS             48314..49330
                     /gene="tsaD"
                     /locus_tag="MJE33_001066"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBY9647025.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREVLSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVVQEALDKANIGFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDNLAKNGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSTKALKAVKDKGYNIITLSGGVAS
                     NSGLRAKITELAKDNGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            49627..52335
                     /gene="hpdB"
                     /locus_tag="MJE33_001067"
     CDS             49627..52335
                     /gene="hpdB"
                     /locus_tag="MJE33_001067"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HBY9647026.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLSEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKAAFSHMTPNIIPGEKIVMQ
                     KTRHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIEMAKECKKAVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVQILEHIAHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLKEDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKLPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDYRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSIINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEMKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATPDGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
     gene            52339..52596
                     /gene="hpdC"
                     /locus_tag="MJE33_001068"
     CDS             52339..52596
                     /gene="hpdC"
                     /locus_tag="MJE33_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HBY9647027.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDAACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            52589..53539
                     /gene="hpdA"
                     /locus_tag="MJE33_001069"
     CDS             52589..53539
                     /gene="hpdA"
                     /locus_tag="MJE33_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HBY9647028.1"
                     /translation="MSSQKQLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYENGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKPYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNIHTAIETSACVSNEVFNKIFNDIDFAFIDIKHMDREKHKEQTGVYNDLILENIS
                     NLANSDWNGRLVLRVPVISGFNDSDENISDIISFMHKNNLVEINLLPFHRLGESKWIQ
                     LGKEYEYSDKGDVDEGHLEELQDIFLDNGIACYVGHETAF"
     gene            53854..54816
                     /locus_tag="MJE33_001070"
     CDS             53854..54816
                     /locus_tag="MJE33_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBY9647029.1"
                     /translation="MNKSWSNLIIKNKDRKVRSGWIIIAVMATFYILQYYLSMILVEI
                     IRKILVATGDINLKTNYFSEYANWLNDVGLQIVFQILTDSLMIIIPIIVWRFIMKNPI
                     SEMGLHSYRIKKKEGYVGMLLGIFNCTVIFIITITIGGGHVTSWVPKISPLTVSWILV
                     FILVAFAEEILNRGFFMAVLRRCKNIYFIMIVPSVIFGLIHIWNPDVTFLSVINIIII
                     GILFSYMFIKSSNIWMCIGYHFAWNVFQGIIYGMPVSGLQVPGIITTQITHGNLLNGG
                     MFGIEGGILTTFVNLLSFIFVWYYYRDSKYDFVSDKVNVTNIKN"
     gene            complement(54915..55718)
                     /locus_tag="MJE33_001071"
     CDS             complement(54915..55718)
                     /locus_tag="MJE33_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBY9647030.1"
                     /translation="MMILPLSSVAIAKFYTEGLNNDMYEFYSLIILFFLIYSLLIVLK
                     LLNLINNNEFIIINLILTFISSLSIIIYSSNKKNLYPVKNIKLGSLLIIYFMISVIVP
                     TSFILILQGAQPNYSGIIKFIFTPITSLTTIYIFFSEEYAWRGFLQNIFFDKFGKKLG
                     VIILGMCWSLWHLPLIFTLYTPEAPILGIILRSIHIVGISIFLGYLYIKTKNIWFCAI
                     IHVLNNSAFLITNSKESTITYHDILIVSMVVLIFYVPFLFTKEYKNNLE"
BASE COUNT        22432 a         6001 c        10575 g        16875 t
ORIGIN      
        1 gtggagcagt tggcagctcg tcgggctcat aacccgaagg tcgtaggttc aagtcctgcc
       61 tccgcaacca ttaaaacggc tcgttggtca agaggttaag acaccgccct ttcacggcgg
      121 taacaggggt tcaattcccc tacgagtcac caattcaatt aaatatgcgg gtgtagctca
      181 atggtagagt tctggccttc caagccagct gtgagggttc gatccccttc acccgctcca
      241 aaaatgggac catagctcag ctgggagagc acctgcctta caagcagggg gtcacaggtt
      301 cgagccctgt tggtcccacc attcgcggcc tggtagttca gttggttaga atgccagcct
      361 gtcacgctgg aggtcgaggg ttcgagcccc ttccaggtcg ccaaattaaa taagcgggaa
      421 tagttcagtg gtagagcgca accttgccaa ggttgaagtc gcgagttcga atctcgtttc
      481 ccgctccata ataacttggg tgcatagctc agctggatag agcaacgccc ttctaaggcg
      541 tgtgtccggg gttcgaatcc ctgtgcgctc attaagaaaa gtttactata aattatagta
      601 aacttttata ttgtttaaaa agacacagga aacatgtata ttacctgtgt ctttttattt
      661 ataaacttta aactatgtaa aaattacagt atattctaat agagttacag tatattcatt
      721 aatattgtaa acatacggag ggtaatgtat aatagtctac aagatgttta tgaaggcatc
      781 agttaaatat aataaatata actaattagt aagagttata tacattatat tgtatacttt
      841 atggtattta tatattgaaa taacttatga aattatttat aaagagattc atgagcatat
      901 ttttgtctaa acaataagta taagaaatta taaaaaatta gagactatag tgaatttaca
      961 atttaatgta atatttataa gttatactaa ataaattatt atactgtgaa atggatatat
     1021 aactttaggt attgtgtatt attttcactg taggagatta tatctagtta tacataataa
     1081 gttttaatga tagtttatag taagatatta aaataaattt atctaatcta gatatgaaat
     1141 aagttattac tttaatataa aaatgaatct aatggaataa tattgatgat aagtaatatt
     1201 tagattttgt atttgtttat agttaataag tggattatag aatttataat aattaatata
     1261 tattaaacaa agatatatta agatatttta gaacataaaa ctgaattaag gcgcgagtac
     1321 tattttgtat tcgcgccttg atggtttgag ttatttgagt aaaaactaac tttaataaga
     1381 aatatgtatt tattaagttg atatttttaa tgtgtttaga gattatttaa caatctccaa
     1441 atataaaact agaattattt acataaaagt taaatagttt gtatagagtt agatttatat
     1501 agtataatac ttagtgtaaa taattaagaa gttatcaaac aggaggataa aactgtgtta
     1561 gatataaatt cttttttaaa tggatttttt aatataatat taagaatgag tatatatgat
     1621 ttaattgaca tatctatagt agcgtatatt ttttacaaaa tatttatgtt tataaaagat
     1681 acaagagcag aacaagtgtt taaaggtata atatttcttt tattagcaac tcaacttagt
     1741 aacaccttta aattgcatac cgtttattgg atatctctta aagctttaga ttatggagtt
     1801 atagctgctc ttataatttt tcaaccagaa tttagagcag gacttgaaca tataggaaga
     1861 gcaaagttta atttatttgg aaagaatgta aatacttctg aagaaactct caatagaaat
     1921 atagaagaaa ttgtagaagc tttatattca ctttctagac aaaaaatagg agctcttatc
     1981 ataatggaga gagaaactag aataagtgat ataataaata caggaactat tattgatgct
     2041 gaaatttcta ggcaactatt aataaatata tttataccaa atacaccatt acacgatgga
     2101 gctgttgtaa ttagagattc taaggtaaaa gcagctgcat gttttttgcc attgacagaa
     2161 agtaaggatt taagtaaaga tttgggcact agacatagag caggaatagg ggtaagtgag
     2221 gtgtctgact gtataactct gattgtatca gaggaaacag gtggcgtatc aattgctaaa
     2281 gcaggtaagt tatatagaga tatttctaga gaaagaatga tgaatatatt acgtagtaat
     2341 ttaaaaacaa atacagaaac aagaagtttt ttcaaaggtg gtatatttaa atgataaata
     2401 gactaaaaaa taataccaaa ataaaaataa tatcattttt aagtgcaatt gcattatgga
     2461 tgtatgttat ggctgtagtt gaccctgaag aaactaaagg gtttgaagat atacctgtat
     2521 ctataagtaa tatgagtgat ttaaaggata aaaatcttgt tatatatcca gacgaaaaat
     2581 taacagcaga tgtatatata aaaggtagtt tatcaaaggt acaaagtgtt aaaaaagaag
     2641 atatacatgt atatggaacc attgaagacc caatacaagg acagaaggaa gtatatttaa
     2701 aagcaaatat tcctcaaggt gttactctag aatttaagca tgatgtttta gtagtaaatc
     2761 ttgaaaaaaa cataaaagaa aaaagagata taaaggtaga agtaagtgga aattcaaaaa
     2821 gaaatataaa taaaattgaa cttagtaagg aatttgtcga tgtatcaggt ccaagaactc
     2881 tagttaaaga agtcaaaagt gtaagagcta atctagatgt tggtaatgag acagatgatt
     2941 tttctaaaaa attgcaatta ataccattaa atagtgatgg aactaaggtt gaaggagtaa
     3001 cattaagttc atcctttgtg actgcaaaga tagagctatt gaaggaaaaa attgtcccaa
     3061 taagagtaag tctaaatgat aataaagata atgatgtact gaaaaactat aaattaagta
     3121 aggaacaagt tacaataaaa ggaaagaaag atgatataga caaaataagt tatattaaga
     3181 caaaacctat tgacttagct gatttggcta atggtgaaga aaaagaagtt agcttagaaa
     3241 ttccagaggg tataactctt gaggatagct ctataacaat taaattaaat gatacaaaac
     3301 aattgagtgc agaattttta tataatgcgg gagatattga attaagaaat atcccaacaa
     3361 atatggataa tccaaatgta atttctgatg tagatataaa agttgttgta gagtcaacgg
     3421 aggatttgag taagttaaat aaaaaagata tttcattata tattgattta tcacaaggtc
     3481 ctgaggaaga tagtaaatat aaaataaaat atgaaacaac atatcaattt aaaaaaataa
     3541 atatagaacc taatattgtt gaagtggaat gaacaaatat ttcgaatatt tagttgttac
     3601 aagatgcgac aaaaaaacct ataaaattaa gtttgttaat tttatgtttt tgttgcatct
     3661 tgtttatttt agttatatta taaaataagg acaatgagat atttgttaaa tctaaaaagc
     3721 gctagatata tgaaaaaaga ctgataaata gatttaaata aatattttga attaaatatt
     3781 tcaattatct ctaggaaaac gttgctgaac gagttataat ttaattgaga tataaaaagt
     3841 tctatttcaa aaaattcaag atgaggtgga aatgcatgag aagttttgaa gaagtaatta
     3901 agtttgcaaa agaaagagga cctaaaacta tatcagtagc atgttgccaa gataaagaag
     3961 ttttaatggc agttgaaatg gctagaaaag aaaaaatagc aaatgccatt ttagtaggag
     4021 atatagaaaa gactaaagaa attgcaaaaa gcatagacat ggatatcgaa aattatgaac
     4081 tgatagatat aaaagattta gcagaagcat ctctaaaatc tgttgaatta gtttcacaag
     4141 gaaaagccga catggtaatg aaaggcttag tagacacatc aataatacta aaagcagttt
     4201 taaataaaga agtaggtctt agaactggaa atgtattaag tcacgtagca gtatttgatg
     4261 tagagggata tgatagatta tttttcgtaa ctgacgcagc tatgaactta gctcctgata
     4321 caaatactaa aaagcaaatc atagaaaatg cttgcacagt agcacattca ttagatataa
     4381 gtgaaccaaa agttgctgca atatgcgcaa aagaaaaagt aaatccaaaa atgaaagata
     4441 cagttgaagc taaagaacta gaagaaatgt atgaaagagg agaaatcaaa ggttgtatgg
     4501 ttggtggacc ttttgcaatt gataatgcag tatctttaga agcagctaaa cataaaggta
     4561 taaatcatcc tgtagcagga agagctgata tattattagc cccagatatt gaaggtggta
     4621 acatattata taaagctttg gtattcttct caaaatcaaa aaatgcagga gttatagttg
     4681 gggctaaagc accaataata ttaacttcta gagcagacag tgaagaaact aaactaaact
     4741 caatagcttt aggtgtttta atggcagcaa aggcataatt ttaaggggga aataggcaaa
     4801 tgagcaaaat atttaaaatc ttaacaataa atcctggttc gacatcaact aaaatagctg
     4861 tatttgataa tgaggattta gtatttgaaa aaactttaag acattcttca gaagaaatag
     4921 gaaaatatga gaaggtgtct gaccaatttg aatttcgtaa acaagtaata gaagaagctc
     4981 taaaagaagg tggagtaaaa acatctgaat tagatgctgt agtaggtaga ggaggacttc
     5041 ttaaacctat aaaaggtggt acttattcag taagtgctgc tatgattgaa gatttaaaag
     5101 tgggagtttt aggagaacac gcttcaaacc taggtggaat aatagcaaaa caaataggtg
     5161 aagaagtaaa tgttccttca tacatagtag accctgttgt tgtagatgaa ttagaagatg
     5221 ttgctagaat ttctggtatg cctgaaataa gtagagcaag tgtagtacat gctttaaatc
     5281 aaaaggcaat agcaagaaga tatgctagag aaataaacaa gaaatatgaa gatataaatc
     5341 ttatagttgc acacatgggt ggaggagttt ctgttggagc tcataaaaat ggtaaaatag
     5401 tagatgttgc aaacgcatta gatggagaag gacctttctc tccagaaaga agtggtggat
     5461 taccagtagg tgcattagta aaaatgtgct ttagtggaaa atatactcaa gatgaaatta
     5521 aaaagaaaat aaaaggtaat ggcggattag ttgcatactt aaacactaat gatgctagag
     5581 aagttgaaga aagaattgaa gctggtgatg aaaaagctaa attagtatat gaagctatgg
     5641 catatcaaat ctctaaagaa ataggagcta gtgctgcagt tcttaaggga gatgtaaaag
     5701 caatattatt aactggtgga atcgcatatt caaaaatgtt tacagaaatg attgcagata
     5761 gagttaaatt tatagcagat gtaaaagttt atccaggtga agatgaaatg attgcattag
     5821 ctcaaggtgg acttagagtt ttaactggtg aagaagaggc tcaagtttat gataactaat
     5881 gataaaagaa taagaatcat aactggtcac tatggaagtg gtaaaagtga atttgcgatg
     5941 aactatgttg ttaagttaag agatatggtt tctggcaaag ttgctatagc cgatttagat
     6001 gtggtaaatg tatactttag aactagagaa aagaaagagt taatgaaatc cttaggtata
     6061 caaccaatag atagctcaat taatgctcca acattagatt taccagcagt atcagcagag
     6121 gtaatgtctc ctatggtaga ccattcatac aattctgtta tagatttagg tggagataat
     6181 gtaggtgcta gggttatagg aagatttagt catttgttaa aagaaggcga ttatgatatg
     6241 ctttttgtaa taaatgcaaa tagggagaaa actcaaactt ctgaagaagt tatccaatat
     6301 ataaaagaaa tagaaaaatc ttctaaatta aaggtaacag gtctaattaa taatactcat
     6361 cttattaggt ttactactat tgatgatgtt ttaagaggtc agaaagttgc aaaagaagtt
     6421 tctgaaaaat gtaatatacc gataagatat gttgcatgtt tagaaaatct tgttgaacag
     6481 ttaccaaaag atttagaagg agaaattttt cctataaaat tatatatgag agaagactgg
     6541 atgtagtttg agctcaaata tatagaaagt ttaaggaggc ttaggtaaat ggctaaagga
     6601 aaagtatctt ttaatcaaga acgttgtaaa ggttgtggat tgtgtgttga agcatgtcca
     6661 gtaaaaatca tacaattaga ctcaaatgta ataaacaaaa aagggtataa cccagcaact
     6721 gtttttgaaa tggaaaaatg cataggatgt gcaagttgtg caactatgtg tccagatgta
     6781 gttataacag tagaaagaga ttaatttaat taatatttaa gggggaattt taatggctaa
     6841 gatacttatg aaaggtaacg aagcatttgg aaaagcagct atagaagctg gttgcaaata
     6901 tttcttcggt tacccaataa ctccacaaag tgaattacca gaatatttat caagagagtt
     6961 acctaaaata ggtggagcgt tcgttcaagc tgaatcagaa gtttctgcga taaacatggt
     7021 ttatggtgga gcaggtgcag gagcaagagt tatgacttct tcatcttcac caggagttgc
     7081 tttaaagcaa gaaggtataa catatgcagt aggagcagaa gttccttgtg ttgtacttaa
     7141 tgtaatgaga ggtggtccag gacttggaag tatacaacct tcacaagctg actactttat
     7201 gtctacaaga ggtggaggaa atggagacta cagaactcca gtattcgcac cagctacagt
     7261 tcaagaagct gttgatatga taatggaagc ttttgacgtt gcagattatt acagatcacc
     7321 agttatggta gttgcagatg gtatgatagg tcaaatgatg gagccagttg aatttagagc
     7381 tcctgagaag aaaagagaat tgcctcctaa agattgggca actgttggaa ctaaaggaaa
     7441 aagaaaacct aatgtaataa actctctata cttagagcca gaagtactag aagaccattg
     7501 ttggcattta caagaaaaat ttgatgcaat ggaaaagaat gaagtacaat acgaaatgta
     7561 caaaacagaa gatgctgaat ttgtatttgc agcttatgga actacttcaa gagtagtaaa
     7621 aagcgcaatt gatatattaa gagaagaagg aataaaagca ggtcttataa gacctaaagt
     7681 attatggcca ttcccattcg aagcattcaa ccaaattcct aatgctagaa acatattgac
     7741 tgttgaaatg agtatgggac aaatggtgga agatgttaaa atggcagtag aaggtaaatt
     7801 accagtttac ttccatggaa gaccaggagg aatgactcca acaccagcag aaatagttga
     7861 aaaagctaaa aaaattattg ccggagaatt ggttgcagga ggtgctagat aatggctgtt
     7921 gtatttaaga aaactgaagg attacaagat actcaaactc actactgtcc aggatgtaca
     7981 catggtataa tccatagatt agttggtgaa gtattagaag aactaggagt attaggtgat
     8041 gctgtaggtg ttgttccagt tggatgttca gttttaggat ataaatattt taactgtgac
     8101 actcaagaag ctgctcatgg tagagcgcca gctgctgcta caggaataaa aagagttcat
     8161 cctgaaaata cagtattcac ttatcaaggt gatggagact tagcttcaat tggtactgct
     8221 gaaatagttc atgcagctgc tagaggagaa aaaataacta ctatatttgt aaacaataca
     8281 acttatggta tgactggtgg acaaatggct ccaactacat tagttggaca aagagctact
     8341 actgctcaat caggaagaaa tgctgaaact caaggttatc caataagagt aagtgaaatg
     8401 ttagctacat taacaggagc tgtatttgta gaaagagtag cagttgatac tccagctcat
     8461 gttagacaag ctaaaaaagc tataaagaaa gcattccaag ttcaacaagc aggattagga
     8521 tttggaatag tagaagtatt atctacttgt cctacaaact ggggactagc tccaaatgat
     8581 gctttacaat ggttaagaga caacatgata ccatactatc cattaggaaa tttcaaaaat
     8641 gttgaagttg aggaggtgaa gtaaatgtct acagctagag taatatgtgc aggcttcgga
     8701 ggccaaggtg ttatgtctat gggacaatta cttacttatg caggaatgct agaaggaaaa
     8761 gaagtttctt ggttaccatc ttatggacca gaaatgcgtg gaggtacagc aaactgtgca
     8821 gtaactgtat ctaatgagcc agttggttct ccacttataa cagatgatgc aactgcagct
     8881 atattattaa atataccagc atttgaaaaa ttcaaagatg atgttattcc tggtggaaaa
     8941 ataatagtaa atagttcttt aataaaagaa aaagttgata gaacagatgt agatgtttat
     9001 tatataccag caaatgaatt agcagctgaa ttaggaaatg ataaagttgc taacatgata
     9061 atgttaggcg cttacttaaa agtatctgat actgtagata tagaatctgt actagaagca
     9121 ttcaaaaaag ttttcggacc tagaaaagaa aaattcgttc cattaaacag agaagcttta
     9181 caaaaaggta tggatgctac atgttctaaa gcaactaact aataaagaaa aagatgtcaa
     9241 aaaaaagaca atagaattat ttctattgtc ttttttgctg ataagtatta aaatattttt
     9301 atgactgaaa ttaagcctat aatgatattt tatgaactat aaaaatataa tacaaattac
     9361 aagcttttca attatgaaag gagaatcttt agtgagaaag tattttggaa cagatggagt
     9421 tagaggagta gcaaatacag aactaacatg tgatttagca tataaacttg gtagagctgg
     9481 tggatttgtc ttagctcaag gagaccacag agtaaaagta gtagtaggta aggatactag
     9541 aatatcaggt gatatgttgg aagcatcttt aatagcagga cttatgtctg ttggatgtga
     9601 tgttattact gttggaataa taccaacacc tgcagtggca tatttaacta gaaaatatgg
     9661 tgctgattgt ggtgtggtta tatctgcatc tcacaatcct gttgaatata atggaatcaa
     9721 attctttaat aagaatggat ataaattaga tgatgaaatt gaattgaaaa tagaagaata
     9781 tatagacgat atagacaaaa ttgattgtct tccaattggt gaaaatgtag gaagaaaatt
     9841 acatgaacat tgtgcacaaa gagactatgt agactaccta aaatcaataa taagcactga
     9901 ctttaaggga ttaaaagtag ttttggactg tgctaatggt gcgtcatata aagtggcacc
     9961 aatagtgttt gatgaactag gagcaagtgt aatttcaata aatagttcac cagatggaaa
    10021 taatataaac tataaatgtg gctctacaca tccagaacaa cttcaaaggg ctgttttgga
    10081 acataatgct gacttaggtc ttgcatatga tggagatgct gatagattaa ttgcagtaaa
    10141 tgaaaagggt caaattgttg atggagacca tataatgata ttgagtgcct taaatttaaa
    10201 gaaaaataat aaattggcac aagatacttt ggtagttaca gttatgagta atatagggtt
    10261 gacaatagct gctaaggaaa atggaataaa tttatctact acagcagttg gagacagata
    10321 tgttttagaa gatatggtta aaaatggata taatcttggt ggagagcaat ctggacatat
    10381 gatattttta gattacaaca ctactggaga tggagtgctt agttctctaa tacttgcaaa
    10441 tataatcttg caagaaaaga aacctttatc agaaattgca tcaataatga gccaataccc
    10501 acaagtatta gtaaatgcaa caataaaaaa tgaaaataag aacaaatata tggaatatcc
    10561 agaaataaaa actgagatag aaagaataga atctatttta gacggaaatg gtagagtttt
    10621 gataagacca tcaggaactg agccattagt tagagttatg ttagagggta aagaggaagg
    10681 tcaaataaaa gaattagcaa caaacttagc taatcttata caagaaaaat tatcgtaatt
    10741 taaagggtat tctcagatat aaatactgag gacacctttc taagcgccag aacttagtta
    10801 tagacataat tattgagtaa tataggaact aagttgacga ggtcagagtt aatcgaattg
    10861 tcggcggatg ctctgcggtg tgtttaccat cgaaagactt ctttaaataa taggagcaat
    10921 cctatggaca aagggaagcc aaagtaaact tatatgacct aactcttaaa atatataaat
    10981 aatattttta ggaggacaca aataatgtgt ggaatagttg gatatttagg aagtagaaag
    11041 gcagcagaag ttatagtaga agggctttct aagctagagt acagaggtta tgactcagca
    11101 ggagtagcag ttaatagttc taatgaaaaa gaacttaata ttagaaagtt taaaggtaga
    11161 ctttctgtac ttgcagaaga cttagaaaag aatcctattg atggaaattt aggtataggg
    11221 catacgagat gggcaactca tggagaacca tctgatgtaa actcacatcc tcattttaat
    11281 caagccaaaa caatagcagt tgttcataat gggataatag aaaactatat ggaaataaaa
    11341 gaagaattaa tttctgaagg tgttaagttt gaatctcaaa cagatacaga ggtaatagca
    11401 catttagtag ataaatacta tgagggtaat ttattagatg ctgtatacaa aactatatca
    11461 aaattaagag gagcttatgc attaggtgtt atatgtaaag aacatggaaa tgaattagtt
    11521 gcagtaagaa aagatagtcc tcttgtagtt ggtgttggtg aaggtgaaaa ctttatagca
    11581 tcagatatac ctgcattatt aaaatatact agagatgttt atttcttaga aaatggagaa
    11641 gtagttcatc taaaagacga aaatgtaaca gtttatgact ctaatagaaa tcttgttgaa
    11701 aaagaagtat ttcatgtaac ttgggatgta gaagctgcgt ctaaaggtgg atatgactac
    11761 tttatgtcaa aagaaattca tgagcaacca acaggagtaa gagaaacttt agaaagaaga
    11821 cttgatgata atggaaatat aattctagac agtataaata tttctaaaga agacttagaa
    11881 aaaataaata aagtttatat agtggcttgt ggaacagcat ataatgctgg attacttggt
    11941 aagtatgcca tagaaaaatt tgtaaatata ccagtaataa ctgatatagc atcagaattt
    12001 agatatagcg acccatttgt agatgaaaat tcattagtaa tattagttag tcaatctggt
    12061 gagacagcag atactctagc agtattaaga gattcaaagg ctaaaggagc aagaatactt
    12121 tcaataacta acgttgtagg ttcatctata gcaagagaat cagatgatgt attctatact
    12181 tgggcaggac ctgaagtagc agttgcatca actaaggcat atactactca aataacttct
    12241 ttatacatga tagctttaga ctttgctatc aaaaaaggaa caataactag agaattttat
    12301 gatagtatga tttctaagat gaaagaaata ccatcaaaaa tacaagaaat actagacaat
    12361 gaagaatata taaaagaagt tgctaaaaca gttgttagtt ctgaacatgc attctattta
    12421 ggaagaggta tagactatag cttagcaatg gaaggttcat tgaaattaaa agagatttca
    12481 tatatacatg cagaagcttt tgcagcagga gagctaaagc atggaacaat agctttaata
    12541 gaaaaaggaa ctcctgtaat tgccatagct actcaagaaa agttatttga aaaaatggtt
    12601 tctaatatgg aagaagtaag agcaagagga gcttatgtag tagcaatagc tcaaagccat
    12661 aataaggatg ttgagaaagc tgctgataaa ataatctata taccaaattc agatgatatt
    12721 ttatcaccaa tattagcagt tgtaccaatg caattacttg cttatcatgt ctctgtacta
    12781 agaggatgcg atgtagataa gccaagaaat ctagctaaat cagtaacagt tgaataaaaa
    12841 cagattaaaa attaaataga taatatttat tgaaaagaac tcatcctgtt atttgtattt
    12901 tataggatga gttttttaga taaatattaa atttgttttg tttttgctat aataaatttt
    12961 ataaaaagat tgatattaaa tcatctgtaa aatgataaat atatagacca taccaggtat
    13021 agggaacgat aaatatagtt catataaaat atagtgagta cttaataaac atatagtcaa
    13081 tatcagataa aaaaaataat agcttatgag aaaaattaaa atacaaggta ttagtaacct
    13141 tgtgataaga taaagtgagt agtttaaaat aagaatataa ggaactattt ataaaagaat
    13201 ataatgaagc taatagagta taagaaaatt actttatttt agtaaaataa tttcaaataa
    13261 aaattttttt aaattgggag tataaattat gacaaatgat tttaaaaaag aaaagaaaga
    13321 agattatttt gttaatctta aggcaatatc tactgaagtt ttacaagaaa aagtgcaaga
    13381 taacgcttgg gttatagttg atactcgctt aaatgatgct tataatggct ggaagttaga
    13441 tggagtaaaa agaggtggac atataaaagg agcagtagat ttttctgcta attggttaag
    13501 tgtatactca gatagaaaag atgaagtgtt agaacaagca ttgaaaacta aaagaattga
    13561 tttagataaa aatatagtat tgtatgatgc aaatggaaaa gatgctttgg ttgtagcaaa
    13621 ttatttatct aaaaaaggat ataaatatct atataaatat gatattaaac aatgggcaga
    13681 cgacgaaaat cttccaatgg aaagatataa aaattatcaa atgatagttc ctgcttttat
    13741 aataaaagat atattagatg gaaaaattcc tgagacattt gaagattcaa agaatataaa
    13801 aatgattgaa gcgagttggg gaaaagaatc gtatacaaaa gggcatatac ctacaagtgt
    13861 tcatgtaaat actgatatta tagaacctcc tccaacatgg atgcttgata atgatgataa
    13921 cttaactaag tttgctttag actatggatt aacaaaggat gatactgtaa tagtttcaag
    13981 tagtacacct atggcatctt atagactagc tgttatattg agatatatag gagtaaaaga
    14041 tgtaagagtt ttgaatggtg gaacaaactc atggttatca gcaggatatg aattagaatt
    14101 tattagtaat ccaaaacatt catgtactaa ttttggagca gatataccag ttaactcaca
    14161 attaattgtt actacatctg aattaagaca aaaactaaaa gaaaaaaata agtttatact
    14221 agtagataac cgtacttggg atgaacatat aggaaaagta tctggatata catattatga
    14281 taaaaaaggg cgtataccag gagctttata tggacattca ggtagtgata gtgtttcact
    14341 agaagaatat cgtaatattg ataatacaat gagaaataaa tatgagattt tagaaatgtg
    14401 ggacaaggaa aatatagatg tgaataaaca gcttatattt atgtgtggaa gtggatggcg
    14461 tgctgctgaa gtattaacat atgcaaatgt cataggagtg gaaaatacct ccctttatag
    14521 tgatggatgg atgggctgga gtcttgataa ttcaaattta attgaagtgg gagaacacaa
    14581 ataaagtttt tgaaagtgca atagagttgt ttaaaacaaa ggtgtttttg atttattagg
    14641 aagagtgtgt aaattgtgta ttgggaagtg tacttaggta tgttcatttg gagctatgga
    14701 aattgacaaa aaagtattat ctgtttattt tgttggtaaa atatgaatag attatagatt
    14761 taagtataga ctaagtaatt tatattacag aagagaatat ataagaatta atgcaagatg
    14821 tatttgatac atataagaaa ttggcaagtc caggagaagg atagagaaaa ttgagattga
    14881 agagtttgaa agagattatt agatgaaaaa tacaaaaatg tataaaaata catattgaga
    14941 aagtatatct ctcttaaatt tgttaataat tctattatac agaaaaacat atttaagggg
    15001 gattttttac tatgaaaaaa gtaaaattag ctataagctt ttttatatta atgattatag
    15061 gaatgacaat atcttatgca gatacaagta taaatactag atacaataga ttaagtaaag
    15121 ctcttgaaat atcagaagca aattttaaat tttataatgt taaaattaat tctgttataa
    15181 atcataattt atcaaataaa gaatttcata atatttttat agaaataatt aacaatctaa
    15241 agttggataa aaaaggaata aattggaatc aaaaagggtc tggcgataaa atacaattat
    15301 gtgctactat aaaaggtagc gataaagaaa tttctataaa aggaataaat aaaaataaca
    15361 aagaaagtta catagtagtt gacatattgg ataacaaagt atataaacat aaagaggtta
    15421 tttatagtaa cgtagaaaat gtgttaaata aatattcatc tcaagtagat gtgtctgctt
    15481 gtattgttgg agaatacaca aaaaggttac aaaacaacaa atatgatgat attttacaaa
    15541 aaatattata taatatgagt gctaagcaaa tagatgaagt taaagacgta aactttttat
    15601 ctataacagc atatagtaaa gtattaaatg attatgaatt agaatattta ggggaaaaaa
    15661 ttaatttaaa tataggaatt aaatataatg aggatgacgg aaaaacactc atatatattg
    15721 ccacaccaat tataaaatta gattattaat tatgaggaga tcaaaaaata tggctaagat
    15781 aatagtaaaa aagagtaatc cacttaaagg tagtgttaaa atagatggtg caaaaaatgc
    15841 agttttacca ataatagcag cgactttatt agcaaacgga aaatcaacat taaatggagt
    15901 accaaattta agagatgttc atgttatatc agatttatta agacatgtag gagcagaagt
    15961 tgaatataaa gaaaatacac ttactgttga tgcaagcaat ataaaaactt gtgaagctcc
    16021 atatgagctt gtaagaaaga tgagagcttc ttttttggtt atgggaccat tgcttgctag
    16081 atttaatagt acgaaaattt ctatgccagg aggatgtgct ataggaacaa gacctataga
    16141 tttacattta aaaggattta aagccttagg tgcaaagata gaaatggacc atggttttgt
    16201 tgaagcagca acagaaaagt tagtaggaaa taaattatat ttagatttcc catctgttgg
    16261 agcaactgaa aacataatga tggcagcttc tttagcagaa gggactacaa taatagagaa
    16321 tgcagcagaa gagccagaaa tagtagattt agcaaacttc ttgaacgaaa tgggagcaga
    16381 tgttaaaggt gcaggtacta atactataaa aataaaaggt gttaaagagt taaaaggagc
    16441 agaacataat gtaattccag acagaataga agcagcaact tatatggttg cagcagctat
    16501 gacaaaaggt gatataactg ttgaaaatgt tttaatggag catttaaaac cagttgtagc
    16561 taaattaaga gaagctggtt gtgaaataac agaaatggac aattctgtaa gagttgtagg
    16621 tccaaaagta ttaaaaccaa tagatataaa aactttacca catccaggat ttcctacaga
    16681 tgttcaagca caatttatgg ctatgcttac tgtagccaat ggaactggtg tagttataga
    16741 aactgttttt gaaaatagat ttatgcatgt tgctgaattt aatagaatgg gtgctaatat
    16801 aaaaatagat ggaagaagtg cggttgttaa tggtgtagat gagctacacg gtgcagcagt
    16861 aaacgctact gatttaagag caggggcagc ccttatttta tgcggtctta tagcagaagg
    16921 agaaactcaa ataggagaaa tataccatat ccaaagaggt tatgttgata tagataagaa
    16981 gataacagca ctaggtggtc aaatcgaaat agtagaagat taataattgg tttatagaag
    17041 aaagttataa attaataggg ttgttcatat acttaaatac aaggttttaa aagctaagga
    17101 ggagagtata tgaagaaccc attggttgtt ttgttaggat ttgtgacttg ctctgtgtta
    17161 gttccatcac ttataacttt agtttcttat aaaaatgtag aattaactga aaaaccagag
    17221 agcccagtat ctataaacaa aactattaaa aaaagtgata ttgaagacaa aggtaataaa
    17281 gaagaaaaaa atacagtgaa ctatgaaact gtaaataaaa aagcaccaat tatcaatgta
    17341 tataatcata taacagggaa aaccgaaaag atggacatgg agaactattt atgtggagta
    17401 ctagctggtg agatgtcttc agagtttgat atagaagctt taaaagctca atctgtagca
    17461 gctagaacat atgtagtata taaacaggaa catggtaaat ctagtaaaca taaaaatgca
    17521 gtagtatgta ctgattataa acattgtcaa gaatataaga gttatgatac tcttaaaaaa
    17581 ttaaatggtg aagaatggat taagaataaa tattccaaaa tccaagaagc agttagagga
    17641 actaaaggtc aaataataac ttataatgat aaggcgatac ttcctcttta tttttctaca
    17701 tcttcaggaa aaacagaaaa tagtgaagaa gtattttctg caaaatatcc ttatttaaag
    17761 tctgtggaaa gtccttatga taagtattcc cctaaatttg cgtcaacact taaaataagt
    17821 aacacagatt ttgttaaaag tcttagaagg gcatatagca ctattgtgat agatgtgaat
    17881 aatttaagta aacaagtttc aataactaaa agaagtgatg ccggaactgt agaaaaaata
    17941 aaattaggaa ataaagaatt gactggaaaa gacattagaa ctgtttttaa attgaactct
    18001 gcaaactttg acataaaatt tggagaagga tacatagatt tcgtggtaaa aggatatggt
    18061 catggtgttg gtatgagcca gtggggagca gaaggaatgg cagaagaggg ctataaatat
    18121 tatgatatat tatcacatta ctacacagat acaaaaataa aagatatata ctaataattg
    18181 tataaaatgc caaaaacacg tcaataatcc aaatgtagaa atatatttgg aggtgcaaaa
    18241 tgaagaaaaa gctgttagaa aaagatggtt tttatttatc tttgtttgta tgtgtttgtt
    18301 tattagcagt aggtggagtt tggtttacaa ataataatgt agataaattg gcttctaata
    18361 aaggaattat ggaaaatgct aataaagata gtgaagagga aatacattta attgaaaaag
    18421 acaagaaaga tgctatccct actgcaacgg attcaaaaca aaacttagaa aaagctaagt
    18481 caaaagagga aaataaatcg agtacaacaa aattaaatta cattggagat aaagtaataa
    18541 gaggatattc agaaaaagag cctagttatt ctaaaacact tgatgtttgg gaaactcata
    18601 aaggtgtaga tattagttgt actaaaggca aagaagtaaa atctctatta aacggaatag
    18661 tagtagatgt atttgatgat gaagaatatg gacaatcagt caaaataaaa agtgacaata
    18721 acattgtagt agtatattca aatctagata aaaatgttag tgttaagaaa gaacagaagg
    18781 taacagaagg acaatctcta ggtactgtag gtagtacttc tcaaatagaa agtgaagaag
    18841 gtatacatgt gcatttagag gcgtatagtg gagaaaaatc tatagaccca atgagtctaa
    18901 ttaagtaata gtataacaag tttaatcata tttggttaag cttgttatat tttttggtgt
    18961 ttttacatat atatcattaa agctattatg tgaggggggt aatctttgag atctcatata
    19021 gaggaaaggg ccatagtggt agcaaaatat atacttgaaa aaaacactac agtaagacaa
    19081 accgccaaga catttggagt aagtaaaagt acaattcata aggatgttac agaaagattg
    19141 aaggaaataa atccatccct tgccaaagaa gttaaaaatg tgctggacaa aaataaatca
    19201 gagagacata ttagaggggg attagcaacg aaattaaaat atgagaagga acataaaaaa
    19261 atgtaggtta aaaacctact ttttttattg aattaaatta taaaaagaaa tatattaagt
    19321 aattataaat agcaaaataa taatactctc aaaagattta ttttctcttg attaaagcga
    19381 gatttttgta tatgctttat tataggagta ttaaaatagg cttataatat aatctaatat
    19441 tataaataaa ggagttgaaa aaatggctgg agctgatata ggcatagatt taggtactgc
    19501 aaatgtgctt gtttatgtta gtggtaaggg aatcgtttta gaagaaccat ctgttgtggc
    19561 gatagacaaa agaacagatt ctgttttagc tgtgggagaa gaagctaaaa agatgatagg
    19621 gagaacacca ggtaatatag tcgctataag acctttaaga gatggtgtaa tttcagatta
    19681 tgatgtaact gagaagatgt taaaatcttt tatagataag atagttgata aaaagggatt
    19741 tggaagattt tttatgccaa gaattatggt atgtgtacca acaggtgtca cagaagtgga
    19801 aaaaagagca gtagaagatg ctacgagaca agctggagct agagaagtat atatcataga
    19861 agaaccaata gcagctgcca taggagctgg tgtagacata tcacagccta atggtaatat
    19921 gattatagat attggtggag gtactgtaga tatagctgta atatctctag gaggagctgt
    19981 tgtaagtgaa tctataaagg ttggtggaga tagatttgat gatgctatag ttaaatatat
    20041 gaaaaaacaa cacaatcttc ttataggaga aagaacagca gagaaaatca agtttgaaat
    20101 aggttctgca tttaagagag aagaagaaaa atatatgaag ataacaggaa gaaatttaat
    20161 tacaggactt cctagttcta taactataaa ttcaacagaa atgttggaag cacttagaga
    20221 atgtgttgag caaatagtag ttgctactca tgctgttttg gaaaaaacgc ctcctgagtt
    20281 agcagcagat ataggagatg tcggtataat aatgactgga ggaggttcat tattatatgg
    20341 attagataaa ataatagaac agaggacagg aataactgtt acaatagcag atgaaccatt
    20401 atcttgtgtt gcaaaaggta caggaatagc tttaggttca atagacttac ttgaaactgg
    20461 tgggtctttc aaaagaaaat aattaggaga gtttagaatg ttaaatatag atgaaataaa
    20521 aaaattaata ccacatagat atccattttt attagtagac aagattacag agctagaagt
    20581 tgggaaaaga gctgttggta taaagaatgt aacagttaat gagccatttt tccaaggtca
    20641 ctttccagaa tatcctttga tgccaggagt actaatagta gaagctctag cacaagtatg
    20701 tggagtggca atgatgtctg tagaggaaaa caaaggaaaa ctaggtgtat ttgcaggtat
    20761 agataaagtt agaatcaaga gagaagtaag accaggagat acattaacta tggaagttga
    20821 aatgacaact cttagaaaaa atatagctaa ggcagatgct aaggcatatg taggtgaaga
    20881 acttgtctgt aaaggtgaat taatgtttgc tttagtagaa aaataaaaac ttgaaatata
    20941 aaagtaaaaa ggtagataat ttggagtatt taatcagata ctctaaaata tctacctttt
    21001 tatataaaga attttatcat ttagtttata tttagattag gtggatacta ataaagccaa
    21061 ggctattgtt tcagctaaat ctaatataaa gctaaggcgt atattattaa atgaaagttt
    21121 attattttca tcaattttat ctacaattcc tactatagaa taatttccta tttgaggtag
    21181 ttttttacca acaccttttc cgggcaggat aggtccttct ctaatctgta tattacctat
    21241 gttactctgc gaccccaagc aagcatctat agccaagaag ttaccttgta tatgtgtatt
    21301 ttttatagta tctaaagact catatatatt taaggcgtga atagggttat ctagtgtacc
    21361 gtaaacgggg tatttaaagt tactgttctt aagtattgtt ccaactaaag ggcctaaagt
    21421 atcacctata gccctgtctg ttcctatgca aacaacaatc gtattttcat taattatagt
    21481 ttttagaact gaacttagca tctgtataac atcatcatcg ctgtagttta ctttagctaa
    21541 atacaatagg aacaccccct tcaaaaaaat atatgaataa aaagcattaa aaagacctaa
    21601 ctttaaattt cagatagata gaaaatttta tttgtcttac aaaaaaagga atagaaaaaa
    21661 tatgactaaa aatattgaca aaattttaaa aaaatagtaa gctagtaaag taaaacaaaa
    21721 cttaaatctt gattacctta tcaagagagg ctgagggata ggccctgtga agcccagcaa
    21781 ccacccaaat gggaaaaggt gctaattcct acaagacaaa agtcttgaaa gatgaggtca
    21841 gttaattagt atgtaattac gattattaac ctcttcttat aaaggagagg tttttttatt
    21901 ttgagtaaat ttctaaaatt aaaaataagg taaaacctat aataaaaatt aagaataaat
    21961 aagggggaat aaaaatggca agacatttat ttacgtcaga atcagttaca gaaggacatc
    22021 ctgataagat atgtgaccaa atatcagatt caatattaga tgcattatta gaaaaagacc
    22081 cacaatctag agtagcttgt gaaactacag ttactactgg tttagtttta gtagcaggag
    22141 aaataagtac atctgcttat gtagacattc ctaaattagt aagagaaaca gtaagagaaa
    22201 ttggatatac aagagcaaaa tatggatttg attgtgatac ttgcgcagtt ataacttcta
    22261 ttgatgaaca atctggagat atagctatgg gagttgatga aggcctagaa agtaagactg
    22321 gagaagagat agaagaagaa atagaaaaag ttggagctgg agaccaaggt ataatgtttg
    22381 gatttgcatg taatgagact ccagaattaa tgccacttcc gatatctttg gcacataaat
    22441 tatcaagaag acttacagaa gttagaaaaa caggtttagt tgattattta agaccagatg
    22501 gaaaaactca agttactgtt gaatatgaag gaagtaaagc tgtaagagta catacagttc
    22561 ttatatcagc tcaacattgt gaaacagtat caaatgataa aataagagaa gatttaatta
    22621 atcatgttat taaggaagtt ataccagcag aattacttga tgaagagact aaaatttata
    22681 taaatccaac aggaagattc gttataggag gaccacaagg agatacaggt cttacaggaa
    22741 gaaaaataat aatagatact tatggtggat attctagaca tggtggagga gctttctcag
    22801 gaaaagaccc tacaaaagtt gatagatctg ctgcttacgc agctagatat gttgcaaaaa
    22861 atattgttgc ggctggtctt gcagacaaat gtgagataga acttgcatat gctataggta
    22921 tagctagacc attatctata tttatagata cttttgggac aggcaaggtt tcagaagaaa
    22981 aacttgttga attagtaaat aaacactttg atttaagacc aggagcgata ataagagact
    23041 tagatttaag aaagcctctt tataaaaagg ttgctgctta tggtcacttt ggtagaactg
    23101 atatagattt accttgggaa agaactgaca aggttgagca gttaagaaaa gatgctttag
    23161 gtgaataatt tttaactaaa aagctgttag aatgtaattt tacgattact ttctaacagc
    23221 ttttttatta tgagttaaat atttttaata aatgttaatt tatcatcata taatgaataa
    23281 acagtattta atctggtcaa tacattttat ataatactct actaaattta tacatattat
    23341 gggatatatg ttaaaggatg gttaagaatg tgttaactaa tgttaaataa ttaaaaatat
    23401 attgaaatat cacaataatg agtctaatat aatcttaata aacttacact ttaaaaataa
    23461 ttttaattgg gggggagtag tgtatgtcag gcaaagtaaa tattaaaacg gttataagtt
    23521 ttgctggagc ttatgttgct actgtaatag gttctggatt tgcaactggt caagaaatac
    23581 tgcaattttt tacgttttat ggatatgcgg gtattatagg tggaataatc tctatggtac
    23641 tattttcatg gtttggagca gaagttatag ataagggaag agaattgaaa cttaaggagc
    23701 caataaaaat ttatcaagta tattgtggaa aatatcttgg aacatttttt gaatggtttg
    23761 gtccattgtt tttatttgga acattagtaa taatgattgc aggagcagga gctacattat
    23821 cagagtacta tggattaaat ccatatgtag gtaggattgg tatggcaatc gtttccctaa
    23881 taactgtttc attaggtttg acaaggcttt ctaaaatact aggtaatata ggacctataa
    23941 taataatatt tacactttta gtaggagcaa ttagtttatt tagcaatatt gatgctttat
    24001 caaatgctgg aaatatggtt gatagtttaa atattaaaac tgctacatca aatggatatt
    24061 tctcgggagt actttatact tgttacaatg taataatagt aataacattc ttgacaggaa
    24121 tgggagcttc tgcagtaaat aagaaagatg ctgtatgggg tggtatagta ggtggagtag
    24181 cacttatggc agcagctata atgatgaatt tggcattact ttcagacata ggaaatatat
    24241 acacaaaaga gataccagca ctttacttag ctgataagat atctccaata ataggtattt
    24301 tattctcagt agtactttta ctaggaatat acactacagc tgtaccattg ttatggtcag
    24361 taacaaaccg atttgtagaa gatgaccacc ctaagtttaa aattataaca atagtggtaa
    24421 gtatattagc ttgtatagga ggtttactgc catttgataa gttagtaggt acactatatc
    24481 catatacagg ttacatggga attttaattt tactatgtat gctttataga agaattacta
    24541 aaactgaggg gtacaaggag aataaatctg aaataagtta gtactgtaat atacttatga
    24601 aatttaattt atactctaaa tatagagtaa ttaaaataaa atagctattt aaatatggta
    24661 cctgtatttc atggtaccat attttatgga tgcagatttt ttttgtttct gataaaatgt
    24721 ataatagttg gaaattacgt taagtataaa aatataaaac ttttctacga ttatcatagt
    24781 tttaatatat aaaattatac taacatttaa atggagcgat tattagtatt tttaggagat
    24841 aaaaatggaa aaattagaag gaatgataag tgaaatagtc tttaaaaacg aagataatgg
    24901 ttatacaata gctcacctgg taaatgaaaa tgatgaaatt gtggttgtag gatgtatgcc
    24961 tactttggca attggagaaa gcatagaagt tgaaggaaag tgggtaaatc ataagattta
    25021 tgggactcaa tttgaagtga atagttttat gccagtaaca ccatcatcct tagaaggaat
    25081 ttatgtttac ttatcttctg gaatgataca tggtattggt gaaaaaatgg caaaaagaat
    25141 aattgataag tttggtgtag atacgttaga ggtgatacaa aattcaccag aaaaactaca
    25201 agaagtagaa ggcataggga gtaaaaaagt aaaacaaata gtaaaaagtt atgaagaaga
    25261 cagagaattg agaaatataa taatacagct atctcctttt ggaataacac caaattactg
    25321 cctaaaaata tataaaaaat ataaaagcag tgcaatagaa gttataaata aaaacccata
    25381 ccaacttgct gaagatataa gaggaattgg atttaaagta gctgatagta tagcaagtaa
    25441 aattggtata gataaaaact ctaaagatag aatttgtcag ggaatcttat acactttaaa
    25501 taaatcttta agtaatgggc atacatattt accagagcat gttctaatac aagattcgga
    25561 aaaattattg gagctaaatg gagaaataat aaaagagtgt gtaatgatgc tagtttataa
    25621 tcaaaaaata catatagaga aagtaaacaa tgaaaatctt atatacctta tgccatacta
    25681 tttagctgaa aatggagtat gtagtcaaat agtaaaatta tcacagtatg aatttgaaga
    25741 cttaaaaata gatatagata acgagattaa tgttttagaa gaagataaga aaattaagtt
    25801 agcagaaaaa caaattttag cagtcaaaga atctgttaat agtggagtat taattataac
    25861 tggtggacca ggaactggta aaacaactac cattaatgct atcatagata tatttgaaaa
    25921 taatggtaaa agtgtgactt tggcagctcc aacgggaaga gcagcaaaga gaatgagtga
    25981 aacttctaat aaagaagcaa aaactataca taggttgcta gaaatgggat tttcaacaga
    26041 tgatgattta acttttttta aggatgaaga agacccaata aattcagatg taataattgt
    26101 tgatgaggta tcaatggtag acattatctt aatgtacaat ttacttagag ctataaagtt
    26161 aggaacaaga gtaattttag ttggagatag tgaccaatta ccttctgtag gtgctggaaa
    26221 tgtacttaag gatatgatta attctaatat tataaatgta gttaaattaa atgaaatatt
    26281 tagacaagcg caagaaagta tgataattgt aaatgcccat aagataaaca atggagaacc
    26341 actttatcta aatacaaaag gtaaagattt cttttttata agaaaatcaa ctaatgaaga
    26401 aattttaaat gaaataatag gattggttaa tgaaagatta cctaaatttt ataaagttga
    26461 taaattaaaa gatatacaag ttttatcttc aatgagaaaa ggtgaattgg gagttactaa
    26521 tctcaatata gagttacaga agtatcttaa caaaaaagaa aaatttaaag ttgaagaaag
    26581 tttttctaaa agattattta gagttggcga taaggtaatg caagtaaaaa ataattatac
    26641 aaaaaaatgg gaaacagaag accaaaaaga aagtggagaa ggtatctata atggagatat
    26701 aggatatgtt tatcacatag ataaagataa gaaaactatt tatatattat ttgaccagac
    26761 taaaattgta tcatacctat atgatgaatt agatgaaatt gaccatagtt tttgtacaac
    26821 aatacataaa agccagggaa gtgaatttcc agttgtagta ttgccaatag cgtgggctcc
    26881 tcctatgtta cttagccgta atttattata tacagcagta acaagagcaa aaaaattagt
    26941 tgtattagtt ggagatgtga aatatcttga atatatgata aaaaataata gagttaatca
    27001 aaggtattca aatttaggat ataagctaaa taaatttaaa caagaagggt tgttgataga
    27061 ataaatatga aaatatgcaa tgataatttt attaagaatt ttaaaaatat gattaataaa
    27121 tgtttggatt ttatatatcc tgagaatata agttgcataa tttgtgataa atccataaag
    27181 aaaacaaata cttattcaat atgtaaaagt tgttttaaag aaatgaattt tatacaagat
    27241 ggttgcatga agtgtggaaa accaataata tgtcattcaa ttgaaaaaga atttattgaa
    27301 gagtgtagtt attgttttaa taaagacttt tattttgata aatcaatatc ttgtattgaa
    27361 tacaatgatg tgacaaaaaa gatggtgtta gggctgaagt ataaccaaaa aacctttatg
    27421 gctaagtata tagctcaaat aatgaaagaa aaattatatt tagaaaacat aaaatttgat
    27481 tatatattat ttgttccact tcataaaaaa aggctaaata aaagaggttt taatcaggca
    27541 caaaaaatag catttaattt aagtaaaata gtaaatattc cattattaga ttgcataagt
    27601 agaaaaaaat atacaagaat gctatataaa ttgaataaga aagaacgaaa agaagaatta
    27661 aaaaatgtat ttgttgttaa agaaaatgtt aaattaataa ataataaaaa tatactttta
    27721 atagatgata tatttactac tggtctaaca acaaacgaaa tttctaaact tttaaagtta
    27781 tcaggtgcta ataaggtttt tgtattgact ctacttacaa aagcaaatga taattatatt
    27841 atggaataat cttatatatt gacaatttta gtttaaaata ttaaaatgaa taagtgatat
    27901 ttaggtctgt ggttgaaagt ccaagccagt cgcaggcaaa gggatccacg taagttaaca
    27961 ctaaaaattg ggttaatgat catggtgcgg cttagaagta agacctaccg ataataaaat
    28021 cgagagagag tagtagtgcg gcgaacccaa gcgaaccctc cagtaggcga gtgtggggtc
    28081 aaaaaccagg tcagctaaat atcatattaa aagagagcta gcataccttt atgtatagct
    28141 cttttttata tataataaat ttaggttgag tcataaagag gtgatatgtt tgaataaaag
    28201 acaagaaaat ttattaacaa cattgctatt aaaagaagag tttataaatg ttaataagat
    28261 tgccaatgat tttgagtgtt cagaaaggac aataagaaat gattgcaaat atattgatga
    28321 atggttatct actttttgta atgcttatat caaaagaaaa cctaatattg ggattaaatt
    28381 tagtggagat ggaacagaca aaatactggt aaatcagaag ttgcgaggtg aagttaaaag
    28441 agtaaatagt gagcttcata agcaagttga gatattaaat ttactacttt gtaataataa
    28501 aaaagttact ttaaatgatt tatctaataa gttgtacata aataaaaata ttgtcagaga
    28561 agaaataagt aaattatcag tagcattaaa taattataat ttaaatatct cgactaaaaa
    28621 aggcagtggt atatatattg aaggggatga gaaagacata agaataatgt tagtatcatt
    28681 cttatttaaa tatatagata aatacagatt aaacatagat gaaatagagt acttccatat
    28741 agtagatata ataattgtaa aaaacataat gcttactata gaggaggata tggatataag
    28801 acttacagat atatcattca agcaattgat gctatttttg cttataaata taatacgaat
    28861 aaggctggga aattctttaa aagtatcaaa tactaagaaa gagcttaagc taaaaatgct
    28921 tatattggat atagaagatg aattgaaatc taatttatcc atagtattaa attatgaaga
    28981 aaaattgttt atagaatctt taatattggg tatgaacaaa caaataaata gtaataatat
    29041 taagaaatct ttatatctaa atgaagaatt agcaagttat acaaaaaaaa ttataaattt
    29101 agtatcagaa gaatcaggaa taaattttaa taatgatata ttactttatg aacaattaat
    29161 atgtcatttg aatgttacaa tgcatcaaat gaaaagcaat gtttacttag aaaatccact
    29221 tttagataac ataaaaacta aattttgttt tttatttaca gtaatatcaa gttcaataga
    29281 aattgaatta aaaggaaaac aaataacgga agatgaaatt ggttatctta cacttcactt
    29341 tcaaacctct ctagaaagaa aatacagcaa aagggaatca aataaaaagg caagtattgt
    29401 atgtccattt agttttggag taagtatgct attaaaagta aagatagaaa agagatttga
    29461 taacataaaa attattgaaa cattaagaga ggaagattta aaaagagata actttaataa
    29521 gactatagat tttataattg catttcaaga gtatgaaaat atccaaaaac caatttttat
    29581 aacaacacca ttatttactg atgaggatga gaataaactt aaagagttca caaacaaaat
    29641 aaaagaaaaa gacacttcat ataaacttat gaacagatta atgatgtctg attatttaat
    29701 aagagagtta gaacatgatg atatttatga agctataaca tatttaacta atttattaat
    29761 tgaaaaacat tatgcagaaa aagagtattt acaatctatt ataacaagag aaaagtcata
    29821 tccaaccaat gttggaaaag gaatattatt ccctcatgga gacatgaaat atataaaaca
    29881 atctgtttta tgttttgcaa ggttaaaaac accaataaaa attagaaatg gaaaggatat
    29941 aaagattata cttttgttag catataaaaa agatgatgat agaaaagtat ttagagaatt
    30001 atttaaagaa atatctaatt taacagagga tgaaaatatg ctagatattc taagtaaatg
    30061 tgacattgaa aaggtaaaag atattcttat ataaaattca tagtgaacta tggataagca
    30121 caaaattttc catgtgatgg aaaaagttgt gcatgttttt ttgtttttat aagatattat
    30181 atgtttgtaa ggtataaata atctaaaagt agagaagcaa cttaggagga aaaaatggaa
    30241 gaaatagttt taaagattat aattcatgct ggaaatgcaa aatctatgct ttatgaggca
    30301 cttgactatg caaaggaaaa cgattttaaa aaagcagatg aattaattga aaatgcaaat
    30361 gaagaaattt taaaagcaca taaagtacaa actgaactta tacaaaagga agctggggga
    30421 gataaatcag atatatctat tttattgatt cactctcaag accatttaat gacttgtatg
    30481 agtgagagaa accttataaa agaaatgatt atgttaagaa aagaaattca aaaactaaaa
    30541 taaaaaatta agggggattt aaaaaatgaa aagaaaagta tatttatttt gtagctttgg
    30601 tatgtcaaca agtttacttg cagataaaat gcagaaggta gcagatgaac acaatttacc
    30661 aatagaagta gaggcatttc cgatagcaga aattgacaaa atagttgaag aaaaaaatcc
    30721 agattgtata ttattaggac cacaagttaa atacatgcta aaggagttaa aaccaaagtt
    30781 tgaagctcaa ggaaaactta tagatataat caatgaagtt gattatggaa ctatgaatgg
    30841 ggaaaaagta ttaaaacttg ctataaagct aataaaatct aaacaagcgt agaattatag
    30901 taaaaaataa taggaaatga gggtggatag atgaatagat tggaaagaat acttatgcca
    30961 ttggctgaaa aaataggtaa aaataaatta ttaattgcaa ttagagatgg ttttttagta
    31021 tcatcaccat tattaataat aggttcatta tttttattag tagcaaattt tcctataaaa
    31081 ggatggaatg aatttgtagg tcaaattttt ggtccagatt gggctgtaaa attgcaacaa
    31141 ccaactgtag caacatttga agtaatgact ctacttgcag ttttagggat tggatattct
    31201 tatgcaaagc aaatagatgt agacccaata gcatcagcag cagttgcaat agttgccttt
    31261 tttataataa caccatttgt aataccatat acaccagaag gaacagaatc agtttacatg
    31321 gtaacaggta taccactagg ttggatgggt tctaagggaa tgtttgtagg tatgataaca
    31381 gcaataacct ctgtaaaatt atttgctgca gttgtaaaaa aaggatggac attgaaaatg
    31441 ccagatggag ttcctccaac tgtatcaaaa tcatttgcag cattaatacc aagtgcaata
    31501 gttatggtag ttttcttttt agttaagata gcttttgaag ctacaccata tggaagtata
    31561 catgaattta tatttaagtt tttacaaatg ccattattaa aattaggaaa tagtttaggt
    31621 gctatggcaa tcgcttatat attcttacat ttattctggt tctttggaat aaatggaagt
    31681 agtgttgttg gggctgtgta taatccaata ttaaaaattc tatcagctga aaacttagct
    31741 gcttttcaag ctggttcaaa aataccaaac ataataacag gtcaattcca agatatgttt
    31801 gcgacatttg gtggagcagg tagtacacta tcattagtca tagctatgtt attaatatgt
    31861 aaaagtaaac gtataaagtc aatgggaaaa ctatcaattt taccaggaat atttggtata
    31921 aatgagccat tgatatttgg tctaccaata atgcttaacc caatactatt gataccattt
    31981 gcaatagttc caacaataaa tataataata gcttacttct gtatgtcagc aggattagtg
    32041 cctcttacaa atggagttca gttaccgtgg acaactccaa ttatattctc tggtttttta
    32101 acaacaggat ggcaaggtgc agtcttacaa ttagtacttc ttatactagg aatatttatg
    32161 tatataccat ttataaaaat gctagataag caatacttga gagaagaaaa tgaaactgtt
    32221 gaagaggaag atgatgatat atcttttgat gatttagtat tataggagga agattatgaa
    32281 gaaaatagtt atgatattgg accaaataca agctggagct ggaggaaaag agaaaagtaa
    32341 tatacctcct gctggaaaat caagcccatt aggaccaggt gtaatgatgg agcaattttt
    32401 aaatgaatct aaggtaatag ctaccttatt ttgtggggat gaattttttg taaataatca
    32461 agaagaagta acgagtaaga tgatagctat ggtaaaaaaa ttaaaccctg atgtagtaat
    32521 ctgtggacca agctttaatt acgaaaactt ttctaagatg tcagcaatac tgtctaaaaa
    32581 tataaatgat aaaacagata taccagcttt tgcagctatg tcagaagaaa atatagatgt
    32641 gataaacgaa tataaaaatg acatatgtat agttaagact ccaaaaaaag gtggaattgg
    32701 tcttaatgat tcattaaata acatatgtaa attggcaaaa gcaatagcaa ataaagaaga
    32761 tataactttg atgaaagaag agttttgtta tcaataagag tattaattat tagcaagttt
    32821 aaatattata ctatttataa taagcataca ttagtaaaat ctatataatt taaggagggg
    32881 aattttaaca tgtggggaat gatagcgaca tggagaatgg ccctagaagg aataactgag
    32941 gcatcagaac atttagaaaa aggcatggaa gcaggtgatg ctgtagagct tgctgtaaga
    33001 agagttgaag actatccatt ctataaatcg gttggatatg gtggacttcc aaatgaaaat
    33061 tgtgaggtag aacttgacgc tgcatttatg gatggtaaca cattagatat tggcgcagtt
    33121 gctggtataa gagattattc taatcctgtc agcatagcta ggaagttaag ttatgagaag
    33181 gtaaataatt ttttagttgg aataggtgct gaagattatg ctcataaaaa tggatttgaa
    33241 agaaaaaata tgttgactga tagagcgaag ttacattata aaaagagaaa aaaagagact
    33301 ttagacaaag gattaagccc atatgctggt catgatactg ttggtatgat atctttagac
    33361 aaaactggaa aaatgtgtgc agctacatca acaagtggat tgtttatgaa gaaaagaggt
    33421 agaataggag attctgcact atctggttca ggattctatg ttgatagtag tataggagga
    33481 gctacagcta ctggtttggg agaagattta atgaagggat gtatatctta tgagatagtt
    33541 agacttatga aagaagggaa gcatccacaa gaagcatgtg atattgcagt aaatgaactt
    33601 gataaacagc ttatcgaaag aagaggaaaa gctggtgatt tatcattagt tgccatgaat
    33661 aataaaggtg aatggggagt agctacaaat atagctgatt ttactttttc agtagtaact
    33721 aaagaagaat ctcctactgt atatatttca aatagagtag atggaaagac tatacataag
    33781 gttatatcaa atgagtggat ggaagcatat gaggctagaa taaaagcacc aatagaagta
    33841 agtgagtaaa gtagtggtta cataattaaa atagagtttt ataataagga gatttttatg
    33901 aatatcataa gtaataaaat agatgagtta aaagaagatt tattaagtga tattatagat
    33961 attgtaaaaa tacctagtgt caaaggggag tcagaaaatg gcttcccttt tggcgaaaaa
    34021 gtaggggagg cacttaataa agcattagaa atatcagaaa aattgggatt taaggttaga
    34081 aacttggata attatattgg ttatgcagag catggagata gtgatgatta tgtttgtgta
    34141 attggacatg tagatgttgt acatgaagga gatggttgga agcatcagcc ttataaggga
    34201 gaagaaacta atggaagaat ttatggacga ggagtactag acaataaagg acctataatg
    34261 tctgccttat atggattata tgctattaaa gaattaaact taaaattaga taagagagtt
    34321 agaataatat ttggtacaaa tgaagaaagt ggatttgaag atataccata ttatttagaa
    34381 aaagaaaaag cacctattat gggatttaca ccagattgta aatatcctgt tgtatatggt
    34441 gaaaaaggca tggcaaagat tagaataaaa agtaaaatca attatgaaga agatgtatac
    34501 ctaggtttta tagaaaatat gtctgaaaat gtgcttgtta catataaaga gctgaatata
    34561 gaaaatagtg atactatatt agatataaag gtaaaatacg attttagtta taaattgaaa
    34621 gatgtattgg atgaaataaa agctagtttt ccaaatagta tcgatataga ggtaatttct
    34681 aattttaatc ctgtatattt tgataaagag agtaatcttg ttaaaaagct tcagctagct
    34741 tatgaaaggg ttacatcatt agatggaact cccgtaacaa ctaatggagg tacgtatgca
    34801 aaagtaatgc caaatatagt tccgtttgga ccttcatttc caggacaaaa aggaatagct
    34861 cataatcctg atgaatacat ggatatagaa gatataatat taaatgcaaa gatatttgca
    34921 aatgcaatat atgaattagc aaaggagtag ttatgttaga aataatagga atgtcagtag
    34981 aagacgctaa aattatagaa gattgtggtg cagatagaat agagttagtt agtgctttaa
    35041 ctgaaggtgg attgacacca agttttggtc ttatagaaag tgttgttaac agtgtaaaga
    35101 tacctgtgaa cgtaatgata agacatcatg cgaaaagttt tgtatacagt aaagaagata
    35161 taagtataat gcaaaaagat ataggtgtgg ttaaagagat tggtgctaat ggagttgtat
    35221 ttggtgtatt ggataaaaac aataatatag atgagaaaaa tctaaatgtt ttattgaaat
    35281 gttgtgataa tcttgatgtt acatttcata gagccataga tgaatcaaat acaatagata
    35341 gtgttaaaat tttgaaggat tatgataaaa ttacgaatat attaacatca ggagggaaag
    35401 gaagtatagt tgataacata caaatgataa agaatatgat gcttagttct aatcacataa
    35461 aaattctttt aggtggaggt cttaatttta ataatataga aaagataaag gaattgacta
    35521 aggcaagtaa ttttcatttt ggtacagcga ttagaataaa taacagtcct tttgaagata
    35581 tagatagaca aaagcttaaa caactcgtaa atataatttc aagatagtat atgaatataa
    35641 aatacttagt ttaaataaag agagtaagtt aattataatt tgggtatatg aatgttaata
    35701 ggtattaaat atgaaattat tgttgagtat gctttataac ttttaagtag ttttaaatat
    35761 aaataatgaa agaaaagata gattatggag gttttagagg gtttatattt attaaaaaat
    35821 tataaaggta tattggcata taatacacat aatttcctaa aattaccatc ttatgacatg
    35881 aattcacata ttaataaaca attaatggag atttttgtgg atagaaatag gagtaaaaat
    35941 agctttaaat aaacaaaaac aagagttatt aacagtttta tccacattat ccacaggtgt
    36001 ttgtaataat attaatccac ataagtatca atgtaactaa aaatgataaa taattctatc
    36061 aattgaatag aaaaaatcat aaaagtaaat aataaaaaga ggattatgag agttcgtgta
    36121 gaatatatta ttaaagtata aattttctta tactggcata attgaaggga gatgtattta
    36181 tgaacataat tatatctgga aaacaaatcg aattaactga tgcaataaaa aataaaatcg
    36241 aaactaaact aagtaagtta gataattaca ttcatccaga cactgatgta aaagttacag
    36301 taagtgcgag aaaagcaaga caaaaaatag aagtaacaat agctactgta aatggtccta
    36361 taattagagc agaagatatg caggaaaatt tatacacagc tatagatgtt gtatatgata
    36421 aattaagtaa acaattaaaa aaatataaaa agagattaca agataaacat aaagacaaca
    36481 agagtataag attccaagat gcagagataa atctagctga tgaacaagag gattatgggg
    36541 atgaaaatca agaattagta attcaaagac gtaagaaatt tagtgttaag ccaatgagtg
    36601 aagaagaagc tgtattacag atggaattaa taggacatga tttttatatg ttcaagaaca
    36661 tagattcaga tgaaatagca gtagtttata agcgtaataa tggtggatat ggtataatag
    36721 aacatgaata aagcaattta gatttaatca tattagctat cgataaataa attatcgata
    36781 gctttttaaa ttaatattaa aagtatagtt tttatggtaa aatgaataat agtacaataa
    36841 agaacgagga gagatgtaaa atatgagctt tatggataat ttattcaaca tggcagataa
    36901 aaaagaatta aaaaagttca ataaaactgt tgatataata gattcattag aacctaaatt
    36961 tgaatctatg gcagactcgg aacttaaaaa tatgacaaat atatttaaag aaagattagc
    37021 aaatggtgaa agtatagatg atatacttcc agaagctttt gcagtagtta gagaagtttc
    37081 taagagagtg ttaggactta gacactacag agttcaaatg attggaggta tagtactaca
    37141 tcaaggaaga atagcagaaa tgaaaacagg tgaaggtaaa actttggttg caactgctcc
    37201 tgtttatttg aatgcgctta ctggaaaagg tgtacatgtt gtaacagtaa atgattacct
    37261 tgcaaaacgt gatagagacc aaatggcaaa aatctatgag tttttaggaa tgagtgtagg
    37321 cgttattata cacggtcaaa atccaaaagt tagaaaagaa caatatgatt gtgatataac
    37381 ttatggtaca aataatgaat atggatttga ctatctaaag gacaatatgg taatacacaa
    37441 agagcaaaga gttcaaagag gattaaatta tgctatagtg gatgaggttg actctatact
    37501 aatagatgag gctagaacac cacttatcat atctggacct ggagacaaat caactcactt
    37561 atattcagat gcaaacacat ttgttttaac cttaaaacca gatgattatg aattagaaga
    37621 aaaagataag gctgtatctc taacagcatc gggtatacaa aaagcagaag tgtattttaa
    37681 tgttgataat ataacagata tatctcatac agaattatac catcatataa atcaagcttt
    37741 aagagcacat gtaataatga agaaagatgt tgattatgtt gctaaagatg gagaaatagt
    37801 aatagtagat gagttcacag gtagacttat gtttggtaga agatattcag aaggtttaca
    37861 tcaagcaata gaggctaaag aaggtcttaa aatacaaaga gaatctaaaa cactagctac
    37921 tgtaacattc caaaattact ttagaatgta caaaaaactt tctggtatga ctggtacagc
    37981 aaaaacagaa gaagaagagt ttaaagcaat ctacaaaatg gatgttttcc aagtacctac
    38041 taataagctt atgataaggg aagacttacc agactgtgta tacaaaagtg aaataggtaa
    38101 atttaatgct gtagcacaag aaataattga aaggcacaaa gtcaatcaac caatacttgt
    38161 aggtacagta tcaatagaaa aatcagaatt attatcacaa atactaaaga aaaagggaat
    38221 aaagcacgag gtattaaatg ctaagcatca tgataaagag gcagaaataa tagcacaagc
    38281 tggtagactt ggagctgtta caatagctac taatatggct ggtcgtggta cagatatagt
    38341 tctaggtgga aatccagact ttttaactaa gagagaaatg agaagaaatg gatttaaaga
    38401 agaaatagta aatagagtag atactccaat agaaggtata cctgtaaaag gtaatgaaat
    38461 tctatttgaa gctagagaag agtatgaaaa actatttgaa aagtttaagc aacaaacaca
    38521 agaagaacaa aaacaagttg tagaagcagg tggacttgct ataataggta cagaaagaca
    38581 tgaatctcga agaatagata accagttgag aggtcgtgct ggacgtcaag gtgacccagg
    38641 gagttcaaga ttctatatag gtcttgatga tgaccttatg agattgtttg gtagtgatag
    38701 aatatctggt attgtagata aaataggtct tgaagaagac atgccgatag aacatagaat
    38761 attatctaag tctattgaag gagcacaaaa gaaagtcgaa ggtaaaaact ttggaataag
    38821 aaagcatgta cttcaatatg atgatgttat gaataaacaa agagaaataa tatatgcaga
    38881 gagaaaacgt gtattagaag gtgaagattt acaagaacaa atacaatcaa tgactcattc
    38941 tataatagaa gaagcagtta ctctttatac acaagataaa ggatttgatg aagaaggatt
    39001 caaagaacat atgtataatt tgttcttgcc aaaaggtagc atagaaatac cagaaataga
    39061 aaaacttaat ccagttgaaa taacagaaaa agtttatgaa atagctatga agatatatac
    39121 ttcaaaagaa gaacaagttg gctatgagag aatgagagaa gttgagagag taatcttact
    39181 tcaagctgtt gataaccatt ggatagacca tatagatgct atggaccaat tacgccaagg
    39241 tattggtctt cgtgctgtag gtcagcaaga cccagttatt gcttataaaa tggaaggttt
    39301 tgatatgttt gatgaaatga ataaacatat caaagaagat actgtaagat atttatttaa
    39361 tataacaata gaaactccag tggaaagaaa agcagttgta gatgttgaaa atctatcttc
    39421 tccatcagat ggaacacttc caacaagtaa aactgttaaa aaagatgaaa aggttggaag
    39481 aaatgactta tgcccttgtg gaagtggtaa aaaatataaa aactgttgtg gaagataatc
    39541 tcggaggatt aaatgttaaa gttacaagag tatagacata gtatggagca gatggcttca
    39601 aatttagaag aattgagggt ttctctttga catagcttca ttgatgaaaa atatacaaat
    39661 taatgaagaa aagatgaatc aacaagattt ttggaatgat aatgaagtcg ctcaaagagt
    39721 acttcaagaa aataaatctt taaaagagac attagaagaa tatgaaagct taaaaagctt
    39781 attagaagat atagaagtat taattgaaat aggtttagaa gaagataatg attctgtaga
    39841 aagagatata gaaaagtcca tagaatcaat ggaagaaaaa ttatctgaaa tgaaaataaa
    39901 gactcttcta aatggagagt atgataaaaa taatgctata ttatctataa atgctggtac
    39961 tggtggctta gatgctcaag attgggctca aatgcttttg agaatgtata taagatggtc
    40021 agaaagtaag ggttataaag ttaagctttt agatataata tcagaccctg aagcagggat
    40081 aaagacagct acaattcttg tagaaggaac aaatgcttat gggtatttaa agagtgaaaa
    40141 aggagttcat agattagtga gaatatctcc atttgaccca tcaggaaaaa gacatacttc
    40201 atttgcatct atagatgtaa caccagagtt agatgaaaat atagaagtag aaattaatcc
    40261 atcagattta aagatagaca catatagagc ttctggagct ggagggcaac atgtaaatac
    40321 gactgattca gcagttagaa taacacatat ccctactggt gtagtagttc agtgccaaaa
    40381 tgaaaggtct cagcatctaa ataaggacag agctatgaga cttttaatgg cgaaattgat
    40441 agaattaaaa gagttagagc aaaaggaaaa aatagaagat atacaaggca aatactcaca
    40501 aataacttgg ggtagtcaaa ttaggtcata tgtttttcaa ccatataaat tggttaaaga
    40561 ccatagaaca aatgctgagt ttggaaatgt agatagtgta atgaatggaa atatagactt
    40621 gtttattaat gaatacttaa aaatgaataa aatagtttaa taaatatgca ctgtaattaa
    40681 gatatatgta taagaaggtt agtgcttaat atgcacttac agcacttagg ctgaggacga
    40741 taaccctcag ccttcgttct gttttgttat acttatgaaa aactaacatt aggagtgaat
    40801 aaattggata taaatcaaat cttaaaaaaa gagtttaatc ttagagatga acaaataaat
    40861 aatacattaa aacttattga tgaaggaaat acaataccat ttatagctag atatagaaaa
    40921 gaaatgactg gcgaaatgag tgatgttaca ctaagagaat tttatgaaaa attaatgtat
    40981 ttgagaaatc ttcaaagtag aaaagatgat gtagttagac taatagatga acaagggaaa
    41041 ttaacagatg aaataacaca aaatatagaa aaagcaaaaa cattacaaga agtagaagat
    41101 atatatgctc catataaaca aaagaaaaga actagagcaa caatagcaaa agaaaaagga
    41161 ttagaaaatt tagcattgtc aatactagag aataatttag ataatataga aatagaggca
    41221 aagaattatc ttgatgaaga gaaagaagtt ctttctattg aagatgctct aaaaggtgct
    41281 agagatataa ttgcagaact agtctcagat gatgctaaga ttagaaagta tataagagaa
    41341 ttagctttaa gagaaggtat gatagttagc aaaagtgcta ctgatgagaa atcggtttat
    41401 gatatgtatt atgattatag cgaagcagta aaaagtatgg ctccacatag agttttggca
    41461 ataaacagag gagaaaaaga aagcttccta aaggttaagt tggaaataaa taatgataaa
    41521 gttttaaatt acataataaa cgaatatgta aatgataaaa atttcaaaaa taaagaagaa
    41581 atcgtaagtt caatagaaga ttcttataaa agattaattt tcccatcaat agaaagagaa
    41641 ataagaaatc atttaacaga aatagctcaa gaaagagcta taagtgtatt tggaaagaat
    41701 gttaaaagtt tattgcttca accaccagtt aaagacaaag tagttatggg atttgaccct
    41761 gcatttagaa ctggatgtaa aatagctgta gtagataaaa atggtaaact actagattat
    41821 acaacagtat atccaacaga ccctcaaaat gatgtagagg gagcaaagaa agttttaaaa
    41881 ggattgattg aaaaatacga tatagatata atttcaattg gaaatggtac tgcatctaga
    41941 gaatcagaaa cttttgtatc agaaatgata aaagaaatag atagcgaagt gcagtatgtg
    42001 atagttagtg aagcaggagc atctgtttat tcagcatctg aacttgcaaa tgaggaacat
    42061 ccagatataa atgtatcaat aagaggagct atatctatag ctagaagatt acaagaccca
    42121 ttagcagagc ttgtaaaaat agaccctaaa agtattggtg ttggtcaata tcagcatgat
    42181 ttaaacaaaa agagattaga agaagtacta gatggagttg ttgaagattc tgtaaatagt
    42241 gttggagtag atttaaatac tgcatcatac tccctactag aacatgttgc aggaataagt
    42301 aaagctatag caaaaaatat aatagcatat agagaagaaa atggagattt tacgtcaaga
    42361 gcacaattga aaaaagtaaa aagactagga cctcaagcat ttactcaatg tgctggattt
    42421 atgagaatct tagagggtaa aaatccacta gataatacag gtgtgcatcc tgaaagttat
    42481 gatatatgta aaaaaatgat tgagataatt ggatattctt tagatgatgt aaagaataag
    42541 aatataggtg aaatagatga aaaaataaaa gaaattggct tgagggaatt aagtgaaaaa
    42601 ctggaagtag gacaagttac gttaaaagat atcatagctg aaatcaaaaa accaggaaga
    42661 gacccaagag aagaaggtat aaaacctata cttagaactg atgtcttaaa aattgaagat
    42721 atacaagagg gtatgacttt aaaaggtaca ataagaaatg ttgtagactt tggtgcattt
    42781 gtagatattg gaataaaaaa tgacggatta gttcataaat cagaaatgag taatagtttt
    42841 gtgaaagacc ctatgagtat tgttacagta ggtgatatag tagatgtaaa agtcattgga
    42901 attgacttaa acaaaaaaag agttgcctta tcaatgaaaa aatagataag tttatgtatg
    42961 gttatagtac ttagattcta tatttgtaaa taagattact cgagtgtata aatgttaaaa
    43021 atttgtaaaa gtatatagta agaaaatata ttaaaataag aacgatagtc ttgaaaatca
    43081 ggaggaaaat gaatgatttt tataaaaaat ggtaaaataa ataccataac aaatgggata
    43141 attcatggag atatacttat agatgaagga aaaataatag aaattggaga agatttgata
    43201 gctcctttag atgtagaggt aatagatgct agtaataaac ttgtgtttcc tggatttata
    43261 gatgctcata ctcacttagg tttatgggaa gatggtatag ggtttgaagg agcagatgga
    43321 aatgaagaga ctgacccaat aacaccacaa ttaaatccta tagatggaat taatccaatg
    43381 gatagaactt ttaaagaagc ttttgaaggt ggaataactt ctgtttgtac aacccctgga
    43441 agtgcaaatg ttatgggtgg tcaatgtata gctataaaga catgtggaaa aagaattgat
    43501 aaaatggtaa taaaaaatcc agttgcatct aaaattgctt ttggagaaaa tccaaagagc
    43561 tgttatggtc aggatgataa atctcctcaa actagaatgg ctatagctgc tttacttaga
    43621 gaaaacctaa aaaaagcaga agaatattta gaggatattg atatgtatga gagtcatgat
    43681 gatgaagact gtgaaaaacc tgaatatgat ataaaaatgg aaagtttaat accagtttta
    43741 agaagagaaa ttccttttaa ggcacatgct catagagcag atgatatgtt tacagcaata
    43801 agaattgcaa aagaatttaa tttaaagctt acattagacc attgtacaga aggtcatctt
    43861 attgtagatg aattagtgga agaagggttt cctgtaatag tagggccatc tttgtcagaa
    43921 aggtctaaat ttgagcttag aaatttgaca tttaatacag caggaatact ttcaaatgca
    43981 ggtcttgatg tatgtataat gacagaccat ccagtaatac cagttcaata tcttccaata
    44041 tgtgcaggta ttgcagtaaa acatggtatg aaagaagaaa aagcaataga atcaattacg
    44101 ataaatccag ctaaggcttt aggaatagaa gatagagtag gctctataga agttggtaaa
    44161 gatgcagacc tagtaatttg ggataattct ccactagaga ttcaaagtaa tgtactttac
    44221 actataataa atggcaaagt agtgtatgag aaaaaataac ttgacaatta ttaaaaaatt
    44281 aactaatata agagtgtaag gaaaattaaa aaatcaaaga agatcttcgg ggcagggtga
    44341 aattccctac cggcggtata gcccgcgaac tacttaggtg gttgatttgg tgagattcca
    44401 aagccgacag taaagtctgg atgagagaag atatttatgg aagataaata agtttatttt
    44461 agtttgttta aactatatcc ataaaaactc gaagataggg tcttcgagtt ttttgttttt
    44521 cctaaaaaat ataaacatat tgattagggg gaaaataatt atgcaaaaca cagcaaaaag
    44581 aggcaatatg atttcaacaa gaactttagt agtaatgtca attttatctg caatatctta
    44641 tttattaatg tttattcaag caccaatacc aggtatattt ccagatttct taaaaattga
    44701 cttatcagat attccagcaa tattcggagg aatgtctcta ggaccattag ttggatttgg
    44761 aatagttgta gtaaaaaatg tgttacaagc aataacagca acaacaactg gaggaatagg
    44821 agaatttgca aatatattaa ttggtggttc ttatgtaatg atactttgtt attcatataa
    44881 gagaagtgga gatttaaaag gtgtactgat tggttttgta ttaggaacaa tatctatgac
    44941 tataattggt tcattagtaa attattttgt aatgttgcca ctttatggtc aacttatggg
    45001 actagatgct ataataggtt taggttcagc tataaatcct caagttcatg atttattcac
    45061 ttttgtaatt tggatgatag ctccatttaa tgtgctgaag gctgttataa tatctttagt
    45121 aacattgcca ctttataaaa aaatgggaaa tataataaag aaataattag acaagaaaat
    45181 ataaaattaa atgtattgaa tagccataat ttatttataa cttcagtcct atattgtgat
    45241 tgatatatta aatatattat ggctttatta atttatcaaa aaatgtatat aaaataagaa
    45301 atatttagac aaatttaaat tttaaaataa aggaagaatt aaaacgatta actattaaaa
    45361 tatgaaaaag gtatctacaa tatacagagc gaatttataa taaacaaaaa ataaagttat
    45421 tattaaaaaa ctataaagat taataatcaa acttttaata ataactaatt taataatgat
    45481 aggggtgtat attcatgcat ttatctaaaa aaataaaaaa attattttta gatgataaag
    45541 ggtattattt taatagtaat tggaaagata ttcatattgg tatttatgat acaaaattag
    45601 gagattcttt aaagtatatc actaatgaat atattgaaaa tatggaaagg gtaaaatacg
    45661 attatcgtga attaaaaaat atagaaaatc aaactgatgg tatgtgtatt ttagcaataa
    45721 ggcagaatta caaggcatta gagtatgtaa aaaatcaaac cgaagaatta tgtttagaag
    45781 caataaagta taattataaa gcacttgaat atgtaaaaga acaaacagaa tacttgtgtc
    45841 ttgaagctat aaaaaaagat tgtaatgcac ttaagtatgt acgaaataaa acagaagggc
    45901 ttattataaa agcaataagt cattcttcca atatagatgt tgtaagtata ctaaaggctt
    45961 tagagactca aacaagaagg atatgtttag aagcaattaa aaaagatggc aggtgtcttg
    46021 catacgttag agaacagagt gaagaattat gtatagaggc cattaagcaa aattataaag
    46081 cattaaaata tgtaaaaaat caaacggaaa aaatgtgtat agaatccgta agacaaaatg
    46141 gtatggcatt acaatatgta aataaacaaa ctgataaaat atgtatagaa gctgtcaaac
    46201 aagatggaag aagtctacaa tttgtcaaca ataagacaga agaaatatgt ataaatgcta
    46261 taagatattt aaataaaaaa tataatatta aagatgtact aagttacata gataagtata
    46321 cagaagatat atgtatagag attgtaagac aaaatggaaa aatgcttatg tatataaaaa
    46381 atcaaacaga aaaaatgtgt attgaagcag taaaagagaa ctataagtct cttaagtatg
    46441 taaaagagca atctgagaga atatgtaaag aagcattgaa gcaaaaccat aaagcaaaag
    46501 agtatgtaaa aattgctatt gatgattgta tataaggtgt agatacagcc atcaatagtg
    46561 aaagataaaa aaagataaag acaagtactt tataataatc taggcttata ctataattaa
    46621 ttaagcagtg atataaattc acatgacgat tatatcaaaa tggagatgaa ttgatggcaa
    46681 aaatatattt agaaaatgaa aataaaacta gagaaatagg atataaatta ggtaaacttt
    46741 taaaagaagg cagtgttatc tgtttagtag gagatttagg agctggtaaa actacaatga
    46801 cacaaagctt ggcagattca ttagggatag aagattatat aacaagtcct acttttacta
    46861 taataaatga atatgaagga aaaatacctt tgtatcattt tgatgtttat agaataggta
    46921 gtagtgatga aatgtatgat attggttatg atgaatatgt aaattcaaat ggaatctgta
    46981 taatagaatg ggctaatcta atagaagata tacttccaaa agaatatttg aatatagaat
    47041 tgagatataa agatgaagga agagaaatga tattaactcc aaaaggagag ttttataaag
    47101 agatagttga ggagttgatt aaataatgaa aatactaggt atggatacgt cttctatggc
    47161 agcaagtgta gcagttgttg aagatgataa cttaatatgt gagtttacag taaacaacaa
    47221 aaaaactcat tcacaaaagc ttatgccaat gatagaaaat atgttgagta tgagtgattt
    47281 aagtataaaa gatatggatt tattagctgt atgtataggt ccaggttcgt ttacaggact
    47341 tagaataggt atggctacag taaaagctat ggcccatgta aataatatac caataatagc
    47401 agtaaattct ttggaatctc tagcaaataa cataaatttc tgtgatagaa agatatgttg
    47461 tatattagat gcgcagaaaa atcaagttta ttcagctaaa tacaagtttg aaaatggaga
    47521 gatgatagaa ttagatggtg ttgatgttgt agagtttgat gctttagtag atgaaattgt
    47581 atcaactaat gaagaattta taatagttgg agaagctgta tacaaatata aagacaagtt
    47641 agagaatata aaaaatataa aaataccttc accagctaat aatgtatcta acgcaggaag
    47701 cttatgctct ttagcattaa ataaatataa taaaaatata gatgtacata cttgttatac
    47761 gataaatcct atgtatatca gaaaatctca agctgaagta caatatgatg aaaaaatgaa
    47821 gaggctaaat aatggaagat aatacaaaac taatagtaga tgatataaaa atagaagaaa
    47881 tgactacaga agatatagat gaagtttttg aagtagaaaa aaattgtttt gaagattatt
    47941 ggtcaaaaga gtcttttaga aaagagttgt ctaatgaagt agcaaggtat atagtagcta
    48001 agttaaatgg aaaaattgtc ggatatgtgg gaatttggct tatacttgat gaagggcata
    48061 taaataatgt ggctgttcat agtgactata gagggaaaaa aataggggac aagttgataa
    48121 aaggcatagt tgatttatgt aaagataata atattgcatc tatgacatta gaagttagag
    48181 catcaaataa aatagcacaa aatttatata gaaaatatgg atttaaaatg ggtggaataa
    48241 gaaaagaata ctacagtgat aataaagaag atgcaattat aatgtggaac caattaaaag
    48301 aggtgtagag tttatgagtg atataataac attagctata gagagtagtt gtgacgaaac
    48361 agctgcatca gttttaaaaa atggtagaga agtactttca aacataatct ctactcaaat
    48421 cgagacacat aaaaaatttg gtggtgtagt tccagaagtg gcctcaagaa aacatgtgga
    48481 aaatattgat atagttgttc aagaagcttt agacaaagca aatataggtt ttaatgatat
    48541 agaccatatc gcagttacat atggacctgg gctagtaggt gctttattgg ttggtctatc
    48601 ttatgctaaa gcacttgcat atactttgaa tattccactt gtaggagtaa atcatataga
    48661 agggcatcta agtgcaaact atatagagca taaggattta aaacctcctt ttataacatt
    48721 aatagtatct ggaggtcata ctcacttggt tgaagtaaaa gattatggca agtatgaaat
    48781 tttaggaaaa acaagagatg atgcatctgg agaagcattt gataagattt caagagctat
    48841 gaatttaggc tatccaggag gtcctattat tgataatctg gccaagaatg gaaataaaca
    48901 tgctatagaa tttccaagag cctatttaga agaagatagc tatgatttta gttttagtgg
    48961 attaaaatcc tctgtattaa attatctaaa tggaaaaaga atgaaaaatg aagaaatagt
    49021 agttgaagat gtagcagcat ctttccaaga ggctgtagta gaggttttat ctactaaagc
    49081 actaaaagca gtaaaagaca aaggatacaa tatcataact ctttctggtg gggttgcttc
    49141 taattcgggg cttagagcta aaataacaga gcttgctaaa gacaatggta ttactgttaa
    49201 gtatcctcct ctaattttat gcacagataa tgcagctatg atagggtgtg caggatatta
    49261 taattttata aatgggaaaa cgcatgatat gagtttaaat gcagttccaa atttaaagat
    49321 aaatcagtag agttagatga atttgtaaat ataaaagtat tgtttcaaaa aagtaagtag
    49381 aagtgaaatt aaatataata aaaataaata tagtatatat taaaacttga agtaaaacta
    49441 tttataatct ttatattcca atgaattgca acaaatttta ggatatacag attgtctttg
    49501 gcttagattt gcttcaagtt taattttttc tatagaaaga ttattttaaa aagtgggtaa
    49561 tatacccttt tttttcattc ttaaaaagtt ttatactaat tatagaaaga ttaaggaggg
    49621 gaagaaatga gtcaaagtaa agaagacaaa ataagaagta ttttggaagc aaaaaatatt
    49681 aaatcaaatt ttcaaaataa agaaaattta agtgaattta atgaaaaaaa agcaagtaaa
    49741 agagcagaag acttattaga tgtatattat aatacattat ctactgctga tatggaattt
    49801 ccatattggt ataatagaga atatagaaaa tcagatggtg atataccagt agttagaaga
    49861 gctaaagctc ttaaagctgc tttttcacat atgacaccaa atataatacc tggtgagaaa
    49921 atagtaatgc aaaagacacg ccattataga ggttcttttc caatgccatg ggtaagtgaa
    49981 agtttctttg ttgctcaagg agaacaaatg agagaagagg caaagaaatt agccagtaac
    50041 acagctgatg aacttacaaa atttgggtct ggtggaggaa atgttactga aagttttgga
    50101 aatgtcgttt caatagctgg taaattcgga atgagaaaag aagaagtacc tgtacttgta
    50161 aaaatggcaa aggaatgggt tggaaagtct gttgaagatt taggttttca ctatgaaaaa
    50221 atgatgccag attatgattt aaaagaaaac ctaatgagta cacttatatg catgtttgat
    50281 tcagggtata cacttcctca aggtagagaa gttataaatt atttttatcc tttaaattat
    50341 ggattagatg gaattataga aatggcaaaa gaatgtaaaa aggcagttgc aggaaatgca
    50401 tctggagatg ggcttattgg tatggaccgt ctatattttt atgaggctgt tattcaagtt
    50461 attgaaggat tacaaacttg gattttaaat tatgcaaaac atgcaaaata tttagaaagt
    50521 atagagacag atttagaagc taaaaaagaa tactcagatt tagtgcagat attagaacat
    50581 attgcacata aacaacctcg tacatttagg gaagctcttc agttgactta tacaattcat
    50641 atagcatcag taaatgagga tgctatatct ggtatgtcta taggacgttt tggtcaaatt
    50701 ttatatcctt ggtatgaaca agatatagaa aaaggactaa ttacaaaaga agaggttatc
    50761 gaattattag agctttatag aataaaaatc acttgtattg actgttttgc ttcagcgggc
    50821 gtaaatggag gagtactatc aggcaataca tttaatactt tatctatagg tggattaaaa
    50881 gaagatggtt ctacaggagc taatgaacta gaagagttat tattagaagc aagtatgaga
    50941 tgtagaactc ctcaacctag tttaactatg ctatatgatg aaaagcttcc agaagatttc
    51001 ttaatgaaag ctgctgaatg tacaaagctt ggttctggtt atccagcatg ggtaaacaac
    51061 tcaaatggaa caacatttat gatgaaacaa tttgctgatg aaggtatgac tgttgaagaa
    51121 gcaagagcat ttgcattagg tggatgtttg gaaacgtctc cagggtgttg gaaacagtta
    51181 actttaaacg gaaaaacata ctctattgca ggtggagcag gtcaatctgc tggctctgga
    51241 gttcacttta tagctaatcc aaaaatttta gaacttgttc ttatgaatgg taaagactat
    51301 cgtatgaata tacaagtatt tgagcctcat aataagccat tagatactta tgaagaagtc
    51361 atagaagtat ttaaagatta ttataaacaa gcaataaatg ttttagaaag agcaaataac
    51421 attgagttag atatatggag aaaatttgat acttctataa taaattcact actaaagcca
    51481 gactgtttag acaaaggtca acatatagga aatatgggat atcgttataa tgcaacatta
    51541 aacgtagaaa cttgtggtac tgttactatg gtcaattcat ttgcagctct taagaaactt
    51601 gtatatgatg acaaagcatt tacaatagag gaaatgaagg atgccatatt aaataatttt
    51661 ggatttaaag atgcattaga agtaggaaat tattctatgg cggaccaagt aaaagtagat
    51721 aagactggta aatatgatgc tatttataag gcctgtcttg atgcacctaa atatggaaat
    51781 aatgatttgt atgcagataa tattcttaaa aactatgagg tatggttatc aaaagtatgt
    51841 gaagaagcac aatctttata tgctaagaag atgtaccctt gccaaatatc tgtttctaca
    51901 catgggccac agggagctgc taccttggct actccagatg gtagactaag tggaacaaca
    51961 tattcagatg gttcagtctc tgcatatgca ggaacagata aaaatggagt atatgcatta
    52021 tttgaatctg caactatttg ggaccaagca gtagttcaaa attctcagat gaatttaaaa
    52081 cttcatccaa ctactataaa aggacaacaa ggaactaaaa aattattaga tttaacaaga
    52141 agttatttaa gaaaaggtgg gttccatatt caatataatg ttgtagattc agaaacatta
    52201 aaagatgcac aaaaaaatcc tgacaattat cgtcaattaa tggtacgtgt tgctgggttt
    52261 actcaatatt ggtgtgaatt aggtaaacca atacaagatg aagtaattgc tagaacagag
    52321 tatgaagggg tgtaaaatat gagaaagcat agtgattgta tgaatttttg tgcagtagat
    52381 gcaaccaaag gaatttgtag attatcaaaa caaatgatta atttagatga tgcagcatgt
    52441 ccagagataa aagtaatgcc aaaatgtaaa aattgtaaaa attttgttga agctaatgat
    52501 gaaggtatag gcaaatgtgt tggcctagag aaagaagact gggtatattc aacattgaat
    52561 gcaattactt gtgaaggaca tgtgtttaat gagtagtcaa aagcaattag aaggcatgat
    52621 ttttgatgta caaagctttt cagttcatga tggtccaggt tgtagaacaa ctgtattttt
    52681 aaatggatgt ccactgagtt gtaaatggtg tgcaaatcca gaaagttgga ctgtaagacc
    52741 acatatgatg tttagtgaat tatcttgtca atatgaaaat ggatgtactg tatgtcatgg
    52801 taagtgtaaa aatggtgcat tgagctttaa tcttgataat aagccagtta tagattggaa
    52861 catctgtaag gactgtgaaa gttttgaatg cgtcaactca tgttattata atgcgtttaa
    52921 attatgtgca aaaccttaca ctgtagatga acttgtgcag gtaattaaac gtgattctaa
    52981 caattggaga agtaatggag gagtaacatt tagtggaggt gagccactac ttcagcatga
    53041 atttttacat gaagtattat taaagtgtca tgaggtaaat atacacactg ccattgaaac
    53101 gagtgcatgt gtatcaaatg aagtttttaa taaaatattt aacgatattg attttgcatt
    53161 tattgatata aagcatatgg atagagaaaa acacaaagaa caaacaggtg tgtataatga
    53221 tttaatatta gaaaatatat caaaccttgc aaattctgat tggaatggaa gattagtact
    53281 tagagtacca gttatctctg ggtttaatga tagtgatgaa aatataagtg atattatatc
    53341 ttttatgcac aaaaataatt tggtagaaat taatctatta ccatttcaca gattaggaga
    53401 atcaaaatgg attcaattag gcaaggaata tgagtattct gataagggtg atgttgatga
    53461 aggtcatcta gaagaattac aagatatatt tttggataat ggtatagctt gttatgttgg
    53521 ccatgagaca gctttctaaa attaaataca agttttaatt aaaaaaggat gttacactaa
    53581 aagctagagt tacatccttt tttatgtggt ttttatgtag aaataattaa gtaaagtttt
    53641 atatgtttta tatattgttt atattgcttt atgtccataa atattgcttt aaatatactt
    53701 tatatatttt ttatagttta aattacagtt agagaatata ttacgtttac ttcattgaag
    53761 cagaagtttt gtggtaaaat actactatga aaaaatttaa tagaatatat tttagaaaca
    53821 actaaaaata atagttagga ggaagacatc tatatgaaca aatcatggag caacttgatt
    53881 attaagaata aagacagaaa agtacgttca gggtggatta ttatagctgt tatggctacc
    53941 ttttacatac ttcagtatta tttaagcatg atacttgtgg aaataataag aaaaatcctg
    54001 gttgctacag gtgacattaa tttaaaaaca aactattttt cagaatatgc taattggtta
    54061 aatgatgttg ggttacagat tgtatttcaa attctaacag attcacttat gattattatt
    54121 cctataattg tatggagatt tattatgaaa aatcctatta gtgaaatggg attacattct
    54181 tatagaatta agaaaaaaga aggatatgta ggaatgttat taggaatatt taattgtaca
    54241 gtaatcttta ttataacaat aacaattggt ggtggtcatg ttacttcatg ggtaccaaaa
    54301 atttcacctt taacagtttc ttggatactg gtctttattt tagttgcttt tgcagaagaa
    54361 attttaaatc gtggtttttt tatggcagtt ttacgaagat gtaaaaatat ttattttata
    54421 atgattgttc catctgttat ttttgggttg attcatatat ggaatcctga tgttactttt
    54481 ttgtctgtaa ttaatattat aattattggg attctttttt catatatgtt tataaaatcg
    54541 tcgaatatat ggatgtgtat aggttatcat tttgcatgga atgtatttca ggggattata
    54601 tatggtatgc ctgtaagtgg acttcaagtt ccagggatta taactacaca aatcactcat
    54661 ggtaatctat taaatggagg aatgtttggt attgaaggtg gaattttgac tacttttgtt
    54721 aatctactta gctttatatt tgtatggtat tactatagag attctaagta tgattttgtt
    54781 agtgataaag taaatgtgac caatatcaag aattagttga ttataataaa ttttaataat
    54841 atcttctaag cttaaagtat tctgtaggat tgttggaaat ttgcaaaaat ataaaaataa
    54901 cttggatatt ctatttactc caagttattt ttatattctt ttgtaaatag aaatggaaca
    54961 taaaaaatta aaacaaccat agatactatt agtatatcat ggtaagtgat agttgattct
    55021 ttagaatttg tgataagaaa agcactatta tttaaaacat gaattatagc acaaaaccag
    55081 atattcttag tttttatata aagatatcct aaaaatattg aaattccaac tatatgtatt
    55141 gaacgtaata ttattcctaa tattggcgct tcaggtgtat ataatgtaaa tattagtggt
    55201 aagtgccaca agctccaaca catacctaat attataactc ctaacttttt accaaattta
    55261 tcaaaaaaaa tattctgtaa aaaacctctc caggcatatt cttcactaaa aaaaatataa
    55321 atagtagtta atgatgttat tggagtaaat ataaatttta tgataccact ataattaggt
    55381 tgagcccctt gtagtattag tataaatgat gttggaacaa ttacagaaat cataaagtaa
    55441 ataattaata aagaacctag tttaatattt tttacaggat ataaattttt tttatttgat
    55501 gaatatatta taattgataa gctacttata aaagttaata ttaggtttat aattataaat
    55561 tcattattat ttattagatt taataactta agaacaatta ataatgaata aataagaaaa
    55621 aataatatga ttagtgaata aaattcatac atatcattat ttaatccttc agtataaaac
    55681 tttgcaatgg caactgaaga tagaggtaat atcatcatga atgatgcaaa gttttctgga
    55741 tcaataaatt tattataata taacagtaaa cctaatgaat aagttaaacc aaagtttata
    55801 aagaaaaaga taataatttg tatttttttt ctttttattt catttttaat aaatactggg
    55861 tttagaatat ttatccatat taa
//
LOCUS       DAGSRM010000016        53400 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.016, whole genome shotgun sequence.
ACCESSION   DAGSRM010000016 DAGSRM010000000
VERSION     DAGSRM010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 53400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53400
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.016"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            72..2342
                     /locus_tag="MJE33_001072"
     CDS             72..2342
                     /locus_tag="MJE33_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBY9647031.1"
                     /translation="MDIIKKLSHGCTLDCHDCCKFNVYTKGNNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSV
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKKYLSSLELSFLIDECGVKENDIREL
                     VDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSQSYGEDREFYVSNISKFIEESLKNTSNKVNYASDELDMALHKVNYISDELD
                     TTSNKVNYVSDELDITSNKVNYVSDELDMTSNKVNYVLDELDITSNKIDYISNELYNL
                     SNKSIKDNIPIKMAVITKSNMLNQLPDLVELERVFSKIEFKVCFDMFITDTATLCDMF
                     IPCTNTLESEDIIYSSMTNPYITYNERAVKPAHKLMDEYYFFMELAKKMGLNDYPFVE
                     KRTYLEKVIEPLKRFDKNLDIEKLKNNYFTIHNPVAWEDKKFETPSGKYELYSESIKN
                     LGISPTPVYISNKYKEIEDKNISFRLLTNHHADTLFSQHFMDKKSIAQAYINRRMAKK
                     VGIEDKDIVILRSKKAKINVQINIDDGVGNYIVKMYVGWWKKHGNPNSLTDTGISDFG
                     GQVTYNESMVEIIRQN"
     gene            2430..2765
                     /gene="spoIIAA"
                     /locus_tag="MJE33_001073"
     CDS             2430..2765
                     /gene="spoIIAA"
                     /locus_tag="MJE33_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HBY9647032.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            2779..3207
                     /locus_tag="MJE33_001074"
     CDS             2779..3207
                     /locus_tag="MJE33_001074"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HBY9647033.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            3230..3994
                     /gene="sigF"
                     /locus_tag="MJE33_001075"
     CDS             3230..3994
                     /gene="sigF"
                     /locus_tag="MJE33_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HBY9647034.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAVKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
     gene            4274..4711
                     /gene="spoVAC"
                     /locus_tag="MJE33_001076"
     CDS             4274..4711
                     /gene="spoVAC"
                     /locus_tag="MJE33_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HBY9647035.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
     gene            4865..5878
                     /gene="spoVAD"
                     /locus_tag="MJE33_001077"
     CDS             4865..5878
                     /gene="spoVAD"
                     /locus_tag="MJE33_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HBY9647036.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQQAIQKAGKKLSDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGDGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGVDISKVYTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            5892..6254
                     /gene="spoVAE"
                     /locus_tag="MJE33_001078"
     CDS             5892..6254
                     /gene="spoVAE"
                     /locus_tag="MJE33_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HBY9647037.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVVFTPKAKP"
     gene            6437..7453
                     /locus_tag="MJE33_001079"
     CDS             6437..7453
                     /locus_tag="MJE33_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBY9647038.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFIGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDKSSKMHEDRTKGYLNAMEEANLKTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAIFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLVNIINKKSTEHIPRKLYPRLIIRNSV
                     KRIK"
     gene            7828..8670
                     /locus_tag="MJE33_001080"
     CDS             7828..8670
                     /locus_tag="MJE33_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBY9647039.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKTRRT
                     SNKL"
     gene            8685..9149
                     /locus_tag="MJE33_001081"
     CDS             8685..9149
                     /locus_tag="MJE33_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647040.1"
                     /translation="MEKTFKTIMEQIKIVLFIYIIICFGQMIALKTQAQDLLIGSVSG
                     FLLVIIAIIIKNFVKKPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTP
                     LLAFAGISVGNQLDILKTLSWKLVVISFIVMTSTYFGSAFISQTVLRLNGML"
     gene            9136..10308
                     /locus_tag="MJE33_001082"
     CDS             9136..10308
                     /locus_tag="MJE33_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBY9647041.1"
                     /translation="MECFKEVVNMNELKAKIKEEIKLLSEEKKTSFEKVSDYLFSNPE
                     LAFEEYKSQKALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPG
                     MGHACGHNIIGTSSVGAGIILKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFN
                     NVDAALILHPSDASMPDDISFAQVNLKFDFTGKASHAAAFPWEGKSALSGVIALFNSV
                     NSMRLHLKDYARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKG
                     AAISTGTNVEIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGN
                     LTHEIPAIQAYIKLKENTATHTDEFAVAAGGEEGRVALIKAIKVLAMCGVDILYSK"
     gene            10352..11287
                     /locus_tag="MJE33_001083"
     CDS             10352..11287
                     /locus_tag="MJE33_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HBY9647042.1"
                     /translation="MILKQVIEVYDILDKANANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKLKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKSPFVFPLSIQDIT
                     PYGNNLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(11658..12383)
                     /locus_tag="MJE33_001084"
     CDS             complement(11658..12383)
                     /locus_tag="MJE33_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBY9647043.1"
                     /translation="MLESKIRTPEEIKRYMLDLKKWLAQERSKPVEEMSDFFSKRIDI
                     YDNVHLANWTEEYAHIADYFDTPLSSLLDIGCGTGLELASIYRKFPNVQITGIDLSKE
                     MMSKLKEKYSDKNIDLILADYFEYPFEQEKYDAALSFETLHHFKYQKKQKIYKKLFQT
                     IKPGGYYVECDYIACCLEEETICLEQYEFKCKENHVPDDVFVHIDIPLTIEHQIHLMK
                     NAGFRAVYVLHENNGTMIIKAEK"
     gene            complement(12401..12967)
                     /locus_tag="MJE33_001085"
     CDS             complement(12401..12967)
                     /locus_tag="MJE33_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY9647044.1"
                     /translation="MPPKPIITKQDIINAAIQLVRENGISSVNARNLAKTLNCSTKPL
                     FRIYTNMDEIKEDIKIELDNYYNSFMESKINDENRLLSQGLAYIEFAQNEKMIFNTLF
                     MNMTMKGSSLQDIIHAKWNRITIENAKKVTGLSIEKSEMLFINFWLYSHGVATQIISN
                     GIDIPLDMVQQLLENAFERFSLDITKTE"
     gene            complement(13878..14276)
                     /locus_tag="MJE33_001086"
     CDS             complement(13878..14276)
                     /locus_tag="MJE33_001086"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBY9647045.1"
                     /translation="MKWKELYNKARGKLNPRTISPFIDAGGVAAAILTDSGNVYTGVC
                     IDTACTLGMCAERNAIANMITNGESKITKLVCVMSNGSVGSPCGACREYLMQLDKDSA
                     CIEILVNVETEKTVKLSELLPDWWGSDRFI"
     gene            complement(14575..17268)
                     /locus_tag="MJE33_001087"
     CDS             complement(14575..17268)
                     /locus_tag="MJE33_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBY9647046.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKEDLE
                     NKLLFFVNTTDDDLDDPTQVDFDMIEKIKSWELISNDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLELKYIKQSEYDEAIKAKIEIKLPQSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDRNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQAT
                     ALSDSRGGYKFEENNKWNPRTTTAGHGSMSLRKALAKSSNTIAVKTAEMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANHG
                     IYVEPSIFTTITTFDGQLLVKNAPEEHKVVEPEVAYVVTDMLESVITEGNGGVAALPK
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKIMEKIHANLTVTEFEVPKNVYFTKINLEDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQPEENNNNNNNTGDNGGGSTPPDNGGNNGGGSTTPPDNGGNNGGG
                     SATPPDNGGNNGGGSTTPPDNGGNNGGGSTTPPDGSGTPAT"
     gene            17482..18180
                     /gene="yunB"
                     /locus_tag="MJE33_001088"
     CDS             17482..18180
                     /gene="yunB"
                     /locus_tag="MJE33_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HBY9647047.1"
                     /translation="MNRIVDRKTKNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTI
                     TVLAETKALELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIA
                     SAIALEIQDELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFE
                     SSGINQTRHRIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKN
                     LGNVLPNTGKNTNK"
     gene            complement(18392..19399)
                     /locus_tag="MJE33_001089"
     CDS             complement(18392..19399)
                     /locus_tag="MJE33_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HBY9647048.1"
                     /translation="MQLKNFHFKNNTLSKKTNLAKSKFTIVLTFLFLLYFFSNNLIYA
                     QNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPFRLGDAIVKVNNAEVDGYSDF
                     ANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNLLSGFATLTYINPDTSEF
                     GAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCINAKRENIIGSFTENTDF
                     GIRGQINNFDISKLKKYKVASLDEVKLGKAQIILQDNFNKCKKYNIEIIDIENQKHPE
                     SKSFKIKITDKKLLAQTGGIVQGMSGTPIVQDNKIIGAVSHAIENDPAVGYGVFIKWM
                     L"
     gene            19627..20511
                     /locus_tag="MJE33_001090"
     CDS             19627..20511
                     /locus_tag="MJE33_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBY9647049.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ADKDENSNKNNKDKNEDKSNSKSNKDKSSEDKSSDNKNEIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKSILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIVQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDAYFK"
     gene            20713..22263
                     /locus_tag="MJE33_001091"
     CDS             20713..22263
                     /locus_tag="MJE33_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBY9647050.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKGKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSKLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQEYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETENLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            22292..22939
                     /locus_tag="MJE33_001092"
     CDS             22292..22939
                     /locus_tag="MJE33_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBY9647051.1"
                     /translation="MKKFLNVLFSICISIVIIVGVINFTVGFKQLYYFDIDYLNISEL
                     SGLSKDDIKLNYDYLIDYNLNKNVSEFKLPTLKSSPQGKIHFEEVRNIFQNINKLAKL
                     LLVVSLVGIILSVKNKNIKILKTTSITLIIMPLLLTVPILLNFEKSFIIFHKLLFRND
                     YWIFNPDLDPVINILPEEFFFHSGMMILILILLVSIVLFVMYKLCKSLSIKIFQC"
     gene            complement(23081..23341)
                     /locus_tag="MJE33_001093"
     CDS             complement(23081..23341)
                     /locus_tag="MJE33_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HBY9647052.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFKYTDKGIKDCSSCILPHKKDNYNYIMSKFQDLVKITSKK"
     gene            23558..24841
                     /gene="hflX"
                     /locus_tag="MJE33_001094"
     CDS             23558..24841
                     /gene="hflX"
                     /locus_tag="MJE33_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBY9647053.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTNESMEELKELTKAAGAE
                     VVGSLIQNKHSVDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYADLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVRLMLPYERGDIFSRIKD
                     KYNVENFEYGESGITLDVDLDEEDFNIYREYILEK"
     gene            24849..25526
                     /locus_tag="MJE33_001095"
     CDS             24849..25526
                     /locus_tag="MJE33_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HBY9647054.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEASEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDRFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDENSLKILQQLKDKNKNFEKEYVLRAYDEAIKSLE
                     NAKNLKK"
     gene            25612..26055
                     /locus_tag="MJE33_001096"
     CDS             25612..26055
                     /locus_tag="MJE33_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBY9647055.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTDCVLAPEFQE
                     GGFYKVKDALEMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            26360..27004
                     /locus_tag="MJE33_001097"
     CDS             26360..27004
                     /locus_tag="MJE33_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBY9647056.1"
                     /translation="MSNYRTLHEFGTDEITIEKSVFIGYAKPIKSEEEALEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVAVVVTRYFG
                     GTKLGAGGLVRAYTKGAKLGLEAGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTVYEDNVEIVVYSRLEDVEKLSEKMIDITSGTGKIVLGEEFYLSEQNGEILI"
     gene            complement(27090..27467)
                     /locus_tag="MJE33_001098"
     CDS             complement(27090..27467)
                     /locus_tag="MJE33_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBY9647057.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGIF
                     LLLVGLFEVKKGFCAKSDKKPLN"
     gene            complement(27467..27853)
                     /locus_tag="MJE33_001099"
     CDS             complement(27467..27853)
                     /locus_tag="MJE33_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBY9647058.1"
                     /translation="MKKTDDLKKKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTASSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            28032..28787
                     /gene="nadE"
                     /locus_tag="MJE33_001100"
     CDS             28032..28787
                     /gene="nadE"
                     /locus_tag="MJE33_001100"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HBY9647059.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYREEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPAQPPKF"
     gene            28870..29589
                     /locus_tag="MJE33_001101"
     CDS             28870..29589
                     /locus_tag="MJE33_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBY9647060.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKEGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            complement(29756..30760)
                     /locus_tag="MJE33_001102"
     CDS             complement(29756..30760)
                     /locus_tag="MJE33_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBY9647061.1"
                     /translation="MATIKDIAKDCGLSSATVSYVLSGKGNERRIPLHTQELILNSAD
                     KLGYKRSTPIKHNNIKIAFYWQDEGFELASTNILQAINHIISLEHLNINIIIRPYKVG
                     HLCDDEYFFANNYYDGIVLTGINADDIEYLVKNKPKIPVVVVNREIPGFSSVSIDNEE
                     TGRIAALHAIQNGKNDIAVVFNPTSFTCMTLRGKSFLKTCKEHGIHLSDSIYYCSNKI
                     DSGYDIAQKMLAKNSLKKVIFCNHDVVALGMMNAFHESGIVIGEDIQIFAASNGPSRL
                     MARSIPPLTVIDLKMEETAERSLRMIIDTVTGRINGIQQQVIYPSIIYRQSSPAQNLL
                     "
     gene            31026..31922
                     /locus_tag="MJE33_001103"
     CDS             31026..31922
                     /locus_tag="MJE33_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HBY9647062.1"
                     /translation="MSKVNIFEVGPRDGFQNLKEFLETDKKLKIIDGLIASGIKQLEI
                     SSFVSPKAIPQMKDAKEVATYCVEKYPDVKLYALVPNLYGAKVAWECGIRDISYVISL
                     SETHNKKNINRTHEESIDELKKIMNTYPDMNICIGMATVFGCPFEGLPKIEDAIRFAK
                     CLWENGIREINLADTIGIADPKQVRETISALVEALPECRWQVHIHDTRNMGMVNTLVA
                     IESGITTVQSTLGGLGGCPFAPGASGNTATEDLVYMLERMGYDTGVDFVKLLELSKYQ
                     KSFVNGVYSGHHVNIKSDSCNC"
     gene            32059..33321
                     /locus_tag="MJE33_001104"
     CDS             32059..33321
                     /locus_tag="MJE33_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647063.1"
                     /translation="MDIIALLLFLGVIIIAFVRKNNAGILALAIGVIAVRIFGMDDKE
                     LYGAISNSMFTTLVGITLLFAIVNSTGALKLVAEKIVAASGKRVWLIPIAVFIAGFVV
                     AGVGPGAIPALAIIPAMAVPVAITVGYNPVMLALIGECGLMAGRMTPITPEGQIITTV
                     AEGVGIENVMPTILACQTLSTIVFSIVLFIIFKGYKLKKPINVIDSKQIEKFNIKQII
                     SLISIVVMIAFIIIFDMNVGLAAFAVSAVLIFFNIADEGECIKSIPWSTIVMVLGVGA
                     MMTIVDAAGGIDLMSNALSSLMNSKTATPIMSISAGLMSMVSSALAVVYPTMMPMCVD
                     IAKAVGGVDPLALIAAVGVGGSLAGVSPLSTGGALILAAMGSSKKDFNKEEQNKVFVQ
                     LFIMSAVGLLVIAVVSILSFNAITHLMN"
     gene            33397..34590
                     /locus_tag="MJE33_001105"
     CDS             33397..34590
                     /locus_tag="MJE33_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HBY9647064.1"
                     /translation="MKKGALSNIKVLDLTRVLAGPYCTMMLADFGADVIKIEMPRKGD
                     DTRGFGPFVNGESLYYANINRNKKGITLNLKSEEGKNIFLEMVKHADVVVENYRPGVM
                     EKLGLGYDELAKVNNQIIYGAVSGFGCYGPYSERPGYDIIAQAMGGLMSITGQEGGEP
                     TRVGNAMGDVLGGMNLTIGILTALNARKIIGKGQRVDVSLVDSVVSSLETGIQRYFDS
                     GQPELMGNRYASAYPYDSFKASDGSFVIGCGNDKLFEKLCKKVLFREDLLIDERFKTN
                     LNRCEHYKALKPEIEKWSVKHTIKEAVDLISEAGVPAAPIYNLDCVTTDKHIAKAREM
                     IVDISHPIIGNMKVNGNPVKLMTTKAKIDMPAPSLGESNEEIYNGWLGFDSDKIKDLK
                     ENGVI"
     gene            35219..36013
                     /locus_tag="MJE33_001106"
     CDS             35219..36013
                     /locus_tag="MJE33_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBY9647065.1"
                     /translation="MESVMENLNNLKVEVKDKVCVITINRPKALNALNSDTLRELSQV
                     IDVVSENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGIFDKIEALEK
                     TVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTG
                     IAKELIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVI
                     NKGIDMDLKNALELEKDVAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            36108..37448
                     /locus_tag="MJE33_001107"
     CDS             36108..37448
                     /locus_tag="MJE33_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBY9647066.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFIVGSTILYKMVSKEKANGGHFIA
                     HESDIFDEAEATNSKRENGPNGVIALIPLIVSILIINVKVNGKAIVPIEVGVFIGALL
                     VYLLLNKYQDNTKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            37585..38817
                     /locus_tag="MJE33_001108"
     CDS             37585..38817
                     /locus_tag="MJE33_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HBY9647067.1"
                     /translation="MLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFGDP
                     GRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSETGIEIMDKLLSEANVFVSSYR
                     TGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDIAE
                     KDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSLLA
                     STQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIKEE
                     KFASSVESKKNATELINILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQAI
                     ANNYIYEHTSKDGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSDIE
                     TLIESGIVTMNKEACEIC"
     gene            38857..39999
                     /locus_tag="MJE33_001109"
     CDS             38857..39999
                     /locus_tag="MJE33_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBY9647068.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGADIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGVGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASRTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            40010..40804
                     /locus_tag="MJE33_001110"
     CDS             40010..40804
                     /locus_tag="MJE33_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBY9647069.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKITVLTLADLDVDTTQI
                     GLKGSPTNVYKSFVPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            40815..41837
                     /locus_tag="MJE33_001111"
     CDS             40815..41837
                     /locus_tag="MJE33_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBY9647070.1"
                     /translation="MMRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLA
                     DQLGKELAVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERK
                     KPEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMAT
                     IVCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEILPQEKKSV
                     NLTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGT
                     TVRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSICDYGIVGDLYEIIPEM
                     IESLNR"
     gene            41936..43312
                     /locus_tag="MJE33_001112"
     CDS             41936..43312
                     /locus_tag="MJE33_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBY9647071.1"
                     /translation="MKEQWYKDIFARVLSMTDDGFIVVNTSGVIIDINDKYCDFLGKE
                     RKDIIGQNIQSIIPNTKMLDVMKNKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENRDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(43644..43988)
                     /locus_tag="MJE33_001113"
     CDS             complement(43644..43988)
                     /locus_tag="MJE33_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647072.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIERFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
     gene            44476..45486
                     /locus_tag="MJE33_001114"
     CDS             44476..45486
                     /locus_tag="MJE33_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBY9647073.1"
                     /translation="MHEDKNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTE
                     EICLEAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEEPTEEICMLAINQDGTAL
                     KYIENQTEELCLRAVEKNGAALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEA
                     VKHCSYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGMALEFVKEQ
                     TVGICLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALYDNNEPF
                     AKRYVRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLG
                     VF"
     gene            45622..46110
                     /locus_tag="MJE33_001115"
     CDS             45622..46110
                     /locus_tag="MJE33_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBY9647074.1"
                     /translation="MKITVLYQTRSGNTERVAKLIEEGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTIVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FA"
     gene            46286..48214
                     /locus_tag="MJE33_001116"
     CDS             46286..48214
                     /locus_tag="MJE33_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBY9647075.1"
                     /translation="MVWHIEYEIFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFIISNILGSFCLNNIDKIPIFITFLLNQIYLLLLPIPAALVSFYVMAIIYQDIRYMK
                     KRLLFLSIPLIICIILSITNPFTHILFGLSKETGYIRGCGYVATFASFFFYAIYSAFL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALKEV
                     GKYLIQIHDVNHVYRIGGDIFSIVVNSQLGIEPDDIIRKIEERFKKPWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKKKHVIKNCL
                     KEALKNDGFEVYYQPIFSNSRGRFTSAEALLRLEHKELGFIPPDEFIPIAEQTGLINS
                     IGLVVFEKVCRFIASDEFEDLKLDNIAVNLSVVQCMQKNLADDLLYLMNKYNVSPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIAYSGVTNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PVPEDVFKNLLKENTFTS"
     gene            48391..48801
                     /locus_tag="MJE33_001117"
     CDS             48391..48801
                     /locus_tag="MJE33_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBY9647076.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
     gene            48823..49125
                     /locus_tag="MJE33_001118"
     CDS             48823..49125
                     /locus_tag="MJE33_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HBY9647077.1"
                     /translation="MNKKIVAVCESERSLNSLKTAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(49342..50034)
                     /locus_tag="MJE33_001119"
     CDS             complement(49342..50034)
                     /locus_tag="MJE33_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBY9647078.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMIAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(50060..51247)
                     /locus_tag="MJE33_001120"
     CDS             complement(50060..51247)
                     /locus_tag="MJE33_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY9647079.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRLPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSIFYMVCAVASSGFASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            51583..53382
                     /locus_tag="MJE33_001121"
     CDS             51583..53382
                     /locus_tag="MJE33_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBY9647080.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKKVYDEVIKIVPQSNNIGKKNESQEKQGIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSSIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKAGYDVTTPVIVNNTFD"
BASE COUNT        19961 a         6439 c         9644 g        17356 t
ORIGIN      
        1 agaaagttta ataaattata gtatatatgt taatttttaa taaggtaaga aagtagaaag
       61 aaggatattt tatggatata ataaaaaaat taagtcatgg gtgtacatta gattgtcatg
      121 actgttgtaa attcaatgtt tatactaaag gaaataatgt agtaaaaatt gaaggagata
      181 aaaatcatcc ttatacaaaa ggttttatat gcaaaaaagg gatggcccat ttagatagat
      241 taaatcataa agatagaata aagactccta tgcttaaagt tgatggagta tggaaagaga
      301 tttcttttga taaagctata gaaataatgg cagaaaagct tacatactat aaagaaaagt
      361 atacatcaaa gtctgtcatg cattatgacc aatatggaag tgggtctgtg ctaaaataca
      421 taggagatat attttttaat ttttatggag gtgttagtag acataagggg ggtccctgtt
      481 ggagtgcagg gatgcatgct caaaaatatg attttggagt agctaagagt catgctatag
      541 aagatatgtt aaatagtaaa agtatatttg tgtggggaaa aaatccagca tatactacaa
      601 ttcatactat gcaaataatt aaaaaagcta aagaaaaagg gataaagata gtagtgattg
      661 accccatata tacaaaaaca gctcaaatag cagataagta tgtacaagta aatcctggaa
      721 cagatggagc tttagctata gctatggcaa aaattatagt agaagataaa ttatatgatg
      781 aagaatatat aaactcgtat gttattggtt ttgaagaata taaaaaatat ttatcttcat
      841 tggaattaag ttttttaatt gatgaatgtg gtgttaaaga gaatgatata agagagttag
      901 tggatttata tactaacaaa tattcaagta taaatgttgg atatgggctt caaaaatata
      961 aaaatggtgg aaacacaata agagcaatag atgctttagg tgctataact ggtcaaatag
     1021 gatttagtgg tggaggggta aactatgcta ataaagttta tccgagtgtt ataaattctg
     1081 acccttacaa tagccaatca tatggtgaag atagggagtt ttatgtaagt aatatcagta
     1141 aatttataga agaatcttta aaaaatacat caaataaagt aaattatgca tcagatgaac
     1201 ttgatatggc attacataaa gtaaattaca tatcagatga acttgataca acatcaaata
     1261 aagtaaatta tgtatcagat gaacttgata taacatcaaa taaagtaaat tatgtatcag
     1321 atgaacttga tatgacatca aataaagtaa attatgtatt agatgaactt gatataacat
     1381 caaataaaat agattatata tcaaatgaat tgtataattt atctaataag tcaatcaaag
     1441 ataatattcc tataaagatg gctgtgataa ctaaaagtaa tatgctgaat caactgccag
     1501 atttagtaga attggagagg gtgttttcaa aaattgaatt taaagtatgt tttgatatgt
     1561 ttataactga tacagctact ttatgtgaca tgtttatacc atgtacaaat acactagaga
     1621 gtgaagatat aatttatagt tcaatgacca atccatatat aacctataat gaaagagctg
     1681 taaagccagc acataaattg atggatgaat attacttctt tatggagcta gctaaaaaga
     1741 tgggattaaa tgattatcct tttgtagaaa agagaactta tttagaaaaa gtgattgaac
     1801 cacttaagcg ttttgacaaa aatctggata tagaaaaact taaaaacaat tactttacta
     1861 tacataaccc tgttgcttgg gaagataaga agtttgagac tccatctgga aaatacgagc
     1921 tttattcaga aagtataaaa aacttgggaa taagtccaac acctgtatat ataagcaata
     1981 aatataaaga aatagaagat aaaaatattt cttttagatt attaactaat catcatgcag
     2041 atactttatt tagtcagcat tttatggaca aaaaatctat agctcaagca tatataaatc
     2101 ggagaatggc taaaaaagta ggtattgagg acaaagatat tgtgatattg aggtcaaaaa
     2161 aagcaaaaat aaatgtacaa ataaatatag atgatggagt aggtaattat atagtaaaaa
     2221 tgtatgttgg ttggtggaag aaacatggaa atcctaatag tttaacagac actggtatat
     2281 cagattttgg gggtcaggta acatataatg aaagtatggt agaaataata cgacaaaact
     2341 agttaaattt cattgaagga ataaaaatat aattatagaa ttgattaaaa gttactattt
     2401 ttacaaaact attgaaggag ggatttttga tggtaaatta ttccttagaa cataagaatc
     2461 tatacataga atttatgtgc tcagaactag accaccatgt agccaatgaa ataagggaag
     2521 aaatagataa cttattaagt gtaaatcaag taaaaaatgt tgtatttaat tttgaaaata
     2581 ttaattttat ggattcttca ggtataggcg tcatcatagg tagatataaa aagatttcta
     2641 atgaaggggg acgagtttca gtaattaata taagttcacg agttaagaag atttttgatt
     2701 tatctgggtt aaataaaatt attggcattt atgatacgta tgaagaagct ctaagttctt
     2761 tgtagggagg agtatgtgat gaataatatt atggaagtta aattttcggc aatatctgaa
     2821 aatgaaagtt tagcaagagt tatagtagca tcctttgcag ctaagttaga cccaacagta
     2881 gatgagttag tagatataaa aactgctgtc tctgaggctg ttacgaatgc tataatacat
     2941 ggatatgaag aagacagttc taagtttgtt ttcttaagat gtgagataga aggaaatact
     3001 ataaagatta tagttgaaga tgaagggtat ggaatagaaa atgtggagaa ggcaatggaa
     3061 ccactttata catcaaaacc agaacttgat agatcaggaa tgggattcac agtaatgaaa
     3121 agctttatgg acgatgtaga ggtaagttct gtaaaagata atgggactag aattgaaatg
     3181 actaaaaaaa taaatgtgcc taaataaagt ccaaaagtga ggttttatta tggaagtaac
     3241 tgttgccaga gaagaaaaaa aacctcttct tagtcatgaa gagactctag aacttattga
     3301 aaaagttcaa aatggagatg aagaggcaaa agaaatatta atatcaagca atttaggtct
     3361 agttaggagc gttgtatcta gatttttaaa tataggatat gatagagaag atttatttca
     3421 gttaggttct ataggcttaa taaaatcaat atataaattt gacccaaaat ttaatgtaaa
     3481 attctctact tatgcagtac caatgatttt aggtgaaata aagagatact taagagatga
     3541 tggtatgata aaagtatcaa ggtcattaaa gcaaatagct gtaaaggcaa agatggaaag
     3601 tgaagcttta actaaaaaat taggtagaga gccgagtata gaagaattag ctaaagcaat
     3661 agatgtagaa aaagaagatt tagtaatggc tatggaagct aatttcaatg ttgagtattt
     3721 gcaaggagtt attcatgaag aggaaggttc tccaatatgc cttattgata aaataagcat
     3781 gaaaggagaa agtgaagaag aaaaagtagt ggataatata ttgttaaaag acatacttgg
     3841 aagactggat aagagagagc gtcaaataat agttctcaga tactttgaag acatgactca
     3901 aagtgaaata ggagagatgc tcaatatatc acaagttcaa gtatctagga tagagaagaa
     3961 agtattgtct aagttaaaag aatatatatc gtgataatac ttatgatatg tagaataaca
     4021 aaatataata aatatattac taaaataaaa tgtaataact ttttatttta agtttttaaa
     4081 ttattaaaag atattaaaat aataaaaagc attaaaaaag taaaaatata aaaacaaggt
     4141 gtctttatgg aaaaatagat tttttcttta ttaggcatct tttttatttt ttgaccaaac
     4201 tttagatagt caaaataaat aataggttta tttttttaaa attaacaaat aatactttag
     4261 agaggtgttt agtatggata aaaattataa aaaatatgta gaccaaataa gcccaaaacc
     4321 tacatattta aaaaactata cacttgcatt tattgtaggt ggaattattt gtatgatagg
     4381 gcaagcaata aatgatttgt atatgaaagt tggaggtctt gataaactag gtgctagttc
     4441 tgcaacatca ataacattaa tatttatagg ggcattttta actggattag gagtctacga
     4501 cctaatagga aaaagagctg gagctggttc tataattcca ataacaggtt ttgctaactc
     4561 aatagtatct ccagcaatgg agtataaaag agaaggctat gtattaggtg taggtgcaaa
     4621 cttatttaaa attgcaggac cagttttagt ttatggtata ggttcttcta ttttatgtgg
     4681 aattatttat tatattttta agatgtttta gatgattttt atttaattta aattttatta
     4741 attatgaaga aggtataaaa tctgaaaata gctactaaag taactaattg aaaaataatt
     4801 gtgcagattt aattaatatt aaaaatttga atagtaaaat gcaaaattaa aaaggtgtga
     4861 aaatatgaaa aataaaagaa ttggaaaaag aacagtcaag ttagaaaata aaccaaccat
     4921 aatatcaact ggaactatag taggtcctaa ggaaggtgaa ggaccactaa aggactattt
     4981 tgatatgatt atgactgacg atttatatgg tgaaaagact tgggaattag ctgaaagtaa
     5041 aatggtagag actgcatctc aacaagctat ccaaaaggca ggtaaaaaat tgtcagatgt
     5101 aaattatatg ctaggtggcg atttaataaa tcaaatagtt ccagcatcat ttgcagcaag
     5161 agaactggct attccatttt taggtatata tggagcttgt tctacaatgg cagaaggtct
     5221 ttgtataggt tctatgctta tagatggtgg ttttgctgat ttagtactgt caggtacatc
     5281 aagtcactat tgtacagcag aaagacaatt tagattccca cttgaacttg gaaatcaaaa
     5341 accaatgaca gctcagtgga cagttacagg agcaggaagt gtattattag ctcctaatgg
     5401 agacggtcca aaagtaaagt atgttactgt tggtaaagta atagatgagg gtattgatga
     5461 tggtaataat atgggaccag ctatggctcc agcagcaata gatactatct actcttactt
     5521 tgaagatact aatgatgacc caaatagctt tgatataatt gctactggag atttaggtac
     5581 tcttggaaaa cagatagcag aagactttct aaaagaaaag ggtgtagata tatcaaaagt
     5641 ttatactgac tgtggtatag agatgtttaa ccttaaagaa caagatgtcc actgtggtgg
     5701 aagtggatgt gggtgttcag ctacagtgtt tgcaggatat atatatgata agttgagaaa
     5761 aaaagaattt aacaaagtaa tgcttgtatc aacaggagca ctactttcac cgacaagtac
     5821 actacaaaag cagacaatac catgtgttgc acatgcagta gtaatagtaa atgagtagga
     5881 agtgatataa tatgttaatg gactatgtaa gagtatttat tgtaggtgga attatatgtt
     5941 taatagccca agtaatgatg gattatttca aaatgcaaac tccatatgtt atggtaacgt
     6001 atgtaacaac tggtgtagtt ttaacttttc taggacttta tgagccatta gttaagtttg
     6061 cagcagcagg tgcaacagtt cctataatag gatttggata ttcacttgct acaggagtaa
     6121 taaaatcaat tcaaagtgat ggttttttag gtattttttt aggaggtaca actgctacag
     6181 ctggtggaat agcagcagct atattctttg gatatattat gtcagtagta tttactccaa
     6241 aagcaaaacc ataaatcttt attaaaagaa taataagttt ttgaaataat aactttttat
     6301 aaagcaggtg gatatctttt agattccacc tattttttat ataaagaagc gtttacataa
     6361 ttgaagttga caattattta caacatagtg tagtattata ttatcaaaat aaaatgaagt
     6421 gaagtagggg ataaagatgg taactattaa agatattgca aggaatctag gtatcagtta
     6481 ttcaactgtt tctaggtgct taaacaataa tcctaatgta tctgaaaaaa caaaaaagaa
     6541 agtggtagaa gaggcaaata gactagggtt ccattttaat gtaaatgcaa gaaatttagc
     6601 aaaaaaagaa actaacagga taggggtaat cttttcaaat aattttaatc atcaagatac
     6661 tagaaagttt tttagtgata taatggatag ttctataaat tctattgaga ctaataaata
     6721 cgattttata atacagccta ataataacat tagtggtgat agtaatgtat ataaaatggt
     6781 aaatggacaa atggttgacg gattagtaat tgtgtctcaa tcaataaaaa aagaagagta
     6841 tgatttttta aaagacaata attttccaca tgtatttata tattttaaac catcttttat
     6901 aggagaagta gataatttct tttgggacga taacgtgtat ggaggttata tagctaccaa
     6961 gcatttaatt gaccatggtc ataaagatat aataacagta acttcagatg ataagagttc
     7021 aaaaatgcat gaagatagaa ccaagggata tttgaatgct atggaagaag caaatttaaa
     7081 gacagaagtc attaaatcta aaatggattt tgaatctcaa gttgaatttt tagaaaaata
     7141 cattgataaa ataaaaaaag cttcagctat atttgtacaa caagatgtac cagcgatttc
     7201 tataatacaa gagttaaaaa ctacttatgg tataaatgta ccagaagata tttccataat
     7261 agggtataat aatattgaat taatatcata ttttaggtcg catttatcaa caatagatga
     7321 cccaagagag caagttataa aaaatggagt agatagttta gtaaatataa taaataaaaa
     7381 gagtacagaa cacattccga gaaaattata tccaaggtta ataatacgaa atagcgttaa
     7441 aagaataaag tagtaagctt ttaagtatca aacgtttatt aaaaatatta tagattatgt
     7501 gaagtttttt aacaaataat ataaataaaa attaaaagga gtaggtgtat ataagtacat
     7561 tgtactcctt tatttattga gatagttttt ataatatgaa tgaataattt tattcaatta
     7621 tttgaaaatc cgttatttta acaatataaa ttaataaatt atttgtattt tatatcatga
     7681 atctataata tttttttgat tcataactaa aagtatataa aaatgatgaa taaatatata
     7741 ttatacatta tcaatgatag gttatataat aaaatcacta acaattatat tataagaaaa
     7801 ttataaaaat tacaaggagg tatagaaatg aaagaaaaag cagggattag aaatgccata
     7861 atagcagcct ttataatcac aatattggca gaattaattg gacctatttc atttaaagtt
     7921 atgggaatta atgtaacttt acttagtata ctatgggcta tattcatagg aatggctgtt
     7981 tctcctcatt tattagggag agttatacca gccttaaaaa agtttatagg ggataatgaa
     8041 ataaatgtat ctccatattt attatctttg acactatatc cattagggat aatgtttgga
     8101 attaatgcag ggcctaaagt tggtatatta cttcaagcag gaccagcact tttattacaa
     8161 gaatttggtc atatgggtac aatgttgata gcattacctg ttggagtagc attaggtctt
     8221 ggaagaagtg cattaggtgg tactttttca ttatgtcgtg acacagcact aggaattatt
     8281 ggagataagt atggattaaa ctcaccagaa ggtataggaa cactaggaac ttacataagt
     8341 ggtagtatat ttggaacatt attattttca tttttagcac cagtgggtgt acttttagga
     8401 tttcatccat atgctttagc aatggcatca ggaatgggta gtggttcaat gatgggagct
     8461 gcaacagcat cattaatgaa tacggttcca caaatgtcag agcagatttt agcatattca
     8521 gctactagtg gattgattac agctgttact ggaatatata tagaattatt cttatcttta
     8581 ccagtagcca actactatta tagcaaagta gctccagtta ttgaaagatt tagaggaaga
     8641 aaaacaagaa gaacaagtaa taaattgtaa aggaggatat atatatggaa aaaactttta
     8701 agactattat ggagcagata aaaatagtat tgtttatata tattattatt tgctttggac
     8761 aaatgatagc acttaagact caggctcaag atttgttgat tggaagtgta tctggatttt
     8821 tgttggtgat aattgctatt ataataaaga attttgttaa aaaacctaat ttaccaggtt
     8881 ttgcatgggc tacattagtt gcattcgttt tatcgcttcc aatatctcca gtgggggata
     8941 tcatagcaac aaatgtaggg aaaatcaatt ttatgtctac agtaacacca ttattagctt
     9001 ttgcaggaat ctctgtaggt aaccaattag atatattaaa gactttgagt tggaagttag
     9061 tagttatatc atttatagtt atgacatcta cttactttgg ttctgctttt atatctcaaa
     9121 cagttttaag attaaatgga atgctttaag gaggtagtaa atatgaatga attaaaagca
     9181 aaaattaaag aggaaataaa gttattatct gaagagaaaa agacatcatt tgaaaaagtt
     9241 tctgactatt tattttcaaa tccagaatta gcatttgagg agtataagtc acaaaaagca
     9301 ttatgtgatt tgttagaaga aaatgaattt aatgtaacta aaggtgtagg aggattagaa
     9361 acatcttttg aagctgtgta ttcaaatgga actaatggga aaacagttgc ttttttagct
     9421 gaatatgatg cacttccagg aatgggacat gcatgtgggc acaatatcat agggacaagt
     9481 tctgttggtg caggtatcat attaaaagaa atcatgaaaa aacataatat agaagggact
     9541 gtaaaggttt ttggaacacc tgcagaagaa agagtaggtg gaaaaataac tatgattaaa
     9601 gaaggtgtat ttaataatgt agatgctgca ttaattttac atccaagtga tgcttcaatg
     9661 cctgatgata tatcatttgc tcaagttaat ttaaaatttg attttactgg taaggcatca
     9721 catgctgctg cttttccttg ggaagggaaa agtgccttaa gtggagtaat agcactattt
     9781 aacagtgtaa atagcatgag attacatctt aaagattatg caagagtaca tggaattata
     9841 actgatggag gtagcataca taatataatt cctgaaaaat ctacagctat atttaatgta
     9901 agagccttaa gtatagaata tttaaatgag atttgtgaaa tgcttaaaaa ttgtgctaaa
     9961 ggagctgcaa tttctactgg aactaatgta gaaatagttc aattagatga aatatacaaa
    10021 gagattaaga atgattctga attagtgaat atagttagac aaaattttga agttttgggt
    10081 gaagactatg ttgaaagaga tttaagtcaa ggtataggtt caactgatac aggaaattta
    10141 actcatgaga tacctgcaat acaagcatat ataaaattaa aagaaaatac tgcaactcat
    10201 acagatgaat ttgctgttgc agctggtggt gaagaaggca gagtagcact tataaaagca
    10261 attaaggtgt tagcaatgtg tggagtagat attttgtata gtaaataata tataatttta
    10321 aaatatatta agaaaggaaa tcggattaga catgatatta aaacaagtaa tagaggttta
    10381 tgacatttta gataaagcaa atgctaatgg ggaagaagta aaaacttact tacaaggtta
    10441 tggtgaagta gatgtaactg taaaagaatt gtcttcaagt aaaggctcaa cagatttagt
    10501 aaagcttaca attcctggga aaaatggaaa attaaaaggt ggagatgctc ctactcttgg
    10561 cattttaggt agactaggag ggattggagc aagaccagaa gttataggtt ttgtttcaga
    10621 tggtgatgga gcattggtag caattgcagt ggctgctaaa cttttggata tgcaacgaaa
    10681 aggggatata ttagatggtg atgttgtaat ttcaacccat atttgcccag atgctccaac
    10741 taaagaacac tatcctactc cttttatgga ttctccaatt gatatgatga ctatgaatga
    10801 aaatgaagtt gatagtagtt gtgatgcaat actttctata gatactacta aaggaaatag
    10861 agtaatcaat actagaggat ttgcaatatc gccaactgtt aaagaaggct acatattaaa
    10921 aactagtgat aaccttttag atattatgca gactgtaaca gggaaatctc catttgtatt
    10981 ccctttgtct attcaagata taacaccata tggaaataat ctgtatcact taaatagtat
    11041 tttacaacca gcagtagcta cagatgcacc aatagtagga gtagcaatta caacagagat
    11101 gcctgttgct ggatgtgcta caggtgcaac taattttagt gatttagatt cagctgggag
    11161 atttgcaatt gaagttgcca agctatttgg tagaaataag tgtgatttct atgataaaga
    11221 agagtgggaa atgcttatta agagatatgg caaattaaat agatttcaaa catttggtat
    11281 acagtaatta gaaaaatatt aagtttatat aatttgtgat ataaatatct atactatgta
    11341 aacaactaga aacaaaagat atagtttaag acttaaaaaa taaaatattt atttgattac
    11401 ataaataagg agtgttctat ttaggtaagc gccaataagt agtataacat taaagctaac
    11461 ctaattgttg atatacaaga ttgaaatgaa aacagtgcgt attataagaa aaggttacgc
    11521 actgtttttt tcgtcctgtt atatagcaga acactagttt ttaagggtat gagtataaaa
    11581 cttttttaaa tttcatatct acgaattttg tagattgtcc ataagcgata tgcaataatt
    11641 aggaaaatat tttgagttta tttttcagct ttaataatca ttgtgccgtt gttttcatga
    11701 agtacataca cagctctaaa gcctgcattt ttcattaagt gtatttgatg ttcaatagtc
    11761 aaaggaatat caatatggac aaaaacatca tcaggcacat gattttcttt acatttaaat
    11821 tcatattgtt caagacaaat tgtttcttcc tctaaacaac aggcaatata atcgcactca
    11881 acataatatc ctccaggttt aattgtttga aaaagctttt tatatatctt ctgctttttc
    11941 tgatatttga aatgatgaag cgtttcaaat gagagggcag catcatattt ctcttgctca
    12001 aaaggatatt caaaataatc tgccaagatt aaatcaatat ttttatctga atatttttct
    12061 ttaagcttac tcatcatttc ttttgataaa tcaatgcctg taatttgtac attaggaaat
    12121 tttcgataaa tcgatgcaag ttctagacca gtaccacaac caatatcaag taaagaagat
    12181 agtggagtat caaaataatc tgctatatgt gcatattctt ctgtccaatt agcaagatga
    12241 acattatcat aaatatcaat tcgctttgaa aagaaatccg acatttcttc aaccggttta
    12301 cttctttctt gcgctaacca ttttttgagg tcaagcatat atcttttgat ttcttcaggt
    12361 gttcttattt tactttccaa catataaaac agtcctttct ttattcggtc tttgttatat
    12421 ccaatgaaaa tcgctcaaaa gcattttcaa gtaactgttg taccatatct aatggaatgt
    12481 ctatgccatt ggaaataatt tgtgtagcta ctccatgaga ataaagccaa aagttaataa
    12541 atagcatttc tgatttttct atagataagc ctgtaacttt tttagcattt tctatagtaa
    12601 ttctgttcca ttttgcatga ataatatctt gtaaggatga acccttcatt gtcatattca
    12661 taaataaggt attgaaaatc attttttcat tttgagcaaa ttcaatgtat gcaagtcctt
    12721 gacttaacag acgattttca tcatttattt tactttccat aaaagaatta tagtaattgt
    12781 ctagttctat tttaatatct tcttttatct catccatatt tgtgtaaata cgaaaaaggg
    12841 gttttgtaga gcaattaagg gtttttgcta agttacgagc atttacgcta cttattccat
    12901 tttctcttac caactgaatt gcagcgttaa taatatcttg ttttgtaata atcggttttg
    12961 gtggcatctt ttcatcctcc tttcggtaac ttaagttgcg caactggtgt tgttattata
    13021 acaaaggcat ataactgtgt caatagactt caaagaaaag ctacagacat cgagtgtaat
    13081 atgatttatt tcaataaata ccataatatg tgtacttata tcttaaaaag gagataaaca
    13141 gtaaaatgta gtttagttaa atttgaaact tcgtcaaagc ttctttgtac gataagacaa
    13201 cgtacaaatc attgtatggg taacagagaa agaactgcgt tgattgagga tgaaatctca
    13261 catgatacga tgtaattata aaaaaaggag cgtttcaaag tgattttttt aatcatcttg
    13321 agacaactcc ttatattttt tatttaaatt ctatttttta ttgcctgtat agctattttc
    13381 tccatcataa ggtggcatga ccttgaacag cgtggttttg taaagttggg caccacaaaa
    13441 caaaaaatca acaagccctt gtgtggtctg ctgacgtgtt tactatacta tactttatcc
    13501 aaaaccgcaa gcactatggg aggctataag gcggcggcca ttcaggggcg atcccctcga
    13561 aaccgggata gggttcccat gtgggacata ataaagagcg ccccggaggt atcaactagc
    13621 atctggggcg ctctttatta tgccccgtgg gaaccatgcc cacgaagcag ggcgtacaac
    13681 ggacagatag gtcctaccct cctgacaaac tggaatctcc gaaaacaatc ataacaatca
    13741 tggcaagatt atatacactc ctgtaaaata tttttgtgtg taccatgaag tactgcttgc
    13801 cactattgag aacggataaa agggaagtac gtattatacc aataaggtgc atgaaacaaa
    13861 atactcattt gataaagtta aataaacctg tcggaacccc accaatccgg caaaagctca
    13921 ctcaatttaa cagttttttc tgtttcgaca ttaactaaaa tctcaataca agcactatcc
    13981 ttatcaagtt gcattaaata ttcacgacaa gcaccacatg gtgagccaac actcccattg
    14041 ctcatcacac aaactaattt agtaatttta ctttcgccat ttgttatcat attggcgata
    14101 gcatttcttt ctgcacacat tcccaatgta caggctgtat caatacacac accagtataa
    14161 acatttccgc tgtcggtaag gattgctgcg gcaaccccac cagcatcgat aaaaggtgaa
    14221 attgtgcgtg gatttaattt tcccctagcc ttattataaa gttccttcca tttcatcagg
    14281 ttgatcctcc tgttgtatcc atataaataa tcttttatac gccttttccg cctaaggcac
    14341 acaacacatt actcgctttt ttcattctaa tattcgtgcg tgaaaatgtc aaacggttta
    14401 cttcgtttca ttacttcata ctgctgttca taaccctgtt tgttcacttg ggcaccatta
    14461 gagcaccaat ttacccgttt cagggttgta gcgaacacag cagaaatgct catttcatgg
    14521 gcttttctgg ctagaaaaaa aattttatcg ctgccagata tttactattt tttattaagt
    14581 tgctggagtt ccactaccat caggaggagt agtagagcca ccaccattat ttccaccatt
    14641 atcaggagga gtagtagagc caccaccatt gtttccgcca ttatcaggag gagtagcaga
    14701 gccaccacca ttatttccgc cattatcagg aggagtagta gagccaccac cattgtttcc
    14761 gccattatca ggaggtgtag aaccgccacc attatcacct gtgttattat tgttattatt
    14821 attttcttct ggttggttat tgtctggttt ttttgtatct tcagatgatg gttgagaact
    14881 tactttagta ggagcagtac cttcaataaa tgctgcttta gagccagaag attgctttcc
    14941 tccatcctct aaattaattt ttgtaaaata tacattttta ggaacttcaa attcagtaac
    15001 cgttaaatta gcatgtattt tttccataat tttttcccat agttttgcag tactagtact
    15061 tccatgtggt acttctctac gttttatagt gttaccagaa tcatcttttc taccagcatc
    15121 atctccaata taagttgcac caacataata aggagtgtaa ccgacaaacc aagcatcata
    15181 tgcactattt gtagtacctg ttttacctgc tacaggcatt ccctttggaa gtgcagcaac
    15241 accaccatta ccttcagtaa ttacactttc taacatatct gtaacgacat aagctacttc
    15301 aggttctaca actttatgtt cctcaggagc atttttaact aataattgac catcaaaggt
    15361 agttatagta gtaaaaatag aaggttctac ataaataccg tgatttgcca atgtaccata
    15421 agcagcagcc atttgaagtg gagtaatacc attcgccata ccacctagtg tcaatgatgg
    15481 gaattttcta tcacttgctt cagatgaacc tgagtgatta ttctttaaag ttgttatacc
    15541 aaagttcttt aagtaatcca tcattatatc tacacattca tcataagagt ctccaagcat
    15601 ttcagcagtt ttgacagcta tcgtgtttga agatttagca agtgctttac gtaaactcat
    15661 agaaccatga ccagcagtag tagttcttgg attccattta ttattttctt caaacttata
    15721 tcctcctcga gaatcactta gagcagttgc ttgtgttatt tttagagtat caatagctgg
    15781 cgtatatatt gctaaaggtt ttatagaaga acctggctga tgagggttag tagcacgatt
    15841 taaagttttt ctaccactta tatttcttcc tccagctagt gctcttattt caccattttt
    15901 ataatctaag attaccattg cagcttgcgg ttggctaact ccatcaggac cgaccatatg
    15961 tcctgggaaa ttgctatttc tatcaaattc ttcttctaaa gcatcttgca ttttagggtc
    16021 tatagttgtg tttacaatta gaccaccatt gtagaataag ttttgagctt catcatttgt
    16081 atgtccttgt tccattaaag catttataac ttcactttcg ataaggtctt ctacagatga
    16141 agaaacttta tctgatgatt gaggcaattt tatttctatt ttagctttga ttgcttcatc
    16201 atattcactt tgttttatat atttcaattc taacattttc tttaaaacaa tttcttgtct
    16261 ttttttagct tcaggattac ttacagcctt tctaacaact aatgtacctg cttttaattg
    16321 tctataagta tcattagata taagctccca agattttatt ttttcaatca tatcaaagtc
    16381 cacttgagta gggtcatcta aatcatcatc agttgtgtta acaaaaaata ataatttatt
    16441 ctctaaatcc tccttagttt catttccctc taacttagaa gttttatagg ctgaaaatct
    16501 tgatggattt ttagttgaac cagcaagaag tgctgactct gctagagtaa ggtcagctgc
    16561 agacttatca aaataaccac gtgcaccagc ctcagcacct gctagacctc taccaacgaa
    16621 gaagttattt aaataagttt ctagtatttt atctttactt actgttttac tcatctcatg
    16681 tgcatagtaa atatccttta ttttacgagg tatagtttgc tgttcagttg ttaacagcat
    16741 tttggatacc tgcataggaa tagtactacc accttgctta gtacctgtaa gggtctttaa
    16801 tactgaccta aataaacctt taatatcgac acccttatgc tcataaaaac gctcatcttc
    16861 tattgcaatt acagcatgtt gaagataagg ggaaatctta tctaatggaa cgggtgtctt
    16921 tttatttaca cttggagctg tagataatgt ttttccatta gcgtatttaa tagtagttgt
    16981 ttggttcgtc tgtttatcta aaacagcttc tgtaactgga gttacatctc ttagtgacgc
    17041 aaatactaga ccagcagtac ctgctgtacc aacaactagt aaaactaaaa atactatccc
    17101 aaatactctt agttttttaa atttatcgct ttttttagat tttttcttgt tttttgttga
    17161 acgagtcttg ttagctgaag ttctattaat tggtttattt gtattaccag atgagctaac
    17221 tttttttctc ctgattttat ttccattttt atcattatcg ttattcataa gattttaacc
    17281 tccttactta atgcaatatt ataacacaaa taggagtagt tttgtaatta atagaaaaaa
    17341 tgaatttgct ataatattct gtacattgta tatgtataaa cgtaatatat taaaaagtct
    17401 atatttttat tttatttatt tttgacaaat attataaaaa ttatgccaaa gctacatata
    17461 aataaatagg aggggataat tttgaataga atagtagata gaaaaacaaa aaatgaattt
    17521 agaaaaatca tagctatttt tttagtaatt cttttcttgt ctgtatttat aggcagtttt
    17581 atatatatag ataaaacttt aagaccgaca ataacagttt tagcagagac taaagctctt
    17641 gaactagcaa ataggtcgat aaataaagct gttgctgaga tggtagaagg aaaaataaat
    17701 tatgaagatt taatggatat acaacttgat aataatggta aaataactat gatacaagca
    17761 aacactataa tgatgaatga aattgcatct gcaatagctt tagaaataca agatgaatta
    17821 aaaaaggata aaacagcaag ttcttacata cctataggaa cagcattagg aagtcctata
    17881 ttagctaaat acggtcctaa acttgaagta tctattgaac ctataggaac agtatcagta
    17941 aactttaaaa cggaatttga gtcttcaggt ataaatcaaa ctagacatag aatatattta
    18001 gaagctcaaa cacaagttaa agtagtaata ccacttataa cttcaacaaa acaaattaaa
    18061 gctcaaatac ctatttgtga aactataata gtaggtgatg ttccagaaag ttatgtaaat
    18121 attccagaaa aaaatctagg aaatgtttta cctaacactg gaaagaatac caacaagtaa
    18181 tataaagttg tatattttta aataaattca aaataatagt ttataaataa aatttttgga
    18241 aataatatta atataatttt aataaaaatt tatattttat actatgtaaa atatctaata
    18301 tatttggtct gtaggttaaa taaaagtacc tttagaccaa atatattatt aataacttaa
    18361 taacatctta aaacttattt atttatatta tttacaacat ccatttaata aatacaccat
    18421 aacctacagc tgggtcgttt tcaattgcat gtgacactgc tccgattatt ttattatctt
    18481 gaactattgg agtaccactc ataccttgta caataccacc agtttgagct aaaagctttt
    18541 tatctgtaat tttaatttta aaacttttac tttctggatg tttttggttt tctatatcta
    18601 taatttcaat attatatttt ttacacttat taaagttatc ttgtaaaatt atttgagctt
    18661 ttcctaattt aacttcatct aaagatgcta ctttatattt ttttaacttg gaaatatcaa
    18721 agttattaat ttgacctctt attccaaaat cagtattttc tgtaaagcta ccaataatat
    18781 tttctcgttt tgcatttata catccaacac tacctttata agatttttct atattaaggt
    18841 gcttagttgt agaaattgaa cctgttttaa taggtatttt tcttgcatta cccacactta
    18901 ttggatgacc tacagctcca aactctgatg tatctggatt gatatacgtt aaagtagcaa
    18961 atccagaaag taaattgtta aaactaattt tttctaatat gtgtttggta gttttcattg
    19021 ttataagttg tccacctcta tttaataaaa tagatacttg tttttcttct ggtaaagcat
    19081 ttaaggtatt ggcaaagtca ctatatccat caacttcagc attatttacc tttactatag
    19141 catcacccaa tctaaatgga gatgcttctc caggatttct aactatgata ttttttaatt
    19201 ctgcgtcaat ttgaagtacg tttccaattg gtattaaata ttcaggttca tttttttgta
    19261 aattttgtgc ataaattaaa ttatttgaga aaaaatataa taaaaataaa aatgttaaaa
    19321 caattgtaaa ttttgactta gctaagttgg tttttttact taaagtatta tttttaaaat
    19381 gaaaattttt aagttgcata aaacacctct ttcttattaa attattttaa taatagaatt
    19441 gaagttttct tgctgcttct atattatgtt gtgcaagaat catgttttga tgcttggaaa
    19501 actagctcaa taacacctta gttttcataa aaataaataa aataaatagt gtatatgata
    19561 aaataaatac atattcatat aaataaaaat aaacaataac tgtctagcat aagaaaggag
    19621 caaaaaatga aaaaatatag attgttagtt acttgtataa ttgccatggg aatcataatt
    19681 ataggaacat caaatccagt tagcaatttt tacaagacta caaatttgat tagttcagga
    19741 aatatcacaa atgaaaaagc agataaagat gaaaattcaa acaaaaataa taaagataaa
    19801 aatgaagaca aatctaattc taaaagtaat aaagataagt catcagagga caaatcgtca
    19861 gataataaaa atgaaattca aaaaaataaa aaacttttaa tttgtataga cccagggcat
    19921 caaggaaaag gagatagcaa tttagaacca gtagctccag gttcttcctc taaaaaagcg
    19981 agagtatctt caggaacaga gggtattgct acaaaaaaac cagaatatgt tttaaactta
    20041 gaagcatctc ttgttttaaa gagtatatta gaatctaaag gttataatgt aataatgact
    20101 agagagacac atgatgtaaa tataagtaat tcagaaagag caatacttgc taatgataaa
    20161 aaagcagata tggttgtaag aatacatgca gatagtttaa ataattcttc aaaaactgga
    20221 gcatctatat tgattcctga aaaagatgga aaatatacag ctcctatata tgaagaaagt
    20281 aataaatgtg ctgaatttat caaacagaat atggaacaat caggtataca aatcaatgga
    20341 atcgttcaaa gaggcgattt gactggattc aactggtcaa aagtaccagc tgtattagta
    20401 gaaatgggat ttatgagtaa ctataatgaa gaccaaatga tgtcaaatcc agactaccaa
    20461 agaaaaatga tgcagtgtat agctgatgga ttggatgcgt attttaaata agatatgaat
    20521 agagaaaaag ttaggttggt attattttcc taatttttat ctctattttt tattgtacat
    20581 aacacaaaga agaagtttat tttaggttaa attgtcgtaa atataactaa aaatattggg
    20641 aatacttgat ttaagtttaa aaagaatata ttatagtata gaatgtgtaa aatttttttg
    20701 agaggtgttg aaatgctagt agtagaaaat gtgagtcatg gatttggtgc gagaacaata
    20761 ttagaaaatg tatcctttag acttagaaaa ggtgaacata ttgcactagt tggtgctaat
    20821 ggagaaggaa aatcgagttt tttaaatata ataacaaaga agcttatgcc agatgctgga
    20881 aatataaaat ggtcttcaag agctacagta ggttacttag accaacatac ggtattaagc
    20941 aagggaaaaa ctataagaga agtgcttaga gaggctttta agcatatgtt tgacttagag
    21001 caagaaatga ttgctatgta tgacaaaatg ggagaagcta gtgatgatga aatgagtaag
    21061 cttcttgaag aaacagcaga gatacaaact atacttgaaa atagtggttt ttatatgata
    21121 gatgcaaaga tacaagaagt tgcaaatggt cttggtcttg gagaaattgg tcttgataaa
    21181 gatgtaactg acttaagtgg agggcaaaga acaaaagtac ttcttactaa acttctactt
    21241 gaaaatccaa ctattcttat attggatgag cctacaaact atttagacga agagcatata
    21301 acatggctta ctaagtacct tcaagagtat gagaatagtt ttgtcttagt ttctcatgat
    21361 attgaattta taaataatac atgtaatgta atatatcata tggaaaatgg agagttaaat
    21421 agatataaag gaaattatga tgaatttgta agacttaatg atataaagaa acgtcaagaa
    21481 gaacaagctt atgataaaca agttgaagaa agaaaaagac tagaggattt tgtagctaga
    21541 aataaagcta gagtagcaac tagaggaatg gctaacagta gacaaaagca acttgataag
    21601 atggaaatat tagaaagacc aaaagaaaaa ataaaaccta cttttgcttt taaagatgct
    21661 agagcagcta gtaaaataat ttttgaaaca gaaaatttag ttttagggta tgatgaagca
    21721 ttgacaaaac cacttaattt ccatttagaa agaggaaaaa agattgcctt aaaaggaatg
    21781 aatggtattg gaaaatctac cctattaaaa actttacttg gaataataaa accatttgaa
    21841 ggaaatgtta aattaggtga ctatttggaa gttggatatt ttgaacaaga gagttcaaga
    21901 gaaaatagta atacacctat ggatgaagtt tggagtgaat ttccagggct tacaaacttt
    21961 gaagttagac aggctcttgc aaaatgtgga cttacaaatg aacatataac gagtcaaatg
    22021 agagtattaa gtggtggaga agctgctaag gttagacttt gcaaagtaat gttaaaaaat
    22081 ataaatttct tagtattaga tgagcctaca aatcatttag atgttgaggc aaaagatgaa
    22141 ttgaagaagg ctatcaaaga gtttaaggga acagttcttt tagtatgtca tgagccagaa
    22201 ttttattcag aaattgttga tgatgtatgg aatatagaag attttacaac taaaatcgtg
    22261 tagtgattta acttattgct ggggggattt catgaaaaag tttctaaatg tacttttttc
    22321 aatttgtatt agtattgtta taatagtagg tgttattaat tttactgttg gatttaaaca
    22381 actttattat tttgacattg attacttaaa tatatcagag ttatctggtt tatctaaaga
    22441 tgatataaaa ttgaactacg attatctaat tgattataac ttaaataaga atgtgtctga
    22501 gttcaaatta ccaacattaa aatcttctcc acaagggaag atacattttg aagaagtcag
    22561 gaatatattt caaaatataa ataaattagc taaattatta ttggtagttt ctttagtagg
    22621 aattatattg agtgtaaaaa ataaaaatat aaaaatctta aaaactacat caataacact
    22681 tataattatg ccattattat taacagttcc aattttatta aattttgaaa agtcatttat
    22741 tatttttcat aaattattat ttagaaatga ttattggata tttaatcccg atttagaccc
    22801 agttataaat atattgccag aggaattttt ttttcactct ggaatgatga ttttaatatt
    22861 aattttgtta gtaagtatag tactgtttgt aatgtataaa ttatgtaaat ctttatcaat
    22921 aaaaatattt caatgttaaa tagagggata tttttatttc caaaataaga taataaaaaa
    22981 ggattagatt aaaagttata caataaaaat agctgtgcaa taatatttag actatgaaag
    23041 tagttgaaaa atatttatgc acagctattt ataatagaat ttacttttta ctagtgattt
    23101 taactaaatc ttgaaattta ctcataatgt aattgtaatt atctttttta tgaggtaata
    23161 tacaactact gcaatctttg atacctttat ctgtatactt aaagtttcca ccacagtttt
    23221 ctcctaaagc atacaaagga caatagcaaa atagacagtt aaactcttct ggtttattag
    23281 ttttatgaca agggaaaaat tcgcaatctt tgtgattaaa aaacttataa ttttcgccca
    23341 tattttcctc ctaattaaat aaaatttaaa ttttttatac tatataagat tttggtgata
    23401 atataattat actaaataat tttatgaata gtcgataata tagtattatt aatcatagta
    23461 ttaatggtat aattagtcaa cagaaaaata atgtaaaaat aatgtaagtg tattttgtaa
    23521 ttagaatatg ttttataaca aaaaatgagg tgattaagtg aaagaaattc aagaaaaagc
    23581 acttcttgta ggtctgaatt tgactacaat agtgaaaaaa aatgatgata tagacactaa
    23641 tgaatctatg gaagaattaa aagaactaac aaaagctgct ggggcagaag ttgtaggtag
    23701 cttaattcaa aataaacact ctgtagatgc agcttattat ataggaaaag gtaaagtaga
    23761 agaaataaga gcctatagtg actctttaga tgctacttta gtaattttta atgatgaatt
    23821 atctggtgct caaatcagaa atatagagaa tgttgttgga agaaaagtaa tagatagaac
    23881 tactttaatt ttagatatat ttgctcaaag ggcacttagc aaagaaggta aattacaagt
    23941 tgaattagca caattaaagt atagattgcc acgactttat ggaatgggtg gtgagatgag
    24001 tagaacaggt gcagggattg gtactagagg tcctggagaa caaaaactag aaatagataa
    24061 gagacatata cttaataaag cagcagatat aagacgtgaa cttaaagaag taaaaaagaa
    24121 tagagagaca cagagagtta agcgtttaaa gtcaaatata ccgattgtag cacttgttgg
    24181 gtatacaaat gcaggaaaat ctaccctgtt gaacgaatta ataaagacac acaaggatta
    24241 tgaacaagaa aaagaagttt ttgtaaaaga tatgcttttt gcaactttag atgtaacttt
    24301 gagaaaggct cttttgccga ataaaaaaga atttttagta gtagatactg ttggatttgt
    24361 aagtaaactt ccccatgatt tagtagaagc gtttaaggca acattggaag aagtacagta
    24421 tgcagattta atcttacatg taattgatgc aacaaatact agttatgaat tgcaaaaaag
    24481 tacgactgaa ggtgtattaa aagaacttgg tgtaaatgat aaaaaacata tacttgtata
    24541 taataaagtg gacaagctag aattggatat atatcctaaa agtcaagaag atattgtgta
    24601 tatttctgca aaacaaggca taaatatgga taagttatta aatatgatag aaattgcttt
    24661 aatggaaaat acctattcag taaggttgat gttaccttat gagagaggtg atatatttag
    24721 tagaataaaa gataaatata atgtggagaa ttttgagtat ggggaaagtg gtataacgct
    24781 agatgtagat ttagatgaag aagattttaa tatctataga gagtacatac tagaaaaata
    24841 ggttgaatat ggatataaac tgggaaaata tagataatct tgaagattat tttataacct
    24901 atcttcttta taaagaatct aaaacagtat ctcaaattag taaaattaga aatatttcaa
    24961 gtactgaagc aagtgagcaa cttatacaag caaagttaaa aataaaggaa atgcaaaaag
    25021 atgattttga ggcatctaag gatattttgg ataaatttct agaactagat aaaattaaga
    25081 gacttgattt tatggatagc ttagatgacg aaaaaatggt atactttaag agaaaggttt
    25141 tcaaaagaat ccttgtagaa aaaaatgctg aagatttaat tgttttaatt tgggcgacag
    25201 gtgagctaaa agatgatagg tttttgaaat tgcttcatca gctcacaaac cacagacact
    25261 cagatattag aagaataact tattcagcaa tcagaaagat tgaatcacca agtagtagag
    25321 aagtactgca aaaaggtctc tacgacaaaa atgcacagac taggcagtac tgtgcaaaag
    25381 ctctttcaaa attgggagat gaaaacagtt taaagatttt acagcagttg aaagacaaga
    25441 ataagaattt tgaaaaagaa tatgtgctta gagcttatga cgaagccatt aagtcattgg
    25501 aaaatgcaaa aaatttgaaa aagtaatttt ttattaaagc aatttgttta cttaatgatg
    25561 tatttctaaa gtttaacaag gtaataaata aattaatggg aggggtttca aatgatagta
    25621 agacgttgtg caggtggagt agtgttttat gctaataaag tacttattgt aaaaaatgat
    25681 aggggtgaat ggacacttcc aaaaggaaag atactaggag ggggacttcc atatgagagt
    25741 gctgttcaaa gagttaaagt agaaactggc atagatgcta aaatgataga tgtggctggc
    25801 gatactatgt atgaattttt ctcaagaagt agacaacaag aagtttgtaa tgcaataatg
    25861 tggtatgtta tggaagcttg taacactgat tgtgtacttg ctcctgaatt tcaagaaggt
    25921 ggattttata aagtaaagga tgctttagaa atgttatcac atcataaaga gcaagcttta
    25981 gtagaagtat cttataaaaa gtttaaggaa ttaaagaaat tagtttcaga gggcgatgat
    26041 gttccagcaa attaattgtg aatttagagg attctgagga agggatagcc ccttcctttt
    26101 ttaattgaag cagttttaag cgtaaattat atagtctctt taaactatga tttatcaatt
    26161 tagagattta gtagtttata tttaaaatca gtaagatatg tttaaaataa aaattaaaaa
    26221 gtatacaatt ttattatata attgtcttat gatgttgatt aaagaattat taaaagtcca
    26281 tttattttta aatagaataa ttttataaat atatatttga tgtgtatatt agaaaatgat
    26341 taaaatagga ggaaatacaa tgagcaatta caggacatta catgaatttg gaacggatga
    26401 aataacgatt gaaaagtctg tttttattgg atatgctaag cctataaaga gtgaagaaga
    26461 agcattagag tttataaatg aaataaagaa aaagcataaa gatgcaactc acaatgtgtg
    26521 ggcttatact gtaggtaaaa atatgaatat acaaagatat agtgatgatg gagaaccaca
    26581 aggaacagct gggataccta cattagaggt tataaaaaaa gaagatttga gagatgtagc
    26641 ggttgttgtt actaggtatt ttggaggaac aaaattaggt gcaggtggtc tagttagagc
    26701 ttatacaaaa ggtgctaaac tgggacttga agcaggtaag ataatttata aggtaatgta
    26761 tcaagaagtt aaggttaaga tagattatac tcaacttgga aaagtgcaaa atgagttaat
    26821 gaatttgggg tattttataa aagatactgt atatgaggat aatgttgaaa tagtagtata
    26881 ttcaagatta gaagatgtag agaaattatc agaaaaaatg attgatataa caagtggaac
    26941 aggaaaaata gttttaggag aggaatttta tttgtctgaa caaaatggag aaattttaat
    27001 ataatctaaa aggttattag aatagttaat acctcaaata gccaattagt atttttaagt
    27061 gaaaagccat taaattgatt taatctaaat caatttaatg gctttttatc ggattttgca
    27121 cagaatcctt tttttacttc aaataaaccg actaaaagta aaaatatccc aaatattttt
    27181 tttaatatat ctgaagacag ataattagct aatagtgaac caaatacagc tcctaaaacc
    27241 ccaaacacag ctataggtat aaccaattta aaaattacat ttttattttt tatatgtata
    27301 gcaagtgcta taagtgtcat agggatagat gaaagtaaat ttacgctttg tgctatttta
    27361 gtgtctatgc cagcaagtaa aacgagtgca ggtataagga ttgttcctcc acccattccc
    27421 attcctccaa taattcctgc aaaaaatcca attagagcaa ataacattaa aacaccatcc
    27481 ttaatgctgc aattatcata attattccaa aagaaattct aagaaacttt ccagtaacct
    27541 tatttagtaa tttagctcct ataataccac caagaatact tccaactgca actttaattg
    27601 ttaagttaac atcataagta cctttagata catataaaac agaacttgct gtcgttaaga
    27661 atattattat agaaagagca gttgcatgag atttatgttc ttctacctta acgatgtcat
    27721 ttaatattgg aactaaaaga gtacctcctc cagaaccaaa aactccattt ataaatcctg
    27781 taaaaactcc aattatcgta ttttttaagt taaaatcaaa gtattttttc tttaaatcat
    27841 cagttttttt cataaaaatc accttctgaa atagttttac caaaataggc aaattatatt
    27901 tatataaaat aagattaaat ataatctttc atatttgttt aattttgcat aaaaaaaaac
    27961 atattaaaac aacatattaa tatatggtat aatatattaa aacaaagtag caaatttgag
    28021 aggagataac aatgtcaaat ataaaaatac aaatagacaa aactgtagaa tggcttataa
    28081 ataaggttaa tgaggcaaat gcaaaaggat taattgttgg tgtatctgga ggaattgatt
    28141 cggcagtagt tgctaattta attaaaaagg catttccaga aaactctatg ggagttataa
    28201 tgagcataaa aagtaatcct caagatagag aagatgcact taaggttata gagggatgtg
    28261 atatagaata ccttgattta gaccttatag aaccacaaag tgctatatta gacatggtag
    28321 taggaaattt aaaagataaa catttatata gagaagaata tttaaagatg acagatgcaa
    28381 acttaagagc aagagtaaga atgagtacaa tttatacaat tgctaacaat ctaggatatc
    28441 tggttgttgg tacagacaat gctgcagaaa tacacacagg atattttact aaatttggtg
    28501 atggtggagt tgatatttta cctattgcaa atttaactaa aggagaggtg tatgaatggg
    28561 caaaagagct tggtgttcat gaagatttaa taaacaaagc tccttcagca ggtctttggg
    28621 aaggtcaaac agatgaagat gaaatgggaa ctacttacaa tatgatagat gctgtgttag
    28681 aaggtagatt ggatgaagtt cctaaaagag accaagagat aattgaaaga cttcatagat
    28741 taagtgaaca caagagaaaa acgcctgcac aacctcctaa gttctaattt actatttata
    28801 agatttgtga taaaatttac aagtgatgaa aattagaata attaattgta cgtattaagg
    28861 agtggaaata tgggacgtat aggtaatata attaatagaa aaggtaaaca agatgctcaa
    28921 agagcaaaga tatttactaa acatgctaga gcaatagcag ttgcagctaa agaaggtgga
    28981 gcagatccag agtacaatgc agcgttaaaa acagctatag aaaaagctaa ggcagataat
    29041 atgccaaatg ataatataga tagagctatc gctaaaggtg ctggagcagg tgctggagaa
    29101 gactatgaaa ctatagttta tgaaggatat ggacctggtg gagttgctgt tatagtagaa
    29161 actttaactg acaacaaaaa cagaactgct ggtaacgtaa gatattattt tgacaaaaac
    29221 ggaggaaact taggaactag tggatgtgtg tcttttatgt ttgataaaaa aggacaaata
    29281 ttagttggtt taggagatgg agtttcagaa gaagaattga tggatgttgc attagaagca
    29341 ggagctgaag atttcataac agaagaagat ggttatgaaa taataactac accagaagat
    29401 ttttctagtg ttcgtgatga gttaaaagct aaaggatatg agtttatatc tgctgatgtg
    29461 aagatgatac ctcaaactac aactgtattg actgaagaat ctcatttaaa aatgatgaat
    29521 aaattagttg atatgcttga agaagatgat gatgttcaag atatatatca taactgggaa
    29581 gtagaataaa tagtataagc atattgaaat ttataagtat tgaatcaatt ttaacatgtg
    29641 ataaatttta tagaataaga aacactaaaa tagacatacg cacctctaac ctatatttgc
    29701 ataataatat gtaagcatag gtaaggggtg tttttatatt tataggttaa gaaatttaaa
    29761 gtaaattttg agctggactc gattgtctat aaataattga tgggtatata acttgttgct
    29821 gaataccatt tatacgccca gttactgtat caataatcat cctaagacta cgttctgctg
    29881 tctcttccat ttttaaatct ataactgtca aaggaggaat acttcttgcc attagtcgtg
    29941 aaggtccatt acttgctgca aatatttgaa tatcctctcc aattacaatt cccgattcat
    30001 gaaatgcatt catcatacca agagctacca catcatgatt acaaaaaatt acttttttta
    30061 gtgaattttt tgcaagcatt ttttgagcaa tatcatatcc actatctatt ttattagaac
    30121 aataatatat agaatctgat agatgtattc catgttcttt acatgttttt aaaaaagatt
    30181 taccccttaa agtcatacat gtaaaggaag ttgggttgaa tacaacagca atatcatttt
    30241 taccattttg aatagcatgt aaagctgcta tacgaccagt ttcctcatta tcaatagata
    30301 cacttgaaaa accaggtatt tcacgattta caacaactac aggaatttta ggtttatttt
    30361 ttactagata ttctatatca tcagcattga tacctgttaa aacaattcca tcataataat
    30421 tatttgcaaa aaagtattca tcatcacata gatgaccaac tttgtaaggt cgtatgatga
    30481 tatttatgtt taaatgttct aaagaaatta tgtggtttat tgcttgaagt atattggttg
    30541 atgccaactc aaatccctca tcctgccaat aaaaagctat tttaatatta ttatgtttaa
    30601 taggagtaga gcgtttatat ccaagcttat cagctgaatt taatataagc tcttgtgtat
    30661 gtagcggaat cctgcgttca tttcctttac cacttaagac gtacgagaca gttgctgaag
    30721 aaagaccaca gtcttttgca atatctttga ttgttgccat gataaaatcc tccttaaata
    30781 gttgcttaaa tttgcttata ccaataatct taacatatat tcaactaaag aacaatattc
    30841 tacaagttgc atattttaca ggattagaat aaataaattt aaaaaaactt cttacttatg
    30901 ttgacataat ttagtttata ggattataat atgcttaaat aagcttataa ttaacttaaa
    30961 ttaagcaaag ttaagcaggg acatgcaatg ctagcttgca atgtgactgt aaagggggaa
    31021 aaggtatgag taaggtaaat atatttgaag ttggtcctag agatggattt caaaacctga
    31081 aagagttttt agaaactgat aaaaagctta agattattga tggtcttatt gcttcaggga
    31141 ttaaacaact tgaaattagt tcatttgtaa gcccaaaagc aatacctcag atgaaagatg
    31201 caaaagaagt agcaacttat tgtgtagaaa aatatcctga tgtgaaactt tatgcattag
    31261 ttccaaattt atacggtgct aaagttgcat gggagtgtgg tataagagat atatcctatg
    31321 ttatatcttt gagtgaaact cataataaaa aaaatataaa tcgtacccat gaagagtcta
    31381 ttgatgagct taaaaaaata atgaatactt atccagacat gaatatatgt attggaatgg
    31441 ctactgtatt tggatgtcct tttgaaggtt taccaaaaat tgaagatgcc ataagatttg
    31501 ctaaatgcct ttgggaaaac ggtatcaggg agataaatct agcagataca atagggattg
    31561 ctgaccctaa gcaagtaaga gaaactatta gtgcattagt tgaagcttta ccagagtgca
    31621 gatggcaagt acatattcat gatactagaa atatgggaat ggttaatact cttgtggcta
    31681 ttgaatctgg aattactact gttcagtcta cacttggagg attaggagga tgcccatttg
    31741 ctcctggagc atctggcaat actgcaactg aagatttagt atatatgctt gaacgtatgg
    31801 ggtatgatac aggagttgat tttgttaaac ttcttgaatt atcaaagtat cagaagagtt
    31861 ttgtaaatgg agtatatagt ggtcatcatg taaatataaa gtctgatagt tgtaattgtt
    31921 agttctattg attaatcgat taaaaaatat gttgattaaa aaaatatgct gattggtaaa
    31981 atagatttat tgataaaaaa tataaattat aaaatacatt atctaataaa acaaataaaa
    32041 aaattgaggg ggattagtat ggatattata gctttacttt tatttttagg agttattatt
    32101 attgcatttg tacgtaagaa taatgcaggt attttggctc tagctattgg tgtcatagct
    32161 gttagaattt ttggaatgga tgataaagaa ctttatggag ctataagcaa ctcaatgttt
    32221 actacacttg ttggtataac actactcttt gcaatagtga actcaacagg agcattaaag
    32281 cttgttgcag aaaaaattgt agctgcttct ggtaaacgtg tttggcttat acctatagca
    32341 gtttttatag caggatttgt tgttgctggt gttggacctg gggcaattcc agcacttgca
    32401 attatcccag caatggctgt tcctgtagcc ataacagttg gttataaccc tgtaatgctt
    32461 gcacttatag gtgaatgtgg attaatggca ggtcgtatga ctcctattac tccagaaggt
    32521 caaattataa caaccgttgc agaaggggtt ggaattgaaa atgttatgcc tactatctta
    32581 gcctgtcaaa ctctttctac aattgtattt tcaatagtat tgtttataat tttcaaagga
    32641 tataaactta aaaaacctat aaatgttata gatagtaagc aaatagaaaa attcaatata
    32701 aagcagataa tttctctaat aagcatagta gttatgattg cttttatcat aatctttgat
    32761 atgaatgttg ggcttgcagc ttttgcagtt tcagctgtac ttattttttt caatattgct
    32821 gatgaaggag aatgtatcaa atcaattcca tggagcacga tagtaatggt tcttggagta
    32881 ggtgcaatga tgacaatcgt agatgcagca ggaggtattg acttgatgag taatgcatta
    32941 tcatcactga tgaatagtaa aacagctact ccaattatga gtatttcagc aggacttatg
    33001 tctatggtta gttctgcttt agcagtagtt tatccaacta tgatgcctat gtgtgttgat
    33061 atagctaagg cagttggagg agtagaccca ctagcactta ttgctgcagt tggtgttgga
    33121 ggttctcttg ctggtgtaag cccactttct actggtgggg cattgatttt agcagctatg
    33181 ggttcaagta agaaagactt taataaggag gaacaaaata aggtttttgt acagcttttc
    33241 attatgagtg cagttggctt attggttatt gcagtagtat caatattatc atttaatgca
    33301 ataacacatt taatgaacta attagaataa ctgaaaataa atcattaaat ttaactttta
    33361 atattaatca atataatata atttggaggg gaagttatga aaaaaggagc attaagcaat
    33421 ataaaggtac ttgaccttac aagagttctt gcaggtccat attgtacaat gatgttggcg
    33481 gattttggag ctgatgttat aaaaatagag atgccaagaa aaggtgatga tacaagaggt
    33541 tttggacctt ttgtaaatgg agagagttta tactatgcaa atataaatag aaataaaaaa
    33601 ggtatcactc taaaccttaa atcagaagaa ggaaaaaata tatttttaga aatggtaaaa
    33661 catgctgatg ttgtagtaga aaattacaga ccaggtgtaa tggaaaagtt aggtctgggt
    33721 tatgatgagt tggctaaagt taacaatcaa ataatttatg gagctgtatc aggatttgga
    33781 tgttatggac catattcaga acgtccaggt tatgatatta ttgcacaggc aatgggtggt
    33841 cttatgagta taacagggca agaaggagga gaaccaacga gagttggtaa tgctatgggt
    33901 gatgtactag gtggaatgaa tttaactatt ggtatactca cagcactcaa cgctagaaaa
    33961 ataataggaa aaggacaaag agtagatgtt tccctcgttg attctgttgt atcgtctctc
    34021 gaaactggta tacagagata ctttgattca ggacaacctg aacttatggg taatagatat
    34081 gcttcagcct atccttatga ctcttttaag gcaagtgatg gaagttttgt aataggctgt
    34141 ggtaatgaca aactttttga aaagttatgt aaaaaagttc tttttagaga agacctttta
    34201 atagatgaga gatttaaaac aaatcttaat cgttgtgaac attacaaggc tcttaaacca
    34261 gaaatagaaa aatggtctgt taagcataca ataaaagaag cagttgactt aatatcagag
    34321 gctggagttc ctgctgctcc tatctataat cttgattgtg ttacaacaga taaacatata
    34381 gcaaaagcaa gagaaatgat tgtagatatt agtcatccta ttataggaaa tatgaaagtc
    34441 aatggaaatc cagttaagtt gatgacaaca aaagctaaaa ttgatatgcc agcaccatct
    34501 ctaggggaaa gtaacgagga gatttataat ggttggcttg gttttgatag tgataaaata
    34561 aaagatttaa aagaaaatgg agttatttga ttaaaaaata gctaaacaca cttgtgactt
    34621 tagtcatggg aggttcagtt agaataatat caaaaaatat taaatcaata ctgtaaatca
    34681 aatatagaat tgtcagaagc atttaagatg ggaaaagcta cttagacatt gagtctttgt
    34741 ggcttttcct attttcatta agaattttat attatcagaa tagatatgta ctaaactaaa
    34801 gtattaacaa tttaaattaa tagaaaataa actacctaat tatgttgaat aaagactact
    34861 ttagtgaaaa caaacttata gagatgctat ttaaattatt ttaatggaaa tatcaaaaat
    34921 gagactgcat tctcaaaaat gagactgcgt tttttaagta ttttctcgaa aatgagacaa
    34981 aaatattgtt ttatgggttt tattgaagaa aaattcttct gtgaattaca atttaatcaa
    35041 ttttaaagtt gaaaattatt aaaaatttag tgaaatttat gtatatttcg tttattttat
    35101 taaaaataac aattttttac taaaaataaa gaacttcaat ggtgattggc atagtacttg
    35161 ctattttata ttaacaagaa atgtagaaaa aataacaatg tataatgggg ggaatatgat
    35221 ggaatctgta atggaaaatt tgaacaatct taaggtggag gttaaggaca aagtctgtgt
    35281 tattactata aatagaccta aagctttaaa tgctcttaat agtgacactc ttagggaact
    35341 atcacaagtt attgatgtag tatcagaaaa tgaggcaata ttaggagtta ttattactgg
    35401 agaaggtaaa gtttttgtag ccggtgctga tatacgccag atgcaaaact ataaaagtga
    35461 ggaaggcaga aaatatgcag gttatgctca gggaattttt gataaaattg aagctcttga
    35521 gaaaacagta atagcagcag ttaatggata tgcattaggt ggtggatgtg aacttgctat
    35581 gagttgtgat attagaattg catcagaaaa ggctatattt ggacaacctg aagttaattt
    35641 aggagttatt ccatgttttg gtggaactca aagattatca agacttgttg gaactggaat
    35701 agctaaagag ttaattttta cggggagaca agtaaatgca gaagaagcta aatctattgg
    35761 acttattaat aaagtggttc caagtgattt acttttagaa gagtctatga agatgatgaa
    35821 ccaaatagtt gaaaaagcgc caatagcaat aagatatgca aaagttgtta ttaacaaagg
    35881 gatagatatg gatttaaaga atgctcttga actagagaaa gatgttgcag gtttaacttt
    35941 tgctacaaga gataagcaag aaggaatgaa tgcatttata gaaaagagaa agccagtttt
    36001 tgaaaataaa tagctattat aactaaaatt ggaacaatgt agaatatatt caaaatctaa
    36061 atttcgtgaa aaaatacata tagtttaatt ttaaaaggag ttaatttatg aatacgggag
    36121 taattataag tttagtaggg attatcatag ctctatctct tctagttgta atggttatga
    36181 agggaataaa tatttttatt atagcaattg tatgttcatc attagttgct ataacaggaa
    36241 gcctaaatgt gtatgatgca cttaaagtgg attatatgac agggtttgtt ggatttcttc
    36301 aggcaaattt ctttattttc ctaacaggga cattaatggg aaaaataatg gagattactg
    36361 gtggagcaaa atctattgct aagatgattg taagatggat tgggaaggat aaagcactac
    36421 tttctattcc aatagcttgt ggaatattag cctatggtgg agtaagtgtt ttcgttgtaa
    36481 gttttgcagt gtttccgata gctttagaaa tttttaaaga agctgatttg ccacgaagat
    36541 ttataccagc agctttgact tttggatgct caacttttgc tatggttgca ccaggggcac
    36601 cacaaattca gaatattgtt ccagcatcaa cattagggac agatattatg gctggagcag
    36661 taaatggttt tatatcatgt gcagtaatgt ttatagttgg aagtacaatt ttgtacaaaa
    36721 tggtttcaaa agaaaaagcc aatggtggtc attttatagc acatgaatca gatatatttg
    36781 atgaagcaga agcaactaat tctaagagag aaaatggtcc caatggtgta attgccttaa
    36841 ttccattgat agtgtcgatt ttaataatta atgtaaaagt taatggaaaa gcgatagtac
    36901 caattgaggt gggagtattt attggagctt tattagtata tttattgtta aataaatatc
    36961 aagataatac taaaatagtt ggacatgttg gtgatgcatg taaaactaca gtagtagcaa
    37021 tatgtaatac ttgtgctgta gttgcatttg gttcagtagt aaaatcagca gttggttttg
    37081 acttcatagt taatgctatg actaatattc ctggtcctcc tattgcaggg gctgcagttg
    37141 gaactacagt tattgcaggt atttgtgggt cagcttcagg tggactagga attgcagctc
    37201 cacttcttgg accagtattt ttagccaagg gtgtttccgc tgcagcatta actaggacta
    37261 tggcaatttc atcagcagca ttagattcac ttccacacaa tggatatatt gttactgtaa
    37321 caaatggtct ttgtaaagaa acacataagg atgcctatat gccaatattc tgggtaactg
    37381 ttttaacccc atttattgga accatagtag cagtcctact gtttacaatg ttcccgggat
    37441 tgccatagga gaaaagataa aaggtataaa ttatattttg atattataaa atcaattttc
    37501 aaaatatata caataaattt catgttttaa tactgtaaat atgtatttaa aataacaaat
    37561 tgaccatata aggaggaatt gaaagtgtta aataaaccat tagagggagt taaggttatt
    37621 gacctaacgt attttgtagc aggtccaggg acttctaaaa tattagctga ctggggtgct
    37681 gacgttatta aggtagagcc aagctttgga gacccaggaa gaaagacagg ggcaacaatg
    37741 acaatgccaa tagatgatta taataatcct ttttacagca catataattc aaataaaaga
    37801 ggtttgtcta taaatcttaa gagtgaaact ggaattgaaa ttatggataa gcttttaagc
    37861 gaagcaaacg tatttgtttc aagttataga acaggagcat taaaaagact aggtcttgat
    37921 tatgaatcat taagtaaaaa gcatccacac cttatctggg gtcagataaa tggttttggc
    37981 gattttggac ctgctaagga taatgcagga tttgatacag ttgccttttg ggcaagaagt
    38041 ggggctatgc ttgatatagc agaaaaagat acaagtccaa taaatccacc tatagccttt
    38101 ggagatgcaa caacttcttg tagtttatct ggaggaatat gtgcagcttt gtaccagcaa
    38161 gctaagactg gaaaaggaca aaaagtaatg gcatctttat ttgggcaagc aatatggaat
    38221 gcatcatctt tattagcatc tacacagttt agtgatgagt atccaaaaac tagaaaaaat
    38281 gctatttcac cagtaataaa ttcttatcgt tgtaaggatg gaaagtggat attcttaagt
    38341 attttagagc atgaaagata ttttgaggca ctttgtagat tatttggaag agaagactta
    38401 ataaaagaag agaaatttgc atcttcagta gaaagtaaga aaaatgctac agaacttatt
    38461 aatattttag atggtgaatt tgcaaagttt actcaaaatg agatggttga aaaacttact
    38521 gctgctgata tagctcatga aaaaattcaa catgttaatg atgtagtgac tgatgaacag
    38581 gctattgcta ataactatat atatgaacat acaagtaaag atggaaataa gacaatgctt
    38641 gcatctactc cagttaaatt tggaaatatt gaagttaaca tgacttgtga tgctccactt
    38701 ataggagaac attcagatga gatattgaga gaacttggat acagtgattc tgatatagag
    38761 actctaattg aatctggaat tgtaacaatg aataaagaag catgtgaaat atgttaataa
    38821 acatgtgaag tatgttaata aataggaggg aattttatgg attttagatt aacagaagca
    38881 caattaatgc ttcaaagagt agcaaaagaa tttgcagaga atgaaatagc accaatagct
    38941 gctgaaacag ataaaacagg tatttttcca agggagcttt ttagcaaaat ggctaaaata
    39001 ggatttaatg gaattggaac acctgtagag tatagtggtt ctggtggggc agatatagaa
    39061 aaggtaattg ttgttactga gattgccaaa aaatgtgcag catctgcagc aatattatca
    39121 atacacacaa tatatgctca agctattttg aaatttggta ctgaggaaca aaagaaaaaa
    39181 taccttccaa tgatggctga aggtgggtgt gtaggtgctt ttgccttaac cgaacctaat
    39241 gctggttcag atgctgcgag agctgcaaca actgcaatta ttgatgaaga aactgatgaa
    39301 tatgttttaa atggaactaa gtgttttatt tctggtggag gtcaagcaga atcattaata
    39361 atatttgcat tgacagaccc atcaaaggga ataaagggaa tgtctgctat cattgtcgac
    39421 aaaggtactc caggtttttc aataggaaaa attgaagaaa aaatgggtat tcatggttca
    39481 gaaactgcag agttaatatt tgataactgt agagttccaa agtctaactt attaggaaaa
    39541 gaaggtaaag gatttaatat agctatgact tgtttagatg gagcaagaat tggagttgga
    39601 gctcaagctg taggaattgc tgaaggggct ttagaagaaa gtatcaaata ttctaaagaa
    39661 agagtccagt ttggcaagcc aatttcagct ttgcaaggta ttcaatggta tatagcagat
    39721 atggcgacaa tggtggaatc agcaaaatta ttagtatatt atgctgcaga tttgaaagct
    39781 agaggagaaa aacacactaa agaagctgct atggcaaaat acaatgcgtc acgtactgca
    39841 agagaggtta caaatttagc ccttcaaatc catggtggat atggatatat gaaggattat
    39901 ccattagaga gaatgtacag agatgccaaa ataacagaaa tttacgaagg tacttcagaa
    39961 atacacaaag ttgttatttc aagagcagta ctaggatagg aggaaaagaa tgaatatagt
    40021 tgtttgctta aagcaagttc cagatactaa tgaagttaaa ataaataaag agacaggtac
    40081 tctaataaga gatggtgtac caagtataat caatccagat gatagaaatg ctttagaaga
    40141 agctctaaaa atgaaagatg aactaggagc agtgattaag gttataagca tgggacctcc
    40201 tcaagctaaa agtgctttaa aagaagcttt ggcaatggga gctgatgaag cttatttaat
    40261 ctcagataga gcttttggtg gttcagatac ttgggctaca tctacaatta ttgctgctgc
    40321 tatagagaag gttggaaagt atgatgtaat attctgcgga agacaagcta tagatggaga
    40381 tacagctcag gttggaccag aggttgcaga atttttaggg ataccacaag ttacttatgc
    40441 gaaagaagta aaggttcaag atgacaaatt actagtaact cgttatacag agacagggga
    40501 ttatttaata gaagcaaaga tgccagtttt attaactgct ataaaagagt taaacaatcc
    40561 aagatatcca agtgtcaagg gaatcttaga agcttataat aatggagatg caaagattac
    40621 tgtattaaca cttgcagatt tagatgttga tacaactcaa attggattaa agggttcacc
    40681 tacaaacgtt tataagtctt ttgtaccagt taaagacaag cataatgaaa taatagaagg
    40741 tataaataaa aaagaaaaag ctgagaaact aatagaaata ctattcgatt tgaaactagt
    40801 ataggagtgt taagatgatg agagcaaaag ttaatcaagg tataaattta aatgattata
    40861 atggtgtttg ggtaatagga gagcaaaggg aaggtaaaat taatcctgtt acgatagaac
    40921 ttattggtga aggaagaaag cttgctgacc aattaggaaa agagcttgca gttgtgatag
    40981 ctggatatga agtagagaaa gaagtgaagg aattacttca ctatagtgtt gataaagtat
    41041 attacatcaa tgaccctctt cttaaagatt tcactactga tggatatgca atttcaattg
    41101 caaatttaat cgaaagaaaa aagccagagg ttgtattggt tggtgctact tctataggaa
    41161 gagatatagc gcctagaatt gcaggtaagg ttggaacagg tctaactgct gactgtacaa
    41221 aacttgaaat agattcaaca gataataagt tattacaaac aagaccagca tttggtggaa
    41281 atttaatggc cactattgtt tgtccaaaaa atagaccaca gatgtcaaca gttcgtccag
    41341 gagttatggc aaaagctgta agaaacgaat cagagactgg aattttagaa gttgttactc
    41401 cagaacttac tgaaaaaatg attagaacta gattagtaga gatacttcca caagaaaaga
    41461 aatctgtaaa tctaactgat gctagaataa tagtatcagg gggaagagga ttaaaaagag
    41521 cagaaggatt tgagcttata aaagaattgg ctgataaatt gggggcagag attggtgctt
    41581 caagagcagc agtagattca gggtggatag aacattctca tcaagtggga caaacaggaa
    41641 ctactgttag accagaacta tacattgcat gtggaatatc tggagcaata caacatttag
    41701 caggtatgag tgattctaaa tatatagttg ctataaataa agatgcaaaa gcccctattt
    41761 ttagtatatg tgactatgga atagtaggag atttatatga aattatacct gaaatgatag
    41821 aatcattaaa taggtaactt ttagaaaaca ttgaatatgt agaatgataa gtcttataat
    41881 attgtattgt aagacttatt atttttgctg aaggttatag tggggagatg aaacaatgaa
    41941 agagcagtgg tataaggata tatttgcaag agtattatct atgactgatg atggttttat
    42001 tgttgtaaat acaagtggag taataataga tattaatgat aaatattgtg actttttagg
    42061 aaaagaaaga aaagatataa taggacaaaa tatacaaagt attataccaa atacaaaaat
    42121 gctagatgtt atgaaaaata agtattgtga agaaggagct atacatcatt attcaggtgg
    42181 aaatacaaaa gaaaaaagtg taatagtaag taggtcttat gtggaaaatg acaatggaga
    42241 agtagttgca ggagtagctc aagttaaatt tagattgcaa tcatttgatg tagctaaaaa
    42301 gttaatgtca gaatatatgg agctccaata ttataaagag cagtttaagg ataactgtgg
    42361 ttttgacaag ctcataggtg aaaatagaga cttcatagaa cttaagaaaa caggtgtgaa
    42421 agcttctaaa acaaactttc cagtactttt aacgggtgaa acaggcacag gtaaagaagt
    42481 ttttgctaga gcaattcaca ataatagcag taggtcagat aaacctatgg ttagtataaa
    42541 ttgcgctgca ataccagaag aattattaga gtcagagcta tttggctatg acgaaggagc
    42601 ttttacagga gcaaaaaagg gtggtaaaaa aggtaaattt ttagttgcga ataatggtac
    42661 aatcttttta gacgagatag gtgacatgcc tttgaccatg caagcaaaac ttttaagagt
    42721 tcttcaggaa agtgagatag aaccagtggg aggtcttaaa actataaaga tagatgtaag
    42781 agtaatctct gccacaagaa agaatttaag taaaatggtt gaagagggat tatttagaga
    42841 agacctatac tatagattaa atgtaataaa cattcatatg atggaattga aggacaggca
    42901 agatgatatt ttattgctag caaattatat attaaacaaa ttaaatgttg aatacaagga
    42961 attgaaggtt ttaagtgata aggttaaaaa ttgttttatt aactatactt ggccgggaaa
    43021 tataagagaa ctacaaaatg taataaaaag tgcctatgct gtaagtgatg atatggtcat
    43081 tatgatgtgt gaccttccat ctaagatgga taatatcagc agagtagctc aatgtaatgt
    43141 ggatagcaat tgttcaattc atgaaatggt tgagaattat gaaaagagct taattattga
    43201 tgtgttaagg aagtataatt ggaaatgttc taaggcagca gaggttatgg gaatacacaa
    43261 gagtctttta tataagaaaa taaaaaagta tgaaattgaa ttaaataatt aagataactt
    43321 gaattaaggc aatgctaaaa aagcgccctt attcaaagcg aactgtataa actgttttat
    43381 gcagttaaaa cgtgtaaaag tagatgtgaa gtactaatgt caatcattag tactttttta
    43441 ttatatttat aattactatc tatatttcag tatcttttga tatctaagat tattatgttt
    43501 aatttagtat ataacaaaac cctcttagtt atggacagtt ttttatattt taactgtcta
    43561 ctaagagggg atagtttagt ttaaatttag agtaaatttt atctagcatt tataaaaaat
    43621 attttgaata gcttttttgt tatttagtca taattttcta cttctttatc aagttctttg
    43681 ccacaagaat cacacttata tatagcattg tatccgtaat catattcatc aactattttc
    43741 ttttgtctaa atacaccacc acactcacat aggaaaccaa tttttataag tgtaacgata
    43801 aattgtgatt ttataaattt tatatcattg catttatcgt tagtgtaaaa tgtataaata
    43861 taagagttat cgcactcatc tcttaattca aatctttcaa tatatgtgtt attttttaat
    43921 tcctcaaatt cttctttagt taaatatgat ttttgttgca attcatgtaa acgcttaatt
    43981 tggtccatta tttatatcct cccaataatt ttagtatttt aagcttagat aaggaatcta
    44041 agttttatta aatttatccc aaggaattat ttaaatcata tttagtttta ttaattacaa
    44101 catatataaa ttacacgaaa ataaaactaa ttgtaaaaat aatatcctgt gaaaacctaa
    44161 actactaacc ccttactaac ccgaaaacta aaaaaagaaa atatatccaa atataagtga
    44221 tatataaata aagatgtttc atataaattt aactttaaat attctgacaa taatatatat
    44281 aaatatatta tagttaaaat ataccacata ttttgggtat tgtatactgg aattagagta
    44341 attaatttat tttttaacat acaaatgatt ttatgaaaaa aaacaagact caaaaatttt
    44401 tatattcttt gatagaataa aattgagagt aaatttattg aattttataa aatttataag
    44461 gggtgagtct aagatatgca tgaagataaa aatgatatat ttgaatcaat agatactgag
    44521 gattttgata ataaaacaga agaagaatgt ttagagctag taagaaaaaa tggattgaat
    44581 ttaatataca taaaaaatca aacagaagag atatgtctag aggctgttag acaaaattgt
    44641 aatgcaataa aatgtgttca gaatcagaca gaaaaaatat gtatagaagc agttaaacaa
    44701 gattggagaa tgttagaatt tgtggaagag ccaacagaag aaatttgcat gttggctatt
    44761 aaccaagatg gaacagcatt aaaatatata gaaaatcaga cagaggagtt atgcttaaga
    44821 gccgttgaaa aaaatggtgc agctttggag tatgtaaaag aacaaacaga agaaatatgt
    44881 atagaagcag ttagaaacag cgaatttgga ttggcaagag tgaaaaatca aacagaaaaa
    44941 atatgtatgg aagcagttaa gcattgtagc tataatctaa aatgggtaga aaatcaaaca
    45001 gaagaaatat gtatggaagc agtgagacaa aatgggcttg atttgaaatt tgtaaaaaat
    45061 caaacagaaa caatctgttt aagagcagta agacaaaatg gaatggcatt agaatttgta
    45121 aaggaacaga ctgttggtat atgcttaaag gcagtaagac aatatggaat ggcattaaaa
    45181 tttgtaaagg aacaaacaga agaaatttgt acagaagcta taaaacaaga taaaagagca
    45241 cttagttttg taaaaggaga taaggaaaaa tataaggctc tatatgataa taatgaacca
    45301 tttgcaaaga gatatgttag aaatgtaata gaaaaagaaa acagagcatt gattaaaaaa
    45361 ggagaagaaa atgcaaaaat caaaattgca ggaaaacttt atgatagagg catgtctttt
    45421 gaagatatat cagatatagt tgagattgaa ataagcaagt taaaagtgag tttaggtgtt
    45481 ttttaaagta ataaatgtag gtgtttttta tgggaaatag ttaataaaaa aataatcgac
    45541 tagctctttg taggacagca aaattagtga tataatttga tgtataataa attattttta
    45601 ttagataagg gggaattaaa gttgaagatt acagtattat accaaacaag aagtggaaac
    45661 acagaaaggg tcgctaagct gatagaagag ggcgctaaaa aggtggatgg agtagatgta
    45721 aagcttatga gacttgataa tattgattta gattatttaa atgaaagtaa agcagtaata
    45781 tttggaacac ctacttattt agcaaataca acttgggaag taaaaaaatg gtttgatgaa
    45841 gattcaaaaa aagttaattt agctggaaaa ttaggagctg tttttgctac atgtgattat
    45901 atatgtggag gtccagatgt tgcgatactt actatagtag gacatttgat ggttaaaggg
    45961 atgcttgtgt attctggagg tggttcttta ggaaagccat ttatacattt aggacatgta
    46021 cattcaattg aaggtccaga gttacaagat gagaaggcta taatatttgg tgaaagaatt
    46081 gctactaagg caaaagaact atttgcttaa tttctatatg catagtaatg attagagtaa
    46141 atttagggtt ttaatggaga tttgaagtaa aagttataaa ataaaaaatt ttccataaaa
    46201 ttactcaaga tgaattataa tataattata aataaattat attattaaaa tatttaaaat
    46261 caaacgtcaa tagcgaggga agtttatggt atggcatata gaatatgaga ttttttctgc
    46321 aattatagtt atttttttaa tggtatactt ttttagaagt aaatttatac ctactttaca
    46381 aaacaaaata tattgtgcac ttttaatatt ctcattttta tttataattt caaatatttt
    46441 aggttcattt tgtctaaata atatagacaa aattccaatt tttattactt ttttattgaa
    46501 tcagatatac ttattgttat tgccaatacc agcagcactt gtatcatttt atgttatggc
    46561 tattatttat caagatataa ggtatatgaa aaaaagatta ttatttttat ctataccact
    46621 tataatttgt ataatcttat ctattacaaa tccttttacg catattctat ttggattgtc
    46681 aaaagaaaca ggttatatac gaggttgtgg atatgttgca acttttgcat cttttttctt
    46741 ttatgcaata tattcagcat ttttatcttt taaatataga aaagcaatgc atgaatcaaa
    46801 aatatgggct attagaggtt ttcttatagt ttcaattgtg gcaattatct tacaggcaat
    46861 ttttttacag tatcttctta caggaactgc atgtgcatgt agtttacttt tgacatatct
    46921 ttctatgcag aatcgaggtc ttattattga tgaccttact ggtgtactca atagacaatc
    46981 ttttattcaa gagctagatt tgaatatcaa tactgaacaa tcaggattta ttattacaat
    47041 tgcgcttgat gattttaagt ttatgaatga aacttttgga acaaaaaatg gagatattgc
    47101 actaaaagaa gttggaaaat atttgattca gatacatgat gtaaaccatg tatatagaat
    47161 agggggagat atattttcta ttgttgtcaa tagtcaatta ggtatagaac cagatgatat
    47221 tatcagaaag atagaagaaa gatttaaaaa gccttggcta gtagaagaga taagttttaa
    47281 tttagcaact agtattgctg ttgtttatta cccagaaaat gcagaaacga cagaagatgt
    47341 aattgttgct atagatttct ctatacatga agcaaaacgc tctgagtcaa ggcgagttgt
    47401 ttatgcagat gcgtctataa gtgaaaagat aaagaagaaa catgttatta aaaattgttt
    47461 aaaagaagca ttaaaaaatg atgggtttga agtgtactac caacctatat tttctaatag
    47521 caggggcagg tttacttcag cagaggcatt acttaggcta gaacacaaag agttaggttt
    47581 tatacctcct gatgaattta ttccaattgc agaacaaaca ggattgatta actcaattgg
    47641 attagtagtc tttgagaagg tatgtagatt tatagctagt gatgagtttg aagatttaaa
    47701 attggataat atagctgtga atctttctgt agtacaatgt atgcaaaaaa acttagctga
    47761 tgatttactt tatcttatga ataaatataa tgtatcacca actaaattta aacttgaaat
    47821 tactgagact gtggctatgg gctcttttaa tataattaaa gaaacaatgg aaagattaat
    47881 tgatttaggt gttaagtttg cacttgatga ttttggaatt gcttattctg gagttactaa
    47941 tatgctttct ttaccatttt cagtaattaa gttagataag agtttgattt ggtctatgaa
    48001 tgaagatagt aggcataaac ttacagttga gacgattata gcacttataa ataaattgaa
    48061 tatgaaggct gtagcagaag gtgttgaaac cattaagtgt gctgaagatt taatagctat
    48121 ggagtgtgaa tatttgcaag ggtattattt ttctaaacca gttccagaag atgtttttaa
    48181 aaatttgttg aaagaaaata cttttacaag ttaacaccct ttattttttt aattacaact
    48241 ttgttcattg acactgccag cgatatatta taaaattaac acaataataa atattaaatt
    48301 tattatacat attattaata ttaattaatg gagtaaagat ttattatata ttttatttaa
    48361 ttatcctaaa gtgagaaagg aagatgtgac atgaataaaa agaaaatatt agtgccaatt
    48421 gatgggacag aaagaagtat gcattctttg gaatttataa aaggaatatt taaaaaagat
    48481 gaagttgaag tagaaataat gaatgtaaaa gaactagtat ttattgatgg catatccctg
    48541 gctgaagaga taaaaaattc tgaaaattta ggaagaagaa tcttggataa agcagcagag
    48601 ataatgggag agtatgatgt taaagtacat tttacttttg gatatccagg agatgagata
    48661 attagaaaag caaaagaaga taatgtagat tttatagtta tgacaaaatc tactaaaaaa
    48721 ggtcttacaa gaatgatagg ttcagtaaca gcaagtgtag ttaaacaagc aaaatgtata
    48781 gtgatgattg taccggagta gaattagaaa ggtatttgaa atatgaataa aaagatagta
    48841 gcagtttgtg aaagtgaaag aagtttaaat tctcttaaga cagctgcaaa tgatttagga
    48901 tttgatttag tttatgaaat tcaagaaaat gataatataa aaaatgaact ttctatttat
    48961 gatatagaag atgcatccat agtgttattt gttacaagtg attctataga aagcatagag
    49021 aagattgata gatttataga tagagaattt tatgaagttg atccaaagta tataatagag
    49081 gatgcaaaat ctatagtaag tgaaatttta atagacttaa attaatcgtg ggtgtcgcac
    49141 taagaataga gcgacgcctt tttatatatt ttacacaaga aataaagagg ttgtatcaaa
    49201 atatagataa gagtcttttt cattgttgaa atgatgaaaa tatatttgat ttcatacaaa
    49261 aaagagtacc tcataactaa aaagttcact ttgagacact ccatttttat atgttagttg
    49321 catttaaggt aaaatagaag tttaatcatg ttgaaataac tgaattacat cttctaaatg
    49381 attgtacatt gatgtataac ctaagtaaga gtctttacta tcaaatgcaa tcattatctt
    49441 gttatggtgg ctaatcatat ccttgagaga atttctatat ttaaaaccaa agtattggtt
    49501 agaagaaact gttttaggta ttaactcata atattttata aaaattggat tatgagaagc
    49561 taaaattatc tctttgtgaa attgcaagtc agcatcataa cattcttcta tcaaaccatg
    49621 agaaacctta tccaattcct ctattgactt taggtaagta tattttattt tttgaagttc
    49681 tttagatgta gcatttaaaa ttgctagttt agtagcttcg ctttcgataa gttgtcttgc
    49741 tcttaaagat tctacataaa attcttgtat gttatgattt ttatagattt caccatcatc
    49801 aaataggtca acttttttaa atccttcaac tataggactt gaataaggca cagctctaag
    49861 tattccttcc ttacagagta tatttatggc atttcttaca ggtattctac ttatgttaaa
    49921 taggtcagca atctttctct cacttggtaa tttactatta attttaaaag ttccatttag
    49981 aatgtttaat ttaatataat caataatttt ttcagtggca tttaagcttt tcataaaaac
    50041 tcctaaacat ataatttttt tatgattgta ttatatcata gttaagagtt aaatctactt
    50101 ttttataata tgtataagac aggatagagg ccaaaatagc tataatagcc aagataaagt
    50161 atccatattg tattgaagta tattccgata ttttaccaac tatcattgga aatacagaca
    50221 ttcctatagc ataaattgct tgaaaaaaac ccattgcagt agatttctga tgttttggta
    50281 catttttcat agactcgcta gtaaggtaag aaaggagtat acctgtagat atacctggta
    50341 gtacttgtag tacaaatatt atattaatat tttttattgt tggaactaaa aagcaatagg
    50401 ctgaaactaa aataaaaact aaaggaatcc aaaattttgg tccatactta gtacaaaatt
    50461 tagtagaagc aaagccagaa gaagcaacag cacacaccat gtaaaatata gaagatagac
    50521 ctatgagggt agaactagaa cctaagtttt taagaatttg agtagtaaat gacattgttg
    50581 tagacatttg aataccctgt tgaattaatg ccataaagga aaaaaataca agtcttttat
    50641 ccttgcagat agataaatat tttttaattg atattttttc aggtattttt ttagaattat
    50701 gattttttat atttaaggct agaatcgttc caataactga agacaataca ctcatgatac
    50761 ataaggctgt cattcctaat ttttcataaa ataaagtact cataacaaat ccaatcaaca
    50821 ttcctagatt gtttaccaat attatcgtac ttgtagcttt ttgttgatgt tctgtagaaa
    50881 aaaagttggt atagtgaacc atgtaagaaa tccaagttga tgatgatatt ccagataaaa
    50941 tgttagctat aagaaaacca ttgcctccag gagaaaagac tcttataaga gatgctaaac
    51001 cagcaaagaa aattccagac agtataaata ttttatgttt atttatggaa tcagcaaata
    51061 ctccaacagg aagccttaat aaacattgtg atattccata tgcacctatt attataccaa
    51121 tcatatttgt agatacatta catgcactta aatatggatt ttggaaaggt atatatacat
    51181 attgtgaaaa ccagaaaaat gtaacaatag aaagtaaaag taatttttga ttagatattt
    51241 ttttcatatt atcctccatg tggtattttt ataatgcaaa taatatacca tttagatttt
    51301 ttaatcaagt actttctcta ttaaaaaaaa tttaaatata taattattat ttacaaaaaa
    51361 attatacttt atagttgaat taagaaaacg cttatgatac tataaagtta acaaatttga
    51421 atatataaca gtagacgtgt tactggtaat gcaggcaaga tcgaatcaat ttttatagat
    51481 tatctataga gtactatggg tataatctat ttatttggtt cgattttttt gtttaattgg
    51541 ccataaaaca aaaagcctat agttgaaaat ggaggatttt ttatggatta taaaaatata
    51601 gctcaagaaa ttctactaaa tgtaggagga aaagagaatg taaatgaagt gactcattgt
    51661 atgacaagac ttagatttaa agtcaaaagc gcttctaaag tgaataagga taaattatct
    51721 aaaactgaag gagtaataac tgttgttgag agtatgggtc aaatacaagt tgtaattgga
    51781 aataaagtaa aaaaagtata tgatgaggtc ataaaaattg tacctcaatc aaataacata
    51841 ggtaaaaaga atgaaagtca agaaaagcaa ggtataatta attctatatt atcagcagta
    51901 gctggtatat ttacacctac aatacctgct atagcagggg ttggtatgat taaaggtatt
    51961 ttatcagttc ttgctatgta ttatatgaat aaaaatggag tagatataaa agaaacgcaa
    52021 agctatatta tcttaaatgc tatggctgat tctatattct attttatgcc tattatacta
    52081 ggatatactg cggctaaagt atttaatgca aataaaataa tatctatggt attaggagct
    52141 acactttgtt atccaacttt tactgctttg atggcaggtg aagaatctgt tagattttta
    52201 ggattggcag taacaaaagc tacgtataca tcatctgtta ttccaataat tattgctata
    52261 tgggctcttt catacgttga aaaagtatta gaaaagtata tacctgaggt aattaaaata
    52321 ataatggttc caactttatc attggtagta atgttacctg caacattatt tttatttgga
    52381 ccaatcggaa tttatatagg aaatgtcata aacttttctt ataagtatat ttatgaatta
    52441 agcccagcat tgtgtggagc atttgtaggt gggttatggt gtgtgttagt tatatttggt
    52501 gctcatagag cattattacc aataggtata agtgatgttg ctcaaacagg aagacaaaat
    52561 ttattggcgt ttgcaggagc tgcaaacttt tcacaagcag gggctgctct aggagtattc
    52621 tttaaaacta aaaatcaagg actaaaaaca atttctatgt ctgcaactat aacagcttta
    52681 tttggtataa ctgaacctgc aatatacggt gccaatctta gattaaaaaa acctatggta
    52741 tgtgccgtaa tatgtggtgc atttggaggt gcgataatgg gaatgggtgg agcttatggt
    52801 aatgcatttg caaaccaagg tgttttaact atacctgtat atgctgaagc aggtgcatta
    52861 ggtttcttaa gttatctagg tggttgtgct atagcatttt ttggttcagc tatttcaact
    52921 tatctagttg gatttgaaga tttggaagag tcttctaata aggaaaattc atctataaaa
    52981 gtagaaacga aggatggtgt tattgatata acttcacctg tagagggcga atgtatagaa
    53041 ttgtcagaag taaaggatga tgtatttgct tcaaaagcta tgggagaagg tatagctgtg
    53101 ttacctacaa aaggagtaat aacagcccct actgattgtg aagtagcttc tttatttcca
    53161 acattacatg caataggatt aaaattagat aatggagcag aaatgttaat acatgttgga
    53221 atcaatacag tagagttaaa tggtaaatac tttacaaaac atgttaatca aggagattta
    53281 gtgaaaaaag gtgataaact tatatccttt gatatagata aaataaaaaa ggctggttat
    53341 gatgtaacta cgccagttat agtaaacaat acatttgatt aaaaaatagc tacgcacact
//
LOCUS       DAGSRM010000017        53249 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.017, whole genome shotgun sequence.
ACCESSION   DAGSRM010000017 DAGSRM010000000
VERSION     DAGSRM010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 53249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53249
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.017"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            49..858
                     /locus_tag="MJE33_001122"
     CDS             49..858
                     /locus_tag="MJE33_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBY9647081.1"
                     /translation="MKDYYKIGEISKIYGIGRDSLMYYEEIGILRPVRDINGYRMYNI
                     SDIWKLNLIKEFRSLNFPMKKIKEYLDDRSIESTKNILNEELDLIDKKIAEFISHKEN
                     IIKRLSSIESVIQNTKIDEIEVVYIEKRKALELNADIKRDEDFDFLIQKLQKEYEDRF
                     NILGNNNIGSAFSTEAINKGIFNEFKSVFCFLESNEKVYNIVFDEGYYVTLNYTGSNS
                     NNKIYMEKVFKFIEENNYKIIADPIEIYKIDIHETGIVEEFVTEIQVPITK"
     gene            992..1231
                     /locus_tag="MJE33_001123"
     CDS             992..1231
                     /locus_tag="MJE33_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647082.1"
                     /translation="MKCSNCGSVNIEKGILTTGGIYAETIGLKYRGDGKLDKVINKTF
                     PVQSTIYSDLCLDCGEIVRTYIKDNTDRNWCKDKK"
     gene            complement(1419..1595)
                     /locus_tag="MJE33_001124"
     CDS             complement(1419..1595)
                     /locus_tag="MJE33_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647083.1"
                     /translation="MFRDEMDKCTHMLTAYISSLYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(1649..1745)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(2100..2774)
                     /locus_tag="MJE33_001125"
     CDS             complement(2100..2774)
                     /locus_tag="MJE33_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBY9647084.1"
                     /translation="MNHHILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     STDSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPL
                     IETLEI"
     gene            complement(2994..3548)
                     /locus_tag="MJE33_001126"
     CDS             complement(2994..3548)
                     /locus_tag="MJE33_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBY9647085.1"
                     /translation="MSIALILIDIQNDYFKNGKCELFQSEETAENAKKILLFFRKNNL
                     PVIHIQHISTDKTASFFLPNTHGSEINKIVFPLDNEEIITKHTPDSFFETDLQSILEK
                     KNITNLVICGMMSHMCIDTSVRTAKKLRYDITLISDACTTKNLSWNNIEINANTVHQV
                     FMASLQNSFADVKNTDKFLSNYHK"
     gene            complement(3626..3991)
                     /locus_tag="MJE33_001127"
     CDS             complement(3626..3991)
                     /locus_tag="MJE33_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY9647086.1"
                     /translation="MKIRAEYTCLLEIVHDIIKGKWKTILIFELRQSNKTFSELQHNI
                     YGINQKMLIQQLKELRDFGIIDKISSAGYPLHVEYFLTNRGKKMLKAVEIMQEIGIEY
                     MLEHGQQEILDNKGICYNK"
     gene            4456..5241
                     /locus_tag="MJE33_001128"
     CDS             4456..5241
                     /locus_tag="MJE33_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY9647087.1"
                     /translation="MIVKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     SQVEDGNITSIILKNKSTLIIYSFKNNFNIGEMKNHIKDLDVESISGKKCVIDRLISK
                     YKDFYEKLDNKFCVLKEIKEIDFSNMKEYKIENAQEKDIDEIGKLLNRSDYKVSKNYI
                     EERKVHLKEGNVRAYFIRNDDTMISTVSTGMETSFLAMVVSVSTDKRYRGKGLASYMV
                     YNLSKELLLEGKVPCLFYNNDVAGKIYHNIGYKEINEWTILFK"
     gene            complement(5338..6162)
                     /gene="pdaA"
                     /locus_tag="MJE33_001129"
     CDS             complement(5338..6162)
                     /gene="pdaA"
                     /locus_tag="MJE33_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBY9647088.1"
                     /translation="MKKDSLKKYIMIGAFALILFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYGAYYVGNPNEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPELIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEKEGYKLKTLDYLNNKK"
     gene            6383..9502
                     /locus_tag="MJE33_001130"
     CDS             6383..9502
                     /locus_tag="MJE33_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBY9647089.1"
                     /translation="MDNIISVLNDVVFLDIEVSGLDCLNSEILEVGAVKVKDWKIYTY
                     ESLIKNKFEVPVEVFSVCKNLDKNDLEIANEIELVEDRLVNFVEDSFIICHDLSLKKK
                     FLEYHMPKLKNKFIDLIELAVILEPYHKDYSLEYLKNTLTNCNSKVENRALSDAIDII
                     NIVNCLLVKFNNYEKTTLEPLSFKINSYLKKFNLPTWEWSKFLEEANYDLSNNINIKK
                     EYNIFDSKEEKKKERETLKILNEEEKNYEELLKYKTIWENKEGFTYEYRPGQYELTKT
                     IRELFRNSEDEEKIACIEAPTGIGKSVGYLLPAILEARINKKRLLISTDTKELQIQLI
                     NKDIPNVLDSLGLNGKVSYGYIKGKNNYICIDRLEAYIDDYESQNPTKGEILSLIFLK
                     RLVEGGKYGDIEEINYLVFDNFKEISTHLRNVSCDPNMCRPKKCKKDCLYKNRIEELK
                     EEHITVVNHSLLAKWPYKDEKPLENIIVDEAHNLTEKGYDFFSSIINSKSLRYLLQEI
                     YPYEFIQNSSFIYKKYSRNMRKIKAFDKFYNVLKIGREDKQKIARSINLIIEEIDSIL
                     KFGNCNEYNNVSNYNLRWELNLQIDEIVGKLKKDGIDTEISYRAYSEKIKLSCEKIIK
                     NLVSIIIIIYRNIDDDSIDKEADIYKFGKAKTRDLEDIKIIFEIFLEYDEKDDYARIV
                     EIDKNYNDFEFRVVPLKIADLFEENILSQLEKGIFLSATLSLSESMSYFKNTLGIDRV
                     KNVEKIIEPIFDYKNRVSVVGFSDICEYRNSEFPNEMSKIISNISKITEGHTLALFNS
                     KDRQEKTYEILKKYLHSFNLEIYADKKGIRHLNDLNRKCVVLGSKGCFEGVDIPGDGL
                     VCVTLDKLPNLNPKDPLYFTIMKKYGIDYYTINYPQMTIKVKQAMGRILRSKYDYGCF
                     VIFDVGTNISVLKRLEKDLHDCKISKVNSNEFYTYIRRHLNKYRSLILKSVIFDTIKA
                     LNVDAKMDNNDVDKDIIKKDINENIRQRAVKGEVYHIDIIKKDMKVKYFDRNYLINLD
                     IFMREEDKN"
     gene            9520..10119
                     /locus_tag="MJE33_001131"
     CDS             9520..10119
                     /locus_tag="MJE33_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HBY9647090.1"
                     /translation="MAKQKFYAVKKGKNIGVYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIDGSEKLSFQEDKEFIEAYVDGSYEHSVKMYGSGVVILKNNEVIKTYSEKGKEK
                     TLVSMRNVAGEIEASKIAMQYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKEGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
     gene            complement(10376..12505)
                     /locus_tag="MJE33_001132"
     CDS             complement(10376..12505)
                     /locus_tag="MJE33_001132"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBY9647091.1"
                     /translation="MPNLPSLGSKAPDFKANTTNGPIRLSDYKGNWIVLFSHPGDFTP
                     VCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIEDRD
                     MRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDALQT
                     SDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYNDEE
                     VSKKIDNTCSWKKEHTKNIENECNCEHEHHDYLNKALDCKQEHKTDIKDDCNHEKKHT
                     KNTNKVHNSKQDKFKDKSCDEMNFNYDKDESCDKINSSYNKEDSSYEDFYKHNYKNYD
                     YTSEKNTKKIAMKTLKDSKKLVRPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVD
                     AQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGFSDA
                     ALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLTAIR
                     DVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKHQAN
                     LFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDYINK
                     NGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYDQAN
                     ILARTMSIGINDPEVLKEELEGIYGQF"
     gene            12771..13019
                     /locus_tag="MJE33_001133"
     CDS             12771..13019
                     /locus_tag="MJE33_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647092.1"
                     /translation="MMICFAMTFALVLINSVMGVHIGLDYYFLMGSVLTVCLTIGIVL
                     CNARKIFDSKAIHKTRTKTVSNNKKQKSHSRDIKRKIS"
     gene            13185..14576
                     /locus_tag="MJE33_001134"
     CDS             13185..14576
                     /locus_tag="MJE33_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBY9647093.1"
                     /translation="MNFRDLFEKAVDFIDEKRKSIVAISLSTLGVIALIIVFFLSSNE
                     FSVGNEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELNKISIDVDDLLAQADRVREMYKEENTTYDIAINYIS
                     TVSILNGMGSNLDVYKQNIETIKESRDVYDSAVKDQKAYKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKDDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYDKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNTLDEKE
                     NKYKKIEILDIEKADRYVKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAISENGLNEY"
     gene            14691..16160
                     /locus_tag="MJE33_001135"
     CDS             14691..16160
                     /locus_tag="MJE33_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBY9647094.1"
                     /translation="MSNVNKKLVIAIGAVALVVIIFIGIMCMQFKGEKIAKNTYVNGV
                     NIGKLTKSQAKQELAKKYKLENVDFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SAFFGNLSKTISANFGKKSNLVVAINYDKNKLKAEMEKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDISKSMENFDNQTKKGNYKNELVVKATPAKVKKEQLANIDTNLGTY
                     STTFKTSQINRSINIKLATDNISNVLLMPGETFSFNKHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYAGLEIVNVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPN
                     TDENKNNQNTNNQNPNNQQKPETPPTDNKPNETQPQPQA"
     gene            16225..16818
                     /locus_tag="MJE33_001136"
     CDS             16225..16818
                     /locus_tag="MJE33_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HBY9647095.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            17000..18346
                     /locus_tag="MJE33_001137"
     CDS             17000..18346
                     /locus_tag="MJE33_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBY9647096.1"
                     /translation="MPRKILDISLIEDNKSDLTFFNIEEEDGDETLKALKQSYVTTNY
                     KEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKNYITSAINMVNKALEINP
                     KDIDILNLRGLLKLLKCDFAKSFESFYTALCYENNHISRKYVNLISSEDFKTFLGRYN
                     HSIRFINEELNYESIHILENIIEEEPELIEPYVILSLLYDKLGNVKKREGYLDKLKEL
                     DKDNPVFEKNEEEKEDTSKKEEIKKVKRKKSILPYIIVGCVLIGMGIYLIQSKKRIEN
                     LNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVKNKEPEREITVADEEDLYYQAL
                     NLKKNKEYEKAIDNFKSVVSSGKTKKYISESIYQLAITNKLLGNKDEAIKYYKKYINT
                     YTKNDQYYDDSYYELGMLYYDNGDLKNAQQTFYSLRSEVPDSMYNNSKIKEILSEK"
     gene            18528..18899
                     /locus_tag="MJE33_001138"
     CDS             18528..18899
                     /locus_tag="MJE33_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBY9647097.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
     gene            18892..19581
                     /locus_tag="MJE33_001139"
     CDS             18892..19581
                     /locus_tag="MJE33_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBY9647098.1"
                     /translation="MYNVLQTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIVGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIVGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGVESITVVAIIFTGIF
                     GGMVASTVFKLGNINHPVAKGIALGTSAHALGTTKAFELGEIEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
     gene            20101..20967
                     /locus_tag="MJE33_001140"
     CDS             20101..20967
                     /locus_tag="MJE33_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBY9647099.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISNIIPIHDILLQSVYGGILSGLGVGVVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSNEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            21209..22018
                     /locus_tag="MJE33_001141"
     CDS             21209..22018
                     /locus_tag="MJE33_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647100.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVNKEIRSIKNMKKRVLKRGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYVGKFLEGKMEGKGSYTFFVDEGT
                     VMEYIGEFKEDKKNGKGNFIFSNGNEYIGYFEEDMMSGIGNMLYSSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEVLYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            22162..23226
                     /locus_tag="MJE33_001142"
     CDS             22162..23226
                     /locus_tag="MJE33_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBY9647101.1"
                     /translation="MKRLIALVLVVLIVLFYSPELLKEGYDLKEKFFQSEIWNEFLDK
                     ININDNSKKDKKEKSQNLQSNNTEGENLGESDIKNFEKISLGDNLSYVLSSIGKPGRI
                     DISEYGFDWYVYNQYGKEFAMVGLENDEVVALYSNSINSCENQDIKLNQDRQTVRTKI
                     TPLKYKRKGNTRYIINSENQYDIISKEGKYITIFYDIHEENRVCSYLIIDKSTEDEFK
                     NLYPDDSEELKKCFELEVIDLVNSVRNQRGLNSLRYSEQATLSSRKHSEDMRDNNFFD
                     HVNKKNETPFDRMKREGIVYTSAGENIAAGQINAIYAHEAWMNSEGHRKNILGNYNNI
                     GVGVIFGGSYKTYYTQNFYK"
     gene            23254..23973
                     /locus_tag="MJE33_001143"
     CDS             23254..23973
                     /locus_tag="MJE33_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBY9647102.1"
                     /translation="MTSWKKKTVYKCLIAVALFCGIVLISNFSKVSALMMDTNGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKDLI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVQNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            23997..24581
                     /locus_tag="MJE33_001144"
     CDS             23997..24581
                     /locus_tag="MJE33_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HBY9647103.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGEAVVVKRNNEIKISDIEELNP
                     EVIVLSPGPCSPKEAGICIDIVEHFKGKKPILGICLGHQTIGHVFGGDIIKAQQPVHG
                     KVYSINHTNKGVFRGLKNPLNVTRYHSLIVDSNTVPKELEITAITDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLKNFITEARERVHM"
     gene            24583..25932
                     /gene="pabB"
                     /locus_tag="MJE33_001145"
     CDS             24583..25932
                     /gene="pabB"
                     /locus_tag="MJE33_001145"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HBY9647104.1"
                     /translation="MIREINTKLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSFISS
                     QPFKVLKYKDTDENPLEVLKEELHKYRVVNDTNLPFVGGAVGYLSYDLGNYIENLPRT
                     AVDDIEMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIELEEKIIDDIEQRILKEEKKG
                     IDSICYEEKEVTPIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETELTS
                     FELYRDLRRFSPAPFGAFLNFEDAHILSNSPERFIRCVNKRIETRPIKGTRPRGKDKE
                     EDLRLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNHLVS
                     TVVGELKDDKDATDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYIGFN
                     GDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYYEE"
     gene            25922..26665
                     /locus_tag="MJE33_001146"
     CDS             25922..26665
                     /locus_tag="MJE33_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HBY9647105.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIRVKNGIAVDLDIHIERMLSSIN
                     CLDLDINYKKDFLINEIVTYIKKENVINKALRITVFDEGYNISIRDIPYNQETYEKGF
                     KLAISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEGVILECSMSNIF
                     FIKGERVYTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIVKLLE"
     gene            26699..27400
                     /locus_tag="MJE33_001147"
     CDS             26699..27400
                     /locus_tag="MJE33_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647106.1"
                     /translation="MIGCIYEVYNEEMTKIIENQNTLAVYLVGSSKDVDFTLYDVVIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDVNYFSKDGVKKLLSNREYFFVKEMKDAKVLF
                     DRENISHIIKDISRDIYLEGPSKMSLEEKSFIKQDIGAKILNLKNKEKFDVFEYHFLT
                     NLYLKDIIIGYFNINDKWVPKDKKLLKVLKKENNELFELVSKVSENYDYQRLLDVYNY
                     IFKEIETKEVIKLIF"
     gene            27426..27998
                     /gene="folE"
                     /locus_tag="MJE33_001148"
     CDS             27426..27998
                     /gene="folE"
                     /locus_tag="MJE33_001148"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HBY9647107.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLSEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            28062..28853
                     /gene="folP"
                     /locus_tag="MJE33_001149"
     CDS             28062..28853
                     /gene="folP"
                     /locus_tag="MJE33_001149"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBY9647108.1"
                     /translation="MFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDMVDQG
                     ADIIDLGGESTRPGHSYVDSDEELRRVIPVIKKLKQELDIPISIDTYKADVAEEALKL
                     GVTMVNDVWGLRKDKNMASVIGKYDAEVCIMHNQDGTNYDKDIMESIKDFFKVSIEMA
                     MSCGVKKEKIVLDPGVGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGKVLPV
                     EPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            28864..29229
                     /gene="folB"
                     /locus_tag="MJE33_001150"
     CDS             28864..29229
                     /gene="folB"
                     /locus_tag="MJE33_001150"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HBY9647109.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYNVVKDITENKQFNLLEALAENIAEEVLNKFILINGVMVRVRKPEA
                     PVNGIYDYFGVEIRRTRDE"
     gene            29222..29728
                     /gene="folK"
                     /locus_tag="MJE33_001151"
     CDS             29222..29728
                     /gene="folK"
                     /locus_tag="MJE33_001151"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HBY9647110.1"
                     /translation="MNKAYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERELHRERIVHWGPRTIDVDVLFFN
                     DVVSTDERLTIPHPRIQDRAFVLIPLMDLNEELIINEKTIKEHLNLLSAEEREEVKEL
                     VGYERKPI"
     gene            29709..30494
                     /gene="aroF"
                     /locus_tag="MJE33_001152"
     CDS             29709..30494
                     /gene="aroF"
                     /locus_tag="MJE33_001152"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBY9647111.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKSQGANILRGGAYKPRTSPNSFQGLKKEGLEILKAVKDEVGMAVITELMDVRD
                     MDELYSISDIIQIGSRNMQNFTLLSEVGKQNKPVMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
     gene            30749..32539
                     /locus_tag="MJE33_001153"
     CDS             30749..32539
                     /locus_tag="MJE33_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBY9647112.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLECGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            32557..33723
                     /gene="rpoD"
                     /locus_tag="MJE33_001154"
     CDS             32557..33723
                     /gene="rpoD"
                     /locus_tag="MJE33_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBY9647113.1"
                     /translation="MENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETELDK
                     DQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHDDSSAIEI
                     ETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEANL
                     RLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIMKI
                     AQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQKV
                     LKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            33888..34577
                     /locus_tag="MJE33_001155"
     CDS             33888..34577
                     /locus_tag="MJE33_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBY9647114.1"
                     /translation="MKLTDRLLKIASLVSDGKKIADIGTDHGYIPVYLLKEGKVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFNDFIEKKIKTYSSIVNKLEGKNGEAIDKKRKESE
                     VAIKKLENLIK"
     gene            34591..35688
                     /locus_tag="MJE33_001156"
     CDS             34591..35688
                     /locus_tag="MJE33_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBY9647115.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSGDLKGRLIQKLIKNDISLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDITKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSIEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKLDKSMTLESLSRKIKEKLNMKHIRVVGNLS
                     TDITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEFSEIEVIKSNVYLNPFSTI"
     gene            35751..36323
                     /locus_tag="MJE33_001157"
     CDS             35751..36323
                     /locus_tag="MJE33_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBY9647116.1"
                     /translation="MKVAVIFHSVCGSTYLLAREYKEALEEMNIEVDIFRVSDEVAKT
                     LPQYYLINSKEYKDEFESINVIKSGKEILDYDAIFMGSPTYYGNVSGPMKMFMDSFSD
                     IWVGTPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSVRGGYPAYG
                     ILHIAGDNSDIRPNDDAKIGIRDYLKRLNI"
     gene            complement(36383..36850)
                     /locus_tag="MJE33_001158"
     CDS             complement(36383..36850)
                     /locus_tag="MJE33_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBY9647117.1"
                     /translation="MFKEMRLKKREMTKEDTVEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFNDSIYFHCARNGHKLDNISKNNKVSFLVVANESVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDMNLTTVVKIEIDHISGKASRL"
     gene            complement(37236..37712)
                     /locus_tag="MJE33_001159"
     CDS             complement(37236..37712)
                     /locus_tag="MJE33_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBY9647118.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLSVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            complement(37832..38923)
                     /locus_tag="MJE33_001160"
     CDS             complement(37832..38923)
                     /locus_tag="MJE33_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HBY9647119.1"
                     /translation="MSKIECLFTIFPVLILMYATFYFMPYFVGKKHIYGVSIDQEHKN
                     YNYFIKLDKEFKKLLSLGFIIDFILVFILVFTFNKLELSYFIGIIGFLLYESILYIHT
                     HKKAKKLKSEIYSKLGHIDIDSKLIVDMDFINKKNKIIKKFKIIYLIPILFTLGMSIF
                     IAFNYNQLPDSIATHWNINGSPDVFIDKSFLNVFKLIGTDFCLMVLLYITSIGSIKSR
                     IKIDTNRIEESRVENIKYLNKIGYLFLILMIIMTTQFFTTLLSIKTKLSTAMNITTLL
                     VIIYLIATYINSPNLKFNSSYSPEEDEKYWIAGIMYNNPNDPSLMVNKRFGIGWTINF
                     GNPLGKILYIAIALLLIFSLFSLIKSLLL"
     gene            complement(38916..39305)
                     /locus_tag="MJE33_001161"
     CDS             complement(38916..39305)
                     /locus_tag="MJE33_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY9647120.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLDLELLVAKS
                     YLSGISKEEFLSLSSSLFDKYEVKYYE"
     gene            39497..39942
                     /locus_tag="MJE33_001162"
                     /pseudo
     CDS             39497..39942
                     /locus_tag="MJE33_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            40206..40931
                     /locus_tag="MJE33_001163"
     CDS             40206..40931
                     /locus_tag="MJE33_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY9647121.1"
                     /translation="MKRKEIVMLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSG
                     KEMISSNEYSMILLDVTLPDGTGYELCQYIRGFSQVPIIFLTACDEEVNIVMGLDIGG
                     DDYITKPFRIRELISRIKAVLRRKGNTSEENKKILKFGDLSIYTLEARVYKNDKEIFL
                     TSVEYKLLLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCE
                     PIYIETVRGIGYKWIGSENSVSI"
     gene            40918..42162
                     /locus_tag="MJE33_001164"
     CDS             40918..42162
                     /locus_tag="MJE33_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY9647122.1"
                     /translation="MFLYNPEVKKFLSKYVTLMFIVIIISIGFSIINVSLTKEMVVRN
                     NQAIIGTLSSKYPNLESQIVDIITQGKSMENIDYGQKILIKYNYDKSIRINSEPIISK
                     LVLETIKINIILVCIIFILIFVLVVRYFKSIYNDLSDMTKYVYYSSEGKPFDMKNKNQ
                     EGQIGLLKTELLKMTTILNEKVELLKREKIFLNNTISDISHQLKTPMTSLIMLNDLLY
                     NDVPYEVKIDFLNKIKNQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHRAML
                     SMKIPMEIKNQKLTIEGNDNVSYIGDIDWSVEALVNIIKNCVEHTPEFGNIIITYKEN
                     PLFSELIIKDEGEGIDKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQNGDIY
                     VNSEKGQGTEFHIIFHKMYDSD"
     gene            42242..42919
                     /locus_tag="MJE33_001165"
     CDS             42242..42919
                     /locus_tag="MJE33_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9647123.1"
                     /translation="MEILRVENLTKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDREYFDDLMKTLGLIDREKHLPNQLSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            42920..45481
                     /locus_tag="MJE33_001166"
     CDS             42920..45481
                     /locus_tag="MJE33_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9647124.1"
                     /translation="MNLYTLLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQMRETIKNDGSFHVTFYDVNRKNVEYVTKSAEIEKHAFTKQLGYSKLENSENAILS
                     IKQYDKNAINGYKVSIKEGRFPAKEGEIVLSESIINLIDDKLKIGDKITLKVGDMFDS
                     SKKKIDSMTFHEGDYIANEKDRTFKVVGIIEKPGFERYNGIATAIAYFNPMNNYNDTI
                     NVSVAVRNPKDVYKISNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYSNINNSI
                     KSIIIAVTILVIICTIATVYNSFSISINERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGLISGTVAIDLLFKIINKYFTESVVTKMSLQIVYNPIITIVSIIIVLLTIF
                     ISILLPAISASNISHLNVIKNTGEYKVGKIKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     IITLFSLMVSIIIFISFSGFTLLLLRGEEIRNSQRKYDLYLTAKNTARSVEDTISELE
                     DIDGIKNFELATGQYVSIRVSENKINKSKEDLIRKYYQKYKIGDSYEYDFSNNELIFP
                     GDFAVKNKINNLVQGSFNKERAIEENGVILVRKSAFEENGKKGVVELTNYKVGDTVNC
                     EYLDENALSKKVKVKILAITDEERLGLGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNNFNFYDESASNESEKQDLKVIKIFVYGFIVVISLVSVTNI
                     LNTVSTSINLRKRELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGIILIM
                     NKLISGVIEFSPIIPWTAIVICIVSVFIITFIAGYIPMSKLNKENIIDNIRRESI"
     gene            45702..46976
                     /locus_tag="MJE33_001167"
     CDS             45702..46976
                     /locus_tag="MJE33_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBY9647125.1"
                     /translation="MITISHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQ
                     EAKLVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPP
                     MAGFFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLT
                     NDNEVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSL
                     INNKFARRVDKIECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFG
                     NLHPVQTTSFIPNINHENCVKCGKCITACPIDAISKVKEDGKEYIKIDEDRCLGCGVC
                     VRNCHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNNALASHRAMGAIL
                     SAILKLEPAKKILASKQLKSVYLDKLLSMNDK"
     gene            complement(47137..50178)
                     /locus_tag="MJE33_001168"
     CDS             complement(47137..50178)
                     /locus_tag="MJE33_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
                     /protein_id="HBY9647126.1"
                     /translation="MFKQRLSKLLSSTLVLSMLFTAAPNITFADNTKDNSEKYQSSDI
                     ELHDYSKNAESYTKTKALAKEKIQTLLSKYGAVSAQYALIDNGKIEISGNGGVYSKQD
                     NKNLNKDNMYSIASISKMFTTTAVMKLVDDGKLNLDTPVVKYIPEFKMADDRYKEITP
                     RMLLNHSSGLMGSSFKNTILLADNDSYGHDNFLKELQKQRLKAKPGAFSVYCNDGFTL
                     AEILVERVSGMSFTNFLDKYINNPLNLQNTKTPENSFDSSKLAKAYVPYWEDAVPQDN
                     LNAIGAGGLYSSAENLCTFAQTFMKNSNGILSPASVKAMENKEYLNGLWPEGEDSILG
                     YGLGWDCVNTYPFNQYNLKALTKGGDSLLFHSNLIVLPDENMAVAVLSSGGSSQLNEI
                     IGQEILLSALKEKGKIKEIKPDKTFSKPQQVKMPSSLKENSGLYASSNMIKVDVNDNG
                     TLTVSSPYIENGPEDKYVYIGQDRFVSEKGNSCLKFVKEKNNITYLNMSSYDDVPGLG
                     QTASLYYVAQKVDDNNISNSVKEVWKKRSGKGYYLVDEKYTSQSYMFGSVKASFSLSD
                     ETPGYIVNTKIMDENNSNAFIEIPGVIGRDLSDIKLHKENGTEYLSFGTLTYVSEDSI
                     TNLPAEKSFTCELESNGYAKWYKIGDDIANKKIEVNLPQNSAFAVYDDKGVPVNYSLV
                     TKNNRVRLPKGGVIVFLGSPNARFEVTYQDEVNASALTGTDRYETSIKISQAGWENAE
                     NAVLINDSAIADALAATPFAYKKNAPILLTGSSQINEKTLAELKRLKVKNVYVVGGET
                     SINEKSLDTIKSNNISVSRISGSDRYQTSMNIAKELNNISNISKISVVNGEKGLADAV
                     SIGAVSAQNDMPIILTNENSNITEINNLFKNKKIDKSYVIGGEYTVSKNIESKLQNPQ
                     RISGSTRNETNAKVIKEFYKDSKIDNLYVAKNGMNKQDDLIDGLSVGVLAGKTKSPVM
                     LVGNSLDYNQKELFKTMRFKSVTQIGGNGNENSFKQIKEIA"
     gene            50385..50684
                     /locus_tag="MJE33_001169"
     CDS             50385..50684
                     /locus_tag="MJE33_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBY9647127.1"
                     /translation="MYKNINKKQLKEMMKNEKDVLLLDVRTKDEFKEYKIENAINISL
                     QELINNIDEIYDYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYNLNSGIIDYMS"
     gene            complement(50922..53150)
                     /locus_tag="MJE33_001170"
     CDS             complement(50922..53150)
                     /locus_tag="MJE33_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBY9647128.1"
                     /translation="MRHLKKTNSLKEILNWNNIKNLRPSKGLKNILEIFKSDLKDIRG
                     NYAVLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGD
                     QLIEQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITDDVKKGEI
                     IYTVNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLT
                     KVENSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSSDL
                     KKFLEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLS
                     EKLSNLQSLNDTLVDFLTKLNQLTSNNRLDDVIDNLEDSSNKIDSSISTLNDIKNKVI
                     SGQQPSISALNNVLSLSNGIGRINLNILNNFDSKISKPINSIFANSIKVANDIITVLD
                     KAEAKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIDTLSKISNGEDMK
                     KLVSLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALL
                     STSVHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIVLLMFTS
                     MVFNFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYA
                     IGATREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRVIKPFNDKFGDSNLT
                     GH"
BASE COUNT        21170 a         5742 c         8735 g        17602 t
ORIGIN      
        1 atgttgattt aaaataaaaa taagatttcg gaggagttta ttatataaat gaaagattac
       61 tataaaatag gagagatatc taaaatatat ggaataggta gagattcatt aatgtattat
      121 gaagaaatag gcattctaag accagttaga gatataaatg gatatagaat gtataatata
      181 tcagatatat ggaaattaaa tttaataaaa gaatttagaa gcttgaactt tccaatgaag
      241 aaaataaaag aatacctaga tgacagaagt atagagtcta cgaaaaatat tttaaatgaa
      301 gaactcgatt taattgataa aaaaatagct gaatttataa gccataagga aaatattata
      361 aaaagattga gttcaattga aagtgtaatt caaaatacaa aaatagatga gatagaggtt
      421 gtatatatag aaaaaagaaa agctttagaa ttaaatgctg atataaagag ggatgaagac
      481 ttcgattttt taattcaaaa acttcagaaa gaatatgaag atagatttaa tatacttgga
      541 aacaacaata ttggttctgc cttttctact gaagcaatta ataaaggtat atttaatgag
      601 tttaaatctg tattttgttt tctggaaagt aatgagaaag tatataatat agtttttgat
      661 gaaggatatt atgtaacttt aaattatact gggagtaatt ctaataataa gatatatatg
      721 gagaaggtgt ttaaatttat agaagaaaat aattataaga taattgctga ccctatagaa
      781 atctataaga tagatataca tgagactgga attgtagagg agtttgtaac ggaaattcaa
      841 gttcctataa ctaaatagct atatttcaat ggattagatg gtaaatagaa tgaaaaaatg
      901 ttgatttgcc actgtgttat aaaatcggta gcaaattata ttttaaatta taagatagca
      961 tgaaaaaata attgtttagg gggttataaa tgtgaaatgt tctaattgtg gaagtgtaaa
     1021 tattgaaaag ggaattttaa caactggtgg catatatgct gaaactattg gattaaaata
     1081 tagaggagat ggaaaattag ataaagttat aaataaaact tttccagtac aatctactat
     1141 atattcagat ttatgtttag attgtggaga aatagttaga acctatataa aggataatac
     1201 tgataggaat tggtgtaaag ataagaaata gaataatttt tgaagtatat taatatttga
     1261 agttgaattt tattaaagat acaagtgaaa aagtataaat gggaagaaat tgttatatgg
     1321 aagaaaactt tgcatatagg ggagattagt tttttaatat aataacattt ttaaatgtag
     1381 ataaaactaa aaagggttct ctagaaaaca atttctattt aattatactt acttaccaat
     1441 accatcactt gatgtaaaca agattctact acattttctc cgtactctag tataaaatca
     1501 tctagctgtg tatctataaa atcacaataa tcatataaac tactgatata agcagttaac
     1561 atgtgtgtac atttatccat ttcatctcta aacatatata ttactatctc cctaaaaaac
     1621 tttcataaaa caaaaagaac aatatctttt tggcaactgg ctgacataac tcatagttta
     1681 ttttataagc gttagtccct atagctttgc gtcactaaat ttctctagtt ttgccgattt
     1741 agttttattc tacaactaaa ataatacact aggataagtt attgttcaac atacttgtca
     1801 gaaaattgaa atatttaaca gtatcttaac aatatgaaat ctatttttca ctcaagaaat
     1861 aaactataca tgtagaatat ttaataaaaa ttatatttag ttttgtattg aagtttttgt
     1921 attaaaaagt ttagttcaca agagttactt ggctagataa tagatgaaca aaaatatctt
     1981 gaaaaaatat atatgaatgg caaaaaacaa gggatgaatt ttatatagac tcatcccgta
     2041 attatttata tattgacctt gattaaatat attattattt ttaagcttca atttttatat
     2101 tatatttcta aagtttctat aagaggatta tcgctaaata attttaataa atttgttttt
     2161 ttatatcctt taggtaactt agtatatata actacttcta accaatcccc atcaaggcgt
     2221 ttaatattca tgtttataat ttcaatatta gattttttaa atttaccttc aatatattct
     2281 atggaatcag tgctgttaga aattttaaga gaaacttgct ctgctgtagg gagtttcatc
     2341 catttcctat ctctatgaag tgttatctgt acaataacaa taatgatact tgaaacaata
     2401 ccaactaagt ataatccaga gccaatggca agacctgtac cagctgttgc ccatatacca
     2461 gcagctgtag taagaccact tactgattga tttctaataa atattaatcc agcacctaaa
     2521 aaacctatac cactgacaac ttgtgctgca acgcgggctg ggtctagacc aatacctgga
     2581 gtacccacta tatcattaaa tccatacttg gaaacaatga ttataagtgc tgaagctaat
     2641 gccactataa aatgagtgcg tattccagct tcttttagtc tattctttct ctcatatcca
     2701 attaaagccc cacagaaacc agcagtaatg attcttgcta tatattcaaa ttgaatcaat
     2761 atatgatggt tcatttatct agcaccccct tctatataat attatatata ctataatact
     2821 acatttttta tgtgaatata gtaaagttta aaatttatat aatagttata tatgaatata
     2881 aaatgtataa acgtatatta tgtatataaa aatgatagaa agcatctaat tttaaatgat
     2941 taaagagcaa gcttaaataa atcaaacttg ctcttttaac atagtgtatg tttttatttg
     3001 tgatagttag ataaaaattt atctgtgttt tttacatctg caaaactgtt ctgtagtgat
     3061 gccatgaata cttgatgtac agtatttgca ttaatttcta tattattcca agataaattc
     3121 ttcgttgtac atgcatcgct tattagagtt atgtcgtatc gaagtttctt tgctgttctt
     3181 acacttgtat caatacacat atggctcatc attccacaga ttactagatt agtaatattt
     3241 tttttctcaa ggatactttg caaatctgtt tcaaagaaac tatctggtgt gtgtttagtt
     3301 ataatttctt cattatctag tggaaaaaca attttattta tttctgaacc atgagtatta
     3361 ggaagaaaaa aagaagctgt tttatctgtt gatatatgct gtatatgaat aactggtaaa
     3421 ttgttttttc taaaaaacag tagtattttt ttagcatttt cagcagtttc ttctgattga
     3481 aataattcac attttccatt tttaaaataa tcattttgaa tatcaatcaa tattaaagct
     3541 atagacatga ttatacctcc tatttatttt tattataata ctattgtaaa aaatgtaaat
     3601 tacttacttt ttggtatgta taggattact tattgtagca aattccttta ttatctagaa
     3661 tttcttgttg accatgttca agcatatatt cgattcctat ttcttgcatt atttccactg
     3721 cttttaacat ttttttacca cgattagtaa gaaaatattc aacatgtaat ggatatccag
     3781 cagaacttat tttatcaata atcccaaaat cacgtaactc ttttaattgt tgaatcaaca
     3841 ttttttgatt tattccataa atattatgtt gcaattcaga aaatgtttta ttagattgtc
     3901 ttaattcaaa tataaggata gttttccatt ttcctttgat tatgtcatgt acaatttcaa
     3961 gaagacatgt atattcagct cttattttca tagcatatca cccccagtaa aatgataata
     4021 aattatatca cttgtcttgt gatatgacaa atatatatga actgtatata aattttatgt
     4081 tttattaagt tctaaatttc ttctattgta gttcatattt agtacataaa taattagtaa
     4141 gatataagta aataaatata taacatgact atagaaattt aactttaaaa taacttatga
     4201 taatttagag gaggaatata tatgaatata tcaagaaaag ctatgaaaat aatagaatta
     4261 gcacaaaaaa ttgcaaataa gagaggtatt tcagtagaag aagcttggag tgaagcagta
     4321 acagaatata aaaataaata cgaacatatt gcatagatat ttaaaaattt agaatatttg
     4381 gtagaatata atgattttaa atgatactat aaattataag gtatatcatt attaacatag
     4441 aaggaggata tctgcttgat tgtaaaaata gataatactt tagaaaaaga attttggcaa
     4501 tatgtatcac atgaagaaag tttaaattta ttcataattg gatacgttga gaattatgga
     4561 tttagctctc aatatcaaga tatatggtct caggtagaag atggaaatat aacctctata
     4621 atattaaaaa ataagtcaac acttataatt tatagtttta aaaataattt taatatagga
     4681 gaaatgaaga atcatataaa agatttagat gtagaaagca taagtggaaa aaaatgtgta
     4741 atagatagac taataagtaa atacaaagat ttttatgaaa aactagataa taaattttgt
     4801 gtattaaaag aaataaaaga aatagatttt tccaacatga aggagtataa aatagaaaat
     4861 gctcaggaaa aagatattga tgaaatagga aaacttttaa ataggtcaga ctataaagta
     4921 agtaaaaatt acatagaaga aagaaaagtt catttaaaag agggaaatgt aagagcatat
     4981 tttattagaa atgatgatac tatgattagc actgtgtcaa ctggtatgga aactagtttt
     5041 ttggcaatgg tggtctctgt aagtacagat aaaagatata ggggaaaagg acttgcaagt
     5101 tatatggttt acaatttgag taaagaattg ttgttagaag gaaaagttcc atgtcttttt
     5161 tataataatg atgtagcagg taaaatatat cataatatag gatacaaaga gataaatgaa
     5221 tggacaattc tttttaaata gctataaaaa ataaaaaact ctatcgagaa ttaaagtatt
     5281 tataaataac tatattttta ctataattca tgatagagtt tttttaaatt tatgatacta
     5341 ttttttatta tttaaataat caagtgtttt aagtttataa ccttcttttt cccaatgtgt
     5401 aagaacctca tctaatatct cagtatttgt tgatgaattt ggatgcagta aaactattgc
     5461 accaggatga gttcttgaat aaattttgtt tttggcaaat tcatgagtag gttgtttctt
     5521 ttcataccaa tctacataag caaaactcca aaatattgat ttatatccta aatcttgtgt
     5581 atattttaaa gattgttcgc taaatttacc cattggaggt ctaaaatact taggcatttc
     5641 ttttccagta acatctttgt atgctttttc tacacttgta atttcttctt tgaatttttc
     5701 aaaatctgta atacctgcca ttgatggatg gctttttgaa tgattacaaa ctaaatgtcc
     5761 ttctttttcc atacgcttta ttaactctgg attgcttttt atataacttt caactacgaa
     5821 aaattgagct ttagcattgt gttttttaag cgtatctaat aattttggag tgtttccact
     5881 ttcatatcca gcatcaaatg atagatatat aactttttca ttgggatttc ctacataata
     5941 agctccgtat tttttaaaaa aatcagcttc ttttataggt gatggctgtt taccatcctc
     6001 cctagggtta aaataccaat catattcatg tgtgtcgagt gttggactag aaatttgagt
     6061 ttttgtttta tctaatgatt taaaatttat actagcaatt ccaaaaagta ttagagcaaa
     6121 tgctcctatc ataatgtatt tttttaggct atcttttttc acaatttcac ctcttattta
     6181 aaatttaaag tatatttctt ataatatagt ttgaatattt atagttaaaa atattcacat
     6241 atacaaataa taaatgaaag aagtttactg taagatataa agttgatatt aattatctta
     6301 aataaagtgt atataaatga taaaatataa agagaaatga ttgtaaataa gttaggctgg
     6361 tttaacttga agggatataa aaatggataa tataataagt gtattgaatg atgttgtttt
     6421 tttagatata gaagttagtg gattagattg tttaaattca gaaattttag aagttggagc
     6481 tgtcaaggtt aaagactgga aaatatatac ctatgaatca ttaataaaaa acaaatttga
     6541 ggtacctgta gaagtattct cagtatgtaa aaatttagat aaaaatgatt tagaaatagc
     6601 taatgaaata gaactggtgg aagatagact tgtaaatttt gtagaagata gtttcattat
     6661 atgtcatgat ttaagtctga aaaaaaagtt tcttgagtat catatgccaa agttaaaaaa
     6721 caaatttatt gatttaattg aattagcagt tattttagaa ccatatcata aagattatag
     6781 cttagaatac ttaaaaaata ctttaacaaa ttgtaattct aaagtggaaa atagagcttt
     6841 atcagatgct attgatataa taaatattgt aaattgcctt ttagtaaaat ttaataacta
     6901 tgaaaaaact actttggagc cattgagttt taaaataaat tcatatctta aaaaatttaa
     6961 tcttccaaca tgggaatgga gcaaattttt agaagaggca aattatgatt tatcaaataa
     7021 tataaatata aaaaaagaat ataatatatt tgactcaaaa gaagaaaaga agaaggaaag
     7081 agaaacctta aaaattttaa atgaagaaga aaaaaattat gaagaattat taaaatacaa
     7141 aactatttgg gagaataaag aaggatttac atatgaatat agacctggtc aatatgagct
     7201 tactaaaaca ataagagagt tgtttagaaa tagcgaagat gaagaaaaaa ttgcatgtat
     7261 agaggcacca actggtatag gtaaaagtgt aggatattta ttgcctgcaa tattagaggc
     7321 tagaattaat aaaaaaagat tattaatttc tactgacaca aaggaattgc agatacaatt
     7381 aataaataaa gatattccaa atgtgttaga ttctttgggt ctaaatggaa aagtaagtta
     7441 cggatatata aagggaaaaa ataactatat ttgtatagat agactggaag catatataga
     7501 tgattatgag tctcaaaacc ctactaaagg agaaatttta tctttaatat ttttgaagag
     7561 attagttgaa ggtggcaagt atggagatat agaagaaata aattatttag tgtttgacaa
     7621 cttcaaggaa ataagtactc acttaagaaa tgtaagttgt gacccaaata tgtgcagacc
     7681 taaaaaatgc aaaaaagatt gtttatataa aaacagaata gaagaattaa aagaagaaca
     7741 tataacagta gtaaatcatt cactattagc taaatggcca tataaggatg aaaaaccttt
     7801 agaaaatata atagtagatg aagcacataa tttgactgaa aaaggatatg atttcttttc
     7861 aagcattata aattcaaagt ctttaaggta tttattacaa gaaatttatc cttacgagtt
     7921 tattcaaaat agtagcttta tatataaaaa atatagtaga aatatgagaa aaataaaggc
     7981 ttttgataaa ttttataatg tattaaaaat tggaagagaa gataaacaaa aaattgctag
     8041 aagtataaat ctcattattg aagagataga ttcaatatta aagtttggta attgtaatga
     8101 atataataat gtaagtaatt ataatttaag atgggaactg aatttgcaaa tagacgaaat
     8161 tgtgggaaaa ctaaaaaagg atggtataga tactgaaata agttatagag cctatagtga
     8221 aaagataaag ttgagttgtg agaaaataat aaaaaattta gtttcaataa taataataat
     8281 ttataggaat attgatgatg atagtataga taaagaagca gatatatata aatttggaaa
     8341 ggcaaagaca agagatttag aggatataaa gattatattt gaaatctttt tagagtatga
     8401 tgaaaaggat gattatgcaa ggatagttga aattgataag aattataatg actttgaatt
     8461 tcgagtagtg ccattaaaaa tagcagattt atttgaagaa aatattttat ctcaattaga
     8521 aaaaggaata tttttatcag caactttgag tttatctgaa agtatgtctt attttaaaaa
     8581 tacattagga atagacagag ttaagaatgt ggaaaagatt atagaaccaa tttttgatta
     8641 taaaaataga gtctctgtag ttggattttc tgatatttgt gagtacagaa attctgaatt
     8701 tccaaatgag atgagtaaaa ttatatctaa tatatcaaag attacagaag gtcatacatt
     8761 agctcttttt aatagtaaag atagacaaga aaaaacttat gaaatattga aaaaatactt
     8821 acatagtttt aacttagaaa tttatgctga taaaaagggg attagacacc ttaacgattt
     8881 aaatagaaaa tgtgtagtac ttggctcaaa aggatgtttt gaaggcgtag acattccagg
     8941 ggatggtttg gtttgtgtaa ctttagacaa gcttccgaat ctaaatccaa aagacccttt
     9001 gtattttact ataatgaaga agtatggtat agactactat actataaatt atcctcaaat
     9061 gaccataaag gtaaagcaag ctatggggag aattttaaga agcaaatatg actatggatg
     9121 ttttgtgatt tttgatgtag gaactaatat aagtgtgtta aagagattag agaaagattt
     9181 acacgattgt aaaatttcaa aggttaatag caatgagttt tatacttata taaggcgaca
     9241 tttaaataaa tatagaagtt taatactaaa atctgttata tttgatacta taaaagcgtt
     9301 gaatgttgat gctaaaatgg ataacaatga tgttgataag gatattataa aaaaagatat
     9361 aaatgaaaat ataagacaaa gagctgtaaa aggagaagtg taccatattg atattataaa
     9421 aaaagatatg aaagtaaaat attttgatag aaactatctt ataaatttag atatatttat
     9481 gagagaagaa gataaaaatt aagagtggga gtgatatatt tggcaaaaca aaagttttat
     9541 gctgtgaaaa aaggtaaaaa tataggtgta tataatacat gggatgagtg caaaaaacaa
     9601 gtaaatggat tctcaggagc tgaatataag agtttttcta cttttcaaga agctaaagaa
     9661 tatattgatg ggtcagaaaa attgagtttt caagaagata aggagtttat agaagcatat
     9721 gtagatggaa gttatgaaca ttcagtaaaa atgtatggtt cgggtgttgt gattttaaaa
     9781 aataatgaag ttataaaaac ttatagtgaa aaaggaaaag agaaaacttt agtaagtatg
     9841 agaaatgtag ctggagagat agaggcttct aaaattgcaa tgcagtactg tattgacaat
     9901 aatgttcaaa acttgatttt atactttgac tatgaaggga ttgaaaagtg gtgtactgga
     9961 gtttggaaaa caaataaaga aggaactata gcatataaaa atttctatga cagtattaag
    10021 aataagctta atgtaaaatt tacaaaggtt aaagctcatt ctggaaataa gtataatgag
    10081 gaagcagata aattagctaa aaaagctatt ggtgtataat aaaaaaatat ttatacataa
    10141 ttacatggta taagagtata tctaaattcc tactttataa ttaaacaact tgtaattaaa
    10201 caacttgtaa ttaaacaact tataattaaa taaattttga ttaaaaaagc agattgtatt
    10261 atgaaaaaat aagtatctaa tttataaaca attaataata gtattaatta gatacaactt
    10321 atttaatata ataaaatctg ctttttatat aactataaaa ttttcctaca ttctattaga
    10381 attgcccata aataccttca agttcttctt ttaatacttc agggtcattt ataccaatag
    10441 acatggttct agccagtata tttgcttggt cataagttga tgtccatgag aatacaccac
    10501 ctagacaatt tctaagaaca tattgagttt ttaagaatat tgataaagca tcatcatatg
    10561 acattgcaaa atctccatcc ttaactaaat atggaacttg agcagtatta tcaaatctat
    10621 attcatatcc atttttattt atataatccc ttctaagctc atcataagtt cttgttatag
    10681 tagcacctaa tcttccataa aatggtatac caagaaggat tttttcagaa ggcattccag
    10741 cattctcaag atttcttacc attgcatcaa cactatatcc tggcaaagat aagtctgaat
    10801 caaaaagatt tgcttgatgt tttctaccat ttgggcctgt ttcacctgct gtaaaatcat
    10861 aactcataag attaaaataa tctattatag gagctatttt atctatttca gcacttgaat
    10921 ttatatatcc tctatctcct gttccagcta cactaagcca tttatcatct cctataacat
    10981 ctcttatggc agttagtaag agtgtaaagt tttctctatc ttgaggtctt gatgttattc
    11041 cagatgcact acttccagga tattcccagt ctatatctat tccatctaaa gcatattcat
    11101 ttatcatctg attgacctgt cttgcaaaat tatatctaga tgtaggtgtt aatgctgcat
    11161 cagagaatcc ttcagctccc caacctccaa tagctacaat tacttttaag ctaggttttt
    11221 cacctttaag agcaagtaat tgatttaaaa atctaggata aggaataaac aaatttccag
    11281 actggtctat ctcagcaaaa gcaaatataa ctgcatctaa tagttgagca tctacattag
    11341 ttggatttcc aagaacatat tctgctacaa ttggatttat atctggacag tttgcatttt
    11401 caactattgg attgtatggg tctgttattt gtggtctaac taatttttta gaatctttta
    11461 aagttttcat agctattttt ttagtatttt tttcgctagt ataatcgtag tttttataat
    11521 tatgtttata gaaatcttca taactactat cttctttgtt atagctagaa tttattttgt
    11581 cacaagattc atctttatca tagttaaaat tcatttcatc acaagactta tctttaaact
    11641 tatcttgttt ggagttgtga actttgttag tatttttagt atgttttttc tcatgattgc
    11701 aatcatcttt aatatcagtc ttgtgttctt gtttacagtc aagagctttg ttcaggtagt
    11761 catgatgttc atgttcacag ttacattcat tttcaatgtt tttagtatgt tcttttttcc
    11821 agctacaagt attatcaatt ttcttgctca cttcttcatc attataatta tctggtacaa
    11881 aacataagta ccagtccata catgaatatt tcttattaca actgttaact ctatttttta
    11941 attccttata gtttttagga taaggtaaaa tcactggcat tccaggaaac cagtttgcag
    12001 gagtaactat attatctcta tcactagtct gaagtgcatc tactattcta agtatttctg
    12061 gaatatttct tcctgtagtt agtggataat aaagaatcgt tcttagaatt tgattattat
    12121 ctataataaa tacagagcga acagttgatg tatcactcat tggttttgat atcatgccgt
    12181 ataacttggc aattctcata tctctatctt ctataatagg aaatggaatt tctacacctg
    12241 taagtaaaga aatattatac atccaagcta aatgtgaact gttactatca acacttagac
    12301 caattagttc tgtatttctt tttttaaatt cgtcataata tttagcaaaa cataaaaatt
    12361 ctgtagtaca aactggtgta aaatcaccag gatgtgaaaa taaaacaatc caattaccct
    12421 tatagtcaga gagtctaata ggaccatttg ttgtattggc tttaaaatca ggagcctttg
    12481 accctaaact tggcaaattt ggcatgtaaa tcacccctca ataaattatc atttatttta
    12541 ttctattaat taatacatat tagtgttact attttattaa aagttaagta atccttatgt
    12601 caaaaaaaga tatttattaa aaagatttaa tattttccta atataaatga caatagtaat
    12661 tgctattttt ttactaattt ggtatagtaa tggataaggg gtagtgtcaa ttttagtgat
    12721 tatacgaggt gagtggattg aaaaaaattc taagaatttt agttatctcc atgatgatat
    12781 gttttgcaat gacttttgca ttagtgttaa taaattcagt tatgggagta catataggat
    12841 tggattatta ctttttgatg ggaagtgtgc ttacagtttg tttaacaatt ggaattgttt
    12901 tatgcaatgc tagaaaaata tttgatagca aagctataca caagacaaga acgaaaactg
    12961 tcagtaataa taaaaaacaa aagagtcatt ctagggatat aaaaagaaag atatcataat
    13021 ttatgtaaag gaaatattta tgtataaaat aatgattgat aggtaaaata aaaacacgat
    13081 ttaacttgtg tttttatttt ttttcaagaa ttatgatata ataaccaata agaaatttag
    13141 tttaccataa aatataaaga ttattaaatg gagagggaac tataatgaat tttagagatt
    13201 tatttgagaa ggctgtagat tttattgatg aaaaaaggaa atctatagtt gcgatttcat
    13261 tatcaacatt aggtgtaatt gctttgataa tagtattttt cttaagtagt aacgagttta
    13321 gtgtaggaaa tgaagctaat gagttgttaa agattataga gaaaagacag tattctatag
    13381 ctgttgatta ctacacaagt atagaaaaaa agttttctga ttcaaaaatg gaaaggttta
    13441 ataagtctgt ttctaaaaaa ataaataaat tgctattaaa tagtggtgat aaatatttag
    13501 atggagacat aagtcaagaa agttttattg gattaataaa taccgtgaaa gaattaaaca
    13561 aaataagtat agatgtggat gacttattag ctcaagctga tagagttaga gaaatgtata
    13621 aagaagaaaa tactacttat gatattgcca ttaactacat aagtactgtt tcaatattaa
    13681 atggtatggg tagtaaccta gatgtataca aacagaatat agaaacaatc aaagagtcta
    13741 gagatgttta tgactcagca gtaaaagacc aaaaagcata taagtattat gaagctattg
    13801 aaaagtataa taaagtatta aaagaagatg aaaagtatta cagtatggct caaaatggga
    13861 aagaacaatg tattgaagaa atgtatgatt actatataag tagagctgaa gaggcaaata
    13921 cttctggaga ttatgaaaga gcattacaat atattgagta tttaaaagac gattattcag
    13981 atgatgaaaa ggtacagagt ttagaaaaaa aatataaaaa gaatttatca ttgtatactt
    14041 taacatctga tgacattata aatgtaatag ctaaaaaaag tggcaaagac aaggcaaatc
    14101 tatctataaa ttcacttcca caaatgataa aaaataataa atactattat gctgaagtat
    14161 atgagtatga taaattaata aatgaagttt tggtaagtgc aaaaactaaa gatttatatt
    14221 catataaaga tggtaaaaaa gattacaaag tagattatgg tgatggatac tttagaatat
    14281 tagaagatgg aagttatcaa tttggtatta caaaagataa agctaagttt gtgttaacta
    14341 atactcttga tgaaaaagaa aataagtata aaaagattga gatattagat atagagaaag
    14401 cagatagata tgtaaaaagt aaaaagagtt tagaagaatt gtttggaaaa tataaaaata
    14461 tttattatta tgctgtagta aataaaggct tatttagagg caaagaagtt tatgctatta
    14521 atatttacaa tgaaaaaata tatgccatat cagaaaatgg actaaatgaa tattaattaa
    14581 taatattaaa ttaatatata attaaaaagt ttttggggtt ttaaggcgca ttatgtagta
    14641 atataataat agggatatat aaataatttt attgtaagga gaagtatggg atgagtaatg
    14701 tgaacaaaaa gctagttata gctattggag ctgtagcatt agtagtaata atttttatag
    14761 gcataatgtg catgcaattt aaaggggaga aaatagcaaa aaacacatat gttaatggag
    14821 taaatattgg aaaattaact aaatcacaag caaaacagga attagctaaa aaatacaaat
    14881 tagagaatgt tgattttaat tacaatgata aaagctggaa agtaaagagc aaagatttaa
    14941 atttatctac tgacctggat aaaacagtag aaaacgcata taatttaaat agaaagagtg
    15001 ctttctttgg caatctatca aaaactattt cagcaaattt tggcaaaaaa agcaacttag
    15061 tagttgcgat aaattatgat aaaaataaat taaaagcaga aatggaaaag atagctaaag
    15121 aaatagatgt agatgtaaaa gatgctacac ttgatataag tggtgaaaaa gtcaaggtta
    15181 taccagattc tgatggtctt aaaatggata tttctaagag tatggagaat tttgataatc
    15241 aaactaaaaa aggtaactat aaaaatgagt tagtagtaaa agctactcca gcaaaagtaa
    15301 aaaaagagca acttgctaat atagatacaa atttaggtac gtattcaaca acatttaaaa
    15361 cttctcaaat aaatagaagt ataaacataa aattagcaac agataatata agtaatgtat
    15421 tacttatgcc aggagaaact ttctcattta ataaacatac agggaaaaga agtaaggaaa
    15481 atggatataa aagtgcacct gtaataatgg aaggtgagat ggaagaagat tatggtggag
    15541 gagtttgtca agtttcatct acgttataca attctgtgct atacgcaggt ttagaaattg
    15601 ttaatgtaaa aaatcatact ataccatcaa gttatgttcc aaaaggaaga gatgcaactg
    15661 ttgcagatag tggaatagat ttcttattta aaaataatct aaagcatcca gtttacataa
    15721 aaaattacgt ttcaggaaat caaattgttt gcaatatata tggaagtgca gaagataaac
    15781 aaaatataac aatatcaaca aaattagatg gcgtttcaca aactacaatg aaaagagtta
    15841 atgaccctac aatgcctaaa ggtaaagaaa aagtagacaa aagtggaaga aatgcatatt
    15901 ctgtatcaac atatagaaca tttaatgatg caaatggtaa aaaaattaaa actgaaaaaa
    15961 tagctaattc atattatcct aagaaggaag gtattatatt agttgggact atggaaccaa
    16021 aaccagagga aaaaccaaat acagacgaaa ataagaataa tcaaaataca aacaatcaaa
    16081 acccaaataa tcaacaaaaa ccagaaacac caccaactga taataagcct aatgagacac
    16141 aaccacaacc gcaagcttaa agttctcaag aataggattt atgtagagta ataaaaaata
    16201 taaatctaaa taggagggat tactttgaat ttaaataata taacgaatta cgttcaaagt
    16261 atttgcgaga atatttctaa tgtcttggat gtagatgtta ctttagttac aaaagattta
    16321 actaggatag caggtacagg aatattcaga ggtaaaatag gagagacaat atcagagaag
    16381 tcagtgtact ctcttgtttt aaaagatggg aaatcctatg ttataaataa agaaatagaa
    16441 gagaactgta ataaatgtgc aaatagagaa aattgtaaag agttagctga tatatgtact
    16501 cccgttaaaa tgggagagca tatacttgga atcttgggta tagctgcgtt taatgaagaa
    16561 caaaaagaaa agatactttc aaaagataag gagttaactg cttttgtaag gagtatgtct
    16621 gatttaatat cttttaaact agatgaactt tataaacaag aagttaagac tatagatgac
    16681 cttgagaggg aagctataga aaatgccata aagaaatatg gaagtaatac agaaggtatg
    16741 aaaaaagtag ctgaggcatt aaatataggt atagctacac tctatagaaa agtaaaaaaa
    16801 ttcaacatta aaagttaata gtattgaaaa agaggtaacc tttttatgaa aagaagagga
    16861 ttaccttttt tattatgaga atggtggaaa aatatgtata gttttgactt gacatattaa
    16921 atatgttatc ttaaatatat attttagagt ggaggtgagt attattttag ttaaagatat
    16981 aaaacaagac atagaaagca tgcctagaaa aattttagat atatctttga tagaagataa
    17041 taaaagtgat ttgacttttt ttaatataga agaagaagac ggtgatgaaa cattaaaagc
    17101 attaaaacag agttatgtga ctacaaatta taaagaagaa actctattaa acttgcaagg
    17161 agctttaaat tcaaagtcag atacaacgtc taaggagtta aaattagaat catataaaag
    17221 atataacaat gcattggatt tagcatataa aaattatata actagtgcca ttaatatggt
    17281 taataaagct ctggagataa atcctaaaga cattgatata ctaaatctta gaggtctttt
    17341 aaaattatta aaatgtgatt ttgctaaatc atttgagagt ttttatactg ctttatgtta
    17401 tgaaaataat catatatcta gaaaatatgt aaatctaatt tcatctgaag attttaaaac
    17461 ttttttagga agatacaatc attctataag atttataaat gaagaattaa attatgagtc
    17521 aatacatatt ttagagaaca taattgaaga agaacctgaa ctaatagagc catatgtaat
    17581 tttatctttg ttatatgata aactaggtaa tgtaaaaaag agagaaggat atttagacaa
    17641 acttaaagag ctagataaag ataatcctgt atttgaaaaa aatgaagaag aaaaagaaga
    17701 tacaagtaaa aaggaagaaa taaaaaaagt aaaaagaaag aaaagtattt tgccatatat
    17761 tatagttggc tgtgttttaa taggtatggg gatatatttg attcaaagca aaaagagaat
    17821 agaaaacttg aataaccaaa taagtagtaa agaggaaaag ttaagtgaaa ctgataaaaa
    17881 gttaggagaa acgagtaaaa aattagaaaa aacgaataag gaattaaatg aggttaaaaa
    17941 taaagaacca gaaagagaga taactgtagc tgatgaggaa gatttatatt atcaagctct
    18001 aaatttaaag aaaaacaaag aatatgaaaa agccatagat aattttaaaa gtgtagtttc
    18061 ttctggtaaa actaaaaaat atatttctga gtcaatatat caattagcaa taacaaataa
    18121 attattagga aataaggatg aagctataaa atattacaaa aaatatataa atacttatac
    18181 taaaaatgac cagtattatg atgattcata ttacgagttg ggtatgcttt attatgataa
    18241 tggcgattta aaaaatgcac aacagacttt ttatagtttg agaagtgaag ttccagacag
    18301 tatgtataac aattcaaaaa taaaagaaat tttaagtgaa aaataggttt atatagttaa
    18361 gttttttgaa acaaccaagt atgtatttta ttaaattaaa ataaaaataa atttaaaaag
    18421 agattgtgtc aaattataac tttctatttt aaagaaagat atttgcttgt tgataaaact
    18481 acaaatatat gatttgaagc aatctcttta atttttggag gtggaaaatg aaagttttca
    18541 atcaattggt aataatttta tctatctggg taataggaga gtatataagt tcctttatac
    18601 aaggaattat acttattcct gggagtatag ttggtatgct attattattt ctactactac
    18661 aatttaaagt tttagacttg agtagtatag aaaatgtaag tggatttttc ctcgataata
    18721 tggctatatt ttttattcca gcaggtgtat cacttataaa gtcattagac ctaataaggg
    18781 aaaatgtatt agtgctttta ttagtcatat gtttaagtac actaatagta atgtatacta
    18841 ctggaattat tgtggagaaa atgataaaaa agaaggaaga aggacaaaaa aatgtataat
    18901 gtattacaga caccagtatt tggtataata gtaacaatag tttttttcaa tttaggaaga
    18961 tacattcaac gaaaaacaag taatcctata tgtaaccctt tattaattgc aatcgtagga
    19021 ataatattgt ttttaagcat atctaaaatc ccttatgaga attacaagat aggagctgat
    19081 agtctaaatt tctttttagg tcctgtcaca gtagtacttg cagttccact ttataaacaa
    19141 tttgaattat ttaaaaagca tatgtttgag attattgtag gaataggttg tggtatagtg
    19201 atttcttttg tatctgtatt gataattggc aaaatagcta attcagatgt aagtataata
    19261 aattcactaa ttccaaaatc tataacaaca ccaatgggaa tatctttagc aaattcttta
    19321 aatggtgtag aatcaataac agttgtagcc ataattttta caggtatttt tggtggtatg
    19381 gttgcttcta cagtattcaa actggggaat ataaatcatc cagttgctaa aggaattgct
    19441 cttggaacat ctgcacatgc tttaggaact acaaaggctt ttgaacttgg agaaatagaa
    19501 ggagctatga gtggtgtttc tattggagtc tctggtacaa ttacagtaat actaattcca
    19561 attataatga attttatata aaaaataaaa ttaagatgag taccttgaat acttaaaaca
    19621 aaataaaata aaaaaaattt aaaataaaaa ctgaacgaaa aaaatgcaaa taatgaaata
    19681 aaaaaatcat gtactgaaat tataaaaaga aaaatttcat ttaggaatta attataaaga
    19741 gacaattact aaacttgaat ttgctaaaat aaaataaatt actgctaaaa tagatattta
    19801 gaaaaaaata ctataaattt actatattgc aagttaaata ataagcagta tcatattatg
    19861 ctagaaaaag tttcatttaa ggcataaatg agatgatttg aacgttttta aagattagaa
    19921 tataaaagat tgcactagac atatatgaaa aagataatat aatagatgat gtaatagatg
    19981 acagttcaag aaattataaa aaaaatttat ttgaaatatt atctattaca tcaggaataa
    20041 tatatgtaag agtaagcttt taaaattcta aagatttaga aagattgaag ggagaaagaa
    20101 atgagtgaag ttaagaatac cctacaaaaa catccagttt taagatattc aacaatgcta
    20161 tttgggatgt tgatgacatc tataggaata aatgggttct taagaccagc acatctgctt
    20221 agtggaggtg ctacaggtat agcaacatct ataaactatt tgacaaatat aaatgtaggt
    20281 ttattaacat ttttaatcaa tattccaata tttatcttag ggttcatata ccttgaaaaa
    20341 gagttttgta tttcaagttt ggtaaatatg attgtatttt cattattatt aggagcaact
    20401 caggaaataa gtaatattat acctatacat gatatattgc ttcaaagtgt atatggtggt
    20461 atattatctg gtttaggagt cggagttgta tttagaacta ggtcttctca aggtggtaca
    20521 gatataatag ctgcaatact aaaaattaag aagaatatag aaatgaaaga cactgcttta
    20581 gcaataaatg gcttgatagt acttacagga agttttttat ttgggttgga tttagcatta
    20641 tacactctta taggtctatt tctaaatgcg tattcaatga gttttattaa agatgcaatg
    20701 aattaccaaa aatctgttat ggtaatgtca aatgaagtcg atttaatagc agaagatata
    20761 atgaagagtt tagttagagg tgtaactttt ttagatgcag aaggtgcata cactcatcaa
    20821 aaaaagaaga ttatatatac aatagtttca tcaaatgaga taccaaagat aaaagatata
    20881 gctttgaagt atgataaaaa ggcctttata tctgtaaatg atgttacaga agtaaaggga
    20941 agagggttta aagcaaaaga tttatagaaa attttacaag ctgggagttt ataatgtaaa
    21001 tttgtatatt atatctctca gcttatttaa atttagtata cgaaaataaa ggttttatta
    21061 atgatttgtg tatcacttta aaagatatca ttttaaaatt aaaattgaaa aaattactta
    21121 atatgtagaa aacatgacca ctattaggat ataataaatt taatagattt agtaaaatac
    21181 ctaggaataa cgttggagga atggcattat gaatttatct aaagaatatg ttaatttgta
    21241 tgataattat ttgaaatata taaatgaagt aaacaaagag attagaagta taaagaacat
    21301 gaagaaacgt gttttaaaaa ggggcgattt tgctccggaa aatgaagtat tagaattgga
    21361 aggaaaaaca atacgcgata tagatgatgt cgatacaaaa aaaccaaaaa ataaaatcta
    21421 taaattttca aatggggatg tatatgtagg gaaattctta gagggaaaga tggaggggaa
    21481 aggctcatat actttttttg tagatgaagg aacagttatg gagtacatag gagaatttaa
    21541 agaagataaa aaaaatggaa aaggaaactt tatattttca aatggaaatg aatatatagg
    21601 ttattttgag gaagatatga tgagcggtat tggaaatatg ttatatagta gtaaagatga
    21661 atatattgga acttggaaaa acggaaaaaa agatggaaaa ggtatttata agtggagtga
    21721 tggatgtata tatgcaggtg aatttaaagg tggaaagatg gaagggtacg gtatttgcta
    21781 taatagtaaa ggggaagtac tttatgaagg agaatggaag aataatttga ttcatggtaa
    21841 gggaacttat atatgggaaa aaggtaaaaa gtatataggt gaatttatgc atggtaaaaa
    21901 acatgggcaa ggaacttttt atctaaataa tgaattggtt tatgaaggga cttggaaatt
    21961 tgataagcca tctatatttg atagaacatt agatgaaata ttttccttga gactgtagct
    22021 ttatctaatt agtagataaa gcttttttct aatacttact gtaaataggt atgaatgata
    22081 ttgattagta tacactatca atattaactt aataaatgca atattaaaaa attttgattg
    22141 attttaagaa ggagagatat attgaagaga ttaattgctt tagtgttagt agtacttatt
    22201 gttttatttt atagtcctga attattaaaa gaaggatatg atttaaaaga aaagtttttt
    22261 caaagtgaaa tatggaatga atttttagac aaaataaata taaatgataa ttctaagaaa
    22321 gataaaaaag aaaaaagtca aaatttacaa tcaaataata ctgaagggga gaacttagga
    22381 gagtcagata taaagaattt tgaaaaaata agcttaggag ataatttaag ctatgtttta
    22441 tcaagtatcg gcaaacctgg tagaattgat attagtgaat atggttttga ttggtatgta
    22501 tataatcaat atggaaaaga atttgcaatg gttggacttg aaaatgatga agtagtagca
    22561 ctttatagta attctattaa ttcatgtgaa aatcaagata taaaactaaa tcaagataga
    22621 caaacagtga gaactaagat tacaccatta aaatataaga gaaaaggaaa taccagatat
    22681 ataataaact cagaaaatca atatgatata ataagtaagg aaggtaagta tattacaatt
    22741 ttctacgata ttcatgaaga aaatagagtt tgttcatatt taataattga taaaagcact
    22801 gaagatgagt ttaaaaattt atatccagat gatagcgaag aacttaaaaa atgttttgaa
    22861 cttgaagtta tagaccttgt aaatagtgtt agaaatcaaa gagggttaaa ctcattgaga
    22921 tacagtgaac aggcaacttt gagttcaaga aaacatagtg aagatatgag agataataat
    22981 ttttttgacc atgtaaataa gaagaatgaa actccatttg acagaatgaa aagagaaggt
    23041 atagtatata cttcagctgg agaaaacata gcagcaggac agattaatgc aatatatgcg
    23101 catgaagctt ggatgaactc tgaaggacat agaaaaaata ttttggggaa ctataataat
    23161 attggagttg gagtaatatt tggaggaagc tataaaacat attacactca aaatttttat
    23221 aaataactaa aatatttgaa aggaagttaa aaaatgacat catggaaaaa aaagacagta
    23281 tataagtgct taatagcagt agccttgttt tgtggaatag tgttaatatc taattttagt
    23341 aaagtttctg ctttgatgat ggatactaat ggaaatgttt tgattaaaca tggttcaagg
    23401 gaaaaaaagc taatagcaat tacctttgat gatggtcctc atcctaaaga aactagtcaa
    23461 gttttagatg tattaaagaa atacaatgta aaagctactt tttttatagc tggaaaacat
    23521 gcaaaatggt ataaagaacc attagtaaga gcaagtaaag aaggtcacga gataggaaat
    23581 catactttca atcatcctga tataagtaat ttaagttcta gtcagataga agaggagatt
    23641 gttaagtgtg aagacatttt gaaggaagta actgggaaaa aacctacatt atttagacca
    23701 ccattcggaa gttatagaga aaaagactta atagaaattg caaaaaagca tgactataaa
    23761 gttgttttat ggacaggtgt agatgtgaaa gattggaaaa atccaggagc taatagcatt
    23821 gcagataaaa taattaataa agtacaaaat ggagatataa tacttttgca tgattatgca
    23881 actaatgata cagttgaagc tttagatatg tttattccaa aaatgattga aaaaggtttt
    23941 aaatttgtaa cagtttctga gttgataaaa taatttttag gggggagatt tacagtatga
    24001 ttcttatgat agacaattat gattcttttg tatacaacct agtccaatat atagaagaat
    24061 taggggaagc agttgtagta aaaagaaata atgaaatcaa aataagtgac attgaagaac
    24121 taaatccaga ggtaattgtg ttatctccag gaccgtgttc tccaaaagaa gctggtattt
    24181 gcatagatat tgttgagcat tttaagggga aaaaacctat actaggaatt tgtttaggtc
    24241 accaaactat tggacatgtt tttggaggag atattataaa agcacaacag cctgtgcatg
    24301 gaaaagtata tagtatcaac catactaata aaggagtttt taggggactt aaaaatcctt
    24361 taaatgtcac taggtatcat tcactaatag ttgactccaa tacagttcca aaagaattag
    24421 aaattacagc aattactgat aaaggggaaa taatgggaat taggcataag aaatatttaa
    24481 tagaaggagt acagtttcat ccagaagcta ttttgtcaga gtatggtcat gaaatgctta
    24541 aaaattttat aacagaagct agagaaaggg tacatatgta atatgataag agagataaat
    24601 actaaattaa attcttttga gatatttact atttttagaa atgaacatga cagttttata
    24661 ttagatagtg ctatggataa agaaaaattg ggaagatatt cttttataag tagccaacca
    24721 tttaaggtat taaagtataa agatactgat gaaaatcctt tagaagtttt aaaagaagaa
    24781 ctacataagt atagggttgt aaatgatact aatcttccat ttgtaggagg tgctgtggga
    24841 tatttatctt atgatttagg taattacata gaaaatttac ctagaactgc tgtagatgat
    24901 attgagatgc ctgatatgta ttttggattt tataatcatg taatagttat agaccatctt
    24961 gtgcaaaaaa cttatatagc tactccaaac atagatatcg aattagaaga aaagataata
    25021 gatgatatag aacaaagaat attaaaagaa gagaaaaaag gtatagatag catatgttat
    25081 gaagaaaaag aagtaacacc tataagactt aaatctaatt ttacaaagga agaatttaaa
    25141 aatgcagttc aaagtgttag agagtacata agacaaggag atatatatca agctaattta
    25201 acacaaaggt tcagtggaga aacagaactt acaagctttg aattgtatag ggacttaaga
    25261 agatttagcc cagcaccatt tggggcattc ttaaactttg aagatgcaca tatattatca
    25321 aattctccag aaagatttat aagatgtgtt aataagagaa tagaaactag accaataaaa
    25381 ggtactcgtc caagagggaa agataaagaa gaagatttaa gacttcaaca agaacttaga
    25441 aatagtgaaa aagatagagc tgaactactt atgatagtag atttagaaag aaatgatatt
    25501 gggagaatat ctaaaactgg aagtgttaaa gttccagagc tatttgtaat tgaaccatat
    25561 gcaaatgtaa atcacttagt ttccacagtt gtaggtgagt taaaagatga taaagatgct
    25621 actgatgtaa taaaagcaac ttttccaggg gggtctataa caggagcacc taagattaga
    25681 gcaatggaaa ttatagacga attagagcca acgcaaagaa atgtatatac tggttcaata
    25741 ggttatatag gttttaatgg tgatatggat tttaatatag caattagaac aataatcaaa
    25801 aatgacaaaa aagtctactt tcaagttgga ggaggaatga cttgggattc tgacccagat
    25861 gaagaatatc aagaaacact tgataaagct aaatccataa tgaaggcttt gaggggatat
    25921 tatgaagaat aatgtaggtt ttgatagtag tttaagtaaa tttggaattg gtttgtttga
    25981 aactattaga gttaaaaatg gaatagcagt agatttagat atccatatag aaagaatgct
    26041 aagctcaata aattgtttgg atttagatat aaactataaa aaagatttct tgataaatga
    26101 aattgttaca tatataaaaa aagaaaatgt cattaataaa gcacttagaa ttactgtttt
    26161 tgatgaaggt tataatatat ctattaggga tattccatat aatcaagaga cgtatgagaa
    26221 ggggtttaaa ttagctatat ctccaatagt tagggggaat agtttaattc atagacataa
    26281 gactactaat tattttgaaa acatatatac caaaaatatc gcaaataaga ctggttataa
    26341 tgatggtata tttttaaatt cagaaggtgt aatattagaa tgttctatga gcaatatttt
    26401 ctttataaaa ggagaaagag tgtacactcc tagtgataga ttaccgatat taaatggaat
    26461 aataaaaaaa aggattatag agatttgtga cgaattacat atagagttga tagaaaatga
    26521 aattaatata tcagaaatta gtagttttga ttttgttttt gtcacaaata gtcttatggg
    26581 cgctataaag gttacagaaa ttgacaaaat aaaatttaat aaagaaaatg ttgtttttga
    26641 taaaatagtg aagttattag aataaattaa aatattaaca atttgttgga gacaaaagat
    26701 gataggttgt atttatgaag tatacaatga agagatgact aaaataatag aaaaccaaaa
    26761 cactttggct gtatatttag tgggttctag taaagatgta gattttactc tttatgatgt
    26821 agtgataaac gatattgatg tgttcgtttt tgtaaaagaa ggggaaaaac aagagagaat
    26881 actgtttaaa aaaaagggaa tagaatttga tgtaaattac ttttcaaaag atggtgtaaa
    26941 aaaattatta tcaaataggg aatatttctt tgtgaaagaa atgaaagatg ctaaagtact
    27001 gtttgatagg gagaatatat cacatattat aaaagatata agtagagata tttacttaga
    27061 aggacctagt aaaatgtcat tagaggaaaa gtcttttata aagcaagata ttggagcaaa
    27121 aatattgaat ttaaaaaata aagaaaaatt tgatgttttt gaatatcatt ttttgacgaa
    27181 tttatattta aaagatataa taattgggta ctttaatata aatgataaat gggtaccaaa
    27241 agacaaaaaa ttgctaaaag ttttaaaaaa agaaaataat gagctttttg aactagtgtc
    27301 aaaagtaagt gaaaattatg actatcaaag acttttagat gtttataatt atatttttaa
    27361 agaaattgag acaaaagagg ttataaaact aatcttctag tcacaaatca atggggggag
    27421 tttaaatgaa taaagttgat aaagaaaaga tacaacatgc agtaagagaa atactagagg
    27481 caattgggga agacccagat agagaaggtc taatcgaaac tccaaataga gttgctagga
    27541 tgtatgaaga gatattctct ggtcttagtg aagaaccaag agaccattta aaagttcttt
    27601 ttgcagatga aaaacatgaa gagttagtat tggtaaaaga tattccattt tattcatgtt
    27661 gtgagcatca tctagttcct ttctttggaa aagctcatat tgcttattta ccaaaaggag
    27721 gaagacttac agggctttct aaacttgcta gagtaataga tacattggca aaaagaccac
    27781 aattacaaga aagaatcact aaaaatgctg cagatataat aatggaagaa ttacaacctt
    27841 atggtgtctt agtagtagta gaagctgaac atatgtgcat gacaatgaga ggcgtaaaaa
    27901 aaccaggttc aaaaactgtt acatcagcag tgagaggaat atttgaaaag gacattgcgt
    27961 ctagagccga ggctatgagt ttgattacta tgaagtagta atttattata gatataatca
    28021 tttattgaga taaaatttaa tttaagggag tgtcattaat tgtgtttgat tatggtaaaa
    28081 gaacttatat aatgggaata ttaaatgtaa ctccagatag tttttctgat ggaggagatt
    28141 ttaataactt ggacatagca atacaacatg caaaagatat ggttgaccaa ggtgcagata
    28201 taatagacct aggaggagag tctacacgcc caggtcatag ctatgtagac tcagacgaag
    28261 agctaagaag agtaatccct gtaataaaaa aacttaaaca ggaattggat ataccaatat
    28321 caatagacac ttataaggca gatgttgcag aagaagcatt aaaattaggt gtcactatgg
    28381 taaatgatgt ttggggtctt agaaaagata aaaatatggc ttctgtaata ggaaaatatg
    28441 atgcagaagt gtgtataatg cataatcaag atgggacaaa ctatgacaaa gatataatgg
    28501 agtcaataaa agactttttt aaggtaagta tagaaatggc aatgagttgt ggtgtaaaaa
    28561 aagaaaaaat agtacttgac ccaggtgtag gatttggcaa ggattttgaa caaaatattg
    28621 aagtattgag aagattaaat gaattaaaag atttagggta tcctattttg cttgggacat
    28681 ccagaaaatc agtaatagga aaagtacttc cagttgagcc taaaaaaaga ttagaaggta
    28741 caatagctac tactgttctt ggtataagag atggcgtaga tatagttaga gtacatgatg
    28801 tttatgaaaa tttaatggca gctagaatga ctgatgcaat atatagaaag taggtataat
    28861 ataatggata aaatattact tagtaatcta ggattctatg gatatcatgg tgttcttaaa
    28921 gaagaaaatt ttctaggtca gaaattcttt gtcgacatgg aactttatat tgattcaaga
    28981 gaagcaggtc ttagtgacga tataaataaa tctgtaagct atgccgaggt ttacaatgtt
    29041 gttaaagata ttactgaaaa taaacaattt aatcttttgg aggctttagc ggaaaatatt
    29101 gctgaagaag tcttgaataa atttatactt ataaatggag ttatggtcag agttagaaag
    29161 cctgaggctc ctgtaaatgg tatttatgac tattttggtg ttgagataag gagaacaaga
    29221 gatgaataag gcttatttgg gaataggtac taacatgggt gatagatttg ataatttatc
    29281 aagagcatgt gaacttttga aaaatagtga ttctatatat aaagtaaaag aatcaagctt
    29341 atacgaaaca aaaccatggg gatatacaga acaagcagat tttctaaata tgtgtgtaga
    29401 aatagaaaca gagtttgaac cttatgagtt gttagaatat tgtcaagaaa tagagagaga
    29461 actacacaga gagagaatag ttcattgggg accaagaact atagatgtag acgtactatt
    29521 ttttaatgat gtagtttcca ctgatgaaag actaacaata cctcatccaa ggattcaaga
    29581 tagagctttc gttttgatac cacttatgga tttaaatgaa gaacttataa taaatgaaaa
    29641 aacaataaaa gaacatttaa acctcctatc tgctgaagaa agagaagaag taaaggagct
    29701 tgtaggttat gaaagaaaac ctatttaata ataattcaaa gatagagata aatacaaata
    29761 acaaaaagat aactgtaaaa aaagataaac ttataatagc tggaccatgt gcaatagaga
    29821 gttatgagca acttttggag actgctaaat ttgtaaaaag tcagggagca aatattttaa
    29881 gaggtggagc atacaagcca agaacatctc caaattcatt tcaaggatta aaaaaagaag
    29941 gtcttgaaat attaaaagcg gttaaagacg aagttggtat ggctgttatt acagaactta
    30001 tggatgtaag agatatggac gaactgtata gtataagtga tataattcaa attgggtcaa
    30061 gaaatatgca aaactttact cttttaagtg aagtgggaaa acaaaataaa cctgttatgt
    30121 taaaaagagg aatagcttcc acaattactg aatggatagg tgcttctgaa tatatagcaa
    30181 tagaaggaaa tagtaatatt ataatgtgtg agagaggtat aagaacgtat aatgattata
    30241 ctagaaatac attagattta gctgcagtac ccattattca aaaggaaaca ggtcttcctg
    30301 ttgtggtaga cccaagccat gccactggag ttagatattt ggtaaaacct atgtcactgg
    30361 catcttttgc atgtggagca gatggaataa tggtagaagt acatcctgac ccagaaaatg
    30421 ctttgtcaga tggtgctcaa tctctttgct ttaatgaatt tgaagattta atgaaatcaa
    30481 ttaataatta ttaaaagagg caagagcctc ttttttattc gtaatcgttg ctaaggtaaa
    30541 tttataagtt ataatttgat agttagatta tttaatataa ataaagattt aatattgata
    30601 gaattaatat aaaaaataaa aattttttga ttaataagaa ggatatcaat aaaaaaagtc
    30661 gaattgtgta tattgagtga ttgacaaatt tagaatatag tgtagaatag aaatgttaac
    30721 acaaaaaatt acaccagaag gtgataatat gaatgatata aaagacatta ttgaagaaat
    30781 aaaagctaga tgcgatatag caagtataat atctgaatat atgaatataa aacaatctgg
    30841 agctaattac aaaggattat gtccatttca tggcgaaaag acaccttctt tttatataaa
    30901 tacgtcaaaa cagatttata agtgttttgg ttgtggtgaa ggtggagacg taataaattt
    30961 tgtcatgaaa atggaaaatt tggattttat ggatgctgta aaaattttag caaataaatg
    31021 cggtatagaa attaacacta acatgaatga agaaactagg attaaaatag aaaaatctaa
    31081 gaaatttcaa gatattcata cggaagcagc tagattttat ctttcaaact tattgggaag
    31141 caaaaatctt gggtatgaat atttaagaat aagaggacta gatgataaaa ttattaagaa
    31201 atttggttta ggattttctt tggattcatg gaattcactt atgaatactc taatcagtaa
    31261 aggatataaa aaacaagact tattggagtg cggtctaatt gcaaaaaata gagatggaac
    31321 taattgctat gataaattta gaaatagagt tatgtttcct atattcgatt atagaggaaa
    31381 tataatagga tttgggggaa gagtattaga cgattctttg ccaaaatact taaattctcc
    31441 agacacgtta atttttaata aaaaacagaa cttatacgga ttaaattttg ctagaaaaaa
    31501 tttagaaagt aaaactatag tcttagttga aggatatatg gatttaattt cgctttatca
    31561 gtatggtata aaaaatgtag tagcaaccct tggaacagcc ttgactgaac aacaaggcat
    31621 tttaataaag agatatgcgg acactgctat tatatcttat gattctgatg aagcaggaat
    31681 aaaagcgact ttaagagcta tagatatact tactaaatta ggtatcaatg taaaagtgtt
    31741 agacttaaaa gatgctaaag acccagatga atttgtaaga aaatacggac ttagtgatta
    31801 taaaaaagct atggatgttt ctactcacta cataaaatat aaaatagatc atcttaaaaa
    31861 agaatttaac attcaaaaag atgaagaacg agtgaagttc gcaaaagaag cttctaaaat
    31921 tataaaacaa ttgacaagtc ctgttgaaat tgatttttat acaaagtatt taagcaatca
    31981 aatagatatt aatgtcgagt ctataaagag agaagtttat ggtaaaaatt acaataagcc
    32041 atataataac aaaaatcaaa agaaaataga agaaaaagta attgaaaaag tcgaagtcag
    32101 acaagacgga aaacaattag ttgaagaaac tctaataaaa ataatgttag aagacaaaaa
    32161 aattagagaa atagcgttac ttaaagtaga agaaagtgat tttttattga aagaaagtaa
    32221 agaaatttta aattacatga ttaaaaatca agaattggac aaaataacta ttgacaaatt
    32281 gaaaagttta aacatatccg aagaatactt aaaagaatta aattcaattt ctttgaatag
    32341 tataaactta gagaacacaa aagaaataga aggaataata acaaatatca gaaaaaactc
    32401 attagaagag caaattaata gcttgttgag agaacaacaa gaattagaaa ataataatga
    32461 tatgaaagag gtagatggta gagttatgga aattgcttta aaaatagttg agataaataa
    32521 aattttaaag agcttgtaga gaaaggaggt ttgagtgtgg aaaataaatc gaataaaaaa
    32581 gagttaaaaa aggttactgc taagacatta atagaaaaag gaaaaaagca aggttctctg
    32641 acacttgcag agataatgga agctttttcg gagactgaac ttgataagga tcaagtagaa
    32701 aatctttatg agactttagg aaatttggga atagaaataa cagaaacaaa aaactataaa
    32761 gctgatatag atttttcggt tgctgatgac gatttaagta taggccactt agatgaagat
    32821 gcagaggcaa tttcacatga tgactcttct gcaatagaaa tagaaactgt ggatttatct
    32881 ttaccaaaag ggataagtat agatgaccct gtaagaatgt acttaaaaga aataggaaaa
    32941 attcctctac ttaaaccaca tgaagaagta gaatttgcta gaaggatgca cgaaggtgat
    33001 gagatagcaa aacaaaggtt agttgaagca aacttaagac tagttgtaag tatagcaaaa
    33061 agatatgtag gaagaggtat gcttttcttg gatttaatac aagagggaaa tttaggtctt
    33121 ataaaagcag tcgaaaaatt tgactataca aaaggatata agtttagtac ttatgcaaca
    33181 tggtggataa gacaagctat aacacgtgct attgcagacc aagctagaac tataagaata
    33241 ccagttcata tggtagagac tataaataag ctaataagag tatcaagaca actacttcaa
    33301 gaacttggaa gagatccaaa accagaagaa attgcaaaag aaatggaaat gacagaagat
    33361 aaagtaagag aaataatgaa aatagctcaa gaccctgtgt ctttagaaac accaatagga
    33421 gaagaagaag atagccattt aggtgacttt attccagatg atgatgctcc agccccagca
    33481 gaggcagcag catattcttt attaaaagaa caaatagaag atgtacttgg ttcattaaat
    33541 gatagagaac aaaaagtatt aaagcttaga tttggtcttg aggatggtag agccagaact
    33601 cttgaggaag ttggaaaaga gtttgatgta actagagaga gaataagaca gatagaagca
    33661 aaagcactaa gaaaactaag acatccaagt agatcaaaaa aacttagaga ttatttagac
    33721 taaaaagata catcattgat gtttttgtga taaatgccat gagataagtg atatctcatg
    33781 gctattttgt agtaaagatt taattttatt tttgtagtta tttcagtcta atatctataa
    33841 cttggattaa atcctataaa taaagggaat tgaaaggaga attatatttg aaattaacag
    33901 atagattatt gaaaatagca tcattagtgt cagatggtaa aaaaatagct gatataggga
    33961 cggaccacgg atacatacca gtatatttgt taaaagaggg aaaagtacca tttgcagtgc
    34021 tagcggatgt taataaagga cctcttgaca atgcacataa agaagtcata cagaacaatc
    34081 ttttagataa agttgattta cgattgggtt ctggtataga gatacttgaa ataggggaag
    34141 tagaagaagt gataattgct ggtatgggtg gaatcttgat aagtgagctt ctcgaggcta
    34201 aaaaagaagt agcacataat gtagaaaaat taatattaca gccaatgcaa gcacaagaag
    34261 aacttagata ttacctttta aacaacggat atgaaatttt agaagaagtt ttagtgagag
    34321 aagattttag aatatatgag ataattgttg ccaaatatac aggtaaaaat actataatag
    34381 aagatgagat atattatgag gttggaataa agcttttaga aaacaaagat tctcttttta
    34441 atgattttat agaaaaaaag ataaagacat atagttctat agttaataaa ttagaaggga
    34501 aaaatgggga agctatagat aagaagagaa aggaaagcga agttgcaata aaaaaacttg
    34561 aaaatttaat aaaataaata ggaggtatat atgctattaa agagtctaac tagaaaaatt
    34621 gaaaaaaagt atcctctaaa cttagcagag gattgggata atgtaggtct tatagttgga
    34681 gattttgata tggatgtaaa aaaggtattg gtatctttag aagctaatga agatgttatt
    34741 gatgaagcta tatccaaaaa tatagattta attgtgacac atcatccttt tatatttggg
    34801 aaaattaata aaattaatag tggagattta aaaggcagac ttatccaaaa acttattaaa
    34861 aatgatatat cactttattc tatgcatacg aattttgata tagcatttga tggtctaaat
    34921 gactatttta tggaaattat ggaatttggt aattcaaagg ttttagatat aactaaaagc
    34981 gaaaatttgt ataaattagc agtatacgtt ccacataact attcagatga acttagaaag
    35041 gttttaagta attcaggagc aggtcatata gggaattata gtgattgtac gttttcaata
    35101 gaaggtgaag gacaatttaa gccattagaa ggctctaatc cgtttcttgg aagtgtaaat
    35161 gatatagaaa ctgtaaatga agttaaaatt gaaactgtag taccacagaa gcttttaggt
    35221 ggggtaataa gttctatgtt agatgcacat ccttatgaag aagttgcata tgatttgtac
    35281 aagttagaaa ataaaggtga gatatttgga ctaggtagaa taagtaaact tgataaaagt
    35341 atgacattag aaagtctttc aaggaaaata aaagagaaat taaatatgaa acacataaga
    35401 gtagttggaa accttagtac agacataacg aaagtagccg ttgtaacagg agctggttca
    35461 gaatttgtaa aaaaagctaa gagacaaggt gcagaagtac taattacggg tgatgtaaag
    35521 tatcatgagg cacaagatgc acttgatatt ggaatgtgta tagtggattg tggtcatttt
    35581 gatacagaag atatattcaa aaatgttatg aagagatttt tagatgaatt tagtgaaatt
    35641 gaagtaataa aaagtaatgt atatttgaat ccatttagta ctatttagca gtgtttgata
    35701 ataaaaatac tatataaaaa ataaatttga gtttaattag ggggataatg atgaaagtag
    35761 cagtaatttt tcatagtgtt tgtgggagta catatttatt agctagagaa tataaagaag
    35821 cgcttgaaga aatgaatatc gaggtagata tatttagagt aagtgacgaa gttgcaaaaa
    35881 cacttcctca atattatttg ataaattcca aagaatacaa agacgaattt gaaagtataa
    35941 atgtaattaa atctggaaaa gaaatattag attatgatgc tatatttatg ggttcaccaa
    36001 catattatgg aaatgtatct ggaccaatga agatgtttat ggatagtttt tctgatattt
    36061 gggtaggtac tcctttgagt ggtaaaatat ttggttgttt tgctacagca ggttcacaac
    36121 atggaggagg agaattagct ttgcaagcta tgaatatatt tgcccaacat atgggaatga
    36181 ctcttttgtc agttccatgt tcagtaagag gaggttatcc agcgtatgga atacttcata
    36241 tagcaggaga taattctgat ataagaccaa atgatgatgc caagataggt ataagagatt
    36301 acctaaaaag acttaacata taagaatatc atataaaaat acctaaaagc gactgtatat
    36361 acttttaggt atttttactt aattataaac gtgaagcttt accagaaata tggtcaattt
    36421 ctattttaac aacagttgtt aagttcatat ccttctctat gtacttcatg ccttcttcaa
    36481 agaatccttt agaatatttt tttattattt ctacaagagc atttttcttt tcttcatttt
    36541 ctacagtgca agcttttcca aaaactatag cactagaata agtagtacta aatttatctg
    36601 gaataacact ttcattagca actactaaaa aagaaacttt gttgtttttc gatatattat
    36661 ctaacttatg cccatttctt gcacaatgaa agtaaataga atcattaaag tatacataat
    36721 ttacagcaac tccataagga taaccatttt cagatatagt ggaaaaagta ccaaattcac
    36781 cattttttaa tacttcaaca gtatcttctt ttgtcatttc tctttttttc aatctcattt
    36841 ctttaaacat aatatcatcc tttctaattt tgatgcttaa attaaatttt agcacattta
    36901 taaagattat gctatgtttt ttacaaattt taagaaaaaa atatagttta agataaaagg
    36961 tagtgaaaga gaagctattt ggaggtttat ttatgatatt attgttagag tagtataaac
    37021 aatactaata aatttaatag ctggtttctt gttatttaag atagtaagca tgtctataag
    37081 aaggggaagt tatcgagggt agaagttgtt atttataact atattataaa gaaaatatta
    37141 ttatataaaa taataaatag taaagaggta tcaagttgaa tataatactt aatacctctt
    37201 caaaattaag ttataaaagt gttgtgggaa ttgtcctata catggtaagg agataataaa
    37261 gaatgtatct ttaaagtctc aactaaacag caaaatttat ccaacaaaca tacaataaat
    37321 gcttctttgt atctaggtgg cttaacagta agtggccaca tgtgttttaa aattatgtcc
    37381 gtttcttttt catttatttg aaaaaataaa gttgcatttt ttaatgcttc tctagggtga
    37441 gtaaatccat gtaatccttt tctatcacct ttgtaatgcc aatcataaag aaaaaagtcg
    37501 tgtagtaaag ctcctctaat aacactttga atatcaacac ttaagtgcaa ttttttacat
    37561 aatagataac tataatatgc tactgacaaa ctatgttcaa gacatgaaac attgccatga
    37621 tgaggaaatg ttctcataag ttgaactttt tcagaagaaa gtatctcaga agcacattca
    37681 ttaaaatatt tgatttcagc ttttgacaac attatgaaaa tctccattct tagtaatata
    37741 taaatactat acttcaacga taatatgaaa tatttcttat cctacaaaat ataataccca
    37801 ataatacaag taagatacct tttatggtat tttaaagtaa gagagatttt attaggctaa
    37861 acaatgaaaa aattaaaagt aaagctatag caatatatag tatttttcca agaggattgc
    37921 caaaatttat agtccatcca ataccaaacc ttttattaac cattaatgat gggtcatttg
    37981 gattattata catgatgcct gctatccaat acttctcatc ttcttctgga gaataagatg
    38041 agttgaattt taaatttggg gaattaatat atgttgctat taaataaatt ataactaaaa
    38101 gcgttgtaat attcatagca gttgatagtt ttgtttttat tgataaaaga gtagtaaaaa
    38161 actgtgttgt cattataatc atcaatatta aaaacaaata accaatttta tttaaatatt
    38221 taatgttttc aactctactt tcttcaattc tatttgtatc tatcttaatc cttgacttta
    38281 tggatccaat ggaagttata tataataaaa ccattaaaca aaagtcagta ccgattaatt
    38341 taaatacatt aagaaatgat ttatctataa aaacatcagg agacccattt atgttccaat
    38401 gagtggcaat tgagtctggc aattgattat aattaaatgc tataaaaata ctcattccca
    38461 aagtaaatag tatcggtatt aaatagatta ttttaaattt ttttattatt ttatttttct
    38521 tatttataaa atccatatct actattaatt ttgaatctat atctatatga cctaatttag
    38581 aataaatttc tgatttaagc ttcttagctt ttttatgtgt atgaatatat agtatacttt
    38641 catataataa aaaacctata ataccaataa aataggaaag ttccaattta ttaaatgtaa
    38701 agaccagaat aaaaactaat ataaagtcta taataaaacc taaagataat agttttttaa
    38761 attctttatc taactttata aagtaattat aatttttatg ctcttggtct atagaaactc
    38821 catagatgtg ttttttacct acaaaatagg gcataaaata aaatgtagca tacatcaaaa
    38881 ttagaactgg aaatattgta aatagacatt caatcttact cataatattt cacctcatac
    38941 ttgtcaaata aacttgaact tagtgacaaa aattcttctt tactaatacc tgataaataa
    39001 gattttgcca ccaataattc caagtcaagt ttattttttt gacgagttgt attatctatt
    39061 ttcattggta gttgagctac aatagcacct tttcttctat caatatttaa gaatccctct
    39121 tcttttaatt cattgtagga tttgttcact gtatgtaaat ttacaccaat attttcagcc
    39181 atacttctta cagatggaag tgattcattt attttcaatt ctcctgaagc tatagcctta
    39241 gttatttctt ctttaatttg tacataaata gatttatcac tattaaaatc taaatttaaa
    39301 accataaaaa acctccatta tgctttataa atgaaaacga ttgttatata catagtataa
    39361 cataaaaaat aataacaaac acaaaatata agtttatatt ttgtaaaaat tgtaatttaa
    39421 aaaataacat aatttttatt aaaataatac agatactatt tatagataga aataaaaatt
    39481 agaaaggaga aaaaatatgt taacatattt aatgaaaaga caaatgcata aaaaagatga
    39541 tggaatgagt aaaccaatga tgattacagg agctgtaatt acaggtgtag gagcatatgc
    39601 tgtatataaa atggtaaaaa gccataaatc taattctcaa atggtcaata caaattatta
    39661 tggaaatagc gattatgatg attataaata tgacgaattt gattatgact gtgattgtag
    39721 agacacaaaa caagattatg aatatgaaga attaaaaaaa aagtaaaaga atttaattca
    39781 agaagaataa attgtgaaaa ttgtccacat gtttctcaag aagaaatgat gcaatatgta
    39841 aatagtgtta aagatgtaaa ggaagagatt gatttacatg aagatgctaa aaaagataat
    39901 atttacaaag aagatgaata caaaaaatat gaagaagaat aagtaatatt ttgtataact
    39961 gaacaaatag taatgtcaaa ttatttaata tctagtttta atatgtaagt tgtaacttaa
    40021 cttgaaagaa tcagtatcat tctttagata gttaagtaac aacttacatt ttatttttat
    40081 aaattttaat agttctttgt gactattttg taattttaga aatattttag tatccagggt
    40141 aattgtgtat aataaaactt gtatagatag atagtatgaa attaagcaga tagtttaaaa
    40201 ttaaaatgaa aagaaaggaa atagttatgt taaagttgtt gatagttgaa gatgatagta
    40261 ctatatcttt tggaataaag tatgctttag aacaagaagg atttagtata gatatatcaa
    40321 aggatttatc tagtggaaaa gaaatgattt cttctaatga atatagcatg atactgttgg
    40381 atgttacatt gccagatgga actggttatg agctttgtca atatataaga ggattctctc
    40441 aggttcctat aatattcttg actgcttgtg atgaagaagt aaatattgta atgggtctcg
    40501 atataggtgg agatgattat ataacaaaac cttttagaat tagagaatta atatccagaa
    40561 taaaagctgt tttaagaaga aaaggtaata cttcagaaga aaataagaaa atacttaaat
    40621 ttggagattt aagtatatat acattagaag ctagagtata taaaaatgat aaagaaatat
    40681 ttctgacatc agttgaatat aaacttcttt taattcttat acagaataaa aatactgttt
    40741 taagtagaac tcaaatattg gaaaaacttt gggatgtaac ttatgatttt gtaaacgaca
    40801 atactctaac tgtttatata aaaaggctta gagaaaaaat aggtgatgat ttatgtgaac
    40861 caatctacat agaaactgta agggggattg gttataaatg gataggaagt gaaaatagtg
    40921 tttctatata atccagaagt gaagaagttt ttaagtaagt atgttacttt gatgtttatt
    40981 gttatcataa tatctattgg atttagcata atcaacgtgt ccttaactaa agagatggtt
    41041 gtaagaaata atcaggctat aataggaact ttatcaagca agtatccaaa cttggaaagt
    41101 cagatagtag acataataac tcaagggaag tctatggaga acatagatta tggtcagaaa
    41161 atattaatta agtataatta tgataaatct attaggataa actctgagcc tattatttct
    41221 aaattagttt tagagactat caaaataaat ataatattag tatgtataat atttattttg
    41281 atatttgtgt tggttgttag atattttaag tcaatttaca atgatttatc agatatgact
    41341 aagtatgttt actatagttc agagggtaag ccatttgata tgaaaaacaa gaatcaagaa
    41401 gggcagatag gtcttctaaa aactgaactc ttaaagatga caacaattct taatgaaaag
    41461 gttgaacttt taaaaagaga aaagatattt ttaaacaata caatttccga tatatcacat
    41521 caattaaaaa ctcctatgac ttctttaata atgttgaatg atttattata taatgatgta
    41581 ccatacgaag taaaaatcga ttttttaaat aagataaaaa atcaattaaa tagaatggat
    41641 tggttgatta aaagcatgct taaattatca aaggtagaag caaaagtgat aaattttaaa
    41701 aaagataagg taaaatttag tgaactcata catagagcaa tgctatctat gaaaatacct
    41761 atggagatta aaaatcaaaa actgactata gagggaaatg ataatgtatc ttatattggt
    41821 gatattgatt ggtccgtgga agcattagtg aatataatca aaaattgtgt agaacatact
    41881 cctgaatttg gaaatataat tataacctat aaagaaaatc cattattttc tgaactaata
    41941 ataaaagatg agggtgaagg tatagataaa aaagatatac cacatgtatt taaacgattc
    42001 tatagaggaa gaagtagttc aaaagaagat agtgtaggaa taggtcttgc aatgtcaaaa
    42061 tctataatag aaagccaaaa tggcgatata tatgtaaaca gcgaaaaagg tcagggtaca
    42121 gaatttcata taatatttca taaaatgtat gatagtgact aatctgtaat atttaattca
    42181 ctttatagta atagccaaaa cctaatatat gaagtataga atataaatat tggaggtagt
    42241 tatggaaatt ttaagagtag aaaatctaac taaaagttat ggtaaaaatg aaacaaaagt
    42301 tgatgctata aaaaatgtaa gtttatcagt tgaaaaagga acgtttatag caataacagg
    42361 tccaagtgga agtggtaaaa gtaccttatt acatttatta ggaggagttg acagaccaac
    42421 tagtggtaag gtttatataa atgatgttga tatatataat ttaaaaacta aggatttggc
    42481 gatatttaga agaagaaata taggtcttat atatcaattt tacaacttaa ttccagtctt
    42541 aaatgtgaag gaaaacatat tgcttccagc agagcttgac aatagagata ttgatagaga
    42601 atattttgat gatttaatga aaacattagg tctaattgat agagaaaagc accttccaaa
    42661 ccaattatca ggaggtcaac aacagagaac ctctatagga cgtgcattaa ttaatagacc
    42721 ttctataata ttagcagatg agccaacagg taatttagat agtaaaaatt caaaggaaat
    42781 attagaattg ttaaaattgt cagtaaaaaa atacaaccag acactaataa tgataactca
    42841 tgacaaaaat atggcacttc aagctgatag aattataagc atagaagatg gaatcataaa
    42901 aagtgatgag gtgatgtaga tgaatttata tacattacta accattagat acttaaaaca
    42961 aaataaaaga agaaccattg taaccataat cggtataata ctttcaacag ctttaatttg
    43021 tggtatagga aatatatttg aaagttttat ggattatcaa atgagagaaa ctataaagaa
    43081 tgatggaagt ttccatgtta cattttatga tgtaaataga aaaaatgtgg aatatgtaac
    43141 gaaatcagct gaaatagaaa aacatgcatt taccaaacaa cttggatatt ctaaattaga
    43201 aaatagtgaa aatgctatat taagtataaa gcaatatgat aaaaatgcaa taaatggata
    43261 taaagtttct ataaaagaag gtagatttcc agctaaggaa ggagaaattg ttctaagtga
    43321 aagtattatt aatcttatag atgataagtt aaaaatagga gataagataa ctttaaaagt
    43381 aggggatatg tttgactcaa gcaagaaaaa gattgatagt atgacatttc atgaaggtga
    43441 ttatatagca aatgaaaaag atagaacttt taaagttgtt ggtataattg aaaaacctgg
    43501 gtttgaacgt tataatggaa ttgcaacagc tatagcctac tttaatccta tgaataatta
    43561 caatgataca attaatgtat cagtagcagt aagaaatcct aaagatgtgt ataagataag
    43621 taataaaata agtaaaaata tatattcaaa taaagataaa gaagcagttt ctgatatgat
    43681 aaaatacaat gaacatttat tgaggttaca aggtgctagt aagtattcaa atataaataa
    43741 ctctataaag tctataatta tagcagtaac tattttagta atcatatgta caattgctac
    43801 agtgtacaat tcctttagta tttctataaa tgaacgaaaa aaacagtttg ggatattaaa
    43861 ttctataggt gctacatcta gtcaaattaa aagattagtt tttatagaag gtattataat
    43921 tagtttaatt ggtataccaa taggactaat atctggaaca gtagctatag acctattgtt
    43981 taaaattata aataagtatt ttactgaatc ggttgttact aagatgagtt tgcagatagt
    44041 gtataatcca ataataacta ttgttagtat aataatagtt ttgcttacaa tctttatatc
    44101 aattctacta cctgcaatat cggcttctaa tatttctcac cttaatgtga ttaaaaatac
    44161 tggagagtat aaagtgggca aaatcaaaag ctcaagactt attaaaatga tttttaaaac
    44221 tgaaggagta cttgcatata aaaatattag gagaaataga aaaaaattta taataactct
    44281 tttttcttta atggtaagta taataatatt tatatctttt agtggcttta cattactttt
    44341 actaagaggt gaagaaatta gaaattctca aagaaaatat gatttgtatt taactgcaaa
    44401 gaacacagct aggtctgtag aggatactat aagtgaacta gaagatattg atgggataaa
    44461 aaactttgaa ttggcaacag gacaatatgt gtctataaga gtaagtgaaa ataaaataaa
    44521 taaatccaag gaagatttaa tcaggaaata ttatcaaaaa tataagattg gagatagtta
    44581 tgaatatgat ttcagtaata atgaattaat ttttccagga gattttgcag taaaaaacaa
    44641 aataaataac ttagtacagg gctcttttaa taaagagaga gcaattgaag aaaatggtgt
    44701 tatattagtt agaaaaagtg catttgaaga aaatgggaag aaaggtgtag tagaacttac
    44761 caactataaa gtaggagata cagtaaattg tgaatatctt gatgaaaatg ccttaagtaa
    44821 aaaagtaaag gtcaagattc ttgctataac tgacgaagaa cgtctaggtc ttggttatca
    44881 aaatatgggg ttacaattta taacttatga tgaagttgct aaaaatttaa atttaaaatt
    44941 aagtaaaagc cttatattta ttgattcagg aggaagcatt aagactaaga aggaaataga
    45001 agctttggca aataaaaata attttaactt ttatgatgaa agtgcaagca atgaaagtga
    45061 aaaacaagat ttaaaggtta ttaaaatatt tgtatatggt tttatagtag tgatatcttt
    45121 agtgagtgtt acaaatattc taaatacagt aagtacaagt ataaatctaa gaaaaagaga
    45181 acttgcagta atacaatcta taggggttac tcctaaagga tttaggaaaa tgatatattt
    45241 agaaagtttt atatatggta tactgtcatt gttgtttgga ataccaatta gtattggtat
    45301 aattctaatt atgaataaac ttatttcagg tgtaattgaa ttttctccga ttataccgtg
    45361 gacagccata gttatctgta tagtaagtgt ttttataata acttttatag cagggtatat
    45421 accaatgagt aaattaaata aagaaaatat aatagataat ataagaagag aaagtatata
    45481 gatataaaaa taattcttaa ctatataaaa tattattaaa atcatttaag tggtataaaa
    45541 tattattata tcacttaaat gattttttat ttatataaga aatctaataa taaattacta
    45601 gaggtattta actatttaga aagaattggt attatataaa gtaagagaat atataataag
    45661 aaagaatatt tgattttatt ggaaaacaaa agaaaaagaa ggtgattact atttctcata
    45721 taactgcgaa gaatatgtat aaaagtttag aggaaagaat aaacaagttt ccacaaggtg
    45781 ctccaccatc tgatacatta tacaaaatac taaatgtact atatacagaa caagaagcga
    45841 agttagttgc tcagcttcca ataaagcctt tcagagtaaa aacagcagct aaaatttgga
    45901 gtgtaagtga aagtgaagca tatagagtat tggataaact tgctagtaag gctctgatac
    45961 tagatataga agataataaa gggaagaaat atataatgcc tcctccaatg gctggttttt
    46021 ttgagtttgc catgatgaga acgagacacg atatagacca aaaacttttg gcagagcttt
    46081 actatcaata tatgaatgta gaagaagatt ttatcaaaga tttattttat tcaacagaaa
    46141 cgaagcttgg aagagtatac gttcaagagg aagttttaac taatgataat gaggttagta
    46201 ttttagatta tgaaagggct actcatataa ttgatgaatc aactcatata ggtataagta
    46261 tgtgttactg tagacataga atgcaacatg ttggaaaagc ttgtgatgct cctatggata
    46321 tatgtatgac gtttgacaat gtagcaaatt ctttaataaa taataaattt gcaagacgtg
    46381 tggataaaat agaatgtaaa gaactacttc atcaagcata tgaacataac ttagttcaat
    46441 gtggagaaaa tgttaggaaa ggggttacgt ttatatgtaa ttgctgtgga tgttgctgtg
    46501 aagcaatggt agcagctaaa agatttggaa atcttcatcc agtacagaca acaagtttta
    46561 taccaaatat aaaccatgaa aattgtgtaa agtgtggtaa atgtataact gcatgtccaa
    46621 tagatgcgat aagcaaggtt aaggaagatg gtaaagaata tataaaaatt gatgaggata
    46681 gatgtctagg ttgtggagtt tgtgttagaa attgccataa aaatagcatt atgttattaa
    46741 agagagatga aaaaataata actccagcaa attcagtaca tagagcagtt cttatggcaa
    46801 ttgaaaaagg gcagttacaa aatttaattt ttgataataa tgctctggct agtcatagag
    46861 ctatgggagc tatactgtca gctatattaa agcttgaacc agctaaaaag attttagcca
    46921 gtaaacaatt aaagtcagta tatttagata aattgttaag tatgaatgat aaatagaaac
    46981 taacttaaaa aaggcaaagt tttagactta tctatatctt aataaactta tcatttaata
    47041 tttaaattta atttttacac atataaaact tataacttaa ataaaaaaag gctatattca
    47101 attaatatta cttgaataca gcctttttta gttatgttaa gctatttctt ttatttgctt
    47161 aaatgagttt tcatttccat ttccaccaat ttgtgttaca gatttaaatc tcatagtttt
    47221 aaatagttct ttttgattat aatctagtga gtttcctact agcattacag gagatttagt
    47281 ttttccagcc aaaactccaa cagataatcc gtctattaaa tcatcttgtt tattcattcc
    47341 atttttagca acatataaat tgtctatctt agaatccttg taaaattctt ttattacttt
    47401 tgcattagtt tcatttctag tgctacctga tattctctgt ggattttgaa gtttactttc
    47461 tatattttta gaaactgtat attctccacc gattacataa gatttatcaa tctttttgtt
    47521 tttaaacaag ttatttattt cagttatatt actattttca ttagtaagta ttataggcat
    47581 atcattttga gctgatacag ctccaatgct tactgcatcg gctaatcctt tttcgccatt
    47641 aactactgaa atctttgata tattgctaat attgttcaat tctttagcaa tgttcataga
    47701 agtttgatat ctatcacttc ctgaaattct agatacagag atattgtttg attttatagt
    47761 atctaaagat ttttcattta tagaagtttc tccaccaact acatatacat ttttaacttt
    47821 taatctcttt aattcagcca aagttttttc atttatttga gagcttccag tcaataatat
    47881 aggagcgttt ttcttgtatg caaatggtgt tgcagccaaa gcatctgcaa ttgctgaatc
    47941 gtttattaaa actgcatttt cagcattttc ccaacctgct tgacttatct taatagaagt
    48001 ttcatatcta tcagttcctg taagtgcact tgcatttact tcatcttgat atgtaacctc
    48061 aaatctagca tttggacttc ctaagaaaac aattacgcct ccttttggca atctaactct
    48121 attgttcttt gtaactaaag aatagtttac aggaacacct ttatcatcat aaacagcaaa
    48181 agctgaattt tgaggaagat taacttctat ttttttattt gcgatatcgt caccaatctt
    48241 ataccatttt gcataaccat tggattctag ttcacaagta aaagattttt ctgctggcaa
    48301 attagttata gaatcttcac taacatacgt tagtgttcca aaagataaat actctgtacc
    48361 attttcttta tgaagtttaa tgtcggataa atcacgacct attacaccag gtatttctat
    48421 aaaagcattt gagttattct catccattat tttagtgtta actatataac ctggagtttc
    48481 atcagaaaga ctaaaacttg cttttacaga gccaaacata tagctttgag aagtatattt
    48541 ttcatccact agatagtaac ctttgccact tctttttttc cacacttctt tgacactatt
    48601 tgatatattg ttgtcatcta ctttttgagc tacatagtat aaacttgctg tctgacctaa
    48661 tccagggaca tcatcataag agctcatatt taaatatgtg atattatttt tttcttttac
    48721 aaattttaaa caactattac ctttttcaga tacaaatcta tcttgaccta tgtatacgta
    48781 tttatcttca ggtccatttt ctatataagg agaagatact gttaaagtac cattatcatt
    48841 aacatctact tttatcatat ttgaagaagc ataaagacca ctattttctt ttaaagagct
    48901 aggcatttta acttgttgtg gcttactaaa tgttttatca ggttttattt ctttaatttt
    48961 acctttttct tttaatgcag atagtaagat ttcttgtcca attatttcat taagttgact
    49021 acttccacca gaagaaagaa ctgcaactgc catattttca tctggaagaa ctataagatt
    49081 actgtggaat aataaagaat ctccaccttt tgtaagagct tttaaattat actgattaaa
    49141 tggataagtg ttgacacaat cccaccctaa accatatcca agtatagagt cttctccttc
    49201 aggccataga ccatttaaat attctttgtt ttccatagct tttactgaag caggagaaag
    49261 aataccattt gaatttttca taaatgtttg tgcaaatgta catagatttt ctgcacttga
    49321 atataaacca cctgcaccta tagcattaag gttatcttgt ggaactgcat cttcccaata
    49381 aggtacatat gcttttgcta gttttgaact atcaaaacta ttttctggag tttttgtatt
    49441 ttgtaagtta agtggattat ttatgtattt atcaagaaaa ttagtaaatg acataccaga
    49501 tactctttct actaaaattt cagctaaagt aaatccatca ttacaataaa cagaaaaagc
    49561 tcctggttta gcttttaatc tttgtttttg taattctttt aagaaattat catgaccata
    49621 agaatcattg tctgctaata aaatagtgtt tttaaaacta cttcccatca accctgatga
    49681 atgatttaat aacattcttg gagtgatttc cttgtatcta tcatcagcca ttttaaattc
    49741 aggtatatac tttacaacag gagtatctaa atttaatttt ccatcatcaa ccaacttcat
    49801 aacagctgtt gtagtaaaca ttttactgat agatgcgata ctatacatat tatccttatt
    49861 taagtttttg ttatcctgtt tgctgtaaac gccaccatta ccagaaatct caattttgcc
    49921 attatcaatc aaagcatatt gagcacttac agcaccatac tttgatagca aagtttggat
    49981 tttctcttta gcaagagctt tagtttttgt gtaagattcc gcgtttttac tataatcatg
    50041 taattctata tcagaacttt gatatttttc ggaattatct tttgtattat cagcaaaagt
    50101 aatatttgga gcagcagtaa ataacatact caatactaat gtactagata ataatttaga
    50161 gagcctttgc ttaaacatat tacattacct ctttcccttt ttaataattt ataattagca
    50221 taacattctt aattatttta atcaacagat tattatcaat aaaaaaagct tttataagta
    50281 tagcttttta tataacagag aaaaatataa aaataatatt tgttgttgta taaagaataa
    50341 agggtatagg taatatataa ataaaatgga ggaattttag acatatgtat aagaatataa
    50401 ataagaagca acttaaagaa atgatgaaaa atgaaaagga tgttttatta ttagatgtaa
    50461 gaactaaaga tgaatttaag gaatataaaa tagaaaatgc tataaatata tctttacaag
    50521 agttaatcaa taatatagat gaaatttacg attataaaga taagaaagta gtagtttatt
    50581 gcagaagtgg tcatagaagt gttactgcat gtaatttgtt agctgaagaa ggttttgagc
    50641 atttatataa tttaaactca ggaataattg attatatgag ttaaataatg taaattatac
    50701 attaaatact atgtatagct ctgcttatta agtaggaata tatctaaata tttataagat
    50761 aaatttagga aagaacgaat tttttcgttt ttttctatga cagtttttgt agtaaatatt
    50821 agtttttaag ttggaaaaag gtagtagctt tttagctact accttttaat attaacttag
    50881 aatattatta aaaaaatata tttaaagaca gaattaattt tttaatgacc agttaaatta
    50941 ctatcgccaa atttatcatt aaatggtttt ataactctat ttataggtcc ttttagaagt
    51001 acgtttaaac aaatagacag aatcataaat agtaatagaa ctagtatatc tttagttaga
    51061 tttggagcat atataccacc aactgcttcc cttgtagcac ctattgcata ggtaaatggc
    51121 attatagggt ttattctttg gaagaatgta ggtgtaactt gtatagggaa tgtaccacca
    51181 gaagaaccaa tctgaacgac taatagtact attgcaattg cttttcctac atttccaaat
    51241 acagaaacta aagaatacac tataaagtta aataccatac tagtaaacat aagcagaact
    51301 atcaataaaa ttggatgttt tgctgttact cctaacaata atatatctcc agttcctatt
    51361 ataaaacctt ggacaagagc tatacttaaa aaagtaaggc ctcttccaaa gtaaatttca
    51421 taaggtttat agtttccatg tacagatgta gacaatagtg ctgaaataag taatatacca
    51481 acccatgtag aaagaacact ataaaatggt gtcattgcag aaccataatt ttcaattgga
    51541 tataatttct ctgttttcac ctcaacaggt tcttttaaga aattagactt tgtcaatata
    51601 tcactttcaa gcaagctgac taattttttc atatcttcac cattactaat tttgcttaaa
    51661 gtatcaatca aatcatccaa cattcctttt gctaaaggaa gtctttctct tatcaatgat
    51721 atactttcct gtgcatttcc tgaaagtttt agagaagtag taagtatttc ttcaaccttt
    51781 ggaagttttg cttctgcctt atctaaaaca gtaattatat catttgctac ttttatactg
    51841 tttgcaaata tactatttat aggttttgat atttttgagt caaaattatt taaaatattt
    51901 aaattaattc ttccaatacc atttgataga cttaaaacat tattcaaagc acttatagat
    51961 ggttgttgtc cagatataac tttgttttta atatcattta atgttgatat agatgaatct
    52021 attttattgc tagagtcttc aagattatct atgacatcat ccaatctatt atttgatgta
    52081 agttggttta atttagttaa aaaatccacc aaagtatcat ttaaagattg caaattagaa
    52141 agtttttctg ataaattatc tatcaactta ggtacatctt cactgccact atttactgca
    52201 tcaattaaat ttaatgtaag agaagaagca gaagatgaaa ggtcaaccat taaatttaag
    52261 tcagacttaa taagaggaga aagttcgtct aaattatcat ttgtatcctc taagaatttt
    52321 tttaagtcac ttgataatag ttttgtatca ttcagagttt ttttaattgt aggtaaatca
    52381 gatttaatat ctttaactgt atcagataat tttgaagtag catcagaagc taaatcaaca
    52441 gtcttattta tatggtcaaa tctcttttga aggtcaataa gggaattttc aacttttgtc
    52501 aattttggaa gactgtgttc aattcctgca ccaactccgt ttgatatacc aagaacgatt
    52561 tcactaactg tttttactat agtctgatta acctgtagtt gtattgtaga agcaccttta
    52621 tctgtaatct ttggtgcaac tgcatttatt ttttcattaa cagtatagat aatttcgcct
    52681 tttttaacat catctgtaat taaagatgtc aaatcttttg taaaattttt tggaatctta
    52741 agtgatgcat aataagtacc ttttttaaca ccttcaagag cagttttttc atctacaaat
    52801 tgccaaccta aactcttgtt atctttgagt tgttcaatta attggtctcc tacattaatc
    52861 ttttgccctg ctatttcatt tcctttatct tcatttgtta ctgctacaag catattggca
    52921 gtgcttccat aagggtccca agatgccttt atattgaacc aagcatacag agatggtaaa
    52981 aaagcaagac ctaaaactat tacaagtacg gcataattgc cacgtatatc ttttaggtca
    53041 cttttaaaaa tttctaatat attttttaaa ccctttgaag gtcttaaatt cttgatatta
    53101 ttccagttta aaatttcttt taaactatta gtttttttca aatgtctcac ctcctaaaat
    53161 attttgataa agaaaaaagt tagatttata atcaatttta aattatatta ctttttacac
    53221 aaatagtaaa gttaaaattt tgtcttaat
//
LOCUS       DAGSRM010000018        51361 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.018, whole genome shotgun sequence.
ACCESSION   DAGSRM010000018 DAGSRM010000000
VERSION     DAGSRM010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 51361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51361
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.018"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            18..92
                     /locus_tag="MJE33_001171"
     tRNA            18..92
                     /locus_tag="MJE33_001171"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:50..52,aa:Gly,seq:gcc)
     gene            117..193
                     /locus_tag="MJE33_001172"
     tRNA            117..193
                     /locus_tag="MJE33_001172"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:151..153,aa:Arg,seq:tct)
     gene            203..278
                     /locus_tag="MJE33_001173"
     tRNA            203..278
                     /locus_tag="MJE33_001173"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:236..238,aa:Gln,seq:ttg)
     gene            287..362
                     /locus_tag="MJE33_001174"
     tRNA            287..362
                     /locus_tag="MJE33_001174"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:320..322,aa:Lys,seq:ttt)
     gene            365..453
                     /locus_tag="MJE33_001175"
     tRNA            365..453
                     /locus_tag="MJE33_001175"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:399..401,aa:Ser,seq:tga)
     gene            457..532
                     /locus_tag="MJE33_001176"
     tRNA            457..532
                     /locus_tag="MJE33_001176"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:490..492,aa:Phe,seq:gaa)
     gene            539..612
                     /locus_tag="MJE33_001177"
     tRNA            539..612
                     /locus_tag="MJE33_001177"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:573..575,aa:Met,seq:cat)
     gene            627..703
                     /locus_tag="MJE33_001178"
     tRNA            627..703
                     /locus_tag="MJE33_001178"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:661..663,aa:Ile,seq:cat)
     gene            721..797
                     /locus_tag="MJE33_001179"
     tRNA            721..797
                     /locus_tag="MJE33_001179"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:755..757,aa:His,seq:gtg)
     gene            806..881
                     /locus_tag="MJE33_001180"
     tRNA            806..881
                     /locus_tag="MJE33_001180"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:839..841,aa:Lys,seq:ttt)
     gene            889..962
                     /locus_tag="MJE33_001181"
     tRNA            889..962
                     /locus_tag="MJE33_001181"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:921..923,aa:Cys,seq:gca)
     gene            970..1046
                     /locus_tag="MJE33_001182"
     tRNA            970..1046
                     /locus_tag="MJE33_001182"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1004..1006,aa:Arg,seq:acg)
     gene            1059..1134
                     /locus_tag="MJE33_001183"
     tRNA            1059..1134
                     /locus_tag="MJE33_001183"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1092..1094,aa:Val,seq:tac)
     gene            1210..1623
                     /locus_tag="MJE33_001184"
     CDS             1210..1623
                     /locus_tag="MJE33_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647129.1"
                     /translation="MYIVIGNEIVDSEELKIIIDKNSEFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLINEQCNLASLSDEEKFDEYMTLSDELALDLEELMPKYTIINARAYKL
                     DEVDGIVKIILAVAYADLGHLKLSDVVKRLSRQVD"
     gene            1931..5362
                     /locus_tag="MJE33_001185"
     CDS             1931..5362
                     /locus_tag="MJE33_001185"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBY9647130.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHREEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLDDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKSEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTKESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNVKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYNKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPAEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            5604..7532
                     /locus_tag="MJE33_001186"
     CDS             5604..7532
                     /locus_tag="MJE33_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBY9647131.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISSYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGVLSKDKDVFNINKNVKEKIANIYTLRNSELVEIEKAKAGDIVVITKVNSLK
                     TGDTISADKDAEALEKIDFPKPQIYYAVTPKNKGDEEKVASVLNKLVEEDPTLHWYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGTADVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFTEEIFGGSVPKQYIPAVEKGLKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASAAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
     gene            7985..8449
                     /locus_tag="MJE33_001187"
     CDS             7985..8449
                     /locus_tag="MJE33_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBY9647132.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            8456..8950
                     /locus_tag="MJE33_001188"
     CDS             8456..8950
                     /locus_tag="MJE33_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HBY9647133.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            8966..9991
                     /locus_tag="MJE33_001189"
     CDS             8966..9991
                     /locus_tag="MJE33_001189"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HBY9647134.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLRAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            9978..12425
                     /locus_tag="MJE33_001190"
     CDS             9978..12425
                     /locus_tag="MJE33_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBY9647135.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNRVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            12605..13978
                     /gene="radA"
                     /locus_tag="MJE33_001191"
     CDS             12605..13978
                     /gene="radA"
                     /locus_tag="MJE33_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBY9647136.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSENLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDEDIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            13981..15051
                     /gene="disA"
                     /locus_tag="MJE33_001192"
     CDS             13981..15051
                     /gene="disA"
                     /locus_tag="MJE33_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA"
                     /protein_id="HBY9647137.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAVTNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            15140..16234
                     /locus_tag="MJE33_001193"
     CDS             15140..16234
                     /locus_tag="MJE33_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBY9647138.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            16363..18540
                     /locus_tag="MJE33_001194"
     CDS             16363..18540
                     /locus_tag="MJE33_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647139.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDISTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSDNGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KEAYKNNDVIFVELGDTYRLDLYKPNLNEKTYESMKDSIEKNIDIYLKNIFSMVEEND
                     TVYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWSIIVFSLVILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVVFVLGVFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILVLMINQIRYFKQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYVIVPMITLSINLTALKE"
     gene            complement(18726..19571)
                     /locus_tag="MJE33_001195"
     CDS             complement(18726..19571)
                     /locus_tag="MJE33_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY9647140.1"
                     /translation="MNRNNFSHEIIEPIDNLDVNFKLFDDSGSYVANHWHNSLEIIYI
                     TSGDLQINMEGYTYNLKANECMFINSGIIHSTRCIYQNTSILLQVPLSFLNKYIPDFK
                     NCYFDFKVNANDNNYKKNSSKVKLILENMREIKLSSPPAANLLFTSLLFELLFELYTN
                     FKISVGNKNMKKTVLDLSKFEPVLEYTKINYKMPISINKIAKVAHLQPEYFCRKFKQY
                     MGQTYLEYLNDVRISHIYKDLINTNDTLCSILEAHGFTNTKLFYRIFKEKFKCTPKHI
                     RKKST"
     gene            19788..20954
                     /locus_tag="MJE33_001196"
     CDS             19788..20954
                     /locus_tag="MJE33_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HBY9647141.1"
                     /translation="MYIKGVNLGGWLVLEKWMTSSLFEGTEAEDEYYLPRQLSREAYE
                     SRIKTHRSEYITERDFATIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFAWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGICSWSQNPEYVAFTLNVLERLAKRYGMRHELY
                     GIQILNEPITERMWNIMNVPNRFKAVDKEMARGSKPNSLEFLRDFYIKAYKVMRPYMR
                     EENVIVFHDAFELKAWKDFMREEEFKNVVLDTHQYLMLAEADGCEQSIDSYLKYIREN
                     YAKDILQMQKYFPVICGEWSLFNSYACGIDTNGGQSPLNGIESNIDKLSKDDKRELYR
                     KIAKAQLDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLGKCVAQEWYPIEY"
     gene            20976..22622
                     /locus_tag="MJE33_001197"
     CDS             20976..22622
                     /locus_tag="MJE33_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBY9647142.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVYGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYLHSSGFDASIFHDDDGKKWIASLDWETREGYEKPGVICL
                     VEYCTKKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHSVTMGR
                     AKNIWGPYEKDSMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETTSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMKWTKDNWLRMEDESNLAKEYVSESKLEEH
                     LVSSIPSFDDFDSNELGLQYYAPRISPLSFADVKSRPGYVRIRGQESRTSLNKVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRIPIKDIPIYLRLYIQGRKSYFEWSYDEKNYQRIGKVFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIVDESKDVD"
     gene            22719..24092
                     /locus_tag="MJE33_001198"
     CDS             22719..24092
                     /locus_tag="MJE33_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY9647143.1"
                     /translation="MNTEVNVKATTIKYEKISFFERISYGCGDLGCNIIYSAMSAFLL
                     FYYTNYADVSAAAVGSIMLVSRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     IAAVLLFSVPSNLGITSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQNQYERGVL
                     SIFRMILATCGTLLINGLTLPLVEYFGNNLSAWTKTFFVFGIASIMVFFITFTGTKER
                     VKAVKQDRNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFGPINMVSNISQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIVGQNHIG
                     VIFASAIKGIGSAGIASTMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGAVLSIGGYVGTSATQSDLAIVSIKVCFIYLPIFITILQIIIMSFYKLDKEYSTI
                     LNELNTK"
     gene            24537..25490
                     /locus_tag="MJE33_001199"
     CDS             24537..25490
                     /locus_tag="MJE33_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HBY9647144.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            25528..26490
                     /locus_tag="MJE33_001200"
     CDS             25528..26490
                     /locus_tag="MJE33_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HBY9647145.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            26515..27501
                     /locus_tag="MJE33_001201"
     CDS             26515..27501
                     /locus_tag="MJE33_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBY9647146.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGFVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEAAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEEAIKSLIVR"
     gene            27514..28560
                     /locus_tag="MJE33_001202"
     CDS             27514..28560
                     /locus_tag="MJE33_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HBY9647147.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYNGKIRKCDVEKLTAKETIVSTNTSKSSE
                     KKELKIENENSSMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKV
                     LRAKLIDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            28727..30457
                     /gene="lpdA"
                     /locus_tag="MJE33_001203"
     CDS             28727..30457
                     /gene="lpdA"
                     /locus_tag="MJE33_001203"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBY9647148.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDTV
                     KENQNIEVKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVDVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEEPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSMPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEV
                     DLGALPSCVYTIPEVAAVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
     gene            30875..33823
                     /locus_tag="MJE33_001204"
     CDS             30875..33823
                     /locus_tag="MJE33_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY9647149.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEVISSRLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKEIQDSGDL
                     KLDKESDDKKEEIINNRENKDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFAVESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADENKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETRLTHKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKLKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIIGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LSSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPVEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRDMSEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEATTIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGINIRTI
                     SRFDTLLSIEVIRRAVLPDLTIDEIVDEVKGIEPQVKEKVSKCNNTKVDFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKAKLNQINNLIDTNQIGETNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSDEIAIPHTDGIDVIRPAI
                     SIAILKKPIVWSKSKVKIILLLAVDQKCFNPLSTLLSFIETDEFKKIKNIKDRDFIRE
                     MILDGVNENN"
     gene            33801..34265
                     /locus_tag="MJE33_001205"
     CDS             33801..34265
                     /locus_tag="MJE33_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY9647150.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKESYIQ
                     AVVAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEIDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVDLSEESVSDLLNSKLKN"
     gene            34378..34680
                     /locus_tag="MJE33_001206"
     CDS             34378..34680
                     /locus_tag="MJE33_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY9647151.1"
                     /translation="MGVTNVAKKILVACGTGVCTSTIAINKLKKALQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIICTTQVSSSIKTPVINGLPFLTGVGMDKLINDVLEELEL"
     gene            34742..36106
                     /locus_tag="MJE33_001207"
     CDS             34742..36106
                     /locus_tag="MJE33_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425524.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBY9647152.1"
                     /translation="MEGLYMEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGL
                     TVGVGFIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALI
                     IPIGLVVNIVMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFAAVANMVIV
                     LVMADLTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQ
                     KKFGLFGEPLIMGSVIGVIIGIAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLP
                     VSEAAQEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVL
                     PFADLAVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFK
                     MPEGATMISSICDGANPLSWVFVKVMNYKVIGGVVFGVIALGMAIYNRNRIINENKKL
                     SIEE"
     gene            36141..36614
                     /locus_tag="MJE33_001208"
     CDS             36141..36614
                     /locus_tag="MJE33_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647153.1"
                     /translation="MKKYKYDSKRYLLSVTIPGFFMILILIYALFNNYTNFNLNIYSL
                     LIIVCTYSIFNTFISSSNPKKIIIDSKCIHFTSFMTTHKYELKDIEKIRIREFYNKKI
                     YLKINDGNLFKGRYWIRTNMFNNSIALYSYFIELEECLYPDSLKFRNKKFENKNI"
     gene            36786..37436
                     /locus_tag="MJE33_001209"
     CDS             36786..37436
                     /locus_tag="MJE33_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBY9647154.1"
                     /translation="MLLEKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLTPSDIVIVDVDGNIIDGKWKASVDLSDHLYIYKHREDINSIIHTHSTYSS
                     CFAILNEPIECASTTLANEVGGSVPVAKFSPPTSKKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            37610..38542
                     /locus_tag="MJE33_001210"
     CDS             37610..38542
                     /locus_tag="MJE33_001210"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBY9647155.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEESIFIG
                     KNQNPYQEVYHDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKDRQLHEIDFLEHT
                     KFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEED
                     IKSVYNIGEDKYKKIEWTYGKTPKFQVVLPFNNESKINIHVNRWKVSKFFISYDDNVI
                     NMDKLLDLNFFKEEIKESILENYPHYIDLINLIF"
     gene            38816..39529
                     /gene="ispD"
                     /locus_tag="MJE33_001211"
     CDS             38816..39529
                     /gene="ispD"
                     /locus_tag="MJE33_001211"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HBY9647156.1"
                     /translation="MYSVIIVAAGSGRRMNLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFKENIINKYNFKNIKIAYGGKERQDSIYNGLKKLDKNCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVKETPERNLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            39526..40011
                     /locus_tag="MJE33_001212"
     CDS             39526..40011
                     /locus_tag="MJE33_001212"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HBY9647157.1"
                     /translation="MRIGLGYDVHKLTEDRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    40106..40314
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            40378..42093
                     /locus_tag="MJE33_001213"
     CDS             40378..42093
                     /locus_tag="MJE33_001213"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBY9647158.1"
                     /translation="MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDVDDKGLDKSYQDMFDAYVSIFNRCGLENSPVQADSGAIGGSTSAEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNLASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDKCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIVPANMKNEEQISIAENIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLG"
     gene            42215..43660
                     /locus_tag="MJE33_001214"
     CDS             42215..43660
                     /locus_tag="MJE33_001214"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBY9647159.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKAKGVAVEVDDRDNYT
                     TGWKFNEWEMKGVPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
     gene            44099..45580
                     /locus_tag="MJE33_001215"
     CDS             44099..45580
                     /locus_tag="MJE33_001215"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBY9647160.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLIEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEVTEHASIFF
                     GDKIEVETEEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            45801..45956
                     /locus_tag="MJE33_001216"
     CDS             45801..45956
                     /locus_tag="MJE33_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647161.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            46104..47501
                     /locus_tag="MJE33_001217"
     CDS             46104..47501
                     /locus_tag="MJE33_001217"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBY9647162.1"
                     /translation="MKVYNTLTRTKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEDKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKTFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEVELVKELDDFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFAK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLDMG
                     IVLEDTRQGVKWKRI"
     gene            47486..47899
                     /locus_tag="MJE33_001218"
     CDS             47486..47899
                     /locus_tag="MJE33_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBY9647163.1"
                     /translation="MEKDIKMEKTELVTMSPLVLAYLGDTVYETYIREYLIRQNTQRK
                     VNDLHKLAIKYVKAKAQATIIHEIEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKH
                     ATGFEALVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            48005..48763
                     /locus_tag="MJE33_001219"
     CDS             48005..48763
                     /locus_tag="MJE33_001219"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HBY9647164.1"
                     /translation="MKVKLISHTPEPEKVIAMAAKLCYSPVGTDEIEKDLTDESIEKF
                     LNMLLSIGHGSILEHASFTFSIEGISRACSHQIVRHRIASFSQQSQRYVKLEQFEYII
                     PPEIEKIEKAKELFIDSMKKDQENYDKLVEILFENHYNDLIKNGKNEKTAKRQAEKKA
                     IEDARYVFPNACETKMVFTINARSLFNFFEHRCCERAQWEIRNLAVEMLREVKKVAPI
                     LFKKTGPSCVNGSCPEGTMTCGDIVQVREKFKAL"
     gene            48776..49513
                     /gene="rlmB"
                     /locus_tag="MJE33_001220"
     CDS             48776..49513
                     /gene="rlmB"
                     /locus_tag="MJE33_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBY9647165.1"
                     /translation="MASIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMAKDK
                     NIIIQYVDKHKLDEVSTSHSHQGVIAYASEYKYYELDELIDLAKNKDEDPFFIILDEI
                     TDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIVNTI
                     KRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMPMIG
                     NVTSLNASVAGGILLYEIFRQRLDKSK"
     gene            49516..50043
                     /locus_tag="MJE33_001221"
     CDS             49516..50043
                     /locus_tag="MJE33_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBY9647166.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDD
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DKETLSKLENIRRKR"
     gene            50108..50755
                     /gene="sigH"
                     /locus_tag="MJE33_001222"
     CDS             50108..50755
                     /gene="sigH"
                     /locus_tag="MJE33_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HBY9647167.1"
                     /translation="MLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIITKYRNF
                     VKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQIITAIK
                     TATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKNIESKM
                     NELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENRND"
     gene            50829..>51361
                     /locus_tag="MJE33_001223"
     CDS             50829..>51361
                     /locus_tag="MJE33_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBY9647168.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVCSATDGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLT
                     EYDFPGDDTPIVRGSAL"
BASE COUNT        20942 a         5483 c         9714 g        15222 t
ORIGIN      
        1 tttaatttaa taaacgagcg ggaatagttc agtggtagag cgcaaccttg ccaaggttga
       61 agtcgcgagt tcgaatctcg tttcccgctc caaattttat gtttatgttg taatgtgggt
      121 gcatagctca gctggataga gcaacgccct tctaaggcgt gtgtccgggg ttcgaatccc
      181 tgtgcgctca ccacatatat attggggatt agccaagtcg gtaaggcaca tgactttgac
      241 tcatgtatgc gtaggttcga gtcctgcatc cccagccaaa cagtatggtc tattagctca
      301 gtcggtagag cacctgactt ttaatcaggg tgtcccgcgt tcgagtcgcg gatagatcac
      361 caatggagag gtgtccgagt ggtttaagga gctggtcttg aaaaccagtg acttcgaaag
      421 gggccgtggg ttcgaatccc accctctccg ccaaatgccc agatagctca gtcggtagag
      481 caggggactg aaaatccccg tgtcggtggt tcgattccgc ctctgggcac caaaatgtgg
      541 cggtatagct tagttggcta gagcgttcgg ttcatacccg aaaggtcata ggttcgactc
      601 ctattaccgc tactaaaatt taatgtgggc cattagctca gttggttaga gcgcccggct
      661 cataaccggt aggtctgggg ttcgagtccc tgatggccca ccacattaaa ttaacttatg
      721 gtgggtatag ctcagttggt tagagcgcca gattgtggct ctggaggtcg tgagttcgac
      781 tctcattatc caccccaact tatatggtct attagctcag tcggtagagc acctgacttt
      841 taatcagggt gtcccgcgtt cgagtcgcgg atagatcacc aaaaaggcgg cgacatagcc
      901 aagtggtaag gcagtggact gcaactcctt gatccccagt tcgaatctgg gtgtcgcctc
      961 caataaaatg cgctcatagc tcaactggat agagtgtctg actacgaatc agaaggttag
     1021 gggttcgagt ccctttgggc gcaccatatg aatagcgagg gattatagct cagctgggag
     1081 agcacctgcc ttacaagcag ggggtcacag gttcgagccc tgttaatccc accagtgcaa
     1141 acctctagta aaactactag aggttttatt tttgattgaa aaagttttta tatttaggag
     1201 gtaaaaaatt tgtatatagt aataggaaat gaaatagttg atagtgaaga acttaaaata
     1261 attatagata aaaatagtga atttaaagtt gaaaaagatt taagtaaaag cacaaaaaga
     1321 gaagatgtaa tagcatatca gttaagtata gatttaaatt acctagattc tcttataaat
     1381 gagcaatgta atttagccag tctatctgat gaagagaaat ttgatgaata tatgactttg
     1441 tctgatgaac ttgcactgga tttagaagag ttaatgccaa aatatacaat aataaatgct
     1501 agagcatata aattagacga ggtagatgga attgtaaaaa taatactagc tgtagcgtat
     1561 gcagatttag ggcacttaaa actttctgat gtagtaaaga ggctatctag acaagtagat
     1621 taggatatga atatgaacta tattaaaaaa atgtataaaa tcttacgtaa agatgaaaat
     1681 aattatgtaa acatagaaaa aacagtataa aatcaagtca attgtagaaa ttttcacata
     1741 aattcatctg aaattctata aaaaatgcct taattttaat gaggtaaatc gagtttacac
     1801 agtctaaaaa ttacttatat ttataaattt atcttattaa ttaaggaact ttcttaaaca
     1861 tcttcaaaaa taaagtattt tagtctataa tctttaatat tacacaaaca ataaaggaga
     1921 agaatagagt atgcttaaaa agtttaataa aatactagtg gctaatagag gcgaaatagc
     1981 tataagaata tttagagcct gttcagaatt ggggataaaa agtgttggta tatacagtaa
     2041 ggaagataag tatggattgt ttagaacaaa agcggatgaa tcatatttga taggagaagg
     2101 taaagggcca atagatgcat atttagatat ggatggaata atagacctag caaagagaaa
     2161 aaaagttgat gctatacatc caggatatgg ttttctagcg gagaatgcag aatttgctcg
     2221 taaatgtgaa gagaatggaa ttacttttat aggaccatca tctaaggtaa tgaatatgat
     2281 gggggataag attaactcta aaaaaatcgc aaaagaagtt aatgttcaaa ctataccagg
     2341 agtagaaaaa gcaataagaa gtactgaaga agcaaaggaa gttgcaaaca aaataggata
     2401 tcctgttatg ataaaagcat ctaatggcgg tggcggtaga ggtatgagaa ttgtacatcg
     2461 cgaagaagac ttagagcttg aatatgaaac tgcttgtagt gaatcaagaa aagcttttgg
     2521 agaagatatt atatttatag aaaaatatat agctgatcca aaacatatag aagttcaaat
     2581 attaggtgat aattatggaa atatagttca cttatatgaa agagattgtt cagttcaaag
     2641 aagacatcaa aaaataatag aatatgcacc agctttttcg ttagatgata aagtaagaaa
     2701 agagatatgt gaagatgcag ttaagttatc aaaacatgtg ggttattcta atgcgggaac
     2761 attagaattt ttagtagatg caaatggtgg gcattatttt atagagatga acacaagagt
     2821 acaggttgaa catactgtaa cagagatggt tacaggaata gatatagttc aaagtcaaat
     2881 actaatagcc caaggttaca gcttagatag tgaagaaatt aatataaaat cacaagatga
     2941 tgtggaaata agaggttact ctatccaatg tagaataact acagaagacc ctaagaataa
     3001 attcatgcct gatacaggta aaattcaagt ttatagaact ggttcaggat ttggtataag
     3061 actagatgga ggaaatggat ttacaggggc aaacataagt cctcattatg atagtttatt
     3121 ggttaagaca atatcttggg atagaacatt ccaaggagca ataaataaaa ctataagatc
     3181 tataaaagaa cttagagtta gaggtgttaa aactaatgta ggattcctag ttaatgtttt
     3241 aaataatcct atattctcaa atggtaaatg tagtactaag tttatagatg aaaatccaga
     3301 tttatttgaa ataacagaaa gtaaagacag aggaacaaaa ttacttcaat ttataggaga
     3361 tgtaattgta aatgataatg cttgtaaaga aaaaccatta tttgatgcat tacatgaccc
     3421 aagaatggat aaggatggtt caaaatcaga aggaagtaaa atacttttcg ataagttagg
     3481 taaatctgca tatatagaaa agataaaaaa tgataagaaa ttactattaa cagatacaac
     3541 aatgagagat gcacatcaat cattattagc aacaagaata agaacatatg acttattaaa
     3601 agctgctaag ccaacagaaa aatatcaaaa ggatttattc tcacttgaaa tgtggggagg
     3661 agctacttat gatgttgcat atagattttt aaaagaatct ccttggcgta gattacaaaa
     3721 gctaagagaa gaaataccaa gcataatgtt ccaaatgttg cttagagcat caaatggagt
     3781 tggatataaa aactatccag ataatgtaat agaagaattt acaaaagaat ctgctagaca
     3841 aggtatagat gtatttagaa tatttgattc attaaactgg gttgaaaata tgaaaccatc
     3901 tattaataca gctttagaaa ctggaaaaat tgttgaagca actatgtgtt atacaggtga
     3961 tatactagat aagactaaaa ctaaatacaa tctagaatat tatataaaaa tggcacaaga
     4021 attagaaagc ttgggagcag acataatagc aataaaagat atgtcaggtc ttcttaaacc
     4081 atattcagct tacacattag ttaaagagtt aaagaaaaat gtaaaagcac caatacattt
     4141 acacactcat gatacaagtg gaaatggtgt agcaacttgt ttaatggcgt cagaagctgg
     4201 agtagacata attgatgctg cattagaatc tatggcagga cttacaagtc agccatcact
     4261 taatgctata gtagaggcac ttaagaatac agagagagat acaggaatag atttatttgg
     4321 atatgatgag ttaggaaaat attataagga tttaagaaaa gtttataata aatttgaaag
     4381 tgatttaact aattcttgtg cagaaatata taattttgaa ataccaggag gccaatatac
     4441 aaacttaaaa cctcaagctg atagtttagg attagttaat agatttgatg aagttaaaga
     4501 aaaatataaa gaagctaatg aagttgtagg agatataata aaagtaactc catcttcaaa
     4561 agttgtagga gacttagcta tctttatgac taaaaataaa ttagataaag acaatataat
     4621 agaagaaggt aaaaatttat cattcccgga ttctgttgtg gattactgta aaggtatgat
     4681 tggtcaacca gaaggtggaa taccaaaaga tttacaagag gtagttctaa aaggtgaaga
     4741 agctataaca gtaagaccag gttcattatt accagcagaa gattttgatg aaatagctaa
     4801 atatctaaat gaaaagtatg atataaatgc taatataaga aatgttataa gttatgcttt
     4861 atatccaaaa gtatatgaag attatatcaa acatctacaa cattataatg atatatctaa
     4921 acttgaaagt gatgtattct tctatggatt aaataaaaat gaagaatgtg aagtagaaat
     4981 agaagaaggt aaggtattaa caataagatt agttgaaatt ggtgaagtta aggaaaatgg
     5041 atttagaact ataggatttg agttaaatgg aatggttaga gaagtagaaa taaaagataa
     5101 gaatttctct ggaaaaatta ataatgttga aaaagctgat atgaatgacc ctcttcaaat
     5161 tggagcaagc ataccaggaa aagttataaa aataatggtt aaagaagaag atgaagtaaa
     5221 agcaaatcaa ccattaatcg ttatagaggc tatgaagatg gaaactataa tagttgctaa
     5281 gactgatgga gtaattaagt caatcaaagt aaaagaagat gatatggttg aagataaaca
     5341 attattaatg ataatgaagt aatttaatta aaataaatta aaaagcactc tgaaatgttt
     5401 aataaaaaat gttttagagt gtttttattt attttaatta aatatttgtt aaaaaaaaga
     5461 ggacaattac ctccagatgt agaaataaag ctagtaagga aaatgttatc taaaaattct
     5521 aaatatagta aatattttcc ataatatttc ttaggaaata acagcaaaga taaaatacat
     5581 aaatttaaaa ggtgggtgct tatatgaagg tttatgatag taaaatgttg agaaatgtag
     5641 cggtattagg acatagcgga tgtggaaaaa caaatctaat cgaaactata gcttacacag
     5701 caaacactaa taagatacct aaattaacag acaaggtaaa tatgacttat agcatgggat
     5761 taatacctat agagtataat gattataagt ttaacttatt ggatacccca ggatattttg
     5821 actttagcgg agacgttgtg tcatctctta gagcaagtga tgcagcaata atagtaatag
     5881 atgcaacagc gcctattcaa gttggtacag agaaatcatt agaactaact gagagtattc
     5941 ctaagataat gtttataaat aaaatagata atgaaaaggc aagatacaaa gatgcaatag
     6001 caatgttaag agaaaaatac aataataaga tagttcctat gataagtcct atatacaaag
     6061 ataaaaactt tgtaaagtta cataatgttt ttgaaaacat agatgattta gaaggtgaat
     6121 ttaaggaaca agctatgagt gttaaagaag cattaatgga acttatagca gaaactgatg
     6181 accaaatatt agataaatat tttaatggag aagaactaac tacagaagag atacaaaaag
     6241 gtattattat aggtatacaa agaggtgata ttatacctgt aatatgtggt tcaactataa
     6301 ataatatagg cacaaaggaa atattagata caatatcaag ttatttagaa cctatcttta
     6361 cagaggaaag caaaccattt aggggattag tatttaaaac tatggttgac ccatttgttg
     6421 gaaagatgtc atatataaaa attactgagg gtgtactttc taaggataaa gatgttttta
     6481 atataaataa aaatgtaaaa gaaaaaatag ctaatatata tacattgaga aatagtgaat
     6541 tagtagaaat agaaaaagct aaagctggtg atatagtagt tataactaaa gttaattctc
     6601 taaaaacagg agacactata tctgcagata aagatgcaga agctttagaa aagatagatt
     6661 ttcctaaacc acaaatatat tatgcagtaa ctcctaagaa taaaggtgat gaagaaaaag
     6721 tagcatctgt attaaataaa ctagtagaag aggaccctac attacattgg tatagaaata
     6781 cagagacaaa acaagcactt ttaggaggtc aaggagaact tcatataaaa acaattaaaa
     6841 ataaaatgaa agataagttt ggtgttgatg ttgaattgaa tgaccttaaa gttccttata
     6901 gagaaactat aaaaggaaca gctgatgtac aaggaaaaca taaaaagcaa tcaggtggtc
     6961 atggtcaata tggagacgta aaaataagat ttgaaagatg tgaatctgat tttgaattta
     7021 cagaagaaat atttggtggt tctgtaccaa aacaatatat accagcagtt gaaaaaggtt
     7081 taaaagactc tatgcaaaaa ggtatattag caggatatcc tgttacaaac ataaaagcta
     7141 cgctttatga tggttcatac catgatgtag actcttctga aatggccttt aagatggcag
     7201 caagtgcagc ctttaaaaaa ggtatggaag aagcacaccc aatactacta gaacctataa
     7261 tgaaacttaa gataactgtt ccagaagaat atatgggaga tgtcatgggt gatataaata
     7321 agagaagagg aaaaatattt ggaatggaac cagatgacaa agggaaacaa ataatatttg
     7381 cagaagctcc acaagcagag acatttaaat atgcaataga tttaagagca atgacacaag
     7441 gtagaggata ctttgaaatg gaattagaga gatatggaga ggttccatca cagtttgcag
     7501 aaaaaataat tgggttagct actgcaaagt aatgaacaaa ataaatagaa aaataattaa
     7561 tatatattat taaaaatatt tttagggttt taactaggta taggtttatg ttaggtaaac
     7621 ttatacctag tttttataca tttttgatga ataaaaaaat atataatact ttcttaaata
     7681 ttttaataaa aagaaagtat ttttttatta ttatttttac aaatgtttag taataaagat
     7741 gagaaggtat atttaaaata taaagtattt aaagttctga aatctaatca tgaagcaaaa
     7801 aattagcatt tggttatcaa taaatataat aaataaaaac tcataattaa tgaaagaata
     7861 aattcatatc taaatttcta taaaaaattt aaatttatac aatttagggc tttaaaaatt
     7921 taaataatag gtgtaaaata atagtcaaag aaagtcaaaa ataaaaaggt gtgtggtgat
     7981 attaatggca actatgacag atataattga aaaatttatt aaagatttga tggaagaaga
     8041 taatagcata caaattcaaa gaaatgaact agcaaacttg tttagttgtg caccatcaca
     8101 aataaattat gtcttaacaa caagatttac aatagataga ggatattaca ttgaaagtaa
     8161 aaaaggcggc ggaggatatg ttcaaataga aaaaatacga aaaagtaaag atgggcatat
     8221 tagagaacta ctaaatgaaa aaataggaag tcagatatcc tataaaaaag caaaagaatt
     8281 attagaaggt ttaaaagagt cggatttgat taacgaaaga gaattaaaat taatattata
     8341 tgcaatagat gataaatcat tgtgtatgcc aatatatgaa ttaaaagaga aggtaaggtc
     8401 taatatttta aagaatataa taataggatt atttagtata gaggagtgat aatttatgct
     8461 atgtcaaaaa tgcaataaga ataaagcatc tgtatattat aataagatag tcaatggaga
     8521 aaaaacagag atgtatttat gtagtgagtg tgcaaaagag aatacagaaa tgaattttaa
     8581 cttagatatg cctttttcaa tgatggatat attttctaat ttaggttttc aacctaaaaa
     8641 agaacttgag gaaaagctag tttgtcctaa atgcaataca acatatagtg agtttaagaa
     8701 taatgggaga tttggatgta gtgaatgtta taatgcattt agtagtcaag taaatccaat
     8761 gcttcaaaat atacatgggc atatagaaca tacaggaaaa gcacctaaaa aatcttttta
     8821 taagataagt gtagaaaatg agattaaaga actaaaagaa gatttagata gagctataaa
     8881 aaatgaagag tatgagttag cagctcaatt tagagataaa attaaatatt taaaaggttc
     8941 tattgactaa gattggagtg atattatgaa agagaacata gtcatgaaat caagagttag
     9001 gctagctaga aatttaaata actatccatt tccaaataaa ttagataaag aatgtgccat
     9061 ggaaataata gaaaaagtaa aaaatgcgtt tattaattct aacttagaac aaaaagaaga
     9121 atttgatttc tataaaattg aagatttaga ccaatctaaa aaaatgctta tggttgaaga
     9181 acatataata agcccagatt tagcagaaaa tgataaaagt gcagttatag taaaaaaaga
     9241 taaaactata agtataatga taaatgaaga agaccatata aggatacaaa ctatatgtga
     9301 tgatttaaac ttagaatatg catatagtgt agcaaatgaa atagatgatt tgttagaatc
     9361 aagtttagaa tatgccttta acacaaagct aggttatcta acatcttgtc caactaatac
     9421 aggaacagga atgagagctt ctgtaatgat gcatttacca gcattaagtc aactaggcta
     9481 tatggatgaa ctttataaaa tttcatcaca aataggcata gcaattagag ggatatatgg
     9541 agaacgaaca gaagcattgg gaaatatata tcaaatatct aatcaactta cacttggaag
     9601 aacagagtct aatattatag aaaatgtaag tggacttaca aaagatgcaa tttcaaaaga
     9661 aataaaagct agagaaatat tgcaaaaaaa attaggtata aaactagaag ataaaatatt
     9721 tagatcgata gggacacttg aaaattcaag agtaatgagt agcgcagagg caatgagcca
     9781 tctatcaaat ataaaaatgg gaatagaaat gaactatata gataaactag atttaagggc
     9841 tatagaacaa cttatgatag gtatacaacc agcgcatcag tcaataatgt ataagagtga
     9901 tgatgttgag aatagagata taaatagagc aacatatatt agagaaactt tggaaaaatt
     9961 aagaggaggg atgaattgtg aacttcaata gatttacaca aagagctaaa aaggcaattg
    10021 atttagcgtt tgaatctgct aaaagtctag gacacaatat tgtcggaagt gaacatatac
    10081 ttttaggtct tttaagagaa gaagaaggta tagctgctaa agttctaagt aaagttggat
    10141 ttacagaagc ttatttagaa ggcaaaatag ttgatatgga aggtaaggga gaagaaatct
    10201 cagaagatat agtattaagt ccaaggagta aacaaatact tgaattatca ggaatgtttg
    10261 caaataaatt aaaaacaaat tatattggga ctgagcatat attattagca attattcaag
    10321 aaggtgaagg aatagctaat aagattttaa attatgctgg agtaaatgat aggactttag
    10381 cccaattaac aattgatatg atgggtatta gtgataaaaa tcaatacaaa gcagagaata
    10441 gttacacggg caaccagaat caagcagaat ctaaagtact tgataagtat ggaagaaatc
    10501 ttacactata tgcaaaacag aataaaatag accctgtaat aggtagagaa aaagaaatac
    10561 aaagagtaat acaaatatta agtagaagaa ctaaaaacaa tccagtgttg ataggagacc
    10621 caggtgtagg gaaaacagcc atagctgaag gtctggctac aaatatagcc ttaggaaatg
    10681 ttccagaaac actaaaaaac aaaactttat actctttaga gatgggttca ttattggctg
    10741 gagctaagta tagaggagaa tttgaagaaa gaattaaaga agttgtagat gaagttgtta
    10801 aaaatggaaa cataatctta tttatagatg agatgcacac aataataggg gctgggtcta
    10861 ctggagaagg ctctatagat gcttcaaata tattaaagcc agccttagca agaggagaaa
    10921 tacaagttat aggtgcaact acaattgatg aatatagaaa gcatgttgaa aaagattctg
    10981 ctctagaaag aagattccaa ccagttatgg tagatgaacc aactaaagag gactcaataa
    11041 aaatattaga aggattaaga gataaatatg aagctcatca taaagttaaa ataactgatg
    11101 atgctataaa ggcagctgta gaattatcaa ctagatatat atcagacaga tatctgccag
    11161 ataaagctat agacttgatt gatgaagctg catcaaaagt aagattaaaa gaaaatacac
    11221 ctccttcaga aataaaaaaa ttagaactag aaatagaaaa tatagataaa gaaaaagaag
    11281 aagcagtaag atgtcaagac tttgagaaag cagccaaaat aagagatgaa caaggtctac
    11341 ttaaaaaaca attagaagat gttagagaaa gatggaacaa gtcatctaaa cattcagatt
    11401 tagttgatgg agaagttatt gctgaagtag tgggcttatg gacaggaata ccagttaata
    11461 aaattcttga agaagaggct gatagacttt tgagacttga ggaaatactg cacaatagag
    11521 ttataggtca agaacaagca gtaaaatcta tttcaaaggc aatcagaagg tcaagagcag
    11581 gtcttaaaga ccctaataga ccaattggtt catttttatt tttaggtcca acaggagtag
    11641 gtaaaacaga attatctaag gcattagcag aagtgcaatt tggagatgaa aatcaaataa
    11701 ttagaataga tatgtctgaa tatatggaaa aacatgctgt gtcaagaatg ataggttcac
    11761 ctccaggtta tgtaggtcat gatgaaggtg gtcaattaac tgaaaaagta agaagaaatc
    11821 catattcagt tatcttattt gatgagatag aaaaagctca tccagatgta tttaatattc
    11881 tgttacaaat tctagatgat ggtagactaa ctgattcaaa aggaagaact gtagatttta
    11941 agaatactat tgtaataatg acatcaaatg ttggagcatc tacaattggt agacaaaaaa
    12001 ctttaggatt tagtatagct aaaggagacg aagaagaaaa atctcaatat gaaaaaatga
    12061 aagaaaatat aatgggtgaa ttaaaacaaa gattcagacc agagttttta aacagaatag
    12121 atgatataat tgttttccac tcactaaatg aaaatcatat atcaaaaata gtacttttaa
    12181 tggcagcgaa actacaagag agattaaaag aaatggatat aaaattagaa atgagtgatg
    12241 aggctgttaa attaatttct aaatctggat ttgacttaga atatggagca agacctctta
    12301 aaagagcttt acaaaaagag ttggaagatg agctatctga agcaatctta aaaggtaatg
    12361 tgaaaaaggg aagtaatgta gtagcaaaag tcaaagatga aaaaatagtt tttgaaacta
    12421 aataatttaa tagagttgca ttttaaaata ttgcaaatat aagttttata atctaaacaa
    12481 aattttaagg gcaatgaatc tataataatt cattgtcctt aattatttta tttaatcttt
    12541 acatattgtc tttcagatat gctaaaatta tagtatagaa taaaaaatta aggatgataa
    12601 aagtatggca aaaataaaaa caaaatatgt atgtcaatct tgcggatatg aaacagctaa
    12661 atggcttggt aaatgtccag aatgtacaaa atggaataca tttgtagaag aaatagacca
    12721 aaaaagcacc aaaaaagagg tctttataat agataaatct tcatctaaac cagtcagtat
    12781 aaactcaata gagagtaaag aagaagagcg gtttacaaca gatataaatg aattagatag
    12841 agtgcttgga ggaggtatag ttaaaggctc tttagtgctt gttggtggtg accctggtat
    12901 aggtaaatca acacttttaa tccaagtatc tagtaatgtt gccaatttag gaaaaactgt
    12961 tttatatata actggagaag aatcagaatc tcaaataaaa atgagagcta aaagattagg
    13021 tattaactca gaaaatttat atatatttgc tgaaaataat ttaagtataa ttgaatctta
    13081 tttagaaagt gtaaatccag aattaataat tctagactca attcaaactg tcttcagtcc
    13141 agaaatatcc tcggcaccag gaactgtcag ccaaattaaa gaaggtactt caaaattcat
    13201 gaaaatttct aaaaaaatgg gaatttcaac gttcatagtt gggcatgtta caaaagaagg
    13261 ttcactagca ggaccaaaat tattagagca tatggttgat actgttctat attttgaagg
    13321 agaaagatat aatacttata gattagtaag agctgttaaa aatcgatttg gttcaactaa
    13381 tgaacttgga gtgtttgaaa tgagggattt aggattagta gaacttgata atccatctaa
    13441 aattttaatc tctgaaaaac caaaagatgt agcaggttca gtaataatat ctactgtaga
    13501 aggtacgaga ccaatgttac ttgaattgca agcattagtt tctcctacaa gttttggtat
    13561 accaaaaaga acatctacag gagtcgatta caatagagta ggtatgttat tagcagtatt
    13621 agaaaaacgt gttggtttgc aaatacaaaa tcaagatgtc tatataaaca tagttggagg
    13681 aataaagata aatgaaccct caattgattt agggatagcc atttctgtag catctagttt
    13741 tagaaatata ccgatagatg aagatatagc tgttacagga gaagtgggac ttacaggaga
    13801 agttagagca gtaagcttta tagaaaaaag aatagctgag tgtaaaaaac ttggatttaa
    13861 aaagatagta gttcctagaa gtaattatga tgttgtaaaa gagaccaaag gcatagaaat
    13921 atggccagta gataacttaa gacaagctat aaatatagta cttgggagga atcaataaat
    13981 atggagaatt ttctagataa taaaaatatg ctatatgcat taaaaatgat atctcctgga
    14041 actccactta gattaggtct aaacaatgta ctaagagcta agactggtgg attaattgta
    14101 attgcaacaa acgaagatgt aatgaaaata gtagatggag gatttgctat aaatgcagaa
    14161 tattcaccat catatctata tgaattagct aaaatggatg gagctatagt tttaagtggt
    14221 gatgtaaaga aaatattatt tgctaatgca caacttatac ctgactattt tatagaaaca
    14281 tcagagacag gaacaagaca tagaacagca gaaagagtag caaaacaaac tggtgctata
    14341 gtcataggaa tttcacaaag aagaaatgtt ataacagttt atagaggaaa tgagaagtat
    14401 gtagtcgaag atatatctaa gatatttact aaggcaaatc aggctataca aactctggaa
    14461 aaatataaga cagtattgga ccaagctgta acaaatttaa atgccttaga gtttaatgat
    14521 ttggtaacta tttatgatgt tgcattagtc atgcaaaaga tggaaatggt aatgagagtt
    14581 acaagtataa ttgaaaaata tgtgatagaa ttgggtgatg aaggaacttt agtaagtatg
    14641 caattagaag aattaatggg tacaaccaga atagaccaga aattaatatt caaagattat
    14701 aataaagaaa acacagaaat aaaagaactt atgaaaaagg tcaaaaattt aaattcagaa
    14761 gaactaatag aattggttaa tatggcaaaa ctattagggt atagtggttt ttcagaaagt
    14821 atggatatgc ctataaaaac aagaggttat aggattctta gcaaaataca tagactacca
    14881 acagcaataa tagaaaactt agtaaattat tttgaaaact ttcaacaaat tttagatgca
    14941 tctattgaag aattagatga ggttgaagga ataggtgaaa taagagcaac atatataaaa
    15001 aatggactca taaaaatgaa acaattagtc ttattagata gacacatatg atgaatttta
    15061 aatgaatttt actacttttt tcttgaatat aatatacact ttggtctata atagataagt
    15121 ataactaggg ggtgaagcat tgataaggaa agtaacaaga atattgttta ttttacttgg
    15181 atttacaata ggtataacaa cctatttaac attaatgaaa gattttgaaa tattaacatt
    15241 tggaaaagaa acatatggtt atatcgcagc tgtagtagca ggtataataa tagccatttt
    15301 aggatatctt atagaaccat gggttgtaaa taaagtaaaa gaaattgcta aaatagtgga
    15361 taaagaattg tcaaaatatc ctcagactga tatattgtta gggtctatgg gtcttatagt
    15421 aggatttgtt attgcgtatt tattaagtgg attagtaaat agaataccaa tagtaggtgg
    15481 aatactatcc ttactattat atttattcct aggatatctt ggaatgaaag ttgcgcttaa
    15541 aagtaaaaat gatttattta atattggaaa actaggaaga ttagcaaatc ctattaagga
    15601 taaagataag gataaagaaa ataaaaaaga agtaaaagcc attcctccaa aagtattaga
    15661 tacaagtgta ataattgatg gaagaatagc tgatatttgt aagactgggt ttatagaagg
    15721 aaaacttatt attcctgctt ttgttttgga ggaactaaga catattgcag attcttcaga
    15781 tgatttaaaa agagtaagag gtagaagagg tctggatata ttaaatataa ttcagaaaga
    15841 attaaatata gaagttgaaa taagtgagag agactttgat gatatagcag aagttgatag
    15901 taaattatta aagcttgctc aggttttaaa tggaaaagtt gttacaaatg attataactt
    15961 aaataaagtt gctcaattcc aaggtgttga agtattaaat ataaatgagt tagctaatgc
    16021 tataaaacca gttgctatac caggtgaaga tatggttgtt caagtggtta aagaaggaaa
    16081 agaggctcaa caaggtgttg catatctaga tgatggaact atgatagttg ttgatggagg
    16141 aagaaaatat atgaatgaga ctataaaagt actcgttact tctgttcttc aaactcctgc
    16201 aggtagaatg atatttgcaa aacctaaaaa ttaatatgtt caaagaaacc tataatttgg
    16261 gctgtttatt ttaaatagat ggtccaaatt tatttattta taacgataat gtttaatgta
    16321 ttcttaaaga tggggattta aatcagaaag cagggagaat ttatgagaaa aaagattata
    16381 tctttcttag caacatttat cataatatta acatcagcaa gtcagtatag ttttgcagat
    16441 gatatatcaa cacgtggaaa agtaatattt atagatatga atagaactag tatgagtaat
    16501 atgttaagaa taaaatcatt aagagaagaa ctagacaata gagggtatat tggcttgatg
    16561 aatataagag gcgataaagg aagtgatgat agaaggtcat atgctagtat gggtgctggt
    16621 ggtagagcta atgtggctaa tgaagaagat attaattttg aaagctcgag taaggataga
    16681 aatatagttt ttgaatcagc tacaggaaaa agtgcaaaag gtataaataa tttaactata
    16741 aataagtcta taaatgaaaa tttaaacttt ggagagtatg gttctgtatt agggtcatta
    16801 ggacaaagtt tatctgataa tggattaaaa gcatctgtat tgggtaattc agatataatt
    16861 gaaaatggtc aacttataaa aaatagaaac ttatgtctaa ctgcaatgga tgaatatgga
    16921 agaataccta atggaaatgt agatactata aataaaaaag acttatctat gccttatgga
    16981 atatcaactg attatgataa attgattgta gagactaaag aagcttacaa aaataatgat
    17041 gtgatttttg tggagcttgg agacacatat agattagatt tatataaacc aaatttaaat
    17101 gaaaaaacat atgagagtat gaaagatagc atagaaaaaa atatagatat atatttaaaa
    17161 aatatatttt ctatggttga agaaaatgac actgtctaca tagcaagtgc atttccaagt
    17221 gatttagatt ataaaaataa aagaagactt tctccaataa taaaattaaa tggagaaggg
    17281 aaaggtatat tatcatcttc aactactaga agagaaggta ttgtaacaaa tttagatgtt
    17341 ggggttgaaa tattagacaa ctttaatata aaaaatcaaa atatggttgg aagaaaatat
    17401 gaacttatta acagagatga taataaagaa tttttgatgg atgaatatca aaagatagta
    17461 tctatttcaa gcataaggtc tacaatactg aatggttttg taagtatagt tttcttgtca
    17521 tggatagttg ctatgattgc aattttattt agaaagcata tatcaaaaaa ttataaagaa
    17581 acaacatttt ttatattaaa ggaactttta aaaataggaa tagtaatgcc actgtcattt
    17641 atgataacac caataatgaa tttcaaaaca ccattagcaa tatctttagg aataattata
    17701 ataacactta ctatatatct tataagtaaa gttttaataa agaacgattt aaaaaacatg
    17761 ctgtttttta ctggattaac catagtaatt atggttattg atgctgggtt tggctcatat
    17821 ttaatgaaaa gcaatgttat gagttatgat tgtataattg gagctagata ttatggagta
    17881 ggaaatgagt atcaaggtgt ggcaatcggt agtgcaatat ttacatttgc aattttactt
    17941 acatataaaa atataccaaa gtggtctata atagtttttt ctttggttat cttaattaca
    18001 agtgcatctc caattatggg agcaaatgtt ggaagtgcta tttctgaatg tgttgcattt
    18061 ttactattta tactattgat atataatgta aagatagatt tcaaaaaaat cgtattatta
    18121 ggtatagcag tagtatttgt attaggagtt tttgtagcaa tagatatgat tttgggaagt
    18181 aattcacatt taggcatgtt tgtaaaagaa atttacttta atggaccagg agaaataata
    18241 caaacattta gtagaaagat agagatgaat ttaaagctag ctcaaaccag tgcatgggta
    18301 aatatactgt taactggaat aggtgtaatt ttagttctta tgattaatca gattagatac
    18361 tttaagcaac tgatggatga gtatccaatt gtatttaaag gatttatagc atcaatagca
    18421 ggatgtttag taactctttt agtgaatgac tctgggattg tgtcatcagc tacagccttt
    18481 atttatgtaa ttgtacctat gataacatta agtataaatc tcacagcatt aaaagaataa
    18541 gtactgtatg aataatatgt aaatattaat acattaattt aacttcttta tttacaaaat
    18601 aaagaagaaa gtttataaaa aataaattat aataaaaaag agtttatctc aaaattgatt
    18661 attctaatca aattttaaga taaacttttt ttattataaa gataaataac atataacctc
    18721 atattttagg tagatttttt tcttatatgt ttaggagtgc atttaaattt ttccttaaaa
    18781 attctgtaaa ataattttgt attagtaaat ccatgagctt ctaatatact gcataaggta
    18841 tcatttgtgt taattaaatc tttataaata tgagaaatac gcacatcatt taaatactct
    18901 aagtaagttt gtcccatata ctgtttaaat tttctacaaa aatattctgg ttgtaagtgt
    18961 gctaccttag cgattttgtt aatagaaata ggcattttgt aattaatctt tgtatattct
    19021 aaaactggtt caaattttga caaatcaagt actgtttttt tcatattttt attacctaca
    19081 gatattttaa agtttgtata taattcaaat aaaagttcaa ataataagct tgtaaagagc
    19141 agattagctg caggaggaga actaagttta atttctctca tgttttctaa tataagttta
    19201 actttacttg aatttttttt atagttattg tcattggcat taaccttaaa gtcaaaatag
    19261 caatttttaa aatcagggat atatttgttt agaaaagaca atggtacttg caataatata
    19321 gaggtatttt gataaataca tctagttgag tgaataattc cagaattaat gaacatacac
    19381 tcattagctt ttaaattata agtgtaaccc tccatgttaa tttgtaaatc accagaagtt
    19441 atataaatta tttctaatga gttatgccaa tgatttgcta cataactacc actatcatca
    19501 aatagtttga aattaacatc taaattatca ataggttcaa ttatttcatg tgaaaaatta
    19561 tttctgttca taataatatc ctccttataa ttttaaatta taaatagtaa atttataatg
    19621 ttcaatatta aaaagataat atagactaat aaattagata ataaacatac ttaagaaaac
    19681 agataaaatc aaatataata actgtatatt aaaataactt aaaaaaatta ttttattata
    19741 agtgcaaaat atacctattt aaataagata agaggggagg attttatgtg tatataaaag
    19801 gtgtaaattt aggaggttgg ttagttttag aaaaatggat gacctcaagt ttatttgaag
    19861 gcacagaagc tgaagatgaa tactatttac caagacagct ttcaagagag gcatatgaat
    19921 ctagaattaa gactcatcgt agtgaataca tcacagagag agattttgca acgattaaat
    19981 ctatgggatt taattcagtt cgtataccag taccatactt tatttttgga gattgtgaac
    20041 cattcatagg atgtgttaaa gaacttgata aagcatttgc ttgggcagat aagtatgggc
    20101 ttagtatatt gatagactta catacagttc caggaagcca aaatggattt gataatggag
    20161 gaatatcagg tatatgtagc tggtcacaaa atccagaata tgtagcgttt acattaaatg
    20221 tattagaaag acttgcaaaa cgttatggga tgaggcatga attgtatgga atccaaattt
    20281 taaatgagcc tataacagaa agaatgtgga acataatgaa tgttccaaat cggtttaagg
    20341 ctgtagataa ggagatggca agggggagta aacctaattc attagagttt ttacgtgatt
    20401 tttatataaa agcatataag gtaatgcgtc cttatatgag agaggaaaac gttatagtat
    20461 ttcatgatgc atttgaatta aaagcatgga aagactttat gagagaggaa gaatttaaaa
    20521 atgttgttct agatacacac caatatttga tgttagctga agcagatgga tgtgaacaaa
    20581 gtattgatag ctatctcaaa tatattagag aaaattatgc taaagatatt ttacaaatgc
    20641 aaaaatattt tcctgttata tgtggggagt ggagcttatt taattcatat gcttgtggta
    20701 tagatacaaa tggaggtcaa tcacctctaa atgggattga atcaaatata gataaattat
    20761 caaaagacga taaaagagaa ttatatagga agatagcaaa agcacagctt gatgcatgga
    20821 gaaatggaag tggtcactac tattggaatt ataagttatt actagataca gttaatgaag
    20881 aaggttggat aggatgggat agttgggacc taggcaaatg tgtagctcaa gaatggtatc
    20941 ctatagaata ttaaactaaa attggaggaa aagatatgat taaaaaccct atacttccag
    21001 gttttaatcc agatccttgt atttgtagaa agggagatga ttattatctt gtagtttcat
    21061 cttttgaatg gtttccaggt atacctgttt atcactctaa agacttaaaa aattgggagt
    21121 tatatactca tattttgaca gatgaaacaa aaatagattt gaagaagctt ccttcatcta
    21181 aaggaatttg ggcaccttgt ttgacttatt gtgaagaaga agatttattt tacatagttt
    21241 atggaattat gaactctatg aatgcaagat attttgatgt tgataattat ttaattacat
    21301 caaaagatat aaagggagag tggagtgaac cagtatatct acactcatca ggttttgatg
    21361 cgtctatttt tcatgatgat gatggtaaaa agtggattgc atcattagac tgggagacta
    21421 gagaaggata tgaaaaacct ggtgttatat gtttagtgga atactgtaca aaaaagaaag
    21481 aaatagtagg atatccaaag agaatatggt cagggggaac tgatagaggt tgtattgaag
    21541 caccacatat aacaaagcgt ggagattact attatataat gtgtgcagaa ggtggaactg
    21601 gatatggtca tagtgtaact atgggaaggg ctaaaaatat atggggacct tatgaaaaag
    21661 attcaatgaa tccaattgta acttcaattc caggcgattt ttatgaaaga catgaccctg
    21721 accatctaaa gcctaaatac tataatccag aatcaaaact tcaaaaatct ggtcatggaa
    21781 gctatataga aacaacatca ggtgaagtat atttagttca tctaacatct agaccatttg
    21841 taccagagtt aaggtgtact ttaggtagag aaacagctat tcaaaaaatg aaatggacaa
    21901 aggataattg gcttcgaatg gaagatgaaa gtaatttggc aaaagaatac gtatcagaaa
    21961 gtaaactaga agaacattta gtttcatcta taccaagttt tgatgacttt gattcaaatg
    22021 aattaggttt acagtactat gctcctagga tttcaccatt atcatttgca gatgttaagt
    22081 ctagaccagg atatgtaagg attagaggac aagaatcaag aacatcatta aataaagtga
    22141 gcatattggc aagaaaatta acttcagtat atgcaagaat aaccactaag atggagtttt
    22201 acccagaggt acatcagcat agtgcgggtt tgataatgta ttatgataat atgaattata
    22261 taaatttaag aaagtattat agtgaaacat tgggtcaaag tgcactatca ataatacatc
    22321 ttgaaaatgg agagaaaacg gaatttttaa atactaggat accaataaaa gatataccaa
    22381 tatatcttcg tctgtatata caaggtcgta agtcatattt tgaatggagt tatgatgaaa
    22441 aaaactacca gagaatagga aaggtatttg atacaactaa gttttcagat gagtactgta
    22501 aatatggaga atttacaggt acgtttatag gattaacttg tgcagatcgt gtaaagcata
    22561 aacactatgc tgattttgat ttttttgaat atatagtaga tgaatcaaaa gatgtcgatt
    22621 aataaaatca ttactaaaat tttcagagca aaggtaaatc ttatgctctg aaataagaaa
    22681 cattagaagg caaatcttac aaaaaggagt atggtattat gaatacagaa gttaatgtta
    22741 aagcaactac gattaaatat gaaaagattt cattttttga aagaatttct tatggttgtg
    22801 gggatttggg gtgcaatatc atatactcag ctatgtcagc atttttgctg ttttattata
    22861 ctaattatgc agatgttagt gcagcagctg taggtagtat tatgcttgta tctaggatac
    22921 tggatgggtt tagtgactta acaatgggaa ttatagtgga tagaactaaa tccaaatacg
    22981 gtaaggctag gccttggatt ttgagaatgg cgattccttt tgctatagca gcagtattat
    23041 tattttctgt tccatcaaat cttggtatca cttctaaatt aatttatatt tttattacat
    23101 ataacttagt ttctactgtt atttatacag caatcaatgt accctatgca actctaaatt
    23161 cacttataac tcaaaaccag tatgaaagag gagtattaag tatatttaga atgatattag
    23221 ccacttgtgg taccttacta ataaatggac ttacactacc tctagttgaa tactttggaa
    23281 ataatttatc agcatggact aaaacattct ttgtatttgg aatagcatct ataatggtat
    23341 tttttataac atttactgga accaaggaaa gagtaaaagc agttaagcaa gataggaatg
    23401 aagtaatacc atttaaaata ggtattaaat ctttatttag aaataagtac tggatacaaa
    23461 taacattatg tttagtttgt atatttattg tatttgcgat aaatggtggt tcatccgtat
    23521 attatgcaaa gtttattttg ggagatgaaa aattatttgg tcctataaat atggtttcaa
    23581 atatatccca aattatagct atgtttatgg ttgctccttt tataaaaaaa tttggaaaaa
    23641 gaaatgtatt aatagtaggt tcaataatat taatatcgtc aaatgttatg tttataatag
    23701 ttggacaaaa tcatatagga gtaatatttg caagtgctat aaagggaata ggaagtgctg
    23761 gaatagcatc aacaatgttt gctattgttt cagatactat tgaatatgga gaatggaaga
    23821 ctggatatag aactgagggg cttataaata gtgcatctag ttttgggttt aaagtgggaa
    23881 atggattagg ttcagcaata ttaggtgcag tattatcaat tggtggttat gtaggaacat
    23941 cagctactca aagtgattta gctatagtat caataaaagt atgttttata tacctaccta
    24001 tatttataac aatattacaa attatcataa tgtcttttta taagcttgat aaagaatata
    24061 gtactatact taatgaatta aatactaaat aatgaaaaag tttcttataa agtcttaagt
    24121 ttaattaagg tctattgtaa atgttcccta taaaaactat tatatattta tgattattga
    24181 agaattttta gagaagcgaa taataaaaat ataagaagac tttaaatgat gaaatggttg
    24241 gttttttaga aatgatttaa taagttattg ttatttaaag cattataagt ggacaattaa
    24301 attttaattt atggagatga tatttaacaa ttagaagtaa tatataatat gcactaagaa
    24361 gcaatttttc aactatttat gaaggtggtg gtacaagaaa agtatcacct tttttttgtt
    24421 atacaaaaaa acagttgact ttttatatga accccttcta taatgttact gaaattaagt
    24481 aaaatgattg aacaaatgta aagataattg aaaagcttga ttttggagga aagcaaatga
    24541 aaaaaattgg agacttaaga ttaatgatga agtgttgttc actttattac gaagataatt
    24601 tgaatcaaca ggaaatagca aatcaattgg gaatatcaag accaacaata tcaagaatat
    24661 taaaagaagc ttttgaacaa ggaatagtaa aaatacagat agttgatgtt ttgaaaaatg
    24721 attaccaaaa aatagagaga agtttggaga gaaaatataa attaaaagaa gtaatagtag
    24781 tagatgataa acaggatgca ctaacacaaa aacaagaact agcaagagct gtatctgaat
    24841 atttaactag agtagtaaag gaaaatgata taataggtgt ttcaatagga acaacactaa
    24901 aagaaatacc aagatatgtg gaaaaaagta actgtaaaaa tgttacattt atacctctat
    24961 taggagggat tggagacaat gaaatagata ttcatgcaaa tcaaatagca gtaagtttag
    25021 cgagggcttt tggaggtgat tttaaattat tacatgctcc agctgttatg tctgacttgt
    25081 ctacaaaaga gaaactttgc aaagacgaaa aaattaaaga agtactagat ttaatagata
    25141 aaacaactat agcaattgtg ggtataggaa atcctatgag tttaaactca acaataatgg
    25201 cttctggata catgaatgaa gcagatatag aagatttaaa aaaatacaac tcaataggtg
    25261 caatttgttt acaagccttt gataaagaag gaaaaacttc tatacttgaa tttaatcaaa
    25321 gagtattagg agtgaaactg gaagatttaa aaaagataaa aagaacaata ggtgtcgctt
    25381 caggagatga aaaaatagag gcaataaagg catcattaaa agcgaaattt ataaatagtt
    25441 tagcaattaa tcacagttta gcacttaaat tattagaaga ttgtgactaa tagaaagata
    25501 gataaaagtt aacaggaggg aatgtatatg tcaatatcaa aagaaacatt gcttgaaatg
    25561 tataagagaa tgaatcaggc tagaaaattt gaagagaaag ttagttggtt ttttgcaaga
    25621 ggtatggttc atggaacaac tcacttatct gtaggtcaag aagcgtcaag tgtggcggct
    25681 gtaatggctt tagaaaaagg agatttagta tcattaactc atagaggaca tagtcaattt
    25741 ataggaatgg gaatagacct aaataaaatg atggctgaac ttatgggtaa agaaactgga
    25801 ttttgtaagg gaaaaggagg ctcaatgcat atagcagata ttgagtcagg taacttagga
    25861 gcaaatggag ttgtaggtgg aggtcttaca atagctccag gagctgcact tacacaacag
    25921 tataaaaaaa caggcaaaat agttttatgt agttttggtg atggagcatc aaatgaaggt
    25981 acttttcatg aaggaataaa tttatcttca atatggaaat tgccaataat attttactgt
    26041 gagaataatt tatacggtat gtcaacaagc ataaaacgtc atatgaatat agaaagtatt
    26101 gccactagag cagcttctta tggaatagaa ggaatatcaa tagatggata taacccaata
    26161 gaagtatacg aaacagtaca aaaagctgct gagaagtgta gaagaggaga aggacctgta
    26221 ttaattgaaa gtagaactta tagatggtta ggtcattcaa aatcagatgc aaatgtatat
    26281 agaacaaaag aagaaataga atcatggaaa gcaaaagacc ctattgaatt tttaaaaaat
    26341 tatcttatag aaaataatct atctaatgaa gatgaattag ataagattca agaatttgca
    26401 aagcaatcaa tagaagatgc agtagagttt gctcaaaata gcccaaatcc aaaaatagag
    26461 tctttattag aggatgtata tgcagactaa gaatggtaaa ggaggaaaat aaaaatgagt
    26521 acaagggaat taacatatgc acaagcaata aaggaagcaa tgtcagaaga aatgagaaga
    26581 gatgaaaatg taatatttat gggagaagat ataggaatat atggaggagc atttggtgta
    26641 tcagttggta tgatagatga atttggacca gaaagagtaa gggatactcc tatatcagaa
    26701 gcagctatag caggagcggc agcaggtgca gcagcaactg gcttaagacc tattatggaa
    26761 gtaatgttta tggactttgt aacaatttct atggatgcaa ttgtaaatca agcggcaaaa
    26821 atgagatata tgtttggagg aaaagcacaa gttccaatgg ttgttagatg tccaggaggt
    26881 tctggaactg gttcagcaga gcaacattca caaagtttag aagcttggtt ttgtcatgta
    26941 ccaggagtaa aagtagttgc accttctaca ccagcagatg ctaagggatt gttgaaagca
    27001 gctataagag ataataatcc agtcatattt gttgagaata aattgttata cagaaaaaaa
    27061 ggttttgtgc cagaagatga ttatgtaatt gaaattggaa aggcagatat taaaagagag
    27121 ggtactgatg ttacagtaat tacttatgga agaatgttac aaagtgttga agaagcagca
    27181 gagactctat caaaagaaaa tataaatgtt gaaattatag atttaagaac tttatatcca
    27241 ttagataaag agactatagt aaagagtgta tgtaaaactg ggagagtttt aatttgccat
    27301 gaagcagcta agactggtgg gctaggtgga gaaatatcag ccctgataac agaaagtgaa
    27361 tcatttgatt atttagatgc tccagtaaaa agaatttgtg gaaaagatgt gccaatacca
    27421 tataatccag agttagaaaa agcagttgtc ccaagagttg atgaaataga agaagcaata
    27481 aaatctttaa ttgttagata aggagggata gatatggtgg caaataaaat aaaagccaca
    27541 ccagcagcta gaagtcaggc aagaaaagac aatataaaat tagataggct gattggaagt
    27601 ggagaaaatg gaaggattca tttagtagat gtgttaaatt atttaaaaga taataaagca
    27661 aatactactc ctttagctag aagaatagca gaagatttaa atatagattt agaaactatt
    27721 gtaggtactg gatataatgg aaagataaga aagtgtgatg tggaaaaatt aactgcaaaa
    27781 gaaactattg tatcaactaa tacatctaaa tctagtgaga aaaaagagct gaaaattgaa
    27841 aatgaaaatt caagcatgtt taacacagta gaaggaatat ttgaaaagcc aaatccaatg
    27901 agagccactg tagcaaaacg aatgtcagaa agttatttta gtgctcctgt atttacattc
    27961 aatatagaag tagatgctac agaattgaag gtacttagag caaagttaat cgacacagta
    28021 aaagaatcaa ctggagtaaa attaactatg acagatttaa tagtaatggc agtatctaaa
    28081 atacttccaa atcatcaagc cttaaattca gcatggacag atgagggtat atttagatat
    28141 aaagatgtaa atatagctat tgcagttggt ttagatgaag gcttatatgt tccggtagta
    28201 aaaaatgcaa acaaaaagtc tttaaaagaa atagcaaaag aaagcaaaga acttgcggaa
    28261 aaagtaaaga cagggaaact tatgccagca gaccaagaag gtaatacatt tacaataagt
    28321 aatgttggaa tgtatggtat aactacattt acacctataa taaatatgcc ttcaagtgca
    28381 atcttagggg taggtgctac acaagataag tttgttccag taaatggaga ggctaaaata
    28441 aaacctataa tgaacttatc attaacatca gaccatagag taatagatgg aacagtagct
    28501 gcaaaattct taaaagattt aaaagaattg ttagaaaatc ctttatcaat gcttgtatag
    28561 gttttgatat tttgttactg atataaatag tttgaataga ttatattatt ataattgttt
    28621 cagtaaattt aaagctttta tatattaaga attattttta acagtaacta tatgtgaata
    28681 tgatttgctt aattaaaata taagaaaata aataggagga gagcctatgt cagtagaagt
    28741 aataatgcca aaagcaggag tagccatgga agaaggtact atagtatctt ggctaaagca
    28801 agaaggcgaa gaagtaaaga ttggagaacc tatactagaa ataacaacag ataaagttaa
    28861 tatggagata gagtcagaag gagaaggtac acttgctgta atcatacata aagaggaagg
    28921 tgaagtatta ccagtattta cagtaatagg tgtaatagca gaaaaagggg aaaatcaaga
    28981 ggaagtaaaa gctaaatatt tatctggaaa tgtttcaaaa gaagatacag ttaaagagaa
    29041 tcaaaatata gaagttaaag aagaaaaaat aaacaaaaaa gagtgtaatc atgattatga
    29101 tgtagtggta ataggaggag gacctggggg atatttatca gcattaaaag cagctctttt
    29161 aggtggaaga gttgctttag ttgaagaaaa tatactagga ggtacttgct taaatagagg
    29221 ttgtatccct acaaagactt atataaaaac agcagaaata ttagaagaaa ttgaccaatt
    29281 atcaaagaga ggtgtaaaag taactgtaga taaagaacag gacattaaga aggccataaa
    29341 atataaaaat agagtagtta aaaaacttac agctggtgtt ggcggattat taaaaagtag
    29401 agatgtagat gtgtttaatc ttaaagctag tgttaaagaa gagcataaag taattttatc
    29461 agatggaaaa gttttagata cagaaaatat aataattgct acaggttcca aagtaaggat
    29521 attacctata aaaggaatag aatcaaattt aataataact agtacagaag cattagattt
    29581 ggaaacagtg ccagaagaat tagtaataat tggtggtgga gtgattggtt gtgaatttgc
    29641 agagatattc aattcaagag gttctaaagt aactatagta gaaatggaag atagagtaat
    29701 cccaagaatg gataaagagt taagcgaatc tttaaaatat tctcttagta aaaaaggtat
    29761 aaatgtatta actaaaaaga aagtttctga atttaaagaa gaaggaaata acatattagt
    29821 gtgcattgaa ggtgaagagc caataaaagc agatttatgt ctatatgcta taggaagaga
    29881 agctaatctt tctggcatag aggacttaga tattaaaata gataaaggtt caatagttgt
    29941 aaatagtaaa atggaaacaa gtatgccaag catatatgca gtaggtgatg ttactggagg
    30001 tgtaatgtta gctcatgctg cttttaaaat gggagaggta gcagcttcaa atgctctagg
    30061 tgtgaataaa gaagttgact taggtgcatt acctagttgt gtttatacaa ttccagaagt
    30121 agcagcagtt ggtattacag aagaggatgc tagaaaaaaa tataatgtaa aagttggtaa
    30181 gtttaatttt gctggaaatg gaagagcttt agcttctggt caagaacaag gatatgtgaa
    30241 ggtagtagca gatgctaaat atggagaaat tttaggtata catatgtttg gatgtggtgt
    30301 agcagaactt ataaatcatg cagcatcatt taaagcgtta gagataccta ctgatgaagc
    30361 aagtgagttg atatttggtc atccatgtac ttcagaagct ttaatggaag ctttggcaga
    30421 tgtaaatgga gaatgtttac acttacctaa aaaataaatc taaacataaa ttattaattt
    30481 aagtctatta ttaatattaa tttatatcat aaaattttaa aacacttact tagccatacc
    30541 ccttattatg caaataattt atttgcataa taaggggtat tttttatgga aaaatataga
    30601 atatagaatc cttgtagttt ttgttgtaca aaaaagatag taatataaaa taacattatc
    30661 attttagaat gatttcaatt gtaattagga aaagtgattt ttttgttttt aaaaaaagta
    30721 ttatttgtaa aatataaaaa aaaatttctt aatatttgaa taaaagaata gcttaattta
    30781 aatgttatta ctttataaaa aaataataag tacaaaaaaa acaatatatg gcatataagt
    30841 tgctatataa ataataagga gggaacaatt ataaatgagg caaagaataa aaaacttaat
    30901 tgaaaatgaa gataagaaaa atcctttaac agatgaagtg atttcaagta ggttaaatat
    30961 tcgaagagaa gatgttacct ttttgagaaa tgagttaaaa atagaagatt caagacaaag
    31021 aagaaaagtt gttttaatta aagctataaa agatatttta aaagaagaaa aaactataaa
    31081 taaaaatgat ataacaagaa gattaaacag cattgggttt acagtatcaa gatttacagt
    31141 catacaatac ttaaaagaga ttcaagattc tggtgaccta aaacttgata aagagagtga
    31201 tgataaaaaa gaagaaataa tcaataatag agaaaataaa gatatagcat ttgataaact
    31261 cataggtagt agtggtagct taagtacaat tataaaacta gccaaagcag caattcttta
    31321 tccacctcat ggtcttcata ctattattat ggggccaact ggtgttggaa agagtgagtt
    31381 agcagagtgt atgtataaat ttgcagtaga atcatccaga ttccctaaga atgcaccatt
    31441 tatagttttt aatgcagcag attattcaga gaatccacag cttttgctat cacagttatt
    31501 tggacacgtt aaaggtgctt ttacaggagc tgatgagaac aaagaaggat tagtatcaaa
    31561 ggcagatgga ggaatattgt ttatagatga aatacataga ttacctcatg aaggtcaaga
    31621 aatactattt caactgattg ataaaggtat ggtcagaaag ttaggagaaa caagactaac
    31681 acataaaata gatgtaatga ttatttcagc aacttctgag ccaatagatt ctcatttact
    31741 taatacattt aaaagacgta tacctgtaac aattgaaata ccagaattag ttgcaagacc
    31801 attaaatgag aggtttgaca ttataaataa tttcttctta atagaagctc aaagaatggg
    31861 agcaaacata catataaagt cagatgtatt aaaagctctt atgctttacg attgtatagg
    31921 taatgtaggt caacttagaa gtgatattca agtagcttgt gctaggggac tattaaacca
    31981 acttacaaac aaactaaaag aagtaaatat aagcatatca gatgtacctg gatatgtaaa
    32041 acaaggtctt ataaaaatca gaaattgtag aggcaaaata gaaaattatg tagatgggga
    32101 tttaattata gattcaaatc ttcaaggtga gatagaaaag aaaaatgaag atatatatac
    32161 ttttccagat gaaatataca aacttactga aagaagacat attgatttat tggaacaagg
    32221 tttagatttt gatgttataa atagaatcat tggaggagaa ttagaaagtt gcattcaaaa
    32281 atacataaaa caagttaaaa aagtttctat tagtaacaat cttccagata taagtccaat
    32341 tgttggaaaa tatatcgtag agctaactta tgaagtaatc aaaatagcat cacaatacct
    32401 aagttcacta gaaccaaatc tacatttatg tttagcagtt cacctaaaag cagcttttga
    32461 gagattaaaa gaaggaaaag taatatcaaa tgctcatttg aaagaaataa taggaaatta
    32521 tccagtagaa tattctgtag cagaaaaggt agctcaatat ttaaaggaaa gatataacat
    32581 aaacttacca aaagaagaaa taggtataat agcaatatat tttaaaatga gtagtagaag
    32641 agatatgtct gaacaaaaaa agataggcgt acttcttatg gctcacggaa gagtagcatc
    32701 ttctgtatgt gaggttacaa ataaactatt aggaattaat cacgcaaggt atttagatat
    32761 gccatttgag aagaagccgg aagaaatgat tgaagaagct actacaataa taaaagatat
    32821 tgatgaaggt agaggtgtga ttatattagt agatatgggt tcactttcat attttggaga
    32881 tattatttct ggaaagactg gaattaatat aaggacaata tctagatttg atacattgct
    32941 ttctatagag gttatacgaa gagcagtttt accagattta acaatagacg agatagttga
    33001 tgaagttaaa ggtatagagc cacaagtaaa agaaaaggtt agtaaatgta acaatacaaa
    33061 agtagatttt agaaaaccta taattttaac attatgtatt actggattag ggggagcttg
    33121 taagataaaa aaaatcttag aggaaaatat taaaagtata actgaaaagg ttgatataga
    33181 acctataggt atgattgaat atccaagtct tgatttagag ataaaacaaa taagcaatga
    33241 taaaaatgta atcgccatag ttggaacaat tgaccctcag atagcaaata tccctttttt
    33301 tagtctagta gacataaatg ataaagcaaa gttaaatcaa ataaataacc taattgatac
    33361 aaatcaaatt ggtgaaacaa ataaaaaata tgaattatca gatttaatac ataaagaaga
    33421 tatatttgtg gatttacagg ttaaatcaaa agaagaagtt ctaaaatata tgagtaaaaa
    33481 acttagagag aaaggttatg tgacaaaaaa tttctataaa aaagtagtgg aaagagataa
    33541 cttatttcca actgaattat cagatgaaat agcaatacct cacacagatg gaatagatgt
    33601 aattaggcca gctataagta tagccatctt aaagaagcct attgtgtgga gtaaaagtaa
    33661 agtaaaaata atacttttat tagctgtaga ccaaaagtgt tttaatccat taagcactct
    33721 actctcattt atagaaacag atgaatttaa gaagataaaa aacattaaag atagagattt
    33781 tattagggag atgatattag atggagtcaa tgaaaataat tagtagcaat ttaattttta
    33841 aaaatattga agtgtctaac aatgaagatg cattaaagtt tttgggacaa cgtttgtttg
    33901 acgaacagta tgtaaaagaa agctacatac aagctgttgt agcaagagaa aaaaagtatg
    33961 ctacagggtt accaacagaa atatacggag tggcaatacc acatacagat atagtacatg
    34021 taaatgagcc aggaatagca attggaatac tgaataaacc tgtaaaattt ataatgatgg
    34081 gtacagacga tactgaaatt gatgtaaaag ttgtatttat gttagcagtg aaagagccac
    34141 aagaacaatt gcaactatta gaaaaactta tgactatatt tcaagataag aatatactaa
    34201 ataatattgt tgacttaagt gaagaaagtg tgagtgattt attaaactct aaattgaaaa
    34261 attaaacatg taaagtatat taaaaatgta aaattaaaca tgcaaagtat gttaaaaaat
    34321 ataaaaagat tattaaagat ttctaactat agatatcttt aaaataaaat aatttcaatg
    34381 ggggtgacta atgtggctaa aaagatatta gttgcatgtg gaacaggagt atgtacttca
    34441 acaattgcta taaacaaatt aaagaaagca cttcaagaca ttggtaaatt ggatatggtt
    34501 aatattacac aatgtaaagt agtagaagtt gcatcaaaag cacctgatta tgatttaata
    34561 atttgtacta cacaagtatc tagtagtata aaaacaccag tgataaatgg attacctttt
    34621 ttaactggtg tcggaatgga taaattaatc aatgatgtat tagaagagtt agaattataa
    34681 ttaaaaaaat taaggaacat ggtctctaaa atcataagtt ttagagactt aaaattaaat
    34741 aatggagggt ctttatatgg agatattaaa tttcattgtt ggtctaggtg cacaagtaat
    34801 gatgccaata ataatatgta tttttggttt gatattaggt acaaaactag gaaaatcttt
    34861 aagagctggt ttaactgtgg gagttgggtt tataggtctt aatactatta tagctttgct
    34921 aacagataat ttaggtccag ctacacaaca aatggttaag aatttagggc taagtttatc
    34981 aataatagat gtaggatggc cagcagcatc agctatagct tttgcatcaa cagtaggggc
    35041 tttaattatt ccaataggtc ttgtggtaaa tatagttatg ctaattacta atactactca
    35101 aactgttgat gtagacatat gggattactg gcattttgct tttacaggag ctttagtggc
    35161 aggggctact caaagtgtaa tgtggggagt atttgcagca gtagcaaata tggtaattgt
    35221 attggtaatg gcagatttaa ctgctccagg tatagaggaa tatttaggta tgccaggtat
    35281 atctcttcct catggattta cacaggcctt tgttccaatt gctatagtag taaataaatt
    35341 attagatcta ataccaggta taaataagat tgagataaat gctgatacat tgcagaaaaa
    35401 atttggatta tttggagaac ctttaataat ggggtctgta ataggtgtaa taattggtat
    35461 tgctgcgaaa tatgatataa aaggaatatt acaattaggt gtaactatgg gagctgtatt
    35521 aatattaatt ccaaagatgg cagcattatt aatggaagga ttattaccag tatcagaagc
    35581 tgcacaggaa tttattgaga agagatttaa aaacagaggc aagatttata taggcttaga
    35641 ttcagcagta ggaataggtc atcctgttac attatcagtt gcactagtgt tggttccttt
    35701 gactatactt atagcagcta tattgccagg aaataaagtt ttaccatttg cagatttagc
    35761 agtaattcca tttgcacttg tattaatcgt accaattaca aagggaaatg tgtttagaac
    35821 attaataata ggaataatta taattacttc tggattatta atcgcaacta acttagctcc
    35881 attatttacc caaatggctt taaatgctag ttttaaaatg cctgaaggag caactatgat
    35941 ttcaagtatt tgtgatggtg cgaatccatt aagttgggta tttgtaaaag ttatgaacta
    36001 taaagttatt ggaggagtag tttttggagt tatagcttta ggaatggcta tttacaatag
    36061 gaatagaata ataaatgaaa ataaaaagct atctatagaa gaataaggtt aaaacaattt
    36121 tattaaggag tgtataggtt atgaaaaaat ataaatatga ttctaaaaga tacttactca
    36181 gtgttacaat accaggattt tttatgattt taatattgat ttatgcactg tttaataact
    36241 atacaaattt taatttgaat atatatagtt tgttaataat tgtctgtact tatagtatat
    36301 tcaatacttt tatatcatca agtaatccta aaaaaattat tattgatagc aaatgtatac
    36361 attttacctc atttatgact acacataagt atgaacttaa agatatagaa aagataagaa
    36421 taagagaatt ttataataaa aaaatttatc taaaaattaa tgatggaaac ctttttaaag
    36481 gtaggtattg gataagaaca aatatgttta ataatagtat agcattatat agctatttca
    36541 ttgagttgga agagtgctta tatccagatt cactaaaatt tagaaataaa aaatttgaga
    36601 ataagaacat ataaaaataa aaaattgaat tgaagcctag cttaatcttt tttgataata
    36661 tataagtttg aactttatac taaattttta gaattttcaa tcaagataat aacataatag
    36721 ctaagcaatt agaacacaat taagatgtat aaggcatgta tatgatgaat gggaggattt
    36781 aattaatgtt attagagaaa ttaagaaaag aagtattaca agcatcttta gaccttttaa
    36841 actataattt agtgacatta acaggaggaa atgtaagtgg gagagatgaa caaactggct
    36901 atatagcaat caccccaagt ggaatggatt atagaaatct tacaccaagt gatattgtta
    36961 tagtagatgt agatggaaat attatagatg gaaaatggaa agcatcagtg gatttatcag
    37021 accacttgta tatatataag catagagaag acataaatag tataattcat acacattcta
    37081 catactcaag ttgttttgct attttaaatg aacctataga atgcgcaagt acaactcttg
    37141 caaatgaagt agggggttca gtaccagttg caaaattttc accaccaaca tctaaaaaga
    37201 tgggaaaatg tgttattgaa gcaattggtg acaaaagagc atgtctttta gcaaatcatg
    37261 gcgtaattgc agttggtcca tctgtaggac atgctcttac agccgctgta atgttagaag
    37321 attcagcaaa agtatattac ttagcaaaat caattggcac accagtgcta ctgcctgatg
    37381 aagaaattca aagagcgaga gatgtatttt ttaatgtcta tggtcaggat aaataaatta
    37441 gataaaattt taaaagtaat atgaaaatat agatattcaa tgatattaag gagatttata
    37501 atgatataat aagtttaata gttaagaagt aaaatatgaa atgtaatagt atataaattc
    37561 atttaagact aaaaagaata agataaaata aaaaggagtc tgtatttcta tgtattactt
    37621 attaaacaaa agcacaaatc cttactttaa tttggcatta gaagaatatc tatttttaaa
    37681 tgataaatat aatgatgata ttataatcat atggagaaat gaagaatcca tttttatagg
    37741 taaaaatcaa aatccatatc aggaagtata tcatgatgtt attgaaaagg gagaaattcc
    37801 tattttaaga agaatatctg gaggaggaac tgtttatcat gacttaggta atatcaatat
    37861 gtcattcata caaaaagata ggcaacttca tgagatagat tttttggagc atactaagtt
    37921 tatgcaaaag atgctttcaa ccttaggact agatgtatct ataactgaaa gaaaagattt
    37981 atttttaaat gggaaaaaaa tttcaggttc tgcacagtct ataaaaagaa aaaattctct
    38041 ttatcatgga actcttcttt acgattcaga tttaaataaa ttaactaaat atttaaattc
    38101 aaataaagca actgaatcaa atgcaactaa atctgtttca agtaaagtta caaatattaa
    38161 gtttttactc gaaaaagata taaattactt tttagattat tgtgttgaat atttaaaatc
    38221 taatataaag aatataaaag aacttgaatt ggatgaagaa gatattaaaa gtgtatacaa
    38281 tataggagaa gataaatata aaaagataga gtggacatat ggtaaaacgc cgaaatttca
    38341 agtagtgcta ccatttaata atgaaagtaa aataaatata catgttaaca gatggaaagt
    38401 atcaaaattt tttatttctt atgatgataa tgtaattaat atggacaagt tgttagattt
    38461 gaatttcttt aaagaagaaa taaaagagag tattttagaa aattatccac attacataga
    38521 tttaatcaat ttaatatttt aatataaaac ctctaatttg ataaaattgt cttatatcaa
    38581 actagaggtt tttaatattc ttgcatatta acaaccatta caaaattgta aaattaaaaa
    38641 caacactatt gaaaaatcaa tatatatttg tttaaatata tgtgtttttc attagcaaat
    38701 agacattaac tatttataca actgaaaatt taatatataa ttaatcagat ggttaaaata
    38761 aaaatattaa aagttttaat aaatgatggt taaaaataaa atacgaggtg ataccatgta
    38821 tagtgttata attgttgcag cgggaagcgg aaggagaatg aacttagata taaacaaaca
    38881 gttcataaaa ttaagagaaa aagaaattat tgctcataca attcaagttt tttatgaaaa
    38941 tataaatata gatgaaatag tggtatgtat aaaaaaagaa gaagaagatt tttttaaaga
    39001 aaatataata aataagtata actttaaaaa tataaaaata gcatatggcg gaaaagaaag
    39061 acaggactca atatataatg gtttaaaaaa attagataaa aattgtgata tagtacttat
    39121 ccatgatgga gcaagaccat ttgtcgacca tagaataatc aacgaatcta taaaggtagc
    39181 taaagaaaaa aaggctgtgg ttgtaggtgt tccagtaagt gatactataa agatagtgtc
    39241 agatggaaca gtaaaagaaa ctccagaaag aaatttgctg tgggcagcac aaacccctca
    39301 aacctttgaa tataatctca taatagatgc ttatgaacaa gcatataaga acaattatta
    39361 tggtactgat gattcaatgt tggtagaaaa tataggtcaa agtgtcacta tggtaatggg
    39421 ttcttatgaa aacataaaaa taactagtcc agaagattta aatatagcag aacaaatatt
    39481 aaatatggaa aaaagagatg aagttaacag tagaaggaga attatatgag aataggatta
    39541 ggatatgatg tacacaaatt aacagaggat agaaaactta taatcggtgg tgtagagata
    39601 ccacacgata aaggtttgtt gggtcattct gatgctgatg tattgataca tgcaataatg
    39661 gattctattt taggtgctct agcattagga gatataggga agcatttccc agatacagat
    39721 gaagaatata agggagcaga tagtatgaaa ttattagaac atgtgtataa tctaataaca
    39781 agtaaaggat ataaaattgg taatatagac agtactataa ttgctcaaag ccctaagatg
    39841 gctccatata ttgagagtat gagaagtaat atatcaaaag ttttaaacac agacattgat
    39901 aatataaaca taaaggcaac aacagaagaa ggtcttggtt ttacaggagc aaaacaggga
    39961 atagcttcac aaagtatttg tttattattg ttgacaagtc agaataatta atatattatt
    40021 agtaataagt ttaatggata tcaagtaata ggcctatata gaatatctat aatgtatgaa
    40081 tttaagtgaa attaatataa atataaaatt agctatgata aagaaatagt aaaatgatga
    40141 aatcttcaca gagagaaaac ggtggtgaga gttttcaaga ggaagtcttt gaacctgtct
    40201 tttagcttaa aggtataaaa atctttacgg taggaaccgt tatattccca aaagagagct
    40261 attcatatag ctaactagag tggtaccgcg gaaattaaac ctttcgtctc tatacttgta
    40321 tagagatgag aggtttttta tattttcagg aagttaatat tcaggaggaa tctattaatg
    40381 aaaatgtcaa aaatgtttat gccgacatta aaagaaatcc cagcagatgc tgagattaca
    40441 agccatcaat taatggttag gtcaggcatg ataaaaaaaa tgacttcagg tgtttataac
    40501 caattaccaa tggggttaag agtgttcaaa aaaattgaac aaataattag agaagaatta
    40561 aataaaaaag attgtcaaga aatactttgt gcagccttac ttccttctga attatggaaa
    40621 gagtctggaa ggtggactgc tatgggtgaa gaaatgttta gactaaaaga cagaacagaa
    40681 agagagtact gtcttgggcc aacacatgaa gaagcattca ctgatataat aagacaagaa
    40741 attacttcat ataaacaatt gccactgaac ttgtaccaaa tacaagtaaa atatagagac
    40801 gagagaaggc caagatttgg tgttatgaga actaagactt ttacaatgaa ggatgcctat
    40861 agttttgatg tagatgataa aggtttagat aagtcatatc aagatatgtt tgatgcatat
    40921 gtaagtatat ttaatagatg tggattggag aatagccctg tacaggcaga ttcaggagct
    40981 ataggaggtt ctacatcagc cgaatttatg gttaagtcag aagttggtga agatgaagtt
    41041 gtattttgta gtggatgtga ttatgcagcc aatgtagaaa gagcagaatc ttgtaatcta
    41101 gcatcacaaa aagaagaaat gaaagaatta gaagaggttc atacaccagg agcagctaca
    41161 ataaaagagt tagaagaatt tttaaaaact tctccagata agtttgctaa aactttagta
    41221 tatgaggcag atggaaaaac agttgtagtc gttgtaagag gagatagaga agttaatgaa
    41281 atcaaagtat ctaatgccat aggttctgtt atagaatttg ctcttgctac agatgatgta
    41341 gtgagaaaag taactaatgc agaagtagga tttgcaggtc caataggtat aaatgctgac
    41401 tatgtattta tagataaaga aattgttgaa caaagaaaca tagttgttgg ggctaataaa
    41461 actgagtacc atataaaaaa tgctaactat ggaagagatt ttgaaggaat agttggagac
    41521 tttagaaatg ttcaagaagg cgacaagtgt atagtatgtg gtaagccact tgaaatagct
    41581 agaggtgttg aagtaggaca tatatttaaa ctaggaacta agtactcaga atctatgaat
    41641 gcaaacttca tagacaaaga tggaaaatca aagcctatag taatgggatg ttatggaata
    41701 ggtgttgaaa gaacagcagc agctataatt gaacaacata atgatgagaa aggtataata
    41761 tggcctttgt cagtagctcc ataccatgtt gtgatagttc cagcaaatat gaaaaatgaa
    41821 gaacaaatat caatagctga aaatatatat aatgatttgc aagctatggg tgtagaagtt
    41881 ttattagatg acagagatga aagaataggt gtcaaattca atgattcaga attaatagga
    41941 atacctatga ggattacagt aggtaaaaat attaatgaag gtaaagtaga atttaaactt
    42001 agacataaag aagataaaga gataattgac atagaagaaa taaatgaaaa agtaaaggca
    42061 gagtttatca ggaataatgt taggttagga taataactta aataagatat tgtatagata
    42121 atcaaagttg tactttatag acaccatttt atctatatat aaggatataa aatggtgttt
    42181 ttttataaaa caaataaatg aggaggtaat taaaatggca aaaaatgaaa aacaatttgt
    42241 tgaagaaata acaaagatgg aagatgattt tccacaatgg tacacagatg taataactaa
    42301 gactgactta gttgattatg caccagtaaa aggttttatg gttgtaaagc catatggata
    42361 tgcattatgg gaaaagatgc aagaatttat ggataaaaaa tttaaagaaa ctggacataa
    42421 aaactgttat tttccattat taataccaga gagtttatta aacaaagaag cagaacacgt
    42481 tgaaggtttt gctccagaag ttgcatgggt aacacatgga ggaaataaaa aattagaaga
    42541 aagattatgt gtaagaccta cttcagagac tataatatgt acaatgtatg cgaaatggtt
    42601 aaaatcatat agagaattac catatcttta taatcaatgg tgctcagttg ttagatggga
    42661 aaaatcaact agacctttct taagaacatc tgagttttta tggcaagaag gtcatacatt
    42721 acatgaaaca gcagaagaag cacaagaaga aacaatacaa caattagaag tatataaagc
    42781 gctatgtgaa gaattacttg caatgccagt tgtagctggt caaaaaagtg aaagtgagaa
    42841 atttgcaggt ggtgaaagaa catatactat agaagctatg atgcatgatg gtaaagcatt
    42901 acaatcaggt acaagtcact tcctaggtca acactttact aaggcttttg atattacttt
    42961 tgcagatagg gaaggaaatt tagctaatcc ataccataca tcttggggag catctacaag
    43021 acttataggt ggacttataa tgactcatag tgataacaga ggtttagtat taccaccaag
    43081 agtagctcca attcaagttg ttatagttcc tatagctgct aaaaaaggaa atgtaatgga
    43141 gactgttgat aaaatatatg ctgatttaaa agctaaggga gtagctgtag aagttgatga
    43201 tagagataac tatacaactg gatggaaatt taatgagtgg gaaatgaaag gtgttccagt
    43261 aagagttgaa attggaccaa aagatataga aaataatcaa gctatggtat ttagaagaga
    43321 tactttagaa aaggattcta tgcctttaga aggattagct gatgctatat gtgatttatt
    43381 tgatgttata cacaatgata tgtttgaaaa agcaagaaaa catagagaag acaatacatc
    43441 tatagttgaa aatatggatg agtttagaaa agctttagaa gaaaagccag gattcataaa
    43501 gactatgtgg tgtggagatg cagaatgtga agctaagatt aaggaagaaa caggagcaac
    43561 tataagatgc ttaccttttg aacaagaaaa cttaggtcac aagtgtgtgt actgtggaaa
    43621 agaagcagat agtatggtag taatggcaaa agcttactaa aatacattta aataaaaact
    43681 ggggtagaaa gtatttaata ctttatccca gtttttttgt tttttatgag taataaaatg
    43741 tatttgtaac tatgtcataa gataagtcat agatacttat attaaaaaga atataataga
    43801 gaactatttt taataattac atattaatga aagcaaaata ttttgttata tagattttca
    43861 ataaagcaaa caataaatat aaatgcatat taataaggag tgcttttaat ttttagaaaa
    43921 taaatgactt aaaagaaaga tttatgaaat taaaactagg attttctgat ataataaaca
    43981 tgtgtatttt taaataaaaa ggatattatt attagatttg attaaaaata aacttgcaaa
    44041 acaatgtact agtttaatat tatagttata ttgaataaaa tatcaggagg aagtaaaaat
    44101 gaatgtaaga gtaaggtttg caccaagtcc aacaggattt gtacatatag gtagtttgag
    44161 aactgcttta tataactatt tatttgcaaa aaaaatgggc ggagaatata tattaagagt
    44221 ggaagataca gaccaaagta gattagtaga aggtgctata gagaatatgc ttaatgctat
    44281 gaaatgggct ggggtcaacc atagtgaagg tgttatatta gatgatagtg gaaaagtagt
    44341 acaaaaagga gaatatggtc catatattca atctcaaaga ttagatatat accaagaata
    44401 tataaagcaa cttttagata gtggaaaagc ttattactgt ttctgtacaa aagaaagact
    44461 ggatgaagta agagatgctc aaagagcagc aggagaaaca gctaaatatg atggtcattg
    44521 taagaatctt tctaaagaag aagtagaagc gaatataaaa gcaggaatac catatgttat
    44581 aagattaaga ttgccagaga atcatacaat taagtttaca gacttagtta gaggagatat
    44641 ggaatttaat actaatgact tagatgacca agtattaatg aaaacagatg gatttccaac
    44701 ttatcatttt gcagtagttg ttgatgacta tttgatgaaa attacacatg ttataagagg
    44761 agaagaatgg gtatcatcta ctccaaaaca tgtttattta tatgaagctt ttggatggga
    44821 agcacctgta tttgtgcatt taccaaatat acttaataaa gaaaagaaaa aattaagtaa
    44881 aagacaaggt gatgtagctg ttgaagactt taagaaaaaa ggatatttac cagagggatt
    44941 agtaaactat gtagcattag ttggttggtc tcctgaagat aatcaagaat tgtttacaat
    45001 ggaggaatta gaaaaagcct tctctgtaga aagagtatct aaaagtggtg gagtatttga
    45061 tactgaaaaa ttaaattggg ttaatcagca ctatataaaa gatggtgatg atgcttatct
    45121 aacagactta gcaataccat ttttaataga agatggattt ataacagaag aagaagctac
    45181 taataaatat gatttcttaa agagtatgat atcagtactt aaagagaaat tacaatatgt
    45241 aaaagaagtt actgaacatg caagtatatt ttttggagat aaaatagagg tagaaactga
    45301 agagggaaga gagttcttaa gattagaaca tatacctact ttaatagatg ctttgagaga
    45361 aaaaatagaa aaaacagagg tattaaatgc tgagtttgtt caagctatgc ttaaagaaat
    45421 acaaaaagag tacaaaataa aagggaaaaa tttatttatg ggctcaagaa taattttgac
    45481 agggcaaatg catggtccag atttaccaaa agtaatggag gttttaggaa aagaaacttg
    45541 cttaaataga atagcttatg taaaaaataa tatattatag tcataaaagt ccgctaattt
    45601 agaagcggac tttttttgtg ttagtatttg gtgaaaatat ctgcttaaga aaatgaaggt
    45661 atttaaggta ataaatttaa tatataaaaa gcaaagcaaa aatataaata aaatatgaat
    45721 aataaactaa taaaatatta agcatagaag aagggaatct attattttag tttgatttaa
    45781 ttattaaggg ggtaattact atgtatagta atattgatga tgttaaaaag gaattaaagg
    45841 agctatgctt agaatatgtt acaattttag aaaaattaaa agatgaaaaa atgattacag
    45901 aagaaacttt tgaaaagtgc tcttctcaaa aaaaaatatt tttagaagaa caatagagga
    45961 tggtaatagt attattatta ttatgtagtt tgaaagatag tacgaaaaat gatagtaata
    46021 ctattattta ttgcaatttt caactataat ataatatata atgttaagaa gataatttat
    46081 aaaaatacag tgaggtgatg agtgtgaaag tttataatac attgactaga acaaaagaag
    46141 aatttgttcc attagaagaa ggaaaggtaa aaatgtatgt gtgtggtcca acagtttata
    46201 actatataca cataggtaat gcaagaccat ttataatatt tgatactcta agaaggtatt
    46261 tagagtatag aggatatgat gtaacttatg tacaaaactt tacagatgtt gatgataaga
    46321 ttataaatag aagtcatgag gagggaatat ctcctgaaga agttgcggct aagtatataa
    46381 aagaatattt tgtagactgt gatgggctag gaataaaaag agctactgtt catcctcagg
    46441 ttacagataa tatacaacag attatagagt ttataaaaga gttggaagat aagggatatg
    46501 catatgcagt aaatggtgat gtatattttg atacaaataa gtttgaaggt tatggaaaac
    46561 tttcaggtca aaaacaagaa gatttagagg caggggctag aattgaggtt aatgaccaaa
    46621 aaagacatcc tatggacttt gttctttgga aggcaaaaaa agaaggtgag ccaggatggg
    46681 atagcccttg gggagaaggt agaccaggat ggcatataga atgttctgtt atgtctaaga
    46741 gatatttagg tgaaacaatt gatatacatg caggtgggca agatttaaca ttcccacatc
    46801 atgaaaatga aatagctcaa agtgaagcta gaagtggaaa aacattctca aaatactgga
    46861 tgcataatgg atatataaat ataaatgatg aaaagatgag taaatcaaaa ggtaatttct
    46921 ttacagtaag agatatatca aaattatatg atttagagat agtaagattt ttcatgttat
    46981 cagcacatta tagaaatcca gtaaacttta gtgatgagat gttaaatcaa gctaaagcag
    47041 gtcttgaaag actatataat actaaagaaa aattagaatt tacgctaagt aatttagtag
    47101 aatctccttt aacagaaaaa gaagtagagc ttgttaaaga attagatgac tttagacaaa
    47161 aattcataga tgctatggat gatgatgtta atacagcaga cgcagtaagt gtaatatttg
    47221 aattagctaa gttaataaat tctaatgtag atgaaaattc ttctttggag tttgctaaaa
    47281 aatgcttaga tgaatttaat gaacttacag gagtattaaa tattgtaaat aagaaaaagg
    47341 atactgtatt agataaagac attgaagaat tgatacaaaa aagaactgat gcaaagaaaa
    47401 ataaagagtt tcaattagct gatgatatta gacaacaatt actagatatg ggaattgtat
    47461 tagaagacac aagacaaggt gttaagtgga aaaggatata aaaatggaaa aaactgaatt
    47521 agttactatg tcaccattag ttttagcata cttaggtgat acagtgtatg agacatatat
    47581 aagagaatat ttaattagac agaatacaca aagaaaagtt aatgatttgc ataaactagc
    47641 tataaagtat gtaaaagcta aagctcaggc aactataatt catgaaattg aaattgagtt
    47701 aactgaagaa gagagtaaaa tttataaaag gggaagaaat caaaagtcaa atacttctcc
    47761 taagaatgca gatattatag actacaaaca tgcaactggg tttgaggcat tagttggata
    47821 tttgtattta aataatgaaa tagagagact tcaatatatt atcaataaag gaatcaaaat
    47881 tattgaaaga gatatgtaaa tattaaagag tgttgtctta ataataagta ataaggcaac
    47941 acttttttta taactaatgt ataataaata tatagaaatt agtaatgaga aaggataaaa
    48001 agttatgaaa gtaaaattaa tatcacatac acctgaacca gaaaaagtaa tagctatggc
    48061 agctaagcta tgttattcac cagttgggac agacgaaata gaaaaagact taacagatga
    48121 aagtatagag aaatttttaa atatgttatt aagtataggt catgggtcaa tattagaaca
    48181 tgcttcattt acattttcaa tagaaggtat ttcaagggct tgttcacatc aaattgttag
    48241 acatcgtata gctagttttt cacaacaaag tcaaagatat gttaaattag aacaatttga
    48301 gtacataatt cctccagaaa tagaaaaaat agaaaaggca aaagagttat ttatagactc
    48361 catgaaaaaa gaccaagaga attatgataa gttagttgaa atattgtttg aaaatcatta
    48421 taatgaccta ataaaaaacg gtaaaaatga gaaaacagct aaaagacaag ctgaaaagaa
    48481 agcaatagaa gatgctagat acgtatttcc aaatgcatgt gaaacaaaaa tggtatttac
    48541 tataaatgct agaagtttgt ttaatttctt tgaacataga tgctgtgaaa gagcacagtg
    48601 ggaaataaga aatttagctg tggagatgtt aagagaggtt aaaaaggttg ctcctatatt
    48661 atttaaaaag actggtccaa gttgtgtaaa tggaagttgt ccagaaggaa ctatgacttg
    48721 tggagatata gttcaagtta gagaaaaatt taaggccttg taggaggtga ataatttggc
    48781 gagtatagaa ggaagaaatc cggtaataga agcaataaaa agcgacagag aaatagataa
    48841 aatattaatt gcaaattcag ctaaagaagg ttcaattaag aaaataatag gaatggctaa
    48901 agataagaat ataattattc agtatgttga taaacataag ttagatgaag taagtacaag
    48961 tcattcacat caaggtgtaa tagcttatgc aagtgagtat aagtattatg aattagatga
    49021 gctaatagat ttagcaaaga ataaagatga agacccattt ttcattatat tagatgagat
    49081 aacagaccca cataatttgg gttcaataat aaggacagca gatgcagtag gagcacatgg
    49141 agttatcatt ccaaaaagaa ggtctgtaca cataactcct gtagttgcca aagcatctgc
    49201 tggagcagta gaatatatgc cagtttgcaa ggtaaccaat atagttaata caataaaaag
    49261 actcaaagaa gaagggctgt ggattgctgc agctgatatg gatggagaaa ctttttataa
    49321 acaaaatctt actggtcctc ttggtgtagt aattggaagt gaaggttttg gaatatctag
    49381 attggttaaa caaaactgtg actttatagt aaaaatgcca atgataggta atgttacatc
    49441 tttaaatgca tctgtagcag gtggaatact tttatatgag atattcagac aaagactgga
    49501 taaaagtaaa taggcatgaa aaaaaatata aatcattact taataataga tggctacaat
    49561 attataaatg cgtgggacaa tttgaaagaa ctggctaaag aggacttaga agattctcga
    49621 gaaaagctta ttgatgacat tatagaattt tctgagttta tgggttataa gactataata
    49681 gtttttgatg cttataatgt taaaaattct cgagaaaaag ttgaaaaaag aaaacacata
    49741 acaatagttt atacaagaga acaccaaaca gcagatagtt atatagagaa atttataact
    49801 tctttatcaa agtatgatga tgtaaaagta gccactaatg actatgcaga acagcaaatg
    49861 attttaggaa aaggtgctac tagaatgtct gcaagagaat taaagttaga gttagatcgt
    49921 tccaagaata aaatgaagga aaaaaatatc ggtttgagga aaaaaattca gcgaaattgg
    49981 ttggaggaaa ggttagataa agaaacattg tcgaaacttg agaacattcg tagaaagcgt
    50041 tgaaattact aggttcttta gactataata tacctataaa tttgttttat atatggggga
    50101 gatttacatg ttggtagcaa aagaaaaaag ttatgagtta gtggacaatt gtcagcagga
    50161 tgagtataac attgtattaa gagcaagtga aggggataaa atagcactag agtacattat
    50221 tactaaatac agaaattttg taaaggcaaa agcaaagtca tattttttaa ttggagcaga
    50281 taaagaagat ataatacaag aaggaatgat aggcctttat aaggcagtta gagattttga
    50341 tggaagtaag accaattcat ttaaatgttt tgcggagata tgtataacaa gacaaataat
    50401 aactgctata aaaactgcaa caagacaaaa acatatacct ttaaattcat atgtttcttt
    50461 gaataaacca atatatgatg aagagtcaga cagaacactt ttagatatta tagctacaag
    50521 tatagtaaca gatccagaag agcttataat tagtaaagaa gagttaaaaa atatagaatc
    50581 aaaaatgaac gaattgttaa gtgacttgga attagaagta ctagaattat atttaaatgg
    50641 aaagtcatac cagtttatag cggacaagct taaaagagat gtaaagtcaa tagataacgc
    50701 tttacaaagg gttaagagaa aattagaaaa acatcttgaa aacagaaatg attaatagta
    50761 aaatacttac cataagctta ttgtgagcaa aattaattta tatcgaaaga taagggagga
    50821 aattcaaaat ggctaaagct aaatacgaaa gaacaaaacc tcatgttaat atagggacaa
    50881 taggacacgt agaccacggt aaaactacat taacagcagc aataacaaaa acattatatg
    50941 acagatatca attaggagaa gcagtagatt tcgcaaacat agataaagct ccagaagaaa
    51001 gagaaagagg aatcacaata tcaacagcac acgttgagta tgaaacacca aatagacact
    51061 atgcacacgt tgactgccca ggacatgctg actacgttaa gaacatgata acaggagcag
    51121 cacaaatgga cggagcaata ttagtttgtt cagcaacaga tggaccaatg ccacaaacaa
    51181 gagagcatat actattatca agacaagttg gagtaccata tatagtagta ttcttaaaca
    51241 aatgtgacat ggtagatgat gaagagttat tagagttagt agagatggaa gtaagagatt
    51301 tattaacaga atatgatttc ccaggagatg acactccaat agtaagaggt tcagcattaa
    51361 t
//
LOCUS       DAGSRM010000019        50985 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.019, whole genome shotgun sequence.
ACCESSION   DAGSRM010000019 DAGSRM010000000
VERSION     DAGSRM010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 50985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50985
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.019"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(121..894)
                     /locus_tag="MJE33_001224"
     CDS             complement(121..894)
                     /locus_tag="MJE33_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HBY9647169.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKNL"
     gene            complement(894..1280)
                     /locus_tag="MJE33_001225"
     CDS             complement(894..1280)
                     /locus_tag="MJE33_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBY9647170.1"
                     /translation="MEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVAVSEACTN
                     AIKHSKDDVFNIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLGLFIIKTL
                     MDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            complement(1305..1622)
                     /locus_tag="MJE33_001226"
     CDS             complement(1305..1622)
                     /locus_tag="MJE33_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HBY9647171.1"
                     /translation="MSMNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHALIEKNM
                     LDVKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYIVNPKSNVRKIFTITGLDKIFK
                     VEG"
     gene            complement(1634..1894)
                     /locus_tag="MJE33_001227"
     CDS             complement(1634..1894)
                     /locus_tag="MJE33_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBY9647172.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            complement(1894..2190)
                     /locus_tag="MJE33_001228"
     CDS             complement(1894..2190)
                     /locus_tag="MJE33_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBY9647173.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="MJE33_001229"
     CDS             complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="MJE33_001229"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBY9647174.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDIAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKLGLIEKFKLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKDEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="MJE33_001230"
     CDS             complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="MJE33_001230"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBY9647175.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMVAEVYLNGKIYKQTYEKGLPTSKLEVVGESQ
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPSTARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPSKSEIFLVEGDSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            complement(6607..7722)
                     /gene="recF"
                     /locus_tag="MJE33_001231"
     CDS             complement(6607..7722)
                     /gene="recF"
                     /locus_tag="MJE33_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBY9647176.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRINKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRAFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKNIHVDEALLDVYDDTLAKYGSYIYILRRDFIKKIANISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEVSHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            complement(7740..7946)
                     /gene="yaaA"
                     /locus_tag="MJE33_001232"
     CDS             complement(7740..7946)
                     /gene="yaaA"
                     /locus_tag="MJE33_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBY9647177.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVEIEGTKIKVL"
     gene            complement(8083..9189)
                     /locus_tag="MJE33_001233"
     CDS             complement(8083..9189)
                     /locus_tag="MJE33_001233"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBY9647178.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            complement(9431..10753)
                     /gene="dnaA"
                     /locus_tag="MJE33_001234"
     CDS             complement(9431..10753)
                     /gene="dnaA"
                     /locus_tag="MJE33_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBY9647179.1"
                     /translation="MMDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILL
                     APSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYP
                     NLNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHH
                     IVSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERT
                     QEEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIA
                     ILRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEA
                     LKDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTD
                     LSLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            11409..11546
                     /gene="rpmH"
                     /locus_tag="MJE33_001235"
     CDS             11409..11546
                     /gene="rpmH"
                     /locus_tag="MJE33_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBY9647180.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            11624..11968
                     /gene="rnpA"
                     /locus_tag="MJE33_001236"
     CDS             11624..11968
                     /gene="rnpA"
                     /locus_tag="MJE33_001236"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBY9647181.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEAYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            12000..12251
                     /gene="yidD"
                     /locus_tag="MJE33_001237"
     CDS             12000..12251
                     /gene="yidD"
                     /locus_tag="MJE33_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBY9647182.1"
                     /translation="MNKIIYILKEISKYLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFVKGMYLTIRRILKCHPFHPGGYDPLK"
     gene            12283..12990
                     /locus_tag="MJE33_001238"
     CDS             12283..12990
                     /locus_tag="MJE33_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBY9647183.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYVLAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            12959..13588
                     /locus_tag="MJE33_001239"
     CDS             12959..13588
                     /locus_tag="MJE33_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HBY9647184.1"
                     /translation="MRKSLKLIKSKSKEEAISKAIAELNLKAEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKTKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            13700..15079
                     /gene="mnmE"
                     /locus_tag="MJE33_001240"
     CDS             13700..15079
                     /gene="mnmE"
                     /locus_tag="MJE33_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBY9647185.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGEKALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNIVDNENIIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKEKTDELKKDIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVEKSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            15177..17069
                     /gene="mnmG"
                     /locus_tag="MJE33_001241"
     CDS             15177..17069
                     /gene="mnmG"
                     /locus_tag="MJE33_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBY9647186.1"
                     /translation="MKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSIA
                     LMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQADK
                     FKYHEEMKKTLEDEPNLDIAMDEVVEILHEGNVVIGVGTKLGCSFKSKAVILATGVYL
                     NSKIYMGEVAFYEGPNALGYAKYLTDSLVELGLRMRRFKTGTPARVHRDSIDFSVMSL
                     QEGDEKVTPFSFMNENIEKKQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPRY
                     CPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLENC
                     KIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINAV
                     RKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLTQ
                     KGYDIGLVKKDRYERFLNKKAAVEKEFERLKNERVTPKEVNSLLEEKGATPIKVGISL
                     YEFLKRPEVTYELLEELGKGAGEDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLEN
                     KKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIYLEQIRRT
                     RGGKGE"
     gene            17062..17781
                     /gene="rsmG"
                     /locus_tag="MJE33_001242"
     CDS             17062..17781
                     /gene="rsmG"
                     /locus_tag="MJE33_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBY9647187.1"
                     /translation="MNNIELLKNGIEGFNIDTNDSMLEKFKIYREILVDWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDSLELTLLDS
                     LNKRINFLEEVSRVLELDNITFIHGRAEDFGKNEDYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEVDLNHNILVFKK
                     VMETPVKYPRKAGKPAKNPIK"
     gene            17944..18729
                     /gene="noc"
                     /locus_tag="MJE33_001243"
     CDS             17944..18729
                     /gene="noc"
                     /locus_tag="MJE33_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HBY9647188.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKVQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            18864..19637
                     /locus_tag="MJE33_001244"
     CDS             18864..19637
                     /locus_tag="MJE33_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBY9647189.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENVDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            19639..20502
                     /locus_tag="MJE33_001245"
     CDS             19639..20502
                     /locus_tag="MJE33_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBY9647190.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETSEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLSDSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            20535..21677
                     /locus_tag="MJE33_001246"
     CDS             20535..21677
                     /locus_tag="MJE33_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBY9647191.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKALEKRGIENTVVKCDYEGFLDYEDLEKSIKSNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYDIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVPDIKIYGPKDSKKRASVIALNIGDMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(21810..22385)
                     /locus_tag="MJE33_001247"
     CDS             complement(21810..22385)
                     /locus_tag="MJE33_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HBY9647192.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGSDSSKKETKSNTNVEQ
                     PKEENNTEKEVPNNKAKLTPKEETKAQSATIYSFDVDKTDLIENKVDLNKIDENTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAETLMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
     gene            22645..23937
                     /locus_tag="MJE33_001248"
     CDS             22645..23937
                     /locus_tag="MJE33_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBY9647193.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSENKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEVTLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            24042..24644
                     /gene="yedF"
                     /locus_tag="MJE33_001249"
     CDS             24042..24644
                     /gene="yedF"
                     /locus_tag="MJE33_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HBY9647194.1"
                     /translation="MSIKIDARGLACPKPVINTKRELDNLEEGVVVTIVDNETAKENI
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNVVDNKESELDNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILENMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            24717..24980
                     /locus_tag="MJE33_001250"
     CDS             24717..24980
                     /locus_tag="MJE33_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HBY9647195.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILVENNVDIKGIYCISKLENGSKEVEKLD"
     gene            24995..25180
                     /locus_tag="MJE33_001251"
     CDS             24995..25180
                     /locus_tag="MJE33_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBY9647196.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDIKIKCTKCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            25283..26479
                     /locus_tag="MJE33_001252"
     CDS             25283..26479
                     /locus_tag="MJE33_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBY9647197.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDSDGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLDKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            26693..26971
                     /locus_tag="MJE33_001253"
     CDS             26693..26971
                     /locus_tag="MJE33_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBY9647198.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            27008..27442
                     /gene="ssb"
                     /locus_tag="MJE33_001254"
     CDS             27008..27442
                     /gene="ssb"
                     /locus_tag="MJE33_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBY9647199.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            27461..27688
                     /locus_tag="MJE33_001255"
     CDS             27461..27688
                     /locus_tag="MJE33_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBY9647200.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            27831..28151
                     /locus_tag="MJE33_001256"
     CDS             27831..28151
                     /locus_tag="MJE33_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBY9647201.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            28159..29115
                     /locus_tag="MJE33_001257"
     CDS             28159..29115
                     /locus_tag="MJE33_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HBY9647202.1"
                     /translation="MNNKIRLSKAMSIIVLAIIIALVIAYIPMLGIFSMLAAVPYVII
                     GAITDRKYSLISILVTFCVLILFADISYALNICIMYSLPGIVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNSFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSKLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            29126..31120
                     /locus_tag="MJE33_001258"
     CDS             29126..31120
                     /locus_tag="MJE33_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBY9647203.1"
                     /translation="MSNKQTFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRRHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTANIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            31121..31570
                     /locus_tag="MJE33_001259"
     CDS             31121..31570
                     /locus_tag="MJE33_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBY9647204.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRSLGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            31589..32917
                     /gene="dnaB"
                     /locus_tag="MJE33_001260"
     CDS             31589..32917
                     /gene="dnaB"
                     /locus_tag="MJE33_001260"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBY9647205.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASVAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            33122..33430
                     /locus_tag="MJE33_001261"
     CDS             33122..33430
                     /locus_tag="MJE33_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647206.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKYEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            33512..34801
                     /locus_tag="MJE33_001262"
     CDS             33512..34801
                     /locus_tag="MJE33_001262"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBY9647207.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKTDNISTENIKISDRA
                     HVIFPYHKELDALSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNEIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAVRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYVAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            35127..35202
                     /locus_tag="MJE33_001263"
     tRNA            35127..35202
                     /locus_tag="MJE33_001263"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35160..35162,aa:Lys,seq:ttt)
     gene            35209..35283
                     /locus_tag="MJE33_001264"
     tRNA            35209..35283
                     /locus_tag="MJE33_001264"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35242..35244,aa:Glu,seq:ttc)
     gene            35288..35363
                     /locus_tag="MJE33_001265"
     tRNA            35288..35363
                     /locus_tag="MJE33_001265"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35321..35323,aa:Val,seq:tac)
     gene            35397..35471
                     /locus_tag="MJE33_001266"
     tRNA            35397..35471
                     /locus_tag="MJE33_001266"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35429..35431,aa:Thr,seq:tgt)
     gene            complement(35854..36846)
                     /locus_tag="MJE33_001267"
     CDS             complement(35854..36846)
                     /locus_tag="MJE33_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBY9647208.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSIDDE
                     ISKIGLSLAKIVLLNPKSKDEIVKKTKENIESLKVKKERLLAESNIPLDYLEIKFQCI
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKGSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDTNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
     gene            complement(36855..37913)
                     /locus_tag="MJE33_001268"
     CDS             complement(36855..37913)
                     /locus_tag="MJE33_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBY9647209.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNNSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNGEYDNFNYSIEFLDLK
                     NFYIENILSNNSSIKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIINACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            38038..39501
                     /locus_tag="MJE33_001269"
     CDS             38038..39501
                     /locus_tag="MJE33_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HBY9647210.1"
                     /translation="MVIKFNQKVRRTLSVAILFMVLIIGCFIEKDDSLETYKEKTNSK
                     INQYNMDVIFDDETKRLMCNQNVDYINNTKSNLDKIYFHIYPNAFSKKDFAPFEKSEM
                     TRAYPNGFNEGYIDIKNILNNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYN
                     VKIPNSIGRFGYGENTINVTNWFPIACVNDERGWNLKSYETIGDPFYSETSDFHVKLL
                     IPNKYKIAHTGKLIDDKHDNKKMLYEIEAENVRDFAFILSSKFNVNKVDSKGVAINTY
                     NLNKKLSKKATEVAKDSINIFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLY
                     NEKNEFLLEYVIAHETAHQWWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMAN
                     KLIKTMEIQTKSYLSENIFKPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFET
                     LNEYYNKYKHKNANGRAFVELWNSKGVDINKIISECK"
     gene            39603..40400
                     /locus_tag="MJE33_001270"
     CDS             39603..40400
                     /locus_tag="MJE33_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBY9647211.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDIG
                     VDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTESDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSSTGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDVSNVYEMK"
     gene            40413..42515
                     /locus_tag="MJE33_001271"
     CDS             40413..42515
                     /locus_tag="MJE33_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC/EAL
                     domain-containing protein"
                     /protein_id="HBY9647212.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     AYQEYLSGLFNGEVTNILNFINDSTLGIISLIILLTISYSYGKIYGSKYTVLVPIVAM
                     CSFLVFSHGNELNSYIEIFKTKWLFTSIIVSMTSSVLFVKLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVIMISLIGSSNFQDIFYNLFSNVFNKMGTNLMSAL
                     LFIFLMHFMWFFGIHGSNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGSLLCLVIAIFLSEKRINVRKLAKIASIPALFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTITSYVAMSTGIVPCTVSAVEWTSPIFLSGYMSTGSIRGSLLQVFNLCVG
                     VMIYIPFIKMSQNRYTYMFKNNIENLTNLLKRYELTGEQPNLLNHNGKIGSISKMLAS
                     DLKFAMKRGEIELFYQPQVNYNGNVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACIDLKKSEKLLRNPVKFSVNISPDQLDDPKLPEQIKNIISSNDINPNM
                     LGIEITEQIALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENIDQRDKLYGLGCEIYQGYYYS
                     KAIPFEEFVEYANQEREI"
     gene            42583..43062
                     /gene="rlmH"
                     /locus_tag="MJE33_001272"
     CDS             42583..43062
                     /gene="rlmH"
                     /locus_tag="MJE33_001272"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBY9647213.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLEIIELDDEKAP
                     ENLSVKEMEIIKDKEGKKILGKIKHNSYVIALAIDGKGLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSRMTFPHQMMRLILLEQIYRAYRINNGEPYHK"
     gene            43129..44820
                     /locus_tag="MJE33_001273"
     CDS             43129..44820
                     /locus_tag="MJE33_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647214.1"
                     /translation="MDRLDIFIENYDFKEYLEKLPRELIEENYKDFVIEFWKNPSVDI
                     IKLKSKYSIKNVVSISSILMQNPIQEYYEFLEDIWKNNIKIDELINKYLITATRMDQI
                     KFPLIYIDEDSFCPYCLGNKFNIINNIKQQDIKVECEKCGEAISYFIHKEEVDNIIEE
                     EKRKKELFIRTLENVYNKINKIKCHRCQSDLYVCINDKIMEYNIRCDRCNSQYESVEI
                     AQEQYRLWQQRAAMMANIKNKENELLLKTLSIKDEKDIKFKLEDIITESESIDVISDL
                     VDMQDRGENIFKQLFNNYVRLGRLDRRLLVTVCKDCKQNENTYTWERGKEHLINMRGI
                     TYSEAVVYELMEKTKLLPIRAIIRRLMEKKLLYCDERSNNLQVHPVLIENLAKLESIN
                     KSQNINNQVAYLIFNKQKYICYHCGESGRPLKIGYLTIDKNINDLSSLVGICDSCYED
                     ITEDEIIIDNIFCVVDERNNELGEAWKFLIQYDSSFRNNTIAEVEHARLYDMYDEDNL
                     IRAYANTLYKQTKDGFANEKVFFAYARAILKNSKGQVSVLQIAKEKFKLEKWLNG"
     gene            45052..46524
                     /locus_tag="MJE33_001274"
     CDS             45052..46524
                     /locus_tag="MJE33_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647215.1"
                     /translation="MSHSKLSDHKLKKGKFIAPFNEFMTPLNSNESWCLGRLPEYIWI
                     GLLINKYGRKKGMEIIYKIIVKLHSIESDIVTPRLSEILSMEDVKQDEFFSYILTIVE
                     KEALSPLTLIFTYSNYPIFVKYFAEPSIKIEDRVTYLDETLRKAMHHQSEFATDIRFI
                     VLYYFCMSGKLVLQKEQINNLLEYPNLEHTDEKMRMLRPFIRAAELMVLNMEKKSDWY
                     LDIFWGRISVMTECKLYATKFEDETANTDLYMEQLHEIFVYLSDLLCKVNPLDEKMLV
                     LLGIAIYSYKRLREVVEHKLFNSISGRGSVRVLIENYIMMKYLVKIEAQHENIWSEYQ
                     FYGIGQYKLIVARYREQEQDLKNSHVDYKYIEALVNEFVIEETIDMDTKYFDKQNIRS
                     KAEIVGEKELYGLYYDYDSAYEHGLWGSIRESSMLKCDNPAHHYHCIPDVDNNQNLKS
                     VWHDCVNIMEKTLLFLDTVYGIPQHLLLEVIKNGKEFFTK"
     gene            46499..47563
                     /locus_tag="MJE33_001275"
     CDS             46499..47563
                     /locus_tag="MJE33_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647216.1"
                     /translation="MEKNFLLNKINSIQKEYLELLVKLKDNIDTIDYIYIIDEINIFW
                     YSYRDVIKLYLGNITQKDNAYIFTGATYLDVDDLEHYPFVMMGDIHIVDDSLCKYSKT
                     VLVLSNQEFAKVLKDQIISCIDDNIKILSNYSFAMLILPMNLLYGNDMKIIHEYSNNI
                     FLNFFSRKDLNLDTYFEEFNSIEDIALALNPGIESYIVFDEGEDLTKNLEERFKSFID
                     KGKVPLPAHKSDAFKFWLIIYGFITQALDIILSCKGFNLTPYLRYRVTLQYVLLLGDN
                     VFDIEDLSNMLLKCKVAHVLYAIFDKSEIKNISFVQFYKKLSEYKFSYKVENDIKENI
                     NINQIAEILNKNITLFLNYI"
     gene            47703..48875
                     /locus_tag="MJE33_001276"
     CDS             47703..48875
                     /locus_tag="MJE33_001276"
                     /inference="COORDINATES: protein motif:HMM:NF013752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBY9647217.1"
                     /translation="MLKQNTIQIDYTTYGNNYQFKLPLNIDYMIPKDAIFVDGTKIES
                     SANRYTFVWKKTINKNMVRVMNDISNFILKCGIDFGIKIIYRNKIKIYHLKKLLKKLK
                     KISKESNITFVHGKGKKKSIVQKSIAKLNNYIDRLKKYTKDLHIMGELNSYSKTDNDA
                     TFMRMKKDHMKNGQLKPAYNIQFGVDSEYIVWVSSEHKPTDTTTSIPFLKSIEEHTKF
                     KYPKIVADSGYESEENYTFLECNNQLSFIKPANYEISKTRKYKNDIGRIDNMDYDEKK
                     DYYICKNGKKLFANKIINRKSKTGYKSKITCYTCKECCDCQYKNGCIKGNNSKIPLEN
                     RTKNLQVSKLFHEKRKENLKRIISQEGCQLRMNRIIQAEGTFAQVKQNMKFRRFLS"
     gene            49317..49628
                     /locus_tag="MJE33_001277"
     CDS             49317..49628
                     /locus_tag="MJE33_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY9647218.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIARPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            49676..49993
                     /locus_tag="MJE33_001278"
     CDS             49676..49993
                     /locus_tag="MJE33_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBY9647219.1"
                     /translation="MDEKIIEISFNIIGYAGEGKGLAFEAIKEAKNGNIEKAKELLKE
                     SREVINKAHRYQTELIQNEASGNRTEISVILIHAQDHLMNGMNFQQLAEEIVDLHLKL
                     QDK"
     gene            50206..50907
                     /locus_tag="MJE33_001279"
     CDS             50206..50907
                     /locus_tag="MJE33_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY9647220.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLSKKTIS
                     IEKIINDTNSQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNESSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLAKDILLGRKRKTYDENDNIVELSFA
                     RYNTEVQNYVIKYEI"
BASE COUNT        19565 a         6172 c         8107 g        17141 t
ORIGIN      
        1 atataaatta acctactgtt taattttcaa tgttcgtgtt tatttcttgt tccttttttc
       61 aaggacaagt aatactatac catctttaaa acagtaggtc aatacttttt ttatattttt
      121 ttataaattt ttttcatatt cttttttcag cttctctact acctttttct ctagtctaga
      181 aacagtcatc tgtgatatcc ctagttcttt agctatacta gattgtgttt tatcaaagaa
      241 aaatctatct ttaaaaattt taacttctaa ttcatttaaa gtttcaataa acttattaat
      301 aaaatcttca tactctatac ttccaaagtt accttcttct tttccaatct tatcaataag
      361 agttatatct ttatcctctg aatcatcatt acttgatgaa tccaaagaca ttggttgata
      421 tccataagaa gcctccatag cctctaaaac atcttcttca ctaacgccta tataatcagc
      481 tatatctttt acctttggat gcttcttatt ttcttgttct aatttaatct tcgcatcact
      541 tatctttttg cttaattcct gtattcttct tgggactcta agtgtccata ccttatctct
      601 aaaatacttt tttatttctc ctactatagt tggtgttgca aagctggaga actcaaatcc
      661 tttctcaaca tcatatctat caatagcata aattaatgcc aatgaagcaa cttgatatat
      721 atcttcaaaa tcaacacctt tgtttatata cttcttagct aacaatcttg ctaaatacag
      781 atgtctttca ataagaatat ttctaatgtc tacattttta tttttattat ataaattaaa
      841 tagctctttt gtatccatat ttaggtaatg tgtagcatta gctacatttt tcattaaata
      901 tcaactccta aatatttagt catttttatt tttgtccctt gattatgttc tgattcaata
      961 tctacatcat ccattaaagt tttaattata aacaatccaa gaccactctc ttttggattc
     1021 tctaaatctg gtgtacttat tgagctaaca tcatatcctt ttccattatc ttgaatttct
     1081 atattaattg cattatctaa aatattaaat actatattaa acacatcatc tttactgtgt
     1141 tttattgcat ttgtgcatgc ttcagatact gctaccttta tatcttctat atcatctatt
     1201 ggaaaaccca tcttattggc aattccagat gctgttaatc ttattatact aacataatct
     1261 ggatttgtag taatttccat ctttatagtc tcacaagcca aagtttatcc ctccacttta
     1321 aatattttat ctagacctgt tatagtaaaa atctttctta cgttactctt aggatttact
     1381 atgtatatct ctttttcatt tatttttaat ttttttaata cacctatcat agctcctaag
     1441 cctgttgagt ctatataatc taaatcttta agattaatct ttacatctaa catatttttt
     1501 tctattaaag catgtaaatg ttcctttagt ttatctgctg tagaaacatc aagttctcca
     1561 tcaagacaaa cattccaaaa tttattttgt gaatctaaat ttgaatcaat attcatagac
     1621 atgtaaaaac ctcctatttc cttttaataa ttttaaatat atttcctata ctcaatattt
     1681 tattaattac ataaactata tcatctattc cctttgatat tgatgatgca ttttcaacaa
     1741 tatcttgtat atgtctttca ttataatcta caatcttata tgccttttca acaaccttat
     1801 ttatgctgtt tagagtttta gctaaataaa ttgctactat aagagcagag cttcctacta
     1861 acactgctcc aatttgccac ccccatgcat tcattagtta acatctccct catcatcaaa
     1921 gcttttactt attactatat cttcttctga aatctttata tcttcttcat tattatcatc
     1981 atccactaaa tttattattc tctctttaac atcattaaat gtttcatgta gaacttcttt
     2041 tgggttttct ttgacatcat taaacttctc ttttgtttct tttcttagtt cagaaccttt
     2101 tttaggtgca aaaaacatac caaatataaa ccctaaaata gctcctaata ataaaaatcc
     2161 acctttttta caacttcttt tattgcacat aacaattact cctttcaatt ttaaattttt
     2221 atctaactac attatacaaa aagaatagga tatttatcct attcttcttc aatatttatt
     2281 ttggcaatcg aaactacatt gacttcatca ttcgtcttca ttaatttaac accacttgta
     2341 actcttccaa acagtgatat ttcattgact cttattctaa taagaactcc atcagaattt
     2401 ataatcatta tctcatcatc ttcgttgacc atatctgcac ctacaattgt accagttttt
     2461 tcagatatat tataagtttt aattccttta ccagctctta tttgacttct atactcttct
     2521 atatttgttc tctttccaaa accattctta cttacaacta acacatctgt acctttactg
     2581 caaaggttca tagacacaac aaaatcttct ttacttaaag ttataccttt tacacccatt
     2641 gctgttctac ccatatatct tatatcattt tcatcaaatc ttatagacat accttcttta
     2701 gttactaaaa gtacttcttg ttttccatct gtaagttcta ctccaataag ctcatcatca
     2761 tctcttaaac ctattgcaat aagaccagat ttatttatat ttttgaactc ttctctctta
     2821 gtctttttaa caataccttt tttcgttgca agtaataaat attcattttc atcattacca
     2881 tcaataggta ttagagtagc aattttctca tctgctgaca attgaagtaa attcactata
     2941 gcagtacctt tagcctgtct cttaccttct ggtatttcat atgcatttaa tttaaatact
     3001 ctacctttat ttgtaaagaa taatagtcta ctatgagtag ttgtagttac caagtgcctt
     3061 acaaaatctt cttctctagt tgtaagtgct gaaatacctc ttccacccct tttttgactc
     3121 ttataagtat cagatggaag cctttttata tatccaaagt gagtaagagt tattgctatt
     3181 tcttcatcac ttataagatc tctcatatct atttcgcctt cagcatgtct tatttctgtt
     3241 cttctctcat cagagtaatt ttcttttatt attgtaattt catcctttat tacatttaaa
     3301 agtaatcttt catcagctaa aatctctttt aatctattta tcttcttgat taaatcttca
     3361 tattcagctt ctatcttatc tctttctaaa cctgtaagtc tttgaagtct catatctaat
     3421 atagcttgtg cttggatttc agttaatttg aatttttcta ttaaacctag cttagcttct
     3481 tgcccagtct ttgaagctct tatcaagctt ataacagcat ctatattatc taaagcaatc
     3541 tttaatcctt ctaaaatatg tgctcttgct tcagctttat ttagttcaaa tttagttctt
     3601 cttgtaacaa catcttcttg atgtttaata taatgatata atatttgttt aagatttaaa
     3661 actcttggct gaccatctac aagtgcaagc attattatac taaaagtatc ttccatttga
     3721 gaatgtttat acaaattatt taatactata ttagcattag catccctctt taattctata
     3781 acaattctca taccatttct attactttcg tctcttaggt ctgatatacc ttctattctc
     3841 ttttctttaa ctaactctgc tattctttca acgagtttag ccttatttac ttgataaggt
     3901 atttctgtaa ctattatttg ctgttttcct tttggtagct cttctataaa agctctagac
     3961 ctaactttaa cttttcctct tccagttctg tatgcttctg ctatactttc ttttcccatt
     4021 ataattgcag ctgtagggaa atctggtcct tgaacaaatt ttattaaatc atctacacta
     4081 cactctggat tatctatcaa atatacagtt gcatcaatta cttctgctaa attatgtgga
     4141 ggtattgaag ttgccatacc aacagctata ccatttgagc catttactaa taaattagga
     4201 tatctagctg gcaatactga aggctctttt aacgactcat caaagtttgg tttaaagtct
     4261 acagtttcct tttcaatatc tctcagtagt tctaatgata atttactcat tttagcttct
     4321 gtataacgca tagcagctgg tgaatcccca tcaacagaac caaagttacc atgaccatct
     4381 actaaaagtg ctctagttga aaaatcttgt gccattctta ccatagcata ataaacagca
     4441 atatctccat gaggatggta cttacctaaa acgtccccaa caatacgagc tgacttccta
     4501 tatggtttat ctggagttaa atttaactca ctcattgaat ataatattct tctatgaact
     4561 ggctttaaac catctctaac atcaggaaga gcacgtccag ctataacact cattgaataa
     4621 tcaatatacg attttttcat ttcttccgct atttcaatag ggagtatttt gttattttct
     4681 tccatgtata ctaatcccct ctctctatat atctaagttt ctaacaaatc ttgcattttc
     4741 ttctataaat tcttttcttg gagcaacctt atcacccata agcattgaaa atacttcatc
     4801 tgcaattgca gcatcttcta cagtaacttg taataaagtt cttgtctcag gattcatagt
     4861 tgtttcccat aattgctctg cattcatctc tccaagacct ttgtaccttt gaagttcaac
     4921 accatttctt ccaatttcat ctaataaaat atttaactct ttatctgagt aaacataatg
     4981 ttccttcttt tgctttgtca ctttatataa aggtggctgt gctgcataaa catacccctc
     5041 atctataaga ggtctcatat atctaaagaa gaatgttaat aataaagttc ttatatgggc
     5101 tccgtctaca tcagcatcgg tcataattat aattttatga tacctagcct tatctatatc
     5161 aaaatcttct ccaataccac aaccataagc tgttatcata ttttttattt catctgaaga
     5221 tagtatccta tctagtcttg atttctcaac attaagtatt ttccctctta atggaagtat
     5281 agcttgacta tttctatctc taccttgttt agctgaacct cccgctgaat ccccttcgac
     5341 taagaatatt tcacttttag atggatcttt ttctgcacaa tctgcaagtt ttccaggtaa
     5401 agatgtactt tctaatacac tttttctcct tgttaattct cttgcttttt ttgcagcttc
     5461 tctagctctt tgtgctctta aagctttatc aactatgatt cttgctgttg atgggttttc
     5521 ctctaggaaa gaccccagtt catcaactgt tacactatca actatacctc tcataaagct
     5581 attacctagc tttgttttag tttgaccttc aaattgaggt tctggtaatt taactgatac
     5641 tactgctgta agaccttctc ttatgtcttc accaagtagg ttaacatcat tttcctttaa
     5701 aaatttattt ctcttagcat aatcatttac agttttagta agagctgctt taaatccact
     5761 taaatgagaa ccaccttcgt gtgtatttat attgtttgca aaagaatata tgttctctgt
     5821 ataaccatct gtatattgca tagctagttc aacaaaacaa tcatcaacct ttttatctat
     5881 ataaactatg tcatcatgta taccagttct agatttattt aaatacttaa tgtattctac
     5941 caaaccacct gtatagtgaa attcttttct tttttcttgt ccttctcttt tatcttcaaa
     6001 tactattttt attcctttat tcaagaaaga aagttctcta agcctatact ccaatgtttc
     6061 atatttaaac tctatttcat caaatattgt ctcatctggc ataaattgaa tcattgttcc
     6121 tttatcttga gattctccaa ctacttcaag cttagatgtt ggcaatcctt tctcatatgt
     6181 ctgcttatat atctttccgt ttaaataaac ctctgctacc atccattttg acaatgcatt
     6241 aactacagaa acaccaactc catgaagacc tccagatact ttatatcctc cgcctccaaa
     6301 ctttcctcct gcatgaagat tagttaatac tgtttctaaa gttgatttac cagttttagg
     6361 atgtatttca acaggtatac ctcttccgtt atcttttact aaaacactgc catcttcatt
     6421 aatagataca tatatgtctg aacaatatcc ttgtaaagcc tcatctatac tattatctac
     6481 aacttcataa actaagtgat gtaaacctct agggcttgta ctaccaatat acatacctgg
     6541 tctttttcta acggcttcta atccctctaa tacttgtatc tgacttgcac cgtattcttg
     6601 tttcatttaa tttcctccat tctctatgct aataacatcg ccattttcaa tattaaatat
     6661 tgtaacattt ttttcatcaa atattttttt atgtgaaact tcagcacttg ttataaacat
     6721 ttgaacattg cttaaattat ttactaaaag tttttgtctt gcttcatcta actcactaaa
     6781 aacatcatct aaaattaata ctggatattc ttcaacttca ttttttatta attctatctc
     6841 tgataacttt aatgatattg aagcagttct ttgttgacct tgtgaaccaa aaagtcttgc
     6901 atctaaatca tttatgaata tatttaagtc atctttgtgt ataccatatc tggtagtctt
     6961 agattctata tcattaggtc tattcgatga caacttggct aaaaatttat tatatactgt
     7021 atctatagta tcttcatcag ttatatttat ttgattttta tatcgaattg ataatctttc
     7081 aactccatta gtcaaattca tatgcatatt ttcagatata ttagctatct tttttataaa
     7141 gtctctacgc aaaatataaa tataacttcc atactttgct aacgtatcat catatacatc
     7201 taataaagct tcatcaacat gtatattttt aagcacccta ctcctttgag aaagtgtttt
     7261 attataattg gtaagatatt tataatattt aggtataatc tgactaatct ctttgtctat
     7321 aaatgccctt ctttctttag gaccctcttt aaccaacctt aaatcctcag gagaaaatat
     7381 aacaacattt aagttgccaa gtagctcttg tatcttttgt aaaggaactt tatttattct
     7441 tatccctttt ttatcttttc caataattag ctctattaga ctatatttat tatattttga
     7501 aaaacttcct ccaatatata agttttcact attaaatcta accatttctt tatctttgtt
     7561 agtcctaaat gattttccaa aagaaagcat ataaatagat tctactatat ttgtctttcc
     7621 ttgaccattt ttaccaacaa gtagatttac tttcccatta aattccagat gcaacttttt
     7681 ataatttcta aaattaacaa gttgcaaact tttaagtttc acaaaaagtt ctcctttttt
     7741 tacaatactt ttattttagt tccttctatt tcaactatat ctcctgattt tatctttttt
     7801 cctcttctta attctatttc atcatttact gtcactaaac cttcttgaat taaaaatttt
     7861 gcatgacctc ctgttgatgc aatctctgct aatttaagaa attgatccaa ctttatatat
     7921 tctgattcta tagttatttc agtcataata gttcctcctt gtcactattt taaacaaatt
     7981 cctattgata cactaaaagt atattatacc ataaatatgc ttataaaagg agttgtctaa
     8041 gattagaaaa attttctaca aaagacaact ccttcatgtc tgctatatat ttgatgatat
     8101 tcttactgga agcagtaagt aaatataatt aacatcatct gttggtttaa ttatacaagg
     8161 atttacattt gtagtaaatt ctataaatat ttcctcatta tctatatttt tcaaaccttc
     8221 gataaaatat ctagaattga aagctatatc taggtaatct ccatctaaat ctatttcaac
     8281 ttcttcatac acatttcctt tttctgtatt agaagtaatt gccattactt tatctcttat
     8341 agataacttt ataagattat ttttttctga ttgagacaat aaagacgctc tctctatact
     8401 atttaaaagt tccttagtat ttaatttaac tctgctgtta tgttctcttg gcaataattt
     8461 tttataatca ataaaatctc cttcaagcaa tcttgtaata attttcgtat cattaattat
     8521 aaaaatagca tttttttcgt taaatccaac tttaacatta tcctctccag aaagcaaact
     8581 atttacatct ataagtgtct ttccaggaat tatgactttt atattttcag ttaaactatc
     8641 aaccgaacaa ctttttacag ctaatctata tccatcaatt gctactaaat ttaagtttct
     8701 atctacaatt tctaaaagtt ctcccattaa aacaggtttt gtttggtcct gtgagatagc
     8761 aaatacagtt tgtttaatca tattttttag gatttcttga ggtatgctat ataaatcttc
     8821 ttcatttaat tctggtaatt taggaaattc ttttgctgca taacctttaa ttttaaatct
     8881 tgaatttaca caatttatgt aaatattatt ttctgaatca gtttctatct caacaaagga
     8941 atctggtaat tttctaatta tatctccaaa tagccttgca tctacaacaa catcaccttt
     9001 ttctatgatt tctgcttgta cataagtttc tattcctatt tcagcatcat atccagtaag
     9061 ttttaattga ccttcttctg tagatataag tatacctttt aataattcta ttgttgtttt
     9121 tccattaata gctttttgag caattccaat tctatttgct aggatttttt gattacaaat
     9181 tattttcaat gtggataacc tcctctggct ttttatatat aaaaataagt taaaatcata
     9241 gtagtaatag tagtaggtgt tgttgataat gttaataagt atgtataaca ttataaatac
     9301 aaagatatta acatgtgtat aagatgttaa tatctttgtt attttagact tgataatttg
     9361 ttaataattt ttgttcaaaa aaagtattaa caggttattc aagagatata aacatacaat
     9421 tgtgtataaa ttatcccttc aaatctgata taattttgtc tattttagtc ttaatttctt
     9481 cactttcttc tatatcctta gatactttgt catgagcatg aattactgtt gtatggtctc
     9541 tacccccaaa ctcttcacct attttaggaa gggataaatc agtaagctcg cgcgttaaat
     9601 acatagctat ctgtcttgga taagctattg aacgtgttct ttttttagaa ttaaaatctt
     9661 ccatctttaa attgaataca gaagatactt tttcttttat tctgagaaca tttatttctt
     9721 catttttaga agttgttata atatctttta aagcttctgt agcaagatca aaagatatta
     9781 ccctattgct aagagaagaa taagcaacca ctctagttaa tgcaccttct agttccctta
     9841 tgtttgactt aatatttttt gctatatatg acataacctc atttggcaca tctatacgtt
     9901 ctagttgagc cttttttcta agtatagcaa ttcttgtctc aaagtcaggt gcttgaatat
     9961 cagttataag tcccatttca aatctcgatc ttagcctatc ttctagtgta ggtatgtctt
    10021 ttggcggtct atcactagat ataattattt gtttgtttgc ttcatgaaga gtattgaatg
    10081 tatggaaaaa ctcttcttga gttctttctt ttcctgctat aaattgaata tcgtcgatta
    10141 gtaatacatc tacgtttcta tatttattcc taaattcttc gtttttatcg tcttttatag
    10201 agtttataag ttcattagta aatttttctg aagaaacgta aactactttt gagtcttttt
    10261 tttgactaac aatatgatga cctatagcat gcattaggtg agtcttacct aatccaactc
    10321 ctccatacaa aaaaagtggg ttataagctt tagctggaga ttctgcaact gcaacgcatg
    10381 cagcatgtgc aaatctattg ctattaccaa taacgaatgt atcaaatgtg tattttggat
    10441 ttagattggg gtaaagaact ctataattta aatctgtgct atcagaattt aaatcagcaa
    10501 cctctttttc gctcaataca aatttaatat tatatttttt taatgacagt tgactaatag
    10561 catcttcaat taaatgtaaa tatctattct ctaagatatc tttattaaaa tcactaggag
    10621 ccagtaagat taaatcatta agatgtattt ttaatggtac gatgttttta aaaaaggtat
    10681 taaaacttac tgaagttaag tcacctttta ttaattgtag ggttttgtcc cataaagaaa
    10741 ctatatccat aatataaacc tccgaacaga ataattaata ttttccacaa agttattaac
    10801 agaaaagcaa aaatatatat aaattttaca gaataactaa atttatggat aatggtgttg
    10861 ataaattaat aatagtataa attattaaaa aaatatgaaa agatataaac aatatgtaga
    10921 taaagttata aataacataa acatacaata aatggtgatt ataactagag tattgtcagt
    10981 aatcttaaaa gttatacaca aaagtatcca caagctgtga ataatttcta ataagttgat
    11041 aacatttatg cacattatca acaagtatat ttataatatc aaaatatatg tcaaacttca
    11101 atgatttgta actaatttat ccacaatatt aactaattgt tgataaaaat ataaacatgt
    11161 taaaattact ttatttgtgt atataattga aaaaaataat ttagaataaa attaaataga
    11221 tttttgctag tattttcaat aaaaaaaata cataatatat atatggtaga ttttgtgata
    11281 taagcgtccc aaaaactcgt tgacaacagt agtaaaagta tttataatta tatgtgttat
    11341 gtttactttg agggtaaagt tattattttt ataaacggaa aataaaatct gaaaggcggt
    11401 gcaaaataat gagtaaaaga acttatcaac caaaaaagag acaaagaagt aaagaacatg
    11461 gtttcagaaa aagaatgaag acgtctaacg gaagaaacgt gttaaagaga agaagagcta
    11521 aaggaagaaa tagattaact cattaattac aaaagggccg ctgcaaaggt ggcctttttg
    11581 tgtaaataat tacaattcat agctgtgttt atggaggtta actatggact ttaataggac
    11641 taaagggttg aaaaaagact ctgattttag aaaagtatat aaacacggca aatcttttgc
    11701 aaacaaatat ttagtaatat atatacttaa aaataaatca gattatagta gagtaggtat
    11761 ttctgtttca aaaaaagtag gaaaagctat tactaggaat agagtaagaa gattaataaa
    11821 agaagcctac agattaaata ttgatgaaaa aattaagcct ggatatgata tagtatttat
    11881 agcaagggta tctagtaagg atgctacttt taaagacata gacaagtcta taaaaaattt
    11941 agttaaaaga acggatattt ctatttaatt gtatgctata aatatggagg ttaatgactt
    12001 tgaataaaat aatttatata ttaaaggaaa taagtaagta tttatcgaat ttatgtattt
    12061 atttagtgag attttatcaa aagtatatat ctcccttaaa gggacctacc tgtagatttt
    12121 atccaacttg ctcacaatat tcaatagagg cgtttaagaa atatggattt gtaaaaggta
    12181 tgtatttaac gataagaaga attttaaaat gtcatccttt tcatccagga ggttacgacc
    12241 ctctaaaata aaagagagta actattagga ggtataaaaa aattgaatat aataggaaat
    12301 ttcttaggat tgattcttaa ggtaatattt gaatttgtta atcattatgg tatttctata
    12361 atacttttta cgatacttgt aaagattatt ttattaccat taacaattaa gcagactaag
    12421 tcaacaaagg caatgcaaga tatacaacct cgtataaaag agatacaaga aaaatataaa
    12481 aataagccag aaaaacaaaa tgaagaaatt gttaagttat atggagaagc taaaattaat
    12541 ccactatctg gttgtttacc tttattaata cagtttccta tattaatagg attatttagc
    12601 gttttgagag aaccagttgc tcatggcgtt tttgcaaaca aagctgcatt tttagcagct
    12661 gacaatgggt ttttatggat aaaaagctta acatcaccag attatgtatt agcagtattt
    12721 tcaggagcaa gtgcatatgt catgcaaaaa gtaatgacac caaaagatca gttacaaggc
    12781 tctatgaaag taatgacata tgtaatggca ggtatgtctt tctattgggg atttatattc
    12841 ccagcaggac ttacattgta ctggacagta agtaacttat tctcaatagc tcaatattac
    12901 ttaataatga atcctttaaa agctaaactg gctgctaact ctaaggagga aaaagttaat
    12961 gagaaaaagc ctaaaattaa taaaaagtaa gagtaaagag gaagctataa gtaaagctat
    13021 agcagagttg aatttaaaag cagaggatat agaagtagaa atacttgaaa atccaagtaa
    13081 agggttttta ggttttatag gtgctaaaga tggtacttat gaaatttttg ttatcgagag
    13141 ggaagtagat gtagctaaaa attttataga ggtaatgttg aaaaatgcaa atgttgatgc
    13201 caaagtcaat gtatctcaaa aagataattt aatcatggtt gatattgaag gcaaagaagc
    13261 tgcaagttta attggtagaa gaggggaaac tttagattct attcaatttt tgactggatt
    13321 agctcttaat aaaataaata aagattctca tacaagagta cttgtagata tagaaaatta
    13381 tagatcaaaa cgagaagaat ctttaataag atatgctaat aaagttgcta gagaagtagc
    13441 gaaaacaaga aaaactaaaa aattagatta tatgaatcca tatgagagaa gaataataca
    13501 ttcagctctt caaaatgaca aatatgtaat tacatatagt gaaggaaccg atccatatag
    13561 aagattagta attgagtgta aaagataaag tcaacctcct aaacattagg gggttttatt
    13621 ttttaatatt attaaatcat taatgattgg ttaaatgtaa atctatatgt aaaaaattat
    13681 gaattaagag gtgataaatt tgtttataga tgatacaatt gcagcaatag ctacagcacc
    13741 tggagaaggt ggtataggta tacttagaat aagtggagaa aaagctttga aggttgcaga
    13801 agaaatcttt aaatctatgt ctggaaagag tattgaagaa tacaataaga gaacattaat
    13861 atatggaaat atagtggata atgaaaatat aattgatgaa gttttattgg catatatgaa
    13921 aggaccaaat tcctatacag gagaagatgt tatagaaatt aattgccatg gtggatttat
    13981 ttcggtaaag aagatattag aattaatact atcaaaagat gttagattag cagaggctgg
    14041 tgagtttact aaaagagcat tcttaaatgg aagaatagat ttatctcaag cagaggctgt
    14101 aattgatgtt ataaaagcca aaactgatat agctcatgaa gttgctcaaa atcaattaga
    14161 aggttcttta tcaaagaaga taagagaatt gagagataaa gtcactgaga tattagctca
    14221 tgtagaagtt gctatagatt atcctgagga agatattgag catataacat atcaaactct
    14281 taaagaaaaa actgatgaac ttaaaaaaga cattaaaaaa ttatatgata cagctgaaag
    14341 tggaaaaata cttagagaag gcttaaagac ggtaattgtt gggaaaccaa atgtaggtaa
    14401 atcttcacta cttaattcta ttttaggaga gaatagagca atagttactg atataccagg
    14461 aactactaga gatgttatag aagagtttgt aaacataaaa ggtatacctt taaaaattgt
    14521 tgacacagca ggtataagag atactgatga tattgttgaa aaaataggtg ttgaaaagtc
    14581 taaagaatca tttactagtg cagatttgat agtaatggtt ttagatgcat ctagaaagtt
    14641 gtcagaagaa gatatagaga tattagaaaa acttaaggat aaacaaacaa tagtgctgtt
    14701 aaataagaat gatttgaaac aagagattga agaagaaaaa atacttaaat atgtagaaaa
    14761 taatagtata ataaaaatat cagctttaca acaagaagga atagaagaat tgcaagataa
    14821 aatagagtct atggtatata aaggtagtat caaaaataat tctagtttag tagtaacaaa
    14881 ttcaagacat aaagatgcac ttagtaaagc atataaatct gcaacagatg ctttaattgc
    14941 tttggaacaa agtatgccat ttgactttgt agaagtcgat ttaaaaaata tatgggacta
    15001 tcttggatat attaatggtg atacagtaac agaggattta ctagataata tattccataa
    15061 tttctgtatt ggtaagtaat gtacaaaagt aaacttgttt ttattataga gatttttaaa
    15121 aaatttgaag tgtttctcat gaaacattaa ttttgaaaag aaaggaatta taaatgataa
    15181 agtttgaagc aggaaaatat gatgttatag tagtcggagc aggacatgct ggatgtgaag
    15241 ctgctttggc tactgcaaga atgggatata aaactttgat aataacaatg tctttggatt
    15301 ccatagcatt aatgccttgt aatccatcaa ttggagggac tggtaaaggt caattagtta
    15361 aagaaataga tgccttaggc ggtcagatgg gattaaatat agacaagaca tatatacaaa
    15421 gtagaatgtt aaatactgcc aaaggtccag cggtacactc tttaagagca caagcagata
    15481 aatttaaata tcatgaagaa atgaaaaaga cattagaaga tgaaccaaat cttgatatag
    15541 ctatggatga agttgtagaa atacttcatg aaggaaatgt tgtaatagga gttggaacta
    15601 aactaggatg ttcatttaaa tctaaagctg taatattggc cactggagtt tatctgaact
    15661 caaagatata tatgggtgaa gttgcatttt atgaaggtcc taatgcattg gggtatgcaa
    15721 aatatcttac agatagctta gttgagcttg gccttagaat gagaagattt aagacgggaa
    15781 ctccagcaag agttcataga gatagtatag acttctctgt aatgtcttta caagagggtg
    15841 atgaaaaagt aactccattc tcatttatga atgaaaatat agaaaaaaaa caagaacctt
    15901 gttatttaac aagaactaca gaagaaactc aaaaagttat actggataat ttaaaaaggt
    15961 ctgctatgta tagtggagta atagaaagta ctggtccaag atactgtcct tctatagaag
    16021 ataaagtagt aagatttagt gataaaacat cacatcaatt atttatagaa ccagaagggt
    16081 taaatactaa agaaatgtat atacaaggaa tttcaactag tttacctttt gaagttcaat
    16141 tagatatgta caaaactata aagggacttg aaaattgtaa gataatgaga cctgcatatg
    16201 ctattgaata tgattgtgtt gacccaactc aactaaaaat aagtttggaa ataaagggtg
    16261 ttgagaactt atttagtgca gggcaattta atggaacttc tggatacgag gaagcagcag
    16321 ctcaagggct tatggctggt ataaatgcag ttagaaagat agaaggaaaa gagccatttg
    16381 tattagaccg ttcagaagct tatataggtg tcttattaga tgaccttgtt actaaaggaa
    16441 ctaacgagcc ttacagaatg atgacatcaa gagcagaata tcgactatat ttaagacaag
    16501 ataatgcaga tatgagatta actcaaaaag gttatgatat aggattagtt aaaaaagata
    16561 gatatgaaag gttcctaaat aaaaaggctg ctgtagagaa agaatttgaa agactaaaaa
    16621 atgaaagagt tacaccaaaa gaagtaaata gtttgttaga agaaaaagga gcaacaccaa
    16681 taaaggttgg aatatctcta tatgaatttt taaagagacc agaggttact tacgaattgc
    16741 ttgaagaatt agggaaaggc gctggagaag atgtttcaag agaagttaaa gaacagtgtg
    16801 taataataac taaatatgaa ggttatatag agaaacaatt gaaacaaata gaccaattta
    16861 aaaaacttga gaataaaaag ctggatgaga aaataaatta ttcatctata gaaggtctta
    16921 gattagaggc tagacagaaa ctagatgata taaaacctat ttcaataggg caagcatctc
    16981 gtatatctgg tgtttctcca gctgatatat cagttttact tatataccta gaacaaatca
    17041 gaagaactag aggtggcaaa ggtgaataat atagaacttc tgaaaaacgg gattgaaggt
    17101 tttaatatag atacgaatga cagtatgtta gaaaaattta aaatctatag agagatatta
    17161 gttgactgga ataaaaagat gaatcttact ggaatagaag atgaaaaaga agtgtatata
    17221 aaacattttt tagactctat ttcagctgta aagaatggat atataaaaaa tgggatgtct
    17281 ataatagatg ttggtacagg tgctggtttt cctggaatac cattgaaaat atgtttagat
    17341 agcttagaac ttactttgtt agattcgtta aataaaagaa taaacttttt agaggaagtt
    17401 agtagagtac tagagttaga taatattaca tttatacatg gaagagctga agattttggt
    17461 aaaaatgaag actataggga aaaatatgat gttgctacgg caagagctgt tgctggatta
    17521 ccgattctta tggaattttg tgtaccattt gtaaaagttg gagggtattt tatttgctta
    17581 aaaggtccga atgctaattt agagctagaa gaatcacaaa aggctattga tgttttagga
    17641 ttggagtata ttgaaaaaat agatgtagag ttaccagagg tagatttaaa tcataatata
    17701 ttagttttta aaaaagtgat ggaaacacca gttaagtacc ctagaaaagc tggtaaacca
    17761 gccaaaaatc ctattaagta atgaaaaatt tattattggt aagcttaatt atttctcagg
    17821 aaataattaa gcttactgta aaaaaacaaa aataaaggaa ttattaaagt agatgcagaa
    17881 ataaatttgt atgtacatat agagaacaaa aataatttat atctaaaata agagaggtat
    17941 gttatggaag ataaaagagt tatggagata cctatagaag atgtagttcc aaatccatat
    18001 caaccaagaa aaatattttc acaagtttca ttggaggaat tgagcaattc tataaaggta
    18061 tatggtataa ttcaacctat aacagttaga gcaaaagatg gcaaatatga actaattgca
    18121 ggagaaagaa gattaagagc agctaaatta gcagagctta aaaccatacc agcaattata
    18181 aataatatga acgatgaatc ttctgctgtt ctagcgttat tagaaaattt gcaacgagaa
    18241 gacttaaatt tcattgaaga agcaattggt tatgaaaact taataaaaga acatgctttt
    18301 actcaacagc aattagcaga aaaattgggc aaaaatcaat ctactgtagc aaataagctt
    18361 agaatattaa agcttccaaa cgatataaaa atgaagctaa tagagaataa tttaacagaa
    18421 agacatgcta gagcatttct taaactacca agtgaagatt taatgcaaag tgtgttagac
    18481 aaagtcataa aaaatgaatt gactgttaaa aaaacagaaa aattgataca agatgtatta
    18541 gaagaaacta aagttcaaga agagcctgat aaaaaacaga atataaaggg tgctatgagt
    18601 ataagaattt acataaatac tataaagcag gcctttgatg ctatatcaaa tacaggaatt
    18661 gatgctaagt ataatgaaat agataaggga gattatatgg aagttgttgt aaaaatccct
    18721 aaaaaataga gattactgct taccagtaat ctttttttgt aatataagcc ttgtaatata
    18781 aattgattta tagacattat ttatacttaa aaaacaccta ttcaacattt aaaattaatt
    18841 taattcttaa aggggggaac atcatgggca aagttatagc tgtattcaat caaaagggtg
    18901 gagtcgggaa aactacaaca aatgtaaatt taagtgctag cttaggaact ttgggaaaga
    18961 aaatattagt attagattta gacccacaag gaaatacaac tagtggatat ggtattaata
    19021 aaaacgaagt agaaaataca atctatgaaa ttatgttaga tgggttacat attaaagaag
    19081 caataataag tactgaattt gagaatgtag atgtagttcc atcagctact gaactatcag
    19141 gtgcagagat agagcttaca tctaaaacta atagagagta tattttaaaa aattccataa
    19201 aagcggtaat agatgaatat gattatatat ttttagattg tccaccctca ctggggatgc
    19261 ttacaataaa ttgtttaact gctgtagata gtgttttaat tccaatacaa tgtgaatatt
    19321 atgctttaga aggtgttagt caattaatgg agactataaa attagtaaaa tcaagattaa
    19381 atgctgatat agaaattcaa ggtgttgttt taagtatgtt tgatggaaga gctaatttat
    19441 caatacaagt agttgaagaa gttaagaaat actttaaagg tagtgtatat acaactttaa
    19501 ttccaagaaa tgtaagactt gcagaagctc caagtcatgg aaaacctgta atttattatg
    19561 ataaaagatg tagagggtct gtagcttatt tggagctagc agaagaattt atagatttag
    19621 aagaggagga atggtagtat ggagaataag tctaaaagaa gtaatagatt aggtagaggt
    19681 cttagtgcat tgataccaga aattaaagga gaaacttcag aaaaagaaat agtcaacata
    19741 gatatagaca aaatatatcc aaacgaagtt caacctagaa agcaatttga tgaagaaaag
    19801 ataaaagtat tgtcggattc tataaaaaat tatggtgttt tacagccaat agtggttaaa
    19861 atggatgaaa ataataaata tatgataata gctggagaaa gaagatttag agcatcaaaa
    19921 cttgcaaata aaaatcaaat acctgctata attaaagata tagatatgaa agatataatg
    19981 gagatagcct taattgaaaa cttacagagg gaagatttaa attcaataga agaggctcta
    20041 gcatataaga gtttaataga gcattacaat gtaactcagg aagaaatatc agaagctgtt
    20101 gggaagagta gacctcatat aactaataca ctaagacttt taaatcttgg acaagatgtt
    20161 atagaaatga tagacagtgg tagaatcaca gcaggccatg gtaaggcttt attgagaatt
    20221 gcagataaag atttgcaact tcaaatagcg aaaaaaatag aagaagaaga actttctgta
    20281 agagaagtcg aaaatatagc taaaaaaata tctgaaaata aacaagaaga acctaaaaaa
    20341 tctaaaccta aggatgtatt tattttagat gttgaagata aacttagaaa tatatttgga
    20401 acaaaggtga atatttctaa aggaaagaaa aaaggtaaaa tagagataga atactataat
    20461 gatgatgatt taaatagtat tgtatcaatg cttcttgaat agaacaaaaa tataaaacag
    20521 agaggaagat tgttatgatt tatttagata atgctgcaac tacatatcca aaaccagaaa
    20581 gagtgtataa tgctgtttta gattgtatga aaaattattg tgcaaatcca ggtagagcag
    20641 gccataaact tgctatgaga gctgctaggg agatatatga tacaagagag aatatagcta
    20701 agttgttcaa tgtaagtaat cctatgaata tagtatttac atctaatgca acagattctt
    20761 taaatttagc gataaaaggt gtattacaag aaggagacca tgtaataaca acgtctatgg
    20821 agcataattc agtaataaga cctataaaag cattggaaaa aaggggtatt gaaaatactg
    20881 tagtaaaatg cgattatgag ggatttttag attatgaaga tttagaaaaa tcaataaaga
    20941 gtaatactaa actgattgta acaacacatg cgtctaatgt atgtggaact ttaatagata
    21001 taaaaaaagt tggagaaata gctaaaaaac ataatatact atttttagta gatgcatcac
    21061 aaacagcagg tgtttatgat atagacgtaa atgaatgtaa tatagatatg ttggcaatgc
    21121 caggtcataa atgcttattt ggacctcaag gaacaggtat attatatgta agagaagggt
    21181 tgaacttaaa tatattaaaa gaaggtggaa caggtagtaa atcagaagaa atagttcaac
    21241 cagagttatt cccagataaa tatgaatcag gaactcataa tactccaggt atagcaggtc
    21301 ttaaccaagg tatactattt atatttgaaa gaggaatcaa caatataaga caacatgaag
    21361 aagaattatg tcaatatatg atagacaaat tagaggaagt accagatata aagatatatg
    21421 gaccaaagga cagtaaaaaa agagcatcag taatagctct aaatatagga gatatggatt
    21481 ctggagaggt tactttctta ttagatagtg attataatat agcgactaga tcaggaatac
    21541 attgttcacc actagcacat accactttag gtactttaaa acaaggtgca gtaaggttca
    21601 gtataggata tttcaacaca aaagatgaga tagataaagc agtggaagct ttgaaaaaaa
    21661 tttcaaaaaa taaatagcat gagatttaat aatatagaaa taaaaaaggg gatatttcaa
    21721 aataaaaatt tgaatcatct ccttttttgt acaaaaaaga aaaggggtcc ctaagaacct
    21781 ctaatctatt tgataattgt tttaagaagt tactttaagt aatctccttc ttcaagaact
    21841 atatgaccac tttcgtaatt agaaccatca acagtaattt ttatttgttc aacattcata
    21901 ttttttatgt aagtacgagc aactgaatca agcattaaag tttctgcatc actacctaag
    21961 tttgactttg taaagtcaga gctaacatct aatataccag tttttacacc atcaatgtct
    22021 tttgtagtaa atgaatttaa ttttgcagat tctggaacaa ctttaagttt ttgaagttct
    22081 tcaaataaag tgttctcatc aattttattt aaatcaactt tattttcaat taaatctgtc
    22141 ttatctacat caaaagagta aatagtagct gattgagctt tagtttcttc ttttggagta
    22201 agtttagctt tgttattagg aacctctttt tcagtattgt tttcttcttt tggttgctca
    22261 acatttgtat tgctcttagt ttctttttta gatgaatcac ttccattttg agcattagta
    22321 caaccaactg cgaatataga tatagataat atacttaaca ctaacattat tttcttattt
    22381 ttcataaaaa tttctcctct cttaaaaact attcttatat ataattatag tcgttatgag
    22441 caaatatatg aatacaaaat agtaacaaat ttaaaaaatg atttatttaa attaataaaa
    22501 ctcaaaataa actaaatttt agtcattttt atgggaaatc atttaatgta cagtgaaaat
    22561 attgtaaaat aaaagtagtg tttttaaaat aaatataagt tataattata ttataaaaaa
    22621 atgtcttcaa aaggtgggaa tagtatggaa attttgagct taggagaaaa gattaaaaag
    22681 ttaagaaaag aaaaaaacat gacattgaaa gaattagctg gagataggat aacagcagca
    22741 caaataagcc atatagagag agataaatct catactagtt atgaactgtt ggaatatcta
    22801 ggcgataagc tagatgtaag tgtagaatac ttattagaga ccaaggaaat gcaatcaaaa
    22861 aagataactg ataatttaat actacagagc gaaatttata taaaagaaaa tgaattaaac
    22921 aaggctgagg accaaataaa agaagtttta gatatatgta agagatacga tttaatggag
    22981 aactatggca gatgtaattt tttaatggct aatattaatt tgaaaaaatc taattataat
    23041 gatgctatag aaaactttga aaaggcattg tacttcttta taaaaaatag tgatagtgaa
    23101 aatatactta agtgttattt aaacataggg aaaatatata tgcaggaaga cttttataaa
    23161 ggtgctataa gccactttaa ttttgcagag gaaattttgt cagagaataa atttgaggat
    23221 gttaacatac acaaggaact atatagtaag atggcatatt gttacataaa actagataat
    23281 ccaaatatgt cattgaagta catagaaaag ataaatgaaa tagataatag aaatgataga
    23341 aaagaagatg tcgatatttt ggtgttaaaa gcaaataata tgcttgagat tggaaaatat
    23401 gaagaatcaa gagattactt taaaaaagca ctaaaaatac ttgagagtga ggataataaa
    23461 actggattag caaatgtgta tttaacaatt tgtgatgttt acagaaaaat aggtgaaaca
    23521 gatagagtac ttgaatattc acaaaaggta tatgacataa agaaaaatga tgaagatgaa
    23581 tacatgatgt caggattatt taagataata gaagcatata tagataaaga agattatgat
    23641 ttagctagaa aatattgtaa gatagcctta gcttcatcaa taaaaaataa aaataaattc
    23701 aatgaatata aagcattgag attctatgct aatatgtata aaaatcagaa tgaagtaact
    23761 cttgcaattg aatatctgat taaatgcata aaaatagtat cagatttagg caacaataga
    23821 atattggcca atttatatat agacttaggt caattatatt caaatatatc aaaagaaaaa
    23881 gagttagaat attatcaaaa aggtgtattt atgtacaaaa acttagaaat aatgtagggt
    23941 aaataaacct ataagggtat agaaacaatc tataacaagc aagataatat ctatgataaa
    24001 atataaattg tatgaatatt tttaaggaga tgaatcagac aatgagtata aaaatagatg
    24061 cgagagggct tgcttgtcct aaaccagtta taaatacaaa aagagaattg gataacctag
    24121 aagaaggtgt tgtagttaca atagttgata atgaaacagc taaggagaat atattaaaac
    24181 ttgctaaatc tttaagttgt gaagcaaata ttgttgacga aaaagagaat tttatatcta
    24241 tagaaattag aaaaggtaaa aatatactag ataaacaaaa tgttgtagat aataaagaaa
    24301 gtgaattaga caatacatgt atatttatat cttcagataa gatgggactt ggaaatgacg
    24361 aattaggaaa agtgttaata aaaggattta tatatacatt aacagaatct aaaccttatc
    24421 ctaagtatgt acttttagtt aatggaggag taaaattaag tgctgaaaat gaagaaacca
    24481 tagaaaatct aaaaatactt gagaatatgg gtgtagaagt tttatcttgt ggtacttgtt
    24541 tagattatta taatttaaaa gacaaattac aagtaggtac agttacaaat atgtatacta
    24601 tagttgagac attaaagaat gcatcaaata ccatttctat ataatgtatg tatgtttaat
    24661 aatataaaac taacaataaa gattaactca aatagatagt cagaaaggat taagtaatga
    24721 atcaaatgta tatagtgtca tttaattcga cacatcatgc gataagatca gaaaagttat
    24781 ttggtgaaaa tagtctaaaa gtaatggctc ttccaacacc aagagaaata acagcaagtt
    24841 gtggaatatc aattaaattt tcttttgaag atatggaaaa gataaaaact attttggttg
    24901 aaaataatgt agatataaaa ggaatttatt gtataagtaa attagaaaat ggttcaaaag
    24961 aagtagagaa gttagattag gaggctaatt atttatgcca atggatttaa aaataggtga
    25021 tattgttgaa ttaaaaaagc aacatgcatg tgggtgtaaa gagtttgaaa tcataagaac
    25081 gggaatggat ataaagataa aatgtactaa atgttcaagg ctaataatgc ttgatagaga
    25141 gaccttagaa aagagagtaa aaaagctaat aaaaaagtga taaaaattta acacttaaaa
    25201 aatgtcaaat accgtatatt aatatccaaa attttggtat aataagaatt gaagaaaaca
    25261 ttttcatggg ggtgctttaa ttatggcagt taaatatgca aaaagaatgc aaggattaca
    25321 aggatcagaa atacgtgagc ttttaaaact tactcaacaa ccacaaataa tatcttttgc
    25381 tggtggaatg ccggctccag agttatttcc agttgaggaa atgaaaaaag tatcagttgc
    25441 agtgcttgaa gaaaatggaa gatcagctat gcaatatact acaacagaag gatatgaacc
    25501 attaagagaa aaaatagcag ctagaatgaa tgataaaaac aaaactaatg taaacaaaga
    25561 tgatatatta gttacaagtg gttctcaaca aggtctagat tttgcaggaa aagtattcat
    25621 tgatgagggt gatgtaattt tatgtgaaag tccatcttac ataggagcta taaacgcatt
    25681 taaatcttat caacctaaat tcatagatgt tccaacagat tctgatggaa tgataatgga
    25741 agaattagaa aagatactag aaacaactga tagaataaaa atgatttacg taatacctga
    25801 cttccaaaat ccaacaggaa gaacttggcc acttgaaaga cgtaaaaaat tcatggaaat
    25861 agtaaataaa tttgaaatac cagtaataga agataatcca tatggagatt taagatttga
    25921 aggagagact ttaccatctt taaaatcaat ggatacaaaa ggattagtaa tattcttagg
    25981 aactttctct aaaatattct gcccaggata cagattagga tggacttgtg cttctccaga
    26041 aattttatct aagtttaact ttgcaaaaca aggtgcagac ttacaagcat caactatatc
    26101 tcaaatggaa gtaagcaaat ttatggatat gtatgaccta gatgcacatg ttgacaaaat
    26161 aaaagcagtt tatgttaaac gtagagatgt aatgcttaag actatggaag aagagttccc
    26221 agaaggatta gtatttactc atccagaagg tggattattt acatgggtag agcttccaag
    26281 taatttaaat gctaaagaat taatgccaaa atgtttagat aagaatgttg cttatgttgc
    26341 tggaggagga ttcttcccta atggtggtag agaaaatact ttcagactta actattcaaa
    26401 tatgccagaa gaaaaaataa tagaaggtat aaaaaatata gcagctgtat taaaagaagc
    26461 tatgggtgta gaagcttaat taaaaataat aacttatata taatacaagg gatggttgtt
    26521 gccatccctt gtttgtttga aaagtatata attcacctta tactaaactt tgtccaaaat
    26581 ttaaattgac agaaataaga ataaatgata aaatgtgtag gtatgagatt ttagtctcat
    26641 ttctctgctc taagagtgta gagccgttaa gtccaaaggg aggtgaatta tagtgagaaa
    26701 ttatgaatta gtttatgtgg taaagccaaa ctctgatgaa gaagtaagag aggctatact
    26761 aaacaaagtt aaggaagttg ttgcaactga cggagaaata gttaaagttg atacttgggg
    26821 aactaaaaaa ttagcttatc caatagctaa gtttacagaa ggtttctatg tattagttaa
    26881 cttcaaatca gcagtagacg ttcctaaaga gatagacaga aacttaaaga taaatgaaaa
    26941 cgtaataaga cacatgatag ttgttgctta ataactggtt ttattataaa aaataggtgg
    27001 tgtttttatg aatcaagttg tattagtggg aaggttgact agagaccctg aattaagata
    27061 catcccaggt acaggaacac cagtggcatc ctttacaata gccattgata gagactatgt
    27121 aaaaaaagat ggatctaaag aaactgattt tatacctgtt gaagtaatgg gtaaatctgc
    27181 tgaattttgt gctaactaca taactaaagg tagattagta gcacttcaag gatctataag
    27241 agtagacaat tatcagacac aatcaggtga aaagagaact tttacaaaag taagtactag
    27301 atcagtacaa gctttggata gtaagaataa atcggaaaat tcatatagag agagtgctcc
    27361 agcttttgag ccaagctttg aacctcaagg tttagaccca caaggtttcc aagctataga
    27421 tgatgacgat ataccatttt aaaaaggagg gaaaaaagtc atgatgaata aaaaaagacg
    27481 taagaaaaaa agagtttgtc aattttgtgc tgacaaaaac gctaaaatag attacaaaag
    27541 tactcaaaga ttacaaaagt atataactga aagaggaaag atattaccaa gaagaatatc
    27601 tggaacttgt gctaagcatc aaagagaatt aacagttgct ataaaaagag cgagaaatat
    27661 agcactttta ccatatacat tagactaaaa aaatgagcct tgtgctcatt ttttattata
    27721 tgtatttata tatctaaatg aggcgagcta taaagctcgt ttttttgttt atatacttaa
    27781 atatattatt atttatgata taatattata tattaaggag ggttgtaaac gtgaaaatag
    27841 ataaaggttc tgagataacg agaaacataa agataataga atggatgaaa acagaaatat
    27901 taatgagtgt aagtgatttg tttaatttat tgtttaaagg ggtaaaacca ttagatgaag
    27961 caattcaaga tactttagca aatataataa tgataacata tctcttagca aagagattag
    28021 gaattagttt tagagatgtg gattataaag tcaaggaaaa aataaaaatt ggtatagatg
    28081 aggaccatag cgttgaaaga tggtatggag atttatctaa tttaaaaaaa catatggata
    28141 atagggagtg atattaaatt gaataataaa attagactat ctaaggcaat gtctataata
    28201 gttttagcta taataatcgc tttagtaata gcctatattc caatgctagg aatatttagt
    28261 atgttagcag ctgttccata tgtaattata ggtgctatta ctgatagaaa gtattctctt
    28321 atatctattt tggttacatt ttgtgtactg attctatttg cagacatttc ttatgcatta
    28381 aatatatgta taatgtattc tttaccaggt atagtaatag gaaagatgct aaagagaagc
    28441 tttgaacaag aggacagtaa taaattcgaa ccgatatatg gtggaaccat aatatttgta
    28501 ctttcaatgc ttgtatattt ttttgtatta aagacatttt taaatgtgaa tctattagat
    28561 gaagtttcta agatgatatc agaaattgta aatattcaaa aaaatagttt ttctgcaact
    28621 gaacttaaag tgtttgataa aatgaagcca gatgagattg taagttattt tacaaatatg
    28681 ctaccaatga tgttattttt acaagggttg ttatcagcgt ttgtcacata ttatttgagt
    28741 gtatttttta taaaaaggat tacaaagatg aatataagat ttcctaagtt tgctgatttt
    28801 tatttaccgg gaaatgctat actcacaact ttcttacttt atttattagt cttatttatt
    28861 caaattattg gttcaaaatt atatacagaa ttaataatga ctaatttaca gctagttttt
    28921 aatttaatgt ttgtaattca aggtatagca gtttgcattt atttcttaaa gaaatggata
    28981 agacagggac caaataaaat tatgtttttt tcagggataa ttttatgttt atttggattt
    29041 atgggaatat cttttgttgg tatggtagat agtataattg actttagaaa ggtaagaagc
    29101 tataaatcca cttaggagga tagaaatgag taataaacaa acctttaaat taaatatgcc
    29161 agaaataaat ttatatataa ttgttattgg tatttctagt ataattttgc tttattacaa
    29221 cttgtatgta gggtgtttat ttttctgtat ttttgtatat atggtttttc acaattggag
    29281 aactactaat attagacgtc atgaatggac agagtatatt caaaatttat ctttagatat
    29341 agatgaaaca actaaaaaag ccataataaa tcttccaata ccattatgta ttttagaatt
    29401 tgatggaaat atatcctggt ataatggaaa gttttatgat atgattggtc aaaaagattt
    29461 acttgataaa aatatagagg atatagttaa aaatcttaat ttaagaaaag tattaaatga
    29521 aaataaagag atgtacacag aaataaacta taaagaaaaa gaatatacaa taatttataa
    29581 tgttataaaa aatgatcaag aaaaaaatcc taaatatttg atgatattat actggataga
    29641 caagactgaa tatttaaaag taaaacaaaa ttatgatgat gaaaaaaatg caatgatgct
    29701 aatacaagta gatggatatg atgaagtatt aaaaagtgca gcagaagata agagagcttt
    29761 aattaatgtt gaagtagaga aaatactttc tgctttagaa ttaaattcaa atggtgcatt
    29821 gagaagaact tcaaaagaca aattcttctt ggtaatgcat aaaaaagagt taaaaaaatt
    29881 ggaagctgag aagttttcta ttttagatac aataagacat attgattatg gaaataatct
    29941 tcctgttact ataagtatag gaattggtat agatggagat acattaaatg agaacctaaa
    30001 acttgctacg ggtgctctcg atttagcact tggtagaggt ggtgaccagg ctgttgtaaa
    30061 aacaaaagat aaatttgtgt tttatggagg aaaatctaaa gctgtagaaa agaaaaccaa
    30121 agtaaaatcc agattaatag gtcatgcttt aagagaggtt atacaacaaa gtgaccaagt
    30181 ttatataatg ggacataaat atcctgatat ggatgctatg ggagcagcag ttggtgttta
    30241 tgatatatgt aaatcctgta acaaaactgc aaacatagtt ttacaatctg taaatgaatc
    30301 tatagaaata tttataaata aaataaatga aaataattac tataaaaagt tgtttattgg
    30361 aaaagaggaa gctattgaca actgcactaa aaatacatta gttgtagtag ttgatactca
    30421 tagaccaaat tatactgaat gtgaagaact gttaaagcta tcagagaagg tagtagttat
    30481 agaccatcat agaagaggtg tagagtttat aaatgatgca gtgcttttat tccatgagat
    30541 atatgtatcc tcaacgtgtg aaatggttac agagttagtt cagtatatgg atgaagatgt
    30601 aacaataaat aaacttacag ctgaagggct tttagcaggt ataagtctag atactaaaaa
    30661 ctttgcattc aaaacaggtg ttagaacttt tgaagctgct tcttacctta gaaaagttgg
    30721 agcagatact atagaggtaa agaaattctt caattctgat gtaaaagatt tcataataaa
    30781 agctgaaata attcaaagta ctaaaataat taataataga atatgtttag catattcaag
    30841 tactgagata gatagtataa atgttataat agctcaaact gctgatgagt tattgaatat
    30901 caaagaagta gaagcttcct ttgtattagg tgaaaaagat gatactatat ttataagtgc
    30961 gaggtcttta ggacaaataa atgtacatgt acttatggaa aaattaggag gcgggggaca
    31021 catagatata gctggagcac agttaaaaaa tgtttctcta aaagaagctt ataagatggt
    31081 aaataaaata atagaggagt atttggagga ggaagaatag atgaaagtta tattattaaa
    31141 agacgtaaaa ggaactggta aaaagggaga aatgaaagaa gtaagtgatg gatatgcaag
    31201 aaatttctta tttcctaaaa agatggctgt gcaagcagat agtgtagcaa taaaagaatt
    31261 aaatgaaaag aataaatcta aagaaataaa agcacaaaaa gagtatgaag aagcagtttt
    31321 attaggtaag caaatggaag aaattaatat agaaatatat tcaaaagcag gagaaggtgg
    31381 aagattattt ggttcaataa cttctaaaga aattgctgag caattaaaga aacaaaaaga
    31441 catagatgta gataaaagaa aaatattatt agatgaaccg ataagatctt taggttcaac
    31501 tttcgtagaa ataaaaatac atcaaaaagt aactactaaa ataagagtgg atgtaaaaga
    31561 aaaacagtaa taactagagg tgattaagat ggaagatatg acgagaattc ctcctcatag
    31621 tgtagaatca gaacaatcaa tattgggttc tatacttcta gataaagatg ctataataac
    31681 ggttacagaa actataaagc ctgatgattt ctataaagag gctcacaaaa taatatatga
    31741 atgtatgata actttaagta ataaaggtga gcctatagac ttgataacat taacagaaga
    31801 actaagaaaa caagggcatc ttaatgatat tggaggaatc agttatatta caagtctttc
    31861 tactatagta ccgacgactt caaatgtaaa atactatgct gatatagtaa aagaaaagtc
    31921 tgtacttaga aagcttataa aagcttctaa tgaaataata aatctaggat atagtggagc
    31981 aactaagatt gaagatgttt tagaacaagc tgaaaagagt atatttgata tatcacagga
    32041 gaaaactagt gatgatttta aatctataaa cttagttttg atggatgcct atgatatgat
    32101 agaaaaatta tacactaaca agagtgatgt cactggtata actactggtt ttaaagattt
    32161 aaacaaaaaa ataaatggtc ttcaaaggac tgatttaata ttaattgcag ctagaccagc
    32221 aatgggtaaa actgcctttt ctttaaactt ggttcaaaat gcagccttaa aaggtgatgc
    32281 ctctgtagct gtatttagtt tagagatgtc taaggaacaa ttagtacaac gtatgttatc
    32341 ttcacaatct agtgtagaat taaaaaaaat aaagactgga acacttaatg ataatgattg
    32401 gcctagaatt atagatgcta tggcagtatt atcagatgcc aaaatacaca tagatgatac
    32461 tcctggtata aaaatatcag aactaaggtc aaagtgtaga aagttaaaaa tagaaaaagg
    32521 actagattta gtgcttattg attatcttca acttatggaa ggtgaaggta ataatgaaag
    32581 cagacaacaa gagatatcta agatatcaag gtcactaaaa atattagcaa aagaattaaa
    32641 ctgtcctgta gtagcactat ctcagttatc acgtgcacca gaacaaagag cagaccatag
    32701 acctatgtta tctgatttaa gagagtctgg agcaatagaa caggatgcag atatagttat
    32761 gttcttatac agggacgaat attatcatgc tgattctgag agtaaaaata taggagaagt
    32821 aataatagca aaaaatagac acggtgaaac tggttctgtg gaacttgttt ggcttggtga
    32881 agttcaaaga tttggggata agttgagaga cctataatct aacgaatatc cacagaacgt
    32941 cctttctttc tacaggaaaa atgtggatac atgtcactaa atatgtccaa acattgaaat
    33001 ttagctaaaa aactatccac actatccaca gatggcctgt tgataagtgt gaataagtag
    33061 ataaagcaag cttttggctt gctttatctg tgtataaata ttaattatta ggaggaataa
    33121 tatggaaaaa ataagcttaa aatatatata tcccaatata attaaagtac ttgatgaaat
    33181 aaacttattt agagtaattg acaataattt aagagagtct attgttgttt atgctaataa
    33241 tgtggataat cagtatcata taaacatgac taatacaaac tttggaaaca taattaacat
    33301 atgtaaatta gaaaaacttt tagatgtgga taaatttatg gaaaaagtta taaaatatga
    33361 aaaagaaata atagagaaag aagagttttc aaaaatagaa gaatatatgt taaatattgg
    33421 agaatactaa aaaagttgaa aaaaatacga atgtttgata gaataagtaa gtaaagtgtt
    33481 cgagagtata taaaatatag aggtgaatta catgaaaaca gttgcaattg ttggttctca
    33541 atggggagat gaaggtaaag gtaaagttat agattatctt gctactcaag cagatgtagt
    33601 agtaagaggg caaggtggaa ataatgcagg acatacatta gttgtagaag gtaaaaaata
    33661 cgcattacat ctaattccat ctggagtttt aaatccaaat acagtgaata taatcggaaa
    33721 tggaatagtg tttgacccta aaggattttt agaagaattg gaaatgttta aaacggataa
    33781 tataagtaca gaaaatataa aaataagtga tagagcacat gtcatattcc catatcataa
    33841 agaactagat gcattgtcag aagaagccag aggcgattta aagataggta caacaaaaaa
    33901 aggtattgga ccttgttata tggataaaac agagagatct ggtattagaa tatgtgactt
    33961 aatggataaa gataaatttg ctataaaatt aaaagctcaa atagatgcta aaaatgaaat
    34021 agttaaaaat atatatggaa aagaagaatt atttgatttt gaaactatat ataacgaata
    34081 tctaggatat gcagaacaaa taagaaaata tgtggcagat acgtctgtta ttgtatatga
    34141 tgcagtaaga gcaggtaaaa aagtattgtt tgaaggagca caagggactt tattagattt
    34201 agatttagga acttatccat ttgttacatc ttctcatcca acatctggtg gatttgcaat
    34261 tggtgcaggt ataggaccaa atatgataaa agacgttgtt ggtatagtaa aagcatatac
    34321 tacaagagtt ggagaaggac cgtttgttac agagcaaata aatgaaactg gagataagat
    34381 aagagaacaa ggacatgaat ttggggtaac tactggtaga ccaagaagat gtgggtggtt
    34441 tgatgcagtt atagttaagt atgcagctag agttaatgga ttgactagta tttcatttat
    34501 gttacttgat gtattaactg gatttgataa gataaaagtt tgtacgtctt ataaaatggg
    34561 cgataagata ataacggatt tcccagcttc attagatgat ttagcaaaat gtgaacctgt
    34621 atatgaagaa ttagatggat ggaatgaaga tataactcaa atagataact ttgatgacct
    34681 tccagaaaat gcaaagaaat atgtagcaaa aatagaagaa ctagttggag taagtgtaga
    34741 tatggtttct gttggtccaa atagagctca aactataata agaagaaata tatttgctta
    34801 atattttatc aattaaataa tttaaaatag ttgaagaaga gtatctcaag ataatgttta
    34861 tagttattag agatgctctt ttatatttta cttacattga ttttttatta agttaaagct
    34921 acttatacat attctagctg aaattaaagt tgaatttatg tagatacata taggtttttt
    34981 aaaaagaatt ttgttgaaaa taaatggcta aagatgattt taattatatt ctaaaaaaag
    35041 taaaaaaaac tattgactaa aaattatata gacgctataa tataacttgt gagtgaaaga
    35101 aacgacgaaa aaagtcgaaa aaatgtggtc tattagctca gtcggtagag cacctgactt
    35161 ttaatcaggg tgtcccgcgt tcgagtcgcg gatagatcac caaaatatgg ctcgttggtc
    35221 aagaggttaa gacaccgccc tttcacggcg gtaacagggg ttcaattccc ctacgagtca
    35281 ccagtatggg actatagctc agctgggaga gcacctgcct tacaagcagg gggtcacagg
    35341 ttcgagccct gttagtccca ccaaattttg aattagagat aaatactaga ttatatgctg
    35401 gtgtggctca acggtagagc agctgacttg taatcagcag gttgtaggtt cgattcctat
    35461 caccagctcc acaaagtaca taaattcaaa ttaatataat tttctatgtt acaggatact
    35521 tttaagtatc tttttttaat ttcattttta ttgtattaaa aatgaaatta aacatacaac
    35581 aaaattttga attacttgca atgtgatagt tttaaatata aaatttagaa tcattctagc
    35641 atagtattat gtaatatagc aaatttaatt tatagtatca ataaatatag actactaaga
    35701 atgtaaatga tttgttagat taagatacat tagatatgta aatagaaaaa taaaaagtat
    35761 gttttaattt atgtgatata agttttaaac agagaagcaa gtttgctctt acctctctat
    35821 ttaaaaataa aaaaatgttt tttaagggct attttattta aatctttccc atctcaaatc
    35881 atctccagta aattctaaaa tcctaaactt atctaatatt ctagagagag ttctttgagt
    35941 gtaagtattt cctatttgag atggtgttaa gttggttgaa ataatgattt ttttaccagc
    36001 aacaagtctt gtatttacaa tattgaagat ttctccacta gtaaaagaat tattaagttc
    36061 agttccaagg tcatctatta ttaataaatc acaatcaaac aagtttttat aattatcttc
    36121 acttatttga ttatttgtgt ctcttctaaa tttataatct tcaagaatat ctaaaattct
    36181 aaaagaagtt tgatagatta caacattgcc tttatctaac aattcctttg caatacaatt
    36241 gcacatatag gtctttccta gtccagtaga accataaaac agtagatttt cactattatc
    36301 cttcttaaaa tcatgaacaa aattttcgca gatacttaaa ttatttagca tattttctct
    36361 aggagatatt tcaccatcag agcctttctt aggagaaaat atattcaagt taaaattcga
    36421 aaaattttcc tgacttaaaa tcctatctaa attagacatt ttataagctt catttacaat
    36481 ttcttgtttg aggcaagaac atttttctcc attaggtaaa aatcctttat ctttacaaga
    36541 aatacattga aactttattt ccaagtaatc aagaggtatg ttactttcag ctaaaagtct
    36601 ttctttcttt acttttaaag actctatatt ctctttagtc ttctttacaa tttcatcttt
    36661 agacttagga tttaaaagta ctatcttagc taaactgaga cctattttag aaatttcatc
    36721 atctatagat tttatctcag ggattctatt atatacctca ttttttctat gttctaataa
    36781 gagttcatta tcatctcttc tcttagcata tttagcaagt atttttctta ttttatcctc
    36841 attcatttaa tcacctactt aaacttttct ttttgacttt ttttaataat ctcatcgagc
    36901 tcatcagagg tatactgagt gaatgtttcg ttgaaattat gaaacttagt ttttttgtaa
    36961 gtattgttgt tttgagtagt attaatctgc ttcttattaa tattctcctt aactatacgt
    37021 tcttcctctt tttgctttaa atcatttaaa ttttttatat ttttttcatt ccaattttta
    37081 attataccat ttatataaga tatacttggg tttgaaatat tcttagactt agaacatgca
    37141 tttattatca aatctatatc catattaaat ttatcaaacc aagtgtccat aagttctttt
    37201 tctgattttg atggttgtct tgaaaatccc aattcattga aaatcgtttt atataaaata
    37261 tatcgttcac ttcttaccaa aaagctttct tcaacatctt ttggcgtata taaatttgaa
    37321 tcataccaat ttctaataat gccttcaatg tatttaactg gctttgatgt accagtttta
    37381 tctttgacgt attcatatgc gcaaagtatc atatcggggc tcatattgta tttattcatt
    37441 atatccataa tactcatctt ttcgcttggg tcgagatatc gcccaactat tttatttata
    37501 gaattaaaca tttttctaat acttggattt tcagaagtag atacaacctt atctgtatta
    37561 gattttatag atgaattatt gcttaatata ttctcaatat agaaattttt taaatctaaa
    37621 aattctattg aataattaaa attatcatat tctccattgt catgtatttt tataatcttc
    37681 ttttcttccc agaatttcca agcagataac acatcagaca atggtatatt taggtttttg
    37741 gctattgaat tattgtcgaa cttaggatta gaagtaatat cacatgcttg tctatatcct
    37801 aaaagataaa cttttacgta taacccgtcc gccattggca taaatatatc tataaagata
    37861 ttgggaatag tgatttcacc taaatctatt tcattgcttt ctttaaaaaa cattttatca
    37921 cctcagatta aattatatca ttttttatat tatatttgtc tgtaaacaaa taaaaggtca
    37981 caagattagc atgtaaatat tttctaaaag tattcatttt gaaaaggagg agatattttg
    38041 gttataaaat tcaaccaaaa agtaagaaga acattatcag tagcgattct atttatggta
    38101 ttaataatag gttgctttat agaaaaggat gacagtttag agacatacaa agaaaaaact
    38161 aattcaaaaa taaatcagta taatatggat gttatatttg atgatgagac taagaggtta
    38221 atgtgcaatc agaatgttga ctacataaat aatacaaaat caaacttaga taaaatatat
    38281 ttccatatat atccgaatgc tttttccaag aaagattttg ctccttttga aaaaagtgaa
    38341 atgacaaggg cttatcctaa tggatttaac gaaggatata tagatataaa gaatatatta
    38401 aataataata gtaaaatgga gtataaaatt aaaggagaca aaaatgacat attagaaata
    38461 gaattaggga aagagttaaa accaaatgaa agaatgtcat tagatataaa atataacgta
    38521 aaaataccaa attcaattgg aagatttgga tatggagaaa atactataaa tgttactaat
    38581 tggtttccta tagcttgtgt aaatgatgaa aggggatgga atttaaagag ttatgaaact
    38641 attggcgacc cattttatag tgagactagc gattttcatg tgaaactatt gattccaaat
    38701 aaatataaaa ttgctcatac aggaaaactt attgatgata aacatgataa taagaaaatg
    38761 ctatatgaaa tagaagcgga aaacgttaga gattttgcat ttatactaag tagcaaattt
    38821 aatgtaaaca aagtagattc aaagggagta gctatcaata catataattt aaataaaaag
    38881 ctttctaaaa aagcaactga agtggcaaaa gattcaataa atatatttag tgaattattt
    38941 ggaaagtatc cttacaaaga attttctgtt gtagcaagtg acttttttat aggggggatg
    39001 gaatacccta tgcttgttat gatagaccaa agtttatata atgaaaaaaa tgaattttta
    39061 ctagaatatg ttattgccca tgagacagca catcaatggt ggtattctgc tgttggaaat
    39121 gatgaaataa gtgaaccctg gttagatgaa gcacttactg aatattctac aatagtttat
    39181 tttgaacaaa agtatggtaa agatatggca aacaagctta taaaaactat ggagatacag
    39241 acaaaaagtt atttgagcga aaatatattt aagccagcaa atgagtataa aaattctact
    39301 gaatacagct tgaatgttta tacaaaaggc gcaatagcat ttaatgaaat aagaaaagaa
    39361 gttggagata aagtattctt tgagacttta aatgagtact ataataaata taagcataaa
    39421 aatgctaatg gaagagcctt tgtggaacta tggaatagta aaggtgtaga tataaataag
    39481 attataagtg aatgtaagta aacagtgaat gtaagtaaat tttgtgtaga gtattaaatt
    39541 ttatttaata ctctgttttt ttggtataat ataatttata aggcagaaaa agaggtgaag
    39601 gaatgctaaa gtattgttca gtagggagtg gtagtagtgg taattgccac tatatagggt
    39661 ataaaaatac taatatacta gtagatgcag gattaagtgg caagagaata actacaggtc
    39721 ttaaagacat tggtgtagat gcagataaat taaaaggaat atttataaca catgagcatg
    39781 ttgaccatat aaaaggagca ggtatattat caagaaagtt tgatatacca gtatttgcta
    39841 atataaaaac atggtgttct atgaaggaca aattaggtga catcaaagat aagaatatga
    39901 aagtatttga aaatgataaa acctactcat taggtgatat catagtaaga cctttttcaa
    39961 tagaacatga tgcgtctgac ccagttggat ataattttta tacagagagt gatgaaaaaa
    40021 tgtctatagc tactgatata ggatgtataa ctcaaaatat aaaaaaacat ctatataaat
    40081 ctaaattagt agtattggaa tcaaattatg accctaatat gcttatgatg ggttcatata
    40141 catatgcttt aaagaaaaga gtaatgtcaa gtactggtca tttatccaat gaagatgcag
    40201 ctaatttctg cgttgaactt attaatgaag gtacagaatc tatattatta gcacatttga
    40261 gtaaagaaaa taattttcct gagttagcgt atgagacttc aaaaggagta ttagcatcaa
    40321 atgatatagt agttggacaa gatgtaaaac tagatgtact atcacgaaat gatgtttcta
    40381 atgtttatga gatgaagtag aggtgaataa ctttgctaaa gagaaatttt gaaaatgttg
    40441 aggataataa aattttatac tctataaagc agggtatgat tttagcgata cctgctatta
    40501 tgactggttc aacggctctt gtaattttga atcttccaat aaaggcttat caagaatatt
    40561 tatcaggctt gtttaatgga gaggttacaa atattcttaa ctttataaat gattctactt
    40621 taggaattat ttcattaata atattattaa caataagcta tagttatgga aaaatatatg
    40681 ggagcaagta tacagtactt gttcctatag ttgcaatgtg ctctttttta gtattttcac
    40741 atggtaatga gcttaattct tatatagaaa tatttaaaac aaagtggtta tttacatcta
    40801 taattgtttc tatgacatct tctgtattgt ttgtaaaact tacagaatct tttgttacaa
    40861 gtgtaaagtt tcatacagaa ggggctgatg cagactttaa tatggtagtt tcggctatag
    40921 ttcctttcat aatagtagta tttttgtttt ctatttttag agtgataatg ataagtctaa
    40981 ttggcagttc aaactttcaa gatatatttt ataatttatt ttcaaatgtt tttaataaaa
    41041 tgggaacaaa ccttatgagt gctctgttgt ttattttttt aatgcacttt atgtggtttt
    41101 ttgggattca tggtagtaat gtattagata ccgtcgcaaa aaatttattt gaaaatagta
    41161 tggcaattaa cataaattta gttaataata atcaattacc aacagaaatt tttactaaga
    41221 ccttttttga tacaatggtt ttattgggag gatgtggctc tttactatgt ttagtaattg
    41281 caatttttct tagtgaaaaa cgtataaatg taagaaagtt agctaaaatt gcttcaatac
    41341 cagctctttt taatatcaat gagatgctag ttttcgggat accaattgtt tttaactata
    41401 ttatgtttgt accttttgta attactccta taatcttaac catcacaagt tatgtggcta
    41461 tgtctacagg tattgtacca tgtacagttt cagctgtaga atggacctca cctatatttt
    41521 taagtggata tatgtctaca ggttcaataa gaggtagttt gttgcaagta tttaatttat
    41581 gtgttggtgt tatgatttat attccgttta taaaaatgtc gcaaaataga tatacatata
    41641 tgtttaaaaa taatatagaa aatttaacaa acttattaaa aaggtatgaa ttaacaggtg
    41701 aacagcctaa tcttctaaac cataatggta aaataggttc aatttcaaaa atgttggcat
    41761 cagacttgaa gtttgctatg aaaagaggag aaatagagct tttttatcaa ccacaagtca
    41821 attataatgg aaatgttgtg ggagcagagg gattacttag atggaaacat tcaataggag
    41881 ggttcattta tccaccacta gttataattt tggctaaaga agaagggttc ttagatgagc
    41941 ttggagagta tattattaat aaagcatgta ttgatttaaa gaagagtgaa aagctattga
    42001 gaaatcctgt taagttctct gtaaatatat ctccagacca acttgatgac cctaagttac
    42061 cagagcaaat aaaaaatatc attagtagta atgatataaa tcctaatatg ttaggtatag
    42121 aaataacaga gcaaattgca ttatcaggtt cacaaataat tattgaacgt atcaatgcga
    42181 tacataactt gggaataaag ttaattatgg atgattttgg gatggggcat agctctctta
    42241 tatatcttca aaataataac tttgatatag ttaaattgga tggttcatta gttaaagaga
    42301 tacttacaaa taaaagaagt agtgaaatta taatgactat tgtaaatctt tcaaaaaatt
    42361 tagattttga tatagttgtt gagtatgttg aaaatataga ccaaagggat aagttatatg
    42421 gtttaggatg tgaaatatat caaggatatt attatagcaa agcaatacct tttgaagagt
    42481 ttgtagaata tgcaaatcaa gaaagagaga tataatttta taagagcttg taaattctag
    42541 taatgcatag atttgtaata aaattaagtt taggagaaat atatgaatat aagtatagta
    42601 gtagtaggta aaattaaaga aaaatattta aaattaggta ttgatgagtt caagaaaagg
    42661 ctttctaagt attgtaagtt agaaattata gagttggatg atgaaaaagc tccagagaat
    42721 ttgagtgtta aggaaatgga aattataaaa gacaaagaag gaaagaaaat tttagggaag
    42781 ataaagcata atagttatgt tatagccttg gctattgatg gtaagggttt atcctctgaa
    42841 gaacttgcta agactatgag tgatttagca gttagaggta atagtagtct ctgttttata
    42901 ataggaggtt ctctagggct ttctgatgag gttttaggaa gggctgatta taggttatct
    42961 ttttctagga tgacttttcc tcatcagatg atgagattga tattgttaga acagatttat
    43021 agggcttata ggataaataa tggagaaccg tatcataaat aaatgatacg gttttttatg
    43081 atataattga attataaaat aaaatcaaaa tgtaaagtag gtgtagtaat ggacagacta
    43141 gatatattta tcgaaaatta tgattttaaa gaatatcttg aaaaattacc tagagaatta
    43201 atagaagaaa attataaaga ttttgttata gaattttgga aaaatcctag tgtagatatt
    43261 attaaactaa aatcaaaata tagtataaaa aatgtagtat ctataagtag tatattaatg
    43321 caaaatccta tccaagagta ttatgagttt cttgaagata tttggaaaaa taacataaaa
    43381 atagatgaat taattaataa gtatttaatt acagcaacaa gaatggatca aattaaattc
    43441 ccattaatat atattgatga agattctttt tgtccttatt gtttaggtaa taaattcaat
    43501 attattaata atattaagca acaagatata aaagttgaat gtgaaaagtg tggtgaggca
    43561 attagttatt ttattcataa ggaagaagta gataatataa tagaagaaga aaaaagaaaa
    43621 aaagaactat ttataagaac attggagaat gtatataata aaataaacaa gattaaatgt
    43681 catagatgcc aatcagattt atatgtgtgt attaatgata aaataatgga gtataatatt
    43741 aggtgtgata gatgtaattc acagtatgaa tcggttgaaa ttgcacagga acaatataga
    43801 ttatggcaac aaagagctgc aatgatggct aacataaaaa ataaagaaaa tgaattgttg
    43861 ctaaagacac tatcaattaa ggacgaaaaa gatattaaat ttaaactcga agatataatt
    43921 acggagagtg aaagtataga tgtaattagt gatttagtag atatgcaaga tagaggagag
    43981 aatattttca agcaattatt taataattat gttagactag gaagattaga cagaaggtta
    44041 ttggtaactg tgtgtaaaga ttgcaagcaa aatgaaaata cttatacatg ggaaagaggc
    44101 aaagaacatt taataaatat gagggggatt acatacagtg aagcagttgt atatgagtta
    44161 atggaaaaaa caaagctatt gccaataaga gcaattatta gaagacttat ggagaaaaaa
    44221 ctattgtatt gtgatgaaag gagcaataat ttacaagtac accctgtttt gattgaaaat
    44281 ttagctaaac tagaatcaat aaataaatct caaaatataa ataatcaagt agcttatcta
    44341 atattcaata agcaaaaata tatatgttat cattgtggtg aatcaggaag acccttaaag
    44401 ataggatatc taacaataga taaaaatata aatgatttat caagtttagt gggaatatgt
    44461 gatagttgct atgaagatat tactgaagat gaaattatta tagataatat tttttgtgtg
    44521 gtagatgaaa gaaacaatga attaggggaa gcttggaaat ttttaataca atatgatagt
    44581 agttttagaa ataatacaat tgcagaagta gagcatgcaa gattatatga tatgtatgat
    44641 gaagataatt taattcgtgc gtatgcaaat acattgtata aacaaacgaa agatgggttt
    44701 gctaatgaaa aagtattttt tgcatatgca agagcaatat tgaaaaatag caaagggcaa
    44761 gtaagtgtac tacaaatagc aaaagaaaaa tttaaattag aaaagtggtt aaatggataa
    44821 agggataata catattaaaa ttataaattt atataaaact gtattgcaat taagcaatac
    44881 ggtttttata tataagataa tgatacaatt aaccaaaatg tggtagtaca taagtgttat
    44941 gagatttaaa tattagcgat taaaataatg attaagtaaa attgtaattg atgaggagta
    45001 ttaacgtatt agtttatttt tatatataat taaaatatag gagggaagaa tgtgagtcat
    45061 agtaaactat ctgatcacaa attaaaaaag gggaaattta tagcaccttt taatgaattt
    45121 atgacaccat tgaatagtaa tgagtcatgg tgccttggtc gattgccaga gtatatatgg
    45181 ataggattat taataaataa atatggtaga aaaaaaggga tggaaataat ttataaaata
    45241 attgtaaaac ttcattcaat tgaatcagat attgtaacac ctaggttgtc tgagatttta
    45301 tctatggagg atgtaaagca ggatgaattc tttagttata ttctaactat tgttgaaaag
    45361 gaagctttat ctccattgac attaatattt acatatagta attatcctat ttttgttaaa
    45421 tattttgcag agccaagtat aaaaattgaa gatagggtta cctatttaga tgagacttta
    45481 agaaaagcta tgcatcatca aagtgaattt gctacagaca taagatttat agttttatat
    45541 tatttttgta tgtcaggaaa actcgtattg caaaaagaac agattaataa tttgctggaa
    45601 tatcctaatt tagaacacac tgatgaaaaa atgagaatgt tacgtccgtt tattcgtgca
    45661 gcagaattaa tggttttaaa tatggaaaaa aaatctgatt ggtatttaga tattttttgg
    45721 gggaggatta gtgttatgac tgaatgtaaa ttatacgcaa ctaaatttga agatgaaact
    45781 gctaatacag acttgtatat ggaacaatta catgagattt ttgtttattt atcagattta
    45841 ctatgtaaag taaatccatt agacgagaaa atgttagtat tacttggaat agcaatatat
    45901 tcttataaac gattacgaga agtagttgag cataaattat ttaattccat ttcaggtaga
    45961 ggctcagtaa gggtattgat agaaaattat attatgatga agtatttagt aaaaatagaa
    46021 gctcaacatg aaaatatatg gtcagaatat caattttatg gaataggtca atataaatta
    46081 attgtggcta gatatagaga acaagaacaa gatttaaaaa atagtcatgt tgattataaa
    46141 tatattgaag ctttagttaa tgaatttgta attgaagaaa caatagatat ggatactaaa
    46201 tattttgata agcaaaatat acgtagtaag gctgaaatag ttggagaaaa agagttatat
    46261 gggttgtact atgattatga ttccgcatat gaacatggtt tatggggatc aataagagaa
    46321 agttcaatgt taaagtgtga taatccagct catcactatc attgtatacc agatgtagac
    46381 aataatcaaa atttaaaaag tgtttggcat gattgtgtaa acataatgga aaaaacttta
    46441 cttttcttag atactgtata tggtatacca caacatttgc tattagaggt gataaaaaat
    46501 ggaaaagaat tttttactaa ataaaataaa tagtattcag aaggaatatt tagaattatt
    46561 agtaaagtta aaagataata tagatactat agattatatt tatataatag atgaaataaa
    46621 tatattttgg tattcatata gagatgttat aaagctttac ttagggaata ttacacaaaa
    46681 agacaatgca tatattttta caggtgcaac atatctagat gttgatgatt tagaacatta
    46741 tccatttgtt atgatgggag atatacatat agtagatgat tcgctatgta aatattcaaa
    46801 aacagtacta gtattatcaa atcaggaatt tgctaaagta ttaaaagacc aaattatttc
    46861 ttgtatagat gataatatca aaatattgtc taattattct tttgcgatgt taatattacc
    46921 aatgaattta ctatatggta atgacatgaa aataatacat gaatattcaa ataatatatt
    46981 tctgaacttt ttttcaagaa aagacttaaa cttagatact tactttgaag aatttaactc
    47041 aatcgaagat atagctctag cattgaatcc aggtatagag agttatattg tttttgacga
    47101 aggtgaagat ttaactaaaa atctggaaga aagatttaaa agctttattg ataaggggaa
    47161 ggtaccccta ccagcacata aaagtgatgc ttttaaattt tggctgataa tttatggatt
    47221 tataactcag gcattagata ttatattgtc atgtaaagga tttaacctaa ctccttattt
    47281 aagatataga gttacattac aatatgtttt attactaggc gataatgttt ttgatattga
    47341 agatctcagc aatatgttgt taaaatgtaa agtagctcat gtactttatg caatatttga
    47401 taaaagcgaa ataaaaaata tttcctttgt tcaattttat aagaaattaa gtgaatataa
    47461 atttagctat aaagtagaaa atgatatcaa agaaaatata aatataaatc aaattgctga
    47521 aattttaaat aaaaatatta cattgttttt aaattatata taaatttatt tatataatta
    47581 cattccttag ggtacataac agaaaaaagg ctgtcgcact aaaaaatagt gctacgccct
    47641 tttttataca aaaaaataaa gctcaagttc aaaagaacct gggattcctg tataataatt
    47701 gtatgctaaa acaaaatact atacaaattg attatactac ttatggtaat aattatcaat
    47761 ttaaattacc actaaatatt gattatatga ttccaaaaga tgctatattt gttgatggta
    47821 caaaaataga gtcatctgcc aatagatata cttttgtttg gaaaaaaact ataaacaaaa
    47881 atatggttag agtgatgaat gatatatcta atttcatatt aaaatgtggg attgattttg
    47941 gtattaaaat aatttataga aataaaatca aaatatatca tttaaaaaaa cttttaaaga
    48001 agcttaaaaa aatatcaaaa gaatcaaata taacatttgt tcatggaaaa ggaaagaaaa
    48061 aatctattgt tcaaaaatct atagcgaaat taaataatta tatcgataga ttgaaaaaat
    48121 atactaaaga ccttcatata atgggtgaac taaatagtta ttctaaaaca gataatgacg
    48181 caactttcat gagaatgaag aaagaccata tgaaaaatgg gcaattgaaa ccagcatata
    48241 atatacagtt tggcgtagat tcagaataca tagtttgggt aagttcagag cataaaccta
    48301 ctgatactac aacatcaata ccatttttaa aatctattga agagcataca aaatttaaat
    48361 atcctaaaat agtagctgat tctgggtatg aaagtgagga aaactataca tttttagagt
    48421 gtaataatca attatctttt ataaagcctg caaattatga aattagtaaa actagaaaat
    48481 ataaaaatga tattgggcgt attgataata tggattatga tgaaaaaaaa gattattata
    48541 tttgtaaaaa tggcaaaaaa ctatttgcta ataaaataat caatagaaaa tcaaaaacag
    48601 gatataaaag taaaataact tgttatactt gtaaagaatg ctgtgattgt caatataaaa
    48661 acggttgtat taagggaaat aatagtaaga ttcctcttga aaatagaact aaaaatttac
    48721 aagtatctaa gttatttcat gaaaaaagaa aagaaaattt aaagagaatt ataagccaag
    48781 aaggttgtca acttagaatg aatagaatta tacaagcaga aggaactttc gctcaagtaa
    48841 aacaaaatat gaaatttaga agatttttaa gctgaggcaa agaaaatgtt atatcagaat
    48901 gtattattgt agcgatagct cataacattt ctagactaca caacaaaata ataaaaaata
    48961 aatgtggaat atatcttcat catttaaaag aagtattcta aatttattat ttatagttag
    49021 atattatctg aaaaaatggt atttttatac ctttatttgt tatgcataat tttacttaga
    49081 acataagctt tatataaaac aattaaaaat atatctaatt aagataagta aaaaaagcta
    49141 tcacagaaca aatttaattt gctaatgcga cagcccattt ttgttaattt gacacttaat
    49201 aatattaaaa taacaaaaaa gtattgaaag tatattgcat ttaaggtaga attaaataaa
    49261 atattataac attataatgt ttatatagtt gttaataaaa atttggagga aaatatatga
    49321 taaaaataat gttggcctgt tcagcaggga tgtctacaag tttattagtt actaaaatgg
    49381 aaagtgcagc taaagaaaat ggtatagagt cgcaaatttg ggcaatacca gaatcaacta
    49441 tacaaaatga aatagaaaaa tgtgatgtat tattactagg accacaagtt agatatgtat
    49501 tacctaaggc tcaagaaatt gctaggccat ataatatacc agtagaagtt ataaatatga
    49561 tgcactatgg aacagttaat ggagaagctg ttttaaatag agcgatagaa cttaacaaca
    49621 ctaagtaata taaattataa tgtattttta atatataaat tgggagaatc aaaggatgga
    49681 tgaaaagatt atagaaatat cttttaatat aataggttat gcaggtgaag gtaaaggatt
    49741 agcttttgaa gcaataaaag aggctaagaa tggaaatata gaaaaagcaa aagaattatt
    49801 aaaagagtct agggaagtta taaataaagc acatagatat caaacagaat taatacaaaa
    49861 cgaagctagt ggaaacagga ctgaaataag tgtcatactt atacatgcac aggaccactt
    49921 aatgaatggc atgaattttc aacagttagc tgaggaaatc gttgatttac atttgaaatt
    49981 acaagataaa taaaaattaa agataataaa ttaagagagt tcttaaaaga tacattatgt
    50041 gtagcacact aattgttaga ttattgattc aatatttagt gagtaataca ttaggattat
    50101 tttgaggact ctttttatat ttagtttaaa gttaataaat atttattatt aataatataa
    50161 gattatatgt tataatatta taacatataa tttaagaggt gaatcatgtt aagaaatcaa
    50221 ccattatata tacaagttgt agatatatta aagcaaaaaa tattaaataa cgaatataaa
    50281 attgatgaaa atattccaac agaaagggag ttagagcaag agtttggagt cagtaaaatt
    50341 actattagaa aagctattga gatattagaa tcagaagggt atgtagagaa aaaaagtgga
    50401 aaagggacaa aagtaataag taatagtctt tttaataagc tatcaaaagc agagtctttt
    50461 tcaagtatac ttttatctaa aggtcataag ttatctaaaa agactatttc aattgaaaaa
    50521 ataataaatg atacaaattc acaagtattt agtatatttg gagaaaaatg tagcaagatt
    50581 actagaatgt attacttaga tagtaagcca tatatatttt ttactcatta tattcctttt
    50641 gatgaaaatt taactgttga aagttttaat aatgaaagtt ctttatatat gtacttatat
    50701 aggagaaata taaatataac tagatttgaa gatgaatttt ttatagaaga atcagaacta
    50761 gaggtatcaa aggctctaaa tctagctaaa gacatattat taggaagaaa aagaaagacc
    50821 tatgatgaga atgataatat agttgagcta tcatttgcaa gatataatac agaagtacaa
    50881 aactatgtaa ttaaatacga aatataataa gaatcctgag tttattgaaa tgataatatg
    50941 ctcaggattt ttgtttagtt gtaaaaacaa aaaaatatta taaca
//
LOCUS       DAGSRM010000020        50693 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.020, whole genome shotgun sequence.
ACCESSION   DAGSRM010000020 DAGSRM010000000
VERSION     DAGSRM010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 50693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50693
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.020"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            148..1245
                     /locus_tag="MJE33_001280"
     CDS             148..1245
                     /locus_tag="MJE33_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBY9647221.1"
                     /translation="MKKTYLFSPGPVMVTDKVRNSLLHYDICHRGNEFMNLFKDTQQK
                     INKLYNATSNYYSLIVSGSGTSVNECVLSSIFDKEDAVLLVSNGVFGERLEEILSTYN
                     VKTYKPNFEWAEYPDLDILEKYIIENSDIKVIAMVFHETSSGMINPVPELGKLAKKYN
                     KIFFVDAISASAGEYIDVEEFNIDIITGVGGKALGAFPGSAYLCAKESILQNIKENQC
                     KNVYLNLYKHYKVAKSSSQTPNTPNVTLIFALNEALTEFLEDDSKIERYKECSAILRN
                     GMEELGLTFLLPDKYMSNTVTSVFLPKEINVNEFILGLERESGYVVYPGKGKFLDANI
                     FQVANMGEIYPDDCYKFLDILKSKLEIKKVI"
     gene            complement(1736..2248)
                     /locus_tag="MJE33_001281"
     CDS             complement(1736..2248)
                     /locus_tag="MJE33_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647222.1"
                     /translation="MKVKKFKSGIVMALTVMAIGASLTNVSAMELNKSKEFETKYNEM
                     KRDFQEEYGKEDKFNITLDDEFTKEEVNGAKGIIKTTNKMNNEVVVLNYFDEMKSGIE
                     FELKPKAEKNAILRDEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     VYNYFDELEK"
     gene            2801..3682
                     /locus_tag="MJE33_001282"
     CDS             2801..3682
                     /locus_tag="MJE33_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HBY9647223.1"
                     /translation="MKKRLIIMILSVVLVLSSILTIFAYSNIKYNNNKLIYSNMIDKK
                     TQNSVKEILKENKINEKDIDTFIKAVNNYNKLQVKILQNNINISKSGYSSINAKQVPY
                     NLEKLQDNWVKKFPDYMDVNCRITAFRLFKDFINSNKKFTGDSIDLNVDLDTIMNNKD
                     AKFSTKDVEKFINFFSAIPAKDTDDTIKIAEQIKNEWKKRKISFKNNKNISIINGFLR
                     YPETKNVFIGHTGICIKTKNGILFLEKYGVTSPYQVTKFKNKEDVKNYMFNRLKMSEG
                     EIELPDPIIMENDKLMK"
     gene            complement(3995..4774)
                     /gene="hisJ"
                     /locus_tag="MJE33_001283"
     CDS             complement(3995..4774)
                     /gene="hisJ"
                     /locus_tag="MJE33_001283"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HBY9647224.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAMDVGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKANL
                     NKYGEELEDSLLSVHIIKIDDDYYCVDYSVEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNTILEELIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLELVKKYDIPLVLGSDSHCAEEINKYDII"
     gene            complement(5320..5670)
                     /locus_tag="MJE33_001284"
     CDS             complement(5320..5670)
                     /locus_tag="MJE33_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HBY9647225.1"
                     /translation="MIFDKEIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            complement(5686..6102)
                     /locus_tag="MJE33_001285"
     CDS             complement(5686..6102)
                     /locus_tag="MJE33_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HBY9647226.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            complement(6121..6312)
                     /locus_tag="MJE33_001286"
     CDS             complement(6121..6312)
                     /locus_tag="MJE33_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HBY9647227.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            complement(6463..7278)
                     /locus_tag="MJE33_001287"
     CDS             complement(6463..7278)
                     /locus_tag="MJE33_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBY9647228.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDK
                     SINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RKIDIDILQKNIVKGLAEQFNVEFVEGDLTDYEKQLVKELYEKYKNEEYNKKR"
     gene            complement(7325..8263)
                     /locus_tag="MJE33_001288"
     CDS             complement(7325..8263)
                     /locus_tag="MJE33_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBY9647229.1"
                     /translation="MIRLSVGTAIELGILNKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVIWPEFSKEEILDALKAYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGKIDMPISLSAKLENDEQIKKFFSAGVNKIGIAIDAANKELYEKIKGNNYDEKLKF
                     ITEMSKSYPNKISTHIIVGMGESHEDIYNLYTYLKENDVTISLFAFTPVRGTKMEKIS
                     QPSIESYRRVQLMSYMINKGYPKEYFKFKNGYLNSIKLDNDILKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            complement(8260..9132)
                     /locus_tag="MJE33_001289"
     CDS             complement(8260..9132)
                     /locus_tag="MJE33_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBY9647230.1"
                     /translation="MNKNLSEKMKIAFDIKLKNFGNKIEFAYPNQTLALSTTSNQCSL
                     KCAHCNGHYLNNMVPIEEYEEKVQSRNITSFLLSGGCSYEGDVPINTHINTIKNLKEQ
                     GYRLNAHLGLMDKDSIVELCKYLDIVSFDLVFDDETIREVYKMKKSKEDYIEVYNTIQ
                     EHTEVAPHICIGLKGGQIKGEYEIIEYLQKNPPNKLTFIVLIPTKGTEYENVEPPELE
                     GVADILCEARINLPDTEINLGCMRPRGVYRKELDQLSIMCGVNRIVLPSRSAKNKAIE
                     MNMTINECKECCVL"
     gene            complement(9157..11085)
                     /locus_tag="MJE33_001290"
     CDS             complement(9157..11085)
                     /locus_tag="MJE33_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4445 domain-containing protein"
                     /protein_id="HBY9647231.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNNL
                     GYNEIDFRLDILRKMPTVFRKSDFKVTITYVQKQKKLTIINIEQGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKDTLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGVIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            complement(11197..11574)
                     /gene="gcvH"
                     /locus_tag="MJE33_001291"
     CDS             complement(11197..11574)
                     /gene="gcvH"
                     /locus_tag="MJE33_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HBY9647232.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            complement(11661..13787)
                     /gene="cdhC"
                     /locus_tag="MJE33_001292"
     CDS             complement(11661..13787)
                     /gene="cdhC"
                     /locus_tag="MJE33_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HBY9647233.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVITDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            complement(13844..14650)
                     /locus_tag="MJE33_001293"
     CDS             complement(13844..14650)
                     /locus_tag="MJE33_001293"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBY9647234.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            complement(14700..16067)
                     /locus_tag="MJE33_001294"
     CDS             complement(14700..16067)
                     /locus_tag="MJE33_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HBY9647235.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDAKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSGLEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRTALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            complement(16098..17042)
                     /locus_tag="MJE33_001295"
     CDS             complement(16098..17042)
                     /locus_tag="MJE33_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HBY9647236.1"
                     /translation="MAFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NSPKIGIQISDVYPESWTDSYKELYKDVANCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYAHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            complement(17062..17832)
                     /locus_tag="MJE33_001296"
     CDS             complement(17062..17832)
                     /locus_tag="MJE33_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBY9647237.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            complement(17888..19273)
                     /gene="lpdA"
                     /locus_tag="MJE33_001297"
     CDS             complement(17888..19273)
                     /gene="lpdA"
                     /locus_tag="MJE33_001297"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBY9647238.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVVEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTVKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            complement(19317..20198)
                     /locus_tag="MJE33_001298"
     CDS             complement(19317..20198)
                     /locus_tag="MJE33_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HBY9647239.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKPLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMKKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRAAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            complement(20234..20908)
                     /locus_tag="MJE33_001299"
     CDS             complement(20234..20908)
                     /locus_tag="MJE33_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HBY9647240.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKILGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKDKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNSENDCAWIKIYERLEAIGQLDNLAEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            complement(20943..21815)
                     /locus_tag="MJE33_001300"
     CDS             complement(20943..21815)
                     /locus_tag="MJE33_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HBY9647241.1"
                     /translation="MEGMSTKGQIIKGKPVADKISEELIKEVDLLVKEGINPKLTIVR
                     VGARSDDLSYERGALKRCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCF
                     RPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
                     DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRA
                     KMVDESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSIL
                     AKHVVKACKLQNNK"
     gene            complement(21907..22539)
                     /locus_tag="MJE33_001301"
     CDS             complement(21907..22539)
                     /locus_tag="MJE33_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HBY9647242.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVNKVKTEVDKLVEDGVKICDEVYAKVEKALGK"
     gene            complement(22644..24320)
                     /locus_tag="MJE33_001302"
     CDS             complement(22644..24320)
                     /locus_tag="MJE33_001302"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBY9647243.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNNENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKELGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLEKLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            complement(24569..25348)
                     /locus_tag="MJE33_001303"
     CDS             complement(24569..25348)
                     /locus_tag="MJE33_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBY9647244.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVAVDADPDANLAL
                     ALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPKVDDIPENFCKEYNGVR
                     LLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAGIEHLGRGTAQGVDA
                     FIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEEFIIQNLEDGESLGF
                     IYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
     gene            complement(25658..27577)
                     /gene="cooS"
                     /locus_tag="MJE33_001304"
     CDS             complement(25658..27577)
                     /gene="cooS"
                     /locus_tag="MJE33_001304"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBY9647245.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKAGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMESDAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTSELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMTEKAEA"
     gene            complement(28184..29095)
                     /locus_tag="MJE33_001305"
     CDS             complement(28184..29095)
                     /locus_tag="MJE33_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBY9647246.1"
                     /translation="MIKKLDDILEQLKGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKAKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGENVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMADSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            complement(29211..29789)
                     /locus_tag="MJE33_001306"
     CDS             complement(29211..29789)
                     /locus_tag="MJE33_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBY9647247.1"
                     /translation="MNINEFLTHINQRKPVSSKSEIHEFMVKLSNEAMKITSKLNSSY
                     HEPEEVRSLFSELIGKKVDESFFMFPPFYTDCGKNITIGKNVFINSSCHFQDQGGIDI
                     GDNTQIGHNVVLATLNHGIAPEKRGTTYPSPITIGKNVWIGANVTIVPGVTIGDNVII
                     AAGAVVTKNVAENTIVGGVPAKLLRVIDEIPE"
     gene            30015..30938
                     /locus_tag="MJE33_001307"
     CDS             30015..30938
                     /locus_tag="MJE33_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBY9647248.1"
                     /translation="MEIRVLRYFLAIAREESISGAATFLHITQPTLSRQIMELEEELG
                     VKLLVRGNRKITLTNEGRLLHKRAEEIISLVDKVELEFDNPNEIISGDIYIGSGETEA
                     MRLIARTAHHFHQKYPNVRYHLFSGNADDVTEKLDKGLLDFGILIEPANIQKYDYAQI
                     PATDVWGLLMRKDSALASQSTINPKDLCNIPLIISRQTLVDKAISKWLQDSNEKLNII
                     ATYNLVYNASLLVEEGLGYALCLDRLVNTTENSNLCFRPLSPPLEAKLNIIWKKHQIF
                     SKAAEKFLLQIQKDFSSIMLDTSKLDTPKEK"
     gene            complement(31110..32252)
                     /locus_tag="MJE33_001308"
     CDS             complement(31110..32252)
                     /locus_tag="MJE33_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBY9647249.1"
                     /translation="MDFKIEKYLLKKTEELAFITIKHGGEFKLKSYEVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     SYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEKVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALRLGLDAD
                     LVAEVQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMGEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELGINK"
     gene            complement(32270..34024)
                     /locus_tag="MJE33_001309"
     CDS             complement(32270..34024)
                     /locus_tag="MJE33_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HBY9647250.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVNMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYEAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMEINDVNGLVYRQGNEIIVNKPMELLQNLDE
                     IPSPYENLNPKEYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLDRIKSDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKKSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNIPQFFNKLDVDDFKNRC
                     HVFLQDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREVENVVLF
                     EYDDKKIFEKSKTHKVEI"
     gene            34160..34366
                     /locus_tag="MJE33_001310"
     CDS             34160..34366
                     /locus_tag="MJE33_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647251.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            complement(34371..35294)
                     /locus_tag="MJE33_001311"
     CDS             complement(34371..35294)
                     /locus_tag="MJE33_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647252.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLTIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVIYFKKE
                     ELLFDYLLGSVVYNSIMHNLINNSKIEYVELLQSIKDKIIGFSIELDKSDVVKFQMAR
                     IKAIQLIDKYIDLKSEDYDEESILLNVLNVLYDVYMEDRIVENEGINSIKKSILSILG
                     EDSKLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNKSIDPRSLIRLEEGNTIIDPIFNQ
                     ITVMSKTFNDNILSIKINSKSGIYILKFKKV"
     gene            complement(35549..37249)
                     /locus_tag="MJE33_001312"
     CDS             complement(35549..37249)
                     /locus_tag="MJE33_001312"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBY9647253.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNK
                     LENGDEEAQELWQWFRNESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKN
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKEIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGMHAPERM
                     "
     gene            complement(37712..38125)
                     /locus_tag="MJE33_001313"
     CDS             complement(37712..38125)
                     /locus_tag="MJE33_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBY9647254.1"
                     /translation="MILVSACLIGINCKYSGDNNDNEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLKIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEEGIKVKSEKDL"
     gene            complement(38191..40569)
                     /locus_tag="MJE33_001314"
     CDS             complement(38191..40569)
                     /locus_tag="MJE33_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBY9647255.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     IAQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKSEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSELSAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPADYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTADSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKSGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIEKLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRSLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTRKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            40888..42339
                     /locus_tag="MJE33_001315"
     CDS             40888..42339
                     /locus_tag="MJE33_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HBY9647256.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLVTVNEEDGMNGADALDPSL
                     IKGQYILNMDSEEEGYLLVSCAGGKTCVVSLPVEYKEVKGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLNVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINDILTAFKHEFTTSDPGMTYSVAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKGSKLEKICSETYEKLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANVWDYVIEILKSMNQY"
     gene            complement(42543..45056)
                     /locus_tag="MJE33_001316"
     CDS             complement(42543..45056)
                     /locus_tag="MJE33_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBY9647257.1"
                     /translation="MEDCVVNLKKLLLLIIVITSIFTSCIFSFGINAIGNDNRTIRVA
                     YPIQKGLTEIDEQGNYFGYTYEYLLEIAQYTGWNYEFVQVPGEINESITKLMKMLENG
                     EIDIMGGMLYNEPMQKIYNYAGNNYGVANTVLQALYENTDIDNIMSSQDVKKIRIAVI
                     ENSDTRLKELDEYCKMNLMSPELVYCKTNDEQLEALKSGKADVLLNVSMNPIEGVRTV
                     AKFSSKPFYFITPKGSTNLTQKLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDE
                     ETNYIKETEKLKVGVLLNKPPFQNIDKDTGELRGISIDLLNHICDKTGLEFELVPVKS
                     QEDLENMTKKREIDIVAGMIYDYEYSREHNIAMSRPFLSTQYTMMINNKLNENSLDGK
                     RVALAKGINYYGQFEENIVWYDNLEECIEAVNKGYADYTYGEGYSMQYYMNQPKYKNV
                     RLVPQTYKAKSICFGIIKKDKQELLNIFNKAIISISSEKMQSIVYQNTTYRQDFSLLY
                     IMQQNPIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFE
                     YDYKQDKLIISMPEKGIETYYGLDKNDKESENGNIENERDKIRKEYNKKFFDVIKSQE
                     NGTTEMYSIMLDDTFHWIRVTSKIIYNDRKIPMYVIGKICNIDSEKEEKNRLLDKAQR
                     DSLTNIYNAAYIRKLTAQNLLNLSTEKNGALLIIDIDYFKSINDTYGHLLGDKVLIDV
                     AKVLDESFAKNDIVGRLGGDEFSVYINDIINKEELINKCNDVYNHIHLIKLPNGESLS
                     ISMGVVIAKPGQEYDELYQIADTALYDSKRQGRNRFKIV"
     gene            complement(45279..46100)
                     /locus_tag="MJE33_001317"
     CDS             complement(45279..46100)
                     /locus_tag="MJE33_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBY9647258.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVIEYNRESFELEPEKLLNFIKFRRSIRQYKDIEVEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIQGLYNLALDTDDNDPVRSMYKNT
                     FKKMYKRYKENGNDSLFYDAPLLMVVVGDMSLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEVR"
     gene            complement(46619..47095)
                     /locus_tag="MJE33_001318"
     CDS             complement(46619..47095)
                     /locus_tag="MJE33_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HBY9647259.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVDNDKIFDLNMWEGDRLFLNWAKTGNIFSAKIVYDNGKLKDYNVSFLD"
     gene            complement(47130..47708)
                     /locus_tag="MJE33_001319"
     CDS             complement(47130..47708)
                     /locus_tag="MJE33_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBY9647260.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRELAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGDEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            complement(47812..50412)
                     /locus_tag="MJE33_001320"
     CDS             complement(47812..50412)
                     /locus_tag="MJE33_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBY9647261.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     RNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELNVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIEAGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDKKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKDGEITTIAHKGDLIELDFVGEAKRNQLIYKTSDSEL
                     MDRVQATFQQDKEFIKTNIRAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDEERIKIKD
                     RKFKKNRLKYQPNRVNKQKKLDTAPKIRVKVKNLEQLEAVLNYNVDLIYYEDINTLSK
                     ALQMVSSISIDSNISIDSSISMDLDTSSSLGTPKELMGSNTNNIDTLSNTSKIDTLNK
                     NHYNTAVTDKKLIYSAPRIVRNKEYKQFEILDNIKGINKVQIGNWGSMEYFKKKDLNI
                     DCYLNAFNSESINHFKEEGASTVCLSQELNLNEIKQTLKYTDVEIEAVIYGYAPMMVT
                     EYCPMGVVVRDCKKDKRVAKCKESSYALRDFTDASYRITQDIFCRSTIYNSKVTCMLD
                     NLYELEEIGIDVFRIDFTVEDSEMVKKVIEAHIEVLSNNYKLGKKATDLYKKLDEIGT
                     VAGHYYKGVE"
BASE COUNT        15652 a         9564 c         5677 g        19800 t
ORIGIN      
        1 tgattaaaaa agtattttta ttgaaatatt attgcaatat ttattatttt attctataat
       61 ctagttaaaa taacacataa cattttaata tttacacata atcagtcatt caaaaattta
      121 aatttaactt tttaaggagg ctttacaatg aaaaaaacat atctattcag tcctggacca
      181 gtaatggtta cagataaagt cagaaattca cttcttcact atgatatatg ccatagagga
      241 aatgagttta tgaatttatt caaagataca caacaaaaaa tcaataaact atataatgca
      301 acttctaatt actactcatt aattgtatct ggctctggaa catctgtaaa tgaatgtgta
      361 ctatcttcta tctttgacaa agaagatgca gttctgctag ttagtaatgg tgtttttgga
      421 gaaaggctag aagaaatatt atctacttat aatgtaaaaa catataaacc taattttgaa
      481 tgggcagaat acccggacct agacatttta gaaaaataca taattgaaaa ttctgatata
      541 aaagtaattg ctatggtgtt ccatgaaact agttcaggaa tgataaatcc cgtccctgaa
      601 ctaggaaaac ttgctaagaa gtataacaaa atattttttg tagatgcaat aagtgcctca
      661 gcaggtgaat atattgatgt ggaagagttt aatatagata taataacagg tgttggtggc
      721 aaagctcttg gagcatttcc aggttctgca tacctttgtg ctaaagagag tattttacaa
      781 aatattaaag aaaatcaatg taagaatgtg tatcttaatc tttataaaca ctataaagtt
      841 gctaaatcat cttctcaaac accaaataca cctaatgtta ctttaatatt tgcccttaat
      901 gaagctttaa ctgaattttt ggaagatgac agtaaaatag aaagatacaa agaatgttct
      961 gctattttaa ggaacggtat ggaagaattg ggtctaacat ttttacttcc agataagtat
     1021 atgtctaata cagtaacatc tgtatttcta cctaaagaaa tcaatgtaaa tgagtttata
     1081 ttagggctag aaagggaaag tggctatgtt gtctatccag gaaaaggcaa attcctagat
     1141 gctaacatat tccaagtagc aaatatggga gaaatatatc cagacgattg ttataaattc
     1201 ttagatatct taaaatcaaa attggagata aaaaaagtta tataacagtt tgtttgtaaa
     1261 atcctacaat atatcagtat aaattaaata gactgatttt tttaaatttt cctataataa
     1321 attatatttt atatttttgt ataagggtga actataatat gtaaagttca cccctatttc
     1381 aaatcttttt ccttcaatat aatctttttc ttttaattta ttttatattt ttgacgcaat
     1441 cgttttttat tcaatcattt tatattcttg atacaatcat tcttacactt aattattttt
     1501 tgtttttgac acaatcgttt tttgttgaat cattttatat tcttgataca atcattctta
     1561 cacttaatta ttttttgttt ttgacacaat cgttttttat tcagtcattt aatattttca
     1621 acacaatcat tcttacattc tattattgtt tgtttttgat acaactgttt ttattcagtc
     1681 atttaatatt ctcaacacaa ttaccttttc attcaatcaa agtaatcttt aagcattatt
     1741 tttcaagctc atcaaagtaa ttatatacga taacctcacc tgtctcttta tctgtagtct
     1801 ttataatgcc cttagctcca tttacctctt cttttgtaaa ttcatcatct aacacaactt
     1861 tgaatttatc ttcctttcca taaccttgtt gaaattcatc tctcaaaatt gcattttttt
     1921 ctgcttttgg cttcaactca aattcaattc cagatttcat ttcatcaaaa taatttaata
     1981 ctacaacctc attattcatt ttattagttg tctttataat ccctttagct ccatttactt
     2041 cttcttttgt aaattcatca tccaatgtta tattaaattt gtcttctttt ccatattcct
     2101 cttgaaaatc cctcttcatt tcattgtatt tagtttcaaa ctctttactc ttgtttaatt
     2161 ccatagctga tacatttgtc aatgatgctc caattgccat tactgttaat gccattacta
     2221 taccactttt aaattttttt actttcatat cttacctcct aaattaaatg ttctcaatga
     2281 aattatataa agaaaatcct aagttttaca gtttcattct tgtaactaaa attactatta
     2341 tgtaaccaaa ctttttgctt caactattcc ttaaaagttg cttttttgag gaatagttga
     2401 agttaaataa ttagatttta tctatattta tagatttaaa atcaaattta tccatataaa
     2461 taaatactta tttttgcaaa ataaaaattt ttaattactc caatccttct tatttactta
     2521 aaattctatg ataagaaatt taaaagtctt taccttcttt aacaataatt tatttacttt
     2581 taacatacaa aatagagaga gtatatcaaa atcattttac tgaatattct aaaaataagt
     2641 accccctatt agtatatata tttacatatt aaaaaaaagt tttaaataaa gttatatttt
     2701 ttacaacagt tttgtaatta aaaacatatt gaaggtaaat ttaaaggtac tatatactat
     2761 aaatatgtcg acacaataaa tttaaaaagg agaactaaat atgaaaaaaa gattaataat
     2821 aatgatattg tctgttgtct tagtactatc aagtatactt acgatatttg catattcaaa
     2881 tattaaatat aataataaca aattgatata ttctaatatg atagataaaa aaactcaaaa
     2941 tagtgtaaag gaaatcctaa aagaaaataa aattaatgaa aaagacatag atacatttat
     3001 taaagcagtc aacaattata ataaattgca agttaaaata cttcaaaata atataaatat
     3061 atctaaatct ggatattctt caatcaatgc aaaacaagtt ccatataacc tagaaaaatt
     3121 gcaagataat tgggtgaaaa aattccctga ttatatggat gtaaattgta gaataactgc
     3181 ttttagactt tttaaagatt ttataaattc aaataagaaa tttactggag actctattga
     3241 tttgaatgtc gacttggaca ctataatgaa taataaagat gctaaattta gcaccaaaga
     3301 tgtagaaaaa tttattaact tcttttctgc aataccagct aaagacacag atgatacaat
     3361 caaaattgca gaacaaataa aaaatgaatg gaaaaaaaga aaaatatctt ttaaaaataa
     3421 taaaaatata tctattataa atggatttct tcgttatcca gaaactaaaa acgtatttat
     3481 aggtcataca ggcatatgta ttaaaactaa aaatggcatt ctatttttag aaaaatatgg
     3541 tgttacatca ccttatcaag ttactaaatt taaaaataaa gaagatgtaa aaaattatat
     3601 gtttaataga ttgaagatgt cagaaggtga aattgaatta cctgacccta taataatgga
     3661 aaacgataaa cttatgaaat aataacttta aacgcaaaat agtatttata attactaagt
     3721 caatataata gagaaggtct atagatttgt gtaaatacaa gcttatctac aatcttatat
     3781 aagaatgttt atataaataa gatagattaa tcctaagtcc tattattcta ttgaatttta
     3841 ttattatttt atttagattc tattgttatt ttacttagaa cttattatta ttttatttaa
     3901 gttctattac tattttcaat catataaatt tacaaaagac tgtacataaa tttattttat
     3961 acaaacttat ctacagtctt ttgtgtgaat atatctaaat aatatcgtat ttatttattt
     4021 cctctgcaca gtgagaatca gaacctaaaa ctaatggaat atcgtatttt tttaccaact
     4081 ctaataagta tccatctata taaggctctt tgcagtactc ttttctattt ccagaaacat
     4141 tataatcaag ttcataacct ttttctttaa ttaactttat taactcttca agaattgtat
     4201 ttcctttata atcgtatgga aatacctgat taaactttct aactaaattt aaatgaccta
     4261 ttctttttgg tttatgtata cctaaatctg aatttacagc ttttatcaat gtttcataat
     4321 acttattata aacagcttca atacttccta acttgtcgat taaattttga aactcctcta
     4381 cactgtaatc tacacaataa taatcatcat caattttaat tatatgtact gataaaagag
     4441 aatcctctaa ttcttctcca tatttattta aattcgcctt tatcccttct tcgtatcctt
     4501 ctataaaatc cacttccgac ccaacattta tcttgatttt accctcatac ttttctttca
     4561 acttcttgac atcattaaaa tactttggta aatcttcaat actcatacaa ctatcatttt
     4621 caggagcagg gtctctaaat ccatttgcat acggaaaatg ttctgtaaat gtcatttctg
     4681 ttattccaac atccattgct ctttgtacat atttttcaaa atcatcctta cttccatgag
     4741 gacaataagg tgaatgtata tgaccatctt tcataaataa ttcactcctt ttttaaagaa
     4801 aaaatctaat ataaatgcat aaatctttta acttcaaaat aatattttta atcatgctaa
     4861 tttttattat atctaatatt agagtaattt taaactgcct tttaagtatc ttatcattag
     4921 tactattttc aacttaacat taatattatt ttttaactta acattaatac tatctttaaa
     4981 tagtaatact caatatataa ttacttaaca taaaataact caaattgctt gatgaggctt
     5041 ttgcagtatt taaagaatat ataattattt aacattaaat tatccaatct tgtaataaaa
     5101 tttactcatt aaagtatatt acagaaaata cctcaaaata aactttttag ttcatgagat
     5161 actctttttt acatgaaatt agatatattt ttatcatttc aacaatgaaa gggaggctta
     5221 tatccatctt tagacaaccc cttagataaa atttttaata attaagcatc acaaaatata
     5281 aaaatcgctt atatcaaacg tataagcgat taattatatt tacatatgtt ttttagcctc
     5341 taacaacatt ttattagctt cttccatata ctctatagcc ttttcaatac tctttgctgt
     5401 ttcttctttt ccaatagctc tagccttttc aacccactct ctaaatcctt cttcatgagt
     5461 ttcattgtga tttatccaat gtactaaaag tatttttaaa gttttttcat ccttagactc
     5521 tgatgattcc tcatgactat gttcgtggtc gtggtcgtga gcatggtcat gtggatgttc
     5581 atgacatata tctccatgag tatgttcgtg gcagtggtca tggtcatgat gtccatgatg
     5641 atatcttaca atctctttat caaaaatcat tagttattcc ccctgttatt ttatattaaa
     5701 ctcttttttt ataaggttta cagaatcttt tacaagtagt gctaatggtt taccttctaa
     5761 tccaactaac tccacatttt ctggcataat aggtaaaagt atcttaagtg cctcactgtc
     5821 tgcaatagct tcacttatat tacaagtaac ttctcccatc atagaattag gtattattac
     5881 agaaataggt gcaactatta tatttgcttt tttagctgat actactatag cattttcccc
     5941 tgtagctcct ttattagcat gagctttcat catagaacta gttgctatag aattagtccc
     6001 tagtgcataa atttctacat atgtaggtaa ctcttctctc aaagaagaaa ctatctgggc
     6061 tccaatacct ccacccattc catctatcac tgctattatc atatgaaatt cccctattaa
     6121 ttaattttct tgtattatta tcttatggtc catcaatctt atttctttaa ttatcccatc
     6181 tattatcttt tgctctccta ataaatccgt taaataaatc tttccatcac aagggtctat
     6241 attaaccaca ttttccataa ccttttctaa ctcattatta cttttacaaa tgaaagctga
     6301 tgattcacac atataaatct cctcctaata tgatttttat taaaaatcaa aaaaagattc
     6361 ccttaggtat ttcaatacac caaagaaacc ttcatgattt cagtttttgt atattattat
     6421 accttaagta tatcacttta atttatgtaa agtcaattaa agttaacgct ttttattata
     6481 ctcttcattc ttgtattttt catatagttc tttaactaat tgcttctcat aatcagtcaa
     6541 atcgccctcc acaaactcaa cattaaattg ttcagctaat ccctttacta tgtttttctg
     6601 tagaatatct atgtctattt ttctgccaat ttcattttct attccacttg cttttttagc
     6661 tgtaaatttc atagctcttt ctttcaattc tggtgtctta gtttttatta atttaaacaa
     6721 tttttctaca tcaaaatcca gtattattga accatgttgt aataaaacac catcttttct
     6781 gctttgagca cttccaatta ccttcttatt attaatagta acttcatatg atgcatgtgc
     6841 attaaaacat gcagctgata aattttccct acttattctc tcgcctctat ttaaattatc
     6901 tgtttctata ccacttagat tcaacccttt aactagacct tcacttatat atctataaga
     6961 taggttaatg ctcttatcga ttaaaggatt atcttctcct ataattatac tataggttag
     7021 ctcattatca tgtaatacag ctcttccacc tgtaattctt ctagtatagt ctatattcat
     7081 acatctacac ttatctaaat ctatctcatc ttccaatttc tgaaaatatc ctatactcaa
     7141 acaagctggt tcccatgtat aaaatctaag agttggctta ttatgtcctt tcttatatgc
     7201 agtaaatata gcttcgtcaa ttgccatatt cattgctcct tcatatgatt tattatgtat
     7261 aactctccac tgattcatga ttattacgcc cccattttta gtataaatat atcctagaaa
     7321 tcctctatag atttatttct cttattgcta aatctatctc actttgattt aaaggtcttg
     7381 aatagttgta tatagtactt cctggtcttt cattatagta tggtctatta caatccttac
     7441 aacctcttat ttcaaaagga tagcctttgt ttatgtcttt taaaatatca ttgtcaagct
     7501 ttatactatt taagtatcca tttttaaact taaaatactc ttttggataa cctttattta
     7561 tcatataaga cattaactgt acccttctat aactttctat gcttggttga cttatctttt
     7621 ccattttagt tcctctaact ggagtaaatg caaataaact aatcgttaca tcattttcct
     7681 ttaaataagt atataaattg tatatatctt catgactttc acccatacca actattatat
     7741 gtgtacttat tttattggga tatgattttg acatttcagt tataaatttt aacttttcat
     7801 catagttatt tccttttatt ttttcatata gttctttatt tgctgcatct attgctattc
     7861 ctattttatt tactcctgct gaaaaaaact tctttatctg ttcatcattt tctaattttg
     7921 cagatagtga aattggcatg tctatcttac cacttatata gtttataaag tctaaaacag
     7981 agttatgtgc ttcttcactt gccattgatt gaatacatat tctctttata ttttttcctt
     8041 tataagcttt taaagcatct aatatttctt cttttgaaaa ctctggccaa attactcttg
     8101 ataatttatc ttttctagta ctactttcta ttgattgaga acaaaaactg catttattta
     8161 tgcatttttc tccaatcatt atatatgctg tggtaggagg tatatcgctt tttttgttta
     8221 atatcccaag ttctattgct gtcccaactg atagtctaat cataaaacgc aacactcctt
     8281 acactcattt attgtcatat tcatttctat agccttattc tttgcagacc ttgatggtaa
     8341 tactattcta ttgactccac acattataga taattggtct agctcttttc tatacactcc
     8401 tcttggtctc atacaaccta gattaatttc agtatctggt aaattaattc tagcttcaca
     8461 taaaatatct gctacccctt ctaactcagg aggttctaca ttttcatact cagtcccttt
     8521 tgttggaata aggactataa atgtaagctt attaggagga tttttttgta ggtattctat
     8581 aatttcatat tcacccttta tttgtcctcc ttttaaacca atacatatat gtggtgctac
     8641 ttctgtatgt tcttgtattg tattatagac ttcaatataa tcttctttac tctttttcat
     8701 cttataaact tctcttattg tctcatcatc aaacactaaa tcaaaagaaa ctatatctaa
     8761 atacttacac aactctacaa tgctatcttt atccataaga cctaaatgag cattcaatct
     8821 atatccttgc tcttttaagt ttttaatggt attaatatga gtatttatag ggacatctcc
     8881 ctcataacta caaccgccac tcaatagaaa gctagttata ttccttgatt gtactttttc
     8941 ctcatattct tctattggca ccatattgtt taagtagtga ccgttgcaat gggcacattt
     9001 caaactacat tgattgcttg tagtactaag agctagtgtc tggttaggat aagcaaactc
     9061 tattttattt ccaaaattct ttaatttaat atcaaatgct attttcattt tttcagatag
     9121 attcttattc atagtttatt ccttcttaag tacattttac tcttctagta atttctttgc
     9181 tgttgggaac tgctctatat tcgtatgtgg taagaaacat gcagaaataa attcatccat
     9241 ataacttgga tatacactaa gttcaacata agttatttga ttacctattt cttcaagttt
     9301 attttttgca tctttactta taagagctaa gtaagaacca acaagtgaac tattccctat
     9361 ataagtaaat ttttctctat cgatatcagg aagtaaccct ataattattg agttttctat
     9421 gtctaggttg tttcctatac cacctgctat ataaacccta tctaatatac taaaatccat
     9481 acctaaacta tctacaagag tgtatgcgcc tgaatatata gctcctttag ctctgatgaa
     9541 attatctata tcaacttcat tgactactat atcattttct aaatcaaatt cttctttaaa
     9601 tgctaataca tactctccta tctcatgctc attaaatcta actctcttat tatctaaatc
     9661 cctgtatatt ttacctcttc tatctattac acctttagta atcatttgac agattaaatc
     9721 aattatacca gaaccacata taccaactgg tgcacattca tcaataatct ttaatgttgg
     9781 ctctaaagta tctttatcaa taattacttt ctctattgct cctgctgatg ctctcattcc
     9841 acagcttatc cctccacctt caaaagcagg tcctgctgaa catgcacagc tcatcatata
     9901 atctttattt ccaaatacaa tctctccatt agttcctaaa tctataaata aaacattttc
     9961 ttcacttgac catatccctg ctgataatac tcctgctgtt atatctcctc ctacataact
    10021 tgcaacagat ggagctaagt acacatatgc actgtcattt acaaaaagac caacattttc
    10081 acccataagt tttggagatt ttaaaaatgg tggtatgtaa ggttcttgtc ttaagaagtc
    10141 tgtatacaca cccaagaata aacttgtcat agttgtattc ccagcagcca ctaatgtaac
    10201 tacatcttcc ttatctattc catttctttc atatattgtt tttaatagtg gatttatagt
    10261 ttcctcaact atagctttat gaagtgtctc taatccattt ttcttagttg aatatataat
    10321 tctatttata acatctgctc catattttat ctgtgcattt ccagaagatg ctttatctac
    10381 tacctctttt gagtataggt ctactaaaca aacaactact gaagttgtac caatatctat
    10441 tgcaactccg tataaggaat tttctttatt tccttgttct atatttatga ttgtaagttt
    10501 cttttgtttt tgaacatatg ttattgtaac cttaaaatca ctctttctaa atacagttgg
    10561 catttttcta agtatatcca atctaaaatc tatttcatta tatcctaggt tatttctaac
    10621 atatctttcc aatctatcta catcacttat attatcatct aaatttggtt cttccatttc
    10681 tatgtatttc ttttttatat ttgttttaaa ttgaaggtta tgttcttcaa ttattttttt
    10741 agctctttca aaaatttccc tatcctcttt tttattacta ccttctatct tcataccatg
    10801 catagaggat gatactttag aaggaacttc aatttctata tctgatatta cctttgtaca
    10861 acaagctagt atataacctt gctcccactc atcatctgtt atatgtcttg ttttttctgt
    10921 atcaactttt ccattcaata attttacttt acacttccca caagacacgt ttccattaca
    10981 aggagcatct atgaatatat ctgcatttct tgcaacctct agtaatatat ccccctcatt
    11041 acaataaacc tctttattgt ttggtgtaaa acttactttt atcatgttat ccctcccaat
    11101 ttgttctata tatgataaaa ggaaagtata gctaaatttg cgtgcactca aattttacta
    11161 tactttcccc aattataata atatcttaat ctttaattat tctaatccag cctcgtattc
    11221 tttatcactt aataacgtat caagttcagc cacatcagct agttttactt ttaatatcca
    11281 gttttcatat gggtcttcat ttatacattc tggctcatct tctaattttt cattaacttc
    11341 caatacctca ccattaacag gagatattaa atcagaagct actttagaag actcaactac
    11401 tccaaaatct actcccttag ttacagtatc ttctacttct ggtgtttcaa caaacaatat
    11461 ttctcctaat tgatcttgag catagtctgt tatacctata tatacaacat ctccatcaat
    11521 tacttttacc cactcatgat ctttagagta ttttaattct ggtaataatt tcatttcgat
    11581 agacccccaa tattatatta ttgattaaat tacttaaatc atattaatat tatatcatat
    11641 tattttaaat tttccatcaa ttacattaca ctttccatag ctaatgctgg atgacctttt
    11701 tcttctaaga aagctaatac tccttcagaa tcgtcagcta tagtttcatc acaaaccata
    11761 tcacagaagt tttctattcc agtcatttct ttaactgtag catttaattt atcagctaca
    11821 taatctttta attcttttgg catccaaact attctttctg gtccaccttc tgcataagcg
    11881 aattttttag aagatatgaa atgtcttccg tgtcccataa atcctggagt ttgaactcct
    11941 ccacctgtca tagaagcaag ttctccgaaa gtcataccaa ctggagtaac acttgcaaat
    12001 tctctgttaa ctacaacaaa tccatttgct tctggcatta taccacagat acattcgaag
    12061 catccacaag aagtcattgg gtcttctagt atagagtata atgtaacaga ttcaactgca
    12121 ccttgagata tttggtttac agtttcattt actgaattcc atacacctgt tctatcatct
    12181 aagcactcgc ctttttctat tggttgacaa ggacctgttg ggtctaattc tttagtagcc
    12241 ttagcatcta accagctaac tgctccacaa agaccaagtc tttcaggagt aacaacacaa
    12301 acgtgagctg gtgcaaatga ttgacataag ttacaagaat agaaagtgtc aacactttca
    12361 tcaactaatg aagctaatct ttcatctcta gcattgtact tagctattgc ttcacctttt
    12421 aattcagaaa ctttttcagc atctgtaatt atagttattt cacacttatc aactactgat
    12481 tcaaattcat ctaacatctt agcatataaa acttctccta tatgctcaag tctaaatcct
    12541 ttatcaaatg catctttaga gattcttacc caagccatat ctctttgtcc aacgtgcatt
    12601 acaccttcta tatagtttaa gaagtagtgg aaacgtcttt caagaactgg ctcgaaatct
    12661 tcttgcatat ttttaccagc tattttaact attacagcaa gtggtaatct taatacacca
    12721 tctgcatcaa cttcatctat atttggtcct attatttcta tcttatggtc ttcaacttct
    12781 gttgcttctt tcttaacaac aagctcccaa gcttcagttc tgtttccacc aaactcagca
    12841 aacatatcgc tctttctaat tctctcacct tcaaatgctg ctgcaaatga aactgggata
    12901 ggtatttcag taacttttat ttttattcct ctagcttcta atgaagtagc tactatctta
    12961 tcgtaatctt tttgagttaa taggttcatt ggaacttcaa gaactgtttg gtcagttata
    13021 actgggaatc ctaaagctat tgctccagca cctgcagata caactaattc acttaatggt
    13081 ccaaatgcat taacaaatgc aggaactcta ttagctgtgt actctaataa tccgtttaaa
    13141 tcaccaggag ttaatccacc gaatattaat gctgctctta ctgcaacaga tacaacgtgt
    13201 ataacagaag ttacatcata tcctagaggt ataactctta gctcaagacc catttttact
    13261 ccagcttcta tagcttggtc tataacttta ccaactaaga aagttagtaa acctttagat
    13321 tggtaatctt ttataacttt tgctgctgat tctgcatctg gacactcacc aagaacaact
    13381 gcaacaccag gtatatctcc tgtaactaat ggtacaccaa gtgatctgat tatagggtca
    13441 gttatatgac ctgcacatgg ctcactatat ggagcatcca tagttgaata ttttaatgct
    13501 tcaattactt ctgctgctaa agctgtagct aaaccagcat taaaagcatg ttctagtaaa
    13561 tgagttctat ttattaaaga ttttactact tctaaagcac cttctaagtc ccctaaagtg
    13621 ttcatctttt gtcctgttgc agcatatata caaggtaatg aatatgctgt atcagggaaa
    13681 gcaactttat gctcttttcc attcttttct atagcttcag ctaacatagc ttgagccgca
    13741 cctaaagctt gttctgaccc tgtaaagatt atattatata gattcattct atttccccct
    13801 ttaatattta cacctatttt ttattaacta taccctaaaa accctagaaa gcaaatccac
    13861 cttcatttaa ctctaagaaa gagtctgcat ataaagatgc cccgttgatt atgtcacaag
    13921 attttacagt tttcattaat tctgggtcac atggattcat tatagctgaa ctaaatccat
    13981 tagccattgc cattgctaag aatgcactat ctaaaacagg tctaacatgc ttaggacatc
    14041 cattagatac gttagataat cctcctgtag ttaaaagtcc catctcagtc atcattttta
    14101 tagcttctaa aacttctact tgtttttctt gcataccttt tataactaag cataatgggt
    14161 caaatagtat atcagttggc tccattccta acattaagcc tttttctaat atttcttggc
    14221 agtaagccat acgctcatca ttatctcttg ggataccttc ttttgcacat aaacctataa
    14281 ccattgcttc gtattcagct gctatatcta ttaaatcaaa tcttgagcca gcatcagcag
    14341 agtttattat tggttttgca ttagttctat cataaacttt aagtccagct tcgatagctt
    14401 ttttattagc tgtatctaat gctataggaa cattgttgaa ctcagattga agtaatttga
    14461 ttccccatgt cattatttct tcgccatctc tttcggcagg tcctatatta aaatctatat
    14521 agtgtgctcc tgcttctaat tgctcttttg ctctttttaa gattggagct gggtctcttt
    14581 ctgctaatgc ttttcttatt gaaggtgata tacaatgtat tctctcacct ataatcataa
    14641 atttttccat ttataatgtc cccctttaaa tttaatatta atagttagtt aatattttat
    14701 tagtttgttt ggcatgcttt ttcttggtat ccttttaaga acttaggtaa ttccatagat
    14761 tcctctggtc ctataactac attccatcct ggtaagttat cttctatatc acctttaagt
    14821 actgcaactt ttcctggaat gataaggtct ttacaattag ttacttcttc taccttactt
    14881 tctttaatga aagcagagat tgagtttcca ccaaatttac cagcagccca agatgtaaga
    14941 actgaatatc cacctgcatc tggtattact aaccatactg gaacttttga tctttctata
    15001 tcaccagcaa ctatgaagta agttaatgca aagtcaacag taactaatac tggagaattt
    15061 tcatctgggt tgttgattgg ataaatcttt ggttcaactc tcattggtct ttgtgggtca
    15121 gtgtatatgt tttgtcttag tgcaaataat ggtaatgcct tagcataact aatatcatct
    15181 attactatta tagaaccgta ttttatagtg aatattgatg ataaagcaac ttccatcata
    15241 ggatttgaat tagataatct atttgcaaat actattgaag gatatccaaa tgttctatct
    15301 tgctctttta aagctgttct tctaacttgt atagcattag tgtaagtatc ttttatgttt
    15361 tctcctgtta catctaatac taattcttta tatccagcag cttgaattaa ttcaactgtt
    15421 tcatataatt cttctaagct accagctttt acacctaatg gtaaacttgc tcctttaact
    15481 acttctatca tatctttgta gttctcttta gttgctccat ataccattgg tctagcatct
    15541 tttaatactt caacagcttc tttaactact tgtgcatcat cacaagctaa aatataagct
    15601 acttctaatc cactgccttt tattttatct attaatttta aatacgcatc tttatctcca
    15661 caatactcta aaacagccat ttcaactttc atttgttcac caattcttac atagtcaact
    15721 tttttcatgt tagctatttt agcatctact gcttcatcac tcatacaagt acagaatgaa
    15781 actgcatatc tatttctact tactaatgtt ttttcatgtc taaataatac tgtttctcca
    15841 cctagttcat actcagatgc accagcacca acttttaaag ctttcattaa aggtgcagta
    15901 gcctctgata attttgctat agcgtcatca gacatatgtg ggcatttacc tacttctaca
    15961 gctcctgaag caaccttcat agagaaagcc atacaagttg ggaatccaca atccttacag
    16021 tttttctttg gtgttaattt aaatatatct aaagctttta atgccattat tttttccccc
    16081 taaccttaac aatttaatta agctaatgcg ttaaccaatt cttttatagt ttctattgat
    16141 tttggatgac gaagtataac tgcgtttgaa ccacctacta aaacacttgc tgcagttgaa
    16201 acttccattg ctatagttct ttcttctgga caaccccaat ctggctcatc ttctattgga
    16261 gctatagctt ctttaacatg acctacttca aaagctactg gtgttatgat aggcatttgt
    16321 aatgtcttat cattttgacc aaatgctgca agtctaattc tatccatagt agatgcaacg
    16381 tactcatatc cataaccaac tgctgaacat ccaacattca taactatatt ttcaccttta
    16441 acgcctagtt gagttaacaa tacgtttaat tgtttagcta ggtttatatc aacagaagat
    16501 tcagctccta ctttatgtgc ataagccata ccagctgatg ctcctactcc tttgtagttg
    16561 tcttctactg ctgacataaa taagcagtta tgtccatcca atgtttgagc taatttttca
    16621 aataatttac catctttttc atgatttcct gaaccagcta caactaaagg taattttatt
    16681 gcttcaatta ctgccttagc tacatcagca cattcatcaa ctgacttatc taatccattt
    16741 gggtcagaac catcaaattt taaacaaata aaatctgctt gtgtattagc ttcaacatat
    16801 ttagcccact ctactggaca attagctaca tctttgtata attccttata tgaatcagtc
    16861 cagctttcag gataaacgtc tgatatttgt atacctattt ttggagaatt acctacttct
    16921 ccatcaaaac tataaaaagg taatacattt tccccaccta atttaattgc tttttcccct
    16981 attcctactt caacttctga tatttttcca gaatattttt gagtagacat tttaaatgcc
    17041 atattttttt cccccttaag cctattttaa ttctaattta gcaaaaattt ctttcatagc
    17101 cactttagat tttgaatctt caggtaaatt aactaaagga atacctgtag aatcatattc
    17161 atatatcaat tcatccatag gtacaacacc gattagctct aggttatgct tttcaatttc
    17221 ttctttaaca ccatcattca taaccccatt tggaacttta tttacaatca aaagtattct
    17281 tccaacactt aactttagtt cctcagctaa atctcttatt ctagcaacag cttgtatgct
    17341 acgtcttgag caatcactaa ccaataaaag tgtatcaaca tgtctagtga cttttctact
    17401 tagatgttcc ataccagctt cgttgtccat gactaagtat ttgtaatgac cagatatttt
    17461 gtttacttgt tctctaagta tcccatttac aaaacagtaa catccttcac cttctgacct
    17521 acccattact aataagtcat atccttcgcc ttcttcgatt atagaattta atctaaattg
    17581 aagatattgt gcctttgtca taccaccagg gaatgcattg cctaactttt ctctttgatt
    17641 tacttcttct cttatttctc ctattgttgc ttcaacttcg atacctaata cttcatttat
    17701 gttagcattg gcatcagcat ctacaactaa tactggtccc tttttgtcct taaccaaata
    17761 atcaatcaaa agccctgtga gacttgtttt accagttcca ccttttcctg caacagctat
    17821 attgtatccc attttaagac ttccccctta aatctagcca cagagcctta tgtactctgt
    17881 ctgtctatta taatttaggt acagaatgaa cacattctcc atgtacatca tgaagtgctt
    17941 ccattagccc ttcagataaa cttggatgtg gatgtattgc atcccctact tgttctactg
    18001 ttaatcctag atgaacagca agtgacaact ctgtcaataa atctgtagca tgaggtccta
    18061 ctactgctgc acctattatc ttatcagttt ctttgtcagc tataactttt acaaatcctt
    18121 ggaaatgtcc tatagcttgt gcttttccaa gccctctaaa atcaaattga cctacattgt
    18181 attctacacc ttcagcttca agttgcttct cagtcttacc aacaccagca acttcaggtt
    18241 cagtataaac acatcttgga attgctctat aatctactac cttagtctta cctaaagcat
    18301 tttcaactgc tacgatacct tctttagaag caacatgtgc taggaaagga gtatctatta
    18361 tatcgcctat tgcgtatata ccttctacat tagtttcaag atgttcatta actactactt
    18421 ttcctctttc catttcaatt cctatatctt ctactccaga attgtcaagg ttaggtcttc
    18481 taccaacaca tactaatgca tattgtgctt ctataacctt tccattagaa agagttgcaa
    18541 cagccttacc atcaactact tcacaagttt gtactccaat acctgtaata accttaattt
    18601 tatctttctt aaattgtcta agtagttgtt tagctacatc tttgtcttca tttaatagta
    18661 tttggtcaac catctcaact atagttactt ctgttcctaa ggctctaaag aactgcccta
    18721 tctcacatcc tataactcca ccacctacta ttaacataga ctcaggtatc tcttcaagtc
    18781 caagaacttc atcactagtt attacaactt ttccatcata tgggaacatt cttggtacaa
    18841 ctggaacaga accatttgct agtattatct tatctgcttt tatagtttct actgttccat
    18901 catctttagt tacttctata gtgtttttat caattaattt tccaaaacca ttaactaggt
    18961 ttactcctct tttttcaaat aagaactcta taccacttat taattggttt acaactttat
    19021 tttttctttc cattatggca gtgaagtttg gcttaactgt accatctatt tctataccaa
    19081 aatcctttgc ttcttttaca gtattcaata ctcctgaaga agcaagaagt gctttagttg
    19141 gtatacatcc tgcatttaag caagttcctc caactcttct tttttcaaca actgttacat
    19201 ctgcaccaag catagaagct tttatagctg ctacatatcc tcctggtcca ccacctacta
    19261 ctactatctt catctttaat tcccccaaac ctaatataat ctacaatctt gcaatactat
    19321 aaacctgcgg catccagtat agcaggtata ttctcttctg ccccaattgc cattatatga
    19381 acaccatcac ataaatcttc ttcttttaat tgtttgataa gttcccctgc catttttatt
    19441 ccttcactta cccagttttc ttttccagct gctcttaatc tttcgatttg ttcatctgga
    19501 acaaatattc ctggtacatt tgcagtcata aatttagcca ttcctggtga ttttaaaggt
    19561 actattcctg ctaatacctt gcaatccata tcccttgtta attctctaaa ctttctcatt
    19621 gtatttatgt cataaagtgc ttgtgtttgg aaaaatttag cccctgcttt tattttcttt
    19681 ttcattttta ataattgaac ttctattgga gaatattctg gagttactga tgctcctaga
    19741 taaaaatctg gagaaccttt taacttatta ccagccatgt ccgtacctgc cattaaagtc
    19801 tcagcagttt gaagtatccc cacagaatct aaatcaaaca ctcccttagc ttgtggatgg
    19861 tctcctacgc tagtatggtc tcctgttagt gccaacagat tgtttatccc aaatacacct
    19921 gctgacaaca tttctccttg tatagctatt ctatttctat ctctccctgt tatttgaata
    19981 actggctcta atccagcatc ttttaataat ttacatgtag caagagaagt tgctctcata
    20041 actgccgatt gaaagtctgt tacatttgct gcatgaactc ttccaactaa cggctttgca
    20101 cattctatta aatgtgaaag gtcagtccct tttggaggag ccatttcagt agtaactgca
    20161 aattttccac tttctaatgt ttctcttaat aagctcatct ctaatccccc ttttaacctt
    20221 ctaaaaaaaa cttttatgaa tttgctgctg cttctttctt tttctttgca cttaaacttc
    20281 ttggattatt ttgcttagaa tagtcttttg gtggtcttat ttctgctaag ttatctaatt
    20341 gtcctatagc ttctagtctt tcatatatct ttatccaagc acaatcattc tctgagttta
    20401 cttcacactt accattttta gctccaccac aagccccatt cataagacct ttagcacaca
    20461 tagttactgg acatatccca cctgtccaac caagttcaca gtctccacaa gccttacagg
    20521 cttcttcata ttctccaact ctttgtactt cccctataaa caaagtattg ttggctggat
    20581 aaactggctt atcttttaaa ttcttaacta ttgtctgtgt accatctcca catgctaaag
    20641 ataaaactgc atcagcttcc ttagtctctt cttttagagc ctttaaatcc tttttagact
    20701 ttaaaaggtt acaagcaggg tcaagcatta catatcctaa gatttttttg ccttcacctt
    20761 caagagtttc cttcattttt aaaacttctt cttccccacc acttttacaa gttgctgcac
    20821 attgattaca accaactaaa accagcttgt caaaatcctt taagtaccct agcacttctt
    20881 ctaatggttt attttcagaa attatcatat ctacttcccc cctaaacctt ccacatatat
    20941 atctatttat tgttttgtaa tttacaagcc ttaactacat gttttgcaag tatagatgtt
    21001 gtaactgaac caacccccgc tggaactgga gttatcattg atactttgtc taaaactgca
    21061 tttgtatcaa catctccaca taaatttcct tcttcatcaa catttatacc aacgtctata
    21121 actaccgctc catctttaac aaaactttca tcaaccatct tagctctacc aatagctgct
    21181 attagcacgt ctgcctctgc tgctacacct gatagatttt tagtttttga atgacatatt
    21241 gtaacagttg catgttcact taataaaagc attgatactg gctttccaac taccattgat
    21301 cttccaagaa ctgttacctt acttcctttt aaatcaacat tatagtgttt taatatctca
    21361 actactgctg taggtgtaca tggtggaaat ccacttttat ccccttccat taccttagca
    21421 gagtttattg gactaaaaca atctacgtct ttttccggtg caataacata ttttataact
    21481 tcttcattta agtgttttgg aagaggtcta aaacataata taccattaac atttttgtca
    21541 tcattaactc tctttaatac atcaatatat tcctcttgtg ttatatcctc agctaactct
    21601 aacacttctg ttgttatacc tatgttttga catctcttta gtgctcctct ttcataagat
    21661 aaatcatcac ttcttgcccc aactctaact attgttagtt taggatttat accttcttta
    21721 actagtaaat cgacttcttt tattaattct tcactaatct tatcagctac aggttttcct
    21781 tttataattt gtcctttagt tgacattcct tccatacatt cacaactccc ctacaagcaa
    21841 acttaaatta atttattcta tataccacat attaataaca tagtatctac tttcgtcatt
    21901 taaaaattat tttccaagag ctttttcaac tttggcataa acttcatcac aaatttttac
    21961 accatcttca actaacttat ctacttctgt ttttacctta ttaacatatt ctttatcttg
    22021 tatagaattt atatttataa ctacatttaa ttgtccacca agtatagcag ctcttaaaca
    22081 ctgaactcct acaccaacat cacttatagc aagtcttgaa cctttatcaa ctaaatcttc
    22141 atgtaactta atagcttcat agcaaactct tactatgttt aatggtactt cacaagcaac
    22201 ttttaatgcc ttttccattg tttctgcttt tattcttttt tcttcttctg tttctttagg
    22261 aagcccgtaa gcttttgata atggtaagaa acattctgca tcatcatcta tcatttttaa
    22321 taaatctttt tctaactttc ctgccttact taatatctct tttacacttt cttcatactc
    22381 agcatacttc ttcttcccta ttgtaaggtt acataccata ctacctaaag ccatacctat
    22441 tgcacctact agtgcagcag ctcccccacc acctggtact gcttctttgg aagatagtac
    22501 ttctacaaaa tcaacacaag ttttatctgc tattttcatg ataatcctcc ttgaaaggat
    22561 gtaggaaaat atctcaaatt gttcgagata ttttacgaac tattttaaga tatctcctac
    22621 tctttattta taaattaatt tccttagaat aatccagcta ttactccatt ttcatcaaca
    22681 tctatttttt ctgctgctgg aacttttggt aatcctggca tcttcattat ttctccagta
    22741 agtgcaacta taaatccagc tcctgctgaa attgttgctt gtcttacagt tattctaaat
    22801 cctgttggtc tacctaattt agtttggtca tcagttaaag aatattgagt cttagccata
    22861 catactggaa ctttagtaaa tccaagtttt tctaagttag ctatttcctt atcagcttca
    22921 ggagtataat ctacaccatc tgctccataa atcttagtag ctatagcatt tatcttgtct
    22981 tttatagata aatcatcttc atagcagaat ctaaagttat tttcttcttc atccaataat
    23041 cttaaaactt ctttagctac tgcaattcca ccttctccac cttttgccca tacttcagaa
    23101 agagcaacat taacacctaa ttccttacac ttgtcttcaa caagtttaag ttcagcttca
    23161 gtatcaagtg ggaatctatt gattgcaaca actgctggta atccatatac ttgagttatg
    23221 ttttcaacat gttttaataa gtttggaagt cctttttcaa gagcttctag gttttcgtta
    23281 tttaagtctg ctttagcaac tccaccattg tattttaatg ctcttacagt agcaactatt
    23341 ataacagcat ctggttttaa gtttgccatt ctacatttta tatctaagaa tttttctgct
    23401 ccaaggtcag caccgaaacc agcctcagtt attacataat ctgcaaagtg catagccatt
    23461 cttgtagcta taacagagtt acatccatga gctatatttg cgaatggtcc accgtgtaca
    23521 aatgatggtg ttccttcaag agtttgaact aggtttggtt ttaaagcatc ttttaataat
    23581 gcagccatag caccattagc ttttaattgt ctagctgtta caggttcacc ttcgtagctg
    23641 tatccaacta ttatgtttcc taatctttct tttaaatcag atatatcact tgccaaacag
    23701 aatgctgcca ttatttcaga agcaacagtt atatcgaatc catcttgtct aactgctcca
    23761 tcaccttttt tacccatacc atcaacgata tttctaagtt gtctatcgtt catgtctaca
    23821 catcttctcc aagttatttt cttagggtct attctaagtg catttccttg gtgaatatgg
    23881 ttatcaagca tagcagctaa aagattgtta gcagctccta tagcgtgaaa atctcctgtg
    23941 aagtgtaagt ttatatcttc cataggaaca acttgtgcat atcctccacc agctgctcca
    24001 cctttaacac caaatactgg tcctagagat ggttctctta aagcaactaa tacatttttg
    24061 tctagtttag caaatgcatc agcaacacct atagtagtag tagtttttcc ttcacctgct
    24121 ggagttgggt ttatagctgt agttagtatt aacttagctt ttttaccagt ctctcttttt
    24181 agtagattgt agtcaacttt tgctttgtat ttaccataaa gttctacatc atcttcacct
    24241 agtcctaatt tttttgcaac ctctctaata tcttgaggct tagcttcttg agctatttca
    24301 atgtcagatt taaatcccat aactgaatct cctcccacaa cctaaataga tttaaaatat
    24361 aatttttgta aaaattgtat taatatgtaa aaattatatt ttataagata attatacatg
    24421 taaaattact taaaaactag ctactaagct attaaaatat tcaaacttaa tagctagtta
    24481 tttatctaat tacttactta ttacctatct attgcctatg tctaaccttt aaccaactta
    24541 aaatcccaaa tcaaaatttc tatctatttt atttatcctt aagtgacatt aatttatctt
    24601 ttattgcttt aatctgttct ttcgttgttt cactagaatc atatggagat tgattagctc
    24661 tatcagaatc aataacatct tggttataat aaataaatcc taaagattct ccatcttcta
    24721 agttttggat tataaattct tcatcttcct tatttctaat tttatttcct actacaaata
    24781 ctttatttac acctatatca tgtccaagtt tcttaacttt tctataagtc tgtagacttc
    24841 tctctcctgg ttctacaact actataaaag catctacacc ttgggctgtt cctcttccta
    24901 agtgttcaat tcccgcttcc atatccataa ccactacatc tttattttgt agtattaaat
    24961 gtgagcagag tctcttcaaa agaacatgct ctggacatac acatccagta ccaccagaat
    25021 ctacggttcc caatgtaagt aatcttactc cattgtattc tttacagaaa ttttcaggga
    25081 tatcgtctac tttaggattc atcttaaaca tttttccaaa tgaacctaca gaagcagctg
    25141 ttctatctga aactaatttt ttcatttctg atataggtac tattgattca tatacttctt
    25201 ttggaaaacc tagtgctaaa gctaaattag catcagggtc ggcatcaaca gcaacaactc
    25261 tgtatccgtc ttcagcaaac atcctagaga gcattgaaga aaaagtcgtt ttacctactc
    25321 cacctttacc tgttattgct atcttcataa tccctaccac cttttattac tatttgcttt
    25381 ttagtagtta attttacagt taaatatttt ttatactcta tatttttata cactaaaaat
    25441 aaactacagc tagtccttac aggaaccaat taaatttacc ctactagctt ttcaaagcag
    25501 catgtatagt tttattattc tttaatattg tttatatttt atttattcag tatattcatt
    25561 atattttgat gttctttact ttaatatttg actttattca actttattca accagaaact
    25621 tcttataaaa acgtttattc tataacttca ctatttttta agcttcagcc ttttctgtca
    25681 ttttttcttc aactaatgct tcaaagtgaa cacgtttttt ctctatatct tctattattg
    25741 tagctgctaa ctcttttgga tttgtattta cagtaaatgt agctccaact ttatctttta
    25801 attcactagt taatatatcc actgctcttg aagaaccagc tattggaggc attattccta
    25861 aataagtgtc tataccagaa gctacaacat agcatcctat agcaactgct ttttctgaca
    25921 tccactctgg agctatacct acaactggaa ggtcacacat atccatatct aagtaattag
    25981 ctgctgctcc aactaaatct agtatacggc ttatatcaac acaagaaccc atatgtagta
    26041 ctggtgggat tcctactaat ttacagacag ttgcaagacc tttacctgca tatttttcag
    26101 ccgcatcaga ttccattaaa ccaaacttag ctgctgctga agctccacaa cctgtagtaa
    26161 ctactattat gtcgtttttg attaattctt tcattattgt tacgtgagcc tcatttgaaa
    26221 ctcctttagc attgttacat ccaactacac cagctgctcc tcttaatact ccagctttta
    26281 gacagtctgt taatggctta actgttcctg ctgggtctat atgagagttt acaactctgt
    26341 ctaattgatt tactactgct tctacactat acccaactgt tgcccctgat tttaattcag
    26401 gtataagtac ttttgattta tctctatttt taaagtttaa aatagcttct ttaactattg
    26461 ctttagcatc atcaagtgct tgttcttctc taaattcaat ataagttgaa cctgttatct
    26521 tagcttttgg agaagttgtt acaaatttag tatggtaaca atctgcaact ctagctaatg
    26581 cagggaatat acattgaacg tcaactatta cagcttcaac tgctccagtt attattgcaa
    26641 gttcttgttg atggaagtct cctgctattt ttacaccatg tctcattgta atctcattac
    26701 ctgtacaaca catacctgct aagtttattc catcagcacc aacttcttta gctaaagcta
    26761 ctaagtctgg ctcttctgat gctaaaacta tcatttctga taatgctggc tcatgtccat
    26821 gtaatattat gttaacttta ttttcttcta atactgctaa attcgcctct gttcttcttg
    26881 gcactggagt tccaaataag atatcgctta gacgagtacc tatcatagaa ccaccccaac
    26941 catcagccat agaagttcta agactcatgt gtattaaact atctatatca ccagtacatc
    27001 ctatatgagt agagtgcatt attgttgcaa tttctctatc tatagctctt ggctctatag
    27061 cgtattcttt ccatattttt tgtcttggtt ctggtgcatt ttttataaat cttaatgtcc
    27121 caaaaggttt tccaaattcc attaatgcaa tttctgccac ttcatgtgct acatcataga
    27181 tatctctacc ttctgtctct acatcccatt ctttagctaa agtaattaac ttagcttcgt
    27241 ctcttatttt atagtttcca tttggatcag ctaatgctaa tgtatgtgct atatctctag
    27301 catggtctga atgggcagct gtcccaccag caaccattct tgcaaagttt ctacctacta
    27361 tagtatgctc atctgctcca cataaccccc ttggagtctt agggcttatt ctacatggtc
    27421 ccattccaca tattctacag caaacacctt gaagaccaaa accacattgt gccttaaagc
    27481 cttcttttct atcatacatt gtttctacgc cttcttctct agctttagct atcatcttct
    27541 ggctgtttaa atcaattgtt aacatttttt catccatatt cctatcccct ttttttgatt
    27601 tttaaaatcc cccaggtgac tgacttcatg tcataaaata ataaaataaa tgctttctaa
    27661 gttgattata tactaatttt cagtgctttg ctttagattt aaatattttt ttaacaaaat
    27721 tagataatac ataaaaattt tattttctac acttaattta agttgatttt atcgcttgtt
    27781 tttggctatt tgttaatatc taaacctact tttttgatac ttcagtttaa ttaaattatg
    27841 ttttgatttt tttaaatcaa atttgaaatt tttctagtca tttttatatt attgcagatt
    27901 gtttgtgtat ttattatctt tcactactgt ttggacttat tacagatttt tactccctaa
    27961 acttaattag taaaaacttc ttagaatagc attttttgga ctaatttttt tgaatttttt
    28021 tatttttcct actttaatat taattatagc ttcaaattga acatttgtat atattcattt
    28081 gaagctataa ttatttttta tataaaactt ttttaataca gcattaattt agacattttg
    28141 actatttttt ctactttaat tcatttattt caagcgattt atcctatttc atatttttag
    28201 ctacaatagc cccatatgca atagaatata atttagattc atgtgaatca gctcttgaca
    28261 ctaaaactat aggagctttt gcacccataa ttatacctgc tgatttagag tctgccatat
    28321 atgtaaaggc tttccctatt ccattaccaa cctctattgt tggaaccaat atgatatcta
    28381 catctccaga tacttcactt ttaaaccctt ttatctcact agcttctttt gaaacagcta
    28441 aatcaaaagc cagaggacct tctacaatta cattttcacc aaattctcct tctttacaag
    28501 cttttgctaa catatctgca tctacagttg cttgcatctt aggatttact ttctctttag
    28561 ctgccaaaca tgcaactttt acagtttcca ttcctaaagc ttttgcagct tttatagaat
    28621 tttttaatat ctttgccttt tgttcataat caggagctat attcatacct ccatctgtag
    28681 ttataagtaa tttatggtat ttttctaact catataccat aacatgactt aataaactat
    28741 cagttctaag accatagtct ttatttaaca cttcttttaa tatgacagat gtatctaata
    28801 atcctttcat tacaaagtca gcttctttac ttgatactaa tttaactgct atttctgcac
    28861 actctttgat atcatcctta tccactattt tgataccact taaatcaaat cctatttctt
    28921 ttgatatttc tcttattttg ccttcatttc caaccaatat aggagtaatt atatttcttt
    28981 ctacagcatc ctttattgcc attaaaactt ctttatcatg agctgctgct acagagagta
    29041 caattttctc tcctccctta agttgttcta aaatatcatc taattttttt atcaaaagta
    29101 acccctccct tatatatatt taactaaata ataccatatt tgctatacct tttctcaatt
    29161 tattttttat gttataaaga atttatataa ctaaaagaca gtttaatatt ttattctggg
    29221 atttcatcaa taactcttaa aagctttgca ggaacaccac ctacaatagt attttctgca
    29281 acattttttg ttacaactgc tccagcagcg ataattacat tatctccaat agtaactcct
    29341 ggaactatag ttacattagc accaatccat acattttttc ctattgtgat tggagaagga
    29401 taagttgtac cacgtttctc tggtgcaata ccatggttaa gtgtagccaa taccacatta
    29461 tgcccaattt gcgtattatc tccaatatca atccccccct ggtcttgaaa atggcaactg
    29521 ctatttataa aaacattttt tcctattgtg atgttttttc cacaatctgt ataaaatggt
    29581 ggaaacataa aaaatgattc atctaccttt tttccaatta actcagaaaa taaactacgg
    29641 acttcttctg gttcatgata agaagaattt aacttacttg tgattttcat tgcttcatta
    29701 ctcaacttaa ccataaattc atgtatttct gatttactag aaacaggttt tctttgattg
    29761 atatgtgtta aaaattcatt gatattcatt ttatacatcc tttcaatatg tgctttaatt
    29821 tatttgtaca ttctaaataa tattttaata aaaattaatt taatttattt ttattaaaat
    29881 atcattgctt cttttagtgt aatatacaat tatatctata tcaaatatct atattaaata
    29941 cttaactata cttaaaaagc atataaattg atataattat ataatagata agttaatatt
    30001 gaggagggat aattatggaa ataagagtat taagatattt tttagcaatt gcaagagaag
    30061 aaagtatatc tggtgcggca acctttttac atataacaca accaactctt tctagacaaa
    30121 ttatggaact tgaagaagag ttgggagtta aattattggt tcgaggaaat agaaaaatca
    30181 ccttaacaaa tgaaggtagg cttttacata aaagagcaga agaaattata tcattagttg
    30241 ataaggttga attagaattt gacaatccca atgagattat tagcggtgat atatatatag
    30301 gaagtggtga aactgaagct atgcgactta tagctagaac tgcccatcat tttcatcaaa
    30361 aataccccaa tgtaagatat caccttttta gtgggaatgc tgatgatgtg actgaaaaat
    30421 tagacaaggg actgcttgat tttggtattt taattgaacc tgcaaatata cagaagtatg
    30481 attatgcaca aattcctgct actgatgtat gggggttact aatgcgtaaa gatagtgccc
    30541 ttgcatcaca aagcacaata aatccaaaag atttatgcaa tatacctctt attatttcaa
    30601 gacagacact tgttgataaa gcaatttcta aatggcttca agacagtaat gaaaaactaa
    30661 acattattgc aacatacaat ttagtttata acgcttctct tttggtagaa gaaggtctgg
    30721 gctacgctct gtgtttagat agactcgtca atactacaga aaacagtaat ctttgtttta
    30781 gacctctatc tcctccttta gaagctaaat taaatattat atggaaaaaa catcaaatat
    30841 tttcaaaggc agcagaaaaa tttttacttc aaatacaaaa agatttttct tctatcatgt
    30901 tggatacatc taagttggat acacctaaag aaaaataaat cataataaaa agtgagagtg
    30961 tctcaaaatg aactttgcac tgtataaggc actctttttt atacaaagta aaatatatgt
    31021 tcatcatttc agcactaaaa aagaggctta tctctatttt gagatagccc cacttaacca
    31081 ataaaattaa atacaactta acaatatcct tacttattta ttccaagttc tcttagacaa
    31141 gcaatagtaa tttcatcttg tggatttaat tcataagctc tttctatata gtaagttgca
    31201 tcatcaatat caccattatt taaataagcc attcctaaat tgcaaagtat ttctgggtct
    31261 tcctttatct tagccgcttg ttcaaaatac tctattgctt tatctaaatt attcttataa
    31321 gcctcacaaa gccccaattc cactatagta tccacttgat taggctttat tatcaatatc
    31381 ttctcaagat acatcttagc ctgctctatt tcgcccatac ccttatatcc aagtgctatc
    31441 ataaacagca agttccacca atccatatgt tcttcttcta atggaagtaa cttctctaat
    31501 ccttccttaa acttaccttg gaatacaagt gtatatcctt cttcatattg aactttaaag
    31561 tccatcttgc ccagattctc ttgtacctca gctactaaat cagcatctaa ccctagcctt
    31621 aacgcttctt cccaagtcag cttagtcttt aaatattgac cttgattata ataatggtag
    31681 cctaagtgat aataacctaa tgcaaaattc tcatcaacat caattacctt ttcaagctta
    31741 gccatggctt caagtaagaa tgcattcatt gccttatcat ccatatcttt ttgatactgt
    31801 ttagctatct cttggcaaac aatcgcatag ttatacattg catctaaatc atctggatac
    31861 attgttataa atgcttttat atatattaaa gaatctgtat gttcaccaat ctcaaagtat
    31921 tttctcgaca tatatcccat atacgatttc aaatccaaat caatatcttt tgataaagca
    31981 tttaaaaact tctcatactc tgcattgttt ttaaacttac tatctattcc aattatatat
    32041 atcattgcat ctgctataga cattgaattt aacttatctt gagcagtctt ttcttttatc
    32101 ccttttacta atacctcatt ttttataggt acatctaatc caccttttgg cacttcatag
    32161 ctttttaatt taaactctcc accatgtttt atagttataa aagctaattc ttctgttttt
    32221 tttagcaaat atttttctat cttaaaatcc attttatcct cctaaatact taaatctcta
    32281 ctttatgagt tttagatttc tcaaatattt ttttatcatc atactcaaat aagactacat
    32341 tttctacttc tcttatttct gtatttatat cattttttaa atccacaaca ttaaatctaa
    32401 atggctcaaa atgaacatat tttattatat gtttagctgg tttatcaaca aaactaggta
    32461 aatatgttga taagttatca ttatcttgta aaaatacatg gcatctattc ttaaaatcat
    32521 ccacatctag cttattaaaa aactgtggta tatttgatgt tttacccagt gatatatagt
    32581 catattttaa tatatcgttg aatacatcat tatctatgtt gattttttca ttatagaaat
    32641 ccatcaatat tttatataac tgattctttc cttgtgccaa atcaaagtag ccattttcat
    32701 caaaatactt ggcaaaaacc tcaaagaatt taaaagggct ttttttataa aatctgccta
    32761 taatatatct catagataac acaaagtttt tagaattgta gtatctctcc agtatatctt
    32821 ctatatcctt tagcttcaaa gtttcttcat agcttatata attattgtat aaaaactcat
    32881 atggagcata atccttatat ttaaaaccat gctcatctgc aacttttctc atacctgtcc
    32941 ctttaatcat ctttaaaaag cctaattgca aatgctctat acctagatta aatacatcat
    33001 taaaagaatt ttcaaagctc ttataatctt cataaggcag tcctgcaatt aagtctaagt
    33061 gctgatgtat atttctataa gatgctacag tttgtacaac atgagacaac tttttaaaat
    33121 catcccttct acctactgca tcaagtgttt tttcattcgt tgactgaaca cctatttcaa
    33181 attgaaaaag accttcttta caatcagcca aaaactcaag catcttatca tctatcaaat
    33241 acgctgtaac ttcaaaatga taagttgtat atccattatc atttttcata aggaaatcca
    33301 tgatttccat agccactttt ctatttgcat tgaatgtcct atctataaac tttatttgtg
    33361 atactcttgc atctattaaa gcttttaaat cactctttat cctgtctaaa ctaaaatacc
    33421 ttagacctgg aatagtagat gataaacagt attggcagtt aaaagggcaa cctcttgatg
    33481 attcataata cactattcta ttttcatatt cttttggatt caagttttca taaggactag
    33541 gaatctcatc caaattttga agtaattcca taggcttatt cactattatc tcattgcctt
    33601 gtctatatac aagtccattt acatcattta tttccatctt tccttgcaag tgaagtacta
    33661 agtctctaaa aattaattct ccctctccat aaagtatata atccacaaaa tcatactttt
    33721 tcattgcctc ataactatca taactcactt ccggtccgcc caaagctatt ttagtattag
    33781 gtctaatttt ttttagatta tcacaaagtt taactatatc tcccacattc cagatatatg
    33841 ttgagaataa tattatatca tattcatttt tatatatatc ctttaaaata tagtccaaat
    33901 cgttgtttat cgtgtattct ttcatattaa cttctatcaa atctcttaca aattctttta
    33961 gatatcttat agccaaattt gtatgaataa acttggaatt taatgttgtt aataaaattt
    34021 tcaaatattt ctcacctctt tttaagatta tactgtcttt ttgtacccct tataggttaa
    34081 aatagtatat gtaaagtcta aataatatct atataattaa tttattaatt tcaagtaatg
    34141 tgaggaaagg aagtttttta tggaattaat tgaaaaagta tctatcaact ctttatcaaa
    34201 aagagattta ctacttatta taaagtctct tgaatacact aatgaaaata ctcaaattag
    34261 tgatttcatt gaccttagga ataatatagt aaaggaatta tgttttttaa ctgacactaa
    34321 ggaaaaagat tttttagatt atctagaaaa aaatagcaca ctctaataac ctaaaccttt
    34381 ttaaacttta atatataaat acctgattta gaatttattt ttatgcttaa tatattatca
    34441 ttaaaggttt tcgacataac tgttatttga ttaaatatag ggtctattat agtattacct
    34501 tcttcaagtc ttataagact tcttgggtct atgcttttat tatatgcctt aactccaatt
    34561 ttatattttc tcaatttaac aacatattcg gacatagaaa aaataaaatc tatattatct
    34621 tcatttaatt tactatcttc acccaaaatg gataatatag atttttttat gctattaatt
    34681 ccttcatttt ctacaattct atcttccata tatacatcat atagaacatt taaaacattt
    34741 aataaaatac tttcttcatc ataatcttca gatttcaaat caatgtactt atcaattaat
    34801 tgtattgctt taatccttgc catctgaaac tttacaacat ctgatttgtc taattctata
    34861 gaaaatccta ttatcttgtc tttaatactc tgtagtaatt cgacatactc tattttacta
    34921 ttatttataa gattgtgcat aatggaatta tacaccacag aacctaataa ataatcaaat
    34981 aaaagttctt cttttttaaa ataaataaca tactttttta ttagtttaat aacctcatct
    35041 tcactttttt gaatattagt atttgataaa attagtggta ataaaatatt taaaaaattg
    35101 atatttccta ttttttctcc ataaaatcta tagtaaataa gttcatctct cagtctattt
    35161 tcgtctatat acacacatct ttgtgtttta tcatctatta aggattttgt atttattata
    35221 gttaaatctt taagagccct acttgcacta ctaaaagaag atgtgtagac tttttttagt
    35281 aattcgtcat tcataaatat atcctttctg aaaaattgaa tgtaaataaa ttgttattaa
    35341 aaaaaaaaga gacagcccgt ttaagaagct gtcatatata gggggatagt agtgtacaaa
    35401 aatcattgta atctactatc tatatattat tatacgatac tactttttta tatgtaaagc
    35461 atcagttttc gacatcccta tattttacag ttatacaaat tgcgtactaa gttcaatttt
    35521 attcttttta tttcaacatt ttatcatgct acattctttc aggagcgtgc attcccaaca
    35581 atttcagaga attttctaaa gtttgttttg ttgctaaaac taatgcaagt cttgcttttc
    35641 ttatttccaa attttctact aaaattggat tgtcatgata aaatttatta aacgcttgag
    35701 ctaaatctag cataaatcta gtcactatat gtggctcatt tttctttaga gcaagtaaaa
    35761 tattcttatt gaatgattct ataactctaa gaacttctgc actatcttca tcagctaata
    35821 gactataatc tatatcagaa gtcacttcta cttctgcttt tcttaaaact gcacagcatc
    35881 tagcatgtgt atactgaaca tatggtccag tctcaccatc aaaactaagc gttctttccc
    35941 aagagaaggt atagtcttta attctactat tagataactc ttggaatact actgccccaa
    36001 cacctacttg cttagatatt tcatccacat ttttagcatt tgggttttta gctagtataa
    36061 tttccttagt tttatctata gcttgattta aagcatcttc taaaaatact actcttcctt
    36121 ttctagtcga catagtacct tcttcaagtg caaccatacc aaatccaaca tgaattaaat
    36181 cttttgccca atcatatccc ataagctcaa ttaccttaaa ccattgttgg aaatgtaagt
    36241 tttgttgaga accaactaca tatatacact tgtcaaaatc ataagtttct tttctatata
    36301 ttgcagcagc taaatctctt gtcatatata acgttgaacc atcattctta gtaataagtg
    36361 ctggtggcat tttatactct tctaaatcaa caattcttgc tcctttagac tcttttaaaa
    36421 gatttttctc ttctaatagt tcgataaccc tgttcatttt atcagaataa aaactctctc
    36481 ctgctaaaga atcaaattct atatctaaaa gtttataaac tctgttaaac tcttttaaac
    36541 tctcatttct aaaccattgc cataattctt gtgcttcttc gtcaccattt tctaatttat
    36601 taaaccatgc tcttgcttca tcttccattt ctgggtgttg ttcagcttca tcatggaatc
    36661 ttacgtataa ttttagcaat tctggaattg gattagactc tactacttct ttttctcccc
    36721 actttttaaa ggccactatt aacttaccaa actgagttcc atagtcacct aaatgattta
    36781 ttcttatagt cttgtatcct tgcgaatcat atatcttata taaagcatta cctataactg
    36841 tagttcttat gtgccctata tggaaaggtt ttgctatatt tggagaagaa tattcaacta
    36901 ttacagtttt atcttttcca aaatcactat gtccgtaatt ttctttttca tctaaaactt
    36961 ttttaattac agtttctgct aattgagatt tatttacaaa gaaatttaca tatccgccta
    37021 attgtattac ttttgttatc tctccacttg gttctatact ttccgccaat tcacttgcta
    37081 tcatgtttgg agcttttcta aacactttgg ctaatttaaa gcatggaaaa gcatagtccc
    37141 ccatatcctt atttggtgga acttctataa gtgcctctat ctcttctttc gataaatctt
    37201 ctattttttc tttaaggcaa tttgatattg ccaccttaaa atcttgcatt ctgttgctcc
    37261 tctctacatt gttcctggtt tatattgtgc ttatctcttt atgttaaatc aaatttcgtg
    37321 ttttttcaag tatttcatta cagtgtaaaa cactttagta attatgtttt ctgtatatat
    37381 taaaaatact ataattgcag tttttaatca cactattatt gattttttat aagacttttt
    37441 tctattttat tgtttcttgt actatccagt tgactttata tacttttttt aatcttttat
    37501 ctattttcat acgtcaatgc taattttata tacttttctt ttaatctttt attaattttc
    37561 atacatcaat gttaacttta tatacgtttt tttaatcttt tatctatttt catacatcaa
    37621 tgtcagcttt atatactttt tttactcttt tatctatttt ctataaatca atattagctt
    37681 tatatacttt ttttactctt ttatctattt tctataaatc tttttcactc ttaactttta
    37741 ttccttcttc ttctaatagc cttgctgtta tacctttccc tggtatcttt ttacctgaaa
    37801 aactaccatc atatattaga ttacatccac aagaggggct accctctttt agtattgctt
    37861 cgctacatac atataaattt gctattttta gagtttcatt agcccctctt acaaagtttt
    37921 ctgttacatc cttattatct ttagtaatta cttttccatc aactatctca gcaggtggtc
    37981 ttggtgtaga tagaccacct aactgctctg ggcatacaag tgtaaattct ttgcctttta
    38041 aatattcctt gactttttca ttatcattat tatcgccact atatttacaa tttattccta
    38101 ttaaacatgc agataccaat atcataaaaa tgtgctccta tacaaattat atatattctc
    38161 atacaaatta ctaatgtatt cttatacaaa ttattttaat ttaacaattg ttactcctgc
    38221 tccaccttct ccatattgac cacctctttg tgattttaca tgtctatttc ttcttaaaat
    38281 gtcttgtagc cctgctttta atactcctgt acctattcca tgtattactg ttacagactc
    38341 taaacctgcc acatatgcat catcaagata cttttctact tccattatag cttcttctag
    38401 gtttaaccct ctaaggtcaa cttcattttt tacactacca gattttgacc ttataatgtt
    38461 tctcgttgat ttagttacat ttgttgaaac atctttgcgt gttttttgta aagatttaaa
    38521 aggtagagtc atcttcatta taccaatttg cactactgct tcttttctct ttttatctac
    38581 tgaaactaca ctaccatttt gatttaaagt tattacctta acttcttcac ctggctttaa
    38641 gtcttttatc tccttatttg atacctttgg aactatcata ctttttactg ttggctgaag
    38701 acttcccata gagctagtca attcttttct aagttcttct atcttacgat tcttttcttt
    38761 tgaagctctt tcttgttcaa gacttctaag ttcttttatt ataatatcta cttcttcttt
    38821 agcttgtctt ataattgaaa atgcttctga tttagctttt tcaatcatct tatctctttg
    38881 actgaccaat ttttcaagtt tttcgtcata ctctactttc agtttttcaa tctcttcttt
    38941 taatctttca gcttcttctc tatcttctac tgctttgatt ctattctttt caacattctg
    39001 taacacatct tctaaagcta tgttttctgt atttatatat tctttagcac gtgaaatcac
    39061 ataatcactt agcccaagtt ttctagaaat ctcaaacgca tttgattttc cgggaacacc
    39121 tataagtaac ttatatgttg ggcttaaagt ttctatatca aattcaactg ctgcattttc
    39181 tacacctgat ttagtaagag cataattttt aagctcacta tagtgagtgg tagctataca
    39241 tttagctcct acagaattta tatcttctaa tactgctatt gcaagggcag ctccttcaac
    39301 agggtctgtt cccgctccta actcgtcaaa tataactaaa gagtctgctg ttacgttttg
    39361 taatatagat actatattcg tcatatgaga tgaaaatgtt gatagactct gttcaatact
    39421 ctgctcatct cctatatctg caaatacatt atcatataca cacatactac ttccataatc
    39481 agcaggtata tgaagtccac tttgtgtcat aagtgcaaat agacctacag tttttattgt
    39541 aacagtctta ccacctgtgt ttggacctgt tatcaccaat gtatggaagt ctcttcctaa
    39601 ataaattgta tttgctacta ccttcttttt atccagcagt ggatgtcttc cattttttat
    39661 gtttaaatat ttatcctcat ttaaagttgg ttctattcct ctcatctgta tagaaagttt
    39721 tccctttgaa aatgcaaagt ccaatcttcc aagtatctct tgattagaaa ttaagtcttc
    39781 actaacttct ccaaccatag cagaaagctc tgataaaatt ctttctattt cctcttgctc
    39841 tcctagtttc aactgcctca attcattgtt catctctact atagtcattg gctctataaa
    39901 aagtgtcgca ccagaagatg actggtcatg cactatacca gctacttgcg acctatattc
    39961 agattttaca ggtactacaa atctatcacc tcttagagaa attattgcat cttgtaagta
    40021 cttttgataa gtagttgaac ttattattga atttaatttt gacctaatgg attgattttt
    40081 ttgggctatt cttcttctga tatctctaag tgtacttgaa gcattgtcag atatttctac
    40141 ttcactaatt atagcattgt atatctggtc ttctatgtcc ttgtatacat acagagaatt
    40201 tgatagagat tgtattattg gataattaaa atcttcttca tcactactag ataagctatt
    40261 tcttaatctt ctagccactc ttaaggtatc tgctatcata atcagacttc ctgggtcaag
    40321 agaagcacct atataagctc tctttacctt atcttctata tcatgaatac cttccaaccc
    40381 tactgaacct ctctttataa taattgattg agcttcacta gtttcttcta gcatagattt
    40441 cacttctaca aaatcagtat ttggtactaa attttctata tattttagac ctaaagaaga
    40501 tgaggctttc ttctttaaca aatctattat tttattgtat tctagaactc ttaacgattt
    40561 ctcattcatt ttctatattc accatcctcg atattttaac ttttttattt aattttaaaa
    40621 atgtatgttt tcaagcattt ccaataaaaa ctgcaagttt tatttctttt tcatttctaa
    40681 aattattata ttttttctct tttagatata ttttacacat attttgtatt atactatgta
    40741 tgtagctatc tatatattat acataaacat gctttatttg taatataatc tgttaaaaaa
    40801 tcttctttaa tatttttaaa ggagtttatt ttagcttata aaaaaaaata gctacttata
    40861 tagataaatt aaggaggaac atgagtcatg ggaaacgtat tagaaggatt aaaaccagaa
    40921 agtgttttta aaaactttga gaaaatatct caaattccta gaggttctgg aaatgaaaaa
    40981 ggcataagtg attttttact tagctttggt aaaaatttag gtcttgaaac tattcaggat
    41041 gaaagcttaa acataataat aagaaagcct gctacaaaag gatatgaaaa ttgtccaggt
    41101 gtagttcttc aaggacatat ggatatggtt tgcgaaaaag aaaagaatgt tgaacatgat
    41161 tttcttaaag accctataaa attaagaatt gatggtgaca tgatatatgc tactggaact
    41221 actctaggag ctgacaatgg tatagctgtt gctatgggta tggctatatt agaagataat
    41281 actttagagc atcctgcact agaagtttta gttactgtta atgaagaaga tggtatgaat
    41341 ggtgcagatg cactagaccc tagcttaatt aaaggtcaat atatcctaaa tatggactcc
    41401 gaggaggaag gatacttatt agttagctgt gctggtggta aaacttgtgt tgttagttta
    41461 cctgttgaat acaaggaagt taaaggtgac aaacaaggat tattagttga ggttactgga
    41521 ctattaggtg gacactctgg tatggaaatt gttttacaaa gagcaaatgc taacaaagca
    41581 atagctagag tattaagtgt attgaatgtt gattatgaat tagcttctgt tgatggtggt
    41641 actaagcaca atgctatacc tcgtgaagct aaatgtgtaa tagctgttaa taaagctgat
    41701 gttgaatctg ctaaaaaaca aataaatgac attttaactg catttaaaca tgagtttact
    41761 acttctgacc ctggcatgac ttactcagta gctgaaactt ctgtagacaa agttcttact
    41821 aaagattgta aagaaaaagt tgttcaaatg tcttgcttaa ctcctcatgg agttcaatca
    41881 gtaagtcttg atatagaagg attagttgaa agttctacta acttcgctat aattgaaact
    41941 aaagaatcta ctattgaatt tttaacttct gttagaagtt ctgttatgtc tattagagat
    42001 gaaatcgctg atagaataag acttttggct caagctttag gtgctaatta cgaccttata
    42061 gctcaatacc cagcttggga atttaaaaaa ggctcaaaat tagaaaaaat atgcagtgaa
    42121 acttatgaga aattaactgg taaagttcct actgttatgg ctctacatgc tggtcttgag
    42181 tgtggattat tacttgataa acttcctcat gctgaagcta tctctatagg accagatatg
    42241 tttgatgttc atactccaaa tgaacatgtt agtatccctt ctgtagctaa tgtttgggat
    42301 tatgttattg aaatattaaa atctatgaac caatattaaa tttaagtttt gattctaagt
    42361 agattttaat tttaagtaat ctatataaaa ttaactctgt aaattaatac tactgttagt
    42421 ataatattta ctttcaatat taacttatag aaataaagcc taatgataga taataacatg
    42481 tatcattagg ctttattgta atttaaatat tattaagctt tattctgttt taaaattaat
    42541 tcttatacta ttttaaatct atttcttcct tgtctcttag aatcataaag agcagtatct
    42601 gctatttgat acagttcatc atactcttga ccaggctttg ctataactac ccccatacta
    42661 atagataaac tttctccatt tggcaactta atgagatgaa tatgattgta tacatcatta
    42721 catttattaa taagttcttc cttatttatt atatcattga tataaacact aaattcatct
    42781 ccacccaaac gacctactat atcatttttt gcaaaacttt catcaagtac ctttgcaaca
    42841 tcaataagta ctttatctcc taacaagtga ccataagtat cattaatcga cttaaaataa
    42901 tcaatatcta taatcaataa agcaccattt ttttcagttg ataaattcaa caaattttgt
    42961 gcagttaatt ttctaatata tgctgcatta taaatattgg ttaaactatc cctttgagcc
    43021 ttatctaaca atctattctt ttcctctttt tcagaatcaa tattacatat tttcccgatt
    43081 acatacattg gtatttttct atcattataa attatttttg aagtaactct aatccaatga
    43141 aaagtatcat caagcataat agaatacatt tcagttgttc cattttcttg tgattttatt
    43201 acatcaaaaa actttttatt atattctttc cttattttat ctctttcatt ttctatattg
    43261 ccattttcac tttccttatc atttttatcc aaaccataat aagtctcaat acccttttct
    43321 ggcatagaaa ttatcaattt atcttgctta taatcatact caaaaaagta ttcacttgtc
    43381 atttcataca actgagtatg ttttttgagt tccaaagata tcttctcgtt catacttgta
    43441 cgagtatgta aaccaaatgc cataaatcca ataatacaaa ttaataaact ggctacaata
    43501 aaaatagtct caattggatt ctgttgcata atataaagca acgagaaatc ttgtctatat
    43561 gtagtatttt ggtagacaat tgattgcatt ttttctgatg aaatacttat aatagcttta
    43621 ttaaatatat tcaataattc ttgtttatct tttttaatta taccaaaaca aatactcttt
    43681 gctttatagg tttgaggaac tagacgcaca tttttatact taggttgatt catatagtac
    43741 tgcatagaat aaccttcgcc ataagtgtaa tctgcatagc ctttattaac agcttctata
    43801 cactcttcta aattatcata ccaaactata ttctcttcaa actgaccata ataattaata
    43861 cctttggcta gagctacacg ttttccatct aaactattct catttaattt attattaatc
    43921 atcatagtat attgagtaga aagaaaaggc cgagacatag ctatattatg ttcacgagag
    43981 tattcataat cataaatcat tcctgctaca atatcaattt cacgcttttt agtcatgttt
    44041 tctaaatctt cttgagattt tacaggcaca agttcaaatt ctaagcctgt tttatcacat
    44101 atatgattta acaaatcaat tgatatacct cttaattcac ctgtatcttt atctatattt
    44161 tgaaatggtg gtttgtttaa aagtactcct acttttaatt tttctgtctc tttaatgtaa
    44221 ttagtttctt catcagataa aaacatttga ttattctctg gtgtaaagta tttttcatat
    44281 aaagtagttg aaaaatatgg gtctttctgt tcaatattat taattgctga gttaagcttt
    44341 tgagttaaat ttgtactacc tttaggagta atgaaataaa atggttttga tgaaaatttt
    44401 gctacagtac gtacaccttc tattgggttc atactcacat taagaagtac atctgcttta
    44461 ccactcttta gtgcttctag ttgctcatca tttgttttac aatataccaa ttcaggtgac
    44521 ataagattca ttttacaata ctcatctaat tctttaagtc tagtatcaga attttctatt
    44581 acagcaatac ggattttttt cacatcttgt gaactcatta tgttatcaat atctgtattt
    44641 tcatataatg cttgtagtac tgtatttgct actccataat tattccctgc ataattatat
    44701 attttttgca ttggttcatt gtataacata cctcccatta tatcaatttc accattttct
    44761 aacatcttca ttaattttgt aatagattca tttatctctc caggaacttg aacaaattca
    44821 taattccatc cagtatactg tgcaatttct agtaaatatt catatgtata cccaaaatag
    44881 ttaccttgct catctatttc agttaaacct ttttgaatag gataagcaac acgaatggta
    44941 cgattatcat tacctatagc gttgatacca aaagaaaata tgcaagaagt gaatatagat
    45001 gtaattacaa taattaataa aagtaatttt tttaaattca ctacacaatc ctccatatag
    45061 ttaagattta cagtataaat tactgcataa acttgtagac atgtaaaaat agattagtat
    45121 ataaaaggat atataaatct acttagaaat ttaaatatcc ttaatatata caatatattt
    45181 aaattataac tcatttatat gtctattaca acaaatttac cctgctaaat acctactcta
    45241 tatcccataa ttttacaagt tgtagtgaag tttaagttct atctcacctc aaatttagca
    45301 gtgtttctat ttacagttct tttatatttc acatctggat aacccaatat aaatgaagtg
    45361 ataacagctt cgttctcgct cattcctaac agttctttta tctttggctc tacatttgat
    45421 gccataacaa aaaatccatt ataacaaatt cctaatcctt gagaataggc cataagttcc
    45481 atattagatg cagccaatcc tccatctaca taagcacttc cacctagaga catatcacct
    45541 acaactacca taagtagtgg ggcatcataa aataaagaat catttccgtt ctctttgtat
    45601 cttttgtaca ttttcttaaa tgtattttta tacatagaac gtacagggtc attgtcatca
    45661 gtatctaatg ccaaattata aagaccttgt attgccagtt ctttcacttc ttttagcttt
    45721 tcttttacta atatgtatct tataggctgt ctattaccac ctgttggtgt gtatctacca
    45781 gcttccacta tgttttttat tttttcttct tctacctcta tatctttata ttgtcttata
    45841 cttcttctaa atttaataaa attcaataac ttttcgggtt ctaattcaaa actttcacga
    45901 ttgtattcta ttacttcatt tttatcaaat ccttgcattt ctattgcatc tgttgggcaa
    45961 attgccatac agtgaccaca gttaatacag agaatatttt taacctttgc tttatcatcc
    46021 ttaactttta ttgcatttac aagacagtct gattcacaca taccacagcc tacacatttt
    46081 tctaaattta tgtctatcat tccaaattat tcctcctttt aaaaaattta ctttagtata
    46141 aaacaaatag ttgtctaagt caatttttat ttttttaaca atatactagc cttaactaca
    46201 ctttaatgga gaaaaatata cataaattgg aacttaaatt agaacttatt caaagtaaaa
    46261 gataaaagaa gtagatgtct aaaagtgaac tttttagtta attagatatt ctttattgta
    46321 tgaaatcaag tggatattta tcatttcaac aataaaaaag gcttacctct attttggata
    46381 tgtccctgtc aagtagacag actaaaaaaa ggctatcttt atgcactgag atagcccctt
    46441 ttcccaacta ctattgttta ttttaatatt atatcaataa atttaatttg ctataacctg
    46501 taagttacat tttaatttga tgtaacttta aattacattt ttaagtagta tctttaaatt
    46561 atattcttaa aatatatcat taaactacat tttaattaat ctaaaaacta tatcttaatt
    46621 aatctaagaa acttacatta taatctttta gtttcccatt atcatataca atctttgcac
    46681 taaagatatt tccagtctta gcccaattta agaacagcct gtcaccttcc cacatattta
    46741 aatcgaatat tttatcatta tcaacccaaa ttaaatttcc ttctttacaa tcatttaaat
    46801 ctttttcaga taattcacct tcaaactcat ctgagaaata caaaaacata taccaatctt
    46861 cttcatcttt aaattttgga aaagttatca accctttata tgacaatgat ttaagagtaa
    46921 gaccagtctc ttcttttact tctctaatta cacattcttc tggagtctct ccttgctcaa
    46981 actttcctcc aactcctaca tacttacctt catgtatatc atctttcttt ttatttctgt
    47041 acagcatcaa agtcttgtca tctttttcta tataacaaat agttgtaagt ttcatgtatc
    47101 tcgccttgct ttctctttta tattctcaac taattatttt cagcactttc aagcacatca
    47161 cttatattct tcattgcatt aagagaaagc tctgcaaatt catcaaatgg aatccctata
    47221 tcttctatac ttctcattgc atctctattt acatttgaag caaatctcgt ttctttcatt
    47281 cttttaataa cagacttagt ctttacactg gtaagttttt tatctggata aactaaagct
    47341 atagcagtta taaacccagt tatagggtct gctgcatata ttgctttttc cattaggcta
    47401 gttcttgtaa ctcccattcc atcatgatga ccaagtattg ctctatacat ttcttcgtca
    47461 ccgaagtttt cttttttaag cagttctact gttgttgtag catgtccttc acatatttct
    47521 cctggctttc ttccaactat atctgaatcc aaatcatgta aaagacctgc cactgcccac
    47581 tcttcttctt tttctggctc tagtcttttg gctaattctc tcataacagc ttcaacagca
    47641 taacaatgtt ttctcattct atcagtttct aaatacttaa ataaaatttc taaagcttct
    47701 tctctcattt tcaccaatcc ccctgttttt tcacatatat gatattttta tatattaata
    47761 cacataaatt caagttttta tatatattca tgtatatttt tcatgttata tctattctac
    47821 acctttatag tagtgtcctg caactgttcc aatttcatct aatttcttat acaagtctgt
    47881 agcctttttt ccaagtttat aattattact caatacctca atgtgagctt caataacctt
    47941 tttaaccatc tcactatctt caactgtaaa atctatccta aatacatcta ttccaatttc
    48001 ctctaactca tatagattgt ccaacataca agtaaccttc gaattgtaaa ttgtacttct
    48061 acagaatata tcttgtgtta tcctataact tgcatccgta aagtctctta aagcataact
    48121 actttcctta catttagcaa ctctcttgtc ctttttacaa tctctaacaa ctactcccat
    48181 aggacagtat tctgtaacca tcataggagc atatccatat attactgctt caatttctac
    48241 atctgtatat tttaaagttt gtttaatttc atttaagttt agttcttgtg acaaacaaac
    48301 tgtacttgct ccctcttctt tgaagtgatt tatactttca ctattgaatg catttaagta
    48361 gcaatctata tttaagtctt ttttcttaaa gtattccata gagccccagt taccaatttg
    48421 aaccttatta attcctttta tattatccaa tatctcaaat tgcttgtact ctttatttct
    48481 aacaatcctt ggtgctgaat agattaattt tttgtcagtg actgctgtat tatagtggtt
    48541 cttattcaaa gtatctattt tacttgtatt tgacaaagta tctatgttat ttgtatttga
    48601 acccattagt tctttaggag tacctaaaga cgaagaagta tctaaatcca tagaaatact
    48661 tgaatctata gaaatatttg aatctataga aatacttgag accatttgta aagcttttga
    48721 taatgtattt atatcttcat aatagattaa atctacatta taatttaata ctgcttctaa
    48781 ttgctccaag ttcttaactt taactctaat cttaggagct gtatcaagtt ttttctgttt
    48841 atttactcta tttggctggt attttaatct attcttctta aattttctat cttttatctt
    48901 tattctttct tcatctaaaa gctcaataca atcccttctc atctgattta acacacttat
    48961 aggcatactt acaccttcat ccaggtcaat ttctatctct tttaacttat atggtgtatt
    49021 tccaagcttt tgaatttgtg tttctatttt ttctttactt aaagccacct ttatagcttt
    49081 ttcaactatt ttgtctcctt ctaccaccga tttatttcca caaaaatcac ttataaaaag
    49141 ctctggcttt ttacctagtt taatactcac ttttgcacgt atattagttt ttatgaactc
    49201 tttatcttgc tgaaatgtag cttgtactct atccattaat tcgctgtcag aagtcttata
    49261 tataagctga tttctctttg cttctccaac aaaatctaat tctatcaaat cccctttatg
    49321 tgctattgta gttatctctc catcctttat tattcttcca atagtacctc cacctaagtt
    49381 aattccatca cctttcttaa gcgtattttc tagcattact ctaagtcttt tgttctttct
    49441 attataatct aaaacttttc caatatatag cccttgatta ttaggtctat tagagttcat
    49501 tatatcctta ccaacttcac ctaatatata tccttttgta aactttctat taaatatggt
    49561 atacaaatta tttattattt cctcagaaat acttattttt ttcttatcta caaattcatt
    49621 aatcgcttct ctatatcctg aaaccacagt agctacatac tctggctttt tcattctacc
    49681 ttcaattttt aaagataata cacctgcttc aattatttta tccaaatctt ctatagtact
    49741 taaatctcta ggactcaaca aataatctcc attacttttt actacttctc ctgtatttat
    49801 atctatcaac tcatactttt gtctacatgg ctgtgcacac ctacctctat ttcccgacct
    49861 attgcctatc atactactca taaggcattg acccgagtaa catacacata aagctccatg
    49921 tacaaaaact tctatatcta cagttgtatt ttcacaaatc cattttatct catcaacatt
    49981 taattctctt gctaaaacaa ctctatcaaa ccctacactc tgaaggtatt ttacatcttc
    50041 taaggaatgt gctaccattt gagtgcttgc atgtagttca aagtcaggta atagtttttt
    50101 tataagtctt gccattccta tatcctgtac tattaaagca tcaatatcta tatcgtataa
    50161 aaattttaca tactctacaa aatcttctat ttcatactgt tttatcaatg tatttacagt
    50221 aacaaagact tgcactcccc ttatatgaga gtatttaaca gcctcaatca gctcatttct
    50281 atcaaagtta tttgcagatg ctctagcact aaagtcttta ccacctaaat acacagcatt
    50341 tgctccgttt tgaactgctg cttttaatga atcaaaagag cctactggtg caagtaattc
    50401 tatttttttc atatttttct cctctattta tacttgttag gttaatataa ttatatctat
    50461 aacttaaata tttgtattaa atttatatat ttcttactat attttaaatt cttcaatcaa
    50521 tttatatatt agttatttat atttggttca tttgagttaa catatacatt atttactata
    50581 tttagtatat ttctattaac ttatatattg attactgtat tttaaattct tcaattaatt
    50641 tatatactgc ttactaaatt taaaacattt taattaattt gtacactgtt tac
//
LOCUS       DAGSRM010000021        49960 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.021, whole genome shotgun sequence.
ACCESSION   DAGSRM010000021 DAGSRM010000000
VERSION     DAGSRM010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 49960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49960
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.021"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            490..1818
                     /locus_tag="MJE33_001321"
     CDS             490..1818
                     /locus_tag="MJE33_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBY9647262.1"
                     /translation="MGSNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGEKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIVIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMVVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPANELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYGIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGVKR"
     gene            2571..3404
                     /locus_tag="MJE33_001322"
     CDS             2571..3404
                     /locus_tag="MJE33_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBY9647263.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFIVVGVRVSSLNMKENEIS
                     RNAKLSSSTITDIVSNTSIEVSKGPSKSSGKIAYITIDDGPSKFTDEMIKTLNKYNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRIPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIVQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
     gene            3497..4429
                     /locus_tag="MJE33_001323"
     CDS             3497..4429
                     /locus_tag="MJE33_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBY9647264.1"
                     /translation="MVGILSLMMVGCNKSLAKVNDVEITKEQYKKTKAVLSATNNYIN
                     GQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVDSKYQELED
                     KMNLNTSYKEKMDKAGVDKDYLKQEISRDLAIDKNKKAFEDRINISDNDMEAYYTSHK
                     KDFNVEEVSASQILISTLDKNKKEVSKDKKEALKKKADNILTKIKNGESFESLAKKYS
                     DDKATGKNGGQLGYFTKDDKNAEFTKEVFKLKKNEVSNVFETSYGYHIVKVTDKRERQ
                     KSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            4481..4963
                     /locus_tag="MJE33_001324"
     CDS             4481..4963
                     /locus_tag="MJE33_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBY9647265.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQEAMY
                     KALYSVENIKEVNYIKTWFYKILVRTSIDFIRKNRKYNNMTDIDLIDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            4975..5844
                     /locus_tag="MJE33_001325"
     CDS             4975..5844
                     /locus_tag="MJE33_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBY9647266.1"
                     /translation="MTSKERLNKLKDEYHKIPIPKKLDTIINHEKIENIYREKNKKVN
                     RLRFKVAIAFACIFTVLVNISPVFADNFSKIPVIGAIVEVITIKNYSLKSENYEAEID
                     IPKIRGLKDKNLEQRLNSSFMEDGKRLYHQFQERMEKIQSSKNKGYKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKVHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKKDSTKSYFIDQKKDLPVEDFKTINKYQDFYFNKNEDLVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            5887..6642
                     /locus_tag="MJE33_001326"
     CDS             5887..6642
                     /locus_tag="MJE33_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBY9647267.1"
                     /translation="MTTYKKLKKSIFKIAIFSICLLVSLVDTNPIFAGSSFNETSNSD
                     TIKVLNTKSINFKQDNYEVNVSIPKIKGLKDKTLENKLNNEFLQDGKKIYSEFQNKMK
                     KEDNKGHRYLNVIHNVKNNSKDFLSIEMTKEEIEASSSITKKHYTVDKNRGILLNLPI
                     FFKDSRYIRAISDNIKEQMRNQIKKDDTKSYFIDQDKDSPVKDFESINKYQDFYFNKN
                     GNLVISFDEYEVAPGYMGAVEFVIPNKVIKRLR"
     gene            6684..7505
                     /locus_tag="MJE33_001327"
     CDS             6684..7505
                     /locus_tag="MJE33_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HBY9647268.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANHER
                     FPESEVVVLDSFENIPDFPTDKNSYIIILTRGHLYDSSALEWALKREAGYIGMIGSRT
                     KIGLTYEKLMKKGFKKEELSKVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            7510..8271
                     /locus_tag="MJE33_001328"
     CDS             7510..8271
                     /locus_tag="MJE33_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBY9647269.1"
                     /translation="MNTGSKDIDTLKVNFRGIEIKAVLVYKNRKNISIKIDPFGKITV
                     MSPPKISKKIIKDIIIEKGDWILKKSEKCKGREEVYKQRMFITGEKFLYLGEEYHLVI
                     KELLRDSVKKSNCRITINEYQIVVQTNDTSTDFIKKSLKSWYKMESERIVLERIDFLK
                     LNCEIMKQLVPASVKVKEQNKRWGSCTAQKNIYINSKISMARLDVIDYVIIHEFSHLV
                     HMNHSKKFYDLVKSIMPDYKDKENWLKINGYKIII"
     misc_binding    8432..8689
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8802..9041
                     /locus_tag="MJE33_001329"
     CDS             8802..9041
                     /locus_tag="MJE33_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBY9647270.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMRH"
     gene            9345..10340
                     /gene="ilvC"
                     /locus_tag="MJE33_001330"
     CDS             9345..10340
                     /gene="ilvC"
                     /locus_tag="MJE33_001330"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HBY9647271.1"
                     /translation="MKMARMYYEKDVDLEVLKNKKVAVLGYGSQGHAHAQNLRDNGVH
                     VVIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLTMMLMPDEKQKKVYEESVKDNLKE
                     GQTLAFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVFTKGSGVPALFAVYQDYT
                     KKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVLCGGISELIKLGYKTLVD
                     AGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEYGDYVSGKRVITDVAKQG
                     MQNVLEDIQNGKFAKAWIKENEEGRENFLKTREEEYNTEIAEVGRNLRSMMSFLK"
     gene            10393..12081
                     /gene="ilvB"
                     /locus_tag="MJE33_001331"
     CDS             10393..12081
                     /gene="ilvB"
                     /locus_tag="MJE33_001331"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HBY9647272.1"
                     /translation="MNGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFKHI
                     RTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMVVISGQVP
                     TSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFRVANSGRKGPVLVDVP
                     KDLFLAEMDFSGEDYDLCQIDDYMDYKSDFDLDDETNIKLLNEAIDIIKESKKPIIYA
                     GGGVKSSDSEEILEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLALS
                     NSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLVGDVKLVLS
                     LLIERVESKNNSNWKEEIKRFRKSEGVQTYEFHPQNILKKINEKYETLKKPTVVVTDV
                     GQHQMWAAKYWNFKGNKSFITSAGLGTMGFGLGAAIGTKVGNVDKNVVLVTGDGSFRM
                     NCNELATVANYNVPMLILLLNNRTLGMVRQWQKLFSNQRYSQTDINENVDYVKLVNAY
                     NIDGYKVSSMEELGKALDMIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
     gene            12292..12969
                     /locus_tag="MJE33_001332"
     CDS             12292..12969
                     /locus_tag="MJE33_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBY9647273.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNAVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNRDFGVTEDSKLYFDLSTPGPNHDTKIDINPPS
                     FEKPLKK"
     gene            13143..15179
                     /locus_tag="MJE33_001333"
     CDS             13143..15179
                     /locus_tag="MJE33_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HBY9647274.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNDEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLENVSEKWNTSRDILIKS
                     GDEIIEALKDDFGVKNTEGDLSKEMLSSSVRVFKAIYDEKYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITKKELYKEIAIKTIDYVVREMKDKEGGFYSAQDADSEGEEGKFYT
                     FNPLEIIEVLGEEDGTFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIADLSKKV
                     FEYRKERTSLHKDDKILTSWNALMIVALTKAYSTLKNDIYLEYSNKCLNFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNESCINLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDNRLEEMSYKQ
                     LKLYVDNVKSSPTGYSFYMLSLMFELYSTKEIICIFKEDSDLIAFKELISENFIPNAT
                     FLAKKYNEENTIIGFLNNYRLKDDKISYYVCQSNSCSQPINDLQKLKDMILGVE"
     gene            complement(15370..15936)
                     /locus_tag="MJE33_001334"
     CDS             complement(15370..15936)
                     /locus_tag="MJE33_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBY9647275.1"
                     /translation="MDYSNELKELFLMNQTYATLFTLTNKIQIEGDKYFGILTSRQYM
                     TILSILHLPEEETTLNNIARKMGTSKQNINRLVANLEKNGYVDVIPSPHDKRAINVKV
                     TDLGKKVMVTCSRTGINFMADVFHEFTKDELETLWSLLKKMYRFNGEEQDGFEEDANF
                     MEYEEIDKIKSEALEEFAKRRNRVNKKD"
     gene            16224..17159
                     /locus_tag="MJE33_001335"
     CDS             16224..17159
                     /locus_tag="MJE33_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBY9647276.1"
                     /translation="MKCTNFTFKGEEGLDIYTYKWEDENIKKPKAVIQIAHGMAETAQ
                     RYETFAKVLTKNGYIVYINDHRGHGKTAKIIENVGHLAEKEGFRCLVEDMYTLTNIIK
                     KENEDLPIYLFGHSMGSFASQRYIMDYSNNLSGLILCGSNGKQGIILNLAHLIINREI
                     KKYGRRSKSNKINNLIFGGEIIRRNEKTKFDWLSRDKEQVEKYINDPFCGVVCSCGFF
                     YDLVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGKGVLRLRDRYIKLGVKDVT
                     CKLYKDGRHELLNEINREEVFEDIICWLNNKIEIL"
     gene            17630..18844
                     /locus_tag="MJE33_001336"
     CDS             17630..18844
                     /locus_tag="MJE33_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY9647277.1"
                     /translation="MQIKIGKVIQCLRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEEVMKIYKELESGFARIEIDLSTEEPKEEF
                     RQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELNDRVKETTNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTSVNLAMIYLKQNRL
                     EEAEILLQNKLFSNVFNISMDCKGIINVYKNQYKELKKKLENRNFNKNAIEREMEYIK
                     NKLLGYANLSLEIKKMLSEDKGAFFSMYMDYMELSLIFLFFNMKEEAKKALYSLKEIL
                     EKYPIHESLDVSQMRFFDKVESKNLYTFNIYTNLLIVLNDDSYNELREEPIFKDVIEK
                     ILDMEKMLKNKE"
     gene            complement(19264..19785)
                     /locus_tag="MJE33_001337"
     CDS             complement(19264..19785)
                     /locus_tag="MJE33_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY9647278.1"
                     /translation="MKFEWLRTSENKLYYKAMELYKISFPFHEQRKSSLQAEILKNKE
                     YQFNLIYDKNQFVGIILCWETDDFIYVEHFCIFPEMRNNRYGQRALELLNKKGKTIIL
                     EIDLPIDEISIYRKAFYERVKYKTNNYEHIHPPYHKKFNGHNLIVMSYPEKLSKMEYD
                     KFNHYLRNNVMSF"
     gene            20141..20938
                     /locus_tag="MJE33_001338"
     CDS             20141..20938
                     /locus_tag="MJE33_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBY9647279.1"
                     /translation="MEKREKRIIISSLLSVSILMGLVSIYSILNKEDIILTVKGQEQK
                     VSSFKKTVEELLDEQGVKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFD
                     TKHVNDSNLLKGKSKVYQEGQEGEKKLVYNLTYHDGKLVKKVLSKEVISKEPTTKIIK
                     YGTKEKVLIASRGANIRGGKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            21048..21638
                     /locus_tag="MJE33_001339"
     CDS             21048..21638
                     /locus_tag="MJE33_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBY9647280.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNKETRLEIVNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGAGNEIINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNVREDWSCFTDFNIVSQNLESYSYWIKKFCMTFNTNFVDFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            21704..23401
                     /locus_tag="MJE33_001340"
     CDS             21704..23401
                     /locus_tag="MJE33_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBY9647281.1"
                     /translation="MDYTKTYEEWIKGSYFDEDTKLELENIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANSITEKVNSIHDYSTIPTLTEANKNLVVLLDESQDTKFIEAVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNVPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKANNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGMSLYDGLIELYKK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSIKVVDKKDYKNGIDNLPKS
                     DVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDDMVEKLK"
     gene            23615..24454
                     /locus_tag="MJE33_001341"
     CDS             23615..24454
                     /locus_tag="MJE33_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBY9647282.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFFKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLVGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIRHE
                     MLK"
     gene            24971..26827
                     /locus_tag="MJE33_001342"
     CDS             24971..26827
                     /locus_tag="MJE33_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBY9647283.1"
                     /translation="MDTHNKYVNFIKNIPVPFLYCRIVKRQEDIEYRVEYISKGMGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIIGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGIYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGENTGKLFRDDYREGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIVDNIIYSGYSNRNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEALIDKIFESDKGVVENKKVVTFELNISMNDENKCIEITKRPFFD
                     SYGSVIGIIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKKDGKINIFL
                     YVKDNVFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGIGSEFIVSLPVRSKNNIEKYNHKREISNELSKKLEIEFSDL"
     misc_binding    26910..27150
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    27216..27451
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            27536..28753
                     /locus_tag="MJE33_001343"
     CDS             27536..28753
                     /locus_tag="MJE33_001343"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBY9647284.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRNIEIVGILVNNLEK
                     HKDKKYSNIITNNIDDIFNKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     gene            28870..29352
                     /gene="cdeM"
                     /locus_tag="MJE33_001344"
     CDS             28870..29352
                     /gene="cdeM"
                     /locus_tag="MJE33_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HBY9647285.1"
                     /translation="MENKKCYSEDWYERGESTAKWFQNDREEYEREAYDEDRERRGSN
                     CGCSDSGENRPRNCERFRREAEIREREAREAFCESSEKKKEALAYECEARKLWEEAEK
                     YWDEYSKYNYKGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCNCCRK
                     "
     gene            29617..30882
                     /gene="hisD"
                     /locus_tag="MJE33_001345"
     CDS             29617..30882
                     /gene="hisD"
                     /locus_tag="MJE33_001345"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HBY9647286.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEVAQKVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(31085..31837)
                     /locus_tag="MJE33_001346"
     CDS             complement(31085..31837)
                     /locus_tag="MJE33_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBY9647287.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSNMFKALVPLYAE
                     NFRCILIDFLGNGQSDRVSQFSPDIWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCHGKDWEN
                     VVNLDTKALLQCANEKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMATFIS
                     KASIYIFSQGGHPAILTNADEFAQLAKVFFKY"
     gene            complement(31955..32224)
                     /locus_tag="MJE33_001347"
     CDS             complement(31955..32224)
                     /locus_tag="MJE33_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647288.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKKHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
     gene            32699..33793
                     /locus_tag="MJE33_001348"
     CDS             32699..33793
                     /locus_tag="MJE33_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBY9647289.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISSITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKKEKGNNIRAIGIGVPGVVKKGVVKNI
                     PTIKSWEGINLENLIKSKYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKRYLESKDNKFKK
                     EILSIISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNNVGEENFPKLIKVDS
                     LREGSINGVISMCLKETDYQYSLSNKKGGY"
     gene            33795..34745
                     /locus_tag="MJE33_001349"
     CDS             33795..34745
                     /locus_tag="MJE33_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HBY9647290.1"
                     /translation="MKYLVSIDGGGTKTKFCVSDLDGNILKEHTTGSTNYKSVGIKKT
                     YENINNGFKKILKDLYIDYDDIEYTVFGISGCDSPNDYKIIMDEILKIGINKEKIYLA
                     NDAVLAFYAQADSPGLVIVAGTGSIILGIKEDGEIYRVGGWGYNFSDLGSGYDIGRKL
                     LKKVLLYCDECHEYSDLFSCVLDFFGANSFEQLAYMITDINDNVEIANLASLVIDCAE
                     QGDKLAIEILRESSTELSKLAQVILRKISNPMKMDKSEINIVLSGGTLNKTVYAQMLM
                     NNLKTENTDKNLKFISQENQPVYGGIRLAMALANRRVKHG"
     gene            34738..35922
                     /locus_tag="MJE33_001350"
     CDS             34738..35922
                     /locus_tag="MJE33_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647291.1"
                     /translation="MDRKKVIVQIFTGGFKAKEIKFNDIKCKLLELLDCIDIEKVIMG
                     WSVDEELYVETKKLLNKYNVELYLWLPCFSEIGLLEKSNLLIDYMGKEVKGYALQEDE
                     NFEFYCPNNKENIKNIKKIYSTYFSDIKFDGIFLDKIRYGSFSNKLNGVFNCFCKDCL
                     NIYKKNNIDKESLMREMNNVSKGVIDNEDTPFGITEYINGKYKFKNKIWEDFFETKNQ
                     NIYKALLEISSYFKSKGLKIGIDAFSPFISYFTGQDLEKLQEVADFIKPMMYRTTKAP
                     AGLPFEFERFIDESIHKNKEIGKRNFLDILGIKEYEGDVYPIEFVLEEIDFMTRNLNK
                     DIYVGVEINKKEIISPVTPKYISENISNINKTTVKGYVLSWDLLSAPKENIDEIIKWF
                     RN"
     gene            35935..37431
                     /locus_tag="MJE33_001351"
     CDS             35935..37431
                     /locus_tag="MJE33_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBY9647292.1"
                     /translation="MSKILEIQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILTGNEIMFKNPHHSKENGISIIYQELSLIPTLTVLENIFL
                     GREILKGSVFLNKKDMLEEYMKICEDFDFNIDPHIVVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKDGLFKIISRLKSKGKTIVYISHILEEVFKVCDSASIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLIEKKDYTFAKYEEPPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEIEFSKKGVKELGIKVSD
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSKGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            37454..38404
                     /locus_tag="MJE33_001352"
     CDS             37454..38404
                     /locus_tag="MJE33_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9647293.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVAALAGAITMKFATGGTNLFASMFLGVAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFN
                     IPIPVVIFFIVVLASFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            38425..39387
                     /locus_tag="MJE33_001353"
     CDS             38425..39387
                     /locus_tag="MJE33_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBY9647294.1"
                     /translation="MKKLMKHLALLLSLVMIFGLVGCSNGGDKEKNGEKKIAVLLPGS
                     TGYFVATKQGIDAKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIVKAANDSDVPIVAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     TGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGLKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVEEGGLVIEKASKFLKGEKIEPVTQIKQVEVNKDNISEFKGEW"
     gene            39759..40391
                     /locus_tag="MJE33_001354"
     CDS             39759..40391
                     /locus_tag="MJE33_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBY9647295.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDSLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            40802..40894
                     /gene="kdpF"
                     /locus_tag="MJE33_001355"
     CDS             40802..40894
                     /gene="kdpF"
                     /locus_tag="MJE33_001355"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HBY9647296.1"
                     /translation="MEGLPMWFLAVVIIMLIIYLVYALINPEKF"
     gene            40935..42617
                     /gene="kdpA"
                     /locus_tag="MJE33_001356"
     CDS             40935..42617
                     /gene="kdpA"
                     /locus_tag="MJE33_001356"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HBY9647297.1"
                     /translation="MFMVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDN
                     FIYKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGL
                     SFNTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGLVGKK
                     KTLGNFYVDLIRITTRVLLPGALIIGLILVTQGVPQTFAGTETVTTIEGKLQDIARGP
                     VAALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGHMIKN
                     KKQGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSS
                     LFTTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVF
                     LCGLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGISNPGF
                     HGLSQVLYEFASSAANNGSGFEGLGDNTMFWNITTGIVMFFGRYVSIIVLLAISSLLA
                     SKKAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            42642..44699
                     /gene="kdpB"
                     /locus_tag="MJE33_001357"
     CDS             42642..44699
                     /gene="kdpB"
                     /locus_tag="MJE33_001357"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HBY9647298.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNAGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMVL
                     NLG"
     gene            44718..45344
                     /locus_tag="MJE33_001358"
     CDS             44718..45344
                     /locus_tag="MJE33_001358"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HBY9647299.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTGVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDVRFFKGRVSSVNYNTYTKEDLVPDKDGNTSYGGVSSGSFNY
                     GATNPELHDRVQKDIEQFLKDNPTVKREDIPTDLLTASGSGLDPNISPASAKIQIPAI
                     AKASGISESKLQKIVDDNTSKKLFGVLGEDRVNVLKVNVEVAKILGLI"
     gene            45581..46489
                     /gene="cysK"
                     /locus_tag="MJE33_001359"
     CDS             45581..46489
                     /gene="cysK"
                     /locus_tag="MJE33_001359"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBY9647300.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEQRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            46516..47106
                     /gene="cysE"
                     /locus_tag="MJE33_001360"
     CDS             46516..47106
                     /gene="cysE"
                     /locus_tag="MJE33_001360"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBY9647301.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVSYEYDELDNVYYI"
     gene            47266..47454
                     /locus_tag="MJE33_001361"
     CDS             47266..47454
                     /locus_tag="MJE33_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBY9647302.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(47508..48377)
                     /locus_tag="MJE33_001362"
     CDS             complement(47508..48377)
                     /locus_tag="MJE33_001362"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HBY9647303.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDINKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDAINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTKMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(48574..49818)
                     /locus_tag="MJE33_001363"
     CDS             complement(48574..49818)
                     /locus_tag="MJE33_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647304.1"
                     /translation="MENNLNTAKRNNTELLQNDSLKVNLQIGVDFTLLSKSLKAFYKK
                     EKDFYCIALAPCNVSSEKANRKISIYDMIKEINILIESITDKNELLSEELFTNNLSEF
                     YDESLDEIYIDLKQAYLYIKKNTDSLNIEDSIEYVFDVDISNDIKAKENPLINFNSLS
                     LVVWSGETKHILQQMNILDLEDIHSYSIENNTNITIYENNTLQYKFNEESENIKDSSI
                     DIIEQMLILPDDLNYDKDEVENMKNRLAKINIKYLQTLKEKDIKIKLINSNLTDEPEF
                     SDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYANVHSSKNLELHETAHA
                     IDKNVLNKKSNSEEFMEVFAQERYKLYDPKQVAHAYISKFIEEFFAESFVHYYLDEDS
                     KNTLKENCPLTYDFLEKLELNY"
BASE COUNT        19723 a         5129 c         8767 g        16341 t
ORIGIN      
        1 tttttaaata aaaaaataaa atattagttt tgatttctgg tttaaattgg gttataataa
       61 attgtcaaat tatttattaa tataaaatag actataaaac aagcaaaaat catactatta
      121 tataaaagac cttaaagata gaaaactaca gaaagaagcc aaaatgtttt tgaaaaaaat
      181 gaaattttat tgcaaaaaaa tcaaaatgaa tttaacttaa tagttataat ctatttttag
      241 tgttataaat atacaatgta tattatacaa tataaaaaat acatttactt agaagtatta
      301 ttatatttgg atttttggtt tttataattt ttttatattt aaacataact aaaaataaaa
      361 gattactaaa gctaagtaga gtttatttta ttttaataaa taataaagct atttttctat
      421 atattggcat aatatatgct tatttaacta tgaaattaaa attaaatagg attaatgagg
      481 aggaaaaaaa tgggaagtaa tgaactaaaa aagactcttg gcgtatcagc agcattatca
      541 actgttgttg gttccgttat aggagcgggc gtatttttta aaccacaagc agtctataca
      601 ttaactggag gagctccagg tcttggtata ttggcatggt taatagctgg aatcataact
      661 ataacagctg gtcttacagc tgcagaggta tcagttgcga taccaaaaac tggtggaatg
      721 atggtatata taaaagaaat ttatggagaa aaattaggtt ttttaactgg atggatgcaa
      781 attgtattat tttatccagg gatgatggca gcattaggtg ttatatttgg agaacaagca
      841 tcagcactaa ttggtagtcc atcactttta cttccaatag caattggaat tatagtaata
      901 gttgcagggc ttaatatgtt aggttcaaaa actggaggag tcatacaaac tgtttctaca
      961 atttgtaagt taataccact tattttaata atggttgttg gatttattaa aggtggtgga
     1021 aataatccaa tacttacacc tatggttggt gaaggattaa gcctgggtag tgtactaggt
     1081 caagttttaa ttgctatatt atttgctttt gatggatgga tgaatgttgg tactttagct
     1141 ggagaaatga agaatcctgg aaaagacctt ccaaaagcta taataggtgg gttgtcagtt
     1201 gttatggctg tttattttat aataaattta gcatatcttt gggtattacc agctaatgaa
     1261 ttagcaaatt atgcttctcc tgcatcagca gttgctgaag ttatatttgg ttcaatgggt
     1321 ggaaaaataa tatctgtagg tatattaatt tcagtgtttg gagcattaaa tggattttta
     1381 ctaactggtt caagggtggc atatacttta gctacagata agactttacc aaagtatggt
     1441 atattttcta aattaaattc tgctcaagtt ccagcaaatg caatagcatt agtatctgtt
     1501 attgcatcta tatatgcttt gtcaggtcaa tttaatttat taactgactt agctgtattt
     1561 gctacttgga tattctatgt attaactttt ataggggtta tgaaacttag aaagactcat
     1621 ccaaatatac caagagagta taaagttcca ttatatccta ttgttcctat aatagctata
     1681 gcaagtggta tatttgttgt agttaatcaa ttgtgttttg caggtatgaa aactactatg
     1741 atatctatag gaggtcttgt aataacagct ataggcttac cagtgtatgc ttatatgact
     1801 agaggagtaa aaagataaac caagtagttt acaaatgctt cataattaaa ataaaagaga
     1861 ctgtctcaaa tagagataag tctctttttt attgttaaaa tggtaaagat atgtttgatt
     1921 ttacaaaaaa agagcgttcc atgaactaaa aagtttattt taaggtattc attttatttt
     1981 tagttttttg attataaaat taaacatcta aaaaattatt tattttaaaa tactttattt
     2041 taaagtgttt tttatcaaac taagtggtat ctgtacaaca aatattatac atagctttta
     2101 tgaaaattaa atctttctgt tatatataat agaatattgt attatataga aaatattacc
     2161 tgattaatat agtgatttat aaatgagatt agaaaatttt atttgataac aaaaattaga
     2221 tagtatgaat atataatgta tgtattaaaa atgtggcaaa tagttgtttt gctatttatt
     2281 atttgtttaa tatttctaat ttataaaaaa aaaattataa attttaaaaa ttataaatga
     2341 cattttaact agaattaatt gtaaaaaaca ctatataata ctcaattttc aacattctat
     2401 acaattatac agaaattata cagcataata atcaccgaaa taacaaattt taaaaattaa
     2461 gcaaaacttt ttgtcgaagt tcaacgtcta tatatatgta gtgtaaattt ttgggataaa
     2521 atactacaaa aatataaatg taaaaatata gtaatattgg aggtcgaacg atgagaaata
     2581 aaaagaaaaa aataaataga aagaagttgt atttgctttt aagtgctatt gtagtgtgtc
     2641 tggcttttat agtagttggt gttcgcgtca gctctttaaa tatgaaggaa aatgaaatta
     2701 gtagaaatgc aaaactgtca agttctacta ttacagatat agtatctaat acatcaatag
     2761 aagttagtaa agggccaagt aaaagttctg gaaaaatagc atacataact atagatgatg
     2821 gtccatctaa atttactgac gagatgataa aaactttaaa taaatacaat gttaaagcaa
     2881 ctttttttat gatagatggg aatatgaagg aatatccaca gcaagttaaa aatataatta
     2941 aaaatgggaa cacagcaggt tttcatagtg tatcacatga tattcacaag ctttatgtta
     3001 ctagtacatc agcaaaagag gaatttgata caaatgatca aactttttat aagataactg
     3061 gtaaacattc aaaagtaata agaatacctt atggaagcaa accatataca ccacaagcct
     3121 cttatcaggc tttagttgat gctggatata aaatttggga ctgggactta gatacagaag
     3181 actggagatc taattcttct caaattgtac aaaatgtaaa aaatcatatt aagaatagaa
     3241 aaggtgaaga taaagaccaa cttgtagttt taatgcatga gaaaaaacag agtgcagaag
     3301 ctttagattc agttttaaaa ttcttatcag atgaaggtta tgaatttgcg ccagtagatc
     3361 aaaaccaaat accaaaaaat tattggttac gtaatttaga gtagttaaaa tataagaata
     3421 ttgattgata tttttagaaa ttgagaataa aaatgagtga ggtgtaggta tattgaataa
     3481 aaaactttat ataggaatgg tcggaatatt gtctttgatg atggttggtt gtaataaaag
     3541 tttagcaaaa gtaaatgatg tggaaatcac aaaagaacag tacaaaaaaa caaaagcagt
     3601 tttatctgct actaataatt atataaatgg gcagtcttta gatgaattag aaaaaacttt
     3661 ggataaaaaa ggtagaaata aattagaaaa tgttataata tcgtttatgg tggataatga
     3721 acttttatat caagaggcta aggataaagg actaactcca agtaaaagtg aagtagactc
     3781 aaaatatcaa gaactagaag ataagatgaa tttgaataca agctataagg aaaaaatgga
     3841 caaagcaggt gttgataaag attatttaaa acaagaaata tctagagatt tagcaataga
     3901 taaaaataaa aaggcctttg aagatagaat aaacataagt gataatgata tggaagccta
     3961 ctatacaagt cataaaaaag attttaatgt agaagaagta agtgcttctc agatacttat
     4021 atcgacttta gataaaaata aaaaagaagt aagcaaagat aaaaaagaag ctttgaagaa
     4081 gaaggcagat aatatattga ctaaaatcaa aaatggagaa agttttgaaa gtttggctaa
     4141 aaaatattcg gatgataaag caactggaaa aaatggagga cagctaggat attttacaaa
     4201 ggatgataaa aatgctgagt ttacaaagga agtgttcaaa ttaaagaaaa atgaagtttc
     4261 aaatgtgttt gaaactagtt atggatatca tatagtcaaa gttactgata aaagagaaag
     4321 acaaaaaagt tttaatgagt gtcagagttt aataagagaa tctattttaa atgaaaagta
     4381 tattgagcat ataaaaaaac tgaatgaaga tgctaaaata gacagataga atttctaaca
     4441 gctcaatcct agcctgtaat cggagggaga ttttacagga atgtttaaga gtttaaagag
     4501 aacgaaagtt gagaaataca ttatcgataa taaagactct ttttatagaa tagcatatag
     4561 ttatacgaag aatgaagaag atgccttaga tgtggttcaa gaagccatgt ataaggcact
     4621 atactcagtg gaaaatatta aagaagtaaa ttatataaag acatggtttt ataaaatttt
     4681 agttagaacg agtatagatt ttattagaaa aaaccgtaaa tataataata tgacagatat
     4741 tgaccttata gatgagactg gagagtatga taaatatact gatttggatt taagaagagc
     4801 tttggaagaa ttacctattg aatacaaatc tattataata ttaagatttt ttgaagattt
     4861 aaagattgaa gaagtagcaa taatattgga tgaaaatgtg aatactgtta aaactaggtt
     4921 atatactgct ttaaaaaaac taaaattaaa aattgaagag tagggataaa aagaatgaca
     4981 agcaaagaaa ggttaaataa gttaaaagat gagtatcata aaattccaat acccaagaaa
     5041 ttagatacta taataaatca tgaaaaaata gaaaacatat atcgagagaa aaataaaaaa
     5101 gtaaatagat taagatttaa agttgcaata gcctttgcat gtatatttac agtattagta
     5161 aatataagtc ctgtatttgc agacaatttt tctaagatac ctgtaattgg agcaatagta
     5221 gaagtaataa ctataaaaaa ttatagttta aaaagtgaaa attatgaagc tgagattgat
     5281 attcctaaaa tacgaggttt aaaagataaa aacttagaac aaagattaaa cagtagtttt
     5341 atggaagatg gaaaaaggct ttatcatcaa ttccaagaga gaatggagaa gatacaatca
     5401 tcaaagaata aaggatataa atctttaagt ttgagttata gtgtaaaaaa taattctaaa
     5461 aaatttctat caatagagat gacaaaaaat gagatagagg cttcatcata tgtgagtaag
     5521 gtacattata ctattgataa aaaaagacaa atagttttga ctttgccaat gctttttaaa
     5581 gatgataagt atataaaagt tataagtgat aatataaaag aacaaatgag agaacaaatg
     5641 aaaaaggata gtacaaaatc ctattttata gatcaaaaaa aagatttgcc tgttgaagat
     5701 tttaaaacaa ttaataagta tcaggatttt tattttaata aaaatgaaga ccttgtaatt
     5761 tgttttgatg aatatgaagt agcacctggt tatatgggtg cagttgagtt tgtaatacca
     5821 tataaagtaa taaaagattt gtaaattgaa tagtagttta tatataaata ttttaagaag
     5881 gacaatatga ctacatataa aaagcttaaa aaatctatat ttaaaattgc tatattttca
     5941 atatgcttat tagtttcatt agtagataca aaccctatat ttgcaggtag ttcctttaat
     6001 gaaacttcaa atagtgatac gataaaggtg ttaaatacta agagtatcaa ctttaaacaa
     6061 gacaattatg aagtgaatgt gagtattcct aaaataaaag gtttaaaaga taaaacttta
     6121 gagaataaat taaacaatga gtttttgcaa gatgggaaaa aaatttacag tgaatttcaa
     6181 aataaaatga aaaaggaaga taataagggt catagatacc taaatgtaat ccacaatgtc
     6241 aaaaataatt ctaaggactt tttatcaata gaaatgacaa aagaagagat agaagcttcc
     6301 tcatctataa ctaagaagca ttacactgtt gataaaaata gaggaatact attaaattta
     6361 ccaatatttt ttaaagatag tagatatatt agagctataa gtgataatat aaaagagcag
     6421 atgagaaatc aaattaagaa agatgataca aaatcttatt ttatagacca agataaagac
     6481 tcgccagtga aagattttga atctataaat aagtatcaag atttttattt taataaaaat
     6541 ggaaatcttg tgattagttt tgatgaatat gaagtagcac ctggttatat gggagcagta
     6601 gaatttgtga taccaaataa agtaataaaa agactaagat agatatatag aagattttta
     6661 gatagactgg taaggagaat tatatgaata tatatgaaca agcaataaga ttaatagaaa
     6721 ataatgagga tttcgcattt gctacaataa cgtctcattc aggttcaaca ccacgtgaaa
     6781 ctggagcaat gatgattgtg aaaaatgatt ctactatatt tggctcagta ggaggaggta
     6841 gtgtagaagc agaatgtata aaacatgcaa ttaatgttat aaaaaacaga gaatcaatgt
     6901 tgtataaatt tactctaaac aaatctgatg tggcaaaact aggaatgata tgtggaggta
     6961 ctggagaaat acaaatagat tttatagata gtaaattaaa aagtaatata gaaaaattta
     7021 ataagagact aaaggaaaat acaagtaaag catatatatt tggtgcaggt catatttcaa
     7081 gagatgttgc tgtgatacta tctcttttag aatttagaac agtagtaata gatgatagag
     7141 aagaatttgc taatcatgaa aggtttccag aatcagaagt agtagtttta gattcttttg
     7201 aaaacatacc agattttcca actgacaaaa atagttatat aattatttta acaagaggtc
     7261 atctatatga ttcaagtgct ttagaatggg ctcttaaaag agaggctggt tatataggta
     7321 tgattggaag tagaacaaaa attggattga cttacgaaaa actcatgaaa aaaggtttta
     7381 agaaggaaga attatcaaaa gtacatgcac caataggcat aaaattgaat gctcaaacac
     7441 cagctgaaat agctgtatgt atagctgctg aacttataaa ttgtagagct aataaggaaa
     7501 aataaactaa tgaacacagg gagtaaagat attgatactt taaaggttaa ttttagaggg
     7561 attgaaataa aagcagtttt agtatataaa aatagaaaaa atatttctat aaaaatagat
     7621 ccatttggca aaataactgt aatgagtcct cctaaaatat ccaaaaaaat aataaaagat
     7681 ataattattg aaaaaggaga ctggatactt aaaaagtcag aaaaatgtaa aggaagagag
     7741 gaagtatata aacaaagaat gtttataact ggcgaaaaat ttctttattt aggtgaagag
     7801 taccatttag ttataaaaga actgttgaga gattctgtca aaaaaagtaa ctgtagaata
     7861 actattaacg aatatcaaat agttgttcag acaaatgata caagtacaga ttttattaaa
     7921 aaatctttaa aatcttggta taagatggaa agtgaaagaa tagtattaga gagaatagat
     7981 tttcttaaat taaattgtga aataatgaag caattagttc cagcgagtgt taaggtaaaa
     8041 gaacaaaata agagatgggg aagttgtaca gctcaaaaaa atatttatat aaactcaaaa
     8101 atatctatgg caagattaga tgtaattgat tatgtaataa tacatgagtt tagccattta
     8161 gtacatatga atcactctaa aaaattctat gatttagtaa aatccattat gcccgattat
     8221 aaagataaag aaaattggct aaaaataaat ggatataaaa taataatctg aaaacgatta
     8281 ttccaaaata taggcgtaaa gtataaaaga agtattattg tgaaaaaaat aaataatctg
     8341 aaaattttaa aaaaagtcat tgacattgta aatgataggg aatataatct atattaacaa
     8401 caaaacagaa ttaaaatata aaattaaaaa attaaaagca atgacagaga caacaatact
     8461 aatagtgtta tccacagaga accaatgttg ctgagaattg gtgttaacag tagtataatg
     8521 atcactctgg agcttttgta ctgaatattt ttagtaagta caaacgtatg tcagcgttac
     8581 atgactaagg ttaaggtaat taattacccg acctgaaaaa agaggggtag atgtattttt
     8641 tatatctaca aataaggtgg taccgcggaa tataactttc gtccttataa gtaatattta
     8701 tattgcttat aaagatgaaa gtttttttat tttaagcgat ataaataaat ttaatccata
     8761 taaaattaat aaaaaaataa taaaaataag gagggatttt tatggaaaat actgtatatg
     8821 ctaaattaaa ccaaggaata gattcttttt taagagtggc tatgactcta agaagaagag
     8881 aggttgatat acaatcaata agtatgactg tagataatat gaataattca ggtataaaat
     8941 taatagtaaa tgaagaaaag acatccttag attctgtctt aaaccatatg aaaaaactac
     9001 atgatataag agagataact gttgaaagaa tgagacatta attaaatata ttttataaag
     9061 ctaaagataa atatgcttat tttaaaataa aaaactaaag ataaatatta aataagaaaa
     9121 atcttagatt aaaatataag tgtcattgaa tttattagtt tatcacagtt aataaattta
     9181 ttagtttgtt gtagctaata aaaactgaaa attctaaaat tataattgta agattatata
     9241 aatttagaat ttaaatctgt tgaataatat taaaacaatg ataatgagta tgaatttcta
     9301 ataaaaataa atatcataaa aataagaaaa agtattggga ggaaatgaaa atggcaagaa
     9361 tgtattatga aaaagatgtg gatttagaag ttttaaaaaa taaaaaggtt gcagttttag
     9421 gttatggaag tcaaggacat gcacatgcac aaaatcttag agataacggt gtacatgtag
     9481 tgataggtct ttatgacgga agtaaatcag cacaaaaagc aaaagaagat ggatttgaag
     9541 ttaagagtgt agcagaagct acaaaagaaa gtgatttgac aatgatgtta atgcctgatg
     9601 aaaaacaaaa gaaagtttat gaagagagtg taaaggataa cttaaaagaa ggccagacat
     9661 tagcttttgc tcatggattc aatatacatt ataatcaagt acaaccacca gaatttgttg
     9721 atgttgtaat ggtagctcct aaaggacctg gtcacttagt tagaaatgta tttacaaagg
     9781 gaagtggagt tcctgcacta tttgcagtat atcaagacta cactaaaaaa gctacagaga
     9841 cagttttagc atatgcaaaa gggataggag caacaagagc aggtgtttta gaaactactt
     9901 ttaaagaaga aacagaaaca gatttatttg gagaacaatc agtactttgt ggtggaataa
     9961 gtgaacttat aaaattggga tataaaacgc ttgtagatgc aggttatcaa aaagaagtag
    10021 catattttga atgtttacat gaaatgaaac taatagtcga ccttatatat gaaggtggat
    10081 ttgaaagaat gagatatagt ataagtgaca cagcagaata tggagattat gtatctggta
    10141 aaagggtaat aactgatgtt gcaaaacaag gtatgcaaaa tgttttagag gatatacaaa
    10201 atggaaaatt tgcaaaagcg tggattaaag aaaatgaaga aggacgtgaa aacttcctaa
    10261 aaactagaga agaagaatat aatacagaaa ttgccgaagt tggtagaaat ctaagaagta
    10321 tgatgtcatt cttaaaataa atatatatta aatggattag tttgagggaa agagtggtgt
    10381 atagtagtgc gtatgaatgg agcaaaggta attttagaat gtttaaagaa agagggaatt
    10441 gacacaatat ttggatatcc tggtggagcc gtgatacctc tttatgatgc tctatacgat
    10501 tattcagatg attttaaaca tataaggaca tcccatgaac aaggtttagt tcatgctgca
    10561 gatgggtacg caagaagtac taatacagta ggagtttgtt ttacaacatc tggtccagga
    10621 gcaacaaatg ccataactgg aatagcaaca gcttttatgg attcttcacc tatggttgta
    10681 atttcagggc aggtgccaac tagtttatta ggaaaagatt cttttcaaga aattgacata
    10741 actggagcaa cattatctat gacaaaacat aattatttag ttagaaacac taaagaatta
    10801 gttcctacaa taaaagaagc ttttagagtt gcaaattcag gtagaaaagg acctgtactt
    10861 gtagatgttc caaaagattt atttttagca gaaatggatt ttagtggtga ggactatgat
    10921 ttatgtcaaa tagatgatta tatggattat aagagtgatt ttgatctaga tgatgaaact
    10981 aatattaagc ttttaaatga agcaatagat atcataaaag aatctaaaaa gcctataata
    11041 tatgcaggtg gaggtgttaa atcttcagac agtgaggaaa ttcttgaaaa atttgcaact
    11101 aagatagata cacccgtact aaatacactt atggggcttg gaaatataga tagaaagaat
    11161 gaattatctc ttggaatggt tggaatgcat ggaagtagag aaagtaattt agcactttct
    11221 aattcagatt tagtaatagc gataggtgcg agatttagtg atagagtgat aagtaaaagt
    11281 tctgaatttg ctaaaaatgc aaaaataata cacatagaca tagacccatc tgaaataagt
    11341 aaaaatatag aatctaatgt atctttggtt ggagatgtaa agctagtcct tagtttatta
    11401 attgaaaggg ttgaaagtaa gaataatagc aattggaaag aagagataaa gagatttaga
    11461 aagagtgagg gtgtacaaac atatgaattt catccacaaa atatacttaa aaaaataaat
    11521 gaaaaatatg aaacattaaa gaaacccact gtggtagtaa ctgatgtcgg tcaacatcaa
    11581 atgtgggctg ctaagtactg gaattttaaa ggtaataaaa gttttataac atcagcaggg
    11641 ttaggaacta tgggatttgg tttaggtgca gctattggaa ctaaagttgg taatgtagat
    11701 aaaaatgtgg ttttagtcac tggtgatgga agttttagaa tgaactgtaa tgaactagct
    11761 acagttgcaa attataatgt acctatgctt attttactac tcaataatag aacattagga
    11821 atggtaaggc aatggcaaaa gttattttcc aatcaaagat actctcaaac tgacattaat
    11881 gaaaatgtgg attatgttaa actcgtaaat gcatataaca ttgatggata taaggtatcc
    11941 agtatggaag aattaggaaa ggcgctagat atgatagatt ttaataaacc agtatttttg
    12001 caatgtgata tagataagga ttatgatgtt tatcctattg tagcacctaa tgatgcattg
    12061 gaaaatttga tatgtaacta aaacttatac tgaatattga taaaattttt tattaaattt
    12121 ttattagtat tcagtttttg ttgtttagag atagattggc attttttatt tttgcaaata
    12181 aatcaatgac tatataatta agaacttatt aaattttaat tatacaattt agaatagtat
    12241 tacaacttaa gtaaataata attttgaata ataatttacg gaggtattca tatgtttaaa
    12301 cacgataaag cattattaaa agaagtaaaa gtagaaagac caaatcctca atatgctgtt
    12361 ttaatgcaag aacagttggg tggtgcaaat ggagagttaa aagcagctat gcaatacttg
    12421 tcccaaagtt ttagaataaa agaccctcaa ataaaagatt tatttcttga tatagcagca
    12481 gaagagctta gccatatgga aatggtagca caaactataa atttattaaa tggacatgat
    12541 gttgattata atgcagttaa tacaggcgaa atagaaacac atgtattaac tggtctatca
    12601 ccagtcttaa taaactcatc aggagctcct tggactgcta attacgttac agttacaggg
    12661 gatttagtag ctgacttact ttcaaatata gcttctgaac aaagagctaa ggtagtttat
    12721 gagtacttgt atagacaaat agatgataaa tatgtaaaag aaacaataga tttcttactt
    12781 aatagagaag aagcacataa tgctctattt agagatgctt taaataaagt taaagataca
    12841 ggttcaaata gagattttgg agttacagaa gattctaaat tatatttcga tttgtctact
    12901 ccagggccaa atcacgatac taagatagat attaatcctc cttcttttga aaaaccttta
    12961 aaaaaataat ttgatatttt ttatgttgtt ttgttttagt agttataaaa attatttact
    13021 agaagaatta attatatgaa aaattaaata tgtatagtta aaaatgggta tacacttatt
    13081 ataagtgtat atctatttgt ttattgcaaa acatataata aaataacaaa tgaggtggta
    13141 ttttggacag taatagaaaa cctaataacc taataaatga aaaatctcca tatcttttac
    13201 agcatgctta taatcctata aactggtata gttggaatga tgaggctttt aaaaaagcaa
    13261 aagaagagga taaaccaata tttttaagtg taggatattc aacatgtcat tggtgccatg
    13321 ttatggaaaa agaatctttt gaagatgaag aagttgctga aataatgaat agaaactttg
    13381 tggcaataaa agtagataaa gaggaaagac ctgatgtaga tagcgtgtat atgacagttt
    13441 gccaagctat gacaggtagt ggtggatggc caatgaccat aataatgaca ccagataaaa
    13501 aacctttttt tgcgggaaca tatttcccaa aatattcaag gtataatagg cctggagtta
    13561 tagatttatt agagaatgta tctgaaaagt ggaatacaag cagggatata ttaataaaat
    13621 ctggagatga aataatagaa gctcttaagg atgattttgg tgtaaaaaat actgagggtg
    13681 atttatcaaa agagatgttg agttcatcag ttagagtatt taaggctatt tatgatgaaa
    13741 agtatggtgg ttttggtaat gctcctaaat ttccaagtcc tcagaatctt atgtttttaa
    13801 tgaaatatta tagtatagaa aaagacaaag atgtgttaaa aatggtggaa aaaactttag
    13861 atggaatgta tagaggaggc ttgtttgatc atattggatt tgggttttca agatactcta
    13921 ctgataaaaa gtggttggct cctcattttg aaaaaatgct atatgataat gctatgctta
    13981 ctatagcttt tttagatgcg tataaaataa caaagaaaga gttgtataaa gaaattgcta
    14041 taaaaactat agattatgta gttagagaga tgaaggataa agaaggtggg ttttattcag
    14101 cacaggatgc tgatagtgaa ggtgaagaag gtaaatttta tacatttaat cctcttgaga
    14161 taattgaagt tttgggtgaa gaagatggaa ctttctttaa taattatttt gatataacta
    14221 gtagtggaaa ttttgaaggc aaaagtattc ctaatcttat aaaaaataaa gaatatgaga
    14281 gacataatga aaagatagct gaccttagta aaaaggtatt tgaatataga aaagaaagaa
    14341 catctttgca taaagatgac aagatactta catcatggaa tgcattgatg atagttgctt
    14401 taacaaaggc atatagtact ttaaaaaatg acatatattt agagtattca aataagtgtc
    14461 ttaactttat aaataataat cttgtaaatg agtcaggtag attattagct agatatagag
    14521 atggaagttc cgactattta gcatatttag atgattatgc ttttttaatc tgggcgtata
    14581 tagagcttta tgagtcaaca tttaatatga aatatttaga aaaggcttta aatttgaatg
    14641 aaagctgtat caaccttttt tgggattatg aaaaaagtgg attttatata tatggtaaag
    14701 atagtgaaaa tttaatagca agaccaaaag atttgtatga tggagctatt ccatcaggaa
    14761 attctgttca attatacaat cttataagac ttgcgaaaat tactggagat aatagacttg
    14821 aagaaatgtc atataaacaa ttaaaactgt atgtggataa tgtaaagagt tctccaactg
    14881 gctacagctt ttatatgctt tcattgatgt ttgaacttta ttctactaag gaaattatat
    14941 gtatttttaa ggaagattct gatttaattg catttaaaga attgataagt gaaaatttta
    15001 taccaaatgc aactttttta gcaaagaaat acaatgaaga aaacactatt ataggttttt
    15061 taaataacta tagattgaag gatgataaga ttagttatta cgtgtgtcaa agtaatagtt
    15121 gtagtcaacc aattaatgat ttacaaaaat taaaagatat gattttagga gtagaataat
    15181 cataatagcg aaaaaggaga tgctttaaaa taaaagtcta atcatctcct ttttttgtac
    15241 aaaaaacaga aaaagagttc ctaagaacac ctaatctatg taatgattgt tttgttaaaa
    15301 aataaaaatg acattatagt tatataaaat attgtactat aaaaaagtgg tattatagat
    15361 gcgatagtat taatcttttt tatttactct atttcttctt tttgcaaatt cttcaagtgc
    15421 ttcagattta attttatcta tttcttcata ttccataaaa ttagcatctt cttcaaagcc
    15481 atcttgttct tcaccattaa aacgatacat tttttttagt aaactccata aggtctctaa
    15541 ttcatctttg gtaaattcgt gaaatacatc tgccataaag tttattccag tcctactaca
    15601 tgtcaccata accttttttc ctaagtctgt aactttaaca ttaattgctc tcttatcatg
    15661 aggacttgga attacatcaa catatccatt tttttcaaga ttagcaacta gtctatttat
    15721 attttgttta cttgtaccca tttttcttgc aatattattt aatgttgttt cttcctctgg
    15781 taaatgaaga attgataaaa tagtcatata ctgtcttgaa gtaagtatac caaaatattt
    15841 atctccttct atttgtattt tgtttgtaag ggtaaaaaga gttgcatatg tttgattcat
    15901 taaaaagagc tcttttagtt catttgaata atccatgaaa tatatctcct ttatttttag
    15961 atgttgtttt gtcgaaaaca gaatatcatg aattaattct aatgtcaata tggtgtcaaa
    16021 atatattaaa gtattacttt tttatgtaac tattaattaa ttaaattatt gtataatttt
    16081 atgtaaaaac ttctatgtac caatgataac ttctatgagg aattaataat tcttaaattt
    16141 tttagaaaga ttttgacttg aaaatacttg taatataata tataaattaa tatactgaat
    16201 aaaatcaatc tggagggata attatgaagt gtacaaactt tactttcaaa ggagaggaag
    16261 gtttagatat atatacatat aaatgggaag atgaaaatat aaaaaaacca aaggcagtta
    16321 tccaaattgc acatggaatg gctgaaactg cacaaaggta tgagactttt gccaaggtac
    16381 ttactaaaaa tggttatata gtatatatca atgaccatag aggtcatggg aaaactgcaa
    16441 agataataga aaatgttgga catttagctg aaaaagaagg atttagatgt cttgtagagg
    16501 acatgtatac tttaacaaat attataaaga aagaaaatga agatctgcca atttatctat
    16561 ttggacacag catgggttca tttgcaagtc aaagatatat aatggattac agtaataatt
    16621 tatctggtct tatactatgt ggttcaaatg gaaagcaagg aatcattcta aatcttgctc
    16681 atctgataat caatcgtgag attaaaaagt atggaagacg ttctaaaagt aataaaataa
    16741 ataatttgat atttggcggc gaaataataa gaagaaatga aaagactaag tttgactggt
    16801 taagtagaga taaagaacaa gttgaaaaat atataaatga cccattttgt ggagtagtgt
    16861 gtagttgtgg ctttttttat gatttagttc aagggttaaa agaaattgag gataaagaaa
    16921 acttaaaaaa agtgccactt gatataccta tttacataat atctggagat aaagacccca
    16981 taggaaaaaa tggtaaagga gtcttaagac taagggatag atatataaaa ttaggagtaa
    17041 aagatgtaac ttgcaaactt tacaaagatg gaaggcatga attgttgaat gaaataaata
    17101 gagaagaagt ttttgaggat ataatttgtt ggttaaataa taaaatagaa atattgtaat
    17161 aaaaaaatat aatgatatat ttgctttaaa tttatttata tataatattt gaaaagtgga
    17221 ttatgtatat gcaaagtaga aaagagagcc cagaagtgat tttttgctat aatgccattt
    17281 attgaatagg tgggtgttat tataaataaa ttattaacac tcagtattat tatcattgta
    17341 tatggaattt attttattta aaattccttg aaagtttaaa agtttaaata tagtaagttg
    17401 taataaagag aaaatctaaa tatttaaatg ctatttaatg tgaatctatt aattaggtta
    17461 tacttaaact taattaatag attttatatt tcaagccaat gagatagggt tttagattaa
    17521 aatataacca atggttaaat tacagacatt ttgttaactt tccctcgatt tactagtaaa
    17581 tatttttttt atatacttaa attacaacag atgtactagg gaggtatata tgcaaattaa
    17641 aataggtaaa gtcatacagt gtcttagaaa agaacgaaat ttaactcaag aacaattagc
    17701 aaagtttata ggtgtgtcaa caccagcagt atctaagtgg gagagtggaa attcttaccc
    17761 agatatagaa ttattgcctt tattagcaga cttttttaat gtgtctattg ataagctttt
    17821 aaattacaag attgatttaa gtgaagaaga agtaatgaaa atttataaag aattagaatc
    17881 aggctttgct agaattgaaa ttgatttatc aacagaagaa ccaaaagaag aatttagaca
    17941 agatttagaa tctgtaaaaa agctttccaa tatgtatata gagaagtatc ctaaaagcta
    18001 tttattgaag ctaaggatat gttcgctgta tcagatgtat tcatataaat ttggtaagag
    18061 tgaacttaat gacagagtaa aagaaacaac gaatattctt gaagatatag tgagaaatac
    18121 agatgatatc caaataaaag aaacagctct tattatatta tcaaacgcat attgtatgtt
    18181 ggaggattat gaaaaggcag agttgtattt aaatatgata cataagtcaa taggggatac
    18241 aagtgttaat ttagctatga tatatttaaa gcaaaataga cttgaagaag cagaaatatt
    18301 gcttcaaaat aaattgttta gtaatgtatt taatataagc atggattgta agggaataat
    18361 taatgtatac aaaaatcaat acaaggaatt aaagaaaaaa ttggagaata gaaattttaa
    18421 taaaaatgca attgagagag aaatggagta tataaaaaat aagttgctgg ggtatgcaaa
    18481 tctttcttta gaaataaaaa aaatgctcag tgaagataaa ggtgcatttt ttagtatgta
    18541 tatggattat atggaattat ctctaatttt tttatttttt aacatgaaag aagaagcaaa
    18601 aaaagcttta tatagtctaa aagaaatatt agaaaaatat cctatacatg aaagtttgga
    18661 tgtaagtcaa atgagatttt ttgataaggt agagtctaaa aatttatata cttttaatat
    18721 atatacaaac ttgttaattg tacttaatga tgacagttat aatgagctta gagaagaacc
    18781 aatttttaag gatgtaattg aaaagatttt agatatggaa aaaatgttga aaaataaaga
    18841 ataagtaaaa atttaatata ggacttaaag attaatcaaa ttaaaagaaa gtgtttcaaa
    18901 ataaaaattt gaatcacctt ttttgtcggt cttaaacagt tatttagaaa aatagaggtt
    18961 tcaaaataga aatctgaatt atctgtatta ttacacaaaa atagaaacct aaatttataa
    19021 aaatagaata aagtttgtta tataaaatgt aattttgtct ataaaatctc acctgttttc
    19081 ttaactttaa gcgtgaaaaa gaggatgtat catgaatttt ttagttcatg atgcatcctg
    19141 ctttgtatga aatccaatat atctttatta tttaaacaat ggaaaagagt cttatttata
    19201 tttggagaca tctcttttat ttaattatga agttttatac agttcaataa ataggaatta
    19261 tatttagaaa ctcatgacat tatttcttag atagtgatta aatttatcat attccatttt
    19321 agataacttt tctgggtaag acattacaat caggttatgc ccattaaatt ttttatggta
    19381 tggtggatga atgtgttcat aattatttgt cttatatttt actctttcat agaacgcctt
    19441 gcgataaatt gaaatctcat caattggaag gtcaatttct aagataatag tttttccttt
    19501 cttatttaaa agctctaacg ctctttgccc atatctatta ttgcgcattt ctggaaaaat
    19561 gcagaaatgt tctacataga taaagtcatc tgtttcccaa cataaaatta tgccgacaaa
    19621 ctggttttta tcataaatta aattgaattg atattcttta tttttcaaaa tttctgcttg
    19681 caaagaagat tttctttgtt catgaaatgg gaaactaatt ttatataatt ccatagcctt
    19741 gtaatataat ttattttcac tggttcttaa ccattcaaat ttcactttac tcctcctaat
    19801 cacgaaaatt ccaatttatt tatgagttca ttttaccata gtcagtattt gcatacaatg
    19861 tgttttaagt tgagtgtcat ttctttcttt tatttatgct tatatctcat agtctaagag
    19921 ataattgctt aagacataac taaaaactat tatattaagc tttgataatc taaaaacagt
    19981 atatttatgg acttttctag gtctaaaagt aatcttaata attttagaag ttacaaaaaa
    20041 gttacataaa tttggaaagc tataatatac atatataata taaggttaaa actgtaatga
    20101 ataatattaa tttaaaattt gaacatggaa ggagattatt atggagaaaa gagaaaagag
    20161 gataataata tcatctttat taagtgtatc aattttaatg gggttggtaa gtatatattc
    20221 tatattaaat aaagaagaca taattttaac tgttaagggt caagagcaaa aagtatcttc
    20281 ctttaagaaa acagttgagg aacttttgga tgaacagggt gtaaagtata attctgaaga
    20341 taagattaat ccaagtttag atacagaact aaaagatgat atgaaaataa aagttgttaa
    20401 ggtaactaaa agtaaaaaag aagagattga aaaaattcca tttgatacaa agcatgtaaa
    20461 tgatagtaat ttgttaaaag gaaaatctaa agtttatcaa gaaggtcaag aaggagaaaa
    20521 aaaactagtc tataatttaa cttaccatga tggaaagtta gtcaaaaaag tcttatcaaa
    20581 agaagtaata tctaaggagc caactacaaa aattataaaa tatggaacta aagaaaaagt
    20641 actaatagca tcaagaggag caaatataag aggaggcaaa catatgaaag tggttgccac
    20701 tgcatatgca ggagatacaa taacatctac tggtacaact ccaagatggg gtgttattgc
    20761 agttgaccca cgtgtaatac catatggaac aaaagtatat atacctaaac tgggtatgac
    20821 ttttgtagca gaagattgtg gaggtgcaat taaaggtaat agaatagaca tttttatgaa
    20881 cagtgaagga aaagcttcaa attggggaag gaaaagtata gatatatatc tacattaaat
    20941 tagtgaatag aataacttta cttgaaggac aaaaaagtat aaaatatgaa ttaactaaac
    21001 tgttagatta ttcttaaaac ataaaaatat atctaagggg ggataagatg aaaatagtct
    21061 gcttaggaga cagtttaacg tatggatttg gagtatcacg aagtaattca tggacaaaca
    21121 ttgtaaataa agagactcga ttagaaatag taaataaggg tataaatggt gatacaacaa
    21181 gtggtatgtt agctaggttt aatgaagatg ttgtaaaaaa ttctcctgat atagttttta
    21241 taatgggtgg caccaatgat tttattgctg gtgcgggaaa tgaaattatt aattctaata
    21301 taatggctat ggtgcatcag tcatattcaa aaaatattat acctataata ggtataccat
    21361 taaaaccaga tattccaaat gttagggaag attggagttg ttttactgat tttaatatag
    21421 tatctcaaaa cttagaatca tatagttatt ggataaaaaa attttgtatg acatttaata
    21481 ctaatttcgt agatttttat tcagaatata ataaaaatat ggaactagaa ggatataaaa
    21541 aattatattt tgatgggtta catccaacca aagagggtca tagaataatg gcagacattt
    21601 ttattaattc aattaataag tatatagaag agttatagat tacattaaaa tgcttgttta
    21661 tgcgtcagtt gcaataaata catacagtag agaggggttt attatggatt atactaaaac
    21721 ttatgaagag tggataaagg gttcatattt tgatgaagat acaaagctag agttggagaa
    21781 tataaaaaat aatgaaaaag aaatagagga tagattctat aaggatttag aatttgggac
    21841 tgcgggtctt agaggaataa ttgaagcagg aacgaataga ataaataagt acacagttag
    21901 aagagctact tttggattgg caaactatat attagaaaac acaacaaaag aagaaacaag
    21961 tagaggagta gtaatagctc atgataatag acacaagtct agacaattct gtatagaatc
    22021 tgcaaatact ttggcagcat gtggtataaa agcatatata tttgatagtt tgagaactac
    22081 tccagaatta tcttttgctg ttagaagctt aaatgcaatc gctggtatag tcataacagc
    22141 aagccataat cctccagaat ataatggata taaggtatac tgggaagatg gagcacaagt
    22201 tatgccagag attgcaaatt caataacaga gaaagtaaat agtatacatg attatagtac
    22261 aataccaacc ttaacggaag ctaataaaaa tttggtagtt ttgttggacg aatcacaaga
    22321 tactaaattt atagaagcag taaaaagcca aattataaga aaagatttag ttaaaaatgt
    22381 aggtaaaagc tttaagatag tttatacacc tctttgtgga acagggaatg ttccaataag
    22441 aagagcattg aaagaagttg gttttgaaaa tataattgta gttccagaag aagaaaatcc
    22501 agacccaaat tttgcagggc ttgattatcc aaatccagaa gagaaaaaag ctttaaatag
    22561 aggtatatta ctcgcaaaag aaaaaggagc agaccttgta atagcaactg acccagattg
    22621 tgatagagta ggtgtagcag taaaaactac tacaggtgag tatgctttac ttacaggaaa
    22681 tcaaattggt ggtatgctga cacattatat aatagaagga ttaaaagaaa ataataaatt
    22741 aaaagaaaat cctacaataa taaaaaccat agttacatca gaatttggag cagatatagc
    22801 taaagcaaat aatgttgatg tattaaatgt tcttacaggt tttaaattta ttggtgaaaa
    22861 aataaaatta tttgaacaaa ataaaaatag aagttatgta tttggatatg aagaaagtta
    22921 tggttactta gttgggactc atgctagaga taaagatggt gtagtttctt cattgttaat
    22981 atctgaaatg gcagcatttt actattcaaa aggtatgagt ttatatgatg gtttaataga
    23041 actttataaa aagtatggat ttttcaaaga acagactata tctttgactt taaaaggtat
    23101 agaaggtgta gaaaagataa aagaaataat atcttatttt agagaaaatc aaattgatta
    23161 tataaatagt attaaagtag ttgataaaaa agattataaa aatggtatag ataatcttcc
    23221 taaatctgat gttttaaaat actttttgga agatgaatct tgggtagcta taagaccatc
    23281 tggaacagaa ccaaaactta aattttacat agctgtaaaa ggagctagtg atattgaagc
    23341 agataaaaag attcaaggtt tgaaaaagta tatagatgac atggtcgaaa aattaaaata
    23401 aaatattatt attttataag ataattttgt aataatattt atttagctga aaattataat
    23461 ttactttcat tgagtagaat atggaaatgt tagtagaatt gttattttca gcttttattt
    23521 gtgttatttt tacttaaata attatattat agaatgaatc tgagtataat taatattgta
    23581 taatgaaatt aatttgatag aaaaggggat atttatgttt ggtatagata agaaattgat
    23641 tgaatatata attgcaaaat ccaattacag tgaattaagt tcaaagtcag cagaagtatc
    23701 tttcttctta atgttgtcca tatttccttt tttaatattt acaataagtt ctatagccta
    23761 tataccgatt cttcatttaa ataagtatat agccttgttt agaaatatga tgccagaagg
    23821 tgcttttgct gtgttatctt caatcatagt ttctgcaata gacaatagaa acctgaaatt
    23881 tttagcagtc agttttgtac ttactatgtg gacattttca agagcagtaa aagctcttat
    23941 taaaggaatg aatagagcct ataaagttaa agaaactagg tcttttttta aaattttatc
    24001 tatatcattt ttatttacaa taatgctttt ggtccttatt tttttatcta tgatattttt
    24061 agtttacggg gaaaaaatag gttattttat ttttaatctt gtaggattgg atgagatatt
    24121 tataaaaata tgggatattc taagatacac tgtaggtata ataacaataa tagtgatatt
    24181 tacactttta tataaataca ctccaaataa aaagttaact attaaagaat ctgcgccagg
    24241 tgctattttt gctacttttg cttggttttt agtatcattt ttatattctt attatacaaa
    24301 ttactatgct aactatgaag taatctatgg aagtatagct gaaataattg tacttatgac
    24361 ctggatgtat tttagtagtt ggtctatcgt tattggctat gaggtaaatt caagattgta
    24421 ttttagaaag ataagacatg aaatgttaaa gtaaagtatt taaaataaaa gagagtattt
    24481 agtattataa atgtaattga attaataaag aaatattgac atttaagtta aaaagtctag
    24541 aatttttctg ggcttttttt atttttagta taacttgaca aattttattt aatgggatag
    24601 ttttaattta taatcatctt taaaaagatg actattgata agtaagagat aaaattggta
    24661 agtatcaaaa aatatactaa ataaaaatta tagtcctaat gagttaattt ctgtattttc
    24721 aaataatgca gtatttagtc atgtgttttt atatgtgcct gtatttctaa ttaccaatat
    24781 attagaataa gctactgtaa ttaggtttag aaaaaagcga cataaataat tataaacaaa
    24841 taataaatta cataaaatta aatttattca caagtatatc ttataaaata tgttataatt
    24901 aatagaaaat tgtcatataa ttaattaagt agtattatta taaataacat tgaaaggtag
    24961 tggttagaat atggataccc ataataaata tgtaaatttt ataaaaaata tacctgtacc
    25021 ttttttatat tgtagaattg taaagagaca agaagatata gaatatcgag tagaatatat
    25081 aagtaagggt atgggcaaag tattacaatt agaagaaggt atttgtgata aaaatatatt
    25141 agatgtattg cctgtattta agtctaaaaa atatttcaaa gagttgttct caaatgaagt
    25201 ggattgtata aaaaggtaca ttccgacact taaaaactgg ataaatataa aaaaacagat
    25261 aataggggat tcatacataa ttttgtattt tggtaaaatt gtatttgatt atagacagat
    25321 aatcgattcc tttgataaaa aggaaaaagt agcttatatt aaagacgaag aaggcatata
    25381 tatagattgt agtgaaaatt taataccaat attaaataac aacattaaaa caacaaaaga
    25441 tatctttggt aaaaatgata tagaagtatg gggagagaat acaggaaaat tatttagaga
    25501 tgattataga gaaggagtat ctagtaaaaa aagattttta caaaatcttt ttgaatatga
    25561 agaaacattt tttatggttg aaaaatattt tttgtatgat gaagacgaac ttttaggtac
    25621 tatagggata gtagacaata taatatatag tggttacagt aatagaaatt ataactcaaa
    25681 agatttaatg aagatgatag aacactcaat tccagagaat atgttttata aagatgtgta
    25741 tggaaattat attggattta attcaggttt tttgaattta gcttgtatga ataaagaaga
    25801 attattaggg aagaatagtt ataaaatatc agaagaagaa gctttaatag ataagatatt
    25861 tgaaagtgat aagggtgtag ttgaaaataa aaaagtagtt acatttgagt taaatataag
    25921 tatgaatgat gaaaacaagt gtattgagat tacaaaaagg ccgttttttg atagctatgg
    25981 aagtgttatt ggaataattg gaacagcaag ggatataagt agaagaaaaa gattagaaga
    26041 ggaaatggat aaaactagga tggaattttt tgcaaattta tcccatgaac ttagaacgcc
    26101 tataaattta atatcatctt cattacaagt gatagaaaaa aaagaagcag atttaataga
    26161 atcaaatgat actttaaaaa gaaatctagg tataataaaa caaaatggaa atagaatatt
    26221 gaggttagta aataatgtta tagactttac aaaaatgcaa tcaggatatt tagattttaa
    26281 accagaagaa tctgatataa ttgcatttat agaggagata tgtatgtctg tagctgattt
    26341 tgcaagccaa aataatatac aacttacatt tgatacagaa atagaagaat tttcaatgtt
    26401 atttgattca gaaaaattgg aaagaataat attaaattta ctttctaatg gaattaagta
    26461 caataagaaa gatggaaaaa taaatatatt tttatatgtg aaagataatg tatttaatat
    26521 gaaaatatct gatagtggaa taggaatacc aaaagaaaaa atagataaaa tatttaatag
    26581 gtttgaacag attgacaatg aactttctta cagagttaaa ggaagtggta ttggactttc
    26641 attagtgaaa tctttggttg agttgcatga gggaagtata tctttaaaaa gtcaacttgg
    26701 tattggaagt gaatttatag tttcattgcc tgttagaagt aaaaataata ttgaaaaata
    26761 taatcataaa agagaaatca gcaatgaact aagtaaaaaa ttagaaatag agttttcgga
    26821 tttataagtt taaatataaa aaataaaatt ttaaaaaagt attgacaatt gatttagaag
    26881 ggtttaaaat aaattacaac ataataaata aaaaggcgat gaataggaag agtaaatcta
    26941 attcgattac cagagagaag agtccattgg ctggaagact ttttaaatta gagggattga
    27001 aaactaccta ggagctactt tctgaagtca tctgcattag tagattttag taagaatagt
    27061 cgttagctgc gttaaagtgt aaagtgagtt ttcatgacat atgaaaacta aattaggtgg
    27121 aaccgcggga ataatactct cgtccttatg ttataaggat gagagttttt tgttatttaa
    27181 aaatataaac agtaatcata ataattatag aaaatataaa agcgatgaat aggaagagta
    27241 aatttaattc gactatcaga gaggaaagtt cacaggctga aagactttct aagtttgaga
    27301 aattgaaaac tacctaggag ctgtggtctg aagttacttt aaaaagtttt agtaagacta
    27361 accgttaact gcgttaaagt gtaaagtgga ttttatcaat aaatgataaa atcaaattgg
    27421 gtggaaccgc gagtatggac tcgtcccttt atatagggat gagtctattt ttattgcaat
    27481 aaaagattga aaataaaaaa aaatcggaaa attataaatt taggaggaaa ataaaatgga
    27541 gaataaagtg aaaattggag tactaggata tggagttgta ggaagtggac ttatagatat
    27601 aatagataat aataaagaaa aaagaaatat agaaatagtt ggaattttag ttaataattt
    27661 ggaaaaacat aaggataaaa aatattctaa tataataact aataatatag atgatatatt
    27721 taataaagat atagatatac ttgttgaagt catgggtggt ttagaaccat cattatctta
    27781 tataaaaaaa gcattaaaca ataaaattca tgttgttaca gcaaataaag atttgttagc
    27841 tgaatgtgga gatgagcttg caaaattagc aagtgaaaat aaagtaagta ttaaatttga
    27901 agcttcagtt gcaggaggta taccagtatt aaaaccaata atagaatcac tagaaggaaa
    27961 taatatagat agtataaatg caattttaaa tggaactact aattttatat tatctaaaat
    28021 gtatgatgag aatctatctt atgatatggc attaagacaa gcacaagaat taggttttgc
    28081 tgaagcaaat ccagagtcag atgtattagg gtatgatgct gctagaaaac tatcaatatt
    28141 atcaactcta gcttatgaca atagagtata ttggaaagat gtgtatttag aaggaattac
    28201 agatatagat gaaaaagata ttgaatatgc aaaaaaatta aattgtaaaa taaaattgat
    28261 tggtcaaagt aagtatgaaa atgataaagt aagtgctttt gttagacctg ttttagtaga
    28321 aaaagataat atccttgcta gaatagacaa tgagttcaat gctgtcatag tgaatggaga
    28381 ttcagtagga gaagtttctt ttgtaggtaa aggagctgga agtttagcaa caggaagtgc
    28441 ggtttattca gatgttatag atataataga taatagagtt tcaagtatag attcttttac
    28501 taaagataaa atacaagtta ataaaatagt tagagaaaag tgtggagcac ttcttagatt
    28561 taaaaaatgt aataaagatg aaattctaaa tatagttgag aattgtttag taaaattcga
    28621 tattttaaat gatgatgatg aacttgctat tatggtttat gctgattctg aatatgaaat
    28681 aaacaacagt ttatgtctaa taaaagataa aggatactgc gaaaaaatga ataaaatgtt
    28741 gaaaataagt taaaaaagaa aaaatataag tttatatgta acatattttg acgattttaa
    28801 tactatatag tatgagatga aacaaaacat ctcataaaaa aattaaaatt aaagggagag
    28861 atatgttata tggaaaataa aaaatgttat tcagaagatt ggtatgaaag aggagaatct
    28921 acagctaaat ggttccaaaa tgatagagaa gaatatgaaa gagaagcata tgatgaagat
    28981 agagaaagaa gaggttcaaa ctgtggatgt tcagattcag gagaaaatag acctagaaac
    29041 tgtgaaagat ttagaagaga agctgagata agagaaagag aagcaagaga agcattttgt
    29101 gaatcttcag agaaaaagaa agaggcatta gcatatgaat gtgaagctag aaaattatgg
    29161 gaagaagcag aaaaatactg ggatgaatat tcaaaataca actataaagg aatcgaatat
    29221 ttagcagaag ctgctagatt atttgatgaa ggtatggaat gtgaagctag aagaaatgga
    29281 aataatggag gaaacaataa taattgttgc cataaatgcc ataaatgtaa ttgtaactgc
    29341 tgtagaaaat aaaaaaatta attgacaaat caatagttaa aatatatcat attatgtata
    29401 ataaaaagaa aattcagaaa aaaataatga ttagaagaaa tataagtata tttattagac
    29461 aaatagtgct cagttagtga ggtgagtaca atatgattaa actcccatcc tctagctttt
    29521 gatagagggt gggagttttt atttattgat atatcttaaa tgaggataat attgtaaaat
    29581 aaatgagtat gagattgtaa aattaggagg taaaaaatga aaagtattaa aacatcaagt
    29641 atgaaagaaa aggtagatga aaagatatta agagagcaag taagttctat aatagaagat
    29701 ataagaaata ataaggatgt tgcacttaaa aaatataatg agaagtttga tagaaataca
    29761 agagatgaat ttagaattac taaggaagaa ataaaggaag cttataagca tgtagatgac
    29821 gaatttataa ataacttaaa gattgctgcc aaaaatataa gagaatttgc taaagctcaa
    29881 aaactaagtt ttgaaaaccc ttttgaaaaa gaaatttatc caggtgtaat cttaggtcaa
    29941 actaatattc caatagagtc atgtcttgca tatgttccag gaggtcagta tccattgttt
    30001 tcaacagctc ttatgcttat aatacctgca aaagtagcag gagtaaaaag aatagtggca
    30061 tgttcaccaa ctatgaaaaa tactgaaaag ataaatccta agacattggt agctatggat
    30121 atagcaggtg ctgatgaaat atatgcaact ggaggagttc aagctatagc agcatttaca
    30181 tatggaacag aaaagattaa tccagttgac ataattgtag gaccaggaaa taagtttgta
    30241 actgaagcta aaagacaatg ttatggacaa gtagggattg attttgtcgc tgggccaagt
    30301 gaagttctta taatagcgga tgaaacttca aatccagttt atattgcagc tgatttatta
    30361 gcacaatgtg agcatgattt aaatgcaaga ggaatattat tgacaaatag cttggaagta
    30421 gcacaaaaag ttgaaaaaaa tatagaaagt atgctaaaag atttgccaac aaaggacata
    30481 gcatatagtt catgggaaaa taatggagag ataatattgg ttgatgatat ggaagaagcc
    30541 attaaaataa gtaattttta tgcaccagaa catttagaag ttgcagtaaa tgagtataat
    30601 gatatttgtg atagattaac taactatggt tctttattta ttggaaatct ttctgctgag
    30661 gtgtttggtg actatgtttc aggtaccaat catacattac caacacttaa agcatcaaga
    30721 tatactggtg gtgtttgggt tggaactttt atcaaaactt gtacaaagca gatatttaat
    30781 gaaaaagcta tacaatcatt agcacctgta gctgaaaagc tggcaaaaga agagggatta
    30841 tatgcacatg caaaggctgc tgaggttagg tttaaaaagt agtaaaaaat tttattttgg
    30901 cagtcgagac ttagtattag actgagataa ggaaaacgct gagaacctga ataaatacta
    30961 tattttgtgg gtctcggcgc ttcttttata tctgtatata atagaataat actatctgtg
    31021 tgtttaaata ctaacttaat atttcttttc taaataatgt ttatgaaata gttaatattt
    31081 ttagttaata cttgaaaaaa acttttgcta actgtgcaaa ttcatctgca tttgtcaata
    31141 tagcagggtg tcctccctga ctaaaaatgt aaatacttgc ttttgaaata aaagttgcca
    31201 tttgcttgta ctcttcttca agattatgac gacacatttc gtcttcctta cttcctgtaa
    31261 ataaaattgg catttctaat ttacacaatt ctttatgaaa caaagggcgt ttctcgtttg
    31321 cacattgcaa tagagctttt gtatcaagat taacaacatt ctcccaatct tttccatgac
    31381 accattcata gaattttcgt gcttgaatat cttgctttga tttttccctt ccagtgatta
    31441 gattatctat gaagttatca ttaagagtac gtccatcaaa gctatctgct ataattgctt
    31501 caactaaatc gggacgttct aatccagtat taacagctgc ccatgctcct ccacttgttc
    31561 caattaagcc aactttacca caattcaaat gttcaatcag tgctattgtc tgtaaggctt
    31621 catcatgcca tatgtcagga gaaaattgag aaactctatc cgactgtcca ttacctaaaa
    31681 aatcaattaa gatacatcta aaattttctg catataatgg cacaagtgct ttaaacatat
    31741 tagaagaacc agtattacca tgcaggaaaa ttatcggttt accttgtcca tattctttat
    31801 aaaaacaaga attaccttga tactgaaaat atgacatata ttttcctcct ttttatttgt
    31861 taagttaatt ataacaatat aggtatgaat gaaaaacctc atgttctttc ttagaataaa
    31921 aatgtgccac gcataaaacg tgacacattt ctaattataa attttgctgt ttaagtattc
    31981 tttgtatgct tttctctgtc aaaaagtatt tctctgataa ctgcttgata ctcattccat
    32041 tttggaaatt tatacagatt tgagaattac gagaagctaa gacttgtttg gtgtttgtgt
    32101 tttcacccca atgctttttg tgctcttgct ttttgggaat ataaatcact ttaccatcta
    32161 tatattcttg cacttgctca atcagttctt taggcagtaa gtcaatcgaa ttttgatagc
    32221 tcattttgca cctcctaaat aattattctt taggattagc aaaggctact gccactcaca
    32281 attaaaaata aaatttttta ttatgtacaa tagcctttgc tatgcacaag ttccaattcc
    32341 cataaactaa atactccttt ctggtttgaa aaattcatct gaacattgat ttcgctttta
    32401 agatagagtt caatgacaaa tttacatttt tattatatat attcagatag ataattgcaa
    32461 ctgtctttgt atgaggatat tttattatag tcaatactat aatatgtatt tatatcatca
    32521 aactaatatg aacagttgaa aggaatatga acaataaaac ttagtcagga agaataatta
    32581 tgaagcagac aatagagaaa tttgatttta aagttttatt gaaaatttat tgacaaattt
    32641 aacttgtaaa tattatacta aacttaataa ataaagttta taaagtaagg tgttgtctat
    32701 gaaaaatata gttggaaaac cgcaaacgat aaaaaaagta aatgaagatt taataaaaaa
    32761 cattataaaa aatgaaggtc ctataacaaa accagaaatt tcaagcatta ctgaattgag
    32821 tttagctaca gttaataaga ttgtagagca attagctttg aaaaatgaag ttaaagtaag
    32881 tggactaagt gaatctactg gaggaagaag agctcaatta tttgaaataa atgcaaactt
    32941 agaacacata atagctttat attattatag aaattgttgt ataggggtag ttgccaattt
    33001 attaggtgaa ataatttatc aagaagaatt taatataaga atggatacgt atgaaaacgt
    33061 taaccaagat actttttttg ttatagatac tctaataaaa aaggaaaaag gaaacaatat
    33121 aagggctatt ggaattggtg tcccaggagt tgtaaaaaaa ggtgtagtta aaaatatacc
    33181 aactataaaa agttgggaag gtataaattt agaaaattta ataaagagta aatacaatac
    33241 taaagtgttt atagaaaatg atacaaatat tactactatt gggatttatc aaaaacagta
    33301 taaagacaaa tataaaaata tggctcttat gtatttagaa gatggtattg gttcaggtat
    33361 agttataaat ggtgaattgt atttaggttc tacaaatttt gctggagaat taagttatct
    33421 aaatatagaa tttggtaagt ataactctaa caacttaaat cttgaagatt atattatgag
    33481 tttaagaaaa agatatttag aaagtaaaga taataaattt aagaaagaaa ttttatctat
    33541 aatatcaaac tttataagga ctcttgtatg tgtcctaaat ccacaagcta tgattataca
    33601 gtgtagtata ttaaccaaga gtgacatgga gtttatagaa aaagatgtaa gcaataatgt
    33661 tggagaagaa aattttccta aattaataaa agtagactca ttgagagaag gaagtataaa
    33721 tggagtaatt agtatgtgtt taaaagaaac tgactatcaa tattccttat ctaataaaaa
    33781 aggaggatat taatgtgaaa tatttggttt caatagatgg agggggaaca aaaactaaat
    33841 tttgtgtaag tgacttagat ggaaacatac ttaaagaaca taccactggt tcaactaatt
    33901 ataagtcagt tggaattaaa aaaacatatg aaaatattaa taatggattt aaaaaaatat
    33961 taaaagattt atatatcgat tatgacgata ttgagtacac tgtatttggt atatctggtt
    34021 gtgattctcc taatgattat aagattataa tggatgaaat attaaaaatt gggattaaca
    34081 aagaaaaaat atatctggca aatgatgcag ttttagcttt ttatgcacaa gctgattccc
    34141 ctggcttagt aattgtggct gggacaggtt caattatact gggaattaaa gaagatggag
    34201 aaatatatag agttggtgga tgggggtata attttagtga tttgggttct ggatatgata
    34261 tagggagaaa attattaaag aaagtattgc tttattgtga tgaatgtcat gagtatagtg
    34321 atttatttag ttgtgtcttg gacttctttg gagcaaatag ttttgaacaa cttgcatata
    34381 tgattactga tataaatgat aatgttgaaa tagcaaactt agcttcatta gtgattgatt
    34441 gtgccgaaca aggtgataaa ttggctattg aaatattaag agaatcatca actgaactat
    34501 ctaaattagc tcaagtaata ttgagaaaaa tatctaatcc aatgaaaatg gataaaagcg
    34561 aaataaatat agttttgtct ggtggtacat taaataaaac tgtatatgct caaatgttaa
    34621 tgaataattt aaaaacagaa aatacagata aaaatttaaa atttatatct caagaaaatc
    34681 agccagtata tggggggata agacttgcta tggcattagc taataggagg gtcaagcatg
    34741 gatagaaaaa aggtaatagt acaaattttt actggaggtt ttaaggctaa agaaataaag
    34801 tttaatgata taaaatgtaa actactagaa ttattggact gtattgatat agaaaaggtt
    34861 atcatgggat ggagtgtaga tgaagaatta tatgtagaaa ctaaaaaact attaaataaa
    34921 tataatgttg aactatatct ttggttgcca tgtttttctg aaataggatt acttgaaaaa
    34981 agtaatttac taattgatta tatggggaag gaagtcaagg gctatgcttt acaagaggat
    35041 gaaaattttg agttttattg cccaaataat aaggaaaata taaaaaatat aaagaaaata
    35101 tatagtactt attttagtga tattaaattt gatggaattt ttttagataa aataaggtat
    35161 ggttcttttt cgaataaact aaatggtgtt tttaattgtt tttgtaaaga ttgtttaaat
    35221 atatataaaa aaaataatat tgataaagag tcgttaatga gagaaatgaa taatgtgtct
    35281 aagggagtta tagataatga agatactcca tttggtataa cagaatacat taatggaaaa
    35341 tacaaattta aaaataaaat atgggaagat ttttttgaaa caaaaaatca aaatatatac
    35401 aaggcattat tagagataag tagttatttt aaaagtaagg gattgaaaat aggtatagat
    35461 gctttttctc cttttatttc ttactttaca ggtcaagatt tagaaaaatt acaagaagta
    35521 gcagatttta taaaaccaat gatgtataga actacaaaag ctccagctgg tttaccattt
    35581 gaatttgaaa ggtttattga tgaatccata cataagaata aagagattgg aaaaagaaat
    35641 ttcttagata ttttaggaat taaggagtat gaaggagatg tatatcctat agaatttgta
    35701 ctagaggaaa tcgattttat gacaagaaat cttaataaag atatatatgt aggagttgaa
    35761 ataaataaaa aggaaattat atctcctgtt acacctaagt acataagtga aaatatttca
    35821 aatataaata agacaacagt aaaaggatat gttttatctt gggatttgtt gtcagcacca
    35881 aaagaaaata ttgatgaaat tataaaatgg tttcggaatt agtgaggtga taaagtgagt
    35941 aagatattgg aaatacaaca aatatataaa agttttggaa aggtagatgt tttaaaaggg
    36001 ataaattttg agttagaaaa gggagaagta agagctcttt tgggagctaa tggagcagga
    36061 aaatctacac ttattaaaat tttaggagga gttcattctc aaactaaagg taaagtaatt
    36121 ttgactggta atgaaataat gtttaaaaat ccacatcatt ctaaagaaaa tggtattagt
    36181 ataatttatc aagaattaag cttgatacca acgcttacag tacttgaaaa tatttttctt
    36241 ggtagagaaa ttttaaaagg aagtgtgttt ttaaacaaga aagacatgct tgaggaatat
    36301 atgaaaattt gtgaggattt tgattttaat atagacccac atatagtagt ttctaattta
    36361 agtgtagcaa accaacaaat ggttgagata atgaaagctg tttcatgtaa ttctgaaatt
    36421 atcataatgg atgaacctac aacatcactt actaataatg aaaaagatgg gctatttaaa
    36481 ataataagta gactaaagtc taaaggtaaa acaatagtct atatttctca catattagag
    36541 gaagttttta aagtatgtga tagtgcaagt ataatgaaaa atggaattat gattggaact
    36601 tatgatgtag aggatttaac taaattaaag ataactcaac ttatgacagg aaatgataat
    36661 cacaaactta ttgagaagaa ggattataca tttgcaaaat atgaagagcc accagtacta
    36721 gaagtaaaga atataagctg gtctaacaaa ataaaaaatg tttcatttaa agtttataga
    36781 ggcgaagttg taggtcttgc aggtcttgta ggttcaagaa gaactgaaat tataaatttg
    36841 atatttggta tagataataa agatagtgga gatattttta tcaatggtaa aaaagtagac
    36901 ataacttctc ctaaggttgc tataaaaaac aaaataggat tgattcctga agatagaaaa
    36961 aatctaggat taattttaga acaagctata tacacaaact catcatcagt acaaattgat
    37021 aaattcaaga aatttttgtt tatagataga aataaagaaa ttgagttttc taaaaaaggt
    37081 gttaaggaat taggaataaa agttagtgat gtaaagcaag aagtaaaaga gcttagtggt
    37141 ggaaatcaac aaaaagtagt agtttctaag tggttagaga aagatttaga tttaataata
    37201 tatgatgagc cgacaaaggg aatagatatt gctgcaaaag aagatatttt caatacaatc
    37261 aaagaatttt caagtaaagg aataggagta atatttatat cttctgattt agaagaagta
    37321 ataagagtaa gtgatagaat tttagtaact agagaaggtt ctattgtaga tgatttaaaa
    37381 aatgagaata tacaggttca agatataatg aacagtatat ttaatgtata aaaaatgaaa
    37441 atggaggact gagatgaaga aagcttttga gaagttaaat ttaagagatt atggagtatt
    37501 attaggtttt ttagctttat gtatagctat aagtattgca actccatcat ttttaggtaa
    37561 acaaaatata cttaatcttt tacgacaatc ttctataatt ggaataattt cagcaggtat
    37621 gacttttgtt ataatatctg gaaactttga tatatcagtt ggagcagtag cagctcttgc
    37681 aggtgctata acaatgaaat ttgcgactgg tggaactaat ttgtttgcat ccatgttctt
    37741 aggtgtagcg atttgtgcag taataggatt aatcaatggt atagtagttg caaagataaa
    37801 tgtaccatca ttaatagcca ctatggctat ggtcactatt gtaagaggat tgctgttgat
    37861 gcttacagga ggatatccaa ttactgaaaa tataccaata cttgattaca ttggaaatgg
    37921 atatttgttt aatataccaa taccagtagt aattttcttt atagtggtgt tagcttcgtt
    37981 tattgtactt aataaaacta agtttggtag atatgtttat tctgtaggag ggaatcaaga
    38041 agcaagtaaa cttaatggta taaatgtaga ttctcacaaa gtgaaagtgt ttattattaa
    38101 tgctgtactt gcaggaatag ctggaatcgt actaacagga agacttggga ctgcaactgc
    38161 gattgcaggt gaaggatatg atatggatgc aattgcatct gtagtaatag gaggtacatc
    38221 agtggcagga ggttctggtt ctgtacttaa aactgttata ggagtattac ttatgagtgt
    38281 tataaacaac agttttaatc ttcttggaat agatgtatat ttccagtaca tatttaaagg
    38341 attaataatt ttagcagcag ttggatttga ttcttatagc aagaaaaaac ttgcttcaag
    38401 ataaaaaata agggggattt ttttatgaaa aaattaatga aacatttagc attgttgcta
    38461 tcacttgtta tgatatttgg tttagttggt tgttcaaatg gtggtgacaa agaaaaaaat
    38521 ggagaaaaga aaatagcagt attactaccg ggttcaacag gttactttgt agcaactaaa
    38581 caaggtatag atgcaaaatc taaagaatta ggtgtaagcg tggaatatgc agatgctcaa
    38641 tgggatgcta gtaaacaatt atcacaagct gaagatttta tggctaaagg ggtagacatg
    38701 attgcattat gtggagttga ttctgctgta agtgagagaa tagttaaagc tgctaatgat
    38761 tcagacgttc ctatagtagc atttacaaat tctataggaa gtaacccaac tggagaattt
    38821 aaaggtttag ttacatatat aggacaaaat gaggaagaaa caggagcatt aactggaaaa
    38881 attgctaaaa acttattagg agaaactggt gggaaagctg tattaataga aggagtacca
    38941 ggaactactc cacaagtatt aagaaaaaaa ggtcttgaaa aagaattaaa agatagtaat
    39001 atagaaatag tttataatca aacttcaaat tgggagaaag agcaagcttt aaaggtaaca
    39061 gaagatttaa ttcaaaagaa aactgatttt aatgttataa tatgtcaaga tgataattct
    39121 gctacaggtg caggtcaagc tttaaaagat gctggtctta aggataaagt taaagtcata
    39181 ggtttgggtg gaagtaaaga tggattaaaa gctataactg atgggttaat agatggtaca
    39241 acttatatgt ctgctgtaga agaaggtgga ttagtcatag aaaaggcatc taaattcctt
    39301 aaaggtgaaa aaatagaacc tgttacacaa attaaacaag ttgaagttaa caaagataat
    39361 atttcagagt tcaagggtga atggtaaatt gtcatagata ataaataaga tttttagagt
    39421 tactttagaa actatataaa gtaactcttt ttttaattta atttagtgat aaattattga
    39481 atagtatttc taaacaaata aaaaattaat ataaacaaag acttaattat actaaattat
    39541 aagtaaaact aatataaact tatggaaaaa aggaaaacgt ctaaagtatt ggaattacta
    39601 tagtgtaggt gtggtattaa aaaaacataa aaaaacataa aaaaattgtt gaaaaaaata
    39661 tagaaatttt gtataattaa agatttgaaa agtgcggtat acaaagccaa ttcttttgta
    39721 tataatatat ataaattcag aaaaaggaag ggatatgtat ggccaggcgg ttatttttag
    39781 aaaggattgg acttatattt ttaggttcag caatattagc atttggagtt tataattttt
    39841 attatttaaa caacataaca gaaggtggag tgttaggtat tttactactg ttaaaaaatt
    39901 tattcaatat acaaccagca atagcaaatg ttgtaataga tagtttacta cttttagtag
    39961 gatataaatt tttcggtaaa aaattcctta tatattcaat agtagcttct ataacttttt
    40021 cagtgttata tgacttattt gaagctatag gacctctagt accacaatca caaaatatgc
    40081 ttttatcaac tattttggca ggtgtaactg ttggaactgg tgtaggtata gtagtaaaag
    40141 caggttgtgc atcaggagga gacgatgctt tagcattagt aatttctaag actacatctt
    40201 tgaacatagg gcaagtttat ttagcaacag atgttattgt attgttgtta tcattatttt
    40261 atctatcagc gtttgatatc ttttattctt tgattgctgt tacaattagt ggcaaggtaa
    40321 ttgattttat ttattatcat ggtaaaagtt tggatatgga tataagtaat gatatagttc
    40381 cagaatgtta atataaatta aataacttta gattttatta ggggtggcag atttgccacc
    40441 ttttttttgt ctaaatcttt atagaaagta taatcccatc taggtttttg caaatctcaa
    40501 atatagaatt atgttggaat gactgggaat acgattgcta gatgtgcatt ttaaaaaccc
    40561 aattttatat ttatgaaata ctaatcatta atggatgttt cttaatccta tctttatacc
    40621 taaaatgata agataaatga agtagcaata caaagctaaa atttgtaaaa gagattaaat
    40681 taaatggagg agctaaaatg ttggatatag ttatgatagc attgttattc ataggatttg
    40741 tttccatgaa gttacttgct gattggtgtg ggaaacaaac tgaaaaaaaa taatgtaatg
    40801 tatggaggga ttacctatgt ggtttctggc agttgtaatt ataatgttaa taatatattt
    40861 ggtatatgcg cttattaatc ctgaaaaatt ttaaacagta taaggctaat aagtaagttg
    40921 aattgggggg aacaattttt atggtgcaga tagtaatagt gttagcgatt tttatgattt
    40981 tagtaatacc tatgggcaag tatttatatc atatagctac taatcaaaaa acatttggag
    41041 atagactatt tgataaagtg gacaatttta tatataaggt atgtagtata gataagaaaa
    41101 aagaaatgaa ctggaaacag tatgcattag ctttattatt tacaaatgca gttatggtat
    41161 ttataggata tatcatactt aggactcaaa gtatgcatat ttttaatcca agtggaatta
    41221 aaagtatgga acaaggacta tcatttaata caataataag ttttatgaca aatacaaatc
    41281 ttcaacatta ttcaggtgaa tcaggacttt catactttag tcagatgaca gtgataatat
    41341 atatgatgtt tacatcagct gcaacaggat atgcggctgc tatggcattt gtaagaggtc
    41401 tagtcggtaa aaagaaaaca ttgggaaatt tttatgtaga tttaattagg ataacaacaa
    41461 gagtactttt accaggagct ttaattattg ggcttatatt agttacacaa ggagtaccac
    41521 aaacttttgc tgggacagaa acagtgacta ctatagaagg aaagcttcaa gatatagcta
    41581 gaggtcctgt ggcagctctt gaatcaataa aacatttagg aacaaatggt ggtggattct
    41641 ttggttcaaa ttcatcacac ccatttgaaa atccaacaat aatatcaaat atagtagaaa
    41701 tattatctat gatgatatta ccaggagctt gtgttgttgc attcggtcac atgataaaga
    41761 ataaaaaaca aggttgggtt gtgtttggcg caatgtcaat catattttta ataggtctag
    41821 tagtgtgctt taaagcagaa agtgcaggaa atcctatttt atcacaatta gggcttaatc
    41881 aaagtatggg aagtatggaa ggtaaggaag ttagatttgg tatagcacaa tcatcattat
    41941 tcacaacagt gactacttcc tttacgacag gtacagttaa taatatgcat gatacactga
    42001 ctcctttagg aggattagta ccacttttaa atatgatgct aaatgtagta tttggtggta
    42061 aaggcgtagg tctgatgaat atgttgatgt acgctataat agcagtattc ttatgtggat
    42121 taatggtagg aagaacacca gagtttttaa ctaagaaaat cgaaggaaaa gaaatgaagc
    42181 ttattgcttt acttattata ttacatccac ttttaatact aatgttctct ggtctttcag
    42241 tagcaatacc agcaggactt gaaggaattt caaatcctgg attccatgga ttatcacagg
    42301 tattgtatga gtttgcatct tcagcagcca ataatggttc aggatttgaa ggtctaggag
    42361 ataacactat gttttggaat ataacaacag gtatagttat gttctttgga agatatgtgt
    42421 ctattatagt actactagca atatcaagtt tgttagcatc taaaaaagct gtaaatgaaa
    42481 gtataggaac acttagaacg gataatttta catttacaat agttttagta cttgttgttt
    42541 taattgttgg tgctttaaca tttttcccag ccttagcgct tggacctatt tcggagcatc
    42601 tagtgttgtg gcactaaaaa attgggaggg agtaaagttt tatggaaact aaaaaaacaa
    42661 agtttattac aaaagatatt ttcaaaactt ccattataga agcttttaaa aaattaaatc
    42721 ctaaatatat gatgaaaaat ccagttatgt ttgttgtaga aattggattt atagtaacat
    42781 tgatacttac aatagtacct agtttgtttg gtgatgaagg caataactta agagcttata
    42841 atgggattgt aacaataatc ttatttataa cagtcctttt tgcaaatttt gctgaagctg
    42901 tagctgaagg aagaggtaaa gctcaagcag atacacttaa gaaaactaga aaagatacag
    42961 tagcaagggt tatagatacg ttaggaaatg aaaaaatgat aagtgcaagt gagctaaaac
    43021 ttggtgatat tgttttagta aatgctggag aagttatccc aaatgatgga gaagttatag
    43081 agggtatagc ttctgtggat gagtcagcaa taactggaga atctgcacct gtcatgagag
    43141 aatcaggtgg agatttcgca tctgttacag gtggtactac agttgtaagt gactggttaa
    43201 agataagaat aacaacaaaa ccaggagaat cgttcttaga taaaatgata gctcttgtag
    43261 aaggtgcttc aagacaaaaa acgccaaatg agattgcttt aaatactttg ttagtagggt
    43321 taacacttat atttatggtt gttttaatat ctctttatcc aatggcaaac tatgcaggtg
    43381 taaagattcc aatttctact atgatagctc ttttggtatg tttaatacca actactatag
    43441 gtggattgtt gtcagctatt ggtattgcag gtatggatag agtaactaga tttaatgtaa
    43501 ttgcaatgtc aggaaaagca gtagaagctt gtggagacgt tgatactatg attttggata
    43561 agacaggtac aattacttat gggaatagac ttgcatcaga atttatagca gttggaaatg
    43621 cagattcaaa agatttaata aattattctg ttatgtgttc attaaaagat gatacaccag
    43681 aaggaaaatc tatagttgat ttaggtatga aattaggttc aacaggtaag acaaaagagg
    43741 cagaggaagc tgagtttgta gaattttctg cacaaactag aatgagtggt attaacttag
    43801 ctgatggtag agctataaga aaaggtgctt atgatagcat aataaaaaga gtaaaagaat
    43861 ctggtggtac agtgccagaa gatttagaag aaaatgtaaa tcgagtagct aaactaggag
    43921 ggacaccttt agtagtgtgt gtagataatg agatatatgg aattatctac ttaaaagata
    43981 cagtaaaatc agggcttgta gaaagatttg ctagacttcg agagatagga ataaagacaa
    44041 taatgtgtac aggggataat cccttgacag cagctacaat tgcaagagaa gctggagtag
    44101 atggatttat tgcagaatgt aaaccagaag ataaaataga tgcaataaaa aaagaacaat
    44161 tagagggtaa aatagttgca atgacaggtg atggaacaaa tgatgctcct gcacttgctc
    44221 aggcagatgt aggtcttgca atgaatagtg gtacaacatc tgcaaaagaa gcagcaaata
    44281 tggtagattt agattcagac ccaactaaga tattggaagt tgttgaaata ggaaaacagc
    44341 ttttaataac tcgtggggct ttgacaacat ttagtatagc aaatgatgtt gcaaaatatt
    44401 ttgcgattat accagctata tttactcttg caatacctca aatggagata ttaaatgtaa
    44461 tgaaactttc tacatcattt agtgcgatat tatcagcttt aatatttaat gctataatta
    44521 tcccatgttt aatacctctt gcaatgaagg gagtaaaata tagacctatg aaatcacaag
    44581 ctatgttaat gagaaatatg cttatatttg gtcttggtgg agttatagta ccatttttag
    44641 gaataaaatt aattgattta ttgattacac ctttagtaat ggttcttaat ttaggttaat
    44701 aaatggagga tataaaaatg aaaacattaa agccagcatt acttgtaagt attgtattgt
    44761 tagttgtttg tggacttgta tatccacttg ttttgacagg tgtaagccaa gttgcattta
    44821 aagataaagc caatggaagt atgatagaag taaatggtgt aaaagtaggt tcagaactta
    44881 ttggtcaaag ctttacagat gtgagattct ttaagggaag agtatcatca gtaaactata
    44941 atacttatac aaaagaagat ttagtaccag ataaagatgg aaacacatct tatggaggtg
    45001 tatcatcagg gtcttttaat tatggagcta caaatccaga acttcatgat agagtacaaa
    45061 aagatataga acagttttta aaggataatc caactgtcaa aagagaggat ataccaacag
    45121 atttacttac agcatcaggt tcaggacttg accctaatat aagtccagcc tctgcaaaaa
    45181 ttcagatacc agctatagca aaagcgtctg gaatatcaga aagtaaatta caaaaaattg
    45241 tagatgataa tacttccaaa aaactttttg gagttttagg tgaagataga gttaatgtat
    45301 taaaggttaa tgtagaggtc gcaaaaatat taggtcttat atagtatttt gtggaatttt
    45361 agcaacaaaa tattacgcaa gatttaattt aaaggttaaa ttttaaagag tgaaaatcga
    45421 taatgatttt cactttttta tttgaatatt actaatattt ggaataaagt gattgtaagt
    45481 ataaaatcca aaaaaaggtt gcattatcaa aatgataata ctataatata tttatcgtta
    45541 tcaatttgat aatagatttg gacatatagg aggaaatagt atgttatata ataacgcatt
    45601 agagttgata ggaaacacac ctgtagtaag gctgaataat ttaggctgta ctaatgggtg
    45661 ttctaacata tatgtaaaat tagaaaaagt aaatccagca ggaagtataa aagatagagc
    45721 tgtttatgaa atgttagagg gcttagagca aagaggagaa ttaaaaaagg gagatgtact
    45781 agttgaagca actagtggaa atactggtat tgcattatct atgataggaa aattaaaagg
    45841 atatgaagta gtaatcgtaa tgcccgaaac aatgagcgtt gagagaagag atttaatgaa
    45901 ggcttatgga gcaaatttgg tgcttacaga tggtaaagct ggaatggcag gttccataga
    45961 aaaagcaaat gaacttttaa aagaaaatcc aaactataaa agcctaaaac aatttgaaaa
    46021 taaagataat ccaaatgcac attataaaac aacagcagtt gaaattatga atgatgtcaa
    46081 agatttagat atattcgtat gtggagttgg aacaggtggt actttaatag gaactgcaag
    46141 atatttaaaa gaacaaaatc caaatataag agtaattgca cttgaaccaa aaaaatctcc
    46201 agctatatca gaaaacaaga ctggacctca taaaatacaa ggaataggtg ctaactttgt
    46261 tcctgaaaat tatgattcta gtgtagttga tgaagtaata ttagtagatg atgaagatgc
    46321 atttgataca gttaaattac ttgcagcaaa agagggaatt ttagtaggga tatcttctgg
    46381 agcaaatata tttggtgcta tagagatggc aaaaaagtat ccagataaaa agatagttac
    46441 agtagctcca gatggagttg ataagtatat gtctatggga atattttaat aattttattt
    46501 gagggagtga atactttgtt taagaaaata aataaagata ttgaatatat aatgaagaat
    46561 gatccagcag ctaggtctaa gatagaagtt tttttacttt atccatcagt tcatgcaatg
    46621 ataatgcata ggatggctca tgctctttat aagaagaaga agctttttac agcaagatta
    46681 atatcccaga tatccagatt tatgactggc atagaaatac atcctggtgc taaaatgggg
    46741 gaaggtatat tgatagacca tggaatgggt gttgtaattg gagagacagc ggaagtaggg
    46801 aatagggtga caatatatca aggagctact ttaggagcta caggaaaaga tactggaaag
    46861 agacatccaa ctgttggtga tgatgtatta attggtgctg gaacaaaaat actaggacct
    46921 ttaaatatag gttcaaattc aaaaattggt gcaaattctg ttgtcgttaa ggatgtacct
    46981 aatggtgcaa cagttgttgg tataccagca aaaatagtaa aaataagaaa cctagaacct
    47041 gtaaaaaaga ataaaaaaga agtgtcctat gagtatgatg aattagataa tgtatattat
    47101 atataggatt tatataacta taaaataaac tttataaaaa ttattttaaa aggtattttt
    47161 ttcaaatttg aggacataat aaaaaagaac tgaatgttac agttttggtt catcaatctt
    47221 tgataaacaa gattttaaat ttgtagcgtc taggaggatt ttattatgaa agcaaatgtt
    47281 aatcaagata cttgtatagg ttgtggatta tgtccatcaa tatgtccaga agtatttgat
    47341 atgaaggatg atgggaaatc acatgttata gttgatgaag taccaagtga tgctgaagaa
    47401 tcagcagcag aagctagaga aagctgtcca gttgatgcaa tagatgtgca ttaataaaat
    47461 aaatgcctat aaaaattgaa ttttcaattt ttataggcat ttgtctttta ttcagttagt
    47521 atttcaacac ctgtttctgt aactaaaacc atacattccc attgagcaga taatttacca
    47581 tcttttgtat aaatagtcca attattttct tcatcaacaa aaatttcgtg agttccttca
    47641 tttaccatag gttctattgt gaatatcatc ccaggaacca atagcatctt agtccctttt
    47701 ttagatacat aacttacaaa agggtcttca tgaaagctta atccaactcc atgaccacct
    47761 atttctctaa caacagagta tccatttttc tttgcataag agttgatagc atcacctata
    47821 tctcctaaaa atccccatgg tttggcaact tcaagacctt tttggacaca ttcctttgta
    47881 acttgaacta atttttttgc attttcatta acattaccta tcataaacat tcttgaagca
    47941 tctgaatagt atccattaaa aatagtagaa acatctacat ttataatatc tccatctttt
    48001 aagataatgt ccttacttgg aataccatga caaacttcat tatttactga tgtacataca
    48061 cttttaggaa atccattata gtctagtgga gcagggatag ctccattttg tagtgtaaag
    48121 tcatgaacca acttatttat atcttctgta gacattccaa tttcgatttt atctgaaata
    48181 taatccaaaa tttttgtgtt tattacagca ctttttttta tttcctcaat ttgactttca
    48241 ttttttataa tatcatgagt tggtacaata tgacctaact tagcaaatga atctatttta
    48301 ttatccatat ccatatgaca ttttttatat tttttagtac ttccacacca acagctatca
    48361 tttctattta acttcataac ttttcatcct tcttttatta tttatttaaa ttatgggtta
    48421 cataaactat tttaaaacat aaccattata aatattataa cataaatact aagaaatcct
    48481 ttatagatag tgatttattc aaagtataaa ttattatatg gttattgttg atgaaaagag
    48541 gatagttaaa tgtattattg gatattagcg cacttaatag tttaattcaa gtttttcaag
    48601 aaaatcatat gttagaggac aattttcctt taaagtattt tttgaatctt catcaagata
    48661 ataatgcaca aatgattctg caaaaaattc ttctataaac ttgcttatat atgcatgtgc
    48721 aacttgctta gggtcataaa gcttatatct ttcctgagca aatacttcca taaattcttc
    48781 agaatttgat tttttattta aaacattttt atctatagca tgtgcagttt catgtaattc
    48841 taaatttttt gatgaatgta catttgcata gctagggtta ctataaccta ttttagccac
    48901 tatagaatta tttctatata taccaggtac atctttccaa gtcttgcctg atcttaccca
    48961 acatggaggt aattgatatt ttaaatcact aaattctggt tcatcagtta gatttgaatt
    49021 tattaatttt atttttatat ccttttcttt aagtgtctgt aaatatttaa tatttatttt
    49081 tgcaagtctg tttttcatat tttcaacttc atctttatca taattcaagt catcaggaag
    49141 tataagcatt tgttcaatta tatctatact actgtctttt atattttcag attcttcatt
    49201 aaatttatat tgaagagtat tattttcata gatagttata tttgtattat tttctataga
    49261 ataagagtga atatcctcta aatctaaaat attcatttgt tgtaaaatat gttttgtttc
    49321 gccactccat actactaaag aaagtgaatt aaaattaatt agtggatttt cttttgcttt
    49381 tatatcattg cttatatcta catcaaatac atattcaatt gagtcttcta tgttaagact
    49441 atctgtattc tttttaatat ataaatacgc ttgttttaag tctatataaa tctcatctaa
    49501 agattcgtca taaaattctg ataaattatt agtaaagagt tcttcactta aaagttcatt
    49561 tttatcagtt atactttcaa ttaaaatatt aatttctttt atcatatcgt aaatacttat
    49621 ttttctattt gctttttcac tagatacatt gcatggcgca agagcaatgc aataaaaatc
    49681 tttttctttt ttataaaagg ctttaagtga ttttgataaa agtgtaaagt ctacacctat
    49741 ctgtaagttg accttaagtg agtcattttg taataattca gtattatttc ttttagctgt
    49801 atttaaattg ttttccataa tcattgcccc ctttttatta gggtatcata atttttttat
    49861 atgtgtcaat ttgttttttt gaaaattata atttttttga aaattttatt ttttataatt
    49921 tgtgaatttt ttattataga aataaatatg ttattattat
//
LOCUS       DAGSRM010000022        49017 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.022, whole genome shotgun sequence.
ACCESSION   DAGSRM010000022 DAGSRM010000000
VERSION     DAGSRM010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 49017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49017
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.022"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            32..1144
                     /locus_tag="MJE33_001364"
     CDS             32..1144
                     /locus_tag="MJE33_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HBY9647305.1"
                     /translation="MNSRIKSIDIIRGLSIALMIVCNNPGTWMRMYPQLRHAVWHGVT
                     LADFAFPFFVISLGVTIPISINSKLKNNKSTLSIILSIFKRSILLILFGFFLNYLGNP
                     DLDTVRILGVLQRMGLVYFVTSLAYLLLKKLNVGSTATIITFLYIATFIIVGYYILAK
                     PYGFELEGSLAQLVDLHFFKGHLYKPEFEPDGFLTSIVAISSGMLGCTMGCVLLKENL
                     GEYKKFFKILVMSIILLIGAFIFNQYFPFNKRLWSSSFVLLMAGSYGILLSIFYFICD
                     IKNKSKIFTPIIALGSSPIFTYMCLEILSHVFWNVPKLTNKVDYPTTLVEWTTYELIT
                     PWAGTTWDSLIFSLLYVLFWVIVMSIMYKKKIFIKI"
     gene            1398..3422
                     /locus_tag="MJE33_001365"
     CDS             1398..3422
                     /locus_tag="MJE33_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBY9647306.1"
                     /translation="MKKTTKLLATGMLSVAMVAPNVALAAENTTANTESNSDININLQ
                     RKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNKD
                     PEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNEY
                     MRKTSAPVKKLAGATRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYDA
                     PILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNVTRIGGVD
                     RHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQG
                     TYNWLSSQGLEEAYYIGGSQSLSSKIIDQISKIAKNGTSKNRVSGADRHETNANVIKT
                     FYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHNTNLSEKTAET
                     VYQIGDGMKDSVINSIASSLSKHNAPTEPDNSGSAAGKTVVIDPGHGGSDSGATSGLN
                     GGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSIH
                     YNASNGAGNGVEIYYKVKDKNGGTTKTAASNILKRILEKFNMKNRGIKTRTLDNGKDY
                     LYVLRNNNYPAILVECAFIDNKSDMDKLNTAEKVKTMGTQIGIGIEDTVK"
     gene            3642..4328
                     /locus_tag="MJE33_001366"
     CDS             3642..4328
                     /locus_tag="MJE33_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBY9647307.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            4423..5478
                     /locus_tag="MJE33_001367"
     CDS             4423..5478
                     /locus_tag="MJE33_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HBY9647308.1"
                     /translation="MIKKISTILSLVLLISISSTIGVFADANPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEESIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAVSKDAERNIEKMGMKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEGFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTARLDFGSRSDVNEYISLGVV
                     SAKENMPILICSDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFKSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            5593..7149
                     /gene="murJ"
                     /locus_tag="MJE33_001368"
     CDS             5593..7149
                     /gene="murJ"
                     /locus_tag="MJE33_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBY9647309.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTVFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGDGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDNKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVATLLIYNNLYAFMGSGTIKEIISVGAGVLGGASVYTVLIVLFKVEEMDLAFEFL
                     RKGKQKLLRR"
     gene            7195..8901
                     /locus_tag="MJE33_001369"
     CDS             7195..8901
                     /locus_tag="MJE33_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBY9647310.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKNLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIMKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDMANDIIAEVNKIDDYSTIKSIDFEEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDEYGKKLKIIYTPIHGTGNKPVRKVLNECGFENVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKNNGTDLIIGTDPDCDRVGIVVKDSSGEYVVLNGNQVGSL
                     LVRYILESLVEENKLPKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEESNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALID
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKDGVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVEFKLNNLISYILNVVD
                     SI"
     gene            8923..9996
                     /locus_tag="MJE33_001370"
     CDS             8923..9996
                     /locus_tag="MJE33_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HBY9647311.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDSNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKDEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEDISKYLGTKEEMEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            10008..10730
                     /locus_tag="MJE33_001371"
     CDS             10008..10730
                     /locus_tag="MJE33_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBY9647312.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDMQILKDITPLLENNR
                     LVCGYEDDREYINGAIIGVEKGHPFLKDLLEYYEKEVLTSSLFTIPKIMTHLMEKNYK
                     KIDPNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTFGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            10746..11903
                     /locus_tag="MJE33_001372"
     CDS             10746..11903
                     /locus_tag="MJE33_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HBY9647313.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNMDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTIYAAYIFLRYKDYNKWDRILLFGSAFFVYQTTYSRTGFFTILAGLIFVEI
                     IRWIDIKKIKGLPMLLKTLPVILTLFSVIIGTVFDKSTLLNRLLASRPKFWNVYLAEK
                     GNFLKPFGNSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKA
                     YLVALFIFLLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            11906..12745
                     /locus_tag="MJE33_001373"
     CDS             11906..12745
                     /locus_tag="MJE33_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBY9647314.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNKDYKIYSCKTMDSNKVDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGNDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYKFAKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            12760..13935
                     /locus_tag="MJE33_001374"
     CDS             12760..13935
                     /locus_tag="MJE33_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HBY9647315.1"
                     /translation="MDKVKFLINMLLAFFIYPFNKHKFNGRDIWLIGGHSGDIYNDNS
                     KFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIVFSHAP
                     SADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKSYNIVT
                     ASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWIKFSSG
                     KFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGKRIVFL
                     DKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYIDLKSEM
                     FGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVLSKIK"
     gene            13964..14713
                     /locus_tag="MJE33_001375"
     CDS             13964..14713
                     /locus_tag="MJE33_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBY9647316.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYRRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            14735..15643
                     /locus_tag="MJE33_001376"
     CDS             14735..15643
                     /locus_tag="MJE33_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBY9647317.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLEIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALRGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            15691..16443
                     /locus_tag="MJE33_001377"
     CDS             15691..16443
                     /locus_tag="MJE33_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBY9647318.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENVGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            16397..17764
                     /locus_tag="MJE33_001378"
     CDS             16397..17764
                     /locus_tag="MJE33_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HBY9647319.1"
                     /translation="MYFVSMFLMPLKKEYKFYILGGKKMKIAIAGTGYVGLVTGVCLA
                     EVGNENVVCVDVDEQKVKSMKLGIAPIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDV
                     DVILIAVGTPERSDGSANLDYIKAVAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYIN
                     RNLKNDVKVELASNPEFLSQGTAVKDTLHASRIVIGVESEWAREILEEIYKPFNQPII
                     VTNRNSAEMIKYASNNFLALKISFMNDIANLCEIAGANIEDVALGMSYDDRIGSKFLK
                     AGIGYGGSCFPKDTKALHWLSEHNGYELKTVKAAIEVNESQKLKLVRKASKLVESFQD
                     MKVAVLGLTFKPDTDDIREAPSIPNIKYLLERGARVTVYDPIGVNNTKKIFGDSILYA
                     DSVDDAIEDAEICFIMTEWKDVLNYDIHKYKEKMKNPLVLDGRNCYKIKDMEELGIEY
                     YSIGR"
     gene            17770..18861
                     /locus_tag="MJE33_001379"
     CDS             17770..18861
                     /locus_tag="MJE33_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBY9647320.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSIKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLAKELGIENDVQFLGRISPENVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWENETGLIVEPKQPEEIKNAIIKLMENDELRLNMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            18945..20858
                     /locus_tag="MJE33_001380"
     CDS             18945..20858
                     /locus_tag="MJE33_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBY9647321.1"
                     /translation="MRESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNAFTTNDIIISVCIYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFVATLAYLI
                     PIYIINKLLGFDYPIMFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKVSNILII
                     GAGDAGEMVIQELKRNPELKKVAVAIIDDDKNKIGRIIHNVKIVGTTSDIKDVVEKYN
                     VDEIIFSIANIEKRRKKDIIDICKNTNCKIKTIPGIYEIIDGKVDIKQIREVEIEDLL
                     GREPIKTNLREISNYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIAGLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            21057..21764
                     /locus_tag="MJE33_001381"
     CDS             21057..21764
                     /locus_tag="MJE33_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBY9647322.1"
                     /translation="MMNIKNKKHILKKFIAMVLIAGVVTVEAGAITASAAEPTNSPMS
                     ATVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKSVDNVTGWVNGDY
                     LTIQGGNVDAKVQNVLNLAFKQQGKPYKWGATGPNSFDCSGFTSYVYKNGAGVNLPRV
                     SRSQATVGKKVSRAELKPGDLVFFGSGGSINHVGLYVGDSKFIHSPQTGDVVKVTSMA
                     PGTNYAKRLITATRVLQ"
     gene            22066..24177
                     /locus_tag="MJE33_001382"
     CDS             22066..24177
                     /locus_tag="MJE33_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HBY9647323.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANHPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVQNYDIDGVHFDDYFYPGVSFNDTATYQKYGKGQN
                     KDDWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVAWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSTSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWATNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNNSIGDKVESEIKDTLSNVSINRVGG
                     SDRYSTSLMIAKELVKTNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTTL
                     VYEAGDGINQNTLNTFLNLVK"
     gene            complement(24375..25340)
                     /locus_tag="MJE33_001383"
     CDS             complement(24375..25340)
                     /locus_tag="MJE33_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBY9647324.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLD
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTVEK
                     GEIRELNKFIPETYKWNKSDDKGSIQYIRNSGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
     gene            complement(25625..26581)
                     /locus_tag="MJE33_001384"
     CDS             complement(25625..26581)
                     /locus_tag="MJE33_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBY9647325.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVNIP
                     GRGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDK
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPNLVNTILPYVKTNMNPNEIISLGKELLSIGNLNLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
     gene            26949..27737
                     /locus_tag="MJE33_001385"
     CDS             26949..27737
                     /locus_tag="MJE33_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBY9647326.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEDIKDDMMFISENGTLVVYKGKEILSNPLNKELVNEIIETTRSIKGKKI
                     VMSGKKYAYIESKDEAFIQEVSTYYAKFKIVEDLTKVEGDILKIAVFDFKGAEHNNNI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEHLKQIARFRAKRNTENGVVDKIKEVIKIG"
     gene            complement(27860..28330)
                     /locus_tag="MJE33_001386"
     CDS             complement(27860..28330)
                     /locus_tag="MJE33_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HBY9647327.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVEEDIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKTLENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENATQIENKILNIIKK"
     gene            28469..29113
                     /gene="uppS"
                     /locus_tag="MJE33_001387"
     CDS             28469..29113
                     /gene="uppS"
                     /locus_tag="MJE33_001387"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HBY9647328.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDVTLGG"
     gene            29156..30061
                     /locus_tag="MJE33_001388"
     CDS             29156..30061
                     /locus_tag="MJE33_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBY9647329.1"
                     /translation="MKKKLLDGKITLIICKSVKIYTKKGEEMSKGNNNNNSRNKSKKT
                     SHLNRKKRKLNKKKLAVLICFTVLFLFIAFKATQGVVALVKSMDKSNKTNQQQNVNSE
                     QFDLGNEEENKKKKYTVFIDPGHGGNDKGTESKTSNRYEKDLNLQIAKKLANKLSKQK
                     DIQVVVSRTDDTYISLKDRAILANNSSADVLVSIHLNAEKNGNTATGIETWYRNKATD
                     GSKELAQTVQSTIVSYVKVRDRGIVENNFEVLRESNMPAILIECGFLTTPSEEQKIIN
                     EKYQDQLAEGIVQGVLSYLDSKGNK"
     gene            30376..31923
                     /locus_tag="MJE33_001389"
     CDS             30376..31923
                     /locus_tag="MJE33_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBY9647330.1"
                     /translation="MNKNSMTRIYSLNKIIFIIIGILGIVLLTRHIEVDIKVLILILL
                     TLFSINVFISYLKIKLYEDKINLGLINNSDKFINITKDENFLKHVQNYIISNEKENCV
                     MACFDLCRLKLINDIYGYELGDEVLNNILSNLKNYFGKEAIYGKLKSDVFVLIIKLEN
                     REQKIPYLVNLIKNKIVILSQIDSFKMDINIEASIGIYKFNNNEIDIKKAIDNADMAR
                     LKSKGLKHIEYVEFDNAMEEEIQSIMKIERDLFLAIKNKEFVIHYQPKVDSSTGNIIG
                     SEALIRWLHPSLGMVGPNKFIPIAEKNGLINNIGRWLIQEVFITINKWISEGINVLPV
                     SINLSRVELYKNDLVDFFKLMFNTYNIPKELIELEITETTALRDVKFISERLYEIKSL
                     GMNISLDDFGVGNSNFINLKGIPLDIIKIDRSLILDIVTNTKTKFMVKAIVNLSHDLN
                     VTVICEGVEDMHQVKVLSELGCNFIQGYVFFKPLDEMNYKKLLTDGSYINLKDTLLDK
                     CMTVEEE"
     gene            31926..32324
                     /locus_tag="MJE33_001390"
     CDS             31926..32324
                     /locus_tag="MJE33_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBY9647331.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDKPIDAKMILKSEDIPILTLYRL
                     LRKLCANNILTFNKEDGKTYMLNVSLDTISLRTIVEVIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            32364..34097
                     /locus_tag="MJE33_001391"
     CDS             32364..34097
                     /locus_tag="MJE33_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBY9647332.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWTVDNIH
                     RHILNYCIYGADIDEKAISILKDSLTNKKVVNDLDESDIKINLFCCDSLKKKWRYKFD
                     YIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGIGSVIT
                     PRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYID
                     VFKIKNEDICINKFETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETFYNKIQEKC
                     KYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINKYIVDKSEY
                     RLIYSNDIDNENTNKRILDEIIGLYKTKLENRRECKSGIRKWYELQWGREKLFFERKK
                     IMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEYLDKFSYEYLVGILNSSVYDKYFKI
                     TAKKMSKNIYDYYPNKVMKIRIFRDNNYEEIENLSKQIISILLNKSIDKGKVEKLQIK
                     MDNLIMDSLGI"
     gene            34329..35540
                     /locus_tag="MJE33_001392"
     CDS             34329..35540
                     /locus_tag="MJE33_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBY9647333.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIVQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDASLRTVMESVIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRNLASNFEVRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            35733..35993
                     /locus_tag="MJE33_001393"
     CDS             35733..35993
                     /locus_tag="MJE33_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HBY9647334.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
     gene            36347..38059
                     /gene="ptsP"
                     /locus_tag="MJE33_001394"
     CDS             36347..38059
                     /gene="ptsP"
                     /locus_tag="MJE33_001394"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBY9647335.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNVEAEIAKLED
                     AVAVSKEELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTESVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHILGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDEETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEIQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLSMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASVHGRLRIMFPMI
                     SSLEELLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADDVFNMGTAEEIKSYIEK
                     TFNI"
     gene            38382..39932
                     /locus_tag="MJE33_001395"
     CDS             38382..39932
                     /locus_tag="MJE33_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBY9647336.1"
                     /translation="MRYKKRNIDKSSELSFQSLAKYFITFYIVIILIILSMFCINTIW
                     VMKKDNQDLLSTTSNRIEDRVGETIKLLNSLSKDNYIIDNSVNGIDKSKELSMYSDEF
                     GYMMICFVDKNIIVWDEEGGNISLAGRDYMQKLFATKKYQVTDSFAAGADGKTLNYTI
                     ASPIIKNGNVDGCVFAAIYFDEIKDILKQGKGQQQQNFILFGSENQIMNLTSDETAYG
                     EKYMKLSQKTKYLGTNAKKVEKDMFNNKSGDYWAFENGKLYFMSYKPIKNTNWSLFCK
                     VNFLDIFALNLIPMGAILSVITLVFIILICMEKSFMKKRMEVIDMLLGSVQELEKRIY
                     QTERPSEVDFKEIIQLTSKGLTDGLTGVVTRTVFFDQIYNRVEMEENDKLSVISFVDL
                     DDLKCINDLYGHEIGDMVLKNVGYVLREYEIKYNGLIGRYGGDEFVMLLSGFTSREEV
                     EDLLDEIAIRLHTEVLTDDGIIQTHCSVGAVVCSVKDNKDIDNLLKQADEALYYVKRN
                     GKGNYHLV"
     gene            39958..42660
                     /locus_tag="MJE33_001396"
     CDS             39958..42660
                     /locus_tag="MJE33_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBY9647337.1"
                     /translation="MKNVSKKFFVCTIIVLITFLCFSKFAIYVFDKQEKKETKVVENM
                     LNVYKNNIILNLTEKFNYSKDLTSVLVSNNYSIDKLKPIADEILKNNSCIYISVVEKY
                     NVAAFFSNSVKKDIINTPIQELNYSYSLANSIKGLVVEGPSKDQFSNGDVFLITNPIY
                     KNGEYWGEITVALDAKKVVDSFNLNKLKESGFEYELWRVHLDNGSKSTIEISTKDFNF
                     SKSHEIKFKLPTVWTLSIVYKNGWNNKNIDFIVFVILLIISILIGVMTFIYIEKKSIS
                     KELNQQSLKNLETGILNREGFLIKLQEHLNRKNNEIVLLYICITNYSKFAPVMSFEKR
                     QDYLTHIVKNLDNYIKSSHFIARISEEGFVLALEENCDKNELLNIEKGISLELLRKIK
                     VENKQVFLTIGYGSSRCVDDRKDAESLLLSAIEDYERRFPAMSPIHDFTKKCEDMLDE
                     KKQIVFGEYPDLEINKLSKVIYKYYKKVQRIMYKDYNIDIGNRRGYMRDVEVLIDYNS
                     ERMLYLFLIDICKFGKFNELFSIEIGDVILKEVSNKLKTIFSNYLYRINGDVFLGISI
                     DKESYLDTLHKVNEVFNSSLSVEDVLFDIKCNIGICQYPYHGENPYDLLEKVQIALEY
                     AKKDVKSNVKVYDKELTEEIYHENNIIKDLKKGLEEQTLEVWYQPIFNVSKNCYIKAE
                     ALIRLRNKEGGYYSAYEVITYAEKHGLIKDVGLYVLKKACDVTTKIKDKNIDFEQMQL
                     NLSVQQIVHKQCANDILSCIENCGLDKKYICVEVTETVLMETFEIVNVTLDKLKKEGI
                     SIALDEFGSGFSSINYLANLPIENLKIDKELVEQITYSKKQFELLKTIVQIAKINGMD
                     IVAEGVETKETLDLVVTSGVNLIQGHYFSEPLSEDKFIELMIEE"
     gene            complement(43166..43831)
                     /locus_tag="MJE33_001397"
     CDS             complement(43166..43831)
                     /locus_tag="MJE33_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647338.1"
                     /translation="MKGNIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCK
                     ESIESFCLGKINNFVEILNEGFVENHHYVHLDKMISVPKKKVNLVYQQDTHGYLLRDD
                     NDNLIPAKITSEQSKSISSKMELFSAGEEKCLINILLKADPSYILDVDSIKDKNILNL
                     GYESIDRYKEYNFDDDKILIFFINKKRYSVIMKKTNNSDNDLVSRNNAIKELFTNKAG
                     NLN"
     gene            44175..45605
                     /locus_tag="MJE33_001398"
     CDS             44175..45605
                     /locus_tag="MJE33_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBY9647339.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEEDLKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEICLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKSLKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEEQTEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     EAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTENICIEAVRGSFEELEIKE
                     ILSYVKIPTERIFVEAVKQNGKILKYVENQTELICLEAVRENYNALAYVKEQTEKICL
                     EAVNQSYEALKYVKEQTEEVCLKAVKQDYRMLKYVNNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQS"
     gene            45973..46551
                     /locus_tag="MJE33_001399"
     CDS             45973..46551
                     /locus_tag="MJE33_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBY9647340.1"
                     /translation="MFKRYAEKMTSVLICNNMIDNNESKVYSYGFEILIAFIVNITTM
                     LFIGFLFGKFTYVLFFLMCYCPIRQFSGGYHADNYFRCLLTFIFIILSTILIIENINI
                     DLFKNIIMIIASVSWVGICVLCPIEHRSNPISDREKLVYKKTAIFISTVVLLITILSL
                     SLSIFVDYFTYSAFAMFWIFVMLVLGKLKAKV"
     gene            46581..46727
                     /locus_tag="MJE33_001400"
     CDS             46581..46727
                     /locus_tag="MJE33_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBY9647341.1"
                     /translation="MKKFIVRFMKFASSLALSTAILSANSTCPWIIHQPKVPKEISNL
                     KKTN"
     gene            47032..48411
                     /gene="scfB"
                     /locus_tag="MJE33_001401"
     CDS             47032..48411
                     /gene="scfB"
                     /locus_tag="MJE33_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HBY9647342.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVDYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSVTPNGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNNDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            48440..48937
                     /locus_tag="MJE33_001402"
     CDS             48440..48937
                     /locus_tag="MJE33_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBY9647343.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKKYIDESYEWYLEAA
                     QNQKY"
BASE COUNT        19563 a         5000 c         8592 g        15862 t
ORIGIN      
        1 aatttcaaat aatgctgtag attctaaact tatgaatagt aggattaaat caatagatat
       61 aataagaggg ctatcaatag ccctcatgat agtctgcaat aatcctggga cttggatgag
      121 aatgtatcct caattaagac atgctgtatg gcatggtgta acacttgcag attttgcatt
      181 tccttttttt gttatatctt taggtgttac aattccaata tcaataaata gtaaactaaa
      241 aaataataaa tccacactga gtataatact tagtatattc aagagaagta tcttattgat
      301 attatttgga tttttcttaa attacctagg aaatcctgat ttagatactg taagaatact
      361 tggagtactt caacgtatgg gattagtcta ttttgtgact agtttagcat atttactgct
      421 aaaaaaatta aatgttggaa gtactgctac aataattact tttctatata tagctacatt
      481 tataatagtt gggtactaca tcttagcaaa accttatgga tttgagctag aaggttctct
      541 tgcacagtta gtggatttac atttcttcaa aggacatttg tacaagccgg aatttgaacc
      601 agatggtttt ttaactagta tagtagcaat ttcttcaggt atgttgggat gcactatggg
      661 atgtgtactt ttaaaagaaa acctaggaga atataaaaaa ttctttaaaa tactggttat
      721 gagcataatc ctgctaatag gtgcttttat ctttaatcag tattttccat ttaataaaag
      781 attatggtct agctcatttg tacttttaat ggctggcagt tatggtatat tactgtcaat
      841 attttatttt atatgcgaca tcaaaaataa aagtaaaata tttaccccaa taatagcact
      901 tggaagtagc cccattttta catatatgtg ccttgagata ttgagtcatg ttttttggaa
      961 tgttccaaaa cttacgaaca aagtcgatta tccaactacc ttggtagagt ggactacata
     1021 tgaacttata acaccttggg caggtacaac ttgggattct cttatatttt ctttattgta
     1081 tgtcctattc tgggttatag taatgtctat aatgtacaag aaaaaaatat ttattaaaat
     1141 ttagtgcaat atttttagat gataaagtct ttgatttgtt caaatataca gaaactatca
     1201 aatataatca aaatttctcc tcgataaaat ttaaaaacca tgccataaaa gattacaaaa
     1261 agtttacaat ttgtaatttt ttgtgctata atgaaaaaag atttacataa tattgaattt
     1321 atgtaatata aaacaagctt aagatgtatt aaaaaagatt taaagattta aagaaccaaa
     1381 aaattagggg ggatatgatg aaaaaaacaa caaaattatt ggcaacagga atgttgtctg
     1441 tagcaatggt ggcaccaaat gtagctttag cggctgaaaa tactactgca aatacagaat
     1501 ctaattcaga tattaatata aatttacaaa gaaaaagtgt agtgttagga agtaaatcta
     1561 atgcaagtgt aaaatttaaa gaaaaattaa atgctgattc aataacatta aattttatgt
     1621 gttatgatat gccacttgaa gctactttaa attacaatga aaagacagat tcatatgaag
     1681 gtgtaataaa ctataacaaa gacccagaat acttaaatgt atgggaactt caaagcataa
     1741 aaataaatgg taaagatgaa cagaaagttt taaacaaaga agacttagag tctatgggat
     1801 taaatcttaa ggattatgat gttacacaag agtttataat aagtgatgca aattcaacaa
     1861 aagcagtaaa tgaatatatg agaaaaactt ctgcaccagt aaaaaaactt gctggagcta
     1921 ctagatttga aactgctgtt gagataagta aacaaggttg gaaagatggg tctagtaagg
     1981 ttgtaatagt taatggagaa ttagcagcag atggaattac agctactcca cttgcatcta
     2041 catatgacgc accaatatta cttgcaaata aagatgatat accagaaagt acaaaagcag
     2101 agttaaaacg tctaaatcca agtgatgtaa taattatagg tgatgatggt tcagtttcac
     2161 aaaaggcagt gtctcaaatt aaatctgctg taaatgtaaa tgtgactcgt attggtggag
     2221 ttgatagaca tgaaacatct ttacttatag caaaagaaat agataagtat catgatgtta
     2281 ataaaatata catagcaaat ggatatgctg gtgaatatga tgctcttaat atttcatcaa
     2341 aagctggtga agaccaacaa cctataatat tagctaataa agattctgta ccacaaggta
     2401 catataattg gttatcttca caaggtttag aagaagctta ttacataggt ggtagtcaaa
     2461 gtttatcatc aaaaattata gaccaaatat ctaaaatagc aaaaaatggt acttcaaaaa
     2521 atagagtttc tggagcagat agacatgaaa caaatgcaaa tgtaattaag actttctatc
     2581 cagataaaga gttgagtgca atgttagttg caaaatctga cataatagtt gattcaataa
     2641 cagcaggacc tttagcagct aaattaaaag ctccaatact tataactcca aaaacttatg
     2701 tttctgcata ccataataca aatttatcag aaaaaacagc agaaacagta tatcaaattg
     2761 gtgatggaat gaaagatagt gttataaatt ctatagcatc tagtttatca aaacacaatg
     2821 caccaacaga accagataat tcaggttcag cagcaggaaa aactgtagtg atagacccag
     2881 gtcatggggg ttctgattca ggggcaacaa gtggattaaa tggtggagca caagaaaaga
     2941 aatatactct taatacagca ttagcaacta ctgaatattt acgttcaaaa ggtataaatg
     3001 ttgttatgac tagagataca gataaaacaa tggctttagg agaaagaaca gcattatcaa
     3061 atactataaa gccagattta tttacaagta tacattacaa tgcgtctaat ggagctggta
     3121 atggtgtgga aatttattac aaagtaaaag ataaaaatgg tggaacaact aagactgcag
     3181 catcaaatat cttaaaaaga atactagaaa aatttaacat gaaaaataga ggaatcaaga
     3241 caagaacact tgataatgga aaagattacc tatatgtgtt aagaaataac aattatccag
     3301 caatacttgt tgaatgtgca tttattgaca ataagagcga tatggataag ttaaatactg
     3361 cagaaaaagt aaaaacaatg ggaactcaaa ttggaatagg aatagaagat acagtaaaat
     3421 aaaatagaaa atattaaagc aaaacatatc atctcctgtt ataatgtaaa atataacaag
     3481 agatgatata tttttgtaaa gaatcaaatt gtttacaatg atgtttcttg tattgataat
     3541 ctgctcatta ttgagtataa tatagaaata agtcaaaaat aagatagaaa taagttgaaa
     3601 ttaagaatta aaaaaatttt tattccaata tggaggtaac tatgtctgga tatactaatg
     3661 atgagtgtga aatacctaaa attaaaagct atccaggggc agataaagaa attgcaagtg
     3721 agattgacta tagcatagta aaaggaacag ttttattcga cttgtaccaa agaattatgg
     3781 atttagtttt atctataata ggacttgtga taggtttacc tttaatagca atatttggaa
     3841 ttcttattaa aatagaggac aaaggaccaa taacttataa gcaagaaaga ctaggaaaat
     3901 gcgggagaag attctatata tataaattga gatctatgag aacagacgca gaaaaatttg
     3961 gcgcacaatg ggctgaaaaa gatgatccta gaataactaa ggttggaaag tttattagga
     4021 aaactagaat tgatgagatt ccacagctgt ttaatatatt aaagggggac atgggcttaa
     4081 taggtcctag accagagaga ccgaacttta ctgttcaatt taatgaggaa atacctggat
     4141 ttatcaacag gctcgctata aaacctgggc ttacaggttg ggcacaagta aatggtggat
     4201 acgaaatcac tcctgaagaa aaacttaaag aagatattta ctatataaaa aataggagta
     4261 tattacttga ctttaagata ctctttaaaa ctgttaaagt agtattgaca ggagacggag
     4321 ctagataatt ttaaaatatt agattactaa tcaatgaaaa agctctttaa aaagagcttt
     4381 tttattgtca aaaaatacaa aaaagaaaag gaatgagaac ttatgattaa aaaaatttct
     4441 actatattat cgttagtttt gttaatctct atttctagta cgataggagt atttgcagat
     4501 gcaaatccca aaagagaatt gatagaaggg agtataccag agatttcgac agaattaaat
     4561 aaaagagcat ttaaggattc taaagaagta attcttgtaa atgaagagtc aattgtagat
     4621 agtataagtg caacaccttt agcatatgca aaaaatgctc caatagttgt aactaagagt
     4681 aaaaatcttg gtagagtaac aagaaattat ctaaaagaac taggtccaga gaaggttacg
     4741 atagtaggtg gtttaaaggc agtatctaaa gatgctgaac gtaatataga aaaaatggga
     4801 atgaaagtag aaagaataag agggaaagac agatatgata catctttaaa gatagctaga
     4861 gaaatgtata gaacagtagg atttgatgaa gcatttttac ttagctcaac aacaggatta
     4921 gagaacgcca tatctgtata ttcttatgcc gctaaaagtg gaatgccaat aatatgggct
     4981 aaagatgaag ggtttgagga acaaattgat ttcttaaaag gcaaaaatct taaaaaaata
     5041 tatgcattag gtgattcaaa agagtttatt gcagagattg attctaactt aaaaaatata
     5101 gaaggaataa agcaaataaa taagtcaagt acaaatgttg atttaataaa gaagttttat
     5161 gatgaaaaag atataaagaa aatctatact gctagattag actttggtag tagatctgat
     5221 gtaaacgaat atatttcttt gggagtagta tcagcaaaag aaaatatgcc aatactgatt
     5281 tgtagtgata acttaagtcg tgcacaagat aaattcttga aagatagcaa tataaatgat
     5341 gtagttgaag ttgggtatac agtaggtgat tatagtctat ttaagagcat atttaatcta
     5401 acattcttat cttgtatagt attaatacta ttattactat tgattacatt tagggcactt
     5461 agatatgagt cgaaatagtt tttgatttat aaggaagctt ttcataggtt aaatttactt
     5521 aattagaata atgtttataa gcagttttaa aagttctact tttagagttt aaattagata
     5581 aggagaaaga agatgtcaaa aacagctaaa gcagcattgt ggattatggc agccactatg
     5641 ttttcaaaag tattaggttt ccttagagaa cttgtacttg caaatttcta tgggacaggt
     5701 atgtatgcag acgtatttgt attaacatta aatatacctg gtttaatcat agcagtcata
     5761 ggctctgcag ttgctacaac ttatatacct atgtactttg agacaaagaa aagactcgga
     5821 gatgaaggtg ccttaaaatt tactaataat gttttaaata tatgctatat aatggctata
     5881 gtaatagcta ttattggtct tttatttaca gagcaatttg ttacagtatt tgcagcagga
     5941 tttagaaacg accctgctaa gttccaagca gcaatattat ttactaaaat aatgatttca
     6001 ggagttttat tcctgagtgg aagtaagata tttagttcgt ttttacaggt gaatgacagt
     6061 tttgtaattc ctgggcttat aggaatccca tataatatta taataatagc agcaatagca
     6121 ctaagtgcag gtaaaaatgt atggatactg ccagcaggag cattattggc tatggcgagt
     6181 cagctattgt ttcaactgcc atttgcgttt aagaagtcct acaagtataa gccatatata
     6241 aatttaaagg atgagtctat aaaagagtta gtcaacctag tattgcctat gttggtagga
     6301 gtagctgttg gacagttaaa tatatttgtt gaccgattat tggcaaccac tttgggtgat
     6361 ggaaagttgt ctgctcttaa ctatgcaaat aggctaaatg agtttgttat ggcattattt
     6421 gttacatcta taataacagt tatatatcct aaactagcaa aaatgtctgg taaagataat
     6481 aaagaaggat ttataagcac tattgtaaaa tcttcaaatt gtattatatt ggtggtgctt
     6541 cctatatcta taggagctat tatattagca gagcctcttg ttagaatatt attccaaagg
     6601 ggtaaatttg atgcgttatc aacagacctt acatctatag cgcttagatt atattcacta
     6661 ggtttattgg cttgtggagt aagagatgtc ttatataggg cattttactc tctttcagat
     6721 acaaaaacac caatgataaa tggaagtata gctttaataa taaatatagt tcttaattta
     6781 atattgataa gaccattggg tcatgcaggt attgctatat caactagtac atcaaacata
     6841 attacagtta tattattatt tatatctctt aaaaagaaaa atggatattt tggaggggat
     6901 aaaattataa agactggtct aaaaagtttg gtagcatctg gtgtgatggc agtagctact
     6961 ttattgatat ataataatct atatgcattt atgggttcag gaacaattaa agaaattata
     7021 tcagttggag ctggagtatt aggtggagct tctgtatata ctgtattgat agttttattt
     7081 aaggtagaag agatggattt agcttttgaa tttttaagaa aaggaaaaca aaaactactt
     7141 agaagataga taattacctt gaaattttaa attagagtca gaaaggatga aaatatggat
     7201 tataagaata attatgagat gtggttaaat tctccttatt ttgatgaaca aacgaaaaat
     7261 gagctattaa gtataaaaga tgatgaaaaa gaaatacaag atagatttta taaaaattta
     7321 gagtttggaa caggtggttt aagaggtata ataggagcgg gaactaatag aatcaatatt
     7381 tatactgtta gacgtgcgac actgggcgtt ttaaattata tcatgaaaac tcaaggagaa
     7441 gaaggtaagc aaaagggaat agttatagca catgatagta gatacatgtc tagggagttt
     7501 tgtatagaag tagcaaagac tttaagtgct tatggtgtaa aggcttatat ttttgaagaa
     7561 ttaaagccta caccagaact ttcttttgct gttagatatt taaagtgtgc tatgggaata
     7621 gtaataacag caagtcataa tcctaaagaa tacaatggat ataaagtata tgactcagat
     7681 ggtggtcaaa tctgtataga tatggcaaat gatataatag cagaagttaa taagattgat
     7741 gattacagta ctattaaaag tatagatttt gaagaagctt tatctaaaaa tttaataact
     7801 atattagata atgaagtaga tgatgagttt ataaaagctg ttaagaaaca agttttaaga
     7861 caaaatataa tagatgaata cgggaaaaaa ttaaaaataa tatatacacc tattcatggt
     7921 acaggaaata agccagtaag aaaagtctta aatgaatgtg gttttgaaaa tgtaatggta
     7981 gttaaggagc aagaattgcc agattctaat ttttcaacag taaagtatcc taatcctgaa
     8041 gaaaagtcag tgtttaatat agctattgaa atggcaaaga ataatggcac tgatttgata
     8101 ataggaacag accctgattg tgatagagta ggtatcgttg taaaagattc aagtggtgaa
     8161 tatgtagtct taaatggaaa tcaagtcggt tcacttttag ttagatatat attagagagt
     8221 ttagttgaag aaaataaact tcctaaaaac aatcctacaa taataaaaac tatagttaca
     8281 tcagaactag gtgcaaaaat agcaaaggct tataatgtag attgtttaaa tactttaaca
     8341 ggatttaaat ttataggaga aaaaataaaa gcgtttgaag aaagcaatga tagaagtttt
     8401 ataatgggat atgaggaaag ctatggttac ttaattggaa ctcatgctag agataaggat
     8461 ggtgttgtat cttcacttat gatatgtgaa atggcagcat actattcttc aaaaggtatg
     8521 aacttgtatg aagctttgat agatacatat aataaatttg gatactacaa agaggactta
     8581 aagtcagtaa ctttaaaagg catagatgga ataaaaaaaa taaaagagat gatgctttac
     8641 tttagaagtg ttaaaataga taatgtggct gatgtaaaag tagataaaat tttagactat
     8701 aaagatggtg ttgatgattt acctaagtca gatgtactta aatttttact tgaagatggc
     8761 tcttggatag ctataagacc ttcaggtaca gaacctaaaa ttaaatttta ctttggagca
     8821 aattcagaca atcaagaaga tgtagaattt aagttgaaca atttaatatc ttatatatta
     8881 aatgtagtgg attctatata aaaagctaag gggagataaa caatgaaagt atacaatgtc
     8941 ataatggctg gaggaggagg aactcgtttc tggccactta gtagacaaga agtacctaaa
     9001 cagcttataa atttaagtgg agaagatgct ttaataaacg aaactataaa cagaatagat
     9061 tctctagcaa aaaaagatga tttatttata gtaaccaatg agaagcaatt ggaagcttta
     9121 aaggatatag taaaagataa atgtttggat agtaatatat tgccagagcc atgtgccaga
     9181 aatacagcag cagctatagg ctttgcagca tttaacataa tgaaaaaata tggtgatggt
     9241 gttatgtgtg tatatccagc tgaccattat ataaaagatg aaaaagaatt taaatctatt
     9301 ttggaaaaag ctatctatat agcagaaaat aatgataaac tagtaactat tgggataact
     9361 cctacatttc catcaacagg atatggatac ataaatttta atagagaaaa tactatagaa
     9421 gatgttgcat atgaggtagt ggagtttgta gaaaaaccaa actatgaaat tgctaaagaa
     9481 tatgtaaact ctaaaaagta tgtatggaat agtggaatgt tcgtatggaa ggtatctaaa
     9541 atactagaag attttaagag atacttgcca aaagtatatg aaaaattaga agatataagt
     9601 aaatacttag gaacaaaaga agaaatggaa aaaataaaag aaatataccc aactatacaa
     9661 tcaatctcta tagattatgg tattatggaa agaagtaatg atgttatagt agttcctggt
     9721 gattttggat ggaatgatgt tggttcttgg gattctctag gtgcaatata tccaactgat
     9781 gatgaaggta atatcaaaag aggtgaaaat attactatag atactaaaaa ttcaattata
     9841 tattcagatg acaaattaat atcaacaata ggtattagtg atttgatagt agtatctaca
     9901 aatgatgcag ttatggtttg tagaaaagat aaagctcaag atgtcaaaaa aatagtagag
     9961 cagttaaaag aagaagatag acaagaatat atgtaggagg tgttcatttg attcctaaaa
    10021 aaatacatta tgtatggttt ggtggtccaa aaggaaatat agaaaatata tgtataaatt
    10081 cttggaaaga aaagcttcca gaatatgaga tagtagagtg gaatgaaaaa aactttgata
    10141 tagagaaaga aataaaagga aataagtttt tggaagaatg ttataaaaga aaactttggg
    10201 cattcatatc tgattataca agaataaaag tactctatga acaaggtgga gtttatatgg
    10261 atacagatat gcaaatatta aaggatataa ctcctctttt agaaaataat aggctagtct
    10321 gtgggtatga agatgataga gagtatataa atggtgctat aataggtgta gaaaaaggac
    10381 atcctttttt aaaagactta ttggagtatt atgaaaaaga agtacttact tcttcattgt
    10441 ttacaatacc aaagataatg actcatctta tggaaaagaa ttataagaag attgatccaa
    10501 ataattatga agaaggaata cgtgtttacg acaaagaata tttttatcct tttggattta
    10561 aagaagactt tacaccagaa tgtataacag aaaacacttt tgggatacat tggtggggaa
    10621 aaagttgggc taaaaagaga aattatttct tagaaagcaa acacttaaca ggtgtaaata
    10681 agatatggaa gtgttgtaaa atatttgcaa gtaatacttt acgaagctaa aagggagaaa
    10741 gtaaaatgaa gactttaaat gatgatagaa atattatgat tagaaaatat ctttatatat
    10801 ttgttttgtt tatcatatta cttagaaagt atatgatatc atttattgac ccaaatatgg
    10861 atatagggat gattaaatca gcgttatttt attcttcaat aggaatacta atgctacttt
    10921 ttttatttga taagagaaaa agtataacag aaatggtact agttggagtt tgtgtcttac
    10981 tttatttatt aaatagggaa ggagctatat tattaatcgt tttattagct gtatcagcta
    11041 agcaaataga tgataaattt atagttaaaa attatttaat aatatcggca tgttttttaa
    11101 tggtagctat attattattt aatttatttc cttcattaat atttaatcag gaagtccact
    11161 atagatatat agaaaaaata gatatgcttg ttacaagaat ggattttgga cttggtaatc
    11221 caaatagtgt attttatcat atggttacta tatatgcagc atatatattc ttaagatata
    11281 aagattataa taaatgggat agaatacttt tatttggttc tgcatttttt gtttatcaaa
    11341 ccacatatag tagaactgga tttttcacta tattagcagg attaatattt gtagaaataa
    11401 taagatggat agatataaaa aaaataaaag gactaccaat gctcctaaag acattaccgg
    11461 taatacttac tttatttagt gtaattattg gtacagtatt tgataaaagc actttattaa
    11521 atagattatt agcaagtaga cctaagttct ggaatgtata cttggctgaa aaggggaact
    11581 ttctaaaacc ttttggaaat tcatatagtc ctgctataaa agcaactaat ccattagata
    11641 gttcttatgt atatataatt agtgtactag gagtagttgc ttgtatatta tttatgtatt
    11701 tgatgtataa aggaatagaa agctttatag aaaaagataa aaaagcatat ttagtagctt
    11761 tatttatatt tttactatac tcttttgcag aaaatttatt attagaagct tcatttagtt
    11821 ttgcagtagt attactaata aaagaagtga tattaaatga taaaagggaa atagatttgt
    11881 ggaagatgaa aagtaggagg tagtaatgtt aatatcatta ataatgccaa cattaaatag
    11941 atatgatgat atatacttac ttatggacag cttagaaaat caaacttaca aaaactttga
    12001 acttatagta gtagaccaaa atgataatag caaagttaaa gaaatagttg ataaatacat
    12061 tgataagtta gatataaagt atataaaaag ctctaaaaaa ggattgagtt ataacagaaa
    12121 tgtaggcata gacaatgctg tagggcaaat tataggtttc cctgatgatg attgtgtcta
    12181 cgaaaatgat acattagaaa aagttataaa tttctttaat aaaaataaag attataaaat
    12241 atacagttgc aaaactatgg attcaaataa agttgatgca tttaagaaaa tgtatgatgg
    12301 aacttgtgat atcacaagtt ccaacgtttt ggatactata acatccataa ctttttttat
    12361 tgattttgaa ggtaaagact atacaagatt tgatgaaaaa ttgggtgttg gtggagagtt
    12421 tggtgctggt gaagagatag attatgtttt aaacttattg agtttaggtt ttaaaggaaa
    12481 atactttgga aatgatataa tttaccatcc tgcaaaaaaa cattctaaat ctaaagaaaa
    12541 gtatcaaaaa gattataact atggaagagg ctttggagcg ctttgcaaaa aggaaatagt
    12601 ctatagaaaa aattataagt ttgcaaaagt tatggtatca aaacttgtaa gaaatatagg
    12661 aggtcttata ttgagttcca atagagatta tcatagtgcc actataaaag gaagaattaa
    12721 tggatttaga cagtacaagt tataatgagg atggtgttta tggacaaggt taaattttta
    12781 ataaatatgc ttttggcttt ttttatatat ccatttaata agcataagtt taatggtaga
    12841 gatatctggc ttataggtgg acattcaggg gatatataca atgataattc aaagtttttt
    12901 tatgagtata tgctaaaaga acataatgat gtagaaactt attgggtagt aaataaagac
    12961 agtaaagtat ttgataaaat tccaggcaaa aaattaataa gaggaagtgt agaaaactat
    13021 ctgtattatt acaattcaaa agctatagta ttttcacatg caccatcagc agatatagca
    13081 ccatacaatt ttgctgtacc tgttcttaat tattttcata aaaaaacgat aaaagtgttt
    13141 ttaaatcatg ggactataag ttttaaaaaa agaaaaccta tgaacaaaaa gttcaaaaat
    13201 attatagata acctatataa aagctataat atagttacag caagttctga atttgaaaga
    13261 aatgttatgg taaatgattg gggaatgtta gatgactcag tttatataat tggaaatgca
    13321 agatatgaca atttaccaac aaatgaagtt gcacaaactc gagatatatt atatactcct
    13381 acatggagag attggataaa atttagttca ggaaaattta cggatacaga ttattttaaa
    13441 aatataatga attttttaaa tgatgacaaa ttaaataaaa tattagatga gaaagatata
    13501 aatgtaaaaa tttatatgca tcatcttatg catgagttta tagatgatat aaaagaaaat
    13561 ataacaggaa aacgaattgt gtttttagat aaaggggtaa ctctagcaaa tgaaatcaga
    13621 aaatcagctg caaatataac tgattattca agtgtagcca tagacttttt gtatatgaat
    13681 agacctatct tgttttacca attcgatttg gatgaatata tggaaaaagt tgattcatat
    13741 atagatttaa aaagtgaaat gtttgggtct ttggcttata ataatgatga agccgtgaat
    13801 aaacttattg atattattga aaataatttt gaggttatgg ataaccaaaa aaatgaaaga
    13861 aataagtttt tcagatataa tgataataag aactgcaaga ggatatatga ttgtgtatta
    13921 agtaaaatta aataaatatt ttataaatga tgaggaaggt aatatgaaga agaatttagt
    13981 atctataatt actcccatgt ataattctga aaaatttatt gaagcaacca taaaatcagt
    14041 attaaaccaa acttatcaag aatgggaaat gttaattatt gatgattgct caacagataa
    14101 tagtcctaat atagtcaaat cttatatgca acaggatagt agaataaaat gtataaagac
    14161 tgagactaat aagggtgtct ctaatgctag aaatttagca ctaagtaagg caacaggaca
    14221 atttatagct tttttagata gtgatgacca atggaatagt agtaagttag aaaaacaagt
    14281 aaattttatg ttagaaaatg actatgtaat ttcatttact tcatatgaac tgatggatga
    14341 aaatgataaa aaattaaaca aagtaataaa agttccacct aatgtagatt atagaagatt
    14401 attaaaagga aatatcttag gatgtcttac tgttgtgatt gataagtcta aattggattt
    14461 tgaaataaga atgagtggtg taagacatga agattatgtt ttatggttat cgatattgaa
    14521 aaaagggcat attgctcatg gaataaatga agttctagca ttatatagaa aatctagcaa
    14581 ttccctaagt ggtaataaaa taaaagctgc tatgtggact tggaatatat atagaaatat
    14641 agaaaaaata cctttataca aagctatata ctactttatt aattatggaa taaacggaat
    14701 caaaaaaagt tagacagaga ggaagattta attaatgtat aaatataaat ttactgtatt
    14761 tactccgacg tataacagag ctcatttact tgaaaatttg tacaatgatt taaaagctca
    14821 aacttatgat ttcaatgatt ttgaatggtt gattgttgat gatggaagta gcgacagtac
    14881 taaagagtta gttgaaaaat ttatatcaga aaataaatta aatataagat atatatacaa
    14941 agaaaatgga ggaaaacata ctgcaataaa tctaggtgta aaaaatgcag atggagagct
    15001 atttttcatt gtagatagtg atgatggttt aattaaagat tctcttgaaa ttgctaatga
    15061 tgagtgggat tctttagaga ataaagaagg atttagtgga gttgttggac tttgtgtata
    15121 tccaagtgga gatttgatag ggacagaaat gcctgaagat aagaaaatat gtcactttgc
    15181 agacctatat tttaagtatg gcgttaaggg agataaaagc atagtatttg taactgatga
    15241 gcttataaaa tttccttttc cagaaagaaa agaagtgaga tttttaccag aaagtgtagt
    15301 atggaatgaa atgtctaaat actataaagt tatatgtgta aataaaccta tgataataag
    15361 agattatttg gatgatggtt tgactaagaa tatattaagt aaaaatgcat taagaggtag
    15421 agcattagag tttttatatt taataaatca aaatacatat ccattaagta gatatccata
    15481 tatgtggata aaaaattaca taaatttagc cagatactca ttgttgtctg atagtcatta
    15541 ttttggtgaa ttaagaaaag tatctgataa attgttgtat ttagcattat tcccattagg
    15601 atattacaaa tatattggac aaagaaaatt agtatctaag taaacttgtt ggtatagtac
    15661 tgctagttgc tatataagga gagtaaaaat atgaatgagc ctttagtatc gataattact
    15721 cctgtttata attcagaaga gtttttatca gagacgataa agtcaatcca aaatcaaact
    15781 tataaaaatt ggcagctttt attagtggat gattgttcta aggacaattc aagtgaaatt
    15841 ataaaaagtt ttcgaaaaga agatgctaga ataaaatata taaagcttga aaaaaattct
    15901 ggagctgcag tatctagaaa tgttggtatt aagaatgcgg aaggtcgatt tattgcattt
    15961 gtagatagtg atgatttatg ggatagtaga aaattagaga ttcagataga atatatgtta
    16021 aaagaaaatg taggtttttc ttttacatct tatagatata tgagacaaga tgggtcaaaa
    16081 acgaataagg ttgcaagagc tcctaagaaa attgactatg aaggattact tagaaatact
    16141 ataataggat gttctacagt ggtcattgat aaggagattg taggagagtt cagcatgcct
    16201 ttggtaagaa gaggacaaga tactgcaacc tggttacagt tacttaaaaa ggaaaaatat
    16261 gcatatggga ttcaagaaga tttagtgaat tacagattag ttggaaattc tatatcaagt
    16321 aacaagataa aagcattaaa acgaacttgg aatacttata gaaatgttga aaatttaagt
    16381 ttaccaaaaa gtctatatgt attttgtttc tatgttttta atgccattaa aaaaagagta
    16441 taagttttat atacttggag gtaaaaaaat gaaaatagct atagctggga cgggatatgt
    16501 aggtttggtt acaggtgttt gtctagcaga ggtaggcaat gaaaatgtag tctgtgttga
    16561 tgtagatgaa caaaaagtta aatcaatgaa acttggaata gcacctatat atgaagaagg
    16621 cctagaagaa cttatggtta aaaattacaa cagaggaaga atagattaca ctacagacta
    16681 taaaaaagct tatgaagatg tagatgtaat attaatagct gtaggtacac ctgagagaag
    16741 tgatggttca gccaacctag attatataaa ggcagttgca aagcaaatag ctgaaactgt
    16801 gacaaaagat actctagtag taataaaatc tacagttcca attggtacaa atgaagagat
    16861 agaagattat ataaatagaa acctaaaaaa tgatgtaaaa gtagaacttg catctaatcc
    16921 agagttttta tcacaaggaa cagcagtaaa agatactcta catgcttcaa gaatcgttat
    16981 aggtgtagaa agtgaatggg ctagagaaat attagaggaa atctataagc cttttaatca
    17041 acctataata gttacaaata gaaatagtgc tgagatgata aaatacgcat caaataactt
    17101 cttagctctt aaaatatcat ttatgaatga tattgcaaat ctttgtgaaa tagcaggagc
    17161 taacatagaa gatgtagctt taggaatgag ttatgatgat agaataggaa gtaagttttt
    17221 aaaggcggga ataggatatg gtggttcttg ctttccaaaa gacacaaaag ctcttcactg
    17281 gttatctgaa cataatggtt atgaattaaa aacagtaaaa gctgcaatag aagttaatga
    17341 aagtcaaaaa cttaaattag ttagaaaggc atctaagtta gtagagagct ttcaagatat
    17401 gaaggttgct gttttaggtt tgacttttaa gccagacact gatgatataa gagaagcacc
    17461 atctatacca aatataaaat atttgctcga aagaggggca agagttactg tatacgaccc
    17521 aattggtgta aataatacta agaaaatatt tggtgactct atactttatg ctgattctgt
    17581 agatgatgcg atagaagatg cagagatatg ttttataatg acagaatgga aagatgtatt
    17641 aaattatgat atacataagt ataaggaaaa gatgaaaaat cctttagtcc ttgatggaag
    17701 aaattgttat aaaattaaag atatggaaga gttaggaatt gagtattatt cgataggaag
    17761 gtaaatatta tgaagactgt ttgttattta gctgatgggt caaatcctca tactttaaaa
    17821 tggtgtagtt tttttaaaaa taaaggatat gacattcatg taatctcttt aaatggaggt
    17881 cacatggaag gaataaaaat ttataatttt ggttctaatg tagaagaact aaaaaatgaa
    17941 aatatttcta aaaaaacggg atacttaggt tcaataaagc agataaagaa gctagtaaat
    18001 gaaataaagc cagatatatt gcatgcacag tatgcaagta gttatggatt tataggaagt
    18061 ctacttgggt atcatccata tattatttct gtttggggta ctgatattta tgatttccct
    18121 aataatggtt ttatacaaaa acaaattata aaacataact ttagaaaagc agactatatc
    18181 ttttcaaata gtagagatat ggctaaagag gctaataaat atacagctaa acatgtagat
    18241 gtaacctttt ttggagtaga tatggatagg tttaagccta tggaagtaga aaaagaagac
    18301 gcttttgtaa taggaattat aaagagttta gaaaaaaaat atggaataga atatttaata
    18361 caagcattta agatgcttaa agatgaatat aaagataaga aaataattct taaaataggt
    18421 ggctctggaa gtcagatgga caacctaata aatttagcta aagaattggg tatagaaaat
    18481 gatgttcagt ttttaggaag aatatctcca gaaaatgtaa gcaagacttt taattctttt
    18541 gatgtaactg tatttccttc tttaagagag ggatttggag tggcagctat agaatcagaa
    18601 gcatgtgaag tacctgttat agttacaaat gtaggaggac atccagaatc tgtatgggaa
    18661 aatgaaactg gcttaattgt agaaccaaaa caaccagaag aaataaaaaa tgctataata
    18721 aaactaatgg aaaatgatga acttagactt aatatgggta agaaagggcg tcaatttgtt
    18781 agagaaaatt atgaggttaa tttaaacttt aatgatatag aaaaaattta tgattctata
    18841 tttgataaat ataaaaaata aaatgaaaaa atacagcaaa agatagatgt tgttggctat
    18901 gcttaggtgc gtagcttttg ctgtttaaag gagggcttct agttatgcga gaaagtatag
    18961 atttagagaa agaaagcttt tttaataggc tttttaaaaa atataaattt agacatttaa
    19021 cactattaat tctagatatt tgtagcgtac tagtagcgtt tcgaatgtct ttaagcttga
    19081 caggtaattt aaatgcattt accactaatg atataataat ttcagtgtgt atatatatag
    19141 tacttcatat agtatcattt agattgttta agtgctacaa cacattatgg agatatgcag
    19201 gagaagaaga aattatatct atttttgtgg caactttagc ttatttaatt ccgatttata
    19261 taataaataa attattaggg tttgattatc ctataatgtt ttatgtatta aatacaatat
    19321 ttattataat gtttacaagt ggcgcaagaa tagcctatag agctataaga atagtcatga
    19381 acaagacata ttctagagga aaggtttcaa atatccttat aattggtgca ggagatgcag
    19441 gagaaatggt tatacaggaa ctaaaaagaa atccagaact taaaaaagta gcagtggcta
    19501 taattgatga tgataaaaat aaaattggaa gaataatcca taatgtgaaa attgtaggaa
    19561 caacttcaga tataaaagat gtggtagaaa agtacaatgt agatgagata atcttttcaa
    19621 tagcaaacat agagaaacgt agaaaaaaag atattattga tatatgtaaa aacactaact
    19681 gtaaaataaa gacaatacca ggaatttacg aaattataga tggaaaagtt gatataaaac
    19741 agattagaga agtagagata gaagacttgt tgggtagaga gcctataaaa acaaatttaa
    19801 gagagataag taattacatt gagggaaaag taatcctaat aactggagga ggaggctcaa
    19861 ttggttcaga gctttgtaga cagattgcag gatttaatcc taaagagctt attatagttg
    19921 ataattatga aaataatgct tactcaatac aacaagaatt acttagaaaa tataaaaata
    19981 aattagattt gaagacagta atagcatcaa taagagaaga aaaaagaatg gatgaaatat
    20041 ttaataaata taaaccagaa gtggtatttc atgcagcagc acataaacat gttcctctta
    20101 tggaatcgag ccctggcgaa gctataaaga ataatatatt tggaacttta aacatagctg
    20161 gcttgtcttc aaaatataga gctaagaaat ttgtccttat atctacagat aaggctgtaa
    20221 accctactaa cataatggga gctactaaaa gagctgctga aatgattata cagactatga
    20281 atgctgaaag ccagactgaa tttgttgctg ttagatttgg aaatgtgctt ggtagtaatg
    20341 gtagtgtaat tccacttttt aagaagcaaa ttgaagatgg cggaccagtg acagttactc
    20401 atccagatat tattagatat tttatgacaa taccagaagc agtaggctta gttatacaag
    20461 ctggggctat ggctaaaggt ggagaaatat ttgtgcttga tatgggagaa cctgtaagaa
    20521 tattggattt agcaaaaaac ttaattaaat tcagtggatt tgagccagat gttgatataa
    20581 aaattgaatt ttctggtctt agaccaggag aaaagttgta tgaagaacta cttatgtcag
    20641 aagaaggtct attggataca gagcataaaa agatttttat tggtagacca atagatgttg
    20701 atagagaaaa aataacaaaa tacttaaaac ttttaagaga aattactaac aatgaagagg
    20761 ttgaaaaaat agatggtatt atgagggaat tagtaccaac ttatattaaa ccagaagatg
    20821 ctaatatcaa agaaattgca acaactagag aaaagtagaa tatatttcta atttgtaacc
    20881 agggggagca aaaagaaaca aatatatact aaaaagaaac aaaagattac aaaaagatta
    20941 caaaaagatt acaaaaaagg ttgcaattta ttttgatgta agatagaatg ttaattagga
    21001 attggaaacg aaggaacaaa atgttaaatt ttcgaaagga ttaggtattt gagattatga
    21061 tgaatattaa aaataaaaaa catatattga aaaaatttat agctatggtg ttaatagcag
    21121 gagttgtcac agtagaagca ggagcaataa cggctagtgc agcagagcca actaattcac
    21181 ctatgagtgc aacagtagac cagtgtgatt ttttaaatgt aagaagtgga gctagtgcaa
    21241 atgatgcagt agtaggaaag ataaacacag gagacaaagt tgaagtttta gaattacatt
    21301 caaatggatg gattaaaata aaaagtgttg acaatgttac aggatgggta aatggagatt
    21361 atttaactat tcaaggtgga aatgtagatg caaaggtaca aaatgtttta aacttagcat
    21421 ttaagcaaca aggtaagcca tataaatggg gagcaactgg accaaattct tttgattgtt
    21481 ctgggttcac ttcttatgtc tataaaaatg gagcaggagt taatttacca agagtatcaa
    21541 gatcacaagc aacagtagga aagaaagtat caagagcaga attaaaacca ggagatttag
    21601 ttttctttgg ttcaggtggt tcaataaatc acgttggatt gtatgtagga gattctaaat
    21661 tcatacattc accacaaaca ggagatgtag taaaagttac tagcatggct ccaggaacta
    21721 attatgcaaa aagacttata actgctacac gtgttttaca ataataatag atattgattt
    21781 acataggatt gaatattaac attaaatata taattacatt taatgtttta catagagatt
    21841 taagagttta gtaatattaa aatagtgatg aacgttataa aaacagcaaa gaagataaat
    21901 ttctttgctg ttttttttat aataaattat ttcatattat aatcaaatat caaatataaa
    21961 ttttaactat attattcaat atttgacaga taatatgaat tttagatata cttaataaga
    22021 agtatattgt agaaaaaata aatttaaaaa attaaggggg atgaaatgaa aaaaataagt
    22081 atattagtat tatcattgat aatgacactt actatgtgta gtgtaagttc atttgctgat
    22141 tcatcaaatg ataaggaaat gagagctgct tggatatcta cagtttataa tttagactgg
    22201 cctaagacta agaataatga agccaagcaa aaaaaggaat acacagattt attggacaaa
    22261 ttaaaaagtg taggaataaa tacagcagtg gttcaagtaa gaccaaaatc agatgcttta
    22321 tataaatcaa atataaaccc atggtctgaa tatcttaccg gtacacaagg taaagaccct
    22381 ggatatgacc cattaccatt tttaatagaa gaagcacata aaagaggaat ggaatttcat
    22441 gcatggttta atccatatag aataactatg gcagatgaga gcatagataa attgccagca
    22501 aaccatccag caaagaaaaa tccaagctgg gtagttaaac atggaaataa gtattactac
    22561 gacccaggtt taccagaagt tagaaagtac atagtggata gtatagcaga agtagttcaa
    22621 aattacgata tagatggagt tcattttgat gattacttct atccaggagt aagtttcaat
    22681 gatactgcaa cataccaaaa atatggaaaa ggacaaaata aggatgattg gagaagagaa
    22741 aatgtaaata ctctacttag agatgtaaaa gcgtctataa agagtataaa gcctaatgtt
    22801 gtatttggtg taagcccagc aggtatatgg agaaataaga gtagtgaccc aacaggttca
    22861 gatacatcag gtaatgaaag ttatgtaggt acatatgcag atacaagagc atggataaaa
    22921 caaggattga ttgactatgt agttccacaa ctttactggc caattggtct taaagcagca
    22981 gattattcta aattagtggc atggtgggct aatgaagtaa aaggaacaaa tgtagactta
    23041 tacataggac aaggtatata taagcaaggt caatcgagtt atggtggaca aaatatagca
    23101 aaagaaatag tacaacaagt aacattaaat agaaagtata gtgaaataaa aggtagtatg
    23161 tatttctcag ctaaagatat agcaaatagt acttctatac aaaaggattt aaagagttta
    23221 tattctagta gtgaagaacc agttactcca ccatctaatg ttaaagtgga gaaattaaga
    23281 ggcgacgaaa gatatgacac agcagtagca ataagtaaaa aaggttgggc tacaaattca
    23341 gatacagtag tattagtaaa tgggtactct atagttgatg gtataacatc aacaccactt
    23401 gctacatcaa atgatgctcc tatactattg gtaaataagg ataatatacc tacatccact
    23461 aagaatgagt taaaaagact taatccaagt aaagttattt taattggagg aaataactct
    23521 attggagata aagttgagag tgagataaaa gatacattgt caaatgtatc aataaataga
    23581 gttggtggtt cagatagata tagtacatct ttaatgatag caaaagaatt agtaaaaact
    23641 aatccagtag aaaaactata tataacaagt ggtacaggtg aagcagattc tctttctata
    23701 gcttcaaaag caggagaaga aaagcaacca atagtattag tatcaaaaga taatgtaagt
    23761 gatgaagttt acaattggat atcagattta aaagtaaaag atgcttattt tataggtggt
    23821 aatctaagta tatctgattc agtaataaat aagctggata aggtcattac aaatgatgta
    23881 tctaaaaata gaatagctgg agaaaataga gaagaaacaa atggaaaagt aatacaaaaa
    23941 ttctatccaa atgctgaata ttcatcaatg tttgtaagta aaagtaatca attggtagat
    24001 gcattaacat caggtccttt agcagctaaa ttaaaatcac cagtagttat gcttggaaat
    24061 agtgtaacat ctgctcagaa aactgcttta gagcctaaga aaactactct agtatacgaa
    24121 gctggagatg gtataaatca aaacacatta aacactttct taaacttagt taagtaataa
    24181 aaaagttaat ttaaaataca gagtatttta taaatgtaga ttaaaatgaa aattatcatg
    24241 ataaattaga aaataaaaaa gaactatatg cattttgtaa tcaagtgtac acagcaactt
    24301 gaaaataatt atgcgatata gttctttttt agtaattaat cttatacttt ttaataaact
    24361 ttatgttgaa ttttttattg tttaaactct atgtcattaa atataaaatc gtgtaatatg
    24421 tctaaagtat ctgggtcaaa tctaagaacc catccagctt ttccatatat accacctgta
    24481 ctatgaattt catcatcaat aggaaattca aattgcttaa tatttaagtc gcccattttt
    24541 aaaacttgag ctcctaaaga aagtattgca ttaggcttca tgttagtttt aacatatgga
    24601 agaactgcat ctaataaacc tggatattta gtaacaggta aagtttccgt tttcttgatt
    24661 atagcttgta taatctgtct ctgtctgcca tctctagcaa aggctgtatc gttgtgtctg
    24721 atacgtgcaa aagatagtgc ctgatatcca tttagagtct gctttccaga gtttctaatg
    24781 tattgtatgc tacctttatc atcactttta ttccatttat aagtttcagg aataaattta
    24841 tttagttctc gtatttctcc tttttcaaca gtaacctcaa cgccaccaat agcatcaatt
    24901 atatacataa atgattcgaa attaacacaa gcataatttt gaatatcaat attaaaattc
    24961 tcttcaattg tttgtattaa taaatcagct tgtccataag cataagcatg agtaagtttt
    25021 tgtttaccat gaccagggat gtctacatag ctatcacgag ctaatgatgt aagtttgata
    25081 tcattgtgtt tgttatcaat agtaagtatc atcatagcat ctgaccttga agaatcttca
    25141 tttggtctag catctgttcc cataagtagt atgttaatta tcccatcttc atttttatgg
    25201 tcattgttat ccaataaatc actacttatg ctcgagtcat gaatagcaga taatttataa
    25261 taaaaatatc catagacact tatagggaat attactacca agaaagccag tagtataaca
    25321 aatttcttta attttgacaa aagtacccct tctttcttct ttttcctgta tattttttaa
    25381 tagcatacac ttaattatat taaagtattg agatatattc aatacttgtt tcagttacaa
    25441 aaaaaagaca atgatataat ttataagaaa atttatcgac ctatatatat agttaaaagt
    25501 atatataata tattttttaa aagtaattta tattttaaaa taaaaacaca ccccataaat
    25561 acaaagtaat ttgcatttaa gaggtatgta tttatcatca aattattggt ttgttttgga
    25621 atttctaatc ttcaaccata atatctttaa atatgaaatc atgcattgca tctagttcat
    25681 attcctcaaa aggtattaca tagcctgcat tacctatttt tctaccattt tcagtagaaa
    25741 gaggaaactc catagatttc aaatttaaat ttcctatact taaaagttct tttcctaaag
    25801 atattatttc attaggattc atattagttt ttacatacgg aagtattgtg ttaactagat
    25861 taggatattt agtgactggt aaatccttaa caccatttat taaactctgt ataacacttc
    25921 tttgtcttct atctctttca tctgtactat cattttttct tatacgagaa aatgcaagtg
    25981 tctgatatcc atttaatttt tgctcaccag aatttcttat atatttaaga gaacctttat
    26041 tgtcattttt attccattta taagtttcag gaataaattt atttaattct cttatttcac
    26101 tcttatcaac agtaacagta actccaccta aagcatcaac tatatccata aaagagaaaa
    26161 agtcaactac ggcgtagttt tgtatatcaa gttggaagtt ttcttctata gtttctatta
    26221 ataagttagc ttgtccatat acgtaggcat gtgttaattt ttgttctcca cgtcctggta
    26281 tattaacata agtatctcta tttaatgaag ttagcttaag acttttattt ttattatcaa
    26341 tagtaagtat catcatggca tcagaacgag aaacttcttc tcctggtctt gcatctgtac
    26401 ctaacaataa tatgtttgtg atattttctt cgtttttata gtcattacta ctaagcgcct
    26461 ctgtatttat actatcatct tgtatagttt ttaatttagt aaaaacatat ccatatatac
    26521 taacaggaaa tgctataact aaaagggcta gaaatataac gaactttttg agtcctgaca
    26581 ataatatccc ctactttcta aattttttaa ttacacatag ttaattatat taaaaatttg
    26641 ttacataagc aataaaaaac acaataaatt agcataagtt tgtcataata taattatttt
    26701 tatagcattt tatagaagaa agtgtgtagt tattattgat tttattggaa tataattgta
    26761 tgaagactta aatttttata aactgatgtg tatattgaaa aaaatatttt cctgaatgta
    26821 gttatagcat cttatgaaag gtgcaaattg taatttaaaa tttttagtgt ataataagaa
    26881 ttgaaaattc aaaccgataa aaattaataa aaaataatta taaattaata atttggggga
    26941 ggaaatgtat gataaaatta atagcaacag atttagatgg aactttgtta gatgaaaaga
    27001 gcgaaataaa tccagagttt tataaagtat ttaaaaaatt aagagagaga gggataatgt
    27061 tttctgctgc tagtggcagg cagtatcaaa atcttataaa aaaatttgaa gatataaaag
    27121 atgatatgat gtttatatca gaaaatggaa cactagtagt atataaagga aaagaaatat
    27181 tatcaaatcc tttaaataaa gaattagtaa atgaaatcat agagacaaca agaagtataa
    27241 aagggaaaaa aattgtaatg agtgggaaaa aatacgctta tatagaaagc aaagatgaag
    27301 cgtttataca agaggtcagt acttattatg caaagtttaa aattgtagaa gatttgacga
    27361 aagtagaagg agacatattg aagatagctg tatttgactt caaaggtgca gaacataata
    27421 ataatatcta ctttgaaaag ttttctgata gagctcaagt atgtatttct ggagtggagt
    27481 ggcttgattt aactgcaaaa ggtgcaaata aagggagtgc cataaaaaaa gtacaaaaaa
    27541 tgttagatat aaaatatgaa gaaactatgg tatttggtga ccagttaaat gatgtggaaa
    27601 tgatgaaaag tgcctatcat agttatgcca tggaaaatgc aaatgaacat ttgaaacaaa
    27661 ttgctagatt tagagcaaaa agaaatactg aaaatggcgt tgtagataag ataaaagagg
    27721 ttataaaaat tggatagtca tatgtataga tgaaaaagat tattttgaag aaatcaaaaa
    27781 aatatcaatg taattttgat aaataagttt atttatgcga aaattacatt gatatttaat
    27841 aaacatcaat aatagatatt tattttttta tgatattcag tattttgttt tctatttgag
    27901 ttgcattttc agcagaaatt aaataaacat atctacctgc cttctcaatt atagtattat
    27961 ttgctatttc agaattgatt ttttctccat gtccttctcc aaaaggtgct tgtatcatat
    28021 cttttttata attttctaga gttttataga ttttatctac ctcgtcagtt tttataacga
    28081 taatatcttc tagagataat ttttcagaag aattaattac aaaaccttga attatgtctt
    28141 cctctacatc attaaaatag ttaaaatcct ttgcatcaaa gatttttgat tcctttataa
    28201 gtaaatcaga agattttaat gcttttttta tttttgatgt agaaacgttt ttgtaatcac
    28261 cacttgaaga acaactaacc attcctaatg ataagattat cgaaaaaaac aatattaaaa
    28321 tttttttcat gataaacctc caagtttttt aaactctgtc ttaaattagt ttactacata
    28381 attatataaa aataaatgta tttaatgtta cagttttgat taataatgta atattttgat
    28441 aataatacct attgagaggt gattagaaat gagaatacca aaacatgtag gaattatacc
    28501 ggatggaaat agaaggtggg caagtcaaaa tggtatgaca aaagataaag gatatgaaag
    28561 tggaatagac cctggactag agtcttatag attatgtaga gatttaggct gtgaagaggt
    28621 gactttttat ggatttacaa ctgataatac aagacgtcca tcagtgcaga caaaagcttt
    28681 tacaaaagca tgtattgatg ctgcaaatat attaatagat gaaggtgctg ctttattagt
    28741 tgtaggaaat acaaaatctc caatgtttcc agaagaatta ttaccatata ctaatagaat
    28801 taaagccaaa gaaggtcaaa taaaagcaaa tcttctggta aattatggtt ggtattggga
    28861 cttaaataat atatcaggag tagaaaatgt aaataaaaat aatatacagg attacataca
    28921 gtcatttgat gtatccagac tagacttgat aatcagatgg ggtggaagaa ggagattaag
    28981 tggtttcctt cctgtacaat caatttatgc agatttttat gttatagata attattggcc
    29041 agattttaag agtgacgata taaaggacgc atttagttgg tatgatacac aagatgtcac
    29101 tttaggtgga taattaaaaa tattctgatt taaggttgta gagtgtttta aattattgaa
    29161 aaaaaaactt cttgatggta aaataacact aataatatgt aaaagtgtaa aaatttatac
    29221 caagaagggt gaagaaatgt caaaaggcaa taataacaat aattcaagaa acaaatctaa
    29281 aaagaccagt catctgaata gaaaaaagcg aaaattaaat aagaaaaagt tagcagtgtt
    29341 gatttgcttt acagttttat ttttatttat tgcatttaag gctactcaag gtgtagtagc
    29401 tttggtaaaa agtatggata aaagtaataa aactaatcaa caacaaaatg ttaactcaga
    29461 acaatttgac cttggtaatg aagaggaaaa taaaaagaaa aaatatactg tatttataga
    29521 cccaggtcat ggaggcaatg ataaaggaac tgaaagcaag accagtaata gatatgaaaa
    29581 agacctaaat ctacaaattg caaaaaaatt agcaaataaa ttatctaaac aaaaggatat
    29641 tcaagtagta gtatccagaa ctgatgatac atatatatca ttaaaggata gagctatctt
    29701 agcaaataat agttctgctg atgtgcttgt ttctattcat ttaaatgcag agaaaaatgg
    29761 taatacagca acaggtatag aaacttggta taggaataaa gctacagatg gttcaaaaga
    29821 acttgcacag acagtacaaa gtactatagt atcctatgtt aaagttagag ataggggaat
    29881 agttgaaaat aactttgaag tacttagaga atcaaatatg cctgctatat taatagagtg
    29941 tggtttttta acaacacctt cagaggaaca aaaaataata aatgaaaaat atcaagacca
    30001 acttgcggaa ggaattgttc aaggtgtact ttcataccta gattctaaag ggaataagta
    30061 agttaacttt actttaatgt gaggataaat ttaaaagttt aactgtgtaa attgtattaa
    30121 ctttatttcg taatgttgca aacattgttt gaatttagaa actatgaaat tagaaattat
    30181 tttatctata tctaatttca tagttttttt tgcaaaaaat ttatagttat aaaattttaa
    30241 aaaataaatt tagtataatg taatgtagtt atttatatgt gattttaact tgcattgatt
    30301 tgctatcaat tgtgctttat aattagtata tataaagttt aactgtaaga taatttaaaa
    30361 aataggggaa tttatatgaa taaaaatagc atgacaagaa tttacagttt aaataaaata
    30421 atttttatta taattggaat tttaggaata gttttactca caagacatat tgaggtagat
    30481 attaaagttt taattttaat tttattaacc ttgtttagta taaatgtatt tatttcatat
    30541 cttaaaatta agttgtatga ggataaaatt aatttaggat tgataaataa ttcagataag
    30601 tttataaata ttactaaaga tgagaacttc ctaaaacatg ttcaaaatta tattatcagt
    30661 aatgaaaaag aaaactgtgt aatggcttgt tttgatttat gtagactcaa acttataaat
    30721 gatatatatg gttatgagct aggggatgaa gtgttaaata atattttaag taacttaaaa
    30781 aattattttg gaaaagaagc tatttatgga aagttaaaaa gtgatgtttt tgtattaata
    30841 ataaagcttg aaaataggga acagaagatt ccttatttag taaatcttat taagaataaa
    30901 attgtgatac taagtcagat tgacagtttt aaaatggata taaatattga ggcttctatt
    30961 ggaatttata aatttaacaa taatgaaatt gatataaaaa aagctataga taatgctgat
    31021 atggcaagac ttaaatcaaa aggactaaag cacatagagt atgtagaatt tgataatgct
    31081 atggaagaag aaatacaatc aattatgaaa atagagagag atttattctt ggctattaaa
    31141 aataaagagt ttgtaattca ctatcaacct aaagttgatt caagtacagg taacattata
    31201 ggttcagagg cattgataag atggttgcac ccatcattag gcatggttgg accaaataag
    31261 tttataccta tagctgaaaa aaatggtctg ataaacaata ttggtagatg gctaattcaa
    31321 gaagttttta ttaccattaa caagtggatt tcagaaggta taaatgtctt accagtctca
    31381 ataaatcttt ctagagtaga actatataaa aatgatttag ttgatttttt taaactgatg
    31441 tttaatacat ataatattcc aaaagaactt atagaattag aaattacaga aacgacagca
    31501 ctaagagatg taaaatttat aagtgaaagg ctttatgaga ttaaatcttt aggtatgaat
    31561 atatcattag atgattttgg agtaggtaat tcaaatttta tcaaccttaa ggggatacca
    31621 ctagatatta taaaaattga taggtcactt atattggata tagttacaaa tacaaaaaca
    31681 aagtttatgg tgaaagcaat tgttaacctt tcacatgatt taaacgtaac tgttatatgt
    31741 gaaggggtag aggatatgca tcaggtcaaa gtcttaagtg aactgggatg taatttcata
    31801 caaggttatg tattttttaa acctttggat gagatgaatt ataaaaaact tcttactgac
    31861 ggaagttata taaatctgaa agatactctt ttagataaat gcatgacagt tgaggaggag
    31921 taaggatgat ttcagaagat gtagattatt caataagaat cataagagct atatttaata
    31981 atggagaaga taaaccaata gatgctaaaa tgatattaaa aagtgaagat ataccaatac
    32041 taacactata cagactgctt agaaagcttt gtgcaaacaa tattcttact tttaacaaag
    32101 aagatggcaa gacatacatg ttaaatgtgt cattggatac aataagtcta agaactattg
    32161 tagaagtaat tgatggaaaa atatatataa ataaatgtac gagatatgta tctaattgca
    32221 gtaattgctt aaaaggttgt aaagtaaatc gtgaattgct agaaatagaa aaagaaattt
    32281 ataaactttt atcgagaaaa tctataagac aaatactaga ataaaaaatt ttcagctatg
    32341 ataataattg gaggttttcg gttatggatg atatatctca agacaatttt ctactgtcta
    32401 aagagtatga gaatagctta gatgtagata ctaaaaaagc aagtggaata tattatactc
    32461 ctaaaataat tgtagattat attgtaaaaa aaactttaaa aaatcacgat ataattaaaa
    32521 atccatatcc acgaatactt gatatttcct gtgggtgtgg aaattttctt ttagaagtat
    32581 atgatatttt atatgactta tttgaggaaa atatatatga attgaaaaag aagtatgatg
    32641 aaaattattg gactgttgat aacatacacc gtcatatatt aaactattgt atatatggtg
    32701 ctgatattga tgaaaaagct ataagtatat taaaagatag cttaacaaat aaaaaggtag
    32761 taaatgattt agatgaatct gatataaaga ttaatctatt ttgttgtgat agtcttaaaa
    32821 agaaatggcg atataagttt gattacatag tgggtaatcc accctatatt ggtcataaaa
    32881 aacttgaaaa gaaatataaa aaatttctcc tagaaaaata tagtgaagtt tataaggata
    32941 aagctgactt atatttttgt ttttataaaa agattataga tattttaaag caaggaggga
    33001 ttggcagtgt tataactcca agatattttt tagaaagtct atcaggtaaa gatttaagag
    33061 aatatattaa atcaaatgtc aatgttcaag aaatagtgga ttttttaggt gcaaatatat
    33121 ttaaaaatat aggggtatct agttgtattc taacttttga taagaaaaaa acaaaagaga
    33181 catatataga tgtatttaaa atcaaaaatg aggatatatg tataaataaa tttgaaacat
    33241 tggaagaact tctaaagagt agcaaatttg aacattttaa tataaatcag agattgctat
    33301 ctgacgaatg gatacttgta aataaagatg atgaaacttt ttataataag atacaagaaa
    33361 aatgtaaata tagcttggaa gatatagcaa taagttttca aggaataatt acagggtgtg
    33421 ataaagcgtt tattttaagt aaagatgatg taaagttgaa ccttgtagat gataaatttt
    33481 taaaatgttg gataaaaagt aagaatataa ataaatatat agtagataaa agtgaatata
    33541 ggctaatata ctcaaatgat atagataatg agaatacaaa taaaaggata ctagatgaaa
    33601 ttatagggct atataaaaca aaattagaaa acagaagaga atgtaaaagt ggaataagga
    33661 aatggtatga acttcaatgg ggtagagaaa agcttttctt tgaaagaaaa aagataatgt
    33721 atccttataa atcaaatgaa aatagatttg ctatagatta tgataataat ttttcaagtg
    33781 cagatgtata ttcttttttt ataaaagaag agtatttaga taaattttca tatgaatatc
    33841 tagttggaat tttaaattcc agtgtttatg ataaatactt taaaataact gctaaaaaaa
    33901 tgagtaaaaa tatatatgat tactatccta ataaggttat gaagataagg atttttagag
    33961 ataataatta tgaagagata gagaatttat caaagcaaat aatatcaatt ttattaaata
    34021 agtctataga taaaggaaaa gtagaaaaat tgcagataaa aatggataac ttgataatgg
    34081 attctttagg tatataagca gttgtaagta ttaaaaaatt ataatctttt aattgtttat
    34141 ttaaatttag aaataaaaag ttagccatat aaaaaatatt aaaaatatat acttttcaat
    34201 atttttttga taaaataaga atgaatttgt gcaattttat ttttctccaa taactaaaaa
    34261 tagttttttg aattaatatt tgcttaagaa taaaaatcag aaacgttttt gatatagtag
    34321 gtgataatat gaaaggatta gttttagaag gtggaggaac aaaaggagcc taccaaatag
    34381 gtgcatataa agctttaaga gagcttggta tagagtttca aggagtaact ggcacatcaa
    34441 ttggtgcttt aaatggagca tatatagttc aaaatgatat tgaagtaatg gaagatattt
    34501 ggttaaatta tgactatact cattttatga atattgatag agaaacctat gagaggttta
    34561 aaaatgtaga tttcactact aaaaatctca atgcagtgtt tgagcttata aataaagcta
    34621 gaaaaaatga aggtatagat ataagcccat taagacaact aatggagaca acattaaatg
    34681 aagacataat aaggaaatca aaaaaagact ttggacttgt tacggtctgg tgggataaaa
    34741 aaattaatcc atatccattg tatatcgaag atatacctaa aggaagattg attgattatt
    34801 taattgctag ttccagtttg cctatttttc aaacgaacat gatggatgat aagttgtatc
    34861 ttgatggaat gttttgtgat aacatgccaa taggactctt ggaagacaaa ggttatgaag
    34921 atgttgttgt aattaggttg attgatgatt ttattgggaa aatgaatcta aatcgacatc
    34981 aaaatataaa tataaaaact ataataccat cagaatatct aggaggttct cttaataaag
    35041 atagagataa tgtagaaaaa aacatccgac taggttactt agatactatg aaagcatatg
    35101 aaagatgcac tggaattaag tattattttg atgtagagta taaatataat gaagaatatt
    35161 gttttgataa atttagaagt atgagtaatg aaacaataca atatatctgt aatttattga
    35221 atataaaaag agatgcttca cttagaaccg ttatggaaag tgtaatacca aagcttggtg
    35281 aggtgcttgg attgccaaag gatttttcat ataaagattt gttttactgt atatatgaaa
    35341 agaaacttga agaaaataat ataaacagga taaaattata tgattttaac aaggttatag
    35401 atgttgtaaa tacaaatatt gattctaata gaaatttagc ttctaatttt gaagttagac
    35461 ctaaaattag tattaaaaag aacaaaaaca caaaattatt gacaaattgt attataaaag
    35521 acataaaaaa tcaaagttaa ataaaaaaca aaaaaaaatt tgtaattata ctcgaaaaaa
    35581 gtgtatatta gtagtataat atttttgtag cactagtggg aacaaaaaat gaccatagga
    35641 caaaaaagta ccacttagta ccattaaagt aaaaaacagg aaaacaattt taaaaaatat
    35701 aaaatgtaaa gtttatagga ggatttaaaa aaatggaaaa agtagttagt atcaaaaatg
    35761 caagtggatt acatgctaga ccagcaggaa tgtttgttaa gaaggcagct gaatttaaat
    35821 caacagtaga ggttgtagca aaagacaaaa ctgtaaatgc aaaatcaata atgggaataa
    35881 tgagtttagg tttagctcaa ggagaagaat taaagttagt tgtaaatgga gaagatgaag
    35941 aagtagcttt agctgcaatg gttgaattag ttgaaagtgg atttggagaa tagatataat
    36001 aattttttaa aaatttgata tttagaatct aatattactt aaagtaaagt aacagagaag
    36061 tattaaacat atacagacca acatttgtag atatttaatt tctttgttac tttcatgttt
    36121 atttaatcag aagattaaga ctattgttaa ttgggtataa aaatataaat attttagaaa
    36181 atgaaataag atatttttaa tcataaattc agagtaaaaa tacagtaaca aatagtttat
    36241 aaaattttat aaaaaattat aagtaaataa aactataaaa atgtaaatta tttatataaa
    36301 tttataaagt ttatgtgcta gatgatattt tttaggaggt agggttatgg cttacaaagg
    36361 aataggtgct tctcctggtg ttgctttagg gaaagcgtta gtagttgaac atagtgaatt
    36421 agtaattgaa aaaaaatcta tagataatgt tgaagctgaa attgctaaat tagaagatgc
    36481 agttgcagtt tctaaagaag aattagtaaa agttaaggaa aaggcttctg aagaattagg
    36541 agcagaggaa gcagaaatat ttgaagcaca tttattagtt ttagaagacc cagagttaat
    36601 tggttctgca atagataaaa ttaaaactga aagtgtaaat gcagagtatg ctttaaatga
    36661 aataaaagag atgtttgtta gcatgtttga gtctatggac aatgagtaca tgaaagaaag
    36721 agcggcagat ataaaagatg ttacaaatag aatacttaga catatattag gaattaaagt
    36781 tgttgattta tctgctttaa gcgaagaagt tgtattaata gcacatgatt taactccatc
    36841 agacacagca actatgaata aaaaaatggt tttaggattt ttaactgata taggtggaag
    36901 aacttctcat actgctatca tgtctagaac tttagaaata gctgcaatcg ttggattaaa
    36961 tgatataact tcaaaagtta aggatggaga ttttgtggtt ttcaatggag acacaggtga
    37021 agtaatcgtg aatcctgatg aagagactat aaataaatat actgaattaa aagctaagta
    37081 tgaagatgag agaaaagcat tacagctatt aaaaggaaaa ccatctgtaa ctttagatgg
    37141 aaaacatgtg gaattagctg gtaacatagg aacacctaac gatatagaag gtctaataaa
    37201 gaatgatgct gaaggtgttg gtctttatag aacagaattt ttatacatgg atagagattc
    37261 tttcccaaca gaagaaatac aatatgaagc atacaaagct gtattagaag gaatggatgg
    37321 aaagcctata gttataagaa cattggatat aggtggagat aaagaattaa gttatttaag
    37381 tatggaacct gaaatgaatc cattcttagg ttacagagca ataagacttt gcctagatag
    37441 aaaagatata tttaagactc aattgagagc tttatataga gctagtgttc atggaagatt
    37501 aagaatcatg ttcccaatga tttcatcttt agaagagttg ttacaagcta aagaagttgt
    37561 aaaagaagtt ttagcagaac ttgattctga aggtgtggct tatgctaaag atgtagaaat
    37621 tggtatgatg atagaagttc catctgctgc tgtgatatct gatgtattgg ctaaacacgt
    37681 agatttcttc tctataggaa ctaatgattt aattcaatac acttgtgcag ttgatagaat
    37741 gaatcaaaag ataagttatc tttacaatca atttaatcca gctgttttaa gacttataaa
    37801 gactgttata gacaatgcac ataaagaagg aaaatgggca ggtatgtgtg gagaatctgc
    37861 tggagaccaa aaaatgatac caatactttt aggtatggga ttagacgagt tctctatgtc
    37921 tccaatatca atacttcctg ctagaaagtt aataacttca gttaaagagt cagatatgaa
    37981 aaaattagct gatgatgtat ttaatatggg tactgctgaa gaaataaaaa gctatataga
    38041 aaagactttt aatatataat taatgttata tagttactaa aattcatttt tctaaataac
    38101 aaaattatat tttacgaata aaaaaactat agtatatcat atatactata gtttttttgt
    38161 tatataaatt acgtatcaaa atttgtaaaa tgaatagata taattactaa atatgtgata
    38221 gtattggcta taataggata taaaaaataa tattgtaata aaaagtttat atttagtaat
    38281 gaaaatttaa cgttttactt gaaattatgt gttttgtaat gtattattaa ggttctaata
    38341 ggaacatttt catcaatttt tctatagtgg gggtaggaat tatgaggtac aaaaaaagaa
    38401 atatagataa gtcaagtgaa ctttcatttc aatctctagc aaaatatttt ataactttct
    38461 atatagtaat tatattaatt attctttcta tgttttgtat aaatacaata tgggttatga
    38521 aaaaagataa tcaagattta ttatctacaa cgtcaaatcg tattgaagat agggtaggag
    38581 aaacaattaa gctattgaac tctttgagta aagataatta cataatagat aatagtgtga
    38641 atggcataga taaaagtaaa gaactttcaa tgtatagtga tgaatttggg tatatgatga
    38701 tatgttttgt tgataagaat ataattgtat gggatgaaga aggtggtaat attagtctag
    38761 ctggtcgtga ttatatgcag aaactttttg caacaaaaaa atatcaagtt acagatagtt
    38821 ttgcagcagg ggctgatgga aaaactttaa actacactat tgcatcacct ataataaaga
    38881 atggaaatgt agatggttgt gtatttgcag caatttactt tgatgagata aaggatatac
    38941 tcaaacaagg aaaaggtcaa cagcaacaga atttcatatt atttggaagt gaaaatcaaa
    39001 taatgaattt aacaagtgat gaaactgcat atggagagaa gtatatgaag ttatcgcaga
    39061 aaacaaaata tcttggaacc aatgcaaaaa aagtagaaaa ggatatgttt aataataaat
    39121 caggtgatta ttgggctttt gaaaatggaa aattatactt tatgtcctat aaaccaatta
    39181 agaacacaaa ttggagttta ttttgtaaag ttaatttctt agatattttt gcattaaatt
    39241 taattcctat gggagctatt ctatctgtta taactttagt atttattatt ttgatttgta
    39301 tggagaaaag cttcatgaaa aaacgaatgg aagttattga tatgctttta ggttctgttc
    39361 aagaattaga aaaacgtata tatcaaacag aaagacctag tgaagtagac tttaaagaaa
    39421 ttattcagct cacaagtaaa ggtctgactg atggattaac tggtgttgta acacgtactg
    39481 tattttttga ccaaatttat aatcgtgttg aaatggagga aaatgataaa ttatctgtaa
    39541 tttcatttgt tgatttagat gatttgaaat gtattaacga cttatatgga catgaaattg
    39601 gggatatggt tttgaaaaat gttggatatg tacttcgtga atatgagata aaatataatg
    39661 gtttaattgg gcgctatggt ggagatgaat ttgttatgtt gttatctggt tttacatcga
    39721 gagaagaggt ggaggattta ttggatgaga ttgctattcg cttacatact gaagtactaa
    39781 cagatgatgg tatcattcaa actcattgta gtgttggagc tgtagtatgt agtgtaaaag
    39841 acaataaaga tatagataat ttattaaaac aggctgatga agctttatac tatgttaaac
    39901 gtaatggaaa aggtaattat catttagttt aatgtatgtg gaaggagttt atatcatatg
    39961 aaaaatgtat ctaaaaaatt ttttgtatgt actattattg tattaataac atttttatgt
    40021 tttagtaaat ttgctattta tgtttttgac aaacaagaaa agaaagaaac aaaagtagta
    40081 gaaaacatgt taaatgtcta caagaataac attattttaa atctgactga aaaatttaat
    40141 tatagcaaag acttgacatc agttttagtt tcaaacaatt attctataga taaattaaaa
    40201 cctattgcag atgaaattct taaaaacaat tcttgcatat atatttcagt tgtagaaaaa
    40261 tataatgtag ctgcattttt ttctaattct gtcaaaaaag atattatcaa tactccaata
    40321 caggaactga attattctta ttcattagca aattctatca aaggattagt cgtagaaggt
    40381 ccttctaaag accagttttc aaatggagat gtgtttttaa ttactaatcc tatttataaa
    40441 aatggagagt actggggaga aattacagta gctttagatg ccaaaaaagt tgtagattct
    40501 tttaacttaa ataaattaaa agaatctgga tttgagtatg aactttggcg tgtacatcta
    40561 gataatggtt caaaatctac aatagaaatt tctactaaag attttaattt ttccaaatca
    40621 catgaaatta agtttaaact tccgactgtt tggactctta gcattgtgta caaaaatgga
    40681 tggaataata agaatataga ttttattgta tttgttatat tgttaattat ttccatatta
    40741 ataggtgtga tgacatttat ttacattgaa aagaagtcga ttagtaagga attaaatcaa
    40801 cagagtctta aaaatttaga aactggtatt ttgaatagag aaggtttttt aattaagtta
    40861 caagagcatc tgaatagaaa aaataatgaa atagtgttgc tgtatatatg tattactaat
    40921 tatagtaaat ttgctccagt tatgagcttt gaaaaaagac aagattatct tacacatata
    40981 gtaaaaaatc tagataatta cataaaaagt tctcatttta ttgctcgtat aagtgaggaa
    41041 ggttttgttc tagctttaga agaaaattgt gataaaaatg aattgcttaa cattgaaaaa
    41101 gggatttcct tagagctttt acgaaaaatt aaagtagaaa ataaacaagt atttttaacg
    41161 attggttatg gctcatctag gtgtgttgat gatagaaaag atgcagaatc tctgttactt
    41221 tcagctattg aagattatga gagaagattt ccagcaatgt ctcctattca tgattttact
    41281 aaaaagtgtg aagatatgct tgatgaaaag aaacagattg tatttggtga atatccagat
    41341 ttagaaataa acaagttgtc taaagtaata tacaaatatt ataaaaaagt acagcgtatt
    41401 atgtataaag attataatat tgatattgga aatagacgag ggtatatgcg tgatgtcgaa
    41461 gtattaattg attataattc tgagagaatg ttatacttat ttttaataga catatgcaag
    41521 tttggaaaat ttaatgaact gttttctatt gaaataggag atgttatttt aaaagaagtt
    41581 agtaacaaat taaaaacaat tttttctaat tatctatatc gtattaatgg agatgtattt
    41641 ttaggcattt caatagataa agaatcttac ttagatactt tacataaggt taatgaagta
    41701 tttaatagtt ctttaagtgt agaagatgta ctttttgata taaaatgtaa tattggtatt
    41761 tgtcaatatc catatcatgg tgaaaatcca tatgaccttc tggaaaaggt acaaattgca
    41821 ttggagtatg caaaaaagga tgtcaaaagt aatgttaaag tttatgataa ggaattgaca
    41881 gaagagattt accatgaaaa taatattatt aaggatttaa aaaaaggtct ggaagaacaa
    41941 acactagaag tttggtatca gccaattttt aatgttagta aaaactgtta tataaaagct
    42001 gaggcactaa ttagattgag aaataaagag ggcggttatt attctgccta tgaagttatt
    42061 acttatgcag aaaagcatgg acttattaag gatgtaggtc tatatgtact aaaaaaggct
    42121 tgtgatgtaa ctacaaaaat aaaagataaa aatatagatt ttgaacaaat gcaattaaat
    42181 ttatctgttc agcagattgt acataagcag tgtgcaaatg acatattatc atgtattgag
    42241 aattgtggtc tagataaaaa gtatatttgt gttgaagtta cagaaacagt acttatggaa
    42301 acttttgaaa ttgtaaatgt tactcttgat aaactaaaaa aagaaggcat tagcatagct
    42361 ttagatgagt ttggttctgg atttagtagt ataaattatc tggcaaattt acctattgaa
    42421 aatttaaaaa tagataagga attagtagaa caaattacct attctaaaaa acaatttgaa
    42481 ttattaaaaa ctatagttca aattgctaag attaatggca tggatattgt agcagaaggt
    42541 gttgaaacaa aagaaactct ggatttagtt gtaacttcag gtgtgaattt gattcaaggt
    42601 cattattttt cagaaccttt aagtgaagat aaatttattg aacttatgat tgaagaataa
    42661 aggttaataa aataaagatt taaggtattg aaaaataatt aaaagtattt aataatagaa
    42721 ttagggaaga ataagtttat aagtaaaaat aattgattag tagttaataa aataaatgtg
    42781 atatccaaat aaatcctaaa tctaatttga gttttaatat agatagtcta aagtttgatt
    42841 attaaagatt taattgtaag ttgttacagt tgatgatatt gattagagat taaagtttag
    42901 agtagaaagt taccttaaat ttaaaaatgc aagataaccc aatctaaaaa ttataatcac
    42961 tcgataggtt taaggattta tttgtttatt ataaatagga gggtgtctca aaatgaactt
    43021 tttagtttat gaggcactct cttttatata gaattaaata tattttatta tttcaacaat
    43081 gaaaaagaag cttatctcta ttttgagaca gcctctttgt atactattat taatttaatc
    43141 aataacataa tttaaaatta tattattagt taagatttcc tgctttatta gtaaataatt
    43201 ctttaatagc attgtttctg gaaactaaat cgttatcaga gttgtttgtt tttttcataa
    43261 tcactgaata acgtttttta tttataaaaa atattaaaat cttatcatca tcaaaattgt
    43321 attctttata tctgtcaata gactcataac ctagatttag aatgtttttg tcttttatac
    43381 tatctacatc taaaatatat gatgggtcag cctttagtaa tatattgatt aaacattttt
    43441 cttcaccagc agagaataac tccatcttac ttgatataga tttagattgc tctgaagtta
    43501 tttttgcagg aataaggttg tcattatcat ctcttagaag ataaccatgt gtgtcttgtt
    43561 gatatactaa attaaccttt ttcttaggaa cactaatcat tttatctaaa tgtacataat
    43621 gatgattttc aacaaaacct tcatttaata tttcaacaaa gttattaatt ttacctaggc
    43681 aaaaagattc tatagattct ttacagaatt tatttgtaaa tggtattatg tttacgtttt
    43741 cctcgtctgt attccatgct atagcataat ccaacgaata tacttcttcc tctatatctg
    43801 caaaattata ttgtactatg tttcctttca aaattactcg acctccatct tataatatat
    43861 ttaatatact ttataaaact tatattatat ttaaataata tcataaattg gataaatatg
    43921 atatagtaaa taagaaaaaa tttgaaaaaa atcactataa atgagtaata aatataaata
    43981 gtgtatacta atgggatgga aaatgtccac tattaacttt attaccccac atatataaga
    44041 taaaattatt aatataaaat ataatctatc gacaatgaat agacttttaa ataaacttaa
    44101 tataaaatta attaaataag tgcttaaaaa cgatttactt taatgataga atcatattaa
    44161 aggaggataa ttttatggat ataaagaata aaattaaatc tatattttta ccagaaaaaa
    44221 atgactattt agatgatgaa gaatttgagt atgatataga aaataatgaa gaagatttaa
    44281 aggaagataa caaagtacat ttaaatgatt taagtagagt aaaatatgat tacagagagt
    44341 taaaagacat agaaaaccaa acagaagaga tatgtctgct agcagtaaag caggatggta
    44401 ctataattga gtttgtaaag gacaagactt ataaagtatg tatggaagca gtaaaacaga
    44461 catataaatc tcttaagtat ataacaaatc aaaatgagga tatttgtata gaagcagtaa
    44521 aacagaacta cagagctttg tactacataa ataataagac agaaaatgta ctaattgagg
    44581 ctataaaaaa tgcatcaact tatgatgtca tggaagtatt taaatttgtg gaagagcaaa
    44641 cagaagatgt atgtctagca tttatagaaa gggcatctaa aaatgatgtt gcagagatat
    44701 taaaaggaat aaaagagcaa actccagcca tatgtttaga agctgtaaaa aaagatggaa
    44761 aatctttagc ttatgtaaag gaacaaagta atagtatatg tttagaagct gtaaaagaaa
    44821 actacagtgc actatcttgt gtaaaagagc aaactgaaga aatatgtata gaagcagtaa
    44881 aacagaatga ctttgcattg tattatgtaa atgaacagac agaaaaaata tgtatggaag
    44941 cagtaaaaag aagttatatg gctctacagt atgtaaataa gcaaacagaa gagatttgtt
    45001 tggaagcagt aagaattgat ggaagagcat tacagtacgt aaaagagcaa acagaagaaa
    45061 tttgcttgga gtcagttaga caaaatggaa aagtactgca atatgtaaaa aaacagactg
    45121 aaaatatatg tatagaagca gtaagaggtt catttgaaga acttgaaata aaagaaatat
    45181 taagttatgt aaagatacca acagaaagaa tatttgtaga agcagtaaag caaaatggaa
    45241 aaattcttaa atatgtagaa aatcagacag aattaatatg tttagaggca gtaagagaaa
    45301 actataatgc tttagcttat gtaaaggaac aaacagaaaa gatttgttta gaagcagtta
    45361 atcaaagtta tgaagcgctt aaatatgtga aagaacaaac agaagaagtt tgtctaaaag
    45421 cagtaaaaca agattataga atgttgaaat atgtaaataa tcaaacagaa aaaatatgtt
    45481 tagaagcagt aaagcaaaat tatagagctt tagaatttgt tgataatcaa acagaaaaag
    45541 tttgtcttga agcagtaaaa cagaatagaa aagcactaca gtatgtaaaa caaaaacaaa
    45601 gttaaaaggc taaaatatat agaaaatata tgcaaataaa taaaatttga tattttgtaa
    45661 taaataaaat gggtatctca aaaggaactt tttagtttgt gaggtactct ttttatatga
    45721 aataaaatat atttttagac ttttaacaat ggggaaaagg tttatttcta ttttgacata
    45781 ggctttttat gcatataaag cttgataaaa ttgttttatt ataattgttt tgttatataa
    45841 ataataagaa gaaaattata taggtctttt tttgaccagt tttacatttt taacaactag
    45901 ttttgtaaag tatattgaat tttctcaaaa aagaatgtac tctatatatg taaaattttt
    45961 ttggaggtaa aaatgtttaa aagatatgct gaaaaaatga catctgtttt aatttgtaat
    46021 aatatgatag ataataatga atctaaagtc tattcttatg gatttgaaat attaatagct
    46081 ttcatagtta atataaccac catgctgttt ataggttttt tatttggtaa atttacatat
    46141 gtattatttt ttttaatgtg ttattgtcca ataagacaat tttcaggagg gtatcatgca
    46201 gacaattatt ttagatgctt attaaccttt atttttataa ttttatccac aatcttaatt
    46261 atagaaaata taaatataga cttatttaaa aatattatta tgattattgc aagtgttagt
    46321 tgggttggaa tttgtgttct gtgcccaata gaacatagga gtaaccctat aagtgatagg
    46381 gaaaagttgg tttataagaa aactgctata tttatatcta cagtagtgtt attgataaca
    46441 attttaagtc tttctttgag tatatttgta gattatttca catatagcgc atttgccatg
    46501 ttttggattt ttgtgatgtt agtattaggt aagttaaaag ctaaagtata agtaaaatat
    46561 gtatgaggga gagttaagtt atgaaaaagt ttattgtaag atttatgaag tttgctagct
    46621 cattggcact tagcactgct attttgtcag ctaatagtac ctgtccttgg attattcatc
    46681 agcctaaagt acccaaagaa atcagcaatc taaagaagac aaattaaata caagcactta
    46741 tttatacaaa attatcagga aaaaagaaat tattatattt taataagatt aaaacaatat
    46801 aaaatcagat acaaaattta ttttatttat tttataggag cctaatacct atttggtaat
    46861 atattagtgc gccaatttag ggggatatag tgtatttaat gaaatgactt acggagagta
    46921 agtcattttt gtattgtatg tattttatgt aaacttatga tataatgaag ggtaaagttt
    46981 aattaaaata ataaaaaact ttagtataga tagtaattta ggaggtagag tatgagtatg
    47041 atacataagt tttctatgaa tggatataat atcgttttag atgtgaatgg tggagctgtt
    47101 catgtgttag atgatgttgc atacgatttg ttagattttt ataaagaaaa aagtaaagaa
    47161 gaaatattag agatattaaa atcaaaatat caagaagaaa aaataaatga agcatatgaa
    47221 gaaatattaa atttggaaaa agagggtctt ttatatacag aagatacata tcaatatcat
    47281 ccaagctttg tacatagaga gcctgtagta aaagctttat gtttaaatgt agctcatgat
    47341 tgtaacttaa aatgtaaata ctgttttgca gctcaaggtg attttggagg agaaaaagaa
    47401 cttatgagtt ttgaagttgg taaggcagca attgactatt tgattgctaa ttcaggaagt
    47461 agaaagaacc tagagataga tttctttggt ggagagcctt tgatgaactt tgaagttgta
    47521 aaacaactgg ttgactatgg aagaagtgta gaaaaagatt ataataaaaa tataagattt
    47581 acaataacta caaatggtgt actgttaaat gatgagatta tagattatat aaatgaaaat
    47641 atgcataatg tagttttaag tttagatgga agaaaagaag ttaatgataa tatgagacca
    47701 actttaaatg ataaaggaag ttatgatatt acattaccac gatttaagaa acttgtggaa
    47761 aaaagagcaa aagataagta ttattatata agaggtactt ttacaaggga taatttagat
    47821 ttttcaaaag atgttatgca ttttgcagat ttaggattta aattaacctc tgtagagcca
    47881 gtcgttggag atgagagtaa tccatatgca cttagagaag aagatttacc taagatattt
    47941 gaagagtacg aaaaatttgc tgtagagtat gcagatagac aattacaagg agatggattt
    48001 aaatttttcc attttatgat agacctaaat caaggtccat gtgtaataaa gagaataact
    48061 ggatgtggag ctggaaatga gtatctttct gtaactccaa atggagatat ctatccatgt
    48121 catcaatttg ttggaaatga agaatttaaa atggcaaata tatttgatga agaaatagta
    48181 cttccagaaa atttaaaaaa tatgtttaga gaagctcatg tatatactaa agaagagtgt
    48241 aaacaatgtt ggaataagtt ttactgttca ggtggatgtc atgcaaatgc gataaacttt
    48301 aataatgaca tatcaaaacc atatgaatta ggctgtgaaa tgcaaagaaa acgtacagag
    48361 tgctctataa tgatacaagc taagttaatg ttagaaggag caaccaacta aatttatatt
    48421 ttaaaggagt tgtttatata tgattagaga ttatttagaa gataaacctt taattgacga
    48481 aagtgtattt gtggcaaaat cagcagatgt aattggaaat gttaaaatag gcaaagattc
    48541 aagtatatgg tacaatgcag tagttagggg tgatgaagga ccaataacta ttggagaaaa
    48601 cactaatata caagattgtt ctatagtgca tggagataca gaaactataa ttggtaacaa
    48661 tgtaacagtt gggcataggt ctatagttca tggttgtaaa attagtgata atgtgttaat
    48721 tggtatgggt tcaataatat tggataatgc tgaaatagga gaatatactc tgataggagc
    48781 aggtacacta attacatcta ataagaagtt tccaccaggt gttttgataa tgggttctcc
    48841 tggtaaggtt gttagagagc ttacagaaga agataaaaaa tatattgatg aatcttatga
    48901 atggtattta gaggcagcac aaaatcaaaa atattagttt aaagagtggt tgtttcaaaa
    48961 catatttaaa tctgttttga ggcaaccact aatttatttt attagatttt gttgata
//
LOCUS       DAGSRM010000023        48317 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.023, whole genome shotgun sequence.
ACCESSION   DAGSRM010000023 DAGSRM010000000
VERSION     DAGSRM010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 48317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48317
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.023"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            172..1485
                     /locus_tag="MJE33_001403"
     CDS             172..1485
                     /locus_tag="MJE33_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBY9647344.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMANVLPIAVGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIVFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITVGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKTLLKVNSGGSPVNSI
                     LLITVLACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQKPDLHRPYKVPLYP
                     IIPIIAILGGLFVIINQILTSTVISLGGIFITLLGLPVYYYMKKR"
     gene            1657..2832
                     /locus_tag="MJE33_001404"
     CDS             1657..2832
                     /locus_tag="MJE33_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBY9647345.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDSKKRYIEDLKIEMELY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFISIKEMFDIDEN
                     AEITIECNPGTLTLEKLKTMKEIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINIDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENVDIEMYEYTINYLKSKGYNQYEISNYSKEGYNCEHNILYWECEHYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFREKYNKQIEMLLARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            2935..3954
                     /gene="hrcA"
                     /locus_tag="MJE33_001405"
     CDS             2935..3954
                     /gene="hrcA"
                     /locus_tag="MJE33_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HBY9647346.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLINQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            3984..4604
                     /gene="grpE"
                     /locus_tag="MJE33_001406"
     CDS             3984..4604
                     /gene="grpE"
                     /locus_tag="MJE33_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBY9647347.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSSEKT
                     DEKEVDDENVTDINSKLAEKKLQDELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACEDKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESVDGVEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            4696..6543
                     /gene="dnaK"
                     /locus_tag="MJE33_001407"
     CDS             4696..6543
                     /gene="dnaK"
                     /locus_tag="MJE33_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBY9647348.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLAEEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSEAEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSGEKEDIEKAIADLKAVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            6754..7908
                     /gene="dnaJ"
                     /locus_tag="MJE33_001408"
     CDS             6754..7908
                     /gene="dnaJ"
                     /locus_tag="MJE33_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBY9647349.1"
                     /translation="MSTKRDYYEVLGISKGAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGGFGGQGFGGFEDMFGDI
                     FGDMFGGSFGGGRARRRGPQRGADIRQSVTISFEEAAFGKKMSIKVNRSEECEECNGT
                     GAKPGTSKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSACNGTGEVIESPCSKCHGTG
                     NTRKVKTIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGND
                     IYFEMPITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGD
                     QYVKVVVEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
     gene            8874..9809
                     /locus_tag="MJE33_001409"
     CDS             8874..9809
                     /locus_tag="MJE33_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBY9647350.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRSECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKRRFPD
                     VEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKEYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EDLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            9845..11218
                     /locus_tag="MJE33_001410"
     CDS             9845..11218
                     /locus_tag="MJE33_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY9647351.1"
                     /translation="MGKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLASAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLIFGIILMCLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGKWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEVNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
     gene            complement(11406..12173)
                     /locus_tag="MJE33_001411"
     CDS             complement(11406..12173)
                     /locus_tag="MJE33_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY9647352.1"
                     /translation="MEELKDFLERQLNKKINIYPYENQKLDASSHLVTFNNAIYVIDF
                     LDKNNLDNLKGNFYLIYQSHIDFEYLKNIFYNLFEDINIIQHNGFFIVNSKYNLDINV
                     TTQNIIETETYQSTYIFYLGKLDSKADFDFRLQLCSDLLPHIIKDNAENKFLNLFDLI
                     RYKTLDLINEDNILNKLIDFNKIKSIDEELLYTGIKFINNDLNISKTSTSMFLHRNTL
                     VYRLEKINEILGFDLKNFENAMIFYLSVKSYFLYKKI"
     gene            complement(12203..13312)
                     /gene="ugpC"
                     /locus_tag="MJE33_001412"
     CDS             complement(12203..13312)
                     /gene="ugpC"
                     /locus_tag="MJE33_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBY9647353.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNDIEPKDRDIAMVFQNYALYPHLSVYENMAFALK
                     LRKLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGVVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVILIEENDEIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRVHIFDKETTLAIR"
     gene            13570..14307
                     /gene="cas6"
                     /locus_tag="MJE33_001413"
     CDS             13570..14307
                     /gene="cas6"
                     /locus_tag="MJE33_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBY9647354.1"
                     /translation="MSRISIFMESCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFN
                     YGKERKNNKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKV
                     KELEYKGYRFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLN
                     YIANLTLESIRGSGLRKPLEFIPLNFKKRVLKEKIRGFKVREFYYINAYAGTFFLKGD
                     MEDLNALYKMGIGYRRTENAGMVDILK"
     gene            14312..15787
                     /locus_tag="MJE33_001414"
     CDS             14312..15787
                     /locus_tag="MJE33_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647355.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFYELLMDLKNELEKNDYKLKFT
                     CELNRNYLSLESAEYIDPKYISDYILENQLFKVFKNGKKETLEKIFSNIDNLTYVNFI
                     EELDKSSATAKQKEDIKKDFKNRRFPYVRNSGKYGLNTSLENMETNVKRIVDTVIKSN
                     IEIDEIDKLDLTQYEEKDSVCNVCNINKTTKLDIDLRERKDSKYNFLFRGAEKSGFKR
                     SGQVESNICFECEFFNLMCLLYINLKRPMVFAYTNDLRELAFLNHKIMLKRKMYSDKS
                     FYKKLLHEKISSIRLYRFDIVKNKGIILKFDSIIEYKELLKKIELIDIIDNYNFSREP
                     VNTKNLGKKMIDNGNLSNLKELLLDNISFLKQSTGTSREMDFVSSSYNIGLYIKLCWI
                     VDGGEEYKMKNYMESNLIYSRVGKDLFNKMTDESRRKNFSMKVIQLLKSNDRTQLFQT
                     IMHVLVSNGILIGEGFVEGLMQSSELELNTNVGLFIQEIMK"
     gene            15807..16820
                     /locus_tag="MJE33_001415"
     CDS             15807..16820
                     /locus_tag="MJE33_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DevR family CRISPR-associated autoregulator"
                     /protein_id="HBY9647356.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYREKIHA
                     KTSIATITYDLRRMLHQEYGWNLSNIVFGKKGNIYPSIKKNVGCVDENGLETDVFGYL
                     IPLKDKGSISKASPLRIIPFRSLNPYIGSTQLITNRGFLSSEFGRKYYDEKEENEVPR
                     DENFPTTQALAIEETLSDYYVYTITLELDRIGVVEVEDGKLLLPEERKFMSKELREKA
                     VKDILDAITVFTRNIKHSSVLLKPLAVIGGAFDKVVPFFWDDVDYNADSGEINLEGVI
                     ETIESYSLKESNTILAINDRLKISNKKELNKFNLSKYPVKEIKNLADRLEIGEDNMWY
                     LKE"
     gene            16836..17633
                     /gene="cas5"
                     /locus_tag="MJE33_001416"
     CDS             16836..17633
                     /gene="cas5"
                     /locus_tag="MJE33_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HBY9647357.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLNSDIDIGIIGTHHHRELEFSRGENIDFWNEYTNMRKGKDKEKFLLQGNYYDYY
                     KEHKAQNSILNYEVLKEVELTIFISCKDDEELEFIKKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVELKEVIPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPH
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCIDTKEVFTWL"
     gene            17660..20068
                     /locus_tag="MJE33_001417"
     CDS             17660..20068
                     /locus_tag="MJE33_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBY9647358.1"
                     /translation="MKNIMQKEILAKSYNLRTNQQEQTLVEHIKDLFEVLESILELNL
                     YSDKDVEILKICCALHDLGKINSIMQQKMEINNKISYSCSEEERKKLESDKKSLKKIA
                     RHNIFSGAFLKDILEKMNLSEEDKLYIYKSIMLHHGNYEDYMRLSISKVQEEIYEYIE
                     KGILEKEEFNLKDIESYINNILNVDFKFGEDVLDYDYIDKLSESMFIDSDYNNGQIDD
                     SVLNYRKFKYILYKGMLNLIDHSASSRQKGIKFYNDFTDEEIDNMILNEIYKSQGNLE
                     KNNIEFNTIQKRLRELSGRNVLTVAFTGSGKTAADYRKTFKRKFFLVPNKISAESFYR
                     KNIFQNKNDLDTRSSNDYIGLLHGDINLYSENEDNGEHDFVLTLRDIDLSINFCKPCV
                     IATVDQLLLSMFKFPGYEKIFAAVKNASITVDEVHLLEPKMFLILIYFMQFACKYLDV
                     DFHLMTATLPKSYKEQMINKGIIFQEESNKNVTDKGEIVFIESNKEEEICEGKDVKVS
                     FIKEKEIKSIVEDALENKQKILIIKNTIDSVNRTYEFLKENLSDKYGGVDIDVLHSRF
                     KFKDKKEKYSKILNGEGDIWISTQSVEISLDLDFNIIISDLATMDSLIQRMGRCNRNN
                     KYEYGNFYILPSEDKIYDNKLKNTTKNILKDILKTNSIFTMGIRKTILDDYYDNSVVK
                     KYFEENFISCDKEIKNIYGINKEIFDGLDLIFNFEPYKNIVDSKKEAAKIFRDIDVSY
                     KIILEEDFYKEDRELQQDSIQVSGFIFNRLYYYGLISKVEGYMVLKSSSKFEYNSTVG
                     LILK"
     gene            20509..21456
                     /gene="prmA"
                     /locus_tag="MJE33_001418"
     CDS             20509..21456
                     /gene="prmA"
                     /locus_tag="MJE33_001418"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBY9647359.1"
                     /translation="MNNWIEVTIKTTTEAVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKDFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQEGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKK"
     gene            21475..22233
                     /locus_tag="MJE33_001419"
     CDS             21475..22233
                     /locus_tag="MJE33_001419"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBY9647360.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRCKLGEKLEICD
                     KNNNEYICEIMNIDKSIVNLEILEKVDINRESELKVRLYQGLPKAPKMEMILQKLTEV
                     GVEEIILVQTKRSVVKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLSFKEAL
                     EDMKKNNVNICPYENERTVSIKHALKKCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            22235..23533
                     /gene="mtaB"
                     /locus_tag="MJE33_001420"
     CDS             22235..23533
                     /gene="mtaB"
                     /locus_tag="MJE33_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HBY9647361.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKKINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INQIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLKEIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRELDVLFEQNIEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            23576..23926
                     /locus_tag="MJE33_001421"
     CDS             23576..23926
                     /locus_tag="MJE33_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HBY9647362.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDIPDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            24089..24268
                     /locus_tag="MJE33_001422"
     CDS             24089..24268
                     /locus_tag="MJE33_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBY9647363.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            24298..24741
                     /locus_tag="MJE33_001423"
     CDS             24298..24741
                     /locus_tag="MJE33_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBY9647364.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            24852..25349
                     /locus_tag="MJE33_001424"
     CDS             24852..25349
                     /locus_tag="MJE33_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBY9647365.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLIYLMWLVYAGFLNYFI
                     WFINK"
     gene            25542..26105
                     /gene="raiA"
                     /locus_tag="MJE33_001425"
     CDS             25542..26105
                     /gene="raiA"
                     /locus_tag="MJE33_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBY9647366.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENLDFIDNSSEFDDYDYDDEEDENIVIERRKKFNVKPMSSEEAILQMELVGHNFY
                     MFRNQDNFEINIVYKRKAGGYGLIEQD"
     gene            26318..26554
                     /locus_tag="MJE33_001426"
     CDS             26318..26554
                     /locus_tag="MJE33_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HBY9647367.1"
                     /translation="MLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIKTK
                     VKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            26567..27910
                     /gene="yqfD"
                     /locus_tag="MJE33_001427"
     CDS             26567..27910
                     /gene="yqfD"
                     /locus_tag="MJE33_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HBY9647368.1"
                     /translation="MISFIRGYYVIVVEGVGLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKDFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRNELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKRNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEVPSNPEKRPLNDIRNEFDEDNKDNKDQNNSDENNSNQNSNNSQNNNSNNN"
     gene            27849..28964
                     /locus_tag="MJE33_001428"
     CDS             27849..28964
                     /locus_tag="MJE33_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBY9647369.1"
                     /translation="MKIIVIKIVIIVKIIIAITTNKIKLKILGGLIQLIIQKKFTIAD
                     GNFERELFGNFDENVKLIEKTLNIDVILREGNIILIGEEKNVDSALKLMNELHQTVSN
                     GKHLDKQSISYSLSLLLEGSEQKIKELEGTIVITQRGKAVQPKTLGQKEYIKLIENND
                     ITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRIILTRPAVEAGESLGFLPGDLKDKVDP
                     YLRPLYDALFEMLGADKFNKYLERGTIEVAPLAFMRGRTLDNSFIILDEAQNTTSEQM
                     KMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLIQATEILKGVPGIGSIMLTDRDVVRH
                     ELVQRIIRAYEKHDKREEFKKEERKKAKMQEKTFKRK"
     gene            29021..29482
                     /gene="ybeY"
                     /locus_tag="MJE33_001429"
     CDS             29021..29482
                     /gene="ybeY"
                     /locus_tag="MJE33_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBY9647370.1"
                     /translation="MDLILDDRQDKLEVSEELIEKIKDIIIECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            29488..30207
                     /locus_tag="MJE33_001430"
     CDS             29488..30207
                     /locus_tag="MJE33_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBY9647371.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            30326..30727
                     /locus_tag="MJE33_001431"
     CDS             30326..30727
                     /locus_tag="MJE33_001431"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBY9647372.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSGFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIYKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHSIKDLLPNYFSGNDLK"
     gene            30791..31684
                     /gene="era"
                     /locus_tag="MJE33_001432"
     CDS             30791..31684
                     /gene="era"
                     /locus_tag="MJE33_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBY9647373.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARNDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            31696..33075
                     /gene="mgtE"
                     /locus_tag="MJE33_001433"
     CDS             31696..33075
                     /gene="mgtE"
                     /locus_tag="MJE33_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBY9647374.1"
                     /translation="MDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFDIME
                     NLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDMADK
                     LSELEEEEREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEAIDH
                     MREEAEEAETIYYIYVVDDEEKLVGVLSLRELIIARDANIVEDLMSENIISVYVDEDR
                     EEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHEREV
                     AEKENPTLREQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFIPVV
                     IGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVMRKL
                     DIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVYFII
                     TTALLSKIV"
     gene            33148..33303
                     /locus_tag="MJE33_001434"
     CDS             33148..33303
                     /locus_tag="MJE33_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HBY9647375.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            33269..34042
                     /gene="recO"
                     /locus_tag="MJE33_001435"
     CDS             33269..34042
                     /gene="recO"
                     /locus_tag="MJE33_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBY9647376.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTISLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            34035..34634
                     /locus_tag="MJE33_001436"
     CDS             34035..34634
                     /locus_tag="MJE33_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HBY9647377.1"
                     /translation="MSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYLE
                     NKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIPL
                     TVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMITN
                     AAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            34794..35672
                     /gene="glyQ"
                     /locus_tag="MJE33_001437"
     CDS             34794..35672
                     /gene="glyQ"
                     /locus_tag="MJE33_001437"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBY9647378.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            35672..37738
                     /locus_tag="MJE33_001438"
     CDS             35672..37738
                     /locus_tag="MJE33_001438"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBY9647379.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQNDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKEEYIFATIKQDGKETSEVLKDILPETIKSVVFPKAMRWGGKNLKFARPI
                     RWIVALLNDKVLEFDLEGIISSNVTKGHRFLGESTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIAGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKVLIEHALD
                     NYSELDFDKEEVVEQIMNFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIKLYNEFKQVKIKVNE
                     LLKDKKYGIALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLEICDL
                     SKIVYK"
     gene            complement(37904..39451)
                     /locus_tag="MJE33_001439"
     CDS             complement(37904..39451)
                     /locus_tag="MJE33_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HBY9647380.1"
                     /translation="MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDI
                     MEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEELFLEKFKEYYKLEKENGS
                     LELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDN
                     VENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNEEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGIST
                     DTFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASSGNFYEWVNSNDNILNK
                     ITDKYKI"
     gene            39731..40498
                     /locus_tag="MJE33_001440"
     CDS             39731..40498
                     /locus_tag="MJE33_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBY9647381.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
     gene            40781..40978
                     /locus_tag="MJE33_001441"
     CDS             40781..40978
                     /locus_tag="MJE33_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBY9647382.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            41007..42083
                     /gene="vorB"
                     /locus_tag="MJE33_001442"
     CDS             41007..42083
                     /gene="vorB"
                     /locus_tag="MJE33_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBY9647383.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            42086..42805
                     /locus_tag="MJE33_001443"
     CDS             42086..42805
                     /locus_tag="MJE33_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBY9647384.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTIEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            42818..43357
                     /locus_tag="MJE33_001444"
     CDS             42818..43357
                     /locus_tag="MJE33_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBY9647385.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIKVPVTEIAQNLGNEKAANICMLGAMVKSLDLFTKEEFESGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            43514..44572
                     /gene="buk"
                     /locus_tag="MJE33_001445"
     CDS             43514..44572
                     /gene="buk"
                     /locus_tag="MJE33_001445"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBY9647386.1"
                     /translation="MKVLVINPGGTSTKISVFQDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFDDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIEIENRIKDGDEKAKLIMDAFI
                     YQVAKEISMYGASLSGNVDRVILTGGISHSEMVVKGVAEKVSYLAPIEIVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            44590..45483
                     /locus_tag="MJE33_001446"
     CDS             44590..45483
                     /locus_tag="MJE33_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBY9647387.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIESISREIGLKGHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMTNALL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPKCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            45513..46721
                     /locus_tag="MJE33_001447"
     CDS             45513..46721
                     /locus_tag="MJE33_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBY9647388.1"
                     /translation="MKYAKRMDMVKASAIRDSQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEITQKVLNERGKFALAYGPTKGEDELLDLLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDVVVTENPSYLGAINAFRPYECDFLGVDTDEEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVNKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIRAEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            46745..48004
                     /locus_tag="MJE33_001448"
     CDS             46745..48004
                     /locus_tag="MJE33_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY9647389.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGVVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFATPTLGMRWVMISLGILSIAMCPVLWFVIKDVDIKEEREEAGEKMSAGDFIT
                     VLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFGA
                     PFGGYCADKLKSPTKVLLPIYVFGIAVIVLFLVLPASTPMTIFIALTFVVGILGYMGK
                     GIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIFQ
                     IAILVIGILSCLYILKVKKRRASKLEA"
BASE COUNT        19266 a         5068 c         8788 g        15195 t
ORIGIN      
        1 aataaataat ttttagagaa tattttagaa gttacgattg tatgattttt aaatgcatag
       61 gcatttactc taggactata gactaatata aaaaaaaact ttgggaatac taaaaccata
      121 aatttataaa attaatattt tatatactgt aaaacagtga ggagggacag tatgcatact
      181 caactacaaa agactattgg tctttcagca gctctttcta ctgttgtagg tatggttata
      241 ggttcgggag ttttttttaa gccacaagca atatacacta caactaatgg agctccaggt
      301 ctaggtatta tagcgtggct tttaggtggt tttataacaa taactgctgg gcttacagct
      361 acagaaattt cagctgctat accaaaaact ggtggtatga tgatttatat cgaagaaata
      421 tatggagaaa agttaggttt tttaactgga tggatgcaaa ctgtattatt tttcccaggt
      481 acatcagcag cattaggagt aatatttgct caacaagcat ctgaactttt aggaatgagc
      541 ccaaataata tggcaaatgt acttcctata gcagttggag ttatactatt tttagcatta
      601 ttaaatataa taggttcatc cttaggagga aaagttcaga cagtagccac tattggaaag
      661 atgatacctt taattttaat aatagtcttt ggatttatta aaggtcaaag tagtgaagta
      721 ttaaatccat ttgtaggaga tggtgtaaat gcatcaaatg cacttggaca agtattaata
      781 gctactttgt ttgcatatga tggttggata aatgttggag ccatatcagg agaaatgaag
      841 tctccagaga aagatttacc aagagcaata gttggtggct tatcattagt tatggcagtt
      901 tatattataa taaatatagc ttatctttgg gtagtaccag caagtgaatt agcaacagtt
      961 acttccccag ctacattagt agcaacaaga ttgtttggaa atataggtgg aaaagtaata
     1021 actgtgggta tattaatatc agtatttggg acattgaatg gatatttatt aacaggttca
     1081 aggatacctt atacattagc tgaaatggga actttaccag catctaaaac tcttttaaaa
     1141 gtaaattctg gaggttctcc tgtaaattca atactactta taactgtgtt agcctgtgta
     1201 tatgcattat ctggtcaatt taatcttttg acagacttaa caatattttc aatatgggta
     1261 ttttatgtat tgacttttat aggtgttatg aggcttagaa gacaaaagcc agatttacac
     1321 agaccatata aagtcccttt atatccaata ataccaatta tagctatatt aggtggactg
     1381 tttgtaataa taaatcaaat attgacttca acagtcatat ctttaggtgg gatatttata
     1441 acactattag gtttaccagt ttattattat atgaaaaaaa gataactact taatttaagt
     1501 tagataaatt tgggtaaagg tgaaaattaa cttttatcca aattttttat actgttatac
     1561 aatataagta ttatatatta cttgatgtgc tattttgttt gatttataat ttagtaattg
     1621 atgttttaag ataatttaag aaatgaggag tgaaagatgc taggacttta tgtacatatt
     1681 ccattttgtg tgaaaaaatg taagtattgt gattttaatt cttataaaat ggacatagat
     1741 tcaaaaaaaa gatatataga agacttaaaa atagaaatgg aattatatag taataaatta
     1801 tataaagata ataaatataa aaataaagaa tgctgtagtc taaataaaaa tgacaaaata
     1861 acgagtatat ttgtaggggg aggtacacct agtatactaa cctctgatga aataagagaa
     1921 gtatttataa gtataaaaga aatgtttgat atagatgaga atgcagaaat aactatagag
     1981 tgtaatcctg ggacattaac attagaaaaa ttaaaaacta tgaaagagat tggcataaat
     2041 agacttagta tagggctaca agctattcaa gaaaagcatt tgaactttat aggaagaata
     2101 catacttatg aagaatttga gaaaaactat aaagatgcat taagtgtagg ttttaaaaat
     2161 attaatatag atttaatgta cagtcttcca aatcaaactc tatgtgattg gaaggaaact
     2221 cttgaaaaag ttgtacactt aaatccaaca catatatcag cttattccct tatactagaa
     2281 gaaggaactg aactatacaa tatgtatgaa agtaataaat ttgaacttat agatgaaaat
     2341 gtagatatag aaatgtatga gtatacaata aactacttaa agtcaaaagg atataatcaa
     2401 tatgaaatat caaactactc aaaagaaggc tataattgtg agcataatat tttatattgg
     2461 gaatgtgaac attatatagg gattggagca ggagcttctg gctatattaa tgagaacaga
     2521 tacaataatg tagaatcttt agaagattat catttaagtt tggtaaaaag agaaaaacca
     2581 atacaagaaa atgaaattct ttctgagaaa gatatgatag aagaaaagat atttatgggg
     2641 cttagaatga ataaaggcat aaaatttgaa gattttaaaa aaaaatttgg aatagatttt
     2701 agagagaagt ataataagca aatagaaatg ctattagcta gaaagcttat aaatcagagc
     2761 tttgaaggta tacaattaac tcaaaaagga agagaaatat ctaatagtgt atttatagaa
     2821 tttatggaat aatttttaag gtgttgacaa aataaattta taatggtata taatatatat
     2881 aatcttttta gcactcgaac ttagtgagtg ctaacagcta ggaggtataa taatatggaa
     2941 ttaaatgaaa gaaaattaaa tatcctcaaa gctatagtta aagattatat agaaacagct
     3001 gaagctatag gttctagaac aatatctaaa agacatgatt taggtgttag tgctgctacc
     3061 ataagaaatg aaatggctga ccttgaagaa ttagggtatt taattcagcc tcatacttct
     3121 gcgggtagag ttccatctga aaaaggctat aagttatatg taaactctct tatgagtaaa
     3181 agcgaattgg atgataatga taagattctt attgaacaat gtatgaatca caatataaat
     3241 catataaaag aattgattca tgaaacttct aagctattat cgcaactaac aaactataca
     3301 actgtagcag ttactaagag tttaataaat caaagtgtta taaagcacat acagttggtt
     3361 gctatgaatg acaataatat tttacttata gttgtaacag ataaagggga cttaaaaaaa
     3421 gctaacctta caactaatgt atatttagat caatctaagt taaatttgat ttctgataat
     3481 cttacaagga agctcttagg taaaagtata acagatttgg atgataatct aattgctttt
     3541 attaaatacg aaataagtga gtattctggg cttatagatg agcttttaaa tgctttgaac
     3601 tcaaatatga aagaagaaga tttttctcta tcattaaatg gagctactaa catatttagt
     3661 tatccagaat ttaatgatgt attaaaagca aagtcatttt taaatatgtt ggaaaaaaag
     3721 gaaacaatag ctgacataat taaatcaaag ggaatacaaa aagacaatct aaatataata
     3781 attggtagtg ataatgattg tgaactggca caggattgta gtatagttac agcaacttat
     3841 aatgtagata gagacttggt tgggcgaata agctttatag gccctaccag aatggattat
     3901 gctagaattt actctataat aaattatatg agtttattaa ttaatagaaa gtaaagaaat
     3961 tttaaattgc gaggtgtaaa gtcttggata aaaaaaatga gcaacaggaa gtaagagaag
     4021 aaaatgatac ttctattaac caagaatcgg aaactcaagt agagttagaa gaggaagttg
     4081 ttaatgaaga atgtgaaaca tcaagtgaaa aaactgatga aaaagaagtt gatgatgaaa
     4141 atgtaacaga tataaattct aagttagcag aaaaaaaatt gcaggatgag ttggatgaat
     4201 taaatgataa atatcaaaga cttcaagccg aatatgcaaa ctacagaaga agaactcaac
     4261 aagagaaaga gacaataggc gtatttgcaa atgaaaaaat aataactgaa ttgataccag
     4321 ttatagatag tatggaaaga gccttagatg catgtgaaga taaggaagat actatgtata
     4381 agggtataag ccttgtacat aaacaattaa tagacacttt agtaaaattt ggagtagaag
     4441 aaatagaagc agaatctaag gaatttgacc ctaatctaca tctagcagtt atgcaagaaa
     4501 gtgtagatgg agttgaagca aatcaaatag taatggtact tcaaaaagga tataagctag
     4561 gaactaaagt tgtaagacct tcaatggtta aagtttcttg ctaagtataa attaaaggtt
     4621 aaattattta acctaatata aaatattaaa ataagtaaaa cttaattata taattactcg
     4681 aggaggattt tagtcatggg aaaaataata ggaatagatt taggaacaac aaattcatgt
     4741 gttgcagtgt tagaaggtgg agaagcacaa ataatagcaa atagtgaagg tatgagaact
     4801 actccatcag tagtagcatt tacaaaagat ggtgaaagaa tagttggaga acctgcaaaa
     4861 agacaagcag ttacaaatgc agataaaaca ataacttcta taaaaactca tatgggaact
     4921 gattataaag taaatataga tggaaaatca tatactccac aagaaatatc agcaataata
     4981 ttacaaaaat taaaatcaga tgcagaaagt tacttaggac aaacagtaac agaagcagtt
     5041 ataacagtac cagcttactt tacagatgct caaagacaag caactaaaga tgctggtaga
     5101 atagcagggc ttgatgttaa gagaataata aatgagccaa cagcagcagc tcttgcttat
     5161 ggtatggata aattagacca agaaaagaaa atattagtat ttgacttagg tggaggaact
     5221 tttgacgtat ctatacttga aataggagat ggaacttttg aagttttagc tacagctggt
     5281 aacaatagat taggtggaga tgactttgac caaatagtaa tagattattt agcagaggaa
     5341 tttaaaaaag ctgaaggtgt agatttaaga aatgataaaa tggctcttca aagattaaaa
     5401 gaagcagcag aaaaggctaa gaaagaatta tcatcaacaa tgagttcaaa cataaactta
     5461 cctttcataa ctgctacagc agaaggacca aaacacttaa atatagattt atcaagagct
     5521 aaatttgaag aattaactag aggtttagtt gaaaaaacta tggaaccaac taagacagct
     5581 ttacaagatg caggactttc tacaggtgat atagatgatg tattattagt aggtggttct
     5641 acaagaatac cagcagtaca agaagctgtt aagaagttta taggaaaaga acctcataaa
     5701 ggtataaacc cagatgagtg tgttgcagca ggtgcttcta tacaagctgg agttttagca
     5761 ggggatgtta aagacttatt attactagat gttactccat tatctcttgg tatagaaact
     5821 atgggtaatg taatgactaa gataatagaa agaaatacta caataccaac taagaagtct
     5881 caaatattct caactgctgc tgataaccaa acagctgttg atatacatgt tcttcaaggt
     5941 gaaagaagta tggcttatga caatacaact ttaggaagat tccaattaac agatatacca
     6001 ccagcacaaa gaggaatacc tcaaatagaa gttacttttg atatagatgc aaatggtata
     6061 gttaatgttt cagctaaaga tttaggtaca ggaaaagagc aaaaaataac tataacatca
     6121 aacactaatt tatcagaagc agaaatagag caaaagataa aagaagcaga aatgaatgca
     6181 gaagctgaca aacaaaagaa agaaaaaata gaagcattca atcaagctga atctactata
     6241 tatcaaactg aaaaaacttt aaatgaactt ggagataaaa taagttcagg tgaaaaagag
     6301 gatatagaaa aagctatagc agatttaaaa gctgtaaaag ataatcaaga tgctacagca
     6361 gaagaactta agaaagctac agatgaagta atgactaagt tccaaaaagt atctcaagaa
     6421 atgtaccaaa aagcagctca agaacaacaa gcggcacaag gtgcagaaca agcacaagac
     6481 aatggaccta aagatgacaa tgtagtagat gctgacttta aagaagtaga tgaagataaa
     6541 taagctatat aatatgtaac actaaatgat aattggaaca tatatgattt gtgaaaatca
     6601 tatagatgtt aaagatatat ggacaatata catcataaag aatataatat atcatagatt
     6661 atataactta aagtagaata taagaattaa tgatagcttg tagtttatta ttctacaagc
     6721 tatcgttttg taaaatgaag gtggtgtcaa tttttgagta caaaaaggga ttattatgaa
     6781 gtcttaggta tcagtaaagg tgcagaagct caagagataa aaaaagctta tagaaaatta
     6841 gcaatgaagt accatccaga tagaaatcct ggagataaag aggcggaaga aaaatttaaa
     6901 gagataaatg aagcttatga agttctatct gatgatacaa agagaaaaac ttatgaccag
     6961 tttggacatg atgggctaaa tggtcaaggc ggttttggtg gtcaaggagg atttggcggt
     7021 caaggctttg gtggttttga agatatgttc ggagatatat ttggtgacat gtttggagga
     7081 agcttcggag gtggaagagc aagaagaaga ggacctcaac gtggtgcaga tataagacag
     7141 agtgttacta tatcttttga agaagctgca tttggtaaaa agatgtctat aaaagtaaat
     7201 agaagtgaag agtgtgaaga atgtaatgga actggagcaa aaccaggaac atcaaagaaa
     7261 acttgttcaa cttgtaatgg aactggtcaa gttagaactg ttcaaagaac tccatttggt
     7321 aatatagcaa gttcaagacc atgtagtgct tgtaatggaa ctggagaagt gattgaatct
     7381 ccatgtagta aatgtcatgg aactggaaat acaagaaaag ttaagactat agaagtagat
     7441 atacctgctg gtatagatga tggtcaaatg ataaaacttt ctggtcaagg tgaagtagga
     7501 gaaaaaggtg ctccaagagg agacctatat attgtagtta atgttaagtc acatccacta
     7561 tttactagag atggaaatga tatttatttt gaaatgccaa ttacatttgt tcaagcaact
     7621 ttaggtgatg agatagaagt acctactttg gatggaaaag ttaaatatag tgtgccagag
     7681 gggacacaaa ctggaactgt atttagactt aaagagaaag gaatccctag aattagaggt
     7741 aattcaagag gagaccaata tgttaaggtt gtagttgaaa ttcctaaaaa attaaacgat
     7801 aagcaaaaag aactattgag agaatttgcg aaggagtgtg ggtcgaacgt ccacgaaaag
     7861 aagaaaacct tcggacaaaa gatagaagat atgtttaaga aaaagtagtt aaagtagcca
     7921 atatataatc atatttttaa aatcttagtt atttataaga ttgtcaaagg agatattatg
     7981 atttttatat tggctatttt tgtataaaaa ataaatgtgc ataaaataaa actgaatata
     8041 aataattttc tgaaaaatac ttgtcctaaa atatataatg tgctataata taatcaaata
     8101 aaagaaaggg gtgattccag tgggaacgga tgtggcactg ccaaatgatg aagaaaactt
     8161 tattgtaata tacaaaataa aattagatac ttttagtgct agattaagta ctggaaaaac
     8221 tcatgagctg ggataattat tgtaattacc tagactcaaa cttttagtag taaaatagta
     8281 atattatata gacaaggtat ctaattttta aatcttttgt atgaattaaa atttttataa
     8341 agggtatatc tgattagaat gtaattaggg aaacctagtt gtgaattact tttagtatat
     8401 acatgtgtaa agaaatatgt atatggtatt ggttattgtg ctagatgtgt atgtaaagaa
     8461 atatgtgtct agttgggtat atgtaaagaa atatacttat aatctatgaa aaatttttag
     8521 tataaattgc taaaacattg agattgtttt ttatagaaag ttagtttata ccaactaaaa
     8581 atgtgcttaa gatttgtttg atgtttaatt ataaataagg ttgtagataa aaaaataagt
     8641 aaagacaaag gtttaaaata cttgctttaa atatagtttg ttttgaacac ttgccaaatt
     8701 aaaataatta ataaaataga catcttctca tagtaaaata tactgtgaga ggatgttttt
     8761 gctttgttca taaactttta tgaaaaattt gcatagtgtc acattttaat atactatata
     8821 tataataaaa tatagataaa attaaatgat tcaaaagaga aaggtgaaaa gagatgtatt
     8881 atataggtgt agacatagga ggaacaggta tacaagcagg agtagttgat aactatggga
     8941 aaataatttt taggtcagag tgtaaaacag taattgaaaa aggatttgaa ggaatactta
     9001 atgacataaa aataatgatt tataagttgt tggaagataa taaacttact atgagtgaca
     9061 taaaatctat tggatttggt gttccaggtt ttataaataa agaaggatta gtaacttgtg
     9121 taaatttaaa atggaataaa aaagctttta ataaagagtt aaaaagaaga tttccagatg
     9181 ttgaaattca tggagaaaat gatgcaacag tagcagcact tggagaggca aagtttggaa
     9241 gtatgaaagg tgctaatgtt ggagtattat atactttagg aactggtgta ggtggaggaa
     9301 tagtaattaa tcaaaaagtc ttttctggag cacatggatt gggttcagaa ataggtcatc
     9361 aaattatagg agaaaactat tttaattgta attgtggaaa taatggttgt gtagaaactt
     9421 tttgttcagc aactgcaatt ataaagtatt ctcaaaaact gatagaagaa ggagaaaaga
     9481 gtaggatttt agatttggca gagggaaatt tagaaaatgt aaatgcaaag atggtgtttg
     9541 atgcatatag agaaaatgat ttagtggcaa ttaaagtaat aaatagattt aaagagtatt
     9601 tagcaaaaac ttttgcaaac acaataaatt ctttagaccc agaaattata tctataggag
     9661 gaggcatttc taaatctagc gatataatac ttgatggaat agaagatttg gtaagaaaat
     9721 ttgtattata caaaacagaa gatatagcta caattacatg tgcaacttta ggttcagacg
     9781 caggaattat aggagcagct tttttataat gtaaatgaac tgcttattgg aggcgtgatg
     9841 atatatgggt aaaacaaatg ctagaagaac gctttttttg atagcaatag gttcaggaac
     9901 tatgctgaat cctcttaatt cttctatgat ttcacttgct ttacatagta tacaaaatga
     9961 ttttcatatt agttttacaa ctgtatcttg gattgtttca gcattttacc tagctagtgc
    10021 tattgcacaa ccagtttcag gtaagatagg agatttaata ggtagaaagg tcttattttt
    10081 gagtggtttg atgcttgtaa ttttatcagc atccatagta ccacttgttc aatcattctt
    10141 tatattgatt tttataagag taattcaagc aattggaaca agtaccctat atccatctgg
    10201 agttgctctt atacaaaata atataaaaga aagacaatct tctgcattag cagttctaac
    10261 catttttgct tcaactacag caggattagg tccgacactt ggaggattat tgttggactt
    10321 aggaggatgg catgctatat ttcttgtaaa tatacctgtt gttttggttt ctatatgctt
    10381 agggtatttt ttattcccaa aagaagtaaa agaaaagaaa tctataaaag aaactttaaa
    10441 aaatgttttg tcaagattag atattattgg catacttcta ttttcaattg caatgatttt
    10501 tatattacta tttttgctat ctatgaagga gtcttttaac ttagaacaac ttatatttgg
    10561 tataatactg atgtgtcttt ttatttggca tgaattgaga acaaaattgc catttataga
    10621 tattaagctt tttgtaagta atcctaaatt gtcaaaagtt tacttgcaat ttattatatt
    10681 aaatttcttt aattatattt tgttttttgg tcttcctagt tattttcaag atgccttaca
    10741 ttatagtgca aagtcgactg gattatttat gctatttatg tctggtatag gtatatttat
    10801 atctccatta actggaaaat ggatagataa atctggaact agatttcctc ttgttacatc
    10861 atcaatcttt atgtttatta gtgcagtact tctttcagta gtttttatcc atatacctgt
    10921 aataggaaag ggtattatat tatcattagc aggtattagt tatggagttg gtaatgtagc
    10981 cctgcaatcc tctatgtttg aagaaagtcc tcgtgacagt atagggacag cttctggttt
    11041 gtttcaaact agtcgttatt taggttctat tttagcaact gttttacttg gaatggtatt
    11101 tgaagtaaat ataacatcag aacaatttca gatattaggt tatgttatgg tggtcttagg
    11161 tacaataagt tttttattaa attttatatt aaagaaagaa ttaagaaaag tagaatagtg
    11221 gatttatgga tttaataagt ctaataactg ataagtttaa aaatattgtt tgtaaaacat
    11281 aactggtttg gtatttatta attctaagtt ttgaagcata gagtactcga taagtataca
    11341 atattttgaa ttaaaattaa gcagaaattt gaactagtct ttcaaatctc tgcttaattt
    11401 agtagttaaa tttttttata gagaaaataa gatttgacac ttagataaaa aatcattgca
    11461 ttttcaaaat tctttaaatc aaacccaagt atctcattaa tcttttccaa tctatatact
    11521 agcgtgtttc tatgtaaaaa catactagta gatgtcttag aaatatttaa atcattattt
    11581 ataaatttta ttcctgtata tagaagttct tcgtcaatag atttaatctt attaaaatct
    11641 attaatttat tgagtatatt atcctcatta attaaatcta aagttttata tctaataagg
    11701 tcaaacaaat ttaaaaactt attttcagcg ttgtctttta taatatgagg tagtaaatca
    11761 gaacataact gtaatctaaa atcaaaatca gctttactat ctaatttacc aagataaaaa
    11821 atataagtac tttgataagt ttcagtttca attatatttt gtgtagttac attaatatct
    11881 aagttatatt tagaatttac tataaaaaaa ccattgtgtt gtattatatt tatatcttca
    11941 aataaattat aaaaaatatt ttttagatat tcaaagtcta tatgagattg gtaaattaaa
    12001 taaaagttgc cttttaaatt atcgagatta tttttatcta gaaaatcaat aacataaata
    12061 gcattattga aagtaactaa atggctacta gcatctaact tttggttttc atatggatag
    12121 atatttattt ttttattaag ctgtctttct aaaaaatctt ttaactcttc cataaagtct
    12181 ccttaaaaat aagattatat tcttatctta tagctaaagt agtttctttg tcaaatatgt
    12241 gtactctact attgtcaact gaaagtgtta agtcatcacc aacattaatt ctgtttttac
    12301 tgtcaaatct aattgttatc gtattttcgc ctagttttaa gtgagcataa gtttctgctc
    12361 ccataagttc ttttatttca attcttgatt taaaacaagt atctggatta gatttaataa
    12421 aagtatcttc tacatgaata tcttctggtc tgatacctat aacaacttca ttattaatat
    12481 aattgtcttt tattaaatca tagtgttttt ctttattcaa tttaagctta atatgttcat
    12541 tttgtgcata aatttcatca ttttcttcta ttaatattac atcaataaaa ttcatctgtg
    12601 gtgctccaat aaatcctgca acaaatatat tagctggata atcgtataca tcttgtggag
    12661 ttgcaatctg ctgaactact ccatccttca ttactacgat tctatcagcc atagtcatag
    12721 cttcaacttg gtcgtgtgtt acatagataa atgtagttcc aagctgttgg tgtagcttag
    12781 ttatctctgt tctcatagca gttctaagtt ttgcatctaa gttggataga ggttcatcca
    12841 ttaagaatac cttaggattt ctaactattg cacgtcctaa ggcaaccctt tgtctttgtc
    12901 ctccagataa tgttttaggt tttttattta aaagaggtag taaatctaat attttagcag
    12961 cttctttaac ttttttgtct atttcatctt tgggtaattt tctaagttta agtgcaaatg
    13021 ccatattttc gtaaacagat aaatggggat ataaagcata gttttgaaaa accatagcta
    13081 tatctctatc ttttggttct atatcattga caagtttatc tccaatgtat agttctcctt
    13141 cagagatttc ttcaagacca gcaatcattc taagtgtagt cgatttacca caaccagatg
    13201 gtcctactaa aacgataaat tctttatctt taatgtctat atttatattt tttactgcgt
    13261 taaatccatt tgagtaaagt tttgatatat tttttaaaat tacttctgac ataatatcct
    13321 ccttgatatt taaattttaa ttatatagta tcaaattttt ttgttatatt aaatgtgtaa
    13381 aatgactaaa tgtatttgtt taatatgtat atttagtttt tagcaaagaa taaaactaac
    13441 agtaaagtat atttaaaaaa ttaaaaaaca aaattaaatt tattatcaaa aaatcatttt
    13501 atgatatgtt ttatttttat attacaaata gagaataatg aattatacta gaaagtagga
    13561 ggtgaaaata tgagcagaat ttcaattttt atggaatcat gtaatggcaa atttccaaat
    13621 gaaaattcaa tgttgtcagt ttcctttata aaaaatattt taaatgcaga aaacaaagat
    13681 tttgctaaaa acatatttaa ctatgggaaa gaaagaaaaa acaataagca aataaaggat
    13741 ataaatacag ccatttacat accaaatctt ataagaagca aaaatgagtt acttgttgat
    13801 ggagatataa tattcaatat aagtttttat aattattcta tgttcagtaa attatacaat
    13861 ggaattttaa aagtaaagga gctagaatat aaaggttata gatttaaaat aaaaaatata
    13921 aaaatacata aagaacacga gataaaagaa aatggtgtta tatttaaaac tatgtctcct
    13981 ataatagtaa agaataaaga aggtaaatat ttagatgtag aagattcaaa ttatatagaa
    14041 tgtttaaact atatagctaa tctaactctg gagagtataa gaggtagtgg gcttagaaag
    14101 ccattggaat ttatacctct taacttcaaa aaaagagtgt taaaagaaaa gattcgtgga
    14161 tttaaagtga gagaatttta ctatataaat gcttatgcag gaacattttt tttaaaagga
    14221 gatatggaag atttaaatgc actgtataaa atgggaattg gatatagaag gacggaaaat
    14281 gcaggaatgg ttgatatatt aaagtagcga ggtgagagaa ataaaagcta aattttatag
    14341 atcaggtgat attgtaagag atattggcat tgtttacttt tatgaattac taatggattt
    14401 aaaaaacgaa ctagaaaaaa atgattataa gctaaaattt acttgtgagt taaatagaaa
    14461 ttatttatct ttagaatctg cagaatatat agaccctaag tatatatctg attatatatt
    14521 agaaaatcaa ctttttaagg tatttaaaaa tgggaaaaag gaaaccttag aaaaaatatt
    14581 tagtaatata gataatttaa cgtatgtaaa ttttattgaa gaattggata aatcttctgc
    14641 aactgctaaa caaaaggaag atattaaaaa agattttaaa aacaggaggt ttccatatgt
    14701 tagaaatagt ggtaagtatg gattaaacac gagtttagaa aatatggaaa caaatgtaaa
    14761 aagaatagta gatactgtaa ttaaatcaaa tatagagata gatgaaatag ataaacttga
    14821 tttgacacaa tatgaagaaa aagatagtgt atgtaatgtt tgcaatataa ataaaacaac
    14881 aaaactagat attgatttaa gagaacgaaa ggactctaag tataactttt tatttagagg
    14941 tgcagaaaaa tcaggcttta aaagaagtgg acaagttgaa agtaatattt gttttgagtg
    15001 tgaatttttc aacttaatgt gtcttttata tataaattta aaaagaccaa tggtttttgc
    15061 atatacgaat gatttaagag aattagcatt tttaaatcat aagataatgc ttaaaagaaa
    15121 aatgtattca gataaatcat tttataaaaa acttttgcat gaaaaaataa gttcaattag
    15181 actatataga tttgatattg ttaagaataa aggtataatt ctaaagtttg actctattat
    15241 agagtataaa gaattattaa aaaaaattga acttatagat attatagata attataattt
    15301 ctcaagggag ccagtaaaca ctaaaaattt aggtaaaaaa atgattgata atggaaattt
    15361 atcaaattta aaagaattat tacttgataa tataagtttt ttaaagcaat caactggaac
    15421 ttctcgagaa atggattttg tatctagctc atataatatc ggtttatata taaaattgtg
    15481 ttggattgta gatggagggg aggagtataa aatgaaaaac tatatggaaa gtaatttaat
    15541 atattcaaga gtaggtaaag acttatttaa taaaatgaca gatgaatcca gaagaaaaaa
    15601 tttttctatg aaagtgattc aactacttaa gtctaatgat agaactcagc tatttcaaac
    15661 tataatgcat gtgttagttt ctaatggaat cttaatagga gaagggtttg tagaaggtct
    15721 aatgcagagt tctgaattag aattaaatac gaatgtaggt ctatttattc aagaaatcat
    15781 gaaataaaaa agggggaaaa tttaaaatga atgaaaaaag agtaaaagga gtaacattaa
    15841 ctatactgac agaaagccca gttccacttt catatgacca agggtatgga aattatacac
    15901 caataaaaaa agaacaatat agagaaaaaa tacatgcaaa aactagtata gccacaataa
    15961 catatgattt aagaagaatg ctccaccaag aatatggttg gaatttaagt aatattgttt
    16021 ttggaaaaaa aggaaatata tatccatcta ttaagaaaaa tgtaggatgt gtagatgaaa
    16081 atggattaga gactgatgta tttggttatt tgataccact taaagataaa ggttctataa
    16141 gtaaagcttc tccacttaga ataatacctt ttaggtcatt aaatccatat ataggttcaa
    16201 cacagctaat aacaaataga ggttttttaa gtagcgaatt tggtagaaaa tattatgatg
    16261 agaaggaaga aaatgaagta cctagagatg aaaactttcc aacaacacaa gcattagcaa
    16321 tagaagagac tttatcagat tactacgttt atacaataac attggagctt gatagaatag
    16381 gagtagtaga agtagaagat gggaagttac ttttaccaga agaaagaaaa tttatgagta
    16441 aggaattaag agaaaaggca gtaaaagata tattagatgc aattactgtg tttacaagaa
    16501 acataaaaca ttcatcagta ctattaaaac cacttgcagt aataggtgga gcttttgata
    16561 aagttgtacc atttttttgg gatgatgtag attataacgc tgatagtggt gagataaatt
    16621 tagagggagt catagaaact atagaaagtt atagccttaa agaaagtaac acaatacttg
    16681 ctatcaatga cagattaaaa atttctaata aaaaggaatt aaataagttt aatttaagta
    16741 aataccctgt aaaagagatt aaaaatctag cagatagatt ggaaattgga gaagataata
    16801 tgtggtattt aaaagagtag gaggtttttt gaattatgaa agtattacaa tgtaaactta
    16861 agcaaccaac tgctcactat agagacccaa aagtatttca aaatgaatat ataagtactt
    16921 taaatttacc atctaagacc acaataatgg gaatgataac gtatttatgt gatagaagat
    16981 taaactctga tatagatata ggaataattg gaacacatca tcatagagaa ctagaatttt
    17041 caagaggaga gaacattgat ttttggaatg aatatacaaa tatgagaaaa ggcaaagata
    17101 aagaaaagtt tttgcttcaa ggaaactact atgattatta taaagaacat aaagctcaaa
    17161 atagcatatt aaactatgaa gttcttaaag aagtagaact gactatattt atttcttgca
    17221 aggatgatga agaacttgaa tttattaaaa aaaagcttga aagtccatgt aaatatgcaa
    17281 atttaggaag aaaagaagat tttgtaatac caagtgaaaa agggtgtttt gtgaaagaag
    17341 ttgagttaaa agaggttata cctatgaata ctcgtgatgc tataaaagaa aatataaaat
    17401 taaagaatac atatgtaaga gttgacttaa gagataaaga gaatgtcgag actataataa
    17461 atcaaggtgt attgattgca ttacctcata aatataaaga tttagaagca aatagagatg
    17521 atagagttta tgagttttgt cattatatat atgttgataa tgatggtata tatcctaaaa
    17581 atattaaggt aaatgtatgt attgatacaa aagaagtttt tacatggctt taaattattt
    17641 tgaaagatag gtgtttatca tgaaaaatat catgcaaaaa gaaatattag ctaagtcata
    17701 caatttaaga actaatcaac aagagcaaac attagttgaa catattaagg atttatttga
    17761 agtattagaa tctatattag aactaaatct atattcagat aaagatgtag agatattgaa
    17821 aatatgttgt gctttgcacg atttaggaaa aattaattca attatgcaac aaaaaatgga
    17881 gattaataat aaaatatcgt atagttgctc agaagaagag aggaaaaagt tagagtctga
    17941 caaaaaatct ttaaaaaaga tagcaagaca taatatattt tctggagcat ttttaaaaga
    18001 tatattagaa aaaatgaact tgtcagaaga agataagtta tatatatata aaagtataat
    18061 gttacatcat ggaaactatg aagattatat gaggttaagt attagtaaag ttcaagaaga
    18121 aatttatgag tatattgaaa aaggcatttt agaaaaagaa gagtttaatc tgaaagatat
    18181 agaaagttat ataaataaca ttttaaatgt agactttaaa tttggtgaag atgtattgga
    18241 ttatgattac atagataaat taagtgaaag tatgtttata gacagtgatt ataacaatgg
    18301 tcaaatagat gattcagttt taaattatag aaagtttaag tatattttat ataaagggat
    18361 gctgaactta atagaccata gtgcatcaag tagacagaaa ggtataaaat tttataacga
    18421 ttttactgat gaagaaattg ataatatgat tttaaatgag atatataaga gtcagggtaa
    18481 tttagaaaaa aataatatag agttcaatac tatacaaaaa aggttgagag aactttctgg
    18541 aagaaatgtt ctgactgttg catttacagg ttcaggaaaa acagctgcag attatagaaa
    18601 gacttttaaa agaaaattct ttttagtgcc aaataaaata tcagcagaaa gtttttatag
    18661 aaaaaatatt tttcaaaata aaaatgattt agatacaaga agtagtaatg attacattgg
    18721 attacttcat ggagatataa acttatattc agagaatgaa gataatggag aacatgattt
    18781 tgtattgaca ttaagggata tagatttgag tataaacttt tgtaaacctt gtgtaattgc
    18841 aacagttgac cagttattac ttagtatgtt taaatttccg ggatatgaaa aaatattcgc
    18901 agccgtaaaa aatgcatcta taactgttga tgaagtacat ttattggaac caaagatgtt
    18961 tttgattttg atatatttta tgcagtttgc atgcaagtac ttagatgttg attttcactt
    19021 aatgacagct acacttccaa aatcatataa agagcaaatg ataaacaagg gaattatttt
    19081 tcaagaagaa agtaataaaa atgttactga taaaggagaa atagttttta ttgagagcaa
    19141 caaggaagaa gagatttgtg aaggtaaaga tgtaaaggtt tcttttataa aggaaaaaga
    19201 aatcaagtca atagttgaag atgctttaga aaataaacaa aaaattctta taataaagaa
    19261 tactattgat tcagtcaata gaacatatga gtttttaaaa gaaaatttat ctgataagta
    19321 tggtggtgta gatatagatg ttttacatag tagatttaag ttcaaagata aaaaagagaa
    19381 atatagtaaa atattaaatg gagagggtga tatttggata tcaactcaat cagtagaaat
    19441 atccttggat ttggatttca atataataat aagtgattta gcaactatgg atagcttgat
    19501 acaaagaatg ggtagatgca atagaaataa taaatatgaa tatggaaact tttatattct
    19561 acctagtgaa gataaaattt atgataacaa acttaagaac acaactaaaa acatcttaaa
    19621 ggacatatta aaaactaact ctatttttac aatgggaatt agaaaaacta ttttggatga
    19681 ttactatgat aatagtgtag tcaaaaaata ttttgaagag aattttataa gttgtgataa
    19741 agaaattaag aatatttatg gtataaataa agaaattttt gatggattag atttaatttt
    19801 taattttgaa ccttacaaaa atattgttga tagtaagaag gaagcagcta aaatttttag
    19861 agatattgat gttagttaca aaattattct ggaagaagat ttttataagg aagataggga
    19921 gttacaacaa gattcgattc aagtatcagg gtttatattt aatagattat attattatgg
    19981 attaataagt aaagtagaag gatacatggt actaaaatct agtagtaagt ttgagtataa
    20041 tagtactgta ggcttaattt tgaagtgagc atatgtttaa atataatata cctttagcca
    20101 ttgaaaatac aaaaagtact actgtaagtc cactgtaaaa actatttttt aaaaggaaaa
    20161 ataagttgtg agatagagtg tttacaagtg tatatagata ataaatttga ggtttaagat
    20221 taactaagtg ggtttcaggt acatcgcgaa aataattata agttggagat tttgtatgtt
    20281 taagattaac taagtggatt tcaaatattg atatagtaga tataatagaa gttttattat
    20341 taggtttaaa attaactaat cggattttta aatattctga ctattatata aggatattat
    20401 tagcttgaat ttaaaaccaa taatataaaa caacaaaata ttatatttat gatataataa
    20461 aagttatgga aaaacaggaa tattactata gagaagagag gtaaacttat gaataattgg
    20521 atagaagtaa ctataaaaac aacaacagaa gcagttgaac ctataacgaa tatactatat
    20581 gaacaaggag ctggaggagc tgtaatagaa gacccaaaag actttttatt ccaaaagaaa
    20641 aatgaactag actgggatta tgttgaagaa gaagtattta aaaagaatga agaagatgat
    20701 gttcttataa aaacttatgt ttctgaagaa aaaaatgtga tggaatttgt cgaaataata
    20761 aagcaaaaag ttttaggatt aaaagatttt ggtatagata taggtgaagg aagtgtatca
    20821 ttggaccaag taaatgaagc tgattgggca aatgcatgga aagcatacta caaaccaaca
    20881 aaagtaggac aaagggtagt agtaaaacct acatgggaag attatgctat gcaagagggg
    20941 gatttaatca tagagcttga cccaggaatg gcatttggta cagggacaca tgagactact
    21001 agtatgtgca ttagagaatt agaaaagtat gtgaataaag attctaaagt atttgatatt
    21061 ggctgtggta gtggaatcct tgcaatagca gcagctaaat taggagcaaa agaagttgta
    21121 gctgtagatt tagatgaagt ggctgtaaaa gttgcaaaag aaaatgttct tgaaaataaa
    21181 gttgaaaaaa gtgtatctgt aatgcatggt aatttaactg atgtaataaa agataaagcg
    21241 gatgtaatag ttgcaaatat aatagctgat ataataaaga tacttgcaaa agatgttcaa
    21301 aactttatga aagaagatgc tatattcata tcttcaggta taattttgga taaagtagaa
    21361 gaagttaaag aaagcttaat tgaaaatgga tttgaaatag ttgaagttca aaaattaggt
    21421 gaatggtcag caatagtttc taagcttaaa aaataaggga tatggtgacg tataatggat
    21481 agattttttg ttgaaaaaaa taatataaat ctacaagata aaacttgcac tatagaaggt
    21541 gaagatgtaa aacatatttc aaaagtttta agatgcaaat taggtgagaa acttgaaata
    21601 tgtgacaaga ataataatga gtatatttgt gagataatga atatagataa gagtatagtt
    21661 aatttagaaa ttttagaaaa agttgatata aatagagaat cagaacttaa agtaaggcta
    21721 taccagggac ttccaaaagc tccaaaaatg gaaatgatac ttcaaaaatt aactgaagtg
    21781 ggtgtagaag aaataatatt agttcaaact aagagaagtg ttgttaaagt tgatgacaaa
    21841 aaagaagata aaaaatttga acgttgggaa agaataattt atgaagcagc aaagcaaagc
    21901 aaaagaggta agataccaaa attaagaggt gttttaagtt ttaaagaagc tttagaagat
    21961 atgaaaaaaa ataatgtaaa tatttgccct tatgaaaatg aaagaacagt ttcaataaaa
    22021 catgcactaa aaaaatgtga ttctaatata gatagtgttg gaatttttat aggtccagaa
    22081 ggtggatttt ctgaagaaga aatagaacaa atacaaaaaa ataattgtaa tgttgtatca
    22141 ttggggccta gaatattaag aactgagaca gcttctgttg tagcatcaac aatagctttg
    22201 tatgaactaa gtgatttagg aggagaaaaa taaattgaaa aaagtagctt tttatacact
    22261 tgggtgtaaa gttaatcaat atgaaacaga agctatgcta gaactatttg agaaagatgg
    22321 ttatgaacaa gtcaatagtg aagagtatgc tgatgtttat gttataaata catgtacagt
    22381 tactcatatg agtgatagaa aatctcgtca atatataaga cgagttaaaa agaagaatcc
    22441 agatgcaata atagccgttg taggatgtta ttctcaggta tctccggaag aaattttgga
    22501 tatagaagaa gtaaatttag tcatgggtac aaatgataga agaaagattg ttgaagaaat
    22561 taaaaagata aattcaagta agaaagttag tacagttgac gacataatga aagttaaagc
    22621 ttttgaagaa atagaaatca gccaaactaa tggaaagact agagcattta tgaaaataca
    22681 agatggatgt gacagatttt gtacttattg tatcattcct tatgcccgag gacgcgttag
    22741 aagtagagat attgatagta tagtcgatga agtgaaaaaa ttggctaata atggctataa
    22801 agaggttgta ttaacaggaa tacatgttgc ctcttatgga aaagatttaa aggataggga
    22861 tattaaactt cttgatgtaa taaaacaaat aaaccagata gaaaaaatag aaagaattag
    22921 gcttagttct gttgaaccaa ttttatttac agatgaattt gtaaatgaag ttttaaagat
    22981 ggataaagtt tgtcctcatt accatttatc acttcaaagt ggatgtgatg aaactctaaa
    23041 aagaatgaat agacgataca caactttaga gtacaaaact attgtagata gattgagaag
    23101 taaaatgcca gatgtggcaa taactacaga tgtaatagta ggttttcctg gagagacaaa
    23161 tgaagagttt aaaaaaacgt acgagttttt aaaagaaata gagctttcac aaatgcatat
    23221 ttttaaatat tcaccaagaa aaggaactcc agctgcaact atggaaaatc aagttgaccc
    23281 tcaaatgaaa cactttagaa gtgaacaact tttaaattta agtaaagtaa attttaataa
    23341 atttgcaact aaatttatcg gccgtgagtt ggatgttttg tttgaacaaa atatagaagg
    23401 caataaatat gagggattaa cttcaaatta tataagagtt gttgttgaaa gtgacaaaaa
    23461 tatacaaggt cagatattaa aagtgaaaat aaatgatgta aaagatgaat atgtagaagg
    23521 aattttgcta taacatgtag aaattataat atttataaga taggaggtgg aaagaatgga
    23581 ttgtattttt tgtaaaatag cgaacggaga aataccttca actaaagttt atgaagatga
    23641 tagagtctta gcttttaatg acttgaaccc agttgctcca tatcatatat tagttgtacc
    23701 taaaaaacat tatgatagtt taattgatat tcctgataaa gagatggaca ttgtgtcaca
    23761 tattcatgtt gttattaata aaatagctaa agagaaaggt tttgaccaaa ctggattcag
    23821 agtcataaat aattgtggaa gtgatggagg gcaagaagtt aaacatttgc actatcatat
    23881 tttagctggt aaaaagcttc ctaattacga agcgggacaa aactaaacta aattattgaa
    23941 aaattgagtt ccttcttgat aaaaatactt gataaaagta aaaatttagt ttataataaa
    24001 caaagtgtta ggttgtaatg catattatgc aaaaagaaca tagttacagt cccagcataa
    24061 ttgcgctatt ggagggaggg aaaaagaaat gtcagaagta agagttagag agaatgaaac
    24121 attagacagc gctttaagaa gattcaagcg tcaatgtgca atgtctggca tcatgtcaga
    24181 agtgagaaaa agagagcact atgataagcc aagcgttaag cgtaagaaaa aagctgaagc
    24241 agcaagaaga aaaaacgcta agaaatagta gtaaataaga ataaagaggt gaaggggatg
    24301 tcccttaagc aaaagttaca agaggatttg aaatcttcga tgaaaaacaa agatacagtg
    24361 agaaaatctg tagtaacttt aataagagct tctataaagc agtatgaggt tgataatagg
    24421 gtcgagcttg atgaagatgg aattatcgat gtaatagcaa aacaattaaa gcaacgtaga
    24481 gatgcattag ttgaatttga aaaagctgga agagaagact taataaaaga aacagaaggt
    24541 gaaattgaag ttttaaaaga gtacctacct caacaattaa gcgaagaaga gttagaagaa
    24601 attgtaaaat ctactatatc tgaagtaggg gcaacttcta tgaaggatat gggaaaaata
    24661 atgtcagtta tccaaccaaa agttaaagga agagctgacg gaaaacttat aaataagttg
    24721 gtaaaacaaa acttacaata gattgatata gcctagagag aaatctctag gctttttata
    24781 gtttttggga ataacgagtc atatttgagc atatctataa tcaaagaaaa ttttgaaaga
    24841 aggggaaaaa tttgattata tcagtaaaag agattaggaa ttttttgata aacatattta
    24901 taccactaat aataggttac ttaagtagca tcttaacaat gattatcagt ggaacagata
    24961 tttcaaccta ttatttacaa ctcacaaagc caagctttgc accaccagct tttatctttc
    25021 ctatagtttg gactatattg tacatattga tgggtatatc ttcataccta atattaagaa
    25081 aaggttacaa cttacctaaa gtaagagatg caatttttta ttatggatta caacttgtat
    25141 taaactttat ttggagtata ctattttttg gatttggact tagatttaca gctttgatag
    25201 atataataat aatgattttg gtttctataa taatgatttc gaagttttct aaaatagata
    25261 agagatctgg atacataaat ttgatttatt taatgtggct tgtttatgca ggatttttga
    25321 actattttat atggtttatt aataaataga atgtaaagat tttgtaaatt accctgtact
    25381 tttaataata tcataacaaa cgaaaaataa atagactatt aaaaataatc attttcatat
    25441 acaataatat taatttaaat acaataaatt tataaaaatt gtatttaaag attaacatat
    25501 taaagtatta gaattggaaa ggcttgaagg gagaggtttt tatgcaaata atagtatctg
    25561 ggagacaaat gaaattaact gacggaataa aaggatacgt agatggaaaa ttaagtaggt
    25621 tagaaaaata ccttgatcct gaatcagaag tgaaagtaac agtaagtgcg aaaaaggata
    25681 gacaaaaagt ggaggttaca ataattccta ttaacggtca aataataaga gcagaagatg
    25741 tagaagatga cttatatgca gcaattgata ttgtttgtga caaattaagt agacaagttg
    25801 tcaaatataa aactaaagtt aaagacaaag ttcaaaataa taagagcata agatttgaaa
    25861 atcttgactt tatagataat agcagtgaat ttgatgatta tgattatgat gatgaagaag
    25921 atgaaaatat agtaatagaa agaagaaaga aatttaatgt aaaacctatg agttctgagg
    25981 aggcgatact tcaaatggaa ctagtaggac acaatttcta tatgtttaga aaccaagata
    26041 attttgaaat aaacatagtg tataagcgta aagctggtgg atatggttta atagaacaag
    26101 attaataaaa attgtatacg tttagatttt atataagaaa aaataaaaag gtaatcatgt
    26161 tgatttttta tcaatatgat tacctttttc ttgtttaaat aatatattag atattaggta
    26221 tttattatat aaaaatttta aaactaatct gttacaacat caataaagtg cataaatacc
    26281 tatttcaagc ataaaattaa ttaagggggt aatgcttatg ttagaaatat ccactgattt
    26341 acaagtaaat caaccagtta ttactgttac aagtaatacg tttattagta tagaaaatta
    26401 tctatcaata ttagaatatg aagttgattt aataagaata aagactaaag ttaaaacaat
    26461 taaaatatct ggggataaac tttcacttaa gtatattact gatagtgaga taggtataaa
    26521 agggattatc tacaatgttg agtatgttga ttaggaggag atacacttga taagttttat
    26581 aaggggatac tatgttatag ttgtggaagg agtaggactc gaacaatttt taaatcatct
    26641 tataagaaat gggataagtg tttacaatgt aactagaata aaaaatacta aaatggagtt
    26701 tcatatagat agacaagata taaaagactt taagaatgtt tatagaggaa gtaagtttga
    26761 tataaaggta aagcaaaaaa ctggggtccc ttttattatc aaaagagttt ataagcacaa
    26821 aggtatgtgg atttgtgctc ttgtttcttt atttttattg atgtctacat cacagtttgt
    26881 gacagatgta tatatacaat ctcctgaagg tataaaaaaa gaagctctta gaaatgagct
    26941 ttataaggtt ggagtaagac caggagtcta taagaagagt attgatagaa aagaagttag
    27001 agaccatatg atgtcaaaat ttaatgatgt ggcatattta tctatcaatg taaaaggaac
    27061 aaatatattt gttactgtta caaaaaaggc agaatcccta aaatctacag accaatcaaa
    27121 ttattgtaat gtaattgctt taaaaaatgg tataatagaa aaggtaatcc ctagaagtgg
    27181 taaatcagtt attaaatctg gagacatagt ccaaaaggga gatgtcttat taaatggagc
    27241 taataccaag tcaataccag aagtatgggc aagtactttt tatgagtcca caaaaaaggc
    27301 aagttatgta gatactgtaa ataaaaaaac tggagaaaag aaaaatatat atacactaag
    27361 tttttatgat aaagagttta caatgagaaa aaatataaaa tataaagatt atgttgttga
    27421 aaataaagaa aaaaaactat caataggaaa ctatactttt ccaataaaga taaaaacgag
    27481 tactttttat gaaacaaaaa aggttgaagt aaaaaggaat aaagaagagt taaaaaaaga
    27541 attatctgaa aaagctttaa aagagttaga atacataatc ccagcttcgg ctagaataat
    27601 agatgtaaaa cataacttta aggttaataa aaatatgtta gaatatttaa taacagttca
    27661 aactagtgaa aatattgcta aaatttatcc tcttagtaag tcagaagcag aaaggtttat
    27721 aaaggaagaa agtaaaccag atgaaggtga agaggaagtt ccttccaatc cagaaaaaag
    27781 accattaaat gatataagaa atgagtttga tgaggataat aaggataata aagatcaaaa
    27841 caattctgat gaaaataata gtaatcaaaa tagtaataat agtcaaaata ataatagcaa
    27901 taacaactaa taaaattaaa cttaaaatct taggaggact aattcaattg ataatacaaa
    27961 agaaatttac aatagcagat ggtaactttg aaagagagtt atttggcaac tttgatgaaa
    28021 atgtaaaatt aatagagaag actttaaata tagatgtaat acttagagaa ggcaatataa
    28081 ttttaattgg agaagaaaaa aatgtagatt ctgcattaaa acttatgaat gaattacacc
    28141 aaactgtatc aaatggtaag catttagata agcagagtat ttcttattca ttatctcttt
    28201 tgcttgaagg aagtgaacaa aaaataaaag aacttgaggg gacaatcgtc ataactcaaa
    28261 ggggaaaagc tgttcaacct aaaactttag gtcaaaaaga atatataaaa cttatagaaa
    28321 ataatgatat aacttttggt gttggacctg ctggaacagg aaagacttat cttgcggttg
    28381 ctatggcagt aaaagctttt aaaagagatg aagttagtag aattatactt acaagaccag
    28441 ctgtggaagc aggagagagt ctaggttttt taccaggaga tttaaaggac aaagttgacc
    28501 catatttaag accactatat gatgccttat ttgaaatgtt gggtgctgat aaatttaata
    28561 agtatttaga aagaggtact attgaggttg caccacttgc atttatgaga ggtagaacac
    28621 ttgataattc atttataata ttggatgaag ctcaaaatac tacttctgaa caaatgaaaa
    28681 tgtttttaac tagacttgga tttggctcta aagctgtagt tacaggagat gtaactcaaa
    28741 cagatttacc acaaaataag aagagtggtc taatacaagc tacagagatt cttaaaggtg
    28801 ttccaggtat tgggtctata atgctaacag atagagatgt tgtaagacat gagttggtac
    28861 aaagaataat aagagcttat gagaagcacg acaaaagaga agaatttaaa aaagaagaac
    28921 gtaaaaaagc gaaaatgcag gaaaagactt tcaaaagaaa gtaaaagtgt agtaatatag
    28981 atatatagca ttattaatga ataaaaagta ggtgtagtga atggatttga tactagatga
    29041 tagacaagat aaattagaag taagtgagga gttaattgaa aagataaagg atataatcat
    29101 agaatgttta gattatgaag gatatgatga taattatgaa gtgagtcttt cttttgtaga
    29161 taataaagag attcatgaat taaatagaga atatagagga gttgatagag tgactgatgt
    29221 tttatcattt cctcttttaa gtgatgattt tgaggatgtt gagttagaag aagaatccct
    29281 aggtgacata gtagtatctc ttgaaagagc cttagaacag agtatagagt ataaccatag
    29341 ctttgaaaga gaagtatgtt ttttaatatg tcatagtatg tttcatcttt taggttatga
    29401 ccacgataca gatgaaaata caaaagaaat gagagaaaaa gaagaacaca tattaaataa
    29461 gttaaacata acaagggagt agtttttatg aaaccagaaa aaactcgtca aggtataatt
    29521 aaagcattta atgcagctat agaagggata ttgtatacat ttaaatttga aagaaatatg
    29581 aaaatacatt atttaggttc agttgctgtt cttataataa gtttgttttt taacttttca
    29641 aaattagaaa tgataatgtt acttatgtcc atatgcttag ttgtagttgc tgaaatgttt
    29701 aatacagcta tagaaaaagc tgttgattta gtaactgatg aatatcatgt tcttgctaag
    29761 atagcaaaag atgttgcagc tggaggcgtt ctagtggcag cactaaattc agtagtagtt
    29821 ggatatattt tgttttatga taaattgaca gatatatctg gaatattaat ttataaaata
    29881 agagaatcag aattacatat aaccttgata tgtatattat tggttcttat agctgttgta
    29941 gttgtaaaag cattgacttc tacaggtact cctctaaaag gaggaatgcc aagtggtcat
    30001 gctgccttag catttgctat agcaactgca attacactta tgactgaaag agttgttgcc
    30061 tctactcttg catatataat ggctgtatta gttgcccaaa gtagaattga aggaaaaata
    30121 catacctttt gggagactat tgctggagct ttgttaggtg tgttgatagc tatattagta
    30181 tttcagcttg gaatgttcta taattaaaat aaatgtacat aaaatattta aaataaaaca
    30241 aatctgtgct aattaaattt ttatgatata atgtagagta ttaaataaaa ataactttta
    30301 attattgtat acaggaggag ttgttgtgga taacatagaa ttgttaaggt tggcagaaga
    30361 tgccagacaa cattcttatg ctccttactc aggttttaga gtaggtgcag ctcttttaac
    30421 taagagtggt aaagtttata ctggatgtaa tatagaatgt gcatcattag gtggaacaaa
    30481 ttgtgcagaa agaacagcta tttttaaagc aatatcagag ggtgataagg atatctataa
    30541 aatagctata gcaagtgata actcagaaaa taatgagcaa acatatccat gtggaatatg
    30601 caggcaggta attatagagt ttggttctga tataaaaata ataacgggat atactaaagg
    30661 agaggtgttt gagcatagta taaaggattt gttgccaaac tatttctctg gaaatgattt
    30721 aaaataaatt taaaagtata aataaattta agtaattaaa acaagaagat aaaaagttag
    30781 gagacagtta atgttcaaat caggttttgt cagtatagtg ggtagaccaa atgtaggaaa
    30841 atctacttta atgaataatg tagttggaga aaaaatagct ataatgagtg ataagcctca
    30901 aactacaaga aatacaatac aagcagtata tacagatgaa gaaatgcaga tagtattctt
    30961 agatacacca ggtattcaca aaccaaaaaa taaattaggt gaatttatgg taaaggcagc
    31021 aacagaagct tttaaaaatg tagatttaat attatttgta gttgatgatt ctaaaaaaat
    31081 aggtcctgga gatagaaaga taatagagga tttaagaagt gttaagacac caataatttt
    31141 agttgtaaac aaaatagacc aactagacca aaaagatgag ctatttgata taatcaaaat
    31201 gtatgataga gaaggcattt ttaaagaaat agtaccaata tcagcattaa aaggaaagaa
    31261 tacggatacc ttaataaaag ttatacaaaa ttatttagaa gaagggccaa agtattttcc
    31321 agattatatg attacagacc agccagaaag agtattaata gcagaactta taagagaaaa
    31381 agttcttcac tatcttaatg atgaaatacc tcatggtgta gctgttgaaa ttgaaaaaat
    31441 gaaagcgaga aatgataagg aaatagtaga tgtatctgct gtaatatact gtgaaagaga
    31501 ttcacataag ggaataataa taggaaaaaa tggtaggaaa ctaaaaggaa taggtaagtc
    31561 agcaagacaa gatatagaat tgcttttagg ttcacaaata aatcttcaat tatgggttaa
    31621 agtcaaagaa aattggagaa atttacaaaa ttatataaat aattttggat ataatgataa
    31681 ataaaggtgg gttatatgga taggaaactg gatgcttccc aagatttatt atatgaagta
    31741 aaatcattaa ttgataataa taaagtattg gagttaagag aattaataga ggaatatcat
    31801 ataattgata tattcgatat aatggaaaat ttagaggaag acatgaaaat tcaattattt
    31861 gaagtacttc ctttagatat ggcatcttct atattagaag aaggtagtgt agagtttttt
    31921 attagtattt tatctaaact ggatgtagaa cactcgaaaa atatacttga acttatgtct
    31981 cttggagata tggcagacaa gttgagtgaa cttgaagaag aagaaagaga acacataata
    32041 aatcttttga atcaagagaa tgccgattat gtaaaagagt tactattcta tgatgaagat
    32101 tctgctggtg gaacaatgac cactggatat atttctataa ataaagatat gacagctctt
    32161 gaggctatag accatatgag agaagaagct gaagaagctg aaactattta ttatatatat
    32221 gtggttgatg atgaagaaaa actagttgga gtattgtcat taagagaatt aataatagct
    32281 agagacgcaa atatagtaga agatttgatg agtgaaaata taatctctgt ttatgtagat
    32341 gaagatagag aagaagcagt aagattagta tctaaataca atttaatagc aattccagta
    32401 gtagacagac aagaaaaatt aaaaggaata ataacagtag atgatataat tgatgtaatg
    32461 gaagaagaag caactgaaga tatgtataaa tttgcaggaa gttcagaaca tgaaagagaa
    32521 gttgctgaaa aagaaaaccc tacattgaga gagcaaataa tatcagcact tagaggacga
    32581 ttaccttggt taattattac cttagttgga gggttgttgg cttcgttaat actttctaac
    32641 ttagactata taatgaatcc tgtatatgct ccattagtat tttttatacc tgtagtaatt
    32701 ggtatgggtg gaaatattgg aactcaatca tcatctgtta cagtaataac attatctaat
    32761 aaggatttaa attttagcaa tgtggttaga gaaggtattg taggtattat tacaggtctt
    32821 ttgtgtagta taataacagg tattgtaatt tactttgtta tgagaaagtt agacattgta
    32881 ttaattgttt caatatcatt atttataaat atggttttag gtgcaacaat aggtgcgttt
    32941 atgcctgttt tattaaaaaa aatggatgca gacccatcta cagtttcatc accaattata
    33001 tctacagctc ttgatataac tggtatagca gtttacttta taataacaac ggcattattg
    33061 tcaaaaattg tttaataact ttatgtatga tttatcctct tcttactaaa aatataagta
    33121 taaatgtaag aagggggttt tttaaaaatg aacaatatca gttgggaagt ttttaaaaaa
    33181 acaggtagta tagatgcata tttatatttc tgtgattgca aaaacataca tgaagaaatt
    33241 gaagaagtaa gggagaaaaa agaagacgat gataatcctg aacacccagg ggatagtact
    33301 taaagctata aggtataagg aaagtgatat aatacttaca ctatttacaa gaaagcttgg
    33361 aaaagtatct gcaatagcaa aaggagctaa aaaaaataag agctctttgc tttcatcatc
    33421 acagttattt tcttatagca attttacctt aaaaaaacaa ggtaatatgt acaaggttac
    33481 tcaaagtgaa ataataaaaa gtttttataa tatatcctat gatatagagg cattttctta
    33541 tgctacatat attacaaaat tagttgaaaa ttctatactt gaaaatcaga ctaataatag
    33601 gctatttata ttacttgcgc agactctgta tttgtataca caggataata cagacaatag
    33661 gtttataaca gcagcattcg agttaaaatt tttagactat ataggattta aacctatagt
    33721 aaataaatgc attaattgtg aatgtaaaat tttacaaaat tctgtgttta atatctacga
    33781 aggtggtata ctatgtagta agtgtagtaa actttttgat aataatataa aattagactt
    33841 aacaactata agtcttatgg aatatgtatt aagaaatgat attttaacat gtagtaaagc
    33901 taaagtatca aaatatataa cacatgaact tgagaatatt ctagacaagt atttgaaagt
    33961 ttatgttgat aatattaatt ttaaatcatt acacgtattg caaagtgtaa aaaataataa
    34021 gggagtggat aatgatgagt agtaatataa ctattgaaat ggttgattca gtctttgaaa
    34081 gagttccagg agcaacttat gcagaagcaa aagaagcatt agtaatgtgc aatggagatg
    34141 taattgaggc agtaatatat ttagaaaata aaaaaaatac ttgtaattgt aaagcaaaat
    34201 ctgctaaaga aactatggaa gaagtatttg gcaaagatgg agaaaatata aagtttcaat
    34261 taaaagagct tcttagaaaa agtagtgtag tgagaataat agttgaaaaa gatggaaaaa
    34321 ctatgatgaa tattccttta acagttggag ttgttggatt agcttttgta ccattagcta
    34381 ctttagttgg attatcagct gctgttataa gtaaatatcg tataaaagtt caaaatgaag
    34441 aagatggaga aattgtagat ttaggtgaat tgaatgaaga aaaacttcac gttttgaaga
    34501 atatgataac taatgcagca aaagatgtga aggatgtagt agttgacaaa aaagaagatg
    34561 acaaagatgt aactgctgac cttatgaaag aagaatctga aaagaataaa gaagacgaag
    34621 acgaaaataa atagatataa agtgactttt aaaaaatgaa gagaagataa ttataagaat
    34681 tatcactaac attgacaaat tttatagaat ttgatattct aaaagttgta gaagaactat
    34741 taaaatagcc tttcgtgtag acgagaggct ttattgttat aggagggatt tttatgaatt
    34801 ttcaagagat gatactggcg ttgcaaaaat actggtcaaa gcaaggttgt atcatgatgc
    34861 agccttatga tattgaaaaa ggtgcaggaa ctatgaatcc taatactttt ttaagatcat
    34921 taggacctga gccttggcaa gtatgctatg tagagccttc aagaagacca gcagatggaa
    34981 gatatggtga aaacccaaac agactatatc aacatcatca attccaagtt atactaaaac
    35041 cttctccaga taatatacaa gaattatatt tggaaagttt aaaagagatt ggaattgacc
    35101 caagtgagca tgacataaga tttgttgaag ataactggga agctgctaca gttggtgctt
    35161 ggggacttgg atgggaagtt tggctagatg gtatggaaat aactcaattt acgtattttc
    35221 aacaagtagg taatattgaa tgtgagcttg agacaggaga aattacatat ggattagaaa
    35281 gattagctat gtacattcaa gaagttgata gtgtttatga tttaaaatgg aatgataaaa
    35341 taacttatgg tgaagtattt aataaagctg agtatgaaaa ctcaatgtac gcatttgaat
    35401 tatgtgatgc agatatgttg tttaatctgt ttgatatata tgaaaaagaa gcattaagac
    35461 ttatggaaaa tggccttgta ataccttcat atgactatgt gttaaaatgc tctcatgcat
    35521 ttaatacatt ggatgcaaga ggtgcaatag gagttagtca aagagcttca tttataggta
    35581 gagttagaaa tatggcaaag actgttgcag aaacttttgt aaaacaaaga gaagaaatgg
    35641 gattccctct tttaaaggac ggtgacaaat aatgaaaaat tatcttttat ttgaaatcgg
    35701 tgttgaagaa atgccatcaa gatttgtagg cagtacacta gaacaattaa aaaacaatct
    35761 tagtaaatta tttgatgaaa atagaatagc ttttgataaa ataaatgcgt atgggacacc
    35821 aagaagactt actttagtag tcgaaggatt gagtgataga caaaatgatt tagaagagga
    35881 aataaagggt cctgctaaga aaattgctgt tgatgcagaa aataatttaa caaaaccagc
    35941 attaggattt ataaaaagca aaggattaag cgaatctgac atatatttta aacaagttgg
    36001 aaaagaagaa tatatatttg cgactataaa gcaagatgga aaagaaactt cagaagtact
    36061 aaaagacata ttaccagaga ctataaaatc tgtagtattc ccaaaagcta tgcgttgggg
    36121 aggaaaaaat cttaaatttg caagacctat aagatggata gttgcattgt taaatgataa
    36181 agtattagag tttgatttag aaggaataat atcttctaat gtaacaaaag gtcatagatt
    36241 tttaggtgaa agtacaatag aagttgattc tatagaagat tattttgaaa aattagaaaa
    36301 aaattatata gttttagacc aaaacaagag aaaagaaatg ataagaaaac aatgtataga
    36361 agtagcaaat tctttaggtg gagaagttga atttgatgaa gatttacttg aagaggtaac
    36421 ttatttagtt gaatatccaa ctgcttttta tggagaattt gatgtggatt atataaaact
    36481 accaaaagaa gttgttataa ctcctatgaa acaacatcaa agatattttc cagtacttaa
    36541 agaaggaaaa ctgttaccta actttatagc tgttagaaat ggtgattctt acagaataga
    36601 caatgttaaa gctggaaatg aaaaagtttt agaagcaaga cttgcagatg ctttattttt
    36661 ctatagagaa gatactaaga gaaacttaga aagctacata gaaaaattaa aaactgttgt
    36721 attccaagta aaactaggaa ctatttacga caaaacttta agacttgaaa aattaagtgc
    36781 tgatatactt gatagtttag gtttagtaga tgaaaaggca gatactataa gggctgctaa
    36841 attatctaag gcagatttag ttactggaat ggtaggagaa tttgatgaat tacaaggttt
    36901 tatgggaaaa gaatacgcaa aagttggagg agagaatgat gttgtaagtg aagctatttt
    36961 tgaacattat cttccaagat ttgcaggaga tatacttcct aagactaatg caggagttgc
    37021 cttatcaata gctgataagt tggattcaat agcaggtttc tttgctatag gaatacaacc
    37081 aacaggttct caagacccat atgctttaag aagacaagct ttaggtatac ttagtatact
    37141 tatggacaga aaagttgctg tggatataaa agtacttatt gaacatgctt tagacaatta
    37201 ttctgaatta gattttgata aagaagaagt tgttgagcaa ataatgaatt tcttcaatga
    37261 gagagtaaaa aatctattta aagatttagg aataagatat gatgttgttg atgctgtttt
    37321 aagttctgat ataaatgatg tttctgatat gcatatgaga gctttagagc ttaataattg
    37381 gctagaaaaa gatgaattag tggagatgct tactgcattt aatagagttt ctactctagc
    37441 tcaaaaagca gaaagtgata aaattgatga atctttatta aaagaagatg ctgaaattaa
    37501 actttacaat gaatttaaac aagtaaagat aaaagttaat gaattattaa aggataagaa
    37561 atatggtatt gctttagatg aatttgcatc tttaagacca gtagttgata atttatttga
    37621 tacagttatg gttatggata atgatgaagc tattaagaac aatagacttg ctttacttaa
    37681 acaagtatat gatactatgc tagaaatttg tgatttatct aaaattgttt ataaataaaa
    37741 tttaaactat ttataagaaa taaggctgtc tcaaaataga gatagtctta tttttcattg
    37801 ttgagatgat gaaaatatat ttgatttcat agaaaaagag tgtctcataa actaaaaagt
    37861 tcattttgag acacccatgt tttttgtaag taaatataat aaactatatt ttatacttat
    37921 cagttatttt gtttaaaata ttatcatttg aatttaccca ttcataaaaa ttgcctgatg
    37981 atgcgtccga tattttataa agcatttcag gaatatcact agctttaaaa tctccaagtg
    38041 attttcccaa tctagtctta cctatttcaa aactgcctcc aacaaataat tcaaatgtat
    38101 ctgtagatat accatcaaca ttcttctttt taccacataa acctatgcta ccaacttgat
    38161 gaacaccaca tgaatttaaa catcctgata tatataactt aggtgcttta tttaaaattt
    38221 cttcattatt ttgtaatcta aaataatcta tgatttcatg taacattttc tgactatttt
    38281 gtatgcccat ttgacaaatt ggaacaccta tgcaagatat actttgttct aattgagaat
    38341 tacaactaat agttttactt atttctaata ttctttttgc ttcatttcca tttaaattta
    38401 aaatatataa tccctctgtc attcctagtc taatcatagg attttcaaca ttatctaatt
    38461 ctttaagtaa agtacttaaa tcctttgtta acaaaatacc tccaactgga tgtatatata
    38521 cagtgtataa accatcctgt ttttgaggta ttagtctaga atcttgtata tctattttaa
    38581 ctccaggttt agagtaatct atttcttcaa tattgagttc aagactacca ttttcttttt
    38641 ctaatttata gtattcttta aatttttcta agaatagttc ttcaccaagt gattcgacca
    38701 tatatctaac cctagctctg tttttatttt cataatttcc ataatccata aagaatttaa
    38761 tcataccttc aacacaatat aaggcatctt tcggctttat aagttcatca agttctaaag
    38821 caactttggg ttgtttacct agacctccac ctaagaaaac tctgaaatat ggttcatcat
    38881 ttttcaaagt agcaacaaat cctaagtctt gtatactaca atgagcagta tctgtgtaac
    38941 aattagaata agaaactttt aattttctag gtaaatggta tgtagtagca tttttgataa
    39001 aatatttatc agtagcaact gcatatggag taacatcaaa gacttcacta gggtcaactc
    39061 cagataatgg agataatccg acatttctag gataattacc accaccacct ctagtgaata
    39121 tatcattttt tattccttct tccatgatat caacaattga atttatagat aaatcgtgca
    39181 attgtatagc ttgtctagtt gttaagtgta tcttatctaa gttgtgttta atagctattt
    39241 catagatttt attaagttga tactgagata aaactccagc agatactcta agtcttatca
    39301 taaaagattt tttgtctctt tgagcataaa caccatatcc accagaaaat cccttatatt
    39361 gcattttatt tatttctcca tttaaaaatt ttgaagaata ttctttaaaa ttaggtagtt
    39421 catttaacaa tatcttttta aaattatcca ttatttcacc tcattgttag tcttgtataa
    39481 aaatatatct attgtataat agatatatat attattgtct agtcaggata aaaatataac
    39541 agaataattc tattaaaagt aagaataata aggacatagt gagtattttg tttttatttt
    39601 acaaattaag tataatattt ttgttttata aaatataatt aagataaata gataattttg
    39661 taattttaat atataatatt tagtagagtg tgggagtagc acttaatatt tgcaggagga
    39721 gtttttgtat atgggagaaa taataaatgc attagataga gccctagaca taattttgct
    39781 gttatatcat gaaaagagag aaatgggaat aactgaaata agtaaggcta tgggagtata
    39841 taaaagtact gtacatagaa ctttggttac tcttgaaaac aaaggttttg ttatacaaaa
    39901 tgcagagaat ggtaagtatt ggctaggaat aaatttatat gcaataggta tggttgtggg
    39961 agaaaaaatg tcattgactg aaatagtaaa accatatacg aaaaaattaa atcaagaatt
    40021 taacgaagta gttaatgtat ctatattaga agaaagagct caagattctc caagaagtat
    40081 cataatacat aaagagtatg gttcaaatca attactttca gtaaatccat ccgttggttc
    40141 aagtagtgaa tgttattgtt cagcagttgg taaatgtctt atggcattta atgattctat
    40201 agattttgaa aaatatagaa aaacaccaat acacaagtat acagaacata caattgataa
    40261 ttgggatgat atgatgcttt ttttagcaaa aataaaagaa caaggatatg ctatagatga
    40321 tgaagaacta gaacatggac taacttgtat tggagcacct atcttagata aaaataataa
    40381 agctattgca gccataagtt tgtcaggtcc aactataaga atgagagaag gggactttga
    40441 gtataagatt aaaagagtta tagaaactgc aaaaagtata tctgaacttt ttagatgatt
    40501 tttaatctcg ataataaaaa taggctatat ttaaattgtt atttgtaaat atcagtttaa
    40561 atgtagccta tttttcttta atctaaaatt aaatattctt aaaatttaaa atgttattga
    40621 aatatataag cgaatgtgtt atcttttaat taagcaataa aaatagaaca aggttctata
    40681 ctatagaaca aaattgatgc atatatagta tttatacaaa atataaatat tatatattta
    40741 aaattaagta aaaatattac ataaagggga gatttgagaa atggaaaaag tcaagttgag
    40801 tgttgaaaga tgtaagggat gctatctttg tattgaagct tgtaaaaaag atgcaataag
    40861 tctttctgac tacacaaata aaaaaggctt tgtaactatt aaagtcgacg aagaaaaatg
    40921 tattcaatgt ggcgcttgtt atacaatgtg ccctgattta gtatttgaga tattataaat
    40981 tatttttatt agaggggaga aaaggtatgc aaagtttatt aattaaagga aatgaagctt
    41041 tagcagaagg tgctatacgt ggtggctgca gattttatgc aggttatcca ataacaccac
    41101 aatcagaaat attagaaatg atggcatgga gacaaaaaga agttggagga gtatttatcc
    41161 aaggtgaatc agaaatagcc agtgtaaata tggttatggg agcaagtgca cttggagcaa
    41221 gagctatgac ttcttcatct ggtccaggat tttcattaaa acaagaaggt attgcatatt
    41281 gggtatcagc tggtttgcca gcagttgtag catgtgtgca aagatatggt ataggtgatg
    41341 gatttatagg agctggtcaa gataattatt ggcaagcagt aaaaggcggt ggaaatggag
    41401 attatcattt aatcgtgtta gcacctaata gtgttcaaga aaatatggat atggcatatg
    41461 aatcttttga attggctgaa aaatatatgc atccagtact tatactttct gatgctacaa
    41521 taggtcaaat ggtagaacct tgtatagcac caccaatgaa agaatacgat atggataaag
    41581 ctccatgggc tgcaaaagga acacctgtag gagaaaaaaa taaagtaata actgatataa
    41641 catattttga agaccaagat acatggcaaa aagggtatat agaaaaaatg agaaaagttc
    41701 aagaagatga acaaagatgg gaagagatag aaatagaaga tgctgaagtg gtatttgtgg
    41761 catacggtat ttcatcaaga atagcagaag gtgcagttag agatgctaga gcaaaaggaa
    41821 tgaaattagg tctaataaga ccaaaaacat tgtggcctta cccaagaaaa gcatttaaga
    41881 aattaagtga ttcattgaag ggacttgtaa cagttgaaat gaacatgatg gcacagatga
    41941 aggaagatgt aattatagca tgtgataata gatttccagt atattcagta gcaacagctc
    42001 aatatatggc agaagtaaat caattaatag attttgctca acagattata gatggaaaag
    42061 ctaagcaaga ggaggtattt taattatggc taaaaaagca ccagcaataa tgatgtgtga
    42121 aaatatgttc tgtggaggct gtggacactc tatagtcgat agaattattg gagaagtatt
    42181 aacagaaatg gatatatgtc aagatacaat tatatgttca gatataggtt gtaatcatat
    42241 gtttcaattt agtatgaatg tagatgttgt cgttccacct catggtaaaa tgggagcagc
    42301 aatggcagct atgaaaagag ttagaccaga taaatatata tttacaatag ctggagatgg
    42361 tggagcttat gcaatagggc ttggagaaac tatgtcagct gctactagaa atgataatgt
    42421 aatatttctt gttatgaaca ataatatgtt tgcaatgaca ggagggcaaa tggctccaac
    42481 tactatagaa ggtcaatata cagcttctac tccagcgggt agaaattacg cagatcatgg
    42541 ttcaacattt gatgttgtaa aagttatggg aaatttagat atagcatatc tagcaagagg
    42601 aacaataact aaccctaaag aaataaatac aacaaaaaga tacatcaaaa aagctctaga
    42661 aaaacaaaaa gaaaagaaag gtttttgctt aatagaagtg cttgtaccat gtccaacaaa
    42721 ctggggatta aagccagtag atgctatgaa gagaattgaa aatgaacttg ttccaaagta
    42781 tccacttgga gaatttattg attagggagg aaataatatg ttgaatgaaa ttatatgtgc
    42841 tggttttgga ggacagggag ttcttacagc aggaaaaatt ataatgtatg ttgcatataa
    42901 ggcagaaaag aaaattacat ggttcccatc ttatggaaat gagatgaggg gaggaagtgc
    42961 aaattgcaat gttgttgtaa gtgatgaaaa aattgcaaat ccttgtgtaa aaaatccaga
    43021 tatactattg ggaatgaaca gcgtatcagt agataagtat gaaagtttaa tgaaaaaaaa
    43081 tggttatatg tttgtcaatg aatcactagt aagtaaggat agaaagtaca gagaagacat
    43141 taatgttata aaagtacctg ttactgaaat agcacaaaat ttaggcaatg aaaaagctgc
    43201 taacatttgc atgttaggtg ctatggtaaa gagtttggat ttatttacaa aagaagaatt
    43261 tgaaagtggt atgtgtgaat actttgaaaa tcaaggcaaa ggtaaattca attctaaaaa
    43321 tgtagaagca tttgaagctg gatacgacta cgaatgaata ttttaaatat ttaaaataat
    43381 tattgaaata cttattttta tatgttagta tttaattaat aaaatataga acgtcgttct
    43441 ggacaatggg acaaagtctt taggtcggaa tacaagaacg acgttctgca tagtagaact
    43501 agggggtgtt aaattgaaag ttttagtaat aaatcctggt ggtacatcca caaaaatttc
    43561 tgttttccaa gatgaaaatg aaattttgaa aaaaaatata acccatacaa gagaagattt
    43621 aaaaaatttt tctaaagtat ttgatgaata tgattataga aaacaactga tagtagatat
    43681 tttaagtagt gagaatcata gtataaattc ttttgacgca gtagttggtc gtggtggact
    43741 tatgaaggct ataaaaggtg gtacctatac tgtgagtgaa gaaatgattg aggatatgag
    43801 aaatgaaata aatggggaac atgcatcaaa tttaggtgca ttattagcta aaactatagc
    43861 tgatgaaata ggagtacaat catttgtagt agacccagtt tctgtagatg aatttgatga
    43921 tgtatcgagg attacaggaa tatcagacat agaaaaagca agttggttac atgcattaaa
    43981 ccataaagct gtttcaagaa aaattgcaca aaaactaggg ggaaaatacg aggattataa
    44041 ctttattgta gctcatttag gttcaggaat atcgattgta gcacataaac aaggcaaaat
    44101 gattgatgga agtggtggta ggtctgatgg tccattttct ccagaaagga gtggtggatt
    44161 acttgcatat cctcttatag aattgtgtta ctccggaaaa tatactcatg atgaaatggt
    44221 cgcaaaagta agttcgattg ggggaatgta cgattattta ggaacaaagg acatgataga
    44281 aattgaaaac agaatcaagg atggtgatga aaaggcaaaa cttataatgg atgcatttat
    44341 ttatcaagta gcaaaagaaa tttctatgta tggagcttca ctttcaggga atgtagatag
    44401 agttatcttg acaggaggga tatctcactc agaaatggta gtcaaaggag tagcagaaaa
    44461 agtttcatat ctagctccaa tagagatagt accaggagag atggaaatgg aggcacttgc
    44521 tctaggggca cttagagtac ttaatggaga agaaatagca aaaacttatt aatatttagg
    44581 agaaagagta tgttaaaaga ttttaataat ttattagaag aggctaaaaa gcttgaacct
    44641 gttattgtaa gtgttgctgt tgcagaagac aaagaagtat taaaggctgt gaaatcagca
    44701 acagatttaa acattataaa gccaattctt gtaggagaca aggataaaat agaaagtata
    44761 tcaagagaaa ttggattaaa aggtcatgaa ataataggtt gtcaaaatcc cgatgaatca
    44821 gttaaaaaag ctgtagagat agttaaaaat aaggaagcta aagttcttat gaagggatta
    44881 gtaaatacaa gtgtctatat gagagctata ttaaataaag aaacgggtct tagaacagga
    44941 agattattaa gtttgcttgc agtatatgaa ttacctcaat atcataaact tctttattgt
    45001 actgatagtg gaataaatgt atcgccaaac ttaaatcaaa aaaaagatat aatgacaaat
    45061 gcacttctta ctatgaaaag tataggcatg gaaaatccta aaactgcgat acttactgca
    45121 aatgaaatgg tagaccctaa gatacaatct acagtggatg ccaacctact tgtagagatg
    45181 gaaaaatcgg gagaaatacc aaagtgtata gtggaaggtc caatagcatt tgatgtagca
    45241 tttgatgcac atgcagcaga acataaaggt attaaaagta atattgctgg tgatgttgat
    45301 ttattagtat ttccaaatat agaagcaggg aatatattag gaaagtcatg gcttagattt
    45361 aataaagcta agtgggcagg tatagtttta ggagcaacaa atccgataat tttaggttca
    45421 cgttctgaca cagctgaaat aaaaataaat tcaatagcat tagcgtgcct agcatcacaa
    45481 taagaaatta ttattaagga ggaaaaagta ttatgaaata tgcaaaaaga atggatatgg
    45541 taaaagcttc agctataaga gattctcaaa agaaaatagc tcagaaggta gcaagtggtg
    45601 gaactgttat atcttttgct gcagggcttc ctgacccaaa tttatttcca atagaagaga
    45661 taagtgaaat aactcaaaaa gtacttaatg aaaggggaaa atttgcccta gcatatggac
    45721 ctactaaagg tgaagatgag ttgttagatc tattagtaaa aagaatgaaa gaagaagaaa
    45781 atattgatac aaaagcagaa aatattataa taactactgg gtcacaacaa gggatagcat
    45841 taagtgctat gattttatta gaaaagggag atgtagtagt tacagaaaat ccaagttact
    45901 taggtgcaat aaatgcattt agaccttatg aatgtgattt tttaggagta gatacagatg
    45961 aagaaggaat ggtaacagat gatttagaca aattattatc tgaaaattct aacataaagg
    46021 tgatatatgt aataccaaca tttcaaaacc ctacaggaaa aacatggtct ttacaaagaa
    46081 gaaaagattt tatggaagtc gtgaacaaat atgatgtcat agtaatagag gataatcctt
    46141 atggagaaat aagatttaca ccagaacctc tacctacact taaatctctt gatactaaag
    46201 ataaagtatt gtaccttggg tcattttcaa aggtattatg tccaggattt agagttgctt
    46261 ggatgtgtgg taatgtagac atcatagata aggcagaact tttaaaacaa ggtatagatt
    46321 tacagtcaaa tcaattttcg caagtacaag taatagaatt tttaaagaaa tacaacttaa
    46381 atgaacatat agaaaaaata agagcagagt ataagaaaag atgtctgcta atgttagatg
    46441 ctatgaagaa acatttccca gaggaagcta aatatacaga accagatgga ggaatgttta
    46501 tatgggttga acttcctgaa agtataaatg tagatgattt attagataaa gccattgatg
    46561 ctggagttgc ttatgtttct ggagagtcct tctttgcaaa taatggtcca aaaaatacta
    46621 tgagacttaa ctatacaaca atgtcagaag aacaaatcgt taaaggtgtt gagatacttg
    46681 cagaagttat aaaaaaagaa ctcaattatg cttgtgaata aatataaaaa gtgagggttt
    46741 aaatatggga gaaaaagaaa acggtataaa gtttggaaag ttaatgtttc aacttgttat
    46801 tatagcaata tcttgggaat tagtttatat tataccattt atccaatata cattatatga
    46861 tccaatccta aaagcattag aatgtacaaa cactcaacta ggttttctac ttacaattta
    46921 tgggttaggt aatatatttg gagctcctat aggaggctgg ttagcagata gatttgatta
    46981 tagaaagata attttaggtt ctgtatttct taatggagta gtatcatttt tatttgcatt
    47041 taatatgaac tatccatttg cagttgctac ttgggtaggt tgtgcaatca catcattagt
    47101 tatgaactat ccttctatgg taaaaatact tagagttata ggaaaagata atcaaggtaa
    47161 ggtttatgga tttaatgaag ctatggttgg agtttctgga gtagtaatgg gagcaatatt
    47221 cttatatatt tatacatgct ttgcaactcc tacattgggt atgagatggg taatgatatc
    47281 tttaggaata ttatcaatag caatgtgtcc agtattatgg tttgtaatta aagatgtaga
    47341 tattaaagaa gaaagagaag aagcaggtga aaaaatgtct gctggtgatt ttataacagt
    47401 actaaaatca ccaaatactt ggctagtagg aataagtata ttctgtgtat attcattcac
    47461 agttacaatg tcttatttta caccatatat aacatctgta cttggaggtt ctgtagcatt
    47521 atctggtgca ttagcaataa taagacagca tggtctaaaa ttatttggag caccatttgg
    47581 aggatattgt gcagacaaat taaaatctcc tacaaaagta cttttaccaa tatatgtatt
    47641 tggtatagca gtaatagttt tattcctagt attacctgca tcaactccaa tgactatatt
    47701 catagcttta acatttgtag taggtatatt aggttacatg ggaaaaggta tatattatgc
    47761 agtacaagat gaagtaaaag ttccagttaa atattcagca actactatag gtatagctgc
    47821 agctttagga ttttcaccag atgtattcca atttgcatta ataggacact ggatagatac
    47881 ttatggcaat aaaggttaca catatacatt tatattccaa atagcaattc tagttattgg
    47941 aatactatct tgtttatata tattaaaggt taaaaagaga agagcaagta aattagaagc
    48001 ttaaatattc aatttggtaa ttaagtatat ataaatacaa tacttatttt gatgaatgtg
    48061 attgtataaa taacataaaa aatatcaata gattaagttt tataataatg tttagagaat
    48121 aaagtataaa aatcaaaatg ttatcaagtt ttctaggttt taacataaag ttacccctga
    48181 aggtgtaaaa ttgagtggca attattatct attttcttac ctacataaac ctatttaatt
    48241 taaattctaa gagattttca aaatagatgg tagttgtact caattttaaa cctcataata
    48301 aatacgaaaa tttaaaa
//
LOCUS       DAGSRM010000024        46086 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.024, whole genome shotgun sequence.
ACCESSION   DAGSRM010000024 DAGSRM010000000
VERSION     DAGSRM010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 46086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46086
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.024"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            130..3678
                     /locus_tag="MJE33_001449"
     CDS             130..3678
                     /locus_tag="MJE33_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBY9647390.1"
                     /translation="MMNRLKPRILGISILFIIIIILTCGAVFKFKFKLDNKLKETTHT
                     YLSEFTEHNIINLKTRLSGQFDMLESIANFIGNLNNINDEMVIDLMDSDVKRSSLIRM
                     TISTLDGECYSNDGITHNVQDRDYFKKAKNGEKNISEPLKSIVNNKEIIVLSTPVYKS
                     GKIVGVLSGIYDSKKLNELLGLSAFDEKADVFVAKKNGDIVAQKTNKNRKTLSCIFQL
                     FDKDNCDEDDCLKTIKENMKNDKSGYFNYNLDNEKFMLNYQPLGVNDWYVFSVVPNKI
                     VSIQSENISYDVYIFALEILLVILIFIIYIACIINNNTKIIINEKEKFKVLTDNILGG
                     VKTCLKDEYMTMTYVSQGFLELTGYSCEDLNKIFKNRFKDMIYKDDLDKFNCIILNQL
                     EKQDKIEVEYRIVRKDGSIIWILERSKSINDNKGSECIQGVLTDITELKKIQEELDSK
                     RFEIQAINDSIVGGIVITEINDDFDSIYLNEGYLNMIGYTRKQLEDELNNKLIKLIFK
                     DDRTELINSINKQLKLSDTFASEQRVEKRDGTIIWILLKGRKMIYDNGKSVLCCVIVD
                     ITSAKLLEQELKKSEERHEIILKQTQNIIIEWDIEKDKMNISPNWYNKFKYDPITENA
                     TKNIIKSVDIYHDDMDKFISLIKLTSQGSEYEECEIRLRTRDTIYAWFSIKATTLFDN
                     NGKPYKVVGSLTDIDESKREAEKLKERAEKDPLTDLYNKKASQSLIEEYITLDKKRKG
                     ALMIIDIDDFKGINDNLGHLYGDAVLSEIANDLINLFRATDIVGRIGGDEFIVFIKDI
                     SETRDIINKAEELVKTFERSFLGKGRNYKISLSVGIAKFPQDGMDYMTLFRNADIALY
                     SAKGKGKNCYTLYDKKFDKVQYITNTRNAGDSDNVEMSFKEHITNYIFDILYESSDIE
                     VAVNLILSIVGKHFNVSRMYIFENSDDNRVCKNTFEWCNEGVTSEINNLKNICYKDLG
                     NYLLNFNADGLFYCNDIENLGSDVKKVLKPQGIRSILQCKILEGNEFRGFIGFDECSY
                     NRMWTNDEVNTLTFISKTLSMFLLKMRIQSKLEESLTIQKSVMDNMDIWAYVVDRNTY
                     ELLFINKKMLESAPNTHVGDLCYKAIWNNQDKPCKKCPMIYLKDGDDTYKSFMHNKKF
                     EVQANITTTTLKWFDGKDACLIFGSEITSIEAEENT"
     gene            3863..5797
                     /locus_tag="MJE33_001450"
     CDS             3863..5797
                     /locus_tag="MJE33_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HBY9647391.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLESAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHREKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLSREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACKKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMADKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFAINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKNEKPYFNRNNSKKNVSKGTNKNKKTN
                     TNNRSNKNQQKSSTKKKKKH"
     gene            5903..6898
                     /locus_tag="MJE33_001451"
     CDS             5903..6898
                     /locus_tag="MJE33_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBY9647392.1"
                     /translation="MKKYISIILLVVLTMVVLVGCSPGKDNPKNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKEGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMAAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     NYSDIKFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPEELGAYSEKIKIST
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            6926..7654
                     /locus_tag="MJE33_001452"
     CDS             6926..7654
                     /locus_tag="MJE33_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBY9647393.1"
                     /translation="MEVTLSCVILLFLWQIIALKIDNDIFLPTVGQVFNSIKEIMLNS
                     SFYIDILYSMGRCFFSFLLAMIFAIILSIVSYLNRFFRNLLKPINALTRSIPTMILVV
                     LALIWFEKDSTPFIVGFTIVFPILYDNVLGAILNIDKNLLEMANVYKIRFIDKVLKIY
                     LPAIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTMIQTEKINFNTSGIFAWIII
                     VLLISAILQIAQKFLARRAFLWKR"
     gene            7642..8340
                     /locus_tag="MJE33_001453"
     CDS             7642..8340
                     /locus_tag="MJE33_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9647394.1"
                     /translation="MEKIKLSIENINKRYDSRIIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNRVGNKLEASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTQLNKICDEYLELVGISDYKKFYPQMLSGGIRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFRNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSKELEKNEVLKLI"
     gene            8463..8936
                     /gene="ybaK"
                     /locus_tag="MJE33_001454"
     CDS             8463..8936
                     /gene="ybaK"
                     /locus_tag="MJE33_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBY9647395.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKLTGYIRGGC
                     SPIGMKKLYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            9133..9972
                     /locus_tag="MJE33_001455"
     CDS             9133..9972
                     /locus_tag="MJE33_001455"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HBY9647396.1"
                     /translation="MRKIKSEQIVEQVKKLCIEASLYLGEDVLSCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEIGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTAINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKK
                     VVI"
     gene            9985..10533
                     /locus_tag="MJE33_001456"
     CDS             9985..10533
                     /locus_tag="MJE33_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HBY9647397.1"
                     /translation="MIKITTPVNEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            10599..11780
                     /locus_tag="MJE33_001457"
     CDS             10599..11780
                     /locus_tag="MJE33_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HBY9647398.1"
                     /translation="MSKDYAKLALEMHEINKGKVSVESKVEVKTKDDLSTAYTPGVAE
                     PCLKIHENQDDVYRYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFAD
                     VDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHD
                     DQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCD
                     RSGALEASIENLNYVQKEMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVES
                     MSEKPIILAMANPTPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVK
                     AKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNR
                     I"
     gene            12075..12461
                     /locus_tag="MJE33_001458"
     CDS             12075..12461
                     /locus_tag="MJE33_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY9647399.1"
                     /translation="MEYKVNDQELNASMFIPFVNRIWPGDYDMDKTQTALSKTLNITA
                     YDDKVLVGCLRVLSDGYYFGTITELLVLPEYQKQGIGSKLFQLAKENTPTMLYFGAQP
                     GVEEFYEKNGCQRSLQSYIIKKKDSY"
     gene            12611..12874
                     /locus_tag="MJE33_001459"
     CDS             12611..12874
                     /locus_tag="MJE33_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBY9647400.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWK"
     gene            complement(13003..13845)
                     /locus_tag="MJE33_001460"
     CDS             complement(13003..13845)
                     /locus_tag="MJE33_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBY9647401.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVAHLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKTGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
     gene            14140..14874
                     /locus_tag="MJE33_001461"
     CDS             14140..14874
                     /locus_tag="MJE33_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY9647402.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGSPIEYTISIFRTDKYQYEIVLSE"
     gene            14884..16017
                     /gene="nagA"
                     /locus_tag="MJE33_001462"
     CDS             14884..16017
                     /gene="nagA"
                     /locus_tag="MJE33_001462"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBY9647403.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAQVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIDFDFTREIKRCTDIVLSIGHSNANYDQAKEAINLGVTNVTHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GNYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLDNTNLKVNEAINLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTLCLGDVVYKNI"
     gene            16116..16865
                     /gene="nagB"
                     /locus_tag="MJE33_001463"
     CDS             16116..16865
                     /gene="nagB"
                     /locus_tag="MJE33_001463"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBY9647404.1"
                     /translation="MRVLVCKDYDGMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKPENTHLP
                     NGMADDIEKECMNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDTKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYNTVYGDITPEV
                     PGSILQLHKDTIVIVDEAAASKLNPKDYKLV"
     gene            17040..17312
                     /locus_tag="MJE33_001464"
     CDS             17040..17312
                     /locus_tag="MJE33_001464"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647405.1"
                     /translation="MLKIIMFSGILSSIIKLPIIGILPTGNIKKNTGISDSKKDGKQR
                     RSNLGISIYQLPSFLYSLINNSVPSNKPIPPEEIKLIKMYLLKSCI"
     gene            17972..18676
                     /locus_tag="MJE33_001465"
     CDS             17972..18676
                     /locus_tag="MJE33_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY9647406.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNSIDMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAVSRDELLNKIWGYDIEVETRAADDTVKRLRKKILNTNI
                     LIETVWGFGFRLKEKS"
     gene            18678..20165
                     /locus_tag="MJE33_001466"
     CDS             18678..20165
                     /locus_tag="MJE33_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY9647407.1"
                     /translation="MKNGNDKNRIGKIKSKFKLKFSKKNISIKTRILLSILCLIIIVF
                     MIILVSFNVFLDTYIKTTANEELTKSTEIVEHMDSNFRPIKPPQDDGKLPPKVLSNFM
                     RNVQDKVRMAETQSDADAMVVDSKYNLIFPTREDDFLKNVDEMELIRECIQNEKLDLN
                     SDENTRISTRNKDYYISTVKITSIYGEQDKYLILFIDISKTLNLAQKINIVLISVMCF
                     AGILAIFTAVILSEKIAKPIKELCEFAKKMGQGDFKRTYFDFSDKELVELSTVMNKSA
                     EYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKHASDIILEESDRLSD
                     MVEDLLYISKIDNITKDFELVECDLREVLSNCGARQNVRAINKGIKFIYEFDEEMVLF
                     ECDEKNISKAFMNLIENALRYAKSEIKIVCKYNQKNIVVIIEDDGIGIKKEDLPHVFE
                     RFYKGVGGNHGIGLSIVKSIVNKHGGRIYVENGKKGAKFTILFKL"
     gene            20370..21983
                     /locus_tag="MJE33_001467"
     CDS             20370..21983
                     /locus_tag="MJE33_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBY9647408.1"
                     /translation="MKEQLIFNITNLVTVVFEAFVIHMFLSDFLGQKEGYIKVVRYAK
                     LGFIICLGFCNLITLNPKITMPLIFILIFSTSFLYKGNLKTRLFTTVLLSIFFILSEI
                     VVTSIFVLFVKEGFEIMLENNSIRVLATILSKIVFLLTCKIICLFKKDVHLDMPIKYW
                     LPLFLIPIFSLFLSVSIFDVSKFFSLESLKFLSLISSVGILYINFIVFYLFKFIIDKT
                     KLSMKYELLEKEIIHKEELRLSNECYKIIVEQTDSVVFEWNIKENKSFVSQAWTEKFG
                     YNNACKNIFKEIKDKDLVHSEDKAIFEGFLESIKKKNMHNQAVYRLKKSNGEYIWCRT
                     SITSIYNDENELLRVVGVIVDVDSDIKKYEELRTRAESDSLTNIYNKGTFEKLVEETI
                     VMNTGDKKDALFIIDLDDFKEINDNFGHPFGDFVLKTFADKIQTSFGSKDLVGRIGGD
                     EFVVYMQDYVTEVNLHKKAKELNRVLSDNYTDLSFSFDASVSIGIARYPQDGTSFFEL
                     FKNADRALYSIKASGKNSYCLFEEELYVQ"
     gene            complement(22346..23176)
                     /locus_tag="MJE33_001468"
     CDS             complement(22346..23176)
                     /locus_tag="MJE33_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBY9647409.1"
                     /translation="MSQVSKNYFTTGEFAKICGINKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLREVKMPLKEIKAYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKSACNIDANTITLKEHEEEYLVKTPVTYKEQFLGDEEENFLYECI
                     NFMDNYELSDYGTIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIKPKGLYVTA
                     YHKGSYETIYKAYEKLLNFFNQNNLRIGDFVYEEYLLYDISVRDSNEYLTQISAEVKM
                     "
     gene            23249..24976
                     /locus_tag="MJE33_001469"
     CDS             23249..24976
                     /locus_tag="MJE33_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9647410.1"
                     /translation="MKKLIHFLKPYRVLIVVVLIFTFLQTLGTLYIPTLTANIVNNGV
                     VKGDIDYIVKTGLMMMIVAGITALSAVLVCKVSANLSSGFCRDIREAVFIKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKAMTIIIV
                     VVMFLFMLSAFLVGKKLIKLFKMMQIKMDNMNRVLREVVTGVRVIRAFNRSRFEKKRF
                     DRTAIDYSETAISINKIFAVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALV
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIVDGNGHKDTVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISI
                     NGQDIKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEVKHAASIAQADE
                     FIYDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSFSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            25013..26875
                     /locus_tag="MJE33_001470"
     CDS             25013..26875
                     /locus_tag="MJE33_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9647411.1"
                     /translation="MSEKRIDEQDIEVLSFEDGMSAEKAKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLISAVFIYIQQYIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASRKAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPKGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKSDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGSHKELIEKKGFYEELYNSQFTSEYDEDVV"
     gene            27208..27897
                     /locus_tag="MJE33_001471"
     CDS             27208..27897
                     /locus_tag="MJE33_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HBY9647412.1"
                     /translation="MNKKLNYRFEMKHKITEADVLALKSRLYPIMKKDENASKDGKYL
                     IRSLYFDTPEDKALLDKLNGVAIREKFRIRFYNNDCSYIRLEKKIKHYNMTSKLSTNL
                     TKKEVINILNNDIEFLKESTNCLLREFYLKLKCERLEAKSIVDYNREAFIYPVGNVRV
                     TIDSDIKTSVNSVDLFNKDLPTVSVIDENMTVLEVKYDEFIPDFIKDLIQINKTTSTA
                     VSKYASSRLYI"
     gene            27993..28679
                     /locus_tag="MJE33_001472"
     CDS             27993..28679
                     /locus_tag="MJE33_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HBY9647413.1"
                     /translation="MILIMTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMV
                     YKKTYMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDP
                     MDLVFLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNED
                     SEDIATDKIKKVFTKYQIKSKSVTPEKGIELVFEVRMKDGETSLINDLSQVDGVTNAV
                     LVSYNGDYVA"
     gene            28696..30603
                     /locus_tag="MJE33_001473"
     CDS             28696..30603
                     /locus_tag="MJE33_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HBY9647414.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGVYSTSSNKSVDLYSDVYIEKYF
                     NRDKVMEVNIEIDESDLKDMNENAIKEEFKVAKVTVDGDTYGNVGIRTKGNSSLISVA
                     NSDSDRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLATPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGNVTGDLYKSDEGSSLQYKGDDPESYS
                     NLIVESDKKTADWSKITKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISSLLK
                     NETYKTKYHKYLEEIVTKYLDSDYLENMTTKLHDMIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKGFDNNNSNNSDSNNNSNSENKHSGNQSDEKEVNAELTSSVVKA
                     NTDNENKNKTTNDSESKNNTDKDKSGNDNNQKLEGPMGKGGKSIPGVLEVAEDMSKTI
                     KSQLSGETSSTKQNSGDESSSGIKGSEKFDEDMSGMPEPPEGMDGKMPPGMGNMDKGD
                     MNGKNGNMNMDRNQDNPREAGGFGNRGGGSVSKTTTYFKLILGGASMIIMSIMLVGVS
                     RVKRRRFIKSK"
     gene            31055..31744
                     /locus_tag="MJE33_001474"
     CDS             31055..31744
                     /locus_tag="MJE33_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647415.1"
                     /translation="MLQKVLWKFLKLVVGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADN
                     LFVGIIMLILGLVLLSVGMVFYMGSGLGSGPRDGLMVAIQKKTGKSLKLIRGTIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAAVQNIVK"
     gene            32180..32719
                     /locus_tag="MJE33_001475"
     CDS             32180..32719
                     /locus_tag="MJE33_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY9647416.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            32738..33781
                     /locus_tag="MJE33_001476"
     CDS             32738..33781
                     /locus_tag="MJE33_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9647417.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKENN"
     gene            33783..34619
                     /locus_tag="MJE33_001477"
     CDS             33783..34619
                     /locus_tag="MJE33_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9647418.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYMKVFGRSILLAGGATLICLIVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            34724..35398
                     /locus_tag="MJE33_001478"
     CDS             34724..35398
                     /locus_tag="MJE33_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9647419.1"
                     /translation="MARWNGFTWKWYGQLLQNESIMSALYYTIVIAILASVISTIVGT
                     ISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLFVFVKMEFGFTTMLLAHIM
                     FCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVILPQIKPGIVSGFLIAFTM
                     SIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALSTIMFAVVLGLLLLANKKES
                     IVRGIK"
     gene            35398..36450
                     /locus_tag="MJE33_001479"
     CDS             35398..36450
                     /locus_tag="MJE33_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HBY9647420.1"
                     /translation="MIKFKKAISLIAVGMMTVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPMDSWNILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITQNPVAYPSKEVLDKCETFEDIG
                     ENIKLYDKAWIELKSK"
     gene            36680..37528
                     /locus_tag="MJE33_001480"
     CDS             36680..37528
                     /locus_tag="MJE33_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBY9647421.1"
                     /translation="MNRINKKIDLLILALLVFIFLIVGIVILSIRTENKLNVFIMLAI
                     IFFIIMLTYLSNSVVGLITSSIIIFMYTSYILYNNITHNMDVEFISYMWIIATPVSSI
                     IMGNLNKSINELQNTNKKLSEQYKELVTIDSETGLRNLKIFYNDVNMEISKSIRHNTD
                     FSLMIVKLPYYGNLQTIFGENKTNKIVKYIGSNIIECTRNEDIIYSLQKDMIGILMPN
                     TSLEGSKVVKDRIKKRIKELNLDLNNRGKYVNIDVKIAFLQYKSSFGDSINFKNIVEE
                     ELQYDV"
     gene            37518..39182
                     /locus_tag="MJE33_001481"
     CDS             37518..39182
                     /locus_tag="MJE33_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBY9647422.1"
                     /translation="MMFKIFNKSLLKLVVVLSIVLTISNFSLSSYASEITSSTDKVLI
                     IYDSKKETAYNRDILNIMRTLLGRFSSDIELLKLSNYDGEINKNYYSHIFILGINENS
                     YNNDKNTKNLISSLNSYKGTICWLGYGIENLLEHKKYNLDYVGKTNNIVSVNYRGKSY
                     NLDEHYVFNIVESKDTSNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKTFDKGRIFVRIEDVHAFREPKNLVEIADYLSSKNIPFTIALIPAYVNPK
                     NHKVITLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLNGLRVCIENGIYPLAFEAPHYAMESEGYKELKKYFSTYIGQHQNND
                     KKFSTNTYPYIIRDTEEFNIFIPENLGYIDPEDKFTFQNIKENLDKLSIVRGFSGGFF
                     FHSYLNIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVDEIQIRNNGDEIIVNYDKDLDE
                     LTKSDTRFKSISNISKVLIFIVSISVLIFVIIFIYFKRIDKKKFLK"
     gene            39231..40487
                     /locus_tag="MJE33_001482"
     CDS             39231..40487
                     /locus_tag="MJE33_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBY9647423.1"
                     /translation="MRDLNVSEYLFVFSLFSIWSLLLINIILAMGGYIFYFKNFDKEI
                     KEIDEYPMISILVPAHNEAKVIGRTVESLLLLNYPKSKMELIVINDNSSDNSKEILEN
                     IKDRYNNYNFTIINTDSLTGGKGKSNALNIGYTISKGDFIAVYDADNTPDKNALRYLV
                     QTIVMNDELGAVIGKFRTRNKNKNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTN
                     FILRRSIIEEIGGWDSKAIAEDTEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQ
                     RTRWAKGNIYVLMKYIKNIFKQGRNKIVFDIAYFFSVYFLFLTSVIISDILFVLSISK
                     LVEISIPINFFLIWILSYLLFIIEVSISLTIEKGEATIENIFIVAIMYFTYSQLWLFV
                     AIKGMIEYLKDIIFKREVKWYKTERF"
     gene            40510..42591
                     /locus_tag="MJE33_001483"
     CDS             40510..42591
                     /locus_tag="MJE33_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HBY9647424.1"
                     /translation="MKKFIISIISLVLFFSNISLIYKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEEVLLHLNFSKSQILNGDVSSLTVLINNVPIKSIKLNAKTN
                     YKNTLEVLVPKDYIIQGYNEIKIKTYKTISDKICQDDSNTGNWMVIHKESYISIRYKQ
                     KKVENSINEYPYPYAEIENNHKLDTTIVVPDNMTRGETTAVFNLASAFGKITKNDDLK
                     LDVKLYSEMKNWSDDNIIYIGKPENTAEEILDILSIKEQTLLSSNCIIKQVDSPYNKN
                     KKMMVVIGSNEDDLIKASNLLIENRLSNQVLSSSVLVNKETNIKINREQKLNLGHLTL
                     KDLGYSDFLLEGAFNQQALFDVKIPTGKVLDDGSKIILNLRYSDNLDFEKSLVTVSIN
                     DVIVGSKKLDRSHSNNDKLELKIPKDIDNKNYYQVKLTFNLSIKNSNCVTRESNNPWA
                     YVSNNSYLALSTKENETLSFENYPYPFVRDDEFNDLTVIMPDYSGSQAMTWMFRLGVT
                     LGANINSHNGNINVIRGKEFSDKYKDTNIVVFGVPHNNSVIKMLNNNLNIKFDKNYSN
                     FISNDKISFIDDYGKNISTIQLIKSPYNNQKNIMVISSMNEKNLYLGMDYLLNKSKVN
                     DLKGDTLIIDEYGEVEDLAYNLKSKKEVKDSSWNMSINKTTKVFLMISFITIIIVMIL
                     SMLYIKKYKRR"
     gene            42628..43713
                     /locus_tag="MJE33_001484"
     CDS             42628..43713
                     /locus_tag="MJE33_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBY9647425.1"
                     /translation="MRKYMNEFKLKRSILISLIILIFIFIVYFVSIELKDRSLVNNQY
                     KGKIKSANLSVDYEINQVMKDIDKLGLNTVNVPIIINVESINSDIMSIDNNSKEKAIK
                     LIKKLNKKGISTILEAYPWIENGKLYETDWNPINKKRFFYTWQNAILNELIIDVANPL
                     DVNVLNIGSNFVHLEEYQQNWGEIIDFVQSKFDGLVTYRTNWWYTKKDDLSSKLFYEQ
                     KLNNNFFDKLDFISIAAYFELSNKPVNTVDELISALHSSTVNNRGQNIKQEIYNLYKA
                     HRKPIFFGELGFSNRESASSQPWNHTPSKAVNGEEQARCFEAYKKVFENEDWINGFSV
                     FCVGKIDDEKNFYPSKESIKVIKSWYE"
     gene            43767..44891
                     /gene="wecB"
                     /locus_tag="MJE33_001485"
     CDS             43767..44891
                     /gene="wecB"
                     /locus_tag="MJE33_001485"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HBY9647426.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRENIKSIVCVTAQHRE
                     MLDQVIETFNINVDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIILVHGDTTTT
                     LATSLTAFYNKTLVGHIEAGLRTYDKYSPFPEELNRQLTGIIADMHFAPTNLARKNLI
                     SEGKPNNNIFVTGNTAIDALKMTIKENYNHPIIDEIGNDRMILLTSHRRENLGKPMKN
                     IFRAIKRIVDDFEDVQIVYPIHLNPKIRTIADEIFGKFPEKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGIQEEAPSLGKPVLVLRDKTERTEGIEAKTLKLVGTNEDRIYNSVSDL
                     LINKDNYVQMSKASNPYGDGNASKYIVDIIIKKFNCKYLN"
     gene            44969..45943
                     /locus_tag="MJE33_001486"
     CDS             44969..45943
                     /locus_tag="MJE33_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBY9647427.1"
                     /translation="MARKLIKNLGKSKSVKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     DIGEKFIVEDKNGKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKKLEDSAIREYIDYGILPSITIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQALENAGYSTAEDKKGNRIYAELLIDVIRSYNLQLIDNKVETK"
BASE COUNT        18378 a         4669 c         7992 g        15047 t
ORIGIN      
        1 tatttaattc taatacagga agaaggtaaa tgtatattga aaaaatcata gaaatagaag
       61 taaacaattt aaataaatac aaaggtttgc aaaagaagta atataattat atacaatgag
      121 gatgtgagta tgatgaacag actaaagcca agaattttag gtattagtat cttgtttatt
      181 attataatta ttttaacatg tggagctgtt ttcaagttta aatttaaatt agataataaa
      241 ttaaaagaaa ccacacatac atatttgtct gagtttacag aacacaatat aattaatctc
      301 aagacaagac ttagtggtca atttgacatg cttgaatcta ttgctaactt cataggaaac
      361 ttgaataata taaatgatga aatggtgatt gatttaatgg attctgatgt aaagcgaagt
      421 tctttaatta gaatgactat ctctacatta gatggggaat gttattctaa tgatggaatt
      481 acccataatg ttcaagatag agattatttt aaaaaagcta aaaatggaga aaagaatata
      541 tcagagcctt taaaatctat tgttaataat aaagaaataa tagttttatc taccccagtt
      601 tataaaagtg ggaaaatagt tggtgtttta agtggaattt atgattcaaa aaagttaaat
      661 gagttattag gattatctgc atttgatgaa aaagcagatg tttttgttgc aaagaaaaat
      721 ggagatatag ttgctcaaaa aacaaataaa aatagaaaga cgctatcatg tatatttcag
      781 ctttttgaca aagataattg tgatgaagat gattgtctaa aaactataaa agaaaatatg
      841 aaaaatgata agtctggata ttttaattat aacttagata atgaaaaatt tatgttaaac
      901 tatcagccac ttggagttaa tgattggtat gttttttctg ttgtgccaaa taaaatagta
      961 tctattcagt cagagaatat atcttatgat gtatatattt ttgcattaga aatactacta
     1021 gttatattga tttttataat atatatagca tgtattataa ataataacac caaaattatt
     1081 ataaatgaaa aagaaaaatt taaagtactc actgataata tattaggagg ggttaaaacc
     1141 tgtttaaaag atgaatatat gactatgaca tatgtaagtc aggggttttt agagcttaca
     1201 ggttactctt gtgaagattt aaataaaata tttaaaaata gatttaaaga catgatttat
     1261 aaagatgatt tagacaagtt taattgtatt attctaaatc aattagaaaa acaagataaa
     1321 attgaagttg aatatagaat tgtaagaaaa gatggaagta taatttggat tttggagaga
     1381 agtaagtcta taaatgataa taaaggaagt gagtgtattc agggagtttt aactgatata
     1441 acagaactta agaaaataca agaagaatta gattcaaaaa gatttgaaat acaggctatc
     1501 aatgatagta ttgttggtgg aatagtgata actgagataa atgatgattt tgatagtata
     1561 tatttaaatg aaggttattt aaatatgatt ggatatacac gcaaacaact ggaagatgag
     1621 ctaaataata aattaatcaa acttattttt aaagatgata gaacagaatt aattaatagt
     1681 ataaacaaac aactcaaatt aagtgataca tttgcatctg aacaaagagt tgaaaaaagg
     1741 gatggcacta ttatatggat tttattaaaa ggaagaaaga tgatttatga taatggaaaa
     1801 tctgtacttt gttgtgttat tgtagatatt acttctgcta aattattaga gcaagaactt
     1861 aaaaagagtg aagaacgtca tgaaatcatt ctaaagcaga cgcaaaatat tataatagaa
     1921 tgggatatag aaaaagataa aatgaatata tcacctaatt ggtataataa gtttaaatat
     1981 gaccctataa ctgaaaacgc aacaaaaaat ataattaaat cagtagatat atatcatgat
     2041 gatatggata agtttatttc attaattaaa cttacttcac aaggtagtga gtatgaagag
     2101 tgtgaaatta gacttagaac tagagatact atttacgctt ggtttagtat aaaggcaaca
     2161 actttatttg ataataatgg taaaccttat aaagtagtag gaagtttgac tgatattgat
     2221 gaatcaaaac gagaagctga aaagttgaaa gaaagggcag aaaaagaccc tcttacagat
     2281 ctgtataata aaaaagcatc acaatcactt atagaggagt acataacatt ggataaaaaa
     2341 agaaaaggtg ctttaatgat tattgatatt gatgacttta aaggaataaa tgataattta
     2401 ggacatttat atggggatgc tgtattaagt gaaattgcaa atgacctaat aaatctattt
     2461 agagcaaccg atatagtagg cagaattggt ggagatgagt ttattgtatt tataaaagat
     2521 atttctgaaa caagagatat tataaataaa gcagaagaat tggtaaaaac atttgaaagg
     2581 tcttttttag gtaaaggacg aaattataaa atatcattaa gtgtaggaat tgctaagttt
     2641 ccacaagatg gtatggacta tatgacacta tttagaaatg cggatatagc tctttactca
     2701 gccaaaggga agggcaagaa ttgttataca ttgtatgata aaaaatttga taaagtccaa
     2761 tatataacta atacaagaaa tgcaggagat agtgataatg tagagatgtc ttttaaagaa
     2821 catatcacaa attatatatt tgatatacta tatgaatcaa gtgatataga agtggcagta
     2881 aatttaattc taagtattgt aggtaaacac tttaatgtga gtcgcatgta tatatttgaa
     2941 aattcagatg ataatcgagt atgtaaaaat acatttgaat ggtgtaacga aggtgtaacc
     3001 tcggaaatta ataatcttaa aaatatttgt tacaaggatt taggcaacta tcttttaaat
     3061 tttaatgcag atgggttatt ttattgtaat gatattgaaa atttaggtag tgatgtgaaa
     3121 aaagtactta agccacaagg catacgttca atattacaat gtaaaatttt agagggtaat
     3181 gaatttagag ggttcatagg atttgatgaa tgttcatata atcgtatgtg gactaatgat
     3241 gaagtgaaca ctttgacatt tatatctaaa acgctcagta tgtttttatt aaaaatgcgt
     3301 attcagagta agctagaaga gtctttaaca attcaaaaat ctgtgatgga taatatggat
     3361 atatgggcat atgttgttga caggaatacc tatgaattac tatttatcaa taaaaaaatg
     3421 cttgagtcag caccaaacac tcatgtaggt gatttatgtt ataaagcgat atggaataat
     3481 caagataaac cttgtaaaaa atgccctatg atatatttaa aagatggtga tgatacttat
     3541 aaatcattca tgcataataa aaagtttgag gtacaggcaa atataactac aactactttg
     3601 aaatggtttg atggtaaaga tgcatgttta atatttggaa gtgaaataac tagtattgaa
     3661 gctgaagaaa atacttaaaa tactgtgtta tatatatact taaaatcgca atttgaattt
     3721 attagactta aaatgtctat ataaatcatt taagttgtgg ttttaagtat tttttatgat
     3781 ataattaagc gttaagaaca aaattaaaag atacttttgt ataaaaaaat atttgttaaa
     3841 tatatattga gcaggtgata atatggaaaa taagttttta ccaataagca aacaagatat
     3901 gatagataga ggatgggaag aattagattt tgttctagtt acaggagatg cttatgtgga
     3961 ccatcatagt tttggaacag caataatatc tagagtttta gaaagtgctg ggtataaagt
     4021 tggaataata gctcaaccag actggagaac tacagatgat tttatgaagc taggaaaacc
     4081 tagacttgca tttctagtta atgcaggaaa tatggattca atggtcaatc attattctgt
     4141 aagtaaaaag catagagaaa aggatatgta ttctccaggt gggaaaatgg gttatagacc
     4201 agatagagct actatagtat attgcaataa aataagagaa gcatacagtg atgttgcaat
     4261 agtaataggt ggaattgaag cgagcttaag aagatttgca cactatgatt attggagtga
     4321 taaggttaga aagtctatgt taatagatag tggagcagat ttattagttt atggtatgag
     4381 tgaaaagcag attgtagaag tagccaatgc acttaatgat ggatatgacc caaaatacat
     4441 tagacatatt gatgggacat gctatatttc agatacattg gaagaaatat atgataaata
     4501 tatattaatt ccatcatata aagaaatttg tgaagataaa atgaagtatg tagaagcttt
     4561 taaaattcaa tatgatgagc aagacccatt tagaggtaat ataattgttc aacctcatgg
     4621 aagtaagtat ctggttcaaa ataagccaga aaaaccactt agtagagaag aactagatga
     4681 agtatatgga ttaccatatc agaagacata ccatcctgtg tatgaaaaat ttggaggaat
     4741 accagctata gaagaagtaa aatttagtat agtaagttca agaggctgtt ttggtagttg
     4801 ttcattttgt gcaataacat ttcatcaagg tagagcagtg caaagtagaa gtgaaaaatc
     4861 aattattgat gaagcaatag gaataacaaa tttagatgat tttaaagggt atatacatga
     4921 tgttggaggt ccaacagcta attttagaag accagcatgt aaaaaacaaa ttactaaagg
     4981 tgcttgtaaa aatagacaat gcttatctcc atcaccatgt aaaaatttag atgcagacca
     5041 cagtgaatac ttacatttac ttagagctgt aagaaaatta cctaaaataa aaaaggtgtt
     5101 tgtgcgttct ggaattagat acgattatgt aatggcagat aagaacaata aatttcttag
     5161 agagttgata gagcatcatg taagtggtca gttaaaggta gccccagaac atatatcaga
     5221 agaagtttta aagtatatgc aaaaaccagc aggaaaaact tatgataaat ttagacagaa
     5281 gttttttgct ataaatgaag aattaggtaa aaagcaatat ttgataccct atttaatgtc
     5341 tagtcaccct ggttctacct taaatagcgc tatagagtta gcagagtatt taagagatac
     5401 tcactaccaa ccagagcagg ttcaggattt ttatccaact ccagggactc tatctacaac
     5461 catgttttat acaggtattg acccgcttac aatgaagcca gtttatgttc ctaaatcaaa
     5521 acgtgacaag gcgatgcaaa gggctttatt acaatataga gcacctagaa attatgattt
     5581 ggtatactct gcgttagttg aagcaggaag agaagactta attggttttg gacatagatg
     5641 tcttataaag ccaaaaaatg aaaagcctta ttttaataga aataattcta aaaaaaatgt
     5701 atctaaagga acaaataaaa acaaaaaaac taatacaaat aacagaagta ataaaaatca
     5761 acaaaaaagt tcaactaaaa aaaagaaaaa acattaaagt ataaataaaa atacttaaaa
     5821 aagaaggatt attcgataaa ttaacagaaa ttagtataaa taaatataat tattttaatg
     5881 acgttacctt tgggaggaag agatgaaaaa gtatatttct ataatattat tagtggtgtt
     5941 gactatggtt gttttggtag gatgttcacc aggaaaagat aaccctaaaa ataaggagct
     6001 atcagttgtc aaacaagtaa aagtagctgt tccagatgga ctaccagcag tggcaatagc
     6061 taaacttgca aatgaaaatc cagagattaa agaaggatat gaaacagtgt atagcattga
     6121 aaagacacct gaagcgattt ccactagagt gatgaaaaaa gatgcagata tagctatagt
     6181 tccttcaaac atggctgcaa tagcgtataa taaaacttca tcatataata ttgttggaac
     6241 tgtaggaatg ggttcacttt atttggtgtc aacagaaaat ataaaagatt attctgattt
     6301 agttggtaaa gaagttggat gtactggaaa aggacttact ccagacataa ctataaaaag
     6361 tcttttgtta caaaaggata taaattattc agatataaaa tttaattatg taaattcagc
     6421 aagtgaactg gtaccattat tggctactgg taaagttaaa acaggtattg ttccagaacc
     6481 tgcacttagt gctttgatgt ctaaaaatcc agacataaaa attataaaaa gtttaaatga
     6541 ctcatggaag gaagtaagtg gttcaaaaga tggttatcct caatctactt taattgtaaa
     6601 gtctgaattt ttaagagata ataaagattt tgttgattct tttgtaggtc aattatcaaa
     6661 tagtatagat tgggcaaaca aaaatcctga agaattaggt gcatattctg aaaaaataaa
     6721 aatatctact gaatcaaaga taataggaaa atctcttgaa agagctaatt taaaatatat
     6781 tccagttaag gacatgatta aagattataa aaattactat gagaaattag ctaattttga
     6841 tgataagaca ttaggaggca aagtaccaga tgaagcaata tactttgttg agaaataaaa
     6901 aaataaagaa caatttatag ataaaatgga ggtaacactt tcttgtgtta tcctcttatt
     6961 tttatggcaa attattgctt taaaaattga taatgatatt tttttaccaa cagtaggtca
     7021 agtatttaat agtataaaag agattatgct aaattctagt ttctatatag atatacttta
     7081 ctctatgggg agatgttttt ttagtttttt actggctatg atttttgcta ttatactttc
     7141 aatagtgtct tatttaaata gattttttag aaacttatta aagccaatca atgctttgac
     7201 acgttcaata ccaactatga tattggtagt acttgcactt atttggtttg aaaaggatag
     7261 tacccctttt atagttggat ttactatagt ttttcctata ttgtatgata acgttttggg
     7321 cgctatattg aatatagaca agaacttatt agaaatggct aatgtgtata aaataaggtt
     7381 tatagataag gtgttaaaaa tttatttacc agccataaaa tttcaaatta tatctatact
     7441 agtatcaaca ttctctttaa gtttgaaagt tgctatagca ggagaggtat atagtcaacc
     7501 tacatatggt attggaacta tgatacagac tgaaaaaatt aactttaaca catctggaat
     7561 atttgcatgg ataattattg ttcttttaat atctgctatt ttacaaatag ctcaaaagtt
     7621 tttagcaagg agagcttttt tatggaaaag ataaagttga gtattgaaaa tataaataaa
     7681 agatatgata gtcgtattat atttagagat tttaatattg atttttatgt aaatgaggta
     7741 aattgtatcc ttggaaaatc tggttgtgga aaaactacac tcttgaacat aataagtggg
     7801 attattaaaa atgacaccaa caatctaaat atcaaagaaa atttaaatag agtaggaaat
     7861 aagcttgaag ctagttacat atttcaagat gatagactta tagactggtt aacagtagaa
     7921 gaaaatataa agatagttgt aaataaatac tataataaaa ctcaattaaa taaaatttgt
     7981 gatgaatact tggaattggt tggaattagt gattataaaa aattctatcc tcagatgctt
     8041 agtggaggta ttagacaaag agtaaatatt gctagagctt ttatatatcc atcaaaaaat
     8101 attataatgg atgaaccttt taaatctatt gatgcaaaga atacacagct tataatggat
     8161 aatttcagaa atatattaag aaaagaaaaa agaactgtgt tatttgtaac tcataatatt
     8221 gaggaagcat tgtttttagc agataggata tttattttag gagactctcc aattagaata
     8281 aaaaaaatat taaaaaattc taaggaattg gaaaaaaatg aagttttgaa gttgatatag
     8341 agttataata atactgtcgt tttaaaatat atttggttaa aatctataag actatattaa
     8401 aatagtacag aatattcaaa actgtgtata atgttcgtat tttataaaaa ggaagtgaat
     8461 gtatggttaa gactaatgct atgagaatat tagattcaaa taagattgat tataaagtaa
     8521 tgtcttatga agtgaaaagt gaacatgtgg atggtgttga ggtggcacat gatataggaa
     8581 gagatgtaaa tgaagtgtat aagacattag taactcaagg agttagtaag aatatatatg
     8641 tatatgtaat accagtacat gaaaacttag acttaaaaaa ggctgcaaaa gtagctaaag
     8701 agaaaagtgt agagatgatt catgtaaaag atataaataa gcttacaggt tatattagag
     8761 gcggatgttc tcctataggt atgaaaaagc tttataaaac ttttgtaaat gaaagtgcaa
     8821 agaatttaga tacaattatt gtgagtgcag gaaaaatagg atatcaaata gaactatctc
     8881 catttgattt acaaagactt ataaaagtag aatttgtaga tgtaataaaa aaataagaat
     8941 tacatataaa atatagagct tatattctta ataataggtg ttgtagtatt aataaaatga
     9001 ataataattt taaaataaat attcagttac ttatgtgtaa ttggtataaa aatattgaga
     9061 aattttaaaa acaatggtaa agtgtaagta taatcacgaa tattttttat aaagtaatgg
     9121 agggatgttc gcttgagaaa aataaaatct gaacaaatcg tcgagcaggt aaaaaaactt
     9181 tgtatagaag cgagcctata tttaggagaa gatgttttaa gctgtataaa agaaaaagcg
     9241 aaaagtgaaa agagtgaagt tggaaaaaat atattgaata tattagttga aaatgctgaa
     9301 atagctaaag aaaaaaatat accaatatgc caagatactg gtatggctgt attttttgtt
     9361 gaaataggac aagaagtttt aattgaagga gacacactta ctgatgctat aaatgaggga
     9421 gtaagacaag ggtatgaaga aggctactta agaaaatctg ttgtaagtcc aattaacaga
     9481 gtaaatacaa aagataatac acctgctgtt attcattatg atatggtaaa aggtgataaa
     9541 attaaaatag aatttgcagc taagggattt ggaagcgaaa atatgagtaa gatgaaaatg
     9601 ttaaagccat cagatggatt ggaaggaatt aaaaaattca taatagatac agtttcagaa
     9661 gctggaccta acccatgccc tcctatggta attggagttg gtataggtgg gactgtggac
     9721 aaatgtgctc aaatagctaa aaaggcactt tttagagaac taggtgaatt taataaggac
     9781 gaaaatatag caaaacttga aagtgaacta ttaacagcta ttaataaact tggtatagga
     9841 cctcaaggct taggtggtac tacaacagct ttgggattaa acatagagac ttttcctaca
     9901 cacatagcag gtttacctgt tgttgtaaat ataaactgcc atgcatcaag acataagaag
     9961 gtagtaattt aggaggaaaa taaaatgata aaaataacta cacctgtaaa tgaaattgat
    10021 atagcaaaat taaattgtgg agatacaatt agtttgagtg gtatacttta tacagcaaga
    10081 gatgctgctc ataaaagatt gatagattgt ataaataaag gtgaagaatt accatttgac
    10141 gtatatggtc aaggaatata ttatgtagga cctactccta caaagccagg agaagttata
    10201 ggtgctgcag gtccaacgac gagttataga atggatgatt tgactatacc tcttttagaa
    10261 agaggtctta gattgatgat aggcaaggga aaaagaagtg atgaagttat agaaggtatg
    10321 caaaaatacg gagcagttta tcttgctgca ataggaggag ctggagcata tatttcaaat
    10381 tcaataaaat catgtgaaat tattgcatat gaagatttag gagctgaagc tataagaaag
    10441 attgtagtag aagaccttaa gcttacagta gcaatagata gctatggaaa taatatatat
    10501 gagcaaggtc gagcaattta tgaatgtaaa taatgtttag aaataaatat gaatatcaaa
    10561 attaaaaata gttgtgtata ttttaaggag gaaaattgat gtctaaggat tatgcaaaat
    10621 tggcattaga aatgcatgaa atcaacaaag ggaaagtaag tgtagaaagc aaggtagaag
    10681 ttaaaactaa ggatgattta tcaacagcat atacaccagg ggttgctgaa ccttgtttaa
    10741 aaatacatga aaatcaagat gatgtttata gatacacttc taaagggaat ctagtagcag
    10801 tagttagtga tggaagtgct gtacttggat taggtaacat tggtgctgaa gcatctatac
    10861 ctgttatgga aggtaaagct atattattta aacaatttgc agatgtagac gcttttccaa
    10921 tatgtttaaa gactaatgat gtagatgaga tagttaaatc tgtagagttg atggagcctg
    10981 tatttggagg tataaatttg gaagacatat cttctccaag atgttttgag atagaagaga
    11041 gattaaaaaa atctttaagt atacctgttt ttcatgatga ccaacatggg acagcaataa
    11101 tagttgcggc agccataata aactcaataa agttaattga aaataaaaaa atagaagatt
    11161 tagaaatagt tataaacgga gcaggagctg ctggtatagc catagcaaag atactactta
    11221 atatgaatgt taaaaatata atcttatgtg atagaagtgg agcattggaa gctagtatag
    11281 aaaatctaaa ctatgttcaa aaagaaatgt taaaagtaac aaatataaga aatgaaaaag
    11341 gacctttaaa agatattata aaaggaaaag atgtatttat tggtgtttca ggtccaggag
    11401 ctgttacaaa ggaaatggtt gaaagtatgt ctgagaagcc aataatattg gcaatggcaa
    11461 atcctactcc agagataatg ccagaagaag caaaattagg tggagcattt ataatgggaa
    11521 caggtcgttc agacttccct aatcaagtca ataatgtact ggcttttcct ggaatattta
    11581 gaggtgcact tgatgttaag gctaaagaga taaatgaaga aatgaagata gctgctgcat
    11641 atgcaattgc taatacaata agtgatgaag aaatatgccc agagtatata ttaccagatg
    11701 tatttaataa gaatgtagta aaaaatgttg ctaaggcagt taaagaagca gcgataaaaa
    11761 ccaaagtaaa tagaatataa atttttatat gttcatatat ccttatatta tcgggtatat
    11821 gggcattttt attataggtt tatactcata tatccttact tttatataca tatctttatg
    11881 attaaaggtg ttagaaaatg tagtaaattt tatactatca gactctatct ttattacttt
    11941 agtagataca tggttgtaaa ggtttataga aaaaaatata ggaaggcaga tttttattgt
    12001 tgcgtatata tgagaatgat ataatatgaa tattataaat taataaattt aaatttatgg
    12061 aggtacaaga gatgatggaa tataaagtta atgaccaaga acttaatgct tcaatgttta
    12121 tcccattcgt caacaggata tggccaggag attatgatat ggataaaaca cagactgcat
    12181 tatcaaaaac tttgaatata actgcttatg atgataaagt gcttgtagga tgtctgcgag
    12241 ttctttcaga tggttattat tttggaacaa ttacagaatt gcttgttctt ccagagtatc
    12301 agaaacaagg cattggtagt aagcttttcc agcttgctaa agagaacacc ccaactatgc
    12361 tatattttgg tgcacagcca ggagtagaag agttctatga aaaaaatggg tgccaaagaa
    12421 gtctgcaatc ctacataata aaaaagaaag atagttatta aagttccagc tttcaataat
    12481 cttaaaagta caatatttta gtggaagcat ttcatttttt attactaata tgacataaac
    12541 atgtcatatt agcattgata tacttttaat atggttaaga gccaaagaaa aatataagga
    12601 gtgatttaaa atggaagaaa ctaaattttg ccaatgctgt gcaatgccaa tgggacaaac
    12661 tgatgaatta tatgggacaa ataaagatgg aagtaaaagt actgattatt gtagttattg
    12721 ttttaaagat ggtgagttta cagctgatat aagtatggaa gagatgatag aagtttgtat
    12781 ccctcatatg cttcaaagta ataaggatat gactgaagat gaagcaagaa caatgatgaa
    12841 caatttcttt ccaacattaa aacgctggaa ataattttat gcaaaattag ttatatgcaa
    12901 aacatgaaat atggtgttga gaataagggg tggtgtgctt tatagcatac cacctgtttt
    12961 gttacttgga gttcagttat tttcctaaat attttaaata tactatttta gataagttcc
    13021 ctcttcatct tgataaatta atcctttttt cataagcact ccaagagctc gtttaaaatt
    13081 tttcttactt gaattaaata atatagaaat atctttagga tcagatttat cattaaatct
    13141 catatatcca tcagaacctt ctaaataact taaaatctta ttttctaaag tatcaagttc
    13201 atcctttcta tttcctctag gagaaagacc aagttttcca tcttcatata tttttatgac
    13261 atgtaaattt tcaagaacat caccagtttt tagttcagta aagtactcat tatgaagtat
    13321 tacaccagca tatttattgt ctacacatat cattgcagaa ttattagttt ggaatccata
    13381 tacaactcca gttacaatat ctcctacatt gtaagtatgg tctgttaata aatatgaatc
    13441 tatatctgta gtagcagcaa ttcttttgct tttgtctaaa tatatataaa ataaatattt
    13501 ttcgtctcta aatagtggat aagtcttagc tttaaaagga actaatatgt ctttaggaag
    13561 tcccatatct ataaaagtac caatgtctgt attatcaact acttttaaat gtgctacatc
    13621 cccaacctta gctaaaggcg gtattaaagt tgcagcagtt ctgtcatcag aatctttaaa
    13681 tatgaaagca tttacttcat cacctatgtt tatttcattt tttccgatta atctattatg
    13741 aagaagtata tcgtcttttg tgttattagt ttgtccatcc aaaaaatatc caaattcagt
    13801 ttttcttttt actgtaagtt tattaaaatc acctatgttt atcaaattaa aatcatcctc
    13861 tcattttata tttataattt agccataata tacttatgta gtagtttgtc acagtatcta
    13921 tattagtatt agtgaaattt acaataaata atatagttat ttatgtattt ttcaattctc
    13981 tagcttctta tatagtataa actccaattt cttttttgtc tttaggtttt attaaaattt
    14041 ttacaaaata agttaaaaag tattgaaata aaatttcttt taaagtataa ttggtatata
    14101 ccaaaattgg tatataccat atcgaaaagt ggtgactata tgaaaatagt aaataaatct
    14161 agtaatatac cattgcatac tcaattaagt tcaataataa gagagatgat agaaactggt
    14221 gagcttaaag agggtgatgc aataatgcca gaaagagaac tttgcaacat acaaaatgta
    14281 agtcgtatga cagtaaataa aaccattgtt ggattagtta cagaaggact tctttatagg
    14341 gtacaaggta aagggacatt cgttgcaaag caaaaaaaga agtatcagtt ttcaaatgta
    14401 aaaggtttta ctgatgttat gaaagaaaag ggagtaaaca taaaaacgga tatattatcc
    14461 tttgagatgg aactaccaga tgatttagtt aaaagaaagc ttggaataag tgataatact
    14521 acaaatatat ataagattgt cagacttaga tatactgatg gagagccatt tggattagaa
    14581 atagtatatc tatcagaaga aatgtgtaag ggtctaacaa aagggatatt ggataattct
    14641 tcattatata gggttttaaa tgaaaaatat ggctataaga tacaaaaagc tgaacaggta
    14701 atggagccag taatactttc agatgaagaa agtaagcttc ttgaaacaga tgaaggtgct
    14761 ttagctctta aacttcatag aaattcctat aatagagaag gtagccctat agaatatacc
    14821 atatcaatat ttagaacaga taaatatcaa tatgaaatag tattgagtga gtaggtgaat
    14881 aatatgaaat gtttaattaa tggaaagata atattaaaaa atcaaatctt agaaaataaa
    14941 gtattagtat ttgatgaaaa aataattgat atagctgata gtgtaccaaa agattgtgaa
    15001 gttatagatg ctgatggaaa atatatatct ccagggctta ttgatattca tatacatgga
    15061 aacatgggaa aagatacaat ggattcgact gatgaatcta ttgaaacaat ttcaaagtca
    15121 attatgagac atggagtaac atcttttctt ccaactacaa tgactatgga taaagaacat
    15181 gtttatgatg cacttgaagt tattaaaaaa gcccaaaata gaaagcttga aggggcacaa
    15241 gtattaggag cacatttaga aggacctttt ataaatgaga attataaggg agcacaaaat
    15301 gaaaaattta taataaattc aaaatacgaa tttataaaag aatataaaga tgttataaaa
    15361 gttataactt atgctccaga aaaagatatt gattttgatt ttactagaga aataaaaaga
    15421 tgtacagata tagttttatc tattggtcat agtaatgcaa attatgacca agctaaagaa
    15481 gctataaatt taggagtaac taatgtaact cacatgttca atgcaatgac tggtttaaac
    15541 catagagacc caggcgttgt tggtgcagca cttacaacta atgtgtacag tgagttaata
    15601 gcagatacaa tccatataaa taaagattta tttcaattta tactaaataa taaaggaaaa
    15661 gaaagactga ttttaataac agattctata gaagctggtg gtcttgaaga tggcaattat
    15721 tctcttggag gtcagaaagt catagttaag ggtaatgaag ctagacttga aaatggagca
    15781 ttagctggaa gtgtgttgtc tttaaataaa atggtattta acttcttaga taatacaaac
    15841 cttaaagtga atgaagcaat taatttagct tctttaaatc cagcaacatc tttgggaata
    15901 aatgacaaaa aaggaagctt agaaataggt aaggatgcag atatagctgt atttgatgag
    15961 aatttagatt gtaaaatgac actttgcttg ggggacgtag tatataaaaa tatatagtat
    16021 acaaaacaaa ccttaaagac agtaaaaaca atataaaaaa taataaatta aacttacatt
    16081 agtaaaagta gtagttaaaa tttaggagga ataagatgag agtattagtg tgtaaagatt
    16141 atgatggaat gagtaaaaaa gccgctgaaa tgatagcagc tcaaatagtt ttaaaaccaa
    16201 attcaatact gggactagct acaggaagta caccagtagg tatgtataga gatttagtaa
    16261 aaaaatacaa tgataatata gtagattttt cagatgtgat gagctttaat ttagatgaat
    16321 attataagtt accaatatct aatgaccaaa gctatgatta ttttatgaaa gagaatctat
    16381 ttaatcatgt aaacataaag ccagaaaata ctcatctacc aaatggaatg gctgatgata
    16441 ttgaaaaaga gtgtatgaac tatgaagctt ctatagatgc agctggtggt atagatgtac
    16501 aagtacttgg aataggtcgt aatgctcata taggttttaa tgaacctgat actaaatttg
    16561 caaagagaac tcatgtagtt gaacttacag agtctacaat agaagctaat gcaagattct
    16621 ttaaatcaag agaagatgtt cctaaaaaag ctgtaagtat gggaataggt tcaatattga
    16681 agtctaagaa aatattgctt ttagcttcag gagaagaaaa agctgatgca gtatataata
    16741 ctgtttatgg agatataact ccagaagttc ctggttctat acttcaactt cataaagaca
    16801 ctatagttat agtagatgaa gcagcagcaa gtaaattgaa tccaaaagat tataaattag
    16861 tataagaata gaatgtagtt taagtaaaaa atagctatca ttgagtaatc aaatttattt
    16921 aatgatagct atttttattt aaatttttta cttcttttta tttaaattag ttattattgt
    16981 ttaaattact tgcttattac tttttacgaa aactcgttat caagaagtat ccaaataaga
    17041 tgttaaaaat tattatgttt agtgggattc tatcaagcat aataaaatta cctataattg
    17101 ggatattacc aactggaaac ataaagaaga atacaggcat cagtgatagt aaaaaagatg
    17161 gaaaacaaag aagaagtaat cttggaatat caatatacca actaccatcc tttttgtatt
    17221 ctctaatcaa taattcagtt cctagtaata aaccaatacc accagaggaa attaaattaa
    17281 taaaaatgta tcttttaaag tcttgcatat aaccagtgta aaattgagta tagaaatcat
    17341 aatttaattt aacggctaaa aaatacataa gcaatagaat gattaaaaat aaaagatatc
    17401 taagagcttt tttcatttta aattctccct tcagaaatac tttatttagt tttattttgt
    17461 tggaatctga ttttatcatt tagtttaaga aatccataat attagtgaag aatgaaataa
    17521 gtattatata tgaataaata tttttagaat taaggtgaat tgtagtgctt aatataacta
    17581 taatattaat tgtttgaata ttcaataaaa agtttgaaaa atatcaattc tgatataaac
    17641 tgaataaata cagttgaata ttaaattatt attttatcaa aaatatgcaa agtactacac
    17701 cttcaaatat cacctaaagt aattaaaagg caattaaggt gaagtagttc aagtatttct
    17761 aaaataagtt taataaattc tataataatt tattttttgc cttaaaattg ccttaaaatc
    17821 caaacaattt taaaactaat gtactataaa aagctggtat aatttaaata tagtaaaaaa
    17881 taagatttat cttatctatt gggatttaat tatttaatca atatatttat accatttaat
    17941 ttaaataaaa agtctattaa ggagtgagct tatgagtaag ttaatttata tagcagatga
    18001 tgaagataat ataagaaatt tggtaaagac attcttaaaa aatgaaggtc atgatgttat
    18061 ggattttaaa acgggtgatg aacttttaga acaatttaat ataaaggagt gtgacttagt
    18121 aatactagat ataatgatgc ctggctcaag tggttttgaa gtatgtacaa aacttcgtga
    18181 gaaaagtaca gtcccaataa tcatgcttac tgcaagagat acagatatag attatattac
    18241 agggataacc ttaggtagtg atgattattt tacaaaacca tttagcccaa tgtctttggt
    18301 tatgagagta aaatctatat ttagaaggat agagtttgaa aagaaacaaa attatgataa
    18361 atatagtaac tcgatagata tggaattaaa atttggtgat gtaataataa ataaaaagaa
    18421 taaaatcgtt acaagtaaaa atgtaaatat agaccttaca ccaaatgaat ataacctatt
    18481 gacttatttg tttgaaaata tagatagagc agtttccaga gatgaattat taaacaaaat
    18541 atggggatac gatatagaag ttgagacaag ggctgctgat gatacagtga agagattacg
    18601 taaaaaaatt ttaaatacaa atattttaat agaaactgta tgggggtttg gatttaggtt
    18661 gaaggagaaa agttaagatg aagaatggaa atgacaagaa tagaattgga aagattaaat
    18721 ccaaatttaa attaaagttc tcaaagaaaa atataagcat aaaaactagg attttgttga
    18781 gtatactttg tttgataatt atagtattta tgataatatt agtatctttt aatgtatttc
    18841 ttgatacata tataaaaaca actgcgaatg aagaattgac aaagtctaca gaaatagtag
    18901 aacatatgga tagtaatttt agacctatta agccaccaca agatgatgga aagcttcctc
    18961 ccaaagtatt atctaatttt atgagaaatg tacaagataa agttaggatg gcagaaacac
    19021 aatcagatgc tgatgctatg gtagttgatt ctaagtataa cctgatattt ccaacaagag
    19081 aggatgattt cttaaaaaat gttgatgaga tggagctgat tagggagtgt attcaaaatg
    19141 aaaaattaga tttaaattca gatgaaaata cacggatatc taccagaaat aaggattatt
    19201 atatttcaac tgtaaaaatt actagtatat atggtgaaca agacaagtat cttatacttt
    19261 ttatagacat atcaaaaact ctaaatctag cacaaaagat aaatattgta ttaatatctg
    19321 taatgtgttt tgctggaata ttagcaatat ttacagctgt gattttatct gagaaaattg
    19381 ctaaaccgat aaaagagttg tgtgagtttg caaaaaaaat gggtcaagga gatttcaaaa
    19441 gaacctactt tgacttttca gataaagaat tagtagagct ctcaactgtt atgaataaga
    19501 gtgctgagta tctggataag tatgataatg aacaaaaaat attttttcaa aatgcatctc
    19561 atgaattaag gacaccactt atgtctatta aaggttatgc agaagctata aaatataatg
    19621 ttatagatag taaacatgca agtgatataa ttcttgaaga aagtgatagg ttaagtgata
    19681 tggttgaaga tttattatac atttctaaaa tagataatat aactaaagat tttgaacttg
    19741 tagaatgtga tttaagagaa gtactttcaa attgtggagc taggcaaaat gtaagagcta
    19801 taaataaggg gataaaattt atttatgaat ttgatgaaga aatggtttta tttgaatgtg
    19861 atgaaaaaaa catttccaaa gcatttatga atcttataga aaatgctttg agatacgcta
    19921 agagtgaaat aaaaatagtg tgtaagtata atcaaaaaaa tatagttgtg ataatagaag
    19981 atgatggaat tggaatcaag aaagaagatt tacctcatgt ttttgaaaga ttctataagg
    20041 gagtaggagg aaatcatgga attggattat caatagttaa atctattgta aataagcatg
    20101 gtgggcgtat atacgtagaa aatggcaaaa aaggagctaa atttactata ttatttaaat
    20161 tgtaaaaaaa ttagagaaaa tatagagatg tttgaagttg ttttttatat atatctgttg
    20221 tatactgttg aaaaaagaac aatattaagc tgacatatat tacgatagtt gacatttaaa
    20281 gtaaatgctt aaatatagat acgaaaggaa aagtaaaatc tgaagtgtat ttaggtttaa
    20341 gtaagcttct tattttataa aaaatatgta tgaaagaaca gctgattttt aatattacaa
    20401 atttagtaac ggttgtattt gaagcttttg tcatccatat gtttttatca gactttttag
    20461 gtcagaaaga agggtatata aaagttgtaa gatatgctaa actgggtttt ataatatgtt
    20521 taggattctg taatttaata actttaaacc ctaaaatcac aatgccttta attttcatcc
    20581 ttattttttc aacatcattt ttatataaag gaaatttaaa gacacgattg tttactactg
    20641 ttttattaag tatattcttt atactgtcag aaattgtagt tacaagtatt tttgttttat
    20701 ttgtaaaaga aggctttgaa ataatgcttg aaaataatag tataagagta ttggcgacaa
    20761 ttttatctaa aattgtcttt ttgttgactt gtaaaattat ttgtctattt aaaaaggatg
    20821 tacatttaga tatgcccata aagtattggc tccctttatt tttaatacct atatttagtt
    20881 tatttctgtc tgtttctatc tttgatgtaa gtaaattttt tagtcttgaa agtttaaaat
    20941 ttctaagctt aatatctagt gttggtattt tatatattaa ttttattgtc ttttatttat
    21001 ttaaatttat tatagataaa actaaactat ctatgaaata tgaactactt gaaaaagaaa
    21061 taattcataa ggaagaatta agacttagta atgagtgtta taaaattatt gttgaacaga
    21121 ctgattctgt cgttttcgaa tggaatatca aagaaaataa atcatttgta tcgcaggctt
    21181 ggactgaaaa atttggatat aacaatgcat gtaagaatat ttttaaagaa attaaagata
    21241 aagacttagt tcattctgaa gataaagcta tatttgaagg ttttcttgaa agtattaaaa
    21301 aaaagaatat gcacaatcaa gcagtatatc gtttaaaaaa atctaatggt gaatatattt
    21361 ggtgtagaac atcaattaca agcatttata atgatgaaaa tgaactttta agagttgtcg
    21421 gagtaattgt agatgtagat agtgatataa aaaaatatga agagttaaga actagagcag
    21481 aaagtgactc attgaccaat atatacaata aaggtacatt tgaaaaatta gtggaagaaa
    21541 ctatagttat gaatacaggt gataaaaaag atgcattatt cattattgat ttggatgatt
    21601 ttaaagaaat aaatgataat tttggtcatc catttggtga ttttgtacta aaaacatttg
    21661 ctgataagat acaaactagt tttggtagta aagacttagt cggaagaatt ggtggagatg
    21721 aatttgttgt atatatgcaa gattatgtta cagaagtaaa tttgcataaa aaagctaaag
    21781 aattaaatag agtactttcg gataattaca ctgatttgag ttttagtttt gatgcttctg
    21841 tcagtatagg tattgcaagg tatcctcaag atggaacttc attttttgaa ttgtttaaaa
    21901 atgcagatag agcactttat agcataaaag catcaggtaa aaattcatat tgtttatttg
    21961 aagaagaact atatgtacag tgaatttgaa tttagatttt atgtgagagt aagatttata
    22021 tatacttgtg gctgtctgat ttataaaata tgttactttg tatattttta tatgtttcag
    22081 taaggttaat taacatgagc aaaggtgtaa gtaaatttat attagtttaa gtttacttac
    22141 acctttataa aaatacaaat aatagattca tttacattct taaatgatta aagctcttta
    22201 ccttctaaac atgcataaca tatcgttata attccagtaa ataagactaa aatgcccatt
    22261 actaagatta caatttcatc atctattact ccaaatgttt ttaaagtaac ttatttcgct
    22321 ttgtatcata ataatttgtc tctttttaca ttttaacttc tgctgagatt tgagttagat
    22381 attcattaga atctcgcaca cttatatcat atagaagata ttcttcataa acaaaatccc
    22441 caatacgtaa attattctga ttaaaaaaat ttaataactt ttcataagcc ttgtaaatag
    22501 tttcataact ccctttatga taagcagtta cataaagtcc ctttggtttg attgatacag
    22561 gatatttctt gtactcttta ttaacttttg taaataaata tgaatatgat tcaaagtctt
    22621 tattaattat atcttcacct ttaatgattg agccaatcgt accataatca ctcaattcat
    22681 agttgtccat aaagttaata cattcatata aaaaattttc ttcctcatct cctaaaaact
    22741 gttctttata tgtaacaggt gttttgacca agtactcttc ttcatgttct tttaaagtaa
    22801 tagtattcgc atctatatta catgcacttt tagtaaatga aatagtagat tccattagag
    22861 cttgaatatt atttagtttt tcaatttcat ttttaatatc aatggttttc ttttctaaaa
    22921 gctcaattag taaattaggt gttcttttgt ctatataagc tttaatttct tttaaaggca
    22981 tttttacttc cctaagtgaa gaaattatac caaaaatgct aagttgatgg tatgagtagt
    23041 atctatatcc attttctttc tttaattctg gtgagaaaag acctatatca tcataatgga
    23101 aaagtgtttt tttattgatt ccacaaatct ttgcaaattc tccagttgta aaataatttt
    23161 ttgaaacttg actcataatt ataattcctc cttgactatc ctgttacagt atagtttatc
    23221 atagtatttt gtaaaggagt gtgattatat gaaaaaatta attcattttt taaaaccata
    23281 tagagtttta attgttgtag ttttgatatt tacgttttta caaacacttg gaactttgta
    23341 tataccaaca cttacagcaa atatagtaaa taatggtgta gtaaagggag atatagatta
    23401 tattgtaaaa acaggtttaa tgatgatgat agtagctggt ataacagctt tatcagctgt
    23461 gttagtttgt aaagtatcag caaatctttc atctggattt tgtagagata ttagagaagc
    23521 agtatttata aaatcacaag atttatcaat aaatgatttt aataatatag gtacagcatc
    23581 aatgattact aggagtacta gtgatattac tttaataggt cagtctgtgt ttatgtttat
    23641 tcaattagta ttaccagcac caattataac tgtttctggt ctattcttag catattctat
    23701 agataaagca atgacaatta ttattgttgt agtaatgttt ttattcatgc tttcagcatt
    23761 tttagttggt aaaaagctta taaagttgtt taaaatgatg caaataaaga tggataatat
    23821 gaatagggtg ttacgtgagg tagtaactgg agtaagagtt ataagagctt ttaataggag
    23881 tcgttttgaa aaaaagagat ttgatagaac tgcaatagat tattctgaaa cagcaatttc
    23941 aataaataaa atatttgcag tacttatgcc tattgtaatg ttaataatga atttaggtat
    24001 agtaagtatt atttggtttg gtgggatgag agtcagtaat ggaaatatgg aaataggtca
    24061 tataatggca cttgttgaat attgtatctt aattttattc tatttaatca tgggagtaat
    24121 ggtatttatg tacataccta gagcaggtgc atgtgcagat agagtaaatc agatattaga
    24181 tatagaacct gaaattgtag atggaaatgg tcataaagat actgtctcag agagaggaca
    24241 tttagtattt aaaaatgtaa ccttttcata tgcacaatca gaagaacctg tgttaaataa
    24301 tataacattt gaagcaaaat ctggagaagt aactgcaatt ataggaagca ctggctcagg
    24361 caaatctact attgcaaata taataccacg tttttttgaa attcaatctg gagaaatatc
    24421 tataaatggt caggatatta agaagattcc tcaaaaagaa cttagagata agattggatt
    24481 tgtaccacaa aaagcttttt tatttagtgg aacaatagaa gaaaatataa ggtatggaaa
    24541 agaagatgca agtattgaag aagtaaagca tgctgcaagt atagctcaag ctgatgaatt
    24601 tatttatgat atggaagaca agtatgactc atttgtagca caaggtggaa ataatttgtc
    24661 aggtggtcaa aaacagagaa tatccattgc aagagcgtta gttagaaagc ctgaagtata
    24721 tgtctttgat gatagttttt ctgcattaga ttttaaaaca gataaaagac ttagaaaagc
    24781 gttgaaaaat gaaatcaaag attcatcagc tataattata gcacaacgta taagtacaat
    24841 catggatgct aatcagatta tagttttaaa tgatggaaaa attgtaggta ttggaaaaca
    24901 taaagattta cttgaaaatt gtgaagttta taagcaaatt gcggattcac aacttagtaa
    24961 agaagaactt gcataaaatg aagaattaat ctgatatttg gaggtgtatg atatgagtga
    25021 aaaaagaata gatgaacaag atatagaagt acttagtttt gaagatggca tgtcagcaga
    25081 aaaagctaag gatgcaaaaa aaactactaa gagattatta aaatatatgg caaaacaaaa
    25141 gtttaaaata ttaattattt ttatttcagt attgataagt tcagcgctta cagttttagc
    25201 acctatggtt atgggaaaag ctattgacca actatttaat ggaataaaaa ctgcagtcca
    25261 aacaggtaca aaattcagtg tgaattttag tactatggga gggatagtat caatacttct
    25321 agggttgtac ttaattagtg cagtatttat atatattcaa cagtatatta tgtcaggagt
    25381 tgcacaaaat ctagttctta gcatgagaaa agatttaagt gataaactca ataaacttcc
    25441 tcttaagtac tatgattctc ataaaaaggg ggaaacactt agtatagtaa caaatgattt
    25501 agaaaaagta gcagatagtt tacaagaagg attaatgcaa cttattacag cagtagttac
    25561 agtagttggt tctattgtaa tgatgataag tataagtgta ccacttacaa tagtatcagc
    25621 tataacatta cttgtttcat tgggtattac tgttgtaatt gcaagaaaat cacaagaaag
    25681 attctctgaa aatcaaaagg cactaggaga gttaaatagc aatatagaag agatatttac
    25741 tggtcaaata gtggttaaag catttagtaa agaaaaggat actataagaa actttaaaat
    25801 attaaatcaa aatctttata atgcaagtag aaaagcacag ttttcgtcat atgcaatatc
    25861 tccaattatt agatttataa acctaattgg ctatgttatt atagcagttg taggtgggat
    25921 atttgcatca actggagcaa tgactcttgg tagtatacaa gcgtttattc aatatgtaaa
    25981 tcaagcatca gagcctacta cagaaatatc ttatattgta aatatgcttc aagcagcaat
    26041 agcatctgca gagagagttt ttacagttat ggacgaggtg gaagagataa aggatgaaga
    26101 atcttcaaag gttattagca tgcctaaagg taaagtacaa tttgagcatg ttaaatttgg
    26161 atatagtgat gattttattc ttatgaaaga tattaatatc aatctaaatg caggagataa
    26221 gattgcaatt gttggaccta caggagctgg aaagacaact cttgtaaatt tacttatgag
    26281 attttatgag atacaaggtg gaagaattac tattgatggt gttaatataa aagatttaaa
    26341 gcgtggagaa cttagaacta tgtttggtat ggttcttcaa gatacatggc tatttaatgg
    26401 aagtataaaa gaaaatattg catatagtaa gtcagatgcc acaatggatg aaattgtaag
    26461 agcagcaaaa tcagcaagag tagaccattt tataaggact ttaccacaag ggtatgacac
    26521 tatattgact gaggatgctt ctaatctatc tcaaggtcaa aagcaacttc taacaattgc
    26581 cagggcaata ctttcagacc catcagtatt gattcttgat gaagcaacat ctagtgtaga
    26641 tactagaaca gaggtagaaa ttcaaaaggc aatgaataac ttgatgaagg gaagaactag
    26701 ttttgttatt gcacatagat tatcaactat tcgagatgct gatttaatat tagtaatgaa
    26761 agaaggaact attattgaac aaggaagcca taaggaactt attgaaaaga aaggattcta
    26821 tgaagaactt tataacagtc aatttacaag tgaatatgat gaagatgttg tatgattatc
    26881 aagttttagg caactaaatg attgataaat attaagttaa atatagtaac atgtaaggtt
    26941 atatggaata agagtatttc tctatttcat ataacctttt ttatttatca aaataagata
    27001 aaatttcctt tctaaattag attgaagaat gtatttatca atataaaagt aaaataatca
    27061 ttattattag tgaagtaaat attgtttgcc acatatatgc cataaagatg cacttatttt
    27121 acggagattt aacaaagtag tgtaaagtat actctaaata taaaataaat caaatagagt
    27181 taaataaatt gagaggtgat ataaatagtg aataaaaaat tgaactatag atttgaaatg
    27241 aagcacaaaa taactgaagc agatgtgtta gctttaaaat caagactcta tccaataatg
    27301 aagaaagatg aaaatgcatc taaagatgga aaatatctta ttaggagttt atattttgat
    27361 actcctgaag ataaagcact tttggataaa ttaaatggcg ttgcaataag agaaaagttt
    27421 aggataagat tttataataa tgactgctct tatataaggt tagagaaaaa aatcaaacat
    27481 tacaacatga catcaaagct atcaactaat ttaaccaaaa aagaagtaat aaatatacta
    27541 aataatgata ttgaatttct aaaagagtcg acaaattgtt tattaagaga attttatttg
    27601 aaattaaaat gtgaaagatt agaggcaaaa agcatagttg attacaatag agaagctttt
    27661 atttatccag ttggaaatgt aagagttaca atcgatagtg atattaaaac ttctgtaaat
    27721 tcggtagatt tatttaataa agatttgcca actgtttcgg taattgatga gaatatgact
    27781 gtattggagg ttaaatatga tgaatttata ccagatttta taaaggattt aattcaaatt
    27841 aataagacta cctctacagc agtttcaaag tatgcttcaa gtagattata tatttaagaa
    27901 taaaggctat gatttatata aagatataca taaataagat taaaaaacaa ataagttaaa
    27961 aataaaaaat aaataagtta aaaataggaa ggatgatttt aattatgaca tttaatgata
    28021 tttttaaatc aagttttata gaaaacgtat caggtttttc atttgtggac agtgctttag
    28081 cactaggttc agctttttta gtaggtttgt ttatatatat ggtctataaa aagacttata
    28141 tgggaattat gtattctcga ccattcaatg tgtcgcttgt agcacttaca atgcttacaa
    28201 cttttgtaat attggcagtt acttcaaatg tagttttatc cttaggtatg gttggtgctt
    28261 tatctatagt aagatttaga acagcaatta aagacccaat ggatttagta tttctatttt
    28321 ggtcattggg ttcaggtata gttttaggag caggtttgat tcccttagct gtcatgggtt
    28381 caataattat gggattgatt ctaatattct tttcaaataa aacaatatct gaaactccat
    28441 atatattgat ggtaaattgt aataatgaag attcagaaga tatagcaact gataaaatta
    28501 aaaaagtatt tactaaatat caaataaaat caaaaagtgt tacaccagaa aaaggaatag
    28561 aattagtgtt tgaagtaaga atgaaagatg gagaaacaag tcttataaat gacttgagtc
    28621 aagtagatgg agttactaat gcagttcttg taagttataa tggtgactat gtagcataga
    28681 gaggagtaat cttttatgaa agataaaaaa tttacccttc ttatctcgat tatgattata
    28741 tttttatgtg ctgtagttgg agtttatagt acatctagca acaaaagtgt tgatttatat
    28801 agtgatgtat atattgaaaa atattttaac agagacaagg ttatggaagt taatatagag
    28861 atagatgaaa gtgacttgaa ggatatgaat gaaaatgcta taaaagaaga atttaaggtt
    28921 gcaaaagtaa ctgtagatgg agatacatat ggaaacgtag gtataagaac taaaggaaat
    28981 tcaagcctta tatctgtagc aaatagtgat agtgatagat acagctataa gattaatttt
    29041 gataagtata atactagtca aagtatggaa gggcttactc aattaaatct taataactgt
    29101 tactctgacc catcttatat gagagagttt ttaacatata gtatttgcga ggaaatggga
    29161 ttagcgactc cagaatttgc atatgctaaa gtctctataa atggcgaata tcatggtttg
    29221 tatttggcag tagaaggatt aaaagagtct tatcttgaaa ataattttgg taatgtaact
    29281 ggagacttat ataagtcaga tgaaggaagc tcgttgcaat ataaaggaga tgacccagaa
    29341 agttactcaa acttaatcgt tgaaagtgat aaaaagacag ctgattggtc taaaattaca
    29401 aaactattaa aatctttgga tacaggtgaa gatattgaaa aatatcttga tgtagattct
    29461 gtccttaaaa atatagcaat aaatacagct ttattaaacc ttgatagcta tcaagggagt
    29521 tttgcccata actattattt atatgagcaa gatggagtat tttctatgtt accatgggat
    29581 tttaatatgt catttggtgg atttagtggt tttggtggag gtagtcaatc tatagcaatt
    29641 gatgaaccta cgacaggtaa tttagaagac agacctctca tatcctcgtt attaaaaaat
    29701 gagacataca aaacaaaata ccataaatat ctggaagaga tagtaacaaa atacctagat
    29761 tcagactatt tagagaatat gacaacaaaa ttgcatgaca tgatagcatc atatgtaaaa
    29821 gaagacccaa cagcatttta tacttatgaa gaatttgaaa aaaatataac atcttcaatt
    29881 gaagattcta gtgataataa gggatttggt aataaagggt ttgacaacaa taactctaat
    29941 aacagtgatt ctaataataa ttctaatagt gaaaataagc actctggaaa tcaaagtgat
    30001 gaaaaagaag ttaatgctga attaacatca agcgtagtca aagctaatac agataatgaa
    30061 aataaaaata aaactacaaa tgatagtgaa agtaagaata atacagataa agataaaagt
    30121 ggaaatgata ataatcaaaa gctagaaggt cctatgggta aaggaggtaa gtcaatacca
    30181 ggggttttgg aagttgcaga agatatgagt aaaactataa aatctcaatt aagtggagaa
    30241 acttcttcga caaagcaaaa ctctggtgat gaaagttcaa gtggaattaa aggtagtgaa
    30301 aagtttgatg aggatatgag tggtatgcca gaaccaccgg agggaatgga tggtaaaatg
    30361 ccaccaggaa tgggtaatat ggataaggga gatatgaatg gtaaaaatgg caatatgaat
    30421 atggatagaa atcaagataa tccaagagaa gctggaggtt ttggcaatag aggaggaggc
    30481 tctgtgagta aaacaacaac atacttcaaa ttaattttag gtggagcttc aatgataata
    30541 atgtcgatta tgttagttgg tgtatcaagg gtaaagagaa gaagatttat aaagtcaaaa
    30601 taagattaaa attgaatata aataatttta atactgttaa gtctaaatat ttacttagca
    30661 gtatttttta gaaaataatg tatttggtta ctaatataag gtaataaatt gataaaaatt
    30721 aaatacagca tattaataat acataataaa gtaataaaaa aactgtttaa gtataataac
    30781 ttatatttag acagtttttt tcatttataa ataatataaa tgaaattgaa aacatttgaa
    30841 ggatagaaat tggttggata aatgtataaa ttacttcacc aaaaaataat gacaaaaatt
    30901 gtaactaagt tgtaataaaa tggtagttat ttagcataaa ataatgtatc atagaagtat
    30961 attataaaat taataaaata agatttttgt aagaaaactg ctggcttttg atatttagag
    31021 tgagtagtta aattatgttt aggaggggta gaatttgttg caaaaagtat tatggaaatt
    31081 tttaaagcta gtagtcggtt tatttatatg ttcagttggt atagtattga cgataaattg
    31141 taaccttgga ttatctccct gggatgtatt tcatcaagga ctttcaaatc atataggaat
    31201 aactatagga actgcttcta taattgtagg ctctatagta gttattgctg atgtcgttct
    31261 tggagaaaat gtaggatggg gaactgtttt taacatgcta ctaattggat tttttatgga
    31321 tttactttta tatagtaatt taattccaga ggctgataat ttatttgtag gaataattat
    31381 gttgatatta ggattggttc ttttgtctgt tggaatggta ttttatatgg gaagtggttt
    31441 aggaagtgga cctagagatg gtttgatggt tgcaattcaa aagaaaacag gaaaatcact
    31501 taaattaata agaggtacta tagaagttgg agcattaata gtaggatttt tattaggtgg
    31561 aaaagttggt ataggaacta taataagtgc atttggattg gggtatttca cacaaatggt
    31621 atttggctta tttaaattag attgtagtaa gattaaacat agatttatag tagatgatat
    31681 aaaatttatc aaagtgtata tgagtggtga gaaaaaagct gctgttcaaa atatagttaa
    31741 gtaatttaag aataaattca acgaaatgtt aagtatttta aattaacatt ttgtttagat
    31801 agataaattg aatattaaaa gaatattaaa atatgaggat tgctagaaca ctataaaata
    31861 tagatttcta gtaatttttt atttgttcaa tattttttta gtactagaag tcttattctc
    31921 tatttaatga atgaattaat ttataattag agatactaaa ctttatagat attaatataa
    31981 acacctttaa actgtgtatt ttccaaagcc tttaaaatgc ttgtatttat tctaaataaa
    32041 aaaataaaaa aatgtcaaaa aaactcttgt attatatcca aattttatat ataattacct
    32101 atgttgttta gaaattttaa actcaatgtt tagtaaacaa aatgtttagt aaaataaaac
    32161 agaaactagg aggtgcaaca tggatatagg tgaaaagata aaaagattaa gaacagaaaa
    32221 acaactgacc caggaagagt tagctaatag atgtgaattg tcaaagggat ttatttctca
    32281 attagaaaat aacttaacat caccatccat agctaccctt atagatatac ttgaaatact
    32341 aggtacaaat ttaagggaat tttttaatga aatagatgat gaaagaatca gttttacaaa
    32401 agaagatatg tttgaaactg aagatgaaga tttaaaatat aaattaaagt ggttaattcc
    32461 aaattcacaa aaaaatgaaa tggagccaat aataataaca ttgtatccag gaggacagta
    32521 taaagaagaa aagccacatg aaggtgaaga atttggatat gtattagcag gttcaatata
    32581 tgtccatata ggcgaaaaga aaaataaagt aaaaaaagga gagagttttt actttagacc
    32641 gaaagcaaat cactatatat caaacgaggg caaaactaca gcaaaagtga tttgggtaag
    32701 tacgccacca tcattttaga atagaggtgt ttaataattg gtagaaaata taatagaatt
    32761 aagggatata tctaaacatt atgaagaact tactgtttta gataatttca atttagatat
    32821 aaagaaaaat gagtttctaa cattgcttgg accaagtgga tgtggaaaaa ctacaacatt
    32881 aaaaataata gcaggatttg agtatgctga tgatggtaaa gttttatttg aaggtaagga
    32941 gataaataat ttgccaccat atgaaagaca agtaaacaca gtatttcaaa aatatgcttt
    33001 atttccacac atggatgttt atgaaaatgt agcttttgga ttaaaaatta aaaagactcc
    33061 aaaagatgta atagatgcta aagtaaaaga ggttttgaaa cttgttgcac tagaaggttt
    33121 tgaaagacga caagttgaat cattgagtgg aggacaacaa caaagaattg caatagcaag
    33181 agctctagtc aatgaaccaa aggtattatt actagatgaa ccacttggag cacttgattt
    33241 aaagttaaga caagagatgc aaatagaatt aaagagaatc caaaaaaaat taggaataac
    33301 atttgttttt gtaactcatg accaagaaga ggctcttact atgtcagata ctattgttgt
    33361 aatgtataag ggcaaaattc aacaaatggg aacaccacaa gatatataca atgaacctaa
    33421 aaattcattt gtagctaagt ttataggtga aagtaacata tttgatggaa ttatgataga
    33481 agattataaa gttaatttct gtaatagaga ttttgagtgt gttgataaag gattcgaaaa
    33541 aaacgaaaat atagaagtag taataagacc agaagatata aaaatggtaa agccagaaga
    33601 aggaatgtta aaggggaaag ttacatctac agtgtttaaa ggagtgcatt acgagataga
    33661 attgaatgaa aatggaagat tgtggatact tcataatact aaaaatgctg aggttgggac
    33721 tgaactaggc atggatatat atccagaaga tatccatata atgagaaaag agaataacta
    33781 agatgaaaag aaaatctttt ttagcatatc catatgtagt ttggagtgcg atatttgtaa
    33841 taattccttt aatattagta gtatttttta gttttacaaa agaatcaggt ggaggttatg
    33901 cctttacact tgagaattat aaagaagtaa ttgaccctat atatatgaaa gtttttgggc
    33961 gttctattct tttagcagga ggagctacat taatatgcct tatagttggt tatccagtgg
    34021 catatataat ttcaaaggct cgtgtaagta gaagaggttc attaatatta ttgtttattc
    34081 ttcctatgtg gatgaacttt cttttaagaa catatgcatg ggttgctata ttaggaaaaa
    34141 atggactttt aaatacattt ttaggttggt ttggaataca accattagca attttgtata
    34201 ctaattttgc tatactttta ggtatggtgt ataacttttt accatttatg gttcttccaa
    34261 tatatacagc actatcaaag atggataatg atttgataaa cgctgctcat gacttgggtg
    34321 caaataatat gacagttttt agaaaaatta tatttccttt gagtttgcca ggtgtaatgt
    34381 ctggaataac tatggtattt atgccagcag tcactacttt tgcaatatca agactgttag
    34441 gtggaggtaa aataatgctg gttggagatt tgattgaaca acagtttaca gttgttggag
    34501 attggaactt tggttcagct atatcgatat ttatgatgat agtaatatta atatctatgt
    34561 ctataatgtc aaaatttgga gatgaatctg acaaagaagg cggtgggtta ctattttaaa
    34621 attaaagaaa ataacttcta atatatattt agcattagta ttcttatttt tgtatgcacc
    34681 catatttgct ttggtactat tttctttcaa tgattcaaaa tcaatggcaa gatggaatgg
    34741 atttacatgg aagtggtatg gtcaattact tcaaaatgag agtataatga gtgctttata
    34801 ttatactatt gtaatagcta tattagcgtc tgtaatatct acaatagtag gaactataag
    34861 tgctatagga atacataaga tgagaggtaa gagtaagaaa ttaatactaa atgtaaacta
    34921 tcttcctatt ttaaatacag agatagttac agcagtagct cttatgagtc tgtttgtatt
    34981 tgtaaaaatg gaatttggat ttacaacaat gctattggca catataatgt tttgtttgcc
    35041 atacgttata ctttcagttt tgccaaaaat gaagcaatta cctgataata tagaagatgc
    35101 agctatggac ttgggagcaa cacctatata cgctcttaga aaagtaattt taccacaaat
    35161 aaaaccaggt atagtttctg gatttttaat tgcatttact atgtcaatag atgactttat
    35221 aataagtttc tttaatgctg gaaatggtgt aagcaatctt tctatagaaa tctatggaat
    35281 ggctagaaga ggtataaaac ctgaaataaa tgcattatca acaataatgt ttgcagtggt
    35341 tctaggttta cttttactag ctaataaaaa agaatctata gtaaggggga ttaaataatg
    35401 attaagttta aaaaagcaat atcccttata gctgtaggta tgatgacagt tactttattt
    35461 acaggatgta ataaagcaac tgattctaca aaagttttaa atgtatataa tgttggagat
    35521 tatatagatg agtctcttat agataagttt gaaaaagaaa caggaataga tgttcaatat
    35581 tcaacttatg atactaatga aatgatgtat caaaaagtaa agagtggtag tacacactat
    35641 gatttagtat ttccttcaga ctatatggta gagaaaatga aaaatgaagg tcttttggaa
    35701 aaattagatt ttaagaatat acctaatatg aaatatttag ataagagttt tttaaatcct
    35761 gtatacgaca aaactaatga atatagtgtt gcatatatgt ggggaactct tggaatattg
    35821 tacaataaga aagaagttaa agaccctatg gatagctgga atatactttg gaatccaaag
    35881 tataagggaa atataatgat gtttgactct gttagagata ctatagggat aactcttaaa
    35941 aaattaggat atagcatgaa tagtgtaaat ccaaaagaaa taaatgaagc taaaaatctt
    36001 ttaatgaaac aaaaggattt agtattggct tatgtaaatg atgaaggtaa ggatagattg
    36061 cttggtggag aagtggcaat gggtatgctg tattctggag atgcagttac actaatggag
    36121 caaaatcctg accttgatta tgctatacct aaagagggaa ctaataaatg ggtagatgct
    36181 atgtgtgtac ctacaacagc acaaaataag aaagaagctg agctgtttat aaacttttta
    36241 ttagacccag agaatgcaaa agtaaatgct gagtacattg ggtattcaac acctaataca
    36301 ggagcactaa aattattaga cccagaaata actcagaatc ctgttgcata tccatctaaa
    36361 gaagtacttg ataaatgtga aacttttgaa gatattggtg aaaatataaa attatatgac
    36421 aaggcttgga tagagttaaa atcaaaatag ggtcttgttg ttttaagtaa atttacttaa
    36481 taaatactta ataaaactgt tcttttttag aaaatttatt ttctaattat tgaacagttt
    36541 tattttttgt ttaagttata ttcatatgca atttaaatag tttattaata aatgaaaact
    36601 tatttgtata atgactttaa taataattag aaaaaatggg caacttaata gaataattgg
    36661 aggtgtaaat ttataaaaaa tgaataggat aaataaaaaa atagatttat taatattggc
    36721 tttgctcgtt tttatatttt taattgttgg aatagtaata ctatcgatta ggacagaaaa
    36781 taaattgaat gtgttcataa tgttagcaat aatttttttt ataataatgt taacttattt
    36841 atcaaattca gtagtggggt tgataacaag ttcaataatt atatttatgt atacatccta
    36901 tattttatat aacaatatta ctcacaatat ggatgttgaa tttatatcat atatgtggat
    36961 aatagcaaca ccagtatctt caattataat gggtaatctg aacaaaagca taaatgaatt
    37021 gcaaaataca aataaaaaac tatctgaaca gtataaggaa ttagtgacta ttgatagtga
    37081 aactggactt agaaatttaa agatttttta taatgatgta aatatggaga taagtaaatc
    37141 tattagacat aatactgatt tttctcttat gatagtaaaa ttaccatact acggaaacct
    37201 acagactata tttggtgaaa acaaaactaa taaaatagta aaatatattg gttcaaatat
    37261 tatagaatgt acaagaaatg aagatataat ttatagttta caaaaagata tgatagggat
    37321 actaatgcca aatacatctt tggaaggttc aaaagttgtt aaagacagaa taaagaaaag
    37381 gataaaagaa ttaaatttag atttaaacaa taggggaaaa tatgtaaata tagatgtcaa
    37441 aattgcattt ttacaatata agagtagttt tggagatagt ataaatttta agaatatagt
    37501 tgaagaggaa cttcaatatg atgtttaaaa tatttaataa aagtctatta aaattagtag
    37561 ttgtattatc aatagtactg acaatatcaa attttagctt atcttcatat gcatcagaaa
    37621 ttacttccag cacagataag gtattaatca tatatgattc taagaaagaa acagcttata
    37681 atagagatat tttaaatatt atgaggactc tattgggaag atttagttca gatatagagc
    37741 tattaaaatt gagtaactat gatggtgaga taaataaaaa ttattacagt catatattta
    37801 ttttaggaat caatgaaaac tcatataata atgataaaaa cactaaaaac ttgattagtt
    37861 ccttaaattc atataaagga actatatgtt ggttaggata tggtatagaa aatcttttgg
    37921 aacataaaaa atacaattta gactatgtag gaaaaacaaa caatatagtt agtgtaaatt
    37981 atagaggtaa atcatataac ttggatgaac attatgtttt taacattgta gaatcaaaag
    38041 atacaagtaa taaagtaata ggaagtatta atgatacctt aaataagtat ccatatataa
    38101 taaatgataa aaatcttttt tatgtatcaa aattagattt agatggagtt ttattttata
    38161 tattttgcga ttcacttaat gatattttta atattaagac gtttgataaa ggacgtatat
    38221 ttgtaagaat agaagatgta catgctttta gagaaccaaa aaatcttgta gagatagcgg
    38281 attatttaag tagtaagaat ataccattta cgattgcatt aataccagca tatgtaaatc
    38341 ctaaaaatca taaggtaata actttatcag aaagccctga aattgtcaaa gcaataaaat
    38401 atatgcaaga taaaggtgga actgtgatat tgcatggata tactcatcaa tacaagaaag
    38461 aagaagtttc tggagaaggg tatgaatttt gggatggtaa aaaagatgaa cccttaaaag
    38521 aaaatatgaa gatatttgtc aaagatagag ttttgaatgg tcttagagta tgtatagaga
    38581 atggaattta tcctcttgca tttgaagcgc cacattatgc aatggaatca gagggatata
    38641 aagaattaaa aaaatatttt tctacttata tagggcaaca tcaaaataat gataaaaaat
    38701 ttagcacaaa tacatatcct tatattatta gggatacaga ggagtttaat atatttatac
    38761 ccgaaaattt aggatatata gacccagaag ataagtttac ttttcaaaat ataaaagaaa
    38821 atttagataa attatccatt gtaagaggtt ttagtggagg gttcttcttt cattcatatt
    38881 taaacataga gtatctaaaa aacacaatag aatatttaga aaaacagaat atagaattta
    38941 tgaatttaag agattttaat aattgggtaa aagttgatga aatacaaata agaaataatg
    39001 gagatgaaat tatagtcaac tatgataagg acttagatga actaactaaa agtgatacta
    39061 gatttaaatc catatccaat attagtaaag ttttaatatt catagtatct ataagtgttt
    39121 taatatttgt gattatattt atatatttta agagaataga taaaaagaaa tttttaaaat
    39181 aaaggatttt atattagaat caatatttta aaatatttta ggagaaattt atgagagatt
    39241 taaatgtatc agagtatttg ttcgtattta gtttgtttag tatatggtct ttattgctta
    39301 taaatattat tttagctatg ggtggatata tattttattt taaaaacttt gataaagaaa
    39361 taaaagaaat tgatgaatat cctatgatat caatacttgt tcctgctcat aatgaagcta
    39421 aagtgatagg tagaactgtg gagtctttgc tattactcaa ttatcctaag agtaagatgg
    39481 aacttattgt tataaatgac aattcttctg ataatagtaa agaaatttta gaaaatataa
    39541 aggataggta caacaattat aatttcacta taataaatac agatagttta acaggtggaa
    39601 aaggaaagtc taatgcatta aatattggat atacgatttc aaaaggagat tttatagctg
    39661 tttatgatgc agataacaca ccagataaaa atgcacttag atatctagta caaactatag
    39721 taatgaatga tgaattaggt gctgttattg ggaaatttag gactcgaaat aaaaataaaa
    39781 acctattaac caaatttata aatatagaaa cattaagctt tcaatggatg tcacaagctg
    39841 gtagatggca actttttaat ctatgtacaa taccaggaac taactttata ttgaggagaa
    39901 gtatcataga agaaattgga ggatgggata gtaaagcaat tgcagaagat acagaaataa
    39961 gttttaggat atataaattg ggatacaaga taaagcttgt accacaatct ataacatggg
    40021 aacaagagcc agaaactgtt aaggtgtgga taaagcaacg tactagatgg gcaaaaggga
    40081 atatatatgt attaatgaaa tatataaaaa atatatttaa acagggaaga aataagattg
    40141 tgtttgatat agcttacttt ttttctgtat attttctatt tttaacatct gtaattatat
    40201 ccgatatttt atttgtttta agtatatcta aactagtaga aatttctatt ccaattaact
    40261 tttttctaat atggatacta tcttatcttt tatttattat agaagtgagt ataagcctta
    40321 ctatagaaaa aggtgaagca acaattgaaa atatattcat agtggcaata atgtatttta
    40381 catactctca actatggctg tttgtagcta taaaaggaat gatagagtat ctaaaagata
    40441 taatttttaa aagagaggtt aaatggtata aaacagaaag attttgattc atgtacagga
    40501 ggaattaaca tgaaaaaatt tattatatca attatatctt tagtattgtt tttttcaaat
    40561 atatcactta tatacaaagt aaatgctgat gagactaaag ttaaaaatta taagtttgaa
    40621 agagatataa ctattgatgg tgttatagga agtaatagta ctttttttga agtaaataaa
    40681 aattgggata ttgaagaagt tttattgcat cttaatttta gtaagagcca aatattaaat
    40741 ggagatgttt caagtttaac tgtattgata aataatgtac caataaaatc tattaaatta
    40801 aatgctaaga caaattataa aaatacatta gaagtattag ttccaaaaga ttatataatt
    40861 caaggatata atgaaataaa aataaaaaca tacaaaacaa tatctgataa aatttgccaa
    40921 gatgattcca atactgggaa ctggatggtt attcacaaag aatcatatat atcaattaga
    40981 tataagcaga aaaaggtgga aaactcaata aatgaatatc cgtatccata tgcagaaatt
    41041 gaaaataatc ataaattaga tacaactata gtagtaccag ataatatgac tagaggagag
    41101 actacagcag tgtttaattt ggcatcagca tttggaaaaa taacaaaaaa tgatgattta
    41161 aagttagatg ttaaattata ttcagaaatg aaaaattggt cagatgataa tattatatat
    41221 attggtaaac ctgaaaatac agcagaagaa attttggata ttttaagtat aaaggaacaa
    41281 accctattat cttcaaactg tataatcaag caagtagatt caccatacaa taagaataag
    41341 aagatgatgg tggtaattgg tagtaatgaa gatgatttaa taaaggcatc aaatttatta
    41401 atagaaaata gactttctaa ccaagtttta tcatcatcag tacttgtaaa caaagagact
    41461 aatataaaaa ttaataggga acaaaaatta aatttaggtc atttaacatt aaaagattta
    41521 ggatattctg actttttgtt ggaaggagca tttaatcaac aagctttgtt tgatgtcaaa
    41581 ataccaacgg gtaaggtatt agatgatggt tcaaagatta tcttaaattt aagatattct
    41641 gacaatttag attttgaaaa atctttagtt acagtgtcta taaatgatgt tattgtggga
    41701 agtaaaaaat tggataggtc acattctaat aatgataagt tagaattaaa aatacctaaa
    41761 gatatagata ataaaaacta ttatcaagtt aaattgacat ttaatttgag tataaaaaat
    41821 tcgaattgtg ttactagaga gagtaataat ccttgggcat atgtatcaaa taattcttat
    41881 ttggcgttat ctacgaaaga aaatgaaacc ttatcttttg aaaattatcc gtatccattt
    41941 gttagagatg atgaatttaa tgatttaact gtgataatgc cagattattc agggtcacaa
    42001 gctatgacct ggatgtttag attaggtgtt actttaggtg cgaatataaa ttcacataat
    42061 ggaaatataa atgtaattag aggaaaagaa tttagtgaca agtataagga tactaatatt
    42121 gtagtttttg gagtgccaca taataattct gttataaaga tgttaaataa taatttaaat
    42181 ataaagttcg ataaaaatta ctccaatttt atatcaaatg ataagataag ctttattgat
    42241 gattatggaa aaaatatttc tactattcaa ttaataaaat caccatataa taatcaaaaa
    42301 aatatcatgg taattagttc aatgaatgaa aaaaacttat atttaggaat ggattatttg
    42361 ttgaataaaa gtaaagttaa tgatttaaaa ggagatacat tgataattga tgaatacgga
    42421 gaggtagaag atttagctta taatttaaaa tctaagaaag aagtaaaaga ttcaagttgg
    42481 aatatgagta taaataagac aactaaagta tttttaatga tttcttttat tactataatt
    42541 atagtcatga tattatcaat gctatatata aaaaaatata aaagaagata atatgataaa
    42601 tttattattt taaaagaggt atataatttg aggaaatata tgaatgaatt taaattaaaa
    42661 agaagtattt taatctcatt aataatctta atttttatat ttatagttta ctttgtaagt
    42721 attgagttaa aagataggag tctagtaaat aatcaatata aaggtaagat taaatcggcc
    42781 aatttatctg tagattatga aataaatcaa gttatgaagg atatagataa attgggattg
    42841 aatacagtaa atgtacctat aattataaat gtagagtcca ttaattctga tattatgtca
    42901 atagataata atagtaaaga aaaggcaatt aagttaataa aaaaattaaa taaaaaaggt
    42961 atttcaacaa tattagaggc atatccatgg attgaaaatg gaaaattgta tgagactgac
    43021 tggaacccta taaataaaaa aagatttttt tatacttggc aaaatgccat attaaatgaa
    43081 cttataattg atgtagcaaa tccactggat gtaaatgttt taaatattgg ttctaatttt
    43141 gtacatttgg aagaatatca acaaaattgg ggtgaaataa tagattttgt acaatccaaa
    43201 tttgacggtc ttgtaacgta cagaactaat tggtggtata caaaaaaaga tgatttaagt
    43261 agtaagttgt tttatgaaca aaagctgaac aataattttt ttgataagtt agattttata
    43321 tctatagcag catattttga gttaagtaat aaacctgtta atacagtgga tgaattaatt
    43381 tctgccttac actcatctac tgtaaacaat agaggacaaa atataaaaca agaaatatat
    43441 aacctttata aagctcatag aaaacctata ttttttggag aattaggttt ttcaaataga
    43501 gaaagtgcaa gttctcagcc gtggaatcat actccttcaa aagctgtaaa tggcgaagaa
    43561 caagcaaggt gttttgaggc atataagaaa gtttttgaaa atgaagactg gataaatggt
    43621 ttttctgtgt tttgtgtagg taaaatagat gacgaaaaaa atttttaccc atcaaaagag
    43681 agtataaaag ttattaagtc ttggtatgaa taattttgaa agtgtagaat ttatatagat
    43741 taaatagatg attaggagag atataaatga atgacatcaa ggttatgact gtatttggaa
    43801 ctagacctga agctatcaaa gtagcacctt taataaagga acttgaaaaa agagagaata
    43861 taaaaagtat tgtttgcgtt acagctcaac atagagaaat gcttgaccaa gttatagaaa
    43921 cctttaatat aaatgtagac tatgatttgg atataatgga aaaaggacaa agcctgaatg
    43981 atataacatg taagatttta aataaacttc ccttgatttt aaacaaggaa aatccaaata
    44041 taatattggt acatggagat acaaccacaa ctctagctac aagtttgact gcattttata
    44101 ataaaactct tgttggtcat atagaagctg ggcttagaac ctatgataaa tattctcctt
    44161 ttccagagga gctaaataga caacttacag gtattattgc tgatatgcac tttgctccta
    44221 ctaatttggc tagaaaaaac ttgatttctg aaggaaaacc taataataat atatttgtaa
    44281 ctggtaatac tgctattgat gctttaaaga tgacaataaa agaaaactat aatcatccta
    44341 taattgatga gattggtaac gataggatga ttttgttaac ctctcacaga agagaaaatt
    44401 tgggtaaacc tatgaaaaat atattcagag ctataaagcg aatagtagat gactttgaag
    44461 atgtacagat agtatatccc atacatttaa atcctaaaat acgaactata gctgatgaga
    44521 tatttggaaa atttcctgaa aaaattcata ttatagagcc tttagatgta gctgattttc
    44581 ataacttttt aaataaatct tatatgataa tgactgatag tggaggaata caggaagaag
    44641 ctccatcatt aggtaagcca gttcttgtgt tgcgtgataa aacagaaaga acagaaggaa
    44701 ttgaagcaaa gacacttaaa ttagtaggta ctaatgaaga tagaatatat aattcagtga
    44761 gcgatttatt aataaataaa gataactatg ttcagatgag taaagcatct aatccatatg
    44821 gagatggaaa tgcaagcaaa tatatagtgg atataataat taaaaaattt aattgcaaat
    44881 atttaaatta gataaggcct aaaacattaa ctaattgata taagatttat gataatatta
    44941 agtaatacaa tttttaaggg ggagtaatat ggctagaaaa ttaataaaaa atttgggtaa
    45001 aagtaaaagt gtaaagagag tgaaactttt atttaagaag atttttatta ctgtattcat
    45061 agtagcaagt atagttgcta tttttaatat aacgaaatat tttgaggaat tatacaaagt
    45121 aagggattta aaaagtacta aaattgaata ctatatggat gtggctgatg aagcaggaga
    45181 tggaaaggtc caattaagtt ggaaggcctt acttgctata gacatggtaa tacatgatga
    45241 agatttaagt aatataaaaa agaaagacac attggatata ggggaaaagt ttatagtaga
    45301 agataaaaat ggtaaaggcg aaaaggtgta taaggtaaaa aagtttaata aggtattaag
    45361 cgaattgaaa tttgactctt ctcaaaaaag tagagcaaga aaatacatga aagatttaga
    45421 atacacatac cttggaaata aacaattaga tagtagtgat gaaaaaatta aatttataaa
    45481 gaagttagaa gactcagcta taagagaata tattgattat ggaatattgc cctctataac
    45541 aattggacaa gctatattag aatctggttg gggaaattct aaacttacaa aacagagtaa
    45601 taatttattt ggtataaaag cagataaagc atggaaagga aaaagtgtag aaatttcaac
    45661 ttcagagcat tataatgaaa aaattgtagc tagttttagg tcgtataatt cattacaaga
    45721 ttctgtcaaa gaccacagtt tatttttaat taataataaa agatatagaa aacatgggtt
    45781 gtttgaggca aaagattata ttagtcaagc tcaagcatta gaaaatgcag gatatagtac
    45841 agcagaagat aaaaaaggaa atcgtatata tgcagagcta ttaatagatg ttattaggag
    45901 ttataatttg caattgatag ataataaagt tgagacaaaa taattgttaa ctttatatga
    45961 ggagataaca agataagatt tttttctaca aaaagaatag aaaaattgta aaaaaaacta
    46021 aaaaaatgta ttgacttgtt atttaaaagg tgatattatt attactgtac tcgaaagaga
    46081 acaaaa
//
LOCUS       DAGSRM010000025        43824 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.025, whole genome shotgun sequence.
ACCESSION   DAGSRM010000025 DAGSRM010000000
VERSION     DAGSRM010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43824
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.025"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            439..1068
                     /locus_tag="MJE33_001487"
     CDS             439..1068
                     /locus_tag="MJE33_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY9647428.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRAVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAQTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
     gene            complement(1182..2696)
                     /gene="glpK"
                     /locus_tag="MJE33_001488"
     CDS             complement(1182..2696)
                     /gene="glpK"
                     /locus_tag="MJE33_001488"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBY9647429.1"
                     /translation="MEKKYVMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDEDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASVQWLRDELKIIESAKDSEMYAN
                     RVEDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVDIDRPKIVETTALGAAYLAGLS
                     VGFYIGRNEITSKWSVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            2940..3482
                     /locus_tag="MJE33_001489"
     CDS             2940..3482
                     /locus_tag="MJE33_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY9647430.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSNISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLNIIFTEIRNFINKVHNIQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKMFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            3496..5988
                     /locus_tag="MJE33_001490"
     CDS             3496..5988
                     /locus_tag="MJE33_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY9647431.1"
                     /translation="MNHNTSIKNKDSNPKIFLVLTLYLLGIFMGAIDTGIVSPARTII
                     QNSLGVNEKTGIWMITIYTLTYASVIPISGKLADKIGRKYVYLVSIFLFGSGSLICGL
                     SSLFSNFYILLIGRVIQAIGGGGIMPIATAEFGTTFPENKRGMALGLVGATYGIANIL
                     GSSIGSTILSIFGTQNWKWLFFVNLPICLIILIGGVFCIKNNKSESKEKIDKLGTLML
                     ICIIVSLLYGLMNIDFFNFKNSIQDISVYPYLLTFIVLIPIFIFIENRAKDPILSFEY
                     FLNPRILIVLILSLIVGIGMMGMVFVPQYAENALKINAGSGGYFVAILGLFAGIAAPL
                     SGRLIDKFGAKKILLLGFSVSMIGSLYLVLIALKTNTVFSVCVSLIFMGLGMGFTMGT
                     PLNYMMLSNTRLEESNSALATLSLIRSIGTSISPAIMIGFIAHAGLSVQDNIMDLVGK
                     PSTPKIVQLEELENMIDDLKSDPEMAKNLKNVSIPNMNDTSNINMDMTGGKLPKSLLN
                     KVQSADTTNITNITKEISTTMFDEKVPSIITKIQKNVQKGIDGTQSGINGIEDGENKL
                     NSGIKGVQTGIDNMVKARAGINQGIDGVKKGIAGVDKGIKGMEQGLEKQDEAIKELTT
                     AYNKIPTTEKTPTDNENNSNEQNQSNTSNSESDDTKNDSTENKNNNSSENKPNVNEQK
                     ESLNAQIQQLKKTRDDLNTKLQKNKTQKNELSKKLRSMESQKKELQSKLNNSISQKKD
                     MEKSLDTMQQQKKSLQAVLAKTQEVKTEIPKAFDKSKLDYINSIENNRTKIENTLQST
                     LNSGFKQMYITVFCVNLLAFIILLFYKENKTR"
     gene            6409..6807
                     /locus_tag="MJE33_001491"
     CDS             6409..6807
                     /locus_tag="MJE33_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY9647432.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     ILMSIADYFNVTTDWLLYGKEKVNVNSSVKEDKEDYLHVTNDEMTILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
     gene            complement(7024..7233)
                     /locus_tag="MJE33_001492"
     CDS             complement(7024..7233)
                     /locus_tag="MJE33_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647433.1"
                     /translation="MCKLDSDGVIECCREIDDFITALSNIKSLNMERLNTLTKYSSTC
                     SILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(7265..7474)
                     /locus_tag="MJE33_001493"
     CDS             complement(7265..7474)
                     /locus_tag="MJE33_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY9647434.1"
                     /translation="MDARKKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            7773..8192
                     /locus_tag="MJE33_001494"
     CDS             7773..8192
                     /locus_tag="MJE33_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY9647435.1"
                     /translation="MDTLGERIVYLRKAKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSIAEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPLNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKEKDLGKIEYNKNKDEKIDK"
     gene            8420..8875
                     /locus_tag="MJE33_001495"
     CDS             8420..8875
                     /locus_tag="MJE33_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647436.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDAKIAILVNRQ"
     gene            complement(9096..9356)
                     /locus_tag="MJE33_001496"
     CDS             complement(9096..9356)
                     /locus_tag="MJE33_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647437.1"
                     /translation="MDKLITELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVQMGIMKTDVEEIKEDVTGIKGMLQNGV"
     gene            complement(9619..9972)
                     /locus_tag="MJE33_001497"
     CDS             complement(9619..9972)
                     /locus_tag="MJE33_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647438.1"
                     /translation="MFKFDKNKIEQIKQGRKVEMQYKDISDISIGQVKQDDDITNNFI
                     ANAEIYEMLLNQSSVNEASNISSFSVRKSGGESGMVEVYVALILRGKKTIEEVPAVIR
                     EQVRIRCKELEIPVE"
     gene            complement(9989..15310)
                     /locus_tag="MJE33_001498"
     CDS             complement(9989..15310)
                     /locus_tag="MJE33_001498"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012632.1,HMM:NF024930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647439.1"
                     /translation="MKRTKLLQRGNFFGDKNMVVDEFDEGYDNYDFINFFTGCCNYTF
                     GLKNNNILYGCGDNSNFQLGLGEDNTTRKLFTKIPNISTNIKKVACGESHAVILTSDG
                     ELLVAGINTDGQMGLGLEKVGKTVSTFEKVPEIKGVKDIACGLQSTYLLYNDGTLYVA
                     GNNLYGQLGLGTNGASANVNTFTKVDVDNVKAVFSYNKSAFIIKNDNKCYSTGFNNQG
                     QLGLGDKNNRDLFSLVSINDVKTIACGSEHTVLMTYNNDIYGCGKEKCFGNALQSSLF
                     TKIEEVNIKTIACGHGNTMLIDNKGTLKVAGNNDIYQLGIANYSENIDNSFIDLKNIV
                     AKNIFIGLSHSILIDSNNDSYCTGDNTYGQLGSFFDDMHIVEFKKMDSEKYSYSNYIN
                     LIKSEDKLTLLKEEMEIKDIELPLDIHSVRDVVFSPYCTLVILGNGDVYGLGNNRYKG
                     MGSDLPSQLNELTKLSISNVKSIVASKNISGGIFYIKNDDTCYYSGPNSNSIAGVLPS
                     NSDIFKKISIDNVKKVVINTDLSNWFSLIVTNNKQIYTSGKSSSYVNGLSNKSASQYT
                     EIGVSNVTDAYSSYNATFIVADEKKVYATGINTNYLLGVNPNDGVNINLTLINNWFYV
                     NPSGASYSTNSCTNDITKINNIIIYEYMAILCTNYGSFLTGYHGTLWTKPSDSTYRVQ
                     QQGITHAGYISNYIYNYYPTRCTQSSYSTNFAYLYYGESTSNLKNVNPDNLLISGGSS
                     YIHQYGRNYLNNQSSNNIAASNINSGPITSDKAIFLYKALLYLSSNTLYGFGNISESA
                     KELDVSDTQDGYNATNYKKVMKNIKNIFIPPYDLSRDKTRFAILTDKSLFICGYNSKG
                     THGISVNSSLNLNNKINYNKKNSSSEISSNIQEIYSHSKSTYLLTNNNMLYSVGLNDV
                     GQLGVGDEINRKVFTKINIDNIKSINVNRFTDNSKHVFAIKNDNTCYAVGLNNSGQLG
                     IGDNVNRNVFTKINVENVKYVAVYGNTSLLLTNDGLLYGAGNNGKGQLGLGDTTSRNI
                     FTRIPINGVRDVYICNDVSIIVKNDNTCYVCGFVNGYFGFTEGSISTFTKINIENVKS
                     VVTAGSEATFFITNDNVIYTTGKKERVFFSTETNDIKGIRVINNIINAKKIVVNGYTS
                     AILTNDNKLFVGGLSGYGSIANNNNTNSVEDVKDVFVTANNTLYIDNNNNLISSGRDT
                     YGISDESYRDMSVPYYKVSIKKDVDTVFSSYNTIFIKDIYGKFYSSTGDNRYNHLGIH
                     HRYDNDKNEALEGSLHSYFKTDNTSDKIVFNKKNEKLVMFNDKYIKTNNKYINYKNIF
                     KDNFKYTSIILPFEVSDIDISKTHSLAVAKDGKLYGIGSNSYKEINQTLEDIELLTLT
                     EVNISDVKKVACGDNYSYIIKTDNTLWSYGKNTEYQLGVGHNNDVRELQKVTGLPSVK
                     DISIYNSMTLVLTNEGELYAQGYNTNGLFGLGESEKDKIIRTFTKVLTNVKEIKSHND
                     DHILVIKNDNSLWITGKNKSMYKISISITDLYEFTKIPIPEHLNDILDIELSDDTIYM
                     ITKVDTSKASIEIVEKSISQVRVVVQDPNNVIEKLEMFINDELISTKTNLEINSIIFE
                     IPQNKIVLGENKILIKASSPTGDLYSSMFIFKSETGLKVKKDSILMINNKVYSIINIT
                     ENNTDLIVTLNEGLKDDMMENNPIYQLINKTKVQVKINKSDLFKDMKLVEIKKSDSSY
                     QEIYELEEANIKSAQPKIIVEKGDKWTAIKRPSMIFRYDAESNEPQA"
     gene            complement(15327..16310)
                     /locus_tag="MJE33_001499"
     CDS             complement(15327..16310)
                     /locus_tag="MJE33_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBY9647440.1"
                     /translation="MEEKFYIILTKIGREKIANATALGELVGLTKFQVGDSNGEYYEP
                     TEEQTALKNVVWEGNINSLRIDEKNPNWIVIETILPGTVGGFMIREAAVLDNENNIIA
                     IGKYPETYKPRAEDGSIKDLVVKMILQLSNTSNVTLEVDPTLVFVTQKDIQDLDDKFD
                     KNIKEIKVKIGEELLSTEAKNLSGAINEVVEKIKNISIDDVIGGQIQTELSVLKNSYN
                     KLSEKVLDILIYLELESEIDVDEAGYWYDTLTNAKNIIAIEGLKLDLNRKCITGELGS
                     VTFKNVVLPFNANRVRYIHEMDNNFVETKSNRAYSIGQTDITLNKYSYEIR"
     gene            complement(16311..17009)
                     /locus_tag="MJE33_001500"
     CDS             complement(16311..17009)
                     /locus_tag="MJE33_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HBY9647441.1"
                     /translation="MKLIDKLPSFDRNYIVEEIQGAYDTELNILKEDIDDTFNQLFVD
                     TATWGLDMWEDILCIEKKELDFDTRRSNIKAKMRSRGTSTIEVIKSICEAYTKSETDI
                     KVYSDEFTFVLSFIANNCDYKTLLDCSDMIERVKPAHLLHYLEPIILDKSMVYCGGGM
                     VCSEEVKVHPYFEPIIKCSAVVNCGAGMISREEIKVYPLSIKCIENNCKINIAIANDT
                     GVENVVVYPKSEVV"
     gene            complement(17006..18058)
                     /locus_tag="MJE33_001501"
     CDS             complement(17006..18058)
                     /locus_tag="MJE33_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBY9647442.1"
                     /translation="MYSDQTYEVIKNRTLENINLDIYKGEGSFLNNMVSGNNLELSKI
                     YLELSKMHKMAFIQDTYNQFLDKRVNEFGVYRKLGTESNGEVEFIGEKGTVINNGTII
                     SYRDLLFVVIKDVTIGSEEGDNSPVQALEVGKKYNLPTNCEFKLVDNISGVTKITNTR
                     SFEGGTDIETDEELKERFYKIQRNQATSGNKAHYEEWALEVDGVYNVKVYPRWDGPGT
                     VKVLIFGKNNQAVDTETIERCQQHIDEEKPIGPTITVVTPLPIEISISAVMKLEDGYT
                     LDNVKESFLESINTYFRDIRGEIIYTKVMGILINTTGVHDLSNLLINGSTDNITINED
                     KIPSVTTVNFSEVENQ"
     gene            complement(18051..18479)
                     /locus_tag="MJE33_001502"
     CDS             complement(18051..18479)
                     /locus_tag="MJE33_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBY9647443.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILEKGDF
                     KIIEKKEALKVWIYKCIKTNRYEHEIYSLEYGTELSELIGQKYTKGLTESEASRFIKE
                     ALLINPYILEVNVKSANFNRDILSANVKVSTIYGEVEINV"
     gene            complement(18479..18805)
                     /locus_tag="MJE33_001503"
     CDS             complement(18479..18805)
                     /locus_tag="MJE33_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HBY9647444.1"
                     /translation="MANPINEFIGIIREEGKYHNQPSFFIGKIKSKLPDLKIETNNII
                     LEKEDILIDSWMIDRQLETFDTETNQEHQHEVKNPFIDNFESGDMVIMFRIGEKFAVV
                     SKLVSL"
     gene            complement(18821..20350)
                     /locus_tag="MJE33_001504"
     CDS             complement(18821..20350)
                     /locus_tag="MJE33_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBY9647445.1"
                     /translation="MIINRSKDSSSNEISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFAENRLSVGIIAKTNVKYTKMFIGVNGYDTIMSAYTEASKKTKKKYMIEANLDKFN
                     VIEKGTVTLSVMFEEGFNIINTTFSESMENVKNKVIVVDQYGSKISEKIDNEIFKEVN
                     VIMQKVIQQQENQDVDIDSEFNGIEKSCSLKGYGDVSCITGRGVKVKDSYTKLVGLFY
                     IDTDKHTWQNGEYQIELELNFQNLMDEKSAGQDEPKEESNLGGEDYAGGKEFTAEFTA
                     YCPRKEEGGDTDCRKKKLDPSKKTCAAPMVGKYEQTYYTKEFLNKHPLLNYGDEIQVI
                     TGVSGRDGVYKVNDVGPAITIEKNGTYHIDILFGNVEEASKFGRRKGKIIIGGYSGNV
                     SDKAKIVISEAKKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVSLPRTSNQQSKKGK
                     KVEQKNLQAGDLVFFHNPVSHVGLYIGNGEFLHAPQKGDVVKISKLSSRRDFNTARRV
                     L"
     gene            complement(20632..21054)
                     /locus_tag="MJE33_001505"
     CDS             complement(20632..21054)
                     /locus_tag="MJE33_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647446.1"
                     /translation="MEMWLRQAEDRFRFPVFPSSFSINGKAAVNSSSILKIGEIATFG
                     GVALKSISISSFFPNKDYTFCDYTGFPSPYDCVNKIEKWMKEGFILRFTITETNINME
                     VIIEGFSYEERDGTRDVYFTLDLKEYKRIKIPKVTPKQ"
     gene            complement(21054..23507)
                     /locus_tag="MJE33_001506"
     CDS             complement(21054..23507)
                     /locus_tag="MJE33_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647447.1"
                     /translation="MGNVREEGINMYLTDNYTPKMNQIISVTDNFRRATVAVSLSTNV
                     MASSIKNSIGSASNRVNSLNSSLRKVQTTASSVSSTMTKLSSSINAVSGVIGSLNGSI
                     MRLAITIAMIIDYFNKLIQKKNEFNSNIMIILIFKAKSDEVEKTKNKLLGNLKKIGGK
                     IWNIVIKAKDMTKRVISSILGKLKQVEKRPYQGSINLKDMVSSAMGRILPKLMLFKNT
                     FWSGVIAIKDMASGIISKVFPKLRLFAGKVWSGAIAVKDMASGILGSIKGKISDLTNG
                     ATIGVAVKKGVDLLGQEQNQKVVLESVMKRNTGKVNQIDVDDYYGSLVRMANDTPFDP
                     EDVVAMGTKAKMISNITGGKKEKDITQAMVDVRALNMNTSSEQDVSAAFLSAAKGNME
                     SLNTLVGENYKTFDEALEGISVKQMGLAKEMSNTIPGIISGAQTSINNGLKSIVKSFD
                     DILGQGLKKIKTFIESGLGNLAGLSEKMAGKIGNVMNGKIIIGNKYDQMQSRSVKNGK
                     EFSDSTQYRISNEAEKRKMMVENKQERFENHAATMIGNAPKAIVNAGSTLLQNIDFTA
                     LIDSLLPVVNLVNNLLDSINNKSPIAQGLISIFGTIVTTAFQLIGPVVEAVSPIITRI
                     FTFLGEYAPQINNFIETLGVIWKTVWETLGPLLETGWKIIEPILGAFFNILDKVCKIV
                     KDICKWWQTMINKIKNGSITGTVLNLVEKSKKNYKDNPYAGTKAGDSGKAYSGKKGNN
                     AFGLNYVPYNDYQTRLHEGEMVLTKQEANQYRSRKNGGNINIAKLADTIVIREEADIE
                     KITSKLVASIQLAQLGGVL"
     gene            complement(23507..23674)
                     /locus_tag="MJE33_001507"
     CDS             complement(23507..23674)
                     /locus_tag="MJE33_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647448.1"
                     /translation="MYYYWKKKGIRPSLFYAMDKGELKLIEAFFALEIEEEVEKMKHG
                     YGVCPLTGGGM"
     gene            complement(23701..24147)
                     /locus_tag="MJE33_001508"
     CDS             complement(23701..24147)
                     /locus_tag="MJE33_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBY9647449.1"
                     /translation="MSENGLSKNINIVDLLLNSDTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMGYGKNAVIEEVKN"
     gene            complement(24261..24689)
                     /locus_tag="MJE33_001509"
     CDS             complement(24261..24689)
                     /locus_tag="MJE33_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBY9647450.1"
                     /translation="MANMEARNVMSGTWGELWLDGNKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYMGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            complement(24703..25767)
                     /locus_tag="MJE33_001510"
     CDS             complement(24703..25767)
                     /locus_tag="MJE33_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBY9647451.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGEADIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAVLGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLSDVVDIPKMTKVDAESRVNKGELILIKEAGAIR
                     IARGVNSLTELTAEKGEMFQKIKIVDTLDIIHSDIRKVIIDDYIGKVTNSYDNKCLLI
                     VAIKSYLEELEKSALIESDSTVEIDFEAQKSYLKSKGVDLSYMTLQEIKEANTGSKVF
                     LKAKIKVLDAMEDIDLSIEI"
     gene            complement(25772..26215)
                     /locus_tag="MJE33_001511"
     CDS             complement(25772..26215)
                     /locus_tag="MJE33_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647452.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEGSCFFVSILS
                     VICTPVMLNTNNKDIVISIKYLPKPQSKSIRMYEISDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYVLNFTFTLNYLDSVYEEDVVYENMKEINLNLGE"
     gene            complement(26464..26904)
                     /locus_tag="MJE33_001512"
     CDS             complement(26464..26904)
                     /locus_tag="MJE33_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647453.1"
                     /translation="MENKKDILFKETDKRLHNYKYLDIKIKNINLDIKRCENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKMEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIFKLSEIL"
     gene            complement(27009..27206)
                     /locus_tag="MJE33_001513"
     CDS             complement(27009..27206)
                     /locus_tag="MJE33_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY9647454.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEQNIEEVFFIQL"
     gene            27375..27695
                     /locus_tag="MJE33_001514"
     CDS             27375..27695
                     /locus_tag="MJE33_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBY9647455.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFNVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            27777..28283
                     /locus_tag="MJE33_001515"
     CDS             27777..28283
                     /locus_tag="MJE33_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647456.1"
                     /translation="MNNLDKLFELASQEEIIIHYTTYIAGDLEGLYINKHGIKIISLL
                     SNLKQNSKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIINIINSGITCVYEMADILNVDITFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
     gene            28984..29739
                     /locus_tag="MJE33_001516"
     CDS             28984..29739
                     /locus_tag="MJE33_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBY9647457.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLEDLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
     gene            complement(29882..30310)
                     /locus_tag="MJE33_001517"
     CDS             complement(29882..30310)
                     /locus_tag="MJE33_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBY9647458.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(30731..30931)
                     /locus_tag="MJE33_001518"
     CDS             complement(30731..30931)
                     /locus_tag="MJE33_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBY9647459.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            complement(31114..32283)
                     /locus_tag="MJE33_001519"
     CDS             complement(31114..32283)
                     /locus_tag="MJE33_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HBY9647460.1"
                     /translation="MGGVALKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNM
                     EEKVMESANVEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYE
                     NEVKKMKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQK
                     DKENAFRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSDDIRNYIGQQDGV
                     ELQLTDEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGR
                     FKKNSIWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFV
                     GYEKKGRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
     gene            32519..33340
                     /locus_tag="MJE33_001520"
     CDS             32519..33340
                     /locus_tag="MJE33_001520"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HBY9647461.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVNFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRQEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIYIAKSVSDLGPSKVVITGILEDDNILNLAYNRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
     gene            complement(33416..34804)
                     /locus_tag="MJE33_001521"
     CDS             complement(33416..34804)
                     /locus_tag="MJE33_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase"
                     /protein_id="HBY9647462.1"
                     /translation="MLGGKKSNNKKVEKLRTIFIKYLSLFFIMTISIVLLLMLSFSVL
                     LSSGVILPANYAEKQFNKYKEQIISSEKVTEDIIPSIYEYGVYTLDGNLISGTFNKKE
                     SKEVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKIVSEYSSPILRAYLPKPETLGM
                     IIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNVLF
                     SMDKMKLALKDSLEKQWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEINENPELL
                     EYMEYISKGVTQIEKYINTLIDISKTENGYILNKEAISMSEFIKDVLVQIEALASTKN
                     LDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVHNKYLKFVV
                     TDCGSGFSKADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGTLCIENSVKT
                     GGAMVTIEIPIV"
     gene            complement(34939..35697)
                     /locus_tag="MJE33_001522"
     CDS             complement(34939..35697)
                     /locus_tag="MJE33_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBY9647463.1"
                     /translation="MRQLIRIFCSDLIKLKRNFIILMHICIALMGMGLFLWYYKVSGA
                     DNILKIVAYLQVIAIAFPLLSSVVCSMFVEQEYYAGNYKHMLTTSNPKYLTLISKYII
                     LVCLGFVATLVSVLGFKFGISKAYFTSSFYMISILILIGCNLFEYILHLFLSLRFGKG
                     TSIGVGIVEMLLSALLLTGLGSGIWQYFPCAWGVRFITIWSSFSSSKTVEYIKVESIK
                     AYQSTGLICGFVTILAFVILCIWFSKWEGKKSEE"
     gene            complement(35699..36415)
                     /locus_tag="MJE33_001523"
     CDS             complement(35699..36415)
                     /locus_tag="MJE33_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBY9647464.1"
                     /translation="MERFIRVEFQKMKGTFGMKLIWIVPVFCILLCGLGGGQNAAFNW
                     WYTTFLPGALTLISSLIVLKDEKSKYRGILPLPIEMKDVWTGKMIYCIICCFIASIIS
                     AIGVVFVGIIYKETISIGQNIIASILIILLFMWQIPLCMFLASKFGMFVTVLTNMFFT
                     IVGVAVFSTTEMWYMFPYSIPSRLMAVVLHILPNGLPVPEGSEFLNNAVILPGVLISI
                     VLFVLFALATRVWFNNREVK"
     gene            complement(36417..37124)
                     /locus_tag="MJE33_001524"
     CDS             complement(36417..37124)
                     /locus_tag="MJE33_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBY9647465.1"
                     /translation="MSNYILQVKGVSKKFKEQMAVDNISLAIKSNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWTRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLETVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRELIRSFPKQGITVILSSHILSEVEQVVDEIGIISNGVMGYQGEVSKEQN
                     LEELFMKVVAENRKRGQ"
     gene            complement(37240..37776)
                     /locus_tag="MJE33_001525"
     CDS             complement(37240..37776)
                     /locus_tag="MJE33_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBY9647466.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDED
                     AKEETTSKMINSKKTTKIPIEIISKDEKIVKYLQIDEESSLKDKLRLILDTLSNEYFN
                     GLDMEVEVKEKDNLVKINLIEPDKKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            complement(37878..38948)
                     /locus_tag="MJE33_001526"
     CDS             complement(37878..38948)
                     /locus_tag="MJE33_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBY9647467.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAELNK
                     ENKLYQEKVNIDKLDIAVDNINVMIRKSSNEDVRVTTRGNNEFYKYSVTLQDKTLVVK
                     GERKSENKIKKIRSFDHLLNTSINSMFSRDYREIRIYVPNNVDINASSISSYLFISDD
                     VDSNNITYKTSSGGFSRAITENKVNRLENLNLISNSNLHLSTKSILGVKNVNIESESL
                     DISSENGDVFINNIEEYIPENVNIKEKIGRNSNYESEFYLYSDMPIAKFLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEAIEEFNNDEEYDNDSYDYEHNEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNSLEL"
     gene            complement(38941..39999)
                     /locus_tag="MJE33_001527"
     CDS             complement(38941..39999)
                     /locus_tag="MJE33_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBY9647468.1"
                     /translation="MNLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIIASLGSPKSIAKELILQTKEKNIEEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     DEAKEKYSKSKENIDGKIMNTDILNGKFSRFKSNVKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSATIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGLEILAWQILLFAIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLNDEDLNKDINENSYNNDYYNREEESIKETDEYNDDSINFEA
                     IYKDLEDIENKKGEDKHE"
     gene            complement(39986..40318)
                     /locus_tag="MJE33_001528"
     CDS             complement(39986..40318)
                     /locus_tag="MJE33_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBY9647469.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DEGGNELE"
     gene            40517..41638
                     /locus_tag="MJE33_001529"
     CDS             40517..41638
                     /locus_tag="MJE33_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HBY9647470.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKECIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDLSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKELCCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSNSIEITIVTGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYKLVS
                     NEFDSIINFMKKNLKEFKTVIHPEKIADYISELIDNHYS"
     gene            complement(41766..43664)
                     /locus_tag="MJE33_001530"
     CDS             complement(41766..43664)
                     /locus_tag="MJE33_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HBY9647471.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLEDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVNPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEAGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAIENKDYQTASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
BASE COUNT        14507 a         7245 c         4861 g        17211 t
ORIGIN      
        1 accatttaag gaatattttt gcaattattt taattgaaca ttcatttgta tttctttttc
       61 aaaaaatctt tttaccattt tttagttaat aattaattat tttcacttaa aataagggct
      121 ttaaccttca tttttaaagt atttttcatt tttaatcact ttatatgcaa gacagaatca
      181 tttttcttca ttcatttgtt tctttcatct atataattat cattctttgt aagtaaaata
      241 tctttatatt tttataatta attctttata tagttttaag tataataaaa tcatgtatac
      301 taaatgcatg ttaaaatatt attttaaaac atgcacaata tttgtacata ttgtagacat
      361 ataaacctat aaattataca atagtattgt taagattata tattttttta acataaattt
      421 tgtattgggg gaaaaacaat ggataaaaac tatacgatgc caatatacca aaaaatagcg
      481 ttggatattg caaataaaat atacacgggt gaaatacaag aggattcagt actatttgga
      541 cgctctgttt tggctggtaa atacaatgta tctccagaaa cgataagaag agctgtcaaa
      601 attttagagg acattggcgt tgttaaaagc ataaagggta aaggtgttat tgtactatct
      661 ccagacaaag cttcttcttt tataaaaaaa tatagagata ttacaaatat atcatcatat
      721 aaatcaacac tttataattt aatagatact aaatctaatt tagaaaatga aatattagat
      781 actataaata aaattttaga ctattctaac agacttgaaa taatcaatcc tttagtgccc
      841 gttcagttca ccataaattc taattgcaaa tatattggtc aaacagctgc acaaactaaa
      901 ttttggcaaa atacaggtgc tacaatagtt gcaattaaaa gaggtgaaga acttataata
      961 tctcctggac catatattga atttttagaa ggagacattc tcttagtagt tggtgaccaa
     1021 cacatttaca attctatacc aatgttttta tatgaaaacg aaaaataaat tatataattt
     1081 agtgtgatta tataaaaatg caagattaac aacaaaaaaa gagcttcata ttttacataa
     1141 agctcttttt aaattcaaat aaatttaact ttgattaatt tttaaatatc caattcatct
     1201 tctttttccc agcttaaagc tctcgataca gcttttttcc atcctttata taatttacat
     1261 ctcttctctt cactcatatt tggattaaac tctttttcta ctgaccactt tgaagtaatt
     1321 tcatttcttc caatatagaa tccaacagac aaccctgcta aataagcagc tcctaaagct
     1381 gtagtttcta ctattttggg tctatctata tctacattta atatatctga ttgaaattgc
     1441 attaaaaaat tattattact tgctccacca tcaactttta atgattttag ttttaatttt
     1501 gaatcgtttt gcattgcttc taaaacatcc tttgtctgat aagcaattga ttccaatgtg
     1561 gctcttacta tatgttcttt ttttgctcct cttgtaagtc ctaaaattga acccctagca
     1621 tacatatccc aatatggagc tccaagacct gtaaatgctg gcactacata tacaccatta
     1681 gtatcctcta ccctatttgc atacatttca ctatcttttg cagattcaat tatctttaat
     1741 tcatctctaa gccattgaac agatgctcct ccaataaata tacttccttc taatgcatat
     1801 gttacttttc catcaactcc ccaagcaatt gttgtaagta acccatgctt agatttaact
     1861 atgttactgc ctgtattcat aagcaagaaa caacccgttc cataagtatt ttttgcagta
     1921 ccttcttcaa cacaattttg accaaataat gctgcttgtt ggtctccagc acatcccgca
     1981 ataggtattt tagctcctga taacatgcgc tcatctgtat gaccataaac atagctagaa
     2041 ggttttacta atggcaacat gcttattggt atgtccaatt ctcgtaatat atcctcatcc
     2101 cattctaaat catttatatt gtacatcatt gttctagatg catttgagta atctgttaca
     2161 tgtacttttc cacgagttaa attccatatt aaccatgtat ctatagttcc aaataataac
     2221 tctccttgtt ctgctttttc cctagctcct tctacattat ctaatatcca ttttactttt
     2281 gttgctgaaa agtatgcatc tattaaaaga cctgtttttt cttttattga ttcttccaat
     2341 cctttatttt taagttcatc acatatttct gacgttctcc tacactgcca tactattgcg
     2401 ttataaatag gtttaccagt atacctactc caaacaaccg tagtctctct ttgattagtt
     2461 atccctattg aagcaatttc ttctggtctt atacctgctg tttctataac ttctctcatt
     2521 accccacttt gtgaccccca tatttccata gggtcatgct ctacccatcc agcttttgga
     2581 taaatttgat tgaactcttt ttgtgcaatt ttcactattt cgcctttttt attaaatagt
     2641 atagctcttg aactggttgt tccttggtct aatgccatta catacttctt ttccatcacc
     2701 atattctcct tatcatataa tatttaattt attaaaaaga aaaatttaat atcacatatt
     2761 tcaataaata atcatactta agtacaaatt taatataata gatatttagt aagaagacac
     2821 ttattttcta tcatatctat ataataatat tatacattat agcttacata ttgattaata
     2881 aattttaaag gagtataata aacagtgttc ataataattt taataaaaga ggtggtaata
     2941 tgccaaagat tttagaaaat gtaaaagaag atatattaaa agtgtctcgg gatatgattt
     3001 tagaagaaga ttactcgaat attagcataa ggaaaatagc tcaaaggtgt ggtataagtg
     3061 caggtacagt gtataattac tttaattcaa aacaagaaat aatagaatat atcacaaaat
     3121 ctgaatggga tttattgata aggcgaattg aatactccaa taaaaacact gaagattaca
     3181 taaaaaaact aaacattatt tttactgaaa taagaaattt tataaacaaa gttcataata
     3241 tccaatacaa tgacttttta aacacttttg aaacagagag attttttgaa atgaaaaaac
     3301 acaaagacga ctttcatgaa cagctttcaa ataaagtata tgaagcttta gaaaaagaaa
     3361 gtttttttaa taatgacaaa ttagtatgca atatcataat aaaaatgttt ttttcatatt
     3421 caacatatct tcatatagaa tttgaagatt taaaacccta tattgaaaaa ttaatacatt
     3481 aaggtggtgt ttaccttgaa tcataatact agtattaaga acaaggacag taatccaaaa
     3541 atatttttag tacttacgct ttatctatta ggtatattta tgggagctat tgatactgga
     3601 atagtatcac cagcaagaac gattatacaa aatagccttg gagtaaatga aaaaacggga
     3661 atttggatga ttaccatata cacattaaca tacgcttctg taattcctat ctctggaaaa
     3721 ttagcagata aaataggaag aaaatatgtg tatctagtta gtatatttct atttggtagt
     3781 ggttcattaa tatgtggatt atcatcacta ttttccaatt tctatatttt attaattggt
     3841 cgtgttattc aagcaattgg tggtggtgga atcatgccaa ttgctacagc agaatttgga
     3901 actaccttcc cagaaaataa aagaggcatg gctttaggtt tagttggggc aacatatggt
     3961 attgccaata ttttaggttc aagcattggt agcacaattc taagtatctt tggaactcaa
     4021 aattggaagt ggctattttt tgtaaatctt ccaatttgtt taatcatact tattggtgga
     4081 gttttctgta ttaaaaacaa taagtctgaa tctaaagaaa aaatagataa gcttggtaca
     4141 ttgatgttga tatgtataat cgtatcacta ttatatggtc ttatgaatat agactttttt
     4201 aattttaaaa attctataca agatatatca gtttatccat acttattgac atttattgta
     4261 cttataccta ttttcatatt tattgaaaat agagctaaag accctatttt aagctttgaa
     4321 tattttttaa atccgagaat attgatagta cttattttat ctttaattgt tggtattgga
     4381 atgatgggta tggtattcgt tccacagtat gctgaaaatg cattaaagat aaatgctggt
     4441 tctgggggat attttgtagc tatattggga ttatttgcag gtattgctgc tcctttaagc
     4501 ggaagactta ttgacaagtt tggggcaaaa aaaatactat tactaggatt ttctgtatct
     4561 atgataggct ctctttacct agttttaatt gctctaaaaa ccaatactgt atttagtgta
     4621 tgtgtaagtt taatatttat gggattagga atgggattta ctatgggaac tcctctaaac
     4681 tatatgatgt tatcaaatac aagattagaa gaatctaatt cagcacttgc aacattatcc
     4741 ttgatacgct caattggtac ttcaatttct ccagctataa tgattggatt tatagcacat
     4801 gcaggattat ctgtgcaaga taatattatg gacttagttg gaaagccctc aactcctaaa
     4861 atagttcaac ttgaagaact tgaaaacatg atagatgact taaaatcaga ccctgaaatg
     4921 gcaaaaaatt taaaaaatgt ctctatccca aatatgaatg atacttcaaa tataaatatg
     4981 gacatgacag gtggcaaact tccaaaatcc ttacttaata aagttcagtc agcagataca
     5041 accaacatta ccaatataac aaaagaaata tcaacaacaa tgtttgatga aaaagttcca
     5101 tctataatta ctaaaataca gaaaaatgta caaaaaggaa tagatggaac acaaagtgga
     5161 ataaacggta ttgaagatgg tgaaaataaa ctaaatagtg gtattaaagg agttcaaact
     5221 ggtattgaca atatggtaaa agctagagct ggtattaatc aaggaataga tggtgtaaaa
     5281 aaaggtattg ctggtgttga taaaggtata aaaggtatgg aacaaggtct tgaaaaacaa
     5341 gatgaggcca tcaaggaact tacaactgct tataataaaa tacctactac agaaaaaact
     5401 cctactgata acgaaaataa ctcaaatgaa caaaaccaga gtaataccag taattctgaa
     5461 tcagacgaca ctaagaatga ttctacagaa aataagaata acaattccag cgagaataaa
     5521 ccaaatgtaa atgaacaaaa agaaagttta aatgctcaaa tacagcaact aaaaaagaca
     5581 agagatgact taaatacaaa gttacaaaaa aataaaactc aaaagaacga attaagtaaa
     5641 aaactaagat caatggaatc tcaaaagaaa gagctacaaa gcaagttaaa taattctata
     5701 agccaaaaga aagacatgga aaaaagcctt gatacaatgc aacagcaaaa aaaatcactc
     5761 caagcagttt tagcaaagac acaagaggta aaaactgaaa taccaaaggc ttttgataaa
     5821 tcaaaattgg attatataaa ttccatagaa aataatagaa caaaaataga aaacacacta
     5881 caatctactc taaactcagg atttaaacag atgtatataa cagtattctg tgtaaatcta
     5941 ctagcattta taatattatt gttctataaa gaaaataaaa ctagataaaa gagtgttata
     6001 gtattatatt gtaaatttat cttattctta aatattttat tctaaagaat aaattcataa
     6061 aatttttgta attgattaat ataaattaca atataatact atttttctct taatcttttt
     6121 ctcttttaca gacaatcaaa aagcaatctt agtttatcga aataaattta tacaatctat
     6181 tcatacaccc ctttacattt atataacaat taaacctatt gttatatata caaatcaaaa
     6241 taaaccatat attttcaact ataatatgtt aaaatcatat tatgagcata ttaatttaag
     6301 caaaatgtca tttagacttt aatttagtag ttttatatta taaatttata tatttttaat
     6361 tttaaatgtc atttagacat tactcataaa aagaaaggag tgtattttgt ggatacttta
     6421 ggaaaaagaa ttgcttattt gagaaactcc aaaaaactta ctcagcgcaa acttatggat
     6481 attttaaaat ttgaaaactt aggcaagtat gaaacaggag atagaaaacc taattgtgat
     6541 atactaatgt caatagctga ttattttaat gttacgactg actggcttct ctatggtaaa
     6601 gagaaggtaa atgtcaatag tagtgtaaaa gaagataaag aagattactt acatgttact
     6661 aatgatgaaa tgacgatatt aaatctatac agacaactaa acgaacgtga taaaataaaa
     6721 atagaaggaa tacttgaact taagatatct gaatataaag acttaaaaaa gcattcctct
     6781 aataataatg aagataaaat ggtttaaaca tacataattt gatacgattt tacatcatct
     6841 attaaccttc tttttttgta gtatattact aaaatatgga tattggatta tttaaaaaca
     6901 agtatagtct aatttcaagc acatttataa tatttagatt atcttatcca tattttagtt
     6961 taagtacttt gaaaaatata atacatctct atgtataact tatattccta agaaatgcat
     7021 tactcatgtt tttaattctt ccaacatctt tctatacaca attgtacatc cttcataatt
     7081 cccctcttta agaaggattg aacatgtact agaatattta gttaaagtat ttaatctttc
     7141 catatttaag ctttttatat tactgagtgc tgtaataaaa tcatcaattt ctctacaaca
     7201 ctctataacc ccatctgaat ccaatttaca cacaagaata ttcacctact ttatatacta
     7261 tttattaatc atcttctgaa tattctatat taagtaattg ataaattgat gatttatatt
     7321 ttttacctgt tcttcttcca tttaaaatag ctgacaaata gttttcatta acacctattt
     7381 tcttcgctaa ttccctttga gtcatattta attcaataag tctttgcttg acttgcactc
     7441 ccaaaaaagg tatccatttt tttcgagcat ccatttatca acttcctttc taagtgattt
     7501 atatttactt ttatattatt ttagcttaca caccccctct atttagtata tatttttaat
     7561 atgtacacat attaagtata taaatttatt taatactaaa taagattttt tgattacttt
     7621 gtgctaaaat taggatgtaa attcgtatca ttaagttaat tataaagtca ttttgacctt
     7681 aagtcaatgc tttttatgtc attttgacct taaatttgaa actatttaca tcttaaaaag
     7741 tctttttgac attaattgaa tggagagtgt ttatggatac tttaggagaa agaattgttt
     7801 atttgagaaa agcaaaaaac cttaaacaat atgaactaga agaaatgttg ggctgtgaca
     7861 acttaagtaa atttgagaga aacattagaa aaccaaatta tgaaatatta aaatctatag
     7921 ctgagatatt taatgtttca gtagactggc tattaaatgg agataatcta tcacataaat
     7981 cagatttaat ttgcgattcc tcctcaaact atccacttaa tagcataaac tctaatgaaa
     8041 taaagctttt aaacaatttt agaaagttaa atgattatga taaagccaaa atagaaggta
     8101 tgattgaatt aaaacttcat gaatatgaaa aagagaagga tttaggaaaa attgagtata
     8161 acaaaaataa agatgagaaa atagataaat aatttttatg ttgcttttga tacgatttta
     8221 catcatttct ttgttctaat atttttattt catttatttt cttttttata attttatgat
     8281 aaaataatta gtatattttg ctttacttga atgtaattat ttatacaaaa gtcaaacata
     8341 atataaatgt agtaaatttt tagtttcaag taatatctcg ttattataac taaatattta
     8401 ttaaaattag gaggataaaa tgggcttaag agacaaattc gcgcaatcat ttgctagatc
     8461 aaaaactatg agtggccctg agaaaaaggc taatgaaata atgggtaaat tgcttcttaa
     8521 gaaagctata ttacctatcg ttttaatgtt cgtgataata atcgctggag ctatgcttaa
     8581 aatcaattct tgggttacac ttggcataaa tttagttatt gctgttggtg ctttcttcta
     8641 cataagaaat tctagtaaaa agtatcagaa ctttaaacct tatgttggca atttgattag
     8701 tttagagaaa aaaggtaaaa aagagtacgt tgctattata aaacaaggta agttaccagt
     8761 taagttacaa atagcttatg gtggtgaaga ccttgaacat gttaagaaaa atcaaatggt
     8821 tcaaataagt tataatccag atgctaaaat agctattttg gttaatagac aataatttaa
     8881 taaaaaaaag gagttgtctt aaaattgatt ttttctaatc aaatttaagc tcaactcctt
     8941 ttttaatttt tatatattat ttttatatat tatttttatt acttttttta attaatattt
     9001 ttaaatatca aatccttctc aataaaatag ggtactatct acaatactac tttatctttt
     9061 cctaacaata tataatctgg tgctactctt tcatgttata caccgttttg taacatacct
     9121 tttataccag taacatcttc cttaatttcc tctacatctg ttttcattat acccatttga
     9181 acaagtatat ttttgtttat ttcttgttgt tgtgtagata attctataaa attttctaca
     9241 gtttttttat acatgtctct atcttctttt ttctcctgca tagtattttt aaataataga
     9301 gcacatagta tacctattgc ccctagacta ctcaattcgg ttattaattt atccattata
     9361 gcctcctatt gtctaaattt tttatactta tagctttaat ttttgttata ttcttacatc
     9421 atgcaatatt atattttcag gattatcaat ttaatcttct gtactttaaa ttgtaggtgc
     9481 aatttataga ttgaataaat aataaatata tatacaattt ctaatataac aaaaaataag
     9541 tacaaggctt acttattaaa gttaagacct tgtactttac ataaaaaaaa taactaatac
     9601 atagttattc taaatttact attcaactgg tatttctaat tctttgcatc taattctaac
     9661 ttgctctcta attactgctg gtacttcttc tattgttttt ttgcctctta aaattaaagc
     9721 tacatatact tctaccattc cactctcacc tccagatttt cttacactaa aagagcttat
     9781 attacttgct tcattgacag aactttgatt taacaacatc tcatatattt ctgcatttgc
     9841 tataaaatta tttgttatat catcatcttg ctttacttga cctatactta tgtctgaaat
     9901 gtctttatac tgcatttcta cttttctacc ttgtttgatt tgttctattt tatttttatc
     9961 gaatttaaac aattttcaca cctccatttt aagcttgtgg ctcgttgctt tcagcatcat
    10021 atctaaaaat catagatgga cgttttatag ctgtccattt atctcctttt tctactatga
    10081 ttttaggctg agcacttttt atgttggctt cttctaattc atagatttct tggtaacttg
    10141 agtctgattt tttgatttca actagtttca tgtctttgaa taagtcagat ttatttattt
    10201 ttacttgaac tttagtttta tttattaatt gatatatagg attgttttcc atcatgtcat
    10261 cctttaatcc ctcatttaat gttactatta aatcagtgtt attttcagta atgtttatga
    10321 ttgaatatac tttattgttt atcattaaaa tagaatcctt ttttacttta agccctgttt
    10381 ctgatttaaa tataaacata cttgaatata aatcgcctgt aggactactg gctttaatca
    10441 gtatcttatt ttctcctaat actattttat tttgtggtat ctcaaatata atgctattta
    10501 tttccaaatt agtcttagta gatattaatt catcatttat aaacatttca agtttttcta
    10561 taacattatt agggtcttgt actacaactc tcacttgaga tattgatttt tcaactattt
    10621 ctatagatgc tttacttgta tctacttttg ttatcatgta tattgtatca tctgaaagct
    10681 ctatatctaa aatatcattt agatgttcag gaattggtat tttagtaaat tcatataaat
    10741 cagtaattga tatagatatt ttatacatag atttattttt accagttatc catagactat
    10801 tatcattttt aattactagt atgtggtcat cattatgtga cttaatttct ttaacattag
    10861 ttaatacttt agtaaaagtt cttattatct tatctttttc actttctcct agtccaaata
    10921 atccatttgt attgtaccct tgagcgtaca actctccctc attagttaaa acaagtgtca
    10981 ttgagttata tatacttata tctttaacag aaggtaatcc agtaaccttt tgtaactctc
    11041 ttacatcatt attgtggcca actcccaatt ggtactcagt attctttcca tatgaccata
    11101 gagtattatc tgtcttaata atataggagt agttatctcc acaagcaact tttttgacat
    11161 ctgatatatt tacttcagta agagttaata attctatatc ttcaagggtt tgattaattt
    11221 ctttatatga attacttcct attccatata acttgccatc cttagcaaca gccaatgaat
    11281 gtgtttttga tatatcaata tcagatacct caaatggcaa tattattgaa gtatacttaa
    11341 aattatcttt aaatatgttt ttataattta tatacttatt atttgttttt atatacttat
    11401 cattaaacat tactagcttt tcatttttct tattaaaaac tattttatct gatgtgttat
    11461 ctgttttaaa atatgaatgt agggaacctt caagagcttc atttttatca ttatcatatc
    11521 tatggtgaat acctaaatga ttatatctat tatctcctgt tgaagaataa aattttccat
    11581 atatatcttt aataaatata gtattgtaac tagaaaatac agtatcaaca tctttcttta
    11641 tagatacttt ataatatgga actgacatat ccctataaga ttcatcagat ataccataag
    11701 tatctctacc tgatgatatc aaattgttat tattatctat ataaagtgta ttattagctg
    11761 ttacaaaaac atctttaaca tcttccacac tatttgtatt attattattt gctatacttc
    11821 catatccact aagacctcca acaaatagtt tattgtcatt tgttaaaatg gctgaagtat
    11881 atccattaac tactattttt tttgcattta taatattatt aattactcgt atacccttta
    11941 tatcattagt ttctgttgaa aagaataccc tctctttttt ccctgtagta taaataacat
    12001 tgtcatttgt tataaaaaat gtagcttcac ttcctgctgt tacaacagat tttacattct
    12061 caatatttat ttttgtaaat gtacttatac ttccttcagt aaacccaaaa tatccattta
    12121 caaatccaca tacataacat gtattatcat ttttaacaat tattgataca tcattacata
    12181 tatacacatc tctaactcca tttataggta tacgtgtaaa tatattccta cttgtagtat
    12241 cacccaatcc taactgtcct tttccattat tacctgctcc atataaaaga ccatcattag
    12301 ttaataatag agatgtgttt ccatatacag ctacatattt tacattttca acatttattt
    12361 tagtaaatac atttctattt acattatctc ctattcctaa ctgaccagaa ttatttaaac
    12421 caacagcata acaggtatta tcatttttta tcgcaaatac atgtttacta ttgtcagtaa
    12481 atctatttac atttatagat tttatattat caatatttat tttagtaaat acctttctat
    12541 ttatctcatc tcccactcct aattgaccta catcatttaa accaacactg taaagcatat
    12601 tattattagt taataaatat gtagacttcg aatggctata tatttcttgt atattagaag
    12661 atatttcact actgctattc tttttattgt aatttatctt attatttaaa tttaaactac
    12721 tattaactga tataccatgc gtacccttag agttatatcc acatataaat aagctcttat
    12781 cagttaatat tgcaaatcta gttttatctc tacttaaatc ataaggaggt ataaatatat
    12841 tttttatatt tttcattacc tttttataat tagtggcatt atatccatct tgtgtatctg
    12901 aaacatctag ttcttttgca ctttcagata tattcccaaa accatataac gtgttagaag
    12961 ataaatacaa tagagcttta tataaaaata tggctttatc agaggtaata ggacctgaat
    13021 ttatattaga tgctgcaata ttattagatg attgattgtt aagataattc cttccatatt
    13081 gatgtatata agatgaacct ccagaaataa gtaaattatc tggatttaca ttttttaaat
    13141 tacttgttga ttctccataa tacaaatagg caaaatttgt agaatatgat gattgtgtac
    13201 accttgtggg ataatagtta tatatgtaat tactaatata tccagcatgt gtaattcctt
    13261 gttgttgaac tctataagta gaatcacttg gctttgtcca taaagtacca tggtatccag
    13321 ttaaaaaaga accataattt gtacataata tagccatata ttcatatata ataatattat
    13381 taattttagt aatatcattt gtacaagaat ttgtactata acttgctcct gaaggattta
    13441 cataaaacca gttatttatt aatgttaaat ttatattgac tccatcatta ggattaacac
    13501 ccaataaata gtttgtattt ataccagttg catatacctt tttttcgtca gccacaataa
    13561 atgttgcatt atatgaacta tatgcatctg ttacattgct aacaccaatc tcagtatatt
    13621 gacttgctga tttattacta agtccattaa cataacttga actctttcca gaagtatata
    13681 tttgcttatt attagttaca attaatgaaa accagtttga taaatcagta tttataacaa
    13741 ctttttttac attatctata gatattttct taaatatatc tgaattagaa ggaagaacac
    13801 ctgctattga gttactattt ggcccagaat aataacaagt gtcatcattt ttaatgtaga
    13861 atattcctcc agaaatattt tttgatgcta ctatagactt tacattagag atacttaatt
    13921 ttgtcaactc atttaattga cttggtaagt cagaacccat tcctttgtat ctattatttc
    13981 ctagaccata tacatctcca ttccctaaaa taaccagagt acaataagga ctaaaaacga
    14041 catctcttac agaatgtata tctagtggaa gttcaatatc ctttatttcc atttcttctt
    14101 ttaataaagt taatttatcc tcagatttaa ttaaatttat ataattacta taactatatt
    14161 tttcactatc cattttctta aattctacaa tgtgcatatc atcaaaaaac gaacctaatt
    14221 gtccataagt attatctcct gtacaatatg aatcattatt tgaatcaatt agtatgctat
    14281 gtgataaacc aatgaaaata ttcttagcta caatattttt taaatctata aatgaattat
    14341 ctatattctc agagtaattt gctataccta actgatatat atcattattt ccagcaacct
    14401 ttaaagtacc tttgttatct ataagcatag tgttaccatg accacatgca atagttttta
    14461 tatttacttc ttctatctta gtaaatagtg atgattgaag tgcatttcca aaacattttt
    14521 cctttccaca accatatata tcattattat acgtcattaa cacagtgtgt tcagaaccac
    14581 aagctatagt cttaacatca ttaatagaaa ctaaactaaa taaatctcta ttattcttat
    14641 ctcctaaacc tagttgacct tgattattaa aaccagtaga atagcattta ttgtcattct
    14701 ttattataaa agctgattta ttatatgaaa atacagcctt tacattgtca acatctactt
    14761 ttgtaaatgt atttacattt gcagatgctc cattagttcc tagacctaat tgaccataca
    14821 aattatttcc agcaacatat aaagttccat cattgtataa aagatatgtt gattgaagtc
    14881 cacatgcaat atcctttacg ccttttattt ctggaacctt ctcaaatgta gaaactgttt
    14941 tccctacttt ttctaatccc aatcccattt gaccatctgt atttatacct gcgacaagta
    15001 attctccatc tgaagtaagt ataactgcat gagattctcc acatgcaact tttttaatat
    15061 tggtagatat atttggtatt ttcgtaaata attttcttgt tgtattgtct tctcccaatc
    15121 caagttgaaa gttactatta tctccacatc catacaagat attattattt tttagaccaa
    15181 atgtatagtt acaacatcca gtaaaaaaat taataaagtc ataattatca tacccttcat
    15241 caaattcatc aactaccata tttttatcgc caaagaaatt acctctttga agtagtttag
    15301 ttcttttcat tataaaaacc tcctaattat cttatttcat acgaatattt atttaaagtt
    15361 atatctgtct gaccaattga atatgcccta ttagattttg tttcaacaaa gttattatcc
    15421 atttcatgta tatatctaac tctatttgca ttaaatggta gcaccacatt cttaaatgta
    15481 acactaccaa gttctccagt tatacacttt ctatttaaat ctaacttaag gccttctata
    15541 gctattatgt ttttagcatt agttaaggta tcataccaat atccagcttc atctacatct
    15601 atttctgact ctaattctaa gtatattaag atatctaata ctttttcaga taatttattg
    15661 taactatttt ttaatacaga tagttcagtt tgtatttgac ctcctattac atcatcaata
    15721 gatatatttt taattttttc tactacctca tttatagctc ctgataagtt tttagcttct
    15781 gtagataaga gttcttcgcc aatttttact tttatttctt ttatattttt atcaaactta
    15841 tcatctaaat cttgaatatc cttttgagtt acaaaaacca acgtcgggtc tacttctaat
    15901 gtaacatttg aagtattgga caattgtaaa atcattttta caaccaaatc tttaatactg
    15961 ccatcttcag cacgtggctt atacgtctct ggatacttac ctatagctat tatattattc
    16021 tcattatcca gaacagcagc ttctcttatc ataaatccac caactgttcc tggtaaaata
    16081 gtctctataa ctatccaatt aggatttttt tcatcaattc ttagagaatt tatatttcct
    16141 tcccaaacta cattctttaa agcagtttgt tcctctgttg gctcataata ttctccatta
    16201 ctatctccaa cttgaaactt ggttaatcca acaagctctc ctagtgcagt tgcatttgct
    16261 attttttctc taccaatttt ggttaatatt atataaaatt tttcttccaa ttataccacc
    16321 tccgatttag gataaactac tacattttct acacctgtat catttgcaat agctatatta
    16381 atcttacaat tattttcaat gcatttaatg cttaaaggat aaacctttat ttcttctcta
    16441 cttatcattc cagctccaca gtttacaaca gcactacatt ttataattgg ttcaaagtat
    16501 ggatgaactt ttacctcttc actacatacc atacctccac cacaatagac catactttta
    16561 tctagtatta ttggttctaa atagtgtaat aagtgagcag gttttactct ttcaatcata
    16621 tcgctacaat ctaaaagagt tttatagtca cagttatttg ctataaaact caatacgaat
    16681 gtaaattcat cactataaac ttttatatct gtttctgatt ttgtatatgc ctcacatata
    16741 ctttttataa cttcaatagt actagtacct ctgcttctca ttttagcttt tatattgcta
    16801 cgtcttgtgt caaaatcaag ttcttttttt tcaatgcaga gtatgtcttc ccacatatct
    16861 aatccccatg ttgcagtgtc aacaaataat tggttaaagg tatcatcaat atcttcttta
    16921 agaatattta attctgtatc gtatgcacct tgtatctcct ctacaatgta atttctatca
    16981 aatgatggta gtttatcaat tagcttcatt gattttccac ctcactaaaa ttaacagttg
    17041 ttacactagg tattttatct tcattaatag ttatattatc tgtacttcca tttataagta
    17101 ggttacttaa atcgtgtaca ccagtagtat ttataagtat tcccattact tttgtataga
    17161 ttatctctcc tctaatatct ctaaagtatg tatttatact ttctaggaaa gattctttta
    17221 cattgtctaa tgtatatcca tcttctagtt tcattactgc acttatactt atttctattg
    17281 gtaatggtgt cacaactgtt atagttggtc caataggctt ctcttcatct atatgttgct
    17341 gacacctttc aattgtttct gtatcaacag cttgattatt tttcccaaat atcaagacct
    17401 taactgttcc cggaccatcc catcttggat aaaccttaac attatagact ccatctactt
    17461 ccaaagccca ttcttcatag tgagctttat ttccacttgt agcttgattt ctttggattt
    17521 tataaaatct ttcttttagt tcttcatctg tctctatatc tgtaccacct tcaaaacttc
    17581 ttgtgttagt aatctttgtt actccagata tattatcaac tagtttaaat tcacaatttg
    17641 taggtaaatt atatttctta ccaacttcca gagcttgaac tgggctattg tcaccttctt
    17701 cactaccaat agttacatct tttattacta caaatagtaa atctctatat gatattattg
    17761 tgccattatt tattacagta cctttctctc caataaattc aacttctcca tttgactctg
    17821 tacctaactt tctatataca ccaaattcat tgactctttt atcaagaaac tggttatatg
    17881 tgtcttgtat aaaagccatt ttatgcatct ttgaaagttc tagatatatc ttcgaaagtt
    17941 ctagattatt tccagatacc atgttgttta gaaaagaacc ttctccttta taaatatcaa
    18001 gattaatatt ttcaagagtt ctatttttta ttacttcata tgtctggtca ctatacattt
    18061 atttccacct ccccatagat agtggatact tttacatttg cactcaatat gtctctgtta
    18121 aagttagcac tttttacgtt tacttctaat atatatggat ttattagaag ggcctctttt
    18181 atgaatctac tagcttcact ttctgtaaga ccttttgtat atttttgtcc tattagttct
    18241 gaaagctctg tcccatattc taaagagtat atctcatgtt catatctatt tgtctttata
    18301 cacttgtata tccaaacttt taaggcttct tttttttcaa ttattttaaa gtcacctttt
    18361 tctaaaatag gttcatcttt ttcaaaatcc catgccactt cacgaaagat tggcaattct
    18421 tctgttttag gtaagatata atcctctggg acacctataa aaggaaatat tgtactcatt
    18481 ataagctcac caacttactt acaacagcaa atttttcgcc tattctaaac attattacca
    18541 tatccccaga ttcaaagtta tctataaaag gattttttac ttcatgctgg tgttcttgat
    18601 ttgtttctgt gtcaaatgtt tctagctgtc tatcaatcat ccaactatct atcaaaatat
    18661 cttctttttc taatatgatg ttatttgtct ctatttttaa atctggtaat ttacttttaa
    18721 tttttccaat aaaaaaagaa ggttgattat gatactttcc ttcttctctt attattccta
    18781 taaattcatt tattggatta gccattatat caccatcctt ttataatact ctcctagctg
    18841 tgttaaaatc tcttctacta cttaacttac ttattttaac tacatcacct ttttgtggag
    18901 catgtaaaaa ttctccatta cctatatata atccaacatg gctgactgga ttatgaaaaa
    18961 atactaaatc tcctgcttga agattttttt gttctacttt cttgcctttt ttagattgtt
    19021 gatttgacgt tcttggcaaa ctaacattaa cttttttaaa acaatagacc attaaaccag
    19081 aacagtcaaa actacttggt ccatttccac cccatttata aggtttacct agatgttttt
    19141 ttgcttctga tattactatt ttagctttat cagatacatt accagaataa ccaccaataa
    19201 taatttttcc ttttcttctt ccaaatttac tagcttcttc aacatttcca aataaaatat
    19261 ctatatggta tgttccattc ttttctatag ttattgcagg tcctacgtca tttactttat
    19321 agactccatc acgaccagaa actcctgtaa ttacctgtat ttcatctcca tagtttaata
    19381 aaggatgttt atttaaaaac tcttttgtat aataagtttg ctcatattta ccaaccatag
    19441 gagcagcgca agttttttta gatgggtcaa gttttttctt tctacaatct gtatctccac
    19501 cttcttcttt tctaggacag taagctgtaa attctgctgt aaactctttt cctcctgcat
    19561 aatcttctcc ccctaaatta ctttcttcct taggttcatc ctgtcctgct gacttttcat
    19621 ccataagatt ttgaaaatta agttcaagct caatttgata ttctccattt tgccaagtat
    19681 gtttgtctgt atctatataa aatagtccta caagctttgt ataagaatct ttaactttta
    19741 ctcctctacc agttatacaa cttacatctc cataaccttt aagagaacag cttttttcta
    19801 tcccattaaa ctcgctatca atatctacat cttgattttc ttgttgctga attacttttt
    19861 gcattattac atttacttcc ttaaaaattt cattatctat tttttcgcta atcttgcttc
    19921 catactggtc taccactatt actttatttt ttacattttc catgctctcc gaaaaggtgg
    19981 tatttataat attaaatccc tcttcaaaca taacacttaa tgtaacagtt cctttttcaa
    20041 taacattaaa cttatctaaa ttggcctcta tcatatactt tttctttgtc tttttacttg
    20101 cttctgtata tgcactcatt attgtgtcat aaccatttac tcctataaac atctttgtat
    20161 acttgacatt ggtctttgct attattccaa ctgaaagcct attttcagca aatacttgct
    20221 ttgctatgtc ttcaactaac ttatctttaa aattgtatga cacttcactt tgtgtaagta
    20281 aaaatcccat atctttagat acaaaactaa tttcattact gcttgaatct ttagacctat
    20341 taattatcat tcctctaaag agttctttct catctacata gaaacagact gtactagcta
    20401 ttggtatatc gatttgttgg aaatttacat caaatgatga ttgtattatt gaaaactcta
    20461 gtgtccttga tggagattta taatcacctg accatgatac cttgtctact atgtcagtta
    20521 tgtcatatat acttccgttt tttatgtgta cccaaatctt aataataaaa aatcacctgc
    20581 cttttcattt aattctatca gcagttattt ataacttatt atctataata gttattgttt
    20641 tggagttact tttggtatct ttattctttt atactctttt aaatctaatg taaaatatac
    20701 atctcgagtc ccatctcttt cttcataact aaacccttca attatgactt ccatatttat
    20761 atttgtttcc gtaattgtaa atcttaatat aaaaccttcc ttcatccatt tttctatctt
    20821 atttacacaa tcatatggtg atggaaaacc tgtatagtca cagaaagtat agtctttatt
    20881 tggaaaaaaa cttgatattg aaatgctttt aagagctaca ccaccaaaag ttgctatttc
    20941 acctattttg agtatactag aagagtttac agcagctttt ccattaatac taaaggaaga
    21001 tggaaatact ggaaatctaa atctatcttc agcttgtcta agccacattt ccattataag
    21061 acacccccta actgtgccaa ttggatactt gcaactaatt ttgatgttat cttttctata
    21121 tcagcttctt ctctaatcac tattgtatca gctaacttag ctatgtttat atttccacca
    21181 ttttttctgc ttctatattg atttgcttct tgtttagtta aaaccatttc accttcatgg
    21241 agtctggttt gatagtcatt ataaggaaca tagttcaatc caaatgcatt attacctttc
    21301 ttacctgaat aagctttacc agaatcacca gcctttgttc cagcatatgg attatctttg
    21361 taattttttt tactcttttc cactagattt aaaactgttc ctgtgatgct tccatttttt
    21421 atcttattaa tcatagtttg ccaccattta catatatctt taactatttt acatacttta
    21481 tctaatatgt taaaaaaagc tcccaatatt ggctctataa ttttccatcc agtttccaac
    21541 agaggtccta aggtctccca tacagttttc caaataacac ccagtgtctc tataaaattg
    21601 tttatttgag gtgcatattc acctaaaaaa gtaaaaattc ttgtgataat aggactaaca
    21661 gcttcaacta caggtccgat tagttggaat gctgtagtta ctattgtacc aaatatactt
    21721 attaatcctt gtgcaattgg tgatttattg tttatactat ctagtaaatt atttactaag
    21781 tttactactg gaagtaatga atctattaat gctgtaaaat cgatattttg taatagtgta
    21841 cttcctgcgt taacaattgc ttttggtgca ttccctatca ttgttgctgc atgattttca
    21901 aaacgttctt gcttattttc aaccatcatt ttacgctttt cagcctcatt agaaattcga
    21961 tattgagtag aatcagaaaa ctcttttcca ttttttacac ttctagattg catctggtca
    22021 tatttgttgc caataattat cttaccattc attacattgc ctattttacc agccattttt
    22081 tcagataagc cagctaaatt gcctaatcca ctttctataa aagtttttat tttctttagt
    22141 ccttgaccta aaatatcatc aaaagattta acaatactct tcaagccatt gttaatgctt
    22201 gtttgagctc ctgatattat acctggtatt gtattactca tttctttagc taaccccatc
    22261 tgctttacac ttatgccttc caatgcttca tcaaaagttt tataattttc tcctaccaga
    22321 gtattaagag attccatgtt tccttttgct gcacttaaga aagctgctga tacatcttgt
    22381 tcactacttg tattcatatt taaagctcta acatctacca tagcttgagt tatatctttt
    22441 tcttttttgc caccagtaat attactaatc attttagctt tagttcccat tgcaacaaca
    22501 tcttcagggt caaaaggcgt atcatttgcc attcttacta aactgccata ataatcatca
    22561 acatctattt gattaacttt tccagtattt cttttcatta cactttctag aacaactttc
    22621 tgattttgtt cctgaccaag taaatcaaca ccctttttca cagcgacacc tatagtagca
    22681 ccatttgtca aatcagatat cttccctttt atcgaaccaa gtattccact tgccatatcc
    22741 tttacagcta ttgcaccact ccatacctta cctgcaaata atctcaattt gggaaatact
    22801 ttacttataa tgccacttgc catatctttt atagctatta caccactcca aaaagtattt
    22861 ttaaacaaca ttaacttagg caaaattcta cccatagcac tactcaccat atctttaaga
    22921 ttaatacttc cttgataagg acgtttctct acttgtttta attttcccaa gatactactt
    22981 atcactctct tagtcatatc ttttgctttt attacgatat tccaaatctt gccaccaatc
    23041 ttttttaaat ttccaagtaa tttattttta gttttttcta cttcatcact tttagcttta
    23101 aatattaata taatcataat atttgaatta aactcatttt tcttttgaat caacttatta
    23161 aaataatcaa taatcatagc tatagttatt gctagtctca taatacttcc atttaaactt
    23221 ccaataactc ctgaaacagc atttatgcta gaacttaatt ttgtcatagt tgaacttaca
    23281 ctactagcag tagtttgaac ttttcttaac gaggaattta aactgtttac tctattactt
    23341 gcacttccaa tagaattttt tatgctacta gccattacat tagtggaaag tgaaacagcc
    23401 acagttgctc tcctaaaatt atcagttact gatataattt gattcatttt tggtgtgtaa
    23461 ttatctgtaa ggtacatatt tataccttct tctctcacat ttcccattac ataccacctc
    23521 ctgtcaaagg acacactcca tatccatgtt tcattttttc aacttcttcc tcaatttcta
    23581 aggcgaaaaa agcttcaata agctttaatt cgcctttatc cattgcataa aaaagggacg
    23641 gtcttatacc ttttttcttc caataatagt acattattgt agtcctggta tccgtcccta
    23701 ttagtttttt acctcttcta tcactgcatt tttaccataa cccataacat cttgaagtat
    23761 tctatataag ttggtaattt cacctggtaa taataatttt cttgctaatt cttttggtgt
    23821 tggagcctta aatttatcca ttagctcctt atttttaaat aaaagctttc catccaaatc
    23881 acaaacacct tctataattg taagcatctg catctccggt aagtctatat ccgttttcat
    23941 attttcatca attttaagac aagtattttg tatttcttca tctttactta ttgttaaagc
    24001 tctacacatt actttaaatt cctgcccaaa tatagttgat aatctcttaa gttcaactat
    24061 agtacttggt ctttctaagt tttctgtatc tgaatttaaa agtaaatcta ctatgtttat
    24121 attttttgat aatccatttt cactcattat tatttgctcc tttatcaatc aaattcaatc
    24181 aactaacttt tttacttatt aactatctga atttattaag tatatactta ctaaatatca
    24241 gtataaaact aaatataaaa ctaaattata tcaagaaaat catactcagt aaatgtgaaa
    24301 ggagcttcta tctctccttt tactccaacc tcccaatcag ctaaagttaa atcatcaaat
    24361 gctatatttt ttactgctat tctttctgct ccataagaat ctgggtcatt taattttgat
    24421 atagcaacaa atctaggttc agaacctctc tttatctttt ctccaattaa cttgtgcatt
    24481 cttgaactaa cgtggtatag agttattgag ccttttcctt tatatcccat atactttgta
    24541 tcagtaccca tttgacctgc tattataata tcttcttttg taaattccat ctttgcttga
    24601 aatttcttta cttctgctac tttgtttcca tcaagccaaa gttctcccca agtaccactc
    24661 attacatttc tagcttccat atttgccata ttaataatcc tcctatattt ctattgataa
    24721 atctatatct tccatagcat caagtacttt tatttttgct tttaaaaata ctttagaacc
    24781 tgtgttagct tcttttattt cttgtaatgt catataagat aaatctactc cttttgattt
    24841 taaatacgat ttttgtgctt caaaatctat ttcaacagta gaatcagatt ctataagtgc
    24901 tgatttttct aattcttcta aataactttt tatagctact atcaataaac atttgttgtc
    24961 ataactgtta gtaacctttc ctatatagtc atctattatc acctttctta tgtcactatg
    25021 tataatatct aaagtgtcaa ctatttttat tttctggaac atttctcctt tttctgctgt
    25081 taattcagtt aaagaattta ctcctctagc aattcttata gctcctgctt ctttaataag
    25141 tataagctct cctttattaa cccttgattc tgcatcaact ttcgtcatct taggtatatc
    25201 gactacatca ctaagtttag tataagttac tgattgactt aaaggtgtac ctgctataag
    25261 tccagccacc ctgcttgtaa actcatcaac actgtatttc ttttctccaa ctaacacatc
    25321 ttctgtagta aaattaatta tcccttcatg atttcctaca acttttccta atacagcttt
    25381 aaccttaacc ttatctatat ctctaagttt aattatccaa ttttttatag cagtcttatc
    25441 agcttctact gcttttggca tacatagata attaaattcc ttagtctcta aaaaatctaa
    25501 tgcagtttga atatctgctt ctccttctat tacataaact aataatttat ttggagtgtt
    25561 aacatttccc atcaaagcta aatttatata ttctttattt tcagcagata aatcaactgg
    25621 tatatcctct ttttcatgta tttcattaag acctagtgcc ttagcatctt taagtaccat
    25681 tgcaattatt cctctagctg aacgttcttt aacagttgta gctagctcct taaatgatat
    25741 gttgatactt ggtaatccta tagccatact atcactctcc taaatttaaa ttgatttctt
    25801 tcatattttc atatactaca tcttcttcat atacactatc cagatagttt agtgtaaatg
    25861 taaagttcaa tacataaatt gactcttctt ttttaatact ttgttctagc tttgttatat
    25921 ttaattttct gtctgttacc tttatatttc tgttaaatag cttatttaat tcatctgaaa
    25981 tttcatacat tctaatactc tttgactgtg gttttggtaa gtattttata gagataacaa
    26041 tatctttgtt attcgtattt aacattacag gtgtacaaat aactgatagt atacttacaa
    26101 aaaaacaaga cccttcagag ccttgcacac tttcatcatc aataaaaata ctttcagtaa
    26161 agtttttttt gagaatctca ataattgttt ctaatatttc cttgtattta agcaaataat
    26221 caccacacta tcactaaata ttcttgctta atctgataat cttactataa atgatataag
    26281 tcatttttaa atatatatct catttattcc tgtcaagtat cttctatatt tttaggtaat
    26341 attattcttt tcaatctatc acctccttca atatggattt ctttcacaat aacattatct
    26401 cacatatttt attgcctatt gttgcaatgg tgtatataaa gtgttgtaaa attgtcctat
    26461 tttttataat atctcactca atttaaatat catctttttc tttaaattgt agcaagatgt
    26521 tctatctaag tgtagtttca tagaaatata tgtcatattg ttttttgtct tactattata
    26581 aaaaagatta aaaaattcca tttctatatc atttagacac gttagagcat tttctatctt
    26641 ctctttttct atttctatat cttctttttc catttttaat tttcttaatc tttcctctct
    26701 ttttaacacc tcattttcca cagaagagct tatgttatat gtgttactag tcttttctgt
    26761 atataccatt gctccacatc cagagtattc attctcacat ctttttatgt ccaagttaat
    26821 attctttatc tttatatcca aatacttata attatgtaat cttttatctg tttctttaaa
    26881 taatatatct tttttgtttt ccatagtact tccccttttt tatatactta ttattagctt
    26941 caaatttaca ataatctctt tagtgcacag ttttttacta atctcacaac tttcaagaac
    27001 atattgttct ataactgtat aaaaaaaact tcttctatat tttgttctaa aatactagaa
    27061 atcttcttag ccataagaag tccaggattt tttatacctc tttctatttg agatatataa
    27121 tcatttgtaa ttccagcctt tctggcaagt tgaatttgtg tcataccttt gcattttcta
    27181 taatatttca agttattttt aaacattgtt ttatacctcc attactatta agaatatttt
    27241 gttcttcctc acttagatta tatagaatta tttgttcttt atcaatagat tttagaaaaa
    27301 aatattcttt tatattttat tagttttatt ttttagaaca atttgttata atctatacat
    27361 aaggtggtgt tataatgttt gccaaaagat taagagaact tagaaaggaa tttggattga
    27421 ctcaaagaga acttggagaa aaagtaggcg tttcccaaag agtcttaggg tactatgaaa
    27481 cagaaaacag atttcctgat gaacacattt taaataaatt ggctgatgta tttaatgtat
    27541 cagttgatta ccttcttgga agaaccttag ttaaggaaaa tattgataca gtagctgcac
    27601 atagaaaaaa tccacatgaa gagttacctg aagaagcaca agaacaactt aatgattata
    27661 tagaattttt actaaataaa tataaaaaaa aataaaccat atgatgagca gtctattttt
    27721 ctgctctttt aatataattt aaaggcaaac atacattcta ttttaggggg atttttatga
    27781 ataatttgga taagcttttt gaattagctt ctcaagaaga gataataatt cattatacca
    27841 cttatattgc aggtgattta gaaggactat atataaacaa acatggtatt aaaattatat
    27901 cattactcag taatttaaaa caaaactcaa aaaaattgac atccatctta gctgaagaac
    27961 ttggacatca tttcactagc cttggatact atgtatcctc ttataacgat tattatacaa
    28021 aaattattat agataagtgc gaaaataaag ccttaaaatg ggcttgtgag ttcttaatta
    28081 ctgaagaaga tataataaac ataattaact ctggcatcac ttgcgtttat gaaatggcag
    28141 acatacttaa tgtggatatt actttctttc aaaaaagatt agaatttcta tcattaaaaa
    28201 aacagtcttt acaacttgga aataataaat atttaatatt aactaatttg ccgtatttct
    28261 acatatttga tccaatttca taataaaaat agtagtaatt ttaactggct taacagtaaa
    28321 atctatatat ttattttaac aaattttggt ttctataaat tcaatattaa tatataataa
    28381 tgatatcata ttatttaaaa ttctaaaaac aaatatatgc ttttgttaag aaaggttgtt
    28441 tgatgaatgt ataaagaaaa gttgtttgat aattatttta agttcttagc actattattt
    28501 tggccaatta tgtggtataa gtggatagta atttcaaatg gtactcttga aaatatgtta
    28561 tttaccatat atgcaatcgt cgcaattgtt ttcatcattt tatattcggt gtttatgata
    28621 aaatataaag acattacaca aatagatttc ttttacagga taagtactct acttgctttt
    28681 atatttacac tttttagctt tttaatatac ccaaagagtt tatttttctt gtatttaaag
    28741 attattttta ctggaatata cctttattat tcaatagtaa aaactcttaa atttaaagat
    28801 gacgaaggtg tagtgggtat tatgagctca cttttattaa ttgtaataac tttgttctac
    28861 taaatttaat tctatttgac ttaatatggc tttaaatatt agacatctac attattttct
    28921 gatattattt aagatggtag tcttatatta ataatgaaat acactatgaa ggagttaact
    28981 attatggaaa aaaaagtttt agcaacagtt ggagaaaaag agataactaa tattgatatt
    29041 gaaaatgcat taaaatcatt agacccttat caagctatgc aatttaaaac tgaagaagga
    29101 aaaaaacact tattaaatga tttagtgaat caagaattat tcttcttaga tgctaaagaa
    29161 gaaaaattag atgaagaaga aatatttaaa ttagaaatga aaaagataga agaaaatgta
    29221 ttaaagcaat ttgctataaa taaagtttta tctagtgtta atgtaacaga agatgaaaaa
    29281 gtaaaattct ttgaagctaa taagagctct ttctctaaac cagaaagtgc aactgctaaa
    29341 catatattag tagatagtga tgaaaaagct aaagaaatac ttgctcaaat aaaatcagaa
    29401 gaaatatctt ttgaagatgc agctcttaaa cattcttctt gcccatctaa agatatgggt
    29461 ggtgatttag gaacattcgg tagaggacaa atggttcctg aatttgaaga agctgtgttc
    29521 tctatggcta aaggtgaagt aagtgaacca gttaaaactc aatttggata ccatataata
    29581 aaattagaag accttcaaga aagtacagag tctacatttg atgaagttaa ggctgaagtt
    29641 gaaaaaagtt tattatatca aaaacaaaat gaagtttatg gaaataagat aaatgctctt
    29701 aatgctaagt acggaaacct tgtaaaatac aacgattaat cataaggtca tatataaaaa
    29761 agctgtcttt tcacaatatt tattagaaaa gacagctttt tatattaaag tttaaatatt
    29821 cagtttcata ttatcatttt tttattgtaa agaactcagc ttaaaagttt aaataagatt
    29881 tttaattatc attagctaaa tttttatatc cacgtattat ctcataacag taacctatta
    29941 catctcttct ttttattatc ccaataaatg tgtcttggtc atctataaca ggaatgaaat
    30001 tttgatttaa agattttata actaaatctt caatatctgc gttgatggaa acaggagaat
    30061 tatccatttt tctatttata tctgtcactg gaacatcttc caatcccttt aagtccagat
    30121 ttaaatcatt ttttaatgtc cataaaaaat caccttctgt tatagttcca acatatttac
    30181 catccttact tattattgga attgcagagt atttgtgata ttccattttt tctagggctt
    30241 gccttatagt gtagtcttca tatatatatg ctacttcact ttttggtgtt agaaagaata
    30301 atatgttcat tacactaccc accgttactg aaaacttata aatattactc aatatttata
    30361 atgttacttt tttgcagtaa ctttctcctt tcttattttc taagtattct taatactata
    30421 cgctgtaaca agcgtttttc ctttgctcat tttaatctta ttatgcttta ttaatatttt
    30481 tatatggtct ataagtatca cttattctct ttttagaaaa tttactaaaa attaattgac
    30541 ttaaacttag ccataataat tttactctac actattaatt ttaacatatt tatctcatta
    30601 ttaatgattt tattttaata attttaattt attttgctat taattttatt tacaaaaact
    30661 gaaaaaaccg ctaaatgatt agcggcttat ctctcatatt actagaacac taatatatat
    30721 gaatttttaa ttataatcta actacgttag cagcttgagg acctttagct ccatctacta
    30781 cttcgaactc aacgctttgt ccttcttcta atgttttgta tccatctcca gttatagctg
    30841 agaaatgtac gaaaacatct ccttcacctt ctacagatat gaatccaaat cctttttcgt
    30901 tgttaaacca tttaactaca ccagttttca ttaataaatc ctcctaaaaa aacacaaata
    30961 tttagctact cttttctgta accttattac tactctatca tatatagcta tgtttgtcaa
    31021 ttattattat ttttatacat taataatcaa tttttttact ttataatttt aaatgattat
    31081 tatcttttac actttatttt tctttataat tttttaataa tttattttta ttatatggaa
    31141 tcttatggac ttatcactcc agcctaaatc ccatggcacc aacattctat cagtattatg
    31201 gcaagttacc aaaggatatc ctcttgagtc taatcctgtc actgtcgaaa catgtgttat
    31261 tctgcctttc ttctcataac caacaaaatc ccctggacgt aaattataag cttctttgta
    31321 tacttcttca taggaacctt tagcaattaa tgaacctctt ccactataca gtgcataatt
    31381 tttaaaagct tgtgcattta cccatgcttt tgtacctgct ctctcgctat aattccaaat
    31441 tgagttcttt ttgaaccttc cactttcaaa cattatctgt gatgcaaagt ttgcacaatc
    31501 tccaccttct ggattataat ccctatagtt tggattaaat ttcataccat gttcttcatc
    31561 agctgcggct ccacagtatt tatgtgcata atctatagct tttttttgtt catctgtaag
    31621 ctgtaactct acaccatctt gttgaccaat ataatttctt atatcatcag attttatatt
    31681 ttctaaattt aaagagtctg caaaagggtc tgtataccac tctttagtta tgacatattg
    31741 attatctata atttttacat ttagataatg atatgtacct attctaaagg cattttcttt
    31801 gtccttttga ttttcatatg aatacttata ttcagtggat actgaacaga ttataccata
    31861 gaggtctttt tctttttcct ttattttttt aatcttaacc cttgtcttta tgtctttaaa
    31921 ttttacacct tgtttactag accagttttc taaatatttc atctttttta cttcattttc
    31981 ataagcccac agaccaaatt ttttatccgt ttcataaaga ccttttaaaa tattttcatt
    32041 tttctctaat attgccttat ttctgtagtc aaataagttt tcaagcaata gtttatattg
    32101 gtcttttaca tcatcacctt caacatttgc agattccatg actttttctt ccatattttt
    32161 catacttaaa tcattgatta aaaatacacc tgctgtagta gctaaagaca aaaatacaaa
    32221 tgccattata cctttgatgt acttattctt atttttcagt attttcaaag ctacacctcc
    32281 caaatagtac accttacaaa tcaaattaca aagaatattc tgtcacatat agtaattttc
    32341 atatttaata attctctatt ttattatgtc caatattaaa aattaattgt atcaatacac
    32401 tgtttttttt acaatttttt attttataat ctactttttt ctttttttta tagtaatata
    32461 tatgtatata aatattttgg tatcattata attaatacat actaatggag ggttttttat
    32521 gcaaaaaaaa gtcgcagcaa ttaatgactt atctggaata ggaaaatgct cattatcggt
    32581 tgctatacct atactttcag cattaaaggt tcaatgttgt ccatttccaa cggccatact
    32641 ttcaagtcaa actggttatc ctgaatttac tttcttagac tttactgatg aaatggtaaa
    32701 atattcaaat gtttggaaaa atttaaaagt caattttgat agtatatata gtggtttttt
    32761 gggttcaaag catcaaattg agatagtggc taattttata aacgattatc ccaatgcttt
    32821 tatcgtagtt gaccctgtga tgggtgataa tggagttatg tatccaatat tcacagaaga
    32881 aatgagacag gaaattaaag aattagttaa acattcagat ttaactactc ctaatttaac
    32941 agaagcttgt ttcttaactg gaaatgatta taccaaaagc gattataata gagatgagct
    33001 tatatatatt gcaaaatctg tatctgattt gggacctagc aaagtcgtta ttactggtat
    33061 attagaagat gataatatat taaatttggc ttacaataga gataatgacc atgtattctt
    33121 tacatcagtc aaatataata attgttctta cagtggaact ggagatatat tcacatctat
    33181 actttgtggt atgttagtaa ataaacatga tttgggagta gcggttaata ctgcaacaga
    33241 ttttatatat aaaactataa attacacatc tcaatttgat actgatagaa atgatggagt
    33301 tatgtttgaa aactttttat cagatttaac caatatataa aatcaaaaaa actatccatt
    33361 ttataaatac ctataaatca taaaatttag gtatttacaa aatggatatt atttactaaa
    33421 ctattggtat ttcaattgtt accatagcac caccagtttt cacagaattt tcaatacata
    33481 aagtcccatt atgtttttgt acaatattat ttacaatata gagtcccatt ccataatgag
    33541 atttagaatt tctactcaaa tctcctgtat aaaactgttc tgtagctttt gacaaatctg
    33601 ctttagaaaa accacttcca caatctgtaa ctacaaattt taagtattta ttatgtactg
    33661 atacacttat gtatagttta ctctggcttg gtgaataatc tatagcattt gaaattatat
    33721 tcataatagc tctgaataac agttctctat ctatagttat atttttaggc aaattatctt
    33781 gtttataaaa ctccacatct aagtttttag tactagctaa tgcttctatt tgcactaaaa
    33841 catcttttat aaactcactc atactgattg cttctttatt taatatatag ccattctctg
    33901 tttttgatat atctattaaa gtattaatat atttttctat ttgagtgaca ccctttgaga
    33961 tatattccat gtattctaat aactcaggat tttcatttat ttcaattaat aagtcagtat
    34021 ttccatgtat aattgttagt ggtgttttta tatcatgtgc cagtgcagat atctgttcct
    34081 ttctattttc ttctagcatc cattgttttt ctaaagaatc ttttaacgcc aacttcattt
    34141 tatccataga aaaaaggaca ttattaattt cataaatttt actagattct acaacaaaat
    34201 ctaaatcctg ttgttctatt ttttctgtag tattttttaa aagctccatt tcaatattaa
    34261 atttcttgcc aaatactttt gataatataa caatttcaat aaaaaatatt atactaaaaa
    34321 taatcatacc caatgtctct ggttttggta aatatgctct taaaattgga gaactatact
    34381 ctgatactat tttgtatttt attataaaaa cttcatcttt cttaaaaaac tttatataac
    34441 tttctgaata tgcatgcctt tcttcaatat ctctcattaa attccatact tcttttgatt
    34501 cttttttatt aaaggtccct gaaattaaat tcccatccaa agtataaacc ccatactcat
    34561 atatacttgg aattatatct tcagttactt tttcacttga aataatctgt tctttatatt
    34621 tattaaattg tttttctgca taattagctg gtaaaattac acctgatgat aataatacag
    34681 aaaatgataa cattaaaagg agtacaattg aaattgtcat aataaagaat aaagataaat
    34741 attttataaa aatagttctt aacttttcaa cttttttgtt gttagatttt ttacctccaa
    34801 gcatttgtac cctgtcctcc aatagtttaa ttaattcata attaataccc aaccagtttt
    34861 ttatttgatt attttatatc tttttattgc taagttatat tttaacataa taatataaat
    34921 aaacagtcaa tttttactct attcttctga ttttttacct tcccatttag aaaaccaaat
    34981 acataaaatt acaaatgcta aaattgttac aaaaccacaa attaaacctg ttgattgata
    35041 tgcttttatt gattcaacct ttatatattc aaccgtttta gaacttgaaa aagacgacca
    35101 aatagttata aatctaacac cccatgcaca aggaaaatac tgccaaatac cagaacccaa
    35161 tccagttaat aataatgctg aaagtaacat ttcaactata cctactccaa ttgatgttcc
    35221 ttttccgaac cttaaactta agaataaatg taatatgtat tcaaataaat tacacccaat
    35281 taaaattaga attgaaatca tataaaagct tgatgtaaag taagccttag atataccaaa
    35341 tttaaaacct aatacagaca ctaatgttgc tacaaaacca agacatacta agattatata
    35401 tttacttata agagtaagat attttggatt agatgttgtt aacatatgtt tataattccc
    35461 tgcataatat tcttgttcaa caaacattga acacacaaca cttgacaata aaggaaaagc
    35521 tattgctata acttgtaaat atgctactat ctttagtata ttatcagctc ctgaaacctt
    35581 ataataccat aaaaacaatc ccatccccat taaagcaatg caaatgtgca ttagtattat
    35641 aaaatttctc tttaatttta ttaaatcaga acaaaatatt cttattaact gtctcatatt
    35701 acttcacctc tctattattg aaccaaaccc ttgtagctaa agcaaataat acaaataaaa
    35761 caatagatat taatactcct ggcaaaataa cagcattatt taaaaattca cttccctctg
    35821 gtacaggaag accatttggc agaatatgca atacaacagc cattaatctt gaaggaatcg
    35881 aatatggaaa catataccac atttctgtag ttgagaaaac agcaactcca acaatagtaa
    35941 agaacatatt tgttagaact gtaacaaaca taccaaattt tgaagctaaa aacatacaaa
    36001 gaggaatctg ccacataaat agtaaaataa ttaatatact tgcaatgata ttttgaccta
    36061 ttgaaatagt ttctttatag attatcccaa caaaaactac acctattgca gaaataattg
    36121 atgctataaa acaacaaata atacagtaaa tcatttttcc agtccaaacg tctttcattt
    36181 caattggcaa tggtaaaata ccacgatatt tacttttttc atcttttaat actattaaag
    36241 aagatattaa tgttaatgct cctggaagaa atgtcgtata ccaccagttg aaagcagcat
    36301 tttgccctcc acctagtcca cacaaaagta tacaaaacac tggaactatc catatcaact
    36361 tcattccaaa agttcctttc atcttttgaa attccactct aataaatctt tccatattac
    36421 tgccctctct ttctattttc agctacaact ttcataaaaa gttcttctaa gttttgttct
    36481 tttgatactt ctccttgata acccattact ccattagaaa ttattcctat ttcatctaca
    36541 acttgttcaa cttcagacaa gatatggctt gataaaataa ctgtaattcc ttgttttgga
    36601 aaagaacgaa tcaattctct aagttcttca ataccaatag ggtcaagacc atttgtaggc
    36661 tcatctaaga ttaaaagttt tgggtgattt aataatgcaa tagcaatacc aaggcgttgc
    36721 ttcatgccta gagagaattg acctgaacgc tttttaaagg tatctcttaa atctacagtc
    36781 tctaaaactt cttcaatgcg agagtcagga agaccaagta aagtagtatg gacttttaaa
    36841 ttttctcgtg ctgttaaatt ctcatatata gcaggagact caataagaga accaatattt
    36901 gataaatcct ttctagtcca tctatgtccc tcaaacatta tttttcctga agttggattt
    36961 aaaatcccag ttatcatttt taataaagta gatttcccag ctccatttgg tcctaataat
    37021 ccatatatag aattagattt aattgctagt gaaatgttat caactgccat ctgctcctta
    37081 aattttttag aaactccttt tacctgcaaa atataattac tcataatttt catcctttct
    37141 tttgtcaaac ttatttacaa gaatcattct aaatcataaa tataaggatt ttataagaat
    37201 taaaaaacaa aaaaagatgt gttaaaaaca catctttttt tatcttaatt ctattaattt
    37261 tccattgtag tatatctcta cttcttcaat ccaaatgcta ttatcttctt cttggatgac
    37321 atttttaata atattattta tagtatatat tatattttgt tcattcaaat aatcatcttt
    37381 ccaagaaact ctactttttt tatctggttc tatcaagttt attttaacaa gattatcttt
    37441 ttccttaact tctacttcca tgtctaaacc attaaaatac tcatttgata atgtatctaa
    37501 gattaatcgc agtttatcct taagtgaact ttcttcatca atctgtaagt actttactat
    37561 tttctcatcc tttgaaatta tctctatagg tattttagta gtttttttag aattaatcat
    37621 tttactagtt gtttcttctt ttgcatcttc atccttcttt acaactgctg attctttaga
    37681 atcaactagt tcaactgcac tgatgctata atccatacat gccgtactca ataaagtagt
    37741 agcaattatt aaaaaactta ctgctatttt attcataagt atccctctcc taattctctt
    37801 tcgatactta ttataccata gtatagatta tatttttata cttttaaatc aatttataaa
    37861 ttattatagt aaattattta caattctaag ctatttgaat ttatttttat agtatattct
    37921 ttctctaaat tcgacagatt tttattcaaa agccctttta tttccctcgt atttctatac
    37981 ttgttgctat gttcttcatt atgttcataa tcatatgaat cattgtcata ttcttcatca
    38041 ttattaaatt cttcaattgc ttcttttgac tttatatcac aattaaattt atacttattt
    38101 actggtatat caagtcttac cttagaattt ggaacttcta tgtccaggaa cttagcaatt
    38161 ggcatatcag agtacaagta aaattcactt tcataatttg aatttctacc tattttctct
    38221 tttatattta cattttcagg tatatactct tctatattat tgataaatac atctccattc
    38281 tctgaagata tatccaatga ctctgattct atgtttacat ttttaacgcc taaaattgac
    38341 tttgttgata gatgaagatt gctattagat attaagttta agttttctaa tctatttact
    38401 ttattttctg ttatagctct gctaaaacct ccagaagaag ttttatatgt tatattattt
    38461 gaatctacat catcagatat aaataagtac gaacttatac ttgatgcatt tatatctaca
    38521 ttatttggta catatattct aatttctcta taatcacgtg aaaacatact atttatagat
    38581 gtatttaata agtggtcgaa acttctaatc tttttaattt tattttcaga ttttctttcg
    38641 ccttttacaa ctaaagtctt atcttgtagg gttacactgt atttgtaaaa ttcattattc
    38701 cctcttgttg tcacacgcac atcttcattt gaactttttc ttatcataac atttatattg
    38761 tcaacagcta tatctaactt gtcaatattt actttttctt gataaagttt gttttctttg
    38821 tttaattcag cttccttttc taaaccaaag tttattaggc ttggcataac taaaatccct
    38881 gaccatgttg tccctattat acctattacc aataaaactg cagcaaatat agcaagtttt
    38941 ttattcatgt ttatcttctc ctttcttatt ttctatatct tcaaggtcct tatatatcgc
    39001 ctcaaaattt atactatcat cattatattc atcagtttct tttatgcttt cttcttctct
    39061 attgtagtaa tcattattat atgaattttc attaatgtct ttattcaaat cttcatcatt
    39121 taaagtagct ttttttcttt tttccatctt ttcatttgcc tttatataaa tattttttct
    39181 ctttatccaa tgtatataat tatttagtaa ttgtttacct agttttatgg caaataataa
    39241 aatttgccat gcaagtatct caagccctac aaatgctatg gttgcgaaaa tacaaaacat
    39301 cataacatct ggaaccattg ctataaattt aatcacaaat ggaattaata taacaaaagc
    39361 aattaatgag catactaatg caatagtagc agatacaata gcacatacaa acgcaaatgc
    39421 tggaacaatt agaaataaag ataacactac taataacccc tttataactt gtctggataa
    39481 tcctcctaca ccttcttcta tacttggtgt aagtttatca tttatatttt cttttgattt
    39541 attgtacttt tccttagctt catctttcaa gatagaataa tcctttttta catttgattt
    39601 aaatctacta aatttaccat ttaatatgtc tgtattcatt atttttccat ctatattttc
    39661 ttttgattta ctatactttt cttttgcttc atcttttaag atagagtaac cttttttcat
    39721 atctgattta aatttactaa acttatcatt caaaacatct gtatttatta ttttttcctc
    39781 ttcaatattt ttttctttag tttgtaagat aagttctttt gctatagatt ttggtgagcc
    39841 aagtgatgct attatctcca tatcactctt tccctctata agaccatcta cgaaatactc
    39901 ttcataatct ctcaatatat cattaacttc gctctcagaa aaatcttttt ttagataatc
    39961 ctttaaaatt tcaagaaact cagttttatt caagttcatt acctccctca tcttcactta
    40021 ttaaaatctc aacagccttt acaaattctc tccactcttc catcagattg gataaattct
    40081 cttttcctag tgaagttatt ctatagtatt ttctagcagg accttcatta gattctagta
    40141 tatatgtttc gaaatacaaa tcttttgtaa gtcttcttag aataggatat attgttcctt
    40201 catttacatc tattacttta gagatgttat gtactatctc atatccatac atatctttct
    40261 tagatatgag agcaagtaca caaatttcta aaacaccctt tctaaattgt gtgttcatat
    40321 actccatcct ttctttaaat acacagtatt gtgttttaca aggtactatg ttaaaacttc
    40381 ttatatcctc ataataacac agtactatgt tctgcacaat agttattttt aaatattctt
    40441 gttgattaaa tccttctata aatgtataat actctaatca gatatatttt atgaaagtgg
    40501 tgataaattg gctaaaatga aagatgataa aacaatatta atcctaactg ctcaatttgg
    40561 tgctggacat ataagtgctg caaaagcagt caaagaatgt attattgaaa aatatagtaa
    40621 ctataatgtt gttatacaaa actttataaa tgcaagtatt cccatgatga ataagcctat
    40681 ggtaaaactt tatgaaaaca atacaaaata tactcctgga ttatataatt actattatta
    40741 ctttaagaaa tcgtttgatt caagacatga tttatctcat aaattatata cacctaaact
    40801 ttctgagtat attgctgata taaatccaga tttgattatc tctacatttc cactagctgc
    40861 tgcttgtgta aataacttta agataaaaaa tcctgatata aatataccta cacttacagt
    40921 tataacagat gttgtagata gtatggaatg ggtttttgaa aatacagatt tgtattttgt
    40981 tccttctcct gaaataaaaa atagattttt tcagaaagga ataaatccag actccataaa
    41041 ggttactgga gttcctgttg ataagagatt tcaaattgaa agtaaagaac tatgttgtga
    41101 taaatataga ctattacttc taggtggagg aagaggtcta tttgatatag atgaggattt
    41161 tatgcattgg atagatgaat ttattgaaga acatagtaat tctatagaaa ttacaatagt
    41221 tactggtaaa aacaaaaaat tatatgacaa tctaactcat aaaaaacctc taaaaaatat
    41281 taaagtactt ggatttgtaa acgacatgta taatctaata agagaatgtg acctaatgct
    41341 aactaagcct ggaggagcaa ctatatttga agctattcaa tcacaaactc cagtactcgt
    41401 aaaaatgcca aaagttggac aagaaattga aaacgctaaa tttataatag ataagggtct
    41461 tggcatgatt tatagtgatg acctagacct taaaaacata ttttataaat tagtatcaaa
    41521 tgaatttgat tctataataa actttatgaa aaaaaattta aaagaattta aaacagtcat
    41581 tcatccagag aaaattgctg attatatatc agaattaata gataatcatt acagttaaat
    41641 tgtactatta aaaaatttca aacataaaat taaaggcaat caagtatgct tttacacttg
    41701 agaatttact catagtgttt aataatactt gattgccttt atcatatcaa aatcaaattg
    41761 ttatattagt atatattttt cttgtactgc atatacagtt gttgtatttc ttccatactt
    41821 ctctttaatt ctatttgtaa atctgaagca gtctggtaat ccttattttc tatagcttct
    41881 ctcactctag tgaatagtga ttctctagtt aaagtatcct tcataatatt gaactttaaa
    41941 tccgatattg agttccaaac tacctcatca agcgcatcca tattttcttt tgtgtgtatc
    42001 ttgacaatac ttcttatttt ttgaatccca gcctctatta ttctagtttt taatttcttt
    42061 tgccacttat ctatagctcc aatcttaaat gattctatta tactatcagt aaatacatca
    42121 ccttgtttta aagattttaa ttttgaagca tatatttcta agtttttcat attttcataa
    42181 actgtagccg gtggtattcc aaatcttgca tttctttctt ccaaactata atgttcaaat
    42241 acatcatttt catctctata tgctctatct ttttctaaat agaaactttc ttcacctaca
    42301 tttttagata actctttttc aagttctttt gtactaagac cactcttagc tgctgctttt
    42361 ataccatcca acattgtttg ataacatcct gctattacta aatacgtgtt agatagaggg
    42421 cttggtgacc ttaactcaaa tcttacagtc tttggatttt tcatatctct tattaatcca
    42481 acaagcactg acctatttct tgatggaatc tcataactat gacctaaaga tgtaactatg
    42541 catacaggag cttcaaatcc aggaactaat ctattaaatc catcattaga agctgttaca
    42601 attgggttta atacttcata attgtaaaga agtcccataa gagccccata tcctagttca
    42661 cttagataat cttcttttaa atcttttggc gcaaatagat tttttatact tccatccttt
    42721 aacttcgcac ttactcctac atgtgtatgt ccaccacttc ctgctactcc atgaattggt
    42781 tttgctttaa atgtaacttc tagtccatgt gatgtaaata catcctctat aacatctctt
    42841 actaataatt cattgtctgc tgcttgtaac ggtgtagaaa acttccaaga aacctctaat
    42901 tgctccatag catggtttgt tcttccatct atactaatag aactttgtat tcctccgacc
    42961 tctttgtgag ccatttcagg gtttacacct agcttttgaa gtataattaa acttttctct
    43021 aaacaagttc ttataatacc atgagttctt ttccagtatt gctcttttag gctttgtgat
    43081 acatataatt tctctaagtc agctttatcc tcaggagtat taacccagaa ctcaagttct
    43141 gtagctgctg ttaacattat ttcctctata tcatctactg aatctattcc tatgttattt
    43201 attacatgtg gatattctct aaatatttca tacattgact ttttaaagta cttatctgct
    43261 ttttgaagta ctcctctaga acatactttt ttattatcat gtattaaaaa tgcaggtatc
    43321 tttaatgttc caacaggtag acctacttct tcgtctatat attccatatt gtaatctaca
    43381 taccaatgac aacttttatc tggcattaaa tctactttcg cattatttaa tgtagctata
    43441 ttataaagct caacacttga accatcagtc tgtatagctg attccaaaaa tttatctata
    43501 tcctccaaaa ataattctac aggaatcttt tcatctgttg catttcctcc taaatcaacc
    43561 cccattaagg aaacaaatct tatattttta ttgcttttta ggatttctct taattcttca
    43621 ttagtgtgtt tatctttctc tattacgtat aatagactat ccatttttcc actctcccat
    43681 aatttttatg tatattttat tttgttaaat acaagataaa tttcattttc cttacattta
    43741 tgaatttttc ttgcaaatta ttcgtgtttt attcgtgttt ttatttatat tattatatat
    43801 caagtataaa aagttcgcaa tatt
//
LOCUS       DAGSRM010000026        43070 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.026, whole genome shotgun sequence.
ACCESSION   DAGSRM010000026 DAGSRM010000000
VERSION     DAGSRM010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43070
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.026"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            201..1277
                     /locus_tag="MJE33_001531"
     CDS             201..1277
                     /locus_tag="MJE33_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBY9647472.1"
                     /translation="MKAQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
     gene            complement(1394..2236)
                     /locus_tag="MJE33_001532"
     CDS             complement(1394..2236)
                     /locus_tag="MJE33_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBY9647473.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLEAYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKNVKYKDVVICEIGSVMGTYTSKGAIL
                     ITAI"
     gene            2420..2992
                     /locus_tag="MJE33_001533"
     CDS             2420..2992
                     /locus_tag="MJE33_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY9647474.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKDDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERIRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLVNHWI"
     gene            complement(3155..3427)
                     /locus_tag="MJE33_001534"
     CDS             complement(3155..3427)
                     /locus_tag="MJE33_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647475.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDVHTHEHK"
     gene            complement(3554..4426)
                     /locus_tag="MJE33_001535"
     CDS             complement(3554..4426)
                     /locus_tag="MJE33_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBY9647476.1"
                     /translation="MYGESKKLSIDNNIFLQYCLIILGMILSTLGINLYLAPAKLLSG
                     GVAGICVILYKLFGINQGISSFLMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLT
                     QDIAKYFPIDDTMLQCIYGGALTGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNT
                     FLFINFFVVCAGAFLFGAKLVMYTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEI
                     SKVIMNKMGRGVTFLEAEGAYTQNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNN
                     VEEVKGGGFKDKFL"
     gene            complement(4722..7829)
                     /locus_tag="MJE33_001536"
     CDS             complement(4722..7829)
                     /locus_tag="MJE33_001536"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBY9647477.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLADKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFNEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
     misc_binding    complement(7922..8154)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8329..8856)
                     /locus_tag="MJE33_001537"
     CDS             complement(8329..8856)
                     /locus_tag="MJE33_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBY9647478.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     gene            complement(8891..9676)
                     /locus_tag="MJE33_001538"
     CDS             complement(8891..9676)
                     /locus_tag="MJE33_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBY9647479.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYEQAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILGLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            complement(9686..9943)
                     /locus_tag="MJE33_001539"
     CDS             complement(9686..9943)
                     /locus_tag="MJE33_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBY9647480.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFDILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            complement(9962..10420)
                     /locus_tag="MJE33_001540"
     CDS             complement(9962..10420)
                     /locus_tag="MJE33_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBY9647481.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            complement(10460..11164)
                     /locus_tag="MJE33_001541"
     CDS             complement(10460..11164)
                     /locus_tag="MJE33_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBY9647482.1"
                     /translation="MGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYNKK"
     gene            complement(11174..11809)
                     /locus_tag="MJE33_001542"
     CDS             complement(11174..11809)
                     /locus_tag="MJE33_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647483.1"
                     /translation="MKKVKYTRLVILGILIYLVFNILVMIISKNVDTLVLKNEKVDAK
                     IHVKGSIEKDANDQSNYLSTYISKEDSNNFEKNQNVIIEYHEQKLDAKVYKIYENNNK
                     IMVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVIDEQSQSV
                     KFVILEGITYENESSVFVDYYKNDINGVTSVNLYDKIILRPNSINTNIRIK"
     gene            12190..13230
                     /locus_tag="MJE33_001543"
     CDS             12190..13230
                     /locus_tag="MJE33_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBY9647484.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLSCLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGLITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
     gene            complement(13693..15048)
                     /locus_tag="MJE33_001544"
     CDS             complement(13693..15048)
                     /locus_tag="MJE33_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HBY9647485.1"
                     /translation="MNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREKI
                     YNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDENDYVKFAQTLDKA
                     AEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMDA
                     VKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGVS
                     GPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSLA
                     PTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIPV
                     SEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGMI
                     NNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPINTNSSELFTQRGGRIPAPIHSFK
                     N"
     gene            complement(15074..15343)
                     /locus_tag="MJE33_001545"
     CDS             complement(15074..15343)
                     /locus_tag="MJE33_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBY9647486.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
     gene            complement(15633..17588)
                     /locus_tag="MJE33_001546"
     CDS             complement(15633..17588)
                     /locus_tag="MJE33_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647487.1"
                     /translation="MKKYEIFIFMSIIVLCFISFPHKVFGQEENCLEDFERWVQENKN
                     NKEEIVYTLSCDMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNITGE
                     GGKEGVIHIENGGSISIGINNIIATDGTALYVEEGGDLHIKSSYESVEKIKANGKDAI
                     GIYTENDIRILNKNIEVNGEHAIGVYSKGDVEIEETSIKAYSSNNESGLLKDNNKLAQ
                     SIISESKKVYIIDDYNELIPSIAEDSGYDIVKCCHRGIDVFSDEIKVSKDDKIENIKF
                     PKYMVLETSSGNRLDIDVEWDFTDYYEKLEKGEDFNITGKFKTEMLNKEKIIINDDVV
                     PSLNVSVIEKKPIDNLELEFQNTKNGYVAVLFYDMPYSASKVFVEYSSDGINWISEEQ
                     EDIRDQAILFFEDFKLRCFRVKVVGGLKEGYSNIVFKPGFIMGGGDNQETPDDEREND
                     DIDGDRGGGGRDDPDRDEDDNNQDGNNGNENNDSNNNQNNNNENNNNENDDSDEKQPN
                     TPKDDVESSTDSNENKNPHDIPKDGNKENNSDKEKGFYNKNSITENDDNYDLSSKSVF
                     GTNEHSFPYRESISNKNQDTNKNKSNISDYDGIINNNYKLESKEYNLKKDIIFLFCIL
                     TPIMIGSILIVNPDTRKSIIKFIKLKK"
     gene            complement(17771..19246)
                     /gene="spoIVA"
                     /locus_tag="MJE33_001547"
     CDS             complement(17771..19246)
                     /gene="spoIVA"
                     /locus_tag="MJE33_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HBY9647488.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIIQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            complement(19418..20712)
                     /locus_tag="MJE33_001548"
                     /pseudo
     CDS             complement(19418..20712)
                     /locus_tag="MJE33_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903280.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(20896..22305)
                     /locus_tag="MJE33_001549"
     CDS             complement(20896..22305)
                     /locus_tag="MJE33_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBY9647489.1"
                     /translation="MKKKIGFWDLVFMNISALFGIRWIAKSTASSFGLGLGSIPVWLL
                     FAFIFFVPASLICAELAATYPKDGGLGEWVKQAYGEKWGFMVSWLNWTSKIFWYSSFL
                     TFLAINIAYMLGNPDLSNNKMFILALSLIIFWLLSLVSTRGMAFGKFFTNTGALGSTI
                     PAILLIVMAFMSVVILKKAPSASIYTIQNIIPKIDANSLVSISAIIFALSGAETTANF
                     ITEMDNAKKNFPKAILTVAVLIGGIYILGSVAITMILPPDKIAASTGILDALAKVAQD
                     LGIGSWFIRIVAFGITLSVLGALILYIAGPVKMLFGNVREGVFPKQLTVTNKYNIPSN
                     AVIVQAIIVSLLLVGTNLLPSVDNIYNVLVTMTALTALFPYVLLFTSYIRLRKTRPNE
                     ERPYSIAKSDSVCINIARMVLVVTVVGILFSTAPVMETLKDNIIYEIEMIGGGLFVII
                     TGLMLWKNYEKKISNNSGC"
     gene            complement(22357..23496)
                     /locus_tag="MJE33_001550"
     CDS             complement(22357..23496)
                     /locus_tag="MJE33_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBY9647490.1"
                     /translation="MSNNMRLERLRKYMNEKSIEIALIFEPDNQFYISGFKAITYSRP
                     IVTVVTQDKIELIVPGLEELHAKEVAKVDNVYVYYEIPEMKEHGISHKHYLDIILNKY
                     PKGTTVGIEKDIVSASFSEYITDKSFAIKDVGSKIFEMRYVKDAKEIEFLKIAGYLSD
                     IGIKGSLENVRVGMSELEFDVAGDNALLKYVSENYPDTYIGFANWTCSGIDRTAQPHL
                     DSNTRILQRGDIVIHSRQVWYENYRAENERTFIIGKPTERQKEVFKIAVEAQQAGLDT
                     IKAGIPARMVDEAARAVVAKYGLELYSNHRIGHGLGLSEHEEPYLRFDNELILEEGMV
                     FSMEPGIYIPGVGGFRHSDTAIVGKNGATIITNYPRSVEELILDI"
     gene            23815..24465
                     /locus_tag="MJE33_001551"
     CDS             23815..24465
                     /locus_tag="MJE33_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY9647491.1"
                     /translation="MTNKELQKKRILMYFIEAAQNIMENEGIENITLRKVADMAGYNS
                     STLYKYFKNLDHLISFASIKYFKDYNLNISRCIENVNDEYKKYIIMWKLFCKHSFDNA
                     QAFYQVFFNLSSDELSYITKQYYDMFPEDLGIHDSDISLMITGESIKERNKILLNNLV
                     DTGYIPAKDLDIINDIIISFYQNLLFLKKNTGKNSDDDELTMKMISSIEYIIKHAK"
     gene            complement(24622..25641)
                     /locus_tag="MJE33_001552"
     CDS             complement(24622..25641)
                     /locus_tag="MJE33_001552"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBY9647492.1"
                     /translation="MEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKINRTK
                     ENTDYLPGVLFPNNITISTDIEKTIKDCKVIVLAVPSQAVRSTCQKIKPFIKEGQVIV
                     NVAKGLEKGTGLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDLKIAQ
                     MIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMTRGIS
                     EMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLKEVKM
                     VVEGITATEVAHDVAEKLDIDMPITNAIYSVIKKGSNPKEVGIELMMRSKKHEMEEVV
                     LGDDI"
     gene            complement(25672..26286)
                     /gene="plsY"
                     /locus_tag="MJE33_001553"
     CDS             complement(25672..26286)
                     /gene="plsY"
                     /locus_tag="MJE33_001553"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBY9647493.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLMCLAVFILVVAITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            complement(26321..27640)
                     /gene="der"
                     /locus_tag="MJE33_001554"
     CDS             complement(26321..27640)
                     /gene="der"
                     /locus_tag="MJE33_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBY9647494.1"
                     /translation="MSRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRIFA
                     EVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAADK
                     EIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLDEI
                     VENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAIDTY
                     FEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTEQD
                     TKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAKTN
                     QRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTDIR
                     PPKITLFINDKDLTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            complement(27674..29002)
                     /locus_tag="MJE33_001555"
     CDS             complement(27674..29002)
                     /locus_tag="MJE33_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HBY9647495.1"
                     /translation="MEVNVKKVNNIISKVYKDSIAEEMGIEVGDLLISVNEQPIHDII
                     EYRFLLSDEYLDVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCMFCF
                     IDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVHTTN
                     PELRQKMISNKFAGKLYGIMKRLADAHIEMNCQIVLCPGINDGKELDRTIKELAQLYP
                     YVNSVAIVPVGITKHRENLVELNIFNDKSASKTIEQIHQIQQKYLEKLGTRFAFLSDE
                     FYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPEKILKREKKVSIATG
                     HSAYEFIQSMADAMMDKFKNLQVNVYEIKNKFFGETITVSGLLTAKDLKEQLEDKELG
                     EALYITRSMLKADEEIFLDNIELNQLEELMRIKIIPCLNEGKDFVDKILK"
     gene            complement(29240..29440)
                     /locus_tag="MJE33_001556"
     CDS             complement(29240..29440)
                     /locus_tag="MJE33_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647496.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            complement(29442..30374)
                     /locus_tag="MJE33_001557"
     CDS             complement(29442..30374)
                     /locus_tag="MJE33_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBY9647497.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLLSQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLVLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGLYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNV
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            complement(30419..31351)
                     /locus_tag="MJE33_001558"
     CDS             complement(30419..31351)
                     /locus_tag="MJE33_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBY9647498.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYGLSFFYCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNGVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKSTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            complement(31406..32695)
                     /locus_tag="MJE33_001559"
     CDS             complement(31406..32695)
                     /locus_tag="MJE33_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBY9647499.1"
                     /translation="MEDLNSIYFFIVMLSIAIVILAVRYTITLRKYLKEFIYVSKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLRSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILQFIGSKDNETANIT
                     TKDDIVYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            complement(32724..33416)
                     /locus_tag="MJE33_001560"
     CDS             complement(32724..33416)
                     /locus_tag="MJE33_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY9647500.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            complement(33431..34192)
                     /gene="pgeF"
                     /locus_tag="MJE33_001561"
     CDS             complement(33431..34192)
                     /gene="pgeF"
                     /locus_tag="MJE33_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HBY9647501.1"
                     /translation="MMKDYINIKNVQDIKEYVNLSGNCEELDEKLNSVNLVITCKNID
                     AKNREDMLKVCKECDLTFENLTSNSQIHSDIVTIVNKDTIGKRRDGDALITNLEKVPL
                     LLFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVPEDLVCIIG
                     PSIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLRCSGVKKEN
                     IINLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            complement(34189..34650)
                     /gene="nrdR"
                     /locus_tag="MJE33_001562"
     CDS             complement(34189..34650)
                     /gene="nrdR"
                     /locus_tag="MJE33_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBY9647502.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIDEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKELDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            complement(34672..34935)
                     /locus_tag="MJE33_001563"
     CDS             complement(34672..34935)
                     /locus_tag="MJE33_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBY9647503.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(35039..35812)
                     /gene="sigG"
                     /locus_tag="MJE33_001564"
     CDS             complement(35039..35812)
                     /gene="sigG"
                     /locus_tag="MJE33_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HBY9647504.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(35904..36626)
                     /gene="sigE"
                     /locus_tag="MJE33_001565"
     CDS             complement(35904..36626)
                     /gene="sigE"
                     /locus_tag="MJE33_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HBY9647505.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSDREKQIMELRFGLIDKGIEKTQKEVAGILGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(36651..37523)
                     /locus_tag="MJE33_001566"
     CDS             complement(36651..37523)
                     /locus_tag="MJE33_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HBY9647506.1"
                     /translation="MFKGFKGGYMVYIEYYVIENLVINYIIISCTSILTKRYNPKKNK
                     LLGASLGGAYSVAYLYPSMNILFTVPFKIIIMSIITLISFRSKSKKDYVHVALVFYLV
                     NIFISGSTYFIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTINI
                     NLLEKSFCCKALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQ
                     EIIGSLDDKTSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDR
                     EYGAIMNPNILPGF"
     gene            complement(37813..39087)
                     /locus_tag="MJE33_001567"
     CDS             complement(37813..39087)
                     /locus_tag="MJE33_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBY9647507.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGDIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEIPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            complement(39371..40528)
                     /gene="ftsZ"
                     /locus_tag="MJE33_001568"
     CDS             complement(39371..40528)
                     /gene="ftsZ"
                     /locus_tag="MJE33_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBY9647508.1"
                     /translation="MLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISVN
                     TDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFVT
                     AGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVDT
                     LITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTIM
                     KDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEVN
                     EASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLDTKPKSSIR
                     SSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            complement(40718..41071)
                     /locus_tag="MJE33_001569"
     CDS             complement(40718..41071)
                     /locus_tag="MJE33_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HBY9647509.1"
                     /translation="MLWVLGGLAFGVVVGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGVSLYYVAVLVFGMRLFNNLSII
                     RRYIISNIMNKKRKN"
     gene            complement(41170..41844)
                     /locus_tag="MJE33_001570"
     CDS             complement(41170..41844)
                     /locus_tag="MJE33_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBY9647510.1"
                     /translation="MKKKIVILGAFILGVFISTYIKSLDPNRVYITLGQTESIESEVE
                     NTKKEIKNLKETLKKNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSTVTGPG
                     ITITMRDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQENNGNK
                     VNLFAK"
     gene            complement(41849..42547)
                     /locus_tag="MJE33_001571"
     CDS             complement(41849..42547)
                     /locus_tag="MJE33_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBY9647511.1"
                     /translation="MKKRFINKSIIIGSIVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKKEKENLQSEVNSVKKDIETYRGKNNEGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEINSTKDLGNSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVSNTYMY
                     LKEDKLYMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            complement(42570..>43070)
                     /locus_tag="MJE33_001572"
     CDS             complement(42570..>43070)
                     /locus_tag="MJE33_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HBY9647512.1"
                     /translation="PYIENVEIKRKLPNKIIISLKEKEIFAVLKDEDNYCYIDKKGNL
                     LEELRGSNESKKDLIVDVDYSIDDNKSIKFKNYKTKENVFKTLNYLKEEGIYRKINYV
                     NLKKESNIEMLTRSNIKILLSNDDNLDYNISRVSKILIDLQNKNTNGGTINLNYGKLA
                     VYSPEG"
BASE COUNT        13454 a         7769 c         4660 g        17187 t
ORIGIN      
        1 agacttaatt tattactttt atagaatttt cacaataaaa agtcatttta cttccgtatt
       61 taatgcaaaa gtagagatta aacttaatat aaaaaatata taacaaattt tttttatatt
      121 tctagaagaa aagtatactc ttttttgttt aataatgcta aaattgattt gtattgaatt
      181 ttgattagga ggaaattatt atgaaagctc aaagattaaa tgctgtactt gaacaaatga
      241 aaaaagatga tatttctcaa atgttagtaa gtgaccctac ttctatattt tatcttacag
      301 gagtactaat ccatccaggc gaaagactat tagcattata cttaaattta aatggcaata
      361 ataaattatt tataaatgaa ttattcccag tttcagaaga tttaggtgtt gaaatggttt
      421 ggtttaatga tactcaaaat ccagtagaaa taataactga acatatagac aaaaatgcta
      481 ctatgggtgt tgataaaaac tggccagcta gattcttatt aaacttaata gaattaggtg
      541 gaggaagtaa gtttgtaaat tcatcttata taatagatac tttaagaatg tgtaaagatg
      601 aagaagaaaa agagttaatg agaatagctt caaaattaaa tgacaaggct atggaacaac
      661 ttaaagctac tgtttctggt gaacttacag aaaaacaact tgttggtaaa ttatctaaaa
      721 tatatgaaga cctaggtact gatggatttt catttgaccc tattataggt tttggaccta
      781 atggagctaa tccacatggt gaacctggaa atgcacttgt gaaaccagga gatgcaataa
      841 tcttagatat aggttgtata aaagataact attgtgctga tatgacaaga actgtattct
      901 ataaagaaat acctgaaaaa ggtagagaaa tatttgaaat agttcttgaa gctaataaga
      961 gagctgaagc tattgtaaaa ccaggtgtta gattctgtga tatagatgct gctgctcgtg
     1021 actacataac tgaaaaggga tatggacagt acttcactca tagaactggt cactctatag
     1081 gacttgaagt tcatgataaa ggagatgtat cttctataaa tacagatact gtacaaccag
     1141 gaatgatatt ctctatagag ccaggtatat atttaccagg tgagttcgga gttagaatag
     1201 aagatttagt acttgttact gaagatggtt gtgagatatt aaacaagcat gacaaagaaa
     1261 tatgtgtagt tggttaattc taaaatcaat ttgataaaat taaaaagcca tataaaaatg
     1321 ttttaaacat ttttatatgg ctttaatttc tcaatctatt atttatttca atccattatt
     1381 tatattgcag tatttatatt gcagttatta agattgcacc ctttgaagta tatgtcccca
     1441 tcactgaacc aatttcacag attacaacat ctttgtattt tacattcttt tccataactt
     1501 cccttacctt ttgagccttt tctgggcaat tagcatgtcc tataaataaa accttttctt
     1561 ctttatcttt aatttttgac tctactaaag taatcagctt tttcaaactg tttccttcac
     1621 ctcttgcctt atctatagga gttacaacgc catctgcaac ttgtattatc gccttaaaat
     1681 ttaaggtatt tataattttt cctgctattg gatttattct accaccctta atagcatttt
     1741 ctaaagtttc aagtgttcca aaaaatggag gattatcttt atatttattt attattgaaa
     1801 caatctcttc aatttctttt ccttctttta ttaatattgc tgcttttaac actaattgtc
     1861 ctattccaat tgacccacta agtgaatcta taacctcaac tttcttgtta tttccttcct
     1921 tttcaaacat atcttttgct aaaagtgcac tattataagt cccagataat cctgaactta
     1981 tattaatcac taatacactt tcttcctgac aatggtatgc ttctaaaaat ttatcaggag
     2041 aaggacatga tgtttttggt agtactttac tctcactcat ttttctatag aaagtttcat
     2101 tatctatttc ctttccagat atataacttt cttctccaaa agatacattt aaaccaacaa
     2161 cttctatgtt gtatttttct attatatcat caggtaagtc acatgcactg tccactatca
     2221 attttatttt atccatatta acctcactat aagatattat tttatttatt cagtcggtcg
     2281 tacgaccgat ttaattatta ttttatatat tgaatgaaaa aaagtcaata gatatagatt
     2341 ctcgctttat tgacaataaa tatccataag cttataatgt tattaatttg ttataaatat
     2401 acatggaaag gtaggtttta tgaaaacaaa taatatgaaa caggctattt tatcatctgc
     2461 aaccaattta atagccaaaa atggagttca aaatactagc cttgctgata ttgcaaaaga
     2521 tgtaaatata agtaaaggta ctttgtatta tcactacgca tctaaagatg atattatata
     2581 tgatattgcg gatactcatt tagaagttat aactagtgct atactaaact gtgtaaaaaa
     2641 tgtcaaatca aaaaattctc aaatagaaat ggttaactta atcttagaga aaatatctac
     2701 tatagaaagt cgtggtaggg tacatatgta tcttatttgt gaggcaatta cttcaaataa
     2761 tgatttaaag gaaagaataa gattaaaata tattgaatgg agaactactc ttaaatctga
     2821 aattgttgaa tcactagaaa aatataatag tagcgaaaat gaacaggatg ctgaatcatt
     2881 ttctttttta ctcatgtcaa tagttgatgg tttagttgtc caaagtttat taaaaacaga
     2941 aaaaatacct tatgaaaata tagcttcttt tttagttaat cattggattt aaacttaata
     3001 cttagataat taataaaagt gaactattta agttatctac tttaacagtt cactttctta
     3061 tttattctca ataattttta cttaaaacat tcaattttta tttaaacctt catggaatca
     3121 caaaataaaa atctcttata aatttaaaat gtttttattt atgttcatgt gtatgcacat
     3181 ctgaatgttc atgaccatgt gtatgtgtat gctcatggct atgctcatga gtacctgaaa
     3241 aaccatcatc atgattatgg tcgtgaccta caccatttac atgctcatgt tcatgcccat
     3301 gactgtgaga atgttgatgt gaatgttcat ggttatctcc tccacagcat ccttctcctc
     3361 cagtcccaca acacccactt gtatgtggat tatgatgatt tcttactaca aatctctctc
     3421 ttaacataat tcttatacct cctagtatat tattgagtac ttcatgtacc ccattaacta
     3481 cagtatacat ctaactaggt ataatctcaa tacatatggc attttttaac atatataatt
     3541 aacatatata atcttaaaga aatttatcct taaaacctcc accttttact tcttcaacgt
     3601 tattgacaga aataaaagct tcactatcca tatcataaat taaatcttta aatttaccta
     3661 tttcatttgc agatattatg caataaatca tctttttctt attttgtgta tatgcacctt
     3721 ctgcctccaa aaaagtgact cctctaccca ttttattcat tatcactttg gatatttcat
     3781 catatttatt tgatattaaa agtatagatt tttttctatc aaagcaatct tttcctatat
     3841 ccatagtaaa agaaattata tacattgtaa ttaaagtgta catcaccaat ttagctccaa
     3901 ataaaaaagc tcctgcacat actacaaaaa aattaataaa taaaaatgta tttttcatag
     3961 gtatatcata ctttcttttc attatggctg ctattatatc aagtcctcca gcagtagctc
     4021 ttgctttaaa agttaacccc atacctatac cagttaatgc accaccataa atacactgta
     4081 gcatagtatc atctattggg aaatatttag caatatcttg tgttagcccc agtgctagag
     4141 aaaatagtaa catattgaca aaacttatta gcaaaaattt attatcaaaa tactttctag
     4201 ctataataaa tattggtatg ttcatcaaga aacttgatat tccctgatta ataccaaata
     4261 atttgtataa tataacacaa attccagcta ctcctccact taataacttg gctggtgcta
     4321 gatataaatt tattcctaag gttgatagaa tcatacccaa tataataaga caatattgta
     4381 aaaatatatt attatcaata cttagctttt tactttcacc atacatcaaa acgcacccct
     4441 ttgcataata aataaaccac cctgcataac acacgataaa tttacaaata aatttatcag
     4501 aagtagtttc ctaaaaatat tattcatcct cacttaaacg tttattcatt aatattctat
     4561 cacatgacat aaataattta tatgatttat atagatttat ttattgtaaa ttttggaata
     4621 ttaaaacatt ttattgaata gataattata ttacataaaa aatccctata gaaatctata
     4681 aactaatttc tatagggatt ttggttaact aactattatt tttatttttt tataactttt
     4741 atacctgtca tatggtcatt taagttttgt tcctctaaag atttatcatc tactctgttt
     4801 atttctaaag ctaaagtttc agacttgata tattcattaa aatggtcaac agccttagca
     4861 atttcatcat caccacagta ttctattact atattatcta atacttcaaa gttattagat
     4921 tttcttaatt gctgtacttt agatataaat tctctagcat aaccttcatt aatcaactct
     4981 tcagttagct tagtatctag tataacaaaa agattgtttt caacagatac attaaatcct
     5041 tccttagcag ttatacctat taatactaaa tccttattaa attcaaaagc ttcaccatct
     5101 aaatcaactg ttaaagtctc acctttttca agtttattag cagtttcatg agcatttaat
     5161 ttacttaatg ctccaacgaa taaattcatc ttagaaccta ataatggccc tgcttcctta
     5221 aagtttggtt ttaaactaaa gttcatatac tcatctaaat ccttagcaaa tattacctct
     5281 ttaacattaa gctcttcttt tattaaatca actacatcac ttattgtatc ttcaaactta
     5341 ccatctacta acactttttg taatggttga cgaactttta ttctttctac ttctcttgaa
     5401 gctctaccta aagtaactaa gtttttagct aaatccattt tttcttctaa tttagtatct
     5461 actaactctt cattacattt aggatattca gctaaatgaa cagatacttc tcctgttaag
     5521 tttctataca tttcttctga catatatggt gcaaatggag ctatagctct acaaagacca
     5581 tgtaacaatt cataagttgt attgtacact gcttttttat cttctgttaa ttcagttgcc
     5641 cagaatcttc ttcttgaacg tcttatatac cagttagata aatcatcatt tataaaatct
     5701 tgtatcattc ttaaagtttt atttaactca aatatagcta aattttcttc tacatctttc
     5761 attaaattat tgaacttaga taatatccat ctatcaagtt ctggtctatc tttatattct
     5821 acaaaaaatt cagttggatt tatatcatct gtgtttgcat ataaagtaaa gaagttatat
     5881 acattcttca tagtacctat aaatttactt tggatttctt ttaatccatc catatcaaat
     5941 ttagttggag tccatggtgg agaaacatat agtaaatacc atcttaatgc atctgctcca
     6001 tattggtcaa ataattccat tggatttact gtatttcctc ttgatttact cattttctta
     6061 ccatctttat caagtacaag gtctggaact aaaacactct tatatggtgc ttttcctaat
     6121 acaaatgttg atactgcaag taatgagtag aaccaacctc tagtctggtc tataccttct
     6181 gatatatagt cagctgggaa caattggtca aagttttcct tattttcaaa tggatagtgg
     6241 tgttgagcaa atggcatagc cccactatca aaccaacagt ctataacctc tgttactctt
     6301 gtcattggtt ttccacattt ttcacatttt aagtgtatat catctacata tggtctatga
     6361 agttcaactg tttctggatt tacatcttct atagcttttt cagctaattc agctcttgaa
     6421 ccaactgaat ctgtatgacc acattcacat ctccatatag gaagtggagt tccccaatat
     6481 ctagttcttg atattgccca gtcatttaga ttttctaacc agtttccaaa tctcttctct
     6541 cctacaaaac ttgggaacca ttttacacca ttattatttt ctattaattt atctttaagt
     6601 ttagtcatct ctatatacca gcttggtttt gcatagtata ctaatggagt ttgacatctc
     6661 cagcagtgtg gataattatg aactactttt tctttgctat ataatttatt ttcagaagct
     6721 agccatttta ttatttcaac atctagccca tcttccatta caaatctacc ttcccaagga
     6781 gtagatgtaa atttaccagt ttcatctact ggttgaataa ctggtaaatc atatctttta
     6841 cctgtgttgt agtcatcttc accaaatgct ggtgctgtat gaactatacc agttccatct
     6901 tcagtagtta cataatctcc tactgtaata aaaaatgctt ttttgtcaac ttctacaaaa
     6961 ggcattaatt gttcatattc taaaccttct aggtctttac ctttcatttc ttctaaaact
     7021 tcataatctt cacctagtac tttatcagct aaaggtttag atacataata tacctcatca
     7081 ccttttttaa cttttaaata atctacatca gcatttactg ttaatgaaac atttgatggt
     7141 aaagtccatg gagttgttgt ccatgctaag aagtattcat cagcatcttt tttcttaaat
     7201 ttagctataa ctgtattagt ttttacttct ttatatcctt gtgcaacctc atgactagca
     7261 agaccagttc cacatcttgg gcaataagga agtattttat gcccttcata tatatatcct
     7321 tctttgttga atttgtttag tatccaccat acagactcta tatagttatt atctaaagtt
     7381 atatatggat tatctaaatc aacttcataa gccattctct cagtcattat tctccattgt
     7441 ttttcaaatg agaaaactga ctctctacat ttttggttaa attcagctat tccatattct
     7501 tctatttgtt gcttactagt aagtcctaac tctttctcta cttgtatttc aactggtaat
     7561 ccatgtgtat cccaaccagc ttttctcttt acttgataac cagacattgt tttatatcta
     7621 caaactgaat ctttaagcgt tctagataaa acatggtgta ctccaggatt tccattagct
     7681 gttggaggtc cttcatagaa tacgaaactt gggtcatctt tccctttttc taaagttttt
     7741 tcaagtatat ttatgtcttt ccaataatca aaaacttgtg cttctgcttg ttttacagaa
     7801 ctgtctacca atggcttaaa tttcgccatg taaatcaccc tttcctacta aatattataa
     7861 ttccaaaatt ttttattctt tcacataaat ttaaatacta aaaaactcgc cccctttaaa
     7921 ccaaagggac gagttataaa tcgcgttacc accctaattc tatatgtaaa ctaaatacta
     7981 gctacatata acactctaat agtataacgg tactaaccgg cacagcttag tagaatttaa
     8041 tcgttcagcc tgcagctcaa gagtgatttt tatctttata aattcattgg ttctcagcat
     8101 acccaattct ctgtaaaatt atctaaagat aaatgtctct atcaacgctt ttaaaaattt
     8161 atctgtgttt atatgaaatt ctataacaaa taatttgaat agtcaatata tttttgaaaa
     8221 atataataat ttctattttt cttacatttt tatcctattt ttaatttttg ttatagattt
     8281 ctcaaactca cacaattatt ctacataaaa aataaacaaa ttacaagctt attctaaagt
     8341 tgtagcagct tcatcattac tataattata cactgttgtt ccttcaaatg cgtttacaca
     8401 tttttcatct acatcataaa aaatctcatc aacacttctt atttcatctt ctaacagtga
     8461 tttaaattta tttctaaata tcttaaattc tttgactaaa gcatcatact cttttcttat
     8521 ctctataaca cggttattag cttgttctat aatttgtcta gcttttaaat cagcttcttc
     8581 aacaattatc ttagcctttt tattagccgc gctacaagtg tcttctgctg cactttgagc
     8641 agttattaaa gtagctttta gtgtttcttc aatattttca tacttgttta cttggtcttg
     8701 atataaccct aatttttctt taagagctgt attttcccta catagagatt cataatcttc
     8761 cttgatgata tctaaaaatt catcaacctc ttcatctcta taccctctta aacctttttt
     8821 aaactcttta ttttctatct caattggagt tagcattaca tttcctccca gctataattt
     8881 aaattagtcc ttatacaatt aatcttgctt gaacttttat ttttcctttt ttagtaatat
     8941 ctcctatttg tgttacctta gctcttcctt tgcctctaat agaaatcaaa tcatcgtttt
     9001 ttactatttt agaagttgaa gttatctttt cataattgac atggactttt tctccattta
     9061 tgtattttgc actatcttgt ctagatatat tgtaaatacc acttatcaca caatctaacc
     9121 tatctgaaga tactgtaaat gatacttctt tatattttga agaactatta acaatatttt
     9181 ctctggaaat ttcctttaca attacattat ttcttgaaac cttttctaag tttacaatta
     9241 taaaatcgca aatatcacta cttacaacta cttgacaaaa gctatcatgt ataattatat
     9301 ctccaatttt ttctcttttt atacctagac ccagtattga ccctaagtaa tctttatgag
     9361 atatattttt aaatttgaaa ttcccttcta tttgtaaaaa tctaagagta tcttctatat
     9421 cttcatattc catgtaaaat ggataaataa atacagtgct tctttcagct tgttcataac
     9481 ctccatccac actgtatttt atttcatttg tagaatttaa tattgctaca gcatttttca
     9541 cttcatatgg attaagaaac tccgtagact taacatcata gttttttata caactattag
     9601 ccttgtctat cactttaaac attttatttt tcaagtctat atcctttatg tgatttgtta
     9661 gttttaattt atccatccta tcaccttaga atataaccaa tactaattgt cttaggaaca
     9721 tcaataatac tattgcaatt aaaggagaaa aatctatagg tccatttgta tatttataca
     9781 ttatagacct tattggccct tctattggct ctgtaagatt ttccaaaata tcaaaaattt
     9841 tactttgtct tccatttgga aaccaagtca tcaagctttt tataactatc atccatgcta
     9901 ttatatctaa aagataataa agtgcaattc ttattgttcc catatattaa ccgccttttt
     9961 actatttatt ttgccaaggg aagaatgctt tactttctaa ttcttttttc atatttgcat
    10021 ctatatccac attattagga gctagtataa atattgattt agaaaccttt tgtatacttc
    10081 catctaagac ataaactgcc ccattcataa agttaaatat agatttgcga acttcagaat
    10141 tagtcaatcc ttccaaattc actataactg cccttctttg ttttagatgg tctgctatag
    10201 ttgcagcttc atcataaacc ttaggttcaa ctattacaac tttcatttgg ctagttgtat
    10261 gtaaatttac aattttattt gcttttgcag tactaaagcc actgttcatt tcttcttctt
    10321 gaactatatc gtccatgcca ctttcatatt catcttcaat gtaatcttct tcttcatcaa
    10381 ctatccaatt cttaaattta gatattattc catttgacat tattaacttc ctccttaaaa
    10441 tttattcaaa tttgagtaat tattttttat tgtagtttct ttctccaaag attgatgttc
    10501 caactctaac aatggtagcc ccttcctcta tagcaatttc ataatcgtga ctcattccca
    10561 ttgacaaaac atccatccct acattaggga tgtttagttc ttttatttgt tttgataaat
    10621 ctttaagacc tttaaataca cctcttatct catcttcatc ttctataaaa ggtgccattg
    10681 tcattagacc ctttattttt atatttttat atttttctga cacttctttg acaaagtcta
    10741 caacttcttc tctatcaagt ccatgcttag actcttcttt agatatattc acttgtacta
    10801 agcaatctat ttctctgttt atttgttctg cacgtttttg aatttcttcg cataatgcaa
    10861 ttctatctaa agagtgtatc atacaaactt tatcgattat gtacttaact ttattggttt
    10921 gcaaagttcc tatcaggtgc cagtttaatt tatcacctat aatatcatac tttctagcta
    10981 attcttgggg tttattttca ccaacagaag tgattccttc cctcattgcc tcattaacta
    11041 agtctacatc aacagtcttt gtgactgcca aaagggtaat ctctttgctg tctctatcta
    11101 ctttttttgc agctttatca attttgtttc ttatctcaga caaattatct ttaatagaac
    11161 ccattattat cacctacttt atccttatat ttgtgtttat actgttaggt cttaaaatta
    11221 ttttgtcata tagattaaca cttgtaactc catttatgtc atttttataa taatccacaa
    11281 atacagaaga ttcattctca taagttattc cttctaatat gacaaatttt acactttgtg
    11341 attgttcatc aattacatat acaccacgtt tattgtcttt tgtttttata gaagttttag
    11401 gtatttttaa acattccatc tgattatata ttatatcaaa ttcttcgaca cttgtatcct
    11461 gatttgcaac aatttggtta cttattttta attttaccat tattttatta ttattttcat
    11521 atattttgta aacttttgca tctaattttt gctcatgata ttcaattatt acattttgat
    11581 ttttttcaaa gttattagaa tcttctttac tgatgtatgt agatagataa ttactctggt
    11641 cattggcatc tttttctata cttcctttta catgaatctt tgcatcaact ttttcatttt
    11701 ttaacaccaa cgtatctaca tttttgctaa ttatcattac taatatatta aatactaaat
    11761 atataagaat accaagtatc actaatctgg tatattttac ctttttcaaa gactctcccc
    11821 ctataaaatt actttcatta taagtacttc ttctaaaaaa tgtttattcc ttttttataa
    11881 gctaatattt tacgttctaa taattatttt ttccatatta aatttatttt ttatcaatct
    11941 tcctatacaa gactgcatta tttatatata attgtattat aaagtataag tttaatttac
    12001 attttatacg ttaagcttac tttatatgtt tttatagtta tttttatttt tagatgcaat
    12061 ataaaaccaa ttaagattta aaataacttt tgttatgttg acatttattt aggtaatcat
    12121 ttatgaatta ggataacgtt atcataacaa aagtttatgt cctaaaaaaa atacaaggag
    12181 gattttctta tgtcagaaat tttattagga acaggtttac ttttattggt tctatgttgt
    12241 ttcacaattt ttagttacaa ggccccatat ggtatgaagg ctatgggagc tctagcaaac
    12301 gctgcatgtg ctactttctt agtagaggca ttcaaccttt ctttacttgg tgatgtttta
    12361 ggtatggaat tttttagaag tgttggagct gctaatggta gtcttggtgg agttgctgct
    12421 gctatacttg tgcctttagc cttaggtgta tctccagttt atgcagtttt aataggatta
    12481 tcttgtttag atttttctat attatctgga ttcgttgctg gatactttgt ttctttctta
    12541 attaagttta tagagaaaaa agtgccacct ggagtggatt tagtggttgt aattcttcta
    12601 tgtgcaccgc tatctcgtct tatagcatca aatgtagacc cattagttaa tagtacacta
    12661 ctacaaattg gacaagtttt gatagaagct accaacatta gccctataat aatgggaatt
    12721 atactaggag gtctaataac agtagttgga acgtctccac ttagttcaat ggctcttaca
    12781 tctataattg gtcttactgg tcttcctatg gcaataggag cattaggtgc ttttggttca
    12841 gcttttatga actcaacact atttgcaaaa ttgaaattag gttctaaaaa agatattata
    12901 tctgttgcta ttgaaccatt aacccaatca gacataattt cagctaatcc aatacctata
    12961 tattctacta atttctttgg aggagccttt tctggtatca ttgtagcctt gatgggtctt
    13021 gtaaacatga caccaggtac agctacacct gtggctggtt ttgctataat gtttgcttat
    13081 aatccagttc aacaagttct tataacagct ggtttatgtg ctttattcaa tgtaattgca
    13141 ggatttttag gttcaaaaat attcagtacc tttaaagttc ttactgctgg tgaaatacgt
    13201 ggtactgatg tagaagaaga aattgcttaa aaaacaataa gatttataaa tttatatctc
    13261 aagtagtaga gttttatatt gtaggcaatt ttattaattg caaacgtttt attttttatt
    13321 tatgtttatg attattatgt gtgttgaata tttagataaa tttttattta ttatttttat
    13381 ttagattttt gtttagtttt ttatttggtt gatatattag gaataaaaat tagtatagtt
    13441 tattactagt taagtagcat aactttttag atacataaaa aattttatat taattagata
    13501 ttattaaaat aaaagcgata catgaatttt taaatgtcca tgtatcgctt tatttttgcc
    13561 ataatcttaa caactgattt ttaaagttta ttatcttaac aaagttcatt atcttatata
    13621 atatttaaat gcataggctt ctttaattta accatctaaa tatttaatta aatattatta
    13681 tatgtaatat aactaatttt taaatgaatg tataggagct ggaatccttc ctcctctttg
    13741 agtaaataat tcagatgaat ttgtatttat aggcattact ggtgctcgtc ctaaaagtcc
    13801 tccaaattct accatatcac ctacatcaca tcctggggct ggtataattc taacggctgt
    13861 agtcttatta ttaatcatac ctattgctgc ctcatcagct atcatggctc caagcgtacc
    13921 tgctgtagta tttcctggca cagctatcat atcaagacct acagaacaaa tagcagtcat
    13981 tgcttcaagc ttatctatac ttaaagcacc tttaataaca gcatcaatca tacctgcatc
    14041 ttcagatact ggtataaacg ctccactaag tcctccaaca tgtgaacatg ccattactcc
    14101 acctttttta acagcatcat ttaaaagtgc taatgctgct gtagtcccat gagtaccaac
    14161 aacttcaaga cccatttcct ctaatatatt tgctaccgaa tcaccaatag caggtgtagg
    14221 agctagtgat aagtctatta tcccaaatgg aacatccaat ctactagaag cttcctttgc
    14281 cacaagttgt ccaactcttg taattttaaa tgcagtcttt ttaactgttt ctgaaacaac
    14341 atcaaaaggc tctcccttta ctttctctaa agctctctta acaacaccag ggccacttac
    14401 accaacattt ataattctat cagcctcacc aacaccatgg aaagctcctg ccataaatgg
    14461 attatcttct actgcattac aaaatactac taattttgca caaccaaaac cttttgcatc
    14521 ttttgttaat tctgcagttt cttttatgat ttctcccatt tctttcactg catccatatt
    14581 tataccacat cttgtagaac ctacatttac tgaagcacat actttattag tacttgctag
    14641 agcttttgga attgacttaa ttagtatctt atcacctttt gtatatccct tttgaactaa
    14701 tgctgagaat ccaccaataa aatctatacc taaatcctct gcagccttat ccaatgtctg
    14761 tgcaaacttt acatagtcat tttcatctgt tgcacctgca ataatggaaa ttggagttac
    14821 tgataccctt ttatttacaa ttgggatacc aaattcacgc tctatatctt ttccaacctt
    14881 caccaaatct ttagcagaac tcataatctt attgtatatt ttttctctag ctttatctcc
    14941 atcagggtct atacaatcta ataaagaaat ccccatagtt atagttctta tatccaactg
    15001 ttcttcatct atcatcttta ttgtttcaag tatatttcct acattcacta ttatcacctc
    15061 cccttactgt ctactacaga ttatgcattg aattaaatat ttcttctcgc tgaattttta
    15121 catccactga taatttctta cctctttcaa ccaagtcctc aaccgttttc tcaaaagaat
    15181 ttaatgattt ctctaaatca actatcataa tcatagtaaa ataatcctgt aatatttttt
    15241 gtgatatatc tactatgttg atattttgct tgtataattc atcactaatt cctgccacta
    15301 tacctggttt atcctttcca attactgtta aaattgcttt cataataata tcctccctta
    15361 tttttttata aatcttatat ttttacaaat tgaactatgt gttaacatat atttcattta
    15421 tttgttagta aaccactttt tatgtatata tttaatatat tttaatacct atacattcat
    15481 ttgtcaattt tttttcaata ttctattatt tttatactat ttagaaaatt catatattat
    15541 atatgttttc tcaacaataa atacagtttt cataataatg aaaggtatta cttagcaatc
    15601 tcatgatttt ctaaataata cctttctaaa aattattttt ttaattttat gaatttaatt
    15661 atactttttc tagtatcagg atttacaatt aatatagaac caatcataat tggtgttagt
    15721 atacaaaata aaaaaataat atcttttttt agattatatt ctttagattc taatttataa
    15781 ttattattta ttattccatc ataatcactt atatttgact tatttttatt tgtatcttga
    15841 tttttgttag aaatactttc cctataagga aaagaatgtt cattggtacc aaatactgat
    15901 tttgagctta aatcataatt atcatcattt tctgtaattg aatttttatt gtaaaatcct
    15961 ttttctttat cagaattatt ttctttatta ccgtctttag gtatatcatg aggattttta
    16021 ttttcattgc tatcagtact tgattctaca tcatctttag gagtatttgg ttgtttctca
    16081 tcagaatcat cattttcatt attattattt tcattattat tgttttgatt gttattacta
    16141 tcattatttt catttccatt attcccatct tgattgttat catcctcatc tctatccggg
    16201 tcgtctctac ctcctcctcc acggtcacca tcaatatcat cattttcccg ttcatcatct
    16261 ggcgtctctt ggttgtctcc gcctcccatt ataaatcctg gtttaaaaac tatatttgaa
    16321 tacccttctt ttaatcctcc tacaactttt actctaaaac aacgcaactt aaaatcttca
    16381 aaaaaaagta tagcttgatc tctaatatcc tcctgctctt cagatatcca attgattcca
    16441 tcgcttgaat attcaacaaa aaccttactt gcactatacg gcatatcata aaataaaaca
    16501 gctacatatc cattttttgt attttgaaat tccagttcca aattgtctat tggttttttc
    16561 tctataacag atacattcaa actaggaact acatcatcat ttattataat tttttcttta
    16621 tttaacattt ctgttttaaa ctttcctgtt atattaaaat cttctccctt ttccaacttt
    16681 tcataataat cagtaaaatc ccattctaca tctatatcaa gtcgattacc actagatgtt
    16741 tctaatacca tatatttggg aaattttata ttttctattt tatcatcttt acttactttt
    16801 atttcatcac taaatacatc aattccccta tgacaacatt tcacaatatc atagccagaa
    16861 tcttcagcta tagagggtat aagctcatta taatcatcaa ttatataaac tttcttactt
    16921 tctgatataa ttgattgtgc caacttatta ttatctttta gtaacccact ttcattgttg
    16981 ctactatatg cttttattga agtttcttct atctcaacat cacctttaga ataaacacct
    17041 atagcatgtt ctccattcac ttcaatattc ttatttagta ttctaatatc attttcagta
    17101 taaattccta ttgcatcttt tccatttgcc tttatttttt caacagattc gtaggatgat
    17161 ttaatatgta agtctcctcc ttcttcaacg tataaggctg tcccatcagt agcaattatg
    17221 ttgtttatac caatacttat acttccacca ttttctatat gtattactcc ttcttttcct
    17281 ccttcacctg ttatattcaa ttcattatca tcaataataa atctcccatg attttttatc
    17341 aatattttat atttgttggt atctattgta aaattactgt cataaggaat ataaaatcta
    17401 aactcttcat ctattaccat atcacaagaa agagtataaa ctatttcttc tttattattt
    17461 ttattttctt gaacccacct ttcaaaatct tcaagacaat tttcctcttg tccaaaaact
    17521 ttatgtggaa aacttataaa acacaaaact attatactca taaaaataaa tatctcatac
    17581 tttttcatct tacactccta gaaattttta gctttgaaac aatcctgtcg aaacatatac
    17641 ttcacccact ataatttttc cacaattatc aattattgtc aatttatata agtatgttta
    17701 ttaattgtta taaataaaaa gctacttact aaagcaaaca gacgtttcac tttaataagt
    17761 agctatattt ttataacaaa atagttataa tattagaact tccttcattt acaatttttt
    17821 gtaaagtctt ttgcatttta actcttatct cttctggcat agtatataat ttactttgta
    17881 attgctcttt tactaagtca tgtagtgact taccaaacat atttgattcc cataaatctg
    17941 ctggttgctc ttcaaatact tccataagat attttatcat ctcttcacct tgattttgat
    18001 ttccaactat tggagaaacc tctgtagata tatctgcttt tataatatga agtgatggtg
    18061 cattggcttt taacttgata ccatattgtt ttccttgttt cattatttct ggttcttcca
    18121 gactaagttc ttctaaggaa ggagctacta ctccatatcc ttttatcttg gcatctatta
    18181 aagcactttc tattttatca tactcatttt taactttaga tagtctagtt atcagactaa
    18241 gcagttgata atctccttca attttaaatc cacttttttc ttcaaggaca ttatagaata
    18301 aatcttgctt tgtctgtaaa tcaatattta taacaccttc tcctagctct acattatcaa
    18361 cacctgtatc ttccaaaaac tctagctcag aaaatccttg tataatacct tctatatccc
    18421 ttattttacc tatatctata atactttgtt ttaatgttgt tattatgcta ctttttatcc
    18481 aatgatttct ttcaagacct tcaacccatt ttggtaagtt gattcttatt tctgtaagtg
    18541 gaaaatcata taatacagct tccataacct cctctatatc ttcttcttcc atttcaacca
    18601 cattcattgg aagaactgga acttcatact tttcttccaa ttcacttcta agcatactcg
    18661 tttcctcact ttttggactt aacgtattta aaaccactgc aaatggtttt ttaagatttt
    18721 tcaattcttg tataactctc tcttctggtt ctacatagct ttttctgtca attccagtaa
    18781 ctgaaccatc tgtcaagaca actatcccta ttgtagaatg gtcttttata acttttttag
    18841 ttcctatctc tgctgccttt tcaaatgtca tagcttcttg tgaccaagga gtggatacta
    18901 atctttgttt tccaccttcc tcatgcccta aagctccttc aactatgtat ccaacacaat
    18961 ctaccattct tacttttaaa gatactgtgt cttttatttt tatttctaca ccatctgctg
    19021 gtacaaattt tggttctact gtcattatcg ttttacctga accactttgg ggtatctcat
    19081 ctcttgttct atccttttta aattcattgt ctatattagg tataaccaac ttttccataa
    19141 attttcttat aaaagttgat tttcctgttc ttacaggtcc aactactcca atatatatat
    19201 ccccctgagt ccttttggat atatcttcgt atatgttatt attcatatta ttcctcctat
    19261 acatctaatc ttattaaatt atatttagag ttaagggtta atattcttaa aatagaaaaa
    19321 taaagaccag caatattaat ttaatttaca tatagaaatt tgtaaatttc aatgtaaatt
    19381 aaattaatat tgctggtcca aatagtagtg tattatatta actgacttta gtagtatttt
    19441 catttgaatt attattcatc tcaataagtt ctttacgtct ttcttttgtc atgttcatcc
    19501 atataaatgt aaacaccaat ccaattatgg ctactatagt aagaactatg aagtagtacc
    19561 tatatccagt tacacccgga aatgcatcca atatcttccc tgctgctaaa ggacaaagta
    19621 cttctggaag atatcctaat gtagcaacta ttcctatagc agttcctgat attgataatg
    19681 aataattacc ttcttcaagt aacgaatagt gtaaaccttg aattgcatac atagatacat
    19741 atattgcaac acatccaatc aatagtatat aaatcataga tgattttgtt ggtgtaaata
    19801 ttacacctaa aagacctgca atcataagta taaatccata agatataatc tttgatgaac
    19861 caactttatc tccaagtaat ccagaagcag ctgatgctac tggtctacaa tactgagcta
    19921 atattgagat tgcagatgca aacaccacag tagctccaaa tgattcagtt gaataaggcg
    19981 taaaataata aaatgacata ttagctgaat atgaacaaaa cattattatt gttattatcc
    20041 atgtatgagg catttttatt actgcaaaaa aggcttcttt atcaaatttt gctgatacct
    20101 ctttttcatt atcctttaat ttgaaaaata ctaatactcc aactattaca tttattgcga
    20161 gtataatact ataatagctg taagaccaga tttatcactt acttttgatg aaatataccc
    20221 aaatagtatc aatactagag atagatgaat tgcattggta attccacgac cactttccat
    20281 aaatccaaaa gcttttcctt gttcattttc agatgctaac attctaacag cttttacaag
    20341 agctggccaa aaagtaagta ttgttgttat tccccataag gcatggatta aaaacacaac
    20401 aatatatgga ggataaagtg ttaatacaag accagatatt cctgtaataa ttaaagaacc
    20461 tgaaagcaat tttctggctg aaatacggtc tgctaaaaaa ccaccaaaaa ggtatgatac
    20521 cattgcaagt agaccataaa tacttcctaa gcttcccatt tgtgtatttg taagattaaa
    20581 tgcttgtgca aaagtatcat aataataatt ctttaaatat ggtaaagcat atataaatgc
    20641 tcctgctaca aagagtatta tcaagacttt gaaatttttc caaatgcttg gtttatttac
    20701 ttcaactgac atacttcctc cttatgtaaa tacctgtaaa ttttcataat aattaattat
    20761 tgcttatttt attgtttatt caatatttag gaatatttat taatatatta ggttaatcta
    20821 tcaataattt atggtgagtt atgagctaaa agtaaactct tttagctcac cataataatt
    20881 atatttttat ttatactaac atccagagtt attagatatc tttttttcat agttcttcca
    20941 tagcattaaa cctgttatta taacaaaaag acctcctcct atcatttcaa tctcatagat
    21001 tatattatct tttaaagtct ccataactgg tgccgttgag aaaagtatgc caactacagt
    21061 tactacaagt accattctgg caatattgat acaaactgaa tcactttttg caattgaata
    21121 tggtctttct tcatttggtc ttgtctttct aagtctaata taagatgtaa aaagcaagac
    21181 atatggaaat aatgctgtta atgctgtcat agtaacaaga acattgtata tattatctac
    21241 tgatggtaat aagtttgttc ctacaagaag tagactcact attatagcct gaactattac
    21301 tgcatttgat ggtatattgt atttatttgt aacagtcaat tgcttaggaa atacaccttc
    21361 tcttacattt ccaaaaagca tctttactgg acctgctata taaagtatta atgctcctag
    21421 tactgataaa gttattccaa atgctactat tcttataaac caacttccta tacctaaatc
    21481 ttgtgcaact ttagctaatg catctagtat ccctgtagat gctgcaattt tatctggagg
    21541 aagtatcatt gttattgcta ctgaacctaa gatatagatt cctcctatta aaacagccac
    21601 agttaatata gctttaggaa agtttttctt agcattatcc atttcggtta tgaaattagc
    21661 agtcgtttca gctcctgata atgcaaatat tatagcagat atagaaacta atgaattggc
    21721 atctattttt ggtattatat tttggattgt atatatcgat gctgatggag cttttttgag
    21781 aataactaca gacataaatg ccatcactat aagtaatata gcaggaattg tagaacctaa
    21841 tgctcctgta ttcgtaaaaa attttccaaa tgccatccca cgagtactta ctaaggacaa
    21901 cagccaaaat attatgagtg aaagtgctaa aataaacatc ttattgtttg ataaatctgg
    21961 atttccaagc atatatgcaa tatttatcgc taaaaaagtc aagaaagaag aataccaaaa
    22021 tatctttgat gtccaattta gccatgacac cataaatccc cacttctctc cataagcttg
    22081 tttaacccat tctccaagtc caccatcttt tggatatgtt gcagccaact ctgcacatat
    22141 aagtgatgca ggaacaaaga atataaaagc aaacaataac caaactggta tagaaccaag
    22201 acccaatcca aaactagagg cagtagattt agctatccat ctaatcccaa atagtgctga
    22261 tatattcata aatactaaat cccaaaatcc aattttcttt ttcactgtaa ttcctccaat
    22321 ttcaaattta ttcagactca tacacaatgt attgacttaa atatcaagta taagttcttc
    22381 aacacttctt ggataattag ttataattgt agcaccattc ttacctacta ttgctgtatc
    22441 tgagtgtcta aatcctccaa ctccaggtat atatatccct ggctccatag aaaataccat
    22501 tccttcttct aatattaatt cattgtcaaa tcttaaatat ggctcttcat gttctgataa
    22561 acctaaacca tgaccaattc tatgatttga atataactct agaccatact ttgcaacaac
    22621 agctcttgct gcttcatcca ccattcttgc tggtatacca gcttttattg tatctaatcc
    22681 agcttgttga gcctctactg caatcttaaa cacttctttt tgtctttctg ttggtttgcc
    22741 tataataaaa gttctttcat tttctgctct atagttttca taccaaacct gtctactatg
    22801 tattactata tctcctcttt gaagtattct agtattggaa tctaaatgag gttgtgctgt
    22861 tctatcaatc cctgaacaag tccagtttgc aaatcctata tatgtatcag gataattttc
    22921 agaaacatat tttagtaaag cattatcccc tgcaacgtca aactccaatt cactcatccc
    22981 aactctaaca ttttctaaag acccttttat tcctatatca gataggtaac ctgctatctt
    23041 caaaaattct atttcttttg catcttttac atatctcatt tcaaatattt tacttccaac
    23101 atctttgata gcaaatgact tatcagttat atactctgaa aagcttgcag atactatatc
    23161 tttttctatt ccaacagttg ttcctttagg gtatttattt aaaattatat ctaagtaatg
    23221 tttatgtgat attccatgtt ctttcatttc tggtatttca taatacacat atacattatc
    23281 aacttttgca acttctttag catgtaattc ttctaatcca ggtactatta attctatttt
    23341 atcttgagtt actactgtta caattggtct agaataagtt attgctttaa atccactaat
    23401 ataaaattga ttgtctggtt caaatattaa agcaatttca atgctttttt cattcatgta
    23461 ctttctcaat ctttctagtc tcatattatt tgacataatg cacctctttc ttaatattca
    23521 gataatttat tcaatacaac aaggatgaat atattatttt aaaattctta atatttttag
    23581 tacttgtcgt atttgtatgt aaattaattt tatttgtgat tattttatca taatcatgat
    23641 tattttgtca atcatgattt tattttaaaa aattttttat attctagtga ttttaaatta
    23701 tctaaaatat aacgagtata cattgtattt tgtaatatta ttcttgattt atggtaagtt
    23761 taatgataaa atcacgatta tataaactat attgattatt tgtaggtgaa taaaatgaca
    23821 aataaagaat tacaaaagaa aaggatattg atgtatttca tagaagctgc tcaaaatatt
    23881 atggaaaacg aaggtataga aaatattaca cttcgtaagg ttgcagatat ggctggctat
    23941 aatagttcaa ctctatataa atactttaaa aatttagacc atttaatatc atttgcatct
    24001 ataaaatatt ttaaagatta taacttgaat atttcaagat gtatagaaaa tgtcaatgat
    24061 gaatataaga aatatataat tatgtggaaa ttattttgta agcattcttt tgataatgct
    24121 caagcttttt accaggtatt ttttaacttg tctagtgatg aacttagcta tataacaaaa
    24181 caatattatg atatgtttcc agaagattta ggtattcatg atagtgacat atctttaatg
    24241 attacaggag aaagtataaa ggaaagaaat aagatactat taaacaatct tgtagatact
    24301 ggatatatac cagctaaaga tttagacata atcaatgata taattatttc tttttatcaa
    24361 aatctattat tcttaaaaaa gaacacagga aaaaattctg atgatgatga attaactatg
    24421 aaaatgattt cttctattga atacataata aaacatgcta aataaaatta aaatatggct
    24481 gtctcaaaat agagataagc cttttttatt gttgaaataa taaaaatata tttaatttca
    24541 tataaaaaag agtgcctcgt aaactaaaaa gttcattttg agacacccct aaaataaatt
    24601 tattatttat tatacaatag tttatatatc atcacctaaa actacttctt ccatttcatg
    24661 ctttttactt ctcatcatta gttctattcc aacttcttta ggatttgaac ctttctttat
    24721 tacagaatat atagcattag ttataggcat atctatatct aatttttcag caacatcatg
    24781 tgctacttca gtagcagtta tgccttctac aaccatcttt acttctttta aagtatcttc
    24841 taaactcatc cctttaccta tgagtatacc agctcttcta tttctactat gcatacttgt
    24901 acaagtaact attaaatctc caatacctga aagacctgca aaggtagaca tattagctcc
    24961 catagcaatt ccaagtctgc tcatttcact aatacctctt gtcataaggg cagcttttgc
    25021 attatcacca taccctaatc cgtcacatat cccagctcca aatgctataa tattttttaa
    25081 tgctcctcca agttctactc caactatgtc tggattcgta taaactctaa gtttagggct
    25141 cataaataaa tcttgtatca tctgtgctat ttttaaatct ttagaagcaa ctactacggt
    25201 agtaggaata tcccttgcta cttcttcagc atgagatggc ccagatagta ttacatatgg
    25261 attttgtggt aattcttctt cacagacttg agataatcta agcccagtcc ctttttcaag
    25321 acctttagct acattaacaa taacttgtcc ttcttttata aatggtttta ttttttggca
    25381 agtacttctt acagcctgtg atggaactgc taaaactatt accttacaat cttttatagt
    25441 tttttctata tcagtactta tagttatatt atttggaaat aatactcctg gtaaatagtc
    25501 tgtattttct ttagttctgt ttattttttt agcttgttcc tcttttcgag tccacatgct
    25561 cacatcatta cctttttttg caagccctaa agctaatgca cttccccagc ttccagtacc
    25621 taatacacat actttctcca tgtttaacac cacctgagta taatatattt actattcttt
    25681 cttttgacca atttttctct ctgtcccatt taataatcgc tttatatttg ctctgtgatt
    25741 atatattact gatgcagtta agaacagagc tactattaaa ccagctttat tttttaccat
    25801 aatcataaat attggtgaac atcctattcc tacaacagaa cccaatgaaa catactttgt
    25861 tatagctacc accaatataa atactgctag acacattagt gttattactg gatttacagc
    25921 tagcattgcc cctagtgaag ttgcaacacc tttgcctcct ctaaatccta aaaccgctgg
    25981 ccaattatga cctgctacaa cacatataac tgccagatat ccagctaaaa gagtatctat
    26041 tccaaccaat cttgctgcaa attctgatat taaaacagcc attacacctt taagaacatc
    26101 acctaaaaat gtcattgctc ctgctctttt tccaagtgtc ctaagtacat ttgtcgaacc
    26161 tgcattacca gaaccttgag tccttatatc aaccccagca attcgctttg caactatgta
    26221 tgaggtagat atatttccta aaagatatgc aactacagca ataattatat aactaaatat
    26281 ctccactgaa tttcccccct ttagttatat tttaagatat ttatttttta tttttttgtc
    26341 tatactcaaa ctttatggaa gttccttcaa atccaaaatt ttctcttatc ttattctcaa
    26401 ggtatctttg atatgagaag tgagttaaat ccttatcatt tataaacaat gtaattttag
    26461 gaggtctaat gtcagtttga gtcccatagt aaatttttaa tcttctacct ttgtcagatg
    26521 gtggttgatt taacattaca gcttctccaa ttacttcatt cagtgctgaa gttgatattc
    26581 tcttagaatg ttcatttgac acatattcaa ctgtatctag tatcttgttc attctttgat
    26641 tagtttttgc agaaacaaat acgattggag catacatcat aaatggaaat ttttctttta
    26701 tatcttttgt gtaattgctc atagtcttat tatctttctc tattaaatcc catttattga
    26761 ttacaaatat gcatcctttt ccttcatcat gtgctatacc tgcaaccttt gtatcttgtt
    26821 ctgttacacc ttctaaggca tctattacta tcaatactac atctgctcta tctacagcac
    26881 tcatagccct tattacactg tatttttcta ttgtctcata tattttactt tttcttctaa
    26941 gccctgctgt atctattaga agaaatttct gtccattttt ttcaaaataa gtatcaattg
    27001 cgtctctagt agttcctgca attggactta caattactct ttcttctcca agtatcttat
    27061 tgagtataga actttttcct gcatttggtt tacctgttat agctactctt attatatctt
    27121 cttcatactc tgtatctaat cctgctggaa aattttcaac tatctcatct aataaatctc
    27181 caaatcccat actattggct cctgaaactg caaaaggcgt tccaaatccc aactcataaa
    27241 aatcgtatat attatcaaat tgactttgac tatcaatctt attaaccact aagattactg
    27301 gtttttttgt ctttctaagc aattgtgcaa tttctttatc agcagctgtt attccagctt
    27361 taccatcaac tataaaaagt attacatgtg acatgtccat tgcaagcatg gcttgatttc
    27421 tcatttgaga taatattata tcttcgctat caggttcaat tcctcctgta tccactaatg
    27481 taaaatattt atctaaccat tctacttcag caaatattct atctcttgta actccaggtg
    27541 tattttcaac tattgatatc ctttttcctg caaatttatt aaatattgta gacttaccaa
    27601 cgtttggtct cccaactaca gctacaactg gcctacttat actcatttta tttctccttc
    27661 catcttttgt tttctatttt aaaattttat ccacaaaatc ttttccttca ttcaaacaag
    27721 ggataatttt tattctcata agttcttcta attgatttaa ttcaatatta tctaagaaaa
    27781 tttcttcatc tgcttttagc atacttcttg ttatataaag agcttctcct aattctttat
    27841 cttcaagttg ttcttttaaa tccttagctg ttaaaagacc tgatacagtt attgtctctc
    27901 caaaaaactt gtttttgatt tcatatacat ttacttgaag atttttaaac ttgtccatca
    27961 tagcatctgc catactctgt ataaattcat atgcagaatg accagtagct attgatactt
    28021 tcttctctct ttttagaatc ttctcaggca atatatttag atactcttca atctctgttt
    28081 ttaacttacg tatcatacct acaccatctt caaactgtaa aaaaccttca tattcttcat
    28141 atccaggcaa ttcactatta gatagtatat aaaattcatc tgataaaaat gcaaatcgag
    28201 tacctaattt ttcaagatat ttctgttgta tttgatgtat ttgctctata gttttacttg
    28261 cgctcttatc attaaatata tttaattcta ctagattttc tctatgcttt gtaatcccaa
    28321 ctggaactat cgcaacactg tttacatacg gataaagttg agctagttct tttattgttc
    28381 tatccaattc ctttccatca tttatacctg gacataaaac aatttgacag ttcatttcta
    28441 tatgagcatc tgccaatctc ttcattatac catataattt tcctgcaaat ttattgctta
    28501 tcattttttg tcttaactct ggattagttg tatgcacaga tatatttata ggacttattc
    28561 tatattttat aatattattt atatcttctt cactcatatt tgtaagtgtg acaaaatttc
    28621 cttgtaaaaa agataatctc gaatcatcat ctttaaaata aagagtttct ctcatacctt
    28681 ctggcaattg gtctataaaa caaaacatac acttatttct acagctttta gcttggtcaa
    28741 ttatcggatt tgtaaattca acacccaaat cttcatcata gtctttttct atctcgtata
    28801 tgtagacttc gccatctttt ttttgtattt ctacatctaa atattcatca cttagtaaga
    28861 atctatactc tattatatca tgtattggct gttcattcac agatataagt aaatctccta
    28921 cctctatacc catttcttct gcaatactat ctttataaac cttacttata atattattta
    28981 ctttttttac atttacttcc aatattttca ccactctttt gtattcatca atgcttatta
    29041 taatgtattt taatatgcaa tttatttatt tattatattt tatgatagca tttttaagca
    29101 tttcatgtca taataacaca ttttaattct aatagtcaat ataagtaaat agctataaaa
    29161 tagattagaa caaataatat tcaaatttaa gtttcttaaa aatttaaata ttatttgttc
    29221 ttaaaaaaat tttataaatt taatgtttta ctatgattaa cgtcccattt gtcatatcat
    29281 gaaccattcc acctacacac tttgacatca acaatgcctt attttgtaag tctttctcat
    29341 catttgcata aaatattgga caacctccac catttttcat attcttatca agtgtaacta
    29401 tagctagggt atattcattt aaacctatat ccattcccat ctcatgcact tcctttcttt
    29461 cctattttat aattttttag tgataagttt tttcctttag agcttgaaag tattgggcat
    29521 gactttacag cttccattac tttttccaca ttcttatcta ctggtaaata agcaatcatt
    29581 atactttcat cctctttatt tcttctaggt aatggtacga atgaaggttc tccataatct
    29641 ccatataaac ctaaccttga atatatattg tatactattg cttgtctttg accggagtca
    29701 aataatattc ctgcattagc ataatcctta tttttaggaa ttatttgtat tcctacacct
    29761 ctattaatat atacttctct atcgcttttt agacctatat ttgttatacc ttttaaatct
    29821 cccaccttta gtatagattc gtccacaaaa ctaatttcag caggcattac atcagctatg
    29881 tctccaatac tttttctagt aagaagtttt tttaatataa aggctaacac taatccacaa
    29941 attgaactta ttaccaaact catcccttca cttattttcg tttctgcaag tattacataa
    30001 tacaatccta ttgttaaaaa agatgtaact atacacatat aatttcttac ttcataggtt
    30061 ctagctatct cttctatgaa tgcagctcct ctctttacaa gttgaacttc ctctaaattt
    30121 tgcaatgtat ttcttctatt tgctctcact tgtctaaatt gttctgctgc tagagataaa
    30181 aaagttatag atgtataaga acgatttata agagctggaa ccaataatgc tcctaatgaa
    30241 gaagcgacga atgctaatat aagttgagat agtaaaacat ttggttgcgt tggatactgt
    30301 ttttggtcta aattcaacat tataattcta gatgttatac caataataac agcaacaaaa
    30361 aatactctat ccattaaatc actcctaaaa tatttaattt gaaattttac atgtaaatct
    30421 acaaagtctt tctagaaaaa tcactctgct ttccttttga tgtttctatg ataggtgtac
    30481 ttttcactac atctatcatg acatctatat ctttgagtat tggcatatat ggtataacca
    30541 cagtactttt ttctaaattg gtcttagata tagcaacaat atcaacctca tctacatctc
    30601 tatctatccc catatgaata aatagattat gtattatagc ttgtctttgc cctatatcat
    30661 ttactattcc aaatgcacct aaatctcttg gtataacttc tatggccaga cattcatttc
    30721 tatacttttc tcttgtactt tctagaccta tgttagttat tattacacca tttaccatta
    30781 aaataggtcc ttcaaaactt atttttgcag gaactatatc tgctatatct gctatactgt
    30841 tccttcttaa aaatcttcta aaaatcaaac caacaatagc tcctgacaca atagctagga
    30901 ttgtgcaata aaaaaagcta aggccatact ttcttgcaaa aacgatataa actatcgaag
    30961 ataccagtgc agaaaaaaga cttatataac ttctagattc atacgtagat gctatttctt
    31021 caacgtaagc gtctcctttt ggtactaatt cttcattatc gatattcttt agtgttattc
    31081 tttcttgttc tgcaagacct tgaaactgtt gtattgctac tgcaaaaaat gtcaaggctg
    31141 caaactcctt atctataagt gcaggtaatg ctatagcacc taacgaagct gaaagcccag
    31201 atataattat ctgttctaaa taatcttgtg gtcttgatgg gtattgtttg tctgtaactc
    31261 ttaaaactaa tcctctacat aatattccta caactaatgc aactataaaa gagtgtctaa
    31321 ataaatttgc attcaataaa ctttcttcca ttttctcagc tccttctctt atatagtttg
    31381 tgcaaaaaac aaaaaaaatt cccttctatt taaggaaatt tttttgtaat attgtaacta
    31441 taaattcgct accaatgcca agttcacttt ttacttttat acatccattt agtgatttga
    31501 ctatatgctt tgttatagcc aatcctaaac ccgttcctcc tacatcccta cttcttgctt
    31561 tatctaccct ataaaatcgt tcaaatatgc gttctacatc ttcttttggt atccctacac
    31621 cattgtctat aactttaatt acgatttttt cttcatcttt taacactaca acttgtacaa
    31681 tcccattatc ttgggtatac tttatagcat tatcaactag gtttaagaac acttgcttta
    31741 tatagtcttt attggatagt atacttattg atttatcatc acatttatat aaaacttcta
    31801 tatttttttc actagctgaa tgttgagtta tttcatatac ctctttaaat acatcatata
    31861 ctacaacatt ttccataaga agagtatctt tattttctac aaatgataac agcaatatat
    31921 catcaatcaa cctttttaat ctatctgatt cgctttctat tatttctaag aatctatttc
    31981 tagtattcac atctatattt tcatttgact ttagtgtttc aacaaaccca cttatagatg
    32041 taagtggtgt tttaagttca tgacttacat ttgcaacaaa atcacttctc atattttcaa
    32101 gtttaacttt tttagtaata tcttctatat ttactatcga acctattatt acgttgttag
    32161 tattctgaag ataaactggg tctactttta tcttatatac gatatcatct ttagtagtaa
    32221 tattagcagt ttcattatct ttagaaccaa taaattgaag tatttgtttt aatatttctt
    32281 cttcttttat tacaacatta atattatgac cttcaactaa ttgatttggt tcactcttta
    32341 tcatttcttt tgcttcatca tttatcagaa gtatatttcc atttacatct atagctaaaa
    32401 ttccatgaga tatactcttt aatatagacc ttaactgtaa atgtttatac tgtacttctt
    32461 ctaaggtttt atccatattc tttatcatat gattaaaatt tctagctaat tcgccaagtt
    32521 ctcctttggc tgatatgtta agcctagcat gatattcatt attacttact ttttttgaga
    32581 cgtatataaa ctcttttaaa tattttctta atgttattgt gtatcttaca gctaatataa
    32641 caatggctat ggacaacata acaataaaaa aatatatact atttaaatct tccacaagat
    32701 aacactccta aaatataacc tttttaatct attttgtatc ctacacctct tatagtctct
    32761 atatattttt ccgatttatc ttcatcttct attttttttc ttaaatatct tatatgaaca
    32821 tctactgttc tagtttctcc atagtattca tacccccata ttttatccaa aagataattt
    32881 cttgaaagta cttttccttt attttgaatt aataatttta acaattcaaa ttccttaaga
    32941 gttaactcta tcttttcaga gcctttggta acttcatgtt ttgataaatc tagttttaaa
    33001 ttacctgtag taagtatatt gttttcttca cctaagctag tcaaattata tctccttaaa
    33061 acagaactaa tcctagctaa caactctttt atactaaatg gcttagttat atagtcatca
    33121 gcacctattt caagcccttc aaccttgtca ttttccatat ttttagcagt aagcattata
    33181 acaggaatat tttttaagtc cttatcactt cttatttttc tcaaaacttc tattccactt
    33241 atatttggta acatccaatc taaaagtatt aaatctggtt taatttcctt agcctttata
    33301 aatccgtcaa atccatcata tgaataacta acttcgtaat ttgacacctc caaattgaat
    33361 tttaatagct caactatatg catttcatcg tctataacaa gcactttagt attcattaaa
    33421 aagctcctct ctattttatc tgtagtaccg ttcctattcg ttctgatgcc ttattatgtt
    33481 ttctataaga gtggaattta tctgctctac aactagtgca taaatttaaa tttattatat
    33541 tttctttttt aactccacta catctaagca tatactcatt aattttccat aaatctaaat
    33601 agtaacttcc ctcttttatt atatagaact tttcatcatt atttgtaaga attgtgttaa
    33661 atttttctac taaatcttca gacacttcat aacaacatgg tccaattgat ggtccaatta
    33721 tacatactaa atcttcagga actgttttat agcattcaga cattaatttt attgttttat
    33781 gacctatatt actaaaagtc cctctccaac ctgcatgagc cagtcctatg gctttatttt
    33841 tagcatctat aattgatata ggtacacagt ctgctgtaaa cagtaaaagt ggtacttttt
    33901 ccaagtttgt tataagtgca tctccatctc ttcttttccc tatagtatct ttattgacta
    33961 tagtaactat atctgaatgt atttgagaat tactagttag attttcaaat gtcaaatcac
    34021 attctttaca tacttttaac atatcttctc tattttttgc atctatattc ttacatgtta
    34081 taactagatt tacagaattt aatttttcat caagttcttc acaattacca cttaaattaa
    34141 catactcttt aatatcttgt acatttttta tatttatata atctttcatc atttgtctcc
    34201 tttttctatc agtatacttt taagttcatt tacaaaagta tttatatctt taaattgtct
    34261 atacacagaa gcaaatctta cataggcaac ctcatctaac tctttcaatt tatccattac
    34321 catctcaccg atttttttgg tttcaatttc ttctataaaa catttattta tttcattttc
    34381 aatatgtaca acaatacttt caatctcgtc aatagaaaca ggtctttttt cacatgcttt
    34441 tagcaatcca tattttatct tttctctatc aaaatattct ctactattat cctttttaat
    34501 gaccattaaa ggagttgttt ctattttctc atacgtagta aacctctttt tacatgattc
    34561 acactcacgt cttcttctga tagattttaa atctgtatgt ctagaatcta ttacctttga
    34621 ttctttataa ttacaataag gacattgcat ctatactcct cctttgttta actatatctc
    34681 aaactcatct aagcttaatt ctgaacctat atttactatt attgtatcac aacctatttt
    34741 aagtatatca ttccaaaata taacttcttc atcccctcct cttgaaaaaa aacttctact
    34801 tccatctcca aaaatggtaa atgagactac cttaccttcg tgaatatcca tgtctatgtc
    34861 tattataaac cccatccttt ttccattttt tacatttata atttcttttt ccattatatc
    34921 agaaactcta atcatattgt actcaccctt tctttaaaaa taaatatgta tcgaagtaaa
    34981 aaaaaatgac ctatataggt catttaaatt aaaataatgt ttacagtcca tggattactt
    35041 atacatattt tctcatattt tttaaagcat ttttttcaat tcttgatact tgtgcttgag
    35101 atataccgat ttcatcagca acttctatct gagttcttcc tttgtaaaac cttaaatcta
    35161 ataccaactt ttctctacta tttaatttct taatagcttc tttaagtgat atttcttgca
    35221 accaattttc atcagtatct tttttatctt gaacctgatc cataacgaat attgcatctc
    35281 cattgtcttg atatactggg tcaaacagag atattgggtc ttgtatggca tcaagtgcca
    35341 ttacaactga ttctacttct aattctaatt cctttgctat ttctgatact gtaggttctt
    35401 tagaatttgt tctaattaat ctttctctaa cttgtagtgc cttatatgct atatctttta
    35461 atgacctact tactctaatt gggttattgt ctcttagata ccttctaatt tctcctataa
    35521 tcataggaac agcataagtt gaaaacctta cattttggct taaatcaaag ttatctatag
    35581 cttttataag accaatacaa cctatttgaa ataaatcgtc tatattttct cctctgttgt
    35641 taaacttttt tattacactc aagactaatc ttaaattccc tcttacaaac tgttgtctgg
    35701 cttcttcatc accatttttt atttgtaata aaagttcctt catttgctta tttttaagaa
    35761 ctggaagttc tgatgtatta acaccacata tttcaacctt attaacttgc atatattttc
    35821 agtcctctct caaaagtatt catctattga aattatttac atttccaatt tttttatact
    35881 ttttaagaag aattaattta gcattataca aattttttca tttctttttg caaccttgag
    35941 attattttct tttcaagtct agatatgtag gactgtgata ttccaagtat tccagcaact
    36001 tctttttgtg ttttttcaat acctttatca ataagaccaa atcttaattc cataatttgc
    36061 ttttctctgt cacttaatct gtcaagtgcc ataaccaaca aatctctatc tatttcttct
    36121 tcaattattt tgtatatttc atcattttct gtacccaata catcagataa taaaagttcg
    36181 tttccatcta aatctatatt taaaggttca tcaaatgata cttctgtttt ctttttatta
    36241 ttttttctta agtacattaa tatttcattt tctatacatc tcgaagcata agttgccaat
    36301 tttatatttt tattcagttt aaaagtatta actgctttta tcaaacctat agttcctatt
    36361 gaaatcaaat cttctacatc tatacctgta ttttcaaatt ttctcgatat gtagactact
    36421 aatctaagat ttctttctat taaaattgac tttacacttt catctgtttc tagcttttgt
    36481 aaaagctcca tttcttcttc tggtttcaat ggaggaggaa gtatatttgc ccctcccata
    36541 tagtatattc cctttggctt tattattttt aatttaatac ctaacatagt aataaagctt
    36601 attattcttt ctttcaatcg taacattcct tgtccccctt aaattttcac ctaaaatcca
    36661 ggcaatatat ttggattcat tattgctcca tattctctat cattaaaatt tgaaatacca
    36721 agtattatat ttcctatctt ttgctcatct acttctatat agtcagcttt taagcctatt
    36781 attatgcttg tcttattact tccagcatgt ttataaggta ttattctaac tctacctgaa
    36841 gttttatcat ctaaacttcc tataatttct tgtgctttct tcatatctat atcttcataa
    36901 ttaatattta atagtttttc tggaataaat ccttctaaca cactagactt aactattatt
    36961 acatcacttt gacttattgg gtcttttaac aaattaccac tatctactaa agccttacag
    37021 caaaaacttt tttctagtaa gtttatattt atagtctttg taagctcctt aatgtactta
    37081 agcattttca aatctttata aatgtatttt aataattcac aactcacata agtacatgca
    37141 attaagaaag atatttttaa gtgacttatt cctgtaaaat aaattataaa ataagtgctt
    37201 ccagaaataa aaatgtttac tagataaaaa actaacgcta catgaacata atcttttttg
    37261 ctcttacttc taaatgaaat taaagttatt atactcatga ttattatttt aaatggtaca
    37321 gtaaatagta tattcattga tggatacaaa taagcaacag aataggcacc tcctaaacta
    37381 gcccccaata acttattttt tttaggatta tatcttttag taagaataga agtacaactt
    37441 ataataatat aatttattac taggttttct ataacatagt actcaatata caccatgtat
    37501 ccccccttaa aacctttaaa catattataa gatagtatgt acaaaatttt atgtcacttt
    37561 tgatggtcga atccaaaaca aattctattt aaaaagaaca atgatatatg tcttatactt
    37621 tatgtcgtca aaagatattt ttttaatgcc aaacaaatag acttttttgt agaatccaat
    37681 atatctaggt agaagttatt cgctctactt ttggtatatt tacaaaaaaa gactacataa
    37741 tttaataata tatcaagtaa atcttttact tatataaaat caaattatat agtctgtaaa
    37801 ttcaaatata aattattgtg ctagtaatcc atttgcatat tttgcagatt catttagtgc
    37861 ttgttctgga gttatgtctc cagctatcat catttgaagt tgtttccata aatattcata
    37921 tatctctact ccatgaggtc caactgtcaa tggtctatac atatgtttat ctcttgtcac
    37981 catttcttca aatcttgggt ctccttggta tttttcacct ttagcaactg gagctctagg
    38041 tatttctttg tggaaagctt ccattgattc tttgcttagc atatactcaa gaagtttaaa
    38101 tccaagttct ttatttttac atgctgacat taatgttaaa tcatctactg aagcaaaact
    38161 tccaactgtc ttatcttcta atgatgttac atatccccag tttaaatccg ggaatgattt
    38221 atcaaaagta cttgtagcag cagaggatag cattatagta aatgcagctt ttcctggtcc
    38281 aaatacactt tcaatcattt cattactatc ttttgataaa gcatcttctg gaatatactt
    38341 tttcatatca tttataaagt ttgcagcttt caagccttct gcattattaa atttaactga
    38401 ttttaaatca tcattatata tatctccacc agcttgccat aaatacggat accagttcca
    38461 gtttaagctt ccaaatactt tagaacccca accttgtgca agaccccact ggtcgatttt
    38521 tccatctcca tcagtatctt ttgttgcttt ttcacagatt cttttaaaat catcccatgt
    38581 ttttggaggt ttttctccta atttttctag tatatcttta ttgtagtaca atacgcctgg
    38641 attagcagct tctataggta aaccataaat accacccatc atttttgcat catctaagta
    38701 tagataatta tctgaagttc ctgatttttc taaataaggt gttaaatctt ctaccgcacc
    38761 catttctata aattgaggaa acatctctgc atacatgtat cctatatctg gaccttcacc
    38821 tgcacttatt gctgttgcat atttttctgc gtagttatcc catggtacta tctgaaattc
    38881 aatttcacag ttattttctt tttcaaattt ttcaaatatc ggcttccatg catctgcatc
    38941 atggtctgtt aatggtgcag accatacaac taatttttct ttaccctctt tgcctttaga
    39001 atcttctttt tttgaacatc ctgcaagaat tgatactgat agaatcccta ccataactaa
    39061 agaaagaacc ttttttagtt taatcatgct taatcccctt tcagacttta atttgtcttt
    39121 ttcctcaaat gtttacattt aattcagatt taatattaac aaataagatt actcttgtct
    39181 aatcataaaa aactatacta actcctataa ctatattttt tttctatacc tattagattt
    39241 aattcatact aaaaacttac tttttaaatt ttatcaatat ttaaaaatat ttttaagtct
    39301 agccacaaac taactatcta aattgaacaa aataaaaaga agtctattga cttctttata
    39361 agcttacata ttatcttctt cttcttagga atgtaggtat ctccatgctc tcatcatcat
    39421 cttcttcaat tatacttgcc tttttaggag gctctatttt ttcctcagca attacttctt
    39481 cttcaatttc ttttacagct tgttttactg ttgtatttaa tgaagaacgt atgcttgatt
    39541 taggtttagt atctaaatct aaggcaccat tttgtaatcc ttcaaatcct gtcgcgatta
    39601 ctgtaatcat tatttcatca cctaaatctt cttttataga agctccaaat ataacatttg
    39661 cttctgggtc acaggattcc ataactaaag ttgatgcctc attaacttca aataatccta
    39721 agttaggacc acctgtaaca ttaagaagta ctcctttagc tccttgtatt gatgtttcta
    39781 gaagtggaga ttgaatagct tgtcttgcag catcaattgc tctagtttca ccactagcac
    39841 tacctatacc catatgagca agtcccttgt ctttcattat agtagtaaca tctgcaaagt
    39901 ccaagtttat taacccttca actgctataa ggtctgaaat tgattgtata ccttgtttta
    39961 atacatcatc tgcaactgca aaagcatcta acattgatgt atttttttgt actatctgta
    40021 aaagtctatc atttggtatc gttataagag tatctacttt tgatttaagt tctgctattc
    40081 caccttcagc gttcttcatt cttatcttac cttcaaatgc aaaaggtttg gtaactacac
    40141 ctactgtaag tatccccatt tcttttgcca agcctgcaac cactggagca gctcctgtac
    40201 cagtaccacc gcccattccg gctgttacaa agaccatatc agctccttga agtaacttta
    40261 ctatttcatc tttactctcc tcagcagctc ttttccctac ttctggattt gctcctgctc
    40321 caagtcctct agttaatttt tctcctattt gaactttata ttcagctttt gatgtatata
    40381 aagcttgttt atctgtattt actgatataa attcaacgcc ttttagttga gcttctacca
    40441 ttctattgac agcattgttt ccaccgccac ctacacctat tactttaatt tgtgcacatt
    40501 cttctaattc tacgtcaaag tttagcatca taaaccccct tctctcatga ttcaaagtta
    40561 tcgtatgtat taattatatc attttcttct tttattttgt taacctttaa gtaacatatt
    40621 ccacaaaaac gtaaattttc ctttttaaat ttcttactta tattgtcaaa tattactctt
    40681 taagtataaa ataatttacg tatatattaa atgctcttta atttttacgt tttttgttca
    40741 taatattact aataatatat cgtctaatta tagataagtt attaaacaat ctcataccaa
    40801 aaactaatac agcaacatag taaagagaaa ctcctaactt ttctcctata taagtcaata
    40861 atatagccaa tatagcatta cctatgaaac cagtaataaa aattacatta tcatagttat
    40921 cttcaaaatt ggattttatc gctccaaaaa tagaatccat tattgcaaga atagctactg
    40981 acatatatat ggaataatcc atagaatatg taaatggaat atagtaacct actactacac
    41041 caaatgctaa tcctcctaaa acccataaca taaatcttca cctctttatt ttactttgaa
    41101 acacaactac attacaatcc tttttattaa ttttttttac tttttcttta tttttttgta
    41161 tttttcgttt tattttgcaa ataaattcac tttatttcca ttattttcct gaacttttat
    41221 attaattcca tatatatcct taagagattt agcatatgat tctggagagt ttatagctgc
    41281 actcaataaa tctatatctc ctatagcttt tattataaat ggttgtccat atgtagtatc
    41341 atttactgta atagtagctc ctgaacattt tatcttactt gttgctagta ctctttctcc
    41401 atttatagaa attgctcttg ctccagcctt ttttaaatca tttaaaaccc tcaaaatatc
    41461 tatatcatgt ataatcaaat cgtttggatt ctgtccgtct tttaactctc tctcactatc
    41521 cctcatagta atagttatgc ctggccctgt aacagttgaa taaccactta actctttaac
    41581 ttcttcaagc tctttttgca taagtgtttg gatagattca tttccattct tttttgcttc
    41641 tttatattca ttcaaagttt ttttgttctt tttaagagtt tcttttaaat tttttatttc
    41701 tttttttgta ttttctacct cactttcaat actttcagtt tgacctaatg ttatgtatac
    41761 tctatttggg tctagtgatt ttatataagt tgatatgaaa acgcctaata tgaatgctcc
    41821 taatataact attttctttt tcatactatt atttcccctc tgctttgttt atatcactat
    41881 gaccatttat ttctatattg tcttctttgg tcacttttac tttggcatta taatatgttt
    41941 ctatttccca gacaatacca tactttatat taagtgccga agccaatgta tcagaatcac
    42001 caattgcttt tattataatt ggttctttta tgacagtgtc attcatataa agcttgtctt
    42061 cttttaaata catatatgta ttagaaacta ttctctggtc gtttatagat atggcacttg
    42121 cttgtgctga atttaattta tttatcattg acaataataa atcataatta tatagtatac
    42181 tatcattttt atcagagttt cctaagtctt ttgtagaatt tatctcaatt tttatgcctg
    42241 ttccttcaac atctgtatat ccagctaatg tttcatattt ttctatttct gacttaagta
    42301 tgtcttgttc tccttcattg tttttgcctc tataagtctc tatatctttc ttcacactat
    42361 taacttctga ttgtagattc tctttttctt tttttaaaga tttaagttct tctgtaagtt
    42421 gctctcctct tttaaaagtt gtcatccctc tattttctat attaataatt ttaagttgta
    42481 aactaacaag aacacccaat acaatacttc ctatgattat acttttattt ataaatcttt
    42541 ttttcaatat atcacttcct tacaattgtc tatccttctg gactatacac tgccaacttt
    42601 ccatagttaa gatttatagt ccctccatta gtatttttat tttgcaggtc aatcagaatt
    42661 ttacttaccc tagatatatt ataatctaag ttatcatcat tagaaagtaa gatttttata
    42721 ttacttcttg taagcatttc tatattgctt tcttttttta agtttacata atttattttt
    42781 ctataaattc cttcttcttt taaataattt aaagttttaa atacattctc cttagtttta
    42841 taatttttaa attttataga tttattatca tcaatgctat aatctacatc tactatcaag
    42901 tcttttttac tttcattaga acctcttagt tcttccaata aatttccttt tttatctata
    42961 taacaataat tatcttcatc ttttaatact gcaaatattt ctttttcttt taaacttata
    43021 attatcttat tgggaagttt tctttttatt tccacatttt ctatatatgg
//
LOCUS       DAGSRM010000027        41872 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.027, whole genome shotgun sequence.
ACCESSION   DAGSRM010000027 DAGSRM010000000
VERSION     DAGSRM010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 41872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41872
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.027"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            286..1380
                     /locus_tag="MJE33_001573"
     CDS             286..1380
                     /locus_tag="MJE33_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647513.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGIFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDKLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPVIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDKHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
     gene            1606..2022
                     /locus_tag="MJE33_001574"
     CDS             1606..2022
                     /locus_tag="MJE33_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647514.1"
                     /translation="MNVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(2204..2773)
                     /locus_tag="MJE33_001575"
     CDS             complement(2204..2773)
                     /locus_tag="MJE33_001575"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBY9647515.1"
                     /translation="MKKEFRKKVIESRKNKSANFVLSNSNLITEKLLQMEDIRNATNI
                     MLYLNFNNEVQTDNLIKKLLSLKKIVSSPITIKENHTLIPTQITDLKEGLRIGAYGIR
                     EPNEKSPTIDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFDSIPKETHDAQLDYIVTETRIITPNS"
     gene            2927..4084
                     /locus_tag="MJE33_001576"
     CDS             2927..4084
                     /locus_tag="MJE33_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HBY9647516.1"
                     /translation="MYLVISKKKAIFLGVILVIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGAAKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKTVELTILREDKQLNFKVKTDQIIEKSIESKVIDNDLGYIEITQFISST
                     YTDFDKALKELKAKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTAAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
     gene            complement(4267..5421)
                     /gene="nagA"
                     /locus_tag="MJE33_001577"
     CDS             complement(4267..5421)
                     /gene="nagA"
                     /locus_tag="MJE33_001577"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBY9647517.1"
                     /translation="MKSFIYADKFFMKHGIKEKGYLSIINGKFGEFQQERPNNTDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNRVEGAKIQGIYLEGPFFTEKYKGAQNEKYFKDPDINILKLWQKKS
                     KNLIRKIAIAPERKGSIELTRYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGHHVSPIAVKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFNVNVKNSVARLNSGSLAGSVLTMDKAVRNIVDWNISTLEDAIKM
                     ATYIPALSCNIDNVCGSIYQGMDADFIVTDNDFNIHETYINGICMYKVNK"
     gene            complement(5504..6229)
                     /locus_tag="MJE33_001578"
     CDS             complement(5504..6229)
                     /locus_tag="MJE33_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY9647518.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRTQGKKPGTK
                     VLSFEVVESTKYFSEKLGITTGEKMIKIMRLRLADDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEIIKVADEEFSAGIISDKEAKLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            6496..7425
                     /gene="pfkB"
                     /locus_tag="MJE33_001579"
     CDS             6496..7425
                     /gene="pfkB"
                     /locus_tag="MJE33_001579"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBY9647519.1"
                     /translation="MITVITFNPSIDRMYRVNNINIGEVQRVVSKNATAGGKGLNVAK
                     VCKILKENPLAMGFLGGFNGEFIKNELRKLDIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNTCRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGMNIESREEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNEDNVYDVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLGEIDKYQDLVEVKKLS"
     gene            complement(7554..8471)
                     /locus_tag="MJE33_001580"
     CDS             complement(7554..8471)
                     /locus_tag="MJE33_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBY9647520.1"
                     /translation="MKNIKMGMIGLGSIAQKAYLPILTKSERFEFVGAFTPNKVKREK
                     VCSDYRIMPFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVSQ
                     GEELIELSTKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVRFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKNFSINTSMHRDSG
                     TKLEQVEILSKGKIQRVKNLNVLEIEEGGNLTLKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPAINGEECIKAQRLLEKIINSVK"
     gene            8942..10111
                     /locus_tag="MJE33_001581"
     CDS             8942..10111
                     /locus_tag="MJE33_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBY9647521.1"
                     /translation="MKMFKLEEQKLKDLGAIITTNEIKQQPELWLETYEIYKSNKEKL
                     SRFIDTISNNHGQFRVIFTGAGTSAYIGNSILPYLKNKNDIRKYIFEAIPTTDIVSNP
                     YDYLKKDIPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPEGELAKMTKND
                     ENNYLLLMPSKSNDEGFAMTGSFSCMMLSAMLIFDSLEDDVEKSYINAIIEMGRNVID
                     RKDEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKISTVYDSPMGFRHGPK
                     SFIDENTLVFEFVSNCLYTRKYDLDVLEEIKRDKIAKFTCAVSVENENNFSGTKFEFK
                     EKYNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
     gene            10160..11011
                     /locus_tag="MJE33_001582"
     CDS             10160..11011
                     /locus_tag="MJE33_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBY9647522.1"
                     /translation="MALVTTTSMLKKAQDEGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVNIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISISKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAIAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDDIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLDEDSEVIDPKKYGLAGLQSVKDFVKEKIKV
                     CGSVNRV"
     gene            11337..13238
                     /locus_tag="MJE33_001583"
     CDS             11337..13238
                     /locus_tag="MJE33_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBY9647523.1"
                     /translation="MRERIILETLKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQDKFEHYLRNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEQLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFRGNRNITEYRNKFIDNLKLANIRSTIKNVLNEHDLEANLTELEEILAQIIILYIRV
                     SMRVVGNASDLKLYTEDLIIDKLLDKIFRDKKYNLNYDEKYYLKELIKLKTKDKKATI
                     KNIDKNKLQDIMFEFFREIDKKYNSNFLEDKEFFNLFYLHIACLIERIKKNHKIINPF
                     SVKISQQYPTVFNLSIQFSKIIENEYHIKISQDEIGFIATHIAVPFEKREEANFNKKY
                     KIAIICSSGGGSAFLINLRLKEIFPNAEIRNFSLLEEKAVIEFAPDLIFSITNLLFEI
                     NAPVILINEILDELDYLDIKESVRFADHIGNISSPKQYILGLFDKNHFRCIKDKAKYK
                     DILDSMSKCIVDEGACSPTYPKDVWERESYLSTIYTNGVAIPHPIEMTGNKNIISVAL
                     IQTDIIYENRVPKIIFMISLIKGNLELHKQISKYLSKIMANKDMVDMLNKSQSYEEFM
                     YKLKIYLGG"
     gene            13241..14284
                     /gene="ypdE"
                     /locus_tag="MJE33_001584"
     CDS             13241..14284
                     /gene="ypdE"
                     /locus_tag="MJE33_001584"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBY9647524.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELKAYSDKIICDGLGS
                     IIFSKIKDESAPNVMICAHMDEVGFMVRSIDKLGMIHLITIGGVKPLAQFVQKVRITT
                     KEGKKIPAVINATYNNGKAENIYADIGAYTEEDVYNLGINVGDMVTYTTSFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELRKENLNCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNKKQIDKGVMLMHRDRTLVPSRKMIDYVMEIADKNNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLENIIRLYTQLILN
                     FDEEQYKHIRNFI"
     gene            14299..14604
                     /locus_tag="MJE33_001585"
     CDS             14299..14604
                     /locus_tag="MJE33_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBY9647525.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDIEAHRIQTELITQDLNEDVESPAMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            14701..15036
                     /locus_tag="MJE33_001586"
     CDS             14701..15036
                     /locus_tag="MJE33_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY9647526.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            15068..16390
                     /locus_tag="MJE33_001587"
     CDS             15068..16390
                     /locus_tag="MJE33_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBY9647527.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGENNPISKFILSNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFISLKVFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPVFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLAVLLYWPFFKIMERQQNNKKQKEETKNIFSKEEEDLLDDLGLDF"
     gene            16400..17470
                     /locus_tag="MJE33_001588"
     CDS             16400..17470
                     /locus_tag="MJE33_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBY9647528.1"
                     /translation="MNNTMERLVSLIYKRGVDALLIKAKNTKRYIGALSGSGVYVLVT
                     KDKNYQILDGRYIDEADKKTTGFIKRIVSQGSYIPTIIELLKELNIKKLAIENQAMSI
                     QEYLSLKNEGFEINLITNELWKLRAIKSKEEIKLIKKACEITDEVFDEVISEIKKDMT
                     ELEVSALIQYHALKKGASGMSFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVY
                     QGYQSDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGTRASDVDKVARKIIDKH
                     GFGEYFNHGLGHGIGLGDGEVPTLNPNSEDILVEGMVMSCEPGIYIPNVGGVRIEDDI
                     VIIDGKGVPLNKTSKEFIILGE"
     gene            17475..18662
                     /locus_tag="MJE33_001589"
     CDS             17475..18662
                     /locus_tag="MJE33_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBY9647529.1"
                     /translation="MKYNFNKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKESILKRASVGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAVVNNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDVKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDAMTSYVEENFEIVYDFFETNIPKAKVMKSDSSFLAWIDVR
                     EVFRNEEESKEFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLNEALNRIKKTYIS
                     MYC"
     gene            complement(18859..19731)
                     /locus_tag="MJE33_001590"
     CDS             complement(18859..19731)
                     /locus_tag="MJE33_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBY9647530.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNILWDGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDTFVTYKLTDNESTLK
                     KYPYSFELIINYTLNDNSIEVEWIVKNTDSKDIFFSIGGHPAFNLPFYEQNDFSKHYL
                     EFKSKNDVEKINLNGSFTDDIIPIGKLKNLQLNPEVFKNDALIYTNIDEVSICNNDGS
                     KYVTISMEDFPLVGIWTPYYSETNSTAPFLCIEPWYGLADSINSNKIYKDKKFINKLS
                     KGKVFTASYNINIH"
     gene            complement(20125..20316)
                     /locus_tag="MJE33_001591"
     CDS             complement(20125..20316)
                     /locus_tag="MJE33_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647531.1"
                     /translation="MDDFSKLKSLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            20536..21582
                     /gene="cobT"
                     /locus_tag="MJE33_001592"
     CDS             20536..21582
                     /gene="cobT"
                     /locus_tag="MJE33_001592"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HBY9647532.1"
                     /translation="MSLLESISKNIYSLDNSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFDTSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFDIIEASNYT
                     YKNMATFDEIDMGR"
     gene            21614..22174
                     /gene="cobU"
                     /locus_tag="MJE33_001593"
     CDS             21614..22174
                     /gene="cobU"
                     /locus_tag="MJE33_001593"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBY9647533.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCFDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPRNWNTYEIYEDIYSIIKEIYKEHETVILDCVTLLVNNLMFEHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIEEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIVG
                     RINQYIASKSNEVYFVVSGIAMKIKE"
     gene            22184..22957
                     /gene="cobS"
                     /locus_tag="MJE33_001594"
     CDS             22184..22957
                     /gene="cobS"
                     /locus_tag="MJE33_001594"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HBY9647534.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLIGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRDKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKVGFVYDIISNNSLWVIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVALITKFIFLLPNIVLIKVLGSIIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSELVYLIYIYLLIFMFF"
     gene            22977..23591
                     /locus_tag="MJE33_001595"
     CDS             22977..23591
                     /locus_tag="MJE33_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBY9647535.1"
                     /translation="MIRLILIRHALTNDNKKGRLSGHINSCISEEGKLQINKITRYLS
                     NENIDKVYTTPSTRTKDTVEKISKLKLLEIEEKEALREISFGDFEGRTFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENNSNLDTKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLEIDGGFAVIDKMNFTDFI"
     regulatory      23658..23837
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            23967..25466
                     /locus_tag="MJE33_001596"
     CDS             23967..25466
                     /locus_tag="MJE33_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HBY9647536.1"
                     /translation="MLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMALNSFIT
                     KEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSSKYHDY
                     KLELVDVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPVIIVGD
                     IDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKVPVIGV
                     VPYTDIKIEDEDSVTTRFKKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVSIRYVD
                     YGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQMLGKK
                     LKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGKKVKGY
                     EIHMGITKRGNDVRSLDLITKKLDKEVNYTEGSVNKEGNVIGTYLHGIFDEISFTRAI
                     LNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            25505..26881
                     /locus_tag="MJE33_001597"
     CDS             25505..26881
                     /locus_tag="MJE33_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HBY9647537.1"
                     /translation="MKKILIAGTNSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYIVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYKELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDASVPNADYIYIGGGFPEIFGKELEANESMRTSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTLLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLKKGNE"
     gene            26882..27853
                     /locus_tag="MJE33_001598"
     CDS             26882..27853
                     /locus_tag="MJE33_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBY9647538.1"
                     /translation="MSILSIYIGYVTDLIVGDPYSFPHPVRFIGKLINFTQCIIRKVS
                     KNDRQLKFGGFILWLITVGITYLTTYAIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLNYNYRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYASSFVSMLVFTLIYVLCRMILINLII"
     gene            27885..28955
                     /locus_tag="MJE33_001599"
     CDS             27885..28955
                     /locus_tag="MJE33_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBY9647539.1"
                     /translation="MKDLGHGANVDEMARLYGKNPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTNLRENISKYIDINPDFIIPGNGATEVIYLLMKSIKKKLAIINP
                     TFSEYRRSAELNNLDIIDLELDLENNFKLNIDIIKENIKRFDSLFICNPNNPSGNVQD
                     LKELVHLLDKHNKVLIIDETFMEFVEDESKYSLVKYIESNKNIFIIKAVTKFFGMPGL
                     RLGYGLTSNTEIMNKIYEHKEPWTINSFADILSNFIFEDKEYIKNSKEYYIEERKYML
                     QELRNIRNIKVYDTDANFILIRIYKKTTKELKKDLFKQGNILVRDASNFIGLDDSFIR
                     VAIKSHEDNKILIENMKNLLGD"
     gene            28957..29844
                     /locus_tag="MJE33_001600"
     CDS             28957..29844
                     /locus_tag="MJE33_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBY9647540.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSKVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKYTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVVILEPN
                     KVLDTMKIRLRQDYNKLKVENKEVIKKSFALLEEGLKKNNLSLVGEACTLSSLANENI
                     EKKECLNEIIKISKKYGAYGVNIAHSGTVVGILIDKSMNDKKMIDALCESNINSVYNK
                     IYTQNIINGGIKGEIEWNT"
     gene            29832..30464
                     /locus_tag="MJE33_001601"
     CDS             29832..30464
                     /locus_tag="MJE33_001601"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003724727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HBY9647541.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVDSIIDALKNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETVKLAKEKGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAQESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            30473..31699
                     /locus_tag="MJE33_001602"
     CDS             30473..31699
                     /locus_tag="MJE33_001602"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HBY9647542.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLLLKVDNINISDTFVECSIKKDGGDDIDATHTMDIYARAEIVAKNDKNKGY
                     LTLKDIDSLSTNSECKSELYKFIRVYGGTGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRKLIGDNFESILGNDKVLKITIFAPQGETVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDEGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNLDIKYVVRVSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKVSAGIFNTHSKVADARSEILVANLALMGARYEFLN
                     KINQCVTTEEAVELINNSEYREVYNILSNKCRERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            31696..32304
                     /locus_tag="MJE33_001603"
     CDS             31696..32304
                     /locus_tag="MJE33_001603"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HBY9647543.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYLSKNIDNKMLNMVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKVPDFDYILSHKKVCMVTDSKIGPKQISREIIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRIDNFDMNVVVILDEE"
     gene            32294..32869
                     /locus_tag="MJE33_001604"
     CDS             32294..32869
                     /locus_tag="MJE33_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HBY9647544.1"
                     /translation="MRNSEFITGKVPITKEEVRAISISKLNLVNAKNLIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNIKKFNLKNIEVIKGCAPINLGLNVKVESI
                     FLGGTGNNLEEIIEWSKKNLVTGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            32862..33614
                     /locus_tag="MJE33_001605"
     CDS             32862..33614
                     /locus_tag="MJE33_001605"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HBY9647545.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSNADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSKLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            33598..34719
                     /gene="cbiG"
                     /locus_tag="MJE33_001606"
     CDS             33598..34719
                     /gene="cbiG"
                     /locus_tag="MJE33_001606"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HBY9647546.1"
                     /translation="MNIEGNIAIICITENGKKLALKINSLVNNCVIYQVKNKKSNLVV
                     EETCVHTVQKKLSDFVGEIFSKFDYIVFIMATGIVVRSIAPYIISKFSDPAILVMDEK
                     GKNVISLLSGHIGGANSLTQYISSLIDANPVITTATDINQKASLDMIAKKLNAHIDNF
                     RESVKEVNSMLVNNKCVGLYVDEGYQVDTRGFKLIESKDELRDSFSNTYIEKLVVVSN
                     KINCIEDIENLNLDNKFEHCYSTQKIIKVIPRDIVIGIGCRRNTDSELMYNSLEEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKILKVPFKTFSVDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPVAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            34716..35441
                     /gene="cobJ"
                     /locus_tag="MJE33_001607"
     CDS             34716..35441
                     /gene="cobJ"
                     /locus_tag="MJE33_001607"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011701973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HBY9647547.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     GKEIIQNGMRQEVDRCKQAVEEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKGGDTPVGVVKDVGREKEEKFVCTFENMDFEKVDMTTM
                     VIIGNKSTYINGELMITPRGYTV"
     gene            35438..36190
                     /gene="cobK"
                     /locus_tag="MJE33_001608"
     CDS             35438..36190
                     /gene="cobK"
                     /locus_tag="MJE33_001608"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HBY9647548.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRELAQQHAE
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKTAITCAKLLNIDYIRFERKSL
                     IEEIDYDNKFIVDTIEEACEIANKIGTNIFIGTGSKNLNRFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIVAMKGPFSQYINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYENVFNDINKIKDILV"
     gene            36209..37687
                     /locus_tag="MJE33_001609"
     CDS             36209..37687
                     /locus_tag="MJE33_001609"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HBY9647549.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDICEDKIKNSLKSHDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIEDYQEAVEAIKHQIPHMESNEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGINAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEENGFNVRVHLKGLGENSYIQDKFVRHAVKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNKDNKIK"
     gene            37717..38622
                     /gene="hemC"
                     /locus_tag="MJE33_001610"
     CDS             37717..38622
                     /gene="hemC"
                     /locus_tag="MJE33_001610"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HBY9647550.1"
                     /translation="MNIVVGTRGSNLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLDGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPHGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSTIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPIAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            38612..40111
                     /gene="cobA"
                     /locus_tag="MJE33_001611"
     CDS             38612..40111
                     /gene="cobA"
                     /locus_tag="MJE33_001611"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBY9647551.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSSYLR
                     EAKPDCELIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEGIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLEKISENLMKEGKDKDTPVALISWATRYNQRVVTSTLENVYET
                     AIKEEVKPPTLIVVGSVVGLREKLNFFESKPLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKVEKIQNNINLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELAPILNDDDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEEKKGEILDILNSNDIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTETINSLGIKLYKESATSTIDSLVDLIIKN"
     gene            40128..41093
                     /gene="hemB"
                     /locus_tag="MJE33_001612"
     CDS             40128..41093
                     /gene="hemB"
                     /locus_tag="MJE33_001612"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HBY9647552.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     KSLPDVYHLSIDMLEDEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINSDINVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGADMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKK"
     gene            41246..41824
                     /locus_tag="MJE33_001613"
     CDS             41246..41824
                     /locus_tag="MJE33_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HBY9647553.1"
                     /translation="MLYPINLKLDELDVVIIGGGEVAYRKCKNFLEFNKNVTIVSKQI
                     LNKFYDLKGNIKIIKDDYKEIYIKDASVIIAATNNRELNMEIGLYCRAKNKLVNVVDN
                     IEISNFTVPSYIKRGDLLISVSTGGKSPSLSSKIKKEIEERYTEDYEEYLNVLGDIRK
                     KVIKKYEDKSKRKDVLNMLVTLDLEELKKFDI"
BASE COUNT        16572 a         4411 c         7464 g        13425 t
ORIGIN      
        1 aaaaatagag aaaatattca aatatatatt gactattcag aaaaatatga ctaatatata
       61 atttatagag aaactaattt tatatataat gactacttag ataaaagaat ctaaaaaaaa
      121 ttatgagagg aggagtaggg tataaaatca gattataagt tttaagttat tatactaaaa
      181 agctataaaa cagttatcat gtattttatg ttaaaggaaa ttgtaggtag tttatcgaat
      241 tgtttaatca gaagaaaaaa tttcacatgg ggaggtaact gagatatgtc agaaaaagta
      301 aatgttaaaa cagtcatcag tttcgcaggt gcgtatgtag caacagtaat cggttctgga
      361 tttgcaacag gacaggaaat aatgcaattc ttctcttttt atggttttgc aggtattatt
      421 ggtgggataa tttcaatggt attattctca tggatgggtg cttcagttat gtcaaaaggt
      481 aaagagcttc aacttaaaga acctatcaaa atttatcgtg tttattgtgg taagtattta
      541 ggaatattct ttgaatggtt tggaccttta ttcttatttg gtgttttcgt agtaatgatt
      601 tcaggagcag gagcaacttt aactgaatac tatggattaa atccatttgt aggtagagta
      661 ggtatggcag tagtagcatt attaactgtt tcactaggtc ttgataaatt atcaaagata
      721 ctaggtggaa tagggcctat aataataatc tttacacttt tagttggtgg tataagttta
      781 gctaaaaaca taggtaatat aggagaagct gcacaagttc ttagcactgt aaagatagct
      841 aaaccagttc caaatgccta tctatctggt gtaatatata ctacttataa cacaattgtt
      901 gttatggcat tcttaactgg attaggagca agtgctgcta acaaaaaaga agctatttgt
      961 ggtggtatat taggtggagt agcacttatg gctgcagcta taatgatgca cttagctatt
     1021 ctttctgata taggaaactt atattcaaaa gcaataccat cattattctt agcagataag
     1081 atttcaccag taattggagt tttattctca ataattctta tattaggtat atatacaaca
     1141 gctgtacctt tattatggtc agtaactaat agatttgtag atgataaaca tcctaagttt
     1201 aaattaataa ctttaatagc agctatatta ggtcttatag gtggcttcct accatttgat
     1261 aagctggttg gtattttata tccatatact ggatatatgg gcgtcataat actggtttgt
     1321 gttctttata gacagattac aaaaacatct ggatatcaag aaggaatcag tgacaaataa
     1381 gtatttttta atattgtaaa ctcaagagct atatgaaaac aatgaaaatg ttttcatata
     1441 gctcttttta ttttttagct cgtttattat gaaatagaaa aaacatgttt tttagttaca
     1501 ttacatatat ttataattat gtataaatat atactaatca ataatttaga gtctaagata
     1561 agtttataaa tcatatattt agaatatatg ggaggagtga taagattgaa tgtaaagttt
     1621 aatattaaag gtataattta tggagttata gctcttgtat tcgttatagg gggatttata
     1681 ttcataccaa gtatgtttgt agagaaatct aaacccatag attatacagt attacaaaga
     1741 aattcaatac cagaaaagat attagatgtg atggataagt atacaaatga agaaagagca
     1801 ttagcggcta aaatcgataa caaaatatac attatagtga ctagaggaaa taataaatat
     1861 ggtatagaaa tggacaaaat agaaagtgtg gttgaggaag gaaaggatgt tttgagagtc
     1921 aagataaagt ataaagataa ggaagattct tttccataca tagttgttga aacaaatatg
     1981 agtgaacttc cagatagaat agaattaaat gcgacgaaat aagaaggtat tttataatta
     2041 aataactgca tattgtacaa taaagtcatg gtttttatta atgtaagtaa attgtgtatg
     2101 gttcttattg ttgtaaaagc catgacttaa aatgcttata aagtaagata ttaacagtag
     2161 tatttattac tacattaaat ggggtctgga aatacttaat attctaacta tttggagtga
     2221 taattctagt ttcagtaact atgtagtcta attgtgcatc atgagtttct tttggaatag
     2281 aatcgaatat ctggaagtca aatgctatgc cgatagttat agcatctttt cttaagtttt
     2341 ctaaaaatct atcataaaat cctccaccat atcctaatct atagcaatgt atatcatatg
     2401 ctacagctgg tacaattaca acatctaaag ccttaatatc aattgtaggt gatttttcgt
     2461 taggttctct tattccatat gctccaattc tcaatccctc ttttaaatct gttatttgag
     2521 tagggattaa tgtatgattt tcttttattg tgatgggact tgatactatt ttctttagag
     2581 acaaaagttt tttgattaaa ttatctgttt gaacttcatt gttaaaattt agataaagca
     2641 ttatattagt tgcatttctt atatcttcca tttgaagtaa tttttcagtt attaaatttg
     2701 aattactaag tacaaagttt gcactcttat tttttcttga ttcaataact ttttttctaa
     2761 attctttttt cataaaataa ttcctccctt aatatgttat aatgtgtaca gcaattgtat
     2821 ttaatataaa ttttagtata atttattgta ataaagcaat atatttgggt aaatgtaata
     2881 aaaataacag ctagtcatat aataataata gtttatgtaa aaggagatgt atcttgtgat
     2941 ttcaaagaag aaagctattt ttttaggggt gattttagta attataacag ctatggttac
     3001 atcagcattt cagttgacat tggggaataa agttgtaata tcgaaagaac tctatgaaga
     3061 ctataaaaaa tatgataagt tgttaggact agaatctatt ataaaacaag atttttataa
     3121 aaaagtatca gatactgatt tggtaaatgg agcagcaaaa ggtctatttc taggaacaaa
     3181 tgataagtat tctggttatt atactaaaga tgaaatggag aatctaatta atgatagtga
     3241 aggttcttat gttggtgtag gtatgtatat aggggcatca aaagatggtg gcttagtagt
     3301 agttcccatg aaagattctc cagcagagaa agcaggtgta aaatcagggg ataaattagt
     3361 aaaagtcaat ggaaaatctg tctcatataa aaattcagat gaagctgttc gtatgatgaa
     3421 gggtaaaaaa ggtaaaacag ttgaactgac tatattaaga gaagataaac aattgaactt
     3481 taaagtaaaa acagaccaga taattgagaa gagtatagaa agtaaagtaa ttgataatga
     3541 tttaggatat atagaaatta cccaatttat atcaagtaca tatactgact ttgataaagc
     3601 tttaaaagaa ttaaaagcaa aaaatatcaa gggattagta atagacctta gaaataatcc
     3661 tggtggaatg ctagatatat gtaaagaagt tgctgatgag ttaattggtg aaggaaccat
     3721 agtatataca aaagataata agggaaatac tgagtacttg aaatctgata aagaaaaatt
     3781 gggacttcca atagtagtgt taactaatgg agaaagtgct tctgctgctg aaatattaac
     3841 agctgcaatc gttgataata aagaaggtat ttctgtaggg actacaactt ttggtaaagg
     3901 gttggtacaa tcagttgtaa gattgaagga tggaacagga tacaagttaa ctactgctca
     3961 atattttaca cctaatggag attatatcaa tgaaaaaggt ataaagccta ctattgaaga
     4021 aaaagatgaa aataaacagt tagatgtagc aactaagtgg cttagagaac agattgataa
     4081 ataggattta atttttataa acttatacta taaaataaag aacattataa aagagatgtc
     4141 tcaaaatgaa ctttttagtt catgaggcac tctttttgta tgaaatcaaa tgtattttca
     4201 tcattttaac aatgaaaaag aggcttatct ctatttttag gaagcctcta taactttata
     4261 ttgaatctat ttattaactt tgtacataca gataccattt atataagttt cgtgtatatt
     4321 aaagtcatta tctgttacta taaagtctgc atccatacct tgatatatag agccacagac
     4381 attatcaata ttacaactta aagcagggat atatgtagcc atttttattg catcttctaa
     4441 agttgatatg ttccaatcaa ctatatttct cacagccttg tccatagtta atacacttcc
     4501 agctaaactc ccactattta atcttgcaac actatttttt acatttacat taaacttacc
     4561 aagagtatat tttccatcct ccatagcacc agctctcata caatctgtaa ttaaagctat
     4621 gttattacta gacttagcat ccatcattat tttaacagct attgggctta catgatgacc
     4681 atcacaaatt aattcagcat aagtatcttt tagtaccata gcagccccaa ccattccagg
     4741 ttctctatga tttaaaggac tcatcccatt atatgtatgt acaaacactt tagcacctga
     4801 attaacgact tgtttaactt gctcaaatgt agctgaacta tgaccaagag aaactgatat
     4861 attattttta gttgcatatc ttgttaattc tattgaacct ttacgttctg gagcaattgc
     4921 tattttcctt attaaatttt tcgatttttt ttgccataat tttaatatat tgatgtcagg
     4981 gtctttaaaa tatttttcat tttgagcgcc cttatatttt tctgtaaaaa atggaccttc
     5041 taaataaatc ccttgtattt ttgcaccctc aactcgattg aagctattgc ttatattttc
     5101 tacagaatca cttagtgtat ctacagaatc agttaaagtt gtaggtagga aagatgttac
     5161 tccatatttt aataagcctt ttgaaattgt gtttattgct tcaaatgtat tgtccatgac
     5221 atctgcacct aaaagaccgt gaatgtgagt atcaactaag ccaggagcaa tatatttacc
     5281 tgaaaaatct ataacattat cagtattatt aggtctttct tgttgaaatt caccgaattt
     5341 gccattaatt atactaaggt aacctttttc tttgattcca tgtttcataa aaaatttatc
     5401 tgcataaata aaacttttca aaataacctc cttataatta tactatcatt gtggcattga
     5461 cgcttataat atcattctgt tttcttgaaa gctatttaac ttactatctt acatgtctta
     5521 ctctatatac aaatttatcg cttctagcga cactcaaagt aaattcaata actatattgt
     5581 catcattata tgtggtacga agtaatttca gacaaggtga atctcctggt acttccaata
     5641 acttagcttc cttatcagag attattccag ctgaaaattc ttcatcagca actttaataa
     5701 tctcgccata atcttctcta aatatttcat ataaaggttt ttgttcgatt ttttcttttg
     5761 tcaatcctat aaattctttc ataggaagat atgttctttc aagcatcata ggaaggtcat
     5821 cagcaagtct taaacgcatt atttttatca ttttttcacc agtagttata cctaattttt
     5881 cagagaaata ctttgtactt tctactacct caaatgataa tacctttgta cctggctttt
     5941 ttccttgtgt tctcatataa tctgtaaaac tataactttc cattaagttt tgagaatttt
     6001 gacttagtgc agcaacaaaa gttccttttc catgtctttt gtatatatat cccatatgtt
     6061 ccaactcagc catagctagt ctaactgtgg ttctacttac tccatattca tcacaaattt
     6121 ttctttcgga aagcattcta tcgtttggtt ccatctcatt ttcaatctta gttcttaata
     6181 tctcaacaag ctggtcatat attggtcttt tatcatttct ttttatcata aaaatatagt
     6241 cctttctaag tggttattac cacaatttga ttatagaata tagaattatt tatgtcaatg
     6301 aataaagttt aatcaaaatt caataagtaa ggctattaat ttattataaa attatggtac
     6361 attgacaaaa caagtaaaat aatttatatt caaattggta ataaccactt tggatatatt
     6421 atatgaggta ttttttattt tacagattaa tttattgttt agaaaagtaa aaattaataa
     6481 tgaggagaat ctagtatgat tacagtaata acattcaacc catctataga tagaatgtat
     6541 agagtgaata atataaacat aggtgaggtg caaagggtag taagtaagaa tgcaactgct
     6601 ggaggtaaag gattaaacgt agctaaggta tgtaaaattt taaaagaaaa tcctttagct
     6661 atgggatttc taggcggatt taatggagaa tttataaaaa atgaacttag aaaactagat
     6721 atacaaaata aatttacaaa aatagagcag gaaactagaa attgcttaaa cataattgag
     6781 gacaataaag ttagtacaga atttttagaa aaaggaccaa tagtagaaaa aagcgattta
     6841 gagagatttg aaaatgatat agaggaagta ctaaaaaata ctaaaatact ggtagcttct
     6901 ggtagttatt gtgagaatat gccgatagac tattatgaaa agataggaaa tacatgtaga
     6961 gcaaataata taaagtttat attagataca tcaggagaag cattaaaaat agctttaaaa
     7021 tcaaaaccat atttaataaa accaaatata gatgaaataa gacatttatt aggaatgaat
     7081 atagaatcaa gagaggaaat tatcttgtca ggaaaaaaac ttattgaaat gggtgcagag
     7141 aatgtatgta tatctttagg aaaagatggt atggtgtatt taaacgaaga taatgtatat
     7201 gatgtaaaag taccagttgt aaagtgtata aatactgtag gtagtggaga tagcacagtg
     7261 gctggattta gtgtaggtat tttaaggggt tataaaatag aaaatttact taaatttgca
     7321 aatgcttgtg gtataagtaa tgctttaaat atggaaactg gatttgtaga tttgggagaa
     7381 attgataaat atcaagattt agttgaagta aaaaagttaa gttaaataca tttaatggga
     7441 atataataaa tttaatgttg ttgtaataga ttttaatttt aatataaaag atatagtctt
     7501 atataaaaat aggttagaaa aattcatttt taacctattt ttataataaa tatctatttt
     7561 actgaattta taattttctc tagtaatctt tgagctttga tacactcttc tccattaata
     7621 gctggtttta tattattttc tatacaatca ataaaatgat ttgatatatc ctcaaaaccc
     7681 ttttgtttta atatatttac ccatgcacca ctttgcttta aagttaggtt tccaccttct
     7741 tctatttcca gtacattaag attcttaact ctttgaatct taccctttga aagaatttca
     7801 acttgttcta actttgttcc agaatctcta tgcatagagg tgtttataga gaaattttta
     7861 cctttaagtt tatgacttac aaatataaga ttcttattgt ctgttaaaaa taaatcctct
     7921 ccactaattt caacatcttc atttgctaac cataaagcag tgtcaataac atgtatatag
     7981 tcatctataa gagtactgtc gaatctaaca tttcttaagg aatttaatcc atgtttacat
     8041 atattgatag atacaatttc tgtagcatta tttttaattt ctttgtacat aggacagaat
     8101 ctacggttga atccaaccat taaatttaag ttttttttcg tacttaactc gataagttct
     8161 tctccttgac ttactgttga agcaagaggt ttgtctacat atacatgaac acctaaattt
     8221 aataatattt ttataatttc gtagtgtgtt tctgtagagc tatgtagaaa aatacaatca
     8281 cattttttag ctagggattc aatagaatcg aatggcatga ttctataatc actacagact
     8341 ttttcccttt taactttatt tggagtaaaa gctcctacaa actcaaatct ctcagactta
     8401 gttaatatag gtaaatatgc cttttgagct atggagccaa gacctatcat tcccattttt
     8461 atatttttca taatactttc tcctaaggtt tttatattta cagaattgat tataattcag
     8521 acacataaat ttgtaaactt tgtatcaaaa aataaattta aagcttatat aagtaaatta
     8581 gcgaaataag aaatattgat aaagtaattt tgtaggatat ccataaaatt gattgatata
     8641 aatattttat gtattgacaa aaaattcaaa ttaatttata tttggattag tggtaattac
     8701 cactttggtc tgaacaagct tttatcgtaa aataaaaata ctaaagtata tagagtatga
     8761 ttttattaaa tttttaaaga taaactataa aattaattaa aatctatagt gtatatttta
     8821 tagtgagttt aagtagtact aaaaaatcta agcaaaaaat tataaaagag tatatttttt
     8881 tatagtttaa agtggttata acctcattac caaatgtaaa cgttacaagt atgaaaggag
     8941 aatgaaaatg tttaaattag aagaacaaaa actaaaagat ttaggtgcaa taataacaac
     9001 taatgaaata aaacaacagc ctgaattatg gttagaaact tatgaaatat ataaatctaa
     9061 caaagaaaaa ttaagtagat tcatagatac aataagtaat aatcatgggc agtttagagt
     9121 tatatttaca ggtgctggaa cttcagctta cataggaaat tcaatacttc cttaccttaa
     9181 aaataaaaat gacataagaa aatacatatt tgaagcaata cctactacgg atatagtttc
     9241 aaacccatat gattacttaa aaaaagatat accaacatta ttaatttcat ttgcaagaag
     9301 tggaaatagt cctgaaagtt tagcagcttt aaatttaggg aataagattg tagataattt
     9361 ttatcattta gctataactt gtaatccaga aggagaactg gctaagatga ctaaaaacga
     9421 cgaaaataat tacttgttat taatgccatc aaaatcaaat gatgaaggct ttgccatgac
     9481 aggaagtttt tcgtgtatga tgttatctgc tatgttaata ttcgatagtt tagaagatga
     9541 tgtagaaaag tcatatataa atgcaataat tgaaatggga agaaatgtta tagatagaaa
     9601 agatgaaata catgagttaa taaataaaga ttttgataga gttgtatatt taggttcagg
     9661 aggattaggt ggtttgacac aagaagcaca actaaaactt ttagaactta cagcaggaaa
     9721 aatatctaca gtgtatgatt ctcctatggg gtttagacac ggaccaaaat catttattga
     9781 tgaaaataca cttgtatttg aatttgtatc taactgttta tatacaagaa aatatgattt
     9841 agatgtttta gaggagataa aaagggataa aattgctaaa ttcacttgtg cagtgtcagt
     9901 agaaaatgaa aataacttct caggaacaaa gtttgagttt aaagaaaagt ataataaatt
     9961 accagatgtg tatcttgcaa tgccatacat attgtttgca caaactatag cattgtttgt
    10021 atcagtaaag gttggaaata agccagatac tccatcagct acaggaacag taaatcgagt
    10081 agtaaaaggt gtaacaatat atgaatatta aaagataaga ttaaaatatt ataactaaat
    10141 aatttgggag ggtatcaatg tggcattagt aacaacaaca tcaatgctta aaaaggcaca
    10201 agatgaaggg tatgcagtag gagcatttaa tgtagaaaat atggaaatgg taattgctgt
    10261 tatagaagct gctgaggagt taaattctcc agtaatatta caaactactc catctactgt
    10321 aaaatatgca ggtcttgatt attatctagc aaatgcaaag gttgctgcag aaagagtaaa
    10381 tatacctgta gcaatgcatc tggaccatgg ttcaagtttt gaccttgcaa tgaaagcttt
    10441 aagaagtgga tatacatcaa taatgataga tggttctcat gaaagctttg aaaataatat
    10501 atctatatct aaatcagtag taaaagcatg tagtccttct cttataccag tggaagcaga
    10561 acttgggaaa gttggtggaa aagaagatga tttagatggc gggaatagta atgattatac
    10621 aaatccaaaa gaggcaaaag agtttgtcga tagaacagga atatcttcat tggctattgc
    10681 tataggtact gcacatggat tgtataaggg tgaacctaaa atagatttag ataggttatc
    10741 agaaattaaa aatgtagttt caataccttt agtattgcat ggaggttcag gaatacatga
    10801 tgatattata aaagaagcta taaaaagagg tattgcaaaa gttaactatg caactgaatt
    10861 aagaattgca tatagtaaag gggttaaaaa agttttagat gaagattctg aggttataga
    10921 ccctaaaaag tatggattag cagggcttca atcagtaaag gattttgtga aagaaaagat
    10981 taaagtgtgt gggtctgtaa atagagtata aaaataaaat atcctaaatt tattttagat
    11041 aagtttagga tatttattat atgaaaaatt tatattaatt atatgtaaat ggtgtatttt
    11101 tttcatttaa ttgatgaaaa atatcagctt ttattggtaa agtattacat atataattaa
    11161 aatatactaa aaattgaact atatatttta ttattataaa atatttatat taaattgtgg
    11221 aattaaaata tactaggata taaaattctt aaataaaaag aaaaggttgt ttttataaaa
    11281 aatatttttt ataccacttt aaaaaacaca ataataacac tgagggaggt ggggatatga
    11341 gagaacgcat tattcttgaa acattaaaaa atagtagtgg ttatctaaac atagattatt
    11401 ttgcagataa gttgggggta tctactcgaa ctatcagaaa tgaaataaaa aagatagaaa
    11461 ctataaaaga atgtaatggt tttaaattag aatataagtc taaattagga tatattctaa
    11521 atattaaaga ccaagataaa ttcgagcatt atctaagaaa cttaccctca gatttagttg
    11581 aaaatccaga acaacggttg gagtctataa tagtagagct tttagtaaat gaaggttata
    11641 aaactataga acagttaagt aaaaaatttc tagtaagttc atcccaaata aaaaatgatt
    11701 taaaaaagat agatgaaaag ataaaggata cagaattaaa actagagcga aaggctcatt
    11761 atggaattaa aatagaaggt tctataaaag aaattcaaag tatattggtg gatagttatt
    11821 ttagagggaa tagaaatata acagagtaca ggaataagtt tatagataat ttaaaattag
    11881 ctaatattag aagtacaata aaaaatgtac ttaatgaaca tgatttagaa gcaaatttaa
    11941 cagaacttga ggaaatatta gcacaaatta ttatattgta tataagagtt agtatgagag
    12001 tagtgggaaa tgctagcgat ttaaaattat atacagaaga tttaattata gataaattat
    12061 tagataaaat ttttagagat aaaaaatata accttaatta tgatgaaaag tattatttaa
    12121 aggagcttat aaaactaaag actaaagata agaaagccac tataaagaat attgataaga
    12181 ataaacttca agatattatg ttcgaatttt ttagagagat agataaaaaa tataactcta
    12241 attttttaga agataaagag ttttttaact tattttactt acatattgca tgtttaatag
    12301 aacgtataaa gaagaaccac aagataataa atcctttttc agttaaaata agtcaacagt
    12361 atccaactgt ttttaattta tctattcaat tttcaaaaat catagaaaat gagtatcata
    12421 taaaaataag tcaagatgaa atagggttta tagctactca cattgctgtt ccttttgaaa
    12481 agagagaaga agctaatttt aataaaaaat ataaaatagc aataatatgc tcttctggtg
    12541 gaggtagtgc atttttgata aatttaagac ttaaagaaat ttttccaaat gctgagataa
    12601 gaaatttttc attactagaa gaaaaagctg taatagaatt tgcaccagat ttgatttttt
    12661 caataacaaa tcttttattt gaaataaatg ctccagttat acttataaat gaaattttag
    12721 acgaattgga ttatttagat attaaagaaa gtgttagatt tgcagaccat ataggaaata
    12781 tatcaagccc aaaacaatat atattaggtc tatttgataa gaatcacttt aggtgtataa
    12841 aagataaagc taagtataag gatatccttg atagtatgtc aaaatgtata gttgacgagg
    12901 gagcttgttc tccaacctat cccaaagatg tatgggaaag agaaagttat ttgagtacta
    12961 tttatacaaa tggggtggct atacctcatc caatcgagat gacaggaaac aaaaatataa
    13021 tatcagtagc tttgatacaa actgatatta tttatgaaaa tagggtgcct aaaattattt
    13081 tcatgatttc attaataaag ggaaatttag aacttcataa gcaaatatct aaatatttaa
    13141 gtaagattat ggcaaataag gatatggtag acatgctaaa taagtcacaa tcttatgaag
    13201 agtttatgta taagttaaaa atctatctgg gaggttagac atgaatatag agttttttaa
    13261 gaggttgtca gaagcagatg gtatagcatc taatgaagaa gaggttagac atgttttatt
    13321 ggaagaacta aaagcttata gtgataaaat tatctgtgat gggctaggga gtatcatctt
    13381 ttctaaaata aaagatgaga gtgcaccaaa cgtaatgatt tgtgctcata tggatgaagt
    13441 tggatttatg gtgagaagta tagacaaact tggtatgatt cacttaataa caataggagg
    13501 agtaaaacct cttgcacagt ttgtacaaaa agttagaatt acaacaaaag aagggaagaa
    13561 aatccccgct gtcataaatg ctacatataa taatggtaag gctgaaaata tatatgcaga
    13621 tattggagca tatacagaag aagatgtata taacctaggg attaatgttg gggatatggt
    13681 aacatataca acaagctttg aggaatttac tttaccagac cgattagtgg gaaaggcttt
    13741 tgatgatagg ataggatgtt ttgtgatggg agaagtttta aaagaactta ggaaagaaaa
    13801 tctaaattgt aatattcact ttgcggctac aagtagtgaa gaggttggaa taaggggagc
    13861 aaagacatca acacagttaa taaatccaga tatagtattt gtaatagatg tagcatgtgc
    13921 taaaaatgag tttgtgagag actatacaaa taagaaacaa attgataaag gtgtaatgct
    13981 aatgcataga gataggactc tagtccctag tagaaaaatg atagattatg taatggaaat
    14041 tgctgataaa aataatatac cacttcaaca tgatatgttt gaatcaggtg ggactgatgg
    14101 aggagaagca catctcgtaa atgaagggaa accttgtgta gtaacttgtg taccagttag
    14161 atatggtcat tgtgcatttt caatagtaag taataaagat ttggagaaca ttataagatt
    14221 gtatacacag ttaattttaa attttgatga agaacaatat aaacatataa gaaattttat
    14281 ctaaaggaga ataagaaaat gtcaacagaa gtacaaataa tggaacttat atcaactgca
    14341 ggagagagta aatcgaaagc ttttgaggct ttaaaaaaag tgaagtcaaa agattttaaa
    14401 ggagcaaaat tgttaataga ggaggctcgt aaaatagata ttgaagctca caggatacaa
    14461 acagaattaa taactcaaga tttaaatgaa gatgttgaaa gtccagcaat gagtctttta
    14521 atggtacatg ctcaagacca ctatatgact tctcagctag caaaagactt gatagaggct
    14581 ttgatatatt gtcttgataa gtaattgtaa tatagaaata gattatttat gtaaaaattc
    14641 ttttaaaagt ttagatgctt ttaagagata ataaaaatac aaactttagg aggaattatt
    14701 atgaaaatat tattatgttg tgcaggtgga ttttctacta atatgttaat gcaaaatatg
    14761 aaaaaggtgg taaaagagag tgctaaactc aatgaaaaag attttgagtt tacagctata
    14821 ccagcagatt caatagaaag tgaagttgat gattgggata tagtacttat aggtcctcaa
    14881 atagctcaca aaatagactt cttaaaagga atattagagc caaaaaataa gccttttgca
    14941 gtaatagata aagatgtata tggaaaaatg gatggagcta cagttatgaa gttggcatta
    15001 ataacttata agaaacatca attagctcaa ggataataaa aataatgagg gaaaagggag
    15061 attagatatg ttttcaaaat ttgaagcatt tatgaataga gtatttatgc ctttagctca
    15121 taaggttgac aatcaacgtc accttggttc tataaaggct ggtatggttg ctatgacacc
    15181 attcacaata ttaggaagta tatttgcaat acttccagca cttccaaata tgcttggaga
    15241 aaataatcca ataagcaaat tcatacttag caattcagca atattggatt taccagtaag
    15301 tctttccata ggtttaatag gattatatgc ttgtatgtgt attgctttta atctggggaa
    15361 ctactataaa ttatatatac caggttgtgt tgcactatca actttttctt ttttattcct
    15421 agtagcctca tttacaccag atggagcttt agaaatagga aatttaggag ctaaagggct
    15481 atttacaggt atgatagcag ggcttggttc tgtggagtta tataattttt gtaataagaa
    15541 gaatatcaca ataaaaatgc cagaaaatgt acctgatttt gtatctaggt cttttgaatt
    15601 aatacctact actataatag tttgtggaac ttttatagtt gctcgtttta tttcattaaa
    15661 agtatttgga gaattacctc cacagatact tactcaattt ttatctccat tagtaggaag
    15721 tatggataac ccatggtcag ttcttgcact taactttgct atatgtacta tattcttttt
    15781 tggaatacat tcatcagtat ttagtcctat aactagacct ataatgatta catttatagc
    15841 tgaaaacata gcagctatgc aggctggaga aacaatacct catttcttta cagcaggagc
    15901 ttcaagtgca ttctttggat ttacaggatg tggaattagt ataggttgtg taattgcatg
    15961 tatgttatca aaaaatgctt cctataagaa gattggacgt gtatcattat tccctgcatt
    16021 atttgggata aatgaaccaa tattatttgg agcaccgata atattaaatc caataatgtt
    16081 tataccacat gtatttggtg gagctataat aggtacacta ccaatgtttt ttatgcactg
    16141 gggatggtta gcaaagccag tatttgaccc tccatatgta ggggtattct tagaaggatt
    16201 tttaactaat ggagattggc attctatatt agcaaatgca ttgcaattag tattagcagt
    16261 tttattatat tggccattct ttaaaataat ggaacgtcaa caaaataata aaaaacaaaa
    16321 agaagaaact aagaatatat tctctaaaga agaagaagac ttattggatg atttaggatt
    16381 ggatttctaa tattttgtta tgaataatac aatggagaga ttagtatcct taatctataa
    16441 aagaggtgtt gacgctcttt tgattaaagc aaaaaatact aaaagatata taggagcttt
    16501 atcaggtagt ggtgtatatg tgcttgttac taaggataaa aattatcaaa ttctagatgg
    16561 cagatatatt gatgaagcag ataaaaaaac gactggtttc ataaaaagga ttgtatctca
    16621 aggttcttat ataccaacta taattgagtt attaaaagaa ttaaatataa aaaagttagc
    16681 aatagaaaac caagctatga gtatacaaga gtatctttca ctaaaaaatg aaggttttga
    16741 aatcaattta ataacaaatg aattatggaa gctaagggct ataaaatcta aagaagaaat
    16801 taaacttatt aaaaaggctt gtgagattac agatgaagta tttgatgagg ttatttctga
    16861 aattaaaaaa gatatgactg agctagaagt atcagctcta attcagtatc atgcattaaa
    16921 aaaaggtgca agtggaatgt cttttgaaac aatagtggta tcaggagaaa gaggtgcaat
    16981 gccacatgga agacctacaa acaaaaaatt gaaagtgaat gaagctataa caatagattt
    17041 tggtgttgtt tatcaagggt atcaatcaga catgactcgt acaatatcta taggcaaacc
    17101 tcctaaaatt attaaagaga tatatgatgt agtattggaa gcacaattat ctgcaataga
    17161 gtcaataaag gaaggaacta gggcaagtga tgttgacaaa gtggctagaa aaattataga
    17221 taaacatgga tttggcgaat attttaatca tggactgggt catggtatag gtcttggaga
    17281 tggtgaagtt cccactttaa atccaaatag tgaagatata ttagtggaag gaatggtaat
    17341 gtcttgtgag ccaggtattt atattccaaa tgtaggggga gttagaatag aagatgatat
    17401 tgttataata gatggaaaag gtgttccact caataagaca tccaaagagt ttataatatt
    17461 aggagagtaa aattatgaaa tataatttta ataaagtatt aaatcgtaaa gaaggaaatt
    17521 gtagaaaatg gagcaaccat gtgattaaag aaaaatttgg cttaaatgaa gatgcaattc
    17581 ctatggattt agctgatatc gattttgagt gtgctcctgc tataaaagaa tctatcttaa
    17641 aaagagcatc agtaggagat tatagttata cttttatagg agatgacttc tatgatgcta
    17701 ttataaattg gaataaaaga agatttgatg tatatataga aaaggattgg ataaaattaa
    17761 cttttggtac tgtttcaacc cttcattata tagtccaagc atatactaaa gaaggagagg
    17821 gtgtattgat aaatacacct gcttatgacc cttttgcaga ggctgtagta aataataata
    17881 gaaagttgtg ttgtagctca ttaaaattag ataacaatag atattattta gatttagagg
    17941 atatggaaaa tcaaatgaaa tacgagaata taagattgct tatattctgt agtccacaaa
    18001 atccatcagg tcgtatatgg acaaaagaag aactatatca agtgtcagag ttatgtttaa
    18061 agtataatgt aattttggta agtgatgaaa ttcatagaga tataatattt aaagattata
    18121 gatttgtatc tttgtggaat gctcatccag aaatttataa aaatagtata atctgtgtat
    18181 ctcctaataa aggcttcaac ttaggtggat taaaaacatc atatgtatta ataagagatg
    18241 taaaaattag ggagacttta ttagaaaggc taaaaagtaa ttcaataact tctccaaatg
    18301 tatttgcaat accagcaata gttgcagcat ataatgaaag cgaagaatgg ctagatgcta
    18361 tgactagtta tgtagaggag aattttgaaa tagtttatga tttctttgaa actaatatac
    18421 caaaagctaa agtgatgaag tcagactctt cttttttagc ctggatagat gtaagagaag
    18481 tatttagaaa tgaagaagaa tctaaagaat tttttagaca tgcaaactta actatggtag
    18541 ttggaagtta ttttgtgaaa gatggagatg gatttattcg tattaatgtg gggtgtccta
    18601 gagaaacatt aaatgaggct ctaaatagaa tcaaaaaaac gtatataagt atgtattgtt
    18661 aaatcataaa aaaagtatct atggtttata taaaaacaca ttatttagtt atcaaatatt
    18721 actggaattt tgtattccag taatattttt tgattaagga atattattga ggaatattaa
    18781 ataatattat ttcataccct tatattaact tatataaaaa tattttatta ttttgggaac
    18841 gggttagcta acctttatct agtgtatatt tatattgtaa gaagcagtaa aaacttttcc
    18901 tttactaagt ttattaataa acttcttatc tttataaatt ttgttagaat ttatactatc
    18961 agcaagacca taccaaggtt ctatacataa aaaaggagca gtagaattag tttcactata
    19021 ataaggagtc caaataccaa ctagagggaa atcttccata gatatagtta catatttact
    19081 accatcatta ttacatatag acacttcatc tatgttggtg tatatcaaag catcattctt
    19141 gaatacttct ggatttaact gtaagttttt taattttcca ataggtatta tatcatctgt
    19201 aaaagaacca tttaaattga ttttttctac atcattttta cttttaaact caaggtagtg
    19261 tttagaaaaa tcattttgtt cgtaaaaagg aaggttgaat gcaggatgtc cacctataga
    19321 aaagaatata tctttagaat cagtattttt tacaatccat tctacctcaa tactattatc
    19381 atttaatgta tagtttatga ttagctcaaa agaatatgga tacttcttta aagtagattc
    19441 attatctgtt aatttataag ttacaaatgt atctccttta tctacaatct caaagtccat
    19501 atctctagca aacccatgtt gagtcatgct atataaattt tcttctatta ttgtttcatt
    19561 atcaaaaagt ttacctacta tagggaacaa tataggtgat tgtctacccc aatatttttt
    19621 attaccatcc caaagaatgt ttttgtcgta ttttttagaa aatattttag ttagttcagc
    19681 accaaaactg cttgattcga taattaaatt ttcattttgt aatatattca tttaaaatct
    19741 cctttattat attttatgag tatattatac tataaagtat aaagattatt aaattttatt
    19801 tgatattata tttgaaaggt agattttagt atattttgaa aattttgttt tgaaaatttg
    19861 ggattattta agaaattttg tattataaat agaaggaaat agataaattg tatagaataa
    19921 aatatattaa gataagttaa taatgttaaa tgggaagtga gtttatggaa aaaccaaata
    19981 caaaagttgt taactttcaa gagtataaag acagaaaaaa ccaaagagaa gtaaatagag
    20041 acgaactttt aaagcttata aaaaaaattg tgacttcaaa atagggagtt gttaaactcc
    20101 ctattttaat gggtttcata ataattaaga cattctaagt ccatctatat aatctttgaa
    20161 ttgtgagaaa tctgattcac tacttaattc tatggtttct gaaccttccg tataaaaatt
    20221 cttaagatag atagtataat tattatcaac acaatcatga taaatacatc tatatccatc
    20281 ttcatacaaa ctttttaact tagaaaaatc atccacaata tcatctcctt aaagttcttt
    20341 taaatatatt tttacaaaaa gcatctaatt gtatgcaaat tgacaatgta aaataaagga
    20401 aatgaacata aaatgtagaa taatttttat atgtatgtaa aaataaaaag aaaaagttga
    20461 aatttttaga aaataagagt attttttatc taaaaagaga gtttaaaaaa taaaaaaatg
    20521 ttgggaggat aaaaaatgag tttattagaa agtatatcaa aaaacatata ttccctagat
    20581 aactcttcta ttgaaaaaac aaagcagaga ttggataggc ttatacatcc aactggaagc
    20641 ttaggaaaga tagaagatat ttgcatgcaa ttagcaggaa tatttggcaa tgaaaatttt
    20701 gacacaagta aaaaggttat aattgctttt gcgggagacc atggtgtata tgaagaaggc
    20761 gtagcacctg accctcaaaa tataacaaaa ttacagtttc caaatttttc gaaaggtcta
    20821 tgcggagttg gagtaataag taaatttgta ggggcagatg ttgtagctgt agatgtagga
    20881 ataaactgtg atgaaaaact ggatggggta ttagactaca agataagaaa agggacatca
    20941 aatatggcaa aaggtccagc tatgagtaaa caagaagcaa ttagatgttt agagataggt
    21001 attgaaatag cagagcagtg tatagaaaaa gattataagg ttattggtat tggggagatg
    21061 ggtatagcaa atactactcc aagtacagca ataatatctg taatttcagg gtgtgacccg
    21121 ttagaagtta ctggtatagg tgcaggtctt aagaaagaaa gactaaagca taaggctgaa
    21181 gtaattagaa aggctattga aattaataat ccaaatccaa ctgatggagt agatatttta
    21241 tctaaagtag gagggtttga aataggctca atggcaggtg ttatattagg ttgtagtgct
    21301 aataggattc cagtagtaat tgatgggttt atttcttatg cagcagcatt aattgcatat
    21361 aagataaatc caaaaactag agaatatatg atagcatctc atttatcagc agaatcagga
    21421 actaaaagag cattagacat attaaaatta gaccctctat taaatatgga tatgagatta
    21481 ggtgagggaa gtggagctgc tttagcattt gatataatag aagcttcaaa ctatacttat
    21541 aaaaatatgg caacttttga tgaaattgac atgggaagat aaatataaaa atttaatatg
    21601 taggagtgtt ttgatgagta aaattattct agtaacagga ggagctaggt cagggaagag
    21661 taactttgca gaaaaactat gttttgacag aaataataat acagcttata tagcaacctc
    21721 tattccattt gatgatgaaa tgaaagatag agtaaaaaaa catcaagaga atagacctag
    21781 aaactggaat acttatgaaa tatatgaaga tatatattcc ataattaagg agatatataa
    21841 agaacatgaa actgttatac tagattgtgt aacattatta gtaaataatc taatgtttga
    21901 acacaatcta aatatagaag aatctagtca agaagaaata aacaaattag aacaatacat
    21961 aaaagaacaa gttgtcaaac ttattgaaga aatagaaaaa acaaatctat actttgtatt
    22021 tgtaacgaat gagttaggta tgggaattgt tcctgggaat aaacttagta gaatttatgc
    22081 tgatattgta ggtcgtataa atcaatatat agcttcgaaa agtaatgaag tttattttgt
    22141 agttagtggc atagctatga agataaagga gtagtatgca attatgaaaa gatttatttt
    22201 aatactacag tttttgacaa ggatacctat aaaattaaat gtaggatttg atgatgaatt
    22261 ttataagtca atagtatatt ttccacttgt aggttttgtc ataggtatat tatcctattt
    22321 aataggatgg atttctatgc ttttatttga accatttata gcttctataa taataactct
    22381 agctggtgtc ttaattactg gaggacttca tatagatgga ttaggagata catttgatgc
    22441 aatatatagt tatagggata aagaaaagat gcttgaaata atgaaagatt caagattagg
    22501 tacaaattca cttctagcaa ttatgtttgt acttttatta aaggttggat tcgtttacga
    22561 tataataagt aataattcat tgtgggttat aatttttatg ccaatgattg caaggttagg
    22621 agtaatgtta ttgacatata aaactgtgac accaagagaa aaaggaatgg gaaatttatt
    22681 tataggaaaa ttaactacaa gtatgttaat aactgctata atatacacat tattgatagt
    22741 tgctcttatt actaaattta tatttttatt acctaatata gtgttgatta aagtattagg
    22801 ttcgattata gtggtttttg tatttataat attattcaaa aaacatattt ataaaaaaat
    22861 tgacggagta acaggagata tattaggctg tggaatagaa ctttcagagc ttgtatattt
    22921 aatttacata tatttgttaa tttttatgtt tttttaaagt aaaaagtggt gataatatga
    22981 taagattaat attaataaga catgcactga caaatgataa taaaaaagga agattatcag
    23041 gccatataaa tagttgtatt agtgaagagg gcaaactaca aataaataaa attaccagat
    23101 atctgagtaa tgaaaacata gataaggttt atacaacacc atctacacgt actaaagata
    23161 cagtagaaaa aatatcaaaa ttgaaattat tagaaataga agaaaaagaa gctttaagag
    23221 aaattagttt tggagacttt gaaggaagaa cttttgaaga aattaaaatc aaatatccaa
    23281 atgagtttga aaaaatgata aaagaaggta acaactatag atatccaaat ggagaaagtt
    23341 tgatagactc ttataaaaga gtggcaaaag aaattgataa tataatttta gaaaataata
    23401 gtaatttaga tacaaagaca atacttatat gttctcatgc aggaacaata agaaatataa
    23461 taacacattt gatttctggt agttataagt atcactggaa ctttaaaatt gataatgcat
    23521 ctattacagt tttagaaatt gatggtggat ttgctgtaat agataaaatg aattttacag
    23581 acttcatata atttaatcaa aaccaaacat tacaatctgt gtttgtaata tatctgtaaa
    23641 attagcaatg aaaatagaat attagattaa aattaggttc ttaattagat gttaaaaggg
    23701 aaaaaggtta gatgcctttg cagcccccgc tactgtgaaa ccaacgaaac tataaatacc
    23761 actgtcagtt tgatgggaag gttatagaag taggatgaag ttaagccagg atacctgcct
    23821 aatttaattt aaaacaagag gtcttcgggg tgaaagattt ttaattaata tatataaaag
    23881 tcctatcttt agaagtatta ggactttttt taatttgttt tttagtaaat tttaacaagg
    23941 agggatggaa agtggggaaa aaaataatgc tacaaggaac agcttcaaat gtaggtaaaa
    24001 gtgtcttagt agcaggtttg tgtagaatat ttaagcaaga tggttatact gttgctcctt
    24061 ttaagtcaca aaatatggct ttaaattctt ttataactaa agaaggatta gaaatgggaa
    24121 gagcacaagt atttcaagca gaggctgcga aaatagagcc aatagctgat atgaatccag
    24181 ttttattaaa accttctgga aataataaaa gtcaagttat tgtaagaggt aaagttgtag
    24241 gagaaatgcc ttcttctaag tatcatgatt ataaattgga gttagtagat gtgttgaagg
    24301 aaacatttaa tagcttaaat tcaaggtatg atattgtagt tatggaaggg gcaggaagta
    24361 atgcagagat aaacttaaaa gatagagata tctctaatat gggtatggca gaaatagctg
    24421 atgcacctgt aataatagtc ggagatattg atagaggagg cgtatttgcc tcattagcag
    24481 gaacaatgct tttattaaat gaagatgaga aaagaagagt aaaaggtgta ataatcaata
    24541 aattcagagg aaaaaaagag cttttacaag atggtgtaaa gatgctagaa gacatcataa
    24601 aagtacctgt tataggagta gtaccttata cagatataaa aatagaagat gaagacagtg
    24661 taactacgag atttaagaaa aaaatgaata aaagtgatat acacatagaa ataattagaa
    24721 ccccacatat gtcaaatttt acagatttta atatatttga gacacaagaa gatgtaagta
    24781 taagatatgt agactatgga gaatctttag gaaatccaga tatattaata atacctggaa
    24841 ctaaaagtac tatagacgat ttaacatata taagaaaaag tggtcttgaa tcgcaaatca
    24901 aaaatttaca tagtcaaggt aaattgatat ttgggatatg tggtggatac caaatgttag
    24961 gtaaaaaatt aaaagaccct tatcatgttg aaagtgaaat tgaagaagtt gatggaatag
    25021 gtcttttaga tacagaaaca acgtttgaga aagaaaagac tacaacacaa gtagaagcta
    25081 ctattgttga atgtatcagt gaatacatga ataaccttaa gggaaaaaaa gtaaaaggtt
    25141 atgaaattca tatgggaatt acaaagcgag gtaatgatgt aagaagttta gatttaataa
    25201 ctaaaaaact tgataaagaa gtgaattata ctgagggaag tgtaaataaa gaggggaatg
    25261 taattgggac ttatcttcat ggaatatttg atgaaataag ctttacaaga gcaattttaa
    25321 acaatataag aaagaaaaaa ggtttaggag aattagaaag caaagtaaac tcttttaatg
    25381 aatttaagga taaagagtat gataagttgg ctgatttttt aagagaacat ttagatatag
    25441 agaaaatata tgagattatg aaataatgat gacctttatt tataatttag gtaggaggtg
    25501 tattatgaaa aaaattttaa ttgcgggtac taatagtgga gtaggtaaaa caacaatatc
    25561 attaggaata atgcaagctt tggttaagag aaatatgaaa gtacagccat ataaagtagg
    25621 gccagactat atagaccctt catatcacac ttttataact ggaagacatt ctagaaattt
    25681 agattcttat atgttagatg atgaaaaaat aaagtatata gtaaataagt cctcaaaaga
    25741 tgcagatatt tcagtaatag aaggtgttat gggcttatat gatggttttg gaatagattt
    25801 agataattgt acaagttcac atacttcaaa ggtattaaag tcacctgtta tattagttat
    25861 aaatggtaaa tctatggcag catctagtgc tgctatggta cttggatata aggaactaga
    25921 taaagatgta aatataaagg gtgttatagt aaataatgta aagactaata gccattatca
    25981 aataattaag agttctatag aaaaatattg taatgttgaa gtattggggt attttcctcc
    26041 aaataacaaa ttctctttgg aatctcgaca tctaggatta gttcctagtg tagaaatgaa
    26101 atcactaagg gaaaaatttt ataccttagc agatgaaatt gaacactata tagatattga
    26161 cagaataatt gaaatatcag aaagtgatga atttgagagt gattttgatt ttcaaacttt
    26221 gatagaaaat aataaaataa aagaacatgt taataataaa tcaatagcta tagcatatga
    26281 taaagcattc aatttttatt atagagaaaa tatagagctt ttagaggaac ttggtttaga
    26341 aataaactat tttagccctc ttgaagatgc atcagtgcca aatgcagatt atatatatat
    26401 tggaggaggt tttccagaaa tatttggaaa agaattagaa gcaaatgagt caatgagaac
    26461 ttccatatta gaagcacata acaataatat acctatttat gcagaatgtg gaggactgat
    26521 gtacctaggt gagaaactat tgaatcaaga agaacaagaa tttaatatgg ttggaatatt
    26581 tagtggaatt agtaagatga catcttcttt aaagagattt gggtactgta ttggtgtagc
    26641 caaggaaaat actcttttat ctaaaaaagg ggaatctatt aaagggcatg agtttcatca
    26701 ttcaatattt gaaagtgatg aaaaatgtgt atattcaatg agaaaagaaa gagatggaaa
    26761 tatagtagaa gagtgggatg gtggttatag taaaggaaac actttggcta catatcttca
    26821 tactcatttt tataataact taaattgtat agaaaatttt ttgaaaaaag gaaatgaata
    26881 aatgagtatt ttatctattt atataggata tgtgacagac ttaattgtag gagacccata
    26941 ttcttttcct catccagtga gatttattgg taaacttata aattttactc aatgtataat
    27001 aagaaaggtc tctaaaaatg ataggcagtt aaaatttggg ggatttatat tatggcttat
    27061 aaccgttgga ataacatatt taacaactta tgctatagtg aagttattta gttttaatat
    27121 attattgagt attgtagtga atagttttat aatttataca acactagcca caaaatgttt
    27181 aaaagacgaa gcattaaaaa tttataatgt attaaaaact gaagatatag aaaaatctag
    27241 gattcagcta tcttatatag taggtaggga tactactaat ttaagtgaac ctgaaataat
    27301 aagagcaact gtagaaactg ttgcagaaaa tactgtagat ggtataattg cgcctatgtt
    27361 ttatgcattt attggaggag caccacttgc tatggcctat aaagctatca atacattgga
    27421 ctcaacagtt ggatataaaa ataaaaagta taaagatatt ggttttgcat ctgctaagat
    27481 tgatgatata gccaattata taccagctag aatatctgtc attcttatga caattggaag
    27541 tttcttttta aattataact ataggaactg ttttaaaatc tctattagag atagaaagaa
    27601 tcataaaagt ccaaattgtg ctttttcaga aggtgcagta tctggtgctc taggaataca
    27661 actaggaggt accaacgttt attttgggga aaaagtatat aagccaacaa taggggataa
    27721 gcttagggag atagataaag aagatataat taaaactaat aaaattatgt atgcatcttc
    27781 atttgtatct atgttggtat ttacactaat atatgtgtta tgtaggatga ttttaattaa
    27841 cttaattata taaattattg tttgttaact taagggggac atagatgaag gatttagggc
    27901 atggtgccaa tgtagatgaa atggcaagat tatatgggaa aaacccaaaa gaaataattg
    27961 attttagttc aaacataaat ccaaatgtat taccaaattt agagagatac attttaaaag
    28021 gattagaaga gtgtaggaat tatccagata taaattatac aaacttgaga gaaaatatat
    28081 caaaatatat agatataaat ccagacttta taatacctgg caatggtgca acagaagtga
    28141 tttatttgct tatgaagagt attaaaaaaa aattggctat aataaatccg actttttcag
    28201 agtatagaag aagtgctgag ttaaataatt tagatattat agatttagaa ttagatttag
    28261 aaaataactt taaacttaat atagatataa taaaagaaaa tataaaaaga tttgatagtt
    28321 tatttatatg taatcctaat aatccgagtg gtaatgtcca agatttaaag gaacttgttc
    28381 atttactaga taaacataat aaggtactaa taattgatga aactttcatg gaatttgttg
    28441 aagatgaaag taaatatagt ttggttaaat atatagaatc aaataaaaat atatttataa
    28501 ttaaagctgt gactaagttt tttggtatgc ctggtttaag gctaggatat ggtcttacaa
    28561 gcaacactga aattatgaac aaaatttatg aacacaaaga gccatggact atcaattctt
    28621 ttgcagatat actatccaat tttatttttg aagataaaga gtatattaaa aatagtaaag
    28681 aatattacat agaagaaaga aaatatatgt tacaagagtt gagaaacata agaaatataa
    28741 aggtctatga tacagatgct aattttatct tgataagaat atataaaaaa acaactaaag
    28801 aattgaaaaa agatttgttt aagcaaggaa acatattagt tagagatgcg tctaatttta
    28861 taggattaga tgatagtttt ataagagtgg ctataaaatc tcatgaagat aacaaaattc
    28921 ttatagaaaa tatgaaaaat ttattgggtg attaaaatga aatcttatgg aatatgccca
    28981 gcatcttgtg gagaatttgt acaaggtata attgatgatg aagaatattt atgttcatat
    29041 gcaatagata tgtattctaa agtgtatatt gaagaaaaat tagtagatat aaatttaggg
    29101 agatataagt caaggttagc aattgaaaag gtatttgaaa aatttaattt acctaaaaaa
    29161 tatactaaaa atatttcttt aaatataaat agtaaaatac ctgttggaaa aggaatggca
    29221 agttcaactg ctgatatagg agctactata aaggcaacat tatcattgat agataaagat
    29281 ttaagtagcg aggagatttc taaattagca gcagaaatag aaccaacaga ttctatattt
    29341 atagataaaa atagtatatt taatcctcta aatggaactg ttataaaata cctaggaaat
    29401 ctgaccaatg cgaaagttgt aatacttgaa ccaaacaaag tactagatac tatgaagatt
    29461 agattgaggc aagattataa taaattaaaa gtagagaata aagaagtaat aaaaaaatca
    29521 tttgctcttt tagaagaagg attgaaaaaa aataatttat ctctagttgg agaggcttgt
    29581 actttgagta gcttagcaaa tgagaatata gaaaaaaaag agtgtttaaa tgaaataata
    29641 aaaatatcaa aaaaatatgg agcatatgga gtaaatattg ctcacagtgg aactgtagta
    29701 ggtattttaa ttgataagtc tatgaatgat aaaaagatga tagatgcctt atgtgaatct
    29761 aacataaatt ctgtctataa taaaatttat acacaaaaca taattaatgg aggaattaag
    29821 ggggaaatag aatggaatac ataaagaatc ctatgaaaat agaagaaaaa agttttgaat
    29881 tgatacaaga aataatagac gaaattagac cagattataa atttaagaat aatatcgaag
    29941 aaaaaataat aaaaagagct atacatacta ctgctgattt tgattactta gatatattaa
    30001 aaatatcaga agaagcagtt gatagtataa tagatgcatt aaaaaataat gcaagtattt
    30061 atactgatac aaatatggct cttagtggta taaataaaag aaagttagaa tcattaggat
    30121 gtaagtataa atgtttagtg gcagatgatg aaactgtaaa attagctaaa gaaaaaggaa
    30181 ttactcgttc tatggcagca gtagaaattg cagctaaaga agaaggaaga aaaatattcg
    30241 ttttaggtaa tgctccaaca gctttatata aagttatgga gatgaaatca gaaggtagat
    30301 tagatttaga tgcagttata ggagttcctg taggttttgt aggagcacaa gaatcaaaag
    30361 atgaagttga aaaaacagat ataccatata taatttctaa aggtagaaaa ggtggcagta
    30421 atttagcagc agcaatagta aatgctatat tatatactat gtaggtacct atatggaaga
    30481 atatgtatat atagatggaa aaaaatatag aagaggatat actacaggtt catgtgcaac
    30541 aggtgcatct aaggctgctg tatatatgtt gattacaaaa aatagaatca atactataaa
    30601 tatagatact cctaagggga taccattact actaaaagtt gataatataa atatttctga
    30661 tacatttgtg gaatgttcca taaaaaaaga tggtggagat gatatagatg caactcatac
    30721 catggatatt tatgcaagag ctgaaattgt agctaaaaat gataagaaca aagggtatct
    30781 tactctaaag gatatagata gtttaagcac caatagtgag tgtaaaagtg agctctataa
    30841 gtttattagg gtttatggcg gaactggtat tggtgtggtt acgaaaaaag ggcttagtgt
    30901 ggatgttggt aagcctgcta taaatccgac tccacttaaa atgataaatc atgaaataag
    30961 aaagcttata ggagataatt ttgaatctat acttggaaat gataaagttt taaaaataac
    31021 aatctttgct ccacaaggag aaactgtagc aaaaaaaaca tttaatccaa gattaggtat
    31081 agttggagga atatctataa taggtacaac tgggattgtt gaacctatga gtgatgaggg
    31141 ttggaagaag tctctatcta tagaacttca gatgaaaaaa gaacaaggct tagataaaat
    31201 aatattggtt ccaggaaatc atggagaaca gtttataaga gaaaaattaa acttagatat
    31261 taagtatgta gttagggtaa gtaattttat aggctatatg attaaagaag ctcaaagaat
    31321 aggatataag aaaatcttaa tggcaggtca tataggtaaa tttataaaag tatctgctgg
    31381 tatttttaat actcatagta aggtagcaga tgctagaagt gagatattag tggcaaactt
    31441 agctttaatg ggagctagat acgaattttt aaataaaata aatcaatgcg taactactga
    31501 agaagctgta gagctaataa ataatagtga atatagagaa gtatataata tattaagtaa
    31561 taaatgtagg gagagagtaa agcaatactt gaatgaagat tcagatgata ttgatgtgga
    31621 agtaatcata ttttcaatgg ataagtcttt attaggtaaa tcggataata cagatgattt
    31681 agtggaggtc tttatatgat aaacataata ggtctaggac ctggcaactt agactatata
    31741 acaaaaaaag gtgaaaattt aatttccact tctgatgtat taattggtgg taaaagaaat
    31801 ttagagtcta taaaaaattt tgaaggagaa aaaatagttc tggattctaa tttaagagaa
    31861 attatagagt acataaataa caataaagaa aagcaaatat caataatagc atctggggac
    31921 ccattaatat atgggatagg tagatatcta tctaaaaata ttgataataa gatgttaaac
    31981 atggtttctg ggattagttc attacagtac atattttcaa aaatatatgt agatatgaat
    32041 gatgtatata ttacaagtag tcatggtaaa gtaccagatt ttgattacat attatcacat
    32101 aaaaaagtat gtatggttac agattcaaag ataggaccaa aacaaataag tagagaaata
    32161 atagatagaa atctaaataa aattattgtt gtaggagaaa atctgtcata tgacaatgaa
    32221 aaaataacaa tagcaaaacc agaagagata ataagaatag acaattttga tatgaatgtt
    32281 gtggtgattt tagatgagga atagtgaatt tataacaggt aaagttccaa ttactaagga
    32341 agaggttaga gcaatatcaa taagtaagtt aaatttagta aatgctaaaa acctcataga
    32401 cataggggct ggtacaggaa gtgtaagtgt tgaagctgca tataattatc ctaatttaaa
    32461 ggttatatct atagagaaaa atgatgatgc tatagagctt atagagaaaa atataaaaaa
    32521 attcaactta aaaaatatag aagtgataaa aggatgtgct ccaattaatc tagggttaaa
    32581 tgttaaagtt gagtctattt ttcttggggg aacaggaaat aatctggaag aaataataga
    32641 atggtctaaa aaaaatctcg ttacaggagg aagacttgta gctaatttta taataataga
    32701 tacatttaat caaactttaa aactgcttaa aaagcatgga tttaaagaaa tagatgtatg
    32761 tgttttgaat gtttctaaac tggagaaact aggaaaaggt gaatatttta aaccactaaa
    32821 tcctatatat ataatatctt gtgaaaaagg ggaaaatgat gatgaataaa gtacattttg
    32881 taggagcagg acctggagat aaagagctga taacattaaa aggatacaaa ttattaagta
    32941 atgcagatgt agtgatatat gcaggttcac ttgttaatcc tgaacttttg gaatactgta
    33001 aagaagattg ccaaatacat aatagtgcac atatggattt acaagagata atagatgtta
    33061 tgagagaagg tatagaaaat aataaatctg ttgtaagact gcaaacaggt gatttttcta
    33121 tatatggttc aattagagaa caagtggaag atttaaataa attgaatata gattatgact
    33181 gtactcctgg agtaagttct tttttagggg cagcatcatc tttaggggtt gaatatacag
    33241 ttccagaaat ttctcaaagt gtaattataa caagaatgga aggcagaact ccggttccag
    33301 agaaagaatc aatacaatca tatgctaagc atcagacatc tatggtaata tttttatctg
    33361 ttcaagaaat tgagaaagtt gtgtctaaat tactggaagg tggatatcca aaagatactc
    33421 ctatagcagt tatatacaaa gcaacttggg cagatgaaaa aatagttaaa ggaactttaa
    33481 gtgatatagc tgtaaaagtt aaagaaaaca acataaataa gactgcttta ataatggttg
    33541 ggagattttt aggagaagag tataataatt caaagttata tgataaggat ttcaaacatg
    33601 aatatagagg gtaatattgc tataatctgt attacagaaa atggaaaaaa attagctcta
    33661 aagataaatt ctctagtaaa taactgtgtt atatatcaag taaaaaataa aaaaagtaat
    33721 ttagttgttg aagaaacatg tgtacataca gttcaaaaga agttaagtga ttttgtagga
    33781 gaaatattta gtaaatttga ttacatagta tttataatgg ctacaggtat agttgtacga
    33841 agtatagcac catacataat aagtaagttt agtgaccctg caattttggt aatggatgaa
    33901 aaaggcaaaa acgtaataag tttattgagt ggtcatattg gaggggcaaa cagcttgaca
    33961 cagtacataa gtagtcttat tgatgctaat ccagtaataa caacagctac tgatataaat
    34021 caaaaagctt ccttagatat gatagcaaaa aaactaaatg cacatattga taattttaga
    34081 gaaagtgtaa aagaagtaaa ttctatgcta gtgaataata aatgtgttgg attatatgtt
    34141 gatgaaggat atcaagttga tactagggga tttaaactta tagaaagtaa ggatgagtta
    34201 agagatagtt tttcaaacac atatatagaa aaattagttg tagtaagcaa taaaataaat
    34261 tgtattgaag atattgagaa tttaaaccta gataataaat ttgaacattg ctatagtacc
    34321 caaaaaataa taaaggtaat tccaagagat atagtgatag gaattggctg tagaagaaac
    34381 acagatagtg aactgatgta taattcactt gaagagtttc ttaataaaca aaatatagat
    34441 attagagcaa taaacacagt tggaagtata gaactgaaaa aagacgaaaa agcaataatt
    34501 gaattatcaa aaatattgaa ggtgccattt aaaacatttt cagtagatga aatatctaag
    34561 gttgataatc tttttgataa atctgatttt gttaaaaaaa atgtgggtgt gtattcagta
    34621 gcagaacctg tagcatatct tttgagcaaa ggtaatttgt tagttgaaaa acataaatat
    34681 aaaggaataa ctttttcagt ggggagaata aaaatatgat atacgttgta ggaattggac
    34741 caggaagtaa agatacaatg actttagaag caataaaagc aatagaagat tctgaggcta
    34801 ttgttggata taagacttat ataaaactaa tagaagaatt tatacaaggt aaagaaataa
    34861 tccaaaatgg tatgaggcaa gaagttgata gatgtaaaca agcagtggag gaagcaaaaa
    34921 aaggaaaaaa agtagcagtt gtaagcagtg gtgatgctgg aatatatggg atggcaggtc
    34981 ttattttaga attaatatct aaagaagatg aagatataaa agtaaaggta attccaggcg
    35041 ttactgcaag tattggtgca gcagctatac ttggagctcc tattatgcat gatttctgtc
    35101 atataagttt gagtgattta atgactccgt gggaagtaat tgaaaaaaga ttgagattgg
    35161 ctgctgaagc cgattttgta atttgtttgt ataatccaag aagtaaggga agaagtgaac
    35221 atctaagcaa agcattcaaa ataatgggtg agtttaaagg tggagataca cctgttggag
    35281 tggtaaaaga tgtaggtaga gaaaaagaag aaaagtttgt atgtactttt gaaaatatgg
    35341 actttgaaaa agtcgatatg actacaatgg ttataatagg aaataagtct acctatatta
    35401 atggtgaatt gatgataact ccaagaggtt atacagtatg attttaattt taggaggaac
    35461 ttcagatagt ttatctatat gtgataagat aaatgaactg aaaaatcaac catatatcct
    35521 ttcagttact acagattatg gaagggaact tgcacaacaa catgcagaga atgtaatttt
    35581 aggtaagctt ggaaaagaag atatgctaaa atttataaaa gataataata tagtaaaaat
    35641 aatagatgcc actcatcctt atgcagttga agtatccaaa actgctatta cttgtgcaaa
    35701 actattgaat atagattata taaggtttga gagaaaatct ttaattgaag aaatagatta
    35761 tgataataaa tttatagtag atacaattga agaagcctgt gaaatagcaa ataaaatagg
    35821 gacaaatata ttcataggaa caggaagtaa aaatttgaac agatttatag aggaaataag
    35881 tgataaaaat ttagttgtta gagttcttcc aacgagtgaa gtcatattaa gttgtgaaaa
    35941 attaggttta agtgcagata atatagtagc tatgaaaggt ccatttagcc agtatataaa
    36001 tgaagaaaca tataaacatt acaatataga tttagtcata acaaaagaaa gtggaatagc
    36061 tggtgggttc ttagagaagg taaatgcatg taaaactttc aatataccag taataattat
    36121 aaaaagagaa tcgattaact acgaaaatgt atttaatgac ataaataaaa ttaaagacat
    36181 acttgtgtaa tttttaggag gagaatatat gaagaaagcc ttattagtag tgagttttgg
    36241 tacaagctat catgaaacga gagaaaagac aatagatata tgtgaagata aaataaaaaa
    36301 ttcgttaaaa agccatgatt tttttagagc ttatacatca aatatgataa taaataaaat
    36361 aaaaaaaagg gatggaatag aaatagataa tccaatacag gctttagata aaatatatga
    36421 acaaggatat gatgaagtaa taatccaaac acttcatatt atatgtggcg aagaatttaa
    36481 caaattaaaa gaacaagtag atggatattc ttctaaattc aaaaagattg tattaggaag
    36541 acctttgctt acacatattg aagattatca ggaagctgta gaggcaatta agcatcaaat
    36601 tcctcatatg gaaagtaatg aagcagtagt atttatggga catggaacac ttcatgaatc
    36661 acattcagcg tatccagcat tggaatacat gcttagggat aatggaataa atgcttatgt
    36721 tggtacagtt gaagggtatc cagaaataga acatgtaata agaagattaa aagaaggaaa
    36781 tataaaaact gtaaatttga tgccatttat gttagttgca ggagaccatg ctataaatga
    36841 tatggcagga gatgaagaag attcttggaa aacaatatta gaagaaaatg gatttaacgt
    36901 aagagttcat ttaaaaggac taggagaaaa ttcttatata caagataaat ttgttcgtca
    36961 tgctgtaaaa tgtgcagaga atgcgaagtt ctatggtata ggagcagggc caggagatgg
    37021 ttctttactt acgataaaag cggttaatac tttaaagaaa ctggatattc tatacactcc
    37081 agaatcaaaa aagggtggag atagtctagc tttatcaata gttagtgaat atttacctga
    37141 atcagtggaa ataaagtcta ggcatttccc aatgagtttt gatgggaatg aaaaaagttt
    37201 agcttgggac aatgtggcac aagaaattat atcagatgtt aatgatggta aaaatgtagg
    37261 atttgttaca ttaggagacc ctatgattta tagtacatat gtatatataa tgaaaagaat
    37321 attaggagag gtagaagtcg aaactatacc aggaatttca tcattttcca atatagcatc
    37381 taatcagaat tttccattag tcatggataa agaagcttta gtggtaattc cttgtactat
    37441 ggaagatgat aagatagagt atgctttgca aaattataat tcaatagtat tgatgaaagt
    37501 atataaaaat tttaaagaaa taataaaaaa attagaaaag tatgatttaa tagaacatgc
    37561 tattttagta agtaattctt cccaagaaag cgaaaatgtg tttacagatt taaaagaagc
    37621 gcaccttgaa gataagatat cttatttttc tactatacta gtaaataaag ataataaaat
    37681 aaaataggtg aaagcaatat ttaaaggaga cttaatatga atatagtggt tggaactagg
    37741 ggtagtaatc tagcgcttat acaaacagaa tgggtaataa atgaattaaa aaagaagtat
    37801 ccagaaatta gttttgagat taaaatcata aagacaaaag gagatttaat tcaaaatgtt
    37861 tcactggata aaataggtga taaaggattg tttgtcaaag aaatagagca acaattatta
    37921 gatggaaaaa tagacatagc agtacatagt atgaaagata tgccttctta tttagcagat
    37981 ggactaaaat ttgctcatac gcctaagaga gaagacccaa gagatgttct aatacttaga
    38041 gaaggatata aaaatttaga tgatttgcct catggagcag taataggaac aggaagtaaa
    38101 agaaggaagt ttcaattatt gaaacaaaga cctgatttaa atatagtaca agttagaggt
    38161 aatgtggaaa ctaggatacg aaaaataaaa gatgaaaaca tgcatggtat tgtgttggca
    38221 gcatcaggaa taattagagc taatttacaa gataaaataa gttcttattt acctgttgat
    38281 gttgttattc cagcacctgc ccaaggagct ttagctatag aaattagaag caatgatagt
    38341 acgatagagg gaatagtaaa ttcactgaag gatgagaata cagaaatcca aatattggct
    38401 gaaagaggtt ttttagatgg tgtaaatgga agttgtcata ttccaatagc agcttattgt
    38461 gaaataatcc aagataaaat acatcttact ggcttatatg gagattcaga aggaaaaaaa
    38521 gtagtaatca agtctataga tggagatatt tcttctccta gagagttagg tttaaagtta
    38581 gctaaacttg tattgaagga gtatgagaat tatgaagggt aaggtttact tagtaggggc
    38641 aggaccaggt gattataaac tcatgacttt aaaaggttta gagtgtataa gaaaatcgga
    38701 tgtaatagta tatgatagat tagcaaatag tagttattta agagaagcta agcctgattg
    38761 tgaacttata tatgtaggca aggcatcaag taatcatatt ttgccacaag aagatataaa
    38821 tagaattata gcagaaaaag ctaaagaagg aaaaattgta actcgtctta aaggaggaga
    38881 cccttatgta tttggcagag ggggagaaga agcagagact ttagtagatg aaggtataga
    38941 atttgaagta gttccaggta taacttctgc tataggaggt ttatgttatg cgggtattcc
    39001 tataacacat agagaccatg cttcgtcatt tcatgtaata acaggtcatt tgaagggtga
    39061 tgatagtgga gaattaaact ggaatgctct tgcaaataac aaaggtactc ttgtgttttt
    39121 gatgggaatt tcaaacctag agaaaataag tgaaaactta atgaaagaag gaaaagataa
    39181 ggatacacct gttgctctta taagttgggc aactagatat aatcaaagag ttgttacatc
    39241 tacacttgaa aatgtttatg aaactgctat taaagaagaa gtaaaaccgc caactcttat
    39301 agtagttggg agtgttgtag gacttagaga aaagttgaac ttctttgaaa gtaagccttt
    39361 atttggaaaa agtattgctg taactaggtc tagaaatcaa aatagtgtat tggtagaaaa
    39421 aataatggat ttaggtggaa atcctataga aatacctact attaaggtag aaaaaataca
    39481 aaataatatt aacttagaaa atgaaataaa aaatataaat aagtataatt atctaatact
    39541 aactagtaaa aatgccgtag aaatcttctt tgaaaagata tatgaaatga attttgattt
    39601 aaggatatta tctaatttaa aggtatgtgc tataggttct gctacatcaa atgagttgaa
    39661 aaaaagagga attatagcgg atatagtacc aaaaaaattt gtagcagagt cactgtatga
    39721 agagttagcg cctattttaa atgatgacga taatatactt atacctagag cagaaaatgc
    39781 tagagattat ctagttgaca aacttaaggc tatttgtgaa gtaacagaag tacatatata
    39841 tagaacagta atagatgagg agaaaaaagg tgagatatta gatattttaa attctaatga
    39901 catagattat ataacattta ctagttcctc aacagtaaat aatcttgttg aaattatagg
    39961 aaaagaaaat attaaaatat tagaaaaaat aaaaacacta tctataggtc ctataactac
    40021 agagacaata aatagtttgg gaataaaatt gtacaaagaa tctgctacat ctactataga
    40081 ttcgttagta gatttaatta taaaaaatta attttggagg cttgaatatg ttaagaagac
    40141 caagaagact aagagcaagt aaagcaatca gaaatttggt aagagaaact aaggtgaatg
    40201 tagaagactt aatatatcca ctgtttgtag tagaggggga aaatataaaa gaagaaatta
    40261 aatctttacc agatgtctat catttatcaa tagatatgct agaagacgaa ataaaagaaa
    40321 taagtgagtt gggaattgaa catataatgt tatttggtgt tccagatgaa aaagatgagt
    40381 gtgcttcaca agcttttaat gataatggaa tagttcaaaa ggcaattaga aaggtaaaag
    40441 aaataaattc agatataaat gtaataacag atgtctgtat gtgccaatat acaagtcatg
    40501 gacattgtgg gattttaact gatactggtt atgttgataa tgataaatca ttggagtatt
    40561 tggctaaaat atctgtaagc catgctaaag caggagctga tatggttgct ccatctgata
    40621 tgatggatgg aagaatacaa gcaataagag aagctttaga tgaaaatgga ttagaaaatg
    40681 ttggaatcat ggcttatagt gttaagtatg cttcaaatta ttatggacct tttagagagg
    40741 cagctcattc agcaccatct tttggagata gaaaaacata tcaaatggac ccagcaaata
    40801 gcaatgaagc tataatagag actgaacttg atatattgga aggtgcagat atacttatgg
    40861 ttaaacctgc attatcatat ttagatatta taagaagagt taaagataat tttgacttac
    40921 ctctagcagc ttataatgta agtggagaat actctatgct taagtttgct gtgaaggaag
    40981 gtttattaaa tgaagatgta attcttgaat caattacatc tataaaaaga gcaggagcag
    41041 atataataat aacttatttt gctaaagaca ttgctaaact gttgaaaaaa taaattctag
    41101 tagtataaag aatatcaaaa agtataggaa ttgtaattaa ctattataca gtagttttgt
    41161 ttagacattt attgaagaat aatagaaact aatttgcaat ttctatactt tatttatgta
    41221 actaaataaa atttggaggt agaagatgct atatcctatt aatttaaaat tagatgagtt
    41281 agatgtagtt ataattggtg gtggagaagt tgcatatagg aaatgtaaaa atttcttaga
    41341 gtttaacaaa aatgttacta tagtttctaa acaaatttta aataaatttt atgatttaaa
    41401 aggaaatata aaaataataa aagatgatta taaagaaata tatataaaag atgcttctgt
    41461 aataattgct gccacaaata atagagagtt aaatatggaa ataggattat attgtagagc
    41521 aaaaaataag ctagtgaatg tagttgataa tatagaaata tcgaacttta ctgttccatc
    41581 atatataaaa agaggagatt tacttataag tgtgtctact gggggcaaga gtccttcatt
    41641 atcatcaaag ataaaaaaag aaattgaaga gagatatact gaagattatg aggagtattt
    41701 aaatgtatta ggagatataa gaaaaaaggt tataaaaaaa tacgaagata aaagtaaaag
    41761 aaaagatgtg cttaatatgt tggtaacact tgacctagaa gaacttaaga agtttgatat
    41821 ataaaaaata aaataaaaaa tgttaaaaag gtgttgacgg attattttta ag
//
LOCUS       DAGSRM010000028        41108 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.028, whole genome shotgun sequence.
ACCESSION   DAGSRM010000028 DAGSRM010000000
VERSION     DAGSRM010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 41108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41108
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.028"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            186..1103
                     /locus_tag="MJE33_001614"
     CDS             186..1103
                     /locus_tag="MJE33_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBY9647554.1"
                     /translation="MMILIQSRGKMKCKELSEELEVSERQIKSYKMYLEQAGIFINST
                     PGIYGGYEIDKCNSISLIKLLDSEVSILDMINSQLEYNNDIYKNEFNNIVEKIKAVLN
                     TGEKSDTYMDYFTVQAQCNCDYESEKNKCNEIIRAYTTKHKFWIEYYSLNSGNSERIV
                     HPYGLFNYKSDTYMVAFCEKRFKFIDFKLCRIKDYKVLEEKYNVDKSFSWDEYSKNSI
                     GIYKGEEISVVIKISHPFSTIIKEKVWVNNQQIIEYDDKSIMFKAKMRGYEEIKSWIL
                     SMGAYVEVVEPDRLRNDILLEIEKMKKIY"
     gene            1181..1918
                     /gene="cas6"
                     /locus_tag="MJE33_001615"
     CDS             1181..1918
                     /gene="cas6"
                     /locus_tag="MJE33_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBY9647555.1"
                     /translation="MKMKIEFSTGCIPISYNSLFMSIIKEAIRKSNEDYYKNMYYYKE
                     KNNKKTKNFTFSVYIKKYSIEGDNFIIEDKVILNISTPDLELGFHIYNGLMTSKKCLY
                     KDYELTRIRIDLSREKKVTEERVLFNALSPICVKSKEGKFLEITDDRYIEELNYITNE
                     VVKNYRGNGLKRKLEFENIGLKKVVVKESLREFKKITGKEYQYINGYKGKFILKGDID
                     DLNLIYNLGIGFRRAQGFGDVDILEWR"
     gene            1924..3801
                     /gene="cas8a1"
                     /locus_tag="MJE33_001616"
     CDS             1924..3801
                     /gene="cas8a1"
                     /locus_tag="MJE33_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas8b1/Cst1"
                     /protein_id="HBY9647556.1"
                     /translation="MKLRVYRGDWFFNMGIVGFLNIIKKADKQAEIFIMEDYIEFDSL
                     FLENFHEYYFNYFMDEYDVSKRIKKNIDYSINFIKSKPDRIKDGIKKIKDSVKQQNDK
                     IKKFDEEKFNLIKEKLDSMSKIKSYDEFDSLEALVDETIDIFKIKSINDRLTANLYKY
                     VVQDNYFGQVSFFNVAKAKLDLDGLKQVMFNDYLRQIIYFGELEDLLEENDYDKLKNY
                     LNDRLNSIAKDINEKKVSKSSINTIEKIMKEINKNFIKKNKSIEDIKKYMDSLEVCEM
                     CGLYKGILDEYSESNFAPLGVSTNNARNMFWNQAYVPSICDICKLILFCTPAGATYTR
                     KNYLINEENEFYLFVNMDTCINELFERNNSLKAQKSEYSDSKEENPFNQLIKSIVEEN
                     TLKSEWQLRNILFVEFKASIDSKKCKINYFNIPTYLAKFFTDKYANKKIQSIYDYKLK
                     SNVLDLLLKNRDLKHLINNILRNKVKNDMESNNKSNISGIDCFRVVQLRALINSYKKG
                     VYKMDSKNLEKNDEKLRIIYYLGCDIHDYFVNKNSKNKIGGVSYKLLNSVKVGNKSDF
                     MDTIIRVFMSAEKQMPAFILDIEIEKDLDFESIGHAFISGLISGKYEGKDKLPNEKEE
                     K"
     gene            3803..4717
                     /gene="cas7i"
                     /locus_tag="MJE33_001617"
     CDS             3803..4717
                     /gene="cas7i"
                     /locus_tag="MJE33_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HBY9647557.1"
                     /translation="MKRGLTFTVIAQAQSLNYGEGIGNISELKKLSRDDGNIYTFASR
                     QCLRYDIVRLAAELFNWNLQVVDKEKGTIQFKDNISIRESEEMDLFGYMKTNKKDEKD
                     KKGGSLTREATVRLSNAISLEPYRSDMDFLNNKGFADRIGEHPNLANIEQHLSYYTYT
                     VTIDLSKIGKDGEIELDNKEKCRRVVEFLEIIKVLNRNIRGRQENLSPLFVVGGVYEI
                     ANPFFLGRIKLKGDKNGFKINKQAIEEVIQGTFLGKDLKEFTYVGMVDGVFINKEEFK
                     GLFEDNFFSVDKFFGQLVKEVKEYYGVN"
     gene            4719..5420
                     /gene="cas5b"
                     /locus_tag="MJE33_001618"
     CDS             4719..5420
                     /gene="cas5b"
                     /locus_tag="MJE33_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5"
                     /protein_id="HBY9647558.1"
                     /translation="MKVLKLDLFQETACYKKPFAMKITETYPLPPYSTINGLIHKILN
                     ATEYIPFNISVQGTYESIFNNYQTTYFYKKDSITSMPMNSHMLLNVNLIIHIGGDFDL
                     LEEIYDSLLNYDEHLSLGRKEDLVRINDIRFVEVNKFEVDEDIQLDDYENYKLSECNI
                     RMPIYIPKTSLQDTDIDGISYRLNNYYDNNAVEDKKRVWNKVDSYYVEDGNTISIGDI
                     LLDNEGDLLYFNNIN"
     gene            5431..7752
                     /locus_tag="MJE33_001619"
     CDS             5431..7752
                     /locus_tag="MJE33_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBY9647559.1"
                     /translation="MLYAKSNPVETLREHTDELLKQMNVLRESYGKNINSLDFLEEEI
                     FWQLLDLVIEFHDIGKAFSPFQELIKSRMDTKINEPKIVTHLENNVGHNYLSPAFIDY
                     SYIDRKKNKELRAVLNQVIVYHHERDIFIDKDFKNLIQKILDEDLINKVYELQHEFKV
                     RYPIKTERLSKVYLQSVEKRIDKNHKYYNLYIMLKGILHRLDHSASAHEVVECNNVIN
                     IGEQTENYLMKEFGSLREAQSFAKSNRNKNIILIASTGMGKTETALIWIDKDKAFFTL
                     PLRVSINALFDRAKNIVGVGESNDTFLGLLHSTAIDYLEESNQENSSEIVDLAKLLSC
                     KLTFSTIDQIFKFPFLYRGYEKVYSTLAYSKVVIDEIQAYSPEIAAVLVKGIEMIHKI
                     GGRFMIMTATMPTIYIDELKKRGVMNSNLAELTCNTEKIRHCVSIVENSIDENLGKII
                     QSGMNKKVLVIVNTVKSAVEKYELIEEIVKPKGIDVNLNLLHSMYIQEDRAKLEKYIK
                     EFANSDRNGIWITTQLVEASLDIDFDELHTENSTLDSLFQRFGRCYRSREYEENSPNI
                     FIYTEKATGIGSIYDKDIVEKGLELLKTFINGKECVKMKEKYKVEMVKILYSKESLEG
                     TAFEKRFTSALNILDTITPYSLGSKDAQNILRNIEGYTVIPEDIYNKIEETLIKDYEE
                     LGQELINAYSNDDRQLINKIKSKRKKARREIIKKTVNLPIYKAKQNVIDIQVKGLEDL
                     KILLYKYDIYENKHTKQIFGKGVLISNEIDQFI"
     gene            7767..8318
                     /gene="cas4"
                     /locus_tag="MJE33_001620"
     CDS             7767..8318
                     /gene="cas4"
                     /locus_tag="MJE33_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HBY9647560.1"
                     /translation="MWLKFTKEGDFVPIDLEVLKVQGVKINYYYVCKRKLWLFSKGIT
                     MEQNSSRVLSGKIVHEDSYKRMKNKEILIDDILKLDIIKDEYVKEVKISSKMKEADEM
                     QLYYYLYYLKNSMGIEKKGTINYVKEKKQEELILTEEKERIIENTLININEINMQLSP
                     SKIEKKHYCTKCAYYEFCFVKEE"
     gene            8321..9301
                     /gene="cas1b"
                     /locus_tag="MJE33_001621"
     CDS             8321..9301
                     /gene="cas1b"
                     /locus_tag="MJE33_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1"
                     /protein_id="HBY9647561.1"
                     /translation="MYKDYYIISNGDIKRKENTIYYYNLEGEKKILPVEQVDDIHIYG
                     EVDLNTKLINYISKYGVMLHFYNYYGYYSGTYYPRKKNVSGFSLVCQSACYLDKNERL
                     YLAKSFVESASYHILRNMRYYKVDESLIDTIKDELNDLNDVKEISELMGKEGRIRKTY
                     YKSFNQILKDDFEFYKREKRPPKDPVNALMSFGNSIMYTNVLSEIYKTQLDPTISFLH
                     EPSTKRFSLSLDIAEIFKPIIVDTIIFSLINNKRINKNDFEYQDKICYLSEKGKKKFL
                     QEFENKMQTTIKHRNLNRKVSYRMFIRLECYKLIKHFINDERYKVLKVWW"
     gene            9304..9570
                     /gene="cas2"
                     /locus_tag="MJE33_001622"
     CDS             9304..9570
                     /gene="cas2"
                     /locus_tag="MJE33_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HBY9647562.1"
                     /translation="MFVIVTYDIVEARSLNRIRRILRKYLTWTQNSVFEGNITEGKLH
                     KCISEIENIIDNSEDSIYVYEIKNPNSIKKKCYGIDKYSDEMFI"
     repeat_region   9754..11555
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=9754..9782
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaat"
     gene            11744..12172
                     /locus_tag="MJE33_001623"
     CDS             11744..12172
                     /locus_tag="MJE33_001623"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBY9647563.1"
                     /translation="MIKKLNNKDINKIMEIWEKSTIKAHDFISKEYWQNNYNTVKNEY
                     IPISDTFVYDDGDEIKGFISIIDKSFIGALFIKPKYQNLGIGGKLLDYATKKYKSLSL
                     AVYKDNKKAVVFYNKKGFNIVKEQVNEDSGFKEYIMEYSK"
     gene            complement(12627..13406)
                     /locus_tag="MJE33_001624"
     CDS             complement(12627..13406)
                     /locus_tag="MJE33_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647564.1"
                     /translation="MKVNENNINNIDEELGELSRKVYLESPDFWISISDKIIDKVFDE
                     MLLFFAVKYNYFNIVCFAIENNYLDLNMPSKNKKYTNIMEHLLDTSKQNEDKKIYNYL
                     LSLNKGNNLDERPILDELNCNISSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDIT
                     YKFSSKEAEPIEMCSEVSSIRCCNCDELLEDITLDKLYNLCKIQNCNNCGSNLTKVGI
                     IAKKKMNFDENTEKFKDVSTSYCCGNCDSEISLSQREHFKI"
     gene            13730..14275
                     /locus_tag="MJE33_001625"
     CDS             13730..14275
                     /locus_tag="MJE33_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBY9647565.1"
                     /translation="MKNTKVNIQDLTKMSICVALLCISSYIYIPLPFTPAGVTAQTIM
                     INLIALILTPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFYFGFLFAALI
                     ISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAAVVPFLIGDT
                     IKSVIASFLGTKLNNVLRRNL"
     gene            14307..15443
                     /locus_tag="MJE33_001626"
     CDS             14307..15443
                     /locus_tag="MJE33_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HBY9647566.1"
                     /translation="MKEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKDLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKVKSGQ
                     CDLIIAGGFESSSLQPMRTYHKNDKRYDTNNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIEKADLDFWVKESHKRAKEAREEKILEDIISPINNSTYDEGIRDKMSQRLLDRMP
                     SILGKETITNAANSCLINDGASFIIICSKKYLEHVKKKPKAKIINTCTIGTDATLSPT
                     SAIQAMDKLLEIESLNYLDVSAVEFNEAFAVIDVLFQRKYPELIDRYNIFGGALAYGH
                     PYGASGAIIALHLLKALEKTKGRYGICSIAAAGGLGSALLLERV"
     gene            15447..16838
                     /locus_tag="MJE33_001627"
     CDS             15447..16838
                     /locus_tag="MJE33_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HBY9647567.1"
                     /translation="MNYYELLRIKKERQKNKNFLIIDGEKYTYENILSYSEELGKQIS
                     LGENIPILIYSKNIMFQLISFFAINYSKNIPIICHYNLSKKVLDDILLKNNISIIISD
                     ESMDVIDLYDDNNNEVAKKVNFSIFNPKFNIYMYQYKKLINYLDKSICMGALSSGSTS
                     VPKVLYRTYESWAGFFPIQNGIFNISGESILFVNGTLSFTGNLNSIMSVLYEGGSIVI
                     SSGLNCKSWIRTIKQYNVTNIYLIPTKLQLLVKHLKEPVLKVVSIFTGSQLLFEDTAR
                     NLKKYMPKSEIILYYGASELNYITYLCYDELIEKPLSVGRPFPGIDIYIREGKIFVNT
                     EYAVYNATKPYSVNDIGYYDNDGYLIFEGRSDDIINIGGFKVSSTKIENEVKKIPQIE
                     DAIVLPYSDTIRGNQIVLFVITIDKITKKDLLIKMKDNLMKNEIPKKIIFLNSFPYTS
                     SEKIDRLALLKML"
     gene            16914..17789
                     /gene="dpsA"
                     /locus_tag="MJE33_001628"
     CDS             16914..17789
                     /gene="dpsA"
                     /locus_tag="MJE33_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HBY9647568.1"
                     /translation="MSNQYDITVIGGDLRQVYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDDCLDATIDNLLINLSPNQILFGGNI
                     PTKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAIAEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYALDANVCVGARNNDARSTANAFGYSSISLSQLDEKLLQFDYIFNTI
                     PECILTKERLEKVSQEVTIIDIASSPGGVDYSVVKEAGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            17792..18382
                     /locus_tag="MJE33_001629"
     CDS             17792..18382
                     /locus_tag="MJE33_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HBY9647569.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEEAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
     gene            complement(18589..18789)
                     /locus_tag="MJE33_001630"
     CDS             complement(18589..18789)
                     /locus_tag="MJE33_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647570.1"
                     /translation="MEKLKTISVFSLIISVILTIGGIGIVTYYVDNLFIRGLSVFVLI
                     MSSSFVSTTVRLIFEESKRYKF"
     gene            19293..21947
                     /gene="adhE"
                     /locus_tag="MJE33_001631"
                     /gene_synonym="adhC"
     CDS             19293..21947
                     /gene="adhE"
                     /locus_tag="MJE33_001631"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBY9647571.1"
                     /translation="MAHKEQVIIENKKENEVMSVGLDTVNNVESLVKKLAKIREAQKI
                     FATYSQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KETKTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDDSADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     NIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDDAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     ENVGVKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVALKELKDVMNKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKYTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYTNGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYAE
                     IASFLGLKGNSDEEKFQSLLVAIEDLKLKVGIPKSIKEFGVEESKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
     gene            22223..24442
                     /locus_tag="MJE33_001632"
     CDS             22223..24442
                     /locus_tag="MJE33_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBY9647572.1"
                     /translation="MTGRNKSFTLITILFLTSLFLTMGYIYIEDTKHLLMSEAKIHIK
                     EVAVQGSQLAQRQIEKDLDVLNIFSNYYASNPDIPNEEKMKNLLDEMENQKFYTMAIV
                     DINGNAENTKGDKFSVKDREFFKNSIKGKKYVSSPYVDEVNKSIKKIAISVPLLNNDK
                     VVGVLYCTYNINTLMKLINISFYENNSISYVVKNNGTIILHPQGDSLSKNIYKLLKQD
                     NDIQEVNRLKKELQENKTGATVLNMLEERRYLGYATMDNGNSENNYIKDWNLIFSIPE
                     TVVFSNSKQIINRAVYAVLSIVLIFIAIIFYIIYIKKSNEKEILSLAYEDKVTYIGNQ
                     NKFYRECSKYLLDKPSLNYIIVYFDINNFKMINDTFGYEFGDNLLITIAKALKEELTE
                     GEVYARLSSDYFAIFCDYKNGRNKIIRKLDNIRSNIESNLSIVFEISLCVGIYFVEEG
                     EVDIQKAVNKANMARSVAKGKNINYAIYNEDVRNKLSEESMILDDIKIALVKNQFEVY
                     YQPKFSLVTGEMIGSEALIRWNHPEHGFISPAVFIPIAEKSKLILKIGRFVFERVCND
                     LYEWKKQGKKIVPVSVNLSRVELYQPDIVKFINKTIKMYNLSSDFIEIEITETVAINE
                     LNILKNVLNELRTYGFSISMDDFGTGYSSISCLRDMPIDILKLDKSFLGGIEHDERSR
                     NIAKSIVSLAKSLDLVVIIEGVESKEQAELMKQFGCDLVQGFYFARPMPAKNFLDLL"
     gene            24696..25358
                     /locus_tag="MJE33_001633"
     CDS             24696..25358
                     /locus_tag="MJE33_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HBY9647573.1"
                     /translation="MIDISIDDKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TINQSLKVEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSQESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            25729..27138
                     /locus_tag="MJE33_001634"
     CDS             25729..27138
                     /locus_tag="MJE33_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBY9647574.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGVLFVAYIGMAIFFRNHFYF
                     GTTINGVKASGKTVEQVNDLLTSSTNSYTLNLEERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMITLSYNKDLLEKRFNALSCFDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEAPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     GLSKKYSTFGDSRPFKTATGKDITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNKDDIGNTYVEISLSGQHMWFFKNGEMLVSTDVVTGDLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGSIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            27253..27528
                     /locus_tag="MJE33_001635"
     CDS             27253..27528
                     /locus_tag="MJE33_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HBY9647575.1"
                     /translation="MNEKLTKILEEAKNDDEFRKELIKTREQRDVMDSFCKVVTSRGY
                     DMTVGELFSMGEEYTSNLLKSVNGGAAYPIEDWSDWYEDFFIMLSNI"
     gene            28158..28469
                     /locus_tag="MJE33_001636"
     CDS             28158..28469
                     /locus_tag="MJE33_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647576.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VKKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAEERKSKAIEAVIGKVV
                     R"
     gene            28471..30396
                     /locus_tag="MJE33_001637"
     CDS             28471..30396
                     /locus_tag="MJE33_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBY9647577.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEGQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVNFDIPLENMETKYTKTILGTVP
                     ISANIDALRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHADSIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFNCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVVFFGMMLSDAAYGLIIAVVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGILGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVIDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            30399..30890
                     /locus_tag="MJE33_001638"
     CDS             30399..30890
                     /locus_tag="MJE33_001638"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBY9647578.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            30942..31505
                     /locus_tag="MJE33_001639"
     CDS             30942..31505
                     /locus_tag="MJE33_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HBY9647579.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            31520..32497
                     /locus_tag="MJE33_001640"
     CDS             31520..32497
                     /locus_tag="MJE33_001640"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HBY9647580.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKIMSSAILEAFDVYNKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            32484..32807
                     /locus_tag="MJE33_001641"
     CDS             32484..32807
                     /locus_tag="MJE33_001641"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBY9647581.1"
                     /translation="MLMYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVED
                     EYAIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGAD
                     ILFKE"
     gene            32924..34702
                     /locus_tag="MJE33_001642"
     CDS             32924..34702
                     /locus_tag="MJE33_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBY9647582.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEMHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTTKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            34699..36072
                     /locus_tag="MJE33_001643"
     CDS             34699..36072
                     /locus_tag="MJE33_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBY9647583.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            36078..36746
                     /locus_tag="MJE33_001644"
     CDS             36078..36746
                     /locus_tag="MJE33_001644"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBY9647584.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDTAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSDAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            37023..38072
                     /locus_tag="MJE33_001645"
     CDS             37023..38072
                     /locus_tag="MJE33_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBY9647585.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADIIWGLSAMNMIELKK
                     DGALEAYKPKNYDKVDKKFKDTEDDVSWVGMSVPETAIVVNYKELKKLGIDIPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGEKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPIEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYAHEVAVTSIKTDNPVPKGYSKQPFEQLIKEVDLNSAAKNRSTV
                     LKTWESKYGSKSESK"
     gene            38168..39157
                     /locus_tag="MJE33_001646"
     CDS             38168..39157
                     /locus_tag="MJE33_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBY9647586.1"
                     /translation="MSYLKINNVFKSYDQKRVLNNISLDIEEGEFLCLLGPSGCGKTT
                     LLRIIAGLEDVNSGTIILQDKDITNLEPSKRGFGIVFQSYALFPNMTAYNNIAFPLKE
                     RKVSKGKIDNKVKEVLETVGLTNEAHKYPKALSGGQQQRIAIARALALEPKFLLLDEP
                     MSALDAKVRHKLRMDIKRLQKELNITTIMVTHDQEEAITMADKIAILNGGDIMQIGTP
                     EEIYQNPQNLFTAQFIGDTNCFDNGDSILTVRPEYVQIEKSTKENYQGIISNIEFRGN
                     LLRVEIKDKLNENFIISDASIKEWVNLNLVEGDLVKISIDEKYYLKYPKVKGA"
     gene            39162..40880
                     /locus_tag="MJE33_001647"
     CDS             39162..40880
                     /locus_tag="MJE33_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBY9647587.1"
                     /translation="MNNNAISSKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASESLLNSLKNTVFVSVISTVISLTLALVYAYALTR
                     TKIKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLVTNLLGVHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLSSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDSILGGVVGIIAFGIIAVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLLTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFNFMDTIYIKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAASLAVLILVTNILVKVIYEFIKNRLE
                     KRTNFN"
     gene            complement(40962..>41108)
                     /locus_tag="MJE33_001648"
     CDS             complement(40962..>41108)
                     /locus_tag="MJE33_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBY9647588.1"
                     /translation="NSTNELKLSMSIGYAIFDEKVDVSLFDTYRRADSQMYDNKRKIN
                     KQYQ"
BASE COUNT        16288 a         4455 c         7147 g        13218 t
ORIGIN      
        1 attaactatt aaaattttgt acaaatataa aatataatat ggtaaatgtc aaaattataa
       61 aaaaattcta cttatataaa atataaagag gttatatgta catatgtgta gaaataaata
      121 ataaaaatat caattaatgt tattaggggg ggttactcat tagtaaatta gctaatacac
      181 ttagaatgat gatattaatt caaagcagag gcaaaatgaa atgcaaagaa ttatcagaag
      241 agttggaagt aagtgaaagg caaataaaaa gttacaagat gtatttagag caagctggta
      301 tatttataaa ttcaacacct gggatttatg gtggttatga aatagataaa tgtaatagca
      361 tctcattaat taaattatta gatagtgaag tttcaattct agatatgata aattcacagt
      421 tagagtataa taatgatatt tacaaaaatg aatttaataa tattgtagaa aaaataaaag
      481 cagtattaaa tacaggagaa aaaagcgata catatatgga ttattttaca gttcaagcac
      541 aatgtaattg tgattatgag tctgaaaaaa ataagtgtaa tgaaataatt agagcatata
      601 ccacaaaaca caaattttgg atagaatatt attcattaaa ttcaggaaat agtgaaagaa
      661 ttgttcatcc atatggttta tttaattata aatcagatac ttatatggta gctttttgtg
      721 aaaaaagatt taagtttata gactttaaat tatgtagaat taaagattat aaggttttag
      781 aagaaaaata taatgtagat aaaagtttta gttgggatga atatagtaaa aatagtatag
      841 gaatttataa gggagaagaa attagtgttg ttataaaaat tagtcatcca ttttcaacaa
      901 ttataaagga aaaagtttgg gttaataatc aacaaatcat agaatatgat gataaatcta
      961 taatgtttaa agctaaaatg agaggttatg aggagataaa aagttggatt ttaagtatgg
     1021 gagcttatgt tgaggttgta gaaccagata gacttagaaa tgatatattg ttagagattg
     1081 aaaaaatgaa aaaaatttac tgattttttc aaataattta tacttggacc aatatacttc
     1141 acggtctagt gttaatatat taattgagag aggtgatatt atgaaaatga aaattgaatt
     1201 tagtacaggt tgtataccaa tatcttataa ttcgctcttt atgagtatta taaaagaagc
     1261 aataagaaaa tctaatgaag attactataa aaacatgtac tattataaag aaaagaataa
     1321 taaaaaaact aaaaatttta cattttcggt ttatataaaa aagtatagta ttgaaggtga
     1381 caattttatt atagaagata aagtaatatt aaatataagt actccagatt tagaattggg
     1441 ttttcatata tacaatgggt taatgacttc taaaaaatgt ttatataaag actatgaact
     1501 tacaagaatt agaatcgatt tatcaagaga aaagaaggta acagaagaaa gggtactatt
     1561 taatgcattg tctccaattt gtgtaaagtc taaagaagga aaattcttgg agataactga
     1621 tgatagatac atagaagaac taaactatat aactaatgaa gtggttaaaa attatagggg
     1681 gaatggatta aaaagaaagt tagaatttga aaatataggt ctcaaaaagg tagtagttaa
     1741 agagagttta agagaattta aaaaaataac aggtaaagag tatcaatata ttaatggtta
     1801 caaaggtaaa tttatattga aaggtgatat tgatgattta aatcttatct acaatctagg
     1861 aattggtttt agacgtgctc agggatttgg agatgtagat attttagaat ggaggtgaga
     1921 taaatgaagt taagagttta tagaggagat tggtttttta atatgggtat agttggattt
     1981 ttaaatataa taaagaaagc agataaacaa gctgaaatat ttattatgga agattatatc
     2041 gaatttgata gcttatttct tgaaaacttt catgagtatt attttaatta ctttatggat
     2101 gaatatgatg tatcaaaaag aattaaaaaa aatattgatt atagtataaa ttttattaaa
     2161 tctaagcctg atagaatcaa agatggaatt aagaaaatta aagatagtgt gaaacaacaa
     2221 aatgataaga ttaaaaaatt tgatgaagaa aaatttaacc tgataaaaga gaagctagat
     2281 agtatgtcta agataaaaag ttatgatgaa tttgacagtt tagaagcttt agtagatgag
     2341 actatagata tatttaaaat aaaatcaata aatgacaggc ttacagcaaa tctttataaa
     2401 tatgtagttc aagataatta ttttggacaa gttagttttt ttaatgttgc taaggcgaaa
     2461 ttagatttag atggattaaa acaggtaatg tttaatgatt atttaagaca aattatatat
     2521 tttggagagt tagaagattt attagaagag aatgactatg ataagttgaa aaactattta
     2581 aatgatagat taaatagtat agctaaggat ataaatgaaa aaaaagttag taagtctagt
     2641 ataaatacta tagaaaaaat tatgaaggaa ataaataaaa actttataaa gaaaaataaa
     2701 agcatagaag atataaaaaa gtatatggat agtttagaag tatgtgaaat gtgtggttta
     2761 tataagggaa tattagatga atactcagaa agtaattttg ctccacttgg agtaagcacc
     2821 aataatgcta ggaatatgtt ttggaatcag gcgtatgtac catcaatatg tgatatttgt
     2881 aagctaatat tgttctgtac tccagcaggg gctacttata caagaaagaa ttatttaatt
     2941 aatgaagaaa atgagtttta tttatttgtt aacatggata cttgtataaa tgagcttttt
     3001 gaaagaaata atagtttaaa agctcaaaaa agtgagtatt cagattccaa agaggaaaat
     3061 ccttttaatc agcttataaa aagtatagta gaagaaaata cattgaaaag tgaatggcaa
     3121 cttcgtaata tactttttgt ggaatttaaa gcaagtatag attctaaaaa atgtaaaata
     3181 aattatttta atattccaac ttatttagca aaatttttta cagataagta tgcaaataaa
     3241 aaaatacaaa gtatctatga ttacaagctt aaatcaaatg tactagattt actcttgaaa
     3301 aatagggact tgaagcatct aattaataac attttaagaa ataaagttaa aaatgatatg
     3361 gaaagcaaca acaaatcaaa tatatcagga atagattgtt ttagagtagt ccaattaaga
     3421 gcattaataa atagctacaa aaagggggta tataaaatgg atagtaaaaa tttggagaag
     3481 aatgatgaaa agctgagaat aatttattat ttaggttgtg atatacatga ctattttgtt
     3541 aataaaaatt ctaaaaataa aataggtgga gtaagctata aacttttaaa ttcagttaaa
     3601 gtcggaaata aaagtgattt tatggataca attataagag tttttatgtc agctgaaaaa
     3661 cagatgccag catttatttt agatatagaa attgaaaaag atttagactt tgaatctata
     3721 ggtcatgctt ttatatctgg attaatttct gggaaatatg aaggaaaaga taaactacca
     3781 aacgaaaagg aggaaaaata agatgaaaag agggttaaca tttacagtaa tagcacaagc
     3841 tcaaagttta aactatggtg aagggatagg taatatatca gaattaaaaa aattaagtag
     3901 agatgatgga aatatatata catttgcatc tcgccagtgt ttaagatatg acatagtacg
     3961 attagcagca gaattgttta attggaattt acaggttgtc gataaagaaa aaggtactat
     4021 tcaatttaaa gataatataa gtataagaga atcagaagaa atggatttat ttggatatat
     4081 gaaaacaaat aaaaaagatg agaaagataa aaaaggaggt tctttgacta gagaagctac
     4141 tgtaagatta agtaatgcga tttctcttga gccatataga agtgatatgg attttttaaa
     4201 taataaagga tttgcagata gaataggaga acaccctaat cttgctaaca tagagcaaca
     4261 tttaagttac tatacatata cagtgactat agatttatct aaaataggca aggatggaga
     4321 gatagaactt gataataaag agaaatgtag aagagtagta gaatttttag aaataataaa
     4381 agtattgaat agaaatatta gaggtagaca agaaaattta tctcctctat ttgtagtagg
     4441 aggagtatat gaaatagcaa atccattttt cttgggtaga attaaattaa aaggagataa
     4501 aaatggattt aaaataaata aacaagctat agaagaagta atccaaggta cttttttagg
     4561 aaaagattta aaagaattta cctatgttgg aatggtagat ggggtattta taaataaaga
     4621 agaatttaag ggattgtttg aagataattt ttttagtgta gataaatttt ttggacaact
     4681 agttaaagaa gtaaaggaat attatggggt gaattaatat gaaggttttg aaattagatt
     4741 tatttcaaga aactgcatgc tacaaaaaac catttgctat gaagattaca gagacatatc
     4801 cactgcctcc atattctaca ataaatggat taattcacaa aatattaaat gctacagaat
     4861 atataccatt taatataagt gtacagggaa catatgaaag tatatttaat aattaccaga
     4921 caacatattt ttataaaaaa gatagtatta catctatgcc aatgaatagt catatgctat
     4981 tgaatgtaaa tttgataatt catataggtg gggattttga tttattggaa gaaatatatg
     5041 atagtttatt aaattatgac gagcacttat cattaggtag aaaagaagat ttagtaagaa
     5101 ttaatgatat tagatttgta gaagtcaata aatttgaagt agatgaggat atacaattag
     5161 atgattatga aaattataag ttatcagaat gtaatataag aatgcctata tatataccaa
     5221 aaacaagttt acaagataca gatatagatg gtataagtta cagactcaat aattactatg
     5281 ataataatgc tgtggaagat aaaaaaaggg tttggaataa ggtagatagt tattatgttg
     5341 aggatggaaa tactatatca attggagata tattattaga taatgaagga gatttattat
     5401 attttaataa tataaattag gagggagatt atgttatatg ctaaatctaa tccagtggaa
     5461 acacttagag aacatactga tgaactttta aaacaaatga atgtattaag agaaagttat
     5521 ggaaaaaata taaatagctt agattttcta gaagaggaga tattttggca attacttgac
     5581 cttgttatag aatttcatga tattggaaag gcattttcac cgtttcaaga gttaataaaa
     5641 agcagaatgg atactaaaat aaatgaacct aaaatagtaa ctcatttaga aaataatgta
     5701 ggccataatt atctttctcc tgcatttatt gattatagtt atatagacag gaaaaaaaat
     5761 aaagaactta gagcagtatt aaatcaagtt attgtatatc atcatgaaag agatatcttt
     5821 atagataaag attttaaaaa tttaatacaa aaaatattag atgaagattt aataaataaa
     5881 gtttatgaac tacagcatga atttaaagtt agatatccaa taaaaacaga aagattgagt
     5941 aaagtatatc tacaaagtgt agaaaaaaga atagataaaa atcacaagta ctataattta
     6001 tatattatgt taaaaggaat actacataga ctagaccata gtgcttctgc tcatgaagtt
     6061 gtggaatgca ataatgttat aaatatagga gaacagacag aaaattattt aatgaaagag
     6121 tttggtagtt tgagagaggc tcaatcattt gctaaatcta atagaaataa gaatataata
     6181 ctgattgcat ctacaggaat gggaaaaaca gaaactgcct taatttggat agataaagac
     6241 aaggcatttt ttacacttcc attaagagta agtataaatg cattatttga tagagcaaaa
     6301 aatatagttg gagttggaga atcaaatgat acattcttag gtttgttaca ttcaacagca
     6361 atagattatc tagaagaaag taatcaagaa aattcaagtg agattgtaga tttagcaaag
     6421 ttattatctt gcaaactgac attttctaca atagaccaaa tatttaaatt tccttttttg
     6481 tatagagggt atgaaaaagt gtactcaaca ttagcatatt caaaagtagt aatagatgag
     6541 atacaagcat attcaccaga aatagcagct gtattggtaa aaggtataga aatgatacat
     6601 aagataggtg gcagatttat gataatgaca gctactatgc caaccatata tatagatgaa
     6661 ttaaaaaaaa gaggtgtaat gaatagtaac ttagctgaat tgacatgtaa tacagaaaaa
     6721 ataagacatt gtgtttctat tgtagaaaat tcaatagatg aaaacttagg taaaataata
     6781 caaagtggaa tgaataaaaa agttcttgta atagtaaata cagttaaaag tgctgtagaa
     6841 aaatatgagt taatagaaga aatagttaaa ccaaaaggaa tagatgtcaa cttaaatctt
     6901 ttacattcta tgtatataca agaagataga gcaaaattag aaaaatatat aaaagaattt
     6961 gccaatagtg atagaaatgg aatttggatt acaacacaat tagtagaggc atctcttgat
     7021 attgattttg atgagcttca tacagaaaat tcaacacttg acagtctatt tcaacgattt
     7081 ggtagatgtt atagaagtcg tgagtatgaa gaaaatagcc caaatatatt tatatataca
     7141 gaaaaagcta caggaatagg tagtatttat gataaggata ttgtagaaaa aggacttgag
     7201 ctactgaaaa cttttataaa tggaaaagaa tgtgtgaaga tgaaagaaaa atataaggta
     7261 gaaatggtta agatactata ctctaaagaa agtctagaag gtacagcttt tgaaaagaga
     7321 tttaccagtg ctttaaatat tttagatact ataacgccat atagtttagg ttctaaagat
     7381 gcacaaaata tacttagaaa tattgaagga tatacagtaa ttccagagga tatatataat
     7441 aaaattgaag aaactttaat caaagattat gaagagttgg gacaagagct tataaatgca
     7501 tatagtaatg atgatagaca attgataaat aaaataaagt ctaagagaaa aaaagcaaga
     7561 agagagataa ttaaaaaaac tgtaaattta ccaatatata aagctaaaca aaatgtaatt
     7621 gacatacagg tgaaaggtct tgaagattta aaaatacttt tatataaata tgatatatac
     7681 gaaaataagc atacaaaaca gatatttggt aaaggtgtgc ttataagtaa tgaaatagac
     7741 caatttattt gatttttata ttaacaatgt ggctaaaatt tactaaagaa ggtgattttg
     7801 tgccaataga tttagaagta ttaaaagttc aaggtgtaaa aataaattat tattatgtat
     7861 gtaaaagaaa actatggcta ttttctaaag gaataaccat ggaacaaaat agtagtagag
     7921 tattgtctgg gaagatagta catgaagatt catataaaag aatgaaaaat aaagaaatat
     7981 tgattgatga tatcttgaag ctagatataa taaaggatga gtatgtaaaa gaagtaaaaa
     8041 taagttcaaa gatgaaagaa gcagatgaaa tgcagttata ctattactta tactatttaa
     8101 agaatagtat gggtatagag aaaaaaggaa ctataaatta tgttaaggaa aagaaacaag
     8161 aagaattgat attgacagaa gaaaaagaaa gaattattga aaacacactt ataaatataa
     8221 atgaaataaa tatgcagtta tctccttcca aaattgagaa aaaacattat tgtacaaaat
     8281 gtgcttatta tgaattttgt tttgtgaagg aggagtaaca atgtataaag actattatat
     8341 tataagtaat ggggatataa aaaggaaaga aaatacgatt tactattaca atttagaagg
     8401 tgagaaaaaa atattaccag tagaacaagt tgatgatatt catatatatg gagaagtaga
     8461 tttaaacaca aaacttataa attacataag taaatatggg gtgatgcttc atttctacaa
     8521 ttactatgga tattattcag gtacatatta tccaaggaag aaaaatgtat caggattcag
     8581 tttagtatgt caatcagcct gctatttaga taaaaatgaa agattatact tagcaaaatc
     8641 ctttgtggaa agtgcatcat accatatttt aagaaatatg agatattaca aagttgatga
     8701 aagtttaatt gatactataa aggatgaact aaatgatttg aatgatgtca aagaaatatc
     8761 agaacttatg ggtaaagaag gcagaataag aaaaacatac tataaatcat ttaatcaaat
     8821 attaaaagat gattttgaat tttataaaag agaaaaaaga cctcccaaag acccagtaaa
     8881 tgctcttatg tcttttggaa atagcataat gtatacgaat gttttaagtg agatatataa
     8941 gacacaactt gaccctacaa taagcttttt acatgaacca tcaacaaaga gattttcttt
     9001 aagtttagat attgcagaaa tttttaagcc aattatagtt gacacaataa tttttagcct
     9061 gattaataat aaaagaatta ataagaatga ttttgaatat caagacaaaa tttgttattt
     9121 aagtgaaaaa ggaaagaaga aatttttgca agaatttgaa aataagatgc agacaactat
     9181 aaaacataga aatctaaata gaaaagtttc atatagaatg tttataaggt tagagtgtta
     9241 taaattaatt aaacatttta taaatgatga aaggtacaaa gttttaaagg tgtggtggta
     9301 agtatgtttg ttattgttac ttatgatatt gttgaagcaa ggtcgttaaa tagaattaga
     9361 aggatactta gaaaatattt gacttggaca caaaattctg tttttgaagg caatattact
     9421 gaaggaaagt tacataaatg tatttctgaa atagaaaata ttattgataa tagcgaggat
     9481 tcaatctatg tttatgagat aaaaaatcct aattcaatta aaaagaaatg ttatgggatt
     9541 gataagtatt ctgatgaaat gtttatatag gtttgcagtg agcgatattt atgctaaaat
     9601 aggtgttaac agttggaata taagggattg aaggtgtatg ataactgtta tcaattgcac
     9661 tactgctcgc tcactgcaaa ttttgatgtt tttattgaat tataattgct tgattgaagt
     9721 gttttcaatg tattcaaata tacctatttt ggggttttat attaactaag tggtatgtaa
     9781 attggaaaac ttgtcaaaaa tagcatcaaa gcatttatag ttttatatta actaagtggt
     9841 atgtaaatgg ggcttttggg gacatgagtg ttacagatgc actaagtttt atattaacta
     9901 agtggtatgt aaatagcccc ctaatgctgg aacagtgagg acacacaaac gttttatatt
     9961 aactaagtgg tatgtaaatg aacttttaaa agattatgct aggaaaattc atgttgctgg
    10021 ttttatatta actaagtggt atgtaaataa gccaaaagct acgaatttga actaaaatgc
    10081 gtgggtttta tattaactaa gtggtatgta aattgcctag ggaacccctc cagcccctaa
    10141 ccatgcaaca gttttatatt aactaagtgg tatgtaaata acaagaaaaa ccaacaacaa
    10201 aaccaacaac aaaaaggttt tatattaact aagtggtatg taaatctttc cataaaatac
    10261 cccctacttc atttttattt agttttatat taactaagtg gtatgtaaat aagaccaagc
    10321 cacttccgca attgcatctt ctaacatgtt ttatattaac taagtggtat gtaaataaat
    10381 attttgttga cttatttcaa actttagagg acgttttata ttaactaagt ggtatgtaaa
    10441 tagtgtattt ttatcattct atctactata ccaaaaaagt tttatattaa ctaagtggta
    10501 tgtaaatgca tacctgtaac ctgcatcatt ttgtatctat cttgttttat attaactaag
    10561 tggtatgtaa ataaaagatt gtaatgagta cgagacaaga tttattgtgt tttatattaa
    10621 ctaagtggta tgtaaattat ataaattttc tttttcttct atttctaagt tcagttttat
    10681 attaactaag tggtatgtaa atgttgtata gctagtactt cctgctttaa atccttttcg
    10741 ttttatatta actaagtggt atgtaaataa gacaaggttc ttcttttaag tggcaagcta
    10801 taaaagtttt atattaacta agtggtatgt aaatcgagta aaataacaat tatctggaat
    10861 gtgagaagtt gttttatatt aactaagtgg tatgtaaatt acacataatt acaacccagt
    10921 ttactttttg ctacacgttt tatattaact aagtggtatg taaattatga atatattaca
    10981 taatcataaa gatttacaaa tggttttata ttaactaagt ggtatgtaaa ttctctaaat
    11041 ccatctataa cgttaccagc aaaatcaatt ttatattaac taagtggtat gtaaataaga
    11101 acttttcatg aacgtggaaa atattgagtt cagttttata ttaactaagt ggtatgtaaa
    11161 tttgtcattt acgactacca tttctctagc atttatatgt tttatattaa ctaagtggta
    11221 tgtaaatggg aaaatatggg aaaagttaac taatataatg caaagtttta tattaactaa
    11281 gtggtatgta aatgaaattt ttagtgctat tgcaactact cttgtttgtg ttttatatta
    11341 actaagtggt atgtaaatgc ataaccaaaa acttcaaaat caagacctcg aatttgtttt
    11401 atattaacta agtggtatgt aaattgcgca ataacatgct gaaaactgca atttcgaagt
    11461 gttttatatt aactaagtgg tatgtaaata ggataaagaa aagactcaca caaggcacag
    11521 tgtcaggttt tatatcaact aagggtagat aagtagaata ttagcaatat caacgagtat
    11581 ttagaaactt attttatatt aataaagcag tatataaaat tgtagaatca acaatagcat
    11641 atactaaaac atccacctta tattaactaa atcatatcta aataaaatac acttacctat
    11701 aaacattata aaatcaatac aaaaatgagg tgaaataaaa tttatgataa agaaattaaa
    11761 caataaagac ataaataaaa tcatggaaat atgggaaaaa agtacaatca aagcacatga
    11821 ctttataagt aaagaatact ggcaaaataa ctataatact gttaaaaacg aatatatacc
    11881 tatatcagat acatttgtat atgatgatgg agatgaaata aaaggattta taagcataat
    11941 agataaaagc tttataggag ctttatttat aaagcccaaa taccaaaatc taggtatcgg
    12001 aggtaaactt ttagattatg caactaaaaa atataaaagt ctaagcttag cagtatataa
    12061 agataataaa aaagcagttg tgttttataa taaaaaaggt tttaatatag taaaagaaca
    12121 agtaaatgaa gattcaggat ttaaagagta cataatggaa tatagtaaat aatatgatta
    12181 cattctatta aaaggataat taatttattt gccatttgat tttttaagag taatttttaa
    12241 aatagactat atgaagataa agatatttat tatagatgta agttaatcag aattgatata
    12301 gctacaaaag aggaaaataa aattgtatgg tgtatttcag atttaacaaa tttactattt
    12361 aaagataata agtttgatat aatattaaag atactttcac ctataaacta tagggtattt
    12421 gaaagaattt taaataatta tggaatgcta ataaaggttt gttaaaattt aataattatt
    12481 agattataga aaattgccat gaaattagtt catcaagaac tagtttcata gcaattttaa
    12541 ttcttataag tgtatttatt tttacagatt taaaaaactt atacagtatc taataatgta
    12601 atcttattac tttagcctat aaatatttaa atcttaaaat gttctctttg agataagctt
    12661 atttcagagt cacaatttcc acaacagtaa gatgttgaga catctttaaa tttttcagta
    12721 ttctcatcaa agttcatttt tttctttgca ataattccta ccttagttaa attagaacca
    12781 caattattac agttttgaat tttgcatagg ttatatagtt tatcaagtgt aatatcttct
    12841 aataattcat cacaattaca acatctgata gaagaaactt cactacacat ttcaataggt
    12901 tcagcttctt tagaagaaaa tttatatgtt atgtcatttg atactttata acctgtatca
    12961 aatatattag aattacaact tggacataag tactttggaa gatatatatc ttttttttca
    13021 aaagtatcac tactaatatt acagtttaat tcatctaaaa ttggtctttc atctaggtta
    13081 ttacctttat ttaagcttag aagataatta taaatttttt tatcttcatt ctgctttgat
    13141 gtatctaaca aatgctccat tatattagtg tattttttgt tttttgaagg catgtttaaa
    13201 tccagataat tgttttcaat agcaaagcat actatattga aatagttata tttaactgca
    13261 aaaaataata acatttcatc aaatacttta tctattattt tgtcagaaat acttatccaa
    13321 aaatctgggg actctaagta aactttccta ctaagttcac ctaactcttc atctatatta
    13381 tttatattat tttcattaac tttcatatca tcacttcctt ttaaatatct tgtgtataaa
    13441 ctatttgtta ctatttttta tataaaataa aatcttatat aaaaatatat catattataa
    13501 tttacaagta tagtctaaat agagctgttt ttaagcaaag ttttataaat gactattaaa
    13561 atataaagta acagtataaa gtttacttat aaattaaatg taaacctaaa taattataat
    13621 agttgacaaa aaaacgtctt tattttatac taaaacaaag ctttaataga agctttgtat
    13681 atgtacatat aaactaaatg aaataagaca tatgaaggga attataaaaa tgaaaaatac
    13741 taaagtaaat attcaagatt taactaaaat gtcaatatgt gtagcattat tgtgtatatc
    13801 atcttacata tatataccat tgccatttac tccagcagga gtaacagcac aaacaataat
    13861 gataaattta atagctttga ttttaacacc aagacaagca tttagtacag ttggtgtata
    13921 tataatgata ggtttaattg ggattccagt atttggtgga ggtacatctg gtattggaaa
    13981 gttgttaagc ccaacaggtg gtttttattt tgggtttttg tttgcagcac taattattag
    14041 tttattaaaa ggacgaaaca atgatattaa gagatatata ctaataacaa tttttgttgg
    14101 aatgcctatt atatatttta tgggaacagt atttatgtgc tattttaatc aaatgacagt
    14161 agaagcagca ctttttgcag cagtagtacc atttttaatt ggagatacaa taaagtcagt
    14221 tattgcatct tttttaggca ctaaactgaa caatgtactc agaagaaatc tatgataaat
    14281 ctataatata aggaagggtg taatgtatga aggaagtttt tatattagga ggattgagaa
    14341 gtcatatagg tctaaaaaat ggaatttttc aatttgttca acctgaatta ctaggagcaa
    14401 gtgttttaaa agatttaatt aaaaagtatg aaattgacaa aattgatgag attatatgtg
    14461 gaaatgcagt tgggacagga gggaatatag ctagattaat gactttaaca gcaggagtgt
    14521 caaatgaagt acctgctttt actgtggata tgcagtgtgc ttctgctatg atgagtatag
    14581 acatagcttt ttcaaaggtt aaatctggtc agtgtgattt aattattgca ggtggttttg
    14641 aaagtagttc tctacaacca atgcgcacat atcataagaa tgataagcga tatgatacaa
    14701 ataatccaaa ttatacagtt gctcaatttt ctcctgatga taatagccaa aatagtatgc
    14761 tcgaaggggc ggaaagagta gcagagctat atcaaattga gaaagcagac ttggattttt
    14821 gggtaaagga aagtcataaa agagcaaaag aagcaagaga agaaaaaata cttgaggata
    14881 ttatatctcc tataaataat agtacctatg atgaaggtat acgtgataag atgagtcaaa
    14941 ggttattaga taggatgcca tcaattttag gaaaagaaac tattacaaat gcagcaaatt
    15001 catgtcttat taatgatgga gcttcattta ttataatatg ttctaagaag tatttagaac
    15061 atgtaaaaaa gaagcctaaa gcaaaaatta ttaatacatg cacaatagga acagatgcaa
    15121 ccttaagtcc aacatcagcg atacaagcaa tggataaact tttagagata gaaagtctta
    15181 attatttaga tgtatctgct gttgaattta atgaagcatt tgcagttata gatgtgttgt
    15241 ttcaaagaaa gtatccagag ttaatagata gatataatat atttggtgga gctttagcgt
    15301 atggacatcc atatggtgct tcaggtgcaa ttattgcatt acatctttta aaagcattgg
    15361 aaaagactaa gggtagatat ggaatttgct ctatagctgc agctggtggt ctaggttcag
    15421 ctttactgct tgaaagggtg tagattatga actattatga acttttaaga ataaaaaaag
    15481 agaggcaaaa aaataaaaat tttttaatta tagatggaga aaaatatact tatgaaaata
    15541 tattgagtta tagtgaggaa ctaggaaagc aaatttcttt aggtgaaaat atcccaattt
    15601 taatatattc taaaaatata atgtttcagt tgattagttt ttttgctatt aattattcaa
    15661 aaaatattcc tattatttgt cattataatc tttctaaaaa agtattagat gatattttat
    15721 taaaaaataa tatttcaatc attatatctg atgaatctat ggacgttatt gatttatatg
    15781 atgataacaa taatgaagta gcaaaaaaag ttaatttttc tatttttaat cctaaattta
    15841 atatatatat gtatcaatat aagaagctta taaattattt agataaaagt atttgtatgg
    15901 gagctttatc ttcaggttct acaagtgttc caaaggtatt atatagaaca tatgagagtt
    15961 gggctggatt ttttcctata caaaatggta tttttaatat ctctggggaa tcaatcttgt
    16021 ttgttaatgg aacacttagt tttacaggga atctaaattc tataatgtca gtattatatg
    16081 aaggaggtag tattgtaata agttcaggtc ttaattgtaa atcatggata aggacaataa
    16141 aacaatataa tgtaactaac atatacctta tacctacaaa acttcaactt ttggttaaac
    16201 atttaaaaga accagtactc aaagttgtaa gtatttttac agggtctcaa ttattatttg
    16261 aagatactgc aagaaatcta aaaaaatata tgccaaagtc tgaaattatt ttatactatg
    16321 gggcaagtga attaaattat attacatatt tatgctatga tgaactaatt gaaaaacctc
    16381 taagtgtagg cagaccattt ccaggaatag atatatatat tagagaagga aaaatttttg
    16441 tgaatacaga atatgcagta tataatgcaa ccaaacctta ttctgtcaat gatattggat
    16501 attatgataa tgatggatac ttaatatttg aaggaagaag tgatgacatt ataaatattg
    16561 gaggatttaa agttagttct actaagattg aaaatgaagt taaaaaaatt cctcaaattg
    16621 aagatgcaat tgtattgccg tactcagaca ctataagagg aaatcaaatt gtattatttg
    16681 taataactat agacaaaatt acgaagaaag atttgttgat taaaatgaaa gataatctaa
    16741 tgaaaaatga aatcccaaag aaaattatct ttttaaattc atttccatat acctcttctg
    16801 aaaagataga tagattagca ttattaaaaa tgttataagt ataatattta gtaattttag
    16861 tgaaccaatg tattgataaa aacatattgt atatagtaaa ggaggcttac aatatgtcaa
    16921 atcaatacga tataactgtt attgggggag acctgcgaca agtgtacatg gtaaaaaaac
    16981 taattaataa agggtattct gtagtcactt atgggatatc aaatgaaatt ataaatggaa
    17041 tcgttggaaa atcaacttca ctagctgaag cactatccaa aagttccatt attttatgtc
    17101 caattccatt tacaaagaat caagttaata ttgaaaatac ggatgattgt cttgatgcta
    17161 ctattgataa cttgctaatt aatttatctc ctaatcaaat attatttgga ggtaacatac
    17221 caactaaagt ttgtaaattt tgcgagcaac actctattca gatttatgat tttatgaaaa
    17281 tgaatgatgt tgctatttta aatgcagttg ctacagctga aggggctata gcagaagcta
    17341 ttaaaagaag tataatcaat atacatcaaa gtaactgttt agtattaggt tatggaagat
    17401 gtgcacaaat acttgcaaaa aaattatacg ctttggatgc aaatgtatgt gttggcgcaa
    17461 gaaataatga tgcaagaagt acggctaatg catttggata ttccagtatt tctttaagtc
    17521 aacttgatga aaaactttta caatttgatt atatttttaa caccatacca gagtgtattt
    17581 taacgaaaga aaggttagaa aaagtatcac aagaagtaac tattatagat atagcttctt
    17641 ctcctggagg agtagattat tctgttgtta aagaagcagg gattaatgcg actctttgcc
    17701 ttgggcttcc tggtaaatat tcaccaaaga catctggtga aattcttgtt aacgctatag
    17761 aaaatattat aaatgaaaga agggattaga aatgagactt gaaggactta caattggagt
    17821 aggatttact ggttcgtttt gtacttatga taaaatattt atagagttag agaatttggt
    17881 taaagaagga gctaatgtac acactatatt ttcagatgta tcacaaaata tcgattgtag
    17941 atttggaaat tctgaagagt ttatgaaaaa agcatatgaa cttacaggaa ataaacctat
    18001 agttacgatt gaagaggcag agccatttgg acctaaagga attgctgata ttataataat
    18061 agctccttgt actggaaata ctgctgcaaa attagcaaat ggtattacag attcaccagt
    18121 attaatggca gcaaaaggtc atcttcgtaa tgataaacca ctagtaatat caatatcaac
    18181 gaatgatgct ctaagcttta atttcaaaaa tattggcatt ctccttaatt ctaaaaatat
    18241 atattttgta ccttttggtc aagataattg caaagcaaag cctaattcta tgattgcaca
    18301 tactgagcta ataattccaa ctatagaact tgctctagag aacaaacagc tacagcctgt
    18361 tataaaaagt ccacatcaat agaattggtt cttataatat tatgtattag atacaatact
    18421 agcaaagagg ctgtctcaaa atagagagaa gcctcttttt cttgttgaaa tgatgaaaat
    18481 atatttaatt tcatacaaaa aaatgcctca tggactaaaa agtctatttt gagctaatct
    18541 ccgtaatttt tgctatatta aaattattta cttaatccaa tttttaaatt agaatttata
    18601 tcgttttgac tcttcaaata tcaatctaac tgttgttgat acaaaagaag aggacataat
    18661 taaaacaaat actgatagtc ctctaataaa caaattatct acataataag ttactattcc
    18721 aatacctcca atcgttaaaa ttacacttat aatcaaagaa aaaacgctaa ttgtttttaa
    18781 tttttccatg cttatcctcc ttcttataaa cttcaaattt atctatataa tattaaatat
    18841 attttatttc atattatata aaaatcatga atatatattt ttttaattat actatatgtt
    18901 gtaaatgttt gtacatttaa ctatatttac tttaacttgt taattaagtt ttaattttca
    18961 ttttcaagga tttttatttt taaacttgat agagtatcat attaaaaatt ctttttataa
    19021 agttttattt gcaaaaaata caaaaatata aacgttaaaa aaataacgtt atccttaagt
    19081 ttttagatga tttcattagt gatttgcgta aaaaataaag tgatatctag ttaaattaag
    19141 tatttgtaaa gctagatatt attagtagaa atgatgttat attagataaa aaataaaatg
    19201 ttaaaaaaat aacttgaaaa taggaaaagt aaatgctata atgttaataa attaacataa
    19261 taaataaaaa actatctcag gaggttgtta aaatggctca taaagaacaa gtaataatag
    19321 aaaataaaaa agaaaatgaa gttatgtcag ttggtttaga tactgtaaat aacgttgaaa
    19381 gcttagttaa aaaactagca aaaatcagag aagctcaaaa aatatttgct acatatagtc
    19441 aagagcaggt agataaaatt tttttagcag catctttggc agctaataag caaagagttc
    19501 ctctagctat aatggcacaa aaagaaactg gtatgggaat tgctgaagat aaggttataa
    19561 agaatcatta tgcctcagag tatatatata atgcatataa agaaactaaa acttgtggtg
    19621 ttatagagaa agacgaagca tttggtatga ctaaaattgc tgaaccaata ggtgttattg
    19681 cagcagttgt acctacaact aatccaactt ctactgctat ctttaaagca cttttagctt
    19741 taaaaactag aaatggaata attttctctc cacatccaag agcaaaaaat tctactatag
    19801 aggctgcaaa agttgttctt gaggctgctg ttttagctgg agctcctgaa ggtataatag
    19861 gatggataga tgagccatct ttagaattaa ctacaactgt tatgaaagaa gtggatttga
    19921 cccttgctac aggaggtcct ggaatggtta aatcagcata ttcatctgga aaaccagcta
    19981 ttggagttgg tgcaggtaat actccagcaa taattgatga tagtgcagat ataaaaactg
    20041 ctgtaaactc aattcttgta tctaagactt ttgacaatgg tatgatatgt gcttctgaac
    20101 aatcggttat agtattagaa aatatataca atgaagtcaa aaaagaattt aaagagcgtg
    20161 gtgcttattt attagataaa gatgaaacag aaaaaataag aaatataatt ttagtaaatg
    20221 gaggattaaa ttctaaaata gttgggcaaa cagcttgtac tatagcaaaa cttgcaggct
    20281 ttgaagtccc agtagatact aaggttttaa taggtgaagt agaatcagtt gaaatagaag
    20341 aagcttttgc tcatgagaaa ctttcaccag tacttgcaat gtacaaggca agtgattttg
    20401 atgatgcagt tagaaaagca gaaaaattag tagaagatgg tggatttggg catacatctt
    20461 cattatatat tgatgatgta aatcaaagag aaaaacttga taagtttact tcagccatga
    20521 aaacttgtag aattttaatt aatactcctt cttctcaagg tggtataggg gacttatata
    20581 attttaaact agctccttct cttactttag gatgtgggtc ttggggtgga aattcagtat
    20641 ctgaaaatgt aggagttaag catttactta atattaaaac agtagctgag aggagagaaa
    20701 atatgctttg gtttagagca ccagaaaaag tttatttcaa aaaaggttgt ttaggagtag
    20761 cattaaaaga acttaaagat gtaatgaata agaagagagc ttttattgta acagatacat
    20821 tcctttacaa taatggatat actaaagcag ttactgattt attagatgaa atgaatatta
    20881 agtacactac attctttgaa gtagaaccag acccaacttt ggaatgtgct aagataggcg
    20941 ctaaagctat gagagaattt aatccagatg taataatatc tataggtgga ggaagtgcaa
    21001 tggatgcagg aaagataatg tggacattgt atgagcatcc agatgtagat ttccaagacc
    21061 tagctatgag atttatggat ataagaaaaa gggtatatac attccctaaa atgggtgaaa
    21121 aagctaattt tgtagctata ccaacatcag caggtactgg ttcagaagta acaccatttg
    21181 ctgttataac tgaccaagat acaggagtaa aatatccatt agcagattat gaactgatgc
    21241 caaatatggc tatagttgat tctgatatga tgatgaatat gccaaaaagc ttaacatctg
    21301 cttctggtat agatgcttta actcatgcat tagaagcata tgtatctatg cttgcaaccg
    21361 attatacaaa tggattagct ttacaagcta ttaagagtat atttgaatat cttccacgtg
    21421 catatgataa tggtgcaaaa gacccagaag ctagagaaaa aatggcaaat gcttctacaa
    21481 tggcaggtat ggcttttgca aatgcatttt tgggtgtttg tcactctatg gctcataaat
    21541 taggtgcttt ccaccatgta ccacatggtg ttgcaaatgc tcttttgata acagaagtta
    21601 tgaaatttaa ttcttcagat gcaccaaaga aaatgggagc attctctcaa tataaatatc
    21661 cagaagcatt aaagagatat gctgaaatag cttcattttt aggattaaaa ggaaattctg
    21721 atgaagagaa attccaaagt ttattggtag ctattgaaga tttaaaactt aaagttggta
    21781 ttcctaaatc tataaaagag tttggcgttg aagaatctaa gtttatggat tcaatagatg
    21841 aaatggtaat tcaagcattt gatgaccaat gtacaggagc aaatccaaga tatccactta
    21901 tgaatgaaat aaaagatatg tatttaaatt catattatgg aagataagtt tgctataaaa
    21961 tagataaaat aaaaactctt atacataaaa caaagtttta ttgtataaga gtttttttgt
    22021 ttgagaaaat ggatagtttt tattctttaa tttaaaattc aattaaatag tgttattata
    22081 ataataaaag aatgttttta tggttattat gttgatttta tgtgtaacat ttaagaatag
    22141 ttatatacaa tataaatgat gaattggtat aaaaaggata tattatctat atgaagtatt
    22201 gactaattag gggaggagtc gaatgacagg gagaaataaa agttttacat taattacaat
    22261 tcttttttta acatcattat ttttgacaat ggggtatatc tatatagaag atacaaaaca
    22321 tcttcttatg tcagaggcaa aaatacatat aaaagaagtt gcagtacaag gctctcaact
    22381 agcacaaaga caaattgaaa aagatttaga tgttttaaat attttttcta actattatgc
    22441 ttcaaatcca gatataccta atgaggaaaa aatgaaaaat cttttagatg aaatggaaaa
    22501 tcaaaaattt tacactatgg caatagttga tataaatggt aatgctgaaa ataccaaagg
    22561 tgataagttt tcagtgaaag atagagaatt tttcaaaaac tcaattaaag gtaagaagta
    22621 tgtttcaagt ccatatgttg atgaagtaaa taagtctata aagaagattg ctatttcagt
    22681 accattatta aataatgata aagtagttgg agttttatat tgtacttaca atatcaatac
    22741 tctgatgaaa cttattaata tatcttttta tgaaaataat agtatatctt atgttgtaaa
    22801 aaataatggt acaattatcc ttcatccaca aggggatagc ctatcaaaaa atatttataa
    22861 attgttaaaa caggataatg atattcaaga agtaaataga ttaaaaaaag aattgcaaga
    22921 aaataaaaca ggtgcaactg tattaaatat gttggaagaa agaagatatt taggatatgc
    22981 cactatggat aatgggaata gtgaaaataa ttatataaaa gactggaatt taattttttc
    23041 aattccagaa actgttgtat ttagtaattc aaaacaaata atcaacagag ctgtatatgc
    23101 agtgttatct attgtattaa tatttattgc aattattttt tatataattt atataaaaaa
    23161 atctaatgaa aaagaaatat taagtttggc ttatgaagat aaagttacat atataggaaa
    23221 tcaaaataaa ttttatagag aatgtagtaa gtatctttta gataaaccat ctttaaatta
    23281 tattatagta tattttgata ttaataactt taagatgatt aatgatactt ttggatatga
    23341 atttggtgac aatttattaa ttacaatagc aaaagcttta aaagaagaat taacagaagg
    23401 tgaggtatat gcacgacttt caagtgatta ttttgcaatt ttctgtgatt ataaaaatgg
    23461 aagaaataaa attataagaa aacttgataa tatacgaagt aatattgaaa gtaatttgag
    23521 tatagtcttt gaaatttctc tgtgtgtggg tatttatttt gttgaagaag gtgaagtaga
    23581 tattcaaaag gctgtaaata aagcaaatat ggctaggtct gttgctaaag gtaaaaatat
    23641 aaattatgct atttataatg aagatgttag gaataagctt agtgaggaat caatgatttt
    23701 agatgatata aaaatagcat tggtaaaaaa tcaatttgaa gtgtattatc aacctaagtt
    23761 ttcacttgta actggagaaa tgatagggag tgaagcacta ataagatgga atcatccaga
    23821 acatggtttt ataagtcccg cagtatttat tccaatagca gagaaaagta agttgatttt
    23881 aaaaattggg agatttgtat ttgaaagggt ttgcaatgat ttatatgaat ggaaaaagca
    23941 aggtaaaaag atagttccag tttcagtaaa tttatctagg gtcgagttgt atcaaccaga
    24001 tattgtaaaa tttatcaata aaacaataaa aatgtataat ttaagttcag atttcattga
    24061 aatagagata acagaaactg tggctattaa tgagttaaat attttaaaga atgttttaaa
    24121 tgagttgagg acgtatggat tttctatttc tatggatgac tttggtacag gatattcttc
    24181 tattagttgt ttaagagata tgccaattga tatattgaag ctagataagt cttttcttgg
    24241 tggcattgaa catgatgaga gaagtcgcaa tatagcaaaa tcaatagtgt ctctagcaaa
    24301 atcactagat ttagttgtaa ttatagaagg tgttgagagt aaagaacaag ctgagttaat
    24361 gaaacaattt ggatgtgatt tagttcaagg attttatttt gcacgaccaa tgccagctaa
    24421 gaatttttta gatttacttt aaaacaaaat ttgattttta tataaagcac tattatgaat
    24481 gcttgatttt aataaatcat tgctaaataa ttagtgtttt tttattttat caatgtattg
    24541 tctaatataa agcaaaaaat aattgtattt tataaaaatt atattaaaaa taaatttatt
    24601 ttgctaataa acatgttatt aacaaaagaa tagtactata atgtatagat gataaaaact
    24661 ttaataaaaa tttagtgtat aggagttgtt taagtatgat agatataagt attgatgaca
    24721 aacttaagga aagatggcct aatgctaagc tgggttgcat tcaagcaaaa gtagcagtaa
    24781 ataaaagctc agaaaaatta attaatgaaa taaatgagtt ttgtgatact ataaatcaaa
    24841 gtctaaaagt tgaagacatt acgaaactag ataaaattaa agatgcaaga aatgcttata
    24901 aagagcttgg aaaaaatcca agtagatata gaacatcttc agaagcattg gttagaagaa
    24961 ttattcaagg gaagggtctt tatacaataa ataatatagt agaaataaac aatttaatat
    25021 caataaaatc attatatcca gtaggtacat ataatgtaag caaattacat tcaccagtat
    25081 gttttactgt aggagatgaa ggagaacagt ataagggtat tgggaaagaa ttaataaata
    25141 tagaaaatct tcctatatta tctgattcag tgggtaaatt tgggagtcca actagtgatt
    25201 ctgaaagagc aatgataaca gaagatgcaa atgaaatatt aatttgtata ttttctttta
    25261 gtcaagaatc tgatattgaa gaatattttg gatatgctaa ggaattactt aaagaatatg
    25321 cagatggaaa agatatagaa actaaaataa ttaaatagaa tctatataac aacgttatgt
    25381 taagctaaaa ttcaatataa aaataagtta agataaagaa actaacacaa aattagtttc
    25441 ttttttaaaa tagtagttta gtgttacaaa aatagataga tagtcccacg attattaaaa
    25501 gtatggtaat atattaatat agcagagatt gtgtttaagg gattttgaaa ggggggattt
    25561 ataataatca ttttatctaa aagataaaat ggttttatag aaataactat atatttaaaa
    25621 atataaataa tgatacagta atttgggtat ataaaataag gcatacatgg ttttgtttaa
    25681 aaaccaataa ggtacatagg taattacaat gtaaggaagg atttaattat gattgatgga
    25741 aaagaggtaa aacaagaaag tggacaaaaa aatctaaaat ttaagatagc tatagtagta
    25801 ggtgttttgt ttgtagctta tataggaatg gcaatctttt ttagaaatca tttttacttt
    25861 ggaacgacta ttaatggcgt aaaggcttca ggaaaaacag ttgagcaagt aaatgatttg
    25921 ttaacatcta gtactaattc ctatacttta aacttagagg aaagaaacaa taagaaagaa
    25981 caaataaaag caaaagatat tgatttaaaa tttacaccag atgataaagt tcaaaagata
    26041 aaagatagtc agagcgctat tggatggatt ggtggaatat ttaagtctaa agagtataat
    26101 aatatgataa ctctttcata taataaagat ttactagaaa aaaggtttaa tgctttatct
    26161 tgttttgata gtaaaaatgt tgtagaacct aagagtgctt atcctaagta tgatagcact
    26221 aaaaacgctt atgttatagt accagaggta cttggaaaca aagtgaaaaa agatgatttt
    26281 tataagttag tacaaaactc aattaattct gggaatactt ctatttctct ggatgaaagt
    26341 aaagtttatg aagcaccaaa caacacaaag gattcagaaa agttgaaaca agctattaag
    26401 acattagata agtatgttgg tgtagtcgtt acatatgatt ttagtgaccg taaggaaact
    26461 ttagatggtt ctgaaattaa taaatggtta aaagtagact cagaaaagaa ttatgacata
    26521 acttttgata atgatgctat ggtagaatat acaagaggtt taagtaaaaa atacagtact
    26581 tttggtgact caagaccatt taaaactgcc acaggtaaag atattacaat ttcaggtgga
    26641 atttatggtt ggttgataga taaaaagaaa gaagctgaag cattagttga tgttgtaaaa
    26701 gctggtaaaa atgttacaag agaacctatt tacatacaaa aagctgtatc tcgtaataaa
    26761 gatgatattg gaaatacata tgtagagatt agtctttctg gccaacatat gtggttcttt
    26821 aaaaatggag aaatgcttgt atcaacagac gttgtaacag gtgatttagc tagaggattt
    26881 gctacaccat caggtatata tcctcttaat tataaggcta gaaatgtttc attaactggt
    26941 caaggttaca gttcacctgt aagttattgg cttccgttta atgggaacat aggcattcat
    27001 gatgctactt ggagaaattc ttttggtgga agtatatata agtcttcagg ttcacatgga
    27061 tgtgtaaata cacctttttc taaggcaaag aaaatatatg aaaatataga accaggtaca
    27121 ccaattattt tatactagta aatgtgttgc taacagttaa gcgaagtcat cttagtttat
    27181 ttaggatggc ttccttttta tataaagctt attgaagttt acagaaagac tacttaaaag
    27241 gagaaataca taatgaatga aaaattaact aaaattttag aagaagctaa gaatgatgat
    27301 gaatttagaa aagaacttat aaaaacaaga gaacaacgtg atgttatgga ttcattttgt
    27361 aaggttgtta catctagagg ttatgatatg acagtagggg aacttttttc tatgggagaa
    27421 gaatatacat ccaatctact gaaaagtgta aatggaggag cagcatatcc tattgaagat
    27481 tggagtgatt ggtacgagga tttttttata atgttgtcga atatatagag taactaatga
    27541 tattatctac ttaagattaa tacattgcct taaaataaaa ctattagaac taggtgtaag
    27601 ttttatcaat ctttaaaaat attgatttat ttctaactag atataaaaaa attagattaa
    27661 aaataaggtg acaaaaatcg atatagcttg atgatttata aaagttagat aaaatagtgc
    27721 gagatatttc taaaaattca aaaaaaacaa taaacttcaa tggttagagg ttgaaaaaga
    27781 atataaaaaa cttttatatg taataaatta aattaaaatt ataaaaaaaa taaaataaaa
    27841 atttaaaatg tcgacataac gttaatttta aaacaaaata acgctattga caaactatat
    27901 cgctttaaat tataattatt gaaaataatg gtatttaaaa accatactca tgtcatagtc
    27961 taaaagtaag tcaaaaaagg atgtaaatct aggcaagtac tattctaaaa tatgttttat
    28021 taagaaataa acgttgaaaa atattaatta ttatataaaa atgctgttta tactttaaca
    28081 atgaggtagt aatctaatta gaataaaaaa atctaattgg ttatatacat agtaaaaagg
    28141 taagagaggt gatttggatg gcttttgaaa taataaaaag cattgttgag gcagagcaga
    28201 cagcagacag tatcaaagta aaagctgtta ctgatgcaga gtctatcaga gctgatgctg
    28261 taaacaaatg tgaaagcata tttgctgatg taaaaaaaca agcaaagctt atggaagaaa
    28321 ctcttattga gaaggcagtc accgacagta gagcagaggt tgataaaatc ttagctaatg
    28381 ctaaaagtga atgtctgaaa attgaaaaaa ctgctgaaga aagaaaaagt aaggctattg
    28441 aagcggttat tggaaaggta gtgagataat atggcaatag ttaatatgaa caaaatatct
    28501 attgttggct tagaagctca aaaaagccag attttaaagc tacttatgaa ccgtgggttt
    28561 gttcagattg atgatagtgc ctttcttact gaggaggagg aattaaaaaa ttatcttgta
    28621 aaagatagtg aagaatctga agttataatg ctagagcaga aaatgtctca tgtaaaccaa
    28681 gcaattaata ttgtttcttc actagtgaaa caaaaaaaat ccctacttgc accaaaaaga
    28741 gagtttaaca aaattgatag tgaaaaagaa gggcaagttt atgctgaagc tctggagtta
    28801 aacaatttgc acaaggagat aggtacaata aggtctaaca taaacttact taacaacaat
    28861 aaaagtatgt tagaaccatg ggtgaacttt gatattcctc ttgaaaatat ggaaactaag
    28921 tatacaaaaa ctatacttgg aactgtgccc ataagcgcaa acattgatgc tttaagagta
    28981 aaattagaag atgaaatacc agaaagtgtt attgggattg tcaattctga caagttaatg
    29041 aactatgttt atttaattac tttaaaagaa acttttgatg atgttttaga agttttaaaa
    29101 gaatttaatt tttcagtggt ttcattacca aatgaagaag gtacaccgtt acaagctatc
    29161 aagaaataca atgaaatgat aattagacag gaaaacgttt ctaaggacaa aacagaggaa
    29221 attaaaagac atgcagatag tatacacatg cttgaaaacc tatatgattt ttacactata
    29281 gaaagagacc aaaaaaagat tatagagaga cttgtcaaaa caaaaacaac attctgtctt
    29341 aatggttggt taccaagcaa aagggcagat gaattaatta aagaaattac agaaaagttt
    29401 aattgttgtg ttcagactga agaaggaagt aaagaagaag gcttcccaat tctgcttgaa
    29461 aacaataaat tagtaacacc ttttgaaagt gtaactaaca tgtacagcta tccaagtaca
    29521 aaagatattg acccaaatac aattttaact ttcttctttg ttgtattctt tggaatgatg
    29581 ctaagtgacg ccgcatatgg gttaataatt gcagtagtct gtggatttgt tgtatataaa
    29641 ttaaagatac aaaaaggtga aggaaattta attaaactta ttggtatctg tggagtttca
    29701 actactgtat ggggtcttat attcgggggt attttggggg atttaattcc tataaaagca
    29761 ttaattaatc ctttagagga tgttatgaca cttatgggaa tgtcgttact gttcggtatt
    29821 atccatatat atgtaggttt gggaataaaa gcatatacat taataagaag tggaaaggta
    29881 atagatgcaa tctttgacat aggtttttgg tatttgtgta taacaggtgt atgtctactt
    29941 ataatacctt ttgttgcagg ggatataggt gtattttcag aagtaggaaa atatttagca
    30001 attataggtg ctataggtct tgtacttact caaggaagaa gctttaaagg tattcctgtt
    30061 aagcttttta aaggattttc aagtctctat ggtataacaa gttattttgc tgatatatta
    30121 tcttatacaa gaattatggc gctttgttta acaacaggag ttatagcaca agttatcaac
    30181 cttttaggag ctatagctgg tccgatactt gcagttgtaa ttggtgttgt aggacacaca
    30241 ataaaccttc ttataaatgc actaggtgca tatgttcata caagtaggtt acagtatgta
    30301 gaatttttca ataagtttta tgaaggcgga ggagttcctt ttgttccgtt taagtataaa
    30361 acaaaatata caagcattaa taaaaaggag atgtaaatat ggaattttta aacgcgttag
    30421 gaaatggtca atttttggca atatcaggag ctgctatagc agcattattt gcaggtatgg
    30481 gttctgctaa aggtacttca atagcaggtc aagccgcagc aggtgttgta acagaagacc
    30541 ctagtaaatt cggtcaactt ctagttttac aacttcttcc tggtacacaa ggtctgtatg
    30601 gatttatcat agctttcctt attttatcaa aagcaggaat tattggtgga gatgcaatac
    30661 caacatcagc tcaaggttta caacttctta tggcaggact tccaattggt ttagttggtt
    30721 tagtatcagg tatagcacaa ggtaaagcag cagcagcagg agttggaata gttgcaaaaa
    30781 gacctgaaga acttggtaaa gctataacat ttgcagcaat agttgaaaca tatgctcttt
    30841 taggactact tgtttctttc ttagcttaca atggaattgc aattggttaa ttataaatta
    30901 taattcataa gtaaaaaaaa tgaaaaggag gaagcactcc tatgggtaat gaacaaaaaa
    30961 tgattgacag aattatagct gatgccaaac aagaagcaca agaaatattg gataaagcta
    31021 aaagtgaagc agatttaaaa gtaaattcag ctaatgaaaa agctgaaaag gaaatggctt
    31081 cctatacaaa gcttgcagag gcagaagcag aaaaagcggc ttctaaggaa atatcaggag
    31141 cttatatgga ggctaaaaag caaatattat caaaaaaaca agagatttta gaagaggtaa
    31201 ttttagaagc taaaaacaaa cttttaaatc ttaaagataa tgaatatgaa gagattatac
    31261 taaacatgat tgaaaaatca aattgtacag atgattcaga gatagtatta tctaaaaaag
    31321 ataaaaaaac attaaaagat gtgctatcaa aaaaaggcat taaagtgtct gatgaaacta
    31381 gagatattac tggaggtttc atagttaaaa aaggtgacat tgagtacaat tattcatttg
    31441 aagccataat tgcagtagag catgaatata ttgaacaaat tgcagcagaa attttgttta
    31501 attaaggagg ggttaaaaaa tggcagaaga aaaaacttat ccctatgccg ttgcacgaat
    31561 aagagtgctt gaaaaacagc ttcttaacag acaaatgttt attcaaatgg cagaagccaa
    31621 aagccctgaa gactctttaa gaattatagc tgaggcaggt tatgctgata catcaaataa
    31681 taatatacat aattttgaga atattttgac tcaagaatta tcaaagacat atgaaattct
    31741 taaaagtcta gctcctgaag aaaaatttgt tgatgtattt ctttataaaa atgactatca
    31801 caatttaaaa gtgctgatta aagaagaaat ttctggtgta gatggtgaga aatatttaat
    31861 tgatggaggt acaattcctc ttattaagct tagagaatct ttagcaaatc gtagttttag
    31921 tgatttacct aagataatgt catcagctat attagaggca tttgatgttt ataataagac
    31981 acaaaatggc cagatggttg atattgttct agataaagca acctttacca gtatgaaaga
    32041 aactgctaaa gaaagccaaa acaaatttgt aatagattat gttatgaaag tttgtgattt
    32101 aacaaatcta aagagtttta taagagtcaa aaatatgaag aaagattttg atatgtttat
    32161 gaatgtgttt gtttcaggtg gctcacttga taaggaaaaa ttcttagaag ctttttcctc
    32221 agatactcca gcatcatgtt ttaaatctac ttcttactct gatgtctgta aaaatggcat
    32281 ggatagtggt tttactgtct ttgaaaaact ttgtgatgac tatcttatgg aatatgttag
    32341 aggagcaaaa tttaagcctc ttacattaga accactaatt gcatatttat atgctaaaga
    32401 atcagagata aaaacaatta gaattatttt gactagtaag cttaataata tagacgcaga
    32461 tactataaaa gaaaggctaa gggatgctta tgtataaggt aggagtagtt ggagataaag
    32521 atagcatcat gggattttta gctcttggaa tagatatctt cccagcctat gatagtgacg
    32581 aaattaaaaa aagtatacat aagttggttg aagatgaata tgcgataatt tatattacag
    32641 aacaggcatc attgcttgca aaagaatcaa tagcaaaata caaagattac caacttcctg
    32701 ccattatagt tattccaggt attggaggaa gtatgggatt aggtatgaat gaagtaagag
    32761 aatctagtaa acgtgctata ggtgcagata tattatttaa agaatagatg aaagttgaat
    32821 aaaagacaat ctcaaatttt attggtgcta aatatatgtg ctaataaaat ataggaggga
    32881 atatttaaat tttgataaag tatatgattg caggtgagag aatatgaaaa caggcacaat
    32941 tgtaaaggtg tcaggaccgc ttgttgtagc ggagggcatg agagatgcga atatgtttga
    33001 cgtcgtaaga gtttcagata aacatcttat aggcgagatt atagaaatgc atggagataa
    33061 agcttctata caggtttatg aggaaacttc cggtctagga ccaggggaag aagtagtttc
    33121 tcttggaatg cctatgagtg ttgaacttgg accaggtctt atttcaacta tttatgatgg
    33181 tattcaacgt ccacttgaaa aaatgtatga tatttcaggt actaatataa ctaaaggtgt
    33241 tgaagtatct tcacttgata gaacaactaa gtgggaattt aaacctaaaa aatctgttgg
    33301 tgataaggtt gtagcaggag atataattgg tggcgttcaa gaaacagcag tagtagaatg
    33361 taaaattatg gttccttatg gagttaaggg tactataaaa gaaatatatt ctggtgaatt
    33421 tactgttgaa gatacagttt gtgttattac agatgaaaaa ggtaatgata tacctgtaac
    33481 tatgatgcaa aaatggccag ttagaaaaga aagaccatat agagaaaaga aaacaccaga
    33541 ttcacttctt gttacaggtc aaagagttat agatacattt ttcccaatca caaaaggtgg
    33601 ggtagcagct ataccgggac cttttggaag tggaaaaact gttacacagc atcagcttgc
    33661 taaatgggca gatgcagata tagttgtata tataggctgt ggcgagcgtg gaaatgaaat
    33721 gacagatgtt cttcttgaat ttcctgaatt aaaagaccca agaacaggcg agtcacttat
    33781 gcaaagaact gtgcttatag caaatacatc agatatgccg gttgctgcac gtgaagcttc
    33841 tatatacact ggtattacaa tagctgaata ttttagagat atgggatata gtgttgcact
    33901 tatggcagac tctacatcaa gatgggctga ggctcttaga gagatgagtg gtcgtttaga
    33961 ggagatgcct ggtgaagaag gttatcctgc atacttaggt tcacgtcttg ctcaattcta
    34021 tgagagagca ggaaaggtaa attgtctagg tatggatgaa agagaaggaa cacttacagc
    34081 aattggtgca gtttctcctc ctggtggtga tacatcagaa cctgtcagcc aagcaactct
    34141 tcgtatagta aaagtattct ggggtcttga tgcatcttta gcatataaac gtcatttccc
    34201 agcaattaac tggttgacta gttattcact ctatcaggaa aaagttgatg tatgggcaga
    34261 taaaaatgta aatgataatt tctcagctca aagagctcaa gctatgaaac ttttacaagt
    34321 tgaatctgaa cttcaagaaa tagttcaatt ggttggggtt gattcacttt cagatggaga
    34381 tagattgata ctagaggtta ctcgttctat aagagaagac ttccttcatc aaaactcttt
    34441 ccatgaagtt gatacttata catcacttca taaacaatat agaatgatgg gacttatatt
    34501 aaaattctat aagtcagcac aagatgctat caagcaaaat gttactataa acgatattat
    34561 caagctttca gttcttgaaa agattggtcg tgctaagtat gtagaagaag gcgatataga
    34621 tgcagcttac gattcaattg aagatgaaat agacaacgag cttgcagacc ttattaaaaa
    34681 gaggggggct gaagaactat gataaaggaa tataagtcaa tacaagaggt agccggtcct
    34741 cttatgttaa taaacggcgt agaaggtgta aaatttgatg aacttggaga aattgaactt
    34801 tcaaatggag aaattcgccg ttgcaaagtt ctagaggtaa atggagatac tgcacttgtt
    34861 cagcttttcg aaagctcaac aggtataaac cttgctgaaa gtaaagttcg ttttttagga
    34921 aagagtcttg actttggagt ttcaccagat atattaggtc gtgtttttag tggaatgggt
    34981 agacctattg atggaggtcc agaaattata ccagataaaa gacttgatat aaatggagct
    35041 ccaataaatc cagcagcacg tgattatccg gccgagttta tacaaactgg agtctctgct
    35101 atagatggac ttaatacatt ggttagaggg caaaaacttc ctatattctc agggtctggt
    35161 ttgccacatg ccaatcttgc agttcagatt gcaagacagg caaaagtacg tggaactgat
    35221 tctaagttcg ccgttgtatt tgctgcagtt ggtataactt ttgaagatgc tgaatttttt
    35281 ataagcgatt ttaaagcaac tggtgcaatc gaccgttctg tagtgtttgt aaaccttgca
    35341 aatgacccag ctgtagagcg tgtatctaca cctagaatgg cacttacagc agcagaatac
    35401 cttgctttcg agcaagatat gcatgtactt gttataataa cggatattac aaactatgca
    35461 gaggcgttgc gtgaagtttc agcagctaaa aaagaagttc caggacgtcg tggatatcca
    35521 ggatatcttt atactgacct tgctactatg tatgaaagag caggaaaaat gaaagggcat
    35581 gaagggtcta taactatgat tccaatactt acaatgcctg aggatgataa gacacatcca
    35641 attcctgacc ttacaggata cattacagaa gggcaaatta tacttagccg tgaactttat
    35701 aaaaaagata tacagcctcc tatagatgtt ttaccatcac tttctcgtct aaaagataaa
    35761 ggtattggta aaggtaagac tcgtgaagac catgcagata caatgaacca gctttttgca
    35821 gcttattcaa gaggtaagga tgcaaaagaa cttatggcta tacttggtga atctgcactt
    35881 tctgagattg atttaatgta tgctaagttt gcagatgaat ttgagaaaga atatgtttca
    35941 caaggattta gaactgatag aactattgag cagacattag aaatcggatg gaagcttctt
    36001 agtatgttac caaaaagtga acttaaacgt atccgtgatg aatattttga aaaatatatg
    36061 cctaaggagt gatatctgtg gctagattaa atataaatcc aacacgtatg gaaatgacta
    36121 gacttaagaa actccttaaa actgctacaa gaggtcataa gcttcttaaa gataaacttg
    36181 atgaacttat gaaacaattt cttgagattg taagagaaaa taagcgattg cgtgaagaag
    36241 cagaaaatgc tcttgatact gcttataaaa actttataat cgcaagagca gttatgagtc
    36301 aggagtatct gggttcagca cttatgatgc caaagcaatc agtttcagta gatgtttcta
    36361 cgagaaatat aatgagtgtt gatgttcctg tgtttgactt taaaactgaa aacaatcaaa
    36421 gtgatattta tccttatggt cttgctttta catcaggtga attagattct gctatggaag
    36481 ctttctcaga tgctatgcaa ccacttttaa gacttgcaga aagtgaaaaa agtgcacagc
    36541 ttcttgcaca agagatagaa aagacacgta gacgtgttaa tgctcttgaa aatgtaatga
    36601 taccaaatta cattgagact attaagtata tagctatgaa gcttgaagaa aatgagagag
    36661 caagtactac tagacttatg aaggttaagg atatggtgct taaaaaagca cttgaagaaa
    36721 agaaaaaaaa tgatttggtt gtataattaa atacttataa aaataagtct aacattctac
    36781 tttatagagg ttagacttat tttttatgtt taaattaaaa tgtagatact ataaaaatta
    36841 atatattgtt taaaaacaga ctgtatttaa ctaaaaataa tatatttaac aaaaatttct
    36901 aaaaattaac gaactgttaa tttacactta actttatatt aacaaattag gtgtattata
    36961 taaacataag caaaaacaat aaaaaacaaa taaaaaaagg aaaaaacaca aagggggaag
    37021 atatgaaaga atttatattt acttgtctgt gcattgtagt gattacattt atgacaagtt
    37081 gcatatcaac aacaccacaa actactcaag gaaaaggaaa taataaactt acaatatatg
    37141 cagcactaga ggaagagcag atagcttatt atatggaagg ttttagagaa gagtatccca
    37201 atatagatgt agagatagtt atggattctc atggtgtaat agcttcaaaa ctattagcag
    37261 aaaaagataa tccacaagca gatattatat ggggattatc agcaatgaac atgatagagc
    37321 taaaaaaaga tggtgcactt gaagcatata agcctaaaaa ttatgataaa gttgataaaa
    37381 aatttaaaga tactgaagat gatgtatctt gggtaggaat gagtgtccca gagacagcaa
    37441 tagtagttaa ttataaggaa cttaagaaac taggtataga tattccaaaa agttatgaag
    37501 atttaataaa accagaatat aaaggactta taacaatgcc aaatccagca tcatctggaa
    37561 cggggtatct tacagtatca gggcttattc aaatgatggg tgaaaaagat gcttacaatt
    37621 acatggataa attacataaa aatatagcca catatacaca ctcgggttca aaacctgcga
    37681 agttagctgg ctctggagag tatccaatag gtattacact tggatataga gctacacaac
    37741 aattatctag tggagaacct atagaggttg tatttccaaa ggaaggttca ggctgggact
    37801 tagaagcaaa tgccttagtt aaaaaagata atataaaaaa agaagcaaag ctatttttag
    37861 attgggcaat aagtgattca gctatggata gatatgcaca tgaagtagca gttacttcca
    37921 taaagacaga taatccagtt ccaaaaggtt attctaaaca accatttgaa cagcttataa
    37981 aagaagttga tttaaactct gctgccaaaa acagaagtac agtacttaaa acttgggaat
    38041 caaaatacgg ttcaaaatct gaaagtaagt aaagtttaaa ttaataattt agatgaaaga
    38101 attttagaag aaaaagtagg atataaaatt taaaataaaa aaataactat ataaggagag
    38161 ataagatatg agttacttaa aaataaataa tgtgtttaaa agttatgacc aaaaaagggt
    38221 tttaaataat attagtttgg atattgaaga aggagaattt ctatgtttac tcggacctag
    38281 tggatgtggt aaaactacat tacttagaat aatagcaggt cttgaagatg taaatagcgg
    38341 aactataatt ctccaggata aagatattac aaatttagag ccatcaaaga gaggttttgg
    38401 tatagtattt caatcatatg cattatttcc gaatatgact gcttataaca atatagcttt
    38461 tccactaaaa gagagaaaag tttcaaaagg aaaaatagat aataaagtta aggaagtact
    38521 tgaaacagtt ggattgacta atgaagctca taaatatcct aaagcattat caggtgggca
    38581 acaacagagg attgcaatag caagagcatt agctcttgaa ccaaagtttt tacttttaga
    38641 tgaaccaatg tccgcattag atgcaaaggt tagacacaag cttagaatgg acattaaaag
    38701 attacaaaaa gaacttaata taacgacaat aatggtaaca catgaccaag aagaagctat
    38761 aactatggca gataaaatag ctattctaaa tggaggagat ataatgcaaa tagggactcc
    38821 agaagaaata taccaaaatc ctcaaaacct atttacagct caatttatag gagatacaaa
    38881 ctgttttgat aatggagatt ctatattaac tgtaagacca gaatatgttc aaatagagaa
    38941 atcgacaaaa gaaaactacc aaggaataat atcaaatata gagtttagag ggaatttatt
    39001 gagagttgaa ataaaagata aattaaatga aaatttcata ataagtgatg cttctataaa
    39061 agagtgggtt aatttaaatt tagtagaagg tgaccttgta aaaataagta tagacgaaaa
    39121 atactattta aagtatccaa aagttaaagg agcataagtg tatgaataat aatgctataa
    39181 gttctaaaag tataagtctt aaatcaaaaa tgaatttaga tataaaaaaa tgtatgctta
    39241 cagtattagt tatgtcttta gtaatatttt tattagcacc tataatatat ctatttgtac
    39301 aggctttttt agataaagat ggagtctttg taggattaca aaactttcaa gaatacttgg
    39361 cttctgaatc tctattaaat tctctaaaaa atacagtatt tgtatcagtt atatcaactg
    39421 taatatcact aacacttgca ttagtatatg catatgcatt aactagaaca aagattaaat
    39481 ttaaaggttt ttttaaatca ttagctatgt ttcctatgtt tgctccaaca atgatgcatg
    39541 caataggtct agtatattta tttggaaatc aaggtttggt aactaattta cttggagtgc
    39601 atgttccaat atatggagca gtgggaataa tattggcaca aattatatat gtatttcctc
    39661 aatcatttca aatactttat atatctctaa gttcaaccga ttatagacta tatgaagcta
    39721 gtaatactat ggggataagc aagataagag aatttataag cataacaata ccaagtataa
    39781 aatactcttt aataagttct gcattcgtat cgtttacatt atgttttaca gattttggag
    39841 ctccaaaggt gataggagga gagtttaatg tattatcaat agatgtatat aagcaaatta
    39901 tagggcaaca gaatatgcca atgggagcaa ctgtatcaat attactttta atacctgcaa
    39961 taatatcgtt tatagtaact aaaaaaattg aaaaaaatca aaattcatat ataacttcaa
    40021 agtcaattcc atataaggtt aaaactaata agttaagaga tagtatttta ggaggggttg
    40081 taggaattat tgcatttggt ataatagctg taatgttaac tgtaatctta tcttcattaa
    40141 tatcagtttg gccatatgat ttaagactga cactggaaca tttttctatg aaaaacagta
    40201 ttgatgggat taaaccattt ataaattcaa taaaaatgtc actattgaca gctattctag
    40261 ggacgagctt tgttttctta tttgcatatt tgaatgaaaa aacagaaaaa gctccatatt
    40321 taaaatcatt tgggtatttt ttaagtacat tgcctatggc attgccagga ttagttttag
    40381 gtatagctta tgtattattt tttaataaat tagagtttaa ttttatggat acaatataca
    40441 ttaagaattt gtttaatgga ttatatggaa cattacttat aatggttata tgtaatatag
    40501 ttcatttctt ttcagtagca tttataacag ctactactgc tattaagaaa gtagataaag
    40561 aattggatat ggtagctaaa tcaatgggaa taaacagcgc atcaattctt aagaacgtaa
    40621 ctataccact ttgtttgcca gcagttttag agaactttat gtactttttc ctaaactcca
    40681 tggtgacaat atcagcatta gtttttatat atactgcaag ttcaaaagta gctgcaatat
    40741 caattgtaca actagatgat aaggggagta ctgcacaggc agcatcacta gctgtgttaa
    40801 tattagtcac aaatatatta gtgaaagtaa tatatgaatt tataaagaat cgtttagaaa
    40861 aaagaacaaa ctttaattaa ataatttatc ttataaaaac acaaatttaa aaaagtctat
    40921 tcttgtgatt tattatttaa agaatagact ttttaaattt attattgata ttgtttatta
    40981 attttccttt tgttatcata catttgagaa tctgcacgac gatatgtatc aaataaagaa
    41041 acatctactt tttcatcaaa aatagcatat ccaatagaca ttgataattt taattcatta
    41101 gttgagtt
//
LOCUS       DAGSRM010000029        40163 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.029, whole genome shotgun sequence.
ACCESSION   DAGSRM010000029 DAGSRM010000000
VERSION     DAGSRM010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40163
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.029"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(441..2828)
                     /gene="cadA"
                     /locus_tag="MJE33_001649"
     CDS             complement(441..2828)
                     /gene="cadA"
                     /locus_tag="MJE33_001649"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBY9647589.1"
                     /translation="MEANNSIKKEFILGGLNCAHCAEEINNKVSKLQEVKSSNLNFIN
                     KKLTVNIKESFNEDTTIEKIIDIIDSTEPGLDIQISSKENAASKTSIKKELILGGLNC
                     AHCAEEINNKVSKLKEVESSNLNFVNKKLTVNISNNFEEDDVINKIKEIINSTEPGLD
                     IQVGSTDKVKGRTTEKSGAVNDTNKKELIPLIIGALVYIFGIYQTATGYESQFSNIVF
                     IVAYVIVGGDVLLRAIRNISKGRVFDENFLMALATVGALTIGELSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKVNSEVKVVSPEEVNVGDIIVVKPGEKVPLD
                     GVVVDGVSMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFVPPLVISGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDIVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGNFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKVYRGYIVISDEVKEDSK
                     NAIRSLKEIGVKEVVMLTGDNEKVAKNIAQELELDTVYSNLLPNEKVDRLEDLYEGRT
                     EKEKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKIIVMILGAGGVATMWEAIFADVGVALIAVVNAMRAMR"
     gene            complement(2851..3216)
                     /locus_tag="MJE33_001650"
     CDS             complement(2851..3216)
                     /locus_tag="MJE33_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY9647590.1"
                     /translation="MREEINDCNCNIVHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            3415..4263
                     /locus_tag="MJE33_001651"
     CDS             3415..4263
                     /locus_tag="MJE33_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647591.1"
                     /translation="MSNKKKKDLDTSYMPYLDPANYADYITNEEIPQDELRNDPQISN
                     GYTKIPNNQNINNLYPNNVNHYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNN
                     NTGMPNNMSNNMNSNMGMPNNMPNNMNSNMGMPNNMPNNMNSNMGMPNNVPNNMNSNM
                     GMPNNMSNNMNSNMGMPNNMSGNMNNNMYGNTGMPNNMNSNMGYNMNNTMGIPNFSCN
                     QNMPPNVLMMIPGVICHNTMQGMPVVMPSTMPPNIYPTPYGSSNMSVQGIPQATNIEE
                     FDEEEM"
     gene            complement(4700..5788)
                     /locus_tag="MJE33_001652"
     CDS             complement(4700..5788)
                     /locus_tag="MJE33_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647592.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLTIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKEPMYDFEVFDEISKSEFVSVYSVNSPEEFAGVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENVYIINNDMKAIYYLMGNKFFIGTYNENDCNYINEISQSNYSDYIK
                     FEEKFFFEQNALYDFAESGSLDFDDFLE"
     gene            5913..6641
                     /locus_tag="MJE33_001653"
     CDS             5913..6641
                     /locus_tag="MJE33_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBY9647593.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
     gene            complement(6843..7334)
                     /locus_tag="MJE33_001654"
     CDS             complement(6843..7334)
                     /locus_tag="MJE33_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647594.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFKSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIEYFM
                     KLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVYSSFIGYYNNRLRKML
                     FKK"
     gene            complement(7345..7788)
                     /locus_tag="MJE33_001655"
     CDS             complement(7345..7788)
                     /locus_tag="MJE33_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647595.1"
                     /translation="MYSNTYNNENFMRARSFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            complement(7778..8617)
                     /locus_tag="MJE33_001656"
     CDS             complement(7778..8617)
                     /locus_tag="MJE33_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647596.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKNKLKLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFVYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGK
                     NVQ"
     gene            complement(8836..10221)
                     /locus_tag="MJE33_001657"
     CDS             complement(8836..10221)
                     /locus_tag="MJE33_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBY9647597.1"
                     /translation="MDNLNKKFLMEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KECLKKHGFSIEGLEGMDTAFVASYGEGRPVIAVLGEYDALDGLSQKVSTTKEPIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDNCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMEHVRDVFGRIVDIA
                     KGAALMTGTIMEYDIISGVYDYIPNTILTDILSQNMKLVGVQNYNEDDYYFADKLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELKD
                     TVGSFKYIPII"
     gene            complement(10394..11137)
                     /locus_tag="MJE33_001658"
     CDS             complement(10394..11137)
                     /locus_tag="MJE33_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HBY9647598.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IDSEHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            complement(11157..12419)
                     /locus_tag="MJE33_001659"
     CDS             complement(11157..12419)
                     /locus_tag="MJE33_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBY9647599.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYRATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIDGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQDIKELEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMTRANGDLWLKDNKPNINIYQSG
                     LGFEMDKDSDFVVNAHKILEETLGKKLEIKGSTAGNDARVMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            complement(12603..13601)
                     /locus_tag="MJE33_001660"
     CDS             complement(12603..13601)
                     /locus_tag="MJE33_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBY9647600.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSVFRDQGSAAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWVGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKSAKSK"
     gene            complement(13585..15096)
                     /locus_tag="MJE33_001661"
     CDS             complement(13585..15096)
                     /locus_tag="MJE33_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBY9647601.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDVSSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVENLTKNNVFNDISFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKEILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            complement(15150..16112)
                     /locus_tag="MJE33_001662"
     CDS             complement(15150..16112)
                     /locus_tag="MJE33_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBY9647602.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            complement(16160..17050)
                     /gene="rbsK"
                     /locus_tag="MJE33_001663"
     CDS             complement(16160..17050)
                     /gene="rbsK"
                     /locus_tag="MJE33_001663"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBY9647603.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGEDGLGEELIRNFRTNKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRIRVL
                     LNPAPAVKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLVITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKNLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            complement(17092..18105)
                     /locus_tag="MJE33_001664"
     CDS             complement(17092..18105)
                     /locus_tag="MJE33_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBY9647604.1"
                     /translation="MSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKIMKE
                     KNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNTDED
                     AKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETLKGI
                     VKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYNPAY
                     VFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDISVVGFD
                     DIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRDSTK
                     ESE"
     gene            complement(18334..19311)
                     /gene="bioB"
                     /locus_tag="MJE33_001665"
     CDS             complement(18334..19311)
                     /gene="bioB"
                     /locus_tag="MJE33_001665"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HBY9647605.1"
                     /translation="MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSYYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMAFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIEQGFEF"
     gene            complement(19517..19780)
                     /locus_tag="MJE33_001666"
     CDS             complement(19517..19780)
                     /locus_tag="MJE33_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647606.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQAMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
     gene            complement(19850..20923)
                     /locus_tag="MJE33_001667"
     CDS             complement(19850..20923)
                     /locus_tag="MJE33_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBY9647607.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDEAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
     gene            complement(21182..21940)
                     /locus_tag="MJE33_001668"
     CDS             complement(21182..21940)
                     /locus_tag="MJE33_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9647608.1"
                     /translation="MREYIAFTKKEFKENLRNYKLFSLIILFLIFGISSPISAKFMPD
                     LIAHFAPTLKVTAAPTALDSWTQFFSNISGLGMSLTLIIFCNILSNEYSKGTLVIMLT
                     KGLSRSSIVLSKFSVTVIIMTIGFWLSFLFAYGYTMYFWPTANLNHIIFSAFNLWLIG
                     IMYISVLILGCVLFRPAFASVLLVLVATLLLSLISIPKQIAPYTPNFLMSKNIDLISG
                     KVAMPEFIIPIIITVLISIVCLLAAVILFNKKPV"
     gene            complement(21937..22854)
                     /locus_tag="MJE33_001669"
     CDS             complement(21937..22854)
                     /locus_tag="MJE33_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9647609.1"
                     /translation="MNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKTT
                     TMKMVLGLLKPDGGTIDVCGEKVTYGKTSSNRHVGYLPDVPEFYNYMRPLEYLSLCGE
                     ITGLSKKEIQIRSEELLSLVGLGNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDEP
                     TSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLSE
                     IKSMHGKDRLLLEFASNDEIQKFKSSDGIKSLLKDSEETNMEIVLHGKDIKAIQKTVI
                     STLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            complement(22854..23054)
                     /locus_tag="MJE33_001670"
     CDS             complement(22854..23054)
                     /locus_tag="MJE33_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc_N domain-containing protein"
                     /protein_id="HBY9647610.1"
                     /translation="MENLMEYLPLLVPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            complement(23059..24153)
                     /locus_tag="MJE33_001671"
     CDS             complement(23059..24153)
                     /locus_tag="MJE33_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY9647611.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     PDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTVKPFDEVMDEIREI
                     IKKYYSCFPLIFRMGLLIVNHYDIVDEKKRELLISEALEIFIRIQETCNDIDICRQAK
                     SMEATCYILLNQPIQVIDLLQNSNFPMINESILLAQGQMMNGQMDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISDIFELDTLSPAIMLSSYLTQAQINLIH
                     GDNEGAIKSLRKYVDLATRDIYPIIIHGDDFFNKLDRWISEIGTGITRDDTIVKAGIV
                     AAIKNNPMFSVLSENKEYKFLIEKLSLLEE"
     gene            complement(24663..24803)
                     /locus_tag="MJE33_001672"
     CDS             complement(24663..24803)
                     /locus_tag="MJE33_001672"
                     /inference="COORDINATES: protein motif:HMM:NF021468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HBY9647612.1"
                     /translation="MNGEGCILSNKITKECYKVGYMIREKSIDIYPDSGWGFMTWDEN
                     GE"
     gene            complement(25113..26420)
                     /locus_tag="MJE33_001673"
     CDS             complement(25113..26420)
                     /locus_tag="MJE33_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBY9647613.1"
                     /translation="MDISRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEIGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETGMKCIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYIIVSGGTKENVVPSHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIVLNNIIDRFKK
                     VFCNGWKIEVIAHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            complement(26485..26964)
                     /locus_tag="MJE33_001674"
     CDS             complement(26485..26964)
                     /locus_tag="MJE33_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HBY9647614.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGANPDDIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQGVKLDFAQKCAHDDEVCELVFTKK"
     gene            complement(27011..27874)
                     /locus_tag="MJE33_001675"
     CDS             complement(27011..27874)
                     /locus_tag="MJE33_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBY9647615.1"
                     /translation="MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKRKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            complement(27874..28629)
                     /locus_tag="MJE33_001676"
     CDS             complement(27874..28629)
                     /locus_tag="MJE33_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBY9647616.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            complement(28656..29144)
                     /locus_tag="MJE33_001677"
     CDS             complement(28656..29144)
                     /locus_tag="MJE33_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY9647617.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            complement(29162..29563)
                     /locus_tag="MJE33_001678"
     CDS             complement(29162..29563)
                     /locus_tag="MJE33_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HBY9647618.1"
                     /translation="MNKYKVIILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            complement(29566..30063)
                     /locus_tag="MJE33_001679"
     CDS             complement(29566..30063)
                     /locus_tag="MJE33_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY9647619.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNQNINRY
                     KGEFI"
     gene            complement(30087..30491)
                     /locus_tag="MJE33_001680"
     CDS             complement(30087..30491)
                     /locus_tag="MJE33_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY9647620.1"
                     /translation="MIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNIEDL
                     NKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLEALI
                     NREGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            complement(30721..33288)
                     /locus_tag="MJE33_001681"
     CDS             complement(30721..33288)
                     /locus_tag="MJE33_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY9647621.1"
                     /translation="MTQKKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFDNINSINEYS
                     ENDSIKDEYILESIKKENNIFNLVGCDGSLRSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLTIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGVLFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRIPVTIKIPSLEERGFQERMKLICYFFKEESVRIGVKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKDFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDGDIQKYSYNLMNTFIYNSSNQTAYNNIVNDSIATTVKYILQKHDKWSK
                     REGLDKLIKAIALHIQNLIERIKIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMY
                     GVEFPEDEAIFLATFLYLSYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVA
                     INMGLEDRVQDILVQAVEIANKIDKGKGILILTDMGSILTFAKVIRDATGKEVKAIDM
                     VSTPIVIEATRKALTPEMTLEKLYFNIIESIKKHYGDMEVTYAEKDNGGRYFDRLLID
                     NISKTLTFLNGEKAYFILKEVINRISEHYSLVIQDELLVKFIFHCSCMIERVIIKESL
                     VYKSYEERISRNKELYLVIKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
     gene            33454..34197
                     /locus_tag="MJE33_001682"
     CDS             33454..34197
                     /locus_tag="MJE33_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBY9647622.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHKWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNSNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DELIMNYPKKLINHFHQKGKLLDVDYTNI"
     gene            35035..35766
                     /locus_tag="MJE33_001683"
     CDS             35035..35766
                     /locus_tag="MJE33_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647623.1"
                     /translation="MLSKKNVSLIILVIILSLLTAIFIFNNKNTNNKLKEVTKNNEEI
                     VPKNLEGLSIEQLIEIRDKAIDKNLEGLREFGEDSVKKRKDSILNFEFGLVAFVNKNY
                     SNLTEKEKLIRQINIVNYENSINDIMDPSGSFFELANQNYQVNEFLLKNGKDYKEWSY
                     EGLDFLIDHLEELCKFVDFDVKDVIDIIDPTIKTDLSEEQKKSLNDKLKKISSSDTLS
                     EEVKKEIKNWENNLNNLNMNKNNSN"
     gene            complement(35923..36129)
                     /locus_tag="MJE33_001684"
     CDS             complement(35923..36129)
                     /locus_tag="MJE33_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647624.1"
                     /translation="MSKYSSLWEYVKKNNSQSFKLTFEEIKDIAGIEIDHSFLKYKKE
                     LNEYGYQVGKISLKEKTVIFDKIG"
     gene            complement(36532..36927)
                     /locus_tag="MJE33_001685"
     CDS             complement(36532..36927)
                     /locus_tag="MJE33_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBY9647625.1"
                     /translation="MKALEFLNKARVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
     gene            37068..37460
                     /locus_tag="MJE33_001686"
     CDS             37068..37460
                     /locus_tag="MJE33_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY9647626.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESTGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNTCGLSCKECRKIK"
     gene            complement(37560..37976)
                     /locus_tag="MJE33_001687"
     CDS             complement(37560..37976)
                     /locus_tag="MJE33_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBY9647627.1"
                     /translation="MNWQDAFFQDTKPKFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVSESNRVEVDLFINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(37991..38902)
                     /locus_tag="MJE33_001688"
     CDS             complement(37991..38902)
                     /locus_tag="MJE33_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBY9647628.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYSTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTTD
                     FAIEMYYPTTPEAAYLEHWIVPVPIEQ"
     gene            complement(39084..40106)
                     /gene="splB"
                     /locus_tag="MJE33_001689"
     CDS             complement(39084..40106)
                     /gene="splB"
                     /locus_tag="MJE33_001689"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HBY9647629.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKKIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLARAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
BASE COUNT        13217 a         7171 c         4161 g        15614 t
ORIGIN      
        1 cttaaaaata attcgtcaat actttttaaa attatttttt aaaaatattt actttcttat
       61 attacataat ttattgaatg gcagtcatac tgttggttta gagtatttta acacacacat
      121 tttgttgtaa aaattacaac tattaatata atatatatac aatcataagt attataattt
      181 ttatgaatct aatttaaact taatataaac ttattaataa accttctatg tctaaatttt
      241 aaaattacaa tataaaagct ctacactaaa taaatacatt aaataaacat aatggataat
      301 gaatataaat ttatatatgc aaagcgctcc aataaaaagg taccaataaa gttaaaagaa
      361 acttattgat acctttcatt attacatact tcaatatata attataagtt ttttaaatag
      421 tatggattta catttatccc ttatctcata gctctcatag catttactac tgctataagt
      481 gccacaccta catcagcaaa tatagcttcc cacatagtag ctactcctcc agcacctagt
      541 atcataacta taatttttac tcctagagca aatattatgt tttgccacac tatcttatta
      601 gtcttgtttg ctatttcaat tgctttagat atcttgctag gctcatctgt cataagcact
      661 acatctgcag cttcaatagc agcatctgaa cctaagcctc ccatagctat accaacatca
      721 gcacgagcta atactggagc atcatttatt ccatcaccta caaacgctat tttctctttt
      781 tcagttcttc cttcatataa atcttctaat ctatctacct tttcattagg aagtaaattt
      841 gagtaaactg tatccaattc taattcttga gcaatatttt ttgctacttt ctcattatct
      901 ccagttaaca ttacaacttc ttttacccct atttctttta agcttcttat ggcattttta
      961 ctatcttctt ttacttcatc agagataact atataacctc tatatacttt atcaacagca
     1021 atatagacta ctgtgcctac ttctttagct gatgaataag atatattttc ttttttcata
     1081 agtttctcat tacctgctaa tataaaattg ccattatgct ttactctaat tccataagct
     1141 gctatttcct cataactgtc tattgtatct aaatcaattt ttttattata gtaacttaat
     1201 atggactttg ctattggatg atttgaattt acctccgcaa tagctgcata ttctaataat
     1261 tcttcttctg atataccttc aggatttaac ttagttacat tgaacacacc tttagtaagt
     1321 gttcctgttt tatcaaatac tactatatct acacttctta aagcttctaa atagttactt
     1381 cctttaataa ggataccatt tttagatgca aacccaattc cactaaaaaa actaagtggt
     1441 attgataaaa ctaatgcaca agggcaagaa acgactaaga atatcaatcc tctatataac
     1501 caatcactaa atacctcacc tgagattaca agtggtggca caaatgctat taataaagca
     1561 gctattacta ctattggagt atagtatctt gaaaacttag atataaagtt ttctgtcttt
     1621 gattttttaa tgctagaatt ttcaacaagg tcaagaatct tagaaactgt tgattctcca
     1681 aaactctttg taacttcaat actcaataaa gcatttttat ttataacacc tgataagatt
     1741 tcatctcctt tttcaacttc tctcagcact gactctcctg tgagtgcaga agtatctaac
     1801 atagaaactc catctactac tacaccatct aatggtactt tttcaccagg ttttactact
     1861 attatatcac ctacatttac ctcttctggt gacaccacct taacttcaga attaactttt
     1921 agatttgcat agtctggtct tatttgcata agtgaagtta tagactttct ggatttacca
     1981 actgcaacac cttgtaaata ctctcctact ttatagaaaa gcataacccc tactgcttct
     2041 gataactccc ctattgttaa tgctcctact gttgctagag ccatcaaaaa gttctcatca
     2101 aatactcttc cttttgatat atttcttatt gctcttaaaa gcacatcacc acctactatt
     2161 acataggcaa ctataaatac tatattgcta aactgacttt cataacctgt agctgtttga
     2221 tatataccaa atatatatac aagtgctcca attataagtg gaattaattc tttcttattt
     2281 gtatcattta cagctcctga tttttcagta gttctccctt ttactttgtc agtagaccct
     2341 acttgtatat caagccctgg ctctgttgaa tttattattt cttttatttt atttataaca
     2401 tcatcctctt caaagttatt acttatattt actgtaagtt ttttatttac aaagtttaaa
     2461 ttagaagatt caacttcttt taatttagaa actttgttat taatctcttc tgcacaatga
     2521 gcacagttta atcctcctaa tataagctct tttttaattg atgttttact agctgcattt
     2581 tctttagaac ttatttgtat atcaagacct ggctctgttg aatctattat atctattatc
     2641 ttttctatag tagtatcctc attaaaactt tcctttatat ttactgtaag ttttttattt
     2701 ataaaattta aattggaaga tttaacttct tgtagtttag aaactttatt attaatctcc
     2761 tctgcacagt gagcacaatt taatcctcct agtataaatt cttttttaat tgaattattt
     2821 gcttccatat atattcactc ctctcattta tcatttataa gtctctctaa tatgatttaa
     2881 tccacaatca aatatctgac ttatatgact atcatctaat gagtaataca ctgtttttcc
     2941 ttctcttcta aactttacta atctagcctg ctttaatact ctaagttgat gagatattgc
     3001 agaatgagtc atatttaata aactcgctat atcacataca cacatttcat ttgcaaataa
     3061 tgcatataat atctttactc tagtagtatc cccaaatact ttaaataatt ctgctaaatc
     3121 atataacatt tcttcatctg gcatagttga tttagcctct gttactattt cttcatgtac
     3181 tatattacaa ttacaatcat ttatctcttc tctcattttt aatcctccat tattttaata
     3241 tatgaataac tgttcatatg ttcattatat gttatcattt ctaaatttgc aacaataatt
     3301 tttattaatt taatataaat agaatgcaca tattataaac ttttttaata tatatattat
     3361 tagaataagt ttttgtaaac ttttttatag ttttctagga ggtgcaagtt taatatgagt
     3421 aacaagaaaa aaaaggattt agatactagt tacatgcctt acttagatcc agcgaactat
     3481 gcagattata ttactaatga agaaattccc caagatgaat tgagaaatga ccctcaaatt
     3541 tctaatggtt atacaaaaat acctaataac cagaacataa ataacctata tcctaataat
     3601 gtaaatcatt atcctaaaag tagtaattct ccaaacaact tgcctaacac aaataataca
     3661 tccggaaata tgaattacaa tacatctgct acaaataata tgcctaataa catgaataat
     3721 aatacgggta tgcctaataa tatgtctaac aatatgaata gtaatatggg tatgcctaac
     3781 aatatgccta ataacatgaa tagtaatatg ggtatgccta acaatatgcc taataatatg
     3841 aatagtaaca tgggcatgcc taacaatgtg cctaataaca tgaatagtaa tatgggtatg
     3901 cctaacaata tgtctaataa tatgaatagt aatatgggca tgcctaacaa tatgtctggc
     3961 aatatgaata acaatatgta tggtaataca ggtatgccta ataacatgaa cagtaatatg
     4021 gggtataata tgaacaatac tatgggtatt cctaacttct catgtaatca aaatatgcct
     4081 cctaatgtac ttatgatgat accaggagtt atatgtcata atacaatgca aggtatgcct
     4141 gtagttatgc ctagtactat gcctcctaat atatatccta ctccttatgg ttctagtaat
     4201 atgtctgtac aaggtatacc acaagctact aatattgagg aatttgatga ggaagaaatg
     4261 taatatatat ttttttaagt aagttgtata aaacattaat aattgaccat aataataaat
     4321 gtattcctca taatattccc ccaatattat tataggtata gaaagttgac tgaatatgcg
     4381 cttattcggt caacagatat taacataatc taataagata aacatagaaa aagtcagata
     4441 aaatttaatt ttatctgact ttttcattta aaaatgctct cttgtgttgg tgattcagaa
     4501 tagtatggta ctcgtatttg agactaatat tggcaaacaa cataaagaaa aatataaatt
     4561 ttttataata aactgtataa aattttaata attgaccaca ataataaatg tattcctcat
     4621 aatattcccc caatattata gaaataaatt taagcgagat taaattcagt taatctcgct
     4681 taaataatgt ctaaatttat tattccaaaa aatcatcaaa atctaatgaa cctgattctg
     4741 caaaatcata taatgcattt tgctcaaaga aaaatttctc ttcaaattta atatagtcgc
     4801 tataattaga ttgacttatt tcattgatat aattgcaatc attttcgtta taagtaccaa
     4861 taaaaaactt atttcccatt aagtaatata tagctttcat atcattattt attatatata
     4921 cattttcttt tacatggtca ttattaaaat taaatctagt aaagaatata ccttcattca
     4981 tattactttt taacagaaat ggattttctc tataaaatac tcctgcaaat tcttcaggtg
     5041 aatttacact gtaaacactt acaaattcac ttttagaaat ctcatcaaat acctcaaaat
     5101 catacatagg ttctttatct gtcactaata gagaattaat tctaaaacca gtttcataac
     5161 acttacttat gcaaaaagca attttacata tataatatcc atcattatct tcatataaag
     5221 cttcacttat atatctgcct tgacctttat cacttaccac gtttttaagt aatgttccat
     5281 ttatattagt aatgtgcata tttgcaatct cgtcacttcc agaaaagtac tttaaacttt
     5341 ctctatccca tgctataaat ctcatggtca tatagtttat aaattcaact tcctcgtcaa
     5401 ctcttttaca tattttattg tataattttt ctatagttaa atctgtttta tagtttttta
     5461 taatatcaat atactcacat ttatctcctg gcaatagctt atcataataa agattcttat
     5521 taccaaaata agatactaaa aataaagatt catccttcgt taattcaact ctatctgaac
     5581 caaatcctcc catcaatctt tcttcttcca tataagcttc ttcttgtgtt gggttattta
     5641 aactcacata atccgctaat cctaatccct ctgcatctaa taaaaaatat tgacaaaagt
     5701 gattgttttc atcatctatc caatttatta taagacctaa actgcccata agtctagaat
     5761 ttgtagcctt tgcaaaatta aaattcatat agtcacatcc tttaaactct aaaatacttg
     5821 ttttttaata tataaattat tatacatata ttttaacaac ttagatacaa tttataatat
     5881 aatataagga aatgattaat tgaggtggtc ttatgaaaaa tatagcagct ttttttgata
     5941 tagatggtac tctttataga gactctctta tggttgaaca ttttaaaaaa ttaattaaat
     6001 acgatataat tgaccaaaaa aattggtata aacatgctag agacactttt atggattggg
     6061 ataaaagaca aggtaattat gatgattatc tattagaaat atgtgacctt tatgttgatt
     6121 cactaaaagg attagataaa acctgtatca actttacaag tgaccaagta attaaattaa
     6181 aagcagatag agtttataaa tacacacgtt caaggattga gtggcatcta aatcaaggac
     6241 acatagttat atttatatct ggtagccctg gatttcttgt tgaaaaaatg gcaaaaaaat
     6301 ataatgtaac cgattttcta ggaagtgatt atgtctttga aaacaatata tttacaggca
     6361 cagttattcc aatgtgggat tctataagta agaataatgc tataaacgat tttgttgtaa
     6421 agtatgattt agatttatca aactcgtatg cctatggaga tactaatggt gatataaata
     6481 tgctaaaaag agttggaaat ccaatagcaa taaatccaac aaaggaactt ttatctcaaa
     6541 tatcacttga tgaggaaatt tctaaaaatg ttgagataat agtagaaaga aaagatatta
     6601 tttattctct atctacagat gtaaatatag tggatattta atttatacta tctattttaa
     6661 aaacttatat tatatctaat atttatatct aacatataaa agctttgact aaaaatcaaa
     6721 gcttttatat gttgcactaa gaatatatta aatttagctt tatgctttaa tgtatattca
     6781 tatatatcat ttacacttat ctaaactaca atttcattaa aattaaatat aaaaatcaac
     6841 tattattttt taaatagcat ctttcttaat ctattattgt aataacctat aaaagaactg
     6901 taaacatagt catatactat aaaggctatc tcaaatgaac ctataataaa tatgtttatt
     6961 ggaatataaa ctattgttct taataaaaaa tacaatataa gtatcattat atttgcaaat
     7021 actaatttca taaaatactc aatatatata gacctatctt tttctataag atattttaca
     7081 ataccatata ttccaaaggt aagtgaatat acaatccatt gagacttact tgccatcact
     7141 ataaatccta aaactataga acctatataa aaagcaattc cactcttaaa gccccattcc
     7201 attattacaa ttgatactaa aagtgatgct aaccccataa taaacaatgt atttacagga
     7261 ataatgtttg taagagtcaa caatatcaca tttaatgcta ataaaactcc tccataagca
     7321 acttttatac tcatatttat catcctaaca acaagttatt aaatctccac caaaacattc
     7381 acaacatgaa tctaaacacc aaagtttagc acaattttca caacaatcgt cacctagaca
     7441 acaacagcaa tcatcacagc aacagcaaca acatccatct aaatcattac gtcttcttct
     7501 attataattg tcagccctat tgctataatc atttctatat tgtgtataac ttctaagtgc
     7561 atcactatat tcactatttt caggctccat aaattttgct ctttttaaga agtcttcacc
     7621 ttcttcataa tatcctatgt tcattgcaga aaaaccagtt aaataatacc actctgcaca
     7681 tttgtcagtc aaagttttta aaaaatcata tgctttttta aattcactat tttctatgaa
     7741 tgatcttgct ctcataaaat tttcattatt ataagtatta ctgtacattc tttccacctc
     7801 tactttctaa tatattaata tatcttaaat aaacaccaga ataaattata ttttctatta
     7861 ttccaacatt tttatttaga tttagattat caattcccct agctaaaaat cctaatgatg
     7921 ttactattat tttttttact ttttctttta actcatcatt tttatcaata tactctataa
     7981 atggattata tcttcctttc ttaaaatctt catctaaatc ttcataggca tctagtagat
     8041 atatatactt acctatatta aaacctatca ttcttaagtt tctctcattt tcatcttttt
     8101 tatagacaaa tacttcaccc attatatttc caaatatact agatacttca tctatattta
     8161 tatttttatc tttttctaac ttatttaatt catttagttg ttgttttata tactctgctt
     8221 tttcaggata cttttcatat gctaatttta atttgttttt atatatataa tatgccaatt
     8281 tatcttttat gcgtttatca tccattaaat tatcttccaa tttataatac gttaaaagaa
     8341 tattcatact tgctgcatat tctgtaatat catttgtaat tacctttttc tttttaaatg
     8401 gatttactat acacacttcc tctgtactta tagactctgg gttatatact gctgttaata
     8461 aaacaattaa aaatgttata tcatagttca aagaaaacct agagatttct ccatgatttc
     8521 tttttaagta tttacatagt ccacaatagt aagctttgta ataatcatat tctctaaatg
     8581 ttaaatccat tttgtttatt ttaacatatc caaacataaa tactcctttt tgtactattt
     8641 tttatttaga ttatatatag tataacactt tttatcaaga ttatataatt tacagtattc
     8701 taagaaactt aaaaaattcc ttctaatcat tgtatcttaa atttaaaata attagaaggt
     8761 tgggttaaaa ataatattaa attatatact tatattaact catacaagtt agttatgctt
     8821 atatcaattt aagttttaaa taattggtat atatttaaaa ctacctacag tatccttcaa
     8881 ttcttttttt gctttgttta taaccaattc atcaagcatt acatcattta ttgaacatga
     8941 tatagattta gatgccaata acattgcttt aaatcccata tcagaacctg cactggcaca
     9001 agattgccat gtatgggcag aaataccaat aggccaaact gaacaagaac actgtgctgt
     9061 aggaattatg tagctaacat ctccaatatc taatgataat gatatacagt tattatgagt
     9121 aaatgtatca tccgtaactt catcatgcag attcatctta gtaatatcct tatttccact
     9181 taatacacta gaaacactac ttcttttatc tatagacact gtctcagcta atttatctgc
     9241 aaaatagtag tcatcttcat tatagttttg cacacctact aatttcatat tttgtgatag
     9301 aatatctgtt aatattgtat ttggaatata gtcatatact ccacttataa tgtcatattc
     9361 cataatagta ccagtcatta atgctgctcc ttttgctata tcaacaatcc taccaaatac
     9421 atctcttaca tgctccattt taactcctct tatgtaatac cacacttctg caaaatcagg
     9481 aactatattt ggcatttttc caccattagt agttacatag tgtactctac aattatcaaa
     9541 tatatgttct ctcaaataat tacaacctat attcataagt tctactgcat caagtgcact
     9601 tcttccgtta taaggagctt gtgctgcatg ggcactaatc ccattatatc taaatttcac
     9661 agagtaatta gcaagtgttg gtattcttat tggagtattt atatcaaatg gatgccaact
     9721 aaaagcacaa tcaattccat caaaaaaacc tttttttatc atcaatactt ttccagataa
     9781 atcttcttct gctggacatc catagtattt tatagtccct ttcagctttt ttagtttgat
     9841 taattccttt actgcaatta cagctcctat tgaacctgta ccaagtaagt tatgtccaca
     9901 tccatgacct gcaccaccat ctacaatagg ttctttagta gtggatactt tttgagacaa
     9961 accatctaaa gcatcatatt ctcccaaaac tgctataact ggtctacctt ctccataact
    10021 agccacaaat gctgtatcca taccttctaa tccctctata ctaaaaccat gtttttttaa
    10081 acattctttt tgtaaatttg aagcatagta ttctttatac tgaagttctg gatttttcca
    10141 tatcttatct gatatatctc ttaattctcc ctgtatctca tctatatatt ccatcaaaaa
    10201 ctttttattt aaattatcca tattaattat ataaacctcc ctgtttctaa taacttatcg
    10261 attatcagcc aaaatgacta attttaactg atttaaaaat attttctacc atacaaccat
    10321 tttatacaat aaaacctctt gacttgtaag tttcatttta tcaagtaaga ggttttcaag
    10381 ttttataaaa tttttagcat gttaaccaat caagcatatt aacccataat ttattatagt
    10441 atttccaatc agtaaattct tttggacccc agtgaggtgc acagtcagaa ctaaagatag
    10501 cagattttcc ttttccaaac tcgccaacag ctacaaatgg gtctcctcca atagttgcta
    10561 aaacaggaca attatctcta gctactgttt tgttatatcc taaaaacttt ggccattctg
    10621 ttggtatacc ttttaatact ggatgttcag agtcaataac ttctggaatt attcctgctg
    10681 gtttttcaac tctatcatct ttatctatca ttgttattgg taatacatct ttgatagcag
    10741 tttctccaaa tctagctttt gcatctattc ctgtaaatga catatatcca cctaccatga
    10801 ttaacgcccc accattattt acatactctt tgattagttc aagtctatct ggattactat
    10861 tgcaatctat aaaagtacta ttagataata agaacgtatt agaacctata tcagataata
    10921 ttatacagtc atactcattt aactcttcta atttatacgg aaaactatct gctgctacat
    10981 gtgcaggcat atgaactgtc tcatatcctc cagattctat tgcttctttc aaccacttta
    11041 cagcttcttc ataatgagtt gtaacaaacg tatcaaaccc ttttatatga gttatatttt
    11101 ttacccaaga ttcccctact attaatactt ttttcattta tatattcctc ctacttttat
    11161 aagtttaata ttagacttgc atacataagt atactatcta aatactcctt aatttcaaca
    11221 tattcatcaa ttgaatgaca ttgttctatt gaacctggcc ctaatataag tgttggtatt
    11281 tcagctaaat ttttcattac tctcgcatca ttgccagctg ttgaaccttt tatctctaat
    11341 ttttttccta gagtttcttc tagtatttta tgtgcattca ctacaaaatc agaatccttg
    11401 tccatctcaa aacctaaccc tgattgatat atgtttatat ttggtttatt atctttaagc
    11461 cataaatctc cattagccct tgtcattatg acatttgtta catcatttac aacttgttca
    11521 taactcatta tatttggcaa gaagtgtaca cataaattaa atacacactt atctggcaca
    11581 gtagaacctg ctgttccacc atttattaca cctaaattta ttgtaggtga aggtaatagt
    11641 ggatgtttat atatcataag ccagttatgt tccaactctt ttatatcttg aagcaacaac
    11701 atagccttct caattgcatt tacaccctcc cacttactac cacagtgaag agacacacct
    11761 ttaacctcaa cttcaaagaa tacaaatccc atatgtgcaa ctattaagtt ttgttctgat
    11821 ggttcacaaa ttatgcaaca atctccatca ataccattca taactgcatt tatggtacca
    11881 tttccgccac cttcttcatc tactactgac attattttta cattaccagg tacatttaga
    11941 ccactatctt ttataagctt tacagctaat attgatgcta taagtccaga tttcatatca
    12001 gcagttccaa gaccataaag ttttccatta tcttctgtag cacgatatgg attatatttc
    12061 cacttactta tatcacccgg tggcatagtg tctacatgac cattaaatac aattgtctta
    12121 ccaggtaaat catcactata cttacaaatt aaattatatc tatcttgata attgtgacct
    12181 agatttcctt ctttatatat attttttgct tttttaatta attcttcgct catttcttgt
    12241 ctatccacac taaaacctat gtaatttgca agtttttcaa tatattcctg tccttctttt
    12301 tcaaatccac ctagtattcc atgaccaaca tcttcagtct ttatacttac aagttctttt
    12361 aaataatcta tgtattcttg ttttttacta tttaatgtat ctaagagaat ttttttcata
    12421 tttcctccta agatttattt ttacatttaa aatttcaagt ctaatctcat ctatttaagc
    12481 catagtaaac tttttcactt agcatattct cattcaatgt ataaagatgt atgcaaatta
    12541 aagtataaaa atatatacaa aaacttaata atttttatag tttataagct aaaatttaat
    12601 tactatttag atttagctga tttttttaat tttatattat ttatccatat agcaaataac
    12661 attaccaaac cttttataat catttgatta tatgaagata cacttaataa gtttaatcca
    12721 tttcctaata ttcctattat tccgacacct atacatgtac ctactataga accagaacca
    12781 cccatcatat tagtaccacc aagcacaacg gctgctatag catccaattc tgctccatct
    12841 ccaacagttg gtgaacctga tgccaaacgt gatgatagta ttatcccagc taatgctgtt
    12901 agtagtccag ctagtgcgta tacttttact ataactttat ttacttttac tccacataat
    12961 ctagcggcct cttcatttcc acctactgca tgtacatgac gtccaaaatt agttcttttt
    13021 aatagaaaat gtcctgctat tacaactata atcataatta taactggtat tggtattggt
    13081 ccaatatatc ctctaccgag catcttaaat gcatctgtat ttactgatat aggtcttcct
    13141 cctgtaaata caaagcttgc cccacttatt atcgttaaca tggctaaagt tacaatgaat
    13201 ggaggtagtt tttgtgttga aataattgca ccttttatga ctcctaatat tgtccctatt
    13261 gctaatgcaa gtatcatacc tacccatata ggtgcattat aattcactac tgttgtagcc
    13321 caaaatgtac ctactaaagc aattactgct cctacactta aatctattcc tcctgtaatt
    13381 attacaaatg tcatacctac tgcaagtatt gcatttattg atatttgtct tccaacatta
    13441 attaaattat ctaaagttaa aaagttagat gaagctacac tcattattat aaaaattatt
    13501 acaagtccta cagctgcact cccttggtct ctaaatacac ttgttaattt tgtcaataat
    13561 ggagaagatt tttcttcttt atgcttagtt attgaactca ttaattttac ctccgaaagc
    13621 caattttaat acattatttt caaccatttc ttcaccttct aaaaatccac ttatatgacc
    13681 ttctctcata accattattc tgtcactcat tgatattaat tctggtattt cagaagatat
    13741 cattattata cttacaccac tctccactaa atctcccatt agattgtata tctctgcctt
    13801 agcatttaca tctatacctc tggttggttc atccataatc aatatatctg gttgtaataa
    13861 aagccatctt gctattatag ctttttgttg gtttccacca cttaaacttt gtataacttg
    13921 attcatatgt ggtgttttta tctttaaagc ttctatgtat ttttctaaaa tctctttttc
    13981 cttttttcta tctataattg atttaaactt agatatcttt tttaacccag ccatagttgt
    14041 attttctttg acactcattt gtaaaaacaa tccagtacct tttctatctt ctgttattaa
    14101 tcctagtcca ttttctattg catctgatgg agttttaaac tttacttctt cattgtttaa
    14161 atatattttt ccagaatcat atgttctaag tccaaatata gcttctacta tttcacttct
    14221 cttacttcca actagtccac ctattcccaa tatttcacct tttcttatat taaagctaat
    14281 atcattaaaa acattgttct tagttagatt ttccactcgc ataatttctt cttttatttc
    14341 tacttttctt tttgggaaaa attggtctag ctttctatct atcattaaat taactaattc
    14401 ttcctcagac gatacatctt tggtatttaa agtcgaaatc gttttcccat ctcgcattat
    14461 agttattctg tcacaaatct caaagagttc ttccatccta tgggatatat atattatact
    14521 aactccactc tcttttaaat catttataag tctaaaaagt atttcagtct cttttttact
    14581 tattgaagaa gtaggttcat ccattattaa tactttagtt tctgatgata aaactcttgc
    14641 tatttctacc atttgttttt gagctatact aagctctttg actaaactat ttggatttat
    14701 atctattccc atattatcga aaatcttttt agcctcttca ttcatttttt tgtcattggt
    14761 taagactttg ccatttattt cttttcccat aaaaatattt tgtgctactg ttaattcgtc
    14821 aaaaactgtt agttcttgat atatggcatt tatacctatc tttttagaga tatctgggga
    14881 tatattctct atttctttgc cttcaaaaat aaattttcca gaagttttcg aatgtgcacc
    14941 agttaaaatc tttatcattg ttgatttacc agcaccattt tcacccagta gtgcatgaac
    15001 ttcgccatga aataactcaa aattaacatt atctaatgct ctgacaccag gaaattcttt
    15061 agcaatatta cttaacttca gaattatatt actcataatg acactccttt ttaaatagga
    15121 tttctttcaa ataatctcat tttattttac taattcttaa agtcttttat attttcctta
    15181 tcataaagta aagctggtac tggtatattc ttttctacct tttccccttt tatagctttt
    15241 actactgctt ctacagctaa acgtcccatc tctttaggtt gttgttgaat catagcaaga
    15301 atagttccat ctgttattcc acttgatgct tcttcagtta agtcaaatcc tacaatctta
    15361 acatccttat tttttgcaga ttcaagtgca gctttagctc ctagtacact attttcagct
    15421 gttgcatata ttatatctac atctggatta gattgaatta agttttctac tgtattaaga
    15481 gattcttctc tgtcatatcc tggttgtgaa tttaatatct taacatttgc tgaacctttt
    15541 aatgcatctt taaatccatc tattctctgc atttgtattt gagattttaa atctgtcact
    15601 attgctattt ctgactttcc acctaacttt tcatctatgt attttttagt gtattcccct
    15661 aattttttac cagcatcata actaactgtt cctacataac ttgtaatatt ttcagtattt
    15721 aatacagcat ctactgtaac tactggcatt ttttcaaatg ctccttctac tgctggtact
    15781 atagccgaat tatcagttcc aagtactatc attccatcta cattttgttg tataaaatct
    15841 tctattgctg atgtttgctt agctacatct tggtctgggt catttactat aactttcaca
    15901 cctaattctt tagctttttc ttttattccc tcatcaatat ctataaaaaa ttgatgttga
    15961 agagttattg tagatactcc aatagttaaa tctttcttgt ctgaaccacc tttattatct
    16021 gatttacttg gtccttccat tgaacaccct gttaacattc ctattgctag tattccagca
    16081 attcctaaag ataaaatttt tctcactctc ataattaacc tccattttta atagttttaa
    16141 atttgaacaa attagatttt tatttattat ttaaatactt ttcaacatct tctttatagg
    16201 gcataccaga ttgtgcacct agtttagtta cagaaagacc tgatactact actgcatatt
    16261 ctactgctgt atataaattt ttcccttctg ttatagccac tgccaatgct ccattaaatg
    16321 tatctcctgc tcctgtggta tcttgaacat caacacttat acaagataca tgcttgattt
    16381 cctcaccatc ataaaatcta gctccatttt ttccttctgt tattacaagt ttatttggat
    16441 atttttttaa tacttcttct atcccttcat ttgtgtcaaa aactatctta tattcatgtt
    16501 catttggtgt taagtaagtt actttttcta ttatatcttc atttagtttt actgctggtg
    16561 ctggatttaa taaaacccta atcctatttt caaaacagaa attcactaca taatttattg
    16621 tttttaatgg aatctctaat tgtagcaata ctatatctgc attctttatc tcttcttcat
    16681 tctttttaat taattctata tctactagct cattagtccc tggtacaaca acaatactat
    16741 tatcattttc acataatgtt ataacagcaa caccagaact tttgtgatta tttctggata
    16801 ctaagctata atcaacttta ttcgttctaa aatttcttat taactcctct cctaagccat
    16861 cttcccctat gcatgatatc atttttacat ttgctcctag tttagaagct gagatagctt
    16921 gattagcccc ttttcctcca ggtgatgtaa aaaaactatt acctaatact gtttctcctt
    16981 tttctggtct tttatctaca gaacatacca tgtccatgtt tacacttcct atgacaacta
    17041 tgtttcccat tacattactc ctttacatat ctatttatgt ctctcgtgct gttattcact
    17101 ttcttttgtt gaatctctta tgaccaattc tggtttgaaa atcaattcat caaacgattc
    17161 ttttttatct cctcttaaac aatttatcaa tatttcactg gctttatatc ctatatcata
    17221 agacggctgt tttactgtgg taagagaagg gcttattaga cttgatgttt gaatatcatc
    17281 aaatcccacg acacttatat catttggtat acttaatcct ctttctttaa gacctcttat
    17341 gacacctatt gcaatcaaat catttgcaca aaatattgca tcaaaatcta tcttttctaa
    17401 attttttata aattcttgtc cccattcaat cttatatttt ccttcaaata cataagcagg
    17461 attgtagctt aaattgcact cgattagagc ctttttatat ccttctaatc tatctactgc
    17521 tatttcattt tgaagtggac cacttaggta tattattttc ttatttccaa tatctaataa
    17581 atgttttgta gctatatagg ccccttcaaa gttatttatt ttaacaattc cttttaaggt
    17641 ttctttgttc atacttatct tcttatcaat caagacagtt ggtattttta tatctttata
    17701 atttgcttct ttccagtttg tatttgaaga tgctgcaaat ataattccat ctatcatcct
    17761 ctctgatagt gttcttattc cttcatattc ttttttagca tcttcatctg tattacataa
    17821 aattatattg tagccttctt cattagcttt atcttctaca cctcttgata catctgtaaa
    17881 gaatggattt cgtatatctg gtataagaag ccctatagta ttagtttttt tagttactaa
    17941 actttgtgct aatttatttg gtacataatt tttttccttc attattttca aaattttttc
    18001 tcttgttgcc tgacttatat tttcatcttt attattaatg acttttgaca cagttgtttt
    18061 agaaacacca gccaattcgc caatttcttt tatagttata ctcaagaagt tcacccccaa
    18121 aatttaggaa accgttttac ttaaccggtt acctagatac tatcacatct ttttttcatt
    18181 tgcaaccatt ttatataaat tttgatattt ttcccttttt tcaaaatatt gcaacttata
    18241 attcaatttt aatatttaaa ttaatctgca aaaaaaaatt aatatacttt tataattatc
    18301 taaaagtata ttaattaatt taagcagtat ttattaaaat tcaaatcctt gctctataat
    18361 cattttttta tcattttcaa tcccacttcc aacagttgtc aaataatcac caacaattgc
    18421 agaatttact cccccattaa atccaacttt gtcatatccc tttaatgata gccttccacc
    18481 agcatacctt atgtacacat taggaattat aaacctaaat actgctgttg tttttagtat
    18541 ttccattggt tctaatacat cataattttc cattggtgta ttcttaatag gatttaaaat
    18601 gtttattgga aatgatttta catttaattc tcgtatttca aaagccattt ttagtctctg
    18661 ttcttcactc tcattcattc caattatacc gcctacacat acatctagcc cagctttttt
    18721 agcatctttt atagttttta tcctatcttt atatgtgtgt gttgtacata tattatgaaa
    18781 attatctcta cacgtctcaa cattgtggtg atatgtagtc acacctgctg acttcaacat
    18841 tttagcttgc tcataatcta taatacctaa agatgcacat aattttaaat tagtattttc
    18901 cctcaatcct tcatagatat ttaaaatatt attaaactcc tttcctgaca tacctttacc
    18961 acttgtaacc aatgagaatc tatgcactcc tttactctcc atctctttag ctctatttaa
    19021 gatttcattg taatctaata atgaatattc ttctacacca gttttataat aagaagactg
    19081 agcacaaaat ttacagtctt cactacattt gccagattta gcattcatta tcgtacataa
    19141 atctgctttt cttcccataa aatattctct tatttcattt gcagatttta atagaatatc
    19201 aaaatcgttt ttattatttg tatcaaggct tattaaatta agagcttctt cataacttat
    19261 ttctttttct ctcaatactt tattttttaa ttttattatg tatttttcca tatatcctcc
    19321 tatttgtcaa cttaatattt ttatgtagtt tacttattat atatagtatt gcatatataa
    19381 catttattgg tcaactttat tttagacaca agtttactat acacaaaaaa atactgcttc
    19441 aaaacaatat tagtatatag attatacata ttattattaa agcagtattt tttattagaa
    19501 tataattctt actggtttat aaaactatcc ttgtcttaac tccatccaag ctttccattg
    19561 taataacttc taagccttca tattctctaa atagccattt taaatccttc atggcttgat
    19621 taacctgact ttttgttaga ttatttataa aatattcttt tctagtttcc tcatactctt
    19681 tgctagtcaa aaagtttctt agaaaattaa acgcaaatac acttggaata ttgtttacat
    19741 tagccttctt gctgttttcc ttctcatagt aaattttcat aaatgcatac tcctttgata
    19801 cttttagatt tctcttacaa taattatatg catttaagtg ctactattct tataatttta
    19861 ttaattcata ttctttactt cccataccca tttcacatgc atagtctatt attctagttc
    19921 catttacatg aggatgttca tgtttgaaaa catcatgacc catttcctta caaatcaaat
    19981 catagcatgc ttgttcaata gctacagggt ctgttgatgc taatattcct atatcatggg
    20041 caattggtct acctgaccaa ggtacacaat cacaaagtgg agttacattc ataacaaaag
    20101 ttatataccc taccttgtct tttttatttg atactgctcc ataagcgtat tctgccatct
    20161 tttcgacaaa cacatctgag tcactttccc attgtatagt tactgctcta gttggacaga
    20221 cagttggaca ttctccacat ccataacata catcagaatc tataactgct tttttatcca
    20281 ctatacttat agcttttgta ggacaagaat taacacactt tccacaacct acacacttct
    20341 tttcctttac tactggagta acatcagaat gttgcatttg ttttcctgct gcacttgcac
    20401 atcccatagc taagtttttt aatgctcctc caaaacctgc tgcttcatga cctttaaaat
    20461 gactcattac tatcattgca gaagaattat aaatttctcc acctattttt acactttcaa
    20521 aatgtttttt gtctattttt acattctcat aatttctact atataatcca tctgcaatta
    20581 ttactggtgc atttacaact gcataagcaa atccattttc tatagcagtt gtaagatggt
    20641 ctacagaatt tgttctactc cctgtataca gtgtatttgt atctgttaaa aatggctttc
    20701 cttcgcagtc tttaacctta tcaaccactt gtctaactgc cacaggactc atatatgtag
    20761 tatttccctt ttctccaaag tgaagtttaa ttgcgacttg gtcatttttt tctattaaat
    20821 ctttgaatcc tgcttcatca aataatcttc ttacattgtt tggtacgttt ttattttgcg
    20881 aatttgagta taaatcacaa aaatatactt ttgacttttc catatctaca cccctttaaa
    20941 atatttactt atactaattt tatcactttt aaagtaattt ttagtatatt ttaacataat
    21001 taaatattct tatatttatt tgatacaaat aatattattt gtattatttt ctacacttaa
    21061 ttctatatat taaactacac ctaaaaatca aaaagacttg aaacttaagt ccaagtcttt
    21121 ttgattttta ggtgtagttt ttaacaacat aagattactt taccttgtga taaaggtatt
    21181 attaaactgg ttttttatta aataaaataa cagctgctag taaacatact atagaaataa
    21241 gaactgttat aataattgga ataataaatt ctggcattgc cactttccct gaaatcaagt
    21301 ctatattttt tgacatcaag aaattaggag tataaggtgc aatctgttta ggaatagaaa
    21361 ttaaactcaa taagagagtt gctaccaata caagtaaaac actggcaaat gctggtctaa
    21421 atagtacaca cccaagaatt aatacactaa tatacataat tcctataagc cataaattaa
    21481 atgctgaaaa tataatatga ttaagatttg cagttggcca aaaatacatt gtatagccat
    21541 aagcaaataa aaaactcaac caaaaaccaa ttgtcataat aatcaccgta acagagaatt
    21601 tagacaatac tatagatgaa cgtgataaac ctttagtcag cataataaca agtgtccctt
    21661 tagaatattc atttgataaa atattacaaa aaataattag agttaaactc ataccaagac
    21721 cagagatatt gctaaaaaat tgtgtccaag aatctaaagc agttggtgca gcagtaactt
    21781 ttaaagtcgg tgcaaaatga gcaatcaagt ctggcataaa ttttgcagaa ataggactac
    21841 ttataccaaa tattagaaat aaaataatta aactaaataa tttataattt cttaaattct
    21901 ctttaaattc tttttttgta aatgctatgt attctctcac tttactacct ccaaaaataa
    21961 attttcaagt gataattcta ttagttccat ctttactgga caaagattca tttctgcgag
    22021 tgtagatata actgtttttt gtattgcttt tatatcttta ccatgcaaaa caatttccat
    22081 attagtttct tctgaatctt ttagtaagga tttaatacca tcactagact taaacttttg
    22141 aatttcatca ttacttgcaa actccagtaa aagtctatcc tttccgtgca tactttttat
    22201 ttctgaaagt gtaccactaa gggcaatgct tcctttgttt aatatagcaa catggtcaca
    22261 gattctttca acatctgata gtatatgtgt tgaaaatatt acagttgttg agtctttgat
    22321 tttaagcata atatctaata tttcttttcg accaacaggg tcaagtgcac ttgttggttc
    22381 atcacaaata agtaatttgg ggcgtgaaag caaagcttga gctataccaa gtcgttgctt
    22441 cattccacga gaaaacccac ctattcgtct cttttcattt ccaagaccca caagtgataa
    22501 caactcttca ctccttatct gtatttcttt ttttgaaagt cccgtaattt caccacaaag
    22561 agaaagatat tcaagtggtc tcatatagtt gtaaaactct ggaacatcag gcagataacc
    22621 tacatgacga ttagaacttg ttttcccata ggtcactttc tcaccacaaa catctatagt
    22681 tccaccatct ggtttaagta acccaagtac cattttcatt gttgtagtct tacctgcacc
    22741 attcttacct acaaacccaa agactgaccc ttctggtaca gtaaaattta aattatctat
    22801 tatctttcga ttaccaaaac cttttgataa tccatttatt gagacaatat tcatcattct
    22861 tcacctcttc ctattgtaaa atacaaaatt ggtcctatta gactaataaa cagtactaca
    22921 ataatccata ttgccttatt tccaatttta taatttggat gtctcaatac atgaacaagt
    22981 gcagtaatta taagtattaa atctaaaata ataacaggaa ccaaaagtgg taagtattcc
    23041 atcaaatttt ccatactctt attcctccaa taaagataat ttttctataa gaaatttgta
    23101 ttctttgttt tcacttaata cagaaaacat tggattattt ttaatagcag caacaatacc
    23161 tgcttttacg attgtatcat cacgtgtaat acctgttcca atttcagaaa tccatctgtc
    23221 aagtttatta aaaaagtcat ccccatgtat tataattggg tatatatcac gtgttgctaa
    23281 atctacatat tttcgtaatg atttaatagc tccttcatta tcaccatgta ttaaattaat
    23341 ttgtgcctgt gttaaatatg aagaaagcat aatagcagga gataatgtat caagctcaaa
    23401 tatatctgaa attgctagaa tacgtctttc tatttccttc atttgtaatt tatctgcatt
    23461 ttgcaaaata cccactagat tttggactaa tgatataagg ttctgatatg caccaagttg
    23521 aaatgtttct cttgcttcat ccatttgacc attcatcatt tgcccttgtg caagaagtat
    23581 agattcattt atcatgggaa aattgctgtt ttgcaataaa tcaatcactt gtatgggttg
    23641 atttaacaat atataacaag ttgcctccat actttttgct tgacggcaaa tatctatgtc
    23701 attacaagtt tcctgtatac gaataaatat ttctaatgct tcactaatta atagttcacg
    23761 ctttttttca tctacaatat catagtgatt aactattaaa agacccatcc gaaaaatcaa
    23821 aggaaaacat gaataatatt tttttatgat ttctcttatt tcatccatga cttcatcaaa
    23881 tggtttaacc gtaaagtctt tgcacagttt attatacagt ttattaatat cttccttcat
    23941 catttgaggt tcataacata ttaactcatc aacggtaatg ttgaaatatg tcgcgagttg
    24001 cggtaaaaag gttatgtcag gatagctttg tccagtttcc catttagata cagaagcttt
    24061 agaaacgcct atataatttg caagttcatc ttgtgtaatt cctttttctt tacgctttaa
    24121 aatcagcgtt tttgcgatgt ttaattcatt catgtcaatc cctccttatg tataattata
    24181 tccttaaaca tgtaaagcta acaagcgatt tgaagttgac ttttgacgaa aaaatcaacc
    24241 acagagaaac aaataaacaa gtatataaaa tatatattta aatttatctt taatctcctt
    24301 tgtaaaatat gtaaactttg taaataaaat ccatattata taaaactaat agttaatcta
    24361 tctaaataca gtttttattt atcacgtttt taaatatttt gtgtaaaacc tcttttaata
    24421 taaacacctt attttcattt ctcatttcat attcttttta aaatactgaa tataactctt
    24481 cgattttcca tttaatcctt ttataaagtt attattcgta tttattcctc taattataac
    24541 aggcaactat catgtattgg tatttgtcta ttatataaaa atctataaaa gaaaagcagt
    24601 caatcattta gattaactgc ttgtacatat atcttattaa actgtataaa ctataatttt
    24661 tattattctc cattttcatc ccaagtcata aatccccatc cactatctgg ataaatatcg
    24721 atagactttt ctctaatcat ataaccaact ttataacact cttttgttat tttattagat
    24781 agtatacatc cttctccatt tatctaattc catttaatta acttttcttt tcaaccttaa
    24841 cttttataaa atcatctttt ttaataattt catacttatt ttctcttcaa catcatcaaa
    24901 tgagggtgtc tcaaaattaa ctttttactt cataaggcac tcttttttgt atgaagtaaa
    24961 atatattttc atcatttcaa caataagtaa gaggcttatc tctattttga gacagcccca
    25021 tttgatggtg ttgaacattt aaataaggtt gaactcgttt tatctctctt tttaagatat
    25081 ctgtaaattt gcacataaag tctcttcttc ttttacaatt ctaatagttt tattaaagcc
    25141 aaagtatata tctccatatt ctttcgtata tgatttattg gtactctttc atctactcca
    25201 tgaccaccag ttttatacgc taagaactta ggtccaaacg ccaccatatc tttaaaccaa
    25261 cttgcatatg tgccaccacc catagcaatt gtatatccat tttcatctgt tacttcttca
    25321 taggcttcta ataatttttt aagtactaaa ttactctcat ccacataatg taagtcttta
    25381 tgagcaataa cctctatttt ccaaccatta caaaaaacct ttttgaatct atctattata
    25441 ttgtttaaaa ctatattttt accatacctt atatctaact cacatacgat tttttcctca
    25501 tttactttta atattcctaa atttaaggta gtgtttttga atacctcatc atattgatta
    25561 atccccaatg catctccata ataatcacta caaaggtatt ttgaaactaa ttctatatct
    25621 tctctatgct gaaaatccat attatcctca actatcttta ttaccatttt agatatagca
    25681 ttttctccct tttcaggatt acttgcatgt acagctactc ctttagtagt taactctgaa
    25741 ataattccat taataatttt tgcagtacaa tgagatggaa caacattttc tttagttcct
    25801 ccacttacta ttatgtaagg tttacttttg tcaattttaa tttctttttc tatccttata
    25861 tgaacccttc ctttttctcc attaaccact ggatacatag catctggagt aaatcccata
    25921 cttggaattt cttcaccttt atctaagtaa tatttgatac atttcatacc tgtctcctca
    25981 tttgtaccaa aaattaatct tattctttta ttaattgtag gatacatatc ctctatgttt
    26041 ttcaatacat gtataagcat agatacagca cccttattat ctatgacacc tcttccataa
    26101 ataatattgt caattactgc tcccgaaaat gggtctacac tccacccctc tcctggtgga
    26161 actatatcta aatgcattgg aatccctatt aattcctctc cctctccaat ttctgcatat
    26221 ccaatataat tatcaatatt tttaacacta aaacctaaac tagcacagta atctaaagtt
    26281 ttatctaaag attttttagg tcctatacca aaaggacatt gtgaaatagg ttcaccttgt
    26341 tgtgaatcaa tttttatcaa ttcttttaga aattctatgg ttctatttaa actattatct
    26401 acacttctgc ttatatccat aaatcaacct actttctatc tttttaaagc ctgtccttga
    26461 agaacaggct tttagcatta aaaattattt ttttgtaaat actaattcac aaacttcatc
    26521 atcatgagca catttttgag caaaatctaa tttaacacct tgtgcacagt caactcttcc
    26581 ataatcccca taacaagcta atctacaaat ttcttttctt tcttctttag ttaatccata
    26641 ttctttccaa gcagcatcta atggacagtg atggaatttt aaaacgccat tctcatctgt
    26701 attttctact aattccattt caaacacttt ttgtccttta ctgttatata acatttctgc
    26761 catttttcct ggagtatctg ctacgttata tttttgacct ctcattttac caaatccata
    26821 tattgcttct ttacatatat catctggatt agctcctaat ttttcagctt ctttcataag
    26881 aagatagaac catactgctc tttctcctgt tgctcctcta attccttcta aatattcctt
    26941 ttctgtatac attttttctg acatttcttt ctcctcctaa aattaaattc tatattttaa
    27001 tcactgtgaa ttagaatatt ccaatgaatg accctaccaa tgctaataca actaaaatta
    27061 ctaataactt agttattgtc cacttcttgt atttcattcc ccaatatacg aagaatacaa
    27121 caccaagtgg taataaacct ggagcaatca tatttaatgt atcttgaaca acaattgctt
    27181 taccaccaat atcaaatgat agcggagttg aaagcttaac tgtagttgca gataatgctc
    27241 ccatcataaa cattcctaga attgaagcac catcaattat cttattgaca ataccacctg
    27301 atagaatttt cttaattgaa tctctaccta aagaatatcc taaatgaaat aagtaataac
    27361 cttcgcaaaa tagtaatata ctaaacatta ttgggaaaaa tgcacctata acacttcctt
    27421 ctgctccaaa tgaagcacct aaagctaaga atattgtttg aaatgtacca aaatctaaag
    27481 tatctccaat tccagccaaa ggtcccataa gaccattctt aacacctgaa ataacttctc
    27541 ctgggatttt tccttcagta gctttctctt cttccatagc taaaactgta ccgtgaatta
    27601 atcctcccca tatagcttga gtattaaaaa attgtaagtg tctctttaac gcatctacta
    27661 aatcttcttt tctcttatat aacttttcta aagccggagt gaaacttgca caaactgcta
    27721 atgcttgcat tctttcaaaa gagttggata attctgctgt ccaccaccat cttagatatg
    27781 tttttgttac atctttcttt gtaagtactc ttgtcttatt aacttcagtt ggaccttgtc
    27841 catttttaac ttctttattt tttatttctt gcattatact tcacctaccc ttttgtcttc
    27901 tttcataaag gtaattaaaa gagctataca aacaccaaat attgcagctg cagtaattga
    27961 tatttcaaaa tattgaacca taaaaaatcc tataataaac attggtaaaa atttattttt
    28021 acctatagta aatattgttg ttgcaaaacc taatgcaggt aatataccgc ccattactgt
    28081 taatccattc attaaccatt gtggtataac atctaataat gattgaacta aatctgctcc
    28141 aaaaaagtta catacaaaaa ctattggaaa ccttaaaaca aacccaaata ttaaagaata
    28201 aagtgttgca catctccaga tgccttttgt attaccttct tcagcatact tatctgcctt
    28261 atgaaccaat acagcattac cagttcttct taagttatta actaatactc ctataacacc
    28321 taaaggtacc gcaatagata ctgcaacttc tgcattaaca ccagtttgaa gtgctactgg
    28381 tattgcaata agagcagcta gacatttatc tgatggaata tttccccctg ctgcgaccat
    28441 tcctagataa accatttcta tacttgcacc aacaatcata ccagttgtta catcacccca
    28501 aagtaatcca aggctaaaac ctataaataa aggagacttt agtgttgaga aaagggtata
    28561 ccctgcaaga cctgctgcaa accaatacca taatcctgtt acaattgcca acatcaaaac
    28621 agattccatt tataattcct cctaatttat attttttatt taaatttctt taacgcattt
    28681 tcatatgtca tagatgattc ctctggtata acttgtagga ctatttcaac cccattttca
    28741 ttaagttcat taagttgttc tacatcctca tttcctaatg aaacagctgt aaacaccatt
    28801 ttctttcctg cttcatttgg aatacctcct aattgaatct tttcatattt aagtccagct
    28861 ttataagcct tatttgccat atcaacattt ttaaatagaa taaatatatt tttatctcct
    28921 aaagtgttgt tttggaatgc ttgtcccgca tcttctgctg ttaaaataac tacttctact
    28981 cctgatggtg ctgccatttt atatatgtca gccataaatt catcttgacc taagaagtca
    29041 tcaactatta ttattttttg agcttgagcg atttttaccc attttgttat aacttgccca
    29101 tgaattaatc tgtggtctac tcttactaat gttatttttg ccattatatt cacctcataa
    29161 tttatatttt tttaaattct ttaattaaat ccacacaaag gttattattt tttttgacga
    29221 ttctttctgg taatgtatgt aatttatctt ctttgcttaa ttcttcatct acgattaaaa
    29281 ttgtctgtaa gctaagtcca tttattgcat ctattcctct tcttatagca acagctattg
    29341 caacacttga tgtagatcct ccatataaat ctgatattat aattttcaca tcttctttat
    29401 ctactacttc ttcaacctct ttcataaata cagaaacatc tttttcagaa cttagtgata
    29461 aaacttcata ttgatttgac aatccaaagt ttttatttgt ttcttctact aagacttttc
    29521 cccaagttcc atggcacagt atgattacct tatatttatt cattgctaaa taaactctcc
    29581 tttatagcga ttaatatttt ggtttaattc tacttcttct tcatcaggta ccatttgaat
    29641 tgatacatta atattctttt taataaattc atctattaaa agaaactctt ttttattaat
    29701 gtatacattt tgagatattt tcttttttcc tattcctgta ggtattcttc caacaataag
    29761 ttttttgata ttaatccctt cttctatcat tctatttgca gtatctaaat ttttaattaa
    29821 tacaaaaacg ttatcatcag cttgattatt ataaaaatat ctccttgatt cacttacagt
    29881 taatattgat aaatcaatat tctttggtgc tgccattttt atcaaacctt tagcaataac
    29941 atcattggtt gtttcatcat ctactactac tatctttttt acacctagat gcttaaccca
    30001 tttatatata acttgaccat gtattaatct atcatcacat ctgcaatatt ttactaaaga
    30061 catttttaat caaactcctt tttctattac attgagctaa gaactttatt aatattgttt
    30121 ataccagttt gtcctgcttc aatacatgct tctgctaact cttctaaact ttttccctct
    30181 ctattaatta aagcttcaag tagcattgga agatttactc cagtaagtgc atgaaacttg
    30241 ccaccttttt taaggtttaa acttgcaaca ttacttggcg aacctcctaa taggtcagct
    30301 aatactaaaa ctccgtcacc ttcatctaat tcatttaact tttctacaaa atttttattt
    30361 aagtcttcaa tattatcatt cctatttagt ttaattgctg ctgttctttc tgctggtcct
    30421 gcaataagtt cacagctttt tataatttct tcactaaaac tcccatgagt cataactatt
    30481 attcctatca ccaatttcac ctcacttttc ttgtcacttt ttatataagc aattgccgtg
    30541 ccaacttatg tgtatcaata acaaatggct attttacagg atttaatcaa aactttttac
    30601 tcaatttttt ttaaaaactt attatttcga tatgtatcaa aagatttttt caacctgtat
    30661 caaatctgta tcaaaaaaag taaaaaaaag atagcctttt ggctattttt aaattttgta
    30721 tcaacttttg aatttatctg tatcatattg tgattcaaat atatctaata tatttgcata
    30781 ttccatatct gaaataatta tatcgaatgt ctcttctact agcttaaaac tttcttttat
    30841 aactaaatat aattctttat ttctactaat tctttcttca tatgatttat aaactaaaga
    30901 ctcttttatt attactcttt caatcataca tgaacaatga aatataaact ttactaaaag
    30961 ttcatcttga attactaaag aatagtgttc actaattcta ttaattactt cttttaatat
    31021 aaaataagcc ttttccccat ttaaaaaagt caaagtttta ctaatattat caattagtaa
    31081 tctatcaaaa tatctaccac cattatcttt ttcagcatat gtaacttcca tatctccata
    31141 atgtttcttt atagattcta taatattaaa atataatttc tctaatgtca tttctggagt
    31201 tagagccttt ctagttgctt ctattacaat aggtgtactt accatatcta ttgcttttac
    31261 ttcttttcct gtagcatcac gaataacttt agcaaatgtt aaaatagacc ccatatcagt
    31321 taaaattaaa attccttttc ctttatcaat tttattggct atttctacag cttgtactaa
    31381 aatatcctga actctatctt ctaatcccat attaattgcc actgcatggt tacaatccag
    31441 taaagtattg gctacattca ccatgctaga tgctgtagat tcaccatgca ttatgactaa
    31501 tattgctata ctctcctcat ttaatcccac atacgataaa taaagaaatg ttgctaaaaa
    31561 aattgcttca tcttctggaa actctactcc atacattaat gacatgttag ataatatttc
    31621 ttttgctatg ttatattcat atgctctttc cttcataatt tcatctttat taggatgctt
    31681 tactatattt ccaatcttta ttctttcaat taaattctga atatgtaaag ctatagcttt
    31741 tattaattta tctaatcctt ctcttttgct ccatttatca tgcttttgta aaatatattt
    31801 tacagtagtt gcaattgaat cattaacaat gttgttataa gctgtttgat tactactatt
    31861 atatataaaa gtattcatta aattatagga atacttctgt atgtctccat ctataacact
    31921 tctaatttgt gactctttaa taccttcttg ttgaagttta ttccaagttt cttttatcat
    31981 tccataaaaa tctaaatcat attttttatt ttctattaat aaattttcac tttcaaaatc
    32041 cttttcttct ggtaaaaaaa taatatcatc ttttccaata aaactgaaat tttgtacaaa
    32101 ctcctctttt ctattatttc catataatga ttctctaata ttatttggta gaagagagag
    32161 ccttacatat acacattccc ttttataggt catataatct aaaaaagctc ttgcacaaat
    32221 taattgtata tcgcttttta attggcctat gtttccctta cacttatata aaataaatgc
    32281 cttaataact tcttttgaaa cctttaactt aacaccaatc ctaacactct cttctttaaa
    32341 aaaataacat ataagcttca ttctctcttg aaagcctctt tcctctaaag acggaatctt
    32401 tatagttaca ggtattcttc ttaaaaacgt ttccaacatt gctgtttctg ggtcttctgt
    32461 agtggctgca attatcataa ctctacactc gtgtacctta ttagcctctc ctaaccttct
    32521 atatatccct ttatccataa gcagaaatag catttcttgt ccctctgcag ataatctatg
    32581 aacttcatct aaaaacaata ctccattatt agcttcatgt acaataccat acttatcttt
    32641 atcagctcca gtaaatgctc cttttacata tccaaaaagc tgcgctaata ataattgctg
    32701 attatcagaa taatcagcac agttaaatat gataaatggt gcattatcac tatcttcttt
    32761 tatagttaaa ctatacttat acatatattc tgcaaatata gatttaccaa ctcctgtagg
    32821 tcctattatt aatgtactca accccttagg tggatatatt actgctgctt tagcctgttc
    32881 tatttgggac cttaaacttc catcacatcc tactaaatta aaaatattgt tctctttttt
    32941 tatactctct aaaatatact catctttgat agaatcattt tcactatatt cgtttataga
    33001 gttaatatta tcaaatatcc ctttatttag tttctttcct aaaatactct caatactttc
    33061 tttatataag tactgaacag gcttaccctt tatctttata ataatacttt cttttattaa
    33121 ttgatttaat tccttactag cattacttct atttatacct acttcagtag ctatatattc
    33181 tgcatcaaat ccaaatctaa tgccattatt aaagttcaag tgttcttctg ttaacctttt
    33241 caagcaaatt aaaacttttt ctttccttga tagcttcttt tgtgtcatag ttaacctccc
    33301 ctataagcag taatactgtt aaacatttat tattaagctt aattctacaa tattttaata
    33361 tttttgtcta attatgtaac tattgatttt ctttcctatt cgttttataa tataaattac
    33421 gttaccctca tcttttatag aggaggaatt tatatgaatt ttttatcaga tttacacacc
    33481 cactctatag tcagtggtca tggctatagc actttactag aaaatattaa ttattgtaaa
    33541 gaaaatggaa ttaagatact aggtacatct gaacatggtc ctaagatgcc tggttctccc
    33601 cacaaatggt actttcacaa cattcaaaat atcccaagaa tcataaataa cattataatt
    33661 ttaagaggct gtgaagcaaa tatactagat attaaaggaa acatagacct agagcctttt
    33721 gtaattcctc ggttagatta tcttatcttg tcatttcacg aagctgtgtt ttctccaaat
    33781 acattagaga ataatactaa agctttaata aatgcaataa ataagcatga taatattgaa
    33841 atattaggac accttggaaa ctcaaactac ccaatcgact atgaattaat cattaaatta
    33901 gctatagaaa agaatatctt aatagaaata aataattgtt ctattaaagg cgtgtcaaga
    33961 aatggtagct cggataactg taagtatatt gctacacttt gcaaaaaata tggtgctaaa
    34021 atcatactga cttcagatgc tcacatttgt tttgacatag gtaactatga gtattctgaa
    34081 aatatcttaa aggaaattaa ttttccagat gaattaataa tgaattatcc taaaaaactt
    34141 attaatcatt ttcaccaaaa aggtaaatta ctagatgttg attatacaaa tatctaagta
    34201 ataatacatg cttactttaa ctcccataaa aattttaata ctatttatat tatatgaaat
    34261 aaagaaatta taataaagaa gctatttaat aaagcttaat tacttttatg aacttgatgg
    34321 attgatatgg tctatgatag caatagaaat tataacattt ttaataaact tagttttata
    34381 taccttatag tataaatcta aaaaagcatc atctaaaaag gagctgtcac atgagcaaat
    34441 taaatttgtt ctatgatagc tcttttttta cttattttaa ttaggtatat ttttaattat
    34501 tttatataaa gcttatgttc taagtaaaat tatgcataac aaacaaaggt ataaaaatac
    34561 cattttttca gataatattt aattataaat aataaattta gaatacttct tttaaatgat
    34621 gagagtctag aaatttttat gtgtattctt taaataaaaa tttccagact ctagcccctt
    34681 tttgaatatt ttaaaattta acttaaacta agcaaattct ataatagctt atctctaaat
    34741 atttaaattt aatttatcat acaatattac ttattataaa acttaaaaca tataaaaata
    34801 tattttaaag caataatttt attttactta ttaaatattt ggagttatta aaatcttttc
    34861 tatctaaaaa atattgatat taaatatcta ataagaaaat ttaataagaa aaatatagat
    34921 tatatcaaaa aaatattcta ttataatctt tagaacaaac agtagtaaat gttatcttca
    34981 taaacaaatt tatttttcac aagtacaata cttatttaaa aaaggaggtt taatatgtta
    35041 agtaaaaaaa atgtaagttt aattatttta gtaataattt taagtttatt aactgctata
    35101 tttattttca ataataaaaa tactaacaat aaattgaaag aagttactaa gaataatgaa
    35161 gaaatagtac ctaaaaactt agaaggttta agtatagaac aattaataga gattagagat
    35221 aaagccattg ataaaaattt agaaggtctt agggaatttg gagaagattc agtaaaaaaa
    35281 cgtaaagatt caattttaaa ctttgaattt ggattagtag cttttgttaa caagaattat
    35341 agcaacttaa cagaaaaaga gaaattaata agacaaatta atattgttaa ctatgaaaac
    35401 tctataaatg atataatgga tccttcaggt tcattctttg aattagcaaa tcagaattac
    35461 caagtgaatg agtttctatt aaaaaacgga aaagattata aagaatggtc atatgaaggt
    35521 ctagattttc taattgatca tctagaagaa ctgtgcaaat ttgttgattt tgatgtaaaa
    35581 gatgtaatag atattattga tcctacaata aaaacagacc tttcagagga acaaaagaaa
    35641 tctttgaatg ataaattaaa gaaaatatct tcaagtgata ctttaagtga agaagttaaa
    35701 aaggagataa aaaactggga aaacaattta aataatttaa atatgaataa aaataattct
    35761 aattaatttt ctcataagaa aaatgcttat atcgtgataa tgtgaaatgt acataattat
    35821 aaacaaaaaa ggggctgtga cactaaaaaa tagtgctacg ccctttttta tttgtaataa
    35881 tcatcaatac aaattatttt attatatgta attatttatg tcttaaccaa ttttatcgaa
    35941 aataacagtc ttctctttta aagagatttt tcctacttga tatccatatt catttaattc
    36001 ctttttatat ttcaaaaacg aatggtctat ctctatccca gcaatatctt ttatttcttc
    36061 aaaagttaat ttaaacgatt gactattgtt tttcttaaca tattcccata aagaactata
    36121 tttactcatc ttatatcact ccttttaaaa tcatatctta tctattaatt tatattctat
    36181 cattattaat gtataaagtc atgctactta ttatttatat ataaaaatat ccaaataata
    36241 agtagcaatt ttttatagtc ataatatttt tttcagatta aataaaaatc aattatagtt
    36301 aaaatatttt ttaaaagaaa tctatcccat tataaagtaa attttaatta tcttatttct
    36361 agtatgtaat tattcaaaat agtatatcat tattgaaata cacatctaat aactaaaaat
    36421 ttttacatat tcattattta aaaatctttt tattgcattt aaattaaatc tatcaacatt
    36481 tcagtaataa cgttttctta attttcacta tggatgtcga ttcaattata attaaagctc
    36541 tacatcaatc tttttaccac tcattgaaca acaactaact tttgccttat caatagagaa
    36601 tatttcaaac ttaccatctc ctacagagta catctgacct aaacttggca ttatttcaag
    36661 tgccttagct tgtgtttctt ttgatgtaac aaatactgct tcacctaaaa tacgtagagt
    36721 attaaggttt tggtctatac aacaaagttc aacctttgga ttaaaaataa gttgcttaaa
    36781 catatccttt tgattagatg tgcaaaatgt aagtttacca ttgtattcca taacaaatcc
    36841 taaaggacgc acatgtgctt gattgttctc atcaactgtt gctaaataat aaactcgtgc
    36901 cttgttcaaa aattctaatg ctttcataat tcttacaccc cttttaaata cttgtattaa
    36961 ggattatcct ttgatataat tttaatataa aatacagaaa aaacaagtat gcagtttttt
    37021 ctgataaagt ataaaaaaga tactactata actaaggagt aattaaaatg agtataaata
    37081 ctgaaaaaaa tataactaaa aacgttgaag aattaacctg tccaatccgt tatgcattag
    37141 acattgtcgg tggtaagtgg aaattaccta ttatatgtat gttagcagtt gaaaatccaa
    37201 ttagatatag tagcataaaa agaaaattgg atggcattac aaatactatg ctagctcaat
    37261 cattaaaaga tttagagtct actggtattg ttcatcgtaa acaatataat gaaattcctc
    37321 caaaagttga atatacatta acaagtaaag gtaaaagtat tgtgcctatt ttacaacaat
    37381 ttgctaattg gggtgctact aatatgcaag aaaaaaacac atgtggactt agttgcaaag
    37441 aatgtagaaa gataaagtaa atttaaattt actaaattat tcatattata gactatatat
    37501 taatttatct gattgaatat aaaaacagga tgacttatat attcatcctg ttctatatgt
    37561 taagctctaa atttaattag ttcaagcata tcgtttaata ccaataaact atctacttga
    37621 atcattagcc atttacttcc attaaaaaag tttacctcat tatatatttt tttgatataa
    37681 tcacaacaag tattaataaa taaatcaacc tctactctat tgctttcact tacaataacc
    37741 aaagctttaa aataaccttc ttgtggatat aaagtacata gtgatttacc tcttttttta
    37801 tattttacat tccaacctct ttgcattgaa catttactgt attcaagctt aggttctaca
    37861 ttataagtag aaaccaaagt tttatttaac ttgtcccata atggattatt aataaattca
    37921 ttaatttgct caaatttagg tttagtatct tgaaaaaacg catcttgcca attcatattc
    37981 atcatccttt ctattgttca ataggaaccg gaacaatcca atgttccaga tatgctgctt
    38041 caggtgtagt ggggtaatac atctcaattg caaaatcagt agttgttaaa ttatgtttct
    38101 tcatccatct ttccataaat ttatctgctt taaaaacagc tgaattaact aactcattaa
    38161 agttttctgc ctcaaatcca catactaaat attctttagc tggcaactca aaagtactat
    38221 atccttctac tgatgtacct gactctacct ctacacctgc catatacata caatttcctt
    38281 cttttgcatt tcccatataa agagcagcaa attcatttcc acctggaatt tgatttggta
    38341 tattatgtct ttgacttcca agtttcatcc ataattcctc tactattgca attccagttg
    38401 tttcaccgcc caccaactgg caaataggta cttctatttc tattccaaca aatgtccttg
    38461 gattactcaa tgtcttacga ttgacttcaa tcactatatc atctgcaatc agtggaacac
    38521 cttcatcaac catagcataa tttaaaatta attctggctt aataaagtga ttgagtttta
    38581 ctggatttgc tctatatttt tctggagtta atccatatgc ttttttaaag gaacgtgtaa
    38641 atgtctcatg actagcaaat ccaaaattta aagctacatc aagtatacga ttcttatgtt
    38701 ctgctaagta ttcagaagct cttgcaagac gacgaagctt tatatactcc attacagtct
    38761 tatttaccaa tcttttaaat agtctttgat agtaaaatgg tgataaattt gctttatttg
    38821 ctaattcatt aattgatatt tcttctgata aatttgcttc tattaaatcc aatgtaattt
    38881 gaatagactc ccatgcatgc atactataac tcctcctgat atttttgtta ctcttatcct
    38941 ttatagtatc aaaactagaa aaaatgttct tgatagatag tgcccttttt aattttctta
    39001 tatgatagtg ctaaatctca ttgctttatt ataaacataa aatgtataaa aataaaatat
    39061 agcaaatctt taggtaagat ttgctatatt atatatttta ttaaactatt tttaaatact
    39121 ccatttattt ccttaagaaa gaactctttc atatcatcta attgttcttt atcatacacg
    39181 tacttaccat atccaaattg accgtattta aactttctat cctcattttc cattggcaat
    39241 agtgtatctg gataaatctc tagtattcta ttttttgctt tagttgtata tctatgagat
    39301 ataacttcaa atataatttt tttatcaaaa tcctttggta aagcttcttt tatacttata
    39361 atcagatttt tatactcttc tttccaacct tcatataaaa acacaggtgc tattatgaaa
    39421 ccaatctgat aaccagccct agcaagtttt acagctgact ctattctttt atttgcagag
    39481 gctgtataat gctcatgttc atttataatt ctaggagtat tgatactaaa tcgtatttct
    39541 gtatgattat tatgttcagc atccaataag ctatccacat cactgtattt agtaacaaat
    39601 ctaaatcttc ctttttcctc tcttccaaaa aactctattg ttttctttaa agaattagta
    39661 tatggctcta ctggaactgg gtctgatgta gctgcccctt caaatatggt tacctgtggc
    39721 tttctatctt caatatattt ttttgtctgt tcaagtattt catctacatt tacaaacacc
    39781 cttataaatg gtttatcacc taattgtgtg ttcaaataac aatactcaca tcttcccata
    39841 catccactga caagtggtag ttgataatta gcagaaggtt tacatgtttg aaacttcaga
    39901 ctttttctaa tcccaactac catagtctgc ttcccacttc tatattgttc atatagattt
    39961 tcacctggaa tatgttcttt aatcctattt gatgatatat taattatctc tacatcttta
    40021 ttatctctaa atttattgta tattttttta cccacatcag tgtctaaagt tcctttttcg
    40081 aatattattc ttttgggatt aaacatttat ttcacctcta cttaaagttt ttaaatcgaa
    40141 gaattttata aaatattact taa
//
LOCUS       DAGSRM010000030        39228 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.030, whole genome shotgun sequence.
ACCESSION   DAGSRM010000030 DAGSRM010000000
VERSION     DAGSRM010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 39228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39228
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.030"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(67..279)
                     /locus_tag="MJE33_001690"
     CDS             complement(67..279)
                     /locus_tag="MJE33_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY9647630.1"
                     /translation="MAILMKTKIKEYREKLLMTQNELAKLVGVRRETIVHLENGKYNP
                     SLKLAMDIAKVFDTTVENLFEFIEEE"
     gene            complement(280..696)
                     /locus_tag="MJE33_001691"
     CDS             complement(280..696)
                     /locus_tag="MJE33_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647631.1"
                     /translation="MSKSETGVRSKLILELLVITYFFIAVLATKYIFKGPSTASLPGL
                     SWLIVFGCYSIYNKKYNDLVDESSEFILSKVNKIAIKIMIYSIVIISIFFATPYTSNI
                     NISNLDTGIILIAVLFIQSLSKLVLYIYFDKRGICN"
     gene            complement(764..1177)
                     /locus_tag="MJE33_001692"
     CDS             complement(764..1177)
                     /locus_tag="MJE33_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647632.1"
                     /translation="MSKSELGVKTNLVSELLMISYFFIDSLLKKFIFKEGNLCITFII
                     LFAFWACDFMYNKKYNDLVDEASKLMLLKVDHIASKIMLYSILLIAVFFSANYTRGLD
                     ISNLDIGVILLAILFIQSLSKLILYIHFDRKGICN"
     gene            complement(2010..2639)
                     /locus_tag="MJE33_001693"
     CDS             complement(2010..2639)
                     /locus_tag="MJE33_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBY9647633.1"
                     /translation="MKNRKVYMYVFDTMSDWEVAYLTAELNTGRYFKKGLKPLKVITV
                     GIDKNPIITMGGLKVLPEITVDEFNMESKDLLILPGGNTWLSMNHEIILEKVKEALRQ
                     DSIVAAICGATLGLAKKGLLDFRNHTSNDLEFLKMVIPNYSGEKYYKTESVVSDENLV
                     TASGIAPLEFTFQVLKLLDVFESEALNSWLNLYKTHDSKYFFELMNCNK"
     gene            complement(2718..3680)
                     /locus_tag="MJE33_001694"
     CDS             complement(2718..3680)
                     /locus_tag="MJE33_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017561613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBY9647634.1"
                     /translation="MAKIDNMMSILWMLNSDKKITAKQIAEKLEINIRTVYRYIDVLS
                     ASGVPIISDTGHNGGYSLLNNFTKAPLFFDIEEQKSLLHAALFAKKEGYFLDEALDKS
                     ILKLKMYSNQKQEDILTNHLRALEVIKPIGKVSIESVLKKLENSILKELSVEINYHAG
                     RKVKSKYRIINPYGIVHWNNNWYVVAFCNMRNEVRSFRVDRISEIIETSNTFNRPTEF
                     SASEFFIKGIIPEIKDEQNIIQLVIEGSIDSLDSLSQHWFLGHYLKERTPNKIIFMLE
                     EEVICKYIPNILLPYRKTIQVIEPLSLRKKIIEDLLELIDYYQI"
     gene            complement(4442..8038)
                     /gene="cbpA"
                     /locus_tag="MJE33_001695"
     CDS             complement(4442..8038)
                     /gene="cbpA"
                     /locus_tag="MJE33_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding adhesin CbpA"
                     /protein_id="HBY9647635.1"
                     /translation="MQQKCKKKALSMILSISMGLGSVASFNASNTFADTIEESTNAGN
                     AEGDTNAGNAGGDANAGNAEGDANAGNAGGDTNVGNPEGDANAGNAGGDANAGNAEED
                     ANAGNAEEDANAGNAEGDANAGNAEEDSNAGNAEEDSNAGNAEGDMNALVVEEATAED
                     PTSVGDFKISGGEINVDFKFASGVLTILSGKQMTIQNNNPNTPTTHRIVIPKDVKAEL
                     IFDGVNIKTASNSPFTLTPDKDGGAYAHVILQDGSENKLESGDVYYPGMRAGKGTTLI
                     IDDSVVNRDIEGEFIVSEGGKIPRDVTLENGDKLKKGDRLTLLDSNNRGSLKVVGNYY
                     SAGIGGGNTESGGNIIINGGAITAIGQESSRNTGYGAGIGGGGNGSGGDITINGGTIT
                     AKGAYHGAGIGGGYYTTTKNALSPSVVDPSTGSGYSGNITINGGLSYSYGGAHGSAFG
                     DGCVNPGTNRGYRILVTGGTILPYMTGVGDWIFDLSATTGDVIVTGGSLKATTFKSLG
                     GAVAYGDLEKKTKVFMNKISLTYLGQDKVSTTLVDDFKMLIDGKIYPYGAPSYTDAQG
                     ILYLWFSDKFTGSEVSVNLVVIDKITGEVLKPDDFYIKDIGSGSQFLKQYEEFTIDDT
                     PPYLIIKRYDGLPFEEDVEKSFLDWLLKDGGIPITTPKGEKLTDKKFMTISSQRLNEE
                     TMEPDINLGSDDLTKQVDAGKYQLSITSTQFSSIYPFKDAYWGHRAYYKAEIIPADTE
                     TSLVVEESKAKTDVFKPTDTFTLNVTISPDKKEGPDCASPRGYVQFYINGKEYKDPVE
                     LKTHNKGSESHNYSTASIEWRPLDSNHHIFTTEQTITVKYIGDDINYIEGSEDEKKLK
                     LDMTNVDVDGDGIPDINIDTDGDGRPDINIDTSGDWKPDINIDTDNTGEWKPSTEGGN
                     GDGIWKPDKNIDTNGDGNPDTDYNRPAIDTDNDGVDDYWKPDKNVDTGSGGYDTGNPN
                     LNKPDPDNGGNSKPDSGNGGNSKPDSGNGGNSKPDSGNGGNSKPDSGNGGNSKPDPDN
                     GGNSKPDPDNGGNGKPDPDNGGNSKPDPDNGGNSKPDPDNGGNSKPDIDTDGDGKPNI
                     NIDTDGDGKPDINIDTDGDGKPDINIDTDGDGKPDINIDIDGDGKPDINIDTDGNGKP
                     DINIDTDESLDSNVGQNVQTGDQSNIMLDLALMFISLFFLIKNLTNKYLRRK"
     gene            8307..8879
                     /locus_tag="MJE33_001696"
     CDS             8307..8879
                     /locus_tag="MJE33_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HBY9647636.1"
                     /translation="MKKLVKILINIICIITLIFSSLSIYIKLSEYKKADEVYTELREN
                     TINNSKYQELYDKNNDYRFWLKINNVNIDYPVVQGYNNDFYLTHDFYKNYLPLGSIFM
                     DYRNNFENDKSLIVYGHYMKNKTMFGQLENYTDEVFFKENNLVEINYKVQTYTYEIFS
                     VYTADLINRDYLSIHFNNNDEFKYSLNYIT"
     gene            complement(9892..10554)
                     /locus_tag="MJE33_001697"
     CDS             complement(9892..10554)
                     /locus_tag="MJE33_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9647637.1"
                     /translation="MKMLKVIDIKKIYGKGENEIKAVDGITLEIEPHKFSAIIGQSGS
                     GKSTLLHCMAGLDKPTLGNVFMDNLDLYTLSDEKLSNLRSKEFGFIFQNYNLIPVINV
                     YDNIVLPILIAKGKIDNNYINDLITKLGLESQINKFPNELSGGQQQRVAIARSLANKP
                     SIIFADEPTGNLDSKTTNEVMNLLKYCVNEYKQTLVMITHNDEIAKMADCIITISDGK
                     VI"
     gene            complement(10571..13125)
                     /locus_tag="MJE33_001698"
                     /pseudo
     CDS             complement(10571..13125)
                     /locus_tag="MJE33_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433803.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
     gene            complement(13358..13552)
                     /locus_tag="MJE33_001699"
     CDS             complement(13358..13552)
                     /locus_tag="MJE33_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HBY9647638.1"
                     /translation="MRKYKAILLTLIISIIAIIGFIIYNNYFRKWNEAEKYIDKYMTY
                     QGVSGDDIERITKEKYTKEN"
     gene            complement(13564..13743)
                     /locus_tag="MJE33_001700"
     CDS             complement(13564..13743)
                     /locus_tag="MJE33_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647639.1"
                     /translation="MIHVEISEKEFFRKIEEVFDKGEGNIEVDNLGDIKIQVKKFDDK
                     TKSLELKLIKEFKLK"
     gene            complement(13748..14005)
                     /locus_tag="MJE33_001701"
     CDS             complement(13748..14005)
                     /locus_tag="MJE33_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647640.1"
                     /translation="MSKKISESIENSGLRVAENKSDGSFVLSLGNLEYTKEQPSQVLH
                     YSMTKDKKGTKEILSIQCVKDFSYNEKLSEDDKFIMAIYNI"
     gene            complement(14265..15658)
                     /locus_tag="MJE33_001702"
                     /pseudo
     CDS             complement(14265..15658)
                     /locus_tag="MJE33_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            complement(15652..15897)
                     /locus_tag="MJE33_001703"
     CDS             complement(15652..15897)
                     /locus_tag="MJE33_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HBY9647641.1"
                     /translation="MLIHTKNKTFDAICILEKIEKDLNIEELVRCHKSYIINLNYVEN
                     IKSNTAILDSQEEVPISRYRYKDIKERFLKFIGDRIC"
     gene            complement(16214..16366)
                     /locus_tag="MJE33_001704"
     CDS             complement(16214..16366)
                     /locus_tag="MJE33_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647642.1"
                     /translation="MKNLNKNTILGEVIITIAACDDEKEIQIVIKNQVDNILKNKNKE
                     YRIKKI"
     gene            16975..18441
                     /gene="dndC"
                     /locus_tag="MJE33_001705"
     CDS             16975..18441
                     /gene="dndC"
                     /locus_tag="MJE33_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA phosphorothioation system sulfurtransferase
                     DndC"
                     /protein_id="HBY9647643.1"
                     /translation="MAVNYLDNNNLENLYDEMQAVYLNDDRPWIIGYSSGKDSSCVVE
                     LTFRMLKRLPKEKRHKEIYVISSDTLIENPIILDYLKNNIDKINESATEHDLPISAQI
                     VYPELNDSFWANIIGRGYPTPKSIKYRWCTERLKIKPSNKFIKEKLEQKDVIVLLGVR
                     KEESSARKSRIEKREIEGYLLTPHETLRGKNKLAYVYTPIVNFSTADVWDVLYHQNDN
                     FIDFGGEFGPSPSTSWGTDAMELFQMYMKGSGDSDECPFIADEASAKSSCGNSRFGCW
                     ICTVVKEDKSLNGFIESGHDELKPLLEFRSWLISERDKPKNRKKHKRNGSVIKKDGRI
                     MFGPFTFEARQTILRKLLETQLEMQKFYPDLQLIQLGELKAIDDIWDNEEDLTGNILS
                     KIYKEVVGKNLPWSEYKKPVFDDLTLNIISEKCSEYDINIDLFNKLMIDTNKYKYFSN
                     NTKLKTSIERILNQQWLHQDIIEKIEEESNKELKYENK"
     gene            18428..20587
                     /locus_tag="MJE33_001706"
     CDS             18428..20587
                     /locus_tag="MJE33_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBY9647644.1"
                     /translation="MKINKLVLKNFRSYEEETTFNLNTTSEKNIILIGGKNGAGKSTI
                     FEAIKLCIYGPIAYKYQGFNSSYISRVKSNINNNSLRNSKVDTLVSVDIEISEGTEKN
                     TYTLIRQWTFKDKKLNETFSVYKNFSFTPLNADELNYFENYITSIISPKIFDFFFFDG
                     EHLYDFFIGKNSNIHLKESLLSLCNFDTFDILKKMLISTNRSNKNVSDEINIIKDDYL
                     NLEVEINNLNSQEKNLSYELEKISSEIEKLQQEQLKIKKEFRKKGGILAEEREMLNSR
                     IAELEGNRFIINQNIKDFCNDILPFLIVKKQILNLEKQLKVENDSILYSHLKDKLNIE
                     YIKNLLSDKIMSSSLDEIALTVSEALIEDIKPDSYEDNFESIHKLSNDESSKLLSLIN
                     TILNFDNNSIINSFGEHRSISNELADIRHKLNTSFKDETLNTYINDISSLSDKIDSLL
                     KSKNLLVTSLDKLRLEIAETITKRDKSKAKYIYLLQHNNIVDISSNLILMLDEIITTL
                     TETKIKLVQDNFMYIFKRIIQKDNFISSIDIDANFNISLYINKIYNSLELENLVENIG
                     SEEMEKKLGKLFFEDLFNLYRVKDKDDLFNAIKNNTQSTFLNLRTKVDIDEFSSGEKQ
                     IYILCLYWALLKSSEIEIPFIIDTPYARIDDTHRNNITSEYFSTISDQAIILSTNTEI
                     DEKSYKIIKPKLSNEYLIEYDNKNKKTIQNKGYFFEV"
     gene            20590..20973
                     /locus_tag="MJE33_001707"
     CDS             20590..20973
                     /locus_tag="MJE33_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DndE family protein"
                     /protein_id="HBY9647645.1"
                     /translation="MGFRLKTSIQTKEIFEELGSRMNLKPFALCKLAISISLSNSEPI
                     ENYESIDNNGLELNRQTITNQYDTLFKTLLEQHAGRHLSDDEYFPNQVKLHIDRGALM
                     LKNRYNYSSNLEHFITQIVQENNNL"
     gene            complement(21083..22225)
                     /locus_tag="MJE33_001708"
     CDS             complement(21083..22225)
                     /locus_tag="MJE33_001708"
                     /inference="COORDINATES: protein motif:HMM:NF014044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBY9647646.1"
                     /translation="MKIIFGNIKNNNKELIWDPTNSIKVPHPNTGIIGTMGTGKTQFT
                     KSLVTQIHREQKHNINGKPIGILIFDYKGDYIKDDFVDATSAKVYEIYHLPYNPLTLI
                     IPERIKPLLPVHTANSLKETIAKAFGLGPKQEVILRELIMKAYEKKGIKKDDENTWVK
                     SCPTMQDVLSIYLDDREIKKDDKLYAALSSFNDFEIFEPNSEKTKNLFDLINGVTVIN
                     LSYYDSGIQNLVVAITLDLFYSQMKALGSSDITGNGEYRQLNKMILVDEADNFLSRNF
                     TSIKNILKEGREFGVGTILSTQLLSHFSTSENEYQNYISTWIIHNVSDINNKDIRYIF
                     NSKNKSEEDNIYNKIKNLAKHESLVKMPSENSPIYIRDKAFWELNK"
     gene            complement(22235..26206)
                     /gene="dptH"
                     /locus_tag="MJE33_001709"
     CDS             complement(22235..26206)
                     /gene="dptH"
                     /locus_tag="MJE33_001709"
                     /inference="COORDINATES: protein motif:HMM:TIGR03237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA phosphorothioation-dependent restriction
                     protein DptH"
                     /protein_id="HBY9647647.1"
                     /translation="MSDQFYRYLAKNLVKYFESREIKSGDKFNIQFEREEQVKIMYDV
                     LEKESNSRPFTYNIMNGSKYQTFSMNVSGIQLIVASNNNTTNDFLIRLRNLIGNVASD
                     EFKDSAILFIHNMTLDSLIRGTESLQKEGMPFHTKTLVKDIQNKLENDKLKEDIKHIL
                     YFVLDRMNKSILEDNSSIFQYENILDILNKGRIEKSEYSKFGLFYDDNMLDNKSSKAI
                     KERLLDNEKLFSIVNSIHQYGDPKLELEKHFDSKGIDLLKDENWKYSTYKDIKSSQER
                     MKNKKVELLCIECVENLKLWSKPDIENKSKRKNILLFNKDKLDIINLCIKFDKKIEKT
                     SFSIDKMSKTYTEVKASGNKAYISININISETTFTKVKYNDGIIKYEFRICIVSCDEN
                     ILNDIKSNYGIVINSKRSMLVTNTDDDTIVINPKGIIERKIQIDEFENEINLEEGERL
                     DLELNVELEEDVEFIKIDVVYDQSVITIVKQEEKEQPISISGMKTWKLKRERKESFKY
                     LGNSKIVQGTSEYFPKENFKENLQIEKYIIENNIMHCTILEEKYKIIELDVDSRIKEA
                     YKNLISYYKVNDCIPSLAYYDVDLVNISKNYINTILEVFEDIKNGKVLSKERLNIAKV
                     GTIHRIDKEEELFLTPLHPVNVAYQLMINNVIGDEEVKEGILKLLGSKYLMPYIYKGK
                     NSLYKVVDQRHSPEWTYYVDHDMSRYKGSRDYVSKLTKEKINEFIEHFTYLFRDISKS
                     VMKIKLINLGDCKEVLLGIFDYYIKKLGKGEEINNLISIDIYIYGDKSIINSFEEIAF
                     YNSINDIKKHFNVNLSVDNYSEEDVLNLFRDKVKFYKKDKMSDLYEYSHLTLYQMDKD
                     VNITSSSMDDIKTGVALNGLIAGVPSIYLEDTYRTGFGSKFMATTKNELLNISMKFNS
                     LTKSIGTGDPFNDNLSVTTSISENNSSFLNKIYKTSHWVTFIDPKVDLNFFKDDENSN
                     DLLIIHYSDQYTTSSGYDAITVTRKSEQYKFIIEEFLNSKDIKVEEDTIPNLINYFNA
                     INGDWLLRLISSKSQFPREKLSILSAIKISLAYFYHPNIIWIPISLEEILRVSGGAGL
                     KQAGGLFTVKKLTGDTGSYSDDLLLVGIENKNNKIKVYFYPVEVKIGDNNESVIKKAI
                     IQANKTRDLIEEYLTESENDSQKFTKLMYRNFMIQLAIVSAQKMKLYHIWDEQNWDDI
                     INSDIRTNLLNDNYEISNDLDEFIGRGAVISFKKGTYYNGNLGKEGNITILNLSEQEG
                     YNNIVMDLEEMKEKYIRGESDFVIQEMLINKYLDNTIGIQNMDKNVILDTNVMLNKTK
                     DYD"
     gene            complement(26187..27608)
                     /gene="dptG"
                     /locus_tag="MJE33_001710"
     CDS             complement(26187..27608)
                     /gene="dptG"
                     /locus_tag="MJE33_001710"
                     /inference="COORDINATES: protein motif:HMM:TIGR03236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA phosphorothioation-dependent restriction
                     protein DptG"
                     /protein_id="HBY9647648.1"
                     /translation="MQLIINKEYFKQNYMIDKGIARHNSVKNIVILPYTTTTKEKSKL
                     KEKVERFESCVGEFCRLVYNKKMNIKERQLKDIKLLIKDINVNSSDDSVQLESIIREL
                     FLDEEENMYIFHPKLLNYIHMPSDSYYKKLSKFLYDVLLNSTDNITIKNKINKCFEYE
                     PDNIMELIILKNLPELENEETSKSIYKCICKNVSKLFQEDLEFMLNYPELIINEFERL
                     LKYYYFFYVSQLSIKLSHFFDGDRDRTEKVYFILSWEKASKTRISYNMGWKMLESHIR
                     ILFSHVHTLEILNTNNDISKYDYVDLNKIIKNLSENERLRLYENIIDIKNTYVKSITD
                     VQWDDYDEQTKVYKNDNIKEEICDLHKRIDYQFKKSSREANYKRYKNLFEEYCKLNFL
                     KKAGPLGNVLKINQEELLFMTKLCMKNKTKIKLKELFEEYEKRGIYFDRDSQSKVVEL
                     FEKLNIIEKKSDSGDAQYVRSIL"
     gene            complement(27596..29404)
                     /gene="dptF"
                     /locus_tag="MJE33_001711"
     CDS             complement(27596..29404)
                     /gene="dptF"
                     /locus_tag="MJE33_001711"
                     /inference="COORDINATES: protein motif:HMM:TIGR03238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA phosphorothioation-dependent restriction
                     protein DptF"
                     /protein_id="HBY9647649.1"
                     /translation="MSNGCLIDELKKLKESSKEAVENISDFSSFKKYMHVKRQVENEL
                     LDIIKYTADSNKAELILVCGGVGDGKSHLISYLNNKYPEIMSKFIIHNDATESFEPNK
                     TSIDTLNDVLDDFSDKKMDNSDKKLVLAINLGALSNFLESEYVNKFTKLREFVENKGI
                     LDISISDDDDKNSNFHFVNFSDYHIYTLNADKPKSRYIESIINKIIAEDNQNIFINSY
                     KTNCTHCIVNYKCPIKNNYEMLMRDSVRETLVDILIESTVKYKIIISTRSLLNFIYDF
                     IVNVDLDNKNNKDLIKFVEELSYEAFIKYIFVSNLYNHSELSQILNAVNKIDPINITS
                     ENLDNIIIKLSNTKDMTILFNQYLNLSKNSYLESSMTNREIIESALNSSKNELSRKIL
                     KYNIINTFIRLHIFIPKNNLQLRDNIYISYMRNLYYFNKGEKTYLSNLYDDIKEALYS
                     WNGRIQDDRINMSIGKNQMRYKISQRLNIIPDLSKLRKCTEEELHKFKPSLEIGYKND
                     EKENQDTCTVDIDFNLYKLLMRVKYGYTPNNNDKYTYINFVVFMNKIHKLGNQNRELY
                     IEDRLEDTVPKYKLTYDKEFDKFKFIRVNGVKVCNL"
     gene            complement(29821..30093)
                     /locus_tag="MJE33_001712"
     CDS             complement(29821..30093)
                     /locus_tag="MJE33_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647650.1"
                     /translation="MYDEIIEIYFDYWINEHMYRDILEKDKYYIESWKEVKKINELLE
                     KVLEDDKKYVLVELNDLFNSLIEFERELSYKLGFKTGAKFIIELMD"
     gene            complement(30341..31675)
                     /locus_tag="MJE33_001713"
     CDS             complement(30341..31675)
                     /locus_tag="MJE33_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBY9647651.1"
                     /translation="MNNSNNLIKNTEAEQSILGSILLDSTIVQELEQMLNEDMFYHEE
                     HKYIFNAIKEINKRNEVIDIITLTEELKRKGHLNNIGGISYITSLSTIVPTVSNFMSY
                     VSIVIEKYKNRNIIDKFKKFNLGEIDDNTLIYQLENDINKANQKLIKKDSSITAISER
                     AFEYINDEVNEGIKTGIKFLDDTIGGLYGGELTTIAAKSGRGKTALALQILRNVIFQG
                     KKVLFISGEMSDIQILFRNIASLTGISVVKMKNRNLNDEEIVKYIQALSIINQENNLY
                     INDSVNTISGIKREIKSIKPSLVIIDYLQILDYEGKEVGEAKFADLSRQIKKITLDFN
                     IPVIQLAQLNDELKDERPKGDRVMRSSKQIYMDSNSVIYIHRPTEKEALNYCKTMGIE
                     DIRELRRREKGFGANLSEIIVAKNRDGESGFRPYWYIGNLFSFEECKKIQEI"
     gene            complement(31688..32431)
                     /locus_tag="MJE33_001714"
     CDS             complement(31688..32431)
                     /locus_tag="MJE33_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647652.1"
                     /translation="MSNKGWIKLYRELLDKSIWKDIKPERKVILITLLLMASHKENHV
                     FIDNKRIKIKSGQFISSYSKIAERCGKGVSYQMVRSCLLVFKNAGFLTYETTNKYIVI
                     TIENWELYQENIENQQPNQQTINNQSTTIKNIKNENNIIYSEIISKFNSTCIELSKVK
                     KLTDTRKRRINSRIKEYDKETVLEVINIVSKTSFLNGNNNTGWKANFDWIMNSSNFTK
                     ILEGNYNSKEIVINKETGIYNPDDSQLKW"
     gene            complement(32453..32767)
                     /locus_tag="MJE33_001715"
     CDS             complement(32453..32767)
                     /locus_tag="MJE33_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647653.1"
                     /translation="MKENNNLEVLKTLQATLNIIVKNEEEAQEKKQGYKIIENAKRNK
                     AELITCKEASKLYPIGESKFRQLCHSKNKGFPCIWINNRVYIIKNKLDDWFIENANGI
                     KL"
     gene            complement(32771..32971)
                     /locus_tag="MJE33_001716"
     CDS             complement(32771..32971)
                     /locus_tag="MJE33_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY9647654.1"
                     /translation="MGINLKIFRIKKGLKQQELADRVGISRYYLSNLETGKANNPSND
                     LMIKLSRALDTTVEELFFSKED"
     gene            33118..33882
                     /locus_tag="MJE33_001717"
     CDS             33118..33882
                     /locus_tag="MJE33_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY9647655.1"
                     /translation="MQIGKNLKNIRKQKELTQIQLAEISGISRNALINYENDKRIPSI
                     DTLSKLAKALKIEKTIFYFDIENEVMTNEDVLYLLQSLFPEEYYSFDEFLNAISNLME
                     DDDIRITSSLLLGAWSDKDQYYKLGEKLELNENQVYNWILSDCISNTELDFSLHPDDI
                     KEIIKNDVLTKQSIDELLDIGLSEENIETIYDYFSDSTDNGSNIRLKKLISQKENITI
                     KNNDYFKLLQENNELKEKNEKLENTLKEIKKLLTKI"
     gene            33939..34137
                     /locus_tag="MJE33_001718"
                     /pseudo
     CDS             33939..34137
                     /locus_tag="MJE33_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729178.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            34134..34625
                     /locus_tag="MJE33_001719"
     CDS             34134..34625
                     /locus_tag="MJE33_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY9647656.1"
                     /translation="MMNNTIGEKIKQYRKSINMSRDELANKIGCSVHAIAKYEQNQRM
                     PSLDMIRKIATALSIAINKLIVEENDLDTLTNNNINLKVFDKELDLVIHILENYGYDI
                     KTDYKKKFEITDNADTIKPINLNFSDFKDFSKNLNWVINGLVENFIQENSNGLIEDED
                     EEY"
     gene            34717..35469
                     /locus_tag="MJE33_001720"
     CDS             34717..35469
                     /locus_tag="MJE33_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBY9647657.1"
                     /translation="MNKSYIPKYTHKFPTNLPHGDKKYTLTYIRRMISEFVYDMGNLE
                     NNPFTFPEVQTLLDGITVGGHKLSDQNQILNIKSSWDYVIKLSNKENLSLNKDTICSI
                     HKIVAKDEALIVGDFRNGNIGISGTTQYECIEATLLNDLFVNDINIINKITNPLEKAI
                     IINLWLSYCQFFYDGNKRTARLTSNLILISNDIGVLSIPAKHKQEYNTLMLNFYETLE
                     ADEVIKFLLEKCITFFDGYNYKSYKELFKNGN"
     gene            35459..36625
                     /locus_tag="MJE33_001721"
     CDS             35459..36625
                     /locus_tag="MJE33_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBY9647658.1"
                     /translation="MATKRANGEGSIGKYKDGWRSRIMIGYNEDGKPIRKEFYGKTQK
                     EVKDKLDAYKKQYYLSSDISDDKITLEHWFYNWLFEYRIKDLKPKSFERYESIYRNYI
                     KDTDLGNVKLKDLRVSHIQKYYNRLLDSNKSIPTIRQINTKLKTCLSEAEKQGLIQRN
                     YCTMVNIPVEKKENKLEILTLEQQKSFIKAIDGHKLEVLFLLALGTGLRLGELLGLKW
                     FDIDFKKSNLTVKRTLQRTYFIDKTGNRELKVLEQEPKTSNSYRTVPIPKDVFNKLKE
                     HKVNQNSDILKAGELYRNDNYVFCNELGIPIDDKRPLRNLKSILNSLDIEPIKFHGLR
                     KTYATRLFENDVPPKTVQVLMGHYDISITLNIYTQVMEDKKVEAVEKLDKIFSL"
     gene            complement(36741..36971)
                     /locus_tag="MJE33_001722"
     CDS             complement(36741..36971)
                     /locus_tag="MJE33_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647659.1"
                     /translation="MEANKTLLQILYKKIILEFSKQTGKSLEESMDYLYTSETYKLIS
                     EGVADMHCKGHIYLAQELMLENGLMYHKGYPR"
     gene            complement(36977..37456)
                     /locus_tag="MJE33_001723"
     CDS             complement(36977..37456)
                     /locus_tag="MJE33_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HBY9647660.1"
                     /translation="MIVYHGSYCVVKTPSITFSRDSLDFGKGFYVTSIKTQAINWTER
                     FKKRGKKAYLNSYSLDIDKLKENCKVKIFNSYNLEWLDFIIECRNMSDIYMKYDIIIG
                     GIADDKIYNTIELYQDNLIEKDEALKRLKYYKPNEQICITNQNVIDKYLKFRYEEEV"
     gene            complement(37456..37671)
                     /locus_tag="MJE33_001724"
     CDS             complement(37456..37671)
                     /locus_tag="MJE33_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HBY9647661.1"
                     /translation="MDERKLEFSIFCIEALAEKLNIDSEGVYKLLKHDTDILDNYIIP
                     CYEALHSQSKGYIVEDLMQVLKEKGVI"
     gene            complement(37987..38336)
                     /gene="ssrA"
                     /locus_tag="MJE33_001725"
     tmRNA           complement(37987..38336)
                     /gene="ssrA"
                     /locus_tag="MJE33_001725"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(38494..38949)
                     /gene="smpB"
                     /locus_tag="MJE33_001726"
     CDS             complement(38494..38949)
                     /gene="smpB"
                     /locus_tag="MJE33_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBY9647662.1"
                     /translation="MAGKKILATNKRARHEYFIEETYECGIELKGTEVKSIRQGKVNL
                     SDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIRKLIGATTIKGYS
                     LIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMTGKY"
BASE COUNT        13411 a         6024 c         4156 g        15637 t
ORIGIN      
        1 taacaaactt aataattaaa taactatatt ttattatcca tcttataaat gtttataaac
       61 tttccactat tcttcttcaa taaattcaaa caaattttct actgtagtat cgaatacttt
      121 agcgatatcc atagctaact tcaaagaagg attatatttc ccattttcta agtgtacaat
      181 agtctcacgt cttaccccaa ccaatttagc aagttcattt tgcgtcataa ggagtttttc
      241 tctgtattct ttaatttttg ttttcatcaa tattgccact tagttacata tccccctttt
      301 atcaaagtat atatacaaca ctaatttaga taaagactgt ataaataaaa ctgcaatgag
      361 gattatacct gtatctaggt ttgatatatt tatattactt gtataaggtg ttgcaaagaa
      421 aatagatatt attactatag aatatatcat tatttttatt gcaatcttat taacctttga
      481 tagtataaat tcagatgatt catcaactaa atcattatat tttttattat aaattgaata
      541 acatccaaaa actattaacc aactcaaccc tggaagagaa gcagtgcttg gacctttaaa
      601 aatatactta gtggctaata cagctataaa aaaataagtt attacaagca actccaggat
      661 aagctttgat cttacaccag tttctgattt agacatataa aatcactcct taaaaattat
      721 attctctata ctctttaatt ttcgttttta tcaatattat cacttagttg catattccct
      781 ttctatcaaa atgtatatac aaaattaatt tagataaaga ttgtataaat aaaatagcta
      841 agagaattac acctatatcc aaatttgata tatccaaacc tcttgtataa tttgctgaaa
      901 agaaaacagc tattagcaaa atagaatata acattatttt tgatgcaata tgatcaacct
      961 ttaataacat aagttttgac gcctcgtcaa ctaaatcatt atatttttta ttatacatga
     1021 agtcacatgc ccaaaaagcg aataaaatta taaaagtaat acaaagattt ccctctttaa
     1081 aaataaactt ttttaataat gagtctataa aaaaatagga tatcataagt aactctgaaa
     1141 caagatttgt tttaacacca agctctgatt tagacatata aaatcacccc ttaaaagtta
     1201 tatatttata acaccttatg ttataaatat ataacttttc caaataaatt gcaatagaat
     1261 attgaaaata ttcaataata tcttcataaa tatattttat aaaagatttt ataataaaca
     1321 ttacaataca gtactaaatt agtttatttc atctatcatc tcaatcatat catttactaa
     1381 ctattattct aatgagcata taacattgaa acttataatt atacaaccta aatgcctccc
     1441 aatgtaccct caaaacatca tttcctgtaa ctgaagtata attggtatat tttatcactg
     1501 aaataaaaaa acactagtca ataaagacta gtattttcaa gtattttatc attctttttt
     1561 taattattgg agacgaatca tcagaataat gcccataatt tatctatatg caaattctaa
     1621 atttaaaata aagtttcaga taaatttact attctatgct atgatacaac agaaaaaact
     1681 gaaatctatg agtggtagct tcatataatc ctagttccta cagtaatcaa aactgaggtt
     1741 tattctatat gtaactaatg ataaatttta ttataggagt tattagatct atttgtgtta
     1801 ctataagtag taattatata tatgataatt ttttaacact tttctgttgg ctatatagaa
     1861 agtggtttag aatttaaatt taaataaaat ttaaattcac caaaaagaaa tctatattag
     1921 aaaccactac tctattccac cagattgtaa ttttttgttc tttatatcac aatgctatac
     1981 catattaaat tgaaaaataa gttttaaatt tatttgttac aattcattaa ttcaaaaaaa
     2041 tattttgagt catgagtttt gtaaagattt agccatgaat ttaatgcttc tgattcaaaa
     2101 acatctaaaa gtttcaatac ttgaaacgta aattccaacg gagcaattcc tgaagccgta
     2161 actaaatttt catcactaac tacagattcc gttttataat atttttctcc agagtaattt
     2221 ggtattacca ttttaagaaa ctctaagtca ttacttgtat gatttctaaa atctaatagc
     2281 ccttttttag ctaggcctaa tgtagctcca catatggctg caactataga atcttgtctt
     2341 aaagcttctt taactttttc taatattatc tcatgattca tagataacca agtatttccc
     2401 cctggcaaaa ttaataaatc cttactttcc atattaaact catcaactgt aatttcaggt
     2461 aaaactttta acccacccat tgttataata ggatttttat caattcctac agtaattact
     2521 tttagtggct ttaatccttt tttaaaatat cttcctgtat ttaattcagc agttaaatat
     2581 gccacttccc aatctgacat agtatcaaat acatacatat atacttttct atttttcatc
     2641 ttttttactc cttatgtaat taatttatat atctaattat aaaataactt aactgacata
     2701 ttcagtcagt taagctatta aatttgataa taatcaatta attctagtaa atcttcaatt
     2761 attttcttcc taaggcttag tggttcaatt acttgtattg tctttctata aggtaaaagt
     2821 atatttggta tgtacttgca tattacttcc tcttcaagca taaatattat tttattaggt
     2881 gttctttctt tcaagtagtg ccctaaaaac caatgttggc ttaaactatc taaagaatct
     2941 atactccctt ctataactaa ttgaattata ttttgttcat cttttatttc aggtataatt
     3001 ccttttataa aaaattcact tgctgaaaat tctgtcggtc tgttaaatgt atttgatgtt
     3061 tctatgattt cactaatcct atccactctg aaactacgaa cctcatttct catattacaa
     3121 aatgcaacta cgtaccaatt attattccag tgaacaattc catatggatt tataattcta
     3181 tattttgatt ttactttacg acctgcatga tagttgattt ctacagatag ctcctttaat
     3241 atactattct ccaatttttt aagtactgat tctatagaca ctttcccaat tggttttatt
     3301 acttcaaggg ctcttaagtg gtttgttaaa atatcttctt gtttttgatt tgaatacatt
     3361 tttaacttta atatcgattt atctaatgct tcgtctaaga agtatccttc ttttttagca
     3421 aatagtgctg catgaagtag agatttttgc tcttctatat caaaaaaaag aggtgcttta
     3481 gtaaaattat ttaaaaggct atacccacca ttatgacctg tatctgaaat tataggtact
     3541 ccactcgcag aaagtacatc aatatatcgg tacactgttc ttatatttat ttctaatttt
     3601 tcagctatct gttttgctgt aatttttttg tcagagttta acatccacag tatagacatc
     3661 atattgtcaa ttttagccat gtagtttcac ctctttctat ttgttatata aagttattat
     3721 ataacttttt gattgatttt ttacaaaaaa acattagtaa actgctaata atttatacta
     3781 agatttattt caaatattta tagatagtct ttttctatag ttaagttatc cattaaatta
     3841 catagtagta tatctatttt agttgatata ttttcagcta cctcccttga atattattac
     3901 aataagtcta aatcttcttt cttccataaa tatattatga ggaaagacta tcatctttaa
     3961 tggtttaata gtataaaaga tattaattta aaaaaatcta tttttatatc agatgggtat
     4021 tgttttttat aaaactgaaa aaatcaccta atagaatcta tgatttttta acataacctc
     4081 cataaattag ttccaattat cacttcaaat tcaaattttt atctgaagaa tatacaatca
     4141 tatttccata ctcaacataa tattgagtgt ttggcttgta tttacaaatt attattctaa
     4201 caaaaacaac tatacataga ttaaaatcat cttctaaaca taactactaa aattgaatta
     4261 atgatatcaa ttccattaat ctcttttaat aaaaaattct cttttctata tgaaaattat
     4321 ttcaaaactt ttaagttaaa tacaaaaatg actataaata gatttttatt tacacaaatc
     4381 tatttatagt cactatagtt tatccaataa aataataaaa taaatatgta cacaataaat
     4441 gttatttacg tcttaagtat ttatttgtta gatttttaat taaaaaaaac aagctaatga
     4501 acattaatgc taaatctagc attatattgc tttgatctcc tgtttgtaca ttttgaccta
     4561 cattactatc taaactttca tctgtatcta tatttatatc tggttttcca ttcccatctg
     4621 tatctatatt tatatccggt tttccatctc catctatatc tatatttata tctggttttc
     4681 catctccatc tgtatctata tttatatctg gttttccatc tccatctgtg tctatattta
     4741 tatctggttt tccatctcca tctgtgtcta tatttatatt cggttttcca tccccatctg
     4801 tatctatatc tggtttgcta tttcctccat tatctgggtc tggtttacta tttccaccgt
     4861 tatctggatc cggtttgcta tttcctccat tatctggatc tggtttacca ttgccaccgt
     4921 tatctggatc tggtttgcta tttcctccgt tatctggatc cggtttgcta tttcctccat
     4981 tacctgaatc cggtttgcta tttcctccat tacctgaatc cggtttgcta tttcctccat
     5041 tacctgaatc tggtttgcta tttcctccat tacctgaatc tggtttgcta tttcctccat
     5101 tatctggatc tggcttattt agatttggat tacctgtatc ataacctcct gagccagtat
     5161 ctacattttt atcaggcttc caataatcat ctactccatc attatctgta tctattgctg
     5221 gacgattata atctgtatca gggtttccat ctccattggt atctatattt ttatcaggtt
     5281 tccaaatacc atctccattt ccaccttctg tactaggctt ccattcacct gtattatcag
     5341 tatctatgtt aatatctggc ttccaatcac cactcgtatc tatattgata tctggccttc
     5401 catcaccatc agtgtctata tttatatccg gtattccatc cccatctaca tctacatttg
     5461 tcatatctaa ctttaacttc ttttcatcct cactaccttc aatgtaattt atatcatctc
     5521 caatatattt tacagtaata gtttgttcag tagtaaaaat atgatgattg ctatctaaag
     5581 gtctccattc aatagatgca gtactataat tatgactctc actaccctta ttatgagttt
     5641 ttaattctac tggatcttta tattcctttc cattaatata gaattgtacg taacctcttg
     5701 gagaggcaca atctggtcct tctttcttat ctggtgaaat agtaacattt aatgtaaacg
     5761 tatctgttgg tttaaaaaca tcagttttag ctttagactc ttctacaact aatgaagttt
     5821 ctgtatcagc aggaataatt tctgccttat aatatgctct atgcccccaa taagcatcct
     5881 taaaaggata tatactcgaa aactgtgttg atgtaatgct taattgatat ttcccagcat
     5941 caacttgttt tgtaagatca tcacttccta aatttatatc tggttccatc gtttcctcat
     6001 ttaaacgttg agaactaata gtcataaatt ttttatctgt gagtttttca cctttaggag
     6061 tagttattgg aataccacca tcttttaata gccaatctaa aaatgatttt tctacgtctt
     6121 cttcaaatgg taatccatca tatcttttaa taatcaaata tggtggagta tcatcaatag
     6181 taaattcttc atattgcttt aaaaattgag aacctgaacc tatatcttta atataaaaat
     6241 catctggttt tagaacttct ccagtaattt tatctataac aactaaatta acagatactt
     6301 cagatccagt aaatttatca gagaaccata agtaaagaat cccttgagca tctgtataac
     6361 taggtgctcc ataaggataa atttttccat caattagcat tttaaaatca tcaactagtg
     6421 tagtactaac tttatcttgc cctaaatacg ttagagatat tttattcata aaaacttttg
     6481 ttttcttttc taaatcaccg tatgcaactg ctccacctaa acttttgaat gttgttgctt
     6541 ttaatgaacc acctgttact ataacatctc ccgttgtagc acttaggtca aaaatccaat
     6601 ctcccactcc tgtcatataa ggtaaaatcg ttcctccagt tacaagtata cgatatcctc
     6661 tatttgttcc tggattaaca catccatccc caaatgcact accatgtgca cctccatatg
     6721 aataggaaag tccaccatta attgtaatat tcccactata accagaacct gtgctaggat
     6781 ctactactga gggacttaat gcattctttg ttgttgtata gtatcctcca cctattccag
     6841 ctccatggta tgcaccttta gcagttatag taccaccatt gattgtgata tctccaccac
     6901 taccattacc gcctccacct attccagctc catatcctgt attacgactc gattcttgac
     6961 ctatagcagt tatagcacca ccattaataa taatatttcc tccactttca gtatttccac
     7021 cgccaatacc tgcagaataa taatttccaa caacttttaa acttcctcta ttattactat
     7081 ctagtagtgt aaggcgatct ccttttttta acttgtctcc attttctaga gttacatctc
     7141 taggaatttt tccaccctca gaaactataa actcgccttc aatatctcta tttacaacag
     7201 aatcatcaat aattagagta gtaccttttc ctgctctcat acctggataa tatacatcac
     7261 ctgactctag cttgttttca ctaccatctt gtaatataac atgtgcatat gcaccaccat
     7321 ctttatctgg tgttaaagta aatgggctat tagatgcagt tttaatattt actccatcaa
     7381 atattaattc agcttttaca tctttaggta taacaatcct atgtgttgtt ggagtatttg
     7441 gattattatt ttgaattgtc atttgttttc cactaagaat agttaaaacg ccacttgcaa
     7501 atttaaaatc gacatttatt tctccaccac taattttaaa gtctcctaca gatgtaggat
     7561 cttctgcagt ggcttcttcc acaactaatg cattcatatc tccttctgca tttcctgcat
     7621 tagaatcttc ttctgcattt cctgcattag aatcttcttc tgcatttcct gcattagcat
     7681 ctccttctgc atttcctgca ttagcatctt cttctgcatt tcctgcatta gcatcttctt
     7741 ctgcatttcc tgcattagca tctcctcctg catttcctgc attagcatct ccttctggat
     7801 ttcctacatt agtatctcct cctgcatttc ctgcattagc atccccttct gcatttcctg
     7861 cattagcatc ccctcctgca tttcctgcat tagtatctcc ttctgcattt cctgcattag
     7921 tactttcttc tatagtatct gcgaaagtat ttgacgcatt aaatgatgct actgatccaa
     7981 gtcccattga gatacttaaa atcattgata gtgctttctt tttacatttt tgttgcataa
     8041 attccccctc cccttaataa tttttaatat acactgtata catccttata tacactctaa
     8101 taattcttaa tatacactgt atacatcctt atatacactc taataatttt taatatatac
     8161 ttaaaactta tatttgtcaa tgaatataca gtttttttca accttatata ttataagtat
     8221 tcattatttg ctaaataact acaataaaaa ttttaattat ataaaattta tttaatcttt
     8281 tatataataa tacaaggggt aattaattga aaaaactagt aaaaatatta ataaatatta
     8341 tttgcattat tacgctaatt ttctctagcc tttcgattta cataaaatta tctgaatata
     8401 aaaaagctga tgaagtatat acggaactca gagaaaacac tatcaataat agcaaatatc
     8461 aagaacttta tgataaaaat aatgattata gattctggct taaaataaac aacgtaaata
     8521 tagactatcc tgtagtacaa ggatataaca atgatttcta cctaactcat gatttttata
     8581 aaaattatct tcctttaggt agtattttca tggattatag aaataatttt gaaaatgata
     8641 agagtttaat agtctatggt cattatatga aaaataaaac tatgtttggt cagctagaaa
     8701 actatactga tgaagtattt ttcaaagaaa ataatttagt agaaataaat tataaagtac
     8761 aaacttatac ttatgaaatt ttttcagtat atactgctga cttaattaac agagattatt
     8821 taagtataca ctttaataat aatgatgagt ttaaatattc tttgaattat ataacctaaa
     8881 aatcagtaca caaatctgat ataaaggtag atatttcaga taaaattata actttgtata
     8941 cttgtaaata tgaatttaaa gatgctctta ctatagttca tgctaaatta atttcaaaaa
     9001 agagacctat aagtaggttt gtgtaaatgc agacttatct acagtctttt ttgaacgtaa
     9061 aattggtaag tatagaataa tattcatatt aaacttttgt aggcaaagaa atgaaaaatc
     9121 aaagagaaat aattattaca gatttagatt accaagctga attaagaaag tgtaaaacta
     9181 tggaggatgt agttagtgaa gataatatat aatagtttaa cttataaatt atcttggtga
     9241 tattaatgaa gtttcaaagt atatattatt tattgatata aataaaatta aatctgttgc
     9301 taatagatgt acctttgttt ggagacaaac tattgaagaa atataactaa aatgttgaag
     9361 tagctacctg actttatttt gaaatgcgaa caagattttg gcattaagat aatttagaaa
     9421 gaatcactgg tacacaagga tgtgaactta gaatgaatag aagtattcag gcataaggag
     9481 ctttcaccca aataaaacat agtatgaact ttaaaagaat tctaatcaga ggcaatcaag
     9541 atattctttc agagtttata cctgtagtgt tagcctatag tatttctaag tttcacaaat
     9601 aagataatca ttaataaatg tagtatgtat attcatcctc tcaaaaatat tttttaatct
     9661 tacactttgt aaattattct attttaaaat ggtattttta tacctttatt tcttatgacc
     9721 gttttcaagc tcaaattttg cataaatata aattaatgaa aagcaagtaa aaaaagaact
     9781 gtcgcacagc aatttaaaat tactcattcg acagtcctta tttcgtggat ttaaatagtg
     9841 gagaagaatc acaagaataa tacccacgat taaaccttta tacaatacat tttatatgac
     9901 tttaccatca ctaatagtta ttatacaatc agccatctta gctatttcat cattatgagt
     9961 tatcataacc aacgtctgct tgtattcatt tacacaatac tttagtaaat tcatcacctc
    10021 attagttgtt ttactgtcta aatttccagt aggttcatct gcaaaaatta ttgatggctt
    10081 attggcaagt gatcttgcta ttgcaactct ttgttgttga ccacctgata attcattagg
    10141 aaatttatta atctgacttt ctaatcctaa cttagttatt aaatcattaa tataattatt
    10201 atctatcttt cctttagcaa ttaaaatagg aagaactata ttatcataaa cattgataac
    10261 aggtattaga ttatagtttt ggaatataaa cccaaattct ttacttctaa gattagatag
    10321 cttttcatca cttaaagtat ataaatctaa attatccata aatacattac ctaaagttgg
    10381 tttatcaagc cctgccatac agtgaagtaa tgtgcttttt ccagaaccac tttgtccaat
    10441 tattgcacta aatttatggg gttctatctc taaagtaatt ccatcaacag cctttatttc
    10501 attttctcct tttccatata tttttttaat atctataact tttaacatct tcatacattt
    10561 acctcccaaa ttattcattc tcttttatac cttcaataat attcatactt tcaaaatctc
    10621 tattacttac aactactgct attaaacata caaatacaga tatagcaaag aatagtaaag
    10681 attccttaat tgggaatgta aaagacataa aattctctat tccatagttt atatacgcat
    10741 cattattcca atttgtaaat ttaactagat ttataaatga tggaatcata gctattataa
    10801 agctaaaaac tgcatatgct aatgcttcat atataagcat ttttttcata tttttaagac
    10861 taacacctaa tgctctaagc gttgaaattt ctttttttct aattagtaga tttgttctta
    10921 tagtacagaa aatattaatt gatgctatta ttaaaactag tgcaattatt gctatttgtg
    10981 atttaatgta acttttatat atactttctg acatttcatg ataacttaat ctactattaa
    11041 atagagaccc tggtatactt cttgaaattt tctgtatctc attattaaca atctcatcat
    11101 atggattttc taaatttatg cctaattgag tgtatttttg ttctcctgtt atgtttttag
    11161 agtgaatatt acttgttata atttctagat ctggcataaa atcgccatct cccatagcaa
    11221 tccattcatc ctttaaaatt acagccactc ttaccttcat ctctttataa acaattgtat
    11281 tattttcttc aacaggaatt tttacagtta ttatgtctcc tatatttaat tctttgaata
    11341 taggctttat agtatgatca tcaactatat cataaaaata attatatact gctacatttg
    11401 gatatccatc agtgtccttt ttaatatcat ctaagctacc ttcttcaaca aagtcattta
    11461 acttttcatc tatataatca tcatcataaa gctttaaaac taatttatct tccattatct
    11521 cttttttttc tactccatta taggttgtaa attctttgtt tatatcctta ttattaaaag
    11581 taaacattcc atttgttatt tgaattttat gtatactttt aacaccctta attttttcta
    11641 tattctctac atcactatcc gtgtattttg atatttgcgg gtctgcattt actccaaatt
    11701 gcattgttat attatatcat tttgcatatc ttcaatttta ctgttgacct cttcttgcat
    11761 acttgagaat gtagtaatga atatatatcc tcctaaagca atagacaaca tagatatagc
    11821 agctctaacc ttatttcttc caatattttt aagtcccata aatccataaa atccaaatat
    11881 tttgcttact agtctgtcta ttccaagtct acttctgtga tttttaattt tgtctgtatt
    11941 tattattgat tcaataggtg atatcttgcc gattttaaaa gttggtataa tacaagatat
    12001 aaaaacagaa attattccaa ccaatatagc ttttataact atttcattat ttatatataa
    12061 tttagggtta agaccagctt cactgtataa aacttcgtta taattactaa ttcctatata
    12121 tgaatatgta accccagcta ctaacccaag tagtatacct attattgtta taattaatgc
    12181 ttgatagatt atcatacacc ttacgtttct tttctttgca cccatcagtc taaacagtcc
    12241 catttctttt actgattgat ttagtgtaat attgaaaata ttaaatatga aaattataga
    12301 tactgtagtt attaaaacat ttcttttaaa aacattcgga tcattcttat catcttctac
    12361 ataacgaagt tcccttacaa gaggtacatt tggtagaaaa cttttttgtc ctaacttata
    12421 ttttttcata agataatcac attcaccttc aatatttctc catccacttt taaaacttaa
    12481 tatactatta taactaatcg catcatgagg tataatgtta tttgtttcat cattgccata
    12541 agttactgct tcgtattgcc aaaccacatc atagtaccct tttggttttt ccataactcc
    12601 tactatttta aattctttat ttatagaaaa taatcgatta cttccgttag tatctttata
    12661 gttctttttt attgttaaat ttaatgtaga tcctactcct tcacttaaat tcattgcttc
    12721 tagtgctttt ttctctaata ctatttcatt gttgttttta ggtagtctac cttctattat
    12781 tttgttgtat ctacttttta gatactcttc gtttgatgaa gttaatataa atgagtttcc
    12841 ttttttatct gaaatacgtc ctaaattttg taccgaatta ccctttgata ctttttcatc
    12901 agtttctaat tttttagcat catcaagtgt caaatcacta aactctacat ggtatccact
    12961 gtaaagttca tgtattgcta ctacattgtt ttcattttgt gaatctttta taacactagg
    13021 tccaaatatc aacataacac ctaacataac tcctattatt aaaagtagtg ttctaagttt
    13081 atttttcttt aaatatgcta gtgacatttt taagtaaatc cccatataat cctcctattt
    13141 tactataaat tatttatcca actcatctgt taagtagatt ggtggatatt ttaaatcttt
    13201 taattcttca ttattttttg aagcttacta ccatcttcct catgataaac ttgcagtatc
    13261 accttattta catgtagtgc ataataatca ccactataaa aatattggta ttcataacct
    13321 ggataatttt tgtaaaatga tttataaagt acccatccta attctctttt gtatactttt
    13381 cttttgtaat acgctctata tcatcacctg aaactccttg atatgtcata tatttatcta
    13441 tatatttttc agcttcattc cattttctaa aatagttgtt gtatataata aatcctatta
    13501 tggctataat gcttataatt aaagttaata aaatagcttt gtattttctc atagtgaatc
    13561 ttactacttc aatttaaatt ctttaataag ttttaattct aaagacttag ttttatcatc
    13621 aaatttctta acttgaattt ttatatctcc caaattatca acctctatat ttccttcacc
    13681 tttatcaaat acttcttcaa tttttctaaa aaattctttc tcacttattt ctacatgaat
    13741 caatgatcta tatgttataa atagccatta tgaatttatc atcttctgac agtttttcat
    13801 tataagaaaa atctttaaca cattgaattg ataatatttc ctttgtcccc ttcttgtcct
    13861 tagtcattga ataatgtaaa acttggctag gttgttcttt tgtatactcc aagtttccca
    13921 aagacaaaac aaaacttcca tctgatttat tttcagctac cctaagacca ctattttcta
    13981 ttgattctga tattttcttt gacaagtctt cttgatacta gaaatcggtt caatagtata
    14041 tattttttca tttgtatctg aaaccttatt tgtgcatcct gttattaaac ctagcgttaa
    14101 gcttatagct ataaccatag ttattgtaat ttttttatct tcatataatc ccccttatat
    14161 attgaaaact cgttatttct cataaaaaaa agacgctatc tttttctatt aaaagataac
    14221 atccctatat gtttagtatc aatatatgtt ccccaactgg taggttaatc gacccaacta
    14281 tcaaaatttt gatttaatgg tatataaata ttcaatataa atttgtcttt tttatcttca
    14341 aatgataaat ctcctctata tttttttata gaagatttga tgctttaaat ccctattcca
    14401 tgtacaagtt tatctttttt atctgttaat aataaacttt tattctttct aattgaattt
    14461 attttactat tctcacattt aattacgaaa taggactttg agatagttcc ttttatttgt
    14521 atatatttat caatactttt atcatgtatc ttatcgcaag cttctattgc attatctaag
    14581 tatttgcaaa tatattagat acatctattg gttttataaa attcactttg gaaaaattga
    14641 tatcacaaat gaatttaatt tttctttttt cacttacatc acttttttca tttattatta
    14701 tatccaatat aacattacct gtattgtaaa ttgatttaaa ttctttaatt taatctttta
    14761 aactatttac atacccatca attttccttt tttatatttg agattttcta tacaatatat
    14821 atgattattt atatcatgat aaagcttctt aactttagcg tgtgattctt gaatacttag
    14881 ataatgagaa tactgcatat ctaacttatt ttttattact tcattttgca attttaactt
    14941 attttcctta attattttta taaacactat aataatgact aagctactag atagtattaa
    15001 cctaggtatc acaatatacc caactatata tatttcctaa tacccaccaa attgatacat
    15061 ttttctattt atataaatta ttaatatagt aaataaattt gttattaaag caacagataa
    15121 gtatatatag tttattttat tatctttatc tagtcccaat cttttattaa taaatttaaa
    15181 tatagcacaa gctactatat aattattatt ataagataat agtatatatc tactttagca
    15241 ttatcataac taaacactaa tagagcttcc tctgatgata ttacattact tgtaactaca
    15301 aaaaataaat ttatagatat aacatctttt attaaagggt gatatacgtc aaataagaaa
    15361 aatgaaacta tatatttaac aattcctatc ttataaaaat aattatagta agttcccaag
    15421 taataaatat caaataatag attatatcat atagattttt cttcatatta taatcatggg
    15481 tcttatcaac gttaaataat ataatgattt tagttataag ataagctaat aatacagtaa
    15541 aaaaagatat tttcttagtc ttatctgtat ctttatcgta agttaatata ctttttagta
    15601 tcattattgg tgtaaacaat gataatatta ataatatttt atttatatgg tttagcatat
    15661 tctatcacca ataaatttta gaaacctctc ttttatatct ttatatctat atctgctaat
    15721 aggtacttct tcttgactgt ctaatattgc agtatttgac tttatatttt ctacatagtt
    15781 taagttaatt atataacttt tatgacatct tacaagttcc tctatattta aatctttttc
    15841 tattttttct agtatacata tagcatcaaa agttttattt tttgtatgta ttagcatgtt
    15901 ctttttttga acttctatct atttttttgc tagagtagat tttgtatgtg ttagacttat
    15961 ttttattttt ttcaattttt ttgatacatg ttaatatgtg tttttctagt tcttgaaaat
    16021 ttattggctt taataagtat caataagctc ttacttcata tctttcctgt acataattta
    16081 ttagagatgt tataaatatt attatctact tttcttatgt ttcttgcgat atctatacta
    16141 tttagttctg gcatttttat atctaagagt agtgtatcct ctcaattaac tttgtaatgg
    16201 ttcttctttt gagctatatt tttttaattc gatattcttt gtttttattt tttaatatat
    16261 tatcaacttg attttttatt acgatttgta tttctttttc atcatcacaa gctgctattg
    16321 taatgatgac ctctcctagt attgtatttt tatttagatt cttcacttta ggttaacact
    16381 ttttaaatgt atttatcaat aatatttaaa ttaaaaatgt ataaaaatac ctatatccaa
    16441 ttggatatag gtattcgtgg atttcaatgg tggatacgag gggagtctat aagtggttat
    16501 ttgattgttt tgttggtttt tgtgaatgta ggtatatagc tatttagtta tgatattaga
    16561 ctgatttttt tggttatttt ttattgttta tttttgtggt tggggtatta ttggggtata
    16621 acttttaaag tttataaatt gagtcattaa taaatatcta ttgtttagtc aatctataat
    16681 ataataaatc ttaaatagtg taactattct aagtagctgc tatttttatt catacatatc
    16741 tttatataca tttatgagat tcaagtatgt acattcttat tctctaattt ttaaatatat
    16801 tatcttaaaa tggtaacagt tctacattta attaaattta tattttattg tacttatgta
    16861 aatataaact aatctattct ctaaatatat attgtgatat aattatatat aaattaaaat
    16921 aggagtatta tgtaaagatc ttcgctatac tcttatagaa aggatactta atatatggct
    16981 gttaattacc tagataacaa caatttagaa aatttatatg atgaaatgca ggccgtatat
    17041 cttaatgatg atagaccttg gataatcgga tatagttctg gaaaagatag ttcttgtgta
    17101 gttgaactta catttagaat gcttaaaaga ctacccaaag aaaaaagaca taaagagatt
    17161 tatgtaatct catctgatac acttatagaa aatccaataa tacttgatta tctaaaaaat
    17221 aatatagaca agataaatga aagtgctact gaacatgatt tacctataag tgcacagatt
    17281 gtatacccag aacttaatga tagtttttgg gccaatataa ttggtagagg ttaccctact
    17341 ccaaagtcta ttaaatatcg ttggtgtact gaaaggctta agataaaacc atcaaataaa
    17401 tttataaaag aaaaacttga acaaaaagat gttattgtac tgcttggcgt tcgtaaagaa
    17461 gaaagttctg ctagaaaatc gagaatcgaa aaaagagaga tagagggtta ccttcttacc
    17521 ccacatgaaa ccttaagagg taaaaataag ttagcttatg tgtatacccc tatagtcaat
    17581 tttagtacag cagatgtttg ggatgtactc tatcatcaaa atgataattt catagatttt
    17641 ggtggtgaat ttggaccatc tccatctacc tcatggggaa ctgacgctat ggaattattt
    17701 cagatgtata tgaaaggttc tggtgactca gatgaatgcc catttatagc agatgaagct
    17761 agtgctaaaa gtagctgtgg aaatagtagg tttggatgtt ggatttgtac tgttgtaaaa
    17821 gaagacaaat cattaaatgg atttattgaa tctggtcatg acgaattaaa gccacttttg
    17881 gaattcagaa gttggctaat ttctgagaga gataaaccta aaaatcgtaa aaaacataaa
    17941 agaaatggct ctgtcatcaa aaaagatggt agaatcatgt ttggaccatt tacatttgaa
    18001 gcaagacaaa ctattttaag aaaactttta gaaactcaac ttgaaatgca aaaattttat
    18061 cctgatttac aacttattca attaggagaa cttaaagcta tagatgatat ttgggataac
    18121 gaagaagact taactggtaa tatattatca aaaatatata aggaagttgt aggcaaaaac
    18181 ctaccatggt ctgaatataa aaaaccagta tttgatgacc ttactttaaa tattatatct
    18241 gaaaaatgta gtgagtatga tataaatatt gatttattta ataaattaat gatagataca
    18301 aataaataca aatattttag taataatact aaattaaaaa cttccataga aagaatatta
    18361 aatcaacagt ggcttcatca agatattatt gaaaagattg aagaagaaag caacaaggag
    18421 ttaaaatatg aaaataaata aacttgtatt gaaaaatttc agatcttatg aggaagaaac
    18481 caccttcaat ctaaatacta ctagtgaaaa aaatattata ttgataggtg gtaaaaatgg
    18541 tgctggtaaa tccacaatat ttgaggctat aaagctatgt atatatggac ctatagctta
    18601 taaatatcaa ggtttcaact cttcatatat atctcgagtt aaatctaata taaataataa
    18661 ttctctaaga aatagtaagg ttgacacctt agtatcagta gatatagaaa tcagtgaagg
    18721 tactgaaaaa aatacctata ccttaattag acaatggact tttaaagata aaaaattaaa
    18781 tgaaacattt tcagtttata aaaatttttc atttacacca ttaaatgctg atgaacttaa
    18841 ttattttgag aattatatta catctattat ttctcctaaa atatttgact ttttcttttt
    18901 tgatggagaa catttgtatg atttttttat aggtaaaaat tcaaatatac atctaaaaga
    18961 atctttatta tctctttgta attttgatac atttgatatt ttaaaaaaaa tgttaatttc
    19021 aactaacaga tccaataaga atgtttcaga tgagattaat attataaaag atgactattt
    19081 aaatttagaa gttgaaatca acaacttaaa ctctcaagaa aaaaatctat cttatgaatt
    19141 agaaaaaatt tcaagtgaaa ttgagaagtt acaacaagaa caattaaaaa taaagaaaga
    19201 attcaggaaa aaaggtggaa ttttagcaga agaaagagaa atgcttaatt ctagaattgc
    19261 tgaattagaa ggaaatagat ttataattaa tcaaaatata aaagattttt gtaatgacat
    19321 acttcccttt ttaattgtaa aaaaacaaat attaaactta gaaaaacaac ttaaagtaga
    19381 gaatgatagt atattatatt ctcatcttaa agataaatta aatattgagt atataaaaaa
    19441 tctactttca gataagatca tgtcttcctc attagatgaa atagctctaa ctgtttctga
    19501 ggctttaata gaagatataa aaccagatag ttatgaagat aactttgaat ctatccataa
    19561 gctttctaat gatgaaagta gtaaattatt atctttgata aataccattt taaattttga
    19621 taataattct atcattaatt cctttggaga acatcgaagt atttcaaatg aattggcaga
    19681 cataagacat aagttaaata ctagctttaa agatgaaact ttgaatacat atataaatga
    19741 tatatctagt ttatcagata aaattgattc tttattaaaa tcaaagaatt tgctagttac
    19801 ttctttagat aaattaagac ttgaaatagc tgaaactatc actaaaagag acaaatctaa
    19861 ggctaaatat atatatttac ttcaacataa taatattgtt gatatatctt ctaatcttat
    19921 acttatgtta gatgaaataa taacaacatt aacagaaact aaaattaaac tagttcaaga
    19981 taattttatg tatatattta aaaggattat tcaaaaagac aattttatta gttctataga
    20041 tattgatgcc aactttaata tttctttata tataaataaa atatataact ctttagaact
    20101 tgaaaattta gtagaaaata taggatctga agaaatggag aagaagttgg gaaaattatt
    20161 ttttgaggat ttatttaact tatatcgtgt aaaagataaa gatgatctat tcaatgctat
    20221 aaaaaataat actcaatcta catttttaaa tctaagaact aaagttgaca tagacgaatt
    20281 ctctagtggt gaaaaacaaa tatacattct ttgtttatat tgggctttac ttaaatcttc
    20341 agagatagaa ataccattta ttatagatac accttatgct agaatagatg atactcatag
    20401 aaataatatt actagtgaat atttttctac aataagtgat caagctataa tactatctac
    20461 taatactgaa atagatgaga aatcatataa gataataaag cctaagttaa gtaatgaata
    20521 cttgattgaa tatgataata aaaataaaaa aactatacaa aataaaggat actttttcga
    20581 ggtgtaatta tgggatttag acttaaaaca tccatacaaa caaaagaaat atttgaagaa
    20641 ttaggatcta gaatgaattt gaaaccattt gctctttgca aactagctat ctcaatctca
    20701 ctaagtaata gtgagccaat tgaaaactat gaaagcatcg ataataatgg tcttgaactt
    20761 aatagacaaa ctataaccaa tcaatatgat actctattta aaactttact tgaacagcat
    20821 gcaggtagac atttaagtga tgatgaatac tttcctaacc aagttaagct tcatatagat
    20881 agaggtgctc ttatgcttaa aaacagatat aattattcaa gcaacttaga acactttatc
    20941 actcaaatag tacaggaaaa taataatcta taaattaatt aatatttatt aaacataaat
    21001 aaattaaaac atgggataga caataaataa aattgcctat cccatgtttt aattataagt
    21061 attttttcta aattcattta ttttatttat taagttccca aaatgcttta tctctaatat
    21121 atataggtga gttctcacta ggcattttga ctaagctttc atgtttagct agattcttta
    21181 tcttattata tatgttatct tcttcacttt tattcttaga attgaatatg tatcttatgt
    21241 ctttattatt tatgtctgat acattatgta ttatccaagt tgaaatataa ttctgatact
    21301 cattttctga agttgaaaaa tgacttagaa gttgtgttga tagaatagtt cctactccaa
    21361 actctctacc ttccttcaat atatttttta tagatgtaaa gtttctactc aagaaattat
    21421 cagcttcgtc tactaatatc attttattta attgtctata ctctccattt ccagttatat
    21481 cacttgatcc aagtgccttc atttgactat agaataaatc aagagttatc gcaactacta
    21541 aattttgtat tcctgaatca taatatgata aatttataac tgtaactcca tttattaaat
    21601 caaataaatt tttagtcttc tcactatttg gctcaaatat ttcaaaatca ttaaaacttg
    21661 aaagagcagc atacaattta tcgtcttttt ttatttctcg atcatcaagg tatatcgata
    21721 atacatcttg cattgtaggg catgatttta cccatgtatt ttcatcatct ttttttattc
    21781 cttttttttc ataagctttc ataattaatt ctctcaaaat aacttcttgc ttagggccta
    21841 agccaaatgc ttttgctatt gtctctttta aagaattcgc tgtgtgaaca ggtagtagtg
    21901 gctttattct ttcaggtatt attaaagtta atggattata aggtaaatga tatatttcat
    21961 ataccttagc acttgtcgca tctacaaaat catcttttat gtagtcgcct ttataatcaa
    22021 aaatcagtat tcctattggt ttaccattta tgttatgttt ttgttctcta tgaatttgcg
    22081 ttactaaaga tttagtaaac tgtgttttcc cagttcccat tgttcctatt ataccagtat
    22141 taggatgagg cactttaatc gaatttgttg gatcccaaat aagttcttta ttattatttt
    22201 ttatatttcc aaatataatt ttcattaaac cacttcaatc ataatctttt gttttattta
    22261 gcataacatt agtatctaaa ataacattct tatccatatt ttgtatacct attgtattat
    22321 ctaaatactt atttataagc atttcttgta taacaaaatc actctctcct ctaatatact
    22381 tctccttcat ttcctctaaa tccatcacta tattattata tccttcttgt tctgaaagat
    22441 ttagtatagt tatattgcct tctttgccta aattcccatt ataataagtt ccctttttaa
    22501 atgatattac agctcctctt cctataaatt catctaaatc attacttatt tcataattat
    22561 cattcaacaa gtttgttctt atatcactat ttattatatc atcccaattt tgttcatccc
    22621 atatatgata taacttcatt ttttgtgcac ttactatagc gagttgtatc ataaaattcc
    22681 tgtacataag ctttgtaaac ttttgggaat cattttcgct ttcagtcaag tactcttcta
    22741 ttaaatctct tgtcttatta gcctgaatta ttgccttttt tatgacactt tcattattat
    22801 ctccaatttt cacttcaaca ggataaaaat atacttttat tttattattt ttattttcta
    22861 tcccaacaag taataaatca tctgaataag atccagtatc tcctgttagt ttcttaacag
    22921 taaacaaacc acctgcttgt tttaatccag ctccaccaga tactctaagt atttcttcaa
    22981 gagatattgg tatccatatt atatttggat gataaaaata agctaagctt atttttatag
    23041 ctgataatat gcttaatttt tctctaggaa actgagattt actagatata agccttaata
    23101 accaatctcc atttatagca ttgaaataat taatcaaatt agggattgtg tcttcttcca
    23161 ctttaatatc tttactattt agaaattctt caattataaa tttatattgt tcagattttc
    23221 tagtaactgt tatagcatca tatccactag atgttgtata ttgatcacta taatgtatta
    23281 ttaataagtc atttgaattc tcatcatcct taaaaaaatt taaatccact tttggatcta
    23341 taaaagtaac ccaatgagat gtcttataaa ttttatttaa aaatgaacta ttgttttcac
    23401 ttattgatgt tgtcacacta agattatcat taaatggatc tccagttcct attgattttg
    23461 taagtgaatt aaacttcatt gaaatattta acagttcatt tttagttgta gccataaact
    23521 tagatccaaa tccagtccta taagtatcct ctaaatatat tgatggaact cctgctatca
    23581 atccatttaa tgctacacct gtttttatat catccataga gctacttgtt atatttacat
    23641 ccttatccat ttgatataaa gtcaaatggg aatactcata taaatctgac attttatctt
    23701 ttttataaaa tttaacttta tctctaaata aatttaatac atcttcttca gagtaattgt
    23761 ctacacttaa atttacatta aaatgttttt ttatatcatt tattgaatta taaaatgcta
    23821 tttcttcaaa tgaatttata atacttttat ctccatatat ataaatatct attgatatta
    23881 agttatttat ttcctcacct ttacctaatt tctttatgta ataatcaaat ataccaagta
    23941 atacttcttt acaatcacct aaatttatta gctttatctt cattactgat ttacttatat
    24001 ccctaaataa atatgtaaaa tgttctataa attcatttat tttttccttt gtcaatttag
    24061 agacataatc tctagatccc ttatatctac tcatgtcatg atcaacatag tatgtccact
    24121 ctggtgaatg tctttgatct actaccttat ataaactatt cttaccttta tatatatagg
    24181 gcattagata cttcgaaccc aatagtttta agattccttc tttaacttct tcatcaccta
    24241 taacattatt tatcattaat tggtaagcca catttactgg atgtaatggt gttaaaaata
    24301 attcttcttc tttatctatt ctatgtatag tccctacctt agctatatta agtctctctt
    24361 tagataatac ttttccattt tttatatctt caaacacttc taatatagta tttatataat
    24421 tttttgatat gttaactaaa tccacatcat aatatgccaa gcttggaata caatcattta
    24481 ctttatagta acttataagg tttttgtatg cttcttttat tctggaatct acatccaatt
    24541 ctattatttt atatttttct tctaaaatag tacaatgcat tatattattt tctatgatat
    24601 atttttctat ttgcaaattt tctttaaaat tttcttttgg aaaatattca ctagtacctt
    24661 gtacaatttt actattacct aaatatttaa aactctcctt tcgttctctc tttaatttcc
    24721 aggttttcat tccacttata gaaattggtt gttctttttc ttcttgttta actatggtaa
    24781 ttacagattg atcatatact acatcaattt ttataaattc aacatcttct tccaattcaa
    24841 cattaagttc taaatctagc ctttctcctt cttcaagatt aatttcattt tcaaactcat
    24901 ctatttgtat ttttctctca attatacctt ttgggtttat tactattgta tcatcatctg
    24961 tgttagtaac taacatactt ctttttgaat taataacaat tccataatta ctttttatat
    25021 catttaatat attctcatca caagatacta tacaaattct aaattcatat tttattatac
    25081 catcattata tttaacttta gtaaatgtag tttctgaaat atttatattt atacttatat
    25141 aagctttatt tccacttgct tttacttctg tatatgtttt agacatctta tctatagaaa
    25201 aacttgtttt ttcaattttc ttatcaaatt ttatgcataa atttataata tcaagtttgt
    25261 ctttattaaa taataatatg ttttttcgtt ttgacttatt ttcaatatct ggtttactcc
    25321 agagctttag gttttcaaca cattctatac ataataactc tacctttttg tttttcattc
    25381 tttcttgaga tgactttata tctttatagg tagaatactt ccaattctca tcttttaaaa
    25441 gatctatacc ttttgaatca aaatgtttct ctaattccag cttagggtct ccatactgat
    25501 gtatagagtt tacaatagaa aacagctttt cattatctaa tagtctttct tttattgcct
    25561 tgctactttt gttgtctaac atattatcat cataaaataa tccaaattta ctatattcac
    25621 tcttttcaat tcttccttta tttaatatat ctaatatatt ttcatattga aatattgatg
    25681 aattatcttc taatatactt ttattcattc tatcaagtac aaaataaagt atatgtttaa
    25741 tatcttcttt taatttatca ttttctaatt tattttgtat gtcctttact aaagtttttg
    25801 tatgaaatgg cataccttct ttttgcaaac tttctgttcc tcttattaaa ctatctaatg
    25861 tcatattatg tataaataat atggctgaat ccttaaattc atcactagct acatttccta
    25921 taagatttct aagtctaatt aaaaaatcat tggttgtatt attatttgaa gcaactatta
    25981 attgaatacc actgacattc atactaaaag tctgatattt tgatccattc attatattat
    26041 atgtaaatgg tctactatta ctttctttct ctaaaacatc atacataatt ttaacttgtt
    26101 cttctctttc aaactgtata ttaaatttat cgccactttt tatttctcta gattcgaaat
    26161 atttaactag atttttagct agatatctat aaaattgatc tgacatattg tgcatcccca
    26221 ctatcactct ttttttctat tatatttaat ttttcaaata actctactac tttgctttga
    26281 gaatctctat caaaataaat tcctcttttt tcatattctt caaataattc ctttaatttt
    26341 atctttgtct tatttttcat acataatttt gtcataaata acagttcttc ttgattaatt
    26401 ttcaatacat ttcccaatgg ccctgctttt tttaaaaaat ttaatttaca atactcttca
    26461 aataaatttt tatacctctt atagttagcc tctcttgaag atttcttgaa ctgataatct
    26521 attcttttat gtaaatcaca tatttcttct tttatgttat cattcttata cactttagtt
    26581 tgttcatcat aatcatccca ttgtacatca gtaattgact tgacataagt attttttata
    26641 tctattatat tttcatataa cctaagtctt tcattttctg ataaattttt tataatttta
    26701 tttaaatcta cataatcata cttactaatg tcattattag tatttaatat ctctaatgta
    26761 tgaacatgac taaataatat tctaatatga ctctccaaca ttttccaacc catgttataa
    26821 cttattcttg ttttagatgc tttttcccaa cttaaaataa aatatacttt ttcagttcta
    26881 tctctatctc catcaaaaaa gtgagataat tttattgata attgtgatac atagaaaaaa
    26941 taatagtact ttaataatct ttcaaactcg tttataatta attctggata attaagcata
    27001 aattctaaat cttcttggaa taatttagac acatttttac atatacattt ataaattgat
    27061 ttagacgtct cttcattttc aagttcagga agatttttta atataataag ttccataata
    27121 ttatcaggtt catattcaaa acatttattt attttatttt ttatagttat attatctgta
    27181 ctatttagta aaacatcata taaaaattta gacaattttt tataataact atcagatggc
    27241 atatgtatat aatttaaaag ctttggatga aatatataca tattttcttc ttcatccaaa
    27301 aataattctc ttattatact ctcaagttgt acactatcat cagaagaatt cacatttatg
    27361 tcttttataa gtaactttat atcttttagc tgtctttctt ttatgttcat tttcttgtta
    27421 tataccaacc tacagaattc tccaacacaa gactcaaatc tttctacctt ttctttaagt
    27481 ttcgattttt ctttagttgt agttgtatat gggagtataa ctatgttttt gacactatta
    27541 tgtctcgcga tacctttatc tatcatataa ttttgtttaa agtattcttt atttattata
    27601 agttgcatac cttcacccca tttactctta taaatttgaa tttatcaaat tctttatcat
    27661 atgttaattt atatttaggc acagtatctt ctaatctatc ctctatatat agttctctgt
    27721 tttgatttcc taatttatgt atcttattca taaatactac aaaatttatg taagtatatt
    27781 tatcattatt gtttggtgta tatccatatt ttactctcat tagtaactta tataaattaa
    27841 aatctatatc cactgtacaa gtatcttgat tttctttttc atcattttta taccctatct
    27901 ctaaactagg cttaaacttg tgcaattctt cctcagtaca ttttcttaat ttgcttaaat
    27961 ctggaattat atttagcctt tgacttattt tatatctcat ctgatttttt ccaatggaca
    28021 tatttattct gtcatcttga atacgtccat tccaactata caaagcttct ttaatatcat
    28081 catataaatt acttaaataa gttttttctc ctttgttaaa atagtataaa tttctcatgt
    28141 aacttatata tatattatct cttaactgta aattattttt aggtataaat atatgtagtc
    28201 ttataaatgt atttattata ttgtatttta atattttcct actcagttcg ttctttgaag
    28261 aattcaaagc cgactctatt atttctctat ttgtcataga actttctaga taactgtttt
    28321 tacttaaatt caggtattga ttaaatagta ttgtcatatc ttttgtatta ctaagcttaa
    28381 tgattatatt atctaggttt tctgatgtga tatttatagg gtcaatttta tttactgcat
    28441 ttaaaatctg agataattca ctatgattat ataaattaga tacaaatata tattttatga
    28501 atgcttcata acttagttct tctacaaact taattaagtc tttattattt ttattgtcta
    28561 aatcaacatt aacaataaaa tcatatataa aattcaaaag agaccttgtt gaaattataa
    28621 ttttatattt aactgtagat tctatcaata tatccaccaa ggtttctctc actgagtctc
    28681 tcattaacat ttcataatta tttttaatcg gacatttata attgacaata caatgtgtac
    28741 aatttgtttt ataactattt ataaatatat tttgattatc ttctgctatt attttattaa
    28801 ttatagattc tatatatcta gatttaggtt tatctgcatt caaagtatat atatgataat
    28861 cactaaaatt aacaaaatga aaattcgaat ttttatcatc atcatctgat atagatatat
    28921 caagtattcc tttattttca acaaactctc ttaatttagt aaatttatta acatattctg
    28981 attcaagaaa attactaagt gcccctaaat ttattgctaa tactaacttt ttatctgaat
    29041 tatccatttt cttatctgaa aaatcatcaa gtacatcatt aagtgtatct attgatgttt
    29101 tatttggttc aaaactttct gtagcatcat tatgaattat aaattttgac ataatttctg
    29161 gatatttgtt atttaaatat gatattaaat gcgattttcc atctcctact ccaccacata
    29221 ccaatatcaa ctcagcttta ttagaatcag ctgtatattt tataatatca agtaattcgt
    29281 tttctacttg tcttttaaca tgcatatatt ttttaaaact actaaaatct gaaatatttt
    29341 ctactgcttc ctttgatgat tcttttaatt tctttaattc atctatcaaa caaccatttg
    29401 acataatttt aaaactcctt ctaatatact cttttatact acacttatat tataaatgct
    29461 atttatttct ttgagatccc tttatgtaaa aaaaaccatt attttatata atagtaaatt
    29521 ataattatat ataaagtata aatatatata attttgtttg ttatgtaatg gatatattta
    29581 ttctgaatac atctactcca actatacaaa acttatctaa tataattata attttgtata
    29641 gtataaagag ttgataacat ataaatatta tagtgactat gatgaattca aaaaaatctt
    29701 aaatttcaaa tataaaaata tattgtatat agtatactaa tataatgcta aattaaaaag
    29761 gacactttta atgtcctctt taatttagca ttatagaatt aacttataag cataagtaaa
    29821 ttaatccatt aattctatta taaattttgc tcctgtttta aatcctaatt tataactaag
    29881 ttccctttca aactctatta atgaattaaa caaatcattt aattcaacca acacatattt
    29941 cttatcatcc tccaaaactt tttctaataa ctcattaatt tttttaacct ctttccaact
    30001 ttcaatataa tatttgtctt tttctaaaat atctctatac atatgttcat ttatccaata
    30061 atcaaaatat atttctataa tttcatcata cattttagta cctccttgac tttcctcagg
    30121 cacttaatat atacttaagt tgctaggtta gtgtacatca agtacctaat ttataaaggt
    30181 agttgttata tttatatggc aactaccttt ttctaataag tgtttaaatc taaactaaat
    30241 atattttaaa tctttattac atctaattaa aatttatatt tatacttcaa cacttcttga
    30301 aacataattt atctattact taataaatca tgcttctttt ttatatctct tgtatcttct
    30361 tacattcttc aaaagaaaac aaatttccta tataccaata tggtctaaat cctgactcgc
    30421 catctctatt tttagcaaca ataatctcac ttaaattagc accaaaacct ttttctcttc
    30481 gtctcaactc tcttatatcc tcaattccca ttgtcttaca ataattaaga gcttcttttt
    30541 ctgtaggtct atgaatatat ataactgaat tagaatccat atatatctgc tttgagcttc
    30601 tcatcactct atcaccttta ggtctttcat ctttcaattc atcatttaat tgagcaagct
    30661 gaataactgg tatattaaaa tctaaagtaa tcttttttat ttgccttgat aagtcagcaa
    30721 atttagcttc tcctacttct ttaccttcat aatccaaaat ttgcaaataa tcaattatga
    30781 ctaagctagg cttaatactc tttatttctc tctttattcc tgatattgta tttacagagt
    30841 catttatata aagattattt tcctgattta ttatgcttaa agcttgtata tatttgacta
    30901 tttcttcatc atttagattt ctatttttca tctttacaac tgatattcca gttagtgatg
    30961 ctatatttct aaatagaatc tgtatatcgc tcatttctcc acttataaaa agtactttct
    31021 taccttgaaa tattacattt ctaagtattt gtaacgccaa tgctgtttta cctcttccac
    31081 ttttagctgc tattgtagtt agttcacccc catataaacc tcctatagta tcatctaaga
    31141 actttattcc agttttaata ccttcattta cttcatcatt tatatattca aaggctctct
    31201 ctgatatggc tgttatactt gaatcctttt ttattaattt ctgattagct ttatttatat
    31261 cattttctaa ctgatatata agagtattat catctatttc tccaagattg aactttttga
    31321 atttatcaat tatatttcta tttttgtatt tttcaataac tatacttaca taactcatga
    31381 aatttgatac tgttggaact attgttgaca aactagtgat atagcttata cctccaatat
    31441 tattcaaatg accttttctc tttaattctt ctgtaagagt tattatatct attacttcat
    31501 ttctcttatt tatttctttt atagcattaa atatgtattt atgttcctca tgataaaaca
    31561 tgtcttcatt taacatttgc tctaactctt gtactattgt tgaatctaat aaaatacttc
    31621 ctaatattga ttgctctgct tctgtatttt taattaagtt gtttgaatta tttataatat
    31681 aacctcctta ccactttaat tgtgagtcat ctggattata tattcctgtc tctttattta
    31741 tgacaatttc cttgctatta taatttccct ctagtatctt agtaaaatta cttgaattca
    31801 ttatccaatc aaaatttgct ttccatcctg tattattatt tccattcaga aaacttgttt
    31861 tactaactat atttataacc tctaatacag tttctttatc atattctttt attcttgaat
    31921 ttattctcct ctttcttgta tctgttagtt tcttgacctt tgaaagttca atacaagttg
    31981 agttaaattt gcttatgatt tcactatata taatattatt ttcattcttt atattcttga
    32041 ttgttgttga ttgattgtta atagtctgtt gattaggttg ttgattttct atgttctctt
    32101 gataaagctc ccagttttca atagttatga ctatatactt gtttgttgtt tcgtatgtta
    32161 gaaaaccagc atttttaaaa accaacaaac aacttctaac catttgataa ctaactcctt
    32221 taccacatct ttcagctatt ttgctatagg aagaaataaa ttgtccagat tttattttta
    32281 tccttttatt atctataaaa acatggtttt ctttatgact agccataagt aatagagtta
    32341 ttaatattac ttttctttct ggtttaatgt ctttccaaat tgatttatcc aacaactccc
    32401 tataaagctt tatccaccct ttattgctca attacaaaca cctactttaa ggctataact
    32461 tgataccatt tgcattttca ataaaccaat catctaattt attttttatt atgtatactc
    32521 gattatttat ccatatacaa gggaatcctt tgtttttaga atgacataac tgcctaaatt
    32581 ttgattctcc tataggataa agcttagatg cttctttaca tgttataagt tcagctttat
    32641 ttcttttcgc attttcaata attttatatc cttgcttttt ttcttgtgct tcttcttcat
    32701 tctttacaat aatgtttaaa gtggcttgta atgtctttaa aacctctaaa ttattattct
    32761 ctttcatatt ttaatcctcc ttactaaaaa ataattcttc tactgttgta tctaaagctc
    32821 tagatagttt aatcatcaaa tcattgcttg gattattagc cttacctgtt tccaagtttg
    32881 ataaataata tcttgatata ccaactctat ctgccagttc ttgttgcttt agaccttttt
    32941 taatcctaaa tatttttaaa ttgattccca tactttttcc tccttttttc tagtttacat
    33001 tcgtaaactt tacattttaa ttataatgtc gacatatgtg tactgtaaat aaaaatatat
    33061 tatttttctg ttctcatttg tgtacttttg ggctatactt tatacgaggt gataatattg
    33121 caaattggaa aaaacttaaa aaatataaga aaacaaaaag aactaacgca gattcaactt
    33181 gctgaaatta gtggcatctc aagaaatgct ctaataaatt atgaaaatga taagagaata
    33241 cctagtatag ataccttatc aaaactcgct aaagctttga aaattgaaaa gactatcttt
    33301 tattttgata ttgaaaatga agtaatgact aatgaagatg ttttatacct tttacagtct
    33361 ttattcccag aagaatacta ttcttttgat gaatttttaa atgctatttc aaatcttatg
    33421 gaagatgatg atattagaat tacctcaagt ttattgctag gtgcctggtc tgataaagac
    33481 caatattata aacttggtga aaaattagaa ctaaatgaaa atcaagttta taattggata
    33541 ctttcagatt gtatatctaa tactgaatta gatttttcat tacatcctga tgacataaaa
    33601 gaaattatta aaaatgatgt tcttacaaaa caatcaattg atgaattatt agatattggt
    33661 ctatcagaag aaaatataga aacaatctat gattacttta gtgactcaac agataatgga
    33721 agtaatattc gactaaaaaa attaatatct caaaaagaaa atattactat taaaaataat
    33781 gattacttta aattattaca agaaaataac gaactaaagg aaaaaaatga aaagcttgaa
    33841 aatacactta aagaaataaa aaaactacta actaaaatat aaaataatat caatactatc
    33901 taaagcaatg ataaaaaact atgaatggag ttacaattat gaataaatca tatatgctaa
    33961 aatacacata taaatgccct actaatttat ttcatggaga taaaaaatat actctaacat
    34021 acactaagag agttatgagt aatttagtat atgatatggg ataatctaga aaataactcc
    34081 tttacatgtt tagaagtaca atcattgttt gatgatatat ttaaggtgaa attatgatga
    34141 ataatacaat tggagaaaag attaaacaat atagaaaatc gataaatatg tcaagggatg
    34201 agctagccaa caagattggt tgttcagttc atgctatagc taaatatgag caaaatcaaa
    34261 gaatgcctag tttagatatg ataagaaaaa tagctactgc tttatcaatt gctataaata
    34321 aactcattgt tgaagaaaat gatttagata ctttaacaaa taataatatt aatttaaaag
    34381 tatttgataa agagttggat ttagtaattc acatattaga aaattatggt tatgatataa
    34441 aaacagacta taaaaaaaag tttgaaatta cagataatgc agatacaata aaaccaatta
    34501 acctaaactt ctctgacttc aaagacttct ccaaaaatct aaattgggtg ataaatggtc
    34561 ttgttgaaaa ttttatacaa gaaaactcta atggtcttat tgaagatgag gatgaagaat
    34621 attagtattg cacattttca aaatatgagt ttacctatta acttttatac attcatagtt
    34681 aataaataaa aattagatat gaaaggagct aaaatcatga ataaatcata tataccaaaa
    34741 tacacacata aattccctac taatctacct catggagata aaaaatatac tttaacatac
    34801 attagaagaa tgataagtga atttgtatat gatatgggta acctagaaaa taaccccttt
    34861 acattcccag aagtacaaac attacttgat ggtataaccg taggcggaca taaattaagt
    34921 gaccaaaatc aaatactaaa tataaaatca tcatgggatt atgtaataaa attaagtaac
    34981 aaagaaaact tatctttaaa taaagataca atttgctcta tacataaaat agttgcaaaa
    35041 gatgaagctc taatcgttgg agatttcaga aatggtaata taggtatatc tggtactact
    35101 caatatgaat gcatagaagc cactctatta aatgatttat ttgtaaatga cataaatatc
    35161 ataaataaaa taactaaccc cttagaaaag gctataataa taaatttatg gttatcctat
    35221 tgccaattct tctatgatgg caacaaaaga actgctagac ttacctcaaa cctaatactt
    35281 ataagtaatg atataggagt tctatcaata ccagctaaac ataaacaaga atacaacaca
    35341 ttaatgctaa acttttatga aacactagaa gctgatgaag ttataaaatt cttattagag
    35401 aaatgtatta cattcttcga tggatataac tataaatcat ataaggagtt gtttaaaaat
    35461 ggcaactaaa agagctaatg gagaaggctc tatcggaaaa tataaagatg gttggcgttc
    35521 tcgtataatg attggttata atgaagatgg aaaaccaata agaaaagaat tttatggtaa
    35581 aactcaaaag gaagttaaag ataaacttga tgcttataaa aaacaatatt atctaagttc
    35641 tgatatttca gatgataaaa taactttaga acattggttt tacaattggt tatttgaata
    35701 tagaataaaa gatttaaaac caaagtcatt tgaaagatat gaaagcatct atcgtaatta
    35761 tattaaagat actgatttag gcaatgttaa gttaaaggac ttaagagtat ctcacataca
    35821 aaaatattac aatagattgc ttgattccaa taaaagtata cctactataa ggcaaataaa
    35881 cactaaactg aaaacctgct tatcagaagc tgaaaaacaa ggtcttatac aacgtaatta
    35941 ttgtactatg gtaaatatac cagttgagaa aaaggaaaat aagcttgaaa tattgactct
    36001 tgaacaacaa aagagtttta taaaagctat agatggtcat aaattagagg tattattttt
    36061 acttgctctt ggtactggac ttagattagg agaattatta ggacttaaat ggtttgatat
    36121 tgattttaaa aagtctaatt taacagttaa aagaactctt caaagaacat attttataga
    36181 taaaactgga aatagagagc ttaaagtatt agagcaagag ccaaagacat caaattctta
    36241 cagaactgta cctattccaa aagatgtttt taataaattg aaagagcata aagtaaatca
    36301 aaattcggat atattaaagg ctggagaatt atatagaaat gataattatg tattttgcaa
    36361 tgaattaggt atccctatag atgataaaag acctttaagg aatctaaagt ctatattaaa
    36421 cagtttagat atagagccta taaagtttca tggtcttaga aaaacttatg ctactagact
    36481 ttttgagaat gatgttcctc ctaaaactgt acaagtttta atgggccatt atgatatatc
    36541 aataacttta aatatatata ctcaagttat ggaagataag aaggttgaag ctgtagaaaa
    36601 gttggataaa atattttcat tgtaaaaatc aaatacatat aaatattgta atttcatata
    36661 gttaattaca aaatcaaaat aaggatacaa attaatttta tattatcttg tatccttatt
    36721 tcgtatctag ttttaattat ttatcttgga tagcctttat gatacattaa tccattttct
    36781 aacattaatt cttgagccaa gtatatatga cccttacagt gcatatctgc cacaccttca
    36841 cttataagtt tatatgtttc agaagtatat aaataatcca tactttcttc caagctctta
    36901 cctgtttgct tagaaaattc taatatgatt tttttatata gtatttgtaa taatgtttta
    36961 tttgcttcca taatcactat acctcctctt catacctaaa ctttaaatat ttatctatta
    37021 cattttgatt ggttatacat atttgttcat ttggtttata gtattttaat ctttttaaag
    37081 cttcatcttt ttcaataaga ttatcctgat acagttctat tgtattgtaa atcttatcat
    37141 cagcaatacc acctattata atgtcatatt tcatatatat atcactcata tttctacatt
    37201 ctattataaa atcaagccat tctaaattat aactattgaa gatttttacc ttgcagtttt
    37261 cttttaattt atcaatatct aaagaataac tatttaaata tgctttttta cctctttttt
    37321 taaaacgctc tgtccagtta attgcttgtg tctttatact ggttacatag aatccttttc
    37381 caaaatctaa agaatctctt gaaaaggtaa tactaggagt ctttaccaca caatatgaac
    37441 catgatatac aatcattata tcacaccctt ttctttaagt acttgcatta aatcttccac
    37501 tatgtaacct ttactttgag aatgtagtgc ttcatagcaa ggtattatat agttatctaa
    37561 aatatcagta tcatgtttaa gaagtttata aactccttct gaatctatat tcaatttttc
    37621 tgctaaagct tcaatgcaaa aaatagaaaa ttctagtttt ctttcatcca ttacaaacct
    37681 caactcctat caagcttata taatatatat ttttactaat taacaactta aactatctta
    37741 catttataaa aatattatat cataaagtag atattaaaat tctatgttac ctattatcat
    37801 aataaacaaa taaaataata aacaaataaa aactttttat acaacctaaa tgcaccccaa
    37861 tataccccca aaaaaacctc ttttcctgtg gttggggtac ggttggtgga ttttatctcc
    37921 aaaataaaaa atactagtca acaaagacta gtatttccaa gcatttcatc attctttttt
    37981 taaaattggt ggagacgagg ggagtcgaac ccctgtccgc aaatcttttc cttgctgcat
    38041 ctacatgctt agccaacttc actaatatct cacctcacat agcgccaagt ggcaggctct
    38101 atgcttagcc agtcccaatt aatctacttc aagtcgagac acccctaaag tagttccctg
    38161 cataatgacg tccagtctta gtcggcagga ggactaagag ggacgagcag caactaggct
    38221 gctatcgcga aattatcgtc tgcgtttatt ttaaagttcc ccagttttta cgtgacccag
    38281 agtaacacga catgcagcct caagttccaa acccacgtcg aaacctttac gcccccttga
    38341 gtagtttata gtccagtaga cttttcgcaa actttatcac catttttatt aaatttttaa
    38401 tgtatatata atataatact tttaagaata aaaatcaatg ttatgttcta gataataaag
    38461 aattttattc taaaaaatac agttttatct agtttaatac ttacctgtca tttctctttc
    38521 tatacgtctt tgtgcatctt tcttagccat atcttgacgt ttatcatata gttttttacc
    38581 ttttgctaag gcaatctcta ctttaacttt accgtttttt agatacaaac taagtggtat
    38641 aagtgaatac ccctttatag tagttgctcc aataagtttt cttatttcat gcttatgaag
    38701 taataatttt ctaactctta aagaatcaac attgaatata tttccttgct catatggact
    38761 tatatgaact tgttttaaaa atacttcgct attatctaca gatgcaaatc catcacttag
    38821 gttaactttg ccttgtctta ttgattttac ttctgttcct tttaattcaa ttccacattc
    38881 ataagtttct tctataaaat attcatgtct tgctcttttg tttgttgcta atattttttt
    38941 accagccata taaatctcac cttctttgct agaaatttct tccataaatg ttagaaattt
    39001 actaaatatt taatgttgcc aaattatttg actaacttgt atgataacat atttaggcat
    39061 tttagtcaat atttcactga tatattaagt tagttaggct atatataatt aagtaatata
    39121 aatgtatgaa aatagctaaa aaactacttc gaatatactt aatactaaac taactctaac
    39181 cagtaatact aatttataaa tttcaaaaca aaaatattac atattata
//
LOCUS       DAGSRM010000031        38904 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.031, whole genome shotgun sequence.
ACCESSION   DAGSRM010000031 DAGSRM010000000
VERSION     DAGSRM010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38904
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.031"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(210..1229)
                     /locus_tag="MJE33_001727"
     CDS             complement(210..1229)
                     /locus_tag="MJE33_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBY9647663.1"
                     /translation="MMGKIRFGIVGTSNIANIFLRAASRVKDFELVAVYSRNLEKARE
                     FGTLYGASIFFDDLEQMAKSKDIDAVYIASPNALHSKQTITCLKHKKHVLCEKSLASN
                     LKEVKSMIKTAKDNNVLLMEAMRITCVPNFKAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPMVNLFGAPKTVKAVSHILQTGVDGEGSA
                     VFQYDDMDAIIQYSKVADSYIPSEIQGEEGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGMYYELVEFISLINNNKIESNINSHENSIIVMEIMDEIRRQSSIVY
                     PADSI"
     gene            complement(1405..1944)
                     /locus_tag="MJE33_001728"
     CDS             complement(1405..1944)
                     /locus_tag="MJE33_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HBY9647664.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKQGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFALLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSVYWTILTFIGVRYFQVYIVDSLYSLIIPYWPIVVTILLVTLF
                     VDEILTIKFLRGNKDIVER"
     gene            complement(2045..2725)
                     /locus_tag="MJE33_001729"
     CDS             complement(2045..2725)
                     /locus_tag="MJE33_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBY9647665.1"
                     /translation="MQNVEGIIFDMDGVLFDSERISLEFWMETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTDIYDSSVPIIDLYDEKTKNMIEFMERKGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDYFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PKNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILENLLEVREYLKSL
                     NSKECHNL"
     gene            complement(2754..4043)
                     /locus_tag="MJE33_001730"
     CDS             complement(2754..4043)
                     /locus_tag="MJE33_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBY9647666.1"
                     /translation="MRELLKVTLIVMLVLINAFFVACEFAMVKLRSSRIDTMITEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIVSVFNLSESIIYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLLVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRLLVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGAGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
     gene            complement(4507..6108)
                     /locus_tag="MJE33_001731"
     CDS             complement(4507..6108)
                     /locus_tag="MJE33_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBY9647667.1"
                     /translation="MIYDLILKNGFIIDGTGNPGFYGDIAIKDNLIAKIDSKINSNTN
                     KEIDCCGKVITPGFIDPHVHEEIVAILDGKFEKFLKQGVTTTINGNCGHSITPYSSEN
                     VYEYMYKNGLLLEEEKKYLIDKNKCWNNFTEYCDLISKSGISVNMGFLLGHGTIRWSV
                     MGGSKDRPPTEKEKNEITDIINDGMKSGAFGISTGLAYIPSKYADIDELVDIARQIKE
                     YDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDEILKARYNGVE
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELGIDGVMDALRNEEGRTLILKEAFIL
                     GDRGSIILLNTKDINMEKKSIREIATQKGIDEDKLLLDLLLDGDEELIFCLGGMYRAD
                     FPDKLHDDKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFPIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGILKEGLIADIAVIDMDNYSYPRSEDIDFSKPQLLANGVEY
                     VIVNGKIALENGEITENKCGEVLKR"
     gene            complement(6219..6896)
                     /locus_tag="MJE33_001732"
     CDS             complement(6219..6896)
                     /locus_tag="MJE33_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647668.1"
                     /translation="MSKVKSIYNEEYLPFMIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNISILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTILIARAM
                     YKDKLSD"
     gene            complement(6913..7608)
                     /locus_tag="MJE33_001733"
     CDS             complement(6913..7608)
                     /locus_tag="MJE33_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HBY9647669.1"
                     /translation="MDYLKLANHPLLWIASTVAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCMLEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            complement(7660..8859)
                     /locus_tag="MJE33_001734"
     CDS             complement(7660..8859)
                     /locus_tag="MJE33_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HBY9647670.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDIQE
                     AKVKSIVDEKIFISLHEHPVLFPEHLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDIYRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARNLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     AAKFDIKKITGTKEHPKVEYVKGLENPTETSKNIVRYLVKHGYSDEEIEKVLGGNILR
                     VLKQVWK"
     gene            complement(9120..11165)
                     /locus_tag="MJE33_001735"
     CDS             complement(9120..11165)
                     /locus_tag="MJE33_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY9647671.1"
                     /translation="MKKHNILFVSTDDKINIDISKQLENIFGEFCNIDNLVYVNRINI
                     ELSSYELVVCSDNDIKEYIHNNIDKNIPIVIVHRTINIENINQIISIENDSDVMVIDA
                     YKESADETAKIIRKLGLIHINLIPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGD
                     KVIDINTVIEIFTKLNISIDKLHIIKEKYNEDTVSGYRYYTTMNKTMKSFLEIIDEGI
                     ASVDKLGKFIYCNKVFSNLVGIDQNEIISNNFMDLFSDKVVKKIFFQEDEVNDEVVNL
                     NNKKLIINKVNVYENNERIKSIISIKNISAIQVLEDKIQNKLQSKGFVSKYTFESVVG
                     ESKIIKEKINIARKIATTDFSVLILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSL
                     SDTLIESELFGYDEGSFTGAIKGGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQ
                     EKEVMRMGGSKIIPIDVRIIAATNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERK
                     EDIPLISKYFLDEINSNKCFTEESMQALTLYEWPGNVRELKNLIYYIDTIVEEDRVDY
                     EHLPEQFRFEKNNTLVNENFDSIILDFKQSNFFEESICILTSIETWNNKNILLGRNKL
                     QEILKEKDIVLSVDQIRKRIDKLKSHGLLLSGVKKQGSFITDEGKNFISYVKIKGVI"
     gene            complement(11576..12385)
                     /locus_tag="MJE33_001736"
     CDS             complement(11576..12385)
                     /locus_tag="MJE33_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBY9647672.1"
                     /translation="MYYVYVNDINIAVYDLNPSAKKTVLFIHGWPLGHKIFEYQTNIL
                     PKLGYRTVSIDLRGFGKSDATSGGYTYSQLADDIYKVVHAIGLKDFTLVGFSMGGAIV
                     LRYMSLFNGYGVSKLVLAAAAAPSFVQRPPEFPYGMTREDVNKLIAQACTDRPEMVTD
                     FGEKVFASNPPESFRRWFNDIGFSASGIGTIGTAVSLRDEELFNDLKCVRVPTGIFHG
                     KLDEICPYEFAVFMNEKIEDSILYTFEYSGHAIFYDELKLFNQEFLQFLEE"
     gene            complement(12469..13002)
                     /locus_tag="MJE33_001737"
     CDS             complement(12469..13002)
                     /locus_tag="MJE33_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBY9647673.1"
                     /translation="MSDAHYDMHKRNGYSSDEPYPEIKVLGPNKYYAELLMDDYAGVS
                     SEFTSVNQYLYHNFDLDETHRELSEMWINISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGNSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            complement(13152..14501)
                     /locus_tag="MJE33_001738"
     CDS             complement(13152..14501)
                     /locus_tag="MJE33_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBY9647674.1"
                     /translation="MELKNFKDILFKKALSEGFEECEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     HYNDVKTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMLIGFVVPIIEDNGQKYDGIGYNIANSIEEIKPCEIAKLGVDNALSKV
                     GGKSIPSGKYKTILLNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTPFDDEGVATFKKEVVLNGKLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIEKGDKSLDEIIKDIDEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFDLLKDIEAIGEDLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            complement(14513..15895)
                     /locus_tag="MJE33_001739"
     CDS             complement(14513..15895)
                     /locus_tag="MJE33_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBY9647675.1"
                     /translation="MLSKNIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNKDKVSVMKVAYKSAKEFSNDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRIRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPTQKNILIENGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIAAGEDKPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFAVSEG
                     YLIKNGEIQEPVRGASLIGKGSDILMNIDMIGNNVKQAQGMCGSSSGSIPTNVGQPMI
                     RVKEITVGGR"
     gene            16139..17353
                     /locus_tag="MJE33_001740"
     CDS             16139..17353
                     /locus_tag="MJE33_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBY9647676.1"
                     /translation="MNQEYVKGNPIFTNINKVPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CTSALEELDTFIELYGNKCDYAKRDTLLYTANKLEVKAIKEEYNLRKENGFDVEYIDE
                     STNPFSFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVSFDDIENETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNNNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            17530..19443
                     /locus_tag="MJE33_001741"
     CDS             17530..19443
                     /locus_tag="MJE33_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY9647677.1"
                     /translation="MKYGGIIMKEILVIAPTKGTYEKSIHIVKKNKYTNIDVVFGNLK
                     EGIPLAEKSINHGTRIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSNEP
                     FGIIGFNNVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAH
                     IVKRLNCKGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVD
                     KNFKITLFNKSAEKIFGIKKENALYHNVVDVIPNTVLPQVVETMEPQIGEIQNIGSTK
                     IITNRVPIIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLSKGLSAKYTFDDIIHASEK
                     TKDCIELAKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSL
                     IESEFFGYEEGSFTGARKKGKAGVFELAHEGTIFLDEISEIPLELQGRLLRVLQEKQV
                     MRIGGDKVIPIDVKIICASNKDLKNMMRNGLFRQDLYFRINILTLYLPSLKERKEDII
                     KLSEFFIHKYSSKYNKTPLIITPNIKKALLEREYEGNIRELEGMMERAVIVESFDSIL
                     LEKLSNEYTNNIVDKDNSDFECSNLDLKTLEKKYIYKIYNENNRNTTKTCEILKITRS
                     TLWRKLKEIEDDV"
     gene            19714..20688
                     /locus_tag="MJE33_001742"
     CDS             19714..20688
                     /locus_tag="MJE33_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBY9647678.1"
                     /translation="MHILKSVKKIPGGLMVCPLLFGALFNTVCPQALEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGKPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIVALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVVLGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKSIFKTNKNIEVN"
     gene            20709..20984
                     /locus_tag="MJE33_001743"
     CDS             20709..20984
                     /locus_tag="MJE33_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBY9647679.1"
                     /translation="MNAIIHANEKDNLVTCVRPLIKGEKVSIDGKSYIVNDNIPVFHK
                     MATEDVKKGDVVYKYGEIIGIATVDIKTGDYVHVHNIESTRGRGDKK"
     gene            20997..22160
                     /locus_tag="MJE33_001744"
     CDS             20997..22160
                     /locus_tag="MJE33_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBY9647680.1"
                     /translation="MKLMGYLRENGQFGIRNHVLIIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIELTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKELASEIATTGKK
                     VDYVVIQECGGTLKTVSKGAEIVSKMAREVSKEVRVEFGMSEITLALECGGSDPTSGI
                     ASNPSIGVASNLLVDEGGSSILSETTEVIGAEHLLATRFEDEEMKDKFLKFVSDVEKR
                     AIAMGEDLRSGQPTPGNKAGGLSTIEEKSLGCMYKAGNKPFKGALEYADIVPPDKKGL
                     YFMDTPGQDIDSITGMVAGGAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEIFNEIIEVCNGKHTKAESLGHREFGIYRISSTF"
     gene            22497..24776
                     /locus_tag="MJE33_001745"
     CDS             22497..24776
                     /locus_tag="MJE33_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBY9647681.1"
                     /translation="MNFENTKNKKIIATLLLTLLLIVSLTCVTFIMNLNKVTHNETER
                     YLVEVSQSISTTINVKCKSIFRTLYSTGDTYFEINDSEKEVNKYLSKKLQLFEFDWLG
                     IIDSNGNFISALKDTGNVKGQDFYRKALQGKETITPVKSQSGTYGILYSVPLYKGDKI
                     VGAVAAWNTIDTMRNLLSVESFHGEGFSQIIDQDGNFIVSSANKNAPKNVVNFFDMIK
                     HQGKQVDKNKLEEMKINLKKNKTGSIYYTLNDGISKAMHYLPLQEENLYLLSVVPTKT
                     ASAQFDLLLKKAIFINILIVILFLLLIILIMFINQKNRAQLIKIAYVDSVTKGYSKAR
                     FDMEAKKIIQSSSSESYSLVSINIQKFKLINDSFGSEAGDKTLKYIHDVISNYLEPGE
                     LLCRVSNDQFNVLIKTIPQKEILKYIENITNDINSFNKNEECKYFIALSIGVYKIDDP
                     NLPLVSIQDRANVARKNNKGNIGNHPLYTCVFYSDLERLKMIKEKEMENRMEEALNNN
                     EFIVYLQPKISLKDNTIVGAEALVRWQDPNKGLIPPDEFIPFFEKNGFITKLDHYVFE
                     KVCSMIRKWIDNGETPIPVSVNFSRANLNNIDFLEKYRMLRNKYNIPAKLLEIELTET
                     LVFENLQKLINIIDQIHEEGFQCSLDDFGSGYSSLNMLKEIKVDTLKLDHAFFSSPNA
                     DNIAENHVIESIVNLAKKLNMNSISEGVETILQMDYLKKINCDMIQGYVFSKPLPQEI
                     FEKMTFGKKLEETSMDSSV"
     gene            complement(24871..26211)
                     /locus_tag="MJE33_001746"
     CDS             complement(24871..26211)
                     /locus_tag="MJE33_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBY9647682.1"
                     /translation="MENQEALRHEKIWILLFRYSIPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSIAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLVFLEEILFILGASKDSIFYAKDYMSVILVGSIFNLVAFSLNNAIR
                     AEGNPKLAARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVIHYFIR
                     GKSNLKLKKCNLKLNKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKQYERAKKTLILSIIVSVVILSFGFL
                     VVQVFPEVFVGIFNKDSDLMDIAVRGISLNLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLIIVLPIQFNLDGVWLAQPVADFIAMIIVLLFLKREFKTSRV"
     gene            complement(26223..27005)
                     /locus_tag="MJE33_001747"
     CDS             complement(26223..27005)
                     /locus_tag="MJE33_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9647683.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPNSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMEDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKKGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDMV"
     gene            complement(27020..28042)
                     /locus_tag="MJE33_001748"
     CDS             complement(27020..28042)
                     /locus_tag="MJE33_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBY9647684.1"
                     /translation="MKNIIKINSKTLLVISILMVLIFITFLLSLNMGSFSIEPMDVIK
                     TFLGQGQRNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALIGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSIWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            complement(28048..29061)
                     /locus_tag="MJE33_001749"
     CDS             complement(28048..29061)
                     /locus_tag="MJE33_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBY9647685.1"
                     /translation="MTKLSKKNITYLLILVSIVVLVFGIVLSITIGAKDMNLSTVIDS
                     LIKMEDGINMRIVKDVRLPRAIAAALVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFAISISLVLQKKLPQLIHGSFSVMMFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVKWEGIKLLFIAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTNFVRFICIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            complement(29189..30127)
                     /locus_tag="MJE33_001750"
     CDS             complement(29189..30127)
                     /locus_tag="MJE33_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBY9647686.1"
                     /translation="MKKIKSLAIFISIITTLVLVTACSDKNTEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPVATANVDSYETDKLPSYIREELKGVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSKL
                     FDKEEEAKSWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLARPKNMPKQDGITLPMVSMEGLTDIDADHIVVIATESDKKDLENSAIWAQIRAV
                     KDGNVTILDAAPFFSQSYNPIGKELLLESVKNELTK"
     gene            30397..30861
                     /locus_tag="MJE33_001751"
     CDS             30397..30861
                     /locus_tag="MJE33_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647687.1"
                     /translation="MVHLVYCDNAGKKGEKVLDKILAGTKTMVIRGAAGRKIPHSRVF
                     EDELLYFMEKGTAKISAKATVKKVENYVKLTEDEISKILNENQPKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIVEKIEDVVVGTSIPYNYEKSKF"
     gene            complement(30973..31290)
                     /locus_tag="MJE33_001752"
     CDS             complement(30973..31290)
                     /locus_tag="MJE33_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBY9647688.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ESK"
     gene            complement(31303..32034)
                     /gene="chbG"
                     /locus_tag="MJE33_001753"
     CDS             complement(31303..32034)
                     /gene="chbG"
                     /locus_tag="MJE33_001753"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HBY9647689.1"
                     /translation="MTKIIINADDFGYCEAVNYGIISAHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKTLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDCDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLVSLKPVIEKIVTKYNLPIRGGFEYNLEYSK
                     VVPLIDSFYKENVSEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLEKNGLVISSYRDI"
     gene            complement(32060..33370)
                     /locus_tag="MJE33_001754"
     CDS             complement(32060..33370)
                     /locus_tag="MJE33_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBY9647690.1"
                     /translation="MKKGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEAG
                     KEKLEIVGNLAKRMVKKAGVDMKINLTLDRREALRDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVESDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELREFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNKGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLAAKAA
                     VYGDYESALLALCINPLIPSDDLAKTILDEMLEAHKDYLPRFNR"
     gene            complement(33467..34780)
                     /locus_tag="MJE33_001755"
     CDS             complement(33467..34780)
                     /locus_tag="MJE33_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBY9647691.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEAYKNLMASIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQSGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            complement(34802..35113)
                     /locus_tag="MJE33_001756"
     CDS             complement(34802..35113)
                     /locus_tag="MJE33_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY9647692.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
     gene            complement(35313..36017)
                     /locus_tag="MJE33_001757"
     CDS             complement(35313..36017)
                     /locus_tag="MJE33_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY9647693.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVIANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADENLSSKLNISLEENVWEIKRVRLANDARVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCGYKIAMSEREVQAVISNEECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
     gene            complement(36108..37469)
                     /locus_tag="MJE33_001758"
     CDS             complement(36108..37469)
                     /locus_tag="MJE33_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBY9647694.1"
                     /translation="MKDRLNLTEGRITEKLLKLSLPIMGTSFIQMGYNMIDMIWVGKA
                     GSKAVAAVGTAGFFPWLAMAFIMISKVGGEIKVAQSIGANNISTTKSYMKSAIEINII
                     LAIIYTISLIVLNKQLIGFFRLGDLEVITMSRQYLIIVALGMVFYFINPVFTAIFNGL
                     GNSKTPFRINTVGLITNIILDPILIFGWGFAPKLGVAGAAIATVFAQIVVTTCFIYII
                     VKSKEEYFKLRLFKEIETKYYKVLYKLGLPIAIQSGMFTVFSMLLGVIVASWGPVAVA
                     VQKVGSQIEAISWMTAEGLSVALGSFVGQNYGAKKYSRINKGCKIAITVSSVLGIITT
                     LVLVFAGESIFSIFIDESEAIEKGAVYLKILGYSQFFMCLEIITTGAFKGLGRTYIPS
                     IIITILTGARVPLAYFISRPEMLGLNGVWWSITLSSILKGILMISLFISLLKLGKLYK
                     ISE"
     gene            37968..>38904
                     /locus_tag="MJE33_001759"
     CDS             37968..>38904
                     /locus_tag="MJE33_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBY9647695.1"
                     /translation="MKINKKFNRVDMQVSILTAIIVIISSSCVYFFNYTLTYKSMIYS
                     LSERSKHIYEYVERNIDKNTFTEINSKEDQSKKSYKSSKILLESVKNTTGVMYLYTAK
                     KTKDGSLVYVVDGLNSSRSDFRNAGDLIEKEIWGDLNKALDNHILLPKKIKSTSWGYI
                     FISYFPIHNDVGKVVGVIGIEFEAKHQYNTFKFISIVTPIIALLTCLISALIAVILFK
                     RISNPTYTDFANTDYLTGLKNRNAFEIDMNNLNNSNLKNLISIISIDLDGLKKINDKF
                     GHQTGDLYIKQASKIIQYVINKKYIVYRIGGDEYII"
BASE COUNT        13386 a         6601 c         4599 g        14318 t
ORIGIN      
        1 attgtaataa aatcgtcaaa actttttttg atactgttac aaaattgtaa aaaaaaagaa
       61 aaaaacgcca aaaataaatt tctttttagc actttttcat acttataatt tgacatttta
      121 acacttggtt aagtatacac cttaccttaa cttaaaatta aatttattta agaatatata
      181 ttataaattc ttatattttc taatataaac tatatactat cagctggata aacaatacta
      241 ctttgtcttc ttatctcatc cataatttcc attactatta ttgaattttc atgagaattt
      301 atatttgatt ctattttatt gttatttata agacttataa actctacaag ttcataatac
      361 attccttcta tttctctagg cttttcctct gctttttctt ttggaataga tatatcttct
      421 tctcttccat ctctgtattt aataatagct ttttcaaata gattaagctt ttcaattatt
      481 atacttccct cttctccttg tatttcagat ggtatataag aatcagctac tttagaatac
      541 tgaattatcg catccatatc atcatattga aatacagctg aaccttctcc atccactcca
      601 gtttgaagta tatgagaaac tgcctttaca gtttttggag caccaaataa attcaccata
      661 ggatgtatac aatacactcc tatatccatt aaagctccat ttgacaactc ttttttaaag
      721 gcattttcta taattccatt tttatattta tcatatcttg aagaatactg acaatagctt
      781 ccaaaaaatc ttcttatttt ccctatttta tataagtttt ctttaactgc tttaaagttt
      841 ggaacacatg taattctcat agcttccata agcaatacat tattatcttt ggctgttttt
      901 atcattgatt tgacttcttt taaattagat gctaacgact tttcacaaag cacatgtttt
      961 ttgtgtttta aacaagtaat ggtttgcttt gaatgtaatg catttggaga agctatgtat
     1021 acagcatcta tatccttcga tttagccatt tgttctaaat catcaaaaaa aatacttgct
     1081 ccatatagag ttccaaattc tctagctttt tctaaatttc tagaatatac agcaaccaat
     1141 tcaaagtctt taaccctaga tgcagctctt agaaatatat ttgctatatt gcttgtccca
     1201 acaataccaa atcttatttt acccatcata aatcactccc cattttctac actttatttt
     1261 aattttaata aaaaacttta caacttatag atatattata aaataatttt atcaaataaa
     1321 aaagaaagct aattttaaat gatttcacca ttttaacata gctttctttt aactacaatc
     1381 taaattcaac ttatattttt aaagttatct ttctactata tctttatttc ccctcaaaaa
     1441 ttttatagta agaatttcat ccacaaacaa tgttactaaa agtatagtta caacaattgg
     1501 ccaataaggt attataagac tatataaact atcaactata tacacttgaa aatatctaac
     1561 tccaataaat gtcaatatag tccaatatac agaaaacttt agacatacta ttcctctact
     1621 attgtactta taatctgaat aatcccaata gaccttatta aaatatactt tcataagtat
     1681 tcctgtaata tattctacta ctgtcggcac tactagacat aataaaagca atgcaaatat
     1741 attattgggg tcaataaatc ttgagacctc tattataatc gacattgcaa ttgcatacat
     1801 tggtttaaat ggaccattta aaaatccatc ttcttgaaaa tgcccttgtt tataataact
     1861 atataagttt tcaattatcc aacctataaa accatacaag aaaaaattaa ataaaaaata
     1921 taatagagaa cctacattat ccatgaatat tcctcctagc tgtttatact tattttttct
     1981 catattcatg gatttattct aaatcaaaga aattatattt aatttattta attaatactt
     2041 tatattatag attgtgacat tctttagagt tcaaactttt taggtattct ctaacttcca
     2101 gtaaattttc aagtatctta tgtgattgtg atttaatttg ctcatctggc tcttttaaat
     2161 ctggtacatt tatacatctt ataccaccat tgtatgcagc ttcaactccc ataggcgaat
     2221 cctctatgac aatacaattt ttgggattaa catttatctt tttagctgct tttaagaata
     2281 tctcagggtt tggtttagag tttacaacat catctccaca aactatagca tcaaagtaat
     2341 cttttaaatt tgccttagct aatctcttta ctgctctttc tctctttgtt gatgttgcaa
     2401 cagccatttt gtatccattt tcctttaaaa aagagattag ttcattgact cctaatttga
     2461 taggagctcc cttcctctcc ataaattcaa tcatattttt agttttttcg tcatataaat
     2521 ctattattgg tacagaacta tcgtatatat ctgtaagacc ttctattatt cctttacgat
     2581 ttcttcccat aactgatgta taaatttcct tagtcatagt atatccatac ttttcaaacg
     2641 tctccatcca aaattcaaga gaaattctct ctgagtcaaa caaaactcca tccatgtcaa
     2701 aaataattcc ttctacattt tgcatacaat tcctccaata ttttttgata taattatatt
     2761 acacttttta tgattaattt tacaaccctc aatttatcca attctaatat tgtaaaattg
     2821 taattttcaa aagttacact ttggtttact ttaatatttg aaccaagcat ataggatatc
     2881 catccaccta tactgtcaaa tccatcatgc tctatatcaa gtccaaaata cttattaata
     2941 tagttgatag attcagttcc atcaactaag taagaacctg ctcctatttt cttaatatga
     3001 gtctcttctt tgtcaaattc atcttgaatt tctccaacta tctcttctag tatatcttct
     3061 attgttacca caccagatgt accaccatat tcatccacaa ctatagcaag ttgaagtttt
     3121 tcctttctta ttctctcaag agctttatct atagttaaat tttcagatat atatataata
     3181 tctctcaata tttcttctag ttcaatttta ttttcattta tcttttgatt gtataaatct
     3241 cttatatgta caaaacctaa tatatcatct ttattttttc tgcatactgg atatcttgta
     3301 acaccttcct cttttaaaat agctaatatc ttttcttcag aatcactttc atatatacat
     3361 accatatcag ttctaggaac cattatctct ctaattttct tttcttcaaa ttcaaaaata
     3421 ttatcaacta atctttgctc tgattcatct ataagtccac ttttataact ttcttctact
     3481 aatagtctta tttcgtcacc tgtatggggt tcatcaacct catcagcttg tgaatatcca
     3541 aatggtttaa gaaataaatc agtacttaag ttgaaaaaat atattattgg atatgttaac
     3601 ttataaaatc ctactaataa aaaagatgta ctcaacatta ttttttctgt attataaagt
     3661 gctaaagctt taggaaccaa ctctcctatt acaacctcca tcattgttat aattaaaaaa
     3721 gaaatggaaa ttgatatagt atatataata ctttcactaa gattaaatac acttactatt
     3781 ggtaaaatta actctttaac tgtagattct cccatccaac ctaatgcaag tgaacaaagt
     3841 gttataccaa gttgacatgc agaaagatag gaatttaagt tgtcttttat tattttacat
     3901 ctttttgcat tattattacc ttcagttatc atggtatcaa tcctagaact acgaagtttt
     3961 accatagcaa attcacaagc tacaaaaaaa gcatttatca aaacaagcat tactataagt
     4021 gttactttta ataactcccg cataaagtat tggttcttat aatgtaagaa atctataaga
     4081 actcaaagtt ccccccttat attaataaat ttaatttata atatatattc tatcatattt
     4141 attaatatat cctactttat tacaaataat ttttataaac taaataaccc cttttaatat
     4201 ttatgactgg ctatttgata aatattaaag gggttattta gttatcattt tttatttcta
     4261 aaaatctttt ctcaaatgat ttagttatct atttatccaa ttttttacat aatttaatga
     4321 ttaaaaatat taattttttc actataaagt ataaatattt tttgtataaa tattatctga
     4381 gtattaaaat aaatattaat actataaata aaaacgaagc ataattttta tttaaataaa
     4441 tctcaaactt cgtctttact tacttagata tttatttact ttctaaatcc tattattttt
     4501 agaaccttat cttttcaata cttcaccaca tttattctca gtaatctctc cattttctaa
     4561 tgcaattttt ccatttacaa ttacatattc aacaccattg gctagtaatt gtggctttga
     4621 aaaatcaata tcttctgacc taggataact atagttatcc atgtcaataa ctgctatatc
     4681 agctatcaac ccttctttaa gaatacctct attttttatt ttaaattgcc tagctggtaa
     4741 tgaagtaact cttcttacaa tctcttccaa tctaacacca gactttctgt acatattgaa
     4801 gaaaattgga aaagctcctc ttctttgtag ttcaaaccat gacatatccc cacccacact
     4861 aaacaaacaa tctgaaccaa ccataacaaa tggattatca attattttat catcatgtag
     4921 tttatctggg aaatcagctc tatacattcc tcctaaacaa aagataagtt cttcgtctcc
     4981 atctaaaagt aaatcgagta ataacttgtc ttcatctatt cctttttgtg tagctatttc
     5041 acgtattgat ttcttttcca tattaatatc tttagtgttt agaagtatta tactccccct
     5101 atctccaagt ataaatgctt cttttaatat aagtgttctt ccctcttcat ttctaagagc
     5161 atccattacc ccatctatac ctaattcaaa tagactagat gatattgcca taataaattg
     5221 aattactctt tttttcttca tacaatgccc agaacttctt ggtactgtgt caaccattat
     5281 ttcaacacca ttatacctag cctttaatat ctcatcaaat gcttctattt caactggact
     5341 taagtgagat acttgaaccc tagcaccact tttttgccca acctctatgg cttcttttac
     5401 tgcattatat cttcctatgt aatctctaat atgagatgta tatataccat catactcttt
     5461 aatctgcctt gcaatatcaa caagttcatc aatatcagca tacttacttg gtatatatgc
     5521 caatccagta gatattccaa aagcaccact tttcattcca tcatttatta tatctgtaat
     5581 ttcatttttt tccttttctg taggaggtct atccttagaa cctcccataa cactccatct
     5641 tatagtccca tgccccaaca agaatcccat attgacagaa ataccacttt tagatataag
     5701 gtcacaatat tctgtaaaat tattccaaca tttattttta tctataagat actttttttc
     5761 ttcttcaagt aatagaccat ttttatacat atattcatac acattttcag aagaatatgg
     5821 tgttatagaa tgtccacaat taccatttat agttgttgta actccttgtt ttagaaactt
     5881 ttcaaattta ccatctagta ttgctactat ttcttcatga acatgagggt caataaatcc
     5941 aggtgttata acttttccac aacaatcaat ctctttattt gtattgctat ttatcttaga
     6001 atctattttt gctattaagt tgtctttaat tgctatatct ccataaaaac caggattacc
     6061 tgtaccatct attataaatc cattttttaa gattaaatca tatatcaaaa tattccctcc
     6121 ttaaaaacca atttttaaat aatcttaatg taatttttat atttgaaaat caaattatta
     6181 aatttcatat ttgaaaattc actattattt cattgtaatt agtcactaag cttatcttta
     6241 tacatagctc ttgcaattaa tatagtacca aatacacata taagaactac tagagactgt
     6301 ggtattatac cttgctttga taatataata gtcaatacag atattacaat tgctactaga
     6361 cctaattttt tatcttgtaa aaatacttgt ccaaaaactg acccaaatag agctggtaaa
     6421 attaagttta atttttctac tacttcagct ggaattttag atagtacgct tcctcctaaa
     6481 attaccccta tagttagtat acttatattc actaaaatag atacagctat tcctattgta
     6541 gatattatcg agccttcttc tgtacctgat tcaacttctg ctgccttttg agctgctatt
     6601 gaacatggta gcctcatatt agatatatta ccagaaagaa aactcatata agttcctggt
     6661 atacctaaga ttggtgaata agaaactggt tctattatgt aatttggagc actcatactg
     6721 acaattgcca ttgtacttgc taataatgct gatattggag gcataatacc aaatccaaag
     6781 ctcaaaatta ttccaggaac taatgctgct ataataccca aagatagtgt taatcttcca
     6841 taccttatca taaaaggtaa atattcttca ttgtatatag attttacttt actcataaaa
     6901 ctaactcccc ccttatatta ctgaagctat tatcattccg ctaaacattg atattgtaag
     6961 cccccactct cttaaccatt ttatattttt cttcttttca agcatacaca atattaccat
     7021 tatagccatt ccacttatac atgcaattgt tccattatca aaacgcaaca ccctatcagc
     7081 atttagatat gcaaatgaac ccaacattgc tcctagagat attattggta taaatgattt
     7141 cttgccacta gatataacat tggttatctt atctagttta tgtccaaata gtaatgtgaa
     7201 tattatccat cctaaagaac ctaatatcat agtccaaact gcatttgtaa atgcaagttt
     7261 tgtcatttca ggagaaccta aagttatacc taatgcatct gttccaaatc cggctgacat
     7321 tgattcaaaa attacagaac ctataaatga taatctcatc catgctatag gcccacccat
     7381 agttactata agcgatacca ttccagcaaa tatagtcatt gaaggcccaa tagctgaaat
     7441 agcactactc ttaacagctg actttatttg tttatctgtt attcccattg ttttaccaac
     7501 tttgcaagat tttatagcaa atataaggga ttgtaccact actattgata ctgctactgt
     7561 actcgctatc cacaaaagtg gatgattagc taattttaaa taatccattt ataaaacctc
     7621 cctataaatc ctcaaatttt attaatattt ctaatatatc tatttccaaa cctgttttag
     7681 tactctaagt atatttccac ctagtacctt ctctatttcc tcatcactat atccatgctt
     7741 tactaaatat ctaacaatgt tttttgaagt ttctgttggg ttctcaagac cttttacata
     7801 ttctactttg ggatgttctt ttgttcctgt tatttttttt atatcaaatt tagcagctag
     7861 tactgagtgt aattttacat ggtcgccata tactgtatct ggtccaaaag ctacatggtc
     7921 tattcctact aaatttttaa tatactcaaa atgttccata aatgactcta ttgaatgttc
     7981 tttattcttc tctgttattg tagtatgagg tgctgcttct acacctataa cccctccttt
     8041 tttagcacat gctattaata cttcatctgg agttagtctc tttgtgttcc ataaatttct
     8101 tgccccaaca tggcttaata ttattgggtc cttgctatat tctattacat caagtgtagt
     8161 ttgaactcca acgtgagagc aatctattgc cattccaatt ttattcattc tttcaacagc
     8221 ttttttccca aaatttgtaa gacctccatc tttctcttct tttagtcctg aacctaatgc
     8281 attggattcg ctgtatgtta tacccattaa ccttatacca aatccataca gtatatctat
     8341 tctatcaagt tcattttcta ttggtgctgc accttctaat gttggtatca atgctacttt
     8401 accttcactt ttagctctgt atatatcttc tactttttca cactttatga caaaatcttg
     8461 atgagctaaa tcacataatc tcatgcctaa atctattaat atatcatccc atttccaccc
     8521 tgaatttgat gttatcgtac atacaccatc cattaaatta tcaaatatag catcataata
     8581 tcctcttgat aatgcttcat aagcacatct ttgtttacct tccctattat attcaaatat
     8641 atctctttct aggtgttctg gaaataatac tggatgttca tgtaatgata taaatatttt
     8701 ttcatccact atacttttaa ccttagcttc ttgaatatca tctaattcaa taaggtattc
     8761 tttaactctt ttatctcctt tacacatctc aatagatgaa atatcttttc ctttttctaa
     8821 ataactatag gcttcatatc ctttgtaagc tgacttcata acaacatcct ccaagtttta
     8881 caatttttta tatttttcct atgtagcaat tgatgtgcca aaatataaat tttaattgta
     8941 taataaatct tgcaaaaaat aaaaacagta gtaaggttga tatttactac tgttttatta
     9001 aaaataatct tcttagatta tttattcaat atttttgaat tatcttaatc ttaaagataa
     9061 ttttatttat ttctatccat tatagggatt tttttagggt gttttgcctt tttagtcccc
     9121 tatatcactc ccttaatttt tacatatgat ataaagttct ttccttcatc agttataaaa
     9181 ctaccttgtt tttttactcc agataaaagt aaaccatggc tctttaactt atctattctt
     9241 tttctaattt ggtctacact taaaactata tctttttctt ttaaaatctc ttgcagttta
     9301 tttcttccta ataatatatt tttattattc caagtttcta tactggtcaa tatacatata
     9361 ctttcctcaa agaaattaga ttgtttgaaa tctaagataa ttgaatcgaa attttcatta
     9421 actaaagtat tgtttttttc aaatctaaat tgttctggca aatgctcgta atctactcta
     9481 tcttcctcaa ctatagtatc aatataataa attaaatttt taagctctct tacatttcct
     9541 ggccattcat acaatgttaa agcttgcatt gattcctctg taaaacattt gttgctattt
     9601 atttcatcta aaaaatactt acttataaga ggtatatctt ccttacgttc tctaagtcta
     9661 ggtatctcta tatgtaatac attaatccta taatacaaat cttctctaaa ggaaccttct
     9721 ttaatttttt tctttaaatc tttatttgtt gcagctataa ttctaacatc tattggtata
     9781 attttacttc cgcccattct cataacttct ttttcctgta atactcttag caatctctgc
     9841 tgtacatcta aagatatatc acctatctca tctaaaaaaa ttgttcctgt gtgtgctctt
     9901 tcaaatatac ccattttccc accttttatt gctcctgtaa aacttccttc atcatagcca
     9961 aaaagttcac tttcaataag tgtatctgac aaagaagata gattaactgc aacaaatggc
    10021 ttgttttttc ttagagattc attatgtatt gcctgggcaa atatttcttt tcctgttcca
    10081 ttttctccaa gtatcaacac agaaaagtct gtagttgcta tttttcttgc aatattaatt
    10141 ttttctttta ttattttact ctcacctaca acactctcaa atgtatactt tgatacaaaa
    10201 ccttttgact gaagtttatt ttgaatcttg tcctcaagca cttgaattgc acttatattt
    10261 ttaatgctta taatactctt tattcgttca ttattttcat aaacatttac tttattaatt
    10321 atcagttttt tgttatttaa atttactact tcatcattaa cttcatcttc ttgaaaaaat
    10381 atttttttga caaccttatc actaaataag tccataaagt tattagatat aatttcattt
    10441 tggtctattc caactagatt agaaaatact ttgttgcaat atataaattt tcctaattta
    10501 tcaacagaag caataccttc atctataatc tccaaaaaac tcttcatggt tttattcata
    10561 gttgtataat acctatatcc acttactgta tcttcattgt atttttcctt aataatatgt
    10621 aatttatcta tagatatatt tagttttgta aagatttcta taactgtatt tatatcaatt
    10681 accttatcac ctatgtcaat tatttgcttt atgttttgtg gtatgctgtt tctgcttcct
    10741 gtaataattc caatctcaca tttagactta tcacaacctg gataatatgg tattagatta
    10801 atatgaatta atcctagttt tctaattatc ttggcagttt catctgctga ctctttataa
    10861 gcatctataa ccataacatc agaatcattt tctatactta ttatctgatt tatattttca
    10921 atatttatag tacgatgtac tattacaata ggtatatttt tatcaatatt attatgtata
    10981 tattccttta tatcattgtc tgaacatact acaagctcat agcttgacag ttcaatattt
    11041 atcctattta catataccaa gttatctata ttacaaaatt ctccaaatat attttccaat
    11101 tgttttgata tatctatatt tattttgtca tcagttgata caaataaaat attatgtttt
    11161 ttcatagtcc cacctcctgt ttaacgagta tagaaagtaa taatttggtc tattgtagca
    11221 ctttttaaaa aagttttaaa tcttaaaaat caaattttgt accatttact attattttcc
    11281 ataataaaat aatgtaagct atcttttttc acatataatt taatttctaa aacggttagt
    11341 ttgacattat tggaatataa acaaaaattt aatataaatt cgttaatata ttaatacatt
    11401 tttaagaatg acaaggaata aattataaca aattttagga atatataata atagggtctc
    11461 agaacttagt tacaagttct aaaaccctat ttgtttagtg taaaaaacat cataaaattt
    11521 agcttaaatc ttttcacata atattactct ttttctaagt gataataaaa tatatctatt
    11581 cctctaaaaa ctgtaaaaat tcctgattaa atagtttaag ctcatcatag aatatagcat
    11641 gaccactata ctcaaaagta tataaaattg aatcttctat tttttcattc ataaaaactg
    11701 caaattcata cggacatatt tcatctaatt tcccatgaaa tataccagta ggcactctta
    11761 cacattttaa atcattaaat aattcttcat ctcttaaaga tacagcagtt ccaatagtgc
    11821 caatacctga tgcactaaaa ccaatatcat taaaccatct tctaaagctt tctggtggat
    11881 ttgatgcaaa tactttttcg ccaaaatcgg ttaccatttc tggtctatca gtacatgctt
    11941 gtgctatcaa tttatttaca tcttcacgtg tcataccata tggaaattct ggaggtcttt
    12001 gtacaaatga tggtgcagca gctgcagcta ataccaattt tgacactcca tatccattaa
    12061 ataaactcat atacctaagt acgattgctc ctcccattga aaaaccaaca agggtaaagt
    12121 cttttaatcc aatagcatgt accaccttat aaatatcgtc tgctaattgg ctataagtat
    12181 atcctcctga tgttgcatca gatttaccaa aacctcttaa atcaattgaa actgttctat
    12241 atccgagttt tggcaatata tttgtttgat attcaaatat cttatgtcct aagggccaac
    12301 catgtataaa aagtacagtt tttttagcac tcggattcaa atcatatact gctatgttta
    12361 tatcattcac atatacataa tacatatcaa cagcaactcc cttcaagaaa atgtatgatg
    12421 gttattttat atatatgaat aaaatgatga ataactaaat tttcaaattt atttacaata
    12481 cttctctatt gccttttcaa ataaacttac atgaagtttt tcatctagga ttattctatt
    12541 taatatagct tttatataag ggtcttcaat aagacttatg tctttataat aattattaat
    12601 agccacatgc tccaaatgca aatctagttt taatctgtta caaatagaat ttccataaca
    12661 tacaaaacca ccattccaat atgcaccaca agaacttgta gaacctctat atacaggatt
    12721 tccacccaat aatcttatag tctttgctaa tatctccata tgtaacattt ctgttataga
    12781 tatatttatc cacatttcac ttaactctct atgcgtttca tctaaatcaa aattatggta
    12841 tagatattga tttacactcg taaattcact tgatactccg gcatagtcat ccatcaataa
    12901 ttctgcatag tacttatttg gacctaaaac ttttatctct ggatatggct catcacttga
    12961 atatccattt cgcttgtgca tgtcataatg tgcatcactc ataatatctc ctcctttaaa
    13021 tttaatgtat gatattaatt gtatgcatca caactttttc atataactta gataataaaa
    13081 tctacttaaa taataaaaag ccatattaat taaaattaac atgacctttt atccacaaaa
    13141 ttacttttta attattcatc ttttccagct actgatagct ctttaataac aactgaaggt
    13201 gaacctatac tactcattgg aaattctaaa tcttctccta tagcttcaat atctttcaac
    13261 aggtcaaaat agtttcctgc tactgtaatt tgctccactg gaaatacttt ttttccttct
    13321 ttgatataaa agcctttggc agctaaagaa aagtctcctg ttactgaatt tgcacctgaa
    13381 tgaagtcctg caaagctagt aaccatcaat ccttcatcta tatcctttat aatttcatct
    13441 aaagatttat ctcctttctc tatataaaaa ttagttggat atatgcttat aggtgatgaa
    13501 tatgaagatt ttattccatt tcctgtagtc tttacacctg ccttattagc cgtttttaag
    13561 ttatgaagta aagttataag ctttccattt aaaacaactt cttttttaaa tgtagctact
    13621 ccttcatcat caaaaggagt agatgccata ccattttcta aaagtgggtc atcaactatt
    13681 gtaactatag gagatgctat catgtctcct tctctatttt tcaataaaga taatcctttt
    13741 tgagctgaat ctgcattaaa tatcccagaa aatgtgctaa gcaaagatac catagcttca
    13801 ttaagcaata tagttttata tttacctgat ggaatactct taccacctac tttagataaa
    13861 gcattatcaa caccaagttt tgctatctca caaggtttta tttcttcaat agaatttgct
    13921 atattatatc ctattccatc atatttttgt ccattgtctt ctatgatagg aactacaaac
    13981 cctataagca tattagtttt attgcttaaa ttaagacctt tagtattaga tataccatta
    14041 cttgatacag aataagatat agtacattta gataaattta ccaccttatc tgaataagct
    14101 ttagtctctc tttccatttc taaagctaaa tcaatcaact tatctgcctc taaattttct
    14161 agctctttag aataagtctt tacatcatta tagtgcttat ctccttcata tataaattgt
    14221 acatcttcat tttcaatagt gtttactcca tcttttacat tttttatcaa catatcaaca
    14281 gctttatcat ctaaaatttc tgtataagaa taacctatct ttccatttac cttacctcta
    14341 aaagataaac caaaagattt atcaagattg tatttctcaa cttctccttc atatatattt
    14401 atacttaaat tttctcctgt ataataatat atttcacact cttcaaaacc ttcacttaga
    14461 gcctttttaa ataatatatc tttaaaattt ttaagttcca caattatccc tcctatcttc
    14521 cacctacagt aatttctttt acccttatca taggttggcc tacattagtt ggtatacttc
    14581 ctgatgatga gccacacatc ccctgagctt gttttacgtt attcccaatc atatctatat
    14641 tcataagtat atcgctacct tttccaatta aactagcacc tctaactggc tcttgtattt
    14701 ctccattttt tattaagtaa ccttctgaaa ctgcaaaatt aaactctcct gttacaggat
    14761 ttacagaacc accacccatt tttttagcat aaagtccatc tggtattgac tttataatat
    14821 cttctggttt atcctctcca gcagcaatat aagtgttagt catccttgaa gttggtgcaa
    14881 atttgtagtt ctgtctacga gaacttcctg taggattcat tcccattcgt ctaccattaa
    14941 gtttatctat catataagac ttcaaaattc cattttcaat cagtatattc ttttgagttg
    15001 gatttccttc atcatctata ttcatagaac cccagtagtt tggtattgta ccatcatcta
    15061 tagctgtaat ctttgtggaa gcaatctgtt gacctaattt atctgcaaat acagagttcc
    15121 cctttgcaac tgatgtagct tccaaagaat gtccacaagc ttcatgaaat attactccac
    15181 caaatccatt atctattgct accatcatat tacctgcagg acaattttta gcatgcaaca
    15241 tagtatgtgc tactctagct gattcttttg cataatattc tgggtctatt tcttcaaata
    15301 tttcaaaccc tttacaacct ccaggacctt caaatccagt ttgattttca ttacccttag
    15361 atgcaattga acttatccct agtctagttc ttattcttct atcctcaaca tatatacctt
    15421 cagtgtttgc aattaatatt ttttggtctt tatccaaata actaacactt acttgagata
    15481 tatcattact aaattctttt gcactcttat atgcaacctt cataacactt actttatctt
    15541 tatttcctat tgaatttgga taaattttta tattatgtat gttatttatt ttaatactgt
    15601 catttaatat tacacttttt ccatcttcca atttacctaa agcaacagca gctttgtatg
    15661 ctacatccaa cagactattt aatgaattgt cacttgtata agcatatata cttttcaatc
    15721 ctttaaatat tcttattcct ataccataat ttcttccacc tattgcattt tctactttat
    15781 tatctattaa actaatagaa ttatttatag tgtcttcttc aaaaatctca gcaaaatctc
    15841 ctccagtaat tagacacttt tctaatacct tagaagctat attttttgaa agcataaatt
    15901 tacctcctac tttaaatttt taattaactt ttattcaatc tttttacttg ttaacaaaca
    15961 ttatatacta tattacttaa aataaggtaa ttatgaatat attatacata tgttttatac
    16021 taaaatgtta tcacatttta tctatacata cccatataac tagataatta aaatgtataa
    16081 tattatttgc tatagaaaat aatacactca tacataataa attttaggag tgaagtatat
    16141 gaatcaagaa tatgtaaaag gaaatcctat ttttactaat attaataaag ttccaagaca
    16201 atttccatac cttactgatg atattgatac tgatgttata attgttggtg gtggcgtaac
    16261 tggatgtatc tgtgcttact atctagctaa aaacaatata aagtctgtca ttcttgaaaa
    16321 aggaaggatt gcacatggaa gcactagcgt gacaacttct ctattacagt atgaattaga
    16381 tgacaatttg atagatttaa cagaggttat gactctaaaa gatgctctaa aagcatataa
    16441 cctttgtact tcagcacttg aagaattaga tacatttata gagctatatg gaaataagtg
    16501 tgattatgcc aaaagagata ctctacttta tactgccaac aaacttgaag ttaaggctat
    16561 caaagaagaa tataacttaa gaaaagaaaa tggatttgat gttgaatata ttgatgaatc
    16621 cacaaaccct tttagctttg atttaaaatc aggattgata gcaaagaatg gaggacgaga
    16681 attagaccca tataaatata gtcaccattt gattgatgta agtttaaaaa atggtcttca
    16741 agtatatgaa aatacagaag taaaaaaagt agatttatca aatgataagg ttactgctga
    16801 agtatcgtat ggatacaaag ttcatggtaa aaaattaatt gtagctacag ggtacaatac
    16861 aagtttattt acaaaaagaa attttgcaac taagtcaaat acttttaata tcgcaacgaa
    16921 accacttaaa aacatagctt cttggaaaaa caacatttta attagagata actgtgaccc
    16981 atataattat ctaagaacta ctaaagacaa tcgattaatt ataggtggtg aagatgtaag
    17041 ttttgatgat atagaaaatg aaactttagc caatgaaaag tacgatattt tagaacaaag
    17101 attgaaaagt atgtttaagg atataaaaga cattgaagtt gaatataaat attgtggttg
    17161 ttttgcttca actttagata atttaggttt tattggtcca gataataaaa ataacaatct
    17221 atggtattgc ttaggttatg gagcaaatgg tatactcttt gcaatattag gaggtataat
    17281 gttaagtgaa ttatatcttg gcaaacaaaa taaaaatatg aaattattta aagtagatag
    17341 atttgataaa tagatattat atattaaaag actgtaaaaa gatgaaactt tataattcta
    17401 aaagttaact ttgatacagt cttttataat gttcctttta aaaatacttt atttataatt
    17461 aaatataaag taaaatgtta aaatataact aataaaattt agtcataaga taatatttaa
    17521 gtttatttga tgaaatatgg aggaattatt atgaaggaaa tactcgttat tgctcccact
    17581 aaaggaacct atgaaaaatc aattcacata gttaaaaaaa acaaatatac aaatattgat
    17641 gttgtatttg gcaatctaaa agaaggtata cctcttgctg aaaaaagtat taatcatggt
    17701 actagaatta tcataagccg aggtggaact tacaatatgt taaaagctac ttacaatata
    17761 cctatagttg aaattaaggt agatgcttat gatattataa aaagctacaa agaggtaaaa
    17821 aattccaacg aaccatttgg aataattgga tttaataatg tcatctacgg tttcgatatt
    17881 atagaagaaa tattgaataa gaaaataact atgattgaaa ttgaaaaaga agaagaaatt
    17941 tatgatgcta tagaaaagta cagaaagaaa ggtataaaca catatatagg agataccact
    18001 gttgcacata ttgttaagag acttaattgt aagggaatat taatcgaatc tagagaagaa
    18061 aacatactta gagccattca acaagcagag caaatactcg aagcaaccaa ggatgaacaa
    18121 aaaagaagat tacagataga agctatgaca gattttgtac atgatggaat aataacagtt
    18181 gataaaaatt ttaaaataac attatttaat aagagtgctg aaaaaatatt tggtataaaa
    18241 aaagaaaatg cactttatca taatgttgta gatgttatcc caaacacagt tcttccacaa
    18301 gtagttgaga ctatggaacc tcaaattggt gaaatacaaa atatagggag tacaaaaatt
    18361 attacaaata gagttccaat cattgttgat ggtcagattc aaggagctgt ttcaacattt
    18421 caagatagca aggagataag ttcttttgaa catacaataa gaaggagctt attaagcaaa
    18481 ggtctatctg ctaaatacac ttttgatgat ataattcatg ctagtgaaaa gacaaaagat
    18541 tgtatagaac ttgcaaaaaa atatgcttgt tatgatactc ctatgattat tactggagaa
    18601 tctggagttg gtaaagaact attttgtcaa agtatacata actatagtaa gcgtaaaaat
    18661 gcaccttttg ttgctgtaaa ttgtgctgcc atacctccat ctctaataga gagtgagttt
    18721 tttggttatg aggaaggttc atttacaggt gctagaaaaa aaggtaaagc aggtgtattc
    18781 gaactagcac atgaaggcac aatcttccta gatgaaataa gtgaaatccc tcttgaactt
    18841 caaggtcgat tattgagggt attgcaggaa aaacaagtta tgagaattgg tggtgataaa
    18901 gttattccta tagatgtaaa aattatctgt gcttcaaata aagacttaaa aaatatgatg
    18961 cgcaatggtt tatttagaca agacctttat tttagaataa atatactaac attatatctt
    19021 ccttcattaa aagaaagaaa agaagatatt ataaaattat cagaattttt tatacataaa
    19081 tattcaagca aatacaataa aacgccttta attatcactc caaatattaa aaaggcactt
    19141 cttgaacgag aatatgaagg taatataagg gaattggaag gtatgatgga aagggctgtt
    19201 attgtggaat catttgactc gattttatta gaaaagctta gcaatgaata tacaaataat
    19261 atagttgata aagataatag tgattttgaa tgcagtaatt tagatttaaa aactttagaa
    19321 aaaaaatata tctataaaat ttataacgaa aataatagaa atactacaaa aacctgtgaa
    19381 attctaaaga taactcgctc cactctatgg cgtaagttaa aagaaataga agatgatgtt
    19441 taaaaataat acatatctat atatatcaaa acgttttatg tttaatttta atacataaga
    19501 cgtttatttg ttttcaataa aaaatacaat tatatgataa aaagaatctt tgtaagtgaa
    19561 attctcgtca atcatacttt taattataag taatctacag catttcaatc gattattaaa
    19621 aataatcata ataactaaag atacaaactc tatatattgg catagtaatt gcttttataa
    19681 tacctatata aaaatgaaag ggagtgatac tagatgcaca tcttaaaatc agtgaaaaag
    19741 ataccaggag gacttatggt ctgtcccttg ttatttggag cactatttaa tacagtatgt
    19801 ccacaagctc ttgaaattgg aggattcaca acatccttat ttaaaactgg tgcaatgagt
    19861 atacttgctc tattttgtct atgcaatgga gctcaaatca atatcagaca agctggtaaa
    19921 ccattgacta aaggtatagt acttacagca acaaaattta taattggtgc agtattaggt
    19981 attattgtta gtaaatttat tggacctaaa ggtataattg gtattacacc tcttgccata
    20041 gttgctacca tgactaattc aaatggaggt ctatttgctg ctcttgcagg tgaatatgga
    20101 gactccacag atgttggagc aatatctatc ttatctctaa atgatggtcc tttttttaca
    20161 atgcttgctt ttggagtaac tggacttgca aatgtaccta tagtagcact atttgcagca
    20221 cttataccaa tactacttgg atttatactt ggtaaccttg atgaagatat acgagaattt
    20281 cttgcacctg gtacaacatt attaattcct ttcttcgctt ttcctttagg agcagcatta
    20341 aactttaatc aaattataac tgctggacta cctggagttg tacttggttt aggagttact
    20401 ataataacag gtatgggtgg gtactttgta atgaaactta tacgtgcaga acatccagca
    20461 gtaggtgcag ctacaggtac aacagccgga aatgcagctg gtactcctga agctttagca
    20521 gctatagacc ctactctagc tacaatagca gctgtttcaa cagtacaagt tgctgctgct
    20581 attattgtta cagcaatatg ttgtccaatg ttagtttcct atctagataa aagagaaaaa
    20641 aagaaaaaat ctatttttaa aacaaataaa aatattgaag taaattaata cttaaggagg
    20701 taaaattaat gaatgcaatt attcatgcaa atgaaaaaga taatctagtt acatgtgtca
    20761 gacctttaat taaaggtgaa aaagtttcaa ttgatggaaa gtcttacata gtaaatgata
    20821 atatacctgt ctttcataag atggctacag aagatgtaaa aaaaggtgat gttgtataca
    20881 aatacggtga aattatagga attgcaacag ttgacataaa aactggtgac tatgttcatg
    20941 tacataacat tgaaagtaca cgtggtcgtg gagataaaaa ataggaggaa gttaaaatga
    21001 aattaatggg ttatttaaga gaaaatggtc agtttggtat tcgtaatcat gttttaatta
    21061 ttccaacatc tgtatgttca agtgagacag ctacaagaat agcatcactt gtgcctggag
    21121 caatagcaat ccctcatcaa catggatgtt gtcaaattgg ttctgatata gaacttacag
    21181 caaagacatt aataggattt ggaaaaaatc ctaatgttgc cgcagtttta gtagttggtc
    21241 ttggatgtga tggtattcaa gcaaaagaat tagcatctga aatagctaca acaggaaaaa
    21301 aggtagatta cgttgttata caagaatgtg gtggaacatt aaaaacagtt tctaagggag
    21361 ctgaaatagt aagtaaaatg gctcgtgaag tatcaaaaga agtaagagtt gaatttggta
    21421 tgagtgaaat tacattagca cttgaatgtg gtggttctga cccaacaagt ggaatagctt
    21481 caaacccttc aataggcgtt gcatcaaacc ttcttgtaga tgaaggtgga agtagcatac
    21541 taagtgaaac tacagaagta attggtgcag aacatttgtt ggctacccgt tttgaagatg
    21601 aagaaatgaa agataagttt cttaaatttg taagtgatgt agaaaaaaga gcaattgcta
    21661 tgggagaaga tttacgtagt ggtcaaccta ctccaggcaa taaagctggt ggactttcaa
    21721 caattgaaga aaagtcactt gggtgtatgt ataaggcagg caataagcca ttcaaaggtg
    21781 ctcttgaata tgctgatatt gtacctcctg ataaaaaagg attatatttt atggatactc
    21841 caggtcaaga tatagattct atcactggta tggtagcagg tggtgcacaa attgtaatct
    21901 tttcaactgg tagaggtact ccaacaggaa gccctatttc accagttatt aaaatcactg
    21961 ggaatagtga tacatacaac aaaatgccag acaatatcga tataaatgca ggtagaatta
    22021 taacagatgg tgctaaaata gcagatatag gtcaagaaat atttaatgaa ataatagaag
    22081 tctgtaatgg taaacatacc aaagctgaaa gtcttggtca tagagaattt ggcatttaca
    22141 gaatttcatc tacattttag attgtaacaa aatatctttt ataaatataa tacttatgta
    22201 gatacataag ctaaaagttc tttctaatcc tataaaaatt ttctatactg aaaaggaact
    22261 gtatcaaaat tattttgaaa atagttcctt ttttcacctc ttttactcaa aatattataa
    22321 aataagattt aaacaataaa cgtatcttga ttaatctcta agaagacata aataaagcta
    22381 aatgtaaagt gagataaata aaatttaata tctttgtatg aaaagatatt tggtataata
    22441 tgtctgagag aattcttatt tgtaaacaaa taaaggatgg aggatgaaac aaaagaatga
    22501 actttgaaaa cacaaaaaat aaaaaaataa tagctacttt actattgaca ctccttttaa
    22561 tcgtgtcatt aacatgtgtc acttttatta tgaatctaaa taaagtaaca cataatgaaa
    22621 cagaacgata tcttgtagaa gtttcacaaa gtatttccac tacaattaat gtgaaatgta
    22681 aaagtatatt taggacacta tattctacgg gagatactta ttttgagatt aatgatagtg
    22741 aaaaagaagt caataaatac ttatcaaaaa aattacaatt atttgaattt gattggttag
    22801 gtattataga ttcaaatgga aattttataa gtgcccttaa agatacaggt aatgttaaag
    22861 gacaagactt ttatagaaaa gcccttcaag gtaaagaaac aattactcct gttaaatcac
    22921 aatctggtac atatggaatt ttatattctg taccactata caaaggtgat aaaattgttg
    22981 gagctgttgc tgcttggaat actattgata ctatgagaaa tttattaagt gtagaaagct
    23041 ttcatggtga aggattttcc caaattatcg accaagatgg aaattttatt gtaagttcag
    23101 caaacaaaaa tgctccaaaa aatgtggtga atttttttga catgattaaa caccaaggaa
    23161 aacaggttga taaaaataaa cttgaagaaa tgaaaataaa cttaaaaaag aacaaaactg
    23221 gttctatata ttacacatta aatgatggta tttctaaggc aatgcactat cttccattac
    23281 aagaagaaaa tttatacctc ctttcagttg ttccaacaaa aacagcaagt gcacagtttg
    23341 atttacttct aaaaaaagca atcttcatca atattttaat tgttatacta tttttattgc
    23401 ttattatatt gattatgttt ataaatcaaa aaaacagggc acaacttata aaaatcgcct
    23461 atgtagactc tgtaactaaa ggttacagta aagcacgttt tgatatggaa gctaaaaaaa
    23521 ttatacagtc atcttcatca gaaagctatt cattagtatc tataaatatt cagaaattca
    23581 aacttattaa tgattctttt ggtagtgaag caggagataa aacattaaag tacatacatg
    23641 atgtcattag taattatcta gaaccaggag aattactttg tcgagtttct aatgaccagt
    23701 ttaatgtact tattaaaact attcctcaaa aagaaattct taagtacata gaaaatatca
    23761 ccaatgatat taatagtttc aataaaaatg aagaatgtaa atactttata gctctatcta
    23821 taggtgtata taaaattgat gacccaaatc tacctttagt ttcaattcaa gaccgtgcaa
    23881 atgttgcacg aaaaaataat aaaggaaaca ttggaaatca tccactttat acatgtgttt
    23941 tttatagtga tttagagcgt ttaaaaatga ttaaagaaaa agaaatggaa aatagaatgg
    24001 aggaagcatt aaataataac gaatttatag tatatcttca gcctaaaata agcctcaaag
    24061 ataatactat tgtaggtgca gaagcattag taagatggca agacccaaat aaaggtctta
    24121 taccaccaga cgagtttatt cctttctttg aaaaaaatgg ttttattaca aaactcgacc
    24181 actatgtttt tgaaaaagta tgcagtatga taagaaaatg gatagataat ggagaaactc
    24241 caatacctgt ttcagttaat ttttcacgtg ctaatttaaa taatatagat tttttagaaa
    24301 aatacagaat gcttcgtaat aaatacaaca ttcctgctaa gctcttagaa attgaattaa
    24361 ctgaaacact tgtatttgaa aatctccaaa aattaattaa tataatagac caaatccatg
    24421 aagaaggttt tcaatgttca ctagatgact ttggaagtgg atactcttca ctcaatatgt
    24481 taaaagaaat aaaagttgac acattaaaac ttgaccatgc tttttttagc tcacctaatg
    24541 cagataatat tgctgaaaat catgtaatag aatctatagt taatttggca aaaaaattaa
    24601 atatgaatag tatatcagaa ggtgttgaaa caatcttgca aatggattat cttaaaaaaa
    24661 ttaattgtga tatgatacaa ggatatgttt tttcaaaacc tctacctcaa gaaatatttg
    24721 agaaaatgac ttttggaaaa aaactagaag aaaccagtat ggatagttcc gtttaaataa
    24781 aataaaaaac ttgtctctat aaaataattt taatcatttt ataaagacaa gtctttttat
    24841 tttccaattt aattttattt tctaaatttc ttacactcgg cttgttttaa attccctttt
    24901 taagaataat aatacgatta tcatagcaat aaaatctgca actggctgtg ccaaccaaac
    24961 accatctaaa ttaaattgaa ttggaagtac aatgattaat ggtataaata aaataaattg
    25021 tcttaaaaga gttaaaaaca ttgaatgttt aactttactt atacactgaa aatacacagg
    25081 tcctacaata gaaatcccca ttataggcaa tgtaattaga tttaggctta tacctcttac
    25141 tgctatatcc atcaaatctg aatctttatt aaatatacct acaaatactt ctgggaaaac
    25201 ttgtactact aaaaatccaa atgacaatat gacaactgac actatgatag ataatattaa
    25261 ggttttcttt gcacgctcat attgttttgc accataattg tatgatatta tagtttgcat
    25321 accttgattc aatccaaaca caggcatcat aaacatcaat aaaatagatg taagtgctgt
    25381 catagctcca atagctaaat ctccaccata aacctttaga gaattatttg taaacatgtg
    25441 agttatactt atagctactt ccatagcaaa aggtgtcatt cctattgcaa ataccttctt
    25501 tactatatct ttattcaatt ttaagttaca tttttttagt tttaaattgg acttccctct
    25561 tataaaataa tgtattaccc aaaaaaatac tactatttga cagagtactg ttgctatagc
    25621 tgctcctttt atacctaaat taaatacaaa tataaatata gggtccaata tcaggtttaa
    25681 tacacatcct acaatcattg tccttgctgc taattttgga ttaccttctg ccctaattgc
    25741 attattaaga gaaaatgcaa ctaaattaaa tatacttcca accaatatga cactcatata
    25801 gtcttttgca taaaatattg aatctttact tgcacccaaa ataaaaagta tctcttctaa
    25861 gaaaactaat cctaatatag ttataattaa agagataatt atagataatg tcaaggcatt
    25921 acctaatatc ttctctgctt cttctctatt tctttcccct aactttatag acaatcttgt
    25981 gctagcacct actgatacca acattccaaa agctattatt agagtaaata ctggcatagt
    26041 tacaccaaga cctgcaatag ctatagagcc aataccttcc atagaaccaa taaaagctct
    26101 atctactaca ttataaagtg aagttaccat cattgctatt attgcaggta tcgaatacct
    26161 aaataataaa atccatattt tttcatgtct taatgcttct tgattctcca tgttgtcacc
    26221 atttacacca tgtcataagt tatacaaatt ggtttatttg ttcttgggtc tgttgctatt
    26281 tctgcatcaa tattaaataa ttcttttaga ttatccttcg tcataacatc atatgcatca
    26341 ccttcacaaa ctattttacc tttcttaata cctatcatgt ggtcagcaaa tcttgctgca
    26401 ttattaagtt catgtattac catgacaata gttcttcctt gttttttatt tagttcatct
    26461 aataatttta gtatctccaa ttgatgtgcc aaatctaaat atgttgtagg ctcatccagt
    26521 aataatatat cagtttcttg tgccaaagcc atagctatcc atgccctttg tctttgtcct
    26581 cctgataaat cttccatatt tctatcctta aattctgcaa ttccagtaac ttctaaactc
    26641 caatttacaa tctctctgtc ttctttacta gaattaccaa atccactttt atgaggaaat
    26701 cttccataag aaattaattc tgaaacggta agaccactag gagcttgagg gctttgtgga
    26761 agtacagcca tatcttttgc tatttcttta gacttctttt tgtagatgtc ctctccattt
    26821 atatatattt tcccactatt agggctaata attctaccta tagttttaag tattgtcgat
    26881 tttccacaac cattagcccc tatgatggta gttatttttc cttttggtat atttaaattt
    26941 aaatttttta taatatctgt gtttccatat gatacattta gattcttagt atttatacag
    27001 ttcataacaa caccctttcc taatcattta ccaacattaa ataaataaag tacggcactc
    27061 ctattataga aactactatg cctactggta tttctatagg tgctagaaga tttcttgata
    27121 tagtatctgc tattaaaagt aataatgttc ctatcaatgc tgatactggt attaatcttt
    27181 tatgctttgg tcctattatt tttctaccta tatgtggagc tattaaacct aaaaatgaaa
    27241 tactacctgc tactgctgtc gatacaccag ataaaattac tgcatatatt ataagtcttc
    27301 ttctttgttt ttctacatct acaccaagac ttatagatac ttcatcaccc aaatttaaaa
    27361 catctatata tttagattta taaatagtta tacccataaa aatcactata aatggaagca
    27421 cagctattac atatttccaa ctggttcccc aaatgcttcc tgatgtccat gccataaccc
    27481 tattaaattc ttgtgttgta aatttcaatt ggaatatcgt caaaattgaa gtaaaagcta
    27541 tgtttacacc tataccaata agtagtaatc ttgacgaatt tataccacct ttccatgcaa
    27601 gagaatatat aagaaatgct ccaaataaag ctcctataag tgcaacaata ggcattgtaa
    27661 atacagtcaa gttactcata ccttcatata cattaccatt cataaggtat atataaacta
    27721 ctacaaacaa tgcagcacct gaatttattc ccaaaattcc agaatcagct aaatcattct
    27781 ttgtaactcc ttgaagtatt acacctgctg ttgataatgc agaaccaacc aaaagtgcta
    27841 ttactattct tggtaatctt aatttaaata ttgcaatttc atgattacgc tgaccctgcc
    27901 ctaaaaaagt ctttataaca tccattggtt ctatagaaaa tgagcccata tttaaactca
    27961 ataaaaaagt gataaaaata agcaccatta aaattgatat aacaagtaat gttttagaat
    28021 ttatttttat aatatttttc atagtaccta tttctccccc tttctaacaa ggtatataaa
    28081 tattggcact cctataagtg ctgttataga acctactggt gtctcatatg gaggatttat
    28141 catcctactt aatatatctg tatatacaag caatacagct cctaatacta aagagctcgg
    28201 tattatatac ttataatctg aacctactat tcctttagct atttgaggaa ctataagacc
    28261 tataaaaata atattaccag acactgacac agatgctcca gtcattaata ttacaagtac
    28321 tatacatata aatctaacaa agttagtttt ttgtcctaat cctattgcca cttcttctcc
    28381 aagacttaat atagttattc ttggagccat aataatagct aaaataaatc caatacctcc
    28441 agctataaat agcaatttta taccttccca cttcactcct acaagccctc ctgatatcca
    28501 gaaactcaac tgttgagata ggttaaaata catagatatt gctgatgcca aagaaattaa
    28561 caatgtacca agagctactc ctgcaagtgc tagttttaca ttattaactc ttcctcttga
    28621 tttagaactt ataaaatata taaataatcc acttatactt gccccaataa atgcaaacat
    28681 cattacacta aaacttccat gtataagttg aggaagtttt ttttgaagta ccagtgatat
    28741 cgaaattgca aatgtagccc cttgtgtaat tcctataaca gatggttcag ctataggatt
    28801 tctcgttatt ccttgcatta ttgccccgga aactgctaaa aaacctccaa caagtgcagc
    28861 tgctattgcc cttggaagtc taacatcctt tactattctc atatttattc catcttccat
    28921 ttttatcaag ctatctataa cagttgatag attcatatct tttgccccaa tagttatgga
    28981 taatacaatt ccaaatacaa gtacaactat acttactaat atcaacaaat aagttatatt
    29041 ttttttactt aacttagtca attatcctac ctccttttcc cttttaatcg tcagtataat
    29101 atcatctatt aagtatttta attttaagct ttattaaaaa taggtgtgaa aaatgaatat
    29161 cattatctca cacctactaa gttaagaatt actttgttaa ttcattttta actgattcta
    29221 ataataactc ttttccaatt ggattgtatg attgactgaa aaatggagca gcgtcaagta
    29281 ttgttacatt gccatctttt actgctctta tttgagccca gatagcacta ttttctaaat
    29341 cttttttatc tgattctgta gcaattacaa ctatatggtc tgcatctata tctgttaaac
    29401 cttccatact taccattggt agtgttattc catcctgttt tggcatattc ttaggtctag
    29461 ctaatttcat atcatcattg attagagtac ctattccacc atcgctaaat accataaatt
    29521 gacctgaact tgccaacact ggtaaataag ttttttctcc attcttttct ataacttcct
    29581 tacctaactt tgtagctttt tcatcatact tctgtagcca acttttagct tcttcttctt
    29641 tgtcaaataa ctttgaaaca tcagttaact tgcttctcca gtcatttgca taatctttca
    29701 tcataactac aggagctatt tcttttaatt ggtcatatat tttttcttgt ctttgactca
    29761 ttattattaa atcaggattt acttctaata tagcttccat atccatagta tccatcattg
    29821 agtgcccaac tatctttaca cctttaagct cttctcttat gtaacttggt aatttatcag
    29881 tttcatatga gtctacattt gctgttgcaa caggcttata acctgctaat aatagttctt
    29941 cactacttcc tgatatatca actatttttt taggatttga tggtatctta acctctcctt
    30001 taacagattg tacaactctt gtctctgatt tatctttatc ctctgtattc ttatctgaac
    30061 atgctgtaac aagtactaat gtagttataa tagatataaa tattgcaagt gattttattt
    30121 ttttcatgat tacttctcct acttgtttat tatacttaca tgatattgat tatcataagc
    30181 acatatttat tatagcattt ttctattttt agtcaatatt attttgtaaa aggataaaaa
    30241 ttcctaatta atcttaatta tatatagctt agataaggtt ttcttatatt aaacagtatt
    30301 tttttaaaga attggacaca aatacaatga cttttaggtt aaacaatgat aaaatgcaat
    30361 ttatagataa tgaatttagt aaggaggatt caaaacatgg tacatttagt atattgtgat
    30421 aatgcaggaa aaaaaggaga aaaggtctta gataaaatct tggctggaac aaaaacaatg
    30481 gttattagag gtgctgctgg tagaaaaata ccacatagta gagtatttga agatgagtta
    30541 ctttatttta tggaaaaagg aactgcaaaa atatctgcaa aagccactgt taaaaaggta
    30601 gaaaactatg ttaaattaac agaagatgaa atttctaaaa ttttaaatga aaatcaacca
    30661 aaattaaatt tatctgaaaa gcaaaaagtt cgttggcata aaaaatgttt atgcttagtt
    30721 gaatttgaaa atgttgaaga aataacacct cttgattttg accatcaagg aaatatggat
    30781 gattggctta ttgttgaaaa aattgaagat gtagttgttg gtactagtat accttacaat
    30841 tatgagaagt ctaaatttta aagtatcaat aacataatac tatattgcta aactctactt
    30901 aaataaaaaa agagtgcatt taataattat aaaaattatt tttatacact cttttttaat
    30961 aataatttta aattacttac tttctaatct ttcatataca tctataaatt cttctgctaa
    31021 ttgttgaaaa ttcatagctg tcattaaatg gtcttgtgca tgaataagta taacacttat
    31081 ttcagtttta ttaccattag cttcattttg tatcaattct gtttggaatt tatgagcttt
    31141 atgaattgat tctttcgact tttcaataca ttctctagct ttatctatat tcccgctctt
    31201 tgcttctttt attgcttcaa atgctagccc tttcgcatca cctgcaaatc caattattcc
    31261 aaaagatatc tctatcatct tctcatccat ttattcgacc tcctaaatat ctctatagct
    31321 tgatatgact aatccattct tttctaaaaa ttctttgact tttttagatg taagtatctt
    31381 gtgctctttc gttctatcaa tagcataaga tgtagaattt aaaatataat catctaaaaa
    31441 agctgggtga gacataatat ccactacatc atacttcatt atttcgtcta tattttttat
    31501 aaaatattct tcacttacat tttccttata aaaactatct attaaaggaa ctactttaga
    31561 atactctaaa ttatattcaa atccacctct tattggaaga ttatacttgg ttacaatctt
    31621 ctctattact ggctttaaac ttactaaagt atggatatga tggtgactat ctaaatgaga
    31681 tatatctatt ccaagccctt ttaccctatc tatttgagca caaagttctc tataaatctc
    31741 atcacaatcc attttttcaa taatttcgtc tgtaatcctc ctaataaaaa aaccgtcttt
    31801 atcaacaata gtttttaagt tagataataa tggtctacca caacttaaag tcatatgaac
    31861 tccacaattt aaagttttat tttctttaag taagcctact ccatgttcta ccccaggcat
    31921 atttgccatc atagaagtag acctaactat accattatta tgagctgaaa ttattccata
    31981 gttcacagct tcacaatatc caaaatcatc tgcatttatt ataattttag tcataccata
    32041 acctactttc tatagacaac tatctattaa atctaggaag ataatcttta tgagcttcca
    32101 acatttcgtc taatatagtt tttgctaagt catcagatgg aatcaatggg tttatacaaa
    32161 gtgccaaaag agcagattca taatcgccat atacagctgc tttagctgcc aatacttcaa
    32221 atgatttaat ttgacttact aatccatgca cagaatctgg taaatatcct atacttattg
    32281 gttttggacc attttttgtt attacactac ttacttctac cgcttgttca tctttaaaat
    32341 ctcttataga acccttattt aatgtattaa ctacttggat atcttttttg tcgttgtata
    32401 tagaacttat taaattacaa gcagcatcac tatagtaagc ccctcctctt ttttcaagtt
    32461 gaggtggttt tacatctaat ttttcatcct tatataattc aaataattgt tcttccaatt
    32521 ctttaactac ttgacctcta gcttttccct ctttaaactc tctcaactca tcctcaagca
    32581 tttctttagt tttatagtag tatctatgat atggacaagg tattgcaccc aaagatttta
    32641 caaattcagc attaaaatca attgccttta tattttgcat acttatatct gctttgacaa
    32701 atttatcaat tgcttctttt gtaacatctt ctccatctaa agatacattt agaccataaa
    32761 ccatatgatt aagacctgca aaatccatac ttattctgtc actttctaca ttaaatagtt
    32821 tggcaacatc attttttaaa tgaattggca cgttgcatag acctatatat cttctaaaat
    32881 tagtatattt gaatacagcc tctgttatta ttcctgttgg atttgtaaaa ttcaccatcc
    32941 atgcatttgg acataattct tccacatctt ttattatatc aaatataact ggtattgtgc
    33001 gaagggcttt aaataaacct ccagcaccat tagtttcctg ccccataact ccatgagata
    33061 aaggaatagt ttcatctttt attctcgcat ctaaaagacc tactctaagt tgtgttgtca
    33121 caaagtctgc atctcttaga gcttctcttc tatctaaagt aagatttatt ttcatatcta
    33181 ctccagcttt ttttaccatc ctcttagcta gatttccaac tatctctaat ttttcttttc
    33241 cagcttctat atctacaagc cataattcct ttacaggtaa ctcttcatat ctatttataa
    33301 aaccctctac aagctcagga gtataacttg aacctcctcc aattgtaact atttttagtc
    33361 cctttttcat ctcattatac catccttacc tataatttaa tatactctac aaaaccccat
    33421 acatttattt aaaactctat acatttattt accgaaataa aactacctag ttgttaattt
    33481 tcaatttatt agcttccatt ttttcagacg ctatgacaaa tggaatatat attaatattg
    33541 ataatattaa attaaatagt gctaaagctc caccagtcca gtcacctgta gccattgctc
    33601 cacctaatat aggaggtgtt acccaaggta ttttaggtaa aattactggt gctactaatc
    33661 caaaatctat tgctaagtat gatacagcac tacatattac tggtgcaagt acaaatggaa
    33721 tcataaatat aggatttaaa actattggaa gtccaaatat caatggttct cctatgttaa
    33781 atattgcagg tggaccacct aatactatca tctctttacg tcttcttcca gctattaata
    33841 aagctactat taatcccaat accattccag aaccacctaa atatacaaat gcatcaaaga
    33901 atgcacctgc aagtacatgg tatccttccg ttgctcctgc ttgagccaag gcagcatttt
    33961 ctccacctaa tttcataagt atagtttctg taaaaggaag tgctatattt gcaccatgaa
    34021 gtccaacagt ccaaagtata tgaattataa atgcaataag cattgtagaa cctaaactat
    34081 cagctacatt tgatagtggt gctcctaaat aagtatttaa aatatctgtt aagaaacttc
    34141 cattagaaag catttttata aatattccta ttacagtaaa tatcattata gttaacattc
    34201 ctggtattaa ctttgcaaat gatcttgaaa ctgctggtgg aactccatct ggcattttta
    34261 tagttaattt ctttacctta gataatctta caaatatctc agttgataat aatcctatta
    34321 atatagaaga gaataaagct gctgtaccta agtaagtttg ttgtatcatt ccccatcctt
    34381 caactactgt agttcctccc tcaggaacta ttttaccaat ttgtggagcc attataaaaa
    34441 agatagataa agctataagc ccagattgta acccatcact ctcataagat tttgctagaa
    34501 aataagcaac acctattact aagaatactg ccattgtacc tattgctccc caccaaaggt
    34561 cgcctccaaa tgtggtccag ttttctccaa atatacttgc catcaagttt ttgtatgctt
    34621 ccaatggtaa gttatttatc aatgttccca atgcacctac catagttata ggtatcatta
    34681 ctataaatgc atctcttact gctactaagt gcctttgagc tcctatttta gcagctacag
    34741 gcactatata tttatccata aatgacatga atttttccat tttacatcct ccagtacaac
    34801 tttattttga actatttaac tctacagctc tatctaacac agcttttcca ttacatcttc
    34861 cataatccat catatttata acttctactg gaatattata tggttttgcc atttcagttg
    34921 cttttcctaa tacatatcta acttgaggcc ctaaaagtaa gacatcacaa ttttctattt
    34981 cattttttaa gttcacttct gatatagccc atatttttgc ttctataccg ttttcctttg
    35041 ctgcatcctc catttttgta actaataaac tagttgacat tcctgctgaa caagctaaca
    35101 ttatttttat cattttttcc cctccaaatt agattcatta aatatttttt aagtcataag
    35161 ttttttaaac tatacattta aaatatgctt ttctattaat tttttcttaa tgtatatatc
    35221 ttatttttaa atgtatatac atttaattct tgatattatt atataccaaa tgtatataca
    35281 tttcaagaaa actttttcat tttatttaaa ttttattcga caacagcatt caaagttaat
    35341 gtatatccat agtgatatgt gtgtgagtac tcaaaaatct cactattttg taacttacaa
    35401 atttggctta tatataaaag tggctctggt tcttctaatt ttagattatc catacattct
    35461 tcattcgata ttacagcttg tacttctctc tctgacatgg ctatcttgta tccacacact
    35521 tcttcaacaa aattataaag agaattttca caatgagtct tatttaaatt tggaaataac
    35581 ttaactggca tataagtaat catatatgaa actcttgcat cattagctaa tcttactctt
    35641 tttatttccc aaacattttc ttctaaactt atattcaact tacttgataa attttcatct
    35701 gcctgaacta tttcaagaga taccaatata ttagatactt tatagccttt actttccatc
    35761 tctttagtaa atccacttac agacgatata ttatcatata tattatttgc aataactata
    35821 ctacctacac ctctttgtct agttatatag ccttctttaa ctagattatt aagtgcagac
    35881 cttacagtca ttctagttac attaaattct tcactcagct gtttttcaga agggattaaa
    35941 tccccttctt tcaaactgtc tgaatttatt aactctctaa catgattttc tataacttta
    36001 taaattggct ctttcattta attcactcct tattaaatct tttatataat aagataattt
    36061 tatatacaaa caaagaaaat gtctatacat ttaagataat ttaaatttta ttcagaaatc
    36121 ttgtacaact ttccgagttt taataaagat ataaatagac ttatcattaa tatacctttt
    36181 aatatacttg ataaggttat actccaccat acaccattta gacccaacat ttctggtcta
    36241 gatataaagt aagccaatgg tactcttgca ccagtcagta tagtaattat aatagaaggt
    36301 atataggttc tccctaatcc tttgaaagct cctgttgtta ttatttctaa acacataaaa
    36361 aattgagaat agcctaatat tttaagataa actgctcctt tttctattgc ctcactttca
    36421 tctataaaaa tagaaaatat actctctcct gcaaacacta atactaaagt agttattata
    36481 ccaagtacag aagatacagt tatagctatt ttgcaacctt tatttattct agaatacttc
    36541 ttggcaccat aattttgtcc aacaaaactg cctaatgcaa ctgatagccc ttcagccgtc
    36601 atccaggaaa ttgcttctat ttgtgagcct actttttgta ctgcaacagc cacaggcccc
    36661 catgatgcta ctattactcc taatagcatt gagaatactg taaacatacc actttgtatt
    36721 gctataggta atcccaattt atacagaact ttataatatt tagtctctat ttctttaaat
    36781 agtcttaatt taaaatattc ttctttactt ttaactatta tatatataaa acatgtagta
    36841 actactatct gagcaaatac agttgcaata gcagctcctg caacaccaag ttttggagca
    36901 aatccccatc caaaaattaa tatagggtca agtataatat tagttattaa tcctactgta
    36961 ttaatacgaa aaggagtttt actatttcca agaccattga aaattgcagt aaatactgga
    37021 ttaataaaat aaaataccat acctagagct actattatca aatactgtct agacatggtt
    37081 ataacttcta agtctcctaa tctaaaaaaa cctatcaatt gtttattaag tactattaaa
    37141 gaaattgtat atattattgc tagtataata tttatttcta ttgctgactt catatatgat
    37201 ttagtagtgc ttatattatt tgcaccaata ctttgtgcta cttttatctc tccacctact
    37261 tttgaaatca taataaatgc cattgcaagc catggaaaaa aaccagctgt tccaactgca
    37321 gctactgctt tacttccggc tttaccaacc catatcatat ctatcatgtt ataccccatc
    37381 tgtataaatg aagtccccat tattggaagt gatagcttaa gtaatttttc tgtaattctt
    37441 ccttctgtta aattaagtct gtctttcatt ttccacctct tttatattaa atttatttgc
    37501 ttatatagct agattaatca ttttatttga ttgagttcac tatccttcaa tttatattat
    37561 catatctcag gattgcatct caatcatact tatgtttaag atacagtatt ttacctatct
    37621 atgataaaat taatacttat gaatataaat ctagccattt catcaataca tattctaacc
    37681 tttaacttaa cttcaattaa gtatacatat cgtaaccaat tagaatattt tatacggtat
    37741 cttccattaa taaacaacca atatcaatga tttatatata attttattta tacataaaat
    37801 agtatactat aataataaaa aaactatatt tgaatgcttt aagcatgttt actaattgta
    37861 atttccacac aaatttgata atatatgaat atattatggt gaaatttata gatatatatt
    37921 aattatgggg agattgtggg aggttctttt cacaagacat ttaagctatg aaaataaata
    37981 aaaaattcaa tagagttgat atgcaggttt caatacttac tgcaattatt gtaataatat
    38041 cttcctcatg tgtatatttt tttaattata cactaactta taagagcatg atatactctt
    38101 taagtgagag gtcaaaacat atctatgaat atgtagaaag aaatattgat aaaaatacct
    38161 ttactgaaat taattctaaa gaagaccaat caaaaaagtc ttataaaagt tctaaaatcc
    38221 ttttagaatc tgtaaaaaat actactggag tcatgtatct atatactgct aaaaaaacaa
    38281 aagatggatc actagtatat gttgttgatg gtttaaattc atctaggagt gattttagaa
    38341 atgctggtga tttgatagaa aaagagattt ggggagacct taacaaagca ttagataatc
    38401 atatattgtt acctaaaaag attaaatcaa catcttgggg atacatattt atatcgtatt
    38461 ttcctattca caatgatgtt ggtaaggttg ttggagtaat aggaattgaa tttgaagcaa
    38521 aacatcaata caatacattt aaatttataa gtatagttac accaataata gcattattaa
    38581 cttgtctaat atcagcattg attgcagtga ttctatttaa aagaatatct aatcctacat
    38641 atactgattt tgctaataca gattatttaa caggcctaaa aaatagaaat gcttttgaaa
    38701 tagatatgaa taacttaaac aattcaaatc taaagaatct aatatctatc atatcaatag
    38761 atttagacgg tttaaaaaag attaatgata aatttggcca tcagacagga gacttatata
    38821 ttaagcaagc aagtaaaatt atacaatatg taataaataa aaaatatata gtttatcgta
    38881 ttggaggtga tgaatacata atta
//
LOCUS       DAGSRM010000032        37940 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.032, whole genome shotgun sequence.
ACCESSION   DAGSRM010000032 DAGSRM010000000
VERSION     DAGSRM010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37940
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.032"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            40..678
                     /locus_tag="MJE33_001760"
     CDS             40..678
                     /locus_tag="MJE33_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647696.1"
                     /translation="MSNQRKDFDITNDMFQESIPITTKIILEDMPSNDELNHVFSKGC
                     ERKMKKRKIVLITLLLIGVLLLGSILYNLFLGKTANISMLKESWNFDIPIPNKEIEVF
                     DTQDSINGDGQSYFIQGFSEKNFKKVFNLKGGIVVSKDNINEIEKYIDKFKRDSVNIN
                     KSNKNKIEEDFKKYKLEVKKDDKYIYKRNYENYVVLIIKKDEQKLYSLIWNQ"
     gene            complement(1156..2514)
                     /gene="rlmD"
                     /locus_tag="MJE33_001761"
     CDS             complement(1156..2514)
                     /gene="rlmD"
                     /locus_tag="MJE33_001761"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBY9647697.1"
                     /translation="MLSKNEMYIVDIVDIGQGGVGVGKYNGITVFVEGGLIHDKVKVR
                     INKVKKNYAVGDIVEIIEKSPFRVKRVCSDNLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGIPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKDTNAILGRENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYANLNDTDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNIHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMNPERIVYVSCNPSTLARDLDYLNAHGFKCVEAQPVDMFPHSTHVETVAKII
                     KQ"
     gene            complement(2689..4449)
                     /gene="pyk"
                     /locus_tag="MJE33_001762"
     CDS             complement(2689..4449)
                     /gene="pyk"
                     /locus_tag="MJE33_001762"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBY9647698.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEVLTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIVCVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRIIKNGNACVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSAADITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            complement(4501..5460)
                     /gene="pfkA"
                     /locus_tag="MJE33_001763"
     CDS             complement(4501..5460)
                     /gene="pfkA"
                     /locus_tag="MJE33_001763"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBY9647699.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            complement(5669..9238)
                     /locus_tag="MJE33_001764"
     CDS             complement(5669..9238)
                     /locus_tag="MJE33_001764"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBY9647700.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMSSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     LDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSNEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKVAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRVIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKALEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFIE
                     LEDLYGAIEIIVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFADRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNNIVEIAKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGALTCICDEFINNLEELLPKDNIKVKA"
     gene            complement(9395..10342)
                     /gene="whiA"
                     /locus_tag="MJE33_001765"
     CDS             complement(9395..10342)
                     /gene="whiA"
                     /locus_tag="MJE33_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBY9647701.1"
                     /translation="MSFSTETKNELARVISGNECCNIAELSALVKSGGSIQIVGYKKL
                     NLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEMLLK
                     KLGILEDKEGFFTINKISENLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVTNNE
                     EFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNTKIV
                     KEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIRIEY
                     EDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKS"
     gene            complement(10359..10784)
                     /locus_tag="MJE33_001766"
     CDS             complement(10359..10784)
                     /locus_tag="MJE33_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBY9647702.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            complement(10853..11959)
                     /locus_tag="MJE33_001767"
     CDS             complement(10853..11959)
                     /locus_tag="MJE33_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBY9647703.1"
                     /translation="MAKLLIIVGILGWIALIVSLFIYKKAKNEKVKLFQRSIVENREP
                     NVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIRNCLL
                     ALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFAITGR
                     VLPVTLEDVNLVAKLENGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDVIASI
                     YDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYNVLEH
                     VNAIVKHTDENLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGIKTVE
                     KNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            complement(11971..12828)
                     /gene="rapZ"
                     /locus_tag="MJE33_001768"
     CDS             complement(11971..12828)
                     /gene="rapZ"
                     /locus_tag="MJE33_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBY9647704.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIEEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            complement(12859..13572)
                     /locus_tag="MJE33_001769"
     CDS             complement(12859..13572)
                     /locus_tag="MJE33_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBY9647705.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYTDINILLGMECNILDDKGNIDMDDKFIRDFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYIFKTEKAKEYNTKAVINAISNNDIFIITHPGDKGDV
                     YIEEVAKTAKKTNTRLEINSSHSFLNVNQLNKIKGLENKFIIGSDAHCPERVGDFELA
                     MKTISDSNLDISLVENIII"
     gene            complement(13576..14490)
                     /gene="murB"
                     /locus_tag="MJE33_001770"
     CDS             complement(13576..14490)
                     /gene="murB"
                     /locus_tag="MJE33_001770"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBY9647706.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKEESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRLMDM
                     EGNIFELSNEQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            complement(14763..15533)
                     /locus_tag="MJE33_001771"
     CDS             complement(14763..15533)
                     /locus_tag="MJE33_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBY9647707.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKIDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMATASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSIVVGGIYYLCYVKNS"
     gene            15851..17332
                     /gene="cls"
                     /locus_tag="MJE33_001772"
     CDS             15851..17332
                     /gene="cls"
                     /locus_tag="MJE33_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBY9647708.1"
                     /translation="MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTW
                     LLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSD
                     IHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
                     DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKI
                     VNFNLNYRNHRKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLN
                     MRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLK
                     MIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAG
                     ELLKFGAKIYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKA
                     LEQRIIFENDILNSKEITLDIYNSRSTYIKIKESISRLLSAVL"
     gene            17650..18147
                     /locus_tag="MJE33_001773"
     CDS             17650..18147
                     /locus_tag="MJE33_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HBY9647709.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLATKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
     gene            18164..20050
                     /locus_tag="MJE33_001774"
     CDS             18164..20050
                     /locus_tag="MJE33_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HBY9647710.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            20073..21854
                     /locus_tag="MJE33_001775"
     CDS             20073..21854
                     /locus_tag="MJE33_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBY9647711.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRVCMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCVYGECPQECACEEAK"
     gene            complement(21994..23790)
                     /locus_tag="MJE33_001776"
     CDS             complement(21994..23790)
                     /locus_tag="MJE33_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HBY9647712.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGLAI
                     GFYNKNIVGIFVGVLSIIIFIALLVIHSKVKEEETYFKSKSEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENLQAKDLDLFGRASLYQYICVANTSYGKNFLAKYLWNENPDENVILE
                     RQRAIKELLSKDDFSIHIQTLSNIIGKEQKNNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTATILSFIFCMLGFLPVLPVFLLFIIQLGFSGFGYPRLMQTLAPLFSFSRSIQ
                     VYEKMFEVLEKETFESAYLKELQDKLSKGSGVSRGVKQLNSIGNAVNLRYNQILYIIA
                     CGVLMWNYHCAEALERWKGIYGNQIRDWFESIGEFEALISLTVISHVKENTCFPVIKY
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSKPWVISMVTTHDFELCDLSGSGDVEVVNYHFSEY
                     YVDDKIHFDYKIKDGRCKTTNAKQLMRMAGIL"
     gene            complement(24004..24933)
                     /locus_tag="MJE33_001777"
     CDS             complement(24004..24933)
                     /locus_tag="MJE33_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBY9647713.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
     gene            complement(24933..26750)
                     /gene="uvrC"
                     /locus_tag="MJE33_001778"
     CDS             complement(24933..26750)
                     /gene="uvrC"
                     /locus_tag="MJE33_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBY9647714.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMIEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSSILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILN
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKALTKSILDDIQGIGEKRKKSLLN
                     HFKDVDAIKKATMEELLEVDGMNKSIADNVYNFFRKEEN"
     gene            complement(27009..29834)
                     /gene="uvrA"
                     /locus_tag="MJE33_001779"
     CDS             complement(27009..29834)
                     /gene="uvrA"
                     /locus_tag="MJE33_001779"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBY9647715.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIILNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAMFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLITTLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENENSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFASTNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPKEVSKVDGSYTGQFLKKYFS"
     gene            complement(29853..31823)
                     /gene="uvrB"
                     /locus_tag="MJE33_001780"
     CDS             complement(29853..31823)
                     /gene="uvrB"
                     /locus_tag="MJE33_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBY9647716.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDTYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIVEIENELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     IDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNIIPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMMEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            complement(32010..33272)
                     /locus_tag="MJE33_001781"
     CDS             complement(32010..33272)
                     /locus_tag="MJE33_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HBY9647717.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKNLPNFKSILENCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKAKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNRHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNDEVDKALKMNDKWLG
                     DIIQATKDAGTYKDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKDFDAYV
                     NSSGISAQIIVNNLDRLTELRELLEEFKEELFIENIFTKEEASNLGLEGDFEIVIEGL
                     EGISFGNDFEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKGGVVIEEGD
                     LRAHAATILKMFNLKLDGVEKEAFDFIM"
     gene            complement(33335..34639)
                     /locus_tag="MJE33_001782"
     CDS             complement(33335..34639)
                     /locus_tag="MJE33_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBY9647718.1"
                     /translation="MKLKRKLATGLAITLLLGSLVGCSNSNDANASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQERFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INKDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            complement(34670..35530)
                     /locus_tag="MJE33_001783"
     CDS             complement(34670..35530)
                     /locus_tag="MJE33_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBY9647719.1"
                     /translation="MDIIQPIKKDKVDIKKIASKSIEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLIVQFLVMVP
                     AAYAFSKIKFKGEKMLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYVPLIIPFMTS
                     SFGIFLLRQNFMQISDEIIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            complement(35574..36443)
                     /locus_tag="MJE33_001784"
     CDS             complement(35574..36443)
                     /locus_tag="MJE33_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBY9647720.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     IWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            complement(36427..37497)
                     /locus_tag="MJE33_001785"
     CDS             complement(36427..37497)
                     /locus_tag="MJE33_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9647721.1"
                     /translation="MADIRLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGRLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLNVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGSPCMNILDIKNDIKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVVTKEVLGNETIYCLSVLNREIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDVKVCKSMYSKIKGDIYEKAI"
BASE COUNT        11825 a         6695 c         4678 g        14742 t
ORIGIN      
        1 actagaaaaa atcaaaagaa atattagaag aaatggatat tgagtaatca aagaaaagat
       61 tttgatatta caaatgatat gtttcaagaa agcattccta tcacaacaaa aattattctt
      121 gaagacatgc cttcaaatga tgaattaaat catgtttttt caaaaggttg tgaacgaaaa
      181 atgaaaaaga gaaaaattgt attaatcaca ctactattaa taggtgtttt gttattagga
      241 agtatcctat ataatctttt tttaggaaaa acagctaata tttcaatgct aaaggaatct
      301 tggaattttg atataccaat acctaacaaa gaaattgaag tatttgatac tcaagatagt
      361 attaatggag atggacaaag ttattttatt caaggatttt ctgaaaaaaa tttcaaaaaa
      421 gtttttaatt taaaaggtgg tatagttgta agcaaagata atattaatga aatagaaaaa
      481 tatattgata aatttaaaag agattctgtt aacattaata aaagtaataa aaataaaatt
      541 gaagaagatt ttaaaaaata taagttagaa gttaaaaaag atgataagta tatatataag
      601 agaaattatg agaactacgt agttttaatt ataaaaaaag atgaacaaaa attatacagt
      661 ttaatatgga atcagtaggt acatatgtat tattaagaat tataaataat aaaaataaat
      721 tttagtcaat atacaaaaac cttttaaatt gtctggaaat cacctcttca tttgttataa
      781 aaacatacaa caaatgaaga ggtgatttta tggttttaaa aaaaataatt ataagtggat
      841 atgtgctttc acatgtttat caacatttgt aatgcctata tttgctaatg aattataata
      901 taaaaatatt aaataatatt caactataga ttatgtaaat tactctatga aaaaaataag
      961 ttgtataaaa tctggctact gtagattgac gatttctttt aaaattacaa ttataataag
     1021 ttttatagaa ttaaaaataa aactttagag atttttgata acattgtaga ctaaatttgc
     1081 caccaaaatc gctttacagt ggcaaatcgg tggcaaatac aatatattat tagtatataa
     1141 tctatataaa cattactatt gtttgattat cttagctaca gtttccacat gtgtagaatg
     1201 agggaacata tctacaggtt gtgcctcaac acatttaaat ccgtgagcat ttagataatc
     1261 taaatccctt gctaaagtag aaggattaca agaaacatag acaattcttt ccggattcat
     1321 tgaaacaata gtctctaata ccttttcgtc acatcccttt ctaggtgggt ctacaactac
     1381 tacattggca gtcttaccct ctctatacat cttaggtaca atttcctctg cctttccaac
     1441 ataaaattca gcattatgta tattgtttaa tttagcattt attttagcat cttttatggc
     1501 atcatctata atttctattc cataaacctt tttagccttt tgtgccaaaa ataaagatat
     1561 tgtacctatc ccacagtaaa tatcaaaaac agtatcagta tcatttaaat ttgcatattc
     1621 aagagcctta tcatacaata ctttagtttg gacagggttt acttggaaaa atgataatgg
     1681 tgaaatctca aacactaagt cacctatata atcgtttatt tttccatctc catatataac
     1741 tatattttct cttcctagaa tagcattcgt atctttttga tttacattta caaccaaagt
     1801 cttaaatcca ggtatatttt ccttaagcac tgaagctaat tctttaagat atggaagttt
     1861 gtttccattt gctactaata caaccattac ttctttagtt gtaaaaccta tttttgttac
     1921 taaatgccta atcaaccccg tgtgagtctt ttcatcatat atacttacct tataggccct
     1981 tatatatgtt tttattattt taacaatttt atcatttatc tcatgttgta ttacgcattt
     2041 ttctgtcgat attacatcat gagtcttctt tttatagaag ccaataattg gaataccatt
     2101 attcttttgt attggaaatt gtgctttatt tctatatcta tatggattat ccattcctat
     2161 agtgtcatga acttctacat cctctaactt acctattctc gaaagtactt gtttaacttc
     2221 atttgttttc atttcaagtt gtttattata atctaagtct tgtatctgac accctccaca
     2281 atccctaaga ttgtcactac acactctttt caccctgaat ggagactttt ctattatctc
     2341 aacaatatct cccacagcat aatttttctt tactttatta atcctaactt taactttatc
     2401 atgtattaat ccgccctcta caaaaactgt aataccattg tactttccta ctcctactcc
     2461 gccttgacca atgtctacta tatctacaat atacatctcg tttttcgaaa gcataaatca
     2521 ccctccaatc aatctattat atttaaatct ttacaaactt ttttctttaa atttatatta
     2581 aaatattgct attaattaaa ctatattttt tactaagcta caatatttta ttttaccaaa
     2641 aaaaaaggag cttataaagc cccttagtaa ataaatttat tatttttctt ataaaactct
     2701 agttgaacct ctatatacca cgcctcttga tgcatccaca gttacaactt ctccatcttt
     2761 tactagattt gttatatcag ctgcagacac aataactggt atatctaaat ttatacctac
     2821 aatagctgta tgtgatgtca ttccaccatt ctcagttact acagctcctg ctttttctat
     2881 ataattgttc atttcagcat cagtagcagt agaaacaagt atgtcgcctt cattaaagtc
     2941 tacacaagca tttccattct ttattatacg aactttacct tctaccgttt gagtacctac
     3001 acctatacct tgcacgattt cttcgcttat tacatgaacc tttattaaat tagtagttcc
     3061 acttactcct actggtactc cagcagttat aactactaat tccccattat ctatataatc
     3121 agcttgtcta gctgcctcaa ttgccttttc tataacttca tctgtatttc ctgcaacaga
     3181 agacttaact ggatatactc cccatgtaag agctaattta ttcatagtct tttcattatt
     3241 tgtagtagct attattggag attttggtct aaatttagat accattctag cagtatatcc
     3301 tgaagaagta gatgttatta tagctgatgc atttaaatct actgctgttg tacaagttgc
     3361 atggcttatt gcatctgtta cagtaacatt gttagtacca ttttccttta gtaatctatc
     3421 ataatctaaa gtctcttctg ttctttttgc tattgtagcc atcattttta ctgcctctac
     3481 aggatatttt ccagcagcag tttctccaga taacattatc gcatctgttc catcatatat
     3541 agcattggca acgtctgtaa cttctgctct tgttggtctt ggatttctta tcatagaatc
     3601 aagcatttga gtggcagtaa caactggctt tgctaattca ttacattttt ttatcatcat
     3661 tttttgaact attggcatct cttcagttgg tatctcaaca cctaagtcac ctctagccac
     3721 cattatacca tcagaaactt ttaatatttc atctaggttc tctacacctt cttggttttc
     3781 tattttagat attatttgta tatcagttgc attattgttc tctaatactt ctcttattgc
     3841 tagtacatca gatgcctttc ttacaaaaga agcagctata taatctattc cttgacttat
     3901 tccaaactct atatcactta tatctttagg agttattgct ggtaaattta tcttaacacc
     3961 aggtacattt actcctttat gatttttaac tattcctgaa ttttcaacaa cacaaactat
     4021 atcttctcca tttatttctt ttactcttaa gccaactaaa ccatcatcta ttagtataga
     4081 atctcctgat ttaacgtctt cagcaagacc tttatagctt actgtacaca tttctttagt
     4141 ccccataata tctttcatag ttatagtgaa cttttgccct tcttctaaaa atacctctgg
     4201 gtcttcaaag ttacctgttc taatttctgg acctttagta tctaataata tagcaactgg
     4261 cttatttaat ttttctctaa ctttctttgc catatcaatt ctttctttat gttcttcatg
     4321 cgaaccgtga gaaaagttaa atctacacac atttagacca ttttgcataa gttgagttaa
     4381 aacttcctca ctttgtgatg ctggacctag tgtacaaaca atttttgtct tctttacatt
     4441 atttaacata tttgctcctc ctgagatatt attccacaga tttattttta atactaaaac
     4501 ttatatagat aatatttttg ccatctcata agcttcttta tcaaatactt tcttttgagc
     4561 taaagcttca cttatttcta aatcaattat tttgttttct tttattccta caactctagc
     4621 tgattttcca tctaataata attctactgc tcttactcca agtctacttg ctagtattct
     4681 atcagataca gttgggcttc ctcctctttg gacatgcccc aatacagtga ctcttaaatc
     4741 tgctcctgac tcttttttaa gttccttttc taaatcacta gcacttccga ctccttctgc
     4801 taaaacgatt atactatgtc tcttacctct tttttgagta gtctttaatc ttagagcaac
     4861 ttcatctact gtaatttcta cttctggtac tattatagtt tccgctcctc ctgcaagacc
     4921 tgcatataag gctaaatctc cacagtgtct tcccataact tctactatgt taactctttc
     4981 atgagatgat gatgtatctc ttatcttacc tatagcatct ataatagtat tcattgcagt
     5041 atcaaatcct atagtataat ctgtatatgc taaatcgtta tctatagttc ctggtatgcc
     5101 tatagctggg aatcctaagt cactaagttt ttgggctcct gcaaatgaac catctccacc
     5161 aattacaact aagcaatcaa ttttatactt tttaagaatt tttactgctt ttaatctacc
     5221 ttcttctgtt ttaaattctt cacatctgga agattttaat atagttcctc ccctgtgtat
     5281 aatatctcca acagaagata agttcatttc agttaaatct tcttctagta atcccttata
     5341 tcctctattg attccatata ctttgcatcc ataatatatt gctgatctta caaccgctct
     5401 aatcgcagca ttcattcctg gagcatctcc tccacttgtt agtaatccta tagttttcat
     5461 tttttacctc cttggatttt atgtcaattt tttattataa tcattgttat tgtattctaa
     5521 tggacttttt ttgtccctat atcacttttt aatcccatca agactaaaaa aattaaatgt
     5581 ccttaaatgt ccattttgtg atattatatc acaaaacgga caatattttt catattatta
     5641 taatatattt agatttttat ataaaaaatc aagcttttac ttttatgtta tctttaggaa
     5701 gtaactcttc taagttattt ataaattcat cacatataca agtcaatgcc ccggtcattt
     5761 tatatgcttt acgtttacca tttatatttt catccatagg gtacaagtat atactgtcat
     5821 tacctggata ttgtttggct atttctacaa tattattagt tgtatcagta tcagacatac
     5881 tatctatttt caaatacaat tttttaccac tttctggctt aatattttct acatttctag
     5941 ttcttcctga attgtagttt ctatctgcaa agtcagtatc atcatttata tcttttatct
     6001 ctctagctat aagttttgca ttttcgcctt cttttatact taatgttcct tttacatata
     6061 ttatattatc ttcattcaaa atgacattat acttttgtaa taattgagga aaaactatta
     6121 tttctatagc accatacaaa tcttctaatt caataaatgc cataatttca tttcgttttg
     6181 ttgttttaat agttttagtt acaatcattc cacccattat aacatctgtt tcattcattt
     6241 gtaggtatga ctcttcttcc tcttttaatg aatttaattt tccattatcc atagatgttt
     6301 tttcttgtag ttctttttca aattgtgata aaggatgtcc acttacatac attcccaaga
     6361 cttctttttc aaggttaagt ctctctcttt cttcgtactc ttgcctttta ggcaaactgt
     6421 atatttgttg aaaatcacta tcttcctcac tatctccaaa agcatcaaat aaagaaattt
     6481 gaccttgaac atttttcttt ctatccatag atacactttc taataaactt tcataaccag
     6541 ccatcagact agctctattt tcacttatat tatcaaaagc tccacatttt attagacttt
     6601 ctattactct cttatttgta tctttttggt ctaatctttt gactaaatct actaaatcta
     6661 caaataatcc attagattct ctttcagcga ttatattgtt tattatattt acgccaacat
     6721 tttttacggc tgctagacca aatcttatat tgtcgccttc aactgaaaac tttgaaaaac
     6781 ttttgtttat atctggaggt aatacatcta tttctagtgc tttacattct cttatatact
     6841 caactacctt atcagtattt cccattacac tggttatcaa tgctgccata aattcaactg
     6901 gataatatgt ttttagataa gctgtctcat atgctaaaac cccatatgca gctgcatgtg
     6961 atttattaaa tgcatatcta gcgaagtcta tcatatcatc aaatatttta ttagctattt
     7021 cctcttctac accatttctg atacaccctg caatttctat attaccatct tcatcaaatt
     7081 taccatgaat aaaatattgt ctttcttctt ccatcacgtc catctttttc ttactcatgg
     7141 ctcttctaac taggtcactt cgtccgtaac tataaccacc taaatctcta acgacctgca
     7201 taacctgctc ttgatatacc aaacaaccat atgtaacttc cattataggc tttaattttt
     7261 catgtagata tgttacatct tttgggttgc ttttattttt tatatatgca ggtatagaat
     7321 ccattggacc tggtctaaat agggatattc ccgctacaat atcttcaaaa ttgtctggtt
     7381 ttagctgttt cataaaactt ctcatgccag cactctccaa ttgaaacacc cctagtgtat
     7441 ttcctgatga aagcatttca tatactttag ggtcgtcata atccatcttt gaaaagtcta
     7501 ttttttctgt gtagtttttt ataactctat ttttttctat taaatcaaga gtatctctta
     7561 taactgtaag agttcttagc ccaagaaaat ccatctttaa aagcccaagt tcttcaagtg
     7621 ttgtcatagt aaactgtgtt gtgattgcat cttgatgttt gtacaaaggt acatattcat
     7681 ctacgggatt ttttgatata acaactcctg ctgcatgagt agaagcatgt cttagcattc
     7741 cctctatctg ttttgatata tttattattt cttttgtttc cgggtcttgg tcatacaatt
     7801 ctttaagatt aggatttgta tctagggcct tatctattgt cattcctaaa gcaaatggta
     7861 tttccttggc taccttgtca actttattgt agcctatatc caataccctt ccaacatctc
     7921 ttattgcagc ttttgcaccc attgttccaa aagttattat ctgggctaca tggtcatcac
     7981 catattttct ctttacataa tctataactt cttctcttct ctcataacaa aaatctatat
     8041 ctatatctgg catggatata cgttctggat ttaaaaaacg ctcaaataat agagagtact
     8101 ttattgggtc tatatcagtt attttaagtg tatatgctac tatcgaacca gccgcactac
     8161 ctctacctgg tcctactata atattatttt ctttagaaaa atttataaaa tcccaaacaa
     8221 ttaaaaaata ttctacataa cccatttttt caattacatc taattcataa tttaatctct
     8281 ctagtatctc attgctagga ttatcatatc tctcttttaa tccattaaaa cacaactccc
     8341 ttaaataagt ttctggtgta tatccttctg gaacatcata cttaggaaga tgtatagtgt
     8401 taaaatcaaa ctccacatta cacatatcag ctattttcac agtattatca atagcctcta
     8461 atgcatatgg aaataattct tccatttctt ctcttgattt taaataaaac tcatcactac
     8521 caaatctcat tctatttggg tcatttaatg tttttcccat ttgaatacac ataagtacat
     8581 catgtatttt cgaatcttct ttatttacat agtgaacatc attagtagca actagtggta
     8641 taccagtctc ttttgataat tttactaaac cagagtttac ttctttttgc tcaggtaaat
     8701 tgtgatcttg aatctccaaa aagaaatttt cttctccaaa tatgtctcta tagtcaagag
     8761 caaattgttt tgctttttca taatttctat tcattaaagc acgtgctaca tctcctgcaa
     8821 ggcatgcaga aagtgctata atccctttgt tatgcttttt aagttcttcc atgtcaactc
     8881 taggcttata gtaaaaacct tctacgtatg aagttgaaac tatttttatt aaatttttat
     8941 atccttccat attcttagct aacagaacta aatgtccttg atatttgtca tagtttgggt
     9001 ctttatctct caatccacgt gcagctgtat agacttcgca ccctataatt ggctttattc
     9061 cttcttttat agctgttttg tagaaatcta taacaccaaa catacaacca tggtcagtta
     9121 tagctattga gctcatgttt agttccttag ccctttttat aagttttttc accctcgaaa
     9181 acccatcaag taaactatac tcagtatgta catgaagatg agcaaaatct ttgttcatta
     9241 aatcacctcc tgctttataa ttttaatttt accacaaata tatgtactaa aacaaaaaca
     9301 gagactttta tctcaaaata ggaattaact tcaacgtata ccattatatg ttataaatta
     9361 atgcttattc tgagattgtc ccctcaattt attgctatga tttttttttc aaatctgttg
     9421 caatttcttc tatttttcta agtctatgat tgactccaga ttttcctatt ggtggttcta
     9481 acatctctcc aagttctttg agagtcatat cctcatactc gattctaatc ttagctatct
     9541 cttgtaaatt ttctggcaaa ctgctaattc ctatagtctc ttgtatgagc ttaatatttt
     9601 ctacttgtct tactgctgca tttactgttt tagaaaggtt agctgtctca caatttacta
     9661 tcctgtttac attatttctc atttctttta ctattttagt attttgaaga cttaaaagag
     9721 cgtggtgtcc tcctataata ctcaacaaat ctgatatttg ttcactctct ttgatataaa
     9781 caacgtgatt attttttcta gccacaattt tactattaaa acctaaagag tttataagtt
     9841 cttttagaga atctgcaaac tcctcattat tagttacaaa ttccatatga tagttctttt
     9901 ctgggtcact tatagaacct ccaccaagaa aagctcctct tatgaaagct ctcttacatc
     9961 cttcatgctt aattaagttc tctgaaattt tgtttatagt aaaaaatcct tctttatctt
    10021 caagtatacc taactttttt aataacattt ctgaacccat ttcgcttgtt accattaata
    10081 cataactgtt atttctcttt agcatttgat tcttatttac tgagattgta gtattgatat
    10141 taaaattctt ttttaataat ttaaatactt tacgggctat tgaatttaac tctgttgtta
    10201 tctttagatt tagcttctta tatccaacaa tttgtatact tccccctgat ttaactagag
    10261 ctgacaattc agctatatta caacattcat tcccagatat aactcttgct agttcatttt
    10321 tggtttcagt tgaaaaagac atatctatca cctattaact aatttttctt taaccttttt
    10381 tttatttctt gtaaggcaac ctttattatc tccttttcat catcatacct agctaagtct
    10441 actactctat ctaggtggat aaatttagca tcaataaagc cttcctcttt ttgtggaata
    10501 gtatccatat tctttgcttg catcaaatac caaaaaactg ttttatgaac tgctctattt
    10561 tcatcccagt tttctttgaa tgtatagtgt atttcaccta aatatttcaa tatatctgct
    10621 tttactccag tttcttcact tacttcacgt aatgctgcct cttggttatt ttccccttct
    10681 tctacttttc ccttgggtaa cacccaatct ccattgaact tccttagtaa aagaatcgta
    10741 ttgccaaaca ggactacacc accagcacta acctcttctc tcatcgatgt tcactcctta
    10801 aaactaacat aatgataagg atttctacac aattttttaa aatccttttt tactaacttt
    10861 tgttgtaatt atgatttagg gctaactcaa taactatgtt agatatatac tttgcatcat
    10921 gtcttatata attatttttt atttcaatta aattcttctc tacagttttt atacccatct
    10981 cttttaattt atctttttgc tttttatcta acaagacttg ctctgctcca tcttgtttat
    11041 ataagtcaag catttcttct ggcaatatct cattatttgc gattacataa tctattaaat
    11101 tctcatcagt gtgttttact atagcattaa catgctctaa tacattatac ccttctgtct
    11161 cacctggttg agtcattata tttgctatat atacttttgg agctacagac gatttaatag
    11221 cgtctacaat cccctctaca agtaaattgg gtattatact tgtatataaa cttccaggac
    11281 ccataattat aatgtcagca tcataaatag atgcaataac atcctttaat ggttttacat
    11341 cttttggatt taaaaaaatt ttatcaatag agcttttttg aatttttgac tcttcaggta
    11401 tactagattc tccatttatt atattgccat tttctaattt ggcaactaaa tttacatctt
    11461 ccaaagttac tggtaaaact cttcctgtta tagcaaaaat ttcacttaat ttatatacag
    11521 ctttttcaaa gtttccatac aacccattca tagcagccaa aaacaaattt ccaaaactct
    11581 gaccttttaa aagcccttct gtaaacctat attgcataac ttcattcatt gttggctcta
    11641 tatttgcaag tgctaataaa cagtttctta tgtctcctgg tgggagcatt cctaagtctt
    11701 ctctcaatac acctgaacct ccaccatcat cagcaacagt tacaattgca gttatatttt
    11761 gcgtagtgtg tttaagacct cttaaaaata ccgattgacc agtacctcca cctatcacaa
    11821 ccacatttgg ttctctattt tcaacaatag acctttgaaa tagttttact ttttcatttt
    11881 ttgctttttt atatataaat aacgatacaa ttaatgcaat ccaacctaaa attcctacaa
    11941 taataagtaa tttagccatg tttgcctcca ttatcgcaac atagagtctc tatgtttctt
    12001 tacaactcta tatcccttat tagataaatc ctccgctata agatttgtta tagtaactga
    12061 cctatgtctt ccacctgtgc atccaacacc aattactaga tgttgttttc cttcctctat
    12121 atattgaggt actaaaaaat gaatcatatc taaaagtttc acataaaatt cttcactgat
    12181 tttagaattc ataacataat ctctaacttc tttatcatca ccagttttag ctcttaactc
    12241 ttcaacataa tagggatttg gaagaaatct tacatcaaat actaaatctg catcagcaag
    12301 tataccatgt ttaaatccaa aagatactac agaaatagtt aggtttggat tatcttcacc
    12361 agaagaatat attttcttta tttcttcctt aaggtcttta ggtttcatgt tagttgtatc
    12421 aattatacat gtagataact cctttaaagg ttccattatc tttctttcca tttttatacc
    12481 ttctggaatc tgcatatctt ttgccaatgg atgatttctt cttgtcattt tatatctctt
    12541 taacaataca tcatcattac aatccaaata taccatttca tattcataat tttctttttc
    12601 taaataattt aagctttcat gaagcgcttc aaaaaacttt ctccctctta tatctatccc
    12661 tagagcaact ttatcaatac ttgaatttgg ttgataacat aattctgcaa atttagttat
    12721 caaagcagga ggtagattat ctacacaata aaatcccata tcctctaaag ctctcattgt
    12781 ctcacttttt ccagatcctg aaagacctgt aactataaca aatttcattg ctaactcctc
    12841 ccttttatct tatgtttatt atattataat gttttctact aacgatatat ccagattaga
    12901 gtcagatata gtcttcatag ctagttcaaa atcaccaact ctttctggac aatgtgcatc
    12961 tgagcctatt ataaatttat tttctagccc ttttatttta tttaattgat ttacattcaa
    13021 aaaagaatgg ctactattta tctcaagtct agtatttgtc ttttttgctg tctttgctac
    13081 ttcttctata tacacatcgc ctttatctcc tggatgagta attataaaaa tatcattatt
    13141 acttatcgca tttattacag cttttgtatt atattctttt gctttttcag ttttaaaaat
    13201 atagttatta caatgattta ataagcttct taatgatgtt ggagtagaac caaaatgata
    13261 tccagccata atataatcaa aatctctaat aaatttatca tccatatcta tatttccttt
    13321 atcatctaat atgttacact ccatacctaa aagtatattt atatcagtgt acttatcatt
    13381 taaataatct acttcttctc tcattttgag aatatctttt ttctttacac catatgcaat
    13441 atgtttataa ctatggtcag atattcctat tgtttttatt cctttagaaa tggctacctt
    13501 tacattatcc tcaatagtcc ctttgccatg actatataat gtatgtgtat gataatcagc
    13561 cagtattttc atttgttaat cctctccaag tattttgact tcttcttcca acataacccc
    13621 aaacttggca tatacagcac tttttataac atatattaaa tccaaaatat ctttagcact
    13681 tgcttctcct tgattgacta caaatccaca atgcttctca gatacttgtg ctcctcttaa
    13741 agtaagacct ctcaatccag tttcttctat taatttacct gcaaaatatc ctgttggtct
    13801 cttaaatgta cttcctgcac tagcaaaatt taatggttgt ttagttattc ttctcgtcgc
    13861 taaatcatcc attaaacttt ttatttcatc ataatttcct tcttgtaatt ctaattctgc
    13921 tgataaagct atatatccgt ttttcgatat tatacttttt ctatacccaa attccatctg
    13981 ttcatttgat aattcaaata tattgccttc catatccatc agtcttactg acttcactat
    14041 gtccttcatt tcaccaccat atgctccagc attcatggct aatgctcctc ccaatgtgcc
    14101 aggtattcct gatgcaaact caaacccttt taattcttgc ttttggagag ctttccctaa
    14161 aactgataat aaagctcctg actgagcagt catcctagta tcatttatct cataagaatt
    14221 gaaattatca gctagttcta ttaccactcc tcttattcca ccatctttaa ttaaaatgtt
    14281 tgaaccattt ccaattatta aatacggaat agattcttct tttaccaact taagtacatt
    14341 ttttaattgt tcttcagttc taggtctaac taatatatct gctggtcctc ctaccctaaa
    14401 tgaaatatgt tttttcatag gttcatcgac ttttatatcc tctttactta atatatttaa
    14461 taaatttata tatatatctt gattattcat tgtatttcct cccaaattat actgaacttt
    14521 agctgtcatt attatttaaa ttacattagt atatagtatt aactatattc aataatatat
    14581 gattttgctt atcatatgga ttttatattt taagcttctt aattacaaac tatattcaat
    14641 aaatatataa taaagtaaaa aaaaaaaaat aacaacatat aaaagaatat aaataattta
    14701 aaaaaagcat caatatcact gatatcaatg ctttaaaagg caatttaatt cgtattattt
    14761 atctaactat ttttgacata gcaaagataa taaatccctc caactactat ggaacctcca
    14821 acgatatttc caagtgttac aaataccaaa ttagttataa attgaaccat agttatattt
    14881 gctcctaatg ccattcccat tggtataaaa aacatgtttg caacactatg ttggaatcca
    14941 cataatacaa atagcattat tactgtccaa catgaagcca attttgacat tatatcttga
    15001 gatgctgtag ccatccatac agctaaaacg actagaatat tacaaagtat tgcttttaaa
    15061 aacattgatg ctatagttga attaacctta gcttctgcca cagctatagc ttttgtagct
    15121 gcatctcctg tcaatgtaga agaataaaac acaagtgcaa ctagcaaaat tgaacctact
    15181 aaatttgcaa aataaaccag tccccagttt cttaacattt ttcctaatgt tatttttttg
    15241 tccataagtg caatagccat taaggtgttt ccagtgaata attctgctcc tgccactact
    15301 acaagcataa gacctactgg aaagaccata gcacttaaaa agctagctac ccctgggtct
    15361 atcttgccaa aagtttgact aactatcata ctccccaatg ccccaaaacc tataaatgct
    15421 cctccaaata aacctaatag aattgcgttt tgtgtcgaaa tatttgcttt tttaatgcct
    15481 gcacttatag ttgcttgtgc agtttcccca ggtgtcaaga atcctttact cataaatcct
    15541 tcccccttaa aaattatact ttcccttgtc tttttatggc aataattata taataacaat
    15601 atgatatata ataaatttta ttaacataaa aaatatacta tatacttata tatacttgtc
    15661 tcatataatt atactattta ttatataaca actttgttaa tttcgaaata atttatattg
    15721 aatttgaaaa atttaccttt tgattattta ttttactttt taattattga ggtattatat
    15781 ataatagata tatgtttgtg ttattatata taattactta ttttaataaa tatttgaagg
    15841 agggaatttt atgtttgata tatcattttt cgaaatgtca atatatgaaa tagcagttac
    15901 tacaatatat ataattaatt tcatggttat actaaacctt atctttagag aaaaaagaaa
    15961 cataaataca actttaacat ggcttcttat attagtattg attcctgctc ttggattcat
    16021 actttatata gcttttggca gaaatatttc aaaaaacaat atgtttagat tgaaagaaaa
    16081 ggatgataag ataatcaaaa gtaatatact agatactcag gttaaactcc aatctacatc
    16141 tgaaattgat tcagatatac atcaacataa ggatatgatt tatgcattgg ctaattcaaa
    16201 taatgctcat tataccaata ataacgatgt gtggatttat gctgaatcta gtcaattttt
    16261 taacagtctt cttgaagaac ttaaaaaagc taaaaaatat ataaacatac aattttacat
    16321 tttcaaagat gacaaaatcg gaactgaaat aatagacatt cttatagaca aagctaaaga
    16381 aggtgttgaa gtaagacttt tatttgatgc agttggtggt agaacactaa aaaatagtac
    16441 actttccaga cttaaagaaa gtggtgttaa agttggaagc ttcttcccat cattcttgaa
    16501 aattgtaaac tttaacttaa actatagaaa tcaccgaaaa attgttgtca ttgatggtaa
    16561 ggtaggattt gtaggtggat ataatgttgg tgatgaatat ttaggtagaa atccaaaatt
    16621 tggtctatgg agagatacac atactaagct tactggtgat tgcgtaattg acctaaatat
    16681 gagattcatc cttgattgga gatatacaac taaagaagac ttagatttag aaaaatactt
    16741 tatagaagaa gatacaaatt ctaatcatcc ttctgaaaat attgggattc agatagtatc
    16801 aagtggtcca gatatatcag aacttgatga gattaaatat ggttacctta aaatgattca
    16861 gaaagctaga aagtatatat acatacagag tccttattta atacttgact caacttttat
    16921 agatacttta aaaatagctt gtttatctgg tgttgatgtg agagtgatga ttccttctaa
    16981 acctgaccat ccctttgtat actgggcatc atattcatat gctggagaac ttttaaagtt
    17041 tggagctaaa atctatactt atggtcaaaa tgcattttta catgctaaga caatagttat
    17101 agatgactct atatgctcta taggaacagc taatatggat atcagaagtt ttgagcttaa
    17161 tttcgaagtt aatgcattta tgtactcttc aaaaaaagca ttggaacaaa gaattatatt
    17221 tgaaaatgat attttaaatt ctaaggaaat aacattagat atatataatt ctaggtctac
    17281 ttatataaaa atcaaggaat caatttcaag actattatca gcagttttat aaatctttta
    17341 ttttataaaa attttacatt ttggtttatt ttttaaagtt ttttatatgt attatttttt
    17401 ttttattgaa tttagctcat tttttttatt ttttttaact attctgaatt attttatatt
    17461 ataatagact agtatagttt ttatttttaa taggtaactt ttttgcattg tttttataaa
    17521 taaagaaatt gttgtatatt attctttctt tattgttttg gttttgttca cccacataat
    17581 gttggggtca actcatggtt aattatttta tgaaaaataa taactttaaa aagaaaaggg
    17641 ggatttgtta tgtgcgattt tatttcacac aacaagcaat tatttgacga actagatata
    17701 tttattgact ctcttgcaac aaaggaaggt gcgttaatac aagttctaca tgaagcccaa
    17761 ggtatatttg gatatcttcc aaaagaagtt caacttcatg ttgctagaaa acttggagta
    17821 gctcctgcaa aggtatatgg ggttgttaca ttctattctt attttactac agaacctgta
    17881 ggtaagtata aaatcagtgt ctgtctagga actgtatgtt ttgtaaaagg tgctgacaaa
    17941 atcttaagtg cttttgaaaa acagcttggc ataaaagttg gagaaactac tagtgatttt
    18001 aaattttctt tagaaggttt aagatgtttg ggggcatgtg gtttagctcc agttgttact
    18061 gtaaatggta aagtttatgg taaagtgaaa cctgaccaag taagcgaaat attagatact
    18121 tatagagaat tagaattgaa ttgttaagag gggtgaataa ataatgcgta aagtaaattc
    18181 atttgatgaa ttaaaagttt tagctgatga gctgaaacct aatcttagct taagaaaaaa
    18241 ttattctaag gaaaatgtat taagaagaga attacttgtt tgttgtgata ctggatgtac
    18301 atcttcaaac agtctagaaa tagttagtga attagaaaat gaaataaaga aatcaggaat
    18361 ccaagataaa gttagtgttc gtttgacagg ttgttttggt ttctgtgctc aaggtcctat
    18421 tgtaaaagtt tatcctgata acgttttcta cgtaaaagtt gagccatctg atgctgaaaa
    18481 gatagttcaa agccacttaa ttagaaatac agttgtcgag gaattattat atgaggaaga
    18541 atctttaagt aaaaaagttg aagaacaaga agaaatgtct ttctacaaaa aacagttacg
    18601 tatagcttta aaaaattgtg gtcttataga cccagatagt atagaagaat atattgccaa
    18661 tgatggttat ttagctctag gcaaatgttt aacttctctt actcctcaag aagttattgc
    18721 agaggtaaaa acttctggcc ttcgtggtag aggtggtgca ggtttcccta ctggctctaa
    18781 atgggaagct gcttcaaaaa atccagctgg tccaacagat aaaaagtttg ttgtatgtaa
    18841 tgctgatgaa ggtgacccag gtgcatttat ggatagatct gtacttgaag gtgaccctca
    18901 tagtgtacta gaagctatgg caatatgtgg atacgctata ggttctgata caggatacat
    18961 atacataaga gcagaatatc caaaatctat agaaagatta aaagtagcca tagctcaagc
    19021 tgaaaaatac ggtttacttg gcgaaaatat cttaggcact ggatttaact ttaaattaga
    19081 attaaaatat ggtgcaggtg ctttcgtttg tggtgaaggt acagcgctaa tgcattcaat
    19141 tgaaggtaga cgtggtgaac ctagaatgaa aacttatagt tcttctaaga gtggtctatg
    19201 gaaatctcct acttgcttaa ataacgttga gacttttgct aacatacctg caattatact
    19261 taagggtgga gattggtttg ccaacttagg tactgaagat tctagtggta ctaaagtctt
    19321 tgctcttggt ggaaaagtag aaaatgtagg acttgttgaa gttccaatgg gtactacttt
    19381 aagagatatt gtatttgaaa ttggtggggg aatccctgaa ggtaagaaat ttaaggctgt
    19441 tcaaacaggt ggaccatctg gtggatgtat accaactgaa catttagaca ctcctataga
    19501 ctttggttca ttaagttcta tcggttcaat gatgggctct ggtggtatgc ttgttcttga
    19561 tgaatctgac tgtatggtag atatagccaa gttcttcctt gagtttactg ttgaagaatc
    19621 ttgtggtaaa tgtacacctt gtcgtatagg tactacaaga ttattagaaa tattaactaa
    19681 aattacagaa ggtaatggta ctctacaaga tttagatgac ctagaaaacc ttgctgaaac
    19741 tatacaaact gcttcattat gtggtcttgg taaagctgct ccaaaccctg ttttaagtac
    19801 tcttcaatat tttaaagatg agtatatagc tcatgttgta gataagaaat gtcctgctgg
    19861 taaatgtcag aacttacttt cttactttat aacagatgac tgtaagggtt gtacaaaatg
    19921 ttctagagtg tgtcctgctg gttgtataac aggttctgtt aaggaacaac atacaataga
    19981 tacttctaag tgtctaaaat gtggtgcttg tatagataat tgtacattta acgctataat
    20041 caagaaataa cttatttgga ggaggaataa caatgagttt agttaattta actattaatg
    20101 gcaaacacgt ttcagcacct agtggaactt ctatcttaga tgctgcgaaa ttaataaata
    20161 taaaaataca caacctttgt catttacata tgaatgaaat agataagctt gacacttgtg
    20221 cttcttgtcg tgtttgtatg gttgagacag agagaggatt agttccagct tgtggaacag
    20281 ttataaagga aggtatgaaa gttcagacta atagtgctaa agcattaaat gctcgtagaa
    20341 ctattgttga attattgtta tcagaccatc cacaagattg ttttgtctgt gaaaaaaatg
    20401 gagattgtga attacagact atagctgctg atttaggagt aagaaaaata agatatcaag
    20461 gttctaaatc ttttgcaggt aaagatactt ctactaaatc tttagttaaa gaccattcta
    20521 agtgtatatt atgtagaaga tgtgagactg tatgtaatga catacaaaca gtaggagcat
    20581 tatctggagt taatcgtgga tttaatactt tagttagtac attctttaac tctgatatgg
    20641 ttgaaacaga gtgtacattc tgtggtcaat gtatatctgt ctgtccaact ggagcattaa
    20701 cagaagtaga taatgtacct aaactttggg atgttttaaa taaaaaagaa aagactatag
    20761 tagtgcaagt tgctcctgct gtaagagttg ctattggtga agagtttgga ttagaaccag
    20821 ggtcaatctc tacagggaaa atggtagctg ctttaaaagc acttggattt gaacatgtat
    20881 ttgatacaaa ctttggtgca gattttacta taatggaaga agctactgaa tttatagaaa
    20941 gaatccaaaa aggtgaaaat cttcctatat taacaagttg ttgtcctgca tgggtaaact
    21001 tcttagagca caattatcca gacaagttaa acttagcatc tagctgtaag tctcctcaag
    21061 gtatgtttgg ttctatagct aaaaactact atgcacctaa aatattagga atcaatcctg
    21121 aagaacttta tgttgtatct gtaatgcctt gtgtagctaa aaaatatgaa gcttcaagag
    21181 aagaattaag tgaatctgga atactagatg ttgacctttc tataactact agagagttag
    21241 ctaagatgat aaaagaagct gcaatcgatt tacctaatct tcaagaccaa gattttgata
    21301 atcctctagg aaaatctaca ggtgctgcat ctatatttgg agcaagtggt ggagttttag
    21361 aagctgccct acgtacttca tatgagaaga taactaataa gactttagat aatgtaaact
    21421 tcactaatgt tagaggtctt aaaggaatta gggaagcaag tatagatgtt gatggaacta
    21481 cagttaatgt gtgtattgta aatacactta aaaacgctag aaagattatg gataaggtca
    21541 gaagtggaga atgtaaatac catatcatag aagttatggc atgtcctggt ggatgtgttg
    21601 gaggtgctgg tcaaccttat caccatggta atactgaaat agttgataga cgtgcaaatg
    21661 cgttatatga aatagataga aataaggcta ttcgtaaatc tcatgagaat cctgatttac
    21721 aagctattta taaagacttc tttggagaac caaatagtga tgtagcccat aaataccttc
    21781 atactcatta ctttgataag agttgtgtct atggagagtg tccacaagaa tgtgcttgtg
    21841 aagaagctaa gtagtcataa caaaatataa aatttattct taaaaaagga ttgtcttaaa
    21901 atacttttac gtaatttaag acaatcctta ttaatttcac aaattaaatt atatttctaa
    21961 atagacttat tctttaatta acctatctat taattaaaga atacctgcca ttctcataag
    22021 ttgttttgca tttgtagttt tacatcttcc atcttttatc ttatagtcaa aatgaatttt
    22081 atcgtctaca tagtactctg agaaatgata gtttacaact tcaacatctc cactacctga
    22141 aaggtcacat agctcaaagt catgtgttgt caccatactt ataacccagg gttttgataa
    22201 tttctttact gcctcacttg ctccaataat tctatcagca gagttagttc ccttaaaaat
    22261 ttcatctact agaaccaaca ttggcagttc ttttaaacta tattgaatca ttgattttat
    22321 tctaagaatc tcagcataga aagttgatat tccttgactt acatcatctt gtattctcat
    22381 agaagtaaat atagccatac aggtcgcatc aaaactctta gcgcatactg gagcacctgc
    22441 atatgccagc actaagttta caccgatact tcttaaaaat gttgtctttc cagacatatt
    22501 tgaacctgtg ataatacaag tctgagaatt taattgtatc gaatttgcta tgacactttt
    22561 ttctgctatt aatgggtggt atacctcttc aactttcaat cttggagtat cttcatattt
    22621 tattactgga aaacatgtat tttcctttac atgagaaata actgttaaac ttattagagc
    22681 ttcaaattct ccaatagatt caaaccaatc tctaatttga tttccatata tacctttcca
    22741 tcgctccaaa gcttctgcac aatgatagtt ccacatcaaa acaccacagg caattatata
    22801 tagtatctga ttatacctta ggtttacagc atttcctatt gaatttaatt gtttaacacc
    22861 tcttgaaact ccacttccct tagaaagttt atcttgtaat tctttcaaat atgcactctc
    22921 aaacgtctct ttttctaaaa cctcaaacat tttttcatat acttgtatac ttctactaaa
    22981 tgaaaataat ggagcaagag tttgcataag tcttggatat ccaaaaccac taaaccctaa
    23041 ctggattata aaaagtaaaa aaacaggtaa aacaggtaaa aatcctaaca tacaaaatat
    23101 aaaagataaa attgttgcag ttggtaaacc ccaagtaaaa atatgcatcc attttggtat
    23161 atacacattc ttattttctc catactctat aaacgattct atactactat ctgaattatt
    23221 tttttgctct ttaccaatta tattactgag tgtttgaata tgaatagaaa aatcatcttt
    23281 tgataaaagt tcttttatag ctctctgtct ttctaaaatt acattctcat ctggattttc
    23341 attccacaaa tatttggcta aaaaattttt cccataagat gtattagcta cacagatata
    23401 ttgatacaat gatgctcttc caaataagtc taaatctttt gcttgcaagt tttcatcttt
    23461 taagtattct tttccatcta ttttaaattc cttccaacca tctccaaatc tcttaatata
    23521 cttatttaat acttcgctct tgcttttaaa atatgtttct tcttctttta ctttactatg
    23581 aataactaag agtgctataa atattattat tgataaaact ccaacaaaaa tacctactat
    23641 atttttattg taaaatccaa tagcaagccc tgctatcact atcaaaaaag atattaatct
    23701 tgctgtactt atattatttg actttttact taagagcaat tctttttcag tactactttc
    23761 taaaagttta ctaaaatatt ctattcccat aaaaaattcc aactttcttg ttatataatg
    23821 aaactcaaat ataaaattaa tctagtatta ttataatcta acactgtact ttaaattatc
    23881 cttatataat atatcttatt aataaacatt atatatatac aacttgattt taatttataa
    23941 ggtgtaggat ataatcctaa tcaatattta attaaatgat gtgtagttca ctattattca
    24001 gtactactta gtatcgatta attttgataa ttttttagta aattcttgag ctgcatcgta
    24061 tcccattcct ctttgtctaa aattcattgc tgctacttct aatatcatag cagtatttct
    24121 tccaggttta acaggtacaa ctaatttctc tacattaaca ccaagtattt cttcatattc
    24181 tttatccagt cctagtctat cataatactt agtttcatcc caagcttcta aatgaactac
    24241 taaatctatc attttagaat ttttgattgc ccctactcca tataaacttc taacatctat
    24301 tattcctatt cctcttatct caagaaaatg tcttattagt tcaggtgact gacccacaag
    24361 catactttca tctaattttc ttatttctac agcatcatct gcaactaatc tattacccct
    24421 ttgaatcagt tctaatgctg tttccgactt tcctatacta ctttcacctt ttatcagtac
    24481 acctatacca taaatatcta ctagcacccc gtgaattgtt gtgtgtggtg ctaacttatt
    24541 atccaaatag tttgacagtc tatttataag tctagtggta tttctatttg tgctcaaaag
    24601 tattctttta tattttttag ccttttcaat tacatctggc ttgatttcca agtctctaga
    24661 aattattaac gctggtattt catatgagaa aaatctatct agtacttctt ctcgaatatg
    24721 actatccatt aatccaaaaa aattatattc tgtctttcca actatttgta ttctttcata
    24781 tgcaaaataa tcaaaatatc ctgcaaactg taatcctggt ctatttatat cttgagagta
    24841 gacataataa tctacatcat caggcatgta tactactttt aacttcaact cttttattat
    24901 ctctcttatt gatactttat tgctagtttc cattaatttt cttcctttct aaaaaaatta
    24961 taaacattat ctgcaatgct tttattcatt ccatctactt ctaaaagttc ttccatagta
    25021 gcttttttta tagcatctac atctttaaag tgatttaata atgatttctt tcttttttca
    25081 cctattcctt gtatatcatc taagatagat tttgtcaaag ctttatttct tagacttcta
    25141 tgatatgtta ttgcataatt atgaacttct tcttgtatac tagcaataaa tctatataaa
    25201 ttagttgttt tatctagttc tatttctttt tctttgcata tcaatccttt agttctatgc
    25261 ttatcatctt tgtacattcc ccaaagaggt atatctacat cattgagttc tagtactttt
    25321 tttactgcac taacttgtcc ttttccacca tccaacaata tcaagtcagg taaatttcca
    25381 tgttttaatc ttctatctac aatttcagcc atagaatcat aatcattagg tccaataact
    25441 gtctttattt tatatctcct atattccttc ttatcttttt tagcattcgt atacactacc
    25501 atagaaccaa tcgaatctac accttgtata tttgatatat cataagcctc tattcttatt
    25561 ggcaactttt ctagatttaa tatttgcttt aattcttcca atgctccaat acttttttca
    25621 tattttcttt tattcatatc agaaaatttt tcaagatatt ctacagcatt tttcctaacc
    25681 atttcaacta agcttttctt ctctcccttt tgaggaactc ttattgttac tttttgcccc
    25741 tttttagatg acagccattc ttctaatata gaactatctt ctatctcatc ttctataatt
    25801 aattctttag gtatatactc ttgttcatta taaaattgtt ttacaaatga acttaatata
    25861 gaagctcttg gactatccat taccccttct aatataaaat gttccctacc tacaatctta
    25921 ccatttctta taaaaaatac ctggacacaa gcttcattat gagctcttgc cattgcaacc
    25981 acatcttggt ttaagtcact tacagtagca tctatttttt gtttctgtat catctcttca
    26041 aggctcttta tcttatccct atatacagca gcctcttcaa atctaaagtt catagaactc
    26101 tcattcattt tttcctttaa aagttccatc aatttttctt ctttaccaga taaacacatt
    26161 ataatctctt ctatcatttt tccatactct tctttagaaa catttcctgt acaaggtcct
    26221 aaacatttat ttatgtgtag atttaaacaa ggtcttgtct tattctttat tgctttatct
    26281 atatctattt tacagcttct tattggatac gtactactta taagctccaa ggtatcattc
    26341 acagcagtta tattggtata gggcccaaaa tactttgcct tatctttaag aactcttcta
    26401 acttttaaga ttcttggata atcttcattc gtagttactt ttatatatgg ataagtttta
    26461 tcatctctaa gcactacatt atactttggt ctgtatcttt ttattaaatt acattcaagt
    26521 attaatgcct ctaactctga atccgttata atatactcaa acttatatat attcttaacc
    26581 atagatttta ctttagatgt atggtttttt gaagattgaa aatactgcct taccctattt
    26641 tttagagata tggcttttcc cacatatatt atatggtcgt atttgtcttt cattaaatat
    26701 actccgggct cagaaggtaa tttttttaaa tgctcttgta tatcaaacat ctcgccctcc
    26761 aaaacattaa attttatact aaaattatac cctaaaatat gaattttaaa aagttataca
    26821 atttccagtg ctttaaacaa gttaaagccc atattccctt ctataattga tttgaaaaca
    26881 attttaacca tgaagagtaa tatggacttt actttgtatt actatttaca acttatattt
    26941 gactaattta cttataacaa aacagtttat cgtatatctg ctctaaaatt tttcacaatt
    27001 tatataactt aagaaaaata tttctttaag aattgacctg tataagaacc atcaacttta
    27061 gatacttctt ttggtgttcc agttgcaact atagttccac ccttatctcc gccttctggt
    27121 cctaagtcta ttacataatc acaagtcttt ataacatcta aattatgttc tatgactata
    27181 attgtatttc ctgtatctgc tagtctttgt aatacttgga ttagcttgtc tacatctgcc
    27241 atatgaagtc cagtagtagg ttcatctaat atatacaaag tctttcctgt tggtctttta
    27301 ctaagttcag cagctaattt tatacgttgt gcttctcctc ctgataattg agttgaaggc
    27361 tgtcctaatt taatatatga aagtcctaca tccattaaag tttctaattt tctttttata
    27421 tttggtatat tttcgaaaaa ctcaacagct tcttccacat tcatatctaa tatatcagat
    27481 atagttttat ctttgtattt tacttgcaat gtttctctat tgtatctttc acctttacaa
    27541 atttcacaag gaacatatac atctggtaag aaatgcattt ctatttttat tattccatca
    27601 cccttacaag cttcacatct accacctttt acattaaagc taaatctacc ttttttataa
    27661 cctcttgctt tagcttcatt agtagatgca aaaatatctc ttataaaatc aaatacacct
    27721 gtataagtag caggatttga acgaggtgtt cttccaatag gactttggtc tatatttata
    27781 actttatcta tactttcaat tccttttatc tccttatgct taccagctct ttgcttaagt
    27841 ctattcactt tacttgcaac acctttgtaa agtatatcgt ttattaatgt actttttccg
    27901 gaacctgaaa cacctgttat acatgtaaac ttacctaatg ggaactttac atcaatattt
    27961 ttcagattat tttctgatgc tttaactatt tctataaaac ttccatttcc ttctctagta
    28021 gtctcaggaa gcaatatttg ttttttacca cttaaatatt gacccgttat tgagttctca
    28081 ttttcaatta tttcatctaa agtaccctgt gcaactattt caccaccatg tatacctgca
    28141 cctggaccaa tgtctataac ataatcagct tctctcattg tgtcttcatc atgctctaca
    28201 acgattaagg tatttcctaa atctgttaga tgtctaagag tagttataag cctatcatta
    28261 tctctttggt gaagccctat acttggctca tcaagtacat ataaaactcc aaccaatgca
    28321 gaacctattt gagtagctaa tcttatacgt tgcgcttctc ctcctgataa agttccagct
    28381 tttcttgata aatttaaata atcaagacct acatctctta agaacatagc tcttcctttt
    28441 atctctttta taatctcatg agctataaat ctctgttttt ctgtaagatt tatattttcc
    28501 ataaagtcaa tcaattcatt tacagataag tttgtaactt ccattatatt ttttccatca
    28561 actaaaacag ataaaacttc ctttttaagt ctcatccctt tacactttgg acatggtctc
    28621 tctgccatgt attcttcaat cttatctctc atgtaatctg aattagtttc tctatatctt
    28681 ctctctaagt ttactattac accttcaaag tatgatttat aatttctagt tcctccaaat
    28741 tttgactcat aagtaaactc tacaattata ttatctttac cataaagtaa ttcctccaca
    28801 aaatcatctg gtaaatcttt aaacggtgtt gtcaaagata cattaaaatg ctctgctaaa
    28861 ctttttatca ttttgctgta ataagtgtca tcattaacac ctgttgaacc ccatgctgcc
    28921 actgcacctt gttttattga taaatctttg tttggtacta ctaattctgg gtcaacttct
    28981 ttactttccc caagaccatt acaaacatca caagcaccaa agggagcatt aaatgaaaac
    29041 attcttggag ataattcttc tatgccaatt ccatgttctg ggcatgcaaa tttagttgaa
    29101 aaaagaattt cttctccatc tattatttgg gctattacaa gaccatcaga tagcttaact
    29161 gcagtttcaa ttgaatctgc tagtcttgat tctattccat ctttaattac tattctgtcc
    29221 actacaactt ctatattatg tttttgattt ttatttaaga ttatatcatc actaacttca
    29281 tagttttcac cattaactct aactcttaca aatccttcct ttttgatatt atcaaagact
    29341 tttttatgtg tacctttttg tcctctaaca attggtgaaa gaatttgtaa tttcgttcta
    29401 tctggaaaat ctagcatttt atcaactatt tcttgaatag tcatctgact tattttttca
    29461 ccacatgtag ggcaatacac atctcctact cttgcaaata atagtctcaa ataatcatat
    29521 atttctgtta ctgttccaac cgtagaacgt gggttttttg aggtagtctt ctggtcaatt
    29581 gagatagctg gtgataaacc ctctatatat tctacatttg gcttttccat ctgacctaaa
    29641 aattgtcttg cataagcaga tagactttct acatatcttc tctgtccttc tgcatatatt
    29701 gtatcaaatg ccaaagaaga cttcccagac cctgacaaac ctgtaaaaac aataaattta
    29761 tctcttggta attctaaatc tatatttttt aaattatgtt cttttgctcc tcttattata
    29821 attttatctt ccataatgtt gtttttacct ttctattttt ccaatttttc ttctaattgt
    29881 ttcactttat ctctaagttc tgctgctctt tcaaattgta agttttgagc agcttccatc
    29941 atctctgatt ttaatttatc tatattagct cttatttcgt ctttatcatc tgtatcagat
    30001 attccatata caacttcttc ctctgcaacc ttagtagctt ctatactatc tcttatattt
    30061 ttctgtatag ttttaggaat gatattgtgc tcttcattat ataaattttg tatatctctt
    30121 cttctctttg tttcatcaat agcattttgc attgagtcag ttatcctgtc tgcatacata
    30181 attactcttc cattttcatt tctagctgca cgtccaatag tctgtattaa agctgtttcg
    30241 gaacgtaaga aaccttcttt gtctgcatct aaaattgcta taagtgaaac ttctggtata
    30301 tccaatcctt ctctaagtaa gtttattcct acaaggacat caaacttccc taatcttaaa
    30361 tctcttatta tttctgttct ttctaatgta actatatctg agtgtaaata tttaactttt
    30421 atccctattt ctttaagata gttagtcaag tcctcactca tttttttagt taaagtggtt
    30481 ataagcactc tttcattttt ttctatagtt ttagttattt ctcctaccaa atcatctatt
    30541 tgattattta taggtcttac ttctacaatt gggtctaaaa ggccagttgg tcttattatc
    30601 tgttctgcta ttgtctctga gtgttgaatt tcataaggtc ctggtgtagc acttacaaac
    30661 aacatttgat ttatatttcc ttcaaattct tgaaaattta atggtctatt gtcaagtgca
    30721 gaaggtaatc taaaaccatt ctctataagt gatgtttttc tagacctgtc tcctgcatac
    30781 atacctctta cttgtggtat agtgacatgt gcctcatcta taattatcaa ataatcatct
    30841 gggaagaaat ccattaaagt atatggtctt tctccttctg accttcctgt aatatgtcta
    30901 gaatagttct ctatcccctg acaaaatcct atttccttta acatttctat atcatactta
    30961 gttctttgtt caattctttg tgcttctaat agcctatcat tttccttgaa aaatttaatt
    31021 tgttcttgaa gctcgttttc tatttctaca attgcctttt caattctttc tggcgttgtt
    31081 acatagtgag atgctgggaa tataacaaca tgatttctag taccaacaat cttccctgtt
    31141 acataatcaa tttctgttat tctatctacc tcatcaccaa aaaattctat tcttattgct
    31201 ttttcatcat tactagctgg gaatatttct agtatatctc ctctaactct aaaggtacct
    31261 cgtgtaaagt ttatgtcatt tctttcgtat tgtatctcta ttaacctttt tattacatca
    31321 tctctgtctc tttgcatacc tggtcttata gataacataa gttctttata gtcttttggg
    31381 tcaccaagtc catatataca agatacagat gatattatta tagtatctct tctctctaaa
    31441 atagatgctg tagcagaatg tcgaagttta tctatttcat catttatgct tgcatctttt
    31501 tctatataag tgtcactgtg agctacatat gcttctggtt gataataatc atagtaactt
    31561 acaaagtatt caactgcatt gtctgggaaa aattctttaa actcactgta aagttgtgct
    31621 gctagagttt tattatgagc cattattagg gtaggtttct taacttgttg aatgatattt
    31681 gccattgtga atgtttttcc tgaacccgtt acacctaata atgtcgaaaa tttctcattt
    31741 ctatttatag aatccactat tgatttaata gcttctggtt ggtcacctgt aggtttgaag
    31801 tctgatttta ttttaaaatc catagtatca ctcctttttt cgaacattag ttcctgacat
    31861 gtattattat atcatacctt tataaaattg tttaagttat tttaaatatt ttacccattt
    31921 aaattttact taaatctaaa acataaaaaa agacttctat ctttgcaata gaagtctata
    31981 attcatggaa aataaatact ttattcaact tacataataa aatcaaatgc ttctttttca
    32041 actccatcta attttaaatt aaacattttt aatatagttg ctgcatgtgc tctcaggtct
    32101 ccttcttcta taaccacacc acctttaata tttggtccaa aagctataaa tggaggtcta
    32161 ttacctttag taggtaaatg accatgtgtc gcaacagcaa acttgtaatc ttttatatct
    32221 gaatctttta taatacttcc ttcgaaatca tttccaaatg aaattccttc aagaccttca
    32281 atcactattt caaaatcacc ttcaagacca aggttagatg cttcctcttt ggtaaaaata
    32341 ttctctatga ataattcttc tttaaattct tcaagcagtt ctcttaattc tgttaaccta
    32401 tctaagttat ttactattat ttgagctgat attccagaag aatttacata ggcatcaaaa
    32461 tccttaactt ctccattttc aacttttata aatccatttg atttaagcaa gacatttgga
    32521 tttataactt tatcaactct aagatgacca tggtccccaa gtattataaa atttgtatct
    32581 ttgtatgtac ctgcatcctt tgtagcctga ataatatctc ctagccattt atcattcatt
    32641 tttaatgctt tatctacttc gtcattaaat aatccataat tatgtcgtgt atggtccaat
    32701 gtagctaaat gtattagcat taaatctggt ttatattcct ttattatatc tactgaacag
    32761 tttactccaa ataaatccat gttgggttct aatttccaat cgatataatg acaatgtcta
    32821 ttgtaaatac cgtccattat aaccttactg gaacttcgtt caaaagtttc tcttgggtca
    32881 tcttctctac taggagccca tatttctgca atattataat ctatatttgg atttgctccc
    32941 attacaggcc ataatacact agaggtagtt agattatttt cttttgctac atctataata
    33001 gtttttgctt ttatgtcctt tgagtaccag taccagtctt tattttcttt atttgggtca
    33061 aatttttcat tatgatatat accatgtttt ttaggatata ctcctgtaac catactagta
    33121 tgacacggat atgtcagagt gggataaaca caatttatat tttttactat ggagcaattt
    33181 tctagtatag atttaaagtt tggtagattt tttacaaatt ccaagtcttt ttcaaataaa
    33241 gaatctactg ataatactat aacctttgtc atttacatta cctcttcatt tatatattat
    33301 tgaatacttt taatcaactt acaagtctat ataattattt atttgtttta tctattactg
    33361 cttgcattgc agaagttcct ttgcttacta catcttttgg atttgcattt aaatcagtaa
    33421 gccccttttt aatctcatcc attagcttat cagttgcttc cttgtatcca tttaacattg
    33481 gaagtgcaac agcatattgt aattggtcag ttgcaacctt atattgaggt ttttctttat
    33541 ataatgtttg taacttttct gagtttgttg catcttcagt tgttggtaag tatcctgttg
    33601 atgaactaaa ctgaacgatt ttatctgttt gtgtcatcca ttctataaat tctgcacttg
    33661 cttctttttc tttatctgtt ccaccttcaa gcataactag attagctcca cctgtaggag
    33721 ttgcgtattt ttgatttttt ggtaagaatg ctgttgatat ttcaaactta ccttctgttt
    33781 gttgcataag tcctgctaaa tctgctgttg atgtaaatat catacctact ttaccattta
    33841 caaaatcttg ttttgctacg tcccaggcat tataatcttc tccaggaggc attttcatta
    33901 ttccagcttt catcatatct tgccaaaact taactggtgc ttgtcctgcc tcattattaa
    33961 atgctacttt tttaccatta gttatttctc ctccactttg cattaccata gcttcataga
    34021 accaaatatc tattggcgtt tcaaaaccat aagtatcttc tgatttattt gtcattttac
    34081 tagcataatc cttaagctct tcccatgatt ttggacctga agggtcaaga cctgctttag
    34141 ttgccatatc tttatttatg tataatattg gtgtacttct attaaatggc ataccaacaa
    34201 acttatcctc ataatatgaa tttgttaaaa gaccagaaaa gaatctttct tgatatgttt
    34261 tatcaaaata tttactcata tctactaaag cacctgctga tgcgaatgta cctgtttgag
    34321 ttatttctaa taatgttaca tctggttggt ctttagatgt taaggctgcc tgtagttttg
    34381 ttgcattttc atagtaatct ttctgtttta acatatttac ttgtacatcc ttatgttcac
    34441 tattaaattg gtcaaccatt ttttgagcag tttctccatt agttccacct aaaggagccc
    34501 agaaatctat cttcgtaata ttgctatcag tcttttctcc tttagatgca tttgcatcat
    34561 tagaatttga acacccaact aaagaaccta ataataatgt tattgctaat cctgttgcta
    34621 attttctttt aagcttcatt ttcatcctcc aaatttagtc tttaattttt tattttattc
    34681 ctgaatacat aaaagcttct tttacttttt tattcgcaaa tatataaaca agaattattg
    34741 gtaaaattag aattatattt cctgccatta ttacattcca tgcagtaatt ccttctgaag
    34801 atttaaggag cgcaactcct attggtaaag ttcttattgc atctgtattt gtcattacta
    34861 agggccagaa ataattattc caatggtata taaaattaaa taatataaat gttattactg
    34921 ctggttttgc cataggaacc attattctaa acattatttt taagtcagaa gcattatcta
    34981 gtttagctgc ttcaataatt tcatctgaaa tctgcataaa attctgtcta agcagaaata
    35041 tcccaaatga agatgtcata aatggaatta taagtggaac ataagtattt ataaatccaa
    35101 acttactcat catcatatag actggtagaa aagttacttg tggtggaatc ataagtccta
    35161 caagcaaaag cccaaatagc attttttcac ctttaaattt aatcttagaa aatgcatatg
    35221 ctgctggaac cataactaaa aattgtacaa ttaaaacact tcctgttaca aatacactgt
    35281 ttagcaaata tctaaaaaat ggacctgaat gccatgctgt actataattt tctaacacta
    35341 tatcatttgg tataatagtg ggcggtaatt gcatcatttc ttgtggattt ttaaaggatg
    35401 tagatagcat ccatataaaa gggaatataa atattgcaga taaaagtaat agaattaata
    35461 tttctattga ttttgatgct atttttttta tatcaacttt gtcttttttg ataggttgta
    35521 taatatccat tgtctcctcc ttttggataa accttataat atctactttt tctttattga
    35581 taatgaactt tctttgataa gagtctaaag ttagcatatg tagcaatcat aactatagct
    35641 aacaaaaaca tactcgctgc actcgcttgt cctatgtcat agtatataaa tccagactca
    35701 tatatataat aagataacat gttagttgaa cccactggcc caccttgtgt cattatactt
    35761 atctcatcaa atacttgaaa agatgcagtt gttgttgtta tcataagaaa aaatattgtt
    35821 ggtgataata gtggcaatgt cactttaaag aaattcgtta ttttgtttgc attatctaat
    35881 ttagatgctt catatatata gtttggtatg gatttaagac ctgataacac tattaagata
    35941 ttatatccaa gagtcttcca tattcctatt agaattagtg acattaaagc tgaactttca
    36001 cttgaaagcc actttgatgt tggtaaatgt aatgtcgata gtatccagtt taaaaagcca
    36061 tattgtggct ccataatcca aagccacaat acagctacag atactagtga aattacatgt
    36121 ggtgtaaaga ttatactttg cgttaaattg tgtattatag aatttttatt tagccaaaca
    36181 gcaagtacta tggctagtga tatagatata gtcaccataa aaaaagtata aattgcagtg
    36241 ttcgttatag tttgtataaa ctctggattc tttaaaagtt ctgtataatt gtttaagcct
    36301 acattttcaa aggttgaact aagaagacta ccagatttga agcttagaga tatcgtctta
    36361 attattggat agactataaa caataaagtt cctataatag caggtgttat atataaatat
    36421 gctttattaa attgcttttt cataaatatc tcctttaatt ttagaataca tacttttaca
    36481 tacttttaca tcttctattc tttctccatt tttatcaaaa taatataaat ctttttctaa
    36541 tacacttatt cttaaagttc ttccaatatc aaatatatca ttttccgttt tgactcttat
    36601 ctctctatta agaacagata aacaatatat agtttcattt cccaacactt ctttagttac
    36661 aacttttcca aatagactaa atacatcatc atttttccca tctatgttta tactctctag
    36721 tgttgctttt tctggtctga atccaaattt aatatcattt tttatatcta gtatgttcat
    36781 acaaggactc cctataaatt gagctacaaa taaattttta ggattttgat atatcttctt
    36841 tggagaacct atttgcatta tctttccttt gttcattata actatcttat ctgccatcga
    36901 cattgcctca acctggtcat gagttacata aacaaatgta gactttagtt tttgatgtaa
    36961 ttgtattaat tctgttctca tttgatttct taatttagca tctaaatttg acaatggttc
    37021 atccattaaa aatacctttg gatttttaac catagctcta gcaagtgcca ctctttgtct
    37081 ttgacctcca gataagttgc ctggtttcac atttaaatat tctctcaatc ctactacatc
    37141 aataacttct gttataagtt tttctctttt ttctttttct acttttttgt ttttaagacc
    37201 aaattctatg ttttctctaa cagtcatatg tggatataat gcataatttt gaaataccat
    37261 agctatatcc cttttactag catccgtttt tgtaacatca ttttcttcaa tcttcaacct
    37321 acctgaagtt atatcttcaa gacctgcaat cattcttaac gtagtagatt taccacatcc
    37381 acttgggcct accaatactg taaaagaacc atcttctata gtaagattta aattttctat
    37441 aactgtattt tttttattat aagattttgt tacattttct agtcttatat ctgccatttt
    37501 tatcctccca aaatatttaa tttaattcta cgatttactt atagattaat aatactctta
    37561 atttgtaaag gatatattaa gacaaagtga atatatagta aacttattaa aataaaggtt
    37621 aaattataaa cattttttgt taaatatact gaagttaaga tgatatacag cttatgttag
    37681 ctataatttt ctatattttt catattcatg gtttgctatc acaatgtgat tttaatttat
    37741 gaagagtcat ataaaactat ttatttttta tataatttgg tttgaaaata aaaaagacct
    37801 ctatggggga tagaggtctt taagtggggg agattgagta atttattttt gctatattaa
    37861 gttttacaat tatattatgt cccaaattta caaggtttaa acataaaaaa taaaactttt
    37921 ttaattttta taaatttacc
//
LOCUS       DAGSRM010000033        37232 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.033, whole genome shotgun sequence.
ACCESSION   DAGSRM010000033 DAGSRM010000000
VERSION     DAGSRM010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37232
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.033"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(130..324)
                     /locus_tag="MJE33_001786"
     CDS             complement(130..324)
                     /locus_tag="MJE33_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647722.1"
                     /translation="MFQVINFLCLFCVGFLVLDVMIYFCKDAMDIFEGTTNVNKVKSI
                     NVWADRVRANSYSYENKIAK"
     gene            complement(633..2162)
                     /gene="lysS"
                     /locus_tag="MJE33_001787"
     CDS             complement(633..2162)
                     /gene="lysS"
                     /locus_tag="MJE33_001787"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBY9647723.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMDIKDNFDSLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNVEDPRRTDRFEAFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNPNKEEE
                     N"
     gene            complement(2177..2656)
                     /gene="greA"
                     /locus_tag="MJE33_001788"
     CDS             complement(2177..2656)
                     /gene="greA"
                     /locus_tag="MJE33_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBY9647724.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            complement(2765..3733)
                     /gene="dusB"
                     /locus_tag="MJE33_001789"
     CDS             complement(2765..3733)
                     /gene="dusB"
                     /locus_tag="MJE33_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBY9647725.1"
                     /translation="MKIGNLELKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEEEEHPVAVQIFGSEPEFMGRAAEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYTGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            complement(3870..4640)
                     /locus_tag="MJE33_001790"
     CDS             complement(3870..4640)
                     /locus_tag="MJE33_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HBY9647726.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIISMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            complement(4659..5249)
                     /locus_tag="MJE33_001791"
     CDS             complement(4659..5249)
                     /locus_tag="MJE33_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBY9647727.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGAAIGFIFGISSLLNAITNPTVTSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYISGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            complement(5432..6400)
                     /locus_tag="MJE33_001792"
     CDS             complement(5432..6400)
                     /locus_tag="MJE33_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HBY9647728.1"
                     /translation="MYNNILDIEGLKVGQVEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMVQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGSFKCRPDLKMGYRACEVANTDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLVVSALIAVNALGDVYENGKVIAGALNDKKTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKSIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIVKKREI"
     gene            complement(6393..7370)
                     /locus_tag="MJE33_001793"
     CDS             complement(6393..7370)
                     /locus_tag="MJE33_001793"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBY9647729.1"
                     /translation="MRDKIIEIILNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNIYYKLDTEFIGKNIEHLDTIDSTNEHAKRIAIN
                     SVDGTVILSEHQDMGKGRLGRRWDSKPHEGIWMSIILKPNMEPFKAPFITLIAGASVT
                     KALNNLGANTLIKWPNDVILNGKKICGILTELSAEIERTNYVVLGIGINVKIMDFEQE
                     IADKATSLYKEGYMLSRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNRELVKCLDINEEGNLLVQRSDGSIKEIISGEVSIRGEKGYV"
     gene            complement(7685..9655)
                     /gene="ftsH"
                     /locus_tag="MJE33_001794"
     CDS             complement(7685..9655)
                     /gene="ftsH"
                     /locus_tag="MJE33_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBY9647730.1"
                     /translation="MNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLKFSEVYREL
                     TDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKAGKLTFGGE
                     AKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVHKDDEKTRV
                     TFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKTYLSRAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVGRKRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDRQVVVGTPD
                     VKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTARKREKKIK
                     METIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPVHQVTIVPR
                     GRAGGFTMQLPVEDKFYATKNEMKENIVVLLGGRVAEELVLKDVSTGASNDLERVTAT
                     ARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETKRIVDGAYD
                     KTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTEENSSKEVE
                     EQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
     gene            complement(9689..11077)
                     /gene="tilS"
                     /locus_tag="MJE33_001795"
     CDS             complement(9689..11077)
                     /gene="tilS"
                     /locus_tag="MJE33_001795"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBY9647731.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIIFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERNEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDDFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDSIILTNEEIVNEE
                     IEFYYNVPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDSEGIICVGDYKISENYKIDEN
                     TKEVLKINFNKL"
     gene            complement(11260..11688)
                     /locus_tag="MJE33_001796"
     CDS             complement(11260..11688)
                     /locus_tag="MJE33_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBY9647732.1"
                     /translation="MEEKSLSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVI
                     YKEKEEDIEITNTIKILKNQVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDT
                     RELDINIQEGEHIKLKIDYNIKVQDLFLGRNKIDIYIGTK"
     gene            complement(11705..12511)
                     /locus_tag="MJE33_001797"
     CDS             complement(11705..12511)
                     /locus_tag="MJE33_001797"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBY9647733.1"
                     /translation="MNNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKI
                     LEVQNIINNTEIHGTYQYYVSDIPEKFSDIAREFLKKKIDKIQKVEIQKY"
     gene            complement(12713..13198)
                     /locus_tag="MJE33_001798"
     CDS             complement(12713..13198)
                     /locus_tag="MJE33_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HBY9647734.1"
                     /translation="MATLLALTFIFMPIKEVFAYEPTENIKKVNKQEKNIAKKEVINL
                     SDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQKNAFSVVL
                     DGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMRIGNHVFA
                     K"
     gene            complement(13249..13908)
                     /gene="spoIIR"
                     /locus_tag="MJE33_001799"
     CDS             complement(13249..13908)
                     /gene="spoIIR"
                     /locus_tag="MJE33_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HBY9647735.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILKNGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVLEVVKDLEKK
                     F"
     gene            complement(14053..14733)
                     /locus_tag="MJE33_001800"
     CDS             complement(14053..14733)
                     /locus_tag="MJE33_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY9647736.1"
                     /translation="MENLTKLNLDNYKPLRDVVFENLREAILEGKLKPGQRLMEVQLA
                     EQLGVSRTPVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLEVRASLEGLAAYL
                     AAERISDEDIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKATNNKKLIQLINS
                     LWEQVYRFRVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYATEHIEKAEQFMID
                     KAMISKDL"
     gene            complement(14717..15607)
                     /locus_tag="MJE33_001801"
     CDS             complement(14717..15607)
                     /locus_tag="MJE33_001801"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBY9647737.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELDEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNHLWELRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTKIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTIGKHKEISDIKQVMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(15725..17275)
                     /locus_tag="MJE33_001802"
     CDS             complement(15725..17275)
                     /locus_tag="MJE33_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HBY9647738.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVVQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVEGIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDDIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNRDQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(17369..17635)
                     /locus_tag="MJE33_001803"
     CDS             complement(17369..17635)
                     /locus_tag="MJE33_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBY9647739.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(17783..18643)
                     /gene="yabG"
                     /locus_tag="MJE33_001804"
     CDS             complement(17783..18643)
                     /gene="yabG"
                     /locus_tag="MJE33_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HBY9647740.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(18727..19665)
                     /locus_tag="MJE33_001805"
     CDS             complement(18727..19665)
                     /locus_tag="MJE33_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HBY9647741.1"
                     /translation="MKFIDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMANKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLKKFYDLGVRMMTISWNHVNELSFPHNKVEYREKGLTDFGREVVH
                     KMNELGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDDIEKIYYKNALRVIKEVL"
     gene            complement(19775..19936)
                     /locus_tag="MJE33_001806"
     CDS             complement(19775..19936)
                     /locus_tag="MJE33_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647742.1"
                     /translation="MFILRFADDDDNLSVKDFTSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(19946..20443)
                     /locus_tag="MJE33_001807"
     CDS             complement(19946..20443)
                     /locus_tag="MJE33_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBY9647743.1"
                     /translation="MSLYTEWRNLSDNHESQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEEVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKAYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(20558..21268)
                     /locus_tag="MJE33_001808"
     CDS             complement(20558..21268)
                     /locus_tag="MJE33_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0489 family protein"
                     /protein_id="HBY9647744.1"
                     /translation="MENYEYSGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNDVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYVLGNKKIYVFDNHNHAFYFWAKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYDVDVNNLKDVFRYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFDLEVESEFVLDIDLDIFSSDMDYIPFELKFYKIKNLIKKAKVITIATSPYFI
                     NQEYAIKVLKELFNYDII"
     gene            complement(21444..21974)
                     /locus_tag="MJE33_001809"
     CDS             complement(21444..21974)
                     /locus_tag="MJE33_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647745.1"
                     /translation="MDIRALPIRITKEEAIEIAKSGKNSLFSKLILKNKYAHEVRLHY
                     IEFKIITLKIHKTKSIFKNRRYSNYINELKILVNGSTGSGSIVQDFPTYEVVKNVDCN
                     IVQYSDKDERRVKAKANKMAMRLTHRFMGGIPEIEIVKIESIFRPYWVVFFGKVELNR
                     RVSYLPIEADGFVIGM"
     gene            complement(21991..22221)
                     /locus_tag="MJE33_001810"
     CDS             complement(21991..22221)
                     /locus_tag="MJE33_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647746.1"
                     /translation="MSKLVSKVKKDLEIKWLGIDGFLVLLYFLITGFIFYGILTRNDI
                     IVGKIIPYGVMSVVIVIFFDYIYRVLKMTRKK"
     gene            complement(22236..23630)
                     /locus_tag="MJE33_001811"
     CDS             complement(22236..23630)
                     /locus_tag="MJE33_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HBY9647747.1"
                     /translation="MLTFWENGIIVIFAFILLGIGYYFSRNVKDMESYYLGNRSLPWS
                     LIVGTLVASWYGGVGTVGSVEYAAIYGISAWAVWSIAAHIGRMPLALWVGPRIHIRTN
                     ITVPDLLESFYGKHVAIIGAILMFIYCAQLGEITAMGTIGNVAWKIDKELIGLIMVGV
                     VVVLTVLGGLMGVAITDMVLFFCMVFGLTMVMPGQFEQIGGFQGLTEALKDAPQLMHP
                     TKGMSAMKALMLIVYSFGAYSDPTFYQRFSAADSPKVGRRALLTCFSLWICFDMVLTM
                     TGLIVRATYPEMAPAAGYITLVLDTLPQGIRVIFIIGILGSIISTLDSYYLAAGATLA
                     NDIYGRITKKELSQKQLVKFTRIGVCLAAVMGLSVAFRFENVYDACIFVGSIWMGSAF
                     VPIVGGLLGNGRRTSMGGILGMIVGGVTFGYFKIFPVQSFELEPLVIAIPFSFIAWMI
                     GNRIGENLNTQNNL"
     gene            complement(23756..25009)
                     /locus_tag="MJE33_001812"
     CDS             complement(23756..25009)
                     /locus_tag="MJE33_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY9647748.1"
                     /translation="MESKNNVKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALALS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGILGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGILLFFLVEEKKDSGASDEGLSIKSLLKVVTMPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPKSTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKGLNNKIA"
     gene            complement(25060..26214)
                     /locus_tag="MJE33_001813"
     CDS             complement(25060..26214)
                     /locus_tag="MJE33_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBY9647749.1"
                     /translation="MSNNINQDLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKEAMRPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVAGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEENVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGINGLTRDQASRRLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIEKLK"
     gene            complement(26774..27343)
                     /locus_tag="MJE33_001814"
     CDS             complement(26774..27343)
                     /locus_tag="MJE33_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBY9647750.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFAKKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSI"
     gene            27546..27773
                     /locus_tag="MJE33_001815"
     CDS             27546..27773
                     /locus_tag="MJE33_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBY9647751.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
     gene            complement(27992..28735)
                     /locus_tag="MJE33_001816"
     CDS             complement(27992..28735)
                     /locus_tag="MJE33_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBY9647752.1"
                     /translation="MENYQSTNTCTYNILYVQFNEDFERSKYFSYDVKYPVIYNIRNQ
                     STYVNPVILNNINNAVRMTVNNFKNGLQEEEREYNNATSQNSLPKQSYWVSTSYAVTF
                     NKNYVLSMILNLMGFANNYGPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDY
                     IKSQINENREMYYSNVELVIPEDQAFYLTDNGIIIYFGVDEIAPESFGIPKFELKFSD
                     FGNFINLRFYCISPDIYVQSRGRAKHFMG"
     gene            28903..29775
                     /locus_tag="MJE33_001817"
     CDS             28903..29775
                     /locus_tag="MJE33_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBY9647753.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGSYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENIEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGDIYGGELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            29775..30440
                     /locus_tag="MJE33_001818"
     CDS             29775..30440
                     /locus_tag="MJE33_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBY9647754.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTFKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKSHKDAKEAELNYNVLDFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEICHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            30461..31318
                     /locus_tag="MJE33_001819"
     CDS             30461..31318
                     /locus_tag="MJE33_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBY9647755.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIDLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSTKKETMEKGMKVDEVGKIVRKHIPRRSILSIEYEEGLSSEETMELSRRQIANILE
                     DNISVFYGGVSGLVSIDKFLKEDKVIYDNSFVTVERDLFDIKSQKHITEISSGDFICM
                     TYKGPYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(31474..34020)
                     /locus_tag="MJE33_001820"
     CDS             complement(31474..34020)
                     /locus_tag="MJE33_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBY9647756.1"
                     /translation="MNIFNKVALQGLKKNRTRTLVTIIGVVLSAAMITAVTTFGVSLM
                     NYMTNGAIIKYGDWHVQFLDVDSSFVQEQRVNDEVANTATFENIGYATLEGNRNPDKP
                     YLFIAGFSKEAFDTLPITLISGRLPENSREILVSSKVATDGGVKFAVGDTISLDVGKR
                     MSENEKLGQSDPYTAGSEVFVPQDKKTYTVVGICRKPVFEESSAPGYTLITKEDKQVK
                     TDSLSLFVTLKNPHQIRSYISSTKDHASIFNDNVLRFIGVSDNNLFNMFLYSVGGIVL
                     AIIMLGSIFLIYNSFNMSLNERTHQFGILSSVGATAKQLRNSVLFEGICIGAIGIPIG
                     VAIGIVSIGLVISVIAKNFANILYVNVPLTLTLSIPAIIVAAVVSMVTILISAYIPAR
                     KAANTPVMECIRQTNEVKIESKSVKTSKLTQRVYGLEGVLAMKNFKRNKKRYRSIVLS
                     LVLSIVLFISTSALVADLKQMSNQRSEVTDQDIIFSTQGMKDNEMLKLYDKLKTANGV
                     YEGLYQKLMTYSCVAKVSELTDDYLNYEGSHSMSEMLELPVDMQFLDDRTYLNIIKSL
                     GLPAEEYTGQNAKLIAVSMVTKDNNIIDIFNSSSMNVSLTPETNGKLEIPERQNVNIS
                     FVKVVPPDSPPGIRSSDVKKASFRVLAPYSAKDKFEIPGVSTGIESLSFQSKNPTQSV
                     AEMEQIIESEGVTNEYNLYNMYGMMDENRNYIFIANVFAYTFIIMISLISIANVFNTI
                     STNIKLRRRELAMLRSVGMSEHDFQKMMNFECAFYGMRALFFGLPIAVISSWLIHKVM
                     VTDDIDFVIPWFSIGISIFSVLFIVFITMLYTINKIKKENIIDALRDDMA"
     gene            complement(34020..34706)
                     /locus_tag="MJE33_001821"
     CDS             complement(34020..34706)
                     /locus_tag="MJE33_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9647757.1"
                     /translation="MAFLKIEDLCKVYGKNENKVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            complement(34789..35778)
                     /locus_tag="MJE33_001822"
     CDS             complement(34789..35778)
                     /locus_tag="MJE33_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY9647758.1"
                     /translation="MFRNREFRKFAILFLLITILTVALGFAISIMTGILSIVSATTFG
                     IAFFVFTKNRYKSIAQISEQINLVLHNANHLYIAESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIR
                     MDWLITSLLKLSRLDAGIVVFQKEQIDVNNLISSALHQLLIPMELHNITLHIDIPKGV
                     RILGDLNWLSEAIQNIFKNCMESVGDNGKIDIICEDNFLFTQLTIHDNGAGFKKEDLP
                     CLFNRFYRGKNSSTAGYGIGLALCKTIIMRQGGTITAQNHPQGGAIFVIRFPK"
     gene            complement(35765..36451)
                     /locus_tag="MJE33_001823"
     CDS             complement(35765..36451)
                     /locus_tag="MJE33_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY9647759.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKEKQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            complement(36951..>37232)
                     /locus_tag="MJE33_001824"
     CDS             complement(36951..>37232)
                     /locus_tag="MJE33_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647760.1"
                     /translation="KSLRENGIEADIVNRIEEPRPNILDAIRNKQVDIVINTPTKGND
                     STRDGFKMRRTAIEFSTEIMTSLDTLKALVEVKKKHLNKDKLKVYNIAE"
BASE COUNT        11910 a         6895 c         4170 g        14257 t
ORIGIN      
        1 tttcgtcaat actttttttg ttttttattt taaaaaaggt tataactttt ggttttttta
       61 ataaatataa ttaaaactaa tagatataac ccttgatatt acaaacaata catgcatata
      121 taattttacc tattttgcaa ttttattttc ataagaatat gaattagctc tcactctatc
      181 tgcccaaaca tttatactct taactttatt aacattagtt gtaccctcaa aaatatccat
      241 tgcatcctta caaaaatata tcataacatc aagaaccaaa aatccaacac aaaataaaca
      301 taaaaaatta attacttgaa acatcagaca ttcccccaat aattttttat atatagtatt
      361 ctcataaatt attcttttta ccttctcata aattattcta aatattttca taataaatta
      421 ctttcaatca tattttctaa atataaagtc tcaatcaaca aaaactaata tcaatttcta
      481 acgtttgttg gtaatttata aattaaatat ctaaaagaat ctttgaagca tataaaaagc
      541 attccacact gacattttaa ataaaattta gctatttatt gataatttaa tagctaaact
      601 ttatttaaaa cttagtagaa tgctaatatt tcttagtttt cttcttcttt atttggattg
      661 ttatcaatag gtttcatagt cgggaataat aatacatctc ttatagatgg agagttagtt
      721 aataacataa ttactctatc tatacctatt cctagtcctc ctgtaggagg tagacctacc
      781 tctaatgctt ttaagaaatc ttcatccatt tcaaaagctt catcatcacc taattctctt
      841 tttctcaatt ggtctataaa tcttcctttt tggtcaatag ggtcattaag ttcagaaaat
      901 gcattagcta actcccactt attagcaaat gcctcaaatc tatcagttct tcttgggtct
      961 tcaacatttc tcttagataa tggagatact tcaactggat ggtgagttat aaatgttggt
     1021 tggattaatt tatcttctcc aaattcttca aagaaagcat ttattacttc tcctcttctc
     1081 attccaggtt ttatctctat acctttttct ctagcaactt ctagtgcctc ttcatctgta
     1141 tttatagtag aaaaatctac accagaatat tctttaacac aatcttccat actcattctt
     1201 ttccaaggtg gtgtaaagtt tatttctgtt ccttgatagt taactatcat acttcctgta
     1261 gcaacttcag ccatatgtgc aataacattt tctgttattt ccatcatatc agtatagtct
     1321 gcatatgctt gatataattc tattgcagta tattcaggat tatgttttaa atccatacct
     1381 tcatttctaa acattcttcc catttcataa actttatcaa atccaccaac aataagtctt
     1441 tttaaataca attcattcgc tattcttaaa tacattggta tatgtaaagt attatgatta
     1501 gttataaatg gtctagcatt tgccccacct gctatagtat ttaatattgg agtctcaact
     1561 tctaaaaatc ctctatcatc taagtatgct cttaatgctt ttaatgcttt tgttctaatt
     1621 aaaaaagcat tttttacttc tggatttact attaagtcaa catatctttg tctatatctt
     1681 agttcttggt cttttaatcc atgatatttt tctggtaaaa cttgtaaaga tttacatagt
     1741 aatactacag atttagcctt tacagatatt tcaccttttt tagttttaaa gatttctcct
     1801 tcaattccaa ctatatctcc aatatcatat gttgaaaata cactatattc ttcctctcct
     1861 atagcatcta gtcttacata agattggatt cttccctctt ggtcttgaat atctataaag
     1921 ccagcttttc cttgtattct cttgctcatt atacgtcctg ctatttttgt agttttacct
     1981 tcaagagaat caaagttatc ttttatatcc atagaataat gagttctatc atatctactt
     2041 tctttaaaag gatctctacc tgactcttgt aattttttta gtttatctct tcttacttga
     2101 agtacttcac taaggtcttc ttgaatttgc gcttcttcat tgctttgttg attatttttc
     2161 atttttttcc tccaaattat cttcttattt ctaatatttc aaacttagca actccatcag
     2221 gaacttgcac ttccacaact tctttcgcag ctctacctaa aagtgctttc cctacaggag
     2281 attcgtttga tattttacca tcatatgggt ctgcttctgt cgaaccaact atagtatatt
     2341 caacctcttc atcaaattct aaatccttaa ctttaactat tgatcctatt gtaacaacat
     2401 taaggtctat tttgctttca tctatgataa cagcttttct aaccatattt tctaatttta
     2461 aaattctttc ttcaacttgt gcttgttcct ttttagcttc atcatactca gcattttcag
     2521 ataaatcacc aaaagatata gcaactttta ttctctctgc aacttctttt ctctttacaa
     2581 cctttaagtt ttctaattct tcttctaatt tatcatatcc ctcttgtgtc aataaaaact
     2641 ctttattttc ttccattata aatacaactc cttcacaaat ttcttaggaa ttttcctaat
     2701 tatattttaa aaattttata atcttttttt aatttatgcg ctatattata agtcactatt
     2761 atttttatgt caaggagtct tccatatagt actccaattt cgacacaact tcttgatagt
     2821 cttctatttt gttaatttca tcccttaatt ttgcagagtt tctaagtcct tttaaatacc
     2881 aagcgatgtg ttttctcatt tcccttacag ctacatactc tccatgttct tctactgcaa
     2941 gttttaaatg cttaatggca gtacttattt tttcgttaag agttggctct ggcaaaatct
     3001 cgcctgtatt catataatgg tttattcttt taaatatcca agggttacct tgagcacccc
     3061 tacctatcat tatagcatca caaccagttt tgtctaacat atttattgaa tcttctattg
     3121 taaatacatc accatttcct ataacaggta tactcaaatt tttcttaatc tctgcaatta
     3181 tatcccagtc tgctttgcct gtataaaact gttctctagt tcttccatgt attgctaaag
     3241 cacttattcc acaatcctct gctatctttg ctatttcaac agcatttaca ctattatcat
     3301 cccagccttt tcgaatcttt aaagtcactg gcttttttga gttcttcact actgccctca
     3361 atacctcttc tgcaagctta ggatttttca taagtgctga gccatctcca tttttaacta
     3421 cttttggagc tggacacccc atatttatat cgagaatctc atttgaataa tcattcatta
     3481 tctctgctgc tcttcccata aactctggtt ctgaaccgaa tatctgaact gctacaggat
     3541 gttcttcctc ttctatctta agcatttttt tagtattttc atcatcatag caaagtgcct
     3601 taccatttat catttctgta tataaaagcc cacatccctg ttctttacaa attaatctaa
     3661 atggaaggtc tgttacacct gccataggtg atagaaaaac tttatttttc aattctaaat
     3721 ttcctatttt cattgttttt ctcctatttt tgcctttatt ttgtgattta actttaaatt
     3781 cttatttcct cattataata taccacatat agctagaaat ctatcataaa aagggaattg
     3841 gatatataac ccaattccct gtatattaat tattcttggt ttttttcata tattattctc
     3901 aatccttcta gtgttaaaaa accatctaca taatctatac ttttcgtctc tgaagcaatc
     3961 agtttagcta atccacctgt agctataacc ttaacatcat cacaattcaa ttctttcttc
     4021 atcatactta ttattttgtc aactaaacca acatatccat aaattattcc agattgcatg
     4081 gctgatacag tactcttaca aatagtcata cctggcttag ctaattctac tctaggtaat
     4141 ttagacgcac tttgaaataa cgcctcacta gatattttta ttcctggtgc tattgttcca
     4201 cccaaatatt cacctttttc agagatagca caaaatgtag ttgctgttcc aaaatcaaca
     4261 agtatacttg gtgctccata cttttctatc cctgctacag cattaactat tctatctgcc
     4321 ccaacttgtt ttggattatc atattttata tttagacctg tttttatgcc tggacctact
     4381 attaatggct gttttttaca gtactttata caaaagtttt caagagaatg cataacattc
     4441 ggaactacag atgatattat aacatcatca atatcactta tattcacatt atcataatca
     4501 aataggttac ttatcaggat tccatattca tcagacgttt tttccctatc tgttttaatt
     4561 ctccagtaat taactaattt atcaccttta tatataccta aaaccatatt agtatttcca
     4621 acatcaaata ctagaagcat atgtcattaa ccttctttct attttttgct tttcttaagt
     4681 acagtggcaa cagcagctac tactaaagcc cctactattg cttctggcac cccatttgct
     4741 gcggcaagag ccattattac ttttcctgct gaaccacttt tacccattgc atgaagatac
     4801 ttgtcagcat ataagacata tatcattcct aaaactccag ctgtatttaa cagagaacct
     4861 attgcccctg taattaaacc tgatatgtat accttatttg tagccttaat tattaactga
     4921 tatacataat aagctaatac acctatcata actcttggta atattgaaac taatggattt
     4981 ataaaaacaa aagatgttac tgttgggttt gttatagcat ttaagagact tgatatccca
     5041 aatataaatc ctattgctgc cccaactatt ggtccttcca taatagctcc aataataact
     5101 ggtatgtgca ttatagttgc ttttgtaggt ccaactggaa taaatcccaa tggagtcata
     5161 gatagcataa tagaaatagc tgatagtact cctattatag tcatctttct tacatttact
     5221 tttcttactg atgcacttgt gttcatcata aaaaatacac ctccgttcca gctcattaag
     5281 atgccggact acttaatttt ttaatttttt cgtttttttc ttataattat gagtcaagct
     5341 accattgtag gatgcccgct ctaacaagaa taattttatc aagatgagtt gccattgtca
     5401 acaatttgac aagaaaaata tgctaatttc atcatatttc tcgttttttt actatatctt
     5461 tatatgccat tatattttct acactatccg ctttttttac tgcatttatt atactttttt
     5521 ccacaacctc tacagccaat gaacctacta atgttatatc tgcttctatt tctcctgttg
     5581 atattgtgaa tattgtatct ccatcatgag gtgtatggat aggaaaaata gacttagcat
     5641 atccatcatg tcccatttga gatatctttt tacatccagc tttggttaac ttagcatttg
     5701 ttgcaattat cccaattgta gtattatcta cactaaatcc acctttactt tcaccttgtt
     5761 tcattaattt atatgtatca attgtttctg tctttttatc atttaaagca ccagctataa
     5821 ccttaccatt ttcataaaca tctccaagag catttacagc aattaatgca gatacaacta
     5881 gaccattatc taattttata gaatagctac ctattccgcc tttcatagca tattcctgac
     5941 ctcttatctt tccaacagtg gctccacaac ctgctccata atttccttga tttaaaatat
     6001 cagtatttgc aacttcacaa gctctataac ccatctttaa atctggtcta catttaaagc
     6061 tccctactcc taaatcaaat aaaactgctc ctacaactat aggcacttta cctacaccta
     6121 catcaaaacc aattcctttt tcttctaagt atctacttac accacaagtg gattcaagac
     6181 catatgcaga acctccagcc aaaactactg catgaacttt ttgaaccata tttacagggt
     6241 ctaataaatc ggtttctcta gtccctggag cagagcccct tacatcaaca ccgcaagtag
     6301 ctccttcttc acatattact actgtgcacc cagtaagacc atttctatcc tcaacctgcc
     6361 caacctttaa gccttctata tccaaaatat tattatacat atcctttttc tcctcttata
     6421 gaaacttcac ctgaaataat ttcttttata cttccatcac ttctttgaac taataaattt
     6481 ccctcttcat ttatgtctaa acatttcact agttctctat tatctccttt taatacatat
     6541 atatttttat ctataattgc agaataatct ctacatattt ttaaggtttt ttctttggaa
     6601 ttatttacag tataatctaa atataatttt tcaaattcag tcaatacatt cctaacaata
     6661 tctactctgg ataacatata cccttcttta tacaatgatg tggctttgtc agctatttct
     6721 tgttcaaaat ccattatttt aacatttatt ccaattccaa gaacaacata atttgttctt
     6781 tcaatttcag cagataactc tgttaatata ccacatattt tttttccatt taaaataaca
     6841 tcatttggcc attttattaa tgtatttgct cctagattgt ttaaagcttt cgttacactt
     6901 gctcctgcta ttaaagttat aaatggagct ttgaatggtt ccatatttgg ttttaatata
     6961 atactcatcc agattccttc atgaggtttt gaatcccatc ttcttcccaa tcgtcctttc
     7021 cccatgtctt gatgttcact taaaatcaca gtcccatcta cactatttat agctatcctt
     7081 ttagcatgtt catttgttga atctatagtg tcaagatgtt caatattttt tccaataaac
     7141 tctgtatcta atttatagta aatattctgt gggcttaata aatcagttgg attttccgct
     7201 aatcgatacc cttttttatt tacagattct atattatatc cctcttcttt taaggctttc
     7261 atatgtttcc atatagctgc acgtgacacc cctaattgtt ttgataattc ttctcctgaa
     7321 ataaattcag atttattatt taatataatc tctattattt tatctctcat atttcacctc
     7381 taatatgaca taatttcatg acttattttc ttctaataaa taattataca ttattaatat
     7441 acttttaata ctattaattt taaatttcct ttttaattat tataatggtt gttttatatt
     7501 ttatattgat gatttatata attttacatt ttatttaaat aattttaaaa taataaaaaa
     7561 actgaaagta tcaattaaat taatacatgc aaatataatt tgcttattaa tttaatatta
     7621 tactttcagt tttagtattt aatccacatc agataataat cttaaaatga ccaaggtctt
     7681 aaaactattt atctttatca gatttatctt ctaaattttt atttatatca ataacattgt
     7741 ttctttcttc ttcatctttt tctaatttta tagttaattg ttcttcaact tctttagaag
     7801 aattttcctc tgtaactgca ttttcaattt cattcagagg taactcttta ttaaatgctt
     7861 ttacaaattg ttctgcatct aaagtttcat atataagtaa agcttgtgct acatactcta
     7921 atctatccat attttcttgt aatatacttc ttgtcttatc atatgcacca tcaactatac
     7981 gctttgtttc ttggtctatc tcaaaagcaa cttcttcaga ataatttctc ttacttgaaa
     8041 aactattacc taggaatact tcatcatcac tgtcaaatga cattggacca agtttagaac
     8101 tcattccata ctttgttacc atacttcttg cagtagctgt aactctttct aagtcatttg
     8161 aagcacctgt agatacatcc ttaagtacta gctcttctgc tactctacca ccaagtaaaa
     8221 ctacaatatt ttctttcatt tcattttttg tagcatagaa cttatcttca acaggaagtt
     8281 gcattgtaaa ccctccagct cttcctcttg gaactattgt aacttgatga acaggactta
     8341 cttcttcaag tacatgggca caaactgcat gccctccttc atggtatgct gtaagtcttc
     8401 tttctttctc acttattact ttcgattttt tagccacacc tgctataacc tttgttatag
     8461 cttcttctat agtttccatc ttaattttct tttctctttt tctagctgtt aatattgcag
     8521 cctcattcat taagttctct atatcagcag gagtaaatcc tggtgttctt ctagccaaga
     8581 catccatttt tacatcatca cttaatggtt tatttcttga gtgaacttta aatatagcct
     8641 ctctaccttt tacatctggt gtaccaacaa caacttgtct gtcaaatctt cctggtctaa
     8701 gtaaagctgg gtcaagtata tctggtctat ttgtagctgc catgattatt ataccttgat
     8761 ttaccccaaa tccatccatt tcaactagaa gttgattaag agtttgctct ctttcatcat
     8821 gacctccacc aagacctgca cctctttttc taccaactgc atcaatttca tctatgaata
     8881 tgatagctgg agcacttttc ttagcctgtt caaataaatc tcttactctt gaagcaccaa
     8941 ccccaacaaa catctcaacg aaatcagaac cacttatact gaaaaaggga actcctgctt
     9001 ctcctgcaac tgctcttgat agatatgttt taccagttcc aggaggtcct accataagca
     9061 ttccttttgg tattcttgca cccaattcaa tatatttctt aggattcttt aagaaatcta
     9121 ctacctcctg caaatcttct ttttcctcat ctagtccagc tacatcttta aatgtaactc
     9181 tagttttttc atcatcttta tgaaccttag cttttgattt gccaaaactc ataacctttc
     9241 cgcctccacc ttgagactga ttcataaaca cgaaccaaat aatcaccatg aagaatatta
     9301 acagtaatgt aggcaacatt tcgacaaacc aaggtgtaga tggcttagct tcaccaccaa
     9361 aagttagttt cccagcctta gcttggtcta acacctcatc tgcaagttta tttcccatca
     9421 cttctgttgg aacatatgac ttaaactttg tatttgtatc ttttatagta ccttctacag
     9481 aagtttgatt tacaaagtat aatcttgata tattctcatc tgttaattct ctatatactt
     9541 cagagaattt taaatctttt attttttctg taggcttacc tgagaattga actataccta
     9601 ctataattat aaaaacaagt agataaaaac ctgccccttt tagcaactta ttcaatatag
     9661 aagccctcct ttcatacatt gctttgccct ataatttgtt aaaattaatt tttaatactt
     9721 cttttgtgtt ttcatcaatt ttataatttt cactgatttt ataatctcca acacatatta
     9781 ttccttcact atctgtgatt ataggaattt tacatctttc ttctcttggg atttttaaat
     9841 ctatgaataa atctttcact tttttacttc ccatagcaag ctttatctta tcaccttgcc
     9901 ttctgcttct tattactata ccccctttta ccttattaaa atcaaaccct ttagatgaat
     9961 tgtctaattt catactctta tacctatcta tgctcattac ttgggtctca ataatttgtt
    10021 ttaattcttt tattttaata aacccattac taggtacatt gtaataaaat tcaatttcct
    10081 cattaacaat ttcctcattc gttaatatta tactgtcttt ttttctataa acaaatatat
    10141 ttcttggaag agttaacatt ttatttactt ttgaattatc ttctaaagac attatatctt
    10201 ctatatgtct ttggtcaaca aaattagtat ctcctagtat atgttttatg gaatttctaa
    10261 gaactctaac tttgatagca tcatgtaagc aaacaaaatc gtctaaattt atctctacaa
    10321 agcctttttc ttttatattt gaaacttctc taaatttagc ttctgcctct ttctcaatat
    10381 aatcgttatc actttttaaa ctattgctca tcctcactat agattctatt acatttgaat
    10441 taaaattatc cttcatatat ggtaaaaggt ctaaccttat cttatttcta gtgtatatat
    10501 tttctaaatt tgttttatct atcctcggat ttaaattata agcttcacaa tactcctcta
    10561 tctcatttct ttccacgtct aatatgggtc taattatgca attatctcta atataatcaa
    10621 ttccctttaa accttttaaa cctgttcctc tcatgatacg catcataacc gtttcggctt
    10681 ggtcattgag attatgacct attgctattt tattggcttt aatcttatcc ttaatttcat
    10741 aaaacatttc atatcttagc ttccttgctc cttcttctaa tgataatccc tcattttcac
    10801 aatattttgg tacattaata gactttacaa agaatataat tcccatatcc tcacaaagtt
    10861 tagatacata taatgcatct ttttgagctt ctatccctct tatttgatgg tttaaatgtg
    10921 ctgcatatat ttctatatta aaatcttttt ttaatctatt tagtacatgc aataaacaaa
    10981 ctgaatcagg tcctccagaa agacctaaaa ctattttatc gcctttttgt attaaattat
    11041 gtttatttat agtacttagt accttatcaa atatcatatt tatccctctt acacttagtt
    11101 ttattcactt ttaagtatac tttctataaa aaaacgctaa acccttgtat caattttaag
    11161 aaattaaagt ttaatataaa tataataata ccaaataaat taaataaaag gtagtttttt
    11221 cactaccttt ataaaatttc tggaaaatag ccatagttat tatttagtac ctatgtatat
    11281 atctatttta ttcctaccta aaaataaatc ttgtaccttt atattatagt ctattttcaa
    11341 ttttatgtgt tccccttctt gtatatttat atctaattct cttgtatcaa tatcaattaa
    11401 taaagtacct tgtggggtca catactttgt agtactactt tctccacatt taaacagcat
    11461 tgttgagtta atagcaccaa actttttgat actaacttga tttttgagta tctttatagt
    11521 attagttatt tctatatctt cttctttttc cttgtaaata acatatattc catcattttt
    11581 ataaaaaatt ttaccaaagg cagtcatctc tatggtgtcc atatttcctt tttcatcata
    11641 ttgtctagta tttatactta tttttacact caacgatttt tcctccatag attcttcata
    11701 taaactagta tttttgtatt tctacttttt gaatcttatc aatttttttc tttaagaact
    11761 ctcttgctat atctgaaaat ttttcaggta tatctgatac ataatattga taagttccat
    11821 gaatttctgt attgtttata atattttgca cctctaaaat cttttttaaa tcctttgcag
    11881 tctctttagc tggattaaca agttttatat gctctccaac ttcttcacct attgttctct
    11941 ttaaaatagg ataatgagta catcctaaca ctaaagaatc tatatttttt tctttcaatt
    12001 cttgtaaata tatctttgct gtcaatttag ccacttctgt atttgcccat ccttcttcaa
    12061 caataggcac aaataaagga catgctttat taacaatttc tatactctca tctattttag
    12121 atatttccaa gttgtatgct tttgaactta tagttccttc agttcctatt attcctacaa
    12181 ttttgttttt tgtagaactt acagctgttc tagctcctgc ttctataact ccaattatcg
    12241 gtatatcata tttttcttga gcttctttta agcttcttgc agtagcagtg ttacatgcta
    12301 taactatagc ttttacacct tttgttttta aaaagtttat agcttgaaaa gtatatttaa
    12361 ttatagtttc tttggatctt gaaccatatg gtattctggc agtatctcca aagtatatta
    12421 tatcctcatt tggtaatatc ttcattatct cttttagaac tgttaatcca cctaatcccg
    12481 aatcaaaaac tcctattggt ttattgttca tctttataca acacctactt tacatttttg
    12541 tgtttaacaa caacttattt atcgctaaaa tttatattta tattcataat taatatatta
    12601 ttacctaaat gattattata gtttatttta cacaaaaaat aaaggagcta tcttaaatta
    12661 gaaaataatt tctacaaaaa gacaactcct ttcactactt aaaaatttaa atttattttg
    12721 caaacacatg atttcctatt ctcatataag gattcaatct atttatccat ttgcaagtgg
    12781 ctatttctgg attccaaaaa tatactgctt tattagttgg gtcagttcca taaagagctt
    12841 cctgagctgc tttacgtgct gagtctgttg gtgccatctt tattgaacca tcaagcacaa
    12901 ctgagaaagc atttttttga tatatgactt cttctattgt gtctggaaat ctagggtcca
    12961 atactctatt tattactaca gctgctacag caacctgtcc ctcatagctt tctcctctag
    13021 cttctcctgc aaccaactta gataataaca tcaagtcatt atcagataaa tttattactt
    13081 ctttctttgc tatatttttt tcttgtttgt ttactttctt aatattttct gttggttcat
    13141 atgcaaatac ctctttaata ggcataaata taaaagtcaa agctaatagt gtagccatca
    13201 gtttactcca cctgtccatg attaaaacca ctccttacat tcttattttt aaaatttttt
    13261 ctctaaatcc ttgactactt caagtacttt aaacttaatt tttactctac ttgttttttt
    13321 tggtgtatct tgttttatta acttatattc tttatcagtt aaaacttcct tcaacttatc
    13381 atctgtagat ttatctatca ctccattaga ttcatcaaca aaacatagtg gagggaacat
    13441 aacacaccac cagttttttc cttcaccttt cccaatgata atctttaatg ctttatattc
    13501 accagctgga agaactatat tagaatattg tttagtaggg aaattgctat attgtatacc
    13561 tacctttaca ctgtaattat atccattttt aagaataata tttttactta tttcttccag
    13621 actactatat tcctttttta taatagcttc actttctttt atactttttg aattttgaag
    13681 ctttggctgt aaatatttta ttacttcatc tctaactttt agtttaagtt cttggtcttc
    13741 atctgtattg ctgtttgcta taacatgaaa tcttattaat ttatctttat aatcttttga
    13801 tatattgtct acttttttta cctcaccatt tattactatt gtcattattg atatgactga
    13861 tataagactt aatattaata gtcccaatct aatcttaatt tttctcattg aaaattcccc
    13921 ctcaagataa atttatatca tcaattatgt ccaattaaat ttaatttatt cataatattt
    13981 tttaatacta ataaaaaaag ctatcttaaa atgattacaa tcattttaaa atagcttttt
    14041 aaaccttata ttttataagt ctttactaat catagcctta tctatcataa actgctctgc
    14101 tttttctata tgctctgtag catacttctt ggatagctca ctatcacctt taattatagc
    14161 atcaagtaag tatttatgct cttcaacaat attatgtgat gtatcataag cagacatata
    14221 ggtaactcta aatctatata cttgttccca caatgagttt attaattgga ttaatttttt
    14281 attattagtt gctttaaata tacattcatg aaattctaca tcaagtttta ataacgcatc
    14341 tacatcggat tccaaagtgc tttttttaaa ttcttctgat atatctttaa gctttttaat
    14401 atcttcatca ctaattcttt cagcggcaag atatgctgct aagccttcta ggcttgctct
    14461 tacttctaat acatccataa tatcctttaa tgacatattt gccacatacg caccttttct
    14521 aggcaacata acgactaaac cttctaattc aagctttctt attgcctctc tcactggagt
    14581 cctacttact cctaattgtt ctgctagttg cacttccatt agcctttgac caggctttaa
    14641 ttttccttct aagattgctt ctcttaaatt ctcaaagact acatctctta atggcttgta
    14701 attatccaag ttcagtttag ttaaattctc cacaaatttc aacccctttt tgactgctat
    14761 taacaacata cacctgttta tattttttta ataattcttt ttttccaatt agagcatctt
    14821 ctttattttt aaatagtcca aaaactgtag gaccacttcc actcatcata gaacctaaag
    14881 cattattttt catcattact tgttttatat cacttatttc tttgtgttta cctatagtaa
    14941 cattttcaag aatatttacc atgctttctg atacagcttt tatatcttca gattttaaac
    15001 actctatcaa atatttatta tttggtcttt ttttaatatt attcaaatct aatccttgat
    15061 aaacttcttt agtagaaacg aataagtctg gtttgcatat caatatattt gtatcacatg
    15121 gtaaaccttt gatttttgtt agcttttctc caataccttg agctaaggca ggtcttcctg
    15181 atatacaaaa aggaacatct gcgcccaaat taagtcctat ttcttttaat tcatcttcag
    15241 ataatctaag ttcccatagg tgatttaaac ctactaatac tgcggctgca ttagaacttc
    15301 ctccagccat ccctgctgct acaggaatat ttttttctat gaatatttct acaccttttt
    15361 ttatataaaa tttattttta agtattttgg cagctttata aacaatatta tcttcatcta
    15421 aaggaatatc taaactagtg ctttttacct ttatttcatc ttcgtctaat tcttttatct
    15481 ttactatatc gtataaatct atcgtttgca ttatcatttc aacaaaatga taaccgtctt
    15541 gcctttttcc caagacatca atggataaat ttatttttgc tctactcttt agtcttataa
    15601 aatccattct atcctccttg tttaaagaat aatgcatcac taacaataat attatactac
    15661 aaaaaataaa ataatagggt attataatac cctattatac ttaattactt taaatttttt
    15721 aatattaatc tactaatacg actttttttt ctaaaataag acacttacca ggcttaattg
    15781 gttcatcaag ttttatatca ttaagttctg caatttcatt ttctgttgta ttgtattttt
    15841 ttgctatatt ccagaaagta tctccttctt tgcatatata tacgattatg ctaggagctt
    15901 tagataaatc atatacacct tggtcttctc ccttgataat aaaattttct gctttcttat
    15961 ctaatgcttc agtaaatctc ttaattttga taatcaagtc tatctggtcc ctattcaaat
    16021 caacttcgac tttatcaata catgctgtat taaacacaga tgctgtatca gttagattat
    16081 ccatttctat atcatgttca aatggaattt cctcactaat tttataaacc aattttaaac
    16141 cttcaactgg gacaaataaa atttcaactt ttatgattcc ttgtataatg cttttatttc
    16201 cttcaatata actattttct atagacatgg ttggacaaac gcttacaata tcttttattt
    16261 gtatatcatc attatcattt cttatgcctt ctctaaccat aaatgtttct tcactacgtc
    16321 ttaaagtctt atttaattga ataggtttgt ggtcaaattt tattatcttt tgaggcgaat
    16381 atgcatcttg taatacttct cttgtaactt catctgtaac ttttacctta caacaagcca
    16441 tacaatctat ttctaaaaga cctgtgttgc tttcactatt ttgcttgaaa atatgattaa
    16501 aatctgacat agacaacaat acttcttcag tcataccatc actaactcca gggacttcta
    16561 caaattgagt aaactcaata cctactctat ccaactctac aagttcaccc tcatatgtac
    16621 aagctaacgg atttatttct aatactccac caataataac tttattgtca gtcactctac
    16681 tttcttttac tttaacacat ggatttaaac ttattataga ttgaatttcc tctgtgttta
    16741 ttgttatagt atctcttata gaactttctg ctttttctat tccaactata tcttgaaaac
    16801 aaatttcctt tctgtgcttt tgtatgcctt ccacttgcgc cacatctttt acaatgtcta
    16861 acctttgctt ttcaaacaaa cttcccctta tattcataag agctcctact ttaatctttc
    16921 tctcattcat tatagtacaa tccatatgct ctacttctga aaataacatg tattccatat
    16981 cttgaacaac attgtctttt tctattactt cgtttatatc tacttttccg tctacattag
    17041 aaatagtatt tttatcatct gcaatataga ttacattata attaaaacta cctctgcaaa
    17101 gtatcttccc atctgccatt tcaatttttt taagtgatat gtatccttct gtttttacaa
    17161 tttcatatac atctaatttt ttgtctggta caactgcctc cgcttcaatg aatgtttgaa
    17221 attttccaaa gtctattcta ttgtcaactt taattacatc tttaattaat tccatagtct
    17281 acctccttaa gtattataaa aatatcacac tatatttata tttaaggagc aaaaaaaaaa
    17341 ttacacctat ttttaaatag gtataatttt aacttatttg taatttatct tgatctctaa
    17401 aaacttgcaa ctttacatta gatgttaata aatctgagta actataagat actctaggat
    17461 atccattact ttcatcatct aactttataa caaacacgct tggataaact ttttctagta
    17521 taccctcttt tgtaataatc tgctttcttc ctttattagc tttcaacaat attttctttc
    17581 ctatatgtct ctctaagctt actcttatct tatctagagt ttgaacagta gccacaatat
    17641 caccttcttt aacgtttata tgtttacata atatcacata taaacatttt tgtcaaatgt
    17701 ttaaactaat tattttatat attttttgca aaaatgtcaa tactattttt gaagatatac
    17761 agtataatta tgttaatttt tgctaatgta atattgtttt tggcattgcc catctaaatt
    17821 ttcctctggt ttcagaacca ccaattccct taacacctgt aatagtctgc tctataacct
    17881 catcaatagg aactactaca tcaattcttg aacaagcaat tttctccact ataaaaacag
    17941 ggtctaatgc atgtatcatt atccttccag gcgaacttgc atagtttgct cctgcactta
    18001 ttattttttc ataatttgat tggcatgctc ctgcgaatat gacaagatta tccaaatttg
    18061 gttgccattt acgtgcttct tttactgttt taacaaaatt tgaagagttt ctatagttac
    18121 tcatatcttc tatatctcct ctttttattg tcattgcatc atgacctgtt attactagta
    18181 tatccggatt atatttttct agtagtgctc ttacttcttt atactgattt tgctctgaaa
    18241 cagctacacc tactgcaggt attccaagtt gagcatatac atccaagcaa atttttaaat
    18301 attctttatc tccatctatc tgtagtactt ttccaggcat tccatatgtg ttaggattta
    18361 attgaagttt tggaactgct ctagtcattc ttttttgtct ttctttagct tttcttactg
    18421 acttatataa taaattttct acatttttat ctattaatat atcttttata tcaggtgctt
    18481 ttactaattc taggtcatca atataagcat ctgctattac tctaaatgca attcccttta
    18541 gtatagctat tttctcatta ttttcatcta caccaaaaga gactatttta aatactatat
    18601 ctttgttatg tgactttcta gctacaatgt ctcctacctt catagttaaa cctccaataa
    18661 atttttatct tagtttatac tatgttcgat ataaaacttt gtttcctaat gtcaaaaaaa
    18721 catttcttat aatacttctt ttatcactct taaagcattt ttatagtata tcttttctat
    18781 atcatcttca ctaaaccctt ctttttttag aggttcatac agtttaccca tctgagatat
    18841 atcttcaatc tcaacttttg aatctattcc atcaaaatct gaccctagag atacaacatc
    18901 tattcctcca acatttacaa tatgttttat gtgtctaacc atatcttcaa gtttactttc
    18961 acttttatca cttaaaaata agtggaagaa gtttatacct gtaactccac ctttatttgc
    19021 taatacttta atcatatcat cagttaaatt tctagaatga ttcatcattg ctcttgaatt
    19081 agaatgtgta gcaattattg gtttagaaga aatctttgca atttcataaa atccaccatc
    19141 tgaaatatga gaaacatcaa ccaacattcc caattcattc atcttatgta ccacttctct
    19201 tccaaaatca gtaagccctt tttctctata ttctacttta ttatgaggaa agctcaattc
    19261 attcacatga ttccaactta ttgtcatcat tctaacccct aagtcgtaaa atttctttaa
    19321 attttctaat ttaccttcaa gtactgcacc ttcctcaata gataaaagag ctgtaagttt
    19381 accttctgat tggtttttca taatttcttc atagttagta gctaaagcta tttcatcact
    19441 attttttttc atttcctcat gaaatttatt tgccatactc atgcaataat caaaaggatg
    19501 ttttacttct tcagtatcaa caaaaagagc aaatgtttga gctaatgaat cccccttttt
    19561 caatctgtct atatcaacag aatatttatt agacttaagt tcacttgttt ctacattttc
    19621 cattaatttt gctatagtat cacaatgtaa atcaataaat ttcatattta taccccctgt
    19681 atattttata tttatatcta aaaatagaaa tagttgtcct tatagacaac tattgacaac
    19741 tattttttaa tattcttttt aatattactt aatcttattc ttcttttaag ttaggtatta
    19801 cttttttaac ttcttcaatt tgatgccatg ttttatctat attattttca ctaatatatt
    19861 tttttaaatc agttaaagat gtaaaatctt taactgataa attgtcatca tcatctgcaa
    19921 atcttaaaat aaacatgaga ctcccttact ttccacaaca tttcttatat ttttttccgc
    19981 ttccacatgg gcatgaatca tttcttccta tttttgcttc tttaactatt gttttagact
    20041 tcttataagc ctttattatt tcttttcttt ggtctgctgg tagtatatct tcccaacctt
    20101 gtaaattata tagccaatga gcctcaacag ttaccatatt gttatataat ttttcccaat
    20161 ctatttttat agaaacttct ttatcagctt ctacttcttc taaaactata tcttctttta
    20221 agctttcact tattccatct atgaatccca tgaaaaattc attagtagtt ccaaattttt
    20281 cagccaattc acttacttta ccttcaacaa catccatttt attatttaat atttcattgt
    20341 atattccagc ttcaactttc aaatattctt cccagaattt tacttctgct tcttgacttt
    20401 catgattatc acttaaattt ctccattcag tatataaact catacttttt cctcctaagt
    20461 atacctatat ttttatagtt tttatagtat aaaatctttt agttagattg gaagtttctt
    20521 ctttttgatt ataccatatt ctatttaaag ttaactctta tattatatca taattaaaca
    20581 attcttttaa tacttttata gcatactctt ggtttataaa ataagggctt gtcgcaattg
    20641 ttattacttt ggctttttta attaagtttt ttatcttgta aaatttgagt tcaaatggta
    20701 tatagtccat atcacttgaa aatatatcca aatctatgtc aagaacaaac tctgattcta
    20761 cttctaaatc aaatccatat agactatcta ttattattaa ttctgaaaat atgttatatt
    20821 ttaaagcagg ttttataaaa cttcccacat ttaaaacttc atttgtatat ctaaatacat
    20881 cttttagatt atttacatct acatcatagt tctcaggttc tctagtatcc ttatgttggt
    20941 ctacatgcac taacttgcat ccttttgtaa actcaccctt taacaaagac tttgcccaaa
    21001 aataaaatgc atggttatga ttgtcaaaca catatatctt tttatttccc aggacatatt
    21061 cgaccatatt ctccaatcct tttgctttta tttccttatt ttcatctact tcattaaata
    21121 ctacctcatt gcctaacctt acatcattta atgtaccatt tataagtttt ggaacgtata
    21181 tgctcttatt ttctctttta tcatatgaaa atacattatt tcctactggt ttttctatat
    21241 aaaatccact atattcataa ttttccataa aactttattt cctccattgt tatataaata
    21301 tataagttat aattttaaaa attaaaatgc acttattgta aatcatatca tctattatag
    21361 aaaacatctt atataactaa atataatatc ttctctcaat atgtattata caataagtgt
    21421 aaaatatatt tatataaaat atactacatt cctataacaa aaccatcagc ttcaattgga
    21481 agataactaa ctcttctatt caactccacc tttccaaaaa atactaccca atatggtcta
    21541 aaaatacttt caattttaac tatttcaatc tctgggattc ctcccataaa cctatgagtt
    21601 aatctcatag ccattttatt tgcctttgct tttactcttc gttcgtcctt atcactatac
    21661 tgcacaatat tacaatccac attttttaca acttcatatg taggaaaatc ttgtactata
    21721 ctaccagaac ctgtagaacc attcactaaa atttttaatt catttatata attagaatat
    21781 ctcctattct tgaaaatgga tttcgttttg tgaattttaa gtgtgataat tttaaactct
    21841 atataatgaa gtctaacttc atgagcatat ttgttcttta atattagctt actaaataaa
    21901 gaattttttc cacttttagc tatctctata gcttcttctt ttgtaattct tataggtaac
    21961 gctcttatat ccataaaatc attctctttc ttattttttt cttgtcattt ttaaaactct
    22021 atatatataa tcaaaaaata ttacaattac aactgacata actccatatg gaattatttt
    22081 tccaactata atatcatttc tagtcaatat accataaaat ataaatcctg ttattaaaaa
    22141 gtacaataat actagaaaac cgtctattcc aagccatttt atttctaaat ctttttttac
    22201 cttagacact aatttagaca tagcagtttc tccttttata gattattttg tgtatttaaa
    22261 ttttcaccta ttctattacc aatcatccat gcaataaatg aaaaaggtat agctatcaca
    22321 agaggctcta actcaaagct ctgaactgga aatattttaa aataaccaaa agtcactcca
    22381 ccaacaatca ttcctaaaat accacccata gatgttcttc ttccatttcc aagaagccca
    22441 cctactatag gtacaaatgc cgaacccatc caaatacttc ctacaaatat acatgcatca
    22501 tatacatttt caaatctaaa tgctacgcta agccccataa ctgctgctaa acatacacct
    22561 attctagtaa actttactaa ttgtttctga gataactctt tttttgtaat tcttccatat
    22621 atatcatttg ctaatgtagc cccagctgct agataatagc tatccaatgt tgatattata
    22681 gaacctaaaa ttcctattat aaatattact cttattcctt gtggtaatgt atctaaaact
    22741 aaagttatat atccagcagc tggagccatt tctggataag tagctcttac tataagtcct
    22801 gtcatagtta acaccatgtc aaaacatatc catagtgaaa aacaggttaa taaggctctt
    22861 cttccaacct taggagaatc tgctgctgaa aatctttgat aaaaagttgg gtctgaataa
    22921 gctccaaaag aatatacaat taacatcaat gctttcatag ctgacatacc ctttgtaggg
    22981 tgcataagct gtggtgcatc tttgagtgct tctgtaagac cttgaaaccc tcctatttgt
    23041 tcaaattgac ctggcataac catagttaaa ccaaatacca tacaaaaaaa caataccata
    23101 tctgtaatgg caactcccat aagcccacca agtactgtaa gtacaacgac tactcctacc
    23161 attattaatc ctattaattc tttatctatt ttccatgcta cgttacctat agttcccata
    23221 gcagttattt ctccaagttg tgcacaatat ataaacatca aaattgcccc aattatagca
    23281 acatgcttgc cataaaaact ttccagtaaa tcaggaactg ttatattggt tcttatatga
    23341 atccttggac caacccacaa tgctaaaggc attctcccaa tatgtgcagc tattgaccaa
    23401 actgcccatg cagatattcc atatatagcc gcatactcta cactacctac tgtaccaact
    23461 cctccatacc atgatgcaac caaagttcct actattaagg accatggtaa acttcgattt
    23521 cctaggtaat aactttccat atcttttaca tttctagaaa aataataacc tattcctaat
    23581 aatataaatg caaatataac tataatccca ttttcccaaa atgttaacat atctaatctc
    23641 cacattctta tattttaatt tttaacttta atatctaaat atgaacaact ctacccattt
    23701 ccataaaaat aggtagagtt atataataat tataagtatt taagtaatct tgttattaag
    23761 caattttatt gtttaaacct ttattttctc ttttatttat tttatttaaa gcataagtag
    23821 caaatatccc tataactgca catactacag ataaaataaa tatatatgta tatcctgttt
    23881 gtccatattt atctaaccaa ttacctgcaa tagtgtaata aaacgcatct ggagaaaatc
    23941 ctataaatga agcaaatcca actactgctc ctgtagtttc taaagatata ttagactctg
    24001 aaacagatgc aaagtatata cctctaaatc caaataaaat tacacttagt attatcatgt
    24061 ttattacagc tatccatata aatcctgtac tcttaggaat aaccaagtaa gcagctgtac
    24121 ttactgtcat taatataaat cctacaaaca ttactcttat agatgaacca actttatctg
    24181 ttataacacc agcaactgga gatgctccca tttttataac ataagttctt attattgata
    24241 aagcagaaac tagagtcatt ggcattacat aaacttctga taagtatgga cttaaataag
    24301 ttaaacttga gaatactaaa taagttgaaa atattataag accaattagc caagcctttg
    24361 gcattgtaac tacttttaac aatgacttaa tacttaatcc ttcatctgat gctccactat
    24421 cttttttctc ttccactaag aaaaatagaa gtataccaca tattatagat gctgcagaat
    24481 ataaccatac tacatatttg aatcctactg ttatttcagc aaatttacca aagaagtaaa
    24541 gacctataaa agaaattaat gtacctgata cacctgataa cccttcataa aaaccaaata
    24601 ttcttccttg ctctgactca cttccttgca ttctaacaac cttaacagag gctgagaaga
    24661 aagttaatat tgttgtaaat ccccataata caaatattaa aagtatcata ctatatgatg
    24721 ggaatgtaga gaaataaaat ccaagtattc ctgaagcaat taaagaaaaa gttattaatt
    24781 ttctaactga gaatctatct gcaaaccatc ctcctataaa ataagatacc attcctacta
    24841 atccatataa actcaataag ttccctattt gtgtatgact taatgctaaa gcttgttgca
    24901 ttgggtcata aaatgatgat ttcatatatg gtaaactata catagctgtt gttccaaatg
    24961 ccaatatgaa tattatcaaa aatcttttaa cattattttt gctttccatt gattctctct
    25021 ccttaaaatt ttattgttta taatctcatt catataattt tattttaatt tttctattgc
    25081 ttgtttaaaa tcatttatta tatcatttga agattctaat cctacagcta ttcttattag
    25141 attttcccca ttcatttgaa ctaatgtatg ctcttcccct aagcttgttg ctattgttgc
    25201 aagctctaaa gaattcatta atcttctact agcttggtct cttgtcaatc cattgatacc
    25261 atccttaagt ttaaatgaaa ctacacctcc acctaaacca ttcatttgag ttttacaaag
    25321 ctcatattga ggatgagatt ttaatgctgg atactttacc aattccacat tctcttcttc
    25381 ctctaaaaat tctgctactg ctaatccatt ttcacaatgc tttttcatcc tcatatctaa
    25441 cgttttcata ccccttaaaa tcatccatgc actcattggt ggtaaagatg ctcctgtaaa
    25501 acaacataaa cttggccatc taactaaatc tattaattct tttgaacctg ctactgctcc
    25561 ccctaaagta tccccatgtc catttataaa cttagtaaga ctatgtatta ctaaatctgc
    25621 acctaaatct attggtcttt gtacaattgg tgtcccaaaa gtactatcaa ctatagctaa
    25681 gcttttattt tcatgagcaa tttctgctat tgtttttata tctactaagt ctaatgatgg
    25741 atttaaagga ctttctatat aaactatttt agtatttggt ctcattgctt cttttacatt
    25801 ttgtggattt actgcatcta caaatgtaac ttcaactcca aatttagata acaactcaga
    25861 catgaataat ttagtgtgtg aaaatattcc atctgaacta actatatggt caccactttt
    25921 taccaatgct aatatagtag atgttattgc ccccatccct gatgtactag atatggcact
    25981 ttctgcattt tcaagcatag ccaacttagc ttctaactct gtaagagtag gattacactc
    26041 tcttgtatac acatatcccc atgtcataca caactcttca aaatgctcta ctgtctttgc
    26101 agcaaatgtt gctgtttgac atattggtgt agccaatgca cctgttgttg ggtctgcact
    26161 atgtccccaa tgtattattt ttgtctctaa atcttgatta atattgttac tcattttgat
    26221 ttcctcccat tttgacttat ttatacaacg ttttccctac caaatttcat caatttagct
    26281 tagtcttaat acattttttc aagtatactt ttttatttgt tataatttct aaaaatatga
    26341 cacttatttt gtttttttac agttctaatt ataaactcta tagtaactat agagtcaata
    26401 tgtttttgat atttttttta caatgtttaa aatagtcagt acaaaaatag gtatatctta
    26461 gaataactaa agtcatccta agatatacct atttttatat cacaatataa ataattacat
    26521 ataagcatta gtttttttat cataaataga tttttcaaga tttatcatct tttcataaat
    26581 atattatagt taaatatttt aatattacct aacatctaca ataaaatata ctcttaaaat
    26641 aaaattatat aattaaaaat actctataaa agctaaaaag tttttctatt cgctacatct
    26701 aaaaataatt agatagaggt ataaatgtat ttagtttatt tatacctcta gtcttttata
    26761 aattatatct ctactaaatt gattttttag cacgaataag tgtgctcttg gagataccag
    26821 tcatcttcac aacctctcta tatggcttag aatttaataa ccccaatgca tattgtatct
    26881 gttgattgct gtatttcttt ggcctaccat ctttaaagtc ctttttcttt ttagcaaatt
    26941 cttttcctaa ttgagtacgt tctattatca tatttctctc aagttctgca actgcaagta
    27001 atgtagtcaa aaagaattta cccattgaag tattttctaa aaggcctata tttaaaatat
    27061 gaacacttac ttctttagca aataaacctt ctattagttg tattccttct gatgtacttc
    27121 tagctagtct atccagctta gttgcaacaa gtaagtctcc cttttttaga gatgatataa
    27181 ttttttcaaa tttaggcctg tttgtagtag tgcctgtata agtttccttt attattttag
    27241 catcattata tttattcaaa atttcttgct gttgctgata caaactgttg ttgtctgttt
    27301 gattttttgt acttacacgg caatatccat atataataat catcacttcc taacataaag
    27361 attttgtaca gaagttaccc agaagcttgt ttttacttat ctttatccat tttactaaaa
    27421 cgatttttgg gtgtcaatac tgttaacttt tgacaccata ataattcctt tataatttgt
    27481 ttgactatgt caaaaattac caatataatg aaattaatga gtttatacaa attggaggaa
    27541 ataatatgaa tagtctgtta aaagaagcaa tagatgaatt aaaaaacatc cattgcgggg
    27601 aagttttttt attaaaagaa ttgttcaaag gctatgaatg gaacaggttg gagatgaata
    27661 tcagattgaa attaggaaaa ctttttttgt atgaagtggt aactaatccc aatttaaatg
    27721 tagaggtcat caataaaaat tctgcttatc agcaaatgta taaaaaaaaa tagtgactta
    27781 aatgtatata atagtataat cttcttattg attcaaaagt taaaactttt aatacaaaac
    27841 aatcattcta ttttaacatt tatcaatatg tctaaattta tataattaac acatacgaat
    27901 agtattgttt aaatcacatt atttaagata gataaatctt aaccctactc cagcataaat
    27961 actaaaatag ggcttagaat tatctatatt tttatcccat aaaatgctta gctcttcctc
    28021 ttgattgaac atatatatct ggggaaatac aatagaagcg taaatttata aaatttccaa
    28081 agtcagaaaa cttcagttca aacttaggta taccaaaact ttcaggtgct atttcgtcaa
    28141 cgccaaaata gatgataatt ccattatctg taagataaaa tgcttggtct tcaggtataa
    28201 ctaactcaac attagaataa tacatttctc tattttcatt tatttgagat ttaatgtaat
    28261 cagtaattat attaatataa tcaactccat ctctaaaaat gtcattaagt aaaagttctt
    28321 ttccagttgt caaatctata ttgtaattat tcaatgtatt gtatttaggc ccatagttat
    28381 tagcaaaccc cataaggttt aatatcatac tcagtacata atttttatta aaagtaactg
    28441 cgtaactggt agatacccaa tagctttgtt ttggaagtga attttgagaa gttgcgttat
    28501 tatattctcg ttcttcttcc tgtaagccat ttttaaaatt atttacagtc attcttacag
    28561 cattgtttat gttatttaaa ataactgggt ttacatacgt ggattgattt ctaatattgt
    28621 atataacagg atactttaca tcatatgaaa aatacttact tctttcaaaa tcttcattaa
    28681 attgtacata taatatatta tatgtacagg tatttgtaga ttgataattt tccattaaaa
    28741 tcctccccct tttataaaaa aatcagatac aatataatat gcttaaaaca gtaataatgt
    28801 taatggtaaa tgtaaagaat agaaagtatc taaaacagca aaaaatgact aattttgtgg
    28861 tataatacat aggattgaga gttattttat aggaggaaaa atatgttatt attagcagat
    28921 aaatggaaag attatgaact tatagatatg ggaaatggag aaaaattaga acgttgggga
    28981 agttatgtac ttagaagacc agacccacag gttgtatggc caatggaatc tgaatgggct
    29041 ttatggaaaa accctcatgg acattatcac agaagcaata aaggtggagg tcaatgggaa
    29101 cacaaaaaaa gatatccaga acaatggact ataaattata aaaacttaaa gtttcacatt
    29161 aaaccaactg gctttaaaca tactggatta ttcccagagc aagcagctaa ctgggattgg
    29221 atgatagaga aaataaagaa agctaacaga cctataaaag tgttgaatct ttttgcatat
    29281 acaggtggag ctactgtagc atgtgcatct gctggtgcag aagtatgtca tgttgatgct
    29341 gcaaaaggta tggttaattg ggcaaaagaa aatatagaat tatcaggtct taaaaatcaa
    29401 acagtaagat ttatagtgga tgatgttgta aagtttgttg aaagagaaat aagaagaggt
    29461 aaaaaatatg atgcaataat aatggaccct ccttcttatg gaagaggtcc taaaggagaa
    29521 gtatggcaaa tagaagaaaa gttatacagt tttgtagatt tatgtatggg agttttatca
    29581 gataaccctt tatttttctt aattaattct tatacaacag gattttctcc aatagttctt
    29641 gaaaatgtat tgtctacaac aataggtaaa aagacaaata caggagatat atacggtggg
    29701 gaattaggaa taccagcttc cagagatggt aagatacttc cttgtggtat atttggaaga
    29761 tgggagaaaa aataatgata aaagtcatat atgaagataa ccatttatta gttgtagaaa
    29821 aaccagttaa catattgtct caaggagata atactaatga taaagatatg gtaaatcttt
    29881 taaaagacta tattaaaaaa aagtataaca aacctggaaa tgtatatatt ggtttaatac
    29941 atagattgga tagaccagta ggtggagtta tggtgtttgc aaaaacatca aaagcagcat
    30001 ctagattgtc tgagcaggta agaagtaaga ctttcaaaaa aacttatcta gctgttgttc
    30061 atggcaagat aaaatctaac tcagatatac taaaagattt tttatataaa aataaaaaaa
    30121 ctaatatggt aactgtagtg aaaaaaagcc ataaagatgc caaagaagct gaacttaact
    30181 ataatgtatt agattttaaa gaaaatctaa gtttagttca agttaattta aagactggtc
    30241 gttctcatca aataagagtt caatttgcaa gcagaaaaca tccattatac ggagaccaaa
    30301 gatatggcaa agaaataaat aagattggac aacaaatatc tctttggtcc aataagatag
    30361 aaatttgcca tcctactaca aaagaaaaaa tggaatttac atgtcatcca cctgacaaat
    30421 atccatggaa tatattttaa atctcaaatg aggtgattat atgagtaaaa aattttcaat
    30481 aggagaaatg tctaaacttc ataaaataag catacagaca ctaaggtact atgaccaaat
    30541 agatttattt aaacctgttc atgttgacca agaaagcaat tacagatatt actcaattga
    30601 ccaattttca caattagata ttataaaata tttaaaatat ttaggtatgc ctttaaaaga
    30661 aattaagtca aagcttgatg aaaataatga agaaattcta aaattactaa caaataaaat
    30721 taatatagtg gatgaaaaaa ttaaagaatt agagcttata aagaaggtac taagcacaaa
    30781 gaaagaaact atggaaaaag gtatgaaagt agatgaagtt ggtaaaatag tgagaaaaca
    30841 tattccacga agaagtatct tatctataga gtatgaagaa ggattaagta gtgaagaaac
    30901 tatggaactt tccagaagac aaatagcaaa tatattagaa gataatatat ctgtgttcta
    30961 tggtggagta agtggacttg tatctataga taaattttta aaagaagata aagttattta
    31021 tgacaatagt tttgttactg ttgaaagaga cttatttgat ataaaatcac aaaaacatat
    31081 aacagaaatt tcatctggtg attttatttg tatgacatat aaagggcctt acaaagaaaa
    31141 ttataccgct tttaagaaac tggttgatta tgttgaatat aaaagaatac ctattgaaaa
    31201 ttatatatat gaaataccta taatagaccc tctgtctaca aacaacgaag aggaacttct
    31261 aacagaaatt caaatacaag ttaaaaagag agaaattgac ttaagatgtc tagtgtagta
    31321 atctagttta cttacacatc ttaatatagt tgtctattat caacatatga tttttctaga
    31381 tagtgattcc taacagtgag attttcaaaa aataggagga aatatggcta atgctaattc
    31441 cctcctattt ttaattaagc catatcattt taattaagcc atgtcatcac gaagtgcatc
    31501 aataatattt tcttttttta tcttattaat agtatacaac attgtaataa atactataaa
    31561 taaaacacta aatatactaa ttccaatact aaaccatgga atcacaaaat caatatcatc
    31621 tgtaaccatt actttatgaa ttagccatga agaaattact gctataggaa gtccaaagaa
    31681 caatgctctc atgccataaa aggcacactc aaaattcatc atcttttgga aatcatgctc
    31741 agacattcct acagagcgaa gcatggcaag ttctctacga cgtaacttaa tattagttga
    31801 aattgtatta aacacattgg caatcgaaat cagagaaatc ataattatga aagtataagc
    31861 aaaaacattg gcaataaaaa tgtaattacg gttctcatcc atcattccat acatattgta
    31921 cagattgtac tcattcgtaa ctccctcact ctcaataatt tgctccattt ctgcaactga
    31981 ctgagtagga tttttcgatt gaaagcttaa actttcaatt cctgtagaaa caccaggaat
    32041 ctcaaactta tctttggctg aataaggagc caatactctg aagcttgctt tcttcacatc
    32101 tgaacttctg attcctggag gagaatcagg tggcactact ttgacaaatg aaatattcac
    32161 attttgtctc tcaggtatct ccaacttgcc attagtttca ggagtgaggc taacattcat
    32221 agaagaactg ttgaaaatat caataatatt attatccttt gttaccatag aaacagcaat
    32281 cagttttgca ttttgtccag tatattcttc tgctggtaat ccaagacttt taataatatt
    32341 cagataagtt ctatcatcaa gaaattgcat atctactggt aattcaagca tttcactcat
    32401 agaatgtgaa ccctcatagt tcaaataatc gtctgtaagc tcacttactt tggcaacaca
    32461 tgaataggtc ataagtttct gatacaaacc ttcgtaaaca ccatttgcag tttttagctt
    32521 atcgtaaagc ttcaacattt cgttgtcttt cataccttgt gtagaaaaaa taatatcctg
    32581 gtcagtaacc tctgacctct gatttgacat ctgttttagg tctgctacta aagcacttgt
    32641 agatataaat agaactatgc ttaaaacaag cgacaataca atacttcgat aacgtttctt
    32701 gtttctttta aagtttttca tagcaagaac tccctcaaga ccataaactc gctgtgttag
    32761 ttttgatgtc ttcacagatt tagattcaat ttttacctca tttgtctgac gaatacactc
    32821 catcactggt gtgttggcag cctttcttgc tggtatgtaa gcagaaatta agattgtaac
    32881 catacttacc actgctgcaa cgatgattgc agggatagac aatgttaatg tcaacggaac
    32941 gtttacatag agaatatttg caaagttttt agctatgaca gaaatcacaa gtccaatact
    33001 gactataccg attgcaacac caattggtat accaatcgca ccaatgcaaa ttccttcaaa
    33061 tagcaccgaa tttcgtaact gttttgctgt agctccaaca gatgaaagta ttccaaattg
    33121 atgtgttcgc tcgttcaatg acatgttaaa tgaattatat attagaaaaa ttgagcctag
    33181 catgattatg gcaagcacaa tgccaccaac cgagtacaaa aacatattga acaaattgtt
    33241 atcagaaaca cctatgaagc gcaacacatt gtcattgaaa atggaagcat ggtctttagt
    33301 gctacttatg taagagcgaa tctgatgtgg atttttcaat gtgacaaaaa ggctaagact
    33361 gtcagtcttg acttgtttat cttctttggt tatcaaggta tatcctggtg cagaagattc
    33421 ttcaaatacg ggctttctac agatacccac aactgtataa gttttcttat cctgtggcac
    33481 aaaaacttca ctcccagcag tgtatgggtc actctgacca agcttttcat tttcactcat
    33541 tcgtttccct acatccaaag aaatagtatc acctactgca aatttgacac caccatctgt
    33601 cgcaacttta cttgaaacaa gaatctctct actattttca ggtaatcttc cagaaattaa
    33661 agttataggc aaagtatcaa aagcttcttt gctaaatcca gcaataaaaa gatatggttt
    33721 atctggattt ctgttacctt caagtgttgc atagccaata ttttcaaatg ttgcagtatt
    33781 tgcaacttca tcattgactc tttgttcctg tacaaaagaa gaatccacat ccaaaaactg
    33841 gacatgccaa tcaccatatt ttataatagc tccatttgtc atatagttca tcaaagaaac
    33901 tccaaaggta gtaactgctg taatcatagc agctgacaga acaactccta taattgttac
    33961 aagagttcga gtgcgattct ttttcaatcc ttgcagagca actttgttaa aaatgttcat
    34021 taacgattca ccttcctctc atcacgcact acttttccat ctgaaataca gataattcta
    34081 tctgcctgta aagcaatgtt ttcatcatgt gtgacaataa tgagagtttg gtgatattta
    34141 ctatggctct ctttcagtaa ctggataatc tcttgtccat ttttactgtc taagctacct
    34201 gtaggctcat cagcaagcat aactgctggt gcattcatca atgcacgacc aatggcaaca
    34261 cgttgctgtt ggccaccaga aagttgattt ggtaaatgtg tttttcgttc ttttaaacca
    34321 agcaattcta acaagtcatt taaccgttct tgattgacct tacgcttatc cataagtata
    34381 ggcaatgtga tattttctac tacatttaat gtgggtatca gattgtgaaa ctgataaatc
    34441 aatccaactt gtcgcctacg aaaaatagca agtttttcat tgctttggcc atatacatct
    34501 tgcccctcca gatatatctt tccacttgtt ggtacgtcta ctcctgcaat ggaatgtagt
    34561 agtgtagatt taccagaacc agaagaacca atgattgcag taaactctcc tttttcaata
    34621 gtaagtgaga catggtcaag tgcagtaacc ttattttcat tcttaccata aaccttacat
    34681 aaatcttcaa tctttaaaaa cgccattata taagtctcct ttcatatttt ataaatatat
    34741 cataggactt tcaacttaca tttaagtgac atttaaatga gagattcgtc actttggaaa
    34801 acgaataaca aatattgcac caccttgtgg gtgattttgt gcagtaattg ttcctccttg
    34861 tctcataata atcgtcttac aaagtgccaa tcctattcca taaccggctg tgcttgaatt
    34921 tttcccacga taaaatctgt taaatagaca tggtaaatct tcttttttaa agcctgctcc
    34981 gttgtcatga atagtaagtt gagtaaacaa aaagttgtcc tcacaaatga tatcaatttt
    35041 tccattatca cccacactct ccatgcagtt tttaaatata ttttgaattg cttctgaaag
    35101 ccaatttaaa tcacccaaaa tccttactcc ttttggtata tcaatatgta aagtgatatt
    35161 gtgcagttcc atgggaatca aaagttgatg aagtgcagaa ctaatcaaat tattcacatc
    35221 aatttgttct ttttgaaaga ctacaatacc tgcatctaaa cgtgataatt ttaaaaggga
    35281 agtaatcagc cagtccattc gtataagtaa ttcctctgtt tctcgtatca actcttttct
    35341 ctcatcttca tcagaagtat tctctaacag tgacaaaata agagttactg atgtaagagg
    35401 agtgcgaagc tggtgggcta tgtcagctag tgagtcagca aggtgttctt tttctttttt
    35461 caatgcgtaa ttttgctctc taatacgtaa cgtcatttta gttatttcac tttgcagaat
    35521 agaaagttca ccctcatctg attcagcaat atacagatgg ttagcattat gaagcacaag
    35581 attaatttgt tctgaaatct gtgcaatact tttgtatcga tttttagtaa acacaaaaaa
    35641 tgctattcca aaggtagtgg cagaaacaat agaaagtatt ccagtcataa tactgattgc
    35701 aaatcccagg gctacagtga gaatagtaat taataagaat aaaatagcaa attttcgaaa
    35761 ctctctattt cgaaacataa cttcccccca atctataccc tgtcccacga acagtcaaaa
    35821 ttatttgtgg acttgaaggg tcatcttcta tcttctctcg caagcgtttg atgtacacag
    35881 tcaatgtgtt gtcattaaca aactcacctg ccgcatccca caattcatca agtaatctta
    35941 ccctcgtaat aatactttta ggattgctaa taaacaccaa taacaaacgg tattctaaag
    36001 ctgaaagaaa aacctcattg ccattctttt tcacaacacc ggttgccgta tctacataaa
    36061 gcccacaaat ttcaaaagtt gacccaaaac gcccactttt tcgtagtgct gttctaattc
    36121 gcgcaatcag ttcacgtgga cgaaaaggtt tagtaatata atcatctgca cccatattca
    36181 gtccagtaac aacacttgat tcatcaccgg aagctgtcaa aaagataaca ggaatgtctt
    36241 gcttttcttt gatttctgtg caaatcgtaa acccatttcc atcaggtaaa gaaatatcaa
    36301 taagtgccaa atcaaattta ttcttagcaa tctctacaag tgcttcactt cttgtggata
    36361 catgggttac tataaatcct tctgaattaa gcaaaagaat aagatttttg gcaattgctt
    36421 tatcatcctc aactaaaaat atccgtttca tttactttca tcctccttta ttttattagt
    36481 tttatatatt ccagcagagc aatagtacac tactatttta cttatcatta cacaattttc
    36541 agtagtttct tttgttctta tataatccat gcataatacc tccattgatt tattatattt
    36601 cttataacat aaatacacaa gataagacaa aaaacataat gtaagtgcaa gttttttaac
    36661 tcactaactt ataaacatac actcctacta acactacttt cttgatgaca catcttacaa
    36721 gcttatattc atacatcaat gaaaaattaa atttatccat ttattaaaat aaaaggcaat
    36781 agcttctata gctaatgccc tttatcttaa aatgatttaa atactataca atttttataa
    36841 atggctataa ataaactcgt atagaatata aaatttatct acagtctttt gttatacaaa
    36901 atttataacc tcttatagaa caaatttacc actttgcaaa cttatcactt ttactcagct
    36961 atattataaa cttttaattt atctttgttt aaatgtttct tcttaacttc tactaaagct
    37021 tttagcgtat ccaaagatgt cattatttca gtagaaaatt caatggctgt tcttctcatc
    37081 ttaaatccat ctcttgtaga gtcatttcct ttagttggtg tatttattac tatatcaact
    37141 tgtttatttc ttatagcatc taatatgttt ggtctaggct cttcaattct atttactata
    37201 tcagcctcta ttccattttc tcttaaactc tt
//
LOCUS       DAGSRM010000034        34910 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.034, whole genome shotgun sequence.
ACCESSION   DAGSRM010000034 DAGSRM010000000
VERSION     DAGSRM010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34910
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.034"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(175..831)
                     /locus_tag="MJE33_001825"
     CDS             complement(175..831)
                     /locus_tag="MJE33_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBY9647761.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGAAIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
     gene            complement(886..1515)
                     /locus_tag="MJE33_001826"
     CDS             complement(886..1515)
                     /locus_tag="MJE33_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBY9647762.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSEHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQVGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            complement(1646..2287)
                     /locus_tag="MJE33_001827"
     CDS             complement(1646..2287)
                     /locus_tag="MJE33_001827"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBY9647763.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTISVIAI
                     NGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            complement(2296..2967)
                     /locus_tag="MJE33_001828"
     CDS             complement(2296..2967)
                     /locus_tag="MJE33_001828"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBY9647764.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLPDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            complement(2972..3856)
                     /gene="rsgA"
                     /locus_tag="MJE33_001829"
     CDS             complement(2972..3856)
                     /gene="rsgA"
                     /locus_tag="MJE33_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBY9647765.1"
                     /translation="MLEGKIIKGISGFYYVDTYNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDENEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLALEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVTNGEISKERY
                     DSYIQLLHEIRQNNSRRY"
     gene            complement(3954..4691)
                     /locus_tag="MJE33_001830"
     CDS             complement(3954..4691)
                     /locus_tag="MJE33_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBY9647766.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTILNEKNFSVKLFKADISNREDVEDMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDALVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            complement(4799..6814)
                     /gene="pknB"
                     /locus_tag="MJE33_001831"
     CDS             complement(4799..6814)
                     /gene="pknB"
                     /locus_tag="MJE33_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBY9647767.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     DDEDEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLSQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPGSNSGNSGGNSGGSNSGNSGGNSGNNGSNSGGSNSGNSGGNSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGANIGKTE"
     gene            complement(6831..7583)
                     /locus_tag="MJE33_001832"
     CDS             complement(6831..7583)
                     /locus_tag="MJE33_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBY9647768.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            complement(7603..8634)
                     /gene="rlmN"
                     /locus_tag="MJE33_001833"
     CDS             complement(7603..8634)
                     /gene="rlmN"
                     /locus_tag="MJE33_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBY9647769.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGHIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            complement(8642..9967)
                     /gene="rsmB"
                     /locus_tag="MJE33_001834"
     CDS             complement(8642..9967)
                     /gene="rsmB"
                     /locus_tag="MJE33_001834"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBY9647770.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFVLDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLINSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQDVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            complement(10029..10730)
                     /locus_tag="MJE33_001835"
     CDS             complement(10029..10730)
                     /locus_tag="MJE33_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBY9647771.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            complement(10799..11551)
                     /locus_tag="MJE33_001836"
     CDS             complement(10799..11551)
                     /locus_tag="MJE33_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HBY9647772.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTAVTYRVIKGKYVNSNIMKINFNIVSGLFPIISFIASSFGMSKSDIRR
                     IYIKLNNEYIYSNKYNFNPEDIIILIPHCIQENSCKLKVTNDIDNCKECGRCNIGELI
                     KLKEKTNVKIFVATGGTLARKIIMDNKPKAVVAVACERDLTSGIQDIKKIPVLGVFNK
                     RPNGPCVNTNVDIMDIEKAIGFLTGKNILVCN"
     gene            complement(11561..12490)
                     /locus_tag="MJE33_001837"
     CDS             complement(11561..12490)
                     /locus_tag="MJE33_001837"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBY9647773.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTTYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            complement(12505..12945)
                     /gene="def"
                     /locus_tag="MJE33_001838"
     CDS             complement(12505..12945)
                     /gene="def"
                     /locus_tag="MJE33_001838"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBY9647774.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEELIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            complement(12963..15488)
                     /gene="priA"
                     /locus_tag="MJE33_001839"
     CDS             complement(12963..15488)
                     /gene="priA"
                     /locus_tag="MJE33_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBY9647775.1"
                     /translation="MNNILNLSRGFIMKKYAKVIVRSNTIYTDNLFTYQIPVFLSDVI
                     KIGHRILVPFGKGNKPTEAFVFQFTDSLDEKIKIKEIIDILDENPIFRKEDLELVYWM
                     KNRYLCTYIECINLIYPKGYKLNNYKVVLLGESLSGLNDIELKEKISTLSDKNREIVE
                     KVIDSKGKIKVDKLKYIPNLNSSLYTMNKNGIIKLCWEYKNHKNEKKVCYISLSLESD
                     KIDDYIEQNKINVGSKQKEILSFLKNNENVEINDLLDLLNASKQSINSLSKKKLITLE
                     FKDYYREPKSIYKSVLKSIKLNNEQQEAVDEIKSNMFVDDKKPYLIHGVTGSGKTEVY
                     MEIIEFALNQGLDSIFLVPEIALTPQTIDRLKSRFGDLVGVFHSKLSEGEKHDVYKAV
                     KAGKVRVLIGARSALFAPFNSLGLIIIDECHESSYKSEKNPKFNAIEVARFMALKNNI
                     TLILGSATPSIEEYYRAKSGEYKLINIKSRANDKPLPNIEVVDMKDELDKGNRSIFSL
                     KLQKEIRYAIEENNQVILFLNRRGYANFVSCRKCGYVFQCENCDISLTYHKKSNTGRC
                     HYCGYEKEIPKECPECKSTYVKPFGVGTQKIEEELKYIFPDIKTLRMDKDTTSKKGAL
                     DEILNKFKDKEADVLIGTQMLSKGLDFENVTLVGILSADMILNFPDFKSFETTFQLIT
                     QVSGRAGRADKEGKVVLQTYDTEHYAIKHAIEYDYEGFYEDEIKIRKAFGYSPFNNML
                     SVVVSGEDERLVIKNIKNMHASLIYLLEKRGINDLGFILGPNPCSISKINQNYRWQIL
                     FKDENIEINLLKGIIKYICITKRDLIFDKNINVSIDINPNSVL"
     gene            complement(15727..16926)
                     /gene="coaBC"
                     /locus_tag="MJE33_001840"
     CDS             complement(15727..16926)
                     /gene="coaBC"
                     /locus_tag="MJE33_001840"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HBY9647776.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLTFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GTGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILIGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
     gene            complement(16931..17197)
                     /locus_tag="MJE33_001841"
     CDS             complement(16931..17197)
                     /locus_tag="MJE33_001841"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBY9647777.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            complement(17200..17817)
                     /gene="gmk"
                     /locus_tag="MJE33_001842"
     CDS             complement(17200..17817)
                     /gene="gmk"
                     /locus_tag="MJE33_001842"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBY9647778.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLKELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            complement(17847..18728)
                     /locus_tag="MJE33_001843"
     CDS             complement(17847..18728)
                     /locus_tag="MJE33_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBY9647779.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            complement(18732..19562)
                     /locus_tag="MJE33_001844"
     CDS             complement(18732..19562)
                     /locus_tag="MJE33_001844"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBY9647780.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKVNLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDRELKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGILEV
                     "
     gene            complement(19849..20883)
                     /gene="ltaE"
                     /locus_tag="MJE33_001845"
     CDS             complement(19849..20883)
                     /gene="ltaE"
                     /locus_tag="MJE33_001845"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HBY9647781.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALTLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGNKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            21179..22954
                     /locus_tag="MJE33_001846"
     CDS             21179..22954
                     /locus_tag="MJE33_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HBY9647782.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDDISKDLFIKNLEELEGPIFKSIYSKFLGISPIIAKEICYRAG
                     VNQNAIIKDISDEQFDSLHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEVANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIILSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            complement(23169..24821)
                     /gene="abc-f"
                     /locus_tag="MJE33_001847"
     CDS             complement(23169..24821)
                     /gene="abc-f"
                     /locus_tag="MJE33_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HBY9647783.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTESYSYISQSENNIETCSHSKEKSLLNAPDKFEEHLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGVLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKVSKEMNIK
                     IQDGMEIISKNLVEVKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINNKVKVGYFDQNQSLLDEEKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYDEYTISRNKKTPSRENQIKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQINKLKNSF"
     gene            complement(25120..26331)
                     /locus_tag="MJE33_001848"
     CDS             complement(25120..26331)
                     /locus_tag="MJE33_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBY9647784.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKIVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIASITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLILRLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISIGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            complement(26615..27148)
                     /gene="pyrR"
                     /locus_tag="MJE33_001849"
     CDS             complement(26615..27148)
                     /gene="pyrR"
                     /locus_tag="MJE33_001849"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBY9647785.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            complement(27508..28455)
                     /locus_tag="MJE33_001850"
     CDS             complement(27508..28455)
                     /locus_tag="MJE33_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBY9647786.1"
                     /translation="MFYFLKVKVGDIMDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKVEKARYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKEDKITVDKPIGRNPKDRLKMAVVKD
                     GKNAITHFEVVERFDKFTHMRARLETGRTHQIRVHALSINHPLLGDYVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEVIKKIK"
     gene            complement(28424..28870)
                     /gene="lspA"
                     /locus_tag="MJE33_001851"
     CDS             complement(28424..28870)
                     /gene="lspA"
                     /locus_tag="MJE33_001851"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBY9647787.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLIDR
                     VRLGFVVDYFDFRIIWEYVFNIADVFVVVGTVFLCIYVLFFESKSR"
     gene            complement(29180..29959)
                     /locus_tag="MJE33_001852"
     CDS             complement(29180..29959)
                     /locus_tag="MJE33_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBY9647788.1"
                     /translation="MKKIIWGICICLLSIVGVYTYNHNTHNQDSSKEAYSLNDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQVLDILKKNDVKATFFVVGEKVEYNKEL
                     LKRQYDEGHEIGNHTFTHINVAKNSYSSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDAIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIQHLDKNKQVQK"
     gene            complement(30014..30496)
                     /locus_tag="MJE33_001853"
     CDS             complement(30014..30496)
                     /locus_tag="MJE33_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBY9647789.1"
                     /translation="MDTKKYKDKLLKERENLTNLVEDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDLALSKIENGTYGICDLCHNQI
                     DLERLDILPDTSLCNDCAKKENNLLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGILRD
                     "
     gene            complement(30762..32183)
                     /locus_tag="MJE33_001854"
     CDS             complement(30762..32183)
                     /locus_tag="MJE33_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBY9647790.1"
                     /translation="MVSFLGSIVVLIVGYFVYGTFVEKVFGTNDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGVIVAI
                     ILGLLFFKKAKHIKEQNNHKLAS"
     gene            complement(32329..33099)
                     /locus_tag="MJE33_001855"
     CDS             complement(32329..33099)
                     /locus_tag="MJE33_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY9647791.1"
                     /translation="MRAIIVEDEFPARKELRYFIENKSGIEVVSEFTNGIEVLDFIQE
                     NKIDVIFLDINIPHLDGMLLAKTLNQFKSRPKIVFITAYESYAVDAFSLDVFDYILKP
                     YSEERIISMLNKLEKSEMSDIELFNVNSNLYKYKKEAVNQEIEEITHKISLWKGDKLV
                     VIDIDDIYYCEANERQTFIYTEKEKFILKEGISEVENLINDKTFFRTHRSYIVNLTKV
                     KEIIPWFNNTYILKLKNSDYEVTVSRSKVKEFRLLMHI"
     gene            complement(33104..34306)
                     /locus_tag="MJE33_001856"
     CDS             complement(33104..34306)
                     /locus_tag="MJE33_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBY9647792.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIGGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRIDNGT
                     LIVFYVKE"
BASE COUNT        11131 a         6386 c         3499 g        13894 t
ORIGIN      
        1 atttaaatat atttaaaatt aatatacaaa aaaagtagtt cttattgtat taattaaatc
       61 aatgatttga aatataaaat taatatcaat aaaacctact ttttaatttt ttaaatattt
      121 atttttaatt tattttgttt ttaatttact tcgattttaa aatgtccttg acttttattt
      181 tataaatgta catatttctt ctactggttt tttagatgga ctcttcaaat tgtcctctgg
      241 cactccaaga gctaacattg cacctaaaaa atactctccc ttttcaaaac cagtttctgc
      301 ttctaaaaca gcttctatct catctgctgc atagttttga cttgtcaacc aacaagaacc
      361 atatccaagt tcaattgctg atagtgtaaa gttttcaatt gcagcaccta gactctgcat
      421 acctggattt cttataaata atttatctat agtctcctta ggtgcatcta taagctctaa
      481 ttcataatat cctgatggat tatatacctt tgtaaaaact aatactaaaa ctggagcttt
      541 taagtaaaat agtgtaaaat ttttaacaaa ttttctaaat ctattagcct tatccactga
      601 tactttatcc atttctacaa gtatttcttg ttgcttttta gttatcacat ctgctatctt
      661 ttccattaaa tcacggcgtt ttataacaac aaaatgccag ttttgaatat tcttgcctga
      721 tggtgctgca cctgctgctt ttatcatttt taaaatatct tcatctgata catcttgatt
      781 tttaaacttt cttacacttt gtcttttaaa aatagtatct tgtaattcca tttttaaatc
      841 cccccataac ttttttatta ttttaatata atttttaaac tcatcttatt ttaatatatc
      901 atgttctaac aaatctttaa atgaatctat aaaataatca tatacatcta cattcccaaa
      961 cccatatttg gcatatataa aaggtatacc tacaaactta gcactatttg catcaccttg
     1021 cgtatcacca acatagattg gatttattaa tttatttctt tcaattataa gtttattatt
     1081 ttctccttta tgcaaaccag tattaccagg acattcaaaa tctataaaat actgttctag
     1141 tttatgtgct tttaaaaaag attctatata tccaacttga caattactta ctataaataa
     1201 tttatatttt ttagatagta atttaagagt atcttctata tcatcaaata atttagctcc
     1261 attttcagat aaatagtcac attctttcaa agaacattca ttcaataaat tcattctttc
     1321 ttcttcactt aaatttggaa ataaacgatt tgctatttcc tcaaaagaaa gccccataag
     1381 acttctaaat aaatcttttg tgacacttaa ttctatttcc ttatgctcac ttaaaacatc
     1441 ctgccagact ttgcatactt cttcagttga atcccacaat gttccatcta aatcaaaaat
     1501 aatactatct accacaaaaa atccccctta tgtcaaagtt tattatatat ttataattat
     1561 atactaaaaa gcagatacaa tcataaatat attttacttg attgtatctg ctctacaata
     1621 taaaaccaat tgtcaaaatc aaattttata aatttctaac tacaagtaca cagccttgct
     1681 ctactttaat cttacaagaa ttactcaaca taacatttga tattccaata ggtacagagt
     1741 atttcatata ataattatcg agagggtatt ctaagccagt aagtgttaca cccttagcat
     1801 caccatttat agctattaca gaaatagtgt cccctgggtt tccatacaat gaaatttcct
     1861 cattttcaac tatatacatc tcttctttat cagaaagtat cctagaatat ataccatctt
     1921 cttttaaata atataatagc tttatatttg caagagtgtg gtcaatcctt ccccctaaag
     1981 caccaaaaaa atcaatatga tttgctttaa gtgtcttagc taaaaaaaga caaagctctg
     2041 tatcagtctc atctttttta gatggaaatt tttcaaattt aacaccttta tttttataga
     2101 agtttatctt ttcttcttct actgaatcta aatctcctaa tatataatct ggcattattt
     2161 ccattttata agtgtgattt gcccctccat ctgcacatat tatgcagtca taacattcct
     2221 taattataat atctcttgta acttcgtaat cttcaatttc gccatttaaa acaatacaga
     2281 ttttcattat taaatttaaa ctaattcttc aagagaagca ttctttctaa ataaattaac
     2341 tgtttcttga atgtcatcac ttccaaatat tgcagaacct gctactatta cattagctcc
     2401 tgcttgtaca acttcggcta cgtttttagg gcttatacca ccatcaactt ctatatcaat
     2461 gtttaatcct tgactatcta taatagcttt taactcttta atcttatcta aaacacaagg
     2521 aataaatgat tgtcctccaa atccagggtt aacagacatt aataatacca tatctaaatc
     2581 aggtagtaca tgtttaatcg tatctactgg tgtagctgga ttaagtacta caccagcttt
     2641 tattccatgt gattttatat tttgtatagt tctatgtaga tgaacacaag cctcctggtg
     2701 aacagttatt atatcacatc ctgcttttac aaattcttca atatactggt ctggattttc
     2761 tatcataaga tgagcatcaa ataccatatt tatattttct ttctttaaac ttttgactac
     2821 aagtggtcct aatgtaatgt ttggtacaaa gtgtccatcc attacatcga tatgaagata
     2881 ttcacatcct gcactttcta cttttcttac atcttctaat aattttgcaa aatctgctga
     2941 taatattgat ggtgctaatt taatcatgtt tctaatacct cctactatta ttttgtctta
     3001 tttcatgtag cagctgtata taactatcat atctttcttt agatatttct ccatttgtaa
     3061 cagcttcttt tatagcacaa ttaggttcat tttcatgaat acactttgaa ccaaacttac
     3121 aatcattaaa tttatcaaat tctataaaat aatctttaag ttcaacttcc tctatatctt
     3181 caagggcaag ggaactaaaa cctggtgtat ctgcaaccat tccaccaaat tctaatttta
     3241 aaagttcagc gtgacgagta gtatgttttc ccctttttat tttatcactt acaactcctg
     3301 tttgaagttt aaaattttca tctatttcat ttaataagct tgattttcca actccagatg
     3361 gtcctgcaaa cactactaca ttttctttta attcttcttt tactttatct atatttaact
     3421 tagtaatatt gcttacagga atgactttat agccactaag ttcatatata tttttaacag
     3481 tttctaatgt atcattatca tctaaatctg cttttgtaag gattattaca gtttctaaat
     3541 tttctttttc tgccaaaact ataaatctat ctaaaagaga taagtttggt ttaggatttt
     3601 ttattgcgaa tactattaat gctttatcta catttgcaat aggaggtctt ataagttctg
     3661 tatctctact gtcgatttct tctaagatac cctttttttc attttcatca acaatgctta
     3721 ttttgactct atcaccaacc aaaggtgtta ttttctgctt tctaaatatc cctctagcct
     3781 tacattcata aataccatta taagtatcta cataataaaa tccactaatc ccctttataa
     3841 tttttccttc tagcatcaaa tgcctccctt tataaaattt atataaacac atactgttta
     3901 tgagtagtat atcgtgttat tctttcctat ttttatataa taccacattt tttttaaata
     3961 acaaatcctc cgtttggact aattacctga ccagtgataa aatttgattt gtctgatgcc
     4021 aagaaaatag cacaatttgc tatatcttct ggagttccta gcctcattag aggggtttct
     4081 tccacaagag cgtctatatc ttcctcatta tatccagata acatgtcagt attaatgaca
     4141 cctggagcta tgctatttac tctaatattt gatggtccta cttctttagc taatgccttt
     4201 gtcataccta ttatgcctgc ttttgttata gaataatgca cttcacaaga cgcacctgat
     4261 ataccccaga ttgaagatat attaattata tttccttttt tttctgatat catgtatttt
     4321 aaagcaactt gtgaacaata aaagcttcct tttaaattaa tattcatcat attatcccaa
     4381 tcttcatcag ttatatctgt aaacaactta tcttgactta caccagcatt gtttacaagt
     4441 acatccaatc ctccaaattc ttttatacaa taatctacca tatcctctac atcttctcta
     4501 tttgaaatat cagccttaaa aagttttaca gaaaaatttt tttcatttaa tatagtgtac
     4561 aactctttag cttcattttc tgacttatta aaatttacta atacattgta accttctttt
     4621 gcaaaagctt tagacatagc tttaccaatc cctctagctc ctcctgtaat taaaactgtt
     4681 ttcttcttca taaatacttt tctccttata ttataaccaa ttcagtattt tatacaaaac
     4741 aaactgccct ttaaagaaaa tattttctat aaaaggacag ctcttatttt attaatattt
     4801 attctgtttt accaatgttt gcgccagttc ctgatgaagt tcctccatta tctcctgagt
     4861 ttgagcctcc tgaactatct ccattgtctc ctgagtttga acctcctgaa cttcctccat
     4921 tgtctcctga gtttgagcct cctgaacttc ctccattatc tcctgagttt gagcctcctg
     4981 agtttccacc actatttcct gagtttgaac ctcctgagtt acttccatta ttgcctgagt
     5041 ttccaccact atttcctgaa tttgagcctc ctgaatttcc accactgttt cctgagtttg
     5101 aaccaggttc tgttggtgta ttattctctg gtgttgttgg ttttttatct ggtactgttg
     5161 gagtctgtgt atttgatttt tctgaacctt tgcttacata taggtttacc tcatcacctt
     5221 cctgagctct tgatgagcca gcacccggac tctgacttaa aactgtacct tctttatata
     5281 ttgagctgta ttcatactta acagttccca cttttaagtt attttcttca ataagttgtt
     5341 ttgcattact caaagtaagt cccactacat taggaacaga tgcctgactt gaacctttac
     5401 ttacgacaac atttatagta tcaccctttt tgacatttgt acttccacct tcaggtgtct
     5461 gtgatattat acaattttca tcaacttcac tattatactc ttcacttttt acactcaggt
     5521 ataatccttc tttttcaaca gcactttgag cttcttctaa tgtcatattt acaagattag
     5581 gaacagtcaa agatttattg ccaaaccctc cagcaaataa aaacttatat gctaagaata
     5641 cttgagcaca caatatcagt atcaaaacag ccgcaattac ttttaatctt cttctagatt
     5701 ttgggctttc ctgttttttc ttctttttct tagttctctt acttggagta ctcttaggtc
     5761 tctgattctt tttagctctc attatctctt cctcttcttc atcttcatca tcatcttctt
     5821 cataaaaatc atcatagtaa tcttcatcat catccaaatc agctacttca acaggcttaa
     5881 ccactttttc aggagctggt tttgcaagtg ttggatttac aactttatta atttcttttt
     5941 catcaatttt cttagttgca aaatcatcat attcttttat aaaatctaaa tcaatatttt
     6001 tttctatata ctctatatct tctatcaatt cttcagcagt ttgatatcta tctgcactag
     6061 atttttctgt aagctttttt attgtagttc ttacactttg aggaatccta actttttctt
     6121 ctgatgtaaa gtctatatca tcattaatgt gttgaagtgc tatagaaatt ggactatctc
     6181 ccctaaatgg aacttttcct atcaacattt catataaaac tatacctaga gaatacaggt
     6241 cagcattatt agttacaaat ttacctttag cttgctctgg tgaaaaataa tgcactgaac
     6301 ctattatact accaatatta gtcattgttg aatttgaaac agctttggct ataccaaaat
     6361 cagccacttt tactactctt ccttcatttg atatgagaat attatgaggt tttatatctc
     6421 tatgtataat cccctttttg tgcgctgcac taagtgccat agctatttgt tttgttatat
     6481 ctaatgcagt atattcatct aatatacctt catcttgtat aatctctttt agattcttcc
     6541 catctacaaa ctccattaca atgtagtgaa cttttccatc ttcaccaaca tcataaacat
     6601 ttactatatt tggatgtgaa agacttgcta ctgcttctgc ctctctttta aactttgtta
     6661 gaaattcatc atcatctaca aattcaggtc taagtacttt aagtgcaaca gtcctgttta
     6721 ataatctgtc tttagcctca tatacaaagg ccattcctcc atcaccaatc ttcctgatga
     6781 tttcatatcg atttcctaaa attgtatctc ccacaatatc accgtcctaa ttatactttt
     6841 atcactataa ccgaaacatt atctttacca gaaacatcat tggccatact tatcaattct
     6901 tcacttatat tactagttct ctcatattca taatctaata ttaaatctct tatatcttca
     6961 tcatcaataa acccagttaa tccatctgtc gcaagaagta ttatatcact ttttttgatt
     7021 tctttcttaa aaatatctac aacaaccata tcatctgttc caattgctct agttattcta
     7081 ttcctctgtg gatgtcttct tgcctcttct tctgtaatga catgggccat cataagttct
     7141 tcaaccacgg aatggtctat agttatttta tcaaattttt catctgttaa caaataacat
     7201 ctgctatctc caacatttgc cacatataag acattattat acactatagc cgtaactaat
     7261 gtagttccca ttccttcaaa ttctatatct tccatagatt ttttgtgcac aattgagttt
     7321 acattattat aagcctgttt taatatatca tctatatagt ctatttttac attatcatgt
     7381 tgtaataagt tttcctttag aaaatcaatt atattttcta ctgctaattt actggcgact
     7441 tcaccttttt tatgtccacc cattccatca gctatggcaa atattcctat tggaccatgt
     7501 tctgtatcta taacttctcc ctcacaatag tcttcattgt tttttcttat ttttcctata
     7561 tgggaggcac aactataaac catacgactt cctccttaca aattacttgt attttcttct
     7621 caattgccca caagctccac taatatcaga acccatagac atcctaactg tggcaggtat
     7681 attgttcttc tccaaactat ccctaaattt ataaataaat gctttatctg gtttttcgta
     7741 ttctctttct tcaactttat ttattggaat taaatttaca tgacatagca taccttttag
     7801 gagcttagct aatgcctttg cttctttttc tgagtcattt actcctttta taagagaata
     7861 ttcaaatgta acccttctat ttgtcttttt tatataatat ctacatgcat tcaatatttc
     7921 ttcaatagaa taagcatttg ctacaggcat aatttttctt cgttcttcat catatggtga
     7981 atgaagtgat aaagctaaat ttatagctat ttctaagtct gccaactcat acattttagg
     8041 tactattcca caagttgaaa gagttatatg tctataccct atattaagac catttttttc
     8101 gttaattatt tttaaaaatt gttttgtatt ttcaaagtta tcaagtggct caccactccc
     8161 catcaataca agatttgata ccctcttacc agtatcttct tgaattttaa taacttggtc
     8221 taaaatttcc catggctcta agtttctaat taatccatcc attgtagatg cacaaaaatt
     8281 acatcccatc ctacaaccta cttggttaga aacacagaca gtaactctac tatcataatc
     8341 catcatcaca gtttctatta tatttccatc atctaataaa aacaaatatt tttttgtatt
     8401 atctacctta gattctaact ttaattctat atctatatgc cctatacatg aaacctcttc
     8461 taatttattt ctcaaacttt ttggtatatt gttcatatca tcaaaagttt ttgcaccctt
     8521 atatatccaa gagaatattt gacttcctct aaagggcttt tcatctatag ttttcataaa
     8581 ctctttaagt tcatcttcag taaaattttt taatactatc tttttctttt ccattctctc
     8641 actaccttat tctctttaat tttgctatga aaaaaccatc tataccatga atattaggat
     8701 atatctttaa atatccttta tcttgattgt ctaagtctac atttactgta tcaattggaa
     8761 caaattcaaa gttgtgattt tcttcaacaa aagaagtaac tatatttata ttctccacat
     8821 cttgaattgt acatgtacta tatactaaag ttccaccaac tttgacatat ttagatgcat
     8881 tttctaaaat tttcttttgt atagaagtga tgtcttttaa ctcttcttct ttcttatact
     8941 taatttctgg tttacgtctt attataccaa aaccagagca aggaacatct gctaaaacat
     9001 aatcaaattt acttatacta ttttcatcaa tctcagaagc atcaaatctt tctacaaaca
     9061 catttttaag ccccaatcta tttacagtag agtttataag ctttagttta tgctcaaaaa
     9121 tatctctagc tactacttga ccagtattgt tcattaaagt agctaagtga gtagatttcc
     9181 ctccaggagc actacataca tccaaaacaa atgaaccttc ttttggatta attactttcc
     9241 ctactatcat agaacttata tcttgaattg taaataaacc agccttaaat aattcatttt
     9301 tttctatatt tttcattttt tcgactttaa tagcttcttc taccatagga acttttaaac
     9361 atacaatacc catttcactt aatttttcaa taagttcttc tctacttatt ttaagtgtat
     9421 tcgttcttat atagatacta ggcttttcat tatttgcttc caacaaatct tcagcaaatt
     9481 cttgtccaaa tttatctatc cagtttttta taatccaaga gttataggaa tactttatag
     9541 ataagtattt tacactatct tcctcagtta tgtccattat agtctcttta gctcttattt
     9601 catttctaag aatagcattt atgaaaccag aagatttttt atcatatttc ttagataatt
     9661 ttacagtttc attaactgct gcataatctg atatactatc taaaaataaa atctgatatg
     9721 ttcccattct taaaaagatt ttaacataag ttgacatttt ttttaccttt atttttgaaa
     9781 gtttattgat tatataatct aaataatatt tattttctat tactccataa attaactctg
     9841 ttgcaagtcc tctatccata tcacttattt ctaaattttt aaaatgctta tttattgcaa
     9901 tatttgaata attgtcattt ttttctatat cacataatac tttaaatcca acttctcttg
     9961 catccatata aatttctccc ttttattaaa cataaaaaag ggttgtttct aaacaaccca
    10021 ctttactttt agtcatttct tctttgagct attagtaaca atctaagtaa ttgaagtatt
    10081 gaaaccaatg ctgctgcaac atatgttaaa gctgctgctg aaagtactct tctactttgt
    10141 cttgactcac tttcatctat aatccctaaa ttcccaagtt gaacgattgc tctacttgaa
    10201 gcattaaatt ctacaggcaa tgttattatt tggaataaca ctgaagccga aaacaatagt
    10261 ataccaattt ttaaaaatgg accagccata ataaatccta aaaatattaa aaaccaagat
    10321 atacttgaag caaaattcac tactggtact aaactacttc ttatttgaag aggtgcataa
    10381 cctttagcat gttgtatggc atgaccacac tcatgtgcag ctactgcaac agaagttata
    10441 gatgttccat aatatacatc ttctgacaac cttacagcct ttcttcttgg gtcataatgg
    10501 tcacttagat gccctctaac catttctatc tttacatcat acaaaccatt tgaatcaagt
    10561 actcttcttg ctgtttgctc tccagtatat cctcgacgtg tatttactct taaatactta
    10621 tttgttgttg aactcacttt aaattgtgca tatatagtaa gtaatatagc tggaattaat
    10681 acaaccattg ttgggtcaaa tccccaaaaa ccgccatata cagggtacat aaataacact
    10741 ctcctaaaat atatttttat tatacatcct cttaatattc acttcactta aaaacagttt
    10801 aattacaaac taatatattt tttcctgtta aaaagcctat agctttttca atatccataa
    10861 tgtctacatt agtatttaca caaggtccat taggtctttt gttaaataca cctaaaacag
    10921 gaattttctt aatatcttgt attccagatg tcaaatctct ttcacatgca acagctacaa
    10981 cagctttagg tttattatcc attataattt ttctagctag ggttcctcca gtagctacaa
    11041 atatttttac attagttttt tcctttagct taattaattc tcctatgttg catcttccac
    11101 attctttaca attatctata tcattagtaa cttttaattt acaactattc tcttgaatac
    11161 aatgaggtat taaaattatt atatcctctg gattaaaatt atatttatta ctgtatatat
    11221 attcattatt taactttata tatattcttc taatatcact ttttgacata ccaaatgatg
    11281 atgcaatgaa agatataata ggaaataatc cactgacaat attaaaattt attttcatta
    11341 tatttgagtt aacatacttt cctttaatta ctctataagt aactgctgtt gaacatagta
    11401 ttataagtat tagtgatatt attataatat ttatcatcaa ggagaatatt tgagttaaac
    11461 ttattataaa aaaattaaca actcctatca aaattcctaa tattaaaatc aaaatcccaa
    11521 cacttgttaa atacttcttt atatctgtca tacttttcac ctaacccaat actaaaccag
    11581 tttctatagt attccctttt atatattcac caaccaacat tctcttttta ttcggcatct
    11641 gaatttcttc aattaaaagt acattatcta tagtacttac ttttatccca tctttgctta
    11701 catcaattat tgtacctaca tcttttgtgc ttttttcatc taacacttta gttttccaaa
    11761 ctttcattat gacatcatta taggtagtat aggcacttgg ccatggattt actcctctaa
    11821 ctaaattgtg tatttctctg gcactttttg aaaaatctat gttacctaat gatttattca
    11881 ttattggcgc ataggaaaat tcttcatgat tttgtacttc tcttggagca tttccttctt
    11941 caattaatct taatgtttct tttaaagttt ctgcgcctat attcatcatt ttatcatgaa
    12001 gttctcctgc tgttatattt tcatcaagat ttacttctgt ctttagtatc atatctccag
    12061 tatctagccc ttcatccata tacatagttg taacaccagt cttttcttca ccatttatta
    12121 ttacccaatt tataggtgcc gcacctctat attttggaag taaagaaaca tgaacattta
    12181 tacatccaag tttaggaatc tctaatattt cttttggaag tatctgtcca aaagctacaa
    12241 ctactattac atctggattt aaagatttaa ttttatctat aaattcttta tctctagcct
    12301 ttactggttg atatacaggt atattatttt caattgccag ttcttttact ggactcatac
    12361 ctagtttttt acctcttcct tttggtttat ctggttgagt tacaacaccc aatatttcat
    12421 acttctcatc tattattttt tgtaaacatg gaactgctat atcaggagtt cccataaata
    12481 ctattttcat caaatcaact cctactactt ttctattttg tcaacaaaca aaattccatc
    12541 taagtggtct atctcatgac aaaatgctct agctaaaagt tcttctccct ctaactctat
    12601 tgtttcacca tttctattta aagctctgac ctttacataa tttggtcttc taacatttcc
    12661 cgcttctcca acaacactta aacatccttc ctcatctatt tgctccccag atgtttctat
    12721 tatttcagga tttataagtt ctataagctc ttctccaata tctattacta ctactctttt
    12781 taatattcca acttgaggtg cagccagtcc aacaccatct gcatcataca tagtttctgc
    12841 catatcatct aataattgta ttatcttttc atcaattttt tcaacctttt ttgatttttt
    12901 tcttaatact ggttctccta tttgaactat ttgtcttaaa gccattataa ttcctcctaa
    12961 atttataata cactgtttgg attaatatct atagatacat tgatattctt atcaaatatc
    13021 aaatctcgtt ttgttataca tatatatttt attataccct ttaagagatt aatttcaata
    13081 ttttcatctt taaagagtat ttgccatcta taattttgat ttatttttga tattgaacat
    13141 ggatttggac ctaatataaa tcctaaatca tttatacccc ttttttctaa taaataaatc
    13201 aatgatgcat gcatattttt tatatttttt atcactagtc tttcatcttc accactaaca
    13261 actacactta acatattatt aaaaggagaa tatccaaatg cttttcttat ttttatctca
    13321 tcttcataaa aaccctcata atcatattca atagcatgct taatagcata gtgttcagtg
    13381 tcataagtct gaagtactac ttttccctct ttatcagctc tccctgctct tccagagact
    13441 tgagttatca attgaaatgt agtttcaaaa cttttaaagt ctggaaaatt taatatcatg
    13501 tctgcagaca aaatacctac taaagttaca ttctcaaaat ccagcccttt acttaacatc
    13561 tgtgttccaa taagtacatc tgcttcttta tctttaaatt tatttagtat ctcatctaaa
    13621 gcaccttttt ttgaagttgt atctttatcc attctcaaag tttttatatc tggaaaaata
    13681 tattttaatt cctcttcaat tttttgagtt ccaactccaa atggcttaac ataagtactt
    13741 ttacattcag gacactcttt tggaatttct ttctcatatc cacaatagtg gcatcttcca
    13801 gtattgcttt tcttatgata agttaaagat atatcacaat tttcacattg aaacacatat
    13861 ccacactttc tacaagatac aaaatttgca taacctcttc tgtttaaaaa tagtattact
    13921 tgattatttt cttcaatcgc ataacgtatt tctttttgta atttcaaact aaatatactt
    13981 ctatttcctt tatctaactc atctttcatg tctaccactt caatgtttgg aagaggctta
    14041 tcatttgctc tactttttat atttataagt ttgtattctc cagattttgc cctataatac
    14101 tcttcaattg agggcgttgc tgaacctaaa ataagagtta tattattttt taatgccata
    14161 aatctagcaa cttctatagc attaaatttt ggattcttct cagacttata tgatgactca
    14221 tgacattcat caattattat taagcctaag gaattaaaag gtgcaaataa agctgaccta
    14281 gctccaatta acactcttac tttacctgct ttgacagctt tgtaaacatc atgtttttct
    14341 ccttcagata gtttactgtg gaacactcca accaaatctc caaacctact ctttagccta
    14401 tctattgttt gaggagttaa tgctatctca ggtactaaga atatactgtc taagccttga
    14461 tttagagcaa attctattat ttccatgtaa acttcagttt ttccacttcc agttactcca
    14521 tgaattaagt atggtttttt atcatctaca aacatattcg actttatctc atcaactgct
    14581 tcttgttgct cattgtttaa ttttatactt tttagtacgg atttgtaaat actttttggc
    14641 tctctataat aatccttaaa ttctagggtt attagctttt tcttacttag agaatttata
    14701 ctttgttttg aagcatttaa taaatccaat aaatcattta tctctacatt ttcattattt
    14761 ttaagaaaac ttaatatctc tttttgttta cttcccacat ttattttatt ttgctctata
    14821 taatcgtcaa tcttatcact ttctaaactc aaagatatat aacatacctt cttttcattt
    14881 ttgtgatttt tatattccca acataatttt ataattccat tcttattcat tgtgtacaaa
    14941 gaactattta agtttggtat atattttaac ttatcaacct taattttacc tttactatca
    15001 atcactttct ctactatttc tctattttta tcacttaaag ttgatatttt ttccttcaat
    15061 tcaatatcat ttaatccact taaactttca cctaatagga ctactttata attatttaat
    15121 ttgtatccct ttggatatat caaatttata cattctatat aagtacacaa atatctattt
    15181 ttcatccaat aaaccaactc taaatcttct tttctaaata tcggattttc atctaatata
    15241 tcaataattt cttttatttt tattttttca tctaagctat ctgtaaattg aaatacaaag
    15301 gcttctgttg gtttattacc ttttccaaat ggtactaata ttctgtgccc aatttttatt
    15361 acatcagata aaaatacagg aatttgatat gtaaataaat tatctgtata tatagtatta
    15421 cttctaacta ttacctttgc atattttttc attataaaac ctctacttaa atttaatata
    15481 ttgttcatat tttaatatac tttacaagat attaaaatct cagaatttat aagcaatttc
    15541 aagttattaa tattaacttt attttaataa ttaactatta attatatttt atactttatg
    15601 acaaaataaa tgctgaaaac tacaatttaa ctgacatttg cgagttttta ctcgctaaaa
    15661 ttaaattgca gctttcagcc actttagctg tgataaaata cacaaatatc atatttaaaa
    15721 aaggtcttat ttctcaagca acgctttaac tttatctaaa ataacattgg ctacttcttc
    15781 ttttttcatc ttaggatact cagtaatatt tccctctaca tctattattt ttacaatatt
    15841 agtatcagct ctaaaaccag caccttcttt agttaaatca tttgccacaa taaaatctaa
    15901 atttttcttt tttatttttc ctttggcatg ttctataagg tcattagttt cagctgcaaa
    15961 tccaattagt atcttatctt cttttatttt tcctatttca taagcaatat cataatttct
    16021 acttagttct attgataaat catcattctt ctttttaatt tttttgtcag aataagtttt
    16081 tggtttataa tctgcaactg ctgcactttg aattactatt ttattttcac ttaaatttga
    16141 tactactgct tcatacaaat cttgtgctga ctctatttga acaaattttt taagattttg
    16201 tggaatagaa agatttgtag gacctgatat taatgttaca tcagcacctc tagctatagc
    16261 ttctcttgct atagaatatc ccattttacc agaagaccta ttagttaaat atcttactgg
    16321 gtctatactc tctacagttg gaccagctgt aactatcata ctttgacctt ttaaatcttg
    16381 ctctttagta agattagtca aaacttcctc tactattata tctggattag ctagtttacc
    16441 tgttccaata tctccacatg caagccttcc actttccggt tcaataaatt tatatccaaa
    16501 gctctttaaa gtttgtatat ttttttgaac aattggattt tcatacatat tagtattcat
    16561 agctggtgct ataagaacct gtgcttttgt tgccataaca gtagttgtca acatatcgtc
    16621 agctatacca tttgcaatct tgcctataac atttgcactt gctggcacta ttaagaataa
    16681 atctgctttt tttgctaatg atatatgttc aacttcccaa gtttttggtt cttcaaacat
    16741 atcttctact acataatttt gactcagcga ttgaaatgta agtggtttaa caaattctgt
    16801 tgctgatttt gtcattataa catggatatt tgcatttagt ttttttaact tacttacaac
    16861 atcacaagcc ttatatgcag ctataccgcc acttatgcca attacaacag ttttatcttt
    16921 tagcatgacc ttactcctct tctgaaattt gttggtgttg ctcagcatga tgtctagctt
    16981 cttctatctc tatttcctct tctgtcaata atctatatgt tatttttcca cttgcaactt
    17041 ctttagttgc tacacataca ggcttttcct tagttttatt tgaaacatat ggttcttctc
    17101 catcaattaa ttttcttgct ctctttgaaa cagtccctac taaatagtat ctgttgtcta
    17161 ttttttctag tacctcattg atagatggtt ttaacataat tataattcct ccttaaattt
    17221 ctctattata atatttttat atctgctaac tttattcttt tcagatgata ttataccttc
    17281 tatttcttta gcagatgttt ttacatcttc attaaatata aaataatcat aatccttaat
    17341 cattttaatt tcttcatatg cacagctaaa tctcttctct atttcagctt ctgtttctgt
    17401 acctcttcca acaattctat tttttaattc tttaagtgat ggtggtagta caaatataaa
    17461 tactccctct gggcaaactt cttttatttg ttttgctcct tgcatttcaa tctccagtaa
    17521 tacatcttgt cctttttcta aacattccat tattgccgct tttggagttc cataaaaatt
    17581 atcataaatt tgagcatact ccaaaaactc acctttttct atcatctctt caaacttttc
    17641 cttacttatg aagaaataat ttactccatc aacttctcca gttctgggtt ttcttgtagt
    17701 agctgataca gaaagtttta ttgtatcatt ttctttaagt agttctttgc aaatagtacc
    17761 ttttcctgca cctgatggtc ctgatacaac tagcaataat ccttttttca caaacattta
    17821 agcattcccc ttctaatatt gtattcttac tctatatttt gtatttgttc tcttattttt
    17881 tccaattcgc tttttacttc tactactaaa ttagttatat ttatatcaga tgacttcgaa
    17941 cctatagtgt tagtttctct attcatctct tgtattaaga aatctatttt tctacctata
    18001 gagtcgtctt taaatatagt atttttaagt tgccctatat gacttttaaa tctaactatt
    18061 tcttctgtta tactgctttt atctgcatat attgcaactt cttgtgctaa tctattctca
    18121 tctattatac ttgggtcatc cagtaattca cttattctca aattcaattt ttctctataa
    18181 tcttctacaa cactacttga atatttttca atctcttcta tatgattttt taataaatca
    18241 catctcatgg caatatcttc agctaatttt ttaccttctt cacttctcat ttctcttaat
    18301 ttgattaatg catcttcaac tgcttgattc aacattgacc acaataaatc ttcatcttct
    18361 tccttttctt ctattttcac aatatcagga aactttgcaa cattcataac gctaatatca
    18421 tctataatat caaatttatt ttttatttct tttagtatgt ctatatactg agatgctaat
    18481 ccctcatcaa aatttaagtt tacatcttct ttgcctaata agtcaaactt tatgtataaa
    18541 tctactcttc ctctttttac ataatttttt actaaatttc taactttatc ctctaaaaat
    18601 gaaatttttc ttggaagacg tatgtttata tctgaatact tatgatttat cgttttacac
    18661 tctactaaaa agtaataatt atcatccttg tattctcctc ttccaaaacc agtcatactt
    18721 atagccatta attaaacctc caaaatacct tcacatattt ttactgctgg acctgtcata
    18781 taagagaaat ctcctacatc tattttaact gttccacctt gagacgttac atttacacta
    18841 ctttctacca tattaagtaa attgcacact attgctgatg cagtcatacc agtaccacaa
    18901 cctaaagtat atccacaacc tctttcccaa gtataaacat gtatattata cttatcttct
    18961 acctttacaa aatttacatt cgttttttca ggaaatattt tattattttc aatctctttt
    19021 ccatatttac aaacatcatc tacatctaat tcatctacta taactactgt atgaggaacc
    19081 cccatcaaca tagaagtaac tttaagctcc ctatctaaaa cctttatact ttcttgcaca
    19141 aaacgttctt tatttgtaag tacaggaacg tccttagcta taaaacttcc ttttcccata
    19201 ttgactctta tagagtttat ttctctatta gtaagattta cttttatatc ttttatacca
    19261 tctaatgtat ctactgtaaa actttctttt ttaactatac aattatcata aacaaattta
    19321 caaaaacatc taaggccatt tccacacata gctgctcttg aaccatcaga attataatat
    19381 accatttcta catctgcaac ttttgaatct ttcacaacta acaatccatc cgctcctaca
    19441 gaaaatcttc tatggcatac tttttttgct aaagcagtat aatcagatgg atttatctgg
    19501 tcgaatcttc catctattgc tataaaatca tttcctattc catgtagttt ccaaaatttc
    19561 ataagtatcg ctccttcgta aattaacact taaactatta taacataaaa aaattcccat
    19621 aaacttataa tttatgggaa aagttttgta atgacaacta taatttgagg atgtgaggga
    19681 gagttgccat tacaattagg gtaattatat tatgttttta agctagttct ttctatgttt
    19741 atatattaat atttctatat gaattttatg tatgttttat ttaaactgtc tatgaatttt
    19801 ttaattaaac atctggtaac tagctataat ccttctagta gatatctatt aacatacacc
    19861 tattaattct ttaagtgttt ttaaaacata gtctaaatct tccttagtaa tatctacatg
    19921 agttacaaat ctatattcac cattttccat tttattaatt tttatatttc tttcaaaaaa
    19981 ttcttttatt aaaacatcct cttttataat atcttcactc atcttaaaaa ataccatact
    20041 tatatctgga ttaacattgt ttacttcaat gccatcaatt ttatttaatt cacaagctaa
    20101 atattttgca ttttcatggt cttcattcaa tcttaaagtc attttttcaa tggcaattat
    20161 accagctgct gcaactatac ctacttgtct cattcctcca cccataactt tttgctttct
    20221 tctagctttt gctataaatt ctttatttcc agcaagtata gaacccattg gtgcagctaa
    20281 accttttgac acacagaaca taacactatc acaacacttt gctatttctt tagcttctac
    20341 acccaaagtt aatgctgcgt taaataatct tgctccatct aaatgtactg gtaagttatg
    20401 tttctcagct atttttttaa tttctcttaa attatcaaga ctcacaactg ttccacctga
    20461 atatgcattt tctacacata taagccctgt ctgtggttca tgtatgtcat ctcttctaat
    20521 tgccttttca acaagttcag gatttagagc tccactttcg tcttcatcta tagttctaag
    20581 ctgaactcca gctattacag caggtgcccc aacctcatga gtcacaatgt gataaccttt
    20641 accaataatt atttcttgcc ctcttaggca atgagtaaat agagctaatt gattcccaaa
    20701 agttccactt ggaaggaaaa tagcatcttc ttttccaact ttagctgctg ctaattcttc
    20761 aagtttattt acagtagggt catctctaaa gattgaatct ccaacaggag cctttgccat
    20821 agcttctctc atttcgtcag taggcatagt aacagtatca cttcttaaat cgataaatct
    20881 cattttttca accttctttc atatttatat actatagaac cttaaaattg attttttctg
    20941 taaagttcat atactttcat agcttatact ctacacaata attatactat atttacttta
    21001 tatttaatct actttaaatt attaatttta aaaataaagt ttcctctcta ataaatataa
    21061 ataattatac aatttaaata ttaatctcta ttgtttaaat tccccctagt cacaaagcgt
    21121 atattatgtg tataattaat ttactgactc attttggtca aagaaagggg tgctataaat
    21181 ggcattagat ggattagtta tacattcaat agtcgatgaa ttatcatcaa aacttacagg
    21241 tggaaaaata gataagatac atcaacctga agatgatgag gtaattttta atataagaaa
    21301 taataaagaa aattttagat tggttttaag tgcaagtgcc tcaaatccta gagtttatct
    21361 tacaagtaat tatcaaaaag aaaaccctct aaaagcgcct atgttttgta tgttacttag
    21421 aaaatacata caaggtggaa atatagtaga aatttctcaa ataggttttg aaagaataat
    21481 aaaaataagt gttgaatctt tagatgaatt aaaagaaaag acagttaaaa atattatgat
    21541 tgaaattatg ggaagacata gcaacataat aataactcat ggtgaagaaa ataaaattat
    21601 agattctata aaaagagtcc cttttagcat aagtagagtt cgtcaagttt tacctggtca
    21661 tgattattca ttgccaccag aacaaaacaa actcaaccct cttgatgata tatctaagga
    21721 tttgtttatt aaaaacctag aggaattgga aggacctatc tttaagtcta tatattctaa
    21781 atttctaggt ataagcccta ttatagctaa agaaatttgc tacagagcag gtgtaaatca
    21841 aaatgctatc ataaaggata tatctgatga acaatttgat tctttacaca aagtattttg
    21901 taatttattc aatgatataa attctaacaa atactctcct tgcattatta tagataaaaa
    21961 ggtggataag gtagttgatt tcagttgtat aaaccttaca ttatttagcg atttatcata
    22021 tataaataag gatagtatgt ctcgtattct ggaagatttc tatagaacta aggatattaa
    22081 agatagaatt aatcaacgtt catctgattt aaagaagagt atatctgtta agcttgatag
    22141 actttataat aaacttaaaa aacaagaaga agaattatca gaatccgaaa atgcagacat
    22201 ttataagata aaaggagaat tgattacttc atatatttat atggtagaaa aaggtatgga
    22261 aagtattgaa gttgctaact tctatgatga aaattgtaat gatgtaacaa tagaattaaa
    22321 taaaaacttg acaccatctg aaaatgctca aaaatacttt aaaaagtata acaaaatgaa
    22381 acatgctaaa gtcgagatat ctcatcaaat ttccttaaat aaggaagaaa tagattactt
    22441 agaaaacata attttaagta ttgaaaactg tgaaaattta gctgaattac aagatataaa
    22501 agaagaactt gctaaggttg gatacatcaa gactcaaaag aaaaacagta aaaaggatac
    22561 tatcccatct acaaaacctc atgaatttgt ttcttctgat ggatttaaaa tattggttgg
    22621 taaaaacaat aagcaaaatg actatcttac actaagactt gctgataatg atgacttatg
    22681 gatgcataca aagaatattc ctggttctca tgtaataata aagtgtgctg gtaaggaagt
    22741 ccctgataat actgtatttg aaggagctat gctagcagca ttctttagta agtcaaaact
    22801 ttcttctcag gtgcctgtag actatactaa gagaaaaaat gtgaaaaaac ctagtggttc
    22861 taaaccaggt atggttattt atgaaactaa cagtactatt tatgttactc ctgaagaaga
    22921 aacagtagcc aagcttaagg ttaaatctga ataaattctg cactaaatac aaaataaatg
    22981 ttagacaact acacattatc attctaaata aaaatagtgg ccttcttata aagtacaata
    23041 ataaggccac tttctaatta gcttactttt aattttctaa caaaatactt ttctgtttta
    23101 ataactatac ttatattccc aacaaatatt ttcagtcttc ttatatagat gtaattaaaa
    23161 agttaacact aaaagctatt ttttaattta ttgatttgtt ttagtaattc attatattct
    23221 gattcataca attctttttt tagattatct ttttctatag ataacataga tataacagtt
    23281 gtaagtctat tttctaatac tagaagattt tcttttttga tttggttttc tctactagga
    23341 gtttttttat ttctgctaat tgtatattcg tcataattac aatcaaactc ccttatttta
    23401 gcatctttta tctctataat atagttacaa atatgtgata caaatactct atcatgagac
    23461 actataagca tagttttatc agtatttatc agtgcctttt ccagtgcttc catagatttt
    23521 atatctaagt aatttgttgg ttcatcaaat actaaaaaat tattatcttc caatattatt
    23581 ttacatagtg ctatttttac cttttctcca ccactaagta cttttacttt tttgtagaca
    23641 tcatcacctt taaacccaaa taggcttaag tttatcctta taaaagattc atcaaatgaa
    23701 ctattaactt tagtattgta tagaacactc ttttcctcat ctaataaact ttgattttgg
    23761 tcaaaataac ctacttttac cttgttattt atttttatat tgtcattttt atcagccaat
    23821 atttctttta tcagagtact ttttccacac ccattatcac ctaataatgc tatcttttta
    23881 cctcttttta tcttaaagga aacattatct aacaggagct tattttctaa ctttagagtc
    23941 atatctttta cctctactag atttttacta attatctcca taccatcttg aatcttaatg
    24001 ttcatttctt tagaaacttt aggtttattc tttacctcaa gtttatcaat tctgcttttt
    24061 atagccttca catttgagtc caactttttc ttattagtct gaccacccat tttgtgcaac
    24121 ctagcttctg aattacccat tctttttggt gtttttctta tgccatcact taaagctttt
    24181 ttatataaaa gagctttttc aagccttttt ttctctttaa catattcttg atactcaaaa
    24241 tcagctcttt ttacttcttc atctttttgc attaaataat cagtgtaatt gcctttgtaa
    24301 gcttttagtt ttccatcttc tagttctaat atagttgtac ataactcatc cagaaaacgt
    24361 ctatcatgag atattaataa taatgctccc tcatgccttt taaacatatc ttccaaaacc
    24421 ccaatgcttt ttttatctaa gtttgaagtt ggctcatcag ctattatgat atttttctta
    24481 ttactcagcg catctgctat ttttaactta accttttccc ctcctgataa gtgttcttca
    24541 aatttatctg gagcatttaa aagactcttc tccttactat gactacatgt ttcaatatta
    24601 ttttcactct gacttatata agaatagctt tctgtaaggt aagtatatcc ttcatcaatt
    24661 tctatttctc ccaatattgc ttttaataat gttgtttttc cttgaccatt tgaaccaact
    24721 agacctattt tgtcattttc taatatttca agtttatcta tatccaaaat taatttatct
    24781 gcataatatt tctttaaatt ctctactttt actaacaaca taaaaaatcc ctccttaaca
    24841 cacttaaata taaggagaga ttctataaat aggcataaca aataaacgta tagactataa
    24901 tctactctcc ttaatttaag cattgcaaat aaagccacca aaataggctt ttcaaaaatt
    24961 acaatacttt aaattataaa aaatagatta tattctcatt gtcctagtta ccttgccctt
    25021 tctttcattt atttttaatc attatatcaa cattaattac aaatgtctat agaaaattaa
    25081 ctaattttag ttattcaata tacaacttat tctttaaatt tattcttctt caactttaaa
    25141 gtcctgatta tttaaaattc tgtttaatac aattccaaca attgcagcta aactaagacc
    25201 agttatcttt actgtacttg ttatagggat tccaatagaa atacctttat tagataaata
    25261 tgtggttcca agacctatta ataatataac tgctatgatt aatatattta ttttactttc
    25321 tacacaagaa ctgtccctta tagtttttaa tcctacataa gttatcatcg aaaatagcat
    25381 tatactaatt ccacccataa ctgaacctgg tatactttga agaaatccac caaactttcc
    25441 tacacatgca agcaatatag caaatattgc tgtacgtcta agtattgaag ggtcatagtt
    25501 tttagttata gcaagtactg ctgtattttc tccataagtt gtatttgctg gtcctcctaa
    25561 acaccctgca actatagtcg caaatccatc cccaagtaat gttctattta atccgggatt
    25621 ttctataaag tttttaccta ctacagcacc attagtagtc atatctccta tatgttccat
    25681 aaatacagct aatacaactg gagatataat cacaattgct cctaaactga acttaggaac
    25741 tgtaaagtta ggtattgcaa ataaacttgc ttctgtaata gtagttatat ctactaatct
    25801 taatatcaaa cagattatat acccagaaaa aactgctatt aatattccta attgttttat
    25861 aaatcctcta ccaaacttat tggttaaaat tgctattgct aaagttatag atgctattac
    25921 aaaattggct gaagccatac taaaagctgt tggcaataaa tttaatccta taactactat
    25981 catagctcct gtaacttgtg caggaaaata taatttaatt ttatttacac caactatttt
    26041 tactataatc gacattataa tataaattaa tcctgctact attataccac cttgagcata
    26101 tgctaaatct ccaccatatg cctctttagc tgctagtata actggtataa acgcaaaact
    26161 tgaacctaaa aaggcaggta cttttccttc tgtacaaaaa tgaaatatca atgtaccaac
    26221 accagcacag aaaattgcaa ctgacggatt aaaacctgtc aaaatgggaa ctaatactgt
    26281 cgctccaaac atggctaata gatgttgtaa agacattatc gtttttttca taaaaacctc
    26341 caaaattatt atatataaat aaatcctctg tttaattaat atcttttaat taattatcat
    26401 tccctagatt ctatttttta acctctatat acttattata taatttatca ttattttaca
    26461 ttttacgaca tcaaaaactc aaataaagga gaataaaaac tacattaaga ctcacatcca
    26521 taaaattata atatttattt tgtgcaatct atgtaagctt tatcctcctc cctgactatt
    26581 caatgctaag cttttatata atacttaaaa ataattattc tttgattgta actgaatctt
    26641 ctccatcaat ttctaataaa ctgacagata taatttcatg tctagaagta ggtacatttt
    26701 ttcctacata atctgctctt ataggcaact ctctatgccc tctgtccact agcactgcta
    26761 attgtattga tttaggtctt cctatatcta taatagcatc tagtgaagct ctcacagttc
    26821 ttccagtgta taaaacatca tcaactaaaa ctactgtctt atcatttaca ttaaaatcta
    26881 aataagttcc tttaactaca ggttcaacat gaattttttc caaatcatct ctatacaaag
    26941 ttatatcaat atctcctgta tctacttttg ttccttctat ttgttctatt ttcttagaaa
    27001 ttctatttgc aattggcaca cctctagtct ttattccaac taaaactaga ttttctacac
    27061 ctttatttct ttcaattatt tcatgactta ttctagtaat tgctcttgct attgcctttt
    27121 catccattaa ttgagctttt tctaccatat cataattatt gtcaaagata taatatatct
    27181 atttgacaaa tctactcctt tcttttaatt ttttataaat ttatttttat ttaaaatttc
    27241 aatttaaaca acaaaaaaac ttctcatcac aagacaagaa gctcttatat aaaaaatata
    27301 cagtgcaatt tagcaattgt cttacgatat ctctgtatcg tttaaagata attttttaat
    27361 ctatcaaatc aaattatata tttatatttc aaatttttca agctttttta aacaaaatac
    27421 tttaatataa gatattttat ttaaatttca ctaaatttat ttacttatat aaaaacaaaa
    27481 aatgatttat aagtattata caaatggtta ttttatcttt ttaataactt ccttaaaata
    27541 ctcaggtaat tctgaattaa attcgatata ttccttagta gtaggatgta taaatcccaa
    27601 cttcttagcg tgcaaagttt gtccttttaa tttaaattta gtttcttttg gaccatatac
    27661 ataatcacct aaaagtgggt gatttataga caaagcatga actcttattt gatgagttct
    27721 accagtttca agcctagctc tcatatgtgt aaacttatca aatctttcta caacttcaaa
    27781 gtgagtgatt gcattttttc catcttttac tacagccatt tttaatctat ccttaggatt
    27841 tctccctata ggtttatcaa cagttatttt atcttcttta accacaccat agcatataaa
    27901 ttcatactcc ctagttatag tatgttcctt taactgttct gctagaaaat tatgtgcata
    27961 attgttctta gctatcataa gaagaccaga agtatctttg tctattctat gaactatacc
    28021 aggtctaata actccattta ttgatgacaa cttatcttta caatgatata aaatcccgtt
    28081 tactaacgta ttttcatagt ttccaggagc aggatggaca accatatcct gaggtttatt
    28141 tattattaag acatcatcat cttcatatac aatatctatt ggaatatctt gaggtaaaac
    28201 ttctaatact ttaggttctg gtatctctat aacaatatta tcttcctctt taaccagata
    28261 tctagctttt tctactttat tattaacctt tacttttcca tcttttataa ttttttgtat
    28321 ataacttctt gacatatcac ctaattgttc tgctagataa atatcaagtc taacttcttc
    28381 ttcctcttca agtactaaaa actcttttac ttcatccatt atatcaccta cttttacttt
    28441 caaaaaataa aacatatata cataaaaaca cagttcctac aactacaaat acatcagcta
    28501 tattgaatac atattcccat ataattctaa agtcgaagta atctactaca aaacctaatc
    28561 gtactctatc aattagattt cccaatgcac cagatataat taatataatt cccaaccttc
    28621 caaatatatg tactttcctt gtattaagat agtatagtcc aaatactgtt gcaagtaatg
    28681 caacaattat aaatatccat tgattatttt gtaataatcc aaatgctgca cctctatttt
    28741 cgacataagt taaatgaaat acattattta ttattggtat tgttgataca tccaccaaat
    28801 tattcaatac ccatatttta gacagttggt ctaaacctat gagtagaatt attattaata
    28861 tatatagcaa tgatttttcc ccctataaat tatttaaaaa ccaattttat ttgaagcaca
    28921 ctattattat tatacaaaaa aaatgctcta aagagcaact ttattaaaat tgtattttct
    28981 taataaagct agaacaaaat ttaaaatagt actacttttt ttatattgta gatttctaca
    29041 tatttaaaat catatcatta tgtataaaaa atttaaaaat accatactaa tcaatatttt
    29101 ttgtgtattt tatagattaa agattaatga tgtctgtaat ttaaacttac aatcaccact
    29161 aatcttcaaa caaatatttt tacttttgca cttgcttatt tttatctaaa tgttgtataa
    29221 gttcagatac tgttacgaat ttatatcctc tctcttttaa tttaggaatt actacttcta
    29281 aagcttgtat agtttgtgat ttatcatttc ttttagtgtt ataatcatgt aaaagtatta
    29341 tattaccatt ttggaccttt gtaaggatag tatttataat tgcatctact ccagggttag
    29401 accaatctct agggtctaag tttgaccaaa gaattatatt catgccttta gaaactataa
    29461 tatcacagac acttttactc atggctctat atggaggtct aaatatcttt ggttctactc
    29521 ctattacact ttttataata ttttgcgtat ctgttatttc tttttcaaca ctactataac
    29581 tattttttgc aacatttata tgtgtaaatg tatgattccc tatttcatgc ccctcatcat
    29641 attgtctttt taataattct ttgttatatt ctactttttc tccaactaca aaaaaagttg
    29701 cttttacatc atttttcttt aatatatcaa gtacttgtgg tgtaaaatcc tcatctggac
    29761 catcatcaaa agtcaatgct aaaatttttt catcctcatt tccttttttt attattacat
    29821 cttcataatc gtcttttata ttgtcattta aactatatgc ttcttttgaa ctatcttgat
    29881 tatgtgtatt atgattatat gtatatacac ctactataga taataaacat atacatatac
    29941 cccatattat tttcttcatg tcattacctc ttttctaatt cattctagca atatatttta
    30001 taatgtcagt tttttaatct ctcaatattc catttttatt taaatcagtt aaatttgtta
    30061 ggtcttctga ataaaagtta gaatcttcta catcagggtt ttgcattgca tcacctaata
    30121 aattattttc tttctttgca caatcattac ataaacttgt atctggcaat atatccaatc
    30181 tttctaaatc tatttgatta tgacataaat cacaaatccc atatgttcca ttctctattt
    30241 tagataatgc taagtctatt tgatacaatc ttccctcttc atgatttatt agactgtttt
    30301 gcatattttg catatataaa tctgttccca tgtctcctat atgattatca taactagata
    30361 attctcctga tgaatacttt tcacttgtat gtttagttgt atctccaaat aaagtattat
    30421 ctttcatatc ctctactaaa ttagttaaat tttctctttc tttcaataat ttatctttat
    30481 attttttagt atccattttt aacacctcta attttaatat ttacatcata aaaatagtat
    30541 gtggaaaagt aaattaaaca tacaataaaa ataagatttg attcgaaaaa tggaatgtct
    30601 caaaatgaaa tttttagttc atttaaccct cttttttata tgaaatcaaa tatattttta
    30661 tcctttcagc aataaaaaag agacttatct ctattttgag atagtctctt ctttgaacat
    30721 aatttaaata caatttttta atttttatag ttattatttt actaacttgc caatttatga
    30781 ttattttgtt ctttaatatg ttttgctttt ttaaagaata aaagaccaag aattattgct
    30841 acaataacac ctattccatt agatatatta actggtagtc taaatccttc ttttgcttgc
    30901 attatataac taaatgttac tacagtcata aaaagtgctg gtaatgtagt tatataatgg
    30961 tttttatttg ctttgactaa atatgcagaa ccagtccaca acattatcat tgcaagtgtt
    31021 tgatttgccc aagagaaata tctccaaatt atattaaaat ctataaatgt aagagcaaca
    31081 ccaactgcga ataatggtaa agctattaaa aatctatttt tgactgcatc ttgattataa
    31141 ttcattgcat ctgctattgt cagcctagca cttctaaatg ctgtatctcc agaagttata
    31201 ggacatgcca caactccaag tatagctaaa gctcctccaa taggccccat taatccaaca
    31261 gatactgtat ttactactgt tgctgctcca ccatcattaa atatcacatt aagttgaggt
    31321 atgccatgga aatatgacat ggctgctgct gcccaaacta aagctattat accttctgca
    31381 accattgacc cataaaatac tggtcttgcc tcactctctt tctcaacaca tctcgccata
    31441 agtggtgatt gagttgcatg aaaacctgat attgctccac atgctattgt tataaataag
    31501 tatgggaata aaggtgttcc atttggattc atattttgta aatttatatt tggtatgtta
    31561 taccccttta ttattaatcc tccagctaca cctactgcca taattaataa tgctgctcca
    31621 aataaaggat atattttacc aatcaattta tctattggta aaacagtagc cataatataa
    31681 taaagtatta tcactccaac ccatatttgt gcgtttacac ctgtcaatgt ctttaataat
    31741 cctgctggac ctgttacaaa tacaactcca accaatacta aaagaataac actaaatatt
    31801 ctcattgcta tttttgcacc atttcctaga taaaggccta ccaactctga tgcagatgta
    31861 cctttgtttt tcattgatag atatcctgaa gcaaaatcgt gtactccacc agcaaagatt
    31921 gttccaaaca caatccataa aaatgctgat ggtccaaata atgctccttg tatagctcca
    31981 aatatcggac ctaaccctgc tatatttaga aactgaatta aaaagattct cttccatttc
    32041 attggcacat aatctactcc atccctacaa gctatagctg gagtttgatt tttgtcattg
    32101 gttccaaaaa ccttttctac aaaagtacca tagacaaaat aacctactat aagtactact
    32161 attgaaccta aaaagcttac cataaattca tccccttagt ttttatataa ttgctttata
    32221 aatttttatg agtttaagaa aactatttcc agttcaaata gtaatacttt tttatgctaa
    32281 tataaatatt aattacatta agtgcatatt taagtacatg aaatgacttt atatatgcat
    32341 taacagccta aattctttta ctttgcttct acttacagtt acttcataat cactattttt
    32401 taattttaat atgtatgtat tattaaacca tggtataatt tcttttactt tggttaagtt
    32461 tacgatataa gacctatgtg ttctaaaaaa tgttttatca tttataagat tttcgacttc
    32521 tgatatgcct tcttttaata taaacttttc tttctcagta tatataaatg tctgcctttc
    32581 attagcttca cagtaatata tatcatcaat atctattaca actaatttat cacctttcca
    32641 aaggctaatt ttatgtgtta tttcttctat ctcctgattt actgcttcct tcttatattt
    32701 gtataaatta ctatttacat taaatagttc aatatcactc atctcacttt tttctaattt
    32761 attcagcata gaaataattc tctcttcaga atatggtttc aatatataat caaatacatc
    32821 caaagaaaaa gcatctactg cataactttc atatgctgtt ataaaaacaa ttttaggtct
    32881 tgatttaaac tgatttaatg tttttgcaag tagcattcca tcaagatgag gtatatttat
    32941 atctaaaaaa attacatcta ttttattttc ctgaataaag tctaacacct ctataccatt
    33001 ggtaaattca cttactacct cgattccact tttattttca ataaaatatc tcaattcctt
    33061 tcttgctgga aattcatctt ccactattat agctctcata cttctactcc tttacataaa
    33121 atactatcaa agtcccatta tctattcttc ttatattaag ccctcttcca tacatcagtt
    33181 taagccttaa atgtacatta taaagcccaa tcttattttc tggcatatta tcttgatata
    33241 cattatttat aatttcctca cttataccta taccattatc ctctattgat attctcacag
    33301 tattttctaa tgaatacggt aactttttta tggatatttt cactacacca gctctaccac
    33361 tttctaaaat accatgtatt attgcatttt ccactaatgg ttgtataatc aaacttggaa
    33421 tctttatatt tacatcttct atatcataag atacagttag taattctcca aacctagctt
    33481 tttctatctc tacaaaagat ttaacttgtt caatctcttt atttatatca attaagttat
    33541 cactatattc aagattatat cttagataag ttgataaatt tattattaac tctctagcct
    33601 tatctggact aaatcttata aatgaagcta ttgtgtttag ggaattaaac aaaaaatgtg
    33661 gattaatttg tctttgaagt gcttttattt ctgctttatt tctcgcttct atatcttttt
    33721 tatctttctc aatcccctct actatagata taacaaatcc tattccaatc tgcccaacta
    33781 ttaaaggtaa atatattcca ccgattattt gaattgctaa agaatgtgga tatgatatta
    33841 agtatataag taatatagta aaactttcag aaatcatgcc taccaatatt ccatacatta
    33901 ctctatgttg ttttggtatc ctcttatata gaaatcctga taatacacct gctagtatac
    33961 ttgataaaag acatggaaca gatgttatcc ccccaatatc cataaaatat ctatgaatcc
    34021 ctgaaaaaac tcctgctgta atacctacca tcggaccaaa taaaatgcca cctgatacta
    34081 tagatattat tctagtgttt actattgacc ccatataatt tataccacta taagttccaa
    34141 atatggctaa aaaagtaaaa actaatgcta tacagaccaa gtcattaaaa ctatattctt
    34201 ccttttgaaa tatctgctta aatattttta actttgttat tagaagaaac aatactacaa
    34261 taaggcttgt cttttctatt aatgcgatta aaattgatgt tttcataatc cccctttaaa
    34321 aaataaattt ttacacactc taaatttatt atggttgtta taaattacat atttgaagta
    34381 atcctatcac aaaactttcc acatttaatc ttttaattaa ataagaaata gttgtacttc
    34441 tattaacaac taatactagc tagcataagc ttgtaataac ttgtagttat tattaacttt
    34501 acaattctag tatttaattt tatactttag aaatctcttt tcttagaaat aatactttcg
    34561 aatatcatat aatacaatat tatgtagatt cttactcata caacatattt ctttaaaaat
    34621 tttatactat tctttgttca aatatctcat ttgatttaat tgtgttaaac acacattatc
    34681 atctctctgt tatatattta ttatatagcc gtatatcgat aaagtaaata aatcccatat
    34741 ttaaaataat atgtttgaca ataaattatt ataatgtata tttaccataa taatttgtca
    34801 accttgaaag ttacttctat aattattgtt aaataattct tctaaaaaaa atacctataa
    34861 attttatttt ctgaaataca tagttataac tatttttatc acgtataaga
//
LOCUS       DAGSRM010000035        33670 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.035, whole genome shotgun sequence.
ACCESSION   DAGSRM010000035 DAGSRM010000000
VERSION     DAGSRM010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33670
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.035"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            704..1555
                     /locus_tag="MJE33_001857"
     CDS             704..1555
                     /locus_tag="MJE33_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBY9647793.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTIAIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVVGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKKTKEI
                     KSTEGEC"
     gene            1559..2029
                     /locus_tag="MJE33_001858"
     CDS             1559..2029
                     /locus_tag="MJE33_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647794.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            2042..3352
                     /locus_tag="MJE33_001859"
     CDS             2042..3352
                     /locus_tag="MJE33_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBY9647795.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYKNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKKGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHASDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGADVEITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIIPAK
                     AMALTVVDLLFDNAKDAKDILDTFTPVMTKKEYLTFLEKHDKTL"
     gene            3564..4310
                     /locus_tag="MJE33_001860"
     CDS             3564..4310
                     /locus_tag="MJE33_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBY9647796.1"
                     /translation="MFNAGNRIINTWLYPIQDGFVLIDTGYENGFKHFKKRISKFNIK
                     IKDIRYIFLTHAHDDHVGFLNQILDESPNTKIVMHDKALEILYKGQNSFIGGCTSRTA
                     FLFCQIMKLFGKGEHSFSPLKQEFEKRCILVSDKNRKEIEKELNGKIIDTPGHTDDSI
                     SLLIDDGVLFCGDSAMNGFPSTHKITIFAESKLEFIQSWKTIINTKPKMIYPGHGKPF
                     EYSKLEQNLIYVNKINLYPLHNSKDIHRFL"
     gene            4672..6213
                     /locus_tag="MJE33_001861"
     CDS             4672..6213
                     /locus_tag="MJE33_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBY9647797.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SLVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAELDVDGSLSDVEKKGLKKAGISILIYVAI
                     IVALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMVFNIPLGPGIL
                     PSYSM"
     gene            6248..7354
                     /locus_tag="MJE33_001862"
     CDS             6248..7354
                     /locus_tag="MJE33_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBY9647798.1"
                     /translation="MLDKNRLINNFMDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGEKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPILDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVITEKIPHRDIYLCFTICEEAGMHGVKNFNPDNLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFQGKKAHAGIEPEKGLNAILVASHAISNMHIGRI
                     DSLTTSNIGEIEGGGATNIVTDKVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHNMSYPSFELSRDSHVFKLSEEAIRQVGVVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVYHMIKL"
     gene            complement(7733..9019)
                     /locus_tag="MJE33_001863"
     CDS             complement(7733..9019)
                     /locus_tag="MJE33_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBY9647799.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDSDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFNLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRHSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAINENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
     gene            complement(9142..12240)
                     /locus_tag="MJE33_001864"
     CDS             complement(9142..12240)
                     /locus_tag="MJE33_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBY9647800.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKAMGLNDISHLK
                     CYKALQGKDSPCEFCTNSILKEDEYYIWENTNTRTNRHFILKDKLIKWEGKHARLEIA
                     LDITEKEDISKSIAEKLEIKELLVGCIKHLITESNFSDAVNLVLTNIGKFHKADRAYV
                     FEVSEDRKKVTNTYEWCNEGVDEQKQALTNLDINELKNWFLLFEQKGFVIIDDIENIK
                     EEQPIEYEILKPQKIESLMTSSLKQDDKVVGFIGVDNPHKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTGAYNRNKYIKYIDNFQKGKVKSIGVVFVDINGLKDIND
                     NFGHANGDSAIVSACVIIKKYFHSYNVYRIGGDEFVVLCEDISKQLFEELVSWLSEEF
                     KDEMIYSVAVGSIWSDDNIDIHKLIKVADERMYQDKKRYYNKNKLRNNEMKVISDLAD
                     STNNILFKHKELVDKVYFDDLMIKGYSKAYEKLAYNTYDIILSICIDTNLITPIYQSE
                     SNIFSFPIKENLEKTFSYIKKHLIHPDDIILFNDNHLLKSLASLNNSKQDDLELNFEY
                     RRLGIDKKYYWALIYMLKISSKNKLGEKQTHILVAIKNIDYLMQLHKNQKDTLTGLYN
                     YESFCEQISLMLKRYPDKRYAIIMMDIDKFKVINDIYGVRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDRDIHKVIYKIKNSLNRKKFEYMLVPCFGVYRILDNH
                     IPITNACEMAGYAHKKVKKQSINNYIFYDDTIRNDALEEKQMEREMEYALENGQFHVY
                     LQPKYNLNANKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIRLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYSIPKSFLELELTESLFIKN
                     IKLFTKIIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNEEGITDREK
                     IVLKHTIAMAKDLDMDVVAEGVETKEQAEFLVSLDCVVIQGYYYCKPIPSADFEKLLY
                     QK"
     gene            12966..13124
                     /locus_tag="MJE33_001865"
     CDS             12966..13124
                     /locus_tag="MJE33_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647801.1"
                     /translation="MKIFSFLLFVIGICLCIAGFIGHYPVFVGTTLSFLAIAMIAVRQ
                     VKTKKKMK"
     gene            complement(13411..14115)
                     /locus_tag="MJE33_001866"
     CDS             complement(13411..14115)
                     /locus_tag="MJE33_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY9647802.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKAQQDNINNDAPKSFEVKNLKIDYENHIVSINGEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(14106..15713)
                     /locus_tag="MJE33_001867"
     CDS             complement(14106..15713)
                     /locus_tag="MJE33_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HBY9647803.1"
                     /translation="MKKERLQHEDLKEYDYDEDGYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGVACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESN
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPMYDEEGKDKWK"
     gene            complement(15967..17145)
                     /locus_tag="MJE33_001868"
     CDS             complement(15967..17145)
                     /locus_tag="MJE33_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647804.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVAAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(17371..17865)
                     /locus_tag="MJE33_001869"
     CDS             complement(17371..17865)
                     /locus_tag="MJE33_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBY9647805.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNMGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTKIKENEKYKKSINKI
                     KQQK"
     gene            complement(17866..18627)
                     /locus_tag="MJE33_001870"
     CDS             complement(17866..18627)
                     /locus_tag="MJE33_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBY9647806.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFVGVSIAVGMFLVLGLYFKLGGI"
     gene            19329..22163
                     /locus_tag="MJE33_001871"
     CDS             19329..22163
                     /locus_tag="MJE33_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY9647807.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKTGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKISGKPILFLHASIIENLIGRTLNSDEFSINNCNELLKSSKN
                     DSEDIFSTLLGHDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFAETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIVHKANVFIVCATTEDIHSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIKVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSIVPDYIYIGLLKLNENRSNLNKLSELDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNIAKIVLSIIENDLQTSLPKDEVKFISMFLN
                     SMYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     TIELVKECDEGKGVLLLTDMGSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSTL
                     PEMTLDELANSLSSLNPYIGRAAIDSTEINYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNISKYNINLVPHNIESFKMYKNKFSNTKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     FTIIELIDENLKSKFKLGYILHCSFMIERCFKNTTFPYNNITELISKNHELYSLIREA
                     FKPTEAYFNILIPDEEIAYIIDMINEYLNK"
     gene            22206..23450
                     /locus_tag="MJE33_001872"
     CDS             22206..23450
                     /locus_tag="MJE33_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBY9647808.1"
                     /translation="MEDEILKGKIKQLTILALIFIFITPVFAFADTPPVPNSSRAALL
                     IDQETKRILFEKNIDEKMPLASLSKMMTFLLAIEAVDKNQVKETDMVKIDKSTASVGG
                     STCKLKDGDEISLGELMQGLMLVSGNDAAIAIAKHIGKTEKNFVNMMNKKAEEIGMID
                     TYYFNPNGLPIYTDPEHKEPPIENMSTAHDIVTLGKYMYDHYENQVTRITTMQVYNDT
                     KKDFTHYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMVPKDAKNERNHRLIGVVLG
                     DGNKKNRISSSATLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKITVKVDKDLYGV
                     VSDNENINPKVVFKNMNYPIHKGDIVGVAKYYNDSGKFVGSVDVKSESNIGCIPLKDK
                     IKIKVAKINKKLEIKNSVCFKA"
     gene            complement(23865..25607)
                     /locus_tag="MJE33_001873"
     CDS             complement(23865..25607)
                     /locus_tag="MJE33_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9647809.1"
                     /translation="MKKTINNNLFRILNIIVYKNKTKLIVSFIMVFIIAGVNIVIPQL
                     IRCIIDDGIGNKNIFLLINLILIYIFIRILSATFDVILSYLYTSIKNKVTINLKVKLL
                     NHISKLSGRYYSSKKTGNILSIMESDIFMIENFGAEIIFDIIVNVFTAIISLIFLINL
                     QFDLFLVVLFLQLILTFSQNKMTKLISKKISEIRSDSGSLSNLIQEYVSNIMDIVILN
                     SKKNFFNKYLKRERDLIKKDLNLNIIMSSNIAISQILTGLITILIYGIGGYKIINNKM
                     TMGELIAFQQYTGMLIGPCIAIVRANTRIAQSKVSINRVFSVIDEKIDIKVDNNGYRI
                     DNSEIKKIDFKNVSFRYGEDLNLVLNDINLQFEKGEVTGIVGGSGCGKSTITNLIYRL
                     WDINEGDILIDGVNIKDINLKSLRNNIDIVTQDQFVFDDTIKNNIVLNNKISDKELDE
                     ILEVTCLDDFIKNLDKGLDEVVGEKGVKISGGQKQRISIARALVSNKKVLILDEATSA
                     LDNISQKEILNNLKEYIQDKIVIIIAHRLSTIKSADNIYVLDKGRVIESGNDAELLHK
                     DGLYNKLVETADFA"
     gene            complement(25591..26265)
                     /locus_tag="MJE33_001874"
     CDS             complement(25591..26265)
                     /locus_tag="MJE33_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9647810.1"
                     /translation="MKILKVKDLKKIYGKYENEVRALDGIDLDIEPHKFTAIVGASGS
                     GKSTLLHCVAGLDKPTLGNVYLDDVDLYTLNDDKLSEIRRQEFGFIFQSYNLIPVINV
                     YDNIVLPVLLDGKKEDKEYVMRLINKLGIEKQIKKYPNELSGGQQQRVAIARALANRP
                     SVIFADEPTGNLDSKTTNEVMNMLEWCVKEYKQTLVMITHNDNIAKTADRIITIKDGK
                     IDEKNY"
     gene            complement(26302..27291)
                     /locus_tag="MJE33_001875"
     CDS             complement(26302..27291)
                     /locus_tag="MJE33_001875"
                     /inference="COORDINATES: protein motif:HMM:NF012311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBY9647811.1"
                     /translation="MTKVRIAVLDTGLDVNDDDINGNYVYDSNFQLKDLGGKEINDIN
                     GHGTFVSKTILNISKNTQIYPINIFNDSGKTSSLNLLSTLSKLTMSDIQIINISAASI
                     NSMYEKELEKVCNILNSQGRFIICSKHNKYEDKESIPTIFESVIGVVGNENIYKNNQY
                     IYEPENKIQMYANSKDNFIKFKDKVTHFGKNSKACAVATGIVANILKNNPYIKFNELE
                     EILIQKSKSKEDLKIENLNTPSYKSNLIRLDIARKIINVINRNFSKIKIDFEFVSEYS
                     LFNNFTCIGNYNAYNFLTILNKEFNISIDYKEVYLYQLENLDMAVDLIYENLI"
     gene            complement(27339..28583)
                     /locus_tag="MJE33_001876"
     CDS             complement(27339..28583)
                     /locus_tag="MJE33_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBY9647812.1"
                     /translation="MNSSNKYFLDCICEKIINNNQVILSNKVNGRWVKIPIECYEVLK
                     YSVDNALQLGNLYDVFQENEDKKYYERVIDKLEHIGLLSDSILSEFRLERINKISFSP
                     TNRCNLSCDYCCVDSKIDNNDYLDTSQVRKVIDNIVKLNPRILTITGGEPLMRNDFLE
                     LLDYIKEKFKGKIILSTNATLIKEKQIDDIINGLYAIEISLDGYDELSCSEVRGKGVF
                     CKVLNVIRLIKLKKFENVGLSMVVGKNNEKDIEKFNKLNEDLGTTPIIRNFMNIGRGN
                     ENYSKYLNDKLDIDYICKDDYVDESSLCSNMCKAGVNQISIDHKGDVYPCPNLEYKDL
                     KIFNILEFNENILERILNREMDIFNIFDKLKPVNIESCKECKVNLFCTTCPSKIYTLK
                     NNKKMFDANCKKMKNILEPILW"
     gene            complement(28625..31171)
                     /locus_tag="MJE33_001877"
     CDS             complement(28625..31171)
                     /locus_tag="MJE33_001877"
                     /inference="COORDINATES: protein motif:HMM:NF014717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9647813.1"
                     /translation="MINIKLAVSYLKRQKGKTLSLILSIGIAVMLVFSLSVINESQGQ
                     SDINHAYKNSGDYHAFYEDINSDIVKELNGEEDIKEFNDVLKFGEIISKDNGASLNLC
                     SYNKDFLDSTRYKLVKGREPRTSNELVIEQKVLDKMELDVKLNQTIDFQIVNEHINKN
                     NENSIYSKDKQFKIVGILSKSDEYYENLNKYKVRAFTYSNNGKDIVPYELITHMGTLK
                     LNTKDLDSQKVVNLGRKYKLTSNQFYPNTEVSMSMYMKNISKKTTYSIKQSLLPLLCA
                     TLVIYNMFNIIILDMTKQIGLLRAVGASKRNIRQVFFIQSIVVLSVGIVIGLLGGIVF
                     SYLGLMIVYDKSAELTINTNSIIESIIMSIIAVILASVVPIYKAGNLSIIDAIKKTDK
                     LNYIPRLLLLKSHKKVVGIINEIAFKNIFRNKVTSIIIIITISLCGVLFIGRLTSTKF
                     IYGKESDSANITSKSYGNFDIYLGYSPINANYTFSKYDNSLLNEIKAIEDVTDVEPNI
                     YLKGYLRNADLEEDYLDELKRTEINNNNESTVLIRGYNKELLNNIDKYIDKGENVYSY
                     TDRDYKNVLLVNNFYSRIKLSNNTQIIKSPKIGDLIDIKIPVYKDGEYKYINTKVRIA
                     GIMKNSYISEQDGESQSGGIQIIFNEKDLKELTGISDYNKVFVKIKKETDKKVIKEIN
                     NIIEKSGFSDIEGRYVRNHFGDHYNKSSYKLAMICVFGVLLISSINIIFIVRSNIIVR
                     LSEICTFRAIGMSKKNVKRMLIKENMIYGVLSMIVAGIISSFNYYKIVSETNKLNQQV
                     YGINNSIKFEIPIYEILIFGSISVFVCVIAVYFSNKMINKLSIVSGIKEN"
     gene            complement(31379..31540)
                     /locus_tag="MJE33_001878"
     CDS             complement(31379..31540)
                     /locus_tag="MJE33_001878"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647814.1"
                     /translation="MIHIKKIEEKIELQDVEVQADPCKWYYDKHGIISQIDCMVDCAG
                     STPKGTVAY"
     gene            complement(31596..32612)
                     /locus_tag="MJE33_001879"
     CDS             complement(31596..32612)
                     /locus_tag="MJE33_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBY9647815.1"
                     /translation="MKFTIFVTEDCNLRCTYCYEGNDKKKENMSYETADKTIDFICNK
                     ELESNSDYTTIVLHGGEPFINFDLIKYIHRDLVDRLSDKKILFDITTNGTIINDEIIE
                     FIAKNVKNISISIDGKKNIHDINRIDANGVGTYEKVMENGKRLIDKGIYVRARMTYNS
                     NTVKYLYESVYDIAKQGFKNIAPISDFMDKNWDNSHIKILNEQISKIMNIKKDYPEID
                     ISILDKQFINKKKGDCFGGINSYAIDTRGDVYPCTVCVGDKDILLGNIYDGLDDKKIK
                     NLIKIYISKNEECDGCTRLDYCMSTRCKLINKKVTGKFQSAVPIYCGLERVYVETTKK
                     LCSL"
     gene            complement(32609..>33670)
                     /locus_tag="MJE33_001880"
     CDS             complement(32609..>33670)
                     /locus_tag="MJE33_001880"
                     /inference="COORDINATES: protein
                     motif:HMM:NF015983.1,HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBY9647816.1"
                     /translation="NQIFKSNNLNFDMLSNGVAIYFCLTNRCNLFCKHCCVESSSNKK
                     DLLSTYDLKNIVDIISVLNTPHIIITGGEPLIREDFFEIIKYIKQKLPDTKLILSTNG
                     TLIDEHNVDFIITNFEKIDISIDGVDENTCSETRGKGVFNKVISSVNLLKDNGFSNIK
                     LSMVFGQKNSHLNKQFKDLNKKLGTEFVERYFIPTGRGLENKESYLIEDTVLPITIPY
                     ILDNELKMCKDKKNKKMGCCACNAGNSQIFINYDGDVYPCPSLIKDDYKMGNIFDNEF
                     VKVIKENKTRDLKSLIKLESIYPYNFEKCKDCDVNIFCWHCPAILDSVKDNELELDRW
                     CSMMKHNLNTAVWGDEKVN"
BASE COUNT        11595 a         5303 c         3918 g        12854 t
ORIGIN      
        1 atttctatat caattcactt attattacct cacttgttat catcttactt aatttactta
       61 tttctatatc aatttactta ttatcatctc atttgttatc gtcttactta atttacttat
      121 tgctatgtca attcatttat tattatctca cttgttatcg tcttacttaa tttacttatt
      181 tctatatcaa ttcacttatt atcatcttaa ttcatttgct tattatcatt tactttattc
      241 aatttctata tcaaaattag tatttgtact taatacttaa aattaattta aagtctttac
      301 atattattta ttttctattg taatattcat ttccatactt tactaagcta cttccactca
      361 tttattttaa aatttcaatt tactatttct caactctata ttattatttt tcaattttat
      421 attactaatt ttaaagcttt ttagctatta ttcctacaat atttaaacaa ttttttcata
      481 ataaagctat attacaatat attcaaaata tgtaatattt tagatatttt tcgttgacaa
      541 tgttaaattt ttgttttatc cttaattaag gacatgttat tgacatttct gaaacttatt
      601 tgaaatagtt ttttcatttt atttaggaaa acgtattata cttaagaaaa cgtattttct
      661 catttcagat aagtaaaata tttattcttt tggggaggaa atcatggaaa ttaataaaaa
      721 acattttatt caagtatttt taacttgctt aatcttggta gtaatatgtg aatttatagg
      781 gcaaagaact atagctattg gaaaagcctc tatagtactg ttccctatgc tttatgcagt
      841 tataattgga cttataataa caccagattt attaggtaaa aaaattaaag cacttaaaaa
      901 agtggttgga gaaaaagaaa taaatattgc tggtgaagtt gttggaattg cactagtaat
      961 gcttggtata aaatatggta ctacagttgg accaaacatt gacaaaataa ttcaagctgg
     1021 accagcgttt atcgctcaag aatttggtca tatacttgca ccaatcgttg cattacctct
     1081 tgcattaatg tttggaatga aaagagaagc tgttggagct actgcaagta taagtcgtga
     1141 accttcttta ggagttatat cagaaaaata tgggataaac tcaccagaag gtagtggtgt
     1201 acttggtaca tatttaatgg gaacagttct tggtactata tactttagtg tactaggttc
     1261 aatatctatt tatagtggtc ttcacccata tgctttggga atggcctgtg gtgttggtag
     1321 tggaagtatg atgacagcag ctgctggttc attatctacc atggtaccac ctgaaatggc
     1381 agatacaata cttgcctatg ctgcaacaag caatatgatg tctagtataa caggaattat
     1441 attcttagtt tttgtatcct taccatttac taattttatg tataagattt tagaaccaaa
     1501 attttcgaga aataaaaaaa ctaaagaaat taaatctaca gaaggagagt gctaataaat
     1561 gtttgctaat ttaaatttaa agaaaagttc atatcaatta ttggttatgt ctgttattgg
     1621 agttataatt cttataggac aaagaattag cgttggtaca tctatagtta cagctcttcc
     1681 tggtatggtg atgctaatat tagctgctat ggcagctatg attataaaag atttatttcc
     1741 taaatcaatc tttccagctt ttggatttgc aactataatc ggccttttac ttagtatgcc
     1801 atatagtcca acatctgaag tattcttaac taacactaat aatattaact ttatggctat
     1861 aactactcca ctactagctt ttgcaggaat ttctgttgga aacaaaatag aagctttaaa
     1921 agaaatgtca tggaaaatag ttataatttc acttattgta tttacaacta tattctttgc
     1981 atgtgcgagt atagctcata tagttttaag tattcaaggt aaaatatagg aggaatttat
     2041 tatgaataaa gaacaattaa aatctctagt aataaaaact atagatgaaa atagagaaaa
     2101 aatattaaaa ataggtcaag gaatatacaa aaatccagaa tatggatata aagaatttaa
     2161 aactacagaa gctgtttcaa acttttttga gcaagaatta aatttatctg tagaaaaaaa
     2221 tatagcagtt acaggatgca aagcagatgc aaattctagc aagaaaggtc ctcatatatc
     2281 tatacttgga gaattggatg gtatatcatg taaagaacat aaagatgcta atgaaatagg
     2341 tgcttctcat acttgtggac ataatattca aatagctgga atgttaggtg ctgctgttgg
     2401 tttagtaaaa agtggcgttt tagaacattt agatggtaaa gtatctttta tggctactcc
     2461 tgctgaagag tttattgagt tggaatacag agagcaatta aaaaaagaag gaaaaataac
     2521 ttattttggt ggaaaacaag aattaataaa aagaggttat tttgatgata tagacatatc
     2581 tatgatgttt catgcttctg atttaggaga agataaggct ctagttggtc ctgagagtaa
     2641 tggatttgtt ggaaaaaagg ttaaatttat aggtaaagaa tctcatgctg gttctgctcc
     2701 acatgatgga ataaatgctt taaatgcagc aatgcttgct ataaacaacg tgaatgcact
     2761 tagagaaact tttaaagagt ctgaaagagt tagattccac cctatcatca caaaaggtgg
     2821 agatatagta aatgttgttc ctgctgatgt tcatatggaa tcatatgtta gagctagaac
     2881 tataaatggt atgattgatg ctaatgaaag aataaataag gctttgatgg ctggtggaat
     2941 ggcagttgga gctgatgttg agataactga aattcctgga tatcttccaa tattacgata
     3001 caaaagtctt gataatttat ttaaaaacaa tcttgaagat ttaggtctaa aaggaaaagt
     3061 tattgatggt ggagatttta caggctcttt tgattttgga gatgtgtctc atataatgcc
     3121 tactcttcac cctatgattg gtggtgtaaa aggagctctt catacaagag attttgaaat
     3181 agttgatgaa gatttagctt acataattcc tgctaaagca atggcactta cagttgtaga
     3241 ccttcttttt gataatgcta aggatgctaa agatatatta gataccttta ctccagttat
     3301 gactaaaaaa gaatatctaa cattcttaga aaaacacgat aaaactttat aagtctattt
     3361 ttataatagg gtggtatcta ctgtaaaagt tataccactc tttgtttttg taacagtata
     3421 cttcttttct tttagttttt agattaaact aaaatatatt atcctcctta aaacttttag
     3481 agctacgttg aaatatcagt agaactatgc tatattcttt ctaattaata taaatttcta
     3541 ttctaattca aggtggtgaa aaaatgttta atgcaggaaa tcgaattata aatacatggt
     3601 tgtaccctat tcaagatggc tttgtcttga ttgatactgg atatgaaaat ggttttaaac
     3661 atttcaaaaa acgaatttct aaattcaaca taaaaatcaa agatattcga tacatatttc
     3721 taactcatgc tcatgatgac catgttggtt ttctaaatca gattctagac gaatctccta
     3781 atacaaagat tgttatgcat gataaagctt tagaaattct atataaaggt caaaactctt
     3841 ttattggtgg ttgtaccagc cgtactgcct ttctcttttg ccaaattatg aaactatttg
     3901 gaaaaggtga gcatagtttt tctccactta aacaagaatt tgaaaaaagg tgtattcttg
     3961 tgtctgacaa gaatcgtaaa gaaattgaaa aagaactaaa tggaaaaatt atagatacac
     4021 caggacatac tgatgattca atttcactac taattgatga tggtgtttta ttttgtggag
     4081 attctgcaat gaatggattt ccaagtactc acaaaattac catatttgca gaaagcaaac
     4141 tagaatttat tcaatcttgg aaaacaatta tcaatacaaa acctaaaatg atttatcctg
     4201 gacatggaaa accatttgaa tacagtaaat tagaacaaaa tctaatctat gtaaataaaa
     4261 taaatttata tcctctacat aattcaaaag atattcacag atttttgtag aaaatacaaa
     4321 gtaaatttct ttgattaatt aaaaaatcta acactttact acgctactta ttttaataac
     4381 ttaatcttcc actagctttt cataaaattt tgttttaaaa ttaattttaa agcgcatatg
     4441 ttatttaaat acattagata taattcgtca ttaatctact taaagcatat ttaactaaca
     4501 ccactcaacc agaaaattca gaaaactgca ttgacacagg aaaaagttta ttttatactt
     4561 tttttaggac atattgtgga cacgtcttaa atcgacaagc agaatttaaa ttgtaagatt
     4621 aaccacaata cttatttttt aattatattt ttaaacatta gggaggatat tatgaatgat
     4681 aacgtagtta agaagaaaaa cttttttgac aggacattaa acagaataga aactgttggt
     4741 aataaactac cagacccagt tacaatattt ttagggttat gtgtactttt acttatacta
     4801 tctagtctag ttggttctat ggggatatca gtagttcatc ctggtactgg agaaactata
     4861 accgctgtaa atttattgac tgtagaacag cttcaaattc ttttaggaaa catagtcagc
     4921 aatttccaag ggtttgctcc tttgggtcta gttctagtaa ctatgatagg tgctggtgta
     4981 tgtgacaaaa caggacttat gacagcaaca atcaaagcat cagtaagcaa gattcctgaa
     5041 acaagagtaa ctcttgttgt aatgactata ggtatgcttg ccaatatagc ttcagatgca
     5101 ggtacaatat tatttccacc attagctgca cttgtatatc ttggtgttgg tagacatcca
     5161 cttataggtt tattttctgg atatgcagca gtctgtctag gatttgccgc aaacattatg
     5221 ataagtgtca atgatatact tgctgcaagt tttacagttc cagcagctca aatgattgat
     5281 tctggatttc aagccaatgc aacaatgaac ttatttttta tgatagcatc aacttttgtt
     5341 ctaatacttt tggcaacatg ggtaacagaa aaaattgtag ctcctagatt tggtaaatat
     5401 gaaggaaatg cagaactaga tgttgatgga agtcttagtg atgtagaaaa gaaagggctt
     5461 aaaaaagctg gtataagtat attaatatat gtggctatta tagtagctct tagtgtaatc
     5521 ggtaaaaaac cattcttagc tgacccagaa acaggagatt tactatcaaa caatgctcct
     5581 cttatgaaag gtatggtacc tataatagca ctagcatttt tcatacctgg tgttgtatac
     5641 ggaaaaacta taggtaaaat aaagaaggat aaggatgtag tctctataat ggctagtgca
     5701 atgagtgata tgggtggata tataatacta gcatttgctg cttcacaatt tttgcaatta
     5761 ttcacaaata gtaatttagg tctgatacta gctgtaaagg gcggagaatt tttaaaatca
     5821 gcaggaataa atggaccttt attgttgata ggatttatta tattatcctg ttttataaat
     5881 ttatttatag gtagtgcatc tgcaaagtgg gctattcttg cacctatatt tgtaccaatg
     5941 tttatgatgg ttggatacaa tccagcactt actcagatgg cttatcgtat aggtgattgt
     6001 ataacaaatc cacttagccc attatttccg tacttcccta ttatactagc attcacaaga
     6061 aaatacgata aagatgctgg tatgggtact gtaattgcta atatgatacc atactctata
     6121 tcattcttaa tagtgtggac tatactacta atattgttta tggtatttaa tattccacta
     6181 ggacctggaa tacttccttc atatagcatg taatttattt aaatctttct aaaaggagaa
     6241 ataatttatg ttagataaaa atagacttat aaataatttt atggatatgg ttagaattga
     6301 tagcccatct aatcaagaat tagaaatgtc taagtggttg gtaaactact taaaagaaag
     6361 aaatatagat gccattatag atgatgcagg tgaaaaatat ggtggtaata caggtaatgt
     6421 cattgcctat ataaaaggtg aagaaggaag tagaccactt tgtttatgtg cacacatgga
     6481 ccaagttcaa ccttgtttag gtgtaaaacc aatactagat ggtaatgtag ttagaagtga
     6541 tggaactact acacttggtg cagatgataa agctggaata gctgctatac ttgaagcttt
     6601 agaacatgtt attacagaaa aaataccaca tagagatatt tatctttgtt ttacaatctg
     6661 tgaggaagct ggtatgcatg gtgtcaaaaa ctttaatcct gacaacttgc cttgtaaaga
     6721 tatggtgatt ttagattctg gtggagctat tggttcgata gcatacaaag ctcctgctca
     6781 acaatctata aaaatctctt ttcaaggaaa gaaagcacat gctggtatag aacctgaaaa
     6841 agggttaaat gctatattag tagcctctca tgcaataagc aacatgcaca taggcagaat
     6901 agattcattg actacttcaa atataggtga aatagaaggt ggtggagcta caaacatagt
     6961 aactgataag gttacattga ctgctgaaat taggtctcat atacctgaaa ctttagagta
     7021 tgagcttaat catatggaaa aatgttgtaa ggacgctgca tctaaattta atactactta
     7081 tacttttgaa cataatatgt cttaccctag ttttgaactt agtagagata gtcatgtttt
     7141 caagttaagt gaagaagcaa taagacaagt tggtgttgtt ccaaatccaa tggtaattgg
     7201 tggtggaagt gacgcaaata tattagctaa tcttggatat aattgtgcta tattaagtct
     7261 aggtatgtat gatgtacaca cagttaatga atatgtaaat atagacgaat tatatgatac
     7321 tgccaaaata gtttatcata tgattaaatt ataaatatca taatagaact ctttaaatta
     7381 taaaaataaa aacctatgta tcctcaactt tataagttat atattagagt tgttttacat
     7441 aggttttagt atttttatat aataatttgt tttaacattt ttcataataa gctttgatat
     7501 tttatatagt aatttgttct aacatttttc ataataagct tcaatatttt gcataataat
     7561 ttattctagc atttttaata ataggctcta gtattttata ttaataattt attctagtat
     7621 tttacataat tatttgttct aatttttttc ataataagct ttaatacttt atatagtaat
     7681 ttgttgtaga attttataca atataatgat ttatacagtt ttataaaata tcttaaaaca
     7741 gtatttcagt aatatttttt ggtcttcctc caccaatact agtctctttt gcacatattc
     7801 gacctaaacc tgcatttact attttattta atatacgtct tgcacttctt tcacttacat
     7861 ttagatactc tgctaactct tttgaatcat aaatcttact ttttctattc tcatttatag
     7921 caataatttt tgcaactgac tcacaactca ttccagtctt tttagatatt tcgattacct
     7981 tttcatcaga tagcatcaaa gaataattta attcattttc ggaagctaaa ggtcctctta
     8041 tgttttcatc ttcatctacc aaatatatac caatttcttt tgaatctatg gagtgtctta
     8101 atgctttatg tgcattattt tcagcctgat acgcagttac tccagtacca attcctatac
     8161 taagagaaaa gcccatgtct tttatctctc tctttagatt gaatagctta tcataattat
     8221 actcattatc agctacccct ttatgaacaa atacaacata atcatttctt ccaagtaaaa
     8281 ataaggaacc ttgaatgctt cttacatagt ttactacaag cttatccata tctgattttt
     8341 ttatcatgtc agaataatag ttatctatct tctctttata atctattaaa ctcaatattt
     8401 caacagcaat ttgagaaaat cttgccttac ttagagcata ttcactcttt accttgtcat
     8461 aactttcttt gattaattgt atagtagctt gtaaacgaaa tacaggatag cctctttctt
     8521 ttaattcatt atatactgca ccaaaaccag ttaacattat atccgtctta ttttcttcaa
     8581 ataattttat atgccattct acaagttcag actctcctac ttccaaagaa aatggattac
     8641 aaaatacact cgttgcttta atatcaaatt ctttaagtgc atcttccaca acatatctat
     8701 caactacatc tatactgaat ttatctaatt ttatatttgc actttttatt gcccaaaatg
     8761 ccttcattat actcgtccca cttctcggta caaatgaatg tggcttactt atctcatact
     8821 tagattttat aaattcataa actgcacatc ctgtaaatat aatagcatca cattcgtctt
     8881 cacatttaga aataacttct attgtatcta ctactttttc tcttacatat attttagtct
     8941 ttaaatcact gtctatctgt tttaaatcat cctttatctt aaacccagag tctgaaggac
     9001 ctataatacc cactatcata aacttcgctc ctaatccata aatattatac tttttaaaaa
     9061 ttatatcctc actattttct acttctattt aattattttg aactaatcca tcctacctac
     9121 atttagaatg gaataattag cttatttttg ataaagtaac ttctcaaaat cagcacttgg
     9181 aataggctta caataataat aaccttgaat taccacacaa tctaaactca ctaaaaattc
     9241 agcttgttct tttgtctcta caccttctgc cactacatcc atatctaaat cttttgccat
     9301 ggctatagtg tgctttaaaa ctattttttc tctgtctgtg attccttcct cattaaagaa
     9361 accacaatct atctttaatg tatcaatatt aatatttttt aacatattta atgaagaata
     9421 tgctgaacca aagtcatcca tatttaaaac aataccatcc attctaaggt cggctattat
     9481 cttagtaaat aattttatat ttttaataaa taaactttct gtaagctcta attctaaaaa
     9541 agattttggt atactatatt tatttattaa acttaaaaca atttctttaa aatgtggatt
     9601 atgcaaatgt aatcttgata catttacaga gataggaact ggctcttcac ctaaatctat
     9661 ccattttcta atcaaagaac atacttgttc ccatacatac atatctaatc tgataataaa
     9721 cccattcttc tcaaatagtg aaataaattt aattggaggt attatacctt tttctggatg
     9781 aacccatcta accaatgctt ctgcaccaac aattttattt gcattaagat tatacttagg
     9841 ttgtaaatat acatggaatt gcccattttc aagtgcgtat tccatttccc tttccatctg
     9901 tttttcttca agtgcatcat ttcttatagt atcatcataa aatatatagt tgttgatact
     9961 ttgtttcttt acttttttgt gagcatatcc tgccatttca cacgcatttg taattggaat
    10021 atgattatcc aaaattctat aaactccaaa acatggtact aacatatatt caaatttttt
    10081 tctatttaag ctatttttaa tcttataaat taccttatga atatctctat cagagtaact
    10141 ctcataaaaa atgcaaaata catctgcata catacgacaa catataccat tagaaccaat
    10201 gtttttttga atcaactctg caacatatat taaagcttta tctcctcctc taactccata
    10261 aatgtcatta attaccttga acttatctat atccatcatt ataattgcat aacgcttgtc
    10321 aggatatctt ttaagcatta aacttatttg ttcacaaaaa ctttcataat tgtatagtcc
    10381 tgttaatgta tctttttggt ttttatgtag ttgcataaga taatctatat tttttatagc
    10441 aactaaaata tgtgtttgtt tctcacctaa cttattttta ctactaatct ttaacatata
    10501 aattaatgcc caatagtact ttttatctat tcctaaacgt ctatactcaa aatttagttc
    10561 taaatcgtct tgcttgctat tattaagact agcaagagat tttagaaggt ggttatcatt
    10621 aaataatatt atatcatcag gatgaattaa atgttttttt atgtatgaaa aagttttttc
    10681 aagattttcc tttataggaa aactaaaaat attgctctct gattgataaa ttggagtaat
    10741 taaattcgta tcaatgcaaa tagataaaat tatatcatat gtattataag ctaatttttc
    10801 ataagcctta gaataccctt ttatcattaa atcatcaaaa taaaccttat caactaactc
    10861 tttatgttta aataaaatat tattcgtcga atcagctaag tcactaatta ctttcatttc
    10921 attatttcta agtttgttct tattataata cctcttctta tcttggtaca ttctctcatc
    10981 agcaacttta attagcttat gtatatcaat attatcgtca ctccaaatac tgcccactgc
    11041 tacactatat atcatctcat ccttaaattc ttcacttaac caactcacta attcttcaaa
    11101 tagttgtttt gatatatctt cacataatac aacaaactca tctcctccaa tacggtaaac
    11161 attataagaa tgaaagtatt tttttattat tacacatgct gacacaatag cagagtcgcc
    11221 attagcatgt ccaaagttat cattaatatc ttttaaccca tttatatcta caaatacaac
    11281 tcctatggat ttaacttttc ctttttgaaa attatctata tacttaatat atttatttct
    11341 attatatgca ccagtcaaga agtcatagta gctcatataa tttaatttat tttctacttt
    11401 tctttttttc atctcagtta ataaaaaata agatagggat gtcaaaagag aggtatctcc
    11461 aacagcttta tgtggattat caactccaat aaaccctact actttatcat cttgttttag
    11521 tgaagatgtc attaaacttt caattttttg aggttttaaa atttcatatt ctattggctg
    11581 ttcttctttt atattttcta tatcatcaat tattacaaaa cctttttgtt cgaataataa
    11641 aaaccaattc tttaattcat taatatcaag atttgttagt gcctgttttt gttcatcaac
    11701 accttcattg caccattcat atgtattagt tacttttttt ctatcttctg atacttcaaa
    11761 aacatatgct ctatcagctt tatgaaattt accaatattg gttaatacaa gatttacagc
    11821 atcagaaaaa ttagattctg ttataagatg tttaatacat ccaactaata actcctttat
    11881 ctctaatttt tctgctattg acttacttat atcttctttt tctgtaatat caagagctat
    11941 ttctaatcgt gcatgtttgc cctcccattt aattaacttg tcctttaata taaaatgtct
    12001 atttgttcga gtattcgtat tttcccatat gtaatattca tcttctttta aaatagagtt
    12061 tgtacaaaat tcgcagggag aatctttccc ctgtaatgct ttataacatt ttaaatggga
    12121 tatatcatta agtcccattg cctttttacc aatttcatta atatataaaa gttcatatgt
    12181 atctaaagaa gaaacatata caatttcatc caactcatct attaaattta ttacatccat
    12241 ttatatatat ctccttttat tcattttcca gattgaattt ttaaagcttt taaaattttg
    12301 ctatatattt ggcaatgata ttatacttct ttatgtttta aaatagtacc ttaatgaggt
    12361 tctatttttt agtaaataga attattattg tgtcaatcgt acaatagagt tttgtaatac
    12421 ctaatttaat atatttataa ttaattatct cattatgtgt ttataattaa ttctcatata
    12481 aatgacaaat ctccacctca attaagtatt ccaatcatat atgaagataa tacttcattc
    12541 atttaaaaat ataaaactat tatatattaa aatttcttca ataataaatt atctagattg
    12601 ttactttgat atattatata atatcacaaa ggctgatata aaaagacaaa aagggcaatt
    12661 tttttatatc aaaaaaaaat gttaaattga tattatccat atataaattg atattatcca
    12721 tatagatgct atatttaaca attctactta cttcccttac acttattagc atatagttat
    12781 taagagtttg ttgcaaaaca acacttagca cactttaaaa agtgtggtct aataagtaat
    12841 caacgaagaa gacataataa agcataaaat atgatatact tttccacgcc atcaaaactg
    12901 aggattttat tattaatata tgctaaaata tgcttaataa attaaatttt aatgggagaa
    12961 tagttatgaa aatatttagt ttcttgttgt ttgtgatagg aatatgttta tgtattgctg
    13021 gatttatagg acattaccca gtatttgttg gtacaacatt gagttttctt gcaattgcaa
    13081 tgatagctgt aagacaagtc aaaactaaga aaaaaatgaa ataggcaaat cctaacttta
    13141 caagttaata ttacataagt aattagtcta aaattggata ctttttctat tacagaaata
    13201 catcatccac tttgtatttt cttaaaaaaa taaaatagct atgtaaattc aagattagat
    13261 agattaactc tctatccatt tcattgtaaa atatacatag ctatttgtat tcgatttaaa
    13321 atacttagat tttcttacat aacaaataat ttctatctga ttattactca tattcattta
    13381 gtaatcactc agcaactaat catttagtat ctattcatcc gccatacggt aacctacccc
    13441 aacctcagtc aaaatatatt ctggctgagc aggattcttt tcaattttcc ttcttatact
    13501 tgccataaaa actctaagcg actgattttc actttcatag taatttcccc aaactttatc
    13561 tattataaac ttatgggtta agactcttcc tgaatactta gacatcaact caataatctt
    13621 atattcgatt ggagttaaat gtatttcttc accattaatt gacacaatat gattttcata
    13681 atctatcttt aggtttttaa cttcaaaaga ctttggagca tcattattta tattatcctg
    13741 ttgagcttta tttctaagag ccacacgtac tctagccaaa agctctgtta cactaaaagg
    13801 tttagttaaa taatcgtctg ctcctccatc taaagcttcc accttatccc tctcatgctc
    13861 tctcgctgaa acaacaatta ttttattagt ctttgcacat gccctaactc gtcctattac
    13921 ctcaatccca tcaatatctg gtagtcctaa atccaaaatt atcaaatctg ggtcaagaga
    13981 atgtataagt gatatagctg ttcctccatt atctgtctct acacacctat aattttgagt
    14041 ttcaagagat acttttataa atttacaaat tggcttatca tcttctacaa ttagaactaa
    14101 tggctttatt tccacttgtc tttcccctcc tcatcataca taggtatgct aaatctaaat
    14161 gtagctcctc cagaaggatt attaaaggcg acaatttctc caccatgagc ttctactata
    14221 gatttacata tctccaatcc aagaccaata cctcttcttt caagatttct agacttattc
    14281 ttagtataaa acctattaaa aatattcccg atatcttcat ttggtattcc ttttccattg
    14341 tctgcaactt caaatagtac ttgatttgac tcttttttta cagttagttc tatcttagaa
    14401 tttttaggtg tatgccttat agcattatca attagattta ctaacacttg ttctattaat
    14461 aatacatcca catgaacgat tattatctca tccggaatat ttgtttttat gtctctacta
    14521 ttggcaaact ttttgactct taatatagat tcagctatta tttcatcaac aacctctggt
    14581 cgtttttcaa tatccaattt tccttcatct attctggtca tactaagtat attctcaaca
    14641 gaatgagtta gccaactcgt atcttcatat atatttttca ataattctac tctagtttct
    14701 ttatctatca catcatcatt ttcaagtatt gttgaagatg aacccaatat acttgtaagt
    14761 ggagttctta aatcatgaga aattgaccta agcaagtttc ctcttaacct ctctctctct
    14821 gcctcaagat ttgctttttt atttttctca aataaatttt ctctatcaat tgccaacgca
    14881 acctgtgttg aaactgcttc taatagtatc ttttcatttt ctgtaataat atctatctta
    14941 aaacacgcaa cacctataac accaagaaca tttgattctt gaccttttat agggaaataa
    15001 tatgcactgc tgtttttaca atattttgtc ccaattccaa ctgggttacc tgtcctaaaa
    15061 gcttctttta tagcttcctt ttctaatact gaatggaata tattctcact tccatcattg
    15121 ttacaaatat aaatacttgg cttacctaga ttattattct catctgcaat agtcactatt
    15181 acagttctat ttaatatatt tactaaatta gacccacaaa attcaataat ttgattttta
    15241 ttttttgctt taagcaacgt tttgttattc tcatagagca atcttatcat tttttctctc
    15301 ttatgagaaa gtctcacttc tctttttatc ctagaagtta aagtacttgt tataattgct
    15361 gccattagca taatacaaaa ggtcatagga taatcttttc tataagctaa aaaagtatat
    15421 aatggatgcg taaagaaaaa attaaatgat agcactccta ttactgaaga aagtattcca
    15481 aaaaaatatc cataggttat acttgtagca aatagtacac ctaatatgta tactagtata
    15541 atattagatt cattaaaacc taccttttca aaaactactg atattccagt agcaattgtc
    15601 ataaccaata tagtttttat gaagtcctta ataattatct ttttactgag atagccatct
    15661 tcatcataat catactcttt taagtcttca tgttgtagtc gttccttttt catttactac
    15721 aattcctcct tattgttact gtttaaattg gctctatttt atataataca attttatcat
    15781 aataaattca atcaatacat ttaattaatg tacttaaatt actaaaaggt gctgtcttaa
    15841 aatgaaaata aatctctatt tcaaaacagc acctatatat ttaaattact aatttagttt
    15901 ttacatactt aaatcaatag ttttattcaa tttcataaat tataatactt caattataaa
    15961 acatttctat ccaacaagca taaatataaa gtgggcacat atacctgcac ataaaccacc
    16021 tatagtatgc gaaagtatcg ctttaccagc caatggtctt gcatctaaag catccatcat
    16081 acctatgtgt gtacttaaat atccactcca acacataccc attgctgtga atactgctat
    16141 atcattagga gttatagcgc ctgattttat aaactctggt actaaagata ttgctgctcc
    16201 aacagctcct aatgcagtta cagggaatgc tattgcctct ggtgatgtga atccaaataa
    16261 tggttctatt atgaagctaa tcttatcacc aatagcaggt aataatttta taccttcata
    16321 tgcagctcct gtgtaaacag cttgacctgt agcagggtct gtagatggtc caaaagttaa
    16381 taacataact aatgtacaaa ctactaaaac tcctggtatt atagccatac ccatttcaac
    16441 acctaactta ccaccttcaa gtatagcatc caatgttctt tggaatacat taccatctct
    16501 tatttctcta aactcttcaa gttttgcacc tttatcattt atttgctttt cagtatcatt
    16561 tttagggtca taattataat actttttagt aaaagtcaac ataattctaa cactaataat
    16621 actacctatt attgccgcaa cattacctat tatagctggt agtacatatt ctttaccttg
    16681 agctatcata aaagttgtta caataagtcc cataccaaag gctgttccta aattacataa
    16741 agcaggtact tgatacttct taaaatattg tgtaaatgtt ttgtctttag caaatggtat
    16801 aatagctgga ttatcagata aatatgttgc aactactcct gctatactag caccaggtaa
    16861 gccccatata ggtctcataa gacccttaaa tatcttatta actaaagata ttactccaaa
    16921 ctctgaaagc aatgcactaa gtgcacctgc aagaactgcc atagccatta ttaaaaatac
    16981 tgtgtctaat aaaagcgcat gtgctgtact cattattact ttaaacatca ttcctgcacc
    17041 catgatgctt cctacatatc caaatcctac cgccaatagt acaataaata cgaaggtttc
    17101 catacttact gccttgactc ttctcttatc cgacagcttt tccatcttga aatcttcccc
    17161 cttttaattt taaattaagg ttcctaacat tggtacaaat tttttggtac aaactgagtt
    17221 ctaaaactta cactcgtaat gcaagttgtc agttttttga aataaaaaaa aattacgttt
    17281 atagaataag aatatcctct ttaatttgga tactcttatt ctattttatg ttattatatt
    17341 atcatccttt tgtttaaaag tcaatttttt ctatttttgt tgtttaattt tatttatact
    17401 ctttttatat ttttcatttt cttttatctt tgtaagtact ccatataagt tgacttttct
    17461 aaatacagta gttataaata ccaatcctaa tgcaattgaa cttgcacacg ataatgtact
    17521 caccccatat tctacagctt ttatataatt attggtcaaa acagaataca tagtattata
    17581 tattccataa cctggtacta atattattaa acagcaaaca cttaatgtag ttgctggagt
    17641 catataaatt cttgcacata actcacaata tatcgcaaaa caaactgtag ctataaaaaa
    17701 acataagttt tctgttactc ccatatttaa aagaaattta tacgaaaacc aagctatact
    17761 tccacctatt cctgctagta ttaagttttt tcctttcata ttaaatagca ctccaaagct
    17821 taaggcagta aaaaaagctg ctattacttc tataaacaaa tgcacttata tcccccctaa
    17881 cttgaagtac aatccaagta ccaagaacat tcctactgct atagatacac ctacaaaaaa
    17941 tacttctgct aattttgcaa gaccagaaat caactgtcca tctaataaat ctcttaatgc
    18001 attagtcaaa gcaagcccgg gaactaagag cattattgct cctgctatta aatggtctaa
    18061 cgtctttatg aatccgagct ttaaaattaa aattgaacaa gaagctataa taactgctcc
    18121 aattgcattt acaaaaaagt cattaaggga agatttactt aaatacataa taacaaactt
    18181 agtcagaatc ccaacagtaa atgcagctat aaattcttcc acatttcctc caaataatat
    18241 cgaaaaagtt gctgctgcaa tacctgcaaa aaatattgtt attaaatccg agtaatagtt
    18301 atcttcactt atttcatcta aaattttctc acataattca atatctaaat tatttatact
    18361 tattgctctt gataatgagt taacttcatg aaccaagttt aggtctactc ttcttgagct
    18421 tattctttta attgctgaat gtaatttccc atctacaaat acagatgcca ttacagctgt
    18481 tggagttgca aatacatcta cgtgatctat tccaaaagat tgacatattc tagctatcgt
    18541 ttcttcaact ctataggttt ctcctccact ttgcaacata gctttacctg catttgagga
    18601 aaagtttaaa attctatcta tatccacaaa acatatctcc cattctttta tagtattatt
    18661 catattttaa tacaagatat tatttattta aactatttta aataattcat atccaatatt
    18721 tacattttat gaaaaaactt gttttttatg cttgtcaaaa tcaaatttta agcttgttta
    18781 caccataaaa tggctgaatt aatatttgac aaacactttc aaccatttat ataaccttct
    18841 aaattgtata actttttact acaaataaaa ttatatctct tttaattctt tctcttgagt
    18901 atttagctta tagtaacttc atatttatat atgtacaata taaaacatta tttttatgat
    18961 acaaaatata atttgtaaat gtatatatgt attaactgtg tatatataaa ttattcttaa
    19021 ccttatcagt tttttcatat atcttcatct ataccgacca tatattaata aatgatatta
    19081 catagactta agtacattaa aaaacttaaa tttagttatt taacaacata atactctaat
    19141 ttaatctata aaaatccata cctaatataa tgcaactctt attttaacac catctattac
    19201 acaaaacaac atattaaaaa gtaaaataca aaatatccac tataaacctt attttaatac
    19261 tgaatatata ttataatata tttaatgtag cttaaatata gtttaaacat aaagaaagga
    19321 aacggtatat gctaagaaaa gataaagttc tattatctct acataaatta tgtgaaaata
    19381 tgactttaaa agaccttcaa tcagataaaa caggattcac tacaatagaa attgctaacc
    19441 attcatctct tgatagaagt aataccagta aagaattaaa taacctatac aatgaaaaaa
    19501 aagttctgaa aatatctggt aagccaattt tatttctaca tgctagtatt atagaaaatt
    19561 taattggccg tactttgaac agtgatgaat tttctataaa taattgtaat gagctactta
    19621 aatcaagtaa aaatgactca gaagatattt tttcaacatt acttggacat gattctagtc
    19681 ttaaactacc tatagaaaaa gctaaatctg ctatactcta tcctccaaaa ggtcttaata
    19741 cgttactaat aggacctact ggtgttggaa aaagtacatt tgctgaaaca atgtacaaat
    19801 atgctgtaga aagtaaaatc ttttcttctg aaagcaaatt cgtagtattt aactgtgctg
    19861 aatatgcaga aaatgctaac ttattattaa gtaatttatt tggttatgtc aaaggttctt
    19921 ttactggtgc taataaggat aaattcggca tagttgcaca ggctgatgga ggcatattat
    19981 tcttagatga aatacacaga ttaccaccag aaggtcaaga gatgcttttt cttttgatgg
    20041 acaaaggcat atacagacga ttaggagaat ctgacattgt gcataaagct aatgtcttta
    20101 ttgtgtgtgc cacaacagaa gatatacatt cttctctgct tgggactttt ttaagaagaa
    20161 tccctgttac aatcaaccta cctgctttac aggataggtc tttaaaagaa agattatctc
    20221 ttattaaata cttctttatt atagaatcaa aatacaccaa tgcaactata aaagttcaca
    20281 aagatgtaat taaatcatta ctcctttatg attgttttgg taatataggt cagcttaaat
    20341 ctgacataca acttatgagt gcaagagcat tcttaaatta caaaactgga cttaaggata
    20401 atatagaaat agatttatcc attgttcctg attacatcta tattggtctt ttaaaattga
    20461 atgaaaatcg tagtaatcta aataaattat cagaacttga ttatatgctt ttttatgaat
    20521 ttaatggtaa taacttagaa gttgaatata caaaagaact atctaaatac aatatagata
    20581 catcaaacaa tggatttaaa tactctacag agattcctta tctggaaaat gatataagga
    20641 gtatgtttat aaattacatg gaaaagtact ctacaaaatt actttcgata tcttcagtaa
    20701 ctcattctga ttatacaagt aatgagattt ttaaagtaat caatccaaag atttactatg
    20761 ctgttgaaca ttctttaaaa gaggctgaaa aaatatcgag aaacaaatac acaaaacaaa
    20821 cttatattgc tctttcaatg catataagtg cacttataga aaatataaat caagacacat
    20881 ataatcctaa gtacaattta tataatgata aaattttctt agataaagat ttaaatatag
    20941 ctaaaatagt tttaagtata attgaaaatg acttacaaac atctttacct aaagatgaag
    21001 taaaatttat atccatgttt ctaaatagca tgtatcttga accaaacaat caatctgaaa
    21061 gtataggagt cataatactt gctcatggaa attcaacagc aagcagtatg tgtgatgtag
    21121 caaattcatt gcttggtaca aaccattgta atgcaattga catgcctctt gatgtaaaag
    21181 taagtaccat acttgataaa actatagagt tggtaaaaga atgtgacgaa ggtaagggtg
    21241 tacttctttt aacagatatg ggctctctta tagcttttgg agaacttatt tcagaaaaaa
    21301 caaaaatcaa aactcgctca attgaaatgg tatctactcc aatagtttta gaagcagtaa
    21361 gaaaaagtac actaccagaa atgactttgg atgaattagc taattcctta agctctttaa
    21421 atccatatat tggacgagca gcaatcgatt caacagaaat aaactatcct gaaaagtcaa
    21481 gagagtatgt aataataact acttgtataa caggatatgg ttcagctatg aaagtagcag
    21541 actttataaa ttcttcactt aaaaatatta gtaaatacaa tattaattta gttcctcata
    21601 atatagaaag ttttaaaatg tataagaata aatttagtaa tactaaaatt ctggcagttg
    21661 ttggaagtgt agattttaat gtagccaaca ccccttttat acacatagag gaactactaa
    21721 atggatatgg cctagatatg ctaaacaaaa taatagaagg tacattcaat gccaatagta
    21781 ttacaaatat aaaagaaaca aatctatcat acaacagttt aataataaac tttatgaccc
    21841 aaaacttaga gcttttggac tctgttaaat tattcaacta tgtcaattct tccttattta
    21901 caataataga attaatagat gaaaacttaa agagcaaatt taagctagga tatatactac
    21961 attgctcttt tatgatagaa agatgtttta aaaatacaac ttttccatat aataacatta
    22021 ctgaattaat tagtaaaaat catgaactat atagtttaat acgtgaagcc tttaagccaa
    22081 cagaagctta ttttaatatt ttgataccag acgaagaaat tgcttatata atagacatga
    22141 ttaatgagta tctaaataaa taaaatttat agcacttatt ttgtgcatat taattaatat
    22201 atgaaatgga ggacgagatt ttgaaaggaa aaataaaaca gttaacaata cttgcattaa
    22261 tttttatatt tataactcct gtatttgcat ttgcagatac tccaccagta ccaaattctt
    22321 caagagcagc actacttata gaccaggaaa ctaaaagaat attatttgaa aaaaacattg
    22381 atgaaaaaat gccattagcg agtttaagta aaatgatgac ctttttattg gcaatagaag
    22441 cagttgataa aaatcaagtt aaagagactg atatggtgaa aatagacaag tctacagctt
    22501 cagtaggagg ttctacttgt aaacttaaag atggtgatga aatatctcta ggagagctta
    22561 tgcagggtct aatgctagta tctggaaatg atgctgctat agccatagct aaacatattg
    22621 gtaaaactga aaagaatttt gtaaatatga tgaacaagaa agcagaagaa ataggaatga
    22681 ttgatacgta ttattttaat ccaaatgggc ttcctatata tactgaccca gaacataaag
    22741 aacctcctat tgaaaatatg tcaactgctc acgatatagt tacacttgga aagtatatgt
    22801 acgaccatta tgaaaatcaa gttacaagaa taaccactat gcaagtatat aatgatacta
    22861 aaaaggattt tacacattat aacacaaatc ctctgcttgt ttcagtacct ggtgttgatg
    22921 gaataaagac aggatataca gataatgctg gatattgcct tgcattttct atgatggtac
    22981 ctaaagatgc taaaaacgaa agaaatcata gattgatagg tgttgtactt ggtgatggta
    23041 acaagaaaaa tagaatttct tcttccgcta ctttattgaa atatggtaaa gataatttcc
    23101 attctaaaaa gatagcacat aagggtgata ttatagagac tccttgtgtt gatggtattg
    23161 atgattttaa gataacagtt aaggttgata aagatttgta tggtgttgtt tcagataacg
    23221 agaatatcaa tcctaaggta gtgtttaaaa atatgaatta tcctatccat aagggagata
    23281 ttgttggtgt tgcaaagtat tataatgact ctggtaaatt tgttggaagt gttgatgtaa
    23341 aaagtgaaag taatatagga tgtattcctt tgaaggataa gattaagatt aaggttgcta
    23401 agataaataa aaaacttgag attaaaaatt ctgtttgttt taaagcatag taaagcatat
    23461 tattaaaagg gtatgttaaa tttataaaga ctccactttg tggagtcttt ttgtttttta
    23521 caaaaaatta aaatagtggc aacaattgag gatgtgaggg agagttgcca ctattttgta
    23581 tagggtatta tattatgttt ttaagctagt tttactatct tctatgttta tataataact
    23641 cttgtgtgtg aatttaattg ataattcata tgaaaattat gtgaagtttt gatttatttg
    23701 taatatattc tactactatt ataatttttc tactggatat actaatcatt tgtttaacat
    23761 tatctgagat tgatttgcaa acttttatat aactaaatta gttatataac ttatagattc
    23821 cattaaatac cctaataatc atatatgtac atacaaagtc atgcttatgc aaaatctgca
    23881 gtttcaacta acttattata taaaccatct ttatgtagaa gttcagcatc atttccagat
    23941 tctattactc tacctttatc taatacatat atattatcag cactcttaat tgttgaaagt
    24001 ctatgagcta ttataataac tatcttatct tgtatgtact ctttcaagtt gttcaatatc
    24061 tctttttgtg aaatattatc tagagctgaa gttgcttcgt ccagaataag taccttttta
    24121 ttacttacta aagctctagc tattgatata cgttgttttt gacctcctga tattttgaca
    24181 cctttttctc ctactacttc gtccaatccc ttatctagat tttttataaa atcatctaaa
    24241 caagtaactt ctaatatctc atctaattct ttatcactta ttttattatt taatactata
    24301 ttatttttta ttgtgtcatc aaatacaaat tgatcttgag ttactatatc aatattattt
    24361 cttagtgatt ttaaattaat atcctttata ttaacaccat ctattaatat atcaccttca
    24421 tttatatccc aaagtcggta tattagattt gttattgttg atttgccaca tccacttcca
    24481 ccaacaattc ctgttacttc ccctttctca aattgtaaat ttatatcatt taaaacaagg
    24541 tttaaatcct caccatatct aaaagataca tttttaaaat ctattttctt aatttcactg
    24601 ttatctattc tatatccatt attatcaact ttaatatcta tcttttcatc tattactgaa
    24661 aatactctat tgatagacac ttttgattgt gctattctag tattcgccct tactatagct
    24721 atacatggtc caattaacat tcctgtatat tgttgaaatg ctattaactc ccccatcgtc
    24781 atcttattat ttattatttt atatccccca attccatata ttaatattgt tatcaatcct
    24841 gttagtattt gagatattgc tatattacta ctcataatga tgttcaaatt caaatctttt
    24901 tttatcaaat ctctctctct ctttaaatac ttattaaaga aattcttttt tgaatttaat
    24961 ataacaatat ccataatatt agatacgtac tcttgaatca aatttgataa actgcctgaa
    25021 tcacttctta tttcagaaat tttcttagat ataagttttg tcattttatt ttgagaaaat
    25081 gttaatatca actgtaaaaa taaaactact aaaaataaat caaactgcaa attaattaag
    25141 aaaattaacg atattatagc tgtaaacaca tttacaataa tatcaaatat aatctctgct
    25201 ccaaagtttt ctatcataaa tatatcactt tccataatgg ataaaatatt acctgttttc
    25261 ttacttgaat aatatctacc agaaagtttt gatatatggt taagcaactt tacttttaga
    25321 tttatagtaa ctttattttt tattgaagta tataaatatg ataatataac atcaaatgta
    25381 gctgataata tccttataaa aatatatatc aatattaaat ttataagcag aaaaatattt
    25441 ttatttccta ttccatcatc tattatacat ctaattagtt gaggtattac aatatttacg
    25501 ccagcaatta taaaaaccat tataaatgat actatcaact tagtcttatt cttatatact
    25561 attatattta gtattctaaa taaattatta ttaatagttt ttttcatcta tttttccatc
    25621 ttttatagtt ataattctat cagctgtctt tgcaatatta tcattatgag ttatcataac
    25681 tagtgtttgt ttgtactctt taacacacca ttcaagcata ttcataactt catttgtagt
    25741 cttactatct aagttgccag taggttcatc tgcaaatatt actgacggtc tattggctaa
    25801 agctctagct attgcaactc tttgttgttg acctcctgat agttcattcg gatatttctt
    25861 tatttgtttt tcaatcccca acttattaat taatctcata acatattctt tatcttcttt
    25921 tttcccatca agcaacacag gaagtactat attatcatat acatttataa ctggtattaa
    25981 attgtaactt tgaaatatga agccaaattc ctgtcttctt atttcagaca gcttatcatc
    26041 atttaatgta tataaatcta catcatctaa gtacacattc cctaaagttg gcttgtctaa
    26101 gcctgcaaca cagtgaagta gtgtactttt accagaacca cttgcaccta caatagctgt
    26161 aaatttatgt ggctctatat ctaaatcaat accatcaaga gctctaactt cattctcata
    26221 ttttccatat atttttttta aatcttttac cttaagtatt ttcataacgt atctccattc
    26281 ccatttaaaa aacatatatt tttaaattaa attttcgtaa ataagatcta ctgccatatc
    26341 taaattctct aattgatata agtatacttc tttgtagtca atactaatat taaactcttt
    26401 atttaaaata gttaaaaaat tatatgcatt ataatttcca atacatgtaa aattgttaaa
    26461 taaagaatat tcacttacaa attcaaagtc tatttttatt ttagaaaagt ttctatttat
    26521 cacatttatt attttccttg ctatatccaa tcttatcaaa ttagatttat aacttggagt
    26581 gttcaagttt tctattttta aatcttcttt acttttgctt ttttgaataa gtatttcttc
    26641 caattcatta aacttaatat atggattgtt ttttaatata tttgcaacaa tgccagttgc
    26701 cactgcacaa gccttactat ttttaccaaa atgagttact ttatctttaa atttaataaa
    26761 attatctttt gaatttgcat acatttgaat cttattttct ggttcgtata tatattgatt
    26821 atttttatat atattttcat tacctactac accaataaca ctttcaaata tagtaggaat
    26881 actttcttta tcttcatatt tattatgttt tgaacaaata ataaatctac cttgactatt
    26941 taaaatatta caaacctttt ctaattcttt ttcatacata ctattaatag atgcagctga
    27001 aatgtttatt atctgtatat ccgacatagt caacttgctc agtgtactaa gaagatttaa
    27061 agaacttgtt tttccagaat cattaaatat attaatagga taaatttggg tatttttact
    27121 tatatttaat atggttttcg atacaaaagt tccatgtcca tttatgtcat ttatttcctt
    27181 cccccccaaa tctttcaatt gaaaatttga atcataaaca taatttccat taatatcatc
    27241 atcatttaca tctaatcctg tgtctaagac tgctattctt actttagtca attaataatc
    27301 acctctaaaa taaattacct tgaatataaa actaatcctt accataatat aggttccaag
    27361 atgtttttca tttttttaca atttgcatca aacatttttt tgttattttt taaagtatat
    27421 attttcgatg gacaagttgt acaaaataaa tttactttac attctttaca actctcaata
    27481 tttactggtt ttaatttatc aaatatatta aatatatcca tttctctatt aagaatcctc
    27541 tctaatatgt tttcattaaa ttctaatata ttgaatatct ttaaatcctt atactctaag
    27601 tttggacacg gatatacatc tcctttatga tctatactta tctgattaac acctgcttta
    27661 cacatatttg aacataaact actttcatcc acataatcat ctttgcaaat gtaatcaata
    27721 tcaagtttat cattaagata tttgctatag ttctcattac ctctaccaat attcataaaa
    27781 tttcttatta taggcgttgt acctaaatct tcatttaatt tattgaattt ctctatgtct
    27841 ttctcattat tctttcctac caccatagac aaacctacgt tctcgaattt ttttagtttt
    27901 ataagtctaa tcacatttaa tactttacag aatactcctt tacctcttac ttcactacaa
    27961 cttaattcat cataaccatc taaactaatt tctattgcat ataaaccatt aattatatca
    28021 tcaatctgtt tttctttaat taaagttgca tttgtagaca gaattatttt acctttaaat
    28081 ttttctttta tataatccaa cagctctaag aaatcatttc tcatcaatgg ctctccacct
    28141 gtaatggtta atatccttgg atttaattta actatattat ctattacttt tcttacttga
    28201 gatgtatcta aataatcgtt attatctatt ttagaatcta cacagcaata atcacaactt
    28261 agattacatc tattagttgg agaaaatgaa attttgttta ttctctctag tctaaattca
    28321 cttagaatag aatcacttag aagtcctata tgctctaact tatctataac tcgttcataa
    28381 tattttttat cttcattttc ttggaataca tcatacaaat taccaagttg taatgcatta
    28441 tctactgaat actttagaac ttcataacat tctattggaa ttttaaccca tcttccattt
    28501 actttattac taagaataac ttgattatta tttattattt tttcacatat acaatctaaa
    28561 aaatatttgt ttgagctatt catttatttt atttccttcc tatatattta aattagaaat
    28621 ttcattaatt ttcttttata ccacttacta tactaagttt atttatcatt ttattagaaa
    28681 aataaactgc aatgacacaa acaaaaacag atatagatcc aaatattaga atttcatata
    28741 ttggaatttc aaatttaata gagttattta taccatatac ctgttgatta agtttgttag
    28801 tttcagaaac tattttataa taattgaaac tagatataat tccagctaca atcatactta
    28861 ataccccata tatcatattt tcttttatta acattctttt tacatttttt ttggacatcc
    28921 ctattgctct aaatgtacat atctcactca acctcacaat tatatttgat ctaactataa
    28981 aaataatgtt tatagatgat attaataaaa ctccaaatac acaaatcatt gctaatttat
    29041 atgaggattt attataatgg tctccaaaat gattacgtac atatcttccc tctatatctg
    29101 aaaatccact tttttctatt atattattta tttcttttat tactttttta tctgtttctt
    29161 tttttatctt cacaaaaact ttattataat cacttatccc tgttaattct tttaaatcct
    29221 tttcattaaa tataatttga attccacctg attgagattc tccatcttgc tctgatatat
    29281 agctattttt cattattcca gcgattctta cttttgtatt tatatattta tactctccat
    29341 ctttatatac aggaattttt atatctatta agtctcctat tttaggtgat tttataatct
    29401 gagtattatt tgacaattta attctagaat aaaaattatt taccaataaa acatttttat
    29461 aatccctgtc tgtataacta taaacatttt cgcctttatc tatatattta tctatattat
    29521 ttaatagctc tttattgtaa cctcgaatta aaactgtaga ctcattatta ttatttattt
    29581 cagttctttt tagctcatct aaataatctt cttctaagtc agcatttctt agatatcctt
    29641 ttaaataaat attgggttca acatctgtta catcttcaat agcttttatt tcatttaata
    29701 aagaattatc atatttagaa aatgtataat ttgcatttat tggtgaatat cccaaataaa
    29761 tatcaaaatt gccatatgat ttacttgtta tattagctga atctgactct tttccatata
    29821 tgaattttgt agaagttagt ctgcctataa ataaaacccc acatagtgat atcgtaatta
    29881 ttattattat tgatgtaacc ttattcctaa atatattttt aaaagctatc tcatttataa
    29941 ttccaacaac ttttttatgt gattttaaca acaaaagcct aggaatataa tttaacttat
    30001 ctgtcttttt tatagcatca attatagata aatttccagc tttatatatt ggtactacag
    30061 aggctaatat aactgctata attgacatga tgatagattc aattatagag tttgtattta
    30121 tagttaattc tgcactttta tcatatacta tcatgagacc taagtaagaa aaaactatac
    30181 ctccaagtaa accaattact atacctacag ataaaactac aatactctga ataaaaaata
    30241 cctgtctaat atttctcttg ctagctccaa cagctctaag taaaccaatt tgcttggtca
    30301 tatctagtat tattatgtta aacatattgt atattactaa tgttgcacat aataaaggga
    30361 gtaagctttg ctttattgaa taagttgttt tttttgaaat atttttcata tacatagaca
    30421 tacttacttc tgtatttgga taaaattgat tacttgttaa cttatatttt ctccctagat
    30481 tcactacttt ttgagaatct aaatcttttg tattcaattt taatgtaccc atatgagtta
    30541 ttaattcata tggaacaata tctttgccat tgtttgaata tgtaaaagct cttaccttat
    30601 atttatttaa attttcatag tattcatcag atttacttaa aataccaact attttaaatt
    30661 gcttatcttt actataaata gaattttcat tatttttatt tatatgctca ttaacaatct
    30721 gaaaatcaat agtttgattt aatttgacat ctaattccat tttgtctaaa actttttgtt
    30781 ctattactaa ttcattagaa gttctaggct ctctaccttt aactaactta tatctagtag
    30841 aatctaaaaa atctttattg taagagcata aatttagaga tgctccatta tctttactta
    30901 taatttctcc aaatttaagt acatcattga attcttttat atcttcttca ccattaagtt
    30961 cttttactat atcactattt atatcttcat aaaaagcatg ataatctccc gagtttttgt
    31021 atgcatgatt tatatctgat tgtccttgag attcattaat aacacttaat gaaaaaacta
    31081 gcataacagc aatccctata ctaagtatta atgataaagt ttttcctttt tgtctcttta
    31141 aatatgacac cgcaagttta atatttatca taatcagaat cctccttatt catatttaca
    31201 agaaaaactc tataacctaa aatagtaggt tatagagcac tattcattaa atactaacta
    31261 actcgttgtg ctagttaaat ttaattatta aaaatactag agttgagaaa atcacatttc
    31321 atttgtaaaa aactacatct agtactttca acatatttaa tagactgatt agtatttttt
    31381 aataagctac tgttcctttt ggagttgatc ctgcacaatc caccatacag tctatttgtg
    31441 aaattattcc atgcttatca taataccatt tacatggatc tgcttgcacc tcaacatctt
    31501 gaagttctat tttttcttcg atttttttta tatgtatcat aatacatatc cccctttact
    31561 tttgttttat atttattata actagttaaa ctagattata aactacatag tttttttgtc
    31621 gtttccacat aaactctttc taatccacaa taaattggta ctgcactttg gaatttacct
    31681 gttacctttt tattgataag tttacatctg gttgacatac aataatcaag tctagtgcat
    31741 ccatcacact cttcattctt agatatataa atttttataa ggttcttaat ttttttatca
    31801 tctaatccat catatatatt tcctagtaaa atatctttat ctcctacaca tactgtacat
    31861 ggatatacat ctcctctagt atctattgca taactattta tccctccaaa acaatctcct
    31921 tttttcttat ttataaattg tttatctaaa atagatatat ctatctcagg ataatccttt
    31981 ttaatattca taattttact aatttgttca tttaatattt taatatgaga attatcccaa
    32041 tttttatcca taaaatctga aattggtgcg atatttttaa acccttgttt tgctatatca
    32101 tatacactct cataaagata cttaacagta tttgagttat atgtcattct tgccctaaca
    32161 tatatacctt tatctataag tctttttcca ttttccatta ctttttcata agtacctact
    32221 ccgttagcat ctattctatt tatatcatgt atattctttt ttccatcaat acttatcgat
    32281 atgtttttta cattctttgc tataaactct attatttcat catttataat agttccattt
    32341 gtagttatat caaataatat ctttttgtca ctcaatctat ctactaaatc cctatgtata
    32401 tatttaatca aatcaaaatt tataaatggt tctcctccat gtaaaacaat tgttgtataa
    32461 tcactattac tttctaattc tttattacaa ataaaatcta ttgttttatc agcagtttca
    32521 taactcatat tttctttttt tttatcatta ccttcataac aatatgtaca tctcaaattg
    32581 cagtcttcag ttacaaatat agtaaatttc aattaacttt ttcatctccc catacagctg
    32641 tatttaaatt gtgtttcatc atactacacc atctatccaa ttccaattca ttatccttaa
    32701 ctgaatctaa tatcgctggg caatgccaac aaaaaatatt tacatcacaa tctttacatt
    32761 tttcaaagtt atatggataa atactttcta atttaatcaa agactttaga tctcttgttt
    32821 tattttcttt tataactttt acaaattcat tgtcaaatat atttcccatt ttatagtcat
    32881 cctttatcag tgaaggacaa ggatatacat ctccatcata atttataaat atttgagagt
    32941 ttcctgcatt acatgcacaa catcccattt ttttattttt tttatcttta cacattttta
    33001 attcattatc taaaatatac ggaattgtaa ttggaagaac tgtatcttca attaaatagc
    33061 tttctttatt ttctaggcct cttcctgttg gaataaaata tctttcaaca aattctgttc
    33121 caagcttttt atttaaatct ttaaattgtt tatttaaatg agaatttttt tgtccaaata
    33181 ccatagacag ctttatatta ctgaatccat tgtcttttag cagattaaca gagcttatta
    33241 ctttattaaa tacacctttt cctcgtgttt cactacaagt attttcatct actccatcaa
    33301 tactaatatc tattttttca aaattagtta taataaaatc aacattatgt tcatctatta
    33361 aagttccatt tgtggataat attaatttgg tatcaggtaa tttttgtttt atatatttaa
    33421 ttatttcaaa aaaatcttct cttatcaatg gttctccacc agttataatt atatgaggag
    33481 tatttaaaac tgatattata tctacgatat tttttaaatc atatgtacta agtaaatctt
    33541 tcttattaga agaagattca acacaacaat gtttacaaaa taaattgcat ctatttgtca
    33601 aacaaaaata aattgcaaca ccattgctta acatatcaaa gtttaaattg ttacttttaa
    33661 aaatttgatt
//
LOCUS       DAGSRM010000036        32883 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.036, whole genome shotgun sequence.
ACCESSION   DAGSRM010000036 DAGSRM010000000
VERSION     DAGSRM010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32883
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.036"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..529)
                     /locus_tag="MJE33_001881"
     CDS             complement(<1..529)
                     /locus_tag="MJE33_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HBY9647817.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRVMLENEDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEVDEILRLKQITGLAYLFKD
                     KEFGNAWIPFGSDIEE"
     gene            complement(547..1860)
                     /locus_tag="MJE33_001882"
     CDS             complement(547..1860)
                     /locus_tag="MJE33_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HBY9647818.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEDNIEDIEPYWVCLPMATRTAV
                     GNYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRAAESLQRYCSIVNKQHGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGVAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVKLPYGHTWKSFTYFLLDTLPENIRNSYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
     gene            2215..2655
                     /locus_tag="MJE33_001883"
     CDS             2215..2655
                     /locus_tag="MJE33_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBY9647819.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKDGYARLCKLETYLKTQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYAFFEKGAFTVLIQIGDKQVPAVEKTLVALTPKA
                     KELWVTRYPCGNNGGWIKYRILEDADLLDIIQYIRAKKSPPKQN"
     gene            complement(2840..3028)
                     /locus_tag="MJE33_001884"
     CDS             complement(2840..3028)
                     /locus_tag="MJE33_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647820.1"
                     /translation="MNKNNNLVIICMFIGMILGMAIGCAIGISKGNVGITMCYGLIFG
                     MIIGICIGTVIKNSNKKE"
     gene            3969..4364
                     /locus_tag="MJE33_001885"
     CDS             3969..4364
                     /locus_tag="MJE33_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647821.1"
                     /translation="MNILKMKKTGIFLGGVLFGTVGVKILSSDCVKKTCTRATAGALK
                     VKDSVLETTTKIQENVEDILAEAKEINEQEDLSTSKVNVVQDTLKCEQEVLKTETIKT
                     VEDTNSNSIKCEEIKDNVIEEKNSTCTTE"
     gene            4407..6506
                     /locus_tag="MJE33_001886"
     CDS             4407..6506
                     /locus_tag="MJE33_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBY9647822.1"
                     /translation="MNFYIKHEIRGRIRFDLQLGKLNNKQSDTLLYYLTSINGITKAK
                     VYDRTGDAVIYYTGEREYIIREITKFSFNNSKIENLVPEHTSRELNNYYRDKLANKLL
                     VKSISKIFFPKSLRFFLIGLHSIKYFKKGLSSLLNKKIEVSVLDATAIGISIFRKDIN
                     TAGSVMFLLGIGELLEEWTRKKSIDDLAQSMSLNIEKVWLRKGDTEILIPISEIKEGD
                     LVSVTMGNMIPLDGVIVSGETMVNQASLTGESLAVNKKEGSYIYAGTVIEQGNIVMCV
                     KEKAGTTRFQKIVTMIEESEKLKSSVESKFEHLADTLVPYSFLGSILTYAITRNPIKS
                     LSILMVDFSCALKLSIPISVLSAMRECNNNNITVKGGKFLEGVACADTIVFDKTGTLT
                     KAQPTVSDIITFQNYNKEDMLRLAACLEEHFPHSIANAVVYEAEKQGLSHKEMHTEVE
                     YIIAHGISTKVDDKKVVIGSKHFVFEDEKCTIPDGEEDKYNNLSDEYSHLFMAISGKL
                     SAVICINDPLRKEAKYVISNLRECGIKKIVMMTGDSEKTAKSIASKVGVDEYYSEVLP
                     EDKANFVKSEKLKGRKVIMIGDGINDSPAISESDVGIAMSEGAEIAREISDITISADN
                     LNNLIVLKQISNKLMKRVDLSSKFIIGFNLGLILLGVGGFVRPSTSAFLHNASTVGIS
                     LNSMTNLLENTNTYNYH"
     gene            complement(6731..11941)
                     /locus_tag="MJE33_001887"
     CDS             complement(6731..11941)
                     /locus_tag="MJE33_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HBY9647823.1"
                     /translation="MANKLYSEIVNLLEEGRDELRKYDLKEKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDEETGQIYITAKDSAVEIGNGVYSKTLCPEVVDIANRDFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRHTLLIETGKNLKDM
                     LGDYAKYKENFFFLINKIPKALIGNVTEEKIRNILKKAKEDLDSQEKDYDGYKELVYL
                     LNVALSDGVSIKLCNPLDKQPEKLLNEMKNLPETFEKTDLGLMLTASCKIEIQKAVDV
                     TYKDLEKLLVPYIESVKKGMEDSISILSSINSLKDKKIQLDKMYTDLCSRTNNMKELM
                     EFVQELSYGDGVQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLKDI
                     KYWIAEEEYREEEAKKLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLTK
                     IISGYQLNILSSIYMYGDCELKIDCNMEKEVFSGKNIVLLTEKLHIPEGTYSINVSGK
                     DAEKLVGESSIYDGMNGRNGNNAGTSGDIILSFKEIEKRGELKLYVDGGRGGNGQDGK
                     KGDTGITGEAGANAKIGSKKESKEFANVKGTKYYVVKAEKGKQGGTGGTGGNGGNAGL
                     GSKSGQVYLLGKAPLLKSAISKKDGNHGLAGKGGSGGAGGAGGLGGIRGIYEETIRYI
                     GTSPPMDNSDRNYYYFDRYVFNHALNQKQAADDGEKLNTLYEEHKDWTISKREDSGSA
                     GAEGKAGHSGDALKEKELLKQEYSLSIDTFFYEFFERFGFSGIKGYRNLRLLCAVGIL
                     EADILELSHIYEIFHAVEKIEMQLNREILQKYYSFEKKNKAVLSLDDMNTKKEFYKEA
                     KEIYEYLINKLMQSDRIESFVGKNQNFSEWSMILFALEQKTAFYVQMIEESEIGGNRV
                     TDIRSLTDYLDKTLRELEQGKKDELKEGYFNTYKESILTQIESGNQQIEVLKNELENK
                     MLSVNAKMNLLIQEIIKMKEDEQEDIEELKKKQAKLEEQMKTQLILSGIQSALSLLTA
                     FSGVGQACLGAFDSAKSLKENLSSKEEEYKWTNRMVNFSDFKLDSDGMRFVLGEQLSE
                     DRKNALDKMTYNLAGVDEERRTKFDQIKEIIVKSIDDDNYADKADDIQRAKEGLSESE
                     YKEIYTKIQKKEIGMLEANYNNKQQKDKLAALEKSKQAIERQQKTSKIKNALSIGSSA
                     CSLINTAISGVNNCLNVKSSFESKIEVVKEAIKDKENNIKKLDELNTQMVEFKDKTLK
                     ESVIPFVEKFDSTVKNQDIFENEISKLKMKEVLTELSTKFGSLKMENSEEISIIFKKV
                     QDMMETQIIITDKIDKKKDNLAMGELIYQMSDSGNQMNEYQKKLIHQIELNQVRYLCQ
                     VEAAAFQLWTFPFGSNIVNILNRCHVGITEDENEVINQAKEQNRQIREFLDQDAYQWQ
                     KTDNHILKVALTGSLDGKSRVGFENTYSLYDCNEEEVKKLLTGDEISFEVPLNKNFDA
                     IKYVTMYANIPNLRRNILEDSLKVKVHIHLYDSGYFSLYDDKKDVYYQYVFPQNYINI
                     THGLDVKYNDSDSINSIWSSVEDKGIEKFKNAGLNSGRSPYSMAKISLKAHFNRKLSY
                     DSLTNEQKKKLESYFDKPSKYAWSDSIDILRSLGYIDLKGILSKSWYEIKKDIDSDGE
                     IMKHFSILQVDLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYE
                     ERLVENTIIG"
     gene            complement(11956..14451)
                     /locus_tag="MJE33_001888"
     CDS             complement(11956..14451)
                     /locus_tag="MJE33_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBY9647824.1"
                     /translation="MPENNNQLLEEITLLLNEGKVAINKAENQNLILILGGTGCGKST
                     AINYLAGCVMMEVEDEDTGISYIDCLDPVAEIGSGAVSQTLYPNIVNMRKPIEGMDAK
                     LCDTAGFSDNRGVAHDISAAVSLGEVFKNSASICAVIVLIPEADILDSRLTKVLDLFQ
                     QLNMCLHKENFKGSITFFISKSFLGRTEKQIYNVIKRKYEQLCENLDSNIEEKAWLFE
                     ELLKDNGKNIHLCKPLASDERDGLLKQVLELDEVKDKTSAFQYPISADARLVLDTLIK
                     EIQNNIVSSLEDYVKAYTEDFISIIGSITMIQEIAKYDEVVKVFKNWLESTTSLTIDR
                     FINVLAKQSGLFLQDSCMKLQNKIKGNLDIIDALSKYTEHGTGEILLPSVHMEFIIEK
                     ASKKIMEQNFIIIRKNLEKVLVSYEVQKQVFTLPNREKLMEVANKKDYVYSVSDFNSI
                     LTNVTVTQNEELEDSITRLSAYGEEGNEIIREFIGKYILNPFIVKTININDKKGIEIR
                     ALIPNLVISSVLEYFKNRKVDDNVSTFMFFAKDTIYMDDNLGMDFASEKNIILACNNL
                     DVGSNVKINVSGKSGELVDSSMYGKNGEDGRDSGNIYLYIKEEIRNYRLTLIANGGNG
                     SKGIDGKNGETGASGSNGADAGYTSYFHGQMFKGYFAKGTSGKKGEKGGAGGNAGRGG
                     NAGKKGNIYFISDSEEQKNRIINHCDITVKDGEAGPNGRPGSGGYGGSGGYHGYNTLS
                     FSPSGIDKTVYYTGYFTSWDYKLDSIIKYHIEFSGGSWNEGKTGPAREYAEYGDRGDN
                     GGFNAPNTKLSTAVSKVEENLIKEKYQGYCSEI"
     gene            complement(15088..15375)
                     /locus_tag="MJE33_001889"
     CDS             complement(15088..15375)
                     /locus_tag="MJE33_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647825.1"
                     /translation="MRLNVTKQNTIEVENSRKEDVYFVIVDNVFFERQGDVFNSITNN
                     NLNNYDVKILPTDNVFLELKITSTKDLEPVFIVVEDRQYNHGSIMFKPVLV"
     gene            complement(15395..15670)
                     /locus_tag="MJE33_001890"
     CDS             complement(15395..15670)
                     /locus_tag="MJE33_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647826.1"
                     /translation="MSLFVGGWSKFRAVEEEDLKVFNEAVGMLEGVGYEPLIVSTQVV
                     AGTNFKFICNATSVTNPPQNYLAEIVVFKPLPCDEHNATITSINIIK"
     gene            complement(16303..16482)
                     /locus_tag="MJE33_001891"
     CDS             complement(16303..16482)
                     /locus_tag="MJE33_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647827.1"
                     /translation="MGKDRFEKVYSQGKINATEIWVDKETGVNYVFHYYGNAAGFTPL
                     LDREGKPVISPIVSK"
     gene            complement(16767..18233)
                     /locus_tag="MJE33_001892"
     CDS             complement(16767..18233)
                     /locus_tag="MJE33_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY9647828.1"
                     /translation="MKRKPQYLIIFEHIIQQIYTGKITIGDRLDSIKSMSEEYMVSKN
                     TIKTVIKMLSEKGFIETKAGSRPVLIQSTSKQSIESDLLYEKILQISEIYKIFSLIFP
                     SIAMYNIKRFTSKDFEELNEIIDCMEKNSSNYLVFKEQRLKYINKLISKTNNFLIHHF
                     CEIIQHDVIISQLAYNFDNLDNCCIDFFRNKYIEQVRKTYLYGLDGNLIEFKKLLTYM
                     YKACRESILKIVENRAPQSIDTSSFNSVTLEKCYLYDLIVSDVICQIFEGNIKIGDCL
                     PSITSIINRYNVSLPTVRSAYNELNELGIAKTINGKGTYVTLFSEYDDDYINTEEGAK
                     RLSLLLDAMEFVTITLEDVVLLLGNKMDLIAVDKIETHLRYLQNNCEECKVYPDFVLL
                     CKIVDYTQIYVLQETFLHLKQYMIFGIYLERFFHEEYNEILKTRFDVCFEILRYLKKG
                     DVERFAESFSNIFKESLQYLKKVISSLNVDMRVKLDIL"
     gene            19436..20614
                     /locus_tag="MJE33_001893"
     CDS             19436..20614
                     /locus_tag="MJE33_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBY9647829.1"
                     /translation="MKYHIFLIITTLLIIELCNIYICKIASFGNKNITIHKNYKYIFL
                     IKPLTFLIIIFTKLPLPLYYILFYIVSHKLLLSRKSSTNNHFIPNMAVISFCILHLIT
                     VSTISLVKNTSPDIIFANMTYKLISILIASILDFILWYSMNKIKEHIYIITQNQIEFS
                     LFLKFIWFTLGYILFDSIQITFAPPSSLTSYFILGSNILLILQLIIFINHIYSIEKNI
                     YLEKEHHRLKMIKQEQLLKVNLLKKKANIDELTSAYNRKFAFEKIQHYIDNNLLFSLI
                     YIDLDKLKTINDTYGHLAGDNYIINFSSIVIENLGIDDIFARIGGDEFIVIMPKSNEN
                     TTKEFIEKISKIMDNKISFSYGIVEVSSENKMTVEEIINIADIRMYTNKQNNHKRNNN
                     "
     gene            20964..21845
                     /locus_tag="MJE33_001894"
     CDS             20964..21845
                     /locus_tag="MJE33_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBY9647830.1"
                     /translation="MPAYLFDSNSITNIYRVFSILLSFIVTGLYFSYKNHKNNKINFE
                     AVLEDKKNLIFLLSLMFGMYLYLCLSLSIYYTVGNVLILKLYGIKSAIIVLVGNYIAE
                     RYSFRITQIKGYDIKNQIEYVESVKTEYEINKLNDIVYTDLLTGFYNRPFVNQKLSEW
                     TSNHYKFTLCFTDLNSLKPVNDKFGHQFGDKYISTTAQIIKKLINVENSLLFRYGGDE
                     FLFLLQNTSCVEAEKIMININYELSKLSNTDEYPYALSISYGLVEWDGISDIETLISK
                     ADSLMYENKLKNKSTLN"
     gene            22054..22203
                     /locus_tag="MJE33_001895"
     CDS             22054..22203
                     /locus_tag="MJE33_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008819224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBY9647831.1"
                     /translation="MTTVAEGIETSFQKDFLQEIDCDMLQGYVFSRPLPIKDFEKLMF
                     QNSSN"
     gene            complement(22463..25030)
                     /locus_tag="MJE33_001896"
     CDS             complement(22463..25030)
                     /locus_tag="MJE33_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBY9647832.1"
                     /translation="MIYYVLPLEHKQATIEIVGGKGMSLSKLLTAGIPVPDGFHVTTA
                     SYQIFVETNHIQSRINKLLDGIDSNNTSQLEDVSKKIGELFHNGEMPQEVSDAIKTAY
                     AGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDKVIDAVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYALTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSKP
                     LLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPLHTMNAHQIMEGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIVLQSEKA
                     LWGISEWVKQNNSINLYLQNSSAIKIAEDFMSSVVPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAHSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQKISERFIRGGAISHLDD
                     IYWLTKSELEVLIEQLDKNKSLSDMNGIIPVRKAELKKYMGYVSPSKLPEKNKKTISQ
                     EPQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRTIKSGQMITVDGLAGTVTFDE"
     gene            complement(25033..25638)
                     /locus_tag="MJE33_001897"
     CDS             complement(25033..25638)
                     /locus_tag="MJE33_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY9647833.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDGEPSPQQCLRKIMLNHLRFP
                     EQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTDTALVT
                     RLLINSMLSISYTSRPSDSSEKMLDETMNIFLHGIMNDGGE"
     gene            complement(26012..27472)
                     /locus_tag="MJE33_001898"
     CDS             complement(26012..27472)
                     /locus_tag="MJE33_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HBY9647834.1"
                     /translation="MFNELKTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEVIKNTKESIKVWAEMPVYKRANIFHKAADLLLENIDEIANVLVLEIAKD
                     IKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVGKIKPLVEKLTIGNPMDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLITGNVRKNNLFYPTLLDNVNVDMKIAWEEPFGPVL
                     PIIRVKDINQAIEIANQSEYGLQSSVFTSDIDKAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVREL"
     gene            27827..28405
                     /locus_tag="MJE33_001899"
     CDS             27827..28405
                     /locus_tag="MJE33_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY9647835.1"
                     /translation="MAKSFTEIEKDNIRKRLISECELNWSKYGYKKTNIDELCSKSGI
                     AKGSFYTFFDSKEKLFFEVLNGIQNRLISSVDEILSNMPPKEGFAKSLKMLYRLYDKN
                     PFLYSPNNPDFISLINKLPQDMFLQLESNNIQSFYDLIERHNLKLKIEKEKVFGVCNA
                     LLSSLLVKENIEYNYIEVFDFMVDNLVEHILE"
     gene            28696..29946
                     /locus_tag="MJE33_001900"
     CDS             28696..29946
                     /locus_tag="MJE33_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY9647836.1"
                     /translation="MVKSSNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIVFSPIGGAIADIFNRRNLMVIFDFSSSLTVLILAVFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAGPVLGG
                     VLYGVIGINAIIVISCVSFFLSAIMEIFIQIPFIKQEQNSNIIKTIFTDIKDGIVHIC
                     KENRFILKILFLAIAINLFLSSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKHLKITNLHISFIICAVMFIPMAVSVYPFILNTGFPVPFLIFIVSSMLIFF
                     TTTLISIYVITVTQLKTPNELLGKVMSILFAGTAIAMPLGQVMYGKLFDLLSDRVYII
                     ILVVGFITLLIAFVSKITLRNENL"
     gene            30107..30676
                     /locus_tag="MJE33_001901"
     CDS             30107..30676
                     /locus_tag="MJE33_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBY9647837.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVEFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAAAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
     gene            complement(31298..32638)
                     /locus_tag="MJE33_001902"
     CDS             complement(31298..32638)
                     /locus_tag="MJE33_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBY9647838.1"
                     /translation="MIKSSLFWNIFIAISFMIEWFLCKKMLDYTSEKRISNKKINFCM
                     VGIIVFADIIYFFDVPPNIRVIISMIITIAFYKISYDVGFIKSVLISLIYWMLLIATD
                     ALSISLTVWLNSINDMSSMGTGDIYRIESIILGKILLIVSLLLYRALKIKLNIEINKR
                     TLFYIVIPIVANIASFFVIFEYIFNPGSGSLIHNFQFVMISVVLLISSITLVLIAKKL
                     QQYNTLVVENNMIKNTLKYENEYYEDIEKKYLKTRLLSHDMKNHILCINAMIKKGIDV
                     TSYLNNLQVEIQSNDLLFNTGNFILDAILSEKKEVCDKNDIELKIGINFSCCEAVELV
                     DICHIFSNILDNAIEACNKIDSGNRVICVKGDVMQNFYLIRVENTKMNKIITKNGDIL
                     TDKKGSLHGLGIKSVKNSVNKYGGNVVIEYDDKKFVVKIAIPLRNVKKAVNHVI"
BASE COUNT        11165 a         5603 c         3869 g        12246 t
ORIGIN      
        1 cttcttctat atcagagcca aagggaatcc atgcattacc aaactcttta tctttaaata
       61 aataagctaa tccagttatt tgcttcaatc taagaatttc atcaacttcc atacccaggt
      121 gctttgatat ccattcatca gattttccaa tttcacaaag ttcagttata attttactca
      181 ttaaatccac attatgagaa cctcttgcac gattgtgtcg aatagtactt gccattcttt
      241 catcaagaga tttatcaatt actgaaacag gaagaagtcc gttttctcta tcatatatat
      301 cctcattttc aagcattact ctatatctat gaaaaccatc tacaataata tacttatcct
      361 tttcctcatt ataataacag acaattggca ttgtataccc atcttctttt atacttttat
      421 acaacaactg catttctggt ggggctactg catttggatt atagtcgtta gattctatct
      481 tttcgattgg cactgcgatg attccatata caggactttt atattccata gcatcttaac
      541 tcctatctaa tgaatttttt catatttctt cttaatatat tccgttctaa ttctctgttc
      601 ttttgtagga ccaaatccca taaatctaca catatggtca ttttttaaaa tacaataaca
      661 cattcttttc caacttggta tatctttggt aaatttaata tcatccgtat catcaggtat
      721 atcatccaaa aatatgactc tactcttttt atcttttgta tgatttgaaa ctccatttaa
      781 ttctatttta taaccttttc tcaacaattc acgtataact atttctgaca aaccaccacc
      841 tgtattctgc caaaactcaa tagatgttga aaatttttta atatagctat tccttatatt
      901 ttcaggtaag gtatcaagca aaaaatatgt gaaacttttc catgtgtgtc catatggcaa
      961 tttaacacta cgatatccca tagcttttgt tttgccataa atagaagtaa aattaactcc
     1021 ttgtactcta gcaaccaatt tcacccatac atctgggtcc aatattctgt ataaatttaa
     1081 actctctttt gctgaatcat taaaaggtga tgccactctc atttttcttg gagctactcc
     1141 agctttatag aataagtcat aaattttatt atagtcatag tcaaatttat agtgtgctaa
     1201 ccatatatct tctatttccc aatcatatat aggagatgcc gtccagacat ttttaaattc
     1261 cttagttatc cagcactttc ctttatatcc atgctgttta ttcacaatag aacaataacg
     1321 ttgtagagat tctgctgctc gtgttcctaa taagcaaaca acttttgcac cttcatgtat
     1381 atctctaatc catctaccaa attgttttgc caaactttct tgtctcattt tatatttata
     1441 ataagtaaaa ggattatttt tttgatttat tatgtattct ttgactggca tttcccttac
     1501 ccataactct tctttctcat catcccatgg ataccaatac atttcataat ttcctacagc
     1561 agttcttgta gccattggta aacacaccca atatggttca atatcttcaa tattatcttc
     1621 aaatactttt tcaacatatt cagtagtcaa actatattga gcctcaaaat cttgatgaaa
     1681 tactcctatt ttggaactta tattattctt tctcttataa tccaaaacta aatttagcag
     1741 taaaccacta tcttttcctc cgctaaaaga tacatagata taatcaaatt cattaaaaag
     1801 ataagaaagt ctttcttgaa aagcttcaaa tacatcggat tctaaatatc tcttatccat
     1861 ttgaattatc ccctttcaat tagaagttta taaaacctta caatgcaata ttattatgct
     1921 tttttagcag taatcagcat aaattataca ttatttaaat acacaaatcc aactaaatac
     1981 atataattat aatgtaatta tacttttgta ttataaacta tattttatca ttattttaca
     2041 tttttttcca atatatataa ttatcatatt tttctattag ctaaaactaa ttaccttata
     2101 tttttacgta ttggtataat aatacataaa aacttgaata atgcataaaa acttgacata
     2161 ctgttgtcat aattactttg ctataattaa aagtaaaaat ggaggtaaat tctaatgtat
     2221 gaacgtatgc ttgataaaag caataaaccc tgtttgtctc aaataataga atatataggt
     2281 aaagatggat atgcacgatt gtgtaagttg gaaacttact tgaaaactca atataattta
     2341 tcaagagaac ttcgatttcc ttttggaaac aattatggtt ggggttacaa atatagccat
     2401 aaatcttttc atttatttta tgcttttttt gaaaaaggtg catttactgt tctgattcag
     2461 ataggtgaca agcaagttcc agcagtagaa aaaacgttgg tagctttgac accaaaagct
     2521 aaagaactct gggtcactcg ttatccttgt ggcaataatg gaggctggat taagtatcgt
     2581 attttagaag atgcagactt acttgatatt attcaatata taagagcaaa aaaatctcca
     2641 cctaaacaaa attaactttt attttttgtg atttaaatat agtgtatata aattccattc
     2701 tttccatata aaaaaaataa tataaaaaat atctattagt ataacttatt tagtatgtct
     2761 tattctactt tttacagata attaaaaagt tttatcataa ataaatcata actagacatc
     2821 ctagtaaatc aaaattaatt tattcttttt tattagaatt tttgataaca gtccctatac
     2881 atatgccaat tatcattcca aaaattagac cataacacat tgtgatacct acatttcctt
     2941 tagaaatacc tatagcacaa cctatagcca ttccaagtat catgcctata aacatacata
     3001 taataactaa attgttgttt ttattcatag tttttctcca ttcaatatat tttaaattgc
     3061 ctagctcgcc ttcttagctt aaaacaaaga aatataaatt aactttcttt cattctaata
     3121 tctgcttgta taaatgtcaa acaacacatt tcttgtcata acctcctctt tttgaagtaa
     3181 gtacaatctc ccagacgcaa taatatcaat ttcctaaaac catttaagta acttttatat
     3241 ttacataata tttacaatca taaactatat tgttattcat ttatacatca taaacactcc
     3301 ctcctaatca ttattatact taaaataatg ttcatttata ataatatatc taacgcactt
     3361 taattgaaaa aactaaaact ttgcctttca ttcccatata taaataagta tactttttga
     3421 tgctctatta caaatcaact aaattaattg atataaatta tcattttctg ttgaaaatac
     3481 cattcgaaca tgctatactt aattgcataa atggtaataa atgtcatatg attttacaaa
     3541 tctattaata acaattatta cttttactgt attctaaagt ttttggacta aatatcaaga
     3601 actttctaaa atacaaagta caaaaaattt atataagtta taaatttata tgaactagat
     3661 aagctcaaac aatatgttat gtataaactg tgtagtattc ggttataaat ttatataaat
     3721 tatataagct agataaagta ttacctcaac tatataagct acatatataa attacataca
     3781 taagctatat aagaattatt tcaactatat aaactacgca tgtaaaattc atgtaaattt
     3841 actaatataa atcaaataaa attactaaac tctctaaact aattttgtaa aacatactct
     3901 atttttaaca tttgttacct tatacataag tacaaacaaa atctaactaa atgaaaggat
     3961 aatgtaaaat gaatatttta aaaatgaaaa aaacagggat atttttagga ggagtacttt
     4021 ttggtactgt aggtgttaag atactttcaa gtgattgtgt taaaaaaact tgcactcgtg
     4081 caacagcagg agcattgaaa gttaaagatt ctgttcttga aacaactacg aaaattcaag
     4141 aaaatgtaga agatatttta gctgaagcaa aagaaatcaa cgagcaagaa gacttatcta
     4201 catctaaggt aaacgttgta caagatactc ttaaatgcga acaagaagtt cttaaaactg
     4261 aaactataaa aactgtagaa gatactaatt ctaatagcat taaatgtgaa gaaatcaaag
     4321 ataacgtaat tgaagaaaaa aatagtactt gtacaacaga ataaatttaa tgattattat
     4381 gattaataat atcaggagga agtaaattga atttttatat aaaacacgaa ataagaggtc
     4441 gtatacgctt tgacttacaa cttggcaaat taaacaacaa gcagtccgat actctcttat
     4501 attatttaac ttctataaat ggtattacca aagcaaaagt ttacgataga actggtgatg
     4561 cagtcattta ttacactggt gaaagagaat acataataag agaaatcact aaattcagtt
     4621 ttaacaatag caaaatagaa aacctagttc ctgaacatac cagcagggaa ttaaacaatt
     4681 attatagaga caaattagca aataaactgc tagtcaaaag tatctccaaa atattttttc
     4741 caaagtcctt aagatttttt ttaattggac ttcattctat aaaatatttt aaaaaaggtc
     4801 tctcttccct gctaaataaa aaaatagaag tttctgtttt agatgccact gctataggta
     4861 tttctatttt tagaaaagat atcaatacgg ctggttcagt tatgtttctt cttggtatcg
     4921 gtgaactatt agaagaatgg actagaaaaa aatctataga cgacttggct caaagtatgt
     4981 cactaaacat tgaaaaagta tggttaagaa aaggtgacac tgaaatctta attccaattt
     5041 cagagataaa ggaaggagat ttagtaagtg taactatggg aaatatgatt ccattggatg
     5101 gagttatagt atcaggagaa acaatggtca atcaggcatc acttacaggt gaatcccttg
     5161 cagttaacaa aaaagaaggc tcttacatat atgctggaac tgtaatagag caaggcaata
     5221 ttgttatgtg cgtcaaggaa aaagctggta ctactagatt ccagaaaata gtaactatga
     5281 ttgaggaatc tgaaaaacta aaatcttctg tagagagtaa atttgaacat ctagcagata
     5341 cattagttcc atacagcttt ttaggaagta tattaaccta tgcaattact agaaacccta
     5401 taaaatcact ctctatatta atggtagatt tctcttgtgc attaaaactc tctatcccta
     5461 tttcagtact atcagccatg agagaatgta acaataacaa cattactgta aaaggtggaa
     5521 aatttcttga aggtgttgca tgtgcagata ctattgtatt tgataagaca ggcacattaa
     5581 caaaagctca accaactgtt tcagatataa ttacattcca aaattataat aaggaggata
     5641 tgttaagact tgcagcttgc ttagaagaac acttcccaca ctccatagca aatgcagtag
     5701 tttatgaagc tgaaaaacaa gggcttagtc acaaagaaat gcatacagaa gtagaatata
     5761 ttattgctca tggcatttca acaaaagttg atgataaaaa ggttgtaata ggtagcaaac
     5821 attttgtatt tgaagatgag aagtgtacta tcccagacgg tgaggaggat aagtacaata
     5881 atctatcaga tgagtattca cacctattta tggcaatatc aggtaaatta tcagcagtta
     5941 tttgcataaa tgacccttta cgaaaagaag caaaatatgt aatttcaaat ttaagagaat
     6001 gcggaataaa aaagattgtg atgatgactg gagatagcga aaaaacagct aaatctatag
     6061 caagtaaagt tggtgttgat gaatattatt cagaagtctt accagaagac aaagccaatt
     6121 ttgttaaaag tgaaaaactc aaaggtcgca aagtaattat gattggtgat ggaatcaatg
     6181 attctcctgc tatttcagaa tcagatgtcg gaattgcaat gagtgaaggt gctgaaatag
     6241 caagagagat ttcagatatt accatatcag cagataattt gaataattta atcgttttaa
     6301 agcagattag taataagctt atgaaaagag ttgatttaag ttctaagttt attatcggct
     6361 ttaatttagg tttgatttta cttggtgtag gtggatttgt tagaccaagt acatctgcct
     6421 ttttacacaa cgcatctact gtcggaataa gtttaaacag tatgacaaat ttattggaaa
     6481 acaccaatac ttataactac cattaatctc cattacataa aacatatgat ataaaaaatt
     6541 tataattcat ctttttaaaa aaatatgtct taaaattagc ttttttctaa tcaaaattat
     6601 ataaagcata ctttacaaat tttaaactca acttatttct attttttaca aaaaaggaga
     6661 tgattcaaat tttcattttg aaacatcccc ttttgtaaat ttctttaaaa tttatatatt
     6721 aattatattt tcaaccaata attgtatttt caaccaatct ttcttcatag cattttaaat
     6781 cattttgctt tagataatta aattcagact ctacataaat accacagcct accaattcaa
     6841 ttgtcaaatt aggtatattt tcctttagta tactagtctg ctcataaata attctttcca
     6901 ataaatcaac ttgtaatata gaaaaatgct tcataatctc cccatcacta tctatatctt
     6961 tcttgatttc ataccaactc tttgacaaaa ttcccttcaa atcaatatac cctagactcc
     7021 tcaatatatc tatactatcg ctccatgcat acttactagg tttatcaaag tatgactcta
     7081 gtttcttctt ctgctcattt gtaagtgaat cataagaaag cttcctatta aaatgtgcct
     7141 tcaaagaaat cttagccata gaatatggac ttctaccaga gtttaatcct gcatttttga
     7201 acttttcaat tcctttatct tctactgaag accatattga gtttattgaa tctgaatcat
     7261 tgtatttaac atcaagacca tgtgtaatat ttatatagtt ttgtggaaat acatattgat
     7321 aatagacatc ttttttatca tcatatagag agaagtatcc actatcatat aaatgaatat
     7381 gcacctttac ttttaaacta tcctctaaaa tatttcgtct aaggttcggg atatttgcat
     7441 acatggttac atattttatg gcatcaaagt ttttatttaa tggaacttca aaactaattt
     7501 catctcctgt caaaagcttt ttcacttctt cttcattaca atcatataga gaatatgtat
     7561 tttcaaatcc cacacgtgat ttcccatcta aactgcctgt taatgctacc ttcaaaatat
     7621 ggttatctgt tttttgccat tggtaagcat cttggtcaag aaactctcta atctgtctat
     7681 tttgctcttt cgcctgattt ataacctcgt tttcatcttc tgtaatccct acatggcatc
     7741 tatttaatat atttactata ttgcttccaa atgggaatgt ccatagttga aaagctgctg
     7801 cctcaacctg acacaagtat ctaacctgat ttaattcaat ttgatggata agttttttct
     7861 gatattcatt catctgatta ccactatctg acatttgata aatcaattct cccattgcaa
     7921 gattatcttt tttcttatca atcttatcag taatgataat ttgagtttcc atcatatcct
     7981 gtactttttt aaatattata gaaatctcct ctgaattttc catttttaag cttccaaact
     8041 tcgtgcttaa ttcagttaaa acttccttca tctttaattt acttatctca ttttcaaaaa
     8101 tatcttgatt tttaactgta ctatcgaatt tttctacaaa tggtataaca gattctttca
     8161 aagttttatc tttaaattcc accatttgag tattcaattc atctaatttc ttaatattat
     8221 tttctttatc tttaattgcc tctttaacta cctctatttt actctcaaaa ctagatttta
     8281 cattaagaca attattaaca ccactaatcg cagtatttat aagagaacaa gcagaacttc
     8341 caatactcaa tgcattttta attttagatg tcttttgctg tctttcaata gcttgtttac
     8401 ttttctctag tgcagctaac ttatcttttt gctgtttatt attgtaattt gcctccaaca
     8461 tacctatttc ttttttctgg atttttgtgt aaatttcctt atactcgctt tcacttaacc
     8521 cctcttttgc tctttgaata tcgtcagctt tgtctgcata gttatcatca tctatagact
     8581 taactataat ctccttaatt tggtcaaatt tagttcttct ttcttcatca acacctgcca
     8641 aattataagt cattttatct aaagcatttt ttctatcttc acttagctgt tctccaagta
     8701 caaatctcat accatcagaa tccaatttaa aatctgaaaa attaaccatc ctgttagtcc
     8761 atttgtattc ctcttcttta gatgaaagat tttccttcaa agattttgca ctgtcaaatg
     8821 cccctagaca agcttgacca acaccactaa atgcagtcag taaactcaat gcgctttgta
     8881 ttccagaaag aattaactgt gttttcattt gttcctcaag ttttgcctgt ttctttttta
     8941 gttcctctat gtcttcttgt tcatcttctt tcatctttat aatttcttga atcaacaggt
     9001 tcatttttgc attaacagaa agcattttat tctcaagttc attttttaaa acctctattt
     9061 gttgatttcc agattcaatt tgagttagga tactttcctt atatgtattg aaatatcctt
     9121 cttttaactc atctttcttc ccttgttcca attctcttaa ggtcttatcc agataatctg
     9181 tcaaagaacg aatatctgta acacgatttc ctccaatttc actttcttca atcatctgca
     9241 cataaaatgc tgtcttctgc tcaagtgcaa ataaaatcat actccattcg ctaaaattct
     9301 gattttttcc tacaaaagat tctattctat cagactgcat caatttattt ataaggtatt
     9361 catagatttc ctttgcttct ttataaaatt ctttctttgt attcatatca tccagagaaa
     9421 gtactgcttt atttttcttc tcaaaactat agtacttctg aagtatttct ctatttaatt
     9481 gcatctctat tttctcaact gcatgaaaaa tttcataaat atgtgaaagt tctaaaatat
     9541 cagcttctaa aattcctact gcacacaaca gtctaagatt tctataacct ttaattccag
     9601 aaaaaccaaa tctctcgaaa aattcataga agaatgtatc tattgacaaa gaatactcct
     9661 gctttaacaa ttccttttct tttagtgcat ctccagagtg accagctttt ccctctgctc
     9721 cggctgaacc tgaatcttca cgtttgctaa ttgtccagtc cttatgttct tcataaagag
     9781 tatttaattt ctctccatca tctgctgctt gcttctgatt taaagcatga ttaaacacat
     9841 atctatcaaa ataatagtag tttctatcac tattatccat tggaggagag gtacctatat
     9901 atcttattgt ttcttcatat atacctctaa ttccaccaag acctcctgca ccaccagctc
     9961 ctccagagcc tccttttcct gctaaaccat gatttccatc ttttttactt atagcacttt
    10021 ttaataaagg agcttttcca aggagataaa cctgtccaga cttgcttcca agacctgcat
    10081 ttccaccatt acctcctgtg cctccagttc caccttgttt tcctttttct gcttttacaa
    10141 catagtattt tgtaccttta acattagcaa attcttttga ctctttttta gaacctattt
    10201 tagcatttgc cccagcttct ccagttatcc ctgtatctcc tttttttcca tcttgtccat
    10261 ttccaccacg tccaccatct acgtaaagtt taagttctcc tcttttttct atttctttaa
    10321 aagataatat gatatcacct gaagtacctg cattattgcc atttcttcca ttcattccgt
    10381 cgtaaataga actttctcca accaattttt ctgcatcctt accagatacg ttgattgaat
    10441 aagtcccttc tggtatatgt aatttttctg taagtagtac aatattctta cctgagaaga
    10501 cttctttctc catattacag tcaattttca actcacaatc tccatacata tatatagatg
    10561 acaaaatatt aagttgataa ccagatatta ttttagtcaa atccgataaa ttcactttag
    10621 gtataggaat atatatagaa agtacatttt ctttctcata tgattttata tatgtacaac
    10681 tttgaatctt ctctatccta tactctgcat cttcaagctt tttggcttct tcttccctat
    10741 attcttcttc tgctatccaa tatttgatat cttttaatgt atcttctaaa gtatttttta
    10801 gcaatgattt taacaactca cttagaccaa caatatgagt ctcactacag taactattta
    10861 actctaaaag aagacgtgtt tcttctaata tattcacttt ctgtacgcca tcaccatatg
    10921 aaagttcttg tacaaactcc ataagctctt tcatattatt agtcctacta cataaatctg
    10981 tatacatctt atctaattga atctttttat cttttagact atttatagaa gatagaatgg
    11041 aaatagaatc ttccatacct tttttaacac tttctatata aggaactaaa agtttttcta
    11101 ggtctttata ggttacatca acagcttttt gtatttctat tttacaagaa gcagtaagca
    11161 tcaatcccaa atctgttttt tcaaatgttt caggtagatt tttcatttca ttcaacaact
    11221 tttctggctg tttatctagt ggattacata atttaataga tacaccatct gataatgcta
    11281 catttaataa gtacactaat tccttataac cgtcgtaatc tttctcttga ctatctaggt
    11341 cttcttttgc ttttttcaat atatttctta ttttttcttc tgttacatta ccgattagtg
    11401 ctttaggtat tttattgata agaaagaaga aattttcttt atactttgca taatcaccaa
    11461 gcatatcttt caaatttttg cctgtttcta ttaataatgt atgtctagag tccagtaagg
    11521 attgatagtc taaaataact accatccctt tcacctttga agatgtagaa atagtttccc
    11581 ttacaagcat agcaccagca atcatatact ctgctccacg gttatcaaaa aaacctgggc
    11641 aatcacataa tgaaaaatct ctatttgcaa tatctacaac ttctggacat aaagtcttag
    11701 aatatacacc atttccaatt tcgacagctg aatcttttgc agttatatat atttgtccag
    11761 tctcttcatc cgttttttct tccatcacac agccttttaa ataatttata caagtactct
    11821 ttccaacgcc tgaagcacct aggaataaca aaattgactt ttcttttaag tcatattttc
    11881 taagctcgtc tcttccttct tccaaaaggt ttacgatttc actataaagt ttatttgcca
    11941 tacatccatc cctttctata tttcggaaca atagccttga tatttttctt taataagatt
    12001 ctcttctacc tttgaaacag ctgttgataa cttagtgttt ggagcattaa atcctccatt
    12061 gtctcctcta tctccatatt cagcgtattc tcttgctggt cctgttttac cttcattcca
    12121 acttccacca ctaaattcta tatgatattt aattatacta tccaacttat aatcccaact
    12181 tgtaaaatat cctgtatagt acacagtctt atctatacca cttggagaaa atgaaagtgt
    12241 attgtatccg tgatagccac cacttcctcc atatccacct gaacctggtc taccatttgg
    12301 acctgcttct ccatctttca ctgttatatc acaatgatta attattctat ttttttgttc
    12361 ctctgaatct gaaatgaagt aaatattacc tttcttacca gcatttccac ctcttccagc
    12421 atttccacca gctccacctt tttcaccttt tttgccactt gtaccttttg caaaataccc
    12481 tttaaacatc tgtccgtgga aataacttgt ataccctgca tcagctccat tacttccaga
    12541 agctcctgtt tcaccatttt tcccatctat tcctttagaa ccatttcctc catttgcaat
    12601 cagtgttaaa cgataatttc taatttcttc tttaatatat aagtaaatat tacctgaatc
    12661 tctaccatct tcaccatttt taccatacat agaactatct acaagttctc cgctctttcc
    12721 acttacatta atttttacat tactacctac atcaagattg ttacaagcaa gaataatatt
    12781 tttctctgat gcaaagtcca ttccaaggtt atcatccata taaatagtat ctttagcaaa
    12841 gaacataaat gtagaaacat tgtcatctac ttttctattt ttgaaatatt ccaatacact
    12901 gctaattact aagtttggaa ttaatgctct tatttcaata cctttcttat cattaatatt
    12961 aattgttttt actataaatg gatttaaaat gtatttacca ataaattctc taattatctc
    13021 attaccttct tctccatatg cacttaatct tgttatactg tcttctaact cttcattttg
    13081 tgtaactgta acatttgtca aaattgaatt aaaatcactt acactatata cataatcctt
    13141 tttatttgca acttccatta atttttctct atttggtaat gtaaatacct gtttttgcac
    13201 ttcataagaa actaaaactt tttctaaatt ctttcttatt ataatgaagt tttgttccat
    13261 tatcttctta cttgcctttt ctataataaa ttccatatgc acagaaggta ataaaatttc
    13321 cccagttcca tgttctgtat atttacttaa agcatcaatt atatctaagt ttccttttat
    13381 cttattttgt aatttcatgc aactatcttg taagaataag ccactttgtt tggctaatac
    13441 gttgataaat ctgtctatag taagacttgt tgtactttcc aaccagtttt taaatacttt
    13501 tacaacctca tcatattttg ctatttcttg aatcattgtt atacttccaa taatactgat
    13561 aaaatcttct gtatacgcct taacataatc ttctaggcta gatacgatat tgttttgaat
    13621 ttcttttatc aacgtatcta aaactaatct tgcatctgca ctaattggat actggaatgc
    13681 tgatgtctta tctttaactt catctaattc tagtacttgc tttaataaac cgtccctctc
    13741 atctgatgca agtggcttac aaagatgaat attttttcca ttatctttta gcaattcttc
    13801 aaataaccaa gctttttcct ctatattact gtctaaattt tcgcataatt gttcatattt
    13861 tctttttata acattatata tttgtttttc tgttcttcct aagaaacttt ttgaaataaa
    13921 aaaagttata ctacccttaa aattttcttt atgaagacac atatttaact gttggaacag
    13981 gtctagtact tttgtaagtc tagaatctaa tatatctgct tctggtatca atacaattac
    14041 tgcacatata gatgcagaat ttttaaatac ttcacctaat gaaactgctg cgctaatatc
    14101 atgggctact cccctattgt cgctaaaacc tgcagtatca cataattttg catccattcc
    14161 ttctattggt tttctcatat ttacaatgtt cgggtataaa gtttgagaaa cagctcctga
    14221 accaatctct gccacagggt ctaagcaatc aatatatgat ataccagtat cttcatcttc
    14281 gacttccatc attacacatc ctgcaagata gttaattgca gtacttttgc cacatcctgt
    14341 cccaccaaga attagaatta aattttgatt ttcagcctta tttattgcta cttttccttc
    14401 atttaataag agtgttattt cttctaaaag ttgattgtta ttttctggca tttattttcc
    14461 cccttggtta tatattttat ttaaataaag tttaatccct taagtagatt acaaagtttt
    14521 tagactatat ttaaatttgt tttttatttt ttatttttaa taaatttgca ttttcactaa
    14581 atttgtattt tcactcaata attattcttc tttgtttatt aaaataactg tgttgtataa
    14641 tataatcttt accaacacag ttattctatt taatgcgctt attttattgt attttatgta
    14701 atacagttgt tttttatatt atatgtccat tattttccta tggttattac atatagattt
    14761 ttaattatat ttgtaccatt aatttaaaca ttcctatact actgcttaag ttttacacct
    14821 ataccagctt gaatatttta tttttatttg cttaaatagt tcaactatac tggtttaaat
    14881 aggttatctt tatttgctta aatagttcaa ctatactggt ttaaataggt tatctttatt
    14941 tgcttaaatt agttcaacta tactggttta aataggttat ctttatttgc ttaaattagt
    15001 tcaactatac tggtttaaat aggtttcctt tagtttaaat aattcaccta tacctacttg
    15061 aatagttcac ttttatttgc ttaaattcta taccaatact ggcttaaaca ttatactacc
    15121 atgattgtat tgtctatctt caactacaat aaaaacaggt tctaaatctt tagtagatgt
    15181 tatttttaat tctaaaaaaa cattatcagt tggtaatatt tttacatcat aattatttaa
    15241 attattatta gtgatactat taaatacatc accttgacgt tcaaaaaaaa cattatcaac
    15301 tattacaaaa taaacatctt cttttctact attttcaacc tctatagtat tttgtttagt
    15361 tacatttagt ctcatcttcc acctcctatt aatattactt aattatatta atacttgtaa
    15421 ttgttgcatt atgttcatca caaggaagag gtttaaaaac aactatctct gctaaataat
    15481 tctgtggagg atttgtaact gaagtagcat tgcaaataaa tttaaaattt gttcctgcta
    15541 caacttgagt agaaacaata agtggttcat aaccaacccc ttctaacatt cctacagctt
    15601 cattaaatac ttttaaatct tcttcttcga cagctctgaa ttttgaccat ccaccaacaa
    15661 aaagactcat ataaaaacct cctttatata tatatttatt tatatatagt agtaccaatt
    15721 ttctcaatat tcaatatttt ctgtgtaact ggtcaatgaa aatgcataac tggataaaaa
    15781 tacttataaa ataacttaac tttacatata aatagcttac tttacatgta ataagattat
    15841 tctacaatat gaaatacatc ttgttttgaa tatattctaa atacaataat ggatattttc
    15901 aattattatc cttagaaaat atccattact taacttgttt tatttaaaat cacatagaga
    15961 ctttattatt ttttttattg catgaatagc tatttcaggt atcataaggt gatatacccc
    16021 ctccttgaaa ccacaaaaaa catgacaaac tttacgaatc ttggcttaaa aaactacagg
    16081 tacattcctt atttgtggat tggagtagcc ataacaagca aattttacaa tttttatgat
    16141 gacaaaatca ataaaatcgc ttagtatata acacgaaaca ctacttgttg ctattaagaa
    16201 tagttagata ggaagtatgc attataccaa taaaggaatc aataaagaaa gttaaaacat
    16261 ccctacaaat caaaaatctg ttacattttc tatatttaaa cgttatttac ttacaattgg
    16321 agaaataaca ggttttcctt ctcggtcaag taaaggcgta aatccggctg catttccata
    16381 atagtggaat acatagttca cacctgtttc tttatccacc caaatctccg ttgcatttat
    16441 ttttccttgt gaatatactt tttcaaatct atcttttccc attttataat cccccatatt
    16501 tttgattggt ctataccttc ttcacttaag tcgaaaaaat acataactta tttttttaat
    16561 tctaatatga acacgtaaaa atgtcaaaca gcttatttaa tttcataact tcatgttact
    16621 gttcgtaacc ttgtttgttt acttacacta cattacagca ccattttacc ctttacatga
    16681 ttgtattgca cacagcaaaa atgcttactt tattggattt ttcaactaaa aaaaggtttt
    16741 tttctccaca gaacatttac tattttttat aatatatcta attttactct catatcaaca
    16801 tttaatgaag atataacctt tttcaaatat tgtaaagact ccttaaaaat attagaaaaa
    16861 gattctgcaa atctctcaac atctcctttt tttaaatacc taagtatctc aaaacataca
    16921 tcaaaacgtg ttttcaaaat ttcattatat tcttcatgaa aaaatctctc taaatagatt
    16981 ccaaatatca tatattgttt taaatgcaaa aatgtttcct gtaatacata aatttgtgta
    17041 taatctacta tcttacaaag taaaacaaaa tctggataaa ccttacactc ttcacaatta
    17101 ttttgtaagt accttaaatg agtttctatt ttatctacag ctatcaaatc cattttattt
    17161 cctagtaaaa gtactacatc ctcaagagta atcgtaacaa actccatagc atccaaaagc
    17221 aaactcaacc ttttagctcc ttcttctgta tttatataat catcatcata ttcagaaaaa
    17281 agtgttacat atgttccctt gccattaatt gttttagcaa ttcccaattc atttagctca
    17341 ttataggcac ttctaactgt tggaagtgat acattatatc tattaattat cgaagtaatt
    17401 gatggtagac aatctcctat tttaatattg ccttcaaaaa tttgacaaat tacatctgaa
    17461 acaattaagt catataaata acatttttcc aacgttacag aattaaaact acttgtatct
    17521 atactttgtg gagcacgatt ttctactatt ttaagtatgg attctctaca agctttatac
    17581 atatatgtta atagtttttt aaattcaatt agattcccgt ccaatccata taagtaagtt
    17641 tttcttacct gctctatgta tttattacga aaaaagtcta tacaacaatt atccaagtta
    17701 tcgaaattat aggccaattg tgaaataatg acatcatgct gaataatctc acaaaaatgg
    17761 tgaatcagga aattatttgt tttacttatt agtttattta tatattttag cctttgttct
    17821 ttaaagacta aataattaga actatttttt tccatacaat cgataatctc gtttaattct
    17881 tcaaagtctt tagatgtaaa tcttttaata ttgtacatag caatagaagg aaatattaaa
    17941 ctaaaaatct tatatatttc tgatatttgt aaaatcttct catataataa gtcagattct
    18001 attgattgtt tactcgtact ctggattaaa actggtctag aaccagcttt tgtctctata
    18061 aagccctttt ctgagagcat ttttattaca gtttttatag tattttttga taccatatat
    18121 tcttctgaca tgctttttat agaatcaagc ctatctccaa tagtaatttt accagtataa
    18181 atttgttgaa ttatatgctc aaaaataatt aaatattgtg gttttctctt catatttttc
    18241 ctcttcgata aattagattt attgtttttt gtaaggtata tttactttta tttaataggg
    18301 caaaatagtt gcttattttt tcctcataat ctataattaa tataaaccat ttatatatat
    18361 ggtctaagtt ttataatact gtaatgcagt aaaatagcat attgtagcta tactacagca
    18421 aattaacata ccattgtagt atattaattg gaataatttt aagtacttca aaattccagc
    18481 aaaacatata tgtgtttttg atattatgta tattcgttaa tagaatagct atacttgttt
    18541 tacataataa attatatgat acttgctata tgtaattact atatttaata cacataacta
    18601 tgatacttgc tatatgtaat tactatattt aatacacata attattatac ttactatata
    18661 taattactat acttggacat atagttatca tacatactat atgtaattac tatacttata
    18721 cacataacta ttatatttgc tatatgtaat tgtactatac ttggacatat agttatcata
    18781 catactatat gtaattacta tacttataca catagttatc atacatgcta tatgtaatta
    18841 ctatacttat acacataact actatattta ctatatgtaa ttatactata cgtggacata
    18901 tagttatcat acatactata tgtaattact atacttggac acataattat tatatttgct
    18961 atgtgtaatt atactatact tggacatata gttatcatac atactatatg taattactat
    19021 acttatacac ataactatta tatttgctat atataattac tatacttgga cacatagtta
    19081 tcatacatgc tatatgtaat tactatactt atacacataa ctattatatt tgctatatat
    19141 aattactata cttgacacat agttatcata catgctatat gtaattacta tacttataca
    19201 cataactatt atacttacca tacataatta ctatatttgg acacatagtt atcatacata
    19261 ctgtatatta agatataata ctttctaaat tttatataac acagacacat gcaataaaaa
    19321 agaaggtatc cataataagc tctttgcatg tacctaaata aaaactgtaa aatagaatat
    19381 ataaaatcta atctatttta agtaaataca atttcacttt aaaagaggat gtactatgaa
    19441 ataccatata tttttaatca taactacttt attaataatc gaactatgta acatttacat
    19501 atgcaaaata gcatcttttg gaaacaaaaa tataactata cataagaatt acaaatatat
    19561 ttttttaata aaaccattaa ccttcttgat aataatattc acaaaattac cattaccact
    19621 atattatata ttgttttaca tagtaagtca taaattgctc ttaagcagaa aaagttctac
    19681 taacaaccat tttattccca atatggcagt aataagtttt tgtattttac accttattac
    19741 tgtaagtaca atatcacttg ttaaaaatac aagtccagat ataatatttg caaatatgac
    19801 ctataagtta attagtattt taatagcttc tattttagat tttatcttat ggtactctat
    19861 gaataaaata aaagagcata tttatataat aactcaaaat caaatagagt tcagtctatt
    19921 tttaaaattt atctggttca ctttaggata tatattattt gatagtatac aaattacatt
    19981 tgctccacca agttctctaa cttcctactt tatattggga agtaacatat tattaatctt
    20041 acaactaatt atattcatca atcatatata ttccattgaa aaaaatattt atttagaaaa
    20101 agaacatcat agactaaaaa tgataaaaca agaacaatta ctaaaagtaa atttattaaa
    20161 gaaaaaagct aacattgatg aattaacatc agcatataat cgaaaatttg cttttgaaaa
    20221 aatacagcat tacattgata acaatttatt attttcattg atatatatcg atttggataa
    20281 attaaagacg ataaatgata catatggaca tttagctggg gataactata ttattaactt
    20341 ctcaagtata gttattgaaa acttaggaat agatgacata tttgctagaa taggaggaga
    20401 tgagtttata gttattatgc ctaaatctaa tgaaaacacc acaaaagaat ttatagagaa
    20461 aataagtaaa attatggata ataagatatc ttttagctat ggtattgtag aagtttctag
    20521 cgaaaacaaa atgactgtag aagaaattat taatattgct gatattagaa tgtacactaa
    20581 taaacaaaat aatcataaaa ggaacaataa ctaaaatgaa tatttacatg agcttacttg
    20641 tagtctcact atgtttttta tcttattatc tctatcatta taattttttt aaatatctag
    20701 acataatgat taataactct aaaaataaat acaaagtata tattgtatac tctcttttta
    20761 attatttaat ttttattatt tgtacttatt taaggttaca cctagttata aattggacaa
    20821 tttttatgtt aattttattt atgcaaatta aagtaactta caaactttcc acaataaaaa
    20881 acctttttta ttcgttaaac tgctccatca ttggtttagc aataaatata ttttttaggt
    20941 ctttaatgtc aatagtgtta aatatgccag catatttatt tgatagtaat tctattacaa
    21001 atatttacag agtattttct atattgctta gttttatagt tacaggatta tatttcagtt
    21061 ataaaaatca taaaaataat aagattaact ttgaggcagt tttagaggac aaaaaaaact
    21121 taattttctt attgagcctt atgtttggaa tgtacttgta tctatgtttg agtttgtcaa
    21181 tatactatac tgttggaaat gtattgattt taaaattata tggaatcaag tctgccataa
    21241 tagttttggt aggtaactat attgcagaaa gatatagctt tagaattact caaataaaag
    21301 gttatgatat taaaaatcaa atagaatatg tagaaagtgt aaagacagag tatgaaatta
    21361 ataaattgaa tgatattgta tatacagatt tactgacagg cttctacaat agaccttttg
    21421 taaatcaaaa actatcagag tggacatcaa atcactacaa atttacctta tgttttactg
    21481 atttaaatag cttaaaacct gtaaatgata aatttggaca ccaatttggt gataaataca
    21541 tatccactac tgctcaaata ataaaaaaac ttataaatgt agaaaattcc ttactattta
    21601 gatatggtgg agatgaattt ttattccttt tacagaatac aagttgtgta gaagcagaaa
    21661 aaataatgat taatataaat tatgaattat caaaattaag taatactgac gagtatcctt
    21721 atgctctttc cataagctat ggattagttg agtgggatgg aatatccgat attgaaactt
    21781 taatatcaaa ggcagattct ttgatgtatg aaaataagct taaaaataaa tctactttaa
    21841 actagctagt taagatattc tacaaaatat atgttataag agagtactaa gaatgtcaaa
    21901 atatatagat tttgcaaata ttgaatacag tttactaatg aatgcactgg atgtaagtgt
    21961 tagtaaacac ctattagatg aacactttac tgttgtttgg gctaatgacc gttattatga
    22021 aatgttcggc tatacctagc taaaaagtta aaaatgacca ctgtagccga gggaatagag
    22081 accagttttc aaaaagattt cttgcaagaa atagactgtg atatgttaca aggatacgtg
    22141 ttctctagac ctttaccaat aaaagacttt gaaaaactta tgtttcaaaa ctcctccaat
    22201 taacaatata aaattgctta ttataagtga tattattgta tcaacaaata aaggaattgt
    22261 ctcaaaattg attttttcta atcaaatttt tattttgaga cattctcatt tttttgcata
    22321 agaaaatacc caccaagcta cggtttttct taccgctgtt cggtgggtat tagtagtatt
    22381 atgaaatctg aaaactttaa gtattactac ttccgctaac gcaatcacaa aggtcgaaag
    22441 cttcaaagcg aaaataggct ttttactcat caaacgtcac cgtacctgcc aacccgtcca
    22501 ccgttatcat ttgtccgctc ttaatagttc gtgtggcaat gtgtgtagcc ataacggctg
    22561 ggatgccata ttcacgtgcg acaatgcttg aatggctaag aggaccacca atgtccgtta
    22621 caatcgcact tgcagatgaa aataatggtg tccatgctgg agttgtggta actgcaacca
    22681 aaacacttcc aggttgaaag ctttcaaaat ctgctggact attcagtaca caagctggag
    22741 ccgtcacaac accagtactg gtaccaatgc ccgtcaacac aattttccca tctttctgct
    22801 tctgtggttc ttgtgatata gtttttttgt ttttctctgg caacttggat ggtgacacat
    22861 aacccatgta ttttttaagt tcagcttttc gaacaggtat tatcccgttc atatcagata
    22921 gagacttgtt tttatccaac tgctctataa gtacctccaa ttccgatttt gtgagccaat
    22981 aaatatcatc taaatgtgaa atagctcctc ctcgtatgaa tcgttctgag attttctgaa
    23041 acatacaacg aatcagagga tgtcccattc ccatcaaata aatagcattt tcacgcatag
    23101 gagaggtttc ctgtgcccaa tggagcaact taaaaaatag ttttttacgc aatccaccta
    23161 tgtgttgtaa aatctcttct tctgcatgtt ttctacgctt ttcaaacgtg gtctggcgta
    23221 aaaaaggact ttcccctttt ccttccacaa atgtttttac ggattcaaaa gttggtgtaa
    23281 gtgtttcctg cggtgtggaa tgcgcaaaat caaactcata agcggtacga ccaaactctt
    23341 taaagtactt attgattcga tttttccatt ctatccatac ttcctgtgaa actccaacag
    23401 gcacaactga agacatgaaa tcttctgcta tctttattgc agaactgttt tgcagataaa
    23461 ggttgatgct gttattttgc tttacccatt ctgaaatacc ccacaacgcc ttttccgatt
    23521 gcagaacaat cgtatcaaac ccaagcagga aaacggaagt atccgttata ccagcacgtc
    23581 gaactgctcc ttgaaataat tttgtaaata atgtttcact gatagaagca gcaggcaatg
    23641 taagctgaat ccttgtgaaa taaatacatg cggcataaaa tattgtctga ataccctcca
    23701 taatttgatg ggcgttcata gtatgcaggg gcttttcttc ccattgttta atagcatcct
    23761 catatccttt tcgtgccgtt tcccagcgtg caacgctgtt cataagtgtc ctacgtaaag
    23821 agcgtgggga aagggatttg atagcaatca aaagtggctt tgatttagca gaaagataaa
    23881 catatccatt aataaccgta tatgccccgt tttttggtaa taattttttc gcacttttac
    23941 caaacatatc tatccataaa agctctgatg cacggtttac gatttcaaga ccaagcgtgg
    24001 caaacaaagg tgtaacagga tttggcaagt gttctgccaa acttcccttt gtgtatacaa
    24061 catcctgttc tggcaatacc cattctggtg gcagaacagt aattggacga gcctgaacaa
    24121 tgtacaattt atctttttcc agtgcccact ctacatccat aggcatttga taatacttct
    24181 caattttctt tccaagctgt atcagttgca ttacctgatt acgagtaaga gcatattttt
    24241 ttctcaacct ttcaggaacc atggtttctt ctgttccttc tgaagtacga accgtcataa
    24301 tctctttatt tgccacttca tacgatataa ttcgttcgga atccttatcc actacgattg
    24361 tatcgggtgt aactaagcta gaaaccaccg cttcaccaag cccccatgca gcgttaataa
    24421 tcatttcgct acgtctacca ttgattgggt ttaacgtaaa cataatacca gatgaatcgg
    24481 aaaatgcaag cttttgtact acaacagcaa gtgcgacaat ctcgtgcttt atattattct
    24541 tcacacgata agcaatagca cgagctgtcc ataaggaagc ccagcatctc tttacagcgt
    24601 caataacttt atcttcacct tgtatgttaa gataagtttc ctgctgacct gcaaaagaag
    24661 cgtcaggtaa atcttcagca gtcgcagacg aacggacagc caccgatatg ttccctaatc
    24721 cggcataagc cgttttaatt gcatccgaca cttcctgtgg catttctcca ttatggaaaa
    24781 gctcccctat tttcttagat acatcttcaa gttggctggt attattggaa tcaataccat
    24841 ctagcagttt attaattcga gactgaatat ggttcgtctc aacaaaaatc tggtatgaag
    24901 cagtcgtaac atgaaaaccg tctggcacag ggatacctgc tgtcaatagt tttgataaag
    24961 acatcccttt tccaccaaca atttcaatgg ttgcttgttt atgttccagt ggcaagacat
    25021 aataaatcat aatcattcac ctccatcatt cataattccg tgcagaaata tattcattgt
    25081 ttcatccaac attttttccg aactatccga tggtcgtgac gtgtagctga ttgataacat
    25141 ggagttaatt aacaaacgtg tcacaagagc ggtatccgtt ttgcgaaaga tgcctgtggt
    25201 cacaccatta tcaattaccg attttataat atcttgatat gcataacgag catctttaag
    25261 tctctttcga tactcttctt ctaaataatc agattctgta tttaaccaca tcagtacagt
    25321 tctatactgc tctggaaatc taagatgatt aagcatgatt tttctaaggc actgttgtgg
    25381 tgaaggttca ccatcaataa tcctatgaac ttgttggata gttttttcta tttctttctc
    25441 aactgcatat acaacaatct cgtctttcgt tttgaaaaaa tcgtatagac ttgactttcc
    25501 catgttggct aaaactgcaa tttctctcat tgatgttttt tggaatccat tttcagccat
    25561 gagactcaaa gcaacagcaa ctattttttc tcggcgttct gcttgttgtt cgggtgtaag
    25621 cacaattcct tttggcatag ggcgatacct ccttttagcg accactggtc gtttcttttt
    25681 tatttatcat acagcgacca acggtcactt gtcaaggtta atttttttca ttgtatatgg
    25741 tactcgagat aaaaatgttc attttagaat ttgctctatt taagtagaaa taggcttcat
    25801 cattagaaaa caagactata aatttgagat atttatcatg ttcaaatttc atcaatatca
    25861 aagtttaaga tattaataaa aaaagaatta ctaagaaata acatataaag attcaaaaaa
    25921 ggagttgaac ttaaaattta attacaaaaa atcaaattta agacaactcc tttattcttt
    25981 atctctacta ttctttatct ctatttattt attacaactc cctaacattt accactactg
    26041 ccttaggtct agtcatagcc tcgattgaat acttaacacc ttgagttccc ataccagatg
    26101 cctttacacc taaaaatggg aagtggtctg gacctctttc agtcttatta tttatttgaa
    26161 cagtaccaac ttcaagttta tcagctatat aaaatgcctt atcaatatca cttgtaaata
    26221 ctgatgattg aagcccatac tctgattgat tagcaatttc aattgcttga tttatatctt
    26281 taactcttat aattggaagt actggaccaa atggttcttc ccaagcaatt ttcatatcaa
    26341 catttacatt atctaacaat gttggataga ataaattatt ttttctaaca ttgcctgtta
    26401 ttaactttgc tcctttatgt aatgcatcat caaccaaccc ttgaacaaaa tcagttgctt
    26461 tattatctat aagaggagtg ataactactt catccattgg atttccaatt gtcaacttct
    26521 ctaccaatgg ctttattttt ccgaccaatt tatccgccac actctcttgt actaaaattc
    26581 tctttactgc cgtacatctt tgtcctgaat atgaatacgc tccagatact atattcttag
    26641 ctgcaaaatc taagtctgca tcttctaata ctatagccgc atcttttcca ccaagctcca
    26701 ataataatgg taccattcca gatattttag atatatgctg accaacttca gtactaccag
    26761 taaagttaat aaaattaatt cctttatgcg ttacaacata atctcctatc tcactacccc
    26821 tacctgtaat agtattaagt actccatctg gtatcccagc ttttctcata atttctacca
    26881 catgaagtgc gctaatagcc ccttgtgtag caggttttaa tacaactgca tttccaccaa
    26941 taagtgctgg agcaatcttt gacattgata aatttacagg ataattaaaa ggagatatag
    27001 ctaaaacagt acctaatgga acccttgaaa cataagacat cttatttctt gttccaccag
    27061 ggaaattatc tccactaatg gcttcaccct ccatattttt tcccacatca gctgtgtatc
    27121 ttaaaaaatc tgctgttctc tcaacttctg cccttgcaga ttttatgtct ttggctattt
    27181 caagtaccag tacattagct atctcatcta tattttctaa caataaatca gcagccttat
    27241 gaaatatatt ggctctcttg tatacaggca tttcagccca tacttttata ctctcttttg
    27301 tgttttttat aacctcatct acctcatgtt ttgtcataga ttgtactttc ccaacaagag
    27361 aattatctat tggtgagtaa atttctattg gctttctcga attagattct acccattttc
    27421 catttattaa atttttatat acattttctt ttgttttaag ttcattaaac atatttttat
    27481 tcctctctct gatataactt tgtgtattta aattatagcc atttattcgt tatgtaaaca
    27541 tttctttttt tcataaacgt ttacctataa aaaaaatttt aagttaacgt ttttctacat
    27601 tcttctattt taataacata ctcgctccaa atctatatag gtcacacttt aatatcaata
    27661 tactaatata ccaatatacc aatataccaa tataccaata taccaatata aatattataa
    27721 acataaatca cttgacaaat ttataagtag acaatattat atatctatga ctcaaaatat
    27781 ttttaaagtc atatatatca tatatagata gaaaggattg ataaacatgg ctaaaagttt
    27841 tacagaaata gaaaaagaca atataagaaa aagactaatt tcagaatgtg aactcaactg
    27901 gtctaaatat ggatacaaaa aaactaatat tgatgaactt tgttcaaaat caggaattgc
    27961 aaagggttct ttctatacat tttttgattc aaaagaaaag ttattttttg aagtattaaa
    28021 tggaatccaa aacagactta tatcatcagt agatgaaatt ttaagtaaca tgcctccaaa
    28081 agaaggcttt gcaaaatcct taaaaatgtt atatcgatta tatgataaaa atccattttt
    28141 atattcacca aataatcctg atttcatcag cctaataaat aaactacctc aagatatgtt
    28201 tttacaactt gagtccaata acatacaaag tttttacgac ttaattgaga gacacaatct
    28261 caagttgaag atagaaaaag aaaaagtttt tggtgtttgt aatgctctac tatcaagtct
    28321 attggtaaaa gaaaatattg aatataacta tattgaagtg tttgacttta tggtggataa
    28381 tcttgtagaa cacatacttg aataacaatt taaaataatt tttctaagta tttttttata
    28441 ctaaatagga ttaatatgat tgaattaata caaaaaataa acaatataat ttataaaata
    28501 aatgataaac taaatatcac acaaaactta tagaccctag agcaaaccca ttccactcta
    28561 ctctaagcca tctataatag tttgtatatt tctaataata ttagatatta aagtaagaaa
    28621 aaaattcctt ataataattg ctattatatc aattattata aatattttaa taatcttaga
    28681 taggtaggta catatatggt aaaatcctcg aacactttta ataaaaactt caacttgatg
    28741 atattaggtc aaataatctc attgtttggt gcatcaatac ttaaatttgc tttatcttta
    28801 tacatacttg atataactgg taaagcagaa atatttgcta ctatactagc agtatcttca
    28861 atcccaatta tagtattctc ccctattggt ggagctattg cggatatatt caacagaaga
    28921 aacttaatgg ttatttttga tttttcaagt agtcttacag tactaatctt agcagtattc
    28981 ttattcaaca ataatggttc aatattactt gtaggaatta ttatgaccat attatcagtc
    29041 ataagcacaa tgtatcaacc aactgttcag tcaagtacac cccttttagt tgataatgag
    29101 catttaatga atgcaaatgg tattgtagct ggagttgcat ctcttacaaa tattgcagga
    29161 cctgtattag gaggtgtctt gtatggtgtg attggaatta atgcaattat agttatcagt
    29221 tgtgtaagtt tctttctatc tgcaattatg gagatattta tacagattcc ttttatcaaa
    29281 caagagcaaa acagcaacat tataaaaact atatttactg atataaagga tggtattgtt
    29341 catatatgta aagaaaatag atttatacta aaaatacttt ttttagctat agcaataaac
    29401 ttatttttaa gctctatgtt aatagttggt atcccctata tagtaaaaat cactatggga
    29461 gctagtaact tcttatatgg tctagcagaa ggagcaattt cttttagttc actggtagct
    29521 gctgttagta ttggtatttt ctcaaaacat ctaaaaatca ctaatttgca tatttccttt
    29581 attatatgtg cagttatgtt cataccaatg gcggtttcag tatatccatt tatattaaac
    29641 acaggatttc cagttccatt tttaatcttt atagtatcat ctatgctaat attttttaca
    29701 acgactctta tctcaatata tgttataaca gtcacacaat taaaaactcc aaatgaactc
    29761 ttaggaaaag taatgtcaat tctttttgca ggaacagcaa ttgcaatgcc ccttggtcag
    29821 gttatgtatg gtaaactttt tgatttatta tcagatagag tgtacatcat tatactagta
    29881 gttggattta ttacattact tatagcattt gtatcaaaga taacattgag gaacgaaaat
    29941 ctttagatac aaagaattta gatataaaga tttaattttc actaatttct gaatttaaat
    30001 gcaaaattgt gtatcagtat gttgacgttt tagaaattga ttgatataat caatggaaaa
    30061 taaactttat aaaaattcaa aatattcagc tagaggagtg ataaaaatga gttacatgcc
    30121 aactttagaa caagcatggg aaatattaag aaaatacaat aaggaagaat ttcatataag
    30181 acatgctcaa attgtatctg gagttatgag atactatgca aaggagtacg accctgaaag
    30241 agtagaattt tgggaaatag ttggactttt acatgattta gattttgaaa tgtatcctga
    30301 tgaacattgc gttaaacaaa gagaattaat gacagagctt gatttagata aatcaataat
    30361 cgattctaca ataagtcatg gatatggatt aactggttca gatgttaaac cagagcaatt
    30421 tatggagaaa gtattatttg ctgtggatga acttactggt ttaattggtg ctgctgcaat
    30481 aatgcgtcct tcaaagagcg tttctgattt agaattaaaa tctgttaaga agaaatttaa
    30541 agataaatca tttgctgctg gctgttctcg agacgttata agaagtggtg cagaaatgct
    30601 tggatgggaa cttgataagc ttatccaaac aactatagaa gctatgagaa gcttgattcc
    30661 agagatggca atatagctcc tccatacata taccatagaa gttacatctt taaagagcat
    30721 ttctcttttt gatgcaactt ctttttatat tttgtagaat aaaaagttta atattttaaa
    30781 ctcactatat aataaataat ataatgtata atagtcataa actttctaac cttaaaagtt
    30841 tctaataatc aataaacaat tctaatatac aaatgtcatt atatgattta attatatatc
    30901 ttcaatttat atattaaaat aaatatatat atattaaatt cttatacacc aaatttttac
    30961 attaaacttc tatgcaccac atttatacat attaaattct tatatattaa atttctatgc
    31021 atcactttta tacatactaa attcctatat attaaacttt tatgcatcac ttttatacat
    31081 attaaatcct atatattaaa cttttatgca tcacttttat acatattaaa ttcttatata
    31141 ttaaacttct atacatcact tttatacatt aattcttata tattaaactt ctatacatca
    31201 cttttataca ttaattctta tatattaaac ttctatgcat cacttttata cattaattct
    31261 tatatattaa atttctacac atcaaattta tatacattta aatcacatga ttaactgctt
    31321 tcttcacatt tctaagtgga atagcaattt taactacaaa ttttttatca tcatattcaa
    31381 ttacaacatt accaccatac ttatttacag agttttttac actttttatt ccaagtccat
    31441 gaagagaacc ttttttgtct gtcaaaatat ctccattttt agtaataatc ttattcatct
    31501 tagtattttc aactcttatt aaataaaaat tttgcataac atcaccttta acacaaataa
    31561 ctctatttcc actatcaatt ttattacatg cctcaattgc attgtctaaa atatttgaaa
    31621 atatatgaca tatatccact aattcaacag cttcacaaca actaaaattt attcctattt
    31681 ttagttctat atcattctta tcacaaacct ctttcttttc acttaatata gcatctaata
    31741 taaaattacc agtattaaat aacaagtcat tactctgaat ttctacctgc aaattattta
    31801 aataagaagt aacatcaata ccttttttaa tcatagcatt tatacacaat atgtgatttt
    31861 tcatatcatg acttagtaat ctagttttta aatatttctt ttctatatct tcataatact
    31921 cattttcata cttcaaagta tttttaatca tattgttttc tacaactaaa gtattatatt
    31981 gctgtaattt tttagcaatt aaaactaaag tgatacttga tataagcaat acaacagaaa
    32041 tcataacgaa ttggaaatta tgtattaagc taccagaccc tggattaaat atgtattcaa
    32101 aaattacaaa aaaagatgct atattagcga ctataggaat aactatataa aataatgtcc
    32161 ttttatttat ttctatatta agttttatct ttaaggctct atataaaagc aaagacacaa
    32221 tcaaaagtat ctttcccaat attatagact caattctgta tatatctccc gtccccatac
    32281 tactcatgtc atttatagaa ttaagccaaa ctgttaaaga tatactcaaa gcatctgtgg
    32341 cgattagtaa catccaataa attaaggata ttaatacaga ctttataaac cccacatcat
    32401 atgagatttt ataaaatgca atagtaatta tcatagatat aattactctt atatttggag
    32461 gtacatcaaa aaaatatatt atgtcagcaa aaacaataat acctaccata caaaagttta
    32521 tttttttgtt agaaatcctt ttctcacttg tataatctag cattttctta caaagaaacc
    32581 attctatcat aaaggatatc gcaatgaaaa tattccaaaa taagcttgac tttatcataa
    32641 tactttctcc taacgtatta ctaaattatt tttttatatt ttatgtataa tttaattata
    32701 tacgactcta cacgtgtagt aaatagattg tcaaaattat tccaaataag tccatatttc
    32761 ttttcttttt taattatatc actaaatttg ctaaattgaa ttaaactttt tattgctttt
    32821 tttaataata actatataaa tttatctata actaggtata aaatatatta tttataaagt
    32881 ttt
//
LOCUS       DAGSRM010000037        29706 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.037, whole genome shotgun sequence.
ACCESSION   DAGSRM010000037 DAGSRM010000000
VERSION     DAGSRM010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29706
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.037"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            335..913
                     /locus_tag="MJE33_001903"
     CDS             335..913
                     /locus_tag="MJE33_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBY9647839.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            952..1530
                     /locus_tag="MJE33_001904"
     CDS             952..1530
                     /locus_tag="MJE33_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBY9647840.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLKHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            1616..2245
                     /locus_tag="MJE33_001905"
     CDS             1616..2245
                     /locus_tag="MJE33_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBY9647841.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            2235..4994
                     /locus_tag="MJE33_001906"
     CDS             2235..4994
                     /locus_tag="MJE33_001906"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBY9647842.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     LKKTA"
     gene            5081..6643
                     /locus_tag="MJE33_001907"
     CDS             5081..6643
                     /locus_tag="MJE33_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HBY9647843.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGAIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDVHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            6644..7780
                     /locus_tag="MJE33_001908"
     CDS             6644..7780
                     /locus_tag="MJE33_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBY9647844.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            7844..8920
                     /locus_tag="MJE33_001909"
     CDS             7844..8920
                     /locus_tag="MJE33_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HBY9647845.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNSHFITGILIPKANSELIENCIEALDFMNLQDMYVI
                     PIVETKEFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            8898..10136
                     /locus_tag="MJE33_001910"
     CDS             8898..10136
                     /locus_tag="MJE33_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HBY9647846.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGIRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELDCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITMASKESLKNEKEKILFLGTEEFMYIPMLFAKEKENQYDIYYHST
                     TRSPIINIDKNNYPIKSKFMLKSFYNIDIQNYIYNIDKHNFSKCYLFVELNKSENSYL
                     EFIDIIKKTSIKELTIVCCS"
     gene            10190..11278
                     /locus_tag="MJE33_001911"
     CDS             10190..11278
                     /locus_tag="MJE33_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HBY9647847.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNISLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKYFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLIGKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
     gene            11306..12097
                     /locus_tag="MJE33_001912"
     CDS             11306..12097
                     /locus_tag="MJE33_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HBY9647848.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYTNIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKCDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDLSRFNLDSIKEYTNI
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFNGMSGTEEILSNILDENCLLNI"
     gene            complement(12212..12766)
                     /locus_tag="MJE33_001913"
     CDS             complement(12212..12766)
                     /locus_tag="MJE33_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBY9647849.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTQKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            12962..14164
                     /locus_tag="MJE33_001914"
     CDS             12962..14164
                     /locus_tag="MJE33_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HBY9647850.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            14221..15201
                     /locus_tag="MJE33_001915"
     CDS             14221..15201
                     /locus_tag="MJE33_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HBY9647851.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLSEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKDILK"
     gene            15444..16397
                     /locus_tag="MJE33_001916"
     CDS             15444..16397
                     /locus_tag="MJE33_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBY9647852.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RIPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAIFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            16387..17343
                     /locus_tag="MJE33_001917"
     CDS             16387..17343
                     /locus_tag="MJE33_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBY9647853.1"
                     /translation="MKLSLNQKIYFVSILLFISMALFIIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKTLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIHFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            17340..18095
                     /locus_tag="MJE33_001918"
     CDS             17340..18095
                     /locus_tag="MJE33_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBY9647854.1"
                     /translation="MIEIKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRGLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVEKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            18187..19152
                     /locus_tag="MJE33_001919"
     CDS             18187..19152
                     /locus_tag="MJE33_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBY9647855.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKKNESKTSENSNNTMKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAETLVGLPKASLPASLEKYKDDKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
     gene            19633..21492
                     /locus_tag="MJE33_001920"
     CDS             19633..21492
                     /locus_tag="MJE33_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBY9647856.1"
                     /translation="MKRFLRRIILVLFILLIFISFISIKLIHNVGDYGKLINYVGIVR
                     GASQRLTKLEMNHQPNDELIEYIDEILQELITGHGDYGLVITDCNEYNEDLLLLEKKW
                     EDLNIEIKKVRMKEQNNQLLSISEEFFSLANDTVFKIENFSKEKSNYLMTLIIIISII
                     GILACIILILQYSKKMVRLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICIHKDK
                     KFAVFYIDFENFKYINDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVALRC
                     YVDKEDLVVRQRDVDNELINITNEIKNKHSITVVSGICCIEDVNEKLSIDGLINRANF
                     AQKTVKNKPGTNYAFYNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHSQKI
                     NCAEALVRWLTPDKGIISPAIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENKPFV
                     QISVNVSRIQFYNTKFVETYSNIQNKYRIPKNTIEIEFTESVAFENQKHLLEIIHDLH
                     ENGFTCSLDDFGKGYSSLSVLKDLPFDVLKLDSMFFKASLDKDKEKIVIKNIVHMLKE
                     LNITTVAEGIECKEQVEFLRDIGCDLVQGFVFYKPMPILEFEKILDKEFVYNS"
     gene            complement(21784..22383)
                     /locus_tag="MJE33_001921"
     CDS             complement(21784..22383)
                     /locus_tag="MJE33_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBY9647857.1"
                     /translation="MAIELKKGQKINLTKKENSDLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGDDRTGSNTQGENLRIN
                     GNKIKDIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYNMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(22486..23283)
                     /locus_tag="MJE33_001922"
     CDS             complement(22486..23283)
                     /locus_tag="MJE33_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBY9647858.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(23396..23502)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(23594..24580)
                     /locus_tag="MJE33_001923"
     CDS             complement(23594..24580)
                     /locus_tag="MJE33_001923"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBY9647859.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVTNISEHLHSKMDILEFKDAIMDYLSKTNTDNTNYCLSEHD
                     IKQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVAFVENKLIGIRHEENALRNTLDSIEINDYFLGADTDILVSGILGVK"
     gene            25064..26794
                     /locus_tag="MJE33_001924"
     CDS             25064..26794
                     /locus_tag="MJE33_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HBY9647860.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLAIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIVISALLLKEFGPMRKAEIKSRSRKISI
                     DLDEGVEELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGDDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVVKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFVGIQIIGKK
                     VDEAEIELVE"
     gene            26904..26984
                     /locus_tag="MJE33_001925"
     tRNA            26904..26984
                     /locus_tag="MJE33_001925"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26938..26940,aa:Leu,seq:tag)
     gene            27319..27834
                     /locus_tag="MJE33_001926"
     CDS             27319..27834
                     /locus_tag="MJE33_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HBY9647861.1"
                     /translation="MEKYIWLFPLLFIFHDMEEIIGFGIWLKKNKSMLDKKYPFISKV
                     YENYSTEGMAFAVFEEFILCIIFCILTVITENQYVYLLWLGSFIAYTLHLVIHIGQSI
                     IIRKYIPSLITSIICLPISIWCISKSIYIVDCEMSTTILYSIIGIIIVALNLKFAQSL
                     IGKFTKWMSNI"
     regulatory      28326..28430
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            28640..>29706
                     /gene="gcvT"
                     /locus_tag="MJE33_001927"
     CDS             28640..>29706
                     /gene="gcvT"
                     /locus_tag="MJE33_001927"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HBY9647862.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHEKVRK
                     SAGIFDVSHMGEVQIKGAESEKFIQNLVTNDISTLKINDIIYTPMCYENGGVVDDLLI
                     YKFGEEDYLLVINAGNIDKDVAWIIKQSEGYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSTKVCGCPCLVSRTGYTGEDGFEIYCKNKYVEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPIEGGLSIFVKTNKESFIGKSILSK
                     EKESGAKRKLVGFEMQGKGMPRNGYDIRIGDKTVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIV"
BASE COUNT        11492 a         3192 c         5092 g         9930 t
ORIGIN      
        1 ggtagtagtg gcgaagacac cgtccgaagt agcttggctt tgtaaccacc tctgttgtac
       61 tgtttaagta taaataagtg tgttagccac caagaatccc cttgctttag ctatggggag
      121 tgtgtcaaat attaaacata gcttttatac atttaataag gtatgtaaaa tctatgtaaa
      181 aagtaagaaa tataaacatt tttttaatta tattttaaaa atatcaagca agttttcttc
      241 acaaatgaat aaaaaatgtt ataatatgat taaagaaaaa gataaataat tagcaagatt
      301 tgtttagcta aaattaacaa gggagcgata aattatgggt ataacattat caaaaggcca
      361 aaaggttgat ttaactaaag gaaatccagg tcttaaaaat attttagtag gtttaggatg
      421 ggatactaac aagtatgatg gtggatttga ctttgattta gatacagcag catttttaac
      481 aggagcaagt ggaagtgtta ctaatgatgg agattttgta ttttacaaca atttaaaaca
      541 tacatctgga gcagttgagc atctaggaga taacagaact ggcgaaggtg atggagatga
      601 tgaacaaata gttgttgatt tatctaagat tcctggagaa ataagcaaga tttcttttac
      661 tgttacaatt catgatgcaa cagaaagaag acaaaatttt ggacaagtaa gtaattctta
      721 tataagaata gtagataaag acacaaatga agagttaata aagtatgaat taggagaaga
      781 ttttagtata gaaacagcta tagtagtagc tgaaatatat aagcacaatg gagaatggaa
      841 gtttaatgca ctaggttctg gctttgaagg tggtcttgct gcattatgtg gaaactttgg
      901 aataaactta taatttcaac tataacaata aactaaaggg ggatattttc catggctata
      961 gtattaaaaa aaggacaaaa aatcgattta actaagggaa atccaggtct taaaaatata
     1021 aaacttggac taggatggga tacaaattca tttgatagtg gctatgatta tgatttagat
     1081 gtaagtgttt tcatggttgg agaatctcaa agagttgaaa aagatgaaga ttttatattc
     1141 tacaataact taaaacatcc atctggagca gttgaacact taggtgataa tagaactggt
     1201 gaaggtgatg gagatgatga ggaaatatta gtgaatttag agacaatgcc aaagcatata
     1261 caaaagatag ctgtaacagt tacaatatat gaggctgctg aaagaagaca aaattttgga
     1321 caagtaagta actcatatat aagagtatta aattctgaaa atgatgaaga aatattaaga
     1381 tatgacttag gtgaagaatt tagtatagaa actgcaatta cagtatgtga gatatataag
     1441 tataatggag aatggaagtt tagtgcagtt ggagcaggat ttgaaggtgg cttagaagca
     1501 ctttgtagaa actatggctt aaatgtttaa tttaagcaat ataatatatt aaatataaat
     1561 aacctataca aataatgtat aggttatatt atataaaatt ttgtggaggt ttagtatgtc
     1621 gattaattta tcaaaaggtg ataaaataga tttaaaaaaa tctaacccag gactgagtaa
     1681 catacttgta ggtctaggat gggaccctgt gcaacaatct ggtggaggtt tttttaaatc
     1741 gttatttggt ggaggacaag ctgatataga ttgtgatgca tctgtattta tgcttaatca
     1801 agaaggtaaa ttaagtggaa taaaagattt aatttatttt ggaaatctaa aaagtgcctg
     1861 taaatcagtt ttgcatacag gagacaatct aactggtgag ggtacaggtg atgatgaaca
     1921 aatcctagtt aatttggata aagttccatc aaatattcat aaattattgt ttgtagtaaa
     1981 tatatacaat tgtgtagata gaaaacaaca ctttggaatg atagaaaatg cctatataag
     2041 agttgaagac caaggtaata agaaagagat agcaaaatac aatctatctg ataattattc
     2101 tgaaaaaact actcttatag taggagctat atatagaaaa gatggaagtt ggcaatttaa
     2161 agcaattggt gaaggaacaa aagatgcggg tcttaaagaa gtaatgcaaa atttagacag
     2221 gattgagtgt gcatatggaa tttaataatg atatcaaata ttataatgta gataaagaag
     2281 aagttacaaa agacttatct gttaatcctg aaaaaggttt atcagaatca gaagtcaaga
     2341 ctagaagaga aaaatatggt ttgaatgaat ttactccaaa agaagagggt agtttttggg
     2401 atgatttaaa ggaaagttta agtgaaccaa tgatagttat cttgattatt gcagcagtag
     2461 taagtgcagt aattggggaa actcatgatg caatagggat tgtaggtgct atagcaatag
     2521 gtatagcaat aggtatgatt acagaaggtc gttctaagaa agcagcagag gctctttcta
     2581 agttgactga aaacatagaa gtaaaagtat tgagagatgg ggaagtacat caaatttcta
     2641 agagtgaatt ggtaccaggt gatatagtat atattgaaac tggtgacatg gtacctgctg
     2701 atggtagact tatagaaagt ataaacctta aaattagaga agatatgtta acaggagaat
     2761 ctgatgacgt atctaaaaag tgtgatgtag tagtttctat ggaagagata gaatctaaag
     2821 gagcagtagt agtacaggag ccaatacctg caaaacagat aaatatggtg tttggtggca
     2881 cacttgtagc ttatggtaga ggagctctag ttgtaacttc aattggagat aatagtgaaa
     2941 tgggtaaaat tgctcaaaac ttatcagaaa cagatgagca aactccactt cagataaaac
     3001 ttggaaattt aggtggtttg atagccaaag tttctagtgc agtagcagga ttactattta
     3061 tatttatggt tttccaaatg ataagtaaga atgtattaaa tgtagatatg tctggaatat
     3121 taccgttttt agaatcaata gacccagtta aaacagcatt tacagtatgt gttgcgttga
     3181 tagttgcagc agtgccagaa ggtcttccaa ctatgataaa tatgacactt gcaataacaa
     3241 tgcaaaaaat ggctaaaata aatgcattgg ttactaaaaa agaagcttgt gaaacgatag
     3301 gttctgtttc tgtaatatgt tcagataaaa ctggtacatt aactgaaaat agaatgactg
     3361 tagaagttgc atatgttgat ggcagatata ttgaaagttc agaagaggaa ataaatagtt
     3421 attttgaaga aaactgtatg ataaactcta cagctgatgt agagcacaat gatggtgata
     3481 ttaagtattt aggaagtgct actgaatgtg cattgctttt atattacaaa aacgtagatt
     3541 atagacaagt tagaaaaaat tctaatatag tagctcaaaa tccttttact tcagattcaa
     3601 agagaatgac ttctgttata ggtcaagata atcatcatgt actattatca aaaggagcac
     3661 cagaagtctt acttgaatta tgttcacata ttcaaagagg aaaagacata gttccattaa
     3721 ctgaaaatat aaaacatgag attttagaag aaataaagaa attgcaaata aaatcaatga
     3781 gaactttagg gtttgcatat aaagaaatct cacaagctga agaagaagca gcagtaactg
     3841 ctgaaagtga tgctatgaat gtaagtgcaa tggaaaacaa tttagtattt agtggttttg
     3901 ttggtataag agacccactt agaaaagatg ttatagagtc tgtaaataca gcaaataaag
     3961 ctggtgtttc tgtaaaaatg cttacaggag ataatataaa tacggcaaga gctataggtg
     4021 aagagttagg cctattaaaa aataatatga gagtggttga agcttcatat atagatactt
     4081 tagatgatga agaattaaaa caagagatac aaagtatttc aatcgtggca agaagtaaac
     4141 cagatagtaa aatgagaata gtttcagctc tgcaaaagag cggagatgtt gttgcagtaa
     4201 caggtgatgg tataaatgat gctccagcac tttcaaaagc agatgttggt atagcaatgg
     4261 gtatatctgg tacagaggta tctaaaaacg cagcagacat aattttaact gatgatagtt
     4321 ttagtaccat tgtaaaaggt attaaatggg gtagaggaat ttatgataac ttccaaagat
     4381 tcgttcaatt ccaattaact gtaaatgtaa ttgcattttt agtggctata atatctcaag
     4441 ttatgggaca agagatgcca tttactacaa tacaattatt atgggtaaac atcattatgg
     4501 atggaccacc agcactagca ttaggtttag agccagttag ggaccatgtg ttaaatagaa
     4561 aacctgttga tagaaaagcc aatataattt ctaagtcaat ggtgtataca atagtattaa
     4621 atgcatttta tataacagca atactaatgt tacaatctac atttaatttc ttaggagttg
     4681 atgaagcgca taagggaaca gtaatgtttg cgttatttgc ttttagtgca ctgtttaatg
     4741 catttaactg tagagagttt aatgcagata gtatcttccc taattttaca cataacaaat
     4801 tagcactaca aataatagtg ttgacaggaa ttgcacaagt aatatttact cagctattcc
     4861 aagatttctt taactctgta gcaatggatt ttatgacatg ggtaaaagtt ttatgtgtag
     4921 cagcaagtgt aattgtggtt aatgaagttg taaaatgtat aataagactg gtaaaactaa
     4981 aaaagactgc ttaaaacaaa ttttttagaa gaaaacagac tgactcaaaa tatgaatttt
     5041 gggtcagttc tgaataaaca tttaaattgg gaggatagtt atgggtgact caaaggtatt
     5101 tgaaaaaata ttctccagcc aatcagatag aggaaattac actccttcaa agggatatct
     5161 aagttatttt atttcgtata ttggtttaga agatgaagtt ttgtacaatc tcgagatatt
     5221 taagacaaaa caaaatattg atagcaaaaa agatattgct ttatttactg atgttatagc
     5281 aaatcctagt gattttgata ttataaatta ttttaaaagt ggacttcaaa aatatagaac
     5341 tagcatggaa gatgtagata taaatatttt gggatttgaa gagattgatt ataaaataaa
     5401 gcaagcaatg gatagagtgt taaaagaaga ggaaaaagaa tttactaatg atagagttaa
     5461 acaaaatttt atagtgaaaa taatggcatg gataaaaatt tatatcggtg ctttagatat
     5521 aaataaaaat gaagctccaa aagtaatatt ttatggagat ataaaaaaac atgaagttta
     5581 tttactctta atattatact tggcaggttt tgatgtttta tatcttaatc caaattcaaa
     5641 gagtaatata gatattttaa aatcagaaag atacaatata gagtttgaag aagccaacat
     5701 aatagaagaa aaaatttctt ttgaagaaag ggttatttta ggggaaaaaa tagataaaag
     5761 ttctgtaaaa aaagctttta ctgttggggc agaagcatca aagcgtataa gtgaggaatt
     5821 gttaaatgat gcaggattta taaaaccttg gcaactccaa gatagaaaaa taaaaaatct
     5881 acttctaagt tcaactgttg atgaaatatc aatatactgg aatcaaccac ttaaattaag
     5941 accaggattt aagtttaatg atgctattgt agaagctcca aattttcttt ctaagattaa
     6001 tggaatatat aacgataaga atgaatatat taagttttta gatttattaa gagattcgga
     6061 aagttcaaca tttattgaat ttaatggaga tgtagataga ttttcaaaag cttttacaag
     6121 agaagctttt agtttatcct ttttattgga ttcaaaaggt gcaatagata aaaactctgt
     6181 tttaaataat aaagattatt ccatatcaac actagcattg aatcaacaaa ttatgatttt
     6241 agagaaagtg gaagagttgt tagaaggaag tatgtttttg aatggtttaa gtggagaaga
     6301 caagataaaa ggtctattta cagtacttca catggataag aaatttgttc atatgatgaa
     6361 caatttcgat tactcactta taaacccaaa attgattata tacatgtaca agagtattgt
     6421 atttgataaa gaaatagttt ttcttatgct tttacttagt aagattggat ttgatattat
     6481 catattatgt ccaggaggag aaaacaatat agagaatgtt ataaataacc aattgattga
     6541 tgtacacaga ttggataaaa tggtatatga ccttaaatta aattcgcttg aaaatgatat
     6601 acctttactt aaaaaaattt ttggaaagag gagacgattt tagatgggga taaatataaa
     6661 tagtttttca aaagataaga actctgtaga ggttactgaa aatttgccta cagcagttca
     6721 agaagaaaac ttcgatataa tggaatatac aaataataaa aaaaatgaac tgagaaagtc
     6781 taaggaagtt gaggcattga catctcttat agaagtagaa aatccagata cgattttaca
     6841 gtttggtagg aaagcatctg aaggagtagc aagagtttct gatagccttt taaatacaat
     6901 aaaattaaat agaaatgaag aaaattctaa aatgttggtt catttaacta aaataatgga
     6961 caagtttgat ttagacgatt ttcaagaaac taaggaacca aatttcgttc aaaaactttt
     7021 caaaaaagct aataatgcca ttgaaatgat gtttcaaaag tatgaaacat tgggaggaga
     7081 agttgagaaa attcaaattg agcttgaaca atacgaaaga gatatagcac tttctaataa
     7141 gcaaataggt gctatgttaa atgaaaactt tgagttttac aatgaacttc aaaaatatat
     7201 tgttgctgga gaaatggcaa tagaagaaat ggataatgaa atactacctt tctttaaaca
     7261 aaaatcagag actagtggag accaaatgga tgttgtcaat tatcaagaac tattaaaagt
     7321 ttatgatatg ttaaatcaaa gagtctatga tttaaggata gcagaaaata tagctatcca
     7381 aacaatacct atgcttagag gtatgcaaca taataattat ggtttaatta gaaaaataaa
     7441 tagtgcattt gtcgtaactt taccagtatt taaacaatgt ttatctcaag ctatattact
     7501 taaaaagcaa gaattacaag caaaatctct aaaagcactt gatgacaaaa ctaatgaatt
     7561 attgcttaga aatgcacaaa atgtaagtac tcaaagtgca caaatagcta gaatggctgg
     7621 aacaagttct gttcaaatag aaacattgga aaaaatgtac aatactatca agtctggtat
     7681 agatgaaact atgagaatag aagaaaacaa tagagcttta attaaggata atacaaagag
     7741 attggaagaa ttaaatacga ctataattta taataagtag tattatatta agggctaatt
     7801 tgtataaagt tggccctttc tatgtatatg aggaggctag acattgaaat atttcgatta
     7861 tatctgcaaa gatgatttgg agagaatttt tttgaaggag ccagaagatt ttagtgctaa
     7921 gactgaaaaa gatgtgttga aatatgcact gggcgctttt ttatacgttc cagctactca
     7981 atataatatg atttataaga gtattatagg ggatgttaag ggagttagac cattagctat
     8041 ttgtttagaa gatgctgttg gagtaaatgg agagttggag gctatagaaa atcttagatt
     8101 aattttaaaa aatattagca atgaaagtat aactaataaa gatggaatac cactaatttt
     8161 tgtaagaata aaagatgtgg agcaactgtt gagaattaaa gagattataa ttaaaaatag
     8221 tcattttata acaggaatat taattcctaa agcaaattcg gagttaattg aaaattgtat
     8281 agaagcttta gattttatga atcttcaaga tatgtatgtt attccgatag tagagacaaa
     8341 agaatttata tataatgaaa aaaaagaact tagttttact aatttgtaca atgctatttt
     8401 aagacataag tcaagaatat taagcattag agtaggcata actgatatat tagggatgta
     8461 tggtataagg agagataaaa acttttctat atataataat ttgatttgtt cttcatttat
     8521 attagatata atcacatatc ttcaaagacc agagttagat atacctataa gtggtggagt
     8581 atcagaattt tttgatatga cgaataagga tataagaaat aagtatatag aagaaatatt
     8641 attggataaa tatcatggat taattggaaa gaccgtaatt catccaatgc aaattcaaat
     8701 tgtacaagcg ctatctacag tatcatatga agattttact gatgctttgg atatactgga
     8761 tagtacagac agtaagtatg gtgtgagcaa gggtgtatta ggagagagaa tgaatgaaac
     8821 aaatccacat tttttatggg ctaaaaaaac attgattcta tctaaaatat atggtgtatt
     8881 aaataaagga gtagactatg aagaattgct taaattttaa ttgtgagatt gacattattg
     8941 ataatccatt gaatttagaa ctaaatgatt ttttagacgt aggaataaga aataatagca
     9001 aaagacgttt tttgtttatc tcaaagaaac tcggtaaaca tttagcatgt aagccaagtg
     9061 agatggataa tttgggaaaa ttaatggcta caatttataa tgaaaaaaat aaggaagaat
     9121 caaatggtac tgttatagct tttgctgaaa ctggtacagc tgttgggcat agtttttttg
     9181 attacttatg tggagattat gaatttattc atactactag agagcagttt gacgagttag
     9241 aaaatctgga atttttagag gaacattccc atgcaacaga ccagaattta tactttggta
     9301 tgcttaacaa ctttaaattt ggagatgaga taatattggt tgatgatgag attaccacag
     9361 ccaatacatg catgaatata attaggaaaa ttcaaagcat ttatcctaaa aagaaataca
     9421 ctatatgctc aatactcaat tgggtaagtg atgaaaattt aagtaaagtt actcgtttag
     9481 aaagagagct agattgtaag atagattttg tatacttatt taaaggaaac tttaaatttt
     9541 atatagatga agatattatt tcaaattaca acgataatta tagtattact aaattagaga
     9601 ctatttcaag tgataaagta aatttagtag taaatcatat ttatgttgat ttagaagatt
     9661 atattggaaa taagaaatat gtaaaatata caggaagatt tggaattaat agaagagaac
     9721 aatttagatt aaaagaaatc gttaagagag aaagtaaaaa aataactatg gccagtaaag
     9781 aatctttaaa aaatgaaaaa gaaaagattc tatttttagg tactgaagaa tttatgtata
     9841 tacctatgct gtttgcaaaa gaaaaagaaa atcaatatga tatatactat cattcaacaa
     9901 caagaagtcc aattattaat attgataaga ataattatcc tattaaaagt aaatttatgc
     9961 taaagagttt ttataatata gatattcaaa attatattta caatatagat aaacataatt
    10021 tttctaagtg ttatttattt gtagagctga ataaaagtga aaattcttat ctagaattta
    10081 ttgatataat taaaaaaaca agtataaaag aattaactat agtatgttgt agctaaagtg
    10141 ttttaacagg ataaaatata tcacgatttt aagataggag ttgagtcata tgagagattt
    10201 tagcacgtat tctaaagatg atgttatttt tttattaaaa gatatatcaa atttaattga
    10261 agaagaagga aataaagaaa gagaattaaa aatacaaaat ggtagacatt attcagaaat
    10321 gataccaata gagtatgaag ttagtgaaga ttatctgaat ttatatcatg agaaactaag
    10381 agaaaataaa gataaacttg cattttctat aggagttatg tgtgaaaaaa taattaaaaa
    10441 aaatggaaat gatgtagtac tagtttcact tgctagagca ggaacaccta ttggtatttt
    10501 ggcaaaaaga tatataagaa gcaagtataa tataagttta cctcactata caatctctat
    10561 aataagagat aagggaatag atatgaatgc aattgaatat atagtaaaga atcatccgag
    10621 ctcaaaaatt caatttttag atggatggac tggtaaggga actatatcaa aagaacttga
    10681 aaaagcatgt gatgaactta atgttaaatt taaaaaatat tttgactcaa cacttgctgt
    10741 attagctgac cctgctggat attcaggact ttatggaatt agagaagatt tcttaatacc
    10801 aagtgcatgt ttaaattcaa cagtttctgg tttagtaagt agaactgtac taagagagga
    10861 tttaattggt aaaaatgact tccatggagc taagttttat agacatttaa aagataaaga
    10921 tgaatctatg aactatatcg aaactataga agaatgtttt aaaaatcaat ttaagaatat
    10981 atctgatgaa gttgaaaact gggaaagtga cattataaca agagatggat actttgatgt
    11041 tttaaatata aaagaaaagt ataacataac agatattaac tttatcaaac caggtgtagg
    11101 ggaaacgaca agggttttac ttagaagagt tccgtataag attcttgtta aagacttaaa
    11161 tgataaatct ttagaccata tattcatatt agctaaggag aaaaatgttg aagttgaaca
    11221 aatggattta aaagcctata aatgttgtgg aataataaaa aatatgaaag atatttagga
    11281 aatatattag acaggagaat attgaatgat tgttttttca gatttagaca ggagtatcat
    11341 ttactccaat aaatttttaa atgctgatag taaatatact aatatagaaa tttataggga
    11401 aaaagaaata tcttatatat ctttagatac tataaattta ataaagcaaa tacaatatta
    11461 tggtatgttt atacctacaa ctactagaac agtggagcaa tttaagcgta tagaatttaa
    11521 taaatatggg atttattttc cttggagtat aacttcaaat ggaggagtta tacttaaaga
    11581 taatgaaata ttgaaatcat ggagtgaaaa aatagataaa ctaaaatgtg attatgagcc
    11641 cattgaaagt atgattcata aatttaaaga ctatttgaat gtagatggaa taactaattt
    11701 taaagttgca gaagatacat ttttttatat agttgttgat ttatcaagat ttaatttgga
    11761 ttcaatcaaa gaatatacaa atatattgga atcaaaaaat tggaagtttt atgtgagtgg
    11821 cagaaaaata tattttatac caaaagaaat ttcaaaagaa aatgctataa aatacttgac
    11881 taaagaatta ggaatagaat atttttatgc agttggagat tctattatgg actatggtat
    11941 gcttaatatc tcaaataaat cctatgtatt gaagcatggt gatataaata aaaatgaaat
    12001 agaaaattca tttatatcaa gttctttcaa tggaatgagt ggaactgaag agatactatc
    12061 taacatttta gacgagaact gcctacttaa tatataatga ctgatatata aataataaac
    12121 tagaaaaatc tgaaataaat tatagtaaag tttatcatac taatttattt cagattttta
    12181 tcaatttata tttaagtaag aactcaatat gttaaaatga aggtggagtc attgcccaga
    12241 ctgagataca tacctcatca tcataatttt catacacatg tggtatagta ctgttaaaat
    12301 aaaaactatc tccttcctcc aatagatatt ccttatttcc aagtgttatc ttcattttac
    12361 cttttattat atagccacat tcttctcctt cgtgtgtcac aaattcactg tttttttgtg
    12421 tatgaccatc tagtgtaaca agtataaatt ctattttttt cccagataaa ttaggaacaa
    12481 gaagttcata aaattttgta gaatcattag ttactatttt ttttcgatta tttttcttta
    12541 ctactatatc agagtcttct cttccaatct cttcaaaaaa ataacctata tttatatcaa
    12601 gagcttttga tattttccac attgctacaa tagatggaac aaccatatta cgctcaattt
    12661 gacttattaa gcctgtactt aaatctgaat tttttgctaa agtagagata cttatatttt
    12721 tagattttct aagagatcta attttgcttc caacatctat attcattaga tacaaacccc
    12781 ctcagacatg attataacat ataatttata aagattaata aaataaaaaa aaagttttac
    12841 aatacaagta ttttttatgc tatattgaat tttctgaaaa gatgcattat aatagtatta
    12901 atattcaata tattgaatat tgataacgat attttaattt aatgaaatgg aggtaattaa
    12961 gatgaagagt tttgtaagtc ctgataatat ttgggtatta tgggcagttg taactggatg
    13021 ggcagcattt agtatttatt tggaacaaaa gtacaattgg gcatcaaaag tgtcgggagc
    13081 aataatagct cttgtaggag caatgttatt atcaaatcta aatattatac cagtagagtc
    13141 tgttgtatat gaccaagtat ggggatatgt tgttccttta gccatagctt tattgttata
    13201 tcaatgtaac attaaaaaga tatggaaaga aagtggaaga ttgctcatta tatttcttgt
    13261 aggttctgta ggaactgtac ttggagctat gattggattt ttagcattaa aaaatgtagt
    13321 accagattta aatattgttg cagctatgat gacaggttct tatataggtg gaaatgtaaa
    13381 ctttgctgct atgtcagggg cgtttgatgc gccaggagaa ttagtatcag caacagttgt
    13441 agcagataat cttttaatgg cattatattt ctttgtctta atagctatac catcaattgg
    13501 attttttaga aaacatttta aacatccaca tgtagatgaa atggaaagta taggtataac
    13561 tgaaggagaa acagtagcag caaattattg gggaagaaaa gaaatatctt taaaagatat
    13621 agcaatagcc attggtagcg cctttataat tgttgcagta tctgtagaac tatcaacttg
    13681 gtttaaagct ataataccat tatcaaatcc ttttttagca atgttaaata ctttatttgg
    13741 aaatcaatat ctaataataa ctactttgac tatgttgttt gccacattta tgcctaattt
    13801 ctttggtgat ataaaaggag cacaggaatt gggtacattt ttaatatata tattctttgt
    13861 tgtagtaggt gttcctgctt caataacatt aataattcaa aaatcaccat tactactttt
    13921 atactgcgga ataatggtac taataaacat gctagttaca tttatagttg caaaaatatt
    13981 taagtttagt ttagaagaag ctattcttgc atctaatgca aatattggtg gtcctacaac
    14041 agcagcagct atggcaatat caaaaggatg gtctaagcta gtagggccaa tacttatagt
    14101 tggaacattt ggatacatag tcggtaatta ttttggtctt ttagtaggaa atattttgat
    14161 ataattttac gtgaaaaaaa gttgtttatt agaaagattt ttatttaatt ggaggttgtt
    14221 atgattttcg atggacattc agatacttgg gcagatgtaa caataagaag gcttaacggg
    14281 gaaaaagatg tatttagaag aatccatctt gataatttta aaagagcaaa tatagaagga
    14341 gctatttttg taatatggat agacccacca tatgataaag agcctgaaaa aagagttaaa
    14401 caaattataa gttctataaa agatgaaatt gaagatatga aagatgttgt aaacatagta
    14461 aaaaaatatt cagatataga taaaggtata agtgaaaaca aaataaatat tttaattggt
    14521 cttgaaggat taagccatat tggaagaaat atagatttac tagattattt ttataaagaa
    14581 gttggtgcaa gacatgcatc attaacttgg aatgaacaga atgaacttgc tacaggatgt
    14641 ttaggggatg aaaatcgtgg tttaagtgaa ataggcaaaa aagcagtcaa taaaatggaa
    14701 aaattgggca tgatagtaga tgtgtcacat ttaaatgaaa aatcattttg ggatttaatg
    14761 tctgttgcaa gtaagccagt tatagcttct cattctaatg ctaggagttt atgtgatgct
    14821 aaaagaaatc ttacagataa tcaattaaaa gaaatttcaa atacaggtgg attagttgga
    14881 ataaatagtt ttagaggatt tactaatagt aatcctaaga agcaagattt aatacatttg
    14941 gttgaccata tagactatat ggtaaatctt ataggtatag aacatgtatc ttttggtttt
    15001 gattttagtg attacttagg gaaagagact ataggttctt ttactggaaa tgctagtgtg
    15061 ccaagtgtag atggtcttga aagcacacat acagcacaga acatagtagt agagcttgaa
    15121 aatagaggtt ataaaaagaa agatatagag ttaatctcta gtggtaattt ttataggctc
    15181 tttaaagata tactaaaata atataataaa gaacttaaat ttataaaata gtatatttat
    15241 acaaataata tctacatatt taaattggcg ttcataattt attaaatttt aatgttacta
    15301 gaagtttcaa ttctagtaac attttttatg ttaaaaacag atatagatat gtattgacaa
    15361 tgataattat tattgataaa ctataaaagt gaaaatgata ataattttca ttttaattaa
    15421 taaaagattt ttaaggaggg agaatgaaga aaaggtattt agtcatagga ataataattt
    15481 tgtcttattt ttctttattt ataggagctg aagatataaa tataatgcat atatttgcca
    15541 aaaatcagca taaacttatg atatttatca tgagtagaat accaagatta ataagtatat
    15601 tgattgctgg tgtaggaatg agcatagcag gtcttataat gcaacagata agtaagaata
    15661 aatttgtatc acctacaaca ggtgctacaa tagatgcagc acaatttggg atagtaatat
    15721 gtatgctttt agtaccaact gcctctattt ttacaaagac aataatagca tttgtatttt
    15781 ctcttgtagg gacttttacg tttatgaaaa taatagggaa gttacaattt aaaaatataa
    15841 tctttgtacc attagtaggt ataatgtttg gaaatataat agggtctatg acggatttta
    15901 tcgcgtataa atacgatttg agtcaaaatg ttagttcttg gatgcaaggt gacttttcta
    15961 tgatacttaa aggaaattat gagatattat atatcacaat accccttata atattagcat
    16021 atatttatgc aaataaattt actgttgttg gtatgggtat ggattttgcg acaaacttag
    16081 gtcttagtta caaaagaatt gtcaatatag gtctgataat agtagcactt gttacagtat
    16141 gtgtagtcgt tacagctggt aatatccctt ttataggtct aatagttcca aatatcgtat
    16201 ccttgtacat gggtgataat ataagagcaa gcatatggta tactggttta cttggagcga
    16261 tatttgtact tatatgtgat atttttggaa gaattattat ttatccatat gaaatatcta
    16321 tagggcttac agttggagtt ataggaagta tactattcct ttatttaata ttaaggagga
    16381 atgtgaatga agcttagtct caatcaaaaa atttactttg taagtatatt actattcata
    16441 tcaatggcac tttttataat ttatggaata gacatgaatc atttagaata tgcattatca
    16501 caaagaatac caaaaatatt tgctatggta ttaggtggag gatgtatagc atttactact
    16561 gtagtgtttc aaacaattac aaacaatcaa atattaacac caagtgtact aggtttagat
    16621 tcattgtatg taatgattca aactataata gtattcatat ttggttcaag cagtagtctg
    16681 attataaatg aaaattataa ttttattatt aatgtagcta tgatgattgg agcttcattg
    16741 ttattatata agacgttatt tgaaaaaaat aaaaataata ttttcttttt aatcctagta
    16801 ggaatgatat ttggaacctt atttaaaagt gcaacaacat ttattcaggt aatgatagac
    16861 cctaatgaat ttttagcttt acaaactagt ataatggcaa gtttaaacaa tataaacact
    16921 aatgtattgt taatagcatt tataataatt attgccataa tacattttat atatgatgaa
    16981 ataaagtatt tagatgtatt gtcgttaggt aaagaacaag caattaattt aggtgtagat
    17041 tttgataaag cagttaaaaa aatgataata ttaatagcaa ttttagtctc aatatcaaca
    17101 gcacttattg gacctatgac atttttagga cttttacttg ctaatataac aagagaaatt
    17161 tttaaaacat ataagcatac atacttgatt tcgggttcta tgttgatagg gatgattaca
    17221 ttgataatag gtcaattttt tatacaacat gtatttaagt ttgatacaac tttaagtgta
    17281 gtaataaatt ttataggagg aatttatttt attcaattac tattaaaggg ggcaaataga
    17341 tgatagagat aaaaaatata tttaaaagat ataagaataa aaatgttgta gatgatgtct
    17401 cttttagtat agaaaaggga aagataacat cttttatagg accaaatgga gctggaaaaa
    17461 gtacagtact atcaatagtt acaagactta taggaggtga tggtggagaa gtaataatag
    17521 aaggaaaaag cttgactaat tatagcaata aagaattagc aaaaaaaata gctattctta
    17581 aacaatctaa caatataact ttaaagctta caataagaga attagttgga tttggaagat
    17641 ttccatatag tgaaggtaat ctaactaaag aggatgaaaa ttatattgat gaagcaatag
    17701 aatatatgaa gttgactgat atacaacata agtatttaga tgaattgagt ggaggtcaaa
    17761 gacaaagagc ttatatagca atggtaatag ctcaagatac agagtacata ttacttgatg
    17821 aaccactgaa caatttggat atgaaccatt ccgtacagat gatgaaggta cttagaggtc
    17881 tttgtgatga attagataag actatagtat tagtaatgca tgatataaat tttgcatcat
    17941 gttattcaga taatatagtt gcattaaaaa atggaaaagt tgaaaaagta ggtcgtacag
    18001 atgagatagt aaatgaaaaa gttttagaag atatttatga gatgaatttt aacattaaga
    18061 atataaatgg aaatcgaatc tgtatttatt tttaaaatat tgtatatgaa aataaacttg
    18121 ctatatgatt ttttataaag tttttaaatt aaaataaaga ttaataggag agaggaaaat
    18181 attgatatga ataaaaaagc agcaatagta gcagcagtag ctattatagg attagtgaca
    18241 gtgtttgcat taggtggaag taaaaagaat gaatcaaaaa catcagaaaa ttcaaataat
    18301 actatgaaga taactcataa cttgggagaa acagatgtaa aattaaatcc taaaaaagta
    18361 gtggtatttg attattcggc tttagataca atggatgcat taggtgtagc tgagacttta
    18421 gtaggacttc caaaagcgag tttaccagca tccttagaaa aatataagga tgacaagtat
    18481 gcagatttag gaggattaaa agaaccagat ttagaaggta taaagtcggc aaatccagat
    18541 ttaattataa taaatggaag acaagaagat ttttatgagc aattgtcaaa aatagcacca
    18601 acaataagta caagtaaaga tgataagaag tacttagaat ctgttaaaaa taatatagac
    18661 aaaatagcaa aaatatttgg agtagaagaa aaagcaaatc aagaatttag taaaattgaa
    18721 aagaaaatag aaactttgaa taaaaaagta acagataaaa atttaaatgc tttaactata
    18781 atggtaaatg aaggtaattt aagtgtattt ggtgaagaat caagatttag tatattatac
    18841 aatagttttg gatttgaaaa taaagataaa aatataaaag aatcaagtca tggacaaaat
    18901 attacttttg aatatatagc taaacaaaat ccagaagtta tgttcgttat agatagagga
    18961 atagctactg gcagtgatgt aaaagaaagc tcaactgcaa aatctgtttt gaacaatgat
    19021 ataataaaat ctatggatgc atataagaat gataatataa tatatttaga ttcaccaaca
    19081 tggtatgtaa atgatggtgg tctaacatct ttaaataaaa tgatagatga tgcttcaaaa
    19141 gcggtaaatt aataatccga tataaccaaa atgtattttt tataaaatga ttatcaaata
    19201 aactaggagt ttttaagaac tcctagttta ttttttgcat aaaaaagata tagtttaaac
    19261 aataatatta tctataataa gataaatcta ttcaaaatct aataaaatat gaagttatac
    19321 tagacaaaaa gaataactct taaatataat taagttatat aataatatat aattataatg
    19381 atataatttt aataggtggt ttgtaattaa aaagtttata aattatatta atagtaataa
    19441 tctataagaa cttatatatt agaaaaaaat ttttgtatat ataaaggact ctaaagatga
    19501 actatgtaaa acaaggttta tatattctaa ttgtatagaa aaatttattg taaacgcatg
    19561 tatattatta gaaaaaatag tttggaggta ttcaataaac ataaaaaaca atatagggga
    19621 taaaggtgtt ttatgaagag atttttgagg agaattatct tagttttatt tattttgttg
    19681 atttttataa gttttatttc tattaaatta attcataatg ttggagatta tggaaaattg
    19741 attaattatg ttgggattgt tcgtggagct agccaacgtc taactaaact agagatgaat
    19801 catcaaccaa atgatgaatt aatagaatat attgatgaaa tcctacagga gttgattaca
    19861 gggcatggag attatggttt agtaatcact gattgtaatg aatacaatga ggacttactt
    19921 ttattagaaa aaaagtggga agatttaaat attgaaatta aaaaagtaag aatgaaagaa
    19981 cagaataacc agttattaag tataagtgaa gagttttttt ctcttgcaaa tgatacagtg
    20041 tttaaaatag aaaacttttc taaagaaaaa agtaattact taatgacttt aataataatt
    20101 atatctatta taggtatatt agcatgtatc atattgattt tacaatatag taaaaaaatg
    20161 gttagactgg aaaaattaaa tgtagattta aaaaatattg catataaaga tgaactaaca
    20221 ggagtaaata ctatagaaaa atttaaattg gatgcaaatc aaaatatttg tatacataag
    20281 gataagaaat ttgctgtatt ctatattgat tttgaaaact ttaaatacat taatgatata
    20341 tttggatatg attatggtga catgatactg aaaagatatg ctaatcttat gatgaatgac
    20401 atagggaaat atgaaatatt tgcaagagaa attgcagata gatttgttgc gttaagatgc
    20461 tatgtagata aggaagattt agtggtaaga caacgagatg tagacaatga actaattaat
    20521 ataacaaatg aaattaaaaa taaacatagt attactgttg ttagtggaat ttgttgtatt
    20581 gaagatgtaa atgaaaaatt aagtatagat ggtttaataa atagagcaaa ttttgcacaa
    20641 aaaactgtta agaataaacc aggtactaat tatgcttttt ataatgacag tattcgtaaa
    20701 aaaatgattg aagaaaatac tatcaaaagt agaatacatg aagctattga aaaaagagaa
    20761 tttatagttt atttacagcc taaagttaat ttacatagtc aaaaaataaa ttgtgcagaa
    20821 gctttagttc gttggcttac tccggataaa ggtattatat ctccagcaat atttattcca
    20881 gtacttgaga agaatttttt tattgcctta gtagataagt atgtttttga ggaggtttgt
    20941 aaatggatta gaaagaggtt agatgaaaat aaaccttttg ttcagatatc agttaatgtt
    21001 tcaagaatac aattttataa cactaaattt gtagaaactt attccaatat acaaaataaa
    21061 tacaggatac ctaaaaatac tatagaaatt gaatttacag agagtgttgc ttttgagaat
    21121 caaaaacatc ttttggaaat tatacatgat ttgcatgaaa atggatttac ttgttcttta
    21181 gatgattttg gaaaaggtta ttcgtcttta agtgttttaa aagatttacc atttgatgtt
    21241 ttaaagcttg attctatgtt tttcaaggct agtttagata aagataaaga gaaaatagta
    21301 ataaaaaata ttgtacatat gttaaaagaa cttaatatca caactgttgc tgaaggcatt
    21361 gaatgcaagg aacaagttga atttcttagg gatattgggt gtgatttagt tcagggattt
    21421 gtattttata aaccaatgcc aatattagaa tttgaaaaaa tattggataa agagtttgtt
    21481 tataatagct aaaatgtact ttataaaata tgaactaatt aagtaaaatt attttgtact
    21541 gaataaattt ttactttatt tacagaataa tgattattta cagaaaagcg cgaaaagtgt
    21601 attcctttaa gtgtgggatg gatagcacaa ataggggaga aaaaatattg gccatataag
    21661 aatgcataat aatataaata tttgaaaggt cagcattata taaaaattaa aaagaaaata
    21721 aataattttt ctataaaaaa gagactatga gaaaatttac tcatagtctc ctaaataatg
    21781 tatttatttt cttcctgcaa cccattttaa tccccaatga aattcagtat ccatttctct
    21841 atgacctttg aagtatttaa ctactttttc aacactgaat gtctcatcat ttacattttc
    21901 tatcaatgct attgaacaca tattatagcc atttttatgt tcatcaagtt tgacaactaa
    21961 gtctgaatct tgtttttgtt tgattgttac aattccatca gcttcagacc agttagctac
    22021 accttcgtat ataaaagcat aaataagtat tctttttata tcttttattt tattaccatt
    22081 tattcttaaa ttttctcctt gtgtattact tcctgttctg tcatctccat ctaattgagc
    22141 aaaaggtgga ttgtctaaac taccaaaggc atttcctaat gcttgaacag caccttttac
    22201 accatttttc atctcaaata ggcaacccaa atctaaatct atattactgc ttcttaaaga
    22261 gccaaaaaaa cccttttttt gtactttttg attccaattt aaattaacta agatttcacc
    22321 taaatcgcta ttttcctttt ttgtaaggtt tattttttgt ccctttttta attctatagc
    22381 catagtaatc ttcctccaca aacatagatt tatttaaatg gtagcactta ataagtcaat
    22441 attcaacaag attagaataa taatattaaa attatattaa atatactaaa aagtaggaat
    22501 cacaatacct ttgtattttt cttcaattaa gcttcttgct ttatctgatg taagaacatc
    22561 ttgtagaact ttaatctttt cactgtcttt atcttcagtt ctaaatgcta aaagatttgc
    22621 atactcgtca tttataggag ctgtataaat tccgtttttc ttggcatcaa gaccagcagg
    22681 tacagcataa gtagtatcta taaatgcagc agtcacatct ggaagagaag atggaatttg
    22741 agcaatatct aactctttga attgtattcc ctttggattt tctattatgt ccttaggact
    22801 aactaatcca tcccctttag gtaacgttaa aagtccaata ctttcaaggt atctaaggtt
    22861 cttagaacaa gaagatgggt tattgcttat tgcaattgta tctccttttt taaattcatc
    22921 aacagatttt atcttataag agtaaagtat tgctggacat acatatagtt ttttaccagc
    22981 cattattttg taacctttgg ccttaacagc ttcttttaga taaggttcat gttgaaatag
    23041 atttgcatca atttcaccat tatttaaagc ttcatttgga agtatgtaat catcaaagtt
    23101 tttaacttct aaagtatatc ctttttcttt aataagtggt tttaattctt ctaataactc
    23161 tcctcctgga actaaagtag caccaacaac aattttttta tcatctttag ctttagaaca
    23221 tccaaccaca gctaaagtta atactagaca taataacaaa cataataatt ttttgaattt
    23281 catataaatt ccctcctgaa tataattgat tagttaaaaa taaaatacca ttctttatca
    23341 cactgcaaaa taaaaataag cctgagttta aactcaggct ttaaaagcta atttagactc
    23401 atctttcagt ataatactgc aggatttagc accaatgtat atagaaaata tactggttgc
    23461 tgggtttcat agggccagtc ccttcaccac tctggataag aagtatcaat ttttaattat
    23521 gttataaata ttatcatgat tttttacgtc tgtaaacata attttataaa tattttaaaa
    23581 tctttaatgt attttattta acacctaaaa ttccagaaac tagaatatca gtatcagcac
    23641 ctaagaaata gtcatttatt tcaatagaat ccagagtatt tctcaaagca ttttcttcat
    23701 gccttatacc aataagttta ttttctacaa atgcaacatc acattttcca aaaaaatctc
    23761 caaaaaattt tatactagtt attatgcctt tatcaacatt taaattaaac tcaacatttc
    23821 caccagaata ttttaattca tttgaaagag tatattttgg tgaattacca aaattccaat
    23881 cccaagtttc gtattttgat ttagtaagct tttctatttg ttttatatca tgctcactca
    23941 aacaataatt agtattatca gtatttgttt tagaaagata atccattata gcatctttaa
    24001 attctaatat atccattttt gaatgtaaat gttcacttat attggttaca cgagatttta
    24061 cagattttat tccttttcct tcaaatttta taggtttaac ctttaatgct tttgagaggt
    24121 catttatttc agatgaaaat aaaagagtcc catgatgcat tactttgttt ttgtagttgt
    24181 attgagcatt tccagaaaat ttcttgccat ctataagaag gtcatttctt ccagaaaatt
    24241 cggcattgac atctagtgtt ttaagtaaat ctattatggg ctgagtgaat cttctgaaat
    24301 cactaaaatt attattgtta caagctataa atgtaaaatt tatatttccc aaatcatgaa
    24361 atacagctcc accaccagac tgtctcctaa cgacaggtat agaatattct tttatatatt
    24421 ctaaatttat ttcagaaagt gtgttttggt tttttccaac aattatagaa ggttcattcc
    24481 tccataaaat aaataagtct tcatcaaaat tttttagaag atattcttcc attgctaaat
    24541 taaaataagg gtttgttttt tcattataaa ttaataacat taaagtagtt cctccataga
    24601 attgataaaa tttttattat gaatacaatt aattttataa ttcgacaata atataaaaac
    24661 tcctttaaat tagaaatttt aaaaactacc ttgcaaagtt taaataaata atatatactc
    24721 aaaatatgtt taataaaagt gaatagaagg gtagaggcgc aataatctat cagtactaat
    24781 aaggagttaa gcacagagat ttattaggaa aggagatatt gccgaagttt tataatcgat
    24841 gctttaagat tatattgctg ggggtacata gaaaatatgt acaactgtca caggtttatt
    24901 tgtggtgagc tatcatttaa ggaattatat tatatttatt tttactattg ttgatatatt
    24961 gtaatgttta agccttgagt gtttaccact caaggctttt ttagttttat tgtaacataa
    25021 taccctgtac agttcacaaa attaaaagga gagggagatt tatatgagga acaaaaaaat
    25081 taatattatt tttctgacga caatcatgtt cataatgtca acagtaatgg tttttgcaga
    25141 agaagatata gacactattg cactagccaa tgcagaaaaa tttgggattt taactttaat
    25201 accaccatta gttgctataa tacttgcatt tataactaaa aacgtaataa tttctttact
    25261 tataggtatt ctttctggta gttttataat taaagctagt ggtattaatg tttttgcgac
    25321 atttatacag gcatttttag atttagtaga cagagcttta gtatctttag cagacccttg
    25381 gaatgcaggg ataatactac aggttttggc aattggagga gtaataaatc ttgttgctaa
    25441 aatgggagga gctaaagcta tagctgaagc acttgctaaa agagcaaagt cagcaaaagg
    25501 gacacagctt ataacatggt ttttggggct tttagtattc tttgatgatt atgccaattc
    25561 actaatagta ggtcctatga tgagacctgt tgcagataaa atgaaaatat cgagagaaaa
    25621 gctggcattt ataatagatg ccacagcagc accagttgct ggtttagcta taatatctac
    25681 atggataggt cttgaggttg gtttaataca tgatgctttt gaaagtataa gtatagatgt
    25741 agatgcattt ggtatatttt taaatacaat accatttaga ttttacaata tattaatatt
    25801 agctttcatt gtaatatcag cattattatt aaaagaattt ggtccaatga gaaaagctga
    25861 gataaaatct agaagtagaa aaattagtat agaccttgat gaaggcgtag aagagttgga
    25921 tgatttagct ccaaaaaatg gagtgaaatt gagtgtatgg aatgctataa tcccaatagg
    25981 aacattgata atagttgcat tagcatcatt ttattatagt ggatatacat ctataatggg
    26041 aggagacgat aaggctctga tacaactatt tacaaattct ccatattctt ttgaagcaat
    26101 aaaagaagca tttagtgcat ctgatgcatc tagagcatta ttccagtctg cacttgttgc
    26161 aagtttagtt gctattataa tggctgtagt aaaaaagatt tttacaatat cagaagcaat
    26221 tgatgtttgg atagatggaa tgaaaagttt agtaatcact ggtgtaatat taatacttgc
    26281 atggtcatta agttcagtaa taaaagagtt aggtacagct aaatttttaa tacacctatt
    26341 atcaggttca ctaccaccat tcttattacc aagtttaata tttggtttag gagcaataat
    26401 atcttttgct actggaacag cttatggaac aatgggtata cttatgccac ttgcaattcc
    26461 attagcatac tcattaaatc ctgatatgtc ttatgtaata gtaagtacta gtgcagtttt
    26521 aacaggtgca atatttggag accattgttc tccaatttca gatacaacaa tactttcttc
    26581 aatgggagct ggctgtaatc atatagacca tgttaacact caaatgccat atgctatatt
    26641 tacagcagta atcactattg tatttgggta tataccagct ggacttggat tgccaatata
    26701 tatagtatta ccagtggcaa tagctgctat atttgtaggt attcaaataa ttggtaagaa
    26761 agttgatgaa gcggaaatag aacttgtaga ataaagatat aaataagttt caccttttat
    26821 tatcactatt ttgttagttt ttagataatt ttaaaaagtt attgaaaata atggcgaaat
    26881 atagtaaaat aaaagagtga taagcggatg tggcggaatt ggcagacgca ctagacttag
    26941 gatctagcgc cattggcgtg ggggttcgac tcccttcatc cgcactttac agtacttaat
    27001 tgtactgaaa aaaactgggg ttgctggctt tcggagtggg ctatcccgtt tttatttttt
    27061 tgtatatata atagattggc actttagtat aaggtgcttt ttttataggc aaaaacagta
    27121 catttatttc tgttttatta tcattcatct attagaatat actttagtta agaatgtgtt
    27181 aataagcagt gttcacattc ttgataaaat gtggaataat atgtattttt actaagacgg
    27241 aaatgaaata attaaaccat taaataatat tataagatga tgaataagta gataaatcaa
    27301 attttgtagg aggatattat ggaaaaatat atatggttgt ttccattact ttttattttt
    27361 catgatatgg aagagataat aggttttgga atatggctta aaaaaaataa atcaatgctt
    27421 gataagaagt atccatttat cagtaaagtt tatgaaaatt atagtactga aggcatggca
    27481 tttgctgtgt ttgaagaatt tattttatgc attattttct gtatacttac agttataaca
    27541 gagaatcagt atgtatatct actatggtta ggtagtttta tagcatatac cttacatttg
    27601 gtaattcata tagggcagtc aatcataata agaaaatata taccttcact tattaccagt
    27661 atcatatgct taccaataag catttggtgt ataagtaaga gtatttatat agttgattgt
    27721 gaaatgagta caacaattct gtacagtata attggcatta taattgtagc attgaattta
    27781 aagtttgcac agtcgctgat tggaaaattt acaaagtgga tgtcaaacat ataaatttta
    27841 gtttattgga cagttcaagg aatcagaaat tggaatttta tagtattaat attttatagt
    27901 attaatataa tgtataaaat tttatagcaa tgtatttcat aagtataaag tgactagaat
    27961 tttaagaaat atacaaattc tataaaaact aaataatttc aagtatattt taaaaatctt
    28021 atttttcaca agtttaaaat tgtaaacgat ttcatcaagt ctatgaatat acttttaaca
    28081 tgttatagta aatctcaata taaatttaca tttggttctt tgtgtgttat tttaataata
    28141 aattaagtga aaatgttatt ttacattaat atataatatc tttgcataag agtgaataat
    28201 ataaaagaga aataattgat aataaaaact taacaaaaaa attaatacat aaaaaaaaat
    28261 aaatataatg tgttgacatt taattgaaat attcatacaa taaaattgaa aataaatatt
    28321 tccaaatgag attagcgaga gagtccttta tacgaaaagg cgccgaagaa gaaatttaag
    28381 aaatgccaat ttttagagaa gctttcaggc aaaaatatcg ctaattgatg ggactctgga
    28441 gagacttaag ttaagatact ataagtcacc gaagaagcaa aataatatta aattattaaa
    28501 tctttcaggt atacaaacag ggatatatag gctaggatta ttttccatgc tgatatatct
    28561 tttttttatg aaaaagtaca ggaaataata tcaaagttac ttaaatacag taggtctaat
    28621 tttctaggga gggttatata tgaatgaatt gaaaagagta tctttgtaca atatacacaa
    28681 agagcttgga gcaaaacttg ttgaatttgc tggttgggaa atgcctttag aatatgaggg
    28741 tataaataaa gagcatgaga aggtcagaaa aagtgcaggt atatttgatg tatctcatat
    28801 gggagaggta cagataaagg gagcagagtc agaaaaattt atacaaaacc tagttacaaa
    28861 tgatataagc acattaaaga taaatgacat tatatataca cctatgtgtt atgaaaatgg
    28921 tggagtagtt gatgatttat taatatataa atttggtgaa gaagattatt tattagtaat
    28981 aaatgcaggt aatatagata aagatgtagc atggataata aaacaaagtg aaggatacaa
    29041 tgtagatata aaaaatatat ctagtgaagt gagtcaatta gcaattcaag gacctaaagc
    29101 agaagaaatt cttcaaaaaa tcacagatat agacttaaac tcaataaaat tttataagtc
    29161 aattccatca acaaaagtat gtggttgtcc ttgtttagtt tcaagaacag gatatactgg
    29221 ggaagatggg tttgagatat actgtaaaaa taaatatgta gaaataattt ggaatgaagt
    29281 tctaaaggtt ggaggagaag atatatgtcc agctggtcta ggttgtaggg atactttgag
    29341 atttgaagca gctctacctc tatatggaca tgaaataaat gaacacatat ctccaattga
    29401 aggtggttta tctatctttg tgaaaacaaa taaagaatca tttattggca aatcaatttt
    29461 atcaaaagaa aaagaaagtg gagctaaaag aaagttagta ggatttgaaa tgcaaggtaa
    29521 aggaatgcct agaaatggat atgatataag gattggtgat aaaacagttg gatttgtaac
    29581 aacaggatgt gcatctccaa cgacaggaaa aattttaggt atgggaatta tagattctga
    29641 atatgcaaaa gtaggaaatg aaataggtat agcaatcaga aaaaaagttg ttcctgctgt
    29701 gatagt
//
LOCUS       DAGSRM010000038        28821 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.038, whole genome shotgun sequence.
ACCESSION   DAGSRM010000038 DAGSRM010000000
VERSION     DAGSRM010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28821
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.038"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            669..2903
                     /locus_tag="MJE33_001928"
     CDS             669..2903
                     /locus_tag="MJE33_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBY9647863.1"
                     /translation="MQNHNYIKMLNKKVEVLSKELDSRNFKLKTLNEIDFFSKIPNRE
                     FMYQHIDDCISKNIHIGIMLLNIDNFKLFNDIYGYLVGENLLITLTQNLLHFIPESKG
                     IVAYLKGEGFLIVLPNVSNKELNNMGATIIESARELKIKLPNEKEIYSNVTFSIGSTI
                     WNGEPNMTTLMLFNQVYSALKKAKSEGKNRYILFDFKDNSFYNKLENDKEFLSEISHV
                     LQTKQIEVFYQPLYNIKNNTIVGCEALARWRHPIKGLLTPDKFIPLFEQFGLITELDL
                     HLFEENCKNIRRWIDNNDVFVPICCNFSRLHFFNPNFPSMLKGITEKYSVSTSNFSIE
                     ITENILIENTNTIIQQLAELRSYGFSVSMDDFGSGYSSFGMLQNLPFDIIKIDRIFLS
                     HNLNDFKNTTILYSIINIARALGMLTVCEGVETEQHVEFIKSINCDIAQGYYYAKPME
                     CKEFEKLLSKENNSEKMFIKYSDIGKSLIEKIFDGFFIMQDMKKLRANVTEDIQWFDF
                     YNKTEITSFPKVIEHFEKHIKGKKFSILYKSITPHNTESDSLLISGEAVLSDEASEHQ
                     KYSNFYFSANCIISENKLLLAKLHLDLIKTAGYINEFESYSIPKMTNTHLDESNILSQ
                     FYSMLPVGIIRYDLFGDMIITYMNEEMFDIIGYTKEQFFGEMGGNLRAIVHPDDLDFV
                     YKKSLDMIDTNKIEPFLYRFIKRDGQIVTVMYTQCNLSAIDGRSIAQGMYVDVNKIKN
                     LTKI"
     gene            2934..3767
                     /locus_tag="MJE33_001929"
     CDS             2934..3767
                     /locus_tag="MJE33_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBY9647864.1"
                     /translation="MFKEIFLRTFPGFLIIRDSNYRIIFINDNLKNLIKSYMQDNPLG
                     MTNIEIAKKLPDNIAKFFTDSHNIQLDWEKNYPYDKISNWILEFKKDTTSYWNVLEYK
                     VDVDEKTYIITMANDITKLYEENKRNLHYSITDPLTGAYNRKYLNDRFDIFIGDYIVL
                     IDLDNFKMINDYEGHNVGDKILCDFVSLLNKELINSTSIIRLGGDEFIVIFSSDVDKN
                     YVYSQLEALRENFLKVFSKYKYLSFSYGVDTVKRNLKLTIVELDKKMYKNKKKFDKND
                     Y"
     gene            4007..5740
                     /locus_tag="MJE33_001930"
     CDS             4007..5740
                     /locus_tag="MJE33_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBY9647865.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDIDEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQDEIPSCDINNDIFETVSEDENRCMVLGRYTVRTKENTQMIL
                     EVNQRCTFEIIEDRGKLLVKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDNTYSFFYVNEGLPKILGYTYDEFMEMSGGSAVGAVYPPD
                     LPKALEDCERCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDIENDTLIKYQRVEIDKKIELENFETKN
                     YSKLLESGKIIHLDDIGKLLEVLHGNLRETIEIRQVNSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIEEYLCSSDSKNND
                     ALLVIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTHDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSTYE"
     repeat_region   6132..6757
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=6201..6229
                     /rpt_unit_seq="atttacataccacttagttaatataaaac"
     gene            complement(7287..7391)
                     /locus_tag="MJE33_001931"
     CDS             complement(7287..7391)
                     /locus_tag="MJE33_001931"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBY9647866.1"
                     /translation="MDDYIYYYNNFRYQNRLNSMSPFEFRKYLSTERA"
     gene            8016..8261
                     /locus_tag="MJE33_001932"
     CDS             8016..8261
                     /locus_tag="MJE33_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647867.1"
                     /translation="MNQKKIKISIKMVIIGIFAVIIAFVLSRLFVEYIDELLRSNWQY
                     QLFESESSYIVFANCIMFSNILLEIFLIYICRKFRKI"
     gene            9256..9905
                     /locus_tag="MJE33_001933"
                     /pseudo
     CDS             9256..9905
                     /locus_tag="MJE33_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429361.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase family
                     protein"
     gene            10446..11801
                     /locus_tag="MJE33_001934"
     CDS             10446..11801
                     /locus_tag="MJE33_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBY9647868.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISALTNIILDYLFVIEFGWGVKGAGIATGLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKTLRKIVFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATIIASVAVFAICM
                     AFSGPIVSLFIHPEETMLFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVLSLISMILIFGGQGIWMTTIVSEFICLILSLVFLKKNFSTLDSNLNKV
                     A"
     gene            complement(11919..12587)
                     /locus_tag="MJE33_001935"
     CDS             complement(11919..12587)
                     /locus_tag="MJE33_001935"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HBY9647869.1"
                     /translation="MKEVLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTIS
                     EEHGKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFERALIKAKNMGATICAY
                     GDIDIESHRKWDTDRCEAVGMKVDLPLWQESREDLVYEFIDSGFCSVVTKVNLKHLGE
                     EFLGKKLTRELVEKIKNAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLIKDGYGY
                     LEIK"
     gene            complement(12654..13082)
                     /locus_tag="MJE33_001936"
     CDS             complement(12654..13082)
                     /locus_tag="MJE33_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBY9647870.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGVFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVILLILGTFFIVN"
     gene            13189..13782
                     /locus_tag="MJE33_001937"
     CDS             13189..13782
                     /locus_tag="MJE33_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBY9647871.1"
                     /translation="MNFCYNCSIDFGGDFMYTNFNIDFNNFNKKENAIKFMLMGVLLI
                     ILGLLCLTFKTLGIKLISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWVL
                     IIACILLFVFPQKIQSIFSLFLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGIC
                     LILSPLFLSRFIVSIMSFFIILFGLVLFFSGNTARKY"
     gene            13901..14836
                     /locus_tag="MJE33_001938"
     CDS             13901..14836
                     /locus_tag="MJE33_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBY9647872.1"
                     /translation="MDKFKYAFDNKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SLGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVIGLSISTRPDCLPDDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMMETAKAVGKMGVD
                     GIKIHLLHVIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNQIDDTLKARNSYQGCKFV"
     gene            complement(15108..16052)
                     /locus_tag="MJE33_001939"
     CDS             complement(15108..16052)
                     /locus_tag="MJE33_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBY9647873.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLTSEQVAFTRLF
                     FGFIVLGVYSSIRTPQILKISKKGIIYSVIIGIICQAMFNLCYFKAIDIAGVSIAAVL
                     LYTSPLFLAIFSKICYKENITRSKLFSLILCFIGAIMAVTGGRLDFQGLNAFGLLLGV
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSRPWEILNYAKDLDVLSC
                     MLMLGIVPAALAYIFYAAGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFYKSNKLKQIEKTESI"
     gene            16370..17800
                     /locus_tag="MJE33_001940"
     CDS             16370..17800
                     /locus_tag="MJE33_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY9647874.1"
                     /translation="MNLDLEFYQKILEASHDEICVSDDKGIIIYCNKAFEENYGLKKE
                     DILGKNVSFLEDSGYSTKSPIPIVLKTKTKFSLEQDTQTGKKLIITATPIFDENGNLE
                     FTVENCRDITELNNIKNKLEDTKKQVKKYKSEVETLYRTALRIEDTVIMDGIVMRPII
                     NTVNHVSKTDVSVLLLGESGTGKSSLARYIHHNSNRSNGPFITINCATISPQLLESEL
                     FGYTSGAFTGASTKGKVGLVELANGGTLFLDEIGDIPQNLQAKFLQLIQDRTFTPVGS
                     LKNKKVDIRIISATNVDLVSKVKEKKFREDLYYRLNVIEIKLPPLRERRDNLVEIIKY
                     YFNRYSSDFNLNKTISKEAMDAIANYRFPGNIRELQNIIQKILLTCTDNHITIDDLPN
                     ILTKNIHITNNGNKTHISQINKVIISDSKSTNYKNKNFDTLIKEYEKNIILDAYEKFG
                     SSYKVAKHLEISQSKANRLIRKYTNT"
     gene            18000..18584
                     /locus_tag="MJE33_001941"
     CDS             18000..18584
                     /locus_tag="MJE33_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HBY9647875.1"
                     /translation="MLMNTHFIMAKSILDNIDENKTFFISEKNFIYGNIKPDAFSKYK
                     LKKHYLDESFNMIVSKINYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSE
                     RWEFKHSLNKHVTYERELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKKTDYMN
                     DLKFSAYTCNSVINYILDSILSNTIKSYHIANCG"
     gene            complement(18688..19467)
                     /locus_tag="MJE33_001942"
     CDS             complement(18688..19467)
                     /locus_tag="MJE33_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBY9647876.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDIVAIEEIEKKGVQFNVATGRI
                     FKQAHNIIKDSLDMNGYYVCENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKAREDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(19525..20781)
                     /locus_tag="MJE33_001943"
     CDS             complement(19525..20781)
                     /locus_tag="MJE33_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBY9647877.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFDESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILKKDGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEREAFKLIKPVEEKIKSRVGKYVYTEGDISISEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVTYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAIEMAEGVAKGLKANIGISTTGVAGPGGGTKEKPVGLVYTAIYINGKTIVKKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            21039..21938
                     /locus_tag="MJE33_001944"
     CDS             21039..21938
                     /locus_tag="MJE33_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBY9647878.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVEEAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            21962..23410
                     /gene="ddlR"
                     /locus_tag="MJE33_001945"
     CDS             21962..23410
                     /gene="ddlR"
                     /locus_tag="MJE33_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DdlR"
                     /protein_id="HBY9647879.1"
                     /translation="MIFSNLKLNDNEPIYIQLKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSEGLIYSISGKGTFVKSKNKSSNTTWSLNWDCLENTYSKK
                     ANELDIIKSEIPWSSDLISFKSISPDGDLFDIEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLEYMTNKGADISNKDILITNGFTEGFDIIMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFNMLEDNLVKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIAIIEDGFNEELLYNSSHIFPICSLDNMNNGVVYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYKCIKKYIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
     gene            23469..24275
                     /locus_tag="MJE33_001946"
     CDS             23469..24275
                     /locus_tag="MJE33_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBY9647880.1"
                     /translation="MNLILGDNMSKKSKRRKINKLKLTIVITVLFLALLSIYEILFSN
                     PNKKGNIKSQETMQNKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGK
                     SMPTDEPISFDSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEK
                     IKLIDDYDAVDEKSMSDNNTSSFCYRTIAGTNVVSNHGKGRAIDINPLQNPQVSGNDV
                     TPKVSTVYADRSSTKFGMIKKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            24301..26220
                     /locus_tag="MJE33_001947"
     CDS             24301..26220
                     /locus_tag="MJE33_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071223118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBY9647881.1"
                     /translation="MNLMTLENISKSYSEKKLLENISLGINEGEKIGLIGVNGTGKST
                     LLKIIAGVEESETGNITKTNGIRIEYLPQNPTYNENSTVLEQVFNGTSEELKLLGEYQ
                     EILEKLNSNFTEELNKKLLSVHEKIDALNLWDLESEAKSVLTKLGINDFNQKIAELSG
                     GQRKRVSLASALITPCEFLILDEPTNHLDNDTIDWLEEYLNSRKGSLLMITHDRYFLD
                     RVTNRIIELDKGRLFSYDGNYSIFLEKKMERLSLEASIEDKRQNLIRTELAWVRRGAQ
                     ARSTKQKARLQRFDELINRDSFKPDENLDISVASSRLGNKIIEIHNISKSFEENTVID
                     NLEYTLTRTDRIGIIGKNGMGKSTLINILNGEIEPDSGYISIGETVKIGCFSQDDSHM
                     NIDMRAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKLSGGERRRLHL
                     LKILMTAPNVLLLDEPTNDLDIETLKILEEYLDEFKGVVVTVSHDRYFLDRICNKIFA
                     YEGNGKIHIYTGNYSDYLIYREIQGIEFEESKRESVKKDDSTTPKKEKPKNDKKLKFS
                     YNEQREFENIDSDIEKLESKIATLDESTAKFSTDFTKLQEILDEKSKLEKELEYKYER
                     WEYLNNLADEIANNK"
     gene            complement(26462..26809)
                     /locus_tag="MJE33_001948"
     CDS             complement(26462..26809)
                     /locus_tag="MJE33_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HBY9647882.1"
                     /translation="MSKSNDVLDIVFKITDGLEYEVAENRVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(26796..28724)
                     /locus_tag="MJE33_001949"
     CDS             complement(26796..28724)
                     /locus_tag="MJE33_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HBY9647883.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFLEGLAVGVGGVLFGIGIAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSAGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGSK
                     NEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGSL
                     PWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEKE
                     KEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKVPSGFAATNSMAIALLVVLV
                     VLTALPIVLSKVKNNEQE"
BASE COUNT        10981 a         3946 c         3742 g        10152 t
ORIGIN      
        1 aacatcctaa taattaatta ttaaaatatt tttatattgt aatattaggt atagtattta
       61 taacttcaac ttttttataa atatatttta gttttatata gttttatgtt tacagctatt
      121 atatccatct ctatgtaata tttttcatat taaaactgat ttctatagat taatatttta
      181 taaatttagt aaatgaatat ttattatagt ctttaacaaa aaatacaaga ataaacttaa
      241 ttttaataac taaaattaat tatgcaaaat tattaatatg gtattaaatt atgtataaaa
      301 agttaattat gaataaaata attatgcctt tgaaaaatca ttaaacttaa aattaattat
      361 aaaaaaactc taccttacta actatacagc tcacaatcta tctatttatg aaaaaatatg
      421 atataattgc attcaaaaag gaaatctttc aatatactag aatttaagca ttaactaatt
      481 ttatctaata ttattactaa actaaaaaat atttgtattt gtagcattgg agcaagtaac
      541 ttaatgtata ttcattttaa atacttattt aaaatttagc atttaaatta tttacaaatt
      601 aacatttaaa tacttctagc ataaatacag ttgtcattaa aatttaaagt atcttatgga
      661 ggaataccat gcaaaatcat aattatataa aaatgttaaa taaaaaggta gaagtattat
      721 ctaaggaatt agatagtaga aattttaaat taaaaacctt aaatgaaata gatttttttt
      781 ctaaaatacc aaacagagaa tttatgtacc aacatattga tgactgtatt tcgaaaaata
      841 tacatatagg aatcatgtta ttaaatatcg ataactttaa attatttaat gatatatatg
      901 gatacctagt cggagaaaat ttattaatta ctctaactca aaacctattg cactttattc
      961 cagaatcaaa gggaatagtc gcttatttaa aaggagaagg ttttttaatt gtacttccaa
     1021 atgtatcaaa taaagagtta aataatatgg gtgcaactat tatagagagc gcccgagaat
     1081 taaaaataaa acttcctaat gaaaaagaaa tatactccaa tgttacattt agtatcggca
     1141 gcactatctg gaatggtgaa ccaaacatga ctactttgat gcttttcaac caagtatata
     1201 gtgctttaaa aaaggctaaa agtgaaggta aaaatagata cattctattt gattttaaag
     1261 ataatagttt ttacaataaa ttagaaaatg ataaggagtt cttatcagaa atatcacatg
     1321 ttctacaaac taaacaaatt gaagtatttt accaaccact ttataatata aaaaataata
     1381 ctatagttgg atgtgaggca cttgcaagat ggagacaccc tattaaagga cttcttactc
     1441 cagataaatt tattccttta tttgaacagt ttggtctgat aacggaatta gatttgcatt
     1501 tatttgaaga gaactgtaaa aatataagaa gatggattga taacaatgat gtttttgttc
     1561 caatttgttg taacttttct cgtcttcatt tttttaaccc taattttcca agtatgctaa
     1621 aaggtattac tgaaaagtac agcgtctcta catcaaattt tagcattgaa ataacagaaa
     1681 atatattaat agaaaataca aatacaatta ttcaacaact agctgaactt cgttcttatg
     1741 gtttttctgt atcaatggac gattttggtt ctggatattc atcttttggt atgttgcaaa
     1801 atctaccttt tgatataatc aaaattgata ggatattttt atctcataat ttaaatgatt
     1861 ttaaaaatac aaccattttg tactctataa taaacattgc aagagctctt gggatgctta
     1921 cagtatgtga aggtgttgaa acagaacaac atgtagaatt tattaaaagt ataaattgtg
     1981 atatcgccca aggttattat tatgcaaaac ccatggagtg caaagaattt gaaaaattac
     2041 tatctaaaga gaataattca gagaaaatgt ttataaaata ttctgatata ggaaaatctt
     2101 taatagagaa gatatttgat ggatttttta ttatgcaaga tatgaaaaaa ctaagagcta
     2161 atgtcactga agacattcaa tggtttgatt tttacaacaa aactgaaatt acatcttttc
     2221 ctaaagtaat agagcatttt gaaaaacata ttaaaggtaa aaaattttct attctttaca
     2281 aaagtataac cccacataat actgaatcag attcattatt aattagtgga gaagccgttt
     2341 tatcagatga agcttctgaa catcaaaaat atagcaattt ctactttagt gcaaattgta
     2401 taatttcaga aaataaatta ctcttagcaa aattacattt ggatttaatt aaaactgctg
     2461 gctatataaa tgaatttgaa tcatattcaa tacctaaaat gacaaataca catcttgatg
     2521 aaagtaatat tttatcacaa ttttattcaa tgttgcctgt aggtataatt cgctacgatt
     2581 tatttggtga tatgattatt acttatatga atgaagaaat gtttgatatt ataggttata
     2641 caaaagaaca gttttttggt gagatgggcg gaaatttaag agcaattgtt catccagatg
     2701 atttagactt tgtttataaa aaaagtttgg atatgattga cacaaataaa attgaaccat
     2761 ttttatatag attcataaaa cgagatggtc aaattgtcac agttatgtat acacaatgta
     2821 acctttctgc aattgatgga aggtcgattg cccagggtat gtatgtagat gtaaataaaa
     2881 taaaaaattt aacaaaaatc tagtacaaag tattttattt tggagaaatt aatatgttta
     2941 aagaaatttt tttaagaact tttccaggat ttctaattat aagagattct aactacagaa
     3001 ttatatttat aaatgataat ttaaaaaatt taattaaaag ttatatgcaa gataatccac
     3061 ttggaatgac taatatagaa atagctaaga aactccctga taatattgct aaatttttta
     3121 cggattctca caatattcaa ttagattggg aaaaaaatta tccttatgat aaaatttcaa
     3181 attggatttt agagtttaaa aaagatacta caagctattg gaatgtttta gaatataaag
     3241 tagatgttga tgaaaaaacc tacataatta ctatggccaa tgatataaca aaactctatg
     3301 aagaaaataa gagaaatctt cactactcaa ttactgaccc attaactgga gcatataata
     3361 gaaaatattt aaatgataga tttgacatat ttataggaga ttatattgta ctaattgact
     3421 tagataattt caaaatgata aatgactatg aaggtcataa tgtaggagac aagattttat
     3481 gtgattttgt ttcactttta aataaagaat taataaattc tacttctatt ataagattag
     3541 gaggagatga atttattgtt atattctcta gtgatgttga caaaaattat gtttattcac
     3601 aactagaagc cttaagagaa aattttttaa aagtattctc taagtataag tatctttcat
     3661 ttagttatgg tgttgataca gtaaaaagaa atttaaaatt gacaatagtt gaattggata
     3721 aaaaaatgta taaaaacaag aaaaaatttg ataaaaatga ttattaatat tgttatttta
     3781 aaataaatta aaattatctt tcttttttta aacttgtaca ttagtttata aatatgtatc
     3841 tatacaagat attgacatta agtactgaag catatattgt ttcagttctt ttttgttaaa
     3901 attaacttat ttaacaaaga tatcatagtt taacaaagct tttaatttaa atatattgat
     3961 gtataatata attaaaatat aatagcaaaa agaaaggagg aatatcatga gcatactcct
     4021 aaaaaaagca cccaaattag caaaacatat tataacaagt ttttatataa atcgggatat
     4081 tgatgaagta ttaaaatacc tttgtgaaaa tgttacatgg atcggaccag gagaacagga
     4141 atttctcact tcttttaatg aaataaaaaa ttatttttat gcaggtcaag atgaaattcc
     4201 ttcttgtgat ataaataatg atatatttga aactgtaagt gaagatgaga atagatgtat
     4261 ggtattagga agatatactg ttcgaactaa agaaaatact caaatgattt tagaggtaaa
     4321 tcaacgttgt acatttgaga taattgagga tagaggaaaa ttattagtaa agcacatgca
     4381 catatcaaac ccttatggtg aaatgcaact tgatgaatac tttcccacta aaattggaac
     4441 tcaaagttat gattatttgc aaagattact aaaggaaaaa acagaagtta ttgaaatgat
     4501 tacaaataat atcaatggtg gcttaaaagg tagcaatgat gataatacat actcattttt
     4561 ctatgtcaat gaaggattgc ccaaaatatt aggatacaca tatgatgagt tcatggaaat
     4621 gagtggtggt tcagcagtgg gtgctgtata tccaccagac ttacctaaag cactagaaga
     4681 ttgtgaacga tgttttgcaa aaggacctac atattcatct gaatatagaa tccgaaaaaa
     4741 agatggaaca ttaatgtggg tattagattc tggaatgaaa tcattaaata gtgatggcat
     4801 agtaaaaatt aatagtatta ttactgacat aacccaacta aaaaacatag aatctgaact
     4861 aaaattagaa agagagcgat atcgtattgc tttgcaaaat attacagata tcatgtttga
     4921 gtatgatatt gaaaacgata cccttataaa atatcaacgt gtagaaattg acaaaaaaat
     4981 tgaattagaa aattttgaaa caaaaaatta ctccaaacta ttagaatctg gtaaaattat
     5041 tcaccttgat gatattggaa aactactaga agtacttcat ggaaatcttc gtgaaacaat
     5101 tgagataaga caagtaaatt cattaactaa aaatgaatgg agatggattc gtgttcaatg
     5161 ttcagttata tatgatagtg accataaccc tatcaaaaca ataggcgttt taaaagacat
     5221 aactgaagat aaatctaaat tagaaagctt aattaatcag gctcaacgtg acccactaac
     5281 acaattatat aatcaaagag tatcacaaaa tcttattgaa gagtatctat gtagttctga
     5341 ttccaaaaat aatgatgctc tattagttat tgatattgat gattttaaaa ctgtaaatga
     5401 tacttttggg catttagagg gaaatgaagt tttagttgct gtatcaaaaa tactattaca
     5461 taacacacat gataaagaca tagttgctcg aattggtgga gatgaattta caatatttat
     5521 aaaatcatta acaaaggatt taataataaa aattactaat gatattttaa atgatgcaag
     5581 taaaataaaa gtaaaggaca atcataaaat aacattaagt attggtattg ccttcactga
     5641 tgattcaact aaattatata aagatttatt ttctaaagct gataaagctc tttatctatc
     5701 aaaagcagat ggcaaaaatt gttatagtac atacgaataa taaagctatt tatacgacca
     5761 agaatgaacc tttaaattta atttcatagt ctaacaatca aagagttgaa gctaatctta
     5821 tagctagtca actagttcct agaaaaaatt taaacaatat atttattgat actgctcatt
     5881 tttctaaaga gcaaatttat ataaaaaatt aaacttttat gtacatacag atactaatga
     5941 taaacagtta tcattagtat cttctatctt aaaacaaaat gataatttat ctatataaaa
     6001 aagtgctttg ttgacagagt tcactttttt atataggtac agaatttaag tataaataca
     6061 tgttttattc tatacatttt agttaatgta aaatttcttg attgtactct ctaacttttc
     6121 aacattatac tatttatata ccacttagtt aatataaacc ccttagctgg ttctagtgct
     6181 ttttctatga ttttagtcat atttacatac cacttagtta atataaaacc aatttagatg
     6241 atgtatatgt gcaaaaggaa gaaataattt acataccact tagttaatat aaaacgctac
     6301 taaacttgat atacatgacc attataataa taaatttaca taccacttag ttaatataaa
     6361 acttccataa aaacatcccc tttttattct tcttccttat atttacatac cacttagtta
     6421 atataaaaca gtcttttttt aatgcagaga tgcaagaagt tttataattt acataccact
     6481 tacttaatat aaaactatca gtatttaagt aataatttaa gtaatatagg atttacatac
     6541 cacttagtta atataaaacg gtcactacaa cttatacttc cagtctaacg atttgaattt
     6601 acataccact tagttaatat aaaacatatt ttggatttat taatataaac ataattaaaa
     6661 aatttacata ccacttagtt aatataaaac tataaagatt tagaaaaaga aaaagttaaa
     6721 attctagaat ttacatacca cttagttaat ataaaacccc aaaataaact gggcattttc
     6781 aatacttaca catatgcaac tctcttaaat ttgcagtgaa ccatgagtag tgcaaatgat
     6841 aaagcttatc atataccctc aacaccttat atttcaagcg ttaagctcta ctttgtaaca
     6901 aatatcgctc actgcaaaac tatttacatt tttattatat cataaatata ttatttttga
     6961 atatctatac caatttgtgg tataataaaa gcaagaagaa ctacaatcta tttagacgta
     7021 gagtgaagtt caaatactaa tgtttattct ttttgaactt gatttttagt tcaaaactaa
     7081 agtcactcct gcctgtgtga actatacccg ctgttttata cagttaaaac atgtaaaaat
     7141 agttataaag tactagtgta actcattagt actcttttta ttatattcat ataattaata
     7201 tctatatttt aaagtttact atctaaaaaa gagcacgtca aataaaaccc tagatattta
     7261 atttttttag agatacttaa aataaattat gctctctctg tacttaaata tttcctaaat
     7321 tcaaatggag acatagaatt taatctattt tgatatctaa aattattata gtagtatata
     7381 taatcatcta ttgctttaac taattattca tatgtatcaa atttccttaa atagtacatt
     7441 tctaccttta aaattcccca aaatccttcc ataggagcat tgtcaaggca acgaccaact
     7501 cttgtataat atatgtatat ctaacaccat taagaaatat atattttata atatatattt
     7561 cttcatttaa tgcacttaga aaagcaaaga aaatacagat tagtacactt tttagagaaa
     7621 aataaaatta atacatctaa aatattaaaa aatactttag ataatctaaa gacacatctc
     7681 aaagttttta actaaattat aaaactaatt acaaatgcta tgtaacgata ataaataaaa
     7741 aatttacttc gcttaagcga cgtgtcgttg aaatatttca tgttgccaac tggttcgtaa
     7801 gttgataaga aaagaacact aattatttag agttcttttc ttagctacaa gtggattata
     7861 atacttttaa atctgtgaga aaatcatata taataatgat atgaacaagt aaagaaaata
     7921 caaaaatgac aaaattataa ataaaatatt ataaaatcat aaggtcataa ttgttatatt
     7981 gtagaaacaa agtaaagaga gagggtggag aataaatgaa tcaaaaaaaa ataaagatta
     8041 gtattaaaat ggtaattata ggtatatttg ctgtaattat tgcttttgta ttatccagat
     8101 tattcgttga gtatattgat gaattattaa ggtctaattg gcaatatcag ctatttgaaa
     8161 gtgagagttc atatatagta tttgccaatt gtattatgtt ctcgaatata ttattagaaa
     8221 tatttttaat atatatttgt agaaaattta gaaaaatcta atatggtcaa cgtatttcga
     8281 atcgaatact tcatgattat ttagtctatt gtgctagtta aagtttaatt cccatataaa
     8341 gtaaattgat tttagtaaaa caaatcaccc ctaagggtta gtttatggat aagtcatcca
     8401 aatattaact ctttaatttt tgctatagat tcatctttgc tcataacttt acttataaaa
     8461 aatgaaatat tataagctag aacttttatt ctaattctag tcataaatcc tagcatagag
     8521 ttactttttt tatattataa ataaaactat acaacaaaga taatatttag taaaaataac
     8581 tttcatttta actaaactta aaattcaatt actctctaaa ttgacgttta ggaattagtt
     8641 caaaataaaa aagagtgtat atattgaaat tactacattt caatatatac actcttttta
     8701 aatttctcag ttttccatgg aactttactt tatttatttc tgtattagtt gtatttttta
     8761 tgcatttttc tttaaaatca tattttctga ttcaactctt atattataaa atttctttac
     8821 taaattattc tctttttcta tatcttcata tatatttttt aatcgccttt tacctaatct
     8881 tttatctaac attactaatg atttaactaa tatattatat gattttatcg catcttctat
     8941 tgagatattt aaacatatat ttactgcttc ataaaaatca aattcattaa aaaatccatt
     9001 tttatttaca atcttatatc cctttgcatc tctttctaaa tactcttcct aaatactctt
     9061 caaaactttc atcatctaat ctaaatccta tatcactatg aaaaataggt tttgcatttg
     9121 gatatagttc tattgtttta tcaaaagttt cgaataccaa gttattatta ttcgattttc
     9181 caacagtcca ctataaacaa taataagttt gttgctccaa ataatgtaac acttgtagaa
     9241 agatgttaca gatatataaa agatagagaa tgtagttttt ctcctaatac tattagaaat
     9301 agaaaaaata tgattaaaaa tcaaattgaa gttttcttta aagatatgaa attaatagat
     9361 ataactcctt ctattttaca gtcttatata aataacatat acaatgagca catgttaaat
     9421 tcaacaaaaa accaagtcga ttttataaaa tcagtattga aagaatctta tagattaaaa
     9481 gaaatatcag aaaatatttg tgattttgtt acaaccccaa tcaaaaaaaa tagttcaact
     9541 tctaagcttt atacaaaaca aaaagctcaa ttattattag aaaaatcaaa aaatattcct
     9601 ataggtatac caattttttt gatgctaact cttggattac gttttggtga agctgtatca
     9661 cttatatggt cagatgtaga ccttgataaa aaatctcaca tgtaaatcaa acattagttt
     9721 atgtatacaa taaaataatc ttcaaaggcc ctaaaacccc tagattcaaa agaagattat
     9781 ttgttcctga tgaacttata agtttgttta aaggggaaaa attaaaacaa aataagttaa
     9841 aattacaggg tatattgaaa aatgaatttg atttgatatg cttaaataaa agattcaacc
     9901 cgtgagttca accaactttt gttgttcatt ttaaaaaact attaagagat aataatccta
     9961 gagatataaa agtacatgaa ttaatacatg caaatgcgac tttgatatta ctttcaggta
    10021 ctaacataaa agctatttat aaaatattag tacatgcaga tataaaaata gctatgaata
    10081 tatattccat attctcactt attagatgaa atgaataaag aaacatctga aaatataagc
    10141 aaaatactat tcaaataatg ttcagtttta tgtcaattaa atgtcactca aagtgctttt
    10201 tttccatttg tcagttaaat gtcacttaaa gtaccttttt cctatttgtc agttaaatgt
    10261 cagtaatata taaataagat atatttataa gacccttaaa atcactatta tttaataaaa
    10321 tttataatta tatactattt gctgttgaca ttggtattat accatagttt atcatttata
    10381 ttagaataaa attattaatt aatagtttcg taattttata atagatttac agggagtgat
    10441 aataaatgga tacaaaaata tctataaacg aaaacatgag tctaggtaaa agatttttta
    10501 aatacctagc accatctgta gttgcaatgt gggttttctc actatataca atggttgatg
    10561 gaatatttgt aagtaaagga gttggagaat tagctttagc tgctgtcaat atatcaatgc
    10621 cttttattaa ttttatattt gcagtttctc tattattttc aactggtgca tctaccataa
    10681 ttgcaatcta tctggggaaa aaagatatta aatctgctaa cgaagtcttt tcatttaact
    10741 tagtatcaat aattatatta tcaataatta tattagctat aacttttttt aatttagata
    10801 gattagcact atttttagga gctacagaat ctacaattgg tatggttaaa gattatttag
    10861 gaataataat attttttaac ggattcttta tagtgtctta ttctttagaa gttataataa
    10921 aaactgatgg ctttccaata ttagctactg ttggtgtaat aatatctgct cttacaaaca
    10981 tcatacttga ttacttgttt gtaattgaat ttggatgggg tgtaaaaggt gctggtattg
    11041 caactggttt gtctcaagtc ttttctacta ttttcttctt aatacatttt ttaagaaaaa
    11101 actctacttt gaatttttca aaatttagaa ttgattttaa gacacttaga aaaattgtat
    11161 ttataggttt tcctgattca actactgaat taagttgtgg tatagttgtt ttacttttta
    11221 acttaagtct tactaaatat attggagaaa atgctcttat ttattatagt gtcataaatt
    11281 atataaatac attagtactt atgactatga tgggtataac tcaaggtatg caaccactta
    11341 ctagctttta ttatggtgca ggaaatatag ataatgtaaa aaaattatta aaaatgggga
    11401 taaaagccac aataatagca tcagttgctg tatttgcaat ttgtatggct ttttcaggac
    11461 caatagtctc tttatttata catccagaag aaacaatgct attcaatgaa ggagttcgtg
    11521 tatttaaaat attctctata tctttcttat tggtaggaat taatgtaata atttctggat
    11581 tctttgtatc agtagaaaaa ccatcaattt ctactgttat atctcttggt agagggcttg
    11641 taatagttgt tttaagtcta atttcaatga tactaatatt tggtggacaa ggaatatgga
    11701 tgactactat tgtttctgag tttatatgtc ttattttatc attagttttt ctaaaaaaga
    11761 atttttctac attagactct aatctaaata aggttgctta gatgataaga gtgacaatat
    11821 atgaaaatat ctaaataaaa aatacctatt atacaatctt gcaaatatta aacctttgca
    11881 aaaattagga ttatataata ggtattttat ttatgtaatt attttatttc taaatatcca
    11941 tagccatctt ttattaaagt tccttttact tcatattcta caggtttttt aaatattgga
    12001 ccatctacga caaatgtatg atactctcca tgttctccac atgggtctgc accagcattt
    12061 tttatcttct ccacaagttc tctagttaat ttttttccta aaaattcctc tcctaagtgc
    12121 tttaagttta cttttgttac aacactacaa aatccactgt ctataaattc atatactaaa
    12181 tcctctctac tctcttgcca taatggtaaa tctactttca taccaactgc ttcacatcta
    12241 tcagtatccc attttctatg actttctata tctatatctc catatgcaca tatcgttgct
    12301 cccatgtttt tagcttttat taaagccctc tcaaagctct cttcatatcc ttctacgcca
    12361 cactcagcaa ctaataaagg taatcctatg ttgctactta cttgcttaag catattatat
    12421 tctaaattat gtgtccatga ctttccatgt tcttcactta tagttgtcaa gagtgccaca
    12481 ggcttatatc cttttttcaa cattcgattt aatgctaata tactatcctt accaccacta
    12541 aatgacatta caaatttttc atcagccaat tttaacacct ctttcatcta actttgtttt
    12601 attaacataa ttataccaaa tttttgagtt gcatagcaaa ttatattaaa ttgttaattc
    12661 actataaaga atgtccctaa tattaaaagt ataaccccta tgtatttgta taaatgcatt
    12721 gtttcttgtc ccaaaattac gctatcaata aaaaatccaa ctaaaacttg ggatacaaga
    12781 gttaatataa gtgtatttga tactcctaaa tttggaattg cctttactgt aaaatatatt
    12841 attagacctc caaatactcc tcctaataac aacttataat taaaaccatt tatagatact
    12901 agacacttta agtcacctgt aactattata cttgccaaaa aaaatattgc tcctacagtt
    12961 aagcttataa aggtggccac taatgctgtt gtactttttc ctagctctcc atttataaaa
    13021 gcttcaagag ctgtagaacc acctgctaat aatgcaaata ttattgccca taattttgtc
    13081 atggacaaac ctccttctat ataattctat taatagaagt ttatgataca acctattaat
    13141 taattactaa gtatagttta atttttatac actaggcttt atttggatat gaatttctgt
    13201 tataattgtt ctatcgattt tggaggtgat tttatgtaca caaattttaa tatcgatttt
    13261 aataacttta ataaaaaaga aaatgctatt aaatttatgt taatgggagt attactcatt
    13321 atattaggat tattatgcct tacatttaag acattaggta taaaattaat ttcttggact
    13381 tttggtattg cacttttatt ctttgcatat ttaaatttaa aaaatattaa tgagttaaaa
    13441 agatatgcta ctaaagaaga aattaaaccc tctattaata tccaatgggt tctaataatt
    13501 gcatgtatat tactttttgt atttcctcaa aaaatacaaa gcatattttc attattttta
    13561 ggattttatt taatctttaa tcaactagta gcacttgtaa atagtaagaa taatccttat
    13621 tctaaattta caacttggaa tatagttaag attctatttg gtatatgcct tatattatct
    13681 ccactttttt tatctagatt tatagtatcg ataatgtctt ttttcataat attatttgga
    13741 ctagttctat ttttctctgg aaatacagcg agaaaatatt aattattttc tcgctgtatt
    13801 tttatattac ttaaaataga atttgtttaa gttatctatt tgtggtaata tgttttttgt
    13861 acaagctcag cgtacaattt tattagaaat aggtgatttt ttggataaat ttaaatatgc
    13921 ttttgataat aaaagatatc atacttggaa ttactatctt agaaatactt ttggagaaaa
    13981 agtttttaag gtatctataa atgcaggatt tacttgccca aacattgatg gaagtcttgg
    14041 ttatggcgga tgtacctatt gtagcaaaga aggctcaggt gattttgctg gaaaccctaa
    14101 agataatctt atcagtcaat tttataatat aaaagaaatg atgttaaaaa aatggcctca
    14161 tgcaaagtat attggctact ttcaagccta tacaaatacg tatgctcctc ttgaagtttt
    14221 aaaagaaaaa tatgagacta tattagaact tgaagatgtc ataggtcttt caatatccac
    14281 aagacctgac tgtttgccag atgatgttgt agaatactta tctgaactaa ataaaagaac
    14341 caatttatgg gttgaacttg gacttcaaac aattcatgat tctacatcta aaattataaa
    14401 tagaggtcat gattataaaa catttgttga aggtgttgaa aaactaaagt ctaaaaatat
    14461 aaaggttgtc gtacacatta taaatggtct tcctggtgaa gattataata tgatgatgga
    14521 aacagcaaag gcagttggta aaatgggtgt agacggtata aaaatccatc tattgcatgt
    14581 tataaaagat actcctatgg aaaagatgtt acaaaatggt atgttaactc ttatggaaca
    14641 agatgaatat atcaatcttg tctgtgacca attagaaata cttcctgaaa ctatgataat
    14701 ccatagactc actggtgatg gtaagaggga tgaactagta ggtcctatat ggagcctaaa
    14761 aaaatgggaa attttaaatc aaattgatga tactttaaag gctagaaatt cttatcaggg
    14821 ctgtaagttt gtttaataca aaataataac tatagaaatg gttatataaa tatagtcctt
    14881 tctatagtta tttagtattt acaaaaacaa aatattaatt tgtacattta tacaatatat
    14941 aataaaaaat agatagtcta aactgtatct acttatattt tatttgtact gtccacttta
    15001 ttctataaaa tttataaata gttatttttt aataccaaat ataatttggt ataatttttg
    15061 ttggactcta aaatgtatac caataaatcc tttttaagta tataattcta aatactttca
    15121 gttttttcaa tctgctttaa cttgttggac ttataaaata ttcttatttc atcataagaa
    15181 agattaagtg ctactactgc agatattaac atcaacatca ctccaaataa cttccctaag
    15241 gaaaaacttt ctcctaatat agtgcaacca attattactg aaccaactaa ttctactgat
    15301 gccacaacac ctgcaactga caactctaca cctttagata tacctgctgc ataaaaaata
    15361 tatgcaagtg cagctggaac aatgcctaac attagcatac aagataaaac atctaaatcc
    15421 ttagcataat ttaagatttc ccaaggtctt gaagaaggaa tcataaatat agctccaaat
    15481 aagaaactgt atactaatat tgttgaactt gaaaattcct ttaaagcatt tttactaata
    15541 gttggcataa gtgcatatgc tattgctgat aataccccaa gtaaaagtcc aaaagcgttt
    15601 aaaccttgaa aatccaaacg tccaccagtc actgccataa ttgcaccaat aaaacaaaga
    15661 ataagtgaaa ataatttgct tctagtaata ttttctttat aacatatttt tgaaaatatt
    15721 gctaaaaaca gtggtgatgt atatagtaaa actgctgcaa ttgatacacc cgcaatgtca
    15781 attgctttaa aataacataa attaaacatt gcctgacata taatacctat tattactgaa
    15841 tatataattc cttttttgct tatctttaag atttgtggtg tccttataga tgaatatacg
    15901 cctaaaacta taaatccaaa aaacaatctt gtaaatgcta cttgttctga tgtcagtcca
    15961 tttcccatta aaaactttcc aaataatcca agtgtagccc ataataaccc tgctattgct
    16021 ataaatacat accctttact tttttctccc attttaaatt cccttctatt tttatttagt
    16081 caatttacac tcttcttttt aagcaaaaac tatgccatct taaatcaata tatatcgact
    16141 ctaaaaccct agtctttcca ataatactaa taaattttca atttttttat atttattttt
    16201 cacttacttt aagttgattt agacttattt taattgtaaa atgagttata tataacttat
    16261 ttaaagttaa aatagactga aacttatata taaatatcaa attttaagta tattaatcaa
    16321 ttaaacctat tttaatatat cattaaaaat ttaggaggaa aatataaaaa tgaatttaga
    16381 tttggaattt tatcaaaaaa tattagaagc ttcccatgat gaaatatgtg tttctgatga
    16441 taaaggaata ataatatatt gtaataaagc atttgaagaa aattatggtc taaaaaaaga
    16501 agatatttta ggaaaaaatg tttctttttt agaagactct ggatattcaa caaaaagccc
    16561 cataccaatt gtattaaaaa caaagactaa attttcactg gaacaagata ctcaaacagg
    16621 gaaaaagctt atcataactg ctacccctat atttgatgaa aatggaaact tagaatttac
    16681 tgttgaaaat tgtagagata taactgaact taataatata aaaaataaac ttgaagacac
    16741 aaaaaagcaa gtaaaaaaat ataaatctga agtagaaaca ctatacagaa ctgcacttag
    16801 aattgaggat actgtaatta tggacggaat agttatgaga ccaatcataa acacagtaaa
    16861 tcatgtttct aaaacggatg taagtgtact tttattgggt gaatctggaa caggaaaaag
    16921 ctcacttgct agatatatac accacaacag caatagatct aatggcccat ttataacaat
    16981 aaattgtgca actatatctc cacaacttct tgaatctgaa ctttttggat atacttcagg
    17041 tgcttttaca ggtgcaagta ctaaaggtaa agttgggctc gtagaattag ctaatggagg
    17101 aactcttttt ttagatgaaa taggagatat accacaaaat ctacaggcaa aatttttgca
    17161 attaatacag gatagaactt ttactccagt tgggagctta aaaaataaaa aggtcgatat
    17221 aagaattata tctgctacaa atgttgattt agtttcaaaa gtaaaagaaa aaaagtttag
    17281 agaagattta tactatagat taaatgttat agaaataaaa ttgcctcctc ttagagaaag
    17341 aagggataat ctagtggaaa ttataaaata ttattttaat aggtactcaa gtgacttcaa
    17401 tctaaataaa actatttcta aagaagctat ggatgctatt gcaaattata gattcccagg
    17461 caatataaga gaattacaaa atataattca aaaaatcttg cttacttgta cagataatca
    17521 tataacaata gatgacttac caaacattct tacaaaaaat atacatatta ccaataatgg
    17581 aaataaaaca catatatccc aaattaataa ggttattata tctgactcaa aatctactaa
    17641 ttataaaaat aaaaattttg acactttaat taaagagtat gaaaaaaata taattttgga
    17701 tgcttatgaa aaatttggta gttcttataa agtagccaaa caccttgaaa tctcacagtc
    17761 taaagccaat aggttaatta gaaagtatac taatacttaa ttttaaattt gataaataaa
    17821 aacttaacac tatttacata ttgtaaattt ggccatttac tgttattatg aatatggttg
    17881 aaattattta aataaatact gttgacttag tttcatatat ttaccattaa acttttatca
    17941 taaatatcat atcataataa tatatttaat aagtctaata aactatacga ggtgtaaata
    18001 tgttaatgaa tacccatttt attatggcaa aatcaatact tgataatata gatgaaaata
    18061 aaacattctt tataagcgaa aaaaatttta tatatggcaa tataaagcct gatgcttttt
    18121 ctaaatataa attaaaaaaa cattatttag atgagtcatt taatatgatt gtttctaaaa
    18181 taaactatct atgcagtctt aatatagata gtctttctaa atacttttca gtaagtagac
    18241 ttagtcagga actaggagtc atatgtcatt ttttatgtga ttttttctgt gtagctcaca
    18301 gtgaacgttg ggagtttaag catagcttaa ataaacatgt tacttatgaa agagaacttg
    18361 gagcatttgc aaaagatgtt gatttatcta aaattaaatg tggtactata aatagtagtt
    18421 ttgagaagtt ttttactaaa ctttatagcg attacaaaaa gaaaactgat tatatgaatg
    18481 atttaaaatt ttcagcttat acttgtaata gtgtcataaa ttatatactt gactctattt
    18541 tatctaatac aataaaatca tatcatatag caaattgcgg ttaacttaag aatttttaaa
    18601 cttatataaa cttataattg aatataatta aaatgactgc tctatagaat ataattttat
    18661 acttctctag gacagtcatt ttttatacta actatttatc tccattataa catctatggc
    18721 actttttaca ctatctacta cataatttgc actttgcttt acatcttccc ttgccttaga
    18781 cattgcaaaa ctatatttaa atccttctaa catgcaaata tcatttggag aatccccaat
    18841 agtagcaact tcatctggac ttacatttaa cgtttttatt acatgctcta tacttcctct
    18901 ttttgacaca cttttaggaa ctatatttaa agtatatgtt ccagaaaaat atatttctaa
    18961 gacttcacta aactcacttt ttagataaag ttctattctg gataactctt ccaaattttc
    19021 agaacatacg cctatatttc ctataagatt atcaaaacta cttctttttt caaagtctgg
    19081 gtcaactata aagtcactag aataatgtct aaaagcagtg gtatcactac ccacaattac
    19141 atcaccctta tatttaaaat aaagttgtgc atcacttgac tcaaatctat caatgacttt
    19201 ttttactaaa ttatcatcta tcgttctttt aaaaattaca ttataatctt tatcatatat
    19261 aaatgaacca ttttcacata catagtatcc attcatatct aaactatctt tgataatatt
    19321 atgtgcttgt ttaaaaattc tacctgtagc aacattaaac tgtactccct ttttttctat
    19381 ttcttcaatt gctactatat cttcttttgt tatggttcca ttttcatata gagttccatc
    19441 taaatcacaa aatatatgtt ttatcataaa ttaaaagcct ccaaaaacaa ttttatattt
    19501 tactaatact ttaatcaaaa taacttaaag cttttctaat tgtattctca actcattaag
    19561 caaatctcta gtagctctta atcttatctt tcttctatcc ccattaaata tatttttctt
    19621 tactattgtt tttccattta tatatattgc agtataaacc agtcccacag gcttctcttt
    19681 ggttcctcca ccaggtccag ctactccagt tgtagatata cctatattag ctttaagtcc
    19741 tttagcaacg ccctcagcca tttctatagc tgtttgctca cttactgctc caaactcttc
    19801 caaagtttct ctttttactc ctaatctttt cattttagaa tcattagaat atgttacaca
    19861 accttcaaga aatacagagg atatacctgg atagtttata agagatgaag aaaccaaacc
    19921 tccagtacaa gattcagcca ctgctattgt tagatttttt tctacaagta gcttagaaac
    19981 agcatcttct aaagcagtct ccccttcaga tatggaaata tctccttcag tatatacata
    20041 tttaccaact ctacttttta ttttttcttc tactggtttt attaatttaa aagcttctct
    20101 ttcattttca gcctttgcag ttattcttat tgtaacttct aattcttttg catataaagc
    20161 aactgttgga ttagtctgct cattaattat atcgagaatt tctaattcta gatttgactc
    20221 acctattcca tataatctta aagtcttaga tactagcatt tcatttgtga attgttgtaa
    20281 gtaaggtttg acactctcat taaacatagc tttcatttct cttggaggtc caggcaatac
    20341 tattataaat ttaccatctt tttttaaaat tgcccctgga gctgtaccat tattattttt
    20401 tagtactata gcatcttttg gaaagtacac ttgtttcata ttattttcgg ccattttttt
    20461 ccctgtttta acaaagaaag attccaaaac ctctagtgaa ggtttatgta gttctaattt
    20521 ctgaccaaag tattctgcag cagtttcttt tgtcatatca tcaccagttg gcccaagacc
    20581 acctgtagta attacaaaat cacttctctt taagctttca tcaaaacatt ctaaaagtct
    20641 ttgtttgtta tctccaacag tactctgatg gtatacttct attcccaatg atgcaagttc
    20701 tttagctaaa aattgagaat ttgtatttac tatatctccc aataatattt ctgttcctac
    20761 acttattatt tcagctttca aaatttatca ctccttgatt tatagccatt aagactagtc
    20821 ttaagttcat aacaataatt ttagcaaaaa taataacata aataaagtta aaaaatcatt
    20881 taaacatatc tttaaactag attattattc taaaaccaca cataatataa acttcgtata
    20941 tagtggtact ttcatcctca actgttaatt catataatta agtatgtaaa taaaaagttt
    21001 actttaaaat aataaaaatt aaaataggag tgtataatat gaaaatagca gttataatgg
    21061 gtggaatatc atcagagaga gaagtttctt taaattcagg aaaagagata tataataacc
    21121 ttgataaaaa taaatatgaa gttgtaaaag taataataga tgataaaaaa gatatattta
    21181 caaagatacc agaagatata gattttgcaa tacttgcttt acatggaaaa tttggtgaag
    21241 atggatgtat acaatcaata cttgagacta tggatatacc atactctgga tgtggacctc
    21301 tttgcagtgg gatgtgtatg gataaaaata taactaaaaa aatgcttaga gattcaaacc
    21361 tacctactgc tccatgggtt ttagtaaaat cagttgatga aatagactat gatgaaatag
    21421 ataatatagg ttatccagta tttataaaac caaactcagg aggttcaagt gtagccacat
    21481 tctttattca ttctaaggat gaagttgaag aagcagttag aaaaggtctg gaagttgatg
    21541 aatttgtaat gattgaaaaa tatataccag gtggagaata tacttcattc atattaaatg
    21601 gagaagtgtt ccctacaata tctataaaat ctgattcagg attctttgat tatgaagcta
    21661 agtactcagt tgaaaaaggt gcaaaggaag aagttgttta cttagatgaa gaacttcaaa
    21721 aaagagtaaa cgaaatttct gagacttgtt ggaaaatttt taattgcaaa gcatatgtaa
    21781 gagttgatat gattataagt gaaggtattc catatgttct tgaactaaat actcttcctg
    21841 gaatgactca aactagttta attcctagaa gtgctgctgc tagaggtatt aagtacagtg
    21901 aactattaga taaacttata gaatactctt taaattagat gaaataattt gaggtggtaa
    21961 tttgatattt tcaaatctaa aactaaatga taatgaacca atttatatac aacttaaaaa
    22021 ttatataagt gatatgatat caaaaggact aattccagac aatagtaagc tcccttctac
    22081 aagggagctt agtcagcttt tacaagttag ccgaaattct gtagttttaa cttatgaaga
    22141 attaaaatca gaaggattaa tctactctat ttcaggaaaa ggtacatttg ttaaatcaaa
    22201 gaataagtct tcaaatacta catggtcatt aaactgggat tgtttagaga atacatactc
    22261 taaaaaagcc aatgaattgg acattataaa aagtgaaatt ccttggagct ctgacctgat
    22321 ttcctttaaa agtatttctc cagatggaga tttattcgac atagaagaat taaaaaaatc
    22381 ctttttaaat agaatttcac tagaaggaca taaattactc aactatggat atgcacaagg
    22441 ttataagccc cttattgact atttactaga atatatgaca aataagggtg ctgatataag
    22501 taataaagat atactaataa caaatggatt cacagaaggc tttgatatta ttatgtcatc
    22561 ttttacccaa gaaaaagact acataatatg tgaaaatcct actcataata ctgctataaa
    22621 aattatgaaa tctcataacc tcaatatctt aggtgtagat ataaatgaag atggtttgga
    22681 cttcaatatg ctagaagaca atttagttaa gtataaaaat aaaataaagt ttgcatacat
    22741 tacaccctct tatcacaatc ctacaggaat tgtaatgact cctgaaaata ggtacaaatt
    22801 ctataatctt atgaaaaaat acaatatagc aattatagaa gatggattta atgaagaatt
    22861 actctacaat agttctcata tatttcctat ctgttcccta gataatatga ataatggggt
    22921 cgtatatatt ggtagttttt caaaaatttt atttcctgga atgagaattg gatggatatt
    22981 tgcagataaa aaagtaatca ataagctaga aagtgtaaaa aggtgcagaa acatacatgt
    23041 atcctttttg gaccaaggaa ttttatttga ttatttaagt aatggaggtt ttgaaaaata
    23101 cattaaaaaa atacgtaaat tctatgggga caaatttaac tttgcatata agtgtataaa
    23161 aaaatatata aaaacagaat atatcttggg tgatggtggt ttgcatatat ttataaaact
    23221 aaaaaatata gatactagag ttttattaga aaaatgttat gaaaaaaatg ttatatttat
    23281 gcctggagat ttatttttta cagataactc tggttttgat actttaagac tcggattttc
    23341 taggttgtct tttgaagaca ttgaaattgg aataaaaata attggagaaa ctataaatga
    23401 aatgttctag gttaactaaa tattgttagc ctagtttatt tttatgaggt ataatatata
    23461 tagtttatat gaatctaata ctgggggata atatgagtaa aaagtcaaaa cgaagaaaaa
    23521 ttaataaatt aaaattaacg attgtaataa ctgtattatt tcttgcttta ttatctatat
    23581 atgagatact gttctctaat cccaacaaaa aaggtaatat aaagagtcaa gaaactatgc
    23641 aaaataaaaa taatacaaca gatgattcta aaaataatca aagctctacc aaaaagcagg
    23701 aagataataa tatcaagcct actttttcat attcttcaat accagataat ataaaaaata
    23761 aaatgattgg taaatctatg cctactgatg aacctataag ctttgatagt ctatcttatt
    23821 taaaacttac atattatgga tttgatgaaa aaactcatca aggagaaatg atagtaaaca
    23881 gtgaactagc tccagaagta gtggatattt ttaaggaatt atacgaaaaa aaatatccaa
    23941 ttgaaaaaat taagttaata gatgactatg atgctgtaga tgaaaaatct atgtctgata
    24001 ataatacctc atcattttgt tatagaacta tagctggtac aaatgtagtt tcaaatcatg
    24061 gtaaaggacg tgctatagat ataaacccat tacagaatcc tcaagtttct ggaaatgatg
    24121 ttacacctaa agtaagtaca gtttatgctg ataggtctag cacaaaattt ggaatgataa
    24181 aaaaaggtga tgattgttat aatgcttttg taagcagagg ttggagttgg ggtggctatt
    24241 ggaaaaaccc agattatcaa cattttgaaa aataatagat tagaaataaa ggtgaattat
    24301 atgaatttaa tgacattgga aaatataagt aaaagctatt ctgaaaaaaa attactagaa
    24361 aatatatctc ttggaataaa tgaaggggaa aaaataggac ttattggtgt aaatggtact
    24421 ggtaaatcta ccttactcaa aattattgca ggagttgaag aaagtgagac tggtaatata
    24481 acaaaaacta atggtattag gatagaatat ctacctcaaa atccaactta caatgaaaat
    24541 tcaactgtac ttgaacaagt atttaatgga acttctgaag aattaaagct acttggtgaa
    24601 tatcaagaaa tcctagaaaa acttaattca aactttacag aggaattaaa taaaaaatta
    24661 ttatcagtac acgaaaaaat agatgcctta aatctttggg atttagaaag tgaagctaag
    24721 tcagtactta caaagcttgg tataaatgac tttaatcaaa aaatagcaga actttctggt
    24781 gggcaaagaa agagagtctc tctggcatca gcccttatta caccttgtga gtttttgata
    24841 ctagatgagc ctacaaacca tctagacaat gatactatag attggcttga ggaatatctt
    24901 aattccagaa aaggctcatt gcttatgata actcatgata gatacttttt agaccgtgtt
    24961 acaaatagaa ttattgaact ggacaaaggt agattattca gttatgatgg gaattattca
    25021 atatttttgg aaaagaaaat ggaaagactt tcgctagaag caagtattga agataaaaga
    25081 caaaatttaa taagaacaga gcttgcgtgg gtaagacgag gagctcaggc tcgtagcact
    25141 aaacaaaaag ctcgtttaca aagatttgat gaacttataa atagagattc ttttaaacct
    25201 gatgaaaatc ttgatatatc tgttgcatca agtagacttg gaaataaaat aatagaaata
    25261 cacaatatat ctaaatcatt tgaagaaaat actgttatag ataatttaga atacacttta
    25321 actcgtacag atagaattgg tataataggc aaaaatggta tgggtaaatc tacccttata
    25381 aatatactta atggagaaat agagcctgat agtggatata tttcaattgg agaaacggtt
    25441 aaaataggtt gtttttctca agacgactct catatgaata ttgatatgag ggctatagac
    25501 tatgtaaaag aagctagtga ctatatagaa acttcagatg gtactaggat tacagcttct
    25561 caaatgtgtg aaagattcct ttttaatggt actatgcaat atactttgat aggtaaatta
    25621 tctggtggtg aaagaagaag actacatctt cttaaaatcc ttatgacagc tcctaatgtg
    25681 cttcttttag atgaacctac aaatgattta gatattgaaa cgcttaaaat attagaagaa
    25741 tacctagacg aatttaaagg tgtagtagtt acagtatcac atgatagata tttcttagat
    25801 agaatttgta ataaaatatt tgcatatgaa ggtaatggaa aaattcatat ctatacaggt
    25861 aattacagtg attatcttat ttatagagag attcaaggaa ttgaatttga agaatctaaa
    25921 agggaatcag ttaaaaaaga tgactctact accccaaaaa aagaaaaacc taaaaatgat
    25981 aaaaagctta agttctcata taatgaacaa agggagtttg aaaatattga ctctgatata
    26041 gaaaaactcg aatctaaaat tgcaacactt gatgaaagta ctgcaaaatt ttcaactgat
    26101 tttactaaac tccaagaaat tttagatgag aaatctaaac tagaaaaaga acttgaatac
    26161 aaatatgaaa gatgggaata tttaaataat ctagctgatg aaatagctaa taataagtaa
    26221 tattaaaata atcttaagtt taataagttt tgctttaaat ctgtaattta taaatatttt
    26281 aaaggaagtg tctcaaaatg aactttttag ttttatgaga cactcttttt tatgagaaat
    26341 caaatatatt ttcatcattt caacaataca aaagaggctt atctctattt tgagacagcc
    26401 tctttcataa ttaccttcta atttatatgc tctaatatat ctataataat tcttattata
    26461 tctatatatc cagtctttca atatagtgac aattaacttc aatatgattt aaaaatacta
    26521 acaacctttc ataaagtgga tgagattctt ctccatattt agcttctagg ttcttgccta
    26581 tttctctaac agtcctttga ccatcaattt gtaaaaaaac aaaactggaa tattcatcta
    26641 gttctatttt tttgtattta ggtattctag cttttaactt tctaaaaaac ttttgaattt
    26701 tatggtcctg tttttcaagt atagtaacaa cacgattttc agctacttca tattcaagtc
    26761 catcagtaat tttaaataca atatctaaaa catcattact cttgctcatt attttttact
    26821 ttagataaaa caataggtaa tgctgtaagc acaactaaaa ctactaataa agcaattgcc
    26881 attgaatttg ttgcagcaaa tccacttggt acctttggag ttacaactcc agttacttgt
    26941 aatattatac ctattaaacc aatgatagaa ccaccagcaa caagaccaga agataaactt
    27001 ataccattag aaactttaac ttctttttct ttttcagttt tagatactaa ttcaacaaat
    27061 aaacgaatta atgctccaac taatattata gaagtagttg caattggtaa atagaatcct
    27121 atagctatag tcattattgg taaatttaat gcatatagaa ctattgccat aaatactcca
    27181 acaataatca taacccaagg taaactacct gacataatac cagaagttaa tgttgacatt
    27241 aagtttgctt gtggtaatgc aaattgtaca ttatcaccag ttgctctaag ttggtcagaa
    27301 agtaataaaa ttactcctac aacaactata acaccaacta tacttgcaat tgtaaagtaa
    27361 cgttgcattt cgtttttgct accaccaata atataagtta ctttttgaga ttgagtataa
    27421 cctccagcta tagcaatagc aacaactata aatgaaccaa ataaaagtaa tgatttatta
    27481 gcttctaaat cagtccatcc cattataaca aacactaatg ttacaataac tagagaagct
    27541 atagtcatac cagaaactgg aagatttgat gttccgattg ttcctgttaa acgaccagct
    27601 actatgacaa ataataaaga taataaaaga gatatgatag ctccaataat tgccataaca
    27661 atgttaccag aaattaagaa tgctgctaaa aatccaataa ctactccacc taataaaagt
    27721 atcatttgta tagatgaacc ttcttctcct tctccagcac ttgatttagc tttaagtgtt
    27781 tcttttatag aagcgattat agttggtata agttttattg caccaataat acctccacaa
    27841 agcatcatac cagcacctat atattttaca taactactag atattgctcc aacatccatt
    27901 tggttaagag gcattgcagg attattccat accatagctc catctttagc catatctgta
    27961 aagtatccta ttaaaggtgc tataccaaag tttgataaaa tacttcctgc aaacattgtt
    28021 aaagaaactt caagccctac tataaatcca attcctaaaa gtagaggatt tacttcaata
    28081 tcaaatttcc atttgtagaa tttgcttcct acaagactca ttacattgtt tgctacattt
    28141 aagaatgaac cagttaatac agtaataaat ccacttatta caaatccaat tcccatgtac
    28201 ttaattgaat ctccaccagc ctctgaagca acaagtgtct cagatatagc cattgattct
    28261 gggtacataa gtttaccatg ttcttcaact attagatagt tgtgaactat agcagcaatc
    28321 cctataccaa ataaaactcc accaactcca acagctaaac cttctaagaa tgtaatttgg
    28381 cttccaatta atataactgc tggtaatacg aaaataaatc cacttgcaac agattctcca
    28441 ccacttgaca taccttggat taagttttta ccaagaatac cctttgaacg agcaaatgct
    28501 ccaacaaaag cagaacctat tatagctcct ggaatacctg cggcaactgt aagaccggct
    28561 ttcattccag agtaagttgt agatgcggca aaaatggcag ctaaaataat accgataatc
    28621 agtacagcta catttccacc ggttctggac ttatctgtga tatatggaac gtaatctttt
    28681 ccactaacct caccataagc acccttaggt aattttttgt tcataaaatt ttcacccctt
    28741 ttcaatttgt atatattata acatatatac atataaaact taacttttta ttattttaca
    28801 aatagcgagt caaaactact a
//
LOCUS       DAGSRM010000039        28236 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.039, whole genome shotgun sequence.
ACCESSION   DAGSRM010000039 DAGSRM010000000
VERSION     DAGSRM010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28236
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.039"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(31..546)
                     /locus_tag="MJE33_001950"
     CDS             complement(31..546)
                     /locus_tag="MJE33_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HBY9647884.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDIAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
     gene            complement(909..1979)
                     /gene="leuB"
                     /locus_tag="MJE33_001951"
     CDS             complement(909..1979)
                     /gene="leuB"
                     /locus_tag="MJE33_001951"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HBY9647885.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDETGEPLPNKTVETCRKNEAVLLGAVGGPKWDKCKGDKRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELS
                     HMYVDNAAMQVVKDPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLREDSFGMY
                     EPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTID
                     IYNGVGNVVGTREMGELIVNEI"
     gene            complement(1986..2477)
                     /gene="leuD"
                     /locus_tag="MJE33_001952"
     CDS             complement(1986..2477)
                     /gene="leuD"
                     /locus_tag="MJE33_001952"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HBY9647886.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDK
                     FISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIDEGDNIEVDFSTGVIKNITKGKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            complement(2492..3769)
                     /gene="leuC"
                     /locus_tag="MJE33_001953"
     CDS             complement(2492..3769)
                     /gene="leuC"
                     /locus_tag="MJE33_001953"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HBY9647887.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIR
                     EFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTMEYINSHKCSTMTKDVNIYEADEDAVYDEVYEIDLAELKETV
                     AFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAITGKISKPSEII"
     gene            complement(3726..5468)
                     /gene="leuA"
                     /locus_tag="MJE33_001954"
     CDS             complement(3726..5468)
                     /gene="leuA"
                     /locus_tag="MJE33_001954"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HBY9647888.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RAHLIEKTFESIRGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTPQKKVIINLPSTVEMATPNV
                     YADQIEWFCKNISCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRHPYAGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARISYMGNEQRIVGIGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEIK
                     NGDDYDAENISQTC"
     misc_binding    complement(5592..5838)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(5986..6432)
                     /locus_tag="MJE33_001955"
     CDS             complement(5986..6432)
                     /locus_tag="MJE33_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647889.1"
                     /translation="MSKNKFIYMNYYDNGYFKKGMTIYMVLALIILATIFIYSTTILY
                     ILCIITAVFLFKQGRDFSNKYEGKVYITLDNHSILINNQCIFSIKSKQSQKFNYKIIE
                     KIEVVKNILNIYTDESNYKIRLRALSLEDEKKLLNIIDEKMKKFKA"
     gene            complement(6453..7172)
                     /locus_tag="MJE33_001956"
     CDS             complement(6453..7172)
                     /locus_tag="MJE33_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBY9647890.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASKKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPREVQGVKILRGIEGNIMDYNGNLDVQEDMLKHLDYIIASL
                     HRPCIASGTKEKNTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSHMLTLCKTYGARVILGTDSHICYSIGEFENAEKVLEAVDF
                     PDELVINYHEDEIIEFFDINF"
     gene            complement(7290..8564)
                     /locus_tag="MJE33_001957"
     CDS             complement(7290..8564)
                     /locus_tag="MJE33_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBY9647891.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAKLQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDVRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RIKAAKEDLFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNAKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
     gene            complement(8764..10131)
                     /locus_tag="MJE33_001958"
     CDS             complement(8764..10131)
                     /locus_tag="MJE33_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBY9647892.1"
                     /translation="MENSNTNLGSESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIITACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWMFGASESTIGYSMDYLNIYLIGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIILPVLGLGISPFIMQSTESLVLIALNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCFTYSTILWL
                     LLMIFPAFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISFFIFYKCFLSKP
                     KAIKE"
     gene            complement(10381..10845)
                     /locus_tag="MJE33_001959"
     CDS             complement(10381..10845)
                     /locus_tag="MJE33_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HBY9647893.1"
                     /translation="MSLRIGGNSLGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVY
                     VFSVIGSTLVSIPLILTFRHVLQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGII
                     LFVGIPLPTTGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            complement(10850..11479)
                     /locus_tag="MJE33_001960"
     CDS             complement(10850..11479)
                     /locus_tag="MJE33_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBY9647894.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNKTFKTIIKLPYIIF
                     ILFMYLIRMIPLKKAKEAIFYPIVDFSEEDLKKFFDDCIMKKINESMKKVIYKNKEED
                     NVIIMITASPYAYMKYFKYYGLADEVIGTEFFYENSRYKNQFIGENCKGIEKLKRIKA
                     VLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKDGEVIEQINS"
     gene            complement(11513..12400)
                     /locus_tag="MJE33_001961"
     CDS             complement(11513..12400)
                     /locus_tag="MJE33_001961"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HBY9647895.1"
                     /translation="MKQTNTSIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLFLNTFIAFVCFCLMSSSVYALNDAIDMDKDKKHPKKCNRPVASGRISKKSANILF
                     VVLALISVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRDDAVEHRKILEDYDIENLNQMM
                     IVILSSTIVCYALYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNENNPTDIVLKDN
                     ALKINVILWIITCLIILIF"
     gene            complement(12429..13667)
                     /locus_tag="MJE33_001962"
     CDS             complement(12429..13667)
                     /locus_tag="MJE33_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647896.1"
                     /translation="MDGDILMSKILKYKNEMFLFILVVITYLIITKIFFSKTTIFYDL
                     NNTYDVLLDTDTGVLFNLNVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTD
                     FNSAYGFGLICLQIITSAMSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFMTLNV
                     ERFIYSQFSLIFFIVIANKMKGKNSYLVELAAIPLFGITISNIYLYFFNMIFEFKLKI
                     GKMLKHLITFILMAYICVVSTKSYESFMNLGNVIQYDTKFISGEPILEKIAMIIERLL
                     YSVFYFPGASIKKGLFLQNGEVATIPVILTLLALCFCVLSVIENSEKRVPKLCMGIII
                     FNLTLHGIVGYNLVNSSIMAINFSFAVIILLAYFTKALRKNEKNMYNIFLSLLLVTIV
                     ISNVNGFIEILNIGIKSYPV"
     gene            complement(14051..15193)
                     /locus_tag="MJE33_001963"
     CDS             complement(14051..15193)
                     /locus_tag="MJE33_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBY9647897.1"
                     /translation="MRYVEYGKTGKMVSVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMRPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKFEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLSGGTIPKYNKELSF
                     LSKDGESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDIHDIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANSVKV"
     gene            complement(15334..16200)
                     /locus_tag="MJE33_001964"
     CDS             complement(15334..16200)
                     /locus_tag="MJE33_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBY9647898.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVLR
                     IANPVVPAITIESLIALIITLFINIFVCMYEYRIGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLVGVKLGFPVIIESIVPIIISAFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEQSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
     gene            complement(16534..18081)
                     /locus_tag="MJE33_001965"
     CDS             complement(16534..18081)
                     /locus_tag="MJE33_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBY9647899.1"
                     /translation="MANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGIII
                     SRISSLRKFVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNTYI
                     GITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPKKN
                     YIALKVISVVVVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNTNL
                     KVKKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMKHAPIKDADEAYNVVKDTME
                     KENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENREDG
                     LVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDKNF
                     FVPYYAAPLVREDTLKKYPELEKVMNMLSGKINEETMRELNYQVDELGKSPEEVAHSF
                     LVKEGLV"
     gene            complement(18074..19210)
                     /locus_tag="MJE33_001966"
     CDS             complement(18074..19210)
                     /locus_tag="MJE33_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9647900.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLVVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSDEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDIDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
     gene            complement(19480..20577)
                     /gene="dinB"
                     /locus_tag="MJE33_001967"
     CDS             complement(19480..20577)
                     /gene="dinB"
                     /locus_tag="MJE33_001967"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBY9647901.1"
                     /translation="MKHYRKIIHIDMDAFYASIEQRDNKKLKGRPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKNIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLRK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVEPNRVRKSVGAETTLSHNLDIDEEETRNILDELCEEVCH
                     RLKNSEKFGKTLTLKIKYEDFTKITRSLSLEHYIDEYNDIRSGVDNLLRNVEVNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQM"
     gene            complement(20967..22472)
                     /locus_tag="MJE33_001968"
     CDS             complement(20967..22472)
                     /locus_tag="MJE33_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBY9647902.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHENKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAVV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMEKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
     gene            complement(22734..23084)
                     /locus_tag="MJE33_001969"
     CDS             complement(22734..23084)
                     /locus_tag="MJE33_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647903.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGNVNEAEEILFDKAKSVNSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRKVLNLE"
     gene            complement(23307..23498)
                     /locus_tag="MJE33_001970"
     CDS             complement(23307..23498)
                     /locus_tag="MJE33_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647904.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            complement(23616..24542)
                     /locus_tag="MJE33_001971"
     CDS             complement(23616..24542)
                     /locus_tag="MJE33_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBY9647905.1"
                     /translation="MRKIVLKDILFLVTTLSIIVILLLVTNITTNSKIKSTSESYLKS
                     DKNSVRQNGIQRSTKSSTKNTAFKTTILNTGKSDCILVEIGNKVIMIDTGEDKNGKQI
                     VDRLKEKGINTLDYLILTHLDKDHIGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDS
                     LKKADIEPVLLKDNMNIVINGAEINIHPASKSKYESSNDYSIITNISYGAHKFLFAGD
                     AEEERLSEFINGNTLKYDFVKMPHHGRYDKLTETFLESISPQYAVITCSEKKEPEEGV
                     LKILERLNIKTFLTSNGEVVINSDSKTLSVNQ"
     gene            complement(24687..26183)
                     /locus_tag="MJE33_001972"
     CDS             complement(24687..26183)
                     /locus_tag="MJE33_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBY9647906.1"
                     /translation="MFEFETQLKRLKHEVLTAVARLSIEENLTKENIEKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVSLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACMSACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFVDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKMFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEKSLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSVQNYIAEKGLSFDFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKAKNTFDRKNGQSTKKSVLSNDKIKEFEDVNLER"
     gene            complement(26463..27953)
                     /locus_tag="MJE33_001973"
     CDS             complement(26463..27953)
                     /locus_tag="MJE33_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBY9647907.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDTMDIEEDEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEDKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMEKGEAFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANTKIKDFSNINLEK"
BASE COUNT         9206 a         5156 c         2895 g        10979 t
ORIGIN      
        1 tatgttttaa aatgtaaaat ttttatttaa tcaaagttca atatattcca ctttttcttt
       61 cgattcttca aaaaaccatt cttcattaga aaaatcacct tctgcaattt gtccaatagg
      121 cattttcata tactttccag ttgctgtcac tgctatatct ccatttggta gaattatttc
      181 tcctgtacct tcaaataatt ttctgttttc tttagttatt cttccaacaa cttttatagt
      241 agcatctgtt ggtactggtt tcttatattt caactctaaa gatactgtta ctccccatat
      301 ttggtcatca tttatactta cagctcttcc aatagtctca tccaatattg cagcagacat
      361 acctccatga actctcccag gataactttg atgccaatcc tttgtattac aaattgatac
      421 aagctctcca ttttcaagtt cataaaaatc tgctttaagt cctagctcat tttgtgttcc
      481 acaaactaaa caagatttac tactattttg cttcttcaaa actttatact ttgccaaaaa
      541 atccatctcc taaccgacat tagtagtcgc ttaatattaa ataattctaa aatatattat
      601 ataatattaa taaaatccct gcaaacttct aataataaaa ataggctact tcaatatgat
      661 aagtttaatt taaagtatta ttttccaaaa aataacttta tcaaaataga aactcaaagt
      721 agatataagc ctctttttcg ttattaaaat agtaaaaata tattttactt aatataaaaa
      781 agagtgtctc attaactaat aaagtttatt ttgagacacc cctcataaaa caaattatac
      841 tacaatagat ttttactaat tctacaagtt atatattcaa attatattat tcaaattaaa
      901 ctataattct aaatttcatt tactattaat tcgcccattt cccttgttcc tacaacattt
      961 cccacaccat tatagatatc aatagttcta taacctttat tgagtacact ttgtacagca
     1021 tcttcaatac acttagcttc ttcttctaag ttaaaactat gtcttaacat catgcttaca
     1081 gatagaatag tcgcaattgg atttacaata tcttttccag ctatatcagg tgcacttcca
     1141 tgtattggtt catacatacc aaaactatct tctctaagtg atgctgatgg taacatacct
     1201 atagaacccg taatcataga tgcttcatca gatattatat ctccaaatat attattagtc
     1261 actattacat caaattggct agggtctttt acaacttgca tagcagcatt atcaacatac
     1321 atatgtgaca gttctacatc ttcaaactct ttagctaagt cttccattat acttctccaa
     1381 agtcttgaac tctcaagcac atttgcctta tctacactta taagtttttt atttctattt
     1441 ctggctattt caaaagctct cttaccaatt cttcttattt cattttcgtt atatatttct
     1501 acatcataag cattttcgat accatcaatg atttttcttc cacgttctcc aaaataaatg
     1561 ccccctgtta attcccttac aacaacaaaa tcaatgcctt tctcaactat atccgttcta
     1621 agtggtgaag cttcttttat actttcatac attgtagctg gtcttaaatt tgcaaataaa
     1681 cccaatgact ctcttaattt taaaagacca gactctggtc ttttatctcc cttacattta
     1741 tcccactttg gtcctccaac agcaccaagc aatacagctt cgttttttcg acaagtttca
     1801 acagttttat taggtagagg ttctccagtt tcatcaatcg cgcatcctcc agctaataca
     1861 tattcacaat cgaatctatg attaaattta caacaaattt tatttagaac ctttatacct
     1921 tcatctataa tttccggtcc aacaccatca ccttttatga ctgctatatt acaattcata
     1981 cttcactacc ccctatttgc ttttattgag ttaattaagc cttcatttaa tataatattt
     2041 tgcataaact caggaaatgg ttctgcttta tattcttttc cttttgttat attttttatc
     2101 acacctgtag aaaaatctac ttctatatta tccccttcat ctatgttatt ggcagcttct
     2161 tcacattcaa gtataggaag ccctatattt atagagtttc taaaaaatat tctagcaaaa
     2221 gtagaagcaa tcacacagga tactccactt tcttttatta caattggtgc atgttctctt
     2281 gatgaaccac aaccaaagtt tttagttgca actataatat ctcctttttt taccttagaa
     2341 ataaatttat catcaatatc ctccatacaa tgcgttgcta gttctttata atcagctata
     2401 tttaaatatc tagctggtat tattacatct gtatctatat tgtcaccgaa tttaaaaaca
     2461 cttccattgg caatcataaa ttttacctcc cttaaattat ttctgatggt tttgatattt
     2521 ttccagttat agctgatgct gctgctacag ctgggcttgc aagatacacc tctgaatcaa
     2581 catgccccat tcttcctacg aaattcctat ttgttgttga aatggatttt tctcctgctg
     2641 caagtatacc catatgtccc cctagacatg gaccacaagt tggtgtagaa actgctgctc
     2701 ctgcctctat aaaagtggta atataccctt tttctatagc ttcaagatat actttttgag
     2761 ttccaggaaa tatcataact ctaactccat ctgctacttt tttgcctttc attatctcag
     2821 ctactacttc aaggtctgat attcttccat ttgtacaaga gcctattaca acttggtcta
     2881 tctttatatc cttatctatc tcatctactg ttctagtatt ttcaggaaga tgaggaaagg
     2941 ctactgtttc ttttagttca gctaaatcta tttcgtatac ttcatcatag actgcatctt
     3001 catctgcttc atatatattt acatcttttg tcatagttga acacttatgt gaatttatat
     3061 attccatagt cttatcgtct actggaaaaa ttccattttt tgctcctgct tctattgcca
     3121 tattacaaat agtaaatcta tcatccatag ataagtattc tactccatct ccacaaaatt
     3181 ccattgactt gtaaagtgct ccatcaacac ctatctcccc tattatatga agtattatat
     3241 ctttaccact tatccattta tttggtttat tcttcaaaac aaattttatt gctccaggaa
     3301 ccttaaacca acattttcct gtagccatac ctgctcccat atcagttgaa cctacaccag
     3361 tagaaaatgc tcctaatgct ccatatgtgc aagtatgaga gtcagcacct ataattacat
     3421 ctccagcagt tactatacct ttttcaggaa gaagaacatg ctctattccc atatctccta
     3481 catcaaaaaa atttacaact ccttttgatt ttgcgaaact tcttgtaaat ttacattgtt
     3541 ctgcactctt tatatcttta ttaggtgtaa agtggtctaa aaccatcact actttagtct
     3601 tatcataaac atcttcaatt cctaattttt caaattctct aatggctact ggtgatgtta
     3661 tatcattacc taaaactaca tctaaatctg cttcgattaa ttgccctttc ttaacttcat
     3721 ttaatttagc atgtttggct aatattttct gcgtcatagt catccccatt ttttatttcc
     3781 tcctcaatat aacttttatt tattgcattc ataagtgcat taatagctgc tgtatttatg
     3841 ttttccgata tacttacacc aaaatagact tcattattgt gctctattcc tacataagct
     3901 acccctcttg aatgtgaccc ttcctctaat gcatgttccc agtaatatct aaatttataa
     3961 tctttcatgc cacattgctt tagtgcatta ttaaatgaat ctacaggtcc atttcctata
     4021 ccaacaatcc tttgttcatt tcccatataa cttattcttg cagtcatctc aatcatgtta
     4081 gtatcattag aatcatcatt ttcatagttt aattcatcca ttgattttat tttatacttc
     4141 ttaactttat aagggctctc aacttcaata tattctttct taaacaaatt aaatattgct
     4201 tcactactaa gttcttcccc aattctatca gattccattt tgactacatt tccaaaatca
     4261 gaatgcatat tcttaggtat cataaatcca taatctgttt ccattatata aacagctcct
     4321 ccttttccag attggctatt tatccttata atttctttgt attctcttcc aatatcatga
     4381 gggtctattg gtaaatatgg aacttcccag taatcattat ctctgttttt cattgctatc
     4441 atgccttttc taatagcatc ttgatgtgaa cctgaaaaag cacaatgtac aagattacct
     4501 gcatatggat gtctatcatg taccataagt tttgtacagt cttcatatat acctattatc
     4561 ttatctatat tgctaaaatc tagctctggg tctattcctt gtgtataaag attaagtccc
     4621 acatttacta tatccatatt cccagttctt tcaccatttc caaatagtgt tccttcaagt
     4681 ctatcggcac cagccaataa tccaagttca cttgcagccg tacaagttcc tctatcattg
     4741 tgagtatgca aacttagtat aatagaatcc ctacaactta tatttttaca aaaccattct
     4801 atttggtctg catatacgtt tggtgttgcc atttcaactg ttgatggaag atttattatt
     4861 actttttttt gaggagttgg tttccaaaca tctatcactg cctcacatat ttcaagtgca
     4921 taatccatct ctgttcctgt aaaactctct ggtgaatatt caaaagtaaa ttctgtttct
     4981 ggatattttt ttacttcttc attaaataat tttgctcctt caactgctat atctattatt
     5041 tcttgtttac tcatattaaa aaccacatct ctttgaagaa cagatgtaga gttatataaa
     5101 tgtataattg cttttttaca tccccttatt gattcaaatg ttttttctat aagatgagcc
     5161 ctagattgtg taagtacttg tatagttaca tcatctggaa tcatattctc atctattaat
     5221 tttcttaaaa atgtatactc tgtatttgat gctgatggaa atcctacctc tatttcttta
     5281 aatcctgtat caataagcat tttgaacatt ctgacttttt catttacact cataggtgtt
     5341 ggcagtgact gatttccgtc tcttaaatcg acactacacc aaattggagc cttaaatatt
     5401 tcattatctg gccatttacg atttactatt tgcattttat catatttttt atactttcca
     5461 cacttcatgt tatttccccc tttttttgtt ttttgtattc agtatacctc atatcgacgc
     5521 aatcctgcac aattcataag gataaatgtc aattttatca ttagaataaa aaaaacctca
     5581 tctctaataa aattagagac gaggttttgc tcgcggtacc actctattta gctttctaaa
     5641 aagctccctt agtaatagcc aatacaataa ttataatgac tatatttcct ataacgtgga
     5701 attactccga ttattctaag atttaaatct tagaaaacag ctcctagaca agttcagaac
     5761 tatcaactac taaatcacac catcctttag ctctctgaaa gtttcattgt tcttactact
     5821 tctattcatc acttttttaa tcatttaaat tttctaaata ttttgattta atagttatta
     5881 tatacacatt aaaatgtatt gtcaacacaa aaaataatta tctcaatttt ttatttttga
     5941 taagcaattc tataaaacat gctaactatt gaaaacagta tgatattatg ccttaaactt
     6001 tttcatcttt tcatctatga tattcaataa ttttttttca tcttccaaac tcaatgccct
     6061 taatctaatt ttataattag attcgtcagt gtatatattt aatatatttt ttactacttc
     6121 tattttttct attatcttat aattaaactt ttgactttgt ttagatttta tactgaatat
     6181 acattgatta tttattaata ttgaatgatt atcgagagta atatatactt ttccctcata
     6241 tttatttgaa aaatcacgac cttgtttaaa tagaaaaact gctgttatta tacataaaat
     6301 atataatatt gtcgttgaat atataaaaat tgtagctaaa attataagtg ctaaaaccat
     6361 atatatagtc attccttttt taaaataacc attgtcataa taattcatat agataaactt
     6421 atttttactc aaattttttc actcccaaga ttctaaaaat ttatatcaaa aaattctata
     6481 atttcatctt catgataatt tatgactaat tcatctggaa aatctactgc ttctaaaact
     6541 ttttctgcat tttcaaattc tcctatagaa taacatatat gagagtcagt accaagaatt
     6601 actcttgcac catatgtttt acacaatgtc aacatatgac ttacattttc ccaagtaccc
     6661 gttctatgag agtttgaact taatgatgaa ttatttattt ctaaaaggat atttttatct
     6721 ttagcttttt ttactatagg ttcataatct aaaggatacc tactgtcatc tggatgtcca
     6781 attattttta ccttaggttt atccataact tttaaaatag catttgtatt tttttctttt
     6841 gtaccagagg ctatacaagg tctatgcaaa cttgcgatta tataatccaa atgtttcaac
     6901 atatcttctt gaacatctaa attaccatta taatccatta tatttccttc tattcctcta
     6961 agaatcttta cgccttgcac ttcacgtgga agcaaatgca aattataaaa atagaatgga
     7021 tgtggtcctc ctggcatatt aggtccatgg tctgatattc ctaagtattt tatattcttc
     7081 ttactcgcct cttcgacatt ttccttgaaa gtactatgtg catgtccact tattagagtg
     7141 tgacaatgta catccataat agactttttc attaaatttc ctcctaatat tttacataaa
     7201 ttcgtgaaaa tagctaaagg gctatttcaa aactaaagct gtcataatag acagtgtaac
     7261 gttttaaaac agccccattt tcaattattt tacacttcta aatctttaaa tttatcttta
     7321 aataattctc caatacttcc taaagattcc tctacttcta ggtattctgt tatatcctct
     7381 tctggctctc tagttgcttc ttttattgaa accaacattc ttttagtctt tggtttaaaa
     7441 tcaagaatct taactttaac ctcatctcca atattaacaa cattagtaac tttagccact
     7501 ctagtatcac tcaattcaga tattggtaaa tacgcatcta caccttcttt aatttgaaca
     7561 aaagcccctt tttctattat tctaagaact ttagcattga caacatcatc aatttgtact
     7621 tcttttgcta ttaaatccca agggtcatta tttatgtctt ttaatgccaa ggcaattttt
     7681 ttagtctctt tatttacatc caacacataa acttctactt cttgtccctc tgaaacaaca
     7741 tcttcaacac tttcaactct tgtccaagct aaattattta tatgtgctaa tccttcaatc
     7801 ccaccaatat ccacaaatgc accataaggc attatctttg tcaccttacc atctcttttt
     7861 tgtccaactt caagagaatt aaataagtct tctttagctg ctttgatacg agctttttct
     7921 tcttctcttt cttgcttaat tcttgctctt tcagcttctt tttctgcctt aatttgagct
     7981 cttctttctt ctctctcttt atttatacgc tcttgtaata catctctatg agatgctaca
     8041 agtctatttt ttctaacatc caattctttt atataaactt ctaagttaag accaacaaat
     8101 ttagatgtat ctgtaataaa ttttgtatca agttgagaaa ttggtataaa aaatgtatat
     8161 gtattgtaat tagcaaatac acctttatct atattctttt caacatttac agttattatt
     8221 ctatgttcat cataagcctt ttgtaacttg gcaaaatcat ttcttttgtc aagttgtaat
     8281 ttagataaag ttattgtacc atctttttga gatacttttg ttattacagc agttatttca
     8341 tcacctatgt ggtatatatc ttctatatat tgattttttt ctatgttcaa ttcacttata
     8401 ggtattattc catcaaaccc atagtctaat cctaattgta cttcatcatt tctcactgca
     8461 gttatttttc cagttgtcaa ttctccaact ttcactttgc tgaatacttg ttcttgctgg
     8521 tcaagtaatt cttgcattgt taaatcattt tccattataa acatcctttc ctcttattga
     8581 ttatcatatc tgtaaatatt ataccattaa tattgcaaat acttacattt ttcttactat
     8641 aatttttaaa ataaattcaa cttcatatat ttatacatta tagaaaatat cttattaaat
     8701 taaaatacaa attctaattt ttatctaata aaatattttc ataaaaaatt tatattgtat
     8761 atcttattct tttattgctt taggttttga tagaaaacat ttataaaata taaagaaaga
     8821 aattgctgtt atagtagcag ctgttatatc tgcaactggc tctgctaaaa ttactgcaaa
     8881 aagtttttca ttaaagaatg ttgggagtat aaatattaaa ggtattaaaa gtacaatctt
     8941 tctaagtaga gctaaaacca aagatattgt tgcttttcca agtgctaaaa atgtctgttg
     9001 acaagctatt tgaatcccaa acataaaagc accagcaaaa tatattttta tactccaact
     9061 agtcatacta agtaactgcg gattcttatt aaatatacta acaaaaaatg caggaaatat
     9121 cattaataaa agccataaaa tagtagaata tgtaaaacat gacaacaaac acaacttaaa
     9181 tgtctttttt actctatcaa gttgacctga accataatta taacttatta ttggttgtgc
     9241 tccttgtgta acccccatat taggaagcat aagaatttgc attatactgc tcattatggc
     9301 cattgaacct actgctaaat ctcctccata catttgtaat ttactattta aggctataag
     9361 tactaaactt tctgtagatt gcattataaa tggagagatt cccaaaccaa gcactggtaa
     9421 gatgatactt gcttttggta tcatatactt cttttttatt ttaagtatac ttttctttcc
     9481 aaagagaaac ataagtaccc aaatagcaga aacgccttgt gcaattatag tagctaatgc
     9541 agctcctttt acacccatat taaagccaaa tataaaaatc gggtcaagta ttatgtttat
     9601 taaagcacct atagcaactg tcatcattcc agttgtagca aacccttggg tatttataaa
     9661 agtattcata cccattgaaa tctgaacaaa cactgtacct attaaataaa tatttaaata
     9721 gtccattgaa tatccaattg tagattcact tgctccaaac atccaaagta aaggctcttt
     9781 tccaatctta aaacctatag tcaaaagtat tgctaaaatc accagtacag aaaaactgtt
     9841 actcattatt ttctctgcct catcattgtt ttgctcaccc atttttatag cagctaatgg
     9901 acctcctccc ataccgatta aataactaca tgcagttatt ataattatta ttggaaatgc
     9961 cactccaaca ccagccatag caacttctcc attttccatt ctaccaataa aaatcctatc
    10021 gactatgttg tataaaacat ttacaatttg tgcgataata gcaggtgtag caagtttaaa
    10081 taataattta cccacacttt cactacctaa gttagtgttt gaattttcca ttttaaaacc
    10141 tcctcaatcc aataattaaa atgtatcact aaaattttat tctatataaa aatttcattt
    10201 tattagcaga caaatttaga gtactttccc tacaagtaat actatactat atttcttttg
    10261 ccatagtaaa cattttagaa ctaactgaag ttaaatactt tatctcattt tttatttatt
    10321 attaaaaatt tttcacacat actcataaaa aaactatcca agtatattac ttagatagtt
    10381 ttatattata tgtaaagaaa gagctgaaac tataacccct gataacaaat taccaagaaa
    10441 tactcccata aaagcatctt ttattctcat ttttaatatg gaagctatag cagatgcagt
    10501 ccatgttcct gtagttggca gtggtatacc tacaaatagt attataccaa ttatcgaaac
    10561 actttttaat ttcttcattc ttgagtttat ttttctatca attacatcag caatcccttt
    10621 aaatagtttc ttccctctta aaaattgtaa tacatgtctg aatgtcaata tcaagggaat
    10681 cgataccaat gtagaaccta ttacactaaa tacataaacc catataggat ttaagcccat
    10741 tgcaatacct ataggaatgg ctcccctcaa ttctgttaca ggaaccatag ataaaattaa
    10801 tatgtgtaaa tatcccaaac tattccctcc tattctaagt gacattaagt tatgaattta
    10861 tttgctctat aacctcacca tcctttttac taactaaaaa tgcattttta gtcaatgaaa
    10921 gcattggtaa atcactctta gagtctgaat atgcatatga attttcataa tcaatctcta
    10981 tcccaagttt acctaataca gcctttattc tttttaattt ttcaattccc ttacaatttt
    11041 ctccaatgaa ttgattttta tatcttgaat tttcataaaa aaactccgtg cctataacct
    11101 cgtctgcaag accataatac ttaaaatatt tcatatatgc atatggagat gcagttatca
    11161 taattataac attatcttct tctttatttt tgtaaataac ctttttcata gattcgttaa
    11221 tttttttcat tatgcaatca tcaaaaaact tttttaaatc ttcttcacta aaatctacta
    11281 ttggataaaa gattgcctct tttgccttct tcaaaggtat cattctaatt aaatacataa
    11341 atagaataaa aataatgtat ggcaacttta taatagtttt aaaggtttta ttttttaaag
    11401 agtaaataaa aaaatctatc atgctatcag tatttataac agtataatct acatcaagta
    11461 ataatacact ttttttcata agtatttccc catcctatca ctaaaaatat cactaaaaaa
    11521 tcaatattat taaacatgta attatccata agattacgtt tatttttaaa gcattatctt
    11581 ttaggacaat atcagtagga ttattttcat ttgtagtgtt aattatataa ttatatctaa
    11641 gtacaccata aactacaaat attgttgtaa atatcatatg tggcttttct ggattacttg
    11701 tagaatacaa tgcataacat acaattgtag aacttaatat aactatcatc atttgattta
    11761 gattttctat gtcataatct tccaatattt ttctatgttc aactgcatca tctcttagag
    11821 tttctatttc ttttttacgt tttccaagag ctaagtacag agataagaaa aatgtacata
    11881 aaattatcca atttgataaa ctaacactta tggctacaca tcctgcataa accctaagta
    11941 taaaaccaag agatattgaa aacacatcta atagtattat attctttagt ttaagagaat
    12001 ataatatatt gtttatcaaa tagagactga gaattataga aagattaaaa ctaacaactg
    12061 ttgataaaca gactgaaatt aaagctaata ctacaaatag tatattagca ctttttttgc
    12121 ttattcttcc acttgccact ggtctattac attttttagg atgtttttta tctttatcca
    12181 tgtctatagc atcattcagt gcatatactg atgatgacat taggcaaaaa cacacaaacg
    12241 caataaacgt atttaaaaat aaatttatat ttagtatgtt gtttgaaaaa attatagctg
    12301 aaaatacaaa gaaattcttt atatattgct ttattctcat taactttata taatctgaaa
    12361 ttttagtaag aagccttata cttgtattgg tctgtttcat aaacttacta ccctttcaat
    12421 attatttttt aaacaggata tgattttatc cctatgttta aaatttctat aaatccattg
    12481 acattactga ttactatagt aaccaataac agcgatagaa atatattata catattcttt
    12541 tcattttttc taagcgcttt cgtaaagtaa gccaataaaa ttataactgc aaaactaaaa
    12601 tttatagcca taatacttga attgactaga ttatatccta ctataccatg taaagttaga
    12661 ttgaatataa ttattcccat acatagctta ggaactcttt tttctgaatt ttcaataact
    12721 gataaaacac aaaaacataa agctaataat gtaagaatta ctggtattgt agcaacttct
    12781 ccattttgca aaaataaccc ttttttaatg cttgctccag gaaaataaaa aaccgaatac
    12841 aataatctct ctataatcat tgcaatcttt tccaaaattg gttctccact tataaatttc
    12901 gtatcatact gtattacatt tccaaggttc ataaaactct cataagactt cgttgaaact
    12961 acacaaatat atgccattag gataaatgta attaaatgtt ttaacatttt tcctattttt
    13021 aatttaaact caaatatcat attaaaaaaa tacaaatata tattactaat agttattcca
    13081 aatagaggaa tagctgctaa ttctactaaa taagaatttt ttcctttcat cttatttgca
    13141 ataactatga aaaatattaa tgaaaattgg ctatatataa atctctctac atttaaagtc
    13201 ataaatagct gtggaaaaga aaatatcatt atcatagtaa gtaaaattaa agttaatctc
    13261 tgcattttta tctttttaat atgattaaag actaaagtta ttgacatagc actagttatt
    13321 atttgtagac aaattagtcc aaacccatag gcactattaa aatctgttgt cccaggattt
    13381 gctattgaag tacaaaatag gtatattgga tatgcaaaaa ttgatataat tgctgaaaac
    13441 aatatgtgct tactattgtc ttgacttata gcaaatacat ttaaattgaa taaaactcct
    13501 gtatctgtat ctaacaatac atcataagta ttgtttaaat cataaaatat agtagtctta
    13561 gaaaaaaata tcttagttat tattaagtaa gttattacaa ctaatataaa caaaaacatc
    13621 tcgtttttat attttaatat tttactcatc aatatatcac catccattca ttatatagga
    13681 tttgctgaca cctacaattt attccttata tttaatagcc atcttcataa tgttcttact
    13741 tgttttattg taattgaata actctttatt tacaaccaca atatttactt tattgatgtt
    13801 ataatcagtc aagtattgtt tatataacat agattagtgt tgtataaaat gagaatttgt
    13861 aagcaatatt aatgtaaaat atttgaaaaa ctttatataa aaatatacaa tgttgcttat
    13921 ggaattaaac tataacaaat taaagcataa tttaaggaga caaaattata atctgtctcc
    13981 ttaaatatta aaactatatt ttaaaacatt actaaatact atacaaattt caaatatagt
    14041 ataaaattaa ttagacttta acactattag ctccatcttc ttcccatttt ttaatttctt
    14101 ttaatctatc tattataggt aaatgctgag tacattcaac ttcacatttt ccacaagata
    14161 tacattcttt agcttttgcc ttactattca ttgtgtaatt ccaatgtcct aagccataaa
    14221 ctaactttgt catttcttca tcactctttt taaacatctg cttttcatta taaaaaagca
    14281 tataggctgg ggtattaata ccttttggac atactttaca ataaccacaa cctgtacata
    14341 tttcgttcat attcttattt aatctagtct caatatcatg tatatcctca tctgaataaa
    14401 ttctattttc gtctgcaatt ttacatgcat catctatatc ttgtttttta ttaaacccta
    14461 tcaaagatat acttatttgt gaaattccta tgttgaatct taaagctgat tctatagtac
    14521 tttcgccatc ttttgataga aaagaaagtt ctttattata ctttggaata gttccaccac
    14581 ttaatggatt cattgccaca acaccatatc cattatcaac agcatatttg gcacctttta
    14641 gcctatatgg aaagtttaat atatttattc ccatagttac accttcaaac ttattttcac
    14701 ttagaatatc tataacctca tctcctggtt gatgagatga aaatactata tggtctatca
    14761 atccttcttc tttacatttc aataatcctt catattgacc tccaggtctc attgctagtt
    14821 cgtaatgttc catctttcta atattccaaa catgataaaa atttatttta gatacgccta
    14881 atcgttctat tgattttttt acagcctcta tagctttttc tgatgtatca catgcttttg
    14941 gcttttcttt agttgataca taaaaatcgg tcttaccttc ctttatcatt tgtctaaagg
    15001 ctagtccaaa tatatcttca cttctgtcat cacaataccc tggtgctgta tcaaagtaat
    15061 taataccttt ttcatacgca tatttgacta aatcagcatt ttcttgatta cttttttcta
    15121 aatcaaacct taatcctcca tagccaacta ctgaaaccat tttccctgtc tttccatatt
    15181 caacgtatct cattgtaaat tcctccttag atatattaag acttaatttc tttatagaaa
    15241 atattatacc ataaataatt tttataaatt atttatggta taatattttt tttataaatt
    15301 atattttttt ataacttgta ttttataact aagttaaaaa ctataaaaag gttcaatatg
    15361 gactattact tgagcatttt cattaatttc ttcttgtatt tgtttctcaa ttttatgtgt
    15421 taaatcatga gattgctcta cgcttataaa tggatctacc attacatgca tgtcaatata
    15481 aatagaactt ttgctacctc tacttctgat attgtgaaca tcccttacct cattaaattc
    15541 aaacacaatt tcttttatat aatcctcatc tacagccact ctatcgacca aaattccaat
    15601 agatggccta aatattccat aagccgaata aattatgaat gcagaaataa taataggaac
    15661 tatagattct attattactg gaaatcctaa cttaacacca actaaagtaa ccaatacacc
    15721 tagagacaca aatatatcac ttcttgtatg caatgaatcc gaaatcaaaa cataactatt
    15781 tagtttagtc ccaattctat attcatacat gcatacaaaa atatttataa aaagtgtaat
    15841 aattaaagca attaaacttt ctatagttat agcaggtaca actggattag ctattcttaa
    15901 cactgcacta agaattatct taattgctat aacaaacaac ataccactta taaataaact
    15961 ggttataatt tcaaactttt tatgtccata aggatgattt ttatcttttg gtttagatgc
    16021 aaagaaaata cctataagcc ccactatatt agatgctcca tctgacaaag aatgaaatcc
    16081 gtctgctgtc atactatagc tttttatttg attaccaata atgattttta ataatgctac
    16141 tgcaaaatta gcaaataaaa ttatccacaa tacttgcttc actttcttat agttatccat
    16201 aacaaattcc ccctcccttc gattcaataa agaaagctat ctatcaatat caatcaacca
    16261 gacaataaaa tcgtatataa gcacatttac tatatttata ttcaaaattg gttgtacttg
    16321 ttcattctat ttgaaactta attataaata aaaatcgtag gtatggtttt ggtatacata
    16381 aaaataagag actgtctcaa aataaagata atcttctttt ttattgttga aatgataaaa
    16441 tatatttgat ttcatataaa aaagagtgcc tcatgaacta aaaagttcat tttgagacac
    16501 tcccttaaat ttttgatata aactattaat tttttatact aagccttctt taactaagaa
    16561 tgaatgagca acttcttctg gtgatttacc aagttcatcc acttgatagt taagttctct
    16621 catagtttct tcatttattt ttcctgaaag catattcata actttttcta actcaggata
    16681 tttcttcaaa gtatcttctc ttactaatgg tgctgcataa tatggtacaa agaaattttt
    16741 atcgtcctct aatatcttaa gattaaactt tttaataaga ccatcagtta aaaatgcatc
    16801 tataacctga gattcattat tatctaaagc tgtatatctt aaacttccat tcattgcttt
    16861 tacatctttg aattttaaac cataatctct agaaagccct acaagaccat cttctctatt
    16921 ttcaaactcc aaagttggtg agaatgtaaa ttctttacca tatttagtca aatcagaaat
    16981 agtatttatt ccatactttt cagcagtttc aggcatcata gctaatgtgt aagtattatt
    17041 aaatccaaga gggtcaagta aagttaactg attttctttt tccatagtat cttttacaac
    17101 attatatgct tcatcagcat ctttaatcgg agcatgtttc attacattta ccaaaagtgt
    17161 acctgtatag tcaacataca tgtctaactc accagattta attgcattaa acgcaacaga
    17221 tgaaccacct agatttaaat tcttcttaac tttaagattc gtattttttt ctactaattc
    17281 agcataaaca ttaccaagta taagttgttc tgtataatct ttagaaccaa tagttattgt
    17341 atctttttta cttgaaaaac tagaactaaa aacaacgaac aacattgaaa caacaaccac
    17401 tacagatatt actttaagtg ctatataatt cttcttaggt gcttttggat ttaatccttt
    17461 tggagtaact gcaacttcaa ttttactaaa taaataatca actaacagtg caattataca
    17521 tgatgggatt gcccctgcta aaatcatatt attatttaca gtttgaaccc ctgaaaatac
    17581 taaatatccc aaaccaccag cacctataaa tgctgcaaga gtcatcaaac caacagctgt
    17641 aactgcagat attctaactc cacccattat tataggaagc gctaaaggaa attgtatttt
    17701 aaataatgtc tgatttcttg taagaccaat acctttagct gattctaata ctactgggtc
    17761 aatacttgtt atacctatat acgtattttt tacaattggt agtaatgaat aaaccactac
    17821 cataaatatg gctggtttac ttcctatacc aagtataggt acaagaagac ccaaaagtgc
    17881 catagacgga actgcttgga ctaagtttac aaatccaagt acaaactttc taagagatga
    17941 gattcttgaa ataattattc caagaggaac accaactatt atagctatta aaatagatgt
    18001 aacagttaga ctcatatgtt gtattaaaag ctctataatc ttatcttttt gtaatagtat
    18061 aaagttaaaa aaattagcca agaacactca cctccatttc taagaattgt tcacttaaaa
    18121 cagaaagtaa actacttcta gtaataagcc ctaccagttt attttcatca gaaataaccg
    18181 gaatatatcc aaccgaatta cgattcatta catttagtat ttctactaaa ttatcacctt
    18241 catttacatg caaaggctca cttgacataa tatcagcaag taatattgac ttatcatcaa
    18301 tatctttcat atcttttaca gtaacaatac cttttaatgt ttttgctcta tctataatta
    18361 gtaagctatc aaccttactc gttctcataa tttctattcc ttgagttaca gttctagtag
    18421 aattaacagc aataggattt tttatcataa tatctcttgc ttttatatat tctggattat
    18481 cccaaactct atcttctccg ataaaatctt ttacaaagtc atttgcaggc tttctaagta
    18541 tattttcggg agtatcatat tgtgctattc ttccatcctt cataatacaa attttatcag
    18601 ctatctttaa agcctcatcc atatcatgag ttacaaatat tatagttttt ttcaattcat
    18661 cttgaagaga aaataattgt tcttgtaaag atgttctcgt tattgggtct aaagcactaa
    18721 aaggctcatc catcaatatt atttcagcat cagtagcaat ggctcttgca acaccgattc
    18781 tttgttgctg tccaccacta agttctgaag gatacttatt caaaaattca tcactatcaa
    18841 gtcctaccat ctcaagtagt tgcaacgttc ttttttcaat ttcttcaacg gatttttcct
    18901 tttttaggcg tggaattagc tctatatttt cttttattgt aagatgaggg aataggcctg
    18961 tgttctgtat aacatatcct atttttcttc taagctctat ttcattttcc tgagaaattg
    19021 gtttgccatt aatatatatc tcaccagatg taggttttat taatttgttt ataagcttca
    19081 aagtagtagt ctttccacaa ccacttgaac caatcaaaac taccaatgta cctgtttcta
    19141 caactagaga aatatcatct aatataacat tatttcctat tttttttgtt acatttctta
    19201 tctctatcat aatttatcat cccctttttc gaacaagcaa ccttcttcaa tattataagt
    19261 tgtttttata attatatcat actattttat ttatgtaaac aaagtacgat ttttaggtca
    19321 ctatttgtct gaatattgct acaaatataa agtatttata tatagtatat aatatataat
    19381 gtcaaaaaac aaaaaatata aaatataaaa atattcataa aataacacta gcctcaatta
    19441 tatagtatta aagctagtgt tatttcattt aaacaaaact cacatttgga tactatccat
    19501 gtattcaaat aatgtaatat ctttataagt ttctttttta tcacttaggt ttgatattgt
    19561 aactcccaat agcctaattt gttttccatt aacctcaaca tttctcaaca aattatctac
    19621 accacttctt atatcattat attcgtcaat atagtgttct aaactaaggc ttctagttat
    19681 ctttgtaaag tcctcatatt taatttttaa tgttaatgtt tttccaaact tctcagagtt
    19741 ttttaatcta tgacatactt cttcacaaag ttcatcaaga atgttgcgtg tttcttcttc
    19801 atcaatatcc aaattatgac ttaatgtagt ctcagcccca acagattttc taaccctatt
    19861 tggttccact ggtctatcgt ctattcctct agcaaattta tataattcat aacctctttt
    19921 tttgaatata ttttcaagtt caaataaatt taaacacctt aaatcatatc cagtttttat
    19981 acctaaattt ttaagagtat ttgaagtaac ttttccaaca ccaaaaaatt tattcacagg
    20041 taatttatct aaaaagtcct gggcattttc ttcggttatt acagttaatc catttggctt
    20101 tcttaagtct gatgcaatct tagcaagaaa cttattgtat gacacaccag ctgaagaagt
    20161 aagaccaact tctctaaata tatctttttt tatttgcttt gctatctcaa ttgatgaatc
    20221 aatatttttt ttattttttg ttacatctaa gtaagcttca tctaaagata atggttcaac
    20281 taaatctgtg tatctataaa atatatctct tatttgctct gacacactct tataaacatc
    20341 aaatctaggt cttacaaaaa ctgcatatgg acacctattg taagctacag ctgatggcat
    20401 agctgaatgg ataccaaatt ttctagcttc ataagaacac gttgcaacca cacctcggct
    20461 ttgtggattt ccaccgacaa taacaggtct tccttttaat tttttattat ctctttgttc
    20521 tatagatgca taaaaagcgt ccatatctat atgaattatt tttctataat gtttcattat
    20581 tatcaccttt taaagtataa atagattaat tttatttata ttacttttta attataccaa
    20641 acttatgttt ctttttggta ttttttcatt attttcttta tatattcata ttttccataa
    20701 ataaaaccat ctataatcca ttaacttcaa tataatttat aaaaaatcct taaaaacact
    20761 tgcaaaaact atataaaagc tttacaatag gtattccaag gaactaaatt taaaattcct
    20821 tatggataac agtatatata tttaatttat gggatgtttt aaaaacaaaa gactgtctta
    20881 tacaagacag cctactgatt ttattataat tgtaatggac tgctcaaact attactttaa
    20941 aacagcccat atttttttat ataattttaa ctatagtaaa gattttgtga aggatattct
    21001 gggtcacaag ttacatctat accaagtttt ctaaatgttt gttcatcatc tctatttaat
    21061 atagtagtag aatgagcttg acaccctttt aataatgcta acttttccat agcaaattgt
    21121 gcagttggat tagttacagc acaaatacta agtgcagtta acacctcttg gcaactaagt
    21181 gaaacatttc tactagctaa agtctttgtt tttaagttta tgataggttc taatattaca
    21241 ggtgatatta agtgcatatc atcagatata ttggctaaat gtttaatggc atttaatact
    21301 actgctgcgg ttccattcat taaatcagaa cccttaccag ttaatattgt accatcttct
    21361 aattctaatg caactacagg acaagtatca ttcttgtcgg cactttcttt caatttatta
    21421 ctgtagtttc tagcaggtaa tacaacattt ctatcttctg gctttaagtt aagctcttcc
    21481 ataatcattt tcattctatc ataagcacct ttatcaactt gtcccttttt atactcacag
    21541 gcagttttaa agtatcttct tattatttct tctatagaag cttttcttat tacttcatca
    21601 tctattattc caaagccaac tctattaaca cccatatcag ttggagattg gaaaattgat
    21661 tcttttccag ttatcttttc tattattttt ttaagcactg gaaataattc caaatctcta
    21721 ttgtagttta ctgacatttc accatacgtt tcaaggtgga atgaatctat catattcaca
    21781 tcttttaaat caactgtagc tgcttcataa gctatattca aaggatgttt tacaggtaca
    21841 ttccaaactg ggaatgtctc aaacttagaa tatccaacta cattacctct tttattttca
    21901 tgatacaact gacttagaca agtaccaagt tttccactat taggtcctgg tgctgtgact
    21961 actactattg gctttgtagt ttctatatat tcatttgctc catatccctc atcactaact
    22021 atagtctcaa catctgacgg atatcccttt gttggataat gcttataaac tttaattcct
    22081 ctacgttcta atttatttat aaatacagtt gtagcaggtt gtccttcaaa tctagtaatt
    22141 actacactat ttacatctag ttcataacct cttaaatcat ctattaatct taaaacttcc
    22201 atatcataag tgataccaaa gtctcctctt attttgtttc tttctatatc tcctgcatag
    22261 acacaaatta caacttcaac tttttctttc atatgttgta atacttttat ctttgcattt
    22321 tcatcaaatc ctggaagtac tctttttgca tgaaggtctc ccatcaattt tcctccaaac
    22381 tctaaataaa gtttgtcaaa attatttact ctttctaaga tatatttaga ctgctcttct
    22441 aaatattttt tgtggtcaaa tcctactttc atcttaacac ctcactaaat taaatttctt
    22501 attgataaca gtatatttat atatattttt ttagtaaaac atcttataaa aaaatgttaa
    22561 tactttttca ctcaaagtaa tattatacaa ttaagattaa tttttttcaa gatagtttta
    22621 tggtaaataa gatataccta atataactaa taaaataaga atactatatt tttatatata
    22681 agtataatat tctcacctta gcattaattt atttcaatat tttcatatat cttttattct
    22741 aaattcaata cttttctaat atcttctata ccttgataaa cttcttgttt agaaaaattc
    22801 atagcttcta attcttcatc agttttttcc ataagcatag tataaaattc tattgctata
    22861 tactgcatat tttcactatt tacactctta gctttatcaa ataaaatttc ttctgcttcg
    22921 tttacatttc cctgaaaaac taatcttttt aaagttataa gtataacttc ttcttctgta
    22981 ttatacacta aatctttttc ttcatattca ctgtattttt ttcccaataa aagtcttgca
    23041 accataagtt ttatctcatt gaaaatctta tccactatag ccatttttac atctcctata
    23101 aataaatttt attttctttt taattatatc agactttatc gtaaaaatta ttatatgtaa
    23161 atgaaataat tcaaaaataa attttaacaa taaaaaaaag aaggtagcgg gggatttccg
    23221 actaccttca aacactagat tgtgattggt ttggtatttt atttataccc accataagct
    23281 ttattaaatc atctaaattg atttttttag catccacatc ctccatcaaa aacgtcacca
    23341 cattcacaaa gtattaataa taatattaag aaaggtatcc atgcacataa gtccatttca
    23401 gcccaacaat cttggaatgc taagaataag aagaataata ttattatcca ccaagcacat
    23461 ccaccaaatc cgccttcacc aaaaaatcta tccaacataa tatgttacct cctagtcatt
    23521 ttgtatgtgg agtttcagct cctaaaatat actatgttta aggggtaata tttgctaatg
    23581 atttttttaa atttttttag atatttttaa aaaaattatt gattcacact caatgtctta
    23641 ctatcactat tgataactac ctctccatta gaagtcaaaa atgtttttat attcaatctt
    23701 tccaatattt ttaaaacacc ttcctctggt tcttttttct ctgaacatgt aataacagca
    23761 tattggggtg aaatagactc taaaaatgtt tcagttaatt tgtcatatct tccatgatga
    23821 ggcattttaa caaaatcata ttttaaagta tttccattta taaactctga caatctttct
    23881 tcctctgcat ctcctgcaaa taaaaactta tgtgctccat agcttatatt agtgattatt
    23941 gaataatcat ttgaactctc gtacttgctt tttgaagccg gatgtatgtt tatttctgca
    24001 ccattgatta caatattcat attatctttt agtagcactg gttctatatc agctttttta
    24061 agtgagtcaa tatattcatc atattgctta ctatctttct tataatttgc ttgtataata
    24121 ttctttactt taacactaga taacacacta tctactccac ctatatggtc cttatctaaa
    24181 tgagttaaaa ttaaatagtc aagtgtattt attccttttt cttttaacct atccactatt
    24241 tgttttccat ttttatcttc tcctgtatca atcattatga ctttgttacc tatctcaacc
    24301 aatatgcaat cagactttcc agtgtttagt atagtagtct taaatgcagt attttttgtg
    24361 ctactctttg tgctcctctg gataccattt tgcctcacac tatttttgtc actttttaaa
    24421 taagattcac ttgttgattt tatcttacta ttagtagtta tatttgttac aagcaataat
    24481 attactatta tagataatgt agtaactaaa aacaggatat cttttaatac tattttcctc
    24541 atccaaaagc tcctataaat tttatttatt acatatttat agtatagcaa ccttaactct
    24601 gtaaaataca catataaaaa gggcaaattt taggcatatt tgtggcatga taaaaacatg
    24661 ataaaaaaat gactgtacca aaacaattat ctttctaaat ttacatcttc aaattcttta
    24721 atcttatcat tagataaaac actcttttta gtagattgtc cattttttct atcaaaagta
    24781 tttttagctt ttacacctga taaaaactta cctgctccat taatacaacc ttcttcacac
    24841 atcattcctt ctataaaatt accttctaat cttcccaatt tagccattgt cattgtcttt
    24901 ttaatttgtc ttggaccaga tacttttata ggttcaaaat caaaactaag ccccttttca
    24961 gcaatatagt tctgtactga tttggcaagt ccaccagatg ctccaaatcc ccttccaaaa
    25021 atcgaaccat catttatatc ttcatttgaa cattttgata aatctatatt aaatgcttca
    25081 aataatgcca ttagttcttc aaaagtaagt acataatcta ctgaatcttt taaacttttc
    25141 tctgttgcct cactcttttt agctgtacat ggacctataa atacaacttt agcatcagaa
    25201 tctatttttt ttactaatct tcctgttgct accataggag acactgtaga tgatattcta
    25261 tctttttcat ctgggaacat cttctctata tacttaaaaa atcccggaca acatgaattt
    25321 gtcatgtaat tatctcctga ttttattctt tcaataagtt cattgctttc atgaactgta
    25381 actgcatctg ctccacaaga agcttctatc atatctacaa atcccagtga ttttatagca
    25441 ttttttaact gaccatatgt aatattacta tctatttgac ctgttatagc tggagctact
    25501 attgcataca tattttcttt ctttgcaagt ttttttgcaa caggtgctat caaactctta
    25561 tcagatattg ctccaaaagg acatgctgac atacatgctc cacaatttat acaattatct
    25621 tcatgtatca ttgctttcat agtgtttcta ttaaaatcca aagcccctgt tggacaaacg
    25681 cttttacaag gtcttacaac ttctgatata gcatcatatt gacatgaact tttgcacatt
    25741 ccacaagcct tacataaatc atgatttata tatgctctcc catttatata agaaattgcc
    25801 ccaaatttac atgcttcctt acatctatga gccaaacacc ctctacaaag ctctgtaact
    25861 gtaaattcat ttattggaca tctatcacat gctgcttcaa ctatatatat aatttgctca
    25921 tctgtgtcca aactaactga atctggattt atatctccat atttactatt tggagttaaa
    25981 cttaatgcaa gttgcgccct ttcaagaact acttctctct ctttataaac acaacatcta
    26041 tatctcgcct tttctccttt aattatttca tatggaattt tttctatatt ttcttttgtt
    26101 aaattttctt caatagatag tcttgctact gctgttaaaa cttcatgttt taaccttttt
    26161 agttgcgttt caaattcaaa cataagtaat cctccctata aatcacacct ttttgctata
    26221 tttctactat tatgtatatt gtatttttta tttatttcta taaattatta taacataggc
    26281 atatactttg ttaagttata tacaaaatat tatatatgta atttttattt taagtgtata
    26341 taatttttat tttagttgtt caataattaa atatataaat aaaaatagag ttgtccaaaa
    26401 gttaaacctc aacttagaac aactctatta acaatatgta tttttgttgc tttgtattat
    26461 ctttattttt ctaaatttat attactaaaa tcttttatct ttgtatttgc taagacactt
    26521 ttcttagttg attgagaatt ttttctatca aaagtggctt taacctttgc acctgataag
    26581 aatttaccag aaccatttat acaaccacct tcacacatca ttccttctat aaaattacct
    26641 tctagctttc ctaaattagc catagtcata gttttcttga tttctcttga accagaaatc
    26701 tttacaggtt caaaattaac accaatacct ttttcagcaa gataattttc tatagctttt
    26761 gtaagaccac cagcagctcc aaaacctctt ccaaaaattg atgcatcatc tatatcttca
    26821 tttgaacact tagccaagtc tatatcaaat gcctcaaata atgccattat ttcttcaaaa
    26881 gtaagtacat aatcaacagc tccttttaat tctttcttag ctgcctcact ctttttggct
    26941 gtacatggtc ctatgaatac aactttagca tctggttcat gactttttat aaatctacca
    27001 ctagctatca taggagatac tgtagaagat attttcccta catgatttgg gtatgttttt
    27061 tctatataac caacaaatcc tggacaacaa gaatttgtca taaaagcttc tcccttttcc
    27121 attctttcag caaattctaa gctttcatga actgtaactg catctgctcc acaagctgct
    27181 tctaatacat caacaaatcc tagtgatttt atagcatttt ttatttgacc atatgtaaca
    27241 tttgcttcaa cttgacctgt tatagctgga gctaccaccg catacatttt ctctttttga
    27301 actaactttc tagctacagg agctatcaaa ctcttatcag atattgctcc aaatggacat
    27361 gctgacatac atgctccaca gtttaaacat ttatcctcat gtatcatagc tttcatattt
    27421 tctctatcaa atcctaaggc attagttgga caaacactct tacatggtct aacaacctct
    27481 gagatggcat catattgaca agctttttta cacattccac aagccttaca taattcatga
    27541 tttatatagg ccattcctcc gacatgtgtt atagctccaa atttacaagc ttctttacat
    27601 ctatgtgcta gacatcctct acaaatatct gttactgtaa attcatttat tggacaactg
    27661 tcacatgcag cttccaaaat atatataatt tgttcatctt cttctatatc cattgtatct
    27721 aaatctatct tatcttgacc tggttgtaac cccatactta catttactct ctcagatatt
    27781 atagctcttt ctttataaac acagcatctg tatgtaggtt tctcaccttt tattatttca
    27841 tactgaatat tatccattgc ttctctggta aggttatctt cttttccaag tctagctatt
    27901 gcagttaaaa catcatgtct taatttgtgt atttgtgttt caaattgaaa cattttaata
    27961 ccacccccgt aaatgatttt atacgatacc attataacac aatatgtcct tttattaata
    28021 tatacataaa atattatatg tttaaatttt attttatcga atatatatag tgtttttttg
    28081 ttttattata taatattatt gatatttctg aaatttcact atttttattt ttacttttca
    28141 atctaatata taatattttt tattatatat tttaatttta ctttttactt acataccttt
    28201 ttgttacatt ttttgatatt aaatttatta tctaca
//
LOCUS       DAGSRM010000040        28174 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.040, whole genome shotgun sequence.
ACCESSION   DAGSRM010000040 DAGSRM010000000
VERSION     DAGSRM010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28174
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.040"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(185..361)
                     /locus_tag="MJE33_001974"
     CDS             complement(185..361)
                     /locus_tag="MJE33_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647908.1"
                     /translation="MFRDKMDRCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(415..511)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1307..1708
                     /locus_tag="MJE33_001975"
     CDS             1307..1708
                     /locus_tag="MJE33_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HBY9647909.1"
                     /translation="MNYKNRKRAYYQENSCMDNFICKNCGCLVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNS
                     VKLMSIALKPLAQPPFPLERIIEKLEKEETE"
     gene            1705..2772
                     /gene="rsgA"
                     /locus_tag="MJE33_001976"
     CDS             1705..2772
                     /gene="rsgA"
                     /locus_tag="MJE33_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBY9647910.1"
                     /translation="MNGMKQINLQDYGLNRNFITQSMEYYDLYLARIISQEKNFYQAV
                     CVNGVLFAEVSGKLRFEATIQSDFPTVGDFVMLDRCNNKNGNAIIHHVLHRKSLFVRK
                     AAGKVNEEQVVAANIDIVFLCMSLNKDFNIHRLERYLSLTWESGAIPVVVLTKSDLCD
                     NVHRKLQVVNSIALGVDVVVTSSIEPDGYKQISKYIKPGCTVALLGSSGVGKSSLINC
                     LMGGEFLNTKEIRIDDDKGRHATTRRELFLISGGGIVIDTPGMRELGMWDVSEGLDKS
                     FADIEAYFGKCHFRNCTHTSEPGCAIWEAIAHQELSPKRWESYRRLKAEAVFADNKSE
                     YLAKKEKKFKNISKINKTIRR"
     gene            3308..3994
                     /locus_tag="MJE33_001977"
     CDS             3308..3994
                     /locus_tag="MJE33_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647911.1"
                     /translation="MKISSQYRSQYSFRYESNINNTRINESMVKKNETVGKDTYLSNI
                     MKQKQELNDRIRDLKYRQEVYTKKINDAIKNLCKSEIRETTNNFSNIEIGIKNSIIED
                     KNKSTMLDENSTYLNTNDEKESLITKESNEKIEEEILNDEKLEELEQKKDSNKKEKVS
                     EDLSLVGKTREELENMLKNFINLTQEEIMKLESRIEKLDKNAEEYKQNSKTNIFDKTD
                     EQKKHINVLI"
     gene            4172..5062
                     /locus_tag="MJE33_001978"
     CDS             4172..5062
                     /locus_tag="MJE33_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647912.1"
                     /translation="MKKNILTTVITMIIILMMSMTVMAEPYQEDETSSLKKQSANERN
                     QTNIGTMDGRVQTNAISKGDNYDPKYPLKGMLTQLGLNTTGKEGWDLLYGGNPNGVEL
                     RADGKYYYINKGMEGLGFYAAALSGGSYQRIGFGKLDPIEVIADGNWISYKQAQDTLT
                     VIRNFLNSFDWRNASEMERANRAAKLVTEAKYADSKYCNIVYGNLVDKRGVCGSFASS
                     FHLLTRLMGMDSLSILNPSLNHAWNYIQIDGKWYRFDGSEISAFGGSLDFDYRKLKDA
                     TREMTTYYDAKALSILGFNQ"
     gene            complement(5464..6420)
                     /locus_tag="MJE33_001979"
     CDS             complement(5464..6420)
                     /locus_tag="MJE33_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBY9647913.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISLLDDNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRVTDDMIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKKMLKTVSHGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIHFSNILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENNSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
     gene            7124..8119
                     /locus_tag="MJE33_001980"
     CDS             7124..8119
                     /locus_tag="MJE33_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBY9647914.1"
                     /translation="MSIIELNELTKSYRRFEKKAGFMGSVKSLFNRKYTEKTAISSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEKLFNYFQKYKKITVSFSNETNIVFPKDCKVVEQT
                     NHKTSVIVPKERGNETIKWIFDKYDVKDVMIEEEDIGSIVERIYKKGSDEYYEQL"
     gene            8097..8960
                     /locus_tag="MJE33_001981"
     CDS             8097..8960
                     /locus_tag="MJE33_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBY9647915.1"
                     /translation="MSIMSNYNEASSNYSMFYKFKQSIRRYYEVAKINFKVQLIWRFD
                     VVVNMVFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGIDMSKSTSE
                     EICNRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFT
                     SNPVQILISILMIFLGFIFMMKLHFFIGILSFKFLEISFFRMIIENIVEFITGTMIPL
                     VLLPETIVTVMKVFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYK
                     RMRTLFDGVGI"
     gene            8957..9751
                     /locus_tag="MJE33_001982"
     CDS             8957..9751
                     /locus_tag="MJE33_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBY9647916.1"
                     /translation="MMKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLM
                     FNSIYSHVDSIGGWKQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYIT
                     KPLDPLFRLTFENINIGSSPLILASILIVLYGISELNSEITLAMIIIYIFMVLLMTLL
                     FYDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGVFKVLFYLILPYGIMS
                     TIPTQILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            10036..11415
                     /locus_tag="MJE33_001983"
     CDS             10036..11415
                     /locus_tag="MJE33_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBY9647917.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILVFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTVSGMVFFSKNKGLLKFCIPRLDKRILFEACFNGSSEMVSQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTLFLTIFCIC
                     KYKNRYKYM"
     gene            11863..12576
                     /locus_tag="MJE33_001984"
     CDS             11863..12576
                     /locus_tag="MJE33_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY9647918.1"
                     /translation="MDTCFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIAFRVKAQLRRQQYQSIVPSDSEVIKIGDITIDIEGNRVYKDRNEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(12848..14386)
                     /locus_tag="MJE33_001985"
     CDS             complement(12848..14386)
                     /locus_tag="MJE33_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBY9647919.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGTGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINRDTTISLYLHTAFEELYKIEKEMN
                     LLYQKSAISENEQYLIKASDYQEQLIASNFYSIDNEINKIANGLGLDSIGMNRVVREL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSNYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEETFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
     gene            15268..15723
                     /locus_tag="MJE33_001986"
     CDS             15268..15723
                     /locus_tag="MJE33_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBY9647920.1"
                     /translation="MDEIDLKIINSLKKNSRASTSEISRQINLSVPAVSERIRKIEQS
                     NLIQKYTIQINREKTKYKLLAFIFINTDLSKSMKEFKKTILEYDTVLECHHISGEYDY
                     LIKVLAEDTSNLEEFISVTLKEIGGIQKANTTIVLSTIKEEFNTIKDIF"
     gene            15772..16356
                     /locus_tag="MJE33_001987"
     CDS             15772..16356
                     /locus_tag="MJE33_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HBY9647921.1"
                     /translation="MKVLVFLAKGFETMEFSVFVDVMGWARNDYGHDIDVVTCGFKKQ
                     VMSTFNIQVLVDKTIEEVCVDDYDALAIPGGFEEFGFYDEAYDSSFLNLIREFNSKEK
                     IIASICVAALPVGKSGVLKNRKATTYHLKNGKRQRQLSEFDVNVVNEPIVVDKNIITS
                     YCPETAPHVAFKLLEMLTSKEQMDEVKLAMGFKL"
     gene            complement(16361..16684)
                     /locus_tag="MJE33_001988"
     CDS             complement(16361..16684)
                     /locus_tag="MJE33_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY9647922.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLENL"
     gene            16836..17354
                     /locus_tag="MJE33_001989"
     CDS             16836..17354
                     /locus_tag="MJE33_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBY9647923.1"
                     /translation="MNFVELAKKRYSCRNYQDRKVEKEKLEKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGINKLSKAANIYDAPLAILVCGDKDKVWTRPFDGKQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKIPESPERHEKTR
                     VPLSEIVSYETL"
     gene            complement(17530..18198)
                     /locus_tag="MJE33_001990"
     CDS             complement(17530..18198)
                     /locus_tag="MJE33_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HBY9647924.1"
                     /translation="MLEVSHKVKEIYPNIKFGVMVINITHSKPNKENFLVLKNSTIKN
                     IIEQHPEYNRKEKIKTEPVSSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVGVTIES
                     MFLAELKNLLLTAGHDLDKMELPLKIELANGSEHFYGIGGKEHILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKHYISSAFPDLKVNS
                     IDIF"
     gene            18313..19728
                     /locus_tag="MJE33_001991"
     CDS             18313..19728
                     /locus_tag="MJE33_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBY9647925.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILDGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHKVDFKLNDTFRDEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKELSKLYKQILYDLSDSDFRYGSTAGVWVLRVEISKYL
                     FRTRGIRCNPKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGIKVDDKGMNTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKCATENDYYII
                     EDDYDSEFRYEGQPISSLYELSPNKVIYIGSFSKILSPAIRVGFMLLPDNILESYKTL
                     KMYSDVHTETILQHVLAEFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQYAIENILEYDYEIILGYAHLS
                     FDEIEKGVKILSKIIHMNQGT"
     gene            19951..20409
                     /locus_tag="MJE33_001992"
     CDS             19951..20409
                     /locus_tag="MJE33_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY9647926.1"
                     /translation="MEIIDYIEENLTENLNLDKIAYIANYSKYHLHRMFTNVVGCTIH
                     QYIQRRRLTEAARQLIFTDKYIIDIAITVGYSTHQSFTLAFRNLYKESPQVYRNKDEF
                     YPIQQRFVLNLNHQIQSEKCISYANMNYKQRRDEINSYLNFNINCEVKVA"
     gene            20406..20990
                     /gene="clpP"
                     /locus_tag="MJE33_001993"
     CDS             20406..20990
                     /gene="clpP"
                     /locus_tag="MJE33_001993"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBY9647927.1"
                     /translation="MSLVPHVIEQTGQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSATSGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRCALPNSEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDKILSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
     gene            21302..22378
                     /locus_tag="MJE33_001994"
     CDS             21302..22378
                     /locus_tag="MJE33_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBY9647928.1"
                     /translation="MITISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGNPTLSYYRERLFKRSSNHKWVDPIHEVVPLFGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKTLEPRHQFYYSRELYYNARYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTNGGFKSNDCYGYIPSIQLSVCYDRLGESDKAIYYHEK
                     TKEIKPNDSAVLHNENYFSKFKNS"
     gene            22445..24511
                     /gene="bclA3"
                     /locus_tag="MJE33_001995"
     CDS             22445..24511
                     /gene="bclA3"
                     /locus_tag="MJE33_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA3"
                     /protein_id="HBY9647929.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNNGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGATGPTGPTGPTGPTGNTGNTGATGLR
                     GPTGATGATGPTGAIGFGVTGPTGPTGATGATGADGVTGPTGPTGATGADGITGPTGA
                     TGATGFGVTGPTGPTGATGVGVTGATGLIGPTGATGTPGATGPTGAVGATGIGITGPT
                     GPTGATGADGATGVTGPTGATGADGVTGPTGATGATGFGVTGPTGPTGATGIGITGAT
                     GLIGPTGATGATGATGPTGVTGATGAAGLIGPTGATGATGADGATGATGATGATGPTG
                     ADGLVGPTGATGATGADGLVGPTGPTGATGVGITGATGATGATGPTGADGLVGPTGAT
                     GATGADGVAGPTGATGATGNTGADGATGPTGATGPTGADGLVGPTGATGATGLAGATG
                     ATGPIGATGPTGADGATGATGATGPTGPTGADGLVGPTGATGATGATGATGPTGATGA
                     TGATGPTGASAIIPFASGIPLSLTTIAGGLVGTPGFVGFGSSAPGLSIVGGVIDLTNA
                     AGTLTNFAFSMPRAGTITSISAYFSTTAALSLVGSTVTITATLYQSTAPNNSFTAVPG
                     ATVTLAPPLTGILSVGSISSGIVTGLNIAATAETRFLLVFTATASGLSLVNTVAGYAS
                     AGIAIN"
     gene            24680..25441
                     /locus_tag="MJE33_001996"
     CDS             24680..25441
                     /locus_tag="MJE33_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HBY9647930.1"
                     /translation="MKNKIEISMNNFVKNYHKSNNITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHAMPKDFLENGKTIITYFIPFDESVNNSNIGGFECSEIWARAYIETNMLIS
                     NLNDFLCEELEKMGANSYAIPGSYNFDSEKLVSDWSQRHVAFIAGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDGFNRNKCY
                     EMCMINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            25750..26028
                     /locus_tag="MJE33_001997"
     CDS             25750..26028
                     /locus_tag="MJE33_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647931.1"
                     /translation="MSYKKAIHILPEELLELIQEYVDGECIYIPRKSNNKKEWGSNTP
                     TREELSIRNMQIYEDYQVGYDLQYLSEKYYLSLKSIQRIVLQEKRKDI"
     gene            26321..26896
                     /locus_tag="MJE33_001998"
     CDS             26321..26896
                     /locus_tag="MJE33_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HBY9647932.1"
                     /translation="MKKITWEVEYISQLPVIRYCKKCGKKTEYICSGLFRVNAQHKYL
                     DIWLIYKCSNCDSTWNSTIYSRVNPNRLSTETLEQFHTNDDNLANQYAMNVELLHRNG
                     AEVGIPEYKIVGQEIDINDLTQVRIVSKYPSQLKVSALLREKLGLSRKAFDEMLDCGT
                     IKSISGVDLRRSKLNYEIVLEVGKQKQKSSN"
     gene            27335..28045
                     /locus_tag="MJE33_001999"
     CDS             27335..28045
                     /locus_tag="MJE33_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY9647933.1"
                     /translation="MINIGICDDELHYRIKIKDILSEILSSYPINYNIYEFSAGEELL
                     NNYPKDLDILIMDIQMKTINGMDTARKIREFDHKLEIIFVTSFVEFMQEGYEVKAYRY
                     ILKPINKEKISKSVLPCINEMMKKRNNYLTINVKNYVDRIKIDSITYIETDRPNILIY
                     THDDMYITKMSISKIEKILNEYGFFRCHNSYIVNLKLVESMSGSTVIVDGKSIPISKY
                     RVKGLKLAITNILGDIVC"
BASE COUNT        10538 a         3336 c         4720 g         9580 t
ORIGIN      
        1 cacatagata agagaattta agttctcata tttattattt gatacaaatt tacatcttta
       61 ttatatgtat tttaaataaa aaataaaatt atgaatacta gaaatctatc ttaattagct
      121 ttctaacata aggctattta aagtagataa aactaaaaag ggttctctag aaaaccaaat
      181 ctatttaatt atacttactt actaatacca tcacttgatg caaacaagat tctaccacat
      241 tttctccgta ctctagtata aaatcatcta gttgtgtatc tataaaatca caataatcat
      301 atgaactacc aatataagca gtcaacatat gtgtacatct atccatttta tctctaaaca
      361 tatatattat tatcccccta aaaaactttc ataaaaacaa aaagaatatc tctttttggc
      421 aactggctga cataactcat agtttatttt ataagcgtta gtccctatag ctttgcgtca
      481 ctaaatttct ctagttttgc cgatttagtt ttattctaca attacaataa tacaatagaa
      541 ttgcttatta ttcaatatac ttatttgaaa attaaataaa tttacaaata tgaacgttat
      601 tttatatcaa aaaaatagac tatgcatgta gaatatatgg aaaaacttga ttgtaggttt
      661 tgtatgaaaa gacagttgtt attaatttaa gtgagataaa ttgagcaata ggcataaaat
      721 gagaaaacaa ataatagcag ttaattttta ttattaatac taggtgaaca ttaaatttta
      781 aaataccact agattttata gaataagaaa atgagacaaa cataaatata tgaatatatt
      841 aaattatact tgaacatact tatattttat gataaaattt caataataat tattagtgaa
      901 ggagtgtctt gcctgatgtc agagttatat ataatggtaa ccaacaaata aatagttgga
      961 gacgcatatg ggttctgttt aatcagaatt tttaagtgta tttttgatac atttgattgt
     1021 tgtgtcttac tctgccttta tagataacta acattagaca acaaataata atattacata
     1081 ttactttgtt aaattgtggt gtagtgggat ttaatctgct acacttttat taattttaca
     1141 cagtattatt ttgaatataa ttatgttgaa atagaagata ttaaattttc tagtccacag
     1201 gggttttctg tttttgtgta ggtaaatatg tataccacat aaaaaggcag atataaatac
     1261 ctgtggactt tttttgtaga aaatacaaga tacggaggaa ataaaaatga attataaaaa
     1321 tagaaaacga gcttattatc aggaaaattc ctgtatggat aactttattt gcaaaaattg
     1381 tggttgcctt gtagttcctg aaggagctgg tacacaacat agaaatcatt gcccacattg
     1441 tttacatagt cttcatgtag atatcattcc tggtgaccgt gctgcagatt gtgatgctaa
     1501 tatggaacct attggagtgt gggtgagaaa aaatggtgag tgggcaatta ttcatcgttg
     1561 tacacgctgt ggacatctta gttctaatcg tattgcagct gatgataatt cagtgaaatt
     1621 aatgtcaatt gcattaaaac cactggcaca gccccctttc ccactagaaa gaatcattga
     1681 aaagttggaa aaggaggaaa cagaatgaat ggtatgaaac aaatcaattt acaagactat
     1741 ggtctaaata ggaattttat tactcaaagt atggaatatt atgatttata ccttgctcgg
     1801 attatctcgc aggaaaagaa cttttatcaa gctgtatgtg taaatggtgt actgtttgca
     1861 gaggtttcag gaaagctgcg ttttgaggct actatacaat ctgattttcc cacagttggt
     1921 gatttcgtta tgttggatcg ttgcaataat aaaaatggta atgctattat tcatcatgtg
     1981 cttcatagaa aaagcttgtt tgtgcgtaag gctgctggaa aggtaaatga ggagcaggta
     2041 gttgcagcta atattgacat tgtttttttg tgcatgtcac tgaataagga ttttaacatt
     2101 catagattgg agaggtatct gtctttaaca tgggaaagtg gagctatccc agtagtagta
     2161 cttactaaat cagacttatg tgacaatgtg catcgtaaat tacaagtagt taattctata
     2221 gccttgggtg tagatgtagt tgttacctca tctatagagc cagatggtta taaacagatt
     2281 tctaagtata ttaaaccagg ctgtactgtt gccttacttg gttcttcagg agttggaaaa
     2341 tcttcattaa ttaattgtct gatgggtgga gaatttttaa atacaaagga aattcgcata
     2401 gatgatgata aaggaagaca tgccacgact agacgagagc tatttttaat ttcaggtggt
     2461 ggtattgtta ttgacacacc aggtatgaga gagttgggaa tgtgggatgt ttcagaaggt
     2521 ttagataaaa gttttgcaga tatagaagct tatttcggaa aatgtcattt tcgtaattgt
     2581 actcacacct cagaaccagg ctgtgctatc tgggaagcta ttgctcatca agaactttcg
     2641 cctaaaagat gggagtccta tcgtaggctt aaagctgaag cagtatttgc agacaataaa
     2701 agcgaatact tagcaaaaaa agaaaagaaa tttaaaaata tctcaaaaat aaataagacg
     2761 attagaagat aagtgtagtt tttttgttca ttctatgagt ctatatacta ttaatatatt
     2821 aaatttgcca tcaatttgtt actttaatgt gattttgatg gcaaatttgg tttgtggtat
     2881 tatttaaagc aatttattaa tttttttaat aaagtttgat tttttatatg atgatatata
     2941 catgtgataa tgtatacata tttgttacga taggctcctt tataaagagg tcttatgtaa
     3001 gataagaaaa ggcatattta tactaataca taatagagta tatgcttatg tatgatagag
     3061 atgtttctta agagcaaaac tgcaatattg ctaatttata aagtaaaaat atatgaatta
     3121 taaaatacaa ataatatatt tataaaaatt attgttttaa tttataataa aaattttttg
     3181 ttgaaaataa tgctcgaacc tataaaacta ttaactaata tttgtaaatg tatacttttg
     3241 atgataaaat ataattaagt taagttggtt tttataaaca taaatttatt ttaaggaggt
     3301 gaaataaatg aaaatatcta gtcaatatag aagtcaatat tcatttagat atgaaagtaa
     3361 tataaataat acaagaataa atgaaagtat ggttaagaaa aatgaaactg taggaaaaga
     3421 cacttattta tctaatatta tgaaacaaaa gcaagaactt aatgatagaa ttagagattt
     3481 aaaatataga caagaggttt atactaaaaa aataaatgac gcaattaaga acttatgtaa
     3541 atcagaaata agagaaacaa ctaataactt ttctaatata gaaataggta ttaaaaatag
     3601 cattatagaa gataaaaata aaagtacaat gttagatgaa aattcaactt atctaaatac
     3661 aaatgatgaa aaagaatctt taatcactaa agagtctaat gaaaaaattg aagaagaaat
     3721 attaaatgat gaaaaattag aagagttaga acaaaaaaag gattctaata aaaaagagaa
     3781 agtatctgag gacttatctt tagtaggtaa aactcgtgaa gagcttgaaa atatgcttaa
     3841 aaattttata aatttaacac aagaagaaat aatgaaactt gagtcgagaa tagaaaagtt
     3901 agataaaaat gctgaagaat acaaacaaaa ttcaaagact aatatatttg ataaaacaga
     3961 tgaacaaaaa aaacatataa atgtactgat ttaaacaata tgttttaaaa aaataagttt
     4021 attatataat gaaaatataa aggtacaatt tcaatacact tctattaaac caataattta
     4081 tgaatcagac ttaatttcaa tttactaata accatatatt tatgagttaa taattttatg
     4141 ttttaaaaag aattaaataa gggggagtag tatgaagaag aatatattaa ctactgtgat
     4201 taccatgatt attatattga tgatgagtat gacggtaatg gcagagccat atcaagaaga
     4261 tgaaacatct agcttgaaaa aacagagtgc aaatgaacgt aatcagacta atataggcac
     4321 aatggatggc agagtgcaaa caaatgcaat aagtaaaggt gataattatg accctaaata
     4381 tcctcttaaa ggaatgttaa cacaacttgg tcttaataca acaggtaaag agggatggga
     4441 tttactctat ggaggaaatc ccaatggagt tgagcttcga gcagatggta aatattatta
     4501 tataaataag ggtatggaag ggctaggatt ttatgcggca gcattgtcag gtggctcata
     4561 tcagagaata ggctttggaa aacttgaccc tatagaagta atagctgatg gaaattggat
     4621 ttcttacaaa caagctcaag atacacttac agtaataagg aattttttaa atagttttga
     4681 ctggagaaat gcatctgaga tggaaagagc taatagagct gcaaaattgg taaccgaagc
     4741 aaaatatgca gactctaaat attgtaacat agtctatggt aatctggttg ataaacgtgg
     4801 tgtatgtgga agttttgcaa gtagctttca tttattaaca aggttaatgg gaatggattc
     4861 actatctata ttaaatccat cattaaatca tgcttggaac tatatacaaa ttgatgggaa
     4921 atggtatagg tttgatgggt ctgaaatatc agcttttggt ggatcattag actttgatta
     4981 ccgtaaatta aaggatgcaa caagagagat gactacttac tatgatgcaa aggcattgtc
     5041 tatattagga tttaatcaat aaaaataggg ggaggcttct tatagagcct ttttttattg
     5101 atttgaaatt taataaggaa ttttatatga aaatttaatc aataaaagca aaaaaggctc
     5161 tttataaagc ctttctaagt gtttttttaa gtaaaatttt ttatatcaac atcagtttta
     5221 atcaaaacat ataacatttt ggacttactt ttaaaaataa aattattaaa atataatatt
     5281 aatcatgtta ataatgtttt tagtttatta tattctataa tagtggtcat gttgactatt
     5341 gtagttttat taagaatagt tggtttatat aaatatatgc aaagatagct tgttgtttac
     5401 tttacttatt agtatatagg acaagatact ctaataaatt tgcctatttt aatatctttt
     5461 gtactaaaac caactaaagt ttcctttatt atttcgtttt gaataataca aagcataatc
     5521 cgattgtttg tataaagact ggaaagtatc agagttattt tctaatttag atatacctat
     5581 agaacaagaa ttactctcta ggcataattc taaaaccttc tttgccttat tttcaacatt
     5641 ctcttttgat tgattactat attcaaaaac agcaaattca tctccaccaa gtcttccaat
     5701 aatagcatca tttacgaata ctttagtcaa aatatttgaa aaatgtatta gtatttcatc
     5761 tcctcgaaca tgaccaaaga tatcatttac ctgtttaaaa ttatctaaat caaagatgta
     5821 aaagtatcca tgtgaaacag ttttaagcat tttttttatg tgtacttcaa aaaaatgtct
     5881 attgtatagt ccagtcataa ggtctttttc tgattttagt ttaaattcat ttgccattgc
     5941 tagtgcttct tgtgcttttt cagttattgt tttagggtaa tactcatctt tcatttggtc
     6001 aaagtttgga atagaatggt gaacatggat aagtttaatc atatcatctg taactctgta
     6061 taccatagta aatcgagtat ccatttctac atagatatct ttttctaggt aatctttttc
     6121 tattacccaa agagtaccat atactataaa agcttcgtct gtgactttgg tacatgaata
     6181 ccactcatct ataatctcaa agtctatttg ctctgattct gtttggtctt tttcaagacc
     6241 tataataaat tcttctaatg tttcgtaaaa ttcatgtttt cctgttccaa ttaatgcaag
     6301 gttgtcatct aataaggaaa taataaattt aaggttttct tcttctggat ttaaaatata
     6361 aatatgccaa attttttttg ataaatctct taattgtttt tgtattgtat tattttccat
     6421 agattacccc cttaagattt acagtttata ttttaatgta ttataagttg aactcttatt
     6481 acaatattat tataatatag aactaaagaa attacatcct gtataggtaa aagtatatct
     6541 tttacaataa aaatattaca gtaaaatgac cagttttaat atacgtatta tatttttaat
     6601 ttataaacga atattataaa tgaaatttaa attcaacctt gaagaaataa gctaaatgta
     6661 ttagtatata aatccatgct tgaacataaa taacctttat gatatgatat caataataat
     6721 attatgagag gagtgccttg atgtcggagt tatataatgg taaccaacaa ttaaatagtt
     6781 ggagacacat atattctgta taatcagaaa tttcaagtgt atttttaata catttaattt
     6841 ttgtgtctta ttctgccttt atagataact gacatatgac aacaaatagt atttaaaata
     6901 tatattactt tgttaaatta tggtgtagta ggctttaatc tgctacactt ttattaattt
     6961 tgtatagtat tattttgata attattttga aatagagagc attaattttt ctagtccaca
     7021 ggagttttct gtttttgtgc tgttaaatat aagcacagaa aaaggcagat atatacctgt
     7081 ggactttttt ttatataaat tatgaaatat ggaggtagtt aaaatgagta taattgaact
     7141 taatgaactg actaaatctt atagaagatt tgagaaaaag gcaggattta tggggagtgt
     7201 aaagtcttta tttaatagga aatatacaga aaaaacagct atatcgtcat ttgatttaaa
     7261 gatagaagaa ggagaatttg ttggattaat aggtcaaaat ggagctggca aaactactct
     7321 tattaagatg ttaactggta ttattgcacc gtcttctgga gaaatatctg taatgggtta
     7381 ttaccctaac aaattagaaa ataattttaa aaaacaatat gctgttgtta tgggccaaaa
     7441 aagtcaacta ttctttgaat tgacaccagc agatacgttt cttttattta aagaattata
     7501 tgatatatct aatgaagaat ataaaaaaaa cttggattac tttatcgaat tatttgaggt
     7561 atctgactat ttaaatgttc aagttaggac gctttcttta ggagaaagaa tgaaaatgga
     7621 actaatagta gcccttctac ataatccaaa aatattattt ttggatgaac caactatagg
     7681 cttagatgct atagcccaaa aacaaattag gtatttccta aaagaagtaa atgaaacaaa
     7741 aggaactact attatactta cttctcatta catggaagat attaagagtc tctgtagtag
     7801 aagtattgtt gtagaaaata gtcgtaaaat atatgatggt tctacagaga aattatttaa
     7861 ttattttcaa aaatataaaa aaataacagt tagtttttcc aatgaaacta acattgtatt
     7921 tccaaaagat tgtaaagttg ttgaacaaac aaatcataag acatcagtaa ttgtgccaaa
     7981 agaaagagga aatgaaacga ttaaatggat ttttgataaa tatgatgtta aagatgtaat
     8041 gattgaagaa gaagatatag gtagtattgt agagagaata tataagaaag gaagtgatga
     8101 gtattatgag caactataat gaagcaagtt caaattattc aatgttttat aaatttaaac
     8161 agagtattag aaggtattat gaagtagcca aaattaattt taaagtgcaa cttatctgga
     8221 gatttgatgt ggtggttaat atggtgttta cgattgtaaa aattttattt gcttatattt
     8281 tatggaaagc aatttttggt gaaaataaaa tagtctctgg ttttacattt aactctatgt
     8341 tatcttacta tataataagc tcatttttag caggtattga tatgtcaaag agtaccagtg
     8401 aagagatatg taatcgtgta aaattgggca ccttttcaaa gtacatgatt attccaatta
     8461 atgttcaaaa ttattttatt gctaaaaatt ttggaatgac atgtttttat tttgtattta
     8521 atatgatttc tgcatttgta tggattttta tatttaaaat agattttgcc tttacatcaa
     8581 atccagttca aatattaatt tcaattttga tgatttttct agggtttatt tttatgatga
     8641 aactccactt ctttattggg attttgtcat ttaaatttct tgagattagt ttttttagaa
     8701 tgataattga aaatattgtt gaatttatta caggaacaat gataccactt gtactcttac
     8761 cagaaactat agtaacagtt atgaaggtat ttccatttta ttatacaaca tatttgccat
     8821 ctatgttact aataggtaga aacagcaatg aggcaataca aggattaatt attctagcat
     8881 tatgggtttt atttttttct gtagtaaata aagttatgta taaaagaatg agaacattat
     8941 ttgatggggt gggtatatga tgaaaaaaaa tttgaaaata atatttatgt tattaaaaat
     9001 gaaattatca aagatgatgg tatttagatt tgattttttt ggagcatttt ttgtagatag
     9061 ttcattattt atattacaac ttctaatgtt taatagtata tattctcatg tagactcaat
     9121 agggggatgg aaacaaggag agatgttaat atttatagga actttctcat taataaatgc
     9181 gataaatatg acgattttct tttttggtat ttatgatatt ccacgaaaaa tccaggaagg
     9241 agaattagac tactatatta caaaaccatt agatccacta tttaggttaa catttgaaaa
     9301 tattaatatt ggttcatcac cattaattct agcaagtata cttattgttt tatatggaat
     9361 atctgagtta aactcagaga taactttagc tatgataata atatatattt tcatggtgtt
     9421 attgatgaca ttactttttt atgatgtatg tatcatactt agaacagttt cattttttgt
     9481 tatatcatct tctgcgataa tgagattaga agataattta ttgccaatga atatgaaaat
     9541 tcctggtgta atatatgaag gtgtttttaa agtattattc tatttaatct taccatatgg
     9601 aattatgtcg acaattccga ctcaaatttt ggctgggaca ataacaatta atggattaat
     9661 atactcaatt ttaattgtat ttttgtttac attgtttatg tggagatttt ggaagttagg
     9721 tatgagacat tataaaagtg caagtagtta ataaattagt aaaaatagac aatgtataac
     9781 tatatataaa tatgtttcat atgacactaa actatgtgaa caagtatttg cgctaaaggc
     9841 actgaaagct ataaaattat taaagtaagg atttaaaagt ttctttagta gaaatataat
     9901 tgtgtaaaaa agcaagatta attaaaacta tatccaaata attagatata attcttatgc
     9961 agaaaggagg tctgtaaatg tctaattgtt tggaatatat tttaaaaata attaatgtac
    10021 agaaaaataa acaaaatgag tgatgtatat tattcagaaa atcatgaaca agataatctt
    10081 tatgagcaag ataatttact ggcacaaaga tttaatttat ttaccctatt gaagtttgca
    10141 tttcctacta tttttatgat gatttttatg gggttgtata caatcgtaga tactatattt
    10201 gtatcaagat ttattaatac aaatgctcta tcagcaatta atattgtatg tccaattatt
    10261 aatcttatag ttggaattgg aactatgctt gccactggag gaagtgctat tgtagcacgt
    10321 aagatgggaa atggtgatat tgagggagca aataaagctt tttctttaat cgtattatca
    10381 ggagttgtaa ttggtgtaat aattactggt ataggtattg tatttatgga ttatatcatt
    10441 atatggttag gagcaagtga agttttaatt aaatattgtc gttcatatct ttttattatc
    10501 ttggtttttg cttctgcaaa tatactacaa gtattatttc aatgtttatt tgtaacagct
    10561 ggaaagcctc aatttggatt atggctagtg ttaggtgctg ggtgtgctaa tataattctt
    10621 gactatgtgt tcattgttct gttacaaatg ggtattagtg gagcagcact tgcaactgga
    10681 gtaggatacc taattcctac cgtttcaggt atggttttct tttcaaaaaa caaagggtta
    10741 cttaagtttt gtatacccag actagataaa agaatattat ttgaagcttg ttttaatggt
    10801 tcatctgaaa tggtcagtca actatcaaca gcagttacta cttttttatt taatgctatg
    10861 atgatgaaat taattggtga agatggggtt gcagctatta caataattat ttattctcaa
    10921 tttcttttaa cttcgcttta tataggtttc tcaatgggtg ttgcaccaat aataagttat
    10981 aactatggaa gtgaaaataa tacgcagtta aatcatgttt ttaagatttg tatattattt
    11041 atttcagtag catctttatt ggtatttatc ttttctatat catgttcacc aatcattgta
    11101 gagatttttt ctcaaagagg aagccgagtt tatgaaattg caaggtctgg attttatata
    11161 ttttctttta gttttatttt ttgtgggtat aatattttta cttcgtctat gtttacagca
    11221 ttatcaaatg gaaaggtgtc tgcaatatta tcttttctac gtacatttgg atttataact
    11281 ataggtttat tgatattgcc aaatatatta aatataaatg ggatatggtt agcagtacca
    11341 agtgctgaga ttttaacttt atttttaacc attttttgta tatgtaagta taagaataga
    11401 tataaatata tgtagatatt aaataaatag tttaggtaga tattttatgt tttaataagt
    11461 aattcaatta gtgggagata tatactattg aaaattaaag ttataattga tataaagaat
    11521 taataaaaat aacatataaa agtttaaaag ggagttgaac ttaaagtttg attagaaaaa
    11581 gtcaatttta agacaactcc ttttatttta ctattgtatt aatctgtatc tgtaagtgta
    11641 ttagtctaat attataaact ttggatgaaa ttaattttct ataggtttta atgtatcata
    11701 cactttattt taaagaaact tatcttaatt ttcatagtga ttgaatttgt ctattagaga
    11761 tgttactatt agtaatgtaa aagcaagagt agtgaatata aaaaacaaga tagtgatact
    11821 gcaagattag aatataataa atttttatgg agagatttca atatggatac atgttttaat
    11881 aaaaaaatat tacttgttga tgatgaaaag gatatagtag atttaattga agaagtactc
    11941 ataaatgatg gttttaaaaa cattattaaa gcttataatg gtctagatgc tatttcgctg
    12001 tgtaagatag catgtccaga tgttgtcatt cttgatataa tgttaccaga tatagatggt
    12061 atagaagtgt gtaaaaaaat tcgagagttt tcatactgtt ctattctttt tttatcatca
    12121 aaaaatgatg atattgacaa aatactaggt cttagtagtg ggggagatga ctatataaca
    12181 aagccattca gtccaagaga aattgctttt cgagttaaag cgcaattacg tagacaacag
    12241 tatcaaagca ttgtaccatc agatagtgaa gttattaaaa taggagatat tactattgat
    12301 atagaaggaa accgtgtata taaagataga aatgagattg agttaactgg aagggagtat
    12361 catcttttaa gctacatggc tcaaaatgta aataaaatta taggaaaaga aagactctat
    12421 gaacaagtat ggggagtata tagtagtatt tgtgacaata caattatggt gcatatacga
    12481 catataagag aaaaaattga agataacccc tcaaatccta aaatacttat aacagttaaa
    12541 ggactaggat ataaacttgt gaatagaatc gattagaata gattttaata ttaatgaaga
    12601 tatctcaagt tgaattttta gttaatgagg cattctttta tgggtgaaat caaatgtatt
    12661 ttcgttatct caacaatgaa gaagaggtct atctctattt tgagatagcc tcattaagtt
    12721 tatatcctgg taatgtactt tatgttctaa taaaattttt ttagtttatt tgcagtgaaa
    12781 cattaactag aacattaaaa tttgcattga aataccaatt aaataatatt gtatattgtg
    12841 tataaactta tactaattgt ttttctatat taaatacttt atcaatccaa cctttataaa
    12901 atttttcttc atgggaaact aggattatac ttcctctaaa ctgtttcaaa gcattttgta
    12961 aagcatcctt tgtctctgca tcaaagtgat tggttggttc atccagaata agaaaattac
    13021 aaggagataa catcatacaa cataatttaa ccttagattg ttcaccacca cttaaagtag
    13081 atacactcct tgaaacatgc tcttccttca caccacatct ggctagatga cgtctaatct
    13141 cttttgtatt taattttgga tatttatctg ctactatttg aagtggggtt ttatcaggat
    13201 tttcccattt taaatcttgc tcataataac caattttaac ttgttctgaa aactggaaat
    13261 ctccagatat acgaggaata tctttgacta atgtctttaa caaggtagat ttaccgattc
    13321 cattaaaacc agttataact attttttgac ctccatcaac agagaaattt aattttggaa
    13381 gtaaagaata gtagtatcca acttcaagat ttgttatagt aagagcattt tgagccgata
    13441 tttcaatttc tgaaaattga atatttggtt ttcctgtaaa actaggagga gctattcttt
    13501 ctattttatc caattgtttt tgtcttcctc ttgcaatttt tgaatttact ccagctttat
    13561 ttttacgtat aaatgtttct tctttttcta ttttcttttg ttgtgcttga taccttctaa
    13621 tataatcttc tcttaaatgt gatttttgtt taaggaactc tgaatatttt ccatgatatt
    13681 tcttaatcat gccaaattct atatcaagaa tacctgtaga aattttttct aaaaagtcaa
    13741 aatcgtggga aactacaata aatgccccat taaatgtatt taaataattt gagagccatt
    13801 caacatgctc tttatctaaa aagtttgttg gttcgtctaa taatagaaca tcatggttag
    13861 aaagtagtaa ttttgctaaa attacctttg ccctttgacc accactaagc tctctaacaa
    13921 cacgattcat tcctattgaa tctaatccaa gaccatttgc tattttattt atttcattat
    13981 ctatagaata gaaattactt gctattagtt gttcctgata atctgatgct ttgattagat
    14041 attgctcatt ttcagatata gcactttttt gatagagtaa attcatttcc ttttctattt
    14101 tataaagttc ttcaaatgca gtgtgtaaat agagtgatat tgttgtatct ctattgattt
    14161 ctgcatattg gtctaaatgg cctatattga tattaggttg ccacttgatt tctcctgaat
    14221 ctggaacaac ttctcccaat aaaattttaa ttaatgtact tttacctgta ccattttggc
    14281 caactacacc tatatgttca cctttgaata aatcaaagtt agctttctca tacagtgatt
    14341 tatccataaa acattgtgat aaattagtta cttttaataa actcataatt caaactcctt
    14401 ttcttttatt aaaaaattta tggaaataaa aaaagcagag gaatggcatt gtaaaatgcc
    14461 gacctctgct tttggaaaaa caaaaggtca tagacaagca ttgttgtcta tgaccagaaa
    14521 atctatatta tctatcggca aataggtaat tttaaataag catagccaaa aaactatact
    14581 aaacctatat ttgccgatga agatagaaag ctttaagctt tcagattaac ctctacacaa
    14641 cgcttcatcg tatacatttg tgtagagtta agttttctaa caagtttaac taattctata
    14701 taaatcataa ttattcacat cctaatctta gtattaattc tagtttatct gtatttaaat
    14761 tgtacttttg tactttgtgg attatcttat ttatttaaaa tatgttttat taattatagc
    14821 ttaagttatg ttatcataat acttagttat agtcaataag aaagttatat ttactattat
    14881 tatattaatt tagtaaatct atcattacaa agttatttga agactcgcta tcaataaaat
    14941 tttatagtct ataagaaaat taagattatg agttttataa ttttgatata gggatactat
    15001 tttcataaat attacaacat gtagaagtat atgtctatta ttgttgaatt ttcaatgata
    15061 tattaaggtt ctaatatatt atacaaaata aaaacattaa ttcaacttaa ataattttaa
    15121 gtattatatg tgttttgatt aaagaaaact aaaattttaa ggaaaaataa catatttgta
    15181 ttaaatttta ttgaaatgat taaatttatt tacttaatgt aaattattag ctaaaattgt
    15241 attaaataat taaggaggtg agtagctctg gatgaaatag atttaaaaat aattaattct
    15301 ctaaaaaaga atagtagggc ttcaacctct gaaataagca gacaaattaa tttatcagta
    15361 ccagcagtct cagagcgtat aaggaaaatt gagcagtcaa atttaattca aaagtacact
    15421 attcaaatta atcgggaaaa aacaaaatat aagcttttgg cttttatatt tatcaatact
    15481 gatttatcta aaagtatgaa agaatttaag aaaactatat tggaatatga tactgtatta
    15541 gaatgtcatc atatttcagg agagtatgat tacttaataa aagtattagc agaagataca
    15601 agtaatcttg aagagtttat atcagtgact ttgaaggaaa ttggggggat tcaaaaagct
    15661 aatacaacca ttgttttatc tacaattaaa gaagaattta atacaataaa agatattttt
    15721 taaataattt actagtaaag tgttaattat attttaggag gaaaatagaa tatgaaagtt
    15781 ttagtttttt tagcaaaagg ttttgaaact atggaattta gtgtatttgt agatgttatg
    15841 ggatgggctc gtaacgatta tgggcatgat attgatgtag tgacttgtgg atttaaaaaa
    15901 caagttatga gcacatttaa tatacaagta ttagttgata agacgataga agaagtatgt
    15961 gttgatgatt atgatgctct agctattcct ggaggatttg aagaatttgg attttatgat
    16021 gaagcatatg attcatcttt tttaaatcta ataagggagt ttaattcaaa agaaaaaata
    16081 atcgcttcta tttgtgtggc agcattgcca gttggaaaaa gtggtgtttt aaaaaatcgt
    16141 aaagctacta catatcattt gaaaaatggt aaaagacaaa gacagttaag tgagtttgat
    16201 gtaaatgtag ttaatgaacc aattgttgta gataaaaata ttataacctc atattgtcca
    16261 gaaacagctc cacatgtagc ttttaagctt ttagaaatgt tgacatctaa agaacaaatg
    16321 gatgaagtta aattagcaat gggatttaaa ctgtaatggt ttatagattt tctaagtaat
    16381 cagaacccca gtcacacatg gaatctaaaa ttggaataat actctttcca aaatcagata
    16441 gcgagtattc tacttttgga ggaacaacag gataaaccac tcttataatt aaaccatctg
    16501 attctaattc acgtaattgt tgagttaaca tcttaggcgt tgctttagga attaattttt
    16561 ttaattcatt gaacctcaat atagtgtctt taagatgcca aagtataagt gtcttatatt
    16621 taccaccaat caatgcaagt gttgcttcta ttgggcaact gtaattatct aaacattttt
    16681 tcatttttgg gctcctttat ttattttaaa gtatcttttt aggtactata ttacaaaaaa
    16741 gtgcattatt gtaatatagt accatagtga taaaattata gcataggtta aataaatcat
    16801 caacttagcc taataaaaca atagggggta aataaatgaa tttcgttgaa cttgcaaaaa
    16861 aaagatattc ttgccgtaat tatcaagata gaaaggttga aaaagaaaaa cttgagaagg
    16921 ttttagatgt tgcaagaata gctccaactg gtggtaatcg tcaacctcaa agattgattg
    16981 ttattcagga aaaagaaggt ataaataaac tttctaaggc tgccaatata tatgatgcgc
    17041 cattagcaat tcttgtatgt ggagataaag acaaagtttg gacaagacct tttgatggga
    17101 aacaacttac agatattgac acaagtatag ttacagacca tatgatgtta caggcaactg
    17161 aacttggatt ggcaagtgtt tgggtgtgct attttaatcc agatataatt agagaagagt
    17221 ttagtttgcc agataatcta gaaccaataa atatattatt gatgggatat gaatctaaga
    17281 taccagagag cccagagaga catgaaaaaa ctcgtgttcc tttaagtgaa attgtttctt
    17341 atgaaactct ttaattataa aattaagtta ttcataggat aaatgtaata aagtgtttgt
    17401 tataaacaaa attcaaaata aataatatct acatataatt aattttagtg aagtatatct
    17461 agtttaacta gaatgtatac ttcactaatt tatttattca aaatatttat gaggatttta
    17521 atatattaac taaaatatat caattgaatt aacttttaaa tctggaaaag cacttgaaat
    17581 atagtgtttt atatcattta aatggtcttg aatttgcttt tctgatattt tatcaggtcc
    17641 atatacaaaa tacataatat ttttagtatc ttttgttata gctgttcgat tatcagggcc
    17701 attaagaata ctagataata taccaatatt atctgataaa aataaatcat cttttgttaa
    17761 aatatgttct ttaccaccta taccataaaa atgttcactt ccatttgcta attctatttt
    17821 caaaggtaat tccattttat caaggtcatg acctgctgtt agaagaagat ttttcaattc
    17881 tgctaaaaac attgactcaa ttgttacacc tacatctggg ataccttttg attttaatag
    17941 aatagattct aattgaagca atacagggta agttttcttg aattttttat aatactttat
    18001 ataacttgaa acaggctctg ttttaatttt ttctttgcga ttgtattctg ggtgttgttc
    18061 tataatattt ttaattgtag agtttttaag aactaaaaaa ttttctttat taggttttga
    18121 atgtgtaata tttataacca taactccaaa tttaatattg ggataaattt ctttaacttt
    18181 atgtgatacc tctaacataa tatagactcc tttttatttt atattttatg atataatctt
    18241 agcaaattaa aagtggattt tatagttcca tttttatagt aattttatag aaccactttt
    18301 ggaggtatta gaatgtggat tacgattaat agaaatagta atatatcatt atcaagacaa
    18361 atttattgtg agataaaaga tatgattctg gatggaagct taaagtttga gcaaaaactg
    18421 ccatcatcta ggactttatc aaaagaacta tctgtatctc gtaatacagt tttagatgca
    18481 tataatcaat taattgcaga aggatatttg gaaacaagga atggatatgg aacaattgta
    18541 gctaaaggaa tatcagggca taaagttgat tttaagttaa atgatacttt tagagatgaa
    18601 aaagatttac atactaataa acaaaaaaat tatatagatt ttcgttcagg agttccagaa
    18661 ttaaaactat ttccacgcaa agaactttca aagctatata aacaaatact gtatgattta
    18721 tcagattcgg atttcagata tggaagtaca gctggtgtat gggttttaag agtggaaata
    18781 tctaagtatt tatttcgtac tagaggaatt agatgtaatc ccaaaaacat tatgattgtt
    18841 tctggttcaa cacaaggttt atctttaatt tcacagcttt tatacaaaag taataaaaag
    18901 gttgctgtag aagaccctat tcataaaggc ttattaaagg ttatttcatc tattggatat
    18961 tcagttgttg gaattaaagt agatgataag ggaatgaata ccaacttaat aaaatcaata
    19021 aaggggattt cttttgttta tactacacct tctcatcaat acccattagg agggatttta
    19081 cctattcaaa gaagactaga gcttattaaa tgtgcaacag agaatgatta ctatataatt
    19141 gaagatgatt atgatagtga atttagatat gagggtcaac caattagttc tctttatgaa
    19201 cttagcccaa ataaagtaat ttacataggt tcttttagta aaatattatc tccagctatt
    19261 cgtgttggat ttatgttatt accagacaat attttagaaa gctataaaac cttaaaaatg
    19321 tactctgatg ttcatacaga aacaatattg caacatgtat tagctgaatt tattggaaat
    19381 ggaggtttag agaagtacat atttagaatg aagaagttgt acaatagaaa acgtaagtac
    19441 ctcataaaag ttttaaacac ggaatttaaa aatgaattta aaataaaagg tgaagcagca
    19501 gggcttcata tagttgctca tttctataat gttaaattta cggaaagttt aattcataaa
    19561 atttttgaag caggtataaa agtttatcct atacaacaat atgcaattga gaatatatta
    19621 gaatatgact atgaaattat actaggttat gctcatctta gttttgatga aattgaaaaa
    19681 ggtgtaaaaa tactcagcaa aataatacat atgaatcaag gtacatagat aaaacatgta
    19741 gcaatctaca tataatagat gtaattaagt ttaaacttta aaagaaatat atattaatat
    19801 ttttgtataa aaatacctga tattttttta tcgatacttt attgctatac actaaattaa
    19861 gatagcaatt tatattaaga aaaaactttt tgcataggtt ataatactta aaaggagagg
    19921 atagagttga aaacaaatac agatgttatt atggagataa ttgattatat agaagaaaat
    19981 cttacagaaa atctaaattt agataaaata gcttatattg ctaattattc aaaataccat
    20041 ttacacagaa tgtttacaaa tgttgtagga tgtactatcc atcaatacat acagaggagg
    20101 agacttactg aagcagctag acaacttata tttacagaca aatatattat agacatagct
    20161 attacagtag gatattctac acatcaatct tttacactag catttagaaa tttatataaa
    20221 gaatctccac aagtatatag aaataaagat gaattttatc ctattcaaca gagatttgtt
    20281 ttaaatctaa atcatcaaat tcaaagtgaa aaatgtattt cttatgcaaa tatgaattat
    20341 aaacaaagaa gagatgaaat caattcttat cttaacttta atattaattg tgaggtgaaa
    20401 gtagcatgag tttagtgcca catgtaattg aacaaacagg tcaaggggaa aggtcttatg
    20461 atatatattc aaaattatta aaagatagaa ttatatttat aggagaagaa attaatgatg
    20521 ctattgcaag tttagtagta gctcaattac tgtttttaga atcagaagac ccagattcag
    20581 atataattat atatataaat agccctggtg gctctgctac atcaggattt gctatatatg
    20641 atactatgaa ttatgtcaga tgtgatgtat cgactatttg tattggtatg gctgcaagca
    20701 tgagtgcttt tttacttgca ggaggaacac atggaaaaag atgtgcttta cctaattcag
    20761 agattatgat tcatcagcca atgggaggag ctaaaggtca agcaacagat gtaaaaatag
    20821 ctgtagataa tatattgaaa ataaaagaac gattagataa aattctttct gaaaatacag
    20881 gtaaatcaat agaagatatt aggagagata cagatagaga taattttatg actgcactag
    20941 aggctaaaga gtacgggctg attgattata taatgaatag gaatgaataa agtatcaaag
    21001 tatcatatta aaatgtctgt tttacaaata tatatataat attaaataag agtgtctcaa
    21061 aatgaacttt ttagtttatg ggacactctt ttttgtatga aattaaatat ggttttatca
    21121 tttcaacaat tattaccaaa tttcaagttt aataaattct actataaaaa atacataaaa
    21181 gataaggttc tttagtaact ttctgtcgtc aaaagttttt ataaatttaa taacaaaaca
    21241 agagaaaata ccatatatta cttatgtctg caatatatag aagacaaagg agttgtacat
    21301 tatgattaca ataagtttgt gcatgattgt taagaatgag gaagaagtta taggcagatg
    21361 cttagaatgt gtaaaggata tagtagacga aattataatt gtagatacag gttctacaga
    21421 ttccacaaaa gaaatagtct caaaatatac agacatggtg tacgatttcg aatggataga
    21481 tgatttctct gctgcaagaa atttttcatt ttctaaagct tctaaagact atactatgtg
    21541 gcttgatgca gatgacataa ttttagaagc tgatagagaa aaattattaa agattaaaga
    21601 aagcttggat acttccattg atattgttat ggcaaaatac aatgtatcct ttgatgaaaa
    21661 tggtaaccca actctctcgt attatagaga acgattgttt aaaagaagtt ctaatcataa
    21721 atgggttgac cctattcatg aagtagtacc tctttttgga gaggtatttt attctgacat
    21781 tgcaatatct cataaaaagc ttcatagaca ggaccctcta agaaatctta gaatttttga
    21841 aaaaatgatt tcagaaggaa aaacattgga gccaagacac caattctatt attctagaga
    21901 gttgtactat aatgctaggt ataaagaagc aatagaaggt tttactaagt ttttagactc
    21961 atcaagagga tggattgagg attgtatcag tgcttgcaga gatttagcca catgttatta
    22021 cttaattaat gatgaaaaaa gtgccttata ttcattgttt agaagtttcg aatttgatga
    22081 gccaagagca gaaatttgtt gtgacatagg aaagcatatg tttgatagac aaaaatataa
    22141 agaagccatt ttttggtata aggtagcatt aacaagagat aagaatgata ctaatggagg
    22201 ttttaagtca aatgattgtt atggatatat accatctata caattaagtg tctgttatga
    22261 caggttagga gaatcagata aagctattta ttatcatgaa aaaacaaaag aaatcaaacc
    22321 taacgatagt gctgttttgc acaatgaaaa ttattttagt aaatttaaaa atagttgata
    22381 ggtcacaatc atactaaatt tgacatatta tatatagagc ttaaaagctc aaatatatca
    22441 ggaggtgctt ttaataatga gtagaaataa atattttgga ccatttgatg ataatgatta
    22501 caacaatggc tatgataaat atgatgattg taacaatggt cgtgatgatt ataatagctg
    22561 tgattgccat cattgctgtc caccatcatg tgtaggtcca acaggcccaa tgggtccaag
    22621 aggtagaacc ggcccaacag gtccaacggg tccaacaggt ccaggagtag gggcaacagg
    22681 cccaacagga ccaaccggtc cgactggtcc aacaggaaat acagggaata caggagcaac
    22741 aggattaaga ggtccaacag gagcaacagg ggcaacaggc ccaacaggag ctatagggtt
    22801 tggagtaaca ggtccaacag gcccaacagg agcgacagga gcaacaggag cagatggagt
    22861 aacaggtcca acaggtccaa cgggagcaac aggagcagat ggaataacag gtccaacagg
    22921 agcaacaggg gcaacaggat ttggagtaac aggtccaaca ggcccaacag gagcaacagg
    22981 agtaggagta acaggagcaa caggattaat aggtccaaca ggagcaacag gaacacctgg
    23041 agcgacaggc ccaacaggag cagtaggagc aacaggaata ggaataacag gtccaacagg
    23101 tccaacagga gcaacaggag cagacggagc aacaggagta acaggtccaa caggggcaac
    23161 aggagcagat ggagtaacag gtccaacagg ggcaacagga gcaacaggat ttggagtgac
    23221 aggcccaaca ggtccaacag gagcaacagg aataggaata acaggagcaa cagggttaat
    23281 aggtccaacc ggagcaacgg gagcaacagg agcaacaggc ccaacaggag taacaggggc
    23341 aacaggagca gcaggactaa taggaccaac cggggcaaca ggagcaaccg gagcagatgg
    23401 agcaaccgga gcgacagggg caacaggagc aacaggtcca acaggagcag atggattagt
    23461 aggtccaaca ggagcaacag gagcaacagg agcagatgga ttagtaggcc caacaggtcc
    23521 aacaggggca acaggagtag gaataacagg agcaacagga gcaacaggag cgacaggtcc
    23581 aacaggggca gatggattag taggtccaac aggagcgacg ggagcaacag gagcagatgg
    23641 agtagcaggt ccaacaggag caacaggagc aacaggaaat acaggagcag atggagcaac
    23701 aggtccaaca ggggcaacag gtccaacagg agcagacgga ttagtaggtc caacaggagc
    23761 aacaggagca acaggattag caggagcaac aggagcaaca ggcccaatag gagcaacagg
    23821 tccaacagga gcagatggag caacaggggc aacaggagca acaggtccaa caggtccaac
    23881 aggggcagat ggattagtag gtccaacagg agcaaccgga gcaaccggag cgaccggagc
    23941 aacaggtcca acaggagcaa caggagcaac aggagcaaca ggtccaacag gtgctagtgc
    24001 aataatacct tttgcatcag gtataccact atcacttaca actatagctg gaggattagt
    24061 aggtacacca ggttttgttg gctttggtag ttcagctcca ggattaagta tagttggtgg
    24121 agtaatagac cttacaaacg cagcaggaac attgactaac tttgcatttt caatgccaag
    24181 agctggaaca ataacatcta tttcagcata cttcagtaca acagcagcac tttcacttgt
    24241 tggctcaaca gttacaatta cagcaacact ttaccaatct actgcaccaa ataactcatt
    24301 tacagctgta ccaggagcga cagttacact agctccacca cttacaggaa tattatcagt
    24361 tggttcaatt tctagtggaa ttgtaacagg attaaatata gcagcaacag cagaaactcg
    24421 attcttacta gtatttactg caacagcttc aggtctttca ttagttaata ctgtagcagg
    24481 atatgcaagt gcaggaattg caataaatta gattagataa gaaaaggctt ctaatatcgt
    24541 attgatatta ggagcttttt taagaatgat tgatgaacat agttttaata ttaattagtg
    24601 cgcatattat aaaatactat tatataatat aaaataataa cattaaattg gaaatataga
    24661 attatctagg ggtgatacta tgaaaaataa gattgaaata tcaatgaaca actttgtaaa
    24721 aaattatcat aaatcaaata atataactac aaagtggcaa gaacttttgt ttgcttatgc
    24781 atctgctgaa gatgatatgt ttcagaagct aaaaagtgtt gtaggaacat ctcatgctat
    24841 gccaaaagat tttctggaaa atggaaaaac tataattaca tattttatac cttttgatga
    24901 aagtgtaaat aatagtaata ttggagggtt tgaatgttct gaaatatggg caagagcgta
    24961 catagaaact aatatgttga tttcaaattt aaatgatttc ttatgtgaag aactagagaa
    25021 gatgggggct aattcatatg ctattcctgg gtcgtataat tttgacagtg aaaaattggt
    25081 aagtgactgg tcacaaagac atgtagcatt catagcaggt cttggaactt ttggtttaaa
    25141 taatatgctt attacagaaa aagggtgttg tggaagaata ggaagcataa tcacaagttt
    25201 agaattagag ccaactaaaa gaccagaaaa tgaatactgc ttgtataaac ataataatag
    25261 ttgcaaaaaa tgtgtcaaca aatgcgtgaa agaagcttta aaagaagatg gatttaatag
    25321 gaataagtgt tatgaaatgt gtatgataaa tgaaaaaaaa tacacttata taggtggatg
    25381 tgatatttgt ggaaagtgta tagttggagt tccttgttca gtaataaacc catcaagata
    25441 atggatttaa gttgactaat aattttaaag atagtttata atgaaaaaat tttagttatc
    25501 aaaataaata taatataaaa actaaataaa tgtgtttaaa aaatggataa ataatttgtg
    25561 ttgattgaca accagttata ctgttgatag acttatatgt atcgctgtga tatataagga
    25621 gggttgagat tttatgtttg atttaatctt tatttttgca tagcaagggc tattgcaact
    25681 atgaaattta atatataaat agccctttgc tattcctaat gaaaaattac tatttaatag
    25741 gagagcaaaa tgagctataa aaaagcaata catatattac cagaagaatt acttgaatta
    25801 atacaggaat atgtagatgg tgaatgtatc tatattccta gaaaatctaa taataaaaaa
    25861 gaatggggaa gtaatacacc tactcgtgag gagttgtcaa ttagaaatat gcagatttac
    25921 gaagactatc aagttggata tgatttgcaa tatttatcgg aaaagtatta tttatctttg
    25981 aaaagtatcc aaagaattgt attacaagaa aaaagaaaag atatctaagg atgagccagt
    26041 atagttttat aattatactg gctcattttc ttttgataaa agtgtttgag gtatagtttc
    26101 ttttaagtgg atgatataat ttaagtaatt tcaataactt atacagtagc taacaaagct
    26161 ggttgtaaag tatctaataa gaagtagtag ctttttatat gtttgaaatt aaaatttatt
    26221 aaaattagga ggtcttgttt tgaaaaaaat acttgattat attccacctt ttcataactt
    26281 tctattaatt tagtaatctt atagaaagga aattgcattt atgaaaaaaa ttacttggga
    26341 ggtggaatat atctcccagt tacctgtaat taggtattgt aaaaaatgtg gaaaaaagac
    26401 tgaatatatt tgttcaggtt tatttagggt taatgctcaa cataaatatt tggatatatg
    26461 gttaatatac aagtgttcaa attgtgattc aacatggaac tcaactatat attctcgtgt
    26521 taatccaaat agattaagta cagaaacact agaacaattc catacaaatg atgataatct
    26581 tgctaatcaa tatgctatga atgtagaact tttacataga aatggagcag aggttggtat
    26641 tccagaatat aaaatagtag gacaagaaat tgatattaat gaccttacac aagttcgtat
    26701 tgtaagtaag tatccatccc aattaaaggt gtcagcatta ttacgtgaaa aactaggttt
    26761 atctaggaaa gcatttgatg aaatgttaga ttgtgggact attaaaagta tatctggagt
    26821 tgatttaagg agaagtaaat taaattatga aattgtgtta gaggtaggca agcagaagca
    26881 aaagtcttca aactgattag aataactaac ttaatatatt ttcaggaaca tcatttaaaa
    26941 aaatagtgac tttaaaaaat ttaaagccac tattttttaa tatgttttat atgagtattt
    27001 aaaataaagt gtgtattctt caaatttata gcgataatta aaacattact ataaaaagga
    27061 aggtgcatac tttttattta acaaaaacta gatattaaaa acttttcgag gaatatttta
    27121 ttaaaatccc taaatatatt taatatatat taaaaaattg tattataatg aaaatcaaaa
    27181 tagattgcaa atccaaaaag taatacttaa tgtccaaaaa gctaaaatta aaatctaaat
    27241 agctatttac tacagaagta tgttaaaata tgtaaaatga atagaaattt atatattttt
    27301 attaaaaata tataagaaag gatgtttaag aattgtgatt aatataggaa tatgtgatga
    27361 tgaactacat tatcgaataa aaataaaaga tatattaagt gaaatattaa gttcatatcc
    27421 tataaattat aacatatatg aattttcagc aggagaagag ttactaaata actatcctaa
    27481 agatttagac atattaatta tggacataca aatgaaaaca ataaatggta tggatactgc
    27541 taggaagata agagagtttg atcataagtt agaaataata tttgttactt catttgtaga
    27601 gtttatgcaa gaaggttatg aggtaaaagc atacagatat atactaaagc caataaataa
    27661 agaaaaaatt tctaaaagtg tattaccttg tataaatgag atgatgaaaa agagaaataa
    27721 ttacttaact ataaatgtaa aaaattatgt agaccgaatt aaaatagatt ctattactta
    27781 tatagaaaca gatagaccga atatattgat ttatacacat gatgatatgt atattactaa
    27841 aatgagtata tctaaaatag aaaagatatt aaatgaatat ggatttttta gatgtcataa
    27901 tagctatata gttaacttaa aattagtaga atcaatgagt ggaagtactg ttatagtaga
    27961 tggaaaaagt attccaataa gtaaatatag ggttaaaggc ttgaagcttg cgataactaa
    28021 tatattaggg gacatagtat gctaacaaat ttaagtgtag gattttggaa tattattaat
    28081 ttagtttcag gtactataga ttggattata ttttttttag taatccactt agttggaaag
    28141 aggaaaatat ctaatataag atatatattt acaa
//
LOCUS       DAGSRM010000041        27980 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.041, whole genome shotgun sequence.
ACCESSION   DAGSRM010000041 DAGSRM010000000
VERSION     DAGSRM010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27980
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.041"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(81..365)
                     /locus_tag="MJE33_002000"
     CDS             complement(81..365)
                     /locus_tag="MJE33_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647934.1"
                     /translation="MLVYNKSFYPNDIFPRLDFSKIKKQLKLIDNDLSDFGSICIIEK
                     EHYTISVNSIGEINVYYDLEYENKVYRIVYEIEKLFKSQVGRFSISTYRN"
     repeat_region   534..2007
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=600..628
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(2838..3710)
                     /locus_tag="MJE33_002001"
     CDS             complement(2838..3710)
                     /locus_tag="MJE33_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBY9647935.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
     gene            complement(4104..4682)
                     /locus_tag="MJE33_002002"
     CDS             complement(4104..4682)
                     /locus_tag="MJE33_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBY9647936.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYTDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            complement(4740..5456)
                     /locus_tag="MJE33_002003"
     CDS             complement(4740..5456)
                     /locus_tag="MJE33_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBY9647937.1"
                     /translation="MRINKYIASCGIASRRKAEEIILGGRIKVNGSVVKELSFNIDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVRERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKTYVASVKGIISDDEIRQFETGLKIEDYTT
                     APAKIRVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
     gene            5647..6732
                     /locus_tag="MJE33_002004"
     CDS             5647..6732
                     /locus_tag="MJE33_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBY9647938.1"
                     /translation="MIIISKENIKYYLIVVFIGILFFKFINTPSDFISSIEGFLKFFS
                     PFLLAILLALLLNPLVMLFEVKFKAHRLLAIFLSYIFIGFILAFAIRLLIPSIANTLN
                     RLINEMPMYTDYIDSFIEKNMSNIDFLKTLIPHIQHSLDNLLKEASNFVGKIPKNFLI
                     YTLSISSMLFNMTMGFILSIYIIYDKEKIALGFKRFLYSSTARNKADNIIEFFRTTHD
                     IFYDYLLGRILDSLIIGIIAFLGFQFVIRIENALFLASIIFLGNIIPYFGPFIGAIPP
                     IAMTILYSPQKTIWVIAFLFILQQLDGNFIEPKVMGNQVGIGAIWVISAILVGQSLFG
                     FIGVFLSVPIAAVVKTYVDKYIDNRLQ"
     gene            complement(6887..7546)
                     /locus_tag="MJE33_002005"
     CDS             complement(6887..7546)
                     /locus_tag="MJE33_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBY9647939.1"
                     /translation="MRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLEKHNYKK
                     AMESLSSALEEDSTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEKIKNGSS
                     VIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYSGSSKNN
                     KSSSKNNDKKSDSNKSDNNNSSSQNQEKPNNSNPTPAPTPQDTTGGSDSGGNSQNSNN
                     Q"
     gene            complement(7763..9055)
                     /gene="lysA"
                     /locus_tag="MJE33_002006"
     CDS             complement(7763..9055)
                     /gene="lysA"
                     /locus_tag="MJE33_002006"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBY9647940.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYVEYFKAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSEYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIQLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHEAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEI"
     gene            complement(9073..10266)
                     /locus_tag="MJE33_002007"
     CDS             complement(9073..10266)
                     /locus_tag="MJE33_002007"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBY9647941.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHVVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     gene            complement(10627..11244)
                     /locus_tag="MJE33_002008"
     CDS             complement(10627..11244)
                     /locus_tag="MJE33_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647942.1"
                     /translation="MTNMEKEILLLISRVRCVTESQFRKIYGDQKRYNKKNFKKTLRK
                     MCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSELVVRINTAGCD
                     VKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYENLSKKIDKSTI
                     PFFKVPNVLVVTPDNLSELNDLERYDNEYSINFIDLGLNKLFNCI"
     gene            complement(11414..13114)
                     /locus_tag="MJE33_002009"
     CDS             complement(11414..13114)
                     /locus_tag="MJE33_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647943.1"
                     /translation="MKRYLKTYGVALIIVIIYAFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVLHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQSFVTDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNDLKDEVLEGTVTRISPDVRGGNTNYYVTLDSNKDIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEKTGTDENTDSKDSENQGNV
                     "
     gene            complement(13511..14074)
                     /locus_tag="MJE33_002010"
     CDS             complement(13511..14074)
                     /locus_tag="MJE33_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647944.1"
                     /translation="MNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYSF
                     GRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVDNLQYENLYKNIKLISDYRE
                     DYYYDTMALIYLLMNIQRQSEYKYVCSNFVADMLEKSEIDILNKQAFEVTPNDFYNLS
                     GLTLEYEGLLSEYNSRQHSYVSKIANI"
     gene            complement(14233..14946)
                     /locus_tag="MJE33_002011"
     CDS             complement(14233..14946)
                     /locus_tag="MJE33_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HBY9647945.1"
                     /translation="MGKVAKILLSLIVIVLVLVGIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKVFGLVNSQYELELRPTLKDGNLVVSLENVKIGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPITLKNISIKKGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKVQDILNHLINLKKTSVG"
     gene            15238..16902
                     /locus_tag="MJE33_002012"
     CDS             15238..16902
                     /locus_tag="MJE33_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HBY9647946.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLEQINPNSLEILQGFVEPTQ
                     IKDAKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPGK"
     gene            complement(17009..17908)
                     /locus_tag="MJE33_002013"
     CDS             complement(17009..17908)
                     /locus_tag="MJE33_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBY9647947.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEKNYLLIPYRLTIKE
                     PVKNAFKEVDSSYEHILIAGGDGTVDLVVNAMKELDIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVHIECEDSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(17935..18573)
                     /locus_tag="MJE33_002014"
     CDS             complement(17935..18573)
                     /locus_tag="MJE33_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBY9647948.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KAGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKDIKPEEMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKTTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
     gene            complement(18913..19911)
                     /locus_tag="MJE33_002015"
     CDS             complement(18913..19911)
                     /locus_tag="MJE33_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 domain-containing protein"
                     /protein_id="HBY9647949.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KRISPRLVEERIELIKKNTGLFSNFRGYNMFYMATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYDYMRKKHPFLTSSDDYCNIAL
                     IAIHSKNLDEDLEYIEKCYEFLNENGFYKGNDLQALSQILLFSDDRTMLKCKKTIELK
                     KAFKENDCKMNYYGYPIIGAISLLDYREDEIIEEIKNVSNKLKEEKGFGNWSLGKSNR
                     VMISSAIVASIYADLIRNENNIGSITNNIFLNIVVAIQIACVMAATSAAIASSSSN"
     gene            complement(20009..20584)
                     /locus_tag="MJE33_002016"
     CDS             complement(20009..20584)
                     /locus_tag="MJE33_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647950.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKIKKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
     gene            20900..21163
                     /locus_tag="MJE33_002017"
     CDS             20900..21163
                     /locus_tag="MJE33_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647951.1"
                     /translation="MIKNTKQITVEVNEILLQEMSKITNDENNILTDLINESIVNYIL
                     ENKKHNIENKDLEEVIKFYKKENTEKPKSAARAIAEAKTHTLY"
     gene            complement(21600..21977)
                     /locus_tag="MJE33_002018"
     CDS             complement(21600..21977)
                     /locus_tag="MJE33_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBY9647952.1"
                     /translation="MTITKIPPAELKVMKFIWSSDFTVASKDVIEAMEKLYNWKQTTT
                     LTLLSRLVNKQFLSARKIDRHTHYTVLIGEEEYLHFETKTFLDNMHGSSIESFMKSLF
                     KKGVNEEELNSVEKWVKDIKKDE"
     gene            22318..23430
                     /locus_tag="MJE33_002019"
     CDS             22318..23430
                     /locus_tag="MJE33_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBY9647953.1"
                     /translation="MNAFIRKRNKNYVVYLEFEDEKTGKRKQKNMGTFDKKREASKRL
                     NEVKESLYNDGFLVPSEITVSEFLLDFLNKYSENISLATYNNYVSICKNYINPSIGKY
                     KIQDLHPVHIQNYIDKLSYKLNPQSIKIHINILKLAIKRAYRLKLIKENVMDSIEAPR
                     YKKFKNEIYDREQMIKLLDLAKNTEMELPINLAIGLGLRISEVLGLTWDNIDFEENTI
                     TVNKITSRINGSVILKEPKTESSVRKISSPKELMSLLKEYKIKQNKNLLKSSIRNNNN
                     LLFFNKKCEPIAEDVMSKKFKRFLEKNELPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
     gene            complement(23638..24114)
                     /locus_tag="MJE33_002020"
     CDS             complement(23638..24114)
                     /locus_tag="MJE33_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647954.1"
                     /translation="MVKKRLVAVVISLAMISVGNFSYAHSGGHNSSGGHHNKNKSELT
                     RYYCGGHSAHQHNKGFCPYASTKENSGVTISSVHNEKNHKNYFAEKGYNKGYEDGCKG
                     LYNLSVTEAKSDNLYEKEILKSSYEKGYLIGYNEYKAKKVDDVMKKMLDIPMLASI"
     gene            complement(24313..25938)
                     /locus_tag="MJE33_002021"
     CDS             complement(24313..25938)
                     /locus_tag="MJE33_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBY9647955.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALFNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLESKEIQDQLAKMYGKDK"
     gene            complement(26276..27295)
                     /locus_tag="MJE33_002022"
     CDS             complement(26276..27295)
                     /locus_tag="MJE33_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HBY9647956.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLVIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKEII
                     KSAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
     gene            complement(27292..27861)
                     /gene="mocA"
                     /locus_tag="MJE33_002023"
     CDS             complement(27292..27861)
                     /gene="mocA"
                     /locus_tag="MJE33_002023"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HBY9647957.1"
                     /translation="MINAVIMASGFARRMGANKLLLNYKGCSIIEHVFREVSKIDFHQ
                     VVVVSQYEEVLLLCNKYGFEYIDNKNANIGQSESIKLGVLNSSKCDGYMFFVGDQPFI
                     DNLYIDKIINTFKLDRDFIVIPRYKDMCGNPVIFPYSKKEELLALKEDEKGKTILKNS
                     SKIKYVEVPKEMLFDIDTKMDYERIGGKL"
BASE COUNT         9422 a         4583 c         2867 g        11108 t
ORIGIN      
        1 aacaactctc tctagctgtt acctcatata tttatctata tctttagtat taagtttttt
       61 aatttctact tttttaatta tcaatttctg tatgtagata tactaaacct tccaacttga
      121 gatttaaata acttttcaat ctcataaact attctataca ccttattttc gtactctaaa
      181 tcataataca catttatttc acctatactg tttacactta ttgtataatg ttctttttct
      241 attatacata tgcttccaaa atctgacagg tcattatcta tcaattttaa ctgttttttt
      301 atttttgaaa aatctaatct tggaaatatg tcattaggat aaaaactttt attatataca
      361 agcatttcat ccacccctta tttattctat ttatatcatt ttatacccta attaataatt
      421 tattaatcat ctttttccat attattcaaa ttctcttata taaaaacaaa atcaatttag
      481 aatatgtatt attaaatata aagtgactca atctttattt aataagaaaa aacatttata
      541 ttacatatag ttaatataaa actaatagtt aatgaagcat tgcaataggg gggtattgta
      601 tttacattcc atatagttaa tataaaacgc caagtacaat aataaaaaat aaagaaactg
      661 taatatttac attccatata gttaatataa aactagtaga aagaaataaa ctttttttag
      721 ggaataattt atttacattc catatagtta atataaaacg atttgtaaca ttaaaaaaga
      781 ataaaacttt ctcagattta cattccatat agttaatata aaacgaataa ccaaccccta
      841 attttttagc aatttgttcc tatttacatt ccatatagtt aatataaaac gatgtaataa
      901 atgagtattc agtgcatttc aaaaattatt tacattccat atagttaata taaaacattg
      961 tatgatttat ttttatagcg gtactcctgg gagatttaca ttccatatag ttaatataaa
     1021 acttattcca agaaggacga gttttagcat tccaagaacg atttacattc catatagtta
     1081 atataaaacc attatgctat agtttcctag ctctccgaca cctacattta cattccatat
     1141 agttaatata aaactacacc agattataat gaaaatggag cattagggaa atttacattc
     1201 catatagtta atataaaacg agtctaaatt acaagcagaa gcggaaaaaa tgataaattt
     1261 acattccata tagttaatat aaaacgtggt tggaacttat aaaaaagatg ctgataagtt
     1321 atttacattc catatagtta atataaaacg ttctgttgca acttttgaaa tgtattgacc
     1381 aagtatattt acattccata tagttaatat aaaacaattg ataagcaaag tcaaaaataa
     1441 acattccttt tggtatttac attccatata gttaatataa aacggatttt aaacctcctt
     1501 ttgatgttcc tgctagtaga tttacattcc atatagttaa tataaaacgg agattctggt
     1561 gttattaata ttcagattcc ttatcattta cattccatat agttaatata aaacttagat
     1621 ttttagagtg caacatgaaa aaaggtcttt aatttacatt ccatatagtt aatataaaac
     1681 tatttagaca agtaaaaaac cactctgcgc aaaagagatt tacattccat atagttaata
     1741 taaaacccta ctaaacacgc tttagaggga aacaagttag atttacattc catatagtta
     1801 atataaaact aaagttactc ttaataatat agatagtctt gatgtattta cattccatat
     1861 agttaatata aaacgctgat gaaaaagagc gtcaacaact tcaaaaagaa aatttacatt
     1921 ccatatagtt aatataaaac ccacctggta aatataagtt tgaatgtttt ggtgcttgat
     1981 ttacattcca tatagttaat ataaaacccc aaaataaatt aagtatttcc aatacttata
     2041 catatacaat tctcctaaac ttgcagtgag cgagcaatag tgcaattgat aacagttatc
     2101 atacactctc aacgctttat atttcaacta ttaaacccta ctttatcaca aaaatcgctc
     2161 actgcaaaac ttttatattt ttattatatc ataaaactca tactataaaa taaaaatggg
     2221 ataattttac aaactaaaag catctacatt cttataagta tttattatat tctctattcc
     2281 aatgattcct ttttcttttt attgcatcct tcctatgtaa tcgcacctta gctcaataag
     2341 tcttttattt tcattaacaa ttttttaaat tattcaaaat catcaaattt ttcatcttat
     2401 acagttaaat caaaatacgt gttatatctc tttatctata tacttttctt ccttccattt
     2461 cttttcctct ttatacattt tcactttgtt actttacaat ctcaaggtta ttatctgcta
     2521 ttaaagtttt atctttccag gtttcacaaa aataaattga ataagtgcaa aaataacaga
     2581 tacaaaacca taactatttt cttttctata aaaaaatata taatcaacaa gaaatataat
     2641 aaatgaaaat aactatgaat aaatactctc atttagaaga aatagacaaa gatattcctt
     2701 aaaatttaag caaaatattg tttaaataaa aaaactgact gtcagttaaa tgccagtcag
     2761 ctattttata tattttatcc aaacatcact atatttgaac attttaataa ttaataatac
     2821 ttattaatat acaaacttta ctcatacaca gaatatcttt tccaaatttg ctctaaatca
     2881 tcaaaatgct gtttaatatc ttctttttcc ttcatcccaa cagatatagc aagggcattt
     2941 gaaatactaa gagctggaac tagagaatct acaaatgaag ccatattact cttaactaga
     3001 agagtgttat ctgcaagtga tgctactggc gcaaataagc tatctgttaa tgatattaca
     3061 tgggctcctt tttccttagc ataattgaca atttgataag atttcttaga ataacgaggg
     3121 aaactaatag caactattac atcttcttca tttattctta caatctgctc aaaagcatct
     3181 cccatatcca ttctaattat atgaacattg tcaagtatta tatctaagta aaaacctaaa
     3241 tattgagcta ctacaaaaga actcctcatt ccaagtatat atatttttct cgctcttaaa
     3301 agtttgttag aagcttcttt aaacgctctc tcgtcaattt cttctagtgt agaccttata
     3361 ttatctatat cactttttaa aactttgttt agtattgcaa aatcatcgga ataatcatgt
     3421 gccatttcaa ctctttgtac tgtagttagt ttatttttta tgagttcttg aagtgcagct
     3481 tgtaatttag gatatccact ataacctaat gcatttgcaa atcttacaac tgtagactca
     3541 cttactccta cagtttcacc caatttacat gcagtcataa atgcaacttt atcataatta
     3601 tttaaaatgt attgtgctat aagtttttga cccttactca atctcgtata ttgagattgt
     3661 atatttgata tcaaatgttt actgtccttc atttcatttg tttcttccat ttttttctcc
     3721 ttttagttat ataaactatt ttgttttatt aacactgtct ttttctctaa aaattttatt
     3781 ttttatctca atttcatctt agtttttatc atagcttctt aaactcataa aattctaact
     3841 tttctgtacc ttatatttca catagtattt tcgattaaat ttattatacc atctttcctt
     3901 gcatttttta atatttatcc aatatttctt ttaaattttt caatttttac tcatttaaaa
     3961 gttatctatc ttattttgca tatttagatg tatattttgt tttgagaata agctgttgta
     4021 tacttattat ttaaagtatt tttaataggt cacactatat atattaactc ttaatacaat
     4081 cttgtcaagt tttttagtat ttattatgct tttttctttt caattaaaat caattgtggt
     4141 gggttattga cttgatttaa aaacttagat tctaaaacat taaattcatc ttgatttaaa
     4201 ttattgacaa ggttatatat gcaatctttt tcttcttgac ctccatcatg ccctgtatat
     4261 atggcaatgc ttattactcc attttctttt aacatttcta aacttaactt tatagccttt
     4321 aaagttgtat ctggtttggt tataattgaa tgtttcgctc ttggaagata acccaaatta
     4381 aataaaacac atgaaacttc ttcttttata tattgattca ttttttcatg cccatcaaat
     4441 atcaatttta ctctatctgt atatccttct ttctctagtt tctttcttgt agactttatt
     4501 gcttcttctt gtatatcaaa agcatataca aagccctttt ctccaacctt ctcagctaga
     4561 taccttgtat catacccatt tcccatagta gcatcaatta ctatgtcacc ttcatttata
     4621 atattttcta aataaaattt atttatatct gtaattttag ttaaatactt tgcatatgcc
     4681 ataatactat ttctccctac tattaacttt atttcatttt ttctcaattt atttttatct
     4741 tattttattg attttaaata ttttatttca tcttctgtta ggtatctata ttctccaact
     4801 ttggtgtctt ttaatactat tttccccaca gaaatccttc ttaaatttaa aactggatga
     4861 tttatagctt tgcacatttt tcttacttgt ctatttctac cctcatgtat agttatttca
     4921 cacactgaat aatttttttc tttattttct ttagttaccc ttattttagc aggtgccgtt
     4981 gtgtaatctt ctatttttag tcctgtttcg aattgtctta tctcatcatc acttataatt
     5041 cccttcacac ttgccacata cgttttatca atttcatgtt ttggatgggt aagcttatat
     5101 gttaaatcgc catcattagt aagtattaat aatccactag tttcataatc taatctccct
     5161 attggatata ctctttccct tacatcttta agtatgtcta ttacactggg tctattgaat
     5221 tggtctttta ctgtagttat atatccctct ggtttattta atactatata tatataattt
     5281 tcacttggtt ttactttttt attatcaaat tctactatgt ctttttcttc atcaatatta
     5341 aaagataact cttttactac acttccattt acctttattc ttcctccaag aattatttct
     5401 tctgcttttc ttcttgatgc tattccacaa gaagctatat acttattaat tctcatactt
     5461 aatccttcct tatcttaaat tatgtgtgtt taatattttt atcaatgtta attttgctac
     5521 attattatac tatactagac cttaattaaa aacaagcaaa aaaattattt aattttccta
     5581 aataattgta atttcttttc acatactatt aatatgtaat agctgactaa acactatttt
     5641 aaggaagtga ttattatttc aaaggaaaat ataaaatatt atttaatagt cgtgtttatt
     5701 ggaattttat tttttaaatt tattaatact ccatcagact ttatatctag tattgaagga
     5761 ttcttaaagt tttttagccc atttttatta gctattttac ttgctttact tttaaatcca
     5821 cttgtgatgc tttttgaggt aaaattcaaa gcacatagac ttttagccat ttttttatct
     5881 tatatattta taggttttat tttagccttt gccattaggt tattaattcc atcaatagct
     5941 aatacattaa atagattgat aaatgaaatg cctatgtata ctgattatat agatagtttt
     6001 atagaaaaaa atatgtcaaa catagatttt ctaaaaactt taattcctca tatacaacat
     6061 agtttagata atttattaaa agaagcaagt aattttgttg gtaaaattcc taaaaatttc
     6121 ttaatctata cacttagtat atcttctatg ctatttaata tgacaatggg atttatacta
     6181 tctatataca ttatatatga taaagaaaaa attgcattgg gatttaagag atttctatat
     6241 tcttctactg ctagaaacaa ggcagataat attatagaat tttttaggac tactcatgat
     6301 attttctatg attatttact tggtagaata ttagactctc ttattattgg tataattgca
     6361 tttttaggat ttcaatttgt tatcagaata gaaaatgctt tatttttagc ttcaataata
     6421 ttcttaggta atataattcc ctattttggt ccttttattg gtgcaatccc tccaatagcc
     6481 atgactatat tatatagtcc acaaaaaact atatgggtca ttgcatttct atttatattg
     6541 caacaattag atggaaattt tatagaacca aaagttatgg gtaatcaagt tggtatagga
     6601 gctatatggg taatatctgc tattttggtg ggacaatctc tatttggttt cataggagtt
     6661 tttctttcag taccaatagc tgctgttgta aaaacttatg ttgataaata tatagataat
     6721 cgtttacaat agcttatctc tactgtgact ttaattttta gtttaaaatt taactgttat
     6781 ttattaaatt atttctcaaa aaatatctct tagtaagaaa aaattgctaa gagatatttt
     6841 ttataatata taatttgttc attaaattta tgcatatatt ttatacttat tgattatttg
     6901 agttttgaga atttccacca ctatctgaac caccagtagt atcttgtgga gttggtgctg
     6961 gtgttggatt actattattt ggcttttctt gattttgaga agaactgtta ttattgtcag
     7021 acttattact atctgatttt ttatcattat ttttagaact agatttatta tttttactac
     7081 ttccactata agaagatttc cttgcatctg attcaactct atacctatct tctccagccg
     7141 tatcttttgc ctttttcttt ctttgttcct ctgcttcgtt ttgttctttt tctaatttag
     7201 ttaactcttc tttcttttta gttgcctctt ctttaataac agaggaacca tttttgattt
     7261 tctctataag tttaagttcc ttgattgcac cctcataatt ttttaattct tcaaactccg
     7321 tcatatttga cattctcata gcttgcatat acatagctct tgcattctca ttagtactat
     7381 cttcttccag tgcagaggat aatgattcca tagctttttt atagttatgt ttttctaaat
     7441 atgtttttcc ttgctctact aaatttattt catctttttt attaaatgaa catcctacaa
     7501 gaaatataga taatatcgat attaataaaa ttactcttcc tctcattaaa tccctccttg
     7561 tcatatactt taaattttat cataattata aagtttttaa aatattttat atttattaca
     7621 agaaatttgt tagtaaaaca aaaaataagc agatatgcta cgaacgatac aataaaccaa
     7681 aaattaattt agagatacat atcctagcaa atctgctcat aataaacttt aatatttaat
     7741 ttttattata tacttctcca ttttatatct ctaaagctaa taagtcctca tagctttgtc
     7801 tcttacaaat tatcttatca tttccatcaa atactgatac tactgctgct cttggtattt
     7861 tattataatt tgaagacatt gaatatccat atgcaccagt tgatgttgtt ataagtatat
     7921 ctccactttt aatatccatt atgtttatat ctccaattaa tatatctcca ctctcacaac
     7981 attttccagc tattgttaca tgattcatag cctcatgatt aattttattc acaatactac
     8041 actcataact agcttggtac aacgaaggtc ttacattatc tgtcatacct ccatctacac
     8101 ttacataaac cctaacatct tttatatctt ttatagaacc tactgtatac aatgtacttc
     8161 ctgcatttgc aactattgat cttcctggtt ctattaccaa tgtgggtact tctattccaa
     8221 gctctttaca tgaagtttct gctttagtga ttatagtttc acaaaactct tttatagttt
     8281 taggtttatc tccttcgcta taataaacgc caactcctcc acctaggtca atttctgtta
     8341 gttgtatgtt gaatttttct tttatttctt ttaccaagtt catcattata tccactgtat
     8401 ctatgtatgg ttctacatca aatatttgtg aacctatatg agcgtgaaga cccacaagtt
     8461 taatattttt atattcactt aatttttcta ttgctctaaa taaatctcca tttactaaag
     8521 caaatccaaa tttagaatct atctgtccag tttttatata atcatgtgta tgagcttcta
     8581 taccaggagt tattctaaag tatatctctt gagttttacc tttatcttca cataatgctt
     8641 caagcaaatc taattcataa aaattatcca ccacaaattt acctacacct aaatctaatc
     8701 ccatgtttat ttcttcttta gtcttattgt ttccatgaaa gaaaactttt tccataggaa
     8761 aatttgattt ataagctgta tataactcgc ctcctgaaac tacatctaaa cacatttgtt
     8821 cttcatttat aagattacac atgtaaagag gtaaaaaagc tttccctgca taagcaacct
     8881 tattttttcc ctctctagcc ttaaaatatt ctacatattc tctacaattt tgtctaacta
     8941 aagcttcatc aaatacataa agtggagttt gatatttttt actaagttct aaacaactca
     9001 ctcctccaat atataattca tcattattta cagtcatagt tccatgaagt ttcataatag
     9061 cctccccaaa ttttatatat taaatacttc tgctattttt tctatagcta cttgcttatc
     9121 atcagaattt atagcacaag ttatacgaat ctctgatgta gttataagtt ttatcattat
     9181 accattttca ttgaatatct taaatacttt agcagcaact ccagaagtat ttttcatacc
     9241 aagacctact aaagaaaatt tagtgatatt attatcaatc actacatgtt cttcatctgt
     9301 atatttagat actatctttt tacattctcc caactcttct tttggaactg taaatgatac
     9361 acttatctta tctaaaatag gtgcagtttg gcttatcata tctacactta tacctatagt
     9421 tgctatatct tcaaataagc tagatatatt ttcagcttta aaatctttta ttgttataga
     9481 actgtcatca tcactagtag ctaatcctgt tattacttta tcctctaatt tcatgtcttt
     9541 tccccctctt atatatgttc ctttttctgt accacatgta cgtccgacat agatttcaac
     9601 accatatttt tgtgcaagct ctatacttct tgaatgcatt acttgtgctc ctaaactcgc
     9661 taactcaagc atttcttcat attcaatctc atcaagttta ctagcctttg aatattttct
     9721 tgggtctgta aaataaattc catcaacatc tgtatatatc tcacattttc catttaattt
     9781 aaccgcaaga gctacagcag atgtatctga accacctctt ccaagagtgg ttacatcacc
     9841 atcttcgttg attccctgaa atccagttac tatcacgaca tttccttcat ctaaactatt
     9901 tttaattctg tttacgttta tatcatcaat ttgagacttt ccatgaagac cactcgtttt
     9961 tatatttaac tggtaagcat tataacttat agctttacat ccaagtgcat ttaatgctat
    10021 tgataataaa gatgctgaaa tcatttcacc agtagacatt aaagcatcta attccctctt
    10081 actaggttca ttactcaatt cctttgctaa agttatatat tcatcagtgg attttcccat
    10141 ggcagacaca actattacca tttgtggatt ttcttttctt cgttctatta tattttctgc
    10201 tattgatttt attttttcag tatctgctac tgaacttcca ccatattttt gaacaattat
    10261 actcatgctt tctccccctc aagctaaatt ttgataaata aaaacgccca aaggtatact
    10321 ttggacgtgt aaatttcata gaccaagtaa ccttgtagct caccacttta aaagtgacag
    10381 ccttatgcat attctgcata agtccagaaa atttgtccgt caacaaaatt tcttcggcta
    10441 tatacccttt catcaattat caacgcttac cagctcctat tgaatactat tgaatacagc
    10501 tacctctacc ctaggctcat tatttttaat taatttatta atatagtttt atcatattct
    10561 atataatatt gcaacaatta ttttacaaaa tgtggcaaat cgatagtttt cttttctaaa
    10621 gttagcttat atacaattaa atagtttatt taatcctaaa tctataaaat ttatgctata
    10681 ctcattatca tatctctcta aatcatttaa ttcggataga ttatctggtg taactaccaa
    10741 aacgtttggt actttaaaaa atggaattgt agatttatct atttttttag aaagattttc
    10801 gtatttagaa atgtctattt cattatttaa gtcaacatca actaaaactt gctttatatt
    10861 accaaattgg tctgtatatt caataaataa atcgtatttc acatcatcta cactaacatt
    10921 tcggtaaaat ctttttacat cacaacctgc agtattgatt ctaacaacta attctgatcc
    10981 tactagagcc tttattaaat catctccctt aaaaagttta ctaccgttta aatagtatac
    11041 ataagagagg tctttataat tataataatc cacatcacaa ggatattttt ttagtgtata
    11101 ttcactacac atttttctca aagttttttt aaaatttttt ttattgtatc ttttttggtc
    11161 tccataaatt tttctaaatt gagactctgt aacacatcta accctgctaa ttaaaagtaa
    11221 tatctctttt tccatattcg tcatagtaca ttctccttga agtattattt gcaaagttgt
    11281 actaatatta tattaatttt atgacattaa atttacaaag aattaaaata aaatagctaa
    11341 tttttataga attaaattct ataaaaatta gctatcacta tgttaaatac tattatcaat
    11401 ctttttattt aaattataca ttaccttgat tttcagaatc tttggaatct gtattttcat
    11461 cagttcctgt tttttctttt gcttttttat cttcaaagtt tgcaatattt aggttatcaa
    11521 attctgacat ttctattaca ccagattctt cttttgaata cgatattttg actttatcac
    11581 caacctgagt caatggcaat tcgctagata cttttgaagt agccctaaat attatatctt
    11641 tatttgaatc taaagttaca taataattag tgttaccacc tcgcacatct gggcttattc
    11701 ttgtcactgt gccttctaat acctcatctt ttaaatcatt atcaagtttt aaatcattac
    11761 ccttagattc taatgcatcc ttatagtttc ttaatgcttc attcttatct tcacctattc
    11821 caagtacatt ataatcttca acagatacaa atgcaactct cttaacaaga ccagccttat
    11881 cttttaatga tgatacatat gttggctgac ccaatatatt atacattaca gggaacgtcg
    11941 cttcataatt tttttcttga acattacctt cagctgattt cattgcagcc gtttcagtag
    12001 ctcctggttg tttatataat ctagcttctt ttgttctaga atcaactaac ataaatccaa
    12061 ttgtagattc atctccacct gaagatgtta tacctgtata ccaataagac ctgttatcat
    12121 tgccataaac taatgatgta ccttctgttg gtactaaaac tcccttttct gatataacag
    12181 agtttaaaaa tccattaaca taaacacccc agtctttaat ctggtctgtt acaaagcttt
    12241 gtggttgtat tctatccacc catttaggcg tgtttttaac atcataaaca tttatttttc
    12301 cagtttctgc atcaacagtg gctattcctg ttgcatttgc tcctccatag ccaattttat
    12361 gttcatataa acttacaacc caataaggtc ttccttcatc atttatttca agagtgaaat
    12421 ctgtcatacc aacatttaca ataccatgta tgtataaatg tctttgtaaa tcctgatgta
    12481 agtaggcttc tggttggtaa actatcttta ttggctttcc atctatttct tgaactaatt
    12541 ggacgtcttg aggattacta gctgatactt ttacataacc acttgtacca cttagagatg
    12601 ttatccattt aattatgtct ctgtgaacaa gaggcgctac ccagtacaac tctccatcta
    12661 cattttgtat atgaaatttt cctaatttgg atacactccc tattgcagga acttctccaa
    12721 gctttttgtc acctaatttc attgccatat cttcatctac aagacgaatg tcatttacac
    12781 tgacagggct aatgtcatca gtaaatttac tttcagttac ttttcccaat agttctctat
    12841 atgattttgc atgaagtaca ggtgtagaag ttataaaggg aactactaca atatacacaa
    12901 gcaaagctat tgctattgaa aaattatatt tagccatttt agatgctctt tcatttctat
    12961 ctaacatcat atctagtatt cctgctacaa caaagaaaat tattcctact gaaaataaag
    13021 atgtaaaatc taatcttagt acaggtaatt taataaatgc ataaattatt acaattatta
    13081 atgcaactcc atatgtttta agatatcgtt tcatttaata ctcctctcta aaaaatgtta
    13141 ttattactat tatatccata tatttacaaa aaataaatat ttatcatatt tattatatat
    13201 taagatatct ttatatattt atagttctgc ttattcaact atatttaaca ttatacgtgt
    13261 atgtataaat actttttata cactgtttgt actatccatc ccgcacctaa aggagtggga
    13321 tttttgcact attctgtatt atatatatcc aaaaatatta taaaaataca aatattagca
    13381 gaaatttaaa aactgaagaa agtattctaa ctaagattta caaattctta atcctatcga
    13441 agttaaacta ctatcttcag cctaattatc tagcaatatt tttttatatg aaaaactcta
    13501 aatcaagttt ttaaatgttt gcgatttttg atacataaga atgttgtcta gaattatatt
    13561 ctgataataa accttcatac tctaaggtta agccagataa attataaaaa tcatttggcg
    13621 ttacttcaaa tgcttgttta tttaaaatgt ctatctcact tttttctaac atgtcagcaa
    13681 caaaatttga acatacatat ttatattcag attgtctctg aatattcatt agtaaatata
    13741 ttaatgccat tgtatcgtaa tagtaatctt ctctataatc actaattaat tttatatttt
    13801 tatatagatt ttcatattgt aaattatcta cttctaacga atatacccta cacacagtgt
    13861 tttttcttat agcatataat ccttgattta tattttcttc aacaaatcct ccaataaatg
    13921 gattccttgg atttagcctt ccaaaagaat acattggttt tagattttca tttaatgata
    13981 ttgacacatg tgtatacagc tttttactta tattccttat taagtaagat aatatagttc
    14041 ctgtatatgt tgttacaata tagactttat tcatcatttc cccccaatct agtaactaaa
    14101 taatattata cacttacaat ttaatatcat aagatattct aacataataa atgtggtttt
    14161 acaaattttg attttaaggt actttaatat tatataatat aataaaaata taaatataag
    14221 ttttctatat ttttaaccaa cagatgtttt cttcaaatta atcagatgat ttagtatatc
    14281 ttgaaccttt ccagagtcct ttaaatatcc taatgcccca tttatttgtg attttaaatc
    14341 tatttctact tccatatcca aatcaacatt tccttttttt atagatatat ttttaagtgt
    14401 tattggataa agataacttt tttccaaagt aatcttattt ccattaacct caaatggaac
    14461 ttttttattg tagttactta atattttttc tagcatttta tcacttagtt taaattttcc
    14521 aattttcaca ttctcaagac ttacaactaa gtttccatcc tttaaagtag gtctcaattc
    14581 aagttcatat tgactattta ccaaaccaaa taccttatat ggtcctgcta cctttatttt
    14641 accatttttc atttcaacaa aattattttc aagttcatta attccatgct tctgcatcaa
    14701 tgtatatatt aaatttttaa actcttcctc tgaaacagat atttttccaa ccattctaag
    14761 tggatttttt actacctcca tagaatttat atatgagcct tttgataaat attcttcatc
    14821 tatgatttta ttattttgtg aactaacatc atatctttct tttggtgtca agatatatac
    14881 aacaattcct acaagtacta aaacaataac aattaaactt aacaaaatct ttgcaacctt
    14941 tcccataatc gtctccttta caaatctgtc atcagattta aaataaattt agataactaa
    15001 cttcacataa tattcttaac aatatacata ctataataca gtcttttaaa aattaattca
    15061 accaatctaa tgttttgtaa ataaactgta aaaacatgtt tttagtataa aagttaatat
    15121 tttgcataat actcttaatt acacctttat ttttttctaa tcttggatta aaattaatat
    15181 tatgcaatac agtgtagtgt tctgtattat aatcaaatta aataggaggt atagaatatg
    15241 tcaaatgaaa cgaactcatc taactttata aagaatatta ttataaacga cttagaaaca
    15301 ggaaaacatg acagtatcat aactcgtttt ccacctgagc caaacggata tttacatatt
    15361 ggtcatgcaa aaagtatatg tcttaatttt ggattagcta aggagtttaa tggaaaagct
    15421 aatttaagat ttgatgatac aaacccatta aaagaggatg ttgaatatgt agagtccata
    15481 aaagaagatg taaagtggtt aggatttgat tggaacgagt taaattttgc atctaactac
    15541 tttgatgaaa tgtataagag agctttaata ctaataaaaa agggtaaagc ttatgtatgt
    15601 gatttgacac aagaggaaat gagagaatat cgtggtactc taactgaacc aggaaaagaa
    15661 agtcctcata gaaacagaac tattgaagaa aaccttgatt tatttgaaag aatgaaaaat
    15721 ggtgaattta aagatggtga aaaaacatta agagccaaaa tagatatgtc ttctccaaat
    15781 ataaacctta gagacccaat tatatataga atatctcatt caacacacca taatacagga
    15841 gataaatggt gcatatatcc tatgtatgct tttgctcatc caattgaaga tgcaattgaa
    15901 ggaataactc actctatatg tactttagag tttgaagacc aaagacctct ttatgattgg
    15961 tttgttaaag aatgtgagat ggaaaatata cctagacaaa tagaatttgc tagacttaat
    16021 ataaataata ctgtcatgag taaaaggaaa ttaaaacaac ttgtagatga gggtattgtt
    16081 gatggttggg atgaccctcg tgttcctaca atatccggaa tcagacgtaa gggatatact
    16141 gcagaagcat tgcgcaattt ctgtagtgaa ataggggtat caaaagttaa ctctacggta
    16201 gatagtcaaa tgcttgatta tttcctaaga gaaaacttac aaccaaaggc tcctcttact
    16261 atgggagttt taagaccttt aaaacttatt ataactaatt acccagaaga taagattgag
    16321 atgcttgaaa tagagaataa tgctaaagat gaatctcaag gaaagagatt agttcctttc
    16381 tctagagaat tgtatataga acaagatgat tttatggaag aacctgttaa gaaatatttt
    16441 agatttttcc caggaaatga agtccgttta aaaggtgctt attttgtaaa atgtactgat
    16501 gtaattaagg atgaaaatgg aaatgtagtt gaaatacatg gtacttatga ccctgaaact
    16561 aagagtggct ctggctttac aggacgtaag gtaaaatcta caatacattg ggttgatgct
    16621 aaatcagcta tcccatgtga atttagatta tttgaaccat taattcttga tgatatacct
    16681 gaaaatgaag gtaaacattt cttagaacaa ataaatccta attcattgga aatattacaa
    16741 ggttttgttg aacctacaca gataaaagat gctaaacctt ttgataaatt ccaatttgtt
    16801 agaaatggat tctttagtat agataataaa tatacaactg atgaaaaatt tgtatttaat
    16861 agaatagttc ctctaaaaag ttcttttaaa ccaggtaaat aatcttacaa tcaaaaaatg
    16921 aagacttaaa atataaatta cttctactat aaataaactt agttagaagt aattttttat
    16981 ttgagtcttc atcatttact ttaaatgatt aacttttttc actacagatt cccatgactt
    17041 ctattccatc ttttatgcat tctatgttaa gaggaaaatc tggtcctttt tctccgtcaa
    17101 tatctgttat aagtgaatct tcacattcta tatgcacctt tttagtttta aagtatagta
    17161 tttcatttcc attatattga tctaaataat cacctctaag cacacttatc aaaactggta
    17221 ttgattttgg tattggcatt cctttaaaaa taattacatc caaatatcca tcatctattt
    17281 cagccttata tgctagattt atatttcctg ctgttttgcc attaaaaatc aacataagat
    17341 acatatctcc atcataatac atttcctcag aagatacttt tatcttaaat ttcctcatat
    17401 acaatgcttc ttctattccc tttatataat aagaaattct tccaaatgaa ttttttaagt
    17461 ctgtgtttat cctttgtgat acatccgtaa acataccagc acttgcaaca tttataaagt
    17521 acttattatt gactttccct atgtctattt tctttggttt tgaatttatg atttcattta
    17581 tagcttcttt tatatcaaat gaaatttgaa gagcatttga aaaatcattt gcagttcctg
    17641 taggcaaaat tccaattgga atttttatgt ctaattcttt cattgcattt actactaaat
    17701 ctacagtacc atcaccacca gctatcaata tgtgttcata agaactatca acttccttaa
    17761 atgcattctt tacaggttct ttaatagtta atctataggg tatcaataaa taattttttt
    17821 cttgatataa ttctatgata gtgtctaaat ttgataatat ctttttttca cctgaattag
    17881 gattgtatat aagttttact tttttcatta tttctcctta ctcattttat tcttttagta
    17941 tccaaaatct tcatttaaaa aaatcacatg tattctttct ggtgttaatt gtctttttat
    18001 aagtgtataa ctacagcata ttcttcctgg acttgaacag tctatacata taccagtagt
    18061 cttacatcca gtatttgtac ttaatctttt acaattggct ggagcagcta ttgtttttgt
    18121 tctttttata gcttcatcta catctttaac tattttattt actcctgcta taactattac
    18181 tttatcaggt ccatatatca tagcagcaac cctattccca ttcccatcta aattgtaaag
    18241 ttctccatcc tctgttatcg cattagtact tgtaaaataa gcatctgcag aaaatgactt
    18301 tctatatatt tctttcattt cttctggttt gatatctttt gcatatctat caagaaaatt
    18361 atatttttct cttataaaat caatcactcc tgtttcaaat aaggtcatag aacctccaca
    18421 tcctactaga gaacctgctt taactatttc ttcaattttt ttaagaagct cttctttgtt
    18481 acttactaaa taccctttca tattattgtt ttccaaagat tttattgttt tttctattct
    18541 tttttcatta agccaattta aattttgatc cattttaact tcccccttat ttttaatatg
    18601 tacttaatta tttataaact ttattttctt tataagttta ttttagtaca tctaaaaaaa
    18661 atacaatata aaacttaaat agttttacat aattttttcc aaaataactt actcaatcaa
    18721 aaaaggaaag tagatgtact attctacttt ccctatattg tatgtagtat tatatattgc
    18781 ctaaaatcta tatattaatt atatgtttca tattatatga atatcacagt taattaaaat
    18841 gtaatactat cttcttttta aacatataaa ttgtattatt aaatttaata tatccaactt
    18901 caataaatct tattaatttg atgaagaaga tgctatagct gcacttgttg ctgccattac
    18961 acatgcaatt tgaatagcaa caacaatatt taaaaatata ttattagtta tagagcctat
    19021 attattctca tttcgtatca aatctgcata aattgaagct acaatagcag aactaatcat
    19081 tactctatta ctttttccta aagaccaatt tccaaagcct ttttcttctt taagtttatt
    19141 tgaaacattt tttatctctt ctattatttc atcttctcta taatctaata atgaaattgc
    19201 tcctattatt ggatatccat aataattcat tttacaatca ttttctttaa atgctttttt
    19261 aagttcaata gtctttttac atttgagcat agttctatca tcactaaata aaagaatttg
    19321 ggataatgct tgtaaatcat ttcctttata aaaaccattt tcatttagaa attcataaca
    19381 tttttctatg tattctaaat cttcatctaa attttttgaa tgaattgcaa taagtgctat
    19441 attacaatag tcatcactag aagttagaaa tggatgtttt ttcctcatgt aatcatatat
    19501 tattttcatt tttgatatac atgtaagata atccattttc tctctatttt cataaactat
    19561 tgatgctgta agtggtaaat atgtatcgcc ccaaaacttt tcacttttaa gttctttgta
    19621 tatatctaaa atcatcttaa aactactttc tggatttgat tcaaacgaaa gtagtgttgc
    19681 catataaaac atattatatc ctctaaaatt agagaataat ccagtatttt ttttaataag
    19741 ctctattcgt tcttccacta atctaggact tattctcttg ttcttaaggg taaatgcaag
    19801 tgcacaacta tgttgtatca atcccatttg ccaagaacct ttaacttcat ttaatgcttt
    19861 gtaattttct attaataagt ttacttttcc atttataaac gattgttcca tattgccctc
    19921 ctagtaatat atctcattgt actaatagtt ttatatttat tatatatata ttaaatacta
    19981 caatcaatac atattacttc aaagaacatc attcttcaac aataatgtag ccttttttgt
    20041 aatgtcttcc catattattg actctatcat agtactctga ttcactttca ttactccatt
    20101 tgggcacaaa tacatctatt gcattagaat tactaaaata tttactagac cccctatcca
    20161 taacagtttt taattttcca tctacaataa tcttagttcc aaaacttaag tgattgtttg
    20221 ctacaaaacc atcttctaaa tcatctcctg tagcagttat cgctccatat ccattttctt
    20281 gattaagact tgtgtaataa ctaacttcaa aagttatagg tactaacttc tttattttaa
    20341 tacctaactg attttgtagt ttctcctttt gtactttcaa cttatactca tcactatttt
    20401 tataatcttc cataccttga ttatacttgg aattaaagct tttacctgac tttactatag
    20461 tatctatatt tactatagat tctttacaat gatctgcatc tatttttgtt tctccattta
    20521 ccaataatgg agttaaacat acactacata caacacatgt aatcaatgct tctttaagct
    20581 tcataaattc cccccttatt attttgatat attagcatat ttaatattat atgtctacat
    20641 ttagatttaa ataccacacc catttgcttt aagaagttgt cttatattta attcacctta
    20701 aacacttata aatcctctat cttagaatca tatagaataa agcttttatc tttaaatatt
    20761 ttttattaat cttttaaaat ctaaaactac tttatctaat tttctctttt ttagaattta
    20821 agaattaaaa ttctataatt ttaaaacata ttgaatttat ttttttgaga tgatataatt
    20881 ttcttaagag gtggttgata tgataaaaaa tacaaaacaa attacagtag aagttaatga
    20941 aattttatta caagaaatgt ctaagataac taatgacgaa aataatattt tgacagattt
    21001 gataaatgaa tcaattgtaa attatattct tgaaaacaaa aaacataata ttgaaaataa
    21061 agatttagaa gaagtgatta aattttacaa aaaagaaaat actgaaaaac ctaaaagtgc
    21121 agcaagagct atagctgaag caaaaactca tactctgtat taatcaaata aaaaagttcc
    21181 taaaaactag gaactttttt atattaataa ttattaatat atctcaatat ttatctcaaa
    21241 tctatcaata taagcatgtt taatacaatc tattgtatac ttatcttacc ctagaaataa
    21301 tcaaaacaaa aataaaatta tgccctttat atctaaaaca attaatttac atgattaata
    21361 tacaaaaatt ctgactttta aattcgtata caatatcaaa ctaaaataat attaatgaca
    21421 aacccaatat aaattaaatt caaacaggtt tgattttaca tcaaacttat tgtattaata
    21481 ttaaataaca agacaaaaat atttataaaa atcacaaatt ttacataaaa ctttatattt
    21541 gattcattta actaattatt gtaaaaacta tttattttat gataattaac tacaccaaat
    21601 tattcatctt ttttaatatc cttaacccat ttttcaacag agttcaattc ttcttcattt
    21661 accccctttt tgaacaagga tttcataaaa ctctcaatag aacttccatg catattatct
    21721 aaaaaagttt ttgtttcaaa atgtaaatac tcttcttctc caataagaac tgtataatgt
    21781 gtatgtctat caatttttct tgcacttaag aattgcttat ttactaatct tgataaaagt
    21841 gttagtgttg tagtttgctt ccaattatat agtttttcca tagcttcaat gacatctttt
    21901 gatgctactg taaaatctga actccaaata aatttcatga cttttaattc tgctggtggt
    21961 atcttagtaa tagtcatatg attttacact cctctcaaac ttctacataa ttaaatacta
    22021 tattaataga cttttcgagt caaaggtttt atttaaaata tctcatccaa atttattaat
    22081 acaaaaaatt tatattttta ttcaaaatta aacacacaat ctattaatat aaagataaat
    22141 atctattcta taagtataac agattatcaa attaaaattc taatatttga cataatatat
    22201 acatttttta aataaatata tacaatgtat taaaatgtat gcaactctat ttcaaaatta
    22261 ttttgtgata tatatatgtt ataataaatg tataaaattt tgattggtgg gttatatatg
    22321 aatgcattta ttagaaaaag aaataaaaac tatgttgttt acctggaatt tgaagatgaa
    22381 aagacaggta aaagaaaaca aaaaaatatg ggtacttttg ataaaaaaag ggaagcaagt
    22441 aaaagactaa atgaggtaaa agaaagctta tataatgatg gttttttagt accaagcgaa
    22501 attacagtgt cagaatttct attagatttt ctaaataaat atagtgaaaa tatatcttta
    22561 gctacatata ataattatgt tagtatttgt aagaattaca ttaatccatc tattggaaaa
    22621 tataaaatac aagacttaca tccggttcac atacaaaact acatagataa actatcttat
    22681 aaactaaatc ctcaatcaat taagattcat ataaatatat tgaaacttgc aataaaaaga
    22741 gcttatagac tcaaattaat aaaagaaaat gttatggata gtatagaggc tccaagatat
    22801 aaaaaattta aaaatgagat ttatgataga gaacaaatga taaaattatt agatttagca
    22861 aaaaatacag aaatggaatt acctattaat ttggctattg gattaggtct tagaatttca
    22921 gaagttttag gtctaacttg ggataatatt gattttgaag aaaatacaat aacagtaaat
    22981 aagataactt cccgaattaa tggttctgtt attctcaaag agccaaagac agaaagctct
    23041 gttagaaaaa tatcttctcc caaagaatta atgtctttat taaaagaata taaaataaaa
    23101 cagaataaaa atttattaaa atcttccatt agaaataata ataatttgtt gtttttcaat
    23161 aagaaatgtg aacctattgc tgaagatgtt atgagtaaaa aatttaaaag atttttagaa
    23221 aagaatgaat tacctcatat tagattccat gatttaagac actcacatgt tactttactt
    23281 ataaattcta aagtacctat aaaggttata tccgagagag taggacactc aaatataagt
    23341 actactctaa gcgtttattc tcatgtactt aaagaaatgg ataaagaggc ttctgacaaa
    23401 atttctgaaa gtttatttaa tgctaattaa tattaattta tcttagcttt aaattagctt
    23461 agcttattga gatagattca ttaatattaa atgtatctat ctcaaattaa taactactta
    23521 aataatctgt tttattttaa tttatcgcct cagttattat acttttttac gaaaaattat
    23581 actttacaat aaaaaagata ttcccttata gaaaatatct ttttaaatac tatattatta
    23641 tatacttgct aacattggaa tatctaacat cttcttcatt acatcatcta ctttttttgc
    23701 tttatattca ttgtatccaa ttaaatatcc tttttcatat gaacttttta aaatctcttt
    23761 ttcatataaa ttatcagatt tggcttctgt gacacttaaa ttataaagcc ctttacaccc
    23821 atcttcataa cctttattat accctttttc tgcaaaatag tttttatgat ttttttcgtt
    23881 atgtactgaa gatattgtta ctccagaatt ttcttttgta ctagcataag gacaaaaacc
    23941 tttattgtgt tgatgtgctg aatgtcctcc acaataataa cgtgttaact ctgatttatt
    24001 tttattatga tgacctcctg atgaattatg ccctcctgag tgagcatatg aaaaattccc
    24061 tacactaatc atagctaaac taattactac agctactaac cttttcttaa ccataatcta
    24121 ttccccccaa aatgttatat atttttatta tatcatctac tatattatat ttaaattctt
    24181 aatatacaaa atattactct attctgctaa taaacatgtt taaacaacat aaaaaacctc
    24241 ctaatatttg attaaaaatt tacacactca tgcatagcat gtctcaaata ttaaaaggtt
    24301 ttatactaat aattatttat cttttccata cattttagct aattggtctt gtatctcttt
    24361 actttctaaa tactcatcaa agtccatttt tctatccatt attccatttg gagttatctc
    24421 tataattcta tttgctatag tagatataaa ttcatggtca tgagaagtaa ataaaagcac
    24481 tcctgggaac ttttcaagtc ctttatttac agaagttata ctttctaggt ctaagtggtt
    24541 agtagggtca tctaatacaa gtacatttgc atttgaaagc attagcttac taagcataca
    24601 tctaaccttt tctcctccag atataacttg agcttctttt aatgcttctt cacctgagaa
    24661 taacattcta cctaaaaatc ctcttataaa actttcactt ttttcttcag agaactgtct
    24721 aagccagtca accaaagaat attctactcc gtcaaaaaac ttattatgat tttttggaag
    24781 ataatcttga gaagtagtta ctccccattt atactctcca ctgtctggtt caagttctcc
    24841 cataactatg ttgaaaagtg cagtagtagc tatttcatct cccataaata ctactttatc
    24901 atctctatcc aatctaaatg acacattatc aagaacctta accccatcta tagttttagt
    24961 taaattgtgt acttctaaaa cttcattccc aatttctctt gcaggtgtaa atcctacata
    25021 tggatatctt cttcttgatg gttgtatatc ttcaacttct aatttctcta actgtttctt
    25081 tcttgatgtt gcttgtttag cctttgaagc attagaacta aatctagcta taaattcctt
    25141 taattgagct atcttttctt cagtcttttt attttggtcc ttagccaatt gtaaagccaa
    25201 ttgacttgac tcataccaga aatcatagtt tccaacgtac atttgtattt taccaaaatc
    25261 tacgtcaact atattagtac atatttgatt taggaaatgt ctatcatgtg atactattat
    25321 aactatagat tcttctaaat ccattataaa attatttaac cagtttattg atttaaaatc
    25381 taagtggttt gtaggttcgt ccattaatag tatttcaggc ttaccaaaaa gtgattgtgc
    25441 taacaatacc ttaacctttt ccccaccaac tagttccttc atttgtttgt aatgcatatc
    25501 tttcgttatg ccaaggccca taagtatttt ctcagcatta gtttctgcat cccatccgtc
    25561 aagctctgca aactcacctt caagttcggc agcttttatc ccatcttctt cattaaagtc
    25621 ttccttcata tacaatgcat ctttttcttt cattatgttc caaagtctct catgacctct
    25681 tataacaaca tttaaaacag tttcttcttc atattcaaag tggtcctgtt ttaaaactga
    25741 cattctttct ttatcagtta tagatatact acctgtatta ggctctattt ctcctgataa
    25801 tatttttaaa aaagtagatt ttcccgcacc attagctcct attattccat agcaattacc
    25861 tttagtgaat tttaagttaa catctttaaa tagttcctta tcaccaaatc taagtccaac
    25921 acctgtaact tgtaacatta atatattttc ctttcttttt gctcataatt tattttatca
    25981 taaaactttg ataacatttt tattatatat tatcacgtaa atagtatatt ttttatacta
    26041 aatttgtgaa atatttatta aaataatact aaaaattgta aatttataaa aattaacaat
    26101 ctcttatatt atacttataa agaaatcaaa ttgcaacttt attcattctt ttacacattg
    26161 aaaataagtt attataattt ctaaataaat atgcttttct taaaacatca ttctagctgt
    26221 tttgtttcat atttaaaaag agtacttaat ttgtaaatac tcttttttaa taactttata
    26281 ttttagcaaa attacatgca ggataagtac atacatcaca gttcatacaa aggccaccat
    26341 gaccatatct agctatatca tacttatcaa gcttttcatc aattaaaacc ctagataaaa
    26401 ctatatcaaa tactgtagtt ttgtgataca ttgcgcatcc tggtataccc ataattggaa
    26461 cttctccata ataagcaagt aaaaacatag ctcctggtaa tattggtgaa ccatatgtca
    26521 ctaaatcagc tccagtttct tttatagctg ttggtgtaac atcatctggg tctacactca
    26581 tcccaccagt acaacagata agttctgctc cttgacttat aaattctttt attgcactct
    26641 ttataatctc tttatcatct gggcatatag tctgtcctat aacttcacaa ccaaagcctt
    26701 ttactttttc ttctattacg ggtccaaatt tatcaacaat tctgctataa aacacttcat
    26761 ttcctgtagt aacaattcct actttcttag gtttaaaagg tactacattt actatttttc
    26821 tattaccaac tacttttttt gcatcttcta attttttctc atctataacc aaaggtatca
    26881 ctcttgttcc agctactttt aaacctttat ttactataaa attattatga agagtagcca
    26941 tcattatttc tccaacacaa ttcaaatcaa aaagtgcatc aacatctatc tttaatagac
    27001 catcacatgc tgctataaaa tctattttcc cttcttttat ttcactaaaa tctaaatttt
    27061 ctccagcagt tagttctttt aaaaacaatg cgccttcatt ttcatgaacc attccttctg
    27121 acttttccca tacatacaaa ttatctttac caagagataa taacactgga atatcttctt
    27181 cttttactat gtggcctttt ttaaattttc ttcctttaaa ttctccaggt attatttgag
    27241 ttatgtcgtg acataaaact tgtccaacag catctatagt ttttattagc ttcataattt
    27301 acccccaatt ctctcatagt ccatttttgt atcgatatca aataacattt ctttaggaac
    27361 ttctacatac tttatttttg atgaattttt taaaatggtt ttaccttttt catcttcttt
    27421 aagtgccaac aattcttctt tcttgctata tgggaatatg actggatttc cacacatatc
    27481 tttgtatctt ggaattacta taaaatctct atctaattta aaagtattta taattttatc
    27541 tatatataag ttatcaataa atggctggtc tcctacaaag aacatataac catcacattt
    27601 tgagctattt aaaactccta attttataga ttcactttga cctatatttg catttttatt
    27661 atctatatat tcaaaaccat atttattaca caataacaaa acctcttcat attgacttac
    27721 tactacaact tgatgaaaat caatcttgct aacttctcta aatacatgtt ctattataga
    27781 acagccttta taatttaata aaagtttatt tgctcccatt cttctggcaa agcctgatgc
    27841 cattatcact gcatttatca tatatatcat ccttggtatt tataatatat tgatagttct
    27901 atgattttta taatatctct attgatatta tctatatata taatatcata taaactattt
    27961 taatacttca atatcttaca
//
LOCUS       DAGSRM010000042        27852 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.042, whole genome shotgun sequence.
ACCESSION   DAGSRM010000042 DAGSRM010000000
VERSION     DAGSRM010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27852
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.042"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            95..1396
                     /locus_tag="MJE33_002024"
     CDS             95..1396
                     /locus_tag="MJE33_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBY9647958.1"
                     /translation="MEKFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKAILDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVEPVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISVALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSAHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGVIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            1533..2627
                     /locus_tag="MJE33_002025"
     CDS             1533..2627
                     /locus_tag="MJE33_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBY9647959.1"
                     /translation="MERRLGISVYPEHSNMEDDKAYIKMASKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDILQISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATATKHLFATG
                     LIDDVIVGNAYASEDELKRMSEVNEYQVVFNVVAEEKTSEVEKIIMVNEQHFRRGDIT
                     NQVARSTEVRKKYAKENFPKHDNKNLFKRGDVVIGNNDFGKYKGELQLILQDCTDERK
                     NKVGRIKEDEIILLDFIEPWSKFKIILEGE"
     gene            2629..3762
                     /locus_tag="MJE33_002026"
     CDS             2629..3762
                     /locus_tag="MJE33_002026"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HBY9647960.1"
                     /translation="MKIDILIKCGKTVDKSIIDIAILDDKIIEVKNQIDDSKYQAKQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDIGKFY
                     EDTKKYKTNVYALINIAKQGITSQDELSSMMNIDEYELKRAVKKYKDFVVGIKARMSK
                     SVVISNDVEPLKVAKRIKNELNLPMMVHFGSSPPTIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNEVKKEIIEARERGIILDVGHGTESFSMDIAMKSKDAGIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIINKVTKNAADAISLKNKGLIKEGYDADLTIFDV
                     VNEEKELQDSLNKSVITSTSIKPRAVVVNGEYLNIGKSIEENE"
     gene            3780..4889
                     /locus_tag="MJE33_002027"
     CDS             3780..4889
                     /locus_tag="MJE33_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HBY9647961.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEVIEAIGEGCKNFF
                     IIEELINKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKDDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDVVQVAKEYNLPLIVDAAAEEDLNVYYELGADLVIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTIKGVSASITRDSAGREILRGEIDFNEDIINKSTQQIINELRSGEIAIYT
                     RDYKANEGKIEIDIRSVTGSDLDTIYNRIKNIVES"
     gene            4954..5694
                     /locus_tag="MJE33_002028"
     CDS             4954..5694
                     /locus_tag="MJE33_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HBY9647962.1"
                     /translation="MNCIPQYYKNRVCLNVLAGSIKNAKEVYDACDGHVLIGVLSKNY
                     STVEAAIEDMKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFTGVATS
                     RALLGQNDSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVPIETAIAMMKDMGGSSIK
                     FFPMGGLKTKDEYIEVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKVIPHVY
                     TSIIDKETGETKVEDIGVLYKIMKELLG"
     gene            5699..6691
                     /locus_tag="MJE33_002029"
     CDS             5699..6691
                     /locus_tag="MJE33_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBY9647963.1"
                     /translation="MNILAFGEIMMRLSVPDYKFLTQTNELNYIITGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKALSANIRKLGVKDDFITYGGNHIGVYFLENGYGERPSEVT
                     YLNRLNSSFCESKLQDYDIDKCLEGMDIVHICGIAMQLTDSVKEIALELARKSYEKGI
                     KVFFDFNFRQSLNPERTYDDLVNDYKKILPYCHGLFASYRDINKILNIDSLNIKSSSE
                     LIKKEYDIGIVAGTMRRFDDMNNRFIKGYISNDDGYFESREYKLEIYDRVGAGDGYVA
                     GIIYSYLENMKSEDIVEFGVASSVLAHTTYGDNSLVSTGHVLRLMNNEKIDILR"
     gene            complement(6879..7760)
                     /locus_tag="MJE33_002030"
     CDS             complement(6879..7760)
                     /locus_tag="MJE33_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBY9647964.1"
                     /translation="MNTKQLEYFISVAEKLSFTKTAEKFYISQTAVTQQIKALEEQIN
                     VTLFTRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVSSNMGISILPSFCVTDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
     gene            8034..9422
                     /locus_tag="MJE33_002031"
     CDS             8034..9422
                     /locus_tag="MJE33_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647965.1"
                     /translation="MEDKFYAKGNGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLIYRVIDIINPTNPVEIGKWWRPDQYADGYPNRTFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLVVLDVEDLTRPRCLGELPFTPAFSSRL
                     AGARTHTALPLPGRDLVVVQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKPGPFGPHNLHEPMDNKPWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFPGPRLAVTEDLIVDDRGYIIIDAL
                     DDGFYILKMKED"
     gene            9450..10403
                     /locus_tag="MJE33_002032"
     CDS             9450..10403
                     /locus_tag="MJE33_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBY9647966.1"
                     /translation="MKILGISFGTKNGNNDTMCKVALKGAKEAGAEVEFIQMSSINIK
                     HCTGCCACVKTLLSGKGSMCVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHTIM
                     DRFGPRADRGNNIIGTKVAEVIGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHAML
                     ALIPMWKVIDNAWFPWAKELVMDDTRLVQVHQIGLNIVEAAKDFEKASYQGEEGVCPH
                     CHNRLFYLEPGTKKAICALCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHGDD
                     IKKMEGHYAEVRKTQEFKERKTSYNFIEPLTKEKQAGVSLG"
     gene            10463..10687
                     /locus_tag="MJE33_002033"
     CDS             10463..10687
                     /locus_tag="MJE33_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647967.1"
                     /translation="MNEKPSFKQIEVILAVIFLVLTYYKVRLTPAFIVIFIPLYLSYF
                     AVSMIVNQIAIKLLEIRQAREATNYNIGNG"
     gene            10680..11192
                     /locus_tag="MJE33_002034"
     CDS             10680..11192
                     /locus_tag="MJE33_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBY9647968.1"
                     /translation="MDRKKKEILTLSMGIALLPPLWAVLAPYIGIKTGAVALICAGLY
                     VTNGNKQKDGLKIMFGFWCGDLWAVLAILIMGYMNFNQNLELFLTLSILGFFAVVIAS
                     LFEKIIFLPSWLCGWAIGLTVMTGENIINLQDICIQIAVAMAVGVWYVGAGVDLFTIY
                     ILSKDRKKGN"
     gene            11224..12078
                     /locus_tag="MJE33_002035"
     CDS             11224..12078
                     /locus_tag="MJE33_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HBY9647969.1"
                     /translation="MAMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGARKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDVENKRVHENVESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGISKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVS
                     ELWFEGEEEWYKAVVEETKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
     gene            12612..13406
                     /locus_tag="MJE33_002036"
     CDS             12612..13406
                     /locus_tag="MJE33_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBY9647970.1"
                     /translation="MYTIGQVSKFLGISRDTLKFYEDKGLVNPKKNDENGYRIYNQVD
                     IYDIATINFYREIDIEIKKIQEIRKSKSINNLELLLEEKEQIILKEIEYKKLLLKKVQ
                     LVKEDCDKIKTYFGKYTIKEMKPLEVKGKIKHVTNYEEYEALKENTNNLKKAVTLTDL
                     RRVINFNEEGIIEEKFIIVREVDDYNKIIEGEVLSHPNCIYTIIEDGRWSTGGENIDN
                     KVEDRLREIAIENECELLGIVYVNILLTTYEEGIERIFLEIYAPIK"
     gene            13510..14334
                     /locus_tag="MJE33_002037"
     CDS             13510..14334
                     /locus_tag="MJE33_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBY9647971.1"
                     /translation="MRTEKSLKRPIASFIILTNIIFLPLFLLVVVTIMLKLPTVISDI
                     ALCISAWSSTFAFMILFKKIYPDKKFLVHVKDRFRNKLKFSTVSIIISIQVLIAVVVI
                     FILASKNHGIMPNFTVSSWGAFIYLFLKNLFAGSLGEELGWRGFVQNHLQKRHSPLKA
                     SIIVGFWWGMWHLPIWFTTGFKGLDLIKYIIFFMIAIISISIIMTTFYNLNKTLIIPI
                     VIHQLFNFLIGIINGDLIELINYYAMLYLIVAIVLVAINPGNALYKRFSKNTTQIL"
     gene            14965..17052
                     /locus_tag="MJE33_002038"
     CDS             14965..17052
                     /locus_tag="MJE33_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBY9647972.1"
                     /translation="MVVDKEIVSILQILLNSTLITTNGLQEESNSSKRQITYRINKIN
                     DMLKVKNVPLICLRADKDIIVRKETKKAIKEILEKNYSKNTYYFSKTERLLYMYLMLF
                     INMEDISINHFINSIKVSRSTVNLDFKDLIPKLEKKNIKVKNNRINGYYIVGNEMEIR
                     RVLIKNIIETLSNEKSSRVFDIFIKEYNLDSFEEAKKIILKLIKKYEITFVEDRLIEF
                     IYIFIFLKARMYSTRIVTQENVDIPNISVMSSMKEYKFAKELLEVYGSEDKIKPHNIM
                     YISAWILGISVGNYEEDTEDKDVISKIVNKMMTKFGYLSGVYYISQEKIFKQLYSHFR
                     PAYYRLLFKLPIYNPICEKVKEEYKLVYRIVSNAMKEFCGLFGEEIQEEELAYLTMHF
                     ATLFSNKKEFDGFRKRIALIVCSNGVGSSAILHAELTNLFPDLHFIPMSESVGLENIS
                     EPVDIVFTTNYNAEGLKTDVPVIKVSPVMTTKEKYKITREVYIQLGDIFLRQPKIDEV
                     MDIIKKYANVTSESILSSELLAYFTQIENFTSKEGEGPMLSEITNETLVKIKVKAKNW
                     EEAIRQSASVLVENNKVTEGYVDAMINSAKASGPYIVITKHVALPHARPEAGSKEIAI
                     GIATLDSPIEFGNNENDPVKYVFCLSAIDNNSHLRAMSELVELLEEDEFFKVLDSAKE
                     AKEIIDFIKTHEL"
     gene            17067..17354
                     /locus_tag="MJE33_002039"
     CDS             17067..17354
                     /locus_tag="MJE33_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY9647973.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIIKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKNLKFDIIGIHNIVDRNEIKTALEKFLASKEG"
     gene            17381..18652
                     /locus_tag="MJE33_002040"
     CDS             17381..18652
                     /locus_tag="MJE33_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HBY9647974.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAVEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGQAFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFISAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNTSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            18710..19309
                     /locus_tag="MJE33_002041"
     CDS             18710..19309
                     /locus_tag="MJE33_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBY9647975.1"
                     /translation="MENTLSEFLDNISNELSEFIASIDETSINKACELILEAEKNHGR
                     VHVTGIGKPGHVSGYISSLLSSTGTSAYILHGTEAVHGSSGQVVEGDVVIAISNSGET
                     QELKATLKTLKVNGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKAPRASILAE
                     TIILQSLSVVLQYAKGLNTQQYLKWHPAGSLGKSIREGI"
     gene            19314..20219
                     /gene="dapA"
                     /locus_tag="MJE33_002042"
     CDS             19314..20219
                     /gene="dapA"
                     /locus_tag="MJE33_002042"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBY9647976.1"
                     /translation="MKYKGIICAMITPFDENQNINSQATCQLIDYLIEKGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVVDLSKRMEAIGASALS
                     IITPYFVTPTQQELILHYKTIANSVNLPIIMYNMPGKTGINIEPESVRELSKIKNIVG
                     IKDSSGKLDNMKAYIEATKNENFSVFSGSDSLILDSLKAGSQGAVAATTNFLTEIGIS
                     IYENFMKGDLEEAQKAQSSIEELRRILKLGTIPSVMKKTIVLNGINVGTARLPVTEPT
                     GEVLEEIKRVVENYRTILNKSSENR"
     gene            20325..20957
                     /locus_tag="MJE33_002043"
     CDS             20325..20957
                     /locus_tag="MJE33_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HBY9647977.1"
                     /translation="MKKMNITKRMSECGALAIVREENLNRACEIAEGCIKGGITVIEM
                     SYTLNNAGEIIQGLNKKYGETLCVGAGTVLDSETARHAILHGAQFIIAPNYNEGVAKI
                     CNRYQIPYAPGCTSLTEAVDALSLGATFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGINIENLHIWLENGVDVCGFGGLLTKGSVEDIAKNAKKIREIIKNVKKI"
     gene            21435..23942
                     /locus_tag="MJE33_002044"
     CDS             21435..23942
                     /locus_tag="MJE33_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBY9647978.1"
                     /translation="MKEDIGLHRMCKKEQATKNEVISEDILKSMSIGVSKHFLDDSFT
                     IIWGNTSFYQLLGYTEEEFLVKFSSLKDYYEQNLYHFESMKNHFLEAYHHGEKSIEYQ
                     ICISANNGDPVWVIMTGILIESQDNEKSMVYILYSKINELKIKQEELSDMEKESTENF
                     KWMMSEYAGNVYISDMDTYELLYLNKHSCNTLQASANELIGRKCYEVIQGRTSPCPFC
                     TNSYLKKDDTYEWEFYNKKLKRTFIIKDRMLNWQGRRVRIELSYDMYSAEYKLAKKDQ
                     ERESILKTIPAGMVRLDARDYLTILWCNDIFLNMIEYTKEQFAEELNNQCGYLFSEDY
                     KRSKKLAQDLKESGDNIVFEAKIYTRSREERIWTVTLCYISGEDSWDGIPSFYSIGID
                     ITKERKQIEKLQHISEKDALTGIYNRAETERQIKKYFEKNLNVMGALFMIDTDNFKQI
                     NDTEGHMIGDIVLTEMASGMKKIMRDSDVVGRIGGDEFTIFMKNISSVKDAEKKAEEL
                     LYMFRHLFQKEKSFLKVTCSIGIAIYPKDGTTFKEIYARADKALYQAKNMGKNNYVIY
                     NQDYFKELEEFDCSSLGTVIDSEKQYAEYPDNLTRYIFRMLYQTDDIDQAINMILEVV
                     GKQFDVSRVYIFENTEDGRYTSNTYEWCNEGISSQMAYLQNSNYQDYEDYEKLFEDEL
                     VFYCRDIHTLSPKLEELFSSQGIHSTLQCAYKEDRVFSGFVGFDECTGLRLWSQEEIS
                     TLSLISQIISMFLQRKKNNKLNQQIQQYQNILDNIDQCICVINKENNFLLYVNKKFKN
                     TYSKFQIGQPYYLDSIDIHKISIIWNKKEAYLCTSMI"
     gene            24284..26446
                     /locus_tag="MJE33_002045"
     CDS             24284..26446
                     /locus_tag="MJE33_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBY9647979.1"
                     /translation="MKKIIYVLFTIELLIVSLLGLNIVKDNEINNILYNDTTSISVMF
                     KDYKRLNKNYSKWIKDIADENNVTISKYVFNNNEKLSIYTTDTSLNNNIKLKSGKFPN
                     TNSNEFISNINTNNSNQVGKFSTMSKDISILIRDFGKISEVGESGILYISTQNEDTIN
                     KILKELNVDRSIFIARLHDRYVNSNLYLNPSIIRDLILIILCFLATIIHYSISVSREA
                     SILRLNGYSKLDIIARVIKNILRIMILSSVTALLIYIIYILKLNIGVRACLYFAMFSI
                     ICILINILISILIVGFNSRSSKYMLSLNGKKSYGFVNIMHFTLKIVFISFLILSINNC
                     ILNYNMLQTQLGNLSEWDKAKNVYNLTLKDTGEQSLGKEEVIKNAKIKGFYKNLVEEK
                     DAFLIDSTNFEKLEDGSYMYEINSKGKNPEVSQYGKNIKINKNYLKVNPIYSNGKEIF
                     NLIDYDDKTINLLVPKKLQVHENEIKKEFRELFYFEKIEVENMYNKELNRELNKTKKS
                     DLNVNIIYVDNNQSYFTYNSLVMDDNRNLIDDPIAIVDIDNIDDSFYLSYISRCVYFN
                     SKKLDALADISNIIEAQGVEAHIQSLHAVYNEYGLEINKLEKYLNSEIFTIIIIAISN
                     LMITYNIVASYYERNKYKLYIKKIFGYSATARSMLLIVSLILTNIIPIGIISTRVDLS
                     NNIILFGLLILVIEVIVSIVLDKIISNSSFNKIIKGEH"
     gene            26451..27068
                     /locus_tag="MJE33_002046"
     CDS             26451..27068
                     /locus_tag="MJE33_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9647980.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAITGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVENNLKIAL
                     KYQKNINHNEKINYALESVGLRKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKSLNKNGKTIVFVTHDLELSEQADRVIKL"
     gene            27257..27601
                     /locus_tag="MJE33_002047"
     CDS             27257..27601
                     /locus_tag="MJE33_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBY9647981.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLGAEEDSNII
                     KDVNGNVLNDGDSVTVIRDLKVKGSSSSIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVVKKA"
BASE COUNT        10795 a         3029 c         4992 g         9036 t
ORIGIN      
        1 gtgatattat caattacata aaaattagtt tatgttatga aatcgttatt gcattataaa
       61 taaatgataa tatcaataat acagggggat aactatggaa aaattcgcaa gttttttaga
      121 gcgcaagatt atgcctgtag cagcgaagat aagtaatcag agacatatga gagctgtaag
      181 agcaggtata atagcaacat taccactaac aatagtgggg tcatttttca caatattatt
      241 aaacatacct attgatgctt atatggattt tatagctcca tttaaagcaa tattagatat
      301 accatttaga tatactgtag gatttttatc tctatatgct acttttggga tagcatcatc
      361 acttgctaaa agctacgatt tagatacaac aggtgtcggt atgcttgcag ttatggcatt
      421 tttagtttct acaatagttc caactcaagt agttgaacca gtaggtggag ttatagaagc
      481 tggtagatgg atggatatgg caaaacttaa ttcgccatca ttatttggag ctatcgtaac
      541 atcgttaatt acagttgaaa tatatcgttt tatgaaggaa aagaatatta caattaagat
      601 gccagcagga gttccagtag aggtttcaaa ttcatttact gcattattac cgacagcagt
      661 aattttaatg ttattctggg taataagaca tattttagga tttgatataa gtgttgcact
      721 aagcgctatg ttatcacctt taaaaggatt cttatcagga aatagtttag gtggtgggtt
      781 actgacagta ttcctaataa ctttattctg ggtacttgga atacatggtc cagctataat
      841 ggggccagta ataagaccta tatgggacat gtctatagca gaaaatataa ctgcattcac
      901 agaaggagta tcagctcatg caatgccaaa catatttaca gaacaatttt tacagtggta
      961 tgtatggata gggggagcag gggctactct tgcattagtt gtaatgttta tgttttctaa
     1021 gtcacaatat ctaaaaagtt taggtagatt aggattttta ccagggttat tcaacataaa
     1081 tgaacctata atatttggag caccaatagt tatgaatcca atactaggga taccatttat
     1141 aattggacct ataataatga caattctatc atatgttctt actattacag gggttatacc
     1201 tatgatggta gcaagattac catttgcaat gccagcacca atagcagcag taatgagtac
     1261 taactggagt atactagcag gtatcttagt attaatcaac tttgtgatat catttgctgt
     1321 ttattatcca ttcttcaaag tttttgaaaa acaacaatta caaagagaac aagaagaaat
     1381 gagtgaaaat ttataaaaat acatagatat agaggttttg tactatataa aatatagaaa
     1441 aagcttataa gttattaagg gtgaaggtag gaggaatgac ctttgccttt aattttatat
     1501 attttaaata atgtaatttg aggaggagaa agatggaaag aagattagga atatcagtat
     1561 atccagagca tagtaacatg gaagatgata aggcatatat aaaaatggct tcaaaatacg
     1621 gatttaaaag aatatttatg tgcatgcttt caattacaag acctaaggaa gaagttaaaa
     1681 aacattttaa agaaattata acctatgcaa gagaattagg atttgaagta atccttgata
     1741 tagctccaaa catatttgat atattacaaa tatcttatga tgatttaaca ttttttagtg
     1801 agttaaatgc tagtggaata aggcttgact atggatatga tggaagtaaa gaagctatga
     1861 tttctttcaa tccatatgat ttaaaagtgg aactaaatat gagtaatgat gtagcatact
     1921 tggataatat aattacttac aagccaaatg taaataatat atatggttgt cacaattttt
     1981 atcctcaaag gggtacaggc ttgccatatg atttttttgt aagatgttca aatagattta
     2041 aaaagtatgg aataagaact gctgcttttg ttaattcaaa atacgcaaca atagggcctt
     2101 ggaatataaa tgatgggctg tgtacgcttg aaatgcatag aaacttagat attgctacag
     2161 caactaagca cttattcgca acaggattga ttgatgatgt aattgtaggg aatgcatatg
     2221 caagtgaaga tgaattaaaa agaatgtctg aagtaaatga ataccaagtt gtatttaatg
     2281 tagtagctga agaaaaaact tctgaagtag aaaagataat aatggtaaat gagcaacact
     2341 ttagaagagg agatataact aatcaggtag caagatctac agaagtaaga aaaaaatatg
     2401 ctaaagaaaa tttccctaaa catgataata agaacctctt taaaagagga gatgtagtga
     2461 ttggtaataa tgactttgga aagtataaag gggaattaca gttgatactt caagattgta
     2521 cagatgaaag aaaaaataag gtgggtagaa taaaggaaga cgaaataata cttttagact
     2581 ttattgaacc ttggagtaaa tttaagatta ttttagaggg agagtaaagt gaagatagac
     2641 atattaataa aatgtggaaa aacagtagat aaaagtataa ttgatatagc tatattagac
     2701 gataaaataa tagaagtaaa aaatcagata gatgattcta agtatcaggc aaaacaaatt
     2761 ataaacttag atggagagaa atttattagt gctggttgga tagatataca tacgcattgc
     2821 tacgaaagac tagaccttta taaggattat ccagatgagg ttggaataaa aagtggagtt
     2881 actactgtag tagatgcagg aacaacagga gctttagata taggtaaatt ttatgaagat
     2941 actaaaaaat ataaaacaaa tgtatatgct ttaataaata ttgctaagca gggaataaca
     3001 tctcaagatg aactttcaag tatgatgaat attgatgaat atgaacttaa aagagcagtt
     3061 aaaaaataca aagattttgt tgttggaatc aaagctagaa tgagtaaaag tgttgtaatt
     3121 tcaaatgatg ttgaaccttt aaaagtagct aaaagaataa aaaatgaact taatctacct
     3181 atgatggttc actttgggag ttctcctcca acgatagagg atatatttga ctatatggaa
     3241 aaaggagata tattaaccca tatatataat ggaaagccaa atggaatact tagaggcaat
     3301 gaggttaaaa aagaaatcat agaagctaga gaaagaggta taatccttga tgtaggtcat
     3361 ggaaccgaaa gttttagtat ggatattgca atgaaatcta aggatgcagg aatttttcca
     3421 gacactataa gtacagatat atatattaaa aatagaataa atggtccagt gtataatcta
     3481 tctacaacaa tggaaaagtt tatatatatg ggctattcac ttgaagatat aataaataaa
     3541 gttactaaaa atgcagcaga tgcaataagt ttaaaaaata aaggtttaat aaaagaagga
     3601 tatgatgctg atttaactat atttgatgtg gttaatgaag aaaaagaact tcaagattct
     3661 ttgaataagt cagtaatcac aagtacgtct ataaagccaa gagcagtagt agtcaatgga
     3721 gaatatttaa atataggtaa gtctatagaa gagaatgagt agaagggaga aactattgta
     3781 tgagtagtat atttgaaaaa tactctgtaa gacgtgttat aaatgcttca ggcaaaatga
     3841 ctatactagg agtatcaaca gtagataagg aagttattga agcaataggt gaagggtgta
     3901 aaaatttctt tataatagaa gaacttataa ataaaacagg tgaatatata gcaaatttat
     3961 tagggtgtga aagtgcacta atagtttcta gtgcatctgc tgggattgca cagagtgtgg
     4021 gagcaatgat agcaaaagat gatatgagtc tagtttacaa tgtaaataat cctaagaaac
     4081 aagttaaaag ggaaataata attccaaagg gacataatgt tgattatgga gttccagtag
     4141 aagtaatgat aagacttgga ggaggagaag ttgttgaggc cggttatgct aatatgtgta
     4201 acaaggaaca tataataaga gagataaatg aaaatacagc agcaattatg tatattaaat
     4261 ctcatcattg tgttcaaaag agtatgctaa gtgtattaga tgtagtacaa gttgctaaag
     4321 aatataattt acctttgata gtagatgcag cagcagaaga agatttaaat gtatattatg
     4381 aattgggagc agacctagtg atatacagtg gtgctaaggc tatagagggt cctagctcag
     4441 ggttagtaat aggtaagaaa aaatacatag ataatgtaaa gcttcaatca aagggtatag
     4501 gaagagctat gaaaatagga aaagaaaata ttgtaggtct aacatgtgca attgagagat
     4561 atataagtaa tgataaagta actttaaaag aaatggagga caagctaaat ccatttatag
     4621 acaagataaa tactataaag ggagtgagtg ctagtataac tagggattca gcaggtagag
     4681 aaatacttcg tggagaaatt gactttaatg aagatataat aaataagtca acacaacaaa
     4741 ttataaatga acttagaagt ggagagatag ctatatacac tagagactat aaggctaatg
     4801 aaggcaagat tgagattgat ataagaagtg taactggaag tgatttagat actatttata
     4861 ataggataaa aaatatagta gaaagttaga gtgtagagta agtgacatta taatgattta
     4921 ataaattaaa ttttaccaat ggaggggata aatatgaatt gtataccaca atattataaa
     4981 aatagagttt gtttaaatgt tcttgcagga agcataaaaa atgctaaaga agtatatgat
     5041 gcttgtgacg ggcatgtatt gataggagta ctatcaaaga attacagtac tgtagaagca
     5101 gctattgaag atatgaaaaa atactcaaag gaaatagata atgcaatatc tgtggggctt
     5161 ggagctggag acccaaatca gtcaaatatg gtatctcaga tttctaaaga acttcagcca
     5221 caacatataa atcaagtgtt tacaggtgtg gctacgtcta gagcattact tggtcaaaat
     5281 gatagtataa taaatggatt agtttctcca actggtaaac caggatttgt aaaaatatcc
     5341 acaggtccat taagttcaaa agaaaaagat ggtatagttc ctatagaaac agcaatagct
     5401 atgatgaaag atatgggagg aagctcaatt aagttttttc caatgggagg acttaaaaca
     5461 aaggatgaat atatagaagt tgcaaaagct tgtgcaaagc ataattttta tttagaacca
     5521 acaggaggta tagacttaga taacttcaaa gagatagttc aaattgcact tgatgctggt
     5581 gtagaaaaag ttatacctca tgtttataca tcaataattg ataaagaaac aggtgaaacg
     5641 aaagtggaag atataggagt actttataaa ataatgaaag aattattggg atagatttat
     5701 gaatatatta gcttttggtg aaataatgat gagactttca gtacctgact acaagttctt
     5761 aacacaaacc aatgaactaa attatataat tacaggcacg ggattaaata tattatctgg
     5821 acttaaaaac tttggataca atacctatat gttgacaaag ttgcctaata ataatgtagg
     5881 taaagcatta agtgcaaata taagaaagct tggagtaaaa gatgacttta tcacatatgg
     5941 agggaatcat ataggagtgt attttcttga aaatgggtat ggagaaagac cttctgaagt
     6001 gacttatttg aataggttaa atagttcatt ttgtgagagt aaattacaag attatgatat
     6061 tgataaatgt ttagaaggaa tggatatagt ccatatttgt ggtattgcta tgcaacttac
     6121 agattcagtt aaagagattg ctttagaact agctagaaaa tcttatgaaa aggggataaa
     6181 agtattcttt gattttaatt ttagacaatc attaaatcca gaaaggactt atgatgattt
     6241 agtaaatgat tataagaaaa tactaccata ttgtcatggg ctgtttgcta gttatagaga
     6301 tataaataag atacttaata tagatagtct aaatataaaa tctagttcag aattgattaa
     6361 aaaagagtat gatataggta ttgtagctgg aactatgaga agatttgatg atatgaataa
     6421 tagatttata aagggatata tatcaaatga tgatggttat tttgaaagta gggagtataa
     6481 gttagaaata tatgacaggg ttggtgccgg agatggatat gtagcaggaa ttatatattc
     6541 atatttggaa aatatgaaga gtgaagatat agttgagttt ggggttgcta gttcggtgtt
     6601 agctcataca acttatgggg ataattcttt agtaagcaca ggtcatgtac ttagactgat
     6661 gaacaatgaa aaaatagata tattgagata atacatttgt atataataaa atttttatct
     6721 tttttagaca catttttgac ttgcttatat ttgttaaaca gagttatttt taatgattat
     6781 aaatattaaa ttaaaagtta aataataaaa tagtagaaaa tagcattaac tatagaaaaa
     6841 atggttaatg ctatttttat cgttaatatt gattaatttt atattataga aatgaacttt
     6901 tctaatgctg gatttgtatt ttttttattc catacaacta caacttcaat tctattttct
     6961 acttctttta taggtactat tgcaatatca cgttctatca ttgtatctgt aacacaaaat
     7021 gaaggtaaaa tggatattcc catgttagaa gataccaatg attcaatagt attaagatta
     7081 tttgaacgtt gtaccacatt aggtacaaac cctgcttcac gatatctatt ttgtatcata
     7141 gatttttgtc catactcatc ccctgtttcc tttacaaaaa taaatttatc atgttgtaat
     7201 tcaattaaat cgatagaatc ctcattgtaa agtggatgac caggaggtaa aagagctacc
     7261 aaagaatatc tttctacagt tttatattca aaatctttta atggttgatt ttctggatta
     7321 ataacaaaag cgaggtccat agaattattt tttactaagt ttaaaagttc ggttatttca
     7381 ttagtacaaa tatccataga tatattaggg tatgtattag aaaattcttt taaatattgt
     7441 tgaagtttgt ttttttcata tcctattaag gttccaatgc tcaaagttcc aaaaaaacca
     7501 tgtgcaaatt gttgtgtttt tgcaatagca tcattgatat tttttattat agtacgtgcc
     7561 tcagataaaa aaacttttcc agctggtgtt agttcaacat gacgtttact tcttgtaaat
     7621 agagttacat ttatttgttc ttctaaagct tttatttgct gtgtaacagc agtttgagaa
     7681 atataaaatt tttctgctgt tttggtaaaa cttagctttt ctgctacaga tataaaatat
     7741 tctaattgtt tagtattcaa ataatcaccc ctatttaaac ttgattgttt gtaaacctta
     7801 tttatctata attaaagctt attatagtat aagaaaattt acttttaaag aatattagaa
     7861 aatatttgta taaaaatata tgttttaagt atatctttac attagatttt ataaatttat
     7921 taataagaat aacttatata ttccaatgaa aacgatattg gatttaagta agtttgtctc
     7981 ataaaatgaa agtatatcaa ttgataatac tttaaaataa gaagggagaa aaaatggaag
     8041 ataaatttta tgcaaaaggc aacggaaata acggatatat taaaaatctt gaagtttgtt
     8101 cctttaataa cttagatgga acttgtggaa tgtttcaaat ggctctgtac aaaagagatg
     8161 aaaaatacta tttatatgga tgctgttttg gaggaaataa aaaaaatgga gtaatgatta
     8221 gcgatattac agacccttat aatccacaat ttataaaaca ttttcaaatg ttagacccta
     8281 aagagtatcc tacaacaaca actcccaaaa ttcaaatagc agatgattta atgatagtag
     8341 caatgagttg tggaagtgga ccaggagcac ttgttgacca agctaaatta gcaaatatta
     8401 agtgtgaagc aggaattaga atatacagtt taaaagaaga ccctttaaat cctaagtttt
     8461 taggatattg ggattgtggc ttaaagcatg taatgggtgt tcatagattc atgtacaatg
     8521 gtggaagata tgtacattta tcaagtgatt gtgttggctt tgaaggtctg atttataggg
     8581 tcatagatat aataaatcct actaatccag tggaaatagg taaatggtgg agaccagacc
     8641 aatatgcaga tggatatcca aatagaactt ttgatgcagg agcacctcat tgcccagaat
     8701 ttatggataa aggatggctt catggacctc catttgtaag agacggaaaa gcatattgtg
     8761 gttatggagg agctggttta gttgtattag atgttgaaga tttaacaaga ccaagatgct
     8821 taggtgaatt gccatttacg cctgcatttt ctagtagact tgcaggtgca agaactcata
     8881 cagcattacc attgccagga agagatttag tcgttgttca aaatgaggga gaaagattcc
     8941 agttctttaa accagataac attacagatg ttcaagctat gaataatata catatggttg
     9001 atgttagtga cccaacaaaa ccaacattaa ttgctcaatt tccatatcct gaagttccaa
     9061 aagatttccc ttatcctaac tttaatgttg caggattagg aaaaccaggg ccatttggtc
     9121 cacataatct tcatgaacca atggataata agccatggtt agagcaaaga ggagatagag
     9181 tatattgctg ttatttccat gcagggctaa gggtttatga tgtatcagac ccatattata
     9241 tcaaagagct agcatatttt ataccaccaa atccaaataa aacaccagaa gaatcttatt
     9301 tcccaggatt cccaggacca cgcttggcag taacagaaga tcttatcgtt gatgatagag
     9361 gctacatcat catagatgct ttagatgatg gattctatat attaaaaatg aaagaagatt
     9421 aaataaatac taaagaggga gagttgaaaa tgaaaatact aggtatttct tttggaacaa
     9481 aaaatggaaa taatgacaca atgtgtaaag ttgcacttaa aggtgccaaa gaagcagggg
     9541 ctgaagtaga atttattcaa atgtcatcta ttaacattaa acattgtact ggatgttgtg
     9601 cgtgtgttaa aactttatta tctgggaaag gtagtatgtg tgtattaaaa gatgattttg
     9661 aatggttgct tgacaaaatg aaagatgcag atggtatagt agtttcagac cctatttttg
     9721 aagaaggggc atcaggatta ttccatacta taatggaccg ttttggaccc agagcagata
     9781 gaggaaataa cattatagga acaaaagttg cagaagttat aggaggaaaa attccagatt
     9841 caagaatgct taatgataaa gtaatatcat ttatgggaat aggaggttct gactggggta
     9901 caagagttca atgtgaacat gctatgctag ctctcatacc aatgtggaaa gttatagata
     9961 atgcttggtt cccatgggca aaggaattag taatggatga tacaaggctt gttcaagttc
    10021 atcaaattgg tttaaatata gttgaagcag caaaagattt tgaaaaagct tcatatcaag
    10081 gagaagaagg tgtatgtcca cattgccaca atagattatt ctaccttgag ccaggtacta
    10141 aaaaggcaat ttgtgctcta tgtggtatag ttggagagtt agatactata agtggaaaaa
    10201 ctatatttag ttttccagaa gagcaattag gtcatgctca tgatacttta ccaggtaagt
    10261 ttatacatgg tgatgatatt aagaaaatgg aaggtcatta tgcagaagtt cgtaaaacac
    10321 aagaatttaa agaaagaaaa acatcttata attttataga gccattaact aaagaaaagc
    10381 aagctggagt ttcattagga taataattaa aatactaaga ctctttttta agggatgtta
    10441 gatgttatag gaggcttatt gtatgaatga aaagccaagt tttaaacaaa ttgaagttat
    10501 tttagcagtt atttttttgg tacttacata ttacaaggtt agattaactc cagcatttat
    10561 tgttatattt ataccactat atttaagtta ttttgcagta tctatgattg taaatcaaat
    10621 agcaattaag ctattggaga taaggcaagc tagggaagct actaattaca atataggaaa
    10681 tggatagaaa gaaaaaagaa atattaacac tgtctatggg gattgcttta ctacctcctt
    10741 tatgggcagt attagcacct tatataggaa taaaaactgg agctgtagca ctaatctgtg
    10801 caggattata tgtaactaat ggtaataaac aaaaagatgg attaaaaata atgtttggtt
    10861 tttggtgtgg agatttatgg gcagtgttag ccattttaat tatgggatat atgaatttca
    10921 atcaaaacct agaattattt ttaacactta gcatattagg tttttttgca gttgtaattg
    10981 catccttatt tgaaaaaata attttcctac catcatggct ttgtggttgg gctataggtc
    11041 taactgttat gacaggtgaa aatattatta atttacaaga tatttgtatt caaattgctg
    11101 tagctatggc agtaggagta tggtacgtgg gtgcaggtgt agatttgttt acaatatata
    11161 ttttgagtaa agataggaaa aagggaaatt gaaagcatgt gaatatactt tgattatagg
    11221 agaatggcta tgggttttga atttaaaata atgagaagtt taatatatgt aggacttgcc
    11281 aaggaagaat atagacctaa gctaatggac tggttatatc gtcaccatat tccagatagt
    11341 attagcactt ttggaccata ttgtactaaa tatgcctttt atcaagcata tcctacacca
    11401 aatgaaggtg agcgttttgg tgcacgtaag atgcaactaa cagaacatta ttggcttgta
    11461 gatgaacata tgcctgagat ggcaaataga attatgacag aatatatgcc tatggatgtt
    11521 ctacgttggc aagggtgtat accagatgta gaaaataaaa gggttcatga aaatgtagaa
    11581 agtggagatg caggacgtgc agtaggtgga gataatggat gtccaccatt tatatttgcc
    11641 tttgttccaa taaactggga agaagacttt agaggaaaag gacgtactgt acaagatgga
    11701 ccaaactatc gttggcaatt tatgattaag tatccagatg gtatctctaa agaagaagga
    11761 gaaaaatggt tctatgatga ggtagtgcca tactttacaa actgttgcta tgttaatcgt
    11821 tttgtcagta gtaaaataat gattaattat ggagcaactg cttttgaccg tgtatcagaa
    11881 ctatggtttg aaggggaaga agaatggtat aaagctgtgg ttgaagagac aaagtcgttt
    11941 attaaaaaac cagaatgggc acaagaagag gagttcccat atttaaaacc acaattcaat
    12001 atcgcatcag tattcttagg tgatatagca actatggatg catactcaca gtatcgtgga
    12061 tatataccaa tgagataaaa attatagttt gtgataagta gaggaggcat ttactcttac
    12121 tggtagaaga agacttattg tatccttaaa agtaggtgaa tatatagttt tatatttaaa
    12181 tttatataaa actaagacta ataaatatga aacaatagaa ttagaataat ttgataatat
    12241 ttgttaaaag agcatttaga atgtgtttaa agattaagat attcctttta tggtagcaga
    12301 tagttgaaat aataaaactg ttatcatgaa aggaatattt ttatgtatag gaaattttta
    12361 aaaataaaag ttaaatatgt ttaagaacat cttaaagtga aagaatcatt gttagaaata
    12421 tgtagacaat atagtcttta taacgtatca tattaacaag tttacacttg gattatgaaa
    12481 taaaaagagt aattccaaag gttgtaaata ctatgctgac tttgatactt caaagtttag
    12541 gtgattttgt aattgttaat agaaatacgt ttaatgagca taaagaattt atctaataat
    12601 aggagaatat aatgtataca attggacaag tatctaaatt tttaggaata tctagagata
    12661 cactaaaatt ttatgaggac aaaggattag taaatcctaa gaaaaatgat gaaaatggat
    12721 atagaatata taatcaggtt gatatctatg atattgcaac aataaacttt tatagagaaa
    12781 ttgatataga aattaaaaag attcaagaaa taagaaaaag taagagtata aacaatttag
    12841 aactcttact agaagagaag gaacaaatca tactaaaaga gatagaatat aaaaagcttc
    12901 tcttaaaaaa ggttcagtta gtcaaagaag attgtgacaa aataaaaaca tattttggaa
    12961 aatacacaat aaaagaaatg aagcctttgg aagtaaaagg taaaataaaa catgttacta
    13021 actatgagga atatgaagcc ctaaaagaga atacaaacaa cttaaaaaaa gcagttacat
    13081 taactgattt aagaagagta ataaatttta atgaagaagg tataatagaa gaaaaattta
    13141 taattgtaag ggaagtagat gactacaaca aaatcataga aggtgaagtt ctatctcatc
    13201 ccaattgtat ttatactatt attgaagatg gcagatggtc aactggagga gaaaatattg
    13261 ataataaagt ggaagacaga ttaagagaaa tagcaataga aaatgaatgt gaattacttg
    13321 gaatagtcta tgttaatata ttgcttacta cttatgaaga agggattgag cgtatatttt
    13381 tagagattta tgcacctata aaataatata aaagcattga cctgggactt actcataggt
    13441 ttaaactcat gatataagca gttataactt atatagcaat taaatattaa tttgaaatga
    13501 ggattactta tgagaacaga gaaatcatta aaaaggccta tagccagttt tattattcta
    13561 acaaatataa tttttttacc acttttttta ttggtagtag tgactataat gttaaaactt
    13621 ccaacagtga tatctgacat tgcgttgtgt atatcagcgt ggtcatccac ttttgcattt
    13681 atgatattat ttaaaaaaat ttatccagat aagaaatttt tagtacacgt aaaagataga
    13741 tttagaaata aacttaaatt ttcaacagta tctataataa ttagtataca agtcctaata
    13801 gctgtagtag ttatatttat attagcatct aaaaatcatg gaataatgcc taactttaca
    13861 gtttcatcat ggggagcatt tatatattta tttttaaaaa atttatttgc aggatcatta
    13921 ggtgaggaat taggttggag aggatttgtt caaaatcatc ttcagaaaag acattctcct
    13981 ttaaaagcat ccataatagt aggtttttgg tgggggatgt ggcatttacc aatatggttt
    14041 acaacaggat tcaaaggact tgatttaata aaatatatta tattttttat gattgcaatt
    14101 atatcaatca gtattataat gacaactttt tataatctaa ataaaacttt gataatacct
    14161 attgtaatac atcaactatt taatttttta atagggataa ttaatggaga tttaatagag
    14221 cttataaatt actatgcaat gttatattta atagttgcaa ttgtattggt agctataaat
    14281 ccaggaaatg ccttatataa aagattttct aaaaacacaa cacaaatact ttaataatat
    14341 aagtgagaat catatcacaa atgagagaaa taattctcct tattaattaa ttttaattaa
    14401 taaatattac aatagattta tctggaatgg aaggtatgca gagtgtattt cattagatga
    14461 aacacttaaa agaatgaaat agataagtat caacttatta tatgagagaa actttaactt
    14521 tcttggttta aaatattaaa attataagga acatttatta ttaaatactt tatttatatg
    14581 aattaattga agaaaaagga tattaaacta caagttaaaa aataaattta atatattaga
    14641 attataaata aggcacatta attttatttt aaattaatgt gccttttatt tatggtaatt
    14701 tatgcataat tagtatcgtt ttgattcttt aaataaattt ttcttggttt cttcacataa
    14761 ggaacttttt ttaatttata aaatctacag ttatattata gtatctattt tagagaagtt
    14821 taatttttgt atcaatctaa aacaaaagtt atacttaaat gaaattgaaa acgataaaat
    14881 tctcaaataa aataagtatt gtggaggtga tgaaagaatc caatgataat atgaagacta
    14941 atcttaataa gaaagtaggg gttaatggta gtggacaaag aaattgtatc aattctccag
    15001 attttattaa atagcacatt gattacaact aatggattac aagaggagtc taattcatca
    15061 aagagacaaa ttacttatag aataaataaa atcaatgaca tgcttaaagt aaaaaatgta
    15121 ccacttatat gtttaagagc tgataaagat attatcgtta gaaaagaaac aaaaaaagca
    15181 attaaagaaa ttttagaaaa aaactattct aagaatactt attattttag taagacagaa
    15241 agacttttat atatgtattt aatgcttttt attaacatgg aggatatttc aattaatcat
    15301 tttataaatt ctataaaagt aagtaggtct actgtaaatc ttgatttcaa ggatttgata
    15361 ccaaagcttg aaaagaagaa cattaaagtt aagaacaatc gtataaatgg atattacata
    15421 gttggcaatg agatggaaat taggagagtg cttattaaaa atattataga aactttgtct
    15481 aatgagaaaa gttcaagggt atttgatatt tttattaaag aatataattt agatagtttt
    15541 gaagaggcta aaaaaattat attaaagcta ataaaaaaat atgaaattac ttttgttgaa
    15601 gatagattaa tagaatttat atatatattt atctttctaa aggcaaggat gtattcaact
    15661 agaatagtta ctcaggaaaa tgtagatatt cctaatattt cagttatgag ttcaatgaag
    15721 gagtataaat ttgctaagga attattagaa gtatatggga gtgaagataa aataaagcct
    15781 cacaatataa tgtatattag tgcttggatt ttaggtatat cagtaggaaa ttatgaagaa
    15841 gatacagaag ataaggatgt aatttcaaaa attgtaaata aaatgatgac aaagtttgga
    15901 tatttaagtg gagtttacta tataagccaa gaaaaaatat ttaaacaatt atattctcat
    15961 tttagacctg cctactatag acttttattt aaattaccta tctataatcc gatttgtgaa
    16021 aaagtaaaag aagaatataa attagtttat agaattgtaa gtaatgcaat gaaagagttt
    16081 tgcggattat ttggagaaga aattcaggaa gaagagttag catatcttac aatgcacttt
    16141 gcaacacttt tttcaaataa aaaagaattt gatggtttta gaaaaagaat agcattaata
    16201 gtttgttcaa atggcgttgg tagttcagct attttacatg cagaacttac aaacttattt
    16261 cctgacttac actttatacc aatgtcggaa tcagttggat tagaaaatat aagtgaacct
    16321 gtagatattg tatttactac taattataac gcagagggtt taaaaacaga tgtaccagta
    16381 attaaagtaa gccctgttat gaccacaaaa gaaaaatata agattacacg tgaagtgtat
    16441 attcaattag gtgatatttt tttaagacaa cctaaaatag atgaggttat ggatataata
    16501 aaaaaatatg caaatgtaac ctctgaaagt attctttcaa gtgagctatt agcttatttt
    16561 acacagatag agaattttac atctaaggaa ggagaggggc ctatgctaag tgagattact
    16621 aatgaaacac tagtaaaaat aaaggtaaaa gctaagaatt gggaggaagc tattagacag
    16681 tcagcttctg tattagttga aaataacaaa gtaactgaag ggtatgtaga tgctatgatt
    16741 aactcagcaa aagcatctgg accatacata gtaattacaa aacatgttgc acttccacat
    16801 gctagaccag aagctgggtc aaaagagatt gcaataggca ttgctacttt agatagtcct
    16861 attgagtttg ggaataacga aaacgaccct gtaaaatatg tattttgtct aagtgcaata
    16921 gacaataata gccatttacg tgctatgtct gagctagttg aactattgga agaagatgag
    16981 ttttttaagg ttttagatag tgctaaagaa gctaaagaaa ttattgattt tataaaaaca
    17041 cacgaattat aaaggaggat ttaattatgt ataaaatttt aatcgcatgt agagcaggag
    17101 ttggctcaag tttaatgtta aaaataaaga ctcaacaaat tattaaggaa aataactttc
    17161 caattgaagt agaacatggt tcattagatt cactaaatgg attcaatgga gatgctgtag
    17221 aaacattaat tgatgttgct gaagaattaa aaaataaaaa tttgaagttt gatataattg
    17281 ggattcacaa tattgttgac cgtaatgaaa taaagacagc actagaaaaa ttccttgctt
    17341 caaaagaggg ataaatttaa gatgataagg aggaatattt atggagttta ttattagttt
    17401 gctgagtaca cctgctgttt tgcttggcgt agttgcatgt ataggattga ttcttcaaaa
    17461 gaaatcagct gttgaagtat ttacaggctc ttcaaaaaca cttatcggtt ttttgatatt
    17521 tggtattggt gcctctgcta tgacaggagc acttcaaaat ttcaataaac tgttccaaca
    17581 tggatttgga attactggtg ttatagcttc accagaagca gctacagcac ttgcacaagg
    17641 tacatatggt tttgcagttt catgtacatt aattcttgga ttcatattaa atttagtatt
    17701 tgcacgtatt acaaagttta agaatatttt ctttacaaca ggtcatagtt tgttcttttc
    17761 ttgtgtatta gttttaatta tgaaagcaca tggatttgat aatacaatca ctattttaat
    17821 aggaggaact atcttagggt ttatgtctgc tgcactacct caattctgtc aaccatttat
    17881 gcgtgaatta acaggcggcg acgaacaagc tattggtcac tttaacatga ttggttatgg
    17941 tttatcaggt tatattgggc gtttattttc aaaacataaa gatgatacaa cagagactat
    18001 agaatttcct aagtggttat caatattcag agacttctta atgggtctat ccatagttat
    18061 gttaatctta ttttatattg caacattaaa agctggtcaa gcatttactc aagaaattgc
    18121 tggttcaaca cattggttgg tttttccgct tatagaggca tttacatttg tggcaggtat
    18181 gtcaatattg atgtcaggtg ttcgtatgtt cttagctgaa ataactgcag catttgtaat
    18241 catttcagaa aagtatattc caggttcacg tcctgcgcta gatgttccaa ccgtatttcc
    18301 atatgcacca aatgcagtta ttattgggtt tatatcagca tatgctgctg gtctattagc
    18361 aattgctatt atggcatcat ttcctagtat atttcctgta gtaattattc cagctgctca
    18421 catatgtttt ttctcaggag gtacagctgc aatatttggc aatacttcag gaggatggag
    18481 aggggctatt gctgggtcat ttgttgtggg gttattactt gcatttttgc cagttatact
    18541 atatccagtt tatgggactc ttggtataga aggagcaact ttccctaata ttgattacaa
    18601 cgtagttggt tcgatattac ataatatttt acaatttata aaacctattt aaactaaatt
    18661 atataaaagt atagtcatag ttactatgat ttcaaggagg atacatatta tggaaaatac
    18721 attatctgaa tttttagata atatatctaa tgaattgtca gaatttattg cctcaataga
    18781 tgagacatct ataaataaag catgcgaatt aattcttgaa gctgaaaaaa atcacggacg
    18841 tgtacatgtg actggtattg gcaaaccagg acatgtatcg ggatatattt catcattgct
    18901 ttcatctact ggaacgtcag catatatatt gcatggtaca gaagcagtac atgggtcaag
    18961 tggtcaagta gttgaaggag atgttgttat tgcaatatca aatagtggtg aaactcaaga
    19021 gttaaaagca actcttaaga ctctaaaagt aaatggagca aagataattg gtgtaagtgg
    19081 taatgagagt tcatttttaa aaaatatttc tgatatattc ctgtttgcag gtgttaaaca
    19141 ggaaggagat tgtctcaata aagctcctcg tgcttcaatt ttagctgaaa caataatatt
    19201 acaatcatta agtgttgtat tacaatatgc taaaggtctt aatacacagc agtatttgaa
    19261 gtggcatcca gcaggaagtc ttggaaagtc tattagagag ggaatataat tttatgaaat
    19321 ataaagggat tatatgtgca atgataacac catttgatga aaatcaaaat attaattcac
    19381 aagcaacatg tcaattaatt gattatttga ttgaaaaggg aatttatgga ttatttattt
    19441 tgggcactaa tggtgaatgt catgtattaa cagatgatga aaaagttgaa tttgcaaaga
    19501 ttgttattaa tcatacaaat aatcgtgtac ctgtttttgt tggaacaggg ggtaattcaa
    19561 ctcgtgaagt tgtagattta tctaagagaa tggaagcgat tggagcaagt gcattatcta
    19621 ttattacacc atactttgtt acaccaacac aacaggaatt aatcttacat tacaaaacta
    19681 ttgctaattc agttaattta cctattataa tgtataatat gccaggtaag actggaatta
    19741 atattgaacc agagtcagta cgtgaattat caaagataaa gaatattgtt ggaattaagg
    19801 atagtagtgg taaacttgac aacatgaaag cttatattga agcaacgaag aatgagaatt
    19861 ttagtgtgtt ttcaggctca gattcactta ttctggatag tctaaaagct ggtagtcaag
    19921 gagctgttgc agcaacaaca aacttcctca cagagattgg tatctctatc tatgaaaact
    19981 ttatgaaagg ggatttggaa gaagctcaaa aagctcaaag tagtatagaa gaacttcgac
    20041 gtatactgaa acttggaaca attccatctg ttatgaagaa aactattgtc ttgaatggaa
    20101 ttaatgttgg aactgcacga cttcctgtaa ctgaaccaac tggggaagta cttgaagaaa
    20161 ttaaacgagt agttgaaaat tataggacaa tacttaataa aagtagtgag aatagataat
    20221 ataaataggg aaattacact ggtatataat taatatatat attataaaac atgactaaaa
    20281 ttaatgaata agtaaagtgg tttgatgagt atagaggtga taatatgaaa aagatgaata
    20341 taaccaaaag aatgtcagaa tgtggggctc ttgcaattgt aagagaagaa aatcttaacc
    20401 gtgcatgtga aattgctgaa ggatgtataa aaggtggtat aacagttata gaaatgagtt
    20461 ataccctaaa taatgctgga gaaataattc aagggcttaa taaaaagtat ggtgaaacac
    20521 tttgtgtggg agcaggaaca gttttggata gtgaaacagc tcgtcatgct atattacatg
    20581 gagctcaatt tattattgca ccaaattaca atgaaggtgt agcgaagatt tgcaatagat
    20641 atcagattcc ttatgctcca ggatgtacat cattgactga ggcagtagat gcactaagtt
    20701 taggagcaac ttttataaaa gctttcccaa tatcagattt ctatggacct aaactagtaa
    20761 aggtatttaa gacaccaata cctgatatgc cagttttagc aagtggaggg attaatattg
    20821 aaaacctaca tatatggtta gaaaatggag ttgatgtatg tggatttggt ggtttattaa
    20881 ctaaaggttc tgttgaagat attgctaaaa atgctaaaaa gattcgtgaa atcattaaaa
    20941 atgtaaaaaa aatttaacat agggctgtat catagtatct tataattact atgatacagc
    21001 ctttttaata ttattcaaat ttaaaacagt ttacatttct attatattac atataatttc
    21061 agtaatttta tattaatttt tctatctgga aaaaaatgat ttaaaactta agttatgata
    21121 taatttcatt aaaagaaggc gatttttagt aaatatgaga aaataatgtg tttttagggg
    21181 aaaagttaaa tacagattta agtgaacaag aatcgataat taagatatta aatggtgaat
    21241 agggcatcaa aaaaattact aaattaaaag ttttagaggg agaatcttag caagtgtgtg
    21301 tgaaaattca atttcaaaca aaaaataatt gtaaatgaaa tcaatttata cacgttgaag
    21361 attcactttt atcattatat taaaaagcag agtgaattaa aagtgagtct aaagatagga
    21421 ggataattaa aacaatgaaa gaagacattg ggttacatcg aatgtgtaaa aaagaacaag
    21481 caacaaagaa tgaagttata tcagaggata ttctaaaatc tatgagtatt ggggtcagta
    21541 aacatttttt agatgattca tttacaataa tatggggaaa tacatcattc tatcaattat
    21601 taggatatac agaagaagaa tttttagtta aattttctag tttaaaagat tattatgaac
    21661 agaatcttta tcattttgaa tctatgaaaa atcatttttt agaggcttat catcatggcg
    21721 aaaagagtat agaatatcaa atttgcattt cagcaaataa tggagaccct gtttgggtaa
    21781 taatgactgg tatattaatt gaatcacaag ataatgagaa atcaatggtc tatatattgt
    21841 attcaaaaat taatgagtta aaaataaagc aagaagaact atctgatatg gaaaaagaga
    21901 gtacagagaa ttttaaatgg atgatgtcag agtatgctgg gaatgtttat attagcgata
    21961 tggatacata tgaattacta tacttaaata agcactcatg caatacttta caggcatcag
    22021 caaatgaact tattggaaga aaatgttatg aggtaattca aggtagaact tcgccatgtc
    22081 cgttttgtac aaattcgtat ttaaaaaaag atgacacata tgaatgggaa ttttataata
    22141 aaaaactgaa acgaacattt ataattaaag accgtatgtt gaattggcaa gggcggagag
    22201 ttcgtattga attatcttat gatatgtata gtgctgaata taaattagca aaaaaagatc
    22261 aggaaagaga atcaattttg aaaactattc cagcaggaat ggttagatta gatgcaagag
    22321 attatcttac tatactttgg tgtaatgata tatttttgaa tatgattgaa tatacaaagg
    22381 aacagtttgc agaggaactt aataatcaat gtggatattt attttctgaa gattataaac
    22441 gtagtaagaa actggcacaa gatttgaagg agtctgggga taatattgta tttgaagcaa
    22501 aaatatatac acgttcaagg gaagaacgta tatggactgt tacattgtgt tatatcagtg
    22561 gggaagacag ttgggatgga ataccatctt tttatagtat aggtattgac ataacaaagg
    22621 aaagaaaaca gattgagaaa ttacagcata tatcagagaa agatgcatta acaggaattt
    22681 ataatcgtgc tgagactgaa aggcagatta agaaatattt tgaaaaaaat ttaaatgtta
    22741 tgggtgcact gtttatgatt gatacagata attttaaaca gattaatgat acagaagggc
    22801 atatgattgg agatatagta cttactgaga tggcatcagg tatgaaaaaa ataatgcgtg
    22861 acagtgatgt tgttggaaga ataggtggag atgagtttac aatttttatg aagaatattt
    22921 cctcagttaa agatgcagaa aagaaagcag aagaactatt gtatatgttc cgtcatctat
    22981 ttcagaaaga aaaaagtttt ctaaaagtaa cttgtagcat aggaatagct atatatccaa
    23041 aggatggtac tacattcaag gaaatatatg caagagctga taaagccctg tatcaagcaa
    23101 aaaatatggg aaaaaataat tatgtaatct acaaccaaga ttattttaaa gaattagaag
    23161 aatttgattg ttcttctctt ggtacagtta ttgattcaga aaaacaatac gctgaatatc
    23221 ctgataatct tacacgttat atattccgta tgctttatca gactgatgat atagaccagg
    23281 cgattaatat gatacttgag gttgttggga aacagtttga tgtaagtcgt gtttatattt
    23341 ttgaaaatac agaagatggt agatatactt ctaatactta tgaatggtgt aatgaaggaa
    23401 tctcttctca aatggcttat ttgcaaaaca gcaattatca agactatgag gattatgaaa
    23461 agctttttga ggatgaatta gttttttatt gtcgagatat ccatacatta agtcctaaac
    23521 tagaagaatt gttttctagt caaggtatcc actctacact tcaatgtgca tataaagaag
    23581 atagagtttt cagtggattt gttggatttg atgaatgcac aggactacgt ctatggtcac
    23641 aagaggaaat aagtacatta tcattgattt ctcaaattat atcaatgttt ttacaacgta
    23701 aaaaaaataa taaacttaat caacagattc aacaatacca gaatatttta gataatattg
    23761 accagtgtat ttgtgtgatt aataaagaaa ataatttttt attatatgta aataaaaaat
    23821 ttaaaaatac atattctaaa ttccaaattg gacaacctta ttatcttgat tctatagata
    23881 ttcataagat atccattata tggaataaaa aagaagcata cctatgtact agcatgattt
    23941 aatggtaaag taaagtttat attggactta aattaaattt tgttttaaat aagatattat
    24001 tttagttgat tactgaaaag actagaatct atattaatga attatactaa ttttgtacca
    24061 ctattaaaat attgtttgct aaaaataaaa aatagcctta aaacagttga ttataaaaaa
    24121 ttaactgttt taaggctatt tttatctttg tgacgaaata gacacttgcc atttagaatg
    24181 tgacaatata gtagttgttt agaaaaggca caaagcatta tactttaact aaagtaaaat
    24241 gtattttgct tttagaataa aaacaattag gagggagcta taattgaaaa agataatata
    24301 tgtattattt acaattgaac tattaatagt atctttatta ggtctaaata tagttaaaga
    24361 taatgaaatt aataatattt tatataacga tacaacaagt atatctgtaa tgtttaaaga
    24421 ttataaaagg ttaaataaaa attatagtaa gtggattaaa gatattgcag atgaaaataa
    24481 tgtgactata tcaaagtatg tttttaataa taatgaaaaa ttatctatat atacgactga
    24541 tacaagtttg aataacaata taaagttaaa atcaggaaaa tttcctaata ctaattcaaa
    24601 tgaatttatt agtaatataa acactaataa tagtaatcaa gtaggtaaat tctcaactat
    24661 gagtaaagac atatcaattt taattaggga ttttggtaaa ataagtgaag ttggagaatc
    24721 aggaatctta tatatttcaa ctcaaaatga agatacaata aataaaatat taaaagagtt
    24781 aaatgtggac agaagtattt ttatagcaag attacatgat agatatgtta actcaaattt
    24841 atacttaaat ccttcaataa ttagagattt aatactaatt atcctttgtt tcttggctac
    24901 aataatacat tattcaatta gtgtgagtag agaggcttct attttaagat taaatggata
    24961 ctctaagcta gatataattg ctagagttat aaaaaatata cttagaatta tgattttatc
    25021 atctgtcaca gcattattaa tatatataat ctatatttta aaattaaata taggtgttag
    25081 agcatgctta tactttgcta tgttttcgat tatatgtatt ttgataaata ttttgatatc
    25141 tatattaata gttggtttta atagtagaag ttctaaatat atgctatctc taaatggaaa
    25201 aaaatcttat ggttttgtaa atataatgca tttcacattg aaaattgtat ttatatcatt
    25261 tcttatatta tccataaata actgtatttt aaattataat atgctacaaa ctcagttagg
    25321 aaatctatct gagtgggata aagcaaaaaa tgtttataat cttactctta aagatacagg
    25381 tgaacaatct ttaggaaagg aagaagtaat taaaaatgcc aagattaaag gtttctataa
    25441 aaatttggta gaagaaaaag atgctttctt aatagattca actaattttg aaaaacttga
    25501 agatggaagt tacatgtatg aaataaattc aaagggtaaa aacccagaag taagtcaata
    25561 tggtaaaaat ataaaaataa ataaaaacta tcttaaagtt aaccctattt actcaaatgg
    25621 caaagaaata tttaatctaa tcgattatga cgataagact ataaatttat tagttcctaa
    25681 aaagttacaa gtacatgaaa atgagattaa aaaagagttt agagaattat tttattttga
    25741 aaaaatagaa gtagaaaata tgtacaataa agagttaaat agagagttaa ataaaacaaa
    25801 gaagtcagat ttaaatgtta atataatata tgtagataat aatcaaagct attttactta
    25861 taactccctt gttatggatg acaataggaa tctcattgat gacccaatag caatagttga
    25921 catagataat attgatgatt ctttttatct ttcttatata tctagatgtg tatattttaa
    25981 ttctaagaaa ttagatgccc ttgcagatat aagtaacatt attgaagctc aaggtgtaga
    26041 agcgcatatc caaagtttac atgctgttta taatgagtat ggtttagaaa taaataaact
    26101 agaaaaatat ttgaatagtg aaatttttac tataataata atagcaatat caaacttaat
    26161 gattacctac aatatagtag ctagctacta tgaaagaaat aaatataagt tatatattaa
    26221 aaaaatattt ggttattcag ctacagctag aagtatgttg ttaatagtct cattaatatt
    26281 aacaaacatt attccaatag gcattatatc aacaagagta gatttaagta ataatattat
    26341 cttatttgga cttctaatat tagttataga agtaattgta tctattgtat tagacaagat
    26401 tatctcaaat agttcattta ataaaattat aaaaggagaa cattaaaaag atgatttcat
    26461 taataaatat atctaaaaaa tttaaagata aatctataat agacaaattt aacctagaaa
    26521 tttctaaggg cgagtttgta gcgataacag gaaatagtgg ttcaggtaag acaactttac
    26581 ttaatataat gggattatta gaaaaaccta attctggtga tattataata aataatataa
    26641 aaaatccaaa tagtaaacag ataagaattt tacaaaggga tttttatggc tatctatttc
    26701 aaaattatgc tttaattgag aatgaaaatg ttgaaaataa cttgaaaatt gctttaaagt
    26761 atcaaaaaaa tatcaaccat aatgaaaaaa taaattacgc acttgagtct gtaggtctta
    26821 gaaaatacaa taaaaaaaag atatacgaat tgagtggagg agaacaacaa agggtagcat
    26881 tagcaagagt aatattaaaa gaatgtgaag taatatttgc agatgaacct acaggtaatt
    26941 tagataaaaa aaatagagac atagtatttg atatacttaa aagtctaaat aagaatggta
    27001 aaactatcgt atttgtaact catgatttag agttatcgga gcaggcagat agagttataa
    27061 aattgtaata atatcaacta taataaatat aagaaagaga ttacagctaa atcaagttta
    27121 ataataatac gaatagaatt taaacattaa gtaaattagt agtttgtaaa tataagtata
    27181 atatgatatc ataaaggctc aaaagaaatt ttatataata agaaatctaa aaataacaaa
    27241 tggaggacat ataaagatgg aaaatttacc aaattgcccg aaatgtaatt cagaatatac
    27301 ttatgaagat ggaactcttt tagtttgtcc agaatgctct catgagtggg cattaggatt
    27361 gggagctgaa gaagattcaa atattatcaa agatgtaaat ggaaatgtat taaatgatgg
    27421 agattctgtt acagtaatta gagaccttaa agtaaaagga agttcatcat ctataaagat
    27481 tggaacaaaa gtgaaaaata tccgtttaat ccatgactca tcagatggac atgatataga
    27541 atgtaagata gatggttttg gagctatgaa gctaaagtct tctgttgtta aaaaagcgta
    27601 atattataaa taagctgtag tgtttattgt ttttcagtac agtataaaac cataagaatc
    27661 tattaagtaa taaaagtttg tttatttatc tttatatttt cttatggttt tattaaatat
    27721 aaattttatt gaacaccaaa atattctctc ctctacaaat aagccaatct tatttaaact
    27781 aatatacttt gtactattta tactgaaatt gtaaatttaa atgctatgtt atttaggttt
    27841 gtatagttaa tt
//
LOCUS       DAGSRM010000043        26575 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.043, whole genome shotgun sequence.
ACCESSION   DAGSRM010000043 DAGSRM010000000
VERSION     DAGSRM010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26575
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.043"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            143..760
                     /locus_tag="MJE33_002048"
     CDS             143..760
                     /locus_tag="MJE33_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HBY9647982.1"
                     /translation="MEIFAIGHSNYPYDKLINMIKKYDINCVVDIRETPYSKYNIQYN
                     KEAFNESLRNSGFIYIYMGKEFGAKRTNKDVYTQEGYADFEKVAKEDIFLNGIERLKK
                     GCQMGYRIVLLGAMQEPIRCHRSILVGKVLNKEGFDVKYIMHEGNLAYQEDIEESLLD
                     KYFSDRKQLSIDNLLGSALTREEMIQEGYKLANKEIGYRTEKLGK"
     gene            complement(917..1369)
                     /locus_tag="MJE33_002049"
     CDS             complement(917..1369)
                     /locus_tag="MJE33_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staygreen family protein"
                     /protein_id="HBY9647983.1"
                     /translation="MVNLNANNLNVNMVYPISSTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTLGLGTMNIDKVYGQWAWFTDNIYDLNLFVFVGNYPYQIAKNRYDTFVDILPVA
                     ITSIVNGDRVFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
     gene            1817..3175
                     /locus_tag="MJE33_002050"
     CDS             1817..3175
                     /locus_tag="MJE33_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBY9647984.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTMMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDSEKVNETIKQALVSGVILSFTITILM
                     WVFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIATQQIANGILRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGIDLNPLGINLQTPSYGITGAAISIAIARIIGCIVIIRAL
                     FKGNEFISMGKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFIV
                     GAIFIPIAPWISSMYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGALYCLRLRGTKWLKHK
                     VA"
     gene            3448..3828
                     /locus_tag="MJE33_002051"
     CDS             3448..3828
                     /locus_tag="MJE33_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBY9647985.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMKQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMPYGRMFSFKDQDGNDYLVREDK"
     gene            3953..4153
                     /locus_tag="MJE33_002052"
     CDS             3953..4153
                     /locus_tag="MJE33_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9647986.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            4349..5077
                     /locus_tag="MJE33_002053"
     CDS             4349..5077
                     /locus_tag="MJE33_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBY9647987.1"
                     /translation="MLEIKNLSFNVESNNEELGIINDVSLSFERGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESNQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIMCQVNSSCELMKDMN"
     gene            5099..6016
                     /locus_tag="MJE33_002054"
     CDS             5099..6016
                     /locus_tag="MJE33_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HBY9647988.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTEAGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGEGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVILEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTEEEAEQQILNCFLK"
     gene            6158..7342
                     /locus_tag="MJE33_002055"
     CDS             6158..7342
                     /locus_tag="MJE33_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBY9647989.1"
                     /translation="MKNLLIRNGTIISPTDGIKFEKRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPSNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTSLDVINFDKVESIVEKNI
                     EKIKGIKVKASKFQSNESGIDIIKKSKQVAKKINLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKESGTLRKSVINAKNRGVLFDVCHGVDQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLNVINKIMELGISLEKCIEKVTEYPTKIFDLHGNKGRI
                     KIGGEGDFTIFTMEECNDIIKDYNNNELVLNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            7449..7637
                     /locus_tag="MJE33_002056"
     CDS             7449..7637
                     /locus_tag="MJE33_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBY9647990.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPEDLQ
                     DLCKEAEEGCPVSAITVK"
     gene            7786..8436
                     /locus_tag="MJE33_002057"
     CDS             7786..8436
                     /locus_tag="MJE33_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBY9647991.1"
                     /translation="MKKNYEIVLFDLDGTLTDPKEGITKSIQYSLNSFGIKEDLENLD
                     QFIGPPLHDTFKEYYKFEDKKAKEAVEKYREYFADKGIFENKIYENMKEILEMLYKNG
                     KILLVATSKPTVFAETILRYFDIDRYFKYIAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEEISESEPTYIVENVESIKDILL"
     gene            8539..9477
                     /locus_tag="MJE33_002058"
     CDS             8539..9477
                     /locus_tag="MJE33_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBY9647992.1"
                     /translation="MKSILIMGGSDFIGSALAKRLIKCGYQIDILTNGKKEIDYNGFK
                     EHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDNLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFIINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEIIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            9599..10354
                     /locus_tag="MJE33_002059"
     CDS             9599..10354
                     /locus_tag="MJE33_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBY9647993.1"
                     /translation="MKKYNISENGALIKNDTIESKSESVKVVQISDTQIGSFYSTKNL
                     KKVANKINTLNPDIIVFTGDLIDYSNKNPSVDEITTILSSMNARLGKFSVFGNHDYMY
                     KLPRYYRHIMKNSNFNLLVNENKKIKLKDDKYINILGVDEILNGNPNIKYLESQIDNK
                     SFNLLLAHEPDLVDMLSKDTMNLVLSGHSHGGQIRLPIKGALVTPPYGRKYTKGFYDI
                     NGNHLYVSSGLGSTKLPFRFFNIPEIIEFNIII"
     gene            complement(10639..11373)
                     /locus_tag="MJE33_002060"
     CDS             complement(10639..11373)
                     /locus_tag="MJE33_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBY9647994.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     SYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMVYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
     gene            11498..12214
                     /locus_tag="MJE33_002061"
     CDS             11498..12214
                     /locus_tag="MJE33_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBY9647995.1"
                     /translation="MKKFKLIGAIILVVFISVFISKDRLLKKDKDPIVAAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            12348..13040
                     /locus_tag="MJE33_002062"
     CDS             12348..13040
                     /locus_tag="MJE33_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY9647996.1"
                     /translation="MNNTVLVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKK
                     ENIHLMILDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFSGANNEDKDVIKVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            13030..14211
                     /locus_tag="MJE33_002063"
     CDS             13030..14211
                     /locus_tag="MJE33_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBY9647997.1"
                     /translation="MKNKSLLNHMFRPILDLIISCALTSMLILTSMYIIKSLYVNSDM
                     SVVNQLVYQARDFRSLIGYDLFYPFLFTITSLAIYSMISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKKEDMAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHDGEITVSSDKYQTVFEVQLPIIQS
                     A"
     gene            14493..14948
                     /locus_tag="MJE33_002064"
     CDS             14493..14948
                     /locus_tag="MJE33_002064"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBY9647998.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAIECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            14938..15663
                     /locus_tag="MJE33_002065"
     CDS             14938..15663
                     /locus_tag="MJE33_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vitamin B12 dependent methionine synthase,
                     activation domain protein"
                     /protein_id="HBY9647999.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIKINKDNVVELEGTNLTLESKDVYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLKQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            15635..18016
                     /locus_tag="MJE33_002066"
     CDS             15635..18016
                     /locus_tag="MJE33_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HBY9648000.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDKAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDKCVVCSPSKFVEVQSPTVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSYGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNVTLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(18221..19450)
                     /locus_tag="MJE33_002067"
     CDS             complement(18221..19450)
                     /locus_tag="MJE33_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBY9648001.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWSIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
     gene            20051..20965
                     /locus_tag="MJE33_002068"
     CDS             20051..20965
                     /locus_tag="MJE33_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648002.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGIKTEEDDENKIENPYIIIAF
                     GYPAKNEELFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEETQEENVTLEKEESQDE"
     gene            20989..21624
                     /locus_tag="MJE33_002069"
     CDS             20989..21624
                     /locus_tag="MJE33_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HBY9648003.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     RKIAYNAGLVEAEGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTVQKIFEHH
                     GFEVFSVICKNGAMPKSTIGLEASQTISKCSDEVMCNPIGQALFLNEAKVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKDEE"
     gene            22000..22716
                     /gene="pyrF"
                     /locus_tag="MJE33_002070"
     CDS             22000..22716
                     /gene="pyrF"
                     /locus_tag="MJE33_002070"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBY9648004.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVKAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGTDFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVREIENA"
     gene            22742..23785
                     /gene="carA"
                     /locus_tag="MJE33_002071"
     CDS             22742..23785
                     /gene="carA"
                     /locus_tag="MJE33_002071"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBY9648005.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKEAIGEVVFNTSMAGYGELLTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGTGAKVVVIDFGVKNSVLESLKACNCDITIVPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEVTHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            23838..>26575
                     /gene="carB"
                     /locus_tag="MJE33_002072"
     CDS             23838..>26575
                     /gene="carB"
                     /locus_tag="MJE33_002072"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBY9648006.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEVADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIADNEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVANELGYPVLVRPSYVLGGQGMEITYNEEKLSQYLQDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEV"
BASE COUNT        10752 a         2772 c         4749 g         8302 t
ORIGIN      
        1 tttttaagga tataaagtaa aaaaatatac aatatcaaga aaaaaagata ttgggtattt
       61 aaagtaatac tttatttaat tttaagtaaa tcgggtatct acatatatag atactaattt
      121 caaagaatag gagggtgttt atatggaaat attcgcaata ggacattcaa attatccata
      181 tgataagctt attaatatga taaaaaaata tgacataaat tgtgtagttg acattagaga
      241 aactccatat tctaaatata atattcaata caataaagag gcatttaacg aatctcttag
      301 aaattccgga tttatatata tatacatggg aaaagaattt ggagctaaaa gaactaataa
      361 agatgtatat acgcaagaag gttatgcaga ttttgagaaa gtagcaaaag aagatatttt
      421 tttaaacgga atagaaagat taaaaaaggg ttgtcaaatg ggttatagga tagttttatt
      481 aggagctatg caagaaccta taagatgtca tagaagtatc ttggtgggaa aagtattaaa
      541 taaagaaggt tttgatgtaa aatatataat gcatgaagga aatttggcat atcaggaaga
      601 tatagaggaa tcattattgg ataagtattt tagtgataga aagcaactga gtattgacaa
      661 tttattaggg agtgcattaa cgagagaaga aatgatacaa gaaggttata agctagctaa
      721 taaagaaata ggatatagaa ctgagaaact aggtaaataa aaatatacaa gtagataaaa
      781 tacagtactt atcttaagta aaataatatt atgaatttat aatagctagg catgatgtgc
      841 caattaaaaa tattctagtt atttaataat atcattaaat ttagggtatt ttttaatagc
      901 acatcatgtt gcagttttaa aaaataaaat ctctaatatt gccataatca actgttctat
      961 taagagtagg atgactagat ataaatctaa ctgtaatttt tgaatttaac aaacaaggat
     1021 ttgaaattaa aaatactcta tctccattga caatagaagt aatagctact ggcaaaatat
     1081 ctacaaatgt atcatatctg tttttagcaa tttgataagg ataatttcct acaaaaacaa
     1141 atagatttaa atcatatata ttatcagtaa accaagccca ttgaccataa actttgtcga
     1201 tattcattgt gcctaaacca agtgtatcaa agtatgttgc tatagttaaa taaatatttt
     1261 ccatgttttc taaatatgaa acagtatatc ttctgaaacg tatgggtgct gtggaagaaa
     1321 taggataaac catatttaca tttagattat ttgcatttaa attaaccaaa gtatcacatc
     1381 ctaaaattag tctataaaat acaatattta aatggatgcg attttgttac atctaaagtg
     1441 taatatttta tatattgaaa aagtggtaaa agtttggatg attaaataat attataaaaa
     1501 atatataaaa tgtacttatt gttattgcta cacgatagtg atacaatatt aacgaaatat
     1561 agctactttt aataataacg gaaaatattt atattaaatt aataaatatt ttaaatgtgg
     1621 tatccataaa aaataataaa cataagataa ttataaagat atactgtgtt caaatgatat
     1681 taaacaagat ttatattatt tgaaagcagt atattataat gcgaacgata caattctaaa
     1741 ctttgcattg tcaatttaca gttcgataaa ctaattaaaa attaaattta tataaagagg
     1801 gaaaagagga atagggatgt ttataaggaa aaatgttata aagttagcaa ttcctataat
     1861 gatagagcag acgtttgtca tgcttttagg agtatttaat acaatgatgg caggtcatat
     1921 tggggaagaa gctgtatctg cagttggaat ggttgattca ataaacaata tatttatatc
     1981 tgtatttgct gcactatctg ttggagctac tgttgttgta gctcaacaaa taggaaaaaa
     2041 agatagtgaa aaggtgaatg aaactattaa gcaggctcta gtgtctggag taatattatc
     2101 ctttacaata acaatattga tgtgggtttt tagaactggt gtaattaatt tattttatgg
     2161 ttcagcagaa gaattggtaa aatcaaatgc aaaattatac atagagttta cattatttac
     2221 gtatccattt attgcgacac aacaaatagc aaatggtatt ttaagagggt gtggagatgt
     2281 aaagaaacct atgtatgtga ctatgtttat gaatatagta aatgtaacat tgggatatat
     2341 acttatatat ggtattgatt taaatcctct aggaataaat ttgcaaacac catcctatgg
     2401 cataacaggt gcagctatat ctattgctat agctagaata attggatgta tagtaattat
     2461 aagagcttta tttaaaggaa acgaatttat atctatgggt aaaatatttc catttaagat
     2521 tgatgttgaa actcaaaagt ctatatttaa tataggaata ccagctggag ttgaacaact
     2581 actttttaat gcaggtaaat tgattgttca agtatttata gttactatgg gaacatcagc
     2641 aattgcatcc aatgcaatag gaatgtcaat tgcatccata ataaatgtaa ttgggaatgc
     2701 tttagctttg tctgctacaa ccttggtggg acagtatata ggacgtggag atattaaagg
     2761 agctaagagt actttattat atttaactaa atttgcaaca atttgtttat ttatagtagg
     2821 agctatattt ataccaattg caccctggat ttcaagtatg tatactcaaa atgctagtgt
     2881 aattgacatc tcaacacaat taattcgaag tgattgttta gctatggtaa tatggcctat
     2941 atcctttgta ttatcagcag ggttaaaggg agccggtgat actagatata caatgatgac
     3001 tgcagtaata ggtatgtgga tttttagaat ttttacaggt tacgtattgg gggttgtatt
     3061 agaatttgga gtgttaggaa tttggatagg tatgtatact gattggcttg ttagaggggc
     3121 tttatattgt ttaagattaa gaggaacaaa gtggctaaaa cacaaagttg catagaatat
     3181 atgaaatcac ttttagatgg ataactctat ttagaagtga ttttttatgt agttattatc
     3241 ataattataa gtatgatata taaggtgatt aaattacata aataaaggct ataatagtat
     3301 ggatattata aattatatat tgagcagaat tacataattt ggcgataaat attagaaata
     3361 tatatctata gaaaataata tatatgggta tattattaaa tataaaaaat attaggtatt
     3421 tatttaataa taaaggtggg tattgttatg ataaataaaa taggaaaaat aactctctat
     3481 gtaaataatc aagaggatgc aaagttattt tggacagaaa aattgaactt tgtagtaact
     3541 tatgaaaagg aaatgggtcc aaatatgaaa tgggtggaag tagcaccaag taaaaatgaa
     3601 tttacaacat ttgtgttgta tgataaaaat ctaatgatga agcaaaacaa agagataaat
     3661 gtatctcatc catcaataat tttaagtaca agagatatta atggaactta tgaacaaatg
     3721 gcaaataacg aggttgaggt tggggaatta atggagatgc catatggaag gatgttttct
     3781 tttaaagacc aagatggaaa tgattatttg gtcagagagg ataaataatt ttacaaagta
     3841 ttaaatttac tcaatatctg atataattag agacaataat tattataaac tagtctagga
     3901 ttagaattat ataattaaaa attaagtgta aattttgagt agaggtgata aaatgtttaa
     3961 gaaaatggca gtactaaaag atatagcaac taaaataggt cgtaaaaaag cgtatgaact
     4021 attagaaatg gttgaaggta atgatgcctt tgtagctgag gtaaagataa aaaagaatgg
     4081 aatagaatct aaaaaagaag aaattatgtt gaaagataat caaaaaataa tattagagta
     4141 tatagaaggt taaattttaa atacatataa gttgatatac taaatttctg agatgagttt
     4201 acataaaact catctttttt acttttcaat aactttgttt tattatttaa tatacatttt
     4261 atctccttag agttgctgat atataaaaat tactgatata ataaaagaat ggatattaat
     4321 gtaatatttt atagaaaaga ggggtattat gcttgaaata aaaaatcttt cctttaatgt
     4381 tgagtctaac aatgaggaac taggaataat aaatgatgtt agcctttcat ttgagagggg
     4441 aaaacttatt gttatcacag gtccaaatgg tggaggaaaa tctacaattg ctaagcttat
     4501 tatgggaatt gaaaaagcta cgagtggtca gataatttta gatggagaag atataacaaa
     4561 tcttagtatt acagaaagag caaaaaaagg aataggatac gcatttcaac aaccaccaag
     4621 aataaaaggt atgacagttg aaaatttact tacacttgct catggaaaac cattgtctac
     4681 tgatgtatgt tgccaatacc ttacagatgt tggattgtgt tcaaaagact atttaaatcg
     4741 tgaagttgat aactctcttt ctggagggga aatgaaaaga attgagatag caactctttt
     4801 tgctagagat ttaaaggttt ctatatttga tgaacctgaa gcagggatag acctttggag
     4861 ctttggaaaa ctcaatgaaa gttttaagaa aatacatgaa gagtctaatc agactataat
     4921 cataatttct caccaagaaa gaatattaga gttagctgat gaaataattg ttctacaaga
     4981 tggttcagta aaaagccatg gaacaaaaga ggctatatta cctgaaatca tgtgtcaagt
     5041 taattcaagt tgtgaattaa tgaaagacat gaattaatta gaaaggaagg caaattaaat
     5101 gaacaaaata gatttaaatc ttttagaaaa agttgctgaa cttcgtggaa ctccattagg
     5161 agcatacaat atacgtaaaa atgggcaagg agtagagaga cattcaactg aaaatgtaac
     5221 tataattcct aaaactgata aacctggaat agatattata gtaaagccaa atacaaaagg
     5281 agaaaatgta catataccag taatagtaac agaggctggt gtacaggata tggtatataa
     5341 tgattttgaa attggagaaa atgcagatgt tgttataatt gctgggtgtg gtatacataa
     5401 ctgtggttgt gatgatagtg aacataatgg tatacataga tttttcttac gtaaaggagc
     5461 tcgcctaaaa tatgtagaaa aacattatgg tgaaggtgat ggagaaggaa agagaatttt
     5521 aaatccagtt actattgtca atatggatga ggattcatat tgtgaaatgg aaacaattca
     5581 aattaagggt gtagattcaa ctaaaagaga aatggatgca aacctaggta aaggagctaa
     5641 aatagttgta tttgagaaat tattaactca tggtaatcaa gttgcagaat ctaatatgaa
     5701 ggttatctta gagggtgaaa actcttctgc tcaagtagtt tctcgtacag ttgggcaaga
     5761 taattctaag caaaagttta atccttgtgt tattggggat tccctttgtt ctgcacatgt
     5821 tcaatgtgat tcaataatta tgggaaattc tcaaattagt gctatacctg aaattgctgc
     5881 aaatcatcca gatgctcttt tagtacatga agcagctatt ggaaagattg caggagacca
     5941 aatactaaag atgatgacat taggtcttac tgaagaagaa gccgagcaac aaatacttaa
     6001 ttgcttctta aagtaaatta aagattttat ttaatttaat ataattaata tagtataata
     6061 tagtgaataa gtctaaaata tttttatttt taggcttatt ttctttatgt aagaaaagct
     6121 attagagaaa ttatattgta attaggatgt ggaataaatg aaaaatttac ttataagaaa
     6181 tgggacaata atatctccaa cagatggtat caaatttgaa aaaagagata tcttggtttt
     6241 acatgggaaa atatatgcta ttggattgaa ccttaaagag aaaataagaa aagaaaaatt
     6301 acttacaaat aaagagttcg agataataaa tgccgataat atgtatattt ccccaggatt
     6361 tatagatgtt cacacccatt gttataagag aaaattacct acaggtatgg attctgattc
     6421 aattggaatt gaaaaaggtt ctacagttat atttgatgca ggtacagctg gtccttcaaa
     6481 ctactatgat tttaaaaaaa agtatatgga tgagtgtaaa acagaagtat attctttttt
     6541 aaatgttaca aatgagggac taaatatatt gaatgaatca acttctttag atgttataaa
     6601 ttttgacaag gttgagtcaa ttgtagaaaa aaatatagaa aagataaagg gtataaaagt
     6661 aaaagcgagt aaatttcaat ctaatgaaag tggtatagat ataattaaga aatcaaagca
     6721 agttgctaaa aaaataaatt tacccttagt ggtatatata ggagagtatc catcatatat
     6781 agatgaagta atgaatattt tgggaaaagg agatgtggtt acaccagtat atggaaatag
     6841 tacaaatgga atccttaaag aatctggcac attaagaaaa tcagtaataa atgcaaagaa
     6901 tagaggagta ttgtttgatg tatgtcatgg agtggaccaa tttgatttta aagtttttaa
     6961 aaaagcaata aagcaaggtt tagagcctga cctaataagt actgatatgc atataaaaaa
     7021 catgaaagac gtaaactatt ctcttttaaa tgttataaat aaaattatgg aacttgggat
     7081 atccctagaa aagtgtatag aaaaagtaac tgaataccca acaaagattt tcgatttaca
     7141 tggaaataaa ggcagaataa agattggtgg agaaggtgat tttacaatat ttacaatgga
     7201 ggagtgtaat gatattataa aagactacaa taataatgaa cttgttttaa ataaaaaatt
     7261 aagattgcaa tatactgtaa aatcatggat gaaaagcagt gaagtttata gacatggata
     7321 cgaaaatact ataataaact aaacaatagt gataaaataa taaatatatg tttaaattgg
     7381 tatatattgg ataaatatct aaggaaacaa caattaaaaa ataataattt taggggaggt
     7441 attttagtat gaaagcattt gttgataagg atgtatgtgt agcttgtgga gcatgtatag
     7501 gaacatgtcc agacgttttt agtatgagcg atgaagggca ttcagtagca attgaaggag
     7561 aaataccaga agatttacaa gatttatgta aagaagccga agaaggatgt ccagtttcag
     7621 ctataacagt taaatagagc tgtttaaatt tatttttaaa ataaatatat aagatttaat
     7681 tggtacttaa gctatagtat tagaaatttc tattactata gctttttaaa atagataaac
     7741 ttaatatata agatattact ttttacaaat atggaggact gaaatatgaa aaaaaattat
     7801 gaaatagtat tattcgattt agatggaaca cttacagacc ctaaagaagg aataacaaag
     7861 tctattcagt attctttaaa tagctttggc ataaaagaag atttggaaaa cttagaccaa
     7921 tttataggac caccattaca tgatactttc aaggagtatt ataaatttga agataaaaaa
     7981 gctaaagaag ctgttgaaaa gtatagagaa tattttgctg ataaagggat atttgaaaat
     8041 aaaatttatg agaatatgaa agagatatta gaaatgctct ataaaaatgg taaaatatta
     8101 ttagtagcaa cttctaagcc aacagttttt gcagaaacta tacttagata ttttgatata
     8161 gatagatatt ttaaatatat agcaggtagc aatcttgatg gtacaagagt gaataaaaat
     8221 gaagttatac aatatgtact agacctatgt aatgtaaaag ataaagataa agttattatg
     8281 gttggagata gaaagtatga cataattgga gctaaaaaaa taggtataga ttctattgga
     8341 gtattgtatg ggtatggttc ttttgaagag attagtgaaa gtgaacctac ttatattgtc
     8401 gaaaatgtag agtctataaa agatattttg ttataaatat cttttataga caaaaatatt
     8461 acaaaatggt gtcgcatatt taaaaatatg atattatagt taaggaagtc atttaaaaaa
     8521 gaggaggatg cacttaaaat gaaatctatt ttaattatgg gtggaagcga cttcataggt
     8581 tctgcacttg ctaagcgctt gattaaatgt gggtatcaaa ttgatatact tacaaatggt
     8641 aaaaaggaaa tagattataa tggttttaaa gaacatctaa tttgtgatag aaaagttcga
     8701 aaagatatgg aaaacattat cacaggaaga aaatatgatt acatatatga tatgacagca
     8761 tatacaaaag aagatgtaag taatctgatt gattttataa gcatggataa cttaaaaaag
     8821 tatatagtat tgtcagcagg agcagtatat aaagatagtg gaagaaatat aaaagaagaa
     8881 aatgagaaag gtgaaaatga aaactgggga aaatatgggt taaataaaaa agaggctgaa
     8941 gattttatta taaatagccc tataccatat ataattatta gaccaactta catatatggt
     9001 gaaaataata atttatatag agagtattat ttctttgaaa aaatagaaaa aaatgaaaag
     9061 ataccagtac caaaaggaaa acaagttagt aatcaattta tatatattgg ggatttggta
     9121 aaggtattgg aaagcataat gaaaaatcct catgtaagag aggcctataa tgtgacaaat
     9181 ccacagttaa tttcttggga tgaccttata tatacatgtg gagaaattat tggcaaagaa
     9241 cctataataa aatatgtaga tatggaaaaa gtagagttta gagaaagaac gtatttccca
     9301 tttagaaata tagactttaa tcttgatata aataagctta ttgaacatgg gctttatata
     9361 ccaaacgtgt tactaaaaga aggtcttaca gcaacatata aatggttttc agcaaataag
     9421 ccaaaaatgc atgacagaaa aatgaacaaa gttgagtctg taatgcaaat agtttaaaaa
     9481 aataaataag taggtagata ttttgaactc aaaaattaag tattttttaa aattttttat
     9541 cttaatattt attttgattt ttatatactc agttgcgata gagccgagtc tattaattgt
     9601 gaaaaaatat aatataagtg aaaatggagc attgataaaa aatgatacaa ttgagagtaa
     9661 atctgaaagt gtaaaagtag ttcaaatttc agatactcaa ataggtagtt tttattcaac
     9721 taagaatctc aaaaaagtag ctaataagat taacactttg aatccagata taatagtatt
     9781 tactggtgat ttaattgatt actccaataa aaatccaagt gtagatgaaa ttacaactat
     9841 tttatcaagt atgaatgcta ggttgggaaa attttcagta tttggcaatc atgattatat
     9901 gtataaactt cctaggtatt ataggcacat tatgaaaaat tccaacttca atcttttagt
     9961 aaatgaaaat aaaaaaataa aattaaaaga tgataaatat attaatatat taggtgttga
    10021 tgaaatatta aatggaaatc ctaatataaa atatctggag agtcaaatag ataataaaag
    10081 ttttaattta ttgttagcac atgaaccaga tttagtagat atgcttagta aagatacaat
    10141 gaatttagtt ctatctgggc atagtcatgg tggtcaaata agattaccta ttaagggagc
    10201 attagttact cctccatatg gaaggaaata tactaaaggg ttttatgata taaatggaaa
    10261 tcatttatat gtgagttcgg gtcttggaag tacaaagtta ccatttagat tttttaatat
    10321 accagagata atagaattta atattataat ctaacataaa atatttagaa aatataattt
    10381 tgctttttat aatatgtaga tgtatatact gtaaaaatgg attaaaccag tagattgaat
    10441 gaattaatag attaaattaa tatataaaat atttaagtta taattaatgt attagacaaa
    10501 ataaatacta ttaagaacta ttaaaatata gattaaagat aataacatat aataaataaa
    10561 aaagtaagat tgtgtagatt atataaagat tatttacaat taatacaatc ttgctttttt
    10621 atttaaaaat tttaattatt atttaaaatt ttttatcttc tctttatccc atataaacaa
    10681 tttctcagaa ttatttaaag caccaaatac atttctttta aaatcaggca tctctttatt
    10741 taaattagca ataagatttt ttatgtactc tctcctagta tgaccatttg cagggcaggg
    10801 atttgtgata ataggatagt tagaatcttt tgaaattttc ttagtcatat actcttctat
    10861 gtaaaccata ggtcttataa ctgttaaatc ctgtctgtct aggtatgtct ttggggaaaa
    10921 acaatttact ctaccctcat aaaacataga cattaaaaat gtctcaattg catcatcttt
    10981 atgatgacca agagctactt tattacatcc aagtttttta gcattatcgt tcaaagcacc
    11041 tcttcttaaa ttagcacata atgcacaagg attcttttct tgtcttatat caaatacaat
    11101 ttcttttatg tcagtttgaa attcataaaa aggaacattt atttctttac acaatttatc
    11161 tagtggagaa ttatcaacac ctccagtatt taatgttatg gctatcaatt caaagttttg
    11221 tggagaaaat ctttgataac ttttaagtat atgaagtaaa gttaaactat cttttccacc
    11281 agaaagtcca accgcaattt tatcattttc ttgtatcata tcaaaatctt gtatagcctt
    11341 acgagcttta cttagaagtt tttgtataga cattttatca ccatccttta aaaatattaa
    11401 atttaagatt tatttaagat ttacttataa aatacttaag tcttatactc taaaattaac
    11461 attatagcaa acataacaaa aaggagaaga tttaattatg aaaaaattta aattaatagg
    11521 agccattatt ttagtagtat ttataagtgt atttatatct aaagatagat tacttaagaa
    11581 agataaagat ccaatagtag ctgctcaaca tgattcagaa caagatgttg aggcaaattc
    11641 tcaaaataaa aatcactcaa atagtatatc aaatatatta ttggtaaaca agacaaatgg
    11701 aattagcaaa aactatactc ctgaaaatat tactaaggta aacatacctt ttgttgaaga
    11761 agcaacagag gaagaaaaac aaatggcagg agaacctgca aaggcagttg aagatttagt
    11821 taagcaagct aattcagaag gaatacagtt tttagggtct tcagcataca gatcttatga
    11881 tactcaactg gatacttata caagaagggt aaaatctcag ggacgagaaa aggcagatgc
    11941 atatgtagca aagcctggtt acagtgagca ccaaactgga ctttgtattg atttaacaaa
    12001 tccagaaaga tggtttgttg gaagtacaaa agaagctaaa tggttggcag aaaatgctca
    12061 caagtttgga tttataataa gatatcctga aggtaaagaa gatataacag gtacagcata
    12121 tgaaccatgg catattagat atgttggtaa ggatgctgcc gaggaaattt attcaaaagg
    12181 attaacttta gaagaatatt tacaaaataa ataatataaa tgtgtaaata atttgtatta
    12241 tattggaaat ttagaaatta ataaatttat agattacact aactaaaata tagtataatt
    12301 ttatatgaat aaagctatat acaaagatta agttaggatg tgtaaaaatg aataatacag
    12361 ttcttgttgt ggatgatgaa aaggaaataa gagaccttat agagatatat cttttaaata
    12421 gtggatataa tgtaattaaa gctgaagatg gaaaagaggc tctagatatt ttaaaaaagg
    12481 aaaatattca tttaatgata ttggatataa tgatgcctaa aatggatggg atagaagttt
    12541 gtagaaaagt tagagaaagt ttgaatatac caatacttat gttaagtgca aaatcagaag
    12601 acatggataa aatacaaggg ataatgactg gtgcagatga ctatttgaca aaaccattta
    12661 acccacttga acttactgtg agagtgaagt cgctgattag aagagcatat tattttagtg
    12721 gtgctaataa tgaagataaa gatgtcataa aggttggagt agtaattata gataaggaaa
    12781 aacatagtgt aacagtggct ggaaatgaaa taattttgac atcaagagaa tttgatatat
    12841 tatatttatt agcaaataat aaaggcagag tatatagtac agaagaaata tttgaaaagg
    12901 tatggaaaga gaaatattac caatctaata atactgtaat ggttcatatg agcagaataa
    12961 gggataaaat tgaaaaatat acagatggag aaaaaataat tcatactgta tggggagtag
    13021 gatataaaat tgaaaaataa aagtttatta aatcatatgt ttcgacccat attagatctt
    13081 ataataagct gtgctttaac ttcaatgctt attttaacaa gtatgtatat aataaaatca
    13141 ttatatgtca atagtgatat gtctgtagtt aatcaacttg tatatcaagc aagagatttc
    13201 agaagtctta tagggtatga tttattttat ccatttttat ttacaatcac atcattagca
    13261 atttattcca tgatatcgta caagagaaat aagcaaattg tagattttat aagaaagact
    13321 aatagaaaaa atcaaaaatt agaaatcatt aaaagtgctg taaatttaat ggatgaaggt
    13381 aacttagaaa aaagtataga tattgagtat gacttagata tattaaagga taaagaaaag
    13441 gatgatattg atgaactagc acataaaatc aataatatag tccatcaact tagaaatata
    13501 actattgaag agagacaagc tcagcagaca aaaacagatt taataacaaa tgtgtcacat
    13561 gacttaagaa cacctttgac atccataatg ggttatttag gagtgataga agaaggtaag
    13621 tataaagatg aagttcagat gatgtattat gttgatattg catatgaaaa atcgaaaaat
    13681 ttaaatgttc ttataaatga tttgtttgaa ttgactaaaa tgcaaaataa tacaataaaa
    13741 ttaaacaaaa tagaaataaa tttagtagag ttattaagtc aagtagtatc tcaatttgaa
    13801 atttatttta aacaagaatt tatggttagt agaattaatt ttaatgaaga aaaattaatt
    13861 gtaaaagctg atccaaataa attggtaaga gcatttgaaa atcttataac taatgctgtg
    13921 aaatatggaa aagatgggca ttatgtagat atagtcacag agaaaaagga agatatggcg
    13981 gttgtaaaag tgataaatta tggggaacca attccagtgt tagatttacc aaatatattt
    14041 gatagatttt atagagttga aaaatcgaga aatagaaatg ttggaggttc tggattgggt
    14101 cttgcgatta caaaaaatat tgtaaattta catgatggag aaattactgt ttcaagcgat
    14161 aaatatcaaa ctgtgtttga agtacaactg ccaataatac aatcagctta gttataaaat
    14221 aaaagtacga ttatgtgata taaatattat aacaataaag tgtttacaaa aataaaaaag
    14281 taatatataa tttaagtcag tttaataaga tacttatcaa gagttagggg agagagttag
    14341 ctcagtgatc ctataccaac cttttatcag gtggtcctgc taacaacgat gagatgactt
    14401 gaaataatat actcttctta tcaataatag ataggaagag ttttttttag aattaatgta
    14461 acaaatttta aagtataatg gaggattaaa taatggaaaa agtagaaagt tttaagctag
    14521 accatacaaa agtaaaagct ccttttgtaa gaaagtgtag tgtacttgat ggagtaaagg
    14581 gagataaagt aactaaattt gacttgagat ttttacagcc aaatgtagaa agttttggaa
    14641 ctgctgctat gcacggttta gagcatcttt tagctactta tttaagagat acattagatg
    14701 gtgtaataga cttatctcca atgggatgta gaacaggatt ttatttaata ctatggggag
    14761 atgtagatgc caaaacagtt aagattggtt tagaagaagc attaaaaaaa gttcttgaat
    14821 cagataaaat gccagcagct acagctatag aatgtggaaa ctacagagac ttatctttat
    14881 ttggggcaaa ggaatatgca aaagatgttt tagataaagg attttcttta aatatatatg
    14941 gagaataatt taattggaag agaagatata agcataaatc aaagtgaagt tttaagatat
    15001 ttacaataca gaggtcaaga gatagataaa gaccttaaat atactattga tgaatgtgta
    15061 gctataacta aaaataaaat aaatcctaga tatttatcta ggatttatcc tattaaaata
    15121 aataaagata atgttgtaga attagaggga acaaatttaa cattagagag taaagatgta
    15181 tatgaacttt taaaagaatg taatgagtgt gtaattatgg cagctactat agggatagat
    15241 atagaaaaag aaataagacg acactcttat tcaaatttaa caaaaggact aataattgat
    15301 tcatgtggaa caacagcaat agaagaagtg tgtgacattg ttcaaaataa tttagaaaag
    15361 gtttttttaa agcaagataa atttataact atgcgatata gcccaggata tggagattta
    15421 ccaatagaaa aaaatataga tatattaaac gttttgaatg ctcaaaaaca aataggtcta
    15481 acaattactt ctaatggaat tatgataccg agaaagtcag tagtggctat tataggtatt
    15541 tctaatacaa gaataaataa atctaagaaa gaaaagtcgt gtgaaaattg taaaaactat
    15601 agaaactgta catatagaaa aggagcgaac tcttgtggaa ataagacaat atctgaaaaa
    15661 taatatatta atatttgatg gtgcaatggg aacaatgctt caacaaaaag ggttaaagtt
    15721 aggtgaaaat ccagaagtat ttggtcttca aaatccagat aaacttatag aaatacatac
    15781 agcatactta gaggcaggtt cgaatgtaat tcttacaaat acttttggat gtaatgaact
    15841 aaaattagat agtaagtata ctgtagaaga agtgattgac aatgctgttt tggtggctag
    15901 aaaagctata gaaaatgtag ataatactaa accaagatat gtagctttag atattggtcc
    15961 tataggagaa atgctagagc ctatgggtac tttaagtttt gataaagcat atgaaatatt
    16021 taaaagacaa gtactacaag gcgttaaatc tggagttgat gtaatagtaa tagagactat
    16081 gatggattta tatgaagcta aagtagctgt attagcagca aaagaaaact ctgatttacc
    16141 tattttctgt actatgacat ttgatgaagg tggaagaagt tttactggat gtatgccaga
    16201 atgtatggtg gctactatag aaggtctagg tgtagatgca ataggagtta attgttcatt
    16261 aggacctaaa caacttttac caatagttga aaaaatagca tcaagagcta cagtccctgt
    16321 tatggttcaa gcaaatgcag gtcttccaaa tatagttgat ggagaggcta tatatgatgt
    16381 tgatgcaaaa gaattttttg aaggggttaa aaagttcgtt gaagttggtg ctactataat
    16441 aggtggatgt tgtggtacga atccaagttt tataaaagaa atatctgaga atataaattc
    16501 tgttacaaag ggatgtatcg agaaaattga taaatgtgta gtctgttcac catcaaaatt
    16561 tgtggaagta caatcgccta cagttgttgg agaaagattg aatcccacag gtagaaaatc
    16621 tctacaagaa gcattaaaaa atgaaaatgt tgattatgct ataaatttag gtttagaaca
    16681 agtaaatgct ggagcacaaa tacttggagt aaatgttggt cttccagaga ttgatgaaaa
    16741 aaaactaatg ccaaaattaa taagagagat tcaagcagta gtagatacac ctttacaagt
    16801 tgattcttcc aatgtagagg ccttagagca aggacttaga tattataatg gtagaacaat
    16861 agtaaactct gtaaatggca aagaggagtc tttagaaagt atattaccaa tagttaaaaa
    16921 gtatggttca tgtgtggttg gacttacact tgatgaaaag gggataccta aaaaggctga
    16981 agaaagattt gaaatagcaa aaaggatagt taatagagca gtttcttatg ggataaaacc
    17041 aaaagatata tttatagact gtttatcatt aactgtttca gcacaacaag aagaagcaat
    17101 agaaactata aaagctataa caatggtaaa gacactagga gtaaaaacta tattaggtgt
    17161 ttcaaatatt tcatttggtc ttccaaatag gaaagcactt aatgcttctt ttctaacatt
    17221 agctttaggg gcagggcttg atttagcaat tataaatcca aatgaatata gtatgatgga
    17281 agctatcaat tcttttaaaa tattaaataa cacagataaa ggatgtataa actatataaa
    17341 tcaatatagc aatataaata attcaaaaaa atcggattca tctacaaaga ttgataaaga
    17401 tttgcccttg gatattttag ttgaaagagg attaaaagat gaagcaaaaa atgtaacact
    17461 gaatctttta aaagagaagg atgaaaatta tatattagat gagattttaa ttcctgctct
    17521 tgataaagta ggaaaaagat atgatagtgg agatatattt ttaccacaat tgattcaatc
    17581 agcagaaacg gtaaaagttt cattaaatac aataaaggaa actttattaa gtaaaagtag
    17641 caataatgta tccaaaggaa aaataatagt tgctacagta aaaggtgata tccatgatat
    17701 tgggaaaaat atagtaaaaa taatgctaga aaattatgga tatgaagtta tagatttagg
    17761 taaagatgtc cctattgaag aagtagtgga agttgctaaa aagagaaata taaaattagt
    17821 aggattaagt gcacttatga ctacgactgt tcaaagtatg aaagatacaa ttcaggcact
    17881 gagagataat gagataaatg caaaggtatt tgtaggaggt gcagttttga cagaagaata
    17941 cgctgaagaa atgggtgctg attattattc aaaggatgca aagtctgctg ttgaaatagc
    18001 aaagttaaat ttttagcata taaattaaaa taaaggtttt actaaatagg atattttata
    18061 tataatataa atataagttt tagatactta tgtttaaaac aataggaatt aatctataat
    18121 aatgagatta attaatataa aaaatattta tcatcaacat taaaaagggc tgggaagtct
    18181 ggtataactt ccatcagccc tttttagtta aaatcatatg ttaaaaagcc caattaccat
    18241 ttttaaaaat ctgtatttgc tttccatcat aagtctcacc aataatatcc atatcagcag
    18301 aaccaatcat aaaatctaca tgtgttaaac tgtcatttac accatgttca tctaattctt
    18361 catcttttaa attatttcca ccttccagac aagtcttata agcagaacca attgcaaagt
    18421 ggcaagaagc attttcatca tatagagtat tataaaaaat tatatccgta ttggatattg
    18481 gtgagttgta tggaacaaga gcgacttctc ctaaataatg agagccttca tccgtttcaa
    18541 taagtttttc tagtgtatcg agtccctttt cagcagagaa atctattatt ttaccatctt
    18601 taaatgttaa gaagaaattg tcaattatgt ttccaccata aacaagaggt tttgtactat
    18661 aaacagttcc atttacttta aacttatgtg gcattccaaa aatctcttca gttggaatat
    18721 tcggattaaa acttattcca ttagggtctt tggatgctcc acttagccaa acatgaccct
    18781 ttggcaagtc tagagtcaaa tcagtttttg aggatttata ttttaaagtt ttaaagttat
    18841 ttttatttag aaagtccatt ttagatttaa gattttcatt atgttcttcc catgctttta
    18901 tagggtcctg accatctact cttgaacatt taaatatagc atcccacagt ttttctattg
    18961 ctttatcttc tgataaattt ggaaacactt tttttgccca atcttttgtt ggcacagaga
    19021 ctatactcca tttacattta tcagttagag tgtaagaacg ccattctttt aaagcttttc
    19081 cagatgcctt ctgaaaattt gcaactcttt cagggtcaac atcttttaat aaatctggat
    19141 tttgggcata gactgataaa aatgccccac cttcttttgc tatatctaca tattgttctg
    19201 ctgtccattt ggggaactca tgaaatgctt catctggagc atataagtat ttaataagag
    19261 tactttcttc atcagaccac tctatataaa catttttagc accacattta tatgcttctt
    19321 taagtgcatt tcttgcaaaa tcagcacatt ctattggtga ctttataagt agaatttgac
    19381 ctggctgtat gttaactcca acttttattg ctaattcagc gtatttttct aaatttctat
    19441 ttaaattcat atttatcctc ctatattttt aatattgaat aacttataaa gtattataac
    19501 attacttatg tttttaatat tgaacaactt ataaaatatt ataactttac ttagattttg
    19561 ggtcatattc aaaatattta tactttatag attgagaaat aaaaaaaata ttttaattaa
    19621 aacagattat gaggataaca aacttattta agcagtatgt tttttgttga taataatatt
    19681 aaagtagaaa taaaaatact tttttagtga aaagctatac aagaataccg aaaaaaactt
    19741 ataatataat aatgagatag atatttataa atgaaaaaaa atttttctgg caaagcttaa
    19801 ttaaaaagtt aatagctagt gttgttattt aaattagcat agaaaatcaa ataaaaaaat
    19861 tatgctaatg tatagacatt gataaggtag aatttaagcc ttttgaatgc tatattaaac
    19921 tagagtagat attcttatga atttcaaaat agggcaggag gaaaaaggaa ataaaactat
    19981 aaaaaatata aagtatttat ataaatttat agagtttata cgtcagaaga tttcagttag
    20041 gagggcataa atggagcttt atgaagccat tttttataga aaatctgtaa gaaatttttc
    20101 cagtaaaaag ataaaaaagc cattgatgga agaaataaaa agaagttgta aaaatttaga
    20161 atacttaaat gaagatttaa atattaaagc acatgttgtt gatagaggtc atataataca
    20221 ttttttgatg ggtaaggaat gtaaagtaaa ggcacctcat tatatagtag ttacatcaga
    20281 caaaggtgag gactatttac agaatatagg atttgctaca gaaggtgtag ttttgcgcct
    20341 tacaacattg ggaatagcta cttgctggtt ggaaggaaac ttaaagagag aagatatctt
    20401 ggaatttgta gatattggga ataagaattt tgaagaagat gaggaagatg atttagatta
    20461 tctagctgga ataaaaacag aagaagatga tgaaaacaaa atagaaaatc cttatataat
    20521 aatagcattt ggatatccag caaaaaatga agagttgttt aggacgagat atgctagacc
    20581 tgatagaaag ccagtaaaag atatgataaa taagataaat aggaatagaa agtggaaaga
    20641 tatcttagaa ttgacaagaa gagctccatc tgtaaaaaat tctcaaccgt ggtatttcca
    20701 taaggatgaa cgaggtcttc atttatttga aaagagacct aagaaacact gtgaagatat
    20761 gaataaggtt agcttaggtg tagccttaag acattttgat atagcttgta taaaaaataa
    20821 aatagatgtt tcatatgaaa aacttcctat aaggaataaa ataggaaaat catattttat
    20881 aactgtagtc gagcatgtga aaccagaaga agaaacccaa gaagaaaatg taactctaga
    20941 aaaagaagaa tcgcaagatg aataaaattt tggaagggga tattggatat gtataagtgt
    21001 ggaaaatgta atcttagagg ctgttctaat ggaaatatgg ataaattaat gaagatatgt
    21061 ccaactagag aaattgaact acaacaaaaa gcaaagcaac tttatgaaga agaagagaat
    21121 agaaaaatag cttataatgc tggattagtt gaagcagagg ggtattgtca atatactaga
    21181 ctaaaagaga ttatagagtt tgctaaaaag tgtggttata aaaagattgg catagcattt
    21241 tgtattggac taaaaagaga aatgcaaaca gtgcaaaaaa tatttgaaca tcatgggttt
    21301 gaagtatttt ctgttatatg taaaaatggg gcaatgccta agagtacaat tggactagaa
    21361 gcatctcaaa caatatcaaa gtgttcagat gaggtaatgt gcaatccaat aggacaagct
    21421 ttatttttaa atgaagctaa ggttgacctt aatgtaattc ttggattatg tgttggtcac
    21481 gatactttag ccatgaaata tatggaagct cctataacaa ctttagttgt caaagataga
    21541 gtaactgggc ataatccagt tgctgcaata tataatgcag aatcatattt tgagaaaaaa
    21601 ttgtttgata aagatgaaga ataattcaaa gacttattga cataaaaagc aattagtggt
    21661 ttaattataa aaaagataaa actttaaatt atcctataga tacataaagt tatataaata
    21721 aagaaaaata ttacagctaa attccttaaa gtagtccaga gagactaaag ggttatagat
    21781 aatgttagta tataacctct agtaaatctt ctagaggttt ttgatgagta taaaataaaa
    21841 atttaaatat aataaagtct tttaaactag tccagagaga ctgggaaaga tatctcatag
    21901 ctaatgaata gagttatgta tagataaaat ccttatgcct cttttggtgt gaggattttt
    21961 ttatgattat ttaggcttta ttaaaaagga gatgtaaaaa tgataaatgg taaagaaaaa
    22021 ttgatagttg caattgatac tgacgaattt gataaagcta aagaactaat agacagatta
    22081 gaagatagtg tagacatatt taaggtaggg ctagagcaat acgttgccac aaaaggtaaa
    22141 acaatagatt atcttaaaga aaaaggtaaa aaaatatttt tagaccttaa attccatgat
    22201 ataccaaata ctatgaaaag tgcagtgaaa gctgcagtga gagataatgt ttggttaatg
    22261 acaatacatg tatcagatat ggaaggaatg agacaatgtg cactaattgc tagggaagaa
    22321 gcagaaagat taaatataga aaagccatta gtagttggag taacagtttt aacttcatta
    22381 agcaaccaag atttacagga cataggatgt aatatgacaa cagaagagtt agctataaag
    22441 agagctaaac tggcaaaaga atcaggaata gatggggttg tttgttcagc gcaagaagta
    22501 gataaaatag tagaagcatg tggaactgat tttataacag tttgcccagg aataagacct
    22561 aaatcagcag aggttggaga ccaaaaaaga gttgtaactc catcagatgc aataaaaaaa
    22621 ggtgctcatt atttagttgt tggaagacct ataacaaaag cagaaaatcc aagagaatca
    22681 gctattaata ttgtaagaga aatagaaaat gcttagataa aatatttggg aggtagctta
    22741 catgaagggg aaattaattt tagaagatgg aacagtattt gaaggaaaag catttggata
    22801 tctaaaggaa gctattggag aagttgtatt taatacttca atggctgggt atggagaact
    22861 attgacagat gcaacttatt atggacaaat agttactatg acttatccac ttataggaaa
    22921 ctatggaata aatttagaag atgttgaatc aaataaggtg caagtaaaag gacttattgt
    22981 aagagaaaaa agtgataacc caaataattt tagatgtgaa atggatatag acacatatct
    23041 aaagcaaaat aaggtaatag gacttgaagg aatagataca agagcattaa ctaaaattat
    23101 tagaaacaat ggaactaaga gaggaattat aactttagaa aatgtatcat tacaagatgt
    23161 tcagtctaaa ttagaagagc attgtaacaa agattcagta aaaaatgtta caagaaaaga
    23221 aattgaaaat ataaaaggta ctggagctaa agttgtagtt atagactttg gtgttaaaaa
    23281 tagtgtacta gagtctctta aggcttgtaa ctgtgatata actatagtac cagcaatgac
    23341 tacagtagaa gaaattttaa atataaatcc tgacttaatc ttattatcta atggcccagg
    23401 aaatccagag gatataaatg atatagttga aaatataaaa aaattaatag gcaaaaagcc
    23461 attgacagga ataggtttag gacatcaaat tttagcattg gcattaggag gaaagacttc
    23521 aaagcttgta tttggacata gaggaggtaa tcacccagtt aaatgtttag aaactggaaa
    23581 ggtatttata acttctcaaa atcatggata taatgtttca gaaatgccag aaaatacaga
    23641 agtaactcac ataaatttaa atgacaatac tatagaagga atgagacata aggaattacc
    23701 aatatatagt gttcagtaca ttccagaata cacaacaaaa gaagatttag attgtatatt
    23761 taataaattc ttagcattaa tatagattta gtattattta cagatagtaa aaaccattta
    23821 gataaggaga aaaatttatg cctaaattag atagtataaa gaaaacttta gtattagggt
    23881 caggaccaat aataataggt caagctgcag agtttgacta ttcaggaaca caagcttgcc
    23941 agtctttgaa agaggaaggg attgaagttg tattaataaa tagtaatcca gccactataa
    24001 tgactgataa agaagtggca gataaaatat atatagaacc attaacaata gaatttatag
    24061 aaaaaataat agaaaaagaa agaccagaca gtttacttgc aggtatgggt ggacaaacag
    24121 ggctaaacct agcagttgaa ctttatgaaa aaggcatact agataaatat ggagtaaaaa
    24181 taataggtac ttcagtagaa tctatcaaaa aaggtgaaga tagagatata tttagagaag
    24241 taatgaaaga aataaatcag cctgtaatag ttagtgatat agttacagat ttacaagcag
    24301 gtcttgatta tgcacttaca attggttatc cagtagttgt aagaccagca tacacactag
    24361 gtggaactgg tgggggtata gctgataatg aggaagaatt aagagaaata ctttcacatg
    24421 gtctacaatt aagtcctgtt ggacaagtat taattgaaaa gagtataaaa ggttggaaag
    24481 aaatagaata tgaggtaata agagattcaa agggaaattg tatagttgta tgtaacatgg
    24541 aaaatataga cccagtcgga gttcatacag gagacagtat agttgttgct ccaactcaaa
    24601 ctttaagtaa caaagaatgt gcaatgctta aaaaagcatc tttagatata ttaaatgcag
    24661 tagaagttca aggtggttgt aacgttcaat ttgcacttaa tccacatagt tttgaatatg
    24721 cagttataga gataaatcca agagttagta ggtcatcagc tttagcatca aaagctacag
    24781 gatatccaat agctaaagtt tcatcaaaaa tagctttagg ttatacttta gatgagatag
    24841 aaaatgcagt aactaaaaaa acttatgctt gttttgaacc aactatagat tatgtggtgg
    24901 caaaaatacc aaaatggcct tttgataaat ttaaaaaagc aaatagaaaa ttaggaacta
    24961 agatgatggc aactggtgaa attatgagta taggaagtaa ttttgaggct gctattctta
    25021 aagggataag atcacttgaa acaggaaaat attcattagt tcacacacca tctgaggaga
    25081 gaagcattga ggaattaaag aaaagagttg tagtacctga tgatgagaga ctatttgatt
    25141 tagctgaaat gataagaaga ggctataaag tagaaatgat agaacaaatt acaggtatag
    25201 ataaatggtt tataaataaa tttaaatgga tagtagagca agaagaaaaa ttaaaagtta
    25261 tgaaaattga agatttaact aaagactatc ttcttgaatt aaagaaaaag ggattctctg
    25321 ataaaggtat atctgaccta atgaagataa gtccagaaaa attatatgaa ttgagaagtc
    25381 tatacaatat caaaccagca tataaaatgg ttgatacttg tgcaggtgag atagatgcta
    25441 tatctcctta ctattattca acttatgagc aatatgatga ggtagttgtt agtgataaga
    25501 aaaaggtgat agtattaggt tctggtccaa taagaatagg tcaaggtata gaatttgact
    25561 attgttcagt tcactgtgta aaatctttaa gaaaaatgga catagaaact ataatcataa
    25621 acaataaccc tgaaactgta agtacagact ttgatacatc agataagctg tattttgaac
    25681 cattaacaga agaagaagtt ttaagtataa tagaaaaaga aaaaccagaa ggtgtaatac
    25741 tacagtttgg tggacaaaca gctataaaat tggctaaatt tttacatgaa aaaaatatac
    25801 ctatactggg aactgatttt gatgatatag atgcagcaga agatagagaa aaatttgatg
    25861 atttactaga gagactagat ataaatagac caaaaggaaa gggtgtatgg tcacttaacg
    25921 aaggtattga agttgctaat gagttaggtt atccagtatt agttagacca tcttatgtac
    25981 ttggtggtca aggtatggaa ataacttata acgaagaaaa attatctcaa tacctacaag
    26041 atgcatttga tagagaccat aaaaatccag tacttataga taaatatcta actggtagag
    26101 aaatagaagt agatgctata tgtgataagg aagatatatt gatacctggt ataatggaac
    26161 atttagaaag agctggagtt cactctggag atagtacaac aatgtatcca agtcaaaata
    26221 tatctgatga aataaaagaa aagatagtag aatacactaa aaaaatagct ttagacctta
    26281 atgtgcttgg tatggttaat atacagttta tagaatttca aaatgaatta tatataattg
    26341 aggttaaccc tagagctagt agaacagttc cgtatataag taaagtaagt ggagtaccaa
    26401 tagttgactt ggctactaag tgtatgttag gagctaaatt aaaggattta ggatatggta
    26461 caggagttta caaagagcca aaattagtat cagtaaaagt accagtattc tcaatgtcta
    26521 aattatctaa agttgaagta agtctaggac ctgaaatgaa gtctacagga gaagt
//
LOCUS       DAGSRM010000044        26481 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.044, whole genome shotgun sequence.
ACCESSION   DAGSRM010000044 DAGSRM010000000
VERSION     DAGSRM010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26481
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.044"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            160..1824
                     /locus_tag="MJE33_002073"
     CDS             160..1824
                     /locus_tag="MJE33_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBY9648007.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERMKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIDIFKEEVGLSIEEIFLEFKEEPIAAASIGQVYEGVLKTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKVYSEYNTKKILVMEKVNGTKLSDVEKIRRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVIAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLIEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNVEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            1837..4287
                     /gene="recJ"
                     /locus_tag="MJE33_002074"
     CDS             1837..4287
                     /gene="recJ"
                     /locus_tag="MJE33_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBY9648008.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKLAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYNVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQSEIPSAFAVINPKQEDCNYPFDSLCGCGVAFKMIQALTPKEEFKTSMYN
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILEEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLGIENGEATGSARS
                     IKGFNIFEALIKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NVNVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     SIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKDTLIISNSIN
                     GFYRAMSDISLIDLDFNINYNIIEDVSKNTDKLELIFSPNIDKIDLKRYNNIILYDYL
                     YNKGEYSYIYENKREESEIIKYYNKTDLLYLKNVVSNIVPSRDEFITIYKQALIKKEI
                     DLDMVNIRETFNVIPLKFFTILNVFRELNLLDFNLNYEKNSVLIRILPKPQKKLDLNE
                     SLILNNLKNLEKQYNSSY"
     gene            4300..4812
                     /locus_tag="MJE33_002075"
     CDS             4300..4812
                     /locus_tag="MJE33_002075"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBY9648009.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKDVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            4886..7093
                     /locus_tag="MJE33_002076"
     CDS             4886..7093
                     /locus_tag="MJE33_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003229747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBY9648010.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            7118..7567
                     /locus_tag="MJE33_002077"
     CDS             7118..7567
                     /locus_tag="MJE33_002077"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBY9648011.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNAARPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            7582..8196
                     /locus_tag="MJE33_002078"
     CDS             7582..8196
                     /locus_tag="MJE33_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBY9648012.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHVDEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVNDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGDFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            8287..9843
                     /locus_tag="MJE33_002079"
     CDS             8287..9843
                     /locus_tag="MJE33_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HBY9648013.1"
                     /translation="MLYVFLKGHDYKYEVAELIKLFTSEFKFKDSNANNNIESKHLIN
                     RLIYENGVLFSSTEYYENGNLKYESIQNIENMNLEFLDGFDSSKTIEEFLNSLDSESK
                     RNFKKLCKENIKKSMFVVLKQVFNSYVPWGILTGIRPVKIVHNLMDKKLDDNSIRTIL
                     KDNYFIIDEKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLKQ
                     FGHLKREYVYKLIEEIKGFAKVIKETKKEVETLYIGGGTPTTLDEEELDLLINSLFNE
                     LDLSKIKEFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDDTLVKIGREHRVSD
                     LVDCFNMARRMGFDNINMDIILGLIDEDLNMVKNTLEEIKKLSPESLTVHTLAIKRAS
                     NLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAKE
                     GYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIKR
                     KKEEVYKNVD"
     gene            9833..11095
                     /locus_tag="MJE33_002080"
     CDS             9833..11095
                     /locus_tag="MJE33_002080"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBY9648014.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEKANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            11169..12956
                     /gene="aspS"
                     /locus_tag="MJE33_002081"
     CDS             11169..12956
                     /gene="aspS"
                     /locus_tag="MJE33_002081"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBY9648015.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKEAFEKAEKLGAEFVIAVKGKVCERQSKNPNMPTGDIEIFA
                     TELRLLNKSETPPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEAIFKEVLDVELALPL
                     PKMTYAEAMSKYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFNEEEMNAILTRMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKSSLRAKAYDIVLNGYEIGGGSVRISNSDVQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGTDNIREVIAFPKNQNA
                     VCPMTNAPTLAEDEQLEELSIKVDIKDNE"
     gene            13415..14647
                     /locus_tag="MJE33_002082"
     CDS             13415..14647
                     /locus_tag="MJE33_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBY9648016.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMILMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFAASYYSSKINFLIPPINGIV
                     VSMVIYYILMNCFGIKDK"
     gene            14661..15467
                     /locus_tag="MJE33_002083"
     CDS             14661..15467
                     /locus_tag="MJE33_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HBY9648017.1"
                     /translation="MGRKVKIGIIQQHSVLGNVKKNIEKAVEMIDDLGKQGADIICLP
                     ELFATGYNLESLGGVKTLELIREHNKYIEESMSEAAKRNNVYLISPYGTLEKGSTHVY
                     NSAVIFDRKGKIMGEYCKNHLWSLEAVYFKGGEKVEVYDADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKKYREDIPYLKDRKSSYNI"
     gene            complement(15579..16448)
                     /locus_tag="MJE33_002084"
     CDS             complement(15579..16448)
                     /locus_tag="MJE33_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBY9648018.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENSGEVKILSPAAAN
                     VLPSLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
     gene            16820..18247
                     /locus_tag="MJE33_002085"
     CDS             16820..18247
                     /locus_tag="MJE33_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HBY9648019.1"
                     /translation="MKLTKSISSMLCLSLVLSLSPVAYANEISRTTVENTLVGQDRYQ
                     TAVEVSKKGWSSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNNLNSQTEKELK
                     RLGVKKVYIIGLSSSVSNDVQKQLESNNLTTDRVGGSDRYQTALSIAKKIEGLKDISE
                     IAVVNGYTGLADAVSIASVAATNGMVILPVSDASGISSFKDFIESKNINKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKNQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFSNKNATSITQVGSGGNENAFKELKDLQANS
                     NNKRVMYVTNTDKVNIRNDATIEASVIGYLNNGDEVEVLDVLKTGWVKIKYNEGIGYV
                     SGSYLTNNKPDNSNENIKIKYVKEKDGLNVRKGPSTEDEKMGQLSYGSKVETVEMFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
     gene            complement(18324..18593)
                     /locus_tag="MJE33_002086"
     CDS             complement(18324..18593)
                     /locus_tag="MJE33_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648020.1"
                     /translation="MKFEYYYLIQDIAGILLAFIGLRMSIIGFRILSMKGLSINTLLI
                     VIKYCLFTIAGLNLLISKFGIRHWIWSVCMLIISIIINPRIKVSK"
     gene            complement(18596..20038)
                     /gene="nhaC"
                     /locus_tag="MJE33_002087"
     CDS             complement(18596..20038)
                     /gene="nhaC"
                     /locus_tag="MJE33_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBY9648021.1"
                     /translation="MKMNTERKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAAMKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLIAAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICIVLYTVIGFKYSN
                     NTIDYVQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDVLNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLITKIGKSRTKLVGSTFIVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLEFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDDEIEELEIFDTAQRRVD"
     gene            complement(20063..21229)
                     /locus_tag="MJE33_002088"
     CDS             complement(20063..21229)
                     /locus_tag="MJE33_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBY9648022.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLYRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKANDKIMIQEPVYSPFNSVVKNNNRELIINPLQKLENGNYIMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPMAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVILPDEKDYKLLDDAFTRIDIKRNNCFSLV
                     ATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGL
                     SDEELESILVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNAIN"
     gene            complement(21454..23574)
                     /locus_tag="MJE33_002089"
     CDS             complement(21454..23574)
                     /locus_tag="MJE33_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY9648023.1"
                     /translation="MKQMELAIVSLKKDAGEIYENQIRQFLGDNLKINLYSFEEGNLK
                     FFKEKLILLSAYLKYDEIVKLSHYDAQIIVPKLTFEKNSIDMISKLEKDKIIYVYNLS
                     KDMAIETISLIHRLGIDNINILPCYPEIEFTPTDAVILTPGEKILPKFKNCEVIDLKY
                     RIIDLSCIVEIATKTKLKHLIKDDLIKKYVEKIIPTSYSTGELLLDANKFERQFDLLL
                     SIIDDGIICTNNDGIIQFYNHMARKILSINANEMIDSFVGDCIKDINFQSILTNKTPF
                     FEKLIKINHIDINLEIKHIQLNVFDGFILKMTKFSQLEKKQAKLRAQLVNSGNISKYT
                     FDDILGSSIQTINTKKIANKMAQSNSSILIIGESGTGKELFAQSIHSASRRKDGPFVA
                     VNCSTFQENLLQSELFGYDEGAFTGAKKGGKIGLFELANNGTIFLDEIGEMDLNSQSK
                     LLRVIQEKQVRRIGSNNVIDIDVRIIAATNRNLKELVSKNMFRRDLYFRLNVLPLKIH
                     PLRERPADIFEIFGSLKYDIPCNFILSEEVKEIFKMYRWEGNVRELRNLGEYFCYLEK
                     DIIEICDLPEYILDTIDSNYSRTVCNKVSDNIKKYQFNIGKDKNIMKYDYNFKRSLDE
                     YIFILDNLKKAYDLKERIGRKSLCKIALEENRFLTEQQIRNMLLELQDFGLVDILVGR
                     GGSIITSKGVEFLKNINRSNKLNS"
     gene            23807..24208
                     /locus_tag="MJE33_002090"
     CDS             23807..24208
                     /locus_tag="MJE33_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648024.1"
                     /translation="MKKCLYCNRDIKSPNNKLAIKYDDNMNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILAFIVIITVLYFKQVGK"
     gene            24251..24544
                     /locus_tag="MJE33_002091"
     CDS             24251..24544
                     /locus_tag="MJE33_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBY9648025.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            24559..24999
                     /locus_tag="MJE33_002092"
     CDS             24559..24999
                     /locus_tag="MJE33_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HBY9648026.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAVMAYIIPLIALLVGTIGTYYILGAIGMTTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(25091..25564)
                     /locus_tag="MJE33_002093"
     CDS             complement(25091..25564)
                     /locus_tag="MJE33_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBY9648027.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGVTGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(25564..26352)
                     /locus_tag="MJE33_002094"
     CDS             complement(25564..26352)
                     /locus_tag="MJE33_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBY9648028.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
BASE COUNT        10218 a         3147 c         4467 g         8649 t
ORIGIN      
        1 aactgagtta aataatttta ataaaattgg tagtatatgt gataaaatta aatattaaag
       61 aggatatagg atattagaat atcaattggt gaaataatgt catatacgat aaattaaggt
      121 gtaagttttt gggtatacct atcttaggag tgaaaagatt tgagaataag ttatagaaat
      181 ttaaagcgat atagagaaat tggatatgtt ttaataaagt atggttttag ttttattgta
      241 gaaagactaa atatagaagg aattgcctat aaaataccac tatttgaccc accagaagaa
      301 ataaaaaata tgacaactgg tgagagaatg aaaagagttt tagaagaact aggacctaca
      361 tatataaaaa taggacagat attgagtaca agaaaagact tgttagacca ggatataatt
      421 gatgaaatat caaaactcag agatgatgta gagaaatttg attcaaatat agctattgat
      481 atatttaaag aagaagtggg tttaagtata gaagaaatat tcttagaatt taaagaagaa
      541 ccaatagcag cagcttcaat tggtcaagtt tatgaaggtg tacttaaaac gggagaagaa
      601 gtaatcgtaa aaattcaaag accaaatata gagaaaataa taaaatctga tttagagatt
      661 cttagaacta ttgcaaatac tttaaaagat ttaaaaaagg attttaattt agatttagta
      721 cagatgattg aagaatttca aactcaatta atgagggaac ttgattatac atttgaagct
      781 ataaatgcaa ctaaattctc taggatattt aaaaatagtg atgaagtata tatacctaaa
      841 gtatattcag aatataatac taagaaaatt ttagtaatgg aaaaagttaa tggtacaaag
      901 ttaagtgatg tagaaaaaat aaggagacta ggttataaca caaaaactat tgttgaaata
      961 ggcgttaggt cattttttac acaggtatta tctcatggat tttttcatgc agacccacat
     1021 ccaggtaata tattcgtaat tgctaaaaat aagatagcat atatagattt tggaatgatt
     1081 ggaattattg ataataaaac tttaaaccaa ttaaatgaaa ttgcattagc aggtgtagaa
     1141 aagaatgttg acaagataat atatctattg atagaaatgg atgcattaaa tggagaggct
     1201 gatattaagg gacttagaca ggatttactt tatctaatac attattacta tgatatttct
     1261 atagaaaaaa taaatgtaac agatatatta aatgaactat ttaggttttt tagacagtat
     1321 aaaatagtca tgcctgcaca atttgtaact cttgcaaaga cagttataac attagaagga
     1381 acaagtagaa ctttaaacac agatttttct tttggttcta tgggaaaaga gtttatgaaa
     1441 catcattata agagtaaatt taaccctaaa aatgttgttt taagttcaag acaaaatgta
     1501 gaagaaatat tactggacat aaaaactatt ccaaagcaat taaaagcaat tttaaaaaat
     1561 atagaaagaa ataatataaa aatgcaaatt gaagatgtta aaatgacgag gttagaaaat
     1621 tgtataatag agctaacatc tcaaatatct ttgagtttag tgttggcatc tattatagta
     1681 ggttcttctc ttataattgc atctccaaat atagaaaata atatatggat aaaatttact
     1741 gcaatagcgg gtttctttat atcctttata ataggccttt gcttagttat aagaagtatt
     1801 aggtcaaaat acaaaaaaga ttagggggaa ttaaatttga aaaaatggat attgaagtat
     1861 aaaggtaaga tagaggaaag tgaatttagc aaaaaactta atatttcccc ggaaatctgc
     1921 caaattttaa aaaatagagg gatatgtact gaaaaagatt cagagatatt tatgaatcca
     1981 tctttagatt atttaagaga ccctttttta atgaaagata tgcaaaaagc tgttgataga
     2041 ataaagttag cagttgagaa gaacgaaaaa atttggattt acggagatta tgatgttgat
     2101 ggtgtatcat ctacatctat cttgtgctta tattttaaga gtataggtta caatgttgat
     2161 tactatattc ctaatagatt agaagaaggg tatggtataa atgaagatgc tattaagctt
     2221 ataagctcta ggggatgtga cttgattata agtgttgact gtggaattac atcagtgtct
     2281 gaggtcaata cagcgaatga tttgggcata gatgttataa ttacagacca tcatgaatgt
     2341 caaagtgaaa ttccatcagc ctttgcagta ataaatccaa aacaagaaga ttgtaattat
     2401 ccatttgatt cactatgtgg atgtggtgta gcatttaaaa tgattcaagc cttgactcca
     2461 aaagaagaat ttaaaactag tatgtataat tatttggaaa tagtaacact agcaactatt
     2521 tgtgatattg ttccacttat agatgaaaat agaataattg taaaaaatgg tttaaaatca
     2581 atgaaagaag gcaaaaatat tggattaagg gagcttataa aagtttgtgg tgtagaaagt
     2641 gataagatag gttcttctca tataggtttt gcaataggtc ctaggataaa tgcttcaggt
     2701 cgattgggat attcttactt aggagtagag ctatttacta ctcaatcaca agaagaggca
     2761 gtagaaatag ctagtatatt ggaagaaaaa aataatgaaa gacagatgat agaagctaag
     2821 atgtatcatg aagctgagga aatgctaaag tctaatagca gatacaatga tgacaaggtg
     2881 ttggttttag ctaaagaggg ttggcaacat ggaataatag gaattgtagc atcaaagtta
     2941 actgaaaaat actacaaacc aacaattctt ctaggtatag aaaatgggga ggctactggt
     3001 tcagcgaggt caataaaagg atttaatata tttgaagctc tcataaagtg caaagacttg
     3061 atgactaagt ttggtggtca tgaacaagca gcagggttat ccttggattc agacaatgta
     3121 gaaattttag caaatgaaat aaataaattt gcagattata atttaactga agatgatatg
     3181 atagagaatg taaatgtcga gtttgaatta caagaaaatg taataaattt aaatcttgta
     3241 gaagaattac ataagttaga accctttgga ttaaataatc ctaatcctag atttatagtt
     3301 agaaattata ttttaaaaga tttgaaagtt ataggtaaga atcaacaaca tctgaaatta
     3361 agtatagaaa aagagaaaag ctatgaatgt ataggattta atatgagtca tttaaaatca
     3421 atgtataaag taggagacaa agtagatgta ctattccaat tagatgaaaa taactatatg
     3481 ggaaatagga aagtacaatt tttacttaaa gatatacgat tagctcgacc taaaagtgca
     3541 agtaatgaca aactttcatt gaaattaatg tcaaaaataa taccaaaaga tactcaaagc
     3601 ctgtataata tatctgtgtc tgattttgaa ttatttgatg gaaatacaga tataaatata
     3661 tttgattatt ttgaaaaaga tacactaata atatctaatt ctataaatgg attttataga
     3721 gcaatgtcag atatatcact tatagatttg gattttaaca taaattacaa tattattgaa
     3781 gatgttagca aaaatactga taaattagag cttatatttt ctcctaatat tgataaaata
     3841 gacttaaaaa gatataataa tattattctt tatgattatt tatacaataa aggcgaatat
     3901 tcgtatatat atgagaataa aagagaagaa agtgaaatta taaaatatta taataaaaca
     3961 gacctattat atttaaagaa tgtagttagt aatatagttc catcaagaga tgaatttata
     4021 acaatttata aacaagcact cataaaaaaa gaaattgatt tggatatggt aaatataagg
     4081 gaaactttta atgtaattcc attaaagttt tttacgatat taaatgtatt tagagaatta
     4141 aatttacttg actttaattt aaattatgaa aaaaattctg tactcataag aatattgcca
     4201 aaaccacaga aaaaattaga cttaaatgag agtttaatac tcaataatct aaaaaattta
     4261 gaaaaacaat acaatagcag ttattaggag gcgtttccca tggatttaaa aaactttata
     4321 aggaatattg atgatttccc aaagccaggc atagatttta aagatgtgac aactctattt
     4381 aaagatggag atgcatttaa atatgcagtg gattctatag ttgaagaatt gaaagataaa
     4441 gatgtagatt tagtaatagg accagaagca agaggatttt tgatgggtac acctgtagca
     4501 tatgctttag gagtaggatt tgttccaatt agaaaacctg gaaagttacc aggtgaagtt
     4561 gagagttatg agtatggatt agagtatggt acagatactt tagagataca taaggatgca
     4621 attaaaaagg gtcaaaaagt agcaatagta gatgatttat tggctactgg tggaactatg
     4681 gaagccgctg ccaagcttgt agaaaaatta ggtggagaag tagtttctat gcaatttttg
     4741 attgagctaa aattcttaaa tgggagagaa aagttgtcta actatgatgt taattctctt
     4801 ataaaatact agtactttta taatagttgg tataacacca actattttca tatatattaa
     4861 aattttatac aaaataggtg gttatatgca tgataaagaa ttacaagaaa taattgaaaa
     4921 aataaaagtt tatgcaccta atgcaaattt tgaaatggtt gaaaaagcct ataatcttgc
     4981 aaaaaaagcg catgaaggac aatatagaaa atctggggaa ccatacttta ttcatccatt
     5041 agcagttgca aatattttaa tagagatgga attagatatt gaaactataa cagcaggttt
     5101 acttcatgat gttgtagaag atacagaata tacctatgaa gacataaaaa aagaatttgg
     5161 caaagaagta gcggatttgg ttgatggagt aactaaactt ggtcaaataa aatatcgttc
     5221 gaaagaagaa actcaatctg aaaatttaag aaaaatgttt ttggcaatgg cgaaggatat
     5281 aagggttata cttataaaac ttgctgatag attgcacaac atgagaacat taaaatacat
     5341 gcctccagaa aaagcaaagt ataaagcaaa agagacactt gaaatttatg gtggtatagc
     5401 tcatagatta ggaatttcca agataaagtg ggaactagaa gacagagcat taagatttat
     5461 ggacccagaa gggtattatg atttagttag tagagtatca atgaagagaa gtcaaagaga
     5521 ggattatatt caaggaattg ttgaacttct taaagataag tttaaagatt taagtatacc
     5581 atgtgatgta tatggtagac caaagcattt ttacagtata tatagaaaga tgcaaaaaaa
     5641 acataagact tttgaagaga tttttgattt aacagcagta aggatattgg ttgataatgt
     5701 aaaggattgt tatgcagtac ttggtatagt tcatacatta tggagaccta ttccaggaag
     5761 atttaaagat tatatagcaa tgccaaaacc caatatgtat caatcattac acaccactgt
     5821 tgtaggtcct gatggagaac ctttagagat acaaataaga actcatgaga tgcataatat
     5881 agctgaaaat ggtatagcag cccattggaa atataaagaa ggtatctcta gcaaagaatc
     5941 aaaggttgaa gaaaaacttc aatggttgag acagatgatg gagtgggaaa aagatttaaa
     6001 agacccacaa gagtttatgg atgcacttaa agaagatgta tttaatagtc aagtttatgt
     6061 atttacgcca aaaggagacg taattgaatt accagcagaa tcaacaccta tagattttgc
     6121 ctatagggtt catacaaatg taggtaataa atgtgtagga gctaaaattg atggaagaat
     6181 agttcctatt gattataagc ttcaaaatgg taatattgtc gaggtactta catcatctaa
     6241 ttctaatgga ccaagtaggg attggcttaa tatagtgaag accccaaatg caaaaagtag
     6301 aattagacaa tttttcaaga aggaaagaag agaagaaaat gttgaccgtg gaaatacaat
     6361 attagaaaga gaatttaaga agcatgatat accacaaaaa gaccctcttg ttgaaaaata
     6421 tatggcatta gttgctaaga aatttaatca gccaagtgtt gaggacttaa ttgccactgt
     6481 aggttatggt ggtataatgt catctcaagt ggtatctaag gtaaaagacc tatatttaaa
     6541 agaggttaaa aaagctaaaa aggaacaaca gatacaagaa gaagatttaa ataaacacaa
     6601 tataagtgaa acagaataca gtaaaaagag aaagaaaaac gcatcacaag gaattatagt
     6661 aaaagggctt gacaatatct tagtaagatt tgctaagtgt tgtaatcctc ttccaggaga
     6721 tgaaattgta ggatatatta ctaagggtag gggagtagcc atacacagag ctgattgccc
     6781 aaatgcaaac aataatggtg atttcttcca aaatagattg gtggaggttt catggagttc
     6841 agcaggaaaa ggtaagtttg aagcggaaat tcaaataaaa gctattgaca gaagaggaat
     6901 tgttaatgaa ataactcata ttgttgcaat ggataaactt agtttaaacg gaataaatgc
     6961 aagaaaaggt aaggatagta ttgtaagtat aaacttactt gtagaggtta atgacacaga
     7021 ggaattaaaa attctaatga aaaaattaaa agcaattcct ggtgtagaaa gtatttatag
     7081 aatgacaaat tagagttaaa ataataggga ggaaactatg agagcagtag tgcaaagagt
     7141 atcttcatct aaagttacag tagatgaaaa cactataggt caaataaata aggggttact
     7201 agtattgtta ggagtgactc atgatgataa atcaagtgat gtagattata tgattgataa
     7261 aatattgaat ctgagaattt ttgaagatga aaatgacaag atgaatttat ctctaatgga
     7321 tataggtgga gagcttttag tagtatctca atttacttta tatggagatt gcagaaaagg
     7381 aagaagacct ggatttagta atgcagctag accagaactt gcaaataacc tttatgaaga
     7441 atttgtaaaa aaagcaaaag ataaaggtgt gacagttggt acaggtcaat ttgcagctca
     7501 tatgatggta gagttaacta atgatggtcc agtaacaata ctattagact caagtaaatc
     7561 tttttaaggg ggaaaacttt aatgattata aaaaaatttg tagatagtta ttttggagta
     7621 aatacttatg tattaggtga tgaaaagact aaaaaatgtg cagttataga cccaggtgga
     7681 agtttagtag agacattaag ttttgtaaaa gaaaatgaat taaatattga atatattatc
     7741 cttactcatg ggcatggaga ccatatagga tatgttaagg atattaaaga aaaaactggt
     7801 gctaaagtag ttgcacatgt tgatgaaaaa gaattattaa atgacaaaaa taaaaatcta
     7861 agttatacaa tgagatgtgg agcacaagag tttgatgcag atatctatgt aaatgataaa
     7921 gacaagcttg aacttggtga attaaaaatg actttcttgc atactccagg acatactcaa
     7981 ggatgtatgt gtataagagt tgaagatgaa atgttcacag gagatacttt gtttgctgga
     8041 agtattggta gaactgattt atatagtgga gactttaacc aaatggaaaa atcacttaag
     8101 aaattatgta aatatgaaga tgctgttaga gtatatcctg gacatggacc aaccagtact
     8161 ctaggggttg aaaagaagac aaatccttac atgtaatttt atattagtat ttaaaaaagt
     8221 ataaaatatt taataactat attagtgagg gggttcttct actgttaaat agaaggagtt
     8281 aagttgatgt tgtatgtttt tttaaaagga catgattata agtatgaagt agcagaactt
     8341 ataaaattgt ttactagtga atttaagttt aaagactcta atgctaacaa taatatagag
     8401 tcaaagcatt taataaatag attgatttat gagaatggtg tattattctc aagtactgaa
     8461 tactatgaaa atggaaactt aaagtacgag tcaatacaga atatagaaaa tatgaatctt
     8521 gaatttttag atggatttga tagcagcaaa acgattgaag aatttcttaa tagcctagat
     8581 agtgaaagta agaggaactt taaaaagctg tgtaaagaaa atatcaaaaa aagcatgttt
     8641 gtagtgctta aacaagtatt taattcttat gtaccatggg gaattttgac tggtattaga
     8701 ccagtcaaaa ttgtccacaa tttaatggat aaaaagctag atgataatag cataagaacc
     8761 attctaaaag ataattattt tataatagat gagaaaatag atttagctct tgaaatagct
     8821 aaaagagagc gattatttat gtatcctata gataaaaata aaatttctct ttatgtaagt
     8881 attccatttt gtccaactag atgtgtatat tgttcatttc cttcaaattc attaaaacaa
     8941 tttggacatt taaaaagaga atatgtttat aaattgattg aagaaattaa ggggtttgcc
     9001 aaagttatta aggagactaa gaaagaagtt gaaactctat atattggtgg tggaactcca
     9061 actacacttg atgaggaaga attagatttg cttataaatt cattgttcaa tgaattagat
     9121 ttaagtaaaa ttaaagaatt tactgtagaa gcaggtagac ctgacactat tacagaacaa
     9181 aaactaagag tgttaaaaaa acacaatgtt agtagaatta gtataaatcc tcaaactatg
     9241 aatgatgata ccttggtaaa gattggaaga gagcatagag tatctgattt agtagattgc
     9301 tttaatatgg ctcgtagaat gggatttgat aatataaata tggatataat tttaggtctt
     9361 atagatgaag atttaaatat ggtaaagaat acattggagg agataaaaaa actttctcct
     9421 gaaagcctta cagttcatac attagccata aagagagcat ccaacttaaa tataaatatg
     9481 gataggtata aagaacatct aacacaatat gaagaaatgg taaaaatgat agatttatca
     9541 atgaaatatg caaaagatat gggattaaat ccatattata tgtatagaca aaaacatatg
     9601 ttgggaaatt tagaaaatat aggttatgct aaagaaggat atgagtgcat ttataatata
     9661 cagattatgg aagaaaaaca aagtaactat gcattaggtg caggtgctat atcgaaattt
     9721 gtttatgtag atgaagatag aattgaaaga gtagaaaatg taaaaaatgt tgaacaatac
     9781 attgaaagag tagatgaaat gataaaaagg aagaaagaag aggtatataa aaatgttgac
     9841 taaagctcct agaggaacta aagacataac tccaaaagag gcttacaaat ggcgttatgt
     9901 agaaaataaa tttagagaaa tatgtgcttt atatggatat gaagaaatgg taacacctat
     9961 atttgaacat acagaacttt tcaagagaag tgttggagat actacagata tagttcaaaa
    10021 agaaatgtat tcttttaaag ataaaggtga tagagaaatt accctaaagc cagaaggtac
    10081 agcaggtgta gtaagagcat ttatagaaaa taaattatat gcagatactc aaccaacaaa
    10141 attattttat gtaacacctt gttttagata tgaaagacca caagctggta gacaaagaca
    10201 atttcaccaa tttggaatag aagcacttgg aagtgatact ccttcaatgg atgctgaaat
    10261 aatcgcacta gctgttcaat tttttaatga agttggatta aatgatttgg tagtgagtat
    10321 aaattcagtt ggctgtccag tttgtagaaa agagtacaat gctttattaa aagagtatct
    10381 tgactcaaaa gctgatatat tgtgtgatac ttgcaatgag agaagagaaa aaaatccaat
    10441 gagggttata gactgtaaaa atcctacatg taaagaaaat atcaaagata taccatttat
    10501 tgcagaccat ttgtgtgatg attgtaaatc tcactttgat aaacttcaag aatacttaaa
    10561 ggaaatgaat ataaactttg taattgataa aacaatagtt agaggcttag attactatag
    10621 aaaaactgct tttgaaatta tatctaatga tataggagct caaagtactg tatgtggtgg
    10681 aggaagatat gatggtcttg ttgaacaatt aggaggacca aaaggtataa gtggaattgg
    10741 gtttggacta ggaattgaaa gacttttact tacacttgaa ggtaatggaa ttgaaataga
    10801 aaaccctcaa tctacggata tatttatagt aactataggt gaaaaagcaa atacaagaag
    10861 ctttaaattg ttaaaagact tgagacaaaa tcatataagt gcagataaag accatataga
    10921 gagaagtgtt aaagcacaat ttaaatattc agataaaata aattcaaaat ttacaatagt
    10981 aattggagat gatgagctta aaaatgacac tgcgacatta aaaaatatga aaacatcaga
    11041 gcaaactact gtaaaattaa gtacattagt tgaagaatta aagcaaaagc tgtaaaataa
    11101 gaaagtatga tgttttgtgt attagataaa tatatagcat taaaattaaa taaaatggag
    11161 gtagtatctt ggaaacttta aagggtttaa agagaactca ctactgtgga gagttgagag
    11221 aaaaaaacat aaatgaagaa gtcgtactta tgggatgggt acagaaaaaa agaaatttag
    11281 gtggtcttgt atttgttgat ttaagagata caagtggatt atgtcaaata gtttttgata
    11341 cagatgtaga taaagaagct tttgaaaaag ctgaaaaatt aggggctgaa tttgtaatag
    11401 ctgtaaaagg aaaagtatgc gaaagacaat ctaaaaaccc taatatgcca actggtgata
    11461 tagaaatatt tgcaactgaa cttagacttt taaacaagtc agaaactccg cctatttata
    11521 taaaagatga tgatgatgtt tctgaagcac taagattaaa atatagatac ttagatttaa
    11581 gaaaaccatc tatgcaaaga aatttaaaat taagacataa agtaatgaat attacaagaa
    11641 attacttatc aaacaataga ttttgcgaaa tagaaacacc atttttaata gctccaacac
    11701 cagaaggagc aagagattat ctagtaccaa gtagagttaa tccaggaaaa ttttacgcat
    11761 taccacaatc accacaatta tataaacaat tattgatggt aagtggtatg gatagatatt
    11821 tccaaatagt taaatgcttt agagatgaag atttaagagc agataggcaa ccagagttta
    11881 cacaaataga ctgtgaaatg tcttttgttg agcaagaaga tgttatgagt atgattgaag
    11941 gtttgcttga agcgatattt aaagaagttt tagatgtaga attggcactt ccacttccta
    12001 agatgactta tgcagaagct atgtctaaat atggttcaga taaaccagat actagatttg
    12061 gatatgagtt aacagatata tctgatgttg tttgtaactg tggatttaaa gtgtttgctg
    12121 atgctacaca accaggaaaa agtgttagag gtataaacgt aaaaggcaaa gctgacgact
    12181 ttactagaaa acaaatatca tcactagaag aacatgctaa gacatataga gcaaaaggtc
    12241 ttgcttggat gaaagtagga caagaaggag taacttctcc aatagcaaaa ttctttaatg
    12301 aagaagaaat gaacgctata cttacaagaa tgaatgctga agtaggagat ttacttttat
    12361 ttgtagctga taaaaactca atagtatttg atgctttagg acaagttaga cttgaagtag
    12421 caaataggct taatctattg gataaaaatg tatataactt actatgggta actgagttcc
    12481 cagtttttga agaagatgaa gaaacaggca cattctcagc aatgcatcac ccatttacat
    12541 cacctatgga tgaagattta gacaagttag aagaaggtga taagtcatct ttaagagcga
    12601 aagcttatga tattgtattg aatggttatg aaataggtgg aggaagtgtt agaatatcaa
    12661 attctgatgt tcaatcaaga atgtttaaag cacttggttt tacagaggaa agagctaatg
    12721 aaaagtttgg ttatttatta gaagcattta aatatggtac tcctcctcat gctggtctag
    12781 cttttggtct tgatagactt gttatgttac ttgcaggaac tgataatata agagaagtta
    12841 ttgcattccc taaaaatcaa aatgctgttt gccctatgac aaatgctcct acattggcag
    12901 aagatgagca attagaagaa ttaagtataa aagttgatat aaaagataat gaataatatg
    12961 tagttgcaag aaactaaaat gtaaaaagtc tggatataaa gtatatttat attcagactt
    13021 tttttattag aattatttta tattttttct ctatatattt tcataaatta tctttgtatt
    13081 ctcagccaat ataattaaaa tactatgttt aaaaatttta cataaacttg attgttttag
    13141 aaactacttc tgtaatgagt acacgatttt aattaaagaa tatataacaa atttgaaata
    13201 agtctaatat atgacttaat agattaaatt aaaataataa atttggacta tttatctatt
    13261 tataacttaa attatatcaa attgataaca aactagatat tatacttttt tgtagaatag
    13321 aattacaatt atataaagaa aaattctatt aatagtgaaa aaaaagtaga aaaaagtaga
    13381 aattattctt ttataaagaa agggagggat aatattgcaa gagagagaag aattagtttt
    13441 aaatccagtt ccaaaagagg aaagagttgg atggttagct ccattgttta atatgcttgg
    13501 ttctaacata gctatatctg aactaatggt tgggggaact ttaattttag gaatgactct
    13561 ttctaatatg attattacat caattatagg gaacttaata ttagttgcaa ttattatgat
    13621 acaaggttat ataggttaca aagaagggtt aaatacgtat gtattagcta agggggcatt
    13681 tggagagata ggaggaaagt atcttatatc tctattactt ggtataacta gttttggatg
    13741 gtttggtgta caagcagggg ttgcaggact atcagtacaa aagatattcc caagtgtaaa
    13801 cctaacatta gttacagtta tacttggatt atttatggtt gtttttgcac tgtatggctt
    13861 taaagctatg gctaaattta attacttagt tataccacca cttatgatat taatggtgtg
    13921 gggagttttt aaagcttttt ctacttatgg agttgaagct atttataatt atactcctca
    13981 aacaactatg agtatggttg aaggtcttaa tattgtagta ggtcttgtta tagttggtgc
    14041 aattatatca cctgaccagt taagatatac gagacgagta aaagacattt ggattatcag
    14101 ttttttaggt ctagggatta tttcattatt ccaacaagtt gcagctggtg taatgtctat
    14161 gggagcacct acttgggata taacagaagt attggctaat ttaggttttg gatgggtagc
    14221 gtttgttatt ttaatacttg catcttggtc tacaaacgta tcaaatgctt attctggtgg
    14281 attagcatta aaaactattt ttccaaatgt aaaaagaaat gttttgactt tagttgcagg
    14341 tctaataggt acaataatag cagctactgg tataatattt aaatttcagt catttttatc
    14401 tttcttggga atcgcagttc ctgcaatagc aggaattatg tggtgtgagt attattttat
    14461 acaaggaaga acttataagc atagagaagg aataaattgg attgcagtaa taagttggtt
    14521 aataggtttt gcagcgtcgt attatagttc aaaaataaat ttcttaatac ctccgataaa
    14581 tggaattgta gtgagtatgg ttatttatta tatattaatg aattgttttg gaatcaaaga
    14641 taaataaagg gggcaattac atgggtcgta aggtgaaaat aggtataatt caacaacaca
    14701 gtgtacttgg aaatgttaaa aaaaatatag aaaaagcagt agagatgata gatgacttag
    14761 gaaaacaagg ggcagatata atttgtttac cagaactatt tgctacagga tataatttag
    14821 agtctttagg tggagttaaa acattagaat taataagaga acataataag tacatagagg
    14881 aatcaatgtc agaagctgct aaaagaaata atgtatacct gatatctcca tatggaacct
    14941 tagaaaaagg gtctacacat gtttataatt cagcagttat atttgataga aaaggtaaga
    15001 ttatgggaga atactgtaaa aatcatctgt ggtcattaga agcagtatac tttaaaggag
    15061 gggaaaaagt agaggtatat gatgctgatt ttggtagatt tggtgtaatg atatgttatg
    15121 atgcaggttt tccagaagta tccagagaat tgacattaaa aggttctgaa ataatattta
    15181 taccatctgc atggagaata caagatgaag atatgtggga tttaaatgta tctcaaagag
    15241 ctttagagaa cactgtatat acagtaggtg taaatttagt aagtaatgac tctaatttaa
    15301 tactatttgg aaagagtaaa atatgcaatc ctagaggtac tgtaataact caattagata
    15361 catacaggga aaaatatgta ttatcagaga ttgatttaga tgaaataaaa aagtataggg
    15421 aagatatccc ctatttgaaa gacagaaaaa gtagctataa catataattc tatactataa
    15481 tacaagttca atataaaatg ataaaaagag gatgtatcaa tattaagatt aaatttatta
    15541 tactttgata tatcctcttt aatattatca actttgattt aaatattttt aaaatagttg
    15601 ataagaaagt ttttgaacaa caacacagct ttagattcat agttttcgct aaaccatgaa
    15661 agatttaaat atcttttaaa ttcaatatct ttgatatgta acaacttaat ctcagggtct
    15721 tgagataatc cccatgattt tccacatata aatccaactc cttgaagtga ttttataaga
    15781 gcataaattg tatatggtga atcagactca aatgctattt tgggtttgaa attagctgat
    15841 tcgcacagga tatcaattac ctctctataa ttctcaccct tcgttataac tataaagttt
    15901 tcatctgaaa cttcgcttag atatacttca tcctttaatg ataaaggatg gtttattgaa
    15961 actcctaata gtatttcttc gcatgttaaa gttatgctat tttctgagtt tagctttgta
    16021 aatgaagaag atatgtacaa atcaaaatca ctttttttat aagatgatgg caaagtatgt
    16081 gacacattaa aagtaatatt gggatatagt tttctgaaat tactcaaaag tgatggtaac
    16141 acattagccg ctgcaggaga taatatcttt acttctcctg aattttctaa agaagtgtct
    16201 tgtaactcct tttttgaact ttctactaaa tctaaaatgc tatctactct ttttagaaac
    16261 aatcttccat actcatttaa aacaatatat cttccttttc tgtcaaataa ctgaactcca
    16321 agttcttttt ctaaggaaga tatagttttg cttaaagctg gctgtgctat atttaacttg
    16381 tgtgatgctt ttgttatgtg ctccattcta gcaacagttt gaaaatactt aagatgcaat
    16441 aaatccatag tataaaatac acctctttta atatgacttg agttatatca attttataat
    16501 caactccaca ttatacatta taatataaag tttatataat aaactacaat atattaaaaa
    16561 gttatttaaa ttgttattta tattttgtac ataatctaat taaaaaaagt aactttaaat
    16621 gattaactaa ggaaatattg ttacaaactt tagttatata aaaaattcat ttgatgagca
    16681 aagtattaat gtcaagtaag aataaaagct tattagataa gaaaaataat attttataat
    16741 aatgatgtta cttaaattat tatgttatac tattttaaat aggaaacatt tagatactat
    16801 aattttaggg ggagtcaaga tgaaattaac taaatcaata tcatcaatgc tgtgcttatc
    16861 tctagtttta agcttatcac cagtagcata tgcaaatgaa ataagtagaa caacagttga
    16921 gaatacttta gtaggtcagg atagatatca aactgctgtt gaagtaagta aaaaaggatg
    16981 gagtagtgca aatgaggctg tagttgtaaa tagtgaatct attgtagatg cattagctgt
    17041 aacaccattt gctaaattaa aaaatgctcc tatattattg acagaaaaaa ataatctaaa
    17101 ttcacaaact gagaaagaat taaaaagatt aggagttaaa aaagtttata taattggtct
    17161 atctagttct gtatcaaatg atgtgcaaaa acagttagaa tctaataatt taactactga
    17221 tagagttggt ggtagtgata gataccaaac agctttgagt atagctaaaa aaatagaagg
    17281 attaaaagat atatcagaaa tagctgtagt aaatgggtac acaggtcttg cagatgcagt
    17341 tagtatagct tctgtagctg ctactaatgg aatggttata cttccagtat ctgatgcatc
    17401 tggtatatct agctttaaag attttataga atctaagaat ataaataaat catacataat
    17461 aggctctact aatgctgtat cagataaaat aaaacaaagt ttacctaatt cagaaagaat
    17521 aggtggagca gatagaaatg aaactaatgg taaagtaata gaaaagtttt atacatctaa
    17581 taaattaaac aatgtatttg tggctaagga tggaatgaaa aatcaaaatc aacttataga
    17641 cgctctagca gtaggagtac tagcatcaaa agaaaattct cctgtactaa tcgtaggaga
    17701 acaattaagt gcaacacaaa agagtgtatt ttcgaataaa aatgctacct ctataactca
    17761 agttggtagt ggaggaaatg aaaatgcatt taaagaatta aaagatttac aggcaaatag
    17821 taacaataag agagtaatgt atgtaactaa tacagataag gtaaatataa gaaatgatgc
    17881 tacaatagaa gcttctgtta taggatatct aaataatggt gatgaagtag aagttttaga
    17941 tgtgttaaaa actggatggg taaaaataaa atacaatgaa ggtattggat atgttagtgg
    18001 gtcttatttg acaaataata agcctgataa tagtaatgaa aatataaaaa taaagtatgt
    18061 aaaagaaaaa gatggtctaa atgttaggaa aggaccaagt acagaagatg aaaaaatggg
    18121 tcaactttca tatggaagca aagtagaaac agtagaaatg tttgctactg gatgggtaaa
    18181 aataaagtat aatggtggat atggttatgt aagtaatgat tacttatctg atacaccagt
    18241 tatttaatta taagtaacaa taaaaaaact gctttaattt gtaaagcagt ttttttataa
    18301 aaatttaagt ctaataacta tttctatttt gataccttaa tcctgggatt tattattatg
    18361 gatattatca acatacaaac actccaaatc cagtgtctaa ttccaaactt agatattaat
    18421 aaattcagtc ctgctatggt aaataagcag tatttaatta caattaaaag tgtatttata
    18481 gaaagcccct tcatagaaag tatcctaaat ccaataatag acattctaag tcctataaat
    18541 gccaataata tacctgcaat atcctgaatt aagtaatagt attcaaattt catattcaat
    18601 caactcttct ctgagcagta tcaaatattt ctaactcttc aatttcatca tctgtgtatg
    18661 taagcatagt aaaaccagtt acaccataaa taaatgaaat tataggtgta atccaactta
    18721 agaaacaata tggaataaat tctagtgggc ttacaccaag agtctgagca cagaaaacag
    18781 catttgaatt ccatggtatt aatggaccac caagtgtagc tgtatcttct ataattctag
    18841 atagattttg tggttttaat ttaaattctt tgtatattgg agacattaga gttccgacaa
    18901 atacagaagt gaacatcatt gatgaactaa atgcatttgc aaagtagctt actataaaag
    18961 tagacccaac caatttagtt cttgattttc ctattttagt tataagaggt tgaagcagta
    19021 cttctaagaa tccagttttt tggagaatac cagcaataac gaaaactaca aatactagta
    19081 aaacggtttc agccatactc attattccac cacgatttaa tagtttgtcg gcatagacaa
    19141 atccagtatc tatagtaaat cctgatagca tgtaatttaa aacatcacct attttttctc
    19201 cttcttgaaa tactgctata ataagtccca tgatagctga acttagtata gatataatgg
    19261 gtggcttttg tagcaataaa agtaataata aaaatattat tggaatcatt gctactaaac
    19321 caatatgaaa gtttgaattt aatacatcct tgatttgatt tatttgaaca tagtctatag
    19381 tattatttga gtacttaaat ccaattactg tatacagaac tatacatatt acgtatgcag
    19441 gtcctgtggt ataaaacata tgcttcatat gtgtaattac atctgtctta caaactgctg
    19501 cagctaagtt tgtagaatct gatagaggtg acatcttatc tccaaagaaa gcaccagaaa
    19561 tgactgcacc agctgttaaa ccaagaggaa tgcccatact agtaccaata ctcatcattg
    19621 ctataccaga agttccaaga gtcccccaag atgtaccagt agcaacagaa gttaatgaac
    19681 atacgattaa tgttgtaagc agaaaatatt taggggttat tattgttagt ccactatata
    19741 ctactgttgg tattgtacca gcagctatcc aagctgctat tagtacacca acagaaagta
    19801 taattaagtt tgattgaaag gcatctttac caacttcaaa tccaaaagct tctatttcat
    19861 cattagtata accaagtttc atggctgcag gaactactat aagccatgaa agtaaaaaca
    19921 ttgtagttat agaagcatta aatacaacta taccaactga tgttagggct attattgcaa
    19981 gcattactaa caaggaatat ctaaatgaag gtatttttct ttctgtattc attttcatag
    20041 tgcaaattct ccttaaatat ttttagttaa ttgcattttt tattcttata agagcttctt
    20101 caagcatact tcttggacaa gctaagttta ttctttgata cccacttcct ccaatgccaa
    20161 agctgtttcc ttgatttaga gcaactttac ctttttgtac aagtatactt tctagttcct
    20221 catcacttaa tccaagtgca ctaaagtcta cccataataa ataagtacct tctggttttc
    20281 taactttaag ttttggcata ttttcattta tatattttat agcaaaatct atattagatt
    20341 ctaaatattc taaaaatgat tctagccaag attcaccatt attatatgaa gcttcagttg
    20401 caactaagct aaagcaatta tttctcttta tatcaattct ggtaaaggca tcatctaaca
    20461 atttatagtc tttttcatca ggtagtatta catatgatga ttgaagacca gctatattaa
    20521 aagttttagt tggagccata caagtaattg tatttttttc aaattcttta gaaatagatg
    20581 ccataggaat atgcttatgt ttttttaata taatgtcact atgtatttca tcagatatga
    20641 tttttacatt atgcttaaga caaatatctc ctagtttttt taattcatct tttgtccata
    20701 ctctaccaac tggattatga ggattacaaa gtatgaataa ttttacatct ttaatcttgt
    20761 tttcaatgtc ttcataatcc attatgtagt ttccattttc tagtttttgt aatgggttta
    20821 taattagttc tctattgtta ttttttacaa cactattaaa agggctgtaa actggttctt
    20881 gtatcattat tttatcattt gcttttgtga gttcatttat taaaagactt atagctggga
    20941 taacacctgg actataaatt aaccactcac tttttatttt ccagttatgt ctcctatata
    21001 gccagtttac tatagattca ttgtaagaat ctggtcttgt tgtgtagcca taaatctctt
    21061 gttctagtct attttttaag gaatcaatta tacaaggtgc agccttaaaa tccatgtcag
    21121 ccacccacat aggaagtaaa tcattagttc catacttttt ttccatttct gaccatttag
    21181 atgaaaaatt attgcttcta tcaacaatct cattaaaatt ataattcatt gtaaatacct
    21241 ccagtaattt taattttatg ttaataatat taagcaaaca acgtgccaat attgataata
    21301 aaaaatattt attcacaagt taatatttaa tattaagtaa tttcagagca tagataagat
    21361 aaaaatatta attatactta aaactttgtg gaaaaggtta tttctaataa atttttaaat
    21421 atatgaaaat gtaattaaat aggttaaata ggtttaactg tttaacttgt tagacctatt
    21481 tatatttttt agaaattcta ctccttttga tgttataatt gaacctccac gaccaacaag
    21541 tatgtctaca agcccaaagt cttgtagttc taaaagcata tttctaatct gttgttctgt
    21601 caaaaatcta ttttcctcta aagctatttt acataagctt tttctaccta ttctttcttt
    21661 taaatcataa gcctttttta aattatccaa tataaatatg tattcatcta gtgacctttt
    21721 aaaattatag tcgtatttca tgatgttttt gtctttgcca atgttgaatt gatacttttt
    21781 tatattatca cttactttat tacaaacggt tctactataa tttgaatcta tagtatccaa
    21841 tatatattca ggtaaatcac atatttctat aatatctttt tctaagtaac agaagtattc
    21901 tcctaagttt cttaattctc ttacatttcc ttcccatcta tacattttaa agatttcttt
    21961 tacttcctca cttagaataa aattacatgg aatatcatat tttaatgaac caaaaatttc
    22021 aaatatatca gcaggtcttt ccctaagtgg atgtatttta aggggaagaa cattcaacct
    22081 aaaatataaa tctcttctaa acatattttt agatacaagt tcttttaaat ttctattagt
    22141 agctgcaatt attcttacat caatgtcaat gacattatta gaaccaatac gtcgtacttg
    22201 tttttcttgt atgactctaa gtaatttaga ttgagaattt aaatccattt ctccaatttc
    22261 gtctaaaaat atagttccat tgttggctaa ttcaaataaa ccaattttcc cacctttttt
    22321 agcaccagta aaagcccctt catcatagcc aaataattca ctttgcagta gattttcttg
    22381 aaaagtacta caatttactg cgacaaaagg accatctttt ctcctagaag cactatgaat
    22441 ggattgagca aataattctt taccagtacc actttcacca attataagta tagatgaatt
    22501 tgattgagcc attttattag caattttttt tgtattaata gtttgtatac tagaacctaa
    22561 tatatcatca aatgtgtatt tactgatatt accagagttt actagttgtg ctcttaattt
    22621 agcttgcttt ttttctaatt gagaaaactt tgtcatcttt agtataaatc catcaaatac
    22681 atttaattgt atatgtttaa tctctaaatt tatatcaata tgatttattt taatcaattt
    22741 ctcaaaaaat ggagttttat tggtcaatat gctttgaaaa ttgatgtctt ttatgcaatc
    22801 accaacaaat gagtcaatca tttcatttgc atttatagac aaaatttttc tagccatatg
    22861 attatagaat tgaattattc catcgttatt tgtgcatata attccatcat caattataga
    22921 tagtaacaaa tcaaattgtc tttcaaactt atttgcatct aacaataact cacctgtact
    22981 ataacttgta ggaattattt tttctacgta tttttttatt aggtcatctt ttattagatg
    23041 ttttaatttg gtttttgtag caatttcaac aatacaactt aaatctatta ttctgtattt
    23101 taaatctata acttcacaat ttttaaactt aggcaagatt ttttcgccag gagttaggat
    23161 tacagcatct gtgggagtaa actcaatctc tggatagcag ggcaggatat ttatattgtc
    23221 tattcctaat ctatgtatta aagaaattgt ttctattgcc atatctttac ttaagttata
    23281 tacataaata attttatctt tttctaattt ggatatcata tcaatactgt tcttttcaaa
    23341 tgtcaacttg ggaacaatga tttgagcatc atagtgagat aattttacta tttcatcata
    23401 ttttaaataa gcagatagga gtataagttt ttctttaaaa aactttaaat ttccctcttc
    23461 aaatgagtat aaatttatct ttagattatc acctaagaat tgtcttattt gattttcata
    23521 aatttctcca gcgtcttttt ttaaacttac aatagctaat tccatttgtt tcattttaac
    23581 ctctcataca aatttgactt attttagatt acataataat taaattagat taaataatat
    23641 tatttaatta ttattataaa tttattatag actacacatt gaagtaaaag aagtagctaa
    23701 aattattcct tttatttttg ttttttgtat ctatttggta taatagtatt agagcgtata
    23761 ataatttagg caaaattttt taaggaagac tagaggagaa tataaaatga aaaaatgtct
    23821 ttactgtaat agggatataa agtcaccaaa taataaatta gctataaaat atgatgacaa
    23881 tatgaatata tatccatgtc gtgcagaatg taaggaaaaa attgaagaat atatagcaaa
    23941 aaaacctaca tataagttta taaatatatt agaggttctt ttaggtgtgg gcggaatatt
    24001 ctgctttttg agtaaatgga ctattgcagc ccaagttgta cttggaattg atgcactggt
    24061 tatattttta ttcccattgg caggatttaa gatgaatgga aagttaagta tggaacaaac
    24121 taagaatcaa agtaggtcta ttgcaatatc aattttggca tttatagtaa taataactgt
    24181 attatatttt aaacaagttg gtaagtagag cataattgta taagacatca ttagagtatg
    24241 aggtgagttt atggaagaaa gaaaattagc accaaacaat gagagggaag ctctcataaa
    24301 aagattaaaa agaatagaag gtcaagttaa gggtatacaa aagatggtgg aacaagaacg
    24361 ttattgtgtt gatatattgg ttcaaatatc tgctataagg tctgctataa atagagtagg
    24421 cacaattatc ttagagaatc atataaaagg atgtgtagca gaaagcatta aacatgatga
    24481 tgctgataaa actgaagaga tgataaaaga gcttatggat actataaata aatttacaaa
    24541 ataaggaggc ggttagttat gaatcaacaa ggatttatta tggaaattgt tgatgataga
    24601 actgcaaagt taaaaatgca aagacattca gcttgtgctg cttgtggaaa atgtgctact
    24661 acaaattcag cagagtcaaa agaaatatta gtagaagtag acaacacaat tggtgcaaaa
    24721 gttggtgacc atgtagaagt gagtatggat aatatgaatg tattaaaagc agctgtaatg
    24781 gcttatataa tacctcttat agcacttctt gtaggaacaa tagggactta ttatatattg
    24841 ggggcaattg gaatgacaac tggagttgaa gccataagtg gattagttgg aatttgtctt
    24901 acaataatct gttatttata cttaaagaag aatgacaaaa aatttagaga cagtagagaa
    24961 tttataccag taataacaag aatattaatt gacctttaag ataattataa ttaaaaaata
    25021 gcaatgtact ttttaaataa atactcaata cgtatttgta gagtttaagt acattgctat
    25081 ttttatattt ttattttgat tttctgtaag gaataaagtt ttttctgatt tttaaaagat
    25141 tcaatgttct tacaaaagaa gttacaacta atacaccaag tgctattgca gcacttagaa
    25201 atagagcatc tgtacctttt gacatagcca aagaataatt tttctgaatc aaagacaaca
    25261 ttgtattata tagcccaatt cctggtatta gaggaagaat acctggaatt acgaacacaa
    25321 tagctggttg ctttagtttt cttgctaaag tctcactgca cgctgagacc aaagcagcag
    25381 cgataaaacc tgcaaaaact gcatttgtag taaaattaaa taaataaaca tatacaatcc
    25441 aaccaattcc gccagttact ccagctggaa gaagtagata tagtggagca ttaaagaaaa
    25501 cagcaaatcc agcagttgca acacctgaaa ataaaaaatg caaaatcata gacatatcag
    25561 tcattagaaa ggacctccta ttctatacca tgtatcaagg acaaaaccaa ctccagaagc
    25621 aattgcaata ataatcatag cagcatcaaa agcacgagaa attcctgata ttaaattccc
    25681 tgatattaaa tctctaatag cttttataaa agcaactcct gggagtagtg gcattatagc
    25741 gccgactata agcattttag ggtcatctaa aatgcctatt tcaactagta gaactcctga
    25801 aagtgcaatt aaaaaagaag atactaaaca ggaaaatgca gatattgcac ttatcttcat
    25861 cgttttatca taagtaattg cagcaaagat tgctattata aacgtaagta taaaatttaa
    25921 tacataattc cctccaagca aacaagcaaa acaagcagat gcaattcctg tacaagagta
    25981 aaataaccat aaaggataag gttttacatc ttggatttca taaagacgtg caatggcatt
    26041 atcaatatca tactctttgt cactaacaaa ttctcgagaa aaactgttta ataaagaaat
    26101 cttatttaag tttatacctc ttgattgaat cgttttcata aacgaaaagc catcaaattt
    26161 ctcatctgaa ataattatta ctgtaggagt tgtaaaaata tttacatgat aaaaccctct
    26221 tgacctgcat attctaagga cactgtcttc aaccctagat gtttcagctc cattttttat
    26281 catcagttcc cctacaaata gagctagttt tagaatattt ttcttatatt caagttcctc
    26341 attttgattc ataaatacac ctccaaatta aaaaacgtaa tattgaaatt atatcaaatt
    26401 tttaagtaat attccttaat atctatataa atatattatc ttttttttgt ttcaaaatag
    26461 tcacatttaa attatatcca a
//
LOCUS       DAGSRM010000045        26439 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.045, whole genome shotgun sequence.
ACCESSION   DAGSRM010000045 DAGSRM010000000
VERSION     DAGSRM010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26439
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.045"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            139..1014
                     /locus_tag="MJE33_002095"
     CDS             139..1014
                     /locus_tag="MJE33_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HBY9648029.1"
                     /translation="MNVYEKGNGVILEGVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     KGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYTEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKYIGEYEGNEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDEVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            1032..2522
                     /gene="cls"
                     /locus_tag="MJE33_002096"
     CDS             1032..2522
                     /gene="cls"
                     /locus_tag="MJE33_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBY9648030.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNVGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTDDFFEDLSYCEELNLEVFKNRNIIQKIKESVARLFSPIL"
     gene            2715..2999
                     /locus_tag="MJE33_002097"
     CDS             2715..2999
                     /locus_tag="MJE33_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBY9648031.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTVGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            3032..4660
                     /gene="groL"
                     /locus_tag="MJE33_002098"
     CDS             3032..4660
                     /gene="groL"
                     /locus_tag="MJE33_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBY9648032.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVTVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKESTTIVDGS
                     GDKKAIEDRVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVVKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTEAAVADLPEKEDAGMPGMGGGMPGMM"
     gene            4922..5662
                     /gene="larB"
                     /locus_tag="MJE33_002099"
     CDS             4922..5662
                     /gene="larB"
                     /locus_tag="MJE33_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HBY9648033.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDLGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYNHAEYEEASK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDSARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLASMINKL"
     gene            5746..6480
                     /gene="larC"
                     /locus_tag="MJE33_002100"
     CDS             5746..6480
                     /gene="larC"
                     /locus_tag="MJE33_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HBY9648034.1"
                     /translation="MSEKILYFDIISGISGDMTLASLLNLGVPKEIFLEEIHKLRMSD
                     EFNINISSKTENGIVGTKVEVITKEKHTHRNLVDIFEVIDKSTLNENVKSKAKKIFMV
                     IGEAEAKVHGTTIDKIHFHEVGAIDSIVDIVGASILVDLLCVDKVCASTVSVGSGFVK
                     CEHGIIPIPAPATIEILKGVPIKLNNVNGECTTPTGAAIIKVLCDEFVDELEFSPKQI
                     GYGIGHKKFEVPNILRTVLGEKKKKN"
     gene            6510..6929
                     /locus_tag="MJE33_002101"
     CDS             6510..6929
                     /locus_tag="MJE33_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HBY9648035.1"
                     /translation="MSSEIYSYLYEKILDEKALDIYTESIYMKKNRPATKLSILCNES
                     DLEKFIKILLLETSTFGVRYNAYNREILERKFVKLDTKYGVVTVKLGYYKGKLVKATP
                     EYNECKTIAKNEDIPIKKVFADINFRIKEEFSENLLT"
     regulatory      6989..7090
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            7311..8846
                     /gene="guaA"
                     /locus_tag="MJE33_002102"
     CDS             7311..8846
                     /gene="guaA"
                     /locus_tag="MJE33_002102"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBY9648036.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGVPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTAITYGKSSLFEGITTNSVWMSHTDLIEKVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            9385..10815
                     /locus_tag="MJE33_002103"
     CDS             9385..10815
                     /locus_tag="MJE33_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBY9648037.1"
                     /translation="MILNQIFIEAGGIMRNKIIIGITFSLVLLMSGCASKQTAEINVL
                     ATTDLHGVIPYELTSYVKEERKKDKNLTLVDAGDFFDSGEVFGSSMDSYFSARYKDID
                     NNTERYIETPISKDMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQKINILSANTY
                     KKNGDSYTKPYIIKNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQE
                     GYNGELYMNDLVEDAKKWVSIMEKEKPDIIVAVAHTGEKPKKPRNPGNRIQDLAQQVD
                     GIDAIVAGHNHVQIEQHDYKNKSGENVIVTEPGKHGECISKINFKLEKNNDKWNIVDK
                     SSKLIKFEKNQESDKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDI
                     YKTIGYKWRNISSPKGDDMVQIVVMNNNKPVSYVYGEAEVMPINIKCDESEYKDNVVT
                     IYPNKNDKFKIEKGKQEYSINLKHIQ"
     gene            complement(10963..11313)
                     /locus_tag="MJE33_002104"
                     /pseudo
     CDS             complement(10963..11313)
                     /locus_tag="MJE33_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            11308..11829
                     /locus_tag="MJE33_002105"
     CDS             11308..11829
                     /locus_tag="MJE33_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBY9648038.1"
                     /translation="MFSIGGGYAIIPLIQEKVVNQTGWISEKMFTDIITISQMTPGPL
                     AVNTSTFVGLQIGGFWGAFAATLGCVLCGVVIALFLYNFFQKHQNSTYIFEILNGLKS
                     ASLGLIISAAATIIILALFGTNKVYFNSIFEMLNWTALIIFFVTLLIVRKWKINPIVI
                     IIISGILGIFFYL"
     gene            complement(11958..12752)
                     /locus_tag="MJE33_002106"
     CDS             complement(11958..12752)
                     /locus_tag="MJE33_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBY9648039.1"
                     /translation="MIFCNSFITRKSSMNIVVTHTPIVTTLEMQKAYEPLAKHNVNIF
                     AFDFSGTGKSDGYEKDFSRNSIVKDLDSVITYIEKNYSSNIHLYGNTGIGGMFAQYYA
                     CSDNKLKSFAQFACVDYKNTTGVGYTYLTVKLLNFFFKFLPNFHITVKPPKYKGYNEV
                     YDNEFYKIFEKKNPHIWKNSTKIINTMLECFIAEDSKIKDGVNIPTLVFKTLHDRYFK
                     TQYFDRYYNSLKCKKKLVEINNVHNSYYIDSEKFCQEAYDWFVENQ"
     gene            complement(12976..16404)
                     /gene="uvrA"
                     /locus_tag="MJE33_002107"
     CDS             complement(12976..16404)
                     /gene="uvrA"
                     /locus_tag="MJE33_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBY9648040.1"
                     /translation="MKINNYKNQSIITNPKKFENKYQDLPKTPIELLKVVQSLVIHGD
                     QGKLYGISFNKQQSDEELLRTIPQMLKRIFEINSNPLTIPRNPKQRLVGMCRDYSLLL
                     VSLLRYRGFEARMRAGFANYFESELTYEDHWLVEYYDTLTKRWIRIDAQIDDIQKNYF
                     QINFDTHDVGKTDGFLTGSEAWIRCRQGHAHPDDFGYNKNWKGWHSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSSGNIKIEDLNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELGNPLELEKKFKFTKSINKSIKNSLCHNKSSIYLKGGRQN
                     NLKDVEVTIPKNQITVITGVSGSGKSSLAFDTIYEEGKRRYFENLSNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGSQNPRSTVGTLTSIWDYLRMLFVSIGKSYCPYCKIPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTISIL
                     DGATYYFGKLRGKKPNGNWMVGELYAIAKDKNIDLDIPWNELPRDFIDAILYGTDDKI
                     YEFSFESKGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELPNQLQKNELSLVNDILTELKIRVSYLLK
                     VGLSYISTDRTSPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDVGPSAGTKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKTGSQNKTIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSIPDKNYTEFTGFDAIDDFILMDQTPIGKSSRSTPATYINIF
                     DEIRSLFAETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYMADQWVTCSEC
                     QGKRYQKEILSIQYKGKTIADILDMEVAEAKTFFSDCSDIYRKLSLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEVIRASDWIIDIGPDSGINGGEIVASGTPDEIKINPNSITGHFI"
     gene            16723..18957
                     /locus_tag="MJE33_002108"
     CDS             16723..18957
                     /locus_tag="MJE33_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBY9648041.1"
                     /translation="MKLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISNKQSK
                     NYLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDVGI
                     IDLEGKAEFLSGKKFDLKNTASYKKGIEGKEYSEVIESMGFVLYSVPIFNDNHEVSSI
                     LIGVSNKESMNEILNIDNFNGEGTIEIINSKGKPLFIGKNTELIKDLSHKYKDSTDEP
                     WAQKMLNDFKNDKSGNITITSSKGVQCLLTYHPITRGLNDWHFLLIVPEDAVLGELNK
                     LNSFTITMTFIITCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSMLVKP
                     LISESPDSTYMMIAMNIKDFKLINDCFSSEKGNMTLKHLYNILYKNIDENEEFVCRHD
                     ADLFYLLIKNRPVLEALELLYKIEADTNYFNKNRENPYFLRLSVGIYTIENHNEDLIT
                     IQDHANTARKSFNKTHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKI
                     DIKSGKISGAESLVRWKNSEKGIIYPSDFIPLFEKSGFICKLDLYILEETCKLISKWI
                     KEGKEVLRISVNVSRQHLNDKLFLEKYKHICNKYNVPTCLIDLELTESIFLENPEAID
                     IIEDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSI
                     IELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFENFAYENNKIK
                     NIHY"
     gene            19213..21243
                     /locus_tag="MJE33_002109"
     CDS             19213..21243
                     /locus_tag="MJE33_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY9648042.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKSVIQKMNKGVLIIPDDFQVVCINNNEYVFSLTERVSIMKIISMFKIDNLNL
                     EKLSKEFQVSRSSIKNDLNVLSSELERNQITITYDKSFKISGEEDIIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGIYLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESASLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQKNRVLSQIKNENETILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLVNDYQINIVESIPSYKLQDYKEWESIDFIITTSK
                     IVIDLPKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAQKRVLS
                     IICQELGYSDERILFRQLNLSDLIGIDTIRIIDGDMKWEDAVKFSSNILANCDFVSAQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFGDKKVKIIF
                     CLASRDKKEQIPAIINLTKMVYKTNFIAMLESAKTKEEAEVLIHKYEKEVTV"
     gene            21240..21692
                     /locus_tag="MJE33_002110"
     CDS             21240..21692
                     /locus_tag="MJE33_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY9648043.1"
                     /translation="MNKLVNVKCSFKIHVNSKEEAIISLVDVVSKEGYLIDKNQFLKD
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDTPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEEED"
     gene            21694..22749
                     /locus_tag="MJE33_002111"
     CDS             21694..22749
                     /locus_tag="MJE33_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HBY9648044.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLIGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMAGSI
                     IGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAVLMKLVKKDVKEDE
                     EIEEDLDESIELIFEEL"
     gene            22816..23124
                     /locus_tag="MJE33_002112"
     CDS             22816..23124
                     /locus_tag="MJE33_002112"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBY9648045.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKDIDEATCVILTNDVEIRNMERFKGKKVVRMSVSEIIKKSDALIKKIKDTFQ
                     "
     gene            23228..24493
                     /locus_tag="MJE33_002113"
     CDS             23228..24493
                     /locus_tag="MJE33_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HBY9648046.1"
                     /translation="MKKLPIKTVVQRLLNLQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKVADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLPVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKVIPLDVVLE
                     RTVELIEYCENERKRLNLPDIGYEVGTEETNGGLTSTETYETFITRLKVELDNRGLPM
                     PTFIVGQTGTLTRKTEQVGIFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SQIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVEKTSNIRHVLLVNAIECGRWRK
                     WVVGEQKNLTTEEIFKDEVLSNEILDIAGHYTFNNDDVKKEIEVLYDNLSKNNIDGQR
                     FVVDHIKRPLRDYAECYNLKGVTTRILNK"
     gene            24581..25297
                     /locus_tag="MJE33_002114"
     CDS             24581..25297
                     /locus_tag="MJE33_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBY9648047.1"
                     /translation="MINLIFDVDDTLYNQLTPFYTAYNKVFSSIKDISIEDLYMSSRK
                     YSDEVFHMTENGEMPIKEMHIYRIMKAFEELGNSITEKDAQSFQDEYIYQQSQITLIP
                     EVERILNFSKERNINLGIITNGPSNHQRMKLKQLNIENWVDKSNIFISSEVGFSKPDT
                     NIFRVAENVMNLDRENTYYVGDSYRNDVLGAKKAGWKSIWLNHRGYEVEELFYKPDFV
                     ILEHKDLISLFIKICSYKNM"
     gene            complement(25492..25785)
                     /locus_tag="MJE33_002115"
     CDS             complement(25492..25785)
                     /locus_tag="MJE33_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HBY9648048.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWVKPYDKPIQQLTCSYKESQNVID"
     gene            complement(25791..26006)
                     /locus_tag="MJE33_002116"
     CDS             complement(25791..26006)
                     /locus_tag="MJE33_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648049.1"
                     /translation="MRILSETEKISLAAIIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAIQQFIVENEILISEEV"
BASE COUNT        10007 a         2953 c         4571 g         8908 t
ORIGIN      
        1 attattttac ataataataa ccttataaat tttgaaatta cttatctttg tgttaaaata
       61 agtaaaatgc acatattaga gctaagtttt taagtaaata aacaaagtga aaatataaat
      121 attaaggaga tttggtatat gaatgtttat gaaaaaggta atggagttat actagaagga
      181 gtaacagatt ttgacccagt acatatattt gagtgtggtc aatgttttag atggcacaaa
      241 caagaagatg gctcatacac tggagttgca aaggggagaa tattaaatgt caaaaaagag
      301 aatgataaaa tatatttaaa taatactaat ttaaaggagt ttaacagcat ttggtataat
      361 tactttgatt taggaactga ttatacagaa ataaagaata agctaaaaaa tatggatgaa
      421 tatttaaata aggctactga atttggatgg ggaataagaa tattaagaca agatggatgg
      481 gaaatgctta tatcatttat aatttcctct aataatagga ttcctatgat acaaagagct
      541 atagaaaatt tatcaagaaa gtttggtaag tatataggtg aatatgaagg caatgaatac
      601 tatgcattcc caacaccaga agaactaaat aaggcctcac aagaggaaat aagagcatgt
      661 caaacaggtt ttagagataa gtacataaaa agtactacac aggcagttat agagaataat
      721 gatgaggtat ctgaatatac aaatttaagt acagaggatt gtagaaaaga gcttttaaaa
      781 tttaatggag taggaccaaa agtatgtgat tgtattgcat tatttggtat gcaaaaatat
      841 gattcttttc cagttgatgt ttgggttaag agagttatgc aagaatttta tatagatgaa
      901 gacatgagtt tacctaaaat gagaacctat ggaatcgata aatttaaaga aatgtctgga
      961 tttgctcaac agtacttatt ttactatgca agagaactcg gtataggaaa ataaattaag
     1021 ggggagaaat tatgggagta ataggaacaa tatttctgtt ctatttaatt atatcatatc
     1081 tagctggagc cataatttct gtgattatat tacttgaaaa tagagaccct gcaaaaacaa
     1141 tgtcttggct attgatgttt attatatttc caggtgtagg tttaatgata tatgctattt
     1201 caggtagaaa tattagaaaa agaaaattat ttaagactca aaaactagct aataatataa
     1261 aagaaaagaa actatttgat acattagaaa agataacaga aatagttgaa ttagaaaaag
     1321 aatctataaa acaaaataag cttttaagag atgaagagga tggtagttat agaaaaaggg
     1381 taataaatat gttgttaaag actggtatgt ttccctttac taaaaataat aaagtggatg
     1441 tatttgtaga tggaaatgaa aagtttaaaa gacttataga agatataaga gaagctaaag
     1501 accatattca tttagagtac tttatcatca aagatagtga aataggtagg gtgttaaaag
     1561 aagaacttat aaaaaaggct aaagaaggca ttaagataag aattttatat gatgatgtag
     1621 gatgctggag attttggttt aatagaaaat ttttccgaga aatgagggaa gtaggtatag
     1681 aaattgcagc atttttacct acaaaatttc ctataatagg agggaaatta aactatagaa
     1741 atcacagaaa aattgttgtt attgatggaa taattggata tacaggtgga ataaatatag
     1801 gagatgaata tttaggcaaa aatgataagt ttggatattg gagagatact catattagga
     1861 ttaagggtat ttctgtatac atgcttcaaa tgacatttct gattgattgg tattatacga
     1921 caaaagaagt attagtgact aaaaattatt tcccaagtgt agggaatgtt ggtgaaagta
     1981 tgatacaagt tgttgcgagt ggtccagaca gtgactggga agatattcat tatgcttatt
     2041 tttctgcaat atgtcaggct agaaaaaatg tttatataga aactccatat tttattccag
     2101 atgaaagtct actaaaagct ataaagagtg ctgctcttag tggagttgat gtgagaataa
     2161 tatttcctaa aattgcagac cacaagatag taaatatagc atcttattca tattttgaag
     2221 aaatattaag agcaggtggt aaagtttatt tatataataa aggatttata cattctaaag
     2281 tcgtaataat agatgataaa attgcttctg caggtacagc taatatggat ttaagaagtt
     2341 ttatgcttaa ctttgaggta aatgcattta tctatgatga agaagttatt agggtaatga
     2401 cagatgattt ctttgaggat ttaagttact gtgaagaact taatttggag gtatttaaga
     2461 atagaaatat catacaaaaa atcaaagaat ctgtagcaag attattttca ccaatattat
     2521 aaaaaattat taaaaaaggt attgaaaatt atgtgtgatt ggtatattat aataattgtt
     2581 agcactctat tagatagagt gataataaat aaaaagtata tatataaatc tacatatata
     2641 agtagtatta atttaaaaat aactaaaaac ataggcgaac tagtctatac ttaatatttt
     2701 aggaggcgtg aataatgaaa ataagaccat tagctgacag agtagtaatt aaaaaagtag
     2761 aagcagaaga aaaaactgca agtggaatag ttttaccagg agcagctaaa gagcaacctc
     2821 aaatagctga agttgtagaa gttggaccag gtggaatagt tgaagggaaa gaaataaaaa
     2881 tggaattgac agtgggagat aaggttatat tccaaaaata ttctggaaca gaagttaaga
     2941 tagaaggaca agaatacaca atactaagac agagtgatgt attagctgtt attgaataaa
     3001 tatagaataa atttattagg aggggtttaa aatggctaaa gaaattaaat tttcagagga
     3061 aacaagaaga gctttagaag ctggtgtaaa taaattagca gatacagtaa aagtaacatt
     3121 aggacctaaa ggaagaaatg ttatattaga taaaaaattt ggttctccac ttataactaa
     3181 tgatggagta actatagcaa aagagataga actagaagat agatttgaaa atatgggtgc
     3241 tcaattagtt aaagaagtag caactaaaac taatgatgta gctggagatg gtactacaac
     3301 tgctacagtt ttagcacaag ctataataag agaaggttta aagaatgtaa cagcaggggc
     3361 taacccaata cttttaagaa aaggaataca aaaagcagta acagtagcag tagaagaatt
     3421 aaaaaatcaa tcaagaatag tagaaacaca agaggctata tctcaagttg cttctatatc
     3481 tgctggtgat gaagaagttg gaaaattaat agcagaagct atggaaatag taggtaaaga
     3541 tggagttata actgttgaag agtctcaaac tatgaatact gaattagatg ctgttgaagg
     3601 tatgcagttt gatagaggat ttgtttctgc atatatggtt acagatgtag ataaaatgga
     3661 agcagtttta aatgacccat atatattaat tactgataaa aaaatatcta acatacaaga
     3721 attattacca gttcttgaac aaatagttca acaaggtaaa aagttattga taatagctga
     3781 agatgtagaa ggtgaagcat tatctacatt agtagtaaat aaattaagag gaacatttga
     3841 tgtagtagca gttaaagctc caggctttgg agatagaaga aaagaaatgc ttcaagatat
     3901 agcaatactt acaggtgctc aagtaatatc agaagaatta ggttatgatt taaaagaagc
     3961 tgatttatct atgctaggta gagcttcatc tgttaaggta actaaagaaa gtactacaat
     4021 agtagatggt tctggagata aaaaagctat agaagataga gtaactcaaa ttaaacatca
     4081 agtagagcaa actacttcag attttgatag agagaaatta atggaaagat tagctaaact
     4141 tgctggagga gtagctgttg taaaagttgg agctgctaca gaagtagagt taaaagaaag
     4201 aaagttaaga atagaagatg ctcttaatgc aactagagca gctgtagaag aaggaatagt
     4261 agctggtgga ggaactgctt ttgttagtgt tataccagca ataggaacac taattgagag
     4321 tttagaagga gaagttaaac taggtgctca aatagttaaa aaagcgttag aagagccatt
     4381 aagacaaata gctataaatg ctggtcttga aggtgctgta attgtgcaaa atgttgttaa
     4441 ttctgaagca gaaactggat ttgacgcatt gaatgaaaaa tatgtaaata tgatagaagc
     4501 tggtatagtt gacccaacta aagttagtag aagtgcttta caaaatgctg catcaatagc
     4561 aagtactttc ttaactactg aagctgctgt tgctgacctt cctgaaaaag aagatgcagg
     4621 aatgccagga atgggtggcg gaatgcctgg tatgatgtaa tttaaaaaat ttaaaatata
     4681 aattttaata taaagaacta tttcgactta gattttaaac tctaagttga aatagttctt
     4741 ttgtatatat ctaatttttg tatatctata aattcaatac acatatttag ataatcagta
     4801 tctagtttgt aataatattt tagagtagta ttgttatttt ttaggttatg attttagata
     4861 tatattaatt aaggtataat tgttgataga gaataaatca aatttaaaag gatgcgataa
     4921 tatggactta agaaaattgc ttgaagaagt taaaaatgaa aatattgata tagattttgc
     4981 gttagaaaag ttaaaagatt taccttatga agaccttgga tatgcaaata tagatcatca
     5041 tagagaacta agaaatgggt atccggaagt tatttattgt gaaggtaaga gtgatgagca
     5101 tattttaggg attattgata aaatgaatca aaaaagttct aacatattgg gaacaaggtg
     5161 cagaaaagaa acttttctga aaattaaaga aatctataat catgctgagt atgaagaggc
     5221 gtctaaaatt cttaagattc aaaatcatga tatagagaat ataggaaaag gtaaaattgt
     5281 gattgctaca gggggaactt ctgatatacc agtagcagac gaagcttatc atacagctaa
     5341 gtttttaggt aatgatgttg atagaatata tgatgttgga gtggcaggta tacataggct
     5401 tttaaataaa agacataaaa tagatagtgc tagagtaata gtagccgttg caggtatgga
     5461 aggtgctctt gcaagtgtaa ttggtggact ggttgatgtt ccagtaatag ctgttccaac
     5521 ttctgttgga tatggagcaa attttggagg attagcagca ttactagcta tgttaaatag
     5581 ctgtgcatct ggaatatctg ttgtaaatat tgataatgga tttggagcag gatatttagc
     5641 atcaatgata aataaactgt aaatatattg atataaatta aacaaaacta tataataaaa
     5701 tttaatttct attatatttt tactattaaa attgaggaga catatatgag tgaaaaaata
     5761 ctttattttg atataataag cggtatatca ggggatatga cccttgcaag tttattaaat
     5821 ttaggtgtgc ctaaggaaat ttttttggag gaaattcata aactaagaat gagcgatgaa
     5881 tttaatataa acatcagctc gaaaactgaa aatggaatag taggtacaaa agtagaagta
     5941 attacaaaag aaaaacatac acatagaaat ttagtggata tatttgaggt tatagataaa
     6001 agcactctaa acgaaaatgt aaaatctaaa gcaaaaaaaa tatttatggt aattggagaa
     6061 gcagaagcaa aagttcatgg aactacaata gacaagatac attttcatga ggtaggggct
     6121 atagattcaa ttgtagatat agtaggagct tctatattag tcgacttact ttgtgtagat
     6181 aaagtttgtg ctagtacagt gtctgttggt tcaggatttg taaaatgtga acatggaatt
     6241 atacctatac ctgcacctgc aactatagaa attttaaaag gtgtgccaat taaattaaat
     6301 aatgtaaatg gagaatgtac aactccaaca ggagctgcaa tcataaaagt tttgtgtgat
     6361 gagtttgtag atgagcttga attttctcca aagcaaattg gatatggtat aggtcataag
     6421 aagtttgaag taccaaatat actcagaact gtattaggtg aaaaaaaaaa gaagaattaa
     6481 tttatgaaat tagtgcaaac attgatgata tgtcttcaga aatctattct tatttgtatg
     6541 aaaaaatatt agatgagaaa gcattagata tttatacaga gagcatttat atgaagaaga
     6601 atagaccagc aacaaaacta tcaattttat gtaatgaatc agatttagaa aaatttatta
     6661 aaatattact tttagaaact agtacttttg gagtgagata taatgcatat aatagagaaa
     6721 ttctagagag aaaatttgta aaattagata caaaatatgg agtggttaca gtaaaactag
     6781 ggtattataa aggaaaacta gtcaaggcta ctccagaata taatgaatgt aaaactatag
     6841 caaaaaatga agatattcct ataaaaaaag tgtttgctga tataaatttt agaataaaag
     6901 aagaatttag tgaaaactta ttgacataag taataaattt tgataaatta atattcagcc
     6961 ttattaaata aaagtaaaca gaaaaaaata tataaaatat aaactcactc gtatatgctc
     7021 agaaatatgg tctgagagtc tctaccaaga taccgtaaat atcttgacta tgagtgaaat
     7081 tattatacca aaagacctag ttaagtaaat gtgtaggctg agttaaactc cctatgtgtt
     7141 ggctataaga taaaggtatt ttggaagcct gtatagtaat ttcacttttg ctagaaatta
     7201 tggaagttac tatacaggtt ttttgtgtaa aaagatttaa tataatttag actgtattgt
     7261 gttaaaataa atatataaaa actatattat acaaggagag atgtaaattt atgaaacatg
     7321 aattagtact tgtaatagat tttggaggac aatataatca gttgatagct agaagagtta
     7381 gagaaaacaa cgtatattgt gaaatacttc catgtactgc aagcatagaa agaataaaag
     7441 aaaaaaatcc taaagggata atatttacag gcggtccaaa tagtgcgtat ttagaagatt
     7501 ctccaacaat atcaaaagaa atatttgaac taggagtacc aatacttgga atatgctatg
     7561 gtattcaaat aatgtctcat gtattgggtg gagttgtaag aaaaggtaat aaacaagaaa
     7621 aagaatatgg aaaaacagct ataacttatg gaaaatcatc attatttgaa ggaataacta
     7681 caaatagtgt atggatgagt catacagatt taatagaaaa agttccagaa ggatttacta
     7741 tagttgcaaa tactaatgat tgtccagtag cagctatgga aaatgtagaa cgaaacctat
     7801 atggagttca attccatcca gaggtagaac actgtttgga aggggacaag atacttacaa
     7861 acttcttata taatatttgt aaagtaaaag gtgactggac tacagattca ttcatagaag
     7921 ataaaataaa agaattaaaa gaaaaaatag gcgataaaaa agctttatgt gctttaagtg
     7981 gaggagttga ttcttctgtt gctgcagttc ttattcataa agcaattggg gacaacttaa
     8041 cttgtatatt tgttgaccat ggtttactta gaaaaaatga aggaaatgat gtagaaagaa
     8101 tatttagaga gaaatttgat ataaatttaa taagagttaa tgcagaagat agattcttat
     8161 ctaaattaaa aggagtttct gaaccagaag ctaagagaaa aataataggt gaagaattta
     8221 taagagtatt tgaagaagaa tcaaataaat taggaaaaat ggatttctta gtacaaggaa
     8281 caatctatcc agatgttata gaaagtggac atggaaatgc tgctactata aagtctcatc
     8341 ataatgttgg tggaatccca gaagatgtag acttccaaga aattgtagaa ccattaagag
     8401 agttatttaa ggatgaagtt agaaaaatag gattagagct tggtatagaa gaaggattaa
     8461 tatttagaca tcctttccca ggaccaggtc taggaataag agttataggt gatgtaacta
     8521 aagaaaaatg tgatatatta agagaagctg atgctgttta tatggacgag cttagaaaag
     8581 caggtttata tagagaaata tggcaagctt ttgcaacatt accagatgtt aagactgttg
     8641 gagttatggg tgatgaaaga acatatgctt atttagttgg tttaagagca gttacttctt
     8701 ctgatggaat gacttctgac tggtataaga tgccttatga tgtattagaa agaatttcta
     8761 atagaataat aaatgaagtt gatggagtaa atagagtagt ttatgatata acttctaagc
     8821 ctcctggaac tattgaatgg gaataaaata tagaatgcca aaaatccaca taaatacagg
     8881 atttaaggtc tctcttactt ctatgttata ctggtttgat actatcaata tttgccagta
     8941 tcttatggat taataagcaa aaaacatcct gctgaatgat aaattcagcg gggtgttttt
     9001 attttgtttt agaactcctt tgctctgaaa ctcgaagtat ttaacactga tgattttgtt
     9061 atcttttagt tccttttgtg tcatgctctg aaagacaaat tgatacaata aaaaagtaat
     9121 ttcttaatca ttgatggaga tatgtattta tgttactctt gatggtaatt tgagtaatat
     9181 tgttcatagc tttttatatt cacttaatta gagaatcatt tggtataatc ataaataaac
     9241 ttgatgatat aatagaaaag atttataatt attagaagta tataaaagta ttttatgtct
     9301 atgaaatgta tgtctataaa acatatagat gacttttgta tattaatgta tgaatattga
     9361 tattatagaa gaattgagtt gtcaatgata ctaaatcaaa tatttataga agctggaggg
     9421 attatgagaa ataaaataat tataggaatt actttttcac ttgtattact aatgtctgga
     9481 tgtgcttcaa aacaaacagc agaaataaac gtattagcaa ctactgattt acatggagtt
     9541 ataccttatg agttaacttc ttatgtaaaa gaagaaagaa aaaaagataa aaatctaact
     9601 ttagtagatg caggagattt ttttgatagt ggagaagtgt ttggttctag catggatagt
     9661 tattttagtg ctaggtataa ggatattgat aataacactg agcgttacat tgagacacct
     9721 atttcaaaag atatgaaaga ggtaggttat gatgttgcag ttcttgggaa tcatgaattt
     9781 atatccaaca ataaatttta tctagataat atgatatctg attttgagaa gcaaaaaata
     9841 aatattttat cagcaaatac gtataaaaaa aatggagaca gttatacaaa accatacata
     9901 ataaaaaata ttaatacacc tgaaggaaac gtaaaactag gaattttagg gcttactata
     9961 aaagaagttg gtgagaggaa acagtggaaa gatggaaaac ttgttgactc taagtcttta
    10021 gaactaaaag accaagaggg ctacaatggt gaattgtata tgaatgattt agtagaagat
    10081 gctaaaaagt gggttagtat aatggaaaaa gagaagcctg acattatagt tgctgtggca
    10141 catacaggtg agaaacctaa gaaacctaga aacccaggaa atagaataca agacttagct
    10201 caacaagttg atggaattga tgctatagtt gcaggtcata atcatgttca aattgagcaa
    10261 catgattata aaaataaatc aggagaaaat gttatagtta ctgagccagg aaagcatgga
    10321 gaatgtattt ctaagattaa ttttaaatta gaaaaaaaca atgataaatg gaatatagtt
    10381 gacaaatcta gtaaattaat taaatttgaa aaaaatcaag aaagtgataa atttggagaa
    10441 tttatttctt caattaataa agtaagtaaa gataagaaag aaataagatt gtcagaagta
    10501 aagactcctg aatgggataa aatatatgtt tttaaatctg gaacatctgc agatgatata
    10561 tataaaacaa taggatataa atggagaaat atatcatctc caaagggtga tgacatggtg
    10621 cagattgttg taatgaataa taataagcct gttagctatg tatatggtga agctgaagtt
    10681 atgccaatta atataaaatg tgatgaatca gagtataaag ataatgttgt aactatttat
    10741 cctaataaaa atgataaatt taaaattgaa aaaggtaaac aagaatatag tattaattta
    10801 aaacatatac aatagaaact tttatcttat ttaagtttag attgtactta aaataaagtg
    10861 tgtattattt taaaaattga tgataattaa aatcacaaca ttaaggaata gtacacactt
    10921 tttatgctaa aaaatagaat gttaaaaaat taaaccttta aattaaagta cattgcttat
    10981 attatccaat attatcttca aaggttgtgt gatattttta tccttatgta atattgctaa
    11041 cattttattt tccatatcta tcccatctaa ttcaatcaat cgcaattttt tatgtaagag
    11101 tgaatctaat aataaatttt ctggcaaaat ggttatacct aaccctgctt ctgcagcttt
    11161 aataagtgca aaagaattaa cactttccca aacaggataa gctttttggt ttgaaagaga
    11221 taatacactg tccaaagtat tgcgaatggc acttccttgc tcacgtaaca ataacggaaa
    11281 cgtgcataac tgtcgtagag ataaggcttg tttagtattg gtggtggtta tgcaatcata
    11341 cctttaatcc aagagaaagt agtaaatcaa actggatgga taagtgagaa aatgtttaca
    11401 gatattataa ctatttcaca aatgacacca gggccactgg ctgttaatac ttctactttt
    11461 gttggacttc aaattggagg gttttgggga gcatttgcag ccactttagg ttgtgtatta
    11521 tgtggtgtag taatagctct atttttatat aatttttttc aaaagcatca gaactctact
    11581 tatatatttg aaatattaaa tggtctaaaa tcagcttcat taggtttaat tatctcagct
    11641 gcggctacaa ttataattct tgcattattt gggacgaata aagtgtactt caattctata
    11701 tttgagatgc taaactggac tgcactaata atcttttttg ttacactact gatagtacgt
    11761 aagtggaaaa ttaatcctat tgtgattata ataataagtg gtatactggg aatatttttt
    11821 tatttataag tttttgctaa gtaaaggata tctatttttt tatatcttaa atccaaatag
    11881 ccaataaagt aaggctattt ggatttttaa taaattctga aaaaataatc agaaacccat
    11941 ataacattta aattatatca ctggttttct acaaaccaat cataagcttc ttggcaaaat
    12001 ttctcactat ctatatagta actattatga acattattaa tttcaacaag tttcttttta
    12061 cattttagtg aattataata tctatcaaaa tattgggttt taaaatatct atcatgaagt
    12121 gtcttaaaga caagagtagg tatgttgact ccatctttta tcttactatc ttctgcaata
    12181 aagcattcta acattgtatt aattatcttt gtactatttt tccaaatatg aggatttttc
    12241 ttttcaaata ttttataaaa ttcattatca tagacttcat tataaccttt atatttaggt
    12301 ggttttactg ttatgtgaaa attaggaaga aacttaaaga aaaagtttaa taatttaaca
    12361 gtgagatagg tatagcctac tcctgttgtg tttttataat caacacaagc aaactgagca
    12421 aaacttttta acttattgtc tgaacatgca taatattgtg caaacatacc accaattccc
    12481 gtattcccat atagatgtat gttggaagaa taattttttt ctatgtatgt aatgacagaa
    12541 tctaagtctt ttacaattga attgcgagaa aaatcttttt catatccatc acttttccct
    12601 gttcctgaaa aatcaaaagc aaatatattt acattatgtt tggcaagtgg ttcatatgct
    12661 ttttgcatct caagcgtagt gacaatagga gtatgtgtaa caacaatatt catggatgat
    12721 tttctggtaa taaatgagtt acaaaaaatc attgtatctc catttggtat ttttatatgt
    12781 ttccaactca tttgattttc ctttctctat tataaaatat aataattttt atgttctgtt
    12841 ttatatatta ttcatatttt atcacagata atatactaaa gttaattttt ttattgtaca
    12901 cctattataa taattcttct ataatataag acattctata attttatatt taagttcatg
    12961 tgtatttaat tgtttttata taaaatgtcc agtaatacta tttggattta tttttatttc
    13021 atcaggagtt ccagaagcaa caatttcccc tccatttatt ccactgtctg gacctatatc
    13081 gataatccaa tcagaagcac gtataacctc tgtattgtgt tcaataatta aaagtgaatg
    13141 ctgttcattt acaagttttc taaaagtaat taacaaattt tctatatctt taaaatgaag
    13201 tccagtagta ggctcatcca atatgtacag cattcgtttt ttagtttttg tccctaattc
    13261 ttttgctaat tttatccttt gagattctcc tccacttaat cctaatgtat tttgtccaag
    13321 ctttagatat ccaagtccca cttcatctaa taaggagagt tttctatata tatcagagca
    13381 atcgctaaaa aatgtttttg cttctgctac ttccatgtca agtatatctg caattgtctt
    13441 tcccttgtat tgtatagaca gtatttcttt ttgatatctc ttaccttgac attcactaca
    13501 agttacccat tggtctgcca tatattgcag aataattttt gttttaccta atccttgaca
    13561 gtttgggcat tgcccctttt tgctattaaa gctaaaatag ctttcatcca ataatttttg
    13621 ttttgcttgt ggagtttcag caaatagaga acgaatttca tcaaaaatat tgatatatgt
    13681 agcaggtgta gaacggctag attttccaat aggtgtttgg tccattaaga taaaatcatc
    13741 aatagcatca aatccagtaa attcagtata atttttatct gggatagatt tttgtttcaa
    13801 tttttcttct agagcaggaa gtaaactgtg aaatacaaga gagctcttac cagaaccact
    13861 aacacctgta atagagcaca tacagtttaa gggaatttct acatcaatat tttttaagtt
    13921 atttgcatgg catcctttta agcttaacca tttacttggt atagttttat tttgtgaacc
    13981 tgttttattg tatgtactta aatatttacc tgttatagag tttggatttt ttataatttc
    14041 ttgggttgta ccttctgcaa taatgaaccc cccttttgta ccagcacttg ggcctacatc
    14101 aataatataa tcagcagaca gtattgtatc tttatcgtgt tcgacaacta taacagtatt
    14161 acccttatct cttagctctt ctatcatttt gatgattaaa ttatggtctc ttgggtgtaa
    14221 accaatagat ggttcatcta aaatgtaggt taagcctact aattcacttc ctagctgact
    14281 tgataatcgt actctctgca gttctcctcc agataaagtt ggagatgttc tatctgttga
    14341 tatatagcta agacctacct ttaatagata actaactcga atttttaatt ctgttaagat
    14401 atcatttacc aaagagagct catttttttg aagttgattt ggtaattcac acaaccaatt
    14461 ccataactgt tctattgtca tttttgttag ttcagggaag cgataacctt tgattgtagt
    14521 gaatctagcc tcaatgcaca gcaattcgcc accacatgta ttgcaaggaa ttttgttcat
    14581 atattgatgc aaggtattat tttcagaact agattctcta aaaagtcttt gaatgtggtt
    14641 gattgctcct gaagcaggtc ttctaatctt tgattctcgt ccctttgatt caaagctaaa
    14701 ttcatatatc ttatcgtctg taccatataa gattgcatct ataaaatctc ttggtaattc
    14761 attccaaggt atatctaggt ctatattttt atcttttgct attgcatata attcacctac
    14821 catccaatta ccatttggct ttttccccct caattttcca aaatagtaag tggctccatc
    14881 taatattgaa atagttgggt cttttacaat caattgagga ttaacttgat aggtaaaacc
    14941 taatccatta caatcgtgac aagctccagt atgggagtta gcattgaagg ttgaagtatt
    15001 tatcttagaa aaaatagttt gacaatgcgg acaataattt tttgtattat tctttttttc
    15061 taatggaatt ttacaatatg gacagtaact ttttccgatt gatacaaata acattcttaa
    15121 ataatcccaa atgcttgtaa gagtaccaac agtagagcga ggattttggc ttcctttttt
    15181 ctgttcgata gctatagtag gggttaatcc ctgaagtaaa tcaaattccg gtttttgaag
    15241 ctgttctgaa agttttgctc cattacttag attttcaaaa taacgtcttt ttccttcttc
    15301 ataaatcgta tcgaaagcta aagaagattt tccactacca cttacacctg taataacagt
    15361 tatttgattt tttggaattg taacctcaac atcctttaaa ttgttttgcc ttcctccttt
    15421 taaatagata gagcttttat tatgacaaag tgaattttta attgatttat ttattgattt
    15481 tgtaaatttg aatttctttt ctagttcaag aggattgcct aattctttta tttccgaaat
    15541 acctaatatt tttaactgag aaaagattga tttcaaataa tcttcaggca atttttctat
    15601 taacaaatta aggtcttcta tttttatatt tccgcttgat aatatttctg ccatttcatc
    15661 taataaattt ttttctgctc ttgttaattg attgtatttt ttggaactta attctgtcca
    15721 taaatcccat ggaagcaatt ctaaaccaat catattatta aagtcatgta gaagattacc
    15781 ctttacagaa tgccaacctt tccaattttt attatatcca aagtcatctg gatgagcatg
    15841 tccttgccga catcttatcc aggcttctga accagttaaa aaaccatctg tttttccgac
    15901 atcatgagtg tcaaaattaa tttgaaaata atttttttgt atgtcatcta tttgtgcatc
    15961 tattcttatc catctctttg ttagagtatc atagtattct acaagccagt ggtcttcata
    16021 agtaagctca ctttcaaaat aattagcaaa acctgctctc attctagcct caaaacctcg
    16081 atatcttagg agcgatacaa gaagtaaaga ataatctctg cacattccaa caagcctttg
    16141 ttttgggttt cttggaatag ttagtgggtt agaatttatt tcaaatattc tttttaacat
    16201 ttgaggaatt gtacgaagca attcctcatc agattgttgc ttattaaagg atataccata
    16261 taattttcct tggtctccat gaattactag gctttgaacc acttttaata actcaattgg
    16321 agtctttggt aaatcttgat atttattttc aaatttttta ggatttgtga tgatgctttg
    16381 atttttatag ttatttattt tcatttatat ttcacctctc taaatggatg atataccctg
    16441 acatggtgtc atatgcaagg acaatttaaa ttttttgtcc aaaatttaaa aataaataaa
    16501 aaatgataca gtgataaatt gtaatacaaa tatagtttca aattgcatat aatataatta
    16561 tttaataaaa ccattatcta tggaatgtta atgaaatgtt aagatggcat tcttgaatat
    16621 taagaaatag aatactataa tatagtgtga aggattctta gtaaaaaaat ttgtaagtgt
    16681 taatatacat taaaaaatag gtttataatt tggaggaaaa aaatgaaatt atcaaggtat
    16741 aagaaaaata gtttagttct gtctataatt ttatgttcac ttattatttc aatccttgta
    16801 attactgttt ttaataccgt aaaactacac aatatatcta ataaacaatc taaaaactat
    16861 ttaaatgatg tatctacaca gattgtaatg aatgttgatt ctaaaatcaa atttatttta
    16921 tccgacttaa gaataatggc agattttatt aagcaatatg aaggtgatag tcgatatgag
    16981 tatttatcta aaagaaagac tagttataag tattatgatg ttggaataat agatttagaa
    17041 ggtaaagctg agtttttaag tggaaaaaag tttgacttaa agaatactgc ttcctataaa
    17101 aaaggaattg aaggtaaaga atactcagaa gtaatagagt caatgggttt tgttctttat
    17161 agtgtaccca tatttaatga taatcatgaa gtatctagta ttttaatagg tgtatccaat
    17221 aaagaaagta tgaatgagat tcttaatata gataacttta atggagaagg aactattgaa
    17281 attattaata gtaagggaaa acctcttttc ataggaaaaa atactgaatt aataaaagac
    17341 cttagccata aatataagga ttctacagat gaaccttggg cacaaaagat gcttaatgat
    17401 tttaaaaatg ataaatcagg gaatattaca ataacctcat caaaaggtgt acaatgctta
    17461 ttgacttatc atccaattac aagaggttta aatgattggc attttcttct catagtacct
    17521 gaagatgctg tacttggtga acttaataag ttaaattctt ttacaataac aatgacattt
    17581 ataattacat gtattattgg aattataaca ttaattttat atattataag aagaaaatat
    17641 gtaaatcaaa tagaaaatat tgcatattta gatagtataa caaatggcat aaattcaact
    17701 aagtttagta tgctagtcaa acctttaatt tcagaatcac ctgatagtac ttatatgatg
    17761 atagcaatga atataaaaga ttttaagcta ataaatgatt gtttcagtag tgaaaaggga
    17821 aatatgacac taaaacatct ttacaatatt ttgtataaaa acattgatga aaatgaagaa
    17881 tttgtgtgcc gtcatgatgc agaccttttc tatttgctta ttaagaatag accagtgtta
    17941 gaagccctgg agttattata taaaatagaa gctgacacaa attattttaa taaaaatagg
    18001 gaaaatccat actttttaag actatcagta ggtatttaca caattgaaaa tcataatgaa
    18061 gatttaatta caatacaaga ccatgcaaat acagcaagaa aaagctttaa taaaactcat
    18121 caaagtgatt tttcttttta tagtgatata gaaagaaaac gattaatatc tgaaaaggaa
    18181 atttcaaatt taatggaaaa agcccttgaa aataaagaat tttttatgtg cttacaaccc
    18241 aaaattgata taaaaagtgg aaaaattagt ggagcagagt ctcttgtgcg ttggaaaaat
    18301 tcagaaaagg gcataattta tccaagtgat tttattcctc ttttcgaaaa aagtggattt
    18361 atatgtaagt tagatttata tatactagaa gaaacatgca aattgatttc taagtggata
    18421 aaagaaggta aagaagtgtt gagaatttct gttaatgtat cacgtcaaca tctgaatgat
    18481 aaattgtttt tagaaaaata taagcatata tgcaataaat acaatgtacc aacatgtctg
    18541 atagatttag aacttaccga atctattttc ttagaaaatc cagaagctat agatataata
    18601 gaggatattc attctaatgg atttaaatgt tctatagacg attttggatt tgggtactca
    18661 tcattaggaa tattaaagga ttttaaagtt gatataatta agttagaccg ttcattcttt
    18721 gttagcaaaa ataatataga ccgtggtaag gtagttatta agtctattat tgaacttagc
    18781 aaaaggctag gtatgaaagt aactgctgaa ggaattgaag aattagaaca agttgagttt
    18841 ctaaaaaatg tgggatgtga ttatattcaa ggttatgttt tttcaaaacc attattaatt
    18901 tctgattttg aaaattttgc atatgaaaat aataaaatta aaaatatcca ttattaaatg
    18961 tacaagataa aaaatttagg caagttatat aattaaaaat ataacttgtc ttttttattt
    19021 atatatttaa taaaaatata tttgcagatt ttgtaaaaat aattttcaaa tgttgtaagt
    19081 tgtggctaaa gctttattgt attattctga atatagaaag atagttactg tcttagataa
    19141 aaactgaaac atacataata atataaaatt tgctatatta ataactctaa ttattttgga
    19201 aggagggaaa atatgataag tgggagaatg ttactcatca tgaattttct aaaaggaaaa
    19261 aataagacaa gttacaagga tattagcaaa caacttagta tagaagaaag aaaagttaga
    19321 tatgatataa ataatttaaa tattgttttg cgttcattaa acaagtcagt aatacaaaag
    19381 atgaacaaag gtgtgttaat tattcctgat gacttccaag tagtatgtat aaataataac
    19441 gaatatgtat tttccttaac tgagcgagta tctattatga aaattatatc aatgtttaaa
    19501 atagataatc taaacttaga aaaattaagt aaagaatttc aggtttctag aagttcaatt
    19561 aaaaatgacc ttaatgtttt gtcaagtgaa ttagaaagaa accaaataac tattacttat
    19621 gataaatctt ttaaaataag tggagaagaa gacattatat ataagcaaag attgaacata
    19681 ttaaaatcgt attcttatct ttttggtaag gaaaaatctg atttaagtgt atttgagaag
    19741 tatttagtga atgtaattga aaaaatattc aaagggattt atttgattga tatatataat
    19801 tggtctatga atttattatt gcaaatggga tggactttaa atgatgaatc ttttacatgg
    19861 tatgtgtcaa atattttcct ttttacatgg tatatacatt cagatgaaaa acatccttta
    19921 gaatctgctt cattaagtga gccgtgtttt gaaaataagt ttgaccaaga atttgaaaca
    19981 attattcaaa aaaagttgac taaggaacaa atttttctgt taaagagttt tgtatttttt
    20041 acaaataaat atgcaagttt aaacgaggat atggatttga ttagtacaga aattatagtt
    20101 caagatctaa tttacaatat gtcagaaata ttagagatta gctttaaaga agatatgatt
    20161 ctttataaag gattactcaa tcatattgcc ccattgatag agagaatcaa acagaatata
    20221 cagatttatg aggaattact tcatgtaata ccagataaat atcaatatgt acttgaagct
    20281 actaaggttt ctattcaaaa aaacagagtt ttaagtcaaa taaagaatga aaatgaaaca
    20341 atacttttgg caatacattt cttaggaagt atacatagaa atgagaggaa taactacaaa
    20401 aatatattgt tagtttgtgg atttggatat ggtgtaattg ctatgttaaa ggatagttta
    20461 gtaaatgatt accagataaa tatagtagag agtattccat catataagtt acaagattat
    20521 aaagaatggg aaagtattga cttcattata acaacatcaa agatagtaat agacttgcct
    20581 aaaacaatta ttgagataaa tccattttta caagaagagg attatttgaa gtttgaagaa
    20641 aaagggataa aaagaaagaa cgtcttaact aattatatgt caataaaaaa aaggttggac
    20701 tttttagaag aagatgccca aaaaagagta ttaagtatta tatgtcaaga acttggatac
    20761 agtgatgaga gaatattatt tagacaactt aatttaagtg atttaatagg gattgatacc
    20821 ataaggataa ttgatggaga tatgaaatgg gaagatgctg taaaatttag ttcaaacata
    20881 cttgctaatt gtgattttgt atcagcacaa tatgctgaga atattataga tattttaaaa
    20941 aatgtgggtt tttatgctgt aaaagataac gaatttgctt tactacatgg caatgatagt
    21001 tcattagtca aagtaagttc gataagttta ttaatatgta aagaagcagt tagttttgga
    21061 gataaaaagg ttaaaatcat attttgccta gcaagcagag ataaaaaaga gcaaattcct
    21121 gcaataatta atctaactaa aatggtttat aagactaatt ttattgctat gttagaaagt
    21181 gcaaagacaa aagaagaagc agaagtttta attcataaat atgagaagga ggtcactgta
    21241 tgaataaatt agttaatgta aaatgttcat ttaaaataca tgttaattca aaagaagaag
    21301 ccattatatc gttagttgat gtagtgtcaa aagaaggata tttaatagat aaaaatcaat
    21361 ttttaaaaga tgttttaaag agagaagaaa cattgtcgac ttatattgga catggtatag
    21421 gtcttcctca tagtcaaagt gtaggtgtta aaaactcttg tattacaata ggaaagcttg
    21481 atactcctat tgaatggaca gaagaaggag aaaaagtgga tttaatattt ttgatatcag
    21541 taacaaaaga caatgaaaat aatcttcatt taaagatttt atcaaaatta gcacgtttat
    21601 tgatgcatga aagttttaga aatcaaatta gatgttcaga tgaacaaact gtttataatc
    21661 ttataaaaga aaaaatagag gaggaagatt aaaatgagaa ttttaaagtt tttgcaaaaa
    21721 catcttatga cagcaacatc ttatatgatt ccatttgtgg ttgcaggagg aatattattt
    21781 gctttatcag taactctaag tggacaagct gctgttcctg aaacaggctg gttggctaaa
    21841 ctgaatcaaa ttggagctgc tggcttggct ttattcattc ctatattagg tggatatata
    21901 gcatttagta tggcagataa accaggatta gctccaggta tgataggtgc atatttagca
    21961 aaagaagtaa atgcaggttt tataggtggt atcatagcag gatttatagc aggatttgtt
    22021 gttttacaat taaagaaaat taaattagct cctacaatga gaactttagg ttctatattt
    22081 atttatcctt tgattggtac attgattaca ggtggtataa ttgtattttt aattggagaa
    22141 cctatagctt cttttatgac atggatgaca aactggttaa atggaatgtc tggtgtttct
    22201 aaaattcctt taggtggaat cttaggagga atgatagcct cagatatggg tggtcctata
    22261 aataaggttg ctgctacttt tgcccaaact caagttgaca cacttccata tttaatgggt
    22321 ggagttggcg ttgcaatttg tatacctcca attggtttag gattagctac attattgttt
    22381 ccaaaaaaat ttagcaagga agaaagagat tcaggtaaag cttcactatt aatgggatgt
    22441 gttggaatta cagaaggagc tattccattt gcaacatcag atccagttag agtaatacct
    22501 tgtattatgg ctgggtcgat tataggaaat attatggctt ttttattagg atgtttaaat
    22561 catgcaccct ggggaggcct gatagtatta cctgtagttg acaataggtt ggggtatatt
    22621 gcatcagtat taactggagc agtagttgtt gcagtattaa tgaaattagt aaaaaaagac
    22681 gtgaaagaag atgaagaaat tgaagaagat ttagatgaat caattgaatt aatatttgaa
    22741 gaattataaa tataaatgga aggaatttgt ataaaaaagt aaatataaaa ctataaattt
    22801 aaaggagata agaatatgaa aattgtagca gtaacatctt gtcctagtgg agtagcacat
    22861 acttacatgt cggctgaatc acttgaatta tcagccaaaa aatttggtat agaaataaag
    22921 gttgagactc aaggaagttc tggaatagat aatgaattgt cacttaaaga tattgatgaa
    22981 gcaacttgtg taatattgac taatgatgtt gagattagaa acatggaacg ttttaaggga
    23041 aagaaggtag taagaatgag tgtatctgag ataattaaga aatctgatgc attaattaag
    23101 aaaataaaag atacttttca ataaaaattg atttatttta catataactc aaagatgttt
    23161 ttaaagaaat ttttattaaa agcatctttg aaaatatctt aaaaatagaa taaaaggaga
    23221 tatagtcatg aaaaagttac caataaagac tgtcgtacaa agattactaa acttacaaga
    23281 agaggggaaa agtgcaactc ttttaggaat tggtcctatg tcaccaaact tgttacaagc
    23341 aagttttgaa ctagcaaaag atgatgattt tcctttaatg tttatcgcaa gtagaaatca
    23401 agtagatgca gatgaactag gtggcggtta tgtaaatggt tggaatcagg aaacttttac
    23461 aaaagatatt aagaaggttg ccgacaaagt tggatttgat ggtttatatt atttatgtag
    23521 agaccatggt gggccatggc aaagagataa agagagaaat gaccatttgc ctgttgatga
    23581 agcaatggag ttaggaaaaa aatcttattt agcggacatt gaagcaggtt ttgacctatt
    23641 gatgattgac cctacaaaag acccatttga aattggaaaa gtaattccac tagatgtagt
    23701 actagaaaga actgtagaat taattgagta ttgtgaaaac gagagaaaaa gacttaatct
    23761 accagacata ggttatgagg ttggtacaga ggaaaccaat ggaggtctga cttcaactga
    23821 gacgtatgaa acatttataa ctagactaaa ggttgaactt gataatagag gtttgccaat
    23881 gccaacattt atagttggtc aaactggtac tttaactaga aagacagaac aagtaggaat
    23941 ttttaatttt agaaatgctt atgatttagc tcaaatggct aagaaatatg gagttggttt
    24001 aaaagagcat aatggcgatt acttagatga tgtaacttta ttagaacata taccatctca
    24061 aattattgct acaaatgtag ctcctcaata tggtactgaa gaaactaggg catatttaaa
    24121 attagcagaa gttgagtgta aattagaaaa agagggctta gttgagaaaa cttctaatat
    24181 cagacatgtt cttcttgtta atgcaattga atgtggacgt tggagaaaat gggtagtagg
    24241 agaacaaaaa aatctaacta ctgaagagat atttaaggat gaagttttat ctaatgaaat
    24301 actagatatt gcaggacatt atacatttaa taatgatgat gttaaaaaag aaatagaagt
    24361 gctttatgat aatttaagta agaataatat tgatggtcaa agatttgttg tagaccatat
    24421 taaacgtcct ttgcgtgatt atgcagaatg ctataactta aaaggtgtta ctacgaggat
    24481 tttaaataaa taataggact tatagtggtg agtattgtta tactaggtat ttatagttat
    24541 aattatactt accactatta aatttaggag gactaatatt ttgattaatt taatttttga
    24601 tgtagatgat actttatata atcaattaac gcctttttac actgcatata ataaagtgtt
    24661 ttcttcaata aaggatattt ctattgaaga tttatatatg agtagtagaa agtatagtga
    24721 tgaggtattt catatgactg aaaatggaga aatgccaatc aaagaaatgc atatttatag
    24781 gataatgaaa gcttttgagg agttgggaaa ttctattaca gaaaaagatg cacaaagttt
    24841 tcaagatgag tatatatatc aacaatcaca aattacacta ataccagaag ttgagaggat
    24901 tttaaatttt tctaaagaaa gaaatattaa tctaggaatt ataactaatg gcccatcaaa
    24961 ccatcaaaga atgaagttaa agcagttaaa tattgaaaac tgggttgata aaagtaatat
    25021 ttttatttct agtgaggttg gattttctaa gccagatact aatattttta gagtagctga
    25081 gaatgttatg aatttagata gagaaaatac atattatgtg ggtgattctt atagaaatga
    25141 tgtattaggt gctaaaaaag ctggttggaa gagtatatgg ttgaaccata gaggatatga
    25201 ggtagaagaa ttgttttata aacctgattt tgtaattttg gaacataagg atttgatatc
    25261 tttatttata aaaatatgta gttataaaaa tatgtagtaa aaaaatgtta ataatagaat
    25321 tgtataaaat aattaataat aactatataa aattatatgt ttagattgtg agcaatctat
    25381 tttatattaa aaatagatta tttagaagaa ggggaaatct taaaaaaatt tgaatatccc
    25441 ctttttatta tttaattaaa atgaaatagt tagaaatata ttttatttag tttagtcaat
    25501 aacattttgg gattctttat aactacatgt taactgttga ataggtttat catatggttt
    25561 aacccatcct ttattaatac ttaattctgt aagtgctgtg tgaccttcta ccatttgagt
    25621 taatgaagct gaatatatag ctcttaattc aggtgtacta cttgtcaatg cagaattaag
    25681 atacatattg gcagatgctt tagcagatga aagcatactt aacattatta ctttatcgtc
    25741 tatataagta ttgttcttaa ttagattttc tatcatattt ctcatgttat ctatacctcc
    25801 tcagaaatta gtatttcatt ttcaactata aattgttgta tagctttaat tcttccttct
    25861 gttgctaata tactagattc tgattgtctt tttaggtctt catctgatat taacatattt
    25921 attgctcttt gcataagtaa tccgtcagac tccattttta taattgctgc taaagatatt
    25981 ttttcagttt ctgaaagtat tctcatttaa tcatctccta tattgtaatt tatgctttaa
    26041 tagtatgtct ttcttttcaa ggaaataacc attacacttt tttacagtat tttatattct
    26101 ctttatatca aaatttagtc gacctaatat attaatataa ttttgtagga tatttatcat
    26161 taaatatcta taaaataaat tatttgaata ttcataaagc aaaatttatt tgtatgtact
    26221 tcttcaagag tcaatatgag ttttttcaaa aaattaattt ttatgataat cattaagggc
    26281 tatatttttc ttaaagttgt gattatcatt attaatttac aataaaattg ccgaaaagcc
    26341 ctctccttta ggtgtaggga tgaaaggtaa ggtcttaata taaaaaaagt tagaataatg
    26401 cttgtaatct aataccgcta ctgataccct atatttagg
//
LOCUS       DAGSRM010000046        25960 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.046, whole genome shotgun sequence.
ACCESSION   DAGSRM010000046 DAGSRM010000000
VERSION     DAGSRM010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25960
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.046"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            128..1387
                     /locus_tag="MJE33_002117"
     CDS             128..1387
                     /locus_tag="MJE33_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBY9648050.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKAFGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKALDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKDKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLVLVGFSMSKGFTAYGMRMGAIICIS
                     SSEDVAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVKEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINN"
     gene            1448..3235
                     /gene="iorA"
                     /locus_tag="MJE33_002118"
     CDS             1448..3235
                     /gene="iorA"
                     /locus_tag="MJE33_002118"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HBY9648051.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKEYVKNAKKMVTVPANAQIRRGVVEERMEILKKFSNET
                     DLNYYEINDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTNDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDDNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVDHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            3237..3812
                     /locus_tag="MJE33_002119"
     CDS             3237..3812
                     /locus_tag="MJE33_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HBY9648052.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELSRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            3865..4935
                     /gene="buk"
                     /locus_tag="MJE33_002120"
     CDS             3865..4935
                     /gene="buk"
                     /locus_tag="MJE33_002120"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBY9648053.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLNILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDKGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGQYSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGKVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            5153..5854
                     /locus_tag="MJE33_002121"
     CDS             5153..5854
                     /locus_tag="MJE33_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648054.1"
                     /translation="MGKISLKSMIRLFSGFFIFAISSVLMINAHVGLMPWDVLHQGLS
                     IKLGITIGQASIMVGVVIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHASN
                     TYIGVFMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEILA
                     LVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKKSN
                     EHKCKSSIVIKNEQN"
     gene            5914..6420
                     /locus_tag="MJE33_002122"
     CDS             5914..6420
                     /locus_tag="MJE33_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBY9648055.1"
                     /translation="MELWDLYNADGIKTGNVIKRGNSIEEGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEDSLEGAIREAKEEIGIDITKDEMKVFRSMIH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEIYELLYE
                     IDNNKLNI"
     gene            6595..7617
                     /locus_tag="MJE33_002123"
     CDS             6595..7617
                     /locus_tag="MJE33_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBY9648056.1"
                     /translation="MEDNKGLARDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSSLNIPDSLIIIVLYALFAILLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKNFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMGFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAVETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(7764..8072)
                     /locus_tag="MJE33_002124"
     CDS             complement(7764..8072)
                     /locus_tag="MJE33_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBY9648057.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINERNASCETFEMK
                     "
     gene            complement(8132..8560)
                     /locus_tag="MJE33_002125"
     CDS             complement(8132..8560)
                     /locus_tag="MJE33_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648058.1"
                     /translation="MNNKLHELEKNLPEISCSFCTHLSFKGPSLDYRYDIRCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFKCSICGSTENVDVYHVNKNLGRETSKDVVVKCDKCNCK"
     gene            complement(8783..10240)
                     /locus_tag="MJE33_002126"
     CDS             complement(8783..10240)
                     /locus_tag="MJE33_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBY9648059.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGLIFLYYLCATLFPIDKIIGKVYPIFGAILLLSAAGVGIGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELVGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIIKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNI
                     NISYVLAAIFTVLYAILTVRSGRKLKSKADTKLAD"
     gene            10658..11572
                     /gene="nadA"
                     /locus_tag="MJE33_002127"
     CDS             10658..11572
                     /gene="nadA"
                     /locus_tag="MJE33_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBY9648060.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKILSPEKTVLMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKNFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYSTLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            11585..12886
                     /locus_tag="MJE33_002128"
     CDS             11585..12886
                     /locus_tag="MJE33_002128"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HBY9648061.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMKAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFIDVPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            12867..13703
                     /gene="nadC"
                     /locus_tag="MJE33_002129"
     CDS             12867..13703
                     /gene="nadC"
                     /locus_tag="MJE33_002129"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HBY9648062.1"
                     /translation="MNYLIIDRMIQDALIEDVPSEDITTNSIVDENSKSTVDLICKQD
                     GIIAGLGVFKRVFEILGDVDVKLYKNDGDRVKNREKIAVLTGSTRNLLVGERVALNYL
                     QRMSGIATITSRYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFNLSDGI
                     LLKDNHIGAAGSVKKAVELARKNTSFVRKIEVEVETLDMVKEAIEANADIIMLDNMSL
                     EMAKQAVDIINGRAIVEFSGNVNLDTIEDIGKIGVDVVSVGALTHSVKALDISMKNLR
                     NI"
     gene            13959..14291
                     /locus_tag="MJE33_002130"
     CDS             13959..14291
                     /locus_tag="MJE33_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBY9648063.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPVGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
     gene            14453..14638
                     /locus_tag="MJE33_002131"
     CDS             14453..14638
                     /locus_tag="MJE33_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648064.1"
                     /translation="MSEKYADKLEIKLYQAGKDFSYIKKYGIITKGTLIINQKKKYDR
                     LNKDTIERAIVEAINNN"
     gene            14665..15750
                     /locus_tag="MJE33_002132"
     CDS             14665..15750
                     /locus_tag="MJE33_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBY9648065.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGPKVTIIYLIAGFLVPFLVGFLGNCFGKEELSLHNDSEEEVIEL
                     EEEKRSFMENMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQKYLGNPGL
                     LSLGSIAILACVMYVCAVGHIPFIAALVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSIIPKMKEVIGNHA"
     gene            15743..16201
                     /locus_tag="MJE33_002133"
     CDS             15743..16201
                     /locus_tag="MJE33_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648066.1"
                     /translation="MLKKIVIISCIVVVLIILSKIVDDNIKEDASIPNVNKETLEYFR
                     KNYKEDIITCAEEDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIEN
                     QTITFKNIDDKAPIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            16236..17327
                     /locus_tag="MJE33_002134"
     CDS             16236..17327
                     /locus_tag="MJE33_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBY9648067.1"
                     /translation="MKLSKKIQALILFGVMGTSILTGCSSKPKEKEEAKTGKDDYTIT
                     VGYYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGA
                     IPKGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLP
                     TDPSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSVAENDGAGKIIASTQYKEKAN
                     GKEYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVEEEV
                     ALRTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGA
                     DDFDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            17332..17556
                     /locus_tag="MJE33_002135"
     CDS             17332..17556
                     /locus_tag="MJE33_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648068.1"
                     /translation="MENMKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDTS
                     GFVINYMVNRDYFENIKVSDIMDIFTINDC"
     gene            17566..18129
                     /locus_tag="MJE33_002136"
     CDS             17566..18129
                     /locus_tag="MJE33_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648069.1"
                     /translation="MQWALSSEEIDIAIICKDAAKQYVNVNSGFEIVGTVVQNSDIFL
                     LSDNNPEKIGVIQNRDYQSELVKKYYKNVEVAPLLGTALAYGLESNLVDGVVIDCIKS
                     IGLEGKRKSTTKLGDYDTYVLVVSKEFKKSKAYSDFITLYNKSVDALKNKATFKKALK
                     EYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            18090..18719
                     /locus_tag="MJE33_002137"
     CDS             18090..18719
                     /locus_tag="MJE33_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9648070.1"
                     /translation="MEIKISKDKSDRLTKKKTDGSMDILYKIILLVIIFLVWHFAAKD
                     IGSSLLLPMPVDVIKGFFFCVTDAETVTNLFITLQRVLKGFMYALLFGLPIGFIMGFS
                     KTFERVLSPVVDSVRQVPIMAWVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVR
                     AISKDYYNAARSMGASPIVIFTNVIVPASLPDILTGSRIAISTGWMSVI"
     gene            18896..19648
                     /locus_tag="MJE33_002138"
     CDS             18896..19648
                     /locus_tag="MJE33_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9648071.1"
                     /translation="MKLSVRDINKTFVNNRVHTKVLEDISMDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSERGDVFINDKRVKKPGIDRAFIFQNYALFPWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEVLLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            19661..20146
                     /locus_tag="MJE33_002139"
     CDS             19661..20146
                     /locus_tag="MJE33_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648072.1"
                     /translation="MSQIEKYNSLDNTQQKIRISIMDMIIDKGSSVTLQEVTEYISKK
                     LNIEKEYIERTLQYFIYKNIMVVDGNSINFIYPVSALPTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDIKINSICSATGKEIEVIIKNEKIEYVNNPNLRVLHINLDKHLNWAAS
                     C"
     gene            20153..20302
                     /locus_tag="MJE33_002140"
     CDS             20153..20302
                     /locus_tag="MJE33_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648073.1"
                     /translation="MNFFWTKSDFDAWTNEAGLSNDEDIYCLDINEAIVESYKIFKLK
                     QKVLS"
     gene            complement(20439..21260)
                     /gene="yidA"
                     /locus_tag="MJE33_002141"
     CDS             complement(20439..21260)
                     /gene="yidA"
                     /locus_tag="MJE33_002141"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBY9648074.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMSLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNETPGKYTLHEAKLNNIEVKYIQKDKIDESIKICKIMIVDEP
                     ERLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCTNLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
     gene            21753..23447
                     /locus_tag="MJE33_002142"
     CDS             21753..23447
                     /locus_tag="MJE33_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBY9648075.1"
                     /translation="MKFSEFKYERPNYNSMKKEFLSCVEDINNSRNYKEQQKNIHKIN
                     LLRNKIETLSNIASIRYSTDTFNKFYKEEKNYWDEYMPLYEELNSYFYNAIVNSKFKY
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGKIRNLS
                     GMGKFMYSKNRKTRELANKAYYNFFEENETKFDDIFDKLVKLRDKMSKKLGFEDFVEL
                     GYVRMMRSDYREYMIKNVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIKNGKRMYSELSKETNEFFDFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKFTHLSSSIKFIPYGVIVDEFQHYIYKNPNVDKSKRKEIWR
                     FLEKKYLPHRKYGDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DAGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
     regulatory      23779..23889
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            23993..24364
                     /locus_tag="MJE33_002143"
     CDS             23993..24364
                     /locus_tag="MJE33_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HBY9648076.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQNNKKTPKWTESVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            24461..25408
                     /gene="trxB"
                     /locus_tag="MJE33_002144"
     CDS             24461..25408
                     /gene="trxB"
                     /locus_tag="MJE33_002144"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBY9648077.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            25512..25829
                     /locus_tag="MJE33_002145"
     CDS             25512..25829
                     /locus_tag="MJE33_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HBY9648078.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
BASE COUNT        10097 a         2802 c         4680 g         8381 t
ORIGIN      
        1 cacttaattt aataatgaaa aaaataataa ttctaaacaa aatcaatttt acataaagtg
       61 ggatttattg gcatagtaat tgcttaatat ataatgtaag agtaaataca attttagggg
      121 tgatggtatg agtcaaagta tggttggtaa gcatgcaatg tggccaaaag aaaatgatgt
      181 tatatttagc atatctggca gagctcaagc agcagagaag gcttttggta tggataatgt
      241 aataaatgca acaataggcg ctctaatgga tgattcagga aaattgataa caatgaaaac
      301 tgtatatgaa gaatataaag cattagataa ctgtgagata ggtgcatatg cagctcttga
      361 aggtcaacca gattacttag aagcagttaa aaaagtattt ttcagagatt acttaccaga
      421 aggacatata agagtattag cttcaccagg aggaagtggt gcaataaaat tagctgtatg
      481 gaactatact aatgaaggag atgaggtatt aacatctgat tggttctgga gtccatatgt
      541 aagtatagct gaagaggcta atagaaaagt agttaattac caactttttg atgaaaatag
      601 aaggtttaat tttgagtcgt ttaaagaaaa atttgtaaat atagcagaaa aacaaggtag
      661 agtctttaca attataaaca caccagcgca taatccaact ggatatagtg ttgcagatga
      721 tgagtgggat aaaatattag acttatcaaa agaagtcgca aaagataaag acaaaaagat
      781 aatatttttc gtagattcag catatattga ctttgcaggt gatgatgatg tatgtagaaa
      841 gttttttaag aaattttcta atttgcctga aaacgttctc gtattggttg gattcagtat
      901 gtcaaaagga tttacagctt atggtatgag aatgggggct ataatttgta tatcttcaag
      961 tgaagatgta gctgaagagt tccattactc atgtgttcat tcatgtagag caaattggtc
     1021 aaattgtaat agaagtgcca tggcagtatt atctaacatt gtaaatgacc ctaaaaaatt
     1081 caaagaatat gaggatgaaa aagaaatata caaaaacatg cttactagaa gagcagatgt
     1141 atttgtaaaa gaagctgaaa gagtaggatt agaaattctt ccatatatag caggtttctt
     1201 tgtaagtata ccatgtgata atccaaaaga agtatgtgaa gaattgacta aacataattt
     1261 atttgcagtt ccattaaaaa tgggtttaag atttgcagtt tgtgcagttt ctgaagataa
     1321 atgtaagaaa gcaccaagta ttataaaaga agcacttgaa agtttagaag taaaaataaa
     1381 taattaattt cattgaacaa attgggtgga ctatgaattt aataattgta gatggaggga
     1441 attttatatg aaacaattga tgacaggtaa tgaagcaatt gcacgtggag cctatgaggc
     1501 tggtgttaaa tatgcatctg catatccagg gactccaagt acagaaatat tagaaaatat
     1561 agcaacatat aaagatgcaa tagttgcaga gtgggctcca aacgaaaagg tagcacttga
     1621 ggctgctata gggggttcaa ttgcaggagc aagaacaatg gcttctatga aacatgttgg
     1681 tttgaatgtt gcagctgatc caatatttac ttatgcatat acaggggtaa atggtggtat
     1741 ggtacttatt acagcagatg agccgggaat gcattcatct caaaatgagc aagacaatag
     1801 gatgtatgca aaatttgcaa agataccttt atttgagcca tcaactagtc aagaagctaa
     1861 agatatgata aaagaagctt ttgaagttag tgaaaaatac gatacaccag ttttatatag
     1921 agttacaaca agactttgtc attcaaaagg attagtggag tgctatgata gagaagaagt
     1981 tgaaattaaa gagtatgtta aaaatgctaa gaaaatggta actgttccag caaatgctca
     2041 aataagaagg ggcgttgttg aagaaagaat ggaaattctt aaaaaattct caaatgaaac
     2101 tgatttaaac tactatgaaa taaatgatac aaaaataggt gttatagcct ctggtatgtg
     2161 ctgtaatttt gccaaagaag tttttggaaa aaatgcatca tatatgaaac taggatttac
     2221 aaatccatta ccttatgaaa aaataaaaga atttgcagaa aaagttgata aaatatatgt
     2281 tatagaagaa aatgacccat ttatagaaga acaaataaaa gcatatggaa tagattgtat
     2341 tggaaaagat gtaataccac catatggaga aatgacacca gatgttttaa gaaaagctat
     2401 atttggaaaa acaaatgata ctatagaata caaatctgaa ttagtaacac caagaccacc
     2461 aagtttctgt gcaggatgtc cacatagagg attcttctat gaacttggaa agagaaaaaa
     2521 tttaatagtt ggtggagata taggttgtta tactcttgga tttgctcctc catataatgg
     2581 aatagattat gttgtttgta tgggttctgc ttttggaact gcacatggag ctcaaaaagt
     2641 tcttaatatg aaggatgata atgaaaaaag attggtaggt gtacttggag actcaacatt
     2701 cttccataca ggaataaatg gacttttaga tgttgtttac aatagaggta attctatttc
     2761 ggttatatta gataatagaa taactggaat gacaggtcac caagaaaacc caggttcagg
     2821 atatacttta caaggtgcta aaactaaaga agttgatata gaaggattag ttaaagcttg
     2881 tggaatagaa catgtaagag ttataaaccc taataattta aaagaagtaa atgaagcact
     2941 agattgggct ttagctatag aagatgaacc atcagtaata ataacaagat ggccatgtgt
     3001 acttaaaaaa ttctctaaag aagatataga agaatttaat aatccattta agactaagtg
     3061 taaagttgac catgataaat gtataggatg taaattatgt ttgaaaactg gatgtccagc
     3121 tctatcattt gataaagaga ataagttatc aaatatagat agaaatcagt gtgtaggatg
     3181 tggagtatgt gcacaagttt gtccaaaaca agcaatagtt aaggaggaaa aataatatga
     3241 ctaagagtat acttttggta ggtgtaggag gtcaaggaac tatacttgct agtaaattat
     3301 taactatggg attgatggaa gctggatatg atgtaaagat gagtgaaata catggaatga
     3361 gtcaaagagg aggttcagtt tcctcacaag ttagatatgg agattgtgtt cattctccag
     3421 ttatagaaat tggtggagca gatatattag tatcttttga aaaaatggaa gctttaagat
     3481 ggttcaatta tttaaaacca gaaggaaaag ctttagtaaa taatcatagg atagattcta
     3541 tgactgtttt aatcggagga gctgaatatc aagaaagtga tatagatgca gagttaagta
     3601 gattaaatgc aaaagttata aatgcagctg ataaagctga agaactagga aattcaaaaa
     3661 taatgaatgt tattttatta ggatgtctag ttaaatctat gcaattagag tctattgatt
     3721 gggaaaaaat tataagtgaa aatgttaaac caaaatttgt tgaattaaac ataaaagctt
     3781 tccatgaagg tatggaaatg gttagcaaat agtttataat agatataaat agactataga
     3841 aattaaggtg aaaggaagaa aattatgact tacagaatat tagccataaa tccaggttct
     3901 acttctacaa aaatagcagt atatgatgga gaagaacaaa ttcttgtgaa gacgatagac
     3961 catccagctg aagagattgc aaaatataat actatacaag accagtttga aatgcgtaag
     4021 gaagcagttt tgaatattct taaagaaaat agtatagact taaaatctct tagtgcaata
     4081 gtaggaagag gtggagtttt accaccagta aaatcaggag catatttagt aaatgaagaa
     4141 atgattgatg tactaagaca tagaccagta cttgaacacg cttccaattt aggtgctgtt
     4201 gtggcacatg caatatcaga acctcttgga atcaactcat atatttatga ttctgttgca
     4261 gtagatgagc ttatagatgt agcgagaata tctggacttt gtggaatgga tagatcaagt
     4321 gcagggcatg cattaaatac tagagcaatg gctttaaaat atgctaagga taaaggaaaa
     4381 gattataaga gcttaaactt aatagtagct cacattggtg gaggagtaag tatttatctt
     4441 catgaaaaag gaagaatggt tgatatgcta tctgatgatg aaggaccatt ttctccagaa
     4501 aggtcaggaa gagtacctgc tacaaaatta gtggctgcct gttattcagg tcaatattca
     4561 gaaagagaaa tgactaaaaa gataagaggt aaaggtggta tagtttcata cctaaatact
     4621 gtagatgcta gagaagttga aaaaatgata gcagaaggaa atgaagaagc aaaaattatt
     4681 tatgaagcaa tggcttatca gttagcaaaa ggtattggag agttagcaac tgtagtagat
     4741 ggaaaggtag atgctataat tataacaggt ggaattgcat attctgaaat gtttacttca
     4801 atggttaaaa agaaagttga gtttatagca ccagtagaaa ttatggcagg agaaaatgag
     4861 ttggaatcac ttgcttttgg aactttaaga gtactaaatg gagaagaaga agctagaatt
     4921 tatagtgaaa attaataaga aaaactaaaa atgtttatat actaaaaaag tggtatatcc
     4981 gttttttata cacgaaagct tagagtatag gtgaaaatgt ataaaatttg gattgccact
     5041 ttttataatt aaaattttat cttaacctaa taaataattc tgtaaaattt cgtatttata
     5101 tatatatttt attggtattt attgttatta ttattatgag gagtgatttt tgatgggaaa
     5161 aataagttta aaaagtatga taagattatt ttcaggtttt tttatatttg caatttcttc
     5221 tgtgcttatg attaatgcac atgtaggtct tatgccttgg gatgtacttc accaaggttt
     5281 atctataaag ctaggcataa ctataggtca ggcttctata atggtaggag ttgtaattgt
     5341 tatattggat gcagtttttg gtgaaaatat aggatggggt actttactca acatgacatt
     5401 tataggaatt tttattgatt tagtaatatt ttctggagtg atacctcatg caagcaacac
     5461 ttatataggt gtgtttatgg ttgttatagg aattattttg gcagctatag caagttttct
     5521 atacttaggt gtttgtttag gaagtggacc gagagatggg cttatgattg ctcttcagaa
     5581 aaaaactaat aaatctgtac gtcttgttag aacaattctt gaaatactag cattagttgt
     5641 tggatggtta ttaggaggtt ctgtaggtat tggtacgctt gtaagtgctt taggtttagg
     5701 atatgtatta caaatagtgt ttagaatatt taaatttgac acaaaattgc tgaaacatag
     5761 atttattata gatgatataa gagaatggaa ggaaaagaaa agcaatgaac ataaatgtaa
     5821 atcaagtatt gtaataaaaa atgagcagaa ttaattatgc taaaatatat ttgtagttta
     5881 tctaatttat agtaaagtca aggagatttt gaaatggaat tatgggattt atataatgct
     5941 gatggaatta aaacaggaaa tgttataaaa agaggtaatt ctattgaaga aggttattac
     6001 catttggcag ttgaagtgtg gattttaaat agtaactctc aaattttaat tcagaaaagg
     6061 tctaaaagta aaaaaacatt gccaaatatg tggggaatga caactggatg tattgtttct
     6121 ggggaagaca gtttagaagg agcaattcgc gaagcaaagg aagaaatagg gatagatata
     6181 actaaagatg agatgaaagt ttttagaagt atgattcatg aagatactct ttgggatgta
     6241 tacttagtta aaaaagaata tgatatatct aaagctattt tacaggagga agaagtaagt
     6301 gatataaagt gggtaagcac agatgaaata agacaattac taaaagaggg tttgtttttt
     6361 gaatatccag agatatacga attgctatat gagatagata ataataaatt aaatatatga
     6421 tgtagagcag atttcagtat aagtaagatt atttaaaatg tatcttctct aaattttaat
     6481 attgttttat gaaaattaat aagttatttt tagtattttt ctacttgttt tttgagatta
     6541 aaataaaaag ggcagtcatt ataaagtcat aaaaatttgg ggaggattta aactatggaa
     6601 gataacaaag gattggcacg tgacagttca aaatataaaa ttgtttctat atctgtcgta
     6661 gtaattattc taattacatt ttggtatatg ttaaatatgg tgcttttaac atttattatg
     6721 acttttattt tctataactt gcttgtagcc acaagaaaac gaattaagaa gttttcttct
     6781 ttaaatattc cagattcatt gataattata gtgttatatg cactatttgc tatattgtta
     6841 gtattgatta gctatgcagt tgtaccaata attatagttc aacttacaga gttgagtagg
     6901 gtttttagtg attttgatgt aaatcaattt gctcaatctt taggaccgaa attgtatcct
     6961 attgtctcaa agctggattt taataaatat atttcacaag cagggctact tatagcatca
     7021 acagcaacaa aagttggaag ttttggagta aatatattat tggcattttt actgagttta
     7081 ctccttttac ttgaaaaaaa tgaaattaaa aattttggag ataaactttc tgatagtaag
     7141 atttctttta tttataattc gcttgtattt tttggaaaga gctttgttaa aaattttggt
     7201 gaagtaatga aagtacaagt tatgattgcc tttataaatt ctgttgtttc tatgattttc
     7261 ttaggtttca tgggattccc tcaaatatgg gctttaggat ttatgatttt tgtgttgggt
     7321 cttattccag tagcaggagt tatagtgtct ttgattccat taactgtaat agcatttaat
     7381 acaggaggaa taacaaaggt ttttggagtt ttacttatga tttgtatagt gcatgcagta
     7441 gaaacataca tattaaaccc taagttgatg tcgaatagaa ctaaacttcc agtttgtttt
     7501 gtttttatta ttttacttgt aggagagcat tatttaggtg tatggggatt actaattgga
     7561 gttccaatat ttatgttcct tatggatatt ctgggtgtta aatttacttc taggtaatat
     7621 attaaaataa taatttattt ttatatataa attatgaatt gttttaaaaa agaacctata
     7681 aatagacatc attaataaag acatctattt gataggttca attttgcttt gtataaatac
     7741 tataaaacta aataaaaaga aatttatttc atttcaaaag tttcacaact tgcatttctt
     7801 tcatttatat gaactttttc agctttgcaa agttcatttt tattgtattt acattttaca
     7861 gcttcacaat gaatgttatc tgtatcaact tggttagttt cattagaacg actagttata
     7921 ccagatgtat atttgtcttc aaaagaagca caataagtat tttctgtagt agttgcttgc
     7981 attccatcta cttttatact accagcataa caatatccac tagtattata agcacaatta
     8041 gtagcagcac aatttaaatt attatttttc ataaataacc tcctaagttt tatttaaact
     8101 tattatgtga agttatctaa attttataca cttatttaca attacattta tcacatttaa
     8161 caactacatc tttggaagtt tcccttccta aatttttatt aacatgatac acatcaacat
     8221 tttcagttga tccacatata gaacatttaa aattgttttc tagtagggtt ctttctttaa
     8281 ataacttcca atacatagaa ttgatgtaat cattataatt taccttgagg catgtctcaa
     8341 gaaagtttat gtatagattg tctaaatctc ctgtatttaa ttctgtatat tctggttcaa
     8401 aatattcaca acatcctcta aaatcgggct ttgtatctga aataacacat ctaatatcat
     8461 atctatagtc taggctaggc cccttaaatg aaagatgtgt gcaaaaagaa caagatattt
     8521 ctggtagatt tttttcaagt tcatgtaatt tattattcat aagtattcct ccaaaaaatt
     8581 tattttaagt atataaatga atagattatt ttaaaacttt tatttattta tattagagat
     8641 ttaggataaa tattccaata aggagtaaat acttaagtat ttattactgt attaaataaa
     8701 aaagaggcaa atctaacttg agattgccta cttttttatc attttttaat aaaatttatt
     8761 ctaagtatta ttttatttag atttagtcag ccaacttagt atctgctttg ctttttaact
     8821 ttctaccaga gcgtacagtt aaaatagcat ataatacagt aaatatggca gctaaaacat
     8881 atgatatatt tatgtttaag ttaaatccaa tttgagcatt aaatatataa ctgaatataa
     8941 caaaggaata aaacatacca ggaattaagc ttataatata attcttttct tttattataa
     9001 ggtaaactgt aatcattgca aatgcaaata tggcaatagt ttgattactc caagagaagt
     9061 atctccacaa tatattaaat ccagaagcac taagctttgc aaatataagg atggctatga
     9121 caggaataaa tatacagatt gacaatataa cacgatgttt cttttccttt tggtcataat
     9181 gaaagtaatc tgcaagcatt aatcttaaag atcttaaagc tgtatctcca gaagttattg
     9241 gtaaaacaat tacacctatt atagctatga taccaccaat agaaccaagt aaatctcttg
     9301 caaccaaacc tattacatca ggagaaccta ctaattcttt aggtattcct ttattgtaaa
     9361 tacccatagc agctgcagcc caaatcattg caattaaacc ttctaaaatc atcatattat
     9421 aaaaagtagt ttttccttct ttttcatttg aaactgatct tgctataaga gtagcttgag
     9481 ttgagtggaa tccagaaaca ataccacaag caactgtaac aaagaatgta ggaataagag
     9541 gaataccatc aggatgaata cctttccaat ttgcaagact taaattagct aaatcataac
     9601 cttgtgtaaa gattccaatt ccaacaccag cagcagataa aagtagaata gctccaaata
     9661 ttggatatac ctttcctatg attttatcaa ttggaaatag tgttgcacac aagtaatata
     9721 gaaagataag tccataaact atccagacaa ctggattatt tatagtcgat ttcatattta
     9781 aaatttgagt aacaactaaa tccccaggtg tataaataaa aactgcacca actaaaagca
     9841 ttaaaagaca aaggaatata ttatagactt gaaatacttt attacctaaa aatctactga
     9901 taagagaagg catttgtgct cctttttctc ttatactaat cattccactc atatagtcat
     9961 gcaaagctcc tccaaataca cagccgatag gtattagtat gaatgctatt ggaccaaaca
    10021 atataccttg gataggtcct agaattggtc ctgtaccagc tatattaagc aactcaatta
    10081 gactattttt ccattttttc attgggacat agtctactcc atcactttga gcaagtgcag
    10141 gtgtttttct gtcatctggg ccaaatactt tttcacaata agcaccatat aaagcacctc
    10201 ctattagtaa tatagccaag ccgattaaga atgttgtcat aatatcaccc catttataaa
    10261 attttttggt ctttgtatac atattatcta aatgtgtaga tttatattga atttaattct
    10321 ggacaaccta caaattctat aaagtattac aactaatata atttcagaaa taaatacata
    10381 tacataacca tgtacttagt atacaggtat ttggtaatat tttcaatgtt atagcaataa
    10441 agaatataaa caaaattctg ataatatttt acaaaaaaat taaaaaatat aaaaattagt
    10501 ttaaatagat ggagaaaaaa ggaaaatcaa ttgagaatat aaatgtctat atcttaacaa
    10561 tattaagagg tttaaaattc ttgaagaaag aatagtcaag tgatataatg ggtttacagg
    10621 tgtatataca ctagtacata tagaaagcgg tgcaattatg gataaagatt taacatatca
    10681 aatcaaagag ctaaagaaag aaaaaaatgc aataatactt gcccattttt atcaaccacc
    10741 agaaatacaa gaattagcag atgcagttgg tgactcatat tatttaagtg agattgctag
    10801 agattgtaaa gaagaagtgg ttgtattttg tggagtaagg tttatgggag aaagtgctaa
    10861 gatattatca cctgaaaaaa ctgtattgat gccagtaagt aacgctgggt gtgctatggc
    10921 agatatggta gatgaagaag gcgtgataaa attaaagcaa cagtatccaa atgctttggt
    10981 tgtatgttat ataaactcta cagctaaagt taaggcacat tgtgatgttt ctgttacatc
    11041 ttctagtgca attaaaatac tagaaaacat agataataaa gaaattatat tccttccaga
    11101 taaaaattta ggaggatata ttgcagaaca atttccagat aaaaacttta tattttggga
    11161 cggatattgt aaataccata acaatataag agctgaagaa ataattgagt taaaagataa
    11221 gtataaaaat gcagaagtat tagtacatcc agaatgtaaa aaagaaataa gagatttagg
    11281 agattatgta ggtagtacat caggaataat aaaatatgct actaatagta aaaacaaaga
    11341 ctttataata gcaactgaag aaggtatatt acatgaatta aagaaaaata atcctaataa
    11401 aaacttttat attcctggtg gaaaaatact atgtacagat atgaaaaaga caacattaga
    11461 aaatttatat agtacactta aaaatatgga aaatgaagtt attgtagaag atgaaatcat
    11521 ggaaaaggct ttaaattcac tgcttaacat gcacaaatta gcagaaggat agagggatag
    11581 acttatgaat ttagaacaag atgttttaat agttggaagt ggagtttcag gattatactg
    11641 tgcattaaat ttagataaaa gtttaaatgt gctagtagta tcgaaatcaa caattgaaaa
    11701 caacaataca tatcttgcac aaggtggaat ttcaactgct agaaatattg atgatataga
    11761 atcctttgta gaagatacaa tgaaggctgg gcagtataaa aatagagttg aagcagttca
    11821 agtacttgca gatgaatcta tagaaaatgt aggtcagata gtagaatatg gtatgccttt
    11881 agacaaagaa aatggagaga tagattacac aagagaagga gcacatagtg taaatcgaat
    11941 agttcatagt aaggataata ctggagaagt tgtttttaaa actttattaa aagaagctaa
    12001 aactagagaa aatataactc ttatagaaga tgcatatctt ttagatatat taaaagatgg
    12061 caataaatgt ataggagcta gaatttttaa atctaaaaaa gagattcatg ttttttcaaa
    12121 aatagtagtt ttagctacag gtggaattgg tgggcttttt aaaaattcta ctaatcaaag
    12181 acatcttaca ggtgatggaa ttgcaatagc tttaagaaat aatataaaaa tagaaaattt
    12241 agaatatatt caaatacatc ctacagcctt ttatgaagaa aataatgaag gtaggagaat
    12301 gcttatatct gaatcattaa gaggagaggg agctaaactt ttaaataaaa ataaagaaag
    12361 atttgtggat gaacttcttc ctagagatgt agtttcaaaa gctatttttg agcaaatgga
    12421 aaaagataaa ttaccttatg tgtatttaga tgcaacacat ttagatagtg aatatttaat
    12481 taatagattt tcttttattt ataatgaatg tttagctaga ggaacagaca taacgaaaga
    12541 atgtataaag gtatctcctg cacaacatta ttttatgggc gggatacatg tagatttgga
    12601 ttcaaaaaca tctatggaaa atttatatgc agttggtgaa attagttgta caggtgttca
    12661 tggagccaat agattggcaa gtaattcatt acttgaaggg cttgtatttt caaaaagagc
    12721 agctaaaaat ataaactcag ttatagataa tgtaaaagtt aagtttatag atgtaccaga
    12781 tatggatata gatatagaac aggtaaaaaa agaaaataaa atattagtca taaaagaaat
    12841 tgaaagaaca agtgaggatt ttggcgatga attatttgat tattgataga atgatacagg
    12901 atgctttgat agaagatgtt ccaagtgaag atataacaac taattctatt gtggatgaaa
    12961 attctaaatc tactgtagat ttaatttgca aacaagatgg tataatagct ggcttaggtg
    13021 tatttaagag agtttttgag atacttggtg atgtagatgt aaaattatat aaaaatgatg
    13081 gtgatagagt aaagaatcgt gaaaagatag ctgtattaac aggtagtact agaaacttac
    13141 ttgtaggaga gcgtgtagca ttgaattatt tacagagaat gagtggtata gctactatta
    13201 caagtaggta tgttgaaaag cttgaaggta caaatacaaa attattagat agtagaaaaa
    13261 cgatacctaa tcttagaata ttagataagt attctgtcaa agtaggtgga ggatgcaatc
    13321 atagatttaa tttatctgat ggaatattgc ttaaagacaa ccatattggg gctgctggta
    13381 gtgttaagaa ggcagttgaa ttagctagaa aaaatacttc ttttgttaga aagatagaag
    13441 tggaagtgga aactttagac atggtaaaag aagcaataga agccaatgca gatataataa
    13501 tgttggataa tatgagtttg gaaatggcaa aacaagcagt agatattata aatggaagag
    13561 ccattgtaga attttccgga aatgtaaatt tagatactat agaagatata ggaaaaattg
    13621 gtgtggatgt tgtttctgta ggagcgctta cacattctgt taaagctctc gatataagta
    13681 tgaagaattt gagaaatata taaattttat attgaaataa acttaattaa aatagactcc
    13741 ttaaatttgt tttataaatt taaggagtct attttttatt cattaaaatt aataaaaata
    13801 gaaaaaaatc aatcaataat ttaataaaaa tagataaata ctatatacac ttttaataat
    13861 atagttaata tctattttac agataaataa gaataaactt ataatataat tgtatctgta
    13921 aaattttgta aaatataaaa agtatagggg gaataaaaat gaaagtagct tatattttta
    13981 gtagtactaa tacacataaa attctagaca aaatgataat accacaactt gaacaaggaa
    14041 ctcatggagt agacgttcta gggatgtttt tctttatgga taatacctta tttttatcta
    14101 aaggtaatcc tgtaggcgag agactaagta aattacatga aaaaactgat atgataataa
    14161 tggcttgtga ccaatgtgct atagaaagag gtatagagaa taatttggtt gatggagcta
    14221 caattggatg tttcccaaac ctatatgctt gtttaggcag tgcaggtgtg gaccaagtta
    14281 taacgttata aattagtata ggatacaatc catagaaaaa tttattttct gtggattatt
    14341 ttaagtaaaa gataaattgt ttaaagtagg ggatgaacaa atgtctaaaa ttttagttga
    14401 atttataaac acttgaccat cttgtgatga acatggagtg tttataaaaa aaatgtcaga
    14461 aaagtatgct gataaattgg aaataaaatt atatcaagca gggaaagatt tttcgtatat
    14521 aaaaaaatat gggattataa caaaaggaac tttaataata aatcagaaaa agaaatatga
    14581 tagattaaat aaagatacaa ttgaaagggc aatagtagaa gctataaaca ataattaaaa
    14641 gtttaaaaat taggagtatc aaaaatgaat atattagaaa ttgttttaaa tggagttaaa
    14701 gatactatag tagcttctat agttacactg aatggagcat caggatggct tatgataagt
    14761 tttatcttgg caggtgtact tcacaatgtt ttaactccaa gaaggtttca aaaacaactt
    14821 ggaaataaga aattttcttc tttaattaaa agtactatct caggaatgtt tttaccaata
    14881 tgtagttgtg gagtaatacc acttggaata agtatgtatt attcaggagc atatttagga
    14941 ccagtacttg cttttatgac atcaactcca ataataaatc caatagctgt agttctttgt
    15001 ctaggtcttt taggtcctaa agttacaatc atttatctaa tagctggttt tttagtacca
    15061 tttttagtag gatttttagg aaattgtttt ggaaaagaag aattatcact tcataatgat
    15121 tcagaggaag aagtaataga attagaagaa gaaaaaagaa gttttatgga aaatatgatt
    15181 gatggtctta aatggtcttt tggtgaatta gcccttacta ttagtaaata tgtagtaatg
    15241 ggtatggttt ttgcaggatt tataacaact atttttccaa atagtataat tcaaaaatat
    15301 ttaggaaatc caggtttgct atctcttgga agtatagcaa tacttgcatg tgttatgtat
    15361 gtatgtgctg taggacatat accgtttata gcagcccttg ttgcaagtgg agcatctcct
    15421 ggtatagcga taacatttct aatagctgga gcagctacaa atctaccaga acttataagt
    15481 atgtttaaac ttattggaaa aagaactgtt attatatatt ctgtaacttt gacactgtct
    15541 tcaataataa ttggatatat aacaaattta cttttagtga ataataactc agtaaattta
    15601 gatgcaacaa attcttcaat acagttggca aataaactaa tgttaaatat acctgatagt
    15661 tttgagtatg tatgtactat tttaattatt ttatttgcat ttaaatcaat aataccaaag
    15721 atgaaagagg tcataggaaa tcatgcttaa aaagatagtt ataatatcct gcatagtagt
    15781 tgtgttaatc attttatcaa aaatagttga tgataacatc aaagaagatg cttctattcc
    15841 aaatgtgaat aaagaaacat tagaatattt tagaaaaaat tataaagaag acataataac
    15901 ttgtgctgaa gaagacttaa ataatgatgg gaaaaaagat ttagtagtta tatataaaaa
    15961 aagcaataat tcaaatgaaa tggtagtcgt agtttctgat aaaaatagcc actatataac
    16021 aaagccaata ccagcaccta tagagaatca aactataacc tttaaaaata ttgatgataa
    16081 agcaccaata gaagtaattg tatcagggtc taaaaatggt aatgtaggat atgctattta
    16141 cagagtagag ggtaaaaaat ttgttgattt atttggcgaa gatatggaca aatgttgcta
    16201 aaaataaaaa taatacataa aggtgggaaa tttatatgaa attatctaag aagattcaag
    16261 cattaatctt atttggtgta atgggtacat ctatattaac aggatgtagt agtaaaccaa
    16321 aagaaaaaga agaagcaaaa actggaaaag atgattatac aataacagta ggttattata
    16381 attgtgacca tatgactgcg ggaccagtag cagaatcggc aggaatatat aaagatttag
    16441 gtcttaatgt aaaaactgtt ggaaatggta aagttcctga agctatggca gcaggaaaaa
    16501 tggatgcagg ttacatagga actaaggggt tagttggagc aatacctaag ggttcaccaa
    16561 taacaatagc agctaacaac catactggtg gttctgaata tttaatagtt tcaaaagata
    16621 taaaagaacc taaagattta ataggtaaaa aaattgcaac agatatgagt gatttcttat
    16681 ggacaagtga ttatggtcca gaaacaggtc taccaactga cccatcaaaa tatgaagttg
    16741 taaatatgga ttcagacaaa gataaatatt tagctcttaa aactgggaaa attcaagctt
    16801 ttacatcttg tgacccttgg ggttctgtag ctgaaaatga cggtgcagga aaaataatag
    16861 catctactca atataaagaa aaagctaatg gcaaagaata taactgttgt tcattctcat
    16921 taaacaaaaa ctttataaaa gaacatccag atttagcaaa aaaattggtt ctagcacata
    16981 caaaagcaat agaatatata tatacaaatc cagctgaagc agctaaaata tttgcaaaat
    17041 attataatgt agaagaagaa gttgcactta gaactatcta taagaagact gttggagaag
    17101 gtagaactct tacttggaaa gttacaggtg aagaatataa aaataattta caaatgtata
    17161 aagatttaaa ggctttagat gatataccta aatatgaaga tacgattgat acaagcttat
    17221 tggactcttg tggagctgat gattttgaca aatttataaa agaaaaagtt aacccagagt
    17281 ttcctgaagg tatgagttat gaagactgga aagcaaaggt attataaata aatggagaat
    17341 atgaaaaata taataatact ttttatcatt attttgggaa gtatatttat aatatttttt
    17401 aattttgaag ataaagaaga aataaaagga aaattaagaa taggggtatc tgatgatacc
    17461 tctggttttg taataaatta tatggtaaat agagactact ttgaaaatat aaaagtaagt
    17521 gatataatgg atatttttac aattaatgat tgctgagcaa gtacaatgca gtgggctctg
    17581 agttcagagg aaatagatat agcaatcata tgtaaagatg ctgcaaaaca atatgtaaat
    17641 gtaaatagtg gatttgaaat tgttggtact gtagttcaaa attctgatat atttctatta
    17701 agtgataata atcctgaaaa gataggagtc attcaaaata gagattacca gtcagaattg
    17761 gttaaaaaat actacaagaa tgtggaagta gctcccttat tagggactgc actagcatat
    17821 ggattagaaa gtaatttagt agatggtgtt gtaatagatt gcataaagtc aattgggtta
    17881 gagggcaaaa gaaaatctac aactaaatta ggagattatg atacctatgt attagttgta
    17941 agtaaagagt ttaaaaaaag taaagcatat agtgatttta taacacttta caataaaagt
    18001 gttgatgctt taaagaataa agctacattt aagaaagcat taaaagagta caggaatata
    18061 agaaatatag atatggagga aataagcaat tggaaattaa aatttctaaa gataaaagcg
    18121 atagattaac taagaaaaaa actgatggta gtatggatat tttatataaa ataatactat
    18181 tagttattat atttttagtc tggcattttg cagcaaaaga tatagggagt tctttgttac
    18241 ttccaatgcc agtagatgta ataaaaggtt tctttttctg tgtgacagat gcagagacag
    18301 tcactaatct ttttataaca ttgcagagag tacttaaagg ttttatgtat gcattattgt
    18361 ttgggcttcc aataggattt attatgggat tttctaaaac ttttgaaagg gttttatctc
    18421 cagtagtaga ttctgtaagg caagttccaa taatggcatg ggttcctctt actatagtat
    18481 ggtttggtat aggagatggt ccaacaatat ttttaatagc tttttcaggg gttttcccta
    18541 taattttaaa tactattcaa ggagtaagag caatatcaaa ggattattat aatgcagcta
    18601 gaagtatggg agcatcgcca attgtgatat ttacaaatgt aattgttccc gcatctcttc
    18661 cagatatact aactggttca aggattgcta taagtacagg atggatgtct gttatctgag
    18721 ccgagtttat agctacgagt gccggtctcg gttactcaat ggtacaagct caaacattaa
    18781 tgcatacaga tgtattaatt gggcttatga tatttgcagc tttaatagga tttataatag
    18841 atagggtatt aaagtttata aataaaatcc tttgtagatg gaggtttgca gattaatgaa
    18901 gttaagtgta agagatataa ataagacatt tgtaaataat agagtacata caaaggtttt
    18961 agaagatata agtatggata tagatgatgg acaatttgtc tgtcttttag gaccatcagg
    19021 ttgtggaaaa acaactttac ttactataat aggtgggttt caaaaatcag aacgtggaga
    19081 tgtttttata aatgataaaa gagttaaaaa accgggcata gatagagctt ttatatttca
    19141 aaattatgct ttatttcctt ggaaaactat aagaggtaat gttctatttc ctatgaaaca
    19201 gcagaaaata ccaaaagaaa aaagagaaga aatgctagaa gaacttttgg ttatgtcaga
    19261 cttgaaagga aaagaaaaac tgtttccgca tcaattatct ggaggaatga aacaaagagt
    19321 ggccatgata agagctttag catgtaatcc agaggtatta ctaatggatg aaccattagg
    19381 agctgtagat tttcaaatga gacaaaattt acaggaagag ctagaacgaa tatggataaa
    19441 gaagaaaata actgcattaa tggtaactca tgatgttgat gaagctgtat atatgagtga
    19501 tagagttata gtaatgtcga gagataaagg aagaattatt gatgatataa atatagatat
    19561 tccaagacct agaattagag gaagccaaaa atatgaagag tacaaaaaca aattaactga
    19621 tactttatca aagtgttatg aagtttaaag aggagtagac atgagtcaaa tagaaaaata
    19681 caatagtttg gataatacac aacagaaaat aagaatctct attatggata tgataataga
    19741 taaaggttct agtgttacac ttcaagaagt gactgaatat ataagtaaaa agttgaatat
    19801 agaaaaagaa tatatagaaa ggacactaca atattttatt tataaaaata taatggttgt
    19861 tgatgggaat agtataaatt ttatatatcc tgtatcagca ttgccaacaa atcataaagt
    19921 aactttgaga gataatagaa gtttcagtgc aatgtgtgct atagatgcta taggaacctc
    19981 ttgcacattc aaccaagata taaaaataaa ctctatttgt agtgctacag gtaaggaaat
    20041 agaagtaatt ataaaaaatg aaaagattga gtatgtgaat aatccaaact taagagtgct
    20101 tcatataaat cttgataagc atctaaattg ggctgctagt tgctgaaata taatgaattt
    20161 cttctggacg aagagtgatt ttgatgcttg gacaaacgaa gcgggattat ctaatgatga
    20221 agatatatat tgtttagata ttaatgaggc aatagtagaa tcatataaaa tatttaaact
    20281 caaacaaaag gtactatcat agatatacat atatgaaaaa ctaaaaatga cctttagata
    20341 aaaatgatag gtaatatcca tactatataa attagtatag agtagggatg tgaaaagtat
    20401 ttaaatctaa aggtatattt ttagtttttt ttagatgatt agatagcctt attaagtata
    20461 tatttattta taacctttgc aactccattt tcattattag tatcagtaac ataatctgca
    20521 atttccttta tactattacg tgcattcccc atagccacac caagacctgc atactttatc
    20581 atatgttggt cattttcttc atcgccaaca gcaatagcat tttctatagg tatatttaga
    20641 ttcgtacata atgttttaag agcagttcct ttattagtag ttttacctaa aaattctaga
    20701 taaaaaggag cagaccttac tatagtatat ttattgaata aatttttagg tagttgctgt
    20761 atgatttctt ccaatctttc tggttcatca actatcataa ttttacagat tttaatagat
    20821 tcatcaattt tatctttttg tatatatttg acttctatat tatttaattt cgcttcatga
    20881 agtgtatatt tgccaggcgt ttcatttggt gtaattaaat ttgtcattgt atttatgtgt
    20941 attttagtct tatatttttt acaaacgtta ttatatataa aatcaaaatc atctaaactc
    21001 atttctacat catgtatgat tttctcactt ttaacttctt gaataattgc tccattaaag
    21061 catataacat aatcaccttc atctgttaaa tttagttgat ttagaagttc ttttacacca
    21121 ggaagaggtc tgcctgttgt tataactact tttgccccag ccctttttgc ctcctgaatg
    21181 gactcaaaga cttcaggagt aatccttttt tgtgtattta atattgttcc atcaatgtct
    21241 agtgcgatta atttgtacat atcaacctcc aataaattat atactcaaat ttcctgtgaa
    21301 aacgttgaca cagctagtgt tgaacaaagt ttcagtggga aatatggttt gttttgtata
    21361 ctattatatc aagagataag taatttagaa agtttaataa tattaatttt ttgataaaaa
    21421 tattacttat gtgagatgag aatgtaaaaa ctttgtaatc aataattcca aaattcttaa
    21481 ttacaaataa tggtatcgtt tgcattgtta atataaataa atgtatatta tatagttaga
    21541 agtttgaata aatagactga tggtaatttg ttatattagt tagttttgtg ttataaaata
    21601 ttgtaattcc attaatcata tgttattatt caacatgatt ataatataaa gaatatttga
    21661 tatatataat aatttataaa agagtattac atagtacatg taaatctata tttataaata
    21721 caataaagta aagttgtaaa ggagtgcttt aaatgaagtt ttcagagttt aagtatgaaa
    21781 gacccaatta taatagtatg aaaaaagagt ttttgagttg tgtagaagat ataaataatt
    21841 ctcgtaatta taaagagcaa caaaaaaata tacataagat taatttatta agaaataaaa
    21901 tagaaacgtt gtcaaatata gcttcgataa gatatagtac agacacattt aataaattct
    21961 acaaagaaga aaaaaattat tgggatgaat atatgccttt gtatgaagaa ttaaattcat
    22021 atttttataa tgccattgta aattcaaaat ttaaatatga tttgataaaa gagtttggag
    22081 agcaattttt cacaatagta gagtattctt taaaaagttt ttccaaagaa ataataagtg
    22141 aattgcagga agaaaataaa ttgtgttcag agtatactag acttttagct tcagctgaga
    22201 taatgtttga tgggaaaata agaaatttat ctggtatggg gaaattcatg tattctaaga
    22261 atagaaaaac aagagaatta gcaaacaaag catattataa tttctttgaa gaaaatgaaa
    22321 ctaaatttga tgacatattt gataaattgg ttaaacttag agataagatg tctaaaaaat
    22381 taggttttga agattttgta gagcttgggt atgttagaat gatgagaagt gactacagag
    22441 aatatatgat aaaaaatgtt agaaaacaag tactaaaata tgttgttcca atggcaaatg
    22501 agctttatga aaaacaagct aaaagaattg gtctagaata tttaagttac atagatgaag
    22561 gcgtggaatt tttaacagga aatgcaagtt taaagggaga ttcaagatac ataattaaaa
    22621 atggtaaaag gatgtattct gaactgtcaa aagaaacaaa tgaatttttt gattttatgt
    22681 tagaaaatga acttatggat ttagaaacga agaaaggaaa aggagctgga gggtattgta
    22741 cttatatacc agattataag tcaccattca ttttttctaa ttttaatcaa actgctgatg
    22801 atatagatgt attaacacat gaggcaggac atgcatttca gttgtatatg tctagatgga
    22861 ttgatatgcc agaaattaat tttccaacgc ttgatagttg tgaaatccat tcaatgagta
    22921 tggaatttat aacatggccg tggatggatt tattttttaa agaagataca gataagtaca
    22981 aattcactca cttatcatct tcaattaaat ttatacctta tggagtaatt gttgatgaat
    23041 ttcaacacta tatttacaaa aatcctaatg tagataagtc aaaacgaaaa gaaatctgga
    23101 gatttttaga gaaaaaatac ttacctcata gaaaatatgg tgataattct tttttagaga
    23161 gaggttgttg gtggtttaaa caaggacata tatttaaaaa tccgttttat tatatagatt
    23221 atgtattagc tcaaatttgt gcgttacaat tttggaaaaa aatgattcaa gatagagatg
    23281 caggatggaa agattatata aatatctgta aagttggagg aacaaaatca tttttagata
    23341 ttgtgagtat gggaaatttg tattctccat ttgatgatgg ctgtattgag tctattatag
    23401 gtgatgttaa atcttggttt gatgaaataa atgacagcaa attataaaaa attatgagaa
    23461 ttttattgta taaaagtgaa aaattaaaaa atatataaaa agtgattatt tgttgtgaaa
    23521 atagtcttgt ttttaactaa attgataaaa taaactaaaa aagatttgta cttaaaaaaa
    23581 aaagaaaata caagaaatat gcaatatcaa catttggaaa aaaaagtaaa aatgtataca
    23641 aaatacatat aaaaaaacta ttgcaaattt tatagaatgt ttatatactt aagtcaacag
    23701 agatagaaaa aagctattta tagaaaacaa aggataaatt gcttaatttt aaattttctg
    23761 aaaaaaataa taatccggat gaagagtatg ggagactgct ttataaaaag cgaccgaagg
    23821 gacaagttta atcttactta cccaaaagat tagatgaaat tctcaggtaa aggtgactgt
    23881 acttggacgg ggctctgaaa atgattttaa cagggaagac aaattgggag atttgttttt
    23941 cctgtttttt atttatcaag taataaataa aagtttgaaa gagagtaaaa aaatgataat
    24001 aattacgaat aatccaaaag taaaagaaga agttcaaggc agagaagttt tgtttaaaga
    24061 cacaacttat attggaatct tagaagcaag tagagatttg atacatgaag gttatgaact
    24121 tttatctcat ccactctatg ggagtgtaaa gccaaatgaa acaccttaca ggactgttat
    24181 actaaagaaa ggaaatcgtt tggatataaa ctctttaacc ttaattgaag aagcaatcat
    24241 cactgcaagt aaatttcaga ataataaaaa gactccaaaa tggacagaaa gtgttcaaga
    24301 tgattttaga gtaattgatt atgatatatt ttataataca atacaaagaa tgcagtatga
    24361 ataaaaaaaa attgttaaaa agttattaaa acagcctaaa tattaaatct aatttaatat
    24421 taaataaaat gagaaattta attggggagg ataagtcaaa atggaaaatg tgtatgactt
    24481 agtaataatt ggttcaggac cagcagggct tgcagcaggt ctttatggag caagagcaaa
    24541 gttaaaaact ctaatcctag aaaaagacaa gactggtgga caaattgtaa taacacatga
    24601 aattgcaaat tatccagggt cagttccaaa tgcaacagga ccaagtttaa tagctagaat
    24661 ggttgaacag tgtaaagagt ttggagcaga aatgctaaga gacaacattg ttgatactga
    24721 gctagatgga gatataaaag ttttaaaagg ggaaaaggca gaatatagag caaaagcagt
    24781 aataatagga acaggagcaa caccaagaaa gattggttgt cctggagaaa aagaacttac
    24841 tggtaaaggg gtttcatatt gtgcaacttg tgatgcagat ttctttgaag attttgaagt
    24901 atttgttgta ggtggaggag acagtgcatt agaagaagca atgtatttaa ctaaatttgc
    24961 tagaaaagta acaattgtac atagaagaca aggatttaga tgtgctaaga gtgttgaaga
    25021 aaaagcaaaa gctaatccaa agatagagtt tttattagat acagtaattg aagaaatcaa
    25081 aggtgatgga atattagaat cagttgtatt taaaaataaa gttactggag aaactcatga
    25141 atactttgca gatgaagaag atggaacaat gggagtattt gtgttcgttg gattagatgc
    25201 tcaaactgat ttattcaaag gtaaagttga tatggatgaa aaaggttaca taataactga
    25261 tgaagatatg agaactaaca taccaggcgt atttgcagca ggagattgta gatctaagac
    25321 tctaagacag gttgtaactg caactaatga tggagcaatt gcatctatag tagctgagaa
    25381 atacattgat gaaaaatttg ggaactagaa tctgtaaatt aagaaatagt gtctgtaaat
    25441 ttaatgaata atatttaaaa ttaaaattaa tatttaaaat aaaaataaaa atttcaaggg
    25501 ggaataagaa aatgttagat ttagataagg ctacatttga agaagaagtt ttaaatgcag
    25561 aaggttttgt atttgttgat ttttggagtg aaggttgtga gccttgtaaa gctttaatgc
    25621 cagatgttca taaattagct gagacttatg gggataaaat aaaattctgt aagatggata
    25681 caacaaaagc tagaagatta gctataaaac aaaaagtatt aggtcttcca actatggcta
    25741 tatacaaaga tggagaaaaa gtagacgaag ttactaagga tgatgctaca gttcctaata
    25801 tagaaaacat gattaaaaaa tatctttaat atgttgataa attattaaca ttaaaagaaa
    25861 aaatctattt ttatctgaag ataagaagaa aaaggttttt atgtccaact ttgttaaaaa
    25921 aataatttta aataaaataa tttttttaca ataaatattt
//
LOCUS       DAGSRM010000047        25671 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.047, whole genome shotgun sequence.
ACCESSION   DAGSRM010000047 DAGSRM010000000
VERSION     DAGSRM010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25671
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.047"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..950)
                     /locus_tag="MJE33_002146"
     CDS             complement(<1..950)
                     /locus_tag="MJE33_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBY9648079.1"
                     /translation="MKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVMYDEKTKNIDKF
                     KSLIRPVSNEELYPHIQELTHISSEELKSAPSYEEVMRKFKKWLGIFSDIKGIYTFGN
                     LDLTCFNNTDMRSAKKNNHPRFVNNIRDLFVDIKEKYINCGMRCMNYISLKNLLEFVN
                     LEFSGDAHDPLNDAYNLFILDEAITNNVDIQNLLIIRDIIRPPFNDINSNLDNCFNKF
                     KESLYKKEGNYNIVDFSVEIIKTVRMYLLTIIDVNIQNLEIMKDISKKMDTIDKLKDI
                     EEGYFYLLEDVYFDMKDVLEDLMLYRMNEDEYKYEIKNII"
     gene            complement(998..1858)
                     /locus_tag="MJE33_002147"
     CDS             complement(998..1858)
                     /locus_tag="MJE33_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBY9648080.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKAI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLESNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            2127..2336
                     /locus_tag="MJE33_002148"
     CDS             2127..2336
                     /locus_tag="MJE33_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBY9648081.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            2336..2602
                     /locus_tag="MJE33_002149"
     CDS             2336..2602
                     /locus_tag="MJE33_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBY9648082.1"
                     /translation="MKDSSRAELMRKVQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            2631..3203
                     /locus_tag="MJE33_002150"
     CDS             2631..3203
                     /locus_tag="MJE33_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBY9648083.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(3438..4628)
                     /locus_tag="MJE33_002151"
     CDS             complement(3438..4628)
                     /locus_tag="MJE33_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBY9648084.1"
                     /translation="MNFNQKRIAASIMATAIIMPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKEGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTNENSENSSNDLVKGTKVVTAKSLNVRTGPGTSHSKIATL
                     SYGTEVGSISESGGWTKISYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGADSVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSVGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLVFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSSKKVTISNINSSYYSSRYVNA
                     RRVL"
     gene            complement(4888..5319)
                     /gene="dut"
                     /locus_tag="MJE33_002152"
     CDS             complement(4888..5319)
                     /gene="dut"
                     /locus_tag="MJE33_002152"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBY9648085.1"
                     /translation="MYNLKVKKLNDDAIIPNFAHKGDAGMDLYSIEEVVIPPGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIVKPIYDINIEEVKELSDSERGKGGFGSTGF"
     gene            5432..7660
                     /locus_tag="MJE33_002153"
     CDS             5432..7660
                     /locus_tag="MJE33_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HBY9648086.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEDEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAEAGEYKGKYVKGKESKFDTLEDANKIIASITSETGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDA
                     IKDRFVANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVDVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKISQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNT
                     EKEVAISKDSNNKKEVKNGDENQSLGKCPICQGDVLEFDKGFACKNHKECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDATLKYDKNPDTGYFNWKIEF
                     DN"
     gene            complement(7771..8256)
                     /locus_tag="MJE33_002154"
     CDS             complement(7771..8256)
                     /locus_tag="MJE33_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBY9648087.1"
                     /translation="MDNNNILKIADSFLDFLFVLENNIFKESDLLKKFQNNSDIMKAY
                     FEECPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDP
                     KDRRILRVELTDKAFKLFDAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKL
                     G"
     gene            8508..9731
                     /locus_tag="MJE33_002155"
     CDS             8508..9731
                     /locus_tag="MJE33_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBY9648088.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTEESEPEKPIAVSVQKAIGGEIE
                     NTNSFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPELENSVKQSK
                     ASLDLAKASYSSATGGSLEAQVNQAKTALDNAKIQYEESQRNYDNNKILYEQEVISLD
                     QFKKIEFSLEQTKQQLDSAQRAYDTATSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTSPVDGTITAKNFDSKEMITQSQPAFIISNPNILEVDLNVAESDIGKFKKDGNVD
                     VIIEDQRILGKIDYVPSVVDPQTSLYPVKVLVNNTNNKFKAGMSAQVNLSVEKENGAV
                     TVPKKAIFEENGKKYVYIATKDNIAKKHLVQTGIVTEDKIEIKSGVSDKDTVIIGGIS
                     LISDGTKIFPVEKEK"
     gene            9735..12869
                     /locus_tag="MJE33_002156"
     CDS             9735..12869
                     /locus_tag="MJE33_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HBY9648089.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQVSQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGEKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     SNLLISAVIAFIVIFAFLKSVRASLVVAVAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSLDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTEGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSRGKNSQKMQEKDSPIFDK
                     VSEAYKKLLSVCLRHQRLVVISSLLIFVASLFGMGFIGMDFMPAADKGELSISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNSKASIAIELESQKNRKRTTDE
                     IEKEIIDRVATVPDCKINVSQNSSVMGGAGGADFTLEMKGPDLDVLKILGKEAEKEFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGVNTAEIAGMLRTAINGDSVTTATIDD
                     YKVDVNLKFKADTIDSLEDIKQLKVNSATGKEVPLGAFSDIKMADGLQSISRTDGDFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGETEMMNESMASLGFSMVIAI
                     ALVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTVSTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGSI
                     KRKIIKKPKLKAKV"
     gene            12903..14201
                     /locus_tag="MJE33_002157"
     CDS             12903..14201
                     /locus_tag="MJE33_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HBY9648090.1"
                     /translation="MSKIVAIGCGVGIITANLIPVYADNNTSSINKVDNSILSDSNKR
                     TSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEFDSFTGSDNSFPYDQI
                     GLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMKLKKD
                     LGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEYRFES
                     FRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVAPTKP
                     EQGNLTDDEYKVLMDKYEKDYDRYKKEVEAYNLERQTYAAGLTTYANYLQQKFNTENG
                     LVTLEDSKKALKKGLIDSYAQLLALEDTIQITKKQLDLSEKQLKNTKLRYDLGLITLT
                     DYKKQVVSNEDAKNSYDSLIVNYNSLKNGIEKPWILNTGK"
     gene            complement(14490..14735)
                     /locus_tag="MJE33_002158"
     CDS             complement(14490..14735)
                     /locus_tag="MJE33_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBY9648091.1"
                     /translation="MTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCPEPSCRALV
                     SRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKK"
     gene            complement(14949..17576)
                     /locus_tag="MJE33_002159"
     CDS             complement(14949..17576)
                     /locus_tag="MJE33_002159"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBY9648092.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRAEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRV
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDKALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GDVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGIEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            complement(17637..18479)
                     /locus_tag="MJE33_002160"
     CDS             complement(17637..18479)
                     /locus_tag="MJE33_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBY9648093.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGVLQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            complement(18486..19124)
                     /locus_tag="MJE33_002161"
     CDS             complement(18486..19124)
                     /locus_tag="MJE33_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY9648094.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
     gene            complement(19560..20852)
                     /locus_tag="MJE33_002162"
     CDS             complement(19560..20852)
                     /locus_tag="MJE33_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBY9648095.1"
                     /translation="MLDLNRKLAKLEEEGKKIKVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDINLELAHKAFTDAGIDEEIIDNVTNEKDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAERKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLDHKA
                     TIVPKAGLVAEVMTIAKKDLKKGEFMDGYGAYTCYGTIEKYDVAKAMNAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
     gene            complement(20889..21245)
                     /locus_tag="MJE33_002163"
     CDS             complement(20889..21245)
                     /locus_tag="MJE33_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBY9648096.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     IATLKSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKCEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            complement(21280..21639)
                     /locus_tag="MJE33_002164"
     CDS             complement(21280..21639)
                     /locus_tag="MJE33_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648097.1"
                     /translation="MKLLVILFVAYILNTIFIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAIISSDSDGFIKFGEILKGRTVMAKFKEIEEIEGLDIYSAEQKFKDEES
                     IVQAIKFIKEKINVSFN"
     gene            complement(21708..23681)
                     /locus_tag="MJE33_002165"
     CDS             complement(21708..23681)
                     /locus_tag="MJE33_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBY9648098.1"
                     /translation="MSVTEKIPSIDRKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAVIKYIKGQGYKLEIKDEQILNKIIDMNRKNVFSLNSKEDRVEF
                     ILNYLIELDGFITLDSLADEMCVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDLSNSKYFKGIKKEEIINLEEKLLTLFKKNNFYVTDEML
                     REVINYIVVLVYRVKEEKKVKDYDVKFDLLKSYDEYFIAREIKHIISGMFECTLNDEE
                     IIYLTIPLVSGNAPASECALNSSRISKNIDELMENIFNQIYVDMGIFINEDELRVGLG
                     YHLSFTLNRLLFNIKLKNVLLEEIKQNCILPFKLAQIAGKVIEKKYNLEVSEDEIGYI
                     AIHFSGYLERNSSRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLR
                     NINLDEYDIVFTTIDLDTSNINTIVLKINTIFDENKLREQLKTVLCLREGNIDTTNST
                     NLLINNLLDEDKFMILNEKTILASLEKMMDHLMDLGCIDEKFRKNIVNREEKSPTVFD
                     KGLLFPHSINEKSDKFLMAVGILEEPIEYANRSIKIILMTMFPCENKIDSDLLVKIYE
                     EVLKIGQDVKLTNKISKCRTFLEFKKVLLKNLI"
     gene            complement(23903..24916)
                     /locus_tag="MJE33_002166"
     CDS             complement(23903..24916)
                     /locus_tag="MJE33_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBY9648099.1"
                     /translation="MEENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEIVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVNPNCISVVDGEDMPQKSQENKSENKSSIRKPDNYDEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     ARVISPLAGNIGGLLIISVICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPIAVIIAYGVAVF
                     ALR"
     gene            complement(24929..25474)
                     /locus_tag="MJE33_002167"
     CDS             complement(24929..25474)
                     /locus_tag="MJE33_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBY9648100.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNRATNKA"
BASE COUNT         8786 a         4267 c         3269 g         9349 t
ORIGIN      
        1 tttattatat tttttatctc atacttgtac tcatcttcat tcattctata aagcattaaa
       61 tcttctagta catctttcat atcgaagtac acatcttcta ataaataaaa atagccttcc
      121 tctatatctt tcaatttatc tatagtatcc atttttttac ttatatcttt cataatttct
      181 aaattttgta tatttacatc tatgattgtt aataagtaca ttctcacagt ttttattatt
      241 tcaacagaaa aatctacaat attataattt ccttcttttt tatataatga ctccttaaat
      301 ttgttaaaac aattatctaa attagaattt atatcgttaa atggtggtct tattatgtca
      361 cgtattatta aaagattttg tatatctaca ttatttgtga ttgcctcatc caaaataaat
      421 aaattatatg catcatttaa tgggtcatgg gcatctccac taaactctaa gttaacaaat
      481 tcaagtaaat ttttcaatga tatatagttc atacatctca taccacaatt tatatatttt
      541 tcttttatat ctacaaataa atctctaatg ttatttacaa accttggatg gttatttttt
      601 ttagcacttc tcatatctgt gttattgaaa catgtcaaat ccaaattacc aaaagtatat
      661 attcctttaa tatcagaaaa tatgccaagc cattttttaa actttctcat gacctcttca
      721 taacttggtg cactcttcaa ttcttcagaa gatatatgtg ttaattcttg tatatgagga
      781 tacaactcct cattacttac tggtcttatt aaagatttaa atttatcaat attttttgtt
      841 ttttcatcat acataactgc tccgattgca attatatcag aattaaatac tgctcttttt
      901 gatttagaat ttggcatatt catttcaaaa tctatatata ctcttttcaa tataaacacc
      961 ctttccaatt gtttatagtg attcttttta actattttta ttcaaaattt tctaagaaag
     1021 aatgtttctc cccatctttt tgccaaccaa gtactgaatc aagatttaca ttttctgccg
     1081 ccttaaatat agatttttct acatttttaa aatttgattc taagctcttt attaagtctt
     1141 ttatctcata tcttttatta cctgtttttt tagcagcaac catacatgca caatttaatg
     1201 gccatatacc actattttga ataaatctta taatatgttc ttctcttata taataaagtg
     1261 gtcttataat ttttatacct tcaaaattag tagaattaag tttaggaagc attgttttaa
     1321 aatttccagc acacaataaa ttaagcattg ttgtctctat tacatcgtca taatgatgac
     1381 ctaatgcaag tttgttacac cctaattctt ctgcctttga atataatgct cctctcctca
     1441 ttcttgcaca catatagcat ggataatctt ttgctatttc atctgctatc tcaaatatcc
     1501 ttgaatcaaa taaatgtatt ggtatattta gatactcaca attatctata agtaattgtc
     1561 ttatatttgc atgatatcca gggtccatag caataaattc aacatcaaaa ttaacttgtc
     1621 catgtttttt taactcttga aacattttag ccataagaat actgtctttt ccaccagaaa
     1681 tcgctacagc tatcttatca ccttcttcaa ccaatttata atctcttatt gccttcataa
     1741 acttagacca taaatttttc ctatattttt ttattatact tttttctata tctcttactg
     1801 gaagtctttc ctcaaaagga actataattt cacagccttt tcctgatagt tcactcatct
     1861 aatcacctcg tatatttatg ttaataatat tttacaaaaa agtctacctt catccacaaa
     1921 atactataat ttttacttta attttactaa ataatttata gtttacaata acacataaac
     1981 ttataataac ataaataacc tctttctttt attatctttt ttatttttct catttaatat
     2041 tttattatta gtaacgattt ttaaatgatt gaatattata cactagatag agttacctat
     2101 ctatttacaa caaggaggtt ttcatcttgg aaaataagac tagagatatc aaaaaaaatt
     2161 gtggtccatc tttggctaga ccatacatta ataatcaaat tttcacagaa atgtactgcc
     2221 tttctgatgc attaaaattt ggaactatat ttccagaact aaacttacta gagtctgaaa
     2281 tgtacaacaa agaattatat gctaagccaa aaaaatatag aggaggtaga agataatgaa
     2341 agactcatct agagctgaac ttatgagaaa agttcaggaa acatcttttg catgtgttga
     2401 tatgaactta tatttagaca atcaccctga tgataaaaat gctatcaaca cttataattc
     2461 attatgtaat caatttgcgc aagctagata tgcttacgaa aataaatacg gtcctttaac
     2521 taactttggg tatgctccaa gtagatgccc ttggcaatgg gctgaccaac cttggccttg
     2581 ggacagagaa tttaattatt agacatcaag tattggagga ttgaaaattc atgtggatat
     2641 atcagaaaac tatacaacac ccagttaata taaaaacttg tgaccctaga atggctaaat
     2701 ttcttataac tcaatttggt gggccaaatg gagaacttgc tgcatcttta agatatttaa
     2761 gccaaagata tacaatgcct actggtaata tgcgtgcact tttaacagat attggtacag
     2821 aagaactagc tcacgttgag cttatatgta ctatggttta tcagttaact tctgatgcaa
     2881 gcccagaaga gttaaaagct gcaggtcttg gttcaaacta tgctcaaaat ggatatggaa
     2941 tttatccaac agattcaaat ggtgttccat ttgatgtaag acctatagca gttatgtcaa
     3001 atcccgtaac cgatttacat gaggatatgg cagctgaaca aaaagcactt gcaacttatt
     3061 atcaacttat aaacctaaca gatgacgttg atgttataga tgtattaaaa ttcttgggtc
     3121 aaagagaaat aattcattat caaagatttg gtgaagcctt aatggatgct tacgagttag
     3181 aagaatctca aaaaatgttc taaaattttt atttgtataa ctattattat ttgataattt
     3241 ataatttact gttgtgttaa aatttataaa aaatataaat ataatttatt ttctataaac
     3301 agagaaaata aataaaaagc aggtatcaat caaatagtaa catgtattat actatacatt
     3361 actatcaata ttgtacctgc tttcatatta taagcaataa agaaaaatct atttattagg
     3421 ttttataata aaaacaatta taaaactctt cttgcattta catatctact tgaatagtag
     3481 ctactattta tattagatat tgttactttt tttgatgaac ctgaagatgc atgtatcata
     3541 tctccatttc ctatgtatat tccaacatga cttacagaac cattatttga accttgtgta
     3601 tcaaagaata ctaaatctcc acttctaagg tctcctctat ttacatatgt tccatactta
     3661 ctttggtctc tagatactct tggtatacta actcctacag attttttcat aacgtactgc
     3721 gtgaatccag aacagtcaaa actatttgga ccttctgctc cccatacata tggttttcca
     3781 agtagtgtct ttgcaaaact tattacacta tcagctcctt gacttggaga attagtcgaa
     3841 taagatggaa ttgaagtatc aacagaacct ttttcagcaa ggtattgact tgacacatat
     3901 ccagtttgat tgccataact tatttttgtc catcctccac tttcagaaat acttccaacc
     3961 tcagtaccat aacttaatgt agctatttta gaatgacttg tacctggtcc tgttctaacg
     4021 ttcaatgact ttgctgttac aacctttgta ccctttacaa ggtcatttga actgttttca
     4081 gaattttcat ttgtagagtc atttacacta gttcctaaat atttacttga aacataacct
     4141 actctaccat tagaacttat ttttgaccat ccatttgact cagatatgta tccgacttca
     4201 gtcccatatc ctagtgttga tattttagaa ctacttgtac ttggtcctgt tctaaaattt
     4261 aatccttttg ctgttacaac ttttgtcgac ttaactgaag tatcactact ttcattgcta
     4321 cttccaaggc tatttattgc tacataattt ccatgcacat aacctgtgtt tccattatat
     4381 ttaactttta cccaagaacc ttcttttcct acgtactcaa ctttatcacc tttgtaaagt
     4441 ttccccattg attcatggtt tgtacctggt ccttttctaa aatttaccgc attccctgtt
     4501 attgttctgc tttctatact cacactctct acttcagact cattagcata tgctaaattc
     4561 cccatagtag gcattattat cgcagttgcc attatactgg ctgctattct cttttgatta
     4621 aaattcataa ctttccactc atctcctctt ttctactatc tttatttcac ttcaaaatat
     4681 aaaagaaata attgacacta tcaaaatatc aattatttct tttatattaa tattattaca
     4741 aatttgtaac tatttgtcta tagtaatatt tgcttaccat aaattaccat gtataaattt
     4801 taatctttaa tagcctgtaa tttagtaata tagccatctt ttaccttttt taaaaatggc
     4861 tatatttttt aacttttttg taacttttta aaatccagtt gagccaaacc caccttttcc
     4921 tctttcggag tcacttaatt cttttacttc ttctatgtta atatcataga taggttttac
     4981 tatcatttga gcaattttca tatgcttttc aaccttaaaa tcatttttac catgatttat
     5041 taatataatt ttaagctctc ctctgtatcc ttcgtcaata gttcctggtg tatttaatac
     5101 agtaacagaa tgctttaagg caagcccact tctaggtctt acctgcgctt ctgtcatagt
     5161 aggaagctct atacatatcc cagttttgat aagtttagtc tctccaggag gtattacaac
     5221 ctcttctata gaatacaaat ccataccagc atcacctttg tgagcaaaat taggtattat
     5281 agcatcatca tttaattttt ttacttttaa attgtacatc ttatatctcc tttcaacttt
     5341 ctataaatat tataacttaa atttcgacct cttgaagttt tttatttttt tgtggtaata
     5401 taattattag tgttttaaag gaggattttt tatgaaactt gtacttgctg agaaaccatc
     5461 tgtggcaaaa actatagcat catttttagg tgcaaagacc agacaagatg gttactttga
     5521 aggaaatgat tatataatta cttatgcagt tggacactta gtatcattat atgatatgaa
     5581 ggattatgat aaagataaat actctggctc ttggaaaatg aacaatttcc catttgttcc
     5641 tgaggataag tttaaattta agattgatag ttctaaaact aaacaattca acactgtaaa
     5701 aaagcttttg aatagaaaag atgtagaata tgtaataaat gccactgata atgaccgaga
     5761 aggagaactt atagcatttt taatattttt actggcaaaa aataaaaaac cagtaaaaag
     5821 aatcttggta aatgaatgga caccagaaga tattacaaga ggtataaaaa acctaaaaga
     5881 tgaagatgaa atgagaaatc ttcaagctgc tggatatact aggcttatta ctgactggtt
     5941 gattggaata aatttcactt ctgttgctac attgaaatac ggaaatggga aactattaaa
     6001 cataggtagg gtaattcttc caacagttaa attagtctat gatagagata tggaaatact
     6061 aaactttgtt cctaaaactt attatgaaat agaagggcat tttaaagctg aagctggtga
     6121 atataaaggt aaatatgtta agggtaaaga aagtaaattt gatacacttg aagatgcaaa
     6181 taaaattata gcttcaatta catctgaaac tggtaaaata ctagataaaa aagttactat
     6241 gtcaaaggaa tatgcaccaa aactttttag tttgacatca cttcaaggat atataacttc
     6301 taagtattcc aattttacat ctgataaggt tttaaatgtc tgtcaatctc tctatgaggg
     6361 taatggtaag ggtggataca taacttaccc tagaactgat tccattttct tagaagaaag
     6421 tttggcttca aaagcttctc aaactttaga taaattaaag gttggtcttg aatatgaaaa
     6481 taaaattaaa ttttctaaaa ctaaaagagt ttttgattct tctaaagtag actcccatag
     6541 tgctataatt ccaacttata taattccaaa aagtttatct aaggatgaac aattagttta
     6601 tgatgccata aaagatagat ttgtagcaaa ctttatgcct cctgctgaat atgagaatac
     6661 agaaattaag actgaagtag ataattgtac tttcttaaca aaaggtaagg tgcttaaatc
     6721 aaaaggatac cttgaggtat ataataagga agaaaaaaac gatttacttc cacttgtaaa
     6781 taaagatgat gttgtagatg tgttagaaat aaaaccatta actaagcaaa ctactccacc
     6841 taaaccgtat actgaggaca cacttttaaa ggctatgaaa aattgtggta aaaatgtgcc
     6901 tgaagaagat acaacagtgc tatctggata ttcgattgga acatcagcca cacgtgctga
     6961 tgtgttaaag aagataagtc aagtgggcta tgtaaagaaa aaaggtaaat cttattctat
     7021 aacagacctt gggaaaaatt tagttgaaat ttttcctgtt aaagacttat ttgatgtaga
     7081 ttatactgga aaacttgaaa aaagtttaag cgatattcaa aaaggaaagt atacaagaaa
     7141 agaatatctt actaatatca ttagttttat atatcaaaat gtcaatatta taaaacaaga
     7201 tgcttcaaaa aatataaaca cagaagcata tactttcgat tcaaaaacta aaaaatttac
     7261 taaaacaaaa gaaaaaaaac agaaaaaaga tgtaactaat actgaaaaag aagttgctat
     7321 atcaaaggat tctaataaca aaaaagaagt taaaaatggt gatgaaaatc aatctcttgg
     7381 caaatgcccc atttgtcaag gtgatgtact agaatttgac aaaggctttg cttgtaaaaa
     7441 tcataaagaa tgtaaatttg ttatttggaa aaatgataag tatttaagtc tttataaaaa
     7501 gaaagttaac aaaacaatgg tcaaaaatat tttaaaaaaa ggtgaaacta aagtaaaatc
     7561 tttaactgct aaaaacggta ataaatttga tgctacatta aaatatgata aaaatccaga
     7621 tactggctat ttcaattgga aaatagagtt tgacaactaa atattcataa gaaaagaaat
     7681 agcagtagta taataatcta tattatatta ctgctatttc ttttcttatg aagtttcaac
     7741 ttttttaata gtgactttta caatcacact ctaacctaat ttctttacta tttctgataa
     7801 ctttaatgta tgttcatcta ataaagttag ttcctcttca gaaagattag ataatttttt
     7861 tactagagat tctttgagaa tagcttttac tgcatcaaat aatttaaatg ctttatctgt
     7921 taattcaacc ctaagaatcc ttctatcttt tgggtcagga aatttattga ctaaaccatg
     7981 ttccactaat ctgtcaataa tcggagtcat atttgatttt aaaattccta aattagaagc
     8041 tatttgagaa attgatgatg aatttgatgt cataagataa agaattacct tagcatgtga
     8101 tggagccatt ggacattctt caaaataagc tttcattata tcagagttat tttgaaactt
     8161 ttttaacaaa tcgctttctt taaatatatt attttcaagc acaaataaaa aatctaaaaa
     8221 agagtccgct attttaagta tattgttgtt atccatattt gtaacatccc ttcaatagat
     8281 aatataataa aacattgttg tttaatatag tataaattat aaactattaa taaatttaaa
     8341 acaagaaaaa gttattgact cataataatt ataaatttat aatcaaatta gagaattata
     8401 aattgccaat aaagggaagt tttttaatta gtctaaaact aaacttaaat tataagacac
     8461 tttaaattct acaacaagct agtgtgcaat atataatagg aggagatatg aaaagatatt
     8521 taatagtttt cttaatgtgt agttcactgt ttttagttgg atgtggaaaa acagaagaat
     8581 ctgaacctga gaaaccaata gcagtttctg ttcaaaaagc aataggtgga gaaattgaaa
     8641 acacaaattc tttctcagga actacaaagg ttaaagatga aacagctgtt actgcacaga
     8701 ctgttggcac agttcaagaa gtgtatgtaa aacttgggca aaatgtaaga aaaggagatg
     8761 aattattgag tataagcagt ccagagctag aaaatagtgt aaagcaaagt aaagcctcac
     8821 tagatttagc aaaagcaagt tattcaagtg ccactggtgg ttcattagag gcacaagtaa
     8881 atcaggcaaa aacagctctt gacaatgcaa agattcaata tgaggagtct caaagaaatt
     8941 atgacaataa taagattctt tatgaacagg aagtcattag tttagaccaa tttaaaaaaa
     9001 tagaattttc tttagagcaa acaaaacaac agttagattc tgctcaaaga gcatacgata
     9061 cagcaacttc aaaaagtata cctcaagcta aagcacttgc taaaaaacag cttgaccaag
     9121 ctcaagtatc atataattta gcaatgagta atttagacaa attaacatta acatctccag
     9181 tagatggtac aataacagct aaaaactttg attcaaagga aatgataaca caatcacaac
     9241 cagcttttat tatttctaat cctaatatac tagaagttga tttaaatgta gcagaatcag
     9301 atataggtaa atttaagaaa gatggaaatg tagatgttat aattgaagac caaaggattt
     9361 taggaaaaat agattatgta ccttcagtag ttgaccctca gacatcttta tatcctgtta
     9421 aagtacttgt aaataataca aacaacaaat ttaaggcagg tatgtctgct caagtaaact
     9481 taagtgttga aaaagaaaat ggtgctgtta cagtacctaa aaaagcaata tttgaagaaa
     9541 atggtaaaaa atatgtatac atagcaacaa aagacaatat agcaaagaaa cacttggttc
     9601 aaactggaat tgtaacagag gataaaatag aaattaaaag tggtgtaagt gataaagata
     9661 cagtgattat cggagggata agtcttattt ctgacggaac taagatattt cctgtagaaa
     9721 aggagaagta gactatgaat ttaacacaga cttccgttaa acgtcctctc acaatcataa
     9781 tggtcttttt agtattaata gtttttggtg gtattggata taaaaaaatg tcaataaatc
     9841 taatgcctga tatagaaata ccagttgtaa tggtcatgac tacttggaca ggtgctggtc
     9901 ctcaagacgt tgatgaacaa gttagtcaaa aagttgatga aagtttatct gccgtttcaa
     9961 atgtaaaatc tactatatca agttcacaag aaagtgtttc tatggtagtt gctcagtttg
    10021 aatttggaac taatttggat gaaataatga atgatgttag gtctaaagtt gatgcattac
    10081 agaccagcct cccagatgat gcagctaaac caacagtttt aaagctggat atgaatgcac
    10141 aggctatagg tcaacttgtc atatctggag gaaatgaaaa tagttcacaa gccttaagaa
    10201 aatacgctga agatgtaatt cagcctaaaa tagaatccat agatggtgtt acttcagcag
    10261 accttaaagg tggagaaaaa gcacaggtta atgtaatagc tgacccagct gttctttcaa
    10321 attatggagt tagtttatcc actataaaag gtgtcttatc ctctagtaat aaaacattcc
    10381 cttatggttc aattactcaa ggtgaggata aaatagtttt aagagcgatt gataaattag
    10441 aatccctaga tgatataaaa cagattcaaa ttcctgtaaa aggaggaaat acagttagat
    10501 tagataatct ttgtagtgta gattatggat atgcagacaa agatagtatt tatagataca
    10561 atggaaaaga cagtttggta atagatattt ctaaacaaca agatgccaac actgtaaaag
    10621 ttatgcagag tgcatacaaa tacgtagatg aattgaataa ggaaaatcct caatttaaaa
    10681 ttaaaattgc taatgataca agttcataca taagtgaatc aataaacagt gtaatgagca
    10741 acttgcttat aagtgctgtt atcgcattta ttgtaatatt tgctttccta aaaagtgtaa
    10801 gagcttcact agttgttgca gttgcaatac caatttctat aataggtgct atagcaattc
    10861 tctactttac tggtgaaagt ttaaatctta ttactgcaag ttcattggta atttcagttg
    10921 gtatggttgt tgataatgct acagttgtaa ttgaaaatat atttaaatat aggaaaagtg
    10981 gcagattgag tcttgatgat tgtgctattg agggaacaag aactgtaaca aatgcaatac
    11041 ttgcatcaac attgaccaca atagcaatat tcttacctat catgtttaca gaaggtatag
    11101 ctaaaataac ttttggggca ttaggtaaaa cactaatagc ttcactagca ttctccttta
    11161 taggtgcaat tacactcgtt cctagtgtat ttgcaaaatt aagcagagga aaaaatagtc
    11221 aaaagatgca agaaaaagat agtcctatat ttgataaggt aagtgaagca tataaaaaat
    11281 tattaagtgt atgtttgaga catcaacgct tagttgtaat atcaagttta cttatttttg
    11341 tagcttcctt atttggaatg ggatttatag gaatggactt tatgcctgca gcagacaagg
    11401 gtgaattatc tatttctata gaacttccaa aaggtcttag cctagaatcc aatgattact
    11461 atgtatctat gactgagaaa aaagtctcag atattccaga aattaaaact ttaataacaa
    11521 ctttgacatc agattcaaat aatagtaaag caagtatagc aattgaatta gaatctcaaa
    11581 aaaatagaaa aagaactact gatgaaatag aaaaagagat aattgataga gtggctactg
    11641 taccagattg taaaataaat gtaagtcaaa acagtagtgt tatgggtgga gctggaggag
    11701 cagacttcac attagaaatg aaaggtcctg atttagatgt attaaaaata ctgggaaaag
    11761 aagctgaaaa agaatttagc tctataaaag gatttagaaa tgttgaaact tctcttacag
    11821 atacaacaca agaagctcaa tttactatag ataaacataa ggctcaagaa tatggtgtaa
    11881 atactgctga aattgcaggc atgttacgta cagcaataaa tggtgactct gtaaccactg
    11941 ctactattga tgattacaaa gtggatgtaa acctaaaatt taaagcagat actattgata
    12001 gcttagaaga tataaagcaa ctaaaagtaa actctgcaac tggaaaagaa gttcctcttg
    12061 gtgctttttc agatataaaa atggcagatg gtctacaatc tatatctagg actgatggag
    12121 atttttctgt aaatataact gctacacttg acaatttaga tgctggtact gctacaaaac
    12181 tagcactaga aaaaacgaat aaaatggtaa ttcctaaaga ttactctata ggtgttggtg
    12241 gtgaaacaga aatgatgaat gaatctatgg caagtcttgg attttcaatg gtcatagcta
    12301 tagcattagt ttatatggta atggtagctc agtttgaatc atttagtaaa ccatttataa
    12361 tcatgttctg tgttccattt gcattcgttg gagttgtgct ttcgcttcta gttagtggtc
    12421 aaagtttaag tactgtcggt atgctaggta taatcttact tatcggtatt gttgttaata
    12481 atggtatagt tcttattgac tacatagagc aacttagaaa aacaaatcgt aacaatgatt
    12541 taattggttt ggtttcaatt ggttgtgcag caagattaag accagtctta atgactacag
    12601 caacaacaat tttaggtatg ttgccaacag cactatctct tggtgaaggt ggagaaacta
    12661 tgcaaccact agccatagtt ataatgggtg gtctaactgt atctacactt ataactttgg
    12721 tgcttatacc tactatatac ttaatatttg ataaatggga aaataagttt tcagacaaaa
    12781 tgcctaaaat atttggtaag ataacagata aaataggaag tataaaacgt aaaataataa
    12841 agaaaccaaa attgaaagct aaagtttaaa tagaatggag ggtgattgta ttgaataaaa
    12901 aaatgagcaa aatagttgca attggctgtg gtgttggtat aataactgcc aatttaatcc
    12961 cagtttacgc agataataat acttcttcta taaacaaagt tgataattct attttaagtg
    13021 actcaaataa aagaacatct gaaaaggaaa ttttgactgc tagagaagct gttagtgctg
    13081 cacttaaaaa tagtgaaaaa ttaaaaatga aatcagaaga aataaaaatg ttaaaagaaa
    13141 agttagaagt acaagatgag tttgatagtt ttactggaag tgacaactcg tttccatacg
    13201 accaaattgg acttcttaaa aatcaaagtg aacaggctaa aggctttatg gaggaccaaa
    13261 tagcaaatga tattactaat aaatttaacg atttagtttc aagagaaaat gagctagata
    13321 agataaaaaa caatcttgaa ataaaaacta aagaaattaa agatatgaaa ttaaaaaaag
    13381 atttagggtt agtaacatct ttagaaactg aatcagctga acttgaactt caaacactac
    13441 aaaatactca aaaagctaaa cttcaagaac taaaaaacaa tcaagactat tttaagttat
    13501 tgactaatat agatttaaat aattatcaat tggataaaga atatagattt gaatctttta
    13561 gagtaggtgg ctcagttgat tcatatatgg aaggtaaggt taatgaatat ttaaaatacg
    13621 accaattgat tttagaacgt acagaagaaa gctttaatga taaagatgag aacaaagcag
    13681 atttaccaga tagacctaac cttacaagac cagttgctcc tacaaaacca gagcaaggta
    13741 acctaactga tgatgaatac aaggttctta tggataagta tgaaaaagat tatgatagat
    13801 ataaaaaaga agttgaagca tacaatcttg aaagacagac ttatgctgct ggtcttacta
    13861 cttatgcaaa ttatcttcaa caaaaattta atactgaaaa tggtctagta acattagaag
    13921 acagtaaaaa agctcttaaa aaagggttaa ttgatagcta tgctcaatta cttgctttag
    13981 aagatacaat tcagattact aaaaaacaac ttgatttatc agaaaaacaa ttaaaaaata
    14041 ctaaattaag atatgattta ggattaatta ctctaactga ttataaaaaa caagtggtaa
    14101 gtaatgaaga tgctaaaaat agctatgact ctcttattgt gaattataac tcactaaaaa
    14161 atggtataga aaaaccttgg atacttaata ctggaaaata attctggtaa atagaaagat
    14221 tactttaaca tacgtttgat taaaaaatag ttatgcacac tcgtggcttt agtcatggaa
    14281 tattcagaag gcaaataata gaaatagatt agtaaaaata tatttactta taaaactgta
    14341 tacttttata gaggctatct caaaatagag ataagcctct tttttattgt ttaaattata
    14401 aaaatatatt tgattccata caaaaagagt gccttatgaa ctaaaaagtt cattttgaga
    14461 caccctcaaa agcttaactc taaatttaat tattttttat caaatgtctc atctatttta
    14521 catctcacca tgttaacaaa ttcagaaata ttatatcctc taagtttgca tccacattta
    14581 ggacaataat ccagtttcct agataccaaa gccctacaac taggttctgg acatcttatt
    14641 tttagactct catctgctac tttaaatcca catcgtgggc atatactacc tggacgtgat
    14701 gatacttcct ttccacattt aggacacttt gtcaacttta tcccctccag taaactcaat
    14761 ttaattgata gttgaaattt ctctctacaa taatatatgc aatataatta aatacgtttc
    14821 acttacaact atataagtta aatttttata ataaaaatcc cctatattca atttacaaat
    14881 acttaatttg atacattgaa caatagggga ttttattttt atttagttac atattttgca
    14941 gtctattatt atctaggatt ttttatagat gcttgagctg ctgcaagtct agcaattggc
    15001 actctaaatg gtgaacaaga tacatagtta agtcctacac tataacagaa ctctactgaa
    15061 gatggttctc ctccatgctc tccacatatt cctagtttta attctggttt aacttctcta
    15121 cctaatttag ctcccatctt aactaactta ccaacaccat tttggtctag tacttggaat
    15181 gggtcttttt ctaatatttc ttcatctaca tattgaccta agaatttacc agcatcatct
    15241 cttgaatatc caaatgccat ttgagttaag tcattagtac caaaactgaa gaagtctgct
    15301 tcttgtgcta tttcatcagc agttaaacaa gctctaggta tttctatcat agtacctaca
    15361 gtataaggaa cttctatacc ttcttctttt attatagcgt caacagtttc tactatcatt
    15421 tttcttatta tttttaactc atttacttct ccaactaatg gaaccattat ctctggagaa
    15481 actttaacac ctgctttgat tgcttctata gcaccttgta ttatagcttt agattgcatt
    15541 acacatattt caggataagt tattgcaagt ctacaacctc tgtgtccaag cattgggtta
    15601 aactcatcta aatcaactat tctcttctta atatcactta cttttattcc catagatttt
    15661 gaaagttctt ctatagtctc atcatcatga ggcaaaaatt catgtagtgg tgggtctagt
    15721 aatcttatat taactggttt tccatccata actttaaata actctacaaa atcctctctt
    15781 tgcattggta atattttttc aagagctttt tctctttgtt ctactgtatt agataatatc
    15841 atttctctaa ctgctggcaa tctagcttca tcaaagaaca tatgctcagt tctacataga
    15901 cctatacctt ctgcaccaaa ctctatagct gcacgagcat ctctaggatt atctgcatta
    15961 gttctaacca tcatacattt gtgtttgtct acccagttca ttaatttttc aaaatttcca
    16021 gttaaagata cttctgtttt cttaacatct cctaggtaca catttcctgt agaaccatct
    16081 atagaaatgt attctccttc ttttataact acatcatcaa gtgctcttat ttctttatta
    16141 aactcatcaa cttttatttc tccacatcca gcaacacaac atttacccat acctctagca
    16201 acaactgctg catgtgatgt cattccacct ctagctgtaa ggattccttc tgctttaacc
    16261 attccttcta tatcttctgg agatgtctct agtctaacta aaactacttt ttctcctttt
    16321 tcatttgctt ttactacatc atcagcattg aaataaactt taccacttgc tgcacctggt
    16381 gatgctggta aaccttttgc tataacctta gcttctttta atgccttatc ttcaaatttg
    16441 ggatgtaata attggtctaa ttgattaggc tcaattctca tgatagcttc tttttcatca
    16501 attattccag cttcaactaa atcaaccgct acatttattg ctgctgctgc tgttctctta
    16561 ccatttctag tttgtagtat atataatctt tcattttcta tagtaaactc tatatcttgc
    16621 atatctttat aatgaccttc aagtatatga gtaattttta caaattcatc gaatacagct
    16681 ggcattacat ctgcaagagt tgatatattt tgaggagttc ttatacctgc aacaacgtct
    16741 tcaccttgtg cattcattaa aaattcacca aataatttat tttctcctgt agctggattt
    16801 ctagtaaatg caacacctgt accactagtt tctcccatat taccaaatac cattgattga
    16861 atatttactg ctgttcctaa gttatgagct atatcattca atttacggta tacgattgca
    16921 cgaggattat tccatgatct aaatacagcc tctattgcaa gcattaattg ctcttttggg
    16981 tcttgtggga aatcattttt tatttctttt ttataaattg ctttaaattc ttttactatt
    17041 tcttttaaat catcagttgt aagttctgta tcaactgtgt atccctttgc ttcttttact
    17101 ctgtctaata cgttttcaaa tttatatttt ggaacttcca tagcaacatc agaaaacatt
    17161 tgtatgaatc ttctgtaact atcatatgca aatctttcat tttgtgtagc ctctgctaag
    17221 ccaacaacac tattgtcatt aagacctagg ttaagaattg tatccatcat tcctggcata
    17281 gagaatactg caccagaacg aactgatact aataatggat tcttattgca tcctaatgtt
    17341 ttatttaaat ctttttctaa agtagctaaa tgctcttcta tttcttttat tatctctgct
    17401 cttatacttt cattgttaac ataataatca ttacacgctt ctgttgttat agtgaatcca
    17461 ggagggacag gtaagcctat cttagtcatt tctgctaaat tggcaccttt acctcctagt
    17521 aaagatttca tgtctttgct tccttcacca aaactataaa catacttagt ttccattgtt
    17581 aaatccccca ttaatctttt tattttaatt tatatcattt ttattaatag acattattaa
    17641 atttctattt ctttgtatat ctttagacca ttttctttca ttatgtctaa aattatacct
    17701 gctgtttctt ctattgcctt atttgaaacg tttattactg ggcatccaat tcttttcatt
    17761 atctcttctg aataatctaa ctcctgaagt attctttcta aattagcgta gtttgcttta
    17821 ctggacaatc ctaaagcttt taatctttgg gttcttatct cattcagctt ttcaggtgaa
    17881 ttagtaagac ctattatctt tttagtatct atctcaaaca cttctttagg aattggtatt
    17941 tctggaacta atggaacatt agctactttt atatttttat tagctaaata catactcaaa
    18001 ggagttttag atgttcttga tattcccact aaaattatgt ctgcttgaag aacacctcta
    18061 gggtctttcc catcatcata tttcactgca aattctatag cttcaactct cttaaagtaa
    18121 gattcatcta attttctaat tataccagct tctcttttag gttttcttcc tgttctcttg
    18181 cttatttctc ttagtatatc tgtcattaaa tctatacaac taagaccttc tctttcgcaa
    18241 tatctttcaa caatactgca taattcttca tcaactaatg tatatacaat taaagcgttt
    18301 tcagcttttg cattctccaa agtttcaagt agtactcctt tattaatcat gtatggaaat
    18361 ctttttattt cataagtttc attcacataa aactgagaca tacatgcctt tgcaacttgt
    18421 tgtgcagtct cccctactga atctgatact gcatatataa ttaggttttt caccagcata
    18481 cctccttaat tgtcaactat ttctaagaat agcttagtta tgttggtttt tgatattctc
    18541 ccaacaactc tcatatgatt tttgtcttct cccttatatt caacaatcgg aatcgaatct
    18601 acttcatgtt ctattatttt tcttgaagct aataaaacac ttgctccttt agttaaagtg
    18661 actacatttg gagttcttgt cattatcata cctattggca ttttatttat atcagaacct
    18721 cctatagttg cttttagcaa gtcttttctg gaaacaactc cacataactc ttcattctca
    18781 tctataataa ctatactccc tacatcagat aaaaacatag tgactattac atcatatata
    18841 ctttcatcct tcttagctag tacaggcatg ctcattatat cctctactgt tttttctttt
    18901 acacttttac caattaaatt tatttcacta actcctgaat aaaaatatcc aacttttggc
    18961 cttgcgtcta atattcctgt cattgttaat atagctaaat cagatctaag tgtagcacga
    19021 gtcacattca agctggaggc aatactttca ctcgttattg gttcattttc cttaactata
    19081 tctataattt ttagttgtct ttcattaagc tgaataataa tcaccttctt tcattgtgct
    19141 atacttttta tcttttcgca tcttatatag tatattatat aactatatat accattctgt
    19201 aaacagattt tttattgaat acgatatatt ttttactaag ttctctcgct taaagatatt
    19261 tattatatat taatggggga tatttcttta attaatagaa ctatattagt attacagtta
    19321 atattataat acataattgg tcattttaat tcattttata ttaaaatttt taataatatt
    19381 gtttcaatgt gaatataaaa ttgcagaact aattcaaaat aatttttacg actacaaata
    19441 acctataata tacttttaaa gaaagtatcc taacaagaat atataaaaat ttaaaagggt
    19501 tgatttaaaa ataaccatca acccttttta ctaatatcta tttatattac catcaaatct
    19561 tacccaaata ttttttcttg caattgtctt aaatgatata aagtagtatc tttttctatc
    19621 tctaccatat cgtaagttat aacttcacct tttttaatat cttttacaac tttagtcttt
    19681 ttacttatca aaccaatagg tacagcattc atagcctttg ctacatcgta tttttctata
    19741 gtaccatagc atgtatatgc tccatagcca tccataaact cacctttttt caaatctttc
    19801 ttagctatag tcataacttc tgctactagt ccagctttag gtactattgt agccttatgg
    19861 tctaaaactg ccatagctgc tgataaagga gtctctaaac tacataaatg atatggtcta
    19921 tataatatgt aattaggtcc atcacccata cttaaatatt ttagttcatc attaactgct
    19981 tttaatttat gggcaactac tacgaaaact ccaggtgcta taccatttac aaaatcaact
    20041 acaccataat tatctaatac tccaccttcg ctttttaatg catatttctt aggtacttca
    20101 ttaactgtag cttcaatacc atgaccacct cttacatcag gcacaaatcc agttgcatta
    20161 cacattaaag ccatttctac cattgtttta gttccttctt taaaagaagc tatcatatga
    20221 ggtgaagctc cttttctttc tgcttcttct ctaactgtat caggattaca atctaaatct
    20281 aatttattat ttttaccttt acctatcact ctaacttcaa accccattgc atctgcaaaa
    20341 tcaaataatt ccattactgc acctggctca tctcctgcag aacctgtaaa tattactcca
    20401 gcatcatctg ctaatttttt aagtattggt ccaacaacac aatcagtttc agcatttaac
    20461 ataactatat gttttttatt taaaatagag tttaatgcta tctctgcacc aattgcaata
    20521 cccccagttg catctataac tacatctatt gacttagttt tacaagccac aagaaagtca
    20581 ttacatgtca atacttttcc ttcttgtaat tttatttctg catccttttc atttgttaca
    20641 ttatctataa tttcttcatc tattcctgca tcagtaaaag ctttatgagc caactccaaa
    20701 tttatatcta caacaataga agcttcaata cctttcatat ttgccatttg gcttaccatt
    20761 ccatttccca tatgcccagc tcctataaga gctactttta tttttttacc ttcctcttcc
    20821 aattttgcaa gttttctgtt taaatctaac ataccaattc ctcctgttat aataatttta
    20881 ttttcaaatt aagaaatagt tattaaatct ccaatagtta tatctggatt tcctatttcc
    20941 acatgtattt ctcctggtaa atttacttca cattttccat caaatttgat tgtacaatgc
    21001 ccaatttctc taagagtctt ctgagcaata tcaccaactg aaactataca ataatctcta
    21061 ttccctatag taagtgtatc tcctatttca acatcacttt ttaatgttgc tattgaatga
    21121 attacagata tatcctttaa gtcgtcatca ttaatggttt catcatatat aataagacaa
    21181 ttgctttcat ccattaactc tttaactaaa ctgcctatcc cacttatctt aacttcatat
    21241 ttcatgacat tcctcctgat aatttatatt tatattgagt taattaaaag aaacatttat
    21301 tttttctttt ataaatttta tagcttgtac aatactctct tcatctttaa atttttgctc
    21361 tgcactatat atatctaatc cttctatttc ttcaatctct ttaaatttag ccattactgt
    21421 acgccctttt aaaatttctc caaactttat aaacccatct gaatctgaag atataatggc
    21481 tatacttcct ttagaaaaat agcttttctt tttgcctgta gacactatat cacccttttt
    21541 tttagctcta tttaaagttt taaaatacaa tttattctgc ttcattataa atatagtatt
    21601 taaaatataa gcaacaaata aaattactaa taatttcata tttatacacc ttccaaaaat
    21661 cacattattt ttaaatctgt ataacaacta taaatgctgt acaatagtta tataaggttt
    21721 ttaagtaaca cctttttaaa ttctaaaaaa gttctacatt tacttatctt attcgttaat
    21781 tttacatctt ggcctatctt taaaacttct tcataaattt ttactaataa gtctgagtct
    21841 atcttattct cacatggaaa cattgtcata agaattattt ttatacttct atttgcatat
    21901 tctattggtt cttctaaaat tccaactgcc attaaaaact tatctgattt ttcatttata
    21961 gaatgaggaa ataataaacc tttatcaaat actgtgggag atttttcttc tctattgact
    22021 atattttttc taaacttctc atcaatacat cccaaatcca taagatggtc catcattttt
    22081 tctaatgagg caagtatagt cttttcattt aatatcataa atttatcttc atctaacaaa
    22141 ttatttatta gtaaatttgt agaattagta gtgtcaatat tcccttctct taggcacaat
    22201 actgttttta attgttctct tagtttgttt tcatcaaata ttgtatttat ttttaatact
    22261 attgtattta tatttgatgt gtctaaatca atagtcgtaa aaactatatc gtattcatct
    22321 aagttaatat ttctcaaatt aaaacttgaa agagtatcaa ttttaggatt atttcctatc
    22381 agtttttcta ctcttatttt caatagtttt gctgttccta gacctgtact acatataatt
    22441 gctatttttt ttattgaata gaatctactg ctatttcttt ctaaatatcc actgaaatgg
    22501 attgctatat atccaatctc atcttctgaa acttctaagt tatacttttt ctctattact
    22561 tttcctgcta tttgtgctaa tttaaatgga agtatacaat tttgcttaat ctcttctaac
    22621 aaaacatttt ttaactttat attaaataat agcctgttta aagtaaaact aagatgatat
    22681 cctagaccta ctcttaattc atcctcattt ataaaaatac ccatatcgac atatatttga
    22741 ttaaatatat tttccatcaa ctcatcaatg tttttactaa ttctactact atttaaagca
    22801 cattctgatg ctggtgcatt tccactaact aacggtatag ttaaataaat aatttcttca
    22861 tcatttaaag tacattcaaa cattcctgat attatatgtt ttatttccct agcaataaag
    22921 tattcatcat atgatttaag taaatcaaac tttacatcat aatcttttac tttcttttct
    22981 tcttttactc tatagactaa aactactatg tagttaataa cttctcttaa catttcatct
    23041 gtaacataaa aattattttt tttaaataaa gtcaatagtt tttcttctaa atttatgatt
    23101 tcttcttttt tgattccttt aaaatacttt gaattactta aatcttttct tgagttttta
    23161 tacaactgat ttaatataaa cagtctaata tcaagttcat ttccatttaa tttcatacct
    23221 gtattctgtt ttttaatcaa gtttaaatta tatgaagcca aaactttttc tacatactgg
    23281 aaatcattta ctaaagtagt tcttcctaca cacatctcat cagctaaact atcaagtgta
    23341 ataaatccat ctagttctat caagtaattt agtataaact ctactctatc ttcttttgaa
    23401 tttaatgaaa atacattttt tctattcata tcaataatct tattgagtat ctgctcatct
    23461 tttatttcta gtttatagcc ttgacccttt atatatttga ttactgctat atcattacct
    23521 aaatctttat tcaattgttt tatatacgtc cttacacttc tagttgtgac attcaattca
    23581 tttgctattg agttaatact cacaaactct tttttactac acataatcaa tatcttaaat
    23641 atcttgcggt caatagaagg aattttctca gttacactca ttttgtatct cctccttgat
    23701 ttcattctag tacaaatttg aaaccaaatt aatacataaa atttcacttt aataaaggaa
    23761 aatttagtat taatctaact cgataattac agctaattaa tagtataaaa gctagtcttt
    23821 cattaacaaa aaatcttgac tagcttaaat ttatatttac aattttatta attaattcca
    23881 agccttatat tgacattaaa tattatctca atgcaaatac tgcaactcca tatgcaataa
    23941 taacagctat tggaccagtt attaaacgag agtaaaataa agctggaaca ccatactcta
    24001 cagtttctgg ttctgcttct cccaaactta atcctactgg tacaaaatca catccagctt
    24061 gcccatttat tgcaaataaa gctggaagtg ctaaatatgc tggtattgca ccaagaccaa
    24121 tttgtgtacc tacaagagtc cctactactt gagctataac tgcaccaggt cctagtattg
    24181 gtgataagaa tggaagtgtg caaattactg atataatcaa taatccacca atattacctg
    24241 caagtggaga tataactctt gcaatccagt ctcccagtcc agatgctaaa ataattccca
    24301 taagcatact tacaaatgcc ataaatggta ttacgttatt cattaccatt tggatagtat
    24361 ctcgacctgc atcataaaat tttgatacta cttgccctgc cccttgacca atagaaagta
    24421 ttatgttctt tttagcatat tcaccttgtg cttttgactt aacctcatca taattatcag
    24481 gctttctaat agaactctta ttttcgcttt tattttcttg agatttttgt ggcatatctt
    24541 ccccatctac aacacttata cagttaggat taacatctga tacataatat tcttctgtta
    24601 taaattttgc taaaggacct gtttttccaa ctggatttac atttattgta ggtatttttt
    24661 tctttggata aactccacat ctagcagttc caccacaatc aataataact actgctactt
    24721 cttcatcaga aactccagtt ttaaatccat ctacaatttc acaacccagt agttctttta
    24781 tttttaatgt tacttcatgg ataccgccag cagtcattga tagtatctta tttctattcc
    24841 cttctttttt tatatacaaa ggacctcccc agcctgaagt tcccttttct atttttaata
    24901 ttttattctc ctccatttta tctacctcct aagccttatt tgttgctcta ttttttcttg
    24961 ttagaaaagc atacattttt tctgttacta tacctcttat aaaaattaca attagaccaa
    25021 ctataaaata ccacaatgcc agtgtagtag tttctttacc taaagtagtc aaacctgctg
    25081 atattcccaa ccatacaaaa agttctcctg catttgcatg tggaaaaaga cctgtaattg
    25141 gatgtacaaa tgaaactgct gcatcataaa atcctgcttt gtgctcttct tttaaaaatc
    25201 taccaaatgt atagcacata ggatttccca agaaaaatac tgatactaat ggaagtagaa
    25261 tatatcttgt aaaagagttc tttcctagct tagaagccca attattaact ctttcctcac
    25321 caattatctt aatcaaagag ttaatagcta ccattaatac aattagcgtt ggaagagtac
    25381 ctgaaacaag acctataaat tgttctcccc ctttctgaaa caaaccgata aaccactctg
    25441 cacctttact tagacctgta ataacgtttt ccataaaatt ataacctccc tataatatct
    25501 ttaaattttt aattttactg cttaccataa attttacaaa ttacatcttt tttctgtact
    25561 atatgctaat attgtaaatg tttttttatt aatatcattt taatataata tttttgataa
    25621 ataaatacat cttttttcaa ttttataaag gaaaaaattg tactaattag a
//
LOCUS       DAGSRM010000048        25041 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.048, whole genome shotgun sequence.
ACCESSION   DAGSRM010000048 DAGSRM010000000
VERSION     DAGSRM010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25041
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.048"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(470..1008)
                     /locus_tag="MJE33_002168"
                     /pseudo
     CDS             complement(470..1008)
                     /locus_tag="MJE33_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427977.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
     gene            complement(1085..2890)
                     /locus_tag="MJE33_002169"
     CDS             complement(1085..2890)
                     /locus_tag="MJE33_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY9648101.1"
                     /translation="MLSKLKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEASKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIALKKLDIDKNIIENIVNIVSVESSSNGHELLEI
                     DIDNKIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHGKVNSGWGNSDIESIIGNS
                     EAMLKVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPE
                     NLLESELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQER
                     KFARIGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDD
                     IEAIMMKFASKYSLELGIQLNKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDG
                     IIYKDMLPLDILNYYNINGNIYKNKDVNIIFEDDIVGGIVENQERILSIKELELTYIN
                     KLLNKYGRDTKTKKKIAKDLGIGLATLYRKLEEEQ"
     gene            complement(3286..3663)
                     /locus_tag="MJE33_002170"
     CDS             complement(3286..3663)
                     /locus_tag="MJE33_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBY9648102.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVKAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
     gene            4568..5053
                     /locus_tag="MJE33_002171"
     CDS             4568..5053
                     /locus_tag="MJE33_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648103.1"
                     /translation="MSKSELTAETTEEMLEVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEECSKTIKDSITKT
                     RTCKKCNSKCIGGSSFELDGKSYLTCIGSGHYFANMEEMDWKNLEKLITKENNIMQES
                     V"
     gene            complement(5253..5792)
                     /locus_tag="MJE33_002172"
     CDS             complement(5253..5792)
                     /locus_tag="MJE33_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBY9648104.1"
                     /translation="MSKNVLIVSASPRKGGNSDLLCDQFIQGVLESKNQVEKIFLKDK
                     DINYCTGCGACYEKEANCSQTDDMGEILEKMIGADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVTAADENVADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNV
                     GDIKGSKAMKQAYEMGKLA"
     gene            complement(5785..6198)
                     /locus_tag="MJE33_002173"
     CDS             complement(5785..6198)
                     /locus_tag="MJE33_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBY9648105.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIKARKELLIEQRNQLAKRIEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKKMEAVSYE"
     gene            complement(6234..7355)
                     /locus_tag="MJE33_002174"
     CDS             complement(6234..7355)
                     /locus_tag="MJE33_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBY9648106.1"
                     /translation="MEAKKLGFGLMRLPIKDENDVTTIDMEHLNKMVDTFLERGFTYF
                     DTAYVYHMGKSEIALRESLVKRHKRESFTIATKLPLMALKKKEEQETIFNEQLEKCGV
                     DYFDYYLLHNIGVSHYEVAKKFDSFKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEH
                     PEVDFVQLQINYLDWDNESIQSRKCYEVAEKHNKPVIVMEPVKGGTLAKIPEKAEKLL
                     NDYNPDMSISSWAIRFAASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFVQEEYNIID
                     EAVEIINESILIPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNY
                     TKTYGKASDCIECKQCEESCPQHIKIIDALKNVAETFEK"
     gene            7954..9606
                     /locus_tag="MJE33_002175"
     CDS             7954..9606
                     /locus_tag="MJE33_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HBY9648107.1"
                     /translation="MNKKNLAILISGMMFFSSSSAIFADNTTKQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIENSTKAELKRLGV
                     DNVYIVDSGNSISSKVENEIKSLNIKINKIVGNNIYEMSTNVLKEIDKIKKVENVAVV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSINFLNNRSIKKSYIVGGNKILSD
                     NTVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGINQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIKNKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKEILKQALPGSNILVCYYDEDDNEYDCKVIKDNILYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTQNISLDKAKSIALDAIPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIVDIDMDNDSDDDFDDNDKNNSNTNDNSSIISAEKAKSIMLDKVPGGK
                     IVKFKYDEDDKEYEGEIIKGKTEYEITINAITGKILEFEIDD"
     gene            complement(9965..10891)
                     /locus_tag="MJE33_002176"
     CDS             complement(9965..10891)
                     /locus_tag="MJE33_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBY9648108.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDVNRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKAASDLDVSISTISRLVNEFIDLGILRLISRGVKGNCCSVYSYVCSESS
                     NAEIVTKNGDIFKNIEKYIDATINNNQKTMKYKHRNELFKNSNTNIYSRINQESIDKD
                     YDLIIHTGYETENETKKKELLKNNLNKKNLNKNTFENSDEDLTEDSTYKNPESIHEII
                     IRKLNSESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEP
                     TILFGYEFESYLNEESSLSACNYGDMCYEDMY"
     gene            11481..12401
                     /locus_tag="MJE33_002177"
     CDS             11481..12401
                     /locus_tag="MJE33_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBY9648109.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLSLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTVTDIAHYFGFTDSAVFSRAFKN
                     YYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSNGNYKLRA
                     SFPFEVYLNNPHNHPQNRHLVDIYLPIEPF"
     gene            12487..14907
                     /locus_tag="MJE33_002178"
     CDS             12487..14907
                     /locus_tag="MJE33_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBY9648110.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLTLLYSVEGQIMCTMCDTPVSDNLICNNCGYEEERLRPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRAKITPGYLRVFSKKFNDYLDIQFLQLPEEIRNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDDFLKLLINQGNLNTFGTNLVNDILSKTQHLIESRLGHLSLYREM
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSLKKLKDLGNTVI
                     VVEHDKNTIEMAEHIIDIGPKAGVEGGQLVYQGDLEGLLQSDKSLTGQYLSGKYPMPN
                     RTSNKNITHTDKIPCITIQNANTNNLKNVTVSLPLGAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQINSSESEIGEEDDYAVVETIADRLIGTKHISGYSEISQSPIGK
                     NMNSTPASYIGIWDKIRTLFAGQPESLEQGFTAGHFSFNSKGACPKCSGSGYEKVWLG
                     NNLSIDHICSECQGKRFNDESLSIKYKNKNIHDILNMSVSESVHFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPTLSGGESQRIKLAKEIGKKRNGNILYVLDEPTTGLSLYDTSK
                     LIQLLDELVENGNSVIVVEHDIDVLNVCDWIVELGPEGGDKGGYIIAEGSPKTLKENP
                     KSITGRYL"
     gene            14907..15929
                     /locus_tag="MJE33_002179"
     CDS             14907..15929
                     /locus_tag="MJE33_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBY9648111.1"
                     /translation="MNKNNCLDLIFEKVDPSSVGMSSEKLSQLEPLIISQYSNINGII
                     IARKGKIVFEKYYNGYNADDTFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPE
                     YTCSPSEICKKAVTIRHLLTMTAPYPFKNMTESLERLCKQKDWIKYTLNQLGQKGEIG
                     TFKYSSAGTHLLSAIITKTTGKSAREFANEYLFKPIGMKVIPDYDMKDFGFEDFFGKN
                     LKGWAKDRQGYSIGGWGLTLSARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSN
                     EYGYLWWLCKKDGVLWYSAMGTGGNAICCIPQYDLVIAIPSKIVFKPRDRWEFIEKYI
                     LTSIID"
     gene            16082..16828
                     /locus_tag="MJE33_002180"
     CDS             16082..16828
                     /locus_tag="MJE33_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBY9648112.1"
                     /translation="MDTYTTSEVADLIGVHPNTVRLYEELNLIPKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAISLVNQYLQQLEIEKSN
                     AEEALTIVAQILNGNTPETNKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSSPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKSHLQIMKKQFS"
     gene            16914..18164
                     /gene="cme"
                     /locus_tag="MJE33_002181"
     CDS             16914..18164
                     /gene="cme"
                     /locus_tag="MJE33_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HBY9648113.1"
                     /translation="MENKNTNSTWKKKVILFLASQYISLFGSSLVQMAMIWYVTLQTS
                     SGVWVTVLTVCSFIPQMIISFFAGVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGVQTPAVNAMIPQLVPEEHLMRFNGINSSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHDIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIGFLGMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVISINNLIIIAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
     gene            complement(18681..19694)
                     /locus_tag="MJE33_002182"
     CDS             complement(18681..19694)
                     /locus_tag="MJE33_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9648114.1"
                     /translation="MIGLRYQLKSIKKDKMCIISFFLPVVMAILINFMGTIDISSLGE
                     IRFGIVKDTLPVQTEQWLKKYGMVSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTVF
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTILPKDSILKEFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEVLPQSRSQYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGTPLLIYLLGVN
                     TSGIGNLCYIMPSVATFNGIMEIINGNVIIIKDIVILAVHCVVWFMIYIILDKKRKLS
                     FSR"
     gene            complement(19697..20725)
                     /locus_tag="MJE33_002183"
     CDS             complement(19697..20725)
                     /locus_tag="MJE33_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9648115.1"
                     /translation="MYSKFFTLVKKDFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSASPLINQVSSIGELKAKLDEDTSAVCIDMSSSKPKVIL
                     HESIKGVDNYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKDMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFFICDLCFTVLLVV
                     FNIGFLEGIKILPQVLVQASILSIIMSLIGIGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYSQLKNAFFMNFSKELVYFVVCSLTIIVLFLLVKYIF
                     NKELVREG"
     gene            complement(20725..21570)
                     /locus_tag="MJE33_002184"
     CDS             complement(20725..21570)
                     /locus_tag="MJE33_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9648116.1"
                     /translation="MIKVRELKFSYGKDKQILHGLNFEVKEGEIFGFLGPNGSGKSTT
                     QKILNGVLKGYNGHVSIFGKEVKAYTESLYQKIGVLFEFPYLYTNLSAIDNLEYFSSF
                     YPKNQRRDIYELLDLLEFKKEFINKPVSSYSKGMKQRISMARALVSNPKLLFLDEPTS
                     GLDPSGAVLFRKIIEEERKKGTTIFLTTHNMLDADLMCNNVAFIADGKIMVIDKPKNL
                     KMKNSNNKVEVEFVYNGNRDIEIVDIQELESGITFKYDEILSVHSKEPTLEEVFIKYT
                     GRMLV"
     gene            complement(22001..23131)
                     /locus_tag="MJE33_002185"
     CDS             complement(22001..23131)
                     /locus_tag="MJE33_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY9648117.1"
                     /translation="MNSLRKIIEKLKNVDISNWIKQNFGLYSVRKKVFLLSKLSGALI
                     ILFYLVVEEFSINSKFEFWIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQQALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDTISHEMKTPLGIIRAYTEGLIDEVDEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVADKMRMEQVV
                     ENLVINAKKHVVYNGNLDLSITCENGLLLFEIYNDGRFIEQDEISKIWSKFYRSVQSQ
                     RTGGSGLGLAIVSQILTMQGLEYGVNNRDRGVEFYFMIPIDE"
     gene            complement(23128..23799)
                     /locus_tag="MJE33_002186"
     CDS             complement(23128..23799)
                     /locus_tag="MJE33_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY9648118.1"
                     /translation="MAKILVVEDEKRMQNIIVEYMQKGGYTCITADDGVEALTILKSN
                     NIDLMILDIMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTDIPKNSLSLGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHHIVTLIRSGYK
                     FEVKE"
     gene            complement(24002..24652)
                     /locus_tag="MJE33_002187"
     CDS             complement(24002..24652)
                     /locus_tag="MJE33_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBY9648119.1"
                     /translation="MDFISSILQGLLSILFNFTGDFGISIVLITLIVKLVLMPLSLKQ
                     RFSVKKQQELAEKMEYIKEKYKNDTKELEKQIQVSSVESMKSMLGCLIVLVQMPVVYA
                     LYHVCLDMTKEFTTIIIPWIANLGTFDNLFILPCIYTLIMLASNLVNYIPYLKINSQV
                     KFNKQMAVVTTITSLILTVRTPVAIGLYFITSAIYSLIEDICFRIYFNRKNRIVTK"
BASE COUNT         8862 a         3967 c         3053 g         9159 t
ORIGIN      
        1 tttaaagttt ataaattttg agtgtgaaaa ataagattta taaaagaaaa aattctcata
       61 ttgggaaatt tttatcaata cgagaattat caattcgaga aatcgtttat ttgtcttaat
      121 tcttccttta ttatgctctt attttttata tgtctattgt tttgtcagat atattactac
      181 ttttattttc tataaatata tggctataac cttcttacgc ataaactcta taaatttata
      241 tactttatat actttatatt ctttattacc caaagcctcc aaaatggctt aaatcttttc
      301 atagcttctt acagaagttt acatggtcat ccccccaata agcaaaagtg caatatgtgt
      361 accattgtgc cctacttgcg actattgagc tgtcgaaatt cgcaacttcg catttatctg
      421 atgaaatgca ctatacaaaa tattcataac atccacaaca gagttaaagt tacctattca
      481 tattcactgt aaaaatttct tccacaggaa atgtaatttt aaccaatcct attgagacaa
      541 actcaccact tatttttttg aactcacgca gtaatacatc acttgcccga aaaaaagcct
      601 cactttcatc atctccagta agtgctattg tgcaatgggg tacccaacga ttggggtgta
      661 ccattcccaa ccatttgaat caaaatcttg tagtacctca tataattctc tctgaaactg
      721 atacatactg ctattcatta taggtgatac aaacaccgtt ttagtatcat taaacatgcc
      781 tactgaacca atgtaagcag gtattacctt atggttttgt gcaaacccct ttagcttgtc
      841 tatgcaacaa gcctcatcca catcattgaa acaagccaaa gtaagatgag gtcttgtttt
      901 ccattctaag tacttcgtac taattttttc atcagcgatt tttttagata aatcaaaaag
      961 ttgcttttcc gtttttttat cataatatag ctctatcgca tattgcataa taacacctcc
     1021 accataaagt aaaaatacct atctataaaa acacacaaat acctatcatt ttaatattat
     1081 attactactg ttcctcttcc aattttctat atagtgtagc caaacctatg cctaagtctt
     1141 ttgctatctt cttcttcgtc tttgtatctc ttccatactt attcaacaac ttgtttatgt
     1201 aagtcaactc taattcttta attgaaagta tcctttcttg attctctact atccctccaa
     1261 ctatatcatc ttcaaatatt atatttacat ccttattttt ataaatattt ccattaatat
     1321 tataatagtt taaaatatct aatggaagca tatctttata tattattcca tcatctccaa
     1381 ccaaattcat catatattca actgcatttt ccagttccct tatatttccc ggccaattat
     1441 agttaatcaa catattcatt acattttctt ctattttatt taactgaata cctaattcta
     1501 aagaatattt agaagcaaac ttcatcatta ttgcctcaat atcatccttt ctttccctaa
     1561 gaggtggcaa atttatagga attacattca atctatagta taaatcatcc ctaaacttac
     1621 cttcatttac taatttcaat aagtttttat ttgtagcagc aatcactctt atatctaaat
     1681 ctataagttt atttgaccca attctcgcaa attttctctc ttgtaaaact ctaagtaact
     1741 ttgcttgtaa aggcattgac aaatctccta tttcatcaag aaatataaca ccttcatttg
     1801 caagttcaaa tttacctact ttacctccac tactagctcc actaaatgca ccttttatat
     1861 agccaaatag ctcactttca aggagatttt ctggaatagc agcacagttt atcgcaataa
     1921 atggcttgtt ccatctgcta ccttcagcat gtatagccct agcaattagc tctttacccg
     1981 taccactttc tccagttata agtacagttg aatttgattt tgctaatttc ttagttctct
     2041 cttttacttt taacattgct tctgaatttc caattataga ctctatatca ctatttcccc
     2101 agccactatt cacttttcca tgtcctttat gattgatata tgtcttgtca aatattatta
     2161 ttttgtcgta ttgatttata tatggatata ccggaattat tttggcattt atattataaa
     2221 ttttgttatc aatatcaatt tctagtaatt catgtccatt tgaggaagat tcaacagata
     2281 ctatatttac tatattttct attatatttt tatctatatc taattttttt agagctatat
     2341 tgtttatata agatattttg ctatttatgt ctagtattat aacgcctttt tcaacatttt
     2401 caatgatttc tatcatcata tccatttttc tactgctatt ttttgacgct tcatgttctt
     2461 ctattttcat agaaattaaa tcgcatatat gattgacaaa ttttaaataa ttatctattt
     2521 ttgcacttat ttctactttt ttagttttat ctgtactaat agcaccaatt actcctatag
     2581 ttcgattatt atattttatt ggaactgcta ttattacttt atttaggcat ttcatgtaat
     2641 gggaacattc tatacaaagc tgattatcac atatatccaa tacgactaat tcctgacctg
     2701 tctgtataac attgtcataa ataaatcctt ctgtaacaac gctttcattt atctttgatt
     2761 tatacagacc tgtaccacta attcttatta atctatcgtc aactatttca atatctacat
     2821 ctaacacact agctactgtt tcagtgtatt ttatcatttc ttgttgaaat tcttttaatt
     2881 tacttaacat agtttccccc attagaattt aatttaccaa attatcagtt tagctatatt
     2941 ttaacatata gtcactcttt aaaatatata taaaacatta cttatttata tatttgcgtt
     3001 aaaacagaca atattaattt ataaaaagtt tgttttatat agtaacctta attataatat
     3061 gcagacaata aaataaatca caatacaata ataaaaaaaa gctttcctca tataacaaat
     3121 atatttttga cataaaaata tacattatat gagaaaagct caatagttaa atatttagat
     3181 agatttttat atagactttt ttaatataaa cttttttaat tgtagacttt ttttattagt
     3241 ttttaatttt tattagtttt gttattggtt ttattgttat aatacttata atactgccat
     3301 tgcttcaatt tctaaaactg catcttttgg taattgagca acttgtacgc atgaccttgc
     3361 tggtggattt tctttaaaat atgtagcata tacttcatta atatcattaa aatcctttaa
     3421 atcttttacg aatactgtag tcttaaatat tttatccata cttgtaccag cttgttctaa
     3481 tattgctttt acattatcta aagaagcttt tgttgcttct tttgggtcag atactaactc
     3541 gccagtcttc atatctattg gaagttgacc tgatgtagaa actatgtttc caacttgaat
     3601 tgcttgagaa tatggtccaa ttgctcctgg agctttctct gtagctatta ctacttttga
     3661 catgacttca cctcctatta ttaagttata tttacaggaa aataaaattt cctaaacacc
     3721 aatattaata cgaaccttta gattaaattt caaatctaaa acttcaaata atcaatactt
     3781 agatgattat gtttatttgt gtcttgttaa cttttattaa agcaattaac gtgccaaact
     3841 ttatttatat ataaaatttt gtgaagtcat tcaaaatagt tacttctgaa ttttttcgta
     3901 aaaaaacgct ctaaaattac ttttttatga tgtcaatttt tttctcatat ctagaaattt
     3961 accaacatga gaattcttgt tttgagaatt tacagattaa agttattttt taaccttaaa
     4021 ttctgaacct cccatgacta aagtcacagg gttcctgctt catagaattt tgcataatca
     4081 agttattaca tatgaaagaa ctatagttga caatcccact attataaata ataaaacctt
     4141 tacccatacc ttatttaccc tagttatcat agagcctatt gaaaatataa cagctaaata
     4201 taataataac caaaaaatta tcaatatcta ccactgttgc tgaaaatcca aaaaactacc
     4261 ttttgatagt cttacttaac aatctaatta atctcataat aaatttacaa cttataaata
     4321 aattgttata ttggccgtat ctcaacaact ttctcctttc ctagttttaa atcttttata
     4381 gaattgtacc acgtctaagt aaattttcat actattaagt cttatttcta ataaatcatt
     4441 agctctcatt ctatgcccaa taatgtaaga ttgcagtaat tttattgatt ttcatagttt
     4501 tatttaatat acttacatta attattttca tatgaaatta ttatttatat agggggattt
     4561 ttttaatatg agcaaaagtg aattaacagc agaaacaaca gaagaaatgt tagaagtact
     4621 aagtggtaaa gattatgata ttgcatgtca tttacatgaa cttggtaaat cattagattg
     4681 taaaattgaa ccaaaaacag gtgctcgttc ttacaaaata gtatattcaa ctaagaaacc
     4741 aaaacgtagc ttatttacta ttgaatgtaa tgaaaaaaaa tggagagtta aagcaaatct
     4801 ttttcatcta aatacatata aagatgctgt ggaggaatgc tctaaaacta ttaaagatag
     4861 tataactaaa actcgtactt gtaagaaatg taattcaaag tgtattggag gttcttcgtt
     4921 tgaattagat ggaaagtctt acctgacttg cataggaagt ggtcattatt ttgcaaatat
     4981 ggaagaaatg gattggaaaa acctagaaaa attaattact aaagaaaata atattatgca
     5041 ggaatctgta tagtttttta agggtaatta ttaaaatata tgagtaacaa cagatactta
     5101 aataggcata aaaaaataaa gggtataata aggatataga ataaaaataa atgatatagc
     5161 acaaagaggc tgtctcaaaa tacagataag cctcttttta ttgttgaaat gataagaata
     5221 tattcaattt tatacaattt tatacaaaca tactacgcca acttacccat ttcatatgcc
     5281 tgtttcattg ccttacttcc cttaatatca cctacattcc aagcacctac accatatact
     5341 ataccttttt ccttagaacc atttagacat gatgtaaatc ctctaaatcc ttctattgtt
     5401 ctttccatat cagccacatt ttcatctgct gctgtaacga taaaatagaa atctttatca
     5461 caaatttcag tatatctaga gcaagttcta tcaatcatag ttttcatttg tgcattcata
     5521 gtataaaaat aaactggagt cgctaaaaca atcacatctg ccccaatcat tttttcaaga
     5581 atttctccca tgtcatctgt ctgtgaacaa tttgcttctt tttcataaca agcaccacat
     5641 ccagtacaat agttaatatc tttatctttt aaaaatatct tctccacttg atttttagat
     5701 tctaataccc cttgtataaa ctggtcacac aataaatcag aatttccacc ttttctagga
     5761 ctagctgata ctattaatac atttttactc ataagatact gcctccattt tcttatttct
     5821 actgctcttt aatacttttt ctttctcaat aataccttct tcatatctat caatcttaaa
     5881 atttaatcgt tccaaagttt tttgcatttc ttctattctt tttgctagtt ggtttctctg
     5941 ctctattaaa agttcttttc tagctttgat agtactgctc ccttgttgaa acatagctac
     6001 atactcaatt aatgtctcaa tagaaagacc tgcacctctc atacacttta tgaaattaac
     6061 ccactcacaa tcttcttctg taaaagacct aattccactt ttatttctat ttacaggtgg
     6121 tattaaacca attcgttcat aatatcgtaa agtatccgct gaaagctcgt attttttact
     6181 aacttctgaa atcgtcatac atatcttctc ctcttgatat tgtatagatt gaattatttc
     6241 tcaaaggttt ctgctacatt ctttaaagca tctataattt taatgtgttg aggacagctt
     6301 tcttcacatt gtttacattc aatacaatct gaggcctttc cataagtttt agtataatta
     6361 ttatagtaga ccatctgtgt tgaaaatcca gtattaagag cttgtttttc tgcattgtac
     6421 aacgcaaaat agtttggaat agcgatattt tttggacagc cttctacaca atactgacag
     6481 gcagtacaag gaattaaaat tgattcgttg ataatctcta ctgcttcatc tataatatta
     6541 tattcctctt gtacgaatgg tttgaaattt tgcatatatc ctgtattgtc taataattgt
     6601 tccatatttg acatcccact taagaccatc ataacattat ctttacttgc tgcaaaacgt
     6661 attgcccaag atgaaattga catatctgga ttgtagtcat tcaataattt ctctgctttt
     6721 tctggtatct ttgcaagtgt acctcctttt actggctcca taacaattac aggcttatta
     6781 tgcttctctg ccacctcgta gcattttcta gattgtatgc tttcattatc ccaatctaaa
     6841 taattaatct gtaattgcac gaagtctact tctggatgct cactcaagac tttatccaat
     6901 aattctgcac tatcatgaaa agaaaaacca atattcttta tttttccttc ttttttcttt
     6961 tcttcaataa acttaaagct atcaaatttt ttagctactt cgtaatgtga cacgcctata
     7021 ttgtgtaaaa gatagtagtc aaaatagtct acaccacatt tttctaattg ctcattaaaa
     7081 atggtttctt gctcttcttt tttctttaaa gccattaatg gaagttttgt agctatagta
     7141 aaactctctc ttttgtgtct ttttactaga gattctctaa gtgctatctc actttttccc
     7201 atgtgataca cataggccgt atcaaaatat gtaaatcccc tttctagaaa agtgtctacc
     7261 attttattta aatgttccat atcaatagtt gtaacatcat tttcatcttt tattggtaaa
     7321 cgcattaatc caaatcctaa tttttttgcc tccattgctt tacttccccc tttaaaactt
     7381 gtttgttttt tctctctact ttaactattc ttacaagtat ataattcttg tataattata
     7441 ctttactact tggagttaac ttcaagtcaa tagatttttt tatttttaag acaaatatta
     7501 taaaaataca gttctttaat attaatccat tacatgtttc tttcaccctc caattctcaa
     7561 acttcacaaa ctccaacatg taaagaaata tatactttaa gaaattcagt ccattaatat
     7621 tactacaaat actttttaga tatgtttaaa aagaaatttc acctaaaaga tttagaaaag
     7681 atttagaaat aggtcttgtg tctattttaa aatatataaa acagtggtaa agacttattt
     7741 ataagataaa aaatgaatag tgtataccct ttttaaaaag ttttttcctt tttatatttg
     7801 tgatacaatg ttaaatataa aaaggaaaat tttgtattaa agaaatctta tttgttgatt
     7861 ttgtaacttc aaactcagca tcaatttgat tttaagatta tattttatta agtatgctaa
     7921 ataacaaatt tttaaagatt gtgaggtaaa aatatgaata agaaaaattt agctatatta
     7981 atatcaggta tgatgttttt ttctagttct tcagcaattt ttgcagacaa tactacaaag
     8041 caagaaagat taataggtaa aacaagatat gaaacagcag tagaagtaag taaattggga
     8101 tgggttcaat caaaaacagc cattatagtt aatggtaatt ctattcaaag tgcgctttgt
     8161 gcaaatccat ttgctaagct aaagaatgca cctatacttt tagtaaacaa caacagtatt
     8221 gaaaattcaa ctaaggctga gcttaaaagg cttggtgtag ataatgtgta cattgtagat
     8281 agtggaaata gcatttcttc taaagtagaa aatgaaataa aatcacttaa cataaaaata
     8341 aataaaatag taggaaacaa tatatatgaa atgtctacaa atgtacttaa agaaatagat
     8401 aagataaaaa aggtagaaaa tgttgcagtt gtaaatggac taaagggact atctgatgca
     8461 gttagtatag cttctccagc agcaatacat aatatgccta taatattagt atcagatgaa
     8521 aacagcaatg atagtagtat aaatttctta aacaataggt ctataaaaaa atcttacata
     8581 gttggaggaa ataagatttt atcagacaat acagtaaaga aatatccaaa cagagaaaga
     8641 atcagtggtt cagatagaaa caatactaat gcaaaaatta ttgataaatt ttataaagat
     8701 aaaaaaataa ataatgtatt tgtagctaaa gatggtatta accaagaaaa tgcattagta
     8761 gattcattat caataggagt actagctgct aaagaaaatt ctcctataat gatatctaat
     8821 ggtagccttg taaatagtca aaaagaattt attaaaaata aagatataga tgaaataaca
     8881 caagtaggag gaggatataa tcaaaaagca tttgaagata ttttaaaact aaaaaatgtg
     8941 aacataagaa ataaaactaa aaaaatatct ttaatacagg ctaaagaaat attaaaacaa
     9001 gctcttccag gaagcaatat tttagtttgt tattatgatg aagatgataa tgaatatgac
     9061 tgcaaagtta taaaggataa tatcctatat gagatagaaa ttgatgccta ctctggtaaa
     9121 ataatagaaa ttgaaaaaga tgatgactta aatgataata atttaggtac acaaaatata
     9181 tcattggata aagctaaatc tatagcatta gatgccattc ctaatggtaa tttaatctat
     9241 tgtaaatatg atgctgaaga taacgaatat gaggctaaag taataaaaaa taagaaaaca
     9301 tatgaattag agataagtgc atttgatgga agaattgtag atattgacat ggacaatgat
     9361 tctgatgatg actttgatga taatgataag aataactcta atactaatga caacagttct
     9421 attattagtg ctgaaaaagc taaatcaata atgctagata aagtgcctgg tgggaaaata
     9481 gtaaaattta agtatgatga agatgacaaa gaatatgaag gtgaaataat aaaaggaaaa
     9541 actgaatatg agataacaat taatgcaatt actggtaaaa tattagaatt tgaaatagat
     9601 gattaataaa aaatatatat tctattaatt attacaagca ataaagccat aaatattgaa
     9661 attttcaata tttatggctt tattttattt ttttaattta aatccacatt atgccattat
     9721 atatatctat gcactcttgt aaaatgggtt atatttctcc agtatattaa aaaaagtgag
     9781 tgtctcaaaa taaatcttct aactcataaa gcactctcct ttgtattaaa tcaaatatat
     9841 tttcatcatt taaacaatta agaagagact tctctctatt ttgagacaac tccattttaa
     9901 tacactattt atacataaac atattttaaa caactaaaaa gcaactaata tcttaataaa
     9961 tatctcaata catatcttca taacacatat caccataatt acaagcagat aaagaactct
    10021 cctcatttaa atacgactca aattcatacc caaacagaat tgttggttct atatatattt
    10081 ccatatctgt atatctccat cttcttacct ttgcctctat aaccttgtaa aaatcttcta
    10141 aactataccc ttcttttaat cttttatcta taagtgcctt agttatactc aaatcagctt
    10201 taaaattttt tcctgattca ctatttaatt ttcttataat aatttcatgt atgctttctg
    10261 gatttttgta agtagaatct tcagtcaaat cttcatccga attttcaaag gtatttttat
    10321 ttaaattctt tttatttaaa ttatttttta ataattcttt tttctttgtc tcattttctg
    10381 tttcataccc agtatgaatt atcaaatcat aatctttgtc tatactctct tgatttatac
    10441 ggctgtatat gttggtatta ctgttcttaa acaactcatt tctatgttta tatttcattg
    10501 ttttttggtt attgttaata gttgcatcaa tgtatttctc tatatttttg aaaatatcac
    10561 cattctttgt tacaatttct gcattactac tttcagaaca aacataacta tatacagaac
    10621 aacaatttcc ctttactccc ctacttatta atctcaatat acctaaatct ataaattcat
    10681 ttacaagtct acttattgta gatatagata catcaaggtc agatgcagct ttccttactg
    10741 tcatcataaa ttgtcctctt ggcagagttc cttttaatct ttgtatattt tcttttctca
    10801 ttacatagtg attaaacctt attctattta catctctttt ttcattaaaa tataatactg
    10861 ctgaatttaa ttgataaaaa ctattactca tattcccact cctccaattt aaatagataa
    10921 tttgttgtaa agtttttaga ttttttaata acttattaaa tgattatttt tattaaaaag
    10981 tattctttga tattttcatt ttaaagtttt aagtataata agttaatagt gtagatttaa
    11041 cttttttatt taaacaataa acttaatatt atttttaata taaaaatttt attaaattta
    11101 gtatgtaatt ttttaatctt attttattcc atctatactc ttatgctaag taataacttt
    11161 gataatattt ccctaactta tgtttcaact ggtttatatt gcaatttatt taattacagc
    11221 ttatttgcat aataattcgt ccatatcata agttgtgtat ttatataaat acacaattaa
    11281 aaataaatca ctatggtata ctaattatta cgaagactat acttatatgc aaagacaaat
    11341 ataaggcatc ttatatcttt tatcaattat aaaaaattaa acgctatctt atttatcata
    11401 aattacaaaa acataaaatt ttattactat attgctataa attaattgat acacatttta
    11461 cacaataagg agggctatat atgacaaatg ctaaaagtaa aaaagaatat ctaaatcgta
    11521 tttgcaaagt gcaagactat atagaagaac atttacatga acctctttct cttgatgaac
    11581 tttctaatat agcaggtttt tcaaaatttc attttcatag aatcttcaaa gcaatagaaa
    11641 aagaaacttt ggcacaatat gtaaataggc taaaactaga aaatgcaact gcttttctta
    11701 ttcatagaac tgacatgact gttactgata ttgcacacta cttcggcttt actgattcag
    11761 cagttttctc gagagcattt aaaaattact ataaaataag ccctataaaa tatagaaaca
    11821 attatagcaa gaattgcaaa gaaccatata aaatttctca gtacaataga agcatatcaa
    11881 aacctaaatg taagaatact agagaggtta aaggtgaaat tgaaattcta gaacttgata
    11941 atatgaatac tatttacact agatatactg gttcatatgg taatctaacc agtactttcc
    12001 caaaactttt agaacggtta ttcacatatg caagtgagca gaatctactt ggacttgaaa
    12061 acacacaaat attagccatt tatcatgata atccacaatt tacacaggag catcaattaa
    12121 ggactagtat ttgcatgacc attccagata acattgttgt caaagagaat gacaatcttg
    12181 gcaatatgat aataccttct ggaaaatatc ttgtgggaca ttttgatatt tcaaaatctg
    12241 aatatagtga tgcgtgggat tttatgtata atgaatggct atcaaatggt aattataagc
    12301 tacgtgcctc cttccctttt gaagtatatc ttaacaaccc acacaatcac cctcaaaata
    12361 gacacttggt cgatatatat ttacctatag aaccttttta ataacctcac ctaatctatt
    12421 cttgtattaa ggattaatat atccctattt tacggtttga taaaattgat aaaatgaggt
    12481 gaaccaatga aagaaataaa aattaatgga gcaaagatac acaatcttaa aaatatagat
    12541 gtttctattc caaaagataa gcttgttgtt gcaacaggtg tcagtggctc aggtaagtct
    12601 agccttatgt ttgacattgt atttgaggaa gggcgtaaac aatatctgca atctttaggt
    12661 atacttgcag gtattgatag tgaagataag tttgataata ttcaaggttt agctcccact
    12721 atagctgttc aacaaaatat tattcgccag agcaatcctc gttcaacagt aggcacaaga
    12781 accaatattc tcaacatgct tacactattg tattcagtgg aaggtcaaat aatgtgtact
    12841 atgtgtgata ctcctgtaag cgataatcta atttgcaata attgtggata tgaggaagaa
    12901 cgattaagac caagttattt ctcatacaac tcttcagatg gaatgtgtat gaagtgctct
    12961 ggacatggtg catattttga gattaatatt gaaaaattag tccttgataa gcatgatacc
    13021 ctaaaacaag tcttagatag agcaaagatt acacctggat atttgagagt attttctaaa
    13081 aaattcaatg attatttaga catacaattc ttacaactac ctgaagaaat aagaaatgaa
    13141 gtactctatg gtcattatga aaatggtaaa aaaagttcct ctttatcaaa ggtatttcac
    13201 aatcgttaca aaaggggcga agatttaaat ggagtttata ctatgactgc ctgttcagat
    13261 tgcaatggtt ttaaaattgg tgaagaagca cgtagagtac ttttaaacgg aaaacatatt
    13321 ggagaacttg gaaaaatgac gattttagaa attgatgatt tccttaaact cttaataaat
    13381 caaggtaatt taaacacatt tggtacaaat cttgtaaatg atattttatc caaaactcaa
    13441 catttaatag aatcacgatt aggtcatcta tccttatacc gagaaatgtc aactttgagt
    13501 ggtggggaaa tgcaaagact atttttaaat tcacatctcg attcagagat ggactcatta
    13561 atttatgtgc ttgatgaacc aacagctgga ttgcatgaat cagaaaaaat agacatctta
    13621 aaatcactaa agaaacttaa agatttaggc aatacggtca tagtagttga gcatgataaa
    13681 aacacaattg aaatggcaga acatattatt gatattggtc caaaagctgg tgttgaaggt
    13741 ggtcaattag tatatcaagg agatttagaa ggtctacttc aatccgacaa atcacttact
    13801 ggacagtatc tatctggaaa atatccaatg cctaatagaa caagcaataa aaacattaca
    13861 catactgata aaataccttg tataactatt caaaatgcaa atacaaataa tcttaaaaat
    13921 gtaactgtat cattaccttt gggtgctatg gttggaatat ctggaaaatc aggtagtggc
    13981 aagagttcac ttatatcaga caccctactt cctcttctta gaagccaatt taataaccat
    14041 actatcaata atcaaataaa ttcttcagag agtgaaatag gtgaggaaga cgattatgca
    14101 gttgttgaaa ctattgcaga tagactgatt ggtactaaac atatttctgg atattcagag
    14161 atatctcaat ctcctatagg caaaaatatg aactcaactc ctgcttctta tattggtata
    14221 tgggataaga tacgtacact atttgcaggt cagccagaat cgttagaaca aggatttaca
    14281 gctggtcatt tctcatttaa ctccaaaggg gcttgtccta aatgtagtgg tagtggatat
    14341 gaaaaggtct ggttgggaaa taatctaagt atagaccata tatgcagtga atgtcaggga
    14401 aaaagattta atgatgaatc tttatctatt aaatataaaa ataaaaatat acatgatatt
    14461 ttaaatatga gtgtttctga atctgtacat ttcttcaaag atattccaaa tattgtttca
    14521 cacttaaatg tattagaaca aataggtatg gggtatataa aattaggcca acctacaccg
    14581 actttaagtg gtggtgaatc ccaaagaatc aaacttgcaa aagaaattgg taaaaaacga
    14641 aatggtaata tcctttatgt attggatgag ccaactactg gtctaagtct atatgatact
    14701 tctaagttga ttcaattatt agatgaattg gttgaaaatg gaaattctgt aattgttgtt
    14761 gaacatgata tagatgtatt aaatgtttgt gactggattg ttgagcttgg tccagaaggt
    14821 ggtgacaaag gaggttatat aattgcagaa ggttctccaa aaactctaaa agaaaatcca
    14881 aaatctataa cagggaggta tctttaatga ataaaaacaa ttgcttagac ttaatttttg
    14941 aaaaagtaga cccttcaagt gttggtatga gttcagaaaa gctttcacaa cttgaacctt
    15001 taataatctc acaatatagc aatataaatg gtataattat tgcaagaaaa ggaaaaattg
    15061 tctttgaaaa gtactataat ggatataatg ctgatgatac ttttcatgtt gcatcagtta
    15121 caaaaagtat tatatcagcc ttaataggta ttgcaataga taaaggatat ataaaagatg
    15181 ttgaccaaaa ggttgttgat ttttttcctg aatatacttg tagtccttct gaaatatgta
    15241 aaaaagcagt aactatacgt cacctactta caatgactgc accataccct tttaaaaata
    15301 tgactgagtc tttagaaaga ctttgcaaac aaaaagattg gataaaatat accttgaatc
    15361 aacttggtca aaaaggcgaa attggtactt tcaaatattc ttcagctgga acgcacctac
    15421 tttcagctat tataacaaaa actactggaa aatctgctcg tgaatttgct aatgagtact
    15481 tatttaaacc tattggtatg aaagttatac cagattatga tatgaaagat tttggttttg
    15541 aagatttctt tggaaaaaat cttaagggtt gggcaaaaga tagacagggt tattctatag
    15601 gtggctgggg acttacattg agtgctagag atatgttgcg atttggtttt ctgtatttaa
    15661 atgatggtat ttggaataat aaacgtatta tatctaaatc gtggattgaa gaatcagtta
    15721 aaatgaatag taatgaatac ggttatctgt ggtggctatg taaaaaagat ggtgttttgt
    15781 ggtattcagc aatgggaact ggaggaaatg ctatttgttg tattccacaa tatgacttag
    15841 taatagccat accatctaaa atagtgttta aacctcgtga tagatgggag ttcattgaaa
    15901 aatatatact tacttctata atagattaat tactctttca taaactaatt actctttcat
    15961 aaactaatta ctctttcata aactaattac tcttagatag ccaaaccctc cattataaca
    16021 ccagactttt gtttggtgtt attatgacta atactatata tctaaaagga gttgaatact
    16081 aatggacact tacacgacat cagaggtagc agatttaatt ggtgtacatc ctaatacagt
    16141 acgtttatat gaagaactaa acttaatacc taaacctaag cgtaatacca atggttatcg
    16201 tatctttaat gattttcata tagaacaatt tagatttgct cgtacagcct tgaaaattga
    16261 agtggtacaa aggggattaa gaaaagatgc ccttgagatt gtaagaacat cagcgaatag
    16321 ggactttgat aaggctatta gtcttgtaaa tcaatacttg caacaattag aaatagaaaa
    16381 atctaatgcc gaggaagcac ttactattgt tgcacagatt ttaaatggaa atactccaga
    16441 aacaaataaa ctctttttaa ctagaaaaga aactgcagaa cacttaaacg ttacaataga
    16501 tactttaaga aattgggaaa tgaatggttt gttaaaagta aaaagaaaac aaaatggtta
    16561 tcgtgtatat actgatgaag atataaaacg acttaaaatt ataagcgcac ttcgttgtgc
    16621 aaactattct ctttcagcta ttttacgaat gttaaatgct ttatcttcta gtcctaatac
    16681 taatattaaa actgtccttg acactccaaa agatgatgac gaaattatct cagtgtgtga
    16741 caaactgata acttcattga aagaagctga acataatgca aaaaccatga aatctcatct
    16801 tcaaattatg aaaaaacaat tttcctagcc ctccactcta actccagact ttgatttgtg
    16861 tgttatcctt aagagcaaga aaaattcaac acacaaatca aaggaggaat aggatggaaa
    16921 acaaaaatac taattctaca tggaagaaaa aagtcattct ttttttagca agtcaatata
    16981 tttccttatt tggctcatca cttgtacaaa tggcaatgat atggtatgtc acacttcaga
    17041 catcatcagg tgtatgggta acagttttaa ctgtatgctc ctttatacct caaatgataa
    17101 tttctttttt tgctggtgta tgggcagact actacaatcg aaaggtactc ataattgtat
    17161 cagattcaac aattgctata tctacattag ttctaattct atttatgatg tcatcaaatg
    17221 gcacaacaag tgaattaata gccatatcag taatatctgt tatacgttca ctaggaagtg
    17281 gtgtacaaac acctgctgta aacgcaatga ttcctcaact tgtaccagaa gaacatttaa
    17341 tgcgttttaa tggtataaat agtagtctac agtctatagt acaatttata gttcctattg
    17401 tagctgctgc aatacttgga tttggtaata aaataaatca agttctttta attgatgtat
    17461 ttactgcaat tattgctatt gtattattaa tattcatcaa aattcctaaa catgatatac
    17521 caaaacaaac tgaaaaagtc tcattttttg cagatatggt atcagggata aagtactgtt
    17581 tttcagataa gttcattaga aaagtgcttt ttacttatgg agcattcatg cttctttgtg
    17641 ttccatcagg atttcttata ggacttctta ttgaacgaac atttggcaag ggttatatat
    17701 acctgtctat tattgaaatg attggatttt taggtatggt tttaggtgga ttgattatag
    17761 gagtttttgg tggttttaaa aatagaaata agaccttttt tctagcaata ttatcctatg
    17821 ctattttttc aattattcta ggtcttgtaa cacaagcttg gcaatttttt atatttatgt
    17881 tttttacgaa tttttcaatt ccaatcattc aagcatcagc tatgactatg ttgcaagagc
    17941 atgtagcacc acaaatgcta ggtagagttc ttagcttacc tattattata tatactggtt
    18001 ttattcctct tggtatgatg attttcggac caatggcaga tgtaatttct attaataatt
    18061 taataatcat agcagggatt atacttttat tttttgcttt ggctatcccc tattcaaaac
    18121 aattttacaa gcaaggaata tctaagtcaa atgaatccga gtaatatatt taaatagttt
    18181 aactcgattt atctggttaa aaatgaaggg gcttaaaatg gactttttaa tttatgaaga
    18241 aaatttttta tatgaaatca aatatatttt atcatttcaa cattgaaaaa taggtttatc
    18301 tttattttga gtccctctct ttatagcaaa taatattttg atactatctt tattaacttt
    18361 atataataat acctttactt gtaggttata atgatgaaat ttatcaaatc atataataat
    18421 acctccattt atatcataaa ttaaatttat agtacaaatg gaggaaaata aagtatttat
    18481 atagtttaag tacctattaa gttttccact aagtagctct aattgtactg aacttcttac
    18541 taaattatat tgaataatta taaaataaaa ctaactatta cactacataa attaaagtat
    18601 acaaattgca ctacataaat tacactatac aaagtatatt aaatcaccta ttaaattgtc
    18661 ccaattataa ttatactgag ttatctacta aaagataact ttcttttttt atctaaaata
    18721 atatatatca taaaccatac tacacaatgt acagccaaaa ttacaatatc tttgataata
    18781 atgacattac cattaataat ctccataata ccattaaatg tagcaactga aggcattata
    18841 tagcataaat tccctatacc actggtatta acccctagaa gatatattaa caaaggagta
    18901 cctataaata aaatcattac tattttaata taaacaattc ctaccataag accttctgac
    18961 aactttccaa taaataaacc tataagtgat gaaataaatg ctgaaaatac aacgagtaac
    19021 aacatcatta tcatatctaa tattgaaata tccaaacata taaatacagt tatcattgaa
    19081 gataaacacc cacataaaag acccaaaaat actttttgta taatatactg acttctactc
    19141 tgtggcaaaa cttcatttat aaatgatatt ccatcttctt tttccgatat aatattcatt
    19201 gcattaaagg tgcatcccat aaacatagca gtaattaaaa tcatagcaat aaaaacattt
    19261 tgaaactctt ttaaaatact atctttaggc aatattgtaa cctcaacatc ttttaaatca
    19321 ccctttagtc taaacatctc tggcaatgtc tttccaacat tttttatagc attaatctca
    19381 tttccataaa acacagtttt tattccttta ccatctctaa caactccaat tgtgtccgtt
    19441 ttaatatcct taatttcacg tattaaatgc atttttgtgt catatacact taccatacca
    19501 tacttcttca accactgctc tgtttgtaca ggaagtgtat ccttcactat accaaaacgt
    19561 atctctccta atgaagatat atcaattgtt cccataaaat ttattaaaat cgccataaca
    19621 actggtaaaa agaaggaaat tatacacatt ttatcttttt ttatactctt taactggtaa
    19681 cgtaatccta tcaagattat ccctccctaa ccaattcttt attaaaaata tattttacaa
    19741 gtaaaaatag aactattatt gtcaaagaac acactacaaa ataaactaat tcttttgaaa
    19801 aattcatgaa aaacgcattt ttaagctgac tataaacatg atagaaggga tgatatttaa
    19861 tccaatctat ttcaaaagat gtatttgcag atagaaatat aggtgtagtt atgaatacag
    19921 caaccacaag atatataaga gaaaattgct taaaatctct caataacatt gagcatccta
    19981 ttccaatcaa agacataata attgataaaa tactagcttg aactaatact tgtggaagta
    20041 tttttattcc ttctaaaaat ccaatattaa atactactaa aagaactgta aaacataggt
    20101 cacatataaa aaaagcaaat atctttgata atataaaaag acttcttttt acaggtaaaa
    20161 taccagtaat acgcattact cccatctgct tttctttaaa taataaagag gcaatcccta
    20221 aaaatccaat agctacaatt tcaaagaata aaatctcaca agtcatgtct tttctttgtt
    20281 ttatctcaga tgtatcctta tcattaattt gaattattgc attgttgtct gatattttgc
    20341 tattatggtc tttttctgat gataataatg acaatgcata gtcagctcta taattgtcaa
    20401 ctcctttaat actttcatgt aaaattactt tcggcttact ggaactcata tcaatacaaa
    20461 ctgcacttgt atcttcatca agttttgctt ttaattcacc tatagaagaa acttgattaa
    20521 ttaatggaga tgcactcatg cttatattct ctggattata tagatataca ttattgtgat
    20581 aactatcttc atccataaac tttacataac caaaattaat ataacacgaa taaagtatga
    20641 gagagccaag tgtcagcaca aaaaatttac cataaatcat catccaaaaa tcttttttta
    20701 ctaaggtaaa aaatttagaa tacattacac taacatcctt cctgtgtatt taataaaaac
    20761 ttcttctagt gtaggttctt ttgaatgtac acttaagatt tcatcatatt taaaagttat
    20821 tccagattct aactcttgta tatctacaat ttcaatatct cgatttccat tatatacaaa
    20881 ttccacttca actttattat tgctattttt catttttaaa tttttgggtt tatctattac
    20941 cataatcttt ccatctgcaa taaaagccac attgttacac attaaatctg catctagcat
    21001 attatgagta gtcaaaaaga tagttgttcc tttttttcgt tcttcttcaa taatcttacg
    21061 aaatagtaca gctccagatg ggtcaagtcc actagtaggc tcatctaaaa ataacaactt
    21121 tggattactt accaatgctc ttgccatgct aatacgttgc ttcatacctt ttgagtagga
    21181 tgatacaggc ttatttataa actctttctt aaactctaac aaatctaaga gttcatatat
    21241 gtctctcctt tgatttttag gataaaaaga cgaaaaatat tctaaattat caattgcact
    21301 taaattggta tataaataag gaaattcaaa taaaacacca atcttttgat ataaagattc
    21361 tgtgtatgct ttaacttcct taccaaaaat ggatacgtga ccattgtaac cttttaaaac
    21421 accatttaag attttctgtg ttgtggattt tcctgaacca ttaggtccaa ggaatccaaa
    21481 gatttctcct tcttttactt caaaattaag tccatgcaat atctgtttat cttttccata
    21541 agaaaatttt aactctctta cttttatcat ctatcataac ctcctttatc atttttttgt
    21601 cgtctatccc accattttat aaatttaatt ttaagcggaa tagaaagcaa tattatcaat
    21661 ataatagtca accatgtata ccatttcata agtactcccc cttgtaacct tatttaatta
    21721 cgcttctatt ttttaattta tttaacctat tttatttcta tttggtgaaa tcagagtgtc
    21781 tctcttgtga aatcgaagtg aaataataaa aagcttaata aaaaatcttg ttttcatcag
    21841 gaaaacaaga ctttttatta agcttttcat taagcttttt attagacttt ttcattaagc
    21901 tttttcatta agttttttta ttttattctc tttaaatact tacgtataca aataaaaata
    21961 ttttacatca gatacttata atttataaat taatactaaa ttattcatca attggaatca
    22021 taaaataaaa ctctactcct ctatcacgat tattcactcc atattctaac ccttgcattg
    22081 taagtatttg tgaaacaatt gcaagcccta gacctgaacc tcctgtacgc tgagattgaa
    22141 cactacgata aaacttagac caaatctttg aaatctcatc ttgctctata aacctaccat
    22201 cattataaat ttcaaataaa agcaagccat tttcacatgt aatcgataaa tctaaattac
    22261 cattatatac aacatgcttc tttgcattaa taactagatt ctctactact tgttccattc
    22321 tcattttatc tgccactaca aaaacttcat actctggcaa atcatatgta aaataaaaat
    22381 tggcatctgg tatatcaatt aaaagacgac ctgccacagt ttcaacaaac tctataaaat
    22441 caaaacgctc tgggttaagt ttagaaactc ctgcttccaa tgcagataag tctaataatg
    22501 atacaatcat attgttcatt ctatcagttt cttctattat aacattcata taatttttct
    22561 ttttttcttc atcaacttca tctataagcc cctctgtata cgcacgtata atgccaagtg
    22621 gagtcttcat ttcatgtgaa attgtatcta ccaactcctt tctttgttcc aatagcattc
    22681 tttccttatt tacatccttt tcaagttgaa tatttgcact ttctaggtct gataatgcct
    22741 gttgtagatt tgtagacatt gtattcaggc tcctagaaag ttctccaaat tcatcattag
    22801 tatttacagt acatggatta gaaaaatcaa gttgggacat actttttgca gatttattta
    22861 tagatgtgat tggttctgaa ataaattttc ccaataagaa atcaatactc ataataaata
    22921 taaccagtat tataaaccaa atccaaaatt caaatttaga gtttatcgaa aattcttcaa
    22981 caaccagata aaataatata attaacgctc ctgataattt agaaagtaaa aacacttttt
    23041 ttctaactga atatagtcca aaattttgtt taatccaatt tgaaatatct acatttttca
    23101 gtttttctat aatctttctt aaactattca ttccttaacc tcaaacttat atcctgaacg
    23161 aattaatgta actatatggt gtccttcact accaagcttt ttgcgtaagg tttttatatg
    23221 tgtatctaca gttcgtgtgt ttccttcaaa atcatatccc catatacaat ttaaaagttt
    23281 ctctctggaa aaaacttgat tagggttttc cataaataaa cgcaataatt caaattcttt
    23341 atatgttaaa tcaatcaatt tatcttctac atacacttgt cttgatgcta ccataataaa
    23401 aatctttcct aaactcaatg aatttttggg aatatcagta gtgtatctac gtaacaaagc
    23461 attaactttt gctaaaagta cttttgggct aaatggtttt gttatataat catctgcgcc
    23521 atattcataa ccctttaatt tatcctcctc ttcaccttta gctgtcaata taataatagg
    23581 tatattagtc atttcacgtg aaactctaca cactgaaaat ccatctaaat acggcatcat
    23641 aatatccaat atcattaaat ctatattgtt gctctttaat attgtaagtg cctcgactcc
    23701 atcatcagca gttatacaag tatatcctcc cttttgcata tattctacaa taatattttg
    23761 cattcttttt tcatcttcta caactagtat tttagccatt tctatttatc acctcttttt
    23821 tgtaggcttt attttagttt atattaattt atttattttt caactatttt tttatagtat
    23881 tttttatagc attttgatag tattttttag actattctta atggaattga tatcatagat
    23941 aaaaatagcc tactaacttt ttctatgtta gcaggctaaa atatttctaa taataattat
    24001 attattttgt cactattcta ttttttcgat taaaatatat tctaaagcaa atatcttcaa
    24061 ttaatgaata aattgcactt gttataaagt acagacctat ggctactgga gttctaacag
    24121 ttagtataag acttgttatg gtagtcacca cagccatttg tttgttaaac tttacttgag
    24181 aatttatttt taaatatggt atataattca ctaaatttga agctaacata attaatgtat
    24241 atatacaagg taatataaac aaattatcaa atgtacctaa attagcaatc catggaatta
    24301 taattgttgt aaattccttt gtcatatcta aacatacatg atataaagca tatactactg
    24361 gcatttgaac caaaactata agacatccca gcatactctt catactttct acagaactta
    24421 cttgtatttg cttttccaat tctttagtat cattcttata cttttctttt atatattcca
    24481 ttttttcagc taattcttgc tgttttttta cagaaaatct ttgttttaat gatagtggca
    24541 taagtactaa tttaactatt aaagttataa gtacaataga aattccaaaa tcacctgtaa
    24601 aattaaataa tatacttaat aatccttgca atatacttga aatgaaatcc attttattct
    24661 ctccttttta tttttaaact ttagaattta taatagattc atttcaaatt aaaacaaact
    24721 acaatacctc caaatatcat atttctgaaa aactatatac tgtttaggca gtcgttcttc
    24781 agaatatgta attctattta caaatatgtc tcttattaaa acatacttaa ttttatgatt
    24841 taagaggtct ttacaaaaat aaaatatact tccaatgata gataccaata atgtcatata
    24901 aattagtaca tataacatac tatccatatc taatctcatc acatcacctc ttcatcactt
    24961 gtatttattt ttagacaatt tcaagtccta aataattcca acatttatat ttagttttaa
    25021 ataattatag tatagaaaac t
//
LOCUS       DAGSRM010000049        24175 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.049, whole genome shotgun sequence.
ACCESSION   DAGSRM010000049 DAGSRM010000000
VERSION     DAGSRM010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24175
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.049"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            <1..1115
                     /locus_tag="MJE33_002188"
     CDS             <1..1115
                     /locus_tag="MJE33_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBY9648120.1"
                     /translation="FVKYIIKQSTVLSIVSTLLVYLIEQLVYGFTAPLNYLIFTSDIN
                     TDIYKFDINMGLSSLITIIIAGFIYWYSSKKFNIKVMNFDKYIAILMIPLLLIILFMQ
                     SFEYSSNINIDTSLGIVKLLLNTSITEEIQAFLFSIIGTICVFFSLFTFKKLIQALED
                     DKERAIMNQQIHAQKNYIEEAKSRLSQTISFRHDFNNHLAIVNGLLKKDQILKAQDYL
                     NKLEKVTSDLSFSCNTQNIVIDALFNNKLSIAKQLGIEVDCEVIIPQSTNMNDFDLCI
                     VFANAIDNAIKACRVVDKQNRYLKLSTKLEGGFFMIEIKNSYNPINNYTSGSGIGLLN
                     IKEIAKKYQGAISIDKTSDYFQINVLLVISLHCASI"
     gene            1154..1639
                     /locus_tag="MJE33_002189"
     CDS             1154..1639
                     /locus_tag="MJE33_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648121.1"
                     /translation="MYLNRESLIYRNEEVTKMKVGNINGQAPITNGLDSCQSEVQGLE
                     QKKKSLQDEIEKVRSDKSIDGETAKLKIEALKKEMEEIENKIQELKGNKAKKTNKEEN
                     NVSEVNNNLLERKKEPSNNENVKQLYDVFERSNVDEQWDNTYRVLQDDKNLKVKFNRP
                     I"
     gene            complement(1902..2138)
                     /locus_tag="MJE33_002190"
     CDS             complement(1902..2138)
                     /locus_tag="MJE33_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648122.1"
                     /translation="MLLTHIYFNGQFKQTIELYKQAFNATVKTLIEDSCYLLIVHNEI
                     VIHNKFLILNDFGNKNGVSISEGYQPTDKIQTAS"
     gene            complement(2293..2517)
                     /locus_tag="MJE33_002191"
     CDS             complement(2293..2517)
                     /locus_tag="MJE33_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBY9648123.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
     gene            complement(2614..3867)
                     /locus_tag="MJE33_002192"
     CDS             complement(2614..3867)
                     /locus_tag="MJE33_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HBY9648124.1"
                     /translation="MITLSFDIVQTLTLSIFFFLIGNLLKNKVNFLNNFCIPAPVIGG
                     LLFCFLNLFFKYFNIADISISGNLMPNFITFFFTTIGLEISINLIKKGGSVLFRYWIL
                     CGVLAFCQNILAITISKIIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVG
                     VGLSAATIGLIMGGILGCPVAKFLINKYKLKPSSNIDLSIPRYNRNLKRFGSKFFRNK
                     NRIKNNNFSETITPSVFLEQVLLIFICINTGEIISRCFYITFNILLPSVVTCMFSAVI
                     FRNLNDKINILELNFKLIDFLKELSLGIFLTLSLMNIDLFELSTLLPPILLIVTFQVI
                     FIILFSIFICFRVLGKDFDSAIIISGLIGHGIGATPNALANMSSLTQKYGDSPKAFLV
                     VPLVSGFLLDAISIPCILFFINILT"
     gene            4127..5116
                     /locus_tag="MJE33_002193"
     CDS             4127..5116
                     /locus_tag="MJE33_002193"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBY9648125.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDVFKLAREYGYKVTIHAGEAASGENVLDAINILKADRI
                     GHGVKIKDHKKAYNLVKDKKILLELCPTSNVQTKTVDSYEAHPFYTFYKDNLHVSINT
                     DNRTVSDINLSSELNVIFDTFKLGLEDYKIIYRNAVEASFADKETKEYLNSLI"
     gene            5188..5928
                     /locus_tag="MJE33_002194"
     CDS             5188..5928
                     /locus_tag="MJE33_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HBY9648126.1"
                     /translation="MKDYEDVVYIKLVKKELEEWKKNILKKPSIFDKASKGIQNKFNG
                     VLPDKYHEIITSSIKVMTKTVLFGYKYATKPPLRNMSLEEREKLVLEKAKTYKTTAMI
                     EGAGTGAGGILLGLSDFPLLLGIKVKFLYDVASIYGYDVKDYRERIYILNILQLAFSS
                     QQHINKTFKLMNNWDEYVNSLPEDINDFDWRTFQQEYRDYLDLAKFLQLMPGIGAFVG
                     FYVNGKLIDKLCETAVSAYRLRIRELKL"
     gene            6144..7295
                     /locus_tag="MJE33_002195"
     CDS             6144..7295
                     /locus_tag="MJE33_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBY9648127.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRTAIKDKNEERELLKEFYALGKLQPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVQKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            7320..8075
                     /locus_tag="MJE33_002196"
     CDS             7320..8075
                     /locus_tag="MJE33_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HBY9648128.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKSTSKQVYE
                     LLSSEIDKKDIKKVVGTGYGRGVMDFADKKVTEITCHTRGIYFLNKNIRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAKGYEPVNISSM
                     CTVFAESEIVSLLAQNISTGEVAAGILKSIANKSTSMLARGEVIDEVAFTGGLAKSKE
                     LVKMIEEILGKKIFIAEDTQIIGALGAAVIGFR"
     gene            8267..10654
                     /locus_tag="MJE33_002197"
     CDS             8267..10654
                     /locus_tag="MJE33_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HBY9648129.1"
                     /translation="MKKFTSKKVTRFVALFLISILVMSTIPVSADNSNTLNQIKKAEY
                     SETYKQYLNDAKNGCTEKYNGIIPNPYQLEGTQIQSKGRTAPTSYDPRKLGLMTSIKN
                     QEDLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVNDMNGALNYE
                     AMGYLTSWSGPKLEKDIPYNGRVSKAQGAKKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSGYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKDNFNANI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGKKPNDDEKMYQHD
                     YASIVPLISKKITAANVFDFNRGDETLKSVMFLTESIGAKYEVYYAPVVNGIPQENNM
                     KKLKEGTAQYSGYITVPIDSFDIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSNFYPCNFTIKAVTEKSSGESIPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGSSIVDALAATPFTSAINSPILLTQKEALNSKTKAEIQRLG
                     AKKVYLIGGENSISKEIEQQLKSLNISIERISGSDRYKTSLLLAQKLNGIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSKIIDNFYKKDTLKNAFVVKNGIKNQNDLIDGLAVG
                     PLGAKTGSPVILVGDKLADSQKEVLKNKTLEKVTQVGGGANKNAFKELVKLKSAK"
     gene            complement(10885..11118)
                     /locus_tag="MJE33_002198"
     CDS             complement(10885..11118)
                     /locus_tag="MJE33_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBY9648130.1"
                     /translation="MATTTKNRFYDYVQENLNKYVEVHSYFSSCPLYGEIIEADSLSI
                     TLCSRYIDESNPTLNETYTLYLPLSSIISIRTL"
     gene            11407..11997
                     /locus_tag="MJE33_002199"
     CDS             11407..11997
                     /locus_tag="MJE33_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY9648131.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMEQYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRVDESITCEQLTDIFMKFYRMV
                     DSTSILNLMINGEFEILVRKLPDSIIEEYFRHDDFEIGEIISCIPNAKNKDIESFSGA
                     FRAVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
     gene            12111..12956
                     /locus_tag="MJE33_002200"
     CDS             12111..12956
                     /locus_tag="MJE33_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9648132.1"
                     /translation="MIKVDDLSFSYTDRDFLQNINFEVGKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYGGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDELLKSVGLENESNKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKSKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVRYTYFDNGEKTSECFLNNTANDKNLNMLIEKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            12953..13636
                     /locus_tag="MJE33_002201"
     CDS             12953..13636
                     /locus_tag="MJE33_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9648133.1"
                     /translation="MRLKNLILGDIKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IAIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMFIAITLNLDNIIISTIGTFFSSIIFSLLGLIIASKASSLNQFIVLSIPIEIICFI
                     PPILNVLLDTKSYANLYPFNICISLISGDKNFIMINILILISIIIIIYFITYYFICSS
                     WKKVGGVKL"
     gene            13633..14349
                     /locus_tag="MJE33_002202"
     CDS             13633..14349
                     /locus_tag="MJE33_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9648134.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISRYLIVTPLGK
                     TGYLISRFGIPGILAFIITMILLIFFSLTKISLLLNLSISLLVLLQGVIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKAQYILFFLPTFWISKAFKEDNYVYMFISI
                     VISLIWILLLFKKFSKKIVK"
     gene            14446..14709
                     /locus_tag="MJE33_002203"
     CDS             14446..14709
                     /locus_tag="MJE33_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY9648135.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            14681..15136
                     /gene="def"
                     /locus_tag="MJE33_002204"
     CDS             14681..15136
                     /gene="def"
                     /locus_tag="MJE33_002204"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBY9648136.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIEQEGEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            15188..15649
                     /locus_tag="MJE33_002205"
     CDS             15188..15649
                     /locus_tag="MJE33_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HBY9648137.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHLLVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENL
                     YDDWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKDYELWSGNIMF"
     gene            15699..16601
                     /locus_tag="MJE33_002206"
     CDS             15699..16601
                     /locus_tag="MJE33_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBY9648138.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLP
                     GTLYLRGITRGVFFDFVKKEFPELFDKLSILYSAGSLNREYKNQLYKMVNELRYKYSL
                     SSSYSKIMKEKLKNSGDVQISFFD"
     gene            16971..17783
                     /locus_tag="MJE33_002207"
     CDS             16971..17783
                     /locus_tag="MJE33_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBY9648139.1"
                     /translation="MYLERPELYKQSEINFWDDEYISKQLLKAHLDTNFEGASRNFNF
                     IEDSVNWIVTVANPANYPKLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSINYAQNRN
                     KETNLNINYLFQSYLNMNYNEEFDLATLIYCDYGALSTENRRLLMEKIYDSLKPGGKL
                     ILDVFTINKYNNFEEIKYWEINEDGGFWSNEKYMCLQDNCKYNDYNTLEQTLVITEKD
                     ENVFYVWNHYFSKESFLLEVENIGFKSVEFFSNVKGEAYSDDSMTMGLVLQK"
     gene            18831..19592
                     /locus_tag="MJE33_002208"
     CDS             18831..19592
                     /locus_tag="MJE33_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBY9648140.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQHINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWNGKRVFIIATMALFSGDGSGVLSRILKKY
                     GAITVGGLHVKMPDSIGDEKVLKHSLEYNRKLVIKAEKKVTKAAKKLKHGSPPKEGLG
                     FLSHIVGLFGQRLYFINKTNMYTDKLKINTEECIVCGKCVNLCPMKNLVIKNCMIVAN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            19657..20439
                     /locus_tag="MJE33_002209"
     CDS             19657..20439
                     /locus_tag="MJE33_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBY9648141.1"
                     /translation="MEYYIVDSFATKLFKGNPAGVCVLDRRIPLELMQKIAEENNLPE
                     TAFVVKGKGNYELRWFTPKAEIDLCGHATLAAAYVISNFIDVNVKRIDFFTQSGKLEV
                     TRNGNLYEMIFPEIMPIEIELSPQQANLIGCVPSAVYSSRDLILLLNSEQEVINYKPN
                     YAQLRKLTDWLGIIITAQGSNTDFVSRYFCPELDSEDPVTGSSHCNLIPYWSEKLGKH
                     KMVAAQLSNRGGIIQCEVLKDNTVKISGEAVLFMQGTIKIDI"
     gene            20706..21125
                     /locus_tag="MJE33_002210"
     CDS             20706..21125
                     /locus_tag="MJE33_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HBY9648142.1"
                     /translation="MDKISGKLTVLFEEPFWVGIFERQDGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDNIGYKRMQRKVKKEQEKETIGTKAQNALKLQY
                     EERKQDRKNLAKNRKEEEKERLFNLKQEKRKAKHKGH"
     gene            complement(21228..21494)
                     /locus_tag="MJE33_002211"
     CDS             complement(21228..21494)
                     /locus_tag="MJE33_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBY9648143.1"
                     /translation="MKNFILIYFIVINSIAFFSMYIDKKRAIRNEWRIKEATLMSIAV
                     IGGSIGSIIGMYSFRHKTKHIKFTFGIPFILFLQFLLVYFYILK"
     gene            21721..22422
                     /locus_tag="MJE33_002212"
     CDS             21721..22422
                     /locus_tag="MJE33_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648144.1"
                     /translation="MKFIVEKEVFDKLENVCFGVVVAKGIDNTKEIERISNLLDISID
                     RVEDYFKDKKVKESEEIIPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDMDFRNDDVYVRFSKKGDKFVPFGETDVELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            22504..23112
                     /locus_tag="MJE33_002213"
     CDS             22504..23112
                     /locus_tag="MJE33_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBY9648145.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSSE
                     QLKNIYLKNEEDSVDAVVIVGLHKSSKKLILIRQFRVPINGYIYELPAGLVDEGESID
                     ISVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDEFLEPDE
                     EIEALLISQEDAKEILQSNHKIDTKAFLILQMFVSLGAKLFE"
     gene            23436..23900
                     /locus_tag="MJE33_002214"
     CDS             23436..23900
                     /locus_tag="MJE33_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 domain-containing lipoprotein"
                     /protein_id="HBY9648146.1"
                     /translation="MKKLVIILISIISMVSLTACKKSNESKYNNLNNKESGQTTIEMD
                     LDKNYDTSDPFVNARLFCVSNDIDVLDTEISFKMDGDSGIVEIKDNKTDETLWSNTWH
                     GSVDNDTFTISLSNIQKEKEYAICFTGIKINHAVVKVTFESNLVKEKEKPSK"
BASE COUNT         9717 a         2438 c         3946 g         8074 t
ORIGIN      
        1 tatttgtaaa gtacataata aaacaatcta ctgttttatc tatagtatca actttgttag
       61 tatatttaat tgaacaatta gtatatggat ttacagctcc attaaattac ttgatattta
      121 ctagtgatat aaatacagat atttataaat ttgatataaa tatgggacta tcttctttaa
      181 ttactattat aatagctgga tttatatatt ggtatagttc taaaaagttt aatattaagg
      241 tcatgaactt tgataaatat atagcaattt tgatgattcc attgttgctt ataatattat
      301 ttatgcaatc atttgaatat agttcaaata taaatataga tacaagtctt gggattgtaa
      361 agctgctatt aaatacttct ataactgaag aaattcaagc ttttcttttt tctataattg
      421 gaactatatg tgtatttttt tctctattta cctttaagaa actaattcaa gctctcgaag
      481 atgacaagga gagggcaatt atgaatcaac agattcatgc tcaaaaaaac tatattgaag
      541 aagcgaaatc acgattatca caaactatat cctttagaca tgattttaat aatcatttag
      601 ctattgtcaa tggattgttg aaaaaagacc aaattttaaa agcacaagat tatttaaata
      661 agcttgaaaa agtaacaagt gacctatcat tttcttgtaa tacacagaat attgttattg
      721 atgctctttt caacaataaa ttaagtatag caaaacaatt aggcatagag gtagattgtg
      781 aagtaattat accccaaagc actaacatga acgattttga cctatgtata gtgtttgcta
      841 atgctattga caatgcaatt aaagcttgta gagttgtaga taaacaaaat aggtatttaa
      901 agctttccac taagttagag ggtggatttt ttatgataga aataaaaaat agctataatc
      961 caataaataa ttatacaagt ggctcaggta taggtctttt aaatataaaa gaaattgcta
     1021 aaaaatatca aggggctata agtatagata agacatcaga ttattttcaa ataaatgtat
     1081 tgctggttat ttcattacat tgcgctagta tttgataaca atttcattga aaatagtatt
     1141 tgtaagctat attatgtact tgaatagaga aagtttgata tatagaaatg aggaggttac
     1201 taaaatgaaa gttggaaata taaatggtca agcaccaatt acaaatggtt tagatagttg
     1261 tcaaagtgag gtacaaggct tagaacaaaa gaaaaaatca ttacaggatg aaatagagaa
     1321 agtaagaagt gataaaagta ttgatggtga aacagccaaa ttaaaaatag aagctttaaa
     1381 aaaagaaatg gaagagattg aaaataaaat tcaagagtta aaaggaaata aagcaaaaaa
     1441 aacaaataaa gaagaaaata atgtttcaga agtaaataat aatctattag agagaaaaaa
     1501 agaaccttct aataatgaaa atgtaaaaca attatatgat gtttttgaaa gaagcaatgt
     1561 agacgaacaa tgggataata cttatcgtgt tctccaagat gataaaaact taaaagtaaa
     1621 atttaataga cctatatagt ttatttataa aaatagttaa tattttaatt atcaccactt
     1681 tttagaagaa tacacacttt attttaaaga cccacatatt tatttacagt tagcacattt
     1741 atatgtggtt ttataactat tatttacaag tctatattga taacttttac atagtatata
     1801 atgtttaaat acttattcaa atttatctat acataaacaa aaaaagagat tttaaaatct
     1861 ctttttagat tgttgactta tggctttttg atttaataaa atcaagaagc cgtttgaatt
     1921 ttatctgttg gctgataccc ttctgatatt gaaacaccat ttttgtttcc aaaatcgttt
     1981 aaaattaaga acttattatg aatgactatt tcattatgaa caataagcag ataacatgaa
     2041 tcttcaatca atgttttaac agtagcatta aatgcttgct tatacagttc tatcgtttgt
     2101 ttgaactgtc cattaaagta aatatgagtc aataacataa ctatatctac acaaattaat
     2161 ataaattttt tatactgtac attatctaac tgttgattat ctgtattaca acatgattga
     2221 atttaaaaag ccatcaacac atagaatgtt gatggcctga aaatagattt aaaatctctt
     2281 ttttattata tattaattat tttttaaatt tatatttttt gcatcagatt ttctaatagc
     2341 taagtcaaac cctctaaatc taatcactat agggtcatta agaggagcta tctttttcac
     2401 ttcaacttca gtgccatcta ttagtcctaa ggctagaagt ctcttcataa gtttttcatt
     2461 tccagctata ttgtctataa ttcccttttg tccaagtttt aagttgtaaa cagtcattat
     2521 taacacctcc taatgaaaat cattatcaaa ctttatattt ataatatata acaaaaagag
     2581 caaattgtca atatataaca aaaaataaac atactaagtt agtatgttta taaaaaatag
     2641 tatacatggg atactaatag catctagcaa aaaaccactt actaatggta caactaaaaa
     2701 ggcttttgga gaatcaccat atttttgtgt caaagaactc atatttgcca gagcgtttgg
     2761 agtagctcct attccatgtc caatcaaacc acttattatg atggcagaat caaaatcttt
     2821 tccaagtact ctaaagcaaa taaatattga aaataggatt ataaagatta cttgaaaagt
     2881 tactattaag agaataggag gtaataatgt agataattca aataaatcta tattcataag
     2941 tgataaagtt aaaaaaattc ccaatgaaag ttcttttaaa aaatctataa gtttaaaatt
     3001 taattctagt atatttatct tatcatttaa atttctaaaa ataacagccg aaaacataca
     3061 agttacaaca gagggtaata gtatattaaa cgttatatag aagcatctac ttataatttc
     3121 tcctgtattt atacaaataa atatgagaag aacctgttcc aaaaatacag atggtgttat
     3181 tgtttcggag aagttattat tttttatcct attcttattt ctaaaaaatt tacttccaaa
     3241 tctctttaga tttctattat atctaggaat cgacaagtct atatttgatg atggttttaa
     3301 tttatattta tttattagaa atttagctac aggacatccc aatataccac ccataataag
     3361 tcctattgtt gcagcagata atccaacccc aactgctcct tctataccga gattttctat
     3421 tgtaagtcca aatgcagcag aataaccatg accaccttcc atagatacat ttccacacat
     3481 aagccctaaa agaggttcaa gttttataat ctttgatatt gtaatagcta atatattttg
     3541 acaaaatgcc aatactccac acaaaatcca atatctaaat aatacactac cacctttctt
     3601 tataagattt atgcttattt caagtcctat agttgtaaaa aagaaggtta tgaaatttgg
     3661 cattaaattt ccacttatag atatatctgc tatattaaaa tatttaaaaa ataaatttag
     3721 aaaacaaaat aatagacctc ctattactgg tgcaggtata cagaaattat ttaaaaagtt
     3781 aactttattc ttcaataaat taccaattaa aaagaagaat atagatagtg tcaaggtttg
     3841 cactatatca aaacttaagg taatcaaaat aagtcctcct ttgtaattca aagttttaat
     3901 tatataactt aaattcttat gtattgaaat tttattatca aatgtatatt aataaatgta
     3961 tattaaaaag aatcagtaga tataattaga tagtcataaa ttaaattaaa atattttaat
     4021 gagatatgac ttttttagaa ttaaaaaccg tagtatattg atataataga aataagactt
     4081 aaaaatctgt tgatagtatt atttataagt acagtaggag gataaaatgt ttgaaaattt
     4141 acctaagata gatttacatt gccacttaga tggaagtgtt agagtagaaa ccatgcttga
     4201 tatagctatt aaagagaaaa tagatttacc ttcaaataat atggatgaaa taaaaaaatt
     4261 agcaaaggtt tcttttaatt gtacttcact agatgaatat ttagaaaaat ttgatttacc
     4321 cttaaaagta atgcaatcta aggaaaattt aaaaagaatt acttttgaac ttttggaaga
     4381 tgcaagtaga gaaaatgtaa aatatattga aattagattt gcaccattac ttcacaccca
     4441 aaaaggtatg agtgttaaaa atataattga gggtataatt gaaggaatta gagaagctga
     4501 aagtatttat gatataaaag gcaatttaat attaggttgt atgagaacaa tgacctcaaa
     4561 agaggcatta ttagtaattg aagaagggaa atcatttgta aataaaggtg ttgtagcagt
     4621 agacttgtgt ggtccagaga aagagggatt ttgtaaggaa tataaagatg tttttaaatt
     4681 agcaagagaa tatggatata aagttacaat acatgcagga gaagctgcaa gtggagaaaa
     4741 tgtattggat gctataaata ttttaaaggc ggataggatt ggtcatgggg taaaaattaa
     4801 agaccataaa aaggcgtata atttagttaa agataaaaaa atattgcttg aattatgtcc
     4861 aactagtaat gtacaaacta agacagtaga ttcttatgaa gcacatccat tttatacttt
     4921 ttataaagat aatttacatg taagtataaa tactgataat agaactgtat cagatataaa
     4981 tttaagttca gaactaaatg ttatatttga tacttttaaa ctaggattag aagactataa
     5041 aataatttat agaaatgcag tagaagctag ttttgcagat aaagaaacta aagagtattt
     5101 gaatagttta atttaataga taaaaatatt taaactttaa attaagctat taaattgata
     5161 aaaatacatt gtattaggag aaaaaatgtg aaagattatg aagatgttgt ttatattaaa
     5221 ctagtcaaaa aagaacttga agaatggaag aaaaatatat taaagaaacc ttctatattt
     5281 gacaaagcat caaagggtat acagaataaa tttaatggag tattgccaga taaatatcat
     5341 gaaataatta cttcttctat aaaagtaatg acaaaaaccg tactgtttgg atataaatat
     5401 gctacaaagc caccacttag aaatatgtca ttagaagaaa gagaaaaatt agttttagaa
     5461 aaagctaaaa cttacaagac tacagcaatg attgaagggg caggaacagg ggcaggagga
     5521 atcctcttag gtttatctga ttttcctttg cttttaggta ttaaagtgaa atttttatat
     5581 gatgtagctt caatatatgg atatgatgtt aaagattata gagaaaggat ttatatatta
     5641 aatatattac agttggcttt ttctagtcaa cagcatataa acaaaacttt taaattgatg
     5701 aataactggg atgaatatgt aaatagtcta ccagaagaca tcaatgattt tgattggaga
     5761 acatttcaac aagagtatag ggattattta gatttagcta agtttttaca actaatgcct
     5821 ggaataggag catttgtagg attttatgtg aatggaaaat taattgataa attatgtgaa
     5881 actgctgtgt ctgcttatag attgaggatt cgagagctta agttataaga aaaattataa
     5941 cataaaaaat tagttataca atatatctat atgtatctaa ttttgcattg ataaaaacaa
     6001 ttatggataa aaggtaaagc atgatagaat ctaatgtaga ggtttgtatt ttaatggaaa
     6061 aatttagttt ttattgctta agatacaaat tgaattacga agagattgaa ttatgaagag
     6121 aagtaattgg gaggaatatt ttcatgacaa atttgccaaa acaatttgat agcttcaatg
     6181 aagctagaca aaaaggattt ataaatgcaa aggaacttaa ggaaagtggg aaaaagatgg
     6241 ttggagtttt ctgtacattt actccagttg aaattccaat ggcagctgga gcaactgtag
     6301 taggtgtatg cggcgttagt gaagagccaa tacctgatgc agaaagggtg cttccaagaa
     6361 atctttgtcc tttaattaaa tcaagctatg gacatgcaat aacagatacc tgtccttatt
     6421 tctatttttc tgatttgttg ataggagaaa ccacttgtga tggtaagaaa aaaatgtatg
     6481 aagaacttgc aaaagttaag ccaacatatg taatgcattt gccaaatact tcaaaaggag
     6541 aatttgccta taaactttgg aaagatgaaa tgataagatt aaaagaagag gttgagaaat
     6601 ctttaggtgt aactataact gaagaagata taagaactgc tataaaagat aaaaatgaag
     6661 aaagagaact tttaaaagag ttttatgcac ttggtaaatt acaaccatct gcacttacag
     6721 gccttgagtt acataatgtt ttatatcaag caggttttaa atttgaccgt gcagaactta
     6781 aacattcttt aagaaaagtt atagatgata tgaaagaaag atatgaaaaa ggtgaatgtc
     6841 ctgttcaaaa agataagcca agaatattga taacaggttc tcctattggt ggaataagcg
     6901 aaaaaatagt taaaacttta gaagatgcag gggcatcagt cgttgcatat gaactttgtg
     6961 gagctattag atcaaacgat ttattggttg atgaagaaat agaagatgta tatgatgctt
     7021 taacacaaaa atatataaat ataggttgtt cttgtatgat gaataatgac aatcgtattg
     7081 aattattaga tagaataata gatgaatata atgtagatgc tgtaatagat gtagttttac
     7141 aagcatgtca tacatttaat atagaaagtt atagaataag agaatttgtt acaaaagaaa
     7201 aaaataagcc atttatgtct cttgaaactg attattcaaa atcagatact gaacaattaa
     7261 gaactagatt tgaagcgttt gtagagatgt tgtaggaata aataaaagta ggaggaacta
     7321 tgtttagtat aggagttgac tcagggtcag tggcaacaaa gggtgtgcta tttgatggtg
     7381 aaaaaattat taagaaaata attataccaa ctggatggag ccctaaaagt acatctaaac
     7441 aggtctatga gttgctaagt agtgaaattg acaaaaaaga tataaaaaag gtggtaggta
     7501 caggttatgg aaggggagta atggattttg ctgataagaa agtaacagaa ataacttgtc
     7561 atactagagg tatttacttt ttaaacaaga acataaggac tattttagat gtaggtggtc
     7621 aagatagtaa agttataaat ttagatagag atggaaatgt atttaacttt ataatgaatg
     7681 ataagtgtgc tgcaggaact ggtcgatttt tagagattac ttctaacctt ttaggaagcg
     7741 atattgaaag tatagataca ttagctaaag gatatgaacc agttaatata tctagtatgt
     7801 gtacagtttt tgctgagtct gaaattgtaa gtcttctagc tcaaaacatt tctactggag
     7861 aggtagcagc agggatactt aaatctattg caaataaatc cacctcaatg ttagcaagag
     7921 gtgaggtaat agatgaagtt gcatttactg gagggcttgc aaaaagtaaa gaactagtaa
     7981 aaatgataga agaaatactg ggtaaaaaaa tttttattgc agaagatact caaataatag
     8041 gagctttagg agctgctgta ataggattta gataaaaaca tataaaaaga aaatgagaag
     8101 ctgaatttat tataaataaa ttcagttttt tattttattt tatttattta taaaggaaaa
     8161 ctttggtata attgatttaa atgattgtat atttgtaaat acttgatatt tagtcatata
     8221 taacattata attttaaaaa ttagctcaaa ataaggggga gaaagcgtga aaaaatttac
     8281 ttcaaaaaaa gtaacaaggt ttgttgcgct gtttttaata tctattttag taatgtcaac
     8341 aattcctgta tcagctgaca atagcaatac attgaatcag ataaaaaaag cagaatacag
     8401 tgagacgtat aaacaatatc taaatgatgc taaaaatgga tgtacagaaa aatataatgg
     8461 gattatacca aatccatatc agttggaagg tactcaaatc caaagtaaag gaagaacagc
     8521 tccaacatct tatgacccta gaaaattagg tcttatgaca tcaataaaaa atcaagagga
     8581 tttaggaatc tgttgggatt ttgctgcaat ggcaacctta gaaagtttct taaagttaaa
     8641 taattatgga gactatgatt tatcagaaga acacttaaga tggtgggctt cagatggtga
     8701 atatggatgg tctgtaaatg atatgaatgg tgctttaaat tatgaagcta tgggatattt
     8761 aacatcttgg tcaggtccta aattagaaaa agatatccca tacaatggta gagtatctaa
     8821 agcacaagga gcaaaaaaac caactaatat gaatacagca cctactgttt ttaatgtgac
     8881 agatgctgtg tgtgtaagca atgacataaa ttctacaaaa aatgctatat tacagtatgg
     8941 agctgtaaca tctggttatt atgaagatat taaatatcaa agtgatgacc aaaattcata
     9001 ttattgtcca acaaaaagtt ttaatacaaa tcatgctatt tcaattgtag gttgggatga
     9061 taactattct aaagataatt ttaatgcaaa tattagacca tcaaaaaatg gtgcttggtt
     9121 aataaaaaat agctggggag attataattc tgagggtgga tatttctgga tatcttatga
     9181 agacaagaca cttatgtctg atatagataa tttttcaatc aaaggtggta aaaaaccaaa
     9241 tgatgatgag aagatgtatc aacatgacta tgcaagtatt gtacctttaa tttcaaaaaa
     9301 gataacagct gcaaatgtat ttgatttcaa tagaggcgat gaaactctaa aatctgtaat
     9361 gttcttaact gaaagtatag gagctaagta tgaagtatat tatgctccag tagtaaatgg
     9421 tatacctcaa gaaaacaata tgaaaaaatt aaaagaaggt actgcccaat attcaggata
     9481 cataacagtt ccaattgatt cttttgatat accagaagga aagggtgcaa ttgtagtcag
     9541 tatagagggg aaaaatggag agtcaactat aggttctgag tcaaatgtac caggatatga
     9601 tacatttaaa gcaaaagcta atttaggtga aagttatatc atagataata ctggaaaatt
     9661 ctttgatata aatagagata gtaacttcta tccgtgtaat tttactataa aagctgttac
     9721 agaaaagtct tctggtgaga gtatcccaaa tgagtcttta attggtagtg atagatatga
     9781 aactgctatc aaagttagtc aaaatggttt caattcttca gaaaatgtag ttttagtaaa
     9841 tggcagttca atagtagatg cattggcagc aactcctttt acatctgcta taaattcacc
     9901 aatattattg acacaaaaag aagctttaaa ttcaaaaact aaagctgaga tacagagatt
     9961 aggtgctaag aaagtatacc taataggtgg agaaaattct ataagtaaag aaattgaaca
    10021 acaattaaaa agcttaaata tatcgataga gagaatatca ggaagtgata gatacaagac
    10081 aagtttatta ttagctcaaa agttaaatgg tataaaaaat gtatctcaaa tagcagttgt
    10141 aaatggagtg aaaggattag ctgatgcaat tagtgtaggc gctgcagcag cagaaaataa
    10201 cataccaatc atacttgcaa atgaaaagag cgaattgcaa ggagctgatg aatttctaaa
    10261 ttcattaaat atagagaaat cttatattat tggaggaaca gcatccctat caaacaatct
    10321 agaaagtaag ttaaaaaatc caactagatt atctggtagt agtagagacg aaactaattc
    10381 aaagataata gataattttt ataaaaaaga tactttaaaa aatgcatttg tagttaaaaa
    10441 tggaatcaag aatcaaaatg atttaataga tggattggca gttggtcctt taggagcaaa
    10501 aactgggtca ccagtaattt tagttggtga taaattagct gatagccaaa aagaagttct
    10561 taaaaataag actttagaaa aagtaactca agttggtgga ggagcaaata aaaatgcatt
    10621 taaagagtta gtaaaattaa aaagtgctaa atagagtact taagtattaa catatattag
    10681 tgtgtattag taacttatct taataaaagc tgaaaataat tacttcaaat tattgataaa
    10741 taagataatc aatgattgta aagttttgtt ttcagcttta aatttatgta gtataagttt
    10801 aaaaacataa aaaagggtta ctttatatag aaatatagaa aatcttacat tttatatata
    10861 aataacccat atttaattaa ctttttatag tgttctaata gatattattg aacttaatgg
    10921 taaatataat gtgtaagttt catttaaagt tggattagat tcatctatat atcttgagca
    10981 aagtgtaatt gataagctat ctgcttctat aatttcacca tatagaggac aactgctaaa
    11041 gtaagaatgt acttcaacat atttatttaa gttttcttga acataatcgt aaaaacggtt
    11101 ttttgtagta gtagccataa aatcaactcc tttatttttt attaaagttg aaacttttaa
    11161 aaagataaat atgattactt ctattagaaa ctaagttaaa aaataagcaa atagatatga
    11221 aaattagaat aatatattat ttatatctta attataacac tagataataa aaaatgctat
    11281 ataaaattca gaattttcga aagtttttaa gcttattttt aaatatacta ataatactat
    11341 tgacattaga tagaaaaagt attatattga ctatataaat tattttagtc aaactggagg
    11401 gaaaagatgc ctaaatcata ttctgataaa gaaagagagt atataataaa aagacttaaa
    11461 gaagaagcaa gattgtgtat ggagcaatat ggtattagaa aaactactgt tgatgagctt
    11521 gtaaaacggg taaaaatacc aaaaggaact ttttatctat tttttcaatc aaaagaatta
    11581 ctcttttttg aggtattaag agatatacat gactctatac aaaaagaaat cttatatgag
    11641 ataaatagag tagatgaaag tattacatgt gaacaattga cagatatatt tatgaaattc
    11701 tataggatgg ttgatagtac ttcaatttta aatctcatga taaatggaga atttgagata
    11761 ttggtgagaa agttaccaga ttctataatt gaagaatact ttagacatga tgactttgaa
    11821 attggagaga ttatatcttg cataccaaat gcaaaaaata aagacataga aagctttagt
    11881 ggagcattta gagcagtttt tcttaccatg ctatataagc gcgaggttgg aagtaattgt
    11941 tttgaagatg ctttaagatt aatgataaac ggattagtta tacaacttat ggaatagaat
    12001 ttaaattttg tttgctaata acttttatta aaaattatta acaaacaaaa aaattatatt
    12061 ttattgacca tatagtcaaa atagtcaaaa atttgaaagg gtgatattga atgattaaag
    12121 ttgatgattt atcatttagt tatacagata gagatttttt acaaaatatt aactttgaag
    12181 taggaaaagg ggaaatacta ggatttttag gaccatcagg tgcaggaaaa tctacattac
    12241 aaaagattct aattggaatg ataactaatt atggaggaag tgtcattgta aatggagtag
    12301 agagtaaaag acactcaaat aaattttatg aaaatatagg agttgatttt gaatttccta
    12361 gtttatatga aaagttgact gcaatagaaa atttaaaata ttttgggtct ctatattcta
    12421 aaaaactact atctattgat gagttattaa aatcagtagg actagaaaat gaatcaaata
    12481 aaagggtatc tgagtattca aaaggtatga aatctcgatt aaattttata aaagctttat
    12541 tacataatcc agatatttta tttttagatg aaccaacaag tggtcttgac ccatcaaata
    12601 gcaaggttat gaaagatata attttatcag aaaaatcgaa aggtaagact attattttaa
    12661 caactcataa catgttagat gcaacagagc tttgtgacag agtagcgttc attgtaaatg
    12721 gtaaaatatc tgctttagat acaccacata atcttattat gtcaaaagga gctattaaag
    12781 ttagatatac ttactttgat aatggagaaa aaacttctga atgttttctg aacaatactg
    12841 ctaatgataa aaatttgaat atgcttattg agaaaaataa acttttatca atacatagta
    12901 gtgaaccaac attaaatgat atttttattg agataacagg gaggaattta caatgaggct
    12961 aaaaaatctt attttaggag atataaagtt tcaatttaag tatggttttt attttttata
    13021 tcttttttta agtattatat atatttgtat tatcaatgta tttccaactt ttatgagaga
    13081 aaaaatagct ataattatga tttattctga cccagcagca atgggattgt tttttatggg
    13141 agctattgta cttcttgaga aaagtcaaag ggttttaaat tccttagctg tatctccagt
    13201 aaaagtatct gagtatattt tatcaaaggt aatttcatta ggagtaatat ctagtattgt
    13261 agctatgttt attgctatta cattaaattt agataatatt ataatatcaa ctataggtac
    13321 ctttttttct tctattatat tttctttgct tggattgata attgcaagta aagcatctag
    13381 tttgaatcag tttattgttt taagtattcc tattgagatt atatgcttta ttccaccaat
    13441 actgaatgta cttcttgaca caaaatcata tgcaaatttg taccctttta atatatgtat
    13501 ttctcttatt agtggagata aaaattttat aatgattaat attttaattt taatatcaat
    13561 tataataata atttatttta taacttatta ttttatttgt agcagttgga aaaaggtagg
    13621 tggagtaaaa ctatgataaa gattataaat gcgtttaaac aagagtttga acaaattaag
    13681 agagatgcta tgctttttat agtatgtgtt tctccaatat tatgtggagt atttataaaa
    13741 tttggaatac cattaataca gaatatatca cttaacaagt tttattatca attaaattta
    13801 gagccgtatt tcttgatgtt tgatttactt ttagctttta ttacaccttt tatgttcttt
    13861 tttgcttcta caatggtcat attgggagaa attgatgaca gcatttcaag atatttaata
    13921 gtaactccgc ttggtaaaac tggttatctt atttcacgat ttggaatacc aggtatctta
    13981 gcatttatta ttactatgat tctacttatc tttttttctc taactaaaat ttctctcttg
    14041 ttaaacttat caatttcttt attagtttta ttacaaggag ttattatatc aatattagtg
    14101 atttctttat catcaaataa actagaagga atggtaatta cgaaattctc aggtgttttt
    14161 atgatgggta ttttagcacc attttttatt ttaaataaag cacaatatat tctgttcttc
    14221 ttaccaactt tttggatatc gaaggcattt aaggaagata attatgtcta tatgtttatt
    14281 tcaattgtta tatcattaat atggatttta ttattattta aaaagtttag taaaaaaata
    14341 gttaaataag aaaatttaat atataaatgt aaggttgact tgacagtaat gttgaaaaat
    14401 gttacattta agatgtaaag ttaaccttac atgaatggag caaatatgaa aaataaaata
    14461 aagatattaa gagaaaaatt aggattgaca caagaacaac tagggagatt agttggaaca
    14521 tctagacagg ctataaatgc tatagaaact ggtaaaaatg aaccttcaat atggttggca
    14581 tatgatattt caagaatttt taatgaacca attgaaagtg tatttttatt tgaagaaagt
    14641 gaaagaaaat caagagcaca gataagtagg ggagaatatt atggctataa gaaagataag
    14701 aacatttgat gatgaaattt taagaaaaaa gagtaagtat gtagaaaacg tagataataa
    14761 gataagagaa atattaaatg acatggcaga gaccatgtac aatactccaa atggaggagg
    14821 attagctgca tgccaagtag gagtattaaa gcgattagtt gttatagatt taggagaagg
    14881 acttattaag cttgttaatc ctgaaattat tgaacaagaa ggggaacaaa ttgttgttga
    14941 aggatgttta agttttcctg aagtatgggg taaattaaaa agaccaaaaa aagttacagt
    15001 gcaagctcta aacgaatacg gtgagaaaat agaaattaaa ggttcaggtt ttatggcaaa
    15061 atgtctttgt catgagatag accatttaaa tggtatagtt tttacagata aaattattga
    15121 acatgtaaaa ttgtaagata gattggttat tgaatttata aaatatttta tagtaaggag
    15181 ttaaattatg agattatggc ataaggattt aattgatgta ttacctaaaa atcaattagt
    15241 aagtcaatgg agagagctac ttgctataaa aggttctata gataaaaaag gtactccaaa
    15301 ccatctatta gtgaataaag tattgaatta tagtatagat gaatttaaat tttatacaaa
    15361 aattgtacat gatgaaatgt taaaaagaaa ttataaacct aatgaactta aatatacaag
    15421 tatattaaaa tggaaaaata gaaattttgc aaatgatata tctaatgaac atagtttaaa
    15481 tttagaaaat ttatatgatg attggcataa taaaatgtat ttaaagcaat gcttatataa
    15541 tttagaagaa aaagcaacat gtggaggaat accaataaat gaatggaata tattactttg
    15601 taaatatagt aaagattatg aattgtggtc tggaaatatc atgttttaat tactatagat
    15661 taacattata gtattattgt atataaagag gtgattattt gtataataaa ataaaatata
    15721 ttccaataaa ttgtgaaata gcttgtaata aattaaaaag gagtatgcca tataaatggg
    15781 atttaaatat ttatcgtgga tgtgagcatg gatgtaaata ttgttatgct atttactctc
    15841 ataagtacat caactccaat aattattttg aagagattta tgtaaaaaca aatatagtag
    15901 aaaaacttga gagacaactt aaaagcagta aatggaagaa agaagtaatc aatataggag
    15961 gagttacaga tagttatcaa ccaatagaag ctgactataa aattatgcca gaaatattaa
    16021 acctgctaat aaaatacaag acaccagcta ttatttctac aaagtcagat ttaattttaa
    16081 gagattacga tttaattgat aagctatcta ggattacata tatcaatgta gcatctacaa
    16141 ttaccacagt aaatgaagat acacaaaggc ttattgagcc aaatggtgtg gattcaatga
    16201 gacgatttaa aatgttaaaa gaatttagaa aaactaacgc atcagttggt ctacatatta
    16261 tgccaattat accatatatt acagatgatt ttgataatat aaattcgcta tttagacatg
    16321 caaaagaatg taatgtacat tatgttttac ctgggacttt atacttaagg ggtataacta
    16381 gaggtgtttt ttttgacttt gttaaaaaag agtttcctga attatttgat aaattatcaa
    16441 tactatatag tgcaggttca cttaataggg agtataaaaa tcaattgtat aaaatggtta
    16501 atgaacttag atataagtat tctctatcaa gcagttactc taaaataatg aaggaaaagt
    16561 taaaaaactc aggagatgta cagatatctt tttttgatta gttttatata aaaaaatttt
    16621 ttttactatg atgattaaaa tttattaaaa ttaaaattta ttgacaaata taaaatttaa
    16681 atatataatt aattcataaa ataaaaatat gaaaggatga tgttgttcgg tgatatttga
    16741 tatcataaaa tcgttgatat aagtattgct ggaaaagagt gaataaaatc aaatttaata
    16801 agcaaacaaa gtaaagctat agttttattg ttgtgcttaa aaatatttgg ttctatttta
    16861 ctcttattta aactcatctt tttgaagtta aaccacagag taaactctgt ggttttttta
    16921 ttttaaaaat aaattttaag gagtggtatg ttttgttaaa caaattatgt atgtatttag
    16981 aaagaccaga gctttataaa caaagtgaaa ttaatttttg ggatgatgag tatatttcaa
    17041 agcaattatt aaaagcacac ttagacacta attttgaagg agcaagtaga aattttaact
    17101 ttatcgagga ttctgtaaat tggatagtta cagttgcaaa tccagctaat tatcctaaat
    17161 tacttgattt aggttgtggt ccaggtttat atgcagagaa atttgcacaa aaaggttata
    17221 aggtaactgg aattgatttc tcaaaacgtt ctattaacta tgcacaaaat agaaataagg
    17281 agactaattt aaacataaat tacttgttcc aaagttattt aaatatgaac tataatgagg
    17341 aatttgattt ggctacttta atttattgtg attatggagc tttgtcaact gaaaatagaa
    17401 ggttattaat ggaaaaaatt tatgatagtt taaaacctgg aggaaaatta atattagatg
    17461 tatttacaat aaataaatac aataattttg aggaaattaa atattgggaa ataaatgaag
    17521 atggaggatt ttggagtaat gaaaaatata tgtgtctaca agataattgt aaatacaatg
    17581 attataatac attagaacaa actcttgtaa taaccgaaaa agatgaaaat gtattttatg
    17641 tttggaatca ctatttttca aaagaaagtt tcttattaga agttgaaaat attggattta
    17701 agtctgttga gttttttagt aatgttaagg gtgaagcata ttctgacgat agtatgacta
    17761 tgggattagt tttacaaaaa taataatttt tttaaaatga ataaataaaa acttgaaacc
    17821 tttaacaata aaataacata ataaaagctt acatattgaa aaatggaaac ttttatgtta
    17881 tttttagtac attacaaatt tgcttatata aaaatcaata cttgaattat tatttttatg
    17941 tgaaatttat ctatagtata tttttataaa caagtaatgt tattaaaatt aagtatgtag
    18001 ttaaaagtgt atagcaaaaa ggctgttata ttaatgttag aggttcaggg aaaatatata
    18061 aagcaaagta tttacagata atgtagattg cacagctata agttagataa tagaattatg
    18121 taatcaagaa tttacaacaa agtataagat aaggataatg ctaaattgcc atacaggtgc
    18181 tagatgtgta aatggattta attaatacta ttgataaagt gcaacaagca cataaaccaa
    18241 ttgatgagat tattaaaact ataagagata tagttgacat aattaaaata ttggaacttg
    18301 tttataattt taaatcaaat caataatgat taagattaaa ataatattaa ggcatcaatg
    18361 ttataagagt acaatataag gagtactata tagtactcct tacttataca tattaggcaa
    18421 taggaaattt tttcatagtt aaattttcaa gtatttttta ggaaacttta taattgagaa
    18481 ttaagggtat agtatgtgat gaacttactg aatttataat taggttatat aatggaataa
    18541 tcatcaatag ttttacttta gggaaaatat atgcttaagg aagtatagct tactaatatt
    18601 attaaaatgt atatttaaaa tgataaaaaa agaattagac aagtatttag taaattgact
    18661 atgcaaaatt ctatcaagca ggaaccctat gactttagtc atgggaggtt cagtaggtaa
    18721 cttgttgaat taagtttata ttatttaaat aaactactta atttagataa tataaaaagt
    18781 cagaatttta agaatagagc tatataatct tagctattag gagaattact atgataggaa
    18841 tatattttag tggaacagga aattcaagat actgtgtaga aaaattttta caagaatatg
    18901 atataacagc aaattcttat tctattgaag ataatgaact tttacagcac atcaataatc
    18961 atgagaatat tatttttagt tatccggtgc aatatagtaa tgtgccaaaa atattgaaag
    19021 attttataat aaatcattca actttgtgga atggtaaacg tgtttttatt attgcaacta
    19081 tggctctatt tagtggagat ggctctggtg tgttatctag aatattaaaa aaatacggtg
    19141 ctataactgt tggtggatta catgtgaaga tgccagatag tataggagat gaaaaggtat
    19201 taaaacattc tttagagtat aatagaaagc ttgtaataaa agcagaaaag aaagtaacta
    19261 aagctgctaa aaaactgaaa catgggtcac caccaaaaga agggttaggt tttttgagtc
    19321 atattgtagg gttatttgga caacgattgt attttatcaa caaaacaaat atgtatacag
    19381 ataaactaaa aattaatact gaggaatgta ttgtatgtgg taaatgtgtt aatttatgtc
    19441 caatgaagaa tctagtaatt aaaaattgta tgattgttgc aaatgggcaa tgtacaatgt
    19501 gttatcgttg tgtaaataat tgtgctaaaa aagcaattac attgttaggt aaaaaagttg
    19561 ttgaacagag taatattaaa aaatatatgt aagaaattta aagttttatt gaatgtaaat
    19621 tgatgtatat tttatgttag aaagagaggt taaataatgg aatactacat agttgattca
    19681 tttgcaacta aactatttaa aggaaatcct gctggagttt gtgttcttga cagaagaata
    19741 cctttagagt taatgcagaa aatagcagaa gaaaacaatt taccagaaac agcatttgta
    19801 gttaaaggta aaggaaatta tgaattacga tggtttactc ctaaagcaga aattgattta
    19861 tgtggtcatg caactttagc agcagcttat gtaatatcaa attttattga tgttaatgta
    19921 aaaaggatag atttctttac tcaaagtgga aagcttgaag ttacacgaaa tggaaatcta
    19981 tatgaaatga tatttccaga aataatgcct attgaaatag agttatcacc acaacaagct
    20041 aatttgatag gctgtgtacc aagtgctgtt tattcttcaa gagatttaat cttattgcta
    20101 aacagtgaac aagaagttat aaattataaa cctaattacg cacaattgcg taaattgaca
    20161 gactggttag gtataataat cacagcacaa ggaagtaata cagattttgt atcaagatat
    20221 ttttgtccag aattagattc agaagaccct gttactggtt catcacattg taatcttatt
    20281 ccatactggt ctgaaaagtt gggaaaacac aaaatggtag ctgctcaact ttctaatcgt
    20341 ggtggtataa ttcagtgtga agtgttgaag gataatactg tgaaaatttc tggtgaagca
    20401 gtcttgttta tgcagggaac aataaagatt gatatttaaa taagtataat aactaattta
    20461 ttatgaaaaa taattaattt tcttagtgtt aacaataata aagtagagtt aaaaaacttg
    20521 atttcactta aatcaatatg atagatgttg atactaagat tgacaagcat gattaaaaaa
    20581 gataatattt aatctataaa gataaaaaca cagtctggtt ggtagtccag acgcagcaat
    20641 agctgtcagt aacctgcctc cttggttgtc cgttctttag aaagtaattt aaggaggaac
    20701 tagcaatgga caaaattagt ggaaaattaa cagtcttatt tgaagaacca ttttgggtag
    20761 gtatatttga aagacaagat ggtaaaaaat atgaagcatg tagggtagtt tttggagcag
    20821 aacctaaaga agtagaagtg tatgaattta tacttgaaag atttttctca ttagattttg
    20881 gtagcattaa attagaaaaa aatgtgacca aagacaacat tggatacaaa agaatgcaga
    20941 gaaaagtaaa aaaggaacaa gaaaaagaaa ctatagggac taaggctcaa aatgctctta
    21001 agcttcaata tgaagaaaga aaacaggata gaaaaaacct agctaaaaat agaaaagaag
    21061 aagaaaaaga aagactattt aatttgaaac aagaaaagag aaaagctaaa cataagggtc
    21121 attaatttta attaaatatc tttttaataa ttgctagaaa aaggtgtata aaatttttaa
    21181 ttttatacac cttaatttat tgaaaagaat aaggttttat atttcaatta ctttaaaata
    21241 taaaaatata ctaataaaaa ttgtaaaaat agtataaatg gtataccaaa tgtaaattta
    21301 atgtgtttag ttttatgtct aaaagaatac attccaatta tagaacctat actacctcca
    21361 attactgcta tagacataag agtagcttct tttattctcc attcgtttct aattgctctt
    21421 ttcttatcta tgtacattga aaagaaagca atagaattaa tcactataaa atatattaga
    21481 ataaaatttt tcacagtctt gcctccataa aaatatatta tacattataa aacaagatat
    21541 ttgttttgga aagttaaatt tgtaattagt acatataaaa tatttgataa gaacaaatat
    21601 aatattaatt ggaaaattta aaaaaattaa acaattagca aatagggggt atatttataa
    21661 gacacaaagt gataaaatct atttagaaaa taatgtaaaa taacaggagg ataaagcaaa
    21721 atgaaattta tagtagaaaa agaagttttt gataaattag aaaatgtatg ctttggtgta
    21781 gtagttgcaa aaggaataga taatactaaa gaaatagaaa gaataagcaa ccttttagat
    21841 ataagtatag atagagtaga agattacttc aaagataaaa aagtaaagga atcagaagaa
    21901 attataccat atagagaggc atttagaagt ctaggcatga acccaaataa atttatgagt
    21961 tcgatagaag caatgacaac aagggttgct aagaataaga aacttcctca tataaatcct
    22021 attgtagatt taggaaactc tatttcttta aaatatttac ttccaatggg tgctcatgat
    22081 atggatttta gaaatgatga tgtatatgtt agattttcta aaaaagggga taaatttgta
    22141 ccatttggag aaactgatgt tgaattaatg gaagaaggag aattaattta ttcagttgga
    22201 gacatggtaa aaactagaag atggatatgg agacaaggag aagaaggaaa aattactaat
    22261 agctcaaaaa atatattttt tcctatagat ggttttactg atgctaattt agataaggta
    22321 atgagtgcaa gagaagaatt agcaaaattg ttaaaggaaa tatttaattg tgaaattaaa
    22381 gtaggttttg tagataaaga taatcctgaa atggaaatat aataagttgt tgaatacttg
    22441 ttaagatatt atatttactt aatgatgtta aaaaggtatt aaaaaagaaa ggtcgtagta
    22501 aatttgaaaa aaactataat aaagaatgta aatccaatga ttgaaaataa gtttatagga
    22561 ctttttgaaa tagagtataa aaacaaatta gatgaagata aggtttggat ggtagcatca
    22621 agaaaaagta gtgagcaatt aaaaaatatt tatttgaaga atgaagaaga tagtgtagat
    22681 gcagttgtaa tagttgggct tcataaaagt agtaaaaaat taattttaat aagacaattt
    22741 agagtaccta ttaatggata tatatacgag ttaccagcag gtcttgttga tgaaggagag
    22801 tctatagata tttccgttga aagagaactt agagaagaaa caggacttac tcttttagaa
    22861 attaaaaaaa ataaaagtat tgataaagtt tatttatctc ctggaatgag tgatgaatct
    22921 gttgcctttg tttattgtat atgtgatggt gatataacag atgaattttt agaaccagat
    22981 gaagagattg aagcgctttt aatttcgcaa gaagatgcaa aagagatttt acaaagtaat
    23041 cataagatag acacaaaagc atttttgatt ttacaaatgt ttgtgagttt aggagctaaa
    23101 ttatttgaat agatatatag aaaagaggtt atatagaaga gtaaaataat aaagtttatt
    23161 ttacttgtgt aaattataat ctcttttttt atacaataaa gtataatttt gtgtattttt
    23221 aaataagtga tttgactctt aaattttcat aatatgttat aaaccaattt aagatactac
    23281 aataattaaa attccaaaat tatttaacat atttgcaatt aaaatgagaa aatgtaaagg
    23341 ctgaatgatt gaaaaatttg atgtaaaata gtagcttagt ttaatactat agtttgtagg
    23401 gttaaaaatt agaaattgta agggagataa attctatgaa aaaattagtt attattttaa
    23461 ttagtatcat tagtatggtt agcttgacag cttgtaaaaa aagcaatgaa agtaaatata
    23521 ataatttaaa caacaaagaa tccggacaaa caaccattga aatggactta gataaaaatt
    23581 atgatacttc agacccattt gttaatgcac gtttgttttg tgtgtctaat gatatagatg
    23641 ttttagacac agagatttct tttaaaatgg atggagatag tggtattgtg gaaataaagg
    23701 acaataaaac agatgaaact ttatggagta atacatggca tggaagcgtt gataatgata
    23761 cattcacaat ttctctttct aatatacaaa aagagaaaga gtatgctata tgctttactg
    23821 gaattaaaat aaatcatgca gttgtaaagg ttacttttga aagcaattta gttaaggaga
    23881 aagaaaaacc ctcaaaataa attccagctt ttctataact tgatattcag tacataaaag
    23941 cagttgatat ttatgattga ctgctttttc ttatcaaaaa ggataggttg tgcattttta
    24001 cttgaaatag tttatgcaca tcaaaagttg gtgagcatga tattggaaat ggtaagcatg
    24061 aaatcttgat aatacaagga aataaattta agttttcaat taagtatatt tccataattt
    24121 agttaacaat aagctaatta ttatttatat tttgatatat aaataataat tgtag
//
LOCUS       DAGSRM010000050        23953 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.050, whole genome shotgun sequence.
ACCESSION   DAGSRM010000050 DAGSRM010000000
VERSION     DAGSRM010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23953
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.050"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            <1..597
                     /locus_tag="MJE33_002215"
     CDS             <1..597
                     /locus_tag="MJE33_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBY9648147.1"
                     /translation="GLIKESSGQVLLNKHNLSNLAEHRRTQIVSRVFQDPSLGTCPSM
                     TVRENLSLALNKGKLLNLRKCLRYKRDFLENLLEGVSLDLKKYLDVQVKFLSGGQRQS
                     LSLIMSCLTNPSVLLLDEHTAALDPKTSNEVIELTNKIVREKNITTLMVTHNLKHALH
                     YGDRLIMLHKGEVVLDVKGKEKEQLTVEEILEKFEYAV"
     gene            728..1750
                     /locus_tag="MJE33_002216"
     CDS             728..1750
                     /locus_tag="MJE33_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBY9648148.1"
                     /translation="MINKKRLASLILAGALSISMLTGCSQGGDSGNSKQESNSKGKEV
                     KKIGITQLVEHPALDATRTGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILITAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNENISY
                     VKTKQE"
     gene            complement(1914..2471)
                     /locus_tag="MJE33_002217"
     CDS             complement(1914..2471)
                     /locus_tag="MJE33_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBY9648149.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEK
                     REDILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPIKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
     gene            complement(2477..3553)
                     /locus_tag="MJE33_002218"
     CDS             complement(2477..3553)
                     /locus_tag="MJE33_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HBY9648150.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIVENPFTYTIYVRKTDLSNECVPIQIELEKSKMRFKK
                     GDRIYLDFPQEYLFCFRYNYNKKE"
     gene            complement(3554..4237)
                     /gene="modB"
                     /locus_tag="MJE33_002219"
     CDS             complement(3554..4237)
                     /gene="modB"
                     /locus_tag="MJE33_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HBY9648151.1"
                     /translation="MGTDWSPLWISLKTSILSTIITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWSGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            complement(4386..5198)
                     /gene="modA"
                     /locus_tag="MJE33_002220"
     CDS             complement(4386..5198)
                     /gene="modA"
                     /locus_tag="MJE33_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBY9648152.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEDKKDDGKQEKTTKSSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSADSSVSGMKDLTTDKVKKIAVGEAESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDAAKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            5391..6320
                     /locus_tag="MJE33_002221"
     CDS             5391..6320
                     /locus_tag="MJE33_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY9648153.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWEDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGLGGYDLTDTGKTIAKV"
     gene            complement(6481..6930)
                     /locus_tag="MJE33_002222"
     CDS             complement(6481..6930)
                     /locus_tag="MJE33_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648154.1"
                     /translation="MKNKNNKDKNMLNIKVLRVLGVLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGTDCIVFLLFIVECVYLVGRYILKVKINWSEIIENEKGNFINNLLVEKI
                     QEESNIVYNLLNSYVLPNIYKKARGLMFKTYHMLIYIKEKYYYVNIY"
     gene            complement(7133..8113)
                     /locus_tag="MJE33_002223"
     CDS             complement(7133..8113)
                     /locus_tag="MJE33_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBY9648155.1"
                     /translation="MKNSFKNCISLRNKFIIISIVFLLILLIVSYIFGKLVYNSSVGL
                     EQLIKNEDIVKIFSERKDKPLEKLKNYKTSELMIQSSNNYKLESLFITSNIKTRDTMI
                     LVHGIGSSYYEMLKVAYRYLDKGYNVLVYNQRNTGNSGGDNYTFGLYERYDLDSLVKF
                     VKNKFPEGRLGVHGFSMGAGTAAMHSEINSKDDKVDFYILDSPYSEMKDAIRMGVLEK
                     RIPDILINYVVTCGDLYNKFKSGFWYSDVKPYESVEKSNVPILFIHGTKDTVCNYQNS
                     KKMYDLVKHDKKDLWLIEGIGHVDGFEHDSTVYFNKIFKFIDSNVLSDKS"
     gene            complement(8318..9178)
                     /locus_tag="MJE33_002224"
     CDS             complement(8318..9178)
                     /locus_tag="MJE33_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HBY9648156.1"
                     /translation="MAMKWRDYADDVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKE
                     VPSKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELIDVNDI
                     EEVEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCD
                     KIIKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLN
                     TIKEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEE
                     EEEKYRRIFK"
     gene            complement(9495..10823)
                     /locus_tag="MJE33_002225"
     CDS             complement(9495..10823)
                     /locus_tag="MJE33_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBY9648157.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPKLN"
     gene            complement(10971..11300)
                     /locus_tag="MJE33_002226"
     CDS             complement(10971..11300)
                     /locus_tag="MJE33_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBY9648158.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKHHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            complement(11324..12613)
                     /locus_tag="MJE33_002227"
     CDS             complement(11324..12613)
                     /locus_tag="MJE33_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBY9648159.1"
                     /translation="MEALQNNLRRFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLAPAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            complement(12629..12955)
                     /locus_tag="MJE33_002228"
     CDS             complement(12629..12955)
                     /locus_tag="MJE33_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY9648160.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            complement(13090..13719)
                     /locus_tag="MJE33_002229"
     CDS             complement(13090..13719)
                     /locus_tag="MJE33_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648161.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            complement(13934..16591)
                     /locus_tag="MJE33_002230"
     CDS             complement(13934..16591)
                     /locus_tag="MJE33_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY9648162.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQSELLGIKKGTKVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAMLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERESKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERMELVKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLRYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHGIEYFVFTSLG
                     EEEVINYNDINIINAVNTNLNKSVEMNIADASLFNNVKFKSMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASSMVKAVGDIIGKCSAISFDM
                     KLDKSYDEVIVDFKNLINNIDNKDVLLFTDMGSLNSFDEIIKKEKKCGVRVIPMVTTL
                     TVLEAVQKANMGLPLNDVYNSITNTRKYYFGTNEIQNKENLSKTIIIASHVSEGVDNK
                     TRKILEEKMSRYLDGIDIISVPYKTEKDLSLNITKLKESSNIVAVINEQRINIRGIDY
                     ISKKDIDKDENINKLKNIIKISIGYDDVVEGLKTSLKSSNYNRIFKDIKYVSDELFLV
                     FNIEKKYDKVIGLMMHLAFMVDGLIGNTREIEKLDKEKTLDYHKSLSKIKDIVSQLDK
                     KYNIEINEKECYQILLILEYAEIIEKDYQ"
     gene            complement(16950..17894)
                     /locus_tag="MJE33_002231"
     CDS             complement(16950..17894)
                     /locus_tag="MJE33_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009527644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9648163.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMNKVYEMLKLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRNRIEYNSN
                     GYDCSNEKSLSWIEVSDGHFVYSSKSEINKYQQNLKVV"
     gene            complement(17887..18912)
                     /locus_tag="MJE33_002232"
     CDS             complement(17887..18912)
                     /locus_tag="MJE33_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9648164.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSSDLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDDYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            complement(18987..20555)
                     /locus_tag="MJE33_002233"
     CDS             complement(18987..20555)
                     /locus_tag="MJE33_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBY9648165.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGVLPKHALEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGIFWNIADWTIE"
     gene            complement(20668..21579)
                     /locus_tag="MJE33_002234"
     CDS             complement(20668..21579)
                     /locus_tag="MJE33_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9648166.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIKNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            complement(21563..22549)
                     /locus_tag="MJE33_002235"
     CDS             complement(21563..22549)
                     /locus_tag="MJE33_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9648167.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLDEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
     gene            22909..23310
                     /locus_tag="MJE33_002236"
     CDS             22909..23310
                     /locus_tag="MJE33_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBY9648168.1"
                     /translation="MLLKELYSKTMYTVEENFKENGLTHQQIIVIKLIAHNQELTISQ
                     LCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTTGFEFATNFKIKMQE
                     SFDDIFKNCDENELSDLVKNLRNILAKVKER"
     gene            23313..23711
                     /locus_tag="MJE33_002237"
     CDS             23313..23711
                     /locus_tag="MJE33_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03987 family protein"
                     /protein_id="HBY9648169.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMAG"
BASE COUNT         7800 a         4093 c         2715 g         9345 t
ORIGIN      
        1 gggcttataa aagaatcttc tggtcaagta ttattaaata aacataactt gtctaattta
       61 gctgaacaca gaagaactca aatagtaagt agagtttttc aagacccaag tcttggaact
      121 tgcccttcta tgactgttag agaaaacttg tctttagctc ttaacaaagg aaaactatta
      181 aatctaagaa aatgtcttcg ttacaaaaga gatttcttag aaaatttatt ggaaggtgta
      241 tctcttgatt taaaaaaata ccttgatgtt caagtaaaat ttttatcagg aggtcaaaga
      301 caatcattat cactaattat gtcgtgctta acaaatccat cagttttatt acttgatgaa
      361 catacagcag cacttgaccc aaaaacatca aatgaagtaa ttgaattgac taataagatt
      421 gtaagggaaa aaaatataac tacacttatg gttactcaca acttaaaaca cgctcttcat
      481 tatggtgata gattgataat gcttcataag ggtgaagttg tattagatgt aaaaggtaaa
      541 gaaaaagaac aattaacagt tgaagaaatc ttagaaaaat ttgaatatgc agtttaaaat
      601 aatatatcta ttgtattaaa ctcggcttta aaataaagtt aaaattacaa taattatggt
      661 tactttgtaa taattcatat gaatatatga acatatataa caataaattt ttgaggggga
      721 tataaaaatg attaataaaa aaagattggc aagtttgatt ttagctggtg ctcttagtat
      781 atctatgctt acagggtgtt cacaaggagg agactctggt aattcaaaac aagaaagtaa
      841 ttctaaaggt aaagaagtta aaaaaattgg tataactcaa ctggttgaac atccagcatt
      901 agatgccact aggacaggtt ttgtaaaagc attagaaaag aacggattta aagatggaga
      961 aaatattgat atagacttcc aaaatgctca aaatgacatg cctactacac aaagtattgc
     1021 gagtaaattt gcatctgata agaaggactt aatatttgct atatctactc catcagctca
     1081 ggcagcattt aatgctacta aagatatacc tatacttatt actgctgtga gtgaccctgt
     1141 tgctgctgga ttagttaaaa ctcttgaaaa gccaggcaca aatgtatctg gaacatcaga
     1201 ttttgtttct gtagacaaag gtttagaact tcttaaaata tttgctccta aggcaaaaac
     1261 tataggagtt atgtacaata ctagtgaagt aaattccaaa gttcaagttg atgctttaaa
     1321 agaatatgca tctaaaaatg gatttaaagt tgttgaaaaa ggtataacta cttcaaatga
     1381 agttaaccaa ggtatttcta gtttagttgg caaaatagac gtattatatg tccctacaga
     1441 taacttagtg gcttcttcta tgcctatagt ctctaaaata gctactgaga ataaaatacc
     1501 tgttatagca gctgaatctg gtccagttga aaaaggtgct cttgcttgtc aaggtatcaa
     1561 ctacgaaaaa ctaggttaca aaacaggtga aatggcagtt aaaattttaa atggcgaatc
     1621 agtatctgat atgcctgtgg ctacatcaga tgatacagat atcattgtta atgaagatat
     1681 attaaaagct ttaggtatgg aaaaaccttc taatgaaaat atttcttatg taaagactaa
     1741 acaagaatag aaagatattt tctatatacg attaatatgc ctgtttctat aaattaattt
     1801 atagaaacag gcatatattt ttattttaat gtattatcta tttaaagatt atatgtaaat
     1861 gctcttaatt tgtttgtata aattttactt gtataaactt tatacaatca tatttattct
     1921 tctattttct ttattactct acatggattt ccaacagcta cagtatttgg tggtatatct
     1981 tttgtgacta cacttcctgc acctattact acattatcac ctatagtaat tcctggtgtt
     2041 atgattactc ccccacctat ccatacatta tcacctattt ttataggaga accatactct
     2101 atgcctgaat ttctaagttg tgcatcaatt ggatgatagg ctgtatagat ttgaacgttt
     2161 ggagctaaca ttacattgtc acctatttca attttacata catctaaaaa aatacaatca
     2221 taatttgcaa agaaattttc gcctacatgt atgttatatc cataatcaca cctaatattt
     2281 tgttctacat ttatttgctt gccaactgaa ccaaatagtt gtcttaaaat atcttctctt
     2341 ttttcatatt catcctctaa agtattatta aacaatctag ttaatttttt acaatactct
     2401 ctttctttta ctaacaattc atcatttgca tagtatcctt tcccagacaa cattttttct
     2461 ttttcagtca tatagtttac tcctttttgt tataattgta tctaaagcaa aataaatatt
     2521 cctgtggaaa atctaaatat attctgtcac ctttcttaaa tctcatttta cttttttcca
     2581 attctatttg aataggtaca cattcgttag ataaatcagt ttttctaaca tatatagtat
     2641 atgtaaaagg attttctaca atgttaatca attcgaaaca taacttttca tttaaatctt
     2701 cattagaatc caagacctta atatgatgct ccctgattcc cacatattct atattatcac
     2761 ctatttctct atttaatgtc aactcgcatc cccagtctgt agcataaata gtatttttat
     2821 ccagtctatt taatttggat atatttttac accctgttat cttagcttct ataaggtact
     2881 ttggagattc aaagagctct tttttaggtc tctttggaag accttctcct ttgttgtaca
     2941 ctataatatc atcacaaact cggtaagctt cttcaatatc atgagtaaca aatagtatat
     3001 ctcccttgta atcttttaac atattcataa gttctttttc catattgctt cttaaatgat
     3061 ggtccaatgc tgagaaaggt tcatctaaaa gtaatatatc tggagatgtt gccaatgctc
     3121 tagctagtgc taccctttgt tgttgaccac cagataattg ccaagggtat ctcccctcaa
     3181 acccatttaa tttaagtata tctaagtacc ttgctactat ttcctttttt tcactcttac
     3241 ttagttttaa aagcccaagt tctatatttt gacttacagt catatgtgga aacaaagcat
     3301 agttttgaaa taaaaatcct acctttctat cttgtgttga taaatttatc ttcttttcag
     3361 aatcaaatag aactctatca tttaatacaa ttttacctct tgttggtttc tcaagccctg
     3421 ctatacattt taatgtcata ctttttccag aaccagattc tcccaaaaac cccagagtac
     3481 ctttttcttg tttaatttct acctttagtt taaaagagga taaatctttt tcaatatcca
     3541 cgtataagct caactatcca cacctctttc ctataatttt ctgctgaaat tccgaccaat
     3601 aatttagtaa taaaatcatt gctatagata ttgctacaat aatcataacc cacatcatag
     3661 ctttattcat atctccgcct tcaacagcaa aaaatatagc tattggcata gtttgagttt
     3721 ttccaggtat atttccagct atcataagtg tagcaccaaa ttctccaaga gctctggcaa
     3781 acgacagcac caatccacca attattccag accatgctaa tggaactgca atcttaaaaa
     3841 atactttcca ttcattaaga cctaatgttc tagcagctga caacatatta ttatcaatct
     3901 gttcaaaagc tgaccttgaa gttctataca tcataggaaa agacaccact actgctgaaa
     3961 ttacagttgc tgtccaacta aatattaaag tcctatcgaa atttaataaa aactttccta
     4021 taaatccatt cttcccacat agtaataata aaaagaaacc tacaactgtt ggtggcaaaa
     4081 ttagtggaag tgtaaatagt ccatctataa gacccttcca cttacctcta tagttagcca
     4141 taatatatga cacaaatatt cctattacaa aagttattat tgttgacaaa atagatgtct
     4201 taagtgatat ccataagggt gaccaatctg ttcccatctt tccctcctta attttaaatg
     4261 gaaatagtga aactaataac tctattaatt tcactatttc cattttatta taatacacaa
     4321 ttattttcaa ttttcattta tttatatata atttatactt aattacacat aatttatttc
     4381 taagtttatt ctactttttt atatccaaat tcttcaaaaa tcttttgtcc tgactcactt
     4441 aataaaaatt cttcaaattt cttagcagca tctacgtttt tactagcttt aattactgaa
     4501 actggatatg taataggcga atgagttttt tcgtctgttt tttcaactac ttttatctta
     4561 tcattattga cagtatcact gaagtaaacg aatccaacat cagcatttcc tgattgcacc
     4621 catgctaaaa cttcttttac atcttttgca aatactagct tatcttttaa cttatctttt
     4681 aaatttaaat tagttaacac ctcatctgca tacttaccag ctggaacact ttcagcttca
     4741 ccaacagcaa ttttcttcac tttatctgta gtcaaatctt tcatacctga tacactactg
     4801 tcagcagaag aaataagaac taaatcattt tttactaaat ctttcatagt atcagaaact
     4861 aataacttct cttcatccaa agctttcatt tgtttttgac ctgctgaaat aaataaatca
     4921 caaggtgctc cttgttctat ttgttgttgt aatgagcctg atgctccata gttaactgta
     4981 agttttacat ttgaatcaac ttttttatat tcttcttcaa tctttgccat tgcttctttt
     5041 aaacttgcag cagccgatat atttaactct actgaatcac tacttttagt tgttttttct
     5101 tgttttccat catctttttt gtcttcatta ctattgcacc caactgtccc caaagttaaa
     5161 catgcaacta atcctaatat tcccaatatt tttttcataa caatctccct ttttgtaaat
     5221 ttgatacaca ttttaacatg ttttattatg ttttatcatg ttttgttata tatagtttaa
     5281 ttatatatac atgataataa attttcaagt aatttattta cactactttt aaatattttg
     5341 ctacattttc ctttttttaa tgttaaaata accttatatg gaggtggatt atggactcat
     5401 tatcatcttt gacacctgtc gaggtagctg aattattaaa gataacaaaa aatactgtat
     5461 atgaattaat caaaagaggt gaacttcctt cttataaagt agggaaaaaa ataagagttg
     5521 atactaaaga tgttgaagaa tatatcaaca gccaaaaaac tggtaaagta aaggatacac
     5581 atttcaacaa taaaaatata gatataaata ataatttcaa aattattatt tctggtcaag
     5641 atgtaatatt agacatttta gctaggtctg ttgaaaaaaa attggatgga ataagtacat
     5701 ttcgctctta tataggtagt tataatgctt tgtatgagtt atataatggc agagtttcta
     5761 tagcatcatc ccatctatgg gatttcgaaa cggatgaata taatagcact tttgttaaaa
     5821 agctattgcc aggaatacca tgtgtactta taaatctagc ttatagaatg caaggtttct
     5881 atgttgctaa aggaaatcct aaagagataa caacctggga ggatttaaca aaaccaagta
     5941 taaccattgt aaatagggaa aaaggttctg gaactagggt tttactagat ggtaaactta
     6001 gacttctaaa ctttaatggt aaacatataa aaggttatga taatgaagaa ttatcacatc
     6061 ttggagttgc aagtactgtt tctcgtggaa tagcagatgt tggtttggga aatgaaaaag
     6121 ctgctttaca agtaaataat atcgacttta ttcctcttca caaagagcgc tatgatttag
     6181 ttattaaaaa agaagactta caaaatccag tttaccaaac tataattaac ataataaact
     6241 ctcctgagtt taaagctgaa cttcaaggtc ttggcggata tgatttaact gatactggaa
     6301 aaactatagc aaaagtttaa gattaaaatc gcaatctatt ttataagatt tataattttt
     6361 cacaaaattt ataacttaaa cacgaaataa attgcgattt tagtgtctca aaaaatttat
     6421 gtctttcatt ttatcatcca atacaaaata taatataatt aattgtgcaa tcatattata
     6481 ttaatatata tttacataat agtatttttc tttaatgtag attaacatat gatatgtttt
     6541 aaacatcaat ccccttgctt tcttatatat atttggcaac acatatgagt ttaataaatt
     6601 atatacaatg ttgctttctt cttgtatttt ttcaaccaac aagttattta taaaattacc
     6661 tttctcattt tcaataatct cactccaatt tatcttaact tttaatatat atcttcccac
     6721 taggtataca cactctacaa taaataataa aaatacaatg cagtcagtac ctgttgtctt
     6781 atataaatta cccgatacta atggctttgc gaagaaatta taaaaattca aagtatcacc
     6841 cgtataatca aaaagtatta atagcactcc tagaactctc aatactttaa tattcaacat
     6901 gtttttatct ttattatttt tgtttttcat atatactacc tctcttttct ttagatttga
     6961 ttgtaatcta atactaactc ttttatttga ggtataaatg aatttaattg gtagttattt
     7021 tcatttttac tagaaattaa ttcacattac taaacaaaac ttatatttaa acataaaaat
     7081 agcctatcta tcttttaata aatacaaaaa taaataagct actaatattc aattaacttt
     7141 tatcacttaa aacattacta tctataaatt taaatatttt attgaagtat actgtactat
     7201 catgttcaaa tccatcaaca tgaccaatac cttctataag ccataggtct tttttatcat
     7261 gtttaactaa atcatacatt ttcttgctat tttgataatt acaaacagta tcttttgttc
     7321 catgtataaa cagtatgggt acattagatt tctcaactga ctcatatggt tttacatcac
     7381 tgtaccaaaa tccagattta aacttattat ataaatctcc acaagtcacc acataattga
     7441 ttaatatatc tggtattcgt ttttctagta ctcccattct aattgcatct ttcatttcag
     7501 aataaggaga atccagtata taaaaatcga ccttatcatc ttttgaattt atctctgaat
     7561 gcatagcagc tgtccctgcc cccatggaaa atccatgcac tcctaatctc ccttcaggaa
     7621 atttattttt tacaaattta actagactat ctaaatcata cctttcataa agaccaaatg
     7681 tgtaattatc tccaccacta ttaccagtat ttctttgatt ataaactaat acattataac
     7741 ctttatctaa atatctatat gctaccttta acatttcata gtaactactt ccaattccat
     7801 gaacaagtat catagtatct cttgtcttta tatttgaagt tataaaaaga ctttcaagct
     7861 tgtaattatt agaactttgt atcattaatt cagatgtctt gtagtttttt aatttctcca
     7921 gtggtttatc ttttctctca gaaaatattt ttactatatc ttcgtttttt attaactgct
     7981 ctaaaccaac tgaactatta tacactaatt ttccaaatat ataagataca attaatagga
     8041 ttaatagaaa cacaattgat ataattataa atttatttct taaactaata caatttttaa
     8101 aactattttt catatttatt taccatacaa ttttcctata ttatattttc taagtaatag
     8161 cataattgat tatactattc ttataatttc tattatacct acttatttat taaaaataca
     8221 aattatattt tattaatttt tactacttgt aaattattat cttatacaat gaatttgtat
     8281 tatataataa gtatttttag gattctgtta cataatatta tttaaatatt cttctatatt
     8341 tctcttcttc ttcttcagta aacacattaa atacaacttt taaatttttt tcaggatttt
     8401 cttttagcca tagtctaact ctacttacag caagattagc tgcctctttt tttggataac
     8461 caaatacacc tgtagaaatt ccacaaaaaa caatattttt tatttcatca atttctttta
     8521 ttgtatttaa acatgattta taacaatgaa gcagttgctt ttcttgctct ttacttggct
     8581 gaccaccaga tacaataggt ccaacagtat gtactacaaa ttttgcaggt aaacaataac
     8641 ctctagttat cttagcatct ccagtatatt ctaaatgccc ttgttttttt attattttgt
     8701 cacaatcttc tctaagtcta ggacctgcgc atgaatgtat ctcattatcc acgcatagat
     8761 gtaatggttg taaacaccct aataacttgt tatttgcagc atttactatt gcatctgctc
     8821 ttaaattcgt tatattacct ctccatatgg caatcccttc ctctacttct tctatatcat
     8881 ttacatctat taactctctt tcttcacatt caatcaacaa taaattatgt aacatattaa
     8941 gtatctctgg agcaatttct tttggtggat atacattcaa aagtcctcta agtatatctc
     9001 taggcttaat ttcatcattt gataaattgt atattttaga aggaacttct tttgaaaaat
     9061 atgcaattaa agtgtttata ttttttcttc tttgttcgtc agttagaggc ttgatagttt
     9121 tatcaaaatc ctcaaataaa tttacatcat cagcataatc tctccacttc atagccatct
     9181 ctccattctt gtattttaga cttaaaaata attatgtatt tatgtacata tttatttatt
     9241 tctataaaca cctacataaa tcctgttaaa agtgtaaatt ttttattatg caacttctct
     9301 ggttatttct attgattgat agtaaattat tgtatctaaa tttaatccaa ctaacaagta
     9361 actaaaccta ctttcctaac tgcaaaatac actatgtttt ccacttgtat ctcatttgta
     9421 gataaataaa gtccctacaa atacattaac taagctatgt actaaactgt aggaacttta
     9481 aaaatatatt attcttaatt aagttttggc caataatttt tatttgcttc tattaaatca
     9541 tctagtaatt cttttgcaac tgttgcacta ggaactgtct tagatagcat aagtgcttgc
     9601 cacaatttat tataagactt ctcaatccaa gcatcaacta ctaatttttc aactgctaat
     9661 tgttgttcta ttaaaccttt ttggaatgtt ggtattttcc ctattgataa tggttcatac
     9721 ccgtcttttc caactataca tggaatttca accatagcat catcttgtat attttctata
     9781 gctccattat tttgaactat caataacata cgctcctgtg tattaaaagc tagagctgta
     9841 gctaaatcca ctataaactc tgcatgtatt cctatcttaa gcccactgcc cttagaatgt
     9901 ccttgctttt ctatcaatgc acatgaacca aaaacttctt tttctcttcc atcaataact
     9961 tcattggcac gagtataatt tacatcagaa tgatttacaa cataatctgg aaatagatag
    10021 tactttaagt aactacttgg tattgtagtt aaatctgttt ttactatttc tttagtaaat
    10081 aaattagttt caaaccaact atctttcttg tcatttttca ttccttcttc accatttata
    10141 aaaccatatt cttttatatg ttctactaat tttggtaaaa gttcatttcc ttttttatct
    10201 ttaattgaag tataccaacc aaagtgattt aatccaaagt atcttatatc catatgttta
    10261 cgagatttga gtcctgcaat ccttgctata ttatgttcca tacctattgg catatcacaa
    10321 atatttatta ccctagaatt aggtctcaat cttctaacag cttccgctat tattgatgct
    10381 ggattagaat aatttaacat ccaacaattt ggagaatact tttccatata atctatgttt
    10441 tcaagaacac cttctataga cctcattcca taagctattc cacctggtcc acaagtttct
    10501 tgaccaacta ctccatgttt tagaggtatc ttttcatcta gttctctcat ttcaagtttt
    10561 ccaactctta tatgagctaa acaaaaatct acatctgtat aagcctcttt agggcatgtt
    10621 gttgctaaat actctatatt agggtccttt tcctttatta gtatttcaca agctttagct
    10681 accttatttt gtctttcttc atcattatca tataatttga ttgctcttaa tggtaaccta
    10741 tctaagttat ctagcaacat taatatgatt tctggggtat atgtacttcc tcctccagct
    10801 attactactg aaaatttttc cataataata tctccttttc tatttaaaaa tttactgtat
    10861 ttataaataa atttataaat actagtactt taaagtaaaa gggagttcat ttattaaatc
    10921 tattgattta ataaactata gccaaacttc aagcactatt tacaagaaat ttaattctta
    10981 ttaattcttg ggtccatcat ctcaaatata tctacaagtt cagatactaa agaatgagca
    11041 aataatgcat ttgaataatg gtcttcagag tgcacaacta aaaaatcagt cccactatct
    11101 tcactattca tagcatttga tagtaattct aaatgatgct ttgatgctgt ttgtattttc
    11161 tctgaggctg aatttaaaag gtgttttgct ttattgtagt cacctttttt tatttcctta
    11221 agagacatca ttacatcact tttagcatct cctgcttctg atattatact aaatactttt
    11281 tcttccattt caggtaccat atccttctcc ttctaatgac ttgttaaaaa tcaaagctat
    11341 catcatctaa tgttgtagct aattctgcat ttttatttaa ttcttccttc tccattatct
    11401 taaagaatgg ataatatatc aaggtcataa gtattatact tgcaaatact aatacgattg
    11461 ccttccaatc taatgtcatc aaaaatgctg caacccctgg aggtaaaaag cctggtggtt
    11521 ctgcaatagg taatcctact aatcctactt ttaatgccat aaaattaagt gctccaacca
    11581 atacaggtcc aaatacaaat ggtacaaaca tatatgggtt cataactatt ggtgttccaa
    11641 atattactgg ctcatttatt ccaaatagtg atggtaaaat cacaactttt cccatactat
    11701 taaaactctt acacttactt cttgacataa ggtacactaa tcccaaagtt atccctgaac
    11761 cagttacttg caagaaacca tagtaaaatc ctattgtaaa cacatatttt gggtctcctc
    11821 ctgctgctat cagtgttgca ttttctgcca tatatgtagc tgctattggc atccatatac
    11881 ttgaagttat tgctgctcca tgaagcccaa agaaccataa taattgagtt ataaatagta
    11941 ttatagttac tgcaaaaaca ttatctacac tgcttatagc tggtgctaag aaattcatta
    12001 taatagctgg tagtggttga cctgagacat ttttagttat tgctgctatt gctgtaaata
    12061 acattataca tactatcatt ggagttaagt ttctaaaact tttatttact acactaggta
    12121 cttcttttgg aaatctaatt acgagatttt tatcattcat aaacttagta atatcaacta
    12181 ctaaaagtgc tactaatata cttacaaata aaccttgtgc atctaaataa cttgtatcta
    12241 atacaccatc tactacttgt ccacatacaa caagttgtgc aacaactgct gtcataccta
    12301 tttctacatg attaagatta tattttttag ctagtgcctg agctataaaa aatgctgaat
    12361 atatagataa taaactcata gtaaatttat tagggataag taatgtatcc atattttcag
    12421 ataataatgt ttttgcactt ccttcaggta acatattgtt taatgccata aataacatac
    12481 ttaaagaacc aacaattatt attggggtaa tgcttatcat accttctttt atagcttgaa
    12541 gatgcctttg tttttcaatc ttttgtgcta ttggaagaag aaatcttctt aaattatttt
    12601 gaagtgcttc catttttatt ccccccaatt atctattttt atataattcg attgccgact
    12661 tcataacaga aggagcatcc atacgaccat aatgcttagt atctattaaa ccaactgcta
    12721 ttttgtaatt cttacaagct tcaactacat catcttcctt aaacctaatt tgtggtgcta
    12781 caagtactac atctgtatca ttagcttcaa tgtatgattt taaattttca aatgatgttg
    12841 cctctatatt tatagtttta gtttttaact tttcatgctc actacaaaaa cttttcatct
    12901 tttccacaag tacacctgtt gaagctccat ttgcacatac taataaaata ttcataatta
    12961 ccctcctaca atatctaacc tcttttgttt tctaattttt tatcagccaa ttcttactct
    13021 aattcctatc agtttttact ttaattctta ttattttatt ttaattctta ttaatattta
    13081 ctctaattct tagtactgcc aatgattaaa cttgaaagcc gtatctataa cttcttcttg
    13141 cttatatttt tctaatattt ttaagtatct ttctttataa tcattatcta cttctttttc
    13201 tggtattact atactagctt catttaaaaa gtggaatgaa tcccttccca tttctaatcc
    13261 tctatatgta tgcttgtcaa gtgccacgtc cataacttct ggaactttac ccattgcaaa
    13321 tccttttata attatatttt ttaataaatc acttgatcta tctttctcac atgcacaaag
    13381 atatcttatt gcatgtatga aaaataaagg tctgtctcca tctgcatatg aatattcctt
    13441 tcttacttga tttaaattat ttatgattat tacagctgat gggtccccca ttcctatatc
    13501 ttcaactgat attgtaagta tccttctcca tagcttatct tctaattctg gtgatgatat
    13561 atatagttca taagcaaatt cacaagcttc ttccaccata tttcttctta tagctttttg
    13621 aaatgacgat attatttcgt ctgcactaaa tccgtttctt gtagtcattc ttgcccatgg
    13681 gtcatttgcc tttttatctc ttaatatttt atcttgcata ttatttactc ctttttattt
    13741 aaattttaat tatttatatt gctcatttaa taatataagc aagtattatg ccagtttttt
    13801 tagacacttt aaaacactgt tatttcaatc atttttatca tcaaaactta gatactttgt
    13861 ctaagtatat ttatatgtgt ctaatattaa tattatttta gactaaaaaa aattaccaaa
    13921 taagttttta tactcattgg taatcttttt cgattatttc tgcatattct aatattaata
    13981 atatttgata gcactctttt tcattaattt ctatattata ttttttatct aattgagata
    14041 ctatatcttt tattttgctt agactcttgt gataatcaag tgttttttct ttatcaagct
    14101 tttcaatttc tctagtattt ccaatcaacc catctaccat gaaagctaaa tgcatcataa
    14161 gtccaattac tttatcgtat ttcttttcta tattaaatac taaaaacaat tcatcagaca
    14221 catattttat atctttaaat atcctattat aattagagct ttttaaggat gtctttaaac
    14281 cttcaactac atcatcataa cctatactta tctttataat attttttaat ttattgatat
    14341 tttcatcctt atctatatct tttttgctaa tatagtcaat tcctctaata ttaattcttt
    14401 gttcattgat tactgcaaca atattactac tttctttaag tttcgtaata tttaaactta
    14461 aatctttttc tgttttatat gggacagata taatatctat tccatctaag tacctactca
    14521 tcttttcttc taatattttt cttgttttat tatctacacc ttctgaaaca tgtgatgcta
    14581 taataattgt tttagacaga ttttctttat tttgaatttc attagttcca aaatagtact
    14641 ttctcgtgtt agttatagag ttgtatacgt catttaaagg taatcccata ttggccttct
    14701 gaactgcttc taacacagtt aatgtcgtca ccataggaat tactcttact ccacactttt
    14761 tttctttctt tataatttca tcaaaagagt ttaatgaacc catatctgta aatagtaata
    14821 catctttatt gtctatatta ttaattaaat ttttaaaatc tacaattact tcatcatagc
    14881 ttttatctaa cttcatatcg aaagaaatag cactgcactt tccaataata tcaccaactg
    14941 cttttaccat actacttgct gtagtttctc catgcatagc aactataata ttaatgttat
    15001 tcactatttt attttgttca tcttgtttag cttgtactat gaataatgta atatatgcta
    15061 cagtttctat agataaaaat aaatcaaatt cattttcaat tattccaact atttttaatg
    15121 ctgttttaaa ttcacttctg ttatttcttc taatttgatt caaatctatg ttgtttgtag
    15181 agctactttt atccaatacg ctttttatat aaattgccat tgacttaaat attgattcac
    15241 ttatgtctat ttcttcatct tctttaataa tttgtcttat tgattcacac atgcttttaa
    15301 acttaacatt attaaataga gaagcatctg ctatattcat ttctacagat ttattaagat
    15361 tagtatttac tgcattaata atatttatat cattgtagtt aataacttct tcttctccta
    15421 atgacgtaaa tacaaaatat tctattccat gtgttataac ttcatctgct ttaatagact
    15481 tctctctata atcatcactc acttcattta tagatttatc aaaatatttt ttctctatat
    15541 aaactggttc tttattatct cttttatatc ttagatatcc atttgcaaca gctaatttta
    15601 tatcattttg aagttgacct acattatttt tacaatcata aagtaatagt gatttaatta
    15661 tttctttatg tacttttatt gaaatagaaa tactttttga ttcttccttg aaaaaatttt
    15721 tgactaattc cattctttca atcattgtcc tatctctcaa tgatggtatt tctattttca
    15781 ttggtattct tctattgaat gtatctaata gtgttgatgt cggattttct gtagtagcac
    15841 atattattaa taccttagac tctctttctt tcgttgcatc tcctagtctt ttaaatgttc
    15901 ccttatctat aaaagtaaac aacatttctt gaccttctgc tggtagtcta tgaatttcat
    15961 ctaaaaatag tatccctcca tttgcaattt ccaaaagacc ttttctggaa gattctgcac
    16021 ctgtataagc tccttgttct accccaaaaa tatgtcccat aagtaattgt gcattattag
    16081 cataatcagc acaattaaat gttacaaaag gagctttatt ttttctaatt ccactatcta
    16141 tagaataatc gtacatatat ctagcaatca tactctttcc tactcctgtt tctccataga
    16201 taatagtatg aagtccattt gtaggataga gcattgcgac cttgcatttt tctattatag
    16261 gtcttaaact atgttcttta cctattagtg tttcaaaact atcttttttt attttgtttt
    16321 ctacttttgt tccttttttt atgccaagca actcactttg tgtatcaagc aattcattct
    16381 tgttaaggtt aagatttgca gggatataat acacaggtct tccttttatc ttcgtaacca
    16441 taccctcatt gaataattgg tttaaatatg tacttatatt gtttcttgca attcctgtct
    16501 tttctgataa ttggttagct gtaatccctt tttcattttc cctatatgat gattctaaaa
    16561 ctttcattac tttatctttt ttatatatca ttacaagctc ccctacatta tagtatttta
    16621 ttaactaaaa cccctataca cactttattt tttaataaac tcttattctt taactaaggt
    16681 tataatttat caaattttcc ttactttaaa ttatagaaaa ttattgtaga aaaaataaga
    16741 ttattgctca ttttttctac attactattt ttaattttat agtactttct taacaacata
    16801 ctttacttgt ttttaatttt attatatttg aatttattat atttgaaaag tagaaataaa
    16861 aaaccctatt ttggagtagt tttaaaattt ctccaaaaat agggttttag tttgtatttc
    16921 aaataaatac ctgttcaagt atttttatac taaacaactt ttaaattttg ttgatattta
    16981 tttatttctg atttagaaga atatacaaaa tgtccatctg aaacctcaat ccaacttaat
    17041 gacttttcat ttgaacaatc atatccatta gaattatact ctattctatt tcttgactta
    17101 gcatatctag ggtcaggaac aggaattgca gaaagtagtg attttgtata tggatgaata
    17161 ggattattat acaattcatt tgaatctgcc aattctacta attttccatt atacataact
    17221 cctatacggt cactaatatg tctcaccata gataaatcat gagctataaa taaacaagtt
    17281 aaattctttt cttgttgcaa ttttttaaat aaatttacta tttgtgcttg tatagacaca
    17341 tccaaagaag ctataggctc atcagctatt ataaattctg ggtctactga aagagctcgt
    17401 gctatgccaa tacgctgtct ttgtccacct gaaaaatcag atggatactt atttgcataa
    17461 cttctatcaa gtcctactag ctttagcatc tcatatactt tattcattct atctgtttta
    17521 tttttacaga taccttgaat ttttagtggc tctgaaatta tatctgcaat tgtcatacgt
    17581 ggatttaatg atgaagtaga atcttgaaaa ataatctgca tacttttgtt tacatctttt
    17641 tttataaatt tgtacgtttt tttatctgat atgcaatttc ctttgtaaat tacttctcca
    17701 ccagtagatt catgtagtct tattatagtt ttaccagttg tagattttcc tgaacccgac
    17761 tctcctacca atccaaatat ctcaccctta tatatatcaa aacttatatc atcaactgct
    17821 tttacagtag tacttttgtc caaatgaaaa tattgcttta agttttttat ttccagtatt
    17881 ttttctctat tcattagaaa tcacccttcc acaatttact ctaactggta catctacttc
    17941 aggtgcatct gggtgcaata accaagttgc tgcactatgt gtatcattaa ttttaaacat
    18001 tggtggctct ttttcataat ctatcttaag tgcattttta ttccttattg caaaagcatc
    18061 tccttttggt gggttcaata aatttggagg cattccaggt atattatata aatagtcatc
    18121 ctgcgaatct aaagtaggta atgaacccaa caacccccaa gtatatggat gtctagggtc
    18181 ataaaatata tcctctacag ttcctatttc tacaatcttt cctgcataca taactgctat
    18241 tctatcagct atagttgcaa ctactcctaa atcatgagtt ataaatatta ttgacaaacc
    18301 tattttatgt tgtaaatcct ttattaaatc aattatttga gcttgtatag ttacatctaa
    18361 agcagttgta ggttcatcag caatcaatac atctggatta caggcaagtg ctatagctat
    18421 tactatacgt tgtctcattc cacctgaaaa atgatgtgga aattgtttaa atcttttctc
    18481 aggattatca attccaacca attcaataag ctctatggct ctcttttttg cttcactttt
    18541 acttatacct tgatgaatga ttacagcttc tgcaatttgc tttcctattg atatagttgg
    18601 attgagagat gtcatagggt cttgaaatat cattgatata ttctttcctc ttatatcttc
    18661 catctctttt tcagacttct taaccaaatc acttgattta agtaaaacct ctccattttt
    18721 aatatatcca ttgtatggga gtatcctcat caaactctta cacagtacag atttcccaca
    18781 accagattct ccaactatag ccaatgtctc accttttttt agattaaaag atacatttct
    18841 tacagcctga acttctcctt cttctacttt aaaacttaca gatagattat taacctccaa
    18901 taaatgttcc aaaaaccatc aacccctatg ctattcaaac cttagggata tatttttatc
    18961 cctaaggttt ctaattaatt tcagtattat tcaattgtcc agtcagctat gttccagaat
    19021 atacctacac catgatgacc taaaactgta tcaggtgtta atccttttat gtttggtttt
    19081 ccaacatata ttgcatctat ataagcaata aatgtataag gcatatcctt agtcatttct
    19141 acttggaatt gtttatataa ctttaatttt tcatctttat cttctgtttc tcttgctttt
    19201 tgaagtattt tatctatagt aggattagag taagcactgt aatttgcacc tttatctgtt
    19261 ccaaatactt tatatgtatg gtcatctggg tcaaatggac ttccccaacc tattaaatga
    19321 gcatcttgat tagcccaatc tgtttcagtt acaacaacag ctttagcatc aacaccaatt
    19381 tcttttaact gttgtgcaca tattttagcc atatcaactc taacttggtc actctcacct
    19441 gctgttatct cgaaagctaa cttagtacct tctttttcat agattccatc agaacctaat
    19501 ttccaaccta gcttttcaat ttcttgttta gctttttctg gattatattc aaacttttct
    19561 atatcaggat tattatatgg tcccatttgt aaaggtgaat atgctggtac accatgtcct
    19621 aataatacac tgtcaactat tgcttttcta tctattgcat agcttaaagc atttggtaat
    19681 cctttagctt ttttatcttt aaagaattta gaattaaaat tgtataatat acctctatag
    19741 tcggctgttt tcattatatt tactttaaaa ttcttttcgt ctttttcaaa gttagacata
    19801 tctttaggag ttatttgagc taggtctaat tctcctgatt ttaattgcat tgccttagcc
    19861 ttatcatctg gaacaatctt aaagactact ttatctaatc ctggttcttt tacaaaataa
    19921 tcactattct taacaagtgt tatactttga cctttgtccc atttttcaag tttaaatggt
    19981 ccagtaccta ttggtttttg attaaattca tcagtagcta tatccttacc ttccaaagca
    20041 tgttttggta atactcctac tgttaaataa tcaagcattg ctgtatttgg agcttttaaa
    20101 gtaattttta tagtattatc attaaccaca tcaatttttg ttatatcttc ataatttgat
    20161 gctatctcag aagcattatc tggattcatt atagtttcta ttgtaaactt aacatcattt
    20221 gctgtaaatt tctctccatc atgccactta acatcatctc ttaaattaaa agtatatgta
    20281 tttgttgcct catcaaattt ccagtccttt gctagacaag gaaccacctt attattttca
    20341 tcatgagctg ttaatccatt aaatattaac gaatttattt ctccatgttc gtatagtgca
    20401 ggatttatac ttgtatagtc attacttcca taaacaagca cctttccatc tttaggcgta
    20461 tctgccttct tatctttgtc tcctccacta gaacaacctg ccattagact aagtatcatt
    20521 actaaactta aaacttttaa cttctttaat ttcatgatgc tccccctttt gctttttata
    20581 aaatattttt caaagctttg accccattta tttaaataga tatttcaaat acctcagtat
    20641 ttgcttattt aatactcaaa tgctgattta taaattacta gattttttat tattattctt
    20701 tcttatataa ttacctatat ttgttataca caccaatgtg gtaaccaaaa atatacccgg
    20761 aatcagtata atccaccatc tatttgaaag taatgcttcc tctgacaaag ataacatact
    20821 tccccaagaa acaatttcta ttggtaaacc aattcctaaa aagctaagtg tcgattcagt
    20881 accaatagct gcgcctatgt ttgttacaac catgaacatt attgatgcca caaaatttgg
    20941 aagtaaatgt tgctttaata catagaagaa tcctcctccc atgctcttag ctgctagtat
    21001 gtattcgcta ttttttattt gtctaacttc tgttctcact atcttagata tattcatcca
    21061 acttgttatc cctattacta tggacataga tactggattt gaatttccaa gtatagcttg
    21121 tacaaatatt ataattaata ttgaaggtat actaagtatg atttctgtaa acctcatcat
    21181 tgcatcatct acaagttcag atgctgaacc acttatacta ccataaacaa ttcctataac
    21241 agttgatatt acagtggaaa atagtcctat aaataaagat atttttcctc cataccatat
    21301 catagaatat atatctctac ccattgagtc tgttccaaat aaaaaagttt tatttggagc
    21361 taaattagaa cttgctaaat ccatgtatgt tggttcatgt gtcattataa aagatgagaa
    21421 tacacttcca atgactatga ttgataaaat aataattgat atatatggta attctttaat
    21481 ttttttagat aaggttttct ttgtttctat atgttcatct tctgctacta aaatataatt
    21541 ttctcctaca atttcaaaat cactatattg tggcttcatt agtatcacct ctatcatatt
    21601 tcattctagg gtctatttta ttgtttataa tttgagctaa catattccca aatactacta
    21661 aagcccctgt tattaagcat aaaactaata acatattata atcatgatac tttgcacttt
    21721 caaagcaaag tgttcctaac ccaggataag aaaaaacttt ttcaactaca taagttcctc
    21781 ctagtatatg aggtatcgaa atggccataa tactaatata tgatggcatt atatttctca
    21841 ggcaatgctt aaaaacaata gttctattat ttaaaccttt tgctttacat aaaagcacat
    21901 aatcttctct tatttcctct agtaatttat ttcttatcat atatgtgtaa taccatagat
    21961 ggcttaatat aagtactgta agaggtaaga ttagatgtga tatcctactt aatatactgg
    22021 actcttctcc cattgaataa gcaccactac ttggaagtat acttaagttt atactaaata
    22081 taagtatgag tactaaggca acccaaaatg atggaataca gttggttatt gttcctaatt
    22141 tgcatatgat tctatcaatg agcttatctt catgtaatgt acaaaatatt cctaacaata
    22201 aggctagcac aaaggtaagt atatatcctg aaccacctaa tattatagtg tttatataga
    22261 catcatttat aacagaagta acattttgtt tatatttaaa tgaaattcca aattcccctt
    22321 ttgatgcatt tgaaatccat tttatatatt gactgtatat tggttcatcc aaaccaagtt
    22381 ttttcatagc attttctttt tcttgtgtac tcattctttc tactccatcc ccatagtaag
    22441 agattaatgg gtctcctgga gcaagtcttg ccatgtaaaa aacaataaaa gataataaaa
    22501 acattactaa aataaactgc aacaactttt tcagtatatc ctttaacaaa ccccaacccc
    22561 cctttgttgc aataaaaaaa accacataaa agcaaacgta aagtaacgtt aaaaccttcg
    22621 tggttccatt atcattaaac aaaattaaat tttctatatt tatctatgag ttaattttag
    22681 catcatttat taactgtgta aacaaacttt ttaaaaattg ttaaaatttc atcaaattat
    22741 tttatttata cctaaaataa aatttttata ttctattctt cattattttc tataaaatta
    22801 taaaatttta ggttgactat ataagaaaag tatattatat tagtttgtat gcaaaccatt
    22861 taagaggtga ttttattgaa aagtaattat gactgtttta gaatagccat gctcttaaaa
    22921 gaactttatt ctaaaactat gtacacagta gaagaaaatt ttaaagaaaa tggattaaca
    22981 caccaacaga ttatagttat aaaactgata gctcataatc aagaacttac aatatcacaa
    23041 ctatgtgatg aaatgtcttt agctaaaggt acggtttcag gcataatcag cagattagaa
    23101 cagattggat atatagaaaa attcaagaaa tctaatgata aaagaaatac atacgttaag
    23161 tttacaacaa caggatttga atttgccaca aattttaaaa ttaaaatgca agaaagtttt
    23221 gatgatattt ttaaaaattg tgatgaaaac gaattgagtg acttagtaaa aaatcttaga
    23281 aatatattgg caaaagtaaa ggagagataa ttatgaatta taaattaata cttgcaatag
    23341 tatttataac atcagcttta attttctaca ctataggtgt atttggagaa agaaaggcaa
    23401 aaattttaaa aaagaaacat gtaattatat tttggcttgg ttttatattt gatactttag
    23461 gaacatttac aatgagtaac atagccaaca gccatacttt tgaagtaaaa tctgctttat
    23521 cacaaaacct tcatagtatt acaggtcttc ttgcaattgt gcttatgctt tttcatgcaa
    23581 gttgggctac ttttgtccta tacaaagatg atgaggaaaa gaaaaaattc tttcataagt
    23641 ttagtatagt tgtatggact atatggttag ttccatattt tataggtatg tttattggta
    23701 tggctggata gtaaaataaa aagtgtgaaa gaaggatgta ggtaaatttg tataagacaa
    23761 atttacctac atccttcttt cacactttta catatttatt tataatactc cattatttct
    23821 ttcatgttcc ttaataccta tatagaatat ttaattgttt ctatagtatt ttattatttt
    23881 ctcacacttt ttcatatata tattgtttta ctatttcttc atatgtatat atgaaatatt
    23941 ccattatttc tat
//
LOCUS       DAGSRM010000051        23777 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.051, whole genome shotgun sequence.
ACCESSION   DAGSRM010000051 DAGSRM010000000
VERSION     DAGSRM010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23777
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.051"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            289..459
                     /locus_tag="MJE33_002238"
     CDS             289..459
                     /locus_tag="MJE33_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648170.1"
                     /translation="MSALLYGVCNKSISVSIVINMQEFKIGVSMAYGVLVIGICYIAL
                     GIIFKLDRKRFT"
     gene            1002..1880
                     /locus_tag="MJE33_002239"
     CDS             1002..1880
                     /locus_tag="MJE33_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBY9648171.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQSANEKNAPV
                     ILAIHPNELSFLGDSFVASCIEEANKSKVPMVIHLDHGENKEQILRAIRCGFTSVMID
                     GSHLPYEENVAISREIVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FVEKTGVETLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCRELLEQNPSLYEPDTIYPPCIKSAREVIEFKMN
                     LFNAIDKLKCFYKNKN"
     gene            1916..3811
                     /locus_tag="MJE33_002240"
     CDS             1916..3811
                     /locus_tag="MJE33_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY9648172.1"
                     /translation="MIGKILRSDCIVLDKQLQTKKDVINYMANHLYHGNYINNKEKFI
                     ADINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDREITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMEENFLKHLLSAETPYEILSIFNNKAAESGDFSNNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQLSRFIGKKVLQKKVVDAKNVSLAKNLIINAENGMAKEIKPGGKTLEDSK
                     EMLQGSEKKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFAPAMIAGFMAVIGGSGFIGGMVAGFATG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSAPVAYIACILIGTVITALIIGVTKPTIRSENS
                     EVELLGA"
     gene            4079..5986
                     /locus_tag="MJE33_002241"
     CDS             4079..5986
                     /locus_tag="MJE33_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBY9648173.1"
                     /translation="MLVSRQIEFLKLILNENKYRPIKYFKDKLNVSDKTLQKDLKMIE
                     KYLETFNIKIDIKRGYGILIEDLAKKNIDLINSLNIQSKENKSLSVEQRRMEILKYLL
                     LNSNNITSINQLADKYYVSKTSIVNDFKYIEAWIKEYNLKLNKTLEGTKIIGKEVDIR
                     KSIAKMMDDLLDENYEQHDISELTRLDSATFSALINLFDIDSIIFVETIITELEYNLG
                     YTINQPYYINLITHILICLKRIEEGNQIESQEEREISVDNLDELVYKNVSTLIDKIEN
                     RYRVKITKDEIMYIYIFLVSSGFSSENNSSYNDESDRLSESEKVSSIMIENMSRFLNI
                     NLKNDDLLQKSLASHVRPMLNRLRYDIQIKNPLLGEIQERFSDVLGLCMMTINIMTEY
                     DNLKNISIDEVSYLATYFQAAIERNMSSKRVIVVCHTGYGTSQLLAARLKREFPEWTI
                     VDIVPMHQLSKRNLDDVDFVISTVNLDLKDKLHIVVSVLLMENDINNIKNALLSKPKK
                     SMNLDTNLLGMYLEDNIYFNFDVAQMESLIGVNLGSKYESISLGKDLNIYIHKQSKLN
                     KGIMNINNVTRTIDIYLEISNQSFLKNILMEISNLYRQKNYINYIIDCKSKEDIKILF
                     KNNLLEKTNGN"
     gene            5976..6425
                     /locus_tag="MJE33_002242"
     CDS             5976..6425
                     /locus_tag="MJE33_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY9648174.1"
                     /translation="MEIREILTEESIILDLKSTTKEGIIQELVDKLYDTGSITLKSKF
                     IKALKHREKIGSTSIGYQMAMPHGKTKYVKKASIVIGKSKKGVDWPSIDGLPVKVIIM
                     FAMPQSGGNEDMILISKIAYILGGKNIGERLLNANTKKEILDIFYEV"
     gene            6525..7412
                     /locus_tag="MJE33_002243"
     CDS             6525..7412
                     /locus_tag="MJE33_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBY9648175.1"
                     /translation="MAIYLEMPKLIHEFPFRSLSNEGEVLTTPHWHKEIEILLITEGV
                     VNLFINDKPMQLKKGEIVIIKGGDIHYILPSPGSERLVFQFDISFFNDLILLNEEKES
                     LINLFSSIKKCSRDWSAKIRKSVFDILIDIYNEDLYKIEGYSYAIKGKMYNLIPILYR
                     QIPREIEENTVEYEINSKEILERLNNIFKYVEKNYKQPIKLEDVSYEVGFSVYYFTKF
                     FKKNTGKTFITFLNEYRLEKAKWILLNNNISIVDLVEEVGFGSTKTFYRVFKEKIGVS
                     PLEFKKRYNSLEKKGSVSK"
     gene            7815..9263
                     /locus_tag="MJE33_002244"
     CDS             7815..9263
                     /locus_tag="MJE33_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBY9648176.1"
                     /translation="MKLGKDFLWGGAISANQSEGFYLDGGKGLSQFDKLPLNDKRLKD
                     VCLDEDNILNENNQYYPSHIGIKFCENYKEDIAMLAEIGFKCFRFSISWSRIFPTGEE
                     SEPNEKGLQLYDNIIKELKKYNIEPLITISHFDIPYSLVEKYSGWADRRVVNLYVKYA
                     EILMKRYKDDVKYWIPFNEMNMIMHIPYIGAGLTFIADENQLQKKYQAAHHQLLANAK
                     TIEIGRYINKEFKFGCMLAAGKTYPYTCKPEDVFATLEHERYNLFFSDVQVRGVYPSF
                     MNYYLKSNQIKLIIKEEDLSTLKDNTVDFVSFSYYSSACSSARAEGLEKSKANGPETI
                     KNPYLPKSNSVWQNDPVGLRITMNQLYERYQLPLFIVENGLGTQDIVNEDGKIIDDYR
                     IDYMKEHIKNMILGVNEDGIELIGYLMWGCIDVTSVSEGKMTKRYGIIHVDSDDYGRG
                     SFKRTKKQSAFWYKKVIETDGEILFNDMETHA"
     gene            9289..11679
                     /locus_tag="MJE33_002245"
     CDS             9289..11679
                     /locus_tag="MJE33_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBY9648177.1"
                     /translation="MSLIFTTKDQLIEQGFSKDTIKSNETLFTLANGHLGVRGNIEES
                     DFNNEYVDNMGTYVNGFYEESPITYGESAYGYAKQNQTICKLPNAHIVNFSIEGDYFD
                     LSKGITSEHSRILDLNEGILKRSFIWENSCGRKVKVSIERLVSQDIEELMMISYTITP
                     LNFNGKIKFKNKMENSMEKFNCEKVDDPRVASINKKQFKINVKKYNNKYFMNLVTERS
                     RLGLWCGFDSYITCGNITNETYNVSDECIEREIEVYAKKDERLNLEIVVGYSRVYSDL
                     NLNEERLKSLECLESLKCLESEAHLKSLELVLREACNIGFDKVKNIQRKKMNEFWTTS
                     DVEIEGDKTLQLGIKTNLFHIYQSAGRDGKTNISAKGLTGDGYEGHYFWDTEMYILPF
                     FIYTQPSIARALLDYRYSILDASRERARELGINKGCLYSWRTINGNECSAYYPTGTAQ
                     FHINADIAYGIRTYFEATNDEEFMMQNGLEILIETARFWVEFGDYIESKNNKFCINGV
                     TGPDEYTAIVNNNYYTNLMAKYNLIYAVQMVDKFKHNLFSNEVFRKVKLQEYEVKNWI
                     NAAQNMYLPYDEEKKISKQDDSFLEKSVWDFKNTPKEKYPLLLHYHPLTIYRYQVNKQ
                     ADTVLSALLFPDEFSLEQKKRDFEYYEKITTHDSSLSRSIFGMMASEIGNHEKAYNYF
                     MDTALMDINNLQKNTSDGIHAANMGGTWMSLVYGFAGMKIKNDKLSFEPRIPKHWKNI
                     KFNILFRDNLVSVNIEEYKSYYKLIKGRNITILHDNKEIKLSKNIIEVNNCFKGSN"
     gene            11681..12352
                     /gene="pgmB"
                     /locus_tag="MJE33_002246"
     CDS             11681..12352
                     /gene="pgmB"
                     /locus_tag="MJE33_002246"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBY9648178.1"
                     /translation="MKAVLFDLDGVITDTAKYHFEAWHEIAKSLDIEIDETFNEKLKG
                     ISREESLDKILLSANKVDSYTKEEKLKVCEEKNNIYIKLINSLTYKDILPGINELIKE
                     LKKNNVKLGIASASKNAPRILESLGIKDEFDCIVNPSLLKRGKPYPDIFIEGSKILNL
                     KQYDCVGIEDSYSGIKAINAANMTSIGVGKKEVLGKCDHIVEDTSLLNCNTLLQAWSK
                     KINLD"
     gene            12391..13755
                     /locus_tag="MJE33_002247"
     CDS             12391..13755
                     /locus_tag="MJE33_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBY9648179.1"
                     /translation="MKNKYEEVAQLIIDGLGGKDNIIDLTHCMTRLRFILKDEKKVNA
                     DKLKSIDKVVGVNSTSTQYQVIIGNEVGVVFNAIINKGINTTGDIKSDIKNKKNSILN
                     NIIDTITGCMTPMIPALTASGMIKVILSLCTTFNWLSSNSSTYRVLDIIGDVAFYFMP
                     IILAINAAKKFNVNTSIAVIVVGVFLHPNFSAWVSSGDPISFIGVPIQGVIYVASVIP
                     ALLTVWMMSYIEKFIDKLTPSMLKTILNPTLVLLISAPIALIVIGPIGNFLGEGLASI
                     INLLQGRLGFIMVCLLAAAMPFIVMTGMHHALTPIFISAFATTGQESLILISQVCANL
                     AQGGATLAVALRSKDSKMKQLASAAWISAIMGITEPALYGVTLKLKKPAMLASIGAGI
                     AGLFAGITHVTLYVPQNSIMAVLGLSGEKGISNIVNGLIVIFIALVAPFIMTYVFGVE
                     KNVK"
     gene            14102..14944
                     /locus_tag="MJE33_002248"
     CDS             14102..14944
                     /locus_tag="MJE33_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBY9648180.1"
                     /translation="MIINKILNNNVVITLDDNDEEVIVMGKGIGYQKSKGNLIDKTKV
                     NKVFRISNKEISNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYISLSDHTY
                     SAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGIKLPEDEAGFIAFHI
                     VNAQLSEGHTLASDITKLIQEVLSIVRYHFRIEFHEESVFYYRFIMHLKFFAQRLLLD
                     SAHEGETDKELLSIIKSKYNKEFECVVKIKNFIKKQYNYILTDDELIYLTIHLAKVVK
                     DSNM"
     gene            15089..17017
                     /locus_tag="MJE33_002249"
     CDS             15089..17017
                     /locus_tag="MJE33_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBY9648181.1"
                     /translation="MGKYKQLAQEIVNNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDDSSTSSETKKSGNILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISAGADVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNKCVPNLVKFFFVPMLTLLITVPVALIV
                     IGPIATFGSTLISQIVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMAIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGFFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIA
                     ISMVVAFVLTMILYKDEKVEESKKVRNKGKEEIKEVKSTKLEREIVTSPIKGEVLKLS
                     DIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDTGVEVLIHIG
                     LNTIQLEGRGFEACIKQGDRITKGQTILNFDVYAIKELGYSTVTPIVITNSSQFLDVV
                     ETESKNIELEDNLITVLF"
     gene            17078..18505
                     /gene="ascB"
                     /locus_tag="MJE33_002250"
     CDS             17078..18505
                     /gene="ascB"
                     /locus_tag="MJE33_002250"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBY9648182.1"
                     /translation="MSFKNDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRTAV
                     ITGKLKMFDFDDEHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPKGDE
                     ETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEKLC
                     NVIFRRYKGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVASAI
                     ATKIAHEVDSNNLIGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYPNY
                     ALKYMEQKGITPEMEPGDIELLNKYTVDFISFSYYNSRCVRTDENADDMAEGNIFTSA
                     KNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFIVENGLGAKDIADENGYVEDDYR
                     IDYLREHIKAMHDAVSIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSGNG
                     SLRRMKKKSFEWYKKVIASNGEDLY"
     gene            18952..19728
                     /locus_tag="MJE33_002251"
     CDS             18952..19728
                     /locus_tag="MJE33_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBY9648183.1"
                     /translation="MKYQHIIEVDKELCIGCGLCKNDCPVNNIIIENKKSVIKKQDCL
                     MCGHCAAICPTKAITLTGFDEPPIELTNKPKLDSDELLMAIKSRRSIRKFKDKEVSSE
                     IIKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSKEV
                     EIDDNFFFKHAPIAIMIITKDKISGSLAASNMALMAESYGLGVLFSGYFSDVANNSPK
                     LKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEVATVRYL"
     gene            19744..20109
                     /locus_tag="MJE33_002252"
     CDS             19744..20109
                     /locus_tag="MJE33_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBY9648184.1"
                     /translation="MEKECQQRMQEITSKFKECRKAISAMGDETRQLILIALLESDFN
                     GIRVGEITKKTHLSRPAVSHHLKILKEAEIVNLRREGTKNYYYLDSKKSQWKSLTELI
                     NLIFVGIQHISENDIRKGD"
     gene            complement(20275..21024)
                     /locus_tag="MJE33_002253"
     CDS             complement(20275..21024)
                     /locus_tag="MJE33_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440038.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBY9648185.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELISA
                     DKPFIFVCPTYAWRLPIVVTDFIKETKFLGSNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENEFETITPS
                     GLGGTIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNN
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
     gene            21482..21907
                     /locus_tag="MJE33_002254"
     CDS             21482..21907
                     /locus_tag="MJE33_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY9648186.1"
                     /translation="MINENLKSLRKINQYTQEELAEKLNVSRQSIAKWESGESIPDIC
                     SCIKLAKLYNVKLDDLVNHSEEKTGIIVPPKGKFFFGAVVVGERGQIVIPKEAREVFN
                     INAGDKLLVLGDEERGLGIVHQRDLINFIGHVGVVRKEE"
     gene            21939..23126
                     /locus_tag="MJE33_002255"
     CDS             21939..23126
                     /locus_tag="MJE33_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBY9648187.1"
                     /translation="MSKIVFFSIPAYGHTNPTIEVVRELVDRGNEVLYYSFNEFKDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPGLIEMIVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFAFNKHTAKLMKQNFIEIIRMIFGVRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIE
                     PKERKRKLIYISLGTINNKNVSFYKNCISAFKDSDVDVIMSVGRSTDIKSLGNIPNNF
                     EVKNSVEQIAILQKTDVFVTHSGMNSVNESLYYGVPMVLFPQHSEQRMVAKRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            23318..23722
                     /locus_tag="MJE33_002256"
     CDS             23318..23722
                     /locus_tag="MJE33_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HBY9648188.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNCIKPWKDAETYIDKYMVK
                     QDISKDNIKSITKEKAKKASYEGILYKVYYKDDPRYEYQYFYSDNYYALYVNKVMLQI
                     YDMENNNKLLRSNEELKSVKYPPIYLKYKPDK"
BASE COUNT         9503 a         2460 c         4169 g         7645 t
ORIGIN      
        1 aattgtttta agataaaatg taataaaatt taatggagaa attttatagg tagataaatt
       61 ttaatattta taagattatg ttaaggaaac acttaaaaat tagattaaaa ttgtgggtat
      121 ttaaatcaac taaaagatat tgactattta gtcaatatct ttttttatta aaaaggaata
      181 tacatataaa taggttagat taaaaattgg gataaatatg atttatatag catcatttaa
      241 ttaaaaggag ctatattagt attcattcat acaattggat aatatacaat gtcagccttg
      301 ctttatggtg tttgtaataa atctatttca gtttctattg taattaatat gcaagaattt
      361 aagataggag tatctatggc ttatggtgta ttagtaattg gtatatgcta tatagcatta
      421 ggaataattt ttaaattaga tagaaaaaga tttacataag ataacaaagg ctaatttata
      481 aattggtgct tagaaaacca aatcacttta ttagctagag atgaatttgg agggtgtcac
      541 tttggtgttt tatgtaaagt ttatggaagt aaaacattag ataatcgtga gtaagatttc
      601 tataaatcgt atatactatt ggaatccaag aaaaggtatt ggccaaatag ctaatatctt
      661 tttaattttt ttgactctaa tatagcatta actattgcat gagtatcttt aaatttatag
      721 ttaatgtatc ttaattgttt attataaaat ctaggaaggc taaagcataa aacacttaaa
      781 aagagaagat gtacatgaga aatataatta actatgatgc agacttctaa aaattattta
      841 gtgaaaagga acttagagtg tataataaaa gttccttttc actaaggaaa acgattgtta
      901 ttttctgaca ttcattaccc tgatattatt taaatataaa caacaacaaa attaacaaga
      961 agcaaattca acctaaaatt taaaatatcg ggaggtacaa tatgttaata aacatgaagg
     1021 aaatgctaaa agtggcacaa gaaaatcaat ttgctgtacc agcatttaat ataggaagtg
     1081 ggcaaatact taaagcagta gtacagagtg ctaacgaaaa aaatgcacca gtaatacttg
     1141 caatacaccc aaatgagcta agttttctag gagatagctt tgtagctagt tgcatagaag
     1201 aagcaaataa gagtaaagta cctatggtaa tacacttaga tcatggggaa aataaagaac
     1261 aaatattaag ggcaataaga tgtggattta catcagtaat gattgatggc tcacatttac
     1321 catatgaaga gaatgtagct atatctaggg aaatagtaga aatagcaaaa ggattaaatg
     1381 tgtccgtaga aggagaactt ggaacaatag gaacaacagg aacatcaagt gaaggtggta
     1441 ctgatgaaat aatatataca gattcaaaat tagctaagga ttttgttgaa aaaacaggag
     1501 tagaaacact agctattgca attggtacag cacatggtat atatccaaaa ggttttaaac
     1561 cagagcttaa actagattta ttaaaagaaa taagagaagt agtagatata cctttagttc
     1621 ttcatggtgg ttcatcaaat ccagatgaag aaatagcaca agcagttaaa ctaggagttt
     1681 gtaaaataaa tatttcgagt gatgttaagt cggcatatta taagaagtgc agagaactat
     1741 tagaacaaaa cccaagctta tatgagccag atactatata tccaccatgt ataaaatctg
     1801 caagagaagt tatagagttt aaaatgaatc tattcaatgc tatagataag ttaaagtgct
     1861 tttataaaaa taaaaattaa ttatgaggta acaaattaaa aggaggggag aatttatgat
     1921 aggtaaaata ctacgaagcg attgtattgt actagataaa caattacaaa caaaaaaaga
     1981 tgtaattaat tatatggcaa atcatttgta ccatggaaat tatataaata ataaagaaaa
     2041 gtttatagct gatattaata aacgcgaaaa agaatcatca actaatatgg gtaatttaat
     2101 agctatacct catgctaagt caaatgtagt aaatcaacct gtaatgatgt atttaagaac
     2161 agatagagag attacagata atgatggtca aaaacttagg tcatttttta tgatagcagt
     2221 tccagaatca ggttcagatg aacatcttaa gattatatct aaactagcta catatctaat
     2281 ggaagaaaat tttcttaagc atctactaag tgcagaaact ccatacgaaa tattaagtat
     2341 atttaataat aaagctgcag aatcaggaga ttttagtaat aatcaaaata aaggtcttat
     2401 agttgggata acaggatgtc caacaggtat agctcatact tttatggcaa aaaaagcatt
     2461 agaggaagca ggtcaagaac ttggatatga agttaaaata gaaactcatg gagctatagg
     2521 tgtagaaaat aagttgacta aagaagatat aaaaagagct gaatacgtaa taatagcttg
     2581 tgataaagaa gttcaactga gtagatttat aggaaagaaa gtgctacaaa agaaagtagt
     2641 agatgctaag aatgtttcat tagctaaaaa tttaattata aacgctgaaa atggaatggc
     2701 taaagaaatt aaaccaggtg gaaagacttt agaagattca aaagaaatgc ttcaagggtc
     2761 agagaaaaag ggtgtttata agcacttaat gagtggtgta tctgaaatga ttccatttgt
     2821 agtgataggt ggaattggta tagccatagc ttttatgttt ggtatatttg catcagaccc
     2881 aactcatgaa acatacaacc ctatagctgg attctttgga caacttggag gcgattcagc
     2941 atttaagtta tttataccga tacttgctgg ttttatagct aaatcaatag caggaagaca
     3001 aggatttgct cctgctatga tagctggttt tatggcagtt attggaggtt caggttttat
     3061 aggtggtatg gtagctggtt ttgcaacagg atatatagct ttaggagtaa tgaagataac
     3121 agaaaattta ccagatgtat taaaaggagt aaatgctgta ttgatatgtc cacttttgat
     3181 tgcatttacg agtggagcta taatgttctt tgtagttaat acgccagcat catggttaaa
     3241 tgaagcttta caatcttggt tacaaagtat gtctggaact aataaagtat tattaggtgc
     3301 aatattagca gggatgatgg catcagatat gggaggacct attaataaaa cagcttcagc
     3361 ttttggattg gctatgtttt caagtaatat atttgaacca tcagcagcat taatggtagg
     3421 aggaatggta cctccattag caatatcatt agctatgtta atattcaaaa ataagtttac
     3481 taaagaagaa agaaagtcat ctctaccaac tgctataatg ggggcatctt tcataacaga
     3541 aggcgcaata ccgtttgcag ctgcagaccc attaagaatg attccagcaa atataattgg
     3601 ttcttgtata ggtggggcaa tatgtatgag tttagatata actttaatgg caccacatgg
     3661 gggaatattt gtaataccat ttgcatgtag tgcaccagtt gcatatatag cttgtatatt
     3721 gattggtaca gtaataacag cattgattat aggggtaaca aagcctacta taaggagtga
     3781 aaatagtgaa gttgaattac ttggtgctta aattataaat aatagtaaaa ataattaatg
     3841 gaataacaac aagtatatag aataataata aggatattta gaatcagtta actacttcta
     3901 aatatcctta atttttattt tgaaattata ttatctatta tatcaattta gtaaggtgta
     3961 tcataaattc ttttatgata cagaagtatc ttagtaattt tacatagtta gtaaatatgt
     4021 taaaatagta cttaatataa acatagaaat atataaacat aggtagggag ggctaagtat
     4081 gcttgtgtcg aggcaaattg aatttcttaa attgatacta aacgaaaata aatatagacc
     4141 tataaaatac tttaaagata aattaaatgt atctgataaa actttacaaa aagatttgaa
     4201 gatgattgag aaatatttag aaacttttaa tataaaaatt gatataaaaa gaggctatgg
     4261 aatattaata gaagatttag caaagaagaa tatagactta attaatagtt taaatataca
     4321 atcgaaggaa aataaatctt tatcagtaga gcagagaaga atggagattt taaagtattt
     4381 attactaaat tcaaataata taacttcaat aaatcagtta gcagataaat attatgtaag
     4441 taaaacttct atagtaaatg attttaaata tatagaggca tggataaaag aatacaattt
     4501 aaaacttaat aaaactttag aaggaactaa gattataggt aaagaagtgg atataaggaa
     4561 atcgattgcc aaaatgatgg atgatttatt agatgaaaat tatgaacaac atgatatatc
     4621 tgagttaaca agattagatt ctgctacatt tagtgcattg ataaatttat ttgatattga
     4681 ttctattata tttgtagaaa ctataattac agaattagaa tacaatttag gatatactat
     4741 aaatcagccc tactatataa atttaattac ccatatactt atatgtttaa agagaatcga
     4801 agaaggtaat caaatagaat ctcaggaaga aagagaaatt tctgtagata atttggacga
     4861 gttagtatat aaaaatgtca gtacacttat agataaaata gaaaatagat acagagtaaa
     4921 aataacgaaa gatgaaatta tgtacatata tatttttttg gtatcatctg gatttagtag
     4981 cgaaaataat agtagttaca atgatgaatc agatagatta agtgaaagtg aaaaagtttc
     5041 atctataatg atagagaata tgtcacgatt tttaaatata aatctcaaaa atgatgactt
     5101 actacagaaa agtctagcat ctcatgtaag gcctatgtta aacagattaa gatatgatat
     5161 ccaaataaaa aacccgttgt taggagagat acaagagcga ttttcagatg tgctgggatt
     5221 atgtatgatg actataaata ttatgacaga gtatgataat ctgaagaata ttagtataga
     5281 tgaagtttcg tatcttgcca catattttca agcggcaata gaaagaaata tgagtagtaa
     5341 aagagtaata gtagtttgtc atacaggata tggaacatct cagctccttg cagccagatt
     5401 aaaaagagaa tttcctgaat ggactatagt tgatatagtt cctatgcatc agttgagtaa
     5461 gcgaaattta gatgatgtag actttgtaat atctactgta aacttagatt tgaaagataa
     5521 attacatata gttgtatcag tactattaat ggaaaatgat attaataata taaaaaatgc
     5581 tttactttct aaacctaaaa aaagtatgaa cttagataca aacttattag gaatgtattt
     5641 agaagataat atatatttta attttgatgt agctcagatg gaaagtttaa taggtgtgaa
     5701 tttagggagt aaatatgaat ctatttcatt aggaaaagat ttaaatatat atatacataa
     5761 gcaaagtaaa ttaaataaag ggataatgaa tatcaataat gttactagaa cgattgatat
     5821 ttatttagaa attagtaatc aaagtttttt aaagaatatt ttaatggaaa tatcaaattt
     5881 atataggcaa aagaactata taaactatat aatagattgc aagagcaaag aggatattaa
     5941 aatattattt aagaataatc tattggagaa aacaaatgga aattagagaa atattaacag
     6001 aagaaagcat tattttagat ttgaaatcaa ctacaaaaga aggcataata caggaattag
     6061 tagataaatt atacgataca ggaagtataa ctttaaaaag taagtttata aaggcattaa
     6121 aacatagaga aaaaataggc tctactagca taggttatca aatggccatg ccacatggaa
     6181 aaacaaaata tgtaaagaag gcatccatag taataggtaa atctaaaaaa ggtgtagatt
     6241 ggccaagtat agatggctta cctgtaaagg taataattat gtttgctatg ccacaatctg
     6301 gaggcaatga agatatgata ttaatatcta aaatagctta cattttaggt ggtaaaaata
     6361 taggtgaaag acttttaaat gcaaatacaa aaaaagaaat attagatata ttttatgaag
     6421 tgtaggtatt gtataaatta taaatttgag gtttttaaaa taaaatgtgt attcttttaa
     6481 atagtataaa ttaattttta gacactagag aggagaggat atatatggca atatatttag
     6541 aaatgcctaa attaatacat gaatttcctt ttcgtagtct atcaaatgaa ggagaagtat
     6601 taacaacacc acactggcat aaggaaatag aaatattact tataacagaa ggtgtagtaa
     6661 atctatttat aaatgacaag cctatgcaat taaaaaaagg cgagatagtg ataataaaag
     6721 gaggagatat tcattatata ctgccatcac cgggaagtga aagattagta tttcaatttg
     6781 atatatcttt ttttaatgat ttaattttat taaatgagga aaaagaaagc ttaattaatt
     6841 tattcagtag tataaaaaaa tgtagtagag attggtcagc aaaaataaga aagtcagttt
     6901 tcgatatatt gatagatatc tacaatgaag acctatacaa aatagaagga tatagttatg
     6961 caataaaagg taaaatgtat aatttaattc ctatattata tagacagatt cctagagaaa
     7021 ttgaagaaaa tacagtagaa tatgaaataa actctaaaga gatattagag agattgaaca
     7081 atatattcaa atatgtagaa aaaaattata aacaacctat aaaattagaa gatgtgtctt
     7141 atgaagtagg atttagtgta tattatttta ctaaattttt caaaaaaaat acaggaaaaa
     7201 catttataac ttttttaaat gaatatagat tagaaaaagc aaagtggata ttacttaata
     7261 acaatatatc cattgtagat ttggttgaag aagttggttt tggaagtaca aaaacttttt
     7321 acagagtatt taaagaaaaa attggagtct ctccattaga atttaaaaaa agatataata
     7381 gcttggaaaa aaaagggagt gtctcaaaat gaacttagtt catgaggcac tttcttttat
     7441 atgaaatcaa atatattttc agtatttcaa caatgaaaat tccattcatt tataaagtct
     7501 aaattttagt tctcaataca acctctattt cgaaataaat ccttttatat ataaatatac
     7561 agcaatatat gagaataaga taacaataat gaggatataa aaatgtattt gctatatata
     7621 taattatata catagatatc taatattgat atttaatatg aaagtattga aaatagacaa
     7681 tattaattat agtgagtaaa aaatataatt ggatttttaa attgaattat ttaaaaggta
     7741 tagtatgaaa aaaatcttat aactaaatat gagatagaaa ttataaaact ttatattgtg
     7801 aaagaggtag atgcatgaaa ttagggaaag attttttatg gggaggggca atttcagcca
     7861 atcaaagtga aggattttat ttagatggag gaaaaggttt atctcagttt gataaactcc
     7921 cattaaatga taagagatta aaagatgtat gtttagatga ggataatatt ttaaatgaaa
     7981 ataatcaata ctatccaagt catataggga ttaaattttg tgaaaattac aaagaagata
     8041 tagcaatgtt agcagaaata ggttttaaat gttttagatt ttctatatct tggtcaagaa
     8101 tattcccaac aggagaagaa agtgaaccaa atgaaaaggg attacaactt tatgataata
     8161 taataaaaga attaaaaaaa tataatatag agccattaat aacgataagc cactttgata
     8221 taccttattc tctagttgaa aaatattctg ggtgggctga tagaagagtg gttaatttat
     8281 atgtaaaata tgctgaaatt ttgatgaaaa gatataaaga tgatgtcaaa tactggattc
     8341 cttttaacga aatgaatatg ataatgcata ttccatatat aggtgctggg ttaactttta
     8401 tagctgatga aaatcaactt cagaaaaaat atcaagcagc acaccatcaa ttattagcta
     8461 atgcaaaaac tatagaaata ggaaggtata taaataaaga atttaagttt gggtgtatgt
     8521 tagctgctgg aaaaacatat ccatacacat gtaaaccaga agatgtattt gcaactttag
     8581 agcatgaaag gtataatttg tttttttctg atgttcaagt taggggtgtg tatccaagct
     8641 ttatgaatta ttatttaaaa tcaaatcaaa taaaattaat aataaaagag gaagatttat
     8701 caacacttaa ggataatact gtagattttg tatcttttag ctattattct agtgcttgta
     8761 gttcagctag agctgaaggt ttagaaaaat caaaggcaaa cggaccagaa accataaaaa
     8821 atccatatct tccaaaatca aatagtgtat ggcaaaacga ccctgtaggg ttaagaataa
     8881 ctatgaatca gttatatgaa aggtatcagt tacctttatt tatagtagaa aatggactgg
     8941 gtactcaaga tattgtgaat gaagatggaa aaataattga tgattatcgt atagattaca
     9001 tgaaagaaca tattaaaaat atgattcttg gagtaaatga agatggaata gagcttatag
     9061 gatatcttat gtggggatgt atagatgtaa ccagtgtaag tgaaggaaag atgacaaaac
     9121 gatatggcat aattcatgtt gattctgatg actatggaag aggctcattt aaaagaacta
     9181 aaaaacaaag tgctttttgg tataaaaaag taatagaaac agatggagaa attttattta
     9241 atgacatgga aactcatgca taattcattt taagaaagga gtgttattat gagtttaata
     9301 tttactacta aggaccaact tatagagcaa gggtttagta aggatacaat aaaatccaat
     9361 gaaacgctgt ttacactagc taatggacat ttaggagtta gagggaatat tgaagagtct
     9421 gattttaata atgagtatgt agataatatg ggtacatatg taaatggatt ttatgaagaa
     9481 tctccaatta catatggaga aagcgcttat ggctatgcaa aacaaaatca gactatctgt
     9541 aagttaccta atgctcatat agtaaatttc tctattgaag gagattattt tgatttatca
     9601 aaaggtatta caagtgaaca ttctaggata ctagatttaa atgaagggat tcttaaacgt
     9661 tcatttatat gggaaaactc ttgtggtaga aaagttaaag tatctataga aagattagta
     9721 tcacaagata tagaagaatt aatgatgatt tcatatacaa taacaccatt gaattttaat
     9781 ggaaaaataa aatttaaaaa taaaatggaa aatagtatgg agaagtttaa ttgtgaaaaa
     9841 gtagatgacc caagagtggc atctataaat aagaagcaat ttaaaattaa tgtaaaaaaa
     9901 tataacaata agtattttat gaatttagtt acagaaagaa gtagattagg gttatggtgt
     9961 gggtttgatt catatattac ttgtggaaat attacaaatg agacctataa tgtttcagat
    10021 gaatgtattg agcgagaaat agaagtctat gccaaaaaag atgaaaggtt aaatttagaa
    10081 attgttgtag gctatagtag agtatattct gatttaaatc taaatgaaga acgtttaaaa
    10141 tcattagaat gtctggagtc gttaaaatgt ttagaatcag aagcacattt aaaatcatta
    10201 gaattggttt taagagaagc atgtaatatt ggatttgata aagtcaaaaa tattcagaga
    10261 aagaaaatga atgagttttg gacaacctct gatgtagaga ttgaaggcga taaaactctt
    10321 caactgggga taaaaaccaa tttatttcat atttatcaat cagcaggaag agatggaaag
    10381 actaatatat cagcaaaagg tcttacaggt gatggctatg aggggcatta tttctgggat
    10441 acagaaatgt atatattacc attttttata tatactcaac catccattgc tagagcttta
    10501 ttagattata gatactcaat cttagatgca tcaagagaaa gagctagaga acttggaata
    10561 aataaaggtt gcttatattc atggagaact ataaatggta atgaatgcag tgcatattat
    10621 ccaacaggaa cagcacagtt tcatataaat gcagatatag cttatggtat tagaacgtat
    10681 tttgaagcaa ctaatgatga agaatttatg atgcaaaatg gcttagaaat tcttatagaa
    10741 acagcaagat tttgggttga atttggagat tatatagaat caaaaaataa taaattctgt
    10801 ataaatggag taacaggacc agatgaatat acagcaatag taaacaataa ttactacact
    10861 aatttaatgg ctaagtataa tttaatatat gcagtacaga tggtagataa atttaagcat
    10921 aatttatttt caaatgaagt atttagaaaa gttaaattac aagaatatga agtgaaaaac
    10981 tggataaatg ccgctcaaaa tatgtattta ccatatgatg aagagaagaa aataagcaaa
    11041 caagacgatt catttttaga aaaatcagta tgggatttta aaaatacacc aaaagaaaaa
    11101 tatccattac ttctccatta tcatccatta acaatatata gatatcaagt aaataaacaa
    11161 gcagatactg tactttcagc gttgcttttc cctgatgagt ttagcttaga acaaaagaag
    11221 agagattttg aatactatga aaaaataact actcatgatt catctttgtc aaggtcaata
    11281 tttggaatga tggcaagtga aattggaaat catgaaaaag cttataacta ttttatggac
    11341 acagcattaa tggatattaa taacctacaa aaaaatacaa gtgatggaat acatgctgct
    11401 aatatgggtg gaacatggat gagtttagta tatggatttg caggaatgaa aataaaaaat
    11461 gataaattaa gttttgaacc aaggatacct aaacactgga aaaatattaa atttaatata
    11521 ttatttagag ataatttagt ttctgtaaat atagaagaat ataaatctta ttataagtta
    11581 atcaaaggta gaaatataac catattacat gataataaag aaataaaatt atcaaagaac
    11641 ataatagaag ttaataattg ttttaaaggg agtaattaat atgaaagcag tcttatttga
    11701 tcttgatgga gttataacag atactgctaa gtatcatttt gaagcttggc atgagatagc
    11761 aaaatcgcta gatatagaaa tagatgaaac ttttaatgaa aaattaaaag gcataagcag
    11821 ggaagaatca ttagataaaa tattgttatc tgctaataaa gtagatagtt atactaaaga
    11881 ggaaaagtta aaggtgtgtg aagaaaaaaa taacatttat ataaagttaa taaactcatt
    11941 aacatataaa gatatattac cagggataaa tgagcttata aaagaactta aaaaaaataa
    12001 tgtgaaattg ggaatagcat ctgctagtaa gaatgcacct aggatattag aatctttggg
    12061 aataaaagat gaatttgatt gtatagtaaa tccatcttta ttaaaaaggg gaaaaccata
    12121 tcctgatatt tttattgaag ggagcaagat tctaaattta aaacaatatg attgtgtagg
    12181 aatagaagat tcttattcag gtattaaagc tataaatgct gctaatatga catcaattgg
    12241 tgtggggaaa aaagaagtac taggaaaatg tgaccatatt gtagaagata ctagcttatt
    12301 aaattgcaat acactattac aagcttggag taaaaagatt aatttggatt aaaattatat
    12361 taaaaattat tttaaaagag aggataaggt atgaagaata aatatgaaga agtagctcaa
    12421 ttgataattg atggccttgg aggaaaggat aatataattg acttaactca ttgtatgaca
    12481 cgattaaggt tcatcttaaa agatgaaaag aaagtcaatg cagataaatt aaaatctata
    12541 gataaggtag ttggagtaaa ctcaacttca actcaatatc aggttataat aggtaatgag
    12601 gtaggtgttg tatttaatgc aataataaat aaggggataa ataccacagg agatattaaa
    12661 agcgatataa aaaataaaaa aaatagtatt ttaaataata taatagatac aataactggt
    12721 tgtatgacac ctatgatacc agcccttaca gcatctggga tgataaaagt tatattatct
    12781 ctttgcacca cttttaattg gctatcatca aattcaagta catatagagt attagatata
    12841 ataggagatg tagcatttta ctttatgcct ataatacttg caataaatgc agctaaaaaa
    12901 tttaatgtta atacatcaat agcagttata gtagtaggtg tgtttttaca tccaaatttc
    12961 tctgcatggg tatcaagtgg ggaccctata tcatttatag gagtaccaat tcaaggtgtt
    13021 atatatgtag catctgttat ccctgcactt ttaacagtat ggatgatgtc atatatagaa
    13081 aaattcattg ataaattaac acctagcatg ttaaaaacaa ttttaaatcc aactttagta
    13141 ttattaataa gtgcgccaat agcattaata gtaattggac caataggaaa tttcttagga
    13201 gagggattag ctagtataat aaatttacta caaggaaggt taggatttat aatggtttgt
    13261 ttattagcag ctgcaatgcc atttatcgtt atgacaggga tgcatcatgc attgactcct
    13321 atattcatat cagcatttgc aactacagga caagaatcgc taatattaat atctcaagtt
    13381 tgtgctaatt tagcgcaagg tggcgcaaca ttagctgtag cattaagaag caaagatagt
    13441 aagatgaaac agttagcatc agctgcatgg atatcagcta tcatgggtat aactgaacca
    13501 gctctttatg gagttacatt gaaacttaaa aaacctgcta tgttagcatc tataggagca
    13561 ggaatcgcag ggttatttgc aggaattact catgtgactt tatatgttcc acaaaatagt
    13621 ataatggcag tacttggatt atcaggggaa aaggggattt caaatatagt aaatggactt
    13681 atagttatat ttatagcatt ggttgctcca tttattatga catatgtttt tggagttgaa
    13741 aaaaatgtta aataaagtac agataatgta gtagaggaag tataatatta actatttaca
    13801 ttaatagaat ttttattatt attgactata acttgttttg tatttatatt gatgcaaaga
    13861 aataagttaa tattaataag aataattatt agaactgaca aaaagcaaca taaaagattg
    13921 atgaagtata tggaaataaa aagttgacaa aatatgtctg atgttttatc atattaattg
    13981 gaattaggta gtaattgttt aagtacaagc taagcttatt cattaataat agcagtatcc
    14041 taggaaggag tatgcccatt attatggata agcttttttt atgcttaaaa ggaggataat
    14101 catgataatt aataagatat taaacaataa tgttgtaatc acattagatg ataatgatga
    14161 agaagttatc gttatgggta aaggaattgg ttatcaaaaa tcaaaaggca atctaataga
    14221 taaaactaaa gtaaataaag tatttagaat atcaaataaa gaaatatcaa acaaactaca
    14281 agaattactc aataatattc ctatagaaca catgaaatta tcaagtgaaa ttatagaata
    14341 cgcacaaata aagttaaaca agaagctaaa tgaaagtatt tacatatcct taagtgacca
    14401 tacatattct gcaattcaaa ggatgaaaga aggaataaat gtaaaaaatg caatcctttg
    14461 ggaaattaaa agattttata aagaagagtt tgaaattgga atgaaagcac tagatattat
    14521 tgaaaacaaa acaggtataa aattaccaga agatgaggct ggatttatag cctttcatat
    14581 agtgaatgct cagttaagtg aaggtcatac tttggcatct gatattacga agttaataca
    14641 agaagtactt agcatagtta ggtatcattt taggatagaa tttcatgaag aatcagtgtt
    14701 ttattataga tttataatgc atttaaagtt ttttgcgcaa agattgcttt tagatagtgc
    14761 acatgaaggt gagacagaca aagaattact aagcattatt aaatctaaat ataataaaga
    14821 gtttgaatgt gtagttaaaa ttaaaaattt cattaaaaaa caatataact acatattaac
    14881 agatgatgag cttatatatt taacaataca tttagctaaa gtagttaaag actcaaatat
    14941 gtaagtgaaa taatttttca gtagtatatt aagaatgatt gaaaggagtg atactaacaa
    15001 aattatactt atggttggat ggtaacattg agttggccaa accttcaaat ttcaaaaaat
    15061 taatttaaac gaaatatgga ggagaaaaat gggaaaatac aaacaattag cacaagagat
    15121 agtaaacaat gttggtggaa aagaaaatat tagtgggtta gtacactgta ttacaagatt
    15181 acgttttact ttaaaagatg aaagtatagc aaatgacaat gtattaaaaa acatggaagg
    15241 tattgttact gtaatgaaaa gtggaggaca atatcaagta gttataggaa atcatgttga
    15301 agctgtctat aaagatgtag ttgaaataat tggtttagat gattcaagta caagttcaga
    15361 aactaaaaaa tcaggtaata tactagataa aggtatagat ataatatcag gaatttttca
    15421 gccagtgtta ggaataatgg cagcttgtgg tatgttgaaa ggatttaatg cattatttgt
    15481 agcaatggga ttatatagtg atgtatctgg tggttatatg gtaataaatg cagcaggaga
    15541 tgcattattt acattccttc ctttgttctt aggatatact tcagctaaga agtttggatt
    15601 aaaaccaatg ttgggattag cactaggagc tgcagtatgt tatcctgcta tacaaggtag
    15661 cagtatatca gcaggagcag atgtgatgta cactttattt aaaggaacaa tgtttgcatc
    15721 accagtatat attgatttct ttggactacc aattgtatca attgattata ctggaacagt
    15781 cgtaccaatt atatttatag tgtattttgc ttctaggtgt gaaaaattat ttaataagtg
    15841 tgttccaaat ttagttaagt ttttctttgt gccaatgctt acattattaa taacagtgcc
    15901 tgttgcactt atagttatag gccctatagc aacatttggt tctacactta tttcacaaat
    15961 tgtaatttca ataagagatt ttagtccact attggcaggt gctatagtag gatttacttg
    16021 gcaaattctt gttatttttg gtatgcactg gggatttatt cctgtatata taaacaatgt
    16081 tatgacttta ggatatgata atgttatgat gccattcttt gcctgtacat ttgcaagttc
    16141 ggcagttgtg ttggcaatat ttttcaagac taaagataaa aaactaaaag agatggcaat
    16201 accaaacttt atatctggta tatttggagt tacagagcca gcaatttatg gaatattatt
    16261 gcctttaaag aaaccattta taataagttg tattgctagt ggtattggag gcgctttcta
    16321 tggattcttt aatcttagaa aatttataac tggaggaatg ggaatatttg aacttccagc
    16381 aatgattgaa ccagatgggg gaatgggtaa cttaatagta gctctttctg gtatagctat
    16441 atcaatggtc gtagcttttg tattaactat gattttatat aaagatgaga aagtagaaga
    16501 gtctaaaaaa gtaagaaata agggtaaaga agaaataaaa gaagtcaagt ctactaaatt
    16561 agaaagagaa atagtaacaa gtcctataaa aggtgaagta ttaaaattaa gtgatataga
    16621 ggatgcagca tttgcatctg gtgtactagg tcaaggggtg gcaataatac ctagtgatgg
    16681 aaaagtagtt gctccagtag atggggtagt tacaacctta tttcctagtc tacatgctat
    16741 agggattctt tctgatacag gtgttgaagt gcttattcat attggattaa atactattca
    16801 attagaggga agaggttttg aggcatgtat aaaacaaggt gatagaatta caaaaggtca
    16861 aactatatta aactttgatg tttatgcaat aaaagagttg gggtatagta ctgtaactcc
    16921 aattgttatt acaaattcat cacaattttt agatgtagta gaaacagaaa gtaaaaatat
    16981 tgaattagaa gacaatctaa ttacagtctt attttagtaa agattgaggt attagcataa
    17041 tctagtgctt tggttataat tttaggaggc tttgactatg agttttaaga atgatttttt
    17101 atggggagga gctactgcgg ccaatcaatg tgaaggtgga tacaatgaag acgaaagagg
    17161 attggcaaat gtagatgtat gtccaacagg aaaagataga acagcagtta ttacagggaa
    17221 actaaaaatg tttgattttg atgatgagca ttattatcca gctaaaactg gtattgatat
    17281 gtatcataat tataaagaag atataaagtt atttgctgaa atgggattta aagtatatag
    17341 aatgagcatt gcatggagtc gaatatttcc aaaaggtgat gaagaaacac caaatgaaaa
    17401 gggacttcaa ttttatgaaa atatatttaa ggagtgtaaa aaatataaaa tagagccttt
    17461 agtcactatt acacactttg attgtccaat gcatttagtt aaaaagtatg gtgcatggcg
    17521 taatcgaaaa atgataggtt tctatgagaa attatgcaat gttatcttta gaagatataa
    17581 aggattagtg aagtactggc taactttcaa tgaaataaat atgattttac atgctccatt
    17641 tatgggtgct ggtatttgct ttgaagaagg tgaagatgtt gaaaagataa aatatcaagc
    17701 tgcccatcat gagcttgtag ctagtgcaat tgcaactaaa atagcacatg aagttgattc
    17761 aaataactta attggatgta tgtttgcagc tggaagtgta tatccttata gttgtaatcc
    17821 taatgatgta tgggaagcta caaagttaga tagagaaaat tacttctttg tggatgtaca
    17881 atctaaaggt aaatatccta actatgcgtt aaagtatatg gagcaaaaag gtattacacc
    17941 agaaatggaa cctggtgata ttgaattatt gaataaatat acagtagatt ttatttcatt
    18001 ttcatattac aatagccgtt gtgttagaac cgatgaaaat gcagatgata tggctgaagg
    18061 aaacatattt acatctgcta aaaatccata tctttcatac agtcaatggg gatggccaat
    18121 tgacccatta ggtttgagaa ttacactaaa tcatgtatat gatagatatg aaaagccact
    18181 atttattgta gaaaatggat taggtgctaa agatatagct gatgaaaatg gttatgtaga
    18241 agatgattac agaattgatt atctaagaga acatataaaa gctatgcatg atgcagtttc
    18301 tatagatggg gtcgatttat taggttatac tacatgggca cctattgatt tagtaagtgc
    18361 tggtacaggt gaaatggaaa agcgttatgg atttatatat gtagatagag acaatagtgg
    18421 taatggaagt ctaagaagaa tgaaaaagaa aagttttgaa tggtataaga aagtaattgc
    18481 ttcaaatggt gaagatttat attagacaag aaatctatgt taaataagaa atctatatta
    18541 gacaagaaat ctatgttaaa taagaacttt gtattaaata agaagagtat aaaaattaat
    18601 aataagtata atttttaact tttttaacta tagaaaatca atatagaatt attttacatt
    18661 gtattgatgc aatagtatga ttcacaaata aaattttaga aaataaaatt gttaagaagt
    18721 atgggaagta agtgcaattt actttgtaat aaaagatgaa aaaggcaagc ttaggtttta
    18781 agcttgtctt ttttattatg tataaactat gatattttaa aattctatga aaaatttatt
    18841 taatagagaa attaaaaatt taatggtaat caaattcttt accaatgaat ataaaaaatg
    18901 tctacgtgtt ataatagttt aaactaataa acgaaaggta gatgaatgaa tatgaaatac
    18961 caacatataa tagaagttga caaagaactt tgcattggat gtgggctttg taaaaatgat
    19021 tgtccagtaa acaatataat tatagaaaat aaaaagtcag taattaaaaa acaagattgt
    19081 ctaatgtgtg gacattgtgc agcaatatgt cccacaaaag ctattacctt gacaggattt
    19141 gatgaacctc ctatagaact tacaaataag ccaaagctag attcagatga attactaatg
    19201 gcaattaaat ctagaagaag tatacgtaaa tttaaagata aagaagtttc ttcagaaatt
    19261 ataaagcaaa ttattgaggc aggtaggtat acccctagtg ctaaaaattc acaagatgta
    19321 tcatatattg ttttagacaa taaaaagtca atttatgaaa acgaagcagt gaaatttttt
    19381 agaaaaataa aacctattgc aaatatagct attaaatatt caaaagaagt tgagattgac
    19441 gataactttt tctttaagca tgctcctatt gcaataatga taattacaaa agataagatt
    19501 agtggttcac ttgcagcatc aaatatggca ttaatggcag agtcttatgg tctaggtgtt
    19561 ttatttagtg gatatttttc agatgtagca aataattcgc caaaattaaa aaagctttta
    19621 ggtctaaaac gtagtaatca tgttcttaca acattagtaa ttggataccc agatgttaaa
    19681 tatagacgta ctgcacagaa agaagtagca actgttagat acttataaga gagggtggat
    19741 tttatggaaa aagaatgtca acaaagaatg caagaaataa cgtctaaatt taaagaatgt
    19801 cgcaaagcta tctctgctat gggtgatgag actagacaat tgattttaat tgctctttta
    19861 gagagtgatt ttaatggtat acgagtggga gaaatcacaa aaaaaacaca tctttctcgt
    19921 cctgctgtat ctcatcatct taagatatta aaagaagcag agattgttaa tttgcgtaga
    19981 gaaggaacga aaaattatta ttatcttgat tcaaaaaaat cacaatggaa gtcactaaca
    20041 gagctaataa atcttatatt tgttggaata caacatatta gtgaaaatga tataagaaaa
    20101 ggtgattaaa ttatacttga tttaatacaa aaagattgat ttaggaacta aaaaattcac
    20161 tttgagacac tatcttttga actataccca ttttaggaga cggttaaaat atgtaaaact
    20221 gtataaaaca atgggtatag ttcattttat aatggtttaa atattaagaa tatattattc
    20281 taaatagtat ctttctctgt tttgagtttt atttttatac tcaatagctt cagtggggca
    20341 accacaaata catgccatac aatgagtaca gttatttttc caaactggtt ttttgttttt
    20401 taaattaata ttatttaaag gacatagctc tacacatttt ccacagccaa tgcatttatc
    20461 agtataatga aatcctttgg cactaataat tgtcttataa aaaactgtat ttattatacc
    20521 acttttaatt gtacctccta agccactagg agtaatagtt tcaaattcat tttcattgcg
    20581 tatatcagaa ataatcctat atatttgttt atctgcttct ttaatcattt gttttgatgt
    20641 ttctttgtct gttgtagaaa ataaagctat ataattctct ggcattttaa tttctgccat
    20701 accttttaat tgccattcct tgtatttaca tagtttttga atatagttta tggattttgc
    20761 tgtatctcca ccacaagtca ttacaaagta aactctgttg cttcctagaa attttgtttc
    20821 ttttataaaa tccgtaacta ctattggcaa tctccatgca taagtaggac atacaaatat
    20881 gaatggttta tctgctgata ttaattcatc tgttttttcc tcttttataa gttgatttag
    20941 tgatatcagt tcatcattta attcttgtgc aattttacga gccacatatc ggctattacc
    21001 agttccgcta aaatataaaa tcataataat atctccctaa ctatttttta tttattatag
    21061 taatttattg atatattcta ttcaaatgta tgtaaaattt agtagttaag aaaattataa
    21121 cacattagta atttttatac ttattggtaa agaatttgat taccattaaa tttagcaaca
    21181 agacatcaac tggacttgta tatgagaagg gataataatt tgcttatgaa agattataaa
    21241 tataaaggtt attttaaagg tgtagaaata tttgataatg taaatgacct taacttaaat
    21301 actttaacaa cagttagtag atttgtgctt gtttttttgt ttacgataac ttaaggaaat
    21361 atgttacctg aataataaat ttatataata aaagtatata aatatatata tgaatgttaa
    21421 gattagttga tagaatttaa ataataaatc caatattata aacataggag gtatgaaaaa
    21481 tatgattaat gagaatttaa aaagtctaag aaaaatcaac cagtatacac aagaagaatt
    21541 agctgaaaaa cttaatgttt ctagacaatc aatagcaaaa tgggaaagtg gagaatctat
    21601 tcctgatata tgtagttgca ttaaacttgc aaaattgtac aatgtcaaac tagatgactt
    21661 agttaatcat tcagaagaaa aaacaggtat tatagtacct cctaaaggaa agtttttctt
    21721 tggagcagtt gtagttggtg aacgtggtca gattgtcatt ccaaaagaag caagagaagt
    21781 atttaatata aatgcaggag ataaattatt ggtacttgga gatgaggaaa gaggattagg
    21841 aattgttcat caacgtgatt tgataaattt tataggtcat gtgggagtag tacgtaaaga
    21901 agaatgattt aaaaaaatta taaaaggagg tatgaaatat gagtaaaata gtatttttta
    21961 gcattcctgc atatggtcat actaatccta ctattgaagt agttcgtgaa ttagtggata
    22021 gaggaaatga ggtattgtat tattctttta atgaatttaa agataaaatt gaaggtgctg
    22081 gagcaaaatt tatttgttgt gataaatacc ttccagaatt actccctgga gatgagaaaa
    22141 aaattggtaa ggattttcct ggattaattg agatgatagt agatactact atttctttag
    22201 atgaaaaagt ttgtagagaa cttaaagatt ttaatccaga ttgcattgta tcagattcat
    22261 taagtttttg gggaaagtta tttgcacaaa aacttaatat tccatatgta tgctctacaa
    22321 caacttttgc atttaataaa catacagcaa agttgatgaa acaaaacttt atagaaatta
    22381 tacgtatgat ttttggtgta agacgcatta ataaaaaaat aaagctttta caaagaaaag
    22441 ggtatgaagt aaaaaatttt atttctatag tttctaatga taatgatacg gataccattg
    22501 tatatacatc aaaagaattt caaccaatgg tagaaacctt ctctagtaag tattcatttg
    22561 taggtccatc agtatcaaag ttgattattg aacctaaaga gagaaaaaga aagctaatat
    22621 atatttcgct tggtacaatt aataataaaa atgttagctt ttataaaaac tgtataagtg
    22681 catttaaaga ttctgatgtt gatgtgatta tgtctgttgg tagaagtact gatattaaaa
    22741 gtttaggaaa tataccaaat aattttgagg tcaaaaatag tgttgaacag attgctatac
    22801 ttcaaaaaac tgatgtgttt gttactcatt ctgggatgaa tagtgttaat gaaagtttat
    22861 attatggagt acctatggta ttatttcctc aacattcgga gcagagaatg gtagctaaga
    22921 gagttgttca tttgggagca ggaattatgt taaaagagga taagtcagaa agtattaaaa
    22981 aagcagtatt tcaagttata aatgacaatg aatataaaga aaatgcaact aaactatcaa
    23041 aaagctttta taatgcagga ggctcaaaaa aagcagcaga tgtaatatta cagattatat
    23101 gtaatagtag acaagttgac atataataga tttgttaaaa atatatatac atacatgtaa
    23161 tttaatcaaa gtactgacag tggggtcttt taacctacca gttggggcat atatattgat
    23221 gctaaatatg agggatgtta tattttaata aaaaagataa catccttttt ttagtgcatt
    23281 atatcgagtt ttaaataata aatgaaggag gatcaatatg aaaaaatata aagttatttt
    23341 tatagtatta attataggta ttataagtgt aacagggttt gttgtatata aaaactgcat
    23401 taaaccatgg aaagatgctg aaacatatat tgataagtat atggtaaaac aagatatttc
    23461 aaaagataat ataaaaagta ttacaaaaga aaaagccaag aaggcaagtt acgaaggaat
    23521 actatacaaa gtgtattaca aagatgaccc tcgatatgag tatcaatatt tttatagtga
    23581 taattattat gctctatatg taaataaagt aatgttacaa atttatgata tggaaaataa
    23641 taataaactt ttaagaagta atgaggaatt aaaaagtgta aagtatccac caatatatct
    23701 taaatataag ccagataaat gatttatgac aaaatagaat gatttttatt aaaatttatt
    23761 taaaaatatc actagag
//
LOCUS       DAGSRM010000052        23630 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.052, whole genome shotgun sequence.
ACCESSION   DAGSRM010000052 DAGSRM010000000
VERSION     DAGSRM010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23630
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.052"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..553)
                     /locus_tag="MJE33_002257"
     CDS             complement(<1..553)
                     /locus_tag="MJE33_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBY9648189.1"
                     /translation="MIKTHRVKLNLTRTQFELVREKQMESANCWNYIVNLSKEYYFEH
                     KQWIKKNDIQKLIKGKYNLHSQTIQAISDKFDANRKTISELRKKGNTKAKYPYKTKKF
                     YIIPFKASAINRNAKGNLKLSMSKGRYLELDFNVENIKTAEIVWRNGYYLYYTFDNEL
                     NGVVAKGTNTVGVDLGEIHSIASV"
     gene            complement(550..948)
                     /gene="tnpA"
                     /locus_tag="MJE33_002258"
     CDS             complement(550..948)
                     /gene="tnpA"
                     /locus_tag="MJE33_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBY9648190.1"
                     /translation="MYKSNKNIVYDCKYHIVFCPKYRKPVLVGEVEKMLKEILPYKAD
                     ELGAEIIEMETDKEHVHLLISCDPQYGIHKVVKGLKGFSSRVLRENFPHLKSSMPSMW
                     TNSYFVGTVGSVSLEVVKQYIENQQVRTKK"
     gene            1204..2640
                     /locus_tag="MJE33_002259"
     CDS             1204..2640
                     /locus_tag="MJE33_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBY9648191.1"
                     /translation="MICTIKPLKISIKKSTEPLIEFISDTTYKNKSSSYIKEVYFTRY
                     IGVDDSIDKYIEKIKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYNSSK
                     FNSWDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWI
                     DYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLT
                     SKKIVQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESSLVKIFANNFIEIVN"
     gene            complement(2732..4606)
                     /gene="asnB"
                     /locus_tag="MJE33_002260"
     CDS             complement(2732..4606)
                     /gene="asnB"
                     /locus_tag="MJE33_002260"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBY9648192.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEHVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            4809..5483
                     /locus_tag="MJE33_002261"
     CDS             4809..5483
                     /locus_tag="MJE33_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBY9648193.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLIATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            5596..6132
                     /locus_tag="MJE33_002262"
     CDS             5596..6132
                     /locus_tag="MJE33_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBY9648194.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(6298..6918)
                     /locus_tag="MJE33_002263"
     CDS             complement(6298..6918)
                     /locus_tag="MJE33_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBY9648195.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(7232..9370)
                     /gene="vanT"
                     /locus_tag="MJE33_002264"
     CDS             complement(7232..9370)
                     /gene="vanT"
                     /locus_tag="MJE33_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HBY9648196.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKHLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVNMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINDCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLAE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            complement(9388..10194)
                     /locus_tag="MJE33_002265"
     CDS             complement(9388..10194)
                     /locus_tag="MJE33_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBY9648197.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            complement(10191..11291)
                     /gene="vanG"
                     /locus_tag="MJE33_002266"
     CDS             complement(10191..11291)
                     /gene="vanG"
                     /locus_tag="MJE33_002266"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HBY9648198.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            complement(11552..12634)
                     /locus_tag="MJE33_002267"
     CDS             complement(11552..12634)
                     /locus_tag="MJE33_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBY9648199.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFMNHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            complement(12684..13385)
                     /locus_tag="MJE33_002268"
     CDS             complement(12684..13385)
                     /locus_tag="MJE33_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBY9648200.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            complement(13819..16350)
                     /locus_tag="MJE33_002269"
     CDS             complement(13819..16350)
                     /locus_tag="MJE33_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBY9648201.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            complement(16458..17075)
                     /locus_tag="MJE33_002270"
     CDS             complement(16458..17075)
                     /locus_tag="MJE33_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBY9648202.1"
                     /translation="MCKKTYLLVVSILIAGLLTACSGSPTTDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIMTPQQAMEIALKEQNG
                     IVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            complement(17436..18320)
                     /locus_tag="MJE33_002271"
     CDS             complement(17436..18320)
                     /locus_tag="MJE33_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBY9648203.1"
                     /translation="MDTTKIDDISKLDLNNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLVLSKRDIDLTLRRFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYVEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAIKFATRVWCKTEDYWTIYLDLLEEVKVRFDEEG
                     ITIPYPKMDLTVKELNKI"
     gene            complement(18511..19761)
                     /locus_tag="MJE33_002272"
     CDS             complement(18511..19761)
                     /locus_tag="MJE33_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY9648204.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLANELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKIQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGVVIGCFMYIIVRDTPKEYGFNVDTEPYENSEKVNIVDGIKSVIK
                     NKSTWYNSMIMFSFVGLTSAFISLWGVRYIMDVYGVSKSFSAFIVSFFTYGFIFGSII
                     MDFVFAKIRSSKFNIIKFGAMIDLFIWIVIVVVYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDAKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNIVNGYKKGFVV
                     FIVLSIITIVSSHIGVKNDDFKAI"
     gene            complement(19983..20747)
                     /locus_tag="MJE33_002273"
     CDS             complement(19983..20747)
                     /locus_tag="MJE33_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9648205.1"
                     /translation="MLGKLLKYELKASGRIFIPLYIAILIVAVFNGIFMNTNILQVQG
                     IGILVLTSLFMALGVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVLSFIVAVFTFGVLMLFGTSGILLPEILDLFNTSFKWISENFLDILLLVVVMFISYS
                     SFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPNEDTNMVYH
                     LYQSPSFMLAILGSLVVAIALFFATKFILDKKLNLE"
     gene            complement(20741..21466)
                     /locus_tag="MJE33_002274"
     CDS             complement(20741..21466)
                     /locus_tag="MJE33_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9648206.1"
                     /translation="MDKNISNVSQDGIVEFKGVNKSYGTKNVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGMKPSIDTKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEIIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            complement(21468..21836)
                     /locus_tag="MJE33_002275"
     CDS             complement(21468..21836)
                     /locus_tag="MJE33_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY9648207.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKITLEK
                     LGYDRDEMLKLITENLKGEL"
     gene            complement(22012..23556)
                     /locus_tag="MJE33_002276"
     CDS             complement(22012..23556)
                     /locus_tag="MJE33_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBY9648208.1"
                     /translation="MRNLQKKLMFFLFICIILQITYTTPVYSLCKLYKNNSINNFESI
                     VSNNQLISKHAIQIILIMAIICVILIFYIIYDKLNFKIKLQRIAYTDNLTGANTIDKF
                     VIDANKILCKNTQVKYALLYIDIDKFKYINDLFGYEVGNEILRNLTKIIKRNIFEEEM
                     FARISADNFIIIMKYIEEEDITKRLKTIFEELDLFNNNQEEKYKLVLSCGIYFILPED
                     RDINSIIDRANIPHKMAKGGHKSSYAFYDNKIHDQEIKEKEMENTMFSSLENKEFIIY
                     LQPKIELNTGEIQGSEALVRWKRPDKGLIPPNEFIPFFERNGFVINLDLYVLEEVCIY
                     LRKWIDAGINPVTVSVNVSRIHLYCNNFIETYKNIIDKYNIPAKYIELELTESIIFDN
                     FDILIDIMNNLKKIGFLISMDDFGSGYSSLNMLKEIPMDILKLDQKFIMETYNSKRSK
                     IIVTKVIEMAKELGMKVISEGVETEEQFKLLKEVKCDMAQGYLFGKPMPIEEFEHLIV
                     SNLIRG"
BASE COUNT         8095 a         3985 c         2484 g         9066 t
ORIGIN      
        1 ttacactagc tatcgaatgt atttccccta agtctacacc tacggtatta gtgcctttgg
       61 ctacaactcc atttaattca ttgtcaaaag tgtagtataa ataatatcca tttcgccata
      121 ctatttcagc agtttttata ttctcaacat taaaatctaa ttctaaatac ctgccttttg
      181 acattgataa ttttaagttg cctttagcat ttctattaat agcactagct ttaaaaggta
      241 ttatataaaa tttcttagtc ttgtatggat attttgcttt agtattacct ttttttctta
      301 attctgatat agtctttcta tttgcatcaa atttatctga tatagcttga atagtttgac
      361 tatgaagatt gtacttacct ttgattaact tttgtatatc attcttttta atccattgtt
      421 tatgttcaaa atagtattct ttagataagt taactatgta gttccaacag ttagcacttt
      481 ccatttgctt ttcccttact aattcaaatt gtgttctagt taggtttaat tttactctat
      541 gagtttttat catttctttg tcctaacttg ttgattttct atatattgtt taactacttc
      601 taaactcaca gaaccaacag tacctacaaa atagctatta gtccacatgc taggcatact
      661 agactttaaa tgtggaaagt tctctcttaa tactctcgaa ctaaagcctt ttaaaccttt
      721 tactacttta tgaattccat attgagggtc acaagatatt aataagtgca catgttcttt
      781 atcagtttcc atttctatta tttctgctcc aagttcatct gctttatatg gtagtatttc
      841 ttttaacatc ttttcaactt cgccaactaa aacaggttta cgatacttag ggcaaaatac
      901 aatatgatat ttgcaatcat aaactatatt tttgttagat ttatacataa aaactaacac
      961 ctcctaaata tagggtatca gtagcggtat tagattacaa gcattattct aacttttttt
     1021 atattaagac cttacctttc atccctacac ctaaaggaga gggcttttcg gcaattttat
     1081 tgtaattata tatcatttat ataaacaagt acatatttta ttttgaaact acaaaattat
     1141 tgtactatat tgttttaagt tgttttataa tagattatat catcttatta taggagaaaa
     1201 aatatgattt gtactattaa accattaaag atttcaataa aaaagtctac tgaacctcta
     1261 attgaattta tatctgatac tacctataaa aataagagtt cttcatatat taaagaagtc
     1321 tactttacaa gatatatagg tgttgatgat tcaattgata aatacataga aaaaattaaa
     1381 aatatagata cttatttttt taataatcct acaattccct atattagatt aaacaatcta
     1441 aaaataaatt ttgacaaaga acaaacagat aagatgctta aaatttttaa tatttataat
     1501 agttctaagt ttaacagttg ggatttatat aatatcgact ttccatgtaa aatagaaaat
     1561 gacaccttaa attggactaa aaagattgca tttaaaaata ctttagattt attttccaca
     1621 agtatgccaa attgtacatc ctctatcatc aaaaactttg cagttaaact tttgtgttgg
     1681 atagactatt ttctacctaa attattttat aacaaggtga agactactat ttcaccaaag
     1741 atagtatact ttggaaatat taaaaggcaa gaactttttt tcttatattt tttatcacaa
     1801 cttgggtgtg atattttata tataaatcca aataaagata ttcttgattt atatcctgaa
     1861 tgttcaaaat tttctactct atttgaactt tctaggaaga ctccaaatat attagaaata
     1921 ccttttgaaa ggcatattac aaatataaat tacagtgaaa ataccaataa gacttctcct
     1981 actacgaata aacctaactc taaaaataca cttaataaca caaatctaac atctaaaaaa
     2041 attgtacaag atgaggaagt agaactttct tatgaagacc ttgcaaagtt atcagcttct
     2101 gttgttatga ttgttgtgtg caataacgaa aataaaccgt ttaagagcgg ttctggagta
     2161 gtaataaata atgaaggtta tatacttacg aatcttcatg tagtcaatga tgggtattca
     2221 tttttagtta gatttgaaaa tgatgataaa gtttatacat cttaccaaat tataaaatac
     2281 cattcagatt atgatttagc tgtgataaag gttgatagaa aatgcaagcc aatacctgtt
     2341 aaagtcagta aaaaacctgt gagaggtcaa aaaatagttg caattggaag ccctctaggt
     2401 ctttttaata cagtttcaga tggaattatt tcagcttttc gtgattttga aacggttcaa
     2461 atgatacaat ttacagctcc aatctctagt ggtagttcag gaggagctct tttagatatg
     2521 tttggaaact tactaggatt gatttccgct ggatatgatg atggacaaaa cttaaattta
     2581 gctgttgaaa gtagtcttgt caaaatattt gccaataatt ttattgagat agtaaactaa
     2641 tcaatcaaaa ttctacttat cctattccat aataatcaag agggtgaaaa cattgcatgc
     2701 ttttgccctc tgattaatat tgaaaaaagt attaatactc gacattttta tgctcaataa
     2761 ataattgatg ccatagacaa aatgtaaata tagtccatat ttttctcgaa ttatctctat
     2821 gacctttaag atgcgtatct aaaagtttta ttgcatattt tttatctata aattcatcta
     2881 catttgaatt ataaatagtt tcctttacaa tatcccctag agaatctttt aaccaaactc
     2941 ttattggagt tggaaatcct aattttttcc tttgaacaac atgttctggg actatttctc
     3001 taaacgcctc tctaagtaaa acttttgtat tattcttatt tattttttgc tccattctta
     3061 aattttttgc gacatcaaat acatctttat ataaaaatgg tactctaagt tcaacctgct
     3121 cagctgtaga aagtttgcta attttttgaa gtatatcgcc ttgaagccat gtattcacat
     3181 ctatatgttg cattttactt atataatcgt aattattttt ttgtgcatct ctatacaaat
     3241 tagacaacag gtattcatat atattctttt ccttatactt aaaaaagaat ctttttactt
     3301 cgctgttttc aaaaactttt gcatttccta tatatctttt ttctaatggt gttgtagctc
     3361 tataaagata atttcttcct tttatgtttg gcataagttc tgaaactctt cctaatatac
     3421 ttttaatata agtaggtgaa ttgacaactg atttcatagt actatactct ttatatatat
     3481 tatatcctcc aaataattca tctgctcctt ctccagacaa tgctacttta acatgtttac
     3541 ctgcctcttt aattaagaaa taaagaccta cttctgaagg gtctgctata gggtcatcca
     3601 aagaataaat tacattcggt aatgatttta tatattcatc ttgtgttata ttaatctgga
     3661 tattttctat acctaactta tcagctgttt tctttgctac ttctaattca ctatatcctt
     3721 tgacactaaa gcctgcagaa aaagatttta tatttggatt aatttgtgaa gccacagtag
     3781 ttataataga tgaatctatt ccaccagaca aaaatgttcc aatttctttc tcctcttcca
     3841 tatgagttac tattgaatct actactatat ttcttatatc atcctcattc acatctctat
     3901 tgcctaagtg atttttagta taaaaaaatt ttgtactact tctaaactca agtttattat
     3961 atctctttaa agatagtata ccattttcta ccctaaaata gtgacctgct ggtatcagcc
     4021 ttattccttg tatcatagta tcttcaggaa gtacatattg aaatgaaaaa taactttgta
     4081 agcttctttc atttatattt aaatttttca tatattcaag taaaaactta tactctgagg
     4141 aaaaaattat tgcattttcc ttatttatat aataaagtgg ttttatccca aataaatctc
     4201 ttgctccaaa tatactttca ttttctctat catagattat aaagttgaac attcctttaa
     4261 tcttatgtac acaattctta ccaaattcta tataagatgc taataagact tcaatatcat
     4321 catttgtagt aaaattgtgt ccttttttaa ttaattcttc ttttatttca tgatagttat
     4381 atatatttcc attaaaaaca ataacttttc catctttttc aaatggttta tttccataaa
     4441 tagcatcaat tacattaaat ctttcaaaat caaaagtttt ttcctcatcc ttatactcta
     4501 ttctactaga aagtcttgta ttatttacag attcttttat tttaaattta atatcttctt
     4561 ttttagatat atcaccttta taataaattg aaatattgcc gtccatataa tcaaaattcc
     4621 tttctgtcct ttagtcttat tctatatttc atagttttgt ctttggcttt aaaacttatt
     4681 caatttaaaa tgaaattgct attaaattct tataaattat ctgttaatat ttcatatttt
     4741 ttttatttct agaaatacta tattaggagg tgatttttat gaagaaaaaa atattaatac
     4801 cagttattat gtctttattt ataatctcac agtgcataac ttcatttgct tttacacctg
     4861 aaaataacaa atttaaggtt aaaccattac cttatgcata tgatgcactt gaaccttata
     4921 tagataaaga aacaatgaaa ctgcatcatg ataagcatta tcaagcttat gttgataaat
     4981 taaatgctgc tcttgaaaaa tatcctgagc tttataatta ttctttatgt gaattattgc
     5041 aaaatttaga ttctttacct aaagatattg ctacaactgt aagaaataat gcaggtggag
     5101 cttataatca taaattcttt tttgatataa tgacaccaga aaaaaccata ccttctgaat
     5161 ctttaaaaga agctattgat agagactttg gttcttttga aaaatttaag caagagttcc
     5221 aaaaatctgc tttagatgtc tttggttctg gttgggcttg gcttatagct actaaagatg
     5281 gcaaattatc tattatgact actccaaatc aggatagccc tgtaagtaaa aacctaactc
     5341 ctataatagg acttgatgtt tgggagcatg cttactattt aaaatatcaa aatagaagaa
     5401 atgaatacat tgacaactgg tttaatgtag taaattggaa tggtgcttta gaaaattaca
     5461 aaaatttaaa atctcaagat taattaaata ttaaaatcct tttgaaaaaa acttatgttt
     5521 gctatataat ataataaaag atagtctaaa tttaccaata taatgtttat agtgacgatt
     5581 caggaggatt tttatatgat aaaagaaaca catgaaaggg gaggttatat attcgcactt
     5641 ttgatactcc cctttataaa caatctgtac ctagttaaat acgatattgc atataaaata
     5701 gtattaatta taatttatat ttattttgca tatttaggtt ctttatttcc agatatagat
     5761 atgagaggtt cttacataag taaaaaattt atgttaatat acaaattatt tggaagtagg
     5821 tttagacata gagggtttac tcacagtcta attgccttat tacttattta tagttttttt
     5881 aaatctctga caatcttcac taacaataat atagttttct catgtttgtc cagtgggttt
     5941 ataataggat atctatctca cttgtgtctt gaccttatta ctaaagaagg tattgaattg
     6001 ttttatccca ttaccataaa catttcttta ctacctataa aaactaactc aaaaacagaa
     6061 aaattcatat ctaagctatt aaatttcata gttatttttt tgattggata tcaattttat
     6121 attttattat agttgtagaa aaaataaata acaatatttt gaactaagta taaataagtt
     6181 ttatatattt tagtttacaa aagaactaaa aattttgtac cttaatacaa aaaataaaac
     6241 gtatcttaaa ataatttagt actaaatcat tttaagatac gttttatttc gtacaagcta
     6301 aatcatttta tcatctttat cattgtgatg ttttttatga tggaaatcat aattatgatt
     6361 ttcaagattt ttatttactc tatttccaat cttccatgac aaaaacacca ataatataaa
     6421 tgatgctatc tttaaaggac ttgttataaa accttctata tcacttgcca cgtaacttat
     6481 aactataaac attacaaatt ttcctgccaa cattgctggc acaaaatctt ttaaatcttt
     6541 tttaccaaaa gctgatgcga tagtaactaa acaaccaggc ataaatggac aggcatacgc
     6601 tataaataat aacttaaatc cctgtttatc catccaactt attgcttttt cagttttact
     6661 atttctcaac ttattaaaaa gcttactatc attaaatctg cttattaata agaataatga
     6721 agttgtccca agtcctgagc caatccatga taacaaacat ccaataaata atccttgtat
     6781 tgcagcattt gctgtaacta tagcaacaag tggtaaggct ggtataaagc tttctataaa
     6841 tgcacttaat aagccgatta ttgtggctaa cacccaataa ttttgagtca aatcaagtag
     6901 ttcttgtata tactgcatat tgcttttccc cttttttaac tttaactatt attatatact
     6961 ctatatgttt attatatacc aatctcaaaa aatgaatcta atattaaaaa gtaaataatc
     7021 tatataagtt tacagttaag gtataattat tttattatat attataacaa aaaaggtgat
     7081 gtttcaaaat aaaaatctga aacatcacct ttttataact taaattgtca tgtaagttat
     7141 aaaaaatata tattagacat aatattacta aaaatgtcta gtcagagtaa aataaattac
     7201 acagtataac aaatattact atttaattga gttacttctt tataacattt aaccttcttc
     7261 ctactctgct caataattca tttgatatac tacatgagtt atttgcaact tcagaagcag
     7321 ataattcagc taaattatct cttcctatta ttattgccgt attaccaact tctacattag
     7381 gtatatctgt aatatcaaca gcaagttggt ccatgcaaat tcgacccact attgctgctc
     7441 tacaatcatt tattattacg taactttttc cacaggataa atttctaggt ataccatcgg
     7501 catagccaat agataatata gcaattacgc tatctctgct tgcaataaag actcttccat
     7561 atccaaaact atcatctttt tgtatttttc taataagaat tacttgtgat tttaaggata
     7621 aaacaggtcg taaatcaagt tgcaatttag tcgtatcatt tggagaactt aatatcccgt
     7681 ataatgcaat tccaattctt acataattac acttaagatt gggataatta aaaaaaccat
     7741 aactactttg aatatgtact ttaggaattt ttataccttt ttttaacaga aaatcaatta
     7801 acttataaaa acattcaatt tgtttatttg taaagtctac atcttcatct ttcagactat
     7861 ctgaaacaca caagtgtgta aatatgccac atatatctaa gtattttaat ttaaatgcct
     7921 ttaaaattga cctcacatca ttaacatcaa aacccagtct gtgcatacct gtatcaattt
     7981 taatgtgtga ttttatgttg tatttctgtt tgtttaaaga gatagcatga ttaaggtcaa
     8041 taacactctg tgtaagattg tatttatgaa gttcctttgc ccttattggg ttagtatatc
     8101 caagaatcaa tatttctcct agtattccat atcgacgtaa atcaatcccc tcatctattg
     8161 ttgctactgc aaatgctttt acacctattt ggtttgcaca agtcgctact tcaaaagcac
     8221 tatgaccata ggcattagct tttacaactg ccatcaattc acaatcttct ggcatagctt
     8281 tttgtatcat atttacatta tgttccaaat tatttaaatc aatttcaatc caagctctat
     8341 tggtatttac cttatgttct cctttcaagt ctttttttag atgtttaaga atcaatataa
     8401 taattataga agctataaaa gatgctatac ttaccactat ataatgaata aggctattgt
     8461 caaccaagac agtttgtatt ccgaatattt tagaaaacat acgaataaca acaatcatca
     8521 taggatgtat aatataaata attaatgatg atgttcgcaa taacctagct cgagaacctt
     8581 tcaaaaatgt taaggctgta aataagaaat acatacatgg caataacata atatacatgc
     8641 tatcatgacg ctgtacccct agtttatgta aaaacattcc ctcacataac ataagtaaga
     8701 atgatataag aaatccaatc aagctatttt tagttgacat ataattagat ttattagata
     8761 ttattccacc aagtatgaaa aatacaggtg caaaaaatat accatttcgt gtataatctg
     8821 atacttcaaa aatataccca tataaattct ttaatagtgg tattttttct aaaaaaccat
     8881 aataactatc accaaacata ccaattacat aaataacaat agtgattact agtgagtact
     8941 ttaaacctag tttttttaca agaaagtatg caatacttgc accaataatt gatgcgggta
     9001 gataccataa atgatataaa gtcccatcaa atacaatgtc cttaataata ttgggcacta
     9061 aattattcat ggaaaaatat ccagtgtaaa tatttaaggg aatataaatt acaatagcta
     9121 tcccgtaaat aaataataca ttttttagaa aagatttcag tttattacaa ttataattat
     9181 atgttgaaat tagaaaaaag cctgatagca taaaaaagaa tggcacagca atacgagcaa
     9241 ttatactagt taacacaaag tcagccattt tactatatga taataatgga gatgtatgaa
     9301 ttgcaattat aagtaatgca gctataatcc taaaataatc aagccctgca taagatttat
     9361 tcctaatcat tcttgttcct ccatttctta gtcctttcta tttctccaaa atgtcacaat
     9421 aaaaccatcc ttattgttac ccgatatttg atatacacat tgttcaggta tagagattct
     9481 cacttcttct ttagtatgga taggcacata aaagatttct ataatttttt ctttatcttc
     9541 tatttttaaa caatttgtat acgaaaatct cttaatatat tctatatatt cttcaagagc
     9601 gagattcatt ttattgataa tctcagaatg cggatatccc acataacgaa aatgccatgg
     9661 ttcttgagat attcctgtta tttcttcttt tttacactga taacgctcta taaaaccata
     9721 atgtagtgct gccttacgaa actcattaca aataccatta taaggaaatt cagggcagat
     9781 aaaatctata acttctttct ttaaccctaa atcaatagca agccctgtct gatgttcact
     9841 gtgatttgga agtgctacaa actttttagt aaagtctttt ccattgtcac gcagagaact
     9901 tgaatatata tcttgttgtt cctctgcagt acgatatcca cttacaggta ctatttcatt
     9961 tattccacat atacttttta aaatattttg taaaatgttt gtagcttcac gtttcataag
    10021 aatacttgga aaccttgtat ctacaggaat taaatctata tcttctgatg ttttaactgg
    10081 taaaaaagca tttactagaa ttagattccc atcataaact ttatctttag tcaataagat
    10141 agtatttgtc attttatctt ctttattttt atctaataaa atattattca tcattttatg
    10201 tacacctcaa ttatcttgtc caatatttct tcaaaagaca gtccaatacc tttcatcata
    10261 tttggataac gactatgaga agtaaagcca ggaattgtat tgacttcatt aaaaataatt
    10321 tcaccagatg gagttaaaaa catatctact ctagcaaatc cagaacatcc aagagttcta
    10381 taaataattt ttgctgtctc ttgtattctt ttctcacaat ccaaatcaat tctagctggc
    10441 atatgaattt ttgaactttt caatgtatat ttttctgtat aatcaaaaaa tccttttgac
    10501 aattctattt catctactct tccaacaagt aagtcatcgt ttcctaaaac tgcacaccct
    10561 acctcaaaac catcaatatt ttcctcaatt attacttcat tatcatgttc aaaagccaag
    10621 tctactgcta cttgtaactc ttctttttta taaattttac taataccaaa tgatgaacct
    10681 gcacgcacag gttttataaa taatggataa gttaagtctt ttgacagttc agccacatta
    10741 aatttatctg agtattttag taatattgat tttggtactt taataccttc tgaatgaaca
    10801 agtttatgtg atttgtcttt gtccatacat aatgctgaag aaagagtacc acatccaata
    10861 acctgtatac ctgccaattc aaataacccc tgcactgttc catcttctcc attttttcca
    10921 tgcagtacag gaaacacaaa atcaagttta gtgtatttaa tatcattttc acatgattca
    10981 atagcactat ttggaatatg attctgagaa acagcatcag gaattaaaaa ttcaataata
    11041 ccatttttga cacggttctg cgaaacaata actggaatta tatcagtatc cctttcaaac
    11101 caagtgttat tctggatatt attataatct ccaatataat gataccaatt accctccctt
    11161 gtaattccaa ttggtataat atcatatttt tccgtattca aattctcaag tactgcaaaa
    11221 gctgattgta atgatacatc atattctgtt gagtttccac caaatataac tgcaattttt
    11281 ttattattca tctttgattc tcctttgttg atttatttat ttttatacaa tctacagaaa
    11341 catgttaaaa tactttttct atttcaaatc caatcaacct tcacttacaa ttgcatctgt
    11401 tccacataat atattttcac atttttctat tgatataatt actgtatcaa aataaaagca
    11461 ctcttgtttt gatacagtaa ttatatcaat aacaagagtg ctctttattt atttatccta
    11521 aagaatttcc tattttttta tacttaaatt cttacaaatt tcctacaatc ggaataataa
    11581 ccttaaaggt aattatattg ttctcgctat gagctgttat tgtcccgtta tgaagctcca
    11641 ctatttcttt agctatagca agtcccaacc cagctctacc actgtttgaa ttacgagatg
    11701 tatccaacct gtaaaattgt tcaaaaatac gctcaagttt ttcctctgga atcgtattgc
    11761 tatgattcat aaatttaagc tttatataat catcctcctg tacagctgtt atttcaatag
    11821 tagtatcttc aaaactataa taaacagcat ttcgtaataa gttatcaaat acacgttgca
    11881 ttttattaac atcacacctt atcatcatat ttggtgtaac attcagtgta tattttaaat
    11941 tcttactcat caaagtaggt ttaaactcat atgttagttg ttcaagcata cgagttaagc
    12001 ttactgtact gtattcaagt gatatatttg ataaattaaa ccttgtaatt tcaaagaatt
    12061 cattaattaa atcttcaaga cgctctgcct tatccaacga aatagataaa tatttttttc
    12121 gcagttcttc agaaatctga ctttcatctc gaagtagtgt cagataccct ataactgatg
    12181 taagtggtgt ttttaaatca tgtgcaagat aaacaataag ttcattctta cgttgttctg
    12241 ccatttgtgt ctcaaattta cgttgttcaa gtatatgttt aattgagttt attttctttt
    12301 ctattgccaa taactctgga gataaagcca cttctccaac atcttctttg ataagagagt
    12361 caataccttg atttatttca ctaaaatatt ttgtaaacca atttaaataa acacggaata
    12421 ttataaaaaa cacaatcacc atagctagta tcatgaagag ctccatgcta ttacggaaaa
    12481 tctgctgata taaattcaat gcagctttat attccatacg aaaagcattt tcaaagaatg
    12541 atactatcca gatagaaaac ttaccataca atataaaaga atataaaatc catatgaaaa
    12601 taatggctat aaatgccatt aaagctgttc tcataaaaat tttatttctc acttgagtaa
    12661 agtcaggatt gactttaata tttttacttt tcaattgtat aacctactcc ccatacagtt
    12721 tttataaatt tcgggttttt agaaggttca tgaatttttt cacgtaatcg ccctatatga
    12781 gccataactg tgttattatt atcaagatat ttttctcccc atacagcttc aaacaattct
    12841 tcagaaggaa ccacttttcc ttgatgttca cataaatacc aaagaataga aaattcaatc
    12901 ggtgttaatg ctacttcttt cccaaataaa ctacatttat gtgtttcttt attgataatc
    12961 aatcctttaa tatcatactc attaacaatt atcgactgtt gttcataaga attattatat
    13021 cgcatatatc ttcttaattg tgtctttact cttgcaacaa cttccaaagg gttaaatggc
    13081 tttgttatat aatcatcagc acctatggtt aaacccatta tcttgtctat atcttcaatt
    13141 ttagctgtta acataatgat aggataatag tatttttctc gaattttctg gcaaatatga
    13201 aatccatcaa tatcaggaag cataatatct aaaatagcca tatccaaatt tttagattca
    13261 atacattcta gagcttcctt acctgtataa aacttgtata ctgtaaaacc ttcatttttt
    13321 aaatatactt caattaaatc tgctatttct ttttcatcat ccacaatcaa aatattttcg
    13381 ttcatagatt atttttcact ctccttatta tcgaggtcat catttataat attggtaaat
    13441 ttacatatca aataattaca agcatatttg taactaattt ttataagaaa agaactacta
    13501 catcagaatg tgttgatgct actattggaa cacaaaaact gagattaatt gttattataa
    13561 aaactcccct aacacgtcct tattatcgca taacaggtat taactttcaa taaaaatatc
    13621 tatcagtcaa acacaattca atttattata ccagagacaa tccatataaa aaacaattaa
    13681 acctatgtat gtaaccacag acattactaa aatttccatg ttattttata taaaaattca
    13741 aaaaaggagt tgaatttaaa atttgattag aaaaaaatca attttaagac aactcctttt
    13801 tattttaatg tatttaaatt atagtatatc agccttcata atatcctcaa aatcaccttt
    13861 ttcagatatt aatcttatca atttaacagt acagcttaaa tcatttataa gtatagctcc
    13921 aactaatgaa ccatctttca taaataattt tttatatatt ctttgacctt catcttttga
    13981 tgaaatttct ttatcaaaat tttgtatatt accaatacta aatactttta cattcattgc
    14041 atctaatgaa actggatata cttcactttc aaatgtcaaa ttatctccac ttgcatttgc
    14101 ccctgctatt tttcccattt ctactgaaga tggccatatt gctaaactaa tatttcccac
    14161 ttgtgcaaca tctccacaag catatatatc tttaacattt gtctgtaatg tcttatctac
    14221 aacaatacct ctatcaattt ttacagatgt atcctgaacc atttgagtgt ttgctcttac
    14281 accaattgaa aatactacca tatcacagtc gacactatct ccatctttaa atacaacttt
    14341 ttgagctttt ccatcacctt caataccatc aactgctaca cccaatctta catctattcc
    14401 tgtatctctt acacattgtt ctaatatttt actaccttct ttatcaagtt gttttgatag
    14461 tatgctatcc atggcttcaa ctactactac ttctaatcct tttagtctaa attcccatgc
    14521 agcttcaagt cccaataaac cgccaccaat cactacaact ttcttagatt tttctagtgc
    14581 ttcttttaca ctatataaat cttttatatt tctaagagta aacacatttt ctaaatcatg
    14641 gcccttaatt ggtggtataa agtttctact tcctgtagct attacaagct tatcaaattt
    14701 aatagctcca tcattcacta taatagtttt attcacttca tctaatttat ctacattcgt
    14761 tcctaatatt actactatat tatttttatc ataccaatct ttatcttcca tgtagaaatc
    14821 tgaaccaagt tcttcattta taccatcaga aagtgcaggt ctatagtaag tcaactcatc
    14881 ttcattagaa atcatagtta tcttacaagt tttatttctt tttcttatag catctgctgc
    14941 ataaaaacca gctgctccat tacctactat tacgaaatat tcatcagtat cttttctaaa
    15001 agtaacttca tcttcttcaa cttctataaa ttggtcttcc ttagctccac atacagggca
    15061 tacactagga gcactatcac cttcaaaaac ttcattacaa attacacatt taaatttctt
    15121 agttttcttt ttacttccaa aagtagaatt taacttatct aggaatttac ctgtataaga
    15181 atgtaattta tcaatctctt catttgatgg tttaaagtta acagcaaaag gttttactgc
    15241 tgtcattcta agctgactta ttctctccat agcattttct ataccttctc cactccatcc
    15301 atatgaacca aatactgaag caacttttcc accatgaaga actgggttta acgatattaa
    15361 tatatcccat ataggcttta acgcatctcc taatattgtt ggtgttccaa ataatacacc
    15421 ttgtgatact gctatttttg ccaaaatatc ttcttgctta tgctctatta catcaaataa
    15481 atttacttta tagttgctat ttttttcaat atatgctttt atagcttctg ccataatctt
    15541 tgtatatcca tgtgcagaaa cataacaaat tgtaacttct ttttctaact ttatttgttc
    15601 atttacactc cagttgtaat aaaggtctat aatttttcta ggattttcag ttaatactgg
    15661 accatgacct ggacatacag tatctatatc taaatctttt atcttttcta tagcagttac
    15721 catagatggt ttaaatggac ccattataca atcataatag tatcttaaag catccaagta
    15781 atccttttct tcattttcat caagtgtatt tactatctta tcattactat agtgactacc
    15841 aaatgaatca catgttacaa gagttttatc ctcttttata tatgtgtata tggtatctgg
    15901 ccaatgaagc ataggtacag agaaaaattc taaagttttg tccccaattg ataatgtatc
    15961 tccatctgta actgcaacat attcaaagtc tttgtttacg atttccttaa gatatttaat
    16021 tgctgtttct gaagctacta cttttgcatt tttagctaaa tccaaaagct tttcaacact
    16081 tcctgcatgg tctggttcag tatgacttac aacaatatag tctatttttt caaaatctat
    16141 atttaaatca tttaatcttt caatataatt gtcaaaatgc ttatctttaa ctgtctcaaa
    16201 taaaactgtt ttttctgttc cttttaatac atatgaatta taagtagtcc cataaggtgt
    16261 atacataatt atgtcaaata ctcttaaatc agggtcaagt gaacctaccc aatataaatt
    16321 ttcttttatt tttagtgatt tcaatgacat accgtactcc tcctaaaata gttatttagt
    16381 ctctttatac cctgcttgtt atattttaaa caaaatttat tcaaaaaaga aattatttta
    16441 aagatagttt aaatcttcta atcttctttt tctaccttca atacagttcc ctttttagaa
    16501 tctactttta tgtcatattc atttttatct ttatctattc ttattttata aaaagtaaca
    16561 tctaaatctt tgtctaaact ccattcttta actattccat tttgttcttt taatgcaatt
    16621 tccatagctt gttgaggtgt cattatgtca tttaaatcta atttttcttt tctagcaact
    16681 ccatttaaat cttctgaatc caacttttct gttttatctt ctaatacatc ttttgtatta
    16741 gcatcaattt tcatcttata ttcattatta tcatctacac cttccacagt ataaacaaat
    16801 ttatttaaat ctctctcaag acttaaatct actatatcag catccttata tttatcttta
    16861 aatatgttta ttgcatctgt gtatggcact ttaaaatctt gttccattaa attttcatta
    16921 tttttttcat cttttaaagc gttatttgat gcatttgcct ccttttttgt attatcttgt
    16981 gcttgtcctg aatccgtagt tggactccca ctacatgcag ttaataaacc tgctataagt
    17041 atacttacca ctaataaata cgttttttta cacataaaaa tcctccttaa atatattaat
    17101 ttattctata ttatgccctc tgaacccttt atttaccctt ttatattaaa aatttctcct
    17161 attttatcaa ttttaaaact ttatatttac taaaattaaa gttacataaa aattatactg
    17221 tctcaaaata gaaacaagca tctttttcat tgttgaaata ataaaaatat attttatttc
    17281 gtacaaaaag agtgcctcat gaactaaaaa gttcattttg agacacgccc attttttata
    17341 tttttactta caaaataaca gtacttatta attttttaca aaccttataa gtatatctat
    17401 atgatattct taaatttaaa cactatatta aaaaattaaa ttttgtttaa ttcttttact
    17461 gttaaatcca ttttaggata tggaatcgta attccttctt cgtcaaacct aactttaacc
    17521 tcttctagta aatcaagata aattgtccaa taatcttctg tcttgcacca aactctagtt
    17581 gcaaatttaa tagcattatc tccatgttct actactccta taaatggttc tggttcattt
    17641 attattaatt tatgattatt aacaatatct ttaagtactc ttctaacatg tagtatatca
    17701 tcttcatacc ctacactaaa agtcaaatca actcgtctag tattctttgc tgaatagttt
    17761 attagactat catttgatac agaaccatta ggtataagta tctgtttatt atctggagta
    17821 accaattgtg tataaaaaag tcctatttgt tcaaccgttc ctccatggcc tgctgcttct
    17881 acgtaatctc ctactttaaa aggtctaatc aatagtataa taaatcctcc agcaaaattt
    17941 gatagactac cttgtaaagc caaacctatt gctacccctg ctgatgctat cacagcagca
    18001 aaactggaca aactcattcc ccaatattcc agaacaatga ttataacggc aactttaagt
    18061 acactcaaaa gaagagactt caaaaacctt cttaaagtta aatcaatatc tcttttacta
    18121 agtactaaca taacatggtt tacaaatttt tttattattt taaatccaat agaaagtata
    18181 agtataccta ttataagttt tactccgctt gtagtagccc attctagcag actttccatt
    18241 atacctccca tatccatctt attaatgtct aaattattta aatctaactt gcttatatca
    18301 tctattttcg tcgtatccat aaaaaactcc ttaccttata ttttagaacc aatattatag
    18361 tttaaagttc ttattttcta tttaatataa tacaatacca tttgtacata gtaaacaaga
    18421 tttatattcc aattatacat cttaggaaaa tgcaaactat ttgttatata tatgcaaaat
    18481 ataatactaa attaaactat taataaaaca ctatatagcc ttaaaatcat cattttttac
    18541 ccctatatga gatgatacta tagttataat acttagtact ataaatacta caaaaccttt
    18601 tttatatcca ttaactatat tatatgaatt tacttgtaaa gagatagcta taatcaaatt
    18661 aataattcca cttcctataa attcaaaagt attaataaca ctagttgcca gtccagaata
    18721 aatttcttta tttttatact tggcatcatt aaaaacctgt agatgtgaca taactataca
    18781 tcccataata aagaatgata ttggtaaaac tataattggt ggttttacct ggtatacaac
    18841 tacaattaca atccatataa acaaatctat cattgcccca aattttatta tattaaattt
    18901 gctagacctt attttagcaa atacaaaatc cataattatt gaaccaaata taaatccata
    18961 tgtaaaaaag gatactataa acgcagaaaa actcttactc acaccataaa cgtccattat
    19021 gtatcttact ccccacaagc ttataaatgc acttgtaaga cccacaaaag agaacattat
    19081 cattgaatta taccatgttg atttgttctt tataacagac ttaataccat caacaatatt
    19141 aaccttctct gaattttcat agggttcagt atctacattg aatccatact cttttggtgt
    19201 atctctaaca ataatataca taaaacatcc aattacaaca ccaattatac cgattaataa
    19261 aaatgaattt ctccaaccaa caagttcaga caaaaacaca agtggaaaag tagcaagaac
    19321 tccacctaaa tttcctacaa atgaaaattt agctgttgca gaagcaaatt ctgacttatt
    19381 aaaccatctc ccttgaattt ttaaaataca aagtaatatt actgatgttc ctgcccctac
    19441 aattacacga gaaatatatg ctaattctat actttgaatc aaaccaaata aaattgaccc
    19501 taaacttgcc attattattc caagactgct aatttttctt gctccatatt tatcaatcaa
    19561 aattcctgca ggtatttgca taaatgcata tgcatatagg caaaaggatg ctatatttga
    19621 aatttgtata gaattaaaac ctaactcatt tgctaaattg tctgaaacaa cagcagttga
    19681 catccttaaa aataatacta aaacaaatga aaatgtaatt ataccccaaa cactccactt
    19741 agcctttgta taacttttca tatcttctcc ctcaaatcca tatattttta aattttcttg
    19801 atataactta tataaagtgt atagttattc ttacaaattt ttacacgcaa tatcattata
    19861 caacatatgt atatatttaa ctagaaataa aacactattc atattatata catgaattaa
    19921 ctacataaat aataagagcc atcttttaac attactcaaa agatggctcc atataaaaac
    19981 atttattcta aatttaattt tttatctaat ataaattttg tagcgaaaaa taacgctatt
    20041 gcaactacaa gactaccaag tattgcaagc ataaaactag gactctgata aagatgatat
    20101 accatatttg tatcttcatt tggtaagatg cttccaactg catctccaac aattgatatt
    20161 actatattta tagctataaa agaagcaaat gccactatgt ttctatgttt attgaatttt
    20221 ggtaattgtc ccattgagat tgatgtatat aataacaaaa taaatgatga atatgaaata
    20281 aacattacta ctactaataa taaaatatct aaaaagtttt cagaaatcca tttaaaacta
    20341 gtgttaaaca aatctaaaat ttcaggtagt aaaattccag aagtaccaaa aagcataaga
    20401 actccaaaag tgaatactgc aactataaaa ctaagtacag cgtatataag tgctgttata
    20461 catttagata atataagtga actagtgcta acaggaagag taaacattaa ataaccttca
    20521 tcccccaaaa gattttttct aaacctttga attgtaacta caatagttaa aactcctaat
    20581 gccataaaaa gacttgttaa tactagtatt cctattccct gtacctgaag tatatttgta
    20641 ttcataaata ttccattaaa tacagctact atcaatatgg ctatatataa cggtataaat
    20701 attcttccac ttgcttttaa ctcatatttt aataatttac ctaacattta aattcctccc
    20761 taaataatgc atctatagat tttccttttt cttctcttat tgcttcaacg tctccttgta
    20821 acactatttc tccttttgat atgaatatta tctcatcaca tatgttttct atttcactta
    20881 ttaagtgggt agcaatcagt aaagtactat cttcagaata attttttaat atagtcttaa
    20941 gtatatatga ccttgcagct gggtcaacac cacctatagg ttcatccaat atatatatgc
    21001 tcgcatttct tgacattact aaaataagtt gaactttttc tttagttcct ttagacattg
    21061 tctttagctt ttcatttaca tcaatatcta agcttttaat catctcattt gctcttctaa
    21121 catcaaaatc actataaaaa tcatagaaaa acttaagtaa atctgatacc ttcatccaat
    21181 catttaaata agttctttct ggtaaatatg aaactatttt tttagtatct atggatggtt
    21241 tcattccatt tatcataatt tcaccattgt caggctgtaa taatccattc attagcttaa
    21301 tcattgtact tttgccactt ccattaggtc ctaacagccc gactattttt ccctttggta
    21361 tatttaaatc aatatttttt aaaacattct tagttccata actcttgttt acacctttaa
    21421 attcaactat tccatcttga gaaacattgc ttatattttt atccatttta taattctcct
    21481 ttcagatttt ctgtaataag ttttagcatt tcatctctat catatccaag cttttctaag
    21541 gttattttta atgtatttat ctccacatta gctatttcct ctttcatagc ttttattttt
    21601 tccttatcat ctgttacaaa tcttccttta gttctctgac tataaactaa accttgtctt
    21661 tcaagttctg ttagagcctt ttgcattgta tttggattaa cttccgcatc ttctgctagt
    21721 gctctcactg tttctaattt agatcctatt ttaatttccc ctgaaattat ttttaacttt
    21781 aaatgttcta ctaattgtat atatataggt ttgttattat caagttccca ttccatgttc
    21841 atgcctcctt attatattat tgtattaagt tgttaataca ataatataat aatacaaagt
    21901 atatgtcaat tactttttag taaaagtata aaaaatttta caaaaaaaat gaacactaca
    21961 ttagagtcca tttttaacac aatttattaa attctttata ttacagatta attaccctct
    22021 tatcaagttg gatacaatta aatgttcaaa ttcttcaatt ggcattggct ttccaaataa
    22081 atatccttgt gccatatcac actttacttc ttttaaaagt ttaaactgtt cttctgtctc
    22141 aactccttct gatatgactt tcatacccaa ctcttttgcc atttctatta ctttagtaac
    22201 aattattttg cttcttttac tattataagt ttccataata aatttttggt caagttttaa
    22261 gatatccata ggaatttctt ttaacatatt taatgatgag taccctgaac caaaatcatc
    22321 catagatatt aaaaatccta ttttttttaa attattcatt atatcaatta aaatatcaaa
    22381 attatcaaat atgatactct ccgttaattc aagttctata tactttgcag gtatattgta
    22441 tttatcaatt atatttttgt atgtttctat aaaattattg caatataaat gtatccttga
    22501 aacattaact gaaacagtaa ctggatttat tcctgcatca atccattttc taaggtatat
    22561 acatacttct tcaagcacat ataaatctaa atttataaca aaaccgtttc tttcaaaaaa
    22621 tgggataaat tcatttggag gtattagccc tttatcagga cgtttccacc taacaagtgc
    22681 ttctgaacct tgtatctctc ctgtattaag ttctatctta ggttgtaagt atataataaa
    22741 ttctttattt tctagacttg aaaacattgt attttccatc tctttttctt ttatctcttg
    22801 gtcatgaatc ttattatcat aaaatgcata tgaactttta tgtcctccct ttgccatttt
    22861 atgaggtata ttagccctat ctatgattga gttgatatct ctatcttctg gaagtataaa
    22921 atagattcca caacttaaaa ctaacttata tttttcttct tgattattat taaacaagtc
    22981 taattcttca aatatagttt ttaacctctt tgttatatct tcttcttcta tatatttcat
    23041 aattataata aaattatccg cagatattct tgcaaacatt tcctcttcaa atatattcct
    23101 cttaataatc tttgttaaat tacgcaaaat ttcattccca acttcatatc caaataaatc
    23161 atttatatat ttaaatttat caatatctat atataataaa gcatacttta cttgagtatt
    23221 tttacatagt attttgttag catcaattac aaatttatct atagtatttg ctcctgtaag
    23281 attatctgta tatgctatcc tttgcaattt aattttaaaa tttaatttat catatattat
    23341 atagaatata agtattacac atattatagc cataataagt attatttgta ttgcatgttt
    23401 gctaataagc tgattattag acacaataga ctcaaagtta tttatggagt tgtttttata
    23461 gagtttgcat aaagaataga ctggtgttgt gtatgtaatt tgtaaaatta tacaaataaa
    23521 taaaaaaaac atcaattttt tttgtaaatt cctcataaaa atttcctccc attaaaacac
    23581 ctctatttta ttagcaaata agtacatctc aaaagtttcc atttcttgta
//
LOCUS       DAGSRM010000053        23572 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.053, whole genome shotgun sequence.
ACCESSION   DAGSRM010000053 DAGSRM010000000
VERSION     DAGSRM010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23572
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.053"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            176..1198
                     /locus_tag="MJE33_002277"
     CDS             176..1198
                     /locus_tag="MJE33_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648209.1"
                     /translation="MVNWEIKKLAKSKSMFISLGILVLILMISIFIAPVLETESYYID
                     KEKGRIEDTRSGIDVGNEKFQNKINVLKQFSNQTDTGEFSKKIELMSKKKLDNLSVNE
                     YKDISFWKVFNYRVTNSLINVGMLIIISIIISNIYVDEVVTSVKDIILSSKEKKKALK
                     SKIFVALLVPLVIYSVYLMGIFIITCMQQGVPVNGDLESFRIVDNVVALKTNLSITKY
                     VILNIGIELLMFEGWAMIAIFGSFISKSSISSISFFIFIISITKIMSVIHILPKKLLS
                     VVSNVNYYDLIFGFNKIIGNYLGDIMIFKVNIDIVNIAIGLLMAILIGAMSLCFLVIK
                     SDYISR"
     gene            1212..2099
                     /locus_tag="MJE33_002278"
     CDS             1212..2099
                     /locus_tag="MJE33_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9648210.1"
                     /translation="MNKLEIENLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQILYNGEDIFKMDADYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLRGKYLNSKIGELLDLVGLYDVRNKSIGKFSGGMKRRVGIAQVLLNDPKIIVLDEP
                     TAGLDPQERARFRNLIAKMSRDKIIILSTHIISDIESVAKETIMIKDGKLLMKGTHKD
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDIDSIVLRIVSEDIPSEDNIQPVQ
                     AKFEDVYMFYFDLEDAREV"
     gene            2105..2878
                     /locus_tag="MJE33_002279"
     CDS             2105..2878
                     /locus_tag="MJE33_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9648211.1"
                     /translation="MMYLVKAEMIKILKSKSVWIIWLFLIVFGFFLIRKFDVLDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKKGKKSVVLSKLVANGLIL
                     SIINISFILLVGIRGFSFFDFKNLNESIHNLWYFGNSNLNLTVIQMYIIVILTTIFAS
                     FIFAQIGLTLSSIFKSAVIPFILGGLIMAIPYFSVGFIPDKAIKFMSVTPNWIMMSQQ
                     MVKYNVPSILIVFSIVISIILMIVLTKITYENFTSSKRF"
     gene            2990..3715
                     /locus_tag="MJE33_002280"
     CDS             2990..3715
                     /locus_tag="MJE33_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY9648212.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLNNIYTAKTKKEGLELFK
                     SINPDLIVLDIMLPDGEGYDICKEIRKTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSDAQNESKNLNNNEEKIISFGPYILNESRAELIKDG
                     KSIGLSAKELKILSLFAHNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKIREKLED
                     NPSKPEYILTIKGLGYKLAVKED"
     gene            3719..5122
                     /locus_tag="MJE33_002281"
     CDS             3719..5122
                     /locus_tag="MJE33_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY9648213.1"
                     /translation="MKWKITRNFIFTIVFVAISVVIINIISILYVISTNSFFKVVDSG
                     NNPEEFARSFEKDLYEKDGEFKLSKIGAEKLEKSNSWIQVLNDLGEEVYGVNVPKDTP
                     KKYTPFQMVNNYKYIETKYVNFVLEKYLNKKHLNIIVGIPSRDISRIILTYSQNNIKK
                     TLNKVIIITLVIDSVVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKDYE
                     FEEDEIQEYTEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKINLVSLVRNIIIDI
                     LNDNRYKNRNIEFESNEDLIEVYVDSILFRRAITNLIFNSIVHNSEGTLISVEIVKKD
                     NIEIIIKDNGIGISKSDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYV
                     SSEIGIGTKIIIEIKQN"
     gene            5133..5957
                     /locus_tag="MJE33_002282"
     CDS             5133..5957
                     /locus_tag="MJE33_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HBY9648214.1"
                     /translation="MDFFKKGISGFTIKILALIFMTFDHIAAFMPQTMQIPIWFHWVG
                     RISAPLFIFMAVEGFYHTSNRKKYISRLYIWSVIMAIGNQIINNVFSHPEGAIIINNI
                     FSTLFLIAIYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIILGIFTVALLFKVTNRVI
                     ALFMILVPVPFLVEGGPIWIILGIIFYLCRGKKFSLSICYVLMCIFIFTTMSNGDYSL
                     KNSILQNYQWMMIASLPLMLLYNGEKGKSMKYLFYLYYPIHVYILYILGIYLINGF"
     gene            complement(6282..6902)
                     /locus_tag="MJE33_002283"
     CDS             complement(6282..6902)
                     /locus_tag="MJE33_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBY9648215.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKETI
                     PGCISCASCFETGKCIRNDKVNELIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
     gene            7168..7500
                     /locus_tag="MJE33_002284"
     CDS             7168..7500
                     /locus_tag="MJE33_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY9648216.1"
                     /translation="MSIDCISNTDLSETGFSYTLSLISGKYKMIILYCLVEFEVVRYN
                     ALKRYINTISYKTLSLSLKELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMC
                     EWGERNRP"
     gene            complement(7620..8261)
                     /gene="pcp"
                     /locus_tag="MJE33_002285"
     CDS             complement(7620..8261)
                     /gene="pcp"
                     /locus_tag="MJE33_002285"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBY9648217.1"
                     /translation="MKILLTGFDPFGGEPINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQEHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTII
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIR
                     GGFIHIPYTTSQVIDKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(8282..9214)
                     /locus_tag="MJE33_002286"
     CDS             complement(8282..9214)
                     /locus_tag="MJE33_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBY9648218.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVVFAKYEEPDENFGKEQADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFDNSSQFAIGVGSIIALISALIITKAKPATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            complement(9236..9901)
                     /locus_tag="MJE33_002287"
     CDS             complement(9236..9901)
                     /locus_tag="MJE33_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBY9648219.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGVATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLKQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSDELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKASTIMAVIALVVATIQFTLYDKKLNKS
                     HKK"
     gene            complement(10473..10949)
                     /locus_tag="MJE33_002288"
     CDS             complement(10473..10949)
                     /locus_tag="MJE33_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HBY9648220.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNVTIVDINNNNNKIELS
                     KFDKIIIGSSIYVGSVSKKIQVLCNDNVELLNKKQVGIFLCCGFSEQADKYLKSNFPS
                     SLLESANAIGIFGSEARLEKMKFLDKLIMKAVSKGNYDSFRISQDNIDNFLINLNS"
     gene            complement(10952..11356)
                     /locus_tag="MJE33_002289"
     CDS             complement(10952..11356)
                     /locus_tag="MJE33_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBY9648221.1"
                     /translation="MKIRNKATNKKKKYVKISIALFFVFICIIIVIVSNLKPENLVVK
                     DIDINNVKNGIYTGSADNNLVKATVSVEVNNGKIQNINILKHDHLLGKPAEKITTSII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            complement(11331..11960)
                     /locus_tag="MJE33_002290"
     CDS             complement(11331..11960)
                     /locus_tag="MJE33_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY9648222.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMTNKLYNTVESNAIDIMNNCSSLPIDKQIIEIMTSFIKT
                     LSSEPEMAKSIMHSRMNIIFASENTNNTPSNNGIKMLDKFLSIGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLIENFLDILVGGLYNENSQQGY"
     regulatory      12326..12369
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            12470..13438
                     /locus_tag="MJE33_002291"
     CDS             12470..13438
                     /locus_tag="MJE33_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBY9648223.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENALKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLYNHEKVSIIALAPLTNIAKALIKDKKAFENLDEFVSMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNIIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQEGIIGCVINDPLAVAYFIDRSICKGFESYVEVVEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDIIDSVEGVI"
     gene            13440..14003
                     /locus_tag="MJE33_002292"
     CDS             13440..14003
                     /locus_tag="MJE33_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBY9648224.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDVYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPL
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFVTQVFTDYGD
                     KLLAVVLVNLGLNAVPKTLKVSLTGGK"
     gene            14011..14700
                     /locus_tag="MJE33_002293"
     CDS             14011..14700
                     /locus_tag="MJE33_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBY9648225.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLKVLKNVTGKYGVLEVINSGSCFELPKDTLEKIKCIIKEKNIKKLFLESHWSYK
                     NRLKEMREYFEIPVVFKIGVETFDNDFRNNILNKNANFKTPQDVKEYFDSPCIMVGIK
                     GQTKEMIDKDIEIILNTFEKATVNVFINNSSSIKRDEELVKWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            14827..15555
                     /locus_tag="MJE33_002294"
     CDS             14827..15555
                     /locus_tag="MJE33_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBY9648226.1"
                     /translation="MMFDRGKWNKLDYNELLEYMMSIQDIKYRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISQGNWKEFLEVAQDTYYEEVRLQGMVIGNINSNFEETLYYVKRFI
                     PKIDNWSVCDGFCSDLKSMKKYKENMYDILKKYVYSKNPWEIRFALVMFLIYYVDDKH
                     IKEIFEYCNNIQSEEYYVKMGMAWLLSICFIKCEEETFLYIKNNNLDDFTYNKMLQKI
                     IESNRVDLEKKNIIRSMKRKTRRC"
     gene            15569..16243
                     /locus_tag="MJE33_002295"
     CDS             15569..16243
                     /locus_tag="MJE33_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBY9648227.1"
                     /translation="MNNNEDRIDKFLELQSKGFKFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYILKDELNYNKSLDNRNLEEEKAKEYEKSKKNTISNRKKVSTKKKISNEKAL
                     SKSVEQIEFNDTGLKNAKPKQNIKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNIKP
                     KKVSNKKDVHIDDEHIDELKTTRIRIDKEIWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            16269..17105
                     /locus_tag="MJE33_002296"
     CDS             16269..17105
                     /locus_tag="MJE33_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648228.1"
                     /translation="MNKIVKKSISLMIILTIFIFMLTACEKDEQPDSVDIQTEDKNEI
                     KIDEGNAKVLNIGKSEIINIDEGDKVDTSTKVENNSTFNISNVELIYNEYDENKKIIS
                     SDSKALLDMTLMPGKVAYIECGHKTFVKGVEVYAYEYEAEGKIVYVNLKENTINIRNN
                     NIKLENSSQYEVLSTSELKKVNESNEGITYQVKVKNSSSKDLGNIILKTAEVNENGEY
                     LTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHKVKI
                     DK"
     gene            17121..17792
                     /locus_tag="MJE33_002297"
     CDS             17121..17792
                     /locus_tag="MJE33_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY9648229.1"
                     /translation="MKVLIVEDNKILLESVVEELSKHFETEKCEDGEEALYLINQNIY
                     DLVILDLMLPNINGFDILKKMRINNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FVLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            17782..19014
                     /locus_tag="MJE33_002298"
     CDS             17782..19014
                     /locus_tag="MJE33_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY9648230.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKDPKDMVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDVGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKVNLEEIDLEKLLEEISEDYIDIA
                     EFQEKRFVFNSKLKNKVIITDKNKLRQLILIFIDNAFKYTKLGDEIALELKEDIEDEV
                     TLLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSIDINVT
                     SDVDIGTTFELSIPKKLK"
     gene            19164..19481
                     /gene="trxA"
                     /locus_tag="MJE33_002299"
     CDS             19164..19481
                     /gene="trxA"
                     /locus_tag="MJE33_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBY9648231.1"
                     /translation="MAKVINTSEFRSSVEGSKGVVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            19498..20376
                     /locus_tag="MJE33_002300"
     CDS             19498..20376
                     /locus_tag="MJE33_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBY9648232.1"
                     /translation="MRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLVKAP
                     SIDNYLGFYDISGDELKDKFKSHIDSMDISIENKRINNIYAMGEYFTIMSGNDMYEAT
                     TVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEANFLS
                     ELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKENHIEVDG
                     VFVIKDSTAPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAGQGQ
                     IAALNAVYYLDKLKRA"
     gene            20646..21014
                     /locus_tag="MJE33_002301"
     CDS             20646..21014
                     /locus_tag="MJE33_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBY9648233.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIKKCCKRREK"
     gene            21017..21388
                     /locus_tag="MJE33_002302"
     CDS             21017..21388
                     /locus_tag="MJE33_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HBY9648234.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTAVDAVVVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(21533..21988)
                     /locus_tag="MJE33_002303"
     CDS             complement(21533..21988)
                     /locus_tag="MJE33_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648235.1"
                     /translation="MNINTVQGDSIEVLLRQLGATRISKVSSTLYFIKFDLGDGWEIS
                     YTYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEV
                     TRKVNSIIDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
     gene            complement(22027..23292)
                     /locus_tag="MJE33_002304"
     CDS             complement(22027..23292)
                     /locus_tag="MJE33_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HBY9648236.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSIAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVIVILAITIICFRFIYGKELVVNERAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
BASE COUNT         9108 a         2617 c         3664 g         8183 t
ORIGIN      
        1 aaaaataatt gaaaatgtat aatttcccag aaaaataata aatgtaactc caataataaa
       61 cacacctata aattataaaa taatttaagc tttatttaag gttagtatta tatttatata
      121 agatttaagt tttaatatat atgtgtaatt aaaatgattt aggaggagaa atatattggt
      181 aaattgggaa ataaaaaaat tggctaaatc taagagtatg tttatatctc tagggatatt
      241 agtgttaata ctaatgatta gtatctttat tgcgcctgtt ttagaaacag aaagttatta
      301 tatagataaa gaaaaaggac gtatagaaga tacgagaagt ggtatcgacg taggtaatga
      361 gaaatttcaa aataagatta atgttttaaa gcagttttca aatcaaacag atacaggtga
      421 attttctaaa aaaattgaat taatgtcaaa gaaaaaactt gataatctaa gtgtgaatga
      481 atataaggat attagttttt ggaaagtatt taactataga gttacaaatt cacttataaa
      541 tgtaggaatg ttaatcatta tatctataat tatatccaat atttacgtag atgaagtcgt
      601 tacttcggta aaagatatca tactatcatc aaaagagaag aaaaaagcat taaagtctaa
      661 aatatttgtt gctttattag ttccattagt aatttattct gtttatttaa tgggaatatt
      721 tattataact tgtatgcaac aaggagtacc tgttaatgga gacttagaat cttttagaat
      781 agtagacaat gtagttgcat tgaaaactaa tttaagtata acaaagtatg taatattaaa
      841 tataggcata gagcttttga tgtttgaagg atgggccatg atagccatat ttgggtcttt
      901 tataagtaag tcttcaatat cttcaataag tttttttata tttattatat ctataactaa
      961 gataatgtcg gtaatccata tattacctaa aaaattactt tcagttgtat caaatgtaaa
     1021 ttattatgat ttgatatttg gttttaataa aataatagga aattatctag gtgatattat
     1081 gatatttaaa gtgaatatag acattgtcaa tatagcaata ggacttttga tggcaatatt
     1141 gataggtgct atgtcattat gttttttagt aataaaatca gattatataa gtagataaaa
     1201 ggggagttaa gatgaataaa ttagaaatag aaaatctttc aaagatatat ggtaaaaaag
     1261 tagcaaacga caagattaca gtaactctag aaaatggagt ttatggtctt ctaggaccaa
     1321 atggagcagg aaaatctaca ttaatgaaac aaataacaac gttgataaaa cctgatgaag
     1381 gtcagatact ttataatggt gaggatatat ttaaaatgga tgctgattat agaaatattt
     1441 taggatattt gccacaagaa tttggagttt ataaaaattt tacagcaaaa gaatttttac
     1501 agtatattgg agctttaaaa ggtttaaggg gaaaatattt aaatagtaag ataggagaat
     1561 tattagattt agtaggtctt tatgatgtga gaaataagtc tataggaaaa ttttcaggag
     1621 gtatgaagag gagagttggg atagcacaag ttttactaaa cgaccctaag ataatagtcc
     1681 ttgatgagcc aacagcaggt cttgacccac aagaaagagc tagatttagg aatttaatag
     1741 caaaaatgtc tagagataag ataataatac tttctactca tataatttcc gatatagaat
     1801 cagttgcaaa ggaaacaata atgattaaag atggcaaact cctaatgaaa ggaacacaca
     1861 aagacatatt aagtcacatg gaaaataaag tatataatgt tcaagttaaa aatgaatgtg
     1921 aagttgatga tatacaatct aaatataagg ttgtaagtat tcaaagtgat attgattcaa
     1981 tagtacttag gattgtttca gaggatattc caagcgagga taatatacaa ccagttcaag
     2041 caaaattcga agatgtatac atgttttatt ttgatttaga agatgcaagg gaggtgtagt
     2101 agaaatgatg tatttagtta aagctgaaat gataaaaata ttaaaatcaa agagtgtttg
     2161 gataatttgg ctgtttttaa tagtttttgg attttttctg attagaaagt ttgatgtttt
     2221 agatacttat gcagatatat tttacaagat agaaggctca atacctctaa taggtttgat
     2281 aatgtttata ataacttctg gaaattacat taaagaatat gattctaata tgacaggttt
     2341 aataaataca actaaaaagg gaaaaaaatc agtagtatta tcaaaactag tagctaatgg
     2401 attaatttta tctattatta atatttcttt tatattgtta gtaggaatta gaggttttag
     2461 tttttttgat tttaaaaatt taaatgaatc gattcataac ttatggtact ttggaaatag
     2521 taatttaaat cttacagtaa tacaaatgta tataattgtt atattgacta ctatttttgc
     2581 ttcttttata tttgcacaaa taggcttaac actttcatct atatttaaat cggctgtaat
     2641 tccatttata ctaggtggat tgataatggc aattccatac tttagtgtag gttttatacc
     2701 agataaagct ataaaattta tgtcagtgac tccaaattgg attatgatga gccaacaaat
     2761 ggttaagtat aatgtaccaa gtattttgat agttttttct atagtaatct caattatatt
     2821 aatgatagtg ttaactaaaa taacatatga aaattttaca tcaagcaaaa ggttttaact
     2881 aaaaataata gtaattttat ttatatttta gtttaattaa aaataagata aatgaatttt
     2941 agataaaata tatgctaata tatatgtaaa ttattacagg aggttgttta tggaaaatcg
     3001 tgttttaata atagatgatg aagtagaaat attgaagctt ttagaaactg tcctaaaaaa
     3061 agagggtctt aataatatat atactgcaaa aactaaaaaa gaaggattag aattatttaa
     3121 aagtatcaat cctgatttga ttgtattgga tattatgtta ccagatggcg aaggttatga
     3181 tatttgtaaa gaaataagaa aaacctccaa ttctccaata atatttttat cagctaaaac
     3241 cgaagaatta gataagttac ttggactagc gattggagga gatgattatg ttacaaaacc
     3301 ttttagccct aaggaagtgg cttttagagt taaagcgcat ttgagaagat taagttattt
     3361 tagtgatgct caaaacgaaa gtaaaaatct aaataataat gaagaaaaaa ttatatcatt
     3421 tggcccatat atattaaatg aaagtagggc agaacttata aaagatggaa aatctatagg
     3481 actgtctgca aaggaactaa aaatcctatc tttatttgca cataatcaaa atcaaattat
     3541 aagtaaagaa aaactatggg ataaagtgtg gggagaagat tatgttggat ttgacaatac
     3601 tataatggtt catataagaa aaattaggga aaaattagag gataatcctt caaaacctga
     3661 atatattctg acaataaaag gtcttggata taaattagca gtaaaggaag attagaatat
     3721 gaagtggaag attactagaa attttatatt tacaattgtg tttgtagcta tatcagtagt
     3781 aattataaat atcatatcta tattgtatgt gatttcaact aatagctttt ttaaagttgt
     3841 agattctgga aataatcctg aagaatttgc acgttctttt gaaaaagatt tatatgaaaa
     3901 agatggagaa tttaaactat ctaagattgg agctgaaaaa ttagaaaaat caaattcctg
     3961 gatacaggtt ttaaatgatt taggagagga agtttatgga gtaaatgtgc caaaggatac
     4021 tcccaaaaaa tatacaccct ttcaaatggt gaataattac aagtatatag aaacaaagta
     4081 tgttaatttt gtactagaaa aatatttaaa caaaaagcat ttaaacataa tagtaggtat
     4141 acctagtaga gatatatcaa gaataatttt aacctattct caaaataaca ttaaaaaaac
     4201 tttaaataaa gttatcatta taacactagt aattgatagc gtggtagctt taggcgtagg
     4261 atatttattt agtagaaaac ttacaaaacc aatttcaagc gtactatgga gtattgagac
     4321 tatggctaat ggaaattata gtttatatct taaggacaga ggtatatatg aagaagtttt
     4381 taaaaatata aatatgttag ctgatacttt aagagtaaat gaagttgaga gaaaagaaaa
     4441 tgaagagttg agagaggaat ggctggcaaa tataacacat gatataaaga cacctctagc
     4501 atctatacaa gggtatgcag agataattaa tgacaaagat tatgagtttg aagaagatga
     4561 aatacaagag tatacagaaa ttatatataa taagtctaag tatataaaag atttagttga
     4621 tgatttaaat ttatctactc gattaaaaaa taatactata gttctggaca agaaaaaaat
     4681 aaatttagtc agtttagtca gaaatataat aattgatatt ttaaatgata atagatataa
     4741 aaataggaat attgaatttg aaagcaatga agatttaatt gaagtatatg tagattcaat
     4801 cttattcaga agggctataa ccaatcttat atttaattca atagttcata atagcgaagg
     4861 aactttgata agtgtagaaa ttgtaaaaaa agataatata gaaattataa taaaagataa
     4921 tggaattgga ataagcaaaa gtgatttgaa acatattttt aaaaaatatt atagaggtac
     4981 taatacaggt gagatgcata aaggttcagg tcttggtatg gctatttcta aagaaataat
     5041 agagattcat aaaggtaaaa tttatgtaag tagtgaaatt ggaataggaa caaaaattat
     5101 tatagagata aaacaaaatt aggagatatg atatggattt ttttaaaaaa ggtataagtg
     5161 ggtttactat taagatttta gcattaatat ttatgacatt tgaccatata gcagcattta
     5221 tgccacaaac aatgcaaata ccaatttggt ttcattgggt aggcagaata tcagcaccac
     5281 tgtttatatt catggctgtt gaaggatttt atcacactag taacagaaaa aaatatataa
     5341 gtagattgta tatttggtct gttattatgg ctataggaaa tcaaataatt aataatgtat
     5401 tttcacatcc agaaggagct ataattataa ataacatttt ttcaacatta tttttaatag
     5461 cgatttattt acaagcaata gagtttataa agaaatttag aaaagagaaa gaaattaaat
     5521 attttattat aggattattg atgataatca ttcctataat tttaggaata tttacagtag
     5581 ctttactttt taaagtcact aacagagtta ttgctttatt tatgattttg gtaccagtac
     5641 catttttagt agaaggtgga ccaatttgga ttatacttgg gattattttt tatttgtgta
     5701 gagggaaaaa gttcagtttg agtatttgtt atgttttaat gtgtatattt atatttacaa
     5761 caatgtcaaa tggagattat agtttaaaaa attctatttt acaaaattac caatggatga
     5821 tgattgcttc tctaccttta atgttacttt acaatggaga aaaagggaag agtatgaagt
     5881 atttatttta tttatactat ccaatccatg tatatatatt gtatatctta ggaatatact
     5941 taataaatgg tttttgattg taagtgatat ttttaataca aaatgaacaa tatgaggatt
     6001 gttttaggtc tattaaaatt gttatttaga tgagatttgt agaatattta aatttatgag
     6061 cagaaattca gttattgtaa tttcaataac attagaatat aatattataa tttatttaga
     6121 aatatatgat gttatacatg gtttggttat taatgtaata ataaaacaga ggtttgatat
     6181 aaaattcaat acctctgttt tagtgctttt tatcctacat ttatgcttat tttatttata
     6241 aattagtgtt ttgttttaat taatattagt gcatttttta tttattttat aaaattagtc
     6301 ataattgttt cttcatattg tggttcttta attccagctt ttttacctgc tgatatacac
     6361 ttaagaagcc atgccatatt ttctcctaga gttcgcatag tctgtaatcc ttcttcatcc
     6421 ttcataacat cctctggagt aaagccatgt acctgattcc aatactgtga tgatacaata
     6481 ggcatattgc tgattgaaaa atatttattt agttggtcaa atgttgcact tgcaccacca
     6541 cgtctacatg acacaactga agcacctaat tttgtagcca tgcgacttcc agctatgaaa
     6601 aatagacggt caagaaaaga agttaattga ccagttgcac tagaaaagta aacaggagaa
     6661 ccaataataa taccatcaat attatcaaga tcttcaatca attcattaac tttatcattt
     6721 cgaatacatt ttccagtttc aaaacaggat gcgcaactaa tacatcctgg gattgtttct
     6781 ttacctaaat acagtatttc tgttttaata tcgtgtttac tcaatacttt agtaatctca
     6841 tttagtgctg tataagtaca tccatattgg tttgggctac cgtttatcaa taaaactttc
     6901 attataaatt tcctcccatg aattttattt aatagatttt ctaagagata ttatttttta
     6961 taaaatagga ttttcatgaa tattatcaat ttatatagaa atgtctaatc ttagaatcct
     7021 atttaaaata actttttttt tagtaaatac acctcctatt ataaaattga ctttcaatta
     7081 ttcttacact ataattttat tataaattat tctaaaaaca agtacgcaca tttttgttca
     7141 atactaacca aaaggagagt gtaatatatg agtatagact gtatatcaaa tacagattta
     7201 agtgaaacag gatttagtta tacactttca ctgattagtg gtaaatataa gatgataatt
     7261 ctttactgtc ttgtagaatt tgaagttgta agatataatg cattaaaacg ttatataaat
     7321 actatctcct ataaaacttt gagtttatca ttaaaagaat tagaagcaga taacttgatt
     7381 attcgtactg aatatcctca aatacctcct aaggtagaat atagtctatc agaaagggga
     7441 aaatctctga ttccaatttt agatgcaatg tgtgagtggg gagagaggaa tagaccttaa
     7501 ttttgtatct gtagatacca taaaaagact gtgagtaaaa ttgtttgaat aatctactca
     7561 cagtctatat ttaaattaat agttagtatt tttaatttat aaaaatacta aaaaatttat
     7621 tatgaaattt caccaccaat ttcttttaca tcttctttat atataataca agcttctata
     7681 gctaattcta agccttttac tatttcttct aaagacatga atggtgtatt ttttttatct
     7741 attacttgtg aagttgtata tgggatgtgt ataaatccac cccttatatt aggatatttc
     7801 ttgtctatca tataaagaat tccatacatc acatgattgc aaacaaatgt tcctgcagtg
     7861 tttgaaacac tagctggaat tttattttca ttcatatgtt ttaccatagc ttttacaggc
     7921 aattttgaaa aatatgcagg ttcaccatct tcttttataa tagtatctat aacctggtta
     7981 ccctcattat cttttattct aaaatcatct atatttatag caactctctc aggtgtaatg
     8041 tcaaatctac ctccagcttg tccaactgat attactatat ctggattgtg ctcttgaata
     8101 gctttatcta tagcttcaac agattttgtc ataactgttg gtatagttat ctttatgatt
     8161 tctgcaccat ttatattatt attgaccctt tcaactgctt cttgagctgg gtttattggt
     8221 tctccaccaa atgggtcaaa tcctgttaac aaaattttca ttattttacc tcctaattat
     8281 attaaaatcc taaaaagtac attactaata tgtgggctat aattaatacc aaagcaactg
     8341 gagcttgata ttttataaca atataatcat ttttacattc tagcaaggct gctggaacta
     8401 tattaaaatt agcagccatt ggagtaagta gagttccaca atatcctgct gttaatgcta
     8461 atgctccaat tatagcagga tttccacctt gagacaatac aaaaggaata ccaattcctg
     8521 ccgttatgac tgaaaatgcg gcaaatgcat ttcccattat catggtaaat ataaccatac
     8581 ctaatacata agctataaca ccaaataatc tgctatcagc agggactaca ccagatatca
     8641 tggttgaaat aacttcaccg actccggctt gagtaaataa agctcccaat gcaactaaaa
     8701 gttgtggtaa catactagca ggtcccattt gttgaagtaa tcttgagcca tcttgaactg
     8761 atgtagcagg ttttgcctta gttattataa gggctgatat taatgcaatt attgaaccca
     8821 cacctattgc aaattgagaa gaattatcaa aattagaaag gctcatagct actactactg
     8881 cagcaaatgc taatattaat gatggtataa atattttatt tttaagtttg tctgcttgtt
     8941 cttttccaaa attttcatct ggttcttcat actttgcaaa tacaacttgt ttagaaagtg
     9001 ttaataaact agataatatt aatattattc ctattatatt ggctggaatc aatctaccaa
     9061 atatgaatgg cagtgatata agtatccaaa ataatgatgt accatatttc ttattgttat
     9121 ttatggtagt aaaagactta tatgctataa caatcattaa aaatcccatt agtgcgtaaa
     9181 agatttctaa tgatatgttt ataaaatttt gcatttttat tcctcctaaa taaaactatt
     9241 ttttatgact tttgtttaat tttttatcat ataaggtaaa ctggattgta gccactacaa
     9301 gtgctattac tgccataata gtactagcct ttacaatatt aagtccagtt acattgtatc
     9361 caaattgagt cagagtagat gctataagta atactccact actacctgca aataagtttt
     9421 gaccataaaa atttccatag ttttctgatg cagcagataa tgcctttatg gcttcttcat
     9481 ccgattcttt tagctcatca ctattactta aaccagcagc ttgtgccata ggattaacaa
     9541 gtggtcttac aaattgagga tgtccactca ttcttattga tagagcacca gctatttgtc
     9601 tagctatcat gtatatagta agtacttttc ctgttgtaag tctttttaat ttttctatta
     9661 aagaaacagc tctttgtttt aaaccatatc tttctaatac accgatcact ggcaatgtta
     9721 atatgaataa tgatacacct ctgtttgaga caaaagaatc tcctaaaatt gtaaaaattt
     9781 gattaaaatc taatcctgca acaattcctg ttgccactcc tgctaataat acagtaaata
     9841 gtgtgtcaat tttaagtata aatccaatta caactatcaa tataccgatt agttttatca
     9901 taaattgttc tccttccatt tctatatgtt ttaatttaaa tttatttaat ggataaagtt
     9961 aaactctttt tatagtattt gcgttaatag gactattatt ataaaattta ataaaaaata
    10021 gggaagatgt tttaaagtat caaagtaaat aatggttgtg tataggaatt tgaataacaa
    10081 agattatatt ttaagctaat atgaattgat tttgtaagtc tatagtttaa gtatatgtca
    10141 atatttgaaa tagtagtttt aaattaaaca ttatattata aaaagagaat gtgccaaaca
    10201 tagagacaag tgtatttttc tctgttgaaa tgataaaaat atatttgatt tcatacaaaa
    10261 agagagtgtc taatgaattt tttagttcat tagacactct ctttttgtta tgattaataa
    10321 aacatatttg ttatgattaa taaagcatat attaagtgct agtttcatta gatttcacta
    10381 agaaaaataa aattttgata aagtttaaag actattttga gcaatattac ttataaataa
    10441 aagaatttat catagaaata attagcttta ttttagctat taagatttat taaaaaatta
    10501 tctatattat cctgtgatat tctaaaactg tcataattac cttttgaaac agccttcata
    10561 ataagtttat ctaaaaattt cattttttct aatcgagctt cactaccaaa aattccaata
    10621 gcatttgcac tttctaataa ggatgatgga aaatttgatt ttaaatattt atctgcttgt
    10681 tcagaaaagc cacagcaaag gaaaatgcca acttgttttt tgtttaataa ctctacatta
    10741 tcattacata gcacctgtat tttttttgaa actgaaccaa catatataga acttccaata
    10801 ataattttat caaattttga taactcaatt ttattattat tattattaat atcaacaata
    10861 gtgacattat ctgatagttt attttttagt atatttgcac agtcttttgt acaaccatat
    10921 ttacttgaat atataataat agtattcata attattctcc ctttcttaaa gcattttcta
    10981 cagcttttcg tatagtatta ctgctataag ttgcagaagt tatagcatcc acatctaaag
    11041 actgttgttt aataatactt gttgtaattt tttcagcagg tttacctaaa agatggtcgt
    11101 gttttaagat attaatattt tggattttac cattattaac ttcaacagac acagtagctt
    11161 ttaccaagtt attatcagca cttccagtat aaatgccatt tttaacatta ttaatatcta
    11221 tatcttttac taccaagttt tctggcttta agttgcttac aattacaata ataatgcata
    11281 taaatacaaa gaatagagca atagatattt ttacatattt tttcttttta ttagtagcct
    11341 tgttgcgaat tttcattgta aagtcctccc actaaaatat ctaagaaatt ttctataagt
    11401 tgagaaaaat tatgtaaaga atatagttta ttttcaacaa cacataaaac gaatcctaac
    11461 aatgcactta taagcatcca tgagctttta tctatatttt ttttgaatat tccttgttca
    11521 attccaatgg aaaggaattt atcaagcatt tttataccat tattgctagg cgtattgttt
    11581 gtattttcag atgcaaatat aatattcatt ctactatgca tgatagattt tgccatctct
    11641 ggttcactag aaagagtttt tataaagcta gtcataattt ctatgatttg tttatctatt
    11701 ggtagtgatg agcaattatt cataatgtca atggcattac tttctactgt gttataaagt
    11761 ttattggtca tatcgctgat aatctctgct ttgtctttgt agtaaagata aattgtagtt
    11821 ggagaatatt ctatttttgt agcaattttt cttattgata aattttcata tccatgttgg
    11881 tttataattt cgatgctagc atccataatc tttttcttca tttcttcttt ttcattttta
    11941 cgtctttctt tgatactcat ataatcacct ctttgcttaa cggtgttaag ttaatttaca
    12001 ctgttattgt ataatttaat tttaatactg tcaataaatt attaaataat ataagttaat
    12061 atatttttat ggataataaa ttaatgttat taattgtaat atttaagata ttattgtata
    12121 ataataatcg gtttaaatgc gccacatctt attaaagttg gttcataatt ctaaataaac
    12181 tttaataaga tacattaaaa tataataaat tcttttatgc tataaaattt attttatagc
    12241 tattaaatca gatgtttaaa aattttttaa cggtctatag tttttaagag tcttatagta
    12301 agtagcctaa aaatatgatt ttacatctgt acttgttcga gaactccaag tataaaaaac
    12361 taaggaatta tatagtggca gtatataatt aataccctta gacacacctt tttgaaagtg
    12421 gaaaaaagat gtgtagttct cctcgaccac aaatcaagga ggataaaaaa tggaaaagag
    12481 aaaagtaata attgattgtg acccaggaat tgatgattct ttggcaattc ttctggcttt
    12541 aaactcacca gagctagaag taattggaat taccacatgt tgtggaaatg tcccagcaaa
    12601 tataggtgca gaaaatgcac taaaaacact tcaaatgtgt tcttcactaa atattccagt
    12661 atatatagga gaagaagcac cactaaaaag aaaacttgta acagctcaag atacacatgg
    12721 agaagatggt attggagaaa acttttatca aaaggttgta ggagctaaag caaaaaatgg
    12781 agcagtggat tttataataa atactttata taatcatgaa aaagtatcaa taatagcact
    12841 tgcaccactt acaaatatag ctaaagcact tattaaagat aagaaagcat ttgaaaatct
    12901 cgatgagttt gtatctatgg gaggagcatt taggattcat ggaaattgct ctccagtagc
    12961 agagtttaat tattgggtag acccacatgg agcagattat gtttacaaga atttatctaa
    13021 aaaaatccac atggtaggtt tagatgtaac tagaaaaatt gtacttactc ctaatattat
    13081 tgagtttata aatagacttg ataagaagat ggcaaagtat ataactgaaa taactagatt
    13141 ttatattgat ttccattggg aacaggaggg aataattggc tgtgtgataa atgaccctct
    13201 agcagtagca tactttatag atagaagtat atgtaaagga tttgaatcat atgtagaagt
    13261 tgtagaagat ggaatagcta tgggtcagtc tatagtggat tctttcaatt tctataaaaa
    13321 aaatcctaat gcaattgttc taaatgaagt tgatgagaag aaatttatgt acatgttttt
    13381 aaagaggctt tttaaaggtt atgaagacat tatagactct gtggaaggag tgatatagtg
    13441 tgaaaagaaa aatatgtgta aaaactatta cacttatagc ttttggtatt tctataaata
    13501 taataggcgc ttttatagct atgggattaa gattacctgt atacttagat tcaataggaa
    13561 ctataatgat agcttcactt ttaggaccta aatacgctgt tgttacaggt gtgtttggaa
    13621 gtttaataag tggaattacg tttgatgtgt attctctcta ttttgcacca gttcaaatat
    13681 caaccggatt acttgctgga atagcattta aaaagaattt tttaaggggt ttaaaaactc
    13741 cactaggagt acttttattt gccattccaa catctataat aagttcaata atttcagcat
    13801 ttttatttgg aggtatgacc tcttcaggtt catcatatat agtgcaaatc ttgaaagtgt
    13861 taggactggg ggatgttttt agtgtgtttg taacccaagt atttactgat tatggagata
    13921 agctgttagc agttgtttta gtgaatttag gattaaatgc agttccaaaa actttaaagg
    13981 tatcattgac tggaggaaaa tagatagaaa atggatagat atagtgagat aacaaataaa
    14041 aatcaaagag aaattgttct gcttaaagga tttccttgta tatggggaaa gtgttctttt
    14101 tgtgattata tagatgataa ttctaattta gaagaagaga tgaataaatt aaatctaaag
    14161 gttttaaaaa acgtaactgg gaaatatgga gttcttgaag tcataaattc tggaagctgt
    14221 tttgaattac ctaaggatac tttagaaaaa ataaagtgta ttataaaaga gaagaatata
    14281 aaaaaattat ttttagaaag tcattggtct tacaaaaata ggcttaagga aatgagagag
    14341 tattttgaaa ttcctgtggt atttaaaatt ggagttgaaa cctttgacaa tgattttaga
    14401 aataatattt taaataaaaa tgcaaatttt aagactccac aagatgtaaa agaatatttt
    14461 gattcacctt gtattatggt tggaataaaa ggacaaacaa aagagatgat agataaagat
    14521 atagagatta tcctaaatac ttttgaaaaa gcgacagtga atgtttttat aaataattct
    14581 tctagtataa aaagagatga agagttagtg aaatggtttg ttagtaagta taaattcttg
    14641 gatgaaaatc caaatataga agttttatac aataatacag actttggtgt aggagattag
    14701 cattaattta gtttaaattt aaaattgaaa agtttcttat gtttttgaaa aagcttctaa
    14761 tagttagaag ctttttttgt agagttttat gttacaataa gaaaaaagga attaaggagc
    14821 aattaaatga tgtttgatag gggaaaatgg aataaattgg attataatga acttttagaa
    14881 tatatgatgt ctatacaaga tataaaatat agagatttta ataaaaagct tatacctgga
    14941 acagaaaata taataggtat tagagttcct aatttaagaa agttatcaaa agaaataagc
    15001 caagggaatt ggaaggaatt tttagaagta gcacaggata catactatga agaagttagg
    15061 ctacaaggta tggtaattgg caatataaat agtaattttg aagaaacttt atactatgta
    15121 aaaagattta tacctaaaat cgataattgg tcagtttgcg atggattttg ttctgattta
    15181 aagtctatga aaaaatataa agaaaatatg tatgatatac ttaaaaaata tgtttattca
    15241 aagaatccat gggaaattag atttgcatta gttatgtttt taatatatta tgttgatgat
    15301 aaacatatta aagaaatatt tgaatactgt aataatatac aaagtgaaga atattatgtt
    15361 aaaatgggta tggcatggtt gctatcaatt tgttttatta aatgtgaaga ggaaactttt
    15421 ctctatataa aaaataataa tttagatgat ttcacttata ataaaatgct acaaaaaata
    15481 attgaatcaa atagagtaga tttagaaaag aagaatataa taagaagtat gaaaagaaag
    15541 acaagaagat gttgacggag gaatattaat gaataacaat gaagacagaa ttgacaaatt
    15601 tttagaactt caaagcaaag gatttaaatt tgcagagata gctcaaaatc taaagattag
    15661 tcaaagtact ttaagaactt ttttaaataa aaaaggatat aagtcagaaa aaggtaaata
    15721 catattaaaa gacgaattaa attataataa aagtttggat aatagaaact tggaagaaga
    15781 gaaagctaag gagtatgaaa aaagtaaaaa gaatactata agtaatagaa aaaaagtaag
    15841 caccaaaaaa aagatttcta atgaaaaggc tttaagtaaa agtgttgaac aaattgagtt
    15901 taatgataca ggtttaaaaa atgcaaagcc aaaacaaaat attaagccta agaaagatag
    15961 aaaaataaat ataactcaag aagatttaga taagctttgt gaagtatatg attggtattt
    16021 agaagtaaaa gattacaata taaagcctaa aaaagtatct aataaaaaag atgtacatat
    16081 agatgatgaa cacatagatg aattaaaaac aacaagaata cgtatagata aagaaatatg
    16141 gaatgatttt gaaagattat gtagtaattc gaattttgaa aaacatcaaa tacttacaca
    16201 ggcattgaaa gaatttatga aaaagtataa aaatctacta taaaatacta ctatatgagg
    16261 tgcttaatat gaataaaata gtaaaaaaat caatttctct aatgattata ttaacaatat
    16321 ttatttttat gttgacagca tgtgaaaaag atgaacagcc tgattcagtt gatattcaaa
    16381 cagaagataa aaatgagata aaaatagatg agggaaatgc aaaagtattg aatataggaa
    16441 aatcagaaat tataaatata gatgaaggtg ataaagtaga tacatcaaca aaggtagaaa
    16501 ataattcaac atttaatata tccaatgttg aattgatata taatgaatat gatgagaata
    16561 aaaaaattat atcttcggat agtaaggctt tgttggacat gactttaatg cctgggaaag
    16621 ttgcttacat agaatgtggg cataaaactt ttgtaaaggg tgtagaagtt tatgcatatg
    16681 aatatgaggc agagggtaag attgtgtatg ttaatttaaa agaaaatact attaatataa
    16741 gaaataataa tattaagcta gaaaatagta gtcagtatga ggtgctatct acatcagaac
    16801 ttaaaaaagt aaatgaatca aatgagggaa ttacgtatca agtaaaagta aaaaattcat
    16861 catcaaaaga tttaggaaat attattctta agacagcaga agttaatgaa aatggagaat
    16921 atttaacagt aaatcgtgta ccatcatata agattttaaa gccttcagaa gagacagata
    16981 tagatatatt gtgttcaaca aaggcaaaaa gtgttgaaat agtgggatat acgtatgatg
    17041 atataaaaga aaaagccaat gtggatattg acttaaaatc tcataaagtt aagatagaca
    17101 aataaaggta ggggatactt atgaaggttt taatagttga agataataag atacttttag
    17161 aaagtgtagt agaagaactt agcaagcatt ttgaaactga aaagtgtgaa gatggagaag
    17221 aagctttata cttaataaat caaaatatat atgatttagt cattttagac ttaatgcttc
    17281 ctaatataaa tggatttgat atcctaaaga aaatgagaat taataatata gatacacctg
    17341 tacttatatt gacagcaaaa gaaacattag atgacaaagt agaagcattt actataggag
    17401 ctaatgatta tttgacaaaa cctttttata tggaagagtt agtagctagg gtatacgcta
    17461 tattaaggac taatggaaag ataaaagaaa gaaacggatt agaatttaaa tctttatatt
    17521 tagatacact agaaaaaaga gtgtatattg aaaaagaaga aataaaactt caaaacaaac
    17581 agtttaactt gcttgagtat tttgtattaa ataaaggttc aattttatta aaagagcaga
    17641 tttatgatag aatatgggga attgattcag atgcaactat tgaaatagtg gaagtatatg
    17701 taagtaatct aagaaaaaag ctaagtaaat atggatatga taaatatatt aaaactaaac
    17761 gtaaggtagg atatatattc gatgataaat aagaatgtat ttaccagtac taaaaatcat
    17821 ttaataaaaa tgtatattat tgtagtaggt agttttttaa taatcttttc tatatttata
    17881 tactcatatt ttaggggact tacatatagt ggaattgata gtgagataaa tgatgaactt
    17941 gaatatatag tctctcaatt taaaagaact tcatttttga acccaataag actcaaagat
    18001 cctaaagata tggtgtatgt atatgaagat ggaaggataa gttactatac tcaaaatgaa
    18061 tattttgatg agttacttcc agatagaaga ctagataaaa aaaattcgtt ttttaaatac
    18121 acagaaaatg gatatacttt tagagaatta aatgtagatg taggtagata ccaaatacag
    18181 ataattagaa atatagattc tgagatgaat tctttaagac agcttacatc tgttcttata
    18241 attggaatat taatttctgt aataattact tactttgtgg ctgtatatct cactagaaaa
    18301 gctttaattc caatagaaac agcatggaaa aatcaagcta agtttataca agatgcttct
    18361 catgaattaa gaacaccaat aacaatagtt tcatcaaaat tagaaagtat gttaaaatcc
    18421 ccagaaagta cagtaaatga tgaagttgaa acaatagcta ctgcaatgaa ggaaactaga
    18481 agacttaaaa aaatgataac tgatttattg tctcttacta aagaggactc tattgttaaa
    18541 gttaatttag aagaaataga tttagaaaag ttattggaag aaatatcaga agactatatt
    18601 gacattgctg agtttcagga aaaaagattc gtgtttaatt ctaaactgaa gaataaagtt
    18661 ataataacag ataaaaataa attaagacaa cttatattaa ttttcattga taatgccttt
    18721 aagtatacaa aattgggtga tgagatagca ttagaattga aagaagatat agaggatgag
    18781 gtaactttac ttataagtga tactggtata ggcataaaaa aagaagagat acctcttata
    18841 tttgatagat tttttagaag tgaaaatgta agaaataaag atttagaagg ttctggaata
    18901 ggactttcaa tagctcgcat gattagttta aacttatcta ttgatataaa tgtaacttct
    18961 gatgtagata ttggaactac atttgaattg tcaataccaa aaaagttaaa ataataaaaa
    19021 tataaaaaat tgtttttaga aatgaagaat gggtatacta attagtataa aatataaaaa
    19081 gaccttataa aagtaaattt atatttataa atttcatagt ataattatat agggataata
    19141 aagtttaata ggaggattta aaaatggcaa aagttataaa tacatctgaa tttagaagta
    19201 gtgttgaagg tagtaaggga gtagttgtag ttgatttctt tgcaacttgg tgtggacctt
    19261 gtaacatgtt gggacctgta tttgcagaat taggtgaaga gatgaaggat aaagctagat
    19321 ttgtaaaagt tgatatagat gagagtttag aaatagctca acaattcaat gtatctacag
    19381 taccaactat gattatattt aaagatggaa aaccagtaga aactttaatt ggattcatgc
    19441 caaagaacaa aattgaaatg caagtaaaat catatttata aaaaatgggt gagtattatg
    19501 agatatgata tagctataat cggcagtggt ccagctggat tgtctgctgc tataaatgca
    19561 aaaataagaa ataaaacaat tatcatgttt ggtaatgata atttaagtaa taagcttgtt
    19621 aaagctcctt caatagataa ttatttaggt ttttatgata taagtggtga tgaattaaaa
    19681 gacaagttta aatcacatat agatagtatg gatatttcaa ttgaaaataa aagaataaat
    19741 aatatttatg ctatgggaga atactttaca ataatgtctg gaaatgatat gtatgaagct
    19801 actactgtaa tccttgcaac tggggtagag tatactagac caatcaaggg tgaagaagaa
    19861 tttctaggaa gaggtgtggg ctactgtgct acatgtgatg caccacttta tagaaataaa
    19921 aaggtagcag ttataggata taatgaagaa tctaaagaag aagcaaattt tttaagtgaa
    19981 ctcacttcaa agacttattt tatacctatg tacaaaaaag ataatcttat gagaagttca
    20041 gataacctag atgattctat tgaagttata catgacagac cagtacagat agatggagat
    20101 aaacttgtaa acaaagtatc ttttaaagaa aaccacattg aagttgatgg agtattcgtt
    20161 ataaaagata gtacggctcc aagtgcattg gttcctggaa tagagattga tggcattcat
    20221 attaaagttg acaataatat gaagactagt atagatggat gctttgctgc tggagattgt
    20281 gttggaaaac cctattctta tataaaagca gctggtcaag ggcaaatagc agctttaaat
    20341 gctgtttact atttagataa attaaagagg gcttaatatt tatattgatt aactttttaa
    20401 agatatttat aaaaaactgt cttgaaataa ttttattatt tcaagacagt tttttatttt
    20461 taagttatta ataaaagtat tttaaaatct atatgtaatt acttttatta ataaaactca
    20521 aagttatatt agtaaaaatt ggaattattt ataatatact ccaaaattta aaaataatgg
    20581 ttgaaaaatc taattaagag tactataatt taattaagta attacttaat caaaatgagg
    20641 tacttatgga aaacttggtt aaaatattta aagcattgag tgatgaaact agacttaaca
    20701 tacttatatt agtatccaag agaaatatat gtcaaaaggg gatttctaaa tatctaggga
    20761 tttcagattc tgctgtttca caacatataa aaatattaaa agatgttggg ataataacag
    20821 gatataaaga aggatactat gttttatatc atattaataa agaatcattt aatatatgtg
    20881 tagattttat aaattctatg ttaagtaatt ctagtgaaag ctttatagat gtatttgatg
    20941 taaatacaat acatctagga tgcagtaaag aatgtaaatc tataaaaaaa tgttgtaaga
    21001 gaagggagaa ataataatga aagtatgtat accagtagag gagaataaag gattggatag
    21061 taaaccatat ggacattttg gttctgctcc aatttttgtt gtgtgtgatt tagaaagtgg
    21121 agaagttaaa tctttagata atggagattt agatcatgaa catggaaaat gtcaaccatt
    21181 aaaagcattg tcagggacag ctgtagatgc agtagttgtt ggaggaatag gacaaggagc
    21241 tataattaaa ttaaatggaa tgggaataaa agtttacaga gcagaaggtg atactataag
    21301 tgctaattta gacttactta aaaatggaaa attagtagaa tttccatcag accatacttg
    21361 ctcacatgac ggatgtggac atcattaaaa aaaaacagct gatgaaatat atacttcatc
    21421 agctattttt tcttttaata aatacgattt tattctttta aaaccaaaat ttagattttt
    21481 atgttttttg tagtatcctt aataatattt caagtttaca agtttactac ttttagattt
    21541 tttttgtatg tttttctata tagtcaatat catttaatat gtcattgagt tctttattaa
    21601 gtgtttttaa atcatcacca tctacattgt agtttaaaaa tagttcctct acattatcaa
    21661 tgatagaatt gacttttcta gtaacttcaa caaatgtctt aaagttacta ctatttttag
    21721 catttagaaa tttttttaaa tcctttgata taaatgatac tatttcatat tcatcattaa
    21781 atagacctct accaatagga tatggttcaa ttctctgtaa aaaatattgg tctttagcat
    21841 taatattata agtataagat atttcccaac cgtctcctaa atcaaacttg ataaaatata
    21901 atgtactact tacttttgat attcttgtgg caccaagctg tcttaaaaga acttcaatcg
    21961 aatcaccttg aacagtatta atattcatat atacacatcc cctttagtat tattatacta
    22021 tattttttaa aattttataa ttaaataaac agtagatata ataatagata taatcataag
    22081 tggaaaacct attttgaaat actccttaaa agagatagga tgaccgtgtt tattacctat
    22141 acctgctagt acaacattag cagatgctcc tattagtgtt ccatttcctc caagacaagc
    22201 tccaagagat gttgcccacc atagtggcat tacatcaata ccttcagctt gcatagtcaa
    22261 aataagagga attagtgttg caacaaatgg aatattatct aagaaagaag agactatagc
    22321 tgacaaccat aatatcaaca acattgtaaa tacaagatgt ccttctgtaa gtccaatcaa
    22381 tgcttgagcc aatttgttta ttatcccaac ttctactaat ccaccaacta ctacaaataa
    22441 tcccataaag aataaaattg tagaccattc aatacctgcc ataatttcat ctacatcttg
    22501 ttttcctatt aaaagcatta tagatgctcc tgtcagtgct acaacagaag aatctatgtg
    22561 gattgtactg tgaaacataa aaccaaaaag tatgaatgct attaatataa ggctttttat
    22621 aagtagaggt ttatctttaa cagatttttt ttcatctagt tttaatattg catttttagc
    22681 tctttcattc acaacaagtt cttttccata aataaatcta aagcatatta tagttatagc
    22741 aagtattact ataacagcag gacctaaatt tattacaaaa tccataaaac taagattggc
    22801 agcactacca atcataatat ttggagggtc tcctataaga gtagctgttc ctcctatatt
    22861 tgaagctaaa atctgtgtta ttaaaaatgg aactggatta agtcctaata tttgtgtaat
    22921 aactatagtc attggtccaa taagtaagac ggttgtaaca ttatctaata cagctgataa
    22981 aatagctgtt ataatagcaa aacaaatcat tatcttccat gggtcacctt tggctttctt
    23041 tgcagtccag atagctatat attcaaataa ccccgagttt ttgacaacag ctacaaatag
    23101 catcatacca actaatacac caatagtatt gaaatctata tggcttaatc cttcatcaag
    23161 tgtaagtata ttgaatatta tcattaatag tgcaccacca acagctgcta tggatctgtt
    23221 taccttttct gtaataatag ctgccataac tattaaaaat atacatatag ctattatttg
    23281 ttgaaattcc ataatataag tacctccatt tgtattattt tgtgcctaat gctagtacta
    23341 tatatagtat tactttttta ttgttttttc aatggatgta tgaaagtata ttatttaaca
    23401 aagtttttgg aaaattgaca gaatttaaag aaaatttttg ttatttaaat aaattttaaa
    23461 attattccat tgataataat attaaacgac aaacgaaaaa atataccctt taaactagaa
    23521 tttattgtat ctaataacaa ttataaaata ttaaatatat ctatattata aa
//
LOCUS       DAGSRM010000054        23440 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.054, whole genome shotgun sequence.
ACCESSION   DAGSRM010000054 DAGSRM010000000
VERSION     DAGSRM010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23440
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.054"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..946)
                     /locus_tag="MJE33_002305"
     CDS             complement(<1..946)
                     /locus_tag="MJE33_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HBY9648237.1"
                     /translation="MYILNLNTRETIEDFRDKFYVAENSYLILSAPKNLKLLKETLDI
                     DEITFNDCLKFDEITKLDLFDNYDFLSLNTFELRDGEAVIEEVNMYLSDNFILVVVNE
                     EHFLFEFVKNIILKNSQLEKNPVINLFKINYLILREVIKNGFESLEKVEELILQIEDE
                     MMDNINKNHVSRISDVRGLTRIIVKNTRPLLYIGDRIVKENIRYLKYSNVKKYNLENF
                     QGIDFGIDKLYNFALSTRELADKLLDIYSSRVGEKTNNLITKLTLLTAISAPLTIITG
                     IYGMNFRYMPELNWIYGYPATLFFMLCIIFVGIIIFKIK"
     gene            complement(1303..2001)
                     /locus_tag="MJE33_002306"
     CDS             complement(1303..2001)
                     /locus_tag="MJE33_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY9648238.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEAQQKDDVKSTFEVDKLLIDFDKRKVIVDDVEVHLTP
                     IEYNILSLLAKHHGKVLTHNFIIKEIWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDE"
     gene            complement(2035..4737)
                     /locus_tag="MJE33_002307"
     CDS             complement(2035..4737)
                     /locus_tag="MJE33_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HBY9648239.1"
                     /translation="MDRPNPDILLEKIKNEEEKLSRGQLKIFFGYAAGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKDIEYKSIKLKEFDLDAALARK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEIITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRIAKL
                     RNVTKIVIGRNHSSNKRFSKRFKKDIVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     SSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETAAKREENTKMIYQIIKGFLRLSNKDDIVNKGIELLSLSLSRDVICYLNVDKHNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCIDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDTQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVENNKDKGSTFKFSLPKENE"
     gene            complement(4915..6732)
                     /gene="ftsH"
                     /locus_tag="MJE33_002308"
     CDS             complement(4915..6732)
                     /gene="ftsH"
                     /locus_tag="MJE33_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBY9648240.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLSILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALTEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFEESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKACH
                     KNAIEILENNIDKLHELANYLLEKETITGDEFMEILNLN"
     gene            complement(6763..7206)
                     /locus_tag="MJE33_002309"
     CDS             complement(6763..7206)
                     /locus_tag="MJE33_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBY9648241.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLSDFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQEHISSSIFDSYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            complement(7697..8587)
                     /gene="metA"
                     /locus_tag="MJE33_002310"
     CDS             complement(7697..8587)
                     /gene="metA"
                     /locus_tag="MJE33_002310"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBY9648242.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKELKYEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTGFNVSVEFIKISTRESKRSCNEYVKEFYKTFDEAKGE
                     YFDGFIITGAPVEQMEFEEVDYWNELEEIMDYSKRKTKSTLYICWAAQASLYKYYNVK
                     KLPLSQKCFGVFKHKVDKDSKIVDGFENEFFAPHSRHTTVNIEALKENKELSIVSHSK
                     EAGPYIITNSRDVFVMGHSEYDKYTLDKEYKRDINKGDKISIPQNYYINDDPSEEPTV
                     KWKKHSELLFRNWIKNYLIQ"
     gene            complement(8617..9882)
                     /locus_tag="MJE33_002311"
     CDS             complement(8617..9882)
                     /locus_tag="MJE33_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HBY9648243.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLEDNKYYENAKKYLSRG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENVEDLIADLNQALNF"
     regulatory      complement(9992..10094)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(10148..12805)
                     /locus_tag="MJE33_002312"
     CDS             complement(10148..12805)
                     /locus_tag="MJE33_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBY9648244.1"
                     /translation="MENHKQMPWHSRATHEILESLDSSEEGLTDTEAEKRLKENGLNE
                     LRKKPPRTTLQMLWSQITDAMVMILIGAAILSLIFGEFTEAFVILIIVTVNAVIGIVQ
                     EKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVVGDIVFLEDGAMVPADLRLIET
                     SSLKIQEASLTGESVPSEKDADTILPKECVLGDRSNMAYTSSIVTYGRGVGVVVATGM
                     DTEVGNIAHLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGAFYQRPLLPMF
                     MIAISLAISIIPEGLPATATIVMALGVQRMAKRNALIRKLPAVETLGGATVICCDKTG
                     TLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCNDACINPDKTD
                     EILGDPTEGALIFMAKKFGIDQEALEEEYPRVFEQPFDSDRKCMTTVHNIDEKIIAYT
                     KGAVDEILDLCTKILTSKGERNITEVDKKNIHELCLNMSKDALRVLGFAKREISSIPK
                     EDSENIEYDLTFIGMVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHKVTATTIAHEL
                     GIWSEENTVISGDELDNLSDDELDEAVKNTTVFARVSPFDKLRIIQSLKRIGEVPAMT
                     GDGVNDSPALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTIAYAIKEGRRVYRNIQ
                     KVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALALGVDPPSRNI
                     MKHQPVKSTTLFEKNLIQRVVTQGVFVAIMTILAYKIGLNIENHSVGQTMAFCVLAFS
                     QMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSAFLIAVILFVPVFRNAFNLTVLNNL
                     EWLITIVLAIMSVIQVEVGKIIKRKKLKK"
     gene            complement(12933..13676)
                     /gene="yaaA"
                     /locus_tag="MJE33_002313"
     CDS             complement(12933..13676)
                     /gene="yaaA"
                     /locus_tag="MJE33_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HBY9648245.1"
                     /translation="MIIMLSPAKNMKNIEVFDRDLSLPCFIDNTKEIVENIKTFGIED
                     FKNKMKINEKLAVLNKNRFESIKFDRLGNPAILTYDGIQYKNIEAENFTRKDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQTKLQVGEFKNLYEYWGNRIYKELIKEKTAIVN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGILKVESTQAKKARGMMTKYIVKNRIRD
                     IEELKKFDLEGYKYKENLSNNSEYIFVKE"
     gene            complement(13686..14150)
                     /gene="bcp"
                     /locus_tag="MJE33_002314"
     CDS             complement(13686..14150)
                     /gene="bcp"
                     /locus_tag="MJE33_002314"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HBY9648246.1"
                     /translation="MLSIGTKAPEFTLEDKDGNKVSMSDFKGKKVVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDIQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEKQE"
     gene            complement(14502..16199)
                     /gene="ade"
                     /locus_tag="MJE33_002315"
     CDS             complement(14502..16199)
                     /gene="ade"
                     /locus_tag="MJE33_002315"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBY9648247.1"
                     /translation="MGNLKRQCDVSSGREKADIVLKNGTIINVFTEELITGDVAIVGD
                     TIVGIGDYKGNVEIDCSNKYISPGFIDAHMHIESTMVMPIELSKKLLKSGTTTIIADP
                     HELVNVKGVSAIDFLLESTKDIPLNVYIMVPSSVPATSFETNGVGKFSAKDMESYVNN
                     PRILGLGEVMCFNDVINSENEILDKLELFKNKVVDGHAPNINGKSLQTYVCAGIENDH
                     ECITFDEVYEKLRAGLKILIREGSAAKNLKSIVSGMLKHNLPIEEFMFCTDDKHLDDI
                     EKQGHIRWNIKCAIDLGMEPVRAIKVATYNSAKAYGLRKIGAIGAGYKADIVVLNDLD
                     KMEVDSVYKDGNLVNEEMFSNYNYEIKDKELLNTVKFKYINKEKIQLKVSEKNYVMEI
                     VPYQILTNKVYESLPCADGYFVPNKEYSKLCVVERHRMTGNVAIAPLKGFGIKNGAIA
                     TTVAHDSHNIIVAGDNDDDILVAINYLKEIQGGYVIVSNGKVLAHLSLQVAGLISTFT
                     AEEVQEITDNMLEIARKMEVPEYVDPFITLSFMALPVVPQIRLTDLGLFDVEEFKFI"
     gene            complement(16505..17659)
                     /locus_tag="MJE33_002316"
     CDS             complement(16505..17659)
                     /locus_tag="MJE33_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic efflux protein"
                     /protein_id="HBY9648248.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDISKNNPKENKEQHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKIYIGIIVIGFIFMSLAHSGLNLPIIEKLHSL
                     EEVIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMIITTIPAILVGMIVYAFLG"
     gene            complement(17904..18743)
                     /locus_tag="MJE33_002317"
     CDS             complement(17904..18743)
                     /locus_tag="MJE33_002317"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HBY9648249.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFDNSKRYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKEVDCMIVIGGK
                     HSSNTQKLVKVCEDLVPTFAIETKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            complement(18756..19352)
                     /locus_tag="MJE33_002318"
     CDS             complement(18756..19352)
                     /locus_tag="MJE33_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBY9648250.1"
                     /translation="MNFYRFVINIFKGFSKIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAIENREIAAIAKKELFKVKPLGFILKKLHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLSNDDHERISQEILEVIKQNYFKYSK"
     gene            complement(19403..20053)
                     /locus_tag="MJE33_002319"
     CDS             complement(19403..20053)
                     /locus_tag="MJE33_002319"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBY9648251.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGIDVNNEKEVIQIAENSDIDFKDNNIYLDKEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVEVQRKIGMKNSVILDGRDIGSYVFPDADYKFFLVATPEERGNRRYKELCNKG
                     YNTTLEEVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVVSKINL"
     gene            complement(20099..21352)
                     /locus_tag="MJE33_002320"
     CDS             complement(20099..21352)
                     /locus_tag="MJE33_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBY9648252.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNREIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEEENDHK
                     VYTKNDKSIEVIEVLNKDLLNHNVKIMYNKKVIDIITEEIALKDDSNKDKSKYLIKGI
                     ILDNGDKILGDKVIISTGGVSYSKTGSDGSMYKILKKHGHTLNKLYPALVPLTIEEKW
                     IKDLQGISMKNVEISCKIKKRKISKSGDMLFAHFGITGPCVLIMSSYINKIIEKEKVE
                     LNIDFLPNLSTDEISSIIRAFPNKNVLNNLKQILPQNFLKEIFSLLSLVDKKASDLSK
                     ADEIRIIEYIKNMKLTCNGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            complement(21376..22017)
                     /locus_tag="MJE33_002321"
     CDS             complement(21376..22017)
                     /locus_tag="MJE33_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBY9648253.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILGDKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIVKMNDTSHIKNHVKINNSALE"
     gene            complement(22219..22632)
                     /locus_tag="MJE33_002322"
     CDS             complement(22219..22632)
                     /locus_tag="MJE33_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HBY9648254.1"
                     /translation="MLELKNNLGISERKLKYPCIYKHFKGKYYATMGLSKAIDDIENI
                     CEIYGKENLIQNRNKYKLVIRHTEREEDIYVYRDLDGNFYHKKEEDTNDLVLYKTLYD
                     DTGIFARPLDMFLEKVDTDKYVNSIQEYRFEEVYK"
     gene            complement(22783..23124)
                     /locus_tag="MJE33_002323"
     CDS             complement(22783..23124)
                     /locus_tag="MJE33_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HBY9648255.1"
                     /translation="MKEENFINVEYEDKRFSINSKEVLYIEKQEYEVKIYTESGNYEV
                     GENAPCIRKLLKEENFFKCSNSYIVNLDKCEFYLSNNCIFVNNNFIRILGSERKKKLK
                     KKLEKLYDYED"
BASE COUNT         7415 a         4160 c         2471 g         9394 t
ORIGIN      
        1 tttttatttt aaatattata attccaacaa aaattataca tagcataaaa aataaagttg
       61 ctggatatcc ataaatccag tttaattctg gcatatatct aaagttcatt ccatatatac
      121 cagttataat tgtaagtggt gctgatatgg ctgttaacaa ggttaatttt gtaattaagt
      181 tatttgtttt ctcacctact ctggaagaat aaatatctaa taatttatct gctaactctc
      241 gtgttgacaa tgcgaaattg tataatttgt caattccaaa atctatacct tgaaaatttt
      301 ctaaattata tttttttaca tttgaatatt ttaaatatct tatattttcc ttaactattc
      361 tatccccaat atacaaaaga ggtctagtat tcttaactat aattcttgtt aatcctctta
      421 catcacttat tctagataca tgattcttat taatgttgtc catcatttca tcttctattt
      481 gaagaattaa ctcttcaact ttttccaaag actcaaaacc attttttata acttctctaa
      541 gaatcaagta gtttatctta aatagattaa taactggatt tttttcaagt tgagagttct
      601 tcaaaataat attttttaca aattcaaata aaaagtgttc ttcattcact acaaccaata
      661 taaaattatc tgataaatac atatttacct cttctataac agcttctcca tctcttagtt
      721 caaatgtatt taggcttaaa aaatcgtaat tatcaaataa atcaagtttg gttatttcat
      781 caaatttaag acaatcatta aaagttattt catctatatc caaagtttcc ttaagcaatt
      841 ttaaattttt tggagcactt aatataagat aagaattttc tgctacataa aatttatctc
      901 taaaatcttc aatagtctct cttgtattta aattcagtat atacatacat ctactcctaa
      961 atttcaattt acattctaaa aattttttat aatccttact gtaattattt tacactattt
     1021 taataattat tatttaatat ttatattaaa tttattataa ggtaagtgtt aaatacattt
     1081 attatttaac caaagttttt attacagatt acactatata cttattttaa aacatatttc
     1141 atattattga tactaaaaaa ttattgatac taaaaaagct aaaaaataaa atgcatgtgc
     1201 taaactgaca acaagcacat acacttcatt cttattttaa ctaaactata taattttaat
     1261 atatattaat aagcacataa tctaataatt aatatattac aactattcat catttaatct
     1321 gtatcctaca ccaacttctg taattatata tcttgggttt gcaggttgtt tttctatttt
     1381 tcgtcttaac gttgccataa agacccttaa agacttagtc tcattcccta taacactacc
     1441 ccatatttct tttattataa aattatgagt aagcactttt ccatgatgct ttgcaagtaa
     1501 tgaaagaata ttatattcaa taggtgtcaa atggacttca acatcatcaa caataacttt
     1561 tcttttatca aaatcaatca ataatttatc tacttcaaac gtagatttaa catcatcttt
     1621 ttgttgagct tctacttgtg aatgtctaaa tgctactctt actcttgcta ataactctac
     1681 tatggagaat ggttttgtta agtaatcatc tgcccctgca tcaaatactt ctaccttgtc
     1741 tctatcttgc tctctagctg aaactacaat tattggtata tttgaaaatt gtctgatagc
     1801 ttttattacc tctattccat ccatatcctc taagcctaaa tctaaaagaa caacatctgg
     1861 agaatatgag acagctatag atataccttc ttttccagtt atagcctcct tgacatcata
     1921 atcctgtgtt aaaagtgcag tagatataaa ttttcttatt gtcttatcat cctctactaa
     1981 caatataagt tttttactca tttcttcctc caaaactaat tttaaaattt aaaactattc
     2041 attttcctta ggcaaactaa acttaaaagt tgaaccctta tctttattgt tttctaccat
     2101 aatttcacct ttatgagctt gaattataga tttacaaatt gataatccaa gtccaacacc
     2161 ctttctggaa tctttagctc cttcttctcc agtaaaaaat ctgtcaaaga tatgcttttt
     2221 aagttccttt gatataccag gaccattatc agatacttca aaccaaacat aatcatcttt
     2281 ctcatataca tttatctcta tcttacaatc ttcctttgaa tatttcaaag aattatctat
     2341 taaattaatt aaaacttgtt ctattaattt tgcatccata ggaacaaata atatctgttc
     2401 tggtatatta atatctataa gtgcattttc aattcttttt tttatttttt gtaacgcttc
     2461 tgatataatt tcctcaacca gttcagaatg ttttttaact tccaatttcc cttcatctat
     2521 cttggtcata ctaagtaaat tttcaacaag tcttattaac cactgagtat cttcatatac
     2581 accactaagt aattcatcta ttatatcttg acctatagta cccttgtttt ttataattgt
     2641 acttactgca ccagatattc ctgctagtgg agttctaagg tcatgcgaaa ctgctcttaa
     2701 aagattactt cttaatcttt ccctttcaat ctcaagattg gtattttctt tagcttctga
     2761 caagatttct ctatcaagcg ctattgccat ttgagcaatt attgtttcaa ttaagcttat
     2821 atcttctgta tccaattttg aatcaataca tgatattccc actactccta aagttaaatt
     2881 cattcctaca attggtatat agtatccctt tgaaccagga agtgtatccg tgtcatttcc
     2941 tgccactgtt gaattggata aaacccaact tgcaacagct ttttcatctt cactatccaa
     3001 atcattttta tccttattat tagtattttt tttgtaaagc tttgatttat tatgcttatc
     3061 tacatttaaa taacaaatta catctctact taagctaaga gacaaaagtt ctataccttt
     3121 gtttactata tcatctttat ttgataatct taaaaatccc ttaattattt gatatatcat
     3181 ttttgtattt tcttctcttt tagcagcagt ttctgcttca tgctgtatct tagacgtaag
     3241 agtgctagtt attataccta ctattgagaa tacaaccagt gttactatat agtttgaatc
     3301 tgctatagat aatgtatata atggtgctgt aaaaaaataa tttaaaagaa ctatattaaa
     3361 tacagaactt attatacctg tactataccc tttagtccac atagaaacta aaactacacc
     3421 caacatatat attaacaata tattttctct tataaatccc atgctttgta ctgcatatgc
     3481 taaaatactt atcatcaaag taataaatat aagttttata aaatcaactt tgctaatttt
     3541 aaatttagat tttattgatg atttatcctt tttaggtttg tatttaacat ctgaggcaaa
     3601 tttatatgga attatatgta tatctatata atcaacttca tcaattaatt tgtcaactat
     3661 atctttttta aatcttttac taaatctttt attacttgaa tgatttctac ctattacaat
     3721 ttttgttaca tttctcagtt ttgcaattct aagtatttgc tctattatat tttcaccatg
     3781 taaaacaatt atttctccac ctaatttctt agctaatttt aggttatttt gtagttgttt
     3841 ttgttcatca aatggaagtc ttgctgtgtt tggagtttca acatataaag ctatccattt
     3901 tgcaaatgta ctgtctgata ttctggatgc agttctaata acttttgctg aagatggaga
     3961 tggacttata catgcaagaa gtgtatctga tgtaggtatt acagttacat caccctttga
     4021 tagtctggtc atttgaactt ctttattaac tctctctgct gtttttctaa gtgcgatttc
     4081 tctaagtgca accaagttat cttttataaa aaaattattt tttgctctag tagcttgttc
     4141 tttcttatat atcttacctt cattgaacct ttcaagaagt acatcaggtt caacatctat
     4201 aagttctaac tgtgtatttg tatccaaaaa cttatctggt atagtctctt taactgatac
     4261 gtttgtaatt atctcaacaa tgtcatttag actttctaaa tgttgtacat ttacagtagt
     4321 gtagacattt atgcctgcta acagcaattc ttctatatct tgccatcttt ttgtatgcct
     4381 taacccagca acattagaat gggcaaattc atctactaat ataacttctg gttttcttgc
     4441 taaagcagca tctaaatcaa attcttttag ttttatagat ttatattcta tatcctttac
     4501 aggaagaatt tccaagccat caagtaaagc caatgtttct ggtctagtgt gtggttcaat
     4561 atatccaact actacatcca cacccacttt ttttaaattt tgtgcagatt ccaacataga
     4621 gtaggttttt cctactccag ctgcataacc aaagaatatt tttaattgac ctctacttag
     4681 tttttcttct tcatttttaa ttttttctaa tagaatatca ggattaggtc tatccattaa
     4741 aaaacctcct tacaccattc attactatta taatatcaat tccgatataa aattgtagtt
     4801 aagatgtatt tttaatgatt aagattatgt aaagatttaa atatgaattt aaaaaattta
     4861 gttgtaaaat aaaaatgcct aacaataggc atttttatct tgtgtataat cattttagtt
     4921 taagttaagt atttccataa actcatcacc tgtaatagtt tctttttcta ataaataatt
     4981 agcaagttca tgcaatttat caatattatt ttctaagatt tcaattgcat ttttgtgaca
     5041 agcctttact attttaaata cctcttcatc tattttagca gcagtgtcgg aagaacaagc
     5101 aagagaagta tctcctccta aatattgatt gcttacagtt tcaagagcga ccatatcaaa
     5161 ctcttcactc ataccaaggc gagtaaccat agctcttgca attttagtag cttgttctat
     5221 atcatttgat gctcctgatg tacaagtgtt aaagataacc tcttcagcag cacgaccacc
     5281 tgtaaaagta gttattttat caattgcttc ttctttagac ataagtacac tctcatcttc
     5341 agcaacttgc atagtatatc ccaatgctcc tgaagttcta ggtattatag taatcttatg
     5401 gacaggtgca gaatgttttt gcatagcagc aacaagtgca tgacctattt catgatatgc
     5461 tataattttc ttttccttat ctgaaattac agctccctta cgttgatacc cagcaattac
     5521 agtttcaaca gattcttcaa aatcctgttg ttctactata tctctgccag acttaactgc
     5581 taaaagtgct gcttcgttta caatatttgc aagttctgca cctgatgctc ctgatgtaga
     5641 ccttgcaatt gcattataat ctatatctga gcttacattt acattttgag catgtacctt
     5701 taatatagct tcacgtcctc caaggtctgg aagttcaact ggtatacgtc tatcaaatct
     5761 tcctggacga agtaatgctt tgtcaagact gtcaggtcta tttgtagcag caagtattac
     5821 tactccaatt ccaccatcaa aaccatccat ctcacttaac aactgattta aagtttgttc
     5881 acgttcatca tttccaccta atccacttcc attatcacgc tttttaccaa tagtatcaat
     5941 ctcatctata aacactatac atggagcttt ttcttttgct tgtttaaata aatcacgaat
     6001 cctagaagct cccattccta caaacatctc aacaaactca gaaccagata ttgagaaaaa
     6061 tggtacattt gcttcacctg ctacagcttt tgcaaggagt gtttttcctg tacctggagg
     6121 tcctactaaa agtgctcctt taggcatttg tgctcctatc tttttatact tttctggatt
     6181 gtgcaagaaa tctactattt cagttaaagc ttcttttgcc tcatcttgac cagctacatc
     6241 attaaatctt tttccacttt gagcagatac atacattttg gcgttgcttt ttccaaattg
     6301 catagcatta ccacccattc gcttttgcat agacttcatc attaagttac caaatagcat
     6361 aagaatacct ataggtatta tccatgtaag taaaatactt aaaagaggtg aattttcctt
     6421 aggaataacc ttagtaaatt ctacttttgc attttttagt ctatctacca attctgggtc
     6481 atccattcta ccagttatgt atatattctt atccttgcta ctagatggat ttattgctat
     6541 tctattattt tctatctcaa cagatttaac ctttcctgta tccaacattg tgagaaactt
     6601 tccatagtca atctcaacca tttttttatc cattaaactt ggaaatatta aagcatttaa
     6661 aaacattact actattatta taactgtata ataatagagt gctgaccttt tttggggttt
     6721 atgttcatcc ataattttat tctcctttaa attattttta tgttatttgc aatcaatgta
     6781 agttaaaagt ttatttaatt tctctaaacc ttctattata tcttccatat cttgcaattt
     6841 ttcactggtc atattttcaa aataactatc aaaaatacta cttgaaatat gttcttgtat
     6901 atccaatgat ttatttgtaa cttttacttt tacaattcgt tggtcttcta tatctcttat
     6961 gcgatttaaa aagccatttt tttcaagcct ttgacagatt actgatacat tactatttgt
     7021 catgtttaag ttcttcccta aatcgcttat ttttttctct ccatgttcac ataactcaga
     7081 taaaatcaaa gcttgcactg ctgtaatacc aagtggctga taaaaatcac ttaataaagt
     7141 tgtaaatttc atatttatat ctctagtcaa ctctagtagc tctttcttaa aactcatctc
     7201 atccatacaa acgctcccta cgaaaatact ttatttcaaa tttaggctga agtgcatact
     7261 gtaaataaat acttaacact ataatgttta atgtattaaa cattatagta ttaagtattt
     7321 attgtcaatt acaatattgt aacttcaatt ggaattttgt tatcttattg atatttaatt
     7381 ttatacagga tatttttaat aaaatttaat aaaaaatcta tatatatatt ttacactgat
     7441 ttctcccttt ttaatctctt ttaaaagtta aaatacaaat ttattgatac tataaaggtt
     7501 tagtctccca ttatcgcacc atatataaaa ttaaagccac ctctatctac atacacctaa
     7561 agtgattttc ttaaaatata aaactactat tataaggtca aatttaaaaa aaataaggta
     7621 taaagtatca ttaagattaa ctttatacct ataaatttct atattttttt agttaacatt
     7681 tatatactct taaaatttat tgtatcaaat agttttttat ccaatttcta aacaacaatt
     7741 ctgaatgttt tttccatttt acagttggct cttctgaagg gtcatcatta atatagtaat
     7801 tttgaggtat agatatttta tctcctttat ttatatccct cttatattct ttatcaagag
     7861 tgtatttgtc atactcgcta tgacccatta cgaacacatc tcttgaattt gtaattatat
     7921 agggaccagc ttctttagaa tgactcacaa tgctcaactc tttattctct ttcaaagctt
     7981 caatatttac agtagtatgt cttgaatgtg gagcaaaaaa ttcattttca aatccatcta
     8041 ctattttact atctttatct accttatgct taaatactcc aaaacatttt tgacttagtg
     8101 gaagtttttt tacattgtag tatttgtaaa gacttgcttg agctgcccaa catatatata
     8161 atgtagattt agtttttctc ttagaataat ccataatttc ttctagttca ttccaataat
     8221 caacttcttc aaactccatt tgttcaactg gagctcctgt tattataaag ccatcaaaat
     8281 actctccttt ggcttcatca aatgttttat aaaattcttt aacatattca ttacaacttc
     8341 ttttactttc tcttgttgaa attttaataa attccacaga cacgttgaat cctgttttat
     8401 ctatccttct tagtaaatct aattctgtat ctatctttat aggcattaga tttagtatag
     8461 ctatcttagt gtctatgttt tcttcatact ttaattcctt tttaaaatct tctttataaa
     8521 ttacatctat tccttcatct aataatttat taataacagg taatcccttt ggtagtatta
     8581 aagccatatc ttttcacttc tctttctctt agaatattag aaatttaaag cttgatttaa
     8641 atcagctatt aaatcctcta cattttctat tccaacagat aatcttatta gtgaaggcaa
     8701 tactccagat gcaattaatt gttcttctgt taattgtcta tgagtagttg aagctggatg
     8761 tataacacaa gttcttacat ctgaaacatg tgtaaccaaa gatgctatct gtaatttttc
     8821 cacaaatttt ttggcatttt ctaacccacc tcttactcca aatgataaaa caccactaca
     8881 tcctcttgat aaatactttt tggcattctc ataatacttg ttatcttcaa gtcctgggta
     8941 attaatccaa tctacatttt catgtttttc taaaaatcta gctattttaa gtgcattttc
     9001 actatgtctc tccattctaa gatgtagtgt ttcaacattt aaattagtaa gatacgcatt
     9061 gaatgggctt aaacaatttc catagtctct aagcaactga actcttgctt tagttgcata
     9121 tgcggcattt ccaaattttt gagtatagct tataccatga tatgttgggt ctggctcaac
     9181 taattctgga aattttccat tatcccagtt aaagtttcca ccatccacta taattcctcc
     9241 aacacttgaa gcatgcccat ctaaatattt tgtggtagaa tgagtaacta tatttgctcc
     9301 atactttaaa gggttacaaa gcactggaga tgctaatgaa ttatcaacaa taaatggaac
     9361 attaattctc ttagctacat ttgctatttt ttcaaaatct atgacttcaa gagttggatt
     9421 tgcaagtgtt tctgcaaaca caaccttagt attttccttt gcaagttcta ctatctcatc
     9481 ttcacttgaa tctaagtcaa acgatattaa atctatacca aattttttaa gactaggtcc
     9541 tagtaaattg aatgttcctc catatacttt tgaagaacaa agtatactat ctcctgattt
     9601 acatatattt aaaactgcca acatatttgc agattgccct gatgatacag ctacagaaga
     9661 tactccaccc tctagtaaac ttattttttc ttcaaaagct tgaatagtag gattgcttat
     9721 tcttgaatat atatgtccat caacttttaa atcaaataat tcagcaagtt ggtctatact
     9781 gctatattta aaagttgtac tttgatataa aggtagtact cttggttctc cattacctgg
     9841 tttataattt ccttgcacac atattgtttc tttattatac attcttgtat cccccttaga
     9901 ttttagtaaa aaaaattaaa ctatttacca gatgagagtt aaaataaata aagcctgagt
     9961 atataactca ggctctgtat aagcttaatt atactcatct ctcagctttc gccgcaggat
    10021 ttagcaccaa tgtatataaa tatactggtt gccgggtttc atagggccag tccctccacc
    10081 tctctggata agatttattt aatttttcta tgttttatat cttatatatt tttcctaatt
    10141 ttgtcaacta tttttttaat ttttttcttt taataatttt cccaacctct acttgaatta
    10201 cagacattat ggctaaaaca atcgttataa gccactctaa gttatttaaa actgttaagt
    10261 taaaagcatt tctaaaaaca ggtacaaaca gtattactgc tattaaaaat gcagaagtta
    10321 aaaatgagat taaaagaaat ggatttttac cattatttct attaaacatt gaatctgtat
    10381 ttgaacgctg actaaatgct cgtaacatct gtgaaaatgc taatacacaa aatgccattg
    10441 tttgacctac agaatgattt tctatattaa gaccaatttt atatgctaaa atagtcatta
    10501 ttgccacaaa tacaccttgg gtaacaactc tttgtataag atttttttca aacaaagtgg
    10561 ttgatttcac tggctggtgc ttcattatat ttctgcttgg tgggtcaact ccaagagcaa
    10621 gggctggaag cgtagcagtt gcaaggttta cccatagaat atgaacagca agaagcggag
    10681 tatcccaatt taataatatt gctacaaaca acgtaagtat ctctgctata ttaccaacaa
    10741 gcaaaaattg tataactttt tgtatgtttc tatatacacg tctaccttct tttattgcat
    10801 aagcaattgt tgtaaaacta tcgtccatta aaatcatgtc agaagaatct tttgcaacat
    10861 ctgtaccact tatacccatg gctataccaa tatcagcaga ctttaaggca ggagagtcat
    10921 ttacaccatc cccagtcatt gctggtactt ctccaattct ttttagagat tgtataattc
    10981 taagtttatc aaatggggat acacgagcaa atactgtagt atttttaaca gcttcatcta
    11041 gttcatcatc actcaaatta tcaagttcat caccagatat cacagtattt tcttcactcc
    11101 atattcctaa ctcatgagcg attgttgtgg cagtaacttt atggtcacct gttatcatta
    11161 tggttcttat acctgcttct ttacaagtac ttactgcatc aattacctca gttcttggtg
    11221 ggtcaatcat accaaccata cctataaatg ttaaatcata ttctatattt tcactatctt
    11281 cttttggtat agatgaaatc tcacgcttag caaaaccaag tacacgcaat gcatctttag
    11341 acatgttaag acataattcg tgtatattct ttttatccac ttcagtaatg tttctttcac
    11401 cttttgaagt taatatttta gtacataggt caagaatttc gtctactgct cctttagtat
    11461 aagcaataat cttttcatct atattatgaa cagttgtcat acattttcta tctgaatcaa
    11521 aaggctgttc aaatacacgt ggatattctt cttcaagagc ttcttgatct attccaaatt
    11581 ttttggccat aaaaattaat gcgccctctg ttgggtcacc taatatttca tctgttttat
    11641 ctggattaat acatgcatca ttacaaagtg caccagcata aacaatctct ttatacacta
    11701 atggatgttt ttcagatgca gtatctattg atgtcacttt tccagattca aaatctccat
    11761 tgacagcaat atgtgtaaca gtcattttat ttaaggttaa tgtaccagtt ttatcacaac
    11821 atattactgt agctcctcct aatgtttcaa cagcaggtaa ttttctaatt aaagcatttc
    11881 ttttagccat acgctgtacg cctaaagcca ttacaatagt agctgttgca ggtaatccct
    11941 ctggaataat tgatatggca agggaaattg caatcataaa cattggcaag agaggtctct
    12001 gataaaatgc acctattgca aatataacaa cgcatacaat aatacctatg acacttaatg
    12061 tctttccaac agtattaagt ttacgtttta taggagtatc aaaatcatct tgtgaatcta
    12121 aaagatgtgc tatgttaccc acttctgtat ccataccagt tgctactaca acacctacac
    12181 ctctaccata tgtgactatt gaagatgtat acgccatatt gctacggtct cctaatacac
    12241 attctttagg taagattgta tctgcatctt tttctgatgg aacagattct cctgttaaag
    12301 aagcctcttg aatttttaaa cttgatgttt ctattaaacg aaggtctgct ggaaccatag
    12361 ctccatcttc taaaaaaaca atatctccta ctacaagatt acttgctgga ataacatttt
    12421 cttcaccatc acgcataaca cgagctgtag gtgaattcat attttttaat gcttctaatg
    12481 atgattctgc ttttttctct tgaacaattc caataactgc atttactgtt acaattatta
    12541 gtattacaaa agcttctgta aattcgccaa atatcaaaga taatattgct gctcctatta
    12601 gtatcataac catagcatct gtaatttgac tccaaagcat ttgtaaagtt gttctaggtg
    12661 gtttttttct aagttcattt aagccattct ctttaagccg tttttccgcc tcggtatcgg
    12721 tgagcccctc ctctgatgaa tctagacttt ctaaaatttc atgcgttgct cttgaatgcc
    12781 aaggcatctg tttgtgattt tccattttgg aagaccactc ctttcttttt ttaatttaag
    12841 aataattata ttttaaaatc aagttcaatt caatacacac taagtatatt ttacctaaat
    12901 ttaacaatgc catttaatat ttaacaaaac ttctactctt ttacaaatat gtattcacta
    12961 ttgttagata agttttcttt atacttataa ccttctaaat caaacttttt cagttcttca
    13021 atatctctta tcctattttt tacaatgtat tttgtcatca tacctctagc tttctttgct
    13081 tgtgtagatt ccactttcaa tatgccattt ttatttactt taaaagtaca tgtaatatat
    13141 gtatcactat ctttaataaa tttttctatg gatttgctgt attcatttga tgaaagatta
    13201 acgatagctg ttttttcttt gattagttct ttatatatac gatttcccca atattcgtat
    13261 aaattcttaa actcacctac ttgtaatttt gtctgcattt ctaatctata ttcatatata
    13321 ctatcatatg gttttacaac accatacaat ccacttatga ttcgaataca gctatttgca
    13381 aactcttcat ctttccttgt aaaattttct gcttctatat ttttatattg tattccatca
    13441 taagttaaga ttgcagggtt tcctaatcta tcaaatttaa tgctttcaaa tctattttta
    13501 tttaatacag ctaatttttc attaattttc attttatttt taaaatcttc tataccaaat
    13561 gtctttatat tttccactat ttcttttgtg ttatctataa agcaaggcaa actcaaatct
    13621 ctatcaaata cttctatatt cttcatattt ttagctggtg ataacattat aatcataaat
    13681 tcttattact cctgtttttc caaatattca agaatctctt gtgcatttgt gtctggtttt
    13741 gctttttcaa agactttttc aataattcca ttttcatcta taatatatgt cgcacgtaca
    13801 actcccaatg cagtttttcc atacattttt ttctctttcc atacatcaaa agcttttata
    13861 gcaactaagt cagggtcaga aagtaaaata aaaggaagtt catgcttttc tgcaaatttt
    13921 tgatgtgatt ttatactatc tttacttatc ccaataactt gtatatcttc ctttttaaat
    13981 ccatcatatg catttctaaa agcacaagct tgacgtgtac atcctggtgt attatcttta
    14041 ggataaaaat atactactac ttttttgcct ttaaaatctg acatacttac cttatttcca
    14101 tctttatctt ctagtgtaaa ttctggtgct tttgttccta tactcaacat aaatatctct
    14161 ccttttgttt ttattaataa ttttatacta tgcttatatt attaccctat aaatctatat
    14221 ataaataaaa tattttattt tttatgtata aagtttcata aatatataat tttcatgtat
    14281 ttatatgtta aaatagactg aaaatagttt tattaagata tctatgctta aaacaatgtt
    14341 aaatgataga ttaattaaaa taaggatatc taaaaataaa atttttagtt catgagatgc
    14401 tcttttttgt atgaaatcaa atatattttc atcatttcaa caataaaaag agacttatct
    14461 ctattttgag aaagcctcat ttattattac ttttatacta tctatataaa tttaaattcc
    14521 tcaacatcaa atagacctaa atctgtaagt cttatttgtg gaacaactgg aagtgccata
    14581 aaagaaagtg taataaaagg gtctacatac tctggaactt ccatttttct tgctatctcc
    14641 aacatattat cagtaatttc ttgtacttct tcagctgtaa aagtactaat taatccagct
    14701 acttgaagac ttaggtgtgc aagaactttt ccatttgata caattacata accaccttgt
    14761 atttccttta agtaatttat agctactaga atatcatcat cattatcccc tgcaactata
    14821 atattatgag aatcatgggc cacagtagta gcaattgctc cattttttat gccaaatcct
    14881 tttagtggag caattgctac atttccagtc attctatgtc tctcaacaac acaaagtttt
    14941 gaatactctt tatttggaac aaaatatcca tctgcacatg gtaaagattc atatacctta
    15001 tttgtcaaaa tttgataagg tacaatctcc attacataat ttttttcact tacttttaac
    15061 tgtatttttt ctttattaat atatttaaac ttgactgtat taagcaattc tttatcttta
    15121 atctcataat tgtaattaga aaacatctct tcgtttacca aattaccatc tttatataca
    15181 ctatccactt ccattttgtc taaatcattc aatacaacaa tatctgcctt ataacccgca
    15241 cctattgctc caattttcct taacccatat gcttttgcag aattataagt tgcaactttt
    15301 atagccctaa caggttccat acctaaatca atcgcacatt ttatattcca tcttatgtga
    15361 ccttgttttt ctatatcatc taagtgctta tcatctgtgc aaaacataaa ttcttcaatt
    15421 ggcaagttat gctttagcat accacttact atagacttta aattttttgc tgcgcttcct
    15481 tctcttatta aaatttttaa tcctgctctc aatttttcat atacttcatc aaaagttata
    15541 cattcatggt cattttctat tcctgcacat acatatgtct gaagtgattt accatttata
    15601 ttaggtgcat gtccatctac aactttattt ttaaaaagct ctagtttatc caagatttca
    15661 ttttcagaat ttataacatc attaaaacac attacctctc ctaaacctaa aattctagga
    15721 ttatttacat aactctccat gtcttttgca ctaaatttac caactccatt agtttcaaaa
    15781 cttgttgcag gtacggatga aggcaccata atatatacat taagtggtat atcttttgtc
    15841 gattctaata aaaaatctat tgcactaact ccttttacat tgaccaactc atgtgggtcg
    15901 gctatgatag ttgtcgtacc tgattttaat aattttttcg ataattcaat tggcattacc
    15961 attgtagatt caatatgcat atgtgcatca ataaagccag gtgatatata tttgttacta
    16021 caatcaattt ctacattgcc cttgtaatcg ccaattccaa caatagtgtc tcctactatt
    16081 gctacatctc ctgttattaa ctcttctgta aatacattta tgattgtccc attttttagt
    16141 acaatgtcag ctttttctct tccacttgaa acatcacact gtctttttaa attacccatt
    16201 tttacaactc ctcctaagtt ttttatatca atatactgac tgtattacgt attatagttt
    16261 aatttattaa tatatattat atatgagttt tgatagaaag tatcacttga aatttgttcc
    16321 ataattcctt taattaaata gagagaacat gaggaataat attatctccc atgttctcat
    16381 taatatttaa gcaataattt aattcatatt gaatttttta tttttatatt taatcttcac
    16441 tattcttata aataaattta tgtgctttat tattttttta atacttcttt attcataatt
    16501 atatttaacc taaaaatgca taaactatca tacctacaag tattgctggt atagtagtaa
    16561 taatcatagc ccataaagaa gattttttat ccattgctaa aagtgggaaa agtgcatctc
    16621 catcttgaga aatagagttg gctatcactg caccaagtgg tatagttccc ttcaaataaa
    16681 atgacataag cacaatttgt atcccacaac ctggaataag tccaagacct gcacctataa
    16741 atacacttat tacacctgtt gataatacta atttaactaa gtattcatct ccacctaaaa
    16801 ttaatattat caaatcgtaa ataagatatg caataaaaat ccatgtaatt acaaatgata
    16861 tttctccaac agaatggata agcatttctc gcaaagaaat ctttttattt tcttcctctt
    16921 tttgattaga atttttaaat acctttttaa aacaccacat ataaattatt gaaaataata
    16981 ttccaatcac tgctattact tcttccaatg aatgtaactt ctcaattata ggaagattaa
    17041 gtccagagtg agcaagtgac ataaatataa aaccgattac tataattcct atataaattt
    17101 tatatccctt tccatgtgtt attataaatg caagtctatt tgtatttcca tgagatttag
    17161 ctagagtgtc taaaattatt tcatcatgat tatgttgttc tttattttct ttagggttat
    17221 ttttacttat atcatattta tctgattttc tacggttttt taaccctaat ttttcttcta
    17281 gtttaaagta atccactaat tgtccagtta ttattcctgt aattgtacta actatagtga
    17341 caaaaaaata cattttaata tttgatgaaa taagtacaaa tgctgcatct cccatactag
    17401 ctattaaact tgcaattatt gctccaaagc ttaattttcc atttatatac aatggcatta
    17461 ttgccaaact tccgccacaa cctggtatag ctccaattaa agcacctatt aagggttgaa
    17521 attttctgtt ttttgatatg atattagtaa aatttccact agttttataa ttaatatatc
    17581 caaataacaa aataaaaaat ccaatcatag aacctacatg taaaaacgcc tcttcagaag
    17641 cggatatcaa catttccatt aatttacctc ctagactaat tttgcaacaa ttatattata
    17701 ttctattttc tattgataat caatatcaat atttcttttg aatttctaat attaaaaagt
    17761 gcgcatctta tactcttagt ttttcatctt aagatacaca ctttttgata ttaggttatc
    17821 tatttaccac atgtaatatg aaatcaaagg tggatattat gataaattat aacaatgtcc
    17881 acctattttt attacctatc aaattataaa ttttcaagga atgttacaac ttcttctatt
    17941 atccaattag gtgtagatgc ccctgctgta atacccaaat ttttatattt tttaagcgta
    18001 tttacatcca attcatcttt agtctctatt gcaaaagttg gtactaagtc ttcacaaacc
    18061 tttactaact tttgagtatt tgaactgtgt ttacctccta taactatcat acaatcaact
    18121 tctttagcta actctttagc tgattcttgt cttgtttttg tagctgaaca aattgtatca
    18181 ttaaatacta tttcttctaa tttatctgat agtttattaa ctatacttat atacaactct
    18241 ggatttattg tagtctgtgc aacaagacaa tatcttttgg agttatcaaa ttccatattt
    18301 tccatttgtt ctaaggtctt tattattata gctgaatttt cacaccatcc atttatacct
    18361 ataacttctg gatgcttcat atctccaata actattattt catagccttt attatgatat
    18421 tcacttacta ctgtatgtat tttctttaca aatggacatg tagtatccac aatttttaat
    18481 tttttatctt tggcttcctt atatatattt tcaggaactc catgagacct tattatcata
    18541 ttttcatgat ttggtattgt atcaatttca ttcacagtct taagaccttt ttcttcatat
    18601 tttgctactg cttgcttgtt atgaataaga ggacctaaag catatatgcc agaatcattt
    18661 ttttcaactt catcccatgc cattttcatt gctcttttta ctccaaaaca aaagccagca
    18721 ttttttgcta tttttatgtt cataacttta cccccttatt ttgaatattt aaagtaattt
    18781 tgttttatca cttctaaaat ctcttgtgat atcctttcat ggtcatcatt actcaatttt
    18841 tccttaaaat gttcttcaaa tgatattggt tctccaatat atacaattac tttactaaaa
    18901 agtttatatt tagatattat tgatactgga acaactttag atttactttt tattgtaaat
    18961 acagaaagac ctgccttagc cttaccaaaa gaatctcctt ttattctagt accctctgga
    19021 aatattccta atacataacc gtctctaact gaccttaaaa tagtttttat tgtagataca
    19081 tctggttttt ctctatttat aggcataaca tgcaattttt ttaatataaa tcccaatggt
    19141 tttactttaa acaattcttt ctttgcaatt gcagctattt ctctattttc tatggcagca
    19201 gccaagaata tagggtcaag attcgattta tgatttgatg ctattactat atttcctcta
    19261 tctggaatat tctctgcacc tataacttca tatttaaaaa atattttaga aaaaccctta
    19321 aaaatattta ttacaaatct ataaaaattc acgtttatct cccttcaaca ctacatttat
    19381 tttcattatg atgcataaaa aactaaagat ttattttaga tacaacttct tctacaactt
    19441 cttctatagt ttttcctgta gtatctattt ctagtgcatc attagctttt accaatggtg
    19501 caaattctct atttgaatca atttcatctc gtcttattat atcctcaatt acttcttcta
    19561 aagtagtatt atatccttta ttacaaagtt ctttatagcg tctatttcct ctttcttctg
    19621 gagtagctac taaaaagaat ttgtaatctg catctgggaa aacatatgaa cctatatctc
    19681 taccatctag tattactgaa tttttcattc ctatttttcg ttgaacttca accatcaact
    19741 gtcttacttc ttttattttt gctacattag aaacattatt gcttacttct attgttctta
    19801 tttcttcatt tataacttct ttgtccaaat atatattgtt atctttaaaa tctatatccg
    19861 aattttcagc tatttgaata acttcttttt cattattgac atcaatacca ctttttaaac
    19921 acttataagt aactgctctg tacatagcac ctgtatctat ataatttata tttagctttt
    19981 ttgctactat cttggctatt gtactttttc cagctcctgc tggaccatct acagctataa
    20041 ctaaattacc catatataca cctctattta ttcatattta attattaaaa aattatattt
    20101 atacacttat acccgcaaga tatccagttg aaaatgctat ttgtaaatta tatccaccag
    20161 tttctgcatc tatatcaatt acttcacctg taaaaaataa gttcttaact aatttagatt
    20221 ccatagtaga agaattaatt tctttaactg aaataccacc agaagtaacc ataccagtgt
    20281 ttatacctgt agtcccatta caagttagct tcatattttt tatatactca ataattctaa
    20341 tttcatctgc cttagacaaa tcacttgcct ttttatcaac taaagataat agagaaaaaa
    20401 tctcttttaa gaaattttga ggtagtattt gcttaagatt atttagaaca tttttatttg
    20461 gaaatgctct gattatacta gagatttcat cagtacttaa atttggtaag aaatctatat
    20521 ttaattctac cttttccttt tcaataatct tgttaatata agaagacatt ataagaacac
    20581 aaggtcctgt tatcccaaaa tgggcaaata gcatgtctcc agattttgat attttccttt
    20641 tcttaatctt acaagatatt tccacattct tcatggatat tccttgtaaa tctttaatcc
    20701 atttttcctc tattgtaagt ggaactaaag caggatatag tttgtttaat gtatgtccat
    20761 gctttttaag aatcttatac attgaaccat ctgaacccgt ctttgaataa ctcactcctc
    20821 cagttgatat aataaccttg tctcctagta ttttatctcc attatctaaa ataatccctt
    20881 ttattagata ctttgacttg tccttattag aatcatcttt tagagcaatt tcttcagtta
    20941 taatatctat aactttctta ttatacatta tctttacatt gtgattgagt aaatctttat
    21001 ttaatacttc tattacttct atagatttat catttttagt gtatactttg tggtcatttt
    21061 cttcttcaat cttatattcc aaatttctac tttcaaaata cgaaataagg tctttatttg
    21121 taaatgtata aaaactacta tagagaaatt ttttattact gacaacttta tcaaaaaaat
    21181 cttctatctc tctattattg gtaaaattgc atctaccgcc accagttgcc ctaagttttc
    21241 ttcctaattc tccatttctc tcaactaata ttacttcatt attgtttttt gaagctgaca
    21301 aagctgccat cattccagaa gctcctccac caactactat aactttactc ataaatttct
    21361 ccttataatt gattcctact ccagtgcaga attatttatc tttacatgat tttttatatg
    21421 acttgtatca ttcattttta ctataacagg cccataatct ttaaattcta ctatatttaa
    21481 agcagtatta tcttgcttaa ttctatatat attttccata cctgattcta aaaatgacaa
    21541 caacataacc ctcaatgtaa ttgaatgtgt aacaagaatt atatttttat tatcatattt
    21601 ttcatttagt tcctttataa aagcatctac tctttccttt ataatcttaa gagtttcgcc
    21661 ttctggtata tttactaagt gtggttcatt tctccatgtt gcatatatat ctgaataatc
    21721 tttttgaatt tcttttatta aaaggccttc ccaaactccg aatcccattt ctctaagtgc
    21781 ctcagttttt tgaacttcaa tattgagctt atctcctaaa atttgagctg tttgcattgc
    21841 tctcccaagg tcgctactaa aaatataatc aattgaatac ttgccaatat cttcagaaag
    21901 ttcttttgcc tgttctatac cttgaggcgt aaggtcagaa ttaccatgac cttgtgtttt
    21961 tcccaatata ttccaatccg tttgtccatg tcttactata taaaaagtat ttcccatatg
    22021 tcctcctaat atatgcctat tttttattaa atataaatta tcacttctca aaacttaatt
    22081 atatcatata atactttact ttgtttctat tttataatct aatctaacat attatttaca
    22141 ataaatctca tttgtatttc tgcaaattat tgtactgtta aagaattttt ctaaatttat
    22201 aaacatattt ctcttatatt atttatatac ttcttcaaat ctgtattctt gaattgaatt
    22261 gacatactta tctgtatcta ctttttctaa aaacatatcc aaaggtcttg caaatattcc
    22321 tgtatcatca tataaggtct tgtataaaac taaatcatta gtatcttctt cttttttatg
    22381 ataaaagttt ccatctaaat ccctataaac atagatatct tcttctcttt cagtatgtct
    22441 aataactaat ttatatttat ttctgttttg gattaaattt tctttaccat atatttcaca
    22501 tatgttttct atatcatcaa tagccttgct aagacccatt gtagcataat atttcccttt
    22561 gaaatgttta tagatacaag gatattttaa ctttctttca ctgataccta agttattctt
    22621 taattcaagc attttattat aatctcccat atctcgtccc tttcttttaa aaagtatttt
    22681 attctgaaat aagttacttg ttttatattt tatcataatt taaaatttat taataataca
    22741 atcaataaat aaagaggctg tataaaatac agcctcccaa tattaatctt cataatcata
    22801 taatttttct aatttctttt ttaatttctt ttttctttct gaacctaaaa ttcttataaa
    22861 attattattc acaaaaatac aattattgct gagataaaat tcacacttgt ctagatttac
    22921 aatataacta ttactgcatt tgaaaaaatt ttcttctttc aatagttttc ttatacaagg
    22981 agcattttct ccaacttcat aattaccact ttcagtataa atctttactt catactcttg
    23041 cttttctatg tacaaaactt ctttactatt aattgaaaaa cgtttatcct cgtattcaac
    23101 attaataaaa ttttcctctt tcatttccat ccctccaagt acttttatta atttaatttt
    23161 agtgtttttt atcaatataa tctatcggaa atatatgtaa gactgattta cttaagatat
    23221 ttaaataatt attattaata ttaatgtata agtttatttt ttaacattaa tttttaaagt
    23281 taaaaagact ttttttaatt atatccgtat atataagtat atctatcttt aataaaataa
    23341 cttttataga aattcatgta aaattataca taaaaaagta attattaact tttaatttaa
    23401 atgaatcgta ttaaaattta tcttattttc ctaattttaa
//
LOCUS       DAGSRM010000055        23393 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.055, whole genome shotgun sequence.
ACCESSION   DAGSRM010000055 DAGSRM010000000
VERSION     DAGSRM010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23393
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.055"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            362..751
                     /locus_tag="MJE33_002324"
     CDS             362..751
                     /locus_tag="MJE33_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBY9648256.1"
                     /translation="MKINKLPHAELKLMKYIWGVDDVLASRDIIEDMKLKYDWKKSTT
                     LTFLKNLVDKGFLTTDKVDRCTHYTIAIKEKDYLKVETKSFFSFMHNNSFKSFISALH
                     DDEVLDSKSLDKLEEYFKNLKEEDIDD"
     gene            1071..1580
                     /gene="vanZ1"
                     /locus_tag="MJE33_002325"
     CDS             1071..1580
                     /gene="vanZ1"
                     /locus_tag="MJE33_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ1"
                     /protein_id="HBY9648257.1"
                     /translation="MKSRKHNITKGLFIVYIIILTWIILFKLQFDISSLETMNLRSIN
                     LIPFAGSLIINNRVDISEIILNVAIFVPFGIYVCMLKEEWSFIKKVIPIFITSLVFET
                     LQYIFALGASDITDLIGNTLGGIIGIAVFMLLSKIFKNNTIKIINVLALIVTIIVVLF
                     LGLVIFANL"
     gene            complement(1682..1876)
                     /locus_tag="MJE33_002326"
     CDS             complement(1682..1876)
                     /locus_tag="MJE33_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HBY9648258.1"
                     /translation="MSVIVMCSRCGKEIDKYRNKYITYYEKNTNAEIKVCINCALDLL
                     EKTRENQELNIQEFQDTIIL"
     gene            complement(2392..2784)
                     /locus_tag="MJE33_002327"
     CDS             complement(2392..2784)
                     /locus_tag="MJE33_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBY9648259.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGTDKEGNEINTTLYM"
     gene            3351..3713
                     /locus_tag="MJE33_002328"
     CDS             3351..3713
                     /locus_tag="MJE33_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648260.1"
                     /translation="MKIRKSGFISIECIISIAILYVAVYLVSTSLYNCYSFISRNISD
                     REMLSTAKKYIEDEKYRIQNSKYELIEDKIEKNYINGYEINSKIEQILDYYQCYEINI
                     EIKNEFKKLRFNSYVTRK"
     gene            3697..4422
                     /locus_tag="MJE33_002329"
     CDS             3697..4422
                     /locus_tag="MJE33_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648261.1"
                     /translation="MLLENKGSILIITLIIFSIISTVCIMCIGLIYTHNNMFELGYKD
                     IQLKEISLSGIEISVSNVLSCVEEAIDVSNNEDEFREYFLGNNMIECIKKIKDTSYSS
                     LEELSLKVENNTIYDRGDFFEFDIESNVREKEFLKAIKVRVKIKNPWIGIDIEDYMDK
                     SNNNKIDDKTFNNYKASENNEILNQFKNESENIRNQEVGGFTEEDEEDEEYEEELKQN
                     IIENIKKNFDEKNLVQVYKYRGV"
     gene            4442..4861
                     /locus_tag="MJE33_002330"
     CDS             4442..4861
                     /locus_tag="MJE33_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648262.1"
                     /translation="MTVIELIVSISIILLIVTLSFPKDNLENHKINLFARQLCSDIRY
                     VRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIMLPKNAKIARSIEIIKFKSDG
                     SPQKASTIEIYNNRLRKTITITPVSGRVLLKEGKYET"
     gene            4851..5375
                     /locus_tag="MJE33_002331"
     CDS             4851..5375
                     /locus_tag="MJE33_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648263.1"
                     /translation="MKHKYGYLLLESVVSLSSMVIIILVLYSIFLSTINLKLKVEDKI
                     ELQQQSLEIIKSMEGIISNSMGIMNVSNYEETFKKTTSIKCRYLDENNNEEGISNKEI
                     ILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVSITNNGQYVNIKLKLSKRSQKYE
                     TDFKIKVWNFSESI"
     gene            5687..6244
                     /gene="efp"
                     /locus_tag="MJE33_002332"
     CDS             5687..6244
                     /gene="efp"
                     /locus_tag="MJE33_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBY9648264.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYEQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            6336..6791
                     /locus_tag="MJE33_002333"
     CDS             6336..6791
                     /locus_tag="MJE33_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBY9648265.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFADAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCSSCDDRE"
     gene            6954..7664
                     /locus_tag="MJE33_002334"
     CDS             6954..7664
                     /locus_tag="MJE33_002334"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBY9648266.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGI
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINDDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            7718..8788
                     /locus_tag="MJE33_002335"
     CDS             7718..8788
                     /locus_tag="MJE33_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBY9648267.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPADTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYNPFTLEESVN
                     IVKKVLVLFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADMIKDYLEFV
                     ILQNKNIKQMLVKSNKKNISKIIGNKKTNVKYMEEKFGVLLKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            8816..12370
                     /gene="smc"
                     /locus_tag="MJE33_002336"
     CDS             8816..12370
                     /gene="smc"
                     /locus_tag="MJE33_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBY9648268.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDVYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKTLEVNSFIREIEGIEKELSEVNEHRKVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEIKDIKEKLNE
                     NKQYIKELESNKLSGSEELSTLQENIKVLEGSKDKQKIKLESLNNEIELLKESIIDIL
                     NKKQEFSNKLSTLNANKENMNIRDENINSEITELNKNIEIKSSELDTINKEFNMQNEN
                     LKNVNNRHKELSINLQDSISEHNKLEDEIQKSKYNLNGYNSKLNVYIDMENHYEGFNR
                     GVKEVLKNKNLKGVHGALGQIINVPEKYEKSIEAALGAYMQNIITDNEFSAKFAINYL
                     KQNNLGRVTFLPLNIIKSNKISLGNLKANTKFIGIASDLITFDEKYRNIIENILGRTI
                     LINNIDEGIKFAKETGHRFKIVTLDGEILNPGGSLTGGSLKTNGNILSRKRYINEYTE
                     KISNIKNEISHLELKRESLDKDVKNIKNEIDSHESKIKDLEKSIIIKSTSIKNVESEI
                     ESLKGSITKLENEKNDLNSNLNYTLEKSDDVRKDMEELDDLYNKNKEKIDALNEEIKR
                     YNDLYDKEKSEFDELNLSLVKKTEVYNSIVRDIKRISGENCELEEKNKQLEESLNYEE
                     HEIIKLQDSILTEEKEKENLTKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQ
                     HIELKESLFKVGGRLERLKTSQDTYINKLFEQYDMTLVQALEIKDEDLDIDRKFLESL
                     KREIRSLGNINIDSIKEYEEIKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFE
                     IKFEEISKNFKYVYKRLFGGGCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGG
                     EKALTAISILFAILITKPTPFCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHR
                     RGTMEAADYIYGVTMQEKAISKVISLKLKEAQEITDII"
     gene            12397..13677
                     /gene="ftsY"
                     /locus_tag="MJE33_002337"
     CDS             12397..13677
                     /gene="ftsY"
                     /locus_tag="MJE33_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBY9648269.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDAEEEIEVEDSVDNLENLEETIFSG
                     LEEEVIDKVEDIEEKNEESVNNEGTEEIENFEKIEVEMDSNGKEVENISNNDSQEEII
                     EELENYDEIESKEIEDKEDKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELL
                     EDLEEILITADVGVNTTMDIIERLRDKIKQKGITEPIKVREELKSIVEDILTNENSTL
                     DIEPAPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWAN
                     RTNVDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKI
                     VDREFPEAKREVLLVVDATTGQNAVVQAKTFKEVADITGIVLTKLDGTAKGGVVLAVK
                     SEVDVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            13780..14148
                     /locus_tag="MJE33_002338"
     CDS             13780..14148
                     /locus_tag="MJE33_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBY9648270.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNRD
                     CANLIPKLENIEDVCREMIK"
     gene            14145..15497
                     /gene="ffh"
                     /locus_tag="MJE33_002339"
     CDS             14145..15497
                     /gene="ffh"
                     /locus_tag="MJE33_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBY9648271.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEVDVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPINIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMDELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIAKGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            15535..15807
                     /gene="rpsP"
                     /locus_tag="MJE33_002340"
     CDS             15535..15807
                     /gene="rpsP"
                     /locus_tag="MJE33_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBY9648272.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            15872..16099
                     /locus_tag="MJE33_002341"
     CDS             15872..16099
                     /locus_tag="MJE33_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBY9648273.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIVLKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            16207..16722
                     /gene="rimM"
                     /locus_tag="MJE33_002342"
     CDS             16207..16722
                     /gene="rimM"
                     /locus_tag="MJE33_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HBY9648274.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNNKLAVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVAREDSRELEEGEFFIA
                     DMIGMEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            16731..17426
                     /gene="trmD"
                     /locus_tag="MJE33_002343"
     CDS             16731..17426
                     /gene="trmD"
                     /locus_tag="MJE33_002343"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBY9648275.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVHIYNIRDFSNN
                     KHKKVDDYPFGGGAGMVMTPQPIYDTYKHIITTHNINNPSVIYLTPKGKVYNQSMAKQ
                     MSLKEDIILLCGHYEGIDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            17611..17961
                     /gene="rplS"
                     /locus_tag="MJE33_002344"
     CDS             17611..17961
                     /gene="rplS"
                     /locus_tag="MJE33_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBY9648276.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
     gene            18282..19178
                     /gene="ylqF"
                     /locus_tag="MJE33_002345"
     CDS             18282..19178
                     /gene="ylqF"
                     /locus_tag="MJE33_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBY9648277.1"
                     /translation="MSNEYEEYLMNDNLHINWYPGHMKKTKELVKNNLKLIDVVIELL
                     DARIPFSSKNPDIDRLVGDKPRVVVLNKSDMADRDKLNQWIEYYKKINIKAIPVDTIK
                     GVGINKIIEECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQ
                     TGDKPGVTKGKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETL
                     ALRLIEKLMKIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIATTV
                     LNEFRDGKIGNITLEVPENVKR"
     gene            19567..20859
                     /gene="brnQ"
                     /locus_tag="MJE33_002346"
     CDS             19567..20859
                     /gene="brnQ"
                     /locus_tag="MJE33_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBY9648278.1"
                     /translation="MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFAISYVLTIRPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLLVSILTVISSYGVAVPVV
                     NSLPLNSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
     gene            21978..23270
                     /gene="brnQ"
                     /locus_tag="MJE33_002347"
     CDS             21978..23270
                     /gene="brnQ"
                     /locus_tag="MJE33_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBY9648279.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLV
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKGLSIALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
                     VLIVKGIVSPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     EKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIQFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSFGIK
                     IPLVNSLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
BASE COUNT         9789 a         2208 c         4142 g         7254 t
ORIGIN      
        1 ttaaatatta ataaaaaata tagtaaaact taataataca tataataaaa cttaatataa
       61 attaagtata tgaaataaat atcctgtatt taagcttata gattataatg aaatataata
      121 catgttgcaa taataaaacg aactaaaatt atcacactaa agcataaaac atactctaat
      181 catgttagat aaatttttga atatataatc caaaagtact ctctaaagta gagtacttct
      241 tttttattaa ttatttttat atagaataaa ttggaattta tatttattcc aatctaattg
      301 attacataca cttattgtta taaagtttag ttatacataa attggtaaga ggtgtaaact
      361 aatgaagatt aataaattac cacatgcaga actaaaacta atgaagtata tttggggagt
      421 agatgatgta ttagcctcaa gagatatcat agaagacatg aagttgaaat acgattggaa
      481 aaagagcaca acattgacat ttttaaaaaa tttagtagat aagggctttt taactactga
      541 taaggtagat agatgtacgc attatactat agctataaaa gagaaagatt acttaaaagt
      601 tgagacaaag agttttttta gttttatgca taacaattca tttaagagtt ttatttcagc
      661 actacatgat gatgaagttt tagatagtaa gagcttggac aaattagaag aatactttaa
      721 aaatttgaaa gaagaagata ttgatgacta ataaagctat cagtatcttt ttagtttgag
      781 acaattgaga gtagcgtttg gatttaatgt tagttttatg tgatggtaga acattatata
      841 aagaaaagtg attatttcct tagtgtttga tagtaaaaaa tatagagaat aataccaatt
      901 aaaatttaaa gatatatgaa atactacaat agcttaaatc atgtaaatat gataaagata
      961 ttgaaattat aaaaggaata taattttaga agaaatagtt gacagataaa ttaccttact
     1021 atattattgt attaataaaa taatacaata atatagtaag gaggtaataa atgaaatcta
     1081 gaaaacataa tattacaaaa ggattattta tagtttacat aattatttta acatggataa
     1141 tactatttaa gttgcaattt gatatctcat ctcttgaaac catgaatctt agaagcataa
     1201 acttaatacc ttttgctggg tcacttataa ttaataatag agtagatata tcagagatta
     1261 tattaaatgt ggctatattt gtaccatttg ggatatatgt gtgtatgcta aaagaagaat
     1321 ggagctttat aaaaaaagtt atacctattt ttataactag tttggtattt gaaacacttc
     1381 aatatatatt tgcactaggt gcaagtgata taacggattt gataggaaac acactaggag
     1441 gaatcatagg aatagctgtt tttatgctat tatcgaaaat atttaagaat aataccataa
     1501 aaattatcaa tgtattggca ttgattgtga caattattgt tgtattattt ttaggattag
     1561 ttatttttgc aaacttatag aaataagtat atatgtttag cttaaaatag aacgtaagat
     1621 acagttacat ataaaattat ttacacataa aaagcaccta tacaggtgct ttcctaaatt
     1681 attaaagaat tattgtatct tgaaactctt gtatgttcaa ttcttgattt tctcttgttt
     1741 tttctaataa atctaacgca caatttatac aaactttaat ttctgcattg gtatttttct
     1801 cataataagt tatatattta tttctatatt tatctatttc ttttccacaa cgactacaca
     1861 ttacaattac gctcatatct tagttcctcc atctctaatt attcttaata tattcaatag
     1921 tatcactttt ctgaaaataa tgattgttaa aactgtatca aatttagatt tactttttat
     1981 tataatgcat tttaataaaa aaataaaggt tatgggtaaa atatttatag aaaaaagtat
     2041 attattgaat agattataaa attttattat tttttagcag tgttaaatac acattaatat
     2101 ctataaagtg acaaaataaa aaatactatt taaatagata ttaaaaaatt gataccaagt
     2161 atttatttgc tatatgaata acaatttatt taggaacttt actttaacac tgtataataa
     2221 taaagtaatt gctaaaaacg tttgatgatt tggatgtatt tttagaaatt agaaaaatat
     2281 atacttactg actaatgata atcttatatg tattttagtt tgagtgattt aagaaaagtg
     2341 ttttgaatgg ggattatttt atagagtaaa taaatatata atagtagaaa attacatata
     2401 aagagtagtg tttatttcat taccttcttt atctgttccg atagggtctt gtagagatac
     2461 ttgagtttta ttttttttgt tagatctaat attcattaaa atctcatttt caatacatct
     2521 agaagcatat gttgctaatc gagttccttt atctatgtta taagtttcta tagctttaat
     2581 taatcctatt gtacctatgg atattaaatc atcctgatct tccgttgaat tattatattt
     2641 ctttgctata taagctacta atctcatgtt tctttcaatt aatgtatctt ttgcactttt
     2701 atcgttttct attttaaatt tagttaaata ctcaatctct tcttcagggg ttaagggttt
     2761 ttcaaaagat ttaagagctg ccaataggtc accccctttt caacggtcac tttaaattat
     2821 atgaaggaga cttatccaag ttgactgtta tctaataaaa taatatgcat atttttagaa
     2881 tttaaaacat atatttgcaa acttttgaat ggaatatgac ataaagagtg ggaatttaaa
     2941 tttaagtttt tgttgtatat atatgaaata aggcgaagaa attattgatt tataaagtat
     3001 aaattggaat tgaggtgtca ataaatataa aaggaaatag tatttatata tgttttggtg
     3061 ttgaagtaaa agattaatgc aaagcattag agtctataaa aatatgaata taggaggtgt
     3121 aattttacaa tacttaaaaa agtaaatgta attaattgtt tgtataaagt tgattacaag
     3181 caaaagtatc agtcatagtt aatactgtta agggtgaagg tggtgtgtta atttatatat
     3241 gtatgggaag aaattttgat tacaatgaaa aaacttacaa aagatattta tgaaaaaact
     3301 tatagttaaa tgtttatata taaaatatta tttaggggtt gtttttatta ttgaaaataa
     3361 gaaagagtgg ttttatatca attgaatgta taataagtat tgctatatta tatgtggctg
     3421 tttatttagt ttctacatca ttgtataatt gttatagttt tatcagtaga aatatatctg
     3481 acagagaaat gttaagtaca gcaaaaaaat atatagaaga tgagaagtat agaatacaaa
     3541 atagtaagta tgagttaatt gaagataaga tagaaaaaaa ttacataaat ggatatgaaa
     3601 ttaacagtaa aatagagcaa attttagatt attatcaatg ctatgaaata aatatagaga
     3661 taaaaaatga atttaaaaaa ctgaggttta atagctatgt tactagaaaa taaaggctct
     3721 atattaataa taacattgat aatattttct attataagta ctgtgtgcat tatgtgtata
     3781 ggtttaattt atacacataa taatatgttt gagttaggat acaaggacat tcaactaaaa
     3841 gaaatttctt taagtggaat agaaatatca gtaagtaatg tactttcttg tgttgaggaa
     3901 gctattgatg tatctaataa tgaagacgaa tttagagaat atttcttagg aaataatatg
     3961 attgaatgta taaaaaaaat aaaagataca tcttatagta gtttagaaga actttcttta
     4021 aaagtagaaa ataatactat ttatgacaga ggagattttt ttgaatttga tatagaaagt
     4081 aatgttagag aaaaagaatt tttaaaagca ataaaagtaa gagtgaaaat caaaaatcca
     4141 tggataggaa ttgacattga agattatatg gataaatcta ataacaataa aattgatgac
     4201 aaaacattca ataattataa agcatctgaa aataatgaaa ttctaaatca atttaaaaat
     4261 gaaagtgaaa atataaggaa tcaagaggtt ggtggattta cagaagaaga tgaagaagat
     4321 gaagaatatg aagaagaact taaacaaaac ataattgaaa atataaagaa gaattttgat
     4381 gaaaaaaacc tagtacaggt atataagtat aggggtgtat aactttgaaa aaagaaggaa
     4441 tgtgactgta atagaattaa tagtatctat cagtataata ctcttgatag ttacattatc
     4501 ttttccaaaa gataatcttg aaaatcataa aataaattta tttgcacgtc aactttgttc
     4561 agatataagg tatgttaggc gaattaatat gtttagcaac tacaatacat atatatatta
     4621 tataaatcaa ggtaaacacg aaggttatgt attgagaaaa gatggtaaaa acattaaatc
     4681 aataatgcta cctaaaaatg caaaaatagc taggagtatt gaaataataa agtttaaatc
     4741 tgatgggtcg cctcaaaagg cgtctacaat agagatatat aataatagat taagaaaaac
     4801 tataacaata acacctgtaa gtggaagagt acttctgaaa gagggaaaat atgaaacata
     4861 aatatggtta cttgcttttg gaaagtgtcg tatcattgtc ttcaatggtg ataattatac
     4921 tagttttata ttctatattt ttgtctacaa taaacttaaa gttaaaagta gaagataaga
     4981 tagaactaca acaacaaagt cttgaaatca taaaatctat ggaaggaatt attagcaatt
     5041 ctatgggaat aatgaatgta tctaattatg aagaaacatt taaaaagaca acatctataa
     5101 aatgtagata tttagatgaa aataataatg aagaaggcat aagtaataaa gaaattatat
     5161 tgaatgaaag acgaaataag ttatttgtaa attctttaaa tggcgaaagt agtcaagcag
     5221 gtggttatga aattggagat tatgtagatg agatgtatgt atcaattact aataatggtc
     5281 aatatgttaa tattaaattg aaattatcaa aacgtagtca aaaatatgag actgatttca
     5341 aaataaaagt atggaatttt agtgaaagta tatagaaata cataaattta agagaaagaa
     5401 gtgacatcaa taagtcatat aaaatataat tattgagaac tatataacta aataatatca
     5461 atatgtaaag tgtatagatt aagaatatag aggataataa taaaaatata gaataagtaa
     5521 tttgccaaat acttgtttat attgtataac ttatgatatt atgatattag taaaaatttg
     5581 taatcctgac ttgacagaat tcaagtaaga gatataattt taaaggcata gtaatgtaaa
     5641 atttactaaa aaataagaaa tttattttaa tatggaggaa caaaaaatgg tatcagcagg
     5701 tgattttaga aaaggtgtta catttgaaaa agatggacaa ccatgtttag tagttgattt
     5761 tcaacacgtt aagccaggta aaggagctgc tttcgttaga actaaataca aaaacttaaa
     5821 aacaggagct ataagagaag aaagtttcaa tccaagtgaa aaattcccta aagctgttat
     5881 agatacaaga caaatgcaat atctatacaa tgatggtgag ttatattatt ttatggatca
     5941 agaaaatttt gaacaaatac cattaaacta tgagcaagtt gaagatgcaa ttaagttctt
     6001 aaaagaaaat gaagttgcta caataagatt ttaccaagga caaccattcc aagtggaagc
     6061 gccaaacttt gcagagctag aagttacaga tacagagcca ggtataaaag gtgatacagc
     6121 aagtaatgta actaaagcag ctacagttga aactggagca gttgttcaag ttccattatt
     6181 tataaatact ggagataaag taaagataga tactagaaca ggtgaatatt tatcaagagt
     6241 ataaaaattt atgtatataa aagcttatat gtcttaataa tataagatat atgagctttt
     6301 agtttatata taaaaaagaa agcgaggggt gttacatgaa acatttatat gaagaagttg
     6361 catacttaaa aggcctagca gaaggactag aaataagtgc tgaaagtaaa gaagggaaaa
     6421 tgatacataa aatagttgat gcattagaag tatttgcaga tgctattgta acattggatg
     6481 aagaacaaga agaacttcaa gattttgttg aatctataga tgaagattta gcagatttag
     6541 aagaagatat tgctgacatg gaagaagatc tttatgaaga agatgacgat gaggatgatg
     6601 aggacttcag ctatatagaa atggaatgcc caaattgtgg agagttagta gaaatagacg
     6661 aagatttgtt atatgatgat gaagtagatg ttgtttgtcc tgattgtaag gctgtgatat
     6721 tatcatcaga agatgattgt gatgatgatt gtacatgtgg tggttgttca agttgtgatg
     6781 atagagagta aaaattcata agaattaaaa ctgcctatga tgcctatgag atattttgct
     6841 tcataggtag ttttattttt atgaggaagg gtattatgta ttttggtttt gcaatacttg
     6901 gacaaaaata gtacactaga atttatagaa taggattttg gagggatgta aatatgaaaa
     6961 taagtaaaaa attactagat aatatacaaa gatttgaaaa tgttataaat tataaattta
     7021 aaaataaaga atatatacta gaagctctta ctcatagttc atattctaat gaaaataaaa
     7081 aatataattt taacgaaaga ttggaatttt taggggattc ggttttaggc atagtgataa
     7141 gtgattattt atttaatgaa gaagctaatc tgcccgaagg ggaactaact aaacttagag
     7201 caaatatagt ttgtgaagat tcattaagtg aagtagctaa tgatataaat ttaggtattc
     7261 acatgttatt aggtagagga gaagaagcaa caggaggaag acataggact tcaatattag
     7321 cagacgcatt tgaagctgta atagctgcta tatatcttga tggaggtttt gaaagtgcaa
     7381 gacagtttat acttcatcat atggaaaata taatttatga ttcaagaaaa ggaaatatat
     7441 ttagagacta caaaacgcat cttcaagagg tgttacaagg gaatggagaa aataatatct
     7501 ggtacaggct tatagaagaa aaaggaccag accataataa aagatttgta atggaagttg
     7561 gaattaatga cgatgtactt ggtataggag aaggcaagag taaaaaagaa gctgaacagt
     7621 tagctgctaa aatagcttta aagaaaaaat tatgggaaaa ataaatataa tatgtaaaaa
     7681 tataaaaaga aatatttata ttagaaggat gttgtagatg aaaaagagaa ttatacctat
     7741 atttgttcct cacaaaggat gtcctcatga ttgcatattt tgtaatcaaa aaaaaataac
     7801 aggtgtgagt actgatgtta ctagtgaaga tgcaagaaat ataattgagg aatgtttaga
     7861 gactatagat aaagatgcag atgtagaaat agcatttttt ggtggtagct ttactgctat
     7921 agatattgat atacaaaaaa gtttattatc agttgctaaa gaatatgtag aaaaaggtct
     7981 tgtaaaagat attagaatgt ctacaagacc agattgtatt gatgaagata tattaagtat
     8041 gttaaaagaa tataagacaa gtataattga actaggagta caatccttag atgaaaaagt
     8101 attattagat agtataagag gtcatcaatc tgaaattgta tacaagagtt caaagatgat
     8161 aaaaaatagt ggaatcaaac ttggattaca gatgatgatt ggtcttccag ctgacacaga
     8221 agaaaagtgt atatttacag caaaaaaatt tgtagagcta aaaccagatt gtgttagagt
     8281 ctatccaact ttggtagtaa aagatacagg tcttgaaaag ttaatggaac aaaataaata
     8341 taatccattt actcttgaag agagtgtaaa tatagtaaaa aaagttcttg tactgtttta
     8401 tgttaataat atcaatgtaa taagagttgg gcttcaagct actgatgata tacaaattgg
     8461 aaaagctgtt ttagcaggac catatcatcc tgcatttaga gagttggtag aagcagatat
     8521 gataaaagat tatttagaat ttgttatttt gcaaaataaa aatataaagc aaatgttggt
     8581 caaatctaat aaaaagaata tttctaaaat cataggaaat aaaaaaacaa atgttaaata
     8641 tatggaagaa aaatttggtg tgcttttaaa aacacaagaa tcagatttag atataaatca
     8701 attagaaatt gtgttagatg ggaaaagttt aattagtgct aatatgagag acattcatag
     8761 aaaattgtat gacatttatg acctttagtt ttaataatga agggagagaa aagatttgta
     8821 tttaaaaaga ctagagttaa agggatttaa atcctttcca gtaaaaacag atattatttt
     8881 taaagaagga ataacagcca tagtaggacc aaatgggagt ggaaaaagta atatatctga
     8941 tgctgtaaga tgggtattag gagaacagag tattaagagt cttagaggtg ataaactcga
     9001 agatgtaata tttgcaggta cagatacaaa aaaacctatg aattattgtg aagtggctct
     9061 tactattgat aattctgaaa atcaattgga attagatttt acagaagtaa ctataagaag
     9121 aagggcttat agaaatggtg aaagtgaatt ttttctaaat aataaaagtt gtagattaaa
     9181 agatataaaa gaagtctttt tagatactgg tattggaaaa gatggttatt caatcataga
     9241 acaaggaaaa gtagatgaaa tattaagtaa taatccttta agcagaagaa aagtttttga
     9301 tgaagcttgt ggcatatcaa aatatagata taaaaaacag gaagcagaaa ggaatttaag
     9361 taatacaaag gaaaatttag aaagaataga tgatgtatat atagaaattg aaaatcaatt
     9421 aaagcctctt tttaatcaac agacaaaggc aaaaaagtac ttggaaataa gtgaaaaatt
     9481 aaagacactt gaagtaaata gctttataag agaaattgaa ggaatagaaa aagaattaag
     9541 tgaggttaat gaacatcgta aggttattga aaaggagctc aatgaaaaag aagaacaaaa
     9601 aaatgttgta gagaaaaagc aagaagatat taataaagag gtagaggtat tacaagatgt
     9661 aattgaaaaa tctgtagatt atataaattc aattaaagga gttatatcaa aaaaagagtc
     9721 ccaaataaat ttaattaagg aaagaataag aaattttacg aatgaaatta gtagaaaaaa
     9781 tttagaaata aaggatataa aagagaagct aaatgaaaat aagcaataca taaaagaatt
     9841 agaaagcaat aaattatcag gtagtgaaga actatctacc ttgcaggaaa atataaaagt
     9901 attggaagga agtaaagata aacaaaaaat taagttagaa tccctaaata atgagataga
     9961 attattgaaa gaaagtataa ttgatatatt aaataagaaa caagagttta gtaataagct
    10021 gtctacttta aacgcaaata aagaaaatat gaatatcaga gatgaaaata taaattcaga
    10081 gataacagag ttaaacaaaa atattgaaat aaaatcatct gaattagata ctataaataa
    10141 agaatttaat atgcaaaatg aaaatttaaa gaatgtaaat aatagacata aagaattatc
    10201 aattaatttg caggattcaa ttagtgaaca caataaacta gaagatgaaa tacagaaaag
    10261 taaatataat ttaaatggat ataattcaaa gttaaatgtc tatatagata tggaaaatca
    10321 ttatgaagga tttaatagag gagtaaaaga agtactaaaa aataaaaatt taaaaggagt
    10381 tcatggagca ttaggacaga ttataaatgt acctgaaaag tatgagaaat ctatagaagc
    10441 agctcttggt gcttatatgc aaaatataat aactgataat gagtttagtg ctaagtttgc
    10501 aataaactat ctaaaacaga ataatttagg tagagtaaca tttcttccat taaatataat
    10561 taaatcaaat aaaataagtc taggaaattt aaaagcaaat actaagttca taggaatagc
    10621 aagtgattta attacatttg atgaaaagta tagaaatata attgaaaata tacttggaag
    10681 aacaatactt ataaataata tagatgaggg cataaaattt gcaaaagaaa ctggtcatag
    10741 gtttaaaata gtaactttag atggtgaaat cttaaatcca ggaggttctt tgactggggg
    10801 aagtctaaaa actaatggga atatactatc gagaaaaaga tacattaatg aatatacaga
    10861 aaagataagt aatatcaaga atgaaatttc acatctagaa ttaaaaagag aatctttaga
    10921 caaagatgtg aaaaatatca aaaatgaaat tgattcacat gaaagtaaaa taaaagattt
    10981 agaaaaaagt ataataataa aaagtacaag tataaagaat gttgagtctg aaattgaatc
    11041 gttaaaaggt agtattacta aattagaaaa tgagaagaat gatttaaatt ctaacctaaa
    11101 ttatacactt gaaaaaagtg atgatgttag aaaagatatg gaagagttag atgatttata
    11161 caataaaaat aaagaaaaaa ttgatgcatt gaatgaggaa ataaaaagat acaatgattt
    11221 atatgataaa gaaaaatctg aatttgatga attgaactta agtttagtga agaaaacaga
    11281 agtttacaat agtatagtta gagatataaa aagaatatct ggtgaaaatt gtgagctaga
    11341 agaaaaaaat aaacaattag aagagtcatt aaattatgaa gaacatgaaa taattaaatt
    11401 acaagattct atactcactg aagaaaaaga aaaagaaaat ttaacaaaac aactagggga
    11461 tagtaataga aatcttgaaa ctagaaaaat agcaaaagat gatttaaaaa atagttttga
    11521 tgaaattaat aaagaattaa aaactataga tagacagcat atagaactta aagaaagttt
    11581 atttaaagtt ggtggaagat tagaaagatt aaaaactagt caagacacat atataaataa
    11641 gttgtttgag caatatgata tgaccttagt acaagcattg gaaattaaag atgaagattt
    11701 ggatatagat agaaaattct tagaaagtct aaagagagaa ataagaagtc ttggaaatat
    11761 aaatatagac tctataaagg aatatgagga aataaaagaa agatacgatt tttatagcga
    11821 acaaaagcaa gatttagaag agtctatgga agagatagaa aaacttatac atactttaga
    11881 agaaaatatg aaatctgaat ttgaaattaa gtttgaagaa ataagtaaaa attttaaata
    11941 tgtatataag agattatttg gtggaggttg tggagaatta actattttag ataaagaaaa
    12001 tttattagaa agtgatatac taataacagc tcaaccccca ggtaaaaaaa tgaaaaattt
    12061 aaacctatta tctggtggag agaaagcttt aacggcaata agtatattat ttgctatatt
    12121 gattacaaag ccaacaccat tttgtatatt agatgagatt gaagcgccac ttgatgacgc
    12181 aaatatattt agatttggag aatttttgaa ggatttatct aaagagactc aatttatatc
    12241 agtaactcat aggagaggaa caatggaagc tgctgattat atctacggag ttacaatgca
    12301 agaaaaagcc atttcaaaag ttattagttt aaaattaaaa gaagctcagg aaataacaga
    12361 tattatataa cttagattga cataggagga ataacagtgt taaaaaaatt atttggtttt
    12421 ggaaaagaca aggaaaagga aatagaaaaa aaagatgctg aagaagaaat agaagttgaa
    12481 gacagtgttg ataatttaga aaacttggaa gaaactattt tttcaggact tgaagaagaa
    12541 gtgatagata aagtggaaga tattgaagaa aaaaatgagg aaagtgtcaa taatgaaggc
    12601 actgaagaaa tcgaaaactt tgaaaaaata gaagttgaga tggatagtaa tggaaaagaa
    12661 gtagaaaata tttcaaataa tgattctcaa gaagagataa tcgaagaatt agaaaattat
    12721 gatgaaattg agtctaaaga gatagaagat aaagaagata agaaagttaa cttgtttgaa
    12781 agactaaaac aaggattgac aaaagcaaag caaggaataa ctgatagaat agatgaagtt
    12841 ttaaaatcat atacaaaaat agatgaagaa ttacttgaag atttagagga aattttaata
    12901 acagcagatg ttggtgtaaa tacaactatg gatataattg aaagactaag agataaaata
    12961 aagcaaaaag gtattactga accaataaag gtaagagaag aacttaaatc tatagttgaa
    13021 gatattctta caaatgaaaa ctctacacta gatatagaac cagcaccatg tataatatta
    13081 atggtgggtg taaatggggt cggtaagact actacaatag gtaaactagc aaatagatac
    13141 aaaaaagatg gtaaaaaagt tttattagca gcagcagata catttagagc agcagcgaca
    13201 gagcaattag aaatttgggc aaatagaacc aatgtagaca taataaaaca tcaagaggga
    13261 gcagaccctg gagctgtagt atttgatgct ataaaagctg caaaagctag aaaaacagat
    13321 gtattaatat gtgatacagc aggaagatta cacaataagg ctaatcttat gaatgaactt
    13381 ggaaaagtat ttaaaatagt agatagagaa tttccagaag caaaacgtga agtacttctt
    13441 gtggtggatg ctacaacagg tcaaaatgca gttgttcaag caaagacatt taaagaagtt
    13501 gcagatataa caggtatagt acttacaaaa cttgatggaa ctgcaaaagg tggagtagtc
    13561 cttgcagtaa aatcagaagt agatgtacct gtaaaactta ttggagttgg agaaagtgta
    13621 gaagacctac aagatttcaa tgcaaaatca ttttcagatg cattatttgg taactaatgt
    13681 tcttttaaaa ataatggttg acaagagtgt tgttatgata taaaatacaa tagtgtaaag
    13741 taaataacct ttacagtata ctgctaggaa gtgtaagata tgaatataga aaaaatggtc
    13801 gaaattggat tgttatttga acagtataaa gaattattaa ctgacaagca gaaagaaata
    13861 gttgctctat actatgaaga agattattct ttaggtgaaa ttagtgaaaa tctgaatgta
    13921 tcaagacaag gtgtttatga taccttaaag cgttcagaaa aaatactaag agattatgaa
    13981 gaaaagttac acttagtatc aaagattcaa gagcaggaaa aaaatattaa gattataaaa
    14041 gataaaatta ttgacattaa agaagattta ttgcacaata gagattgtgc taatttaatc
    14101 cctaagctag aaaatataga agatgtatgt agggagatga taaaatgata tttgaaggat
    14161 tgtctgataa actacaaggg gcatttagta agttaaaatc taaaggaaaa cttacagaag
    14221 tagatgtaaa aaatgctatg agagaagtta aacttgctct tcttgaggca gatgttaact
    14281 ttaaagtagt taaagatttc ataaaaaaag ttcaagagag atgtgttggg caagaagtta
    14341 tgcaaagctt aactccagct cagcatgtaa tcaaaattgt aaatgaagag cttacaagtt
    14401 taatgggaga tgtacaaagt aaagtaatga tttcctcaaa gcctcctaca attttaatga
    14461 tggttggatt gcaaggtgca ggtaagacta ctacagcagg aaaacttgga ggatatttta
    14521 aaaagcaagg taaaaaacct ctattagtag cttgtgatat ttatagacca gcagctataa
    14581 agcaacttca agttgttgga gaaaaattgg atatacctgt attcaatatg ggagataaag
    14641 aaaatccaat aaatattgcg aaagcaggtt taagccatgc gattaaaaat gctaatgatt
    14701 tagttattat agatacagct ggtagacttc atatagatga agtacttatg gatgagttaa
    14761 agtctattaa atcagaagtt aagccacatg agatactttt ggttgtagat tctatgacag
    14821 gtcaagatgc tgtaaatgtt gcagaaagct tcaatgaggc acttggtgtt gatggagtag
    14881 tattaactaa attggatgga gatactagag gtggtgctgc actttcaata agagcagtta
    14941 cacaaaagcc tataaaattt atggggatgg gagaaaagct agatgatata gaacctttcc
    15001 acccagatag aatggcatca agaatattag gtatgggaga tgtcttaagt ttaatagaaa
    15061 aagcacaaga aaatatagac ttagagaaag ctaaagaatt agagcaaaaa attaaaaaac
    15121 aagagcttga ttttgaagat ttcttacaac aaatggaaca aatacaaaat atgggacctc
    15181 ttgacaagat acttggtatg atacctggta tgggaaatgt aaaagaccaa cttggagatg
    15241 tagatttgaa tggcaaagaa atgaagagaa caaaagccat aattcagtca atgactacat
    15301 atgagagaag aaacccagga gtattaaatg cttcaagaaa gaaaagaata gctaaaggca
    15361 gtggaactag tgtacaagat gtaaatagac ttataaaaca acttaatgaa atgaaaaaaa
    15421 tgatgaagat gtttgctggt tcacaaaaga gtatgaagaa aaggggcggc ttaggcggtt
    15481 tacctttttt taaataaaat ataaaaaagt aaaaattatt cggaggtgac tgtaatggca
    15541 gttaaaataa gattaaaaag aatgggagct aacaaaaaac ctttctacag aatagtagta
    15601 gctgattcaa gagctccaag agatggaaaa tttatagaag aaataggata ctataatcca
    15661 atttctgaac ctaaacaagt aagaataaat gatgaaaaag ctataaaatg gttagctact
    15721 ggtgctcagc caacagaagt agttaaaaaa ttacttgtaa aaaacggagt aatagaaaaa
    15781 ttcgaagctt ctaaacaagc aaaataattt tttcttggca attagtttaa gtaagggctc
    15841 attgctaagg ttactttagg agatgaaata tatgaaagag ctagttgtag atatagctaa
    15901 ggctctagtt gataatcctg actcagttgt tgttgaggaa tttgaagata atgatggtat
    15961 cgtcttaaag cttacagttg ctcaagagga tatgggtaag gttattggga agcagggaag
    16021 aatagctaaa gctataagaa ctgtcgtaag atctgttgca aatagagaaa atataaaagt
    16081 ttctcttgag atagtataaa ataggaggat taggtataat gcctagtcct ttttatataa
    16141 atgcgtagtt taagatttta tgatgatatg accacataag attaaatttt ttaagaaggt
    16201 gataacatga aagaaaaatt aactcatttt aaggtaggtc aaatagtaaa tacacaaggt
    16261 ttaaaaggtg aagtaagagt ttatccgctt actgatgata tagagaggtt tgatgaatta
    16321 aaagatttct atctgggaaa agatttaaat aataaattgg ctgttgaaaa agtaagatac
    16381 aaaggttcta tggttataat gaagataaaa ggcatagaca gcatagagaa agcagaaaaa
    16441 ctaaaaaata aatttatgta tgtagctaga gaagatagca gagagcttga agaaggcgaa
    16501 ttttttatag cagatatgat tggaatggaa gttttaactg tagatggaaa atatgtagga
    16561 atactagaag atgttcttca atactcagca aatgatgtct atgtagtaaa aggtgaggaa
    16621 gataaagaat ttatgatacc agctataaaa aaatttgtac ctacaataga tattgatgaa
    16681 agaaaaatga ttatagaccc tattaaggga atgatagatt aggtggtaat atgagatttc
    16741 atataatgac actttttcca gaaatattta attcgtacat ggatgaaagt ataatgaaga
    16801 gagcagttga aaaaggtata attgaggttc atatatataa tataagggat ttttctaata
    16861 ataaacataa aaaagtagat gattatccat ttggaggagg agcggggatg gtaatgactc
    16921 ctcaacctat atatgacaca tataagcata taataacaac tcataatata aataacccaa
    16981 gtgtaattta tcttactcca aaaggtaagg tatacaatca atctatggca aagcagatgt
    17041 ctttaaaaga agatataata ctactatgtg gtcattatga aggaatagat gagagaatca
    17101 ttgatttaat agttactgat gaaatttcaa ttggagatta tgtattaact gggggagagc
    17161 tcccagcact tataatgata gattctatat cgagattaat accaggtgta ttaaaccaag
    17221 aggaatcatt tgaagaagaa tcatttaaag ataacttatt ggaatatcct cattacacta
    17281 gacctagaga ttttgaagga ttaaaagtac cagaagtgct tttatcaggg aatcataaga
    17341 agatagatga gtggagaaga gaagaatcta taaggataac taaagaaaga cgatttgatt
    17401 tatataaaaa gtctaatgaa aaataaaaat ataatgaaaa attaatatat atgtatacaa
    17461 taaagtacct ataaatggaa ttaaaaacat aaaagtgctt gtaataacta ttgaagtata
    17521 gtataatata aaatgttgaa aatacgggcg ttcctctgtt tacactggtc gggtagttaa
    17581 gaacgtctat gatatctagg aggaagaaag atgaatgaaa tgctaagagc aattgagcaa
    17641 gaacaattaa aaaatgaagt tcctaatttt ggacctgggg acacagttaa agtacacgtt
    17701 agaattatag aaggaaaaag agaaagaata caagtattcg aaggtgtagt attaaagaga
    17761 caaggtggag gagcaagaga aactttcaca gttagaaaaa tgtctttcaa tgttggtgta
    17821 gaaagaactt tcccagttca ctctccaaaa atagaaaaaa tagaagttac tagaaaaggt
    17881 aaagtaagaa gagctaaact aaactactta agaggtagag taggtaaagc tgctaagata
    17941 aaagaagcta gaaataaata attaaaaact ttacttataa atggggggct gatttatcag
    18001 tcctctttct attttaatga aaattacagc aaataattat actagaaata aatagaaata
    18061 tttgttgaaa atatacatta tttatctatg aatttatagc ttttaaaatg aaaaataaaa
    18121 ttggtaaata ttgcattata ttttgtaaca atattaaaaa taagagaaca gtattatgta
    18181 aagtatgacg ttaaaaagat gacaatatta tagtttgtta agatagattt tttaagtgga
    18241 attaggatat aataatataa tattaataag gagtgataca tgtgtcaaat gaatacgaag
    18301 agtatcttat gaatgacaat ctacatataa actggtatcc aggtcatatg aaaaaaacta
    18361 aggagttagt aaaaaataac ttaaaattga ttgatgtagt aattgaactt ttggatgcta
    18421 gaatcccgtt tagtagtaaa aatccagaca tagacaggct tgtaggagat aagccaagag
    18481 ttgtcgtact taataagagt gatatggcag atagggataa attgaatcaa tggattgaat
    18541 actataagaa aattaatatc aaagctatac cagtagatac aataaaaggc gttggtataa
    18601 ataagataat cgaagaatgt aaaaatgtaa ctagagaaaa aatgagttct ttaaaagaca
    18661 aagggagaaa agaaagagct atacgaataa tgatagttgg agttccaaac gttggtaaat
    18721 cgtctcttat aaacaaatta acaggtagaa aaagcactca aactggagat aagccaggag
    18781 taacgaaagg gaaacaatgg gttagattaa agggaaatct tgaactttta gatacacctg
    18841 gaatactttg gcctaagttt gaagaccaag aagttgcatt aaatttagca tttagtagag
    18901 ctataaagga tgagatttta gatactgaaa ctttggcttt aagacttata gagaaactta
    18961 tgaaaataga acctgagaag ttgaaagcca gatataagtt agattgttta ggagaaactc
    19021 caattgaaac tatggatatg attggccata aaagagggtt tataacaggt aaaaaagaac
    19081 tagattatac acgcatagcc acaactgtat taaatgaatt tagagatgga aaaataggaa
    19141 atataacatt ggaagttcca gaaaacgtta aaagatagat tttaaaacaa aatcaaaaat
    19201 ttaatgttaa aaatttaaaa aagcctttta gcaaaggctt ttttattttt ttgaaatata
    19261 gacaaatatc tccatataaa agtataaatt aggttagaaa acctttaaaa atattaattt
    19321 aagacaaata tacctaatat tttgaaaatt aaaatgagtg tgattattta aaattttagc
    19381 atattgactt gcttgtttcg tataatatat aataagcaca aataaataca atatattaaa
    19441 aatttaattt atttaatttt attaatttaa taataagttc ttattgttta attcaactta
    19501 cttatgcaag cgtaaacaat cgagacattt tattaattta ataaggttaa aaagggggat
    19561 tttataatgg gtaagaccaa ggatgttata gtatttggat ttgcgttatt tgcaatgttc
    19621 tttggagcag gtaacttaat atttccacct tacttgggaa taattacagg tccagaatgg
    19681 ttaattgcct tcttaggatt tacgtttgct gatgctggat tggcacttct agcagtaatg
    19741 gctacagcta aattcgatgg aaacgtagtt gaaatgttta aaagatgtgg tatgaagtta
    19801 gggattctta taggttgtgc agatatttta tgtataggcc cattcttagc tataccaaga
    19861 acaggtgcaa caacttatga aatgggtata atgcctttgt ttggaacttc aattccagtg
    19921 ttattattct gtatactttt ttttgcgatt tcatatgtac taacaataag accttctaag
    19981 gtcgtagata tagttggaca gtttttaact ccagctcttc taatagcttt agcatttata
    20041 attataaaag gtattatatc acctcttgga gatatagtag ataaacctat gatacctaat
    20101 gtatttgcag aaggtatagg tcaaggatat cagacaatgg atgcttttgc agcaatagca
    20161 cttgcatcag tgctaatagt atctttaaat gataaaggat attctactat tagtgacaag
    20221 ttgaaaatga ttggtaaggc gggtgttctt gcttgtggag gattagctct agtttatgga
    20281 ggattatgtt tccttggggc aactgtatca acaatgtatg gaacagatgc agtacaatca
    20341 caagtaatag taaatataac tgaaggattg cttggaaatg ttgggaaagc gattttagca
    20401 gtagtagtat cattagcatg tttaactaca tctataggtc ttacatctgc tacaggtcaa
    20461 tatttctcta gacttacaaa aggtaaattg agttatgaaa aaatagtatt agctgtatct
    20521 gtattcagtg cagtagtagc atcttttggt gttggaacta taataaagat agcatctcct
    20581 atactaagta tagtttatcc accatcaatt gttttaataa ttttagcttt cttcaatgaa
    20641 aaaattaaga atgataatgt atacaaagga gcagtatata tgtcattgct tgtaagtata
    20701 ctaacagtta tatctagtta tggagtagct gttccagtag taaattcttt acctcttaat
    20761 tcattaggat ttaactgggt ggtgccagtt ataatagctg gaataattgg taattttata
    20821 ccatcaaaga gtcaatctaa tacacttgga acaaactaag ttttgtttaa ttaaaatata
    20881 atcaattgta aataaatgag ttaactaaaa gtaaaaggag ttatttgtac aaataactcc
    20941 ttttattttt agattctata tttaattatt tgtatttaat atggaaaaag tataagaaaa
    21001 tatcaaaaaa atatatagtt tattttttaa caataataga gattaaaaaa gaattttaga
    21061 gtaattttat aaatttaagt atacatatta aatgattttt acaaaaatat aatataaata
    21121 aaattgtgac aataataaaa taataaaaca aaatttaaga catatgatta tataaatgta
    21181 ttaaataaat gagtaaattg ttttcattga gagatatatt aagaattttt agttaattat
    21241 gacatataag tatgtaaacg tgaatactag caactgcaaa gtttaagtaa gttggaagtt
    21301 agagagtaat tgttatgaag aaaaataagt tgataaatca taataaggaa ttttcagaaa
    21361 aaatggtttt aagagtaaaa aacaagaggt aaatctacgc aagtagaata aaaattgaat
    21421 aaagttaact gtcaatttag caaaataaat acaaaatact acaagaatta acgtaaatag
    21481 gtgttatgat aaaaggaaaa cgattttttg agaatcataa ttttgaaaat taaaaaaatt
    21541 aaaaatgcta ttgactgctt ttttttcaga ttatataata aacacaaaat atttaaaatt
    21601 taaattgaat taaaaagatt atatgaatgt aaaaaaggag agtgatatat acagtatata
    21661 aagcaataca acttaataat aaatcgaata atctaaaaaa tcttaatatt ttaattattc
    21721 ggaatagtta attttatcaa gaaaaattag agaaaaaaca aaaaaccaac tatgtttaaa
    21781 gtatacaggt tgattttgtt taattaagaa agttgtatta aatattgtga agattttata
    21841 aattatgaaa cataattgac aaatttaagt caaaaatgta taattaaata aaatctcgtc
    21901 aaaaggtttt catttaatta agagattgga cttatttttt agtaaaaggt agtaaaattc
    21961 aaatgggggt gctttaaatg ggtgagaaac aaatcaattt taaagatgta attgtaattg
    22021 gatttgcact atttgcaatg ttttttggtg cagggaactt aatcttccct ccatatctag
    22081 gagttttatc tggaagtagt tggctagtag ctttcatagg attccttttt gcagatggtg
    22141 gtttggcatt attagcagtt atagcagcaa ctaagttcaa tggagacaca tcgaagatgt
    22201 tctcaagagc aggcaaagga ttaagtattg cattaggatg tgcaatggta atctgtatag
    22261 ggccactttt agcaatacct agaacagcag caacaactta tgaaatgggt atattaccta
    22321 caataggttc tggaataagc ccagtaatat tctctatagt attctttgct ctagtattgg
    22381 ttttaacaat aagaccatca aaagttgtag atatagttgg gtcaatatta actccagctt
    22441 tacttatagc tttagcagta ttaatagtta aaggtatcgt atctccatta ggagaaataa
    22501 gagacagttc tttaatacaa aatgtatttg cagaaggtat aactcaaggt tatcaaacaa
    22561 tggatgcatt ggcagctagt gtatttgcat caattataat catgtcagta atagctaaag
    22621 gttatacagg tgagaaagaa aagatgaaag ccacaataag tgcaggtgta attgcagtaa
    22681 taggaatggc attagtttat ggtggattat gttatcttgg agcaactgtg tctcaacaat
    22741 atggacaaga tgttcaacaa acagctttaa tagttgcaat aacagcagct ttacttggaa
    22801 ctacaggtaa aattttattg gctattatag ttgcattagc atgtttaaca acagctatag
    22861 gtctttcttc agcagctggt caatatttct caacacttac agatggaaaa ttaaagtatg
    22921 agcatatagt tatagtagtt tgtgtattta gtgcaatcat atcaaacttt ggagtaagta
    22981 caataataca attctcttct cctatactaa gtatggtata tccagcaact ataactctag
    23041 ttatactagc attatttagt aataaaatta aaaatgacaa cgtgttcaaa tgtgctgcat
    23101 acatggcatt attagtaagt gtattaacag ttgcaacaag ttttggtata aaaattcctc
    23161 tagttaatag cttaccattt gcttcattag gatttaactg gatagttcca gttgtaatag
    23221 caggaataat aggaaacttt attccatcta aaagcagtaa aactgcataa taataaaaac
    23281 taattgataa ttatggaatt gcctttaaat tatataggca attccttttt atatttaaaa
    23341 gaattattaa attattgtag ataaaagaac attattaatc ctatttgata aat
//
LOCUS       DAGSRM010000056        23238 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.056, whole genome shotgun sequence.
ACCESSION   DAGSRM010000056 DAGSRM010000000
VERSION     DAGSRM010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23238
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.056"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            85..1371
                     /locus_tag="MJE33_002348"
     CDS             85..1371
                     /locus_tag="MJE33_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBY9648280.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            1483..1956
                     /locus_tag="MJE33_002349"
     CDS             1483..1956
                     /locus_tag="MJE33_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1615..1617,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HBY9648281.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            1988..3298
                     /gene="grdB"
                     /locus_tag="MJE33_002350"
     CDS             1988..3298
                     /gene="grdB"
                     /locus_tag="MJE33_002350"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:3035..3037,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HBY9648282.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            3554..5086
                     /locus_tag="MJE33_002351"
     CDS             3554..5086
                     /locus_tag="MJE33_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III family protein"
                     /protein_id="HBY9648283.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNPDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            5097..6242
                     /locus_tag="MJE33_002352"
     CDS             5097..6242
                     /locus_tag="MJE33_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HBY9648284.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
     gene            6552..7631
                     /locus_tag="MJE33_002353"
     CDS             6552..7631
                     /locus_tag="MJE33_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBY9648285.1"
                     /translation="MSRVKNVVELLETKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYVKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATKLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGGASEKQLEIYNLVKEAQNVGVENMKAGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            7744..8343
                     /locus_tag="MJE33_002354"
     CDS             7744..8343
                     /locus_tag="MJE33_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648286.1"
                     /translation="MYKKHKFIKNIKKAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSANFIKRPLRKYTNTDVSSNLFIDNNLSPKGQE
                     SNQNNIMNVKNTIGFTSEADNYEKKIPINNNEGFEEGETSELQIIIATVICIVALTVL
                     FVVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSKPD"
     gene            complement(8529..9401)
                     /locus_tag="MJE33_002355"
     CDS             complement(8529..9401)
                     /locus_tag="MJE33_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBY9648287.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERAITANIGVLWRRNQYISHS
                     MKLFIEYMASIHIN"
     gene            9818..11221
                     /locus_tag="MJE33_002356"
     CDS             9818..11221
                     /locus_tag="MJE33_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648288.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVVNFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            11248..12792
                     /locus_tag="MJE33_002357"
     CDS             11248..12792
                     /locus_tag="MJE33_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBY9648289.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDGMVIGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEEGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGVVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKAGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            12823..14214
                     /locus_tag="MJE33_002358"
     CDS             12823..14214
                     /locus_tag="MJE33_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HBY9648290.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKVQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            14407..15903
                     /gene="abfD"
                     /locus_tag="MJE33_002359"
     CDS             14407..15903
                     /gene="abfD"
                     /locus_tag="MJE33_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HBY9648291.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            16038..16313
                     /locus_tag="MJE33_002360"
     CDS             16038..16313
                     /locus_tag="MJE33_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648292.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENVEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            16337..17644
                     /locus_tag="MJE33_002361"
     CDS             16337..17644
                     /locus_tag="MJE33_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBY9648293.1"
                     /translation="MSWQELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPAFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVRESLAVEFEKRFKEKY"
     gene            17664..18782
                     /locus_tag="MJE33_002362"
     CDS             17664..18782
                     /locus_tag="MJE33_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HBY9648294.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVSPKKVKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
     gene            19069..19989
                     /locus_tag="MJE33_002363"
     CDS             19069..19989
                     /locus_tag="MJE33_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBY9648295.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILPIQVCAVFLLIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFVPPRYEDVAYEVKEEEK
                     TDESNEVDTIINKGMNYLTQIKEANKNIKSESMGDKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            20104..21261
                     /locus_tag="MJE33_002364"
     CDS             20104..21261
                     /locus_tag="MJE33_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBY9648296.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(21346..22845)
                     /locus_tag="MJE33_002365"
     CDS             complement(21346..22845)
                     /locus_tag="MJE33_002365"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBY9648297.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIENEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
BASE COUNT         8951 a         2740 c         4383 g         7164 t
ORIGIN      
        1 aaaaataaga taaattgtaa aatgatataa ttttgagaaa aactcaaatg catatacaca
       61 aattaaataa caggaggtgc aagtatgcgt cttgaattag ggaaaatctt tataaaagat
      121 attcaatttg gtgatgtaac agaagtaaaa gatggagtat tattcatcaa taagcaagag
      181 atgctacaag aaattggtgg agatgagcat attaaatcta tagatataga attagctcgt
      241 ccaggtgaaa gcgtaagaat aacacctgta aaagatgtta ttgagccaag agtaaaagtt
      301 gaaggtaatg ggggaatctt ccctggtatc atgtctaaag ttgacactgt aggagaagga
      361 aaaactcatg ctttaaaagg tgtagctgtt gtaactactg gaaaaatagt aggtttccaa
      421 gaaggtataa tagacatgac tggagaagga gctaagtaca ctccattttc taaattaaat
      481 aatgtagtag taatagctga acctattgat ggtttaaaac aatacgctca tgaaaaagct
      541 gttagaatga taggattcaa agcagcaatg tacttaggag aagtagcaag aaatcttact
      601 ccagatgaag tttcagtata tgaaactaaa cctcttcttg aatcaataaa agaatatcca
      661 gagcttccaa aagtaggata tgtttacatg cttcagactc aaggtttatt acatgatact
      721 tatgtatatg gagtagatgc aaaacaaata gtaccaactt tattatatcc aacagaatta
      781 atggacggag caattgtaag tggtaactgt gtttctgcat gtgacaaaaa cccaagttat
      841 gttcatataa acaatggggt tgttgaagac ttatatgcaa gacatggtaa agatataaac
      901 tttgtaggtg taattataac taatgaaaat gtttatctag cagataaaga aagatcttca
      961 aactggactt ctaaattatg taaatattta ggattagatg cagttatagt ttcacaagaa
     1021 ggttttggaa atcctgatac tgaccttata atgaactgta agaagataga aatgcaaggt
     1081 gttaaaacag ttatagttac agatgaatat gctggacgtg atggaggttc tcaatcactt
     1141 gcagatgcag atgtaagagc tgatgctgtt gtaacaggtg gaaatgccaa ccaagttgta
     1201 gttcttccta agttagataa agttattgga catttagaag tagtagatgt aatagctggt
     1261 ggttctgatg gaagtttaag agctgatgga actattgaag ttgaaatcca agctataaca
     1321 ggtgcaacta atgaaacagg atttggtcat ttaactgcta aaggatatta agaagtattt
     1381 tattaaacga ttgagtaatg ttaataaaac atattataga taattataat aagtgttata
     1441 gatatataaa aatatattag aaatataata aggaggatta ttatgagttt acttagtaat
     1501 aaaaaggttc ttataatagg tgaccgtgat ggtataccag gacctgcgat agaagaatgt
     1561 gtaaaaacag tagaaggagc agaggttgtt ttctcatcta cagaatgctt tgtctgaaca
     1621 gctgctgggg ctatggactt agaaaatcaa aacagagtta aagatgctgc tgataaattc
     1681 ggagctgaaa atgttgtgat tttactaggt gctgctgaag ccgaagctgc aggtcttgca
     1741 gccgaaacag taactgctgg agatccaact ttcgctggac cacttgctgg agttgcctta
     1801 ggattaagtg tttaccacgt tgttgaggaa ccaataaaat cattatttga tgaaagtgta
     1861 tatgaggacc aaataagtat gatggaaatg gttttagaag ttgaagaaat agaagaagaa
     1921 atgtctggta taagagaaga attttgtaaa ttttaatgcc taaaagcaaa aggggtgaaa
     1981 ctaagagatg ggaaaactaa aagcagtaca ttatattaat cagttctttg ctggtatagg
     2041 tggagaagaa aaagctgata ctaagccaca tgtggcagaa actttacctc caataagttt
     2101 acaacttgat aaattattag gtgaagatat tgaaatagtt ggaacagtag tttgtggaga
     2161 tagctatttc aatgaaaata tagatagtgc aagtgaagaa gttttatcaa tggtaaaagg
     2221 ttttgaacca caacttttca tagctggtcc tgcatttaat gctggtagat atggggttgc
     2281 tgctggaaca ataactaaag ttgtaaaaga tgctttgaat ataccagctt taacaggtat
     2341 gtatgttgaa aatccaggag cagatatgtt caaaaaggat gtttatgtag tagaaacttc
     2401 tgattctgca gcaggtatga gaaaggcact tcctaagata gctaaacttg cagtaaaact
     2461 tgctaatgga gaagaaattg gaactcctaa agatgaagga tacatagcta gaggaataag
     2521 agttaactac ttccatgaag acagaggttc taagagagct gttgacatgt tagttaagaa
     2581 aataaaaggt gaaccatttg aaactgaata cccaatgcca aactttgata gagttgaccc
     2641 aagtaaagct gtaaaagatt tatcaaaatg taaaatagct ttagttactt ctggtggtat
     2701 agttccaaaa ggaaatccag atagaataga gtcttcttca gcttctaaat atggaactta
     2761 ttctatagca ggagttatgg atttaacaga agagacttat gaaactgcac atggtggata
     2821 tgacccagtg tatgcaaatt tagatgcaga tagagtatta cctgttgatg ttcttagaga
     2881 tttagaaaaa gaaggtgtaa ttggaaaatt acatgagact ttctatacaa ctgtaggtaa
     2941 tggtacatct gttgcaaact ctaaaaaata tgcttctgaa attggagcag cattagtggc
     3001 tgatggtgta gatgcagtaa tattaacttc tacatgaggt acttgtactc gttgcggtgc
     3061 aacgatggta aaagaaatag aaaaaactgg acttccagta gttcatatgt gtacagtagt
     3121 acctatttca ttaacagtag gggctaatag aatagttcca actatagcaa tacctcatcc
     3181 acttggaaat ccagcgcttg accctacaga agaaaaagcc ctaagaagag gccttgtaga
     3241 aaaagcatta aatgctttaa ctacagaagt agatggtcaa acagtatttg aaaaataaaa
     3301 ataacacaca catatattat atagataaaa atataaacac acactaaaaa taaaaaataa
     3361 ctatacacaa aaaaataata tagatttcat gaatggataa attggagtta ttcattcatg
     3421 aaatcttaaa aaatgccaaa atacataaac aataatagaa tcaaatatac aataaaatac
     3481 aatgattaga gcttttaata agtttaataa gattaaaaaa taagtgttca taaaatttat
     3541 ggaggtgcac aaaatgactt atccagtttt aaaaggggca ggctatgtac ttattcatac
     3601 tccagatatg atagtgcaaa atggaagtac ttgtacagtt gaaagagcaa caaatccaga
     3661 ttcagaattt ttaaaagaag taagtaatca tataagaagt tatgaagatg tagttaacta
     3721 catgccaaat caagtttata taggaaatag aagaccagaa gaattaagag atttaccaat
     3781 gccatggtgt gaacaaaaga ttgagggaac tagaaatggt aagtttggag aaataatgcc
     3841 tcaagatgag ttcatagctt taatgcaaat aagtgatgca tttgatttag taaaattatc
     3901 acaagaattt attgatgaag taaagccaaa aatcgaaaat aactaccctg aaattgcacc
     3961 atttgtaggt aaattaaagg gtgatgatat agaagaaggt aaagaattag tagcaacaca
     4021 catagctgaa ggattatacc atgatggaaa atttgttggt tatgtaaaaa gagcacatga
     4081 tgtagatgtt aacttaaatg cccatacaat gtttgaaaat ctagtagtaa aagcatctgg
     4141 agttttatct gctattcaaa tgctaagaca tagcaaaata gaccctgctg aaatagatta
     4201 tgttattgag tgttcagaag aagcttgtgg agatataaac caaagaggtg gaggaaactt
     4261 tgctaaatca atagctgaga tagcaggact tcaaaatgca actggttctg acacaagagg
     4321 attttgtgca gcaccaactc atgcattaat tcaagcagca gctttagtaa aagctggcat
     4381 acataaaaat gtaatggtag tagcaggtgg agcaagtgct aaacttggaa tgaatgctaa
     4441 agaccatgtt aaaaaaggtc ttcctgtact tgaagatgtt gttggaggat ttgcagtact
     4501 tgtatctgaa aatgatggtg taaatccagt tataagaact gatttaacag gtaagcatac
     4561 tgttggaact ggttcttcac cacaagcagt tatgacagca cttataactt caggtcttga
     4621 tagagctaat ttaaaaataa cagatgttga tgtgtattca gttgaaatgc aaaacccaga
     4681 tataactaag ccagcaggag caggagatgt tccagaagca aactacaaaa tgataggggc
     4741 acttgctgtt aaacgtggag acttagaaaa gaaagaattg aaagattttg tttctaacaa
     4801 aggattacca ggatgggcac caactcaagg acatatacca tcaggagctc cttatatagg
     4861 atttttaata gatgatttaa ctacaggaaa tagaaataga gctatgatag ttggaaaagg
     4921 tagtttattc ttaggaagaa tgactaactt atttgatgga gtatctttta tagctgaaag
     4981 aaatacaggt gttactgaag agacttcagg aatatctaag gatgaaatta agaagataat
     5041 agctgaatct atgaaaaaac tagcattaga tatgttagaa gaataggggt gaggatatgt
     5101 caaaaaaagt aatcgcagat gtattcttag aagtagctaa tgcaatagaa agcggcgaat
     5161 ttggcaaaaa agtaaaaatt ggtgttacta cacttggaag tgaacatggt gtagaaaaca
     5221 tggttaatgg tgcacaatta gcaaaatcta atttatttga tattgtgctt attggaccaa
     5281 aagtggaaac tgaccttgaa gtagttgaag ttaatgatga aaaagaaatg catgcaaaaa
     5341 tggaagaatt attagattca ggatatattg atgcttgtgt tactatgcat tataattttc
     5401 caataggtgt atctacagta ggtagagtaa taactccagc aaaaggaaaa gaaatgatac
     5461 ttgcaacaac aacaggaaca agtgctacta atagaataga ggcaatggta agaaatgcca
     5521 tttatggtat tgctacagct aaatcaatgg gtaacaaatg cccaaaagtt ggaatactta
     5581 atgttgatgg agcaagacaa gttgagaagt gcttaaaaga attaaaagat aatggatatg
     5641 acatggagtt tgcggattca attagagcag atggtggatg tgtaatgaga ggtaatgatt
     5701 tacttgtagg tgcacctgat gttatggtta ctgatacatt atctggaaat atatttatga
     5761 aagtattttc ttcatatact acaggtggag attatgaagc tcaaggcttt ggatacggac
     5821 ctggagttgg agaggactat gatagaaagg tacttatagt atcaagagca tcaggttctc
     5881 cggtagtagc aaatgcttta aaatacgctt atgatgttgt aaagggagat ataagtaatg
     5941 ttgcaagaaa tgaatttgca aaggttaaaa aagcaaaatt tgatgacatt atatcctctc
     6001 ttactaaaaa agaagttaaa gctgaaaaag tagaagttaa aatgccagat aaagaaattg
     6061 ttactcgtca aatagctggt gttgatataa tggatttaga agatgcagta tctgagttat
     6121 ggaaaaacgg aatctatgct gaaagtggta tgggttgtac aggtccaata gttttagtaa
     6181 atgaagctaa aggagactta gcagttgaaa ctttagttaa agctggatat actgcaaaat
     6241 aagtacattt tttatatgta aataagaaca tatagttata attttattga ttataattat
     6301 atgttctttt tattttatat cttgtcaata tttatatatt aacacaatcc atttatttga
     6361 ttctctagct aatttattat atttattcag ttaaaattaa gctttctaac taaataaaaa
     6421 ctaatgaagt attaaaatga aattgataaa atatgaaaaa attttaggaa taaagtatct
     6481 gttagataaa aataagatta attagtgata taatataatt aagataaaat ttattatttg
     6541 gaggcaaaat catgagcaga gtaaaaaatg tagtagaatt gttggaaact aaaggtgttg
     6601 atgcactata tctaacaaag aaaactaatg taaactatat aagtggtttt cctgatgaag
     6661 aagcatatgc agtgatttgc aaagatggaa actttttagt tactgatagt agatatatgg
     6721 agcttgcaga aaaggtttgc aaagattttg aaattataaa ttggcataat tttgatagaa
     6781 gtgtggcaaa ggcagttaaa agtgtatgtg ataaagtggg tataaagaaa cttgggtttg
     6841 aaagaactaa tatagtgttt gataaatatg aagaacttaa aaatctgatt gagaaggata
     6901 atggagaatt aatacctaca gaaaatattg tagagacttt gagatatgta aaagacaaag
     6961 atgaaatcaa aaatacaaga aaagcttgtg aaatagcgga taaggcatta gaagaattga
     7021 ttccacatat aaaagctgga gttagcgaaa ttgaactggc tacaaagtta gagtacttta
     7081 tgaaaatgaa cggagcacaa aatatagggt ttgaaactat actgatatca ggagcaaaga
     7141 cttcattgct tcatggtaag cctagtgata agattataga aaaaggtgat tttgtactta
     7201 tagactatgg cgctatgtat aatggctata tatctgatac aactagaact tttatagttg
     7261 gaggagcttc tgaaaagcaa ttagaaatat ataatttagt taaggaagct caaaatgtag
     7321 gggttgagaa catgaaagct ggtgtacatg cgacaattcc agatgctgag ataagaaaag
     7381 ttgtcaaaaa atatgaagac tattactatc aaggtatagg acatggagtc ggtagagatg
     7441 ttcatgaaga acctttcatt ggtaattatg gtgataaaat catagaagag ggatgtataa
     7501 taacaatgga accaggtatt tatttccctg gatggggtgg agttagaata gaagatactg
     7561 ttttgattac taaaaatggt ccagaaagac ttactaaatt ccctaaagat ttgatgattt
     7621 tagacaaata atttaattta tatatatttt tttacataaa atataacata atctcactat
     7681 taagctctat ttacatatta tagtaaagag ggcttaattt tattgtgaga ggtgatagta
     7741 tatatgtata aaaaacataa atttataaaa aatataaaaa aagcctggta taaaatcaaa
     7801 ttaattgata gatgccttat aattgtaatg gcaatattaa tgtttcaatc tatatataat
     7861 ttatttgtta atgaagtaaa ttcacaagat actacaacta ttgatgtagt agtaagaacc
     7921 acttcagcag ctatatttgg gtatttttta agtgctaatt ttataaagag acctttaagg
     7981 aagtatacaa atactgatgt atcttcaaat ctttttatag ataataattt atccccaaaa
     8041 ggtcaagagt ctaatcaaaa taatataatg aatgtcaaaa ataccattgg atttacttca
     8101 gaagctgata attatgaaaa gaaaatacct attaataata atgaaggttt tgaagaagga
     8161 gaaactagtg aattgcaaat tattatagct actgtaattt gtatagtagc attaacagta
     8221 ctttttgttg taagaaattt tacaactaca acatcagctt ctttaggtac tatttcacag
     8281 atgagagatt ttgtctcagg atgcgtgggt tttttgctag gttgtccaag taaacctgat
     8341 taaaaaagta ttatcaataa aaggtatttt aaaaagtatc agtataataa tatttagatt
     8401 taaatgtata caattataat tctaaacatt ctatattgta tttttatact gatactctaa
     8461 actatactat ttattaaaat attttaacat tgtatttaaa caaaatatta tatctgtata
     8521 atataaattt agtttatatg aatagatgcc atatactcaa taaaaagttt catactatga
     8581 gatatgtatt gatttcttct ccaaagaacc ccaatattcg cagtaattgc acgttctaat
     8641 ggtattgaac agagattttc tgtattttga aatatttctt tatacaatat tgttgaagcc
     8701 aagtcatttt ctagtaaata acgtatggta gaaagttgat ttgagtgtaa aattatattt
     8761 ggcttagctt tttcaagtct ttttgtaata atcttatagt gataagaacc ttcagataag
     8821 attacaaaag gtacattttt taacatataa ttcgatataa tgggatgttt tgccaattca
     8881 tttttgggat ttgtaactaa gtataattct gttttataga ttgtctttga atctatatca
     8941 tcataaatat cactgtcaat tataccaata caaaaatcaa gtgtagattc ttcaatcatt
     9001 ttagctccaa caaatgttgg aacttcaaaa attttaagct ctatatctgg ataaattaca
     9061 ttaaatctag gaagtatttt tgaaagcaaa aacgtaccta aaactgtagg aatacctatt
     9121 ttgagagaag cttttctctt ttttcctaaa tcaagtgcgt cacattgaaa atcctcaaat
     9181 tgttttataa aatgttttga cttgtctaaa aaaatttttc cttcaggcgt taactctatt
     9241 ttattaccta ttctgtaaaa aagttcaaag ccaagttctt tttctaattc tttaattgct
     9301 aatgttatgg atggttgaga aatatgaata ctttccgcag ccttagtcat gtttttatat
     9361 ttacagacag tttgaaaata gtataaatag tttatattca tttggtatta gttcctttca
     9421 tttacatatt agtgacaagt atataattaa caatagcata agttttgtat attgttgtaa
     9481 aaaaatagaa tagttttaaa ctatcatagc ataagttttt gatattttca atctattaag
     9541 aatagtgcta ttattaaatt aacaaaagag aatagctggt atggtttaga gtaaatcaat
     9601 ctatattaca gttatatcaa cgaatatcaa aaaattatct attagaaaat aaaaattatt
     9661 tttacaatta aattgttaat taaatcataa agaaaaaagt gatattaaca agataaaatt
     9721 tttttaatca gaaatgttaa tttaataaca aaatagagta ttggggagaa aggagaaata
     9781 tattttttaa taatatttgt taatttaata acgaaaaatg aaagggagtg atttagtgaa
     9841 gtttgaattg atggattttt taatgaatcc ttttgtcctt atgtttgctg cagtaattac
     9901 aggaatttta tttggaaaaa ttaaatttgg gaagtttaat tttggggtat caggagcatt
     9961 atttacagga ttatttattg gatggcttgc atatagcttg gggaatctaa taatagcaaa
    10021 aggggaaaca gcagcaggat ataaggcagc aactgtaatg atgggtaatg gaattatttc
    10081 ttctgacttc tttgatttct ttttaataat atttgttgca gcagttggtc tattggcagc
    10141 aaaagatatg aaagctgtat taaagaagta tggagcaaga tttgtaattt taggagttct
    10201 tataactttt ataggtggct ctatgactta tgcaatgact ttactatcaa gtgataaagg
    10261 aagtagtgct tatgaagttt ctggcgttta cacaggagca ttaacaagtt cgcctggatt
    10321 agctgctgct ttagaaacag ctggtaaaca tgcagaagat gtatcaaaag aatttgaaaa
    10381 agcttcaata aaagataaaa aagaaatttt aaaagtagtc gacccagaag gcaaattgga
    10441 tgtgaataca actacatcac tgactcaaga gcaaatagat aagtatattg catatgctga
    10501 agctggtgtt ggtataggtc atgcagtcgc atatcctttt ggagttttaa tagtaatctt
    10561 aggtgtaaat ttccttccaa aattatttag gatggaccta aaagaagaaa gaagaaaata
    10621 tgagaaggaa atgaaggaag caagagatag tgtttctggt aaaacgatac cagaagttcc
    10681 atttaatata ataacatttt tcttgacatg tttagctgga tacttagttg gaggaataca
    10741 tgtatttatg ggaccactag ggtacttcac actaggtgca acaggaggtt cactaattgt
    10801 atctctagtt cttggatata taggtaagat aggggtagta aatttcagaa tggaagaaaa
    10861 ggttttaaat attttaaaac aaatagggct agtatttttc ttagcaatag ttggattaag
    10921 atatggtgga aaagtagttg attcaattat gacatctggt atgcacctag ccttagtagc
    10981 aatagcagta ggtgttacag ctatgatgat aggattctta gttggcaaat acgtatttaa
    11041 attaaattgg attttattat ctggagctgt ctgtggtggt atgacatcaa caccaggtct
    11101 tggagctgct gttgatgctc ttgatagtga tgacccagca gcaggttatg gtgctacgta
    11161 tccatttgca ttattaacta aagttatatt ggttatagtt ttacacaaac ttccaatgta
    11221 aataagattt tatatggggg gatatttatg aataatgaag gagcatttga agaattaagt
    11281 ggaagattaa gaaataaaga attgtgtgat ttaataatga atgcagatga agctgctaaa
    11341 attatagagg atggcatggt tattggagtt agtggattta caccttcagg ctatccaaaa
    11401 gcagtaccat tagcagtttc agaaagagct aaaagtggag aaaagataaa attaaccgta
    11461 tattcaggag catctttagg tccagaagtt gatggagcat ggtcagaagc aggtataatt
    11521 gaaagaagac ttccttatca aactaattca atacttagaa ataatattaa taaaggtgtt
    11581 gttgattata tagatatgca cctaagtcat tctactcaat ttttaaatta tggaactata
    11641 cctaaagtag atgtagctat agtagaagca cttgcaataa ctgaagaagg aaatattata
    11701 ccaactagtg ggataggtaa ttctccatct tttataaaaa gtgcagataa agtaatagta
    11761 gagataaatc ttgcaaaacc aatggaaatg gaaggtatgg cagatatata tattacggaa
    11821 aatccaccaa atagaaaacc aatagaaata aatcatccta aggatagaat tggaacaaca
    11881 tatatacctt gtggtttgga taaaattgca ggagtagtaa ttaccaatat gcaagataag
    11941 acccgtccat tgggagttgt agatgaagct tcgaaaaaaa tatcaaataa tattattgct
    12001 tttttaagag aagaagtaaa ggcaggcaga ttatcaaaga atttacttcc tcttcaatct
    12061 ggtgtaggaa gtgtagcaaa tgcagtatta tatggattat gtgaatctga atttgaaaat
    12121 ttaacttgtt atacagaggt tgtacaggac tctatgttgg atttaataag aatgggtaaa
    12181 gttacaatgg catctactac atctgtaagt ccatctccag aaggattaat aaaatttgaa
    12241 aaagatattg atttttttaa ggataagatt atattaagac ctcaagaaat tagtaacaat
    12301 ccagaaatag ccagaagaat tggtgtaatt gcaatgaaca ctgcaattga agttgatatt
    12361 tatggaaatg taaattctac tcacataatg gggtcaaaga tgatgaatgg aattggtggt
    12421 tctggtgatt ttgctagaaa tggagcaata actatattta gtactgagtc aattgcaaaa
    12481 aatggtgata tatcatcaat agtacctatg gtatctcacg tagaccacac tgaacatgat
    12541 gtaatggtaa ttgtaacaga acaaggatat gctgatttaa gaggtcttgc acctagagaa
    12601 agagctataa aaataataga aaattgtgcc catccagatt ataaagaaca acttagggat
    12661 tatttaaata gagcttgtaa aagtggagca aaacaaacac ctcacatatt ggacgaagct
    12721 ttatcatggc atagtaaatt tatgagtaca ggtacaatga aaaaggctga aacttttaaa
    12781 agtgcattat aaaatattta ataaattgga ggtagatttt aaatggaaaa agcagtggaa
    12841 aactttgaag atttaagtaa ggaatacatt aatggttata ttgagagagc aagaaaagct
    12901 caaagagagt ttgaatgtta tactcaagaa caagtagata aaatagtaaa aatagttgga
    12961 aaagtagtgt attataatgc tgaatattta gctaaacttg ctgtagaaga gactggaatg
    13021 ggagtatatg aagataaggt agctaaaaat aaaagcaaag caaaagttat atataacaat
    13081 ttaaaagata aaaaatcagt tggtataatt gatatagata gagaaactgg aataacaaag
    13141 gtagctaaac cagttggagt agtagcggca ataactcctt gtacaaatcc aatcgttaca
    13201 cctatgagca atgcaatgtt tgcacttaag ggaagaaatg caattattat cacacctcat
    13261 cataaagcaa ttggatgtag tacaaaaact gtagagatga ttaatgaaga attagaaaaa
    13321 ataggagctc cagaaaattt aatacaaata cttgaccaac aatcaagaga aaatacaaga
    13381 aatttaattt catcagcaga tgtagtaatt gccactggag gaatgggaat ggtaaaagct
    13441 gcgtactcaa gtggaaaacc agcacttggt gtaggggcag gaaatgttca atgtatcata
    13501 gatagagatg tagacattaa agaagcagta cctaaaatca tagcaggaag aatttttgat
    13561 aatggtatca tttgttcagg tgaacagtca gtaatagtag cagaagaaat gtttgataaa
    13621 ataatggatg aatttaaaaa taataaaggt tttatagtta gagataaagt acaaaaagaa
    13681 gctttcagaa atgctatgtt tgtaaataaa tctatgaata aagatgcagt aggtcaatca
    13741 gttcatacaa ttgcaaaaat tgcaggtgtt gagataccag aagatacaaa aataatagta
    13801 atagaagctg atggaccagg agaagaggat attattgcaa aagaaaaaat gtgtccagtt
    13861 atatctgcat acaagtataa aagttttgaa gaaggtgtag ctattgcaaa agcaaattta
    13921 aatgtagaag gaaaaggaca tagtgtatct atacattcaa atacagttaa aaatatagaa
    13981 tatgcaggag aaaatataga agtatcaaga tttgttatta atcaatgttg tgctactagt
    14041 gctggaggaa gtttctttaa tggtcttgca cctactaata ctctaggttg tggttcttgg
    14101 ggaaataaca gcatctctga aaatcttgat tataagcatc taattaatat ttctagaatt
    14161 gcttattata tgccagaaaa tgaagtccct acagatgaag aactttgggg ataaaattta
    14221 gacaaaaaat attcattcta aattaaagta ggaatatatt ttaatttaga atgaatttaa
    14281 gacataaatt aaatttaaat tagtttattg gactaaattt aaaatattaa gagacatata
    14341 gaagaaggaa gtattataat ttaaaataaa taaaaaaatc tataaaagag aaggagtggt
    14401 tttattatgg cattaatgac aggagctcaa tatattgaaa gtttgagaaa attaaatact
    14461 aaggtttata tgtttggtga agaggttaag aattgggttg accatcctat gattagacca
    14521 tctataaatt gtgtagcagc aacttatgac ttagctcatg acccagaata tgcagattta
    14581 atgacagtta cttcaaatat cacaggtgaa aaaataaata gatttggtca tcttcatcaa
    14641 agtgttgatg atttgataaa aaaagtaaaa atgcaaagac tatgtggtca aaaaacagct
    14701 tcttgtttcc aaagatgtgt tggtatggat gcgtttaatg cagtgtattc aacaacattt
    14761 gagtgtgata aagcccatgg aactaattat catgataatt ttgtaaaata cttaacttat
    14821 atacaagaaa acgatttagt agttgatgga gctatgactg accctaaggg agatagaagt
    14881 ctatcaccta gtgctcaacc tgacccagat atgttccttc atatagtaga aagaagagaa
    14941 gatgggataa ttgtaagagg agcaaaggca catcaaactg gttcaataaa ttcacatgag
    15001 caccttataa tgcctactat aagtatgact gaagcagata aagactacgc tgtatcattt
    15061 gcagttcctt cagatgctga aggagtgttt atgatttatg gaagacaatc ttgtgacact
    15121 agaaaattag aagaaggtgc agatgttgat ttaggaaata aggagtttgg aggtcaagaa
    15181 gctttagtag tatttgataa tgtatttgtt ccaaatgata gaattttctt atgtggagaa
    15241 tgggatttct ctggtatgtt agtagagaga tttgcaggat accatagaca aagttatggt
    15301 ggatgtaaag taggagtagg tgatgtaata ataggagcag cagctttagc agctgattat
    15361 aatggagcaa acaaagcttc acatataaaa gataaactaa tagaaatgac tcaccttaat
    15421 gaaagcttat attgctgtgg tatagcatgt tcatcagaag gtcacaaaac agaagctgga
    15481 aactatcaaa tagatttact tcttgcaaat gtgtgtaaac aaaatgtaac tagatttcct
    15541 tatgaaatag taagacttgc agaagatata gctggagggc ttatggttac tatgccttca
    15601 gaaaaagact ttaaatcaga ccttaaagtt ggaacaagtg gaatgactat tggcgaagta
    15661 tgtaacaagt attttaaagc ttcttcagtg gcttcaacag aagaaagaat gagaatatta
    15721 agattcttag aaaatatatg ccttggttca tctgctgttg gttatagaac tgagtcaatg
    15781 catggggcag gttcaccaca agctcaaaga ataatgattt caagacaagg aaacataaat
    15841 caaaagaaag aattagctaa gaaaattgcg ggaataaaaa aagaggaagc tttaaattta
    15901 taaaaagttg atatataaag ggaaattata tattaatgtg tattaattaa tgaaaaaata
    15961 tgtagtataa gagatagtta taaaattatg gtggttttta gctaagtaaa gaaattttta
    16021 ataaggagaa tttacacatg acctgtggag ttagattttg tgggggctgt aaccctagat
    16081 acgaaagagg gaaggctctt acaataatca aagagcactt caatggtagg gtagatttta
    16141 gtatagcaca agagaacgtc gagtatgatt tgctacttat aattggagga tgcgcaactc
    16201 tatgcgcatc ctatcaacaa tataaagtga aaaatgaatg tgttaagatg tgggatttta
    16261 gccatataga aagagttata aatgtgattg aatcagcaat ttgtgaaact taaaaaagct
    16321 aaatgggggg atttcattga gttggcaaga attgtatcaa agtaaattat gtagtgctac
    16381 agaagctgta aaacagatta aaaacggtga tactgtagta tttgcacatt gtgtaggtga
    16441 acctcctgca ctagtagagg ctatgattga aaatgctgaa caatataaag atgttgagat
    16501 taaacatatg gtaagcttgg gtagtggtgg atatactgct aagggaatgg aagctcattt
    16561 tagagtaaat ccaatgtttg taagtggaaa tgtaagaaag gcaattgaaa atggagatgg
    16621 tgattttaca cctgcattct tccatgaagt accaaagttg cttagagaaa aaagattaaa
    16681 atgtgatgtt gttttagcac aggtaactcc accagatgaa catgggtatt gtagcttagg
    16741 aacatcagtt gattatactt atgaagccat taaaactgct agaacagtaa ttgttcaagt
    16801 gaatgaccag tttcctagaa cctatggtga ggtagtccat gtatcagagt ttgactatat
    16861 agtggaaaaa tcacaacctt tatttgaact acaacctgca aagattggag aagtagaaga
    16921 agcaattggt aaaaattgtg cttcattaat tgaagatggt tctactttac agctagggat
    16981 tggtgggata ccagatgcag ttatgttatt tttaactgat aaaaaggatt tagggattca
    17041 ttcagaaatg atttcagatg ggactttggc cctttatgaa aaaggtgtta taaatggtaa
    17101 atataaaaat tttgataaag aaaaaatgac agttaccttc ttaatgggta ctaaaaaatt
    17161 atatgacttt gcaaataata accctgcagt agaggtaaaa ccagtagact atgtgaatca
    17221 tccagcaatt ataatgaaac aacataagat ggtttctata aattctgcta tacaagttga
    17281 tttaatggga caggtagttg cagaggctat gggacttaga caatttagtg gtgttggagg
    17341 tcaagttgac tttatacgtg gagtgtctat gggtgaagat ggaaaggcaa ttatagctat
    17401 gccttcaata actacgaaaa aagatggtac agtaatttca aaaatcgtgt ctattgtgga
    17461 tgaaggtgca cctataacga catcaagaaa tgatgttgac tatattgtaa cagaatacgg
    17521 tatagcagaa ttaaaaggaa aatctcttag agaaagagcg agaaatttaa taaatatagc
    17581 tcatccaagt gtaagagaaa gtctagcagt agaatttgaa aagagattta aggagaaata
    17641 ttaataatag aggggtgtta ataatggaag cacttagagt agttccaaag atattttact
    17701 ttgatacatt taaagaattt aatgaagaat ttaaaatagg aaaaaatgat ttagtaatta
    17761 ctaatgagtt tatatatgag ccatatatga aacctcttgg aatagataca aatttaattt
    17821 ttcaagaaaa gtttggaaca ggtgaaccat ctgatgaaat gatagactcc atgacaaagg
    17881 aaatgaagaa atacaatttt gatagaatta ttgcatttgg aggaggaaca atagttgata
    17941 tatgtaagat attagcactt gatgttccag aaaagtctat tgatttattt gaaggagaag
    18001 tatcacctaa aaaagtaaaa gagttagtag tagttcctac aacatgtggt acaggtagtg
    18061 aagtaacaaa tgtagctatt gcagagttaa aatctaaaca tactaaaaag gggcttgcag
    18121 tagaagaaac atatgcagac tatgcagtgt taattccaga gacagtaaaa gggttacctt
    18181 acaaattctt tgttacaagt tcggtagatg ctcttataca tgctattgag tcttatcttt
    18241 ctccaaaagc aagtccattt acagagatgt attctctaca agcaataaaa atgattatgg
    18301 atgggtataa gaagatagtt gataaaggag aagaagagag atttaatcat cttagagact
    18361 ttgtacttgc atcaaattat gcaggaattg cttttggaaa tgctggttgt gcagcagttc
    18421 atgcactatc ttattcaata ggtggagctt tccatgttgc acatggtgaa gcaaattatc
    18481 agttctttac tgaagtattt aagatgtatt ctagaaaaaa acctaatgga aaaattataa
    18541 aatgtacaaa aattttagca gatgcccttg aatgtgaccc taactgtgat gtttatggag
    18601 aattagaaaa gtttttaaat aaattaatag ctaaaaaagc tttgagagaa tatggaatgg
    18661 tggaaagtca gatagatgaa tttacagata gtacaatagc aaatcaacaa cgactacttg
    18721 ctaataatta tgtagagctt agtagagagg aaataagaga gatatttgca aatctttatt
    18781 aatacaattt tttatttata tgtatagtca acataaacaa tgctaattat atttatgtac
    18841 aaataaaaat aagggaactt aaattttaat taaagttaag ttctcttatt tttatatgga
    18901 gttaaatata agtatatttt taatttaaaa tatgcttata tttaagtcta ttactttatt
    18961 tgaaacaatt tgtagaattt caaattcctt gacagtaata agtaagtaat tgtataatca
    19021 aactgcaata tattaataat attagtgaaa atgaaaggat tataaaatat gtataaatca
    19081 gaaaattttt caaaaaaatc tgtattaata aattcatttt taagtaaatt tttaaaaata
    19141 attggatggg gtttggtaag cttatttaca ttatcagcct tagtactttc aactatttcc
    19201 attataattg gatgggctgt aatacttcct attcaagtat gtgcagtatt tttacttata
    19261 ggaggagttt gtatttttaa aggtaagaga ttaggagacc aaatttctcg ttataaaaaa
    19321 tattgtacaa ttataaacaa tagaaatatt attccagtag aacttattgc agaaaacaca
    19381 tctaaatcat taaattttat aataaaagat gtacaaaaga tgatagacaa aaactacttt
    19441 ataaatactt atattgataa aagaaataat cagattgtgt taacaaatga agattttgtt
    19501 ccaccaagat atgaagatgt agcatatgaa gtcaaagaag aagaaaagac tgatgaatcc
    19561 aatgaagttg atacaattat aaataaagga atgaattatc taacacaaat aaaagaagct
    19621 aataaaaata ttaaatcaga atcaatgggc gataaaattg tacaagtaga agatgttaca
    19681 agtaaaatat ttgatgttgt aaaacatgac ccatcaaaac ttactcagat acaaaaattt
    19741 atggattatt atcttccaac cactttaaaa ctattaaatt catatcatac attggaagaa
    19801 caaggaattg atagagaaaa tataacaaca actatggaaa gtattgaaaa tacaatgaat
    19861 acgattgtag tagcttttga aaatcaattg gactatctgt ttgaagatga agccattgat
    19921 atttctacag atattactgt tttagagaat atgttagtcc aagaaggatt aacgagtggt
    19981 aaattttaac ttcaatagaa aaaatattct gttattttta aacaattaat tagtcataaa
    20041 gtataaaatg tgtattatat aagttaatca gtactttatg aaggaagagg agagaaagat
    20101 acaatgaata atttagatga tataccagtt atgccaactt tgacacttga cccatttgga
    20161 gaagaatcaa acactaatga tatagataat tctgacttat taatgaaaaa ggatgaaaaa
    20221 gacccagaag aagaaaaact gtctgaatct gaacgcaaga tggtaaagga atttgcggaa
    20281 aaaatagata ttacaaatac aaatatgatt ttgcaatatg gagctggagc tcaaaaaaag
    20341 gtagcaagtt tttctgaaac agctttaaag agtgtaaaaa caagagattt aggagaagtt
    20401 ggagatatgc ttacaaatct agttactgat ttaaaagcat tttcagcaga tgaaaaagaa
    20461 caaagtggtt ttttaggaat ttttaaaaag gcaaataata aaatttcaaa tttaaaaact
    20521 aagtatgata gtgcagaggt aaatgtggat aaagtaagca aagagcttca aaagcatcag
    20581 gttaaaatat tgaaagatat agcaatgctt gataagatgt atgaattgaa cttagcttac
    20641 tcaaaagagc ttactatgta tattatagca ggaaaacaaa aattaaagga tatgaaagaa
    20701 tatgaaatgc caaagttgag agaaaaagct cgtttatctg gttcaacaga agatgctcaa
    20761 tctgtaaatg atatggtttc actttgtgac agatttgaaa agaagattca tgacttagaa
    20821 ttaactagaa tggtatcgct tcaaatggct cctcagataa gattggttca aaatagtaat
    20881 aatctgatgg cagaaaagat tcaatcaact atagtaaata ccattccatt atggaaaaat
    20941 caaatagtgc ttgctcttgg aatttctcac ttaaatcaag ctatgaaagc acagcgtgaa
    21001 gttagtaata tgacaaatga gcttttatta aagaatgcag aaactttgaa gatgggaaca
    21061 atagaaacag caaaagaatc agaaagagga attgtagata tagacacaat taagaaaact
    21121 aatcaatctc taatatctac tattgatgag gtagttaaaa tacaacatga tggtagaata
    21181 aagagacaag aagcagaagt tgaattatca aaaatagaaa atgaacttaa aagtaagctt
    21241 cttgaattta cagttaaata aaaattatac taatataaaa agagactgtc tcaaaataga
    21301 gataagtctc tttttcattg tttaaatgat taaagtatat ttatattata aatttctatt
    21361 atcttttaac ataacatcat gagctccacc ttcaactata ctagtagaag atacaagtgt
    21421 aagtttagca tttttttgaa gttcaggaat gctaagtgct ccacagttac acatagtaga
    21481 acgaacttta cttaaagaaa gagttacatt gtctttcaaa gaaccagcat aaggcacata
    21541 agagtcaaca ccttcttcaa aagaaagttt cttatctcca ccaaggtcat atctttgcca
    21601 gtttctagca cgagcagaac cttctcccca atactctttc atataactac catttatatt
    21661 aactttgtta gttggactct catcaaaacg agagaagtat ctaccaagca ttataaaatc
    21721 tgcacccata gctaaagcta aagttatatg atggtcataa acgataccac catcagagca
    21781 aattggaata taaatacctg ttttttcaaa gtattcgtct ctagcttgag caacttcaat
    21841 tatagaagta gcttgtccac gtcctatacc tttttgttca cgagttatac atatagaacc
    21901 accaccaata ccaactttaa cgaagtcagc accagcttca gctaggtaaa ggaatccttc
    21961 gcggtcaact acatttccag ctcctacctt aactgtatca ccatattttt cacgaatgaa
    22021 atcaagtgtt attttttgcc actcagaaaa accttctgaa gaatctatac ataaaacatc
    22081 agcaccagct tcaactaaag caggaacacg ttcagcaaag tctctagtat taataccagc
    22141 tccaactaca tatctcttag aagaatctaa aagttcaagt ggattttctt tatgagaaga
    22201 gtaatcttta cggaatacca tatacataag attaccatta tcatcaagta caggaagaga
    22261 attaagtttg tggtcccata taatattgtt tgcttccttt aaagtaacat ctttgttagc
    22321 ataaactata gatgacatag gagtcataaa ttcactaact ttagtatcta aatccatacg
    22381 acttatacga taatcacgac ttgcaacaat acccaataat ttaccagttg aagttccatc
    22441 ttcagtaaca gctactgtag aatgtccagt tttttctttt aagtcaagta tgtcttttaa
    22501 tgtgttatca ggtttaatat ttgaatcact tacaacaaaa ccagctttgt gggatttaac
    22561 acgtgctacc atagcagctt cattttcaat agtttgagaa ccgtatataa atgaaatacc
    22621 accttcttta gcaagtgcaa tagccatttt gtcatcagaa acagattgca taatagcaga
    22681 agtcaaaggt atattcatat aaatatctgc ttcctcgccc tttttaaatt tagtaacagg
    22741 tgtttttaaa cttacattag caggtataca atcagtagaa gagaaacctg gtacaagaag
    22801 gtattcactg aaagtatgtg atggtgtatc aaaataaaac gccatttttt aatcactcct
    22861 tatattttta tactttttat aattgatagt attaattata acttattctt taattttatt
    22921 aaagtatttt gtcaaatatt gagatttttg aaaaatttaa actattttaa ttaaatttgt
    22981 ttaatatata ttgccttata gggtaaagtt gtatattaaa taatgtttag tgtttaatag
    23041 tagctaattt aaaattaatc tttagtgatt catataatta catttgtata gtttacattg
    23101 ataatattac tagagagtgt ataaacctca aaaatacaga taaattaaat ctatattagt
    23161 atatccaaaa gtacatttaa taaatttatt taactgaatt attattttca tttctattaa
    23221 atttaaatat tttataaa
//
LOCUS       DAGSRM010000057        23222 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.057, whole genome shotgun sequence.
ACCESSION   DAGSRM010000057 DAGSRM010000000
VERSION     DAGSRM010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23222
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.057"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            402..1238
                     /locus_tag="MJE33_002366"
     CDS             402..1238
                     /locus_tag="MJE33_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBY9648298.1"
                     /translation="MNIKKIFNNNVVVSSLEDGTEIIVTGAGVGFKKKVGDLIDENLI
                     SKKYFVQDDQRDKYNQILNKTSIEYFKISEEIIEKANEVLNTQVNDSIILALTSHIEF
                     AVQREKQGIKLPNLILNETKQLYREEFEFGLWAIDEIEKRIGIKLPEDEAGYIAIHII
                     NGLENSQKDEGINILEFSKQVIQIIEEVHGFKLDVNSLNYTRLITHLKFFVQRILRKE
                     TYNDSDIEDMYKLVNKNYNKPKVCTEEIAALVLDRFEYKISKEEELYLMIHINKITNS
                     GC"
     gene            1591..3168
                     /locus_tag="MJE33_002367"
     CDS             1591..3168
                     /locus_tag="MJE33_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBY9648299.1"
                     /translation="MKKNKLGLWNFFQMLGKTFMFPIALLSVSGMMLGLGAGFTDPKM
                     IEMVPFLGNEYVNLVLNFMLTIGLFAFNNLGALFAMAIPLGLLKKEKEFGAFSGLVGF
                     IAMHIGTNFYLTRADLLVPAEQMSTNGQAMIMGIQTYNTSVLGGIVAGLIVYAIYDKV
                     SNLKIPESLGFYSGPRLVPIVSLIVMSIVGLLIPIVWPPFFNAFQSLGRWISSAGPLG
                     YFSYAVAERVTIPFGLNHLVTSVFRFTPIGGTAVIDGETYFGTLNMFMAYVENNQIIP
                     LDLAGKMEQGKLMIQYGLAGAALAIYRTAKPENRKALKGLLISGVLTVIIGGISEPIE
                     FLFLFISPALFAFHTFMNGLANMVLPYLGVLMGFTGDLIAFISFGVLRGTATGWPIAV
                     LVAVLYFAIYYFVFKWAIIKFNIKTPGREDKEIKVSEGNGSNFKNLSTYKGQQMISAL
                     GGQSNILSLDNCVTRLRLKLQDVSLINEDAIKEAGGIAVVKLDEHTIQVIIGTQVYSL
                     RKQMDKAMEGHLEACND"
     gene            3161..4345
                     /locus_tag="MJE33_002368"
     CDS             3161..4345
                     /locus_tag="MJE33_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HBY9648300.1"
                     /translation="MINFDKKVDRLGTYCTQWDYVEDRFGKKDLLPFTISDMDLEFPF
                     EITEVLQERLNHRVLGYSRWKHDDFRNSVTNWYIKRFNCSVDSNWIYYSPSVMYSISK
                     LIEMLSNEGDGILINTPAYNAFYDVIINNNRNIIKSHLVNRNGFYSIDFNDFEKKCKK
                     SKIFILCNPHNPTGRVWSELELVKMIGICKNNDVKIISDDIHMDIVYDNRMTPILNVA
                     KDYLDSVFICTSASKSFNIPALTGSYVIIPNEGIRNKFENITRYRDFVNSPAILAILA
                     TMVCYDDCEYWLDELLKYLKENLKYTIDYIKDNLEPLKLVMPEGCYFAWIDFSELKIS
                     GEKFQKLLMDIGEVAIMSGSVYGEEGEFYLRLNVACSREKLEDGLNRIKKTMDYIEKR
                     IL"
     gene            4363..4962
                     /locus_tag="MJE33_002369"
     CDS             4363..4962
                     /locus_tag="MJE33_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBY9648301.1"
                     /translation="MGYLKTMVDVMTTEMNAIRCLIDEAGIEYESIVSEIANCEGKVI
                     FMGVGKSAHIGKKLAATFASTGTPSFFVHATEAVHGDLGMIESKDITILISNSGNSME
                     VVNCIKYIKAIGSKTIAFTSNRNSVLAKECDYALIYPAKDEADHLNLAPTTSSTITLV
                     LGDSIACALSKSSNFGSSDFYKYHPGGSLGEKLKTANNG"
     gene            4986..5456
                     /locus_tag="MJE33_002370"
     CDS             4986..5456
                     /locus_tag="MJE33_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBY9648302.1"
                     /translation="MFKMFKKKSIKSPISGKVIELSKIQDGVFSQKIMGEGVAIDSTG
                     DIVYAPINGKIVVVAETKHAFVIELENGMELLIHVGLDTVNLKGEGFEVLVSMGEQVK
                     EGTPMLKINRPLIESKGISLITPVTITNHSEYNMNTCNVGNSVEGGKDTVIEFK"
     gene            5815..6561
                     /locus_tag="MJE33_002371"
     CDS             5815..6561
                     /locus_tag="MJE33_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBY9648303.1"
                     /translation="MKIENLSIDDIKRGYIFNDDLNCYSCIKCTKTYEVGEIYKIGNR
                     FFEASKAIELHMELEHSDYLEQLLNSDSKYNTLTENQKQLFQLFISDLPDKEIAKQVG
                     VSTSTIRHQKFIFREKAKQAKFYLALYESVFEDKSNKSSTIIPIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKKYSEKEVNQILES
                     IYDDFATIRRYLIEYGFMSRNKECTKYWLT"
     gene            6622..7296
                     /locus_tag="MJE33_002372"
     CDS             6622..7296
                     /locus_tag="MJE33_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HBY9648304.1"
                     /translation="MNDELKSILYNKGVDIVRFVDISEFPINQTQGFSKAILFCIGLS
                     KKFITDIYNNLPTDSDEFLEKEEKVEELADWISKYIQNKGYRAYSQSEKNNLEHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RYPLIDSKCMDCNVCVENCPAKAIHGNEWTLPGKRESIIDVSKCFCVLRCMMSCPWSL
                     RYANQK"
     gene            7665..9011
                     /locus_tag="MJE33_002373"
     CDS             7665..9011
                     /locus_tag="MJE33_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBY9648305.1"
                     /translation="MEIETKTPDWINVLELNSKIDITGKYDVSNMIQNIVSDKSLEGS
                     IIYFPKGNYLFEKGIKISQQITLQGDSYYGRGNQVSNLDKKQSIIGTTNFITRGVSNM
                     SIITLSGTSQCIKNINFYYDSHDIEKIPPKNVSAITEYGETQGLSHFEHLFISGFSGI
                     GIEIPYYSTGNDITVSSCGLGMRLGEKSMLSSSKIYECKNGMEITTGVSLNNVRIEEI
                     KEIGINNKGFGFNLIMNLMVDQCGYCGFMFEKMCYSQITARITRCGQYYKSVDYNAYE
                     NMEDRKEEAYSIFYGDILENCNIVLMNRNIDTLESDSLNEHKIYVIKANETNNVMLTC
                     YAEADNFIQCAKGNLLLENGENTYKFYNGEICSVGGVQISDIDEKDLIKIIENTLYID
                     DKGKYLMVPRLKKNTVIYSVLDSTEEINKLHGGIWEQIDVKVLAGESMYYYKKIKD"
     gene            9038..10438
                     /locus_tag="MJE33_002374"
     CDS             9038..10438
                     /locus_tag="MJE33_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648306.1"
                     /translation="MEESIIIKDISGWINVLDYGICNDETKDVSFKLQEIMDAVPEDS
                     IIYFPKGKYLFESGVKIKKRLTLCGDSYILANVPKNTAGVTQFTFKGKKNPKSENRAI
                     ITAKSVRHCIKNICFYSDSCDRDMTSNHEPPTDGKPRFHHNVIINYRNISAVICEDKQ
                     GEPGHYENLSFSGFSGIALDMPNNSTANDITIFTSGLGISTGENSTIANSKVWGCVDG
                     MIISTGTFINGMRVEEIKKVGIKNIGRGLNFITNITIDQCGYCGVWFDSISNVQISGN
                     ITRCGQYYYDVSYDNYLMLDNRVKEAYSLFYGNLLESSNIVLSNTNKDNWEDFKEDKH
                     KVYLVEALETKNVLLTCNIDATDWIVKSEKGNLTYNNMRQTVIFYDGKLSSINGIGIS
                     DEDYGDKVKIKKGNIYINSKDKDIKMHKPEVGDIMSTIIKDKSILSAHYGGTWEKIGE
                     KIEFGITVHYYKLIEK"
     gene            10648..12084
                     /locus_tag="MJE33_002375"
     CDS             10648..12084
                     /locus_tag="MJE33_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBY9648307.1"
                     /translation="MEGRTGIKDGRWINVVERGIKNDGSYNIGPALQDIIDEIETDFC
                     VLYFPKGKYLFTSGVKINKEIVLQGDSNSSTGTTQFITRGVENMSIITLTGKKQCIKN
                     INFYSDSCEIQVNKEPPTKGNPKYHYEMIINKNEKNVEMNNVSAILYDNSKNMIKGLG
                     HYENIYISGFSGTGIRIPYYSIVNDITVSSCGLGIDTGIDTIISNSRISKCQNGMRIR
                     TGTSINNVRIEKIQKVGLISSQVKDYEGYGSYKINNITIDQCGYCGFLFDSVKGSYFS
                     GIIRRCGQYYYNTDYDTYLSIKDRVEEAYSLFYGNYFTECNIDLVSDNKDNWDDGLEY
                     KHKVYVFKTLQIPKLTLRCNIDAYDYIVKSVHGGGNLMLKNNFNNYIFIDSSNPEVIN
                     GINFNYQKNGNLLRLNNGKLSYKNGKEETAIFRPNYGLIVASTDSECEQIETSYGGKW
                     EKLGSEVIGKETVYYYKHHEPDKTNQIQ"
     gene            12428..13372
                     /locus_tag="MJE33_002376"
     CDS             12428..13372
                     /locus_tag="MJE33_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HBY9648308.1"
                     /translation="MIVSVSRRTDIPAFYSKWFFNRLKEGFVYVINPMNPKQVSKIEL
                     NPHTVDCFVFWTKDVTPMLYDLNKLKDYKYYFHYTITSYGKEVETCILDKRKVIDSFK
                     ELSRKIGKERVILRYDPIFLSEKYTVDYHIKAFASLCNQLDGFTEKCIISFIDLYKKT
                     KFNTKALHIKPIEITEIETLSKEISKIAHKHGIILEMCSEEYNLSKFGIGKSKCIDDR
                     LISKIIGCEVEVKKDSTQRDICGCVKSVDIGQYNTCRHYCLYCYANFNYAKVEENCRL
                     YKEDNKLLIDTLRDDAKISIRDMKTIKVDKNKQISIFD"
     gene            13541..14473
                     /locus_tag="MJE33_002377"
     CDS             13541..14473
                     /locus_tag="MJE33_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBY9648309.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGFIGFILY
                     AIYIVKIILLRKGVKEQLENPLISSTFPTITMATMIFATYIKPLSLELSIMIWSIGIV
                     GHLVLIVLFSKKFLVKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVILKRVWIIKEMPEPSLPSIAILAAPGSLLLSGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLMNGYLTKSGHFISWISY
                     ASKLEEIIATVIILYVLVLFIIYLSKSETTSKVK"
     gene            14578..15045
                     /locus_tag="MJE33_002378"
     CDS             14578..15045
                     /locus_tag="MJE33_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C_GCAxxG_C_C family protein"
                     /protein_id="HBY9648310.1"
                     /translation="MKTKEEYLKEVRNISEEYFRKGEFFCSEAVLQTINDALGQPLSP
                     EITKLASGFPIGLGKAQCLCGAISGGEMALGIVYGRVHGEAMNPKMFEHAKGLHDYIK
                     KEYGATCCRAITKKWDGDNFMSPERKEHCIKITGQVTEWVADKLIEDEKLNIK"
     gene            complement(15289..16143)
                     /locus_tag="MJE33_002379"
     CDS             complement(15289..16143)
                     /locus_tag="MJE33_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009912760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar uptake protein"
                     /protein_id="HBY9648311.1"
                     /translation="MLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSISAFIFST
                     IITLVIHPEITFFSFCISMISGIFWSLGQLMQFKAIQIASVSKVMPISNGSQLTFTTL
                     LAVILFNEWTSSKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVLLSSLFL
                     SLYVVTNQIFLIEGYRIILPQSVGMLITSSIIVKYFSKEIVYMENVLFNLITGILWSI
                     ANLGMFITTNTLGVTISFSISQSCVIVATLGGIIFFKEKKNKKEWLAIFIGIILIMSG
                     VFMLSIIK"
     gene            16309..18273
                     /locus_tag="MJE33_002380"
     CDS             16309..18273
                     /locus_tag="MJE33_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBY9648312.1"
                     /translation="MLTDMIQNREIKIIKFLLKEGHTTVKAIADDLDVSEKTVSKSLK
                     EVEAVLKKIGLSLIRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVLLK
                     SNDYITIQQLSDELYISRGTIERDLAEVEKLLKNEGISIYKKPSKGIKLNISERDKRQ
                     LTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIDILRNFSRERK
                     FEFSDYEFQSLVIHLAIAVERIRKGEYIEESLYTGNIENNQMSNTNYLAKQLEEKFSI
                     LLPKSEIGYINLHLVASDQHRDTNELKNTSTNPCETSTNNISNLREIIKNTLYETAYD
                     KDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDIRI
                     NDDECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIEKV
                     LSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSSYTSKK
                     YKSFRNLIFEENILLNIEASNYEDAIKYITDNLIKKGFAKEGIAKSAIERENLSYTSF
                     QSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEVFY
                     DFIDNKKNINDILSATSTKEVYDLLVEESI"
     gene            18273..18734
                     /locus_tag="MJE33_002381"
     CDS             18273..18734
                     /locus_tag="MJE33_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY9648313.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSNAVKKSSIIFAKTKHKIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALKLANSEQEIINIFSEEGEM"
     gene            18737..19057
                     /locus_tag="MJE33_002382"
     CDS             18737..19057
                     /locus_tag="MJE33_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBY9648314.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            19073..20173
                     /locus_tag="MJE33_002383"
     CDS             19073..20173
                     /locus_tag="MJE33_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBY9648315.1"
                     /translation="MLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGSFYNIP
                     DIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPGLVGGM
                     IAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLITVVLIN
                     YVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTSLALLT
                     SGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSEGAIPF
                     AVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSIAVGTV
                     IVAGLVIVLRGNPVIEDDEFEDDDFDEAEWES"
     gene            20293..22893
                     /locus_tag="MJE33_002384"
     CDS             20293..22893
                     /locus_tag="MJE33_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBY9648316.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLHKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKQVQSPYFK
                     WKSLNGESYIYAVNMPHGYGTGMLLEPSLKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKIKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARIH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLINTWKKLLEGQAHD
                     SLAGCVTDTVTDDILHRIREANEICDSIENTIVKKISEGLKLSKNDILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDATIIEETYVKPRENVLEETPAGNIFIEEPGYYILKVR
                     INIQLPALGYKVVSFELSDKEMVSLDKSNDTFISNDNYKIIYEDGSLNLQLKDGTYIS
                     NFLTLKDSGNAGDTYDFSPLKNDEDIKLSFDKVYTEKALGYEKMVVKGTTLLPLTLED
                     RKNKYLKGKLNVKVSIVLSKENPLMDVKLCVDNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRKNGVLDNWEDIYSEMPIDLEPLESNITLTNQNRSCTVFTRGIKEYQHIEDEI
                     ALTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQMLKELEFSFAIYMDSKS
                     FDELEIANLTHNYLRECVNYQLQDYNFFLYRIDNKIQKSIVNKTVSRELEIISLPENY
                     LISACYPSYYSKDKFIIRVENPSSNRMILDEQIFKNRNGKIVNAIEDVEEEQVYEIAP
                     FDVISILLDL"
BASE COUNT         9153 a         2372 c         4076 g         7621 t
ORIGIN      
        1 tataaaaaaa tttaatttat ataaattaaa aattatatta ccaatgtata tatttgagaa
       61 aagattagta caaagctata atctgaattt caataaatat atatataatt aagttgaaac
      121 acttgaaaat actggaaaaa attgaaaata gtttttgtaa tataaaatgt cgattgattt
      181 taaataatta aatatacata agaaataatt aaatatattt caatgaaaat atgataaaat
      241 atgacaaatt aattgatgac aataaaaaat attttgttat tgtagtatta tagggtaaat
      301 tgcaaaatat atacgaaagt aaaattttaa atattgacat taaattaaat atactatact
      361 atgtaagtgt gatgagagaa tttttttaga ggggatttat agtgaatata aaaaagatat
      421 ttaataacaa tgtagtagta agctccctag aagatggcac agaaataatt gtcactggtg
      481 ctggagtagg atttaagaaa aaagtaggcg atttgataga tgaaaatttg atttctaaga
      541 aatattttgt tcaagacgat caaagggaca aatacaatca aatacttaat aagacctcta
      601 ttgaatactt taaaatttca gaagaaatta tagaaaaggc taatgaagtt ttaaatactc
      661 aagtgaatga ttcaataata ttagccttaa ctagtcacat agaatttgct gtccaaagag
      721 aaaaacaagg tataaaatta ccaaacttaa tattgaatga gacaaaacaa ctttataggg
      781 aagaatttga atttggatta tgggcaattg atgaaattga aaaaaggata ggaataaaat
      841 tgcctgaaga tgaagcagga tatattgcta ttcacataat caatggtttg gaaaattctc
      901 aaaaagatga aggtattaat atactagagt tttcaaaaca agttattcag ataattgaag
      961 aagtacatgg atttaaattg gatgtaaatt cattaaatta tactagactt ataactcact
     1021 taaagttttt tgttcaaagg attctccgaa aagaaactta caatgatagt gatattgaag
     1081 atatgtataa gttagtgaat aaaaattaca ataaacctaa agtgtgtact gaagaaattg
     1141 cagcattggt attagacaga tttgaatata agattagtaa agaagaagaa ttatatttaa
     1201 tgattcacat aaataaaata acaaacagtg gttgttagga ttgtaactga taaagcaggc
     1261 aaaacctaag tcattgagct taactattag attaagtttt ttgatttagg tttttttatt
     1321 agatacaatc aaaatacaga cagtgacttt taggattgta actggttaag caggcaaaac
     1381 ctaaatcatt gaatttaact attaggttaa gttttttgat ttaggttttt ttattagata
     1441 caatcaaata cagacagtgg ttgttaggat tgtaactggt tgagcaggca aaacctaaat
     1501 cattaaactt aattattaaa ttaagttttt tgatttaggt ttttctataa atttgattat
     1561 gtttttagat aaaaatagga ggggaaaatt atgaaaaaaa ataagttagg tttatggaac
     1621 ttcttccaaa tgttaggaaa aacgtttatg ttcccaatag cactacttag tgtttctggg
     1681 atgatgctag gacttggggc aggttttaca gaccctaaga tgattgaaat ggttccattt
     1741 ttagggaatg aatatgttaa cttagtttta aacttcatgt taacaatagg attatttgca
     1801 tttaacaact tgggagcatt atttgcaatg gcaattccac taggattatt aaaaaaagaa
     1861 aaagagtttg gtgctttttc aggattagta ggatttatag caatgcatat aggtacaaat
     1921 ttttatttaa caagagctga tttgttagta ccagcagaac agatgtcaac taatggtcaa
     1981 gctatgatta tgggtataca aacatataat acaagtgttt taggtggtat tgtagcagga
     2041 ttaattgttt atgcaatata tgataaagtt agtaatttaa agataccaga atcattagga
     2101 ttctacagtg gaccaagatt agttccaatt gttagtttaa tagttatgag tatagttgga
     2161 ttattaatac ctattgtttg gccaccattt ttcaatgcat tccaaagttt aggaagatgg
     2221 atttcttcag caggaccatt aggatatttt tcatatgcag tggcagaaag agttacaatt
     2281 ccatttggac taaatcatct tgtaacatct gtattccgtt ttacaccaat aggtggaact
     2341 gctgttattg atggagaaac gtactttggt acattgaaca tgtttatggc atatgtagaa
     2401 aataatcaga ttatacctct tgatttagct ggtaaaatgg aacaaggtaa attgatgatt
     2461 caatatggat tagctggtgc tgcattggct atctaccgta cagcaaaacc agaaaataga
     2521 aaggcactta aaggactttt aatatcagga gtgttgacag ttattatagg tggtataagt
     2581 gaaccaatag agtttctatt cttattcata tcaccagcat tatttgcttt ccatacattt
     2641 atgaatggtc tagcaaacat ggtacttcca tacttaggcg tgttgatggg attcacagga
     2701 gatttaattg catttataag ttttggtgtg ttaagaggaa cagctacagg atggccaata
     2761 gcagtattag tagcagtttt atattttgca atatactatt ttgtatttaa gtgggcaatt
     2821 attaagttta atataaagac cccaggacgt gaggataaag aaataaaagt atctgaagga
     2881 aatggttcaa atttcaaaaa tttgtctaca tataaaggac aacaaatgat atctgcttta
     2941 ggtggtcaat ctaatatatt atcactagat aattgtgtaa ctagattacg tttaaaatta
     3001 caagatgtat cattaataaa tgaggatgca attaaagagg ctggtggtat agcagttgtt
     3061 aaattggatg agcatacaat acaagtaatt ataggaaccc aagtgtattc tttaagaaag
     3121 cagatggata aagctatgga aggtcatttg gaggcatgta atgattaatt ttgataaaaa
     3181 agtagaccga ttgggtacat attgtaccca gtgggactat gtagaagata gatttggtaa
     3241 gaaggatttg cttcctttta caatttcaga tatggattta gagtttccat ttgaaataac
     3301 agaggtgttg caggaaaggt taaatcatag agttttggga tacagtagat ggaaacatga
     3361 tgattttaga aattcagtta cgaattggta tattaaaaga tttaattgta gcgttgatag
     3421 taactggata tattatagtc caagtgtaat gtattcaatt agcaaactta ttgagatgtt
     3481 aagtaatgaa ggagatggaa tccttataaa tactcctgca tacaatgctt tttatgatgt
     3541 gataataaat aacaatcgta atattataaa gtcacatctt gtaaatagga atggattcta
     3601 ttcaattgat tttaatgatt ttgagaaaaa gtgtaaaaaa tctaagatat ttattttatg
     3661 taatcctcac aatccaactg gtagagtatg gagtgagtta gaacttgtta agatgatagg
     3721 aatttgtaag aataatgatg tcaagataat atcagatgat atacacatgg atattgtata
     3781 tgacaataga atgacaccta tcttaaatgt tgcaaaggac tatttagatt ctgtattcat
     3841 ttgtacatca gcgtctaaat ctttcaatat accagcattg actgggtcat atgtgattat
     3901 accaaatgaa ggtattagaa ataaatttga aaatatcact agataccgtg attttgtaaa
     3961 ttcgccagca attctagcaa tattagccac aatggtctgt tatgatgact gcgaatattg
     4021 gttagatgaa ctattaaaat accttaaaga gaatcttaaa tatacgattg attatataaa
     4081 agataattta gaaccattga aattggtcat gccagaaggc tgttactttg cttggataga
     4141 tttttccgaa cttaaaataa gtggtgagaa attccaaaaa ttattgatgg atattggaga
     4201 agttgctatt atgtcggggt ctgtatatgg tgaagaaggt gagttttatt taaggttaaa
     4261 tgtggcatgt tcaagagaaa aacttgaaga tggtttaaat aggattaaaa aaactatgga
     4321 ttatattgaa aaaagaattt tatagtagga agggagatag ttatgggata tttaaaaaca
     4381 atggttgatg ttatgactac tgaaatgaat gccataagat gtttaataga tgaagcagga
     4441 atagaatatg aaagtatagt tagtgaaatt gcaaattgtg aaggcaaagt aatttttatg
     4501 ggtgtaggaa aatctgcaca tataggtaag aaattggcag ctacatttgc tagtactggt
     4561 acacctagct tttttgtaca tgcaacagag gcagtgcatg gagatttagg aatgattgaa
     4621 agtaaggata ttacaatttt aatatcaaat agtggaaatt ctatggaagt ggtaaactgt
     4681 ataaaatata ttaaagcaat aggttctaag acaattgcat ttacttcaaa cagaaattca
     4741 gtactggcaa aagagtgtga ttatgcttta atatatcctg caaaggatga agcagaccat
     4801 ttaaatctag caccaacaac atcatctaca attactttgg ttttaggaga ttctattgca
     4861 tgtgcattat ctaagagtag taattttggt tcttcagatt tttataagta ccatccagga
     4921 ggaagtttag gtgagaagtt aaaaacagct aacaatggat aaaagcaaaa aggagagtag
     4981 tttttatgtt caaaatgttt aaaaaaaaat caataaaatc acccatatca ggaaaagtaa
     5041 tagagttatc taaaatacaa gatggtgtat tttctcaaaa aattatggga gagggtgtag
     5101 ctatagattc tacaggagac attgtatatg ctccaatcaa tggaaagata gtagtagtag
     5161 cagagactaa acatgcattt gtaatagaat tagaaaatgg gatggaactt cttattcatg
     5221 ttggtttaga tactgtaaat ttaaaaggcg aaggttttga ggtattggtg agtatgggag
     5281 aacaagtgaa ggaaggaaca ccaatgttga aaattaaccg tcctttgatt gaatcaaagg
     5341 gtatatcact tattactcca gttacaataa ctaatcattc agagtacaat atgaatacat
     5401 gtaatgtagg caatagtgtt gaaggtggga aagatactgt aattgagttt aaataaagaa
     5461 ctaaaataca aggttaatat ttataaatac ctataaactt atgttttata agattgattg
     5521 tatagatttg ttgtataaaa aagaactctt taattataaa ttaaggagtt cttttttatt
     5581 ttgtaaatat tgtttagatt tatatagaaa aacacttagt aataaataga ataaataagt
     5641 atttatatag ctaaaaatct aaatagtttt gtttaaatat atttttatat tttgtaattt
     5701 caaattaata aataattaga tttgataaac aaaaaataaa aatgttgatt acattaataa
     5761 actttaatat aatatattta tgaaaaatgt aaactgtgaa atggaggtag taacatgaaa
     5821 attgaaaatc tttctattga tgatattaaa cgaggctata tatttaatga tgatttaaat
     5881 tgttatagtt gcattaaatg tacaaaaaca tatgaggtag gagaaatata taaaataggt
     5941 aatagattct ttgaagcttc aaaagctatt gaacttcata tggaattaga acatagtgat
     6001 tatttagagc aattgcttaa tagtgattca aagtacaaca cattgactga aaatcaaaaa
     6061 caattatttc aattatttat tagtgattta ccagataagg agatagcaaa acaggttgga
     6121 gtatccacat ctactatacg ccatcaaaaa tttattttca gagaaaaagc aaaacaggct
     6181 aagttctatt tagcactata tgaatctgta tttgaagata aatctaataa aagtagtact
     6241 ataataccaa tccatgaaca tgctcgaatg gtagatgaaa gatatgtagt aactgaagaa
     6301 gagagaaaac acattttgga tacatcattt gaaagtatga atcctttaaa attaaaagta
     6361 ttttcttcaa aagaaaagaa aaaggtcgta attttatcta agattttgga acaatttgaa
     6421 cgtgggaaga aatattcaga aaaggaagtt aatcagattt tagaatctat atatgatgat
     6481 tttgcaacaa ttagaagata tctaattgag tatggtttta tgagtagaaa taaagaatgt
     6541 acaaaatatt ggttaacata attaaaataa taaattttac taatgtgtaa tgttattttt
     6601 atctaggggg aggagtcaaa tatgaatgat gaattaaaat ctattttata caataaaggt
     6661 gtagacattg ttcgctttgt tgatatatct gaatttccaa taaatcaaac acaaggtttt
     6721 tctaaagcaa ttttattttg tatagggtta tcaaaaaaat ttattacaga tatatacaat
     6781 aacttaccta cagatagtga tgagtttttg gaaaaagagg agaaagtaga agaattagct
     6841 gactggattt caaaatatat tcaaaataaa ggatatagag cgtattctca atcggagaaa
     6901 aacaatctag aacatggata ttttgaaaaa gcctatatta atccagaaat gcaatcaggg
     6961 attagtcctt taccacataa gacaatagca aatattagtg gcattggatt tatgggaaaa
     7021 aataatttgt ttgtaacaga ggaatatgga tgtgctttta gcatgtgtac agtacttaca
     7081 gatgctccaa tttctgttga aagatatcct ttgattgatt caaaatgtat ggattgcaat
     7141 gtttgtgtag agaactgtcc tgcaaaagct atacatggaa atgaatggac tttacctgga
     7201 aaaagagaat ctattattga tgtttcaaag tgtttttgtg tattgaggtg tatgatgagt
     7261 tgcccatggt cattaagata tgcaaatcaa aaataacttt actagagaag gtattaaatt
     7321 gaatattata aaatttttaa aatgtaagat tatataagat ttttcaaaat aaaaatttgt
     7381 atagaacact cagaaatgag tgtttttcta ttgggattaa attatattga tacaaagctt
     7441 aactatttta atcattatat aaaatttgta ttaaaaaatt aagaataact tggtaatagt
     7501 gaaaatataa tgagattata attgatttat aatataacta taatgatatt gtaaaaatga
     7561 aaattgtcct ctcaaaattc ccaaagagag taaaagtcac ttcagttata gtcaaaaaaa
     7621 tttttctgtg aaaagaattt agatttgaaa aggaggaatt aatcatggaa attgaaacta
     7681 aaactccaga ttggattaat gtactagaat taaacagtaa aatagatatt actgggaaat
     7741 atgatgtaag taatatgata caaaatattg tctctgataa gtcactagaa ggttccatta
     7801 tatattttcc aaaaggtaat tacttatttg agaaaggtat taaaattagt caacaaataa
     7861 cacttcaagg agattcttat tatggaagag gtaatcaagt atcaaattta gataaaaaac
     7921 aatcaatcat aggaacaacc aattttataa cccgaggagt ttctaatatg agtattatta
     7981 cattgtcagg aacaagtcaa tgtatcaaaa atattaattt ttattatgat agtcatgata
     8041 tagagaaaat accgcctaaa aatgtatctg ctattacaga atatggagaa acacaaggac
     8101 tatctcattt tgaacatctg tttatttctg gattttcagg gataggaatt gaaattccat
     8161 actattcaac tggtaatgat attactgtta gttcatgtgg tcttggtatg aggcttggag
     8221 agaagtcaat gctttctagt agcaagattt acgaatgtaa aaatggaatg gagattacta
     8281 ctggtgttag cttaaataat gtgagaatag aagaaataaa agaaataggt attaataata
     8341 aaggatttgg ttttaacctg attatgaatc ttatggttga tcaatgtgga tattgtggat
     8401 ttatgtttga aaaaatgtgc tatagccaga taactgccag aattactaga tgcggtcagt
     8461 attataaaag tgttgattat aatgcttatg agaatatgga agataggaaa gaagaagctt
     8521 attccatttt ttatggagat attttagaaa attgcaatat tgtattgatg aatagaaata
     8581 ttgatacctt agaaagtgat tcacttaatg aacataagat atatgtaatc aaggcaaatg
     8641 aaacaaacaa tgtaatgcta acatgttatg ctgaagcaga taactttata caatgtgcaa
     8701 agggcaactt gctgttagaa aatggtgaaa atacttataa attttataat ggtgagattt
     8761 gttctgtagg aggtgttcaa atttctgata tagatgaaaa agatttaatc aaaataatag
     8821 agaacacttt gtatatagat gataaaggta agtatttaat ggttcctaga ttaaaaaaga
     8881 atactgtaat atattcagta ctagattcta cagaagaaat aaataaatta catggtggaa
     8941 tatgggaaca gattgatgta aaagtattag ctggggaatc catgtactat tataaaaaga
     9001 taaaagatta aattatcaaa ctaggaggta gtgattaatg gaagagtcta taataattaa
     9061 agatataagt ggctggatta atgtacttga ttatggaatt tgtaatgatg aaacaaaaga
     9121 tgtaagtttt aagttgcaag agattatgga tgctgtacca gaagattcta ttatatattt
     9181 tccaaaagga aaatacttat ttgaaagtgg tgttaagatt aagaaacgat taaccttatg
     9241 tggagattct tatattttag cgaatgttcc taaaaatact gctggagtaa cacagtttac
     9301 ttttaaagga aagaaaaatc ctaaaagtga aaatagagca atcataacag caaaaagtgt
     9361 aagacactgt attaagaaca tttgctttta ttcagatagt tgtgatagag atatgacatc
     9421 taatcatgaa cctccaacag atggaaaacc tagatttcat cataatgtaa ttataaatta
     9481 tagaaatata tctgctgtta tatgtgaaga taaacaggga gaaccaggtc attatgaaaa
     9541 tctatctttt agtggttttt ctggaattgc acttgatatg cctaataatt ctacagcaaa
     9601 tgatattaca atttttacat ccggacttgg aattagtaca ggggaaaatt caacaattgc
     9661 taacagtaag gtttggggct gtgttgatgg aatgataatt tctactggaa cttttataaa
     9721 tggtatgagg gtagaagaaa taaaaaaagt tggaattaaa aatattggta gaggtttaaa
     9781 ttttattaca aatatcacaa ttgaccaatg tggatattgt ggagtttggt ttgattctat
     9841 atccaatgtt cagatatcag gaaatatcac aaggtgtggg cagtactatt atgatgtaag
     9901 ctatgataat tatttaatgt tggataatag agttaaagaa gcatattctt tattctatgg
     9961 taatttattg gaatcaagta atatagtttt atcaaatacc aataaagata attgggaaga
    10021 tttcaaagaa gataagcata aggtgtatct tgtagaagca cttgaaacca agaatgtact
    10081 attaacatgc aatatagatg ctactgactg gattgttaag tcagaaaaag gaaatttaac
    10141 gtacaataat atgcgacaaa ctgttatatt ttacgatgga aaactaagta gtatcaatgg
    10201 aattggtata tctgatgaag attatgggga taaagtcaag ataaaaaaag gaaatatata
    10261 tataaatagt aaggataagg atattaagat gcataaacca gaggtaggtg acattatgtc
    10321 aacaattatt aaagataaaa gtattctttc tgctcattat gggggaacat gggagaaaat
    10381 tggtgaaaaa atagagtttg gaataactgt acactattat aaattgatag agaaataagt
    10441 aaatacgtag tatttgaata gatgtatttt attggagagc taggactata gttttaatgt
    10501 aaatggtttg gtaatagcat ggattgaata atataaaaat aatataaaaa attaatttga
    10561 ttatgtaaaa ttctatgaag tagtaaaact gtgactttag tcataggagg ttcaaactaa
    10621 gtaaaaggga aaggaatata taatattatg gaaggaagaa ctgggattaa agatggaaga
    10681 tggattaatg tagtagaaag aggtattaaa aatgatggtt cgtacaacat aggtcctgct
    10741 ttgcaggata ttattgatga aatagaaaca gatttttgtg tcttatattt tccaaaaggt
    10801 aaatacctat ttacaagtgg tgttaaaatt aataaagaaa ttgtattgca aggtgattct
    10861 aattcatcta caggtactac acaatttata actcgtggag tagaaaatat gagtattatt
    10921 acattgacag ggaagaaaca atgtattaaa aatataaatt tctattctga tagttgtgag
    10981 atacaggtaa ataaagaacc accgacaaaa ggtaatccta aatatcatta tgagatgatt
    11041 atcaataaga atgaaaagaa tgttgagatg aacaatgtat cagcaatcct gtatgataac
    11101 agtaagaata tgattaaagg tctaggccat tatgaaaata tttatataag tggtttttcg
    11161 ggtacaggaa ttaggatacc atattactca attgtaaatg atattacagt ttcatcttgt
    11221 gggctgggta ttgatacagg aatagataca attatttcta atagtagaat atctaagtgt
    11281 caaaatggaa tgaggattag gactggaact agcataaata atgtaagaat tgaaaaaata
    11341 cagaaagttg gtcttataag tagtcaagtt aaggattatg agggatatgg aagttataaa
    11401 attaataata ttacgattga ccaatgtggg tattgtggtt ttttatttga ctcagtgaaa
    11461 ggtagttatt tttctggtat tataagaaga tgtgggcagt actattataa tacagattat
    11521 gacacatatt taagtattaa agatagagta gaagaagcat attctttatt ttatggaaat
    11581 tattttacag aatgtaatat tgatttagtt agtgataaca aggataactg ggatgatggc
    11641 ttagaatata aacataaagt ttatgtattt aaaacccttc aaataccaaa attaacattg
    11701 agatgcaata tagatgccta tgattatatc gttaaatcgg tacatggagg aggtaattta
    11761 atgttaaaaa ataactttaa caattatata tttattgatt catctaatcc agaagttatt
    11821 aatggaatta attttaatta tcaaaagaat ggaaacttgt taaggttaaa taatggtaaa
    11881 ttaagttata aaaatggaaa ggaagaaaca gctatattca gacctaatta tggtcttatt
    11941 gtagcatcaa cagattcaga atgtgaacaa atagagacta gctatggagg aaaatgggag
    12001 aagcttggtt ctgaagtaat tggaaaagaa acggtatatt attacaaaca tcatgaacca
    12061 gataaaacaa accaaataca ataaaatttg tattatataa tgatataaag ttttaatata
    12121 tagtgtttat ctttaatgta atattggtat atttgtatag tattaaattt gttaaaaagt
    12181 aaaattgtag ggtgcttgaa aatagcacct tttttagttt cattataaaa ttagttttga
    12241 aagtttagga ataatgtata tttattttta aagaattata accttattaa ggatatttta
    12301 aatgctagaa atgaatcagt aaatcatact ggaattatct taaatgtttt gattaaatta
    12361 agtatttaga gctataatta tattaaaaat atattagaac ataattatat tactatggag
    12421 gttaaatatg atagtaagtg tgagtagaag aactgatata cctgctttct atagtaagtg
    12481 gttttttaac agacttaaag aaggttttgt ttatgtaatt aatccaatga atccaaaaca
    12541 ggttagtaag atagagttaa atccacatac tgttgattgt tttgtatttt ggacaaagga
    12601 tgtaactcca atgttatatg atttaaataa attaaaagac tataaatatt attttcacta
    12661 tactataaca tcatatggaa aagaagtaga gacatgtatt ttagataaaa gaaaggtaat
    12721 agattcattt aaggaattgt ctagaaaaat aggcaaagaa agagtaattt taagatacga
    12781 cccgattttc ttaagtgaaa agtacacagt agattatcat atcaaagcat ttgcgagttt
    12841 atgtaatcag cttgatggat ttacagaaaa atgtataata agttttatcg atttgtataa
    12901 gaaaacaaaa tttaatacta aagcattaca tataaagcca atagaaatta cagaaataga
    12961 aacattatca aaagagattt ctaagattgc tcataagcat gggataatac ttgaaatgtg
    13021 ttctgaagaa tataatttaa gtaaatttgg gattggaaaa agtaagtgta ttgatgatag
    13081 attaatatca aaaattatag gatgtgaagt agaggtaaaa aaagatagta cacaaagaga
    13141 tatttgtgga tgtgttaaaa gtgtcgatat aggacaatac aatacttgca gacattattg
    13201 tctgtattgt tatgctaact ttaattatgc aaaagtagaa gaaaactgta ggctttataa
    13261 ggaagataat aaattactca tagatacttt aagagatgat gcaaaaataa gcattagaga
    13321 tatgaagact attaaagtag ataagaataa acaaattagt atatttgatt aaaataaata
    13381 tactaattac cttaatatat taagaatata gatacttttg ataataaaat taaacttaat
    13441 agatattttc tatataagaa agatggtcat aatatgtcaa tttgaccatc tttttgatat
    13501 aattaaaaca caaataattt agtaacaaag gaggcagaac ttgaatttat taaaaaaata
    13561 tcctatacct attgcaagtt tgattttgtc aatctttact ttaggtaact tattacaatc
    13621 ttatagtgaa tcattacgta atgtaattgg atttatagga tttattttgt atgcaattta
    13681 tatagtcaaa ataatattac ttagaaaggg tgttaaagag caattagaga accctttaat
    13741 atcaagtaca tttccaacta taacaatggc aacaatgata tttgctacat atataaaacc
    13801 attatcttta gaattaagta ttatgatttg gagcatagga attgtgggtc atcttgtgtt
    13861 aatagtctta tttagtaaga agttcttagt taaattctca ataaaaacgg tatttccatc
    13921 ttggtatata gtatatgttg ggatagttgc agctagtgtt acggcaccag catttaatca
    13981 acttttgatt ggcaaaattg ctttttggat aggatttgta tcttatatag ttcttatacc
    14041 agtaatttta aagagagtat ggataatcaa ggaaatgcct gaaccatctt taccaagtat
    14101 agctatatta gctgcaccag ggtcactatt attgtctgga tatattaatt ctttttcaga
    14161 taaaaataat gtagtattat acttgttatt catattatca attgtatttt atattatagt
    14221 tttgggatac ttaccgaaat tattaagatt gcctttttat ccgagttttg ctgcatttac
    14281 attccctatg gcaataagtg caatgggtat gaaactaatg aatggttatt taactaaatc
    14341 aggtcatttt ataagttgga tttcatatgc ttctaagtta gaagagataa ttgctacagt
    14401 gattatttta tatgttttag ttttgtttat tatatatctt agtaaatctg aaactacatc
    14461 taaagttaaa taagaattat taaatatgtg tatttaatag gaatattgat ataaatatat
    14521 gaagataaga taaaccatag aaacttaatt tagcttaatt ttattggagg aaaacaaatg
    14581 aaaacaaaag aagaatattt aaaagaagtt agaaacatat cagaagaata ctttagaaaa
    14641 ggagagtttt tttgctctga agcagttctc cagacaataa atgatgcttt aggccaacct
    14701 ttaagtccag agataactaa gttagcatca ggatttccga taggacttgg aaaggcacaa
    14761 tgtctttgcg gagcaatatc tgggggagag atggctttag gtatagtata tggaagagtt
    14821 catggtgaag ctatgaatcc aaagatgttt gagcatgcta aaggtttgca tgattatatt
    14881 aaaaaagagt atggtgcaac atgttgtaga gctattacaa agaagtggga tggtgataac
    14941 tttatgtctc ccgaaagaaa agagcactgt attaaaataa caggtcaagt tacagagtgg
    15001 gttgcagata agcttataga agatgaaaaa cttaatatca aatagtgcaa tattagttgg
    15061 tgaatttata aagttaaatt tacttatagt ataagatact tgatattctc gtattgatag
    15121 aaaaatttat agcatatgta tttaatgaaa tatacttata tttagagtgt ggtttaattt
    15181 cataaataca tatgctatta ttttaagtat ataagcaaat ttatttaaat aaatagaaac
    15241 agttaattta tatgtacaaa actatatatg attagtgtaa ataaatacct attttataat
    15301 actcaacata aagacaccag acatgattag tattatacca ataaaaatag caagccattc
    15361 ttttttgttc ttcttttctt taaaaaatat aatccctcct aaagtggcta ctatcacaca
    15421 actctgagag atagaaaaac ttattgtaac tcccaatgta ttagtcgtta tgaacatacc
    15481 taaatttgct attgaccaca atataccagt aattaaattg aataaaacat tttccatata
    15541 tacaatctct ttagaaaaat acttgacaat aatagatgaa gttatcaaca taccaacaga
    15601 ttgaggtaat ataattctat atccttcaat taaaaatatt tgattagtta caacatataa
    15661 tgataaaaat agagatgata ataatacaac tccaacacat tttaacatat ttttatctgt
    15721 agatttttta gtttgataat ttgtgagaag tataccaagt attatacata atattgataa
    15781 agaaccaatc agtaacattt tagaactggt ccattcatta aacaagatta cagctaataa
    15841 tgtcgtaaaa gttaattgtg aaccatttga aattggcatt actttagata cactagcaat
    15901 ttgaattgct ttaaactgca ttaattgtcc cagactccag aatatgccag aaatcatact
    15961 tatacaaaat gaaaaaaatg taatttcagg atgtataacc agagttatta tagtggaaaa
    16021 tataaacgca cttatggatg tacctaaaag ctgttcatga ggagaacttt tctttagatt
    16081 tgcaactatg ggcatcaatc cccatcccat tacaggaagt aaagcaataa ttattgtcag
    16141 cattaaacta tttcctttca tattaaattt agacagcttt taatttatca tctaaaatta
    16201 aagtgttcaa tacataaaaa ttaccgtatt taatcaggaa atattagtag ttttttagaa
    16261 aaacattttc cattaaaata aagacaaagg gaaggagtaa aattgaaaat gttaactgat
    16321 atgattcaaa atcgtgaaat taaaataata aagtttttat taaaagaagg tcatacaact
    16381 gtaaaagcaa tagcagatga tttagatgtt tcagaaaaaa cagtttcaaa atctcttaaa
    16441 gaagtagaag cagtgcttaa aaagataggt ctttctttaa taagaaaacc taagataggt
    16501 gtttatattg atggagattt aaaaaaaata cttccttacc tagataataa aggaagtcaa
    16561 attcctacaa ccagagagga gagagttatc tatatatatt cagtactttt aaagagtaat
    16621 gattatatta ctatacaaca actttctgat gaactatata taagtcgtgg aactattgaa
    16681 agggatttag cagaagttga aaaactgcta aaaaacgaag ggatttccat atataagaaa
    16741 cctagtaagg gaataaagtt aaatataagt gaaagagata aaagacaact tactgctaag
    16801 tttatacata aattttggag tagaaactgg tatattaagc aagagcaaga aagcttttat
    16861 caagcatttg aaaagataca agcagatgta aatggtattt tttcggaaga tgatttatca
    16921 ataattattg atattcttag gaatttcagc agagaaagaa aatttgagtt ctctgattat
    16981 gaatttcagt cattagttat tcatttggca atagctgtag agagaattag aaaaggcgaa
    17041 tatatagaag aaagtttgta tactggtaat atagaaaata atcaaatgtc aaataccaat
    17101 tatttagcaa aacaattaga agaaaaattt tcaatattat taccaaaatc tgaaattgga
    17161 tatataaatt tacatttagt agcgtctgac caacatagag atactaatga actaaaaaat
    17221 acttcaacta atccttgtga gacaagtaca aataatatat caaatcttcg ggaaattata
    17281 aaaaatacat tgtatgagac agcttatgac aaggatttaa ttgatggatt aacttcacat
    17341 gtacattcat caataaatag actgaaatat ggacttagta ttaaaaatcc atatgtaaat
    17401 acaatcaaac aaaacttttc tttatcattt gagcaagctt tagatttaaa gaaagtaatt
    17461 gaatttatat atgatattag gataaatgat gatgaatgtg cttatatagc attacatttt
    17521 gaagctttta gagaaagaat gaaagaactt ccacaaaata ttagagtaat tctagtatgc
    17581 agtacaggat taggttcatc taggttattg tctgcaagaa taaaaaaata ctttccaatg
    17641 ataaagattg aaaaagtact ttccgtacaa gaattagtat ccagtaaaat tgatgcagat
    17701 ttagtaataa gtactattta tttagagtta gaaacaattc caaccatagt agttagtcct
    17761 attttaaatg aaagtgatat aagaaccttg gaaagaaata tacaaaatgt ttcaagttat
    17821 acttctaaga agtataaatc tttcagaaac ttaatctttg aagaaaatat attgttgaat
    17881 attgaagctt caaactatga agatgcaatt aagtatataa cagataacct aataaaaaaa
    17941 ggttttgcaa aagaaggtat agctaaaagt gcaattgaga gagaaaattt atcttacact
    18001 tctttccaat caatagctac acctcatgct aatcctagtt atattaaaaa atctaatata
    18061 tctgttgcaa cgttaaaaaa tcctattaca tggggaaatg aaaaggtgga agttattttc
    18121 tttatttcat tagaaaatga caaaaaccta gagcttgatg aaatatatga agttttttat
    18181 gacttcattg acaataaaaa aaatataaat gacatattaa gtgcaacttc cacaaaggaa
    18241 gtgtacgatt tgctagtgga ggagagtata tagtgaaaat atcagatgta ttgaaaaaag
    18301 agcaaattgt ttttaatctt gatacaaata caaaggaaga aacaataaaa aaattaagta
    18361 gtatatttgt tgaaaatggg attgtggaca atgaagaaga atatgttaag tctgttttgg
    18421 aaagagaaga acattcaact acaggtattg gtaatggtat tgctattcct catggaaaaa
    18481 gtaatgctgt caaaaaatct tctataatat ttgcaaagac taagcataag attgaatggg
    18541 atgctttaga tgaaaaacca gtagatagta ttttcttatt ggctataagc aatattgata
    18601 aggatagcaa tcatcttgta ctattatcta aattagcaac taaacttatg gatgatgaca
    18661 atgtagatgc actaaaatta gccaattcag aacaagaaat aatcaatata tttagtgaag
    18721 aaggagagat gtagaaatga aaagaaaaat tatagcagtt acagcatgtg caacaggagt
    18781 tgcacataca tatatggcag ctcaagcgtt aaagaaagct tcaaaaaaga tgggtcacct
    18841 tataaaagtt gaaacacaag gagctacagg aatcgataat gagttaacta caaaggatgc
    18901 acaaattgca gaagtagtaa tatttgcagt agatactaaa gtcagaaatg ctgaacgttt
    18961 tgagggaaaa gaaatactag aagtaccagt taatgcacct ataaaggatg cagagaaagt
    19021 aataaatgat gcactaaagt taattgacac taaataaatg gagggaaata taatgttgaa
    19081 aaagatagtc tctgagttaa aaaagcatgt cttaactgga atctcatata tgattccatt
    19141 agtaattgca ggtgctatga taatggcgat atctcgtgta ggtggttctt tctataatat
    19201 accagatata tgggatgcta aatatgcaga aagtgctagt agtattgtta gattattaca
    19261 tgatttagat gggtttggag gtactgcatt aggattaatg tttcctgtaa ttgctgcatt
    19321 tataggattt tctatagctg ataaattagc tattgtacca ggtcttgttg gaggtatgat
    19381 tgctaaagat ataggtgcag gatttttagg agctttggca gttggtctta tagcaggata
    19441 tacatgtttg tttattaaaa atcatgtcaa attaccaaaa tcagcagctt ctatagtacc
    19501 aatatttata gtaccagtat ttgggacatt aatcactgtt gtattaatca attatgtgat
    19561 aggtatacct tttgccacat taaatactgg attagaaaat tggttaaatg gattatctgg
    19621 tacaaatcag atattaatgg cagctattat aggggctatg atggcagttg acttaggtgg
    19681 accagttaat aaagcagctt taacaacatc acttgcatta ttaacaagtg gaatttatgc
    19741 gcctaacact gctgcaatgg taggaatagt tataccacca ctaggattgg gacttgctac
    19801 tatactagca aaacaaaaat ataataaaca gttacgtgaa gctgggaaat catctcttat
    19861 aatgggttta attggagtaa gtgaaggagc aattcctttt gcagtagaat ctccattaaa
    19921 agtaatacct tcatgtgtaa ttggaacagc aattgcttca gctatggcag ttggtttagg
    19981 ttcagtaaat gcaactccaa ttagtggatt ttatggatgg tttacagtag agaattggcc
    20041 aatgtatgtg ttatcaatag cagttggaac agtaattgta gctggattag ttatagtatt
    20101 gcgtggaaat ccagtgattg aggatgatga atttgaagat gatgattttg atgaagcaga
    20161 gtgggaatct taaaatttaa taactattta agacgtgtta taagctatat tcaatatgaa
    20221 tcttatacta aataaagtgt taaatattca gtttagactt aaatataaaa gataaagaac
    20281 agaggagaat aaatgataaa agcacatatt gttaatcata cacactggga tagagaatgg
    20341 tattttactt ctggagatgc attagtactt agtgaacaac tatttacaga tgtaattgat
    20401 gaattagaac gtaatccaga tgtaagtttt gtacttgatg gacaactttc tattttggat
    20461 gattatgtac aattacacaa agaaaaaata gaaatcatta aaaagttaat aaaagaagat
    20521 agactacata ttggaccatg gtttactcaa actgatgcat tctttgctag aggagagtct
    20581 attttaagaa acttaatgat tggaattttt gaaagtaaaa agtatggtaa gtatatgaaa
    20641 attgggtatt tgccagatac atttggattt aatgcacaga tgcctatact attaaagcat
    20701 gtaggtcttg ataatatatt cttttggaga ggtatacatc ttggtaaaca ggttcaatca
    20761 ccatatttta agtggaaaag cttaaatgga gaaagttata tatatgcagt aaatatgcca
    20821 catggatatg gaactggtat gcttttagag ccttcattaa agtatgttaa tggacgctta
    20881 gacccagcta ttgattttat taaatcatat actgatgtag atgaggtgtt aataccttct
    20941 gggaatgacc agttaaatat aataggtgat ttcaaaaata aaattaaagc gattaatgat
    21001 atcggaaagt attcatatat aacgagttca tatcaagagt ttttaaaata tataaagtcc
    21061 atagaatcac tagaaagcta tagaggtgaa tttagagaac ctgtattagc aagaatacat
    21121 aaatcaattg gttctagtag aatggatata aaattagcat gtgataaact agaaaacaag
    21181 ctgattaaaa gaatagagcc attattagta attgctaaaa agagtaaaat tgaaataagc
    21241 aatcagttac ttataaatac atggaaaaaa cttttggaag gacaagcaca cgatagttta
    21301 gctggatgtg taactgatac tgtaacagat gatattcttc accgtataag agaagcaaat
    21361 gaaatatgtg acagtattga aaatactatt gtaaaaaaga tttcagaagg gcttaaatta
    21421 tctaaaaatg atattttagt atttaataca gaagctaagc gttttaatgg atataaagag
    21481 atacaagttg taagtgatag taaaaatatt tattttaaag ataatttaga tgctactata
    21541 attgaagaaa cttatgtaaa accgagagaa aatgtattag aagaaacacc tgctggtaat
    21601 atatttattg aagaaccagg atattatata ttaaaagtaa gaataaatat acagcttcct
    21661 gcacttggtt ataaagttgt tagttttgaa ttaagtgata aagaaatggt gtcacttgat
    21721 aaatctaatg acacttttat atctaatgat aattataaga taatatatga agatggaagc
    21781 ttaaatttac aacttaaaga tggaacttac atatcaaact ttttaacatt aaaagatagt
    21841 ggtaatgcag gtgataccta tgacttttct ccactaaaaa atgatgagga tataaaactt
    21901 tcgtttgata aagtgtatac agaaaaagct ttaggatatg aaaaaatggt ggttaaaggt
    21961 actactttac taccacttac attagaagat agaaaaaaca aatatcttaa agggaaatta
    22021 aatgtaaaag taagtattgt cttatctaaa gaaaacccat tgatggatgt aaaactttgt
    22081 gttgataaca cgatatataa tcatagacta cgtgttcata taaaaactga tataaaagat
    22141 aataaaaaca tagcatcatt gccatttgga tatataacaa gaaaaaatgg agttttagac
    22201 aactgggaag atatttacag tgaaatgcct atagatttag aacctttgga aagtaatatt
    22261 actctaacaa accaaaatag aagttgtact gtatttacta gaggaataaa agagtatcaa
    22321 catattgaag atgaaattgc attgacctta cttgctacta cagatgaact aggaaagcct
    22381 gatttattat atagaccagg acgtgcttct ggtgatacaa ctaaaaaagg tcatataaga
    22441 attaaaacag ataaagctca aatgcttaag gaattagaat tttcatttgc aatatatatg
    22501 gattctaaat catttgatga attggaaatt gcgaatttaa cccacaatta tctaagggaa
    22561 tgtgtaaatt atcaattaca ggattacaat ttctttttat atcgtataga taataagatt
    22621 caaaaatcaa ttgtcaataa aactgtaagt agagaattag aaataatatc tttaccagaa
    22681 aactatctta tatcagcatg ttatccttct tattacagta aagataaatt tataataaga
    22741 gtggaaaatc caagtagtaa tagaatgata ttggatgagc aaatatttaa aaatagaaat
    22801 gggaaaattg taaatgcaat agaagatgta gaagaggaac aggtatatga aatagcacct
    22861 tttgacgtta tttcaatatt attggattta taagtgtaaa agtaagttta ttgtttaaag
    22921 tctgacagat attaatctgc cagacttttt aatttgtata aatttgggta aatattaatg
    22981 aatggtttta gtatttaagt atgaatttag tatttaatat agaatttatt gaagtagaat
    23041 aattagttta acaggataag cagtttaaat aaagaattat tatattagag tgattttata
    23101 tataatttaa aataataaaa ttttaataaa atgagttaaa gattgtatat taatagagtt
    23161 gatgtagtta caaaaaagtt accaattgtt atgtatttac aaaactgata tattaattac
    23221 at
//
LOCUS       DAGSRM010000058        22934 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.058, whole genome shotgun sequence.
ACCESSION   DAGSRM010000058 DAGSRM010000000
VERSION     DAGSRM010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22934
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.058"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(63..812)
                     /locus_tag="MJE33_002385"
     CDS             complement(63..812)
                     /locus_tag="MJE33_002385"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBY9648317.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDNELELVCVAARGLDSYEGDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDDEMNKIREAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
     gene            complement(879..1766)
                     /locus_tag="MJE33_002386"
     CDS             complement(879..1766)
                     /locus_tag="MJE33_002386"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBY9648318.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            complement(1883..2884)
                     /locus_tag="MJE33_002387"
     CDS             complement(1883..2884)
                     /locus_tag="MJE33_002387"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBY9648319.1"
                     /translation="MKKVNVAIVGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTGRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRVVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     gene            complement(3309..4190)
                     /locus_tag="MJE33_002388"
     CDS             complement(3309..4190)
                     /locus_tag="MJE33_002388"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBY9648320.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENGTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDGLL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPSVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPKDFAIYSGNDDTILPLLSLGGSGVISVLANICPKETHDL
                     VTKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKQELTNFGFKF"
     gene            4513..5706
                     /locus_tag="MJE33_002389"
     CDS             4513..5706
                     /locus_tag="MJE33_002389"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBY9648321.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            5912..7030
                     /locus_tag="MJE33_002390"
     CDS             5912..7030
                     /locus_tag="MJE33_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HBY9648322.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEVYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNIDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVSSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFSPEVYEKMKLRVRELARGFELAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAIGSDIVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYSKLLKYKEAID"
     gene            7161..7406
                     /locus_tag="MJE33_002391"
     CDS             7161..7406
                     /locus_tag="MJE33_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HBY9648323.1"
                     /translation="MKEKVQREKKITNGKNTKILTNDDIMKYEIASELGLMDKVGERG
                     WAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            7393..8139
                     /locus_tag="MJE33_002392"
     CDS             7393..8139
                     /locus_tag="MJE33_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBY9648324.1"
                     /translation="MSQYSDFAFVYDELMNEVDYNGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLTKKNYDIAGIDISDEMLSVAREKAEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENIFSKTYELLKKDGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENLIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLEK
                     SGFKDIKYYGDFTFDKPSEESQRIFFVCKK"
     gene            8422..9297
                     /gene="hslO"
                     /locus_tag="MJE33_002393"
     CDS             8422..9297
                     /gene="hslO"
                     /locus_tag="MJE33_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBY9648325.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIIATSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPEDILNI
                     VLDGLEPKILDTCEVGFICECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYSEEELLEILKEM"
     gene            9688..10488
                     /locus_tag="MJE33_002394"
     CDS             9688..10488
                     /locus_tag="MJE33_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBY9648326.1"
                     /translation="MESKTKYIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRELGVKPQNVIILADIDKKEGTHYELMEKTILDFEHKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKREGKIKDDLYFVKPKYVKYIPRNERAIYKAMDFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
     gene            11015..13255
                     /locus_tag="MJE33_002395"
     CDS             11015..13255
                     /locus_tag="MJE33_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBY9648327.1"
                     /translation="MNNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNNIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWHWNIFAESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSREGEISEATKKKLSKLLIEDRCPICDGRRLNERVYRSLI
                     NGYNIADLTSMQIDELAEVIKSIDEPEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHIVDVGPKAGKYGGEVVYEGSYENLLTSGTLTGNALSKFLPIKESVR
                     EHSGYLEVKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPSAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNVCEGKKFKKEVLEYRFQGKNIIEVLEMSVLEAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIS
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIELGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
     gene            13669..15204
                     /locus_tag="MJE33_002396"
     CDS             13669..15204
                     /locus_tag="MJE33_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBY9648328.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTIMTITGSILIALLALIFDFILGLVEKFFQRKRKKKNRKKV
                     FAILGSLILIATIFTSYLFNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLNVEI
                     TQGVGGGTSNIQPAMENGEFDIYPEYTGTGWNMVLKKNELYTESMFSKLQSEYKTKLK
                     MQWTGMYGFSNSYALAVRKEVADKYNLKNITDLKDVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVVVLKDDKNFYPSY
                     QCGNVVRLEVLEENPELSKVFKMVGDILTDKEMAKMNYDVENNNLEPREVAREFLNKK
                     GLL"
     gene            15220..15981
                     /locus_tag="MJE33_002397"
     CDS             15220..15981
                     /locus_tag="MJE33_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9648329.1"
                     /translation="MTPIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQTDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKNKLEVKSAVNKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNTPANQFVDQLLNIRKSIIDDDNLKE"
     gene            16319..17188
                     /locus_tag="MJE33_002398"
     CDS             16319..17188
                     /locus_tag="MJE33_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBY9648330.1"
                     /translation="MDNNLITIEKTLDYIEKHLLDEMLDLNVISKEIGYSKYHLHRMF
                     TSVVGFSVHNYIQRRRLTEAARMLIFTEKPIIEIALISGYDTQRSFSRGFKKMFKRSP
                     NFFRRHQDFLPLQLKFDIHNRKKLRGDMVLDVKKVNCADINIVGYSGKVRKGFHIIGK
                     CWRDLHKNKEMIENKVDLGFLIGVSDYSNFKEIENTPTFTYIAGAQVATFGKLPNGME
                     KFVLPKSNYIVFTFIGKNEDSMQNVVEYIYQEWFPNSSCQFNENNLYDFVKYGEKTDE
                     NGLSEIQFWVPII"
     gene            17517..17768
                     /locus_tag="MJE33_002399"
     CDS             17517..17768
                     /locus_tag="MJE33_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HBY9648331.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLETNDVVNWCKTKIE
                     SPNAIINRKGKNWYISVYDCIITVNAHSYTIITAHKEKK"
     gene            17880..18659
                     /locus_tag="MJE33_002400"
     CDS             17880..18659
                     /locus_tag="MJE33_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBY9648332.1"
                     /translation="MNDTVKEKLAESLTSDTTDILPYLPYLLQDFWELGSSPEQMLQL
                     FRKHIPFNEDTKVLDLACGKGAVSVKLAKELNIHIKGIDLIPEFIDYARRKAIEYKVD
                     ALCDFEMNDANSSVKIEHDYDCVIFGAVGDILGNYKETISKLLPTIKSKGFILIDDAY
                     VVDKSTNEQLNFERVYPTYNEWCILFKELGLKLIECIPCLELENDVEEEMNWIITRAN
                     ELIKKYPSQKDMFEQYVISQKNEYTDLQQDLVGATWILQKE"
     gene            complement(18794..19444)
                     /locus_tag="MJE33_002401"
     CDS             complement(18794..19444)
                     /locus_tag="MJE33_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648333.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSELKQLIQNDNKVFTFKVDNTSK
                     YPDALNAILNCDRLIIASPLYVDGIPSHLLHFLEKCEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIEHWCKKINFTWVQGIGIGAGEMFGSLESVPLGKGPKKDLGIALKE
                     LSKNILSDNIHIDDSNKSTIFISPNFPRFLYKLMGNHQWNTQAKSNGLTKSDILKK"
     gene            complement(19441..19944)
                     /locus_tag="MJE33_002402"
     CDS             complement(19441..19944)
                     /locus_tag="MJE33_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBY9648334.1"
                     /translation="MRLLIHDLTDKELEKLLPKLDDNIKILPNTKEIHKCIGCFGCWI
                     KTPSECVVKDYYSNMGKLINDSDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKQINLKAIFYGDCITEAEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            complement(19993..20508)
                     /locus_tag="MJE33_002403"
     CDS             complement(19993..20508)
                     /locus_tag="MJE33_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBY9648335.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGNKLKKLYSITNTGKEVFLEWLKKPINFSKTKQDHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKNDAEENHCVDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            20788..21312
                     /locus_tag="MJE33_002404"
     CDS             20788..21312
                     /locus_tag="MJE33_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBY9648336.1"
                     /translation="MHEQIDCCERKKRTIDIKGENMRELLKWVSIINRYNTNYIDRKL
                     ADIGINNSQYFYVLHICNNPGITQDTIFEKILVNPSNITRALANLEKEGFITREPGVK
                     DKRTWHLYPTEKSLGCYDEILKITNGYVEELLSSFEKEEQELFMSMLKKVAFLAIDMN
                     EEAKLEAKENGRKI"
     gene            21296..22699
                     /locus_tag="MJE33_002405"
     CDS             21296..22699
                     /locus_tag="MJE33_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBY9648337.1"
                     /translation="MEEKYKEPLEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIG
                     QGVGVLGNAATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICL
                     MAVFGISLFIVVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLIL
                     ADGRPKASMFCMLIGAVINTILNPLFIFVFNMGIAGSALATVIGQLISFGISVHYIKN
                     FQHIKLHKKSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSE
                     IPLACVGIIIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIIS
                     VIAFLCFQLFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGK
                     SVLGLFTSLTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSL
                     TIKQNNLMSNHNKIAEV"
BASE COUNT         8546 a         2754 c         3902 g         7732 t
ORIGIN      
        1 cgtattatta tatctatatt gcatatgttt aatttatatt gtgtattatt atatttatac
       61 tattatccca acatgctgtc catattataa aatcctggtt tttgcttaac taagtatttg
      121 gcagcagcta tagaaccact tgcaaagata ccacgagatt gagcttgatg gcttattgta
      181 agaacctcat tgtctccagc aaatagagca tcatgttcgc caacaatatt tccacctctt
      241 atagcatgaa taccaacttc atttggttgt ctttttgcac tacgaccata tctaccataa
      301 ttattttcaa cgtttggcaa aacctctttt atagcgttag caatcataac agctgtgcca
      361 cttggggcat ctacttttct gttgtgatgt ttttctatta tttctatatc aaatccttct
      421 agtaatttag ctgcatcttt aaccaatttt actaatacat ttacaccaag agacatatta
      481 tatgattgga atacaggaat aactttagcc gcttcacgta ttttgttcat ttcatcatca
      541 ttatatccag tagtagcaat tactaaaggt gttttagtct ttaaaacgta agataagata
      601 ttatctaaat tagagtgatg agaaaaatct ataactacat cagcttcctc aactatagta
      661 ctcatatctt cataaatttt aaggtcgcct tcatagctat ccagacctct agctgcaaca
      721 caaaccaatt ctaattcatt atcttctctt atagtatcgg ctaaaatctt acccattttt
      781 ccatcataac cagttgctat aacttttaac ataatttcct cctatgatta agggctaact
      841 tttgaccaaa cttacttttt accatgcatt aatctttact atgcattaac ttttagtcca
      901 aagttaacta attctttctt taatacttct aaatttgctg ggtccatttc tgctagaggt
      961 aatcttaaat ctccaacatt gaatcccaat aaattcattg cagtcttaac tggtatagga
     1021 tttacttcta taaataaagc tgcgattaga gcatccatac caagttgtaa ttttctagaa
     1081 ccttcaatat cgccttcaaa gaatttagct accaaatcgt gagtttcttg agggcaaaca
     1141 ttagcaagta ctgatataac tccttgacct cctaaagata ataaaggaag tatagtatca
     1201 tcattaccag agtagatagc aaagtcatct ggaactaaac tagctatttc tgcaacctgt
     1261 cctaggtctc cactagcttc ttttacagca actatgttat caattttagc aagttcaact
     1321 ataaggctag gacttatatt tacacaagtt cttccaggga cattgtaaag tataattgga
     1381 agtttaacac tgttagctat tgtttcaaaa tgacgcttaa gtccagcttt attagcttta
     1441 ttgtaataag gtgttattat taatagagca tctactccta atttttcagc ttcttgactt
     1501 aagttaactg agtgcatagt atcattagaa ccacttccag ctataacagg aactctttta
     1561 ttaacttttt caacaacata cttaatagta gcaagttgtt cttcatcagt catagtatta
     1621 gcttcaccag tagtaccaca agatactata gcatcagtac cattagctat ttggtattca
     1681 actaattcac ctaatttgtc aaaatcaact ttgttatcct ttgtaaatgg tgtaactaga
     1741 gcaacagcag aacctttaaa tatcatataa aatcaatccc ctctttttta tttttgtgtt
     1801 ttatattaca gttacttcta atgcttgttt ttgagttact attaatattt tacaaaatta
     1861 tatatttata caagtttgta tattatacta aatcgtatga taaagccatt tcagctattt
     1921 gaattgcgtt agttgcagca ccttttctta tattatcagc aacaacccaa agatttacac
     1981 cattatcaac actaaaatct cttcttattc ttccaacaaa tacttcatct ctatcatcaa
     2041 attctacagc agttggatat tcgtttttag caggattatc aactactaca acacctggag
     2101 cttcttcaag tgtttttact aaatcatcta actcaaatgg attgttaaac tcaacattta
     2161 tagattcact atgaccattt tttactggaa ctcttacagt agttgcagtt atttttaaat
     2221 cgtagttatc aagtattttc atagtttcat ttaccatttt catttcttct ttagtgtatc
     2281 cattttcagt gaatgaatct atatgaggaa gacagttgta agagatttga tgagggtaga
     2341 atccagtagg attaccgttc attcctttct ctaaatcttc aacaccttta acaccagaac
     2401 cagatacagc ttggtaagta gagtaaacaa ctctcttaat tttgtattta tcatcaagag
     2461 ctttaagggg aaccatagct tgtatagtag agcagttagg attagctatt atacccttat
     2521 gattttttat ggcttctgga ttaacctcag gaactactaa aggcacatct ggattcattc
     2581 tccattgact agagttatct actactaaga ttccatttga agcagctatt ggagcatact
     2641 tttcactaat acttccacca gcagagaata atgctatttg tatgcctcta ccagtaaatg
     2701 aagtttcctt taattcttct actatatatt ctttaccaca aaacattact ttagaaccag
     2761 ctgatttaga agaagagaat aaaaatagat tttctattgg aaaatttctt tcttctaata
     2821 cttttaaaaa tgttctacct accattccag tagcaccaac tattgctaca tttacttttt
     2881 tcatataaat tcaccccaat tttaaattat taaaacatta ttttatttta gttatacaag
     2941 taagatatgt taatattttt atatatccaa aaactttaat attatattaa caactttaat
     3001 tatatacaaa aaacgtacaa aggtatactt tgtacgtcta atttatcact caaagtaaac
     3061 cctgtagctc atcacctaca aaaataagtg acagtattac acatgttatg tgcaatccca
     3121 gaaaatatta ttgcaagaaa tattttcttc ggctgtaaat cctttttcca atcattaaca
     3181 cctgccagct actattggat actcttagtt acagctacct ctaccctagg ttaataggat
     3241 attaaaattt gattattcta ataataatac atatttaaga aaaaatcaac ttatttatat
     3301 aaatattgct aaaatttaaa tccaaaattt gtcaattctt gttttaacac attaagatta
     3361 gtttcttcca tttcagctag aggaagtctt aaatcaccaa cattaaaacc taaaatgttc
     3421 atggcagttt taacaggaac tggatttact tctataaata aagcagcaat taaagcatcc
     3481 atatcaagtt gtaatttttt tgagccttca atatcacctt caaagaattt agttactaaa
     3541 tcatgtgttt ctttaggaca tatgttagca agtacagaga taacaccact tccacctaat
     3601 gataatagtg gtagtatagt atcatcatta ccggagtata tagcaaagtc tttaggtact
     3661 aatttagcta tttctgcaac ttgagctaag tctccacttg cttccttcac agcaactata
     3721 ttatctattt tagcaagttc agccacaaca gatggcttta tattcacttt agttcttcct
     3781 ggtacgttgt ataaaataat tggcaactta acgctatttg ctattgtttc aaagtgaagt
     3841 ttaagacctt tctcattagt cttattatag taaggagtta ttattaaaag accatccacc
     3901 cctaattttt cagcttcttg actcatatga acagacaaca tagtattgtt tgaaccagta
     3961 cctgctataa caggtattct cttattaact ttttcaactg tatattttat tacagcaaaa
     4021 atttcagatt cactcatagt agtcgcctca cctgttgttc cacaaacaac tagtgcatct
     4081 gtgccattct caatgtgata ttcgattaat tcacctaatt tttcaaagtt gacattatta
     4141 tcctctgtaa atggggtaac taaagcaaca gcagaacctt taaataacat ataatcaaat
     4201 ctcctttttt atctaaattt aattttttaa gtacaataat aattatagta caaattaatt
     4261 aattttaata ggtatagtta atatcttaac tgtttaaata agatatttat attatattaa
     4321 tattagaaaa attttaaata aatgttttat tctcaaaata tgcatattaa tttaataaaa
     4381 tataccaaaa tggaaataaa aaataaattt tatgagaatt taataactaa taattaatat
     4441 atttaggtta taataaatat gtgcgtaaaa tctaacaata agcaataata ccgtaaattt
     4501 aggaggaata atatggatac tttaaaggaa ctttttaaaa taggaagtgg accttcaagt
     4561 tctcatacta tgggaccaca aagagcagca gagagattta agaatgaaaa cccagatgca
     4621 gaaagtttta gagcaatatt atatggtagt ttggcagcca cagggaaagg tcatttaact
     4681 gactatataa ttgaaaaaac tatagcacct aaaaaagtcg aaattgtttg ggaagaagat
     4741 attataaaag attttcaccc aaatgggatg aagtttgaag ctttagataa agataaaaat
     4801 gtaacagctg aatggactgt atattctgta ggtggaggga ctatagcaga agaaggccaa
     4861 agaaacagta aaagcaatag tatatatcac ttagatacta tggatgagat agtaaaatgg
     4921 tgcaaagaaa ataataagac tttggtagat tttgtgttgg aatgtgaacc aaaagacata
     4981 aaagattata taaaaactat aaaagatgct atgagaaaat ctattgatga tggactaagt
     5041 acagatgaaa taataccagg caagttatta ttaaaaagaa gagcaagtac attttataat
     5101 gcatataaaa aagataagag cttctcaaca cttgtatatg catatgcatt agcagcttca
     5161 gagcaaaacg cttctggtaa tataatagtt acagcaccaa cttgtggttc tgctggagta
     5221 ataccaggaa tattctttgc aatgcaggat ttctataatt atgatgacga aaaaataatt
     5281 gaagcactac ttgtagctgg aataataggt aatataataa aaactaacgc ttcaatatct
     5341 ggagcagagg tagggtgtca aggtgaagtt ggagccgctt gttctatggc agcagcagca
     5401 gtagcatact taaaaggtgg aactatagac catattgaat atgcggcaga aatagcactt
     5461 gaacatcatt taggaatgac ttgtgaccct gtatatggat atgttcaaat tccatgtata
     5521 gaaagaaatg caatggcagc tcaaagagct tatgatgcag ctaattatgc attattaact
     5581 gatggctcac attctgtctc cttagaccaa gttgttgaaa caatgaaaga aactggtata
     5641 gatatgatgg ataagtacaa agaaacagct aagggtggac ttgctaaaca tttcttctct
     5701 tgctaaaaat aaaattttcc aataataatt cctctgtttt gatttatgaa acaggggaat
     5761 tattattaat ggtgaattat ttaataatgc aaaatatttt tatattcaat ttagcttttt
     5821 atttttaatt agtataacat gctatataat attttaatta taaagaaata tagaaatatt
     5881 ttttaataaa ttagaaattg gaggagatta catgagagag gcatcatatt tacaagaaca
     5941 actttataaa cataggaagt cattaaatcc tattgcagag attgggagaa aagagtataa
     6001 gacttcaaaa tatataagag attatcttga taaattagga attgagtatg aagtttattt
     6061 agaaacaggt atagtaggtg taatagaagg taaaagacct aaaaaagata ttgcttttag
     6121 agctgacata gatggtcttc atagtgaaga aggggtaaaa catttatgtg gtcatgatgg
     6181 acatgctact atattacttg gattaattga gtatttaaat gataataaag aagacttaaa
     6241 tgataatata gtatttatat tccaaccagc agaagaaggt cctgggggag caaaagcttt
     6301 aatagatgaa ggagtgctta gaaaatacaa tattgatgaa atatatgggc ttcatatata
     6361 tccagagtta ccagaaggtt atgtaggtac aagagagggc tattttatgg ctcaaatagg
     6421 agatattgac atagaaatag tgtctaaaag tggacatggg gctatgccac aaaatggtat
     6481 tgatggtata gtgatagctt caaactttgt aagctcactt caaactatag tgtctagaaa
     6541 tataagccca atagacaatg cagtgttgac tattggtaga attgaagggg gagctagaag
     6601 aaatataata gctgaaaata taaagatgga aggtactatg agatgtttca gcccagaagt
     6661 gtatgagaaa atgaagctta gagttagaga acttgctaga gggtttgaat tggcttataa
     6721 ctgtaaggtt aatataaata ttatagatga ttatattgct gttaaaaatg acaaggattt
     6781 gtatcaagag tttgtagagg ctattggaag tgatattgtt gtagagttag agccacttat
     6841 gatatcagaa gatttttctt attatcaaaa agaagttcca ggattatttt ttatgttggg
     6901 ttcaagaaac gaagaaaaag gatttgtaaa tggtcttcat aatataaact ttaattttga
     6961 tgaaaagata tgtgtaaatg cattgaatgt ttattcaaaa ttattaaaat acaaagaagc
     7021 aatagattag gaagataagt tataaagatt aaaaagcata aatacctaaa tttaaaaaaa
     7081 gtgtattaat aaatacccct tacatatttg tataaattta ttttaattga caaaaataca
     7141 aataagaagg ggtgattttt ttgaaagaaa aagtacaaag agaaaagaag ataactaatg
     7201 gcaagaatac taaaatattg acaaatgatg atataatgaa atatgagata gcctcagaac
     7261 taggtcttat ggataaagtt ggtgaacgtg gatgggcagg acttacagct aaggaagcag
     7321 gaagaattgg aggtatgctt acatcacgca aaaagaggct taaaaagatt gtggaggagc
     7381 agaataaaaa ggatgagtca atatagtgat tttgcgtttg tatatgatga acttatgaat
     7441 gaagttgatt ataatggttg ggttaaatat atagaagata taatagatag tgaaggtgta
     7501 aaagtaaaaa atatattgga attggcatgt ggcacgggta atcttacaat tccattgact
     7561 aaaaaaaatt acgatatagc tggtatagat atatctgatg agatgcttag tgtagcaaga
     7621 gaaaaggcag aaaaagaagg agtagaatta gttttacttc aacaagatat atcagagctt
     7681 gattttgaaa tatctgattt ggactgtgta ctttgtgctt gtgatggatt taattatata
     7741 acttatgatg atgatttgga aaatatattt tcaaagacat atgaattatt aaagaaagat
     7801 ggaatattca tatttgatat aagttctttt tataaattag ccaacatatt gggaaataac
     7861 atgtatggag aaaatagaga agatatagcg tatatgtggc aaaattactt tgatgaagaa
     7921 gagaatctaa ttgaaatgga acttacattc tttattaaag atgaatgtgg aaagtttgaa
     7981 agatttgaag agatacacca acaaagagca tatactgaag atgagatagt tgatatgctt
     8041 gaaaaatctg gatttaagga tataaaatat tatggggatt ttacatttga caaacctagt
     8101 gaagaaagtc aaagaatctt ctttgtatgt aaaaaataag atattcttga ctatataaga
     8161 taattattaa aaataaccat gatataaaaa tatagaaaat gcagttttat agattaaaag
     8221 taaagtgatg atactttatg acttttattt tttaataatt ataaagttct atagataaaa
     8281 attaaaatat agtaactatt acttagtaaa attaaaataa aatgggtttt gcccaaggtt
     8341 aagatagata agtatttata gataaattaa ggtagtaata taataaaaat aagacaatta
     8401 acgggacatg gaggaatatt aatgagagat tatgtactaa gagcgacatc aggaaatggt
     8461 caagttagag catttgtcgc aactactaga aatacagtag aagaagctag aagattacat
     8521 gaaactacaa aagtagcaac agcagcactt ggaagaacat taacagccac atctataatg
     8581 ggattaatga tgaaaaatga tagtgataaa ttgactgtaa taataaaagg tggaggtcca
     8641 attggaacta ttatcgccac atcagattct aagggaatgg taaaaggata tgtaggaaat
     8701 ccacaagtag aggttgaaga ctatccaaat ggaaagttaa atgtagcagc agcagtaggt
     8761 acagaaggtg ttgtaaaagt tataaaagat ttaggtctta gagaaccata taatgggact
     8821 tatccattgg taagtggaga aattgcagaa gactttactt attattttgc tgtatcagag
     8881 cagactccat cagtagtagc tttaggtgtt cttacaaaag aagatgaagt agagtttgct
     8941 ggaggattta tagttcaatt aatgccagat gcagaagaag agactattgc taagttagag
     9001 gaaaatgtag ctaaattacc ttctataact aacatgttaa aagaaggaaa atcaccagag
     9061 gatatattaa atattgtttt ggatggtttg gaacctaaaa tacttgatac ctgtgaagtt
     9121 gggtttatat gtgaatgttc aaaagaaaga gtaaaaactg ctttggttgc aattggtaaa
     9181 aaatcattgg ctcaaataat agaagaagat aaaaaagcag aagttggttg ccagttctgt
     9241 aataagaagt atatgtatag tgaagaagag ttgcttgaaa ttttaaagga aatgtagact
     9301 ataaaaatag ttgtattgat ataagtaatg ttatagtgat atagtaaagt cgtattgata
     9361 tagtaattaa agtataattt attaataata gacgtattta tattaaaaaa taagtttaat
     9421 aaatttttaa gcttaataaa ttttaagaat taagaagctt tctggctgta atgtttgcaa
     9481 tgttgcagtt agaaggcttt tttattttat tatagttcgt gtcaaagatt gtatgaaaca
     9541 ataaaatatt gtaaaataat ttgtatattt agacctgatt ggcacaaagg tttataaggt
     9601 atttgagttg attatgcgaa attctatgaa gtagtaaccc tgtgacttta atcatgggag
     9661 gttcagaaga aggagaaaat tttaaatatg gaaagtaaaa cgaagtacat aggaataagt
     9721 atattaatat tattgatgat atttttaatt gcaaagaatt ttaacggaaa acttgaaata
     9781 cctattcctg agagtaatag tagaaaattt aaatcagcag cagtgtttta tcctcaacat
     9841 caagatgatg aggttttatg gggaggaagt gccattgtag atgctataaa acagtgtggt
     9901 gttgataatg tatatgtggt attagtatca gatggttctg gagttaacgt tttcaaagct
     9961 aacacaaaat ttagaaattt aactaggaag caaaaggaag agcttagaaa taacgagttt
    10021 aaatcagctt tgagagagct tggagtgaaa cctcaaaatg tgattatact tgcagatata
    10081 gataaaaagg aaggcacaca ttatgaatta atggaaaaga ctatattgga ttttgaacat
    10141 aagtttaaag gcaatgtgac ccatatagcc catcactata agtatgatga ccatataatg
    10201 catagaaaga atggggaagt tttaaagaaa cttaaacgtg aaggtaagat aaaagatgac
    10261 ttgtattttg ttaagccaaa atatgtaaag tatataccaa gaaatgaaag agctatctat
    10321 aaagcaatgg attttgatga ctacaataag gttaagagtg cttgttatga atataaggtt
    10381 gtaaattcaa agcgtaaaat gtatggtata ggatatattt cggcacatag ttattttgaa
    10441 catcttctaa atgaccctga acttacgtct gtattaagtg aagattaatt tcttataagt
    10501 agtaagttat gtttgataag ttgattggaa ttttctatag ttttgaaggt attggaaatt
    10561 tagttttatt taaagattta aagatagagg tttttagtgt ttaaaatttt aaagttaagt
    10621 tgaataattg tatagaatta tttgtgaaag aagaaacagt gtaaaacata gtatcttatt
    10681 tatggtttta aaatcatagg caagatactt tttttatgta actaatacca ttttgagatt
    10741 attaacttaa gaattttata taatttaaca ctcatatata atgatgatat ttaggtagaa
    10801 tagatgcaat tacgcttaaa taaaataact gtatttttta taaaaatcaa accctacact
    10861 atactaccag ataaaatcta catgctataa tttgacctgt gtttataaaa aaaggagtaa
    10921 tctaataaaa tagtatttca cttatgtata actaacaact attattacga ttttctctaa
    10981 ataatttaaa gaaaaataat gcaggaggta aaatatgaac aatgaattta taattttaaa
    11041 aggttgcaaa gagaacaatt tgaacaatat ttccttaaaa ataccaaaaa gaaaaataac
    11101 catattcaca ggggtgtcgg gttctggaaa gtcatctatt gtatttgaga caattgcaaa
    11161 agaatcacaa cgccaactaa atgaaaggtt tagtacattt gtaagaagtt ttttacctaa
    11221 gtatggagag gttaaagctg attgtataga aaatttgtca acaccaatta tcatagacca
    11281 aagtagactt ggtggaaatt caaggtcaac cctaggtact attacagatg taaattcctt
    11341 tttaagagct ttatattcaa ggtttggtag tgagtatata ggtaatgcta atatgttttc
    11401 ttttaataat attgatggta tgtgcccaca ctgtcatggg cttggaaaaa agctagttcc
    11461 taatatgaac caaattcttg atatgaataa atcattaaat gaaggtgcta tattgctttc
    11521 agggtttgga gtgggttctt ggcattggaa tatattcgct gaatcaggat tttttgatat
    11581 taataagagg atatgtgatt attcagaagt agaattggaa aagtttttgc atggtaaagc
    11641 agaaaagatt aagatagaaa atgctggtca aactaacatg acatatgagg gattaatggt
    11701 taaatttaat cgcctatact taagtagaga gggagaaata tctgaagcta ctaagaaaaa
    11761 attgagtaaa cttcttatag aagatagatg tcctatatgt gatggaagaa gacttaatga
    11821 aagagtatat aggtctttaa ttaatggtta taatatagca gatttaacta gtatgcaaat
    11881 tgatgaattg gctgaagtta ttaaaagtat agatgagcca gaagcacaac ctttaattaa
    11941 aggaataata gaaaagttaa acagtatcat agatataggt cttggatatt taacactgga
    12001 tagagagaca tcttctctat caggagggga atctcagcgt ataaagatgg ttaagtattt
    12061 aaatagtaat cttgttgatt taatgtatat ttttgatgaa ccaagtgtag gacttcatcc
    12121 aaaagatgtg tataaattaa ataatctttt aaaaaaattg cgtgataaag ggaatacaat
    12181 tatagttgtt gaacatgacc cagatgttat taagattgca gaccatattg tagatgtagg
    12241 accaaaggca ggtaagtatg gtggagaagt tgtgtatgaa ggcagttatg aaaatctact
    12301 tacaagtggt actttgacag ggaatgcttt aagtaaattt cttcctatta aagaaagtgt
    12361 aagagaacat agtggatatt tagaagttaa aaattgtaat aaaaacaatt taaaaaatgt
    12421 atcgattaag atacctaaag gtgtattgac tctagttact ggagtagcag gagcaggtaa
    12481 aagtacgttg attaaggatg aatttttaaa gcaaaatcct agtgctgtac ttatagacca
    12541 aagccctgtt tcagcaaatt caaggtcgag tttggcaaca tatagtggaa ttatgaacaa
    12601 cataagaagc atattttcaa aaacaaatgg cgtaaatgct tctttattca gttcaaattc
    12661 agaaggagct tgtgataact gtaagggttc tggaatagta gaaatgaatc ttgcttttat
    12721 ggagagtata aagagtacct gcaatgtttg tgaaggtaaa aaatttaaaa aagaagtttt
    12781 agaatatagg tttcaaggga agaatataat tgaagttctt gaaatgtctg ttttagaggc
    12841 aattgagttt tttaatttaa aacagattaa aacgaaatta caatgtattg aaaaaatggg
    12901 tattggatac cttactttag gacaaactct tgatactttg tctggaggag agtgtcaaag
    12961 attaaagctt gctagtgaat taaataagga aagttcagtg tatatattag atgaaccaac
    13021 aacaggtctt catatggctg atattgagaa ttttataagt ataattgaag agattgtaga
    13081 taatggaaat acggtcataa taattgaaca caatatagat gtaattaaaa gagctgattg
    13141 gattattgag ctgggacctg aaggaggaac aaaaggcggg agagtaatat ttgaaggtat
    13201 tccaaaacaa ttatgtaaca ataaaatatc attaacagca aaatatatag tataataaat
    13261 aaaatataat aaaattataa tgatatttta ataaatataa tgggtatata aaatgataat
    13321 taaacttaac agtaataaat aatgtgagaa atattcaaat ttaattaaat aaagtgtcag
    13381 aaatttgttt aaattttagt attttaacgg ttctgacctt ttttcttatt tttatggaga
    13441 atatcgagga tggagtctct aatagttact tttttgtgtc tacttgcaag tttgattaaa
    13501 aaatggctat acacacttgt gacttcagtc atgggaggtt cagattataa tcttaatata
    13561 taatttgtga aataatttga tgacgatttg ctgatgtata aacaataaag attaactaca
    13621 ttaaaattta aatcttgtta agtaaaactt ataaaaggag agagatggat gataaatgat
    13681 attctaacct tattatctga aagaagtgat ttttttataa agctatccat agaacatttg
    13741 atgatttcta ttatttctat aataattgcg attgttttgg gtggtataat tggtattatt
    13801 gtaagtgaat ttcataattc ttctaaacct atattgggaa ctatcaattt tctatacacc
    13861 attccatcta tttctatgct aggatttctg ataccatttt ccggaattgg aaatactact
    13921 gctgttattg cacttaccat ttatgcatta ttgccaatgg ttagaaatac atatactgga
    13981 attactaatg tggataaaaa tattcttgaa gcagctaggg gaatgggaag tacagatttt
    14041 cagatattgt ataaaataaa acttccgtta gccctacctg ttattatgtc tggtattaga
    14101 aatatggtga ttatgacaat tgcattagct ggtatcgctt catttatagg tgctggaggt
    14161 ctaggtgtag ccatatatag aggaatcacg acaaataaca caataatgac aattactgga
    14221 agtatactta ttgctctatt agctttaata tttgatttta ttttagggct ggtagaaaaa
    14281 ttttttcaac gaaaaagaaa aaagaaaaat aggaaaaaag tttttgccat tctaggaagc
    14341 cttattctta tagcaactat ctttacgtct tatttattta atccttcaaa gactcaaact
    14401 attcatattg ctactaaacc tatgactgaa caatatattc ttggtgagat gttggatatt
    14461 ttaattgaaa aaaacacagg tctaaacgtg gaaattacac aaggtgtagg agggggaaca
    14521 tctaatatcc agccagctat ggagaatgga gaatttgaca tttatccaga gtatacaggt
    14581 acaggctgga atatggtact taagaaaaat gaattatata ctgaaagtat gtttagtaaa
    14641 ctacagtcgg aatacaaaac taaacttaaa atgcaatgga ctggtatgta tggtttttct
    14701 aatagctatg cattagcagt tagaaaagaa gttgcagata aatacaattt aaaaaatata
    14761 acggacttaa aagatgtatc ttctaaattg acatttggag ctgaatatga cttttatgag
    14821 agagaggatg gatatactcc attgtgtaat acatatggac tgaactttaa agatacagtt
    14881 gatttagata ttggattaaa ataccaagct atcaatgaag gtaagattga cgtaatgcct
    14941 atatttacaa ctgatggtca gtatagtgtt tcagatgttg tagttctaaa agatgataaa
    15001 aatttctacc catcttatca atgtggaaat gttgttcgtt tggaagtatt agaggaaaat
    15061 ccagaactat caaaggtatt taagatggtt ggagatattt tgactgataa agaaatggct
    15121 aaaatgaatt atgacgtaga aaacaacaat ttagaaccta gagaagttgc tcgtgagttt
    15181 ctgaataaga aaggtctttt ataaattgga ggaaatataa tgacacctat tatacaattt
    15241 aaaaatatta aaaaacaata taatgataaa actataatag ataatcttaa tttagatata
    15301 gaaaaaggag agtttttgac tgtaattggt tcgtctggtt ctggtaagac aacactctta
    15361 aaaatgatta atggtctgat tttgccagat ggaggaaata ttttgataaa ccaaacagat
    15421 atcaaaaacg aggacttaat taaattaaga agaagaatag gatattgtgt acagggaagt
    15481 gttctttttc ctcatatgac tgttgaagaa aatatttcat atgtaccaaa tctcctaagt
    15541 aaaaagaata aattagaagt aaaaagtgct gtaaacaagt ggatggaaat agtgggattg
    15601 ccaaatgata tgaaagtgcg ttacccatca gaattgtcag gtggtcagca acaacgtgta
    15661 gggattgcaa gagctttagc ttcttctcca gaaattttat taatggatga accttttggt
    15721 gcagttgatg agattacacg aaagcaatta caaaaagaga taaaagaaat tcataaaaaa
    15781 acaggagtta caattatctt tattacgcat gatatttatg aagctttgat tttgggaaca
    15841 aagactttgg tattaaatca tggagttgtt caacagtatg atactcctga gaatatttta
    15901 aatactcctg ctaaccaatt tgtagaccag ttattgaata taagaaaatc tataatagat
    15961 gatgataatt taaaagaata aatttaaatt taaaatcaaa ttgttgtgat aaaaactatt
    16021 gatttagagc taacttcaag ttgtatactt gaagtggaaa agttagatta aggtatagaa
    16081 gggcatttag cagttggctg tagacgaggt aactgctgaa ggtgctttca ataaatggct
    16141 agaacctgta tctgttgaag attataataa attgaaataa gaagctcaat atacagagta
    16201 gataggcact tcaaatagaa gtgtcttttg tgtgccataa aataaatcaa aatattagca
    16261 aagaatgtga aaaacaaaat aaaataatat gctatcttta aaataggagg gatgctatat
    16321 ggataacaat ttgattacca ttgaaaaaac tttagattat atagaaaaac atcttttaga
    16381 tgagatgtta gatttaaatg taatttctaa agagataggc tattcaaaat accatttaca
    16441 tagaatgttt acatcagttg tcggatttag tgtacataat tatatacaaa gacgtagact
    16501 tactgaagca gcgagaatgc taatttttac agaaaaacca attattgaga ttgctttaat
    16561 ctcggggtat gatacacaac gctctttttc aagagggttt aaaaaaatgt ttaaacgtag
    16621 tcctaatttt tttagaagac atcaagattt tttaccacta caattaaaat ttgatattca
    16681 taatagaaaa aaattacgtg gagatatggt tttagatgtt aaaaaagtta attgtgcaga
    16741 tataaatatt gttggctatt ctggtaaagt aagaaaagga tttcatatta ttggaaaatg
    16801 ctggagggat ttgcataaaa ataaagagat gatagaaaat aaggtagatt taggtttttt
    16861 aattggagtt agtgattata gcaattttaa agagattgag aatactccta catttacata
    16921 cattgcaggt gctcaagtag ctacttttgg caaattacct aatggaatgg aaaaattcgt
    16981 attaccgaaa agtaattata ttgtttttac ttttattggg aaaaatgagg atagtatgca
    17041 aaatgttgta gaatatatct atcaagaatg gttccctaat tcaagttgcc agtttaatga
    17101 aaataattta tatgactttg taaaatatgg agaaaaaact gatgagaatg gtttgagtga
    17161 aattcagttt tgggttccaa tcatttaaaa gaaggtatta tgtataaagt agatggattt
    17221 atataggcaa agttttacag cagtatgtat ttataaataa tttatagttt gtaggaaaga
    17281 aataatttta ctataagatt atgataaaat acacaatact atgacaaaat agtgttaaat
    17341 aatagattta attttgcata ggaaaagttc tataatggtg tgtattcata aataatttat
    17401 aatctgtagg aaagaaatag ttttactata agattatgat aaaatacaca atactatgat
    17461 aaaatagtac taaataatag atttaatttt gcaaaggata aagtgtttta ataaaaatga
    17521 atgtagaaaa cgaattactt aaaaacctag atagattgca tacaacagag ttaggcgttg
    17581 taagaattaa gaaaaatctt tctttggaga caaatgatgt agtgaactgg tgtaaaacta
    17641 aaatagaatc tccaaatgcc attattaata gaaagggaaa aaactggtat ataagtgtat
    17701 atgattgtat tataacagta aacgctcaca gttatacaat tattacagct cataaagaaa
    17761 agaaatgaat tgcaaaatac aaaggtcaaa agcattttcc actgtttgta gtacctatga
    17821 agaagtatat aaaataatat tacttttgaa taataattta aattttggag gttgaaaaaa
    17881 tgaatgatac tgtaaaagaa aaattggcag agtcactaac ttctgatact actgatattt
    17941 taccttattt gccttattta ttacaagatt tttgggaatt aggaagttca ccagaacaaa
    18001 tgttacaatt atttagaaag catatacctt ttaatgagga tacaaaagtt ttggatttag
    18061 cttgtggaaa aggtgctgta tctgtgaagc ttgcaaaaga gttgaatata catatcaaag
    18121 gtattgattt gatacctgaa tttattgatt atgctagaag aaaggctatt gaatataagg
    18181 ttgatgcttt gtgtgatttt gaaatgaatg atgctaatag ttctgtaaaa attgagcatg
    18241 attatgattg tgtgattttt ggagctgtag gggatatttt aggtaattat aaagaaacta
    18301 taagtaagct attacctaca ataaaaagta aggggttcat tttaattgat gatgcatatg
    18361 tagtagataa atctacaaat gaacagctta attttgagag ggtgtatccc acttacaatg
    18421 agtggtgtat attgttcaaa gaattaggtc tgaagctaat tgaatgtata ccatgtttag
    18481 aacttgaaaa tgatgttgaa gaggaaatga attggattat aactcgtgca aatgagctaa
    18541 ttaaaaaata tccttctcaa aaagatatgt ttgaacaata tgttattagc caaaaaaatg
    18601 aatatactga tttacagcag gaccttgttg gagcaacttg gattttacaa aaagaataag
    18661 ataaatatta gactagataa tcaatgttat agtgaattat cccatgtcta gtgaatataa
    18721 ataacataaa aaaccgtcaa gatgaatagt ttattcgtgc ttgacggttt ttagtatttg
    18781 ttgaagtaaa attttatttc tttagtatat cacttttagt caatccattt gatttagctt
    18841 gagtattcca ttgatgattc cccataagtt tatataggaa tctagggaag ttaggtgaaa
    18901 taaaaatagt acttttgttg gaatcatcta tatgtatatt atcactaagt atattttttg
    18961 aaagttcttt taatgcaatt cctaaatctt ttttaggtcc tttacccaaa ggtacggatt
    19021 ctagtgaacc aaacatctcc ccagctccaa ttccaatacc ttgaacccat gtaaagttga
    19081 tttttttaca ccaatgctct atcatttgaa ttgccaaatg attatgttca ccttcattga
    19141 atccacagtt aactattgca tacactggaa taggtttttt attttgtgtt tttaaaaatt
    19201 tttcacattt ctctaaaaaa tgaagtaaat gagatggaat accatctaca taaagaggag
    19261 atgcgattat taatctatca caattaagta tggcattaag agcgtcagga tacttagatg
    19321 tgttatctac tttaaatgta aaaactttat tatcattttg aataagttgt tttaattcag
    19381 ataaaaaata ctgtgaatta tttttattta cttttggact accatttatc attactatat
    19441 tcatatggat tcacctctta tatcttgtat attttcatag aaacaaacag agtgttcttt
    19501 tatattgaaa tttatagcat ttccatttac taatttcgta gcagtttctt tttcagcttc
    19561 tgtaatacaa tctccataga atatagcttt taaatttatc tgtttatcat atctgctttt
    19621 atgacgcatc atattatctt ttattacaaa gtatggaaga atataattaa tgcttttatc
    19681 aagtacgttt ttgacaaaag ggctaaaccc tccgtaacag catctgctta ttataataat
    19741 tccatcagag tcatttataa gtttacccat gttgctgtaa taatctttta caacacattc
    19801 agaaggagtt ttaatccagc aaccaaaaca accaatacat ttgtggattt cttttgtgtt
    19861 tggaagtatt tttatattgt catcaagttt gggtaataac ttttctaatt ctttgtcagt
    19921 taaatcatgt attaaaagcc tcataaagtc catctccttt tattatgtat gtttacttct
    19981 aaaaattata tcttatagat ttttcagtaa atctttacac caattaataa caaaattata
    20041 atagtcaact ccataaatca ttgttgcata catatatgta tattggtcaa cacaatgatt
    20101 ttcttctgca tcattttttt gtttacttaa tttatttaga agtaattcta cttcagttat
    20161 taacattttt aagtttgttt caattatttc tttgggtaga tacttgtaaa agaatatatt
    20221 aacaaggtgg tcttgctttg ttttagaaaa atttataggt ttctttaacc attcaagaaa
    20281 tacttcttta ccagtattag ttatggaata tagttttttt aatttgttac catctataac
    20341 ttcgtgatag tgtatatatc cttttgtttc taatctttta agtgcaggat agatacttcc
    20401 aaagctggcg tcaaaaaagt acgaggtact ttctgacatt atttgtttta agtcgtatcc
    20461 acttaattct ttttccatta aaaatcctaa aataatgtat tctaacatat tacctcctgg
    20521 tatcaattag atatatatcg attcgatata tttattatta cagataataa atcttacgtc
    20581 aagatgcttt ttaagataag ctaaaaagaa ttaggagtta ttgataaata tgcaaaaaaa
    20641 ttaatataaa aggtaattaa atatcaataa agatttaaaa atagttaaat caaagtaaga
    20701 gtctaatata taaaatttat agtaacttta tggtttaaat ataaacttgt gtgctattga
    20761 caaagtataa aattagcagt agaataattg catgagcaaa tagattgttg cgaacgcaaa
    20821 aaaaggacaa tagatattaa aggagagaat atgagagagc tattaaaatg ggtttccatt
    20881 ataaatagat acaatacaaa ttatattgac aggaaattgg cggatattgg aatcaataat
    20941 agccaatatt tttatgtatt acacatatgt aataatccag gtattacaca ggacactata
    21001 tttgagaaaa ttcttgtgaa tcctagtaat ataacaagag ctttggcaaa tttagaaaaa
    21061 gaaggcttta taacaagaga accaggtgta aaggataaaa gaacatggca tttatatcct
    21121 acagaaaaat ctttaggatg ttatgatgaa atactaaaaa taacaaatgg gtatgttgag
    21181 gagttgttga gttcatttga gaaagaagag caagagttgt ttatgtcaat gttaaagaaa
    21241 gttgcatttt tggctattga tatgaatgaa gaagcaaaat tggaggcgaa ggaaaatgga
    21301 agaaaaatat aaggagccat tggaatatga aaaggtttca aagttattaa ctaagtacgc
    21361 aattcctagt attatagcaa tgttagtaag tgctttatat aacattgttg accaaatatt
    21421 tattgggcaa ggtgttggtg tattgggcaa tgctgctaca aatgtcactt ttccactaac
    21481 aacaatatgt actgcaattg cattattgtt aggaattgga ggagcgtcta agtgttcatt
    21541 ggaattaggt gctaaaaatt cagaaaaagc tacaaaagca gcaggaaatt ctatatgctt
    21601 gatggctgtt tttggaataa gtttatttat agtagtatca atatttttaa caccactatt
    21661 aaaattcttt ggctctacat cagaaatact accttatgca cacacatata caaaaattac
    21721 atcaattgga ttaccatttc ttataatgtc cactggaatg agtaagttga tacttgcaga
    21781 tgggagaccg aaagcttcta tgttttgtat gttaatagga gcagttatta atacaatttt
    21841 gaatccatta ttcatttttg tttttaatat gggaattgct gggtctgctt tagcaactgt
    21901 tattgggcaa cttatatctt ttggaataag tgttcattat ataaagaatt ttcaacatat
    21961 taaattacat aagaaaagtt ttaaattaga tagtataatt tataaacata tatttgcgct
    22021 aggttcaagc tcatgtttta atcaattagc aatgactgta acacagatag ttatgaataa
    22081 tacattggca tattatgggg ctaggtcagt ttatggaagt gagattccac ttgcttgtgt
    22141 cggtatcatt attaaagtaa atatgatttt tatatctata attattggta tatcacaagg
    22201 tatgcaacca attatagggt ataattatgg ggctgaaaag tataatcgtg taaaagaagc
    22261 atataagttt gcagtgggaa gtgcaactat tatatctgtt attgcattct tatgtttcca
    22321 attattccca agacaaatta cgtctatatt tggtaacgga aatgagactt attttatatt
    22381 tgcagaacat tattttagaa ctttcttgtt cttaacattt ataaatggtg ttcaaatagt
    22441 atcatcaact ttctttacat caattgggaa atcagtgttg ggacttttta cttctttgac
    22501 tcgtcaaatt ttatttttaa ttccattgat agtaatttta ccaatgttta tgggaattga
    22561 tggagtaatg tttgcaggac caatagcaga tgcagcagcg gcaatggtat gtatatactt
    22621 tgcagttcat gaaatgaaat cattgactat aaagcaaaat aatttgatga gtaaccataa
    22681 taagattgca gaggtataag acaatattta agaagtatag aaaattaatt taaagaagta
    22741 taataaacta atttaggaag tatagaaaat taatctaaag aagtataata aactaattta
    22801 gaaagtatag aaaattaatc taaagaagtg taataaacta atttaggaag tatagaaaat
    22861 taatctaaag aagtgtaata aactaattta ggaagtatag aaaattaatt taaagaagta
    22921 taataaacta attt
//
LOCUS       DAGSRM010000059        22819 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.059, whole genome shotgun sequence.
ACCESSION   DAGSRM010000059 DAGSRM010000000
VERSION     DAGSRM010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22819
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.059"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            589..1518
                     /gene="glsA"
                     /locus_tag="MJE33_002406"
     CDS             589..1518
                     /gene="glsA"
                     /locus_tag="MJE33_002406"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBY9648338.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
     gene            1616..2179
                     /locus_tag="MJE33_002407"
     CDS             1616..2179
                     /locus_tag="MJE33_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY9648339.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            2667..3503
                     /locus_tag="MJE33_002408"
     CDS             2667..3503
                     /locus_tag="MJE33_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBY9648340.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     DEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHVGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKRELKMIKEILS
                     SK"
     gene            3787..4782
                     /locus_tag="MJE33_002409"
     CDS             3787..4782
                     /locus_tag="MJE33_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBY9648341.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRDKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADEVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKEYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(5039..5443)
                     /locus_tag="MJE33_002410"
     CDS             complement(5039..5443)
                     /locus_tag="MJE33_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBY9648342.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     LLFDFIDKDNPRVEIELYPVSKYDISYNIFEK"
     gene            6540..7454
                     /gene="queG"
                     /locus_tag="MJE33_002411"
     CDS             6540..7454
                     /gene="queG"
                     /locus_tag="MJE33_002411"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBY9648343.1"
                     /translation="MDNKELKAFCNLIGLNCVGVAGVDKYDNLEKILKDRQQKGYLTG
                     MEEPIIENRINPRHIMKDANSIIVCAFPYHINRQEKNKNSNLSKYCHGKDYHIVVKDF
                     LQQICDYISKTVTDFKYKLFADNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYVFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSEK
                     EKVVLKNGKKVFGCDICQEVCPHNVEIPTTHILEFKENIIDYLDYDEIQNISNKEFKR
                     RYGDRAFSWRGRNIIKRNMEIILDKYDD"
     gene            7560..9797
                     /locus_tag="MJE33_002412"
     CDS             7560..9797
                     /locus_tag="MJE33_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBY9648344.1"
                     /translation="MKYNNTSEINPLDYILGQERAVEAMELGLKINNPAYNIYIAGEP
                     GTGKSTYALKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNKLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDESDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKIQTYLENIKKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNGSSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEDNEEGIARFIAYQCDKNNLRHFTKSAVEEVIKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTEQCDVKKAISEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVSIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVRDGKFKIYTVERVEEAIEILTDVKFEEIKKLVKEKLISFSKIQ
                     TVSKE"
     gene            9994..11046
                     /gene="nth"
                     /locus_tag="MJE33_002413"
     CDS             9994..11046
                     /gene="nth"
                     /locus_tag="MJE33_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBY9648345.1"
                     /translation="MKNVNLKEINEKDVIVEEDINLKGMDKKDVIEKEEVNKKSIDKK
                     DIIEKEEIDKKNVKKVEVQKGIDKMIGKKDEIIDEKDIENFMDIEEDKKSIKVSKKST
                     KKGETEKKTKKSSSNKATQKTSKETKSKSKIKNESANEKDVNKILDELEKLYPDAKCE
                     LNYGTAFELLIATILSAQCTDVRVNKVTSELFKKYNTARDFANLSIEEISKEIKSCGL
                     YKSKSQKIKDTSEQLCELYDGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDT
                     HVFRVSNRIGIVDEPNPQKTEFALMETIPKERWSHSHHVLIFHGRRMCKARNPECASC
                     PIKEDCNYYKELNETK"
     gene            11240..11710
                     /gene="trmL"
                     /locus_tag="MJE33_002414"
     CDS             11240..11710
                     /gene="trmL"
                     /locus_tag="MJE33_002414"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBY9648346.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            11746..12594
                     /locus_tag="MJE33_002415"
     CDS             11746..12594
                     /locus_tag="MJE33_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBY9648347.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEREYKDRIDI
                     SGDEFYKRLREENVYPKTSQATYAQFKEIFEKYTNEDREILYIAGSATATGTYQSAVM
                     AKNDVDGKIYTYDSNLLCMGTGLLVIKAGELASEGKSVAEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMMELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMQDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
     gene            12950..13954
                     /locus_tag="MJE33_002416"
     CDS             12950..13954
                     /locus_tag="MJE33_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBY9648348.1"
                     /translation="MRFKRILILMLTVAMIAGMLVGCANNPSNNPSSSKDNKSDISDK
                     SNSKKSVSMILDIEGTNNEAMNNSALLALNNAQKKLNIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFIGGTKSSSRDKFESGFREGVKFSNSSIRDISVEYADVFKDSK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIDAVRAKNKKVIATNKDQHELAPENVISSV
                     VKDFENPTYNLIQSFVKGNYKGGKVIENTVKSGSTGLAQNSSQNIPPDVLEYVNKNNK
                     "
     gene            14080..15192
                     /locus_tag="MJE33_002417"
     CDS             14080..15192
                     /locus_tag="MJE33_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBY9648349.1"
                     /translation="MKIKRILMACFVVVFTIFILAICNFDFGFDKVNKNNSNIDSAKS
                     KQNESKSLPTSAEKDKSNSKSKNKEDDTEDDSYVRKTIGVLLDTENLNNESYNNNVLS
                     ALKRADEDFDVRIKLEKPKTSKSFKESIEKLHKYEPDLIIAVGARFAEPIKEAATKYP
                     KQQFAIVDYTYKVQPANVISVSFQDNVTGYLAGLIAGKMTDTGKVGFIGGVEGSSRDK
                     YESGFRKGLKTSNKDAELLVKYSNIFENPESARTIAKEMRDSDRDVVFSATEDDSKRA
                     IQVARENGGKVIAINKDQYDMAPDNVISSIIKDVEQPVYELIKNVAKDGFKGSKVMEF
                     KIKDGELGLTSKSSRNIPPDVLEYIKKEYNKVKDTY"
     gene            15548..16309
                     /locus_tag="MJE33_002418"
     CDS             15548..16309
                     /locus_tag="MJE33_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648350.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTIEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            16312..17130
                     /locus_tag="MJE33_002419"
     CDS             16312..17130
                     /locus_tag="MJE33_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HBY9648351.1"
                     /translation="MVEKDNISSMKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKK
                     ILNGTKTEQRALNIDEISTEITSMIISIMKEKLLKEYILKCYGRTYPEDKNNIYIYSL
                     NIFAKKEIFMRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLI
                     KKDQDEFIRVLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKNGNKIDSIDDEEIINMVI
                     RENLNYEDFLISTLLTLCPKKIEILDLLNNNCSKEIIDTVEAIFENKVSIIMEN"
     gene            17310..17912
                     /locus_tag="MJE33_002420"
     CDS             17310..17912
                     /locus_tag="MJE33_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HBY9648352.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIEPIKIPILNIE
                     IVGLIPKRRAEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPSGIKNMIQDYLGEIIESEVKQSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIAQGLITMFI"
     misc_binding    18371..18608
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            18672..20591
                     /gene="thrS"
                     /locus_tag="MJE33_002421"
     CDS             18672..20591
                     /gene="thrS"
                     /locus_tag="MJE33_002421"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBY9648353.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSDEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
     gene            21359..22450
                     /locus_tag="MJE33_002422"
     CDS             21359..22450
                     /locus_tag="MJE33_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBY9648354.1"
                     /translation="MIEKVEEKSKIIKTSKEYKRTIIEEKDKEWTKKLNDILSRSSFS
                     NDELDKIKALIPKEILTNENVGEVVTEDGYAKPEYDEVFKSLFTLNNEYDLLVNFIND
                     ILKDAPYANRNIKPFTQIKRIIRVETDPTINYIGEKRPRLDILAEDEENNHINIEMQR
                     AFENDYLERAEYYLSRVHGRKLEEGKEYKEIGKTIGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIYSSKTALYFIELPKIHKSDYIVNRIMLWGKLISNPSSLDVRVIAKNDSIIK
                     KALDRLKELGSNEEYLLNLKRGAYIMNKSRNFKEEIERETAIRVAKEKDYKIIIMLKK
                     NGFKLDDILNKFNDFDLSEDEIKEVYENN"
BASE COUNT         9310 a         2105 c         4176 g         7228 t
ORIGIN      
        1 gaaatttttg gtgttataaa aatttaaatt actggattat tagattgctg gattgctaaa
       61 ttactaggtt gttaaattat tgaattatta ggttgttgaa ttactaaatt actaggttgt
      121 taagttactg gattatttag atagttagat tactaggttg ttaaattatt gaattattag
      181 gttgttgaat tactaaatta ctaggttgtt aagttactgg attatttaga tagttagatt
      241 actaggttgt taaattattg aattattaga ttgttgaatt actaaattac taggttgtta
      301 agttactgga ttattagata gttagattac taggttgtta aattattgaa ttattagatt
      361 gctaaattat taggttgtta aattactgga ttattagatt actaaattgc taggttgtta
      421 aattattgaa ttactaaatt attaagctct ttaaactatt taaattgttt gaaaaatgaa
      481 ttgattcttg tagaaaataa ttttaaagta taattttttt taacattgtt ttattatagt
      541 ataaacttgt gctaaaatag aatatataac ataatataag aggtgcatat gaatacgtta
      601 gatgagacat tattaaaaga aattataagt tctaataaga agtatactaa ttatggacaa
      661 gtcgctagtt acataccaga attaaaaaac gctagaagaa atgatttagg aatttgtatc
      721 atagatagcg aaaataatct ttatagtgct ggaaactgta gcacaaagtt tactatacaa
      781 agtatttcaa aaccaattgt gcttgctatg gcacttatgg ataatgattg ggaagacgtt
      841 ttttcaaacg ttggaatgga gccaagtggg gacccattta attcaataat gaaacttgaa
      901 attaatgaca caaaaaaacc ctgtaatcca atgataaatg caggggctat agtaaccact
      961 tcccttataa atgggagttg tttagaagaa aaagaagaaa gaatgcttag tttctttaga
     1021 aagttagcaa aaaatgataa tataggaata aactatgatg tctataagtc tgaaaaaatg
     1081 actggagata gaaacagagc tatggcttac ttattgaaaa gtgatggatt tatcagaggg
     1141 aatgtagaag atgttctaga cctttatttt aaacaatgct caattgagat agattctgta
     1201 gatttagcga gaattggaat aaatcttgca aattatggag tagatataga aaatggtgag
     1261 catttaatga gtgaaatggt ttctaggatt gtcaagacat ttatgatgac ttgtggaatg
     1321 tatgatgctt ctggggaatt tgctattaaa gttggcattc ctgcaaaatc tggagttggg
     1381 ggtggtataa tggcttctgt gccgggacgc atggggattg gtgtttatgg acctgcgctt
     1441 gacaaaaaag gtaacagcgt cgcaggagtt aaagttttag aggagttgtc aaataaactc
     1501 aaattaaata ttttttaaaa tattaagaaa ggattttttt gacaacaaca tataatttaa
     1561 tatataatta aattgtaaag aaatatgaat taaataaatt ttttaggagg ggtcaatgaa
     1621 agaggtattg gtattacgag atttggagtg cataaaggct attgcgcatc cacgtagaat
     1681 agatattcta aaagcatttg acaaatcgcc attgtcagca aaacaattat ctcaattatt
     1741 ggaagaacct catgcaaaaa taaactacca tataaaaatg ttatacaaag ttggtatttt
     1801 agaattagtt gaggaaaaaa taaagtcggg gattgtggaa aagtactatt atccaagtgc
     1861 aaaaaatgtg gttataggga ataggatact taatttttct ttagacaatg gggaagaaaa
     1921 agaagaattg tacatatcaa aatttgaaaa tatgagtgaa gttttttata aggcagcaga
     1981 ggaagatgtt ttagaaaatg aaaatatagt tgattatcat gatatttctc ttactcatga
     2041 tgaattagtt gaacttagtg acactatgaa gtcaaagata gatgaaattt taaataaaag
     2101 acaacacaat gttgaaggtt ctaagtatga tatagctatg gttatagttc ctactcttga
     2161 ggaggaatgt cctagttaga aaattaatta tggattaatt aaagatataa tattagagat
     2221 tgaacatcaa aatttactta ataatcaaaa aatagttttt ggtatgggta aaacaatgat
     2281 ttttaatctc tatttttttg ttttcttata cctattgtat ttgtaaataa tgtgtatagt
     2341 gtaagatgta tacactatct aagaagtgct tagataatat acaaagtatg taagattata
     2401 atatttgaaa ttgattaaaa tgggtataat taaagtgtaa atattaaaat taaaaattaa
     2461 tataaattaa atcaatgcta gaaatatgtt aaaagaggaa tgagattagt tgggatagaa
     2521 catcccttag agcttatgaa tttttagtga aaaagtacta tcaaatagga atctccatct
     2581 tcatatgttg gctaaatgta gttaaggtgg agtagttcaa aattaaaatt gagtacaagt
     2641 cgtagtacaa ataaaaggag attaatatga ttaaattagg aacacatctt tcaatagcaa
     2701 aaggatttgc taatgcggct gagacagctg tatatattgg tgcaaataca tttcaatttt
     2761 ttagtagaaa tccaagagga ggaaatgcta aagaatttga tgaaaaagat ataattaaat
     2821 ttcaagaaat tagaaaagaa aataattttg caccactgct tgcacatgca ccatatacta
     2881 tgaatctggg tggtacaaag gatgatgtat acgaatttgc aacaagggtg atagatgagg
     2941 atataagaag gatggactca ttaggaattg aatacatgtg ttttcatcct ggaagtcatg
     3001 taggtggtgg tgtagatttt ggtatagata gaatagtaaa aggattgaac aatgccataa
     3061 agggtgatga aaatataact atacttcttg aaactatgtc gggaaaggga actgaaatag
     3121 gttttaaatt tgagcaatta aaaagtataa tagacaaaat tgagcataaa gaaaaaatag
     3181 gtgtgtgtct tgatacatgt catatttttt cagctggtta tgatatagtg aatgatttag
     3241 atggggtttt ggaagaattt gataaggtta taggcataga aagattgaaa actgtccatc
     3301 taaatgatag tatgatgcca tttggagaca aaaaagacag acatgctcct ataggagaag
     3361 gaaagatagg attaaaagca ttaattgatt ttatggaaca tcctagtcta aaacatttac
     3421 catttttctt agaaactcct tttgatgatg aaggtcacaa gagagagctt aaaatgataa
     3481 aagaaatact atctagtaaa taaaccttta ttaaaaatat gtgaaatact ccgccctcaa
     3541 cataattagg gtggagatat aatcatgaaa tgtgataatt taaattaatg gataggaata
     3601 acaaaaattg tatacaaatt aactgggtat aaagtatgat tatattatga atagactgat
     3661 ataaaatata attatattat gaatagtatg taaatatatt gcaaagattc tcataatttg
     3721 ttgtataatt aaagatgaca tatttatatt atataaaaaa ttattaaaag ttaggtgggg
     3781 aaagatatga gaaatttagg caatactaac atgaaaataa agagagttgg ctttgggggc
     3841 attccaatac aaaggattac tcaagatgat actaacttag ttataaatga gttagaaaaa
     3901 caaggaataa actttattga cagtgcaaga gggtatacaa taagtgagga agcaatcgga
     3961 attgccattg aaggaaaaag agataaattt ttcttagcta ctaaaagtat gtccagagat
     4021 tatgattcaa tgaagagaga tgttgaaata agtttaaata attttaaaac tgattttata
     4081 gatttatatc aattccataa tgttaaagaa gaggagtatg ataatctttt taaagataaa
     4141 atggcttata gtgccctttt agaggcaaag gagcaaggta agataaaaca tattggtata
     4201 actagtcaca atttaaatac tatagaaaaa gctatagaag atggaaaatt tgacactata
     4261 caattccctt acaatatagt tgaaggacaa gcagatgaag tgtttaaaaa agctcatgaa
     4321 aaaggaattg ggataatagt aatgaaacct ttagctggag gtgctttaga caatgcaaca
     4381 ctggctatta aatatatact atctaaagag tacatagatg tagttatacc aggtatggag
     4441 agtgttgaac aggttagaca aaatgtcgct gtacttgaaa atcttgtgct agatgaaaaa
     4501 gataataagg aaattgaaga aataagaaat tctttaggta aaaaattctg tagaagatgc
     4561 gaatattgta tgccttgtgc tgtaggaata aatataccat tgagtttttt atgtgaaggt
     4621 tattatacta gatatggatt aaaagagtgg gctaaagaaa aatatgaggt tatggatgtt
     4681 aaacctactg agtgtattga ctgtgggctt tgcgagagta gatgtccata tgaacttcct
     4741 ataagagaaa tgcttaagac tgttgttgaa aagctaggat agattttatt tattgaaatc
     4801 ggtgtattaa atttactccg atttcttttt attagatgtg gtcttatata caaatattaa
     4861 atttacatag ttatagctat tatataaatt taatatttgt ttaatactta ttaaatattt
     4921 acttaatgct ttttaaaaac atttgtttga gtatagacat aacacaatta ttattgttgt
     4981 attagtatag tttcaaatac tggagtatca atataatgtc gaataacaca taaaatcttt
     5041 atttttcaaa aatattatag gatatatcgt atttagatac tggataaagt tcaatttcaa
     5101 ccctagggtt atctttatct ataaaatcaa ataaaagtac tggcttaagt tgtttatcat
     5161 tttttattat tccacttttt tcaataccat cacaaatact ttttggataa ttatgtaaat
     5221 cccccattct cttatttggg aagaataatt tcaagactgt tattagattt tcttcaacag
     5281 tttccccttg atactgttgg tttataaaac ctgcaatcat gttttcgtat gcaagatact
     5341 tagaatattt tccctttgaa ggcatccatg cttgaccatt tatgttatga agcttaaaat
     5401 tagatttact aataggtcta cctggaattg ttattttaat caaatttttc accatcctta
     5461 tacagtattt attataatct gtatatgatt taaaagcaaa ttgttattta aagtgtttta
     5521 cagagctttt ttgtaaaatt ttccacttta gatgtagtaa tataagataa gtattttgct
     5581 tacaaatttt aaaatttgat atatgatgga catattcata ttagactatt ataagacctt
     5641 tataatatta ataagtatag tttagtaaag agagtaaaaa gaaatccata taagtcaagt
     5701 aaagacttaa taaatggcaa tgtaaatggg atattaatta taagaaaaaa tcaatctttt
     5761 tgcttaacta tagaaaaaat gtcaaaatat aactaatgat atttgaagtt ttagagttta
     5821 tattaaaagg taagaaggtt aagtgggtaa aaaaggttga atagattaaa aaggctaagt
     5881 atctaaaaga gttaagtagg taaaaaagtt aaataagtaa atgaaaaagg ctaagtatct
     5941 aaaagagtta gtaggtaaaa aagttaaata agtaaatgaa aaaggctaag tatataaagg
     6001 agttagtaga taaaaaaatt aagtatctaa aagcgttagt agataaaaaa attaaataaa
     6061 taaaaaagac taagtattca aaagagttag taggtaaaat aattaaatag ataaaaagac
     6121 taattattta aaggagttaa gtagataaaa aagttaaata agtaaatgaa aaagacgaag
     6181 tatctaaaag agttagtaaa taaaaaagat gaagtatcta aaagagttag taaataaaaa
     6241 agatgaagta tctaaaagag ttagtaggta aaaaactaaa taggtaaaaa gatgaagtat
     6301 ctaaaagagt tagtaggtaa aaaattaaat aggtaaaaag acgaagtacc taaaagagtt
     6361 agtaggtaaa aaaattaaat agataaaaag acgaagtatc taaaagagtt aagcaggtaa
     6421 gaaaattgaa tagctaaaaa gattaagtat ttaaaagagt tagtagctag aaaaggttaa
     6481 aaagactaaa aaaattaaat aaatttaaaa agtcaattag attaaaaagg aataatttta
     6541 tggataataa ggaattaaaa gcattttgta atttaatagg attaaactgt gtaggagtag
     6601 caggggtaga taagtatgat aatttagaaa agatacttaa ggacagacaa caaaaaggtt
     6661 atttaactgg tatggaagaa ccaataattg agaacagaat taacccaaga catataatga
     6721 aggatgctaa ctctataata gtctgtgcat ttccatatca tataaataga caagaaaaga
     6781 ataaaaattc aaatttatca aaatattgtc atggaaagga ttatcacatt gtagtaaagg
     6841 actttttaca acaaatttgt gattatattt ctaaaacagt aacagatttt aaatacaagt
     6901 tatttgctga taatggacca ttagttgaca ggtatttagc ttatttatca ggaataggat
     6961 attttggaat aaataataat atcataactg atgaatatgg ttcttacgta tttatagggt
     7021 atatagtaaa taattataaa tttgaagaag ataaaccact agaaaaaagt tgttttaaat
     7081 gtggtaagtg tgtaaaatat tgtccgggaa atgctatatt gggaaattat gatatgaacc
     7141 ccaaaaaatg tttatcatat ataactcaaa aaaaaggaga tttgtcagag aaagaaaaag
     7201 ttgttttaaa gaatggcaaa aaggtatttg gatgcgatat ttgtcaagaa gtatgtccac
     7261 ataatgttga aataccaaca actcatattt tggaatttaa agaaaatata atagactatt
     7321 tggattatga tgagatacaa aatatttcaa ataaagaatt taaaaggaga tatggagata
     7381 gagcttttag ctggagaggg agaaatataa taaagagaaa tatggaaata attttagata
     7441 agtatgatga ctaataataa gagtctgttt catatacttt aatgtgtatt tactttaaca
     7501 cttgttgtgt aaaagtcata ttatatcatt aaagttaaat atggggaggt gggggaatat
     7561 tgaaatataa taatacatct gaaataaatc cactagatta tatattgggt caagaaagag
     7621 ctgtagaagc tatggaatta gggttgaaaa taaataatcc agcgtacaac atatatatag
     7681 ctggtgaacc tggaactggt aaaagtactt atgccttaaa ggttttaaat gagtatgcat
     7741 ccaaaaaaaa tacgcataaa gattggtgtt atatatataa ttttgaaaac cctagagagc
     7801 ctattatagt tgaattagaa aaggggtttg gaagagaact taaaaaagat atggagaaat
     7861 taattgaaag tcttttagaa gattttaaaa atgcgtttga aagtgaaaat tttgaaatag
     7921 aaaaaaataa acttctggat gaatatgaaa tagaaaaaga tatgcttttg aagcaaatta
     7981 agaaatatgg agaagaaaaa ggatttaaat taaaacagag taaaatggga atagttttta
     8041 tccctttgaa agaagaggaa gatgaaagtg atgagtctga tgaagagttt tacaaagcta
     8101 aaagagaact agaaaatatg gcgatacagg tcgtatataa aattagaaac ttagaagaaa
     8161 tagctgaaaa agcagtgtta gagcttgaag aagagatagc taaacttgta gtagaacctc
     8221 atatcaagag attgtgtgaa aaatatgaaa attatgataa gattcaaact tatctggaaa
     8281 atataaagaa agatataata gaatatatgt atttattcta tttagatgaa gaggagttaa
     8341 aagataagta tgataaagaa cattttatta aatataaaat taacctattt gtagacaatg
     8401 gaagctcaaa gaataaagaa tcagcacctg ttgtagtaga gataaaccca agtccagcaa
     8461 atctatttgg aaaggcagag tatgattatg caaatggaaa tataaagact gatttcacaa
     8521 aactattatc aggagcattt catagggcta atggtggtta tttagtactt tatgcagacc
     8581 aattactaaa atatacaatg tcttgggaaa ttcttaaaaa gactttgcag actaaaaaag
     8641 taatattgga aacacaaaca gcaattaaac cagaaaatat gccattggat gttaaattag
     8701 tactgattgg aagccattat atatatgata ttttatatag atatgatgaa gattttgaaa
     8761 aatattttaa agtatttgta gactttgata gtgaaatgga taaaaatgaa gataatgaag
     8821 aaggaatagc aagatttata gcataccaat gtgataagaa taatttaagg cattttacta
     8881 aatcagcagt tgaagaggtt attaaattta gcactagatt atctggtgat ttagaaaaac
     8941 tatcaactaa gtttaataaa ataatggaag ttgtaataga aggaagtgct tatgcagagt
     9001 ttagaaattc ggagtataca gagcagtgtg atgttaaaaa agcaataagt gaaaagagaa
     9061 aaagaatcaa tagagtggaa acacatatgg atgaatcaat tgaaaatgga tttactctaa
     9121 tagaaacaga gggaagaaaa gtaggcgtga taaatggtct gtcagtctta agtacaggag
     9181 aatactcttt tggtagagct tcaagaatta cagcgaccac tagcccagga agtaaaggca
     9241 tagtaaatat agaaagagaa gtaaatatga gtggctctat acataacaaa ggagtactta
     9301 tattaggtgg ctatctttct gagaattttg cacaggattt actattgtca ctaaatgcat
     9361 atgtttgttt tgaacaaaat tatggtggaa tagatggaga tagtgcatct tctgcagaat
     9421 tatatgtact attatcatct cttagtggag tatcaattaa acaaaatatt gctgttacag
     9481 gttctataaa tcaaaagggt gatatacaag tagttggtgg tataagtgaa aagatagaag
     9541 gattttattc tatatgtaaa actaaagggt taaatggtga gcaaggtgtc ataattccta
     9601 gaaaaaatca aagaaatctc gttttatcag atgaagttaa tgatgcagtg cgagatggaa
     9661 aatttaagat atatactgtt gaaagagtag aagaggctat agagatattg acagatgtta
     9721 aatttgaaga aataaagaaa ttggtaaaag aaaaattaat atctttcagc aagatacaaa
     9781 ctgtgtcaaa agaataagtt tattatagaa tttaatgggt atttatgtat aataaaaagc
     9841 aagaacttta ataggttttt gctttttttg taaatatata taaattaaat tcttattttg
     9901 tatgttttat atattgatgt ttattttttg ttgatatgat taaactattt ataaatagcg
     9961 ttttataaat tagtatttta aagggggata attatgaaaa atgtaaatct aaaagagata
    10021 aatgaaaaag atgtaattgt ggaagaagat ataaatctaa aaggaatgga taagaaagat
    10081 gtaattgaga aggaagaagt aaataaaaaa agtatagata agaaagatat aattgagaaa
    10141 gaagagatag ataaaaaaaa tgtaaagaag gtcgaggttc aaaaaggaat agataaaatg
    10201 attggtaaaa aagatgagat aattgatgag aaagatatag agaattttat ggatattgaa
    10261 gaagataaaa aaagtatcaa agtaagtaaa aaaagtacta aaaaaggtga aacggagaaa
    10321 aaaacaaaga agagtagttc taataaggct acacaaaaaa caagtaaaga aactaaaagt
    10381 aagtctaaaa ttaaaaatga gtcagcaaat gaaaaagacg taaataagat tttagacgag
    10441 ttagaaaaat tgtatccaga tgctaaatgt gagttaaatt atgggacagc ctttgaactt
    10501 ttaatagcaa ctattttatc agcacaatgt actgatgtaa gagtgaataa agttacttca
    10561 gagttgttta aaaagtacaa cacagctaga gactttgcta atttgagcat tgaagaaatt
    10621 agtaaagaga taaagagttg tggtctttac aaaagcaagt ctcaaaaaat aaaggataca
    10681 tctgaacagc tatgtgaatt atatgatggt gaagtacctg atagcttaga aaagcttata
    10741 aaattacctg gtgttggaag aaaaacggca ggggttgtac ttagtaacgc ttttaatcat
    10801 ccagcaatag ctgtagatac tcatgtattt agagttagca atagaatagg aatagtggac
    10861 gagccaaatc ctcaaaaaac agaatttgca cttatggaaa ctataccaaa agaaagatgg
    10921 tcacactcac atcatgtatt aatttttcat ggaagaagaa tgtgtaaagc tagaaatcca
    10981 gaatgtgcaa gttgcccaat aaaagaagat tgtaattatt ataaagaact taatgaaacg
    11041 aaataacttt tagaaagtaa gttgtacaag agggattttc tgatataata atacttgtga
    11101 aaatgttaga taaaaaacta ttatatacat gaaataaaaa ttattattaa cattatttta
    11161 tataatagta taatattatt gttattctgt ttataaaata aaatttgata aataaaagca
    11221 atacttaggg aggattaaaa tgtcattaaa tatagtatta gtagaaccag aaatacctca
    11281 aaatacagga aatataataa gaacctgtgc agcaacaggt tcaactttac atttagtaaa
    11341 accactgggt ttttcattgg atgataaaca cctaaaaaga tgtgggcttg actattggga
    11401 tatagctgat atacaatatt acgatagctt tgaagaatta caagaaaagt atccaaatag
    11461 taaatacttt ttttctacaa caaaggctaa acagtcacat tcagatgtga aatatgaaga
    11521 aaattgtttt atagtatttg ggaaagaaac aaagggatta ccagaacctt tgttgaaagc
    11581 aaatatggat acttgtatta gagtgcctat gttggatgtt gaaaaagcta ggtcattaaa
    11641 tttatcaaat tcagttgcta ttgtagtgta cgaggcttta agacaaatag gatacccaaa
    11701 tatgaggtag ttttaacaag cctaaatgag aaaggagcat acttaatgaa aaatctaaag
    11761 ataatctgtg acagtttatc ggacgtgcca aaagatttgt tagataaata tgatatagat
    11821 atagtttcat taacagttat actggaggaa agagaataca aggatagaat tgatatatct
    11881 ggagatgaat tttataaaag gcttagagaa gaaaatgtat atccaaaaac ttctcaagct
    11941 acttatgccc aatttaagga aatttttgag aaatatacaa atgaggatag agagatactt
    12001 tatatagcag ggtctgccac agctacaggt acgtatcaaa gtgctgtaat ggcaaagaat
    12061 gatgtagatg gtaaaatata tacatatgac tctaacttgc tttgtatggg aactggttta
    12121 cttgtaataa aagcaggaga acttgcaagt gaaggaaagt ctgttgctga aatattgcct
    12181 gttttggatg aattaaaaga gaaggctttt gttatatttt cagtagatac attggaatat
    12241 ttacaaaagg gaggtagaat atcatctact aaggcagcaa taggaagtat attaaatata
    12301 aaaccgattc ttgaaataag agatggatta gtttcgcaaa tatctcaagt aagaggaaag
    12361 aagaatgtaa tttctaagat gatggaattg ataaaatcaa attgtggtga tgatttatca
    12421 gaccaaattg tgtatatagg atatagtgat gattttaagg aaaaggaaca attatctaag
    12481 gttatgcaag atgaattaag acctaaagaa attagatttt tccaaattgg tgtgtgtata
    12541 ggtgcacatg ctggtcctgg tgttactggt atcatatgct taaaaaataa ataatatatt
    12601 tttggatgac attatttatt tttttattaa attaggatag aatattaatt attgagatgt
    12661 taaattggat ttattttgga tgaaattaag tgttgatata ttagatttag tttgtaggga
    12721 agtgtgtaaa tagataagaa tctattgcac acttttttat agtaagattt attaaaaaaa
    12781 tttcaaaatg tacttgtaaa ttgtatataa aaaaattata aataggatgt aaaagtaaat
    12841 aggatgtaaa agtaaatagg atgtaaaagt aaataggatg taaaagtaaa taggatgtaa
    12901 aagtaaatag gatataaaaa tatcgtaaat gtatgaggag tgaaatttaa tgcgtttcaa
    12961 aagaatattg atattgatgt taactgtagc aatgatagct ggaatgttgg ttgggtgtgc
    13021 taataaccca agcaataatc ctagctctag caaagataat aaaagtgata taagcgataa
    13081 aagtaattca aaaaaatctg tatcaatgat actagatatt gaaggaacaa ataatgaagc
    13141 tatgaataat tcagctttat tagcattaaa caatgctcaa aaaaaactta atatagatac
    13201 aaacaaagtg gaatcagatg attcctcaac tttttctaat agtatagata ttttatgtaa
    13261 tgacaattac gatttaatta tagctgtagg agctagattt gcaaaaccat tggagatggt
    13321 agccaagaaa tatccaaagc aacaatttgc aattatagac tatgagtatg acaagcaacc
    13381 ttctaatata acttcaattt cttatgaaga taataaatct ggatacctag ctggattaat
    13441 tgctggaaaa atgactgaaa gcgataaggt tggttttata ggaggaacaa agagttctag
    13501 tagggataaa tttgaatcag gttttagaga gggtgttaaa ttttctaata gtagtataag
    13561 ggatatttca gttgagtatg cagatgtttt taaagatagc aaatcagtag aatctatagc
    13621 taaaaaaatg atggataatg gagtggatat tattttttct actactgaag atgattcaaa
    13681 agctgtaata gatgcagtaa gggcaaaaaa taaaaaagtt atagcaacga ataaagatca
    13741 acatgagtta gctccagaaa atgttataag ttctgttgta aaagattttg agaatcctac
    13801 ttataatctg atacaaagct ttgtaaaagg aaattataaa ggcggaaaag ttatagaaaa
    13861 cacagttaag tctggaagta ctgggcttgc ccaaaatagt tctcaaaata ttcctccaga
    13921 tgtgttagaa tatgttaata agaacaataa ataatctact ataatatcca agaatactta
    13981 tgactttagt tatgaggtga aatggatgtt ataatagaca tatatagaaa gacatatgca
    14041 gaggtaggtt agacctatct aaatcaggga gtgaattttg tgaaaataaa aaggatattg
    14101 atggcttgct ttgtagtagt ttttacaatt tttatattag ctatatgtaa ttttgatttt
    14161 ggatttgata aagtaaataa gaataattct aatatagata gtgctaaatc gaaacagaat
    14221 gagtctaaaa gtttgccaac ttcagcagaa aaggataaat caaatagcaa aagtaagaat
    14281 aaagaagatg acacagaaga tgattcatat gtaagaaaaa ctattggagt attgttagat
    14341 accgaaaact taaataacga atcatataat aacaatgtac tatcagcctt aaaaagggct
    14401 gacgaagatt ttgatgttag gataaaactt gaaaaaccaa agacttctaa aagttttaaa
    14461 gagagtatag aaaaactgca taagtatgaa ccagatttga taatagcagt tggagccaga
    14521 tttgcagagc caattaaaga agctgctact aaatatccca aacagcaatt tgcaatagtt
    14581 gactatactt ataaagttca gccagctaat gtaatttctg tatcatttca agataatgtt
    14641 actggatatt tagcaggact tattgcaggt aaaatgactg atactgggaa agtcggtttt
    14701 ataggaggag ttgagggttc tagtagagat aagtatgaat ctggatttag aaaggggctt
    14761 aaaacttcaa ataaagatgc tgagttacta gttaagtatt caaatatatt tgaaaaccct
    14821 gaatcagcta gaactattgc aaaggaaatg agagattctg atagagatgt tgtattttca
    14881 gctacagagg atgatagcaa aagagctatt caggtagcaa gagaaaatgg tggtaaagtt
    14941 atagctatta ataaagacca atacgatatg gctccagata atgtaataag ttcaattata
    15001 aaagatgtag aacaacctgt atatgaactg ataaagaacg ttgcaaaaga tggttttaag
    15061 ggaagtaaag taatggaatt taaaataaaa gatggagaac ttggcttgac ttcaaagagc
    15121 agtagaaata taccaccaga tgttttggaa tacataaaaa aagagtataa caaagtgaaa
    15181 gatacatatt aattattgat gtagaagatt ataaaaatat actgcttaag tttagattgg
    15241 tgataaaagg atatatttat taaatttagt ctaactgtac aaaatatatt gattgagttt
    15301 aggttagcaa taaaaagata tatttattaa atttgtgcta actgtacgga atatactgct
    15361 taagtttaga ttagtgataa aaagatatat ttattaaatt tgaaccaact gtacaaagct
    15421 atactaatta agcttagatt aactatataa aattataaca ataagtgtgt gggcagaaaa
    15481 attctgctac acactttatt tatattcaca taaaaaattt atcaaaaaaa agaaaaagga
    15541 gtaaaaaatg gaatttacaa gagaaccatt tatactagat gacgatttgt gtctagcttt
    15601 ggtggacaaa agaattagtt atctaatgga aaatgaatta aaattaagaa atatagagat
    15661 aataaaaaca atagaatgta aagaattata tgaaagcata aaatatcacc cggatatatg
    15721 tatttgtaat ttaggaaaag gagatataat tgttgcgccg aatgtatata atcaatacaa
    15781 agagttatta agtagatata aatttaatat aattaaggga gaagctaatt tattaaatag
    15841 atacccttat gatatacctt acaatattgc gatagttggt gactatgcta tacacaattt
    15901 taagtatact gacaaagcaa tattagatta tattgaaaat aataaactta aaaagataaa
    15961 tatagagcag ggctattcta aatgctcaat ctgtatagta gatagaacgt ctataataac
    16021 atcagataaa ggtatttgga aaagtatgaa gaatacagaa atagagtgtt tattgataga
    16081 aaaaggtcac ataaatttat ttgagatgaa ttatggattt ataggtggat gtacagggtt
    16141 gatttctaag gataaactag ctttttgtgg agatgttaaa aaacatcctg actatgaacg
    16201 tataaaaagt tttgtagaaa gtaaaaacaa ggagatagtt acacttagct gtgaaaattt
    16261 actagatata ggttctatag ttcctctcat gacaagaaag gagcgataac tttggttgag
    16321 aaggataata tttcttccat gaaatttttg aggaataata atgtaaacga tagattaata
    16381 tatacgaatg acattgtaga agtagtgata gattctgcaa agcaagaagt gttaaataaa
    16441 aaaatactta atggtactaa aactgaacaa agagctttaa atattgatga gatatcaacg
    16501 gaaataacta gtatgataat aagtataatg aaagagaaac ttttgaaaga atacattcta
    16561 aaatgctatg ggcgtacata tcctgaagat aaaaataata tatacatata ttcactgaac
    16621 atatttgcta aaaaagagat ttttatgaga gaaacagtat ttactagggt gagaaattat
    16681 atagaagaaa atgattttat aaatgtagaa ggttttatta gatttagaat gagagaattt
    16741 atgaaatata tttctgctat aggtgatatt gcagttgagg agtatttaat aaagaaagac
    16801 caagatgaat ttataagagt tttaaaatac tttatagata ctcaagaaga aaagattgat
    16861 ttgcttaaag ttcatataat ggaagataat acatttgttt tatacgataa aaatggaaat
    16921 aagatagaca gtatagatga tgaagaaatt ataaatatgg taataagaga aaatttaaat
    16981 tatgaggact ttttaataag taccttatta actctatgtc ccaaaaaaat agaaatattg
    17041 gatttgttaa ataataattg ctcaaaggaa attatagata ctgtagaagc aatttttgaa
    17101 aataaagtta gtataattat ggaaaactag aatctaagca tataaaagct atccatataa
    17161 aattaaaaaa attcttttta aatcgacaca aaagacttat aattgtatat aaagctatag
    17221 tataactagt gttattaagt ataaagaata tctacttgat taatacaata tagctggcaa
    17281 atatacaatt gataaggagt aattttataa tggataattt aattaggata ctgatattag
    17341 ctgttattgg tggatttata ggttatgtta cgaatgtagt tgctataaga ctgatattta
    17401 gaccaataga gcctattaaa atacctattt taaatataga aatagtaggt cttataccaa
    17461 agagaagagc agaaatagca gcaaatgtag gagaaataat tcaagaagaa tttctttcta
    17521 tggacgaaat acttgcaaat atcattactg acgaagataa ggaagaagtt gtaagataca
    17581 taaaagcaag ggtaaaaatt ataatacatg agaaagtttc atttatcccg agtggtataa
    17641 aaaacatgat acaagattat ctaggagaga taattgagtc tgaagttaag caaagtatag
    17701 atgaattgag taaaaatata ataaataaag caaatgaacg tatagatata caaaaaatgg
    17761 tcgaagataa gattaatgaa ctagatttat atgaattgga agagataata ataagaatag
    17821 ctaagaaaga attaaaacat atagagtttt tggggcttgt cttagggttt ttaataggta
    17881 tagctcaagg gttaattact atgtttattt aaatatatga gaagtagcat ttatttaagt
    17941 actaattata tttacctatt tgagtgaatt acttattaat agcatttaga tattaattgt
    18001 atttatttat ttgattgtgt tacttattaa tagtatttaa gtatttactg tactacttat
    18061 taactatatt aagtattgat tgtatttaag tatacaaaac ctaaaaagag tacaaaaatt
    18121 agaagttagt tttaataaaa attagtttta gtatttatct agtcatatct atcatgcatg
    18181 tggatacgtt ttaaaaattt taaatttatg aaaatattat aatatcacta taaatcaaga
    18241 gattaattta atataaaaat ataagaatat tggtttacag ctgattgaga tgaaaaataa
    18301 tgggtaatta atatattaca ttaatgttga ttgatgtata actatctgtt ataatgaaaa
    18361 tatattaaaa atcatagtgt tgaagaggaa gagtagagta gaaaccattc tcagagaatt
    18421 tgggtctatg gctgagagcc catgtaatag gtctatttga aaactacctt ggagctgagc
    18481 ttattagcag tatttgtttt gtactatacg tgaaagtgct cgggtgacga ccttatagtc
    18541 atcaagaggg ttgtatatac ttgtatacgc caacttgggt ggaaccgcga attaatatcg
    18601 tcccaaacat atttggggcg attttttatt acaaaaaatc taaaaattat ttcaataagg
    18661 aggaaaaaac aatgattaaa gttgctttaa aagatggctc aataaaagaa tttgaaaatg
    18721 ctatttctgt tatggatgta gctaaatcca ttagtgaagg attagctaga aatgtggtag
    18781 cagcgtctgt gaacggagag gttgtaggtc tggaccacat tatagatact gattgtgact
    18841 tgaatttatt taagtttgaa gataaagaag gtaaggaagt ttttagacat acatcagcgc
    18901 atatattagc tcaagctata aaaagacttt acccagaagc aaagcttgct ataggaccaa
    18961 gtatagaaaa tggattctac tatgatatag atttagacca tagattagtt ccagaggacc
    19021 tagagaaaat agaagctgaa atgaaaaaaa tagctaaaga agatttaaaa atagaaagat
    19081 ttgaacttcc tagaaatgaa gctttagagc ttatgaaaga acaaggagaa gactataagg
    19141 tagaacttat atctgattta cctgaaagtg aaataatatc tttctacaag caaggtgatt
    19201 ttactgattt atgtagagga ccacatttac catctactaa aaaggtaaaa gctgttaaat
    19261 tacaaagtgt agcaggtgca tattggcgtg gtgacgaaaa taataagatg cttcaaagaa
    19321 tatatggaac ttcttttgag aaaaacaaag acttagaaga gtacttacat ttactagaag
    19381 aagctaaaaa gagagaccac agaaaattag gtaaagaatt aggattattt atgatacctg
    19441 aagaaggtcc aggattccct atgtttttac caaaaggtat ggaacttaaa aatgaattat
    19501 taaaattctg gagagaaata cacagaaaag ctggatatat tgagatagaa tctccaatca
    19561 tattaaatag aaaattatgg gagacttcag gacactggta tcattacaaa gaaaatatgt
    19621 atacagttaa aatagatgat gaggattatg ctataaaacc tatgaactgt cctggtggat
    19681 taatttacta taactctcaa ctacattcat atagagattt cccaatgaga gttgcagagc
    19741 ttggtagagt tcatagacat gagttatctg gtgcattaca aggattaatg agagttagag
    19801 catttactca agacgattca catatattca tgttaccaga gcaaattaag gacgaaataa
    19861 aaggagtagc taacttaata gatggtatat acaaaacttt tggatttgaa tataatttag
    19921 aattatctac aagaccagag aactctatgg gaagcgacga agagtgggaa gcagcagaaa
    19981 atggattaag agaagcttta gaagagttag gtctaccata tactattaat gagggtgatg
    20041 gagcattcta tggtcctaag atagacttcc atctaaaaga ttgtttagga agaacttggc
    20101 aatgtggtac tatccaatta gatatgcaac ttccaagaca atttgataat acttatattg
    20161 gtcaagatgg tgaaaagcat agaccagtta tgattcatag ggttgctttt ggtagtatag
    20221 aaagatttat aggtatatta atagagcatt atgctggtaa attcccagtt tggttatctc
    20281 caactcaagt taagatactt cctatatctg ataaatttat ggattatgca aatgaagtta
    20341 agaaagagtt atttgataag ggtataagag ttgaattaga tgatagagct gaaaaaattg
    20401 gatttaagat aagagaagct cagcttgaaa aagtgccata catgttaata gtaggtgaaa
    20461 aagaagtagc tgataataat gtttctgtta gatcaagaga taaaggtgaa ataggtagta
    20521 ttaaattaga tgaatttata gctagtattt caaaagaaat tgaatcaaga gaaagtatta
    20581 ttcaagacta atatatatta ttttagtagc taagatattg ttatataaga taagtttaat
    20641 caatatgttg atatgttgat gcatagtgat taaatttatg aaaagatgag tataattaga
    20701 ggtacagtta atcatatttt tatgattaat gtacctctag ttttttgtat ggtagattta
    20761 ttaataaata tatcatagaa gtgttttgaa catggaaata tttatgtgca ttctttaaat
    20821 aaaagtttat tttttgagtg ataattaagt gcttgttggt aaggttatgg ttgtaaaaat
    20881 cataacttta ttttcatatt tgttccattt tcataaaaat tattatccct ttaagaaaat
    20941 ttcttaatcc tcttgaaata tttccttttt agtctaaagt taaatttctt tcttcaaaat
    21001 gaatatttaa atgaactaaa atttaagtac attttctaat cttatgagtc tatttttcat
    21061 agataattgc cctataatag aagaatctat gttggagcta tgtggtgaca ataacagtat
    21121 tgtcagtgat taattaatgc tttataaatg attaattagt gttttatgtt atttttggac
    21181 ttaccctcta tacatagatt aatttattta actttagata gagagagaat gtcaagtaca
    21241 tacacagaca ttagagttta agaaagtgtt tatggagcaa gaacttttaa aagaggtagt
    21301 gacttagttt aatttcaaga aatgataaat acaaaagagg aaaaagagag gtgttgaaat
    21361 gatagaaaaa gtagaagaaa aatctaagat tattaaaaca tcaaaagaat ataagcgaac
    21421 tataattgaa gaaaaagata aagaatggac caaaaaatta aatgatatac ttagtagaag
    21481 tagtttttct aatgatgaat tagataaaat caaggcgtta attccaaaag agattttgac
    21541 taatgaaaat gttggagaag ttgtgactga agatggatat gctaagcccg agtatgatga
    21601 ggtatttaaa tcattgttca ctttaaataa tgaatatgat ttacttgtaa attttataaa
    21661 tgatatccta aaagatgctc catatgctaa caggaatatc aaaccgttca ctcaaataaa
    21721 gagaataatt agagttgaaa ctgacccaac aattaattac attggagaga aaagacctag
    21781 acttgatatt ttggctgaag atgaagaaaa taatcatata aatattgaga tgcaaagagc
    21841 ttttgagaac gattatttgg aaagagctga atattatctt agtcgtgtac atggtagaaa
    21901 gttagaagaa ggtaaagagt ataaagaaat tggaaaaact attggaatac atatactaaa
    21961 tcatgtaaaa tataatcaca tagaagatta tgtaaattgc ctgagattaa caatggacgg
    22021 gcatccagat atttattcta gtaaaactgc attatatttt atagagcttc caaaaattca
    22081 taaatctgac tatatagtaa atagaattat gctatgggga aaacttatta gtaatccttc
    22141 tagcttagat gtaagagtta tagctaagaa tgattctatt attaaaaaag cacttgatag
    22201 attaaaagaa ttaggtagca atgaagaata tttattaaat ctgaaaagag gtgcttatat
    22261 catgaataaa agtagaaatt ttaaagaaga gattgaaaga gagacagcaa ttagagttgc
    22321 aaaagaaaaa gattataaaa taattataat gttgaaaaaa aatggtttta aattagatga
    22381 catattaaat aaatttaatg attttgactt aagtgaagat gagataaaag aagtatatga
    22441 aaataattga tgataaaaag tgaacttata taagtttgct gaatcggagt ctggaaatat
    22501 ttatgtgtat tttttaatga ataaaaattt attttaaaaa caataattta gaggtacagt
    22561 taattatatt tttatgatac tgtacttcta gttttttcta ttgtagattt attatttact
    22621 aataaatcta caatagaagt gcttttgttg taagaatcat tttatctaat gattgttttt
    22681 tataatagtt aaatttccat ttgtagtgtt taaatataaa tctataacag catttctata
    22741 aatgttataa atcactataa tgattcaact caaagttact gcttaaagaa gcagagattt
    22801 tatcaaggta tatcatgga
//
LOCUS       DAGSRM010000060        22316 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.060, whole genome shotgun sequence.
ACCESSION   DAGSRM010000060 DAGSRM010000000
VERSION     DAGSRM010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22316
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.060"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            107..898
                     /locus_tag="MJE33_002423"
     CDS             107..898
                     /locus_tag="MJE33_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HBY9648355.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALMAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVDKDIILLGLYNSEMINSAKEIGLR
                     FANEVFADRAYDNNGFLVPRNVEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELEGIEVCPLENIEVCSSENIV"
     gene            927..2147
                     /locus_tag="MJE33_002424"
     CDS             927..2147
                     /locus_tag="MJE33_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HBY9648356.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVKPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVATMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIIAFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            2167..2862
                     /gene="pxpB"
                     /locus_tag="MJE33_002425"
     CDS             2167..2862
                     /gene="pxpB"
                     /locus_tag="MJE33_002425"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HBY9648357.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGI
                     VTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            2892..3878
                     /locus_tag="MJE33_002426"
     CDS             2892..3878
                     /locus_tag="MJE33_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase"
                     /protein_id="HBY9648358.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGARAYVSIAGGIDVPLAMGSKSTYTRAKIGGFEGRALKKGDYINTFIQEKDF
                     TINKKLSSKYIPTYSSEIVLRIVKGPQFDAFSNGEVEKFLSNKYKVTNEIDRMGCRLD
                     GESIKHLNGADIISDGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLNDIAV"
     gene            complement(4073..4639)
                     /locus_tag="MJE33_002427"
     CDS             complement(4073..4639)
                     /locus_tag="MJE33_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY9648359.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTTEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDICMGEKVVRLNPGDSTFIKANVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
     gene            4991..6058
                     /locus_tag="MJE33_002428"
     CDS             4991..6058
                     /locus_tag="MJE33_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HBY9648360.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESIILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNAPLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNTKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEELEGISGGF
                     VMDGDIMNLVNKPGLGIEVNL"
     gene            6077..7237
                     /locus_tag="MJE33_002429"
     CDS             6077..7237
                     /locus_tag="MJE33_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBY9648361.1"
                     /translation="MNTLFSNPGSILAVMTSMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPISMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEESELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVSTILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFASFVFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAIKMSLGL"
     gene            7250..9346
                     /locus_tag="MJE33_002430"
     CDS             7250..9346
                     /locus_tag="MJE33_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HBY9648362.1"
                     /translation="MIKFKLYISGLYSGNVIFDGDLLIEKLNPFTNKIESLKPISKEE
                     NTYYLNLTKIDLKSLFNNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWVQRNKKFP
                     LDIIIVDNKIVGFICLSRETCTILIMDGYEEYTVLKEWEKTHKNEEIYSIRFGGNYMI
                     DMKDGIKLSTDVYLPDFVDSAKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVR
                     GRNESEGKWEPMIHEREDGDSTINWIVSQEWSSGIVGMLGASYLGYVQWAAASSGNKH
                     LKALVSIVTSGSPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRP
                     IENIPVEALGYRIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPALVVSGWYDDNSMG
                     TTEALDVIKDYEKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDKY
                     LKGIENNIDTTAPVEYYSVGSNKWKTEENWPIINKIDKSMYLISDGNANTSLGNGRLV
                     FDNDLEEKYDSYIYNPKDPSVQLIDMSENEVGVPNNYKDLEKRSDMLCYTSDAFSEEF
                     TVTGDIKLEFFASSSAKDTDWVIKIMDVDLDGNSIKLADGILSARFRNSFYKSEFMEE
                     GEIYKFTIITSKISNTFKVGHKIRLDITSSAKNFIFQNSNTTEGYNSIEYIEAKNTIY
                     HGGKYPSKLILPIENK"
     gene            complement(9505..9849)
                     /locus_tag="MJE33_002431"
     CDS             complement(9505..9849)
                     /locus_tag="MJE33_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBY9648363.1"
                     /translation="MEIKRYEGTGRMSRAVVHNNTVYLCGQTHAEGGVIEQTTEVLAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
     gene            10141..11031
                     /locus_tag="MJE33_002432"
     CDS             10141..11031
                     /locus_tag="MJE33_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBY9648364.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWLIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEVIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            11249..12535
                     /locus_tag="MJE33_002433"
     CDS             11249..12535
                     /locus_tag="MJE33_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBY9648365.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDVCEIVAVAEPKKGRRE
                     LFTQKYNLDSKNVFETLEDFFKHDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHIDDLCDKYKDKIFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTEENLLNILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKEFGKNEEIVMNPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDSAKQMTKEIEETIF"
     gene            complement(12624..13067)
                     /locus_tag="MJE33_002434"
     CDS             complement(12624..13067)
                     /locus_tag="MJE33_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory YrvL family protein"
                     /protein_id="HBY9648366.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFGITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDRDKKKP"
     gene            complement(13091..13885)
                     /locus_tag="MJE33_002435"
     CDS             complement(13091..13885)
                     /locus_tag="MJE33_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HBY9648367.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEITAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKKIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
     gene            complement(14451..14927)
                     /locus_tag="MJE33_002436"
     CDS             complement(14451..14927)
                     /locus_tag="MJE33_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBY9648368.1"
                     /translation="MKEILINPIGKICIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDNPELRSILETPKPYKSGPDVIGIFATRSPIRPNPIALTAVQIINIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSVDRVENPSVPKWCSHWPKSVEKSGEFNWEDEIEF"
     gene            complement(15028..16197)
                     /locus_tag="MJE33_002437"
     CDS             complement(15028..16197)
                     /locus_tag="MJE33_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBY9648369.1"
                     /translation="MLNIKTLCHEINDWVINIRRDLHKTPELGLEEFQTKKKIIKYLN
                     EIGINYIEYKNHTGITAYINVSPNFETVAIRADIDALPITEELNYSYKSINIGKMHAC
                     GHDAHTAILLGTCNILFKLKDYLNVNVKFFFQPAEETIGGAQLMIEDGCLEKPNVKYI
                     FGLHVNPNINKNLIELKYNTLNASTNTLQLTVHGSKCHGAYPHQGIDAIVISAHIITA
                     LQTIVSRNTNPTDSVVISLGKIEGGIKENIVCDKVVIRGTLRTLTPETREFSKKRIRE
                     ICDFTCKTFGGSISVEIEEGYPALINSNHLVDYVKQNAVELFGEENIILKDSPTLGAE
                     DFSYFLRHCEGAFYHLGCANREKNITSPLHTSTFDIDEDCLITGVMLHVKNVLSF"
     gene            16360..17223
                     /locus_tag="MJE33_002438"
     CDS             16360..17223
                     /locus_tag="MJE33_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBY9648370.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYL
                     AGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVGKWGKFTYKSLVNALEFKDELYLENPKEEKIYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFKDCIEENDLMELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
     gene            17294..17887
                     /locus_tag="MJE33_002439"
     CDS             17294..17887
                     /locus_tag="MJE33_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HBY9648371.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
     gene            complement(18138..18773)
                     /locus_tag="MJE33_002440"
     CDS             complement(18138..18773)
                     /locus_tag="MJE33_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBY9648372.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAR
                     QGALYSPRVESLIKPIEMFTKKISNKLNRVIAFTDSHTPKSIELLSYPVHCLENDVES
                     ELVDELKSIENLQILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNVHPAEILNLVFFNSMIQNGVNVLKEIR"
     gene            complement(18853..19584)
                     /locus_tag="MJE33_002441"
     CDS             complement(18853..19584)
                     /locus_tag="MJE33_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBY9648373.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLDMDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLRKLKTLNSSFEGYQSNKECKIPTLEEVLILISPTD
                     LTLNIELKTDKINYPNIEKGVLELILKYNMKNRVLISSFNSNSLKNFHKLDPSVKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIELCHKNLLGVNVYTVNEEDDILHCL
                     KLNVDGIFTNYPDIASNLLHSKQYS"
     gene            19915..22104
                     /locus_tag="MJE33_002442"
     CDS             19915..22104
                     /locus_tag="MJE33_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HBY9648374.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKIPNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTMNIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EKKGYDLSVIVYTKRALKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDNDILIEDL
                     EFVCKEEEKVLLEDFNNNQYEVPYDKSYVDLFREQVKVTPSNIAARDENRKLTYKELD
                     EVTDKLAGYLNHIGVESEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLN
                     YLLEDSNAKVVITTDELKSKVVNENTIVLDINDEEMFKFGYELTEKITSSNCAYTIST
                     SGSTGRPKTIAIEHKSLVNMCYYSVKSISATENDICGIYLSFSFDAVMKQLFPYLLVG
                     ASIDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERL
                     KGYRKRNYELYNEYGPTEFTVVSTSFHVDREYGKIPIGKPIFNTYAYVLDKKNKLCPI
                     GAPGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEHNKLMYRTGDLVRWLDDGNLD
                     YIGRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDDGDIYICAYYVIDEED
                     SGKINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKRALPE"
BASE COUNT         8532 a         2559 c         3750 g         7475 t
ORIGIN      
        1 aattgaatac aagtgataaa atatttcatg caatatataa aataaacaaa atttaaaaat
       61 taaaactact tattctatca aaatgttagc gaggaggggt cataaattgt ataaagtaga
      121 tttaaatagt gatttaggag aaagttttgg aacgtataag attggtttag atgaagaagt
      181 tttaaaatat atttcatcag ccaatatagc ttgtgggttt catgcaggtg accctagtca
      241 tatggaaaaa acagtacagc tagctaaaaa aaatggagtg aaaataggcg cacatccagg
      301 tttcctagat ttaataggat ttggaagacg tgagatgaaa ataacaaagc aggaagctaa
      361 agattataca aaatatcaac tgggtgcttt aatggcattt gcaagttcaa atggatgtaa
      421 tatacaacat gtaaaaccac atggagcatt atacaatatg gcagctaaag ataaagagtt
      481 agctatgggg atttgtgaag ctatatatga agtcgataaa gatattatac tgttggggct
      541 atataatagt gaaatgataa attctgctaa agaaattgga cttaggtttg caaatgaagt
      601 atttgcagat agagcttatg ataataatgg ttttttggtt ccaagaaatg tagaaggagc
      661 agttatacat gatacaaaac atgcaataga tagggttgtt aggatggtaa aagaaggaac
      721 tgtagaaact ttaacaggtg aagttataca tatcaaagca gattctatat gtgtacatgg
      781 ggacaatcca aaagcaatag agtttgtcaa agaaataaga aagcgttttg aattagaagg
      841 tatagaagtt tgtccattag aaaacataga agtttgttcg tcagagaaca tagtataaga
      901 ctgataacat gagggggaga taaaatatga gtacaaaaaa tattacagat aaaactaaaa
      961 ataaaaaaga tgtcggagca cttatagggg ctgcatttat aatggcaaca tcagcaattg
     1021 gaccagggtt tttaactcag actgcacaat ttacccaaga ttttggaccg aatttttcat
     1081 ttgtaatttt aattacaaca atccttttta taggggctca agtaaatgta tggagagtaa
     1141 ttggtgtatc aggacttaga ggtcaggata tagcaaataa aataatacct ggattaggct
     1201 atttagtagc ttttttagta gctcttggag gattagcttt taatatagga aatgttggtg
     1261 gagctgcact tggtatgaat gtaatgttta atatgaatat gacattagga actgtattaa
     1321 gtggattaat agctatattt gtatttatgt caaaaaattc aaattctcta gtcgataaga
     1381 taactaaatt tttagcatta ggaatgataa ttatagttgg atatgtagca atatcaaatc
     1441 atcctcctgt tggagaggca gtatcaagaa tggtaaaacc agaaaatcca aaaggactga
     1501 tattcccaat aataacttta cttggaggaa gtgttggagg ttatataact tttgctggag
     1561 gacatagatt aatagacggt ggaataacag gagaagaaaa tataaaagaa ataactaaat
     1621 cttctctttt gggtatatta gttgccacaa tgatgagagt tcttttattt ttagcaatac
     1681 tggcagtggt ttcaaaaggt cttcaacttg acccagaaaa tccagcagct tcagctttta
     1741 aatttagtgc aggagcaata ggatataaat tctttggact tgtattatgg tctgctgcaa
     1801 ttacatcagt tataggagct gcttatacat cagtttcttt cttaaagaca ctaaatcctt
     1861 ttatagataa atatgaaaaa tattttataa tagcatttat agcaatatca acacttataa
     1921 tggcatttat aggaaaacca gctactttat taattttagc aggagcttta aatggattga
     1981 tacttccaat cacattagga attatgctta tagcatctaa aagaaaagat attgttggag
     2041 attacaagca tcctacatgg cttttaatat ttggattaat agtagtttta atatctgcat
     2101 atacaggaat aacttctctt agcagcctag gtgctttatt tgcttaattt agaagaggtg
     2161 acatttatgg aaactagata cttgatatca ggggataagg cagtggttgc tgaatttgga
     2221 aatgagatat cagaagatat aaacaaaaaa gtaatttcat ttatgagagc aatagaaata
     2281 tcgaatctta aaggtattgt aacagaaatg gtaccaacat ataggtcact tatgataagt
     2341 tataatccat tagaaataga ttttgatagt ttgattgaga atttaaaaaa aatagaagat
     2401 aatttggaaa gtatagtatt accaaaacct aaaatacatg agattccagt atgttatgat
     2461 gaggtttttg gtatagatat taaaaacgtt gcaagctaca ataatctcac tgtagatgaa
     2521 gttataaaaa tacacacaag cagagagtac ttgatatata tgctaggttt tactcctgga
     2581 tttccatatc taggtggtat ggatgaaaga atagctacac ctagattaga agtgccaagg
     2641 actaaaattt atggtgggag tgttggaata gcaggttccc agactggtgt atatcctatt
     2701 gatagtcctg gtggatggca aataattggt aggacacctt taaaattata tgatgaaaat
     2761 agagaagaac agattttgct tagagctgga gattttataa aatttgttcc aataacatta
     2821 gatgaattta tagaaatcga aaaaaataac ttaagtattt aaatatcgtt atagtacagg
     2881 gagtggtata tatgggattc aaagttgaat taggaggttt ccaaacatta atacaagaca
     2941 gaggtagagt tggctatggt caatatggtg tatctggatg tggagcaatg gatgagtatg
     3001 cacatagagt aggaaatata ttagtaggaa attctgaaga tgaggcttct ttagaagttt
     3061 taatgttagg tcctacaatt acctttgatg aatatacgca aatagcagta actgggggag
     3121 atttaggggc taaaataaat ggaaaagaaa ttcaaaactg gaggtcatat caaataaatc
     3181 caggtgatgt attatcattt agaggtgtga aatcaggtgc tagagcctat gtgtcaatag
     3241 ctggtggaat agatgttcca cttgcaatgg gaagtaagtc aacttataca agagctaaaa
     3301 ttggtggatt tgaaggtaga gcacttaaaa aaggagatta tataaatact tttattcagg
     3361 aaaaagattt tacaataaat aaaaaattaa gttcaaaata tattccaact tatagttcag
     3421 aaatcgtact aagaattgta aaaggtcctc aatttgatgc tttttctaac ggtgaagtag
     3481 agaaattttt atcaaataaa tacaaagtta cgaatgaaat agatagaatg ggttgtagac
     3541 tagatggaga gagtataaag caccttaatg gagcagatat tatatctgat ggtatttctt
     3601 atggagctat acaagtacct ggtcatggaa aaccgattat aatgttatca gatagacaga
     3661 ctagtggtgg ctatactaag atagggaacg ttataagtgt agatttgtat aagttagcac
     3721 aagcaaagcc aaatgatgta gtaaaatttg aacttgtaga tatatatgaa gcacatagac
     3781 tattgagaga gcaagaagat aagattcaag atatctacaa gagtatgaaa aatataagag
     3841 ttgtaaaggc aaaagtatta aatgatatag cagtttaatt ttaacaaata caatttttat
     3901 tataatttat aatataactt acaaatagct atttactatt taccataatt aagatggttt
     3961 agattagtca aatagctatt tttttagttt gagtaaatat tttaaattta aaataaaagc
     4021 aatatttaaa tctattatat tatctacaaa aagtaggtgt gtcatattga gtttatttag
     4081 attttattgg ccaaattgga ggtgatataa ctccatatcc acgagctatt gtatctttac
     4141 ttggattgat gattttatgt ggtacattag cttttataaa tgttgagtca cctggattta
     4201 atctaactac cttttcaccc atacaaatat caaccacacc actcgttacc accataattt
     4261 cttctgatgc atgatatata taatctttgg catcctcact ttctggttta agttcaaact
     4321 ctacaaataa aattgaaggt gtaaactcag ttgtaggcat tggagatact atctcgtaaa
     4381 acatttcact ctttggaaat ttcatcataa ctctatcttc ttttctaatg actaaatcat
     4441 cagtattatt gtcatccata aacatatata aaggagtgtc taaaacttca gcgatttttc
     4501 tcaatgaaga caaggatggt tcaatagcat ctctttctat ttgagagata taacttatag
     4561 ataaatctgt caatttggaa acatcactta atgttttttt cttgcttttt cttatatttc
     4621 tcatcttttc acctagcata agaacctcca aataataaaa ttaatataaa atattcgctt
     4681 aaaacaaagt aattatagca caacatagaa gtagtggcaa taagaataca tagtagattt
     4741 tgacaattaa aatttaatta aatccaaata aaagtctatg aaaatgaatt attgttaaat
     4801 taatacaaag aaaataataa taaaaaaatt taatagaaga gtattaattg aattaagatt
     4861 atcacattat attttggaaa agagtaaatt ttcagtaaat tatcttgaca tatatattga
     4921 agtaataata taatttaaag tataaaattt taaaaattga attataactt caaaaaataa
     4981 ggagggtaat atgaagataa cagatatcaa atttgaaaag cttagaatta aattaaaaaa
     5041 acctgtagta gtttcatttg gagtaattga gtatggagaa agtataattt taaaaattga
     5101 aactgatgaa gggtattgtg gatttggcga agcagcacct cttgcagcag ttacaggtga
     5161 agttttggat aatgttcttt cagtattgct tatgtttaaa aaagagttaa taggcaaaga
     5221 cccactagat attgaaacca tacataccat catggatgga attattatag gtaatacttc
     5281 tgcaaaagca gctattgaca tagctttata tgatattaaa ggaaaaatta tgaatgcacc
     5341 tctttataaa gttcttggag gatttgatag taaagtccaa acggatatta ctataagtat
     5401 agataagcca gaaaaaatgg cagtagaagc tttagaaaga gtaaaagaag gttttaggat
     5461 attgaagctt aaagcaggta taaatcctga agacgatatt gaagctgtaa agttaattag
     5521 agaagccgtt ggagagagta taagaattag aattgatgca aatcaaggat ggaacgtaaa
     5581 tagcagtata aatactatca aaaagttaga agaatttaat gttgatgcaa ttgaacaagc
     5641 acttccacat tgggatttag atggtactgc ttacataaga aataaaagta acacaaaaat
     5701 tatgatagat gaatcattac attcaccaat agatgcaatt aaggctataa aaaagaatgc
     5761 agttgataca tttaatatta agctgatgaa atcaagtgga atatatcctg ctataaaaat
     5821 caacaacata gctgaggcaa gtggagtaaa ttgtatgtta ggatgtatgc tggaaacgag
     5881 aattggtata acagcagcag caaatttaat agcatctaaa aagaacataa cagaagctga
     5941 ccttgatagt tttatgtttt gtgaagaatt agaaggtata tctggaggat ttgtaatgga
     6001 tggagatatt atgaatcttg tcaataaacc aggtttagga atagaagtaa acttatgata
     6061 aatttggagg ttaaatatga atacactttt ttcaaatcca ggtagtatac ttgctgtaat
     6121 gacatcaatg attgcattgg gtttttactt acaaagatac aaagccatta aaagcttagg
     6181 accagcactt acaataatca taatgggaat aatactttca aacttaaaag tagttccagt
     6241 aagtactgag ttatatggaa ctatttcaac atatgctatt ccaatttcta tgactataat
     6301 gttgatgagt gttgacttaa aagaaatgac aaaattatca agagaacctt taattgcaat
     6361 ttttgtagct gtattaactg taagtatcat ggcattttta tttggattag tatttgcaga
     6421 aaaaatatca gaaggttgga aggttgctgg aatgtttgta ggtacatata ctggtggaag
     6481 tgctaatctt acagctattg gaacaggtct taatgtaagt agacaaactc ttgcagcagc
     6541 aaatgcagca gactatgtaa ttggagtacc aacattgata tttatgtttg ctctaccagc
     6601 aatattaaaa aattcaaaga agtttaaaaa actttggcca tatcatgtag aagaatcaga
     6661 attagaagat tgtcaaaatg aagaatttat ggagtcaaaa gaatggagta ttaaagatat
     6721 agcttggatg ttggctatag gttttgttgt tacagaagtt tcaacaatac ttgctggata
     6781 tttcaattca agttttagta gtgcagcaag aatactttta gttacaacaa tttcaattat
     6841 aattgctcaa ttaaagccag taaagaaact aaagggaaat ttagatttag gattatttgt
     6901 tgcattattc tttttatgta caataggttt ttcagtagat ataaaagagt ttttaggttc
     6961 aacttttact ataacacttt actgttttag tataattttt gcttcatttg ttttccattt
     7021 aggcataact agacttttaa agataaagta tcaatatgta atactttcta tagtaggagc
     7081 aattgctgat ggtcctactt cagctttagt agctgcttct gcaaagtgga attcgcttgt
     7141 aagtgtagca gtcgtaatgg gagtaattgg tggagtatta ggaaattatg caggtatatc
     7201 agtggcatat gcaataaaga tgtctctagg attatagggg ggagtttaat tgataaaatt
     7261 taaattatac atatctggac tatacagtgg aaatgtaatc tttgatggag atttattaat
     7321 tgaaaagttg aatcctttta caaataaaat agaaagttta aaaccaataa gcaaagaaga
     7381 aaatacatat tatttaaatc ttacaaaaat agatttaaag tctttattta ataattttga
     7441 tgtatatact aaaagcttag taaacacaga taaagacaca gttataaata acttaggaga
     7501 aacgcattct aaacttaatg aatatatatg ggttcaaaga aacaaaaagt tcccactaga
     7561 tataattatt gtagataata aaatagtagg ttttatctgt ttatctagag aaacttgtac
     7621 tatattaatt atggatggat atgaagagta tactgtatta aaagaatggg aaaaaactca
     7681 taaaaatgaa gagatatatt ctataagatt tggtggaaat tatatgattg atatgaaaga
     7741 tggtataaaa ctttctacag atgtatatct ccctgatttt gtagattctg ccaaaaaagc
     7801 accgacaatt cttatgagaa ctccatatgg aaaagaaaat gataaagaaa tatattataa
     7861 atatgtacaa agaggatatg ctgttgtaat acaagatgtt aggggaagaa atgaatcaga
     7921 aggtaagtgg gaacctatga ttcatgagag ggaagatgga gatagtacca taaattggat
     7981 agtatcacag gaatggtcaa gtggaatagt tggaatgctt ggagcatcat atcttggtta
     8041 tgtccaatgg gcagctgcat cttcaggcaa taagcatctt aaagctttag ttagtatagt
     8101 aacatcagga agccctttta ttgatatacc aagaaaagga ggagcttttg tatctggtat
     8161 gcttgcatgg gcatttatgg tatcaagaaa taaagttgat agaagcaaaa tggtaagaga
     8221 tgattgggat gatgttttaa acatacgacc aatagaaaat atacctgttg aagctcttgg
     8281 atatagaatt gaatttctgg aagaatggtt aaagagagta gaaaaagatg agtattggga
     8341 tttgatggac tggcatttac aaaaagataa aataaatgta cctgcattgg ttgtatctgg
     8401 gtggtatgat gataatagta tgggaactac agaggcctta gatgttataa aggactatga
     8461 aaaaggaaag agaaaggcta ttctaggtcc atggatgcat aattcaaata ctttaagaga
     8521 tataaatggt atttctttag gaaatagttc attaagatat gatttggatt ataattatct
     8581 attatggttt gataaatact taaaaggtat agaaaataac atagatacga ctgctccagt
     8641 agagtattac tctgtaggtt ctaataagtg gaagacagaa gaaaattggc caataataaa
     8701 taagatagat aaaagtatgt atctaataag tgatggaaat gctaacacat ctttaggaaa
     8761 tggtagatta gtatttgata atgatttaga agaaaagtat gatagttata tatataatcc
     8821 aaaagaccca tcagtacaac taattgatat gtcagagaat gaagttggag ttccaaataa
     8881 ctacaaagat ttagaaaaaa gaagtgatat gttgtgttat acatcagatg cttttagtga
     8941 agaatttact gtaacaggtg atataaagtt agagtttttt gcatcaagtt ctgccaaaga
     9001 tactgactgg gttataaaaa ttatggacgt ggatttagat ggaaattcaa ttaaattagc
     9061 tgatggtata cttagtgcta gatttagaaa tagtttttat aagtcagagt ttatggaaga
     9121 aggagagatt tataagttta cgattataac ttcaaaaata tcaaacacgt ttaaagttgg
     9181 tcataaaatt agactagata taacgtctag tgctaaaaac tttattttcc aaaatagcaa
     9241 cacgacagag ggatataata gtattgaata tatagaagca aagaatacga tttaccatgg
     9301 aggtaaatat ccatctaaat tgatattgcc aatagagaat aagtaaattt atatagatta
     9361 aatagtgagg aggtgtctca aaatgaattt tttagttcgt gagacacttt cttttatacg
     9421 aaatcaaata tattttcatc atttcaacaa tgaaaaagag gcttatctct ctgttttgag
     9481 atagcctcct ataagctaaa ttgattattt tgtagctgca acaacgctca tttcaactaa
     9541 caaatgctct ctagcaagtc tagcttcaac acaagctcta gcaggttcaa atcctttttc
     9601 aacccaagca tcccaaacag agttcatagc ttcaaagtct ttcatatctc ttaaataaat
     9661 agtaacagat agcaaatgtt gcttatctga accatatttg tttaataaat cttctatttt
     9721 agctaaaact tcagttgttt gttcaattac tcctccttca gcatgagttt gtccacataa
     9781 ataaactgta ttgttatgaa ctacagctct actcattctt ccagttcctt catatctctt
     9841 tatttccata ttttaaatac ctccatagtt aaaaaatcta ttaaaaatct aaataataaa
     9901 ttaaaaaaag tctatactat ataaaattat attatatatt ctgataataa tccataaaaa
     9961 catataactt tttagctata attattattg ttattaattg aaaagattta ttagttactt
    10021 gacatagttt acacttttaa ttatagtata aattggaata tttagataat tattatttaa
    10081 tgtataagct aagtttttaa ttaatatggc tttatacatt tatatagaaa agaggtacgt
    10141 atgagtagca caatcaaatc aaatcgaata aaaggtataa tgtttataat agcatcagcg
    10201 cttggctttg caatgatgtc tgcattcgta aagctagctg gagatttacc aagttttcaa
    10261 aaagtgttct ttagaaatct ggtatcagct ataatagctt tttggcttat cataaaacat
    10321 aaaggaagtc taactggtaa aaaagaaaat ttaaaagtac ttttatatag gtcaatattt
    10381 ggaactctag gagttatatt taactactat gcaattgaca agttgatgct ttcagatgct
    10441 aatatgctaa ataaaatgag tcccttcctt gtagtaatat tttgtgcaat tgctctaaaa
    10501 gaaaaaataa atgtaaagca gataggagct attataattg catttatagg agctttattc
    10561 ataataaaac caacatttag tgtagaagta attccatatc ttggaggaat tgcaggagct
    10621 gtatttgcag caatggcata tacttgttta agagttcttg gagatagaga agattactat
    10681 acaattgtat ttttcttttc agttttttca ttagttactg tagggccaat tgcttttgca
    10741 gtatatgaac caatgaccct tatgcaactt atatatctat tactaggtgg agtatttgca
    10801 agtctaggtc aatttggaat aactctagca tataaatatg cacctgctaa ggaaatctct
    10861 atatttgact atagtaatat aatcttttca gcaattttaa gtatattctt attcaatgtc
    10921 tatccagata tgttaagtgt tgttggatat ttaatagtat tttcggcagc attttatatg
    10981 tttttataca ataagaagtt agataaatta gacaaagaaa agaagaagta aactagataa
    11041 aagtgttttt ataatttgta tttaatattt aagtctttgt attaaaatta aagaaaattt
    11101 aattaaaaat gtttggtata ttaacttata acagcagtta aaattgaaaa attataactg
    11161 ctgtttcttt tattaaaaat atccaaatgt ctgtaaaaat agactataat agagtagtat
    11221 aaataatata aaggggataa attaaagtat ggaaaaagtg aaagttgctg tgattggagc
    11281 aggtaataga ggaacgtatg catatgctcc atatatttat gaaaattctg atgtatgtga
    11341 aatagttgct gtagcagaac ctaaaaaagg aagacgagaa ctatttacac aaaaatataa
    11401 cctagatagt aaaaatgtat ttgaaacttt agaagacttt tttaaacatg acaagatggc
    11461 agatgcagta ataatagcta caaatgatga tagacattat gatgtagcaa aactagctct
    11521 tgaaaaaggt tatcatgtac tattagaaaa gccaatgtct aatagtttag atggtctggt
    11581 acatatagat gacttgtgtg ataagtataa agataagatt tttatgattt gccatgtact
    11641 gagatattct ccattttata ataaattaaa agaaattgta gaaagcaaaa aattaggaga
    11701 gctagttagt atacaatata atgaaaatat aggatattgg cattttgcac atagctttac
    11761 tagagggaat tggagaaata gcaatgaaac cagcccttta attttagcaa agagttgtca
    11821 tgacatggat attttgttat atttagtagg aagtagatgc aaaaaaatat ctgcatttgg
    11881 aagcttaaag catcttaata accaaaatgc tagtggagag atggcacaaa attgtcttca
    11941 gtgtttagtt gaaaaaaaat gtccatattc agcaaagaga atttatttag aaaaagatag
    12001 aagtataaat agagctgttc acataaatcc aactgaagag aatctattga atatacttaa
    12061 gacaagtcca tatggaagat gtgtttacag atgtgataat aatgttgtag acaatatggt
    12121 aaatatacta cagtttgaaa atggagtgac ggctactttt aatttatgtg catttactaa
    12181 agaaaatgga agaactataa agttaatgtt tagccatggt gaagttggtg gggatttaaa
    12241 taaaaatgag ataagaatta aagaatttgg aaaaaacgaa gaaatagtta tgaatccatc
    12301 aaatcaacag aatatggtag aggaatatga tagaaatctt atagctgaat ttattaaatt
    12361 agtttcaaat aaagaactag aaaagggtag agtagcagca aaagaagcta tacaaagtca
    12421 tgtaatggca tttgcagcag aatattctag ggtcagtgat gaagtagtgt atatagaaga
    12481 attttttgat agtgctaagc aaatgactaa ggagatagaa gaaactatat tttaattaat
    12541 aaaaatttat taaataaaat aaagatagat taaataatag aaatacgaaa atatgtgttc
    12601 tattaaggaa tctatctttt ttattaaggt tttttcttat ccctatcaaa aaaatccata
    12661 cattctgata aaagataaga aagtaaagcg aataagattt ctgtacttaa aggaatcgtt
    12721 actgaatcca taaaggtatc aattatgttc atagctagaa aagttagtat aaaatcaatt
    12781 ataaattcgc aaagtttata tattgagtat ggtaatttgt ttacatatct gaatatatct
    12841 aaaatagtag tgcatagaaa atcaattgga gtggttatac aaaaatatat taaaaagaaa
    12901 attaatacag ctccaagaga tttatattca aatcctaaga atgataagaa gccaattcct
    12961 ataaccgtga aaatacaaaa agttatacca aaaaaaacag caaaaaataa aaatacagaa
    13021 ataaattcgc ctaaaggaat ccttctttta ttacttatct taatcaactc cttaatttat
    13081 gatatgaaat ttatataaat aataacattc ttaaaaattc ataatagtcc tctgtttcaa
    13141 atagtactgt ttgagaatct atttttttca tatttggata aaaagaggcc ttaaaagctt
    13201 tataatgttc tctaaatttt atttctatct taaatttatc tggcaatttt attaagtgcc
    13261 tagataaatc tccagataaa gcttttttga cacctttttc aatttttttt agtgcaaggt
    13321 ttggatggat tgaaattgaa ccatttccaa ttccttttga cacaggaaca gtgactatat
    13381 ttggatttag tttttttgca ctctcacaaa gcatttcatc tccacttaaa aatgctacag
    13441 gaacattata atatgcagca atataagcat ttatagtaaa ttcagatgct atttcatcat
    13501 ttattttaaa atagtcataa tcaatatcca tagtatgaga aagtggatta ccattacaag
    13561 tagctccaga gtgatagccg gtgaatatac atgcatcaaa gctttcatca attcctgcca
    13621 tcataactag tgggtctttg gtccatcctc taagtatttt gacgttttca ggtaaaatat
    13681 ttggattaat atttctacca ctgtcatgtg catctttaac aagtatttca tgtacaccag
    13741 attgagtagc tccatcacat gctgctgtta tctctttact catttgcgtt ctatagtgtt
    13801 gcgaaaattc acattcaagt tttgtttcat cccaatttac aataccacaa gtaccttcta
    13861 tatcagcact taaatacaat ttcataatat ataccccctt atgtttatta ttgatgataa
    13921 taacagataa cagaaaatac ataatcaata aagggcataa aaatattatg tcttactatt
    13981 aactattatt aaatctgtaa ctgaaaaata actaaattgg tataaaaaaa tataacacta
    14041 tgtgtcattt atttatatag ttttttatat ttgatgttta tataaaattg taacatataa
    14101 gtggttacat ttactagttt aacatagtta aaatagttag gtattatttt taagttaaat
    14161 tgattattat aaaatcgcag aataaaagaa aaatttaatt tattaaaatg catgaaatgt
    14221 tttttcctca acataatgct aaattaaact caaaaagaaa taatagaaaa atcttatgta
    14281 gcttagaaag aatgtgaatg atttaaggga aaaatagaaa tttattatat aatattaggt
    14341 tgtattatat aatacagcct aatattatat gtatatgtga cataaaatct tggtaagtgt
    14401 aaataaaata aatgtggaaa acaagttaat gaaatcaaga aaaaagtaat ttaaaattca
    14461 atttcatctt cccaattaaa ttctcctgat ttttctacac tcttaggcca gtgactacac
    14521 catttaggaa cggacggatt ttcaacccta tcaacgcttg gagtatatgg ctttaagtca
    14581 ataacaggac tattatcatt agcatcaata tatggtattt ttattatgcc attttcatgg
    14641 tcgatattga taatttgtac agcagtcaat gctattggat tgggtcttat aggtgacctt
    14701 gttgcaaata ttcctataac gtctggtcca cttttataag gttttggggt ttctaaaata
    14761 cttcttagtt caggattatc aaacccatca gcccaccaaa atacatttaa atgactgaaa
    14821 tcgtcgagtt catttaatgc aggtacatac tttttgtcta atttaataaa gacctcttcg
    14881 ccttgaatac atattttacc tataggattt attaatattt ctttcataat attacctcca
    14941 actatttaat ttatcatttg taattttagg gtataacatg acatcatgtg agggtcaata
    15001 taaaataata aataaaaata ttaatagtta gaatgataat acatttttta catggagcat
    15061 gacaccagta ataaggcaat cttcgtcaat atcaaaagta gaggtatgca aaggggaagt
    15121 aatgtttttt tcacgatttg cacaaccaag gtggtaaaaa gctccttcac agtgtcttag
    15181 aaaatacgaa aagtcttcag caccaagagt gggcgagtct ttaaggataa tattttcttc
    15241 accaaacaat tctacagcat tttgtttgac gtaatctact agatggtttg aattgataag
    15301 agctggataa ccttcctcaa tttcaacaga aatacttcct ccaaatgttt tacaagtaaa
    15361 atcgcaaatt tctctaattc gtttttttga aaactctcta gtttcaggag tcaaggttct
    15421 caaagtaccc cttataacca ctttgtcaca aactatattt tctttgattc caccttcaat
    15481 ctttcctaga gaaataacca cagaatcagt tggatttgtg tttcgactaa cgattgtctg
    15541 tagagctgta ataatatgag cagatataac aatagcatca ataccttgat gtggataagc
    15601 tccatgacat tttgaaccat gtacagttaa ttgaagtgta ttggtggaag catttagagt
    15661 gttgtatttt aattcaatta gatttttgtt gatattggga tttacatgga gtccaaatat
    15721 atattttaca tttggttttt caagacatcc atcttctatc ataagttgag ccccaccaat
    15781 agtttcctca gcaggttgaa aaaagaattt tacatttaca tttaagtaat cctttaattt
    15841 aaatagtata ttacaagtac ccagtaaaat agcagtgtgt gcatcatgac cacatgcatg
    15901 cattttgcca atattaatag atttataaga ataattaagt tcttctgtta taggtaaggc
    15961 atctatatca gctcttatag caactgtttc aaagtttgga ctcacgttaa tgtatgcagt
    16021 tatccctgta tgattcttat attcaatata atttatacct atttcattta agtatttaat
    16081 aatttttttc ttagtttgaa attcttctaa tccaagttct ggagtcttat gtaaatctct
    16141 tcttatattt ataacccaat catttatctc atgacaaaga gtttttatat taagcataaa
    16201 acacatccta actatattaa aaatttattt ttcttcatta attatattat tttatgtaaa
    16261 tcattattat gtatcaagta caaataaaat atcttataat tatattagag atatttagta
    16321 tggagaagaa tatatcattt tataagaaga ggtgtttaaa tgttaggaat aattgcgcca
    16381 tctgggccat taagaaatac aagtttggag gaaataaagt ttaatttaga aagttatggt
    16441 tacgaagtta agttttctga aagttgtagt ttaaattata aaggttattt agctggtaat
    16501 gatgatatta gagcaaggga tatagaagat atgtttttag ataaagatgt agatataatt
    16561 atgtgcttaa gaggaggtta tggaacaaca agaatattgg ataaaataaa ttatgatata
    16621 ataaagcaaa atccaaaacc atttataggt ttttctgata taacaggttt aaatttagct
    16681 ttctataaaa attgtggtct tttaccatat catggaataa tggcagcaaa tgtaggtaaa
    16741 tggggtaaat ttacctataa atcattggta aatgctttag aatttaaaga tgaattatat
    16801 ttagaaaatc caaaagaaga aaagatttat actgtatgtg aaggtaaagc agagggaata
    16861 ataatgggag gaaatttatc cttaataatt gccacaatgg gaacaaaata tgaaatagat
    16921 gcaaaagata aaattttgtt tattgaggaa attggagaac ctcaatataa actggataga
    16981 atgttaacac aactatattc ttcaggaaaa cttgaggaat gtaatggtat aatttttgga
    17041 gattttaagg attgtattga agaaaatgat ttgatggaat tactaataga atttgcaaat
    17101 aaggtcaata aaccttctat atataatctt cagtcaggac attgtatccc aatgataact
    17161 attcctttgg gcagaatgtg cgaattagat gctacagaaa aaatcgttaa attaaaaaaa
    17221 tagtgtgtat ataaatataa tagaaatatg gtataatctt atacaagtaa aaatttagct
    17281 aggaggatga aaaatgtctg atgatagaat aatagacttt aacgaactaa agaacaaagt
    17341 taaagattct gatgtagata aatttgagca atacatatat aacttatatt tttctgttat
    17401 ggatggaaag atgtctatgg ctgagttttc aagaaaaata ttcgattata tgagagataa
    17461 caacatatct caagaaaaat ttatgaaaat acaaaagcaa tttatggaga gatatggtat
    17521 ggacactgaa gaagtagaaa aacaacttag aaactttgga atcgaccctt ctacagcagg
    17581 ttttatgagt aataatactt ccagcaaagt atctacagaa gatttagagt catttaagaa
    17641 gagtgcagga ttttatgaga aatatggaga aaaaatccaa ccaaagagtt gtataacaac
    17701 atttataaaa aatgacttaa atgatattaa tgtaataatc gaccaagaaa agataatgct
    17761 ttgtagtgat agaaaaataa atcttatgga ttcagaacta aatgagtttt tattagaata
    17821 taagaatatg tttaataaaa aaataaaggt agtaatgtgc gaaactacga ataagtatga
    17881 ttattaaaaa atgaatttaa atactgaagt agtataaatt aattgtacta cttaaaaata
    17941 aaaatcgcaa tttattattc cttataaatt tgtataagga atatctaaac tgcgattttt
    18001 tatattgtga agattagtaa taatagatag tgttttaaat tgtaatcttc tcactaaaac
    18061 tgtgtttttc tattagaaat tttattaaaa ataaatatct gataattgta ttatatggga
    18121 tgtaaagtat tagaagctta acgaatctct tttaatacat taactccatt ttgaatcata
    18181 ctgttgaaaa atactaagtt taaaatctca gcagggtgaa cgttaggaat atcataagta
    18241 tctactaaat tcataggtac aactatattt ttttcaatat tatgttgatt aaaatatgat
    18301 tttagagtta ttgcaaattg atatatacat atatctgtgc aatcgccaac tataattata
    18361 ttatctatat tatcaaaatc caaattttct aatgcaaaaa atccattagt agaattttta
    18421 ggaagaattt gtaggttttc tatcgactta agttcatcaa ccaattcact ttctacatca
    18481 ttttccaaac agtgaacagg ataactcaaa agttctattg atttaggagt atgagaatca
    18541 gtaaatgcta ttactctatt taatttgttt gatatttttt ttgtaaacat ttcaattggc
    18601 tttattaaag actctactct aggagagtat aatgcacctt gtcttgcaaa gccattatta
    18661 acatcaatta taaataaagc agttttactc aaatcataat tttctatttt ttctattggt
    18721 agattatcta aattactctt taaagcttca agttcattta ataagttatc catagttata
    18781 cctccaagat aaatttaata aaattgtaca tattaattat ataaaagtca aggagtacaa
    18841 actattgtat aattagctgt attgttttga gtgtaaaaga tttgaagcaa tatctggata
    18901 gttagtaaaa ataccatcta cattgagttt tagacagtgt agtatatcat cctcttcatt
    18961 tacagtatat acattgacac caagtagatt tttgtgacaa agttcaatta gagattcatt
    19021 tactaaaact agaggtggat gaagataact attacccaga aatttagcaa aattgactac
    19081 attgtttata ggtaaataac acaataatcc tgtttttaca ctagggtcta atttatgaaa
    19141 gttttttaag ctattgctgt tgaaactaga tataagaact ctatttttca tattatactt
    19201 taaaataagc tctaaaacgc ctttttctat attgggatag tttattttgt cagtttttag
    19261 ttcgatattt aaagtaaggt ctgtaggtga gattagaatt aatacttctt ctaaagttgg
    19321 aattttacat tctttattgc tttgatatcc ttcaaaagat gagtttagag tttttaattt
    19381 tcttaaagta aagtctttta caaaaccatg tccattaaaa gttctatcaa ccttttcatc
    19441 atgaataact acaatttttc catccttagt tctgtggaca tcaagttcta ttccatatat
    19501 atccatatcc aaacattttt taaaagctag aatagtattt tctggatact ttccactaaa
    19561 ccccctatga gcataaatat tcatttatat ggttccctcc tataagtata acttatctta
    19621 taaaaattta attaatataa tatatcatat tccaaaaggt attgttatat attagttaaa
    19681 taactatttg taatttatat attatataaa tgtagaaaaa taaaatcata tatgtgttat
    19741 aatatatttt aaacattgta aatattacga atttagtagt cataataagt gtaattttat
    19801 gcaagctcat gtttttagag cttgcatatg tattattaat atatttatat attgtgaaaa
    19861 taaaattcca aaagaatttt ttctatagac ttagtagtaa gggggaattt tgatatgtat
    19921 gaagttaatt tagaacgagc tatgaattat tggatggatt tgttagatga ttatacgaat
    19981 atagcaaaaa ttccaaatga ttttttttat aaagattgtt ctgatgaagt agtagatgag
    20041 gtttttaaga tgaatattga gaaacatgaa aggttaattc aatattctaa agtgaacaat
    20101 attagcatag actctatcat agaaactata tatggcctta tacttcaaaa gtatatatat
    20161 gaagatgatg tagtttttgg taaaacaatg aatataattc caataagagt aagagttaga
    20221 caaagtgaaa aatttataga tgttgtaagg aagctaaaaa ggcagtggag caggtcttta
    20281 gagtatggat actatttgtt atcagatata gagaataaaa atcatctaaa tactaagctt
    20341 ataaacacaa tatttatagc taaaagtttt gatttatcat atgattatga catagaaaaa
    20401 aaaggatatg atttatctgt aatagtatat actaagaggg cacttaaaat aaaaataaag
    20461 tataaaccat ctatatataa aagagaaact attagaagaa ttttaaatca atttgattat
    20521 ataatagagc aaataataga agataatgat attttaatag aagatttaga atttgtatgt
    20581 aaggaagaag aaaaagtatt attggaagac tttaataata atcaatatga agtaccctat
    20641 gataaaagtt atgttgattt atttagggag caagttaagg taacacctag taatatagct
    20701 gctagggatg aaaatagaaa gttaacatat aaagaattgg atgaagtaac tgataaactt
    20761 gcaggttatt taaatcatat aggagtggaa agtgaagaca tagtagctgt aatgttacca
    20821 agagatatta acataattat tacagcaata gggattatga aatcaggggg agcatttttt
    20881 ccaatagaca caagtaatcc agaagaaaga ttgaattacc ttcttgaaga tagtaatgcg
    20941 aaagtagtga taactacaga tgaactcaaa agtaaggttg taaatgagaa taccatagta
    21001 ctcgatataa atgatgaaga aatgtttaag tttggatatg aattaactga aaaaataaca
    21061 tcatcaaatt gtgcatatac gatatctact tcagggtcaa ctggtaggcc aaagaccata
    21121 gctatagaac ataagagttt agtaaatatg tgttattata gcgtaaaatc tataagcgct
    21181 acagaaaatg atatatgtgg tatatactta agtttcagtt ttgatgctgt aatgaaacaa
    21241 ctattccctt atttgcttgt aggtgcatca atagatatta tgcctgaaga agctaaattt
    21301 gatgaatata cagtaaatga atactgtgag gaaaatgaca taactatact agcattgcca
    21361 acagcatttg caaggttatt tatacaaaat tgcaataata attcacttag agttgtacag
    21421 actggaggag aaagattaaa aggatataga aagcgtaatt atgaactata taatgagtat
    21481 ggaccaaccg aatttactgt tgtatcgact tcttttcatg tggacagaga atatggaaaa
    21541 ataccaatag gaaaacctat atttaatact tatgcatatg tattagataa aaagaacaaa
    21601 ctatgtccaa ttggagcacc aggagagcta tgtctatcag gcattcaaat atcaagagga
    21661 tatttaaata aaaaagggct aacagagcaa gtatttgtag agaatcctta caaaacttgt
    21721 gaacataata agttaatgta tagaactggt gatttagtaa gatggttaga tgatggaaat
    21781 ttagattata ttggaaggat ggacaatcaa gtaaaaattg atgagtttag gatagagcta
    21841 tatgagattg aaaatataat aaataatatt acagaaatta aaagtgttgt ttgtatatcg
    21901 agaacaaatg atgatggaga tatttatata tgtgcatatt atgtaattga tgaggaagat
    21961 agtggtaaga taaatgaaag aactattaga gaatatttaa atgagcatct acctccatat
    22021 atgattccaa caataataat gaggatagat aagataccag ttacaccaat cggaaaagta
    22081 aataaaagag cattaccaga ataatttttt tattatgata ggtgtcataa agtgatttca
    22141 gacatttctt tatgacactt atttttataa aataatcaat ttgcataaaa acaagaattt
    22201 tctgtatata tttactatgg tgaaaattta tttataagaa tgattattgt attttttata
    22261 cttttccgtg ttttaaagca ttttttttac aaaattaatt ttacattaaa aaattt
//
LOCUS       DAGSRM010000061        21942 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.061, whole genome shotgun sequence.
ACCESSION   DAGSRM010000061 DAGSRM010000000
VERSION     DAGSRM010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21942
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.061"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            370..681
                     /gene="rpsJ"
                     /locus_tag="MJE33_002443"
     CDS             370..681
                     /gene="rpsJ"
                     /locus_tag="MJE33_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBY9648375.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            773..1402
                     /gene="rplC"
                     /locus_tag="MJE33_002444"
     CDS             773..1402
                     /gene="rplC"
                     /locus_tag="MJE33_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBY9648376.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            1432..2055
                     /gene="rplD"
                     /locus_tag="MJE33_002445"
     CDS             1432..2055
                     /gene="rplD"
                     /locus_tag="MJE33_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBY9648377.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNVIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            2055..2345
                     /gene="rplW"
                     /locus_tag="MJE33_002446"
     CDS             2055..2345
                     /gene="rplW"
                     /locus_tag="MJE33_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBY9648378.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTSSFKKAVVKLTADSKEIEFFQGM"
     gene            2375..3205
                     /gene="rplB"
                     /locus_tag="MJE33_002447"
     CDS             2375..3205
                     /gene="rplB"
                     /locus_tag="MJE33_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBY9648379.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            3240..3521
                     /gene="rpsS"
                     /locus_tag="MJE33_002448"
     CDS             3240..3521
                     /gene="rpsS"
                     /locus_tag="MJE33_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBY9648380.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            3553..3888
                     /gene="rplV"
                     /locus_tag="MJE33_002449"
     CDS             3553..3888
                     /gene="rplV"
                     /locus_tag="MJE33_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBY9648381.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            3911..4726
                     /gene="rpsC"
                     /locus_tag="MJE33_002450"
     CDS             3911..4726
                     /gene="rpsC"
                     /locus_tag="MJE33_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBY9648382.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            4762..5193
                     /gene="rplP"
                     /locus_tag="MJE33_002451"
     CDS             4762..5193
                     /gene="rplP"
                     /locus_tag="MJE33_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBY9648383.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            5195..5398
                     /gene="rpmC"
                     /locus_tag="MJE33_002452"
     CDS             5195..5398
                     /gene="rpmC"
                     /locus_tag="MJE33_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBY9648384.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            5422..5676
                     /gene="rpsQ"
                     /locus_tag="MJE33_002453"
     CDS             5422..5676
                     /gene="rpsQ"
                     /locus_tag="MJE33_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBY9648385.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            5702..6070
                     /gene="rplN"
                     /locus_tag="MJE33_002454"
     CDS             5702..6070
                     /gene="rplN"
                     /locus_tag="MJE33_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBY9648386.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            6094..6399
                     /gene="rplX"
                     /locus_tag="MJE33_002455"
     CDS             6094..6399
                     /gene="rplX"
                     /locus_tag="MJE33_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBY9648387.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            6429..6971
                     /gene="rplE"
                     /locus_tag="MJE33_002456"
     CDS             6429..6971
                     /gene="rplE"
                     /locus_tag="MJE33_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBY9648388.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEMISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            6989..7174
                     /locus_tag="MJE33_002457"
     CDS             6989..7174
                     /locus_tag="MJE33_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBY9648389.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            7207..7605
                     /gene="rpsH"
                     /locus_tag="MJE33_002458"
     CDS             7207..7605
                     /gene="rpsH"
                     /locus_tag="MJE33_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBY9648390.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            7636..8178
                     /gene="rplF"
                     /locus_tag="MJE33_002459"
     CDS             7636..8178
                     /gene="rplF"
                     /locus_tag="MJE33_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBY9648391.1"
                     /translation="MSRIGVKPIIIPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTDNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            8214..8582
                     /gene="rplR"
                     /locus_tag="MJE33_002460"
     CDS             8214..8582
                     /gene="rplR"
                     /locus_tag="MJE33_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBY9648392.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            8603..9112
                     /gene="rpsE"
                     /locus_tag="MJE33_002461"
     CDS             8603..9112
                     /gene="rpsE"
                     /locus_tag="MJE33_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBY9648393.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            9127..9312
                     /gene="rpmD"
                     /locus_tag="MJE33_002462"
     CDS             9127..9312
                     /gene="rpmD"
                     /locus_tag="MJE33_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBY9648394.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            9345..9788
                     /gene="rplO"
                     /locus_tag="MJE33_002463"
     CDS             9345..9788
                     /gene="rplO"
                     /locus_tag="MJE33_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBY9648395.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            9832..11100
                     /gene="secY"
                     /locus_tag="MJE33_002464"
     CDS             9832..11100
                     /gene="secY"
                     /locus_tag="MJE33_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBY9648396.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSGEVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            11117..11767
                     /locus_tag="MJE33_002465"
     CDS             11117..11767
                     /locus_tag="MJE33_002465"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBY9648397.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKN
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVDYYSKQGIIADIKGDQAIDKVFEDIVAALGSGK"
     gene            11768..12514
                     /gene="map"
                     /locus_tag="MJE33_002466"
     CDS             11768..12514
                     /gene="map"
                     /locus_tag="MJE33_002466"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBY9648398.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            12536..12805
                     /locus_tag="MJE33_002467"
     CDS             12536..12805
                     /locus_tag="MJE33_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing protein"
                     /protein_id="HBY9648399.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVKHLKKYNFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            12843..13061
                     /gene="infA"
                     /locus_tag="MJE33_002468"
     CDS             12843..13061
                     /gene="infA"
                     /locus_tag="MJE33_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBY9648400.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            13097..13210
                     /gene="rpmJ"
                     /locus_tag="MJE33_002469"
     CDS             13097..13210
                     /gene="rpmJ"
                     /locus_tag="MJE33_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBY9648401.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            13363..13734
                     /gene="rpsM"
                     /locus_tag="MJE33_002470"
     CDS             13363..13734
                     /gene="rpsM"
                     /locus_tag="MJE33_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBY9648402.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            13770..14168
                     /gene="rpsK"
                     /locus_tag="MJE33_002471"
     CDS             13770..14168
                     /gene="rpsK"
                     /locus_tag="MJE33_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBY9648403.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            14200..14823
                     /gene="rpsD"
                     /locus_tag="MJE33_002472"
     CDS             14200..14823
                     /gene="rpsD"
                     /locus_tag="MJE33_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBY9648404.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            14901..15848
                     /locus_tag="MJE33_002473"
     CDS             14901..15848
                     /locus_tag="MJE33_002473"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBY9648405.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICPPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            15869..16210
                     /gene="rplQ"
                     /locus_tag="MJE33_002474"
     CDS             15869..16210
                     /gene="rplQ"
                     /locus_tag="MJE33_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBY9648406.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            16330..17163
                     /locus_tag="MJE33_002475"
     CDS             16330..17163
                     /locus_tag="MJE33_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBY9648407.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDNLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDDMDTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISSDILTVDEMVMELCQ
                     L"
     gene            17151..18017
                     /locus_tag="MJE33_002476"
     CDS             17151..18017
                     /locus_tag="MJE33_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBY9648408.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDVSFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLDYEGFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKDLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFMGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            18011..18814
                     /locus_tag="MJE33_002477"
     CDS             18011..18814
                     /locus_tag="MJE33_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBY9648409.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMVSIFVVNKFWP
                     YIVVLLCLLAMIKLANIPVKYIVKGVKPLKWIILFTFLINIFFLPGDEIWSFGFLAIT
                     KQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            18846..19577
                     /gene="truA"
                     /locus_tag="MJE33_002478"
     CDS             18846..19577
                     /gene="truA"
                     /locus_tag="MJE33_002478"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBY9648410.1"
                     /translation="MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETS
                     LIGSGRTDSGVHAIGQIANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            19697..20128
                     /gene="rplM"
                     /locus_tag="MJE33_002479"
     CDS             19697..20128
                     /gene="rplM"
                     /locus_tag="MJE33_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBY9648411.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVIVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            20157..20549
                     /gene="rpsI"
                     /locus_tag="MJE33_002480"
     CDS             20157..20549
                     /gene="rpsI"
                     /locus_tag="MJE33_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBY9648412.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
     gene            21008..21760
                     /gene="cwlD"
                     /locus_tag="MJE33_002481"
     CDS             21008..21760
                     /gene="cwlD"
                     /locus_tag="MJE33_002481"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HBY9648413.1"
                     /translation="MEYYSVFISIHIQGDVVRKYIKHIIFSFAMICLVVVSIFEIKNI
                     SEDVIKYMPVTNKTIILDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGL
                     VILTREDDSSLYKEENNKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYG
                     AQTFYPKDKQDSKELSKCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGF
                     LSNEKECKLLTDETYQEKIAWAIYIGIQKYLS"
BASE COUNT         8814 a         2567 c         4495 g         6066 t
ORIGIN      
        1 ctttactaaa ctaatggatt taatataaaa aaagaggggg gtaactcctc tcttttttgt
       61 tttttgttga caagtagcgc taaagcatat atactaaact agtgccttga gacagattaa
      121 caatcaagtt taaggttatg atgttgtaat agaaaataaa attgaaaaaa acagaaaaaa
      181 aacttgaaaa aatgtaacag atgatataaa atataatagt gtgcttgaga gattaagaaa
      241 ataataatta aatcctagtg aaatacgaac tgatatcgat ttaaaagtta caacacaccc
      301 ggggcagtgt atcggtattg gttgtacgca acttgaaaat taaacacgtg ataaaggagg
      361 gaaaacaaaa tggctaagaa tgaaaaaata agaataagat taaaatcata tgatcacaaa
      421 ttattagatt tttcagcagg taaaatagtt gaaactgcta aaaaggctgg atcacaagtt
      481 tcaggacctg tgcctctacc aacagaaaaa caagttgtaa ctatattaag agctgtacat
      541 aagtacaaag actctagaga acaatttgaa ataagaactc ataaaagact aatcgacata
      601 gctaatccaa cacctaagac agttgactca ttaatgagat tagacttacc agcaggtgta
      661 gatatagaaa taaaattata agaaccccta agatattatc ctatagatga ttatcttaga
      721 atgattactc taaaagatgt aatccgctgt aagaattata ggaggtgcta atatgaaagg
      781 aatattaggc aaaaaagttg gtatgactca aatattcact gataaaggtg tagttatacc
      841 tgtaacagct gttgaagcag ggcctatggt cgttactcaa ataaaaacag ttgataaaga
      901 tggatacaat gctatacaaa taggttttga agatgctaaa gaaaaagctt taaacaaacc
      961 taaaaaagga catttagctg ctgctaatgt tttaaagaaa catttaaaag aatttagagt
     1021 agattctgta gaaggataca cagtaggaca agaaataaaa gctgatgtat ttgaagcagg
     1081 tgcaaaaata gatgttactg gaataagtaa aggtaaggga ttccaaggtc caataaaaag
     1141 acatggacaa agtagaggtc ctgaaactca cggttctaga taccacagaa gaccaggttc
     1201 aatgggagct tgttcttacc caggtagagt atttaaaaac aaaaaattag ctggacatat
     1261 gggaagcgta aaggtaacag ttcaaaactt agaggttgtt aaagtagatg ctgacaagaa
     1321 ccttatatta gttaaaggag ctataccagg agctaaaggt tcagtagtaa ctataaaaga
     1381 agctataaag gtttctaaat aatgactaac ctagagaaag gaggaataac aatgccaaaa
     1441 ttaaatgtac taaatgttag tggacaaaac gttggagaaa tagaattatc agattctata
     1501 tttggcgtag aagttaatgg acatgtttta tacgaagttg taaaaaatca attagcaaat
     1561 aagagacaag gaactcaatc tgctaaaact agagcagaag ttagaggtgg cggaagaaaa
     1621 ccttggaaac aaaaaggaac aggtagagca agacaaggtt ctacaagatc tgtacaatgg
     1681 gtaggtggag gagttgcttt tgcacctaaa ccaagaagct acaaatatac attacctaag
     1741 aaggtaagaa gattagctat gaagagtgct ttatcttcaa aagttcaaaa tagtgaagtt
     1801 atagtattag atgcattaaa catggatgct cctaagacta aagaatttgc tcaaatatta
     1861 aacaatataa atgctgctaa aaaagcttta gtagttatag ctgacaaaaa cgataacgta
     1921 ataaaatcag ctagaaatat agaaggtgtt caaactgcat tagttaatac tatgaatgta
     1981 tatgatatat taaaatacga ttcatttata ataactacag atgctgttaa aaaagtggag
     2041 gaggtgtacg cataatgact aatccacatg atgtaataat aagaccagtt gtaactgagc
     2101 acagtatggc tgaaatgggt gaaaaaaaat acacttttgt tgtggctaaa gatgcgaaca
     2161 aaactgaaat aaaaaaagca gtagaaaaag tattcggagt tagtgttgat aaagtaaata
     2221 ctttaaacta cgatggaaaa gttaaaagaa tgggtagaac tcaaggaaga acatcaagct
     2281 ttaagaaagc agtagttaaa ttaactgctg atagtaaaga aatagaattc ttccaaggaa
     2341 tgtagtatta taacaaacga aggagggaaa acagatggct ataaaaaagt ttagaccaac
     2401 ttctcctgcc ttaagacaaa tgacagtttt agtttctgat gaaataacat gtaatcaacc
     2461 agaaaaatct cttttagtta acttaaagaa aaatgctggt agaaacgtac atggtagaat
     2521 aactgttcgt catagaggtg gaggacaaaa aagaaaatat agaataatag attttaaaag
     2581 agataaagat ggtatacctg ctaaggttgc tactatagaa tatgatccaa acagaactgc
     2641 aaatatcgca ttattaaact atgctgatgg agaaaaaaga tatatattag ctccagttgg
     2701 aattaacgtt ggagatacta tattatcagg attaggtgct gatataaaac caggaaactg
     2761 tttagcatta aaagatatgc cagttggtac tatcattcat aatatagaat taaagccagg
     2821 aaaaggagct caattagtta gatctgctgg agtttctgca caattaatgg ctaaagaagg
     2881 aaaaaatgct ttattaagat taccatcagg tgagatgaga ttagtaagca taaactgtaa
     2941 agctactata ggtcaagttg gaaatataga gcatggtaac gtagttatcg gtaaagcagg
     3001 tagaaaaaga catatgggaa taagacctac tgtaagaggt tctgtaatga accctaatga
     3061 ccatccacac ggtggagggg aaggtagatc tccaataggt agaccatctc ctgttactcc
     3121 atggggtaaa ccagctcttg gatacaaaac tagaaagaaa aataaagctt caaataaact
     3181 aatagtatca agaagaacta agtagtaaat ttatttttgc tcggaaagga ggaatataaa
     3241 tgtcaagatc aactaaaaaa gggccttttg tccatgcaag acttttaaag aagatagaag
     3301 ctatgaatgc tagtggaaat aaagaagtta taaaaacttg gtcaagatct tcaactgtat
     3361 ttccacaaat ggtagaaaac actatagctg ttcatgatgg aagaaaacat gttccagtat
     3421 atataacaga agatatggtt ggacataaat taggtgagtt cgttcctact agaactttca
     3481 aaggacataa ggatgacgaa aaatctaata agagaaaata attaaattat gactaagaaa
     3541 ggaggaataa caatggaagc aaaagctact gctaaatacg tacgtgtatc accaagaaaa
     3601 gcaggccaaa tatgtgacct tgttagagga aagaatgttg atgaagcatt agcaatatta
     3661 aagtttactc caagaggagc agcttcaata atagctaagg ttgtaaaatc agctaaagct
     3721 aacgcagaaa acaatcacga aatggatact gaaaaattat atatagcatc aatagttgct
     3781 aatcaaggac caacaatgaa gagattcatg cctagagcta tgggtcgtgc aactacaata
     3841 agaaaaagaa cttctcatat agaggttgtt gttaaagaaa aaaaataatt aatagatagg
     3901 agggaaagta atgggtcaaa aggttaatcc acacggctta agggtcggtg ttataaaaga
     3961 ttgggactca agatggttcg ctactgataa aaaagagttc ggaaacttat tattagaaga
     4021 ccataatata cgtaaattct taaagaaaag attatactca gctggagttg ctaagataga
     4081 aatagaaaga tcagctaaca aaataaaaat ggacttacac gttgctaagc ctggtgtagt
     4141 tataggaaga gctggtgcag gaatcgaagc attaaaagct gaattagaaa aaatgacaaa
     4201 gaaaactata atagttaata tagtagaagt aagaagtaca gataaaaatg ctcaattagt
     4261 agctgaaaat atagctttag ctatagagag aagggttgct ttcagaagag caatgaaaca
     4321 agctatacaa agagcaatga aatctggtgc taaagggata aaagtttctg cttcaggaag
     4381 attaggtgga gctgaaatgg ctagaactga aggttatagt gaaggaaatg tgccactaca
     4441 aactttaaga gcagatatag attatggttt cgctgaagct gatacaactt atggaaaaat
     4501 cggtataaaa gtttggatat gtaatggtga agttttacca actagagacg gtgtaaatcc
     4561 aagagaagaa agcagaaaga gcgatagaag agacaacaag agagataata gaagaaacga
     4621 tagaagagga aatgacagaa gaggaaatga taacagagga aactacagag gacaaagacc
     4681 tcaaggtgga tcaagacctc aaagaactga aaacaaagga aattaataat agttaggttc
     4741 aaggaaggag gaaaaatact catgttaatg ccaaaaagag taaagcgtcg tagagttcat
     4801 agaggaagca tggctgggca agctcataag ggtaataaag ttacttatgg tgagtttgga
     4861 ttagtagcat tagaagcttc ttggataact tctaatcaaa tagaagctgc cagaattgcg
     4921 atgactagat atataaaaag agggggaaag gtttggataa aaatattccc tcataaacca
     4981 gtaacaagaa aaccagcaga gactcgtatg ggtgcaggga aaggttctcc agaatattgg
     5041 gtagctgtag ttaagccagg aagagttatg tttgaattag caggtgtttc tgaagataaa
     5101 gcaagagaag ctatgagact tgctgcacat aaattaccaa tcaaatgtaa atttgttaaa
     5161 aaagaagatt tagaagtaaa gggtggtgaa taggatgaaa gctaaagaat taagagattt
     5221 aacaagcgaa gagctaatga acaaattaaa cgacttcaaa agtgaattat ttagcttaag
     5281 attccaatta gctactggtc aattagaaaa tacagctaga ataaagtttg ttaagaagga
     5341 tatagcaaaa gttaaaactg ttcttgctga aagaaagtta tacgaaacta gagcttaatt
     5401 tggaaaggag gctttttaaa catggaaaga ggaagaagaa aagttagaat aggccgtgtt
     5461 gttagtgata agatggataa aacaatagta gtagctgttg aagaattcgt acgtcatcca
     5521 ctatataata aacgtgttaa gagaactaag aaattcaagg ctcatgatga aaagaatata
     5581 tgtaacatcg gagatagagt taaaataatg gaaactagac cattatctaa agataagaga
     5641 ttcagactag ttgaagttgt tgagaaagtt aagtagtttt tgaagaagga gggattacga
     5701 tatgatacaa caagaatcac gtctaagagt tgctgataac tcaggagcta aagaactttt
     5761 atgtatacgt gttctaggcg gaagtaaaag aagatatggt aacataggcg acgttatagt
     5821 tgctactgtt aaaagtgcaa cacctggtgg agttgtaaaa aaaggtaagg ttgttaaagc
     5881 tgttatagta agaagcaaac aaggcgtaag acgtaatgac ggaagctata tatcttttga
     5941 tgaaaatgct gctgttataa taaaggacga taaaactcca gtaggaactc gtatattcgg
     6001 gcctgttgct agagagttaa gagacaatga attcatgaaa atagtttctc ttgctccaga
     6061 agtactataa ttaaggaggt gcaataggac atgatgcgtg ttaaaaaagg tgacactgtt
     6121 gtagttatag caggtaaaga taaaggtaaa aaaggttcag ttttaaaggt atatcctaaa
     6181 actagcaaag tgttagttga aggtgtaaat gtaataacta aacatcaaaa accaagtgct
     6241 atgaaccaac aaggtggaat aataaataaa gaagccccaa tacacatatc taatgtaatg
     6301 ccatttgatc ctgaaacagg aaaaggcgtt agagtaagat atgaagtaaa agatgggaac
     6361 aaagtaagag tatcagcaaa gagcggaaaa gaattataat aaatattgct tgaaaggagg
     6421 gaccttaaat ggcttctaga ttacaagaaa aatacatgaa agaagttgct cctgctttaa
     6481 tggagaaatt tggatacaaa aacgtaatgg agatacctaa gttaaataaa atagttatta
     6541 acatgggtat aggtgacgct agagaaaatc caaaaggatt agaaaaaggc gttgaagaat
     6601 tagaaatgat atcaggacaa aagcctgtta taactaaagc tagaaaatct gttgctaact
     6661 tcaaattaag agaaggaatg ccaataggaa caaaagttac attaagagct gacaaaatgt
     6721 tctactttat ggacaaatta gtttcagttt ctttaccaag agttagagac tttagaggag
     6781 ttaaccctaa tgcttttgat ggtagaggaa attatgcttt aggagttaaa gaacaattaa
     6841 tattccctga aatagaatat gataaaatag ataaagtaag aggaatggat ataatatttg
     6901 ttactacagc aaaaactgac gaagaagctc gtgaattatt aaaattatta ggaatgccat
     6961 tttctaagta agcaaaggag ggattcccgt ggctagaaaa gcgatggttg ttaagcaaca
     7021 aagaaagcaa aagtacgcta ctagagaata cactagatgt actatatgcg gaagaccgca
     7081 ttcagttttg aaaaaatttg gtatatgccg tatatgcttt agagaattag cttataaagg
     7141 tcaaatacct ggtgttagaa aagcaagctg gtagaagctt gattagaatg gaaggagggc
     7201 aacaaaatga caatgacaga tccaattgca gatatgctta ctcgtataag aaatgctaat
     7261 gttgttaagc atgaaactgt agatgttcca gcttctaata tgaagaaaga attagctaga
     7321 atcttattag aagaaggttt cataagaggt tatgatgtta tagaagatgg aaaacaaggt
     7381 atcataagaa tacaattaaa gtacggacaa gaaggcgaaa gagttataac aggtcttaag
     7441 aaaatatcta agcctggaat gagagtttac gctgctaacc atgagatacc aaaagtatta
     7501 aacggattag gaatatcagt tatatctact tcaaaaggaa tattaactga caagcaagct
     7561 agaaaagaaa atgttggtgg agaagtaatc tgctacgttt ggtaattaat atgaacaatg
     7621 taaggaggtg cgaccatgtc aagaataggt gttaagccaa taataatacc agcaggtgtt
     7681 gaagttacta tagctgaagg aaatttagtt acagtaaaag gtccaaaagg aactttaact
     7741 aaacaattaa gtgctgagtt aaacataaaa aaagaagaaa atacaataat ggttgaaaga
     7801 ccaactgata ataaaaaaca tagatcttta cacggactaa ctagaacttt attagataat
     7861 atggttgtag gtgtaaatac tggtttcgaa aagaaattag aattaaaagg tgttggatat
     7921 agagctcaaa aacaaggaaa gaaattagtt atgaacttag gtttctctca tccagttgag
     7981 atggaagatc cagaaggaat aactgttgaa gctccaaacc aaacagagtt aatagtaaaa
     8041 ggtatagaca agcaattagt agggaattat gctgctaaga taagagcttg gagaaagcca
     8101 gagccataca aaggtaaagg tataaaatac gttgatgaag taatcagacg taaagaagga
     8161 aaaactggta aaaaataata ccaaaactta aacgctagaa aggagtgagc taggtgttaa
     8221 aaaaggctga taaaaatgct aatagacttc aaagacataa gagagttcgt agaaaaataa
     8281 gcggtacttc tcaaagacca agattatgtg tatttagaag tgctaacaat atatatgctc
     8341 aaataataga tgacacaaaa agagtaactc tagttgctgc atcttcttta gaagcagaag
     8401 ttaagagtgc tgtcaatcat acaggaaaca aggaagcagc taagaaagtt ggagaattag
     8461 ttgctaaaaa agctgttgaa aaaggaatca ctgaagttgt atttgacaga ggtggatact
     8521 tatatcatgg aagaattcaa gaattagctg aaggtgctag agaagctggt cttaagttct
     8581 aatacgaagg agggaaaaat acatgctacg tcgtaagccg atagatgcag gacaacttga
     8641 cttacaagaa aaagttgttg aagtaagacg tgttactaag gttgtaaaag gtggtagaaa
     8701 tttcagattt gcagcattag tagttgttgg agatgaaaac ggacacgttg gtataggtgc
     8761 tgggaaagca atggaagtac cagacgctat aaaaaaagca gttgaagatg ctaagaaaaa
     8821 tctaatagta gtgccaatag ttgggactac aattcctcac gaagtacgcg gacactttgg
     8881 tgctggaaat atattaataa tgcctgctgt agaaggtaca ggagtaatag ctgggggacc
     8941 tgctagagcc gtacttgagc tagctggact aaaagacgtt agagctaaat ctttagggtc
     9001 taacaatcca agaaatatgg taaatgctac aatagaagga cttaattctc taaaaacagt
     9061 tgaagatata gctaaactta gaggtaaaaa agttgaagaa cttctagggt aaggaggtag
     9121 tgtaaaatgg ctaaattaca aataaagtta gttagaagtg ttataggaac tactcctaac
     9181 caaaaaaaga atgttgaagc gttaggatta agaaaaagag aacaagttgt tgtaaaagaa
     9241 gacaatgctc aaacaagagg tatgataaat aaagtaagtc acttattaga agtaactgaa
     9301 atagctgaat aatttttata aagtagtgca aggaggtgca atccatgaag ttacatgagt
     9361 taaaacctgc tgaaggtgca gtaagagcta agagaagatt aggtagaggt actgcaactg
     9421 gacaaggtaa aactgcagga cgtggacaaa aaggtcaatg gtctcgttct ggtggtggag
     9481 taagagtagg atttgaaggt ggacaaatgc cactagctag aagacttcct aagagaggtt
     9541 tcaataacat atttaagaaa gtttacacag aagttaatgt tgaagtttta aacagatttg
     9601 aaaatggaac agaaataact gcagaattat taaaatctac taaaactata agcaaaatag
     9661 gtaaagacgg aattaaaatc ttaggtgaag gtaatttaga gaaagcttta actgttaaag
     9721 ctgctaaatt tacagcttca gctcaagaaa aaatagaaaa agctggtgga aaagcagagt
     9781 tagtataata acctgctaat ctcctagcca gtacaggcgg ggtgaattag cgtgctgtca
     9841 aaattaaaac aagcttggaa gattaaagca gtaagaaaaa aagtaatgta tacacttatg
     9901 atgatcgtga tattcagaat aggtactact ataccagttc caggcataga cactagtatt
     9961 atacaaaaga tggtaggcgg caatagtcta ctttctctgt ataatatgtt tactggtgga
    10021 gcatttagta acttttcact atttgcttta ggtataagtc catatatcac tgcatcaatc
    10081 ataatacaac ttctaacagt aggttttgaa agtttagcag aacttcaaaa atctggtgaa
    10141 gaaggaaaga aaaaaatcaa taagtacact aaatatacag cactagctct agcagttgta
    10201 caagctttgg gaattacatt aggaattgta agaagtgctt taatatcaaa tagtgtgttt
    10261 tttatcacaa ctgttgtaat aacattagtt tcagcaagta tgttagtaat gtggattggg
    10321 gacaaaatta ctgaaaaagg aatcggtaat ggtagttcag taataatatt tgcaggtata
    10381 atatcaagaa tacctacaga tgttattaaa atatcgcaac aagttaaatc tggcgaagtt
    10441 gcaccttggg ttatagtgat tctagctgta gttatattac ttacagtaac aggagttaca
    10501 tttatacaag aagcaactag aaagatacct gtacaatatg ctaaaagagt tgttggaaga
    10561 aaaatgtatg gaggacaaag ttctcatata ccaatgaaag ttaaccagtc aggagttatg
    10621 cctataattt ttgctagttc acttttagct ttccctcaaa caatagctat gtttatgggt
    10681 ccaaatgctc aggcttttgt tcaaaaatac ttaagtatgg caacagaaca aggattttgg
    10741 acatatagat caattgagat tctacttata attttcttct catattttta tacaactgta
    10801 tcatttaata cagaagacat atctaaaaat atgaaaaata atggtggatt tataccaggt
    10861 ataagaccag gtgagcctac tatggattat ttaaatagaa ttttaactag attaacttta
    10921 gctggagcta ctttcttagc aataattgct atggtgccag ctcttacaac acattatatg
    10981 aaagtaaata tgagtctagc tgggacttca ttgctaatcg ttgttggagt tgctttggag
    11041 cttaagagac agttagagtc aaacttagtt atgagaagct atcaaggttt cttaaaataa
    11101 aatggagatg gataatatga gaataatatt acttggacct ccaggtgctg gtaaaggtac
    11161 tcaggcggca ggaatagtag aaaaatacaa tatacctcat atatcaacag gagatatatt
    11221 cagaaagaat ataaaagagg gaacagaact tggaaaaaaa gctaaagaat acatggacca
    11281 aggtttatta gtaccagatg agttaactgt aggtttagtt actgatagaa tatctcaaga
    11341 agattgtaaa aatggattta tgttagatgg atttccaaga aatgtagcac aaggagaaca
    11401 tttagatatc ttcttaaaaa atgctggtat atcactagat aaagttgtca atattgaagt
    11461 tgataagagt atattagtgt ctagagcagt tggtagaaga atatgtaagt cttgtggagc
    11521 tacttaccat gttgagttta atcctcctaa agtagaaggt gtatgtgatg tatgccaagg
    11581 agaattatat caaagagctg atgataatga agaaactgta tctaagagaa tacaagttta
    11641 tctagatgaa actaagcctt tagtagatta ttatagcaaa caaggtataa tagcagatat
    11701 aaaaggtgat caagcaatag ataaagtatt tgaagatatt gtcgcagcct taggaagtgg
    11761 aaaataaatg attatcttga aatcaaaaaa agaaatagaa cttttaagag aagcaggtaa
    11821 aatagttgct gacactcatg aagttttaag aaaagctatt tctccgggaa tatctacttt
    11881 agaattagat aaaatagctg aagagaatat aagaaaatac aatgcagaac cttcttttaa
    11941 aggatatgga ggttttccag gaagtatatg tgcttctatc aatagagagg tagtacatgg
    12001 tattccagga gaaactatat tacaagaagg tgacattgta agtttggata taggagccta
    12061 ttataaagga tatcatggtg attctgcgaa aactcacgga gtaggcatga tatctgaaga
    12121 agataggaaa ttgatagaag taactagaga aagcttctat gaaggaataa aatttgctaa
    12181 attaggatat agactttcag atatctcaca cgcagtacaa acacacgttg agaaacatgg
    12241 tttctcggta gttagagact tagtgggtca tggagttgga gcaaatcttc atgaagaccc
    12301 acaagtacct aattatggac tcccaggaaa aggaccaaga cttagagaag gaatggttat
    12361 tgctatagag ccaatggtta atgcaggtcg ttatcatgta aagactctat cagatgggtg
    12421 gacgactgtt acaatagatg gaaaaaaatc agctcattat gagcatacga tagcaattac
    12481 tgaacatgaa cctttgatat tgacaaagtt gtaatttaaa aagtaggtga aaaaagtgct
    12541 atcagataat ttaagtatag gtcaagttgt taaagtttca ttaggacgag ataaaggaaa
    12601 tttattcttt gtagtcaaaa taattaataa tgaatatgta ttaatagctg atggaaaaaa
    12661 gagaaaactt gacaaaccta agttaaagaa agtgaagcac ttaaaaaaat ataattttat
    12721 taatgatgaa gttagaaaaa gagtagtatc tggacaagag attacagatt cttttttaag
    12781 agctgaactt actaagttaa attagataat ttaacttatt gaaatggagg gctggttagt
    12841 taatggccaa aaaagatgtt atagaattag aaggtacagt ttcagaagct ttacctaatg
    12901 ctatgttcaa agttaaacta gaaaatggac atgagatatt atgccacatt tctggaaagc
    12961 taagaatgaa cttcataaga attcttgaag gtgacaaggt gaatgttgaa ctttctccat
    13021 atgaccttac aagaggaaga attacttggc gtaagaagta gactatatta atatttagtc
    13081 taaggaggga ttaataatga aggttagacc atcagtaaaa ccaatatgtg aaaaatgtaa
    13141 agtaataaaa agaaaaggaa aagtaatggt tatctgtgaa aatccaaagc ataagcaaaa
    13201 gcaaggataa gaaattatta ctagcatttt gttaaatttt atagtataat actatattgt
    13261 gatgttaaca aaagaacgtt aaaatcgttg aacaatctgg cgtaggattg gcatatgatg
    13321 tgaagtttta aacactaaaa actatgtgta ggaggtgcga tcatggcaag aatagctggg
    13381 gtagatttac ctagagaaaa aagagcggag ataggcttaa cttatatata tggtataggt
    13441 aaagcaactg ctaacgaaat attagctaag gctgagataa atccagatac tagaataaaa
    13501 gatttatcag aagatcaagt taatgagtta agaaaagtta tagatgacga tttcttagtt
    13561 gaaggtgact taagaagaga aattgcttta aacataaaga gattaagaga tataaaatgt
    13621 tatagaggaa taagacacgc taaaggtctt ccattaagag gacaaagaac taagactaat
    13681 gctagaacta gaaaaggtcc tagaaaaaca gtatctcgta aaaagaaaaa ataaataaat
    13741 aataactaga tgaaggaggg aaaaggaaaa tggctaaacc aaaaaagaaa gttacacgta
    13801 ttagaagaag agaacgtaaa aatatagaac gtggtcatgc tcatatacaa tcaactttca
    13861 ataatacaat aataacttta actgacgttc acggaaatgc tatatcttgg gcaagttctg
    13921 gacaattagg attcaaagga tcaagaaaat caactccatt tgcatctcaa atggctgctg
    13981 aaacagctgc aaaagctgcg atggaacacg gactaaaaag tgttgaggta ttcgtaaagg
    14041 ggccaggttc aggaagagaa gctgcaataa gagctttaca agcaactgga ctagaagtaa
    14101 ctatgataaa agacgttact ccaatcccac ataacggatg cagaccacca aagagaagaa
    14161 gagtgtaatt aataagagta caataggagg tgtagataaa tggcaagata tacaggtgca
    14221 tcatgtagac aatgccgtag agagggaatg aaattattcc ttaaaggtga tagatgttat
    14281 acagacaaat gtgctatagt aaaaagaaac tatgctccag gtcaacatgg ccaaggaaga
    14341 aagaaagttt ctaactatgg attacaatta agagaaaaac aaaaagttaa aagaatatat
    14401 ggagttttag agactcaatt tagaaattta tatgaacgtg ctgaaaatat gcctggtaaa
    14461 gcaggtgaga acttattaag tttattagag agaagattag ataatgtagt ttacagaatg
    14521 ggactagcat cttctagaaa agaagctaga caattagtga ctcatggtca tttcacttta
    14581 aatggaaata aagtagacat accttcatta atagttaaag ttggagatgt tatagaagtt
    14641 aaagagaaat caagatcttc tgcaaaattc aaaaatttag tagaagttaa ctcaagaata
    14701 gctcctaagt ggttagaagc taatgtagaa ggaatgacag caaaagtagt tggtgttcca
    14761 acaagagaag atatagatct tgagatagca gagcacttaa tcatagaact ttactctaaa
    14821 taataagcac acttttaaaa attattttaa aattttgttt accctcagtg gattactgaa
    14881 ttttaaggag ggttttgtcc atgatagaaa tagaaaagcc aaaagtagat atagttgagc
    14941 ttagtgaaga ctatagatat ggtaagtttg ttatagaacc tctagaaaga ggatatggaa
    15001 taactatagg taacgcatta agaagaatat tattatcatc attaccaggt gtagcagtga
    15061 atgctataaa aatagatgga gttcttcatg aattttcaac aatacctggt gttaaagaag
    15121 atgttactga gataatatta actttaaaag agctttcagc aactatagat ggtgaaggaa
    15181 gtagaacact taaaatagaa gctcaaggac catgctctat cacaggtgca gatataatct
    15241 gtcctccaga tgttgaaata ttaagtaaag atttagcaat agctacatta gatgataatg
    15301 ctaaacttaa tatggagata tttgtagata aaggtagagg ttatgtttct gctgaagaaa
    15361 ataaaacaga gaatgttcca ataggtgttt tacctgtgga ttcaatatat actcctgttg
    15421 agaaggttag ttatcatgtg gaaaacacaa gagttggtca aaaaacagat tatgataaat
    15481 tagtactaga agtttggact aatggtagta taaatcctca agagggaata tcattagctg
    15541 caaaggtatt agttgagcat ttaaacttat ttatagactt aactgaacat gtaagtagtg
    15601 ttgaaatcat ggtagaaaaa gaagaagatc aaaaagaaaa agttcttgaa atgactatag
    15661 aagaattaga tttatcagtt agatcatata actgtttaaa gagagcggga attaatacag
    15721 ttgaagaatt agctaataaa tctgaggatg atatgatgaa ggttagaaac ctaggtaaga
    15781 agtcattaga agaggtaata caaaaactag aagaacttgg attgggatta aaaccaagtg
    15841 aagaatagcg agcaaaggag ggataagaat ggctaagtac cgtaaattag gacgtgaaac
    15901 agctcacaga aaccttatgt taagaaactt agtaacttgc ttactaagaa gtggaagaat
    15961 agaaactaca gtgactagag cgaaagaaac tcgtagaatg gctgaaaaga tgataactct
    16021 tgctaagaga ggagatcttc atgctagaag acaagtttta gcttatgtta tggatgaaac
    16081 agtagttaat aacttattca cagatttagc tccaaaatat gctgagagaa atggtggata
    16141 cactagaata ataaaaatag ggccaagaaa aggcgatgct gctgaaatgg cttttataga
    16201 attagtatag taaggaaagg attaggcttg atggcttaat ctttttttta tttttttgac
    16261 taaggtatga tataataatg tgtaaaatga tttttataag ttattgctta ctaaaaggag
    16321 tatgagttaa tggataatat agtaaaggta aataatattt cgtttgagta tattacagat
    16381 gaagcaaaac ttaaagcaat agataattta agcctagatg ttaaaaaagg agaatttgtt
    16441 gcgataatag gacataatgg ttctggtaag tctactttat ctaaaaactt aaatgctatt
    16501 cttatgccca ctgaaggaaa tattcttatt gatgatatgg atactaaaga agaagaaaga
    16561 ttatgggata taagacaaac tgcaggcatg gtatttcaaa atccagataa tcaaatagtt
    16621 gctactatag tagaagaaga cgttgcattt ggacctgaga acttaggtat agaaccaaaa
    16681 gaaataagac gaattgtaga ggagtcttta aaaagtgtag gtatgtatga tttaagagat
    16741 agacagcctc atttgttatc aggaggtcaa aagcaaagag ttgcaatagc aggaattatt
    16801 gctatgaggc ctaaatgtat aatctttgat gaagcaacag caatgttaga tccatctgga
    16861 agaaaagaag ttatgaaaac aataaaaaga cttaataagg aagaaaatat aactgttata
    16921 catataacgc actttatgga agaagcagta gaagctgata gagttgtagt tatggaaaaa
    16981 ggaaagaaaa tcttagaggg aactcctaga gaagtattca gtaaaataaa gatgttaaag
    17041 gaaatcggtt tagatgtacc ttgtatgaca gagttatcta gtttattaat agaagaaggt
    17101 ataaatatta gtagtgatat attaactgtg gatgagatgg tgatggaatt atgtcaatta
    17161 tagtaaagaa tttaacacat atatataatg aagggatgcc ttttgcaagt aaggcacttg
    17221 atgatgtttc ttttgagatt aaagatagag attttgttgg tcttataggt catacaggct
    17281 caggaaaatc tacattaata caacatttaa atggattact aaagccttcc tcaggagaaa
    17341 tatttataaa tgattttaat ataacagata aaaacttgaa tttaactgag attagaaaaa
    17401 gagtaggggt tgtatttcaa tatccagaat accaattgtt tgaagaaacg attgataaag
    17461 acatagcttt tggaccctct aatttaggct tagaggagtc tgagatacat aacagggtaa
    17521 aagcatcaat ggaagctgta ggacttgatt atgagggatt taaagacaaa tctccttttg
    17581 aattatctgg gggacaaaag cgtagagtag caattgctgg agttatagcc atgaacccag
    17641 aagttctcat attggatgaa ccaactgctg gtcttgaccc tggtggaaga gatgaaattt
    17701 ttaacttaat aaaagactta catgaaaaaa agaatatgac tataatacta tcatcacata
    17761 gtatggatga tatggcaaag ttagcaaaaa ctttaattgt tatgaatcat ggtagcgtag
    17821 aatttatggg aactcctaga gaagtattta aatcaaatgc tagtaaatta aaagatatag
    17881 gattagatat tccacaagtt ttagagcttg cattgaaatt gagagaaaaa ggctttgaca
    17941 tcagtgagga tatattgact ttagaagaag caaaacagga aatattaaaa gttgtgagag
    18001 gacgaggatt atgttaaaag atataactat agggcaatac tacccaacaa gttctgctat
    18061 tcataaatta gatccaagga taaagcttgt agcgacaatt gtatttatgg tatctatatt
    18121 tgtagtaaat aaattttggc cttatatagt agttttatta tgtttattag ctatgataaa
    18181 attggctaat ataccagtca aatatattgt taagggtgtt aaacctctta aatggattat
    18241 tttgtttaca tttcttataa atattttttt cttaccaggt gatgaaatat ggtcatttgg
    18301 atttttagct attacaaaac agggtctaag acaagcaatt tttatggcaa taagattaat
    18361 atttttagta gttggtacat cattgcttac tttaacaact tcaccaatag aattaactga
    18421 tggaatagag agattgttga atccatttaa gaaaattggt ttgccagttc atgaacttgc
    18481 aatgatgatg acaattgctt tacgttttat tcctacattg ttagatgaga cagacaaaat
    18541 aatgaaggct cagatgtcta gaggtgcaga ttttgaaagt aagaatctta taaatagagc
    18601 aaagaattta gtaccacttt tggtaccttt atttgtaagt gcatttagaa gagctgatga
    18661 attggctatg gccatggagg ccagatgtta cagaggtgga cataatagga ctaagatgag
    18721 agagtctgtt atatcaagaa gggattatat ggcatgtgta tttcaggttg tttatctggg
    18781 agcaattata gctactagat ttatagcaat ttaattaata agttatagat ataatgaggt
    18841 aaagtatgag aaatataaaa attaaaatcc aatacaatgg taagaattat tgtggatggc
    18901 aaaagcaacc tgattcttta gggatacaag gtacaattga aagggctata tatgatataa
    18961 caaaagaaga aaccagtctt atagggtctg gtagaactga ttcaggagtt catgctattg
    19021 gtcagattgc aaattttaaa attaattcag gtataagtat agaaagtata ccaatggcac
    19081 taaatgccaa gttgccaaaa gacatatcag ttatagaagc atgtgaagta aatgatgatt
    19141 ttcactccag atacagtgca aaaggtaaaa catataaata tttagtatat aatagcaagt
    19201 ttagaaatcc aatacttagt gagatatcat atcaagtaaa atatgaactt gattttgata
    19261 aaatgtgttc agaagcaaaa agtttattag gaacacatga ttttaaaggt tttatgagtt
    19321 ctggttcatc tgtaaaagat acagttagaa cgatatatga tattgatata agtaaaaaag
    19381 atgacttgat tacttttgag attagtggta atggtttttt atataacatg gtgaggatta
    19441 ttgttggaac acttgttgat atgggtcgtg gcagaattaa cgaaccattt ttagatataa
    19501 tacaatcaaa aactaggtca agatgtggac atacagctcc agcacaagga ttatttctca
    19561 aaaaagttca ttattaactt gacacaccag gctacatgta ttaaaatata tgagaatgtg
    19621 ttaataagtc cacttgcccc ggacttgaca taaacggtaa aaaaatttta tgaaaagtag
    19681 ttaaggaggg acagttatga aaagttatat agctaagcca gctgatgtac aaagaaaatg
    19741 gtacttagtt gatgctgaag gaaaaacatt aggtcgttta gcaacagaaa ttgcgacagt
    19801 attgagaggt aaacataaac caacatttac tcctcacgtt gatggtggag attttgttat
    19861 tgtagtaaat gcagaaaaaa tagttttaag tggtaagaaa ttagatcaaa aatattacag
    19921 ataccatact ggttatgtag gtggattaaa agaaatatct tacagagata tgatggataa
    19981 gaaaccagaa gaagttatat cacatgctgt aagcggtatg ttacctaaaa ataaattaag
    20041 aagcagaatg atgactagat taagagtatt tgcaggtgct gagcacactc atgcagctca
    20101 aaatccagaa gttttaaact ttaaataaga ttaataggtg aaaggaggag tttataatgg
    20161 ctaacgttca atattatgga actggaagaa gaaaaagttc tgttgctaga gtaagactag
    20221 ttgcaggtga aggaaatata ttagtaaatg gaagagcatt agaaaattat tttaattatg
    20281 agacattaat aagagatgtt aaacaaccat tagttttaac tggtaatgaa aataaatatg
    20341 atgttatagt aaaagttgaa ggtggaggat tcactggaca agctggagct ataagacatg
    20401 gaatatctag agctttatta aaagctgatt tagatttaag acctgcttta aagaaagaag
    20461 gtttcttaac tagagatgca agaatgaagg aaagaaagaa atacggatta aaagcagcaa
    20521 gaagagctcc acaattctca aaaagataat tacttatttt tttggaaaag caaaatcctt
    20581 tatttactgt tattcagaaa gtattggtat tttaaaaatg ttgggttaag ttaattattg
    20641 atatataata aaataataag atatcttatt aggatgtttt tcattaagaa gaacatcctt
    20701 tttattttga ctatttaata aaaaacatat gctaataaca tttaaatttt atttttataa
    20761 tattgtaatt taaaagttta tataaggtta tatattttaa gatattattt aacaatataa
    20821 attctgaata tacgttatca aagtttataa tataaactaa gtataattta ttgtatagta
    20881 ttgaatgtta gataaacata gtgaattaaa taggggcaga aatataatat cattacataa
    20941 taatatatta ataccaataa ataaagatat atcaatatta aaaaaattta aatgtttgta
    21001 ctaatatatg gaatattata gtgtttttat aagtatacat atacagggtg atgttgtgag
    21061 aaagtacata aaacatataa tttttagctt tgcaatgata tgtctagtag tagtatccat
    21121 atttgaaatt aaaaatattt ctgaagatgt tatcaagtat atgccagtaa caaacaaaac
    21181 tataatttta gatgctggtc atggaggcat tgatccaggt gcattaaata aggataagag
    21241 tacatctgaa aaggatatta acctagcaat aacacttaag cttagagagc ttatagaatc
    21301 aagtggtggc cttgtaatat taactcgaga agatgacagt agcctttata aagaagaaaa
    21361 taataaaact acaagacaaa aatataatga gaatttgaaa aatagaaaag aaataatatc
    21421 taattctaat gccaatatgt ttgtctctat acatcttaat gcatttgaac aatcaaaata
    21481 ctatggagct caaacttttt atcctaaaga caaacaagac agtaaagaat tatcgaagtg
    21541 tattcaagaa gaacttaaaa gagtagtaga taagacaaac aacagagaag tcaaaccaag
    21601 ggatgatata tatcttttga aagaaaataa tattccatca gtactgatag aatgtggttt
    21661 tttatcaaat gaaaaagagt gtaaactctt aactgatgaa acatatcaag aaaaaatagc
    21721 atgggcaatc tacataggaa tacaaaaata tttaagttga aataactata aaaataggtc
    21781 taaatatgta aatagcttgt attaaagcag ttaaaatgca ttaatatagg ctatttttat
    21841 tttgacaaaa aaatatttaa aataaaagtt aaaaagttgt tgacttagaa taatatagat
    21901 gatattatat atgagtgccc aaaaggagca ccaaaataag ac
//
LOCUS       DAGSRM010000062        21934 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.062, whole genome shotgun sequence.
ACCESSION   DAGSRM010000062 DAGSRM010000000
VERSION     DAGSRM010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21934
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.062"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            <1..479
                     /locus_tag="MJE33_002482"
     CDS             <1..479
                     /locus_tag="MJE33_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBY9648414.1"
                     /translation="KLQRELSRKTIGSLNRNKARLKVARLQEHIANQRNDFLQKLSTK
                     LIKENDIICIEDLQVKNMIRNRKLSRSISDVSWSEFIRQLEYKANWHGRQIVKVGKFF
                     ASSQICNKCGYKNEEVKDLNIREWIYPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            911..1165
                     /locus_tag="MJE33_002483"
     CDS             911..1165
                     /locus_tag="MJE33_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648415.1"
                     /translation="MENKDIKLTICIFAFVIGIIVLFCCIGLGQFNMDCIIKQNGGSI
                     ATEQYNIYLEQSISQYRNFGSILVLLGGIGILFDKSVRKY"
     gene            1201..1524
                     /locus_tag="MJE33_002484"
     CDS             1201..1524
                     /locus_tag="MJE33_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648416.1"
                     /translation="MARDVFSVKMTPLEAQKMVEKNLEADLVSVDIYNLENNKSIIVT
                     IFEKYYARSNSDAGLVVICENTTGKTLVKITSTGSAIGLFQIDWGTGNNLIKRVKKIL
                     IDYII"
     gene            1798..1950
                     /locus_tag="MJE33_002485"
     CDS             1798..1950
                     /locus_tag="MJE33_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648417.1"
                     /translation="MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINF
                     KYHRKK"
     gene            2144..3067
                     /locus_tag="MJE33_002486"
     CDS             2144..3067
                     /locus_tag="MJE33_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HBY9648418.1"
                     /translation="MVGAFVLPNYAIILSEMSNLWGNFIMWILVPIITIVLLIIAVSS
                     MQYILVMIAFLLIIYSFIEKKIVMGLVSVLFFTYSIYLCATWEDKSLIADNKVETVRA
                     QRETVEREKEMERRRIQEEVDKERYIEKHGMEISENDLKVKLEALVPQEYKGKKYELK
                     VGKFKRYSMYFDLTVQNEKFSNSEECKKFVKEIANDLKKIKISKAYFKFHSKDDGGIY
                     NSVYIDYFRNIQNNVDNVENLEFNEFELKTEEEEKREQEKIEQEKNSYNNYIQNRVVD
                     PLDRIKKLKELLDSGAITQEEYNKKKKELLE"
     repeat_region   3334..4413
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3334..3362
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            4604..4756
                     /locus_tag="MJE33_002487"
     CDS             4604..4756
                     /locus_tag="MJE33_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648419.1"
                     /translation="MKNRRKDKRGFSVLLDKEKLDKFDEVLEEKNLTKKEWLEEKIDE
                     ELEQKE"
     gene            4877..5690
                     /locus_tag="MJE33_002488"
                     /pseudo
     CDS             4877..5690
                     /locus_tag="MJE33_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888845.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin Bro"
     gene            5742..5903
                     /locus_tag="MJE33_002489"
     CDS             5742..5903
                     /locus_tag="MJE33_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648420.1"
                     /translation="MCENLLDNMYIEKRKEEYRIKLLKIRETDIDIYNKLESIVYKLS
                     EKKLEKNNK"
     gene            complement(6232..6696)
                     /locus_tag="MJE33_002490"
     CDS             complement(6232..6696)
                     /locus_tag="MJE33_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648421.1"
                     /translation="MDFGEVKSELSDKDLKDNEEALNKYKKYYLKVYKYYSEMDSIKS
                     IQDIEIEQSRLKGQLQKNSNPIFALYISIMAVVFSTTINLTFDLSDITNYFIRMIMTL
                     LFIVLLIFLVNKGILPIAIPYRNNIQFYTTALDVLEDMKKKILRERKYKKRR"
     gene            complement(6832..7149)
                     /locus_tag="MJE33_002491"
     CDS             complement(6832..7149)
                     /locus_tag="MJE33_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648422.1"
                     /translation="MDFGNINLILIGIIVIIGTTIIYLIKPKTAFCSKKYFNKLESIY
                     GNIDKKKTVKLEVLYRYVTGLEYIAIGLFTRRLDITILAIILVAIITTALYYLIRKKY
                     ITI"
     gene            complement(7527..7727)
                     /locus_tag="MJE33_002492"
     CDS             complement(7527..7727)
                     /locus_tag="MJE33_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648423.1"
                     /translation="MYFKTYILKREVNNKQLEITRSFDLDNLICTATEILEDIEIDLI
                     IIDEKNNVVWRNEKNTPAHENA"
     gene            complement(7746..8159)
                     /locus_tag="MJE33_002493"
     CDS             complement(7746..8159)
                     /locus_tag="MJE33_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648424.1"
                     /translation="MKVKSKRSFIVGIIVCMLCCASLVIYCILKDKRFLISSFLLIVI
                     AIFNFCNAFSRKGIVEELHDSADERDLYLTMKTSHILVKIMNYTLFTFTFLFIIAYSA
                     WKNQSLLVIAITLCVIEIFLFVAYLLINIFLEKKE"
     gene            complement(8143..8376)
                     /locus_tag="MJE33_002494"
     CDS             complement(8143..8376)
                     /locus_tag="MJE33_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY9648425.1"
                     /translation="MKTKVREFRTNMGLTQQQLADLVHVSNRTIISIEKEQYSPSLML
                     AYRMAQVFGTTIEELCCLKENKEMEDKKHESKE"
     gene            8595..8969
                     /locus_tag="MJE33_002495"
     CDS             8595..8969
                     /locus_tag="MJE33_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBY9648426.1"
                     /translation="MKISKLPEAELKVMRYICEANKVLTSRELVAAMEEKYEWNESTT
                     FTVLKRLERREFLSTEKIGKLTHYNILVKEKKYLRFETKEFLKNIHKNSISSLMSALH
                     GEDDEVDEDKLSELEEYFKNLK"
     gene            9581..10273
                     /locus_tag="MJE33_002496"
     CDS             9581..10273
                     /locus_tag="MJE33_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY9648427.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     DIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIIGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
     gene            10469..12529
                     /locus_tag="MJE33_002497"
     CDS             10469..12529
                     /locus_tag="MJE33_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBY9648428.1"
                     /translation="MEKYRVLKLVSLIIISISFPLTILLVTIGLAEPKYNIQKTSTKS
                     GIFDISDDFYDSNIFDEGTMASLLPKFKLSVGGKLTSKEKGKFQQEGKSIDLSSSDSE
                     YDKIMINEQASEEYLSYLKNIKVYVINTYTGVFYTNTSYASLYDFKQKTKSYCNVEII
                     NKIGKSSYIKTINGEKFELNNLSLELNEVDEGFEAYVSFPEEPTIEDGIVYTNFQIFK
                     QATEKVRLFFVASLSLAIICLLSIILYIKIKGEVLKRNNVFLLIYSKIPMEFNVILVS
                     ISIAILTIYALAVSYHIDVIALVSLSLLIVSNNIFFINVQLSYLENKMCIFKTSIIVR
                     VILWLIKTVREVSNAIKKVSLARKVIVIVIISLALIMVISTRSIGMGLLFSICIFLGF
                     TIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDRAIEE
                     QVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYINVLESKSKRL
                     KLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPNDKV
                     YIWADGRRMYRVFENLLSNIAKYSLEETRVYIDVYDYGENVKVTMKNISSYELNFDPS
                     EIMERFKRADESRNTEGSGLGLAIARDLVALQGGKFYIEIDGDLFKANLEFQKYEEEI
                     TNME"
     gene            complement(12735..13058)
                     /locus_tag="MJE33_002498"
     CDS             complement(12735..13058)
                     /locus_tag="MJE33_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBY9648429.1"
                     /translation="MNNNYIFFIIIGMYIVTYLPRVLPMLIFSKKEMPDFLKKIMKFI
                     PVAILTSLTAKDVFFNGDSLYLSIANPKIISFMIVLLVAYKFKSIGISIITGVISIYL
                     FGIIL"
     gene            complement(13051..13752)
                     /locus_tag="MJE33_002499"
     CDS             complement(13051..13752)
                     /locus_tag="MJE33_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBY9648430.1"
                     /translation="MERTQQLKESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDRNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTSMFIALIVSQIKNKIYIVVFISSIIISVILYALFKSNLVIIVASILASLIG
                     FFIEESYCKKEESYE"
     gene            complement(14030..15415)
                     /locus_tag="MJE33_002500"
     CDS             complement(14030..15415)
                     /locus_tag="MJE33_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBY9648431.1"
                     /translation="MPINSFENYPMNWKPKRPSKGQILYKALAEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEMNTLFKHIIKEIGFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTSDSLLPASGGQNAIVAILAGLFQHGDRIGVDPLTYPGIKTAAKMLGIQLIPIK
                     QEDNEISEEGLLYACKNENIKGLYIIPDYQNPTTHIMSQNGRKMIANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSLSKIIAPSLRLAYISTPKQYREALSSALYN
                     INLSQSYFLTEIAYRMITSGEADKLINARRKSARRRNKIINQYLSGYNLLGNEECIFR
                     WLILPDGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESTEELKAGL
                     SVLKRLLEEKK"
     gene            complement(15523..16761)
                     /locus_tag="MJE33_002501"
     CDS             complement(15523..16761)
                     /locus_tag="MJE33_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBY9648432.1"
                     /translation="MELFIEVIKFNIYISPLILFFILFTKKISKYTFKFNYFICLLIV
                     VRMMFISKINLNILNINNTTINIYKHNIDNYIPYDQGITLDIFMYIWILGIIAISFYY
                     LRTNFKFYNYIENFKKEILEIDITSILDEQAKELSINKKLQIYKVKGIYSPAIIGLTR
                     CKIIIPEKKYNNKELRFIFKHELIHYKRKDNLFKLLISIICIIYWFNPILYLLKKYFH
                     ELCELSCDEIVIDKCTTSEIKEYAYIILDTIKYQKSLKYSSFVSQFLNKKNIILKKRL
                     NSMFNQDKKKINLGLKFLLTFIIIGSIFSINSNFKQNEDVEYTIGKINYNKNGETYGT
                     HIIDSNGNSIEPELIQTIGRGKKVGYVKKRDLYDCDNQPKTPEEAVLYTRKRNFNIFK
                     KTIPLYDKEGIKIIGRFEIN"
     gene            complement(16774..17142)
                     /locus_tag="MJE33_002502"
     CDS             complement(16774..17142)
                     /locus_tag="MJE33_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBY9648433.1"
                     /translation="MKKIPKSELLVMNFIWDKGIDKIPAREVANYMEISHGWTKGTTG
                     KVLSRLSDKGFLEFEKDGRNTLYTIKVKHEEYLKFETKDFLELIHKNSIHSFISSLGC
                     GNEISDDDIKELEQWLKKRK"
     gene            complement(17670..18092)
                     /locus_tag="MJE33_002503"
     CDS             complement(17670..18092)
                     /locus_tag="MJE33_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBY9648434.1"
                     /translation="MNTFKTEVLLKYSAKDIFNIFTKSARLNFPNFNEKKAVGTFVQQ
                     KNNKRFKVEITNFEKNRVYEIKTSNKRESTITRYELVHIDLENTKLIFIESESKRNIF
                     GKINSALVRVLFGKRQPEEFNKFIKKLEMELENNSVNL"
     gene            18570..21473
                     /locus_tag="MJE33_002504"
     CDS             18570..21473
                     /locus_tag="MJE33_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY9648435.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISAILQKNKNISISEITRELMTEGFNISRRVVEELLPKESLEM
                     DVVEEDEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIIGESGVGKSLFS
                     RHMYEFAKQKKIVKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDTPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRIPMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILAL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     IEYTEIRKYLKNAIFIPFNLEESSVKGSLLSDEYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSNEEIFSMSDLNSFVSDSLVKLVKELMNDIVEKNM
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     IGIKISDDEIGFITLYIKSALKNEVKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNISTKTID
                     RVDLLMALEATRKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIITLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDESLFCKIENLKETNNILAIVGTINPKIPGINFI
                     PYNKELFKNLDIYLSKSKQQDDRPIKSYERMLDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGAIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLEILKDAEFMNNANKCFDKDAFRRIIFDKFKHL"
     gene            21506..>21934
                     /locus_tag="MJE33_002505"
     CDS             21506..>21934
                     /locus_tag="MJE33_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY9648436.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSIMCKKEYVKETYINAVLERE
                     RTLPTGLDIGDMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLDVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNIKLLKNIKNASTKEEVAKLL"
BASE COUNT         8658 a         2366 c         3311 g         7599 t
ORIGIN      
        1 ctaaattaca aagagaatta tcaagaaaaa caatcggtag tttaaataga aataaagcaa
       61 gattaaaagt tgcaagactt caagaacata tagctaatca aagaaatgat tttctacaaa
      121 aactatctac taaattaata aaagagaacg atattatttg catagaagat ttacaagtaa
      181 agaatatgat taggaatcgt aaactatctc gctcaatttc tgatgtgtca tggtcagagt
      241 ttattcgtca attagaatac aaggctaatt ggcatggtag acaaattgta aaggtgggta
      301 aattctttgc aagttcacaa atatgcaata aatgtgggta taaaaacgag gaagtgaagg
      361 acttaaatat aagagaatgg atttatccta gttgtaatga aactcacgat agagatataa
      421 atgcaagtat aaatatatta aaagaaggat taagactaat aacaattcaa aataaataac
      481 gtatgtaaga accgtaggaa ctacggggat agcctgtgga gaattagtaa gacatatgtt
      541 agtatgcaaa attctgtgaa gcaggaaacc tgtgacttta gtcatgggag gttcaggcta
      601 gtattactca ttattgtatt caaaagctaa agtggaaacc tagtgaatat atgaatttag
      661 aagttaatga gagagcatta gcagccgact caatacttat taaaattgaa gatgaagaag
      721 aaacaatgaa agaagctgaa agagagaaaa agagaggtag aagaaggtag aatttaaagt
      781 taaaatagtt acattgctaa tatatgaaaa tgttacaaat ttggaaatag atggatatat
      841 atgttgtata gtataatgta gataatatat caccaagtaa aaaacaacaa ataaatctaa
      901 attatgaggg gtggaaaata aagatattaa acttacaata tgtatatttg cttttgtaat
      961 tggaataata gtgttatttt gctgtatagg tctaggtcaa tttaatatgg attgtattat
     1021 aaaacaaaat ggtgggtcaa tagctaccga acaatacaac atatatttag aacaatctat
     1081 ttcacaatat agaaattttg gttctatact ggttttacta ggaggaatag gaatactgtt
     1141 tgataaaagt gtcagaaaat actaaaaaag tatattttta tttaggatgt gatgaatgat
     1201 atggcaagag atgtatttag tgtaaagatg acaccactag aagcacagaa aatggtagaa
     1261 aaaaatttgg aagcagattt agtttctgtt gatatttata acctagaaaa taataagtct
     1321 ataatagtta caatctttga aaaatattat gcaagaagca atagtgatgc aggtttggta
     1381 gtgatttgtg aaaatactac aggaaaaaca cttgttaaaa ttacctcaac tggtagtgca
     1441 attggactat ttcagattga ttggggaaca ggtaataatt tgattaaaag agtaaaaaaa
     1501 atacttattg attatattat ttaattattt tatgtagata ttcttaggtt ggtatggaat
     1561 ttgtactaac ctattaagta atcaataaaa ctaaataaac atagaaagca cttacaaata
     1621 tgtaggtgct ttttattgaa aaaatagaaa ataaaataaa aagtaagata taggaaaaat
     1681 atgtaagaat tatatgttat aataattgta gcaagaagat gtaatctaca atttatagag
     1741 tggagttcat gtattaaaaa attatcctcc caacgataag aagggaggtg aatatacatg
     1801 gataattttt tatttaatgt gttagctagt gtaacagcta gttacatagt ttatttgatt
     1861 agcaagttaa tcaaaaaagt aaaaagccac tctaccgcaa agagtgactt aaagattgat
     1921 ataaatttta aatatcatcg taaaaaataa ctttcagtta tagaacttca ctctaccgca
     1981 aatagattgt agttcttctt gtttttatta taccacaaat tagaaaaaat attgcatata
     2041 atatttttta tagtcaataa aaatatgaaa ttttttatac acaatgatga tgaatctaac
     2101 tagatgaata aatttaattg taaataagaa agtacttact taaatggtag gtgcttttgt
     2161 tttgccaaat tatgctataa tattgagtga gatgagtaat ttgtggggga attttataat
     2221 gtggatatta gtaccaataa taacaatagt attacttata atagctgttt cttctatgca
     2281 atatatttta gttatgatag cttttttgtt gattatatat tcttttatag aaaagaaaat
     2341 agttatgggt cttgtgtcag ttcttttttt cacttattca atttatcttt gtgcaacttg
     2401 ggaagataaa tccttgatag cagataataa agttgagact gttagggcac aaagagaaac
     2461 tgtagaaaga gaaaaagaaa tggaaagaag aagaatacaa gaagaagtag acaaagaaag
     2521 atatattgaa aaacatggaa tggaaatatc agaaaatgat ttaaaagtta agctagaggc
     2581 tttagtacct caagaatata aggggaagaa atatgaatta aaagtaggaa aatttaaaag
     2641 atatagtatg tattttgatt taactgtaca aaatgaaaaa tttagtaatt ctgaagaatg
     2701 taaaaaattt gtaaaagaaa ttgctaatga tcttaaaaaa attaaaataa gcaaggcgta
     2761 ctttaagttt cattcaaaag atgacggcgg tatatataat tctgtttata ttgattattt
     2821 tagaaacata caaaataatg tagataatgt tgaaaattta gaattcaatg agtttgaact
     2881 taaaaccgaa gaagaagaga agcgagaaca agagaaaata gaacaagaaa aaaacagtta
     2941 taataactac attcaaaaca gagtggttga cccactagat agaataaaga aactaaaaga
     3001 gttgctagat tcaggagcaa ttacacagga agaatataat aaaaagaaaa aagaattatt
     3061 agaatagata atatagtaag cacttacaag tatgtaggtg cttttaaatt tacaaagtat
     3121 tccattttaa ttttatagtt tagattttat gatataataa aaatatagaa gttttgcagt
     3181 gtgcgatatt tgttacaaag tagggcttaa tacttgaaat ctaagatgtt gagggtgcgt
     3241 gataagtgtt atcaattgca ctattgcccg ctcactgcaa ttttaagagt attgtatata
     3301 tgtaggtatt ggaaatgcta agtttatttt ggggttttag attaactata tggaatgtaa
     3361 atattacatg ggtaaaaatt ttgatgattt ttatgacagt tttagattaa ctatatggaa
     3421 tgtaaattgt atagaattaa gaattttttt aatactaatt atagttttag attaactata
     3481 tggaatgtaa atattacatt taatttgcat tgctttgatg acttctttgt tttagattaa
     3541 ctatatggaa tgtaaatatt tttcaatatc aatatagaac tttcatcttt cccgttttag
     3601 attaactata tggaatgtaa atcaagatat ttttaataaa atttctgagc gaactgtttg
     3661 ttttagatta actatatgga atgtaaatga ggagattcag acagcgacta aaaagactga
     3721 tataaagttt tagattaact atatggaatg taaataactt tatatctcca tttgcatatc
     3781 catctgatga acgttttaga ttaactatat ggaatgtaaa tgcgtacaag gttgagaagc
     3841 tccgcaatct caacgtcgtg ttttagatta actatatgga atgtaaatat ttgaaaggaa
     3901 taaattttaa atgaatgaaa ggataggttt tagattaact atatggaatg taaatcagcc
     3961 aaaaacttcg aatttgaact aaaatgcgtg ggttttagat taactatatg gaatgtaaat
     4021 tctcatgact attgaataaa tataataaac aagcatacgt tttagattaa ctatatggaa
     4081 tgtaaattta gaatcaaaag cacttaatcc tagttctttc atagttttag attaactata
     4141 tggaatgtaa atagcttttt agcttcgtct actactttat aagcaaagcg ttttagatta
     4201 actatatgga atgtaaatga tgaacaataa aacaatcatc taaagacgaa gaaagtttta
     4261 gattaactat atggaatgta aatgttcctg ttgtgttttt tactaatccc attttatatg
     4321 ttttagatta actatatgga atgtaaattg ctgtgatttc actaataaaa caattaataa
     4381 ttgtgtttta gattaattat gtggattcaa aattaaataa acaaagaaag cacttacaaa
     4441 tatgtaggtg cttttattct gttcaaaatt ggtcggttgg gtaaaataat tagaaaaaat
     4501 tagtaaaaac ctcttttctg taactcgtta caatattatt attaatgtaa cgagttacag
     4561 aaaagaggtg aataaaatag caactaaaag tagggcagag tatatgaaaa atcgtcgaaa
     4621 agataaaaga ggttttagtg tacttttaga caaagaaaag ttagataaat ttgatgaagt
     4681 attagaggaa aagaatctaa ccaagaaaga atggctagaa gaaaaaatcg acgaggaact
     4741 ggaacaaaag gaataaaaaa taagggtcac tcccaccgac caaagtttga gtaaccccta
     4801 tgacgtatac tatcatatat caattatagt atatgtcatt ccttaaaaaa aatcaattat
     4861 taaggagtgt aatattatga aaaatgaatt aatgatgttt gaaggaaaag agattgaagt
     4921 atttgaattt gaaggaagaa ttttattcaa ctcaaaagat gtagcaaact gtttagacat
     4981 aaaaaatgtt aatgagaata taagacttat gaataaaaag caagttgtaa agttaagaaa
     5041 ttcggatatc agtaatactg acatccgaaa acttaataat gctggagaaa attttcttac
     5101 tgaaagtgga gtgtataaat taatctttaa atcaagaaaa gaagaagctg agagattcca
     5161 agactggata agtgatgaag ttttaccaag cataagacaa actggtgcat acataactaa
     5221 taatgcaatc ccgaaaagtt gagagaaaaa gcaagtgaga ttgaaaaatt acagttgctt
     5281 ataacagtac atctatgtta aaagaattgt tagatgatgc aggctttgac aataaatcaa
     5341 aactattaac agctaaaaca ttatataaga aagcaggaat tgatttacca atagagataa
     5401 acgaagaaga acattatttt gatacaaaac aaatagcatc taaactgaaa atatattcta
     5461 agagtaataa accagctcag atggctgttt gtgagattat taaaaagatt gatttagaag
     5521 atggtgaagt taaaggtgtt tggtaaacta atggttcttg gactggtact gtaaataaat
     5581 atacaaagag tgtaatagat aaggtgagaa cttggataga ggaaaataat agacctacca
     5641 agattgcagg tgagaagaag aattatcatg tatcttacaa aattgagtaa atttatcagt
     5701 tgtattaaat aatttagttt agttttgggg ggattaatac aatgtgtgag aatttacttg
     5761 ataatatgta tattgaaaag agaaaagaag aatatagaat taaacttttg aaaataagag
     5821 aaacagatat agatatatat aataagttag aaagtatagt atataaactt tctgagaaaa
     5881 aattagagaa aaataataaa tagacatata aagcacttgg atattctact gtttcaagtg
     5941 ctttgtttgg tataaaatgg tataatggag atagagaatt atattaaccc aaaaatggag
     6001 aaaatagaga agatgattta gttgacgctt tatcagtagg agtacttgca ggtaagacag
     6061 aatctctagt actattagtt ggaaatggaa taagtgatac tcaaaaaagt ttaataaata
     6121 aaagagaatt tagaaatata actcaaattg gtggaaatgg aaatgagaaa gcatttagtg
     6181 aagtagaaaa cttagttaaa tagattagaa ggtagcaact agagttggtt gttaccttct
     6241 ttttttatac tttctctctc taagtatttt ctttttcata tcttctaata catctaatgc
     6301 agtagtataa aattgtatat tatttctata aggtatagct attggaagaa tgcccttgtt
     6361 aactaaaaaa ataagaagta caataaataa aagtgtcata atcattctaa taaaataatt
     6421 agtgatatct gataaatcaa atgttaagtt aattgttgta gaaaatacaa cagccataat
     6481 agatatatac aaagcaaaaa tggggttact atttttttgt aactgtcctt taagcctaga
     6541 ttgctctatt tctatatctt gaatactttt tatggaatcc atttcactat aatacttata
     6601 aacttttaaa taatattttt tatacttgtt taaagcttct tcattatctt taaggtcttt
     6661 gtcacttaac tctgatttaa cttccccaaa atccataaaa tctctcctac aatgttatat
     6721 agtatacttt aatttatagc atatttaaga acttatatca gcaatttgtc gaacaatttt
     6781 agctaaaaat agagtataat gataggctta ttatttataa gatattattt tttatatagt
     6841 tatatatttc tttctaatta gataatataa tgctgttgtt ataatggcta ctaatattat
     6901 tgctagtatt gtaatatcta atcttcttgt aaataagcct attgctatat actcaagacc
     6961 tgttacataa cgataaagta cttctagttt aacagttttt ttcttatcta tatttccata
     7021 tattgattct aatttattaa aatatttttt actacaaaaa gcagttttag gtttaattaa
     7081 atatattatt gtagttccta ttataactat tattccaata agtataagat tgatatttcc
     7141 aaaatccata gcagatacct cctattaaat ttgcttttct tagtcaaagt atatcagtaa
     7201 cacaagtaca aagagtatat aaaatattac aaatattaac aggatagata aaactatagt
     7261 aaaaattgta ataataaagc gaacgaaaat tatcatacta aaacataaga catgttataa
     7321 ttgtattaga tagaagatta acatacacca aagcactcct tataataaag agtgcttatt
     7381 tttttgaaat tcatcaatat ataaactatc aagaatctta ttcaacacac cttaaaatcg
     7441 atttaaatag ctctttttga tagcataatt tatatgatat aataaaaaaa tagaagtgta
     7501 ttcgcagtac acttctatag ttataactaa gcattctcat gagcgggagt gtttttttca
     7561 tttctccaaa caacattatt tttttcatca ataattatca aatcaatttc aatatcttct
     7621 aaaatttctg ttgcagtaca aattagatta tctaagtcaa aacttcgagt tatctctagt
     7681 tgtttgttat ttacttctct ttttagtata taagttttaa aatacattta tatcacccct
     7741 tgtatttact cttttttctc taaaaatata ttgataagta aatatgcaac aaataaaaaa
     7801 atctcaatga cacatagtgt tatagctata actagaagtg attgattttt ccatgcacta
     7861 taagctatga tgaaaagaaa tgtaaaagta aataatgtat aattcataat tttaactaag
     7921 atatgacttg ttttcattgt taagtacaag tctctttcat ctgcactatc gtgtaattct
     7981 tcaacaatgc cttttcttga aaaagcatta caaaaattaa agattgctat tacaattaaa
     8041 agaaatgatg aaattaggaa tcttttatct tttaaaatgc aataaataac taaactagca
     8101 caacataaca tacaaactat aatacccact ataaaacttc gtttactctt tactttcatg
     8161 ttttttatcc tccatttctt tattttcttt taaacaacat agttcttcaa tcgttgtacc
     8221 aaacacttgt gccatacgat aggcaagcat aagagaaggg ctatattgct ctttttcaat
     8281 agaaataatt gttctgtttg atacatggac taaatcagcg agttgttgct gtgttagacc
     8341 catatttgtt cgaaattctc gtacttttgt tttcattcca taacctcttt ctatatgaag
     8401 ctaacttcat gtgaagttaa cttcataata tcctaaaatg agtaaaatgt caatctcatt
     8461 agataagtat tataaaacct aaaagaataa tatggtaaaa attggatgga attatttacc
     8521 ttaattctct tgtttgtaaa actttattga tatagaattt aacatataca taaatcggca
     8581 aaggggtgcg aattatgaaa attagtaaat taccagaagc ggaattaaaa gttatgagat
     8641 atatttgtga agctaacaag gtattaactt ctagggaatt agttgctgca atggaagaaa
     8701 aatatgagtg gaatgaatca actacattta cagttttgaa aagattagag cgaagggagt
     8761 ttttaagtac agagaaaata ggcaaactta cacattataa tatactagta aaagagaaaa
     8821 aatatctaag gtttgaaaca aaggaatttt tgaagaacat acataaaaat tcaatttcaa
     8881 gtttaatgtc agcgttacat ggtgaagacg acgaagtgga tgaagataaa ttaagtgaac
     8941 tcgaagaata ttttaagaat ttgaaataag aaaatgacaa attgatacat aataaataaa
     9001 atgaaggtat gaaattataa ctttttctac cttcattttg tttattattc tgaacatgtt
     9061 aagaaaatga aataagtttg tattttagtt atataaattt taatagaata tgtacagtat
     9121 atagtagata gaatgtataa aaatagaatt taaataagta gctattattg cagtgaaatg
     9181 aatgtttcta agtatatttg ggtggaagaa tggtggaaga aagttagtga aatgtaattc
     9241 ttccaccatt cttccaccca aaaaataaaa actgttcaat aagaattaat atacatcaat
     9301 tcagctgaat gcaatcaaaa tacttaaatg aaagtaatct gaaaacaaca taattcaaat
     9361 caatttattc aaataaacct caatttatct taaatcttaa gaaatattta agaattagga
     9421 taatattttc ttaagattta tattttatta tatagttata agggaaagtt acaaaaatgt
     9481 aagccaaagt aatataacaa tttagtcatt gttgagaaag ggagtgaaag atattgtata
     9541 atatagataa gtattattat acaatagagg aagtgaaaat atgtacaata tattagtagt
     9601 agacgatgat aaagaaattg ttgattcgat agaaatatat cttaaatcag aaggatttaa
     9661 aatatataaa gcatatgatg gaatgaaagc tttggaaata ataacagaga atgacattca
     9721 tctaatacta atggatataa tgatgcctaa attagatgga atcaaagcaa ctgtaaaaat
     9781 acgagaagaa agaaatattc caataatttt aatttcagca aaaagtgaag atacagataa
     9841 aataatagga ttaaatattg gagctgatga ttatataaca aagccattta atctattaga
     9901 gcttatagct agggttaaat caaacttaag acgatatgta actttaggta attatgaaga
     9961 tacatgtaaa gaaattttaa aaagtggagc tttggagtta aatacactaa caaaagaagt
    10021 taaaattgat ggtgaaaaca taaaaatgac tccaattgag tataaaataa ttgaacttct
    10081 tttagaaaat aaaggacgag ttttctcaat agatgagata tacgaaaaag tatggaatga
    10141 ggaaagtttt aattcagaaa atacagtagc agtacatatc agacgaataa gagaaaagat
    10201 tgaaataaat ccaaaagaac caagattttt aaaggtggtg tggggagttg gttataagat
    10261 tgaaaaattt tagtggaaaa attataaaag aaaagagttt aatatttaat tgcattttga
    10321 ttttagtcat ttttacattt acacaaatgt taggaatttt aatatgtttt aattttttct
    10381 ctgagagttt aatttactct atggggatta tgcttttctt aaactttatt ttatataaaa
    10441 tactgacaat gtagatgagg tgggcaactt ggagaaatat agagtattaa agttagtttc
    10501 attaatcata atatctatct catttccatt aactatattg ttggtgacaa taggacttgc
    10561 tgagcctaaa tacaatatac aaaaaacttc tacaaagagt gggatttttg atatatctga
    10621 tgatttttat gatagtaata tatttgacga aggaacaatg gcaagtctat tacccaagtt
    10681 taagctatct gtaggtggta aattaactag taaagaaaaa ggtaaattcc aacaagaagg
    10741 taaaagtata gatttaagta gtagtgattc tgagtatgat aaaatcatga ttaatgaaca
    10801 agcttctgaa gaatatttaa gttatctaaa aaatataaaa gtgtatgtta ttaatacata
    10861 cacaggagta ttttatacaa atacgtcata tgctagttta tatgatttta aacaaaaaac
    10921 taagagctat tgtaatgtag agataataaa taagatagga aagagctcat atattaaaac
    10981 aataaatgga gaaaaatttg aactgaataa tctgagtctg gaactaaatg aagtagatga
    11041 aggttttgag gcatatgtat cttttccaga agagccaact atagaagatg gaatagtata
    11101 tactaatttt caaatattta aacaagctac agaaaaagta agattatttt ttgtagctag
    11161 tttaagttta gcaattatat gtttattatc tataatttta tatatcaaaa taaaaggtga
    11221 agttttaaaa agaaataatg tatttttact aatatacagt aagataccta tggaatttaa
    11281 tgtaatatta gtatctattt caattgcaat tttaactata tatgctcttg ctgtatctta
    11341 tcatattgat gtaattgcat tagtttcttt atctttactg atagtatcaa ataatatatt
    11401 ttttataaat gtgcaattga gttatttaga aaataaaatg tgtatattta agacaagtat
    11461 tatagtaaga gtgattttat ggttaataaa aactgtaagg gaggtcagta acgctataaa
    11521 gaaagtatct ttagcaagga aagttattgt tatagtgata atatctttag ctttaatcat
    11581 ggtaattagc actcgaagta taggtatggg attactgttt tctatatgta tttttttagg
    11641 atttacaata tatataacta aaagactttc atatcttagt tacgtaatag aaggtacaga
    11701 aagaataaaa aagggagaat tagattataa gataaaaatt actgggaatg ataattttac
    11761 gtctttagct gaaaatataa ataatattgg agaaggtctt gatagagcta tagaagagca
    11821 agtaaaaagt gaaagaatga agtctgacct tataacaaat gtatctcatg atttgaaaac
    11881 tccacttact tctataataa attatgtaga tttaattaaa aaagaagaat caatacaacc
    11941 agagtatata aatgattata taaatgtact tgagtcaaag tcaaaaagac taaaattatt
    12001 gatagaagat ttatttgaag cgagtagagt aagtagtggc aatatagaat tacaaattag
    12061 caagattgat ttagtgcaac tattaagaca aagtataggt gaacttgaag aaaaattatc
    12121 taaaaataat ttatatttaa aattaaatgt tccaaatgat aaggtgtata tatgggcaga
    12181 tggaagaaga atgtatagag tatttgaaaa tcttttatct aatatagcaa aatattcctt
    12241 agaagaaact agagtctata ttgatgtata tgactatggt gaaaatgtaa aggtaactat
    12301 gaaaaatata tcttcttatg aattaaattt tgacccaagt gagattatgg aaagatttaa
    12361 aagagctgat gaatcaagga atactgaagg aagtggatta gggcttgcta ttgcaagaga
    12421 tttagtagct ttacaaggtg gaaaatttta tatagaaata gatggagatt tatttaaagc
    12481 aaatttagag tttcaaaaat atgaagaaga aataacaaat atggaatgag atagtattta
    12541 agtatatata ttttttttac tgtcgaaagc ttttattata atatattaga atatttacag
    12601 tagagaatga agagtaaaaa cagagtaact ttaagtcact ctgtttttta tttaaaatca
    12661 ataatataac aacttaaatt aaaattgtac acatagtttt aaaataactt atacaataaa
    12721 acattatcca aatatcacaa gataattcca aataaatata tagatatcac tcctgtaata
    12781 atagaaatac caatagattt aaacttatat gcaacaagaa gtacaatcat aaaagatata
    12841 attttagggt tagctataga taaatataga ctatctccat taaaaaatac atctttagca
    12901 gttaaggaag taagtattgc tacaggtata aatttcatga tttttttcaa aaaatcaggc
    12961 atctcttttt ttgaaaaaat taacatagga agcactcttg gtagatatgt aacaatgtac
    13021 attccaatta taataaaaaa tatataatta ttattcataa gattcctcct ttttacagta
    13081 agattcttct ataaagaaac caattaaaga tgctaaaatt gatgcaacta ttattactaa
    13141 attgcttttg aataatgcat ataaaataac agaaataatg attgatgata taaatactac
    13201 aatataaatc ttatttttta tttgagaaac tattaaagct ataaacatag aagtcaatat
    13261 gaagccagaa atagaactgt ctatagatat actttctcct aaaaatgctc caataaaatt
    13321 agctaaaact gatgaaatta agcagaagaa attaagataa atagcatttc tatcagacca
    13381 atttccagca gtaaattttt cataattaac tgcaaaagtt tcatctgtta tagtatgaca
    13441 aaatggcaat ataaattttg gacttttatt ttttatgtac atgttcaaat tagaactcat
    13501 tagaaaatgt cttaagttaa ctataaatgt agtcaaaaca atagatgtta cacttgcacc
    13561 agtagaaatc attgaagctg caatgaattg agaacttcct ccaaatacta aagttaccat
    13621 tagtgctatt tgaaaaggtg aaagaccaga tttttgtaat agtattcctc ctactatacc
    13681 tataggtata taacctaaga atataggaat tgaagcaata aagctttctt ttaattgttg
    13741 agttctttcc acaataaatc actccttttg tattaaatgt attaaagtat tcgtaaaaca
    13801 gaaaaataga ttaattgtat aattgcaatt ataattaaaa ataaatttgg attaatatat
    13861 aaaatactat acaacaaata gataatctta aacggttatt aaattttatc tatttgttat
    13921 tatgatgtaa taatacatat taatctctat aagttaatta ctatctggtg taattttatt
    13981 ttaacaacaa aaaaaaatac acacaattaa aatattgtgt gcaatacaat tattttttct
    14041 cttctaatag tctttttaat acgctcaaac cagctttaag ttcttctgta ctttctgtag
    14101 cacatacagc tattcttatt gcagaaattg gtttttcttt tccaacagca aaacgttcag
    14161 aagcatagac ctgtacacct tctttaagtg cttgtatctc aaatttttct gccattattc
    14221 catcaggtag aatcaaccat ctaaatatac attcttcatt tcccaaaaga ttatatccag
    14281 ataaatattg attaataatc ttgtttcttc ttcttgcaga tttacggcgt gcattaatta
    14341 atttatcggc ttcacctgat gtaatcatgc gataagcaat ttctgttaaa aaataagatt
    14401 gagatagatt aatgttgtat aatgcgcttg ataatgcctc tcgatactgt ttaggtgtag
    14461 agatatatgc tagccgtaaa ctaggtgcaa taatttttga taaactcgta atataaattg
    14521 tttgattggg cagataggaa gccactggat ttaaatgtgt ttcattaagt aaactatgaa
    14581 tagcatcctc aataacaatt aaattgtatt tagatgcaat atttgcaatc atttttcgtc
    14641 cattttgtga cattatatgt gtagttggat tttgataatc tggtattatg tatagacctt
    14701 taatattttc atttttacaa gcatataata gtccttcttc actaatctca ttatcttctt
    14761 gtttaattgg aattagttga atcccaagca ttttagctgc tgtctttatt ccaggataag
    14821 ttaatgggtc tacacctatt ctatctccat gttgaaacaa accagctaaa atagcaacaa
    14881 ttgcattttg tccaccactt gcaggaagta agctatctga tgtagtttca agcccagctt
    14941 tataaattaa tttggcaata gcttcttttt gccatttagc accatctctt tgtccatatt
    15001 ggaatatggt tttaaaacct atttccttaa ttatgtgttt aaacaaagta ttcatttctt
    15061 ctagcgtgaa atctggattc attgtgccca tttcaataaa tgtaatttta ttattactgg
    15121 acatcaagaa tagatttgaa cgtgtatcat aagatacaaa agttccactt cctgtaactc
    15181 cacttattag accttttttt tcacaaattt taaaagctct agagatagta ctcacattaa
    15241 catctaagaa atctgccaat tcacgttggg gtggtaattt agtacctggt aataaaaatc
    15301 cattgttgat atcttgttct agttgttctg caagcgcctt atataatatt tgcccttttg
    15361 aaggtctctt tggtttccag ttcataggat aattttcaaa agaattaatt ggcataagtt
    15421 ttcacctcac attattaatt tgattttttc tttatctaaa acaataaatt ctaaagatac
    15481 aaataaaaac tgataggaaa taattctatc agtttttgtc ttttaattga tttcaaatct
    15541 tcctataatt tttatacctt ctttatcata aagagggatt gtttttttaa atatattaaa
    15601 atttcttttt cttgtatata aaacagcttc ttctggtgtc tttggttgat tatcacaatc
    15661 gtacaaatct ctttttttta cgtagccgac ttttttacct ctacctattg tttgtataag
    15721 ttctggttca atggaatttc catttgagtc tattatatgt gtaccatagg tttctccatt
    15781 tttattataa ttaatcttac caatagtata ttctacatct tcattttgtt taaaattgga
    15841 gttgatagaa aatattgacc ctattattat aaaggttaat aaaaacttaa gacctaaatt
    15901 aattttcttt ttatcttgat taaacataga atttaacctc ttttttaaaa taatattttt
    15961 tttatttaga aactgagata cgaatgatga atacttcaaa gatttttgat actttattgt
    16021 atctaatatt atatatgcat actctttaat ttcactcgtg gtacacttgt caatcacaat
    16081 ttcatcacaa gataattcac ataattcatg aaaatatttt tttaataagt ataaaattgg
    16141 attgaaccaa tatatgatac aaattatact aattagaagc ttaaataagt tatcttttct
    16201 tttataatga attaattcat gtttaaagat gaatcttaac tctttattat tgtacttttt
    16261 ttctggaatt attattttac agcgtgttaa acctataatg gcaggagaat aaataccttt
    16321 aaccttatat atctgaagtt ttttatttat acttagttct tttgcttgtt catctaagat
    16381 actagtaata tctatttcta aaatttcttt tttaaaattt tctatgtaat tataaaattt
    16441 aaaatttgtt cttaaatagt aaaaagagat tgctataatt cctaatatcc agatgtacat
    16501 aaatatatct aaagtaatgc cctggtcata tggtatataa ttatcgatat tatgcttata
    16561 gatattaata gtagtattat tgatatttaa tatatttaaa tttattttag atataaacat
    16621 cattctgact actattaaaa gacatataaa ataattaaac ttaaaagtat atttgcttat
    16681 ctttttagta aataaaatga aaaaaagaat tagtggactt atgtaaatat taaatttaat
    16741 aacttctata aataattcca taaaatacgt ctcctatttt ctttttttta gccattgttc
    16801 aagttctttt atgtcatcat ctgatatttc atttccacaa cctaatgatg atataaagct
    16861 atgaatagaa tttttatgta taagctctaa aaaatccttt gtttcaaatt ttagatattc
    16921 ctcatgttta actttaatag tgtataaagt atttctacca tctttttcaa attctaaaaa
    16981 acctttatca cttaatctag aaagtacttt accagtagta ccttttgtcc atccatgaga
    17041 aatttccata taattagcta cttctctggc tggtatttta tctatgcctt tatcccatat
    17101 gaaattcata actaacaact cggattttgg tatttttttc aatataatca ctcaccttta
    17161 acttgattat attagacaaa tgtctaatag tcaatataat atatcatcat gtataaataa
    17221 tctttatatt attttatgct tgagaactaa gtagtataga tttatagata aaaaagatat
    17281 tgagagtttt atggaagagg ggaatatatt ttctattaga gataagaata tgacatatat
    17341 tttttattga tttgtacaaa aatactatat agtgaataaa ttaatatctt ttctaaatta
    17401 gaagaggaga atcttattag ataaagatta tcctctttat tgaaagttta agggatatta
    17461 gagattatta atttttctct ctttttttaa ctaaaagcaa taatacataa aattattatt
    17521 gctaatgata ctcttaatgt ggtatatagt catagtgtca catgtttatc aatagttttt
    17581 tcattaatta ttgtaacgaa tagtaggact cataaaatac ttagaaatat gtttttagat
    17641 agattttaaa tatataatct aacttatatc tataaattaa cactattatt ctccaattcc
    17701 atttctagtt tttttataaa tttattaaat tcttctggtt gcctttttcc aaacaatact
    17761 cttacaagag ctgaattgat ttttccaaat atattcctct tgctctcact ttcaataaaa
    17821 ataagttttg tattttctaa gtcaatatgt actaattcat atctagtaat agtactttct
    17881 cttttattgc ttgtttttat ctcatatact ctattttttt cgaaattagt tatttcaacc
    17941 ttaaatcttt tattattttt ttgctgtaca aatgttccta ctgctttttt ctcattaaaa
    18001 ttaggaaaat ttagtcttgc agattttgta aatatattaa atatgtcttt tgcagaatat
    18061 tttaataaaa cttcagtttt aaatgtattc atatcgtttt cccttctgta tataaattta
    18121 atatttataa gttaattttt aagttaaaat agtataatat gccatattaa atcatgttaa
    18181 aactatttat atagctaaaa tcttacctat ataagtatgt atactatcat ataaaaataa
    18241 aatcttaaaa tagaaagggt taaaaacaca gtttattata atttttattg ttgagtagat
    18301 acgtactatt atatatgatt gagcaagttt ttcaatcata aatttataaa gtattttgca
    18361 ttaaaattta aacaaagaat taaattattt aagattatga ataactaata catttagtaa
    18421 attactaaat atacaaaatg taaatactta tcaaaatgta taaatactta tgacatctag
    18481 ttacttacag ggtttaagta gaaaaaaata tcaaagtaag tatggcatga tagttgctta
    18541 tatatttatc aagaaaggag ttattaagca tggagttgag agataaactt gtaaacatca
    18601 taaatacaga aaataaaaaa gatccattaa cagatgttca aatagcaaag tttttaagta
    18661 caactagaga gaacataact aatttgagaa aagaacttaa tataagtaat tcaaggcaaa
    18721 gaagatatcc ttatttaaaa tcagcaattt cggcaatatt acaaaagaat aagaatattt
    18781 ctatatcaga aattacaagg gaattgatga cagaaggctt taatatatcg agaagagttg
    18841 tggaggaact tttacctaaa gaaagtttag aaatggatgt agtagaggaa gatgaagaag
    18901 aatcgaatga cccatttgaa actctgatag gtaataaagg gagtcttaga aatgctgtag
    18961 agcaggctaa atctgctata ttgtatccac caaaagggtt accaacgttg attattgggg
    19021 aaagtggagt tggtaagagt ttattttcta gacatatgta tgagtttgca aagcaaaaaa
    19081 agatagtaaa agagagctcc aattttgttg tatttaactg tgctgattat agtgacaatc
    19141 cacaattatt attatctttg ttatttggat ataaaaaagg tgcatttaca ggtgctgaat
    19201 atgatacacc aggtcttgta gaagaagctg atgaaggagt tttatttttg gatgaaatac
    19261 atagattgcc tccaaaaggt caagaaatac tattttctat cttagatagg ggaaagttta
    19321 ggagattagg tgaaactaat gctgaaagaa aagtaagtat tatgttgatt ggggcaacta
    19381 ctgaaaatgt agagactaat ttattattaa cattcagaag aaggatacca atgctaataa
    19441 ctttgccacc attacatgat agattactaa aagagaaaat agaccttata tacaatatct
    19501 ttcaacaaga gtgtaataga ataaatgcaa aaatatttgt agataaaaat gtgattgaaa
    19561 tacttgcact aaaaaagttt agtggaaata taggacaatt acaaaatatg attcaggttc
    19621 tttgtgcaag agcttttatg aagtttataa attccaaaga tgaagatagt gaatctatag
    19681 tagtagtaga tataaatgaa gttttaaagc ttaaagattc gtttaaagat gctgcctttc
    19741 aagagattga atatacagag ataagaaaat atttgaaaaa tgctatattt ataccattta
    19801 atttagaaga atcatcagta aaaggaagtc ttttaagtga tgaatatata ttaccagagg
    19861 acatatacaa aacaattgaa aagaaatatt atgacctaaa atccctagac attgaagata
    19921 tagatataga gaatatttta tggactttta ttttaaatag atttgctgac ttaaagttta
    19981 atcttgatag taatgaagaa attttttcaa tgagtgattt aaatagtttt gtaagtgata
    20041 gtttagttaa actggtaaaa gaacttatga atgatatagt tgaaaaaaat atgaatttag
    20101 aagtaaataa aaatatgttc aaatatctag caatacactt agaagaagct ataaaaagaa
    20161 ttaagctaaa tcaaaagata ataaatgtaa atttagaaaa aataaagatt gacttttcaa
    20221 aagaatatga aattagtaaa agttttgcaa taaaaattga ggaatcaatt ggtattaaga
    20281 tttcagatga tgaaattgga tttataacgc tctacataaa gtctgctcta aaaaatgaag
    20341 tgaaaaaaga taaagtaggg ttaataataa tttctcatgg acgtatagct acagagactg
    20401 taaatgtagt taaagaactt ttaggtgtaa agtttcctgt ggctatagat atgcctttag
    20461 atgaaaaacc aataaatata tacaataagg cagtagaatt gtcaaaaata atagaccaag
    20521 ggaaaggcat attgtttttg gtggatattg gttcacttac aaacataggt caaatagtaa
    20581 ataagagaac caatataagt actaaaacta ttgatagagt agacctccta atggcactag
    20641 aagcaactag aaaagtttct atgggagagg aagaacttga tgaaatattt ttttctttgc
    20701 taaaagatag gatgggatat aactatgaat tagaaaaaca catagataaa tcaaatgcaa
    20761 ttataacatt atgtcttact ggagaaggaa ctgctaagta tataagtaaa acgttggaag
    20821 aaaagtatga taacactaaa tgttatcaaa tgagtgcatt ggatgaaagt ttattttgta
    20881 aaattgaaaa tctaaaagag acaaacaata tattagctat tgttgggaca ataaacccta
    20941 agataccagg tataaatttt ataccctata ataaagaact tttcaaaaat ttggatatct
    21001 atctatcaaa atcaaaacaa caagatgaca gaccaattaa atcgtatgaa agaatgcttg
    21061 atgaggattt agtgattttt gaaccagata tatattttaa aaaggattta ttagaatatg
    21121 tatgttcaat tcttataaat aaagaatatg tagaaaaaga ttacttagat tctgttttac
    21181 atagagaaga aatgcttcca acatattcta aaggagctat aggtgtgcct catggagatt
    21241 cttctactgt aaatagtaca aggtttgttt ttgttaaact aaagaatcct attgattggg
    21301 gtgtaggtaa tgtaaatttc atatttatgc cagtttttca ggctaatgat aaagagattg
    21361 ttaaaaatgt tcttgagata ctaaaagatg cagaatttat gaacaatgcc aataagtgtt
    21421 ttgataaaga tgcttttaga aggattatat ttgataaatt taaacatctg tagccatagc
    21481 aaaaaaattg agaggagtta gagtaatgaa tgtagattta atatcattgg gtttagaatc
    21541 taatgattat gaaagtgtta ttgaagaatt aggaagtata atgtgtaaaa aagaatatgt
    21601 aaaagaaaca tacataaatg ctgtcttgga aagagaaagg acattaccaa caggtctaga
    21661 tattggagac atgtgtgttg ctattccaca tacagattca aagcatgtaa atgagtctaa
    21721 tgtagctgta gggatattga aaaatcctgt aaagtttaat tcaatgatag acccaaaaga
    21781 tagacttgat gtagaacttg tatttttatt agcagtaaag aacccagact cacaagtaaa
    21841 actgttaaaa gatttaatgt cagtttttca aaatataaag ttattaaaaa atataaaaaa
    21901 cgcatcaaca aaagaagaag tagctaaatt attg
//
LOCUS       DAGSRM010000063        21706 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.063, whole genome shotgun sequence.
ACCESSION   DAGSRM010000063 DAGSRM010000000
VERSION     DAGSRM010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21706
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.063"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            111..1541
                     /locus_tag="MJE33_002506"
     CDS             111..1541
                     /locus_tag="MJE33_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY9648437.1"
                     /translation="MSSQESKPKGNLISNFRDDMRDPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAVAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIEDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNQRNA"
     gene            1644..2747
                     /locus_tag="MJE33_002507"
     CDS             1644..2747
                     /locus_tag="MJE33_002507"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBY9648438.1"
                     /translation="MIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKEIIKLASNENPL
                     GPSPKAIEAMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVANGADNVITLIGE
                     AFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGILDKITDKTKLIC
                     VCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVDGLKYVRENYNV
                     IVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGATAALDDEEFIK
                     ETYELNRVGMAYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDLKKRGFVIRPSA
                     THARVSIGTMEENRKFVAVLKEILNIKGEPSIG"
     gene            2762..2998
                     /locus_tag="MJE33_002508"
     CDS             2762..2998
                     /locus_tag="MJE33_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBY9648439.1"
                     /translation="MKRGVDMNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAH
                     VEVDESKCILCGICYQVCPDNVFEIIKEEACQEA"
     gene            3015..4064
                     /gene="vorB"
                     /locus_tag="MJE33_002509"
     CDS             3015..4064
                     /gene="vorB"
                     /locus_tag="MJE33_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBY9648440.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVMEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDVAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREGDLGWTYDGSNRDHAKVADNQKPTFCMEKRM
                     RITENEQQWEDYQVEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVSKDVKGFISVESTDTGQLVEDVALASKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            4066..4806
                     /locus_tag="MJE33_002510"
     CDS             4066..4806
                     /locus_tag="MJE33_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBY9648441.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSVNGRDIKTTGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWGV
                     SLEKSIKWMEEEIVPYYALGEFKQRDGE"
     gene            4809..5348
                     /locus_tag="MJE33_002511"
     CDS             4809..5348
                     /locus_tag="MJE33_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBY9648442.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDVLVAMNKPSLDYINLVKEGGTVVINTSAIDENVT
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLFEKELALKTMCDFFEEKGK
                     GKFNVQNEAAFMEGYNNAI"
     gene            5472..7571
                     /locus_tag="MJE33_002512"
     CDS             5472..7571
                     /locus_tag="MJE33_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HBY9648443.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKETDRVYYVN
                     PKREELFGRKCYKTIQEVPEVVDCVVVCTPRNVVPSVLKDSGELGTKAAVVYASGFAE
                     EGTEEGTDLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWDLYTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDDYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPVIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPANSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMKKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDDSVGFTMVGANIRDEE
                     NEMHETLCKAVSEAREQGLKKPVFAVPTLEGNRYLDYRTRLEDNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNAKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIKNRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPLLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSEPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(7838..8353)
                     /locus_tag="MJE33_002513"
     CDS             complement(7838..8353)
                     /locus_tag="MJE33_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HBY9648444.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDSDFTSAMDILERTLAHEKKVTALIN
                     NLAAVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YAAPTNPPVTI"
     gene            complement(8445..9656)
                     /locus_tag="MJE33_002514"
     CDS             complement(8445..9656)
                     /locus_tag="MJE33_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBY9648445.1"
                     /translation="MSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTKM
                     TLSNLVTELINENMIREIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVII
                     SDLKGNIVKQLSETYPKCLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDIN
                     GTILNPPDFGNISNLNIVDFIKEMSDLPIFLINDANSGALAEKMYGLGKNISNYIYLH
                     IMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSVSNLIKR
                     IESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLID
                     CSSIIIGYDFNIPGTFIENTLLKKLTASASFSKYKKISVRHSNFGANAPLIGSIAIVS
                     YNFFNGSINFY"
     gene            10332..11774
                     /locus_tag="MJE33_002515"
     CDS             10332..11774
                     /locus_tag="MJE33_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBY9648446.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPNETKQRLKNVKKIY
                     IIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVNGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSAAQNNGDSSTKGESPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
     gene            11816..12169
                     /locus_tag="MJE33_002516"
     CDS             11816..12169
                     /locus_tag="MJE33_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648447.1"
                     /translation="MKKSNIFSIIFPTVIMVLMFLTFATDIFNIPDIQSKSIFVVGMV
                     LIFPISFLIQGIICVLNKTNWILSLIVSLVTYIVLMYIFLNDSAYIYVLLYTAFYMIG
                     HITAKLYYKIKTFKN"
     gene            12360..13181
                     /locus_tag="MJE33_002517"
     CDS             12360..13181
                     /locus_tag="MJE33_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBY9648448.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEEIINSNPFDFVL
                     CSTHTIDNVPVPSKEYFKGLSKEDAYYKYFNSILETNREFGDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDVIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFVNIEKVKSNNIA"
     gene            13439..15322
                     /locus_tag="MJE33_002518"
     CDS             13439..15322
                     /locus_tag="MJE33_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBY9648449.1"
                     /translation="MSNKNKENITLSNQIKGTIQNSLTKEAILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGGGNLNASGVGWSGGTNIVMSKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQEEKLLFTEKRIIELGNENDEKELCEIGNFFGYPY
                     LNYIDIPKVPNWVKDTVWYQIFPDRFANGDPSINPEGVDKWGAIPTRDNFTGGDLQGV
                     IEHLDYLSDLGINGIYFCPITIGKTNHRYDTVDYMEVDPTLGDKETLKKLIEEAHKRN
                     IRIMLDAVFNHIGYYSKQWQDVVQNKENSRYKNWFYIKDMSKIDTPIEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVIKYLLDVGKYWIQEFDIDAWRLDVSNEVDHVFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTDEINAEEFKYMIND
                     VMVSYPIQVSEVIFNLLGSHDTTRILTYANGNIDRFKLAYMFMFVQTGCPCIYYGDEI
                     GMEGELSSISEGQRKCMKWNEEKWNKDIFDFMKKIIRLRKENKELRSISNEWVLADKE
                     NGTIILKKESISIIINNSSKNIILTLPDYLANKKVKDLYEEEIVDLKEELMLEKYKFI
                     ILK"
     gene            15547..16227
                     /gene="pgmB"
                     /locus_tag="MJE33_002519"
     CDS             15547..16227
                     /gene="pgmB"
                     /locus_tag="MJE33_002519"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBY9648450.1"
                     /translation="MIEAFIFDLDGVITDTAYYHYMAWRKLAHKVGIDIDTKFNESLK
                     GISRMESLDRILEFGNKKYSFSEEEKVRMAEEKNNYYVSLIDEITSNDILPGIESLLI
                     DVKSNNIKIGLSSASKNAINVLNHLGISDKFDFIADAGKCKNNKPHPEIFLMSAKGLN
                     VNPQNCIGIEDASAGIDAINSANMFSVGVGNYENLKKANLVVDSTNQLKFEYIQEKYN
                     EYIVRRII"
     gene            16224..18509
                     /locus_tag="MJE33_002520"
     CDS             16224..18509
                     /locus_tag="MJE33_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBY9648451.1"
                     /translation="MKCLFSNVDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGVRIFIDDVELDL
                     AKCKVEDFYRELDMKNGILNRRFIVNINDKKFEVRVARFLSISVKELAVIKYSIKSLN
                     SNTKIKFVPYLDSNIKNEDSNYEEKFWNGISSSSLEYKGSILSRTKENAFNTPVFTVG
                     SMMYIKTNGDVEAISHESDYNYCENSITMDVLKNEVASIEKYVSITSTRDYKESELLR
                     KCEDILNRELSKSYDDILNEHTKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADKEVTRNLLEYRYNQLEQAK
                     DNAKKLGLKGALYPMVTFDGVECHNEWEITFEEIHRNGSIVYAIYNYTNYTGDYKYIK
                     EKGIDVIIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNINNNWYTNYIAKWCLDY
                     AVENILRLEKECNECIDRNNVTREEVEFWKIISNKMYLPYDEELQIIIQNDNFLDKEF
                     VEVKDLPKENFPLNQNWSWDKILRSCFIKQADVLQGIYYFGNRFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWNETLSFDPKLPKEWKNYSFNINYRDNIINVDVCSDLIKIENLKGR
                     PIKIIVYGKKYILEKCIKIDK"
     gene            18734..19627
                     /locus_tag="MJE33_002521"
     CDS             18734..19627
                     /locus_tag="MJE33_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBY9648452.1"
                     /translation="MKNKKFIVIILIISILIGVLVKEYSSREIKKDDINVSKYIKYAD
                     LASKNNAQVNWKYVASIVAVLNKNNLKNVKDSQIQEVSDLFVKNFSKNNKINKLSDIL
                     DELEFSNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFIDEIKTEAIQNYKDYKILP
                     SITIAQAIIESSWGKSTLAKQYSNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIY
                     DDKNESIKDHAKFLATNKRYKNNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAAR
                     LIELIQQYNLQLIDSEIQSEM"
     gene            20064..20555
                     /locus_tag="MJE33_002522"
     CDS             20064..20555
                     /locus_tag="MJE33_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648453.1"
                     /translation="MYENNKVIYNLERCEKLEKTLYLSQFNTYKNFKLASKKTVINSL
                     ETYVYNMNINNISLVDTIECISLEYEQLTGKKLILVGDIDIDAILDYAGNKRNRNSSK
                     KNTFKLKIPFSTFIQMPRKIENKDKINLKYLIQDITSSILDDNLFISVTAIISYENSS
                     KIE"
     gene            20583..21530
                     /locus_tag="MJE33_002523"
     CDS             20583..21530
                     /locus_tag="MJE33_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648454.1"
                     /translation="MRITKTTFNDVFSNDANGSIIKVEKYVDKKCASVFEILTYTIIV
                     TNTSRYKTGNIFFKDYISKYIEFINNTVKVNGIIKRGLDPQQGFYIGRIDASCKKIIS
                     FKSVVLPNSAHRIIKNSANIYYYYKCNLDKFPTRISIESNRVYTNVNKIVFKQLNISN
                     TLKAPKNLRDILKVNVNSKIFDVKPMKNLINKGSNLKLCNLIVFGCIEVEIDYSCKNR
                     SNFKTVNKSNDIKKDKYYKNDIQNNNENINDCKKQISFGDNKVKKITKTFGFSCFICS
                     PVGIIYEDMKNINIKIEHTSINELNPEELFVNTSLLLYY"
BASE COUNT         8606 a         2292 c         3823 g         6985 t
ORIGIN      
        1 acatataaaa atatattcct gaatcaagaa tgagatattt aagattcaaa atatatatta
       61 ttatacgtga ataataaaaa gtaagaaaat tttataaggg aggggtagat gtgagtagtc
      121 aagaaagcaa accaaaagga aatcttatat caaattttag agatgatatg agagacccta
      181 agaaaagagc aatatacaca tattcaagtt ctataaactt agcgtacaat tttataggtt
      241 actttgtatc aactgttaca aatatttttt taactgatgt ggcaaaactt ggtgtaatta
      301 tggcaggtgg tatgcaaatt atacaatctt tcataaaaat tgctgtagct cctataacag
      361 gtacagtatt tgacaaacaa ccattcaaaa aaggaaagta ttatccatgg cttagatatg
      421 caccagcagc gttagcatta acatatggaa tatttttcat agttccatta atgaaaataa
      481 gtccagcatt acttttacct ttgatgacag ctttattatt aatagcatct tgtgctcagc
      541 aagtagtttt cactataatt tatgctattt accctgtagt tgctaagaca cctagagacc
      601 gagttatagg ctcaactact acgaatatat ttaaagaagt tggtaaattc ttagtaggtt
      661 tttcatatcc gttattatta gttttatttg cagatgtatt aggtggagaa ggaatgggtt
      721 atttaggtac ctacttattc tttgcagcag gatgcctttt agtattttgg ttttcttcaa
      781 cagagataat aaaatctgga gcagaagaag aagttcttca aaaagaagag aagccaaaga
      841 ttaaagcaag tgaaatggca aaagctttat tcactaatgt accacttatg atggcatttt
      901 gtttagaatt tcttatatgt ataagatcta taggattagg acctttggct ccatattatt
      961 ttaaatatgt aatagaagat gaacgtggat tagcagtatt tttatcaatc atgcctttgg
     1021 taagtgtagc attcatgttt ttcgcaccag tgtttataaa aatctgtaga gagcaaaaac
     1081 tagcttcaat aatttctttt tctatatgtg cactatgcca cttattggtt gcatggactc
     1141 cttgggggaa aactacttta ggagtaacta tgttgctggc aattggtgga ggtttttcaa
     1201 atgtagtatc aataataaat cttaatttct ttgccggttc ttgtgattat gggcattgga
     1261 aatcaggaaa agatttgcct ggtttaagta tgtcactata tccagttgca atacaagtag
     1321 gagtattact tgcaactact ataagaacag ttttaatgaa ctcaatggga tatcaagctg
     1381 atatggtagt cacagaagca gttaaatcag gttttataaa tatgatttct tattctatgg
     1441 caataccttt aataatagct gttgttatag caatactata tccagtaagt gacaagaaat
     1501 taaaccaaat aagagaagag ttaaatcaaa gaaatgctta gttaaaagaa agttaagtag
     1561 atggttaatt taagctatta aagtaagtag acgcaatagc gtttatataa atgaaaatac
     1621 atatacagag ggggctttta aaaatgatag aaaaattatt gagaaatacc gtaaaagaat
     1681 tacatcaata tgttccaggt gaaccaatag aaaaagttaa ggaaaaatat ggtgtaaaag
     1741 aaataataaa attagcttca aatgaaaatc ctctaggacc atcaccaaaa gcaatagaag
     1801 ctatgattga aatgctaaaa caaggtcaac tttatccaga gccagaagct aacgaactta
     1861 gaagaaaatt agctgaaaag ctagatttaa aaccagaaaa ttttattgtt gctaatggtg
     1921 cggataacgt tatcactcta ataggtgagg cttttataaa tagaggagat gaagttatat
     1981 attgtaaccc aacatttcca tcatatagaa ctgcaactat aaaaaatgaa gggataccag
     2041 tagaagtacc tttaacagaa gattataagt atgatttaca aggtatatta gataagatta
     2101 cagataaaac aaaattaata tgtgtatgta accctaataa tccaacagga acaattgtgg
     2161 atgataaaga gttagaagaa tttttaaaga aagtccctga aaatattata actatattgg
     2221 atgaagcata tatagaattt ttaacagttc ctaattatgt ggatggactt aagtatgtaa
     2281 gggaaaatta taacgttata gtaacaagaa ctttctctaa aatatatggt cttgcagggc
     2341 ttagagtagg atatggtatt gctaaagatg agataataag aactttattt acagtaaaag
     2401 agcctttctc tgcaaatcgt gttgctataa gtggagcaac agcagcactt gatgatgaag
     2461 agtttataaa agaaacatat gaattaaata gagttggaat ggcttacttt aaagaagagt
     2521 ttacaaaaat gggatttgat gttgtagatt cacaatcaaa tttcctttat gtagatatga
     2581 aaacagatat acctaagtta tttgaagact tgaaaaaaag agggtttgta ataagaccaa
     2641 gtgctacaca tgcacgtgta agtattggaa ctatggaaga aaataggaaa tttgtagcag
     2701 ttttaaagga aatattaaat attaaaggag agccaagtat tggataatag atagaaatat
     2761 tatgaaacgg ggtgtagata tgaacttaaa aataaacgca gagagatgta aaagttgtga
     2821 atactgcgtt atatcttgta aaaaaggagc attaaaaata tcaagtaaga taaataaaga
     2881 aggttatgct catgtagaag ttgatgagag taaatgcata ctttgtggaa tttgctatca
     2941 ggtgtgtcca gataatgttt ttgaaataat taaagaagaa gcatgtcaag aggcttagaa
     3001 ggagttggga taatatgaaa aagatgtgga aggggaacca cgccattgca gaagcagccc
     3061 taagaggagg ttgtgaattt tatgcaggat atccaattac accacaaaca gaggtaatgg
     3121 aatttttatc acatagaatg tctgaacttg gaagaacttt tatacagtcc gaaaatgaga
     3181 tggctgctat atatatggta tatggagcat atgcaagtgg aatgaggtct atgacgagtt
     3241 cttcaggacc aggaatatct ttaaaacaag aagggatttc ttacttatgt gcaaatcatt
     3301 acccttgtgt aatcgtaaat gtacagagat gggggcctgg tcttggctca ttggactcag
     3361 cacagacaga ctatctaaga gatactagag ggggaggaaa tggagattat catttgatag
     3421 tttatgcacc aaacagtatt caagagactg tagatttgat gtataactca tttgatgtag
     3481 cggagaagta cagagtgcct gtcgaaattt tgacagaagc agctttaggt caaatgatgg
     3541 aaccagtaga atttccagag tttaagaaga gagaaggaga tttaggttgg acttatgatg
     3601 gaagtaatag agaccatgca aaggttgcag ataaccaaaa gccaactttt tgtatggaga
     3661 aacgtatgag aataactgaa aatgaacaac aatgggaaga ttatcaagta gaagatgcag
     3721 aatatgtgtt tgtagcgttt ggtataccat caagaacaac tatgaatgct gttagaagac
     3781 ttagagaaca aggtgaaaaa gttggaataa taagaccaat aacagtatgg cctttcccat
     3841 ataaagcatt tgaaaaggta agtaaagatg taaaaggatt tatctcagta gaatcaacag
     3901 acacaggtca attagtagaa gatgttgctt tagcctcaaa gaaagtatgt aaagaaaatg
     3961 tacctgtgta tggtttattt agtggaaatc atataccaaa aacattgcaa gtaatggaca
     4021 cttatagcaa aataaaatct ggcgaaatca aggaggtgtt ctaatatggc tacagaaaaa
     4081 ttaacaccta gcgttgtttc aataccaaac aagttttgcc caggttgtgg tcatggaata
     4141 gtaaatcgta taatagcaga ggtaatagaa gaacatggat atgaaaaaaa tcatgtatta
     4201 acattaggag ttggttgtgt ctgcaatatg aattttagct ggaatggtga taagatgcaa
     4261 actgcacatg gtagagcttc atctacagct ataggagtta aagtagcact tccagataca
     4321 ttagtaatga cttaccaagg tgatggagat gcttatgtta taggtctttc tgaaacttta
     4381 aatacagcat atagaaatca taatgtgact gtatttgtaa taaacaataa taattttgca
     4441 atgactggtg gacaaatgtc atggaccact atgccgggtc aagtcacaac aacaagtgta
     4501 aatggaagag atataaagac tactggtagt ccactgaaag ttcctgagat ggtggcaaac
     4561 ttctttgatg tagcatatgt agcaagaggt tcagttcata gcccaaagga aataattaat
     4621 ctaaaaaaat atataaaaaa tgcaatagaa gctcaattaa atggagaggg atattcctta
     4681 gttgaaatat tagcaccttg cccaactaac tggggtgtat cactagagaa atctatcaaa
     4741 tggatggaag aagaaatagt gccatattat gctcttggcg aatttaagca aagggatggt
     4801 gaatagttat ggaaaaagag ctagtatttg cagggtttgg tggtcaagga gttttaactc
     4861 ttggtctgat tgtagctgag tcagcacttg aattggggaa acaagtaact tggatgcctg
     4921 catatggtcc aactatgaga ggtggaaaag cctatagtgt agtaaagttt tctgatgaga
     4981 gtataggtgg gcctgatatg gaagaaatag atgttttagt agcaatgaat aaaccatctt
     5041 tagattatat aaatttagtt aaagaaggtg gaacagtagt tattaataca tctgcaatag
     5101 atgagaatgt aacattaaga gaagatataa gtgtagttaa aataaattgt caggaattag
     5161 ctcaaaaagt aaacaatcca aaagcagcaa atatagtagt acttggagca ttaattgcta
     5221 aaactgggtt atttgaaaaa gaattagctt taaaaactat gtgtgatttc tttgaggaaa
     5281 aaggaaaagg aaagtttaat gttcaaaatg aagcagcatt catggaaggc tataataacg
     5341 ccatttgata aattaccctt atcttaagtg atttatctca agatttaata atctttagtg
     5401 ccaagagaaa cttcatcttg agataaaatt cacttaaaat cacaactata aacatatagg
     5461 agcgtgaatt tatgagtttg gaaaaactta ttagaccaaa aagtattgct atagtaggag
     5521 tgactgataa acttggtttt ggaagaagtg cagcacttag tatagtaaaa agtaaggaaa
     5581 cagatagagt atattatgtt aatcccaaaa gagaagaatt gtttgggaga aaatgttata
     5641 agacgataca agaagttcca gaagtagtag actgtgtagt agtatgtacg cctagaaatg
     5701 tagttccatc tgtattaaaa gattctggag agttaggaac aaaggcagca gtggtatatg
     5761 ctagtggatt tgcagaggaa ggtacagagg aaggtacaga tttagaaaat caacttatag
     5821 aaatatcaaa tacatacgat atgaaaatat tgggtccaaa ctgcatgggg ttgctaaatt
     5881 gtatagataa ggttaatcta tgggcaggag gttctaaatg ggatttatat actaaaaaac
     5941 ctggtatagg aattgtaggt caaagtggat ttataactgc tgaaattgtt tcaagtgatt
     6001 attttaatat atcttatggt ttttcaacag gaaatggaaa tatagttaca ctagaagatg
     6061 cagtagattt ccttgtagat gatgattatg catcagtaat agcaatttac cttgaaggtt
     6121 taaaaaatcc tcaaaaattt atagatgctt taaaaagggc agcacaaaaa agaaagccag
     6181 taataatatt gaaatcagga aggtcagaaa aaggtgctat ttctgctgct tctcatacag
     6241 gaaatttagc tggttcgagt aaagcttttg aaagtatttt tgaaaaatat ggagtaattt
     6301 ctgtggaaaa tttagaacag tttatgtgtt tagcacaagc ttttagtgtt ttagatggta
     6361 atttacctgc aaattctaat tttgcagcaa taaacttctc aggaggagaa aatactattt
     6421 gtgcagattt agcagaagaa aatggtgtag aacttgcaga aatatcttct gaaactaaag
     6481 aagaaatgaa aaaatatctt ccaggatttg caacacctaa aaatccacta gatgctacaa
     6541 cagcattatt ccatgaaaag gatatgatag taggtttatt acataccttt gaaaaggatg
     6601 atagtgttgg atttactatg gtaggtgcaa atataagaga tgaagaaaat gaaatgcatg
     6661 aaaccctttg taaagctgta agtgaagcta gagaacaagg attgaaaaaa cctgtgtttg
     6721 cagttcctac acttgaagga aatagatatt tagattatag gactagatta gaagataatc
     6781 aagtgcctat aatgtcatca gttggaacta catttacttg ttttaataaa atggctaagt
     6841 ttatagatta tgattattca aaaagaactt tagaatttaa agctgttaag aaaagagagt
     6901 caaataatgt agtagcatta tcagaattag atagtaagat tgagatgaaa aaatatggag
     6961 taccagttcc aggacaaggt aatgcaaaaa gtattgatga attagatgaa atcttaaaat
     7021 atattaagta tcctgttgtt ttaaaaataa attcttctga aatattgcac aaatctgatg
     7081 ttggaggagt taaaatagga attaagaata gagatgaggc agtagatgca tataatgaga
     7141 ttttaactaa tgtaaaaaaa gcaaaaccag atgctaatat tgatggtata ttagttcaag
     7201 aaatggtaga aagtggtata gaaattatta ttggtataac taatgatgac caatttggac
     7261 cactgttatt agtcggttta ggaggagtat ttgtagaggt atttaaagat actacattat
     7321 atccattacc tataaatcat gatgaagcta ttatgatgct taaaaaacta aaatcattta
     7381 agcttttaaa tggctataga ggaagtgagc cttgcgatat tgatgctctt gcagatatga
     7441 tggtcaaatt aggaaaatat gcttatgaaa ataaggatga agtcaaggaa atagacttaa
     7501 atccagtgtt tgtatatcct aaaggaaaag gcgtatgtgc agtagatgcc ttaatagtta
     7561 aatataaata gtttataagt tttggtattc aaccataatt aaagcaccga ctgaaaatca
     7621 cattttgtaa gttagatagt gtgattttca gtcaccccaa atttaattta aaaatatttt
     7681 aaaatgaaag tatagtttta agtttcattg tgaaatagca ctgtataaac aataaaatac
     7741 tacgtataaa acgaataata aaaggtgttt taatgataag aatcaatctt gattttcatt
     7801 aaaacacctt ctttattata ttaaaaattt ttaaacgtta tattgtcaca ggtgggtttg
     7861 ttggagctgc atatgtacga ttagcaaatt cttggtcaac aaagaataga ccaccctctc
     7921 cagcaagttt aacacgtttt atattgtctt cacaatttgt ctcttcttct gcttgttcag
     7981 atataaacca aagtaagaat tgagaagttc taaagtctga ttcagagttt gctacagctg
     8041 ctaagttgtt tattaaagct gtaacctttt tttcatgagc taacgtacgc tcaagaatat
     8101 ccattgcaga agtaaagtct gaatctggca ttgggatagc atcaagctga acctttcctc
     8161 ctacattatg taagtatttg taaaaaaaca tagcgtggtc tgtctcttct ttatattgaa
     8221 cataaaacca attagaaaat cctttaagtc cttctgcttc aaggtaagaa gacatagaaa
     8281 gataaagata cgcagaatat aattcatggt taatctgctc attaagtaat ttttccattt
     8341 tagctgaaat catttttata cctccaaatt ttaaatgctc ttataaaata taataatatt
     8401 aaatggaaaa atattcaata aaagtgttta aatattttac atatttaata aaaatttata
     8461 ctaccattaa aaaaattata acttacaatg gctatagagc caattaatgg tgcatttgct
     8521 ccaaaatttg aatgccttac acttattttt ttatatttag aaaaacttgc tgaagcagtc
     8581 aattttttta ataatgtatt ttctataaat gtaccaggta tattaaagtc atagcctatt
     8641 attatagaac tacaatcaat caatgtaagt gtattaacta atgcatatga tacgtatgta
     8701 caaaattcat caagcaaaaa catggcaaga ctatctttat tatttgcctc atctattaac
     8761 ttgactaaag aaaaatcttt acagttaatt aatggggagt taggataaat atggctcaat
     8821 gattcaattc ttttaattag attactgaca cttgtataat aatctaaaca tccattgtta
     8881 ccacaactgc acaatggtcc actaaaatta attgatgtat gtcctatttc tccactttgt
     8941 cctaggttgc cagtatgaag tttattttct agaactagac cagctccaat accattcatt
     9001 atgtgtaggt atatataatt tgatatattt ttacctaaac catacatttt ttctgctaat
     9061 gcacccgaat tagcatcatt tatcaaaaat attggcaaat cagacatttc ttttataaaa
     9121 tctactatat ttaggtttga tatatttcca aaatctgggg gatttagtat ggtaccattt
     9181 atatcatcta ctggacctat tgaagaaata ccaattccaa ttatatctct cttgttgatt
     9241 ttcattattt tagtaaacat tctttttatt atatctttta aaacatcatt tgatagacat
     9301 ttagggtaag tttcagaaag ttgttttaca atattacctt ttaaatctga aataattacc
     9361 ttacatatat ttcttcttat taggattcca caaatacaag gcgatatatt agaaatatct
     9421 aataaaatgg gtttacgacc tgatgaatgt gcatttaaag ttgatgactc tatttcccta
     9481 atcatatttt cattaataag ttcagtaact agatttgata aagtcatttt agtaagtcct
     9541 gtagacttag ctaaatcaac tctggacata ggtgtatttg tagaaagtaa tttcaatatt
     9601 attaacttat ttttaatttt tacatctttg atgttagaac cttgtttgtt actcatcata
     9661 aatctcctta aataaagtta agtgttttat ttattttcaa gtatactatc accttataat
     9721 agagtaacat ttttttactt gaaaatatca ttaattagaa tatatcacat tatatatcat
     9781 aaaacatata aaaaaatatt agaattaaat taaaagtgaa aagtagattg atatataaat
     9841 agtaaatact taattaaaaa aagataatga tagcaataat tattgttacc aagaatctac
     9901 tacttcaagt gttaccttag gtatttaagt tgatagtggt ttgcacgtaa taaacttgat
     9961 gtaatatata ttcttaataa tattgataaa agatttactt aaaaataata tagatcatgt
    10021 aaacatataa tctatttatt gtatacaata aaatttcatt ttgtaaaaca gtgaattaaa
    10081 tatgtaatat agatatcaat ttttattaca aattgttact aaatataggt acattagaac
    10141 aaaaatctag taaattgtaa ctaaaatgta aatttttcct ttaatctata caatattatg
    10201 gaaacttttt gtaatttatg atataataac aaatatataa ttattaaatt ataataaatg
    10261 actatagcgt agatgtaata ctatgttata gtcatttttg aattaaactt gatatatcaa
    10321 gggggaagaa tatgagaaca attagaagag ttttagcatt aggtttaaca ttagctatat
    10381 ttctaataaa tgtacctaat gtagatgcat taacatcaga tacaattaaa ggtaacaata
    10441 tatatgagac ggctggatta atagcagata aaaagagtta tgatacagca attatggtaa
    10501 atatggataa ttctatagca gatggtcttt cagcaagtgg tcttgcaggt gctgttgatg
    10561 ctccaatttt gcttgcacaa aaaaataaga taccaaatga aacaaaacaa agattaaaaa
    10621 atgttaaaaa gatatacata ataggtaaag aattatcaat aagtaagtca gtagaaactg
    10681 aactaaaaaa cacaggggca caggtaacta gattaggtgg agatgacaga ataaagacta
    10741 gttatagtgt tgctaaagag gtaaatggta tcaaaaaagt tgatgaagtg atattaacta
    10801 atgcatataa aggagaagca gatacaataa gtgctgcacc tgtatcagta agagatatag
    10861 ctcctatagt acttacagat ggaaagagtg tgcctttttc aacaagcaat gtaaaaacat
    10921 atgcagtagg tgggagtata tcaatgagca ctagtttagt taataaaact aatgctaaaa
    10981 gacttggtgg ttcagataga tatgatacaa ataagaaagt tataaaagag ttttatccag
    11041 atgcatcaga attttatttg agtgatggat atgatttagt aaatgcactt acaggttcta
    11101 caattgctaa ggaaaatcca attgtattgg tgtcagaaag tagtgataag tctatattag
    11161 caggagcaga taagattact agattaggtt caataagtga cagtgtgtat aataagtgtg
    11221 tttctgctgc acaaaataat ggcgattcgt ctacaaaagg tgagtcacct atgaaaaatg
    11281 agacaagcat attaggtcag ccaacagcta gtttagaagc gtgcttaaaa tgggcaaaat
    11341 ctaaaaaagc taatgattta tttatagagt taataccaat attatatgat actgctgttc
    11401 aggaaggtgt taatcctgtt ttggcagtag ctcaatctgc aaaagaaact ggtttttgta
    11461 attttggtgg agtattagat gcatcattta agaatccttg tggacttaaa acttctgtag
    11521 gtggttctga tactgataag aatgcccatt caagatttga tacttgggaa gaaggaatat
    11581 tagctcaaat tcaacattta tgcttatatg caggacaaga tggttaccca ctttcaaatc
    11641 cagtagaccc tagacatgaa aaatctttat ttggtaaggc taaaacagtt gaaagtttat
    11701 ctaataattg ggctggaggt caatatggac aagatttagt aagaatgatg ggagaaattg
    11761 aggcaacaaa ataattgaag attatcaaat atccaataaa taaggattga ttattatgaa
    11821 aaagagtaat atttttagta tcatatttcc tacagtcata atggtgctaa tgtttttaac
    11881 ttttgctaca gatatattta atattccaga tattcaatct aaaagtattt ttgttgtggg
    11941 aatggttttg atttttccaa tctctttttt gatacaaggg ataatttgtg tattaaataa
    12001 gactaattgg attttatctc ttattgtgtc tttagtaact tatatagtat taatgtatat
    12061 atttttaaat gattctgcat atatatatgt tttactttat acagcttttt atatgattgg
    12121 acatataaca gctaaactct attacaaaat taagactttt aaaaattaaa ttgaagtata
    12181 taaaagtaac attaagagaa gtatccttaa tgattttcta aaatcagaaa taggtgcttc
    12241 tcttttttgt aaaaataaaa gtatgtaaat aaaatttaac cattataatt ttcttttaac
    12301 aaatgtatac aataaacttg ttgtataatt aagatagata aaataaaggg agggataata
    12361 tgaatttaat agataatcat attcatacta atttttcaag tgatggaaag gattccatgg
    12421 aagatacaat aaaaaaagct ataagtattg gtgttagata tttgactttt acagaccatt
    12481 tagaacatga tgaagataga ggattttgca taaattataa tgactatgtt ccagtattta
    12541 ataagtttaa agaaaaatat aaaaaggaca tagagttatt actaggtgtt gaagtgggtt
    12601 acagaaaaca tctaaaaaat gaaatagaag aaataataaa ttctaatcct tttgattttg
    12661 tattgtgctc aactcataca attgacaatg tacctgttcc atcaaaggag tattttaaag
    12721 gtcttagcaa agaagatgcg tattataaat attttaatag catacttgaa actaatcgtg
    12781 aatttggaga ttacaatata tatggtcatt tggattatat atctaggtat ggaatatatt
    12841 cagataatag agttatatac aatgacttta aagatgttat tgatgaggtc ttaaagtcaa
    12901 taatcaataa tgggagtggt atagagctaa atacttctgg ttatagatat ggactaaatg
    12961 ctatacatcc aaatgaagat attctaaaaa gatataaaga acttggagga tttatagtta
    13021 ctgtaggttc tgattctcat agggtagaag atatatgcaa agattttgat gtggcctatg
    13081 atatgcttaa gtatcttgac tttaaatatg tatctttatt taaagaaaga gaaacttatt
    13141 ttgtaaatat agaaaaagtt aaatctaaca atatagctta gagtgtgttt tgaagtaaat
    13201 taatggatag ttaaaaagtt aatagttata atattgtatc ctctagaaaa atactagagg
    13261 tttttatttt gctaaaataa ctaaatagaa ttaaatttaa atagtatagt ttattttaaa
    13321 ttgaatagtt tattgttgat atattttact attatatatt tttattgtga tataatagta
    13381 aaatatatta acctatcttt agattatata aattattata aaggagggaa attttaaaat
    13441 gtctaataaa aataaagaaa atataacttt aagtaatcaa atcaaaggaa caattcaaaa
    13501 tagtttaaca aaagaagcta tattacatat accaatgagc aattatgcct atggttatga
    13561 tagagaaaca ctacatataa gacttagaac taaaaaaaat gaagcgaaaa gagtaatttt
    13621 acgaatagga gaccaatatg tgtgggataa aggaggtgca ggaggaggaa atttaaatgc
    13681 atctggagtt ggttggtctg gtggaacaaa tatagtaatg agtaaagaag tagaaactga
    13741 attatttgac tattggatag ctaagtgtaa gcctttaaat aaacgctcac gatatggttt
    13801 tataatagaa ggtcaagagg aaaaactttt atttacagaa aaaagaataa tagagttggg
    13861 aaatgaaaat gatgaaaagg aattatgtga aataggtaat ttttttggat atccatattt
    13921 aaattatata gatataccta aagtgccaaa ttgggtgaaa gatactgttt ggtatcagat
    13981 atttccagat agatttgcaa atggtgaccc gtctataaac ccagaaggag tagataaatg
    14041 gggagctatt ccaaccagag ataattttac aggtggagat ttacaaggtg tgatagaaca
    14101 cttagattat ttgagtgatt tgggaataaa cggaatttac ttttgcccta ttacaattgg
    14161 aaaaaccaat catagatatg atacggtaga ttatatggag gtagacccta cattaggaga
    14221 taaagagact ttaaaaaaac taatagaaga agcgcataag agaaatataa gaataatgtt
    14281 ggatgctgtt tttaatcata taggatatta ttctaaacag tggcaagatg tagttcaaaa
    14341 taaggaaaat tctcgatata aaaactggtt ttatataaaa gatatgagta aaattgatac
    14401 tccaatagaa caaatagatg aaaaaaatat accttatgaa acctttgggt gtgaaaaata
    14461 tatgcctaaa cttaatacag aaaattcaga agtaataaaa tatttattgg atgtaggcaa
    14521 gtactggata caggaatttg atatagatgc gtggagatta gatgtttcaa atgaagtaga
    14581 ccatgttttt tggagaaagt ttagagaaga agtcaaaaaa gtaaaaccag acatttatat
    14641 attgggagaa atttggcatg gtagtttacc atggctaatg ggtgaccaat ttgattcagt
    14701 tatgaattac ttgatgtcag aagccatgaa aaaattcttt tgtacagatg agataaacgc
    14761 cgaagaattt aagtatatga taaatgatgt aatggtaagt tatccaatac aagttagtga
    14821 agttattttt aatttacttg gaagtcacga tactacaaga atattgactt atgctaatgg
    14881 aaatattgat agatttaaac ttgcatatat gtttatgttt gtacaaacag gatgtccttg
    14941 tatatactat ggtgatgaaa tcggaatgga aggagaatta agttcaatat ctgaaggtca
    15001 aagaaaatgt atgaaatgga atgaagaaaa atggaataag gatatatttg attttatgaa
    15061 gaagattatt agacttcgta aagaaaataa agaacttagg tctatatcaa atgaatgggt
    15121 gttagcagat aaggaaaatg gaacgattat attgaaaaaa gaatctattt ctataataat
    15181 aaataattca tctaaaaata taattttgac gttaccagat tatttagcaa ataaaaaagt
    15241 aaaagattta tatgaagaag aaatagttga tttaaaagaa gaactaatgt tagagaaata
    15301 caagttcatt attttaaaat aatacaaaaa agtatttatt ttagatttca tgttaaatta
    15361 ttatacagtt taaataagtc tttataaacg ataaaaaaat atggtctaca agtgagtgaa
    15421 ttgaaagtag attttaaatt aaagactgag ttagagtaga taattttata aataatatct
    15481 taaatacaag atagaaaata aattgattat tagtctaaaa atttcttgta gaaagaggaa
    15541 ataagaatga ttgaagcatt tatatttgat ttagatggtg taattacaga tactgcatat
    15601 tatcactata tggcttggag aaaactagcc cataaagttg ggatagatat agacacaaaa
    15661 tttaatgaat ctttaaaagg cataagtaga atggagtcat tggacaggat attagaattt
    15721 ggaaataaga aatattcatt ttcagaagaa gaaaaagtga gaatggctga agaaaaaaat
    15781 aattactatg tatcattgat agatgagata acttcaaatg atattttgcc aggtatagaa
    15841 agcttgttaa ttgatgtaaa atctaataat ataaagatag gtctatcttc tgcaagtaag
    15901 aatgcaataa atgtattaaa tcatttgggt ataagtgata aatttgattt tatagctgat
    15961 gcaggaaaat gtaaaaacaa taaaccacat ccagagatat ttcttatgtc agcgaaaggg
    16021 cttaatgtaa atccacaaaa ttgcataggg atagaggatg caagtgcagg tatagacgct
    16081 ataaactcag ctaatatgtt ttctgttggt gttggaaatt atgaaaactt aaaaaaagca
    16141 aatttagttg tagattcaac aaatcaatta aagtttgaat atatacaaga aaagtataat
    16201 gagtatatag ttaggagaat tatatgaaat gtttatttag taatgtagat gagtggaaga
    16261 ttatacaaga taagattgaa atcaaagaaa atagattagc agagtccata atgagtatag
    16321 gcaatggata tatgggtatg agaggaaact atgaggaaaa ttatagtgga gatacacaca
    16381 aggggtctta tatagctgga gtatggtttc cagataaaac tcgtgttggt tggtggaaaa
    16441 atgggtatcc agaatacttt ggtaaaattc ctaattcagt caactatata ggtgtaagaa
    16501 ttttcataga tgatgtagaa ttagatttag caaaatgtaa agtagaagat ttttatagag
    16561 aattagatat gaaaaatggt attttaaatc gtaggtttat tgtaaatata aatgataaaa
    16621 aatttgaagt tagggttgct aggtttttaa gtatatctgt taaggaactt gcagtaatta
    16681 aatacagtat aaaatcatta aattctaata ctaaaataaa atttgtacca tatttagatt
    16741 caaatataaa aaatgaagat tcaaactatg aggaaaagtt ttggaatgga atttcttcaa
    16801 gttctttaga atataaaggg agtattcttt ctcgaacaaa ggagaatgca tttaatacac
    16861 cagtattcac tgtgggttca atgatgtata ttaagacaaa tggtgatgta gaagctatat
    16921 ctcatgagag tgattataat tactgtgaaa attcgataac aatggatgtt ttaaaaaatg
    16981 aggttgcaag tatagaaaaa tatgtatcta taacttcaac tagagattat aaggaaagtg
    17041 agctattaag aaaatgtgaa gacattttaa atagagaact atctaaaagt tatgatgata
    17101 tattaaatga acatacaaag ttatggaata aaagatggga aagtgcagat gtaaaaataa
    17161 atggagataa aagtagccaa caaggtataa gatataattt attccaacta ttttcaacat
    17221 attatggaga agattcgcga ttaaatatag gtccaaaagg atttactggt gaaaagtatg
    17281 gaggagctac ttattgggat acagaagcat tttgtgttcc agtatatcta ggtgtagcag
    17341 ataaggaggt aactagaaat cttttagaat ataggtacaa tcagttagaa caggcaaagg
    17401 ataatgcaaa aaaactaggg cttaaaggtg ctttatatcc aatggtaaca tttgatggag
    17461 tagaatgtca taatgaatgg gagataacat ttgaggaaat ccaccgaaat ggaagtatag
    17521 tatatgcaat atataactat acaaattata caggtgacta taaatatata aaagaaaaag
    17581 gaatagatgt aataatagaa atagctagat tttgggcaag tagagttcat ttgtctacaa
    17641 aaaaagattt gtatatgata catggagtaa caggtccaaa cgagtatgaa aacaatatca
    17701 ataataattg gtatactaat tatatagcta aatggtgttt agattatgct gtagaaaata
    17761 tcttacgatt agagaaagag tgtaatgaat gtattgacag aaataatgtg actagggaag
    17821 aagttgagtt ttggaaaata atatcaaata aaatgtatct gccgtatgat gaggaactac
    17881 aaataataat tcaaaatgat aattttttag ataaagaatt tgtagaagta aaggatttac
    17941 caaaagaaaa ttttcctctt aatcaaaatt ggtcttggga taagattctt aggtcgtgtt
    18001 ttataaaaca agcagatgta ttacagggaa tttactattt tggaaataga tttactaaag
    18061 aagaaaaaaa gagaaatttt gacttctatg aaaaatatac tgtacatgaa tcttctttat
    18121 cttctagtat ttattctatt attgcctcag aaatagataa cctagaaaag gcttatgaat
    18181 tatattcaag aacagctagg cttgatttag ataattacaa taatgataca aatgatggac
    18241 tgcacataac ttcaatgtct ggttcatggc tttccattgt acatggattt gcaggcatga
    18301 gaacatggaa tgaaactcta tcttttgacc ctaagttgcc aaaagagtgg aaaaattatt
    18361 ctttcaatat aaattataga gataatatta ttaatgtaga tgtatgtagt gatttaatta
    18421 aaatagaaaa tttaaaaggt agaccaataa aaattattgt atatgggaaa aaatatatat
    18481 tagaaaaatg tataaaaatt gataaataga tttaataata tttttattat ataaatagtt
    18541 aaaataagct atctaattat ttatttattt taactatttt gtgtgataga aaactatata
    18601 ttataacaat agtagtgctt ttaattaaat atatttcata aatcttcata aaatcttcaa
    18661 aatttcgtaa agaatatgtc acaaaagtaa ggtatattat attcagcaga tagtataagg
    18721 gaggacaata tttatgaaga ataaaaaatt cattgttata atactgataa tatcaatttt
    18781 aataggtgta ttagtgaagg aatattcatc tagagagata aaaaaggatg atataaatgt
    18841 cagtaagtat ataaaatatg ctgatttagc aagtaaaaat aatgcacaag ttaactggaa
    18901 gtatgtagct tccattgttg ctgtgttaaa taaaaataac ttaaaaaacg ttaaggattc
    18961 acagatacaa gaagtatcag atttatttgt taagaatttt tctaaaaata ataaaataaa
    19021 taagttaagt gatattttgg atgagttaga atttagtaat agacaaaaaa gattggttga
    19081 taattatata gacaatctta aggattatgg tataaaacca gaaagattga agtctgatac
    19141 aaaatatatg aagtttattg atgaaattaa aacagaagca atacaaaatt ataaggatta
    19201 taaaatactt ccatctataa ctattgcaca agctataata gaatctagct ggggcaagtc
    19261 aactttggca aaacaatata gcaatctatt tggtataaag gctgacgctt attggaaagg
    19321 taagtcagtg actttagaaa cgaaagaaca tttagataca attatagatg ataaatttag
    19381 aatatatgat gataaaaatg aatctataaa agaccatgca aaatttttag ccacaaataa
    19441 aaggtataaa aacaatggtg tatttgatgc aaaaacgtat atatatcaag ctaaagcatt
    19501 agaaaaggca ggttatagta ctgcgaagga tgaaaatggc aatagtatat atgcagctcg
    19561 tctaatagaa ttaattcaac agtataatct tcaattaata gacagtgaaa ttcaaagtga
    19621 aatgtaactt tgattaaatt tttattgata aggctatgat ttttaaaatc atagccttat
    19681 tttgacatgc ttttacttgt aattattaaa tcgatatcat tatctttcaa aatttacttt
    19741 atactgtaaa tcaaattatt taagttaaaa cttggaatta tttataaaag gattatttac
    19801 tttatctaaa ttctgcactt aaagaacatc ttttccctta agactgaccg ttagtctata
    19861 aaaattataa ttacttataa aaaagtgaat atatatataa agttttcaga aagactttta
    19921 cttgtaaatt gaaatcaatg aagtaaaaaa ggattctttc ttaaaagtaa ataacaacta
    19981 gatagtgaat aataaaaaat ggtaatttga aataattttt atgaatagta ttaatatata
    20041 gatacaggga gattgatgcg attatgtatg aaaataataa ggtaatatac aaccttgaaa
    20101 gatgtgaaaa actagaaaag actctttatt tatctcaatt taatacatat aaaaatttta
    20161 aattagcttc taaaaaaact gtaataaatt cattagagac atatgtttac aatatgaata
    20221 ttaacaatat aagtcttgtt gatactatag aatgtatatc attagaatat gaacaactta
    20281 caggaaagaa gttgatttta gttggtgata tagatattga tgcaatatta gattatgcag
    20341 gaaataaaag aaatagaaat tctagtaaaa aaaatacatt taaattaaaa atacccttta
    20401 gtacatttat acaaatgcct agaaagatag aaaataaaga taaaattaat ttaaagtatc
    20461 ttatacaaga tattacttca tctattttag atgataattt atttattagt gttactgcaa
    20521 ttataagtta tgaaaatagt tccaagatag agtaatcttt aaattataga aaggtggatg
    20581 caatgagaat aacgaaaact acttttaatg atgtattttc aaatgatgca aatggaagta
    20641 ttataaaagt agaaaaatat gttgataaaa aatgtgcatc tgtatttgag attttgacct
    20701 acactattat tgttacaaat acaagtaggt acaaaactgg aaatatattt ttcaaagatt
    20761 atatttctaa atatatagag tttataaata atactgtaaa agttaatggc attataaaac
    20821 gtggattaga cccacaacaa ggtttttaca tagggagaat agatgcaagt tgtaaaaaaa
    20881 taatatcttt taagagtgta gttttaccaa attctgcaca taggattata aagaatagtg
    20941 ctaatatata ctattattat aagtgtaatc tagataaatt cccaacaaga atatcaatag
    21001 aaagtaatag agtttatact aatgtaaata aaattgtatt caaacaattg aatataagta
    21061 atactttaaa agctcctaaa aatctaagag atatattaaa agtaaatgta aatagtaaaa
    21121 tatttgatgt taaacctatg aaaaatctta taaacaaagg tagtaatttg aaattatgta
    21181 acttgatagt ttttgggtgt atagaagttg aaatagacta tagttgtaaa aataggagta
    21241 attttaaaac agtaaataag tctaatgaca ttaaaaaaga taagtattat aaaaatgata
    21301 tacaaaataa caatgaaaat ataaacgatt gtaaaaagca aataagtttt ggtgataata
    21361 aggtaaaaaa aataacaaaa acttttggat tttcttgttt catttgttct ccagttggaa
    21421 ttatttatga agatatgaaa aatataaata taaaaattga acatacatca ataaatgaat
    21481 taaatccaga agaattattt gtaaatacat cacttttatt gtattattaa gtattttaaa
    21541 gctaaaactt aaacttatta aaatagcatt tggttaaagg aaaatagata ttgatatatt
    21601 tatcatatat ataaatgatt aattaaacaa ttataaataa taagtatagt ataatattta
    21661 aaaaatatag atttatgata aaataagaca aatatgttaa aaaaat
//
LOCUS       DAGSRM010000064        21687 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.064, whole genome shotgun sequence.
ACCESSION   DAGSRM010000064 DAGSRM010000000
VERSION     DAGSRM010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21687
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.064"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            127..1578
                     /locus_tag="MJE33_002524"
     CDS             127..1578
                     /locus_tag="MJE33_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBY9648455.1"
                     /translation="MDFFNGILTSINNAVVYVNNYLWSYILIIMLVVIGIYFTIKTNF
                     VQFRYFIEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGG
                     PGAVFWMWLIALIGSASSFVESTLAQIYKVKDGQAFRGGPAYYMEQGLNKKWMGILFS
                     ILITICYGFVFNAVQANTVSLAFNNAFGISKMAMGIGLGILTAIVIFGGVHRVAKVSE
                     IIVPVFAGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRG
                     LFSNEAGMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSS
                     GATGIELAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLN
                     VFRVFVVGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQK
                     KSGIKDPVFIADEIEGLEKVSEWHRELSEEHLG"
     gene            complement(1797..2258)
                     /locus_tag="MJE33_002525"
     CDS             complement(1797..2258)
                     /locus_tag="MJE33_002525"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBY9648456.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVSP
                     ENIEIVWVPGAFEIPLVAQKMAKSGKYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDSELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLN"
     gene            complement(2363..3556)
                     /locus_tag="MJE33_002526"
     CDS             complement(2363..3556)
                     /locus_tag="MJE33_002526"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HBY9648457.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDESRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGADTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDTYSNEEHIALTYGNINIENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRIPINEEINDFNKLYLKTKKDKMGH
                     LLDII"
     gene            complement(3579..4244)
                     /locus_tag="MJE33_002527"
     CDS             complement(3579..4244)
                     /locus_tag="MJE33_002527"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HBY9648458.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKINTILLTKNVGDFVNLESDVIGKYVNNFMANNYKELNSNSNNHKSNIDKDFLFK
                     NGF"
     gene            complement(4278..5390)
                     /gene="ribD"
                     /locus_tag="MJE33_002528"
     CDS             complement(4278..5390)
                     /gene="ribD"
                     /locus_tag="MJE33_002528"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HBY9648459.1"
                     /translation="MNFVNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCVIVKASNI
                     IGKGYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKR
                     VVISTLDPNPLVCGNGVKKLKDNNIDVTVGILEDEARDLNEAFFYHIKNKRPLCIVKS
                     AVSLDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQE
                     KVSHPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESIKLSMLKENNVETIISPSK
                     NNLVDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNKLVDKVKLFLSPKIIGGKDAPT
                     FVSGEGINHLSDSTQLTINNVTLIDGDILIESDVLN"
     regulatory      complement(5522..5643)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            5832..8315
                     /gene="recQ"
                     /locus_tag="MJE33_002529"
     CDS             5832..8315
                     /gene="recQ"
                     /locus_tag="MJE33_002529"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBY9648460.1"
                     /translation="MNKKLLEILSKYYGYTSFRKGQESIINSILSEKDVLAIMPTGGG
                     KSICYQIPALILDGMTIVISPLISLMKDQVDALKTMGISAAFINSSLSSKEFNEILNN
                     IRKNNYKILYIAPERLDAQEFIELINNNNVSQVAIDEAHCVSQWGHDFRLSYRRISDF
                     IKNLPKRPIVTAFTATASEEVRTDIINLLCLENPDYYITGFDRENLSINIVKSSSKNK
                     YILDYIQNHKNESGIIYVATRKEVENIYNGLSKRNISVSKYHAGLSKNERSKNQEDFI
                     NDNVDIMVATNAFGMGIDKPNIRWVIHYNMPQSIENYYQEIGRAGRDGEKSECVLLFS
                     PGDVHIQKYLIDIGVENPERKLFQHKKLQYMIDLVYSNSCYRKTILSYFGENYIDNCN
                     NCSNCLVEGEIVDKTIDAQKVISCVARMKRSYGVTTIVDVLRGSKNKKILQLGLNTLS
                     TYNIMRDYSSEDLKNFVNTLVSHGFLDLVETLGNGNRGSFPTIRLNEMSMKVLKGEVK
                     VEFKEIVMTKSLEVEDELYNALRELRHSIASEERIAPYMVFGDGTLRAMSSSYPINKE
                     EMLDISGVGEIKYQKYGRDFEAVIKEYVEKNNINKKIDEKNVDSKNVNSDFLEVNTDK
                     VLYEKLLVIREEYATKEKLPAYMILANKALKEISGRYPLDEEQLKDISGIGGVKFEKY
                     GASILAEVNKYVKENNINVIWEKKGKRKLILDGESRKNNEIALDLLNQGKDIQSISQE
                     IEVSLSTIIGYVHDYVKDGHSISFELGLENFYNEDKKKMILEVCEKVGYDNLNNIKRK
                     LPDSIKYENIRAVILDSYLENLKNNIIAE"
     regulatory      8404..8515
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8657..9958
                     /gene="thiC"
                     /locus_tag="MJE33_002530"
     CDS             8657..9958
                     /gene="thiC"
                     /locus_tag="MJE33_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBY9648461.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            10012..10206
                     /gene="thiS"
                     /locus_tag="MJE33_002531"
     CDS             10012..10206
                     /gene="thiS"
                     /locus_tag="MJE33_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HBY9648462.1"
                     /translation="MKVNGKEIEFEKDLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            10199..11002
                     /gene="thiF"
                     /locus_tag="MJE33_002532"
     CDS             10199..11002
                     /gene="thiF"
                     /locus_tag="MJE33_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HBY9648463.1"
                     /translation="MVKIKIFVNELLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKT
                     DMTLCENDKLTLIQKGVKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DIFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGEGLMAPRVAICANHMANLATQILIDM"
     gene            11065..11835
                     /locus_tag="MJE33_002533"
     CDS             11065..11835
                     /locus_tag="MJE33_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HBY9648464.1"
                     /translation="MDKLVLGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMA
                     RAFKLAVEGGREAYIAKTGNVSEFANASSPLTGFLGNL"
     gene            11852..12955
                     /gene="thiH"
                     /locus_tag="MJE33_002534"
     CDS             11852..12955
                     /gene="thiH"
                     /locus_tag="MJE33_002534"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HBY9648465.1"
                     /translation="MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNL
                     LSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEVISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYKFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            12956..13645
                     /locus_tag="MJE33_002535"
     CDS             12956..13645
                     /locus_tag="MJE33_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBY9648466.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELRK
                     LYMKIKTKINCIDFQEQISDESLKTNINQKEFRNKFKVNFIINSNIEFFEKVDCQGIH
                     LPFKLFLNLIENKYNFNENKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSHIYETK
                     CKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAIMSTIMRSKDIK
                     KTISNYNEKLN"
     gene            13833..15227
                     /gene="yfcC"
                     /locus_tag="MJE33_002536"
     CDS             13833..15227
                     /gene="yfcC"
                     /locus_tag="MJE33_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBY9648467.1"
                     /translation="MTDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFAAIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
     gene            15744..17111
                     /locus_tag="MJE33_002537"
     CDS             15744..17111
                     /locus_tag="MJE33_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBY9648468.1"
                     /translation="MGLKEFDFIEESIDMLRTMSPTLETISDEIEEYFENILDEKNQE
                     YINVTSRIKSESSLREKIIRNRYLKKYGEASNLIHNVSDLIGLRIECRFIEDENKIYR
                     LLRRYFNKTDDKINYYNKENKNIKLKLSERQPNKQKNGFEIYKIDGVFAYLDREVKFE
                     LQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSIKNNLTMIDNQLLSIYKNFH
                     SGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFVVDFKKPCETILNYSFNKQDIS
                     DEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTFDDKFCKDLGNHFRSRLNTEFP
                     WNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENREQLLCQFEEDSKLIIEDIYDC
                     IFNSISEIDSVEILYNYNLEKINFTSSEIINCICREYECYGEYLEEKEKLMNTFKEKI
                     IQIFE"
     gene            17136..17744
                     /locus_tag="MJE33_002538"
     CDS             17136..17744
                     /locus_tag="MJE33_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HBY9648469.1"
                     /translation="MRHFSNIYELSKIILEEAICIEKTGVILAGGKNSRMGRDKAFLE
                     LHDKLFIEIAIEAFKNFDELIIISNNEELYSKYDIKVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNNQIVERIANKMDDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYKKLTKV"
     gene            17792..18163
                     /locus_tag="MJE33_002539"
     CDS             17792..18163
                     /locus_tag="MJE33_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBY9648470.1"
                     /translation="MNLLKCDSWAVILNNSDKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDVILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
     gene            18342..18680
                     /locus_tag="MJE33_002540"
     CDS             18342..18680
                     /locus_tag="MJE33_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBY9648471.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAEAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNSILEK"
     gene            19146..19649
                     /locus_tag="MJE33_002541"
     CDS             19146..19649
                     /locus_tag="MJE33_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HBY9648472.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGVYKVTEYVLI
                     KDDKEMLKENIIRLCNSNKIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEVLRGEATE
                     CATKKSE"
     gene            19696..20655
                     /gene="moaA"
                     /locus_tag="MJE33_002542"
     CDS             19696..20655
                     /gene="moaA"
                     /locus_tag="MJE33_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HBY9648473.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVKFDKNYINENLS
                     FEDYKFIIKAMAEQGITKIRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRIFELEKCGLNKVNISLDSLKEYKYKSITRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLIENKCSDTNRRMYEIGG"
     gene            20659..21129
                     /gene="moaC"
                     /locus_tag="MJE33_002543"
     CDS             20659..21129
                     /gene="moaC"
                     /locus_tag="MJE33_002543"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HBY9648474.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDVDDSMSAIKIYATCK
                     SLGKTGVEMEALNACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKFE"
     gene            21165..21596
                     /locus_tag="MJE33_002544"
     CDS             21165..21596
                     /locus_tag="MJE33_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HBY9648475.1"
                     /translation="MAKVVSINISEKKGVIKVPVQCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENVTTEGIEVFSLPVGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVKVLKEGIINVNDSIDVI"
BASE COUNT         8361 a         2341 c         3748 g         7237 t
ORIGIN      
        1 aaaccttgta atttgatttt ataaatatta taattaaatt aatgaatttt taacaaggaa
       61 ttattcattt ttttgatttt aagatttatt gagaaaacaa aactaaatag gggggtaatt
      121 aatataatgg attttttcaa tggtatttta acgagtatta acaatgcagt agtatatgta
      181 aacaattact tatggtcgta tatcttaatt ataatgttag ttgtaattgg tatttacttt
      241 actattaaaa caaattttgt acaattcaga tattttatag aaatgtttag acttctgggt
      301 gatggtacag cgaataaaaa tgcaaaaaag gaaggaaaaa tctcttcatt tcaagcattt
      361 tgtataagta catcttcaag agttggaact ggaaatatag ctggaatagc aattgcagtg
      421 gtagcaggag gacctggtgc tgtattttgg atgtggctta tagcattaat tggttctgca
      481 tcaagctttg ttgagagtac attagctcag atttacaaag tgaaagatgg tcaagctttt
      541 agaggtggac cagcgtatta tatggaacaa ggtctaaata aaaaatggat gggtatttta
      601 ttctcaatac ttataactat atgttatggt tttgtattta atgcagttca agcaaatact
      661 gtttcacttg catttaataa tgcatttgga ataagtaaaa tggctatggg aataggtctt
      721 ggaattttaa ctgcaatagt tatttttggt ggagtgcata gagttgcaaa agtttctgaa
      781 ataatagttc ctgtatttgc aggattatat atattagttg ctttggctat agttgtaatg
      841 aatataacag agataccaag tgttatagca ttgatagttg aaagtgcatt tgattttaaa
      901 ggaatggcga ttggtacatt tatgggagtt gtaatgactg gagtgaagag aggtctattt
      961 tctaatgaag caggtatggg tagtgctcct aatgctgctg caacagcaca tgtaactcat
     1021 cctgtaaaac aaggtttaat tcaatcttta ggtgttttta cagatactat aataatatgt
     1081 agttgtacag cattcatggt attattatat tcaggttata gtagtagtgg agcaactgga
     1141 attgaattag ctcaagaagc tttaactcat catataggac cagttggaaa tatatttata
     1201 gcagtttgta tattcttgtt tgcatttagt tctatagttg gtaattacta ttatggtgaa
     1261 tcaaatatgg agtttatgag tggaagtaag actaaactta atgtatttag agtttttgtt
     1321 gtaggtatgg ttttatttgg ttcacttact caagtggatg tagtttggaa tcttgcagac
     1381 ttatttatgg cattgatggc gataataaac ttaatagcaa tagcactttt aggaaaatat
     1441 gcatttattg ctttaaaaga ttatacaagt caaaaaaaat caggaataaa agaccctgtg
     1501 tttattgcag atgaaataga aggcctagaa aaggtatcag agtggcatag ggaattatct
     1561 gaggaacatt taggataaaa aatagtataa aattataaaa ccattttgaa agactagagt
     1621 atgtatatac tttatatgaa tataatattg aattaattta tattactgga atttttaatt
     1681 ccagtaatat ttttttatta tataatttag ctttattaaa agtttttatt attaaaaata
     1741 gcttatttat cgtaaagtga atgatattgg gttttaatta ttatgaatgt tagtatttaa
     1801 tttaaagact taaataaatt tgccatttct agcgcattca tagcacaatc ataaccttta
     1861 tttccagctt ttgtacctgc tctttcaata gcttgttcaa tattttctgt agttacgatt
     1921 ccaaatataa caggtaattc actgtctaaa gatactttgg ctatcccttt agagacttca
     1981 ctacatacat aatcataatg agaagtagcc cctcttataa cacagcctaa acagattata
     2041 gcatcatact taccactttt agccattttt tgagcaacaa gaggaatctc aaaagctcca
     2101 ggtacccaaa ctatttctat attttctgga gaaacgtcat gtcttaaaag acagtcttca
     2161 gctcctgata aaagttttga tgttataaat tcattaaaac gagaatttat tatacctatt
     2221 tttaaatctt ttccaataag ttttccttca tatatcattt ttacttcctc caattttatt
     2281 tatgttatag atttatattt ataaatatct ttaatttaat tataaaaaac tacttagtag
     2341 aaaaatatca tcatataatc atttatataa tatctaaaag atgtcccatt ttatcttttt
     2401 tagtttttaa atatagctta ttaaagtcat taatttcttc attaataggg attctatttt
     2461 cgatttttat tccatatttc tctaattgat ttattttatc tgggttatta cttaaaagat
     2521 ttatactttt tatgtttaaa ttttttaata tttgagcagc catatagaaa tctctcatat
     2581 cttcttcaaa tccaagcatt aaatttgctt caacagtatc gtaaccttct tcttgaagtt
     2641 tataagcctt aattttattt aaaagaccaa tacctctacc ttcttgcttc atataaatta
     2701 aaactccact tccattttcc acaattttct tcatagaaga ttctagctgt aatccacaat
     2761 cacattttag agagtggaac acatctcctg ttaaacattc agaatgaagt ctaactagag
     2821 tattttctat atttatattt ccataagtaa gggcaatgtg ttcttcattt gaataagtat
     2881 ctctatatcc tatgatttca aaatttccat attttgtcgg aaggaaagca ctagatactt
     2941 tttctatagt agtttcattt attttgcggt attctataag gtcttttata gtgatgattt
     3001 ttaaattatg cttttctttg aaaatcatta aatcatcaac tcttgccatt gtaccatcat
     3061 ctttcattat ttcacagata agaccaatct ctttaaaacc tgcaagtcta gcaaggtcta
     3121 ctgaggcttc tgtatgtcca tccctgacaa gaaccccacc agtttttgct attaaaggaa
     3181 agatgtgacc aggcgttttg aaatctttac ttgtagaatt ttcatcacag aataatttta
     3241 cagtttcagc tctttcatat gcagatatac cagttagagt atctgctcca tctatagtaa
     3301 cagtaaaggc tgtttgaaat gtatctgtat tttctctaac cataagaggt atattaagac
     3361 ttttaaattt tttttcagtg gcaggtagac aaattagacc tctaccatat gtagccataa
     3421 aatttattga ttcaggagta gccatttcag ctgccatcaa taaatctcct tcattctctc
     3481 tactttcatc atcaaccacg attaccattt tcccattttt tatatcttca atagcttctt
     3541 caatagtatt aaacatgtaa ttcactcctt tattattttt aaaaaccatt tttaaataaa
     3601 aaatctttat ctatatttga tttatggtta ttactattag aatttaattc cttgtagtta
     3661 ttagccataa aattattaac atactttcca attacatcgc tttctaaatt aacgaaatct
     3721 ccaacattct ttgtcaagag aatagtgtta attttagtat gaggtataat agaaacctta
     3781 aatatttcat catctaaata tgatatagtt aaacttacac catcaatagc aacagaacct
     3841 ttgagtatca ttgaggataa aagtttttta tcaggtcgta tacttactaa aactgcattt
     3901 tcatcttttt caaatttgca gatagttcct ttaccatcaa catgacctgt tactatatga
     3961 ccaccaaatc ttgaagaaag actcatagct ctttcaagat ttaccttgtc atttgcattt
     4021 aataaaccta aatttgtgga acgaattgtc tccatcataa catcggctgt aaattcattt
     4081 tttccgaggt ttgaaacagt tagacaaact ccatttacgg ctatactatc tccaagattt
     4141 gtgccatcta atattttatt tgatagtatg gttacttttc cactcttacc attagcagta
     4201 atttttctaa gtattccaac ttcttctact atgccagtaa acatcctaac ctcctatatg
     4261 aatgtattaa caaaaattta atttaaaaca tctgattcta taagaatatc gccatctatc
     4321 aaagttacat tattgattgt taactgtgtt gaatctgaca aatgatttat tccttctcca
     4381 gaaacgaatg taggtgcatc tttaccacct ataatcttgg gtgatagaaa taatttgacc
     4441 ttatcaacta acttatttct aaagagactg tcattgagag tagcaccacc ttcaactaga
     4501 acagaatcaa tgtttaactc tcctaattta tacattacaa actccaaatc tactaagttg
     4561 ttttttgaag gagaaattat tgtttcaaca ttattttctt ttaacataga taattttata
     4621 ctttctttgc aacatgtgaa aactatagtt ttactggttt tatctttaac caaattacta
     4681 ttaagtggca actttaggtg tgtatctatt actattcttg taggatgaga cactttttct
     4741 tgattgagcc tacaagttag taaaggatta tcgttcaaaa ctgtatttat gccaaccatt
     4801 atactttggt atttatttct gtatttatga gttagatacc ttgatgattc attagatatc
     4861 cattttgatt ctagtgattt tgtagcaatt ttaccatcta gacttactgc tgattttaca
     4921 atacaaagag gtcttttgtt ttttatatga taaaaaaatg cctcatttaa atctcttgct
     4981 tcatcttcta atattcccac agtaacatca atattgttat cttttaattt ttttactcca
     5041 ttaccacaaa caagtggatt tgggtctaat gtagatatta caactctctt aatcttattt
     5101 tgtataattt tatctacaca aggaggcgtt ttcccataat gagaacatgg ttctaagttt
     5161 acataaagag tagagtcttt tagactttgt ttagcactgt ttatagcatt tacttcagca
     5221 tgattagagc caaatttctc atgatatcct ttgcctatta tattactagc tttaacaatt
     5281 acacacccaa ctaatggatt tggatttaca aagccttctc catttttagc caattcaata
     5341 gcttttttca tataataaat atctttttct ttttgattca caaaattcac aatatgtaca
     5401 cctcctgttt aattggataa tttttaaaca aaaaaccctg agaattaact cagggtttag
     5461 aataatctac aataaatgtt taataaatgt agaacaaaat tgctctacaa aaacactcat
     5521 taaatatctt cttccatcca gactttactg tcggctttgg aatttaacca aatcaaccaa
     5581 gttttacctt ggctcgcggg ctataccgcc gattgggaat ttcaccctac cccgaagatt
     5641 aagtattaaa tttatattta gaatgtatca taaattattt tactttgcaa actatttttt
     5701 taaaaaatat atgttaatat ataatataga atgtctataa actttgaaat accacgattg
     5761 tgaatataaa aataaaaatt tgaacataat caggaaagaa gtctaattaa tttaaattag
     5821 gagagtaata tatgaataaa aaacttttag aaatactatc aaaatactat ggatatacaa
     5881 gttttagaaa aggtcaagaa tctataataa actctatctt aagcgaaaaa gatgtgttag
     5941 ctattatgcc aacaggaggg ggaaaatcaa tatgttatca aattccagct ttgattttag
     6001 atggaatgac aatagtaatt tctccactta tatcacttat gaaagaccaa gtagatgcct
     6061 taaagactat gggaattagt gctgctttta taaatagttc attaagtagc aaagaattta
     6121 atgaaatatt aaataatatt agaaaaaata attataaaat actctatatt gctccagaga
     6181 gattagatgc acaagagttt attgagctaa taaataataa caatgtaagt caagttgcga
     6241 tagatgaagc acattgtgta tctcaatggg ggcatgattt taggttgagt tatagaagga
     6301 tttctgattt tataaaaaat ttgcctaaaa gacctatagt tacagcattt actgcaacag
     6361 cttcagaaga ggtaagaact gatataataa atttactgtg tttagaaaat ccagattatt
     6421 atataactgg atttgataga gagaatttat caataaatat agtaaaatca tctagtaaaa
     6481 ataagtatat tttagattat attcaaaatc ataaaaatga gtctggaatt atatatgttg
     6541 caactagaaa ggaagttgaa aatatatata atggtttatc taagagaaat atatctgtgt
     6601 caaaatatca tgcaggttta tctaaaaatg aaagaagtaa aaatcaagaa gattttataa
     6661 atgataatgt ggatataatg gtagcaacaa atgcatttgg aatgggtatt gataaaccta
     6721 atataagatg ggttatacat tacaatatgc ctcaaagtat agaaaactac tatcaagaaa
     6781 taggtagagc tggtagggat ggagaaaaaa gtgagtgtgt acttttattt tctccaggag
     6841 atgtacatat acaaaaatat ttaattgata ttggagttga aaatcctgaa agaaaattat
     6901 ttcagcataa gaagcttcaa tacatgattg acttagttta cagtaattca tgctatagaa
     6961 agacaatatt gagttatttt ggagaaaatt atatagacaa ttgcaataat tgtagcaatt
     7021 gtttagtaga gggtgaaata gtagataaaa ctatagatgc acaaaaagtc atttcatgtg
     7081 tagctagaat gaaaagaagt tatggtgtta ctactattgt agatgtgctt agaggttcta
     7141 agaataaaaa gattttacag cttggattaa atactttgtc tacatataac attatgagag
     7201 attattctag tgaagactta aagaattttg taaatacatt ggtttctcat ggttttttag
     7261 atttagttga gactctaggc aatggaaata gaggtagttt tcctactata agattaaatg
     7321 agatgtctat gaaagtttta aaaggtgaag tgaaagtaga gtttaaagaa attgttatga
     7381 caaaatcatt ggaagtagag gatgagttat ataatgcttt aagagaactt agacattcaa
     7441 ttgcaagtga agaaaggata gctccatata tggtatttgg agatggtaca cttagagcca
     7501 tgtctagtag ttatccaatt aataaagaag aaatgctaga tatctctgga gtaggagaaa
     7561 ttaaatatca aaaatatggt agagattttg aagcagtaat taaagaatat gtcgaaaaaa
     7621 ataatataaa taaaaaaata gatgaaaaaa atgtcgattc aaagaacgtt aatagtgatt
     7681 tcttagaggt taatacagat aaagttttat atgaaaaact gttagtcatt agagaagaat
     7741 atgcaacaaa ggaaaaatta cctgcatata tgattttagc caataaggct ttgaaagaaa
     7801 taagtggaag atatccactt gatgaagaac aattaaaaga tatttctggc ataggtggag
     7861 taaaatttga aaaatatgga gctagtattt tggcagaggt taataaatat gtaaaagaaa
     7921 ataatattaa tgttatttgg gagaaaaagg gcaaaaggaa gttaatatta gatggagaaa
     7981 gtagaaaaaa taatgaaata gcattagatt tgctaaatca aggaaaagat atacaaagta
     8041 tcagtcaaga aatagaagtt tcactgtcaa ccattattgg atatgtgcat gactatgtaa
     8101 aggatggaca tagtatatca tttgaattag gactagaaaa tttttataat gaagataaaa
     8161 agaagatgat tttagaagtt tgcgaaaaag ttggctatga taatttaaat aacataaaaa
     8221 gaaaacttcc agattcaata aagtatgaaa atataagagc tgtgatactt gactcttatc
     8281 ttgaaaactt aaaaaataat attatagcag agtaattttt aacttaataa aaaataaaat
     8341 ttgtattatt gtgttgaact aataattttg ctgtgttata ctgaatttgt aaaaataatg
     8401 aaaaattaga gctaggggcg ctagaattgg ctagctgaga gataaagccg taacttacaa
     8461 accctaataa cctgatcaag ataatgcttg cgaagggaag ctttttaata taattttatt
     8521 atataaatag gcaaattatt caatgttagg agaaatttgt ctaagtgtat atacgattat
     8581 aagagtgctt tccaaacgag gaaaagcact ttttttattt tcaaaaacta taactttaat
     8641 cttggaggat tgtaatatga actatacaac acaaatggat gctgctagaa aaggcataat
     8701 aactaaagaa atggaaatcg tttctcaaaa agaacaggta gatgtaaatg aattaagaga
     8761 attaatagca aatggacaag ttgttatacc agctaataaa aatcataaat ctttaagtgc
     8821 agaaggtgta ggtaaaaatt taagaacaaa aataaatgta aatctaggaa tttcaagaga
     8881 ttgtaaggat atagaaaaag aacttgaaaa agttagagta gcaatagaca tgaaagctga
     8941 agctataatg gatttaagta attatggcaa aactagagaa tttagagaaa aggttgttga
     9001 aatgtcacct gcaatgatag gttcagtacc tatgtatgat gcagtaggat atttggaaaa
     9061 agaattaaaa gatataacag aagaagagtt tttaaatgta ataagacaac atgctattga
     9121 tggagtagac tttattacta tacatgcagg tcttacaaga agtgtttgcc aaaaaataaa
     9181 aaatcatgag agattgactc atatagtatc tcgtggaggt tctttattgt ttgcatggat
     9241 ggaattaaat aataaggaaa atcctattta tactaatttt gataaaatat tggatatatg
     9301 tgaagaatat gatgttacat tgagtttagg agatgcttgt agaccaggtt gtataaaaga
     9361 ttctactgat ggtgttcaaa ttcaagaact tgtagtatta ggtgaactta ctaaaagagc
     9421 atgggaaaga aatgtacaag taatgataga aggtcctgga cacatggcta ttgatgaaat
     9481 agaagcaaat gtagtattag aaaagagatt atgccatgga gcaccttttt atgttcttgg
     9541 accattggtt acagatatag caccaggtta tgaccatata acaagtgcaa taggaggagc
     9601 tttagcatgt gccaaaggag tagatttttt atgttatgta actccagcag aacatttaag
     9661 attgcctaat ttagatgata tgaaagaggg tataatagct gcaaagatag ctgctcatgc
     9721 aggagatata gctaaaaatg ttaaaggtgc tcgtgaatgg gataataaga tgagtaaagc
     9781 tagagctgat ttagactggt gtgagatgtt tagacttgca atagaccctg aaaaagctaa
     9841 gagatacaga gatgaatcaa ctcctactca tgaggatagt tgtactatgt gtggaaaaat
     9901 gtgctcaatg agaacagtta agaaaatatt aaacaatgag gaacttaatc taatataatt
     9961 tttggctagt atgactggaa taaaaataag ctgaaaagag gaggtgattt agtgaaggtt
    10021 aatgggaagg aaattgaatt tgaaaaagat ttgactgtta tagatttatt aaataaatat
    10081 aatttaaaaa gtgatagagt tgtagttgaa gtgaatttag agataattga agaatctaac
    10141 tacaatacat atgtattaaa agacgaggac attgtagagc ttattagctt tattggaggt
    10201 ggttaaaata aaaatatttg taaatgaatt attaacagat gttgaggaaa atacaactgc
    10261 ttataaagtt agagatagct ataacaataa atgtgatgta ttggtgttaa atggatatcc
    10321 aattaaaaca gatatgaccc tttgtgaaaa tgataaatta acacttatac aaaaaggagt
    10381 taagccatca cttgatgaat tggaaagctt gttgattgct agacatactc caaatataca
    10441 taataaatta aaaaaaggga aagttgctat attgggattg gggggacttg gttccaatat
    10501 agctatctct ttagctagaa taggagtggg agaactttta cttatagatt atgatgttgt
    10561 agaaccatct aatttaaata gacagcagta ttttatagat gatataggaa aactaaaaac
    10621 tgatgccatg atagaaaata taaaaaaaat aaatccattt ataaaattaa ataaacgaga
    10681 tattttttta aacaagaaca atatggatac tattaaggat tcagatttaa taattgaggc
    10741 ctttgatgat gcttcatgta aagcacaggt atgtaattat gtattaataa atttaaaaga
    10801 taaatattta atagcatcat ctggtatggc aggttactat gattcaaata taataactac
    10861 taagaagata aaagacaggt tttatatatg tggggatttt gtgaatgaag ctaaatttgg
    10921 tgaagggctt atggctccaa gggttgccat ctgcgcaaat catatggcaa atttggcaac
    10981 gcaaatactt atagatatgt aaatattaaa attagatata aaaaagattg aatattaaaa
    11041 aaatttaacg tggagggaaa taatatggat aaattggtac ttggtggaca tgaatttaat
    11101 agcagattat tggttggaac aggtaaatat ggttcaaata atatattgcc tgaggtaata
    11161 aaagaaagtg gaagtgaaat aataacaatg gcacttagaa gagtggattt agacaataaa
    11221 caagaaaaca tattaacgta tattccaaaa gagatgacaa tacttccaaa tacatcagga
    11281 gctactaatg cagaagaagc tgtaagaata gctagaatat ctagaaaaat gggatgtgga
    11341 gattttataa aaatagaagt aatatcagat actagatatt tacttcctga taatgaagaa
    11401 actataaaag ctacaaagat acttgctgat gaggggttta tagttcttcc atatatgaca
    11461 ccagaccttt atgctggaag aagacttata gaagctaatg cagcggcagt tatgccactt
    11521 ggagctccaa ttggttcaaa tagaggtctt caaatgaaag aaatgataag aataatgata
    11581 gatgagttgg atataccaat aatagtagat gcaggtattg gaaaaccatc tcaagctatg
    11641 gaggctatgg aaatgggagc tgatgcagta cttgtcaaca ctgctatagc ttcagcagga
    11701 gaccctgtac aaatggctag agcatttaaa ttagctgtag aaggtggaag agaagcatat
    11761 attgcaaaaa caggaaatgt gtcagaattt gcaaatgcat cttctccttt aacaggtttt
    11821 ttaggcaatt tatagaaagt ggtgattggt gatgagtttt tatgatgtaa tagaaaaata
    11881 tagggatttt gattttgatg gatatttaaa taatgttact gataatgatg ttttaagaag
    11941 tttatcaaaa gacaagcttg aagattttga tatattaaat ttgctttcta aaactgcagt
    12001 aaaacattta gaagatatgg cacaaaaagc acataaatta agtgttcaat actttggtaa
    12061 aacagtatgt ttatatacac caatgtatat agcaaattat tgtgttaatc aatgtgttta
    12121 ttgtagctat aatataaaga gtggaataaa aagaaaaaaa ttaactatgg atgaaattag
    12181 agaagaggga gaagtaattt caaaagaagg tttcaaacat ctacttgttt tgactggaga
    12241 aagtagtttt cactcaagtg tagaatatat aggtgaggct atagaaatat taagagagaa
    12301 atttccctct ataggtatag aagtttatcc aatggaagta gaagaatata agtacattgt
    12361 agataaaggt gttgaaggat taacagtata tcaagaaact tatgacgagg aaatatataa
    12421 gagagtacat ataaaaggcc caaaatcaaa ttataaattt agactagatg ctcctgaaag
    12481 aggggcaaaa gcaggtatga ggactttatc aataggtgca cttttagggc taaatgattt
    12541 tagaaaagaa acttttttta caatattgca tgggaaatac ttaaaaacga aatatcctca
    12601 tattgagctt tcatattcaa caccaagaat gagacctttt aaagggtgtt ttgaggaatt
    12661 ggtagatata agtgatactg atttagttca agctatggta tgtatgagat tatttgaccc
    12721 tcatgcagct ataaatatat ccactagaga aaatttagaa atgagaagtc atataatacc
    12781 tttaggagta actaaattga gtgcaggtgt atctactgat gtaggaggtc attctcaaga
    12841 tgaacatgat actgctcaat ttaagataaa tgatgaaagt actgttaaag atgtggagaa
    12901 aatgctaaac tcaattggtt atcagcatgt gtttaaagat tgggaaagat tttaaatgta
    12961 tctgataaca aacagaaaat tatgtagtga ggaacgatat ttagaagtta ttaaagagtc
    13021 tatattgagt ggtgtagaaa atataataat aagagaaaag gatttagaat atcaagagct
    13081 aagaaaatta tatatgaaaa taaaaactaa aatcaattgt atagattttc aagagcaaat
    13141 atcagatgaa agtttgaaaa ctaatataaa tcaaaaagag tttagaaata aattcaaggt
    13201 taattttata ataaatagta acattgaatt ttttgaaaaa gtggattgtc aaggtatcca
    13261 tcttcctttt aaattattct tgaatttaat tgaaaataag tataatttta atgaaaataa
    13321 aatactagga ttatctttac ataaagtaga ggaagtagat tatttagaaa aattaataag
    13381 aaatcaaaat ataaaaatag attacataac attatcacat atatatgaaa ctaaatgtaa
    13441 agaaggatta aatccaaaag gaattgagct tttaaaagaa gctaaaaaga ttactgatat
    13501 taaaataatt gcacttgggg gaattttacc atctaatgtc aaggaaacat taaaatactg
    13561 tgatgatttt gctataatgt caactataat gaggtctaaa gatattaaaa aaaccatatc
    13621 aaattataat gaaaagttga attagtaaat atagtatatt gaatttttaa cttaaataaa
    13681 aataaaaaag ttatctttta aattttaaaa ggtagctttt ttattttata tctttgctaa
    13741 taaaaaattg taaaaaggga aaactttagt cagagagatt aaacaatatc ctagaaagat
    13801 ttaaaattta gtaaagtatg gaggacatta taatgactga cagtaaaaag aaacgattta
    13861 ttataccaca cacttttaca ataatatttt tactaatagt cttgatggca atattgactt
    13921 ggatagtacc aagtggtgaa tttagtagag aagatgtaga aggtagaatg gtagttgtac
    13981 cagggactta tcaaagtgta gaaagtaatc ctcaaggatt tgcagatgtt tttaaagcac
    14041 caatagaagg ctttatcgat tcagctgaag ttgtaggttt tgttttaata gttggaggtg
    14101 cttttggtat tgtaaatagg actggagcta tagaagcagg aatagcttcg gttatctcta
    14161 agtttaaggg aaaagaactt cttataattc caatatcaat gattttattt ggacttggag
    14221 gtacaacatt tgggatggca gaagagacat taccatttta tatgatattt gttccactca
    14281 tgacatcctt gggatatgat tctctaacag caatagcaat tgtatttata ggagcagcag
    14341 caggagcagc cgcttcaaca ataaatccat tttcagttgg tattgcacag gcactagctc
    14401 aacttgctcc tggttcagga gtaacttaca gagtagttat ttttatagca tatatactaa
    14461 taagtatagc atttgttatg tactatgcta agaaggtaaa ggaaaatcct gaaagttcaa
    14521 tagtatatga aatagataaa aataataaat caatacttaa tcaaacttca acagaaataa
    14581 aagaatttac ttttaaagaa gtatctgttc ttgctatatt tggaataggg atggcaataa
    14641 tgatttgggg tgttttaagt caaggatggt acattccaga aatatcaatg tgttttgctg
    14701 caataggtgt gctttctgga ttggttggta gattatctca aattgaaatc tcagaaagtt
    14761 ttatagatgg agcaaaagat ttagcatctg cagctcttgc tatagctttg gctcgtggaa
    14821 tcgtaataat tgctcaaaat ggatttataa tagatactat attaaattcc gcagcagcct
    14881 ttctaggtgg gcttccaaag gttatatttg tatatttatc atttttcttg gaagctgcat
    14941 tggcattttt aattccatca tcttcaggtc ttgcttcact tacagtacct gtacttgctc
    15001 ctcttggtga tttggttaat atatcggctc aagtgatagt aaccgcttat caatttggtg
    15061 tgggtcttac aaactttata actccaactt caggagtact gatgggagca cttgcagtag
    15121 cccatattcc attttcaaaa tgggtaagat ttataatgcc tatcttgttg attttgacag
    15181 ctgtatctct agtcttttta acaataggta tttatatagg gttttaatta agtgtagttt
    15241 tatttaagat atttagtaat ggtataaaat gataatatgt tttcaataaa ttgccactaa
    15301 tttaattagt ggtaatttgt tttttgtatt taaaaataaa tgtatttact tgtattttga
    15361 attaatatct acttagacat tgttttaatt gttatatttt tatttaatta agcattgatt
    15421 tatcattttt tatatttcat ataacaatgc aaaaaataat ttttatatag taaatgacaa
    15481 aagtttacaa taaatgtata ttttcttact cattccttaa aattactcat ttagtttaat
    15541 aactctcatt tcatatgtaa agttggtaaa aaaattaaaa ttaaaagtaa aaaactaaat
    15601 aaattaatgt taatttgaaa tatatatgaa ggtttttgga gaatacttac ttatgatagg
    15661 aaaaaatgtt aaaataatag tgtaatcaaa tataaaggac tgaaataatt aacagttatt
    15721 aaatattagg agtgtaaaaa tttatgggat taaaagaatt tgattttatt gaagagtcta
    15781 ttgatatgtt gaggacaatg tcaccaacat tagaaactat atcagatgaa atagaagaat
    15841 attttgaaaa tatcttggat gaaaagaatc aagaatatat aaatgtaacc tctagaataa
    15901 aatcagaatc aagtttaaga gaaaagataa ttagaaatag atatttaaaa aaatatggag
    15961 aagcaagtaa tcttattcat aatgtttctg acttaatagg cctaagaatt gagtgtagat
    16021 ttattgagga tgaaaacaaa atatatagac ttttgagaag atattttaat aaaacagatg
    16081 acaaaataaa ctattataat aaagagaata agaatattaa attaaaacta tctgagaggc
    16141 aacccaataa gcaaaaaaat ggatttgaaa tatataaaat agatggagtt tttgcgtatt
    16201 tagatagaga agtaaaattt gaacttcaaa ttaaatctct tgtaaatgtg ttttggagtg
    16261 aaatagaaca taaaataatt tataaaaaca atacatattt acttgctgat aaatttataa
    16321 aggatatgat ggactctata aagaataatc ttactatgat agataatcag cttttaagta
    16381 tttataaaaa ctttcattca gggaagtctt ttaatatgaa agtaagtaaa aaagaaattg
    16441 aaaaattatt tgcgaaacta gtctatgacg ctttttctga aaagatgaat aaaagtatag
    16501 gttttgttgt agattttaaa aaaccatgtg agactatatt gaactattct tttaataaac
    16561 aagatataag tgatgaagtt ttaggtaatt ttatgcttga tgagtttgca agattaaatg
    16621 aaattgtaaa caaggatata gattttaatg agcaaataga atttgaaaga gaaccaacat
    16681 ttgatgataa gttttgtaaa gacctaggaa atcattttag aagtagatta aacacagaat
    16741 ttccatggaa cctatttttt agaatattat ttgaaataga gccatataac aataccaaag
    16801 actttgaaaa ctttgtagag tttataaaag aaaatgtgct actagaagaa aatagggagc
    16861 aattattatg ccaatttgaa gaggatagta agcttataat tgaagatata tatgattgta
    16921 tatttaatag tataagtgag attgatagtg ttgaaatttt atataactat aatcttgaaa
    16981 aaataaactt tacatcaagt gaaatcataa attgtatatg tagagaatat gagtgttatg
    17041 gtgaatactt agaagaaaaa gaaaagctta tgaatacatt taaagaaaag attattcaaa
    17101 tctttgagta ataggtcaat gctgaagtgg cgctaatgcg ccatttttca aatatatatg
    17161 aattaagtaa aattattttg gaggaggcga tttgtataga gaaaacaggt gtaattttgg
    17221 cagggggaaa aaattctaga atgggtagag ataaagcctt tttagagtta catgataagc
    17281 tttttattga aattgcaata gaagcattta aaaactttga tgagttgata ataatatcta
    17341 acaatgaaga attatattct aaatacgata ttaaagttta taatgatata gtaaaagatg
    17401 ttggtccaat tggaggaata tatactgctc ttgaatatgc aaagtatgat atagttacaa
    17461 ttgcatgtga tatgccatat ctaaataatc aaatagtcga aagaatagct aataaaatgg
    17521 atgataaaag tgtgatttca gtgacaaatg gaaagttaca accattgtgt tctggatata
    17581 aaaagagtat aataaataaa gtatctttat gtataaggga aaatgattta aagctcagaa
    17641 gctttataga taaaatagat aaaagttata tatactttga tgaagaagat ttatttttaa
    17701 atgtaaacac tgttgatgag tataaaaaat taactaaagt ttaattagct atggttgtaa
    17761 aatagagtag gatttatatg gaggagagtt tatgaattta cttaaatgcg attcttgggc
    17821 agtaatatta aataatagcg ataaagaaag taaagcatat aaaatattag atgaacttaa
    17881 aaggaatatg tacaaagttg ttgctataga tgaagagaaa aaacctattg aaggaataga
    17941 tgtgtatgaa tgtttaaaag atgttccaca caatattgat gttgtagcta ttattgataa
    18001 acaatctaag atggatgtga ttttagaaga agtagagctt ttggatatac aaaatatgtg
    18061 gtttgaaaaa ggaagcttta gtgaaatgat gattaaaaag actaaagatt taaaattaaa
    18121 tatagaatat aatttaagtt tatatgatga gttgactaga taaaattata tttcttaata
    18181 ataaaaatat aattagttaa taaaacatat tattttatag tatagattgg ttaataaaag
    18241 tacagattgg ttaatgaaac attaaattac aatatttgtt aaaaaaattg tgagttacat
    18301 gatataatgt attaaatagg atactaagga gttgatatat tatgttaata gtaactacag
    18361 aaaaagtaga aggtaaaaag atatcaaagg ttttaggatt agtgagagga agtacaataa
    18421 gagcaaaaca tgttggaaaa gatataggag caagttttaa gaatcttgtt ggaggagaac
    18481 ttactggata taatgaaatg ctcactgaag caagacaaat tgccataggt agaatggttg
    18541 aagatgcaga agctaaaggt gcaaacgcag taatagcatt tagactgtct tcagcttcag
    18601 ttatgcaagg agcagcagaa atgcttgctt atggaacagc agttgtttta gaagatgata
    18661 atagtattct tgaaaaataa aatataaata gttaaaaaga ggcatctttt aaaaatttta
    18721 tggatgcctt tttttatttg aaaaaactaa tttgagaaga aattttaact attatgacaa
    18781 taaaattagg gtgatattat ataaataagt aaaattatat tatataatgt atggtataga
    18841 ctttaaaaat tttatttgta gagaaaaata tggacaatgt tataataatt atagaaatta
    18901 aaagtactta cattcattat gtatgtgaca tgtataatta gttttatatt taatacagtt
    18961 ttgaagtaat atcttcattt tgagaacgtt ggaataataa aaaatgtatt attataggtg
    19021 atataaataa aaaataaata taatatgtta tattgctgat agaaaagaga ataaaagaaa
    19081 ataggtttta ttaaaagaga tttttattta taggacttaa tcaatgtata gaggggtgag
    19141 aacatatgtt taatgttgca attataacac ttagtgataa aggatatgaa ggaaaaagag
    19201 aagatataac aggaaaaaag ttaactgaat ttgttgaaaa tacaggagtt tataaagtta
    19261 ctgaatatgt acttataaaa gatgataagg aaatgctaaa ggaaaatatt ataagacttt
    19321 gtaatagtaa taaaatagat ttaattctta caaatggagg tactggtttt tcaaaaagag
    19381 atataacccc agaagcaact aaggaagtgt tggaaaaaga aataccaggt ctgagcgaat
    19441 atatgagaat gaaatccaca gaaattacta aaaaggccat actaagtagg ggtgtgagtg
    19501 gaataagaaa taattctata ataataaatt tacctggaag tcctaaaggt gcagttgaaa
    19561 atctaagttt tataattgat gttttagacc atggcataga agttttaaga ggagaagcta
    19621 cagaatgtgc cactaaaaaa agtgaatagt atataatcta gatagataaa aaatagacaa
    19681 agaggtagat aaagtatgat tgataagtat ggaagaaaaa tagactacct tcgtatttca
    19741 cttacagata agtgtaattt aaggtgtgtc tattgtatgc caccagatgt aaaatttgat
    19801 aaaaattata taaatgaaaa tctaagcttt gaagactaca agtttattat aaaggctatg
    19861 gctgaacaag gtataacaaa aattagattt actggaggag aacctctttt atatcctaaa
    19921 ttaaatgagc ttataaaatt tacaaaagaa gaatgtggaa taaataacat aggaatgacc
    19981 actaatgcta ttggtctttc tgatagaatt tttgagcttg aaaaatgtgg cttaaataaa
    20041 gttaatataa gtttagattc tttgaaggaa tataagtata aatccataac aagaggagga
    20101 aacttaaaag atgtcttaaa atcgattgaa tcttgtttaa atttaggtat aaaagtaaag
    20161 ttaaattgcg ttgctataag tgggtttaat gatgatgagc ttaaagattt tatgttttta
    20221 acagttaaat caccaattga tgtaagattt atagagctta tgccattagg tgaggctaat
    20281 agagtttata agaagggtta ttttaatgta aaagaaatga ttgaaaaaat atatggttta
    20341 tataaagtca aaaatgatga aaaaagtaca gcagaatatt atatgtttaa aggagctaaa
    20401 ggaagaatag gaattataac tccattaagc tgttcatttt gtaacacttg taatcgtatt
    20461 agacttactt ctagtggtgc aataaaatta tgtctacatt caaaagaaga aatagatata
    20521 aaaccatttt tacataaacc attgttattt agagaatcta tgaaagatat aataaaacaa
    20581 aaaccagaaa aacatcattt aatagaaaat aaatgtagtg atacaaatag gcgtatgtat
    20641 gaaattggag gataagtaat gttaacacat ataaatgaaa aagggtatgc caagatggtg
    20701 gacataagtg aaaaagatga tactaaaaga acggccgtag ctacagctac aattactctt
    20761 agtaaggaag cattagaaaa agtaaaaaat ggtcagataa aaaaaggaga cgtattgtct
    20821 gtagctcaag ttgcaggtat aatgggagct aaaaatacat cttcaaacat accaatgtgc
    20881 catcagatta aattagttgg atgtgattta gaatttgatg tagatgattc tatgtctgct
    20941 attaagatat acgcaacctg taaatctctt gggaaaactg gagtagaaat ggaagcttta
    21001 aatgcatgtt ctttagcagc acttacaatt tatgatatgt gtaaagcagt tgataaaaaa
    21061 atggttataa gtaatatcca tcttgtggag aagactggtg gaaaatcagg agattttaag
    21121 ttcgagtaaa ttaataatat attgggttaa aatttggagg aaatatggcg aaggtagtgt
    21181 caataaatat aagtgagaaa aagggtgtaa ttaaagttcc tgtacaatgt gcagagctaa
    21241 aaataaatca tgggatagtt ggagatgcac atgcaggaaa ctggcatcgt cagataagct
    21301 tacttggaaa agaaagcata gataaaatga aaatgaaagg ctttgaccaa ttaaaatatg
    21361 gagattttgc tgaaaatgta actacagaag ggatagaagt tttttccctg cctgttggta
    21421 caaagttaag aatagggcaa tgtgaagttg aagtaactca aatagggaaa aaatgtcaca
    21481 atggatgtga aattaagaaa ttgacaggtg actgtgtgat gccacgagaa ggaatttttg
    21541 taaaggttct aaaagaagga attataaatg tgaatgattc aatagatgta atctaatgaa
    21601 gtcactatta aaatataatt ataagtaaat actattaaga tttaaatata tattttaaga
    21661 gagaattaat tttaataaaa tatttta
//
LOCUS       DAGSRM010000065        21619 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.065, whole genome shotgun sequence.
ACCESSION   DAGSRM010000065 DAGSRM010000000
VERSION     DAGSRM010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21619
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.065"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(178..819)
                     /locus_tag="MJE33_002545"
     CDS             complement(178..819)
                     /locus_tag="MJE33_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HBY9648476.1"
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQKLLKEISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDE
                     SSSWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYRELLDIPEGYSVPFGIAVGIPDGNLPNAREVVYKVSRV"
     gene            complement(1062..1865)
                     /locus_tag="MJE33_002546"
     CDS             complement(1062..1865)
                     /locus_tag="MJE33_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBY9648477.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEESSKGKNEVMKEVLENKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGIESIAKVYVTCTKHPDGVEMARDIHQSMKDLIASFAQDPSIIPGASKGNDDFRKY
                     LLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYSPTKSDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
     gene            complement(2021..3196)
                     /gene="thiI"
                     /locus_tag="MJE33_002547"
     CDS             complement(2021..3196)
                     /gene="thiI"
                     /locus_tag="MJE33_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBY9648478.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEEATILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSILPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            complement(3242..4393)
                     /locus_tag="MJE33_002548"
     CDS             complement(3242..4393)
                     /locus_tag="MJE33_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBY9648479.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     AIKEVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFRPSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDAVISKVNNLKNILKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
     gene            complement(5041..5763)
                     /locus_tag="MJE33_002549"
     CDS             complement(5041..5763)
                     /locus_tag="MJE33_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBY9648480.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
     gene            complement(5822..6499)
                     /locus_tag="MJE33_002550"
     CDS             complement(5822..6499)
                     /locus_tag="MJE33_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBY9648481.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            complement(6527..7342)
                     /locus_tag="MJE33_002551"
     CDS             complement(6527..7342)
                     /locus_tag="MJE33_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBY9648482.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDNGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKDVTEGMAAAIKKSDNNKDFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     misc_binding    complement(7568..7778)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8455..10521)
                     /locus_tag="MJE33_002552"
     CDS             complement(8455..10521)
                     /locus_tag="MJE33_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBY9648483.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLNNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLICSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVHTANRLIDKNKNRYEKIIKCSQGDGGSVTYISPHDSEEEAL
                     YIAREIIDEIQKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWA
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDEKDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQTEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKKFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     gene            complement(10598..13627)
                     /locus_tag="MJE33_002553"
     CDS             complement(10598..13627)
                     /locus_tag="MJE33_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBY9648484.1"
                     /translation="MENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKKLN
                     DNSFDILNERVINRKNYIQNEMLQRWSNTSETEATINSSVKKVLNEKNALIEDVGNNE
                     EINKDIIKSISNDLIYLLRKNSVTGAFIILSDKNINDKSIDSKGEISRTGLYIRDLDP
                     KFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQDGDDFFYKPFRAAID
                     NPNVSSSNLGYWGSKFLLTNAGDRLMTYTVPLIYEDGTVYGVLGIDLSVNYLRELLPY
                     SEINGNKKGNYILGVDRNNDMAFENVISNGYSLKEKFWDGLHSNLEQKESYKNIYKIK
                     NKDNECGNGNIYASVQYLDLYNSNTPFENERWALLGIIEEKSLLSFSKRVEKLVIISI
                     AISLLIGLIGIYIASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIELL
                     SANVADSSSKLSQIIEMVDMPIGAFELYKNAENVFCTKEFFSVFTTEIKFEEYGYMEK
                     NKFLKKLRGLDKYLQESYETENTYIYKFESKDKKLKWIRMNIFEDELKVLGVVVDVTK
                     EFIEKRKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKIAAFIMWDLDNLKYIND
                     TYGHDAGDEYIRCAADILKKFISWNGIVSRLSGDEFYVFIYGYESKDSIRDIINSVKK
                     QMNNTLLKLHDGTEFKIRASAGISWYPDDSTNYDELMRYADFAMYQIKNTIKGEVSEF
                     DINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPKIKTFSSP
                     IDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLSKVDFEEI
                     EDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNGDAVLLYI
                     MPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVIAEGVETKGEMETLINLGV
                     DYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            complement(13680..15140)
                     /locus_tag="MJE33_002554"
     CDS             complement(13680..15140)
                     /locus_tag="MJE33_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBY9648485.1"
                     /translation="MNRCKFAKLIITSMIVLLLTGCSLKKEDNYKLDPENPTSIQIWH
                     YYNGQQKMAFDKLVKEFNDTVGAEKGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELNEYIDEYVEEGRINSKDEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIDEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFKGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLDVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDSNKINELV
                     KSGTSKKDAVAQFNNDKNFKTWFESFKAELENAIKK"
     gene            complement(15232..15606)
                     /locus_tag="MJE33_002555"
     CDS             complement(15232..15606)
                     /locus_tag="MJE33_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648486.1"
                     /translation="MGNKNKNTKYKDKTRERYNKKVENLNKEKLNKVNKQSKKVDKID
                     DIINKSSIVLCVLLVVFSLLYLFKLVQLNIIMILLVITVAIQQGLVSFGAFRNKNYVK
                     ALFSIFVIVCFLIAFKYVVNPV"
     gene            complement(15782..16522)
                     /locus_tag="MJE33_002556"
     CDS             complement(15782..16522)
                     /locus_tag="MJE33_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBY9648487.1"
                     /translation="MLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAEKY
                     KQLSQSLNSAFGGSSGVIEGGNSKIEPVVEPGSNDLDTLQNAKFKKVGEEIQKYLNEN
                     GMANSVSLRVQDRGLVISLKDTILFDTGKAIVKDNSRDKIIQIGKMLNEMNSYMRVEG
                     HTDNMSIKNSEFKSNWDLSVMRATNVVQLLIDNAGIAPDKLSAVGYGEFRPIAENSSE
                     EGRSKNRRVDIVLVDSKYDNVENVSKDK"
     gene            complement(16543..17331)
                     /locus_tag="MJE33_002557"
     CDS             complement(16543..17331)
                     /locus_tag="MJE33_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HBY9648488.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHVGALFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLAFKNKKPNNIENIIYFKEIATKARKEGLLSIDKEISEND
                     ELDPFIRKGLELMVDGVDAVTIRNILESQIQITSKRHKEGIAIFDSAGGYAPTLGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKAINQQEISE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
     gene            complement(17419..17509)
                     /locus_tag="MJE33_002558"
     tRNA            complement(17419..17509)
                     /locus_tag="MJE33_002558"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17473..17475),aa:Ser,seq:gct)
     gene            17768..18532
                     /locus_tag="MJE33_002559"
     CDS             17768..18532
                     /locus_tag="MJE33_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBY9648489.1"
                     /translation="MLPQERYEKILSILKSDNIIKINDIVQMFDISIETARRDLAHME
                     SIGLLKRVYGGAIPVIASSVELNYVKRKKINSNDKKQIAIKCSEFINTNDTIFMDTGT
                     TVLEVSKNLKDKKNLTVITNSILVANELMNTNIKVYVLGGYLRETEGSFSGPLTLSAI
                     EQFNVDKAILGAGGITLENGISDYHLEEALVRKKMIERSRKSIVVVEPPKFGINSFAS
                     VAPIEHIDTIITSASIDHNIIIEFSQKGIPLVIADN"
     gene            complement(18813..19928)
                     /locus_tag="MJE33_002560"
     CDS             complement(18813..19928)
                     /locus_tag="MJE33_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBY9648490.1"
                     /translation="MPFDKIILYIMAVGVLLGALDRIIGNKFGLGEQFEEGFNSMGPL
                     ALGMVGIVCLAPVISDVLGPIIIPIFNLCGADPSMFATILANDMGGYPLAMELAHNKE
                     AGMLAGLVVSSMLGCTLVFSIPVGLGLIEYEDRPFFAKGLLIGLITIPFGGIIGGLIA
                     GFNLSMVIINMIPVIILAVILAVGLKFNSTLMINGSLIFGKFISILITIGLGAAAFQE
                     ITGIVLIKGMTPIREGIQVIGSIAIVLLGTFPILHLLVKVLNKPLTIVGGKLGMDATS
                     AAGLVFTLANSIPVYKMMKDMSPKGKVVNTAWLVCATAALGDHLGFTAGVVPEMITPV
                     VIGKLVGGVLAIALAILMTKDLTLEEEQSIKMKKDAV"
     gene            complement(19969..21495)
                     /gene="glpK"
                     /locus_tag="MJE33_002561"
     CDS             complement(19969..21495)
                     /gene="glpK"
                     /locus_tag="MJE33_002561"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBY9648491.1"
                     /translation="MKYVLAIDQGTTGTRAVLINEKSEFVFSDYMEHTQIYPQSGYVE
                     HDPIEIWNNTKTVSNNVLQQAFENGINESDIKGIGIDNQGETVMLWDKNTGKPLYNAI
                     VWQCRRTYDYVENLKNIPGLENKIIDKTGLIIDPYFSGTKIKWVIDNVKGVKEKIKNG
                     EVLAGTLDSWLIWKMTGGKSFLTDVSTAARTMLFNIRDMDWDRELLDIIGIPRSILAD
                     IMPTSGDFGTTDKEAFCGVSIKITASMVDQPAALFGNGCFEPGMVKNTYGTGCFLYMN
                     IGEELNIAGNGILTTVAWKIGDKVTYAYDGGVYIAGAAIQWLRDGIGVIKNYSETDDM
                     ANSISSTGGVYFVPAFAGIAAPYWDQYARGTMVGITGSTKKEHIVRATLESVALQVKD
                     VVDSMNLASGKKIKMLRVDGGITKNNFTMQFQSDILGIPIEIAENPETTVLGVGYMAG
                     LSCGVWNSIDEIRSEFTISKKYIPNMTNQKREEILLGWNQAVKRAMNWENDTKKVCAN
                     "
BASE COUNT         6853 a         3996 c         2190 g         8580 t
ORIGIN      
        1 acaaaaataa aaaagaataa gataataaat aaaatactta tcaccttatt ctttatttaa
       61 ttattttaca acgaaaaatc taaactactt tttaaatagc ttcctcttcc atcaatttat
      121 ttgtatatta ctggtttaat tcttcaagta actgattggt tcttgcaata ctttatccta
      181 aactctggaa actttgtaaa ctacctctct agcatttggt aaatttccat ctggaatacc
      241 tacagcaata ccaaatggta cagaataccc ttctggtata tcaagtagtt ctctgtattt
      301 tttatttgta tcaaacttca tctttattga ctctgattct tttacatctt ctttatccaa
      361 tacactcaca agaccttcaa tatccccttg ttcaacaggt gtaaataaac ctctaaccat
      421 tcctattata caacttccaa gccctagtgc ttgtgcagct gttgttatat tccctgtaag
      481 tattcctata tcatatggag cccatgaact actttcatca taagaaataa ataaaacggt
      541 tggagcacca taaaataact taaagttttt atttatctca acattactca cctcatgaat
      601 cctagataaa gtatcatcac taatctcttt taaaagtttc tggtcttgca caacagttat
      661 gtgccaaggc tgtttattac atcctgaagg tgctgcaaat gcacagtgta ataatgtgtt
      721 tacttgctca tcactaattt gttctgtcgt aaattttcgt atacttctac gactttgtat
      781 taaatttatt gtttggttgt cttgaaaatt attattcata tctattcccc tttttatgta
      841 tttcttagta caaatactaa atcttcagaa aattaatctt ataattaaat ttctttacaa
      901 tattattata attattttta gcttgtatgt gaagacttaa aaatataaac acttttaata
      961 tatctcaatt atatatataa atgaaaatct ggtattcgtc atctatagaa actgctcttt
     1021 tctatagttt aatcaatacc agattaataa aacatgatat attaatttaa aggaccccat
     1081 ccctctttag aatgaatctc tgtttttaca gacttaacac tatctgtgtc aacatcccat
     1141 gacctacctg caacagaaat accattttta cctttatacc atctaccttc atcataatcg
     1201 taaactaggg aagtatacca attaccccca gtcttattat ttgcaaatac aactacagta
     1261 gcatcagatt tagtaggact atatgcatat ggttgtatat atagtgtatc cttacttaat
     1321 ccatattttg ccttaaattc atctggaatt gtaggccacc catttttata tttattgtct
     1381 aataaatatt ttctaaaatc atcattaccc tttgaagctc ctggtattat agaagggtct
     1441 tgtgcaaatg atgcaatcaa atctttcata ctttgatgta tatccctagc catttcaaca
     1501 ccgtctggat gttttgtaca agtaacatac acttttgcaa tgctttctat tccagttatt
     1561 ccatcataac catcatcaaa cgttgctgaa tatattccac ctgacttaca ctttggttct
     1621 gtttcaaagt acttaccatc tttgttttct aatacctctt tcatgacttc attcttgcct
     1681 ttacttgatt cctcagccat tgcaataaca atttgagtct ttatattttc tctattagaa
     1741 agacatgtaa ctgctttact tttttctata tttctaaaca atgctggaac agctactata
     1801 actaaaatgc ctattataga tataactact aacaattcaa ttagtgtaaa acccttttta
     1861 ttcataataa tcattcccat taaataaaat tatagtactt atttttaaat actacatatt
     1921 aataaattgt ctacataaat cctttcaaca gaactactaa tatacttaca gaactactaa
     1981 tatactcaat attcaattta gtaattaaat aacttgtatg ctaaaactca atatcctcta
     2041 cctctatttt gtcaatagca tcctgtacta atttttcaat gtcaagtgct gtttcagatt
     2101 tttctatttt ctctaatcta ggtttagtga cgggctttct aggagaaaat acactacaac
     2161 aatcttcttc aggcaatata gatgtttcaa aagtccctat ttttttggct atatcaacta
     2221 tatctgattt atccattgct ataagtggtc taaacactgg taagtctaca acggcattag
     2281 tacaagtaag tccttgtata gtttgagatg caacctgccc aatactttca cctgtaatta
     2341 aagcatcaca atgtcttttt tcagaaagtc tctgtgctat cctcatcata aatcttcttg
     2401 aaagaatagt agcttcttct tcattacaat tctcgccaat tgctttttgt atttctaaga
     2461 tattaacttt atgaagcctt actctaccac agtattttgc cagtatttta gctaaatcct
     2521 taactttttc ctgtgacctt tcacttgtaa atggataact atgaaagtga actgcctcta
     2581 cttccatacc tctttttgca accatccatg tagctactgg gctatctata cctcctgata
     2641 aaagagacat agctctacca ttcgttccaa gtggcaatcc accatatcca ggtatagtat
     2701 cactatagac catagtatga aattctctgt attcgcactt tattttaact tctggatttc
     2761 ttacatcaac attaatccta tctcctacct ttgataataa gtatccacct atatccatac
     2821 tcatttcttg agaagtcaac ccgaaagact tatctcctct tcttgactct accttaaaag
     2881 ttttatatcc agcctctatt ttttcttcta acatttttaa agctatttct tttaatgtat
     2941 cataatcttt tttagctctt actccagggc acacacccac tatcccaaat acttttctaa
     3001 cttcttcaat tacttcttca taatcatagt cttctaaatc aacatatatt cttccatact
     3061 ctttatatac attaaatctt ccaattggtt ttaacatatt tcttatattt cttataagcc
     3121 tattctcaaa tatatatcta ttctttcctt ttactccaat ttctccatac tttactatta
     3181 atatattata caccatttca cctcttaaac taatttatta tcactatcta tacagtattc
     3241 tttattttct tttcattatt gaccttaagt cacaaattga ttctttcaaa actttcacag
     3301 cttctaacat ttcttcttct gtattcatat cagataaact aaatcttatt gttccattta
     3361 tttcttcatt tgtaagacct atagcattta atacgtaact acctttcttt ttagaagaac
     3421 atgcagagcc agttgataca taaatacctt tttgctctaa gtaatgaagt aaaacttctc
     3481 cttttgtccc tctaaatgat acatttaata tatgacatac tccatcttct ggagaattaa
     3541 tttttatatc ttctatatta tcaattattt cattttttaa tatatttttt aagttattta
     3601 ctttcgaaat aacagcatct aaatctttgt ttagtattct aacagcttct ccaagaccat
     3661 atattccagg tacattttct gttcccgacc taattccaat ttcctgacca ccacctgtta
     3721 acataggctt aaggcgatta ttttctttta tatacataaa tccaattccc tttggaccat
     3781 gaaatttatg tgcacttaca ctcataaaat ctatattgta tttagatggt ctaaagttaa
     3841 tttttccata tgactgaata gcatcaacat ggaaataaat tttttctttt aaacccttca
     3901 aatactttcc aacttcacct attggctgta tagttcctat ttcattgtta acatgcatca
     3961 tgcttactaa gcatgtagtt gactttatcg cattttttaa atcctctata tttattttcc
     4021 catcttttcc gacctctaaa taagttactt caaaaccatc ttcttctaaa tcttttaatg
     4081 tatttaaaac agaaggatgt tctatgttag tagatattat gtgatttttt ctttttttat
     4141 ttaaactagt aatacctctt attatagtat tattacactc tgtaccacct gatgtaaaat
     4201 aaatctcctt ttctttcgct cctagagatt tagctatatc ttgtcttact tcttttatag
     4261 ctttttcaac ttctacacct tttctatgga cagaagaagg atttccatat tccgtattaa
     4321 gtgcataaac cattttatct ataacttctt gataaggttt tgtagttgcg ctattgtcta
     4381 agtatatttc caacttaatt acacctacct ttatttaaaa tcttcaactc taactattat
     4441 accactaatt atagtatttt ttttgttaat ctttattaaa aagacaatct taaattataa
     4501 aatattattt catgaaaagc tagttttttc actttattac aaatttttat agtaaaatat
     4561 aatatactta tactataccc tacaaaaata tattataaaa tacaatatat gataaaataa
     4621 tatattatat aaaataaata tattatatta tacatatact tatttttact ataaaagtta
     4681 aatttataag cttacttgtt ataaaaataa cttttataat ttatttcaaa aaaaatattg
     4741 atttttgtgt cgcattttgt tataataaat taaggttatc cccctggtaa ccctagttag
     4801 ttaatatatc tattcccaat accctttcat aaggatatat tttatattta ttaaaaatcg
     4861 gattagtaac ggaaattaat tcggtggaaa aaaagagctt accccgagct cttttttttc
     4921 tgcaaaaaat cgtaaaaata aagttaatat taatataact ttaattttaa aattaatatt
     4981 aaccatcact aaaaaaggat gtattgaatt tctaatatca ataacatcct ttttatataa
     5041 ttatagcact tttgctaaaa atgcttttgt tctttcatgt tttggatttc cgaaaacttc
     5101 ttctggagta ttatcttcaa gtatacttcc tccatctata aaaataactc tatctgcaac
     5161 ttccttagca aatcccatct catgagttac aattgccatt gtcatacctt ctttagcaag
     5221 ttcttttata acatccaaaa cttcgtttac catttctggg tcaagagcag atgttggctc
     5281 atcaaacaac atcatatctg gttccatagc aagtgctcta gctattgcta ttctttgctt
     5341 ttgtcctcca gataattgtg atggatatga atctttttta tctaaaagac caactctact
     5401 caacaaaatt tctgcttttt tttctgcttc ttccttagtc atctttttta atttaattgg
     5461 tgctaatgtt atattatcta aaatagtctt atgtgggaat aaattaaagt tctgaaacac
     5521 catacctata ttttgtctaa cctcatcaat attagttttt ttatcgacca aattttttcc
     5581 ttcaaatatt atttctccac ctgtaggtat ttctagtaaa ttcatacatc taagaagtgt
     5641 acttttacca gaaccacttg gacctactat aaccattatt tcgcctttag cgatttctaa
     5701 gtctatattt tttaatacat ttaaatctcc aaaagattta cttaaatttt ttattgtaat
     5761 catattatca ctttcctttt tactattcta tgttttccct aaatgctaaa catgctctca
     5821 tctagttaga cacagatagt ttcttttcta ataatccaac taatcttgat agagtaaatg
     5881 ttataacaaa gtaaattgct gctgctacta ttaatggctc taatgctcta aatgctgcat
     5941 tttttactat atcagcagta cgcattatct caacaactcc tattacagat attattgaag
     6001 attcttttac aagtgtaata aactcatttg ctaatgaagg taatatattt tttatagctt
     6061 gaggtattat tatatatctc atagttgacc aataatttaa tcctaatgat ctacttgctt
     6121 ccatttgtcc attatcaact gattgtatac ctgatctaag tatctctgcc acgtatgcac
     6181 cagagtttac tgataatgca aatgcactag ctataaattc acttggtata ccaaacaaca
     6241 tagggaatct tattcctaat tgaggtaaac caaaccatac tatatatatc tgtactaata
     6301 atggtgtccc tcttaaaacc tcaatataga ttgatgatat tgctcttaat actttgctct
     6361 ttgatatttt tgccatacag ataattatac ctattataaa tccaaagcat aatgccacaa
     6421 gagaaattga tactgtcaca cctgttcctt ccaaaaaaga agtcccaaat ctacttaaaa
     6481 aactaaaatc taaactcata atttcatctc ccacataact ttcttactaa tttgaagcag
     6541 cttcagtaga tgcttcattt aaaaattctt ctatcttctt gccatcttgt aattctttta
     6601 tctttttgtt taataattct ataaaatctt tgttattgtc agattttttg attgctgctg
     6661 ccataccttc tgttacatct ttcccaactt cagtatttgc catttttatt ccatcatatt
     6721 ttttaacatt tattaaagaa actgcttcat tagtaacaac tgcatctatg tttccatttt
     6781 ttaattctgt aattaaatca ggtattgctt tcaaagattt tgtagttgtc atttttaaag
     6841 tgttaactac atattcttct tggatacttc ctgcttgaac tccaactcta agtttcttta
     6901 aatcatcttc agtttttact ttgtctatat ctgcatcttt aactataact gcatttctac
     6961 ttaaataata taattctgaa aaatcaacac tcttttctct ctctggtgtt ggagacatac
     7021 ctgcaagaac aatatcaact tggtctgcat ttaaagcacc tattaatcca tcaaatgaca
     7081 tatccttaat ttcaaccttt acaccaagct cttttgcaaa ttcttcagcc aacattatat
     7141 cgaatccttt tattgaatct tttccatcta ctaccttatg aaattcaaat ggaggaaatt
     7201 ctgcactagt accaacaact aattttccat tgtcttttat ttgttctaac tttgtttttt
     7261 ctcctccacc actacatcct actagcgata atgtcaatcc taatactaat cctaaactta
     7321 ataatttttt aaatactttc attttaacta cccctctctt ttaattataa tttccctcag
     7381 attttaatac ttaaatgttt cgagttacaa aaaaagtctc tcagtcttta atgactaaga
     7441 gacgacttta ccgcgttacc actcttattg ataaatactt atattacttt caaaatttgt
     7501 gactgttagc atttaagctg tatggatatt tttattttta aatcaaaaaa gtcccttagc
     7561 tttacagcta agagacgaat tatatccgcg ttaccactct ttttgataag taagtactta
     7621 tcctctcaat cccttaaggc gggtaaacca attatcatac taaagtttct gataatctcc
     7681 tccagagctc tcttcacata gacttcattg ctaggcttac accaacccct aactcgctgt
     7741 aaatccatat atgctacttt ctctatcatt ggatttgttt ccttaaattg ttaatacaat
     7801 tgtacttcct tataatattt ttgtcaataa gttttttatt tttttaataa tttttacttg
     7861 taaattatct aaatactact gtttatattt attgatatta gttttgtaat tataaatagt
     7921 atatttcata tttttagcta tttcttaaat aatactaaat aatatctttc acattgttgc
     7981 tttttcttaa attacactaa ataatatctt tcacattgtt gctttttctt aaacaatact
     8041 aaataatatc tttcacattg ttgcttttct taaattacac taaataatat ctttcacact
     8101 gttgcttttt ctttaataat actaaatagt atctttcaca ctgttgccct ttctttaata
     8161 atactaaata atatctttca cattgttgct ttttcttaaa ttacactaaa taatatcttt
     8221 cacactgttg ctttttcttt aataatacta aatagtatct ttcacactgt ttctctttct
     8281 ttaataatac taaataatat tttccacatt gttgcttttt ctaaacaact ataaataata
     8341 tattaaaccc ttatttcttt ttctaataag ttaaataata cattttacac atatctattt
     8401 gtcttgcaca taataaataa tacattatat ttctatctaa atttagattc taaattaaat
     8461 agtataaatt atattcttca ataaacaagt tttcaaatcc aagattcgct ctctatcttt
     8521 aaactttatt gtaaacataa ttccatcttt tttaattatt tctccttcat gaaatttttt
     8581 atggtatacc ttatccccta caccaatact gtcaatttct tttttcgttg gagattttat
     8641 atcattaata aataatgatt tatcaacttt cttaccatac ttatttaaag tagagcttat
     8701 acataacttt tcttcagctc ttgtaatagc aacatacata agtcttcttt cttcttctaa
     8761 ttgttcttct tttttctctt ccttacaaat atcatatgat ttttcatgag gtattgttcc
     8821 ttcatttaca cctataatat aaacatttct aaattctaat ccttttgcac tatgcatagt
     8881 tgtaaatatg acaccctcag tctgcttatt cttgttattt tccgttaact cagtctttac
     8941 tctatcaata tgctctaaaa actctgtaac agtattaaaa ttagttgctg aactctcaag
     9001 ttcatttaat atctctacaa gtccattagt ctttatcttt ctgttgctac aataatctaa
     9061 aacatatcta tcataatcaa gactagttct aatataagat attgcatttt taggatttaa
     9121 agtatttaaa taacttaaat ctatttccaa atcatttatt gttttaactt gtttaggatg
     9181 aagattacac ttatttatta atgctgttat aaagtctttt tcatccttaa ccatatttat
     9241 actgtctttg gatatatatc taaaaggctt gtttataatt ctcaaccaat ctttatttga
     9301 ttttggatta atacctattc ttaaataggc tagtatgtct tgacttgccc aatggtcata
     9361 tattgtaatt actgagtctt ttactacaaa tggaatcctc atatccataa atacatctac
     9421 taatgctctt gactgtatat tagttctata aattactgca aaatctgaat actctacata
     9481 atctttttgt atttcgtcaa ttatttccct ggcaatatat aaggcttctt cctctgaatc
     9541 atgcggagaa atataggtaa cacttcctcc atccccctga ctacacttaa tgattttttc
     9601 atatctattt ttatttttgt cgataagtct atttgcagta tgcactatct cacttttaga
     9661 cctgtaattt atatctaata ttattttttt agtattatta aagtattgtt caaattcaag
     9721 caaaaaatct ggtctagcac ctctaaatcc atatatactt tggtcttcat ctccaactgc
     9781 aaatatattg tttaatgggc tacaaattaa ctttaaaact tcaaactgta ctttatttat
     9841 atcttgaaat tcatctatca atatatattt atacacattt ctaaccattt ctagtgcaga
     9901 cttattgttc aaaagtaaat aataagttct tataagcata tcatcaaaat ctattttatt
     9961 aactttagat ttttgttctt cgtataagtt gtatactttt aaaaactcat cttttgttaa
    10021 tacttctgaa ttaaattcat ttttatccat aagttcattt tttacatagg aaatttcatt
    10081 tataacttgt cctatattct catcatcatc agcattctct atatttaaac tttttaggat
    10141 tgccttgatt gtcattcgct ttgacttttc atcaaatata ctgtctaagt tgtatctttc
    10201 aaagtatctt agaattttaa aaaatacaga gtgaaaagta ccataggtta ccttattaag
    10261 tcttatgtca tcacttaaat tcaaagctct atttttcatt tctattgaag atgccttggt
    10321 aaaacttatt gccaagattc ttgttggtgc tatgtttttg tttacaacca tattggctat
    10381 tctatatgta attactctgg tcttacctga accaggccct gcaagtatca tgcaaggccc
    10441 atctatatgc tcaactgctt ctaattgatt ttcatttaac tttcttatat ctatatttgt
    10501 tgtgattatt agctccacca ccttgattta taattatatt attttctttt taattataaa
    10561 ctaaaagttc ataaaaataa atttaatttt tcctctatta aaccacatca ttcctatttt
    10621 catgacatat cttgagttct tttttaattt tgtcgcttat atcttcaggt ataaatgatg
    10681 gtttactaat atagtagcct tgcatataat ctactccaag attaattagt gtttccattt
    10741 caccttttgt ttctacacct tctgctatca ctttaatatt tctttcgtta gaatatgaga
    10801 taagattttt taatattttc tgtctgtttt catctttatc aatacctctt acaatagaca
    10861 tatcaatctt tacatagtct ggcattatat ataatagtac agcatctccg ttgtaacctg
    10921 tcccaaaatc atctaaagca atttctatat tccaatggtc tatatatgct ttctttttct
    10981 caataaaatc ttcattaatc ttatcattct ctgttatttc tagtactact ctatttaaat
    11041 attcaccata taaatcttca atttcctcaa aatctacttt tgacaaatta ttattaggaa
    11101 tagaatttaa gaacagtttg caatcgccaa atttttcttt gtatttttta aatgatttaa
    11161 gtgctttaaa ccatgtaata cgctcaattt gatataattt agactgtgaa cgagctaatc
    11221 taatgacatc tattggcgaa ctaaaagttt ttattttagg tctcataagt gcttcatatg
    11281 caaatacttt acctttacaa tctacaatag gctgaaatgc atactcaact aattgaccat
    11341 caattaactt gtttaattct tctctattac tcaataagaa tgaatcctta ttatatatat
    11401 taatatcaaa ttcactaact tcacccttga ttgtattttt aatttgatac atagcaaaat
    11461 ctgcgtatct cattaattca tcatagttgg ttgaatcatc agggtaccag gaaattcctg
    11521 ctgatgccct tattttaaat tctgttccat catgaagttt taataaggta ttattcatct
    11581 gtttcttaac agaattaata atatctctta tactatcttt actttcatat ccatatataa
    11641 atacataaaa ctcatcacct gaaagtctag atactatacc attccatgat ataaacttct
    11701 taagtatgtc tgcagcacac ctaatatatt catcaccagc atcatgtcca tatgtatcat
    11761 taatatattt tagattatct aaatcccaca taataaatgc tgctatctta agttcatctt
    11821 tattcttaaa tttttcttgt atctgtgaat gaaaagctct cctatttaaa agattcgtta
    11881 aaatatcaaa atctctttca tattctatct tccttttctc aataaattct tttgttacat
    11941 caacaacaac accaagaacc tttagctcat cttcaaagat attcattctt atccacttaa
    12001 gttttttatc tttagattca aacttatata tatatgtatt ttctgtttca taactttctt
    12061 gcaaatattt atctagtccc cttaattttt tcaggaactt atttttttcc atatatccat
    12121 attcttcaaa tttgatttca gtagtaaaca cgctaaaaaa ttcttttgta caaaaaacat
    12181 tctctgcgtt tttgtataac tcaaaagctc caattggcat atctaccatt tcaataatct
    12241 gtgataattt tgacgaagaa tctgcaacat ttgcactgag caactctata gctgaagata
    12301 attcatctat ttcagatata tttatcttat caagatttac tggttttgat ggattactgt
    12361 ttctaacttt gttggctaaa agaacaattg gttttgtaaa taatcttgat gcaatgtaaa
    12421 tacctattag acctattaat aaagaaattg ctatagatat tataactaat ttctctaccc
    12481 tttttgaaaa tgacaaaaga cttttttctt caataatacc caaaagagcc caacgttcat
    12541 tttcaaatgg agtattggag ttatataaat ctaagtattg tacactagca taaatgttgc
    12601 cattaccaca ttcattatct ttatttttaa tcttatatat atttttatac gattccttct
    12661 gctctaaatt agaatgtaaa ccatcccaaa atttttcttt taatgagtat ccatttgaaa
    12721 ttacattttc aaaggccata tcattatttc tatctacacc taaaatataa ttaccctttt
    12781 tattcccatt tatctctgag tatggaagta attctcttaa gtaatttaca cttaaatcta
    12841 ttccaagtac tccatataca gttccatctt cataaataag gggaactgta tatgtcataa
    12901 gcctatcacc tgcatttgtc aataaaaact tacttcccca gtatcctaaa ttagaacttg
    12961 atacatttgg attatcaatt gcagctctaa atggtttata aaaaaaatca tctccatctt
    13021 gctcattttc acttttaaat gaaaacttag gttcccaata actgtccata gatattccaa
    13081 aacttcttgc tatatctgat gaacctctct caattaacaa atcagaatta tcatttggat
    13141 taaacttagg gtctaaatcc cttatataca atcctgtcct acttatttca cctttactat
    13201 ctattgactt atcgttaata tttttgtcac ttaggattat aaaagctcct gtaacagaat
    13261 tttttctcaa aagatatatt aaatcatttg atatgctttt tataatatct ttgttaattt
    13321 cttcattgtt gcctacatcc tcaattaagg catttttttc atttaatact ttttttacag
    13381 aggaattaat tgtagcttcc gtttcacttg tatttgacca tctttgtaac atttcatttt
    13441 gtatataatt tttacgatta attactcttt catttaagat gtcaaacgaa ttatcattta
    13501 atttcttaat agttcctccc cataaaattg tggcacaaaa taaacttgtt tgaattagca
    13561 taacaataat taggggaaat atcatctttt tcattattga attgtttttt cttagattgt
    13621 tttccatacc cattccacct atacctttgt gctcgtcaac acactaactg ccaattcatc
    13681 tattttttta tagcattttc aagctcagcc ttaaaacttt caaaccaagt tttaaaattt
    13741 ttatcattat taaattgtgc tactgcatcc ttcttagaag ttccactttt tactaattca
    13801 tttattttat ttgaatcttc tttagcttta tcaagcattg aatattcaag tatttgtcta
    13861 gctttagttc caccatcaaa tgctttatta gtatataaat tacaactgtt aatctgttca
    13921 atagatgagg ctattgaacc ttcaacttta tcagataaac tcaaattatc tttcttcata
    13981 acttctttta taacatccaa atcattcgtt tttttcttta ctggtaagta tccagaagca
    14041 atagagaatt ttatattagg ttctttatct gtaaaccatt ttaaaaattc tactgatgca
    14101 tattcttctt ctttagtaga ttttgttaca gccattcctg caccttgttg tacagcatat
    14161 tttccttctc ctttaaaacg tggtgcttga aaaacagatg tttcaatatt ataactctca
    14221 ttatcattta gcattactct atctggaaag tatgctgctc ctgaactaga acctacaaga
    14281 gctaatatat cacctgtttt tgcatcatca gaacgaaact taccataaga agcaaaatat
    14341 ccatttatat atggtacata gaaattatcc catagttttt tcataacttt ttcatcaata
    14401 tttagtgtaa ctttaccatc tttaacagaa aaaatttcta ctcctagttg tttacttcct
    14461 ataataatat aatttgctaa agcatctctt ccaaaaaatg ctttaccatc attaggctct
    14521 ttcgttaaac tatctgtcca ttcataatat ttttttgatg tctctactaa tccttcaatt
    14581 gtttttatat catttatatt tgaatttgta gcttttgcaa atttatccca atcagtttta
    14641 tttaacatca aaatttcagt tgattttgca attgggaaaa gtttaaactc atccttagaa
    14701 tttattctgc cttcttctac atattcgtct atgtattcat ttaattcttc tttacttatg
    14761 tacttatcca tatctactag taaccctaat ttatccattt catatgcagt atctgagtat
    14821 gctgcaaata tatttggtac atcttcagaa ccaacttttt tatttgcaga atctaaaact
    14881 ttctctgata aatcaactac attaccttga ctgaatgctt caacaataat tcctttttca
    14941 gcacctacag tgtcattaaa ttccttaaca agcttatcaa aagccatttt ctgctgtcca
    15001 ttataataat gccaaatttg tattgatgtc ggattttctg ggtctaactt ataattatct
    15061 tccttcttta aactacatcc tgtaagtaat aaaacaatca ttgatgtgat aattaatttt
    15121 gcaaacttac acctattcat aaaatctact ccttcaaatt ataaaattta taatagcatt
    15181 aaaactgtaa cttcactatt tatattaatt atagaacgtt tattgatata tttaaacagg
    15241 atttacaaca tatttaaaag caattaagaa acatactatt acaaatatag aaaataaagc
    15301 ttttacataa tttttatttc taaatgcgcc aaatgacacc aatccttgtt gaatagctac
    15361 tgtaattact aaaagtatca ttattatatt aagttgaacc agtttaaata agtaaagcaa
    15421 tgaaaacact actagaagta cacataatac aatactagat ttattaataa tatcatcaat
    15481 cttatctacc ttcttagatt gcttattaac tttatttaat ttttctttat ttaaattctc
    15541 aactttttta ttataccttt ctcttgtttt atctttatat ttagtatttt tatttttatt
    15601 tcccatttat tttccatcct ctataaattt tttaattgag cttatacatt atttaaatat
    15661 tatatattta agataccata tattcctaca aaaaggtaac ttactatgta tctatttttg
    15721 attttcgcat aaaaaagcca tctcctttag aattaattct gtattgagac agctaattta
    15781 actatttatc ctttgaaaca ttttctacat tatcatattt actatcaact aaaactatat
    15841 caactcttct attttttgac ctaccttctt cagatgaatt ttctgctatt ggtctaaact
    15901 caccatatcc tacagcagat aatttatctg gtgctatacc tgcattgtca ataagaagtt
    15961 gaacaacatt ggttgctctc ataactgata aatcccaatt tgacttaaac tctgaatttt
    16021 ttatagacat attgtctgta tggccttcta ctctcatgta actattcatt tcatttaaca
    16081 tcttacctat ttgaataatc ttatctctag aattatcctt cacaatcgct ttccctgtat
    16141 caaatagtat tgtatcttta aggcttatta ctaatcctct atcttgaact cttaatgata
    16201 ctgaattagc cataccattt tcatttaaat acttctggat ttcttcacca acttttttaa
    16261 acttagcatt ttgtaaagta tctaaatcat tacttcctgg ttcaactact ggttctatct
    16321 tgctattacc accttcaata actccagaag aaccaccaaa agcagaattt agagattgag
    16381 ataattgttt atatttctcc gcatcaacat tactcataga atacattatt acaaagaaaa
    16441 tcataagtaa tgttatgagg tctgaataag ttaaaagcca tcgctcattg ttttcttctt
    16501 tttcatcttc atcatacaac ataaacattc catcctttta tattattcag ctctaccacc
    16561 aatttcttca agttcaatta gttcttttga atttaaaaat atttttaatt tttctgtcat
    16621 taaattagaa ttatcacctt caagtatgga taatattgct tctatcacca aaagtctttc
    16681 acttatttcc tgttgattta tagccttaag ttttgatgct attggtaacc atattaaatt
    16741 ggcagaacca acaccataca aagtagccaa gaatgcaaca gaaatttgag gtcctaatgc
    16801 atctggatta cttgaaagat tacctaaaat atgtactaac cccataactg tacctattat
    16861 ccccagtgta ggtgcatatc cccctgctga atcaaaaatt gctattcctt ctttatgtcg
    16921 ttttgatgtt atctgtatct gtgattccaa tatatttctt atagtaactg catccactcc
    16981 atctaccata agttcaagcc cttttctaat aaaagggtct aattcatcat tctcacttat
    17041 ttctttgtca atactaagaa gcccctcttt cctagccttt gttgcaattt ctttgaaata
    17101 gattatattt tctatgttat taggcttttt atttttaaaa gctaataaca ttatctttat
    17161 agacttttta aacacaggga atggagtcga agctccaaca gctcctatag ttccaccaaa
    17221 cacaacaagt gctgatgaaa atacaaataa tgctccaaca tgtcccccat caataacaaa
    17281 agcaactacc aaagatgcta tccccactat gaaaaaaatt aaagttgtca tctattccct
    17341 cctttcttta gttatacata tctaatcatt tatgacatta taaaaagact atctttaata
    17401 aaaatagcct ttaataattg gcggagaagg agggattcga accctcgcac cggttaccca
    17461 gcctaatccc ttagcagggg atcctcttga gccacttgag tacttctcca tatctctata
    17521 ctagtaaatt atataataaa atcatatttt agtcaattgt tttataaaat ttgagttatt
    17581 ttgaattatt aaacatctat ttttactttt ttgcttaaat gtaagtttta aatatggtaa
    17641 aattatcttg cataacactt ataatattat ttgataaata tacatacatt aacaaatata
    17701 cctaaactct gacttaattg taagttatgc caaaactaaa ataatactag tccaaggagg
    17761 atattttatg ttaccacagg aaagatatga gaagatttta agtattctca aatcagataa
    17821 tataattaaa ataaatgaca tagttcaaat gtttgatata tcaatagaaa ctgcacgaag
    17881 agatttagcc catatggaga gtatcggtct tttaaaaaga gtttatggtg gtgctatacc
    17941 agttatagca agtagcgtag aacttaacta tgttaaaaga aaaaaaataa actcaaacga
    18001 taaaaaacag attgctatta aatgtagtga gtttataaat acaaatgata ctatttttat
    18061 ggatactgga actactgtac ttgaagtttc aaaaaatttg aaagataaaa agaatctaac
    18121 tgtaataacc aactcaattt tagttgcaaa cgaacttatg aatactaata taaaagtata
    18181 tgtgcttggg ggctatctta gagaaactga aggttctttt tctggtccgc ttacactgtc
    18241 agctattgaa caatttaatg ttgataaagc tattttaggc gctggaggta tcactcttga
    18301 aaatggaatc tctgactatc acttagaaga agcacttgtt agaaaaaaaa tgattgaaag
    18361 gtctagaaaa tctatagtag ttgtcgaacc tcctaagttt ggtataaatt catttgcatc
    18421 agtagctcct atagaacata tagatactat tattacctct gctagtatag accataatat
    18481 aataatagag ttttctcaaa aaggaatacc tttagtcata gctgataatt agcttatatg
    18541 attgattttt agttccttga tgtaataaat taaaattaag gagccaaata attatttatt
    18601 tagctccatt tatgtctaaa ctatacttta tattttaatt atgtaactac tttcagttag
    18661 cttatcattt ttagttattt gattgccatt ttatttaatt tataagtttt agttattcga
    18721 ttatttttta attgtctgtc tttttaattt atttttagtt atttaaactc ctactttaaa
    18781 ataatttaaa tttgacttaa gtattgttta ttctacactg catctttctt cattttaata
    18841 ctttgttctt cttccaaggt taaatctttt gtcataagta tagcaagtgc aattgccaat
    18901 actcctccaa ccaattttcc aattacaaca ggtgtaatca tctctggaac tactcctgct
    18961 gtaaatccta aatggtcccc caaagctgct gtagcacaaa ccagccaagc agtatttact
    19021 actttcccct ttggactcat atctttcatc attttatata ctggaatact attggctaaa
    19081 gtaaaaacta atcctgctgc actagtggca tccataccta attttccacc cactattgta
    19141 agtggtttat taagaacttt aactaataaa tgtaatattg gaaaagtacc taataacacg
    19201 attgcaatac ttccaattac ttgaatacct tctcttatag gtgtcatgcc ttttataagt
    19261 actatacctg ttatttcttg aaatgctgct gctcccaaac ctatcgttat caatatagat
    19321 ataaattttc caaaaattaa tgacccattt atcataagcg tagaattaaa ttttaaacct
    19381 actgccaata tcactgccaa tattatcact ggtatcatat ttataattac cattgataag
    19441 ttaaatcctg ctattaatcc tcctataata cctccaaaag gtattgttat caagcctatc
    19501 agtaaccctt ttgcaaagaa tggtctgtct tcatattcta taagacctag tcctactgga
    19561 attgaaaata ctaatgtaca tccaagcatg gatgacacaa ccaatcctgc aagcattcct
    19621 gcctctttat tgtgtgcaag ttccatagcc aatggatacc ctcccatatc attcgctaaa
    19681 atagttgcaa acatagatgg gtcagctcca cagagattga agattggtat tattatagga
    19741 cctagtacat ctgaaattac tggagctaaa caaactatac caaccattcc taaagccaat
    19801 ggacccattg aattaaaccc ttcttcaaac tgctctccaa gtccaaattt atttccaatt
    19861 attctatcta acgctcctaa aagcactccc acagccatta tgtataatat aattttatca
    19921 aagggcattt ttaattcctc ctatacatgt aaaatactct attgtaactt aattagcaca
    19981 cacctttttg gtatcgtttt cccaattcat agctcttttt actgcttgat tccatccaag
    20041 taatatttct tctctttttt gattagtcat atttggaata tattttttgc ttattgtaaa
    20101 ttcacttcta atttcatcaa tactgttcca gacaccacaa gaaagacctg ccatatatcc
    20161 tactccaagt actgtcgtct caggattttc tgcaatttca attggtatac ctaatatgtc
    20221 ggattgaaat tgcattgtaa agttattttt agtaattcca ccatctactc tcaacatttt
    20281 tatttttttc cctgaggcta agttcataga atcaacaaca tctttgactt gaagtgcaac
    20341 agactcaaga gtcgctctta ctatatgctc ttttttagta cttcctgtta tcccaaccat
    20401 tgtacctcta gcatattggt cccaataagg tgctgctata cctgcaaatg ctggtacaaa
    20461 atatacacct ccagtacttg atatggaatt tgccatgtca tcagtttctg aataattttt
    20521 tataactcca atgccatctc taagccattg tattgcagca cctgctatat acactccacc
    20581 atcataagca tatgtaactt tatctcctat tttccaagca acagttgtca aaataccatt
    20641 acctgctata tttagttcct cacctatatt catatatagg aaacatcctg taccataagt
    20701 atttttaacc attcctggtt caaaacatcc attgccaaat aaagctgctg gttggtctac
    20761 cattgaagca gtaattttta tagaaacccc acaaaatgcc tccttatctg tagttccaaa
    20821 atcaccactc gtaggcatta tatctgctaa aatacttcta ggtattccta taatgtcaag
    20881 caactctcta tcccaatcca tatctcttat attaaaaagc attgttcttg ctgctgtaga
    20941 tacatcagtt aaaaaagatt ttcctcctgt cattttccat attaaccaac tatctaatgt
    21001 tcctgcaagt acttctccat ttttaatttt ttcttttaca ccttttacat tatctattac
    21061 ccatttaatt tttgtacctg agaaatatgg gtctataatt aacccagtct tatctattat
    21121 cttattttct aaacctggta tattctttaa attttccaca taatcataag ttctcctgca
    21181 ctgccacaca atagcattgt aaagtggttt acctgtattt ttatcccata acattactgt
    21241 ttcaccttga ttatctatac caattccttt tatatcactt tcattgattc cattttcaaa
    21301 agcctgttgt aatacattat tactcacagt ttttgtatta ttccaaattt ctatagggtc
    21361 atgttcaaca tatccagatt gtggatatat ttgagtatgt tccatataat cgctaaaaac
    21421 aaactctgat ttttcgttta tcaatactgc tcttgttcct gttgttcctt ggtcaattgc
    21481 taatacatat ttcactttaa atgtccccct ttattttcag aattaacaaa ttctttttta
    21541 ttttttgaat atataatcta ttcaagttat ttatatttta tatcatcatg tggatttatg
    21601 caatattcat tacaatatt
//
LOCUS       DAGSRM010000066        20419 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.066, whole genome shotgun sequence.
ACCESSION   DAGSRM010000066 DAGSRM010000000
VERSION     DAGSRM010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20419
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.066"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            41..595
                     /gene="tcdR"
                     /locus_tag="MJE33_002562"
     CDS             41..595
                     /gene="tcdR"
                     /locus_tag="MJE33_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin sigma factor TcdR"
                     /protein_id="HBY9648492.1"
                     /translation="MQKSFYELIVLARNNSVDDLQEILFMFKPLVKKLSRVLHYEEGE
                     TDLIIFFIELIKNIKLSSFSEKSDAIIVKYIHKSLLNKTFELSRRYSKMKFNFVEFDE
                     NILNMKNNYQSKSVFEEDICFFEYILKELSGIQRKVIFYKYLKGYSDREISVKLKISR
                     QAVNKAKNRAFKKIKKDYENYFNL"
     gene            913..8013
                     /gene="tcdB"
                     /locus_tag="MJE33_002563"
                     /gene_synonym="toxB"
     CDS             913..8013
                     /gene="tcdB"
                     /locus_tag="MJE33_002563"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_074049122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HBY9648493.1"
                     /translation="MSLVNRKQLEKMANVRFRTQEDEYVAILDALEEYHNMSENTVVE
                     KYLKLKDINSLTDIYIDTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVW
                     IGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTVVESAINDTLESFREN
                     LNDPRFDYNKFFRKRMEIIYDKQKNFINYYKAQREENPELIIDDIVKTYLSNEYSKEI
                     DELNTYIEESLNKITQNSGNDVRNFEEFKNGESFNLYEQELVERWNLAAASDILRISA
                     LKEIGGMYLDVDMLPGIQPDLFESIEKPSSVTVDFWEMTKLEAIMKYKEYIPEYTSEH
                     FDMLDEEVQSSFESVLASKSDKSEIFSSLGDMEASPLEVKIAFNSKGIINQGLISVKD
                     SYCSNLIVKQIENRYKILNNSLNPAISEDNDFNTTTNTFIDSIMAEANADNGRFMMEL
                     GKYLRVGFFPDVKTTINLSGPEAYAAAYQDLLMFKEGSMNIHLIEADLRNFEISKTNI
                     SQSTEQEMASLWSFDDARAKAQFEEYKRNYFEGSLGEDDNLDFSQNIVVDKEYLLEKI
                     SSLARSSERGYIHYIVQLQGDKISYEAACNLFAKTPYDSVLFQKNIEDSEIAYYYNPG
                     DGEIQEIDKYKIPSIISDRPKIKLTFIGHGKDEFNTDIFAGFDVDSLSTEIEAAIDLA
                     KEDISPKSIEINLLGCNMFSYSINVEETYPGKLLLKVKDKISELMPSISQDSIIVSAN
                     QYEVRINSEGRRELLDHSGEWINKEESIIKDISSKEYISFNPKENKITVKSKNLPELS
                     TLLQEIRNNSNSSDIELEEKVMLTECEINVISNIDTQIVEERIEEAKNLTSDSINYIK
                     DEFKLIESISDALCDLKQQNELEDSHFISFEDISETDEGFSIRFINKETGESIFVETE
                     KTIFSEYANHITEEISKIKGTIFDTVNGKLVKKVNLDTTHEVNTLNAAFFIQSLIEYN
                     SSKESLSNLSVAMKVQVYAQLFSTGLNTITDAAKVVELVSTALDETIDLLPTLSEGLP
                     IIATIIDGVSLGAAIKELSETSDPLLRQEIEAKIGIMAVNLTTATTAIITSSLGIASG
                     FSILLVPLAGISAGIPSLVNNELVLRDKATKVVDYFKHVSLVETEGVFTLLDDKIMMP
                     QDDLVISEIDFNNNSIVLGKCEIWRMEGGSGHTVTDDIDHFFSAPSITYREPHLSIYD
                     VLEVQKEELDLSKDLMVLPNAPNRVFAWETGWTPGLRSLENDGTKLLDRIRDNYEGEF
                     YWRYFAFIADALITTLKPRYEDTNIRINLDSNTRSFIVPIITTEYIREKLSYSFYGSG
                     GTYALSLSQYNMGINIELSESDVWIIDVDNVVRDVTIESDKIKKGDLIEGILSTLSIE
                     ENKIILNSHEINFSGEVNGSNGFVSLTFSILEGINAIIEVDLLSKSYKLLISGELKIL
                     MLNSNHIQQKIDYIGFNSELQKNIPYSFVDSEGKENGFINGSTKEGLFVSELPDVVLI
                     SKVYMDDSKPSFGYYSNNLKDVKVITKDNVNILTGYYLKDDIKISLSLTLQDEKTIKL
                     NSVHLDESGVAEILKFMNRKGNTNTSDSLMSFLESMNIKSIFVNFLQSNIKFILDANF
                     IISGTTSIGQFEFICDENDNIQPYFIKFNTLETNYTLYVGNRQNMIVEPNYDLDDSGD
                     ISSTVINFSQKYLYGIDSCVNKVVISPNIYTDEINITPVYETNNTYPEVIVLDANYIN
                     EKINVNINDLSIRYVWSNDGNDFILMSTSEENKVSQVKIRFVNVFKDKTLANKLSFNF
                     SDKQDVPVSEIILSFTPSYYEDGLIGYDLGLVSLYNEKFYINNFGMMLSGLIYINDSL
                     YYFKPPVNNLITGFVTVGDDKYYFNPINGGAASIGETIIDDKNYYFNQSGVLQTGVFS
                     TEDGFKYFAPANTLDENLEGEAIDFTGKLIIDENIYYFDDNYRGAVEWKELDGEMHYF
                     SPETGKAFKGLNQIGDDKYYFNSDGVMQKGFVSINDNKHYFDDSGVMKVGYTEIDGKH
                     FYFAENGEMQIGVFNTEDGFKYFAHHNEDLGNEEGEEISYSGILNFNNKIYYFDDSFT
                     AVVGWKDLEDGSKYYFDEDTAEAYIGLSLINDGQYYFNDDGIMQVGFVTINDKVFYFS
                     DSGIIESGVQNIDDNYFYIDDNGIVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSG
                     LVRVGEDVYYFGETYTIETGWIYDMENESDKYYFNPETKKACKGINLIDDIKYYFDEK
                     GIMRTGLISFENNNYYFNENGEMQFGYINIEDKMFYFGEDGVMQIGVFNTPDGFKYFA
                     HQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVI
                     SE"
     gene            8135..8635
                     /gene="tcdE"
                     /locus_tag="MJE33_002564"
     CDS             8135..8635
                     /gene="tcdE"
                     /locus_tag="MJE33_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like glycosylating toxin export protein
                     TcdE"
                     /protein_id="HBY9648494.1"
                     /translation="MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILT
                     ISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQD
                     CINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAILLDAMTDEM
                     NAKDEK"
     gene            8964..9146
                     /locus_tag="MJE33_002565"
     CDS             8964..9146
                     /locus_tag="MJE33_002565"
                     /inference="COORDINATES: protein motif:HMM:NF022413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648495.1"
                     /translation="MRDEGQNDIENKYQTLINDLIDSLKVIGKIYKEIKTLKKYKICL
                     QITIRQSPYLIEESIN"
     gene            9363..17495
                     /gene="tcdA"
                     /locus_tag="MJE33_002566"
                     /gene_synonym="toxA"
     CDS             9363..17495
                     /gene="tcdA"
                     /locus_tag="MJE33_002566"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105161403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HBY9648496.1"
                     /translation="MSLISKEELIKLAYSIRPRENEYKTILTNLDEYNKLTTNNNENK
                     YLQLKKLNESIDVFMNKYKTSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWI
                     GGEVSDIALEYIKQWADINAEYNIKLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEI
                     QNPQFDNMKFYKKRMEFIYDRQKRFINYYKSQINKPTVPTIDDIIKSHLVSEYNRDET
                     VLESYRTNSLRKINSNHGIDIRANSLFTEQELLNIYSQELLNRGNLAAASDIVRLLAL
                     KNFGGVYLDVDMLPGIHSDLFKTISRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENF
                     DKLDQQLKDNFKLIIESKSEKSEIFSKLENLNVSDLEIKIAFALGSVINQALISKQGS
                     YLTNLVIEQVKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIA
                     PYLQVGFMPEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLS
                     QLTEQEINSLWSFDQASAKYQFEKYVRDYTGGSLSEDNGVDFNKNTALDKNYLLNNKI
                     PSNNVEEAGSKNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSYFLS
                     DDGESILELNKYRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSLSNEISSFLD
                     TIKLDISPKNVEVNLLGCNMFSYDFNVEETYPGKLLLSIMDKITSTLPDVNKNSITIG
                     ANQYEVRINSEGRKELLAHSGKWINKEEAIMSDLSSKEYIFFDSIDNKLKAKSKNIPG
                     LASISEDIKTLLLDASVSPDTKFILNNLKLNIESSIGDYIYYEKLEPVKNIIHNSIDD
                     LIDEFNLLENVSDELYELKKLNNLDEKYLISFEDISKNNSTYSVRFINKSNGESVYVE
                     TEKEIFSKYSEHITKEISTIKNSIITDVNGNLLDNIQLDHTSQVNTLNAAFFIQSLID
                     YSSNKDVLNDLSTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDTINVLPTITEG
                     IPIVSTILDGINLGAAIKELLDEHDPLLKKELEAKVGVLAINMSLSIAATVASIVGIG
                     AEVTIFLLPIAGISAGIPSLVNNELILHDKATSVVNYFNHLSESKKYGPLKTEDDKIL
                     VPIDDLVISEIDFNNNSIKLGTCNILAMEGGSGHTVTGNIDHFFSSPSISSHIPSLSI
                     YSAIGIETENLDFSKKIMMLPNAPSRVFWWETGAVPGLRSLENDGTRLLDSIRDLYPG
                     KFYWRFYAFFDYAITTLKPVYEDTNIKIKLDKDTRNFIMPTITTNEIRNKLSYSFDGA
                     GGTYSLLLSSYPISTNINLSKDDLWIFNIDNEVREISIENGTIKKGKLIKDVLSKIDI
                     NKNKLIIGNQTIDFSGDIDNKDRYIFLTCELDDKISLIIEINLVAKSYSLLLSGDKNY
                     LISNLSNTIEKINTLGLDSKNIAYNYTDESNNKYFGAISKTSQKSIIHYKKDSKNILE
                     FYNDSTLEFNSKDFIAEDINVFMKDDINTITGKYYVDNNTDKSIDFSISLVSKNQVKV
                     NGLYLNESVYSSYLDFVKNSDGHHNTSNFMNLFLDNISFWKLFGFENINFVIDKYFTL
                     VGKTNLGYVEFICDNNKNIDIYFGEWKTSSSKSTIFSGNGRNVVVEPIYNPDTGEDIS
                     TSLDFSYEPLYGIDRYINKVLIAPDLYTSLININTNYYSNEYYPEIIVLNPNTFHKKV
                     NINLDSSSFEYKWSTEGSDFILVRYLEESNKKILQKIRIKGILSNTQSFNKMSIDFKD
                     IKKLSLGYIMSNFKSFNSENELDRDHLGFKIIDNKTYYYDEDSKLVKGLININNSLFY
                     FDPIEFNLVTGWQTINGKKYYFDINTGAALTSYKIINGKHFYFNNDGVMQLGVFKGPD
                     GFEYFAPANTQNNNIEGQAIVYQSKFLTLNGKKYYFDNDSKAVTGWRIINNEKYYFNP
                     NNAIAAVGLQVIDNNKYYFNPDTAIISKGWQTVNGSRYYFDTDTAIAFNGYKTIDGKH
                     FYFDSDCVVKIGVFSTSNGFEYFAPANTYNNNIEGQAIVYQSKFLTLNGKKYYFDNNS
                     KAVTGWQTIDSKKYYFNTNTAEAATGWQTIDGKKYYFNTNTAEAATGWQTIDGKKYYF
                     NTNTAIASTGYTIINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTDANNIEGQAILY
                     QNEFLTLNGKKYYFGSDSKAVTGWRIINNKKYYFNPNNAIAAIHLCTINNDKYYFSYD
                     GILQNGYITIERNNFYFDANNESKMVTGVFKGPNGFEYFAPANTHNNNIEGQAIVYQS
                     KFLTLNGKKYYFDNDSKAVTGWQTIDGKKYYFNLNTAEAATGWQTIDGKKYYFNLNTA
                     EAATGWQTIDGKKYYFNTNTFIASTGYTSINGKHFYFNTDGIMQIGVFKGPNGFEYFA
                     PANTDANNIEGQAILYQNKFLTLNGKKYYFGSDSKAVTGLRTIDGKKYYFNTNTAVAV
                     TGWQTINGKKYYFNTNTSIASTGYTIISGKHFYFNTDGIMQIGVFKGPDGFEYFAPAN
                     TDANNIEGQAIRYQNRFLYLHDNIYYFGNNSKAATGWVTIDGNRYYFEPNTAMGANGY
                     KTIDNKNFYFRNGLPQIGVFKGSNGFEYFAPANTDANNIEGQAIRYQNRFLHLLGKIY
                     YFGNNSKAVTGWQTINGKVYYFMPDTAMAAAGGLFEIDGVIYFFGVDGVKAPGIYG"
     gene            complement(17830..18528)
                     /gene="tcdC"
                     /locus_tag="MJE33_002567"
     CDS             complement(17830..18528)
                     /gene="tcdC"
                     /locus_tag="MJE33_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin anti-sigma factor TcdC"
                     /protein_id="HBY9648497.1"
                     /translation="MFSKKNEGNEFSNEGKGSSKKIIKFFKSTKGIALLAFILGVFFG
                     NISSPACSEDHEEVISNQTSVIDSQKTEIETLNSKLSDAEPWFKMKDDEKKAIEAENQ
                     RKAEEAKKAEEAKKAEEQRKKEEEEKKGYDTGITYDQLARTPDDYKYKKVKFEGKVIQ
                     VIEDGDEVQIRLAVSGNYDKVVLCSYKKSITPSRVLEDDYITIRGISAGTITYESTMG
                     GNITIPGIAVEKIN"
     gene            18954..19199
                     /locus_tag="MJE33_002568"
     CDS             18954..19199
                     /locus_tag="MJE33_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pathogenicity locus"
                     /protein_id="HBY9648498.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKLLKDANPAQMYEKECLM
                     RGQHIDRCQLYVYRCAVYFASTENPEPEKLKWWYWKD"
     gene            complement(19417..19686)
                     /locus_tag="MJE33_002569"
     CDS             complement(19417..19686)
                     /locus_tag="MJE33_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBY9648499.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNKGKDQGFDPMVNIEQCRKWLEETVSN"
BASE COUNT         8093 a         2294 c         3086 g         6946 t
ORIGIN      
        1 cgaataaatc atcattataa atataagaga ggatgatttt atgcaaaagt ctttttatga
       61 attaattgtt ttagcaagaa ataactcagt agatgatttg caagaaattt tatttatgtt
      121 taagccatta gtaaaaaaac ttagtagagt tttacattat gaagagggag aaacagattt
      181 aataatattt tttattgaat taataaaaaa tattaaatta agtagctttt cagaaaaaag
      241 cgatgctatt atagtcaaat atattcataa atcattactg aataagactt ttgagttgtc
      301 tagaagatat tctaaaatga agtttaattt tgtagaattt gatgaaaata tcttaaatat
      361 gaaaaataat tatcaaagta agtctgtttt tgaggaagat atttgttttt tcgaatatat
      421 tttgaaagaa ttatctggta ttcaaagaaa agttattttt tataaatatt taaaaggata
      481 ttctgataga gaaatatcag tgaaattaaa aatatctaga caagctgtta ataaggctaa
      541 aaatagagca tttaaaaaaa taaaaaaaga ctatgaaaat tattttaact tgtaattaat
      601 gagcttaaag aaatatttac aatagaaatc aaattttaga attaacttta ttgtaaaatc
      661 aataacttaa tctaagaata tcttaatttt tatattttat atagaacaaa gtttacatat
      721 ttatttcaga caacgtcttt attcaatcga agagcaaatt aatcaactga gtgtcttcaa
      781 tttaaaatgt taggaagtga atgtatatga aaacctaagt agatattagt atattttata
      841 aatagaaagg aggatatata aaagagtttt atcatttaga tgtaaaaata ttatagtaaa
      901 ggagaaaatt ttatgagttt agttaataga aaacagttag aaaaaatggc aaatgtaaga
      961 tttcgtactc aagaagatga atatgttgca atattggatg ctttagaaga atatcataat
     1021 atgtcagaga atactgtagt cgaaaaatat ttaaaattaa aagatataaa tagtttaaca
     1081 gatatttata tagatacata taaaaaatct ggtagaaata aagccttaaa aaaatttaag
     1141 gaatatctag ttacagaagt attagagcta aagaataata atttaactcc agttgagaaa
     1201 aatttacatt ttgtttggat tggaggtcaa ataaatgaca ctgctattaa ttatataaat
     1261 caatggaaag atgtaaatag tgattataat gttaatgttt tttatgatag taatgcattt
     1321 ttgataaaca cattgaaaaa aactgtagta gaatcagcaa taaatgatac acttgaatca
     1381 tttagagaaa acttaaatga ccctagattt gactataata aattcttcag aaaacgtatg
     1441 gaaataattt atgataaaca gaaaaatttc ataaactact ataaagctca aagagaagaa
     1501 aatcctgaac ttataattga tgatattgta aagacatatc tttcaaatga gtattcaaag
     1561 gagatagatg aacttaatac ctatattgaa gaatccttaa ataaaattac acagaatagt
     1621 ggaaatgatg ttagaaactt tgaagaattt aaaaatggag agtcattcaa cttatatgaa
     1681 caagagttgg tagaaaggtg gaatttagct gctgcttctg acatattaag aatatctgca
     1741 ttaaaagaaa ttggtggtat gtatttagat gttgatatgt taccaggaat acaaccagac
     1801 ttatttgagt ctatagagaa acctagttca gtaacagtgg atttttggga aatgacaaag
     1861 ttagaagcta taatgaaata caaagaatat ataccagaat atacctcaga acattttgac
     1921 atgttagacg aagaagttca aagtagtttt gaatctgttc tagcttctaa gtcagataaa
     1981 tcagaaatat tctcatcact tggtgatatg gaggcatcac cactagaagt taaaattgca
     2041 tttaatagta agggtattat aaatcaaggg ctaatttctg tgaaagactc atattgtagc
     2101 aatttaatag taaaacaaat cgagaataga tataaaatat tgaataatag tttaaatcca
     2161 gctattagcg aggataatga ttttaatact acaacgaata cctttattga tagtataatg
     2221 gctgaagcta atgcagataa tggtagattt atgatggaac taggaaagta tttaagagtt
     2281 ggtttcttcc cagatgttaa aactactatt aacttaagtg gccctgaagc atatgcggca
     2341 gcttatcaag atttattaat gtttaaagaa ggcagtatga atatccattt gatagaagct
     2401 gatttaagaa actttgaaat ctctaaaact aatatttctc aatcaactga acaagaaatg
     2461 gctagcttat ggtcatttga cgatgcaaga gctaaagctc aatttgaaga atataaaagg
     2521 aattattttg aaggttctct tggtgaagat gataatcttg atttttctca aaatatagta
     2581 gttgacaagg agtatctttt agaaaaaata tcttcattag caagaagttc agagagagga
     2641 tatatacact atattgttca gttacaagga gataaaatta gttatgaagc agcatgtaac
     2701 ttatttgcaa agactcctta tgatagtgta ctgtttcaga aaaatataga agattcagaa
     2761 attgcatatt attataatcc tggagatggt gaaatacaag aaatagacaa gtataaaatt
     2821 ccaagtataa tttctgatag acctaagatt aaattaacat ttattggtca tggtaaagat
     2881 gaatttaata ctgatatatt tgcaggtttt gatgtagatt cattatccac agaaatagaa
     2941 gcagcaatag atttagctaa agaggatatt tctcctaagt caatagaaat aaatttatta
     3001 ggatgtaata tgtttagcta ctctatcaac gtagaggaga cttatcctgg aaaattatta
     3061 cttaaagtta aagataaaat atcagaatta atgccatcta taagtcaaga ctctattata
     3121 gtaagtgcaa atcaatatga agttagaata aatagtgaag gaagaagaga attattagac
     3181 cattctggtg aatggataaa caaagaagaa agtattataa aggatatttc atcaaaagaa
     3241 tatatatcat ttaatcctaa agaaaataaa attacagtaa aatctaaaaa tttacctgag
     3301 ctatctacat tattacaaga aattagaaat aattctaatt caagtgatat tgaactagaa
     3361 gaaaaagtaa tgttaacaga atgtgagata aatgttattt caaatataga tacgcaaatt
     3421 gttgaggaaa ggattgaaga agctaagaat ttaacttctg actctattaa ttatataaaa
     3481 gatgaattta aactaataga atctatttct gatgcactat gtgacttaaa acaacagaat
     3541 gaattagaag attctcattt tatatctttt gaggacatat cagagactga tgagggattt
     3601 agtataagat ttattaataa agaaactgga gaatctatat ttgtagaaac tgaaaaaaca
     3661 atattctctg aatatgctaa tcatataact gaagagattt ctaagataaa aggtactata
     3721 tttgatactg taaatggtaa gttagtaaaa aaagtaaatt tagatactac acacgaagta
     3781 aatactttaa atgctgcatt ttttatacaa tcattaatag aatataatag ttctaaagaa
     3841 tctcttagta atttaagtgt agcaatgaaa gtccaagttt acgctcaatt atttagtact
     3901 ggtttaaata ctattacaga tgcagccaaa gttgttgaat tagtatcaac tgcattagat
     3961 gaaactatag acttacttcc tacattatct gaaggattac ctataattgc aactattata
     4021 gatggtgtaa gtttaggtgc agcaatcaaa gagctaagtg aaacgagtga cccattatta
     4081 agacaagaaa tagaagctaa gataggtata atggcagtaa atttaacaac agctacaact
     4141 gcaatcatta cttcatcttt ggggatagct agtggattta gtatactttt agttccttta
     4201 gcaggaattt cagcaggtat accaagctta gtaaacaatg aacttgtact tcgagataag
     4261 gcaacaaagg ttgtagatta ttttaaacat gtttcattag ttgaaactga aggagtattt
     4321 actttattag atgataaaat aatgatgcca caagatgatt tagtgatatc agaaatagat
     4381 tttaataata attcaatagt tttaggtaaa tgtgaaatct ggagaatgga aggtggttca
     4441 ggtcatactg taactgatga tatagatcac ttcttttcag caccatcaat aacatataga
     4501 gagccacact tatctatata tgacgtattg gaagtacaaa aagaagaact tgatttgtca
     4561 aaagatttaa tggtattacc taatgctcca aatagagtat ttgcttggga aacaggatgg
     4621 acaccaggtt taagaagctt agaaaatgat ggcacaaaac tgttagaccg tataagagat
     4681 aactatgaag gtgagtttta ttggagatat tttgctttta tagctgatgc tttaataaca
     4741 acattaaaac caagatatga agatactaat ataagaataa atttagatag taatactaga
     4801 agttttatag ttccaataat aactacagaa tatataagag aaaaattatc atattctttc
     4861 tatggttcag gaggaactta tgcattgtct ctttctcaat ataatatggg tataaatata
     4921 gaattaagtg aaagtgatgt ttggattata gatgttgata atgttgtgag agatgtaact
     4981 atagaatctg ataaaattaa aaaaggtgat ttaatagaag gtattttatc tacactaagt
     5041 attgaagaga ataaaattat cttaaatagc catgagatta atttttctgg tgaggtaaat
     5101 ggaagtaatg gatttgtttc tttaacattt tcaattttag aaggaataaa tgcaattata
     5161 gaagttgatt tattatctaa atcatataaa ttacttattt ctggcgaatt aaaaatattg
     5221 atgttaaatt caaatcatat tcaacagaaa atagattata taggattcaa tagcgaatta
     5281 cagaaaaata taccatatag ctttgtagat agtgaaggaa aagagaatgg ttttattaat
     5341 ggttcaacaa aagaaggttt atttgtatct gaattacctg atgtagttct tataagtaag
     5401 gtttatatgg atgatagtaa gccttcattt ggatattata gtaataattt gaaagatgtc
     5461 aaagttataa ctaaagataa tgttaatata ttaacaggtt attatcttaa ggatgatata
     5521 aaaatctctc tttctttgac tctacaagat gaaaaaacta taaagttaaa tagtgtgcat
     5581 ttagatgaaa gtggagtagc tgagattttg aagttcatga atagaaaagg taatacaaat
     5641 acttcagatt ctttaatgag ctttttagaa agtatgaata taaaaagtat tttcgttaat
     5701 ttcttacaat ctaatattaa gtttatatta gatgctaatt ttataataag tggtactact
     5761 tctattggcc aatttgagtt tatttgtgat gaaaatgata atatacaacc atatttcatt
     5821 aagtttaata cactagaaac taattatact ttatatgtag gaaatagaca aaatatgata
     5881 gtggaaccaa attatgattt agatgattct ggagatatat cttcaactgt tatcaatttc
     5941 tctcaaaagt atctttatgg aatagacagt tgtgttaata aagttgtaat ttcaccaaat
     6001 atttatacag atgaaataaa tataacgcct gtatatgaaa caaataatac ttatccagaa
     6061 gttattgtat tagatgcaaa ttatataaat gaaaaaataa atgttaatat caatgatcta
     6121 tctatacgat atgtatggag taatgatggt aatgatttta ttcttatgtc aactagtgaa
     6181 gaaaataagg tgtcacaagt taaaataaga ttcgttaatg tttttaaaga taagactttg
     6241 gcaaataagc tatcttttaa ctttagtgat aaacaagatg tacctgtaag tgaaataatc
     6301 ttatcattta caccttcata ttatgaggat ggattgattg gctatgattt gggtctagtt
     6361 tctttatata atgagaaatt ttatattaat aactttggaa tgatgctatc tggattaata
     6421 tatattaatg attcattata ttattttaaa ccaccagtaa ataatttgat aactggattt
     6481 gtgactgtag gcgatgataa atactacttt aatccaatta atggtggagc tgcttcaatt
     6541 ggagagacaa taattgatga caaaaattat tatttcaacc aaagtggagt gttacaaaca
     6601 ggtgtattta gtacagaaga tggatttaaa tattttgccc cagctaatac acttgatgaa
     6661 aacctagaag gagaagcaat tgattttact ggaaaattaa ttattgacga aaatatttat
     6721 tattttgatg ataattatag aggagctgta gaatggaaag aattagatgg tgaaatgcac
     6781 tattttagcc cagaaacagg taaagctttt aaaggtctaa atcaaatagg tgatgataaa
     6841 tactatttca attctgatgg agttatgcaa aaaggatttg ttagtataaa tgataataaa
     6901 cactattttg atgattctgg tgttatgaaa gtaggttaca ctgaaataga tggcaagcat
     6961 ttctactttg ctgaaaacgg agaaatgcaa ataggagtat ttaatacaga agatggattt
     7021 aaatattttg ctcatcataa tgaagattta ggaaatgaag aaggtgaaga aatctcatat
     7081 tctggtatat taaatttcaa taataaaatt tactattttg atgattcatt tacagctgta
     7141 gttggatgga aagatttaga ggatggttca aagtattatt ttgatgaaga tacagcagaa
     7201 gcatatatag gtttgtcatt aataaatgat ggtcaatatt attttaatga tgatggaatt
     7261 atgcaagttg gatttgtcac tataaatgat aaagtcttct acttctctga ctctggaatt
     7321 atagaatctg gagtacaaaa catagatgac aattatttct atatagatga taatggtata
     7381 gttcaaattg gtgtatttga tacttcagat ggatataaat attttgcacc tgctaatact
     7441 gtaaatgata atatttacgg acaagcagtt gaatatagtg gtttagttag agttggggaa
     7501 gatgtatatt attttggaga aacatataca attgagactg gatggatata tgatatggaa
     7561 aatgaaagtg ataaatatta tttcaatcca gaaactaaaa aagcatgcaa aggtattaat
     7621 ttaattgatg atataaaata ttattttgat gagaagggca taatgagaac gggtcttata
     7681 tcatttgaaa ataataatta ttactttaat gagaatggtg aaatgcaatt tggttatata
     7741 aatatagaag ataagatgtt ctattttggt gaagatggtg tcatgcagat tggagtattt
     7801 aatacaccag atggatttaa atactttgca catcaaaata ctttggatga gaattttgag
     7861 ggagaatcaa taaactatac tggttggtta gatttagatg aaaagagata ttattttaca
     7921 gatgaatata ttgcagcaac tggttcagtt attattgatg gtgaggagta ttattttgat
     7981 cctgatacag ctcaattagt gattagtgaa tagataaaaa tatgttaaat atatcctctt
     8041 atacttaaat atataaaaat aaacaaaatg atacactaca taaagtgttc tatctaatat
     8101 gaagatttac caataaaaag gtggactatg atgaatgcac agtagttcac ctttttatat
     8161 ttctaatggt aacaaaatat ttttttatat aaacctagga ggcgttatga atatgacaat
     8221 atctttttta tcagagcata tatttataaa gttagtaatt ttaactatat catttgatac
     8281 attattagga tgtttaagtg caataaaaag tcgtaaattt aattctagtt ttggaataga
     8341 tggaggaatc agaaaagtag caatgatagc atgtatattt tttttatcag tagttgacat
     8401 tcttacaaag tttaactttt tatttatgtt accacaagat tgtatcaatt ttttaagact
     8461 aaaacatctt ggaatatctg aatttttctc tattttattt attttatatg aaagtgtaag
     8521 tatattaaaa aatatgtgct tatgtggatt accagtacct aagagattaa aggaaaaaat
     8581 agcaatttta ctagatgcaa tgacagatga aatgaatgct aaggatgaaa agtaagtaat
     8641 ggtagatata ataaagatat taacaaataa aaagtgttat ccaaataaga atagctgaaa
     8701 gttatcataa ttcatgaaac taataatgaa aacgagggag cagatgccaa gagacacaca
     8761 agtattaaat acatataatt tcgaagcaag tgttcattac tatatagatg acaaggtagt
     8821 atatcaaaca ttggttcaca aagatggtgc atggtcagtt ggtaaaatct attaagctac
     8881 attagttaca gatatcacaa actataatag ttaaacatag aaatatgtgt aaattgtgat
     8941 ggaaattatt caaaaacaca aaaatacgtg atgaaggaca aaatgatata gaaaataagt
     9001 atcaaacctt aataaatgat ttaattgata gtttaaaagt tataggaaaa atatataaag
     9061 aaataaaaac attaaaaaaa tataagatat gtttacaaat tactatcaga caatctcctt
     9121 atctaataga agagtcaatt aactaattga gtatctttaa attgaaatgt taggaagtga
     9181 tttaaatatg aaaacttaaa ttataaaaaa tcaatattaa tttattttta aaaaatagaa
     9241 aggagtgtat aagatttatt ttcaaagttt aaaaacaaga aaatcaattt aaatttcaga
     9301 aggaataaat gtggttatag aagtggattt attatcaaaa ataataatac taggaggttt
     9361 ttatgtcttt aatatctaaa gaagagttaa taaaactcgc atatagcatt agaccaagag
     9421 aaaatgagta taaaactata ctaactaatt tagacgaata taataagtta actacaaaca
     9481 ataatgaaaa taaatattta caattaaaaa aactaaatga atcaattgat gtttttatga
     9541 ataaatataa aacttcaagc agaaatagag cactctctaa tctaaaaaaa gatatattaa
     9601 aagaagtaat tcttattaaa aattccaata caagccctgt agaaaaaaat ttacattttg
     9661 tatggatagg tggagaagtc agtgatattg ctcttgaata cataaaacaa tgggctgata
     9721 ttaatgcaga atataatatt aaactgtggt atgatagtga agcattctta gtaaatacac
     9781 taaaaaaggc tatagttgaa tcttctacca ctgaagcatt acagctacta gaggaagaga
     9841 ttcaaaatcc tcaatttgat aatatgaaat tttacaaaaa aaggatggaa tttatatatg
     9901 atagacaaaa aaggtttata aattattata aatctcaaat caataaacct acagtaccta
     9961 caatagatga tattataaag tctcatctag tatctgaata taatagagat gaaactgtat
    10021 tagaatcata tagaacaaat tctttgagaa aaataaatag taatcatggg atagatatca
    10081 gggctaatag tttgtttaca gaacaagagt tattaaatat ttatagtcag gagttgttaa
    10141 atcgtggaaa tttagctgca gcatctgaca tagtaagatt attagcccta aaaaattttg
    10201 gcggagtata tttagatgtt gatatgcttc caggtattca ctctgattta tttaaaacaa
    10261 tatctagacc tagctctatt ggactagacc gttgggaaat gataaaatta gaggctatta
    10321 tgaagtataa aaaatatata aataattata catcagaaaa ctttgataaa cttgatcaac
    10381 aattaaaaga taattttaaa ctcattatag aaagtaaaag tgaaaaatct gagatatttt
    10441 ctaaattaga aaatttaaat gtatctgatc ttgaaattaa aatagctttc gctttaggca
    10501 gtgttataaa tcaagccttg atatcaaaac aaggttcata tcttactaac ctagtaatag
    10561 aacaagtaaa aaatagatat caatttttaa accaacacct taacccagcc atagagtctg
    10621 ataataactt cacagatact actaaaattt ttcatgattc attatttaat tcagctaccg
    10681 cagaaaactc tatgttttta acaaaaatag caccatactt acaagtaggt tttatgccag
    10741 aagctcgctc cacaataagt ttaagtggtc caggagctta tgcgtcagct tactatgatt
    10801 tcataaattt acaagaaaat actatagaaa aaactttaaa agcatcagat ttaatagaat
    10861 ttaaattccc agaaaataat ctatctcaat tgacagaaca agaaataaat agtctatgga
    10921 gctttgatca agcaagtgca aaatatcaat ttgagaaata tgtaagagat tatactggtg
    10981 gatctctttc tgaagacaat ggggtagact ttaataaaaa tactgccctc gacaaaaact
    11041 atttattaaa taataaaatt ccatcaaaca atgtagaaga agctggaagt aaaaattatg
    11101 ttcattatat catacagtta caaggagatg atataagtta tgaagcaaca tgcaatttat
    11161 tttctaaaaa tcctaaaaat agtattatta tacaacgaaa tatgaatgaa agtgcaaaaa
    11221 gctacttttt aagtgatgat ggagaatcta ttttagaatt aaataaatat aggatacctg
    11281 aaagattaaa aaataaggaa aaagtaaaag taacctttat tggacatggt aaagatgaat
    11341 tcaacacaag cgaatttgct agattaagtg tagattcact ttccaatgag ataagttcat
    11401 ttttagatac cataaaatta gatatatcac ctaaaaatgt agaagtaaac ttacttggat
    11461 gtaatatgtt tagttatgat tttaatgttg aagaaactta tcctgggaag ttgctattaa
    11521 gtattatgga caaaattact tccactttac ctgatgtaaa taaaaattct attactatag
    11581 gagcaaatca atatgaagta agaattaata gtgagggaag aaaagaactt ctggctcact
    11641 caggtaaatg gataaataaa gaagaagcta ttatgagcga tttatctagt aaagaataca
    11701 ttttttttga ttctatagat aataagctaa aagcaaagtc caagaatatt ccaggattag
    11761 catcaatatc agaagatata aaaacattat tacttgatgc aagtgttagt cctgatacaa
    11821 aatttatttt aaataatctt aagcttaata ttgaatcttc tattggtgat tacatttatt
    11881 atgaaaaatt agagcctgtt aaaaatataa ttcacaattc tatagatgat ttaatagatg
    11941 agttcaatct acttgaaaat gtatctgatg aattatatga attaaaaaaa ttaaataatc
    12001 tagatgagaa gtatttaata tcttttgaag atatatcaaa aaataattca acttactctg
    12061 taagatttat taacaaaagt aatggtgagt cagtttatgt agaaacagaa aaagaaattt
    12121 tttcaaaata tagcgaacat attacaaaag aaataagtac tataaagaat agtataatta
    12181 cagatgttaa tggtaattta ttggataata tacagttaga tcatacttct caagttaata
    12241 cattaaacgc agcattcttt attcaatcat taatagatta tagtagcaat aaagatgtac
    12301 tgaatgattt aagtacctca gttaaggttc aactttatgc tcaactattt agtacaggtt
    12361 taaatactat atatgactct atccaattag taaatttaat atcaaatgca gtaaatgata
    12421 ctataaatgt actacctaca ataacagagg ggatacctat tgtatctact atattagacg
    12481 gaataaactt aggtgcagca attaaggaat tactagacga acatgaccca ttactaaaaa
    12541 aagaattaga agctaaggtg ggtgttttag caataaatat gtcattatct atagctgcaa
    12601 ctgtagcttc aattgttgga ataggtgctg aagttactat tttcttatta cctatagctg
    12661 gtatatctgc aggaatacct tcattagtta ataatgaatt aatattgcat gataaggcaa
    12721 cttcagtggt aaactatttt aatcatttgt ctgaatctaa aaaatatggc cctcttaaaa
    12781 cagaagatga taaaatttta gttcctattg atgatttagt aatatcagaa atagatttta
    12841 ataataattc gataaaacta ggaacatgta atatattagc aatggagggg ggatcaggac
    12901 acacagtgac tggtaatata gatcactttt tctcatctcc atctataagt tctcatattc
    12961 cttcattatc aatttattct gcaataggta tagaaacaga aaatctagat ttttcaaaaa
    13021 aaataatgat gttacctaat gctccttcaa gagtgttttg gtgggaaact ggagcagttc
    13081 caggtttaag atcattggaa aatgacggaa ctagattact tgattcaata agagatttat
    13141 acccaggtaa attttactgg agattctatg cttttttcga ttatgcaata actacattaa
    13201 aaccagttta tgaagacact aatattaaaa ttaaactaga taaagatact agaaacttca
    13261 taatgccaac tataactact aacgaaatta gaaacaaatt atcttattca tttgatggag
    13321 caggaggaac ttactcttta ttattatctt catatccaat atcaacgaat ataaatttat
    13381 ctaaagatga tttatggata tttaatattg ataatgaagt aagagaaata tctatagaaa
    13441 atggtactat taaaaaagga aagttaataa aagatgtttt aagtaaaatt gatataaata
    13501 aaaataaact tattataggc aatcaaacaa tagatttttc aggcgatata gataataaag
    13561 atagatatat attcttgact tgtgagttag atgataaaat tagtttaata atagaaataa
    13621 atcttgttgc aaaatcttat agtttgttat tgtctgggga taaaaattat ttgatatcca
    13681 atttatctaa tactattgag aaaatcaata ctttaggcct agatagtaaa aatatagcgt
    13741 acaattacac tgatgaatct aataataaat attttggagc tatatctaaa acaagtcaaa
    13801 aaagcataat acattataaa aaagacagta aaaatatatt agaattttat aatgacagta
    13861 cattagaatt taacagtaaa gattttattg ctgaagatat aaatgtattt atgaaagatg
    13921 atattaatac tataacagga aaatactatg ttgataataa tactgataaa agtatagatt
    13981 tctctatttc tttagttagt aaaaatcaag taaaagtaaa tggattatat ttaaatgaat
    14041 ccgtatactc atcttacctt gattttgtga aaaattcaga tggacaccat aatacttcta
    14101 attttatgaa tttatttttg gacaatataa gtttctggaa attgtttggg tttgaaaata
    14161 taaattttgt aatcgataaa tactttaccc ttgttggtaa aactaatctt ggatatgtag
    14221 aatttatttg tgacaataat aaaaatatag atatatattt tggtgaatgg aaaacatcgt
    14281 catctaaaag cactatattt agcggaaatg gtagaaatgt tgtagtagag cctatatata
    14341 atcctgatac gggtgaagat atatctactt cactagattt ttcctatgaa cctctctatg
    14401 gaatagatag atatatcaat aaagtattga tagcacctga tttatataca agtttaataa
    14461 atattaatac caattattat tcaaatgagt actaccctga gattatagtt cttaacccaa
    14521 atacattcca caaaaaagta aatataaatt tagatagttc ttcttttgag tataaatggt
    14581 ctacagaagg aagtgacttt attttagtta gatacttaga agaaagtaat aaaaaaatat
    14641 tacaaaaaat aagaatcaaa ggtatcttat ctaatactca atcatttaat aaaatgagta
    14701 tagattttaa agatattaaa aaactatcat taggatatat aatgagtaat tttaaatcat
    14761 ttaattctga aaatgaatta gatagagatc atttaggatt taaaataata gataataaaa
    14821 cttattacta tgatgaagat agtaaattag ttaaaggatt aatcaatata aataattcat
    14881 tattctattt tgatcctata gaatttaact tagtaactgg atggcaaact atcaatggta
    14941 aaaaatatta ttttgatata aatactggag cagctttaac tagttataaa attattaatg
    15001 gtaaacactt ttattttaat aatgatggtg tgatgcagtt gggagtattt aaaggacctg
    15061 atggatttga atattttgca cctgccaata ctcaaaataa taacatagaa ggtcaggcta
    15121 tagtttatca aagtaaattc ttaactttga atggcaaaaa atattatttt gataatgact
    15181 caaaagcagt cactggatgg agaattatta acaatgagaa atattacttt aatcctaata
    15241 atgctattgc tgcagtcgga ttgcaagtaa ttgacaataa taagtattat ttcaatcctg
    15301 acactgctat catctcaaaa ggttggcaga ctgttaatgg tagtagatac tactttgata
    15361 ctgataccgc tattgccttt aatggttata aaactattga tggtaaacac ttttattttg
    15421 atagtgattg tgtagtgaaa ataggtgtgt ttagtacctc taatggattt gaatattttg
    15481 cacctgctaa tacttataat aataacatag aaggtcaggc tatagtttat caaagtaaat
    15541 tcttaacttt gaatggtaaa aaatattact ttgataataa ctcaaaagca gttaccggat
    15601 ggcaaactat tgatagtaaa aaatattact ttaatactaa cactgctgaa gcagctactg
    15661 gatggcaaac tattgatggt aaaaaatatt actttaatac taacactgct gaagcagcta
    15721 ctggatggca aactattgat ggtaaaaaat attactttaa tactaacact gctatagctt
    15781 caactggtta tacaattatt aatggtaaac atttttattt taatactgat ggtattatgc
    15841 agataggagt gtttaaagga cctaatggat ttgaatattt tgcacctgct aatacggatg
    15901 ctaacaacat agaaggtcaa gctatacttt accaaaatga attcttaact ttgaatggta
    15961 aaaaatatta ctttggtagt gactcaaaag cagttactgg atggagaatt attaacaata
    16021 agaaatatta ctttaatcct aataatgcta ttgctgcaat tcatctatgc actataaata
    16081 atgacaagta ttactttagt tatgatggaa ttcttcaaaa tggatatatt actattgaaa
    16141 gaaataattt ctattttgat gctaataatg aatctaaaat ggtaacagga gtatttaaag
    16201 gacctaatgg atttgagtat tttgcacctg ctaatactca caataataac atagaaggtc
    16261 aggctatagt ttatcaaagt aaattcttaa ctttgaatgg caaaaaatat tattttgata
    16321 atgactcaaa agcagttact ggatggcaaa ccattgatgg taaaaaatat tactttaatc
    16381 ttaacactgc tgaagcagct actggatggc aaactattga tggtaaaaaa tattacttta
    16441 atcttaacac tgctgaagca gctactggat ggcaaactat tgatggtaaa aaatattact
    16501 ttaatactaa cactttcata gcctcaactg gttatacaag tattaatggt aaacattttt
    16561 attttaatac tgatggtatt atgcagatag gagtgtttaa aggacctaat ggatttgaat
    16621 actttgcacc tgctaatacg gatgctaaca acatagaagg tcaagctata ctttaccaaa
    16681 ataaattctt aactttgaat ggtaaaaaat attactttgg tagtgactca aaagcagtta
    16741 ccggattgcg aactattgat ggtaaaaaat attactttaa tactaacact gctgttgcag
    16801 ttactggatg gcaaactatt aatggtaaaa aatactactt taatactaac acttctatag
    16861 cttcaactgg ttatacaatt attagtggta aacattttta ttttaatact gatggtatta
    16921 tgcagatagg agtgtttaaa ggacctgatg gatttgaata ctttgcacct gctaatacag
    16981 atgctaacaa tatagaaggt caagctatac gttatcaaaa tagattccta tatttacatg
    17041 acaatatata ttattttggt aataattcaa aagcggctac tggttgggta actattgatg
    17101 gtaatagata ttacttcgag cctaatacag ctatgggtgc gaatggttat aaaactattg
    17161 ataataaaaa tttttacttt agaaatggtt tacctcagat aggagtgttt aaagggtcta
    17221 atggatttga atactttgca cctgctaata cggatgctaa caatatagaa ggtcaagcta
    17281 tacgttatca aaatagattc ctacatttac ttggaaaaat atattacttt ggtaataatt
    17341 caaaagcagt tactggatgg caaactatta atggtaaagt atattacttt atgcctgata
    17401 ctgctatggc tgcagctggt ggacttttcg agattgatgg tgttatatat ttctttggtg
    17461 ttgatggagt aaaagcccct gggatatatg gctaaaatat atgtttgata aaaaattatt
    17521 cctgtgctac taagaaatta tttttatata ataaatattg agatttaatt aaaagtcatg
    17581 tgttattgta atacatgact tttagttaaa atttttctat catttaataa tctattattc
    17641 ttgactattt tataataaaa ttcatatatg gaaatattaa tactaaataa ttaatagttg
    17701 ataaaaaata gataatatgc taaaagcaaa aactaattta gagccttgta actgtttatt
    17761 tgcaattata aaaacatctt taaacatatt gactataata taaaatatta actataatac
    17821 aaaacaatat taattaattt tctctacagc tatccctggt atagttatat ttccacccat
    17881 agttgattca taagttatag ttccagcact tatacctctt atagttatgt aatcatcctc
    17941 taatactctt gaaggagtta ttgatttttt ataactacat agtacaacct tatcataatt
    18001 tccagacaca gctaatctta tttgcacctc atcaccatct tcaataactt gaataacctt
    18061 accttcaaat tttacctttt tgtacttata atcatcaggt gttctagcta attggtcata
    18121 agtaatacca gtatcatatc ctttcttctc ctcttcttct tttttgcgtt gttcttcagc
    18181 ctttttagct tcttcagctt ttttagcttc ttcagcttta cgttgatttt cagcttcaat
    18241 agctttcttt tcgtcgtctt tcattttgaa ccatggttca gcatcagaca atttgctatt
    18301 taaagtttct atttctgttt tttgagaatc tataactgat gtttggttag aaatgacctc
    18361 ctcatggtct tcagaacaag ctggtgagga tatattgcca aaaaacacgc ctaaaataaa
    18421 tgccagtaga gcaataccct ttgtgctctt aaagaattta attattttct tagagcttcc
    18481 ttttccttca ttactaaatt cgttaccctc atttttttta gaaaacataa tacaatctcc
    18541 cttttattag atatatttta ttttaaaagt taaaaactta aaataactag ttgactaaaa
    18601 attatatatt catattttcc taaaatatat ttcccctaag cataatttta caaaataaag
    18661 taagaagatt caatatattt tgaaaaaata agataaaaat gtcagattat aatataattt
    18721 tcttgaaggt gaattttata tattaaatta aacataaagt attttatttg tataaaacta
    18781 tattatgatg taattaagtg actcagtgat ttagaggtgg taaaatatat atatatgaaa
    18841 gagcataaat aaattgatat aataaaaaat taaatattaa atattaacta taataaaaaa
    18901 atatctggta ataggtataa tataaaaaga atataccaga aagaaggtga agtatgggag
    18961 aactacaaaa aattccaggg gtagggaaag caacagaaaa atctttaatc atgttaggat
    19021 atacaactat taaattactt aaagatgcaa acccagcaca aatgtatgaa aaggaatgtt
    19081 taatgcgtgg acagcacata gacagatgtc agttatatgt atacagatgt gcagtatatt
    19141 ttgcatcaac tgaaaatcca gaaccagaaa aattaaagtg gtggtattgg aaagattaaa
    19201 aaatcaaaaa agttagacat aaaaaagagt tgaatatccg ttttaaataa taaaaactaa
    19261 tactcaactc ttttttagtc gtaaatttta tgtaaatcta tttgtatggc taaatatacc
    19321 catcaataaa tgtataaata taccaatcaa caaatgacta aacatactac ctaacaaagg
    19381 attaaataaa ttacttaaca aagcattcta aaacaattaa ttagaaactg tttcttcaag
    19441 ccattttcta cattgctcaa tatttaccat agggtcaaaa ccttggtctt ttcccttatt
    19501 taaaagcata tcacaaggga aagtatcaca ttcaccacaa tgattaagtt ccttactttc
    19561 acagcaagtt ttaaccttac attctccacc ccaaaatggt ttttccatag ttgggcaacc
    19621 agtgcaattt acttgttctt tttttgtaca actgtcacaa cagacaccac atcttgattc
    19681 aaacatgttc aaacatcctt tccattattt aaaaatatta taaaatgatt ttagatgaat
    19741 acaaaagtgc aaatagttga ttttaggtca tctaaatgtt aaacataaaa ttattagtac
    19801 tggtaaattt ataaaaacta ttccaaaaat ctttttgaat atttcaaaac acaataaatt
    19861 acttcaaatc cctttgaata tattttacag cattatagat tgctttaaat ctctttgaat
    19921 attctacaag actatagatt agtttaaatc cctttgaata tattttacaa cattatagat
    19981 tgttttaaat ctctttgaat attctacaag actatagatt agtttaaatc cctttgaata
    20041 tttcaaaaca ccatagatta ctttaaatcc ctttgaatat tctataacac tataaattac
    20101 ttttaacttt tatataacca taaagttttt atctggcact ttaaatggag ttttatatca
    20161 aatttgcctt ccatcgaata accaaactta atttaattac acattccaag agtcaaatat
    20221 tcattcaata tatttgataa ttctatgtta tagctataat taaatataga ttattaaagt
    20281 taaatattta tttaatatat tgaatgattt tttacctata ttcaattaag tttcattata
    20341 taatataaaa attgtttttg cattaagttt taatataata cataataatt ttaaataata
    20401 acattattat atatttttt
//
LOCUS       DAGSRM010000067        20249 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.067, whole genome shotgun sequence.
ACCESSION   DAGSRM010000067 DAGSRM010000000
VERSION     DAGSRM010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20249
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.067"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            69..350
                     /locus_tag="MJE33_002570"
     CDS             69..350
                     /locus_tag="MJE33_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648500.1"
                     /translation="MASKQIPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIENNA
                     LDLFNGKDDVVDGKKVTEILVKAFDYDLTGEVVHLERKNGDIIKVSIVQ"
     gene            614..868
                     /locus_tag="MJE33_002571"
     CDS             614..868
                     /locus_tag="MJE33_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648501.1"
                     /translation="MENKDFKLTICIFAFVIGVVVLFCCIGLGEFSAEHIIQQNGGIM
                     DTDQYNIYLDQSISQYRNFGSILALLGGIGILFDKYIRKY"
     gene            913..1236
                     /locus_tag="MJE33_002572"
     CDS             913..1236
                     /locus_tag="MJE33_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648502.1"
                     /translation="MATEIFTVKITPLEAQKMIKENLDADLVFSDSYNLGDDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTGKTVIKATSTGSAIGILKIDWGAGGNLLKRVKNIL
                     ADYII"
     gene            complement(1795..1989)
                     /locus_tag="MJE33_002573"
     CDS             complement(1795..1989)
                     /locus_tag="MJE33_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBY9648503.1"
                     /translation="MINYMVEIIYSEKISEVENMLKIENIQYEFTSRDGFIFFGFYDM
                     TDAYRFETLCSDEKIRFKRI"
     gene            complement(2363..3982)
                     /locus_tag="MJE33_002574"
     CDS             complement(2363..3982)
                     /locus_tag="MJE33_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBY9648504.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPVLGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLVLNKPISAIVTHFDPTDTARQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDERFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFRNSHMIRLNNGSCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
     gene            complement(4291..4530)
                     /locus_tag="MJE33_002575"
     CDS             complement(4291..4530)
                     /locus_tag="MJE33_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY9648505.1"
                     /translation="MNYNLIKVIFSLFKKKSGYTFTNFLNKVRVEKSKKYILKKDLSI
                     LDVAVLIGFNSQNYYSIIFKKFNNFTPIEYKNIYN"
     gene            4801..4992
                     /locus_tag="MJE33_002576"
     CDS             4801..4992
                     /locus_tag="MJE33_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648506.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTQPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
     gene            complement(5081..5893)
                     /locus_tag="MJE33_002577"
     CDS             complement(5081..5893)
                     /locus_tag="MJE33_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HBY9648507.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNGVTEISDDLDSILTNVVEKIK"
     gene            6315..7466
                     /locus_tag="MJE33_002578"
     CDS             6315..7466
                     /locus_tag="MJE33_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY9648508.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRNHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVVSDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYDGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIAYEIVEFDETNF
                     EKDNDLDKMIELYNQNSTRFIRTRDEFQKLLHAATIAWGPIGYKKVFIKENNNIIGYL
                     IIRTIKKEDSTVGEVAEIGLNSVNVENILKYVANKFGLESLNYKVHVKNLKDQLKCNG
                     TKSLDYQQGTMKIINFTKLCDSLRSYFSQYVDFELLKYMEFKQVENKYIIKYKEEELV
                     IENLDKLNKLFFEKNEEQYNEFKHLKNIYEFATKAFPVDFPWTANLNYQ"
     gene            7584..8222
                     /locus_tag="MJE33_002579"
     CDS             7584..8222
                     /locus_tag="MJE33_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423639.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBY9648509.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            8223..9245
                     /locus_tag="MJE33_002580"
     CDS             8223..9245
                     /locus_tag="MJE33_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HBY9648510.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLDTLRPDYDFPSVLTHTITDRDKLRKL
                     DVPDPYTSGRMPLNIESLSVISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEFIVKNLNKIYNEL
                     DCWKGMHICGDTRELLDNMLSCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRRYDNFLCDFGCNCLNTTPVENLQAAIKAGRISYKELDR
                     IDIDEL"
     gene            9235..9855
                     /locus_tag="MJE33_002581"
     CDS             9235..9855
                     /locus_tag="MJE33_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648511.1"
                     /translation="MNYNSNKNKYLLKDINLDDIFIDKNIVLKFLGYGNRKAPDTISK
                     IVEREIRNINDILDIKIYISEVDINKTPKECKKAFAVLYTIGDKIDFKMNDYMENCNM
                     MAGLALDKVGIVCLDCINEKIKMYLKGKYDSLKISHEIYPGDKDFEVERQKDIYNYVK
                     NKYNSIEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(9979..10665)
                     /locus_tag="MJE33_002582"
     CDS             complement(9979..10665)
                     /locus_tag="MJE33_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBY9648512.1"
                     /translation="MKTYKFIFFDADDTLFDFKKSESHAFKKLLSEFDLEFNFENYIE
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKISLDVDSETLSKMYLNFLGECTFL
                     IPGAIDILQYLKKKYTIVIITNGISAVQKKRLENSKIKEYIDGMVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALIIGDSLTSDILGGINSGIDTCWLNSNNSINYTNHIPTYEI
                     NTLIELKKLL"
     gene            complement(10679..11224)
                     /locus_tag="MJE33_002583"
     CDS             complement(10679..11224)
                     /locus_tag="MJE33_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY9648513.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHDNRKKVILPGSKEVIYEILIPDSS
                     GNLEFAIMDLAPNTSSCVDRITHNGDEIAYVLEGEVKLFMDDDEFTLSKGDSVKVPLG
                     TKHKWQNDSNSESKVIFAVIL"
     gene            11452..12699
                     /locus_tag="MJE33_002584"
     CDS             11452..12699
                     /locus_tag="MJE33_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBY9648514.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVDFMANGAMNISPSILRALASGVLAGSLIKAGAVDKISEQIVKIFGEKRALFSIA
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILLAMLGGGKAGNIISPNPNT
                     IAVADNFSVNLSSVMMANIIPAIIGVVITVILASILINKGNKVQSYEILEQREDLPSL
                     FKSLCGPIIAIFLLFLGNVSPIVIDPMIALPIGGIVTLIVTGNLNNSREYLAFGLSKM
                     QGVCILLLGTGTIAGIIQMSELQQSTIGALQFLNMPQFLLAPVSGILMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIVNAGSSVFEQLPHGSLFHTSAGSINMDIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
     gene            12786..14780
                     /locus_tag="MJE33_002585"
     CDS             12786..14780
                     /locus_tag="MJE33_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBY9648515.1"
                     /translation="MNKKKIVIIGIIYSFLVVFSLTNMYVNMEYNLNVFEYIKKSLPF
                     TEEEKKWLEKHKNLIYSSDQSSPPLRYKGKEDGQYKGIIVDLINSLSIQIGRDFYFKP
                     NNWWKESFVNSIDDSIKFFDLIPSKERANKFIFTDPIYTLSANILKDKKSQDINSYMD
                     LKGKTVAIPEGDYSINFLKQKIQDINILLTPDIKTGVNHLMSGKVDAVVGDEPVLRYY
                     INNYGLSNKYSVLSNPIYTKKAVLAVPKQYEELVSILNKGIFKLQKNGVYKDLKKKWY
                     STYNEVDDILYERGIVPSIYLFIGIILISIYVFYSYTYLLKIEIKRKTEQVIENKKTL
                     EATFNSITDIIMLVDENNNIVESNKVLYDFMGEMSYKIADLISMIKGVIENTFSENTN
                     KTSEIEIHNKILKINTFPVEYKKNNTEYIVVLIKDITNDKIVEAKLLRENKMISIGQL
                     ASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVNDCVKSIESNVDRASNIITNLLNFARI
                     SDDNLEHINIRNFIENIVKLQYKMLQLKNVEIKIDCEHNLVCYINGESLKHVFINLIS
                     NSIDAIHQDGKIIIYCYEKNYCLFIDFKDNGEGIKEDALKDIFNPFYTTKPIGEGTGL
                     GLYITYNEIKKNNGDISVESKLGVGTCFHIKIPLNKEVTI"
     gene            14780..16102
                     /locus_tag="MJE33_002586"
     CDS             14780..16102
                     /locus_tag="MJE33_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBY9648516.1"
                     /translation="MKILLVDDELEYGVVMKKILQKKGYLVDIALSGEEAINIIKKDK
                     NYDLVLSDVMMKNMDGVQLLDRIKSINRDIEVILVTGYGSIENAVDAMKRGALSYFIK
                     SNPIENLLEEVEKVKASKISVSVQKNNLEFTLESKNRDFNNIIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLTTLIEFFLNKYQIEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDKISIRP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKISEYNIK"
     gene            16381..17667
                     /locus_tag="MJE33_002587"
     CDS             16381..17667
                     /locus_tag="MJE33_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBY9648517.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGVLYISSEEIEKIVLEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCRKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            17753..19060
                     /gene="grdB"
                     /locus_tag="MJE33_002588"
     CDS             17753..19060
                     /gene="grdB"
                     /locus_tag="MJE33_002588"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:18800..18802,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HBY9648518.1"
                     /translation="MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQF
                     NDDAEIISTVICGDTYFGENIESATQELLKMIKEESPDLFIAGPAFNAGRYGVACGTI
                     CKAVEEELNIPVITGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVR
                     GEEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTP
                     MKPVKDLSKIKLALVTSGGIVPIDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHG
                     GYDRAYVLENPNLVVPLDVVRELEKEGVIGELVDYFITTTGTGTSVGNSKRFGEEFSK
                     KLLEDDVDAVILTSTUGTCTRCGATMVKEIERAGIAVVHICTVVPISLTIGANRIVPA
                     VGIPYPLGNPMLGEEESKKIRKNIVLKALNALTEDIEEQTVFE"
     gene            19338..20069
                     /locus_tag="MJE33_002589"
     CDS             19338..20069
                     /locus_tag="MJE33_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY9648519.1"
                     /translation="MIRKKVYILISIAICDDESYMLNHLKENIELYMKQKNLLYNIEL
                     FNKVEDLLCNHEKFNIVFLDIQMEEINGMQAARKLRSYGNECFIIFVTVLNEFVFDAF
                     EVDAINYLIKPLSNEKLYKTLDKIVTKLDDNESNYITIQKGYSYWKLKLADILYCEVI
                     KRKIYVHLKKETVDYYETIENLERSLPTNFFRCHRSYIINLQYVYGYENGNAVMESGD
                     RIPVSRLRQQIFSKVMLEFMKNRRI"
BASE COUNT         8015 a         2145 c         3220 g         6869 t
ORIGIN      
        1 catataaaga tatttttaag aataaaattt attattaaat aaaaaataaa ggagagtgat
       61 ttaatagcat ggcatcaaaa caaataccaa gaaacaattt agaaaagttt ttaagaataa
      121 gagtcttatc acatacaggc gataaaatac atataaagtt acctgtaaat tttgcaaaga
      181 gaatgattga gaataatgct ttggatttat ttaatggaaa agatgatgta gtagatggta
      241 aaaaagttac tgaaatatta gttaaggcat ttgactatga tttaactgga gaagtagttc
      301 acttggagag aaaaaatgga gacattatta aagtttcaat tgtacaatag aattatttta
      361 tatgaatttt atttttgaat agttataaat aattagaaat ttgtaaaaaa gtataatata
      421 ttattagtaa tggtacagaa tacacattta taatttatag atgtgtattt tttgtttaaa
      481 aaatttaaat atataaaact ataaaggtta caattttgga aatagatgta aatatatgtt
      541 gtattgtata ataagagtaa tataaaatct agtaaaaata atcaaataaa tttagcttat
      601 gagagggata actatggaaa ataaggattt taaattaaca atatgtatat ttgcttttgt
      661 aattggagta gtagtattat tctgttgtat aggtttaggt gaatttagtg cagagcatat
      721 tattcaacaa aatggtggaa tcatggatac tgaccaatat aatatttatc tagaccaatc
      781 tatttctcaa tatagaaatt ttggttctat attagctctg ttaggaggaa tagggatatt
      841 gtttgataaa tatatcagaa aatactagag attatgtttt gtctaaaata aattcttatg
      901 aggagataaa aaatggcaac agaaatattt actgtaaaaa ttacaccctt agaggcacaa
      961 aaaatgataa aagaaaatct tgatgcagat ttagttttta gtgattctta taatttagga
     1021 gatgataaat ttatactaat tacaactttt gagaagtatt atgcaagaaa taatagtgat
     1081 gcaggattaa tagtagttta tgaaaatacc acaggaaaaa cagttattaa agctacttca
     1141 acaggcagtg caattggaat tttaaaaatt gattggggtg caggtggaaa tttacttaaa
     1201 agagtaaaaa acatacttgc tgattatatt atttaattat tttatctaac tgataatatg
     1261 tcaagaaaat ctcgtaaaag aggttaagaa catttttata atatatagat aaataagaat
     1321 ttgaaggtaa agtttttaag gtataaagaa aaataatata ataaaacgaa ttggctttat
     1381 aattctttat aaggcaaaga gcacatttct ttatataatt gttggatatt aaataatatg
     1441 taaatacacc atttacatat aaaagtttaa ttaatatgtt aagagaaaat gaaatggaaa
     1501 ttactaaaaa atatttttaa attggataat ggttgatatt atgtttatac aaaaatcatg
     1561 tagaatttta tatattttaa atatgatagt ttaaaattat gaaagaatga taaattccag
     1621 tttgtagggt aattttgata aataatatta ataagtatga gtaaaaagta ggagcatatt
     1681 aatataatgt ccctactttt ttattattaa aattgtttat aatatcaaca ctacattaaa
     1741 acacaaccta aatttgaaaa acaaatagtt tagaaatatg tactttttta tctattatat
     1801 tcttttaaat cgaatttttt catctgaaca taaagtctca aatctatatg cgtctgtcat
     1861 atcataaaaa ccaaaaaata taaagccatc acgagaagta aattcatatt gtatattttc
     1921 aattttcaac atgttttcta cttcgcttat tttttcgcta tatataattt ctaccatata
     1981 atttatcatt gtagaatccc tcctattaat atgaaaatat ccaaatatat aataacatat
     2041 ttagatgata taatagtatc caaataaaaa ataatattat tacattattg aatgaagaat
     2101 taaaatgaag ataaataaaa caaatattgg ttataattga ttaaaaaata gttatgtata
     2161 cttgtgactt tatttatggg cagttcaagt tttatgaaag aagtcatata tatatggaat
     2221 attctaggca gaaactgttt aataataaga gaatactata aaaatttgag tattacataa
     2281 aaaataagaa gccataaatg atataagcat tatctatagc ttcttacttc tcattgatta
     2341 agattatata taaaaataat cactatattt tttgtttaag ttttagtact tgttttacta
     2401 tatttgttga gtggtcagaa attctttcta gattagtaag taagtcaaga taaagtacac
     2461 cagcatcaat tgtacaactt ccattattta gtctaatcat atgtgaattt ctaaaagttt
     2521 tttctaaata atttacttca ttttcaactt ctaaaacttt atttgctaac tcaaaatctt
     2581 taacatctaa aactattaag gcatccttaa aattagtaat tgtcttattg tatatttcgt
     2641 atatttctct tatcgcacta tcagaaaaac taatgttcat ttccttaaca ctcatggcca
     2701 attcagctat attttctgca tggtcagaga ctctttcaat gtcatttaca gtattaaaaa
     2761 gcaaatctgt tctaaagcgt tcatcatctc taagaggttc tttagaaagt tttaacaaat
     2821 agttcaatat atctttctgt aacttattga caactttttc tgactcaaaa gctttattaa
     2881 tatcttcatt gctcttattt aaaaatcctg caattgaatg ttccaaagag tttgtagctt
     2941 tatgacccat tcttacaact tcttgtacag tattaccaag tgctatagat ggagtgctaa
     3001 acatcctttc atctaagtac ttagtattat ttactatttc tgattcagtt tctttaatag
     3061 gtataagtct atttgctaat ttaactatgt atttagaaaa tggtaataat attataacat
     3121 ttgtaagatt aaataaagta tgtgcatttg ctatttgtct agctgtatca gttgggtcaa
     3181 aatgagtaac tatggctgat ataggtttat ttaaaacaag cataaatatt attgtaccaa
     3241 ttacattaaa acttaaatgc attacagctg cacgtcgagc atttctactt gcaccaacac
     3301 ttgataaaag tgaagtaaca caagtaccta tattatctcc ataaagaata ggtaaagcag
     3361 atgatagagg aagtattcct tgagaagcta gtgctattaa catacccata gatgcacttg
     3421 aactttgaac aataccagtt atggcaaatc ctgccaatat acctaataca ggatttttac
     3481 taaaatataa aagtgcatca gtgaatgctt tatattcagc aagtggagct actgcatctt
     3541 tcataaactc cattcctgta aataatatac cgaaacctat aagtatctca gataaatgct
     3601 ttgttttttg atttgatgta aataaataga ataaaatacc tatacctaaa gcaattggtg
     3661 caattccttc aagattaaat gaaactaatt gagcagtaac tgtagtacct atatttgccc
     3721 ccattattac accaatagct tgacttagat tcattatacc tgcatttaca aatcctacaa
     3781 ccataacagt agtagcactt gaactttgta taatacctgt aacgactgta cctaccagta
     3841 cacccataac aacattactt gttaagagtt caataatttt ttttaatttg tctcctgctg
     3901 atttttgtaa accttctccc attaaactca ttccgtaaag aaaaagtcca agtccaccga
     3961 taaggctaat tactatattc acgatatttc ctcccttaag aaattaataa tttttttatc
     4021 tgaattataa actttattaa ctaaaattta ttgtttccat aatatagaat aacaaatttt
     4081 gtcaataaaa atattaatgt tttgttaagt atacgttaag agaatgttaa atttttaagt
     4141 tgttgttaat tttagaaaat ttatgatata tagcaattta aatacttgaa cttagatttt
     4201 tagtatttta tgtacaaaca atttaacaaa gaattaacta taattgtaaa aaatatgtaa
     4261 aaaaataaca tccttttaga atgatatttt ttagttataa atatttttat attcaattgg
     4321 tgtaaagtta ttaaactttt taaaaattat actatagtaa ttttgactat tgaatccaat
     4381 tagtacagca acgtctaaaa tagacaagtc tttttttaat atatactttt tgcttttttc
     4441 aactctaact ttatttaaaa aattagtaaa agtatatcca ctttttttct taaacaaaga
     4501 aaaaataact tttattaagt tgtaattcat tacatatatt acttactgtt atatcttctg
     4561 agtaattttt gtgaatataa tttatagttt tctttaagtt ttctttacat aaaactaaga
     4621 gttggtctat gtttcatttt atcaaggcat atttattaaa aaatcaatct ctatttgtta
     4681 aaataattta tgaatgatgt tagaaactgt aatatattgg atataatata ataaacagat
     4741 aaactctgca ttaagaggat atatagttta tcttaattat aggggaagtg aggatatatt
     4801 atggaaagat taatggaact catagaaaga aaatttttaa aggatgtaga acttttagaa
     4861 ttagaagaca tagaaaatgt aatagaatgc aaaatgacac agccatataa tgctcattat
     4921 aatgaatatt tagtaaaagt aaaaaatgga gatgaagtag aagaatattt tgtgtattta
     4981 aaacatagat aaacataaaa gaggtttata tttatttgta tgattataaa ataaatacaa
     5041 gcctcttttt ttgataaata taaaattata aaatttttat ttatttaatt ttttcaacaa
     5101 cattagtcaa tattgaatct aaatcatcac taatttctgt aactccatta tgagtaatat
     5161 ctataatttc tttagtcata ggaagttctc ctaaaagctc taagtctaaa tcatctaaaa
     5221 acttttcagt agattctcct tcaaataact tgattttttt agaacaatca ggacattgga
     5281 tataactcat attttcaata actccaagga catttatgtt cattttttta gccatattaa
     5341 cagcttttga tacaatcata gataccaaat cttgaggtac tgaaaccata actattccac
     5401 ttataggaat agattgcata actgttaagg caacatctcc agtaccagga ggcatatcta
     5461 ttaataaata gtctaattct tcccaaagta catcagtata aaattgcttt acaacccctc
     5521 caagtaaagg tcctctccaa acaacaggtt cattttcatc atctatcata aggtttatag
     5581 acattacttt tatgttattt gagttttcta cagggtaaat ataagagcca tcagaataag
     5641 cttttacatt ttttacaccc ataagtctag gaatacttgg tccagttata tctgagtcta
     5701 atataccaac cttatatccc attttgttta gcttattagc aagtaaagca gttacagttg
     5761 attttccaac tccacccttc ccactcataa caccaattat ttttccaaat ttattgttag
     5821 ggttattttc aatagaacaa tttgattgac tattacaatt tccctttgat ggacatgaat
     5881 tacaatctgc catatctatt acctccaaac tttaatattt attagattat ttgtaaccta
     5941 catatactat tttagtacaa ttgaaccata aagtcattat ttaaaagcaa cttagaagat
     6001 tttctattga tttattcata gactatagca aaaagaatac aataaaaaaa ttataacatt
     6061 cttgaaaaag tgattatttc aatttagtta tagaacttag tcatataaat atatagaaaa
     6121 tattttaata tattatacat aacgggttat tttataagtt taactataaa ttaggtttat
     6181 attttataaa aatttgctaa aaaaaacaat taaaaattgt aaagaaaaaa gatataatta
     6241 gaactgtagt aataaatgtt ttattataga taatagagtt ttaagtatct taaataatat
     6301 ttggaggaat atgaatggaa ttaagaccaa gaagtgcaaa attagaagaa tttgataaag
     6361 taatagaatt aataaactat gtatttagaa tatcaagaaa tcataaacca actatgatgg
     6421 aagaatttcc tcttttacta agtaaaaata atatagaaaa tatgattata atatcagaag
     6481 atgataaagt ggtttctgat gtaaactatc taattcagga tgtatctatt caaggaaaca
     6541 gattgaaggt tgcagctata ggtggagtat gtactcatcc agattatgaa aagagaggct
     6601 attcatctaa gatattggat aaagtagaag aaaaaatgtt ttatgatggt gtagatattg
     6661 taattatatc aggaacaaga agtctataca gtagaagaaa ttgtagtttg gtaaagagtt
     6721 tttataaata tacaataaaa cctgaagatg taaaaatagc atatgaaata gttgaatttg
     6781 atgaaactaa ttttgaaaaa gataatgatt tagataaaat gattgaacta tataatcaaa
     6841 atagtactag atttataaga acgagagatg agtttcaaaa actattgcat gcagcaacta
     6901 ttgcctgggg accaattggt tacaagaagg tatttataaa ggaaaataat aatataatag
     6961 gatatttgat aataagaaca ataaaaaaag aagattctac tgtaggagaa gttgctgaaa
     7021 tagggttaaa ctctgtaaat gttgaaaata tattgaaata tgttgcaaat aaatttggat
     7081 tagaatcttt aaattataaa gtacatgtga aaaatcttaa agaccagttg aaatgtaatg
     7141 ggacaaaatc tttggattat caacaaggaa ctatgaaaat aataaatttt actaagttgt
     7201 gtgatagttt aagaagttat tttagtcaat atgtagattt tgaattacta aaatacatgg
     7261 aatttaaaca agttgaaaat aaatatatta ttaaatacaa agaagaagaa cttgtaatag
     7321 aaaatttgga taagcttaat aaactgtttt ttgaaaaaaa tgaggaacag tacaatgaat
     7381 ttaaacatct aaaaaatata tatgaatttg ctacaaaggc atttcctgta gattttccat
     7441 ggactgcaaa tttaaattat caatagtttt agatgatatt aaataaaatt gattgatacc
     7501 tagagttagt ccttgttaaa taagagaaat tattggatat tgaaaaagca attttgtaag
     7561 taattagaaa ggatgagtat tatatggaca tacttgatga tatagcagaa tgtatattaa
     7621 atatgggtgt agataatata gagaatttag taaaaattgc tatagataaa aatattgatg
     7681 tggaagatat ttatgaatat ggtttaaaca aaggtatgat aggagcttta gataagtttg
     7741 agaataagga atactatctt tcagaagtta tagtatgcac agatgcatta aataaaggta
     7801 ttaatctttt gaagggaact ggaaaagtaa agaaaaaatc aaagggagta attctgatgt
     7861 ctgtagtaga aggagatact catgagatag gaaaaaatat tgtaaaagta atggtagaag
     7921 ccactggata taaggttatt gacttaggtg taaatagaaa aagtgaagat ataatagaag
     7981 aagctataaa aaacaatgta gatataatag gcttatcttc aatgatgaca actacaatgg
     8041 aaaatatgaa atcggttata gacgaactta atatgattga gatagataaa agaccaaaag
     8101 taataattgg tggaggacct gtaagtatgg agtttgcaga agaaatagga gcagatggat
     8161 acagttcaaa tgctccaaaa gcagttaaat taataaataa acttatagga ggtgaagttt
     8221 aaatgttttc agatatgact ttacttcagt atataaatag tgaagataga ggtttttttc
     8281 tgcctgatat gggtacaaat ggattgttct taataaataa aaaggcatat gaagtatatg
     8341 atagtgtaga tttgcaatta gaattagcaa agactatgga caagtatttt gaatcagatt
     8401 ttatatactc attctgtgat gggattacat tttgtgaaac tctgggttta gatacattaa
     8461 gaccagatta tgattttcca agtgtattga cacatactat aactgacaga gataaattaa
     8521 gaaaattaga tgttccagac ccttatacga gtggacgtat gccactcaat atagagagct
     8581 tatcagttat atcaaaaagt attaaaaaac cactatatga atcgatacaa ggtccattta
     8641 cacttgcagg tcaattagct ggagctactc aacttttaag atgtataata actgataaaa
     8701 aatttgtaga agaattattg gaatttacaa ctgaattggt aagaagatat gctgtagctg
     8761 caaataaagc aggagcaaaa tatatttcaa tagcagagcc aacatcagta accctaagta
     8821 aagatagatt tgatgaattt atagtaaaaa atttaaataa aatctacaat gaacttgatt
     8881 gttggaaagg tatgcatata tgtggagaca ccagagaatt attagataat atgctaagtt
     8941 gtaatataga tgcagtaagt ttagaccaaa ttttagacta tgaagaaatt gctccaatga
     9001 ttcctagaga tatagttttg attggtaatt tagacccaat aaaacttctt ggacgttcta
     9061 agccagataa gattagaaga gagacactaa aattgttaaa gaagatgaga agatatgata
     9121 actttttatg tgattttggc tgtaactgct taaataccac tccagtagaa aatttacaag
     9181 cagctataaa ggcaggaaga attagttata aagaattaga taggattgat attgatgaat
     9241 tataatagta ataaaaataa atatctttta aaggatataa atttagatga tatttttata
     9301 gataagaata ttgtgctaaa gtttttgggg tatggaaata gaaaagctcc agatacaatt
     9361 agtaaaatcg ttgaaagaga aataagaaat ataaatgata ttttagatat caagatatat
     9421 ataagtgaag ttgatattaa taaaacacct aaagaatgta aaaaagcatt tgctgtacta
     9481 tatacaatag gggataaaat tgattttaag atgaatgact atatggaaaa ttgcaatatg
     9541 atggcagggc tggcattgga taaggtaggt atagtatgtt tagattgtat taatgagaaa
     9601 ataaaaatgt atttaaaagg aaagtatgat agtctcaaaa tatcgcatga aatatatcca
     9661 ggagataaag attttgaagt agagagacaa aaggatattt ataactatgt aaaaaataaa
     9721 tataatagta tagaaataga aattaatgat tatcatcaac tatcccccat aaaaagtgtt
     9781 gcaatgctta ttttaatggg ggatgaagaa aatttagaat caagatgtag taagtgtcct
     9841 agaaaatgct tttagttaat tttcaagact aagatttgtt ttgtgtataa attgcaataa
     9901 tttttttggt cattttatgt ataaactaaa aaaattattg caaagtaata ttctatttaa
     9961 tttgaacaga cttacaaact atagtagttt ctttaattca ataagtgtat ttatttcata
    10021 agttggtata tgattagtat aatttatact gttattggaa tttaaccaac aagtatctat
    10081 tcctgaattt attccaccta agatatcaga ggttaagcta tccccaatta ttaaggcaga
    10141 agatttatca tgacaattaa actttttgag agcgtattta aatatttcag gatttggttt
    10201 tgatattttc aactcttcag atacaaccat tccatcaata tattctttta tttttgaatt
    10261 ttcaagtctt ttcttttgaa ctgcagaaat tccatttgtt attataacaa tagtatactt
    10321 ttttttaaga tattgtaata tatcgattgc acctggaatt aaaaatgtac attcacctaa
    10381 aaaatttaag tacattttgc taagagtttc agaatcaaca tccaaagata ttttatttgc
    10441 aaaaagttca aatctaagta atttaagttc attcaaagtt ataatatttt tttcaaggtc
    10501 gagccaaagc ttatcactta tatttctata tgactctata taattctcaa aattaaattc
    10561 taaatcaaac tcagatagaa gttttttaaa agcatgtgat tcacttttct taaaatcaaa
    10621 taaagtatca tctgcatcaa aaaatataaa tttatatgtc ttcatggttt ttactccttt
    10681 acaagatgac tgcaaaaatt actttacttt cactatttga gtcattttgc catttatgtt
    10741 ttgttcctaa aggaactttt acactatctc ctttggacaa agtaaattca tcatcatcca
    10801 taaaaagttt aacttctccc tccaaaacat atgcaatttc atctccatta tgagtaatcc
    10861 tatcaacaca agaagaagtg tttggagcta aatccataat tgcaaattct aaattaccac
    10921 tagagtctgg aattaaaatc tcatatataa cttctttgct tccaggtaaa ataacttttt
    10981 ttctattatc atgtcttact atcaagtttt ttttatctac ctcagataca aaaaatgtaa
    11041 ataaaggaac gtttagagaa gaggctatag attttaatga atttacagat ggattagcaa
    11101 gacctcgttc tatttggctc aataatgatg gtgtaacatc agctagtttt gctaaatctc
    11161 gaatacttaa gttttgtttt tttcgaactt cagctatttt ttcccctaaa tttaaatcat
    11221 tcatataaat atcctctctt tttaatttaa aaaatattaa cttaaaataa tcaagtaaaa
    11281 tgattatgta gtatgaaatt attataacat atagaattag gttttgaaaa aactatgttc
    11341 aatataatta aataaattaa aatataattg acaaagtgga tacagaatac ttaaaatata
    11401 tttaaataaa attaagcaaa agatagttaa tttatgatag gaggggatat tatgcaaata
    11461 acgacttttg gagctatatt tggtttatta atagcaatta ttttaattat aaaaaaattt
    11521 caagctgtat atagtttaat gctaggagca tttattggag gattagttgg aggagcaaat
    11581 ataacacaaa ctgtagattt tatggcaaat ggagctatga atatatcacc atcaattctt
    11641 agagctttgg catcaggtgt attagctgga agtttaataa aagcaggggc agtagataaa
    11701 atatcagaac agattgtaaa aatatttggt gaaaagaggg ctttattttc gattgccatt
    11761 tcaactatgg tacttgctgg agtaggcgta aatttggatg tatctataat aactgtagca
    11821 cctataggat tatatatagg tagaaaatta aattattcta aattgtcaat actattagct
    11881 atgttgggag ggggtaaagc aggtaatata atatcaccaa atccaaatac tatagcagta
    11941 gcagataatt tttctgtcaa tctctctagt gttatgatgg caaatataat tccagcaatt
    12001 attggagttg ttattacagt tattttagca agtatattaa taaataaagg aaataaagta
    12061 caatcatatg aaattttaga gcaaagagaa gatttaccaa gtttatttaa atcattatgt
    12121 ggtccaatta tagctatatt tctattgttt ttaggaaatg tatctcctat agttatagac
    12181 ccaatgatag cccttccaat aggaggaatc gtaactctaa ttgtcacagg aaacttgaac
    12241 aattcaagag aatatttagc ttttggatta tctaagatgc aaggggtatg catattatta
    12301 cttggaacag ggactatcgc aggaattata cagatgtctg aattacaaca gagtacaata
    12361 ggtgctttac aatttttaaa tatgccacaa tttttattgg caccagtttc gggtatactt
    12421 atgtcattag caacagcatc atcaacagct ggtgctacta tagcatcatc tacttttcat
    12481 gatgctataa taaatggtgg gctatcaccg atttcaggtg cctcaatagt aaatgctggg
    12541 tctagtgtat tcgagcaatt accacatggc tcactatttc atactagtgc aggtagtata
    12601 aacatggata taggtgaaag atttaagtta attccatatg aagcattgat aggaatagta
    12661 atgactataa tttcaacatc tattcaatta gttttataat aaatataaat atttaatgaa
    12721 aaaagtagga ttttgttatt aaatattgaa tttattatag taaaaaagaa gattggagtt
    12781 ttaaagtgaa taaaaaaaag attgttatta tagggattat ttattcattt ttagtagtat
    12841 tttcacttac aaatatgtat gtaaatatgg agtataatct aaatgtattt gaatatatta
    12901 agaagtcact tccatttaca gaagaagaaa aaaagtggtt agaaaaacac aagaatttga
    12961 tttacagctc tgaccaaagt tcacctcctt tgagatataa aggtaaggaa gatggacagt
    13021 ataagggaat tattgttgat ttaataaatt ctctgtcaat tcaaattggt agagattttt
    13081 attttaaacc aaataattgg tggaaagaat ctttcgtaaa ttcaatagat gatagtataa
    13141 agttttttga cttgattcca tcaaaagaaa gggcaaataa atttatattt actgacccaa
    13201 tatacacttt gagtgcaaat atattgaaag ataaaaagtc ccaagatata aatagttaca
    13261 tggatttaaa ggggaaaaca gtagcaattc cagaaggtga ttattctata aattttttaa
    13321 agcaaaaaat tcaagatata aacattttgt tgactccaga tataaagact ggagtcaacc
    13381 atttaatgtc tggaaaagta gacgcagttg ttggtgatga accagtattg cgatactata
    13441 taaataatta tggactttca aataaataca gtgttttaag taatcctata tatactaaaa
    13501 aagcagttct tgctgtacct aaacaatatg aagagctggt tagtatttta aataaaggca
    13561 tttttaaact tcagaaaaat ggagtgtata aagatttaaa gaaaaaatgg tattctacgt
    13621 ataatgaagt tgatgatata ttgtatgagc gaggaattgt gcctagtatt tatcttttta
    13681 taggtataat tttaatatca atttatgttt tttatagcta tacatatttg ttaaagatag
    13741 aaatcaaaag gaaaacagaa caggtaatag aaaataaaaa aactttagag gcaactttta
    13801 atagtattac agatataata atgcttgtag atgaaaataa caatatagta gaatcgaaca
    13861 aagtactata tgactttatg ggtgaaatga gttataagat agcagatttg attagcatga
    13921 taaaaggtgt tattgaaaat accttctctg aaaatactaa taaaactagt gaaatagaaa
    13981 tacacaataa aattttaaag ataaacactt ttcctgtaga gtataaaaaa aataatactg
    14041 agtatatagt agttcttatt aaagatataa ctaatgataa aatagtagag gcgaaattac
    14101 ttagagaaaa caaaatgata tctataggac aattagcttc tggggtagca catgagataa
    14161 gaaatccttt gggtataata agaaataatt gctatctctt aaaagataac gttacaatgg
    14221 aagaagtaaa tgattgtgtt aaatcaatag aaagtaatgt agatagggca agtaatataa
    14281 taaccaactt gttaaatttc gcaagaatat cagatgataa tttagaacat ataaatataa
    14341 gaaattttat tgaaaatata gtgaaacttc aatataaaat gttacagtta aagaatgttg
    14401 aaataaaaat tgactgtgaa cataatttag tatgctatat aaatggagaa tcgttaaaac
    14461 atgtatttat aaatttaatt tctaattcaa tagatgcaat acatcaagat ggaaaaatta
    14521 ttatatactg ttatgaaaaa aattattgtt tatttataga ttttaaagat aatggtgaag
    14581 gaataaaaga agatgcccta aaggacatat ttaatccctt ctatacaaca aaaccaattg
    14641 gtgagggaac tggtttaggt ttatacatta cttataatga gattaaaaag aataatggag
    14701 atataagtgt tgaaagtaaa ttgggggttg gaacttgttt tcatataaaa attccattaa
    14761 ataaagaggt gactatataa tgaaaatact attagttgat gatgagctag aatatggagt
    14821 ggtaatgaaa aaaattctac aaaagaaggg ctatcttgta gatatagcac taagtggtga
    14881 agaagctata aatataatta aaaaagataa aaattatgat ttggttttgt ctgatgttat
    14941 gatgaaaaat atggatggag tacagctatt agatagaatt aaatctatta atagagatat
    15001 agaggttata ttagttacag gatatggaag tattgaaaat gcagttgatg ctatgaaaag
    15061 aggagcttta tcttatttta taaaaagtaa tcccattgaa aatttattag aagaagttga
    15121 aaaggtaaag gcaagtaaaa tatctgtttc tgtacaaaaa aataatctgg aatttacttt
    15181 ggaatctaaa aatagagatt ttaataatat cataaaaata gcaaaaaaag ctgcttgtaa
    15241 agatgttaat atattaatct tgggggaatc tggtgtagga aaagatatat tagctagata
    15301 tatacatagc ataagtccaa gaaagaatga gatatttgta cctgtaaatt gttgttcttt
    15361 ttcagaaaat ctattggagt ctgagttgtt tggtcatgaa aaaggttctt ttacaggagc
    15421 agtagatagt agaaaaggta gatttgaact ttcgaataaa ggaactctat ttttggatga
    15481 aataggagat atacctctaa atgttcaggt aaaacttttg aggacacttg aggacaaatc
    15541 tatagagagg ataggaagta ataaatctat taaagttgac tttaggttaa tttgtgctat
    15601 gaataaagaa ccaaaagtgg aaatatcaaa tggaaatata agagaagatt ttttttatag
    15661 aataagtaca ataacaataa ctataccacc gctaagaaag agaagagaag acttaactac
    15721 tcttatagaa tttttcttaa ataagtacca aattgaacat gataaaaaga tacactcgat
    15781 tgataaggaa gtaaaggatt ttttattaaa ttacaattat ccaggaaata taagagaatt
    15841 aaaaaatata ataaatagac ttgtagtttt aagtgaagaa ggcaatttat caaaagacaa
    15901 tttaaattta atatcaaata atgtatacat agatgataaa ataagtatta gacctttaag
    15961 agaaataaga aaagagtttg aatgtgagta tattgaaaaa gttttatctt tgtgtggaaa
    16021 taatatttca aatacagcta aaaaattgga gattagtaga agacagttaa ccaataaaat
    16081 atcagagtat aatattaagt gagaaaaaaa cttctcatta agtgagaaaa aaatttctca
    16141 aactgtatta aaagataaac agaaaaaatc tgtttatctt tttttattat aattttaaaa
    16201 attaatattt acaataatta ttgctaaatt caatattggt gtaaattaaa aatgaaaatt
    16261 tctatgacat taatacatag aatatatatt ttaaataatt ggcatagaaa atgctttata
    16321 tattaataaa cgtaataaat acttttaatt gcgattaaat atattaaggg ggattttcat
    16381 atgaagctag agctagggaa tatatttata aaaaatgttg aatttggaga aaagacagaa
    16441 gtaaaagatg gagtactata tattagtagc gaagaaatag aaaagatagt attagaagat
    16501 gaaaggattg taagtgtaaa tgttgaactt gcaagacctg gcgaatcaat aagaatagct
    16561 ccagttaaag atgtaataga gcctagagtt aaaattggag atgaaagcaa aatatttcct
    16621 ggaattataa ataaagtgaa gactgtagga agtggaagaa cccatgtact tttaggagct
    16681 tgtgttgtaa cttgtggaaa tatagttgga tttcaagaag gcgtaattga tatgagtggt
    16741 cctactgcta aatatacacc tttttcaaaa acaaataata tatgtattgt tataaaagca
    16801 aaggatggta tagatactca tgattatgaa gaagcagcta gaattgcagg attaaaaata
    16861 ggtgcatatg taggtgaggc aggaagagaa gttgagccag atgaagtcat tgtatatgag
    16921 acaaagccat tattaaaaca agtaaaagaa tatccagacc tgccaacagt agcatatgtt
    16981 cacatgttac aatcacaagg acttttacat gacacttatt actatggggt ggatgctaaa
    17041 cagatagtgc ctacatttat gtatccaact gaaataatgg atggagctat aatatctgga
    17101 aactgtgtag caccatgtga taaggttaca acattccatc atttaaataa tccagttata
    17161 catgacttat ataaaagaca tggaaaagat ttaaacttta taggagttat tttgactaat
    17221 gagaacgtat tcttagtcga taaagagaga agctctgata tggtagctaa attaatagaa
    17281 tttttaggag ttgatggagt tctagtgaca gaagaaggtt atgggaatcc agatactgac
    17341 ctaatgatga attgtagaaa atgtagtgaa gttggagcaa atgtagtttt gataacggat
    17401 gaatttcctg gaaaagatgg caagagtcaa tctatagcag acgctactaa ggaagcagat
    17461 gcagttgttt cttgtggtca aggaaatcta gtggttcatt tcccagcaat ggaaaaaatt
    17521 ataggaactt tagattatgt tgaaatgatg ataggtgggt ataaaggatg cttaaatgaa
    17581 gacggaagca tggatgcaga gctacaaatt ataatagcat caactatagc aaatggctat
    17641 aatcatttga cagcaagata ttattaaata agtatttgaa tataaagttc tgttgatata
    17701 atcattggtc taattgatgt aacaactata aagtgatttg gaggattaag agatgagtaa
    17761 aatcagagtg gttcattata taaacaattt ctttgctggt ataggtggag aggaaaaagc
    17821 agatattccg ccagaaaaaa gggctggtgt agtaggacct ggaatagcat ttcagagtca
    17881 atttaatgat gatgcagaga taatctcaac tgtaatttgc ggagatacgt attttggtga
    17941 aaatattgag agtgcaactc aagaactttt aaaaatgata aaagaagaaa gccctgattt
    18001 atttattgca ggcccagcat ttaatgcagg tagatatgga gtagcttgtg ggactatttg
    18061 taaggcagtt gaagaagagt taaatattcc tgtaattaca ggcatgtata aagaaaatcc
    18121 aggtgtagat atgtttaaat tagatttgca tatagtgtct acaggaaatt cagcagcaag
    18181 tcttagaaag gtagttccaa taatgacaaa actaggtctt aaattggtta gaggtgagga
    18241 aataggacct cctgaagagg aaggatatat aatgagagga ataagaaaaa atttcttcca
    18301 tgaacttaga gggtctgaaa gagcaattga tatgttggtt aaaaagatga aaggggaaag
    18361 ctttgaaact gagtatccaa tgcctgaatt tgatagagtt acacctatga aacctgttaa
    18421 agatttatct aagataaaac ttgccctagt aacttctgga gggatagttc caattgataa
    18481 tccagacaaa attgaatctt caagtgctac taaatatgga atttacgatt taacagatat
    18541 ggattctatg tcaaataaag aatttacaac tatacatggg gggtatgaca gagcatatgt
    18601 ccttgaaaat ccaaatttag ttgtaccact agatgtagtt agagagcttg aaaaagaagg
    18661 tgtaattgga gaattagtag attattttat aactactaca ggaacaggaa cgagtgttgg
    18721 caattctaag agatttggag aagagttttc taaaaaattg ttagaagatg atgtagatgc
    18781 tgtaatttta acttctacat gaggtacttg tactcgttgt ggagcaacga tggtaaaaga
    18841 gattgagaga gctggtatag cagttgttca tatatgtaca gtagtaccaa tatcacttac
    18901 aataggagct aatagaattg ttccagcagt agggatacca tatccacttg gaaatcctat
    18961 gttaggagaa gaggaaagta aaaaaattag aaagaatata gttttaaaag ctttaaatgc
    19021 actaacagaa gatatagaag aacaaacagt atttgaataa taaaaataca acacaacact
    19081 gaaataaaaa aagatatcaa aaaataattt tttaaaatta ttttacgata tctttttttt
    19141 attaggtaaa ttcgaaattt gcatttttta aaattaaatt tataaaagtg ataaattagt
    19201 ttattataat attgatacac aaattcaaat tagatatact atgaaaagcg agattattac
    19261 attttggaac aaatattcaa catattagat catattaatt gaacagaaaa aattaacttt
    19321 atataataat tagaataatg ataagaaaga aggtgtatat attgatttca attgccattt
    19381 gtgatgatga atcctatatg ctaaatcatt taaaggaaaa tatagaatta tacatgaagc
    19441 agaagaattt attatataat atagagcttt ttaataaagt agaagattta ctatgcaatc
    19501 atgaaaagtt taatattgta tttttagata tacagatgga agagattaac ggaatgcagg
    19561 cagctagaaa attaaggtca tatggtaatg agtgctttat aattttcgtt acagttttaa
    19621 atgaatttgt atttgatgct tttgaagtag atgctataaa ttatctgata aaacctctat
    19681 ctaatgagaa actatataaa actttggata aaatagttac aaaacttgat gataatgaaa
    19741 gtaattatat cacaatacaa aaaggttatt catattggaa attaaaactt gctgatattt
    19801 tatattgtga agttataaaa agaaagattt atgttcattt aaagaaagag actgtagatt
    19861 attatgagac tattgaaaat ttagagagaa gtttaccaac aaattttttt aggtgtcatc
    19921 gtagttatat aatcaattta caatatgtat atggatatga aaatggtaat gcagttatgg
    19981 aaagtggaga tagaatacct gtttctagac tacgtcaaca aatattttca aaagttatgc
    20041 ttgagttcat gaaaaatagg aggatataag atgattaatt tattttcttc cgatttattt
    20101 ttaaagtaca gttttttatt ttcttttatt gtatatagaa ttttaatggt aatagtgcaa
    20161 tactattttc taatcagttt tacaaatgtt aattcaaaat tttggtgttt tattttatat
    20221 tcgataattt caattttttt aatcattat
//
LOCUS       DAGSRM010000068        20213 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.068, whole genome shotgun sequence.
ACCESSION   DAGSRM010000068 DAGSRM010000000
VERSION     DAGSRM010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20213
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.068"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            278..1471
                     /gene="nifV"
                     /locus_tag="MJE33_002590"
     CDS             278..1471
                     /gene="nifV"
                     /locus_tag="MJE33_002590"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HBY9648520.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVV
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            1496..3421
                     /locus_tag="MJE33_002591"
     CDS             1496..3421
                     /locus_tag="MJE33_002591"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HBY9648521.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREDAWVELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSDVEVVRGP
                     NIKPFPLNTELSQSIGGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            3443..4438
                     /locus_tag="MJE33_002592"
     CDS             3443..4438
                     /locus_tag="MJE33_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HBY9648522.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGVEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            4764..5237
                     /locus_tag="MJE33_002593"
     CDS             4764..5237
                     /locus_tag="MJE33_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBY9648523.1"
                     /translation="MVVSVEVCLIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKG
                     QFTFLTRICENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDEFDKRGYNLT
                     PTDKSLEVYELIIEEENRSIEICFDNFTDEEKQVVTKLLEKMSKNVENEWFKVKR"
     gene            5271..5765
                     /locus_tag="MJE33_002594"
     CDS             5271..5765
                     /locus_tag="MJE33_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY9648524.1"
                     /translation="MIRKANMNDLESIMKIIKSTVEEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNPNFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            5862..6383
                     /locus_tag="MJE33_002595"
     CDS             5862..6383
                     /locus_tag="MJE33_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HBY9648525.1"
                     /translation="MIGFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSKGLRTWE
                     FIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAASYIQLQ
                     AHELGLGSCWIQVRNRMHDDNIEADKYIREELKVPSKYSVECIISIGYPDEEKKAYSD
                     SDLDYKKVHFNNF"
     gene            6572..6883
                     /locus_tag="MJE33_002596"
     CDS             6572..6883
                     /locus_tag="MJE33_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBY9648526.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            6911..7597
                     /locus_tag="MJE33_002597"
     CDS             6911..7597
                     /locus_tag="MJE33_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBY9648527.1"
                     /translation="MKYSYKKCIIDSILLMFIVQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLSLA
                     IIVTYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKSKIICITSIL
                     SGIYNIGYIDVIRNYVILYNNSSYTFEVIISKIMIGTVFGIVLGLVKYRFRDVGFCIL
                     LRSLFAIFIR"
     gene            7829..8713
                     /locus_tag="MJE33_002598"
     CDS             7829..8713
                     /locus_tag="MJE33_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBY9648528.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNNFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPYIVHKEEFEHPLKLDISSV
                     VNGEVRQNSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
     gene            8812..9987
                     /locus_tag="MJE33_002599"
     CDS             8812..9987
                     /locus_tag="MJE33_002599"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HBY9648529.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEDLLKDIPTDNKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            10115..10969
                     /locus_tag="MJE33_002600"
     CDS             10115..10969
                     /locus_tag="MJE33_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648530.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSTESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFDEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKEGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKVKDDSSC
                     LYLDIYND"
     gene            11013..12248
                     /locus_tag="MJE33_002601"
     CDS             11013..12248
                     /locus_tag="MJE33_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBY9648531.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREMVKDIKVMSICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKTFGVSESKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKAISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYISLPFMEKEGIPKDEELYDFENNLYNAIDSKIQSLLNNSNKKTSVDMSKKTW
                     DGLAYRINEVILMDELCLV"
     gene            12349..13290
                     /locus_tag="MJE33_002602"
     CDS             12349..13290
                     /locus_tag="MJE33_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HBY9648532.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVV
                     LVSNKSDKSILSGASKVTAIGISDKSIIEQCLDAVKK"
     gene            complement(13341..13700)
                     /locus_tag="MJE33_002603"
     CDS             complement(13341..13700)
                     /locus_tag="MJE33_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648533.1"
                     /translation="MDLFEVNIVLNSGTAGGMNPKLKIFNMVISTEVCHHDVTQDTLT
                     EFHSWLKTVFFEANCELIKISEMAVKKTNQEETVFWGRILAGEAFITNKGRKKINEKE
                     AYLHLIASSHIFYTIIK"
     gene            complement(13726..14190)
                     /locus_tag="MJE33_002604"
     CDS             complement(13726..14190)
                     /locus_tag="MJE33_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY9648534.1"
                     /translation="MNLGNSLFQARKKHGLSQEDVAEKLGVSRQTVSKWETDETVPDI
                     RQSKKLAILYNVSLDELIDFDIDMEEIQEAINKTSEETEEKINWTNAWGKKYPILLKY
                     QTDVNISNYAYKVNMMLDELKQEYQYNELEAMLILKDILYNVWKNRKSKRKQ"
     gene            complement(14358..14566)
                     /locus_tag="MJE33_002605"
                     /pseudo
     CDS             complement(14358..14566)
                     /locus_tag="MJE33_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004071281.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
     gene            complement(14610..14963)
                     /locus_tag="MJE33_002606"
     CDS             complement(14610..14963)
                     /locus_tag="MJE33_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBY9648535.1"
                     /translation="MDRKRELKQLYKEMKFDTGVFIIKNDITKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFTFEVLELIKHDDNKTEKDYLEELDILEMVW
                     LEKLKEDNTYEIYSI"
     gene            15259..16170
                     /locus_tag="MJE33_002607"
     CDS             15259..16170
                     /locus_tag="MJE33_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HBY9648536.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYKITEEDELYIVPTEDAVFTMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEILEFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLVFSKFYTEQLDWIVSFAKDMCKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
     gene            16431..16865
                     /locus_tag="MJE33_002608"
     CDS             16431..16865
                     /locus_tag="MJE33_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648537.1"
                     /translation="MISPIILSSINQNLKEIERNELLETNIESGDYGLALSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLEAINDMQEIFYYLKNETD
                     DKICDDEIIEILGDFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            16888..18423
                     /locus_tag="MJE33_002609"
     CDS             16888..18423
                     /locus_tag="MJE33_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648538.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIDEREVYNIQQ
                     EISLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYKLMMSRKLDTNSIAYNDTLMELSSFFKSYIVKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGVTYK
                     IDCDVILVNLFELIMNNCIFSTILGNEVTNLEISKYEYEFLEKQFKNMYNNITEDKVI
                     DSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTFKSMVTVSKNKNEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFMLNEKTETTLWKIEYIKFMKRQSEDKIYSIEEHMN
                     KIN"
     gene            18507..19874
                     /locus_tag="MJE33_002610"
     CDS             18507..19874
                     /locus_tag="MJE33_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBY9648539.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTVMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKAKDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFVGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMDTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            19909..20133
                     /locus_tag="MJE33_002611"
     CDS             19909..20133
                     /locus_tag="MJE33_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HBY9648540.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGATMTIKAIIENVLVSEVPGVTQVLGV"
BASE COUNT         7945 a         2041 c         3720 g         6507 t
ORIGIN      
        1 gttttttgaa aataaatcta tttaaaaatt aaattaaaat gcaagaaaaa cataataact
       61 ttcattttta agaagttttc acaaaaaaga aaaaatatag tgcaataaat gatacaaagt
      121 aatataatag attacaagat atgaaaagct aaaaaaataa attgcaaatg aaattaaaat
      181 gaataaaaaa taaaaaaata atcataagtt agaggtatca atgaaaaata agacttaatg
      241 tttttaaaat taaaagtata ctaagggggg cattaatatg tgcgttatat caaaagatag
      301 agcaaaggaa ataaaaatag tagatactac tttaagagat ggagagcaaa cagctggggt
      361 tgtatttgca aatagagaaa aaataatgat tgctgaaatg ttaagtgacc taggtgttga
      421 ccaaattgaa gttggtatac ctactatggg tggagatgaa aaaaatgtaa taaagcacat
      481 atgtagtaga aatttaaaat cagatataat ggcttggaat agagcagtaa taaaagatgt
      541 tgaagaatct atttcttgtg gtgttgatgc agtagctata tcaatatcag tatctgacat
      601 tcatatagaa aataaattga gaacttctag aggctgggtt ttagaaaata tggctaaaac
      661 agttgaattt gcaaagaaaa atggattata tgtttcagta aatggagaag atgcttcaag
      721 agcagatata gacttcttaa ctgaatttat aaatgttgga aaacaagcag gggcagatag
      781 atttagatac tgtgatacag taggagttat gaatcctttt tctataaaga atgcaataga
      841 aactctttat gaaagaacta attttgatat agaaatgcat acacataatg actttgggat
      901 ggcaactgca aatgcacttg caggaatagc agcaggagca aattatgttg gtgttacagt
      961 aaatggatta ggtgaaagag caggaaatgc agcattagaa gaagttttaa tggcacttaa
     1021 gtgtgtttat aaatgtgatt taaataatat agatactaga aaatttagag gaatatgtga
     1081 gtatgtagca caggcatcag gaagaatact tcctacatgg aaaccagtag ttggggataa
     1141 tatgtttata catgaatctg gtatacatgc agatggagct ttaaaagacc cacataacta
     1201 tgaaccattt gacccaagtg aagtaaatct tgaaagaaaa atagtaatag gtaaacattc
     1261 gggaagagct gcagttgtaa ataagttgag tgaatatgaa atgtatattt ctccagaaaa
     1321 cgctaccaag ctattaaatg ctataagagc tacatctata agattaaaaa gaagtttaat
     1381 ggataaagaa attcttcaat tatattgtga tatactagca catgagaaag gcacaacaga
     1441 agaagaagca gttagaggtt catatattta agatataatt gtttaggagg aaaatatggg
     1501 agataatata gtttataaaa taattaaaaa acatatagta gatggagaag ctgttgctgg
     1561 aagttcaatt ggtataaaaa tagaccaaac tttaacacaa gattctactg gaacaatgac
     1621 atatcttcaa ctagaagcta tgggaataga taaagtaaag actaaaagaa gtgtggcatt
     1681 tgtagaccat aatatgttac aacaaggatt tgaaaatgca gacgaccata agtatataca
     1741 aacagtggca gataaatatg gagtttattt ctctaagcca ggaaatggta tttgtcatca
     1801 agtattttta gaaagatttt caacaccagg agatacactt ttaggttctg atagccacac
     1861 acctactgct ggaggtgtgg gcatgatggc tattggagct ggtggattag atgttgcttt
     1921 agccatggca ggaggagcat actatataaa agcaccaaaa gtctgtaagg tgaatctagt
     1981 tggaaaatta aataatatgg tatcatcaaa agatataata ttagaagtgt taagaaaaca
     2041 aactgtaaaa ggtggagtag gtaaagttta tgaatatggt ggagaaggtg tcaaaagcct
     2101 atctgttcca caaagagcta caataacaaa tatgggggct gaacttggag caactacttc
     2161 aatcttccca agtgatgaaa aaacactaga attttttaag agtcaaggaa gagaagacgc
     2221 atgggtagag ctaaaaccag atgcagatgc agtttatgat gaagagatta ctataaactt
     2281 ggatgaatta aaaccattag ctgcaaaacc tcatagccca gacaatgtag atgaagttga
     2341 aaatataggc aagattaaaa tagaccaagt ggcaatagga agttgtacaa attcttctta
     2401 tgaagactta atgaaagtag cacaaatcct gaaaggaaat aaagtacata aggatgtgag
     2461 tttggtaata gcaccaggtt caagacaagt tatggagatg attgctagaa atggggcttt
     2521 ggcagatata ataagtgctg gagctagaat actagaaaat tcttgtggtc catgtattgg
     2581 tatgggtcaa tctccaggta ctgactctgt gtctttaaga actttcaata gaaatttcta
     2641 tggtagaagt gggactttgt cagcacaagt atatttagta agtccagaag tggcagcagt
     2701 aagtgcaatc aagggagtgc ttacagaccc aagagaattt gatattaaat ttacaaattt
     2761 agatgtaaat gaatttttga tagatgatag catgataatt aaacctgctg atgtaggaag
     2821 tgatgtagaa gtagttagag gacctaatat aaaacctttc cctcttaata cagagctaag
     2881 tcagtctata ggtggaaaag ttatattaaa gacagaagat aatataacta ctgaccatat
     2941 aatgccatca aatgctaagt tacttccttt tagaagtaat ataccgtatc ttgcaaacta
     3001 ttgtttcaat actgtagata cagaatttcc tcagagagca aaagacaata atggtggatt
     3061 tatagttggt ggagataatt atggtcaagg ttcaagtaga gaacatgcag ctttagcacc
     3121 attatatcta ggtgttaaag gggtaatagt taagagcttt gcaagaatcc acaaggctaa
     3181 cttgataaat agtggtataa ttccaatgga gttttgtgat gagaaagatt atgaaaatat
     3241 atctctttta gataatctgg agataccaaa tattttagac aatctaggta gtggaatttt
     3301 agaagttaag aatacaacta aaggaacttc ttttaaggtc aaagtagaat tgtcagcaaa
     3361 agaagttgat gtattaaaag ctggtggaaa attaaactac actaaaaatc aagctaatta
     3421 atatgaaaag agggaatatt gaatgtataa agtgacactt ataccaggag atggaatagg
     3481 accagaagtt gcgaaagcta tgaaaaaggt agtagaagct actggagtag aaatagaatg
     3541 ggaagaagtt aatgcaggag aggcagttat tgaagagtat ggtactccac ttcctgaata
     3601 tattatagat agtataaaaa agaacaaaat agctataaaa ggcccaatta ctactcctgt
     3661 aggtaaagga tttaggagtg taaatgtaac tttaagacaa gcattagatt tgtatgtcaa
     3721 tttaagacct attaaaagtt ttaaggggat aaaatcaaga tatgaagatg ttgatttagt
     3781 ggttgtaaga gaaaatacag aagatttata tgctggaata gaacataaaa taggtgatta
     3841 tgcagctgaa agtataaaga taataacaag aagtgcaagt gaaagaatcg ttgattttgc
     3901 atgtaactat gttaaagata ataagagaaa aaaagttaca gctatacata aggcaaatat
     3961 aatgaaaatg tctgatggat tgtttttaga tgtgtttaga gaagttgcat caaaacatgg
     4021 tgtagaatat gatgatttga ttgtagatgc agcagctatg aacttagttt taaatccaga
     4081 aaattatgat gtaatggtaa tgccaaatct ttatggagat atactatcag atttaggtgc
     4141 tgggcttgtt ggaggtttag gtataattcc aagtgcaaat attggtaaag attgtgcgat
     4201 atttgaagct gttcatggaa gtgctccaca aatagcaggt caaaataagg ctaatccaac
     4261 agcacttatt caatcatcag taatgatgct tagatactta ggtgaatatg aaaatgcaca
     4321 aaaaatagaa acagctttag aaaaagtatt tcttgaagga agtaaattga ctgtagattt
     4381 aggtggctca gcttctacta cagagtttgc ggatgaggtg tgtaagtata ttgtatagat
     4441 tttatttagg gaaaaagtaa aatatagatt gaagaaaaga ggctttctca aaatagagat
     4501 aagccttttt tattggaatg ataaaatata tttgatttta tacaaaagag agttcctcat
     4561 aaacgaaaaa gttaattttg ggatatccat tttaatttta aaattaattg attaatatgc
     4621 aaagaaagat taaataaatt tattattttt aattgaactt tcaaccaaaa tatggtaaaa
     4681 tttaaaaaag ttgaactttc aactaaaaat gaatatattg attatgtgaa attctataaa
     4741 acagaaacta tttgacttta gccatagtag tttcagtgga ggtgtgtttg attaagactt
     4801 tagacagtaa tatattaaga gaagtaggta ctttatctag agcagtaaat tcaataaatg
     4861 atattaagta caaggaatta aagttacaaa aaggacaatt tacatttttg acaagaatct
     4921 gtgaaaatcc tggtataaat cttgtggaat tatcaaatat gttaaaggta gacaaggcta
     4981 caacaaccaa ggctatacaa aaattaataa aggcaggata tgtagataaa aagcaggatg
     5041 agtttgataa aagaggatat aatttaactc caactgataa atctttggaa gtttatgagt
     5101 tgataattga agaagagaat agaagtatag agatatgttt tgataatttt acagatgaag
     5161 aaaaacaagt ggtaactaag ttattagaaa aaatgagcaa aaacgtagaa aatgaatggt
     5221 ttaaagtaaa gagatagtaa ataaatacat taacttaagg agatattttt atgattagaa
     5281 aagcgaatat gaatgattta gaaagcatta tgaaaattat aaaatctaca gtggaagaaa
     5341 tgaagactta taataataca caatgggatg aaaactatcc attagaaaaa gactttgttt
     5401 cagatattaa gaaacaagat ttatatattt atgaagtgga tggagaagta gcagggttta
     5461 tatgtcttaa ttatgaagag ccagaagaat atttaggctt aaattggtca tctaataaaa
     5521 aagcaatggt aatacataga atggctgtaa atccaaactt tagaaagaca gggatagcat
     5581 ctaagttagt tgattttgct gaaaaattag ctgtagaaaa caatgtatca tacttaaaga
     5641 gcgatacata ttcaataaac tcaaaaatga atttattact tacaaagtgt ggttttataa
     5701 aaatcggaga aatgagtttt ttaggaaagg agaaatcttt ctactgttat gataaaatat
     5761 tataaagagc atttacaata caaggggtaa ataatagatt ttcagcatta gtaagggcat
     5821 aatataactt agtatatgct attatgaaag ggatgattta tatgattgga tttctaaaga
     5881 aaagaagaag tattagaaaa tacaaggatg ttgaagtaga aaaagaaaaa ttagataaaa
     5941 tattaaaagc tgctctttta gctccatcat caaaggggct tagaacatgg gaatttatag
     6001 tggtagatga taaagaaaag ctaattaatt tatctcaatg tagaactaaa ggtggtggat
     6061 ttttcttaaa aaatgcacct ttagctattg taattattgc agataaagaa aaaaatgatg
     6121 tttggataga ggatgcaagt atagctgctt catacataca acttcaagct catgaactag
     6181 gtttaggttc ttgttggata caagtaagaa atagaatgca tgatgataat atagaagctg
     6241 ataagtacat tagagaagaa ttaaaagtac ctagcaagta cagtgtagaa tgtataattt
     6301 ctataggata tcctgatgaa gaaaagaaag catacagtga ctctgattta gactataaaa
     6361 aggttcattt taacaacttt tagtatataa tatgttgtat taagacatta tatttttttc
     6421 tattgttaat atatacttaa aattgtaaaa atatatctga attttactaa cttatctagg
     6481 ggttatgtac aaggatttta aagataaata tagaaattaa tgtttataat cgatacataa
     6541 cttgagtaac aaaatttaag gaggttaaag tatgggtata tttaaaacaa aaatggatga
     6601 ggactggaaa gtaaactaca ttaaagagtt taatgaaatg agagattctt atgagagtaa
     6661 acttcaaaaa aaacagtttg aggtagatag tttgaaatca gaattggata gactaagaag
     6721 ctataaaaat agccttaagc caaaagaaaa acaaattact gatgatgata taaataatat
     6781 aaagagttta agaagagatg gcttaagcta taaagaaatt tcaaatcaaa caagttggag
     6841 caaagcaact gtaagtaggg tattaaatgg actttacgat tagcatatac atggggtgat
     6901 ttaaattggg atgaagtata gttacaaaaa atgcataata gattctattt tattgatgtt
     6961 tatagttcaa atattaagga tgattttgaa ttatgtactt ctaagtcaat ttgaatttac
     7021 tcttgaaaat tttaatatta taaacttaat ttcttttaca ttagttggtt tatcgttaat
     7081 tttattttta aaagataatt ctttgtataa caaggttaga aatagaaaaa tcacagaagc
     7141 ttttgaagaa aataaaaaca atatacttat tgaaaagtgt aagctgatac tttttgttgt
     7201 ggttttatct ttagcaataa ttgtaactta ttgtactaag ggatatgtac tttttaatgt
     7261 aactatgatg actttgtctg tattgatagt tccaatattt gaagagttat tttttagaga
     7321 atatatatgg aactatctaa gtaattttat aaagtcaaag agtaaaatta tctgtataac
     7381 atctatacta tctggaattt ataatatagg atacatagat gttataagaa attatgtaat
     7441 attgtataac aattcgagct acacctttga agttattatt tcaaaaatta tgataggtac
     7501 tgtttttggt attgtacttg gattagttaa gtataggttt agagatgtag ggttttgtat
     7561 attattgagg agcttatttg ctatatttat taggtagata gtataatcgg tttataaggt
     7621 tgttttaaat ttaattttaa atcaacctta ttttatttgt gttttataaa gcacttatat
     7681 attaataact aataaattta ataaaaatta tttgaatatt tttaaaatat ataaatatgt
     7741 attgacatat aaatattatg aattacaatt tatataaact aataaaaatt aaataatatt
     7801 aatcaactta ttgaatggag gaataaaaat gaaatttgtg actttttgta gtagcaatga
     7861 agaaaaaata ggtgtattta attcagaaac aaatagtata tatgaaatta atagtttagg
     7921 attaagtaaa ttatatactg atatgaataa ttttattgaa aacgtatcta caggagattt
     7981 agaaaaaata aaaaacaatt catttgaaaa cgcaaagtgt tataagttag aagaagttaa
     8041 attatgttcc ccaattgtaa gacctaaaaa agatattata tgtttaggtc taaactataa
     8101 agaccatgtc aatgaaatcc cagatggagt tataaaaaac gtagttatgc cagattatcc
     8161 aatttatttt tctaagagag cagacaaaat aattggagtg gatgataaga taagcttaca
     8221 tggagatttg gtagagaagt tggactatga atcagaactt gcagtaatca taggtaaaga
     8281 aggtataaat atatcaaaag aagatgctta tgaatatata tttggatata caatagtaaa
     8341 tgatataagt gaaagagcac ttcaagataa acatgttcaa tggtttagag gaaaaagctt
     8401 agacacacat acatctatgg gaccttatat agtacataaa gaagaatttg aacatccttt
     8461 aaaattagat ataagtagtg tagtaaatgg agaagtaaga caaaattcta atacagagta
     8521 ctttatattt gatataccta ctataataaa tgatttatca agaggaatga ctctaaagcc
     8581 aggagatata atttcaactg gaactccagc aggggttgct atggggatga atcctcaagt
     8641 atatttaaag catggcgata tagttgaatg taaagtcgaa ggaataggag ttttaaaaaa
     8701 tatagttgat taatggataa atatgccata taattttatt agtacgaata atttaattaa
     8761 tataggatta atcaaactta tgattaattt attatagaga gggatgagta tatggcaaga
     8821 gaactagaat taaagtatgg ctgtaatcca aatcaaaaac catcaaagat ttatatgaag
     8881 aatggagaac ttcctataga agtattaaat ggaaaacctg gatatataaa ttttttagat
     8941 gcatttaata gttggcaact tgtgaaggaa ttaaaagaag ctacaggtct cccagcagca
     9001 acttcattta aacatgtaag tccagcagga gcagcagtag gggtaccttt atcagatact
     9061 ttaaagcaaa tatattttgt tgatgattta gagctttctc cattagcttg tgcttatgca
     9121 atggcaagag gagcagatag aatgtcttct tatggcgatt ttatagcact ttcagatgta
     9181 tgtgataagg agactgcaac tattatagca agagaggttt ctgatggtat aatagctcca
     9241 ggatatacag atgaagccct tgaaatatta aaaggaaagc gtaaaggcaa ctataatatt
     9301 gtaaaaatag atgaaaatta tactcctgaa cctattgaaa caaaggatgt ttatggaatt
     9361 acttttgaac aaggtagaaa tgaaatatta ataaatgaag acctattaaa agatattcca
     9421 actgataata aaatttttac agatagtgca aaacgtgatt taattatagc tcttattaca
     9481 ttaaaatata cacaatcaaa ttctgtttgt tatgctaaag atggacaagt aataggggta
     9541 ggtgcaggtc aacaatcacg tattcattgt actagacttg caggtaacaa agctgatact
     9601 tggtatcttc gtcaacatcc taaagtatta aacttaaaat ttaaaaagga tataggtcgt
     9661 ccagatagag ataacactat tgatgtatat ctttctgatg attatatgga tgtgcttgct
     9721 gatggcatat ggcaaaactt ttttgaagaa aaaccagagc ctctaactgg agaaggaaaa
     9781 agagcatggc ttaaaacttt aacaggagta gcacttggtt cagatgcatt tttcccattt
     9841 ggagataata tagaacgtgc aaaaagaagt ggggtaagtt ttatagcaca accaggtggt
     9901 tctattcgtg atgataatgt tatattaact tgtaataagt ataacatagt catggcattt
     9961 actaaaaata gattattcca tcattaaaat actgaaagta taaagataat atagattagg
    10021 taaaaatcta caacaagttt tgttgtagat ttttttattt gctgtataat tttaaataga
    10081 actttatttt tagatattca atttggaggt tgatatggct ttaaatataa gaaactatag
    10141 cctattatgt aatcaaggaa cagctaaatt taatgaagat gtagtcggta taaatccatt
    10201 tggggcatgg gttttagatg gtgctacagg acttaatgga aaaaatttag tgtcaactga
    10261 aagtgacgca aggtggtatg ttcagtggtg gaataggtat ttatataaaa atataaataa
    10321 agaagaatca ttaaagagta ttattaatac tggaataata gaagtaaaaa aagagtatga
    10381 actaagatta aatgggttta aggttgaaaa attagattta ccatctagtt ctatagttgt
    10441 tgtaaaattt catgaaaata aagtagagta ttttttatta ggagattgtg ctttgtttat
    10501 taaaaatgga gattccaaag ttataaaaga taggagtatt tgtaaatttg ataatactgt
    10561 atttgatgaa atgagtaaac tttctaactt ggaagagatg gcttttgatg agattagaac
    10621 tagtgttatg gatacaatag taagcaatag attgaagaaa aatacaaaag agggctattg
    10681 gatattggat tttgatgagg aagctgtgga aaattctata tatggataca ttgaaataaa
    10741 aaaagatttt caattgatgc taacgagtga tggttttaca agtgcatgtg atagatataa
    10801 tctaataaaa gaagaagaat tgatacgtat agcagaaaaa tttggtatag gatatataca
    10861 taataaagta agggattttg aagataatga ctataaagcg gttaaagtgc caagatttaa
    10921 agtaaaagat gattcatctt gtttatattt agatatatat aatgattaga ctgtataaaa
    10981 ctatttaatt ttagaaagga ggcaagtgaa aagtgaaaat tcttcatata ataacacaaa
    11041 aaccaaatag tacaggtagt ggtatatatt tgtctggtat gataaaaggg ttcgaaaaaa
    11101 tcggtcataa gcaagctgta attgcaggta tagatgtaaa tgatgatgta aattgttttc
    11161 caagtgaagt aagtttttat ccagtaaagt ataattgtgg ggagttaaat tttcctgttg
    11221 taggtatgag tgattctatg ccatatgaaa gtacaagata taaagattta aatatagaca
    11281 tgataaatag attgaaatat caatttaaag taaatataga taaggctatg aatgatttta
    11341 aaccagaact tataatatgt catcatttgt acctcttaac tgcatttgtt agggaaatgg
    11401 taaaagatat aaaggttatg tctatttgtc atggaacttg tttaagacag ttaaatacca
    11461 ttgatttaga gaaagaatac ataatagcaa atataaggaa attagattta atatttgctc
    11521 ttcatgaaaa tcagaaatat gatattataa agacttttgg agtaagtgaa agtaaagtag
    11581 ttgtaattgg tagtggttat aatgatgata tattttataa taaaaattat aaaataaagg
    11641 atgataaaat aaaaatagta tttgcaggta agatatgtaa atcaaaggga ctgattccat
    11701 ttataaaagc tatatctaaa ttaaaatata gtaaagattt aatagaagtc aattttgcag
    11761 gtactggaag cgatatcgaa tcatataatg agatagttaa actggcaagt aagtctccat
    11821 ttaaaatgaa tttcctagga aagttagaac agagagattt agctgagcta ttcaacaggt
    11881 cacaaatatt tgtgttacca tcattttatg agggattacc agttgtagtt ttagaagctt
    11941 tatcttgtgg cacagatgta atcactactg atattcttgg tgttaaagag tggattggta
    12001 gtgagattaa taattctgga aagatagaat atatttctct accttttatg gagaaagaag
    12061 gtattccaaa agatgaagag ttatatgatt ttgaaaataa tttgtataat gcaatagatt
    12121 ctaagataca atctttattg aataactcaa ataagaaaac atctgtagat atgtcaaaaa
    12181 aaacgtggga tggcttagca tatagaataa atgaagttat tttaatggat gaattgtgtt
    12241 tagtctaaaa ttatttaaat tataaaaaag tgtaactatt tttacactat ttttaaatga
    12301 ttttatatta tactaatact aaatatattt gttttaggga ggtaatttat gaggattagt
    12361 aaaaaggtat tggcgttagg tatttcagca ttactgttaa gtgttagttt tccaacatca
    12421 ataaacgcac tagataagat tgaaaatatt caaggagtag ataaatatga aacagctggg
    12481 cttatttcag ataagcaaga ttttacaaca gcagttctta tcaatgcaga ttctacaatg
    12541 gtagatggaa ttgctgcaag tggtcttgca ggagtaaata atgcagctat tttattaact
    12601 aataaggatg atataccaga agcaacgctt caaagactga atagagttac taagatatat
    12661 gtaataggtg gagaaaattc tataagtaaa gatgtagaaa aaatgttact tatgagaaga
    12721 atgcaagtta taagaattga tggtgtagat agagtagata caagttataa aattgcaggc
    12781 gaaatagaaa aaattaaaaa cagtgataag atgtttttag taaatggatt taaggatgaa
    12841 gctgatgcag taagtgttgc atctgttgct tatagagatg gagctcctat aatattgaca
    12901 aaagatatac cttcagcaga agaagacaca gacttagacc catggtttgg agtttctcct
    12961 gttccagtat atgctattgg aggagagtct acattaagtg attacatagt gagcagatat
    13021 agagcaacta gaattggcgg tgttgataga tatcaaacta ataaaaatgt tattgaaaag
    13081 ttctataatg gagcaaaaga gttctatata acaagtggag acgatttagt ttatgcttta
    13141 gttgcatcac cacttgcaaa aaatgctcct gttgttttag tttcaaataa aagtgataag
    13201 agtatattaa gtggagctag taaagtaaca gcaataggta taagtgataa gagtattata
    13261 gagcaatgtt tggatgctgt aaagaagtaa tggagtgtta ttagtaaaat gaatatacac
    13321 attgaaaatt agtagattgt tcattttata atggtataaa atatatggga agaggctatc
    13381 aaatggagat aagcctcttt ttcatttatt tttttgcgtc ccttattggt aataaaagct
    13441 tcccctgcta aaattcttcc ccaaaatacg gtttcttctt ggttggtttt ttttacagcc
    13501 atttcagata tttttatcaa ttcacagttt gcttcaaaaa atacggtttt caaccatgag
    13561 tgaaattctg tcagagtatc ttgtgtaaca tcatgatgac aaacttccgt agatattacc
    13621 atattaaata ttttcaattt tgggttcatt ccacctgctg tccctgaatt gagaacgata
    13681 tttacttcaa aaaggtcaat actttagttt ccaaaaaacg gggatttatt gctttcgttt
    13741 actttttcga tttttccaga cattgtataa aatatcttta agaattaaca ttgcttccag
    13801 ttcattatat tggtattcct gttttaattc gtccaacatc atgttaacct tataagcata
    13861 attagaaata ttaacatctg tctgatattt aagaagaata gggtattttt ttccccaggc
    13921 atttgtccaa ttgatttttt cttcggtttc ctcacttgtt ttattaatcg cttcctgaat
    13981 ttcttccatg tcaatatcaa aatcaatcaa ttcatctaat gacacattgt aaagtattgc
    14041 taattttttt gactgacgaa tgtctgggac tgtttcatcc gtttcccatt ttgaaacagt
    14101 ctgcctgctt acccccagtt tttcagctac atcttcctgt gaaagcccat gtttcttcct
    14161 tgcctgaaat aaactatttc ctaaattcat tgaaattcct ccttatctgt ttatgggatt
    14221 attatattat ttgataagaa caaactctac caactaaaat tatcatattt gctattttta
    14281 ttaacataat tcaaagacgt atttattctg caaattaaaa tctgctaacc tctgcgaata
    14341 tcaatcatta accactttta tccattccct gtttttgttt gtacgtaaat atctcttaat
    14401 tctggtgtaa actcctgtaa aatcgcttcg acaacctctt tctctttctt tcaaacaaca
    14461 actaaaacgt aaaataattt tctttcggat atactgtgca taaatttttt ctaggttttt
    14521 taaatttgat attccagcca aattcccaac tgcaagaact aaatatctat tttgagacac
    14581 tatctccttt tcttatatta tattattttt tatatggaat aaatttcata agtattatct
    14641 tcctttaatt tttctaacca aaccatttct aaaatatcta gttcttctaa atagtctttc
    14701 tctgttttgt tatcatcatg ttttataagc tctaaaactt caaatgtaaa tgctttttca
    14761 ccatattttt tccattcatt atttaattct tttatcatac atgagccagc accaagctga
    14821 aatttgagag agttaaattt gctttttata tcattacttt ttccaaggta aatcttttta
    14881 gtaatatcat tttttattat gaatacacct gtgtcaaatt tcatttcttt gtataattgt
    14941 tttaattcac gttttctatc catgatttta gatacctctc tttataattg atatgtaata
    15001 tgtaagtgta aaaatatatt acatatcaat tataaagaga ttaattttat atgtaaatag
    15061 tttttataaa tttaattatg aattagtcat caataaacaa gaaatttagc tgtagaaatc
    15121 cattataaag gctattataa attttaatat gctaaaaata taaaaagaat agttgacagt
    15181 gtagaaaata tggaataaac taaaagtgta atagtaatat taaattatat attacattga
    15241 aaggtgtgaa tgattattat gaataaaaaa atagaattaa tagggaattt tgcaagtgat
    15301 tggattaggt atagtgatta tgaatataaa attactgaag aagatgaatt atatatagtt
    15361 ccaacagaag atgctgtgtt taccatgtat aacccatttg atgtagcaga tgatataata
    15421 gtagatataa ttcgcatagg acaagaggca ttacaagata tatcaaaaga gacaaaagaa
    15481 atgaaactaa agaaagaaat acttgaattt gtaaaaaagt atggattatt gggtttaata
    15541 tattcaagtg tatataatag agatatagtt ggagaagaaa gagtactgat gattgaaaag
    15601 aattatatta caaaagagaa tatattgagt acagatgaat atgtaaacat gtttatacca
    15661 tttgcaagtg atgaagatgt gtattttaaa aagtatagaa ggggtataga tgtatcaaag
    15721 agagaagaat ctccaaagtt ttttggaaag agaccattga ttttagattt agtattttca
    15781 aagttctaca ctgaacaact agattggatt gtaagttttg ctaaagatat gtgtaagcat
    15841 tttaatcaaa tttgtgtata taaaaataca tctaatctat taactgaaag tgttactatt
    15901 atgtctggag ggtttaatcc tcaaaagata ggattttcta taagccaatt agataaacca
    15961 attattgcat gggagtttga ttctttgaaa acagctattg agactatcta tgcctttgca
    16021 attacaagtg aaccttcttc tttaaataaa tgcaagtact gtggtaaaat ttatattcct
    16081 acaaattcta aatctcaata ctgttcacct tcttgtagaa attgctcaaa tgtaaagaaa
    16141 agtagaagta gagccaaaaa agataaataa aattaataaa tatatcattg tttagatttt
    16201 agtaaataac tactgaatta aaagttgtta tataaaatta tatatctaaa tgatggtatt
    16261 ttttttgttg agaaaattga aaagtttaat tgagatagta attttgagtt gacaaaaacc
    16321 acttttaaca ctactattat aagtggttgg taaaataata tatactaatt caaatatagc
    16381 ataaaaaaca aaaaaagtca atggaaaaaa taaaaaagga gaatttaaaa atgatatcac
    16441 ctattatttt gagttctata aatcagaatt taaaggaaat agaaagaaat gaactgcttg
    16501 aaactaatat agaatctgga gattatggtc tagctctttc tgaatctgat gtaaaagata
    16561 taataaattc gagagataat actttaaagg ggtatggaag aatagaatta gatataaaag
    16621 ttacaaaaca gttaatagaa aatatatata catcacaata tacaaatgtg gacaattatt
    16681 tagaggctat taatgatatg caagaaattt tttattattt aaaaaatgaa actgatgaca
    16741 agatatgtga tgatgagatt atagaaatac tgggtgattt ttatgaaaaa ttttcaggca
    16801 atatggacaa tgtaagagga gaagcagacg aatttgcaaa gaaatttaaa tttggagaag
    16861 tttaatatat gataggagag attttgtatg ttaaataatt taagtaatat taatataaac
    16921 gaaaataatt taataaagaa ccaatattcc atatctctac ttaaggaatg tctaaattgt
    16981 aaagtgatag atgaaagaga agtatataat atacaacaag aaatatctct aatacttatg
    17041 gatttgatta aaaaatatac aaatggtcaa agcacatcag taaaaacaga agttgccgaa
    17101 aaactgttga tttcaatatg gtatgctatt gatgcatata taaataagct tggagatata
    17161 gaaaaaagag ttgtggagtt gactgctaag aatgttaaaa aagtgtacct tggaggaatt
    17221 gaaatattag aggaagagtt agttaggcta aaagattttt ataaactaat gatgagtaga
    17281 aaattggata ctaattcaat agcatataat gataccttga tggagttatc aagtttcttt
    17341 aaaagttata ttgtaaaatt tgaagctcag gacgttccag taagtataga ttatccattg
    17401 gcactagatg atatggatgt taagggtatt tattatgtga agaattatat tgagagtata
    17461 gatatagaaa atagattttg taacctattt aagaaaaaag atagagaaaa attattttat
    17521 gactatgggg tgacttataa aatagattgt gatgttatac ttgtaaattt atttgaactt
    17581 attatgaata attgtatttt ttctactatt ttaggaaatg aagtgactaa tttagaaatt
    17641 tcaaaatatg agtatgaatt tttagaaaaa caatttaaaa atatgtacaa taatattact
    17701 gaagataaag ttatagactc tgaagaaagt aatataattg aattgattga agtagatgat
    17761 agttttaaca ctgaagtaga tagtaatttg gattataatg attttgaaga aattaacaga
    17821 gaaattagaa taaataaaaa aataacttta ctacttatgg aagcaattga agtattaata
    17881 aaaagattaa gcatagaaga tgaagatgta ataagatata ttaaaaaata tgaaaagata
    17941 tttattgaat ctgtgctaag ctcaataaaa agtaacactt ttaaaagtat ggtaactgtg
    18001 agtaaaaata aaaatgaaca cttagaagat tatattgttg atgatgaaag taaattggat
    18061 gatgaatcgt ttagagagat attcaatgag atattagata gtacatctat aattgaaaaa
    18121 ataagaataa taaaggaaaa tatcaatgct aaaaaagact ttatcgatat tcttgaatct
    18181 gaatgtttat ttggagaaga gtatttaatg ttgtttgcat cattaagtga attagaattg
    18241 gcaatattgg gtagtgtagt attttatgaa gatattagaa tgagagaaat taatatatta
    18301 gaatttatgt taaatgagaa aactgaaact actttatgga agatagagta cattaaattt
    18361 atgaagaggc aaagtgaaga taagatatac tccatagaag agcatatgaa taaaattaat
    18421 taacaaaaaa ttgtgaataa atagaatgtt agtatataat ataatctgta aagcatatca
    18481 aaaatacata tatttggagg aaaattatga aagagtatat cataaataat gttgaagaaa
    18541 taagagaaga attaattgat ttatctaaga aaatatggga aaatccagaa ttagcctttg
    18601 aagaaaagta tgcaagtagc attcaaaaag aatacctaaa gtcaaaagga tttaaaattg
    18661 aagaagtaga aaatttacct actggattta tagcaagttt tggagagggt aaacctgtga
    18721 ttggcatact tggagaatat gatgctttac cagaactatc acaatgtgta agtgcagaaa
    18781 gaaagccatt ggtagaagga aaagctggac atggatgtgg gcataacctg ttgggtgtag
    18841 caggtgtagg agcagttgtt tctataaaaa aattaataga agaaaataaa tttaatggaa
    18901 caataaaata ttttggttgt cctgctgaag aagaaggtgg aggaaagaca gttatgtgta
    18961 taaatggctg ttttgatgat gtagattgtg catttacatg gcatcctttt gatataaatg
    19021 caccttggag aggaggaagt ctggcaaact tatctgtaaa atttaaattt aaagggataa
    19081 cagcccatgc agctcaagct cctcataatg gtagaagcgc acttgatgct gttgaaatta
    19141 tgaatgttgg agctaattat ttgagagaac atgttattga ctctataaga atgcactatg
    19201 ttataactaa tggtggtgga agacctaatg tagttcctgg atttgctgaa agttggtact
    19261 ttattagagg taaaaaagca aaagatgcag aacacgttct tgatagatta ataaaagtag
    19321 ctcaaggagc agcaatgatg acagaaactg aaatggagta taaggttaca gatggaatat
    19381 atgactatat accaaaccaa tgtttgactg atttagttta tgacaatatg gtttttgttg
    19441 gttgccctaa aaatactcct gaagaagaag aatttgcaaa aaagttgtgt gatactctta
    19501 ctagagaaga aagattaggt gtagcaacat cattacaaaa tgataaatct attgtagaaa
    19561 gctatataca caaggaaata gttgacggtg acaaagataa aggactagct ggttctactg
    19621 atgttggaga tgtaagttat gtaataccag tggctcaatt tgcaatggca gcttggcctg
    19681 ttggaattgc aagtcataca tggcagtcat gttcatcagc aggttcaaat ataggatttt
    19741 ctgctatgat aaattcagct aaagtactgg cttgtagtgc ttatgatgta tttatggata
    19801 ctaaaattat tgatgaagca aaaattgaat ttgataaatc attagatgga caaaaattta
    19861 aaccattggt ataattttgg taataaaata tttcaggggg gaatagctat gagagaaaaa
    19921 gttgaaaaag tgcttgagga aaagataaaa ccagtattac aaagagatgg tggagatgta
    19981 gagcttatag atgtaaatga aaatggtgtg gttttagtta gactacaagg ggcatgtagt
    20041 gggtgtccag gagcgactat gacaatcaaa gctattattg aaaatgtttt ggtgagtgaa
    20101 gttccaggag taactcaagt attaggtgtt taatagttag cgtttattaa atgtatgtta
    20161 tacataagta tttaataaag aagtaaaaaa aatagtgaaa caatatatat gaa
//
LOCUS       DAGSRM010000069        19755 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.069, whole genome shotgun sequence.
ACCESSION   DAGSRM010000069 DAGSRM010000000
VERSION     DAGSRM010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19755
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.069"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            41..1108
                     /locus_tag="MJE33_002612"
     CDS             41..1108
                     /locus_tag="MJE33_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HBY9648541.1"
                     /translation="MKNKLVYICIMSLLLFVVGCSNNPNKESTIKKDSKNNQSQVKEE
                     KREPVKKDITISFAGDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLKNVKPVFEKDDL
                     TVVNLEGPLTTASNAKEKKFAFKGKPGYVNILKSGSVEAVSIANNHSEDYFEEGMKDT
                     QFILDENKINYFGLGKDAVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIKSLKKKS
                     DVVVVYFHWGIELDYYPDKKQKDFAHYAIDKGADLVMGSHPHVIQGIEKYKNKYIAYS
                     LGNFCFGGNKNPSDTDSYIYQQTFTFSDNKLTSIKEPNIIPTSITSSNSRNNYQPKIL
                     DGSEKDRVLNKLEKISKDLNK"
     gene            1278..3179
                     /gene="cooS"
                     /locus_tag="MJE33_002613"
     CDS             1278..3179
                     /gene="cooS"
                     /locus_tag="MJE33_002613"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBY9648542.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVVKGEKTLNQLAEMFGIESDEKYDKCIKVADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFKLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAECIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            3172..3645
                     /locus_tag="MJE33_002614"
     CDS             3172..3645
                     /locus_tag="MJE33_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBY9648543.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSRHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRG"
     gene            3620..4846
                     /locus_tag="MJE33_002615"
     CDS             3620..4846
                     /locus_tag="MJE33_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBY9648544.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLH
                     HVISEHRTLKQINFVDEDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVT
                     GVAFSNGEVLDADMVIVATGVKPNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGINDYKKFVFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDICY
                     ADFFNIKENGEFCYSV"
     gene            complement(4985..6172)
                     /locus_tag="MJE33_002616"
     CDS             complement(4985..6172)
                     /locus_tag="MJE33_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBY9648545.1"
                     /translation="MNITKNFLKDYLEKFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNARIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRVVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKNFS
                     ENLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTREYMYN
                     CSK"
     gene            6445..7269
                     /locus_tag="MJE33_002617"
     CDS             6445..7269
                     /locus_tag="MJE33_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBY9648546.1"
                     /translation="MEVYALVGSSGTGKSFKALEFAYENDIEYIIDDGILIYKNKVLA
                     GISAKQANTTIEAVKRAIFYNLEHRQEVREAIKKENIRKILIIGTSKKMVNQIVERLS
                     IGRVCKFINIQDISTKYEIEIAKQARKEGNHVIPVPAVEIKSMASGLSINSLKRLFRK
                     GNNRNMVVLEKTIIRPTFSYIGKFYISADVIKQIIEYEVYNFGSIDRINKINIENCNN
                     FMNIFVSININDLETIKKIEDIQQTIKKSIEKMTLVNVQKIDINIHKIKKINFLQC"
     gene            7398..8663
                     /gene="gluD"
                     /locus_tag="MJE33_002618"
     CDS             7398..8663
                     /gene="gluD"
                     /locus_tag="MJE33_002618"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HBY9648547.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            8876..9466
                     /locus_tag="MJE33_002619"
     CDS             8876..9466
                     /locus_tag="MJE33_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HBY9648548.1"
                     /translation="MHFKSKDLNLKYLVLFLKIVYWFNPFICIMEKYMDLDCEFYCDE
                     RVLKNCTVQQKQEYALTIVNSMKRGTNFSNKFVAGLNKQSDIIKRVMNMFSEKHKNGI
                     LMALIISLFSSITFFKFDSTSIIEENDFLERLRYTFEGRQEDMFVTIDFTYADTPIKL
                     RKEHEENCRVNGIVPKDSDSIEIIPRFYKDCMDLKK"
     gene            9796..10929
                     /locus_tag="MJE33_002620"
     CDS             9796..10929
                     /locus_tag="MJE33_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HBY9648549.1"
                     /translation="MSNFILNGIFRTVIVSSLSILLILIFKKNVFKRFSKKFNYYIWM
                     IVVIKLFLPFTYYTFTINILRSKKHININNINLEGFNNVSTISNNIILYTWIITVVVY
                     LIYTIFKYIKLKNLINDLSYDVDDREIVNLYKNILEEFNITKDIKLKYSYEVETPAFF
                     NSCVLLPPHEYKLKELDWIFRHELMHFKSRDLYLKYVILFLKTVYWFNPFIYIMDKHI
                     DLDCELYCDERVLKNRNSEEKKDYALTILNAMRKGSNTSNKFIAGLHKQSDIKKRVMN
                     MFNEKYKNGILMALTLCLLSSITFLKVNSTSIVNLNVLPKGFEDLRTVSSIRFSVIGF
                     TYADAPKNMREEYKINCESLGLIPKDSDKIEVGIEYYDSLLND"
     gene            11845..12867
                     /locus_tag="MJE33_002621"
     CDS             11845..12867
                     /locus_tag="MJE33_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBY9648550.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEDNGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQVNPSIDQNVDSTNNNQQNNANTNQQNNNSNVPTANSNA
                     VQAVLNLAYSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSKAQSKYGQTV
                     SKANLQPGDLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVSVTSINSSYYTARFV
                     TAKRIL"
     gene            13163..14083
                     /gene="pyrB"
                     /locus_tag="MJE33_002622"
     CDS             13163..14083
                     /gene="pyrB"
                     /locus_tag="MJE33_002622"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HBY9648551.1"
                     /translation="MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDS
                     DDRAVYFKQAKYGMYVRMALIIKLLGINED"
     gene            14122..14814
                     /locus_tag="MJE33_002623"
     CDS             14122..14814
                     /locus_tag="MJE33_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBY9648552.1"
                     /translation="MYKILENIYIGEDMYRMKVKGNFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDNIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILEDEYKQVCNKVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTPMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNGGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            14807..15709
                     /locus_tag="MJE33_002624"
     CDS             14807..15709
                     /locus_tag="MJE33_002624"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBY9648553.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETPSGMMNSVGLENPGVQGFIDYELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVREVRNITKLP
                     LVIKLSPNAEDIVGMAKVCEEEGADGVSLVNTFKAMAIDIKNRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIRGII"
     gene            15794..16378
                     /locus_tag="MJE33_002625"
     CDS             15794..16378
                     /locus_tag="MJE33_002625"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBY9648554.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            16593..17519
                     /locus_tag="MJE33_002626"
     CDS             16593..17519
                     /locus_tag="MJE33_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HBY9648555.1"
                     /translation="MKNQLNENHKKVVFFGVGAVGATFAEQFFNSKYDFKILCDNERK
                     KRYLEEGFIINGKRYDFDYVTKDEYKQEADFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIEKMVYSYVTNIDAKKINNNIIHTTNGIIVFGNKDNSEDR
                     KTNIITEVFDDVNIEYTLSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKNISLTEDDVEHSLHRILEHSKEGRTSMLQDVEAHRLTEVDMF
                     SKNICKLGKKYNIPTPINQTFFYMIKVIESRF"
     gene            complement(17571..18461)
                     /locus_tag="MJE33_002627"
     CDS             complement(17571..18461)
                     /locus_tag="MJE33_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBY9648556.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSKKYPDIDVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYKSHLLKDFSFDKLQNQKWIVREDGSGTRDYLDMFLSVKEIIPKSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
     gene            18649..19680
                     /locus_tag="MJE33_002628"
     CDS             18649..19680
                     /locus_tag="MJE33_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBY9648557.1"
                     /translation="MLKKKSSLTLKTIREILPGLFVSVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGATVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKIVRVIFLVVVVLVFGHMKNQSNREILEEEKDEIKKKKVTIPWYVIGFFITCALF
                     SMNIIPQEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFVGTVQVVS
                     AVVLIKIFI"
BASE COUNT         7754 a         2087 c         3497 g         6417 t
ORIGIN      
        1 tattttattt aaaaatttat taatacaaag gagatttact atgaaaaata aattagttta
       61 catatgtatt atgagtctat tgctatttgt agtaggatgt tctaataatc caaataagga
      121 aagtaccatt aaaaaagact ctaagaataa ccaaagccaa gttaaagaag aaaaaagaga
      181 acctgtgaaa aaagatataa ctataagttt cgctggtgat gtaactatgg ggaactataa
      241 aggttcatca tattatggca gttttgacca tgaatttaaa agacaaggag aagattacaa
      301 ttattttctt aaaaatgtaa aacctgtatt tgaaaaagat gacttaacag tggttaattt
      361 agaaggtcca cttactacag cgtcaaatgc aaaagaaaaa aaatttgcat ttaaaggaaa
      421 gcctggatat gtaaatattt taaaatctgg aagtgtagag gcagtaagta ttgcaaataa
      481 tcattcagaa gattattttg aagaaggaat gaaagataca cagtttatat tggacgagaa
      541 taaaataaat tattttggat tagggaaaga tgctgttgta gatgtcaaag gaattaaagt
      601 gggtctatta gggtataatg gattatcaac tgaatataat gaaaaaaatt taaaacaaat
      661 ggaagatgat attaagtcct taaaaaagaa atcggatgtt gttgtagtgt actttcattg
      721 ggggatagaa ttagattatt atccagataa aaaacaaaaa gattttgctc attatgctat
      781 agacaaggga gcagatttag ttatggggtc tcacccacat gtgattcaag gaatagaaaa
      841 gtataaaaat aagtatatag cttatagtct agggaatttt tgctttggag gaaataagaa
      901 tccttctgat acagatagtt atatatatca acaaaccttt actttttcag ataataaatt
      961 aacttcaatt aaagagccaa atataatacc aacttcaata acatctagta actccagaaa
     1021 taattatcaa ccaaaaatat tggatggaag tgagaaggat agagttttaa ataaacttga
     1081 aaaaataagc aaagatttaa ataaataaaa tgtaaaaaaa aataaatata tatatttatt
     1141 ttaataattt atattttgtt tagggtatgg taaatcgtgg tattaaataa gtagatttta
     1201 atagttaaat tatagtcaat tataataaag ctatttaatc tactttttta atataaaaat
     1261 ataaggggga atttgtaatg gcttgtaaca cttgtaaaat gtgcgaaagt gcagacaaga
     1321 aattagaaag ttttgtagct tcaaaggatg ttgaaacagc atttcataga acagaagatc
     1381 agaaagttaa gtgtggtttt ggacttcagg gggtttgttg tagactctgt tcaaatggac
     1441 catgtagagt aactcctaaa tcaccaagag gtatatgtgg agcagatgca gacactatag
     1501 tagcaagaaa ctttttaaga gcagtagcat caggagcagc ttgttattta catgtggtag
     1561 aaaatacagc taagaattta aaaaatgttg gaataactaa aggtgttgtt aaaggagaaa
     1621 aaacattaaa tcaattagca gagatgtttg gaatagaaag tgatgaaaag tatgataaat
     1681 gtataaaggt tgcagacaaa gttatcaacg atttgtataa atcaagagat gataaaatgg
     1741 aattagtaga aaaaatagct tatgcaccta gagtgaaaaa gtggaaagag ttaggtataa
     1801 tgcctggtgg tgcaaagtca gaagtttttg atgcaatagt aaaatcatca acaaatttaa
     1861 acagtgaccc agtagacatg ctagttaatt gtttgaactt aggtatatca acaggattat
     1921 atggtcttac actaactaat cttttaaatg atgttatgtt gggagaacca gttataagaa
     1981 tggctcctgt aggatttaat gtaatagacc cagactacat aaatataatg ataacaggtc
     2041 atcaacattc aacatttgca aatttccaag atagattaaa agatgaagat gttataaaat
     2101 tagctcaatc tgttggcgca aaaggattta agttagtagg atgtacatgt gttggtcaag
     2161 atttacaatt aagaggagaa cattatcaag aagtatttgc tggacatgca ggtaacaatt
     2221 ttacaagtga ggctgtttta tcaactggag caatagatat agttctttca gaatttaact
     2281 gtactatacc agggcttgaa ccaatagcag ataaatataa agtaaaaatg gtttgtttag
     2341 atgatgttgc taaaaaagcg aatgcagaat gcataggttt agacagaagt aaattagatg
     2401 aacttagtaa cactttaata gaaaaagcat tggagtctta taaggagaga agaggaagta
     2461 ttgaaataga tataccaaaa gaccatggat ttgaacaatc tttgactggt gtaagtgaaa
     2521 agaacttgaa agcgttttta ggagattcat ggaaacctct tattaattta attgcagaag
     2581 gaaaaattaa aggtgttgca gctgttgtag gatgttcaaa tatgacagca ggaggacatg
     2641 atgtaaatac tgttgaactt actaaagaat tgattaaaaa agatataata gtactatctg
     2701 caggatgttc tactggagga ttagaaaatg ttggtcttat gtcacctgga gcagaagaat
     2761 tggcaggaga aaacttaaaa gaagtatgta agactttagg aataccacca gtattaaact
     2821 ttggaccatg tctagctata ggaagactag aaatagtagc aacagaatta gcagctgaac
     2881 ttggaattga cctaccacaa ttaccattag tgctttcagc tccacaatgg ttggaagaac
     2941 aagcattagc tgatggagct tttggtcttg cccttggctt acctcttcat ttagctttac
     3001 ctccatttat aacaggagga aaattggtaa cagaagtatt aacagaaaaa ttaaaagact
     3061 taacaggagg acatgttata gtgaatccag accctaaatc ttcagctaat caattagaag
     3121 aaataataat agatagaaga aaaaatttag ggttaaagga tgtggaatgc aatgcataga
     3181 atttttataa ataaagattt atgtacagga tgtaagagct gtgtactagc atgtatgcta
     3241 aaacacaata aagattatga catgtacact cttgatttag aaaatataga taatgatagt
     3301 agagggcata tagaattaga tagtagacat aataatccag ttccaattct ttgtagacac
     3361 tgtgatgaac ctgaatgtgt attagcatgt atgagtggtg caatgcataa agatagtgaa
     3421 agtggaatcg tttcatatga tgaagagaaa tgtggttcat gttatatgtg tgtaatgtct
     3481 tgtccatatg gattattaaa gccagatgat agaagcaagc agaacatact taaatgtgat
     3541 ttatgtaaag atgaggagta tcctagatgt gtagctaatt gcccaagtgg agcaattgaa
     3601 ttacagaagg aggaaaatga tgaattatgt agtgttaggg gctagtgcag ctggtataaa
     3661 tgcagtaaaa acattaagag aattagataa agattcaaat atagtagtaa tttctaaaga
     3721 tgaaaacgta tattcaagat gtatgttgca tcacgttata tctgaacaca gaactttaaa
     3781 acaaataaat tttgttgatg aagattttat ggagcaaaat aatgtaaaat ggattgcagg
     3841 taagactgtt aaaggtatag acataaataa aaaggtagtt cagacagaag acattactgt
     3901 aaattatgat aaacttttaa tagctacagg tgcatcttct gctattcctc ctataaaaaa
     3961 tttaagagat ggaaattttg tatattctgt gagaaacata gatgatattt ataaaataaa
     4021 agaaaaagca caaaattcta aaaatgtagt tataataggt gctggtttag ttggtataga
     4081 tgcattagtg ggattattta aatatgagca gttaaatatt tctgtagctt ttatggaaaa
     4141 gtacatttta gatagacaat tggatgaata tacagcatca gtgtatgaaa ataagttcaa
     4201 agaaaaaggt gtaaaatttt acccaagtgc atcaatacaa gaaattgtat tggataacag
     4261 taaaaatgtt acaggtgttg cattttcaaa tggtgaagtt ttagatgcag acatggtaat
     4321 tgtagctaca ggggttaaac caaatgctga ctttttagat ggaacaggaa tagaatatga
     4381 tagaggtata ataatagatg atatgtgcca aacaacacaa aaagacatat atgcagcagg
     4441 agatgttgta ggtaaaaatg ctatttggcc tctggcagta aagcaaggaa ttgtagctgc
     4501 ctataatatg gttggaaaag ataagaagat agaagatgaa tttgctttta aaaatagtat
     4561 gaatttcatg gatataccaa ctatttctat aggaatgaat acacctgtag atgatagcta
     4621 taaagtatta accagacatg gaattaatga ttataaaaag tttgttttta aagataatgt
     4681 aatctatgga gcagttatac aaggagatat ttcatatgta ggtgtattaa cttatttaat
     4741 aaaaaataaa gttgaaatat atgatttaga aaataggata tttgatatat gttatgcaga
     4801 cttttttaat ataaaagaaa atggagaatt ttgttatagt gtatagtata tacgtggagt
     4861 aaggtttata aatacagtaa atcaataaat agagttattc taaaataaga aaaatgacaa
     4921 atcttattta gaaatagctc tattttttgt aattaaattt aaattatatt caatttgtaa
     4981 aatattattt tgaacaatta tacatatact ctctagttag aggtaaatca ttttttatac
     5041 ctttagtgaa aagtatttga tgtatatcta taataccagt gttgaaagca gcagcacaag
     5101 aacaaaggta taaatcccac attcttataa acttatcatc aaacatttct ttaatctttg
     5161 gaagattctc agagaaattt ttttgccagt atagtaaggt ttttacataa tgtaatctta
     5221 aactttctac gtctatagta tgatatttgt aatcactaca aatatgtatc atttctctta
     5281 ggctaggaat aactcctcct gggaaaatat atttttttat ccaagggtct ccataagatt
     5341 cctgcaatcc acttatatag tgaagtaaaa acaatccacc atcttttaaa acagcattta
     5401 cgtttttaat gaaaagttca tagttttctc taccaacatg ttcaagcatc ccaacgctta
     5461 caactctatc aaacataagg tctgattttt ttaattctct atagtccata agtcttacct
     5521 caagatagtc ttgaaggttc tcttctttaa ttcttgcgtt aaattcctta aattgctctt
     5581 cgctaagtgt aattccaacg ccgtgaattt tatatttttt tgcagcttct attagtaaaa
     5641 atccccaacc acaaccaata tcgagaaggc tcataccttc ctctaaattt agtttagtta
     5701 atatgtggtg aactttattg acttgagcat cataaagagt atcattatca tttctaaagt
     5761 atgcacaaga ataactaagt gtcttatcta accataagct ataaaaatcg ttgccaatgt
     5821 catagtgggc atgtatttct ttcttttgat gtctcattga atttgatgag tgcaatagat
     5881 gttttacagc tgaattatca atattgaatt tgtctatttg ttttaaaagt agatttaaag
     5941 ttaagaataa atctccctca acttcaatgt ctctatttat gtaggcttct ccaagtgcta
     6001 aagatgtact ttttaatagt tcagacttgc taatatcatt atttactata actttgaact
     6061 gaggggagcc ctctccaatt agaaattctt cctcatcttt aagcctaact agaaatggtg
     6121 tttcaccaaa tttctctaaa tagtctttta aaaaattttt tgtaatattc atattaccag
     6181 ctccttctaa tgtaaatcca gtaagtttcc ctgcttaaaa tatattatag tccttttttt
     6241 aaaaaagtaa aattttttca ataaattttt tatttaaagg aaatttattt tttgtaaaga
     6301 aatttcatgt tgtagtatat tttttgctta taaattttgg tactggatat aatattaaac
     6361 tgttaatgat ataataaatt taatctataa agtaaataaa attttgtaat aaatagttaa
     6421 agctatttta taaggagata tatgatggaa gtatatgctt tggttgggtc aagtggcaca
     6481 gggaagagct ttaaggcttt agagttcgca tatgaaaatg atatagaata tataattgat
     6541 gatgggattt taatatacaa aaacaaggtt ttagctggga tatcggcaaa acaagcaaat
     6601 actactatag aagcagttaa aagggctatt ttttataatc tggagcatag acaagaagtt
     6661 agagaagcaa ttaaaaaaga aaatataaga aaaattctta ttattgggac atcaaaaaaa
     6721 atggttaatc aaattgtaga gagattgtca attggaaggg tttgcaagtt tataaatata
     6781 caagatataa gtactaaata tgaaatagaa atagcaaaac aagctagaaa agaaggaaat
     6841 catgtaattc ctgttccagc agttgagata aaaagtatgg caagtggtct tagtataaat
     6901 tctttgaaga gactttttag aaaaggaaat aataggaata tggtagtttt agaaaaaaca
     6961 attataagac caacttttag ttatatagga aagttttata ttagtgctga tgtaattaaa
     7021 cagattattg aatatgaagt atacaacttt ggaagcattg atagaataaa taaaatcaat
     7081 atagagaatt gcaataattt tatgaatatt tttgtaagta taaatatcaa tgatttagaa
     7141 actataaaaa aaattgaaga catacaacag acaataaaaa agtctattga gaaaatgact
     7201 ttagttaatg tacaaaaaat agatattaac attcataaaa ttaaaaaaat aaacttcctc
     7261 caatgttaaa aaataaactg agaaaatgat atactaattt gcaataaagt gtgctataat
     7321 taaactgtaa atttaggaaa tggttttttc taaatggagt atacataatt ataacgtatt
     7381 tataaggggg ctttcaaatg tcaggaaaag atgtaaatgt cttcgagatg gcgcaatctc
     7441 aagtaaaaaa tgcatgtgat aaattaggta tggaaccagc agtttatgaa ttattaaaag
     7501 aacctatgag agttatagag gtttcaattc cagtaaaaat ggatgatggt tctataaaaa
     7561 cttttaaagg atttagatca caacataatg atgcagtagg gccaacaaaa ggtggaataa
     7621 gattccatca aaatgtttca agagacgaag taaaagcttt atctatatgg atgactttca
     7681 agtgttctgt aacaggtata ccatatggtg gaggtaaagg tggaataata gtagatccat
     7741 caactttatc tcaaggtgaa ttagaaagat taagtagagg atacatagat ggaatatata
     7801 aattaatagg tgagaaagtt gacgttcctg caccagacgt aaatactaat ggtcaaataa
     7861 tgtcttggat ggttgatgag tacaataaat taactggaca aagttctata ggtgttataa
     7921 ctggtaagcc agttgaattt ggtggttctt taggaagaac agctgctact ggatttggtg
     7981 ttgctgttac tgcaagagaa gctgctgcta aattaggaat agatatgaaa aaagctaaaa
     8041 tagctgttca aggtatagga aacgttggtt cttatacagt tcttaactgt gaaaaacttg
     8101 gtggtactgt tgtagctatg gctgaatggt gtaaatcaga aggttcttat gctatataca
     8161 atgaaaatgg tttagatggt caagctatgt tagattatat gaaagaacat ggtaacttat
     8221 taaacttccc aggagctaag agaatatctt tagaagagtt ctgggcttca gatgttgata
     8281 tagtaatacc agctgcatta gaaaactcta taactaaaga agttgctgaa tctataaaag
     8341 ctaaattagt ttgtgaggct gctaatggac caactactcc agaggctgat gaagtatttg
     8401 ctgaaagagg aatagttctt actccagata tattaactaa tgctggtgga gttacagttt
     8461 cttacttcga gtgggtacaa aacttatatg gatactactg gtcagaagaa gaagtagagc
     8521 aaaaagaaga aatagctatg gttaaagcat ttgaatctat ttggaaaatc aaagaagagt
     8581 acaatgttac aatgagagaa gctgcttaca tgcattcaat taagaaagtt gctgaagcta
     8641 tgaaattaag aggatggtac taagatttac tcttaataaa taataggata taattattta
     8701 agatataaag gggctgtaca tagtacagcc cttaaattat gtggcttaca atgaagtaag
     8761 ctatttttgt tttaacttat aaataacttg ataaattatg taatttagaa tgtatttttt
     8821 accaccacat gattacaaat tagaggagtt agattgaatt tttagacatg aacttatgca
     8881 ttttaaaagc aaagacctta atttaaagta tctagtatta tttttaaaaa tagtatattg
     8941 gtttaatcca tttatatgta taatggaaaa atatatggat ttggattgtg aattttattg
     9001 tgatgagaga gttttaaaaa attgtactgt acaacaaaag caagaatatg ctctaactat
     9061 tgtaaattca atgaaaagag gtacaaattt ttcaaataaa tttgtagctg gattaaataa
     9121 acaatctgat attataaaaa gagtaatgaa catgttcagt gaaaaacata aaaatggtat
     9181 cttaatggct ttgattataa gcttgttttc ttctattaca ttttttaaat tcgattcaac
     9241 tagtattatt gaagagaatg atttcttaga gaggttaaga tatacgtttg aaggaaggca
     9301 agaagatatg tttgtaacaa tagattttac atatgcagat acacctataa aacttagaaa
     9361 agagcatgaa gaaaactgta gagtcaatgg aatagtacca aaagattcag attcaataga
     9421 aattattcct agattttata aagattgtat ggatttaaaa aaataagtta gacttaatat
     9481 tgatgttgat attatgaatt ttttatattt aagcaattga aaattttatt atagattcaa
     9541 ttttatatgt gtttaagtat gaaatttata gatagttgaa ataattaaat atgattgatg
     9601 cataggaatc attactaagc tataattaat tataggggag tttaaataaa ttatgagtta
     9661 cacaattata aaatgataaa aatagttatc tgttacaaaa aatgtatctt tttaattaat
     9721 aaagataata tgaaaactta aaaggcaaat aaacttaaat ataaaatact cttaaataag
     9781 ataggagaaa aatgtatgtc taactttatt ctaaatggga tttttagaac agttatagta
     9841 agtagtctaa gcattcttct gatattgatt ttcaagaaaa atgtatttaa aagatttagc
     9901 aaaaaattta attattatat atggatgata gttgttatta aattattttt accatttaca
     9961 tattatactt ttacaataaa tatcttgaga agtaaaaaac acattaatat taataacata
    10021 aatttagagg gatttaataa tgtttcaact ataagtaata atattatatt gtacacttgg
    10081 attataactg tagtagtgta tttaatttac actatattta aatatataaa acttaaaaat
    10141 ttaataaatg acttatctta tgatgttgat gatagagaga ttgtaaatct ttataaaaat
    10201 atattagaag aatttaatat aacaaaagat ataaaactca aatactctta tgaagttgaa
    10261 acaccagcat tttttaattc atgtgtactt ttgccaccac atgagtataa attgaaagag
    10321 ttagactgga tttttagaca tgagcttatg cattttaaga gtagggacct ttatttaaaa
    10381 tatgtaatcc tgtttttaaa aacagtatat tggtttaatc catttatata tataatggat
    10441 aaacatatag atttagattg tgaactttat tgtgatgaaa gagttttaaa aaatcgaaat
    10501 tcagaggaaa aaaaggatta tgctttaaca attctaaatg caatgagaaa aggttcaaat
    10561 acttcaaata aatttatagc tggactccat aagcaatctg atattaaaaa gagagttatg
    10621 aatatgttta atgaaaagta taagaatggt atattaatgg cattaacttt atgtttatta
    10681 tcttctatta cttttttgaa ggttaattca actagtattg ttaatcttaa tgtattacct
    10741 aaaggatttg aagacttacg aactgttagc agtatacgtt tttcagttat aggatttaca
    10801 tatgcagatg ctcctaaaaa tatgagggaa gaatataaaa taaattgtga atcattaggt
    10861 ttgataccta aagactctga taaaatagaa gttggtatag aatattatga cagtctattg
    10921 aatgattaaa taaatattat tttaacagat attaattata aagttttgaa tagtaatttg
    10981 ttgagaaata aatatatcct tataaaaata gatacttata taccaagaat aaaaaatagt
    11041 caagatagta cacaaggata tacttataaa gaacttaaat aagtgaaata aattattaat
    11101 tataaggttt ataattttaa aaaattagat aaattcaggt gtaaaatact ataaaacaca
    11161 attcggaaaa gtgtatgttg aacttcattt aaaatggaat ttcaaagttg ctatagtaag
    11221 tagtttaagc attcttctta tattaatctt tgagaataat gtatttaaaa aagttagtaa
    11281 aaaatttaac tattgtatat taatgatagt tgctattgat atattatcta aaaaaattaa
    11341 gtatgaaaag attactgcta ggacaatctt ttcatactta atttttaata tttatattat
    11401 ccaaaatttt catcttttta acttagaatt tgagaatcac tattaggaaa taggctttga
    11461 tttatgtagg gttaaaaatt tcacagcata tctaacttta tactaaatcc atcaaaaaat
    11521 atacgcccta taaaagcgca taaaaaaaca cataaaacaa tatttatgcg cactttaacc
    11581 tcgaatggta caaaattctt tacttttaca gacccatatt atataattaa gtaaataatg
    11641 tatatttagg tgacatattg gataagctaa tttatttatt ttattaatat aatgatacta
    11701 taaaagttat ataaagtggt ttacaaaaaa gttacagata gagcttttat taatgtattg
    11761 tgtattagta ttttatcctt aggtacactt cacctacaaa atagaatcat aagataatta
    11821 tgggaaaatt ttaggaggtt tatcgtgaag aaaaagatat taataccagt atttgcatca
    11881 gttatggcat tatcagtaag ttcaattgta aatgcagatg aagtaaatga ttcatctcaa
    11941 aataaggatg ataaaacaaa tactgaatta aatttaggtg aatataaaga agtaaaatat
    12001 aaagttgcaa aaataaaaga tggagtagct ataaagataa gagaagaagg tcaagttcaa
    12061 aacatagctt attctggaga tgaatttacg gttttaggaa ctcaaggaga atgggttaag
    12121 gttaaagttg aagatggcga aggttggtta gctactagat atgtagatat atctgaaggt
    12181 gtaggatata ctaacgctga taaagtaaac ttaagaaaag ataagagtga aagttcagaa
    12241 gtaattgaag agctggaaaa aggaagttct ttattggttt tggaagataa tggagattgg
    12301 ttaaaagtta aagatgggga aacagaagga tatgtaaaat catcttatat atctgataaa
    12361 gctcctgtaa tagaagagcc acaagtaaac ccaagtattg accaaaatgt agattctaca
    12421 aataataatc aacaaaacaa tgcaaatact aatcaacaaa ataataattc aaatgtgcca
    12481 acagctaact ctaatgcagt tcaagctgta cttaacttgg catattctaa gcaaggttgt
    12541 ccatatgttt ggggagcaga aggaccaaac acttttgact gttctggatt tacacaatat
    12601 gtgtatagaa atgcagtagg aaagaatata ccaagaacat ctaaagcaca atctaaatat
    12661 ggacaaactg taagtaaagc aaatttacaa ccaggagact tagtattctt cacaactaat
    12721 ggttctggtt ctgttagtca tgttggaata tatgtaggtg ggggtaatat gatacattca
    12781 ccatcaacag gaaaaacagt tagtgtaact agtataaatt catcatacta tacagcaaga
    12841 tttgttacag caaaaagaat attataatcg gtttgtaata tgacaaattg ataaatacat
    12901 aaatatatat aaacgctcta tattatattt aatttaatca tggaaacaaa aaatgattat
    12961 attttaaatg tactatagag cgtttttgta tcactttaaa tctaatcaag attcaagtta
    13021 atatgactgt taaataggag ccttttcata tgatgattat ttgtgcaatc ccttgacagg
    13081 atagggcaaa ggtggtaagc tactaacagt acaaaaatac ttttaatttt agttcagaga
    13141 ggctaaaaaa ggaggacgaa agatgttaaa atcaagaaat ttaatccaac cagaagactt
    13201 ttcaatagaa gaaatagatg aaatattaga attagctcaa aaaataattg acaatccgtc
    13261 aaaatattct cgtatatgtg aaggaaaact attagctaca ttattttatg aaccatcaac
    13321 aagaacaaga ttaagctttg aatctgctat gaacagatta ggaggacgtg tagtaggctt
    13381 ttcagagcca aattcatcct ctgcatcaaa aggtgaaact ctaggagata caatgaggat
    13441 agtatctggt tatgttgata taatagctat gagacatcct caatcaggtg cagcttcaga
    13501 agcagctaga tatacggaag tgccatttat aaatgcagga gacggcaaga accagcatcc
    13561 aactcaaaca cttacagact tacttactat aaaatcatta aaaggaactt tagaaagcca
    13621 tacaatagga ttatgtggtg atttaaaata tggaagaact gtgcattctt tagttaaggc
    13681 tatggcaaga tataaaaata caaagtttgt atttatagcg cctgaagagt taaaaatgcc
    13741 agattatata aaagaagcta taaagggaca tgcatattat gaaactaata atttggatga
    13801 tgttatagga agcttggatg ttttatatat gactagagtt caacaagaaa gatttgaaga
    13861 taaatcagag tatgaaagac ttaaaaacta ttatatttta aataaagcta aattagaaaa
    13921 ggcatctaaa gatatgttag taatgcatcc attaccaaga gtaaatgaaa tagacataga
    13981 tgtagatagt gatgatagag ctgtatattt taaacaagct aaatatggaa tgtatgttag
    14041 aatggcttta ataataaaac ttttaggtat aaatgaagac taatagttat catataatag
    14101 taaaacaaag gagattttgg gatgtataaa attctggaaa atatatatat aggcgaagat
    14161 atgtatagaa tgaaagtaaa aggtaatttt gaaggtaaaa tgggacaatt ctatatgtta
    14221 agagcatggg atacttatcc agtactttca agacctataa gtatacatga tatagatgaa
    14281 gaagggataa cttttcttta taaagtagta ggagaaggaa ctcaaatttt gagtaaccta
    14341 aaggtaaatg ataatataaa attggaaggc ccttatggta atggatatgc taaggttgat
    14401 ggaaaggtag ctctagtagg aggaggaatc ggtgtagctc cactatattt ggttgctaaa
    14461 aatataaaaa attgtgatgc atatcttggg tttagagaag atgttatatt agaagatgaa
    14521 tataaacaag tatgtaataa agtctataca acagtaggaa atacttttgt aacagatata
    14581 attgatgttg agaaatatga ttatatatta acatgtggtc caactccaat gatggaaaaa
    14641 ttagttaaga tggtagaagg tactaaaacg agaattatgg tatctcttga aaaccatatg
    14701 gcttgtggag taggtgcctg tttagtatgt acgtgtaaaa ctaatggagg aaataaaaag
    14761 acttgtaaag atggtccagt gttctgggga gaggacgtga tttttaatgg gtaatttaag
    14821 tgtgaaattt ggaagtgtag aatttaaaaa tccagtaata atggcgtctg gaacttttgg
    14881 atttggaaaa gaatataatg agatttatga tatacaaaaa cttggtggta taagtagcaa
    14941 gggtcttact ttaaataaaa aacctggtaa caatggtatg agagttcatg agacaccatc
    15001 cggtatgatg aatagtgttg ggcttgaaaa ccctggagtt caaggattta tagactatga
    15061 attacctttt tttagtaaac tagacttagt cagaatagct aatgttggag gtgggacatt
    15121 agaagactac ttattagggg ttcaaatgtt aaatgacaag cctatagaca ttattgaatt
    15181 aaatatatct tgcccaaatg tcaaagctgg tggaatggct tttggtataa aaaatgaagt
    15241 ggcaagagaa gttgttcgtg aggtaagaaa cataacgaaa ctgcctttag taataaaatt
    15301 atcacccaat gcagaagata ttgtaggaat ggcaaaagtt tgtgaagaag aaggtgctga
    15361 tggagtttca ttagttaata cttttaaagc aatggcaatt gatataaaaa atagaagacc
    15421 agtatttgaa aatgtatatg ctgggttatc tggaccagct ataaaaccta tagctttgag
    15481 aatggtacat gaagtgtgta aaaatgtaaa tatacctgta atgggaatgg gcggaataac
    15541 taaagccaca gatgctatag agtttataat ggcaggagct acttgtatcc aagttggaac
    15601 tgcaaatttt atgaatccta gaataggtat tgaaataata gatggaatta atgaatttat
    15661 ggatagagaa ggaataaaaa gtttagacga aataagagga attatataag gtagaataaa
    15721 tagtatttta aagataaaaa atatagatta aataaacaat aattttataa cattaacatg
    15781 gaggaaaaat aatatgagta aagtagatgt agtggatata ttaaagaaaa gtgatgcatt
    15841 gttggaggga cattttttat tatcttcagg aaaacacagt aatagatacg tacaatgtgc
    15901 aaaagtatta agatttccag aatatgctgc tgaggtatta agtacagttg ttgaacaaat
    15961 aaaagactta gatatagact tagtagtagg accagctatg ggtggagtaa tagtttctta
    16021 tgagttagga agacaattag gaaaagaagc tgtatttact gagagaaaag acaatacaat
    16081 ggagttaaga agaggatttg aagttaaaaa aggagcaaag ataataattg ctgaagacgt
    16141 tgtaactact ggtaaatcaa ctatggagac aaaaagagta ttagaagcct taggtggaga
    16201 agttgtaggt gttgcatgta tagcagatag aactaatcat gatataggta tgcctatata
    16261 tagtgctata aaacttgata ttcaagttta tgaatctgat gagtgtcctt tatgtaagga
    16321 aggaaaatta ccagttgtta agcctggaag tagggagttc aaggaattag ggatgtaata
    16381 aaaacttaat tatttatagt gttacttaaa aaatgtaaat tttttagagt aaaaatttgt
    16441 aatataacat ataacttaaa aataaaaagc agttagcctt ataaatgaag gttagctgtt
    16501 ttttttgtta aaaatatatt tacaaaataa gagatagtag cagtacaata taagtataag
    16561 gtaaaagtat tatatattag ggaggggtaa gtatgaaaaa tcaattaaat gaaaatcata
    16621 aaaaggttgt attttttgga gtaggtgctg taggagctac ttttgcagaa caatttttta
    16681 actctaaata tgattttaaa attctttgtg ataatgaaag aaagaaaaga tatttagaag
    16741 aaggatttat aataaatgga aaaaggtatg attttgatta tgtaactaaa gatgagtata
    16801 aacaagaggc tgattttata attataggtc taaaatataa taatttaaaa gaaaatataa
    16861 aagaattaga tggattagtt ggaaaaaata cagttataat gtctctgcta aatggagttg
    16921 atagcgaaga gataatagga gaaagatttg gaattgaaaa aatggtatat tcatatgtta
    16981 ccaatataga tgcaaagaaa atcaataata atattataca cactactaat gggataattg
    17041 tatttggtaa caaagataat agtgaagata gaaaaactaa tataataacc gaagtctttg
    17101 atgatgtaaa tatagaatat actttatcaa aagatattca acgagatatg tggtggaagt
    17161 acatggttaa tattggtgta aatcaaactt cagctatact tggtgcacct tatggagttt
    17221 ttcagagttc tgagcattta agagaattag caaaatctgc aatgagggaa gttgttgcta
    17281 tagcacaagc aaaaaacata tctcttacag aagatgatgt ggaacattca ttacatagaa
    17341 tactagaaca ttcaaaagaa ggaagaacat caatgcttca agatgtggaa gctcatagac
    17401 ttacagaagt agatatgttt tctaagaata tctgcaaact tggaaaaaaa tataacatac
    17461 ctactcctat aaatcagact ttcttttata tgataaaagt aattgaaagt agattttaaa
    17521 gggtctttgt aagaaatatt gataattatt gattttaaat tttattaatg ttatatacta
    17581 tttaagttac taaatatttt taactcttca atgaaaatag ttgctgcttt tgataaagtt
    17641 atatcttttg gaaatatata tgagaaatgt ctgttgtagc tgcttccaag ctctatgaca
    17701 gacagctcat tgtttagaac aggtaagctt gtcactagat tagagactat tgtaattcct
    17761 aaattatttc taacagattc tttgacagca taattacttc caaaaaccat catacttttt
    17821 ggaataattt ctttaacact taaaaacata tctaaataat ctctggttcc agaaccatcc
    17881 tccctgacta tccatttttg gttttgaagt ttatcaaaac taaaatcttt taataaatga
    17941 ctcttgtatg gaagtgctaa aaccatctta tcttcaaaaa aatattcttg aataaatgaa
    18001 ggggaagagc aagtgccttc aataagacca atgtctaaaa tataatcctt aacatgagaa
    18061 gatacgattg atgtattttt tataaaaact tctacatcta tgtcaggata ctttttagaa
    18121 aataatgcta gaaaatttgg taaaatatac tctccaatag ttaaacttgc accaatcttt
    18181 aagtgaccag ttatagcatc tgaaacttgg cttacatcat gataagtaat atttaaaaga
    18241 tttaatattt ctttagctct tttgtacaag atttgtccac tttctgttat aaaaatggtt
    18301 ttttgtttaa tggacctgtt tataagaaca actttaaaat aattttctag attttttatg
    18361 tgtttactta cacttggttg tgatagattt aaatattccc cagctttagt aaagtttttg
    18421 tattcaacaa cagctacaaa tgtttttaat tcttcaaaca attttttgtc tcctttacct
    18481 aatgaaattt tacatttttt atatactttc tacagataat tatactatat ataataacca
    18541 ttaaaaacat taatcattac gatattatat atttatttta cttatggtta ttacaattat
    18601 atactattgc tatgaagaaa actatattaa ttataaggta ggtatattat gttaaagaaa
    18661 aaaagcagtt tgacattaaa aactataagg gaaatattac cagggctgtt tgtatcagta
    18721 ttagtaggtt atattagtat atttatttct attttaattc ctaaggtagg ggcagcatca
    18781 atttctatat ttttaggtat gtttgtagga aacctatttt taaatcaaaa ggtatttcaa
    18841 aaaggttata aattttcaga gacagatttg ttatcatatt caatagtact attaggagca
    18901 acactcagtg tatctacctt aatagactta aaagtttcag gaattttatt tataatattg
    18961 caaatgacaa taacaataat agctgcactt tatataggta aaaaactagg atttgaagaa
    19021 aactttagat ttctaatggc aagtggaaat gcagtatgtg gttcttctgc aataggagca
    19081 acagttccag tggttaatgc gacagataaa gaaaagggaa ttgcagtaac tattgtaaat
    19141 gttactggta tatttcttat gtttttactt ccaataatat ctcaattttt atatagccat
    19201 gaacttgtaa aaacttcagc aatgataggt ggaactttgc aatcaattgg tcaggttgta
    19261 gcaagtggag caattgttgg agaaaatgta aaagatttag ctactatttt taagatagta
    19321 agagtaatat ttctagttgt tgttgtatta gtatttggac atatgaaaaa tcaatctaat
    19381 agggaaattc ttgaagaaga aaaggatgag ataaaaaaga aaaaagtgac tataccatgg
    19441 tatgtaattg gattctttat aacttgtgca cttttttcaa tgaacattat accacaagaa
    19501 gtatctgtgt tgtgcaaaga aataagtaac aaactagaaa taatagcttt agcagcaatt
    19561 ggattaaagg ttaatgtaaa agatttagtg aaacaaggga aagaagtttc tttatatggt
    19621 ttatttgtag gtacagtaca agttgtatca gctgtagttt taataaaaat atttatatag
    19681 cataaaaata gtatttgtaa tattaatttg attaattaaa ataatatgat actaataatt
    19741 tataatataa ataaa
//
LOCUS       DAGSRM010000070        19738 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.070, whole genome shotgun sequence.
ACCESSION   DAGSRM010000070 DAGSRM010000000
VERSION     DAGSRM010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19738
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.070"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            121..276
                     /locus_tag="MJE33_002629"
     CDS             121..276
                     /locus_tag="MJE33_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648558.1"
                     /translation="MKGILKELTAKLYSFFYFMFWGFYFSAGYFFYKNICTSFLVRPY
                     FSDFEHK"
     gene            406..1419
                     /gene="aroF"
                     /locus_tag="MJE33_002630"
     CDS             406..1419
                     /gene="aroF"
                     /locus_tag="MJE33_002630"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBY9648559.1"
                     /translation="MIVVLKMGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKVVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEDQIVSIAKDVKKSGAGFLRGGAFKPRTSPYAFQGLKYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            1441..2505
                     /locus_tag="MJE33_002631"
     CDS             1441..2505
                     /locus_tag="MJE33_002631"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBY9648560.1"
                     /translation="MEEKLLVNLKENSYNIFIKKNILKNIGEEIKKIYFGNKIFIVTD
                     ENIAKLYLDKVKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGISFIQIPTSLLAQVDSSVGGKVGVDLERGKNLVGSFY
                     QPKAVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFDKLLSLKNREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKYYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYEIELRDNKSIVDAISLDKKNLGDTLKIILL
                     KDIGESIIYNTTSKFFQEVR"
     gene            2505..3818
                     /gene="aroA"
                     /locus_tag="MJE33_002632"
     CDS             2505..3818
                     /gene="aroA"
                     /locus_tag="MJE33_002632"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBY9648561.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSMAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKKEDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLAFDGIKRFIGRGNLGKRPLDTYYEIFDRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTIKDFGVEI
                     INNNYKEFIIKGNQTYKARDYKVEGDYSQGAFYLSADAIGEDISILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNGTLIDASQCPDIIPVLSVVASLSIGRTTIIN
                     AGRLRIKECDRLHAINVELSKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFKMLGGRIDEWNMGK"
     gene            3796..4875
                     /gene="aroC"
                     /locus_tag="MJE33_002633"
     CDS             3796..4875
                     /gene="aroC"
                     /locus_tag="MJE33_002633"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBY9648562.1"
                     /translation="MSGIWGNNLKVSIFGESHGNAIGINIDGLPSGIELDLDNIDKEM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGRTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEIGAHIKKIK
                     NIEDMSFDYVNISKQQLSNLQTLELPLLDLSKEEAMKNTIIDAKNQGDSVGGIIECAV
                     VGINVGLGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGNQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSISRQQNTVNIKDKKDDILYIKGRHDP
                     CIVQRAIPVIEAVTAIGIFDLMKGR"
     gene            4878..6074
                     /gene="pheA"
                     /locus_tag="MJE33_002634"
     CDS             4878..6074
                     /gene="pheA"
                     /locus_tag="MJE33_002634"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBY9648563.1"
                     /translation="MEDLTRYRNEIDNIDKELIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRAVDNLDNKAYSQETVQFFNSIMEISRGLQKRLIDNDGEQKIEFSLDS
                     SNKKFDLSNVNRYKSLKDELNKKNILVGFSGKSGSFTEEALNKFFNKKISKKQFKEFE
                     DVFIALKNKEINYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLEDIKEIYSHPQGIGQCSEFLKQNSTWKLIPFNNTATSAELVRKLQDKTKAAIASKK
                     AASIYGLEIISPCINDIANNYTRFVVISNQIHIEEESNKMSVVFSVEHEAGKLYKVLG
                     YFAKNNINMTKIESRPMKNASWRYFFYIDFECSIYNSKVYDLLELIEHNTAYFKFMGV
                     YKNKTE"
     gene            6121..6930
                     /gene="aroE"
                     /locus_tag="MJE33_002635"
     CDS             6121..6930
                     /gene="aroE"
                     /locus_tag="MJE33_002635"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBY9648564.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRVFEILNIESAYKNFEISKEDIS
                     KLDGAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLRENMLYGYNTDY
                     FGLDSMFKMANIDVQGKVAVILGTGGASKAALTYFIDSGIEKLYVSTRKKDDKKLLNS
                     KAILIDYEELKHIKGDIILNATPVGMYPNVGISPVSKSIIQNFDILIDLIYNPGETEF
                     LRIGNSMGKKTCDGLYMLVGQAIKSQEIWQDTKIDNSILDVIYNELKLEFL"
     gene            6942..7475
                     /locus_tag="MJE33_002636"
     CDS             6942..7475
                     /locus_tag="MJE33_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBY9648565.1"
                     /translation="MINKTKEKLILIGMPGSGKTTIGKLLAKEYNCSFCDMDDYIIQI
                     SQKSIAELFSEGEDIFRNYETQACRELSISDKTVISTGGGVIKKDVNMEILKETGIII
                     FIDRPIQKILEDININSRPLLKNGKDRLYNLYNERINLYKKFSDIEILNDKSLNNAVY
                     NITNAVSENFKFDFKEK"
     gene            7496..8335
                     /locus_tag="MJE33_002637"
     CDS             7496..8335
                     /locus_tag="MJE33_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBY9648566.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKAK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNNNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKKGIDFASEQVFNGANYIITPTGRNNIKNLELVENLI
                     LEIGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEGRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGNRDNLLKVIENFESEVNLVKEAIFNNDKNKLIEYFKKSSIRREA
                     LEK"
     gene            8603..10084
                     /locus_tag="MJE33_002638"
     CDS             8603..10084
                     /locus_tag="MJE33_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBY9648567.1"
                     /translation="MKKNRLIAKNISIAFIVLFFFSVFTFFYVGNINRVLEYETNDII
                     TVTIAGWIILSFLFLGIIIYILYSKANSQKTIEKVAYTDFVTGYSNWRKFELDVTNLL
                     KKTSQNNKYAMVIFDIDKFKAINDIYGHKKGNLILKDIADTLNELTNINETFARVSAD
                     SFNILLTYNKKEDIINIIKKIMANNELVNLSFGIYEIKDKDLSVSVYSDRASLAKSSI
                     KNNSDVNFAFFNDKLREKLLLEDKIEKEMEYALESGQFVMYLQPKYNIKLDKFCGSEA
                     LVRWQYTEKEVIYPGDFIPIFEKNGFIRKIDMYILEQACKEIRSLFDKGISPLPISVN
                     FSRVDFFKKDFIENIVNICDRYKIPYSLIEIEITESSMFGDTDTLFNVSRNLQDIGFI
                     VAMDDFGSGYSSVNMLKNIPLNVIKLDRGFFVDDKDVDKSQIVIKSIVSLIKQLGIRV
                     VAEGIETRSQIEMLKKANCDIVQGYYFSKPLPIKEFEKLVYKI"
     gene            10117..13554
                     /locus_tag="MJE33_002639"
     CDS             10117..13554
                     /locus_tag="MJE33_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBY9648568.1"
                     /translation="MIRAKSLKNVLSIVLIFLFTIISFSDFAFKTNVQEEENSKINLR
                     EISLQSSNAIKIKLDSSLSFIKSVANMVSYFNTIDNSKIVPLLANISKESDFQRISIV
                     SPDGISHTSDGKIINVSKYQYFKKAIKGESNISEVVKSPIDGKDTFVIATPVFNKNNK
                     IIGVLYGSYYNDKLAEFIDITSFNSEGYIDIFESSGQFVLKSSHTNKLIKSSSNIYDI
                     FKKASFDDGYSYKKIYEDTKNNKSGFTSYEYKGENRYATYTPIGVNDWYVISVIPQYT
                     VRRNIDKINQLAFSLIFKVFLLFIMLIIYIIYKNNKSQKDILKANKEISSITNSIPGG
                     VQKCTLGETCNINFLSDGFIELFGYTREEIKTLFNNDFYSLIHKDDVERIKKEIFKQL
                     YKNKTIELEYRIITKNGNTVWVLDKGELITEPDGKEYFCCVTVDITRTKQLEQEMEQK
                     NLELEMISENVLGGIIITEFNDDFTIKYANSGYLSMIGYTREQLENELKYKACNLVFY
                     EDKKKALESIQHQLNTKGIAVYQHRIRRRNGNIIWVSIKGRLIVDDDGIEKGIWILND
                     ITDLKNAEQKLKINEERFRIALKQTSNTIFEYDIKTKSLLNTSKELDKYNIYKITKNV
                     PYSLVESGVIHKDFSDDFINMYENVINGAKKASCVVKMKLPDENYIWNKVTLTTIFDE
                     DGNPVRAIGIQEDISKQKEAEIRYNQEGKYHSAILSEAMITYEINVSKDTFIKGNESW
                     QKPFGIKPDNNYSQMVKTIANSIVHKDNKETFLNTFLCENLLDAYKNNVTEIKLEYRR
                     MNQENKMIWVICTMHLIKDSITNDIKALAYVKDIDKQKNEEIRLKLKAERDTLTGLYN
                     KGTTEKLINDFLNSEEASLGMHAFLIVDIDNFKSVNDNLGHVFGDKVLFEVSKNLKPI
                     FRKDDIVGRIGGDEFIVFLKNIQSFENIKKKAEKICDIFRNSYTENKKTYKISGTVGI
                     SFVPEHGTVFEELYKKADIALYYAKSKGKDKYALYNDCIYQNELENNISDNNIKLADK
                     CEKSKPVLKEILDSIDNYIYIVNPNTYELLFINNKVSRLDLEIKLGETCYKSLLKSDR
                     PCNNCPLNGLEENKHSKFKIETNTFGKNISIASWINCIDGEYNCLIECIDSSLYKNK"
     gene            13572..14318
                     /locus_tag="MJE33_002640"
     CDS             13572..14318
                     /locus_tag="MJE33_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBY9648569.1"
                     /translation="MLNSITLILGIIFTIYYFYLKIVFGGISFSEIFLVVGIVLIIYQ
                     IFKKKIKEKKVFYRVLKIIISIFLIVFVVTESLIIFYPKNSLESKSDYLLILGASVKK
                     TTPSTTLKGRLDTALKYLKINDNCYVVVSGGKGSGEKITEAKAMKDYLIKNGIDKNRI
                     IEEDKSTNTYENFKYSKLKIEEHSQGKLSDLKVKIVTTDFHVLRSKILAYRNGYKNTS
                     FYASKSKLSFVPTYYTREFFALWKTIIFDR"
     gene            complement(14267..14575)
                     /locus_tag="MJE33_002641"
     CDS             complement(14267..14575)
                     /locus_tag="MJE33_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBY9648570.1"
                     /translation="MNFFKENSTFYFATVEDNKPRVRPFGFIMDYEGKLYFGIVKYKT
                     SYKQLLVNPNVEISTTSKVGKWIRIKGIEKTRYEFISSLFIYYLSKMIVFHNAKNSLV
                     "
     gene            15379..19521
                     /locus_tag="MJE33_002642"
     CDS             15379..19521
                     /locus_tag="MJE33_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HBY9648571.1"
                     /translation="MATVSGQIVFDRNRSATIDSGDSGIANIPVVLQDIATGVRLVVL
                     TDASGNYVFTNVPNGNYRIVEAFGTTGGVPTPGNFATAIVGPVPIAEMPPISSVTSPP
                     IGSTNLDGTTPNTLLITVAGSDITNQNILNGPVIYTPIENILDSCVSVSNINLITDAD
                     NGTFGFFPPGTPSNTGPEIEPYPNVTPDFTYVVPDPTKFTPLDGEYTVQNIMTNAMSN
                     VIGAWWRIADHTTGNETGRMMVVNGFNPGAVFFKSTVQVTPNTNYLFSTWILNLFKVT
                     GYPPPQLGVRIFDQNNNILYQATLGAQIPVNTNAPEWKEVGTVINSQNNNQLTVEFFS
                     EGESAIGNDYAIDDVSLREIAVPTFTPIKTSNTETANVGDIITYTVTLDNTCDSPLTN
                     VFFRDLIPNGLEFIPGTVTVDGVSVPGVDPNTGFPLPDIGGGTGVEVTFDVVVESIPN
                     PNPTNNIATIDYSYTPVEGGIPNDFSVDSNPVPVEVISADIEVTKLSEPTIVNPGEEL
                     IYTIKVVNNGPFPSENVVLTDDVPASIVNPEYSLDGGVTFQPWTGSLNIGTLEVGETR
                     VIIIRGIVNPSTVGIITNTAVVSSTTADPNLNNNTSTIETEVNLLADILVMKTAEPNS
                     AVPGTLLRYTIQVENLGPANAENVILNDDIPASIINPEYSLDGGASFQPWNGSLNIGT
                     LNSGISLTVLIQGTVSLNSSEYIVNTATVSSTTPDPDLSNNISTIITPVNPQAGISII
                     KVADEDVAVPGEEFVYTIEIFNEGPSNATNVVLTDDIPDVILNPEYSLDGGATFQPWT
                     GSLNIGTVAPGQLIRIIIKGLVSSTATGDITNTAEVSADVPEPVTDSSTVTTPIVPSA
                     DIEVIKTSNMDTAVPGETFSYTITVTNLGPSAAQSIVLTDDIPDVILNPEYSLDGGVT
                     FQPWNGNLSIGTLDAGEIRSIIIRGTVSQTAVGTIINTATISSPTPDPNPDNNTSTDE
                     TDISPLADISVIKGNEPVAIPGGRFIYGIEIANAGPSFTENVTLTDNIPASILNPEYS
                     IDNGVTFQPWNGSLNIGTLDAGEIRNIIIRGTVSQTAIGTIINTATVSSTTPDPNLNN
                     NTSTSEAEVSSSADISVVKRSNQIVVVPGDVLDYTIEVRNAGPSTTQNVTLTDNIPAS
                     ILSPEYSIDNGVTFQPWNGSLSIGTLDAGEIRNIIIRGIVSQTAIGTIINTATVSSTT
                     PDPNPDNNTSTSEIDISSSADISVIKLANKTEACVGEQIDFVIVVSNAGPENAQNVTL
                     IDNVSDKLKKAVFSLDRGVSFQPWTGSLNIGTLPAGTLRVILLRGIIKSTCLDRLTNI
                     AEITSTTPDSNLNNNISRVQVEIKQCCCNDCCCNNCSNCCCNDCGCKDCCCNCCKNDF
                     WC"
BASE COUNT         8031 a         2179 c         3296 g         6232 t
ORIGIN      
        1 aagtaaataa aaaaatattt taaattgaaa atatttatag aaaatattga caattatatt
       61 ttctttgtta taattattaa acaagaagaa taataaatta taaaaatgga ggataatact
      121 atgaaaggaa tattaaaaga actaacagca aaactttata gcttttttta ctttatgttc
      181 tggggctttt attttagcgc tggctatttc ttttacaaaa atatatgtac ttcctttctg
      241 gtgaggcctt attttagtga ttttgagcat aagtaaattt tataaaaatg gttattttat
      301 atatttatat aagatttagt gcttgataga ttattttaga gaactcatta gtgagttctc
      361 ttttttattt taaatataaa attataatat tgggaggtag taaaaatgat agtagtatta
      421 aaaatgggag cagacaaaaa tgaagttaaa aaattaattg aggctattgg gagagaagga
      481 gtagaagtta atcccataga tggaacagaa ttaactgttt taggtttagt tggagatact
      541 agtaaaatag atgccaaaag aatagaagca aataaggtag ttgaaaaagt tatgcatgtg
      601 gtagagcctt ttaaaaaggc caatagaaaa tttcatccag aaccttcaat cataaatgtt
      661 aatggaatgg aaattggttc aaagaaaatt gctatgatag ctgggccttg ttctgttgaa
      721 acagaagacc aaatagtatc tatagctaaa gatgtgaaaa aatcaggtgc aggatttttg
      781 agaggtggag cattcaaacc aagaacatca ccatacgctt tccaaggatt gaaatatgat
      841 ggattggatt tattaaaaaa agcaaaagaa aaaacaggac ttcctatagt tacagaaata
      901 atgtcaacac aagatataga tatatttgaa gaaaatgtag atgtaattca agttggagct
      961 agaaacatgc aaaactttga tttattaaaa gaattaggta aaacaaataa aacaatactt
     1021 ttaaaaagag gattatctgc tacaattgaa gagtggctaa tgtcagcaga gtatataatg
     1081 gctggaggaa atgaaaatgt agttctttgt gaaagaggta taagaacttt tgaaacatat
     1141 actagaaata ctttggattt aagtgcaatt ttagctgtga agaaacttag tcatttacca
     1201 gttatagttg acccaagtca tgcagcaggt aaatcatgga tggttgactc tttatctaag
     1261 gcagcaattg cagttggagc agatggtcta ataatagaag ttcataatga cccagcacat
     1321 gcactttgtg atggaaaaca atcgattaag ccaaatgaat atgatgaatt gataagtgaa
     1381 ttaaagacta tcgcatcagc agttggtaga gaaatataat ctataaaagg atggttaaac
     1441 gtggaagaaa aactattagt aaatttaaaa gaaaatagct acaatatatt tattaaaaaa
     1501 aatatactaa agaatatagg agaagaaata aaaaaaatat attttggaaa taagattttt
     1561 attgtaactg atgaaaatat agcaaaatta tacttggata aagtaaaaaa taatttaagt
     1621 gaatctggat ttgaaattaa atctttaata ttaaaatctg gtgaagaaac aaaatcattt
     1681 aatactcttc ctaaaatata cgatagttta cttgacttta agttaactag aaaagatttg
     1741 attattactt taggaggagg ggtaataggt gatttaggtg gatttgcagc agcaactttt
     1801 ttaagaggta taagttttat tcaaattcca acatctttat tagcacaagt tgatagttct
     1861 gtaggtggaa aagttggagt tgaccttgag agaggcaaaa atttagtggg aagcttttac
     1921 caaccaaagg ctgtgtttat cgacccaaat gtattattat cacttccaga aaaattctat
     1981 agagatggaa tggcagaagt tataaaatat ggatgtataa aagacaaaac ttttttcgat
     2041 aaacttttaa gtttaaaaaa tagagaagaa gtaatgcaac atatagaata cattatacat
     2101 aaatgttgct ttattaaaaa aactgtagtt gaagctgatg aaaaagactt tggagataga
     2161 atgcttctaa actttggcca tacattaggt catgctattg agaaatacta caattttacg
     2221 ggttataccc atggagaagc tgttgccata ggaatgtata agataacttt agaaagtgaa
     2281 aagataggaa ttacaaaaaa agggacatca gaacttataa aatcaatttt aatgaattat
     2341 aatttgcctt atgagattga attaagagat aataaatcta tagtagatgc tatatctctt
     2401 gataaaaaaa atctaggtga tacattaaaa ataatacttt taaaagatat tggagagagt
     2461 attatatata atactacttc taaatttttt caggaggtaa gataatggga agtctaaaaa
     2521 tatatccaag taaattatct ggagatgtaa aaataccacc atcaaaaagt atggcacata
     2581 gggcagttat ttgttcatct ctaagcaatg gaaaaagtag aatttctaat atagattttt
     2641 ctgatgatat aatagctact ataagagcaa tgacatcatt aggagctatt atagaaaaga
     2701 aagaagatat tttagaaatt tcggggattt tttctaaaga aggtatttta aatagagaga
     2761 atcagttgaa tcaacctaaa ttgacaatag attgtaatga atcaggctct acattgagat
     2821 ttttagtacc tatatcttta gcatttgatg gtataaaaag gtttataggt agaggtaatc
     2881 ttggaaaaag accacttgat acttattatg agatatttga cagacagaat ataaaatata
     2941 gttacaaaga aaatcagtta gatttgatta tatcaggtaa gcttaaacca gatgaattta
     3001 gagtaaaagg aaatataagt tctcagttta tcacaggtct actgtttata ttgcctacgc
     3061 tagagagtga ttcaaagata ataattacta ctgagctaga atctaaagga tatttagatt
     3121 taactctttc aacgataaaa gattttgggg tagaaattat aaacaataac tataaagaat
     3181 ttattataaa aggaaatcaa acgtataaag cgagagatta taaagtagaa ggagattatt
     3241 ctcaaggagc attttattta tcagcagatg ctataggaga agatattagc atattagacc
     3301 ttaaagaaga ttctcttcaa ggggatagtg aagtagttga aattttatct aggatgggaa
     3361 tggaaatatt aagagaaggc aacaaaataa aaggaataac aaatggttta aatggtactt
     3421 tgatagatgc atctcaatgc ccagatataa tacctgtatt atctgtagta gcatccttaa
     3481 gtatagggag gactacaata attaatgctg gtaggcttag aattaaagaa tgtgatagac
     3541 ttcatgcaat aaatgttgag ttaagtaaac ttggagcaaa tatagaggag aaagaggata
     3601 gtttgataat cgaaggagta agcaagttaa atggtggagt agaggtgtgg agtcacaaag
     3661 accatagaat tgctatgaca cttgcaattg cttcttgtag atgtgataaa ccaataatat
     3721 taaaagattt tgagtgtgta tcaaaatctt atccacattt ttttaaagat tttaagatgt
     3781 taggaggtcg aattgatgag tggaatatgg ggaaataatt taaaagtatc aatttttgga
     3841 gaatctcatg gaaatgctat aggtataaat atagatggtc ttccttcggg gattgaactt
     3901 gaccttgata atattgataa agaaatgaag agaagagcac ctggaaaaaa ttctatatct
     3961 acttcaagaa atgaaagtga tataccagaa attctaagtg gatattttaa tggaagaact
     4021 acaggaacgc cattatgtgc aatcataaga aatagtgata ctcgttcaaa agattatgga
     4081 gagcttaaaa atttaatgag accaggacat gcagatttca caggtaatgt gagatattct
     4141 ggatttaatg actatagagg gggaggtcat ttttcaggta ggataacagc tccattagta
     4201 ttttgtgggg ctatatgtaa acaaatttta tcacaaaaag gaatagagat aggagcacac
     4261 ataaaaaaaa ttaaaaatat agaagatatg agttttgatt atgtaaatat ttcaaaacaa
     4321 cagttatcaa atttgcaaac tttggaacta cctttacttg atttatcaaa ggaagaagcc
     4381 atgaaaaata cgattataga tgcaaaaaat caaggagatt cagttggtgg tataattgag
     4441 tgtgcagtag taggtattaa tgtagggctt ggaaatccat tttttgactc tgttgaatct
     4501 actttatcac atttattgtt ttctgttcct gctgtaaaag gtgtagagtt tggtttaggt
     4561 tttgaacttg cagatatgta tgggtctcaa agtaatgatg aaatgtatta tgaaggaaat
     4621 caagttaaga gcaaaacaaa taacaatgga ggaattatag gagggattac tactggaatg
     4681 ccaataattt tcaaagtggc aattaagcca actccatcaa ttagcagaca acaaaacaca
     4741 gttaatataa aagataaaaa ggacgatatt ttatatatta aaggtagaca tgacccttgt
     4801 atagttcaaa gggctatacc tgtaatagaa gcagtaacag ccattggaat ttttgatttg
     4861 atgaaaggac gataataatg gaggatttaa cgagatatag aaatgagatt gacaatatag
     4921 ataaagagct tattcaatta tttgaaaaga gaatgaatac agttcttgaa attgccagac
     4981 ataaaatgaa aaataacact actatacttc aaaaagatag agaagaaaaa gtactaagta
     5041 gagctgtaga taatttagat aataaagcat attcacaaga aacggtacag ttttttaatt
     5101 ctataatgga aattagtaga ggattacaaa aaagattgat agataatgat ggtgagcaga
     5161 aaatcgagtt tagtttagat tcatcaaata agaaatttga tttgtcaaat gtaaatagat
     5221 ataagtcttt aaaagatgaa ttaaataaaa aaaatatcct agtagggttt tcaggaaaat
     5281 caggttcatt tacagaagaa gcactaaata aattcttcaa caaaaaaata tcaaaaaaac
     5341 aatttaaaga atttgaagat gttttcatag cacttaaaaa taaagaaata aactatggaa
     5401 taatccccat agaaaattca tctacaggag ctatctctga aacttatgac cttttgagaa
     5461 agtacggttt ttatatagtt ggagaagaat gtataaaaat agaccaaaat ttaataggga
     5521 taaaagacac aaagcttgaa gatataaaag aaatatattc tcatccacaa ggtataggac
     5581 aatgtagtga atttttaaaa caaaatagta catggaaatt gataccattt aataacacag
     5641 caacaagtgc agaacttgta aggaaacttc aagataaaac aaaagcagca attgcaagca
     5701 aaaaagctgc aagtatttat ggattagaaa taataagtcc ttgtataaat gatattgcaa
     5761 acaactatac aagatttgta gtaatcagta atcaaataca cattgaagaa gaatcaaaca
     5821 aaatgagtgt agtattttct gttgaacatg aagcaggaaa actttataaa gtacttggat
     5881 actttgctaa aaataatata aatatgacaa aaatagaatc aagacctatg aaaaatgcct
     5941 catggagata tttcttttat atagattttg agtgttctat atacaattct aaagtgtacg
     6001 atttattaga attgatagaa cataatacag catattttaa atttatggga gtatataaaa
     6061 ataaaactga atagataaga taaattttta tttaatttat aattattagg aggtgtaagt
     6121 atgaattttt tcggtctagt aggtgaaaaa ctctctcaca gtgtttcacc tcaaatccac
     6181 aagagagtat tcgagatatt gaatattgaa agtgcttata aaaactttga aatttcaaaa
     6241 gaagatatat ctaagctaga cggtgctata aaattattag gaatacaagg agtaaatgtt
     6301 actgtaccat ataaggagcg tattatgaaa tatcttgatt ttatatcacc agaggcaaaa
     6361 cgtataggag cagttaatac aattttactc agagaaaata tgttatatgg ttacaataca
     6421 gattactttg gtcttgatag tatgtttaaa atggctaata ttgatgttca aggaaaagta
     6481 gcagttatat taggaactgg aggtgcttct aaagctgctc ttacatattt tattgattct
     6541 ggtatagaaa agctttatgt ttcaactaga aaaaaggatg ataaaaaatt attaaatagt
     6601 aaagctatat taatagatta tgaagaactt aaacatataa aaggagatat aatattaaat
     6661 gctacaccag taggtatgta ccctaatgta ggaatttcac cagtgtcaaa atctattata
     6721 caaaattttg atatattaat agatttaatt tacaatcctg gagaaacaga gtttttaaga
     6781 attggtaata gtatgggtaa aaaaacttgt gatggattat atatgctggt ggggcaagca
     6841 ataaaatctc aggaaatttg gcaagatact aaaatagata atagtatttt agatgttata
     6901 tataatgaac taaaattaga atttttgtaa gggagattgt tatgataaat aaaacaaaag
     6961 aaaaattgat attaataggt atgccaggta gtggaaaaac tacaattggt aaacttttgg
     7021 ccaaagaata taattgtagt ttctgtgata tggatgatta tataattcaa atatctcaaa
     7081 aaagtatagc agagttgttt tcagaaggag aagatatctt taggaactat gagactcaag
     7141 catgtagaga actttctata agtgataaaa cagtgatatc tacaggtgga ggtgtaataa
     7201 aaaaggatgt taatatggaa atactaaaag aaacaggtat aataattttt attgatagac
     7261 ctatacaaaa gattttggaa gatataaata ttaattcaag accattatta aaaaacggaa
     7321 aagatagatt gtataatctt tacaatgaga gaataaatct ttataaaaaa ttttcagata
     7381 tagagatttt aaatgataaa tcattaaata atgctgtata taatattaca aatgcagtca
     7441 gtgaaaattt taaatttgac tttaaagaaa aataatctag ggggaagtaa aaataatgaa
     7501 tattgttatt gtaggactag gagttatagg aggctctttt gctaaagcat taaaaaaagc
     7561 tggatatgaa aatgtatttg gagtagatgt agatttagaa actttaaaaa aagcagaaaa
     7621 agcaaaaatc ataaaaaaag gatgtactac aggtaaagag ttatttaaaa aagctgattt
     7681 aattatacta tctatatatc ctagattagt tgtagatttt ctaaataata ataaaaactt
     7741 ttttaaaaaa gggactataa ttactgatac tacaggaata aaagaaacac ttatcaatga
     7801 tgtgttgcaa attattccag atgatataga ttttatattt ggacatccaa tggcaggtag
     7861 ggaaaaaaag ggtattgatt ttgcaagtga acaagttttt aatggcgcaa attatataat
     7921 tactccaact ggcagaaaca acataaaaaa tttagagtta gttgaaaatt taattttaga
     7981 gattggattt aaaagagtaa aaaagttaac ttcacaaaaa catgatgaga ttatagcttt
     8041 tacttctcag ttacctcatg ttatggcagt tgctcttata aatagtgatg aagaaggtag
     8101 agatacgggt aaatttattg gagatagtta tagagattta actagaattg caaatatgaa
     8161 tgaagactta tggagtgaac tttttttagg aaatagagat aatttactaa aagttataga
     8221 aaactttgaa tcagaagtaa atttagtaaa agaagctata tttaacaatg ataaaaataa
     8281 attaatagag tattttaaaa aatcatcaat aagaagagaa gctttagaaa aataaatatt
     8341 aatatttaag ctatatatag atgggtataa atataagcga tagtgtattt aattgtatta
     8401 ataaatatgg attcaatata attaaatatg ctattgatat atttgtaccc ttaactattg
     8461 ttatctttta aatgataatg atatttattt aaatactctt tattaacaat ttataaaact
     8521 taattgtaaa aaatacttat taatagtaaa ataaagtgaa tcaattatat tataattgct
     8581 aaagagagtg aggtgtagct atttgaaaaa aaatagatta atagcaaaga atatatcaat
     8641 agcatttata gtactattct tttttagtgt atttacattt ttttatgtag gtaatattaa
     8701 tagagtgtta gaatatgaaa caaatgacat aataacagtt acaattgctg gatggattat
     8761 actttcattt ttatttttag gtattataat ttacatatta tattcaaaag caaatagtca
     8821 aaaaactata gaaaaagtag catatacgga ttttgtaaca ggttattcca actggagaaa
     8881 gtttgaatta gatgttacaa atttattaaa gaaaacatca caaaataata aatatgcaat
     8941 ggtcatattc gatattgata aatttaaagc aattaatgat atatatggtc ataaaaaggg
     9001 aaatttaata ttaaaagata ttgcagatac tttaaatgaa ttgactaata tcaatgaaac
     9061 ttttgctcgt gtaagtgctg atagttttaa tatattatta acatataaca aaaaagagga
     9121 tataataaat attatcaaaa agattatggc aaataatgaa cttgtaaact tatcttttgg
     9181 aatttatgaa atcaaagata aggatttatc tgtaagtgta tatagcgata gagcttcact
     9241 tgcaaaatca tcaataaaaa ataatagtga tgtaaatttt gcatttttta atgataaatt
     9301 aagagagaaa ttattacttg aagataagat agaaaaagaa atggaatatg ctcttgaaag
     9361 tgggcaattc gtaatgtatt tacaaccaaa atacaatatt aaattagata aattttgtgg
     9421 aagtgaagcc ctagtcagat ggcaatatac agaaaaagag gtcatatatc caggtgattt
     9481 tattcctata tttgaaaaaa atggatttat tagaaagatt gatatgtaca tattagaaca
     9541 agcttgtaaa gaaatccggt ctttatttga taaaggaatt tcacctttac ctatatctgt
     9601 aaacttctca agagttgatt tttttaaaaa agattttatt gaaaacatag taaatatatg
     9661 tgatagatat aaaataccat atagtttaat agaaattgaa attactgaat ctagtatgtt
     9721 tggagatact gatactctat ttaatgttag tagaaaccta caggatattg gctttatagt
     9781 tgcaatggat gattttggct cgggatattc ttctgtaaat atgcttaaaa atataccatt
     9841 aaatgtaata aaattagata gaggattttt tgttgatgat aaagatgttg ataagagtca
     9901 aattgttata aaaagcatag tttctctcat taagcaatta ggaataaggg ttgttgctga
     9961 aggaattgaa acaagaagtc aaatagaaat gttaaaaaaa gcaaattgtg atattgttca
    10021 aggatattat ttttcaaaac cattaccaat taaagaattt gaaaaattag tctataaaat
    10081 atagtcagcg agtcttaaat gggaggtggt gattaagtga taagagcaaa atcgttaaaa
    10141 aatgtactga gtattgtttt aatattttta tttacaataa tttctttttc tgattttgca
    10201 tttaaaacta atgtccagga agaagaaaat agtaaaatta atttaaggga aatatcccta
    10261 caaagttcca atgctataaa aataaaatta gatagttctc tatcctttat taaaagtgtg
    10321 gcaaatatgg tttcttattt taatactata gataactcaa aaatagttcc attattagct
    10381 aatatatcaa aagaatctga ctttcaaaga atctctattg tatctcctga tggaataagt
    10441 cataccagtg atggcaaaat tatcaatgtg tcaaagtatc agtattttaa gaaagctatt
    10501 aaaggagaaa gtaatatatc tgaagttgtg aaatctccaa ttgatggaaa agatactttt
    10561 gttatagcta ctcctgtatt taataagaat aacaaaataa ttggtgtttt atacggttct
    10621 tactacaatg ataaattagc tgaatttata gatataacaa gttttaatag tgaagggtac
    10681 atagatatat ttgaatctag tggtcaattt gttttaaaat cttcacatac aaacaaactg
    10741 attaagtcaa gtagtaacat atatgatata tttaaaaaag cttcttttga tgatggttat
    10801 agctataaaa aaatatatga agatacaaaa aataacaaga gtggctttac ctcatatgaa
    10861 tacaaaggag aaaatcgtta tgctacttat acacccattg gtgtaaatga ctggtatgta
    10921 atttctgtga taccacaata tactgtaaga agaaacattg ataaaataaa tcaattagca
    10981 ttttctctta ttttcaaggt attcttattg tttattatgc ttataatata tattatttat
    11041 aaaaacaata aatctcagaa agatatacta aaagctaaca aagaaatctc atctattaca
    11101 aatagcattc ctggaggagt acaaaagtgt accctgggtg aaacatgcaa tattaatttt
    11161 ttaagtgatg gatttataga gttgtttggt tatacaagag aagaaataaa aacattgttt
    11221 aataatgatt tttatagttt aatacataaa gatgatgttg aaagaataaa aaaagaaatt
    11281 tttaaacaac tttacaaaaa taaaactatt gaattagaat ataggattat aacaaaaaat
    11341 ggtaatacag tatgggtttt agataaaggg gagcttataa cagaacctga tggaaaagaa
    11401 tatttttgtt gtgtaactgt agatattact agaacaaaac aattagaaca agagatggaa
    11461 caaaaaaatc tagaacttga aatgatatca gaaaatgttt taggtggtat aattataaca
    11521 gaatttaatg atgattttac tataaaatac gctaactctg gttatctttc aatgattgga
    11581 tatacacgtg aacaattaga aaatgaactt aaatacaaag cttgtaatct tgttttttat
    11641 gaggataaaa agaaggctct tgaaagtata caacatcaat taaacacaaa aggaatagct
    11701 gtatatcaac atagaataag aagaagaaat ggtaatatta tttgggtttc tataaaagga
    11761 aggcttattg ttgatgatga tggtattgaa aaaggtattt ggattctaaa tgatataaca
    11821 gatttaaaaa atgcagaaca aaaacttaaa ataaatgaag aaagatttag aattgcatta
    11881 aagcaaacat caaacactat atttgagtat gatataaaaa caaagtcact tttaaatact
    11941 agtaaagagc ttgataaata taatatttat aaaattacta aaaacgttcc atattctcta
    12001 gtagaatcag gagttattca taaagatttt agtgatgatt ttataaacat gtatgaaaat
    12061 gtaataaatg gagcaaaaaa agcaagttgt gtagttaaga tgaaacttcc agatgaaaat
    12121 tatatatgga ataaagttac cttgactact atttttgatg aagatggtaa tcctgtaaga
    12181 gcaattggta tacaggaaga tataagcaaa caaaaggaag cagagataag atacaatcaa
    12241 gaaggtaagt atcattcagc tatattgtcg gaagcaatga taacttatga aataaatgta
    12301 tcaaaggata cttttatcaa aggaaatgaa agttggcaaa aaccatttgg aattaagcct
    12361 gacaataact attctcaaat ggtaaaaact atagcaaata gtattgtaca taaagacaat
    12421 aaagaaacat ttttaaatac atttttatgt gaaaacttat tggatgcata taaaaataat
    12481 gtgactgaaa taaaattaga atatcgaaga atgaatcaag aaaataaaat gatttgggtc
    12541 atttgtacaa tgcacttaat aaaagattct attacgaacg atataaaagc acttgcttat
    12601 gttaaagata ttgacaagca aaaaaatgaa gaaattagat taaaattaaa agctgaaaga
    12661 gatactctta caggattata taataaaggt actacagaaa aattgattaa tgatttcttg
    12721 aactctgagg aagcaagttt aggaatgcat gcatttttaa ttgttgatat agataatttt
    12781 aaatctgtga atgataattt aggacatgta tttggagata aagtattatt tgaagtttct
    12841 aaaaacctaa aacctatatt tagaaaagat gatatagtag gtagaattgg cggagatgaa
    12901 tttatagtat tcttaaaaaa tattcagtct tttgagaata tcaaaaagaa agcagaaaaa
    12961 atatgcgata tatttagaaa ttcttataca gaaaataaaa agacatataa aatatctgga
    13021 acagttggaa tttcatttgt accagaacat ggaactgtat ttgaagaact ttataaaaaa
    13081 gcagacatag cactttatta tgcaaagagc aaaggaaaag ataagtatgc actttataat
    13141 gattgtatct accaaaatga acttgaaaat aatataagtg ataataatat aaaattagca
    13201 gataaatgtg agaaatccaa accagtactt aaagaaattc ttgattctat tgataattat
    13261 atctatatag tgaatccaaa tacttatgaa cttcttttta tcaataataa ggtgtctcgt
    13321 ttggatttag agataaaatt aggagaaacc tgttataaat cactattaaa atctgataga
    13381 ccatgtaata attgccctct taacggatta gaagaaaata agcattcaaa attcaaaata
    13441 gagacaaata catttggtaa aaatatcagt atagcaagtt ggattaattg catagatgga
    13501 gaatacaact gtcttataga gtgtatagat tcttctttat acaaaaataa ataaaaaagg
    13561 actatgaaga tatgttaaat agtataactt tgatacttgg tataatattt acaatttact
    13621 atttttatct caaaatagtt tttggaggaa tttcatttag tgagattttc ttagttgtag
    13681 gaattgtact aattatctat caaatattta agaaaaaaat taaagaaaag aaggtttttt
    13741 atagggtgtt aaaaataata atcagcattt ttttaatagt atttgtagtt actgagagtc
    13801 taattatttt ttatcccaaa aatagtttag aatcaaaaag tgattatctc ttaattttag
    13861 gagcttctgt aaaaaagact acaccaagta caaccctgaa gggtaggtta gacactgcat
    13921 tgaaatacct aaaaattaat gataattgtt atgtggtagt atctggtggt aaaggaagtg
    13981 gagaaaaaat aactgaggca aaagctatga aagattatct tataaaaaat ggtatagata
    14041 aaaataggat aatagaagag gataaatcca cgaatactta tgagaatttt aaatattcaa
    14101 aattgaagat agaagaacat agtcaaggaa aactaagcga tttaaaagta aaaatagtaa
    14161 caacagattt tcatgttctc agaagtaaaa tattagcata tagaaatgga tataaaaata
    14221 cgtcttttta tgctagtaaa tcaaaacttt catttgtacc aacttactat acaagagaat
    14281 tttttgcatt atggaaaaca atcatttttg atagataata aataaaaaga ctagatataa
    14341 actcatatct agtcttttct attcccttaa ttctaatcca ttttccaact ttacttgttg
    14401 tagaaatttc aacattggga tttacaagta attgcttata agaagtttta tattttacga
    14461 ttccaaaata taattttcct tcataatcca ttataaatcc gaatggtctt actcttggtt
    14521 tattatcttc aactgtagca aaatagaatg ttgagttttc tttaaaaaaa ttcattattt
    14581 tttcatgatt aaatcctctt tgtatcttat actaatataa tgaatataaa taaaatattc
    14641 ataatactaa tataaatcga tataaaaaca attacaatat tatattgatt attcaaaatt
    14701 atatataaaa ttcagaaaaa aattttagtt tacataataa aaataaagtc aactaaaatt
    14761 attgatttta gattaaagta ccggattaag catttaagta gattttaagc ttgatttgat
    14821 tagaaaggta caaactatca ccgaaaaaaa ttaaacccat ttaaaaagca atataaaagc
    14881 ttgttatttt tgagaaaaat agaccctcta aactcaatta gattaaaaat aaaatacaga
    14941 aaattatatc tctaaatatc ttaaataatt aagtttaaat tttacgatat tttgtttgat
    15001 gatttataat ataaaagcat actcttaatc ttcaaaattt aaaaagagta aaaaataaca
    15061 aaaaaatcac tgacatttca ctgacaaatt gtaattttgt tattataaat acattgaaaa
    15121 tacttgatta ttgaaaaatg gcataaaata atacatttta tgccatttgc tattatttga
    15181 tagtttttaa cttatgacat aagatatttt ttaacatata caataaaata taaaaaaatt
    15241 gatattgcaa ataaaaaaaa atacatatta gatgttatag aaaaaaagcg cacttatagg
    15301 cttaaaatgt cacataaaag agttttaaag aataatatat tatgaacaca aattttgtaa
    15361 tcacaaagga gtgataatat ggcaacagta tcaggacaaa tagtttttga tagaaataga
    15421 agtgctacca ttgattctgg agactcagga atagccaata taccagttgt attacaagat
    15481 atagcaactg gagttagact tgtggttctc acagatgcat caggaaatta tgtttttact
    15541 aatgttccaa atggtaacta tagaattgta gaagcttttg gaactacagg aggagtacca
    15601 acaccaggaa attttgcaac agcgatagta ggtccagttc caattgcaga aatgccacct
    15661 attagttctg tgacaagtcc tcctattggt tcaacaaact tagatggtac aacacctaac
    15721 acactattaa taacagtagc aggttcagat ataacaaatc aaaatatatt aaatgggcct
    15781 gtgatttata cacctataga gaatatattg gattcttgcg tatctgtatc taatataaac
    15841 cttattacag atgcagataa cggaacattc ggtttttttc caccaggtac accaagtaat
    15901 acaggacctg aaatagaacc ttacccaaat gtgacgccag attttacgta tgttgtacca
    15961 gacccaacta agtttacacc tttagatgga gaatatacag tacaaaatat tatgacaaat
    16021 gcaatgagta atgttatagg agcttggtgg cgtattgctg accatactac aggaaatgaa
    16081 acaggaagaa tgatggtggt aaatggtttt aatcctggag ctgtattttt taaatcaaca
    16141 gtacaagtta cacctaacac taattattta tttagcactt ggattttaaa cctttttaag
    16201 gtaacaggat atccaccacc acagctaggt gtaagaatat ttgaccaaaa taataatata
    16261 ctatatcaag caacattagg agctcaaatt ccagttaata caaacgcacc agaatggaaa
    16321 gaagtaggta ctgtgattaa ttctcaaaat aataatcaac ttacagtaga attttttagt
    16381 gaaggagaaa gcgccattgg aaatgactat gctatagatg atgtttcttt aagagaaata
    16441 gctgttccaa cttttactcc aataaaaaca agtaacacag aaactgctaa tgtaggtgat
    16501 ataatcacat atacagttac attagataat acttgtgata gccctttaac taatgtattt
    16561 tttagggatt taattccaaa tggtttagag tttatacctg gaactgtaac ggttgatgga
    16621 gtatctgtac caggagtaga ccccaataca ggtttcccac ttccagatat tggaggagga
    16681 acaggggttg aagtaacttt tgatgttgtg gttgaaagta ttcctaaccc aaatcctact
    16741 aataatatag ctactataga ttactcctat acacctgtag aaggcggtat accaaatgat
    16801 ttttcagtag attctaatcc agttcctgtt gaagtaatct cagcagatat tgaagttaca
    16861 aaattatctg aacctactat agtgaatcca ggagaggaat taatatacac tataaaagta
    16921 gtaaataatg gaccattccc atctgaaaat gtggttttaa cagatgatgt accagcttct
    16981 attgtaaatc cagagtattc attagatgga ggagtaactt ttcaaccatg gactggaagt
    17041 ttaaatatag ggacattaga agtaggagag acaagagtta ttataattag gggtattgta
    17101 aatccatcaa cagtaggaat tataacaaat acagctgtgg tatcttcaac tacagcagac
    17161 ccaaacctta ataataatac atctacaata gagacagaag tgaatttatt agcagatatt
    17221 ttagtaatga aaacagcaga acctaattca gctgttccag gtactttatt gagatatact
    17281 attcaagtag aaaacttagg accagcaaat gctgaaaatg taatattaaa tgatgatatt
    17341 ccagcttcta ttataaatcc agaatattca ttggatggag gagcaagttt tcaaccatgg
    17401 aatggaagtc ttaatatagg aactttaaac tctggaattt cattaacagt tttaattcaa
    17461 ggaacagtta gtttaaattc ttcagaatat atagtaaata cagcaacagt ttcttcaact
    17521 acacctgacc cagatttaag caacaatata tctaccatta ttacaccagt taacccacaa
    17581 gcaggtattt ctattataaa agtagctgac gaggatgtag ctgttccagg agaagaattt
    17641 gtatatacaa ttgaaatttt taatgaagga ccatctaatg ctacgaatgt agttttaaca
    17701 gatgatatac cagatgttat tttaaatcca gaatattcat tggatggagg agcaactttc
    17761 caaccatgga ctggaagtct taatataggt acagttgcac cagggcaatt aataagaatt
    17821 atcataaagg gattagtaag ttcaactgct acaggagata ttactaatac agcagaagta
    17881 agtgcagatg taccagaacc agttacggat tcatcaacag tgacaactcc tatagttcca
    17941 tcagcagata tagaagtaat aaaaactagc aatatggata cagcagttcc aggagaaact
    18001 ttcagttata ctataacagt tacaaattta ggaccatctg ctgcacaaag tatcgttttg
    18061 acagatgata taccagatgt tattttaaat ccagagtatt cattggatgg aggagtaact
    18121 ttccaaccat ggaatggaaa tcttagtata ggcactttgg atgctggaga aataagaagt
    18181 attataataa gaggaacagt gagccagaca gcagtaggta caattataaa tacagcaaca
    18241 ataagttcac ctacacctga cccaaatcca gataataata cttcaacaga tgaaacagat
    18301 ataagtccat tagcagatat ttctgtgata aaaggcaacg aaccagttgc tataccaggg
    18361 ggaagattta tttatggaat tgaaattgca aatgcaggac catcttttac agagaacgta
    18421 actttaacag ataatatacc agcttctatt ttaaatccag agtactcaat agataatgga
    18481 gtgactttcc aaccatggaa tggaagcctt aatataggta ctttagatgc tggagagata
    18541 agaaatatca taataagggg aacagtaagt caaacagcaa taggcacaat tataaataca
    18601 gcaacagtaa gttcaactac acctgaccca aatctaaata acaatacttc aacatcagaa
    18661 gcagaggtaa gttcatcagc agatatttct gtagtaaaac gaagcaatca gattgttgtt
    18721 gtaccaggag atgtactaga ctatacaatt gaggtgagaa atgcaggacc gtctactact
    18781 cagaacgtaa ctttaacaga taatatacca gcttctattt taagtccaga atattcaata
    18841 gataatggag tgacttttca accatggaat ggaagtctta gtataggcac tttagatgct
    18901 ggagagataa gaaatattat aataagggga atagtaagtc aaacagcaat aggtacaatt
    18961 ataaatacag caacagtaag ttcaactaca cctgacccaa atccagataa taatacttca
    19021 acatcagaaa tagatataag ttcatcagca gatatttctg taataaaatt agcaaataaa
    19081 acagaagcat gtgtaggaga gcaaatagat tttgtaatag tagtatcaaa tgcaggacca
    19141 gaaaatgctc aaaatgttac tttaatagac aatgtttcag ataagcttaa gaaagcagta
    19201 ttttcacttg atagaggtgt aagtttccaa ccatggactg gaagtcttaa tataggtaca
    19261 ctaccagctg gaactttaag agttattcta ttaagaggaa ttataaaatc aacatgtctt
    19321 gatagattaa caaatattgc agaaattact tcaacaacac cagattcaaa ccttaacaat
    19381 aatatatcaa gagtgcaagt agaaataaaa caatgttgtt gtaatgattg ctgttgtaac
    19441 aactgtagta attgttgttg caatgattgt ggttgtaaag attgttgctg taattgttgt
    19501 aaaaatgact tttggtgttg attttataaa ataattttgt ataccttaaa ttaaactgaa
    19561 ataaaaaata aattcacata tgagtaaaat attttataaa tattaataaa ttttctatct
    19621 ttgatatgga ccacattcgt aatactagtt attaaatgaa gtggtccttt ttaattcatt
    19681 aaaattattt attctaatta aattttaaat gtattaaata attttaattt tttataaa
//
LOCUS       DAGSRM010000071        19494 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.071, whole genome shotgun sequence.
ACCESSION   DAGSRM010000071 DAGSRM010000000
VERSION     DAGSRM010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19494
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.071"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            312..2357
                     /gene="bclA1"
                     /locus_tag="MJE33_002643"
     CDS             312..2357
                     /gene="bclA1"
                     /locus_tag="MJE33_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA1"
                     /protein_id="HBY9648572.1"
                     /translation="MRNIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFFKVRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYYNLRTITWND
                     RVSMENIRGYLPVGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNYPYQILEFTS
                     SRGCNKPYILVTFEDRIIDNCYPKCECLPIRITGPMGPRGATGSTGPTGVTGPTGSTG
                     ATGSIGPTGPTGNTGATGSIGPTGVTGPTGSTGATGSIGPTGVTGPTGNTGVTGSIGP
                     TGATGPTGNTGVTGSIGPTGVTGPTGNTGEIGPTGATGPTGVTGSIGPTGATGPTGEI
                     GPTGATGATGVTGSIGPTGATGPTGATGVTGEIGPTGEIGPTGATGPTGVTGSIGPTG
                     ATGEIGPTGEIGPTGATGPTGVTGSIGPTGATGPTGATGEIGPTGATGPTGNTGVTGE
                     IGPTGATGPTGNTGVTGEIGPTGATGPTGVTGEIGPTGNTGATGSIGPTGVTGPTGAT
                     GSIGPTGATGATGVTGPTGPTGATGNSSQPVANFLVNAPSPQTLNNGDAITGWQTIIG
                     NSSSITVDANGTFTVQENGVYYISVSVALQPGSSSINQYSFAILFPILGGKDLAGLTT
                     EPGGGGVLSGYFAGFLFGGTTFTINNFSSTTVGIRNGQSAGTAATLTIFRIADTVMT"
     gene            complement(2596..4194)
                     /locus_tag="MJE33_002644"
     CDS             complement(2596..4194)
                     /locus_tag="MJE33_002644"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HBY9648573.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDIDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILAVTDIIKEGSYLIYKAEDSLIANAFGVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
     gene            5072..7714
                     /gene="adhE"
                     /locus_tag="MJE33_002645"
                     /gene_synonym="adhC"
     CDS             5072..7714
                     /gene="adhE"
                     /locus_tag="MJE33_002645"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBY9648574.1"
                     /translation="MEKKEKVVEKSNVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVGKVVIIDGSLNARIVGQPAHVIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPERVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAREGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTAASGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGSTDQEKVDNLIKAIDELKAKVGLPKTIKEAGVEESKFLERLDAMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
     gene            complement(8074..8415)
                     /locus_tag="MJE33_002646"
     CDS             complement(8074..8415)
                     /locus_tag="MJE33_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648575.1"
                     /translation="MLLILGNLNLNLDFDYRIIREENEDVDIFIDINYRSLDIDTDGS
                     NLFNSRIQFPFVRALILRLNKNNQCMTIHLLRDIDLFSAFANFEVDYTDSIINIKNQN
                     EKVILNKSIKK"
     gene            complement(8442..9113)
                     /locus_tag="MJE33_002647"
     CDS             complement(8442..9113)
                     /locus_tag="MJE33_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9648576.1"
                     /translation="MPILETINLGKIYGKKETSVHALKNANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSNGTIKVDGKDICCLSDKELARYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKIDKEYIDDLINLLGLNERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKADRIISIIDGE
                     LKLTL"
     gene            complement(9125..11701)
                     /locus_tag="MJE33_002648"
     CDS             complement(9125..11701)
                     /locus_tag="MJE33_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBY9648577.1"
                     /translation="MKDNLKIVLRYIKSYKARSLAIVVSIILATSLVVGIGTLSRSAQ
                     QADVDRLKRELGTDHVYFKNIKKNQLKSIKSSKDIKNLGITSYYASTDVNEKLPINIL
                     YTNKNYLTSHSKLIKGRFPKASNEVVVEKWILNSMGLKPEINQNLTFKLYQKENPETF
                     KVVGILEDRFIEKKKGQCEIFSNLNESELNDFTTYIEFNEGSDINTNIDNIIKNAMLD
                     EKSIGRNSMLIESTMKNGTLDNSSKYVAIAMSLFAGIVIYSIYVISIYQRVQEYGVLR
                     AIGSTNFRIFKLMLYELFILALIAMPIGICIGMGGAQIFNRSVGNIQFEGNINVTPFV
                     IPDKIILLSIACTILIILIISFFTYLKIRRISPIDAIRKTFGADKNVTKVNYLISKLT
                     LNMSVTKSISAKNIFRNKKGFIIIVLSMSIGGIIVIKDNYKYSFLDVQNKSTQEQTYM
                     NADFILSNVYLNEIGNNTSFKDIKGFNDNQIDKIKNIDGIDKVKTASILNTRIEVNKI
                     NRLDYYEAINSTPYYKLNPLFIKNKTTGKYTLKQKLKGYNDEMIKSLEKYLVSGNINL
                     KKMKKENLAIVYVPEISKTQKYTKTFTPGEGTPAVDIKVGDTIKVKYPKGGMDTGLYE
                     SLKDNYEYLEYEFKVGAIISYPFADNGMYSGDDGVDVITSSEYLKKLTGVDKYNLVYA
                     DLNENANKKKINNLLGKIGSEVPGTTTVDMMVEKENSDKMTSRTMIYSYGIVAIMFII
                     SVLNIINNISYNLTSRTSEFGMLRAIGISERGFKNMILYEGILYGVLSSIITIVSGII
                     IQFKMYYMQGFVSYGLGFSIDYKIYILVVVANIIVGILATYIPSRKINKISIVEAINI
                     TE"
     gene            11909..12844
                     /locus_tag="MJE33_002649"
     CDS             11909..12844
                     /locus_tag="MJE33_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBY9648578.1"
                     /translation="MDNNFVFPIVILTLFIFSNLIMNKYKFYKELDILKRGILTFFIS
                     CFALILFMEGTKYIQLRLGYSFLLEGVLNIILFLVLIILSFYTGFKVIRLINNNNEQE
                     YKLQKLDSKINHKDNIIIKLRSQKHDYLKHLQVVYSLLNTGLDEDAKEYIMNISNSFK
                     SKNSRYGKVSYLDAIASLKYEECLEKDISFDVYIQDFMDNLEIEPNDMSSVLLNIIDN
                     AIESLSTIKKEKKYIRLHAYEDEFQYIIAIKNNGPKIHNVESIFWNGFSTKEGQDRGY
                     GLHLVKNILDKYDYSILVNSDDFLTEFVILIPKYY"
     gene            12863..13600
                     /locus_tag="MJE33_002650"
     CDS             12863..13600
                     /locus_tag="MJE33_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY9648579.1"
                     /translation="MIKVLICDDDKIVRDDIANIAKESGYVDYVKKVKDGIEAIEFIK
                     NEKIDLLIIDIDMPYKNGIDSAEEILSIDKDIHIVFVTGFADYSLESFKVHPIDFVVK
                     PFKKEKILDSINIAIDHINSHKIANNNFIEDTLFVYKIRKQIHMINFDDIVMFEKNSR
                     AINLHTKDEDIIKFYENFDDLKKRLPPNFFMTHRQYIVNLRFIHKVIPINKSSLEIRF
                     INFQENAILNKSLEKDFLYRFYRVKRF"
     gene            complement(13785..18842)
                     /locus_tag="MJE33_002651"
     CDS             complement(13785..18842)
                     /locus_tag="MJE33_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HBY9648580.1"
                     /translation="MLERTRVEKNPKIIENFKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALELIFTSEQVEELCQLIKR
                     KMDYTYSGGYYRKGYRSNKLVDYFRGIIRFISNYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQKVISALEEVIFGENQTALLTRQMIIGIMKSHSDKCYEMLSKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKPGIIRKCLELSYKSLTDEKFRIESINSNDNLEIYFALWATATYNIYDSYSLIENLL
                     SCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETNFDLSNTPIDKDFDARKEQFESLKHTLEILNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNRETVLSLLRKLELTDVELDKITNLLSLKTSSIRQTVIKILLEQDDSNLENI
                     ASSLIKSSNSQKCLGGLEIVNLLKDNTARQELYNKLILLIEDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNPENKVQIPEQFDFKDDSNSSSKLNKIISNISLSTREDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENADYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRETSKKLDLDYKLILDILFYSISTCGYNETEKSWF
                     SSIYSNVLDSKKQNKIYTKLEKLKFNGLTRKLLRVLLNEFDHKEIFNYCLEIYYKLRN
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTEEQVNREMISGYNASNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSNLANNIQYFEGINYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPRSFIDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACTIALENLA
                     RSAGFGDVNRMMWALETEKLNEVAHLFKPKDIDGTSIWIDVDENGKASTKVQKGNKSL
                     KSIPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIEGETYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQIVQPFKQVFREYYRINSDELSEKTISRRYAGHQIQPKKTVALLRNQLW
                     TVDYEEGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELASIP
                     PILFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKINNVNFEGSHA
                     LIAGNLGEYSVHMGSGIVHKKGMGALNILPIHSQSRGKIFLPFADEDPKTAEIMSKIL
                     LLAEDSKIKDPSILSNL"
     gene            complement(18867..19286)
                     /locus_tag="MJE33_002652"
     CDS             complement(18867..19286)
                     /locus_tag="MJE33_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648581.1"
                     /translation="MQIYFRVKAVGKRKPMLELTPFDVSDNINCLKEAISEIVSKNVL
                     EYNEKIPEKSIINFLTNEEIESQADIGKVGFGSIYNENKQSVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLDSSINLKSGDIFTFIRLTLLAGRMF"
BASE COUNT         6931 a         2780 c         2905 g         6878 t
ORIGIN      
        1 ttacaatttt ataactttgg tttacaataa tatagacaat aaatcctaaa aaacataata
       61 agaaaaaagt gcttaatatt ttagaaagac tggtttagtt tacaccagct aagccagtca
      121 aggaataatt taaagtgtaa tatataataa tgaaaaagaa catgctatta gaaatttcta
      181 atgacatgtt ctttttttgt agaaatcagc attattatat aaaaatttaa tactataaat
      241 tttaattata ttgtaactaa gcatgtattt taaacatagt atgatataga cccaaaatgg
      301 agggattata aatgagaaat attatacttt atttaaatga tgatactttt atatctaaaa
      361 aatatccaga taaaaacttt agtaatttag attattgctt aataggaagt aaatgttcaa
      421 atagttttgt aaaagaaaag ttgattactt tttttaaagt gagaatacca gatatattaa
      481 aagacaaaag tatattaaaa gcagagttat ttattcatat tgattcaaat aagaatcata
      541 tttttaaaga aaaagtagat attgaaatta aaagaataag tgaatattat aatttacgaa
      601 ctataacatg gaatgataga gtgtctatgg aaaatattag gggatattta ccagttggga
      661 taagtgatac atccaactat atttgtttaa atattacggg aactataaaa gcatgggcaa
      721 tgaataaata tcctaattat gggttagctt tatctttaaa ttacccttat cagattcttg
      781 aatttacatc tagtagaggt tgtaacaaac cgtatatact tgtaacattt gaagatagaa
      841 ttatagataa ttgttatcct aaatgtgagt gtcttccaat tagaattaca ggtccaatgg
      901 gaccaagagg agcgacagga agtacaggac caacgggagt aacaggccca accggaagta
      961 caggagcgac aggaagcata ggaccaacag gcccaaccgg aaatacagga gcaacaggaa
     1021 gtatagggcc aacgggagta acaggcccaa ccggaagtac aggagcgaca ggaagtatag
     1081 gaccaacagg agtaacaggt ccgacaggaa atacgggagt gacaggaagt ataggaccaa
     1141 cgggagcaac aggcccgaca ggaaatacgg gagtgacagg aagtatagga ccaacaggag
     1201 taacaggccc aacaggaaat acaggagaaa taggaccaac gggagcaaca ggtccaacag
     1261 gagtgacagg aagtatagga ccaacaggag caacaggacc aacaggagaa ataggaccaa
     1321 cgggagcaac aggagcgaca ggagtgacag gaagtatagg accaacagga gcaacaggtc
     1381 caacaggagc gacaggagtg acaggagaaa tagggccaac aggagaaata ggaccaacgg
     1441 gagcaacagg cccaacagga gtgacaggaa gtataggacc aacgggagcg acaggagaaa
     1501 taggaccaac aggagaaata ggaccaacag gagcaacagg cccaacagga gtgacaggaa
     1561 gtataggacc aacgggagca acaggcccaa caggagcgac aggagaaata ggaccaacgg
     1621 gagcaacagg tccaacagga aatacaggag taacaggaga aataggacca acgggagcaa
     1681 cgggtccgac aggaaataca ggagtgacag gagaaatagg accaacggga gcaacaggcc
     1741 caacaggagt gacaggagaa atagggccaa caggaaatac aggagcgaca ggaagtatag
     1801 ggccaacggg agtaacaggt ccaacaggag cgacaggaag tataggacca acgggagcaa
     1861 caggagcgac aggagtaaca ggcccaacag gtccaacagg agcaacaggc aattcctctc
     1921 agccagttgc taacttcctc gtaaatgcac catctccaca aacactaaat aatggagatg
     1981 ctataacagg ttggcaaaca ataataggaa atagttcaag tataacagta gatgcaaatg
     2041 gtacgtttac agtacaagaa aatggtgtgt attatatatc agtttcagta gcattacaac
     2101 caggttcatc aagtataaat caatattctt tcgctatcct attcccaatt ttaggaggaa
     2161 aagatttggc agggcttact actgagccag gaggcggagg agtactttct ggatattttg
     2221 ctggtttttt atttggtggg actactttta caataaataa tttttcatct acaacagtag
     2281 ggatacgaaa tgggcaatca gcaggaactg cggctacttt gacgatattt agaatagctg
     2341 atactgttat gacttaaaac gtgtctaaaa taatcttaaa aactatttag gctttattta
     2401 aatgacaaaa gtatttttat atattgagtt ttacctattt tagaatgaat aaaataacaa
     2461 taataataaa atatattcat aaaaatttta aatttatgga tttttattta actttattat
     2521 caatatatat atatataata aaaaactgtc tcaaatatag atttgagaca gttttcgtta
     2581 tttaaaaatt ttatattatt taaaattttt gattgcagta gttaaattag ggactaattg
     2641 tttttttctt gatacaacac ctggtgcaaa tgtaccttga acaccttcaa caccaaatgc
     2701 gttagcaatt aagctgtctt ctgctttata gattaaataa gaaccttctt tgattatatc
     2761 tgtaaccgca agaattagtt tgtcataatc agtcgaattt atataagata aaaactcatc
     2821 ttttttagca aatatagagt ctatgtctaa ggtaaatact tgtccaatac caactctatg
     2881 tccactcata ttaaattctt taaagtccat atttactatt tcttctatag tatattcatc
     2941 taaagaagta ccgcatttaa acatatccat agcgtatttt tccatgtcta cttttgctat
     3001 tttacttaat tcttcacaag ctttcttatc tatatcagtt gttgttggag acttaaataa
     3061 taatgtatct gataatatag cagataaaag aagcccggct atttcataag gtatctcaac
     3121 attattttct ttgtacattt gataaattat agtactattg catccaacag gcataactct
     3181 aaatgacata ggaacatcag tagaaatacc accaagttta tgatggtcaa ttatttcaac
     3241 tatgtttgct tgttcaattc catcagcact ttgagcatat tcgttatggt caactaaaac
     3301 aacattcttt ttagatgggt ttaatagatg accttttgaa actaaaccta aaaacttatt
     3361 atcatcatct aatattggat aagatctgaa gtttgtttct agcataatct cttttatttc
     3421 atccatataa tcatcttctt tgaatgtaat taaattttct gtagcttcta tagagtctaa
     3481 gaaactacat tgatttatta cattagaagt tttataattg tcactttcaa cagaaacaac
     3541 agacactcca tttatcttag caagagctag caaatctttt gaaatctctg tatgattagt
     3601 aagtattaaa agtttaactt ttgtatcaat agcatattca atactatcat atctatctcc
     3661 aacaatagct atgtctcctt cttttaaagt ctctattaga gtctccattc caaatgataa
     3721 agtgactact ctacctttta tatcttcacc acagtccaca agaatagtac cattaaggtt
     3781 tttacaaatg tttgatagtg atgtgtgtat agttgtaaag tgttgatgaa gtagattttt
     3841 agctatttct ttcatagtaa tgataccagc aaaagttcca tcatcataag ttacaggtag
     3901 tgttttaaca tttttttcat ccataaggtt ataagcctct aatatagatg aagttggtgt
     3961 taaaggtact actttatcat aatttaaatc tctaacttgt attctaacat tatctaaaat
     4021 ttcaggtgca tctaccttaa agtaatccaa ggcatattta gcttcttttc tgatatctcc
     4081 taaagcataa gctgttgctt ttattcctag tttgttcttt aaatgtgcta atgaaattgc
     4141 agaacatatt gaatctgtat ctggattttt atgtccgaaa actaataaac tcattttaat
     4201 actcctttca aaacttgaat ataataataa ttgataaaaa tattatatca aaaggtagtg
     4261 agtatttgtt gtaaaaagaa aaaaatttag tgaaaaaata ttatataaaa tttatttctt
     4321 acataaatac tacaaattta atataaaaat tattattata tatactttta cccaatcaaa
     4381 tatcgaatta aatttacttt tatttaaaat aaattgatta aatctacctg ggaaaaaatt
     4441 aattaatatg ttaataaatt aaaaatttat aaatatttta tactgtaaaa aattttgtat
     4501 atatattatc attaaaaaat taacggtctt aatataaaaa ttatttatct atatgaaaag
     4561 cataattatt acatattgac ttaaaaataa cttatagaga ttagaataat tttagtttat
     4621 atatttttta aaactataca gttgttataa aaagaaaaat tgcatacaag tcaaaaataa
     4681 gtgttatttt ataaaaatac gacaaaaatt ttatttacta cttatacaaa taacatgcaa
     4741 ataacatata aaatttaata agtaaaaaaa atgaaagata taaattatca ttttagtatt
     4801 ttatttagtc gaagtaaatt attcaaatga aatatatctc atttagataa aaaattttag
     4861 taaaagtaaa aaaaataata tatttgttaa aaaataaatt gacaaaatat tcagaaaaaa
     4921 acttcttgac ctaagagagt aaaataaata aaatctaagg tatagagatg aaatctataa
     4981 aattatttta agtataagtt aaaaatatag atgatgcatt taagatttat aaaaattaga
     5041 gatgcataat tattttaggg aggtttttag tatggagaaa aaagaaaagg tagtagaaaa
     5101 atcaaatgtt gaagtatgta gtcctgaaat tgtcaacagt gttgaaacat taagaatgag
     5161 attagaagaa attagattgg cacaaaaaga atttgcaaca tttacgcaag aacaggtaga
     5221 taagattttc ttagctgctt caactgcagc taatcaacaa agaatacctc tagcaaaaat
     5281 ggcagtggaa gagactggta tgggtattgt tgaagataag gttataaaaa accattttgc
     5341 ctcagaatat atctataatg cttataaaga tactaaaact tgtggtgtca tagaaaaaga
     5401 tgaagcattt ggttttacaa gaattgctga gccagtaggt gttttatcag cagttatacc
     5461 aacaactaat cctacatcaa ctgcaatatt caaatcccta atagcactaa aaactagaaa
     5521 tggtataatt ttctctcctc atccaagagc taaaaactgt actatagaag cagctagagt
     5581 tgtacatgat gcagcagtta aggcaggagc accaaaagga cttataggtt gggtagatgt
     5641 tccttcgatt gaattaacta atgttgtcat ggcagaggca gaccttatat tagctactgg
     5701 tggacctgga atggttaaat ctgcatactc ttcaggtaaa ccagctgtcg gagttgggcc
     5761 tggaaatgta cctgcaataa tagatgagag tgcagatata aaaatggctg taagttcaat
     5821 cttagtatca aaatcattcg ataatggtat gatatgtgct tccgagcaag ctgtaatagt
     5881 accagaaaaa atatatgaag aagttaaaaa agaatttaaa tatcgtggag cacatttcct
     5941 aaataaagaa gaaactgaaa aagtaggaaa agtagtaata attgatggtt cattaaatgc
     6001 cagaatagtt ggacaaccag cacatgtaat agccaaaatg gcggatgtag aagttccaaa
     6061 aacagctaga ataataattg gtgaagtaga atctgttgaa ttaaatgaac cttttgcaca
     6121 tgaaaaactt tctcctgtac ttgcaatgta caagtctaaa agttttgaag atgcagttgc
     6181 taaagcagaa aaattagttg cagatggagg ctatggtcat acatcttcat tatatgcaga
     6241 ctctataaat catccagaga gagtagaaaa gtttgtaaat gctatgaaga cttgtagagt
     6301 attagttaat actccatcat cacaaggtgg tataggtgat ttatataact ttaaattggc
     6361 accgtctctt actcttggtt gtggttcttg gggtggaaac tcagtatctg aaaacgttgg
     6421 agtaaaacat ttgcttaata tcaagacagt agctgaaagg agagagaata tgctttggtt
     6481 cagagcacca gaaaaggttt atttcaaaaa aggatgttta ggtgtagcag caagagaatt
     6541 taaggatgtt atggataaaa agaaagcatt tatagtaaca gattctttcc tatataataa
     6601 tggatatact aagaaattaa ctgatttatt agatgagatg ggaattaagc atactacatt
     6661 ctttgatgta gctccagacc caacattagc ttgtgcaaga gaaggagcta aagctatggc
     6721 tgatttccaa ccagatttaa taatagctgt aggtggagga agtgcaatgg atgctggtaa
     6781 gataatgtgg gtaatgtatg agcatccaga agtagatttc caagacttag ctatgagatt
     6841 tatggatata agaaaaagag tatatgtatt tcctaagatg ggtgaaaaag cgtattttgc
     6901 tgctatccca acttcagcag gaactggttc agaagtaact ccatttgcag ttataactga
     6961 ccaagattca ggtgtaaagt acccattagc agattatgaa ttaatgccaa acatggctat
     7021 aattgatgct gatatgatga tggagatgcc accaagatta acagcagcat caggtgttga
     7081 tgctttaaca catgcattag aagcatatgt atctatgtta agaactgaac cagctgacgg
     7141 tctagcacta caagcaggta aaataatatt tgaatattta cctcgtgcat ataaaaatgg
     7201 aaagaatgat aaagaagcaa gagaaaaaat ggcaatggca tctactatgg caggaatgtc
     7261 ttttgcaaat gccttcttag gaatatgtca ctctttagca cataaattag gtgcattcca
     7321 ccatgttcaa cacggtgttg caaatgcatt attaataaat gaagtaatca agttcaattg
     7381 tgcagaagct ccaaataaga tgggtgcatt ctcacaatac agatatcctg attgcataca
     7441 aagatatgct gaatttgctt cttttgcagg tattaaagga agtacagacc aagaaaaagt
     7501 agataatctt ataaaagcaa tagatgaact aaaggcaaaa gtaggtcttc ctaagactat
     7561 aaaagaggct ggagtagaag aatctaaatt cttagaaaga ttggatgcta tggttgaaca
     7621 agcttttgat gaccaatgta caggtgctaa tcctagatac ccattgatga gtgaattaaa
     7681 agagatatat ctaaaagttt attatggtaa ataatatcta ttgtgcaaca taggttctat
     7741 tgggtttgta tataaattat tagatttgaa tataaataga gttttattag agacacgtag
     7801 aagtaactat aaataataga ttataattta aattctaaat ggaaatgata aaaagtcagt
     7861 aatttaatgg taatttcgaa tatgtgtatt taatcaatta actttaaaat tagtcggaaa
     7921 atttttttat tacatataat tttaaagcat aatatataaa aaactttatg aaaaaattat
     7981 aaaaatattc agaaaattct attgactaat ttatttatca tgatataata agtttagaaa
     8041 agaaaaaaat tggggcctat taaggccccc ttactatttt ttgatagatt tatttaaaat
     8101 tactttctca ttttgatttt ttatatttat tatagagtca gtataatcca cttcaaagtt
     8161 ggcaaaagca gagaataaat ctatatctct taacaaatga attgtcatac attgattgtt
     8221 cttatttagc cttagaatca atgctcttac aaatggaaat tgtattctag aattaaataa
     8281 gttagaacca tcagtatcta tatctaagct acgataatta atatctataa atatatctac
     8341 atcttcattt tcttctctta ttattctgta gtcaaaatcc aagtttaaat ttaaattacc
     8401 taaaatcaat aacatttaaa tcctcttttc tataaatagt tttagagtgt taatttaagc
     8461 tctccatcta ttatagatat aattctatct gcttttctag caatattttc attatgtgtt
     8521 attattataa gtgtttggtt atattttttt atagaacttt ttagtaaatc catgacttct
     8581 tctgtagttt tactgtccaa atttcctgtt ggttcatctg ctaggattat acttggtttt
     8641 gcagataaag cacgtgcaat agcaactctt tgctgttgac caccagataa ttggttggga
     8701 agatgatttt tcctttcatt aagacccaat aaatttataa ggtcatcaat atattcttta
     8761 tctatttttt tattgtctaa ctttagtggt aattctatat tttcctttac atttaataca
     8821 ggaattaagt tatactgttg aaagacaaat cctacctttt gtcttctata tcttgctaat
     8881 tctttatctg aaagacaaca tatatcttta ccatctacct ttatagtacc attacttggt
     8941 ctttcaagac cacctactaa atgtagaaat gtacttttcc cacttccact tggtccaatt
     9001 atagcaacaa attcgccttt atttattttt aaatttgcat tctttagtgc atgtacagat
     9061 gtttcctttt ttccataaat cttacctaag ttaatagttt ccaatatagg cataattatt
     9121 cctcctattc tgtaatgttt atagcttcaa ctatactaat tttatttatc tttcttgatg
     9181 gaatatatgt agctagaata cctacaatta tatttgctac aacaactagt atatatattt
     9241 tgtaatctat tgaaaatccc aatccatatg atacaaagcc ctgcatataa tacattttaa
     9301 attgtattat aattcctgac acgatagtaa ttatacttga gagtacacca tataatattc
     9361 cttcataaag tatcatattt ttaaagcctc tctcagagat accaatagct cttaacattc
     9421 caaattcact agttcttgaa gttaagttat aacttatgtt gtttattatg ttaagtacac
     9481 tgattatgaa cattattgct actataccat aactataaat catagttcta gatgtcatct
     9541 tatcagaatt ttctttttct accatcatat ctactgttgt agttccagga acttcacttc
     9601 caattttacc taaaaggtta tttatttttt tcttgtttgc attttcatta aggtctgcat
     9661 aaactaagtt atacttatct acaccagtta atttttttag gtattcgcta ctcgttatta
     9721 catctacacc atcatctcca gagtacatac cattatctgc aaaaggataa cttataatag
     9781 ctccaacttt aaattcatat tctaagtatt cataattatc ttttagactt tcataaagac
     9841 ctgtatccat cccacccttt ggatatttga cttttatagt atcacctact tttatatcta
     9901 cagctggtgt tccttcacca ggagtaaaag tctttgtgta tttctgtgtt ttagaaattt
     9961 ctggtacata tacaatagct aaattttctt ttttcatttt tttcaagttt atatttccac
    10021 ttactaagta tttttctaat gactttatca tctcatcatt ataacctttt agcttttgct
    10081 ttagtgtata tttgccagta gtcttatttt ttatgaacag aggatttagc ttataataag
    10141 gagttgaatt tatagcttcg tagtaatcta atctatttat tttatttacc tctattcttg
    10201 tgtttaagat actagcagtt ttgactttgt caattccatc tatatttttt attttatcta
    10261 tctgattatc attaaaccct tttatatctt taaaagaagt attgttacca atttcattga
    10321 gataaacatt tgatagaata aaatctgcat tcatatatgt ttgttcttga gtagatttgt
    10381 tttgcacatc taaaaaagaa tatttataat tgtcttttat gacgattata cctcctatac
    10441 tcatagataa tacaattata atgaacccct ttttatttct aaagatgttt ttcgcagata
    10501 tagattttgt tactgacata tttaaagtta attttgatat taaataatta actttggtta
    10561 catttttatc agctccaaaa gtttttctga tagcatcaat aggagaaatt cttcttattt
    10621 ttaagtatgt aaagaaactt ataattagta ttattagtat tgtacatgca attgataaga
    10681 gaattatttt atctggaata acaaaaggtg taacatttat atttccttca aattgaatat
    10741 ttcctacaga cctattaaat atttgtgcac ctcccatacc tatacatata cctataggca
    10801 ttgctattag agcaagtatg aaaagttcat aaagcataag tttgaaaatc ctaaagttgg
    10861 tggaaccaat agctcttaag acgccatatt cctgtactct ttggtatatt gatataacat
    10921 agatactata tattacaata ccagcaaata gactcatagc aattgcaacg tatttacttg
    10981 aattatctaa agtaccgttt ttcatagtag attctataag cattgagttt cttcctatac
    11041 ttttctcatc taacatagca ttttttatta tattgtctat gtttgtattt atatcactgc
    11101 cttcattaaa ttcaatataa gttgtaaaat cattcagttc tgattcattg agattagaaa
    11161 aaatttcaca ttgtcccttt tttttctcta taaatctatc ttcaaggatt ccaacaactt
    11221 taaaagtttc tggattttct ttttgatata atttaaaagt aaggttttga tttatctctg
    11281 gttttaagcc catactattt aaaatccatt tctcgactac cacctcattg ctagcttttg
    11341 gaaatcgacc ttttataagt ttagaatgac tagtcaaata atttttattt gtatatagta
    11401 tgtttatagg taatttttca ttaacatctg tagacgcata gtaagatgtt atacccagat
    11461 ttttaatatc tttgctagat tttatggact ttaattgatt tttctttata tttttaaagt
    11521 atacatggtc agttccaagc tctcttttca atctatctac atctgcttgc tgagcacttc
    11581 ttgagagagt accaatacca acaacgagtg atgtagcaag aattatactc acaacaattg
    11641 ctaaagacct agctttataa ctttttatat accttagtac tattttcaaa ttatctttca
    11701 tatttaccac ttcctttctt aactaaatca attatatatt tatctaaaat atatagcaaa
    11761 gtaagtattt tcaaatttta agtagttaat tgtacaaatt catctgtatt tttacaagtt
    11821 taattaatat atatgtaaat tttaactttt tacataattt ttttaaaatg atataatccc
    11881 tatataaatt gtagagggga ggggaatcat ggataacaat tttgtatttc ctatagtaat
    11941 actaactttg tttatattta gcaatcttat aatgaataag tataaatttt acaaagaatt
    12001 agacatatta aaaagaggca tattaacatt ttttatatcg tgttttgcat tgattttatt
    12061 tatggagggc actaaatata tacaattaag gttaggatat agttttttac ttgaaggagt
    12121 attaaatatt atactttttt tggttttaat tattttaagt ttttatacag gctttaaagt
    12181 tataagattg ataaataata ataatgaaca ggaatataaa ttacaaaaac tagatagtaa
    12241 aataaatcat aaggataata taattatcaa attaagaagt caaaaacatg actatttgaa
    12301 acatttgcaa gtagtgtatt ctctattaaa tactgggcta gatgaagatg ccaaagaata
    12361 tataatgaat atatcaaata gctttaaatc aaaaaattca aggtatggaa aagtatctta
    12421 tttagatgct atagcttcat taaaatacga ggaatgtctt gaaaaggata tatcttttga
    12481 tgtctacatt caagatttta tggataattt agagattgaa cctaatgata tgagttcagt
    12541 actattaaac ataatagata atgcaataga gtctttgtct actataaaaa aagaaaagaa
    12601 gtatataaga ttacatgcat atgaagatga atttcagtat attatagcta taaaaaataa
    12661 tggtcctaaa atacataatg ttgaaagtat attttggaat ggatttagta ctaaagaagg
    12721 acaagatagg ggatatgggc tacatttagt taaaaatata ttagataagt atgattatag
    12781 tatacttgta aatagtgatg attttttaac tgaatttgta atcttaatcc caaaatatta
    12841 ttagttagga ggagaaaagt ctatgataaa agttttgatt tgtgatgatg ataagattgt
    12901 aagagatgat atagcaaata tagctaaaga gtctggatat gtagattatg ttaaaaaagt
    12961 taaggatggt atagaggcaa ttgaatttat aaaaaatgag aaaatagatt tattgattat
    13021 tgacattgat atgccatata aaaatgggat tgattctgct gaagaaattc tttctataga
    13081 taaggatata catatagtat ttgtgacggg tttcgctgat tatagtcttg aaagctttaa
    13141 agtgcatcct atagattttg tagttaaacc atttaaaaaa gaaaaaatac ttgattcaat
    13201 taacatagca atagaccata ttaactccca taagattgct aataataatt ttatagaaga
    13261 cacccttttt gtatataaaa taaggaagca gatacatatg attaattttg atgatatagt
    13321 tatgtttgag aaaaattcaa gagcaattaa tctacacact aaagatgaag atataataaa
    13381 gttttatgag aactttgatg atttaaaaaa acgcctacct cctaatttct ttatgaccca
    13441 tagacaatac atagtaaatc taagatttat acataaggtt atacctataa ataaatcttc
    13501 attagagata aggtttataa attttcaaga aaatgccata ttaaacaaat ctctagaaaa
    13561 agatttttta tatagatttt atagggttaa aagattctag tgaagataga agaaaaatac
    13621 tttttgtgtg atataataag taccgtaaag ataatgtata agagaagttt atataaaaat
    13681 tatttgtaat tatgatttaa tctatataaa ttacagtata gtatatattg gactttctaa
    13741 tttttttaag aaagtccaat atattttaga gatagttttt atacttataa attacttaag
    13801 atactagggt ctttaatctt actatcttca gcaagtaata gtatcttaga cataatctca
    13861 gcagttttag ggtcttcatc agcgaatgga aggaatatct tacctctact ttgagaatgt
    13921 ataggaagta tattcaaagc acccatccct tttttatgta caataccaga acccatatgc
    13981 acagaatact cacctaaatt acctgctata agagcatgag agccttcaaa attgacattg
    14041 ttaattttta ataataataa aagttcttta gcaattgcta tacgcatctc tacagtagaa
    14101 tggctagttt cagggtcaac accaccagca tgagcgacac ttacaacaag gtctatatca
    14161 cgcataactt ctgagaatag aattggaggt atagaagcta attctaaact tttataagtt
    14221 tttctatcaa aaaattctac tgtttcaata gtaggaggct ctatatctgc tggtgaaaac
    14281 caatctgcaa gtgcaaacat ctttgccact atattttctt tataaaatac tttctgtaat
    14341 ccctcttcat aatcgacagt ccaaagctga tttctaagaa gtgctacagt tttctttggc
    14401 tgaatttggt gaccagcata tctgcgagat atagtttttt cagataattc atcactgttt
    14461 attctataat actcacggaa tacttgctta aatggttgaa caatttgctt actaaataaa
    14521 tcatgttgga actcactcca cttaccagat tcaaacaaat gaactggatg tgcaagtttt
    14581 aatttatttt ttgccgtcaa cttataagtt tcaccttcta tattgcaaat aaagaactta
    14641 gacttatcat tataaacata tccaatatta tcatcacata cccatactag atttttaatc
    14701 aaagggcaga taactggatt tttatagata ttggcaattt ctttaactgt aaatactatt
    14761 tcatttttca tagcttgttc taaactctgt tttgcacgag aatactgact ctttaaatct
    14821 ttttggacag atttaagttc aagtacatat ggatgtttag caatagcttt tggtatactc
    14881 tttaaagact tgttgccttt ttgaacttta gtagatgctt ttccgttttc atcaacatct
    14941 atccaaattg aagtaccatc aatatctttt ggtttaaata gatgagcaac ttcatttagt
    15001 ttttcagtct caagagccca catcatacga tttacatctc caaatccagc acttctagct
    15061 agattttcta gcgctatagt acaagctttt gattcacttt cacgacgttg tgcaccaaat
    15121 tgcttgcttt ctttagcaaa cttttgaata aattcataac ggaaagttgc ttcttttagg
    15181 tctccttttt tcataggaag taaagagaaa cttctcaatt tatcttgatt tctatttgtt
    15241 tcaatttctt tcatcaacat gtctttatca agcttaccaa gtgtagcatc agcatagagt
    15301 tgtgcacgtc tatgagagtt accaccacta gttatatatt tagctgactt gtaaagtata
    15361 ttgaatcttt cttccccaat agttttatat gaatctaaaa accaatctac atcaaatgca
    15421 ccatcaatga aactacgtgg tgaaataggg gagaatagag caacctctga ttctttttgt
    15481 gcagagaagc cttcatttat atgtgcatgg aagtaccaaa ttgtactttt cagacccttc
    15541 cagtttaaat atttttcaat tatgtttacc cattgagggg catacatagc agattctatc
    15601 aatcttaact cagtaatgtc ggtattttta agtaaacttt ttaatttttt tgaatcatct
    15661 tctttagtag gatagcaatt tttaagtaga gaacttaaaa catctttttt tgaataattg
    15721 ctaccatatg aatatcctct ctggaaagtt tctttaccaa gagcgaccaa aatatttaca
    15781 aaataattta taccttcaaa gtattgtata ttattagcta gattagacac ttctgttggc
    15841 atatcacctc tacgtgattc aatttctata attcggtttg taacagtttt taaattatct
    15901 ttaagccaag ggtacttttc taatgtgtcg ccagcccatc taggatttgt caatgttctt
    15961 attatatttg atgcgttgta tccacttatc atttctctat taacttgctc ttcagtcatt
    16021 aatcctaatt catgtgcacg agcaaacaca tttatgctag tatagaaagg aaacttatat
    16081 tctagcaact gataatttga aaacgtagtg tagaaaaact tagtaaaaga aatatcatca
    16141 taaataaacg aatgtgcttt atcaagccaa tagtttaaaa taggggcatt taaagcaggg
    16201 cagtattcat tttgtctgta atagttttta gtaaattcaa ctctttgttt tttacattgt
    16261 tctatagtca ataagtttct aagtttatag tatatttcta aacaatagtt aaatatttct
    16321 ttatggtcaa actcatttag taaaactctt aaaagttttc ttgtaagtcc attaaattta
    16381 agtttttcaa gttttgtata tattttattt tgttttttag agtcaaggac atttgaatat
    16441 atagatgaaa accaactttt ttctgtttca ttatatccac atgtacttat actataaaat
    16501 aagatatcaa gtattaattt gtaatctaag tcaagttttt tactagtttc tctccaaagc
    16561 tctgctaatg gataattatc aagagtatca ttagaaactt tatatttaat aggagtcaag
    16621 ttataagtag aataacctaa aagtactttg tctacattac ctgaccatct ttcaacttca
    16681 tattcataat ctgcattttc agtaataagt tcatcaaatt tttcataaac ttttattatt
    16741 ttatttatat ccgtatcgag aatttttttt gcttcttttt ttgttgctac agaaacagaa
    16801 tcctcacgag tagataaact tatatttgag ataattttat taagctttga tgagctatta
    16861 gaatcatctt taaaatcaaa ttgttctgga atttgaactt tgttttcagg gttatatatt
    16921 ccaaaaccat tttctttgca gtaatcattt tttgctttac ctaacaaatt atcaattagt
    16981 atttgaactt gagatgtatt ctttttagat ttatcaatat cctctataag taaaataagc
    17041 ttattatata gttcttgtct agcagtattg tcttttaata agtttacaat ttcaagacct
    17101 ccaaggcatt tttgagaatt tgaagatttt atcagtgaag aagcaatatt ttccaaattt
    17161 gaatcatctt gttcaagtaa aatttttatt actgtttgtc taatagagct tgtctttaaa
    17221 gacaatagat tggttatctt atctaattct acatctgtta gctcaagctt tctaagtaga
    17281 ctcaataccg tttctctatt ggaaacactc ttatcactta aacattccaa taaaaattca
    17341 cgttgtctat ctgtctttgg ttcatttaca aaattgacta taagtgaagc tcttaaatct
    17401 gaactcataa gatgacaatt ttccataagc acatctataa attgagcatt catatcgtat
    17461 gctgttaaag tcatcataca tttaagtatt tcatgagaag aaatttcaat ccaagtccat
    17521 ggaaatacac ttcctgaaaa tgattttgtc tttgaattta aaatttctaa agtatgtttt
    17581 aaactttcaa attgttcttt tctagcatca aagtctttgt caataggagt gtttgacaag
    17641 tcaaaattag tttctctgtt ttgccaacta tagtagtagc caacagaacc ttctacatag
    17701 aaatttgtaa ttacccatgc tagtatctct aaatcatcac ccttaatgtt tttagatgct
    17761 atagaatgtt taaaatatgt actttctgta tgcgataaaa agtaaagagc tacaagtttt
    17821 ttatattttt cctgacaaga aagtaagttt tcaataagag agtaagagtc atatatgttg
    17881 taagttgcag tagcccataa ggcaaaataa atttcaagat tatcattgct attaattgat
    17941 tctatacgga acttttcatc agtcaatgat ttatatgaaa gttctaaaca tttacgtatt
    18001 attccaggtt tttggtctga aaaaccaagt cctgtccatg tatccaaggc tcttataaca
    18061 gaactaaatc gctctaaatt gttgtccaaa atagttttta acatgtatat tgatgcagaa
    18121 attgtaccct catctaaact ctctacaatt tgctgtctaa gtccttcttg ttgtttggct
    18181 gccaaaagaa gtttagaaag catttcatag catttatcac tatgactctt catgatacca
    18241 attatcattt gtcttgtaag tagagcagtt tgattttctc caaatattac ttcttctaaa
    18301 gcacttatta ctttttgatt tcctttgtct atttctagtg ctattttatc actcattata
    18361 gagtcatagt acaagacctt atttggaaga gtaagataat ctataactga aaaatcataa
    18421 taaattaaat caacatagtt tgaaataaac cttataatac ctctaaaata atccacaagt
    18481 ttatttgagc gatacccttt acgataatat cctcctgaat atgtataatc catctttctt
    18541 ttaattaatt ggcaaagttc ttctacttgt tcagaagtaa atattaattc taatgcattt
    18601 tttaaaggtc cagaaaggag ctcgtccata tttttaattt cgtagtagtg gcaattgtag
    18661 tatttcttta tcatatttaa gaatttgtct tcaggttctc tataatagta tgaactattt
    18721 tctatgcagt cccaaagaga ctttgctaag tcgtagtact gttttgaaga agatttagta
    18781 aaattatttt taaaattttc aattatttta ggattttttt ctactctagt acgttctaac
    18841 atttatgtac ctccaaatta tttattttaa aacattcttc cagcaagcaa ggtaagtctt
    18901 ataaaagtaa atatatctcc acttttcaga tttatagagg aatcgagttt ttcaataaca
    18961 tcctcattta taagcacttt gtagattcca tcttcaaatg cctgtaacgc tacctctatt
    19021 gccttttcta cagattgttt attctcattg taaatagagc caaatccaac ttttccaata
    19081 tcagcttgag attcaatttc ttcattagtt aagaagttta ttattgattt ttctgggatt
    19141 ttctcattat attcaagaac atttttagat acaatttcag agatagcttc ttttaggcaa
    19201 ttgatgttgt cagatacatc aaaaggggta agttctagca taggtttgcg tttgccaaca
    19261 gccttaactc taaaataaat ttgcataaaa tcacctcgca aaatattaat tatgtattta
    19321 gaaatttaaa aatttatcta tttgttatat tatatcattt tgtgaattat taatgtgaat
    19381 tatggaatta ttttacaaaa aatagaaaaa aagctatctt aaagtctaaa aaagcaataa
    19441 aagataactt ttaactataa ttatatagaa ttataactgt acatttatta aatt
//
LOCUS       DAGSRM010000072        19238 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.072, whole genome shotgun sequence.
ACCESSION   DAGSRM010000072 DAGSRM010000000
VERSION     DAGSRM010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19238
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.072"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            336..581
                     /locus_tag="MJE33_002653"
     CDS             336..581
                     /locus_tag="MJE33_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648582.1"
                     /translation="MFIDEELEGYILTCKISEDFKNIPEYSDEEFYVTVYKDESSDSG
                     YYALLENKEERVVWDGEVVANNIFNNLWIVINKVKTG"
     gene            680..943
                     /locus_tag="MJE33_002654"
     CDS             680..943
                     /locus_tag="MJE33_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HBY9648583.1"
                     /translation="MEYSYSKMNLKKGDIVEVNLEKQANVILLDHINYVKFKNQRNYD
                     YYGGFAKKNPCRMRVPNTGTWYLVVNQDGNSGIVNFSINTIQN"
     gene            complement(1228..2175)
                     /locus_tag="MJE33_002655"
     CDS             complement(1228..2175)
                     /locus_tag="MJE33_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HBY9648584.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTVELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLADTQALAAEIHEEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEVCPVKAAKLTDKGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQIDRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGADKFIEMLLSDEVLERKEEILEALLHLTGGAVC"
     gene            complement(2178..3809)
                     /locus_tag="MJE33_002656"
     CDS             complement(2178..3809)
                     /locus_tag="MJE33_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBY9648585.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKIIEDKSSLIVNTPESFSKLTGAEVRCGVEVTSIDRNAKQVTLKTLSTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFMRTPNDAIKLRDSIENGIKRAVVIGGGF
                     IGLEIAENLSAMGIRVSVVDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIE
                     GENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNNTIIADEYMLTNDKD
                     IYVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPE
                     LNAGKTGLSKEIAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQV
                     MGPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGE
                     LDSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGK
                     NSYMAQNRLRRFGYTNTKVLEGGILFYPNLKVMEE"
     gene            3939..4856
                     /locus_tag="MJE33_002657"
     CDS             3939..4856
                     /locus_tag="MJE33_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBY9648586.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     VISPIMLELYKVNPLIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIKVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVEYGKKQARKKSRSVNSL"
     gene            complement(4913..5494)
                     /locus_tag="MJE33_002658"
     CDS             complement(4913..5494)
                     /locus_tag="MJE33_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBY9648587.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTDGKVTNDGDFIFYNNLKHASGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(5534..6100)
                     /locus_tag="MJE33_002659"
     CDS             complement(5534..6100)
                     /locus_tag="MJE33_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBY9648588.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLVVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNVRGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLEDIPEYV
                     HKIVFGVSIYKAKKRDQDFGQMNNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
     gene            complement(6556..7416)
                     /locus_tag="MJE33_002660"
     CDS             complement(6556..7416)
                     /locus_tag="MJE33_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBY9648589.1"
                     /translation="MLLKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGITGL
                     GVVLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMAT
                     LDITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGIV
                     DCVVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISKK
                     IMEDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPNAFIIITDIHE
                     ALGNGFKKIE"
     gene            7565..8455
                     /locus_tag="MJE33_002661"
     CDS             7565..8455
                     /locus_tag="MJE33_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBY9648590.1"
                     /translation="MAKLTLEHIKGNTYYIPLPTIVGVYVDGKDAILIDSGNNKDTAR
                     QVLRLLEEHNLNPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPSNGKIVDTELEAISLPGHYFEMIGVRTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKSLI
                     NINKKKMEEIIDNIKKVCYEPVMIDEVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDGGKMMIKAA"
     gene            8673..10427
                     /locus_tag="MJE33_002662"
     CDS             8673..10427
                     /locus_tag="MJE33_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp23"
                     /protein_id="HBY9648591.1"
                     /translation="MYIMNKKFKKFSKKLSILILVLTMFLFNNLTANAQIKEDSIIGI
                     DRYETAGLIADRQTYDTVILVNGDKNLSDGLSSSGLAGAINAPILLTKKSEIPKATLN
                     RLDNKSLNVVKKVYIIGGYNTIENSVEKDIKRKGIQVERINGNNRIETSYNVAKKINE
                     VSSVKEVMLTNGFVGEADAMSIAPVVAKNKGAIILTDGKSVPFKTEGLNVYAIGGKSA
                     INEDLVNETKAIRIGGNDRFETNKKVMEKFYNGATDFYITNGYQLVDALTLSPLAKEK
                     PIVLVADGSNKGILKGAKSITKVGGIDANIYKQCLDMIKYNNMDMTPNILKHLTSVEG
                     NEVSEILIESDNLGVVVEKTNTNKFEFDYVSVTNEKNCTFSVYKENSSNNIKNGKLVV
                     SAKKKIDKKDSDLQDMNGDNMINANKDRMVNVIKIGVPDKMYSNFKVDVESGSVELYN
                     IKGGATVDVNDGIANIVDNNVTNPFNINTNSGISGVTAETISSEIKFRANNGIVNVTA
                     TNINGNISLFGSDNKDTFDGIFKLNLKKEPSNLHLKLIGNGINKLPEGWSKDYILGNG
                     QPIIEVKNNGINNISIGE"
     gene            10811..11797
                     /locus_tag="MJE33_002663"
     CDS             10811..11797
                     /locus_tag="MJE33_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBY9648592.1"
                     /translation="MKKITINDIANLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIITPCLDSFVKSKIMMAIDEELKKLKYTSLIINTNRKI
                     RSEIDSISKLASLKVDGIILIGTEITKEHKNEIEKLDIPIVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIANKGYKNIVYLGVDESDISVGLNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     EMSIQRSGEMLESENPDIIICATDNIAIATMKEINKRGKSIPQDISVAGFGGYDVLSI
                     ISPKLTTIKFENKNAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
     gene            11966..12114
                     /locus_tag="MJE33_002664"
                     /pseudo
     CDS             11966..12114
                     /locus_tag="MJE33_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423822.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
     gene            12133..13614
                     /locus_tag="MJE33_002665"
     CDS             12133..13614
                     /locus_tag="MJE33_002665"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBY9648593.1"
                     /translation="MYKEPKYRTILESTKEELNKLRNISLNDKYKPLFHIHPQHGLLN
                     DPNGLAYYNGKYHVFYQWYPYDATHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     AYSGNAIEVDGKLHMYYTGNIKYSAEDRYAYQNLAIMNKDGKITKYENNPIVSEIPKG
                     YTGHVRDPKVFKRKDKYFMLLGAQTSDKKGVIIVYESKNSIDWNFKGELNVKNIDEDF
                     GYMWECPDYINIDEKDILIFSPQGVEPKGFDYQNIYNVVYAIGNMDLDNLTFEIDTMK
                     ELEKGFDFYAPQTFIKDSQIILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELIKLRYDETSGQNTIKNNINIIENDENVYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKLGNIVTLDRSNFKKVFGVEYGTNRKEYINIDENTNIKVLADRSILEIFI
                     NEGEVVFTSRIFAKENSNQIRVYSDKIVEYEYTKFKLKQGIEL"
     gene            13640..14611
                     /locus_tag="MJE33_002666"
     CDS             13640..14611
                     /locus_tag="MJE33_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HBY9648594.1"
                     /translation="MKKVISIGEALIDFIPNQNGGKLKNVSEFRRVAGGAPANVSAVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEVNVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNNCHILHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSEQSCKKAISEFLPFAHIVKISDEELEFITGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGVKVNAIDTTGAGDSYIGSFLYTLLYKQITLN
                     DIKEMKQETLNEYLEFSNYYAARSTTIKGAISSYATKEEITEFIKNL"
     gene            complement(14867..15469)
                     /locus_tag="MJE33_002667"
     CDS             complement(14867..15469)
                     /locus_tag="MJE33_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBY9648595.1"
                     /translation="MEVCILMGSPKKKGNTAAILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKILMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPALWAGKSVALITTCGYRPEKGADIWENGMKRYCKHSQLKYIGM
                     LVERDLGKPNFMNKEKEEHSRQFAKQIYYILSNSIELDNC"
     gene            15750..16961
                     /locus_tag="MJE33_002668"
     CDS             15750..16961
                     /locus_tag="MJE33_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HBY9648596.1"
                     /translation="MYSNEDYKFIEENAPCYIYDETNIIEKCKELKNNILGFDFLYSI
                     KTNPYNKIVKSIAEQGFGADASSTNEVMISSKCGVSKDDIYYSSPGKTEIDIETVLDK
                     CIIIADSITEIELINKISKKHNQKVKIGIRINPDFTMDDDSAKSSKFGIDIEDMSSVF
                     KMLSMCDYVSVVGIHIHIKSQVLDFEKLGHYYEKCFFIALNLNKSKYINIEFINFGSG
                     LGVAYDNLRENPLNLKELSKLTQKIAFTNSNTLKSRLLIESGRFIVCKSGNYYVKIVD
                     IKVSRGKKYLIVKNGLNGFMRPIISNLIENITCGVELEGQEPLYTSNHAFSLSVLNNL
                     KEEEYVNVVGNLCTSLDVIANNVKLKKANIGDLISISNAGSYSYSLSPLLFSSYEFPK
                     QYYKRKDGYIE"
     gene            17029..17226
                     /locus_tag="MJE33_002669"
     CDS             17029..17226
                     /locus_tag="MJE33_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HBY9648597.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILPLYIKQLGVTDVSLI
                     QQYSGIIFGCTFVVAAFFSPI"
     gene            17260..18231
                     /locus_tag="MJE33_002670"
     CDS             17260..18231
                     /locus_tag="MJE33_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY9648598.1"
                     /translation="MLLRASIGMGIVIFTMAFVQNVYQLLGLRILQGVFTGYATACTT
                     LIATQTDKNHSGWALGTLATASTTGSLIGPTVGGYIESILGLKSTFIITGGLLFVSFI
                     ISILFVVDNFKPKEIKESISIPKEQLNILPNKFLIASIFVTTFITQLALYSIEPIVTI
                     YISQLTNFASNVALIAGLTFSASGLANLLAAQKLGKMSDKIGPQKVLLISLLWAGIIF
                     IPQAFVKTAWQLMLLRFLLGLSVAGLNPSVNSLLKKIAPEEYVGKIFGYNASAQYIGC
                     SSGAFLGGQISAHLGIRTVFFSTSLLLFRNALWMYKFTGLFIKDKTK"
     gene            18418..18795
                     /locus_tag="MJE33_002671"
     CDS             18418..18795
                     /locus_tag="MJE33_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBY9648599.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMERKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFESHFKNLEEE"
BASE COUNT         7457 a         2333 c         2878 g         6570 t
ORIGIN      
        1 aattttatta tattttaatt gaaataatct actttgaaaa ttactaactg acaaaagaat
       61 atgattatag tatagaaaaa atgtaaatta tttaatatta agtgagagta tatgcttacc
      121 tatataaaat cgtataaatt aatggctaaa tttataaatt ataaagaaat atataggaaa
      181 ataaaagaat taattagtat aatataaagt taaaaagtga aaatttatat aatttattta
      241 taaagtaaag aaaaaataga aatttgtaat aaaataaaaa gtttatgaca taatatggta
      301 taattagtaa aaatatagag attggaggaa ttgctttgtt tatagatgaa gaattagaag
      361 gttatatatt aacatgtaaa atatctgaag actttaaaaa tatacctgaa tatagtgatg
      421 aagagtttta tgttacagtc tataaagatg aaagttctga ctctgggtac tatgctttat
      481 tagaaaataa agaagaaaga gttgtatggg atggagaagt tgttgccaat aatattttta
      541 ataacctttg gattgtaata aataaggtta aaactggata atagtctaat tatatagtat
      601 ttgtaatatt ttaaatgaat ttttacatat ttgagatgag taaataacaa agattattcg
      661 atactggagg agtaattcaa tggaatatag ttatagtaag atgaacttaa aaaaagggga
      721 tattgtagag gtaaatttag agaaacaagc caatgtaata ttgttagacc atataaatta
      781 tgtgaaattt aaaaatcaaa gaaattatga ttactatggt ggatttgcaa agaaaaatcc
      841 atgtagaatg agagttccta atactggaac atggtatcta gtagtaaatc aagatggaaa
      901 tagtggaata gtaaactttt caatcaatac aattcaaaat taattttaga atattggata
      961 ttcttacttt ttgtactttt tatatagtta aaataaaatt aataagcaat tattataaaa
     1021 taatgtgggt gcctcaaaat gaattttata attcacgaga cactcttttt tatatgaact
     1081 caaatatact ttatcatttc aacaatgaaa aagaggcgta tctgtgtttt gagatagcct
     1141 catttatcat atatcatata tagaatttta attattctta actaaaattt tttccatagg
     1201 ttatctgtac aattaaaata tttattgtta gcatacagct ccacctgtaa ggtgtaaaag
     1261 agcctctagt atctcttctt ttctttccaa aacttcatct gataaaagca tttctataaa
     1321 tttatcagca ccgattctat cgatagttat tccaaaacgt tcacctgtgt taccctgttc
     1381 tctaaataaa agaattgctt tttcaattaa agtcataagt tcctcttttg aaaataatct
     1441 atcaatctgt gttccaggac gaactgattt tccccatttc cctcctatat agactttata
     1501 accagattct ttttcttcaa tactatcaaa attacaactt tctatacact taccacagtt
     1561 attacataga ttactatcta tttcaagttt acctttatct gttaatttag ctgctttaac
     1621 aggacagacc tctacaacag cacatttttt acaaccaaca caaagctctg aatcatagtc
     1681 aggtacacgt tgaccaacaa ttccaagatc gtttaggtca ggtttaatac agttattagg
     1741 gcatccacca acaccaattt tgaacttatg aggtagtttt acatcatacc aatttttgta
     1801 aaattcttcg tgtatttcag cagcaagtgc ctgtgtatca gctagaccat gaacacaaac
     1861 agtaccttta caagccacaa caggacgaac ccttgatcca gttccacctg ttattagatt
     1921 ttcgctagca ataaattcac ttagagcttc aatatcttca aattttacac caggtaattc
     1981 tactgttagt ctagtagtaa atgctaactg accgtttccg aatttttcag cagcttctgc
     2041 aatttttttt gcttgtgatg catttattac accatttaca gttattatac gtgctgcaaa
     2101 atgttctcca tccctgcttg gtagaaaacc tctacctttt acttcctttt tttgttctgc
     2161 tgtaacatta ctcatattta ctcctccatt accttaagat ttggataaaa tagtattcca
     2221 ccttcaagaa ctttcgtatt tgtatagcca aagcgtctta agcgattttg cgccatatag
     2281 ctatttttac ctttagcaca tataagaaga attttttcat ccttagcaag gccttcaatt
     2341 tctccattaa tttcaccaac aggaatataa cgtaaactag gaatagaagg agcttttgaa
     2401 acatcaagtt tagtccaact gtcaaaatcc tcaatttcgt caagtaaaac actatcaagt
     2461 tctccattaa gtttattttg taaaacattt actgccacta caaaagggtg aatagctgtt
     2521 gaaaaaggtg gtgcatatgc aaggtccata ctttctaatg aataaaggtc agcgcctaaa
     2581 gttattgcag tagcagcgat atctactatt ttatcaaccg ctccaggtcc cataacttga
     2641 acacctaata attttctagt atttttttca gcaaccattc ttataataaa attagatgca
     2701 cctggataat aatgagcttt atcatcagta gctatggtta cagagcaaac attataacct
     2761 tctttttcag ctatttcttt acttagacct gtttttcctg cattgagctc aggtaatttt
     2821 actactgttg ttccaagaac accattgtaa gttttagatt ttccagatat attttgtgcg
     2881 caaattctac cctcatgatt tgcagatgac cccataggtg accatgtagg tttatttgtt
     2941 aaagaatttt ttacaaaagc acagtctcca actacgtata tatctttgtc atttgtaagc
     3001 atatattcat ctgcaataat tgtattatta ggggcaaatt ctaaacctgt gtcttttaga
     3061 aaacctgtgt taggacggat tccaactgac ataaccacta aatctgtttt aatagctcta
     3121 tttttagtac gaagtttttc aactttattt tcaccttcga tacctacaac ctggtcacct
     3181 gtaaaaatca ttatgccttt atcagccata tgattttcaa cgtattcagc aaaatcagta
     3241 tcaaatccag gcataacatg ttcagccata tcaacaacac ttacacgtat acccattgca
     3301 gaaagatttt cagcaatttc aagaccaatg aaacctccac caataacaac agctctctta
     3361 ataccgtttt caatagaatc tcttagtttt atagcatcat ttggagtacg cataaagaat
     3421 actccaggta aatctattcc aggtattggt ggttttattg ggtctgcccc tgtagcaatt
     3481 acaagtttgt catagcttag attttctttt tctcctgttg aaagcgtttt caaagttact
     3541 tgttttgcat ttctatcaat agatgtaact tcaactccac aacgaacttc tgctcctgtt
     3601 agtttagaaa aactttcagg tgtatttacg attaaagatg atttatcttc aattatctta
     3661 ccaacgtaat atggaagacc acaacctgca taagaaatat catcaccttt atttagtatt
     3721 gtaacagagc aattatcacc catttcacgt tttatttttg cagcagtttt tgttccagca
     3781 gcaactccac ctataattaa tactttcaca atatatcaca ctccaatcta ctttataact
     3841 ctataataaa attatacttc ttgacaatat atttgtaaaa tactaaaata ttaataaaat
     3901 cataagcatt tactaatgtt tgctaaggtt gtgataatat gacaattcgt agtcttgaaa
     3961 tctttgttaa ggttgctgaa tgtggtaaaa tgagtgaagt agcaagaaat atgtatatta
     4021 cccagtcatc agttagccaa gctatttcag aaatagaaaa agagtatggt gttaagctat
     4081 ttgatagaat ttcaaaaaaa ttatatttaa ctgaggcagg gaaaaaactt cttggatatg
     4141 ggaggcatct tttagctgta aatgaagaaa tgaatgattg tatgaagcat tgtgcaaaaa
     4201 atatccgtat aagagttggg gcaacagtaa ctgttggtac atgtgtaata agtccaataa
     4261 tgttagaatt atacaaggta aatcctctta tagaacctga agtatttgta gaagatacaa
     4321 gactgattgc aaaaaaatta cttaatagtg aattagatat tgcgattgta gaaggaaaaa
     4381 ttaagcaccc agatattgtt acaaaaagca ttattaatga taatcttgta cttatatgtt
     4441 cacacaagca tgagttttat aaaagagact ctataaaagt ttcagaactt tcaaatcaac
     4501 cattaattat gcgtgaatta ggaagtggta caagagcaca attagaaaag caattaaaag
     4561 aattaaaaat cccaatgaat attagatggt catgttataa ctctgaagct atattaagag
     4621 cagttgtaga taattttggc attgcagtta tatccgaatt acttattgag gattatttaa
     4681 aaaagcatct tttgtgggct tgtgatattg aaggtattaa tttacatagg acatttgata
     4741 ttgcatatca tagaagcaag ttttttacag aaaatatttc agcatttttt gatatttcag
     4801 tagaatatgg gaaaaaacaa gcgagaaaga agagtagaag tgtaaacagt ttataatata
     4861 tctaaataaa aataagagta tatcaatata ctcttatttt tatttagata tattagccta
     4921 tattaacgcc aaaattttta cacaatcctg caagtccatc ctcaaatcca gaacctaaag
     4981 cattaaattt ccattctcca ttatgtctat atagttctgc cacaactatt gctgtttcta
     5041 tgctaaaatc ttcacccaat tcatatttaa taagttcctc attagtttct tcattatata
     5101 ttcttatata tgaattttca acttgtccga aattttgtct tcttgtaatt gcttcgttta
     5161 tagtaacact aaatgatatt ttagcaatat tttgaggtat tttagataag tctacattta
     5221 tttgttcatc atcaccatca ccaacacctg ttctattatc acccatatat tcaacagctc
     5281 cagaagcgtg ttttaaatta ttatagaata taaagtctcc atcattcgtt acttttccat
     5341 cagttccagt taaaaatgca gaggcatcta aatcgaaatc aaagccacca tcatatttat
     5401 taatatccca tcctagtcct actattactt tttttagacc agggttactt tttgttaaac
     5461 ttactttttg tccttttgct aaagttatac ccatttaaac caactcctca ttataaattt
     5521 tttattgtaa ttattactta tatgtattaa ttaattcacg aagctcttca ttttgaccaa
     5581 ttcctaaagc attaaatttc cattcttcat ttctacgata aatttctcct gccaaaactg
     5641 ttactttacc agaaaagtct tcttgaagat tatatttaaa taattcttgc ttggaatttg
     5701 aatcaattaa tcttataaaa gagttgttca tttgaccaaa atcctggtct ctttttttag
     5761 ctttgtatat agaaactcca aaaactattt tatgtacata ttcaggtatg tcttctaaat
     5821 taacaattat ttgttcatca tcaccttcac cttttccagt taagttatct ccagtgtgta
     5881 taatagcttt agaagggtgt ttaagatttc caaaataaac caagtcacct ctaacattca
     5941 ttttatcttg gttagtcaat gctattgcaa atgcatctag gtcataattt tttttaccca
     6001 atttattcat atcccatcct aagcctacta ctagattttt tacaggatta ttatctttta
     6061 ctaagctaac tctttgtcct ttttgtaaac taacactcat tttaagttct cctttttata
     6121 tttttagttt tattgatggt atcaagtata agaaccatat tagctatatt atatacgtat
     6181 ttaggctaag ttggctatat ttataaatta tttattatat aactacatga atattttata
     6241 atttaaacat attgacatta tatgctttaa attattttgt agttttataa tatttaaatt
     6301 tgtatttgat tgttataaag taaagtaagt aataaagtta gactcaataa ttctaaaatt
     6361 tctaagcata ctatctaaat gagaacggat ttggaaatac ttgtataaaa caataattga
     6421 tacatttaat ttttatctta aaaacaacaa ttataatata taaatagcaa gtataatata
     6481 taaatagcaa gtataatata acatataaaa agttgaattt gtatatgtta attatacttg
     6541 ctatttattt gttagttatt ctattttttt aaatccatta cctaaagctt catgtatatc
     6601 tgtaattata ataaaagcat tagggtcgac attttttact aattttttca attcgacaac
     6661 ttctttcttt tcaactacta ctaaaagtac atttttattt tctccagtat atgcaccttc
     6721 tccttttaga atagttgcac ctctatctaa atcttccatt atttttttac ttatatcttt
     6781 tgaaaggtca gatataatag tgaatgattt ggaataatta aatccttcaa taagcaagtc
     6841 actaactttt actaaaatgt ataaagccac agttgaataa agtgcaattt caacctgtct
     6901 atttactata cctgataaaa caactacaac gcagtctaca atacccatta aaacaggtat
     6961 actaaatgta ggaaaatatt tatttagtag gagtgccatt aaatcagttc cacctgtaga
     7021 gccatttatt ctaaaaataa gtccttgacc aactcccatt aaaatagaac cagatataat
     7081 tgcaagatac atatcatttg ttatatctaa tgtagccata tttgcagtaa gcttaagtgc
     7141 tatggtaaga aaaattattc ctaaaaccgt tttaaaacaa tctttttttg aaagtatttt
     7201 gtaagcaaat atgaataatg gaacatttaa aagtaaattg acaatccaaa gttctacacc
     7261 gaataatgaa tttaaaacaa ctccaagtcc tgttatacca ccagcagcga ttgcatgtgg
     7321 attaaaaaat aagttaagag aaatggccat taatatacag cctacaatta atattaaaat
     7381 ttctgaaaaa gttattctat cttttttcaa taacatcact ccttatatat agattataat
     7441 atatattata tagtttttcc aatttttaat aaaagaaata aaaatatatt ataaaaacaa
     7501 tttgattgga attttcagaa aattaatgct ataattaaaa gtaaatttaa agggaggtta
     7561 taaaatggct aaattaactc tagaacatat aaaaggaaat acatattata ttccactccc
     7621 tacaatagta ggtgtatacg tagatggtaa ggatgcaatt ttaattgaca gtggaaataa
     7681 taaggataca gctagacaag ttttaagatt attagaagaa cataatttaa atcctaaatt
     7741 aataataaac actcattcaa atgcagacca tataggagga aatgcatatt tgaagaatca
     7801 aacaaaatgc aagattgcta cgactaaaat tgaaggatat tttacagaaa atccaatatt
     7861 agaatcagca tttttatatg gtggctatcc aagtaaagca ctgaagaata aatttttatt
     7921 agcaaaggaa agtgaagttg attatattat accttcaaat ggcaaaattg tagatacaga
     7981 acttgaagcg atttctcttc caggtcatta ttttgaaatg attggtgtta gaactccaga
     8041 taatgttttg tttgttgcag atagtcttac accagagaat ataattacta aatatcattt
     8101 tttcttccta ctggatatag attcacagtt taagacacta gaaaaattga gaatgttaga
     8161 ggctgatttc tttgttccaa gtcactctgt caaaactaca gatataaaaa gtttaattaa
     8221 tattaataag aagaagatgg aagaaataat agataatatt aaaaaggttt gttatgagcc
     8281 agttatgatt gatgaagtta ttgaaaaaat gtgtgatttg tacaatgtca agcttgatgc
     8341 taatcaatat gtgttggttg gaagtacaat acgttcttat ataacttatt tatatgagaa
     8401 taatatggtt gaatatatat ttgatggtgg taagatgatg ataaaagctg cgtaaatagt
     8461 aataattttc aaattatatg atgaaattgt tttagtatga ctgttatttg aaaagtaata
     8521 taaaaaaatt tatataaata agctaagatg attgatataa agttactgat aattttaagg
     8581 taatatatac gttactataa tattatgtta ttatttgtaa ataaggggat aatttataag
     8641 caaattaaag accttaattt acaggaggaa tagtgtatat tatgaataaa aaattcaaaa
     8701 aattcagtaa aaaactaagt atattaattt tagttttgac tatgtttctt tttaataatt
     8761 taactgcaaa tgcacagatt aaagaagatt caataatagg aattgataga tatgaaacag
     8821 caggacttat agcggataga caaacttatg atactgtaat attagttaat ggagataaaa
     8881 atttatctga tgggctttca tcaagtggat tagctggagc tattaacgca ccaatacttt
     8941 tgactaaaaa aagtgaaata ccaaaagcta ctttaaatag actagataat aagagtttaa
     9001 atgttgtaaa aaaagtatat ataattggtg gttataatac tattgaaaat tcagtagaaa
     9061 aagatataaa aagaaaaggt attcaagtgg aaagaataaa tggtaacaat agaattgaaa
     9121 ctagttataa tgtagctaaa aaaataaatg aagtaagtag tgtaaaagag gtaatgctta
     9181 caaatggatt tgtaggagag gctgatgcta tgagtatagc acctgtagtt gcaaaaaata
     9241 aaggagctat aatacttaca gatgggaaat cagttccatt taagactgaa ggtttaaatg
     9301 tttacgctat aggtggcaaa tctgcgataa atgaggattt agtaaatgaa actaaagcaa
     9361 ttagaatcgg aggaaatgat agatttgaga ccaataaaaa ggtaatggaa aagttttata
     9421 atggagctac agatttttat ataacaaatg gatatcaatt agtagatgca ctaacattat
     9481 ctcctttagc caaagaaaaa ccaatcgttt tagttgctga tggaagtaat aaaggtattt
     9541 taaaaggagc taaaagtata acaaaagttg gaggtattga tgccaatata tataaacagt
     9601 gtttagatat gattaaatat aataatatgg atatgactcc aaatatttta aaacacttaa
     9661 cttcagttga aggaaatgaa gtttctgaaa tacttataga atcagataat ttaggtgtgg
     9721 ttgttgaaaa gacgaatacc aataaatttg agtttgatta cgtaagtgta actaatgaaa
     9781 aaaattgtac tttttctgta tataaagaaa attcttctaa taatataaaa aatggaaagc
     9841 tagttgtaag tgctaagaaa aaaatagata agaaagatag tgatttgcaa gatatgaatg
     9901 gcgacaatat gataaatgca aataaagata ggatggtaaa tgtgataaaa attggagttc
     9961 ccgataaaat gtattctaat tttaaagtag atgttgaaag tggttcagta gaactttata
    10021 atattaaagg aggagctact gttgatgtca atgacggaat tgctaacatt gttgataaca
    10081 atgtaacaaa tcctttcaat ataaatacta atagtgggat ttcaggtgta acagctgaaa
    10141 ctatatcatc tgaaataaaa tttagagcaa acaatggaat tgtaaatgta acagctacaa
    10201 atataaatgg caatataagt ttatttggaa gcgataataa agatactttt gatggaatat
    10261 ttaaattaaa tctgaaaaaa gaaccatcaa atttacactt gaagttaatt ggaaatggga
    10321 taaataaatt gccagaaggt tggtctaaag attatatttt aggaaatgga caaccaatta
    10381 ttgaagttaa aaataatggc ataaataata tatcaattgg agaataaaaa catatgaaaa
    10441 taaagtttat atagttttaa aaattataga attttaaaat atttatatat attctttaaa
    10501 taaaaaatta ttttttaagt gataattaag tgtttatcga taaggctatg gttgtaaaaa
    10561 tcatagcctt atttttatat tataaataaa ttagagtatt tatcaattca tagtagataa
    10621 aattgaaatt aaatataaca acaaaaaatg aaaattacaa ttaatttaat gttaaagact
    10681 caattatttg gatgtggaat ggatagtaca agtagtatat aaaaagtatt gactggacaa
    10741 ataactatat tttattttaa atatataata taatgtaata aaatatatca aatgtagaag
    10801 gtgataacct atgaaaaaaa taacaataaa tgatattgct aacttggcag gagtatctaa
    10861 aagtactgta tcaagatatc taaacaataa agatatcagt gattctacta aagagaaaat
    10921 aaagacaata atagatgaat atggatatga gccgaatgca tttgctcaaa gtttgagagc
    10981 taaaaaaaca tattttattg gtattataac accgtgttta gattcttttg taaaatctaa
    11041 aattatgatg gcaatagatg aagaactcaa aaaattaaaa tatacatcac taattattaa
    11101 tactaataga aaaataaggt cagaaataga tagtatatca aagttagcta gtctaaaagt
    11161 tgatggaata atactaattg gtaccgaaat aactaaagaa cataagaatg aaattgaaaa
    11221 gttagatata ccgatagttg tagttggaca aaaagttgat ggtatcaata gcatagtgaa
    11281 tgatgattat ggagcaggct ataaaatggg tcaatatatt gctaataaag gttacaaaaa
    11341 cattgtatat ttaggtgttg atgaaagtga tatatcagtt ggtttaaata gaaaaaatgg
    11401 agtacttaat gggttaaaag ataaagggta tgatgcaaaa gtattttata ctgactttga
    11461 ccaagaaatg tctatacaaa gaagtggaga aatgctagaa agtgaaaatc cagatataat
    11521 aatatgtgca actgataata tagcaatagc aacaatgaaa gaaatcaata agagaggtaa
    11581 aagtatacca caagatatat ctgtagctgg atttggggga tatgatgtat tgtctattat
    11641 aagccctaaa cttacaacaa taaagtttga aaataaaaat gcaggtaaag tagcagcaaa
    11701 tacgatagta aatttaatac aagaaagaaa agaaccacta ttaaaggaga ttaaatttga
    11761 attaatagaa ggtgaaagta caataaataa aaattaagat acacattaca tatattagta
    11821 atgtgtattt ttttactaaa aaaactataa ttctaaaaat taggaaaact tttttgaaag
    11881 aaatattgca tctgaaataa atttatgcta atctttaatt aaaccggttt cgaaaccggt
    11941 ttaattaaac cgatagaata gcaaaatgag tgcaaaaata actgattgta acaaagtatc
    12001 aaagtaatta ttggagtaca taggaataaa caaaatataa aagatgtagc acattgtgta
    12061 ataagactaa gaattgtttt agatagcaat aataaagcaa atataaaatt ataaaagaaa
    12121 aggaataaaa atatgtacaa agagcctaaa tatagaacca tattagaatc gacaaaagaa
    12181 gaattaaata aattaagaaa tatatcttta aatgataaat ataaaccact atttcatata
    12241 catccacaac atggactact taatgaccca aatggattag catattataa tggtaagtac
    12301 catgtatttt atcaatggta tccatatgat gcaactcatg gaatgaagca ttgggcttat
    12361 gtaagttctg atgattttgt aaactggaat agagaagatg tagcattaat accaatcgaa
    12421 agttatgaat ctcatggagc atattcaggt aatgcgatag aggtagatgg aaaattgcat
    12481 atgtattata cagggaatat aaaatatagt gcagaagata gatatgcata tcaaaactta
    12541 gctataatga acaaggatgg taaaataact aaatatgaaa ataatccaat agtaagtgaa
    12601 ataccaaaag gatatacagg acatgtaaga gacccaaaag tatttaaaag aaaggataaa
    12661 tactttatgt tacttggagc acaaacaagt gataaaaaag gagttataat agtatatgaa
    12721 tctaaaaact ctatagattg gaattttaaa ggagaattaa atgtaaaaaa catagatgaa
    12781 gattttggtt acatgtggga atgtccagat tatattaata tagatgaaaa agatatatta
    12841 atattttcac cacaaggagt agaacctaaa ggatttgatt atcaaaatat atataatgta
    12901 gtatatgcta ttggtaatat ggatttagat aatttaacat ttgaaataga tacaatgaaa
    12961 gaactggaaa aaggatttga tttctatgct ccacaaacat ttataaagga ctcacaaata
    13021 atactatttg catgggctgg aatgggagaa gttttatacc ctacagataa aaacaaatgg
    13081 gcacattgtc taactgttcc aagaaaatta aatataaaaa ataataagct attgcaaatg
    13141 ccagtagatg aattaattaa attaagatat gatgaaacaa gtgggcaaaa tactataaag
    13201 aataatatta atattataga aaatgatgaa aatgtatatg agcttaatat taatataaaa
    13261 aatattgaca gcaataaatt tgggctggaa ttattttcat cacaagatga aggtgtaaaa
    13321 ttagaattta acaaattagg aaacattgta actttagaca gaagcaactt taaaaaagtt
    13381 ttcggtgttg aatatggaac aaatagaaaa gaatatataa atattgatga aaatactaat
    13441 atcaaagtac tagcggatag aagtatttta gaaattttta taaatgaagg agaagtagta
    13501 tttactagta gaatatttgc taaagaaaat tctaatcaaa taagggttta tagtgataaa
    13561 atagtagagt atgagtatac taaatttaaa ttaaagcaag gtattgaatt ataaaaacac
    13621 attataaggg gtagaaaaga tgaaaaaagt aattagtata ggagaagctt taatagactt
    13681 tatacctaat caaaatggtg gtaaattaaa aaatgtatca gaatttagaa gagtagcagg
    13741 aggagctcca gcaaatgtta gtgctgttgt agcaaagctt ggaggaaaat cgagttttat
    13801 atctaagtta ggaaaagatg catttggtga ttatataata gatgtattaa atgaagtaaa
    13861 tgtaaatact gattatgtat tgagaacaag taaagcaaat acaggacttg cttttgtatc
    13921 attaaaagaa gatggtaatc gtgatttctc attttataga aatccaagtg cagatatgct
    13981 tttagaagct gatgaagtta aaaaagagtg gtttaataat tgtcatatcc tacatttttg
    14041 ttcagtagat ttaatagata gtccaatgaa attagcacat aaaaaagcaa tagaatacgc
    14101 acttgaaagt aatagtataa taagctttga ccctaatata agattacctt tatgggatag
    14161 tgagcaatct tgtaaaaagg caatatcaga atttttacct tttgctcata ttgttaagat
    14221 atctgatgaa gaattagaat ttataacagg agaaaatgat atagaaaaat cattacataa
    14281 actctttgtg gggaatgttg aactagtatt atatacaaaa ggaaaagatg gagtagatgc
    14341 atacactaaa aaagtaaaag gtatgtgtaa aggggttaaa gtaaatgcaa tagatacaac
    14401 cggagcagga gattcttata taggttcatt tttatacacc ttattgtata aacaaataac
    14461 attgaatgat ataaaagaaa tgaaacaaga aacgttaaat gagtatttag aattttctaa
    14521 ttattatgca gctcgtagta caactattaa aggtgcgata agttcatatg caacaaagga
    14581 agaaataact gaatttataa aaaatttata gtaggaatga gtaataggtt ttgaaggaag
    14641 taacatgtaa agagttttta aggatgttta ctatgaaaat aattaacata taaaaagcaa
    14701 taaggaggct gtctcaaaat agagataagc ctctttatca ttgttgaaat gataaaaaat
    14761 attttaatta ttgttacttt ttagaagaat atatatctta ttttaaataa gcaaattatg
    14821 taagatttat ttatattatc aatactttac ttggagtatt atgtctttag cagttatcta
    14881 attcaattga attagataaa atataataaa tttgttttgc aaattgacga gaatgttctt
    14941 ctttttcttt attcataaaa ttaggtttac ctaaatcacg ttcaacaagc attcctatat
    15001 attttaactg tgaatgctta cagtagcgct tcataccatt ttcccaaata tcagcaccct
    15061 tttcaggtct atatccacat gttgtaataa gtgctactga ttttcctgcc catagagcgg
    15121 ggcctttctc attaccatag tatttattca ttccatacac caatctgtca agtagtgctt
    15181 tcattggaga agtgcagtac caagaataaa ttggagtagc aaaaacaatt aaatcacaca
    15241 taagaatttt cttgaaaatt ttctgaacat catctttaca ataacatcca aaatcaaata
    15301 agttatcctg acacttttta caagcaatac atggctctat tttacaatca tataaccata
    15361 ttacttcaat atttgaatta taattttcta attcctcaat aaaaggtttt aaaatagcag
    15421 cagtattacc ttttttctta ggacttccca taagaatgca tacttccata ttgatacctc
    15481 cacagtattt taaaatagtt tttagtgctt aatatagttt taggatacta tgagaatacg
    15541 ttaacaacaa tacactttgt atatttgtaa taaaattgaa acaaattgtt ttatcaataa
    15601 aagttggaat gtatctataa ttaaaaatat tttaggtatg tttttacata tatgtgtgta
    15661 gttaactatg gtaatttatt gtaaaattat cttataggat tataattgct taatttatga
    15721 ttatatttaa aatcttggag gtaaaaaata tgtactcaaa tgaagactat aaatttatag
    15781 aagaaaatgc accatgttat atatatgatg agactaatat aattgaaaaa tgtaaggaat
    15841 tgaaaaataa tattttaggt tttgattttt tatattctat aaaaacaaat ccatataaca
    15901 aaattgtaaa aagcattgca gagcaaggtt ttggagcaga tgcttcttca actaatgaag
    15961 ttatgatttc atcaaaatgt ggagtttcta aagatgatat ttattattca tcaccaggca
    16021 aaacagagat tgatatagaa actgttttag ataaatgcat aattattgca gatagtatta
    16081 cagaaataga acttataaat aagatatcta aaaagcataa tcagaaagtt aaaataggaa
    16141 taagaataaa tcctgatttt actatggatg atgatagtgc taaatcaagc aaatttggta
    16201 tagatataga agatatgagc agtgttttta agatgttaag tatgtgtgat tatgtatctg
    16261 tagttgggat tcatatacat attaaatctc aggtattgga ttttgaaaag ttaggacatt
    16321 attatgaaaa atgttttttt atagctttaa atttaaataa aagtaagtat attaatattg
    16381 aatttataaa ttttggtagt ggacttggag tagcttatga taatcttaga gaaaatccac
    16441 ttaatcttaa agagctttca aaattaactc aaaagattgc ttttactaat tcaaacacac
    16501 taaaatcaag acttcttatt gaatcaggac gtttcatagt ttgtaaatca ggtaattatt
    16561 atgttaaaat tgtagatatt aaagtatcaa gagggaaaaa atatcttatt gtaaaaaatg
    16621 gacttaatgg atttatgaga cctattattt ctaatttaat tgaaaatatt acttgtggag
    16681 tagaattaga aggtcaagag ccattatata catcaaatca tgccttttct ctttcagtat
    16741 taaataattt aaaagaagaa gagtatgtaa atgttgttgg gaatctctgt acatcattag
    16801 atgtaattgc aaataatgtt aaattgaaaa aagcaaacat aggagatttg atttcaatta
    16861 gtaatgctgg aagttactcc tattctttat caccgctatt attttcaagt tatgaatttc
    16921 ctaaacaata ttataaaaga aaagatggat atattgagta aacaatagca taaattaata
    16981 aacttagtat tattgaaaaa atttaaaatg cagggggtgt aattctatat ggaattgtgg
    17041 aaaagaaatt tatttgtatg ttggataggt atgtttttta gttctatagg gatgagccaa
    17101 atagcaccta tacttccatt atatattaaa cagttggggg taactgatgt tagtttaata
    17161 caacagtatt caggtataat ttttggatgt acctttgtag tagcagcatt cttttcgcca
    17221 atttgaggga aagctgctga taaatatgga agaaaatcaa tgcttttacg tgcaagtatt
    17281 gggatgggaa tagttatatt taccatggcg tttgttcaaa atgtttatca gttattagga
    17341 ttaagaatac ttcaaggagt ttttacgggt tatgctactg catgtactac tcttattgca
    17401 acacagacag ataaaaatca ttcaggatgg gctttgggta ctcttgcaac tgcatcaact
    17461 actggctcat taattggtcc aacagtagga ggatatatag aatcaatctt aggtcttaaa
    17521 agtacattta ttataacagg aggattgttg tttgtatcat ttatcatctc tattttattt
    17581 gtagtagata attttaaacc taaagaaata aaagaatcaa tttctatacc taaagaacaa
    17641 ttaaatatat tgccaaataa atttttaatt gcatctattt ttgtaaccac attcattact
    17701 caactagctt tatactctat agaaccaatt gtaaccatat atatatcaca acttacaaat
    17761 tttgcatcaa atgtagcttt aatagctgga ttgacatttt ctgcatcagg attagcaaat
    17821 ttattagcag ctcaaaaact tggaaaaatg tcggataaaa taggtcctca aaaagtattg
    17881 ttaatttcac ttttatgggc tgggattata tttataccac aagcttttgt aaaaactgca
    17941 tggcaactga tgttgttgag atttttatta gggttgtcag ttgcagggct taatccttct
    18001 gttaacagct tgcttaaaaa aattgcacca gaagaatatg tgggaaaaat atttggatat
    18061 aatgcatctg cccagtatat aggctgttca tcaggagcat ttttaggggg acaaatatca
    18121 gcacatttag gtattagaac agtatttttt tcaacaagtt tattgttgtt taggaatgca
    18181 ctttggatgt ataaatttac tggattgttt attaaagata agactaaata atcatactat
    18241 ttacatttct aattttatga tgtaaaataa atatttttta tgtaatttat catagaatta
    18301 taaaaaaaat aaccctatgg taaaaattgg aaaatattat tatcgacaaa atctttgact
    18361 taaaaactat attgcgatag tattaattta ataaaagttt gagaggggcg taaggttatg
    18421 acaatttcaa aaataccaca agcagaatta aaagttatga agtttatttg ggaaaaaaat
    18481 gatacagtaa catcaaaaga aactattgaa gctatggaac gaaaatatgg ttggaaacaa
    18541 acaactacat tgacactttt atcaagactt gttaaaaaac attttttaag tgcagaaaaa
    18601 atagatagat acactcatta tacaatagtt gttggacata aggagtattt aagtgttgag
    18661 acaaaagatt ttttaagcaa tatacacgac aattctttac agagtttaat ttcagcacta
    18721 catgatgatg aagttgttga taaagatatg ttggatttat ttgaaagtca ttttaaaaat
    18781 ttagaagaag agtaattata tcaggataga actctaagaa tttaattaat ctatggctga
    18841 aaaccataaa tggtattatt taatcagcta ctcactattc ataaagtaat attaaatagg
    18901 ttctctaatt agtaaaatgc ctaaaataat tggaataggg agtatcaagt taaatgatac
    18961 cacttattta aataagaaat gatttttaaa atttaaaata attttataac ttgtcaatat
    19021 tcattaatct aaagtattat ttctatttaa ccatatcaaa atatatttta ctatttttaa
    19081 ttattacaat ataaacctgt cttttgttat tataggtaaa ctaaaagtaa gaaatgattt
    19141 ttatattttt aaactttatt attggtaact tatctgtaaa tattttaaaa tgtactatag
    19201 aaagttgtta aaaatattaa ataattatat taaattta
//
LOCUS       DAGSRM010000073        19189 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.073, whole genome shotgun sequence.
ACCESSION   DAGSRM010000073 DAGSRM010000000
VERSION     DAGSRM010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19189
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.073"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(959..1369)
                     /locus_tag="MJE33_002672"
     CDS             complement(959..1369)
                     /locus_tag="MJE33_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648600.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKFESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEQEL
                     EKIKTEGLKIMKPITIENEDGSQISIAYNYAAID"
     gene            complement(1585..2967)
                     /locus_tag="MJE33_002673"
     CDS             complement(1585..2967)
                     /locus_tag="MJE33_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBY9648601.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVELGNTSTENYTLKKASDSTGKNNSDEFEIMSNIE
                     NIMMLEMLGILSFIVTIMFSILLCFRYFKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGTGLVFATENKEINEVESF
                     GLTKNEQSDKIGFSISSKSTDLYSPKVESDILVSKAKSDGETHFSTAKVSVIEFREHE
                     ESSMSKVTNSENSEETYVNKSSNSKDVLYTDSGEVSIERSGYNDNRFVISRKGNLLAV
                     NKEQYRTWIE"
     gene            complement(2978..3337)
                     /locus_tag="MJE33_002674"
     CDS             complement(2978..3337)
                     /locus_tag="MJE33_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBY9648602.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSSDEITKLKQIVEKLK"
     gene            complement(3542..4321)
                     /locus_tag="MJE33_002675"
     CDS             complement(3542..4321)
                     /locus_tag="MJE33_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBY9648603.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALIVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKIGDIFVSGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTIISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICEDKDNMGVFKSMGASLDMMKGNVLKLFVLNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
     gene            complement(4782..5393)
                     /locus_tag="MJE33_002676"
     CDS             complement(4782..5393)
                     /locus_tag="MJE33_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBY9648604.1"
                     /translation="MKMVKKLILSTIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGISDALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANTLVKSYVTDEKNYDKKEKEVQDDMTK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
     gene            complement(5485..6474)
                     /locus_tag="MJE33_002677"
     CDS             complement(5485..6474)
                     /locus_tag="MJE33_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBY9648605.1"
                     /translation="MLLLKKDDIKKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNISV
                     PSQDASMLFMPGYVEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGLGGQGEPQLEAMIEARNLDEVRVFSR
                     NKESREKFAEEMNLKLSKYNTKIVAVESSDEAIDNADVIVLATPSKQPVLNGNLVKKG
                     ALISAVGSYMPDMQELCPNCLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGIDWKW"
     gene            complement(6541..7404)
                     /locus_tag="MJE33_002678"
     CDS             complement(6541..7404)
                     /locus_tag="MJE33_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HBY9648606.1"
                     /translation="MKFFKVDTKAKVLRYVFVLTLTIMAVLAIKHDYKYNNLRKDASI
                     NLNSNKEAVTVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSN
                     KIVERSTNDKIKNDIFEFYNKSYKEAKQYLKDNPDEKNKFVANVDFELKKNTDSALSI
                     KVRYYTYSGGAHGFYQDIAYNVDMRTGKFLELMDLFKDNTKYKEVIDEEIKRQIAELE
                     KKDEENIGIYNFKGIKENQNFYLQDENLVVYFDLYDITPYAAGIPEFSINKALISSML
                     KPEYVDLFDLK"
     gene            complement(7510..8106)
                     /locus_tag="MJE33_002679"
     CDS             complement(7510..8106)
                     /locus_tag="MJE33_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HBY9648607.1"
                     /translation="MERFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAI
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGYQMKLCTDKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGASGLLKMKENGSFTIGQDENSCI
                     VYGMPMVAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
     gene            complement(8146..8949)
                     /locus_tag="MJE33_002680"
     CDS             complement(8146..8949)
                     /locus_tag="MJE33_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HBY9648608.1"
                     /translation="MIKLTDEEFIVLVNHVKKEYGIDLSKKRALIEGRLYNTMIEKKL
                     SSFSQYMNLLFKDKTGNEAINLINRLSTNHTFFMREPQHFEFIQNSILPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKSVIFKPFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSSTKYKYVKPAIYKR"
     gene            complement(8985..9425)
                     /locus_tag="MJE33_002681"
     CDS             complement(8985..9425)
                     /locus_tag="MJE33_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HBY9648609.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPKADYNERTCIIITKVDEYQVGIIVDKVVDVIHI
                     ENLNLLETTNSNNTNINKDIKEIAKVEDNSILILDIRKFLINSI"
     gene            complement(9438..11540)
                     /locus_tag="MJE33_002682"
     CDS             complement(9438..11540)
                     /locus_tag="MJE33_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HBY9648610.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEKEKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEEYVSTLELSSSMKSKDNANLESTDEKEVIDSNFEYKEG
                     SDVVKIIFEQGCMMENVRAFMLVFELRDYCEFIDFYPKDVETNAESTEYIKSYGFYIQ
                     FVAKEDKAIVYKTIEDNLYVKEYINISKKEIEMKNTSNNDAVENKLKDNNKVSKSYQN
                     NYTEDEIQEDVLNEKTDMEKDSLSEENEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKATRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKSVIDNLAEPFMHLIRNAMDHAIEPIEERIAKGKEEVG
                     KIVLSAENLGGEAVISISDDGRGIMRDKILAKAKEQGLLKHQSNEYTDDEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKQDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFTLPISSIRRTFKLTDLNQIITEGNKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMNGNMIIIENEHKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNKGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            complement(11753..13456)
                     /locus_tag="MJE33_002683"
     CDS             complement(11753..13456)
                     /locus_tag="MJE33_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HBY9648611.1"
                     /translation="MKREVKLSKLAIVLLIIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQSIIFGSDYINSQEKMDSLI
                     NEFQEDIVTRSNSKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINKV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVGFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVRIAKETANALDLVVNGVEKTTNYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKESEVRESSW
                     V"
     gene            complement(13482..14441)
                     /locus_tag="MJE33_002684"
     CDS             complement(13482..14441)
                     /locus_tag="MJE33_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBY9648612.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKE
                     FIEEFIPDIILLDIILPDNSGFEFCKEVKSNARTADIPIIFITSKNTDTDIVKGFGVG
                     AIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLG
                     VVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI"
     gene            complement(14577..15095)
                     /locus_tag="MJE33_002685"
     CDS             complement(14577..15095)
                     /locus_tag="MJE33_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-binding chemotaxis protein CheW"
                     /protein_id="HBY9648613.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFKVDEQDFGVELEKIIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMRLRLKKDELMTTNCMYIVVTEIKDLVVGFV
                     VDRVDDIVSLEKSKISPPPRVTIEYSDYFVSGVGELEGKIITLLDLEKLLTDKDEVLI
                     DELINGFDCTIM"
     gene            complement(15138..15623)
                     /locus_tag="MJE33_002686"
     CDS             complement(15138..15623)
                     /locus_tag="MJE33_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HBY9648614.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKCKFADTGINELIKSMENIGANRIYIKAKIAGGAQMFLGSSN
                     SVIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTETGELLVDSINKGKKK
                     L"
     gene            complement(15620..16237)
                     /locus_tag="MJE33_002687"
     CDS             complement(15620..16237)
                     /locus_tag="MJE33_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HBY9648615.1"
                     /translation="MINYLELDDVHIDALREIGNIGSGNAITALASMINSNVEVLIPM
                     VKILEYNEATNLLGGPENKVVCILLDMKGDINGMFMFLLDESITQLMLSSLFNKEEAF
                     LDEIEAIEISAIKEIGNIMASSYVNAIASMLNMTISVSIPDICIDMVGAVLNVPMIRF
                     SDVGDKVLFIENKFKMSDNYFTSHILMIPEMSSLKEILVRLGLEV"
     gene            complement(16302..16664)
                     /locus_tag="MJE33_002688"
     CDS             complement(16302..16664)
                     /locus_tag="MJE33_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBY9648616.1"
                     /translation="MENKILIVDDAIFMRMIIKEALSKSGYNNLIEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLEVLREIKKLDSSAKVVMCSAIGQENMIVEAIKLGALDFI
                     VKPFKTETLVKVVDNALK"
     gene            complement(17408..17905)
                     /locus_tag="MJE33_002689"
     CDS             complement(17408..17905)
                     /locus_tag="MJE33_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBY9648617.1"
                     /translation="MNYEIVEINEKIVIGISKETTNKDGQAVNDIGELWKKFMGKGIY
                     NAIKGKKNDKTIGLYTDYQGDFTSPYSFVACCEVNSNSDKEKNLEELNTNNTVGESII
                     SKVIPAGKYAKFVIVGGQKEVGDFWFEFWQMDFDRTYISDFEEYQCNTFDTKKQEIHI
                     YIGIK"
     gene            complement(18080..18991)
                     /locus_tag="MJE33_002690"
     CDS             complement(18080..18991)
                     /locus_tag="MJE33_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBY9648618.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMTKGKGGGISLIEEYSIDKAILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRRNTINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKDEVFSRKIIEEVCLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDDTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLRELSKIYSL"
BASE COUNT         6192 a         3456 c         1774 g         7767 t
ORIGIN      
        1 tgatattctt atctaccatt atctatatat ttattttacg attactcttt caccctaacc
       61 ctttaactat ctattttttc ttactattct tatccacatc atccatatat ttattttaca
      121 attactcttt cactctaacc ctttaactat atattttttc tgatattctt atctaccatt
      181 atctatatat ttattttacg attactcttt caccctaacc ctttaactat atattttttt
      241 ctggtattct tatctactat tatccgtata tttattttat gattactctt ttaacctacc
      301 ccctatactt tatagtaata tgtttttaac ctttttcatt attcccaata tgctttataa
      361 ctacaaatac tctcccccct taaaaataat aataaaatta atcaataaat ttataatcat
      421 attattactc tatgtattaa gcaattacta tgccaataaa ctttgatttt tttaaacaaa
      481 tcttaaaaat cctaaaatgc taattttctt taacttttaa tgaatctaat agataacatt
      541 taattttcgt tatacttttc agaatatttc tattattatt tttaagaaaa aaaaataatg
      601 ttaacatttc ttaacataca ttaaaaaatg ttaacattat aaattgggtt taatactttt
      661 actttgactc gatttacttt tattctactt cttactttat ttttaactta gactttattt
      721 ttttattact tatttgagtc ttatttattt tattctcact ccatatgttc aattacattt
      781 gtctaaattc tttgatactt ttattacata attttaacaa gcttaagtgt tatttgggta
      841 gtgcagttga taattattta aataatcatc aactgcatgt ttatatcatt acagtagctt
      901 aatgtattta atcatactta cttaggattt tgaaatatct tagagattat atttaacatt
      961 agtcaattgc tgcataattg taagcaattg aaatttgaga cccatcttca ttttctatag
     1021 tgataggctt cataattttt aatccttcag tttttatctt ttctaattct tgttccattt
     1081 tcttaattgt ttcctcaagg tctttttgac ttacatcttt gactgcatta gggtctttag
     1141 catagtcttt taattcttga atatgtttag catactcttc ataagtataa tgctcaacac
     1201 catacttttc caattcagta gggtcagtta ttcctgattt tattagagat tcaaatttag
     1261 gtaaagcaat attagatgaa tcatttccat ttgcaaatac tgctccagtt cctcctagta
     1321 ccattgcaga taccatcatt gcacttataa gggatttttt tattttcata accaatcttc
     1381 ctttcttaga gatttatttg ggtagtgtag tttaaaatga ctattgcaat agttatcaac
     1441 cacattctta gactatcaca gtagtcatgt ttatgtcaag cctttttaaa aaacttttca
     1501 ataaaaaata ctatctcaaa acaaaattaa ttattttgag atagtatttt aattctaaat
     1561 cttttatatt actaagattt atggttactc tatccatgtt ctatattgtt ctttattaac
     1621 tgctaacaaa ttcccttttc ttgatatgac aaagcgatta tcattatagc cacttcgctc
     1681 aattgaaact tctcctgaat cagtataaag tacatcctta ctattactag atttatttac
     1741 atacgtttct tcactattct cagaattagt tactttgctc atagatgatt cttcatgctc
     1801 tctaaattca attacagata cttttgccgt actaaaatga gtctctccat cacttttagc
     1861 tttactaaca agaatatcag attctacttt gggagaatat aaatcagttg atttactact
     1921 tattgaaaat ccaattttat cactttgttc attttttgtt aaaccaaaag attcaacctc
     1981 attaatctct ttattttctg ttgcaaaaac aagacctgtc ccacaaacta atacagttga
     2041 aactaaaaaa gtaacataag ttgatttttt aaatttcata atattaacta ttctcttttc
     2101 tatttttgtc tttccaaatc cactacatat tgtcattgaa aatggattat tctcctctgc
     2161 tagacttatt agtgtggttg cataatcagc cctatattct tcaccaagta tactcataac
     2221 cttttcatca caactaaatt ctatgtcttt atttagtaaa aaaaacatca cccagactaa
     2281 gggattaaac caatggacaa caagagctac aagcataaat aactttaaca gattatcaaa
     2341 gctattaata tgaaccattt catgagttaa aacaaagtct aaagtttgat tattagcata
     2401 attcatttgc tttggcaaaa taattcgtgg ttttaaaata ccagatgtaa gtggtgtcct
     2461 agtaaattca gatatgcaaa caataaatct tctaaaacgg ctattcttac acatccattt
     2521 tttaatgaac tcatgatttt ttatatgaat tgaagtttta aaaagattaa ttattttaaa
     2581 atatctaaag cataaaagta tgctaaacat aatagtaact ataaaagaca aaatgcccaa
     2641 catttccaac atcataatat tttcaatatt agacattatt tcaaattcgt cagaattgtt
     2701 tttacctgta gagtcactag ctttcttaag agtataattt tctgttgatg tattacccaa
     2761 ttccacatca aaagtcgatt caattatact ataaatacta aaaccagaat ttaaacttac
     2821 tggtattaga agtttaaata aaacaatcaa ccacaataat acaaatacct ttttaggtaa
     2881 ttttttaaat aaacatgctc ttataaaaat aactattaaa agtaatactc cagaagtaaa
     2941 actcatttct aacaatttaa tgaacataaa tcttacctca ctttaatttt tcaacaattt
     3001 gtttaagttt tgtaatctca tcacttgaaa gattttcttt attaataaaa gctgaaagaa
     3061 atttttctga agaccctcca aacattttat taattaaagt ttctgtttcc tgtttttgta
     3121 cttcctcttt gcttattaaa gctttacaaa caaaattcgg ctcaattctc tctattgcac
     3181 cttttgacac taattttttt ataacggtat acgtagtgtt tctattccat cctgtttctt
     3241 cttttaaaat ttttactaac tctccagcct tgctttctcc tctctcccat agaacttcca
     3301 ttactttaag ttctgaatca aataatttta tctccatata aaatcgctcc tcaaatatag
     3361 ttatcacaat agtcattact ataataaaaa actatcatag tagtctatac tatgtcaatc
     3421 atttattttt taataataaa aactgtcttt ttaaaaaatt cttataaaag acagttttat
     3481 caatgttata ctttaatgtt taattaaagc catattttat ttaaatatac aactatctat
     3541 cttagtacat agtataagat ttttctttat ctaatagtat atcctctaca tctgggtttt
     3601 cttttactaa ttgtctataa aaattacaat tagtaacacc cacatatgga attaaccata
     3661 gtagacctat tccaagagta gctattgaaa gtatatacca tcctataaaa ctaaagttta
     3721 gtacaaataa tttaagtaca tttcctttca tcatatctaa actagcaccc attgatttaa
     3781 atacacccat attatccttg tcttcacaaa ttaagaaagt agcttgagaa acatataata
     3841 gcaatatata aactggtaac ataagaacaa gtattataag aactagagtt attccagtag
     3901 aaataatagt ccctattcca acattatcac ttgataaata ttcaattgct ccactagtaa
     3961 gtaaaactcc aaatgataaa aacgctccta taattgtgat tataaaagaa taaatataaa
     4021 caataattga ttcaaataaa taaaaaccaa aacttcttaa ataaattctc ccacttacaa
     4081 agatatctcc tatcttgact ttttcatctt tagtaatttt caagaacata catgtaaatc
     4141 ctgctgaaac gcaaaaagct attacagcca ctacaatagt agtcaatggt tcaaaaggta
     4201 atcctacaat tgatattaat actatcaatg caatatatac tgccatagaa attgcacata
     4261 tcccccagtt tccagataac tgagctttag atactcgttt taattcttgc cttgatacca
     4321 taaaaaacac ccctttcatt ttataaaaaa acttttaaaa atataaataa tcttttaacg
     4381 tataatatta atatatcata ttttcgacta tataagatac aattattcac catgttttat
     4441 caagatatgc cataattgta ttactagttt tatatagtca acattaccaa atacatcaaa
     4501 taaatttgat taattattca acaagtaata aaataatttc tattaattat actttattta
     4561 gtaaacaaat tatttaataa aaaaattacc taatacagta aattaattgg taagataaat
     4621 tatttattat tagtaaagta aattatttac tatttatcaa gataaattat ttataaaata
     4681 aatcatttat aaaatataat ttatactttg aaaataaaat aaactatctt ccaaacattt
     4741 tattatatgc ttaaaagata gttttgtgtc tttaaaataa attaaattgt atatattcct
     4801 tcacataact tttgtatatt actaggtggt tgccatttac catctttttt agtatatgta
     4861 atcatagttg taacttcctt agttggtttc ttattgtaaa ggtctataat catttttgtc
     4921 atatcatctt gaacttcttt ttctttttta tcgtaattct tttcatcagt tacataagat
     4981 tttactaaag tgttcgcttc actcattact ttactagtat ctaaagcttt acttacaact
     5041 ttcaaagtta tcttatcctt actagaagat tccactgtta ctgtcttttc aacaccttga
     5101 attaatttaa caattgtttc gtatatcttt tcagcatttt ctttaccata aagaggtgtc
     5161 aattgagcaa tcattgaaac cttgtccttg ctgttataag tttgaatctg tttatattca
     5221 gcttctgtta atgcatcaga aattcctttt tcatcatact tatacatttc taatgcagct
     5281 aaattagctt caaaattcga ctttactttt tcttctgcat tagaacaccc tatagttaga
     5341 actaaaacta gagccataat cgtagataat attaactttt ttaccatttt catacaaaca
     5401 tcctccctca attaaaataa taaaaaattt atttatcaac attataacat aaatttgtct
     5461 aactatagaa ttattttgaa tattttacca cttccagtct attccaatac cgttttcttt
     5521 tgctttatcg tatattcttt ttgctgttac gacatcttgt acacctatcc caacagtttt
     5581 aaatacaatt atctcatcat catcttctct tccaggaata gttccgttta taacatttcc
     5641 taaatcacca ctaaaatcat ctttacttat cttaccttct tttaatggaa ttaatatatc
     5701 tcctgactca gataaaactg catctgttga ttcaaaaaat atcttagacg ctctagttaa
     5761 acagtttggg cacaactctt gcatatctgg catatatgaa cctactgcac ttattaaagc
     5821 tccttttttc actaagtttc cattaagtac tggttgtttt gatggagtag ctaaaactat
     5881 cacatctgcg ttatctatgg cttcatcaga tgattcaact gcaactattt tagtattata
     5941 tttacttaat tttaagttca tttcttctgc aaatttttct ctactttcct tgtttctact
     6001 aaaaactctt acctcatcca aatttctagc ttctatcatg gcttcaagtt gtggctcacc
     6061 ttgtcctcct agacctatca aagctccaat cttagaatct tttttagcca atacatctat
     6121 agctgctcca gaggctgctc cagtacgaat ctgagttaca taagtaccat ccaatactgc
     6181 actaacttct ccagtttctc catctaataa tagtactgtt gctggaatta ctggtttgcc
     6241 tttttgagca tttttaggaa atacagatac aatttttatt cccccacagc ctaattcttc
     6301 tacatatcct ggcataaata acatactagc atcttgactt ggaacagata tattggttct
     6361 caaaggattt acactctttc cttcacaata aattctaaaa gcttccttat cagcttcaat
     6421 tgcatctctc attgtaaata ctttttttat gtcgtctttt tttaaaagta gcatttttat
     6481 acccccaatt ttaataagtc tctatgctaa atattatagc ataacattta atttaatctt
     6541 ctatttcaaa tcaaataaat ctacatactc tggttttagc atagaactta tcaacgcttt
     6601 atttattgaa aattctggta ttcctgctgc atatggagtt atatcatata aatcaaaata
     6661 tacaactaga ttctcatctt gcaagtaaaa gttttgattc tcttttatac ctttaaaatt
     6721 atatattccg atattttctt catccttttt ttcaagttca gctatttgtc ttttaatttc
     6781 ttcatctata acttctttat atttagtatt atccttaaac aagtccatta attctaaaaa
     6841 ttttccagtt ctcatatcca cattatatgc tatatcttga taaaatccat gtgctccacc
     6901 actataagta taatatctca cttttatact aagagcagaa tcagtattct ttttaagttc
     6961 aaagtctaca tttgcaacaa acttattttt ctcgtcagga ttatccttaa gatactgctt
     7021 tgcttcttta tatgatttat tataaaattc aaatatgtca ttttttatct tatcatttgt
     7081 agacctttca acaattttat ttgaatttgt tattacaggc atgtttatag tactttctaa
     7141 atactcatca tttttcacta ttgttttact tcctattgta cttactgata aagtttgttt
     7201 actatttgta ttaccctttg gtaatgactg gttactcata acagtcactg cttccttatt
     7261 actatttaaa ttaattgaag catctttcct taaattatta tatttatagt catgtttaat
     7321 tgccaaaaca gccattatag tcaatgttaa aacaaataca tatcttaaaa ctttagcttt
     7381 tgtatcgact ttaaaaaatt tcatattatc attttcctcc taaatataaa tctctcttat
     7441 tatatttgga gtttctaggg ttttttatcc atataattta ataaaagatt aatttttatt
     7501 tataaaaaat taatcttttt gttttaaaat attaatatgt cttatcaaac agtttgaaat
     7561 ttcaccaaga ggtaattgta acataacaga acctctatca aaagctacca ttggcattcc
     7621 atatactata caagaatttt catcctgtcc aattgtaaat gaaccatttt ctttcatttt
     7681 taaaagtcca cttgctccat cataacccat acctgtaagt attattccta ttgaattttt
     7741 acctgctact tctgctactg aatcaaacaa aacgtctact gatggacagt gaccacttat
     7801 tctttctcct ttttcacaag ttacataata tccttgttta tccgtacaca atttcatctg
     7861 gtaaccccca ggtgcaatta acacatttcc ttgttctact ctatcattat tcttagcttc
     7921 tcttacattc ataatacact gcttgtcaag ccgttgtgca tacatataag taaatatagc
     7981 tggcatatgt tgaactacaa caatccctgg tgttgatttt ggtaggtctt ttataatttc
     8041 aagtattgcc tctgtaccac ctacagatgc gcctatagca ataaccatat tattaaaacg
     8101 ttccacatta ccgcctccaa aaaatcatat atcaaaaata cctttttatc gtttatatat
     8161 agcaggtttt acatatttgt atttagtact acttctgttg attacttctg catgtcctac
     8221 aaacaaaaaa ctatcttttt tggtagcatt ataaaattta tttataagtt cttctcttgt
     8281 ttttaaatca aaataaatca taacatttct acaaaaaatc aaatcaaaat gttgatatga
     8341 gaaatcattc attaaattaa acggcttaaa aattacactt tttctaatct tatcacaaat
     8401 tttaaatgta ccatccttgt tgtctataaa atacttcttc ttccataact caggaacatt
     8461 gttaacattt gattctatgt atatacctct tttagctttt tcaagtgagt tagttgaaat
     8521 atctgtggct atgattttaa tgttccacaa ctctttatta tatccaaaat aatcatccaa
     8581 agtcatagct attgaatacg cttcttctcc actagaacat cctgcactcc atatatttat
     8641 gtttcttatt ttattatttt cttcccaaaa aggaagtata ctattctgta taaactcaaa
     8701 atgttgaggt tctctcataa aaaatgtgtg gttagtactc aatctattta ttaggtttat
     8761 agcttcattt cctgtcttat ctttaaataa tagattcata tattgactaa aagaactaag
     8821 ttttttctct atcattgtat tgtataagcg accttcaatt aacgctcttt ttttacttaa
     8881 atcaatacca tattcttttt ttacatggtt tacaagaact ataaattctt catctgtgag
     8941 ttttatcatg caaaaacttc ctcctgctaa tttatcagaa ctaactatat gctattaatc
     9001 aaaaactttc gaatatctaa aattaatata gaattatctt caactttcgc aatctctttt
     9061 atatctttat taatattagt gttgttagaa ttagtcgtct ctagtaaatt tagattttct
     9121 atatgaataa catcaacaac tttatctaca ataattccaa cttgatactc atcaaccttt
     9181 gtaattatta tacaagttct ctcattataa tcagctttag gaagattgta ctttatatgc
     9241 atattaataa ctggtattat tcttcctctc aaattaataa ctccacttat atatgttgga
     9301 agacatggta catacgttat tgattgaaat cttatggttt caatcacatt ttctaattct
     9361 attccataaa taacatcatt gacataaaat gttaatactt gtttaataat ttcatcttta
     9421 gccatacttt aaatccccta atatatttct ataatgctat ctgcatctat aattaaactt
     9481 atacttccat ccccaagtat tgtacatccc gataaaccct tattttttat attaaatcgg
     9541 ttaaaataaa ctggaaatgg tttcacaaca acttgctgat tacctattat ttcatcaaca
     9601 aaaatgcata ctgctttatg ctcattttca ataattatca tattaccatt catcagattg
     9661 gttacttttg tttgaatatt atatatttta tgtaacctta taatgggaat acatgctcca
     9721 tatattgtaa ttaactctgt cttatttcct tctgtaatta tttgatttaa gtctgtaagt
     9781 ttaaatgttc ttcttatgga tgatattggt aaagtaaata gtgaatctcc aactttaaac
     9841 ttcataccct ctactataga aagagttaat ggtattttta tagtaaaggt tgtcccttca
     9901 tcctgtttac tttttacttc aattgaacct ccaacttttt caatactttt acgaacaatg
     9961 tccatgccta ctcctcttcc agaaaattca gttatagatt cttttgtaga aaagcctgga
    10021 gtcataatca aagaattgac ctcgtcatca gtatactcat tactttgatg tttaagaaga
    10081 ccttgttcct tagcttttgc caaaatctta tctctcataa tgcctctacc atcatctgaa
    10141 atacttatca cagcttctcc gcctagattt tcagcagata aaactatttt tcccacttct
    10201 tctttacctt tcgctatacg ctcctcaata ggctctattg catggtccat agcatttctt
    10261 ataaggtgca taaaaggttc tgctaaatta tcaattacac tcttatctac ctctgtctct
    10321 gctcctatag ttattaactc tacattttta tctaattttt tattcatatc tcgtacaatt
    10381 ctatgcattt tttgaaatac accagcaatt tgaatcatac gcatagacat tactatatct
    10441 tgcaattcat ctgtaagctt gtgtaattgt cttgttgcct tattgaaatt atctatatca
    10501 acacctgtag aatttacgct atttacaacc attgattcca taattacaat ttctccaact
    10561 atattttgaa gtaagtccaa tttttctaaa tttacactca taatactctg cttacttatt
    10621 ggtaaatgtt cattttcttc acttagacta tctttttcca tatcagtttt ttcatttaaa
    10681 acatcttctt gtatctcatc ttctgtatag ttattttgat aacttttact tactttatta
    10741 ttatctttta atttattttc tactgcatca ttgtttgatg tatttttcat ttctatttct
    10801 tttttactaa tatttatgta ttcttttaca tagagattat cttctatagt tttataaact
    10861 attgccttat cctctttagc tacaaattgt atataaaacc cataactttt aatatactct
    10921 gtagactctg catttgtttc tacatctttt ggataaaaat ctataaattc acagtaatct
    10981 ctaagttcaa atacaagcat gaaagctctc acattttcca tcatgcaacc ttgttcaaag
    11041 attattttta ctacatcaga gccttctttg tattcaaaat ttgaatctat tacttctttt
    11101 tcgtctgtac tttctaaatt tgcattatcc tttgatttca tagaacttga taattccaaa
    11161 gtactcacat actcttcaat tccttcaata aaattagagt aatcagaaga tatacaacct
    11221 cttgattgta actcatctat atctcttctt ataaaatcta atgattgaaa gactaaatca
    11281 aataataggt cattgtctaa aacttctatt ttaccttcac gtatagcata aaacaagtct
    11341 tctacactat gtgctaaagc agatatattg tctaacccca tcatagatgc tgaacctttt
    11401 atcgtatgca ttattctaaa agtttcatta atgttgttat gagtaaatac tttctctttt
    11461 tctatttgaa tcataatttc atctaattgt tcgagtaata aacttgtctc aaatataaac
    11521 atatctaaca tttgtctcat actcggttca atataattca catttatcac ctacgaccta
    11581 caaataattt tctattatat atgcatctca taatttaaat ccttgatgat tctcttatct
    11641 cactatatgt acctcataat ttaaatacac aattattctc ttacttcaat atatatacat
    11701 ctcataattt aaatacatga taattctctt acttcaatat tcacctcata atttaaaccc
    11761 atgatgattc tcttacttca ctttccttta gatgaaattt ttctactaat tcttttaaaa
    11821 tttgagcttg agcagacatc tcttctgagg ctgctgcact ttcctctgct gttgctgagt
    11881 tcatttggac tactatggaa atttgctcta ttccagataa aatctgtgta attgctactt
    11941 cttgactatg tacagccgaa gaaataccat taatataatt agtagttttt tctacaccat
    12001 ttactaccaa gtccaatgca tttgcagtct ctttagcaat tctgacacca ttttctactg
    12061 ttttaatagc ttcttctatg agaatagctg tattttgagc tgcttttgca cttttaccag
    12121 ccaaagacct aacttcatct gctacaactg caaatcctct accatattga cctgctcttg
    12181 ctgcttcaac agctgcattt aatgcaagaa tattagtttg gaatgctatc tcatctattg
    12241 ttttgattat ttttgatatt tcactcgatt tttcacttat ttcacccatt gatgtcatca
    12301 tatcatccat acatctactt ccgaaaccaa cttgtgatgc agattggtca gatacctcac
    12361 atgcaagact agcactatct gcacacttct taattccatc tgatatctca ttaatcgtgg
    12421 aagctaattc ttctactgaa ctagcttgtt cagtagcacc ctgtgaaaga gcttgtgcac
    12481 ctgctgcaac ttgttctgaa gcacttgcaa cttgctctgc tgataaattt atttcagaaa
    12541 aatcaccatt aaaagagttt attatacttt ctaaagatat tttaattgat ttaaaatcac
    12601 ctataaattc ttggtctatc tctactctta aatctccttt tgcaatacta gttaatgtac
    12661 tgtctataag gtctatgtaa tttaaaagca tactttgcat ctttttgaca gaactcacta
    12721 atagtcctat ttcagattta ctttcttcaa cgtctatact tgttcttaag tccccagatg
    12781 caattcttga aaagtgtttt tctactttat taataggaat cactattttt aattttatta
    12841 ctgtatattc tattattgtc aatattatta aaagggaaag tataaatatt gttcttaata
    12901 ttagactttg taattctttt tttacttcta caattttgga atttgaacgg gtaactatat
    12961 cctcttgaaa ttcatttatt aaagaatcca ttttctcttg ggagtttata tagtcactac
    13021 caaaaattat actttgagct ttactcttat cattactttc gactgccttt attgcacttt
    13081 gttctgttgg tactaaagaa tctgatattt cttttgcttc taagatttta tttgactcgc
    13141 tctttgtaag accaattttt tctaatttgt ttaaagctga ttctcttgtt tttccgtata
    13201 attctttttc ataaatatct ttattctttt tttctcctgt aaatacatat cttcttaata
    13261 catcacttat ctctctagtt gtatctttta tatactgact ttgagtcctc aaattattca
    13321 tatcattaat acttttttct actttttcaa atgctttata agatgaaata gaagaaaaaa
    13381 tccctgctag aacagccaat actataataa tcagcaatac aattgctaat ttagaaagct
    13441 ttacttctct tttcatatct gtactccttc ataaagtaag tttatatctt ttttaatcct
    13501 ttcattgtac tcactacgct acaatctttt cctaagtttt taccctcata cattgcttta
    13561 tctgctaagg atatgttttc ttctaatgat atatttgagt ttacttctac tactcctaat
    13621 gtaatagtgc actttatttt atgttcttta tagttgaaat caaactcctt tatctcttta
    13681 cggattcttt ctgaaagtat cttagcacct tctattggag tatttgtgag cattatcaaa
    13741 aactcttctc caccccatct tgatacagta tcaatttgtc tacaattctt ttttattata
    13801 cttgatatta ctgttaacac gaaatcccct gcttcatgcc cataagtatc attgataacc
    13861 ttaaagtcat caatatcacc aaggataagg gatattgatg tttcactata tttattaatt
    13921 cttctttgct ccataagctg attaattata tgtcgcctat tgtaaagacc tgtaagataa
    13981 tctcttacaa cgagtctatt caatttttca agagaagatt ccagttcatt atttatcttt
    14041 tttagtttat cttgagattt ctttacttca agatgagcta taacacgagc ttttaattct
    14101 gtcatactaa aaggtttcac catatagtca attgctccta caccaaatcc ttttactatg
    14161 tctgtatctg tattttttga agttataaaa attataggta tatctgctgt acgagcatta
    14221 gattttactt ccttacaaaa ctcaaaacca gaattatctg gtaaaattat atcaagcaaa
    14281 ataatgtctg gtataaattc ttcaataaat tcttttgcct ctattgcatt aaatgctttt
    14341 ttaaccttaa aattatcttt tgtaagtgtt ttatctatta agtcacacat cagaacacta
    14401 tcatcaacga ctaaaattgt tccctgttgc ttatttttca tatacaaacc tctcttaaac
    14461 ataatatatt caaattttaa tgatttaaaa tttttatact taataattat accactatat
    14521 tcttaaatgt tctcatcaca atctttttat aataacactc caactacatc cctatattac
    14581 atgatagtgc aatcaaaccc attgattaat tcatctatta aaacttcatc tttatctgtc
    14641 aaaagtttct ctaaatctag taaagtaatt attttacctt ctaattctcc aactccactt
    14701 acaaaataat ccgaatattc tatagtaact cttggtggag gtgatatttt acttttttct
    14761 aagcttacta tatcatcaac tctatcaacc acaaatccaa ctaccaagtc ctttatttca
    14821 gttactacta tatacatgca atttgtagtc attaattcat cttttttaag tctcaaacgc
    14881 atatctatta tcggaatgat ttttcctctt agatttatta tgcctttaga atatgtaggt
    14941 agttctggaa taaatataat ttcttgattt ccagtaattt ctattatctt ttcaagttca
    15001 accccaaaat cttgctcatc tactttaaac tttagatact taattccttc atcaattagt
    15061 ccataatcta aatatgggtc atctggaaat ctcatttaat acctccctcc taaaatacac
    15121 tttctatgaa atatatatta taatttcttc ttacctttat ttattgaatc tacaagtaat
    15181 tcacccgtct cagtgtaaaa acgtattgtt ctaccataat tcatacctgt atcctcagct
    15241 aatattggaa tatttaatcc taaaagagtt ttttttactg cctcaacatt cctatgtcct
    15301 atacttgcaa ttactgaatt gctacttcct aaaaacatct gagcacctcc agcaattttt
    15361 gcttttatat atattctatt tgcacctata ttctccatag atttaatgag ctcatttatt
    15421 cctgtgtcag caaatttaca cttattttca atgttaatag catctttact atatggcaac
    15481 ataacgtgta acattccacc aattccagct actttatcta tcaaacatac tcctacacaa
    15541 gaaccaagtg cataagtaac taaaacattt ggtctatagg ctatcttcat atctgcaatg
    15601 ttcactacta tctctctatt catacctcta atcccaacct cacgagtatc tctttaagtg
    15661 agctcatttc tggaatcatc aaaatgtgac tagtaaaata attatcactc attttaaatt
    15721 tattttctat aaataagact ttgtcaccaa catctgaaaa tcttatcata ggtacattta
    15781 gaacagctcc aaccatatca atacatatat ctggaattga aacacttata gtcatgttta
    15841 acatagatgc aatagcattt acatatgaac ttgccataat atttcctatt tccttaattg
    15901 ctgaaatttc aattgcctca atctcatcta aaaaagcttc ttctttatta aaaagagaac
    15961 ttagcataag ttgagttatt gattcatcca acaaaaacat aaacattcca ttgatatcac
    16021 ctttcatatc gagtaaaata catactactt tgttttctgg accaccaagc aaatttgtcg
    16081 cttcattata ttcaagtatc ttgaccatag gtattaatac ttctacattg ctatttatca
    16141 tggatgcaag tgcagtaatt gcattcccag agcctatatt accaatttct ctaagagcat
    16201 ctatatgaac atcatcaagt tctaaataat ttataatata aacccctccc tttattattg
    16261 catttatttg cttttcattg tattagtgta tatttacact tttattttaa agcattatct
    16321 acaactttaa ctaatgtttc tgttttgaag ggcttcacta taaaatctaa cgctccaagt
    16381 tttatggctt ctacaatcat attctcttgt cctattgcag agcacattac aaccttagca
    16441 cttgaatcca acttttttat ttctcttagc acctctaaac catctttttt tggcattgta
    16501 atatcaagca acatcaaatc aggcttttca tatttaaata tattacacgc ttcttctcca
    16561 tcatttgctt ctattaaatt attatagcca cttttactca gagcttcttt aattatcatt
    16621 ctcataaaaa tagcatcatc tactatcaaa attttatttt ccataaaact aactcctcca
    16681 tatgtacaac aataaacgct tttatcttca gttattgtct atactatctg actatttttc
    16741 gctacacaat aaatacattt cattgataaa taaaagtaat tatttatgcc atattatact
    16801 taattctctt aacataattt gtcaacaaaa aaacttatat atctattgta atatatgttt
    16861 ggcgttttga cgagttatta aaaatataat tgtataaaac ataaaaagtt atcaaaagta
    16921 attaaattat aataaaacaa ctttatactt tttttatgtt tgtataaaaa aaattttcag
    16981 attcattaaa atcatttctg tagattcata taggatttga ctttctcaat atctaataga
    17041 tagttgttta tttacttaat tatatttctc tatatgtatt atcgattaaa ttagatttag
    17101 ctttataaat tattttttat atttttcaaa ttgtatactc ataaatccag aagtattcta
    17161 atgtgtttat aatacttttt ttcatatttt aaattaaata acaaatcatt atatttacaa
    17221 gtatcattta tcaataaaca atattaaatg aagaatatgt ctgaagattc aaagtgatac
    17281 tgatactatt aattactaaa aaagcagtaa atcttttaca gatttactgc ttatagttat
    17341 aaataataaa actatctata ataaatgtac tatactcaat atttcattca atatagtaat
    17401 aacaaatcta ctttattcct atgtaaatat gaatttcttg tttctttgta tcaaaagtat
    17461 tacattgata ctcttcaaaa tcacttatat aagtcctatc aaaatccatt tgccaaaact
    17521 caaaccaaaa atctcctact tctttttgac ctcctactat aacaaacttt gcatacttac
    17581 ctgctggtat aactttacta ataatacttt ctccaacagt attattagtg tttaattctt
    17641 ctaaattctt ctctttatca gaattactat ttacttcaca acatgcaaca aagctatacg
    17701 gacttgtaaa atcaccctgg taatctgtat atagacctat tgtcttgtca ttctttttac
    17761 cctttatagc attatatatc cctttgccca taaatttttt ccacaattca cctatatcat
    17821 ttaccgcttg tccatcttta tttgtagtct ccttcgatat tcctataact attttctcat
    17881 ttatctctac tatttcataa ttcatacttt atacctcact ttgtttttat taatctattt
    17941 tattttgaac taattcaatt ataatttata atatacgaca cctttatgtc atatttttaa
    18001 tataattaaa ctcagtgaga tattatatac taaatgcaag atattatata tttaataaaa
    18061 tctttaatct agcattatat tataatgaat aaattttaga caactccctt agtctagtta
    18121 ttacattatc cctaacatct ttaggttcaa gtacttcaac attgtcacca aatgataaaa
    18181 tatatccata aatccattca ccaataggaa attctgtaat tacatcatat gtatcatctt
    18241 catttttagt tattaaatcc ttattaaaat catcaaatac tctaaaaagc atcttttctt
    18301 taaacctcaa tttcaacgtt atagtatttt ccttaattac tttagaatca ttcaaacata
    18361 cttcttctat tatctttctt gaaaatactt catcctttat agataaattc cttatcctac
    18421 taattctaaa tatcctaaaa tcatctttta gtttacaaaa tccatacaag taccaagtct
    18481 tactcttata cataagcttc agtggttcga cagttctgtt tgtctgactt gacaaactat
    18541 ttacataatt aaaatttata gtattacgcc ttaaaatagc ttcttttatt tttgtaaatt
    18601 tatcatcttc attaaaatta cttccccatt cagtaaagtc tatttcaatc cagtttgaaa
    18661 aattctgctc tccaaatatt gagccaattt tatttactac tgaatttatt tctggatact
    18721 tagtcgcctg taatgttttc agtgcaacta ttaaactctc tttatctttt tttgacaata
    18781 tagccttgtc tatagaatat tcttctatta aagatatccc tcctcctttg cctttcgtca
    18841 tatatactgg tactcccgac atagacagag tttctatatc tctatatatg gttcttgtag
    18901 aaacttcaaa tctatctgcc aactctttag ctgttactaa ttttttattt aatagaatta
    18961 ctattatttc tgttaatcta tttattttca ttcattacca cctactaaaa ttattaaatt
    19021 tatttaatct aaattaatca tactaatttt taaataaata aaatcttaga atcttatcta
    19081 aattattttt acatgatttt atttactttc tattatcttt ctcactttgg atattatcat
    19141 cttaatttat ttattactat atcaatttac ttattatcat ctcatttgt
//
LOCUS       DAGSRM010000074        19161 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.074, whole genome shotgun sequence.
ACCESSION   DAGSRM010000074 DAGSRM010000000
VERSION     DAGSRM010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19161
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.074"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            20..439
                     /locus_tag="MJE33_002691"
     CDS             20..439
                     /locus_tag="MJE33_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016282109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HBY9648619.1"
                     /translation="MNKLYSKNHNEAYKTFLFLENESLKSNITYCFFDSFLEMIDNEN
                     SYIRTRGLLLISANAQWDIDNKIEINIDSILSHIVDKKPSVSRMFIKSIPNITKYKEN
                     LINRIKMELSNADISIYNNNMKPLVEKDINDTLSNIS"
     gene            1098..2405
                     /locus_tag="MJE33_002692"
     CDS             1098..2405
                     /locus_tag="MJE33_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648620.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKADIGCVEEIDNEDDNLALDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVVTIDKRY
                     DYRKELKSELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYYNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYVILVEQNKILTDEVFDCNLASKNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRKTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     misc_binding    2491..2753
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2879..3682
                     /locus_tag="MJE33_002693"
     CDS             2879..3682
                     /locus_tag="MJE33_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBY9648621.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVV
                     VGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINETLKEMKKDGTFDKIYDKWFK"
     gene            3720..4331
                     /locus_tag="MJE33_002694"
     CDS             3720..4331
                     /locus_tag="MJE33_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBY9648622.1"
                     /translation="MLQGLEIVIAMFCITLIVSIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRVIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            4363..5034
                     /locus_tag="MJE33_002695"
     CDS             4363..5034
                     /locus_tag="MJE33_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBY9648623.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMAKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
     gene            5332..5694
                     /locus_tag="MJE33_002696"
     CDS             5332..5694
                     /locus_tag="MJE33_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBY9648624.1"
                     /translation="MIKLYGYTKCSTVKKAKNWLKENNLEFEDIDMVQNPPSKEELKS
                     IYKTSGYDIKKFFNTSGMKYRELGLKDIVKTESDDKLLEILVSDGMLIKRPLLLDGKN
                     VLLGFKEDVWKSTLLKED"
     gene            6130..6831
                     /locus_tag="MJE33_002697"
     CDS             6130..6831
                     /locus_tag="MJE33_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HBY9648625.1"
                     /translation="MSIEDLYDFECVPLNNEIDIDVLFEKMIEDGKEKEYTPIIVIDE
                     KVGLLRKNIDMITKEYGSLENYREYCLTKYNEIDVNQFFDFRKSEISELLKELIDLED
                     DFYDYKHFRPIMNIDIFHEYNKVYIAKIPTIKPYEVFAYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKNLTKDGKKFDDLCIELVFYCEDIIIQGYETLKAL
                     KDVLKRSTIVLFWWD"
     gene            7101..7919
                     /gene="larE"
                     /locus_tag="MJE33_002698"
     CDS             7101..7919
                     /gene="larE"
                     /locus_tag="MJE33_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HBY9648626.1"
                     /translation="MEVNFVKEKEKLDKLKKMLLELGSVVVAYSGGVDSNFLLKVAKD
                     TLGENVIAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGVDRCYH
                     CKKYIFSKIKEVAKEHNIKYIVDGTNIDDLGDYRPGLKALSELGVISPLKDSSLKKEE
                     IRSLSKTLGLNTFNKPSFACLASRIPYGVEITDENLRIVEKSEEYLSNLGFSQFRVRM
                     HGDIARIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            7974..9122
                     /locus_tag="MJE33_002699"
     CDS             7974..9122
                     /locus_tag="MJE33_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBY9648627.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRTLDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            9258..10646
                     /locus_tag="MJE33_002700"
     CDS             9258..10646
                     /locus_tag="MJE33_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBY9648628.1"
                     /translation="MKVLKLITVVALMMLFVTGCNIKTESPEQLAKIPDYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMHIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGK
                     MKIKDSVKFENVKNLSELYITLGNVASSVGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGSTGNNKNTYSSKSSATIS
                     WYRWNGKEGADSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTGTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            10675..11373
                     /locus_tag="MJE33_002701"
     CDS             10675..11373
                     /locus_tag="MJE33_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY9648629.1"
                     /translation="MKEKILILEDEIGIRSFVSINLKREGYEIVEAGTGREAIEKMTT
                     EKDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDKIEGFISGADDYI
                     IKPFSIKELLVRVSALLRRVAKDDSSVKSSEIVSPPFILDIDKRKLFKNGKEIELTPT
                     EFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRNKIEDDPSKPKYI
                     QTIWGYGYCFRKEE"
     gene            11376..12809
                     /locus_tag="MJE33_002702"
     CDS             11376..12809
                     /locus_tag="MJE33_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY9648630.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVASQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIESEKNVGTKITFNIKNKFAKQA"
     gene            12831..13568
                     /gene="truA"
                     /locus_tag="MJE33_002703"
     CDS             12831..13568
                     /gene="truA"
                     /locus_tag="MJE33_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBY9648631.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHS
                     RYNVKSKTYMYKIDNNKYHNPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
                     KSKKKSNVREIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIK
                     YMLESKDRCQSSDTAPAKGLCLWKVTY"
     misc_binding    13679..13886
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            14139..15338
                     /locus_tag="MJE33_002704"
     CDS             14139..15338
                     /locus_tag="MJE33_002704"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HBY9648632.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGASKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWDIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
     gene            15585..16631
                     /locus_tag="MJE33_002705"
     CDS             15585..16631
                     /locus_tag="MJE33_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4872 domain-containing protein"
                     /protein_id="HBY9648633.1"
                     /translation="MRKIIEIKHHSHDYECMWNGIEDLYINKTGEYLPNDFFFLLSGF
                     GSFCYMKTNKADLKRMVALGDGRTKQMYKFLAPIVGFEYKHHEFKKYEQALKKAKSEI
                     DDGYPVILGALDMYYLPYYKKLYHKEHIPFHYILMVGYDDEGIYLYDCGRTELLHLSY
                     DKLRESMNCNSPGLSKENTICTIRMNTSKNKYNIAREAIAIKRDMFLNPPKGFLGYKG
                     FEKFIKELPQWNTELDKDDYDKILTNMVMFFGTVPTIPNAIKGIKEPDTVQFKGGFDK
                     MSRMLNSIGEEYKNNSFIQVADIFDKTAIIIEKISNIIIDYLTQTCDDTEQLPLLFSE
                     VLEHMKTGYLILDV"
     gene            16739..17902
                     /locus_tag="MJE33_002706"
     CDS             16739..17902
                     /locus_tag="MJE33_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBY9648634.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKPNKITDAGYRLYDDES
                     IKTLQQILFFKEIDIPLREVKEIMSSQYFDKVEALKNQKKLLILKRKRLDELIELINQ
                     TLRGEGNIDFKEFDMSEYFNVLEEFKREHRNKVIKIYGSVEKYNEYIERVKSNEEKIA
                     KMAIKQYGTIEKFAKAIKTNFSSDILNLGEKFDRYKNDCLKEKHPKLKELYRKLVEDL
                     SRNPSSTDLQEIAKAITDISKKDYEIFSMDTGDDNWYYMVQNYLVNPMWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSREVQSIVEEIDNEMKRSNE
                     FYKIDNGERYFDYMSELYLQDSNYIKVTDKNYGDGASKFIGEAFKIYFDNNNC"
     gene            complement(17991..18371)
                     /locus_tag="MJE33_002707"
     CDS             complement(17991..18371)
                     /locus_tag="MJE33_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HBY9648635.1"
                     /translation="MKSTTFVILWTILFVLFGFYVNNKLYDFTEGYKDNISVLEKSIE
                     NEDWEKAQKEADSISTSWNKEKNHWYKVLNHEYFDEIGLRFNILDKAIYTENKLKSLE
                     EVESIKTYLGNIIESVKFDVNYIF"
     gene            complement(18374..19033)
                     /locus_tag="MJE33_002708"
     CDS             complement(18374..19033)
                     /locus_tag="MJE33_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBY9648636.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAISGLVIMQTLISFLSLKSRKLSSFLSGKPSVLIDKGKIVY
                     KELKKERISIDELLEQLRIQGYFNLKDVQYAILETDGNLSVVPASSYNSTPPRAFNHL
                     PIPLILDGRIINKNLDIAQKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
BASE COUNT         7749 a         1836 c         3275 g         6301 t
ORIGIN      
        1 gaagaatctc aatttactta ttaataaatt atattcaaaa aatcataatg aagcatataa
       61 aactttttta ttcttagaaa atgaaagttt aaaatcaaat attacatatt gtttttttga
      121 ctcattccta gaaatgattg ataacgaaaa ctcatatatt cgaacaaggg gattgttatt
      181 aatctctgca aatgctcagt gggatattga taataaaatt gaaataaata tagatagtat
      241 actaagtcat atcgtagata aaaaaccttc tgtatcaaga atgtttatta aatccatacc
      301 taatattaca aagtataaag aaaatcttat taatcgcata aaaatggaat taagtaatgc
      361 agatataagt atttataata ataatatgaa accattagta gaaaaggata taaacgatac
      421 attatctaat attagttaaa tattaatata gaaattgaat attatctagt tacaattatt
      481 tataactaag ataataaaac aacaatggtg ctaatttata tgtataaatt aattatgaaa
      541 aatttatata agtaaaatat attaacataa aggtaaaatt ttgtatacta aaagggaata
      601 tttacagttt aatggcagtt ttaaaataat aaattaaagc tgtcattttt atttaaaaat
      661 ttttagaaaa agtacaatag tgttaatgtg tgaattagat aaggtgtata taatagttta
      721 gatgatatga tataaaatat gttgtaaatt aaatgttttc tttgacaata tatacacaag
      781 gatgataata tctatatgaa taaaagatag tgtgtaattt gataatatgt aattataaca
      841 ttatgatata attacaataa agtatgtaat aatctgtaga aatttcaagc tagttatcta
      901 agttttcgga taactaaatc taaacggaat acataatgta ttaaataaat gatgatacta
      961 aaatgatact atttttatag aaatttattg aggtatataa ttaatgaaaa taatatatta
     1021 aattatataa attaaattat ataaattaaa ttatataata tattgagttt tacataaaag
     1081 caaaggtggg aataaaaatg aaagaaattg ctgatgctat aacatcagag tatttaagct
     1141 atggtatcaa tgaagatatt agtatctatg aaggtagatt ttgcatatat ttagataaaa
     1201 aatatagatg taatgggaaa atttattata aaatgacacc tccaatatct ataagtttta
     1261 aagcagacat aggttgtgtt gaagagatag acaatgaaga tgacaattta gctttagatt
     1321 atgataatgc tattcttgaa gttcatggat ataaaattat ttctataaca ataaatactt
     1381 taagtgaatt tagtgtagaa ggttatatca atgatgactg cataaaatct aagaactcct
     1441 atgttgaata tgtagatttt aatataataa acttagataa aatacctgga aaactcatta
     1501 aatacaatga taaggtatac gcaggtagaa ttgaatttga tataaatgat tatgttgtaa
     1561 ctatagacaa gcgatatgat tatagaaaag aattaaaatc agaattaaag tctaaatctg
     1621 gagcaataat aactcatata ggtaggatta gaagaaaaga tggtagaatt tttagaacaa
     1681 ataatacaat aaatctttta gataggatat caacagcttt gagttttatg tgtggaagat
     1741 atgtagggtt ttgtttggcc aaagggtata gaagtggtaa tgaagtttat agaatatgga
     1801 atgaaaatca aatatctcct tttagatatg taccaacatg gtcagataca ctttcaaatt
     1861 attataatat ggaaaaatat ataagcctta tgtgtaaaaa gctagaagat ttttattatg
     1921 gttctgctat aaagagtgtt gttgattggt acatagaatc tcttggaagt gctaccatgg
     1981 aaaacaatat aatatctgta caaattgcac ttgaaacttt gtcatatgta attctagtgg
     2041 aacaaaataa gatacttaca gatgaagtat ttgactgtaa tttggcatct aaaaatataa
     2101 gactcttact tgatacatgt aagataccat atggtaaaca tgaattaaat atatttgata
     2161 atataataaa aaataaattt gatgatggag tggatttagt tatttattta agaaatagca
     2221 tagttcatcc aagtagaaag actcatagag ctgtgttaga ggttgaagat atttggaata
     2281 taatttcaat aggaacgaga tatattgaac ttgttttact ctttatttta ggttatagag
     2341 gagaatattc aaacagatta gtagaaagat gttatggtga agtggaagtt gtaccttgga
     2401 actagatttt tatatttatt ttaaaatata tattgacata tattgcctta tgctataaaa
     2461 tcaatatcat aataataatt cagaataaca ttaaatgcta agaaaagaaa tagtaaaagt
     2521 agttaatctt aaacagagag tttccgattg gtgagaggag caatagattt tactttgaac
     2581 acatctttga gcaggactct gaaagagtag aaatacttta ttaggagatt tccggaatcc
     2641 ttgcacccgt tatagtgcta gagtataata attttattgt acttaaagag gttagtgtcg
     2701 tgaggcatta atgaacagag gtggaaccac gagagttaac tcccgtcctc tagcagaagc
     2761 tagaggcggg agttttttaa tataattttt taagcgcgca gagaattata ttgcaataga
     2821 tacacttttt aaaaaataat caaaatattt taaaaattaa aaggcgaggg agaatattat
     2881 gaaaaatata ttaaaaaaag taggaatatt tacaattatg ttaggtttat taggtggagt
     2941 tgtaggttgt tcaaaaccag ataatgaaaa agataaggat gcttcaaagg aatctaaaaa
     3001 agaagtagtt gtagggtttg ataatacgtt tgtaccaatg ggatttttag atgaaaaggg
     3061 agatacagta ggatttgacg tagacttagc taaagaaaca tttaaaagac ttggaatgga
     3121 agttaagttt cagcctatag attggtcaat gaaagaaact gaattaaatg attcaaaaac
     3181 tgtagatgta ttatggaatg gatattctat aacagacgaa agaaagaaaa tagtatcata
     3241 tacagaacca tatttacaaa ataagcagat aatagttact ctaagtgact caaaaataaa
     3301 ttcaaaagct gatttaaaag ataaagaagt tggaacacaa caaggttcaa cagcacttga
     3361 tgcagtggaa aaggataaag attttatgaa tagcttaaaa ggtggagcac cagttcttta
     3421 tgatacttat gataaagcac ttagagattt agagataggt agaactagtg cagttgttgg
     3481 tgatgaagtt ttaattagat actatatggg acaaaagggt gaagacaaat acaaggtatt
     3541 aaaagacgat tttggattag aagattatgt tgtagcaact tctaaagaaa atccagaact
     3601 ttgtgagaaa ataaatgaaa cattaaaaga aatgaaaaaa gatggaactt ttgacaagat
     3661 ttatgataag tggtttaaat agaagataag ttagctgtta agaagggggc actaaaggca
     3721 tgttacaagg tttagagata gttatagcaa tgttttgtat aacattgata gtatcaattc
     3781 cattggggat tggagttgca tttttaagat tatcaaagaa taaattagta agtggtatta
     3841 cacaatgtta tatattgata atgagaggta cacctttact tttgcaaatg atagtcatat
     3901 tttacggatt acctctttta ggaattgtat ttgacagatt tacagctgga gtagtagctt
     3961 tcttcttaaa ttatgcagca tattttgcag aaatatttag aggtggaata cagtcaatcg
     4021 atagaggaca atatgaagct tcaaaagtac taggatttga taagtttact atgtataaga
     4081 gagtaatctt tccacaagtt tttaaaagaa tactagcacc tatttcaaat gaagtaatca
     4141 cattagtaaa agatacatct ttagtatata tattaggatt aaatgatatt ttaagaatat
     4201 ctcaaatagc aatgaacagg gaagcaagtc tgttaccatt atttgaggca ggagcgatat
     4261 atcttatatt tgtagctata cttactaagg gatttgaatt acttgaaaaa aaatattctt
     4321 attatagata aagttacaat attataggag gaggtttttt atatgttgaa aatcaaaaac
     4381 ttaaataaat catttaaaaa aaatagagta cttaaagata tttcttttga gttagaagaa
     4441 ggtcagattg gagttttact tggtaagtct ggtgcaggaa agaccacaat attaaggtgt
     4501 ataaatggtc ttgaagaatt tgatagtgga gagattataa tagataatga ggtaataaaa
     4561 aataagagag atatggcaaa aatacgagga aaaataggta tggtttttca aaatttcaac
     4621 ttatttcctc atatgacagt ccttgaaaat ataattgaat ccccagttaa tgtttttaaa
     4681 gttcctagaa aagaggctga agagagggct agagaacttt taagattagt tgatttagaa
     4741 gataaattaa attcttatcc atttgaattg tcaggaggac agcaacagag agttgctata
     4801 gctaggtcat gtgcattgat gccaaaagtc ctttgttttg atgaacctac ttctgcactt
     4861 gatattgata ctattcaaag agtagtcaat ataatgaaca gacttaaaga caaaggtatg
     4921 acaatattaa taattacaca tgatgttgtc ttctcaaata atgttgcaga taaaattata
     4981 agtataaaag atggtatagt agaaaatgtt caaataaaag aaaaaattgt ttagagtttt
     5041 ataaatataa actagggtag aattttataa tataaataaa gggcaagtaa aatcaaatat
     5101 actatgccct tttgttatct caaaatttaa tcttatattt tatgtttaag ttacattata
     5161 atttataata aaaataaaag gactatttaa atatataatg tgaataatgt ttaataattt
     5221 gaaatatgat aattttttat gttgaaagct tataatgatt atgatataaa ttggttatag
     5281 tatacaattt atgggtataa caataaaaaa ttagaaagga atttttataa aatgattaaa
     5341 ttatatggat atacaaaatg ctctactgtt aaaaaagcta aaaattggct taaggaaaat
     5401 aatttagaat ttgaagatat agatatggtt caaaaccctc ctagtaaaga ggagttaaaa
     5461 tcaatttata agactagtgg atatgatata aagaaatttt ttaatacaag tggtatgaaa
     5521 tatagagaat tgggtcttaa agatattgta aaaactgaat ctgatgataa acttttagaa
     5581 atattggtaa gtgatggtat gcttattaaa agaccattac ttttggatgg taaaaatgta
     5641 ttattgggtt ttaaagaaga tgtatggaaa agtactttgc taaaagaaga ttaggaagag
     5701 ataggtgtgt tgattaaaat tattaattaa tacacctata aatttatgta ttaaatctaa
     5761 tctttataaa tattaattag caaattcaaa tcaaaaaaat tcatatcaac aaacatatta
     5821 cttcttatta caaattatat catatgtttt atattaaaat attataatat tacaacgaat
     5881 tgttacaaaa ttacttatta ttaaccatga acatttgttt tgtgctatca tatttatata
     5941 agggaattgt cataaaaaat atcttgttca tacagtttta tatagaaaaa agaaatgaat
     6001 cttttcttta tataagtata ttttgtaaat tgcctctata tttatagttt aaataatagt
     6061 ggtattaaaa atctgacaac aaaaataaaa ctttcaaaaa attaaatcta aaaagaatag
     6121 gagataaata tgagtattga agatttatat gattttgaat gtgttccttt gaacaatgag
     6181 attgatattg atgttttatt tgaaaagatg atagaagatg gaaaagaaaa agaatataca
     6241 ccaattatag tcattgatga aaaagtaggg ctattaagaa aaaatataga tatgattact
     6301 aaagagtatg gttctcttga aaactataga gagtattgtt taacaaaata taatgaaatt
     6361 gatgtaaatc aattttttga ttttaggaag agtgagattt cagaattatt aaaggagcta
     6421 attgatttag aagatgattt ttatgattat aaacatttta gacccattat gaatatagat
     6481 atatttcatg aatataacaa agtttacata gctaaaattc caacaattaa accatatgaa
     6541 gtatttgcat acatacctat tggcggattt aatgattgtc ctagagatga agaacatata
     6601 gctattgcaa aatattggta tgaaaagcat ggaatctttc caatcgcaat tggatgtgat
     6661 acagttcaat attctgtaaa aaatttaact aaagatggta aaaaatttga tgacttatgt
     6721 attgaattag tattttattg tgaggatatt attatacaag ggtatgaaac cttaaaggct
     6781 cttaaggatg tattaaaacg ctctacaata gtgctttttt ggtgggattg atattctatc
     6841 atttagatag tattaaattg ttagtcacca agaatccact tgctttagtt atagggagtg
     6901 tgtcaaacta ggtttttata cacagtgtag catagtaata tgtttaaatt acaaaggtta
     6961 aaaaaatact ttaatatttt taatattata attaatagat ttaaggtaat tataatttat
     7021 aaaataaata ctttatatgt tataatcatt aagaatgtaa aaaaataata tatagtttta
     7081 tagttcatat ttttccagat atggaggtaa attttgtgaa ggaaaaagaa aaattagata
     7141 aattaaaaaa aatgttactt gaattaggca gtgttgtagt tgcttattct ggtggagtag
     7201 atagtaattt tttgcttaag gtcgcaaaag atacattagg tgaaaatgtt atagctgtga
     7261 ctattcatgc aatgatgcat tcaagtaggg aaattgaaga ggctaaacaa tatactcaaa
     7321 attttggtgt taaacatatc atattaaata tagaaaactt tgatttaaaa gaatttaaag
     7381 aaaatggtgt tgatagatgt tatcattgta aaaaatatat attctctaaa ataaaagagg
     7441 tagcaaaaga acataatata aagtacatag tagatggaac aaatatagat gatttaggag
     7501 actatagacc tgggcttaag gcattgagtg aattaggagt aattagtcca ttaaaagata
     7561 gtagtttaaa aaaagaagaa attaggtcgc tttcaaaaac attaggatta aatactttta
     7621 ataaaccatc atttgcatgt cttgcgagta ggatacctta tggagttgaa ataacagatg
     7681 aaaatttaag aatagtagaa aagagtgaag aatatctatc caatttaggt ttttctcaat
     7741 ttagagtgag aatgcatgga gatatagcta gaatagaagt tggacaggaa gagctaggta
     7801 aattttttga aaataacaat tttaataaag ttgatactaa attaaaaata tttggcttta
     7861 aatatgttac actagatatg tcaggatata aaatgggaag catgaattta aatgtgtaga
     7921 ttattttaaa aataaataag ttttatagtt aatataagga aacaggaggt tttatgaaaa
     7981 attatacgtt aatatcgcca tgtttttttg gaatggaaaa aatgctggct agagagatta
     8041 ctaatttagg atatgaaata ataaaaacag aagatggaag aataacatat aaaactgatg
     8101 agtttggaat agcaaagtca aacatgtggt tgagatgtgc tgaaagagta catcttaaaa
     8161 ttgctgaatt tgaggcaaaa agctttgatg aattatttga aaatacaaaa agaataaact
     8221 ggtctagata tataccttat ggagctcaat ttcctatatc aaaagcttcc tctataaaat
     8281 caaaattata cagtacacca gatgtacaag ccatagtaaa aaaggctata gtagaaagct
     8341 taaagaaaag ttatttggag gatggtttgc ttaaagaaga taaggagaaa taccctattt
     8401 ttgtatttat acacaaagat aaagtaacca tttctataga tacaacagga gatgctttgc
     8461 ataagagagg atatagagaa aaggcaaata aagctccaat aagagagact ttagctgctg
     8521 ggcttattta tttaactccc tggaaagcag gtagagtttt agtagaccct atgtgtggtt
     8581 ctggtactat attgattgaa gctgctatga ttggtataaa tatggcacct ggcttaaata
     8641 gagagtttat atcagaaaag tggagaactc ttgataagaa aatatggtgg gatgtaagaa
     8701 aagatgcttt taataagata gataatgaat ctaaatttaa gatttatgga tatgatatag
     8761 atgaagaatc tatagatata gctagagaaa atgctgaaat agcaggtgta gatgaatata
     8821 tagagtttaa tgtaggagat gcaactcaat ttaaaagtga agatgagttt ggatttataa
     8881 tcactaatcc tccttacgga gaaagattag aagataaaga cagtgttaaa cagttgtata
     8941 aggagcttgg ttatgcattt agaaaattaa aaaattggtc gtattatttg ataacttctt
     9001 atgaagactt tgaatatgag tttgggcaaa aagcagataa aaaaagaaaa ttgtataatg
     9061 gtatgttaaa aactaacttt ttccaatatc cgggtcctaa gcctcctaga aataataaat
     9121 aagttttaca tgatttattt gtaaaatata aaattttatt aaaataagta aaacattatg
     9181 atgccctgaa acgtctaatt attgtgagga gatttttgtg aacaaattta aagatttata
     9241 ggattaggag gagttaaatg aaagtgttaa agcttattac tgtagtagcc ctgatgatgt
     9301 tatttgttac tggatgtaat ataaaaactg aatctccaga acaattagcg aagatacctg
     9361 actatgatga tacaaaaaaa gtattgtatg atggtattga ccagttacta aaaccagatt
     9421 catctatgat attgccttct aatacaaaag aagtaggaaa aataaataag gtagatttaa
     9481 acagcgatgg aattgatgaa ttagtagtat ttgaacaaaa agaagattta agtaataatg
     9541 tgagtcaggt agggtttgta accttgagtt acaatggaga aaaatacgta cttggagacc
     9601 attttttaga aaatggcgaa tcaatagaat atgctaattt ttatgatttg gactcagatg
     9661 gatataagga agttatctta ttggttaaat ccaaagacaa aacgaatatg catatatata
     9721 aagtagtaga taatgaaata acaaaaatat atgacttgga tgcatcatgg cttcaaaata
     9781 aagaagattt taatgacatg aaagtaaaaa ttggatatat agatggagat gacaagttag
     9841 acatattgat gcttcatttg aataataaga ctaatgaaat gtttgcaagt gttgcaaatt
     9901 ttgatgggaa aatgaaaatc aaagattctg ttaagtttga aaatgtaaaa aacttaagtg
     9961 aattatatat aacattaggt aatgttgctt catctgttgg aaattcttct gcagctataa
    10021 aaggtattgt acttgatatt cctatattga aagataataa ctatattacg cagatacttt
    10081 atatgaaaga tgggaaaatt aacaaagctt ttagtgatta tgataaaact attactaaat
    10141 cttattatat acctgtagat gatattgaca aagataagat tatagaaata cctatagttg
    10201 caggcagtac aggtaataat aaaaatactt atagttctaa atcatctgca actataagtt
    10261 ggtatagatg gaatggaaaa gaaggtgctg actcaagttt aatatttaca agtcagatat
    10321 attataatta taaatataat tttaaattat ttattccaaa taatttattt gataaaatac
    10381 ttgttgaaca ggaatttgtt ggagaaaaag cagtatttaa attttactat tttgactatt
    10441 cagaccgtaa gaatttattt aatatagtag tagagagtaa aaataaactt gaagatagaa
    10501 aaaatacagg tactcaaaat tctattgtac ttcaagagag cgatgaatat actttcttac
    10561 tagttgtaaa caatgcaaat gaaatggata agctagatat gactatagac gcattaaaag
    10621 aatatttctc attaatatat ggttaatatt atactcaatt ggaggtttta ttttatgaag
    10681 gaaaagatac ttatactaga agatgaaatt ggtattagaa gttttgttag tataaactta
    10741 aaaagggaag gctatgaaat agttgaggca ggtactggaa gagaagctat agaaaaaatg
    10801 acaacagaaa aggatattac aatagctctt ttagatgtaa tgcttccaga tataagtggt
    10861 atagaagtat gtaaatttat aagggagaat tttgaccaag taggtataat aatgcttact
    10921 gctaaagctc aagaagatga taaaatagaa ggatttatct caggagcaga tgattacatc
    10981 ataaaaccat ttagtataaa agagctttta gtcagagttt ctgctcttct tagaagagta
    11041 gcaaaggatg attcaagtgt taagtcgagt gagatagttt ctccaccatt tatacttgat
    11101 atagacaaaa gaaaattatt taaaaatgga aaggaaattg agcttacacc aactgaattt
    11161 tcaatagtga aatatctaat atcaaatgct aagcagtcgt taagtagaga ccagatatta
    11221 gacgaagtat ggggaactaa ctatttatat gattttaaaa tagtagatgt aaatataaga
    11281 aggataagaa ataaaataga agatgaccca tcaaaaccaa aatatataca aactatttgg
    11341 ggttatggat actgttttag aaaggaagaa taatagtgtt acattttgaa ggtctgagaa
    11401 aaaaagtaat aaaaaactat tttataataa ttattataat ggttactctg tttgaaggat
    11461 tgttcatgtt ttacatacaa aactactatt atgattctgt caaacagcta cttgaatctg
    11521 aaataaaata tgctgatgaa tataatgcta ttacaatgga aactacaagt tttgagaaaa
    11581 aagtaaaaaa tatatttgat aaacaaccct taactaaaaa ttcagaattt ggaatttcaa
    11641 taatagataa agataaaaat attatattag accaatatgg ttttaaaagt aaagaaaaag
    11701 caaactatga agatgttaac aatgcattaa aagatataaa aactaaaaat ttaacacctt
    11761 atacttatag aataccagat acaggggagc atgttatgag tatctcctta ccacttaagg
    11821 taaataatat aatagaaggt gttgttagat atacagtatc attagatgct attgataatg
    11881 caatattaaa gcaagctaca tggcttatat tagcaggaat atttatactt attatagcta
    11941 tactaataag tcttaaattt gctgagactc ttataaaacc attacgagag ctaaagaaat
    12001 ttgcaaatga attggctgta ggaaattata atataaaatt agaaaagatg aagattgtag
    12061 atgatgaaat tggtgattta gcacagacat ttgaacacat ggcacatgag attgacaaaa
    12121 gtgaaaaatt aaaagaggaa tttatatcat ctgtatctca tgaactaagg actcctctta
    12181 catctataaa ggggtggagt gaaaccttag gttatgaaag tataacaaga gaagaactag
    12241 acttaggttt aggcattata caagatgaaa cagaaagact tataaaacta gttgaagaat
    12301 tattagactt ttcaaggctt tcttcagaca ggataaaact tcatgttgat atagttgatg
    12361 ttgaaggttt aattgtaggt gttgtaaatc aacttaaagt taaagcagct gaaaaagata
    12421 tatcactttt atttgaattt gaaaatgaat ttatagagaa cattcaagga gataaaaata
    12481 gattaagaca ggttctcata aatttaatac aaaattcatt taaatttacg agtcaaggtg
    12541 gttacataaa agttgtagct tctcaagatg aagagataac aaccatatca gtagaggata
    12601 atggttcagg tatagaaaag caaaatttaa ataaagtgtt agataaattt ttccaagaag
    12661 attataataa ggctggtagt ggattaggac tagcaattag taatgaaatt gtaaaacttc
    12721 atggtggaag aatgaaaata gaaagtgaaa agaatgttgg aacaaaaata acttttaata
    12781 taaaaaataa atttgctaaa caagcataaa ttcttagaaa gaggattaag atgagaaaca
    12841 taaaaatgat agtagcatac gatggttcaa gatataaagg atatcaaaaa ttaggtgaca
    12901 ataatatgac tattcaagaa aaacttgaaa atgttttaag taaaatgact aatgaaacag
    12961 tggagataat aggttctgga agaactgata tgggagctca tgcaagagga caagtggtaa
    13021 attttagaac taattgtatg gattctttgg ataaaattca aaaatattta tatgaatatt
    13081 taccagaaga tatagttgta aaaactgttg aagaagttga tgaaagattt cattcaagat
    13141 acaatgtaaa atcaaagact tatatgtata aaattgacaa taataagtat cataatcctt
    13201 ttataagaaa atatgctact catgtaagta aaaaattaga tttagataga atgagaaaag
    13261 caagtgagta tttagtagga gaacatgatt ttactagttt tgcttcttct aaatctaaga
    13321 aaaaatcaaa tgtaagagaa atttattcta taaatataaa agaagatgat aatgttatag
    13381 aaatatatgt agaaggaaat ggatttttgt ataacatggt aaggataata gtaggtgctt
    13441 tgatagatgt tggtctaaaa agaagagctc cacaagatat taagtatatg cttgaatcaa
    13501 aagatagatg tcaatcatca gatactgcac cagctaaagg actatgtctt tggaaagtaa
    13561 catattaatc aattatataa aactctcttt aaaataaaaa taaaataaat tatatagttg
    13621 acataaatgg aaaaataaac tattatatat tttataagag agatataaaa ctttatattt
    13681 aaatcctgtg agaaagagag taacttctat gtgttttata gagagctgag gatggtggaa
    13741 gcttggcaaa taagtattag tgaaaagaac tttggaggat actaattgaa tttagtatgt
    13801 tagtaggtaa tctcgcctta agagaaagag tggataagtt tatcttatca attagagtgg
    13861 taacgcggat atatttcgtc tcttggtttt tccaagaggc gttttttaat atatttaatt
    13921 attagtgttt atctggtatg ttgaacattt actaattgat tagctaagtt tatcaataag
    13981 agtggtaccg cggatatttc gcctcttagt attttaaaat actaaggggt ttttttagta
    14041 ttaaaatgat tttatgtgag tctaaattct cagagtcatt caatattaag aatttctata
    14101 aaataaaaat tataatttaa gattgggagg aattaaaaat gaaggaaaaa gtagtattag
    14161 catattcagg aggacttgac acatcaatca taataccttg gttaaaggaa aattatgaag
    14221 atatagatgt tatagctgtt tgtggaaatg taggtcagga agataaaatg gaagatgtat
    14281 atgaaaaagc tttacaaagt ggagcatcaa aggcatatgt ggatgatata agtgaagaat
    14341 ttgtaactga aactatattt aaagccgtaa aagcagaggc taaatatgaa ggaaaatact
    14401 tattaggaac ttctttagca agacctataa tagctaaaaa attagttgaa gttgctcata
    14461 aagaaggtgc aaaatatata tgccatggat gtacaggaaa aggtaatgac caagttagat
    14521 ttgaagctac aattgcagct cttgacccaa ctataaaagt tattgcacct tggagaattt
    14581 gggatataaa atcaagagaa gatgctatag actatgctga aaaacataat ataaaggtaa
    14641 cagcaactaa agctaaaata tattcagtag atgccaacct ttggcatgtt tcaacagaag
    14701 gtggagatat agagcaccta gaaaatgaac ataaaaaaga tgtttataaa caatgtgtag
    14761 acccagaaga tgcatgtgat gtagctgaat atgtagaagt ttactttgaa aaaggtgtac
    14821 ctaaaaaggt aaatggcgaa gaattatctc cagttgcact gattcataag ttaaatgagt
    14881 tgggttgtaa acatggaatt ggtgttatag atatagttga gaatagactt gttggtatga
    14941 aatcaagagg tatatatgag actccaggag gaacaatcct ttatgaagca cataatattt
    15001 tagaaagtgc tactttagac aaagatacat tacatttcaa acaaatggta tcttataaat
    15061 acggagaact tatttacaat ggactatggt attgtaaatt aagagaatct atagatgcat
    15121 ttatggaaca aactcaagat aatgttacag gaacagtaaa agttaaactt tacaaaggaa
    15181 atataaaacc tgctggaata tttactgaaa atgctcttta tgatgaagga atatcttctt
    15241 ttggaaatag tgaactttat gaccacaaag atgcagaagg atttattaat ttattcactt
    15301 taccattaaa aatcagagct atgaaagctg gaaaataata aaataaatta tttaagagtg
    15361 tataaaaacg atttttaggt ttttatatgc tcttttttta tttgaattta ttaaattata
    15421 cagtaaatag tataaaaaaa tatattatag ttccaaatgg cttaataatg tgaaaatata
    15481 agacctaaaa tataaatttt ataaatacaa tagttattga ctctctcatt gtaagagggt
    15541 ttatactgaa tctaatgaaa atttatagtg aggagataga ttatatgaga aaaataattg
    15601 aaattaaaca tcattcacat gattatgagt gtatgtggaa tggtatagaa gatttatata
    15661 taaataaaac cggtgaatac cttccaaacg attttttctt tttactgtca ggttttggtt
    15721 cattttgtta tatgaaaaca aataaagctg atttgaaaag aatggtagct ttgggagatg
    15781 gaaggacaaa acagatgtat aaatttttag caccaattgt tggatttgaa tacaaacatc
    15841 atgagtttaa aaagtatgag caagcattaa agaaagcaaa atctgaaatt gatgatggat
    15901 atccagttat tttaggagct ttagatatgt attatctacc atattataag aagctatatc
    15961 ataaagaaca tattcctttt cattatatat taatggtagg atatgatgat gaaggaatat
    16021 atctgtatga ttgtggtaga acagaattat tacatctttc ttatgataaa ttacgtgaaa
    16081 gcatgaattg caattcccct ggattaagca aagaaaatac tatttgcaca ataagaatga
    16141 atacttcaaa gaataaatat aacattgcta gagaagctat tgctataaag cgtgatatgt
    16201 ttttaaatcc acctaaagga tttttgggat ataaaggttt tgaaaagttt attaaagagt
    16261 tgccacagtg gaacactgaa ttggataagg atgattatga taagattttg accaatatgg
    16321 ttatgttttt tggaacagta ccaacaattc caaatgcaat caaaggaatt aaagaaccag
    16381 atactgttca atttaaaggt ggatttgata aaatgagtag aatgctcaac agtattgggg
    16441 aggaatacaa aaacaactct tttatacaag tagctgatat ttttgataaa actgctatta
    16501 taattgagaa aatatccaat attataatag attacttaac tcaaacatgt gatgatacag
    16561 aacaacttcc gttattgttc tctgaggtct tagagcatat gaaaacagga tatttaatct
    16621 tggatgtata aaaaatatat tgttgaccct aacattacgt aatagtgtaa tatgaaaata
    16681 tagcttaaat atattagtag gtattgatgg ctgtaaaatt aaaatttgag gtgtggatat
    16741 gagaacagtg aaacaagttt ctgatttaac tggaataagt gtgagggcat tacattatta
    16801 tgatgaaata ggtcttttaa aaccaaataa aattacagat gcaggatata gactttatga
    16861 tgatgaatct attaaaactc ttcaacaaat tttatttttt aaggagattg atattccttt
    16921 aagagaggtt aaggaaataa tgtcaagcca atattttgat aaggttgaag ctctaaaaaa
    16981 tcaaaaaaaa ttacttattt taaaacgaaa gagactggat gaattgatag agcttataaa
    17041 tcaaactcta agaggagaag gaaatattga ttttaaagag tttgatatga gcgagtattt
    17101 taatgtttta gaagaattta aaagagagca taggaataag gtaattaaaa tttatggtag
    17161 tgtagaaaaa tacaatgaat atatagaaag agttaagtct aatgaagaga aaattgctaa
    17221 aatggctata aaacaatatg gaactattga gaagtttgct aaagctataa aaacaaattt
    17281 tagcagtgat atattgaatt taggagaaaa atttgataga tataaaaatg attgtttaaa
    17341 agaaaaacat ccaaaattaa aagaattata tagaaagctt gtagaagatt taagtaggaa
    17401 tccttcttca acagaccttc aagaaatagc aaaagcgata acagatatat ctaaaaaaga
    17461 ttatgaaatt ttctctatgg atactggtga tgacaactgg tattatatgg ttcaaaatta
    17521 tctagtaaat cctatgtgga tagaagaagt agataaaaag tatggaagtg gagcaggaaa
    17581 gtttattgga caagtactaa agacttatct aagagataga aaaccaaaga taaatacatt
    17641 gtatgaaaaa cttgtagaag atttgagtag agattgttct tcaagggaag ttcaaagcat
    17701 tgttgaagaa atagacaatg aaatgaaaag aagtaatgaa ttttataaaa tagacaatgg
    17761 tgaaagatac tttgattata tgtcagagct ttatttacaa gattctaact atataaaagt
    17821 aactgataag aattatggtg atggtgcatc taagtttatt ggagaagctt ttaagattta
    17881 ttttgataat aataattgtt gaaaatttgg aagtaaaaaa tatgtaagtt ttataaaagc
    17941 acaaaaataa ggatgtagat tagctccatc tatatcctta tttaaagtta ttaaaatata
    18001 tagtttacat caaacttaac actttcaata atatttccca aatatgtttt tatactttca
    18061 acttcttcta aagattttaa tttattttca gtataaattg ctttgtccag aatattaaac
    18121 cttaatccta tttcatcaaa gtactcatga tttaaaactt tgtaccaatg gtttttttct
    18181 ttattccaac tcgtagatat tgaatcagct tctttttgag ctttttccca atcttcattt
    18241 tctatagatt tttctaaaac agatatattg tctttatatc cttctgtaaa atcatatagt
    18301 ttattgttaa cgtaaaatcc aaataataca aataagatag tccatagtat aacaaatgtt
    18361 gtagatttca atattaatca ccctttttat tttgaataaa tattttgtca ttttcatcta
    18421 aaacacaaat aagaacatct ttaaaagttt ctatgtgatt agattttaaa atacccatta
    18481 accagttagt atctttttga gctatgtcta gatttttatt tattattcta ccatccagta
    18541 taagtgggat aggtaaatga ttaaaagctc ttggaggagt actattgtag cttgatgctg
    18601 gtacaacact tagattacca tcagtctcca aaatagcata ttgtacatct tttaggttaa
    18661 agtatccttg aatcctaagt tgttctaata attcatctat actaattctt tcttttttaa
    18721 gctctttata tacaattttt cctttatcta ttaatacaga aggtttacct gataaaaatg
    18781 aacttaattt tctgcttttt aatgataaga atgaaattag agtctgcatg attacaagtc
    18841 cagatattgc tgcaacacca tttatgattg gtatattatt attttccatt gggacagatg
    18901 caacttctgc aattaatagt gttataacta aatcaaaaca gttcatttca gctatttcac
    18961 cttttcccat gactctaagt gcaattagca ctgttatgta taatataata cttcttatta
    19021 atactactgt catatctatc aattactcct ttaaaaaata tttacactta gttagtatat
    19081 cctatataaa aaatttaatt caaagtataa aaaaccttaa ataattggat tcaactattt
    19141 aaggtttttt tgataatttt a
//
LOCUS       DAGSRM010000075        19147 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.075, whole genome shotgun sequence.
ACCESSION   DAGSRM010000075 DAGSRM010000000
VERSION     DAGSRM010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19147
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.075"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            144..1142
                     /locus_tag="MJE33_002709"
     CDS             144..1142
                     /locus_tag="MJE33_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HBY9648637.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENVVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLINTGDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            1504..2031
                     /locus_tag="MJE33_002710"
     CDS             1504..2031
                     /locus_tag="MJE33_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HBY9648638.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASVVAGIVGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            2058..2654
                     /locus_tag="MJE33_002711"
     CDS             2058..2654
                     /locus_tag="MJE33_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HBY9648639.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDDYDEIVFCGYGEPLMRINELIEIAKFIKEKSSIKIRINTNG
                     LSDLIHNKKTAILLKNVIDAVSISLNAPNKEAYNRVTQPKFGEKSFDYMLDFARDCKK
                     YIKEVAFSVVDEISPEEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(2719..4302)
                     /gene="asnB"
                     /locus_tag="MJE33_002712"
     CDS             complement(2719..4302)
                     /gene="asnB"
                     /locus_tag="MJE33_002712"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBY9648640.1"
                     /translation="MINLEEKYLTRKDTYMIKILEYNGHIRNWKVLCEELGIDSSLCR
                     DERERAILVEAYKTWGYDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYETEN
                     GKLLYGSSIRKIMEQPGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
                     NKTVCIGRYWKPEFHPDHSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSS
                     YILAMSDIEKTGSCGYEEERFDESKLAQQTANILGCKNSRYIITPEEYFASVPYVMYH
                     MEQPTGDASAIVFAIGCKAAAENTNICYSGEGADEFFGGYNIYHKAECYGENLKTFYI
                     GNTNIMNEGEKRKILKKYNPDVLPIEVVKGIYEETEELDTLTNMSNVDIQIWLEGDIY
                     FNIDKMSTAVGLEVRMPLTDTRIFDIASRIPSEYKVKGKENKIAFRTAASKTLPEEIA
                     FRNKLGFIVPIRIWMADDRYNKDIRDKFNSEIADKFFNIDEINEIFNDYISGNSDNWR
                     KIWMIYTFLVWYEIYFVKC"
     gene            complement(4868..5704)
                     /locus_tag="MJE33_002713"
     CDS             complement(4868..5704)
                     /locus_tag="MJE33_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBY9648641.1"
                     /translation="MNYIIKKVLNSSVVLVHDAKYNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKQFLELMDDIPVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKGIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAMASDGNSYDTIKYAKVIGEIINIFVFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSGDDLLFNQMKRSNPKEMLIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     KQ"
     gene            complement(5728..7194)
                     /locus_tag="MJE33_002714"
     CDS             complement(5728..7194)
                     /locus_tag="MJE33_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBY9648642.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGGKGWSVSDVAKAHFDADV
                     KDYKSNNEITTKDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDKEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVINMFVRYVETICERYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEEVI
                     FQAMHHQFVASAIATKICHEIIPNSKVGCMLTKLTYYPYTCRPEDVLATQQKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIVKEEHDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDITPGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRINYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIENNSIDFK"
     gene            complement(7197..9059)
                     /locus_tag="MJE33_002715"
     CDS             complement(7197..9059)
                     /locus_tag="MJE33_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBY9648643.1"
                     /translation="MNYKKTAIDIIEQVGGSKNILALEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVNGAQFQVVIGNSVVDVYDEILKVCPLDTNDSTVKTEEKINLGTRFIE
                     FIINIFQPLIFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLVAISDATFYFLPLMVA
                     VTTANVLKCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPNIAYNAQVFPAILCVS
                     FLATMEKIFNKYSPKPIRTFFVPMMSLAITVPITLTILGPLGYNVGTIFTTIILFLYN
                     KMGFLVVGLLAAFLPFMVATGMHKALIPYSINTIGKLGYEALYMTSSLAHNISESGAC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAMLGVVFSSAISGTFLG
                     LFAVKGFVIVGPGLASMSMFIDPNNGNNLIFASIGFVIAILGSFIITLIIWKEDTNTV
                     ESSIGDVVEEELEEEKEVNILVTPVEGKVIDLSKVNDELFASKTLGDGVAIIPTNGNL
                     YAPCDSEVVMLFETKHAIGLRTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEGD
                     LLIQFDLDKISHANLDSTVMIVNNNGADYAYKVLNQSYGNVKKGSILFDVKGGI"
     gene            complement(9306..10169)
                     /locus_tag="MJE33_002716"
     CDS             complement(9306..10169)
                     /locus_tag="MJE33_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBY9648644.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDRVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFLKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKIICEIFTNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            complement(10224..11156)
                     /locus_tag="MJE33_002717"
     CDS             complement(10224..11156)
                     /locus_tag="MJE33_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HBY9648645.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKDVISHNYKYYLPILNRHFRDYDFAGVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGNSNPRYFKNLINAIEDIKNENLLDEFTTLTEATHWSGTTYGEES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEPLKTLLCVGGVHFEK
                     SFSDIIKNKEYNISIGHVLPNQWIVSGMYDDESGFEKLEKCINSIEGGIDCIVFHDKL
                     KGTYKEQCRKLGEKLNVPVFKHKILKNPKDLPIW"
     gene            complement(11242..11793)
                     /locus_tag="MJE33_002718"
     CDS             complement(11242..11793)
                     /locus_tag="MJE33_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY9648646.1"
                     /translation="MQSLNEIIAENLKKIRKEKHLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDIDHFIEDNGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYIVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYI"
     gene            complement(12022..12513)
                     /locus_tag="MJE33_002719"
     CDS             complement(12022..12513)
                     /locus_tag="MJE33_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648647.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMDDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
     gene            complement(12541..13101)
                     /locus_tag="MJE33_002720"
     CDS             complement(12541..13101)
                     /locus_tag="MJE33_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBY9648648.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVSNWIDIEKIIKQGI"
     gene            complement(13253..14410)
                     /locus_tag="MJE33_002721"
     CDS             complement(13253..14410)
                     /locus_tag="MJE33_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBY9648649.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRDFFIELEVIFKSGRKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVVIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDSILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKTKTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            complement(14447..15388)
                     /locus_tag="MJE33_002722"
     CDS             complement(14447..15388)
                     /locus_tag="MJE33_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648650.1"
                     /translation="MRWILVFIFLYLIPLVVIFRNYKNFKRSCIYSSIYVVLATTIMI
                     SNIYMSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLKQDNKKNEEISNNKNL
                     NNKQDRNIQNKESNEEGSNDIKEKNNSDSNVSVNNKSEKQEDNTKENNKYTSKEKNSE
                     DIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSIKKDVTNARNK
                     CKEVVKEYENMQIPELSKEKYTETLSNARDDVKKAYELREKSMESALKLINTKSPKYI
                     GKITEYLDLSDKQIESFKNRLNDLKEEINNSETNNIN"
     gene            complement(15475..16167)
                     /locus_tag="MJE33_002723"
     CDS             complement(15475..16167)
                     /locus_tag="MJE33_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBY9648651.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVKEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYEDMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            complement(16276..17088)
                     /locus_tag="MJE33_002724"
     CDS             complement(16276..17088)
                     /locus_tag="MJE33_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBY9648652.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            complement(17139..18050)
                     /locus_tag="MJE33_002725"
     CDS             complement(17139..18050)
                     /locus_tag="MJE33_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBY9648653.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYVFEDNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVCIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKSIEAGKIELNNEMFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLYQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            complement(18109..18840)
                     /locus_tag="MJE33_002726"
     CDS             complement(18109..18840)
                     /locus_tag="MJE33_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBY9648654.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFDKLNDVYKSLPVGNCSGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKILDYYFEEYTRKNPCPFKDTNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSKRLSFFDELMILDSNIYSSGNIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEYKIRKRTIDRIKRIVLV"
     gene            18929..>19147
                     /locus_tag="MJE33_002727"
     CDS             18929..>19147
                     /locus_tag="MJE33_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648655.1"
                     /translation="MNRYTKIINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQG
                     DCEVLVVFEDTGKEILIDDFSPEEDIKKY"
BASE COUNT         6443 a         3287 c         2068 g         7349 t
ORIGIN      
        1 tcaaaataac acagggaaaa caatttcaaa actaaatttt taaccttaaa aattttgtat
       61 aaaaaatata accttatatg ctacatatta tttctaaatg aaaaattacc ataataattt
      121 aataattaca ggagggtatt gatatgaaaa tactagtatt tggagcacgc gattatgaag
      181 aaccagtaat aaaaaaatgg tctgaagaac ataaggatgt tcaagtggat atttatcctg
      241 aaaacatgac tgaagaaaat gtagttaaag ctaaagggta tgatggtata tctatacaac
      301 aaactaacta tatagataat ccttatattt atgaaacttt aaaagatgct ggggttaaag
      361 ttatagcttc aagaactgca ggggttgaca tgatacattt tgatttagtt aatgaaaatg
      421 gacttatcgt tacaaacgtt ccttcttatt cacctaatgc aatagctgaa ttagctgtta
      481 ctcaagctat gaacctttta agaaagactc ctctagtaaa gaaaaaagtc tgtgaaggtg
      541 attaccgttg gatagctgaa cttcttggaa cagaagttag atctattaca gttggtgtta
      601 taggtacagg aaaaataggt gctacttctg caaaattatt caaaggccta ggagctaatg
      661 taattgcatt tgaccaatat ccaaatagtg atttaaatga tatattaact tacaaagatt
      721 ctttagaaga ccttctaaaa gaagctgacc ttataacatt acatactcct ttacttgaag
      781 gaacaaaaca tatgataaat aaagatactc tagctataat gaaggatgga gcttacatag
      841 taaatactgg ccgtggtggt ttaattaata caggggattt aatagaagca ctagagtcag
      901 gaaaaattag agctgctgcc cttgatacat ttgaaactga aggattgttc ttaaacaaaa
      961 aaatgaatcc tggagaatta actgacccag aaataaataa acttctttct atggaacaag
     1021 ttatattcac tcatcacctt ggtttcttca ctagtacagc gattgaaaat atagtttatt
     1081 ctagtttaag tagtgctgta gaagttataa aaacaggaac tgctactaat agagtaaatt
     1141 agtattttat aaatatattt tttcattaat tagctattat ctaacaaaat agcctttaat
     1201 ttcccaaata taagagagtc agctcagtat atattgagta gactctctta tattattttt
     1261 tatattaaac ttgtactctt cttattcata ccaaattaaa tttatattaa cttattctaa
     1321 ctactcctac tctatacatt ttacctattt ttacttttct aattatatac atttgaatgg
     1381 gtacaatctg tattttacaa aaaaaacacc taacaaacta tgaaaattgt atgattaata
     1441 ttaatatatt tataagaaaa cataacaatt atgttagctc tggattttta gggagggagt
     1501 atattggaaa aaatgaataa agttacttct aaaaaaacta cctatattgt tacttcagca
     1561 ttatttgcat ccataatatg cctgacaata gcatatatat tacatattcc agttggtgga
     1621 aacaatggat atgttcatat aggagatgct tttatttatc tagctgcaac tattttgcca
     1681 acaaactatg ctatagctgc ttctgcaatt ggtgctggtc tagctgattt atctacaggt
     1741 gctgctatct gggtaatacc aactataatc ataaaaccaa tacttgtatt atttttcact
     1801 tctaaaagtg acaagataat aaataagaga aatatagttg cttcggttgt agcaggtata
     1861 gtaggattag ttttatacat gtttgctgaa ggtataatta ttggaagttt tacaagtgca
     1921 tttgtaatga gcttacttgg cttattacag ccaataggaa gttttattgt ttttataatc
     1981 ttaggtatgg cacttgataa acttgatttt aaaaaacgtt attttaatta agaatttaaa
     2041 tttatggagg gaaaattatg aatatcttat atacaattga aaatagtatc tatgtaaata
     2101 taactaatac atgtccatgt agttgtgttt tttgtataag aaacgaaaaa gatgaagttg
     2161 caaatagtgg tagtttatgg ttggagcatg agccaagtgt agatgaagta aaagaagcct
     2221 tcaataaata caatttagac gactatgatg aaatcgtatt ttgtggatat ggcgaaccat
     2281 taatgagaat aaatgaattg atagagatag caaagtttat aaaagaaaaa agtagcataa
     2341 agataagaat aaatacaaat ggtctaagtg atttaataca taacaaaaaa acagctattc
     2401 tgttaaaaaa tgttatagat gcagtatcaa taagtcttaa tgccccaaat aaagaagctt
     2461 ataatagagt tactcaacca aagtttggag aaaaatcatt tgattatatg ttagattttg
     2521 caagagattg caaaaaatat ataaaagaag tagctttttc agtagtagat gaaatatctc
     2581 cagaagaaat agaagaatct aaacaactgg caaaaaaact tgatatacca ttaagagtta
     2641 gacataagaa ttagcaagta aaattataaa acattaagaa tcacctgaat ggtatttcag
     2701 gtgattcttc ctcttgtatt aacattttac aaaatagatt tcataccaca ctaggaatgt
     2761 gtaaatcatc caaatctttc tccaattatc agagtttcca ctaatgtagt cattaaaaat
     2821 ctcattaatc tcatctatgt taaagaattt atctgctatc tcactattga atttatctcg
     2881 gatatcctta ttatatcggt catcagccat ccatatacga attggcacaa taaatcctaa
     2941 tttatttcta aaagcaatct cttctggtaa tgtttttgat gcagctgtac gaaaggcaat
     3001 tttattctct tttcctttca ctttatattc tgatggtatt ctggatgcaa tatcaaatat
     3061 tcttgtatct gtaagaggca ttctgacttc caatcctact gcagtactca ttttatctat
     3121 attaaaataa atatctcctt ccaaccatat ttgaatatct acatttgaca tgttagtaag
     3181 tgtatctagt tcttctgttt cttcataaat tccttttacc acttcaatag gaagcacatc
     3241 aggattgtat ttcttaagaa tttttctttt ctcaccctca ttcataatgt ttgtatttcc
     3301 aatataaaat gtcttcaaat tctctccata acactctgcc ttatggtata tattatatcc
     3361 accaaaaaac tcatctgctc cttctccaga gtaacagata tttgtgtttt cagctgctgc
     3421 cttgcatcca atagcaaaaa cgatagcaga tgcatctcca gttggttgct ccatatggta
     3481 cattacataa ggtactgacg caaaatactc ttcaggtgta attatatatc tcgaattttt
     3541 acacccaaga atattagctg tctgttgtgc taattttgat tcatcaaaac gctcttcctc
     3601 ataaccacat gaacctgttt tttctatatc tgacattgcc aatatataag atgaatcaac
     3661 acctcctgat aggaataact ctgcagtttc attctctgat tttacctctg gcataatttt
     3721 ctgaagtgtt gtatgaattt catccgtcca ttcctcaata gacttgctgt ggtcaggatg
     3781 aaattcaggt ttccaataac gaccaataca tactgtttta ttctgataga caaggtaatg
     3841 tcctggcata agcttcttta ctccacggaa gaatgtattt tcacctgcta catatgtgaa
     3901 agacatataa atctgtaaca tgtcttcatt taactctttc acaaatcctg gttgctccat
     3961 aatcttacga atagatgaac catagagaag cttcccattt tcagtctcat aataatagaa
     4021 aggttttacc ccaaactggt ctcttaggca gaagagtttc ttttccacag tatcccataa
     4081 tgcaaaagca aacattccat gcatgtgatt tgccatatca tatccccatg tcttatatgc
     4141 ttccactaaa attgctcgtt cacgctcatc tctacataat gaagaatcga ttccaagttc
     4201 ttcacaaaga actttccagt ttcgaatatg cccattgtat tctagaattt ttatcatata
     4261 tgtgtctttt cgtgttaaat acttttcttc taaatttatc atatgtgtat cctttcgtat
     4321 taaaacattt aacttctaaa tttattaaat gtttatatat atttattcac ttataatcac
     4381 tgaaaaatcg acttttttca aagtaaattc tttttgaatt agtactaatc tgctctttat
     4441 taatggcata aatatgatta tcaagtatta tcagtgttat aacattgaat tttttattct
     4501 tcataaatat tgcccccttt atattaccaa ctaaatataa tatatcttat gttacttaca
     4561 tattctttaa gaaattctga agattttata aagttttttg tattataatt aattaaaaat
     4621 aaaattctag ttataaaaca tattttccat taaacaaaaa aatagttaaa ataaattcat
     4681 aaccacataa acttatttta actatttgta tattttaatt ataaatatta acttaaattt
     4741 ttaacaggat acactcataa aataaacata atgaacaaca aaataatatg tctcagtttc
     4801 tttatatact ttaccttctt tcaattttct accatgtaga cgtataagat aatattagta
     4861 attcactcta ttgcttaact cgtgcgatat gtacaaccaa gaaagctatt tcctcatttg
     4921 ttaacttttt tccatatttc ttttctaaaa aatctctaac ttttaatgca attaacattt
     4981 cttttggatt tgaccttttc atttgattaa ataataaatc atcaccactg cttaacatac
     5041 tatctgaaaa aaatctttcc acaaaaaatt ttatatgtgt tataaatctc atataatgca
     5101 tactcttttt atctaaggtt ctattcaaag aaaaaacaaa aatattaatt atttctccaa
     5161 ttactttggc atacttaatc gtatcatatg aatttccatc tgaagccata gcatttgcaa
     5221 aatggaaagc aatgttagct gcttcttcct ctggtaattg aatgcccaat ctctcctcta
     5281 ccatataaat tgcaaccatt ccaaccttaa actcatttgg ataataattt tttatttccc
     5341 aaaatactcg atttgtaatt tttattcctt tacgcatcct ttcaattgca aagttaaaat
     5401 ggtctgctaa tattaagtat aagtttttat taaaagatga gttcaataac ttttctgcct
     5461 ctactattat ttcctgtgtt atctttaaaa tgtcgacagg aatatcatcc attaattcta
     5521 aaaattgttt tgattttgta ttttcaacag ggacaaacat ttgattatca ttggaaccac
     5581 aaatatattc tcctgttttt ttaccatatc caattccttt ccctagcaaa ataaattcat
     5641 tatactttgc atcatgaact aaaacaacgc tcgagttcaa aactttcttt attatgtagt
     5701 tcattttatt tgctcacctt ctattggtca tttaaaatct atactattat tttcaattac
     5761 ctttttatac caataaaaac tatctttctt atatcgatta tatgtcccct ttccatagtc
     5821 atcagcatcc acataaatat atccataacg ttttgacatt tgtttcgttg attcactgac
     5881 caaatcaata caaccccatg aagtatatcc ccaaagttca acgccatctt cgataatcgc
     5941 atctaacata catttaaagt gttcttttaa ataattaatt ctatattggt catgaacttt
     6001 tttatcttca gttaaaatat ctttagctcc caatccattt tcaactataa ataaaggctt
     6061 tctgtatcta tcatataatt caatcaaaga tatacgtaga cctattgaat caatttgcca
     6121 accccattca ctcatttcta aataaggatt ttttacagct aataacgtat tcccaggtgt
     6181 aatatctaat ccagttgtat cagctgcctc acaagaagac atatagtaag agaatgaaat
     6241 aaaatctact ggatactttt tcataatttc atcatcatgt tcttctttta ctatttgaat
     6301 gttattattt ttaaaatacg acagtaaata tactggatac tcaccaaaaa cctgcgtatc
     6361 actatatgca taaatacttc tcattttttg ttgagttgcc aaaacatctt ctggtctaca
     6421 tgtatatgga taatacgtta attttgttaa catacaccct acttttgaat ttggaataat
     6481 ctcatggcaa atttttgttg caatggcact agctacaaat tgatgatgca tagcttgaaa
     6541 aataacttcc tcaaagtttt tatccttaaa acggtctcga actaaaccac cagtagtgta
     6601 cggatgacga atcatagaat caacttcatt aaatgtaagc caatatttca ctttgttttt
     6661 atatctttca cagatagttt ctacatacct aacaaacata ttgataactt gtcgtgaata
     6721 ccatccatca tagtttaaaa cgatatttaa tggtggttca taatgtgaca tagtaacaag
     6781 tggttcaatt ccataactta tcaactcatc aaatacatca tcataaaact gtaaaccttc
     6841 ttcatttggc tctttatcat ctccatttgg aaaaatacga gaccaagcaa tactcattcg
     6901 atatgtctta aatcccattt ctgccattag agcaatatct tctttatagt gatgataaaa
     6961 atcactacca tgacgttttg gataatttac ttcatcttca ggatgtgcta atccttcttc
     7021 tatatctttt gttgtgattt cattattaga tttataatct ttcacatctg catcaaagtg
     7081 tgcctttgct acatctgata cagaccaccc tttcccacct aaattccaac caccttcaaa
     7141 ttgattagca gctgtagctc ctccccaaag aaatccttct ggaaattttc tcatatttat
     7201 attcctccct ttacatcaaa caagatactt ccttttttta cattgccata agactggttt
     7261 aacactttat aagcataatc tgctccatta ttgtttacaa tcataacagt tgaatctaaa
     7321 ttagcatgtg agattttgtc taaatcaaat tgaattaata aatctccttc ttttacatta
     7381 tcgcctgtct taacaaatgt tttaaatcca tctccattca tacttacagt atttattcca
     7441 atatgaatca aaatctcagc cccatttttt gttcttaaac caattgcatg ttttgtttca
     7501 aataacatca caacttctga atcacaaggt gcgtataaat ttccattggt tggtataatt
     7561 gcaactccat ctcccaaagt cttacttgca aatagttcat cattaacctt acttaaatct
     7621 attacttttc cttctactgg agttactaag atatttactt ctttttcttc ttctagctct
     7681 tcttcaacaa catctccaat tgatgactct actgtatttg tatcttcctt ccaaataatc
     7741 agtgtaataa taaacgagcc taatattgca ataacaaaac caatactcgc aaaaatcaaa
     7801 ttgtttccat tgtttgggtc aataaacata ctcatactgg caagccctgg tccaacaatt
     7861 acaaatcctt ttactgcaaa taatcctaaa aaggtaccag aaattgcact tgagaataca
     7921 acacctaaca ttgctctttt atgttgtaag gtaataccat ataaagctgg ctctgtaatt
     7981 cccatcaagg cagaaattcc tgcagataat gctgtttgtt ttaatgtttc atcttttgtt
     8041 cgcaaggcta ctgcaaaaca agcaccactt tcagaaatat tgtgggctaa tgaagatgtc
     8101 atatataaag cttcataacc caactttcca attgtattaa ttgaataagg tattagtgct
     8161 ttgtgcattc ctgttgcaac cataaatgga agaaaagcag caagtaatcc tacaactaag
     8221 aatcccattt tgttatataa gaataaaata atggttgtaa aaattgtacc aacgttatat
     8281 cctaaaggac ctaaaatagt tagagtaatt ggaacagtaa ttgccaaaga catcatagga
     8341 acaaagaaag ttcgaattgg ttttggtgaa tacttgttaa aaattttctc cattgttgcc
     8401 aagaaagaca cacataagat agccgggaaa acctgtgcat tataggcaat gtttggaatt
     8461 gtaaatccaa atagttcaac accttcactt aaagcagttg ttgttgctgg aagcaataag
     8521 tatcctacag cggcaatagc tactaaacga ttacatttca atacattggc agtagttaca
     8581 gctaccataa gaggtaggaa atagaatgtt gcatctgaaa tagcaactaa taatgtatat
     8641 atagtagaat ctgattttaa aatattaaac attgctaata acatcaacag tgattttaaa
     8701 ataccagctc ctgcaatagc aaaaatcagt ggttgaaaaa tattaataat aaattctata
     8761 aatcttgttc ctaagtttat tttttcttca gtttttacag tactatcatt tgtatctaaa
     8821 gggcaaactt ttaaaatttc atcatacaca tcaacaacac tatttccaat gacaacttga
     8881 aattgtgccc cattaactat actcataaca ccttgtactt tttttaaagc atcttgattg
     8941 actttacttc tatcaatcaa cttaaagcgt agtctagttg aacagtgttc taaagctaaa
     9001 atattttttg aaccaccaac ttgttcaata atgtcaatgg cagttttttt ataattcatc
     9061 ttgtaatctc cttctccata attttttatg aatggccatt caagatgcaa aaaaggcaga
     9121 acctatataa acaccttgaa aaatcaaagc aatatattcg atcctgcctg cattaccagt
     9181 aacatgtctt ctatttatac tattttgtat tattatatta acatttgcct aaaagaattg
     9241 tcaatacttt tttaaatttt ctgatactaa ctaatagcac ctttataatt cattttttat
     9301 gtcaattaaa gtaacagtag ccacccacca attagtatca aaattgaacc tggtattttt
     9361 tcttttatat tttctttgaa tataaaatga gcaagtatta aagttattat catagatact
     9421 tgagaaaatg gctctgctat actaagttct agattattta aagcaattaa taatgctaaa
     9481 taagaatatc cattaatagc accacttaaa attgaaagcc cctttttatt agtaaaaatc
     9541 tcacaaatta ttttttgttt ctttttaaat gctaaaaaga taaataaatt taaacctacg
     9601 aaaaaataca gtatagttga atataatata ggagatactt gtacaaaaaa agccttatcc
     9661 aatactcttc ctactgactg caaaaatatg tataaaaaca ttaatctaca tggtaaattg
     9721 ttaaaaatta cagagacatt ctttaaaaaa gaacttactt tttttaatat aaacactcca
     9781 ccaatcataa ttatgattcc tgcaactttt gctaaactta aaccttcacc taaaaatata
     9841 actgataaaa gcagtaaaaa caatgaattt aaactattta ttggagtaac aagtgaaact
     9901 tccccagttg ctaaagacgt tacatacgct actgaaccaa ttgaatagac taaactactt
     9961 atataacatg gtattaaaaa cgaccaatca ctaactctat ccattatgct aaatggaagt
    10021 aaacatactc cccctataaa gaaaaataga aatgatgaag ctatattttt ataaatatct
    10081 ccttcttcat cacctaataa tcttacaact actctttcaa aagctaatag cactactcta
    10141 attaataatg caaaataaga taatatcatt tttgacctcg cttaaaaatt aatccgactt
    10201 attgttatat taactgtata agactaccat ataggtaagt cttttggatt ttttaatatt
    10261 ttatgtttaa atacaggtac attaagtttt tcacctagtt tacggcattg ctctttatag
    10321 gtacctttta atttatcatg aaaaactata caatcaattc ctccttcaat gctatttata
    10381 cacttttcta gtttctcaaa tccagattca tcatcataca ttccacttac aatccattga
    10441 tttggcaata catgtccaat tgatatgtta tattctttat tttttataat atctgagaaa
    10501 gatttctcaa aatgtacccc tccaacacat aacaaagtct tcaaaggttc atcacaatca
    10561 aatactctaa ttaatgattt tgccaatacc tgtattgcta tactattatt aaaactatca
    10621 ctactacttc ctatctcaat atccaacata ggtacagaat attctgtaat taatttagat
    10681 tcttcaccat atgttgttcc agaccagtga gttgcttctg ttaatgtagt aaattcatca
    10741 agtagatttt cattttttat atcctcaata gcattgatta gatttttaaa gtatcttgga
    10801 tttgaatttc caaattctcc tgttggaaca tctccaattg tatgagctgt taggatatgt
    10861 tctggagcat tttctccctc atgccaattt acaactcctg caaaatcata atctctaaaa
    10921 tgtctattta aaattggaag ataatattta taattatgac ttattacatc ttttgttttt
    10981 acaaaatgaa ataagttatt gtttttatcc ttaaactcta atacaaaatt atcatcaata
    11041 acaatatcag tttgatttaa atccattatt tcttcacaac attttatcac attactggct
    11101 actgggtctt tctttaaatc actgcaaaag aaaaaaactg cttttttatt attcatccat
    11161 aaatcacact cctataactt aaatataata tatcttcata cctaaaatca tctaaatatc
    11221 actctaattc cattaaatta tttatatata atatactacc atactcattt tagctatacc
    11281 tttgcctgag tttctatata tatgtgatac atcagcttta aatcttatag aatttccact
    11341 agatacaatg taatcttctc catttatttt aatggttact tcgccactaa aaacagttat
    11401 aaactcttgt gttcctggaa gttcagcatt tgaatctaaa tttccacctt cttctatttc
    11461 aatcataaat atctcaaatc ttcttttgga gtcaaatgga aatattggat aacttatata
    11521 ctttccatta tcttcaataa aatggtctat atcacttttt tgaatcaatt ctatgtcact
    11581 ttcctgcctc tccatcaatg atgtaaatga aatttttagc ccattagtta ttttcttcaa
    11641 agtagatata gtaggattta cctcacactt ttcaatttga gatagcatac tcttacttac
    11701 accactaagt tgtgcaatct tgtctaaact aagatgtttt tctttcctta tcttttttaa
    11761 attttcagca attatttcat ttaaactttg cataaatacc ccctaataca tactaatata
    11821 agataaatta ttttgattat taaataaact tagtaattta atagaaactt tttatccatt
    11881 ataatgtaca ttatgtttaa tatacattac tttttttaga atgtaaatag ttttctgaaa
    11941 ttttgtgaaa atgaataaat aaaaaactag taacctatat ttttacaagg ttactagctt
    12001 ttattataat aatatttcat tttaactata aaacttagat gcacctataa gctgtctctt
    12061 agaaacaatc ttagctaggt ttgctaatga agaaattttt atatctttaa aagaaactaa
    12121 aaatacaaac ttaaaatctg taaattcttg atttatattg tatatatcaa aataacatct
    12181 caaattatca aataagtaat tatcctgtaa gtctgcttct atctcatcag tagcatcttt
    12241 taaatacgct cttacatctt caaataaaac actgaaataa gactcatcta tttcttcacc
    12301 taactcttcc aatatttctt ctatatctaa atcatccata tcaaaagaat ttcctgtcaa
    12361 ctggcaatca aataccaata tattttcttg atttatagta tttgatatat cttgtacata
    12421 ttcgaagtct ggacgtaatt ctaaaatgtc ttttatttca ctgctcttta catggatttt
    12481 tccttttcta ttttcaattt ttaaatctat catatgctcc ccctttcaat ctacaaatct
    12541 ttaaatacct tgttttataa tcttttcaat atctatccaa ttactaactc taactatatt
    12601 ttgatgttta acatctttat tatagttagt atccagaagt attactttaa caccatcatc
    12661 agctagttgc actgagttta aaggattatc ttctataaat atgtcacaac tcagttctct
    12721 tgccttttca actttataat cacttcctaa taagtaaaca tctatatctg aaaatccttg
    12781 tctcgatagc caatttttag ttacatcagt taaacactca tgtctagccg ttacaaaaga
    12841 taaattatgt cctttagata atgtgctaat tacctctgta gcaccctcta caatctttgc
    12901 ttcatcatat gagtgtatat acttattatt aaactccgca tacacatctc ttattcccga
    12961 accatacaat tcttcccaat tatgtgttat acactctgct tcagttatat ttttattaaa
    13021 gatatcattt aggtatggaa gaaaatgata agggcttgtt attgttccat ctatatcaat
    13081 acatatattc aatttgttca tttttacctc catgtaacat ttattgattt gattaatcaa
    13141 atattatagc acagctttaa ttataaatca aaactctgga tatgactata cctatataat
    13201 accaaaaagc ttgcatattt aagcaagctt ttcgtaaatt ttacattttt aactaatata
    13261 taagtttttc taaggttggt tccactccaa attgtgattt cagttcttta tattctttta
    13321 atgctttgtc attattcgtt ttagttttta tcgctcttat cattaaattt tttggtgtat
    13381 caagtggaga tatgtattct actacagaag tatcatatcc attgccctcc aataacaatg
    13441 ttcttaaacc atcagttatt aaatctgcaa atctagcttt aaatacacca tgttttaata
    13501 ttggttccat agcttcatat ctatattgcc ctaataactc cttatgacaa catggtacta
    13561 ctactatagc ttcagatttt gctctaattc ctagacctat tgccatatct gtagctgtat
    13621 cacaagcatg tagacttatt acaaggtcaa catccctatt aggtgtataa ttagtcaaat
    13681 cctcttttat aaatgacata tttttataac ccaagttttt agccatattt tttgatgctt
    13741 ctataactac atctgaataa tctattccta taaaataaca gtttttcttc aatacttctt
    13801 ttatataaaa atttaagaca aagcttaagt aagattttcc acaagcacaa tccaagatag
    13861 ttatacagtc tttatccttt atttctttta aaatactatc tatcaattca acaaaatggt
    13921 ctatctgatt gtattttctt attttatcat tttttatctt accatctttt gtaagtactc
    13981 caatttcttt taaaagtgca tttgcttgtc ctacttttac ataataatct ctgtttttta
    14041 tttgagatgc cgtaaattca tctattttag gtacttcttc cttattatct aaatatttta
    14101 catttacctt cttattgtct ccttcaatta caacaatagt tcctcgctca acatatttaa
    14161 atactaatcc atcataattt tctgcctctt tagaaatatt cttaagcaac tcattaaacg
    14221 tatataaatc tgattttcct ttaaaattaa atttatattt attatcttcc tctattgcaa
    14281 ttcctttaaa ctcttttctt ccagatttaa aaatcacctc taactcaata aaaaaatccc
    14341 tattatcaag gtttctttga gtaatcccca taaaaaacat atttagtttt cctatactct
    14401 gtttattcat cataatctcc cttttaactt atatctttaa atcttattaa ttaatattat
    14461 ttgtttctga attatttatt tcttctttta aatcgttcaa tctattttta aaactttcta
    14521 tctgcttatc tgataaatca agatattctg ttatcttccc tatatattta gggcttttag
    14581 tatttattaa tttcaaagca ctttccatag atttttctct aagctcatat gctttcttta
    14641 cgtcatctct agcattactc aatgtttctg tatacttctc cttagaaagt tctggaatct
    14701 gcatattttc atattctttt acaacttctt tacacttgtt tctggcattt gtaacatctt
    14761 tttttatact agataattta gttatatttt ttgctatatc cttagtgtaa ggtatacatt
    14821 ctctcattgg aactaaagct gctctttcta tattataaat ttctttctta aaaattccta
    14881 tactttccat atctattttt tttatatctt cactattttt ttctttactt gtatatttat
    14941 tattttcttt tgtattatct tcttgttttt ctgatttatt attcactgaa acattactat
    15001 cagaattatt cttttctttt atatcattac ttccttcttc attactttct ttattttgta
    15061 tatttctatc ttgtttgttg tttaaatttt tattatttga tatttcttca ttctttttat
    15121 tatcctgttt taaatcagta ctttcttttt ccttaaaatt tgatacatac ttttcatcat
    15181 atgtctcatc agaaatataa ttgtgataat acatagactc ttttatttta ttaagtccac
    15241 tcatataaat attacttatc attattgttg tagctaatac cacataaata ctactgtaga
    15301 tacaagacct cttaaaattc ttgtaatttc tgaatatcac tactaaagga ataagatata
    15361 aaaatataaa aactaatatc cacctcataa gtcgtgtctc ctttctcaaa accgaaatcc
    15421 ctcaaatatt tatttttaat catgtatctt atagtattta ccaatttatg ttttttaatc
    15481 gtgaactttt attaaattaa attctgtata gtcacaagat gctaatgtat attctactcc
    15541 atttcgaatc cccttcaatc ccattttaaa agattcttta ccatgcaagt gaccataaat
    15601 caccttttca acattatatt cttcaaataa ctttgtaaat aaagattctt ccaatttatc
    15661 attcgtagga ggataatgag taatcacaat tatttttgag tgaccacttt tacgagcaga
    15721 ttctaatgat agtctaagtc tatgttcttc tcttttataa actttttcat cagtctcatc
    15781 aaatttaaca tcattaggac atatccagcc cctacctcca caaattgcat aatctttata
    15841 ttcataaaaa tttgtctgaa taaaacgcat gtcttcatat agcttattta aacttgtaac
    15901 ggttgtccac cagtaatcat gatttccctt tataagtatt ttctgacctg gaagtttgct
    15961 tattatatct aaatcttttt tagcttcgga taaattaatt ccccaagatg tatctcctag
    16021 tactaagacc atatcttctt ccttaacaac ttctttccaa ttatctataa ttttcttttc
    16081 atgaccatcc caattacttc caaagatatt cattggctta ttgactgatg ttgaaaaatg
    16141 taaatcacct atagcataca aactcataat atcattcctt tcttaattac actaaatagg
    16201 ctgtctcttt ttagaaatta ttttccatta agaggcagcc ccattaatat ttagcaaatt
    16261 ttctacaagt agtttttact gaactacttt atcatctacc ttagtatcct tatattgctc
    16321 taataatata tctatttctt gttttaattc caaaatttca gttatatttt tatctgtttc
    16381 agttctatca agttttcttt ggtactgcat agctttatgt gtatcaccca agtcatataa
    16441 ctcttgtatt ctatttaaag tatctctaat agctatagac ttcatttggt acatagcctg
    16501 agctttcatt atgtcctctc ttatttcact catttcttgg tctaaaagaa aggcagcatc
    16561 agatgctctt ctaagccttt cagcaagagt ctcaggtatg taatggtcat atttatattt
    16621 taatgaatgt tctattgtag cccagaaatt cattgcaaga gttcttattt gtatttcaca
    16681 tataatttcc tttgaacccg caatggagtt tatagggtat tttattataa catgatagct
    16741 tctataacca ctatctttgt aatttgtaat ataatctttt tctcctatta tagtcatatc
    16801 atttcttact tttatcaaat caactatagc atatatatcc tcaacaaact ggcacattat
    16861 tcttatacca gcaatatcat caatttcagt ttctatatct tttgcattta tcctttttaa
    16921 cttagatatt atggaagata ttttttttgt tctacctgtg acaaactcta tgggagaata
    16981 ttcacctttt gctaaatatt cttttcttat atttttaaat tttattttca attcctctac
    17041 tgcatgttca taaggtgcta gtatctcatt ccatttttcg tattccatat tgctcaccct
    17101 ttatttgttt tttatgacct tctttatttt atcataattt attttgtttt tgtatagtaa
    17161 agcaattctc cattttccaa tatataccct atttcttcta aatcaactaa gtaacttatg
    17221 acagatacta acgaagattt aaaaaaatga tactctttat aattattgct taaattattg
    17281 ttatttataa tgttttttaa taaaatttct aaagatttcg gttctacaag caactctctt
    17341 atttgatata aatatttttt taaagcattc aaatgttttt ctattaattt ataagaatct
    17401 tctttcgata taacttgctt gccatgccct aaaactaagt actcaaaatc tatatttttt
    17461 aatttttcta atgaatttat ttgttcttgt atatcatata gaaacaagaa atcgtatttt
    17521 gatagaattt catctcctat caataaatcg ccaacaaaaa tcacattatc cttcgtcaaa
    17581 acagctatac taccaggtgt atgaccctga aattcaatta tatcaaacat ttcattgttt
    17641 aattcaattt ttccagcttc tatagattta tctattttta tatcatccaa cattttattt
    17701 tttaagatat tatccataaa tacattgctc tttcccccaa caatatactt tgaaaacaat
    17761 tctggatttt ctatatataa tttatcgtat tcagatgaca ttatacacac atctttataa
    17821 tattctttaa attgattaca agctccatag tggtcatcat gaccatgagt atttataata
    17881 tatctaacct gtatattatt ttcatctaat actttattta ctctcctagg tcttgaacca
    17941 cataaccctg ggtctatcat aagtgctgtg ttatcttcaa atacatatat tcctgtattc
    18001 gtgccacctt ttataaaaaa ggtatttcct tttacttttg taagattcac cttcttcacc
    18061 tcattttgac aatatttatg atacaattta ttacttacta atataaaact atacaagaac
    18121 aatccttttt attctatcta tagttctttt tcttattttg tattctttag atatccttat
    18181 tttcacatta tatgctaaat ttctataaag taatgactct ataaaatatt caactatacc
    18241 tctatcccta aagtctatat ctatatttcc acttgaataa atatttgagt ctaatatcat
    18301 aagctcatca aaaaagctta atctcttaga tttacttaaa taatctttat ttggaataaa
    18361 tctatcacaa taatctatcc tgtaatttac tacttcatca tttatttcaa atccatattg
    18421 ctttttcatt aactctctat agttattatt tttctcaata acattatcta aatttttatt
    18481 ataatcctct tttttccaat gtccaaacat cctacaattt aatggtctaa cttcatatat
    18541 taaacatcta ttattagtat ctttaaatgg acatgggttc tttcttgtat actcctcaaa
    18601 ataataatct aatattttac ttatacagct tttttttaaa tcaactctat cttcaagata
    18661 acaaaatata ttcaagaact caattaaatt tatacctact gactccatac aacaatttcc
    18721 acatccagaa caatttccta ctggcaagga cttatataca tcatttagtt tgtcaaagag
    18781 cttattttta atggaataat ctatacaatt taaaatatct ttatttttta tactagtcat
    18841 tattattact ccttgttata attattaagt taatacaaac aattattata acatattttt
    18901 taaatttaat cgcatatgga ggtattttat gaatagatat acaaaaataa ttaatatgat
    18961 gggaagttat tacacaaaag actttgaaaa agaaaaaaag aatgtaataa aagttagaga
    19021 agttaaagaa gatacagtta gaaaattttt cttacaaggt gactgtgaag ttcttgtagt
    19081 atttgaagac actgggaaag aaatacttat tgatgacttt tctcctgaag aagatatcaa
    19141 aaaatat
//
LOCUS       DAGSRM010000076        18824 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.076, whole genome shotgun sequence.
ACCESSION   DAGSRM010000076 DAGSRM010000000
VERSION     DAGSRM010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18824
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.076"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            424..1584
                     /locus_tag="MJE33_002728"
     CDS             424..1584
                     /locus_tag="MJE33_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBY9648656.1"
                     /translation="MIETGFVYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGTYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGADSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPKFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISKLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALMVTQILHMLA"
     gene            1926..2087
                     /locus_tag="MJE33_002729"
     CDS             1926..2087
                     /locus_tag="MJE33_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648657.1"
                     /translation="MIIPSRRIQDITLTNLKNGEVTLIELDEIYKKMGFLFVVSEGKL
                     KKIKKENHH"
     gene            2280..2909
                     /locus_tag="MJE33_002730"
     CDS             2280..2909
                     /locus_tag="MJE33_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBY9648658.1"
                     /translation="MKIVTYNIHKGMDSNNRLTLTKMGLYLKQLDCDVICLQEVLYPQ
                     FLALKAVLNMDGVFATNVKKVNMIYGICTFTTSKMLNSNHFFLTSKKEQRGALCITID
                     AYGRIINVINTHLGLDRQERAKQLDEIIDYRNRLVGIVILCGDFNEKNVFLSMFNDMA
                     ISLNKSYLPTFEKSNSRIDYIFVNKNTELKGYTVEKIYLSDHYPVIGYI"
     gene            3148..4407
                     /locus_tag="MJE33_002731"
     CDS             3148..4407
                     /locus_tag="MJE33_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBY9648659.1"
                     /translation="MGRRTTRVSRLGKEKKRSVLIVLAALVLIMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKAEALKAITDKYTPEDLNLAYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLSYTDSVVGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTVYNAALLSGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVKQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
     gene            4666..5661
                     /locus_tag="MJE33_002732"
     CDS             4666..5661
                     /locus_tag="MJE33_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HBY9648660.1"
                     /translation="MYEEVTWLNFIGNRYEVVNSDDVLEINKIYKARDVFYRKNVLIK
                     VIKHNNYICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGI
                     GLDELILGNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDF
                     GITKANNGVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNS
                     EEKMLQSIDKGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKA
                     YIEVEDEKEEEQKQDKVIQVKKEYKSKMRKKLHKKLVVAGLAIVMISMIIISSII"
     gene            5907..6974
                     /locus_tag="MJE33_002733"
     CDS             5907..6974
                     /locus_tag="MJE33_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBY9648661.1"
                     /translation="MSNIKIIASNKCWIEDIARKQLEDISKLNGILRVVGLPDLHAGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGVLKKKFKKDKWIKSLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFVEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYDKDGLEVESEKAIDYIKKHDNALMWAERNREAISKKIMQSIGTSGDVET
                     IFSINHNFVEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTEISLYSLSH
                     GAGRKWSRSVCKSRLKSKYSKDTIKQTKFKSQIICNDVNLLFQEAPEAYKNIEQIIES
                     LIEYELIQVVATMKPLITYKG"
     gene            7508..9418
                     /locus_tag="MJE33_002734"
     CDS             7508..9418
                     /locus_tag="MJE33_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBY9648662.1"
                     /translation="MNSNTFERLQLNEVKELIKIHCVSSLGKNLIDKLTPSGNIGVVR
                     RKLAENKEARKIIENSNHIPLEGLFNAGSTIDKIEKGMIIEPVELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKSIEDEINYCIKSNKVDSNASKELKKIRRNIEIT
                     EGKIKDRLNKFITSTVNKKYIQEFIISKRNDRYVIPIKSSYKNEVNGTILDTSSKGNT
                     VFIEPISVSNLSTELTMLKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMLF
                     AKAKYSQKIKGITPKINNNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAG
                     GKTVTLKTVGLLTLMVQCGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIK
                     NIAEIMSLSNNSTLVLFDEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKK
                     FATLHPEFENAGMMFDKETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVI
                     DRNYNLDLVKRSKLQSNIEDKNSSEVILNYTDYNVGDKVKLLDEDDFGIVYKPMDKFN
                     NVEVLFNDNFVSVNIKRLQLSIKASELYPEGYDLDVLFTSFKERKLEKDIQRGSKKAL
                     KKIQKEIRENRK"
     gene            complement(9481..10104)
                     /gene="ytaF"
                     /locus_tag="MJE33_002735"
     CDS             complement(9481..10104)
                     /gene="ytaF"
                     /locus_tag="MJE33_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HBY9648663.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFLLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLKLNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLLGCLLGNMFVKNVNLNLSWLSGLILLVIGFMKIC"
     gene            10292..10936
                     /locus_tag="MJE33_002736"
     CDS             10292..10936
                     /locus_tag="MJE33_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY9648664.1"
                     /translation="MEQNNKVGNFGDLLKILLDEKELSMGELSKKSGIDKSTISRIAN
                     NKQKPNINHLEKMAIHLNINLEELLKASGYEFRNSNNQIFNADSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLRQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLKNDK"
     gene            11150..12208
                     /locus_tag="MJE33_002737"
     CDS             11150..12208
                     /locus_tag="MJE33_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HBY9648665.1"
                     /translation="MNCKWISKIDERKKCHREADSSGYCIFHKENKSDEEIQLMMDTL
                     HKEEISEFNGFVFENEFNAEEILTYNYKILDFSESIFKQKANFKKYIFKKNIIFNYTE
                     FRDKVLFNGCVFLENCDFNRTIFSKHYINDRIFEKVKFKGPDLVVNKVENFPRMDGII
                     FSMCTKFVLKNVEYGKSEYEHGKINYRIARNQATKIGEYEMIGFYYYKERIYSSKIMK
                     RSNYPTFSDYLVEKFFDQIARYTTGYGEKPWNILLVIIAIISVFALLYLFVGIESSNS
                     TLVALDINNIGDYSLSEIFKMYMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEVFF
                     GVTIGAIWASVIIKRMIR"
     gene            12312..13496
                     /locus_tag="MJE33_002738"
     CDS             12312..13496
                     /locus_tag="MJE33_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBY9648666.1"
                     /translation="MKNALKKIISLAMILVFIIPNYSYADDKSVDKYSKTSILIDQET
                     GRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSSYKL
                     KDGEEVPLIELMRGLMIVSGNDAAIAIAKHVCKTKEAFVDRMNKKAKEIGMSNTHFLN
                     PNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDHYEKQVTAITDMETYTNSDRSFE
                     KSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMVVNKNDKNEKKRRVIGVVLGTNHKN
                     KRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGTKELEVTLKAKDELYTILKDDE
                     SIKSEVKIKNIEYPVKKGDTMGIIKYYTNSGDLLGSVDIVSNNSIDNVSLKTKLKMKF
                     AN"
     gene            13703..14416
                     /gene="rpsB"
                     /locus_tag="MJE33_002739"
     CDS             13703..14416
                     /gene="rpsB"
                     /locus_tag="MJE33_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBY9648667.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            14495..15406
                     /locus_tag="MJE33_002740"
     CDS             14495..15406
                     /locus_tag="MJE33_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBY9648668.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            15491..16195
                     /locus_tag="MJE33_002741"
     CDS             15491..16195
                     /locus_tag="MJE33_002741"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBY9648669.1"
                     /translation="MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            16221..16778
                     /gene="frr"
                     /locus_tag="MJE33_002742"
     CDS             16221..16778
                     /gene="frr"
                     /locus_tag="MJE33_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBY9648670.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEEFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDTLLSKKEKDIMEV"
     gene            16781..16984
                     /locus_tag="MJE33_002743"
     CDS             16781..16984
                     /locus_tag="MJE33_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648671.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDEQNINYTVKSIEGKKDKEKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            17013..17747
                     /locus_tag="MJE33_002744"
     CDS             17013..17747
                     /locus_tag="MJE33_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBY9648672.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            17760..18539
                     /locus_tag="MJE33_002745"
     CDS             17760..18539
                     /locus_tag="MJE33_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBY9648673.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGIPLYIAETAIVYIALHEFYKAF
                     KIKDVHPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINGFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
BASE COUNT         7762 a         1866 c         3244 g         5952 t
ORIGIN      
        1 tacaaatgtt ctgaaaacta aaattaaaag aatagaatct aaacatattt aaaaaaaaag
       61 tatataaaat aaatttgatt tatattatat agatttaaag taaaaatagt tttatttaat
      121 aattaataaa aataaataca aaacaaaata aataaataat aaaatattta gtttaattat
      181 ctttacaaat aaaaatttta gtaataaaat aattgttgaa aaagatttta aattcagatt
      241 attcatttct aaagtaatta atgtgcaaat ctttattaat atatgtcaat agttaggtat
      301 atatgtattt aattagtatg atatagataa aagtatttat tttaattgtc tattatctga
      361 aaaagtcttt acttagaaat cataaaaatc aaattaatta ggaaaagaaa gggtgtgtat
      421 aatgtgattg agactggatt tgtatattgt agtttcttgg ttgtattcgt aggtgtaatt
      481 ttttttatgg ttgataaatt gaaatggaag gtatttgatg ttattaatcc aatgctttta
      541 atatacttag gttcagctat acttggatca tttggtttat ttgcacagaa tgaagatgtt
      601 ggaacgtatc aaaacataat aacagtgaac ttccttccat taatgttagt gtttttgtta
      661 attcaatgtg atttaagaaa aatattaaag atggggccta aaatgttatt atcatttttc
      721 tgtgctacat ttacatttat gcttggattt gttatagcat tcataatttt taaaaattca
      781 atgggtgcag attcatggaa aattcttggg gcagcatctg gttcatggat aggtggctca
      841 tcaaatatga ttgcagcagc taatgctcta ggagttgcag aagaaggact tagttatgca
      901 atacttatgg gttctatagg atatacagta tggatgtctg tttgtctaat gtctgtgaaa
      961 tttggaccta aatttaacaa atggacaaag tgcgatacaa gttttataga tgaggttaca
     1021 tcaaaacttg aaaaagaaaa taaagataat acggcaccta cgtttgtaga acttatgatt
     1081 ttactttctg ttgggtttgg agttgcgtct tttgcacaaa ttgtatccaa gatacttcca
     1141 acttcaggta tacttaatga aactatgtgg gtagtacttg ttgcatctac agtatcaata
     1201 ttattgggta tgacaaaaat atcaaagctt aaagggagta cactggtagg aaatgtgttt
     1261 atgtatttac tccttgcagc tacaggtagt aaagcaaatt tctttggatt gtcagaagca
     1321 cctgtttaca taatgtttgg attggtagta gtccttgtac atgctatttc atttataggg
     1381 cttgctaaac tatttaaact tgatttattt acttgtgaag taggaagtat agctaatata
     1441 ggaggagtaa ctacagctcc aatgattgct ggggttcaca atcctgtatt aataccagta
     1501 ggtgtactta tggggcttct aggaaatatt atagggactt attgtgctct tatggtaact
     1561 caaatattac atatgttagc ttaataaaaa tatggtataa aattaataat ttaaataaga
     1621 aaagtcattt caatttaagc agaagctttt actgaaatgg cttttttgtt tgacaagata
     1681 atctatttca agaagatgtt ttgacatatt tagtgtataa tattgtaaaa tttactaaaa
     1741 acgagatata tttaattttt atattgaatc gaacgtctgt atgatgtata ataaatatac
     1801 agaacgaatg tttgtagaac gggtgttttt tgaatttatt ttaataattt atgaatatat
     1861 atcagaatgc ataaatatat ttaaatataa agattaaaaa gaatttaaag gagtgttgat
     1921 aacttatgat aattccatca agaagaatac aagatataac attaacaaat ttaaaaaatg
     1981 gtgaagtaac tttaattgaa ttggatgaaa tatacaagaa gatgggattt ttatttgttg
     2041 ttagtgaagg caaattgaaa aagataaaaa aggagaatca tcattagaaa tgaatgaaat
     2101 aataaatatt ataatagttt ataaaaccta acttatgtag aatataaata cttaagttag
     2161 gtttttttgt gataaagttt ataagtactc aattttataa gataaataat ggtcttaagt
     2221 gaaaagtaaa taagtttaaa ttttagtaag tattatattg cataagaggt gatattatta
     2281 tgaaaatagt aacctacaat attcataaag gtatggattc aaataataga ttaactctaa
     2341 ctaaaatggg gttatattta aaacaattgg attgtgatgt aatttgtttg caagaagtat
     2401 tatatcctca atttttagct ttaaaagctg ttttaaatat ggatggtgtc tttgcgacaa
     2461 atgtaaaaaa agtaaatatg atatatggta tttgtacatt tacaacatct aaaatgttaa
     2521 acagtaatca ttttttctta actagcaaaa aagaacaacg gggagctctt tgtataacta
     2581 tagatgcata tggaagaatt ataaatgtaa taaatactca cttgggactt gataggcagg
     2641 agagagcaaa acaattagac gaaataatag actataggaa cagattagtg gggatagtta
     2701 ttttatgtgg tgattttaat gagaaaaatg tctttttaag tatgtttaat gatatggcta
     2761 tttcactaaa caagtcttac ttaccaacat ttgaaaaatc aaactctaga atagattaca
     2821 tttttgttaa taaaaatact gaactaaaag gatacacagt agaaaaaatt tacctgtcag
     2881 accattatcc tgtaataggt tacatttaaa tttcaatata aatgaaatta gttgttataa
     2941 aataaacaat tttttgttaa gaaatagtaa aagaaatata ataaaaaata tcaaaaataa
     3001 aactaattat aagacataaa tttacaataa agttacattt gattaaattc cttgtttaac
     3061 aaaagctatt caaatataat ctaaggtagt tttgaaatgg gaactctatt atgcaaattt
     3121 aagctagtgt taaaatgagg tgaagttatg ggcagaagaa caacaagagt ttcaagatta
     3181 ggtaaagaaa aaaaacgaag tgttttaatt gtattggcag ctttagttct aataatgggt
     3241 ggatttactt atttttttaa cagtaaattt ctgtacaatg ggaaaatcgc taaaaatgta
     3301 tatatagaag gagtaaatgt atctgatatg actaaagcag aggcattaaa agctataaca
     3361 gataagtata ctccagaaga tttaaatctt gcttatgatg ggaaaaaata tacaatatca
     3421 ccaaaagata ttgatttaaa atatgataca gaagatgttg taaaagatgc ttatgaaagt
     3481 acaaaaaaag ggtcctattt tcaaaactta aaaaaatata ttgatataag agtgaataag
     3541 gcaaatatga agataaaagc tgaatatgat gaggcaaaac ttagttccaa agtgtcttct
     3601 atagcagata gtataaatgt taagatgaaa aatgcttcta ttagtgttgg aagtggtgga
     3661 ctaagttata cagactctgt tgtaggtaga gaatttgatt tggcagcaaa taaagagtct
     3721 atatataata tgataaaaaa taaagagcat aaaactttag aacttaaagt aaatcttcaa
     3781 aaaccagata taacaactga acaagtaaaa actgtaaatt ctgttatagg tcaatattca
     3841 actacttatt cacaagctgt agagggtagg tcatataatg taggattatc agcaagaaaa
     3901 actagtgatg tattattaat gccaggtgaa gaattttcat acaataaact tacaggacct
     3961 agtaataaag caaatggtta caaagatgca cctgttatag tttatggtaa gctagaacaa
     4021 tcagcaggag gaggtgtttg tcaaacatct tcaacagttt ataatgcggc tcttctttcg
     4081 ggtatggaga taactcaagt tacaaatcat tcatctgcat ctacttatgt gcctaaaggg
     4141 agagatgcta ctgtgagcga tggaggatta aacttaaagt ttaaaaatcc atataagcat
     4201 cctgtatata ttaaaaatta tgcaggtgga ggaagtgtat catcagtaat ctatggtaat
     4261 tctggtgaca aaccaaatat atctatagag gtaaaacaaa ctggtcagaa taaatattct
     4321 acatatcgaa tatttaaaga ttcaagtgga aaggtcataa aaaaagaaca tatatcaaat
     4381 agttcttata aagaattaaa aaaatagata attttttaga gaaatatttg taatataaaa
     4441 gggagtgtct caaaatgaac tttttagttt atgaggcact ctttttctgt gaaatcaaat
     4501 atattttcat catttcaata atacaaaaga agcgtatctc tattttgaga cagccttttt
     4561 tgtaaaaatt aacactatta gttcttgttt taatattaca atcattaata tatagaaaat
     4621 attcctatat attggtataa ttgtaaatag atttagttat ataaaatgta tgaggaggtg
     4681 acatggttga attttatagg caatagatat gaggtagtaa attctgacga cgttttagaa
     4741 attaacaaaa tctataaagc tagagatgtt ttttacagga aaaatgtgtt aataaaagtt
     4801 ataaagcaca ataattatat ctgtgaagat tttgtttcaa atttaattga tgaaagtact
     4861 gctttagatg agataaactc tccttatata ctaaaaataa tagatgttgg gattcactgt
     4921 acagaagaaa caacattata ttatgttgta agtgaagact ttaatggtat cggactagat
     4981 gaattaattc taggtaatta tcttcattta gaagcaattg taaacataat gatacaagtt
     5041 ttaaaagctt tagaaatgat acataggaat cattcatatc atggtagttt aaaatcgagt
     5101 agtataatag tagatacaga atacaatata aaaatttgtg attttggaat tacaaaagca
     5161 aataatgggg ttaatacaag aagctatgga aacaggagat atttatgccc acatcaacta
     5221 tgtataaatt atactgataa agaaagtgat ttttttgcaa caggtcttat tctatttgaa
     5281 tctatattta aaaaattgcc ttttggagaa tcaaattcag aagaaaaaat gctacaatct
     5341 attgataagg gattagattg gaatagtata aaagcaatta atggaaatac tgaacttata
     5401 aatgttataa aaaggcttct tggaagaaat aataagtata ccagagccaa tgatattatt
     5461 attgatttaa gtaaagtaat gtatgaaaaa gcatatatag aagttgaaga tgagaaagaa
     5521 gaagagcaga aacaagacaa agttatacaa gttaaaaaag aatataaaag taaaatgaga
     5581 aaaaaattac ataaaaaact tgttgttgcc ggtctagcta tagtcatgat ttcaatgatt
     5641 attattagtt ccataatata aaaattgtat attaagtatt ttaaaatcaa tgtctataaa
     5701 ggatatacat agaaatttta gtgtagtctt aaattatgaa aggaagtggt tattatgtta
     5761 acaaaaaata atgaggtatt aaacgtctag gagtatttta acaaatagcc acctgctttt
     5821 acagtttaga ataaaaaagc tgtggctaaa ggtcacagct ttttgctact ttttaaaggc
     5881 aggagaatat taaaaaggag caaattatga gcaatataaa aataatagca agtaataaat
     5941 gttggattga agatatagca aggaaacaac ttgaagatat ttcaaagtta aatggaattt
     6001 taagagtagt aggattacct gacttacatg caggtaaaat acctgtaggt ttagcagttg
     6061 agactaaaaa tattatttat ccacatataa ttggaaatga tataggatgc ggaatgactt
     6121 tatttaaaac tggagttcta aaaaagaaat ttaaaaaaga caaatggatt aagtcactta
     6181 gtaagattaa agatttgtct gatatagaaa taaaaaacac atataaagaa gaatgtccaa
     6241 ttttaaattt aggaactata gggagtggaa atcattttgt agaaattcaa tgtataagtg
     6301 aaatttataa taaagaacaa tttgaacaat taaaattttc atctgatgat attatgatgt
     6361 tagttcactg tgggtcaaga aattatggag aagatatttt aaaaaaattt tatgataaag
     6421 atggacttga agttgaaagt gagaaagcta tagattatat taaaaaacat gataatgctt
     6481 taatgtgggc agagcgtaat cgtgaagcta tcagtaaaaa aattatgcag tcaataggta
     6541 catctggaga tgttgaaaca atattctcta taaatcataa ctttgtagag aaaagggaag
     6601 ataaatttat acatagaaaa ggtgctgttt caagtgaaag aggagctgtg attataccag
     6661 gttcaagagg cagtctatca tatatagtta tgcctactga aaatacagag atatctttat
     6721 attctctatc acatggagca ggtaggaaat ggtctcgttc agtttgtaaa tctcgcctta
     6781 aaagtaagta tagtaaagat actataaaac aaactaaatt taaaagtcag attatatgta
     6841 atgatgtaaa tttattattt caagaagctc cagaggcata taagaatata gagcagatta
     6901 ttgaaagttt gatagaatat gaacttattc aagtagtggc aactatgaag ccattgatta
     6961 cttataaagg ataattttta aagttaaaaa agattaaaat tatattataa atagctaatt
     7021 ttatggaaca tttaaaagtg ttttattatt agctattttt ttattttgta gagacgataa
     7081 tttatagagc taaattcttt gatatataat gattacaaag ttagactgta ttggaaactg
     7141 cacatacatg aattgatgac aatataagtg attccaaaat ataaatgaaa gtataagctt
     7201 attttcacta taaaaatttt cactgtacaa aaacattaat gacattattt ttattgacat
     7261 aaatatataa tttatggaat aatattagaa gcactgatat aagtgtttaa ataaggatag
     7321 aaaaggagaa gtgttttatg aaaaaaaata ctaacattaa tgagtccgga gttaaacttc
     7381 ttaataaata aggagtttga tatataataa ctccttattt attaagttgt tcgcaaaata
     7441 actattatca aagtaaatga tagggtgatt taaaagacga taacttataa ataaaggaga
     7501 aaaaataatg aatagtaata catttgaaag attacaacta aatgaagtta aagaattaat
     7561 aaaaatacat tgtgtaagtt cactaggaaa aaatttaata gataaattaa ctcctagtgg
     7621 gaatattgga gtagtaagaa ggaaattagc agaaaataaa gaagctagaa agataattga
     7681 aaatagtaat cacataccac ttgaaggatt gtttaatgca ggttctacaa tagataaaat
     7741 tgaaaaagga atgataatag aacctgttga acttgtaaat attgaagatt ttttaagagg
     7801 ttgtaggaag atgaaagctt ttatgttaga aaaagaattt tattcaccta cattaagttc
     7861 ttatgcctta aatataacag aatgtaaaag tatagaagat gagataaatt actgtataaa
     7921 atctaataaa gttgattcta atgctagtaa agaattaaaa aagataagac gaaatataga
     7981 aattacagaa ggaaagataa aagatagatt aaataaattt ataacatcaa ctgtaaataa
     8041 aaagtatata caagagttta taataagtaa aagaaatgat agatatgtaa taccaattaa
     8101 atcttcttat aaaaatgaag ttaatggtac tatattagat acatcatcaa aaggaaatac
     8161 tgtatttatt gaacctatta gtgtttcaaa tttaagtaca gagttaacta tgctaaaagc
     8221 tgatgaaact attgaagaat acaaaatatt atcttattta acagaactta tatttgagaa
     8281 gataagtcag ataaaattaa atatagagat actttcagaa tacgatatgt tatttgcaaa
     8341 agctaagtat agtcaaaaaa taaaaggaat tactccaaaa ataaataata atggctatat
     8401 aaaaataatt aaaggtaaac atccacttct aacaggagat gcagtacctt tagattttga
     8461 aataggcaaa aattatagaa gtcttataat aacaggtcct aatgcaggag gaaaaactgt
     8521 aacccttaag actgttggtt tactaacttt aatggttcaa tgtggatttg atatttctgc
     8581 aaaagaaggt agtgaattta gtgtatttga aaaagttttt gtagatatag gggataatca
     8641 aagtattgaa aatgccttaa gtactttttc atcacatata aaaaatattg cagagattat
     8701 gagtttatca aataattcta ctcttgttct gtttgatgaa ataggaagtg gaactgaacc
     8761 taatgaagga gctggtttag caatatcatt attagaagag ttttacaaaa tgggatgtat
     8821 aactatagca tcaactcatt atggagagat aaaaaagttt gctacattgc atccagaatt
     8881 tgaaaatgct ggtatgatgt ttgataaaga aacgttagaa ccattgtata agttaactat
     8941 tggaaaatct gaagatagta atgctctttt tatttctaaa aaaatgggaa taaaaaatag
     9001 agttttagat agagctaaag aatatgtaat tgatagaaac tacaatttag atttagttaa
     9061 aagaagtaag ctacaatcta atattgaaga taaaaatagt tctgaagtca ttttaaacta
     9121 tacagattat aatgtagggg ataaggtaaa attactagat gaagatgatt ttggaattgt
     9181 atataagcct atggacaagt ttaataatgt agaagtattg tttaatgata attttgtttc
     9241 agtaaatata aaaagattac aacttagcat aaaagcatca gaattgtatc cagaagggta
     9301 tgatttagat gtattattta ctagttttaa agaaagaaaa ttagaaaaag atattcaaag
     9361 aggttcaaaa aaagctctta aaaagattca gaaagaaatt agagagaata gaaaataaat
     9421 ataaaaagca caaaagaaag caatctaaag agaaaagttt atctttagat tgctttctta
     9481 ttaacatatc ttcataaatc caatgactaa caagattaaa ccacttaacc aagataagtt
     9541 taaatttacg tttttaacaa acatatttcc tagtaaacaa ccaagtaata ctgcaattat
     9601 atttgatatt aaagaaaata gaataacttg tatataattc acacctacaa ggccactacc
     9661 aaaaccgata gctaatccat ctaaacttaa agcaagtgca agtgtaaatg cttcagcaga
     9721 tttaagcact ttagatttac ttgtatctaa gacatcaggt tcgatactta cattaaaatt
     9781 taatttaagg tctaacatat taaaattata attctttgag tgtatagatt ttttcttaaa
     9841 ataagattta aaaaaacttt caattagtct agcacttcct aataaaaata aaataaagaa
     9901 acatattaac attgttatat tttgaggtaa aaaatctcta actattgtac ctgcaaaaag
     9961 cgatattgct aaaatagctg aacaaactac gctgataatt atatttgaat ataagggaac
    10021 tttaacttta tttacaccat atccaaagct tgtcacaaag gcatctatac aaagagaagt
    10081 aactaataaa attgattcaa tcacagtaga ttaacatcct ttctcttaaa ttttgtactt
    10141 atattagtta tagtattgta aaaatggata aattgttaca gataatccaa taaattgttg
    10201 ataaaattcc aacatcatac aacacctaaa taacaaaaga atcatatcat ataataatat
    10261 aaaaccaatc ataataaagc gggtgaaaat aatggaacaa aataataagg ttgggaattt
    10321 cggtgatttg ttgaagattt tattagatga aaaagaatta tcaatgggag aactaagtaa
    10381 aaaaagtgga attgataaat ctactatatc tcgtatagct aataataaac aaaagccaaa
    10441 tataaatcat ttagaaaaga tggcaataca tttaaatatt aacctagaag aactgttgaa
    10501 agcttcagga tatgaattta gaaacagcaa taatcaaata tttaatgcag atagtgattt
    10561 tagtaacttt gatgatattc ttggatttgc aaatttaatc aatgataaga actttaatgg
    10621 aaatattgaa aaagagttaa gtaagtgtaa gttatatgtc caaacagatg aaggtaaaaa
    10681 attacttttt gataatttta ataaaaaaat agattctatt gaaaaacaag gtcaatttac
    10741 agatagatta agacaaatgt atgctgattt ttgtacagat ggacttacaa taaagaaata
    10801 tttattgatt ggaagtatgc tactttactt tgttatatca acagatgtga ttccagactt
    10861 cgtgttccca ataggattta tggatgattt agtagcatta aatatagtta caaaattact
    10921 taaaaatgat aagtaagttt tagaatcata tttaatatat aaataaatca aagtatataa
    10981 aatttattat tttttatatg aaacaaatta aaagcacaat gaagtagatt aaaaatttaa
    11041 aactaagaat ctaatatata ttttgtaact atttaagtat ataaaataaa ataatatcca
    11101 aactaataca aaatagtaat aattgttttg tactggagga tatatattta tgaattgtaa
    11161 atggatatct aaaatagatg agagaaaaaa atgtcataga gaagcagatt cttctggata
    11221 ttgtatcttt cataaagaaa ataagtctga tgaagagata cagttgatga tggatactct
    11281 tcataaagaa gagataagtg aatttaatgg atttgttttt gaaaatgaat ttaatgcgga
    11341 agaaatatta acttacaatt ataaaatact ggatttttct gaatccatat ttaaacaaaa
    11401 agcaaatttt aaaaaatata tttttaaaaa gaatattatt tttaactata cagaatttag
    11461 agataaagtt ttatttaatg gttgtgtatt cttagaaaac tgtgatttta atagaaccat
    11521 atttagtaaa cactatataa atgaccgtat atttgaaaaa gtgaaattta aaggacctga
    11581 tttggtagtt aataaagtag aaaattttcc acgaatggat ggaataattt ttagcatgtg
    11641 tacaaaattt gtgttaaaga atgttgaata tggaaaaagt gagtatgagc atggcaagat
    11701 aaattataga atagctagaa atcaagcaac aaaaattgga gaatatgaga tgataggatt
    11761 ttactattat aaggaaagaa tttatagtag taaaataatg aaacgttcta attatcctac
    11821 cttcagtgat tatttagttg aaaaattttt tgaccaaata gcacgttaca caacaggtta
    11881 tggagaaaag ccatggaata tattattagt tattatagca ataataagtg tttttgcttt
    11941 attatactta tttgtaggca tagaaagttc aaatagcaca cttgtagctt tagatataaa
    12001 taatataggt gattactcac taagtgagat tttcaaaatg tatatggatt tatggtattt
    12061 tagcatggct acatttagta cagtaggata tggagatatg gttgctacaa gtttgattgg
    12121 gaaagcattg gcaggtatag aagtattttt tggagttaca ataggagcaa tttgggcatc
    12181 tgtgattatt aaaagaatga taaggtaata tttgtgtaaa tatggatagg tattaattac
    12241 tagatataaa ttggactaaa tagggaaaat ttaaatataa atatacttaa taatccaaga
    12301 aggtgaaaaa tttgaaaaat gcactaaaaa aaattatatc attagccatg attctagttt
    12361 ttataatacc aaattattca tatgcagatg ataaatctgt agacaagtat tcaaaaacat
    12421 ctatacttat agaccaagaa acaggtagag ttttatatag taaagaacct gatatgaagg
    12481 taccattagc tagtttaagt aagatgatga cttttttact tgcaatagaa gctattgaaa
    12541 ataaagaagt aaaagaaact gataaggtaa aaatagacaa atctattgca tcagtaaaag
    12601 ggtcaagtta taaacttaaa gatggtgaag aagtgccact tatagagctt atgagaggtc
    12661 ttatgattgt atcaggaaat gatgcggcaa ttgctatagc aaaacatgtt tgtaaaacga
    12721 aagaagcatt tgtagataga atgaataaaa aagcaaaaga aataggaatg agtaatacac
    12781 attttctaaa tccaaatggt cttccaatat acgatttaag caaccccaaa aaaccagcaa
    12841 aagaaaatat ctctactgca aaagatatag ctattcttgg aaaatacatg tttgaccatt
    12901 atgaaaaaca agtaacagct ataactgata tggagacata tacaaattca gatagaagtt
    12961 ttgaaaaaag caatacaaat gcattattaa gaattatacc agaagtagat ggtataaaaa
    13021 ctggatatac tggcaatgct ggatattgct tatcattttc tatggttgta aataaaaatg
    13081 ataaaaatga gaaaaaacgt agagtaatag gggttgtatt aggtactaac cataaaaata
    13141 aaagaacctc tgcttcatta gctctactga aatatggaaa agaaaatttc aatatgaaaa
    13201 aagtaattga taaagatagc tttattggta agaaatatat aaaaggaacg aaagagttag
    13261 aggttacatt aaaagcaaaa gatgaacttt atacaatatt aaaagatgat gaaagtataa
    13321 agtcagaagt taagattaaa aatatagagt accctgttaa aaaaggtgat actatgggta
    13381 ttataaaata ttatactaat tcaggtgact tacttggaag tgtagatata gtaagtaata
    13441 atagtataga taacgtatca ttaaaaacaa aattaaaaat gaaatttgca aattaaattc
    13501 aaaaaatggt ataaaataca caaaaaaata taaaaataga aaatatggaa aaaatattgt
    13561 tatttcttca cctctgtgat atactactag aggtgaaaaa ataaaataaa tcacacacag
    13621 cattgggatc cttaaaaggt gcctattaca tggtttttta gggagaagat caagctggag
    13681 gtataaaacc aggaggtaag atatgtcagt aatatcaatg aaacaattat tagaagcagg
    13741 tgttcacttt ggtcaccaaa caagaagatg gaaccctaaa atggctaagt atatattcac
    13801 agagagaaat ggaatatata taatcgactt acaaaaaaca gttaaaaaag ttgaagaagc
    13861 ttacaagttc actaaagaag tagctgaaac aggaaagcca atcttattcg taggaactaa
    13921 aaaacaagct caagatgcga taaaagatga agctgaaaga tgtggaatgt actttgttaa
    13981 tgaaagatgg ttaggtggaa tgttaacaaa ccacaaaact ataaaaacaa gaataaataa
    14041 attaagagaa ttagaaaaaa tggaagaaga aggcgtattt aatgttcttc ctaaaaaaga
    14101 agttataaaa ttaagagctg aaaaagaaaa actagaaaaa tacttaggtg gaataaaaga
    14161 catgcctgaa ttaccaggtg caatgttcgt agttgaccca agaaaagaaa acatagctat
    14221 tcaagaagct catagattag gtataccagt agttggtata gttgatacta actgtgaccc
    14281 agaacaatta gactttgcta taccaggaaa tgatgacgct ataagagccg taaaattaat
    14341 aactggtgct atggcaacag ctgtaattga aggtagacaa ggtgctgagg aagaagtagc
    14401 tgaagaccaa gaataataat ttgacaatta aatggtaagg gtcgccctta ccatttataa
    14461 tatggaatag aataagtagg aggaaactta aaaaatggct aatataactg ctcaaatggt
    14521 aaaagagtta agagaaagta caggcgcagg aatgatggac tgcaagaaag ctttacaaga
    14581 agctgaagga aacatggaaa aagcagtaga tttattaaga gaaaaaggat tatcaaaagc
    14641 tgctaaaaaa gctggtagag ttgctgctga aggtctagtt gcaatagaaa tgaatgatga
    14701 taatacagtt gcttctatgg tagaagttaa ctcagagaca gatttcgttg ctaaaaacga
    14761 agacttcaaa gtgttcgtta aagatgctgc ttgtatggca ttagctactg ataaagaaga
    14821 tatagcttct ttattaggtg aaactcataa agaaggaata actttacaag aagttttaaa
    14881 taatagagtt gcaaaaatag gtgagaaatt agactttaga agatttgcta aagttgtaac
    14941 taatggacaa gttgctggat acattcacgg tggtggaaaa ataggtgttc ttgttgaaat
    15001 ggaaacagaa gctagagatg ctaaagtttt agaattaggt aaagatgtag ctatgcaagt
    15061 tgctgctatg aatcctaagt atgtttcaag agatgaagta gatgctgaat acatagcaca
    15121 tgaaacagaa gtattaactc aacaagcttt aaatgaagga aaaccagcta atatagttga
    15181 aaaaatggtt aaaggaagat tagaaaaaga attaaaagag gtttgtttat tagagcaaac
    15241 tttcgttaaa aatccagata taactgttaa acaattagtt gctgatgttg ctaaggcagt
    15301 aggttctgat ataaaagttg ttaaagttgt gagattcgaa gttggtgaag gtatacaaaa
    15361 gagagaagaa aactttgcag aagaagttgc taagcaactt aagtaataac caaagagaga
    15421 acacgcaggt gttctctttt ttaaaaaata gaaaaaattg gatatgaagg attgaaatag
    15481 gaggtttctg atggataaac caatgtacaa aagggtattg ttaaaattaa gtggagaagc
    15541 attagcagga gaaagaggtt ttggtataaa taatgatgta gttaatgata ttgctattgc
    15601 tataaaaaaa atacaagaaa taggtgttga agtagctgta gtagtaggtg gaggaaactt
    15661 ctggagaggc agaaccagtg aaggaatgga cagaactact gctgactata taggaatgtt
    15721 agctacagtt atgaatgcaa tggcgctaca ggatgcacta gaaaatatag atgttgctac
    15781 aagagttcaa actgctatag acatgagaca aattgcagaa ccatatataa gaagaagagc
    15841 agttagacac ttagaaaagg aaagagttgt tatatttggt gcaggaacag gaaaccctta
    15901 ctttacaaca gatacaactg ctgctttacg tgctgctgag atggaagcag aagttatatt
    15961 gttagctaaa aatgttgatg cagtatatga caaggatcca aaagtacatg cagatgctaa
    16021 gaaatttaca gaattaagtt acatggaagt tatacagaaa gaattaaaag taatggattc
    16081 tacagctaca tctttatgta tggataataa aattccaata aaggtatttg agcttacaac
    16141 agagaatata ataagagcag taaaaggcga aaatatagga actactgtaa aataatgaag
    16201 aatgaaggag gagtaaaatt atgaaactgg aaatacataa gcaattagaa gagaaaatga
    16261 atgggacaat tgatgcttta aagtttgaat ttggaactat aagagcagga agagctaatg
    16321 cacaaatgct agacaaaata agagttgatt attatggaac tccaactcca ataaatcaaa
    16381 taggtgctat atctgtacca gaaccaagaa tattaatgat atctccttgg gataaatctg
    16441 ctatgcatga aatagaaaaa gcgatagcta actcagactt aggattaaat ccatcaaatg
    16501 atggtgaggt tataagactt tctgtacctg ctttaacaga agaaagaaga aaagaattag
    16561 ctaaaaaagc tagtaaagct gcagaagaat ttaaagttag aataagaaat gaaagaagag
    16621 acgccaatga gaaaattaaa aagatggaaa aaggcggaga attaactgaa gatgaattaa
    16681 aaaaagcaca agatgaagtt caaaaaatga ctgataaatt tataaaagaa atagatacat
    16741 tattatctaa aaaagaaaag gacataatgg aggtataata ttgaaaaaat attataacct
    16801 tttaggtctt catgtagatg aggtaaaaca atattttgat gagcaaaata taaattatac
    16861 cgtaaaatct attgaaggta aaaaggataa agaaaaactt gtagttccta gagttataaa
    16921 aatatctgag ataggtgata gtgtagaact gataatcaca tacttttctg actccttagt
    16981 ttaaggagtc agaacaatat tggaggcaaa atatgaataa caacattata tatgatatag
    17041 acttaaataa catacctact catattgcta ttataatgga tggaaatgga agatgggcaa
    17101 aagctagatt tcttccaaga actgcaggac ataaggctgg agtagaaact ataagagata
    17161 tagtaaaaga atgctcaaaa ttaggtgtta agcacttaac attatatgca ttttcaacag
    17221 aaaactggaa aagaccgaaa cttgaagttg acacacttat gaacttatta tctacatact
    17281 taagaaatga aatagctgaa cttcaccaaa acaatgtaaa agtaactgct ataggagata
    17341 taagtgcttt accaaaaact tgtataagag aattaaactc agctaaggaa ctgacaaaaa
    17401 acaatacagg agttaactta aatttagcat taaactacgg aagtagagca gatataaaaa
    17461 atgctcttat agatatagta aaaaattgtg aaagtggtaa aatagatata aacaatattg
    17521 atgaagatat tataaaaaac tatttaagca caaagtctat accagatcca gacttggtaa
    17581 taagaacaag tggtgaacaa agattgagta actttttact ttgggaagtt gcttattctg
    17641 agttttactt tactgatata cattggcctg attttaaaaa agaagaacta cagaaggcta
    17701 tatacgtata tcaaagaagg gatagaagat ttggaggact taagtaagga gattatttta
    17761 tgcttacaag aattattgct tcactagctc tggttccatt atttctattt gttgtatatg
    17821 gaggtattcc tctatatata gctgagacag caatagttta tattgcattg catgagtttt
    17881 ataaggcatt taaaataaaa gatgtacatc caatattcat tataggatat ttattttcta
    17941 tttatttagc tgtaaaaaat atttttaatt taccattaga atatacttat gcagtaattt
    18001 ttattttatt tttagcaagt ataatttata tgttgatggg caaaaacaat gttatagatg
    18061 tttcgattac gtttttagga gttttttata taggagtatt tttagatttt ataattataa
    18121 ctataaatgg atttgaaaaa ggtagtatat atgtatggct tatatttgtg atatctttta
    18181 tgacagatat atttgcatat tttagtggtt atttacttgg aaaacataag ctaattccta
    18241 aagttagtcc taaaaagact atagaaggag ctattggagg aataatagga agtacacttt
    18301 gttgtatttt atttggatat ttatttggca ttgatttgct tcaattagcg ataataggaa
    18361 gtataggaag tgtaatagct caattaggag atttatttgc atcttctata aagagatatg
    18421 tagggataaa ggattatgga aagataatac ctggtcatgg gggtatatta gatagatttg
    18481 atagtgttat attagttgca ccttttgtat atagtgcgat taaatttttt attagataaa
    18541 gattggttgg atttaaaaga gcaatctata attgctagta atgaaaatag ctatgtaact
    18601 tttaaatgca gagatattct gcagttatta gttacatggc tattttcatt gttcaaaaat
    18661 ttagaagacc ttggggaatt gtataagaat aggtattact ttataatagt atactatatt
    18721 tgtagtgagt atatttttta tactataact atcattaaaa aatatttttt tgtttaataa
    18781 tttcgatatt tttagctata ttatatattt ataatatata tgta
//
LOCUS       DAGSRM010000077        18729 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.077, whole genome shotgun sequence.
ACCESSION   DAGSRM010000077 DAGSRM010000000
VERSION     DAGSRM010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18729
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.077"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(8..1066)
                     /locus_tag="MJE33_002746"
     CDS             complement(8..1066)
                     /locus_tag="MJE33_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBY9648674.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSSQNTKYSKTSYYLGTVNE
                     VTVFNVKESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVRVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSKIQKLLPLIDYKNIILDE
                     KNKSIKLTKENMKIDLGAIAKGYAADKIVAYLKSEDVKKGLVNLGGNIFTLENGKNDK
                     PFKIGIQDPTSKNGESIGNIETTNKSVVTSGIYERFIEKDGKIYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMNLINKIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKSFSIEKLNKQ"
     gene            1133..1504
                     /locus_tag="MJE33_002747"
     CDS             1133..1504
                     /locus_tag="MJE33_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBY9648675.1"
                     /translation="MKKKDILLILGILVVITACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIESRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            1541..2215
                     /locus_tag="MJE33_002748"
     CDS             1541..2215
                     /locus_tag="MJE33_002748"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBY9648676.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            2464..3303
                     /locus_tag="MJE33_002749"
     CDS             2464..3303
                     /locus_tag="MJE33_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBY9648677.1"
                     /translation="MNFKKLSNAKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDSNDIVTLDENTKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPVNKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     ILK"
     gene            3547..3643
                     /locus_tag="MJE33_002750"
     tRNA            3547..3643
                     /locus_tag="MJE33_002750"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3581..3583,aa:Sec,seq:tca)
     gene            3794..4501
                     /locus_tag="MJE33_002751"
     CDS             3794..4501
                     /locus_tag="MJE33_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HBY9648678.1"
                     /translation="MENNFTTRTSFLVGDDGIKKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NKDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIVNELLGQ"
     gene            complement(4643..5479)
                     /locus_tag="MJE33_002752"
     CDS             complement(4643..5479)
                     /locus_tag="MJE33_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBY9648679.1"
                     /translation="MRNKIFLSILIIISLLIGCDKKSLLSIHMIDVGQGDSILVQTPT
                     NKNILIDGGDEDSENIIISYLRQKRIKTIDIIIATHPDSDHIGSLDNIIKKFNVNSIY
                     MPEQSTDSKAYQNLINSCTDKNLSIQHLYKNDVLNIDNNINIYVLSPSYIQEESNLNS
                     IVFKLTFNDNSFLFMGDAEEENEKEILHSFKLNNINFIKIGHHGSNSSSSLEFIKKIS
                     PDIAAISCGYKNQYGHPHREVINNLKQNHVSIYRTDRIGDIVFYSDGEIIFTKYNYEI
                     DT"
     gene            5667..6572
                     /locus_tag="MJE33_002753"
     CDS             5667..6572
                     /locus_tag="MJE33_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HBY9648680.1"
                     /translation="MFFKDIELNSKKELDPYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPGRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRYEAKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            6581..7765
                     /locus_tag="MJE33_002754"
     CDS             6581..7765
                     /locus_tag="MJE33_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY9648681.1"
                     /translation="MEIRYAKEEEIESIKDIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNSKVYNTSYVVGVSTLPEGRGAGYMNKVMKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSNLSQIDDLI
                     QIDRTFLNEVNGNVLKDEHYYENLFKEIQSEDGFLYIHEGNEKDGYIVYFLQEDKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPTIDKIRFILDNPKDSDIKIKPFMMG
                     RVINVKKFIEDIDIEKDINSSFNLLIEDKFIDENNGLFKISIQNKKVSVEQLDKKGAE
                     KPQEDFDIKLDINTLTQLSFSYIDVNEAIFLNDIKDVSEETLETLNCIFSKKNNYINE
                     YI"
     gene            7880..9181
                     /locus_tag="MJE33_002755"
     CDS             7880..9181
                     /locus_tag="MJE33_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HBY9648682.1"
                     /translation="MGVIIIQIRRPLLIILMFVVAISFIITNNTKNDEELNDKIITIQ
                     GVVKGKIEKKRYNQYKVGVFLINDYTKNKNLKIGQKVNITGKFKSLDKMKYDDFDYGR
                     YIKSTGYKGIVYINNYKIIDKNKLYSLIGEIKFYISKTYRYLYKKNSDFINSILLAEV
                     ENLTEEQKEIFTRTGTSHVISISGLHTGILCVIISFLLRGINKLYKLLILSIFITLYC
                     IMVGASPSIIRSIAFVMIFYLSIFIDRKKDGISALSLIGIILIMNNPYVIYNVSFQLS
                     FLATLSILIFYNKINSIIKLSMVSLTISSNILTLPIIYYTFKGIPLLSIIGNLIIVPF
                     VGVIMYLSIASLIVFKVSVVIAKIISFFNSTFIESIFFLLEKISNLSFAYINIENPKF
                     YIVVIYYIGVFFYIFYIEGKEIKEQENELQGYYKECKREKL"
     gene            9135..10178
                     /gene="holA"
                     /locus_tag="MJE33_002756"
     CDS             9135..10178
                     /gene="holA"
                     /locus_tag="MJE33_002756"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBY9648683.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSLVKKIGNNGIVFDCDKLSDMDLFKWIKKSFALN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(10365..10631)
                     /gene="rpsT"
                     /locus_tag="MJE33_002757"
     CDS             complement(10365..10631)
                     /gene="rpsT"
                     /locus_tag="MJE33_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBY9648684.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
     gene            11227..11748
                     /locus_tag="MJE33_002758"
     CDS             11227..11748
                     /locus_tag="MJE33_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY9648685.1"
                     /translation="MDIAKLILNPARLRILQYIRLYGSVRTSDIVKYLNDIPRATVYH
                     HVKILEENNMIEVIKENRVRGTIEKVYALKEYTTSMEGETFVALSTAFYVGLMQEMNE
                     YFSRKEQDHKKDNVFFSSALLYMSDNEYENLLKSIADLLKPYIEQKPKSNLKLRKLSI
                     ISSPPVADNDKTK"
     gene            11782..12558
                     /locus_tag="MJE33_002759"
     CDS             11782..12558
                     /locus_tag="MJE33_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBY9648686.1"
                     /translation="MKFHEFGNKNRPHIMLIHGGGNAWWNYLRQAEVLSNKYHVILPT
                     LDGHGEEYETTYISTEDTADKLISYIDENCSGHLFALCGVSLGGQIVMELLSRKPNIT
                     KKAIIDGSICYPRPLMARFCIAGIRFFGGLLFSEKACRFQIAMMPKLLPENMQYPDEI
                     KAYYMTDMPHVRKETFYNMYRTYMMNYTLKENVKKTTAQVMYWYGEKEMKCVKKSAKL
                     FQSYVPSCEIYEAKSYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            12900..13853
                     /locus_tag="MJE33_002760"
     CDS             12900..13853
                     /locus_tag="MJE33_002760"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HBY9648687.1"
                     /translation="MISVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDRQGAEIMNKDIGKYITLESNLMKFDDDESREEMISYLKEELVDIFGQDKNKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            13871..14890
                     /locus_tag="MJE33_002761"
     CDS             13871..14890
                     /locus_tag="MJE33_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HBY9648688.1"
                     /translation="MFKKCIKVVTLTFILVCILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTENKKNNKNKETSKENKEESKEGNTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNTTSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDEY
                     FKQKN"
     gene            14921..15172
                     /locus_tag="MJE33_002762"
     CDS             14921..15172
                     /locus_tag="MJE33_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648689.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRILFLITMIICTSVCVFIVDSNA
                     KSMLGMKQYDFPSIKFDITKFDFDIPNFDIHEIIKKFNK"
     gene            15454..17259
                     /gene="lepA"
                     /locus_tag="MJE33_002763"
     CDS             15454..17259
                     /gene="lepA"
                     /locus_tag="MJE33_002763"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBY9648690.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDDRRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            17408..>18729
                     /locus_tag="MJE33_002764"
     CDS             17408..>18729
                     /locus_tag="MJE33_002764"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBY9648691.1"
                     /translation="MINIEIPKKVDYIIKELEKNGYEAYIVGGCVRDCLLERVPNDWD
                     ITTSARPEVVVELFEKTIPTGIQHGTVTVMIEHEPFEVTTYRIDGNYSDGRHPDSIEF
                     TNNIVKDLSRRDFTINSIAYNSKTGLVDPFNGYEDIQNKYIRCVGNPVDRFEEDALRM
                     LRAVRFSAQLNFKIAEGTKQSIHKKADLIKNVSIERIQTEFNKILVSDSSKLNLLKST
                     GLLKFIIPEICELEDVTQHNPYHIYDVQKHTLIATEAIEDELYLKLTMLFHDLGKKVT
                     KTTDKNGVDHFYTHSRESVKIAKKILKRLKYDNYTINKVLILIQYHDYRIEPKRKIIK
                     KLLNKLEDVELFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECFNLK
                     DLAINGKDLISIGIKPGKDIGHILNKMLEIVINNPELNEKEILKEKAL"
BASE COUNT         7403 a         1999 c         3074 g         6253 t
ORIGIN      
        1 tttaaactta ttgtttattt aatttttcaa tagaaaaaga tttatctgta agtttaaaat
       61 tatcttttat acccttagtt aagtatactt tcttatcctt agttatgaat atagcatcta
      121 cattatcaat tttattaatt aaattcatac ctttttcaag accaagacca aatgtagaag
      181 tggataaggc atcacaatta atagatttat cagagattat tgtaacacta cttaaattgt
      241 tttcaaaagg ataccctgta aatggattta acatatgatg atatattttt ccgtcttttt
      301 cgataaatct ttcataaatt ccagaagtaa caacagactt atttgtagtt tctatgtttc
      361 ctatggattc accattttta ctagttgggt cttgaatacc tattttaaaa ggtttatcat
      421 ttttaccatt ttctaaagta aatatatttc ctcctaagtt tactaaacct ttttttacat
      481 cctctgattt taaataggct acaattttat cagcagcata tcctttagct atggcaccta
      541 aatcaatttt catattttct ttggttaatt ttatagattt atttttttca tccaatatga
      601 tgttcttata atcaataaga ggcagaagtt tttgtatctt agaatcactt ggaactttag
      661 ctttatctgt tccaatagcc cataaatttg ataatggacc aattgttatg tcaaaatatc
      721 catctgataa tttggaatat tgtatagctt cttttacgac gaaaaatgta tcatcagaaa
      781 ctctgacaaa ttttttacca gcattatcat ttattttaga aacatcactt cctggtatat
      841 gagtgctcat tttgttgtca atatatctta aaattgaatc acattcattt aagattttat
      901 cacttttgga ttcttttaca ttaaatacag taacttcgtt tactgtacca aggtagtagc
      961 tagttttact atatttagta ttctggcttg aactcctaaa aaatgaaaat aaaattaatg
     1021 ctaaaatcaa tatcacaaaa ataaatgtga tttttttctt attcatggta tattcctcct
     1081 atgaaattgt gttattattt tattataaca taatttcata ggaggacaaa aaatgaagaa
     1141 aaaagacatt cttcttattt taggcatttt ggttgttatt acagcatgtt atgggataat
     1201 aaatgttata aattctaaaa atgctggaaa tattgagatt tatgtagata ataaattgta
     1261 taaaactgtt tcaataaatg ccaaagaaga atttaaaata gagagtagag gggggtataa
     1321 catagtaaag attcatgata aaggggttga gatagtagat gcttcatgcc ctgataaggt
     1381 ttgcgtacat actggattta taaacaagcc aagtcaaagc atagtttgta tacctaatag
     1441 agtgagcata aaaataaata caaatgaaaa gaatgataat caagaagata taatttctaa
     1501 ataaaataat aaaaataatt gaaaggaatt ggtgataatt atggttaact taggaaacga
     1561 ttgggatgaa ttattgaaag aagaatttga aaaagattac tacttaaact taagaaaatt
     1621 tcttatagaa gaatataaaa cacgccaaat tttcccaaat atgcacaata tatatgaagc
     1681 tttaaaacac acttcataca aagacacaaa agtcttaata ttaggacaag acccttatca
     1741 tggagataat caagctcatg gtttagcttt ttcagttcaa ccacaagtga aaactccacc
     1801 atctttatta aatatgtata aagaattaaa agatgattta ggttgtttta tacctaataa
     1861 tggatattta atgccttggg cagaccaagg agtgttactt ttaaatactg ctcttactgt
     1921 tagagcgcat gaagctaatt ctcataagaa caaaggatgg gaaattttca ctgatagagt
     1981 aatatctatt ttaagtgagc gtgaagaccc agttattttt gtactatggg gaagtaatgc
     2041 acgtaaaaaa gtagagttga ttgatacaag taaacattat attttggaag cacctcatcc
     2101 aagcccattg tcagcaagta aaggcttttt tggatgtaaa cacttctcta agataaatga
     2161 aattttgaaa aagctaggaa aagaacctat taattggcag atagaaaata tataaaagta
     2221 aaaggtaaca aaatgataac aaaacaacaa taaacaatga cattgttgta actaatagaa
     2281 atatgcttaa atagttgaaa atactatgta tgcatatttc tatttttttg tcaagttgac
     2341 aactcttgaa aaaatagtta caatttagtt acacaaaaaa cacaaataaa aaaagaaact
     2401 tacaaagcag aattttatga agcaaaatat atagtgtaaa aaatcttagg gaggaaattt
     2461 tatatgaatt ttaaaaaatt gagtaatgct aaagtctcgg taatggcagt tgttttatca
     2521 gttggaatat tatcgggata ctctgcgctt aataaacaag taacattagt agtaagggga
     2581 gaagaaagaa ctatatcaac atttagttct aatgtaggag accttttaga atctcaaaac
     2641 attagctatg attcaaatga tatagtaact ttagatgaaa atacaaagtt atctaatgga
     2701 gataaaatag aagtaattga tgtaaaggaa aaaacagttg tagaaactaa agacattcca
     2761 tttgaagtta cagttgttga agatggctct ttattaaagg gtgattctaa ggttaaagaa
     2821 gaaggaaagt ctggtaaaag tgaattaata tataaagaaa cttatcacaa tggaaagaaa
     2881 gtagaaaagg aatttgtgaa aaaggttgtt aaagctgagc cagtaaataa ggttgtaagc
     2941 aaagggacaa aggtagaaat gcaagtagcc tcatcaaggg gagaaagttc aagaagaatg
     3001 gcttcatcat catctagtag cagtagtagt aacaatggta aaaatatgag agtggtagca
     3061 actgcatata ctggtcacag tataactgca acaggaacaa aacctaagtg gggaacaata
     3121 gctgtagacc ctaaggttat accatatgga actaaagttt atatacctca atttgacatg
     3181 gtgtttactg cagaagactg tggtggagca ataaaaggaa ataaaattga tatctacatg
     3241 aatgattcaa aaacagttta taactgggga agaaaaacta tagatatacg tatattaaaa
     3301 tagttaaata atttatattt ttactggaaa ttatattttg attatataga gaagaaactc
     3361 tttatagatt ctaaaaatat aatttccagt tttttatttt ggatatgtaa taaaacaggt
     3421 aaattttaac catgtataga taaaagctat ttaaattata atttactatc atataaatct
     3481 ataaaaattg ttgcaaagag tcaaaatata tgatattata gatttcgttg agaaggattt
     3541 cattatggga gtggatgggt gccggtgtgc cctctagtct tcaaaactag tatgaggggt
     3601 taagagcctc ttaggtgggt tcgattccca cgcattcccg ccaatatgta aaaggtatct
     3661 tttaattaaa agataccttt ttaaatttat tttaagataa tttattttta ataatatata
     3721 atgttttgta ttatataatg taaattttct aatactatta tatatttaga taaaaatcta
     3781 ggagtgtgga aagatggaaa ataattttac aacgagaact agttttctag ttggagatga
     3841 tgggataaaa aaacttaata attcaaatat aatagtattt ggagttggag gagttggtag
     3901 ttttactgtt gaagctttag caagagctgg agtaggcaat attactattg tagattttga
     3961 tgatgtagat ataaccaata taaatagaca aataccagcc ctacattcaa ctgtaggaag
     4021 atacaaagtt gatgtcatgg aagaaagaat attagatata aatcctaata tcaatataaa
     4081 aaaaataaga agtctttata ataaagatac tagtgacgaa atactaactg aaagatatga
     4141 ttatgttgtg gatgcgatag acatggtaag ttccaaaata catctaattg aaacttgtga
     4201 aaaaaaagga ttgaaaataa taagttctat gggtatggga aataaattag acccaactaa
     4261 aatagtcgtt acagatatac acaaaacaag tacttgtcct ctagctaaag ttatgaggaa
     4321 agaacttagg gatagaggta tcaaaaagtt aaaagtagta tattctactg aacaaccaat
     4381 agaacttaag aagaaagtaa tgaatggtag aaaagtaaca cctggtagtg tatcatttgt
     4441 cccatctgta ggaggtttaa taatcgcatc tgtaatagta aatgaactat taggacaata
     4501 agttttaaat attttaattt ataaatttta tataaaaatt tgaaggctag aagcatgaat
     4561 acaaagcaat tttatattca tacttttagc ctaattattt tatattacct tattttatgt
     4621 agttgtagtt ttatttttta gattatgtat ctatttcata attatatttt gtaaatataa
     4681 tttctccatc actataaaaa acaatatctc ctattctatc tgttctataa attgatacat
     4741 ggttttgttt tagattatta ataacctccc tatgtggatg accatattga tttttatatc
     4801 cacatgatat tgcagctata tctggactaa ttttttttat aaattctaaa gatgaagaag
     4861 aattacttcc atgatgacct atttttataa aattaatatt atttaattta aaagaatgta
     4921 aaatttcttt ttcattttct tcttcagcat cacccataaa taaaaaagaa ttatcattaa
     4981 aagttaattt gaaaactata gaatttaaat tagattcttc ttgtatatat gatggactta
     5041 atacatatat atttatattg ttatctatgt ttaaaacatc atttttatag agatgctgaa
     5101 tagataagtt tttatccgta caagaattta ttagattttg ataagcctta ctatcagttg
     5161 attgttcagg catatatatg gagtttacat taaatttttt tataatatta tcaagacttc
     5221 ctatgtggtc tgaatctgga tgagttgcta ttataatatc aatagttttt attctttttt
     5281 gtctaagata actaataatt atattttcgc tatcttcatc acctccatca attaatatat
     5341 tcttatttgt gggtgtttga actaaaatac tatcaccttg tccaacatca atcatatgta
     5401 tagatagtag agattttttg tcacagccga ttaaaagtga tattataata agtattgata
     5461 aaaaaatttt atttctcaat aaaaaatcac tccattcatt acatgtttat ataaatttat
     5521 gatgggtaat ataagatata tatgacttat tatattttat attttaaaca atataaatga
     5581 aattaatacc ataataaaat tggagttttt tatatataca aaataaaatt tttaaaatta
     5641 agcaataaaa aaggtaggag gattttatgt ttttcaaaga tattgaacta aattcaaaaa
     5701 aagagttaga cccatatttt gatttagtag attatgaagc ttgtgagtac tgttttagta
     5761 ctttatatat gtggcaacat gtttataaga caggatatta tataggtgaa gattttgccg
     5821 ttttagttgg ggaatacgaa ggagatagct tctcaatatt accacttgct aaaaaagata
     5881 aacttccaga agttgtagat tttgtccttg aatatttttc taaaaataat aaaaaaatat
     5941 acttaagagg tattactact gaagtagtag agtttttaaa agaaaaatat ccaggtagat
     6001 ttgagtatat agaagaaaga gatttatttg actatattta tgatgcagaa agtttaagaa
     6061 ctctagctgg taaaaagaat caaaagaaga gaaaccatat aaactatttc ttaaaagagt
     6121 atgcaggtag atatgaagct aaattattag ataaagaaaa ctttgatgag tgtttagtac
     6181 taatgaagga atgggaaagt aataaggaag aaaataatga atttgatgaa agtatggatg
     6241 atgaattaat aggtatcaaa aagatattta accattatga catattaaaa gataaggtaa
     6301 aagtgtttgg tgtttatgta gatggaaaat tagaagcttt tagtatagga gagcttttaa
     6361 atccaaatat ggcattgatt catatagaaa aagctaatcc cgatataaga ggtctgtatc
     6421 catttataaa tcaacaattt ttggtaagtg aatttaagga cgttgagttt gtaaatagag
     6481 aagaagattt aggtattgaa ggattaagaa aagctaaact ttcttatcat ccttgcagat
     6541 ttgttgaaaa gtacagtgtt agggaggctt aatagactat atggagatta ggtatgctaa
     6601 agaagaagag atagaaagta taaaagatat ttggagttat tgttttaacg atacggagag
     6661 ttttatgaag tactatttta atgataaata taaatctgaa aatactgtag tagctttaga
     6721 tgaaggaaaa attatttctt ctcttcagct aaatcagtat aagttattac taaatagcaa
     6781 ggtatataat acatcatatg tggtaggagt ttctacactt ccagagggaa gaggagctgg
     6841 atatatgaac aaagtaatga aatttacttt aaacgaattg tataaaaaag ggcaattagt
     6901 ttctatattg atgcctattg actatagact ttatagaaga tttggatatg agcattgtta
     6961 cgaccagatt gaatatacta ttaacacaga tgatttaaaa aattttaagt caagtggaaa
     7021 aatgataaag tctaatttat cacaaattga tgatttgata caaatagata gaactttttt
     7081 aaatgaggtt aatggtaatg tgttaaaaga tgaacattat tatgaaaact tatttaaaga
     7141 gattcaaagt gaagatggat ttttatacat tcatgaagga aatgaaaaag atggatatat
     7201 agtttatttt cttcaggaag ataaaatgtt tgtcagagag ttattttata aaaatataga
     7261 tgcccttaaa tcaatgctta aatttatata taatcacaat actcaatgta agatagttac
     7321 tatatctact cctacaatag ataagataag gtttatatta gacaacccta aagatagtga
     7381 tataaaaata aaacctttta tgatgggaag agttataaat gtaaagaagt ttattgaaga
     7441 tatagatatt gagaaggata ttaatagctc atttaattta ttaatagaag acaagtttat
     7501 agatgaaaat aatggtctat ttaaaatttc tatacagaat aagaaggtat ctgtagagca
     7561 gttagataaa aaaggtgcag aaaagcctca agaagatttt gatattaaat tagatataaa
     7621 taccttaaca cagttatcat tttcttatat agatgtaaat gaagctattt tcttaaatga
     7681 tataaaagat gtttcagaag aaacactaga aacacttaat tgtatttttt ctaaaaaaaa
     7741 taactatata aatgaatata tataggtaga gtacgttaaa agattttaaa aatcttttga
     7801 cgtatttttt tgtttagata acaatttatc ttgtttaaaa attttctatt tgaaaataat
     7861 ctaaataata tacttttata tgggagtgat tataattcaa ataagaagac cattattaat
     7921 tatattaatg tttgtggtag ctatatcatt tatcattaca aataacacaa aaaacgacga
     7981 agaacttaat gataagataa taactataca aggagttgtc aaaggaaaaa ttgaaaagaa
     8041 aagatacaat cagtacaagg taggggtttt tctaataaat gattatacaa aaaataaaaa
     8101 tttgaagatt ggtcagaaag ttaatattac aggtaaattc aaaagtttag ataaaatgaa
     8161 gtatgatgat tttgattatg gaaggtatat aaaaagtact ggatataaag gtatagtgta
     8221 tataaataac tataaaatta ttgataaaaa taaactttac tctttaatag gggaaataaa
     8281 gttttatatt agtaaaactt ataggtactt atataagaaa aactcagatt ttataaattc
     8341 tatactatta gcagaagttg aaaatttgac tgaggaacaa aaagaaattt ttaccagaac
     8401 tggaactagt catgtaatat ctatatcagg tcttcataca ggaatattat gtgtaattat
     8461 atcctttcta ttaagaggta taaataaatt gtataaatta ttaatactgt cgatttttat
     8521 aactttatat tgtataatgg taggtgcgtc tccatccata attaggtcta tagcatttgt
     8581 tatgattttt tatttatcaa tatttattga tagaaagaaa gatggaatat cggcattgtc
     8641 tctaattggg ataattttaa ttatgaataa cccctatgtt atatataatg taagttttca
     8701 attgtctttt ttagctacac tttctatatt aatattctat aataaaataa atagtattat
     8761 aaaattaagt atggtatcat tgacaatatc atcaaatata ttaactttac ctataattta
     8821 ttatacattt aaaggtatac cattgctttc aattatagga aatttaatta tagtgccatt
     8881 tgtgggagta ataatgtatt taagtattgc aagtttaatc gtatttaaag taagtgtagt
     8941 aatagctaaa attatatctt tttttaatag tacatttata gaaagcatat tttttttact
     9001 agaaaaaatc agcaatttaa gttttgcata cattaatata gaaaatccta aattttatat
     9061 tgttgttatt tattacatag gagtgttttt ttacatattt tacatagaag gaaaagagat
     9121 aaaggagcaa gagaatgaat tacaaggata ttataaggaa tgtaaaagag aaaaactttg
     9181 aaaaaatgta tcttttttat ggaagagagt attatttaat tgagaatgca ataaaagcat
     9241 ttaaagatag tctaaacgag ggaatgcttg attttaattt agatataata gatgggaagg
     9301 aaatagtctt aaatcagctt ataagctcta tagaaacact tccatttatg gatgatagga
     9361 agatagtaat aataaaagac tttgaactat taaaaggaaa gaaaaagaat tttacagata
     9421 gtgatgagaa atacttaata gagcatttag acaatattcc agacaccaca actatagttt
     9481 ttgttgtata tggagatgta gacaaaagaa aatcattagt taagaaaata ggaaacaatg
     9541 gaattgtttt tgattgtgat aagctttctg atatggattt gtttaaatgg ataaaaaaat
     9601 cttttgcttt aaatgatgtt ataatagata attctcaaat catgtatttt attgaacaag
     9661 aaggatacag agataagagt agtgaaaaga cattatctga tttagaaaat gaaataaata
     9721 aaataagttc atttgttggt aaagggaata atgtaaccaa tgatgtaatt gataaattat
     9781 ctcaaaaaaa agttgagaat gatatattca aattaatcga ttatattgga gaacaaaatg
     9841 cttctaatgc aatgaaaata ttaaatgata tgatacaaga aggcgaatct gtattaggta
     9901 tattttctat gatagcaaga caatttaaaa taattatgca agtaagacag ttacaacttg
     9961 atggatattc aacaaaactt atagctgata aattaaaaat gcatcaattt gttgtaggaa
    10021 aagcattaaa gcaaactaaa aatttttctg atgatataat tgtggaaata ttaaactata
    10081 tattagaaag tgattacaaa ataaagacag gtcttataag agatacttta gcagttgaaa
    10141 tgttggttag tagatactgt aaaagagaag caatttaaaa aatctatata ttaataaaaa
    10201 ataaatagca agatatgtta tagttcagag tagatgtaag ctaaattaag tttgatatac
    10261 aatcaactat aatatgtcta actatttatt ttttattgta taaatttaca aaataaaaaa
    10321 cccctacata ggggttatta tatataagaa atataaactg gtaattaagc gttcataccg
    10381 tttaattttt gagctaattt agctactttt ctagctgcag cattcttatg tatagttcct
    10441 ttagaagcta cttggtatat tcttttttga gcgtattgga acttagcaac agcatcctct
    10501 ctgtttccag ctgcaacagc atcttcgaat cttcttattg cagtttttat ttgagatttt
    10561 ctaactctat ttaaagcagt tttcttttcg ataacactta ttcttttctt agctgatttt
    10621 atatttgcca ccgtgttcac ctccctgtat ttttttatca tattcatcgt caacatatta
    10681 gattttaaca gaaataaatc taaaaatcaa ggattattat ttatatattt taataatgtt
    10741 ttttattttg attttttaga ctgtaaattg tgtataaata atttgcaata tcttgttttt
    10801 aaattggtat attttgtgtt ttaaatattt tgacatatat aattaatatt tcattttgta
    10861 acaaatattt gagataatac aaactattaa catacatttt gatagtattt ccaataatta
    10921 taagataata caagaaatta ctcatttttg taaatttaat gaaaaattat aaaaatataa
    10981 atttatatgc ttaattaaga taatttttcc aataatcaag ataatttttt tcattagtat
    11041 gtatgataga tgcatcaaac aatataacta atatgttaga aggttatatg taattaatgt
    11101 cagtgtttat atagaatagc atttgtagca tttattatag ttgtaattac tatttatatc
    11161 atagacttga atagtctaaa aaaatagact ataatatttt tatagactaa agaaagggct
    11221 gataagatgg acatagcaaa actaatttta aatcctgcaa ggcttcgtat tttacaatat
    11281 attcgacttt atggcagtgt tcgtacatct gatattgtaa agtatttaaa tgatattcct
    11341 cgtgcaacag tttatcacca tgtaaaaatt ttagaagaga acaatatgat tgaagtaatt
    11401 aaagaaaatc gtgtgcgtgg tactattgaa aaggtttatg cactaaagga atatacaaca
    11461 tctatggaag gagagacctt cgttgcactt tctacagcat tttatgtagg tctgatgcag
    11521 gaaatgaatg aatactttag cagaaaagag caagaccata aaaaggataa tgtatttttc
    11581 tcttcagcat tactatatat gagtgataat gaatatgaaa atcttcttaa aagtatagct
    11641 gatttattga aaccatatat agaacaaaaa cctaaatcaa atctaaaact tagaaaactt
    11701 tcaattatat cttctccacc tgtagctgat aatgataaaa caaaataaca tgcaacccat
    11761 ttttataaaa ggaggttata tatgaaattt catgagtttg gaaataaaaa cagaccacat
    11821 attatgttga ttcatggggg agggaatgca tggtggaact atttgcgaca agcagaagtt
    11881 ttgtctaaca aatatcatgt gatacttcct acattggatg gtcatggaga agaatatgaa
    11941 acaacataca tttctacaga ggatacagca gataagttga tatcatatat agatgaaaat
    12001 tgcagtggac atcttttcgc tttgtgtggg gtatcacttg gtggtcaaat tgtcatggaa
    12061 cttttatctc gaaaacctaa tattactaaa aaagcaatta ttgatggtag tatttgctac
    12121 ccaagacctc ttatggcaag gttttgtatt gctggtattc gattttttgg aggacttttg
    12181 tttagtgaga aggcttgtcg ctttcaaatt gctatgatgc caaaattgtt acctgaaaat
    12241 atgcaatatc cagatgaaat aaaagcgtat tatatgacag atatgccaca tgtaagaaaa
    12301 gaaacattct ataatatgta tcgtacttac atgatgaact atacacttaa agagaatgtt
    12361 aaaaaaacca cagcacaagt aatgtactgg tatggagaaa aagaaatgaa gtgtgtcaaa
    12421 aaatctgcaa aactgtttca gtcttatgtg ccctcttgtg agatttatga agctaagagc
    12481 tataatcatg ggtatttatc actttatctt ccaaatgaat ggttggaaat tgcagaacca
    12541 tttttccaaa aggaataaag gtgttaggtg attattgtat aattattgct gtttttttat
    12601 atagtaaata aattaggtat aaatataaaa aattatttta gttaatatat tagtgaaagc
    12661 ttaataggaa aattataaat agattaaagg tagcacctat ataaatagtt gctacctttt
    12721 tctgtttgta aataaatgat agataagaag gtttactgac aaatcactga ctaaacccaa
    12781 ttttcttaca ttgaaagcct taattttact tgttttaaat aaatttacaa tttcttgtta
    12841 taaaatacta aattttgtta acaattataa ttagcattta atttatactg gaggtttata
    12901 tgattagtgt aagaacagat ttagctttag aagctagtga aatgtgtgaa aagtctcaag
    12961 aaggaagtag tattcctggt gtaaaaatag aaactaaaga attagaaaat tgcattgtaa
    13021 ctaaagttga agtaatagac aggcaaggcg cagagataat gaataaagat ataggaaagt
    13081 atataactct ggaaagtaat cttatgaagt ttgatgatga tgagtcaaga gaagaaatga
    13141 taagttattt aaaagaggag ttagtagata tatttggcca agacaaaaat aaaaaaaccc
    13201 tggtaattgg tcttggaaat agaaatatta cttctgatgc gttaggacca aagagtgtat
    13261 caaaaacact tgtaactaga catcttttta aaaattataa taaagactat gatgatgatt
    13321 tcactgaggt atcagcttta agcccaggag ttatgggtgt tactggaata gaaactagtg
    13381 aaatagtaaa atcattggtt gagaaggtta agccagatag agtagtagca atagatgcat
    13441 tggcttcaag aaaaatggag agagtaaact ctactataca gatatcaaca gcaggaatat
    13501 cacctggtgg aggtgttggc aatactagaa aatctctaac taaagaaact ttaggcgtgg
    13561 atgttattgc aataggcgtt ccaacggtgg ttgatgcagc aactcttaca atagatgtct
    13621 tagatatggc aatagacaat ttaatagctc aatcagaaga aacagaaagt ttttatgaaa
    13681 tgttgaaaaa acttaaagaa gaagaaaaat atcatctaat aaaagattcg ttagacccat
    13741 atgacaagaa cttaatagta acaccaaagg atatagatga tacaatcgaa aatctatcta
    13801 taataataag tgaaggctta aataggtctt tacatccagg tagattggta taaaataagg
    13861 aggagcaatt atgtttaaaa aatgcattaa agtagtaacc ttgactttta tattagtatg
    13921 cattttacca ggaaaatcat tagctttgaa tcaagatgat tttttaaagt ttttagtaaa
    13981 ttcatcttat ccagaagcta aagtcgaagg aaatgataca gaaaataaaa agaataataa
    14041 aaataaagaa acaagtaagg aaaataagga agaaagtaaa gaaggaaaca ctaaatctaa
    14101 agatgcaagt aaagttgata ataaaaaaga gagtgaaaaa gaatatataa aactttatgt
    14161 tggtaaagaa aatgttccag atattgagtc aaaaaattca gacacaactg aaactaacac
    14221 aacatctagt tcagattata aggatgattt aagagtaacc aaagaaaatc caagaatttt
    14281 gatatatcat actcatggat gtgagactta ttcaaattca cctgatggaa attatcattc
    14341 aagagataag aaaaattcag taatggaagt aggaagtgca ttaactagtg ctttagatag
    14401 caaaggatgg ggtgtagtcc atactactaa ataccatgac tatccatctt ataataactc
    14461 ttatgcaagt agcttaaaaa caatacaaag tatattacct aaatataact cagtggatat
    14521 agcgatagac ttacatagag atgctaggga tttaactaat ccagccacta aggaaaaaga
    14581 ccatttaaaa tatacaacta tgataaatgg agagagagta tcaaagttct tctttgttgt
    14641 aggaggtaaa aatacaaata gaaaacaact tagagcttta gcagaagata taactgcttt
    14701 tgctgagaaa aagtatcctg gtctagtatc tccaatagta gaaaaagact atgctagatt
    14761 caatcagttt gcagttaaga atcatatgtt agtagaaata gggaataacg caacaagtgt
    14821 agaagaatct aaggctacta caaagtattt agccgagatt ttggatgaat attttaaaca
    14881 aaaaaattag agctttaagt tataattagg agcatgctaa atgagccgat tagaaaaaaa
    14941 tatagaaagt aaaagtaaac taagaaaata caagtatatt atgagaatct tatttttgat
    15001 tactatgatt atatgtacat cagtttgtgt atttatagtg gacagtaatg caaaaagtat
    15061 gctaggaatg aaacaatatg attttccaag tattaaattt gatataacaa aatttgattt
    15121 tgacatacca aattttgata tacatgaaat cataaaaaaa tttaataagt agtaataagt
    15181 gaataaggtt tattgattaa aataagcctt attttagtta tatattttta atgtataaaa
    15241 taaaaattca ttaactgtat tttataggat ttactttaag tttatgatat tatataatat
    15301 ttagtgttag acttgataat ccacatataa tgtggataat tttgtttagt gccaattagt
    15361 ttatattcaa aaattttaaa tttggtatat aaagagtttt tagataatgt aaaataataa
    15421 ctataagaat aatcaagagg agggaatttt aaggtggaca ataaacaaag tagaactaga
    15481 aattttagta taattgcaca tatagaccat ggaaagtcta ccttagctga tagattaata
    15541 caacaaactg ggcttgttag tgaaagagac atgaaaagtc aattacttga caatatggac
    15601 cttgaaagag aaagaggaat cactataaag cttcaaaaca taagattaat gtataaagcg
    15661 aaggatggaa atgaatatta tttaaatctt atagatacac ctggacatgt agactttaac
    15721 tatgaggtat caagaagttt agctgcatgt gaaggagcac tattagtagt tgatgcagct
    15781 caaggtgttg aggcacaaac tttagccaat gtttatcttg ctatagacca agatttagaa
    15841 atattaccaa taataaataa aatagattta ccaagtgcaa gaccagagga agtaaaaaat
    15901 gaaatagaag atttaatagg gcttgattca agtgaagctc ctcttatttc agctaaaact
    15961 ggtttaaaca tagaagatgt tttagaagat attgtaaaaa atgttcctcc acctaaagga
    16021 gataatgaag ctccattaaa ggcattaata tttgattctt actatgatgc ttacaaaggt
    16081 gttgtagctt atgttagagt atttgaagga actgttaaaa agggtatgac aataaaaatg
    16141 atgaatacca ataaaaagtt tgaggtaact gaagttggtg ttatggctcc aggtcaaact
    16201 gaacttagtg aattatcagc aggtgatgtt ggttatattg ctgctagtat aaaagatata
    16261 agaagttgtc gtgttggtga tacaataaca gattcaaata atcctacaga agaaccttta
    16321 ccaggataca aaaaagctac tccaatggta tattgtggta tatatccagg agaaggagaa
    16381 aagtatgaga atgttagaga tgctcttgaa aagcttcaag taaatgatgc tgcacttgaa
    16441 tatgaagctg agacttcagc agcactagga tttggtttta gatgtggttt cttaggtctt
    16501 ttacatatgg agataatgca agaaagactt gagagagaat ttaaccttga tataattact
    16561 acagctccat ctgttatata tagagttaca aaaatggatg gagaggttgt aatgatacaa
    16621 aatcctgcaa acttaccaga accatctgag ataaagatga tagaagagcc tatagtaaaa
    16681 ggagatataa tagttcctaa agactatgtg ggtgttgtta tggagctttg ccaggaaaga
    16741 cgtggaaata tgttaaatat ggaatatata gatgatagaa gagtaatgct tcactatgac
    16801 cttcctctca atgaagttgt ttacgatttc tttgatgctt taaaatcaag aactagaggt
    16861 tatggttctc ttgattatga ggttaaaggt tatgtagcat ctacacttgt aaaacttgat
    16921 atattaatca ataaagagca agtggatgca cttagtttta ttgtccatga gacacgtgct
    16981 ttccctagag gaaaggcaat gtgtgagaaa ttaaaaggag aaattccaag acatcagttt
    17041 gctataccaa tacaagctgc tgttggtaat aaggttattg caagagaaac aataagcgct
    17101 ctaagaaaag atgttcttgc taaatgttat ggtggagata tatctcgtaa gaagaaactt
    17161 cttgaaaaac aaaaagaagg taagaagcgt atgagacaaa ttggttctgt tgaagttcct
    17221 caaaaagcat ttatgtctgt gcttaaatta gatgagtaaa tccttataaa tttagataaa
    17281 gatatttaaa agctagagtg attttagaat tatttctaaa ttagtctagc ttttttatgg
    17341 tatattttaa gtaaactcat agagaatata agtaagaata ttaaagaaga ataaaaaggt
    17401 gatatttatg attaatatag aaattcctaa aaaagtagac tatataatta aagaattaga
    17461 aaagaatggt tatgaagctt atatagttgg aggatgtgtt agagattgtt tattagaaag
    17521 agttccaaac gattgggata taacaactag tgctagacca gaagtggtag tagaattatt
    17581 tgaaaaaaca attccaactg gtatacaaca tgggactgta acagttatga ttgaacatga
    17641 accatttgaa gtgacgactt atagaattga tggaaattat agtgatggta gacatccaga
    17701 ttcaatagaa tttacaaata acatagttaa ggacttgtca agaagagatt ttactatcaa
    17761 ttcaattgca tacaattcaa agacaggatt agtcgaccca tttaatggct atgaagatat
    17821 acaaaataag tatatacgtt gtgttggaaa tccagtagat agatttgaag aagatgcact
    17881 tagaatgctt agagcagtta gattttcagc acaactcaat tttaaaatag ctgaaggtac
    17941 taagcaaagt attcataaaa aagcagattt gataaaaaat gtttctattg aaagaatcca
    18001 gacagagttt aataaaatat tagtatctga ttcatctaaa ttaaacttat taaaatcaac
    18061 aggtttgttg aaatttataa ttcctgaaat atgtgaatta gaagatgtta ctcaacacaa
    18121 tccatatcac atctatgatg tacaaaaaca tactttaata gctacagaag caattgaaga
    18181 tgaactgtat ttaaaactta caatgttgtt tcatgattta ggtaaaaaag taactaaaac
    18241 aactgataaa aatggtgttg accattttta tactcatagt agagaatctg taaagattgc
    18301 aaagaagatt ttgaaaagac ttaagtatga taattatact attaataaag ttttaatatt
    18361 aatacaatat catgattata gaatagaacc taaaaggaag ataattaaaa agttattaaa
    18421 taaacttgaa gatgttgaat tatttgaaga tttaataaaa gttaattggg ctgatacact
    18481 tgccaaaaat ccaaagtatg caaaacaaaa gatattaaat cttattgagt gtgaaaaaga
    18541 atttaaacat ataataaatc aaaaggagtg ctttaatctt aaagatttgg ctattaatgg
    18601 aaaagatttg atatctatag gtataaaacc aggtaaagat ataggacata ttttaaataa
    18661 gatgcttgaa atagtcataa ataatccaga gttaaatgaa aaagaaatac taaaagaaaa
    18721 agcattaaa
//
LOCUS       DAGSRM010000078        18660 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.078, whole genome shotgun sequence.
ACCESSION   DAGSRM010000078 DAGSRM010000000
VERSION     DAGSRM010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18660
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.078"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            <1..321
                     /locus_tag="MJE33_002765"
     CDS             <1..321
                     /locus_tag="MJE33_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBY9648692.1"
                     /translation="VEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDS
                     EITQYSFCTNGSHYAGEAGIRTIGFGPSKENLAHTIDEYIELDQLFKGTEGYYGILKS
                     VYKK"
     gene            337..1920
                     /locus_tag="MJE33_002766"
     CDS             337..1920
                     /locus_tag="MJE33_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HBY9648693.1"
                     /translation="MRTLIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDYNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKANWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDTIGKSLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIGFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAARSFSIKDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            1948..3276
                     /gene="ssnA"
                     /locus_tag="MJE33_002767"
     CDS             1948..3276
                     /gene="ssnA"
                     /locus_tag="MJE33_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HBY9648694.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTEEMKVKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEMAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISAEEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DPRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIINGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            3421..4773
                     /locus_tag="MJE33_002768"
     CDS             3421..4773
                     /locus_tag="MJE33_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBY9648695.1"
                     /translation="MSRDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     SAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
     gene            4965..6074
                     /locus_tag="MJE33_002769"
     CDS             4965..6074
                     /locus_tag="MJE33_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBY9648696.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMLMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIKGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            6357..7748
                     /gene="hydA"
                     /locus_tag="MJE33_002770"
     CDS             6357..7748
                     /gene="hydA"
                     /locus_tag="MJE33_002770"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBY9648697.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGLDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQAKGCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKSENNLSPKYHQLTRPAIMEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKQRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAILDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNPAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEAKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            7742..10303
                     /gene="xdh"
                     /locus_tag="MJE33_002771"
     CDS             7742..10303
                     /gene="xdh"
                     /locus_tag="MJE33_002771"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HBY9648698.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRSKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTADALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEDDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETANLLPENIVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVILDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSVPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
     gene            10455..11801
                     /gene="rpoN"
                     /locus_tag="MJE33_002772"
     CDS             10455..11801
                     /gene="rpoN"
                     /locus_tag="MJE33_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBY9648699.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
     gene            12599..13621
                     /locus_tag="MJE33_002773"
     CDS             12599..13621
                     /locus_tag="MJE33_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBY9648700.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
     gene            13681..14688
                     /gene="gap"
                     /locus_tag="MJE33_002774"
     CDS             13681..14688
                     /gene="gap"
                     /locus_tag="MJE33_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBY9648701.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            14910..16112
                     /locus_tag="MJE33_002775"
     CDS             14910..16112
                     /locus_tag="MJE33_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBY9648702.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKAKNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            16138..16881
                     /locus_tag="MJE33_002776"
     CDS             16138..16881
                     /locus_tag="MJE33_002776"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBY9648703.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEVKDKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKVQVEKALENVLKDDLAK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            16890..18422
                     /locus_tag="MJE33_002777"
     CDS             16890..18422
                     /locus_tag="MJE33_002777"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003228330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HBY9648704.1"
                     /translation="MMKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTL
                     INASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFTNKVLCEAMD
                     NAKENSLHVMGLLSDGGVHSHIDHLKAIIKMAKDKGVQKVYVHAFTDGRDTDPQSALE
                     YAKEVQASMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAI
                     QNSYDDGKNDEFIMPTVIMKDDKPAGSIKENDSIIFFNFRPDRARQITRALVCEEFDG
                     FKREDIKNFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAH
                     VTFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALDKLGEDKFDFIVL
                     NFANPDMVGHTGSIEAAIKAVETVDTCVGKLIDKIVELGGSAIITADHGNAEYMLDPE
                     TGKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTVLDMMKLEKPEEMTGHSLI
                     SK"
BASE COUNT         7490 a         2150 c         3746 g         5274 t
ORIGIN      
        1 gttgaaaaag ctgattgcta tacaggaaat actatagaaa gtgaaagatt cttcccaggt
       61 tggttatatg atgaagaaga tgaatttgtt caagcagcgt ataagggatt aaaagaagct
      121 ggaatagatt cagaaataac tcaatactca ttctgtacga atggaagtca ctacgcaggt
      181 gaggctggaa taagaactat aggatttggt ccttctaagg aaaatcttgc tcatacaata
      241 gatgagtata tagaattaga ccaattattc aaaggtacag aaggatatta tggtatatta
      301 aaatcagttt ataaaaaata attaggagat ggttttgtga gaactttaat aaaacatgga
      361 cttatagtag atggaaataa aacaccagca tatgaaggag atattttaat agaaaacgag
      421 aaaatattaa aaatatcaca agatttaaac gaagaagctg acaaagttat agatgcaaaa
      481 ggtagagtta tatgtccagg tttcatagat actcatagcc attcagacct agttatttta
      541 gttaatccat ataatgaggt aaagattaga cagggaatta caacagaagt gttgggccaa
      601 gatggtatat ctatggcccc tctacctcaa gagcatatat cttcttggag aaaaaattta
      661 gcaggtttag atggtgaaag tgatgaaatt gattggaaat atgaaacaac agagaattat
      721 ttaaaaatga tggattacaa tggggtaggt ttaaatgaaa cttatttagt acctcatgga
      781 aacgttagaa tggaagctat gggacttgaa gatagacctg ctactaaaga agaaattcaa
      841 aaaatgtgtg aaatcacaga gagagaatta aaagcaggag ctataggatt gtctactgga
      901 cttatatata taccttgtgc atactcattg actgaagaaa ttatagagat gtgtaaggta
      961 gtagcaaaat atgacggagt atttgtagtt caccaaagaa gtgaagcaga tacaatttta
     1021 acttcaatgg aagaaatcat agaaattgga aaacaatcag gagtaaaagt tcatttttca
     1081 cattttaagg tttgtggtaa agcaaactgg aagtatatac cacaagttat agagctttta
     1141 gaaaaagcag aaaaggaagg aataagagta tcttttgacc aatatccata tgcagcagga
     1201 agtacaatgc ttggagtagt tttacctcct tgggcacatt ctggaggaac tgataagtta
     1261 atagaaagat taagtgatga aaacgaaaga gcaaaaatga aaaaagatat agcaaatgga
     1321 atagaaggct gggataattt tatagaattt gctggaatag accaaatatt tgtaacaagc
     1381 gtaaaaacag aaaagaataa agatacaatt ggtaagagct tattagaaat tggaaaaatg
     1441 agaggaaaag acccattaga tgcaacgttt gatttattaa aagaagaaga aaatgcagtt
     1501 gggatggtag atttctatgg attagaagaa catataatag gattcatgaa aagagacgag
     1561 caaaatgtgt gtacagatgg tcttttagca ggtaaaccac atccaagagc ttatggttct
     1621 ttccctaaaa tactaggaag atatgttaga gagttgaatg tgttaacaat cgaagaagct
     1681 gtttataaaa tgactaaaaa ggcagctaga agctttagta taaaagatag aggcgaatta
     1741 aaagaaggat attttgcaga tatagttata tttgacaagg atacagttag tggttgtgat
     1801 gattttatca actctatgca gtaccctaca ggtatagatt acgtaattat aaatggaaat
     1861 tgtgttgttg aggaaggaaa atacaataaa attaaagcag gaaaagtact taaaaattaa
     1921 atcttagctt taaggaggaa taaatttatg attttagtgg ggaatgggag gctaataagc
     1981 caagattctt taaatcccta tatggaagat ggatgtgtag taataaatga taatctaatt
     2041 gaagatatag gtactacaga agaaatgaaa gttaaatatc cagtacatga atttatagat
     2101 gcaggcaaga aaataatcat gccaggtcta ataaattcac atatgcatat atacagctca
     2161 tttgctagag gaatggcagt tccaggaaaa ccttctgaga actttttaga gatactaaac
     2221 aatttatggt ggagacttga caaacagctt acactggaag atacaaaata cagtgcatat
     2281 gcaacgtata tagaatgtat aaaaaatggt gtaacaacag tttatgacca tcatgcaagt
     2341 ccaaatgcaa ttgaaggcag tttgtttaca atatcagatg ttgcaaagga tttgggaatt
     2401 agaacgtgtc tttgttatga agtgtctgat agagatggtg gaaatacaat ccaacaaggt
     2461 atcaatgaaa acgtaaactt tataaaatac gctcaaaaag atgatacaga catgataaaa
     2521 ggaatgtttg ggcttcatgc tgcatttaca ctatctgatg atacattaaa taaatgtgct
     2581 tcagagatgg ctggtcttga tacaggttac catgtacatg ttgctgaagg actagcagac
     2641 ttacaatata atgtagataa atatggaaaa agggttttag aaagattcaa tgattttgga
     2701 gtactaaaag aaaaaacaat agcagttcat tgtatatata taagtgcaga agaaatcagt
     2761 cttttaaata aaacaaatac atttgtagtt cataatccag aatctaatat gggaaatgca
     2821 gttggatgtt ctcctgcaat agagttatta aaaaggggag ttacagtagg attaggaact
     2881 gatggatata ctagcgatat gtttgaatca ttaaaagttg ccaatataat tcacaaacat
     2941 cacttaaaag acccaagagt tggatttgaa gaatcacaaa caatgttatt tgataacaat
     3001 agaaaaatag ctaaagctta ttataaaaat gacctaggag tacttaaaaa aggtgcttat
     3061 gcagatgtta tagtagttga ttatatacca catactccat tgacagaaaa tacaataggg
     3121 ggacacacta tttttggaat gagtggaaga agcgtagata caactattat aaatggaaaa
     3181 gtacttatga aagatagaaa gttaataaac atagatgaag aaagtattct agcaaaaagt
     3241 agagaattaa gtcaaaatct atggaataga atttaaataa ttgatattaa tatacaagta
     3301 agaaacaaga atttagatgt taaaaacaga gcttaaacaa aaataggaat ttaaatgaag
     3361 tatataaata aaaaatatac ggaatagaat attaaattaa atatagaagg gagacccaaa
     3421 atgtctcgag ataaaagccc taaaatagct ccagtagatg aaaggttacc tgtatccaaa
     3481 tcttgggtat tttcactaca gcacgtttta gcaatgtgtg caggagcagt agcagttcct
     3541 atgatgatag gaagtgctgc acgactagat catgctagta ttgttttttt aataaatgct
     3601 tgcttgttta tggcaggtat aggaacactc atacaaagtg ttggtatagg aaatattgta
     3661 ggtgctaaaa taccagtaat agaaggaact agttttgccg ctgtggcagg tatgacagca
     3721 atagcctcat catatagtga ttcacaagtt gcaatacaga cagtatttgg ctctgtaata
     3781 gtagctggat tgttttgtat attacttgct ccagtatttg aaaaattaat aagatttttc
     3841 cctagtgtag ttactggaac agttgttatg gtaataggta tttcactatt tccagttggg
     3901 ataaaatgga taactgacaa caaattagaa gcggttgaac caaactcatt agcattagct
     3961 ttagcagtat tggcaataac tttactttca tttaaattct taaaaggaat ttggaatagc
     4021 acagctatat tggtaggtat agtacttggt acactacttt ccatggcagt tggaatggca
     4081 gattttagtg cagtaaatgg agttggttgg cttaacttaa atgtaccttt aaaatttgga
     4141 atgcctagat ttgaaccatc tgcaatacta tcattgtgtt tgattatgtt ggtacttatg
     4201 acggaatcag ttggtaatat gatagctatt catgaaatgg tagacaaaga agtaacaggt
     4261 aaaaatatta aaagaggtct atttggagat ggaatatcta cagcattatc tggaatattc
     4321 aattcatttc caataactcc atttggtcaa aatacaggaa tagtaggtct tacaggtata
     4381 aaatcaagat ttatagcagt ttacgcaggg gttatattgc tagtacttgg attcttacca
     4441 aaatttgcag caataatagg agcaatacct aagcctgttc ttggaggcgt aggattcgca
     4501 atgtttggaa tggtaatggt tggaggagta aaaactttaa gcaaagtaaa atttgatgga
     4561 actaaaaatg gggttatagt agcagttagt gttggtttgt ctatgatacc actaacaaat
     4621 cctgatttct atcataattt cccaacttgg gtacaaacaa tattccatag tggaataaca
     4681 acaggtagtt tatcagcagt gcttttaaac atattcttca atgaactagg ttctaaaaaa
     4741 gaaaaaaata ataaagttga agaagcaaaa taaaaataaa acaagtaaaa atctatattg
     4801 acttaaagtc attaagtatt aataaaaata attcagagat attaataatt caacaaaact
     4861 atcttatatt ttaaataata taaaaaagta aataaataac gaatacaaat aaaagaatac
     4921 aatctaaata ttaaaaatat ttaatttaag ggagagataa taatatggct aatttaaaag
     4981 ttaatttata tggaatggaa tttttaaacc caattatgac agcggcagga cctggagcaa
     5041 aagatggtgc tctctgtcaa gaggcagcaa aaggtggagc aggagctata tgtacaaaaa
     5101 ctatatctgt attaccagct gatgtaccaa gaccatgtat ggcaaaaact aatagtggtt
     5161 ttttaaacac agaattatgg tcagagcttc caaaagagca atggatagag gaagaataca
     5221 aaaaggtaaa agaaacaggg cttccagtaa tagtaagtat gggatattca gcagagcaaa
     5281 tagctgaggt tgctccttta gtaaagcctt atgcagatgc agttgaatta tctactcatt
     5341 atgtaggtac agacgttgca ccaatagtaa atgctttaaa agctgcaaaa gctgttttag
     5401 atgtaccagt atttatgaaa atgagtcctc atactgacat tcaaactatt gcaaaagctg
     5461 ttgaagaagc aggtgcagat ggacttgtaa tgataaactc atttggacca tgtatggcaa
     5521 tagatgtaaa tacaggatac cctataatgg gaagtaaagc tggatacgga tggttatcag
     5581 gtgcaccaat aagacctcta gcaataagat gtatatatga agcttctcaa gttgtaaata
     5641 taccaataat cggtgttggt ggaatcactt gtggaaaaga cgtggctgaa atgttgatgg
     5701 ctggagcaag tgctgttcaa gtatgtacag aagcaatcct taaagggcct tctatctatg
     5761 gaaaaattgc taaagaatta aatgaatttt tagatgagaa tggatatgct gatgttaatg
     5821 aaataaaagg attaactcat aagaaaatgg cagctagagg gtttagaact cattcagtag
     5881 ctccagtagt ggatgataac aagtgtatca aatgtaagca atgtgtaaca agctgtgtat
     5941 acgaagcttt agaagttaca gataagttga acatagatgc agacaagtgt tttggatgtg
     6001 gattatgtgt aactagatgt ccaaaaggag ctttaagcat ctgctacaat caagtagaag
     6061 ctatgaatga gtaataatta tatagaattt tggtaattaa cttagagtaa taggtattag
     6121 gataataaaa tatatcagat aaacaaacac atattatgca ttaataatac agtctaaagt
     6181 taataatttt attatattga tttaactaaa tattagttta gctgaatatt aatttaatta
     6241 ggtattggtt taatcttaat atagatttac tacgtataag tgaactaact agattacttt
     6301 aagttaattt tttcatataa aataaattga tacattacaa gtaaggtgga gtctatatgg
     6361 gaattgtttt aaaaggtggg aaagtagtaa caagtgaaaa aacttactgt gcagatgtaa
     6421 gaatagaaga tgaaaaaata gtcgatatag gtcttgatat atcaaaagaa aatgatgaaa
     6481 tagttgatgt tagtgattct tatgttatac caggaggtat agatacacac acacactttg
     6541 acttagatac aggcgttact aagacagcag ataactttga gacaggaaca aaagctgcta
     6601 tagtaggagg aactactact atattggatt ttgctacaca agctaaagga tgttctttga
     6661 aagacggatt agaagaatgg catgaaaaag ctaaggataa atcctattca gattatggct
     6721 ttcatatggc tataacagat tggaatgact cggtttgtaa tgaaatggag gatatggtga
     6781 aagagggagt atcttcattt aaactgtata tggcgtataa aggaagcctt caagttgatg
     6841 atggtgtaat ctttgaagca ttgagaaaag cagaagagat aggaggaatt attggtttcc
     6901 attgtgaaaa tggggacata atatgtgaac ttgtggataa agcaaagtca gaaaataatt
     6961 tatctccaaa atatcatcaa ttaactagac cagctataat ggaggcagaa gctacatcaa
     7021 gacttatgaa gatagctgaa gttgcaaatt ctaaagtata tgtagttcac ttaagctgta
     7081 aagaatcctt agaggaggtt ttaaaagcta agcaaagagg tgtggatgta atagttgaaa
     7141 cttgtcctca ataccttcta ttaaatgatg aattatatga tttagatgga tttgaaagtg
     7201 ctaagtatgt aatgtcacca cctttaagaa ataaagaaaa taatgaaata ttatggaatg
     7261 ctattttaga taatcaaata gacactatag gaacagacca ttgttctttt aattataagg
     7321 gacagaagga acttgggata aacgatttca gcaagatacc aaatggttct ccaggtgtag
     7381 aacataggat gagtctttta tacacatatg gagtctgtaa aaataaaata tctatcaaca
     7441 agatggtaga agtaacatca actaatccag caaagttgtt tggaatgtat cctaataaag
     7501 gaactataga aataggcagt gatgcagata tattagtctt aaatccaaac aaaaaatcca
     7561 aaatagaagc aaaaaaacaa ttacaaaatg tagattatac accttatgaa gggtatgaag
     7621 tggattgtca aatagaacat gtttatttaa gaggaaaaga agtagttaga gatggagaat
     7681 taatttgtaa tactccaagt gggagatata taaataggcg tctaacaaag ggggctagag
     7741 aatggtagaa tttatattaa atggagagaa agttgttgca aaagaaggca aaaaactttt
     7801 agacttcctt agagaagata tgaatataac ttcagttaaa aatggatgtt cagaaggtgc
     7861 ttgtggaaca tgtatggtta taatagatgg taaagctact aaagcatgtg tatttaaaac
     7921 tgataaatta gctgggaaaa acataacaac tatagaggga ttatcagaga gagaaagaga
     7981 tgtttttgca tattcttttg ctagtcaagg agctgtacaa tgtggatttt gtattccagg
     8041 tatggtaatt agttcaaaag cacttataga cgttaataat aatcctactg aagatgatat
     8101 aaagaaagct ttaaaaggca atatatgtag atgtacaggg tatgtaaaaa taattaaagc
     8161 tgtcgattta gcggcaaaga tttttagaga aaatatagat gtgccaaaag taaactgtaa
     8221 gggtttagta ggtgaaaatc ttcctagagt tgatgcagtt ggcaaagcac ttggatattc
     8281 tgaatatgta gatgatatga gaattgaggg tatgatttat ggttcagcag ttagatctaa
     8341 gtatcctaga gctttagtta aaaaaataga tatagaagaa gcaaaagctt atccaggagt
     8401 tgttgcagtt ctaacagcta aagatattcc aggaactata aaggtaggac acttaaagaa
     8461 ggattgggat actttaatac cagaaggtga aataactaga tacttaggag atggaatagt
     8521 tttagtagca gcagaaacta gagaagcgtt agaagaggcc aaaaagctag ttaagataga
     8581 ttatgaggaa ttaacaccat taagtactac agcagatgca cttagagaag atgctccaaa
     8641 gatacatgag acagggaata tacttgttaa ggaacattta gtaagaggaa acgctaaaga
     8701 aaagatagaa aattcaaaat atgttgtaac taaaagatat actacacctg ctacagaaca
     8761 cgcattttta gagcctgaat gtgcagtagc tggtccttat gaagatgatg gagtaataat
     8821 ctattcaaca gaccaagggg tgtttgccac tcaacatgaa tgtgcagata tgttaggatt
     8881 accatatgag aaggtaagag ttataaacaa aatggtagga ggagggtttg gtggaaaaga
     8941 agacatgagt gttcaacacc atgcagcctt acttgcatac catacaaaga gatttgtaaa
     9001 agtacagtta acaagaaaag aaagtattat tgttcatcca aaacgacatg ccacagaaat
     9061 ggaatttacg acagcatgtg atgaaaatgg atacttaacg ggtatgaagg caacgataat
     9121 atctgacaca ggtgcatatg cctctcttgg aggaccagtg ctacaaagac tgtgtactca
     9181 tgcagcagga ccatacaatt atcaagatat tgatataaca ggaacagcag tatatactaa
     9241 taaccctcct ggaggagcat ttagaggatt tggagttaca cagagttgtt ttgcaactga
     9301 atgtaatcta aatttacttg ctgaaatggt tggaatatct ccttgggaga taagatataa
     9361 aaatgcaata agacctgggc aagttttacc aaatggtcaa tttgcagatg aaggaacagc
     9421 aatggcagag acactagaag ctgtaaaaga agagtatgaa aagcatccac gtgcaggtat
     9481 agcttgtgct atgaaaaata gtggggttgg agttggtata cctgatgttg gaagatgtag
     9541 attggtgata aaagatggca aagttcacat aagaacaagt gcttcttgta taggtcaagg
     9601 gcttggtact gttatgactc aagtagtttg tgaaactgct aacctacttc ctgaaaatat
     9661 agttcatgaa gttccagata ctcatttaac accagactct ggtaatacta cggcttcaag
     9721 acagacagta tttacgggag aagctactaa ggttgcatca aataagttaa aagaagcttt
     9781 agactctggc aaaactttac ttgatttaga gggagaagag ttttatggag aatatgaaag
     9841 tattacagat aaaatgggtt gtgacaaaga aaatccagta agtcatgttg cttatggata
     9901 tgcaacacaa gttgtaattc tggatgaaaa tggaaaacta gaaaaagtag tagcagcaca
     9961 tgatgttgga agagctataa atccaatcag tttagaaggt caaatagaag gtggagtagt
    10021 tatgggtctt ggatatgctc taaccgaaga ttacccagta gtcaactctg ttcctacagc
    10081 taagtttggt acattaggtt tatttaaatc tacagatata ccagaaatac aagctttaat
    10141 agttgaaaag aacaaagatt tattggctta tggggcaaaa ggtgtaggag aaatttgtac
    10201 agttcctact gcaccagcag tacaaaatgc ctattatgta tttgataaag aatttagaac
    10261 tgagttacca ctacaaaata ctccatatag taaaaaaaag taacaaaaaa aagaaatatt
    10321 aaataaaaaa atagatgaaa ttgtaaataa aaaaaaccct tttgcataag gtatatttta
    10381 aatgatagaa tatacatata caggagggtt tttcttttat aaaataagtt taataaaatt
    10441 gagaggtgag cagaatgaat tttaataata gtctagaatt aactcagtct cagaagctaa
    10501 taatgactac acaacttaag caatcattat caatattaaa tatgagtaag ttagagttag
    10561 aagaagagat aaaaagagaa gcagagaata acccattagt tgaagtcgag aaaagtgggg
    10621 agataaattg ggaagagtat ataaaagaca tggataggtc aagacgttta gatagaacag
    10681 agataagcta taatcctgat aatgaagtta atctagaaaa tttagttaaa tattcgtcaa
    10741 atttatatga agatttaaaa tttcagataa gcctgtataa acttacagac aaagaattgg
    10801 aagtctgtga gtatataatt gatagtttag atgaggatgg atatttaaga actgaggaaa
    10861 aagtaattgt tgatacctta aagatagatg aagaactttt tgaaaaatgt ttaattagtg
    10921 ttcagcagtt ggaacctagt ggagtagggg caagaagtct ttcagaatgt ctgatgatac
    10981 aaatggctag tttaggaata tataatgaaa ttttagagga aatcgttaca aaagacttaa
    11041 atttaattgc aaataataaa tataaagaga taagtaaaaa gtacaatatg tcattacaaa
    11101 aatgtgttga actaataaac ataattaaga cattagaccc caaaccagga agaacttgtt
    11161 cagtagagaa aagtgtatac attcaacctg atgtaacagt agaaaaaatt gatgatgaat
    11221 ttattgtata tttaaatgag aaagattctt atcaaataag aatcaatagt tattataaag
    11281 acatattaaa aaattcacag tctgatgaaa gtgcaaaaga atttataaaa gaaagactaa
    11341 attcagcaac aggacttatg aaaaatatag agagtaggaa aactacagta cttaagattg
    11401 cagaagagat agtaaaagct caagatgaat ttttgagaaa agggacaaaa tatataaaac
    11461 ctttgaaaat gaaagacata gcagaaaaat tagaatttca tgaatctact ataagtagag
    11521 gtgtaaatgg taaatatatg ctaacacctt ttggcgtata tgaatttagg tactttttca
    11581 gtagcgccat tgagactgag aataatgaaa tggcatctag tacaagcata aaaaaaatta
    11641 taaaggaaac aataaaagac gaaaataaaa agaaaccttt aagtgatgac catatatcta
    11701 agttactaaa agaaaaagga atcaatgttg caagaaggac agttgccaaa tatcgtgaag
    11761 aattaggaat cccatcttca agtaagagaa aagaatacta ataagtaaaa gacaaatatc
    11821 agtatggtaa ggggaaaagg ctaattaaga gatgaatgta gattgggcta aaaattttat
    11881 aaagtaaaat aatataatat gaaaaagtat taaaatgcta taaggtatta aaaaactggg
    11941 gaacattaaa acattaagat agcaaagaac ataaaaaact aaagaagatt aaaacattaa
    12001 agaacattag agctttgagg aatattagaa ctttaaagaa tattaaaatt tataaaatat
    12061 aaaaatacta aaaagaattg aaaaactggg gaacattaaa atattgtaaa atgctaaaaa
    12121 actagaaaac ataattaaat aagtgcttat aaagaatgat taaattaaat aagtgtttgt
    12181 aaagaataat tagatattag aaaaagaaga atacctagat aaataaacta ttattaaatt
    12241 taataatcac aaaaggaaaa cacgtagggt ataaataaaa atcttaacta gataaaagta
    12301 aacttagttg tagataaaaa taactagaaa taaaagtaac tagaaataaa attatttata
    12361 gtaataaata ttagaatgaa aatataaaaa aatatatata aaaagcctta tataaaaaga
    12421 aaaaatactg tgatttgaaa atggtggaga aatattttat aaaataaaaa aataaaaaag
    12481 tttttgaaaa actattgaaa tctatacgca aatcctataa aataaaaata tcttgtggga
    12541 cttaaataaa cactagggac aaaaaaagtc ccgaaaggaa agcaataagg agtatgttat
    12601 gaagaactta ttagaaattc aaaagaaatt gattccacag gcaattgaac ttatggaaag
    12661 aagatactct attttaagac aaatatcttt aagtgaacca ataggtagga gaacattatc
    12721 aaatgtatta gagattagtg aaagaataat caggtcagaa actgagtttt taaaagaaca
    12781 aggacttata gacgtagctg tatctgggat gactatcact aaagaaggtt ctgatttgtt
    12841 agataaactt aaggacatca tgagtgacat catgggattg tctaagttac aagatagagt
    12901 cagagaaaag cttggaataa aaagggtaat attagttcct ggaagctgtg atgaaaatgg
    12961 aagtttacta aaagatgtaa ctagatatgg atgcgaatat ttcttaggca ttttaaaaga
    13021 tggagatatc gtttcaataa caggaggaag cactatgttg gagttttcaa acgttattaa
    13081 aactgataag agatatccaa actctacaat agttccagct agaggaagta tgggaactga
    13141 tgtagagatg caatctaaca atatagttgc ttcaataagt aagaaattac attctaattg
    13201 caaactttta catataccag atgagttgga tgaaagtgca atgaaaactc ttagccaagt
    13261 gccagagatt aagacaactt tggagtatat taaaagaaca gacattttag tttttagtat
    13321 aggtagagca gatgtcatgg caaagagaag aaaacttcca gaagacaagg ttgatgagat
    13381 attatctaaa aatgcagttg gagaagcatt tggatattat ttcaacaaag atggagaaat
    13441 agtttacaaa ctgaacactg ttggaattga tttagaaaca gttaaacaag taaaagaaac
    13501 tattgcaatc tttgcaggga ctgaaaaagt agaagcctta ttagctatat caaatatgca
    13561 taaaaacatg gttcttgtaa cagatgaaga aagtgcttat aagatattgt cattattata
    13621 aaagtcaaga cctagcacag agctagaaaa catacattta ctttatagga ggtagcgaaa
    13681 atggttaaag tagctataaa tggatttgga agaataggaa gattagcatt aagaaaaatg
    13741 atggagcaac aagataagtt cgaagttgtt gcaataaacg acttaacaga cgctaaaatg
    13801 ttagctcatt tatttaaata tgatacagct caaggaagat ttaacggaga aatagaagtt
    13861 aaagaaggtg cttttgtagt aaacggaaaa gaaataaaag taacagcaga aagaaatcct
    13921 gctgacctac catgggctga attaggagta gatatagtat tagaatgtac aggattcttt
    13981 acatctaaag ataaagctga agctcatata caagcagggg ctaagaaagt tgttatatct
    14041 gcaccagcta ctggagattt aaaaacaata gtattcaaca caaacagtga catattagat
    14101 ggttctgaaa cagttatatc aggagcttct tgtactacta actgtttagc tccaatggct
    14161 aaagtattaa atgataaata cggtatagaa aaaggattaa tgactactat acatgcatac
    14221 actaatgacc aaaatacttt agatggtcct catccaaaag gtgatttaag aagagctaga
    14281 gctgctgctg gaaacatagt tccaaacact acaggagctg caaaagcaat aggcttagta
    14341 ataccttcat taaaaggaaa attagatggg gcagcacaaa gagtaccagt tgtaactggt
    14401 tcaataactg aattagtttg tacattagga aaaaatgtta cagttgaaga aataaatgca
    14461 gctatgaaag aagcttcaaa cgaatctttc ggatatactg aagaaatgtt agtatcttct
    14521 gacataatcg gaataagcta cggttcatta tttgacgcta ctcaaacaaa agttatggaa
    14581 gtagatggaa aacaattagt taaagttgtt tcttggtatg ataatgaaat gtcttatact
    14641 tctcaattaa taagaacttt aggatacttt gcacaattag caaaatagtc agttttgaga
    14701 aaatagaaaa gtagattgac aaagtccgag tttgtagttt aatactacgg actcaggact
    14761 ttttttaaga aaaaatatat aaaatacacc taaataatgt tacatactat atataaaaat
    14821 atgataatat gatatattta atagggtaaa agtatgtact atattatact tggcaataaa
    14881 aaattacttt attaaaagga gagatattta tgtcaatgct taacaaaaaa actatagaag
    14941 atattgatgt gtgcggaaaa aaagtattag taagatgtga ctttaatgtg ccattacaag
    15001 atggtgtaat tactgatgaa aatagattga acggagcttt accaactatt caatatttaa
    15061 tttctaaagg ggcaaaagtt atattatgct ctcacttagg aaagccaaaa ggagaagcta
    15121 aaccagaatt atctttagca ccagtagcaa agagattatc tgagatgtta ggaaaagaag
    15181 ttgtatttgc agcagacgat aatgttgtag gagaaaatgc aaagaaagca actgaaaaaa
    15241 tggaaaacgg agatgtagta ttattagaaa atactagata tagaaaagaa gaaactaaaa
    15301 acgaagagaa tttctctaaa gaactagcat cacttgctga aatatttgta aatgatgcat
    15361 ttggaactgc tcatagagca cattgctcaa cagttggtgc aggcgagttt ttacaagaga
    15421 gagtttgtgg atacttaatt caaaaagaat taaaattctt aggagaagct gttgcaaacc
    15481 ctgtaagacc atttacagct atacttggag gagctaaagt ttctgataaa ttagctgtta
    15541 taaacgaatt attagaaaaa gttgataatc taataatagg tggaggaatg gcttatacat
    15601 tcttaaaagc tcagggttat gaagtaggaa cttctttact tgaaatagat aaagttgaat
    15661 atgctaaaga aatgatggaa aaagctaaga ataagggtgt taacttatta ttaccagtag
    15721 acgttgttat ggcagaccac tttgcaccag atgctactcc aatagtaaca gaagatgcta
    15781 atgtaaaaga agattacatg ggactagata tgggacctaa gactatagca aactttgtaa
    15841 aaactataaa agaatcaaaa actgtagtat ggaatgggcc aatgggagta tttgaatttg
    15901 aaaactttgc aaatggtaca ttatcagtag ctagagctat ggcagagttg actgatgcaa
    15961 ctactgtaat tggcggtgga gatagtgcag cagcagttaa tcaattagga tttggtgaca
    16021 agatgactca cgtatcaaca ggtggaggag catcactaga atttttagaa ggtaaagaat
    16081 taccaggtat tgcagcatta gataataaat aaactaatat atagaggaga ttgtaaaatg
    16141 agaaaaccta taattgcagg aaactggaaa atgcataaaa caataaaaga agctttagaa
    16201 tttgtaaatg aagttaaaga taaagtaaat tctgataaag tagaagctgt tatatgtgca
    16261 ccatttactt tattaaagga tttaaaagaa gctactaagg gtacaaatat aaagataggt
    16321 gctcaaaata tgcactttga agaaaaagga gcatttacag gagaagtttc acctcttatg
    16381 ttaaaagaaa tagatatgga ctatgttgta ataggacatt cagaaagaag acaatacttc
    16441 aatgaaactg atgaaactgt aaacaagaaa gttttaaaag cattagaagt aggaatagac
    16501 ccaatattat gtgttggaga aactttagaa caaagagaag ctggaaaaac taaagatgtt
    16561 tgtaaagttc aagttgaaaa agctttagaa aatgttctta aagatgattt agcaaaagta
    16621 gttgttgcat atgagccaat ctgggctatt ggaactggta aaactgcaac tgctgaagat
    16681 gctaatgacg ttatatctta tataagagaa gttataaaag gattatatgg agaattagct
    16741 aatgaagtta gaatacaata cggtggaagt gttaaacctt caaatgtagc cgaaataatg
    16801 ggtcaaagtg atatagatgg agctttagta ggtggagcta gtttagctag taatgattac
    16861 ctagaccttg ttaatttcta aggagtgaaa tgatgaaaaa accagttgct ttaattatta
    16921 tggacgggtt tggatacaat aaggatgtta agggaaatgc aattgctgag tccaaaactc
    16981 ctaatttaga tagaataaaa aaagagtatc caaacacatt gataaatgct agtggtcttg
    17041 atgttggtct acctgatggt caaatgggaa attcagaagt tggacacact aacataggtg
    17101 caggtagaat agtatatcaa gatttaacta gaataacaaa atctataaaa gatggtgact
    17161 tttttactaa taaagtttta tgtgaagcaa tggataatgc aaaagaaaat tctcttcatg
    17221 taatgggact tttgtctgat ggaggagtac attcacatat agaccatctt aaggctataa
    17281 tcaaaatggc aaaagataaa ggcgtacaaa aggtgtatgt tcacgcattt actgatggta
    17341 gagatactga ccctcaaagt gcattagaat atgccaaaga agtacaagct agtatggatg
    17401 aaattggagt aggtgaattt gctacagttt ctggtagata ctacgctatg gatagagata
    17461 agagatggga aagagtagaa cttgcataca atgccatggt tagaggtatt ggtgaaaaag
    17521 caaattctat tgaggaagct attcaaaact cttatgatga tggaaaaaat gatgagttta
    17581 taatgccaac agttattatg aaagatgata aaccagctgg aagtataaag gaaaatgatt
    17641 caattatatt cttcaacttt agaccagata gagctagaca aataactaga gctttagttt
    17701 gtgaagagtt tgatggattt aaaagagaag atattaaaaa cttctttgta tgcttaacag
    17761 agtacgatat aactatagag aatgtgcata tagcatttgg acctcaatct ttagccaaca
    17821 ctctaggtga gtatctagct aaaaatggaa aaactcaact tagagctgct gaaactgaaa
    17881 agtatgcaca tgtaactttc ttcttcaatg gtggagtaga agagccaaat aaaggtgaag
    17941 aaagactatt gataccttct ccaaaggttg caacttatga tttaaaacca gaaatgtcag
    18001 catatgaatt gacagataaa gctttagata aattaggaga agataagttt gactttatag
    18061 tattaaactt tgcaaatcct gatatggtag gtcatacagg aagtatagaa gcggctataa
    18121 aggctgttga aactgtagat acttgtgtag gaaaattgat agataaaata gtagagttgg
    18181 gtggaagtgc aataataact gctgaccacg gaaatgctga atatatgtta gacccagaaa
    18241 caggaaaaac tgtaacggct cactctatta atccagtacc atttattgta gttggtcaag
    18301 aatatgaaag tgcaaaatta ttggatggag gaagattatc tgatatagca ccgactgttt
    18361 tagatatgat gaagttagaa aagccagaag aaatgactgg tcattcactt atttcaaaat
    18421 agtcgatttt gataagtaga gaataatata taaactacaa tataaattta aaaataatgc
    18481 agtttaggtt tattttgtaa agtttagact ttatgacata agatttaaat tttgtaagtt
    18541 ctggatattg taaggtgtag atgaagatat attttataaa atttacaaga tagacaattt
    18601 ataaaataaa aaattacaat atagataata atgtaaataa tgatataaat aataacaaga
//
LOCUS       DAGSRM010000079        18536 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.079, whole genome shotgun sequence.
ACCESSION   DAGSRM010000079 DAGSRM010000000
VERSION     DAGSRM010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18536
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.079"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            402..1805
                     /locus_tag="MJE33_002778"
     CDS             402..1805
                     /locus_tag="MJE33_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBY9648705.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEICYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
     gene            complement(2282..3304)
                     /locus_tag="MJE33_002779"
     CDS             complement(2282..3304)
                     /locus_tag="MJE33_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBY9648706.1"
                     /translation="MNQKKVHYNKSATSKDVARLAGVSQSSVSRAFGSANGKGVKPEV
                     RERIFRVANEIGYVPNLVARGMISGKTNVIGLIVGDSLGPFYNRIINLFVEKIQEIGK
                     QCLVFKVPRQQSIDSIISKVIQFQVEAVIITASAMNKVMAETCEENNIPVILFNRFIP
                     GIKISTVYVDPIEGGGMAAEYLLKKGHKNIGYIQFTRETSEEMEKKIGFYSKLRQSGI
                     HNLKEESANYDYDSGYEAGKRMLALSNPPTAIFCTSDLIAIGVMDAARFEYKLRVPED
                     LSVIGYDDIQMASWKSYNLTTIRQPLDLLIEKTINILKSLLNEENPESVVEMLKPELI
                     ERGSTL"
     gene            complement(3339..4937)
                     /locus_tag="MJE33_002780"
     CDS             complement(3339..4937)
                     /locus_tag="MJE33_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HBY9648707.1"
                     /translation="MISSIDVCIIIGYLLLMLVIGYYSGKDNKNQEDYFLAGRSMPWI
                     PIALSVAATMISANGFIGGPGWAYVDGMYPVMVNITVPLAIFFALSITTPVIYKLKIT
                     SIYEYMGLRLGNYSRGLTIIQFFINSLIQASSMVYIPVLIIQMITGWSLYALVPIVVL
                     VSIIYTLLGGIKAVIWTDSIQMIVVISAVFIVIIVALKGMNLSFFDTLQIAQQSGKLN
                     TFDFSRDISVTNTFWATLIGGTVMWIRYFCFDQSQVQRVLTSKSLKCAKNSFVVSAFV
                     MNLVYYFMLLVGVILFVFYGGRTFETSNEIMITFILNELPVGAIGIIIAGVFAAAMSS
                     IDSILNSMTTVFTKDIYEFYFKKDNKESSLKVTMIITVVIGVVMTGFIIMGFGGSIKS
                     ILDLVGNYISYFAGPATGAFVLAMFTYKANDKGVSIGFIVGLVLGFFISNKYNVSWLW
                     NPAIGATITLIFGYIFSVILKKNHNADDIKKYTAFGMRNHLISQGMDKVDGVSIVPFS
                     LGKQELIVLAFFIIQYIILFLIQF"
     gene            complement(4979..6244)
                     /locus_tag="MJE33_002781"
     CDS             complement(4979..6244)
                     /locus_tag="MJE33_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY9648708.1"
                     /translation="MKTTVEIENDIILGKDYSNLKRGIAFFSIALIYFFYCYNFMVGT
                     FIKPTMIYALADGGFGFSLKQTEEIFAVMSFGTIPGTFIFGVISTKIGKKRTLISVAL
                     LIGLTTFIPMLSPTNIMLWKIARLCTGVVLGGVFGTAMPLVADMFPSKYRGKLAAILT
                     ALFSLAMIFGGKVYGVLGDANWQILMYTAIIPPIVGAILAIFFVPDDLEYTKELVKKG
                     KETGEKISYISMYKGKYLWIGLGTILLSGANFTAYSAFSNNATTYLVTGIGMSAAVAG
                     SIYSLQGIGQLVGYLFWGSIADKFGRKIPFIGMALAAVFVFIYTKLGGDNTTTFYMIS
                     VLLGVAVGYSGAWGAYYTELFPSKYRSLSSGMSFNGGRIISTFAIPAIAGTASGTLGM
                     MTIFYISIAVFVAGAIVWLFLPETLNNKK"
     gene            complement(6388..7800)
                     /locus_tag="MJE33_002782"
     CDS             complement(6388..7800)
                     /locus_tag="MJE33_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HBY9648709.1"
                     /translation="MASTNKNNATSKEEEKEVSTSVLESCTVKEKKAKMNQTNFSNIN
                     ENQTNENKTNTYKGNIDKGNIDKTNTNKSDINKVNIDTVDTDKLNTNTLTEKNKKVIF
                     KGEDNMSSTKIYYGDSLEVTPVGVMDYNDFSKQTKREQEIPGFDSKYRDFVDYIMKIT
                     HNIWEEKGIGVIYDTYHNNVTMHCGSSNLVGIKDVISNTLQTLHAFPDRRLIGQNVIW
                     SNFGADGFLSSHRVLSTATNLGDSNFGPATGRKINFRTVIDCATTNNRIHEEWLVRDN
                     LWIVTQLGLNPQEVAKSMAKASESKVLSLQSTYGICESMDGQFMPTKYQAKDDSVGEM
                     MLEMTSRIYNYKYINEVKKYYHDNAVVHFICDKDLNGYDEIQGMIVSLLASIPNGSYE
                     VERVTCNQREKNEGYDVSVRWRLRGINEGIGFFGQPSGKHIEVMGINHYHILQGKVKE
                     EWITFDGMDVLKQMYMGVEE"
     gene            complement(7838..8845)
                     /locus_tag="MJE33_002783"
     CDS             complement(7838..8845)
                     /locus_tag="MJE33_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HBY9648710.1"
                     /translation="MGKYQYSKKVVRNYFEALENATGDEVENVLKQHMKSDYNWKGVY
                     PFREQEGTENVADVFWKPLKKSLSNMQRRQDIFIAGTNEIDNSEWVMSMGHFMGLFDN
                     DWLGIKHTRKMISLRYAEFNCVQDGKIAKTGLFVDIIGFMIQAGVNPLPPQTGSYFVY
                     PGPIDHNGLLFEDADEVEGVKTLALVNKMVDDLSELNESGAMGCPPEILEKSWAKDMI
                     WYGPGGIGASYTIPRYQEQHQLPFRNNLKGKTFNGHVCRFAEGNFACFFGWPNLTNVP
                     CGGFLGLPGGEVKADMQVVDVYYRKGDKLQENWVLIDIPYWLKQQGLDILERTKSIFN
                     A"
     gene            complement(9042..10364)
                     /locus_tag="MJE33_002784"
     CDS             complement(9042..10364)
                     /locus_tag="MJE33_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648711.1"
                     /translation="MKILYVGPGMVIDKILAVSKKNFPNIEADFLRYDRYIEVPDLLE
                     KYRGRVDAVLFTGKSPFKFFEKRQKDFVLSDYIPRHETTLYRVLLEITYLLKLDISRL
                     SIDTYDKAMMKRLYKEIGVEFKDEHMFFAEQKYLEEDYIKFVLEFHKYNYQNNDVCCC
                     ITGLEEAYHIMKKDNIPVVLALPSEDVMVQSIKNIQMRYIAKKNNENQIVVLAIKLNM
                     PSEYSLMKEDEYAYLSQRIKILDKLYHFNSRIDGVLVEQSRSEFMIFTTKKILELETK
                     KYRDIYLLDMIREVSVINTYIGIGYGKTANEAKFNAYESIKMAQSQKNDAAYVVFENG
                     EIMGPLKPNPTVKEKNSFDERFYQIATETGLSANTVYKVFNKIINSGKTEFTSKELAT
                     ICGVSIRTMDRIILRLCDSGYCEVVSEQLMSKYGRPSRILRFKPFLLF"
     gene            complement(10722..12362)
                     /locus_tag="MJE33_002785"
     CDS             complement(10722..12362)
                     /locus_tag="MJE33_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HBY9648712.1"
                     /translation="MFDVKIVNGIIVDGTGNSRYKSDVGIVGDKIMAIGDLSQKEAKE
                     TIDATGKFVSPGFIDFHTHSDLSLVYDKYTRSRIHTGVTTDVIANCGIGVAPIREEKK
                     QELIDYLGTRIIGTIPTKLELHWNTMQEYFDYLTENSPAVNVVAYVAQGPVRINEMGF
                     SKEPATLEQLKNMKLEVRKAMEAGCVALSSGLVYLPGAYTKKEEMAELCKELIPYNGY
                     YISHIRDEGDEEMEALDEAIYIAKTAGVPLHVSHLKVMGHKNFGTIDEVFKKLDEAEA
                     DGLEVTFDCYPYTAGMTSLGALLPPWAFEGGVENMVKRLEVQENRDRIIKELEEGIPG
                     WQCFYQLAGGWNGVVLASVMTEANKYVEGKTLMEVAKINGENPFDTFFRLLIEEKSKI
                     QVVVHTQGQEDTDKVVCHPKSCIGSDSMDLSTEGLLSLGKPHPRAFGTFGRIFSYYVR
                     EKGMLTFEEAVKKITYLSAKRLGIYKERGLLKENYFADIVVFDPDTIEDKATYSNPKQ
                     YTVGVEYVLVNGKIALAGGKQTDVCAGRVIKNPLSIAK"
     gene            complement(12467..13123)
                     /locus_tag="MJE33_002786"
     CDS             complement(12467..13123)
                     /locus_tag="MJE33_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648713.1"
                     /translation="MYDKKMIKIGRISIGLAIIANFIPAIYVGIRFGQMPMLSVIFQI
                     WGLVAATYGVSWIVQPIAYYPTLGAPGSYIGWLAGSVGDIRMPAASMAQKIAGVEAGT
                     HEGEVVGTIGTCCSIFVSASMITIFTIVGYKVIPLLPEFVTNSFNYILPALFGAIYVD
                     IARKDIRAGGCTIAVALAIMYFGEKIGIPGGLLTLFIVLGGILITRVFFVIDNNKTKE
                     "
     gene            complement(13149..13850)
                     /locus_tag="MJE33_002787"
     CDS             complement(13149..13850)
                     /locus_tag="MJE33_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HBY9648714.1"
                     /translation="MKFQEMINSPGLWIVSSFLVIISVVQAIVFMKEALKEASNLGIE
                     RKGIKAAIRSASVTAIGPSLSPVITLLSLVAVIGAPTTWMRLCDVGAARTELGVISLT
                     SNLSGVEVGSAAFGAEAFSYALWGMALNNLGWLFVVFILGHRMRGIVEKMNMKYNPTW
                     VKKLLAGATVGLFAYLLSNQIKTFEIPKLTPAIISAIVMLVLTTLFKKNQRLQELSLG
                     IAMLIGMFGTQIILL"
     gene            14658..15137
                     /locus_tag="MJE33_002788"
     CDS             14658..15137
                     /locus_tag="MJE33_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBY9648715.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAVNNAILKAYE
                     KLDSLKEEQYFKTWLIRIVINECNSLRRKRLKSLSFEDVFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRRLLKTKLENTEVII"
     gene            15134..15913
                     /locus_tag="MJE33_002789"
     CDS             15134..15913
                     /locus_tag="MJE33_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648716.1"
                     /translation="MNKNILNNSFPEVPESFHNSLSKTLNSLPEREENYKMINNKTHK
                     LPFRKGLVATLAITLILSTTALAVGHIYSLVGKSSTKATYTSIPTTEQVKKDFNFTPN
                     IVSEFSNGYKFKGAYTVNKKAFDKENNVLGKSKALSFEYKNGNDEISLDTANNVLGKS
                     SDNLKVVDTYKDINISYEAFAQKFVPEGYKMTEQDKIDEKSGKYTFSVGPNNESDKVE
                     VNKFKFLTWKQDEVYYSFTVQDSNLSMNDLVKMAHEVIDAK"
     gene            complement(16163..16663)
                     /locus_tag="MJE33_002790"
     CDS             complement(16163..16663)
                     /locus_tag="MJE33_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY9648717.1"
                     /translation="MIEGIKFIKAEEKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNIINKNLPQLFIIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSNLL
                     KQIIDLSKEYGYEKIELDVFKSNSRAIHVYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     MELTLI"
     gene            complement(17274..17981)
                     /locus_tag="MJE33_002791"
     CDS             complement(17274..17981)
                     /locus_tag="MJE33_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBY9648718.1"
                     /translation="MIFTKSNKYDKDFLMKNMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLAKEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISIDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDCIPDELIP
                     FWQDNMNFHSITWWNKLWSESESVIVEKCEALNCHDEAWKDWINCDNSYAINDKKMME
                     VENGKYFNTISLIARTK"
BASE COUNT         6427 a         3298 c         2137 g         6674 t
ORIGIN      
        1 aacaaatcct ataattatat tctaatatac tacctactac tttgcgctac aacaatttct
       61 ctatttatat gatttgtaaa atatagtaca acatttttta tcattttcat ttcaactaaa
      121 tttgtaatat tttattcaat acttaataaa tacttaatca atacatgctt tataatactt
      181 tactcaatat ataatcaata cataatttat aatttgtaat actttattca atacataagt
      241 agaaatctct atatacataa attcattttt tcatacatct tacaaattta tttttattta
      301 gataacatgt atattttttc catgaaaaat aaataataaa aaatagtaaa tatggaaaat
      361 ataaaattga ttaacaataa ttaattacct gaggtgtgaa tatgaaaatg ctaaacaact
      421 tttttaagaa aatcaacaat ccagctcctg tctttgcaca gacaaaaatg gaaagagata
      481 atcaaacaga tgattcactt tctacaaaac caaagactac atttagagac gttgctggat
      541 tagacgaagt aaaagaagaa ctatttgaaa tagtcgattt tatgaagtca ccacaaaaat
      601 atcaaaaaat gggtgcaaaa atacctaaag gagtattatt ttatggtcct ccaggaacag
      661 gtaaaaccct acttgcatct gctgtagctg gtgaaacaaa ctcatcattt ttcaatgtta
      721 ctggttcaga gttcgttgaa aaatatgtag gtgtgggagc aaaaagagtt agaactctat
      781 ttgaaaaagc tagaaaagaa gctccaagta taatatttat agacgaaata gatgctgtag
      841 gagcaaagag acatcttgaa agtaacaacg aaaaagacca aacactaaac caacttttag
      901 ttgaaatgga tggatttaat aaagattcca atgtattaat aataggtgct accaatagac
      961 ttgacttatt agatgaagct ttacttagac caggaagatt tgacagacac attcatatag
     1021 gtgcaccaaa ctatcacact agatttgaaa ttctaaaggt acatacagat gacaaaccta
     1081 tagacaaatc tgtaaatcta gaacttttag ctaaaaaaac tcatggtttc aatggggctc
     1141 atctttcaaa tatagcaaat gaagcagcta tatttgcagt tagagatgac agtgagtgta
     1201 ttacatcaga acattttgac aaggcacttg aaagagttat tgcagggcta gaatctaaaa
     1261 actctgcatt ggttgaaaaa gagaaaaaaa tagtagcata tcatgaagct ggacatgctc
     1321 ttgtaagtga tatagtagga atttgtccta tacaaaaaat atctatagtt cctagaggac
     1381 aagcattagg ttatgttctt caattacctg atgaagatag atatatttat actaaggatg
     1441 agttgattgg aaaaataaaa atattacttg ctggaaaggc ttcagaagaa ttaatattta
     1501 atcataaatc tactggtgca aaggacgact taaagaaagt cactgaaata gctaaccaaa
     1561 tggtttgtga atatggtatg agtaacttag gatttatgac tattgatgga aatgataaga
     1621 ctttcttatg tgataaggtt caaaaggaag caaatcgtat agttgaaatt tgttataaag
     1681 agactttaga gatgttaaag gataatcttg aagatttaca ttctgtttct aaattcttat
     1741 ttgaaaaaga aactatgact catgaagagt taaaagattt gataggaaaa gaagctgtaa
     1801 actaatatag ttttaaaaat ttgaggctgt ctcaaaatag agataagcct ctttttattg
     1861 ttaaagttat aaaaatacat ttgattttat acaaaaaagt gcctcataaa ctaaaaattt
     1921 cattttgaga cacttacata ttttaattac atattttaat tttgtctatt tacagaatat
     1981 tatatctaca taaaactaaa ctgctgtaaa cttaactacc tccagtaatg tttgaagtgg
     2041 tataaaagct agtagcctag tttctacact tataatttat attttacata actaaattta
     2101 gtagattttt atactcacaa tttagaacga ttgaaaactt aaaaatttct tatcagatat
     2161 ctacaactta attaggtatc tatcacttga ttaaatatct atcacttaat taggtatcta
     2221 tcacttgatt aaatatctgc catttaatta aatatctacc acttaattaa atatctacca
     2281 cttaaagtgt agaacctctt tcaatcaatt caggttttaa catttcaacg actgactctg
     2341 gattttcttc atttaataag gacttaagta tgttaattgt tttttcaatc aacaaatcta
     2401 gtggctgtct aatcgtcgtt aaattataac tcttccatga agccatttga atatcatcat
     2461 atcctatcac tgataaatcc tctggcaccc tcaacttgta ctcaaacctt gcagcatcca
     2521 ttacaccaat agctatcaag tcacttgtgc aaaaaattgc tgttggagga tttgataatg
     2581 ctaacattct cttacctgct tcatatccag aatcataatc atagtttgca gattcttctt
     2641 taaggttatg aatcccactt tgtcttaact tagagtaaaa tccaattttc ttttccattt
     2701 cttcacttgt ttctctcgta aattgaatat atcctatatt cttatgacct ttcttaagca
     2761 gatattcagc agccattccc ccaccttcaa ttgggtctac atagactgta cttattttta
     2821 taccaggtat aaaacgatta aaaagaatca caggaatatt gttttcttca caagtctcag
     2881 ccatcacctt attcatggcc gaagctgtaa taatcacagc ttctacttga aactgaatta
     2941 cttttgaaat tatactatct atactttgtt gtctaggaac tttaaaaacg agacactgtt
     3001 tacctatctc ttgaattttt tctacaaata aattgataat tctattgtaa aatggaccta
     3061 gactatcacc aactatcaat cctataacat tagtttttcc actaatcatt ccccgtgcta
     3121 ccaaattagg aacatatcct atttcatttg ctacacgaaa aattctttct cttacttcag
     3181 gttttacgcc ctttccattt gcactaccaa atgctcttga aacagatgat tgagatactc
     3241 ctgccaatct tgcaacatct tttgaggtag ccgatttatt ataatgaact ttcttttgat
     3301 tcatttagta tttctcctta aatatctagt atttcttctt aaaattgtat caaaaataat
     3361 ataatatatt ggataataaa aaaagctaat acaattaatt cttgtttacc taaagaaaat
     3421 ggcactatag atactccatc tactttatcc attccttgag aaataaggtg atttctcata
     3481 ccaaatgcag tgtatttttt aatatcatct gcattatgat ttttctttaa aatgacacta
     3541 aaaatatatc caaatatcaa tgttattgta gcacctattg ctggattcca caaccaactc
     3601 acattatatt tattagaaat aaaaaatcct aaaactaacc ctacaataaa accaatagat
     3661 actcctttat cattagcttt atatgtaaac attgctagta caaatgcacc tgtagctggt
     3721 ccagcaaagt atgagatata gttaccaaca aggtcaagaa ttgatttgat gcttcctcca
     3781 aatcccataa taataaaacc tgtcataaca actcctataa ctacagttat aatcattgta
     3841 acttttaatg atgattcttt gttatctttt ttgaaataaa actcataaat atccttcgta
     3901 aaaacagttg tcatactgtt taatatagag tcgatactag acattgctgc tgcaaacaca
     3961 cctgcaataa taattccaat ggctcctact ggaagttcat ttaaaataaa agtaatcatt
     4021 atttcatttg aagtttcaaa tgtccttccc ccataaaaaa caaataaaat tactcctacc
     4081 aatagcataa agtagtaaac taaattcata acaaatgcac taactacaaa agaatttttt
     4141 gcacatttta aagatttaga tgttaaaact ctttgtactt gagattggtc aaaacaaaag
     4201 tatcttatcc acattacagt tccaccaatt aaagtagccc aaaaagtatt tgtcactgaa
     4261 atatccctac tgaaatcaaa tgtatttaat tttccactct gttgtgcaat ttgtaatgta
     4321 tcaaagaaac ttagattcat tccttttaag gctacaataa ttactataaa tacagcactt
     4381 attactacta tcatttgaat actatcagtc catataactg ccttaatccc acctaataga
     4441 gtgtatataa ttgatacaag tacaactatt gggactaaag cataaagtga ccagccagta
     4501 atcatttgaa taataagcac tggaatatat accatagaac tagcttgaat caatgaatta
     4561 ataaaaaatt gaataatagt taatcctctt gaataatttc ctaaacgaag tcccatatat
     4621 tcatatatag aggttatttt taatttatag ataactgggg tggtaatgct cagtgcaaaa
     4681 aatattgcta aaggtactgt aatattaacc attactggat acattccatc tacatatgcc
     4741 catccaggac cacctataaa gccatttgca ctaatcatag tagctgcaac tgataaagca
     4801 attggaatcc atggcattga cctacctgcc aagaaataat cttcttgatt tttattatct
     4861 tttcctgaat aataaccaat aactaacatt aatagcaaat atcctattat aatacataca
     4921 tcaattgatg aaatcataag ccaccccttt ctaagtaaca aaataaattc ctagtatttt
     4981 attttttatt atttaacgtc tccggtaaga ataaccatac aatagcacct gctacaaaga
     5041 ctgcgattga tatgtaaaag atagtcatca tacctaaagt accagaagct gtaccagcta
     5101 tagcaggtat tgcaaacgta gatataatcc ttcctccatt aaatgacata ccagatgaca
     5161 acgaacgata tttacttggg aacaactctg tgtagtatgc tccccatgct ccagaatatc
     5221 caacagcaac acctagtaat actgatatca tatagaatgt tgttgtatta tctccaccta
     5281 attttgtata tataaaaaca aatactgctg ctaatgccat acctataaaa ggtatttttc
     5341 ttccaaattt atctgcaatt gaaccccaga ataaatatcc tactaattga ccaattcctt
     5401 gtaaactata tatagaccca gccactgctg ctgacattcc tattcctgtc actaggtatg
     5461 tagttgcatt atttgaaaat gctgaatatg ctgtaaaatt tgcacctgat aaaagaatcg
     5521 ttcccaatcc aatccataag tattttccct tatacatact tatatagcta atcttttcac
     5581 cagtttcttt tcccttttta actaattcct ttgtatactc caaatcatct ggcacaaaga
     5641 agattgctaa gatagctcca actataggtg gtataatcgc agtatacatc aatatttgcc
     5701 aatttgcatc acctaaaacg ccatatactt tcccaccaaa aatcattgct aatgaaaata
     5761 aagcagttaa tatagcagct aacttaccac gatatttact tggaaacata tctgcaacta
     5821 aaggcatagc tgttccaaat acgcctccca acactactcc tgtacacaaa cgtgctattt
     5881 tccataacat aatattggta ggagataaca ttggtataaa tgttgtcaat ccaatcagta
     5941 aagcaacgct aattaaagtc ctcttcttcc caatttttgt agaaatcacc ccaaaaataa
     6001 atgtacctgg aattgtacca aaagacataa ctgcaaatat ttcttctgtt tgttttaatg
     6061 aaaaaccaaa tcccccatct gctaaagcat aaatcattgt tggtttaata aaagtaccca
     6121 ccataaaatt gtagcaataa aaaaagtata ttaaagcaat tgaaaaaaat gcaattcctc
     6181 tttttaggtt tgaatagtct tttcctaata tgatatcatt ttctatttca accgttgttt
     6241 tcatattttt ataattcccc cttttaaatt tagtcataat tttgctaaaa gtttaaacat
     6301 ctatttttca tatacattcc ccacttaaat tttataaagc aatacttccc tatatacatt
     6361 tcaaaactat ttatactcta aacagcttta ttcttctact cccatataca tttgttttaa
     6421 tacatccata ccatcaaaag ttatccactc ctcctttact ttaccttgta gtatatgata
     6481 atgattgatt cccattactt ctatatgctt tccagaaggc tgtccaaaaa atcctatacc
     6541 ttcatttatt ccacgtaatc tccatcttac agatacatca taaccttcat ttttttctct
     6601 ttgattacaa gttactcttt ccacttcata acttccattt ggaattgatg caagtaaact
     6661 tacaatcatt ccttgaatct catcatagcc atttaaatct ttatcacata taaaatgtac
     6721 tactgcatta tcatgatagt attttttaac ttcatttata tatttataat tataaatacg
     6781 gcttgtcatt tcaagcatca tttcgccaac agaatcatct tttgcttgat acttagttgg
     6841 cataaattga ccatccatag attcacagat accataagtt gattgtaaac taagcacttt
     6901 agattcactt gcctttgcca tactctttgc tacttcctga gggtttaatc ctaattgagt
     6961 aacaatccat aaattatctc taaccaacca ttcttcgtgg attctattgt tagttgtggc
     7021 acaatcaatt acagttctaa agtttatttt tctacctgtt gctggtccaa aattactatc
     7081 acctaagtta gttgctgtag acaaaacacg gtgagaagat aaaaaaccat cagcaccaaa
     7141 gttagaccat attacatttt gaccaattaa tcttctatca ggaaacgcat gtaatgtttg
     7201 aagagtattg gaaataacat ctttaatacc aactaaattt gaagagccac aatgcattgt
     7261 aacattattg tgatatgtat cataaataac acctatacct ttttcttccc agatattatg
     7321 tgtaatcttc ataatataat ctacaaaatc tctatactta gaatcaaatc caggtatttc
     7381 ctgttccctt tttgtctgtt ttgaaaaatc attgtaatcc attactccaa caggcgttac
     7441 ttctaatgaa tctccatagt aaatttttgt agatgacata ttatcttccc ccttaaatat
     7501 aactttttta tttttttcag taagagtatt tgtatttaat ttatctgtat ccactgtatc
     7561 tatgttaact ttatttatgt cggatttatt tgtgtttgtt ttatctatat ttcctttatc
     7621 tatatttcct ttatatgtgt tcgttttatt ctcatttgtt tgattttcat ttatattact
     7681 aaaattagtt tgattcattt tagctttttt ttctttaacc gtacatgatt caagcacact
     7741 tgtagaaact tctttttctt cctctttact tgttgcatta tttttgttgg tagatgccac
     7801 tttatatccc cctttgtttt agaatacgta tgcataacta agcattaaaa atacttttag
     7861 ttctctcaag aatatcaagc ccttgttgtt tcaaccaata aggaatatca attaacaccc
     7921 agttttcttg aagtttatcg cccttacgat aataaacatc aacaacttgc atatctgctt
     7981 taacctctcc acctggtaat cctaaaaaac ctccacatgg tacattagtc agattaggcc
     8041 atccaaagaa acaagcaaaa ttaccttctg caaatctaca tacatgccca ttgaaagtct
     8101 ttccttttaa attatttcta aatggtagct ggtgttgctc ttgatatcta ggtatagtat
     8161 aagaagcacc aattccacct ggaccatacc aaatcatatc tttagcccaa gatttttcaa
     8221 gtatttctgg tgggcatccc atagcaccac tttcatttaa ttcagataaa tcatctacca
     8281 ttttatttac taatgctaaa gtttttactc cttcaacttc atctgcatct tcaaaaagta
     8341 atccattatg gtcaattggc cctggatata caaaatatga acctgtttgt ggtggcaatg
     8401 gattaactcc tgcttgaatc ataaacccaa ttatatctac aaataatcca gttttagcaa
     8461 ttttaccatc ttgaacacag ttaaattctg catatcttaa actaatcatt ttacgagtat
     8521 gtttaatgcc aagccaatcg ttatcaaaaa gtcccataaa atgccccata ctcattaccc
     8581 attcactatt gtcaatctca tttgttcctg caatgaaaat atcttgtcgt ctttgcatat
     8641 tactcaatga tttttttaaa ggcttccaaa atacatctgc tacattttca gtgccttctt
     8701 gttcacgaaa tggatacaca cctttccagt tataatctga tttcatatgt tgctttaaga
     8761 cattttcaac ctcatcccca gtagcatttt ctaatgcttc aaaataattt cttacaactt
     8821 tttttgaata ctgatatttt cccataatta tctcctccca gatttattta agttataaat
     8881 taactttctc atttcatgca tacgatttcc tttttaatga tttttcagct gttttaagtt
     8941 tattttatga atacatatgc atttttctaa atattaacat atatctaaaa aattgtaaag
     9001 aaaatgttat cataaaaatt taatttttga cttatatcta tttaaaacaa cagaaaaggt
     9061 ttaaaacgta atattctact gggtctacca tacttgctca taagctgttc actcactacc
     9121 tcacaataac cagaatcaca caatctaagt ataatcctgt ccatagttct aatacttaca
     9181 ccacatatag tagctaattc ctttgatgta aattcagttt ttccactatt tattatttta
     9241 ttgaatactt tatatacagt attagcactt agccctgtct ctgtagcaat ttgataaaaa
     9301 cgttcatcaa aactattttt ctctttgaca gtaggatttg gctttagtgg tcccataatt
     9361 tctccatttt caaaaactac atatgctgca tcattttttt gactttgagc catcttaata
     9421 ctctcataag cgttaaattt agcttcattt gctgtttttc cataaccaat tcctatataa
     9481 gtgtttataa ctgaaacttc acgaatcata tccaacaaat aaatatctct gtatttcttt
     9541 gtttctaact caagtatttt tttagttgta aaaatcataa attcacttct actctgctct
     9601 accaaaacac catctatacg gctattgaaa tggtatagtt tatcaagaat cttaatcctt
     9661 tgtgaaagat aagcgtactc atcttccttc attagtgaat attctgaagg catgttcagt
     9721 ttaatagcaa gaacaacaat ctgattctca ttgtttttct ttgcaatata acgcatctga
     9781 atgtttttta tactttgcac cattacgtcc tcagaaggca aggctaaaac tactggaata
     9841 ttatcttttt tcattatatg ataggcctct tcaagacctg taatacaaca acatacatca
     9901 ttattttgat aattatattt atgaaattca agcacaaact ttatatagtc ctcttctaaa
     9961 tacttttgct ctgcaaaaaa catatgttca tccttaaatt caactccaat ttctttatac
    10021 aatctcttca tcatagcctt atcataagta tctatactaa gtctactaat atctaattta
    10081 agcaaatatg taatttcaag caacactctg tacaatgttg tttcatgtct aggaatataa
    10141 tcagacaata caaaatcttt ttgtcttttt tcaaaaaatt taaatggcga cttacctgta
    10201 aaaagaactg catccactct acctctatat ttttccaata aatcaggtac ttcaatatat
    10261 ctatcatatc taagaaaatc tgcttcaata ttaggaaaat tctttttact tactgctagt
    10321 attttatcga ttaccatacc tggtcctaca taaagtattt tcatttcgtc tctcctttcc
    10381 taatgatact attatctctt aaaacatttt ctaaacctac catgactaaa gccacaggac
    10441 tcctgattca tagaattttg aataatcaat tatacctaat ataaaaagtt actttaagtt
    10501 ttttaattac gactatctgt tgaaacattt ttgtatttaa atttttgtat ttaaatctta
    10561 ttcattaata ttaattacat tttacaatta atatattgtt ttttctactt aaagttaaat
    10621 tctttagaaa acacaagtag agtaatcttt attaatttaa caaaaatagc cttccttaat
    10681 attcaaagaa agactatttt tataaaagtt cttaatgtat tctattttgc tatgctaaga
    10741 ggatttttaa ttacacgtcc agcacataca tcagtttgct ttcctcctgc caatgcaatt
    10801 ttaccattca ccaacacata ttccactccc actgtatact gtttaggatt actataagtc
    10861 gctttatctt ctattgtatc tgggtcaaat accacaatgt cagcaaaata attttcttta
    10921 agtagacccc tttctttata aatgcctaag cgttttgctg aaagataagt tatcttttta
    10981 actgcttcct caaaagtcaa catacctttt tctctaacat agtaagagaa aattcgtcca
    11041 aaagtaccaa atgcacgagg atgaggcttt ccaagagaaa gtaaaccttc tgtagaaagg
    11101 tccatactat cagaacctat acatgacttt ggatggcaaa caaccttatc tgtatcctct
    11161 tgaccttgtg tatgaacaac tacttgaatt ttactttttt cttcaatcaa aagcctaaag
    11221 aacgtatcaa atggattttc accattgatt ttggctacct ccattaaagt tttaccctct
    11281 acatatttat ttgcttcagt cattacagag gctagtacaa caccgttcca cccacctgct
    11341 aattggtaga aacattgcca tccaggaatc ccttcctcca actcttttat aattctgtct
    11401 ctattttctt gtacttctaa tcgttttacc atattttcta caccgccttc aaaagcccaa
    11461 ggaggtaaaa gtgcacccaa agatgtcatt ccagctgtat atgggtaaca atcaaaagtt
    11521 acttccaaac catctgcctc agcttcatct agctttttaa atacctcatc aatagttcca
    11581 aaattcttat gacccataac ttttaaatga gaaacatgta gaggaactcc tgcagtctta
    11641 gctatataaa ttgcttcatc aagagcttcc atttcttcat caccttcatc tctaatatga
    11701 gatatatagt atccattgta tggtatcaat tccttacata attcagccat ttcttctttc
    11761 tttgtgtatg ctcctggtaa ataaactaat cctgatgaaa gagctacaca tccagcttcc
    11821 atagcttttc taacttccaa cttcatattt ttaagttgct ctaaagtagc tggttccttt
    11881 gaaaatccca tttcattgat acgtacaggt ccttgtgcta cataagctac aacattgact
    11941 gctggagaat tttcagttaa gtaatcaaag tattcttgca ttgtattcca atgaagctct
    12001 aattttgttg gaattgtacc aattatccta gttccaagat aatcaattaa ctcttgcttc
    12061 ttctcttctc ttatcggagc aactcctata ccacagtttg caataacatc tgttgttaca
    12121 cctgtatgga tacggcttct tgtgtattta tcatacacaa gagataaatc agaatgagta
    12181 tggaaatcta taaaaccagg tgatacaaat tttccagttg cgtctatagt ctctttagcc
    12241 tctttttgac ttaaatctcc aatagccatg attttgtctc ctacaatccc tacatcactt
    12301 ttatatcttg aatttcctgt accatcaacg attattccat tgacaatttt aacatcaaac
    12361 atgctttttc tcctttcaag acttatttat attcatattt cttaataaac aatcctattc
    12421 ttttttctca acaaataatt ctagtctttc tccttaataa gcaatcctat tcttttgttt
    12481 tattgttatc aattacaaag aagacccttg tgataagtat cccacctaac acaataaaca
    12541 atgttaataa tccacctggt ataccaattt tttcaccaaa gtacataatt gctaacgcta
    12601 cagcaatcgt acatccacca gctcgaatat ccttacgtgc aatatcaaca taaattgctc
    12661 caaataatgc tggaagtata taattaaatg aattagtaac aaattcaggt aacaaaggaa
    12721 ttactttata gcctacaatg gtaaaaatgg taatcataga agctgataca aagattgaac
    12781 aacatgtacc aattgttcca acaacttcac cctcatgagt tcctgcttct acacctgcaa
    12841 ttttctgtgc cattgatgct gcaggcatac gtatatctcc aacagaacct gcaagccaac
    12901 ctatataaga acctggagct cctagtgtag gataataagc tataggttga acaatccaac
    12961 taaccccata agtcgcagcg actaaccccc aaatttgaaa tataacagaa agcattggca
    13021 tctgaccaaa gcgaatacct acatagatag cgggaataaa gtttgcaata attgcaagac
    13081 ctatagaaat tctccctatt tttatcatct ttttatcata cataattatc acctcttatc
    13141 cgttttaatc acaataatat aatctgtgta ccaaacatac caattaacat ggcaatacct
    13201 aaggataatt cttgtaatcg ttgattcttt ttaaataaag ttgtcaatac tagcataact
    13261 atagctgaaa taattgctgg tgtaagtttt ggtatttcaa atgtttttat ttgatttgaa
    13321 agaaggtaag caaataatcc aactgtagct cctgcaagta gttttttaac ccaagtagga
    13381 ttatacttca tgttcatctt ttcaacaatt ccacgcattc tatgacccaa aataaataca
    13441 acaaatagcc aacctaagtt atttaaagcc atcccccata gtgcatacga aaaagcttct
    13501 gctccaaatg cagcactacc tacttctacc ccagataaat tggatgtaag agatattact
    13561 ccaagctctg ttctagctgc tccgacatca cagagcctca tccaagtcgt aggagcgcca
    13621 atcacagcta ccagtgataa taatgtaata acaggagata gagaaggtcc aatggctgta
    13681 acactagctg agcgaattgc tgctttaatt cctttccttt caataccaag atttgaagcc
    13741 tcttttagtg cctctttcat aaatacgatt gcttgaacaa ctgaaataat tactaaaaaa
    13801 ctagaaacaa tccaaagacc tgggctatta atcatttctt gaaacttcat actttattcc
    13861 tccttaaaat aaatttttca gcattttatt gtgagctttc tttctgtaat cttgttatgt
    13921 ttttatattg ttttaattta ttttatctaa gatatagtat ctttaatagg agattatctg
    13981 tcttaaagtc tcactaaaaa cattaatact taaatatttt attcataatt agtaaatatc
    14041 agaaaattca catattattt ttttaacctg taattaactt ataaggtctt ttattatttt
    14101 gacatttcaa tataaaaatt ttaatcaaaa aactttatta ttttaaaaaa aattattatt
    14161 tttataattt atatttttta agtttattta tgttcatctc tatctaaaat aatgacattt
    14221 ctaactctta attctcattt aagtttcatt gttttttctt aaaagcctac ttttagtcaa
    14281 gatactgtta tctttactca ttatttaaag aacttatttt aaatctgtac atgaagtaaa
    14341 taatccaaaa gcattgttat tctaactact caacattaaa tacttactat tttctaatta
    14401 ctcaatataa acacttacta taaaactaaa tatataaatg gcttctaaag tttaatatat
    14461 ctacatggct ttatatatga tgggaaaata ctagaaaaaa atatattata gaatatttgt
    14521 atagaatagg caaaactttc aaacttaaat ttatcaaata atcataaaaa aagtgaacca
    14581 tctatagtaa aattttatct aataagtgaa aggcaaaaaa gtagcgctac gatttgcata
    14641 gaagggaagg aacttagttg gataaaacta catttacaaa caatatactt gaatcagaac
    14701 agactttgta tcgagtatct aagtctattc taggaaacga ccaagactgt gaagatgccg
    14761 tcaacaatgc tattttaaaa gcatatgaaa agttggattc tcttaaggaa gaacaatact
    14821 ttaaaacatg gctgattaga atagtcataa atgaatgtaa ttcattaaga cgcaaacgat
    14881 taaaatctct atcatttgaa gatgtcttca aaaacaaaaa aattgatgaa aaagatgact
    14941 atagcgattt gtatactgct attcaaagtc tacctaaaaa aattaaaatt ccaatagtcc
    15001 tttactatat tgaaggttat tcagtagatg aagttaagga aatattagat atacctcaag
    15061 gaactgtaaa aagtagatta tcaagaggaa gaagattatt aaaaactaaa ttagaaaaca
    15121 cggaggtaat catatgaata aaaatatatt aaataattcg tttccagaag ttccagaaag
    15181 ttttcataat agtttaagca aaacactaaa tagtttacca gaaagagagg aaaattataa
    15241 aatgattaat aataaaacac acaaattacc atttagaaaa ggcttagttg caactttagc
    15301 cattactttg atattgagca caacagccct tgcagtagga catatatata gtttggttgg
    15361 aaaatcaagt actaaggcta cctatacatc tatcccaact acagaacaag taaaaaaaga
    15421 ctttaacttt acccctaata tagtttctga atttagtaat ggatataaat ttaaaggagc
    15481 atatactgta aataaaaaag catttgacaa agaaaataat gttttaggaa aatcaaaagc
    15541 acttagtttt gaatataaaa atggcaatga tgaaatatct cttgacacag caaataatgt
    15601 acttgggaag agttctgaca atctaaaagt tgtagatact tacaaagata ttaatatttc
    15661 ttatgaagca ttcgcacaaa agtttgtacc agaaggttat aaaatgacag aacaggataa
    15721 aattgatgaa aaatcaggca aatacacatt tagtgtaggg ccaaataatg aatcagacaa
    15781 agttgaagtt aataaattta agtttttgac atggaaacaa gatgaagttt actattcttt
    15841 tacagttcaa gactcaaatt tatctatgaa tgacttagtt aaaatggctc atgaagtaat
    15901 agatgcaaaa taaacataag taataataag ttgtttatga gtttattatt tatgacttta
    15961 atttttttat tagtaaacat ggttgtatct tccatcttta tattttcttt aaatttaata
    16021 tataaaattt tagatattta gctaccttca aaagaaccat tattaaaaag cccttaaaaa
    16081 tatttctttt atatggaatt ttaagggctt taactttatt ttatacaagt gtatatggaa
    16141 ttttaaagac tttaacttta ttttatataa gtgtaagctc catttgaatt acttcttctt
    16201 ttacaggtct atcattccat gtaaaaccac tagatattgt atttacctct acaaacccta
    16261 aagatttata aacatgaata gctctactat tacttttaaa tacatcaagc tcaatttttt
    16321 catacccata ttcttttgat aaatcaataa tttgttttaa aagattactt ccgatacctt
    16381 tttccctata ctctttaaga atacccatgc ctaaataacc aactttttca gttttaggta
    16441 aaacgtcaca ccaaccaata cagttatctg attctaaatc aattataaac agttgtggta
    16501 agtttttatt tataatattt tttataaatt caactgtttc ttcaaaagga aaggcttcat
    16561 ccatagctag atatttcctc tcttttgcta ttttatcaaa agtttgccaa tacgaataaa
    16621 tatacttttc ttctgccttt ataaatttta tgccttctat caacttaaca tttccccttt
    16681 ttaatttgtg attgtagtta acttatttta tatgattcta gtttataatg ctatataaat
    16741 tactatatat tagcttaaaa tatctttgta tatttctata aaattaaaac caatatatct
    16801 atctcttgta tctctattaa aataattttt aatagtataa taaatatagc tctttaatct
    16861 tttgagtata tatacttaat cattttgaaa tttgatttaa agtataataa aatatataaa
    16921 aaaatatata aaatggtact ataaaatttt taatcttcat caataccttt tttgctttca
    16981 ttgttaaaac tattttttct attagtttat ttcttataaa acaaacattt agtattaaaa
    17041 tatatatcac acaatagtat ttgcaattta actttaaaaa ataaaaatac ccaactgacc
    17101 taagcaactc gattgggtat aaataatcta aaaaatcatt tttgtataaa ctctttattt
    17161 aacttacatt tttatttacc ttctacttta attcaactta tatttctatg cttcttatac
    17221 tctcattcaa cttatatttc tatgcttctt atacttttat aaattcatca caattattta
    17281 gttctcgcta tcaaagaaat tgtattaaaa tactttccat tctctacttc catcattttt
    17341 ttgtcattta tagcatatga attatcacaa tttatccaat ccttccaagc ttcatcatga
    17401 caatttaaag cttcacattt ctctacaatc acagactccg attcagacca aagtttattc
    17461 caccatgtaa ttgaatggaa gttcatatta tcttgccaaa atggaatgag ttcatctggt
    17521 atacaatcca cgaaatcttc ttttaatcca ggcattgcca tagcaagtat tcccccctct
    17581 tttacaagag gtgatatatg attgtctaaa aatccttctt tgtttccaaa atagtgataa
    17641 gaatcgatac ttatcaccgc atcaaagaaa ccttctgcat aaggtagttc atgtgcctct
    17701 gcctgtattg gaaatatttt atcatccaat ttaaactctt taaatctttt atagttttca
    17761 gttggttcta tccataaatc tgttgcaaat acagtagcat caaattcctt tgctaaaaat
    17821 atcgaagata tacctttacc acaacctaag tctaaaatac gcattccctt ttctagtttt
    17881 atcttacttg ttaattcttc caatattttt atacaattag gtcccatcat atttttcatg
    17941 agaaagtcct tatcgtattt attacttttt gtaaatatca ttctatcttt cctttcccta
    18001 tcagttatta tttattaaat tttatacgtt tgatttttta ttttttatat gcttcttata
    18061 ttgagcaatc tcttttttat tcattcttta acagacaatt ttaatgtgta attttatcca
    18121 ctctgtgata gtaactttaa tgaataatct tatctactgt gatagttatt ttagtgagta
    18181 attttatcta ctccacagca atcgctttaa tgagtaatct tatccactct atgatagtta
    18241 ttttagtgag taattttatc tactccacag taatcgcttt aatgagtaat cttatccact
    18301 ctataatagt cactttaatg agtaatcata atttttatat cattttagat acttactcta
    18361 ataagaagtc attttctaat acttaataag ttttaattac ttccacttta acctatatac
    18421 taattaactt atttaattta gtgcatattt gacactttac cttttattat accatttatt
    18481 atcataaata taataattct atttttttaa aataaatatt tataataatc taaaat
//
LOCUS       DAGSRM010000080        17859 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.080, whole genome shotgun sequence.
ACCESSION   DAGSRM010000080 DAGSRM010000000
VERSION     DAGSRM010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17859
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.080"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            73..375
                     /locus_tag="MJE33_002792"
     CDS             73..375
                     /locus_tag="MJE33_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY9648719.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEEAFFEEVLTVIEGIDK"
     gene            556..1962
                     /locus_tag="MJE33_002793"
     CDS             556..1962
                     /locus_tag="MJE33_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBY9648720.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPVITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFADPIYNKLGLNKMKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRINTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVIAMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGLSVVIYFVLWALFRKNKTSIL
                     DYLEKQALKNVEEEPVAV"
     gene            2306..3364
                     /locus_tag="MJE33_002794"
     CDS             2306..3364
                     /locus_tag="MJE33_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBY9648721.1"
                     /translation="MGDKMRASVLYNVGDVRYEMVDIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCGKCDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEEHILKLPDTLDYETAAGIEPATIAYHGIS
                     KSNIRVGDSVVVLGCGPIGQFVIQWAKVFGASKIIAVDIFDEKLELSKLLGANYILNS
                     KEVNVIKEIKKITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATECILRGELTLKGSWNSYTSPYPGRAWTATLDFMGKGDIIFKPMISHKIGLNEVGDF
                     LSKMSKREINFNKILVEI"
     gene            3463..4149
                     /gene="araD"
                     /locus_tag="MJE33_002795"
     CDS             3463..4149
                     /gene="araD"
                     /locus_tag="MJE33_002795"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBY9648722.1"
                     /translation="MLENLKEEVLKANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNIVDGKLRPSSDTPTHLVLYKEFEELGGIVHTHSSWATI
                     WAQIGKSIPSCGTTHADYFYGSIPCTRKMTREEIRNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNIMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            4167..4814
                     /locus_tag="MJE33_002796"
     CDS             4167..4814
                     /locus_tag="MJE33_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBY9648723.1"
                     /translation="MLDSLKGKVVEIAIKAEHYGLCKEKTGNFSIRDKDTGYILITPS
                     GVGREELKVEHICVLDLDGNVIEVEKGIKPSSEVLMHIEIYKTRENINAISHTHSLYA
                     TAFSVAKKPIIPIVYESVNYGGYVDIAPYERPGTLKLAKSVSKLLIDTDACLLERHGL
                     TTVGNDLDEALLKSRYVEEVAEIYYRSLVLNQFKELEVVDVDEFKSWKYPEEIKF"
     gene            complement(4987..6354)
                     /locus_tag="MJE33_002797"
     CDS             complement(4987..6354)
                     /locus_tag="MJE33_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HBY9648724.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRTKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLKSISNVATKIVPFMAV
                     IYVVICGIILISFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTVSLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(6544..6632)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            7060..9180
                     /locus_tag="MJE33_002798"
     CDS             7060..9180
                     /locus_tag="MJE33_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBY9648725.1"
                     /translation="MGKILVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVITWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKHLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKPIKRLWISSSTDKAIKDGFAKLKSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESTLKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSKICNRLLKSNIIANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEYESI
                     PNVLEKDVLKVKGLKITSGKTNPPPYLNEGTLLTAMEKNGLGTVATRADIIEKLFNSF
                     LMEKKNKDIYITSKGRQLLDLVPVDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRIFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQNKNQETFNNPFAEALAKLKNK"
     gene            9284..10537
                     /locus_tag="MJE33_002799"
     CDS             9284..10537
                     /locus_tag="MJE33_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HBY9648726.1"
                     /translation="MDLSGIFKYYCKECENTWNNSSVELFENIETYSKDSQKKREKEL
                     DKLLNTISVHLERYPSDAVLRKMWVKKGEVFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDDDLPIGDIMQAMRNVWISNALQLLFGKEVYYSKANFAYSMLYPYTD
                     NYLDNTNIDKNDKILFNNWLEKRLLGEHIKSKDYHESKVSQMIDYIESVYPREKFTEV
                     YESLLLIFKSQVNSLKQHGKENHLCKEDLLSISIEKGGSSVLVDGYLISGLMTKEEIE
                     FCIGYGFLLQISDDLQDIKEDLKYNHKTIITEMSKEGTLDKVVNKLINFTIELIDSFK
                     INNKNKSVITMIKNDCLMLILFSVVYNAEFFSVGYIKEVEKFIPYTIDYSLEIEEKIK
                     EKFKNIDVLNNENEYKEMIDIICAE"
     gene            complement(10691..11752)
                     /gene="ytvI"
                     /locus_tag="MJE33_002800"
     CDS             complement(10691..11752)
                     /gene="ytvI"
                     /locus_tag="MJE33_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBY9648727.1"
                     /translation="MDIQKQKNFIIKFTYVVLITSIVYIVLKFLLPLLMPFVISFIIA
                     SILRPIIRLITNNTNLNRTLISIVILLIFYGLCIFLLVSFGVKIFASVSDVFFRLPEI
                     YKSSIQPTLNTLFSKIDASTPNVNLALILGWDNISQSMMSLVASISTNALNAIASIAS
                     KTPAFMLKLIITLIASFFFTFDYQKIVNFILKQFPEKSQFMIINIKNSSINALLKLLK
                     AYAILLSVTFIELLIGLTILKVENAFTISVIIALVDILPVLGTGSILTPWMIISLING
                     NINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPLITLMCMFVGAQLFGIAGLFGFP
                     IAATIIKNLHDNGIITAFK"
     gene            12026..12766
                     /locus_tag="MJE33_002801"
     CDS             12026..12766
                     /locus_tag="MJE33_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HBY9648728.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILRELN
                     IKYIFDYRSDEEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIEYKPKFKDIPKENLKYIFGVNEEYMKTAFRRIDEE
                     YISVEAYLYGEFNLNKEEIRKLRNQYLE"
     gene            12778..14556
                     /locus_tag="MJE33_002802"
     CDS             12778..14556
                     /locus_tag="MJE33_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBY9648729.1"
                     /translation="MSLNITARQINIIKILLNSKDTISGIALSQEIGCSSKTIQNEIK
                     DINKQLRNGKILSIRGVGYKLEGSFDEVNLNSNLYDDVDRVEYIIRKILTLSNEEKNT
                     IKLEDLADSMYVSVSTVKNDLKEVKRMLEKYSISVKSKHKQGICVLESEDKILNCIVD
                     LSNKRDNQLSLNDFLNDDIKERKLLIKKLLLDILNENGLVLTDIEFKSVLNNILVMLS
                     RHKYNEKEFIENYIENYRSKRSSILENEQNKEIIINSIKTFCKNLKLATSIDISNDKV
                     FEEFLYKHISSLCKKIKLGINQSPIMAQDIKIKYPFAFELANIAKKTIEKDLKIEINE
                     DEVANIALHIGGAVERASYNNKDKVFKTIIVCTSGIGTSMLIKAKLENIFKEKLEILK
                     IIPSYLVEYIKVIDVDFVISTVPIEVGDVPVINISPILSEKEIRLIEKYIETGNIYLD
                     LDIKSLFDSELFFTDLVFKRKEEVIDFMASKLVQKGYIDEDMKKSYFEREKIATTEIG
                     NMVTIPHGAIGKIFDNKIAIGILKKPISWEISDVRLIIMLALDKDKILDYEVIFSKIY
                     KRVDSIAKVISICENSSFEKFVNLFN"
     gene            14723..15640
                     /gene="pfkB"
                     /locus_tag="MJE33_002803"
     CDS             14723..15640
                     /gene="pfkB"
                     /locus_tag="MJE33_002803"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBY9648730.1"
                     /translation="MIYTVTLNPSIDYIVKLDELKTGSTNRVKEEYVYPGGKGINVSR
                     ILKELGNDNTSLGFISGFTGEYIIRTLEEKELKTDFIKIKSGFSRINVKIKALKETEI
                     NGQGPNIDDEDIDTLYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNDELEELFGVKLNSIEDMVKYARRLKEMGAINVLVS
                     MGKDGALLITEEDEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKQF"
     gene            15677..17596
                     /locus_tag="MJE33_002804"
     CDS             15677..17596
                     /locus_tag="MJE33_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY9648731.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKSEAIEELVDLVANSGNLNDKENY
                     KKAILAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSSSEKEFKEIIDKKEREKFSEEYQD
                     EKVTEKNIIDTKENDANKYPKVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVEMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YTINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMVGFLNGLGGTSKIVLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
BASE COUNT         7115 a         1947 c         3122 g         5675 t
ORIGIN      
        1 gtattatttt tttaataggt ttaatcttat ataaaataaa aatttagagt gaaaaattag
       61 gaggaaatta ttatgaaaca atttaatgta ttatcagttt gtggttcagg gacagttaca
      121 tcatctatgg tagcaggtaa attaaaagaa gtactaggta ataaggggat ttctattact
      181 acaacagaag ctagaccaac agaagcacta aatttagcac aatcaggaag atttgacttt
      241 ataacattta cgagtccact tcaaacaggc gattatggaa taccaactat aaatgcattt
      301 gcttgcctaa ctggtatagg ggaagaggca ttctttgaag aagttttgac tgttattgaa
      361 gggatagata agtaagtttt ggagacgtga atttatagta tttaaatcaa tgatttagat
      421 actataataa gctaatatta actaaatatt tgtggaaaaa acattttata tgttgtcata
      481 aattaaaaaa ttataaaaat agtcacttaa gtattaaatt aagcttataa atatttataa
      541 gtaggaggga aaaagatgtt agaatttatg aaatctataa tcgatacatt tggttctgca
      601 attatagttc caattataat attcataatc gcaaagatat ttaaagtaac tactaagaaa
      661 tcgtttcttt ctgctgtgta tgcaggagtt gctttacaag gttttacact gattttaaat
      721 tcatttacac cagtaataac accagttatc aataggatgg ttgagagtac aggagtaaac
      781 ctaccagttt ttgatgttgg ttggcaagca acatcattgg ttgcattttc aacaagtgca
      841 ggtatgatat atttaggtct tggtatatta ttacaaacaa ttttattttt gataaaatgg
      901 acagatgtgt ttcaaccatc agatttatgg aataactatt cttatatggt atggggagca
      961 atggttatag gagtaacagg aaattttcct ttaggtatag catgtatggt tttattaaac
     1021 ctatatagtt tattaatatc agagttagtt gcaaagagat ggtctagtta ctatagatat
     1081 cctaattgta caataatagc tatgcataat gttgaagctt ctgtgtttgc agtatttgca
     1141 gaccctatat acaataaatt gggattaaat aaaatgaaat taaatccaaa agaattagag
     1201 aagaaattag ggtttttagg tgaaccaatt actttaggtc tttttttagg tatgtttata
     1261 ggtatattag gtaatatgac gagaataaat actatggaag cttggggaga aataatgaaa
     1321 gtaggtatat ctacaagtgc agtgatggca attttcccta aagtagcaag tatgtttgct
     1381 caagcatttg cacctataac agaagcagct agaaagatta tgcaaaaagc tggaaaccgt
     1441 gagtggtaca tagcagtaaa tgatgctgtt ggatatggtg aacctgcaac tttaatatca
     1501 ggtcttatat taattccaat aatgcttgta atagctatgg tacttcctgg taataaggta
     1561 ttaccagttg tggatttatt ggcaattcca tatatggttc aaggtttagt tgccatacat
     1621 aatggtaata taccaaaagt attagtttca ggtataattt ggtttggatt aggattatat
     1681 gtttgtacat ctacagcacc attatttaca gatatggcta caaatatagg tgtagctata
     1741 cctgcaggag caatgttaat aacaagtttt aatatactag gaaaaccttt gatgggatta
     1801 gttttctttg catttctaag tgcaaatcca atttatatag gtctttcagt tgtaatttac
     1861 tttgttctat gggcattgtt tagaaagaac aaaacaagta ttcttgatta tttagaaaaa
     1921 caagcactta aaaatgtaga agaagagcct gtagcagttt aagtttataa aaatgaaata
     1981 aaaataatta aaggagttta taattgattt tatataagta tcagataata cctgagaaat
     2041 atttaaatga aatatagact tctttttatt tgataatatt tgtaattata atgcattgta
     2101 tctttatagt tgagttttct gtataacctg ctatttaaaa catactcttt tatatttata
     2161 cagtaatgtg gtttaatttt ataaaatgta atctctactg gtaaaagact ttgtatataa
     2221 ggcatttgcc tgaataaacc atttatcttt tgtatggcat agatattgct atatagaagt
     2281 atatagtatg aaaggagata tttatatggg agataaaatg agagcatcag tattgtataa
     2341 tgtaggtgat gtcagatatg aaatggtaga tataccagaa ataacagata cgcaagtgct
     2401 tgtaaatgta aaatatgttg ggatatgtgg ttcagattta cctaggtcta tggtaagtgg
     2461 tttgagtgga aatacaaaat atccattgat tttagggcat gaattttctg gtgaagttgt
     2521 taaaattgga gaaaaagtaa agcatattaa tgtaggagat agagtagctg ttgcaccatt
     2581 agtaccttgt ggaaaatgtg attattgtaa tgaaggtaat tttggcttgt gtgatgatta
     2641 caatataatt ggaacaagag taaatggagc gtttgcagaa tatgtaagag ttccagaaga
     2701 acatatttta aaactaccag atacattgga ttatgaaact gctgccggaa ttgaaccagc
     2761 aactatagct tatcatggga tttcaaagtc taatattaga gtaggagata gtgtagttgt
     2821 gttaggatgt ggtccaatag gtcagtttgt tatacaatgg gcaaaggtgt ttggtgcatc
     2881 taagattata gcagttgata tttttgatga aaaattagaa ctttctaaac ttctaggagc
     2941 aaactatata cttaactcaa aagaagtaaa tgtaatcaaa gaaataaaaa aaataacaaa
     3001 tggtggtgct gatgttgtaa tagaaactgc tggtagccga tttacacaag aacaatctct
     3061 atttgtagca aaaaagagag gaaatatagt gtttgtaggt atatcacaca cagaattacc
     3121 acttagtgca gacgctactg agtgtatatt acgtggagaa cttaccttga aaggttcttg
     3181 gaattcatat acaagccctt atcctggaag agcatggact gcaacattgg attttatggg
     3241 aaaaggtgac attattttta aacccatgat ttcacataaa ataggtctta atgaagtggg
     3301 tgacttttta agtaaaatga gcaagagaga aattaatttc aataaaatcc tagtagaaat
     3361 ataaaaattt aataaaaaat tctaaaagca attaaatttt ataactatga cattaaataa
     3421 aataaaaata attttcttaa ataaaatata ggaggaagaa aagtgttaga aaatttaaaa
     3481 gaagaagtat taaaagcaaa cttagaatta cctaagaaaa agctaattac ttatacttgg
     3541 ggaaatgtaa gtgggataga tagagaaaaa ggattagtgg tgataaaacc aagtggtgta
     3601 gagtatagta atatgactgt agaagatatg gttgtagttg atttagaggg aaatatagtt
     3661 gatggcaaat taagaccttc ttcagatact ccgactcatc tagttttata taaagagttt
     3721 gaagaattgg ggggaatagt acatacacat tcaagttggg caacaatttg ggctcaaata
     3781 ggaaaatcta taccatcttg tggtactact catgcagatt atttttatgg aagtatacca
     3841 tgtaccagaa aaatgactag agaagaaatt cgcaatgaat acgaaaagga aactggaaat
     3901 gtaatcattg aagaatttat gggaagaaat ccaattcatt gtccaggaat aattgtaaat
     3961 gaccacggac cttttacatg gggaaaagat gcaaatgagg ctgtacacaa tgcagtagtt
     4021 ttagaagaag tcgcaaagat ggcttattat acagagttaa tgagtcctga taatattatg
     4081 gataaggttc ttatgaataa gcatttttca agaaaacatg gaaaaaatgc atactatggt
     4141 caaaaatgat tatctggagg tgaattatgc tagactcttt aaaaggtaaa gttgtggaaa
     4201 tagcaattaa agcagagcat tatggtttat gtaaagaaaa gactggtaat tttagtatta
     4261 gagataagga tactggatat atacttataa ccccatcagg tgtaggaaga gaagaattaa
     4321 aagttgaaca tatttgtgta ttggatttgg atggaaatgt aatagaagtt gagaaaggta
     4381 ttaagccaag tagtgaggta ttgatgcata ttgaaatata caaaacaaga gaaaatataa
     4441 atgccatatc acatactcat tctttatatg caacagcatt ttcagtagca aagaaaccaa
     4501 ttataccaat tgtatatgaa tcagttaatt atggcggtta tgtagatatt gctccatacg
     4561 aaagaccagg aactttaaaa ttagctaaga gtgtttcaaa attactgata gatacagatg
     4621 catgcttatt agaaagacat gggttgacaa ctgtaggtaa tgatttagac gaagcattat
     4681 taaaatcaag atatgtggaa gaggtagcag aaatatacta taggtcttta gtattaaatc
     4741 aatttaaaga acttgaagta gtagatgttg atgagtttaa atcttggaaa tacccagaag
     4801 aaataaagtt ttaatctaaa taattttcat tgaagtggtt aattaaaata tatgtattat
     4861 ttatatcaat tactatgttt tattatgtta attaccgtat tttgcaaata tatttattac
     4921 attttttcaa aaaaatatag aatccgaaag ccatataaaa ttgacttggg attctatact
     4981 ttttatttat gcatttttct ttatctgatt ttctggagaa tttatatgtt caaaatattt
     5041 tttagtttca gatactatta taggtgataa tgcaagtaaa gctataaggt taggtattgc
     5101 cataagccca tttacaatat cagcaattat ccaaacaact tctaacttta agaaagcacc
     5161 taataacacc atagccacaa atacagttct atacccattc atgcctttta caccaaacag
     5221 aaattcaaaa catctttctc cataataact ccatcccaaa attgtagtaa atgcaaataa
     5281 tgttaaacta actgtaagta atattggtcc aaatgtagga attgctccgt taaaagctgc
     5341 ttgtgtcata acagctccat ttaggtctga acaccacact cctgaaataa caagagagaa
     5401 tccagtaaga gtacagatta taatagtatc tataaaagta cctgtcatag atataagacc
     5461 ttgttccgct ggccattcag ttttagcagc agcagcagct attggagcac tacctagacc
     5521 agactcattt gaaaatacac cacgagcgat accatttcta attgctagtg acatagttgc
     5581 tcctaaaaaa ccaccagtag cagcagtagg tgtaaatgca cttttaatta ttaacataaa
     5641 agtttcaggg attttattcc aaaaagatat taaaatgatt ccacagatta caacatatat
     5701 aactgccata aaaggtacta ttttagtagc tacattagat atacttttaa gtcctccaaa
     5761 tacaactaaa gctacaaata ctgctaatac aacccctgta attctagggt ctatcccgaa
     5821 actattattt attgcatctg ttattgaatt tacttgagta aaagtaccga ttcctaaaag
     5881 tgcaactagt ataccactta tagcgaaaaa tattgcaaga ggtctccatt tttctcccat
     5941 accatttact atgtaataca ttggcccacc agacacttga ccatttttat ctttagttct
     6001 gtattttatt gcaagaacac cttctgaata cttagtagcc attccaaaga atgctgctat
     6061 ccacatccaa aaaagtgcac caggtccacc agctttgata gctgtagcaa caccaacaat
     6121 attaccagta ccaactgtag cagctagagc agtacataag gctgcaaaac ttgaaacatc
     6181 accactacct ttgttttcag cagagaatat taacttaaga gctcttggca gtttagttat
     6241 ttgaacaact ccaagcttaa cagtaagtag tataccagta ccaactaata gtaccagtag
     6301 gggtggtccc caaataaaag catcaacttt gtttaaaata tcaattagat tcataaaaac
     6361 actccctcat tttttttgct ttacataatc aatagaattt ttaatgaagt aattgacagg
     6421 aaataagtat aatttattaa taatttctag tgtaataaaa aaagctaaat ctaaaaaaga
     6481 ttcagctctg gatgtacact aattagcaac caaaataaaa gtattagtaa atactgtttt
     6541 tggtagataa tccctgtcct tttgcctgag agattgagca taaaagcctt gccccttcgg
     6601 cgcccataat gggtctctcc agagttccgt aaattttaca gtcctacttt taaaagtacc
     6661 tgagagtgtt actccttcgg tgtgctatat agcaactctt ctataaaatc ttaaccggat
     6721 attgatttgt catagcttat tgtattaata atattaatat aagtttttat gttttgtcaa
     6781 ctaaaaaatt aaaaaacaaa gaaaaaatat aaaaatgtaa gcctatttac gaattataaa
     6841 cttagaaaga tgagtaatat acgtgaaaat gaattttgta atacacatac aaatgaagtg
     6901 taaaataagt tttaaataaa aaaataataa aatcttaaat aattaattag atatgattaa
     6961 attatggtaa tattatagtt acgatattaa gaaaagttct atttagttag attttaagcg
     7021 taatatgaga ttatgtatag agaaaggatg tatttttaga tgggaaaaat attagtgcta
     7081 gcagaaaaac cttctgttgg aagagatata gcaaaagttt taggatcaaa aaatgaaaaa
     7141 aatggttata tagaggggcc aaagtatgtg ataacttggg cactaggtca tttggttaca
     7201 cttgctgacc ccgaaagtta tggagaaaga tataaatcat ggagcttaga agacctgcca
     7261 atacttccca agcaccttaa gactgttgta ataaaaaaga gtggaaagca gtttaataca
     7321 gttaaatcgc aaatgaatag aaatgatata gatgaaatag ttatagcaac agatgcaggt
     7381 cgtgaaggag aacttgtagc aaggtggata atagaaaaat cacaggtaaa aaaacctata
     7441 aaacgtcttt ggatttcttc aagtacagac aaagcaataa aagacggatt tgcaaaatta
     7501 aaaagtggaa aagaatatga aaatttgtat tactcagcaa tagcacgagc agaagctgat
     7561 tggattattg gaataaatgc aaccagagct ttgacaacaa agtacaatgc acagctttct
     7621 tgtggaagag tacaaactcc tactcttgca atgttgctta aaagagaaga agaaataaga
     7681 aatttcaaac ctaaagagta ttatggatta gaattaattg ctacaaaggg aaattcagat
     7741 ataaaattta tttggaatga taaaaacaat aattcttcaa cttttagtaa agaaaagata
     7801 gaatccacat taaaaaaagt aaaaggtgta gacataaaaa tatctgaagt aaacaagtca
     7861 tataagaaaa aatattcacc tgcactctat gatttaacag aattacaaag agatgcaaat
     7921 aaaatgtttg gattttcagc aaaagaaact ttatctataa tgcaaaaact gtatgaacat
     7981 cataaagtac ttacttatcc tagaactgat tctagatatt taacatcaga tattgttgga
     8041 acattaaaag atagaataaa agctgtaaat attagtgatt attcaaagat ttgtaataga
     8101 ttattaaaaa gcaatattat agctaataaa tcgtttgttg acaattcaaa ggtaacagac
     8161 caccatgcaa taataccaac agaacagaag gtattcatga gtgattttag tgataaagag
     8221 cgaaaaatat ataatcttgt agtaaaaaga tttttagcag tactatctcc tccttttgaa
     8281 tatgagcaga caactataag tgcaactgta gaaggagaaa tatttaaggc taagggaaac
     8341 aaagtaataa atttaggttg gaaagaaagt tataaagata ttgaagatga agatgagtat
     8401 gaaagtatac caaatgtttt agaaaaagat gtacttaagg tcaagggatt aaagataact
     8461 tcaggaaaaa caaatccacc accatactta aatgagggaa cattattgac tgctatggaa
     8521 aagaatggtc tagggacagt tgctacaaga gcagatataa tagaaaaatt attcaattca
     8581 tttttaatgg aaaagaaaaa taaagatata tatataacat caaaaggaag acaattatta
     8641 gatttagttc cagtagactt aaagacacca gaacttacag cttcatggga aaagaaatta
     8701 ttggatattt caaagggaaa acttagtaaa aatacattca taaatgacat gaaagattat
     8761 tcaaaagata ttgtactaga agtaaaaaat agtgataata aatttaaaca tgataactta
     8821 actaaaaata gatgtccaga atgtggaaaa tttatgttag aggtaaatgg taaaaaaggt
     8881 aagatgttga tttgtgaaga tagagaatgt aatactagaa aaactatatc acaagtaacc
     8941 aattcaagat gtcctaactg tcataaaaaa ttagaacttc gtggagaagg tgaggggaga
     9001 atcttcactt gtagttgcgg atacagagaa aagttgtctt ctttcaacaa gagaaagcaa
     9061 gaagaaaaac agaaggcatc aaaaaaagat gttaatcaat atttaaaaaa tcaaaataaa
     9121 aatcaagaga catttaataa tccatttgca gaagcattag ctaagctaaa aaacaaataa
     9181 ttttaaagtt attattatac acaattataa ctttaactgt atattagtta tatgtactga
     9241 tataatatag tgtatagttt aggcatgaaa gtagggctgt taaatggatt taagtggcat
     9301 atttaaatac tattgtaaag agtgtgaaaa tacatggaat aattcgagtg ttgaattatt
     9361 tgagaatata gaaacgtata gtaaagattc acaaaaaaag agggaaaaag aattagataa
     9421 attgctaaat acaatatcag ttcatttaga gaggtatcca agtgatgctg tattgagaaa
     9481 aatgtgggta aaaaagggcg aggttttctt acaaaagaca ttggaaaaag aaaatatttt
     9541 taagttagaa aaaatggatg tagaggatag aaaaaaattt ttagatataa caaaacagtt
     9601 tattagagat gctagaaaat ttgatgatga tttacctata ggtgatatta tgcaagctat
     9661 gaggaatgta tggatttcaa atgcattaca attattattt ggtaaagaag tatattattc
     9721 aaaagctaac tttgcatata gtatgttata tccatatact gataattatt tagacaatac
     9781 aaatatagat aaaaatgata agattttatt taataactgg ttagaaaaaa ggctcctggg
     9841 agaacacatt aaatctaagg attatcatga aagtaaagta tctcaaatga tagattatat
     9901 tgaaagtgta taccctagag aaaagtttac agaagtttat gaatcgttat tattaatatt
     9961 taaaagtcaa gtaaatagtt taaaacaaca tggtaaggaa aatcatttgt gtaaagaaga
    10021 tttattatcc atttctattg aaaaaggagg ttcatccgtt ttagtagatg gatatttaat
    10081 aagtggattg atgacaaagg aagaaataga gttttgtata ggatatggat ttttattaca
    10141 aatatctgat gatttacaag atataaaaga agatttaaaa tacaaccata aaactattat
    10201 tacagagatg tcaaaagagg gtactttaga taaagttgta aataaactaa taaattttac
    10261 tattgagtta atagatagtt ttaaaattaa taataaaaat aaatctgtaa taactatgat
    10321 aaagaatgat tgcttaatgt taattttatt ttctgtagtt tataatgctg aatttttttc
    10381 tgtaggatat ataaaagaag tagagaaatt tattccatat acaatagatt attcattaga
    10441 gattgaagaa aaaataaaag aaaaatttaa aaatatagat gttttaaata atgaaaatga
    10501 atataaagaa atgattgata ttatttgtgc agagtagaaa aaaagggagt gtcttagaat
    10561 agacttttta gtttatgagg cactcttttt gtataaaatc aaatgtattt tcatcatttt
    10621 aacaattaaa aagaacttat ccctatttga gaaagccctt tttaatttaa ataaaaatgt
    10681 caaggttaaa ctatttaaat gcagtaataa tcccattatc atgtaagttt tttatgattg
    10741 tagctgcgat agggaatcca aataatccag ctattccaaa tagttgagcc ccaacaaaca
    10801 tacacattaa tgttattaaa ggatataggc caatttggtg tcctactacc tttggttcta
    10861 atatttgtct aacaacggtt ataattatgt atagtattaa aagtcctatt gctaaattaa
    10921 tatttccatt aatgagtgag ataatcatcc aaggtgtcaa aatacttcct gtacccaaga
    10981 ctggcaaaat atcaacaagt gctatgatga cagatatagt aaatgcattt tcaaccttta
    11041 ggatagtaag tccaataagt agttctatga atgtcactga tagtagtatt gcataggctt
    11101 ttagtagttt taaaagagca ttaatactgc tattttttat atttattatc ataaattgac
    11161 ttttttctgg aaattgtttt aagataaagt ttactatttt ttgatagtca aaagtaaaga
    11221 aaaaagaagc aattaaagta ataataagtt ttaacataaa tgcaggtgtt ttacttgcaa
    11281 tactagcaat agcattgagt gcatttgttg aaatagaagc tactaaagac atcatagatt
    11341 gacttatatt atcccaacca agtattaatg ctaggttaac attaggtgta ctagcatcta
    11401 tctttgaaaa taaagtattt agcgttggtt gtatacttga tttatatatt tcaggaagtc
    11461 taaaaaatac atcacttaca cttgcaaata tcttcacacc aaagctaact aataaaaata
    11521 tacacaaccc ataaaaaatt aaaagtatta ctatagaaat aagtgtacga tttaaatttg
    11581 tattatttgt aataagtctt ataataggtc ttaagatgga tgcaatgata aaactgatta
    11641 caaaaggcat taataatggc aataagaatt ttaatacaat atatacaata cttgttatta
    11701 aaactacata tgtaaattta attataaaat tcttttgctt ttgaatatcc ataatataaa
    11761 acctccaatg tatttgatat tattctaaat atggtctttt ttacagaaaa tatactactg
    11821 tatattgatt ataaactata aagcttatgt aaagtaaata aattatgtaa aatttaggtt
    11881 aattttgtat aaagagatac ttttatattt tacaattaat aaccaaatac taaaaaatat
    11941 tttaaataaa gaacttaaaa ttgtgataaa attaatttat agatattaaa aaaagtataa
    12001 tatatggttt ttaagaggtg ctaagatgaa aaattttaga gatttaggtg ggaacaaaac
    12061 tgaggatggc agaactgtaa agaagggatt attttatagg tctgcaaaat tatcaaatct
    12121 aagtgaaaat gatataaaaa ttttaagaga gttaaatatt aaatatatat ttgattatag
    12181 aagtgatgaa gaagctcgta aacatccatc aacaataata tcaaatataa aaaatataag
    12241 aataccagca atgagagagc tagaagaatc aggaggaagt tttggttcta ttgaagatat
    12301 gattgatgga ttgtttgaaa aagatggtgc atttaatatg ttaaataata gttactataa
    12361 tttgcctata aataatccgt catataaaaa attagtagaa cttataagag actattctaa
    12421 tttaccaata ttaaatcatt gtacagctgg taaggataga actggtgtgg ggagtgctat
    12481 aattcttatg attttagggg tatcgagaga gaatataatg aaagattatc taaaaagtaa
    12541 tgattttgca gataaagaga tagaaagatt tatagaatat aagcctaaat ttaaggacat
    12601 tccaaaagaa aatttgaaat atatatttgg tgtaaatgaa gagtatatga aaactgcatt
    12661 tagaagaatt gatgaagaat acattagtgt agaagcatat ttatatggag aatttaattt
    12721 aaataaagaa gaaataagaa aattaagaaa tcaatatcta gagtaaaagg tgagtttatg
    12781 agtttaaata taacagcaag acagataaat ataataaaaa tattgttaaa ttcaaaagat
    12841 acaattagtg gtattgcctt aagtcaagag ataggatgtt cttcaaaaac tatacaaaat
    12901 gaaataaagg atattaataa acaacttaga aatggtaaaa ttttgtcaat aagaggagtt
    12961 ggttataaac tagaaggaag ttttgatgag gtaaatctaa atagcaatct ctatgatgat
    13021 gtagatagag tagaatatat aatcagaaaa atattaacct taagtaatga agaaaaaaat
    13081 actataaaac ttgaagattt agccgattct atgtatgtaa gtgtttcaac agtaaaaaat
    13141 gatttaaaag aagtaaaaag aatgttggaa aagtattcaa tatcagttaa atctaagcat
    13201 aaacaaggta tatgtgtttt ggaaagtgaa gataagatat taaattgtat agttgactta
    13261 agcaataaga gagataatca attgagttta aatgattttt taaatgatga tataaaggaa
    13321 agaaaactat taataaaaaa attattatta gacatattaa atgaaaatgg attagtatta
    13381 actgatatag aatttaagag tgtgttaaat aatatattag ttatgttaag cagacataag
    13441 tataatgaaa aagaatttat agagaattat atagaaaatt ataggagcaa aagaagctca
    13501 attttagaaa atgaacaaaa caaagaaatt ataataaatt caataaaaac attttgtaaa
    13561 aatttaaaat tagctacatc aatagatata agtaatgata aggtatttga agagttttta
    13621 tataagcata ttagtagttt atgtaaaaaa ataaagttag gtataaatca atcacctata
    13681 atggctcaag atataaaaat aaaatatcca tttgcttttg aactagctaa tatagctaaa
    13741 aagacaatag aaaaagattt gaagatagaa attaatgaag atgaagttgc aaacatagct
    13801 ctacatatag gaggagcagt tgaaagagca tcttataata ataaagataa ggttttcaaa
    13861 actataatag tttgtacttc aggcataggc acatcaatgt taataaaagc taaattagag
    13921 aatatattta aggaaaaatt agaaatacta aagattattc cttcatactt agttgaatat
    13981 ataaaagtta tagatgttga ttttgtaata tctactgttc ctattgaagt aggagatgtg
    14041 ccagtaataa atatatctcc aattttaagt gagaaagaaa ttagattaat tgaaaaatat
    14101 atagaaacag gaaatatata cctagattta gatataaaaa gcctgtttga tagtgaattg
    14161 ttttttactg atttggtgtt taaaaggaaa gaagaagtta tagattttat ggcaagtaaa
    14221 cttgtacaaa agggctatat agatgaggat atgaaaaaat cttactttga gagagaaaaa
    14281 atagcaacaa cagaaatagg gaatatggta actataccac atggagctat tggaaaaatt
    14341 tttgataata aaatagcaat tggtatatta aagaagccta tttcttggga aatatcagat
    14401 gttcgtttaa taataatgtt agcccttgat aaagataaaa tattagatta tgaagtaata
    14461 ttttcaaaaa tatataaaag agtagattct atagcaaaag ttataagtat atgtgaaaat
    14521 agtagttttg aaaaatttgt aaatttattt aattagatgg gtagtattgt atatacacct
    14581 gtaaatttgg ataatataaa actaaataag ataaaaacga taaatttctc ttttaattaa
    14641 gagaaatttt ttgactttca aggatattaa tataaagtta agatataaac ataaagcaaa
    14701 taaataaaaa agaggtggca atatgattta tacagtgaca ttaaatccat caatagatta
    14761 tatagttaag ctagatgagc taaagacagg aagtacaaat cgtgtaaaag aagaatacgt
    14821 ttatccagga ggaaaaggta taaatgtatc tcgcatactt aaggaacttg gaaatgataa
    14881 tacatcttta ggttttataa gtggttttac tggagaatat ataataagaa cattggaaga
    14941 aaaagaattg aagacagatt ttataaagat aaaaagtggt ttttcaagaa taaatgtaaa
    15001 aataaaggcg ttaaaggaaa ctgagattaa tggacaaggt ccaaatatag atgatgaaga
    15061 cattgatact ttatataaaa aacttgataa gctaaatcaa gatgatattt taatattggc
    15121 aggaagtata ccatctactt tagatgaaaa actatacgaa aatataatgg ctagattaga
    15181 aaaaaagaat ataaaagttg tagtagatgc aactaaaaat cttcttctta atgttttaaa
    15241 atataaacct tttctaatta agcctaataa cgacgaatta gaagaactat ttggtgttaa
    15301 attaaattca attgaagaca tggtaaaata tgctagaagg ttaaaggaaa tgggagctat
    15361 aaatgtcttg gtatcaatgg gaaaagatgg agcattgttg attacagagg aagatgaagt
    15421 gcttataagt gatgttccaa aaggaaaagt aaaaaattct gtaggagcag gtgattcaat
    15481 ggtagcagga tttatatctg ggtatttgaa tactgggaaa tatgattatg ctcttaaatt
    15541 gggagcagct tcaggtagtg caactgcttt ttcatatgac ttagccaaaa gagaatacat
    15601 tgataaattg gtaaatgaga tatctgtaaa gcagttttaa aatattaaaa aatatataga
    15661 aaggtaatta atatttatga aaattataga tttattagat gaaaagtcaa ttaagttaaa
    15721 tttaaattca aaaaccaaat cagaagcaat agaagaatta gtagatttgg tcgcaaatag
    15781 tggaaattta aatgataaag aaaactacaa aaaagcaatt ttggctcgtg aagaaatgag
    15841 tacaacagga attggagaag gtgtagctat acctcatgct aaaaattctt cagttactaa
    15901 agcttgtata gcagcagcag tgtcaaaaga aggtatagat tatgaatcgt ttgacggaag
    15961 tttatcaaat ctatttttta tgatagctgc accagacgga gctaacaata ctcatcttga
    16021 agtattatca agattatcta caatacttat ggatgaagac tttagaaata aacttataaa
    16081 ttcaagctct gaaaaagaat ttaaggaaat aatagataaa aaggaaagag aaaaattcag
    16141 tgaagaatat caagatgaga aagttactga aaaaaatata atagatacta aagagaatga
    16201 tgctaataag tatcctaagg ttttagcagt aacagcatgt ccaactggaa tagcacatac
    16261 atttatggca gctgaaagtt taaataaaat ggcagaaaac aaaggtgtat caataaaagt
    16321 agaaactaat ggttcagcag gtgttaaaaa taagcttaca aaagatgaaa tagaaaatgc
    16381 aacatgtata atagtggcgg cagataaaaa tgttgagatg gctagattta atggtaaaaa
    16441 agtcataaaa actaaggtgg ctgatggtat acacaaagcg gaagaactta tagacaaggc
    16501 tgtaaatgga gatgctccaa tttaccatgg aggagatgga agtcacaacg aatcaaatga
    16561 agaaagtgaa agtggattta gaaaagtata caaacactta atgaatggtg tatctaatat
    16621 gttaccattt gttattggtg gagggatact tatagcgata gcctttttac ttgatgatta
    16681 tactattaac ccttcaaact ttggttcaaa tacacctata gcagcatttt ttaaaggaat
    16741 aggagataaa gcttttggat ttatgttacc tgtacttgct ggatatatag catactctat
    16801 atctgataga ccagcgtttg ttgtaggatt tgtaggaggt gctctagcag gtgatggtgg
    16861 ttcaggtttt ttaggtgcac tacttgcagg ttttatagca ggttatttag tagaagggct
    16921 taagaaaata tttagtgtac taccagcttc attggaagga ataaaaccag tattgttata
    16981 ccctttactt ggaacacttt tgatgggaat tataatgaca tttttagtaa tacctccagt
    17041 tacagcaatt aataatgcaa tggttggatt tttaaatggt ttaggtggaa cttcaaaaat
    17101 agttttagga ttagtattag gtggaatgat ggctgtggat atgggtggac ctgtaaataa
    17161 agcagcatat gtatttggag ttgcatcact tgaatcaggg cagtttgaaa taatggcagc
    17221 agtaatggct ggtggtatgg ttccgccact tgctatagct cttgcaacta cattttttaa
    17281 gaatagattt actaaagaag aaagagattc tggtaaagtt aattatgtaa tgggattatc
    17341 atttgttact gaaggagcta taccttttgc agctggagac ccacttcatg ttataccagc
    17401 ttgtgttgga ggttcagcag tggcaggagc acttagtatg ttgttcaacg cagctcttcg
    17461 tgcacctcat ggaggagtat ttgttatacc agtggttacc catccatttg catatatatt
    17521 agctatagct gtaggtgctt tggttggaat gatgctttta gcattactta aaaaaccatt
    17581 aaatcaagaa gtatagcaat taaaggatat tcaagaactt aaaatatgat gttttataaa
    17641 aattaatata ttgctgaatt attttaaaat aaggatttta tgaggctgtt atataacgat
    17701 ttgtaattgt tatcttgaca gtcttttatt tttatataag taaaataagt gtgttttgct
    17761 ttatttgtat ttaaattgtt tttaagttag aatataaaga aaattatata ttattttaat
    17821 attcactcga actagaaaat aatatttgtt gtatttttt
//
LOCUS       DAGSRM010000081        17850 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.081, whole genome shotgun sequence.
ACCESSION   DAGSRM010000081 DAGSRM010000000
VERSION     DAGSRM010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17850
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.081"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            226..2289
                     /locus_tag="MJE33_002805"
     CDS             226..2289
                     /locus_tag="MJE33_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBY9648732.1"
                     /translation="MTKIILSNYRCFGEEPTTIEINNLTGFIGHNSSGKTALLSALMK
                     LFGEKSSDRIIERSDFHIPESIDPDSISKNELYIEAQFEFPELERGKDSISIPVFFKN
                     MVVSEPGGKPYLRIRLEANWEKSSNPEGSIDSNVYYITSTTEEINPEDKRNIKRYELD
                     QIKMIYVPAIRRPDEQLKNVSGTIISRLLNGISWSEQTKARIKTKASETEEIFFEEKG
                     VESLKKSLETQWNSYHKDIRYSNPKINFNSTDLETILKKVEVTFSPTGTHRDYRVEEL
                     GDGLRSLFYLSLVNTFLEIEEEILKSINDGKEEDIFTLIPPVLTIVAVEEPENHISPH
                     LLGRVVEKLNFISTKENSQTILTSHSPAIVKRIEPTDIRYFRICKEYECTKVKSIILP
                     NEEKEAEQFKFVKESVLAYPELYFAKLVVLGEGDSEEIIIKQMIELKAESVDSSEISI
                     VPLGGRHVNHFWRLLNDLEIPFITLLDLDRERDGGGWGRIKYAIQQLINIGHDKNVLL
                     YTCEGISQDNEFKEMHTWDVTDTENMAKWIEMLKGYDVYFSAPLDIDFAMLQSFKDKY
                     ISALTSAEGPRIKDHGKIQDIVINEDAEDESLKAAYKDRIDSDIKSTLKQEGGDGSTY
                     TEGEKELMIWYSYFFLGRGKPTTHLSLFSNNTDIDFESFPYCLKEFVNSIIEKMIDDS
                     GGGRK"
     gene            2286..4151
                     /locus_tag="MJE33_002806"
     CDS             2286..4151
                     /locus_tag="MJE33_002806"
                     /inference="COORDINATES: protein
                     motif:HMM:NF024642.1,HMM:NF024754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBY9648733.1"
                     /translation="MKRNWVSKEDWSPAAGFRLEVEAMNTVINDKNTLVVAGPGAGKT
                     ELLAQRACFLLETNTCRYPKKILAISFKKDAAENLKERLELRCGKELASRFESKTYDA
                     FAKEIVDRFRNSLDEAYRPLNNYNIAENRDVRRAFEIAGLNFRGNQTEFNKVYGNKLS
                     INKLPLHTDFVDKIFIDAWNVLLKGNKDTNFVSSLTFGMISRLAEYLIRTNRYIKKAI
                     EMTYSHVFLDEFQDTKSIQYDLVKACFFKTETIITAVGDGKQRIMLWAGARKSIFEDF
                     QNDFLSQKRTLIMNHRSAPRLVEIQKAMYNSLNEEAIDLKTNEKWNYEDGEVYLRLFE
                     DYIKEADILSEEIVELVETGIEINEICILVKQSVDIYSKEIINKLSERNIKARNEAMY
                     QEILKEDIIKTLVSLFRLSLSDRNPDDWDYIYDILGELYGIDEGYNPKILNDFITKVE
                     LMINDLKVNLNITDEKQFSDLVDSKVEYISYEKFASKFQKYKSKDYFDDLVDKFKKLV
                     WIEYNETKDWIKAIDNFEGKNSIPIMTIHKSKGLEYDTIFFVGLEDSAFWNFKNQPDE
                     DRCAFFVALSRAKRRIDFTFSLDRPAGFSNSQKHYCINEFYELFDNTEIVKKIEY"
     gene            4275..5089
                     /locus_tag="MJE33_002807"
                     /pseudo
     CDS             4275..5089
                     /locus_tag="MJE33_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728762.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5114..5602
                     /locus_tag="MJE33_002808"
     CDS             5114..5602
                     /locus_tag="MJE33_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBY9648734.1"
                     /translation="MYEKDYKQYIKLDIPIDEAKIKNRFEIRLKNERAYHAAVDLLAN
                     RDEEKTAFSIINRYIRFVDKDDEKIRSEWKTNDQWSYFIGEGRAKLKLTTNPEPYNIQ
                     KTLNWLSRQVAPTLKTIKRLDSLNNSTLIEGLIRYARLTDKHKKIIEQNSVNVEDIIY
                     DE"
     gene            5715..5876
                     /locus_tag="MJE33_002809"
     CDS             5715..5876
                     /locus_tag="MJE33_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648735.1"
                     /translation="MAIAIIGVYLGFKREFSKLIGFLVVALIAVGLVFNTAGVKDILL
                     ELFNKIIGV"
     gene            6116..6343
                     /locus_tag="MJE33_002810"
     CDS             6116..6343
                     /locus_tag="MJE33_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY9648736.1"
                     /translation="MDIVKVFANNVRKYRNKKGLSQEAFAEKAGLHRTYICAIERKKR
                     SIALDNVQKIADALEIETYLLFIEGEEKECL"
     gene            6340..9279
                     /locus_tag="MJE33_002811"
     CDS             6340..9279
                     /locus_tag="MJE33_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBY9648737.1"
                     /translation="MKKLPIEKLIENLGFSGSKNLVNIDMLTKLDISKHDVKTLKVIN
                     PYAIYLVNSKPFILFIESSFEEKKLQQISKLIWNAQIPIAFICDSNSVKIFNGKALDL
                     KSYLIEEIYNQSIEDFSEESEFSYLKISDPLFWDQYTKQYTKTHLNDYLLDNITSLTN
                     KLKKKYKVKFATKLVLRLIFIRYLIDRGVDIAYKNFNHDIKASQKELLKITKNKVELY
                     ELFKYLKEKFNGNLFELNDEINNTCLTDEVFILLSDFLSGKISIDNGQMSLFSMYDFN
                     IIPVELISNIYEILLGKEIRNKDNAFYTPNYLAEYILDKTTLEFLKNNRQYKILDPSC
                     GSGVFLVNSFRRIIDMNLGDKLFCEDDKLLKELLLSNIYGIDINEDAIDVTIFSLYLT
                     ILDYKDPKILSEFKLPNLKGCNLIASDFFDDNKLESLKKINFDFIIGNPPWGSVSDGL
                     HLDYCNKNGYNDMQQNKEISRSFIFRAKDFSSRNTVCSFVVHSKILYTQKKPSKKFRE
                     FLLKKSQIFSVVEMSSVRKLVFKNADAPAAVISFKYNESEENLNNRIIYTSLKPNVFF
                     KLFNIIVIEKYDVKYISQKLLLENDWAWKTIVYGFSKDIDIIMNIKKKYQTVNEIILK
                     NSFVHGTGIQINGNESHDATHLKGKWYVDSKKGIDSFYVDINKGKELTQDKMRRPKAN
                     QQEIFIPPYVLLKKGVDTKTYRYRAAFSNDAFVYSDAVTGICGKKDNLTTLYCLTGML
                     NSSFYSYLNIMLGTSIGIEREQGFSTEIFTYPAIESDEIANLVVKIQEATKLEKTTMF
                     LNEKNSCKYIKQLDKLILKSFNLNDDKFIDYAINIQIPELTNSKSTNIYRKITKEDLI
                     NYSECFKEQFSSIYRRVGKSISISVYPKILNRFTVFELEVTDEKTDREIMVKAEADNN
                     MELFTKIIRYDYNDVFHQIRDIIHFGENSFYIIKPNCYKYWHPAIAEMDLADVLDQIL
                     SDTGGEE"
     gene            9279..9953
                     /locus_tag="MJE33_002812"
     CDS             9279..9953
                     /locus_tag="MJE33_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648738.1"
                     /translation="MGGFKNSSLNFYLKQGLYEIIIDGLIDCCLLMSKSCIEQSRKIL
                     NHEEKIRTHLVENYLENDIERRKTSLSKQHLRFEVEAQENYDNKNESYEGRVDIKVIS
                     KDTLADRRAYYIIECKRIDGKETLNKKYITEGVQRFITEKYSSHYGKNIMLGFIVKDI
                     NIQSNIQKINSLNQNILYTYIDTDMLTVRSDSENNYAVCESKYKVKSKLLLLNHIFYN
                     FSSIIR"
     gene            10114..10530
                     /locus_tag="MJE33_002813"
                     /pseudo
     CDS             10114..10530
                     /locus_tag="MJE33_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455547.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            10490..12937
                     /locus_tag="MJE33_002814"
                     /pseudo
     CDS             10490..12937
                     /locus_tag="MJE33_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            13077..13499
                     /locus_tag="MJE33_002815"
     CDS             13077..13499
                     /locus_tag="MJE33_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648739.1"
                     /translation="MRLEDIIDKESYEEKYDDLTVEIKKLSKEKKTLKASLINEKSIK
                     ARLREFRKILERNELLEKFDRYVFASIVDKVIVGVINEDGIVDPYKITFVYKTSFDDK
                     QDGHKFINKSPLETYSKDNNDNKKKCSYTNNETLRDCS"
     gene            14037..15071
                     /locus_tag="MJE33_002816"
     CDS             14037..15071
                     /locus_tag="MJE33_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HBY9648740.1"
                     /translation="MFKKKLIAVAMSITMISVGSFSYAHSGRTDSSGGHRDNKNKSGL
                     GSYHYHCGGYPAHLHNNGGCPYTGGGSSSGTTTSVNNEEKQKRSVGEKGYNQGYEDGY
                     KGNYSSSNYSGDYSDTYESKYSEGYEKGKAKLEEEEKVAKETGYNLGLTGAKSNNTYE
                     KEVLKNAYDTGYSTGYNEYKTKKIEEYKAKGIEDSNKDKEKMTFEENIDSEFIDAYNN
                     AYDERQEQLKNDYTTQGFESAIKGERFDTSTIGNVKYANWFKEGYDNGKVKLPKAKES
                     VYNQGYNEEDFSIPDEMKSIETKLKGVYDEGLEKREEEKSRNATYGVGAGTAAVVAGV
                     VYKKKKRKKI"
     gene            15158..15361
                     /locus_tag="MJE33_002817"
     CDS             15158..15361
                     /locus_tag="MJE33_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HBY9648741.1"
                     /translation="MGFNFRKSINLGGGLKLNLGKKSVGISAGVKGARVSVNSKGRKS
                     ATLSIPGTGISYTKTSTKKRKPK"
     gene            15532..16692
                     /locus_tag="MJE33_002818"
     CDS             15532..16692
                     /locus_tag="MJE33_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp27"
                     /protein_id="HBY9648742.1"
                     /translation="MKLSKILLAIGMSVSLLIASCPTSNALSSINSIKGTNKYETAGL
                     IADKQNYDIAILINSENGLSDGLSASGLSGAKNAPILLTKKDTIPSETLKRLEKVKEV
                     YLIGGKNSIGASVQKILSDKKISINRISGTDRIDTSHKVAKEIGRVSTVKKVILTNAF
                     KGEPDAMSVASIAVRDKSPIVLTDGNTTSFATDGLETYAIGGKVSMSDKLINETKATR
                     IGGVDRYDTNKQIINKFYNGSKEFYIASGTDLVYSLVGSTIAQKTPIVLVGNGSNKSI
                     LKNASKITAIGNIDDVVIKQCLNITNNVGDSNTGIVKDTNSNNTNKPSNEPSSNNPGL
                     TDKSTVYVTPNGKSYHLTKNCSTLKRSKTINKVTLKQAKSQGKIDPCNVCVK"
     gene            16951..17091
                     /locus_tag="MJE33_002819"
     CDS             16951..17091
                     /locus_tag="MJE33_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648743.1"
                     /translation="MENLMMSIITGVIASYIYDKLKSHSERPTKSGWELNIKFQKSKS
                     SK"
BASE COUNT         7302 a         1721 c         3047 g         5780 t
ORIGIN      
        1 tttataaatt taatatctat tttttatgtg aaaatatatt taatctaaag aatgatattt
       61 cataaaaaaa attaaatata tttttaaaat atttacaata gagttatttt ttattcgtat
      121 aataatagaa atattttagc ataagatgta taataaagga aaaaatacag aattaaaaaa
      181 cataagatag ataagtggac ttaggggaga gaaattatta tgcaattgac aaaaataatt
      241 cttagtaact ataggtgttt tggagaagaa cctacaacta ttgaaattaa taatttaact
      301 ggatttatag gtcataatag cagtggtaaa actgctttat taagtgcact tatgaaactt
      361 tttggagaaa agtctagtga tagaattatt gaaagatcag atttccatat acctgaaagt
      421 attgatccag atagtatttc taagaatgaa ttatatattg aagcacaatt tgagtttcct
      481 gaattggaga gaggtaaaga cagcatatct ataccagtat tttttaaaaa tatggttgtt
      541 agtgaaccag gtgggaaacc ttatttaaga attagacttg aagcaaattg ggagaaaagc
      601 tcaaatcctg aaggaagtat tgattcaaat gtatactata taacatctac aacagaggag
      661 ataaatcctg aagataagcg taatatcaaa agatatgagc ttgatcaaat aaagatgatt
      721 tatgtacctg ccattaggag accagatgaa cagttaaaaa atgtctcagg aacaataatt
      781 tcaagattat taaatggtat aagttggtca gaacaaacaa aggcaagaat taaaacgaaa
      841 gcaagtgaaa cggaggaaat tttctttgaa gaaaaagggg ttgaatcatt aaagaaatca
      901 ctcgaaactc aatggaatag ttatcataag gatattagat attctaatcc aaagataaac
      961 ttcaatagta ctgatttaga gacaatttta aaaaaagttg aagtaacttt ttcacctaca
     1021 gggacacatc gagattatcg ggttgaagag cttggagatg ggttacgttc tttattttat
     1081 ctttcacttg taaatacatt tttagaaatt gaagaagaaa ttctaaaatc tattaatgat
     1141 ggtaaagaag aagatatatt tacattaata ccgcctgtat taacaattgt tgcggttgaa
     1201 gaaccagaaa atcatatttc acctcatctt ttaggaaggg tagttgaaaa attaaatttt
     1261 atttctacaa aagaaaattc acagacaata ttgacatcac attcaccagc aatagtaaaa
     1321 agaattgaac caacagacat aaggtatttt aggatttgta aggaatatga atgtacaaaa
     1381 gtgaagtcaa ttatattacc aaatgaagaa aaggaagcag aacaatttaa atttgtaaag
     1441 gaatcagtat tagcatatcc tgaactttat tttgcaaaac ttgttgtatt gggcgaaggt
     1501 gatagtgaag agatcattat taagcagatg attgaattaa aagcagaatc tgtagattca
     1561 tcagaaattt caatagtacc attaggtggt aggcatgtta atcatttttg gcgtttatta
     1621 aatgatttag aaattccatt tattactttg ttagatttag atagagaacg agatggaggt
     1681 ggttggggac gtataaagta tgcaatacaa caattaataa atattggtca tgataagaat
     1741 gtattacttt atacatgtga aggaatttca caagataatg aatttaaaga gatgcatact
     1801 tgggatgtaa ctgatactga aaatatggct aaatggattg aaatgctaaa aggttatgat
     1861 gtttattttt cagctccgtt agatatagat tttgcaatgt tacaaagttt taaagataaa
     1921 tatatttcag cattaacaag tgcggaaggt cctaggataa aagatcatgg aaaaatacaa
     1981 gatattgtga tcaatgaaga tgctgaagat gaatcattga aagctgctta taaagataga
     2041 atagatagtg atattaaatc aaccttaaaa caagaaggtg gtgatggatc tacttatact
     2101 gagggagaga aagaactaat gatatggtat tcatatttct ttttaggaag aggtaaacca
     2161 actactcatt tatctttatt ttctaataat actgatatag attttgaaag ttttccatac
     2221 tgtctaaagg agtttgtaaa tagtattatt gaaaagatga tagatgattc agggggtgga
     2281 agaaaatgaa gaggaattgg gttagtaaag aagattggag tccagcggct ggattcagac
     2341 tagaagttga agcgatgaat acagttatta atgataagaa tacacttgtt gtagctggtc
     2401 ctggtgctgg aaaaacagag ttattagctc agagagcctg ctttttacta gagactaata
     2461 cctgtagata tcctaaaaaa atccttgcta ttagttttaa aaaggatgct gctgaaaatt
     2521 taaaagagcg tttagaattg agatgtggga aagaattagc ttcaagattt gaatcaaaga
     2581 cctatgatgc ttttgcaaaa gaaatcgtag atagatttag aaatagttta gatgaagcat
     2641 atagaccttt aaataattac aatatagctg aaaatcgtga tgttagaaga gcatttgaga
     2701 tagcaggatt aaatttcaga ggaaatcaaa ctgaatttaa taaagtatat ggaaataaac
     2761 tatcgataaa taaattgcct ttacatacag attttgtaga taagattttt atagatgctt
     2821 ggaatgtact acttaaaggc aacaaagata ctaattttgt aagttcactt acttttggta
     2881 tgatatcacg tttagccgaa tatttgataa gaaccaatag gtatattaag aaagcaattg
     2941 aaatgactta tagtcatgtg tttttagatg agtttcaaga tactaaatct attcaatatg
     3001 atcttgttaa agcttgtttt tttaaaacag aaacaataat tactgctgta ggggatggca
     3061 aacaacgaat tatgttatgg gctggtgcta gaaagtcaat atttgaagat tttcaaaatg
     3121 attttttaag tcaaaaaaga acattaatta tgaatcatcg ttcagcgcca agacttgtag
     3181 agattcaaaa agcaatgtat aacagtttga atgaagaggc aattgattta aaaactaatg
     3241 aaaagtggaa ttatgaagat ggagaagtgt atttaagact ttttgaggat tatataaaag
     3301 aagctgatat acttagtgag gaaatagtcg aattggtgga aacaggtata gaaattaatg
     3361 aaatatgtat attagtaaaa cagagcgtag atatttattc aaaagagatt ataaataaac
     3421 tttctgaacg taatattaag gcgcgtaatg aagcaatgta tcaggagata ttaaaagaag
     3481 atataataaa gacactggtt tcgcttttta ggttgtcgtt aagtgataga aatcccgatg
     3541 attgggatta tatttacgat attctaggtg aattatatgg aatagacgaa gggtataacc
     3601 ctaaaatatt aaatgatttc attaccaaag tagaattaat gattaatgat ctcaaggtaa
     3661 atttaaatat tactgatgaa aaacaatttt cagatttagt tgattctaag gttgaatata
     3721 tttcatatga aaaatttgca tcaaagtttc aaaaatataa atctaaagat tattttgatg
     3781 atttggtaga taaatttaaa aagctggtat ggattgaata taatgaaact aaagattgga
     3841 ttaaagcgat tgataacttt gaaggtaaaa attcaattcc aataatgaca attcataaaa
     3901 gtaaaggact tgaatatgat actatctttt ttgttgggtt agaagattca gcattttgga
     3961 attttaaaaa tcaaccagat gaagatagat gtgcattttt tgttgcatta tcaagagcta
     4021 aaagaagaat tgattttaca tttagcttag atagaccagc aggattttcg aattcacaga
     4081 agcattattg tattaatgaa ttctacgaat tgtttgataa tactgagatt gtaaaaaaga
     4141 tagagtattg attaagatga taaaaacttt agtatattat attttaagat attagttata
     4201 taagtagaca ttgaatttat gtatttgatg tctatttttt atataaaaat atattaaaga
     4261 aaggatgaat ttgcatgaag aaaaaaacaa aaatatctaa aaagattgta agttttatta
     4321 tggtttttat aactatagtt actactatac ttacatcatc agtttcagct tatgctagcg
     4381 agttggatat gagtgaacag acaggcttta attatttagg tattagtcca attacaggaa
     4441 aaaaattaat cataacattt atcgtatgaa gatggataat aaaattgtat tttgtgtaga
     4501 gccaggaatt aaaacttcaa gtggaggagg atatatatcg gaggagttag gagataaatt
     4561 tatatctaca acacttccaa attatcataa aagaaaagct gaaatacttg agaaggtaaa
     4621 tagacataat atattacctt catggaataa tgaaaaagtt gcagttaaaa gtggagaaac
     4681 aattgagtta gaagataaga atggtattat tagagaaatg caattagaat caaataatac
     4741 tggaacagat atattacttg aagaaaatac tttgaaaatt acagcaaata aaaattctaa
     4801 ttcaggagtt attagttata taaagattcc taaaaatgag gttggaatat caatagttta
     4861 tcgtaagcat aactatcaaa gcttatcaga atttcatatg gaaagttcga tgagtgcaaa
     4921 tttaaatata aatgtaatca agttaggaat tgttaaagta aaaaagattg atgaaggcac
     4981 aggaaaggaa ttagcaaatg caaaacttag atttgagtat gatggtgata gtaaagaaat
     5041 tataacgggt tctaatgaaa ttgctagtat agaagatatt attggttgat taaaaagtga
     5101 agtatacttt tgtatctatg aaaaagacta taagcaatat ataaaattag atatacctat
     5161 tgatgaagca aaaataaaaa atagatttga aataaggtta aaaaatgaaa gagcatatca
     5221 tgcagcagtt gatttgttag caaatagaga tgaagaaaag acagcatttt caattattaa
     5281 tcgttatata cgttttgttg ataaagatga tgaaaaaatt agaagtgaat ggaaaactaa
     5341 tgatcagtgg agttatttta ttggtgaagg tagagcgaag ttaaaactaa caacaaatcc
     5401 agaaccatat aatattcaaa aaactttaaa ttggttatca agacaagttg caccaacgtt
     5461 aaaaacaatt aaaaggttag atagtttgaa caactcaaca ttaattgagg gcttaataag
     5521 gtatgcaaga ctaacagata aacataaaaa aataattgaa caaaattcag taaatgttga
     5581 agacattatt tatgatgaat aaaaaaatgt aattagtatt gcagatttta atataaaaga
     5641 agggagatat acgaatgaat tttggtcaaa atttatataa ttagttttta tcaaatgcac
     5701 aatcattggt attaatggct atagctataa ttggtgtata tttaggattt aagagagagt
     5761 tttcaaaact tataggattt ttagtagttg cattaattgc agtagggctt gtgtttaata
     5821 cagcaggagt taaagatata ttattggagc tatttaataa aataataggt gtgtaaggat
     5881 taaaagagaa tagaaaatta tgagtatatg atgaagggtt ctaataagtt ttattaaaat
     5941 atatattgaa agacaagtag agctatttcg agttattaca tgtaatgcta atatatataa
     6001 ctgcattttg gaaattaaat acataattag aaatagcaaa ttttataggt gacttattaa
     6061 tatgtttact tattgtatac tatttgatat atttaatatg aggtgattct tgaaaatgga
     6121 catagtgaaa gtttttgcta acaatgtgag aaaatataga aataaaaaag gtctatcaca
     6181 agaagctttt gctgaaaaag ctggattgca tagaacttat atttgtgcta ttgaaagaaa
     6241 aaagagaagt attgctttag ataatgtgca gaaaattgct gatgcgcttg agattgagac
     6301 ttacttacta tttatagaag gtgaggagaa agaatgtcta tgaaaaaatt accaatagaa
     6361 aagcttatag aaaatttagg tttttcaggg tcaaagaatc ttgtgaatat agatatgctt
     6421 acaaaattag atatatctaa acatgatgta aaaactttaa aagttataaa tccttatgca
     6481 atttatttag taaatagtaa gccatttata ttatttatag agtcttcttt tgaagaaaaa
     6541 aaattacaac aaattagtaa gcttatatgg aatgctcaaa taccaattgc ttttatatgc
     6601 gattctaatt ctgttaaaat atttaatgga aaagctttgg atttaaagtc atatttaatt
     6661 gaggaaatat ataatcaatc tattgaggat ttttcggaag aatctgagtt ttcttatttg
     6721 aaaatatctg atccattatt ttgggatcaa tacactaaac aatataccaa aacgcattta
     6781 aatgattatt tgctagataa tattacttca ttgacaaata aattaaagaa aaaatataag
     6841 gtgaaatttg caacgaagct tgttctaaga ttaatattta ttagatattt aatcgataga
     6901 ggtgtcgata tagcttataa aaattttaat catgatatta aagcttcaca aaaagaactg
     6961 ctaaagatta ctaaaaacaa agttgaatta tatgaactgt ttaaatactt aaaagaaaag
     7021 tttaatggta atttatttga gttaaatgac gaaattaata atacttgttt aacagatgaa
     7081 gtgtttattt tactatctga ttttttatca ggtaaaataa gtattgacaa tggacaaatg
     7141 tctttatttt caatgtatga ctttaatatc ataccagttg aactaattag taatatatac
     7201 gaaattcttt taggaaaaga aattcgtaat aaagataacg cattctatac tccgaattac
     7261 ttagcagaat atattttaga taaaacaaca cttgaatttc tgaaaaataa taggcaatac
     7321 aaaattctag atccttcttg tggatcagga gtatttttag taaatagttt tagacgtatt
     7381 attgatatga atttgggtga taaacttttt tgtgaagatg ataaactttt aaaagaactt
     7441 ttattatcta atatttatgg aattgatatc aatgaagatg ctatcgatgt tactattttc
     7501 tcgttatatc taactatttt agattacaaa gaccctaaga tattatcaga atttaaactg
     7561 cctaatttaa aagggtgtaa tttaatagcg agtgattttt ttgatgataa taaattggag
     7621 agtttaaaga aaattaactt tgattttatc attggaaatc cgccttgggg aagtgtctca
     7681 gatggattac atttagatta ttgcaataaa aatggctata atgatatgca acaaaacaaa
     7741 gaaatcagta gaagttttat ttttagagca aaagatttta gctctagaaa tacagtatgt
     7801 tcttttgtag tgcattctaa aatactatat actcaaaaaa agccatcaaa aaaattccgt
     7861 gaatttttgc ttaaaaaaag tcaaatattt agtgtagttg aaatgtcatc tgtaagaaaa
     7921 ttagtgttta aaaatgcaga cgctcctgca gcagttatat catttaagta taacgaaagt
     7981 gaagaaaatt taaataatag aatcatctat acatctttaa aaccaaatgt attttttaaa
     8041 ttatttaaca ttattgtaat tgaaaaatat gatgttaaat atatttcaca aaaactatta
     8101 ttggagaatg attgggcttg gaaaactatt gtgtatggat tcagtaaaga tatagatata
     8161 attatgaata tcaaaaaaaa atatcaaact gtaaatgaaa ttattttgaa aaacagtttt
     8221 gttcatggaa cagggataca aatcaatggt aatgaaagcc atgatgcgac acatttaaaa
     8281 ggaaagtggt atgttgattc aaagaaagga atagattcat tctatgttga tataaataaa
     8341 gggaaagaac ttactcaaga taaaatgaga agacctaagg ctaatcagca agaaatattt
     8401 attcctccat atgtattatt aaaaaaggga gttgacacga aaacatatag atatagagca
     8461 gcattttcaa atgatgcatt tgtatattca gatgcagtta caggaatatg tggaaaaaaa
     8521 gataatttaa ctacacttta ttgcttgact ggtatgctta actcttcttt ttactcatac
     8581 ttaaatataa tgctaggtac atcaattgga atagaaagag aacaaggatt ttcaacggaa
     8641 atttttacgt atccagcaat agaaagtgat gaaattgcta atctggtagt aaaaatacaa
     8701 gaagcgacta aattagaaaa aacaactatg ttcttgaatg aaaaaaatag ttgtaagtat
     8761 atcaaacaat tagataagtt aattttgaag tcttttaatt taaatgatga taaatttatt
     8821 gactatgcaa taaatattca aattcctgag cttacaaatt ctaagtcaac taacatatat
     8881 agaaaaataa ctaaggaaga tttaattaat tatagtgaat gtttcaaaga acagttttca
     8941 tcgatatatc gtagggttgg gaaaagtatt tcaatttcgg tatatccaaa aatattaaat
     9001 cgttttacag tatttgaact agaagtaaca gatgaaaaaa cagatagaga gattatggtt
     9061 aaagcagaag ctgataataa tatggagttg tttacgaaaa taattagata tgattataat
     9121 gatgtttttc atcaaattag agatattatt cattttggag aaaattcatt ttatatcata
     9181 aagcctaatt gctataaata ttggcatcct gctatcgcag aaatggatct tgctgatgtt
     9241 ttggatcaga ttttatctga tacagggggt gaagaataat gggaggattt aaaaattctt
     9301 cactaaactt ttatttaaag caagggcttt atgaaataat tatagatgga ttaatagact
     9361 gttgtttact tatgagcaaa tcatgtattg aacaatctag aaaaatatta aatcacgaag
     9421 aaaagataag aacgcattta gttgaaaatt atttagaaaa tgatattgaa agaagaaaaa
     9481 catctttatc taaacaacat ctaagatttg aagtagaagc acaagaaaat tatgacaaca
     9541 aaaatgaatc ttatgagggt agggttgata taaaagttat atctaaagac actttagcgg
     9601 atagaagggc ttattatatc attgaatgta aaaggataga tggtaaggaa actttaaata
     9661 aaaaatatat aaccgaaggt gtacaacgat ttattactga gaaatattct tcgcattatg
     9721 gcaaaaatat aatgcttgga tttatagtaa aagatataaa tatccagagc aatatacaaa
     9781 aaattaattc actgaatcag aacatattat atacatatat agatacagat atgttgacag
     9841 ttagaagtga ttctgaaaat aattatgctg tttgtgaaag taaatataaa gtaaaatcta
     9901 aactactatt gttaaatcat attttttata acttttcgag tataataaga taatcttatg
     9961 aatataaaat attactatgt taaaattata gtttggtttt gataatatta taaaaataag
    10021 tttaagaata aaatataaag gaaaaaaggg gcgtagcact attttttagt gtgacagccc
    10081 ctttaatttt aaaatcggta aggaggagtg tatttgaaaa aaattaagtc atatacaggg
    10141 attttgaatg tggagaaggt tatatatgca ataaatgatt ttcagcttcc atttccagtt
    10201 acttttacgc agatgacatg gtttgtatta acacttttta taattatatt atttggtaag
    10261 ttacctccat tatttatgat agaaggagca tttttaaagt atcttggcat accaattttt
    10321 ataacttagt ttatgtctca aaagactttt gatggaaaga aaccatatgg atttttaaag
    10381 tcaataatac tatattcatt aagacctaaa ataacatacg caggaaaaaa cattaaattt
    10441 aaaaataaaa ttattgatga aaatataact atagtaagga gtgaattata tgttcccgat
    10501 aaaatatata gagaacaatc ttgtatttaa taaggaagga gaatgttttg catattatga
    10561 attaatacca tataattatt cattcttata agctgaacaa aaatttattg tacatgataa
    10621 ttttaggcaa ttaatatcac aaagtagaaa tggtaaaatt catgcacttc aaatagccac
    10681 tgaaagttct ataagagtta cacaagaaag gttaaagaaa aatattacag gaaaattaaa
    10741 agaaattgct tatgaaaaaa tagacgagca aacagaggca cttgtttcta tgattggcga
    10801 taatcaaatt gattatcgtt tttttattgg gtttaagctt ttagttacag agaaagaaat
    10861 gaatataaag agtataaaaa agtcaatatt agctaatcta tcagatttta tatatgatgt
    10921 taataataaa ttgatggatg actttgtcag tataaataat gatgaaatta tgagattttt
    10981 aaaaatggaa aagttattag aaaataaaat ctcaagaaga tttaaagtta gaaggttaga
    11041 taaaaatgat tttggatatt taattgaaca taattatgga aagaaaggca cagtttttga
    11101 agattatcaa taccatttac ctacaaagaa acttaaaaga gaaacattag taaagaaata
    11161 caatcttata aagccaacta gatgtcttat agaagagaag caaagatact taaaaattat
    11221 acatgaggat tcagaaagtt ttgtagctta ttttactatt gaaattatta ttggagaaat
    11281 ggattttcca tctagtgaga tattctatta tcagcaacaa cagtttgcat ttcctattga
    11341 tactagtatg aatgtggaaa ttgtaacaaa taaaaaggct ttaactacag ttagaaataa
    11401 gaaaaaagaa ttaaaagatt tagacaatca tgcttggcag tcagggaatg aaacagtaac
    11461 aaatgtagta gaagcattag aggctgtaaa tgagcttgta aataatttaa atcaaacaaa
    11521 agaatcaatg tataagttaa gctatgtaat tagggtatca gcagatagca ttgatgaatt
    11581 aaaacgccgt tgtgatgaag ttaaagattt ttatgacgat ttaaatataa aattagtacg
    11641 cctaatggga gatatgatag gatttcacaa tgaatttata ccttcaagca aaaggtatat
    11701 aaatgattat attcaatatg taactagtga ttttctagca ggattagggt ttggagcaac
    11761 tcaatagctt ggagagaacg aaggaatata tattggatat agtttagata caggaagaaa
    11821 tgtctatctt caaccatctc ttgcaagtca aggagtaaaa gaatctgtaa ctaatgcgtt
    11881 ggtagcagca ttctttggat ttttaggagg tgggaaatca tttgctaata acttacttgt
    11941 ttactattca gtattgtttg gagggcaagc tgttttggtg gatccaaaag ctgaacgtgg
    12001 caaatggaaa gaaaatttac cagatatata tcatgaagta aatattgtta atttaacaag
    12061 tgatatagaa aataagggaa tgctagatcc ttatgtaatt atgaaagaag taaaagatag
    12121 tggaagtcta gctattgata tattaacttt tcttacagga ataagtagtc atgatggaga
    12181 gcagtttcct atacttagaa aagcaatcat aaatgttact caagacaaat gcagaggact
    12241 tttgaaggta atagatgaat taaggaaaga taattctcaa atatcaaata atattgcaga
    12301 tcatattgaa agttttacag attatgattt tgcacaatta cttttctcag atggaaatat
    12361 taaaggctca attaatctag aaaaacaaat gaatataata caagttgcag atcttgtttt
    12421 accgaataaa gatactttat ttgaagaata tacaacaatg gaaagtcttt cagtagctat
    12481 gttgatagta attagtactt ttgcattaga ttttatccat acagatagaa gtacttttaa
    12541 aatagtagat ttagatgaag cttggacatt tcttaatgta gcacaaggaa aaactttatc
    12601 taataaatta gttagagcag gatgtgctat gaatgcagga gtgtattttg taactcaaaa
    12661 tgcaggtgat gtatcagatg aaaatttaaa aaacaatatt ggattaaaat ttgcattcag
    12721 aagcacagat atcaatgaaa ttaagagaat acttgaattt tttggagttg ataaggaaga
    12781 tgagaataat caaaaaaggt taagagagtt agagaatgga cagtgtttaa tacaagattt
    12841 atatggacga gttggggtaa tacaaataca tctagttttt caagaactcc ttactgcatt
    12901 tgatacaaga ccacctataa gaaaagaggt ggattaatga aatcgaaagt atttagaaat
    12961 ttaagctgga taactatttt aatagtagta cttttactat tttttattta tataaattta
    13021 tagatttaga gaaacaaata gatacattag ataagaaaag aaaaaaactc atagatatga
    13081 gattagaaga tataattgat aaagaatcat atgaagaaaa gtatgatgat ttaacagttg
    13141 aaataaagaa acttagtaaa gagaaaaaaa ctcttaaagc atcattaata aatgaaaaga
    13201 gtataaaagc aagacttaga gaatttagaa agatacttga aagaaatgaa ttgttagaaa
    13261 aatttgatag atatgtattt gcaagtatag tagataaggt tatagttgga gttattaatg
    13321 aagatggtat agttgatcct tataaaataa cttttgttta taaaactagc tttgatgata
    13381 aacaagatgg acataaattt ataaataaga gtccgttaga aacgtattca aaagataaca
    13441 acgataataa aaagaagtgt tcatatacta ataatgagac attgcgagac tgtagttaaa
    13501 cttattagag aataacaagg tttcagactt ttatattgtg tcttaaaagt agatttgcca
    13561 cctatttgcc accataatta tttttgggtg gcaaatttat tctacaatga tttcaaaaat
    13621 atttaccgtc tggtttctca tgttgttagt tacatgtaaa tatgtatcca tagttgttga
    13681 agttttagca tgtccgagta tatgctggat atcttttata ttagtaccat tttctaataa
    13741 catagtatca gggtttttaa aataaagtgt gtattcttct aaagtagtga taattaaaat
    13801 atcaaatatc actatacaaa ggaaagtgca cgctttttat gtttaacaaa aacgaaatat
    13861 taaaaaactt attgaagaat atgatgtcaa aacaactaca gatattcaag atatgctaaa
    13921 agatttattt acaaatacaa ttcatcaaat atttacattg tcgaatttta gcggtttaaa
    13981 cataatatat tgactgatat aataaaatca tagaatattt tagggggaaa tagattatgt
    14041 ttaagaaaaa gttaatagct gttgcaatga gtataactat gattagtgta gggagctttt
    14101 cgtatgctca ttcaggaaga actgattcat caggaggtca tcgtgataat aaaaataaat
    14161 ctggattagg aagttatcac tatcattgtg gtggttatcc agctcattta cataataatg
    14221 gtggttgtcc ttatactgga ggaggaagca gttctggaac aacaacttcg gttaataatg
    14281 aagaaaagca aaagaggtct gttggagaaa aaggatataa tcaagggtat gaagatgggt
    14341 ataaaggaaa ttatagttca tcaaattata gtggagatta tagtgataca tatgaatcaa
    14401 agtactcaga aggatatgaa aaaggtaagg ctaaattaga agaggaagaa aaggtggcta
    14461 aagagactgg atataattta gggcttacag gtgctaaatc taataataca tatgaaaaag
    14521 aagttttaaa aaatgcttat gataccggat attcaactgg atacaatgaa tataagacta
    14581 aaaagataga agaatataaa gctaaaggga tagaggatag caataaagat aaggaaaaaa
    14641 tgacatttga agaaaacata gattctgaat ttatagatgc ttataataat gcttatgacg
    14701 aaagacaaga acaattaaaa aatgattaca ctactcaagg atttgagagt gctatcaaag
    14761 gagaaagatt tgacacatca actataggta atgttaaata tgctaattgg tttaaagaag
    14821 gatatgataa tggtaaggtt aagttaccta aagcaaaaga aagtgtatat aatcaaggtt
    14881 acaatgaaga agattttagt ataccagatg aaatgaagtc tattgaaact aagttaaaag
    14941 gtgtatatga tgaaggttta gaaaaaagag aagaagagaa aagtagaaat gctacttatg
    15001 gggttggagc tggaacagca gctgtagttg ctggagtagt atataagaag aaaaaacgta
    15061 aaaagatata gatgctagat ataccaatga tgacaagtgt ataacaaata taattttaag
    15121 tataaatatt taaaatatag aagggaaggt tatatctatg ggatttaact ttaggaaaag
    15181 cataaatcta ggaggaggac tcaaattaaa tttaggtaag aagagtgtag gtataagtgc
    15241 tggtgttaaa ggtgctagag tgtcagttaa ttcaaaagga agaaaatcag ctacattgag
    15301 tatacctgga actggaatat catatacaaa aactagtaca aagaaacgta aacctaaata
    15361 aatatttgat atactaagaa atagactgtt acaaatagtc tattttttag tatatagatt
    15421 taaaaagtga caaaattttg aaatataaac taatttcata aaggaaaata ttattttatg
    15481 tagaattata atatttaagt aatattataa aatttatagg aggatttcat tatgaaatta
    15541 agcaaaatac tattggcaat agggatgtct gtttcattat tgattgctag ttgtccaaca
    15601 tcaaacgctc ttagttctat taattcaata aaaggtacaa ataagtatga aacagcaggt
    15661 ttaatagcag ataaacaaaa ttatgatatt gcaatactta ttaattctga aaatggatta
    15721 tctgatggat tgtcagcaag tggattgtca ggagctaaaa atgccccaat attgttaact
    15781 aagaaagata caatcccttc agaaacatta aaaagattag aaaaggtaaa agaagtatat
    15841 ctaattggag gaaaaaattc gataggagca tcagttcaga aaatattaag tgataaaaaa
    15901 ataagcataa atagaataag tggaactgat agaatagata caagtcataa agttgcaaaa
    15961 gaaataggaa gagtaagtac ggttaaaaaa gtaatattaa ctaatgcttt taaaggcgaa
    16021 ccagatgcta tgagtgttgc ttctatagca gttagagata aatctccaat agtattaact
    16081 gatggaaata ctacaagttt tgctacagat ggattagaaa cttatgcaat tggtggaaaa
    16141 gtcagtatga gtgataagtt aataaatgaa actaaagcaa caagaatagg tggagtagat
    16201 agatacgata caaataaaca aataataaat aaattttata atggctcaaa agaattttac
    16261 atagcaagtg ggactgattt agtttattct ttagttggtt ctacaattgc acaaaaaaca
    16321 cctatagtgt tagttggaaa tggaagtaat aagagtatat taaaaaatgc aagtaaaata
    16381 actgctattg gaaatattga tgatgttgtt ataaaacaat gtttaaatat aactaataat
    16441 gttggggatt caaatacagg tattgtaaaa gatactaatt caaataatac gaacaaacca
    16501 agtaatgagc ctagctctaa taatcctgga ttaactgata aaagtacagt atatgtaact
    16561 cctaatggaa aatcatatca tctaacaaaa aattgttcta cactaaaaag aagtaaaaca
    16621 ataaataaag taacattaaa acaagctaag tcacaaggta aaatagatcc ttgtaatgtt
    16681 tgtgtaaaat aatgtattag ccaaatatat attaaaaaag acaattaatt aagattgtct
    16741 tttttattga agaaatataa atttatatta tgtattatta aaaaaaaatt attaattata
    16801 aaaaataata ataaagacga taagtattaa aattttatgt ttccaatatt tatgatataa
    16861 ttaatatggc aaggaataaa tatttgaaaa agctatgagt ggtgtttcca tggaagtttc
    16921 ttcactttct tatgagagga ggtgaaaact atggaaaatc taatgatgag tattataact
    16981 ggtgttatag ccagttacat ttacgataaa ttaaagagcc actctgagcg accaactaag
    17041 agtggctggg aacttaatat aaagttccag aaaagtaaat cttcaaaata atttgttgaa
    17101 ggaaacatca ctcaaagtta agtaaaatat gtttccttgc ttttattata tcacaaatta
    17161 aaaatattaa cacaaaattt tgtgaatata tacttattat tgttctaaaa aaatatgtaa
    17221 ataattgatt tgattgcatg gaattattga tgtaatattt aagttaaata tacacaatct
    17281 caattaaaga agataagtac tataaattca tatcaaaaaa atacataaac ttttaagtta
    17341 taattttata aaaagtcttt tagtatcatt atttcaagag gaattatcat atgaaaaaat
    17401 aacttcatta gaaaagtgga taaaaaactg gtaagaagat gactaaagat tacatatact
    17461 attacttaaa ttaaaaattt aatataattt ataatgtata caatttatta taaaaaattt
    17521 aattttaatt ttctattttt gatactataa tatataatct tgataattta attaaaaatc
    17581 tagattttac ataccaaact ggtttcttat ttcgctatct ttacttatga actcatatat
    17641 tcctataaag gttattttag aaaaagttgg ttatttaaac attaatacca caatttctat
    17701 agattgtttg gtgaataatc tataattata ttatggacta aaaaatccaa tgagctaatt
    17761 tattgttttt ttatatttga gagattacaa cttattagtt tgtatatctc tttttttaat
    17821 aaaatcaata atatataaaa ctattggcat
//
LOCUS       DAGSRM010000082        17371 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.082, whole genome shotgun sequence.
ACCESSION   DAGSRM010000082 DAGSRM010000000
VERSION     DAGSRM010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17371
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.082"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            <1..668
                     /locus_tag="MJE33_002820"
     CDS             <1..668
                     /locus_tag="MJE33_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBY9648744.1"
                     /translation="FILIISSIIVYKSLIFKDDIYVVSKKNLPNEKTHVVENEIKGED
                     NNHSKVTKDIDKNFQNNKITIYISGAVNRPGIVTIESDKRLYDAVELLGGTTKEADLN
                     GVNLSVRLEDEQHYIIPKIGEATSVTSNDDSNKPNQKNESRGEPKNKSNKNISNESKV
                     NINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNGIGDKKYENIKDLI
                     CVD"
     gene            760..1806
                     /gene="selD"
                     /locus_tag="MJE33_002821"
     CDS             760..1806
                     /gene="selD"
                     /locus_tag="MJE33_002821"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:808..810,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HBY9648745.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDDPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFDKVKESGALLVGGHTVDDKEPKYGLSVSGIVSPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFEK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKHVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEVVGNIEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            1824..3236
                     /locus_tag="MJE33_002822"
     CDS             1824..3236
                     /locus_tag="MJE33_002822"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HBY9648746.1"
                     /translation="MIVLSKRELFAMLPSVDEVLSDNRIVEIINEYPRSLVLESVREV
                     IDLKRQFILRLKEDASNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLG
                     RSLLSEDIKDELWCAASRYSNLEYDLDNGERGSRYSHLTSTIKRLTGAEDVLVVNNNA
                     AAVLLVLSTMAKGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYK
                     EAITEDTNVLMKVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKY
                     GLSYEPTVLDSIRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDK
                     LTICALEATLRMYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNANINI
                     EDGFSQVGGGSMPLETISTKVISITPEHMNVSSLEKKLRLSEAHIIARVYDNKYVLDV
                     RTIFDDEFDVIVEELRKAFN"
     gene            3350..5233
                     /gene="selB"
                     /locus_tag="MJE33_002823"
     CDS             3350..5233
                     /gene="selB"
                     /locus_tag="MJE33_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HBY9648747.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNENSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIESGESGYVQLRLEESIVSKKG
                     DTFVVRRYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKRALETLISEDKVFIIGNMYMHINQYNKLKENTIKLLS
                     EYHKKYRLRKGILKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNDKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNILINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
     gene            5338..8076
                     /locus_tag="MJE33_002824"
     CDS             5338..8076
                     /locus_tag="MJE33_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HBY9648748.1"
                     /translation="MKNIIPDVKPDIKEREDMSLLFIIMGSVLLLFIIKDIEKYETLL
                     HAIYTIFLVATGVIIFNTTKFKVDSFSTFLGLIFIATGVLECIYLFNSLGIKTKSIME
                     INITISAITDLFPILGVYLSFKFVKDNKQIYSSVILFIATATLTITSLFIISGICDYL
                     GNGKSQYALGVIISIFIIIISIVSGIELNSSSNKGKWEYDEKKIFNRIIVIIILSRVP
                     NLLHIVIDNRNVERILSQIIINVALYYLYNYIVSKNIKKTALELHDTNEELTKKTESL
                     KEKNKKLIYETEKIEELKGILMKRESRLQSTLDVAVNSIVVFSKDGEITYANKSFRNT
                     FGDYKEQDKLTDKIKNFNDLIESIHYVFVHEKNAEKLISTSDNKVYQAIFAPLIIASQ
                     TEGVLCVLEDKTKKKEYERKLIEANKRSKDFLESIGDGIVVLEGNNKIYVNNACREIF
                     KNNLESIDFSLVCKSEESIEKRYVIDGEVKYVEMSFSQYTNAGTNKTIIVIRDTTSRK
                     ISQIKLEESQSSYSRFIDILPDGICLLKEDLSINYANKSFLDMLAFTNIDDIKDSNIK
                     LLMNANTEEKMKFTDNMAKVLNENRSILLLEYELINSYDDIVEVELSALPFAIYNTRY
                     IMLIIKDLVHKKYSEQAEKELLERFKTDKIKTEFFANMSHELKTPLNVISSSNQLVDS
                     FYRNEKIKDYNNNIKSHVDLVRQSSYRLQRLINNIIDLTKMESGFYTLKLAKYNIVSV
                     IEDLFMNIEEYALRKDIKILFDTDLEEINVYIDKVEIERIMLNLLSNCIKFTDNGGWI
                     YVSIHYKIDKVIISVKDTGVGIPQDKLELIFEEFSQVDKTLSRNTEGSGIGLAIVKNL
                     VSLHGGDIEVVSEVNKGTEFLISLPMKGFSSEHYTEDKRIYNIQEKIKIEFSDIYY"
     gene            complement(8195..9139)
                     /gene="manA"
                     /locus_tag="MJE33_002825"
     CDS             complement(8195..9139)
                     /gene="manA"
                     /locus_tag="MJE33_002825"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBY9648749.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGKYKGKKLSELWNKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVNMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNSGNKRELHVQKSIDVTNV
                     PHINFDTDYKIVSTSDFKCTTFVSNEFFSVYKLDVFGKCNFTHNTPFSLYSVLDGNGK
                     LIHNSVEYNLKKGMHFILPNDFGDFSFEGNLEIICSHI"
     gene            9312..10673
                     /locus_tag="MJE33_002826"
     CDS             9312..10673
                     /locus_tag="MJE33_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBY9648750.1"
                     /translation="MELFKAESKKYKKEFLKLLEQWVSIPSFYDRKTVSKDMPFGKGV
                     YDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSEPFK
                     LNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLDINLKRKVRLIAGGNEESGFKCIKH
                     YYSKEPYGVCGFTPDAKFPVLNGEKGGAIIKLISNIDDKSLYISGGIEFNTIPDKVYI
                     KNVEKLGKDNICFDINNISINYDNGNYIVYGKGGHSSKPEKSINPILATIKLLSENID
                     EKWTKDLYKLINQDNINGNLFGLNIEGKCGILSMVPTIINIVDGKLEVVLSVRYPEIL
                     TIEDIIKKFNLYMEQNNINKFELIGENLKQANYIDRNSKLVRSLHDIYIKYSGDLKND
                     VRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHSANEFASLDRFITAIGIYAEAIITLC
                     NKI"
     gene            10761..12650
                     /locus_tag="MJE33_002827"
     CDS             10761..12650
                     /locus_tag="MJE33_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBY9648751.1"
                     /translation="MITKKHAIIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIASSRGIGYKLSGSKNNIARAVKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIHGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIHCIESILEKHLREKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIENLMKEISDKIGFKLIEDEVIYISNYSGV
                     IAYKGTQGRKNTSEIEERISTVISSALQDIFLISGSDYTKDDEFMVAIFDHIKRFLNR
                     ARANVKSNNPLLHQIKEKFPIAFNLAVFLSNKLETEFNLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFSIKANTK
                     EEVIISLSNKLKERDFIEESSIKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            12665..13120
                     /locus_tag="MJE33_002828"
     CDS             12665..13120
                     /locus_tag="MJE33_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY9648752.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKDLNLAEDESLVY
                     EGLMAREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKEFKDSLLKSDNVEEISNLINEALGL"
     gene            13179..13496
                     /locus_tag="MJE33_002829"
     CDS             13179..13496
                     /locus_tag="MJE33_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBY9648753.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            13521..14783
                     /locus_tag="MJE33_002830"
     CDS             13521..14783
                     /locus_tag="MJE33_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBY9648754.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFVDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKRLVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAIWAWPLVDNLFGYIIGIAV
                     GAIFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            14888..15952
                     /locus_tag="MJE33_002831"
     CDS             14888..15952
                     /locus_tag="MJE33_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBY9648755.1"
                     /translation="MDKIEKVREYFREYDIDGFLINSPTNKFYVGNLFSSSGYVFITK
                     ESQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLRETKNEDEIKYIKKACEIVDATFYHIVDFIKVGMTE
                     KQVENEIVRIIKDLGGQKESFDTIVASGLRGALPHGKASEKVIEYGDFVTFDFGAKYN
                     NYCSDITRTICMGTINKELEEIYNIVRKANEECIRVLRPGMTTGEIDKVARDIIGSYG
                     YANNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITQDGCMRLTTSTKDLIVVS"
     gene            16006..17184
                     /locus_tag="MJE33_002832"
     CDS             16006..17184
                     /locus_tag="MJE33_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HBY9648756.1"
                     /translation="MYNFDDKPDRVSEKCRKWDLNIIRDKFGDIREDFIPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVNVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEVQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTRVATICKENNVILVADEVHAEHIHYGTFNS
                     ILKIGKELLENVILLTSPNKGFNLGGLKTSYSIVINKDIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLSGVNEYIKSNYELLETWVNKYNKIKLMKMESSYLAWMDISGL
                     GISASEFTDKLAIDTGVLLEDGSHFVKDGEKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
BASE COUNT         7065 a         1732 c         3107 g         5467 t
ORIGIN      
        1 tatttatttt aataatatca tcaataatcg tttataaatc gcttattttc aaagatgata
       61 tttatgttgt aagtaaaaaa aatttaccta atgaaaaaac acatgttgta gagaatgaaa
      121 ttaaaggtga agataataat cacagtaaag taactaaaga catagataaa aattttcaaa
      181 ataataaaat aactatatac ataagtggag cagtaaatag acctggaatt gtaactatag
      241 agtcagacaa gagactttat gatgctgtag aattactagg aggaacgaca aaagaagcag
      301 atttaaatgg agtcaatcta tcagtaagat tagaagatga acaacattac ataataccta
      361 aaataggtga agcaacatct gttacaagta atgatgattc caataaacca aatcagaaga
      421 atgagtcaag aggtgagcct aaaaataaat ccaataaaaa tatatctaac gaatcaaaag
      481 taaatataaa tgtagcgact atagaagagt tggattctct acctggggtt ggagaagcta
      541 cagccaataa aattttacaa cacagagaag aaaatggaca gtttagttca attgaggaaa
      601 taaaaaatgt aaatggtatt ggagataaaa agtatgaaaa tataaaagat ttaatttgtg
      661 tagactaaaa tataatacaa aacttacata tagatatgtt attataaata tgtatgtata
      721 tgtgtgtaca tgtggaaatt tagtaaagga ggcactttta tgaaaaaaga acaaaaaaaa
      781 cttacagaga tgacaacagc aggtggttga gccgctaaaa tagggccaga ggttctggca
      841 tcagttttat ctcaattgcc taaaaatgat aatatagaaa atttattagt aggactagat
      901 acagcagatg atgctgcagt ttataaatta aatgatgata tggcacttat ccaaacttta
      961 gacttcttta caccaatggt agatgaccca tacgtatttg gtcaaattgc tgcatcaaat
     1021 tcactatcag atgtatatgc tatgggaggc aaacctttgg ttgctatgaa tattgtttgt
     1081 tttccatcat gccatgatat ggatgtactt gcagaaatat taaaaggtgg gtttgataaa
     1141 gtgaaagaaa gtggagcact tttggttgga gggcatactg tagatgacaa agagcctaaa
     1201 tatggtcttt cagtttctgg gattgtaagt ccaaataaag ttttatcaaa tgctactgca
     1261 aaacctggtg ataagttaat tataacaaaa ccaattggag tgggtgtatt aaatacagcc
     1321 atgaaagaag gtatggtaga acaacatata gcagataaag taatagaaat aatgatacat
     1381 ttaaataaat atgcagcaat gagttttgag aaatttgatg taaattcggt tacagatata
     1441 actggttttg gtctacttgg tcatacttta gaaatggcca aagcttctga agtaagtatt
     1501 gaaattgaat caaaacatgt accaattatt gaaggtgcaa tagaaatggc acaaatggga
     1561 attatacctg ctggtatgta taaaaatatg cattatgtat ctaaagatgt agaagttgta
     1621 ggaaatatag aagttgcagt tcaagatatt ttatacgacc cacaaacatc aggagggttg
     1681 ttaatttcag taaaagaaga acttgcagag gagcttgtaa aagatatgaa attaaatgga
     1741 gcaatagaag ctaaaattat aggttctgta gttccaaaag gggaaaaata tattacagtt
     1801 ttataaaata ttatagagaa agagtgattg ttttgagcaa aagagaattg tttgcaatgt
     1861 tgccttcagt tgatgaagta ttaagtgata atagaattgt agaaataata aatgaatatc
     1921 ctagaagttt agttttagaa tctgtgagag aagtaataga tttaaaaaga caatttatat
     1981 taagattaaa agaagatgca tcaaactctg taacaataga gtttggtgaa ataatagaat
     2041 ctgcaataga gagagttaag ttaaattact cattatcttt aaaaaaagtt ataaatgcaa
     2101 ctggtacagt tattcataca aatttaggaa ggtctttact tagtgaagat ataaaagatg
     2161 aattatggtg tgctgcttca aggtattcaa acttagaata cgatttggat aatggagaaa
     2221 gaggttcaag atactctcat ttaactagta caataaaaag acttactgga gcagaagatg
     2281 tactagttgt aaataataat gcagcggcag tacttcttgt acttagcaca atggctaaag
     2341 gaggagaagc aattgtatct cgtggagaat tagttgaagt aggtggttca tttaggattc
     2401 caagtataat ggctcttagt ggtgcagaat tagttgaagt tggttcaact aataaaactc
     2461 atttgaaaga ttacaaggag gccattacag aagatacaaa tgtattgatg aaggttcata
     2521 caagtaatta tagaataatg ggattcacag agagtgtttc tatagaagaa ttggttaatt
     2581 taggtaaaaa atataaacta cctgtaatag aagacttagg aagtggagtt tttatagatt
     2641 tatcaaaata tggtttatct tatgaaccaa ctgtattgga ttctattcga caaggtgcag
     2701 atgtagtaac ttttagtgga gataagatgc ttggaggtcc acaagcaggt attatagttg
     2761 ggaaaaaaga gtatatagaa aaaatgaaga aaaatcaact tactagagca cttagagttg
     2821 ataagcttac aatatgtgct cttgaagcaa cattgagaat gtatttagat gagacaaaag
     2881 ctatagaaaa tatacctact ttaaaaatgt tgacatataa aattgaagaa ttagaagtaa
     2941 aagcaaataa actatttgaa aaaataactg ctttaaactt aaatgcaaac ataaatatag
     3001 aagatggatt ttcacaggtg ggaggagggt ctatgcctct tgaaactata agtacaaagg
     3061 taatatcaat aactcctgaa catatgaatg tttcatcttt agaaaaaaaa ctaagactta
     3121 gtgaagcaca tataatagcc agagtgtatg ataataaata tgtactagat gtaagaacta
     3181 tatttgatga tgaatttgat gtaatagttg aggaattgag aaaagcattt aattaaataa
     3241 atttgagtta ataaatatta aatttagaag tataagtatg aagttttgat taaaaaatag
     3301 ctacacacac ttgtgacttt agtcatagca ggtttagagg gggcaaaaaa tgaaaaatgt
     3361 agtaattgga acagcaggtc atattgacca cggaaaaact actcttataa aagcattgac
     3421 tggaagagag actgatacgt tagatgaaga aaaaaagaga ggtatttcaa taaacttagg
     3481 atttacttat tttgatttac caaacaatag aagagctgga attgttgatg tcccaggaca
     3541 cgaaaaattt ataaaaaaca tgttagctgg agcatcagga attgatatag ttttacttgt
     3601 tgtagcagca gatgaagggg ttatgccaca gacagttgag catttagata ttctaagctt
     3661 tttaaatatc aaaaatggaa tagtggttct aactaagtct gatactgtag atgatgaatt
     3721 tagagagcta gtaaaagaag atatttgtga aaaaataaaa ggaacttttc ttgaagatgc
     3781 tgaaataatt gaagttgatt ctatatctaa aagaggtata gacactctta ttgaaaaaat
     3841 agataaaatg agtgatgata tagaagataa aaatgaaaac tctccagcaa gacttaatat
     3901 agacagagta ttttcaataa aagggtttgg tactgtaata acaggcactt taatagaagg
     3961 aaagataagt atagaagatg atttggttat atatccacag gtattaaaaa caaaaataag
     4021 aagtatacaa gttcatggag aaaataaaga tacagcttat gcaggtcaaa gaacagcaat
     4081 caatatatca aatgtaaaag tggatgatat aaaaagagga gatgttttag ctgcaccaaa
     4141 ctcattggag gaatcaatga tgttagatgt aaaattatca ttggtaaatc atttagataa
     4201 aggattaaag cactgggata gattaagatt gtatcatgga actagagaga tactttgtag
     4261 agcagttcca ctagataaag aattaattga aagtggagaa agtggctatg ttcagttgag
     4321 acttgaagaa agcatagtat ctaagaaagg tgatacgttt gtagtgagac gttattctcc
     4381 aatggaaact ataggtggag gtgtaataat tgacccatct cctaaaaaac ataagaaatt
     4441 tgatgaaaaa gttatagaag cattaaaaat aaaagaaaaa ggcgaactaa aagatataat
     4501 agaagagtac ttaaaaagga atttaaaaaa ttatccaaat ataaaagaaa taatgagcta
     4561 tagtggtgct catgaagaag atgtaaaaag agcgcttgaa acacttatat cagaagataa
     4621 agtatttatt ataggaaata tgtatatgca tataaatcaa tacaataaat taaaagaaaa
     4681 tacaataaag cttttaagtg agtaccataa gaagtatagg cttagaaaag ggatactaaa
     4741 agaagaagta aggtctaaga tagagagtaa ttttaagact agggagatgg atattttatt
     4801 agaaaaattg tctacagaaa atgctataaa aatagaaaat aatatagtat ctctattaga
     4861 ttttgaggtt attttaaatg ataagcaaaa agaaatagca aaaaaaatag aaaaaagatt
     4921 aaaatcatgt ggagtttctt ctatactgac aatagatgaa gtttctgaag gaaatcataa
     4981 ctatgctgaa gtattggagt caatgatagg taataaagta gagaaattag atgatttgta
     5041 tataatggac aaagatattt atgaaaatgc aaagaacatt ttgataaatt atataaaaga
     5101 aaataaagaa ataacattag gagaatacag agatttgatt gattctagca gaaagaattg
     5161 catgattata ttggaaaact tcgatagaaa caaaataact aaaagagtag aaaacaaaag
     5221 aattttgttt tagtagaaaa tgtctgtttt tgctttaagg tataaagaag gaattataat
     5281 gtcgagtatc gtataattaa ttagtataaa atattaatta agaggaagtg taaagtattg
     5341 aagaatataa tacctgatgt aaaaccagat ataaaagaaa gagaagacat gagcctttta
     5401 tttataataa tgggctcagt acttttatta tttattataa aagatataga gaaatatgaa
     5461 actttattac atgccatata tacgatattt ttagtagcta caggagtaat tatatttaat
     5521 actacaaaat ttaaagtaga ttcattttct acatttttag gattaatttt tatagctact
     5581 ggagtattgg agtgtatata tttatttaat tccttgggga ttaaaactaa gtctataatg
     5641 gagattaaca tcacaatatc agcaataaca gatttatttc ctatactggg tgtatactta
     5701 agttttaaat ttgttaagga taacaaacaa atttattcaa gtgtaatttt atttatagca
     5761 actgctacat taacaataac atctttattc ataatatcag gaatttgtga ctatctggga
     5821 aatggtaaat cacaatatgc tttaggagtt ataatatcta tattcataat aattatttct
     5881 atcgttagtg gaattgaatt aaattcaagt tcaaataaag gtaaatggga atatgatgaa
     5941 aaaaagattt ttaacagaat aatagtaata ataatcttat caagagttcc aaatctattg
     6001 catatagtta ttgataatag aaatgtagag aggatattat cacaaataat aataaatgta
     6061 gccttatatt atttatataa ttacatagta tctaagaata ttaagaaaac agcactggaa
     6121 ttgcatgata cgaatgaaga attaacaaaa aaaacggaaa gtttaaaaga aaagaataaa
     6181 aaattaatct atgagacaga aaagatagag gaacttaagg gaatacttat gaaaagagaa
     6241 tctaggctac aatctacatt agatgtagca gtaaatagca tagtagtatt tagcaaagat
     6301 ggagaaatta catatgcaaa taagagcttt agaaatactt ttggagacta taaagaacaa
     6361 gataaattga ctgataaaat aaaaaacttc aatgatttga tagaaagcat tcattatgtg
     6421 tttgttcatg agaagaatgc agagaaactt attagtacaa gcgataataa agtttatcaa
     6481 gctatatttg cacctttaat tattgcttca caaacagaag gagtattgtg tgtactagaa
     6541 gataagacta agaaaaagga gtacgaacgc aaacttattg aagcaaataa gagaagtaaa
     6601 gattttttag aaagtattgg agatggcata gtagtgcttg aaggtaataa caaaatatat
     6661 gttaataatg catgtaggga aatttttaaa aataatttag agagtattga tttttcatta
     6721 gtatgtaaaa gtgaagaaag tatagaaaaa agatatgtaa tagatggtga agtaaagtat
     6781 gtagagatga gtttttctca gtatactaat gctggtacta ataaaactat tattgtaata
     6841 cgtgatacaa cctctcgaaa aatatctcaa ataaaacttg aggaaagtca atcatcatat
     6901 tcaagattta tagatatatt acctgatggc atatgcttat taaaagaaga tttaagtata
     6961 aactatgcaa ataagtcgtt tttagatatg ctagctttta caaacataga tgatataaaa
     7021 gattctaata ttaaactatt aatgaatgcg aatactgaag aaaaaatgaa atttacggat
     7081 aatatggcta aagttttaaa tgaaaataga agtatacttt tactggaata tgaattgata
     7141 aatagttatg atgatatagt agaagtagaa ttaagtgcat taccttttgc tatatacaat
     7201 actagataca taatgcttat tataaaagat ttagtacata agaaatattc cgagcaagca
     7261 gaaaaagaac tgcttgaaag atttaaaaca gataaaatca aaacggagtt ttttgcaaat
     7321 atgtctcatg aactaaaaac tccactaaat gttatatcta gttcaaatca actggtagat
     7381 tctttctata gaaatgaaaa gataaaagat tacaataaca atattaaatc tcatgtggat
     7441 ttagttagac aaagttctta taggcttcaa agattaataa ataacattat agatttaaca
     7501 aagatggagt ctgggttcta tacactaaaa cttgcgaaat acaatattgt atctgtaata
     7561 gaagacttat ttatgaatat agaagagtat gcacttagaa aagacataaa aattcttttt
     7621 gatacagacc ttgaagaaat taatgtttat atagacaagg ttgaaattga gagaattatg
     7681 ttgaatctat tgtcaaactg tattaagttt actgataatg gaggctggat atatgtaagt
     7741 attcattata aaatagataa agtaatcata agtgtaaaag acactggagt tggaatacca
     7801 caagataaat tagagttgat ttttgaagaa ttttcacaag ttgataagac tttatccaga
     7861 aatactgaag gaagtggaat agggcttgct atagttaaga atctagtaag tcttcatggt
     7921 ggagatatag aagtagtaag tgaagtgaat aaaggcacag agttcttaat ttcacttcca
     7981 atgaagggat ttagtagtga acattacaca gaagataaga gaatatataa tattcaagaa
     8041 aaaataaaaa tagaattttc tgatatatat tattaaaaat gattatgagc aaaaataaaa
     8101 taaaaatact atttgtttaa atatttaagt tgtaagaaat aggctatcat aaggtaaatt
     8161 attatgatag cctattttta gctaggtttt atatctatat gtgagaacat ataatctcta
     8221 aattaccttc aaaactaaaa tctccaaagt cgtttggtag tataaaatgc ataccctttt
     8281 ttaaattgta ttctacagaa ttgtgtataa gttttccatt tccatccaac acactgtata
     8341 aagaaaaagg tgtattgtgt gtaaaattac attttccaaa aacatctaat ttataaacac
     8401 taaaaaattc gtttgacaca aatgttgtgc atttaaagtc tgatgttgat acgattttat
     8461 agtcagtatc gaaatttata tgaggaacat tcgtaacatc tattgatttt tgaacatgaa
     8521 gttcacgctt attacctgaa ttgtcagttc tatcataatc atagactcta taagtagtat
     8581 ctgagttttg ttgagtttct aaaatcaatg ttcctttgca tatagcatgt atagtgccac
     8641 ttggaacata gaaaaagtct ccctttttta tattaacctt tctaagtagt ttgtcccatt
     8701 cattattatt taccatattt acaaattctt tatgagattt tgcattatga cctattataa
     8761 tttctgcatc atcagaacaa tctattacat accaacactc tgtcttacct aactctccat
     8821 tttcattttt tttagcatat tcatcatttg gatgaacttg aactgataaa ttatcatttg
     8881 catccaatat ttttgtaagt aaaggaaact tatcaccagg tgtgtttcca aataattctc
     8941 tatttttatt ccacaattca gatagttttt tacctttata ttttccattt tctattaagc
     9001 aatctccatt tttatgagag cttatagccc aacattcacc agttgttggc gaatctattt
     9061 cataattaaa tttatcttta agtgcagtac caccccatat tctatccata aaaataggtt
     9121 ttaaaaataa tggttccata atcctgttcc tcctatgata ttaaatttat cctacaaata
     9181 attatatata aatagattat aaaatcaata gtaaaaaagt tttgtccctt gaaatgacat
     9241 aaagtataag gagttattaa ataagataaa ctatgatata taatataaag ttctaaattg
     9301 gagtgataat tatggagttg tttaaggcag aaagcaaaaa atataaaaaa gaatttttaa
     9361 aattattgga gcaatgggtt agtatacctt cattttatga tagaaaaact gtatctaaag
     9421 acatgccttt tggaaaagga gtatatgatg cattaaactg gtttgaaaat ctaggtagag
     9481 aaaacaattt caaagtaaaa aatatagata atcatgctgt tcaaattgaa tatggaaatg
     9541 gtaaagagta tgtagatata tttggacatt gtgatgttgt aaatccaggt gaaggatggg
     9601 atagtgaacc atttaaacta aatattatag gtgataaatt agttgctaga ggtgtatctg
     9661 ataataaagg gcctatgata gtgaattttt tagcactaaa aatgataaag gatttagata
     9721 ttaatttaaa aagaaaggtt agattaatag ctgggggaaa tgaagagagt ggatttaaat
     9781 gtataaagca ttattatagc aaagagccat atggtgtatg tggatttact ccagatgcta
     9841 aatttcctgt attaaatgga gaaaaaggtg gagcaataat aaagttaatt tcaaatatag
     9901 acgataaatc tttatatata agtggtggaa tagagtttaa tacaatacca gataaagttt
     9961 atataaagaa tgtagaaaaa ttgggtaaag ataatatatg ttttgatatt aacaatatat
    10021 cgataaatta tgataatggc aattatattg tgtatggaaa aggtggtcat tcatctaaac
    10081 ccgaaaaatc aataaatcct atattagcta caattaaact tttgtcagaa aatattgatg
    10141 aaaaatggac aaaagattta tataagttga taaatcaaga taatataaat ggaaacttat
    10201 ttggtttaaa tattgagggg aaatgtggaa ttttatcaat ggtgccaaca ataataaata
    10261 tagtagatgg aaaattagaa gtagttttaa gcgttagata tccagaaata ttaaccattg
    10321 aagatataat aaaaaaattt aatttgtata tggagcaaaa taatattaat aaatttgagc
    10381 ttattgggga aaatttaaag caagcaaatt atatagatag aaattcaaaa cttgtaagaa
    10441 gtttgcatga tatatatata aagtattctg gagatttaaa aaatgatgtt agagtaacta
    10501 gtgcaggaag ttatgcttct gaaatgaata attcagtaat atttggttgt gaatttcctg
    10561 atggtagctt tggcaatgtc catagtgcga atgaatttgc tagcttagat agatttataa
    10621 ctgcaatagg tatatatgca gaggcaataa ttacattatg taataaaata taaatacaaa
    10681 atgtccctta tatagacata ttatatatac aattaaaatt ttacttgata tataattgaa
    10741 atcaaagaga aggtgataca atgataacta agaaacatgc aataatagta aagagtttaa
    10801 gcaataaaga tggatatatg acatcaaacg aattagctat caaattggat gtgtctacta
    10861 aaactattaa aagatatata gcagatttaa atagtgtttt aagcaaatat gatttggtaa
    10921 tagcttcttc tagaggtata ggatacaagt taagcggttc taaaaacaat atagccagag
    10981 ctgttaaaga agcaaataaa tatattgatg gttttttaga tgactctgaa gagtcaagaa
    11041 tgtcaaatat aatatgtatg cttattaata gaaattatat gagtatagaa gcaatggcag
    11101 aagaattaaa tctaagtata gccgctataa ataaattgtc tagtaagctt aaaagaaaac
    11161 ttgaaaagta tgacttagtt ataaaatcta aaccttacta tggaagctat atacatggtg
    11221 aagaaattaa tataaggcaa ttaattactg actatgcaat aaaattagat gaaaaaagta
    11281 aagtgaaggt gtttttagat gacattagtg aaaacgatat acactgtata gaaagtattt
    11341 tagaaaagca cttaagagaa aaagatacaa taatttcaga taaagatttt aatcttttgc
    11401 tttcaaaaat aatcgtttct gtatttagga gtaagagagg tcatagtaac aatataaact
    11461 taatggatac aagttataga tttcataact actactttat agaaaatcta atgaaagaaa
    11521 tatctgataa aattggattt aaacttattg aagatgaagt aatttatatt tcaaattatt
    11581 ctggtgtaat agcctataaa ggtacacaag gaagaaaaaa cacaagtgaa attgaggaac
    11641 gaataagtac tgttataagt agtgcacttc aagatatatt ccttatttca ggtagtgatt
    11701 atacaaaaga tgatgagttt atggttgcaa tctttgacca tataaagaga tttttaaata
    11761 gagctagagc taatgtaaaa tcaaataatc ctttacttca tcaaataaaa gagaagtttc
    11821 ctatagcttt taatttagcc gtgtttttat caaataaact agaaactgag tttaatttaa
    11881 aacttgatga agatgagctt ggatatatag ctatccactt tgctgcttca aatgaaagaa
    11941 tgaaaaaaaa tactagtaag aaaatttgta tagtttgtca ttatgggata ggaacaggac
    12001 aacttatttc tgaaaagcta aaacagaata taagtgattt aagtgttgtg ggtgtttatc
    12061 cagttagata tctcgatatg gctataagtc aagatgttga tttgatagta tctaccgttg
    12121 aattaaaagg atatgaaaaa cctgtcttat atatagaaaa tatatttgat gatagcttaa
    12181 ttgaaaacgt aaacaaagcc ttttatgaaa aggaagaaag aagaaagata ataagcaaca
    12241 tgtttgatga aaaagctttt ttcagtataa aggctaatac taaagaagag gtcataattt
    12301 cactttcaaa taaactaaaa gaaagagatt ttatagagga aagttctata aaaagtataa
    12361 tagatagaga aaatatttca tcaactgaaa taggaaatct agtagcaata ccccacacaa
    12421 tagtgaaggg agataaaaaa tctataatag gagttggaat acttgaaaat ccaataattt
    12481 gggataagca agaggttcag ctagtattta tggtattttt taatactaaa gaaaaacata
    12541 atttctcgat atttaaatac ctttataatt ttataaaaga tgaaggcgga gtaagaggaa
    12601 ttattaaaat atgtgacttt aataaattaa tggcactgat aggtaattaa ggaggaaatt
    12661 aataatggga acaaacatat ttaacaaaga atatgtattt ttaaatgttg atgctaagtc
    12721 aaaggttgaa gttttaaaat ttatatcaaa aaaagctaaa gacttaaact tggcagaaga
    12781 tgagagctta gtatatgaag gtttaatggc tagagaagaa caatttacta caaatttagg
    12841 agaatctata gcaatacctc acactaaaaa tgatgcaatt gaaaatccag cagtagtagt
    12901 attgaagttt aatgaagatg ttgtttggaa tgaaggtgag gataaggtta aattagcaat
    12961 aagcttactt atgccaggaa aaagtaaaga gaatattcat cttaaattat tatcatctct
    13021 ttcaagaaaa ttaataaata aagaatttaa agattctctt ttaaaaagtg ataatgttga
    13081 agaaatatct aacttaataa atgaagcttt aggtttataa ttaaacacta aggttataaa
    13141 ataaaaaaat aattaactaa aaataggagg aatttgaaat gaataaaaaa ttagtagcat
    13201 tatgtgcttg tccaatggga ctagctcaca ctttcatggc agcagaagca atagagcaag
    13261 cagcaaaggc attaggttat gaagcaaaag ttgaaactca aggtgctgat ggtgtacaaa
    13321 atgagttaac aagagatgat atattaggag caactatgat aatacatgca gtagcaataa
    13381 caccagaagg tatggaaaga tttgatggtt gtgaagttta tgaagttgaa ttacaagaag
    13441 ctataaaaaa tgcagaaggt gttataaaag aaatagaaga agatttagga atataataaa
    13501 tttattgtgg aggaaataga atggcaatta agaaaaaagt tataaacagt tcaggtgctt
    13561 ctacaacagg aggaaataat gtaggaaaat ctactccatc tagtccaact caatcaaagg
    13621 gaaatggaaa atggaaagaa ataagtaaac atataatgac aggtatatca tatatgatac
    13681 cagttttagt aatgggtgga cttatagggg ccttatctca attaatacca tatgctatat
    13741 taggacttga cccatctgta ggtatagttg atgctatgaa ctcaggagaa tttacagggt
    13801 tcaagcttag tcttttaaat atagcacaat taatgtcaaa tttcggattt actttgtttg
    13861 gatttgcgat accactgttt gcagcatttt gtgcaaactc tataggtgga aaaacagctt
    13921 taattgcagg atttataggt ggatatatag ctaataaacc tgtaggtgta ccacaattcg
    13981 ttgatggaca atggactgaa gttgttccag ttgcatcagg atttttaggt gcaatactaa
    14041 tagcatttat aataggatat tttgtaaaat ggttaaataa atctataaaa gtttctcata
    14101 actggttagc ttttaaaaca acatttttaa taccattaat agcgtcatta gcttgtatgg
    14161 tattgatgat atttataata actccatttg gtggattaat taatgaaagt atgaaaaact
    14221 tcttaacagc agcaggagca gctggagaat atgtatatgc aacagctttg gcagcagcta
    14281 cagcattcga cttaggagga ccaataaata aagcagcagg attcgttgca cttggtttaa
    14341 ctacagagaa tgtacttcca ataacagcaa gaacaatagc tatagttgtt cctccaattg
    14401 gattaggatt aacaacatta ttagataaaa gattagtagg aagaagagta tatgatagac
    14461 aattctatca agcaggaaaa acttctatat tcttatcatt tatgggtata tctgaaggag
    14521 caataccatt tgcacttgag agaccaggtt ttgttatacc tttaaacata gttggaagtg
    14581 taataggagc tattacaggt ataattctag gagcaattca atggttccca gagtcagcta
    14641 tatgggcatg gcctctagta gataacttat ttggatatat aataggtata gcagttggtg
    14701 ctatattcat agcagtagga aatatattct atagaaataa attaataaaa gatggtaaac
    14761 ttgttgtaga ttatatcgac tagtatttaa tttttactat ctataaataa ctttggctgg
    14821 taatatggtt ttaatcaata ttaccagcct tacctatatg attaattaaa ggcggtggag
    14881 ataagatatg gataagatag aaaaagtaag agagtatttt agagaatatg atatagatgg
    14941 atttcttata aatagtccta caaacaagtt ttatgttgga aatttattta gctcttcagg
    15001 ttatgttttt ataactaaag aatcacaata tataatagta gattttagat attttgaaga
    15061 aattaaaaga aaatctagtt tgtttaatgt agtattaatg gacaaaacta gaactcattt
    15121 tgatataatt aatgatattt gtagagagca gaatataaag gaaattggat ttgagggaaa
    15181 tgaagtatct tttgatttat atagaagtat gagtaataag ttaagtgcaa ccttaaaatc
    15241 agttgattta tctacattaa gggaaacaaa aaatgaagac gaaataaaat atataaaaaa
    15301 agcttgtgaa atagttgatg ctacatttta tcatatagta gactttataa aggttggtat
    15361 gactgaaaag caagttgaaa atgaaattgt tagaattata aaagatttag gtggtcaaaa
    15421 agaatctttc gatacaatag ttgcatctgg gcttagagga gcattacctc atggaaaagc
    15481 aagtgaaaaa gttatagagt atggagattt tgttaccttt gactttggtg ctaagtataa
    15541 taattattgt tcagatatta ccagaaccat ttgtatgggt actataaata aagagttaga
    15601 agaaatatat aatatagtaa gaaaagctaa tgaagagtgt ataagggttt taagaccggg
    15661 aatgactact ggagagatag ataaagttgc aagagacata attggttcat atggatatgc
    15721 caataatttt ggtcataatc tgggtcatgg tgtaggaatt atggttcatg aatatcctgc
    15781 attagctcct gaatcaaatg aagtattaaa agaaggaatg attgtaacta tagagcctgg
    15841 tatatatgtt ccaagtcttg gaggcgtaag aatagaagat gatgtactta taacacaaga
    15901 tggatgtatg agattgacta catctactaa ggatttaata gttgtaagct aaaaataaat
    15961 tagataaata ttttataaca ataagaaaag aagaggtaaa tttaaatgta taattttgat
    16021 gataaaccag atagagtaag tgaaaagtgt agaaaatggg atttaaatat tataagagat
    16081 aaatttggag atattagaga agattttata ccaatgtgga ttgctgatat ggattttaaa
    16141 ataccaacag aaatagaaaa caaatttatt gaagctgtaa gaagaggtgt atttggatat
    16201 acatactgtt atgatgaatt ttatgattca gttattggtt ggcaaaaaaa tatgcatgag
    16261 gtaaatgtag aaaaagaatg gattacatta acttatggaa ctgtatcaac acttcattat
    16321 gtagtacaag ctttttgtaa gaaaggtgat agtataatat taaatacacc agtttatgac
    16381 ccctttgaat catcagcaaa aaaacaaggt gtaaatgtaa tttgtaatac attagatgtt
    16441 gtaaatgata gatattatat aaattttgat aaattagaag ttcaaattaa agaaaataag
    16501 cctaaattaa tgatgttttg tacaccacat aatccatctg gaaggatatg gacaattgaa
    16561 gaaatgacaa gggtagcaac tatatgtaaa gaaaataatg ttattttggt tgcagatgaa
    16621 gtacatgcag agcatattca ttatggtaca tttaattcaa tacttaagat tggaaaagag
    16681 cttttagaaa atgttattct attgacatca ccaaataaag gatttaatct tggtggatta
    16741 aagacttctt attcaatagt tataaataag gatataaggg acaaatttag aaataaatta
    16801 aagcaaaatt ctataacttc tccaaatgtg tttggaataa taggattgat aaccgcttat
    16861 aatgaatgtc atgagtggtt aagtggtgta aatgaatata taaaatcaaa ttatgaactt
    16921 ttagaaactt gggtaaataa atataataag ataaaattga tgaaaatgga atcatcttac
    16981 ttagcttgga tggatataag tggattaggt attagtgcaa gtgagtttac agataaattg
    17041 gctatagata caggagtatt attagaagat ggaagtcatt ttgtaaaaga tggtgagaag
    17101 tatgttagaa taaatttagg aactcaaaag gaaaatgtaa ttgaagcctt aaatagaatg
    17161 gatttatttt taaagtcttt ataaaaattt ggttgaatta gtattttata taaatttaaa
    17221 ctattaatat ggtatataca taagtagaat ttgttttaga ttacttaagt atataccata
    17281 ttttatataa aaattaaatt tgcattagtc atgtgataac tatttaaatt catatgtatt
    17341 ttgtatgtat aatagatata attattcata t
//
LOCUS       DAGSRM010000083        17249 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.083, whole genome shotgun sequence.
ACCESSION   DAGSRM010000083 DAGSRM010000000
VERSION     DAGSRM010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17249
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.083"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            69..1373
                     /gene="gcvPA"
                     /locus_tag="MJE33_002833"
     CDS             69..1373
                     /gene="gcvPA"
                     /locus_tag="MJE33_002833"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013239064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HBY9648757.1"
                     /translation="MLKVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKR
                     LSEENLSLEDLTCFLGAGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFE
                     FQSMIAEITGMEIANASMYDGATAAIEACIMAMNQTRKSKIVVSKTIHPETLSVLRTY
                     LQYKDCEIVEIDFCNEYGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITH
                     ENKAMLIMSVDPISLGVLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTR
                     KMPGRIVGQSLDVEGKIAYVLTLQTREQHVRREKATSNICSNQALNALVASIYMATMG
                     KEGFKEVGMQSMKKAHYTYNKLVQTGKYKPIFKGKFFKEFAVQGNLNIETINDKLLEE
                     NILGGYNLEYNYPELKNSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            1373..2830
                     /gene="gcvPB"
                     /locus_tag="MJE33_002834"
     CDS             1373..2830
                     /gene="gcvPB"
                     /locus_tag="MJE33_002834"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HBY9648758.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIEDMIDPDMARQSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLAEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRGDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINVLKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGDMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPIPVIERKEDKYVLNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIEAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
     gene            3253..5610
                     /locus_tag="MJE33_002835"
     CDS             3253..5610
                     /locus_tag="MJE33_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBY9648759.1"
                     /translation="MDKVNLERYSCDINVGLNKNQLQSRIDDNLINAFDNGKTKTYKQ
                     IFKDNIFTLFNLINIVLVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLS
                     VIVSNKVSVIREKEKKEIDVQELVLDDIMILKTGNQICADSKVLNGKLEVNESLVTGE
                     SDIIIKNKGDFLYSGSFVVSGEAFVRVENIGKDNYANKIANDAKISKKHNSQLRNSLN
                     AILKIVGIIIIPLGIILFAKQHFGNGDSIATSVVGTVAAVNGMIPEGLTLLTSIALAV
                     GTIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDIVMLEEVDINEIMG
                     NICNSLKDDNATLNAIRDKYNVLDTYKAKNLIPFSSERKYSGVTFENKGTYLLGAFEF
                     IFKEKNKKLRIEVERYSKKGNRVIVLAHSDSYMEDDNIPEDIKPLAFILILDKIRDEA
                     KETLEFFYNEGVDIKIISGDNPITVSEVARKAGLKTASNFIDATTLKNDSDIYKAVDK
                     YSVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNV
                     SNLVLLDSNFASMPKVVMEGRKVINNIQRASSLFLVKTIFSFFLSLLTLFFFSRQYPF
                     VPIQLSLIGAVTVGIPSFFLALEANKSRISGNFLLNILKNALPGALCVTVNVIIVYNI
                     VEYLGYSSSVFSTMCAILTGVCGLLILRQQCLPFNKERLFLIVSMTIAFVIGILFLGG
                     LFSFVALSRELILITMALAILMPIMIKVMLFVLDEILETIVPDLN"
     gene            complement(5696..6013)
                     /locus_tag="MJE33_002836"
     CDS             complement(5696..6013)
                     /locus_tag="MJE33_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HBY9648760.1"
                     /translation="MSEFKNVTAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEMLAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
     gene            complement(6597..7868)
                     /locus_tag="MJE33_002837"
     CDS             complement(6597..7868)
                     /locus_tag="MJE33_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBY9648761.1"
                     /translation="MLKVLKGNIIFTKTSETFTVFENSYIILEKGKVKGIFKEIPKEY
                     KNLKVVDYTDKLIIPGMNDLHAHASQFKNLGMAMDKELLPWLETYTFPEESNYKDIEY
                     ATKMYKKFVKELWKSGTTRIAVFATVHKEASMKLMDIFKEVGLGAYVGKVNMNMNCPD
                     TLLENTQDSITDTEEIINKYSDTDSIVKPIITPRFIPSCTSELLKGLGDLCLKYNIPI
                     QSHLSENYDEIEWVRSLEPESKFYGDAYNRYNLFGQTPTLMAHCVHCSQDEIDLMREN
                     NVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHTLSIFKAMVSAIQLSKL
                     YWVNSGKKYNFLSLSETFYIATKSGGSFFGKVGSFEEGYDFDALIIDDSNLNHDNYSI
                     LERLERFIYVGDDRNIIHRYVCGKLIEEPNI"
     repeat_region   8515..9666
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=8515..8543
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaat"
     gene            10075..10362
                     /locus_tag="MJE33_002838"
     CDS             10075..10362
                     /locus_tag="MJE33_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648762.1"
                     /translation="MKKFLYALYSFIVIIANFRLKEKNYNFILLAIFFILLLIQNGVL
                     KTGYQNRATFYQDNYFNGEDKNDKIKFMTIKIFIFLSLPLCFNILFNYLTS"
     gene            complement(10901..11452)
                     /locus_tag="MJE33_002839"
     CDS             complement(10901..11452)
                     /locus_tag="MJE33_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY9648763.1"
                     /translation="MSINTVIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SIHTYHNQGEKTLKAVIINYYPV"
     gene            11643..12605
                     /locus_tag="MJE33_002840"
     CDS             11643..12605
                     /locus_tag="MJE33_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBY9648764.1"
                     /translation="MRVLNNVTEAIGNTHMLHLSKIADEFGVQGNIYAKMEYLNPGFS
                     KKDRVALQMITEAENAGLLVPGQAVVELTSGNTGTGLAIACACKGYKFIACMSKGNSM
                     ERARMMKALGAEVVLVDQMPNSVHGQVSGEDLALVEIEAQKIAKERNAFRADQFNLEA
                     CNHAHEKYTAEEMWEQLDGNIDVFVDFLGSGSTFAGCSKTLKKHNSSIKCYVVEPETA
                     AIYSGKEITDQGHKIQGGGYSMDLPLVDKKLIDGYIQVTDEEATDVARKLAKLEGVFA
                     GFSSGANVCAAIKLLKSSEKGKNIVLTLNDSGLKYLSTDLYEEL"
     gene            12899..14743
                     /locus_tag="MJE33_002841"
     CDS             12899..14743
                     /locus_tag="MJE33_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBY9648765.1"
                     /translation="MQKRILRNFIFTGKNILSKLVFIIKYNFSTLILFELFHKGLALF
                     FIWPSIRYIFDILIKSLHIVYLNMDNFIKVLTNPMSIVLIILSVIILAFYAFFEFTSV
                     IICFNKSIKYEKIGLFELFKISFENSIKLLYPKNILLFIFVILIIPLVNTVLISGFIG
                     KIQIPEYILDYIKSDIILNIIYMIFLLIIYISVIRWIFSIHEITLNTENFKRARKESV
                     NLIKGKIIRTIIYSLILSLVVAIIGYAIYHSGIIFIGIWTKYCTDMEFLKNVFINRCI
                     AFEEYFRLILSIFIFILDIGFISATYYQYKGVSISKNNIKKEKKSISKIVFNLVLILL
                     ISYIESVAFSSHINGMFNTSFSFYTTAIAHRAGAEMAPENTLAAIEEASHAKADYAEI
                     DVQETKDGELIVLHDSNFKRTTGVDKNVWDVNYNEVKTYDAGSFYYYGKGYYDEKFVG
                     EKIPTLKEKIKYSKGKVKLLIEIKLNGHEKGDVVKKVVQLIKENNFENQCIVASMDKT
                     ILQKVKKLDENLLTCYFMAIAYGDFSKLNYVDIYGIESTFVNKTVVEKIHKMNKKIFV
                     WTVNSDSLIEKMLDLNVDSIITDNPYLIESAIYWKKNGFISQVSDYLF"
     gene            15065..15649
                     /locus_tag="MJE33_002842"
     CDS             15065..15649
                     /locus_tag="MJE33_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBY9648766.1"
                     /translation="MTEREKMLAGELYDCGDIELLTQWHKAKDLVRDYNQTDSSDLEK
                     KEKILNELLGGKGKNLWITAPYYVDYGNNIYFGNNCEVNMNCTFLDDNKIIIGDNALI
                     APNVQIYTAFHPTNAGDRFGNAKEDGSFEFCKTQTAPVTIGDNVWIGGGAIIMPGVTI
                     GDNVVIGAGSIVTKDIPSNMIAYGNPCRIIRENK"
     gene            complement(16752..16928)
                     /locus_tag="MJE33_002843"
     CDS             complement(16752..16928)
                     /locus_tag="MJE33_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648767.1"
                     /translation="MFIGEIDKCTHILTAYISSSYDYCNFLDTQLDDFISEYGETVVE
                     TCLHQVLLLVSRYN"
BASE COUNT         6645 a         1825 c         2629 g         6150 t
ORIGIN      
        1 ataatattat attaaacaaa gaaaataaat tttcatatat acctgctaca agtgaagata
       61 aatcaaaaat gttaaaagta gttggattaa attcagttga tgaattattt tcagatatac
      121 cagaagaagt aaaattaaaa agagatttaa atttggaaat tggaaaatct gaattagagg
      181 taagcaaaat agtaaaaaga ttgtcagaag aaaatttaag tttagaagac ttgacttgtt
      241 ttcttggtgc tggtgcatat gaccattata taccatcaat tataaaacat ataacttcaa
      301 gatctgaatt ttatacagca tatactccat atcaagctga aataagtcag ggaactttac
      361 aagtagtttt tgaatttcaa tctatgattg cagaaattac aggtatggaa attgcaaatg
      421 catcaatgta tgatggagca actgctgcaa tagaagcatg tataatggca atgaatcaga
      481 ctagaaaaag taaaatagta gtgtcaaaaa ctattcatcc tgaaactctg tcagtattaa
      541 gaacttactt acaatataaa gattgtgaga tagtagaaat tgacttttgt aatgaatatg
      601 ggactacaga tatagaaaaa ttaaaagctt ctgtagacaa agatacagca tgtgttctta
      661 tacaaactcc taatttcttt ggaattattg aagaaatgga agaaatagaa aagataactc
      721 atgaaaataa ggctatgtta attatgagtg tagacccaat atcattagga gttttaaaaa
      781 caccaggtga gataggtgca gatattgttg ttggagaagc tcaatcactt ggaaaccctc
      841 ttaattttgg aggtccttat gtaggatttt tagctagcaa atctaaatat actagaaaaa
      901 tgccaggaag aatagttggg caaagtttag atgtagaagg taaaattgca tatgtattaa
      961 ctttacaaac tagagaacag catgtaagaa gagaaaaagc aacttctaat atatgttcaa
     1021 atcaagcttt aaatgctctt gtagcttcaa tatatatggc tactatgggt aaagaaggat
     1081 ttaaagaggt tggtatgcag tctatgaaaa aagcacatta tacctacaat aaacttgtac
     1141 aaactggaaa atataagcca atatttaaag gtaagttttt caaagaattt gctgtacaag
     1201 gaaatcttaa tatagaaact ataaatgata aactcttaga agaaaatata ttaggtggtt
     1261 ataatttaga atacaattat ccagaattaa aaaattcaac tttgctttgt gtgactgaga
     1321 aaagaagcaa agaggaaata gataagttag ttggaataat ggagggatta taatgaaaga
     1381 atacaatagt ttgttgatag atatatctaa aaaaggtagg aaagcatact cacttccaaa
     1441 gttagatata gatgatataa aaatagaaga tatgattgac ccagatatgg caagacaaag
     1501 tgagttgaat ttgccagaag ttggtgaatt agaattagtt agacattata cattattatc
     1561 aaataaaaac tttggagttg atacaggttt ttatcctttg ggttcttgta ctatgaaata
     1621 taatcctaaa ataaatgaag atatggcagc acttccaaac tttactggaa tgcatccata
     1681 ccaatcttcg gatacagctc aaggttctct ttctttgatg tatgatttat caagaagact
     1741 tgcagaaatt acaggtatgg atgaagttac actacaacct tcagcaggtt ctcatggtga
     1801 gtttacaggg cttatgatta taaaagcata tcatgaaaat agaggtgacc ataagagaac
     1861 caaagtgata attccagatt ctgcacatgg aacaaatcca gcaagtgcag ctatggcaaa
     1921 ctttgatgtg attcaaatag catctgataa aaatggagct gtagacataa atgtattaaa
     1981 agaggttttg aatgatgaag tagcagctct tatgcttact aatccaagta ctcttggttt
     2041 atttgaaaaa aatataaaag aaatagctac tcttgtacat gaagcaggag gacttttgta
     2101 ctatgatggt gcaaacatga acgctattat ggggataaca agacctggag atatggggtt
     2161 tgatgtggtt catcttaatc ttcataagac tttttcaact cctcatggag gtggaggtcc
     2221 aggagctggt cctgttggag ttaaaaaaga attagtacct tttttaccaa taccagtaat
     2281 tgaaagaaaa gaagataagt atgtactaaa ttatgataga gaaaaatcta ttgggaaaat
     2341 taaaaatttc tatggtaatt ttggagttct tgtaagagct tacacatata tactaactat
     2401 gggaagggat ggacttaaag aggctagtga aatggcagtt ttaaatgcta attatataaa
     2461 ggaaagtata aaagatgatt atattttgcc aatagatacc ttgtgtaagc atgagtttgt
     2521 attaggtgga ttgccaagag gagaggccaa tataaaaact atcgatattg caaaaagact
     2581 actagattat ggataccatc ctccaacaat gtactttcct cttattataa atgaggcact
     2641 tatgatagaa cctacagaaa gtgagagtat agagacatta gatagtttta tagaagctat
     2701 gaaaagtgtt gctaaagagg caaaggaaga accagaacta ttgaaaactg ctccacataa
     2761 tactttggtc aaaagagtag atgatgcaag ggcagtaaag aaacctatac taacatggtc
     2821 acaaagataa atagttaaaa agtagagatt taaagtataa agagaattaa atttataaga
     2881 aaaagctatc taacttaatc tagtttagat agcttttttg tatattagta tatatggtat
     2941 ttcctatata aagttttaaa ttatattagt gcttatatag aatattgata tgatgattat
     3001 gtatagatat taataagaat tgttgaattt atcttgcctt aactttattt actcttttat
     3061 attaagaaaa acttaactaa aatatagcga aattcataaa aatttaggaa atcttaagaa
     3121 ttataatatt taaatcttta gatttatatt ttattatata aactatagaa taataaaata
     3181 aaaaaattga gaattttata atatatttgt agatttgagt ataaaaaagt gctaagttga
     3241 aaggagtaaa atatggataa ggttaattta gaaaggtata gttgtgatat aaatgttggt
     3301 ttaaataaaa atcaacttca aagtagaata gatgataatt taattaatgc atttgataat
     3361 ggaaaaacca aaacatataa acaaattttt aaagacaata tatttacact ttttaatctc
     3421 ataaatattg ttttagtctg tttattagtt tcagttggtt cttttaaaaa taccttattt
     3481 atatttattg ctgtaattaa tactattata ggagtaatac aagagataag agctaaaaga
     3541 ttattggaca atctatctgt tatagtttca aataaggtat ctgtcataag agaaaaagag
     3601 aaaaaagaga ttgatgtaca agaattagta ttagatgata ttatgatttt aaaaacaggt
     3661 aatcaaattt gtgcagattc aaaagtttta aatggaaaat tagaggtaaa tgagtccttg
     3721 gttacaggtg aatctgatat tattataaaa aataaaggcg attttttata ttctggaagt
     3781 tttgtagttt ctggagaagc ttttgtaaga gttgaaaata taggaaaaga taactatgca
     3841 aataagatag ctaatgatgc aaagatttct aaaaaacata attctcaact tagaaattca
     3901 ttaaatgcaa tattaaaaat tgtaggtata ataataattc ctctaggaat tatattgttt
     3961 gcaaaacaac attttggaaa tggagattct atagctacat ctgtagtagg tacagtggca
     4021 gctgtaaatg gtatgatacc agagggatta acattactta cgagcattgc tctagctgta
     4081 ggaactataa aacttgctag tcataaaact ttagtgcaag aactgtactg tgtagagact
     4141 cttgctagag ttgatacact atgtcttgat aagacaggta caattacaga aggtaaaatg
     4201 caagttgatg atatagtaat gttggaagaa gtggatatta atgaaataat ggggaatata
     4261 tgtaattctc tcaaagatga taatgcaaca ctaaatgcaa taagagataa gtataatgtt
     4321 ttagatacgt ataaagctaa gaatttaata ccattttctt cagaaagaaa atacagtggg
     4381 gttacctttg aaaataaagg tacatattta ttaggtgcat ttgagtttat atttaaggaa
     4441 aaaaataaaa aacttaggat agaagttgaa agatattcta aaaaaggaaa cagagtaata
     4501 gttttagctc atagtgattc ttatatggaa gatgataata ttcctgaaga tataaagcca
     4561 ctagctttta tattgatatt ggataaaatt agagatgaag ctaaggaaac tttagaattt
     4621 ttttacaatg aaggtgtaga tataaaaatt atatctggtg acaatcctat aacagtaagt
     4681 gaggtagcta gaaaagcagg tcttaaaaca gctagtaatt ttattgatgc aacaacactt
     4741 aaaaatgata gtgatatata caaagcagtt gataaataca gtgtttttgg aagggtaagc
     4801 ccacaacaga aaaaacaaat gattttagca ttaaaaaaac aaaatcatac agtagctatg
     4861 actggagatg gtgtaaatga tgtgctagca cttaaagaag cggattgtag cattgccatg
     4921 gcatctggaa gtgatgttac taagaatgtg tctaatttag tcttattaga ttcaaacttt
     4981 gcatcaatgc caaaggttgt tatggaggga agaaaagtaa ttaataacat tcaaagagca
     5041 tcttctttgt ttttagtaaa gacaattttt tcattcttct tgtcgttatt aacattattc
     5101 ttcttcagta gacagtatcc atttgttcct atacaattat cactcattgg ggctgtaaca
     5161 gtgggtatac catcattctt cttagctctt gaagcaaaca aaagtagaat ttcaggaaat
     5221 tttttactca atatattaaa aaatgcttta ccaggagcac tatgtgtgac agtaaatgta
     5281 attattgttt acaatatagt tgaatatttg ggttatagtt caagtgtatt ttccacaatg
     5341 tgtgctatat taactggtgt atgtggtctt ttaatattaa gacaacaatg tttgccattt
     5401 aataaagaaa gattattttt aatagtaagt atgactattg catttgttat tgggattcta
     5461 tttttaggtg gattattctc atttgtagca ttgagtagag aattaattct cataactatg
     5521 gcattagcaa tattaatgcc aattatgata aaagttatgt tatttgtctt agatgagata
     5581 ttagagacaa ttgttccaga tttaaattaa ttttaaaatg atataaataa gagcagtttt
     5641 agttgaactg ctctttttag atgtgtttaa ttttatgtaa gaatatgtat ttttattaat
     5701 cagctatata tgagcaacag tagtcaacaa cttcattaac ctttaagtca aaacttgaat
     5761 cactaggaac attaaatttt tgtccttctt tgtaagttac aaactcattg cttccaggca
     5821 gttttacatc catacttcca gcaagcatct ccattatttc ttcttctcta gtagaaaaag
     5881 tgtattctcc tggcaacatt aatcctaaag tttttctttc tccattaggc agtattatta
     5941 ctctgctact taccttacca tcaaaataaa cgtttgcctt ctttactgca gtaacattct
     6001 taaattcact cattagataa aacctccaaa attatttgta catatatatt atataaaaat
     6061 aatgtaaaaa atgctatata tttacaaaaa aatttagtat ttattaaaaa aattaataag
     6121 aataaaaaag agatggaatt gcttaaattg ataaaaaata taaaataaat tgcaaagtaa
     6181 gcaagaaaag aataagttga agagttatat attataaata ttaaaaattt tagtattaat
     6241 aaaaaagata ttacattaat atgaaaatat aggagattag aagtgtttga taattacagt
     6301 acatgttaga tatatcattg taaattaaca tagctttgaa tataataatg ttcaaaaggc
     6361 aatatttttg aggtctgaat ccaataataa tatagtctta tgattgtaga tatcaaaaaa
     6421 tataagttac taagaatacg ttttgtagat ttatatatga ttaataacta aagctataaa
     6481 taaacaaaat aaaaagagta tgtttcaacc tatagctttt agatttaaaa tatactcttt
     6541 tattaattat agttgtataa attaaccata atatataatt aaaattttta ttataattat
     6601 atattaggtt cttcaattag ttttccacat acatatctat gaatgatatt tctatcatca
     6661 ccaacataga taaatctttc aagtctttca agtatagaat aattgtcatg atttaaattt
     6721 gaatcatcaa taatcaatgc atcaaaatca taaccttctt caaagctacc aacttttcca
     6781 aagaaacttc caccactttt ggttgcaata taaaaagtct cagataaaga taagaaatta
     6841 tacttcttac cagagttaac ccaatataat ttagataatt gtattgcaga taccattgct
     6901 ttaaatattg acaatgtatg tccaccactt atatctgatc caagtgctaa tctaatatta
     6961 ttatcgatta attttctgat aggcatagca ccactaccaa cattaaagtt agaagctgga
     7021 cagtgaactg ccataacatt attttctctc attaaatcta tttcatcctg agaacaatga
     7081 acacaatgag ccattaaagt aggagtttga ccaaataaat tatatctatt atatgcatct
     7141 ccatagaatt tagattcagg ttctagactt ctaacccatt caatctcatc ataattttca
     7201 gataagtgtg attgaatagg aatattatat tttaaacata aatctcctaa accttttaaa
     7261 agttcacttg tacagcttgg tataaatcta ggtgttatta ttggttttac aatagaatct
     7321 gtatcactat acttatttat aatttcttct gtatcagtaa tagaatcctg agtgttttct
     7381 aataaagtat cagggcaatt catattcata tttactttac caacgtatgc tccaagacca
     7441 acttctttga atatatccat aagtttcata gaggcttctt tatgtactgt tgcaaacact
     7501 gctattcttg tagttccaga tttccaaagt tcttttacaa attttttata catttttgtt
     7561 gcatattcta tatctttata atttgattct tctggaaatg tataagtttc aagccatggc
     7621 aaaagctctt tatccatagc cattccaaga tttttaaatt gagaggcatg ggcatggagg
     7681 tcattcatac caggtattat aagcttatca gtgtaatcta ctacttttag gtttttgtat
     7741 tccttaggta tctctttaaa tattcccttt actttacctt tttccagtat aatataacta
     7801 ttttcaaata ctgtgaaagt ttctgaagtt tttgtaaaaa ttatatttcc ttttaatact
     7861 tttaacatat aggtctcctt agttttaaat ttgttttaat atatgtaggt aaaattttgt
     7921 tttacaaatc aaaattttac cacatgtatt atatcaaatc taaactataa atactattag
     7981 ataatagcaa atattagtga aatacagttg tttactatta ttatatataa ctgacattta
     8041 agtgacattt aagaaaaata taatgcctac ttacataaaa tggaatgtta tttaaagaga
     8101 actttgatta tattttcaga agctttttta tccatatcgt ttaaaatatg agaatatcta
     8161 tttatagtta tttttatatt agtatgtcct aatctttcag agatgatttt tatattagtt
     8221 ctagctagaa gaagaactat tagaatagat aatatagtaa gtatttacaa atatgtaggt
     8281 gttcttaaat tgataaatta ttccatttta attttatagt ttgaatttta tgatataata
     8341 aaaatatata aattttgcag tgagcgatat ttttgataaa gtagggttta acagttgcaa
     8401 tgtaagggat tgagggtgta tgataaatgt tatcaattgc actactcatg gttcactgca
     8461 aatttgagag agttgtatgt gtgtaagtac tgaaaatact tagtttattt tggggtttta
     8521 tattaactaa gtggtatgta aatagaattt agtactttct aaaaatggat tgcctagcaa
     8581 gttttatatt aactaagtgg tatgtaaatt ttcctaattc cctatggtgt agcattggtt
     8641 gatagagttt tatattaact aagtggtatg taaatatatt aaagatatga cactctttta
     8701 tataaaatat aagttttata ttaactaagt ggtatgtaaa tacaagttca gtagggagac
     8761 agacagtaat tttaaaagag ttttatatta actaagtggt atgtaaattt attattatta
     8821 tatatcttga cttagcacta tttttgtttt atattaacta agtggtatgt aaatattact
     8881 ttgaaagcat taacagaaaa agagattagt aagttttata ttaactaagt ggtatgtaaa
     8941 ttatttactg ttgaacctac atcttgattg gataaaaagt tttatattaa ctaagtggta
     9001 tgtaaattaa ggttttaatt tattagtaat aaatataaga cctagtttta tattaactaa
     9061 gtggtatgta aataattctt tgactgcatc atttaaataa tctagctgag ttttatatta
     9121 actaagtggt atgtaaattc taaaaggtca catatattta ttaaatctgt tgccggtttt
     9181 atattaacta agtggtatgt aaatcatata atgaattaca taacatctta tgatattcat
     9241 cgttttatat taactaagtg gtatgtaaat ttcatagtct ttagagtctg taaatacttt
     9301 actcatagtt ttatattaac taagtggtat gtaaatttac tttaaaatat gtaccataaa
     9361 aaaatatatc aagttttata ttaactaagt ggtatgtaaa tatctctggc gtataacctt
     9421 cttttcctat tagttctggt tttatattaa ctaagtggta tgtaaatagg gtttctgata
     9481 aaatcttcaa acatgtaaaa tatgtgtttt atattaacta tgaggtatgt aaatcttata
     9541 cttagttaga actatatatc gacacaaata tgctttatat taactatatg gtatataaat
     9601 tgcaattttt attcgttgtc caatcgcttt gaaatttgtt ttatattaac taagtggtat
     9661 gtaaatttgc aacaagtata ggtaaaatac cccaataatt tatacagcat tttctccttt
     9721 aaaatataat tattttttat catttgtagt aaataattac cacataatat tgactttagt
     9781 tttaatgatt aaaatataaa agtagaataa ttataaaaag tattgaaaaa tttataaata
     9841 tatataataa aacttaatga caagatatta gatataaaaa ataattacct tataaataga
     9901 ttgaaattta tgaatattca tactataatt taaatataag gagatgccct ttgaaaataa
     9961 aaattaaaaa atatttaatg ctattacaat aggaactaga actacactta ataaatatac
    10021 agaaattaga tttggctcaa tataaaatac aaatatagaa tttaggtgtt ttttatgaaa
    10081 aaatttttat atgctttata tagtttcatt gttattatag ctaattttag attaaaagaa
    10141 aaaaattata actttatttt attagcaatc ttttttatac tgctgttaat tcaaaatgga
    10201 gttttaaaaa caggttatca aaatagagct acattttatc aagataatta ttttaatgga
    10261 gaggataaaa atgataagat aaaatttatg actataaaga tttttatatt tttgtcatta
    10321 ccattatgtt ttaatatact attcaattat ttaacttctt aaattatatt tagatatagg
    10381 agtcttatga taaaagtaag cttatgtgtt atttgtagta aatataaagt gcatatagtg
    10441 gtaaaacaaa agtttcatat aaatgaagaa aaaaatataa aataatttat ttaactactt
    10501 aaaaataaac taaaagggat aataaaatag cttttaggtt atggtaataa aggtatttaa
    10561 aaagagtgca taagcactcc aattgtgaga aatacataca caaatcatta ttacaacaat
    10621 gaaaaatgta catataagag acgttttgta tttgttttaa gtgataatat tttaatacta
    10681 actaaataat tatcacaata gtaaaaaaga aaaaggagta ttttgtactc catttgtcaa
    10741 aaatataaaa cttttatata agcttattat aacataattg ataaaagtgt aaagatatat
    10801 aatgaattta taaagaaaaa taaaataaaa aagataaatc ctaatcaaag gatttatctt
    10861 tttgtataga aaataattat tctattatta aatggcatgg ctaaactgga taataattta
    10921 ttattactgc tttcaatgtt ttttctcctt gattatggta tgtatgaatt gattcagctg
    10981 aaaaacaaat agagtcattt tctctaagtt gataaataga gtcatttact tctaatttta
    11041 attgaccttc tataatcata atatattcct gtgatttttc agaatgacca acagatgtat
    11101 aactatgacc ttcttctaat tccatttgaa ataattcgaa gtttctagtt ggtgtattgg
    11161 gataataaca ataaagacga taatctttat tttctgaaac ttgtacatct atatcagttt
    11221 ttgatacaat aaaagtttca ttttgaggtt gttccagtat tgcagtatat ggaacattta
    11281 gtccacttgc aattttccaa attgtgttta ctgttgggtt tgagtctccc ttttcaattt
    11341 gcgaaagcat tactttactt acgccagata attcagcaag ttgacctaga cttaagttac
    11401 gttcattacg taatctattt aaatttttag caataacagt attaatactc atagtagctc
    11461 cttaaaatgt aaattattga caaatataat ttacgaatgt ataatttatt taacattatt
    11521 tataatttac aaatgtataa tataatgaac gttaaataca ctgtacatcc gtaatataaa
    11581 ttatactgca taaagagttg tttttcaacg gatgatagtt gagagattaa gggggaattg
    11641 taatgcgtgt acttaataat gttacagagg ctataggcaa tactcatatg ttacatttat
    11701 ccaaaatagc agatgaattt ggagttcaag gtaatattta tgcaaagatg gaatacttaa
    11761 atccgggatt tagtaaaaag gaccgtgtag cattacaaat gattaccgaa gcagaaaatg
    11821 caggtttatt agttccaggt caagcagttg tggaattaac aagtggtaat actggaacag
    11881 gactagcaat agcttgtgca tgtaaaggat ataaatttat agcgtgtatg tcaaaaggaa
    11941 attctatgga aagagcaaga atgatgaaag ctttaggtgc tgaagtagtt cttgtagacc
    12001 aaatgcctaa ttcagttcat ggacaggtat ctggtgaaga tttggcactt gtagaaattg
    12061 aggctcaaaa aatagcaaaa gaaagaaatg catttagagc agaccaattt aatttagaag
    12121 cttgtaatca tgcacatgaa aagtatacag cagaagaaat gtgggaacaa ttggatggaa
    12181 atattgatgt atttgtagat tttcttggaa gtggtagtac ttttgcagga tgttcaaaaa
    12241 cacttaaaaa acataattct tctatcaaat gttatgttgt tgaacctgaa acagcagcaa
    12301 tttattcagg taaagaaata actgaccaag ggcataaaat acaaggtgga ggatattcta
    12361 tggatttgcc attagtagac aagaaactta ttgatggtta tattcaagtt acagacgaag
    12421 aggcaactga tgtagcaaga aaattagcta aattagaagg tgtatttgca ggattttctt
    12481 caggggcaaa tgtatgtgca gcaattaagt tgttaaagag cagtgaaaaa ggaaaaaata
    12541 tagttttaac tcttaatgat agtggtttaa aatatttaag tactgattta tatgaagaat
    12601 tgtaataata gcacaatagt tatgcaatgt tttttaaatt aagtatatag atttaaatta
    12661 taaaaagtat ctcaatgtga tttttgaaag ttataaagga gatacttttt tattttgtaa
    12721 atatggagta tcctacttat aagtattaag ctatatagat attgatgatt agctatgcaa
    12781 taaaaaagag tatttgtttt gcatttatac atcataactt ggaataatac atattaatgt
    12841 tctaaatgta tttaaaacat atgataaaat tagaacaaac agatacaagg agataaacat
    12901 gcaaaagaga attttaagaa attttatatt tacgggtaaa aatattttaa gtaaacttgt
    12961 ttttataata aaatataatt ttagtacatt aattttattt gaattatttc ataaaggatt
    13021 agccttattt tttatatggc catctattag atatatattt gatattttaa taaaaagttt
    13081 acatatagtc tacctaaata tggataattt tataaaagtt ttgaccaatc ccatgtcaat
    13141 agttctgata atactatcag ttataatact agctttttat gcattttttg aatttacctc
    13201 tgtaattatt tgttttaata aatcgataaa atatgaaaaa atagggttat ttgaactctt
    13261 taaaatatca tttgagaact ctataaaatt gttatatcct aaaaatatac tattatttat
    13321 atttgtgata ttaataattc cattagtaaa tacagtttta atttcagggt ttataggaaa
    13381 aatacaaata ccagagtaca tactagatta tataaagtct gatataattt taaatataat
    13441 ctatatgatt tttcttttaa taatatatat ttcggttatt agatggattt ttagcattca
    13501 tgagataact ttaaatacag aaaattttaa aagagcaaga aaagaaagtg ttaatcttat
    13561 aaagggaaag attataagaa caattattta ttcattaata ttatctttag tagtagctat
    13621 tattggatat gcgatttatc atagtggaat tatatttata ggcatatgga ctaaatattg
    13681 tactgatatg gaatttttga agaatgtatt tataaataga tgtattgcat ttgaagaata
    13741 ttttagattg attttgtcaa tattcatttt tatattggac attggattta tctcagcaac
    13801 ttattatcaa tataaaggag taagtatttc taaaaataat attaaaaaag agaaaaaatc
    13861 tatatcaaaa attgttttta atttagtatt aatattatta atttcttata tagagtcagt
    13921 tgcattttct tcacatataa atggtatgtt taatacatca ttttcatttt atacaacagc
    13981 aattgctcat agagcaggag ctgaaatggc tccagagaat actttggcag caatagaaga
    14041 agcttcacat gctaaagctg attatgcaga aatagatgtt caagaaacta aagatggaga
    14101 acttattgta cttcatgatt caaactttaa gagaactaca ggagtagata aaaatgtttg
    14161 ggatgtaaat tataatgaag taaagacata tgatgcggga agcttttatt attatggaaa
    14221 aggttattat gatgaaaagt ttgttgggga aaagatacca acactcaaag aaaagataaa
    14281 atattctaaa ggtaaggtga aattattaat agaaataaaa cttaatggac atgaaaaggg
    14341 agacgtagta aaaaaagttg ttcagttaat taaagaaaat aattttgaaa atcaatgtat
    14401 agttgcatct atggataaaa ctatattaca aaaagtaaaa aaactggatg agaatttact
    14461 gacatgctat tttatggcaa ttgcatatgg tgatttttca aagttaaatt atgttgatat
    14521 atatggaatt gaatcaactt ttgttaataa aacagtagta gaaaagattc ataaaatgaa
    14581 caaaaaaatc ttcgtttgga ctgttaatag tgacagttta attgagaaaa tgcttgacct
    14641 aaatgtagat agtattatta cagataatcc ataccttata gagagtgcaa tttattggaa
    14701 gaaaaatgga tttattagtc aagtgtcaga ttatttattt taaatattct ataattgtat
    14761 tatccagtaa aagagatatt attaaaatca ataatatctc tttttaacat aaataaggtt
    14821 gttaaattaa ggttattctt ataaaaattg tgcttatcag ctataaacaa ggaataaaag
    14881 tgaaaaaatt gctctgagat tattacagtt aaaagagttt taagatgaca gagagataga
    14941 ttgtcaactg tatatttaat aaatcttgtt atttattaaa ttttatataa gtaaaatata
    15001 taaattaata aatcttatgc tagaatatat atgaaaaaca atataaattt ggaggattaa
    15061 aaacatgaca gagcgagaaa aaatgttggc aggagaactt tacgattgtg gtgatattga
    15121 gttgttaact caatggcata aagcaaaaga cctagtaaga gattataacc aaacagattc
    15181 ctcggatttg gaaaaaaaag aaaaaatatt aaatgagtta cttggtggta agggtaaaaa
    15241 cttatggatt actgcaccat attatgtaga ttatggaaat aatatttatt ttggaaataa
    15301 ttgtgaagtc aatatgaatt gtacgttttt agatgataat aaaattatca ttggggataa
    15361 tgcactaatc gcaccaaacg tacaaatata tactgcattt catccaacta atgctggtga
    15421 tagatttggt aatgccaaag aagatggttc atttgaattt tgtaaaactc aaacagctcc
    15481 agttactatt ggagataatg tttggattgg cgggggtgct attatcatgc caggtgttac
    15541 tattggagat aatgtagtta taggtgcagg aagtattgtt acaaaagata ttccaagtaa
    15601 tatgattgca tacggaaatc catgccgtat tattcgagaa aataaataaa gttaaatttt
    15661 cttgcaaaat ctcaaaaaat acctttctta ctttatacat agggtatttt ctaatacttt
    15721 cacatccata ttcatttgtt aaatttttat ggaatgtagt ttttatcatc tctatcttac
    15781 attttcacta tttttttata aaagtcattc ctttatagat ttatttataa tagtatatat
    15841 tctatttgat gtttgaataa gcacttattt ttaagtaaaa tactaacttt taggttagta
    15901 ttttgctata gtatgatgat taagtaagaa taaaatacta taatacataa agcttaacaa
    15961 atataatttt tattttactt agatattttc ttttgattag caatcaaaag tggttataat
    16021 ttttttaatt aataagagta acatttattt tatgaaagag gattatatta atatatattt
    16081 aaataggaaa aattgtaaaa agcataaaaa taatataatt aattaatgag ataaaagtgg
    16141 ataaaaatat tgggagttta atttttgtga aattaaaaat attaaagcta atctacaaat
    16201 atagaatata ttctaagacg aatttttata attactaaat aaaataaatt aatcaatata
    16261 gttttttaat ataaacaaaa aataaaaaac ttatataaat aaaatgcttg tttttcttgt
    16321 attatttata taaatattga aatttgtcta gttatacgtt tttattgacc agttatgcag
    16381 ttgatattga atattctaaa tattggtgtt attttatagg tggtttaaac atattagggg
    16441 gattttatat atgatttgta tgatttatag agaatgtata aaatagtaaa ggtttagagc
    16501 catgtttagt ggattgcaga atatgcatat agaatgattc ttgtaaatta agttagacat
    16561 aggaaatata taatttgtat tggatgattg attttatggt gtatgctttt aacttctata
    16621 atgggaaagt aaagatgtac tcccaataca tctttacttt agcagaagtt atagttactt
    16681 ataaagtaca aaaaattata tatttattag agtagataaa tctaaaaagg tttttctaga
    16741 aaaatttagt tttaattgta tctacttact aataataaga cttgatgtaa gcaggtttca
    16801 actaccgttt ctccatattc tgatataaaa tcatctaact gtgtatctaa aaaattacaa
    16861 taatcatatg aactactgat gtaagcagtt agtatatgtg tacatttgtc tatttctcca
    16921 ataaacatat tatccatccc cctaaaaaat ttagtaaaaa taaaaagtcg cattctatta
    16981 gtcaattaac ataatttata aaatttttta gtactagttt ctataactta actccttaag
    17041 gttaaatatt cccagtttta ccaatttagt tttattctac aagtatataa tactagaaga
    17101 tgctccatta ttcaacatgc ttgtttgaga atagaaaata ttctaaaatt ataattgttt
    17161 ttaaaaataa gattcaagat aaataaagtt atatgcaact atttaataat ctttccataa
    17221 taaggacagt aggttctatt cttaaaaaa
//
LOCUS       DAGSRM010000084        17188 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.084, whole genome shotgun sequence.
ACCESSION   DAGSRM010000084 DAGSRM010000000
VERSION     DAGSRM010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17188
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.084"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(122..646)
                     /locus_tag="MJE33_002844"
     CDS             complement(122..646)
                     /locus_tag="MJE33_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBY9648768.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQVMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(711..1463)
                     /locus_tag="MJE33_002845"
     CDS             complement(711..1463)
                     /locus_tag="MJE33_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBY9648769.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(1494..2792)
                     /locus_tag="MJE33_002846"
     CDS             complement(1494..2792)
                     /locus_tag="MJE33_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HBY9648770.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEGIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMEDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(2809..5073)
                     /locus_tag="MJE33_002847"
     CDS             complement(2809..5073)
                     /locus_tag="MJE33_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HBY9648771.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5328..6146)
                     /locus_tag="MJE33_002848"
     CDS             complement(5328..6146)
                     /locus_tag="MJE33_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBY9648772.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6139..6837)
                     /gene="cbiQ"
                     /locus_tag="MJE33_002849"
     CDS             complement(6139..6837)
                     /gene="cbiQ"
                     /locus_tag="MJE33_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HBY9648773.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEFFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(6840..7118)
                     /locus_tag="MJE33_002850"
     CDS             complement(6840..7118)
                     /locus_tag="MJE33_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HBY9648774.1"
                     /translation="MSAKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEITK
                     INPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(7108..7860)
                     /locus_tag="MJE33_002851"
     CDS             complement(7108..7860)
                     /locus_tag="MJE33_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HBY9648775.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAIPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8042..8234)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8366..10138)
                     /locus_tag="MJE33_002852"
     CDS             complement(8366..10138)
                     /locus_tag="MJE33_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HBY9648776.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDIILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(10152..11081)
                     /locus_tag="MJE33_002853"
     CDS             complement(10152..11081)
                     /locus_tag="MJE33_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBY9648777.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            11309..11497
                     /locus_tag="MJE33_002854"
     CDS             11309..11497
                     /locus_tag="MJE33_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY9648778.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRTKRRKEVQYE"
     gene            11490..12191
                     /locus_tag="MJE33_002855"
     CDS             11490..12191
                     /locus_tag="MJE33_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY9648779.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAIIAHCGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGQVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            12193..13116
                     /locus_tag="MJE33_002856"
     CDS             12193..13116
                     /locus_tag="MJE33_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY9648780.1"
                     /translation="MNTSVYLLLILVLSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            13273..14196
                     /locus_tag="MJE33_002857"
     CDS             13273..14196
                     /locus_tag="MJE33_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9648781.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKEREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            14189..14926
                     /locus_tag="MJE33_002858"
     CDS             14189..14926
                     /locus_tag="MJE33_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9648782.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPIKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANIALGRRK"
     gene            14937..15710
                     /locus_tag="MJE33_002859"
     CDS             14937..15710
                     /locus_tag="MJE33_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9648783.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETNLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            complement(16237..16845)
                     /locus_tag="MJE33_002860"
     CDS             complement(16237..16845)
                     /locus_tag="MJE33_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBY9648784.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
     gene            complement(17023..17139)
                     /gene="rrf"
                     /locus_tag="MJE33_002861"
     rRNA            complement(17023..17139)
                     /gene="rrf"
                     /locus_tag="MJE33_002861"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         5530 a         2985 c         2172 g         6501 t
ORIGIN      
        1 ataaattaaa aagtgaaatg tatttgtcta aaaacataat aacatttcac tttatcctca
       61 tttttaagta atatatattt ttaattttta tttataacta aatctagttt ttatataaat
      121 tttagttttt ttctacatct gagtaatcta ttccaaaagt ttctacagta acagatttca
      181 taacttgtgg agtttgtggc ttatcagctg aatctgttct aactgatgct atttcatcaa
      241 ctgtatccat accttctatc actctaccaa aacctgcata ctgaccatct aagtgtggtg
      301 agtttttatg cattatgaaa aattgagaac cagctgaatt aggtgccata gttctagcca
      361 ttgatatgac tcctctttca tgttttaaat tgttagtaaa tccattacca ctaaattcac
      421 cttttattga gtgacctgga ccacccattc ctgtaccatt tgggcatcct ccttgtatca
      481 taaatcctgg tattactcta tgaaatatta ttccattgta atatccttca tttactaatg
      541 atacaaagtt tttaactgta tttggagcta tatttggata taactcaatt ttgatttctt
      601 ttccattttc catttctata gttactatag gatttttatt ttccataatt tattaccttc
      661 cttttcatta gttaaattac ataattacat ttattatatt atactctaaa ttaaagtcta
      721 tcaagtatat tttttcctat ttcatatcct tcttgataaa tagttttaca aaactcatca
      781 tcaaagttaa gcgtgtcttt aggagcaaat tgagtattag gtctaactac tattatttta
      841 tcttcatcat atactttttt aatatcttct ggcaactcat aattatattt tgtggatatt
      901 aaaataattt tatccacatt atccccaatt aatggttcaa ttaatgcact cctaattaaa
      961 cctccatcta atttgaaacc atcataaatt ccttcttgca catctttcac aaattgttct
     1021 ttaaatccaa gagttgcgtt tggatttgat ggtaacaacc cactgtatga aatgctgata
     1081 atggcttcat tacgaggcat ttttgatacg tcaacaatct tttctgatac tactttatta
     1141 tcaacttcaa cataattaac ataaaagttc atttcttcta tattactatc atctagttcc
     1201 cttaggttgt ctatagcagg agaattatcc acagtattat ttactatttt tacatttcca
     1261 ttctggatat cttttatatt tgtccacatt ttctccaagt tatcaacatt ccctgtgtac
     1321 aaataatatc catttattgc tcctattgat gtccctgaaa tcgaataaaa tttatttatt
     1381 cctccatcat acaaggcttt aactactcct gcttgataag ccccttttgc tcctccacct
     1441 tctaaacata atcctaattt catagtctca tctccaaatt gtttatctta tatttatatt
     1501 tcaaaaaact tactaaatgt tttatatata ctatagtggt cctcaacatt tcctaattct
     1561 cttatagaat gcattgctaa aattggactt cccacatcca cagatggtat atctatatga
     1621 gtagaagata ttggacctat agtacttcct cctctttcat ctgacctatt aacaaattct
     1681 tgatactcaa cattagcttc tctacatata tttttataaa tagctataga atatccatca
     1741 ctagtatatg cctgactagc atttatcttt ataaccggac cttttcccat tattggttta
     1801 ttagttgggt catgcttttc tggaatgttt ggatgaatag catgagctaa atctgatgat
     1861 atcataaaag atgaataaat agactcaaag aatccctctc tatccttacc cattgatatg
     1921 caaattctct ccaatatatt taacaacatg tttgaatctg caccttgttt agtagagcta
     1981 ccaacttctt cattgtcaaa tacagccatt acatttacac catgttgacc tttagtatta
     2041 acaagagcat tcaaactagc atgtaccatt gcaagattat cttgtctccc aatagatata
     2101 aattcatcat tagcccctat taaacttcct ttttcaaatt catataaaaa taaatcaaaa
     2161 tctataatat cttccatatt tacatctaag ttagatgcta tttgctttat aagaaaatta
     2221 tctttttcta atgtatcatt taacatccca actaaaggta acatatcttt ttgcttatta
     2281 tatgaatgac catcatttat acttctattt agatgtattg ctagatttgg tattatacaa
     2341 attggctttt taaagtctat aagcctttca tttggtttta atatactatc accttttaaa
     2401 actactctac cagctatccc aagaggtcta tccaaccatg tacttaatat tgctcctcca
     2461 tagcattctg tatttaactt caaataagat ttttcagctt ctatttctgc atttggttta
     2521 atactaaatg taggagaatc tgaatgagag cctattattc taaatccatc tctttctata
     2581 ccttctgaat ttactacaaa agctactata gctgataaat tcttagtaac ataatacttt
     2641 ccaccaactc ttagattcca tctttgattc aaaactaatt cttcaaatcc atttttatct
     2701 aagatttcct tagtactctc gactgcatga aaagacgacg gactatcata tataaagtct
     2761 attaagtttt tagcaaagtt tatactatcc attcctcatt tacctccatt ataaaatatt
     2821 tagtgttgta tattctttat ttattttttt aattccctgt tggtcaaaag ctatagtaac
     2881 atctgtacca attatagaaa ccactgtccc aaccccaaat ttaggatgat gtaccttaga
     2941 accaagtttt atatcatctg gattagtttc tttattagaa tcttttattg tagcattaac
     3001 tttatctgca actttagatt tgcttatagt actcatatac tttttagtat atttatctaa
     3061 catattataa cttgctttag aatagcttag ttcttttttt actttgtaca atctttctat
     3121 acattcttct ggcagttctt ccataaatct tgaagctatc gcaacatttg ttttaccata
     3181 taatgttctc ttctctgtca atgacatata tagctcttct ttagctctag ttataccaac
     3241 ataacaaagt cttctctctt cttctatttg tgaatcactc atagatttta tagctcttga
     3301 tataggaaat aacccttctt ccatacctgt taaaaacaca acagggaact ctaaaccttt
     3361 ggatgtatga attgtcataa gcgaaaccct atcattgtct tcttcatcat ttgattcaga
     3421 tgtaagtgct atacttgtta ggaatgtctc taagtcttta ttttcataag tatcatcatt
     3481 actactttca aactccaaag ctatagaaat aaattctctc aaattatcaa ttctgtcttg
     3541 agcttcttca cctttgcctg ttaaatcttt ttcattttta tttcttatct ctacaagctc
     3601 gtccatatat cctgtagtat ccaagacttt ttctataagt ttgctaacag gataaacttc
     3661 ttttatagtt ctaagagtcc ctataatatc aacaaatcca cttatacttg cccttgcctt
     3721 agtagatata tctgaatttg tttctatatc aattaatact gagtatatac tttcctgttt
     3781 taagttagct ctatcttcaa ttttttctat agttctaagc cctatacctc ttctaggaac
     3841 atttataatc ctttttatag atatatcatc ttgtggattt tgaataactc ttaaatacgc
     3901 caacaaatcc tttatttctt ttctctcata gaattttgta ccaccatata tattgtatgg
     3961 aatttgactt ctatttaaag cgtcctctac aggtcttgcc tgtgcatttg ctctataaag
     4021 tactgcaaaa tccttataag gtctattttc ttttctagct atttgtgcaa ttgaatcagc
     4081 aatgaaatca gcctcttcta tttcgctacc agttaattgt atctttataa gttctccttc
     4141 ttttttttca ctccaaagct tttttctttt tctttctata ttattagata taactttatt
     4201 agctgcatct aatatcactt gtgtagacct ataattttgt tctaacttta caacatgtac
     4261 attgtcataa tctttctcaa attcaagtat gtttcttata tctgcacctc tccatccata
     4321 aatactttgg tcatcatctc caacaacaca aatattttga tgttctcttg ctaataattt
     4381 tatcaattca tattgagctt tacttgtatc ttgatactca tctaccatta tgtatctaaa
     4441 tctacttctg taatatgcta atacttcatc attagccttg aaaagttcta cagttttaag
     4501 tattaaatca tcaaagtcaa gtgcactatt tctttttaaa cggtcttgat atagcgcata
     4561 aatatctgca atcttaacca ttctattatc tgccatattg atatctttaa actgctttgg
     4621 agtataaagt ttgtcttttg ctcctgaaat tgcacttata actgcttttg gttcaaacac
     4681 tttatcactt aaatttaatt cttttagaca gtctttaacc aatgtaactt ggtctgctga
     4741 atcataaatt acaaaacttc tattgtaacc aatcttattt atatcttttc ttagtatacg
     4801 aacacagcaa gaatggaatg tacttatcca catatcctta gtttcaggac ctatattttg
     4861 ttcaactctc tctctcattt catttgcagc tttgtttgta aatgttatag ctaatatatt
     4921 tggtgcttgt acacctttat cttctataag atatgctatt cttgttgtta ataccttagt
     4981 tttacctgaa ccagcaccag ccagtataag tactggacct tcagtctttt ctactgcttc
     5041 tctttgagca ggatttaatg tatctaaatt catatttctc ctcctgttaa cagccataaa
     5101 tatattttaa tctaaaatat gaattacttc aaattataat tatgtattat attaatagat
     5161 tatttccaat atattacaat taaataacat ttttccatat aatttatttc attgtttaat
     5221 aaaaaaaata accttatgat ttggggaatc ataaggcacc tttaaggggt ctaggttaag
     5281 ggttattata tataaatcta tttttttaaa ataggtttag ggcttaatta tttatttgta
     5341 ttaagttctc tttccttcca atacttatac aaatcttctt ctttcttaaa taagggtaaa
     5401 ttcatatttc tatgtacttt tactaaccat ggggattcta ggccagcttg tataatatta
     5461 ttttcattta taaacacatt ttctgctttt ccttcatcaa tcaaaatccc cttatccaat
     5521 acatatatgt aatcacatat ttcatacatt aaattcatat catgactgga tattactatt
     5581 tttatattat ttttatttaa tccctttatt atatctacaa tagacctagt acttactggg
     5641 tctaaaccag ctgtaggttc atcaagtaat acaatttcat tttccattgc tataactgat
     5701 gctattgcca ctcttttttt ctgtccatag ctaagaaaat gtactggcct gtctataaaa
     5761 tctattccat taacagctac tagagctttg tttattctct ctttaattgt tttttcatcc
     5821 ataccaatgt ttctcattgc aaaggctata tcatcatata cttttgaata aaaaatttgt
     5881 ttttctgggt cttggaagac aatacctaca ttttttctaa ggttgtataa acttctctta
     5941 tcatatttta acttttcttc tttaaaaaga atttctccag atgtaggctt taatataccc
     6001 attaaattca taaataatgt tgattttcct gaaccatttg aacctattat acctataaca
     6061 tttccctttg aaaaatccat attaatgttt aatagtgcat tggtattttt ttcatattga
     6121 tacgttaaat tatttatttt aaacatcatt atcccctact atgtgaaatt taccatcata
     6181 caattttatg tctaatgata tacacatatc atcatacctt gtcataagtc ttttgtacag
     6241 catacttcca agaataccta aggatttata tgaagttttt aaatttatat atccaaatct
     6301 tagctcttgt gacttgtata tttctgaaaa ttcttctaaa aatatgaata taaacctata
     6361 tattaacatt gataactcga caaaagtgtc aggtaaatga agttttttaa aaaagaatat
     6421 taattgatta aaaggtgttg taagtataca aaaatacaca caagtcaaac aagacatcgc
     6481 tctaaataac acatgaatag acatgtcaat agacgccttt gatattccta cattaaaact
     6541 aaaaaattca aaactatata ataaatctgc tttattaaaa gatatattta caagagttat
     6601 tcctatacta agaaataaaa agtacattgg tattctaagt aattttaaat aattttttaa
     6661 atctattcca gccacacaaa caaccaaaat actcattaaa atcataattc cacttaaaat
     6721 aaatatattt tgtattacca tagaagcaat cagaaaaatt actcctatgg ctactttttt
     6781 gtttggattt actttactca atctatttgt atatgcatat ttatcaatta taagcatgat
     6841 taacttctct ttcctttttg aagacctaat atgtatccta ttacaccagc ccctaaagct
     6901 gcttgtgcag agaataataa actttctatt tctccacttg gtggctcata tggactgcta
     6961 aaccatggtt tgtaatttgg atttatctta gtaatttcac tttcagcttg accatctgct
     7021 cctccatatt cagcacctga gttgacaagt aatgggaata taataagagc tactactaat
     7081 aaaagtaata atatgttagt ttttgtttta gcactcatta gctttaactc ctttatcatt
     7141 attttctagt atgctttttt ctccgttttc tgtaattaaa ttatatacaa ttacagtaag
     7201 tataccttct gctattgcta ttggtatttg agtcataaag aaaattccta aaaatttaat
     7261 tgctgaagcc attaccccac ctgatgggtc tgggaaagcc agtgctaatt gtattgaagt
     7321 tattgtatat gtggctaagt ctcctattgc tgctgcaaga aatactggaa tagcattatt
     7381 tccatctttt ttctttaaag ccttaaatat taggaatgat acgattggcc ctattatcgc
     7441 cattgaaaaa gcattagctc ctaaagtagt tattcctcca tgtgctaata acaatgcttg
     7501 gaatattaaa actatagttc caagaacaaa cattacgctt ggtccaaaca ttattgctcc
     7561 aagacctaca ccagtaggat gtgaacaact tcctgttact gaaggtattt ttagtgctga
     7621 taatacaaac acaaatgctc cacataaagc taataatact ttctttttag ggtcttgctt
     7681 tactatttta cctattgatt ttaatccgaa taagaaaaat ggtataaata taactcccca
     7741 tgctatagac cattttactg gtaagtaacc ttccattata tgcattgcat ttgatgtaac
     7801 tggagttaaa atgattaaca taagtgcagc tataacccct aatttaatat tctgtttcat
     7861 cttagtatcc ctctctccct ttctaataat taaaacataa taatacatgt ctgatttatt
     7921 taataaaata aaaaagccta actcgatggt taggcttcta attttccaca atctaaatac
     7981 aaacacataa aacaaacata tctgtataat tattataaga aactttctcc atccccgaag
     8041 aaattacttt aatattttag gcaggtctcc tgacttagct tcatcctact cctcttcctt
     8101 cccatgagat ataaaaaata tcatcatagt ggctaacaaa agtttcgtct gcatcacagt
     8161 agcgggggct gtaaaggaat ttaacctttt tccctattaa tcttgcttaa agaacctata
     8221 atattatata tttaattttt aaattcttct taaattattt tctatataaa tcattgtata
     8281 ccttttctgt gaaaatttct acatttttta taaattttat tcatttttat atttttatgt
     8341 atataaattc ttttttgtaa gtttattaat ttctaattat cttctttaaa tacattgaaa
     8401 ctacatcaga aaatctagtt atatctcttg aatatataaa atcatttcca tatattaatc
     8461 tttcagcttc aagttctgat tcttttcctg taaatactcc taatactaat atattttgct
     8521 ttctagcttt tctcacttca ttggctgtat ctctcaaacc aactataccc ctataagcct
     8581 tttctcctct taatgttctc tcactacttt ttcctatctt tacatcattt ggtcttccat
     8641 cgctgagaac tatcaggatt ttattttctt catctctatt tattagacca ctacatatag
     8701 attttattgc caaaccatct ctattattac cagcacaaaa atattcaaat atattttcac
     8761 tattttttat actatcatca taatctttaa atctttttaa tattgtatag tctaaaaaac
     8821 tagaaaatcc catcactcta tttggtatgc caacttctgt tagagcattt gcaattatgt
     8881 aagcttgaat tgccacattt ccctgatttc tactttgaga gccacttgca tccaataata
     8941 tatcaataac gtatttccct ttttcatttt ctatatcttt ataaaatatt ttattattat
     9001 tacttctacc aactctccaa gctctatttg ctaatatagt accacaatca gaatatactc
     9061 tacttttagc attttcttct attaaaattc ttgatagact ttcttttaat ctctttattt
     9121 gctgtctata cattctagca tgttctctaa attttcctaa attattttct ttttgtcttg
     9181 ttacatattt tagttgaaat acattattac actcacttct aagaactcca tctgtaaagt
     9241 gtattctaca accttcatga acatttctac aattttgtgt ctcaattttt ttaatttcac
     9301 ctttactcaa ataagttttt ccataataat gttctatttt tgaatatatc ttatttgcag
     9361 tgtcttcatc aacatatata actcggtctg ctgaattgct acatttagaa tcatcaaaat
     9421 cccctacacc ttctacaagc atggatgaag atattgaatc tatatcactt tcaataatat
     9481 tttcttcttc atcactataa agttcttcat acatgaaatc agcaaatgta tcaaaattta
     9541 cttctacact ttctacatta ttttcatcaa taagactatt ttcttctcca tttaatatgt
     9601 attcaaaata agttaaatat ataacatcta atgcttttaa aatctccata atatcttgat
     9661 tggtatctat atttcttata tcattaacta ttcttctagt catactatct aatgctgggt
     9721 gcttgtccat actataaagt acaaatgtgt atttaatttt atccataata ttatcattgt
     9781 gtattttggt aaaacgtgaa acaatatctt tataagcatt tcttcttatg tcttctatcc
     9841 ctggcctatc tttaatgacc ttttcttcaa ctattgtatt taatataact tgaattagac
     9901 ccagtataat atctttgttg tatccttttc ttattctatt tgtaagatat tttttaacta
     9961 tatcccaatc tacatacttt cttctagcac ctgcaattat tgcaaaatat agacctattt
    10021 cttttgaaga gtaatctttt tctgatatat ctatactttc attgtaatca ccagctattg
    10081 tccatgataa gttattagct ctattttcaa attcataatc atcatatatc catttcataa
    10141 tctcacctat attaaatatg acttattgga aacacatcta tactctccca tttattaggt
    10201 attctagttt taattacatc tccaaccatt tctttctcat atacatcaaa agtctttcct
    10261 gtaagtccca tattaattgc aagagttggt tttaatcctc ttcttatagt cttaagagat
    10321 gccataagac cacgtaaatc aatagcttta ctagatatct ctccattttg agacttcaat
    10381 tgtaaatcta aaaatattcc tgcaaagtgt attagttttt cttcatcagc atctgaaaat
    10441 tcatttttta aaatcatcat taacttatct tcttctatag gaggaatatc tattaccata
    10501 aatctagata ccagtgcttc attaagttct tttgttcctg catattcata attcatagtc
    10561 ccaataaatc ttgttgctgg atgtagattg actctctcat acccaggtac atcaattatt
    10621 cttctatagt ctaaagctga atgtaccaca actatagcat cattttttgc catattaatt
    10681 tcatcaaaca ccccaaaacc tccattaata gcacactcat aaactgagcc tcttctaagt
    10741 ttaacttcat tgtctataaa tgtatctgta cctataagtg ttgaactgtc tgtatttata
    10801 tgaaatgatg tattccactg aggtctatta aatatttccc ctagattatc agcaagtaag
    10861 ttttttcccg ttgcctttgg tcccgataat aatatatttt cttcttctaa aatagctgct
    10921 atacacatag acaagatatc tttaccataa aaataagttt tagactttgt tactctgtat
    10981 tctaaatctt tttctaatcc ataataattt ctaaaataca gtacatcttc aataagtttt
    11041 tcatctaccc cttgcaccct tagattatct attatatcca tatttttacc ccttttatat
    11101 aaatagtatt tgtttcattt cttatcacca ttttagcata aaaggatata agctaaaatt
    11161 gattttttaa tctccccatt ttaagtaaaa ccatgcacca ttttatcact acaaaattaa
    11221 gagtactact tactgatatt tcttgatttt gttcgtctat acgtatataa tatatgctga
    11281 taaattatga ggaaaggatg attgaaatat ggaatatatg tcttgcccag aagtagcaaa
    11341 aaaatggggt atttctgaaa gaagagtaca aaaactatgt gaagaaagtc gcataccagg
    11401 agtttcaaaa ctcgggtata tgtggctgat accaaagaac gcagaaaagc ctgttgatgg
    11461 tagaacaaaa agaagaaagg aagtacaata tgaatagcgt tttgattata gatgatgaca
    11521 aagaactttg tgctcttatg aaaaaatgtg ttgagcaaga aaatttatct gctattattg
    11581 cacattgtgg tgcagaaggt ctacatttgg tcgataaaaa tagacacaca ctctctcttg
    11641 taattttgga cataatgatg ccagatatag acggttttca agttctgaaa aaaattagag
    11701 aaacaagtaa tattccagta attatgctta ctgctaaaaa tgacgaggat gataaagttt
    11761 ccggactacg tttaggtgct gacgattacc taacaaaacc gttcagcata aacgaactta
    11821 tggctcgtgt caattctctt attcgtcggt atactatgtt aaaccctgct tttataacag
    11881 acacagattg cataataata aatggcatga ttattgacaa gacaaaccga attgtttctg
    11941 tcaataatat tcctgttcaa cttacaagca aagaatttga tttacttgct tttctggctt
    12001 ctaataaggg acaggtgttt accaaaaaac agatttatac acaagtatgg gaagaagaat
    12061 atgcttttga cgatagtaac atcatgtctt tcatcagtaa attgagaaaa aagattgaac
    12121 cagaccccaa ccatccattt tatattttga cagttcgtgg tgtcggttat cgttttaata
    12181 aggaggctta acatgaatac aagtgtctat ctgcttttga tattggttct tagcctgatt
    12241 attatagcgt accttgtttc tgtcctgcta cgtgtccgaa tacaacttac acttataaaa
    12301 gatgctttag aggatataaa aaatggaaac cttaatcgac gtattttaac agatgaaaac
    12361 gacattacaa aacaaatttg ctatgatatt aacgaaatag caatcaatag ccaatttcaa
    12421 cttattcagc aaaaacaatc agaacaagcc tacaaacagc taatgacaag tctctcccat
    12481 gatgtaaaaa ctccacttgc ttctttggtc ggttatttgg aagcagtaga aagtaaaatt
    12541 gttgtaggag aggaaaaaga cgagtatatc catgttgcgt ctaataaggc tcattactta
    12601 aagaattttg tagaaaatct atttgaatgg gtaaaattgg attctaagga acaagttttt
    12661 cattttgata tttttgactt gaatgaatta tcccgtaata ttatatctga ttggatacct
    12721 gttttagaaa gcagtcattt tgaatatgaa tttgatatac cagaaataga gtacttctta
    12781 cgtatagacg caaatgcata tacccgtatc attaataacc tattacaaaa tattattacc
    12841 catagtagtg gcaacaaaat gacattgaga atttttgaaa acaaagagca agctcaaatt
    12901 ataataacag ataatgggaa aggtatatct tcagacaatc tcccacatat ttttgaaagg
    12961 ttgtaccaat gcgaccattc acgagcttca aagggaaatg gtttaggctt ggcgattgca
    13021 aaggaactta ttaacgctca taagggaaat attaccgcca acagcactcc cggtatggga
    13081 acagaattta caatattgct tcctaaagcc ctgtaaaagt acgaggcttt cttactctaa
    13141 aacaagataa aagcaagatt atggcaaggt tttggcaagg ttcatgtttt ataattctaa
    13201 ttgtagttga ttttgacgtt gtatcaacta tcttttgtag aaacaaattt attaaaagtg
    13261 aggaaattat atatgaatga ttttgtgatt gaaacaaagc aacttacaaa aatttatggg
    13321 aatcaaacag tagttgacaa agtcaatctc catgtaaaaa aaggccgaat atatggtctg
    13381 ttaggacgca acggagctgg gaaaaccaca attatgaaaa tgattttagg attgacttca
    13441 atcacgtatg gcgaggtaga tgtatttgga gagaatatta aggaacgtga aaaacaagta
    13501 tatccccgta tcggtgcaat tatcgaaacg ccaggatttt atcctaatct gacaggaacg
    13561 gagaacttgg agatttttgc aaaactgcgt ggtacagccg cccctaattc tgtaaaaaga
    13621 gcattggaaa ttgtaggctt accttataaa aataaaaagc tgtttagtaa atattcactt
    13681 ggcatgaaac agcgacttgg tattgccaac gctatcctgc atgacccaga actgttagtt
    13741 ttagatgagc caaccaatgg tcttgaccct attggtattg cggagatgag aaagtttatt
    13801 agggacttga gcgttgagca tggaaagaca attcttattt ccagtcatat tctttcagaa
    13861 atctcattgt tggctgatga tattggaatt atcgaccacg gtgttctttt ggaagaaagt
    13921 agtatgagtg aactagaaaa gaaaaacagt aaatatatcc tgctacaagt ttcagatatt
    13981 ccgaagacat cacttatttt agagcgtcaa tttaacacga aggattattc cgttcaagat
    14041 gactacacgt tacaccttta taacactaat ctggatatgg gtgaaatcaa taaagcactt
    14101 gtaatgcaga atgtaactgt tattagttct caactttgta atgatacttt agaggactac
    14161 tttaaaaaaa tcacaggagg agagggcatt gcttaaactt attcaaacag aatttcttaa
    14221 actgcgtcgt aggaaactta tttggctgat gttgttgtct gctctcatta tgccgttttt
    14281 cgcactacta tacttcaatt attttggtaa aacaggtgtt gagccaataa agttttataa
    14341 atggtctgca tttggttata cactttggat tattttgcct gttgttttgg gtatattttg
    14401 caccatgctt atacacgaag aaaaccagta tgatatgctc aaacagcttt ggattgtacc
    14461 tattagtaaa atgggatact tttttagtaa atttttcgtt gttttgattt actctatttg
    14521 ttttatgctg attacagccg ttgcttctgt gttatttagt gtactttctg gatatgtcgt
    14581 atttagctgg gcaagcattt tatatttgtt gaaaaaatgt ttagaaattg gtgtaatcac
    14641 tgcttttgta atgttgccga ttttggcaat cgctacatct caaaaaggat acattctccc
    14701 aatttgtatc acattggtat atgccttttc cggttttgtt ataatgacaa taaatatgta
    14761 tttacaccca ttatccagta tgggagttat tatcatgaga aataaagaca ttccaggtct
    14821 tatattttca caggaaatta atgtcccatt agcatttctt tgtatttgta tttggagtat
    14881 tgtatcagta ttgtttgcaa atattgcact aggaagaaga aagtgaggtg tagataatgt
    14941 ggaaattact ttgggctgaa tgtcaaaaaa ttcgtcgttc aaaaattgtt tggattacag
    15001 tttttgcatc cattatgata gcagtcatag tttttgcagg ggggcaggat gtatataatg
    15061 acccagattt acattatggc ttaaagagcg tttatgacgg ttcacggtat atagacaatg
    15121 caggttggta tatggacgca gtacaacctt ggtcaacttt ttttgttctt cctgctgtca
    15181 ttgccctatt aggaagctat attatttgtc gtgaagaaga ggaggacact attaaatccc
    15241 tgcgtcttat tcccataaat gaaacaaatt taaccatagc aaaaatgatt attactttta
    15301 tattcagtat acttctttat ttgttactct ttactataac atttttgact gaatccgttt
    15361 tacatttttc cgatttatcg acaaaattgg ttttaagttg catgaaagag tattttcttg
    15421 atggcatagg tgtattcttt gcaatttctc ccattattgc cttggtatcg cgcatgaaaa
    15481 aaggatattg gcttgcatta gtattcacag aagtttattc tattgctgga ctatttgcgg
    15541 gtatgtcaaa tgtcttacaa accttttttc ctattgcggc aatatttaat atttctggat
    15601 actatataac tactggagaa aaaattatgg ggagtatcat aagtttatta ctttgtgcct
    15661 gcctttctgc atttatactt aaagggctaa aacacaatga aaaaaattga aataggtata
    15721 tcaaaatgaa aaagataaaa aaactgatta ttctaattgt attcttgttt ctttttggca
    15781 tgttacactg ttatactgaa tggaaattca acaataaccc acctaaaggg gtaatagaaa
    15841 gaataaatga gtggactgga ttagtaactt atgttaatta tagtgaatgt aatattagca
    15901 agttattgtt tcgtatttta atactataac tttaaatatt tggttttaat cttccaaacg
    15961 aatataaaaa aactgtcaca gaggcaacat atttttgaat atttataaag tggtagtttt
    16021 gattttcaaa gctaccgcct tttcttctat ataagcaaac ttatcctcaa caaagtgtaa
    16081 aacatggtac attttaagaa aaaagtacca cagacacaag caataaaaga aacgccgaga
    16141 acctacaaaa acgtagtgct tatgcagatt tacgacgcct tctattttct caaccgtctt
    16201 aatgaccttg tacaaaagaa atttttctaa tattttttat aatcttatct tgtgatgttt
    16261 tttatgtata aatgacttaa taacaaatcc aataaaaatt atggatattg gtataattag
    16321 aacgaggaat ttataatcat tataatgtga cattattctt acccaactct cagaaaaaat
    16381 atatcctata ctgattaatg aagtattcca tatggttatt cccacgatag atgatattac
    16441 aaaagttata atattttgcc ctgctatacc tgctataaaa gatatgtatg ttctacataa
    16501 agggattaat ctacatacac aaacagaaat agaagagtat ttattaaaat aatcttgaga
    16561 tgcaaatatt cctttctttg cctttggaca tatctctatt attttattta tgatagattt
    16621 acctccccaa tttcccactg tataacaaaa tatcgaacca agtatacctg caattatact
    16681 taaaattaaa attgtcagaa atccaaaatg atttcttgca gcaattgctc cacataaagg
    16741 taaaacaact tcgctaggta atggaaaaca agcatactca atcataatta atacaaatac
    16801 agagattaat ccatattgta aaataaaact attgattaca gacacttact tatctatctc
    16861 ctttcttatt taaaggtttt aagggtacat gttctaagga ttctcaccct atatttaaag
    16921 aatcaatctc tttaaactat tatatgtatt tacacaaagc atttataaca tactttaaaa
    16981 atagtacctt aaaataagaa acttaataaa taaaaaaaga ttacgtggct acgtcctact
    17041 ctcccaggca gtttcccacc aagtaccatc agcgctagag agcttaactt ctgtgttcgg
    17101 aatgggaaca ggtgtatcct ctctgctatt gtaaccacat aatctttatg cttaatattt
    17161 ccattaagtt tttaataagt ttatagaa
//
LOCUS       DAGSRM010000085        16853 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.085, whole genome shotgun sequence.
ACCESSION   DAGSRM010000085 DAGSRM010000000
VERSION     DAGSRM010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16853
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.085"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            323..781
                     /locus_tag="MJE33_002862"
     CDS             323..781
                     /locus_tag="MJE33_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY9648785.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFKEIHNKVFDLGFVKEEFGE
                     KILARENVFPTGLNLGDYGVAIPHTDAEYIKEQFISVCTFNEPVVFSSMEDQDEKVPV
                     NLAFVLGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLKTLKSL"
     gene            842..1117
                     /locus_tag="MJE33_002863"
     CDS             842..1117
                     /locus_tag="MJE33_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY9648786.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            1176..2450
                     /locus_tag="MJE33_002864"
     CDS             1176..2450
                     /locus_tag="MJE33_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012951111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBY9648787.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKFVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLFLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPE
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            2580..3632
                     /locus_tag="MJE33_002865"
     CDS             2580..3632
                     /locus_tag="MJE33_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBY9648788.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DDHGMLGAKSFGAFAEYVNIKATNVLPIGDMDFEDAAMIEPLAVAMHGVLNIGVQVGD
                     TVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDINPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            3632..4663
                     /locus_tag="MJE33_002866"
     CDS             3632..4663
                     /locus_tag="MJE33_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBY9648789.1"
                     /translation="MKAVVKTKPGYDNVEVLEVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKNIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCPTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EESLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMASGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            4755..5567
                     /locus_tag="MJE33_002867"
     CDS             4755..5567
                     /locus_tag="MJE33_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBY9648790.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVNPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            5570..6490
                     /locus_tag="MJE33_002868"
     CDS             5570..6490
                     /locus_tag="MJE33_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBY9648791.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILASIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            6665..7111
                     /gene="rpiB"
                     /locus_tag="MJE33_002869"
     CDS             6665..7111
                     /gene="rpiB"
                     /locus_tag="MJE33_002869"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBY9648792.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            7141..7806
                     /locus_tag="MJE33_002870"
     CDS             7141..7806
                     /locus_tag="MJE33_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBY9648793.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTE
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
     gene            7916..9418
                     /locus_tag="MJE33_002871"
     CDS             7916..9418
                     /locus_tag="MJE33_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBY9648794.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVKKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGAIMITASHLPYYYNGLKFFTESGGLEKTDIKEMLDIAVKNDSVDYEEVNKKGEV
                     IVKNLIEDYSNLLIDKIRKGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNKSDLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRPYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
     gene            complement(9730..11322)
                     /locus_tag="MJE33_002872"
     CDS             complement(9730..11322)
                     /locus_tag="MJE33_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBY9648795.1"
                     /translation="MVTKLKNLIINDSKAKFILLLLLFLLVSILFEAVYDLNNNFSYN
                     INCYYKEFALPYFYFFGTPVHLSKVELEFVCIGIILSSFLLTILCFTLYKKNKQTKVK
                     FLENVISLFSKVKIELTLILTLILLTLRFHAFTSNKMFYNTNLIIYNFIIISIVYIIY
                     ENIKYNNYNLKDMSITYVFFSNLLSMYNKKTINKKLAIVFLISIVVQAIIQYLVLIFF
                     MYIYYAEAIAIVILFTLVNTVFHFYIYNFLSVKFNYINNISKNIKNIENGNLKYKLKV
                     IGDDEISTLAKSINNITGGLETAVDANIKNERMKAELITNVSHDLKTPLTSILSYVDM
                     LKNNDFDRETINDYINILDKKSQRLKLLIDDIFEASKLSSGDVEFNITKTDIRELLIQ
                     SIVEFEDKIQNSSLDFIIETPEYAVFTMIDGKKTWRVFENLICNILKYSMPNTRVYID
                     MFIKEENIILTFKNISNDKLNLKPEELIERFRRGDISRKTDGSGLGLSIAQNIIELEN
                     AHMEIIIDADLFKVMLTFRRVN"
     gene            complement(11291..12007)
                     /locus_tag="MJE33_002873"
     CDS             complement(11291..12007)
                     /locus_tag="MJE33_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY9648796.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSHEIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
     gene            complement(12547..13347)
                     /locus_tag="MJE33_002874"
     CDS             complement(12547..13347)
                     /locus_tag="MJE33_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBY9648797.1"
                     /translation="MTFKNKKFTINKYKILSIILVLCLILTAFSSYVNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNHVFVVLVSTGCGISVFDKYKKYENLTNIQK
                     SEYRNREFLASLNSLGVIRENIILLPETNTNGNTDFNYMEKIALTFEEKFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNAGKIKDAKYFVKPKFADKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEYREGIGYKSDHKSFDKLLNNKDVPAILHTPNL"
     gene            complement(13661..14392)
                     /locus_tag="MJE33_002875"
     CDS             complement(13661..14392)
                     /locus_tag="MJE33_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648798.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLHDNPHDRSLFIASLN
                     AVMKYLGKTDRTIHCKNNEPEVCAKKFPEFIKMEFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLERLCFCSK"
     gene            complement(14379..15074)
                     /locus_tag="MJE33_002876"
     CDS             complement(14379..15074)
                     /locus_tag="MJE33_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBY9648799.1"
                     /translation="MLKIKNLSLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMSVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALIAEPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            complement(15076..15867)
                     /locus_tag="MJE33_002877"
     CDS             complement(15076..15867)
                     /locus_tag="MJE33_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9648800.1"
                     /translation="MKNKFFTTLLTGFFAIVFLFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAITLSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKIFKLDFIFTKQGIVLAQIFVNLPLTIKILHTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFISLTCLMLFEIFFNREVDKN"
     gene            complement(15877..16689)
                     /gene="modA"
                     /locus_tag="MJE33_002878"
     CDS             complement(15877..16689)
                     /gene="modA"
                     /locus_tag="MJE33_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBY9648801.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKENKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAKKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
BASE COUNT         6418 a         2071 c         2712 g         5652 t
ORIGIN      
        1 attcataata aataatttta taaaatagtt gttaaacata aaaaaccatt tgtaaaaaac
       61 aaaaaaatca tttctctaac aaaaaaaacc acaaaaaata taaaaaagta tggctagtta
      121 agtgattaac aagtaaaaat taaaataaaa atcaaaaaaa atttatcaat gaaagtcagc
      181 taaaatcctg gaagagagca ttatttttgg gaaaacattt tcttttgttt cacaaaaaaa
      241 gccacttttt ggcacacaac atgctttata taaagtcaag aaaaactaag gtataagaca
      301 aaggcaatta ggaggtgtaa atatggagtt tataaatttt gtaagtgata aagttataga
      361 ccttaaaatg gattgtaaaa cgaaagaaga tttctttaaa gaaatacata ataaagtttt
      421 tgatttaggc tttgtaaaag aagaatttgg agaaaaaata ttagcaagag aaaatgtatt
      481 tccaacaggt ttaaatttag gtgattatgg agtagcaata ccacatacag atgcggaata
      541 tattaaagag caatttatat cagtctgtac ttttaatgaa ccagtagtat ttagttctat
      601 ggaagaccaa gatgaaaaag tacctgtaaa tttggctttc gtattaggac ttaatcaacc
      661 acacagtcaa ttatctgtat taacagaatt aatgggaatt atgcaaaata aagagttagt
      721 tgataagttg atgagttcta ctgataaaga agaagtttta aagacactaa agtctttata
      781 aaaataaaaa agtactaaaa ataaaaaata taaaaataaa aattattagg aggaaataaa
      841 aatgaaaaaa atattagtag catgtggagc aggaatagca acatcaacag tagtttgtga
      901 caaggtagag agattagtta aggaaaataa tatacaagca gaagttatac aatgcaaaat
      961 agctgaatgt gcaacaaagc aagaaggagc agatttaata gtatcaacaa ctatactacc
     1021 aacaacttat aatataccag caataaaagc aacagcatat ataactggag taaatacagc
     1081 agcattagat aaaaaaatat tagatgcttt aaaataatcc tatcatagat acattgaaat
     1141 aatattttat aaaacagaaa aaggaggatt tataaatgga taaattatta gcagtagtac
     1201 aatacatttt aaatatggga ccgacagtaa tactaccaat aacaattgta gtactaggaa
     1261 tgatatttag aattaagttt acaaaagctt taaagtcagg tcttacaatt ggtataggat
     1321 ttgtgggtat aaacttagtt gtaaacctat taacatcaac actagggcca gcggctcaag
     1381 gaatggttga aagattcgga cttaatttaa cagtaataga tgttggttgg ccaacaacag
     1441 catcagcaac atgggcttca ccagttgcac caatattaat accagtttgt ttattagtaa
     1501 acttactatt attatacttc aataaaacaa agatattaaa cgttgatata tggaattatt
     1561 ggcactttat agtagcaggt gcagttggat atatagtaac aggaagttat gtttgggcta
     1621 tattctgtgc aatactaatg gagttattag tactgtttat tgcagaaaag acagcaaaaa
     1681 atgttcagga gttttatgat ttagaaggta tagcattacc aactggttca acagcttcat
     1741 ttgctccact tggatattta gtaggtaaat ttgttgaaaa aataccagga ataaataaaa
     1801 tacaagcaga cccagactct atacaaaaaa gatttggtat atttggagaa ccaatgatga
     1861 tggggattat actaggtata atacttggag cacttgcggg atatgatgta gcaggaatat
     1921 tcaatgtagg tatatcaatg ggtgcagtaa tgttacttat gcctagaatg gttagaatat
     1981 taatggaagg tttaattcca atttcagaat caataagaga tttcttacaa gcaaaatatg
     2041 gagacagaga actttatata ggtctggatg cagcagtagc aactggacat ccagcagtta
     2101 tatcaacagc tcttatactt gttcctataa ctttgttctt agcagttata ctaccaggaa
     2161 acaaagtttt accatttgga gacttagcga caataccatt ttatatggca tttatagtgt
     2221 gttttagaaa aggtaatata gttcaatcag ttataacagg aacaatagta atagctatat
     2281 cactttatat ggctacaaac tttgcagatg ttcatacaca attattacaa caagcaaata
     2341 tgatgccaga aggaacaaca atggttacaa gtatagatac aggtggaaac ttgctaaaat
     2401 ggggaatatt gaaattatct caattagttt cttcatttgg aatattctag tatgtagtta
     2461 atagagatta ctctttttta gtaaagtatg cgaaaattta acatttagca gctactaaaa
     2521 aagagtaata ttttttataa atttttaaaa atacaaaata caagaggagg agatttatcg
     2581 tgaaagcagc agtattacat ggcacaaatg atatgagatt tgaagatata gaaataaaac
     2641 catgtgaaag tgatgaagta aaaataaaag ttatggcagc aggaatatgt gggtcagatc
     2701 ctccaagagt attaaaacat tggaaatatc cagttccagc tataccagga catgagtttt
     2761 ctggagtaat tgctgaagtt ggtaaggatg taaaaaatgt aaaagttgga gatagagtag
     2821 ttgcaattcc ttttatacct tgtaatgaat gtgaatattg taaaagagga ttattttcat
     2881 tgtgtgatga ccatggaatg ttaggagcta aaagttttgg agcttttgca gagtatgtaa
     2941 atataaaagc tacaaatgta cttccaatag gagatatgga ctttgaagat gctgcaatga
     3001 tagagccact agcagttgct atgcatggag tacttaatat aggtgttcaa gttggagaca
     3061 ctgtagccgt aatgggtagt ggaacaatgg ggcagttggt aatacaagga cttaaaatag
     3121 caggggcagg aacaattata gcagttgata tatctgataa taaattaaga gaatcaaagg
     3181 aattaggggc tgatataata attaatgcta aagatattaa cccagtagaa aaaataaaag
     3241 aacttactgg tggaaaaggt gttgacatag ctcttgaatg tgcaggttca aaaataactc
     3301 aagaacaatg tttacttata actaaaaaga aaagtaagat aggattttta gggatagcat
     3361 attcagatat aactttatca gaagaagcat ttgaaaatat atttagaaaa gaactagaat
     3421 taaaaggttt ttggaactct tattctgcac catttccagg acaagaatgg actaagggaa
     3481 tcaatctagt taatgaagga aagataaaat taaaagaaat ggtatctcat agattctcac
     3541 ttgaagatac ttacaaagca tttgaaatga taagagatag aaaagaagaa tttaacaaaa
     3601 tattaatttt accacaaggg gttgaaaaat aatgaaagca gtagttaaaa ctaaaccagg
     3661 ctatgataat gtagaagttt tagaagtaga agaaccgaaa gctacaggag acaaggttaa
     3721 aataaaagta gaatatagtg gtatatgtgg ttcagatata cattcattta aaggtgaata
     3781 tgcaaatata aaagccccag taacattagg tcatgaattt tctggaattg ttgtagaagt
     3841 aggagaagat gttaaaaata taaaagtagg agatagagtt acatcagaga ctacttttgc
     3901 aacttgtgag aaatgtatat attgtgaaac taaagattat aacttatgcc caactagaaa
     3961 aggtattggt actcaagtaa atggaagttt tgctgagtat gtattatcaa gagaagaaag
     4021 catacatgta cttcctgaaa atgtatcttt attatcagca gcccttacag agccactagc
     4081 ttgttgtgtt catgcagctc ttgaaaagac tactatagaa agttctgata cagttttaat
     4141 aataggacca ggaccaattg gtttattatt agctcaagtt gttaaatctc aaggtgctac
     4201 agttataatg tctggtgtta caaaagataa ggaaagatta gaaatagcta aaaatcttgg
     4261 agtagataga actgtaaatg taatggaaga aagtttggca gatgtagtga atgaaatgac
     4321 tgatggaaat ggagtaaaca aaggatttga ttgttctggg tttatgccag cagttaacga
     4381 agccttaaga ctgcttagaa aaaaaggtac tttcgtacaa gttggaatct ttgctaaaaa
     4441 attaaatgaa atggaccaag aagcaataat ccaaagagag ttacaatata taggaagtag
     4501 atctcaaaag ccaagttctt ggattacagc attagattta atggcaagtg ggaaagtaaa
     4561 tacagaagct ttagttacta gaactgttgg tttagacgaa tttaaagaag gtattacagc
     4621 tttaatgaac ggggaagaaa taaaagtagc agtaaaatct taaaaaaaca tttgtattat
     4681 tttaaaaaaa attgtaaaat ctactgtagg ataaaaaaat gtaaggtctt aaaaaataac
     4741 aggaggaaga aagtatgagc actcttagag atcattctaa taatattaga aaaaacataa
     4801 taaaaatggt tgcagaagct aaaagcggac atccaggtgg ttcattatca atagctgata
     4861 tgttgacagt attatatttt gataagatga acgtatgtgc agaaaatcca aaaatggctg
     4921 atagagatag atttgttcta tcaaaaggtc atgcagcacc agctttatat gctacattag
     4981 cagaaaaagg atttttccca gaagaagaat taataacact aagaaaattt ggttctaagt
     5041 tacaaggtca cccagacatg aaaaaagtag caggaatcga tatgtctact ggttctcttg
     5101 gtcaagggct atctgcagca aatggtatgg cacttgcagg aaaattagat aacaaagact
     5161 atacagtata tactatatta ggtgatggag aaatccaaga aggtcaaata tgggaagctg
     5221 caatgacagc atcacattac aaattagata atttaattgc atttgttgac ttaaatggat
     5281 tacaaataga tggttctaat gaagaagtaa tgaatgtaaa tccagtagat gacaaattca
     5341 aatcttttgg atggaatgta atagtaataa atggacattc ttttgaagaa ataggaaatg
     5401 cagttgatga agctaaaaaa gttacaggaa aaccaactgt aataatagct aaaacagtaa
     5461 aaggtaaagg tgtttcattt atggaaaata atgctgcatg gcatggaaca gctccaaatg
     5521 cagaacaagc agagcaagca ttgaaagatt tagaaggagg tctataggaa tgggaatagc
     5581 aactagagaa gcttacggac aagtgttaaa agaacttgca gaaaataaag atatagtagt
     5641 attagatgca gatttaggaa aagctacaaa aagtatatca tttaaagaag ttgccccaga
     5701 tagatttttt gacatgggga tagcagaagg tgacatgatt ggtacagcag caggtcttgc
     5761 tacttgtgga aaaatacctt ttgcgagtac ttttgcaata tttgcagcag gaagaggcta
     5821 tgagcaaata agaaactctg tagcatatcc aaatttaaat gtaaaaatag cagctactca
     5881 tgcaggtgta acagtaggtg aagatggtgg tagccatcaa gctatagagg atatatctct
     5941 aatgagaggt ataccaaata tggttgtatt aaatccagca gatgctttag aagcaagaca
     6001 agctattctt gcatctattg attataatgg acctgtttat ataagattag gtagagctgc
     6061 aactcctgat gtaaacagtg aaaattataa atttgaaata ggaaaaggaa ctgttttaag
     6121 agaaggttct gacataacag taatagctac aggaataatg gtagcaaaag ccttagaagc
     6181 agcagaagaa cttgctaaag aaggtataaa tgtagaagtt gttaatatat ctacaataaa
     6241 accattagat gaagcactaa taaaagaaac tgctaagaaa actggaaaag tagttacagc
     6301 agaggagcac agtataatag gtggtttagg ttcagctgta tgtgaagctt tagcagaaac
     6361 taaagatgta gtagttagaa gaattggtgt taaagatgta tttggtcaat caggtacacc
     6421 agctgattta ttaaaacact atggtttaac tacagaagat atagtaaaaa atataaaaga
     6481 gttgttatag atataaatat atctaaataa caaattgatt tgacataagt caattaaagc
     6541 tgtcttaaaa atagaaagaa atttctacaa tgggacagct ttatttttac aatactttat
     6601 ttttatgata attataaaat agtctgaaag taatcaaaaa aattataaaa agaggtgcta
     6661 tttaatgaaa atatctattg gatgtgacca tggtggttat gaattaaagg aatatataaa
     6721 ggaaaattta ataaataaag gtatagaagt tgaagatgtg ggtacaaatt caaaagaaag
     6781 tgtagatttt ccaatatatg caaaaaaggt aagtcaaaat gttcaaagta aaaaatctga
     6841 tttaggcata ctttgttgtg gaacaggaat aggtatgtct attgcagcaa ataagtttaa
     6901 aggaatcaga gcagctgttg tttctgactg tttttcagct aaggcaacta gagaacacaa
     6961 taatacaaat gtactttgtt taggggaaag agttattggg aaaggattag caatgagaat
     7021 tgttgaagaa tggttaaatt caagttataa tggagaaaga catgcaagaa gattacaaat
     7081 gattgatgat atagaaaaag aaaatttata aatatataat ttataacaag ggggagcgtt
     7141 atgtctatta tttgtgcttc aataatgtgt gcagaccaaa tgaagttaaa agaggaatta
     7201 gaagctcttg aagaagcagg tgtaaaatta cttcattgtg atgttatgga tggaatattt
     7261 gttaaaaact tggctatggg accagagtta ttaaaatcaa taagtgaaaa tacaacaata
     7321 cctttagaca tacacttagc cacagaaact ccagataaat atattgatat gatgtcttat
     7381 ataaagccaa aatatatttc ttttcatgta gaatcaagta caaatgtaaa agctgatata
     7441 caaaagttaa gaaattatgg tatagggcca gtgttagcaa taagccctca aacaagtgtg
     7501 gataaaatag aagaatatat aagtttagtt gagggtatct tagtaatgac agtaaatcca
     7561 gggtttgcag gtcaaaagtt taatctttct gtattggata agttggataa acttacagag
     7621 atactaaaag attatgataa tcctccattt atagaggttg atgggaatat aaataaggat
     7681 actattaaac ttatgaatgg taaaaaagta gatatatatg ttgttggtac ttcagcttta
     7741 tttaatgata agcctccaat ttcatataaa gataaaatag aagaactaaa agaaagtata
     7801 aagtaaccat gtaataaaat tataatatgt ttctgtttaa tagtaaaaat ttaggtggaa
     7861 acaagaaatt ttataatatt taataaaaat tttggttgag taaggagaat tttttatggg
     7921 agacctatat aaactacaaa atggtactga tattagagga atagcgtatg aaaatgattc
     7981 taaagaagtt aacctaactg tagaagaagt aaaaaaaata gctaaagcgt ttcatatttg
     8041 gttaaaagaa aagactaaga aagataaagt aactgttgcc ataggaactg attctaggat
     8101 aacaggaagc caatttagaa gtactgttat tgaaacttta actaatgacg attgtaatgt
     8161 aattgattgt gagattgcca caactccagc tatgtttatg acaactatta tggatggata
     8221 caattctgat ggggcgatta tgattacagc aagtcactta ccttattact ataatggttt
     8281 aaagtttttc acagaaagtg gaggtttaga aaaaactgat ataaaagaga tgttggatat
     8341 agctgttaaa aatgatagtg tagactatga ggaagtaaat aaaaaaggtg aagttatagt
     8401 aaaaaattta atagaagatt attctaattt attgatagat aaaattagaa aaggtgtaaa
     8461 ttcaagtaaa aattatgaaa agcctttttc tgggcttaag atattggttg atgcaggaaa
     8521 tggggctggt ggattctttg ctgaaaaagt tttacacata ctgggagcag atacgacagg
     8581 tagtcagttt ttgaacccag atggtatgtt cccaaatcat atacctaatc ccgaaaacaa
     8641 agaagcaatg gaaagcatat gtaaagctgt gttggataat aagagcgatc taggaataat
     8701 ctttgataca gatgtagaca gagctgctat tgtaggtaaa aatggcaaac caataaataa
     8761 aaatgctctt atagctgtta tatcatcaat agtattagaa gaacatccaa aaacagctat
     8821 agtaacagat tcaattactt ctgaaggact tgctaaattt ataaatgaat tacaaggaag
     8881 acatcataga tttaaaagag gatataaaaa tgtaataaat gaagctataa gattgaatag
     8941 tgaaggtgaa gagtgccatt tagctataga aacgtctgga catgctgctt taaaagaaaa
     9001 ttatttctta gatgatgggg catatttaat agctaaaatt ttgataaaag tagcaaagct
     9061 atccttggaa ggaaagacta tagaagaatt gatagaaaac cttgaagaag ctcaagaaga
     9121 gaaagaagtt agaataggca ttaataaaga agattttaga ccatatgcag aggatatatt
     9181 aaaagattta gattctcatg ttaagagtat tgatggatgg tctgtagcac caaataattt
     9241 tgaaggcata agagttaatt gtgatgaatc aaatggagat ggatggtttt tacttagaat
     9301 atcactacat gaaccattat tagctttaaa tattgaatca aataaaattg gtggagcaga
     9361 acaaatttat tctaagttaa aacttttctt agaaaaatat gatttaaaag gtctttaggg
     9421 taaatagaaa gaatatattt atattgaaga ataaatgtaa atttgtacct aagattacaa
     9481 atattacaaa attgtaatct taggtaacag agatgtaact gattatggaa acactatgtg
     9541 atattataga tacagagaga tgaaatatat gatgaattat agagaaatat aatttaaaat
     9601 gtattaatat cacaccaaaa aggtataatc aaaaaatatg taaatataaa ccccaacatt
     9661 tagaaaaaga cctctaagga ggtttttttc ttgtttaaat aacttaaaat atatttttat
     9721 attaatatat caattaactc ttctaaaagt aagcattacc ttaaataaat cagcatctat
     9781 aattatttcc atgtgtgcat tttctaattc aattatattt tgagcaattg ataaaccaag
     9841 accagagcca tctgtttttc tagatatatc accccttcta aatctttcta ttaactcttc
     9901 tggctttaga tttagtttat catttgaaat atttttaaac gttaatatta tattttcttc
     9961 ctttataaac atatctatgt agaccctagt gttaggcata gagtatttta gtatattgca
    10021 aattagattc tcaaaaactc tccaagtttt tttaccatct atcatggtaa aaacagcata
    10081 ttcaggtgtt tctattataa aatctaaact gctattttga attttgtctt caaactctac
    10141 aatactttga attaaaagtt ctcttatatc ggtttttgtt atattaaatt ctacatctcc
    10201 actacttagc ttagatgctt caaatatgtc atctattaat aattttaatc tttgtgattt
    10261 tttatctaaa atatttatat agtcatttat agtctctctg tcaaagtcat tattttttaa
    10321 catatcaaca tagcttaata tagatgtaag tggagttttt aagtcatgag atacgtttgt
    10381 gataagctct gccttcatcc tttcattttt tatattagca tctacagcag tttcaagacc
    10441 gcctgttata ttatttatac ttttggctaa agtagatatt tcatcatctc ctataacttt
    10501 taatttatac tttaaattgc cattttctat attttttata ttttttgata tgttatttat
    10561 gtaattaaac ttaacagata aaaagttgta tatataaaaa tgaaaaactg tgtttacaag
    10621 tgtaaataaa attactatag ctattgcctc agcataatat atatacataa agaatatcaa
    10681 aacaagatat tggataatag cttgtacaac tatagatatt aaaaatacta tagctaattt
    10741 tttatttata gtttttttat tatacatact aagtaagttt gaaaagaata catatgttat
    10801 ggacatatct tttagattat agttattata tttaatattt tcatatatta tatagactat
    10861 agatataatt ataaaattgt agattattaa attagtgttg taaaacattt tattacttgt
    10921 aaatgcatga aaccttaagg ttaatagtat caaagttaat ataagtgtaa gttctatttt
    10981 taccttagaa aaaaggctta taacattctc taagaatttg actttagtct gtttattttt
    11041 tttatacaat gtaaaacata gtatagtcaa taaaaaactt gataatataa tacctatgca
    11101 gacaaactct aattctactt ttgacaaatg tacaggagta ccgaaaaaat aaaaataagg
    11161 gagagcaaac tctttataat aacaattaat attataagaa aagttattat tcaaatcata
    11221 aactgcttca aataatatag aaactaatag aaacagtaat aataataaaa taaattttgc
    11281 tttagaatca ttaataatta agtttttcaa ttttgtaacc aatgccccac accaccttta
    11341 aatattttgg ttcttttgga ttaatttcaa tcttttctct aattcttcta atatgaacag
    11401 caatagttct ctcacaacta taagcatcct cattccagac attttcataa atctcatgag
    11461 aagagaaaat tttacctttt cgtgacataa gaagtgtaag tattttatat tcaagtggtg
    11521 tcattttaat ttcctcacca tctaaagaaa ctatttttgt atctgtattt agctctaggc
    11581 caccactttt tagaatataa ttagtatcat ttgagttatt tgtattacta aaatcaccta
    11641 aatttatata ccttctaagt tgagatttta ctcgagcagt aagttctagt ggattaaatg
    11701 gtttagttat ataatcatca gcacccatat taagacctat aattttgtcc atatcttctg
    11761 attttgcaga taagagaatt attggtatat tccttttttc tcttatttta agtgtagttt
    11821 taagcccatc taaattgggc atcattacat ccattattat taacttaata tcattttcga
    11881 ttagtatgtc caatgcttca agtccatcat atgcctttaa aacattaaag ttatcaggtc
    11941 tcaaatagaa ctctatagca tcaactatct ctctatcatc atctacaacc aatatatttt
    12001 gattcaactt attaaccccc ttgaaatttt atttttaacc aatttttatt ttataccaaa
    12061 aagaggtatt agaaaacagt aagcccgttc attatgaaat aaaaactagt acttttaata
    12121 tattgtcaat gcatatagta aatttaaaag tatagacgcc catattaatc tgaaggtaaa
    12181 agtttatgaa aatatctagg gtaatctatc acacttctta tgttattttg gtttattaat
    12241 tttttaggtt taaacatata ttaacaaata taaataacaa atgagtttca taattattaa
    12301 gaatttataa caaatattta ttttaatgta gtttaatata aacaggtagt ttaatgtagg
    12361 tatgtataaa gcatttaata aggcttatag gttttaatga tgatatcttt atatatttta
    12421 agagatgatg aaattagata tgggtaatag ttgtatgaga aaaagtagta tagcacaaaa
    12481 agtgctatat cacaaaatct caaataattt cctttttata acttaaatta aaagaataaa
    12541 tatttcttat aaattaggtg tatgaagtat agcaggaaca tctttattat ttaataactt
    12601 atcaaatgat ttatggtcag acttatagcc aataccttct ctatattcta attcatcaac
    12661 aattttgtat tgttgacagg catttttaac cttttcatag tcttcagtag aatgagcttt
    12721 ataaactatc ttattattaa aagttatttt atcagcaaat ttaggtttta caaaatattt
    12781 agcatctttt attttaccag cattatataa attttggata acagaaccat tttttaaatg
    12841 ttgtaaatgg tcatctaact tataagtatg tgctatatga gttacatttt taaatttttc
    12901 ttcaaatgta agagcaattt tttccatgta attgaagtca gtattaccat ttgtatttgt
    12961 ttctggtaat aatattatat tttcccttat tacaccaagg ctattaagag aagctaaaaa
    13021 ttctctattt ctatattctg atttttgtat atttgttagg ttttcatatt ttttatattt
    13081 atcaaagacg gatataccac atccagtaga tactaaaacc acaaatacat gattttttcc
    13141 tctttctttt atagcattaa caatagcact cccagcccac aacacttcat catcttgatg
    13201 ctgaacatag aagacaacat aatctttaaa ttttttatga ctataattca catagctact
    13261 aaaagcggtt aaaatcaaac ataaaactag aataatagat agaattttat atttatttat
    13321 agtaaatttt ttattcttaa atgtcattta atcatctcct gaaaagttgt cttagttata
    13381 ttatcaaaca aagtactaaa aattatttct taaatgctga aaaatataga gtagattaat
    13441 ggaataaaat ttttttatta gtttaaaata aagaaaacat ttatgtataa gactaatcaa
    13501 aatattaatg attgatagga attgagattt aaagggattg tacatttatt cgggattcat
    13561 tttaatagaa ttttacttta gtacttagaa taaaagtaat aactttttaa atttaaagag
    13621 tacacatata tatttgtgta ctccttgatg atatgattgc ttatttacta caaaaacaga
    13681 gtctttctaa acctaaaata tttgatgcac cagctatcgt agttccataa tagtaaactg
    13741 gtttgttaag tgatagaaaa tttataattg agttattaca taatgtactt cctgtgcaaa
    13801 tcactaaatc agcccaagat attacatctt cataatctcg tataccatct tcaatcttaa
    13861 cattatattg aatagtatcc acaaattcag gatttaaatc taatactctt gtttcgaaaa
    13921 agtccttaat attatcaatt atggctggtt gatatcctat aattgctact tttggattac
    13981 caaattccat ttttataaac tcagggaatt tttttgcaca tacctctggc tcgttatttt
    14041 tacaatgaat ggttctatcc gtcttgccta agtatttcat aactgcattt aatgatgcta
    14101 taaaaagact tctatcatga ggattgtcat gtaaatctaa tgataatatg tcttttaaag
    14161 aaccttcaaa tgtagatgga gtagaagtaa atgcttgtcc tttagctcct ttaaagtcag
    14221 cttcaagcat tatttcttta ccaactataa ttggaaaatc tttacgctta gtatttccaa
    14281 ttgcttcttt tgaagataga gatttggaca caatacttat catatccata tttaaattat
    14341 atttatcgac ttgtactaat agataatctc tcaaaatatc aaatatattc atagaaaaaa
    14401 gattctcctt tattttttat ttcttcattt aaatcaaaaa tatgatttat ttttccattg
    14461 ttcattattc ctatcttatg agcaaaaaac attgcttcag aaatatcatg agtaacatga
    14521 atcactgttg gtttgtattt tatgtagaca tccttcaaaa ttttgcttat atgttttttt
    14581 gtaatataat ccaatgaact cataggttca tctaaaagta atatatcagg ttcagctatt
    14641 aaggcacgtg caaaagcaac tcgttgcttt tcgcctccac ttaaattttt aggataccta
    14701 ttttttatat cttctatctg cattaattta gacaagtggt tagccttaag aatctgttca
    14761 tctataggta tttttcttaa tttaagtgga aaccttatat tatcttcaac actcatattt
    14821 ggaaataatt caaaattttg atatacaaat cctatatttc tattttctgg aggtaagttt
    14881 gatattaaat tttctttgta tgatacattt cctttagcag atggatatct tcctgctaat
    14941 gtttctagga atagagtttt acctgaccca ctagctccta ataaaacaaa gtattcatga
    15001 ttattaatat ttaaactatt ttcttgtaat ttaaaattag gtaaatcaag agataaattt
    15061 tttattttta acatattaat tcttatcaac ctctctatta aaaaatatct caaaaagcat
    15121 caagcatgta agtgagataa atatcaaaat tacagctatt ccgattgcaa tgtctatatc
    15181 accagtagac atatttaaat atatagatgt aggaataatt tcagttttca tacgtgtaga
    15241 acctgctatc atagctactg caccaaattc accaagtgct cttgaccaag tcattacagt
    15301 tgcagatatg attccagttt ttagatttgg aagaattata aatctaaatg cttcccaact
    15361 attacatcca agggtacgtg caacaaatat catcttttca ttactttcat ttaatgaagt
    15421 gtgaagtatt ttaatagtaa gtggaagatt tacaaatatt tgtgctaaaa ctataccttg
    15481 ctttgtaaat ataaaatcaa gcttaaaaat cttataaatg gaatctccaa tccccattct
    15541 tccaaacaaa agtaaaagag caattcctga tactaagtgt ggaagagaca tagggagatt
    15601 tattatctgt ataatcagtt ttttaaatgg taatttagta atatgtaaaa aatatgctac
    15661 tggaatagct aataaaaggc atactatagt agatattaaa gatgttttaa tactaagtgt
    15721 tattgcatac tgtacttcca ctgattttaa gcatatagga acataactta tccctttaat
    15781 gattaacata agaagtggtg atacaataaa caaaaagact atagcaaaaa atccagtaag
    15841 tagagtagta aaaaatttat ttttcaagtt taatcactac ttaacaggtt caaaaccata
    15901 ttttttaaat atctgtttag cttcgtctga ctctatataa tttataaatt tattagctaa
    15961 atctttttta tcactatagt ttacgattcc tgctgatata agttggtcta tatttttatc
    16021 ttgggcaatt tctataactt ctacatcttc attaccaaag accgaatctt ttgtggctat
    16081 tgtagcatca gctttacttg atgttaactg tacaatcatt tcatttacag ttgcagctgt
    16141 agagacaaca ttatcattta tagattttaa attatttttt tcaagtattt tttgtgtagt
    16201 cttacctata gcattagact ctttgtctcc caaaataagt ttaactccat ctttatttaa
    16261 gtcttccaat ttttgaatat tagctggatt tcctttaggt acaactattg caggtgtatg
    16321 atgtgcaacc gttttatgtt cacctacaag ttctttttca aaggcctttg aataaattgg
    16381 ttcagaacca acaataaata cgtctccttt gtgacttgtc tcaatttgag ctattagctg
    16441 tgcagaacct gcatagctat attcaacttt gacattattt tctttctcaa atttttttgc
    16501 tatttcatcc attggtttct ttaaacctgc ccctgaatag acaaaaagag tctctttttc
    16561 ttttgaatca tcagctgttg tcactttagt attagattca tcattagctt tgttttcttt
    16621 agaattacat gcagtaatac ttaatgaaat aatcatagct aaagctagta ctgaaagttt
    16681 tttaaacata agatattccc ccttagtaaa ttagtgtaaa ttctttttta gtatatcata
    16741 ctaagaaaac gatgtctaag tttttgttat gttttataat gttttgttat gttttgttat
    16801 gtatagttaa gaaaaaatct atttataatg tcatttccat aggtagttat ttt
//
LOCUS       DAGSRM010000086        16376 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.086, whole genome shotgun sequence.
ACCESSION   DAGSRM010000086 DAGSRM010000000
VERSION     DAGSRM010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16376
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.086"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(21..1412)
                     /locus_tag="MJE33_002879"
     CDS             complement(21..1412)
                     /locus_tag="MJE33_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBY9648802.1"
                     /translation="MYKLIDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKYP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEIINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLLPL
                     PKKSISLLIPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYMMLIKNIKLA
                     FDPKNILNPGKIY"
     gene            complement(1423..2643)
                     /locus_tag="MJE33_002880"
     CDS             complement(1423..2643)
                     /locus_tag="MJE33_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HBY9648803.1"
                     /translation="MSKIVVNQDKITDLKRILEICPFGAIEEKSGIVEISAGCKMCKL
                     CVKSGPKGAFEFIESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCSNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFIDKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSAPEKIENTTGKITLCKVEKKELKSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMIYKLADKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDNNQKYMASAAK"
     gene            complement(2657..3445)
                     /locus_tag="MJE33_002881"
     CDS             complement(2657..3445)
                     /locus_tag="MJE33_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBY9648804.1"
                     /translation="MEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYALET
                     ALRIKEDKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSYTIS
                     QGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEVKDESIVVEID
                     MPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLNTKDREIPVYSLDDFEDKDENNYG
                     LNGSPTQVVRIFPPKPNTDKNIVRGNADELSFALVNKLEELKLV"
     gene            complement(3462..4736)
                     /gene="larA"
                     /locus_tag="MJE33_002882"
     CDS             complement(3462..4736)
                     /gene="larA"
                     /locus_tag="MJE33_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HBY9648805.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRNVNPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHISTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSVLPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTELSKVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMISACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTIPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEMANSIVGKESKVVVIPDGVSVIVK"
     gene            complement(4854..5567)
                     /locus_tag="MJE33_002883"
     CDS             complement(4854..5567)
                     /locus_tag="MJE33_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBY9648806.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLEDILEL
                     RIILEIEIAKLASKRITSSEVIELKHIIDEMRVETNKKDNNRVLVLLDQKFHSKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEN"
     gene            5692..6951
                     /locus_tag="MJE33_002884"
     CDS             5692..6951
                     /locus_tag="MJE33_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HBY9648807.1"
                     /translation="MKKNKIIAFFAPSIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRFVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNGQISKYEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNENLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSSPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGVLAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTILQIVVIIYLISAVFIAKK
                     HMKTLSTNGKLMRYKNTFFTKKFSRKK"
     gene            7231..7797
                     /locus_tag="MJE33_002885"
     CDS             7231..7797
                     /locus_tag="MJE33_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBY9648808.1"
                     /translation="MATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALALM
                     LEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYNLK
                     SNDYLVNIKTKAIQKQLRIICKYLNLYNISTHSFRKLYATTQFEKSNNNLELVKELLN
                     HSSVATTQRYIRVTQQAINEASENFFIG"
     gene            complement(7998..9203)
                     /locus_tag="MJE33_002886"
     CDS             complement(7998..9203)
                     /locus_tag="MJE33_002886"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBY9648809.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNTSKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNNVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDNNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRSSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNKDGILECLDKYVDSYDIIDDEELAIFVSAKSEHEIDRVLEIVKRNNYSE
                     SAKKLLKID"
     regulatory      complement(9306..9412)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(9504..9872)
                     /locus_tag="MJE33_002887"
     CDS             complement(9504..9872)
                     /locus_tag="MJE33_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HBY9648810.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDTKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     gene            complement(9924..11201)
                     /gene="obgE"
                     /locus_tag="MJE33_002888"
     CDS             complement(9924..11201)
                     /gene="obgE"
                     /locus_tag="MJE33_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBY9648811.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGIGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELEGRGYTVFKMSAATRQGIDEVIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            complement(11312..11602)
                     /gene="rpmA"
                     /locus_tag="MJE33_002889"
     CDS             complement(11312..11602)
                     /gene="rpmA"
                     /locus_tag="MJE33_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBY9648812.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            complement(11616..11933)
                     /locus_tag="MJE33_002890"
     CDS             complement(11616..11933)
                     /locus_tag="MJE33_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBY9648813.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECIVKDTHVKDAQLLLNHFQLAVKEIKREYPKNIKI
                     LKK"
     gene            complement(11948..12259)
                     /gene="rplU"
                     /locus_tag="MJE33_002891"
     CDS             complement(11948..12259)
                     /gene="rplU"
                     /locus_tag="MJE33_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBY9648814.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            complement(12360..13781)
                     /locus_tag="MJE33_002892"
     CDS             complement(12360..13781)
                     /locus_tag="MJE33_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HBY9648815.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNIYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSTIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCTHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRGIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYKTNLEAAKEIVRQLRIRDIAGIIIIDFIDMHKKKHQ
                     KEILHILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDSIEKEIMRISEHTVYKNIMVEFNDFIFEQIKEYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            complement(13799..14500)
                     /locus_tag="MJE33_002893"
     CDS             complement(13799..14500)
                     /locus_tag="MJE33_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HBY9648816.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGTESQGEYVDIEIEEDDLSVEEFLNKVSIQLPDGIDFIKAKEID
                     RQTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKTNLNTNILIPKILEMFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            complement(14502..16346)
                     /locus_tag="MJE33_002894"
     CDS             complement(14502..16346)
                     /locus_tag="MJE33_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HBY9648817.1"
                     /translation="MNKVDLKKILKKVEKPARYLGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENIMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLADFV
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNREGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYAHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
BASE COUNT         5041 a         3087 c         1747 g         6501 t
ORIGIN      
        1 atattattaa attaatattt ctaatatatt ttccctggat ttaaaatatt tttagggtca
       61 aaagctaact ttatattttt aattaacatc atataagcat ctgaatttga ttcatgtaag
      121 tactcttttt tagcatatcc tatgccatgt tcaccagaaa cttgaccact aagttctcta
      181 gattttttat acatatagtc aaaagtttct tttaatctta ttttccatgt attatcatcc
      241 ataccatctt ttaaaatata tatgtgtaga tttccatctc ctgcatgacc aaaacttttt
      301 attcttattt ttaatttgtc ttgaagctcg tgagtgtatc ttataaactc tgctatctta
      361 tctcttggaa caacgacatc acattcatcc atttgtgtcg tagatgcctt tatagcttct
      421 aaaaatgcac ctctggctga ccatatagaa tcgtttcttt cttgtgtatc tgatataaat
      481 acatctaatg caccattttc taaacataaa ttagctactt tttcatattc tttttctata
      541 tcttcagtgc tatttccatc aaatgtaagc aaaagataag catctgatgt attatctgga
      601 aactttttac ctaaaaattc ttctgctgcc aatatcacat ctctttccat aaattctata
      661 gccgttggta ttgattttga ttttattatt ttaggaacag tttctattgc cattgataaa
      721 tctggaaatg gtataagcaa acttatactt ttctttggta aaggaagcaa ttttaatata
      781 gctttagtaa ctattcctaa agtaccttct gagcccacta ataaatcctt tatactatat
      841 ccagaactat ttttaaccac ctttccacct acatttataa tttctccatt tggtagtaca
      901 acttcaagtc ctcttacata atcccttgta acgccatatt ttactgctct cataccacct
      961 gcattagtgt ttatattacc tgcaattgta gcactttttt cacctgggtc aggtgggtaa
     1021 aataaatctc tatcttgaac atattgacct attgtcatca ataaaacacc tggttctact
     1081 gtcaatgtta aattttcata atcaacttca agtattttat tcattttaca tagattaatc
     1141 attattccac catttatggc aacagcagca cctacaagac cagtaccttg acctcttggt
     1201 gtgactggta tattattttt atatgcgtat ttcattattt tagatacttg ttcagtttca
     1261 agtacattta ctaaaacttc agggtacttc tcaatccctc caagttcatc atgtgaaaaa
     1321 tcttcattta tatcacttcc aactaataca ttttcttctc cacaagtatc tattaaaaaa
     1381 tctatatctt ttttatcgat taatttatac atttctatcc ccctatttag ctgctgatgc
     1441 catatatttt tgattattat ctttgttatt ttctattttt tcaatcaatt taggaataat
     1501 atcataaata tcacctatta aagctaaatg tgctacatca agtataggcg ctttatcatc
     1561 tttatttatt gctactatat agtcagcacc ctgcatccct gcaacaaatt gaactgctcc
     1621 agatacacca caagttatta tcaatttagg ttttacagtt ctaccactaa gacctatttg
     1681 ttttttagca tctatccaac ctgcttctat tacaggtctt gttcctgcca cttgagcgtt
     1741 taacttatca gctaatttat atatcatctc catatcttct tgttttttta ttgctctact
     1801 tgccacaact atcacttcag cttcttccag accaacttct ttattttttt cttttacact
     1861 cagtacctta attttagatt ttagttcttt tttttctact ttacataaag ttatctttcc
     1921 tgtagtattc tctatttttt ctggtgctga aaatatttta tatcttacag tggcaaattg
     1981 tggtctatta ttaggtgtgt ttatatgagc cattatattt ccaccaaatg ctggtcttat
     2041 ttggtctaaa tcagtatttg attgaatatc aagtattgta caatctgctg taagacctgt
     2101 tctaaatcta gcggcaagtc ttggagctaa tgacctacct aatgtagttc caccaactaa
     2161 tactatcgtt ggttttattt tatctataaa gttttctatt gcttttgaat atggttctat
     2221 cctaaaatct ttaaattcat cttcatcata tacaaatact tcatccaccc cataagaaat
     2281 caaattactg cacttatctt ttatgtcttt tcctacaaat acacaataca caggttgttt
     2341 tatttttgat gccatctcac gagctttacc tataagctcg tacgttacag gatgtatatt
     2401 tccattatga tgttcaacat atactgcaat tcccctccat tcatctttat taatttgtac
     2461 tttactactc tctataaatt caaatgcgcc ttttggtcca cttttgacac acaacttgca
     2521 catcttacag ccagcactta tttctactat acctgacttt tcttctatag ccccaaaagg
     2581 acatatttct aaaattctct ttaagtcagt tattttatct tgatttacaa ctatctttga
     2641 catttattac tcccccttac accaacttta attcctctaa tttatttaca agtgcaaaac
     2701 ttagttcatc tgcatttcct cttacaatat ttttatctgt atttggcttt ggaggaaata
     2761 ttcttacaac ttgagtagga gaaccattaa gtccatagtt attttcatct ttatcttcaa
     2821 agtcatctaa gctatataca ggtatttctc tatctttagt attaagtttt aatttaaatg
     2881 aaggtagtct tggttgaaaa atgtcttttt ctacagttat taaacatgga tattcaatct
     2941 cacatacttg taaatcattt ggcatatcta tttcaacaac tatactttca tctttaactt
     3001 caattaattt tgtaacgtta gtaacatgag gtatatctaa aaattcagct acttcaggtc
     3061 ccacctgtgc tgtatcacca tcagtagttt gttttccaca aattattagg tctacctttc
     3121 ccatcttctt aataccttgt gatatggtat aggaagtagc caatacatct gctcctccaa
     3181 atctcctatc acttattaat gctccttcat cagctcccat cataaaactt tcttcaatta
     3241 ccttctttgc ctgaggagga cccatgctaa gtacttttag attcgctttc ttatcttcct
     3301 ttattctaag agctgtttct aaagcatata ggtcataggg attcattttt gaatctactc
     3361 catctctttt tagtactcct gtagtttcat caacttctac tgaagtcgtt ccaggaactt
     3421 gtttaataca tgtcaaaatt tccataaagt acactccttt tttatttaac tataactgat
     3481 actccatcag gtataactac tactttgctc tccttaccaa ctatactatt cgccatttct
     3541 aaagcttctt taaagttaaa tgcatgtttc atatgcattt tttttatcat ttcaggatta
     3601 cacttatctg ttaccattat cacagtatat ttagataata ttctcgctag tatctgaaat
     3661 tcccattggt ctggaatcgt atctactcta tcgacacttc taattttatc taaaagttgt
     3721 tttggagaat ctgcatttgc tacattttca taaaaagatt gacctccatg accatcatta
     3781 catgctgaaa tcattattat tactccaccg tctttacaag tagcttcagc tgctgtcatt
     3841 cctttaacag attgataaat attttggtct agtggatatc caccatttgt agttacaaca
     3901 atatcagatt ttatagaatt tacttttgat aactctgtta caaatctaca tcctttagta
     3961 tgtgcttttt cgacatcacc agcaaaggaa gcaattattt ttttatcttt atctattaca
     4021 acattcaata tgaatgctaa ttttgcaact ttagctgcat ataccatatc ctcatgaata
     4081 ggattattat tcaattttcc agttcttgaa ttatcactat ctataaagtc actacaatga
     4141 ttgtacatta tagtctttgc agatgcgatt cctggaagta cactttttct gccacctgaa
     4201 aaacctgcaa agaaatgagg ttctataaaa ccttctgcta tcaaaagttc tgcctcatat
     4261 gctagtttgt ttatatataa atctccacct gaaggcaata tccctacttt acacatagat
     4321 ttatcatcag tagatatatg cataactata tcttcattat ctactatttc ttctcccatt
     4381 ttataaatta gctcttccct tgtacttggc ctatgaaatc cagttgccac tataatcttc
     4441 acatcaattt ctggattaac attcctaatc cttcttaata taattggcat agttattcta
     4501 cttggtacag gtcttgtatg gtcacttgta attatgacta cattcttttt ccccttaacc
     4561 agttcttcca gagaattaga gcctatagga ttattcatag attcttcaac tatctcttct
     4621 tgagtcttat gttctatata attatccgat tttgactcca aaacaccgag aaaacgttca
     4681 tcaggtattt ctaaaatcat tccttgtttt gaatacggta cttttaactt taccattttt
     4741 aatctcccat tataataaac tataaaattg gtatgtggtc ataccagttt tacatttata
     4801 tgatagtacc attatctacc atttgcaaga ataatttatt ttataacaaa cttttaattt
     4861 tcattttttc tataattttt acttataaaa tccatatgtt cctttgcttt ctcacatgca
     4921 agttctacat cattttctac aactgcattg tatatagctt catgctgttt aagcaatata
     4981 ttttcattaa aaggttctgc gattattttt tctcttgcat cttctataaa tccatcaaat
     5041 aattttgatg ctgtcataaa aagactttga attaaataat ttttacttaa tgttgctagt
     5101 ttactgtgaa atttttggtc taataacacc agtactcgat tattatcttt tttattagtt
     5161 tctaccctca tttcatctat tatatgtttc aattctataa cttcagaaga agttattctc
     5221 tttgaagcaa gttttgcaat ctcaatttct aggattattc taagctctaa tatatcttca
     5281 agtgtaccat tattaagttt aaaaatcata gataaaggtt ctattaaagt tttttctata
     5341 tttgtacata taaaatttcc ttcaccctgt ctgctttcta tcactcccat agtctctaat
     5401 accctaatag cttcccttat ggaagttcta ctcacattca tttgttcaga cagttctctt
     5461 tctgatagta atttatcccc cttttttaac tctccattta gtattttata ttgtatttgc
     5521 tcaattactt gttcataaac ttttttacta gctatactat taaacatttt ttcctccaat
     5581 atgataatcg ttgttgtaat atatatctat tatatatctt aagccattac attcatatat
     5641 ttttattact tttaatataa tatcttataa ctcaaattta caggtgatat tatgaaaaaa
     5701 aataaaatta ttgctttttt tgctccatct atcatagttg gagttctagt tttagggatt
     5761 atcctatacc caaatgaagc tataaaatct gcaagagaag gcttttctat atggtctaat
     5821 gttttagttc catcactact accatttata attggagcaa acttaattgt tgatttaaaa
     5881 atagtggata ttatagggtt tatcatcaat cccataacta gatttgtatt taatgtttca
     5941 ggaaagagcg ctttagcttt tgctatatct acagtatctg gttatccagt aggagtaagt
     6001 ttagcttctg aatttaggag taatggacaa atttctaagt atgaagcaca aagacttgtg
     6061 tctttttgtt ctacatcagg acctttattc ataataggtt cggttggtac aggtatgttc
     6121 caaaattcaa acttaggtta tttaatgatt ctctgccatt atctaggaac tatattggtt
     6181 ggtcttttct ttagaaatta tggtaatgaa aatcttccaa agacaaaatt ggatttaaaa
     6241 acaaatataa aaaatgtaat caacattaga aattcaagtg ggaatggttt ttttgtttta
     6301 tttggaaatg ctgtatttaa tggagtaaat acattactta cagttggtgg atttgtaatt
     6361 gttttttcag tagtatttaa aatactatct ctttttaatg taatctcatt aatagcatca
     6421 gttatatatt tgcctctttc atttttaggt gttagtaaag aactctgtca agcatttgta
     6481 agtggtctat ttgaaattac tataggatgc aataaagtat ctagtataac aagttctcca
     6541 gaaattttaa gagcatcttt agctagtttt ttgataggat ttagtggatt atccatccta
     6601 gctcaatgtt gtacatttct atcaaaaacg gacatagatt taaaattata catactaagt
     6661 aaatttgcgc atggagtttt agctgctatc tttatattta tactttatcc aattgcaaac
     6721 tctgctgtgt tggtttctag ctttgctgat acatacaacg ttatatataa taatttaatc
     6781 tggttttatt atcttagcta ttatgataca attcttcaaa ttgtagtaat aatttatcta
     6841 atctcagccg tatttattgc aaaaaaacat atgaaaactt tatctacaaa tgggaaacta
     6901 atgcgatata aaaatacttt tttcactaag aaattttcac gaaaaaaata aaactaaaaa
     6961 taaaactaat aaaacttaac taaatacttc cattttttta ttatatttgt cataatttta
     7021 ataacctttt taattagttt aacagcatag ttatttcatc acaatttaat aattttatta
     7081 gaaatatttt tcaaaaaatt ctccttaatc acatatatca cacttgtata agtttactgg
     7141 gaaatttttc cattatataa tatttcattt atattatata ataaataccg acctaaactt
     7201 accaaatcaa actaagagga gtggttgaaa atggcaacaa gacctataga aatagacgaa
     7261 tataaaaaga taatggaact attacatact ggctttactt atagtgaaaa tggcgttgaa
     7321 aaaagattta gaaaaaatcc taaagttgca ttggcactaa tgttagaagc taatttaggt
     7381 ctaagaatat cagatatttt aagattaaag ataggtaatt ttaaaggtaa tatgttggaa
     7441 ataaatgaaa aaaagacggg caaattacag tatagaccag ttaataaaaa tataattgag
     7501 tccataaatg aacatgcaag aaagtataat cttaaatcaa atgactactt agtaaatata
     7561 aagacaaagg ctattcaaaa acagttgagg ataatttgta aatacttaaa tctgtataat
     7621 ataagtacac atagctttag aaagctctat gctactactc aatttgaaaa gagtaacaat
     7681 aatttagagt tggttaaaga acttttaaat cacagctctg tagccactac acagcgatat
     7741 ataagggtaa ctcagcaagc tattaatgaa gctagtgaaa atttctttat tggttaatta
     7801 taacgtatac atttttctat tatcttatta attcaccaga tattaaaaaa gagtgtctca
     7861 aaatgaactt tttagttcat gagacactct ttttttataa aatcaaatat atttttatta
     7921 tttcaacact ggaaaaaagg cttatctctc tattttgaga caccctatta aattttcata
     7981 attcttgcat aaacatatta atctatcttc aataattttt tagctgattc actgtaatta
     8041 tttcttttta caatttccaa aactctatca atctcatgtt ctgactttgc acttacaaaa
     8101 atcgccaatt cttcatcatc tataatatca taactatcta catatttatc taaacattct
     8161 aaaattccat ctttattatt atacccacca aatctaacta ctgcactaca ctcatcttct
     8221 ataagtccac ttaaatccaa tttccctttt gaaaaagact ttatatttga acttctatta
     8281 tctattatat ctataatatc tgcataaaca gcacttcctg ttgcacctct tccagctccc
     8341 ttacctacaa aagaaagttc acctactgaa tcacctacta aaataactac gttgaactca
     8401 ttatctatct ttgaaagcat attattattg tccactaatg ctggtctaac aaatccactt
     8461 acttctcctg attcatactt acttctagca accaatttta tcttacaatt taattttttt
     8521 gcatattcta tatctttttc atctattgaa gttattcctt ctaataataa atctttccaa
     8581 tatactattt ttccataaga taaagtagac aatatagata actttctagc actatcatat
     8641 cctaaaacat ctgcatctgg attaacttct gcaaatccca actcttgtgc ctgttttaaa
     8701 gcgacatcat acggtaaatt ttcatcatac atctttgata atataaaatt acaagtccca
     8761 tttaaaatag cgtttatgct ctttatattg ttgccttcca gtgattctat caatggtttt
     8821 aaaactggaa taccacctgc cacagatgct tcaaacttga tgctaacatt atttaagtta
     8881 gctaattcta taaggtcact acctttttca gccagtaaat ctttattagc tgtcactaca
     8941 tgtatttttt tatttaacgc tcttgttaca taatcaaaag ctggatttaa tcctccaatt
     9001 acttcaacta gtatatcaat atccttgtca aatatatctt ctatatcagt agttatctta
     9061 tcaaagtact tcttatactt gtgtttttct ttatttttaa ctaaaattcc aacaacttct
     9121 atatctcttt ttgaagtatt attttctatt aaatcaatta atccacttcc tactgttcca
     9181 taccctaata ttgctatctt cactgtaaca tacccccatc attttgatta tattaaatta
     9241 tttatatata taaatatacc aataaaaaaa gcttgagtat aaactcaagc catatgacgg
     9301 gtttatactc atctttcagc ataaatgctg caggatttag caccaatgta taattaaata
     9361 tactggttgc cgggcttcac agggccagtc cctctgccac tcttgataag attgctaatt
     9421 taaatttatg tttaatagta taatacaatt tcttccaatt gtcactattt ttttaactta
     9481 tttccctaaa aggatataaa ctattatcta acagctcttt tagttggttt accctttttt
     9541 gtaacattat tatttttagt ctttgctttc gcctgttcat gtcctggaaa aagtatttta
     9601 ggctcttcaa tattcctctt gtaaatagta aatttaaaac caatagcctg cacaaattca
     9661 gctcttaaag cttcacatac tgcattagca gtttctttag tatcaagacc tgcattttct
     9721 aatatagtaa cttttactaa ttctctagtt cttaacatac catctaattg ctctaagaaa
     9781 ctctctgtaa caccttcttt accaatttgt gtagtaggct ttaaagtatt tgccaaagat
     9841 cttaaatacg ccctttgttt tccttttaac atattctcac ctctataatt ctatacttaa
     9901 attatctata atctatattt aaattaattg taaaactcaa attctaattc ataaatttta
     9961 accacatctc catcttctat tcccatttct ctaagcctat caaaaacacc ttgactctcc
    10021 atagcttttt ggaaatattg aagggattcc atatcatcaa aatttacaga atacattatt
    10081 cttctaagtg ctttacctgt aactacatac acaccatctt caatatctat acttaactct
    10141 tcttcagtac caatatcaag ttctggtcta tacatttctt cttctgaaac tagctccaca
    10201 tcttctactt cttttagcat tttagaaaca taggctatta cttcatctat gccttgacga
    10261 gttgctgctg acattttaaa tactgtatat cctctgcctt ctaattcact cttaaacttc
    10321 tcaaacttac tctcatcttc taatatatca aatttatttg caactacaac ttgtggtctc
    10381 tttgaaagct tttcattgta caatttaagc tcatcattta tcttgtcaaa atcttcaata
    10441 gggtctcttc cctctaatcc agatatatct acaatatgta tcaatacctt tgttctctca
    10501 acatgtctta agaactcatg acctaatcct atgccttcag aagcaccctc tataattcca
    10561 ggtatatctg caagtacaaa actatctcca aacttagttt gaacaacacc tagatttggt
    10621 gtaagtgttg taaaatgata gtttgcaatt ttgggtttag ctttagttac aactgataga
    10681 aatgttgatt tacctacatt tggaaatcct aaaagaccaa catctgctat cattttaagt
    10741 tcaagagtta tccatctttc ttctccatct gtaccagatt ttgcaaaagc tggagcttgt
    10801 cttactgcat ttgcaaagtg ttggttacct ttaccaccta taccaccttt tgcaactata
    10861 gctgtatctc cttctttttt taaatctgct aatacaagac ctgtagcctc atctcttatt
    10921 actgtacctg ctggtacttt taatactaaa ttttctccat ttttaccagc tctttttcct
    10981 ttgcttccgt cgccaccatt ttgagcttga tattttttct ggtacttaaa gtccattaga
    11041 gttctaagcc ccaaatcaac ttcaaatatg atgcttgctc cacgaccacc atctccaccg
    11101 tctggaccac cagctggaac gtatttttct cttctaaatg ctaccgaacc atttccacca
    11161 tttccagcct taacaaatat tcttgcctta tctataaaca aattcttcac ctacttaatt
    11221 aaaaactttt aattagcatt accaaaaata ccattataaa ttcatcagta ttataagaaa
    11281 aaggagtgcc tcaacactcc ttttatgtat attactcagc tatagctact ggatatatac
    11341 taactttttt tctcttcttg tcttttcttt cgaatttaac agtaccatca actaatgcaa
    11401 ataaagtatc atcactacct tttccaacat tagttcctgg atgtatttta gttcctcttt
    11461 gtctaactat tatacttcca gcagttacta attgaccatc gaatctctta acacctaatc
    11521 ttttagatat agaatctcta ccatttttag aagaacctac ccctttttta gatgctaata
    11581 attgtaaatt catgtttaac atatggagtc accccctact tttttaaaat ttttatattt
    11641 tttgggtatt cccgtttaat ttctttaacc gctaattgaa agtgatttag taataattgt
    11701 gcgtctttta catgtgtatc tttgactata cactctatat gaccttcttc tcctactact
    11761 ttttcaaatt caatcttttg taatttgtct aaagaattta taactgcaat agcattagat
    11821 gtaactgcag cacatactat atcatagcct atttcagcaa aatcagcatg tccctttaaa
    11881 caaaaacctt gcaacaagaa atcatcatta taataatatt taacttttat cataacttaa
    11941 ccaataacta agcgtttatt ttttctacaa ctatcttagt gtatgattga cgatgtcctt
    12001 gttttcttct gtaatctttt tttgctttat acttgaaaac tataactttc ttagctttac
    12061 cttgttcaac aactttagct tgaacagatg ctccttctac tactggagaa cctaatttta
    12121 attctccatc tttagagcaa gctaatactt cgtttaaagt aacaacatca cctgcgttag
    12181 cttctaattt ttcaacgaat aatacatcac cttcagaaac tttatattgc tttccaccag
    12241 tctttactat agcgtacatt aaatacacct cctcggcctc aaactcgcca gtacaaggta
    12301 cttcatttga agatttaaac ctttactgtg cggcattaca attattaatt ataaaatatt
    12361 tacattttat tgtcaactat tttatcgaaa ataacattgg ttttattgtg ctttaatgta
    12421 ctatttgcgt ttaaagaaat ctttatatta tatttttcac caattttatc aattatatcc
    12481 atataatatt cttttatttg ttcaaaaata aaatcattaa attctaccat tatgtttttg
    12541 taaacagtat gttcacttat tctcataatt tctttttcaa tactatctag tatataatgt
    12601 acagatttta ttgtttgttc accatcacaa catggacaag acattaaata atacttatca
    12661 atggattctt tttctctacg cctagcaact tctactaaac caagttttgt catacctaat
    12721 acttcagctt ttcttttatc tttattaaat tcttcttcta ggatgtgtaa aatctccttt
    12781 tgatgcttct ttttatgcat atcaataaaa tcgattatta ttatcccagc tatgtctcta
    12841 attctaagct gtcttacaat ttcttttgca gcttcaagat tagttttata aacggtttca
    12901 tcaagtttac cagttccaat aaacttaccc gtatttacat ctataaccgt aagagcttct
    12961 gttttttcaa ttattaaata acctccactc tttaaccata cttttttatt taataatttt
    13021 tctattttag attctattct gtataaatca aatacatctc tattttcctc aagacacagt
    13081 ttatcaacat aagtcttatc tatacctctt aaaatagact taagttcaga gtacttatca
    13141 taactattgg taattataga ttcaatatca tcattcacat tatcttttac atatttagtt
    13201 gcaaaatcta aagacttata taataatttc ggacctatac caagtttata ttcttttaaa
    13261 atgttttcat actttaattt caactcttct atatctttct ttatttcatc atgagtgcaa
    13321 ccttgagcct cagtccttat tattaatcct agattttcct tatttactgc cttagtaatt
    13381 ttttttaatc taaatcgttc tttttcatca gtaattttat ttgatattgt agttctatca
    13441 ttggaaggta tatagacaat ataccttcca gaaatactaa tctcagtgtt taattttgcg
    13501 cctttagttc ctatttcttc tttatttatc tgaaccaaaa tatcctgacc agattttata
    13561 gtacttccct tttttaattg taaataagca agtttcttaa atccaatatc tacaaaacaa
    13621 gcttctattc ctggtataac tttttttacg attccacgat atatattgga tacagtcttc
    13681 ttatttaaat tgtcttcaac aaaaagttct gttaatcttt catcttcaag tactgctgtt
    13741 ttctgggacc ctattaggga ctcaataact atctttttca caacttcacc tctcgagctt
    13801 acataggagt cactaattcc ccatcttcta atacatataa atctcttctc aatatatcaa
    13861 catctaatgg gtcaatatca agttcaaaca tctctaatat ctttggtata agtatattag
    13921 tatttaaatt agtttttgag cctgtagcta tagtagcagt aagagtaata tgttcatctt
    13981 ctaaatttaa aacatcaaag gttcttatca taggtcttat atctacttca accatcttac
    14041 cttttttatt cttttttgtt atgattattt ctttattatt cataaagtca attacttttc
    14101 tttttacaaa ctctttcgtc ataggtcttt tcaaatctat attaaacaaa tattctccat
    14161 agtctatcac agaagaaaga gaaggtgttt gtctatcaat ttcttttgct ttgataaaat
    14221 ctatcccatc tggaagttga attgatactt tatttaaaaa ttcctctact gataaatcat
    14281 cttcttctat ttcaatatct acatactcac cttgactttc tgtccctaaa gccaaagcat
    14341 tgccataact cattttaggg tgaggattaa atccttgaga atgtgataag tttatttcag
    14401 ctcttctaaa cgctctttgt aacaatctct gaaggtcaag atgtgatata tatatcatgt
    14461 cgccttcctt tttaaatttt actcttatta tcttactcat attagcaaag cccccttcct
    14521 atatcatgta tatttattcc acaaccattg cagttatgtc tacaatcaga agtgattttt
    14581 tcttttttag cattttcgtc ttctctaatt aagaattttt tactaatacc tacatctata
    14641 tggtcccaag gaaatacctc ttcatagttt ctatttctat gtgcataaaa atctatagaa
    14701 agattattct tttccatagc ttctttccat atatctaaat taaaatgttc tgcccatcca
    14761 tcaaatttag cacctagtct aaatgcatca tatataactt ttcctattct tctatcgcct
    14821 ctagctacta ctgcttccat caaactagtc tttgaatcat gataattgta ttttatattg
    14881 ttatttctta atttatttac taaatgtctc tgcttattaa taacttcttc tgtagtatct
    14941 tgaccatgcc attggaaagg tgtaaatggc tttggtacaa atgtagaagt actcactgta
    15001 acactaaaac ttctctttag ctttcctcca tttacttttc tataaatatc aataacttta
    15061 taagctaatt tagctatacc atctaaatct tcataagtct cagttggtaa accaatcata
    15121 aaatacaact taacactatt ccaccccatc tcaaaagctc tttctgttgc attttccaaa
    15181 tcttcttctg taacaccttt atttataaca tctctaagtc tttgagttcc tgcttctgga
    15241 gcaaaagtaa gtccagattt tcttacttgt tgtattttat cagcaatttc cattgaaaaa
    15301 ttatctaatc taagggatgg aagcgaaatc ccaatattat tagatgcata ttcatctacc
    15361 aaataatcag tcaactctga taacttacta taatcactcg tactaagtga tgataatgat
    15421 atttcatcat atccagtatt cttaactaat ttatctaata tttctttaag tcttttaaca
    15481 ctcttttctc ttattggtct gtatatcata cctgcttgac agaatctaca tcctctagta
    15541 catcctctaa ataattctag tactattcta tcatgaacag tatctatata aggtactatt
    15601 agtttttctg gataatctac agtctcaaca tcttttatta tccttttagt aggattttta
    15661 gtaatccctt ctctattagg agttacactt tgaactgtac catcttcatt gtacttgaca
    15721 tcataaaaac ttggtatgta tactccttct atgctagata ttttatataa aaaatcttct
    15781 ctagtcgttt tatttttctt ccattcttta tactcattta ctacttctaa attaacctct
    15841 tcaccttcac ctaatattac tacatctaca aaatctgcta atggttcgga attataagca
    15901 caaggaccac caaccattat aaatgggtct tccaaagttc tttcttcttt taatattggt
    15961 atattggcta aatctaatat atttaagata ttagtgtatg aaagttcata ttgaagtgta
    16021 aaagtgacaa aatcaaagtt tgttataggc tctcttgact ctaatgcaaa taatggtata
    16081 cttttttctc tcattatatt ttccatatca actgcaggag aatacactct ctcacaaaat
    16141 acatcttcat ccttatttat tacatcatat aatatatgac tccctaagtg actcatacca
    16201 acttcatata aatctggaaa gcaatgtgca tatcttatca attcactatt actagtatct
    16261 ttatgaattg aatttatttc atttccaaga tatctagcag gcttttctac tttttttaag
    16321 atttttttca agtctacttt attcatttat taaaattcct ccaaacaagc tcttta
//
LOCUS       DAGSRM010000087        16213 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.087, whole genome shotgun sequence.
ACCESSION   DAGSRM010000087 DAGSRM010000000
VERSION     DAGSRM010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16213
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.087"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            <1..681
                     /locus_tag="MJE33_002895"
     CDS             <1..681
                     /locus_tag="MJE33_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBY9648818.1"
                     /translation="RILNATISKEPSGRYYVSLCCTDVDIEAFENTNNHIGLDLGIKE
                     FCISSCGEFIENPKYLKKSLNKLAKLQRELSRKTIGSLNRNKARLEVAGLQEHIANQR
                     KDFLQKLSTKLIKENDIICIEDLQVKNMIRNRKLSRLISDVSWSEFIRQLEYKANWHG
                     RQIVKVGKFFASSQICNKCGYKNEEVKDLNIREWICPSCNETHDRDINASINILKEGL
                     RLITTQNK"
     gene            1236..2642
                     /locus_tag="MJE33_002896"
     CDS             1236..2642
                     /locus_tag="MJE33_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBY9648819.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLDFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            2670..3518
                     /locus_tag="MJE33_002897"
     CDS             2670..3518
                     /locus_tag="MJE33_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBY9648820.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            4179..4871
                     /locus_tag="MJE33_002898"
     CDS             4179..4871
                     /locus_tag="MJE33_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY9648821.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLAELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCAKHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            4868..5803
                     /locus_tag="MJE33_002899"
     CDS             4868..5803
                     /locus_tag="MJE33_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY9648822.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIYKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQISVESEIGKYTIFTVSLPLI"
     gene            5951..6874
                     /locus_tag="MJE33_002900"
     CDS             5951..6874
                     /locus_tag="MJE33_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9648823.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            6858..7628
                     /locus_tag="MJE33_002901"
     CDS             6858..7628
                     /locus_tag="MJE33_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9648824.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVQKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPILSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            7638..8420
                     /locus_tag="MJE33_002902"
     CDS             7638..8420
                     /locus_tag="MJE33_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9648825.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVTLVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            8796..9332
                     /locus_tag="MJE33_002903"
     CDS             8796..9332
                     /locus_tag="MJE33_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBY9648826.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            9364..9753
                     /locus_tag="MJE33_002904"
     CDS             9364..9753
                     /locus_tag="MJE33_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBY9648827.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            9790..10176
                     /locus_tag="MJE33_002905"
     CDS             9790..10176
                     /locus_tag="MJE33_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HBY9648828.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            10303..11745
                     /locus_tag="MJE33_002906"
     CDS             10303..11745
                     /locus_tag="MJE33_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBY9648829.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFART
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            12042..12857
                     /locus_tag="MJE33_002907"
     CDS             12042..12857
                     /locus_tag="MJE33_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBY9648830.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTIELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIITGCSHSGICNIVEYAKKVCRDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEIRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
     gene            13459..14643
                     /locus_tag="MJE33_002908"
     CDS             13459..14643
                     /locus_tag="MJE33_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648831.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
     gene            complement(15020..16129)
                     /locus_tag="MJE33_002909"
     CDS             complement(15020..16129)
                     /locus_tag="MJE33_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBY9648832.1"
                     /translation="MDFKSLGISENTINILKKLGITTPTPIQKESIKLIKEGKDVIAE
                     AQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESKDVSILA
                     AYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQMLLMGF
                     KNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLNNIKQEV
                     VETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDHLEEALAIRGYNCQKLHSDIAQS
                     KRERIMKSFRSLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIGRTGRAG
                     EEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
BASE COUNT         5847 a         2009 c         2914 g         5443 t
ORIGIN      
        1 agaatactta atgctactat atctaaagag ccaagtggta gatattatgt atcattatgc
       61 tgtactgatg tagatattga agcatttgaa aatactaata atcatatagg tttagattta
      121 ggaattaaag aattttgtat ctcaagttgt ggagaattta tagaaaatcc taagtatctt
      181 aagaagtcat taaacaagct tgctaaatta caaagagaat tatcgagaaa aacaatcggt
      241 agtttaaata gaaataaagc aagattagaa gttgcaggac ttcaagaaca tatagctaat
      301 caaagaaagg attttttaca aaaactatct actaaattaa taaaagagaa tgatattatt
      361 tgcatagaag atttacaagt aaagaatatg attagaaatc gtaaactatc tcgcttaatt
      421 tctgatgtgt catggtcaga gtttattcgt caattagaat acaaggctaa ttggcatggt
      481 agacaaattg taaaggtggg taaattcttt gcaagttcac aaatatgcaa taaatgtggg
      541 tataaaaacg aggaagttaa ggatttaaat ataagagaat ggatttgtcc tagttgtaat
      601 gaaactcacg atagagatat aaatgcaagt ataaatatat taaaagaagg attaagacta
      661 ataacaactc aaaataaata acttatgtaa gaaccgtagg aactacgggg atagcctgtg
      721 gagaattagt aagacatatt ttagtatgca aaattctgtg aagcaggaac cctgtgactt
      781 tagtcatggg aggttcagtt tgggcaagta gtttcttgta aatctagtta tgtttcaacc
      841 aatgataata taatttcatt agtattagct ggtaattaag ttaatttgac tagagataag
      901 taaattgatt taaaaggaaa taagtaaatc aacaattaaa tacatctaaa agtaagttta
      961 ttgcaatgaa ttgttaaaga aaataattaa tatggagtaa atctattgca atgaagtaac
     1021 aaagaaaata attaatatag agtaagttta ttgtaatgaa ttgttaaaga aaagagttaa
     1081 tatagagtaa gtttattgta atgaattgtt aaagaaaata attaatatag ggtaaatcta
     1141 tctcaatgaa ataacaaaga aaagagttaa tatagagtga acttattgca atgaatcaac
     1201 aaagaaaata atcaatataa aaaggagaaa tatttatgaa tacaggattt ccaaaagact
     1261 ttttatgggg agcatcatct tctgcatttc aagtggaagg agcttgggat aaagacaata
     1321 aaggaaaaac cgtagccgac tataattctt ttaaaaagtc tcatttacag gcagatacta
     1381 aagtcgcatc agacttttac cataattatg aagaagatat tgaattaatg aaagaattag
     1441 ggatgaaaac atatcgtttt tcaatttcat gggcaagaat aataccagat ggtgaaggag
     1501 agataaacca gaaaggttta gacttttata ataagataat agacaaacta atagagtgtg
     1561 atattgagcc attcgtaact ttataccatt ttgaccttcc atttaaatta gtagagaaat
     1621 ataatggttg ggaaagtaga gaaactgtct atgcttttga aagatttgct aaaatatgct
     1681 ttaagcattt tggagatagg gtaaagtatt ggcaacctca taatgaacag aatttaattg
     1741 ttagagtgga ggagagaatt aatatatatg atgaaacaga ttcatggaag atagataaaa
     1801 tgcgtgctca gatggattat aatctttgct tagcacatgc attagcagtc aatgcatgtc
     1861 atgagatgat aaaagaaagt aaaattggag cagcagtatc atcatctgta acatatcctc
     1921 ttacttctaa gccagaagat gtatatgcag ctagaatgaa tgataatttt aaagtgtact
     1981 atatgcttga tatgcatcat tatggagaat atccagggta ttatatgaag tatcttgaaa
     2041 aaagaaatat agttcctcac atggaagatg gggataaaga gatacttaaa aaagctaaaa
     2101 tggattttat agctgtaaat tattatagga caaattgtgc agaagcttta cctgaagatt
     2161 cacaacatcc atttggatta agagaaggaa ctgttgattt tagtatgtat ggactattta
     2221 aaatgtcaat gaatcctaac ttagaggcct ctgagtatgg agcagcaata gacccatcag
     2281 gtcttagagt tgccttaaat gaatattggc aaagatatca cttacctgtt ataataacag
     2341 aaaatggtct aggagctaaa gatatattag aagatggaaa aattcatgat gactatcgta
     2401 tagactattt aagaagtcat ataaatgcat gtaaactagc tattgaagat ggtgtagaga
     2461 tgattggata ttgtccatgg tcatttacag accttttaag ttcttctcaa ggttttaata
     2521 agcgttatgg acttgtttat ataaatagaa ctgaccatga agttttagat ttaaaaagaa
     2581 tcaagaaaga tagtttttac tggtataaag aagttataga gaacaatgga atagtaaaat
     2641 aaacataaat atatattgag ggttttagta tgtacattac aaaggtatta aataattcgt
     2701 tgctacttgc aaaggatgat aatgatgaag aaataatatt gatggggaaa ggcattggcc
     2761 ataattataa ggccgggtct gagttaaata aaaaagatat agaaaagatt tttgttcttc
     2821 atgatgaaaa tataaaaaag agcattatac aattggcaac agaaatagat gcagaatatt
     2881 ttggtatagc tcaaatgata attgcatatg gaatagaaaa atataactta aaacttatga
     2941 accatatata tttggcctta actgaccaca ttgcctttgc agtgagacgt tttaaagaag
     3001 gtataatgat tgaaaatcac tatttgttcg aaatcaagga atttaatcca aaagagtatg
     3061 atataggaaa atatgctatt actgttttta aggaggttct tggtttagac ctaccagaag
     3121 aagagattgg taatatagca ggccatttta taaatgcaca acaagataat ccttatagtg
     3181 atagaaataa gagaagtgct aaaattgtaa atgctatttt gcaaatagtt cattatcact
     3241 tttctattgt atataataaa gagtcatttt attataggag atttgtaatg catctaaagg
     3301 ccttttcaca aagatttctg gctaatgaac catcaagaga aaaaatagat tttatatatg
     3361 agcaagttca aaaaaattgt aaggaagagt atgaatgtgt gaaaaaggta ggagcttata
     3421 tgtataagga atactccaga gaattaccaa gacaagagga gttatatttg atgattcata
     3481 ttcataagat tttaggagaa ttatgcgaaa atagttagaa tatcttaata tgctagtaat
     3541 attataaaaa attaaaaatt aactacactt taaatatgaa taattgcatt acttaagggg
     3601 gttgtcttaa aattgatttt tttaattaaa tttgaagctc aactcctttt ttaaaccttt
     3661 atatcattac aactttaatg tttatacgcc tgtacttcag atgtatatct actttttaca
     3721 tttctcaaca tgcaaatcta ccattttttg cagtatgtta attgaaataa accattgagt
     3781 taaccctaaa tttattctgg acttatagga atagttccaa agatacttta aaatattttc
     3841 tggaataatt gcttatttca catatgtttt attgtgatgt tttgatttaa taaattcttc
     3901 aactctgtat atagtaaatg tattatcatg tttgttttat tcaagcacac ttataatacg
     3961 cttgattttt tcacaatgat gaaatatact aatgggtgtt ataagtataa gaatagcatt
     4021 acacttgaag aagcaagtga taaatgaggt ataaatcctc atatgataaa ctactatttc
     4081 attgaggagc atattctaaa tgatgtaaag atactacaat atgactaaat tcctaatgat
     4141 aaaaaagtct acgaatgagt gtagaaaggg gaactaagat gaataaagtt ttaattatag
     4201 atgatgataa agatctttgt attttgttaa aaaagagtat ctcaattgaa cagattgaag
     4261 caaactattg ccattctggg agagatggac tggctgaact ttcaaaacaa gattatcagt
     4321 tagtagttct agacgtgatg atgccaggta tggatggttt tgaaactatg gaacaaatac
     4381 gaaaatatag cagtattccc atcttaatgc tgacatcaaa aagcgataat tacagcaaag
     4441 tgcatggatt gcgagctggt gcagatgact atctaaccaa accatttgaa attacagaat
     4501 ttactgctcg tgtactgtct ttaatccgac gttacacccg ttttaatcca aaaaatgagg
     4561 agccacagca actttctttc aaaggtttgt tgattgacct tgaccagcat acagtgacta
     4621 ccaatggcga acctatgttg cttccaccca aggaatttgg tgtgcttctt tactgtgcaa
     4681 agcatcaggg taagattttg acaaaacagc agatttatga agccgtgtgg ggtgagccat
     4741 atgtatatga tgacagcaat atcatggcta ttatcagtcg cctaagaaaa aaaattgaac
     4801 tagacccatc atctcccatc tatttgcaaa caataaaagg aattgggtat caattcaaca
     4861 cggaggtatg aaaatggacg aacaaacgat aattcttttc ttactcttgc tgtcgattgg
     4921 tctacttttt ctttccatat ttgctttttc caagcttggg catatttaca agcgcctaaa
     4981 agatatagag gagatactgg ctgatgtaca aattggagac gaaaatcgta aaatactaat
     5041 taagccatgt gatgttatgg ctccattggt ttatcagctt aatgagattg tctacgatta
     5101 tgaaaataag ttactttctc tgaaaaagtc ggataaagct agcaaacaac ttatgacaag
     5161 tttatctcat gatgttcgta caccattaac aactctgatt ggttatctgg atgctgtaca
     5221 cagtggtata gttatcgaac aagaacgaga ggaatacctt gaaatcgcta ggcgtagagc
     5281 ctacgacctc aaggattaca ttgatgttct ttttgactgg tttcggttga actctgatga
     5341 atttacgctg tcaatagaat ctgtagaaat tgcagagttg tcacgcaata ttttgaaaga
     5401 ctggattcct atattccatg aaaagaaact gaattttgaa attgatattc cagaaaatcg
     5461 actaatggta aacctcgacc cagatggata ttcccgtgtg gtcaataatc tggttcaaaa
     5521 tgttcttgct cacagtaagg ctcggcaaat caagattacc atgagtgagg attccagaat
     5581 ggtattactc cgtgttgagg atgatggtgt tgggattgcc agagaaaatt taccacatat
     5641 ttttgagcga ctgtataagt gtgataaagg acgttcagaa aaaggaagtg gtcttggtct
     5701 atctattgtc agtcaaatgg tggagcgaat gggtggtcag atttccgtag aaagtgagat
     5761 tggaaagtat accatattta cagtgagctt accattaata taaaattgtt tttcccccat
     5821 gtgacatgta tacacagggg gattttttgt aagttataaa gttgcaaggt taatacaagg
     5881 ttgttgcaag gttaatgtaa gattgaggtg gtatgataac tttaataaac aaaggaggct
     5941 tttattattt atgaataatt taattattga aacaaaaaat ctcactaagc aatatggtgc
     6001 tcaaaagagc gtttctaatt tgaatattca cgttcagaaa ggtcgcattt acggattgct
     6061 aggaagaaac ggtgcgggta agacgacaac tatgaaaatg ttattgggtc taacaaaacc
     6121 aactactgga gaaattaaaa tatttggtca ggacattcac aataacgaga agaagcttct
     6181 tcctcgaatt ggatgtttga ttgaatcacc agggttttat cctaatctga caggaacaga
     6241 aaatctcaaa atctttgcaa ggcttagagg aataccaaaa aaaaatgcag tagaatcagc
     6301 actggatgta gtcggtttgc caaacaatga caaaaagtta ttttctcaat actctcttgg
     6361 tatgaaacag aggttggcga ttgctcttgc cattatgcat gacccacaac tgttgatttt
     6421 ggacgagcct atcaatgggc ttgatcctat tggtattgca gaagtaagaa catttattcg
     6481 tgatctctgc acagagcgag gtaaaactat tttgatttct agtcacattc tttctgaaat
     6541 tgccttgttg gcagatgaca ttggcattat tgaccatggt attttgttgg aggaagaaag
     6601 tcttgcccaa ttagaagaaa aaaacagtaa atacatacat tttgctgtat ctgatgcagc
     6661 acaggcagca aggcttctgg aaacccaact taacctgaaa aatttcaaag tagcagataa
     6721 ttacaatatt atgctatatg atacagatgt ttctgttgca gaaataaatc gtgctttcat
     6781 tctaaacaac attgaggtat cagaatctca tttatgcgaa gatactcttg aagattactt
     6841 caagaaagta acagggggtg aaggaattgc ttgatataat ttcatgtgaa ttttcaaaac
     6901 tcaagcggtt aaaatttata ctgatttcta ttttaggtgc ttgcttattt ccaattcccg
     6961 caacaatttt gattgcaaag gataacttgc cctttgagca attgttcaaa ttagttgtca
     7021 attttggata ttttttgcta ttaccaattg tattaagtat tgttgctagc caactatttt
     7081 ttatagaaag agataatgat gtattaaaaa atcttgcaac agtgccagtg caaaaaggaa
     7141 aactggctct ggcaaaattg gctgtgttgc tgtttatatc attgttctac tctgttgcag
     7201 ggttaggagc tactatcatt ggaggtttca ttgttggaat agtagagggg gttgctgtaa
     7261 aattaggaat gagcattgct tttggaatta tgttttttgt tactgtactt cctgttgtgg
     7321 tactcattgt atatttcaat cgcagctata ttttttctat tatactttct tttgtatatg
     7381 ctattttcaa ttttagtata tccttaaata ttatcaattt tgaaccaaat aacccaatat
     7441 taagtgtatt acctgctcct gttataatgc gttggtggat ggcattttgg ggagatccca
     7501 ctggcgaata cacggcactt agacagccat atttgctctc aactcctgct tgtgtaggta
     7561 ttttatgttt gataaccatt atagcagtcc tgctgatttg tatgatatac aaaaagcagg
     7621 agaattagga ggtatctatg cttgatataa ttactacaga atttcaaaaa ataaaaagat
     7681 ataatattct atggattggt gttgttgctg ttctgttttc agcactattg gcagtttttc
     7741 aggttgtaag ttcacacggc agtgaccctc taacgtatga gagctttgca aacggagtta
     7801 tctggaataa tttcagtctt agttttccat ttgggattac acttattgga ggatatctta
     7861 ttaaccgtga atatacagac caaaccttaa aaaacatcct aactgttcct atttcattac
     7921 gcaaattgct agtgggaaaa ttaattgcgg ttggaggaat tactgtattg ttaagtcttt
     7981 tcagcttctt atgcacattt attttggggt tgttattttg caatattaat atatctgtta
     8041 cactcgttgt aaaatcctta gttcagattt tgtctgttaa tctgtactgt tatgttgctg
     8101 ttttgccgat tattgcttat tttggtcgta aacaaaatgc atttctgacg ggtgtaggga
     8161 ttgcatttgt atacggtttt tgtggtgtgt ttgttgctgg aagaaatctg acagactttt
     8221 atccaatcac agcaggtctt ggtctagttg gatacaacaa tggtgctggt actgtttatc
     8281 agccacttat tggtgctatg actttgattg taattctaat cttgacaact attctacttg
     8341 tatttacacc taattatgat aaagttatga ccatatctaa gaaaaaaggg ataaagcata
     8401 aaaagtattc tcataaataa cataaatatt gtttctatta tttaagttgc tagacagtga
     8461 aaagaaaaaa tctgtcgtac agtggctata tgctcatgta ttcaagaagc tacagttttg
     8521 atttttcata actgtagctt ctttttgtat ttattaaatc acgactatca taatacttgc
     8581 aataggtata gcgacaagag agagtactgt catatgtgtt ttatatatta cattaagtat
     8641 attctaaaat atatgtttta aataatatat ttgaaaatag gaatatttaa ataaaaaaaa
     8701 taatattata tatagttgac aaaaattggc aaatgatata atatataaat atagaaatag
     8761 taatcattac tattattaaa agggaggaat taattatgaa cttaaaagga actaaaacag
     8821 aaaagaattt aatggcagga tttgcaggag agtcagaagc aagaaataaa tatacatact
     8881 acgcttcaaa agcaagaaaa gaaggttata atcaaatagc tgctatattt gaagaaactg
     8941 ctaacaatga aaaggaacat gctaaattat ggtttaaact tttacatgat ggtatgcctt
     9001 caacagagga aaatttaaag gatgcagcag caggtgaaaa ctatgaatgg actgatatgt
     9061 atgcaaaatt tgctaaagaa gctagagaag aaggatttga caagatagct tatttatttg
     9121 aagctgttgg aaaaatagag aaagagcatg aagaaagata cttaaagctt ttagaaaact
     9181 taaatgaagg aaaaatattt aaaagagatg aagaagttgt ttggcaatgt caaaactgtg
     9241 gtcatgtgta tgtaggtact gaagcaccag aaaaatgccc agtatgtgac catccaaaag
     9301 catattttaa tataaaagct gaaaattatt aaaaattatc attattaaaa aggaatgtta
     9361 actatgaaat tttctaaaca acgagaactg attttaaatg aaatattaaa taatccggtt
     9421 catcctactg cggattacct atatgaaaac ttaaaaaaag ataatccaaa tttaagctta
     9481 ggaactgtgt atagaaattt agctcagcta acggaacatg gctttataag aaaggttagt
     9541 attccaggat atccagatag atttgatggt cgaatagata atcactatca tatcatatgt
     9601 gaagtatgtg gagaggtata cgatttagaa tccgaagttc ttaataattt acaagaatta
     9661 atatctgagg agacagacat aaaaataaca tcttataaca taagttttaa agggatttgt
     9721 aataattgta aaaggtgtag ccaagtaggt tagttttcaa attctaaaaa taattaatga
     9781 ggtgagttta tgtgtagtga acaaaaattt tttatatgtg agacttgtgg aaaccttgta
     9841 ggaatgatac aaagtggtgg tgtacctatt ttctgttgtg gaaaacctat gaaggaactt
     9901 gttcctaata caacagatgc agcagttgaa aaacatgtac ctgtaattga agtggatgga
     9961 aataatgtta ctgttaaagt aagtagcaca actcatccaa tgactaaaga acatcatata
    10021 gcatgggtgt atcttatgac tgaacaaggt ggtcaacgca aatgtctagc agtagatgga
    10081 gaacctgtag ttaaatttgc tttgaatgat gatgataaag tgatttctgc ctatgcttac
    10141 tgtaatttac atggattatg gaaggctgaa ctgtaatatt taaataaatt gagtcaaaaa
    10201 ggaggctata aatgtatttt catacaaata gcctcctata aatttatata aattcaagtt
    10261 ttaattttgt ttaatgtcaa tataatataa ggagaaaata atatgtcaat ttataaatgt
    10321 agtgtttgtg gatatattta tgatgaatct aagaatgata aaacatggga tgaattaagt
    10381 gaagattggg aatgccctgt atgtacaaaa ggtcgtagtt attttggaaa gataagtact
    10441 gtttactatg aagaagatga aaagatagca gaagacatag ttgaagatga atctaaatta
    10501 aatactgaaa aagagggaga tttaaattac ttaagtacat atttaagaag agatgatgaa
    10561 gttgagaaac atatggatat aattcacgaa atggctgtta caggaaaatc tataatagaa
    10621 cctatgagaa ctaaactacc agtaatttct tgggatgata ttttaattat gggtgctcaa
    10681 ttaaatccat tacctttaaa tgagcatgat gaagtaaata caacaacaat aataggaaaa
    10741 aaagcaaaga aacctatgat tatagaaaat ccagtttata tttcccacat gtcatttgga
    10801 gctttgtcta aagaactaaa aatagcttta gcaaagggtg cagcccaaaa taagacagct
    10861 atgtgtagtg gtgaaggagg tatattacca gaagagaagg aagcttcata caagtatatt
    10921 tttgagtatg taccaaataa atatagtgta actgaagaaa atcttaaaaa ttctgatgct
    10981 atagagataa aaattggtca aggaacaaaa cctggtatgg gaggtcatct tcctggcgaa
    11041 aaagtaactg aagaaatagc aaaagtaaga aataagcctg taggtcaaga tgtaataagt
    11101 ccatcttgtt ttgaggaaat acaatccaaa gaggatttaa agaaacttgt agatgaactt
    11161 agagaagttt cagaagggcg tccaataggt gttaaaattt cagcaggtca tattgaaaaa
    11221 gatatggaat ttatagctta tgctaaacct gattttgtaa ctattgatgg tcgtggtgga
    11281 gctactggag caagtccaaa gttgcttaaa gatgcaactt caattccaac aatatttgct
    11341 ctttatcgtg ctagaaaata catagataca catggattag atatagattt ggttataaca
    11401 ggtgggcttc gtatatctac tgattttgct aaggcaattg ctatgggggc tgatgcagtt
    11461 gctattgcaa gttctgctct tatggcagcg gcgtgtcaac aatatcgtat atgtggttct
    11521 ggtaagtgtc ctgtaggagt agctactcag gatgaggaat tgagaaaaag attacatata
    11581 gaaaacagtg ctaatagagt ggcaaacttt ttaaatgtaa gtttagaaga acttaagact
    11641 tttgctagaa cttctggtca taaagatata catgatttaa gtgttgatga tttatatact
    11701 gttaactctg aaatatcaaa ttatacaaac atacagcatg tatagattga agtttgaaac
    11761 tattagattt tagatagcta taaaagtaaa tgataaatat attttataaa agagagtgtc
    11821 ttaaaatgaa ctttttagtt cataagtcac tctttttttt atgaagccaa atatatttat
    11881 cattttagca atgaaaaaga atcttatctc tttattttga gatagattcc ttctatttat
    11941 gaaaattaaa gatattaaat tgtaaaatat tctgtgatgt cgtactatta aactaaagat
    12001 tctgtacttt aaagtaaata aaaatttaag gagtgattat tatggaaaga ttgaagatac
    12061 aagttttagt cgacaataat acttatattg atagatactt tgttggagaa ccagcagtaa
    12121 gctattatat tgagattgat ggaaatagga ttttgtttga ctcagggtat tcagatgtat
    12181 tcatatcaaa cgctgaaaaa ttaaatatag atttaggaaa tttaactcat gttgtttttt
    12241 ctcatgggca taatgaccat acaagaggta tccagttttt agaaaatagg tatgatttat
    12301 ctacaattga acttatttca catcctaact gttttatacc tagaaaacat ggagagaaaa
    12361 gcataggagc tccattttct gctgaggaaa ttaaaaatat ttttatgtat aatcctaagg
    12421 ataagccatt taatctaagc aagaattgtg tgtttttagg tgaaatacct tctataaatg
    12481 attttgaaaa aagagctaaa attggtaaat gtaaaatagg agatttgtgg gaagatgatt
    12541 atgttttaga tgattctgct atagtttgta aaactgataa aggtattttt attattacag
    12601 gatgttctca tagtggaatt tgtaatatag tagagtatgc taaaaaggta tgtagagatg
    12661 atagagttat tggtatacta ggtggttttc atctttttga attgaataat aggttagata
    12721 gtacaataca atatttggat aaagaagaaa taagaatgtt gtacccttgt cactgtgttt
    12781 cttttaaggt aaaagcgaag atgaatgaaa tattgaatat aaatgaagtt ggagtagggc
    12841 tgaccataag tatatagtat ttgaaagtat ataagattta agttgaaggt taaaaagata
    12901 ttttctatag aaaatatctt ttttgttacg tttattttaa gagaaatcaa tatactttta
    12961 tatgaaatat tgtaaaaaaa tttattttta atgagaatat attttcatta ataaacaatt
    13021 ttattatgta ttaataatgt tacaataaat ataaataaat caaagttact tacattacag
    13081 tatatttttt cacaaaatat attgatttat tcttttaaag tttgataatt ccaatgaatg
    13141 taataaaaga agggatttta tactatatga ttattattat aaaataataa agaaaaaatt
    13201 atataataat aaagaaaaaa tgtataatat ttaacattaa aaaagatatt tcataaaatt
    13261 aaaaaacaaa gtgaatttga atactattta aaaaataaaa taaatatata aaaaatattt
    13321 aatataaagg tagaaataag ttgaaaatgt aaagtcattg gaaaaatata gttgaattta
    13381 atgagcaaaa atgttattgt tttatcaata aagtgttaaa ataaaaactt ttaatatcat
    13441 tttagggagg atgatttcat gcaaaaaagt gtaaagaaag caaaagtaac gggctctatg
    13501 ttagccattt ttggtgttgc aagcgttctt tttagttcac atgcaggagg aggatttgct
    13561 acagggaatc aagaaactca gtactatgtg cagtatggat ggacagcacc acttatggca
    13621 attttagcaa tgattatact tactgctacc atgagagaag ttatcataat gtataataat
    13681 aataattgta ggaattataa ggatttattt tgtgagcttt ggagaccata tccaaaactt
    13741 gaaataatat gggagatata ttactatcta atggttttaa tagctgtaag tgctgttata
    13801 gcaggtgcag cagcagtatt tcaaagtata ggagtaaatt attttgtagc tgtatttatt
    13861 attggagttg tacttcttgt ctttactata tttggtgcta tgcttgtatc taaagcagct
    13921 acagcaatga caatagctat tctagtatgt actcttacta tttttattgt tggtataaaa
    13981 gctaaagtac cagaaataac tgaaatatta tcaaatagaa cgagtttcac acctggctat
    14041 atcaagccaa tacttaatac ttttatttat gcaggttttc aatcggttgt tatacctaca
    14101 cttgcaggat gttcaagacc acttcttaag aactcaaaag aagctactaa ggcaatgata
    14161 atttcatttg ttatgaatgc aattgcatta ggacttgctg taactatgct aatgggatgg
    14221 tatagagaaa ttattgctgc tgggcaaaca acactcccta ctctttatgt tgcaggtcaa
    14281 tctggaaatc atactatata cataatttat aatgttgcat tattcctttg tttaatgtct
    14341 acaggagtaa ctactatatt tggtcttgta aatagatttg aagaccataa agcactttct
    14401 ttcctttctt caagaatgaa aagaagggta tttacagctt gtgcaataat ggtagtgtca
    14461 atgcttatat cacttacagg attaagtaat attgttaaat atggatatgg atattgtggt
    14521 tatcttggtc tattcactat agtaattcca ttccttacac taggacatta taagaataag
    14581 aagtttgcaa aggaaaatcc agaagctaaa tggccagcgg aattaaatga aaatgtaaat
    14641 tagatgtagt agcaaaaaat attgctaaag agaatactat agataagaat actatagata
    14701 atatatctgc atgtttatta agattatttg caaaatctaa tattaaacaa ctataaacaa
    14761 gcaatataaa actaagaata aaagataaaa agagggtgtc tcaaaatgga cttttagttt
    14821 ataaggcact cttttttata tgaagtcaag tatattttca ttattttgac aataaaagag
    14881 actaatccta ctttggatag cctctttttt ctttaatata aaatagtata acagcaaagt
    14941 atagattgat acatgttttc tgtagctttc tatatattac agtgtttgtg tggcattggt
    15001 attttaagca atctataaat tataattcta taattcttct actaatttta gattctatag
    15061 ccgtctctat ttcacttaac attcgttcat ttttagggtc aataaaagca catgtatatc
    15121 cttcttcacc agctctacca gttctgccaa tacggtggat gtagtcttct ggtgtttctg
    15181 gtaaatcgta attatatata tgacttactc cagatatatc aagaccacga gaagctacat
    15241 ctgtagctat taagtattgt atatctaaac ttctaaatga tttcattatt ctttctcgtt
    15301 ttgattgagc aatatcacta tgaagttttt gacaattata tcctcttata gcaagagctt
    15361 cttcaagatg gtcaaccctt ctcttagttc tacaaaatat tattgccata aaaggattat
    15421 cttcatctag taccttacat aaggcatcta atttttttct atctgtagtt tctactactt
    15481 cttgttttat attattaaga gtgatttctt ctttttgaat agaaactaca acaggatttt
    15541 ttgtgtatct ataagctaat ttttttactt gagaatttat agttgcagaa aagcatagtg
    15601 tttgcttttt gtttgaagtc tcttttaaaa tagcttccac ttcattttta aatcccataa
    15661 gcaacatttg gtctgcttca tctaaaacaa aagtcttcaa cttactaagg tctattgttt
    15721 ttctatttag atggtctaaa agcctacctg gtgtagctat aatcatatga atatttcctt
    15781 ttagtttctt tatttgagaa ccaatatctt ttcctccata tgctgcaaga atacttacat
    15841 ctttagattc tttaagtttc atagcttctt ctgttatttg aatagctagt tctcttgtag
    15901 gagaaagtat aagtacttgt atatcattta tatctaatga tatattttca aatatcggaa
    15961 gtaaaaaagc tagtgttttt cctgttccag tttgagcttc tgctattaca tcttttcctt
    16021 cttttattag tttaatactt tctttttgta taggtgtagg tgttgttatt cctaattttt
    16081 ttagaatatt tattgtattt tcactaatcc ctaagctttt gaaatccatt gttatcttac
    16141 ctttcatgaa aaattttctt ttttattaac caattaataa tatcatattt gtaagtattt
    16201 ttagtaaatt ttt
//
LOCUS       DAGSRM010000088        16031 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.088, whole genome shotgun sequence.
ACCESSION   DAGSRM010000088 DAGSRM010000000
VERSION     DAGSRM010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16031
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.088"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            65..607
                     /locus_tag="MJE33_002910"
     CDS             65..607
                     /locus_tag="MJE33_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBY9648833.1"
                     /translation="MKICITVGHSILKGGKSTGVNGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDVIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGT
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
     gene            935..2428
                     /locus_tag="MJE33_002911"
     CDS             935..2428
                     /locus_tag="MJE33_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBY9648834.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLGYNIPSRVQREVIPKLLKGQNLV
                     VRSKTGSGKTASFAIPLCENINVDYNNVQALIVVPTRELALQVKDEISDIGRLKKVRC
                     SAIFGKQSIKDQIAELKQRVHIVVATPGRILDHINRGSIKLENVKYLVIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVSLFSATIDEEIKYICEKYMLDYSVINIEENESDTNQKTR
                     QIDDKIIKANGREKYILLKELIYSENPKSVIIFCNTKEKVSKLYNKMSKEGFLIRELH
                     ADLSQERRIFVIKDFKNQKFNILVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKGNSGKAITIVTEKDEKYIENIETYIGYKINELKDIEQSRITHGKVLFEEYSKG
                     VLKEVSKRKKVDKNSYKSKDIKLNIESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGIT
                     NDDIGVIEVQDLCSYVDILNYKGDLILKKYKEIPIKKKMVKVKRDIR"
     gene            2502..2735
                     /locus_tag="MJE33_002912"
     CDS             2502..2735
                     /locus_tag="MJE33_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648835.1"
                     /translation="MKAEDMKRKVSPIDLERILVTGGVIKHEDSIWLLDFWGNKDIAG
                     ILLMPPTRHVILHLNDCCKWKEYFRTKKKFYRS"
     gene            2891..3655
                     /locus_tag="MJE33_002913"
     CDS             2891..3655
                     /locus_tag="MJE33_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBY9648836.1"
                     /translation="MAYFNYNDKQCYYNEIGDGTPLLFLHGNTASSKMFNDIIDFYKD
                     EYKVILIDFVGHGKSQMVDKFSADLWFDEAMQVICFLEAMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWEK
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVLLTGSREDEFVSLISSDFFENTFSLLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
     gene            complement(3940..7119)
                     /locus_tag="MJE33_002914"
     CDS             complement(3940..7119)
                     /locus_tag="MJE33_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBY9648837.1"
                     /translation="MKIDMIYKLIRRNTEPSRWSRGVKYYTHQLVDYIDIDIDDLQNN
                     LLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSKGKSIFICKHIVAIVI
                     KSLKQIKNKKDVPPILIDILGADKDKLSNTNSKTSLDLSKQLLSLLDNTKRERVNLDV
                     LINVDSNKAIAEFKIGIDKMYILKNITEFAYSKLNGYELDFGKKFTYNPNIHYFADED
                     EKLVDIIEEYGTNLSYNFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEYHPK
                     ILNDTLPVRFDFKNIEDKIVLYSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDYSKI
                     YQILSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYFDMI
                     DGNICCDVKYIYDDEDKKFVLPNIQKEATIENKLTSSQFTKENNHYVFKGTDHDLFTF
                     LDLQLESLKEFGDIYYSEKFKLKKIYNASSIQASINQTNQNYLEFNFNISDINPKEYK
                     DILKAFQEKRTFYKLKDGNFIDLSERETKDFFELVENLDIMDKSKNNKIHKNKALYIN
                     DVIRSKNLKFIDGKKELDSICEKFRNFDSINLEIPANLNANLRDYQINGLNWFKVLDY
                     YKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVTPTSLIYNWKNEFEKFAPDIKVLL
                     IHGNKRDREKCFMELENFDVILTTYGTLRNDLEKYSEIKFDYCILDEAQNIKNPVALV
                     TESVKSINAENKFALTGTPMENNLLELWSIFDFIMPGYLYSKAKFQELFINKEDNVKN
                     LKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNKEQKKIYSVYSKDIQDKMKDKNLK
                     KDKIVIFSYLTKLRQLCLDPSIVVKDYNKKSSKIETCLEILRDSINENHKILLFSQFT
                     SVLKNISKELDKYKIKYHYIDGKTNAKERLELVDEFNNSMDKKVFLISLKAGGTGLNL
                     TSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQVIKLIAKGTIEEKIIKLQESKKE
                     LINQFINGELSNEGVLKSLSDEEIINLFN"
     gene            7400..9577
                     /locus_tag="MJE33_002915"
     CDS             7400..9577
                     /locus_tag="MJE33_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBY9648838.1"
                     /translation="MYNKYNNLYLLLGKENVNMDSYRQLKAKIPCKETGIEIKRTLCD
                     ICSPGVHCGIDAYVKDGKVIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKR
                     IGKRGEGKFKPISWDEAYREIAEKLNRVKSEYGAKHVAFFTGYGKWYRPFLQRFASSF
                     GSPNYGTESSSCSTSAQMAGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLI
                     KLKEKGLKFIIVDPRITPTTQKLADLHLRIKPGTDGALALGMANLFIQKNLIDREYIE
                     KYVHGFNEYKEYVKLFDLDTVSRITGVSKDNIVKAVEMLSQGPFCINESAAPIVHHQN
                     GFQNYRAMMSLSAITGNYDRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERI
                     GGERFPLWNEMVTQFQAMDLPRQIEEKTPYEIRALFCHGINARMFPNSGRLFKALNSV
                     DFFVNIDLFMTDTSKYADILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVE
                     ILSDLANAMNIDDDLLKAGYEASIRHMFKNTCVDVDKLKESDLPLKCKDFKPYIVGSY
                     TREGYDTSTSRFELKSTIIEKYKDFGLDALPTYRGFNDNIDDEYTHLLTSGARISNAL
                     HSRLHDIKLSRILKNEASCDISSLDAKELGVISGDYIKITTKIGSLDVKVNVTDRVLY
                     KNIHMYHGYREADINSIICDNFDPYSGFPAYRSCKCKIEKMNR"
     gene            9591..10013
                     /locus_tag="MJE33_002916"
     CDS             9591..10013
                     /locus_tag="MJE33_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBY9648839.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNNIDNGELFRHVFTIEE
                     GENIEYFSLACIHCNEPKCLGVCPKNCFKKEEGFVVLDNQNCIGCKLCEKACEYGALI
                     YGNDGKANKCNGCIERYKCGMDSPCVMVCPTGALKFNK"
     gene            10421..11224
                     /locus_tag="MJE33_002917"
     CDS             10421..11224
                     /locus_tag="MJE33_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBY9648840.1"
                     /translation="MKKLKLIIMFALVSALLVTGCSSGNNKNKEEQKVIRVATSGTYY
                     PFAFKDKDKLKGFEVDFWDEFAKRTNCKVEWVLTDFSGLFGLLEADKADVVSAQLTAT
                     PEREKKYAFSDAYNYSGTNIIVREDDTSIKSIDDLVGKKVGVGTGAVANEILKEKYPN
                     DEIKIVNYSSATLKGNYQDLEMGRLDAVVAQDVEALIAIKEQKLNLKMIEPPIQFGAC
                     SFALQKNEKGEKWTKEINEVIEEMYKDGTLTKISENWLGKDITKEPINE"
     gene            11217..11885
                     /locus_tag="MJE33_002918"
     CDS             11217..11885
                     /locus_tag="MJE33_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBY9648841.1"
                     /translation="MSNFNFEFCLSIFPFLIKALGITILISIVSFFLSILLGVILAFF
                     TQTKSKVLQYITRVYVSFFRGTPLLIQIFFFYFGFPMIFSFMKDCSPYLAIVICLGVN
                     SSAYMAEIIRASIESVDKGQQEACYAFGLSKYQSMVKIILPQAATSAVPPIVSCTLDI
                     IKGSSLGLTIGIQDLMGVAQVEAASSFKYFEIYMITILIYWAVSIILSNIQSKVEKRI
                     RRAY"
     gene            12121..12783
                     /locus_tag="MJE33_002919"
     CDS             12121..12783
                     /locus_tag="MJE33_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBY9648842.1"
                     /translation="MAFNMKYFLELFPTIMKSFNVTVILAISSLVFSLIIGTIVALSS
                     YYKVKVLNQICKVYVSIFRGTPLMPQLFFLYFGLAYMSDFVKNMDPVFATSIVLSLNM
                     GAYMSETIRASILSIDTGQIEAAYAMGLTNLQTMKRIVIPQAVRVALPSLFNNFIDLI
                     KGSSVAFVVGVNDIMGAAKSQGALSFKFFEVYGAVMVIYWCIITPLGWGQKKLEKKIS
                     NY"
     gene            12843..13640
                     /locus_tag="MJE33_002920"
     CDS             12843..13640
                     /locus_tag="MJE33_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBY9648843.1"
                     /translation="MIKRNIIKCLVAVLSLTFVITGCSKKEEVKNEKVITVASSNSAD
                     PYSYVDNGEHKGFEVDMWKEIGKRSGYKIKMEPTGFSSIFGMLDSGKVDVAANFFGMS
                     KERLEKYDASIPYGSDSVCIAVKDGNNEINKLEDLKGKVVAVSEGSQGQQVATSVNKD
                     NLFQEKVYGDGTSGLQDLDLGRVSAWIDAELTIVGDAKKANMKVRVLDKKLSATNIAY
                     FFKKNDDKSKTIKEDVNKAIEEMLADGTVKKISEKWFGMDVSADIQK"
     gene            13758..14948
                     /locus_tag="MJE33_002921"
     CDS             13758..14948
                     /locus_tag="MJE33_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBY9648844.1"
                     /translation="MEVLQMIETKIKSIALKNREKIIKIRRQIHSNPELAFKEYKTSK
                     LIKEELNKLNIEYINIAGTGVLATIKGKNNGGKTILLRADMDALPIKEENDLEFKSIN
                     DNMHACGHDAHVSWLLGTAMILNDIKEELNGNVKLLFQPGEEKGGSDIVIKENVLEGV
                     DALATGHCWPTIESGKIGIARNCAMAATNTFEITIIGKGGHGAEPHNCIDPIAVGNAV
                     YSSIQQIVSRKIDPVVPVVVSVCSFNSGVSKNIIPDVCTLQGTIRAISKEQVIEVSKI
                     LEYIVKNVCKAHGADCKFEKSTGGDAVINDKNMIEIGRESACKILGAENVEIIDFPAM
                     TGEDFAIYMKEKPGLFMYIGVGDKEKNINYRLHSNKFNIDEKCLSIAPSLFSQLTVDY
                     LCQL"
     gene            15057..15806
                     /locus_tag="MJE33_002922"
     CDS             15057..15806
                     /locus_tag="MJE33_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBY9648845.1"
                     /translation="MIKVENLHKSFKGTEVLKGINLEISQSEVVAILGSSGTGKSTLL
                     RCLNYLVEPTKGKIMIGDVKVDVEKATKKDITNLRKHTSMVFQNYNLFKNKTALENVM
                     EALIVVHKKSKKEAREISLKLIDKVGMLDRKDFYPSKLSGGQQQRIGIARAMAVNPNV
                     ILFDEPTSALDPELVGEVLSVIKSLAKEGITMLIVTHEINFAKEVADKIIFMDNGVIA
                     EEGTPDEIFNNPKNERTAKFLNIIRKEEVFS"
BASE COUNT         6231 a         1683 c         2652 g         5465 t
ORIGIN      
        1 taataacttt aaaattatat tactattata taaaaattgg cttaaaagaa ggtgaaaaaa
       61 gtaaatgaaa atatgcataa cagtaggtca tagtatacta aaaggtggta aaagtacagg
      121 tgtaaatggt atagttgatg aatacaatta taataaaaaa ttagctccta tgttagctga
      181 gatgcttata agtcaaggta atacagtaga tgtaataatt tgtccagaaa ggcactttat
      241 gtcagaaaga gaagaatttt tctatagagt tccaaaagta aacagtggta aatacgattt
      301 attagtagag ctacatttaa ataaagctga tggaacacaa tgtggtacag aagtgcttta
      361 ctatggcact gaaggattag aatatgctaa aagagtatct aatagacttg gagaattatt
      421 tgaaaataga ggagctaaaa aaagagaaaa tttatatata ttgagaaata caaatccagt
      481 tgcaatacag attgaaagtt ttttctgtga taatgtgaat gattgtaaca aagcaaatga
      541 atcaggttat gattatatag cacgattgat tacagaaggt atattgaata aagatataaa
      601 tatgtgagat tatggtgatt taggtattga ttaatacctt ttaatattag atataaataa
      661 aaatacaatg tattagtata atttaaactt tcaaaagata gattagtttg attattgaca
      721 agagatacct ccttgaaaat attgatttat cagtatattt aaggaggtat ttaaatataa
      781 aaatttaatt aatttgctac taaactatag gtttatagag tatatacttt tatcttcagt
      841 ctaataaata atataaaaat agtagaacct tgtattataa taatataatg tagaaaaata
      901 tatagataag ataaatataa taaggtggta atatatgaaa tatacatttg aaaaatttaa
      961 gttaaatgaa aagatactta agtcattgaa gagtttagga tataatatac cttctagagt
     1021 acaaagagaa gttattccaa aattattaaa aggtcaaaac ttagtagtta ggtcaaaaac
     1081 aggaagtgga aagacagcaa gttttgcaat acctttatgc gagaatataa atgtagatta
     1141 caataatgtt caagctctta tagtggttcc aacaagagaa ttagccttgc aagtaaaaga
     1201 tgagatatca gatataggga gactaaagaa ggtgagatgt agtgctatat ttggcaaaca
     1261 atctataaaa gaccaaatag ctgaattgaa acaaagggtt catattgttg tagctactcc
     1321 aggcagaata cttgaccata taaatagagg ttctattaag ttggaaaatg taaagtattt
     1381 agtaatagat gaggcagata aaatgtttaa taaaggtttt gttgaacaaa tggaaaaaat
     1441 attattaaat ttacctaagg agaaaatagt aagtcttttt tcagctacaa tagatgaaga
     1501 gataaaatat atatgtgaaa aatatatgct agattacagt gttataaata tagaagaaaa
     1561 tgagagcgat actaatcaaa aaacaagaca aatagatgac aaaataatca aggcaaatgg
     1621 aagagagaag tacatccttt taaaagaatt aatatacagc gaaaatccaa agagtgtgat
     1681 aatattttgt aataccaaag agaaagtatc taaattatat aataagatga gtaaagaagg
     1741 ttttttaata agagagttac atgcagattt atcacaagaa agaagaattt ttgtaattaa
     1801 ggattttaaa aaccaaaaat ttaatatatt agttagtagt gatgtagcgt ccagaggaat
     1861 acatattgat gatatttctt tagtaataaa ctatgatgtg ccacaagata aagaaaacta
     1921 tatacataga ataggtagaa ctgggagaaa aggaaatagt ggaaaggcaa ttactatagt
     1981 aactgaaaag gatgaaaaat atatagagaa tatagaaacg tatataggat ataaaataaa
     2041 tgaattaaaa gatatagaac aaagtagaat tacacatggc aaagttttgt ttgaagaata
     2101 ttcaaaagga gtactaaaag aagtatcaaa gagaaaaaaa gttgataaaa attcttacaa
     2161 aagtaaagat ataaaactaa atatagaaag tgaagttgtt aaattatatt taaatgctgg
     2221 taagaagaag aaaataagag ttttagatat agttggtgca tttagtaata taaaaggtat
     2281 aactaatgat gatataggtg taattgaagt tcaagattta tgttcatatg tagatatatt
     2341 aaattataag ggagatttga tattaaaaaa atataaggag atacctataa agaaaaaaat
     2401 ggttaaagtg aaaagagata tacgataatt tagtcaatat tgatgaattg ctttgttatc
     2461 gaaatgaagt tgcaaaaaac acaatagaag ttgttaagga tttgaaagct gaagatatga
     2521 aacgaaaggt ctctccaatt gacttagaaa ggattttggt gactggagga gtaataaagc
     2581 atgaagattc tatttggttg ttggactttt gggggaacaa agatattgct ggaattttgc
     2641 ttatgcctcc tactcgtcat gttatacttc atttgaatga ttgttgtaaa tggaaggaat
     2701 attttcgtac aaaaaagaaa ttttacagaa gttaaattat aataaattag acaaacaata
     2761 gataaattag caaggaatgt tgattttatc tatattctaa gttatactat ttttaagtgg
     2821 ctaagggaat agtcatttta aatataagta ataaatatat acctataggg atattaaagg
     2881 ggggaaatat atggcatatt ttaactataa cgacaaacag tgctactata atgaaattgg
     2941 ggatggtaca ccattacttt ttttacatgg aaatacagcc tcttccaaga tgtttaatga
     3001 tataatcgat ttttataaag atgaatataa agtaatatta attgattttg taggtcatgg
     3061 aaaatctcaa atggtcgata aattttctgc tgacttatgg tttgatgagg ctatgcaagt
     3121 gatatgtttt ttagaagcaa tgaactataa aaaagtaaac ataattggta gtagtggagg
     3181 cgctcttgtt gcattaaatg ttgcacttga gagacctgat ttagtgaata aggtaattgc
     3241 agatagtttt gaaggagaag ttcctctaga gtcatttgta caaaatgtga aaatagagcg
     3301 tgaagcatct aagcaagatg atggtgcaaa agctttttat atttataatc aaggagaaaa
     3361 ctgggaaaaa gttgtagata atgatacaga agctattttt gaacattata aaactatagg
     3421 aaagtttttt cataagcctc tagaaactat gcaaccagag gtgcttttaa caggaagtag
     3481 agaagatgag tttgtatctc taatatctag tgattttttt gaaaatactt tttcattact
     3541 tcttgaaaag attaaaaatg gtaaaatgta tttgtttgat aaaggtggtc atcctgcaat
     3601 tttatcgaat ggattagatt tttcaaatgt agcaaagaaa tttttagagg aataatttaa
     3661 ccaaaattta caataatgaa aactgtaaaa tggattgcaa aacttacaaa agtgagtgta
     3721 catatacttc attattatga tgaaatagta ttgttaaaac aaaatattct aatagtcaag
     3781 ttattacatt tgtatatgca atatgtatag aaagagatat cattagattg taaagttggg
     3841 attaaataac aatattaaat tttatttatt cagatatttt agcttaacta tataaatgtt
     3901 agaacaaatt tatatagtta agctgtagga ttaaataatt tagttaaata agtttattat
     3961 ctcctcatca gatagacttt ttaatacacc ttcattagaa agttctccat ttataaattg
     4021 atttataagc tcttttttac tttcttgtaa ttttattatt ttttcttcta tagtcccttt
     4081 agcaatcaat tttataactt gtactgaatt tttttgacca aatctatgag ctctatcact
     4141 agcttgattt tctactgatg gattccacca tgggtcaaag tgaattacca tgtcagcaga
     4201 tgtaagattt aaaccagttc cgcctgcttt taaagatatt aaaaaaactt ttttatccat
     4261 actattattg aactcatcta caagttcaag tctttctttg gcattagttt taccatctat
     4321 ataatggtat ttaattttat acttatcaag ttccttactt atgttcttaa gcacagaagt
     4381 aaattgtgaa aacaataata ttttgtggtt ttcgtttata ctatccctaa gtatttctaa
     4441 acatgtttca atttttgaac tttttttatt gtaatctttt actactatac ttgggtctaa
     4501 acaaagttgt cttagtttag ttaaataaga aaatataact attttatctt tctttaaatt
     4561 tttatccttc attttatctt gtatatcttt tgaatataca ctatatatct ttttctgctc
     4621 tttattcaat tcaacaaaaa agtttttttc aatcttatct ggaagttctt tcataacttg
     4681 ttttttactt cttctaagaa taaaaggttt tatcaatttt ttcaggtttt taacattatc
     4741 ttctttattt ataaacaatt cctgaaactt tgctttacta tatagatagc caggcattat
     4801 aaaatcaaat atagaccata attctagtag attattttcc ataggtgtac cagtgagtgc
     4861 aaatttattt tctgcattta tactttttac agactcagtt actaaagcta ctggattttt
     4921 tatattttga gcttcgtcta atatacaata atcaaacttt atttcactat atttttctaa
     4981 atcatttcta agagtaccat atgttgtaag tattacatca aaattttcta attccataaa
     5041 acatttttct ctatctcttt tatttccatg tatcaataaa acttttatat ctggagcaaa
     5101 cttttcaaat tcatttttcc aattataaat gagtgatgtc ggtgtaacaa taagactttt
     5161 tttattagat aatgataata aaaatgcaat agtctgtatt gttttaccaa gacccatttc
     5221 atcagctaat ataccaccaa atttataata atctaaaact ttaaaccaat tgaggccatt
     5281 tatttgatag tcacgaaggt ttgcatttaa atttgcggga atttctaaat ttatgctatc
     5341 aaaatttcta aatttttcac atatactgtc tagttctttt ttaccatcta taaactttaa
     5401 atttttacta cgtattacat catttatata tagagcttta tttttatgaa ttttattatt
     5461 ttttgattta tccattatat ctagattttc aactaattca aaaaaatctt ttgtttctct
     5521 ttctgataaa tctataaagt ttccatcttt aagtttataa aaagtacgtt tttcttgaaa
     5581 agctttcaaa atatccttgt actcttttgg atttatatca gatatattaa aattaaattc
     5641 taggtaattc tggttagtct gattgattga agcttgaatt gatgaagcgt tatatatttt
     5701 tttcaattta aacttctcag aataatatat atctccaaat tcctttagag attctaattg
     5761 taaatctaaa aatgtaaata ggtcatggtc agttccttta aacacataat gattattttc
     5821 ctttgtaaat tgtgaagaag taagtttatt ttcaatagta gcttcctttt gtatgttggg
     5881 gagcacaaat tttttatctt catcatcata tatatacttt acatcacaac atatgttgcc
     5941 atcaatcata tcaaagtaga actcagattt aaaatctttt gttatattat tttttatgtt
     6001 atcatctaaa tgaacttctt gagatataga ttttaatcta ggaactagca atcctaaaac
     6061 atccttagaa tcctctttat caaattttaa ctctttagtc tttgataata tttgatatat
     6121 ttttgaataa tcatgagctt ttttatgtga taataaatag atattgccat catataaaag
     6181 tacatcattt tttttagtaa gtggaacagg tagtgaatct ttagaatata agactatttt
     6241 atcttctata tttttaaaat caaaccttac aggtagagta tcatttaata tctttggatg
     6301 atattcctct ttagaaaaaa taagggtaaa atccttgtac ttaatagcat ttaaaaattg
     6361 ttttaaagag cttgcattta tctccataaa tttttttgaa tgtgatgaag aaaagttgta
     6421 tgaaagattt gtcccatatt cttctataat atctactaac ttctcatctt catcagcaaa
     6481 ataatgaatg ttggggttgt aagtaaattt cttcccaaaa tctaattcat atccattaag
     6541 ttttgaatat gcaaattcag taatattttt taaaatgtac attttatcaa ttcctatttt
     6601 aaattcagca attgctttat ttgaatccac atttatcaat acatctaaat taaccctttc
     6661 tcttttagta ttgtctaaca aagaaagtaa ttgcttgctt aaatctaagc tggttttaga
     6721 atttgtatta gaaagcttat ccttatctgc acctaatata tctattagaa taggaggaac
     6781 atctttttta ttttttattt gttttaagct ttttatcaca atagcaacta tatgcttaca
     6841 tataaaaata cttttgccct ttgattgaca gtcagcgcaa ctacaatcat tttcaattat
     6901 cttaaagtgc tttatatcta tagttattga attaaaataa aagctataat tatcttcaga
     6961 attgactcca ccacgaaatg tcaacaggtt attttgtagg tcatcaatat ctatgtctat
     7021 ataatcaact aattgatgag tataatattt tacacctctt gaccatcttg atggttctgt
     7081 atttcttctt attagtttat atatcatatc tattttcata ttattcacct tacctcactg
     7141 tatatattat aatctaaatg cattcaattt acactctaaa tttaagacta aaattaaggc
     7201 agatatgaat agcttttatc tatcacaata tatgtgtaaa taacaattta ctattagatt
     7261 tttattgaat tatcttataa actatgaatg aaaaataatt atataaatag attattaata
     7321 aatataaaat cttattatta aaaatattct tagaaattac actatataaa atataaagta
     7381 tgatatttga ataaaaagaa tgtataataa gtataataat ttgtatttat tattgggaaa
     7441 ggaaaatgtt aatatggata gttatagaca acttaaagct aaaataccct gtaaggaaac
     7501 aggtatagaa ataaagagga ctttatgtga tatttgttct ccaggagtac attgtggaat
     7561 tgatgcatat gttaaagatg gtaaagtaat aaaaatagaa ggtacaaaag aacatccaat
     7621 gaataaaggg ttgttatgta ccaaaggtct ttgcaataga gaatatatct atagagaaga
     7681 tagaataaaa acacctctaa aaagaattgg aaaaagaggt gaaggaaaat ttaaaccaat
     7741 atcatgggat gaagcttata gagaaatagc agaaaaatta aatagagtaa aatctgagta
     7801 tggagcaaag catgtagctt tttttacggg ttatggaaag tggtatagac cttttttaca
     7861 aaggtttgca tcttcatttg gttcacctaa ttatggtaca gaatctagtt catgttcaac
     7921 atcagcacag atggctggaa atgttgctac tggaagaaac tttacaatgc aagatatgga
     7981 gaatgcaaaa acttatttag ggtggggatt taatccatac tactctaaat ttttaaatag
     8041 tgtgaatcta ataaaattaa aagaaaaagg tctaaaattt ataattgtag accctagaat
     8101 aacaccgaca actcaaaaac tagcagattt acatttaaga attaaacctg gaactgatgg
     8161 agctttagct cttggaatgg cgaatctatt tatacaaaag aatttaatag atagagaata
     8221 catagaaaag tatgtacatg gatttaatga atataaagaa tatgtaaaat tatttgattt
     8281 agatactgta agtagaatta caggagtttc taaagataat atagtaaaag ctgttgagat
     8341 gttgtcacaa ggtccatttt gtataaatga aagtgcagct ccaatagttc accatcaaaa
     8401 tggatttcaa aattatagag caatgatgtc tttatctgct ataacaggta attatgatag
     8461 aataggtgga aacataccaa aacaagatta ctttgctggt aagataggtg gattccaaac
     8521 actagaaaaa gagtttgtaa aagaagttct acctaaaaat ataaatgaaa gaattggtgg
     8581 agaaagattt cctctatgga atgaaatggt gactcaattt caagctatgg atttacctag
     8641 gcaaatagaa gaaaagacac catatgaaat aagagctttg ttttgtcatg ggataaatgc
     8701 tagaatgttt cctaattcag ggagactttt taaagcgttg aatagtgtag atttttttgt
     8761 gaatatagat ttatttatga cagatacatc aaaatatgca gatatacttt tacctgcatg
     8821 tacatcattt gagagagaag aatgtaagag ttatccagga gggtatatta catataccaa
     8881 aaaagttatt gataaattat acgattcaaa atcagatgta gaaatacttt cagatttagc
     8941 aaatgctatg aatatagatg atgatttatt aaaagcaggt tatgaagcaa gtataagaca
     9001 tatgtttaaa aatacatgtg tagatgtaga taaacttaaa gaaagtgatt taccattaaa
     9061 atgtaaagat tttaagccat atatagtagg ttcatataca agagaagggt atgatactag
     9121 cacttctagg tttgaattaa aatctacgat tattgaaaaa tataaagatt ttggactgga
     9181 tgcactacca acatatagag gatttaatga taatatagat gatgaatata ctcatttatt
     9241 gacatcagga gcaagaattt caaatgcact acattcaaga cttcatgaca tcaaactttc
     9301 aagaatatta aaaaatgaag ctagttgtga tatttctagc ttagatgcaa aagaattggg
     9361 tgttatcagt ggagattata taaaaataac tactaagata ggaagtttgg atgtaaaagt
     9421 caatgttact gatagagtct tatataagaa tatacatatg tatcatggat atagagaagc
     9481 tgatataaat agtattattt gtgataattt tgacccttat agtggattcc ctgcgtatag
     9541 aagttgtaag tgtaagattg aaaaaatgaa tagataaatg gagagatata atgaaaagtt
     9601 tatttaaatt taataaagaa ttatgtacag catgtggagc atgtttgctt gcatgtatag
     9661 accaaaatga taataatata gacaatggtg aactttttag acatgtattt acaatagagg
     9721 aaggtgaaaa tatagaatat ttttcattag catgtataca ttgtaatgag ccaaaatgtt
     9781 taggtgtatg tcctaaaaat tgttttaaaa aagaagaagg attcgttgta ttagacaatc
     9841 aaaactgtat agggtgtaaa ctttgtgaaa aagcttgtga atatggagcc ttaatttatg
     9901 gaaatgatgg taaggcaaat aagtgtaatg gttgtattga gagatataag tgtggtatgg
     9961 attcaccttg tgtaatggta tgtcctacag gagcattaaa atttaataaa taaattaaat
    10021 atagagaatg acttatactt aggtataaga tatttttaca aaaagctgta ttgagtttgt
    10081 tataatgaaa actcaatata gcttttttat ggatatagtt ggatactata atgtgattct
    10141 tagtaatagt tttttgctat tggacaaaga ctgtaaaaca tattaaactt atgttgttca
    10201 aagtcgaagt ataatataat aactagaaaa ttggtttata gtgctataaa agcaaattta
    10261 gtaaggtata aaatcagagg caatccttta ctataatatc aatatttggg agttctgtta
    10321 ttgcagagct attaaacaat tataatggta caagtgattc aacctttgaa aatgtattat
    10381 ataaaaaatt aaaataagat taaaaaggag agaataaatt atgaaaaaac ttaaattaat
    10441 tattatgttt gccttggtat cagcactgtt agttacagga tgttcatcag gaaataataa
    10501 aaataaagaa gaacaaaaag ttataagagt tgcaacatca ggaacatatt acccttttgc
    10561 ttttaaagat aaagataaac ttaaagggtt tgaagtagat ttttgggatg aatttgctaa
    10621 acgaactaat tgtaaagttg aatgggtatt aactgatttt agtggattat ttggattatt
    10681 agaagctgat aaagcagatg ttgtatctgc tcaacttact gcaacacctg aaagagagaa
    10741 aaaatatgct ttttcagatg cttataatta tagtggaaca aacattattg ttagagaaga
    10801 tgatactagt ataaaatcaa tagatgattt agttggcaag aaagtaggtg tgggtactgg
    10861 agcagtagca aatgaaatat taaaagaaaa atacccaaat gatgaaataa aaatagtaaa
    10921 ttattcaagt gcgactctta aagggaatta tcaagaccta gaaatgggaa gattagatgc
    10981 agtagttgcg caggatgttg aggctttaat agctataaaa gaacaaaaat taaatcttaa
    11041 aatgatagaa cctccaattc aatttggtgc ctgttctttt gcgcttcaaa aaaatgaaaa
    11101 aggtgaaaaa tggacaaaag aaattaacga agtaattgaa gagatgtata aagatgggac
    11161 tttgactaaa atatctgaaa actggctagg aaaagacatt actaaggagc ctattaatga
    11221 gtaattttaa ttttgaattt tgtctaagta tatttccatt tttgattaaa gcattgggaa
    11281 taacaatact aatatctatt gtttcatttt ttttatcaat attattaggt gtaatacttg
    11341 cctttttcac acagacaaaa tcaaaagttt tacaatacat aacaagagtg tatgtttcat
    11401 tctttagagg aacaccactt ttaattcaaa tatttttctt ttattttgga tttccaatga
    11461 tattttcttt tatgaaagat tgttcacctt atttagcaat agtaatttgt ttaggtgtaa
    11521 attcatctgc atatatggct gaaattatac gagcatctat tgagagtgtt gataaaggac
    11581 aacaggaagc atgttatgct tttgggttaa gtaaatatca gtcaatggtt aaaataattc
    11641 tacctcaagc agctacatct gcagtacctc ctattgtaag ctgtacactg gacataatta
    11701 aaggttcttc tttaggttta actataggaa tacaagattt aatgggagta gctcaagtag
    11761 aggctgcttc aagttttaaa tattttgaga tatatatgat aacgatatta atttactggg
    11821 cagtatctat aatattgagt aatatacaaa gcaaagttga aaaaagaata agaagagcat
    11881 attagatttc aatatttaaa aatgggatga tatagatata ttattacttg taaataaatt
    11941 tttaatttta gaaaaataaa cttagtaatc taaattattg agaaaatata gtatcaaaat
    12001 ggactgtata atcaagttaa aaaatagttt ttctattgaa aaaataaata aaattctatt
    12061 gtaatttaat atgcgataaa gttttataaa aaacaacatt aagctagggg gacttatttt
    12121 atggctttta atatgaaata ttttttagaa ttatttccaa caataatgaa atcgttcaat
    12181 gtaactgtga ttttggcaat aagttcatta gtattttcat taattatagg tacaatagta
    12241 gcgttatcat cttattacaa agtgaaagta ttgaatcaaa tatgtaaagt atatgtatct
    12301 atttttagag gtacacctct tatgccacag ttattctttt tatattttgg actagcatat
    12361 atgagtgatt ttgttaaaaa tatggaccca gtatttgcaa catctatagt acttagttta
    12421 aatatgggag cttatatgtc tgaaacgata agggcatcta tactttcaat tgatacaggt
    12481 cagatagaag cagcatatgc aatgggatta acaaatttac aaacaatgaa aaggatagtg
    12541 ataccacagg ccgtaagagt tgcactgcca tcattattca ataattttat agatttaatt
    12601 aaagggtctt ctgtagcttt tgttgttgga gtaaatgata taatgggtgc tgccaaatca
    12661 caaggagcgt tgagttttaa gttttttgaa gtgtatggag cagttatggt aatttactgg
    12721 tgtataataa ctcctttagg ttgggggcaa aaaaaattag aaaagaaaat aagcaattat
    12781 taagttatta agaaaataca taaaagtatt tttatataaa aaatggtgtg aggagaagag
    12841 atatgataaa aagaaatatt ataaaatgtt tagtggcagt tttatcgtta acgtttgtaa
    12901 ttactggttg ttcaaaaaaa gaggaagtaa agaatgaaaa agttataact gtagcttcat
    12961 ctaattcagc tgacccatat tcttatgttg ataatggtga gcataagggt tttgaagttg
    13021 acatgtggaa agagattggt aaaagaagtg gatataagat aaaaatggaa ccaacaggtt
    13081 ttagttctat atttggtatg ttagattctg gtaaagttga tgtggcagcc aacttctttg
    13141 gaatgtctaa agaaagatta gaaaaatacg atgcttctat accttatggt tctgattctg
    13201 tgtgtatagc tgtaaaagat ggaaataacg aaattaataa gcttgaagat ttaaagggaa
    13261 aagttgtagc agtttcagaa ggttctcaag gtcaacaggt tgcaactagt gttaataaag
    13321 ataatttgtt tcaagaaaag gtttacggtg atggaactag tggtcttcaa gatttagatt
    13381 tgggaagagt tagtgcatgg attgatgcag agcttactat tgtgggagac gctaaaaaag
    13441 ctaatatgaa agtaagagta ttagataaaa aattatcagc tactaatata gcatacttct
    13501 ttaagaaaaa cgatgataaa tctaaaacga taaaagaaga tgtaaacaaa gcaatagaag
    13561 aaatgcttgc agatggaaca gtgaaaaaaa tatctgaaaa atggtttgga atggatgtaa
    13621 gtgctgatat acaaaaataa atactttaaa attaaaatgt aaaactgtga taactataga
    13681 taagtatact aaatttatct atagttatct gtgtattaca atgtatttag attagtcttt
    13741 ataaatcaaa atgagttatg gaggtattgc aaatgattga aactaaaata aaatccattg
    13801 cactaaaaaa tagagaaaaa attataaaaa taagacgaca gatacacagt aatccagagt
    13861 tagcatttaa agaatataaa acatctaaac ttataaaaga agagctaaat aaacttaata
    13921 tagaatacat aaatattgct ggtacaggag tattagctac tataaaagga aaaaataatg
    13981 gtggaaaaac tatattactc agagcggata tggatgctct tccaatcaaa gaggaaaatg
    14041 atttagaatt taaatcaata aatgataata tgcatgcttg tgggcatgat gcacatgttt
    14101 catggttgtt aggaacagca atgattttaa atgacataaa agaagaattg aatggaaatg
    14161 taaaattact atttcaacct ggcgaagaaa aaggtggttc tgatattgta attaaagaaa
    14221 atgtacttga aggtgtggat gcattagcga ctggtcattg ttggccaact attgagtcag
    14281 gaaaaattgg aatcgctaga aattgtgcta tggcagcgac taatactttt gaaataacaa
    14341 taattggcaa gggtgggcat ggagcagagc ctcataattg tatagaccca attgcagttg
    14401 gaaatgcagt atattcatcc attcaacaga tagtttcaag aaaaattgac ccagttgtac
    14461 ctgtagtagt gtctgtttgt tcttttaatt ctggtgtttc taaaaatata ataccagatg
    14521 tatgtacact tcaagggact attagagcga taagcaagga acaagtaatt gaagtaagta
    14581 aaattctaga atatatagta aaaaatgtat gtaaagcaca tggagcagat tgcaaatttg
    14641 aaaagagtac aggaggagat gctgttataa atgacaaaaa catgatagag ataggaagag
    14701 aaagtgcttg caaaatactt ggagctgaaa atgttgaaat aattgatttt cctgcaatga
    14761 caggtgagga ttttgctatt tatatgaaag aaaaacctgg tttatttatg tatataggtg
    14821 taggagataa agaaaaaaat ataaattaca gattgcatag taacaagttt aatatagatg
    14881 aaaaatgtct tagtatagca ccttcattat tttcacaact tacagtagat tatttatgtc
    14941 aactatagag tacattcata aaaatatatt tagcctattt ttaaatttaa tgctagtata
    15001 ttaattttga tgtagaacat attatttatt atatttattt aaaaaggtgg ataacaatga
    15061 taaaagtaga aaatttacat aaaagtttta aagggacaga agttcttaaa ggtataaatt
    15121 tagagataag tcaaagtgaa gtagtagcta tacttggatc atctggaaca ggtaaatcta
    15181 cgttacttag atgtttaaat tatttagttg agcctacaaa aggaaagata atgattggtg
    15241 atgtaaaagt agatgtagaa aaagcaacaa aaaaagatat aaccaattta agaaaacata
    15301 catctatggt gtttcaaaat tataacttat ttaaaaataa aacagcatta gaaaatgtaa
    15361 tggaggcatt gatagtagtt cataaaaaga gtaagaaaga agctagagaa atatctttaa
    15421 aattaataga taaagtaggt atgttggata ggaaagattt ttatccatca aagttgtctg
    15481 ggggacagca acaaaggata ggtatagcga gagcgatggc agtaaatcca aatgtaatat
    15541 tatttgatga gcctacatct gcacttgacc cagaacttgt tggagaagtt ttaagcgtta
    15601 taaaatctct tgcaaaagaa ggtataacga tgcttattgt aactcacgaa ataaattttg
    15661 caaaagaagt tgctgataaa attatattta tggataatgg tgtaatagct gaagaaggaa
    15721 ctcctgatga aatatttaat aatcctaaaa atgaaagaac agcaaaattt ttaaatatca
    15781 ttaggaaaga agaagtattt agttaaaagt gtataaatta agcattagaa gatatgtaag
    15841 atacccccat taaatttata agttagataa taaatttaat gggagtatat ttttattatt
    15901 agatttatta tttaatttaa aattagaaat gtaactattt gaaaattctt tttaaaagta
    15961 tatcaaaata tcaaatacat aaaaaaataa tatttgacaa agtatcatat gtgtttacaa
    16021 aatcaacttt a
//
LOCUS       DAGSRM010000089        15979 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.089, whole genome shotgun sequence.
ACCESSION   DAGSRM010000089 DAGSRM010000000
VERSION     DAGSRM010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15979
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.089"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            248..397
                     /gene="rpmG"
                     /locus_tag="MJE33_002923"
     CDS             248..397
                     /gene="rpmG"
                     /locus_tag="MJE33_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBY9648846.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
     gene            423..644
                     /gene="secE"
                     /locus_tag="MJE33_002924"
     CDS             423..644
                     /gene="secE"
                     /locus_tag="MJE33_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBY9648847.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTS
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            664..1206
                     /gene="nusG"
                     /locus_tag="MJE33_002925"
     CDS             664..1206
                     /gene="nusG"
                     /locus_tag="MJE33_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination factor
                     NusG"
                     /protein_id="HBY9648848.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDIDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            1245..1670
                     /gene="rplK"
                     /locus_tag="MJE33_002926"
     CDS             1245..1670
                     /gene="rplK"
                     /locus_tag="MJE33_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBY9648849.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            1741..2439
                     /gene="rplA"
                     /locus_tag="MJE33_002927"
     CDS             1741..2439
                     /gene="rplA"
                     /locus_tag="MJE33_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBY9648850.1"
                     /translation="MAKKGKRYAGALQKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     misc_feature    2491..2619
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            2662..3168
                     /locus_tag="MJE33_002928"
     CDS             2662..3168
                     /locus_tag="MJE33_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBY9648851.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     gene            3226..3591
                     /gene="rplL"
                     /locus_tag="MJE33_002929"
     CDS             3226..3591
                     /gene="rplL"
                     /locus_tag="MJE33_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBY9648852.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
     gene            3983..4771
                     /locus_tag="MJE33_002930"
     CDS             3983..4771
                     /locus_tag="MJE33_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBY9648853.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEGKIDILVNNFGSTNPSLDKDL
                     VTGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPEFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
     gene            5242..8958
                     /gene="rpoB"
                     /locus_tag="MJE33_002931"
     CDS             5242..8958
                     /gene="rpoB"
                     /locus_tag="MJE33_002931"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBY9648854.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIIKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGESFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     AENAEDEDIENLEEFTEDDLFEEEIDFDSDDFDM"
     gene            9000..12485
                     /gene="rpoC"
                     /locus_tag="MJE33_002932"
     CDS             9000..12485
                     /gene="rpoC"
                     /locus_tag="MJE33_002932"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBY9648855.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNENIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKALGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREIDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVIVEADSMIQEDEAETIVALGIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            12774..13196
                     /gene="rpsL"
                     /locus_tag="MJE33_002933"
     CDS             12774..13196
                     /gene="rpsL"
                     /locus_tag="MJE33_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBY9648856.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            13320..13790
                     /gene="rpsG"
                     /locus_tag="MJE33_002934"
     CDS             13320..13790
                     /gene="rpsG"
                     /locus_tag="MJE33_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBY9648857.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            13839..15905
                     /gene="fusA"
                     /locus_tag="MJE33_002935"
     CDS             13839..15905
                     /gene="fusA"
                     /locus_tag="MJE33_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBY9648858.1"
                     /translation="MARKFPLERTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFVNKMDILGADFYNVVSMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
BASE COUNT         6255 a         1987 c         3252 g         4485 t
ORIGIN      
        1 ctttactaaa ctgatggatt aaaaggaata acaaaaagag gggttatgct cctctttttt
       61 taacatagaa atacaaaaac attgacacag cataggattt tgtgataact tatacaaggt
      121 aatgattata ggaagttaat aatatcttga atactgtaga tttttaataa atacatattt
      181 taaagatatt atattcacat atattaacaa tatatattat tgtattacaa agttggaggt
      241 gtatcagatg agagttaaag taactttagc atgtacagag tgtaagcaaa gaaactacaa
      301 cactactaag aataagaaaa acaatccaga cagaatagaa ttacaaaaat attgtagatt
      361 ctgtaaaaag catacaactc ataaagaaac aaaatagttt tttataagga tgtgagtatg
      421 gaatggctgc ccaaaaaaat gacggtgcaa aaactaaaaa aaggtttagt ttgtttggat
      481 acttaaaaga aactaaacaa gaattaaaaa gagttacatg gccgacaaaa aaggaactgt
      541 ttaaaaacac aagtatagtt ttaactgtag ttatttcatg cactatatta gtatggggta
      601 tagacactat attatctggt gcactagcgt tattactaaa atagacgaaa agaggtaagt
      661 taaatgtcag aattacaaga agcaagttgg tatgtagttc atacctattc gggacatgaa
      721 aataaagtta aagctacaat tgagaaagca gttaaaacaa gaggtatgga agactgcata
      781 agacaagtag ttgttccaac agaagaagta gtggaaacta ctaaaactgg aaaagaaaaa
      841 actagacaac gtaaagttta tccaagttat gttttggtta aaatgataat aactgatgaa
      901 tcttggtatg tagtaagaaa tactaaggga gttacaggtt tcgttggacc tggttcaaaa
      961 ccagtgccat taagtgaaga cgaagtcaaa gctatgggga tagacacaac agaccctaaa
     1021 gtagtaaata gcgatattga ttttgaaata ggagatacag taaaagtatc tcaaggacca
     1081 tttagtggac aaataggtaa catagaagaa attgacttag aaaatagaga agtcaaagta
     1141 tgcataaatg catttggtaa aaggactcta tttgtaatag agcttgaagg tatagaaaaa
     1201 atctaatact atattataga aaaatctgag gaggtgcgct caaaatggct aaaaaagtta
     1261 taggtcaaat aaaattacaa atacctgcag gaaaggctac tccagcgcca ccagttggac
     1321 cagcattagg acaacatggt gttaatataa tgggattcac aaaagaattt aatgctaaaa
     1381 ctgcagatca agctggaatg ataataccag ttgttataac tgtatatcaa gatagatcat
     1441 tcagttttat aacaaaaact ccaccagctg cagttttaat taaaaaagca ttaaacttaa
     1501 aatcaggttc tggagaacca aacaagaaaa aagttgctaa aatgacttca gctcaagtaa
     1561 gagaaatagc tgaattaaag atgcctgact taaatgctgc atcagtagaa gctgctatga
     1621 gtatgatagc tggtactgct agaagcatgg gtgttgtaat cgaagactaa ttaaaagata
     1681 cacttttttt tataaagtgg gaggctagaa gccgaaatta accacgagga ggaaaataaa
     1741 atggctaaaa aaggtaaaag atatgcaggt gcattacaaa aagtagatag aactaaattt
     1801 tatgatgcat cagaagcatt aacattagtt tcagatatag ctggagctaa atttgatgag
     1861 acagtagaag cacacattaa attaggtgtt gactcaagac atgctgacca acaagtaaga
     1921 ggtgcagttg tattacctca tggaactggt aaaacaaaaa gagttttagt ttttgctaaa
     1981 ggtgaaaaag ctaaagaagc tgaacaagct ggtgctgact ttgtaggagc tgaagaatta
     2041 gttcaaaaaa tacaaggtga aaactggttt gactttgata tagtagttgc tactccagat
     2101 atgatgggtg tagtaggtag attaggtaga gtattaggac ctaaaggttt aatgccaaac
     2161 cctaaatcag gaacagttac atttgatgta gctaaagcta tagatgaaat aaaagctggt
     2221 aaagttgaat acagattaga caaaactaac ataatacacg ttccagtagg aaaagtatca
     2281 tttggtggag aaaaattaac tgaaaacttt actgcattaa tggatgctat aataaaagct
     2341 aaaccagctg ctgctaaagg acaatattta agaagtataa cagtagcttc ttcaatggga
     2401 cctggagtta aaataaaccc agcaagaaca gctgaataat aaaactgttg acacaggttg
     2461 acaaaaatgt tattatatca cttgtcaatg taaaaaagaa tagatttgcc gtagatagtg
     2521 ggtgcgagag cttaatatcc cgccgaggtt ttagataaat tgagattact aaagcttttg
     2581 atgtctactt ttggcaacaa aagcttttta taatgttgcg gtaaatacta agcctatttg
     2641 gtgaaggagg tgcacatcta tatgagaaaa gctatagaaa ttaaatcaga agttgtttct
     2701 gagatagttg aaaagttaca aaaatcttct gctgctgttg ttgtagatta caaaggatta
     2761 acagttgaag aagtaactga gttaagaaaa caaatgagag aagctggtgt agattacaaa
     2821 gtatacaaaa atactttagt tagaagagca gctaaagaag ttggtataga gcaatttaat
     2881 gatgaattat tagttggaac taatgccata gcatttggat atgatgatcc agttgctcca
     2941 gctagaatat taaaaggatt catggattct catccaaaaa tgaaattaaa gatgggtatt
     3001 gtggaaggtg cattctatga tgaatctaag attgttgaaa tggctaacat accatcaaga
     3061 gaagttctta tcgctaaatt acttggtagc ttaaaagctc cagtatcaaa cttcgcatac
     3121 ttaatagatg caatagcgaa aaaggcagaa ggtcaagaag aagcttaatt gcttaactaa
     3181 aaaattataa atattaattt gaaataaaat tcggaggtgc taaaaatgac aatagaacaa
     3241 atattagaag ctatagaaaa tatgaaagtt ttagaattaa atgaattagt taaagctgct
     3301 gaagaaaaat tcggtgtatc agcttcagct ccagtaatgg tagctggtgc agctgctggt
     3361 ggaccagctg ctgaagaaaa aactgagttt gatgtagtat taactgacgt tggttcttca
     3421 aaagttggag ttataaaagc agttagagaa ataacaggat taggattaaa agaagctaaa
     3481 gaagtagttg acaatgctcc taagacagtt aaagaaggag cttctaaaga agaagctgat
     3541 caaataaaag agaaattaga agctgctgga gctaaagtag aagttaagta gtttctttaa
     3601 aaagataccc attgggtatc tttttttatt tataaaaaac tataaagaaa gtatttggaa
     3661 ttataaagaa agtattttta taaaataaaa aaaatttata taatagtact aaattaaggg
     3721 tatcatatat ggtgtaatgg tattttaatt taacattatt atcaaattaa aatataaaca
     3781 attgttgtaa tgtctatata taaagtatat aattaaacta aaatgtaaaa ttttacattt
     3841 tagtttaaaa aaatacacga gtgttttgta aataaaatgt aataaataat taattaacaa
     3901 atataaattt taaaataagt tagatattta gaaaaatcga aatttgtagg atttaaagga
     3961 catattcaag gaggagttaa taatggaaaa attacaagga aaaattgcag tagttactgc
     4021 agcaacaaaa ggtattggat tagcatcagc agagatatta gcaaaaaatg gagcaactgt
     4081 gtacttagca gctcgttcag aagaattagc taatgaagtt ataaataaaa taagtgcaga
     4141 aggtggttgt gctaagtttg tttactttaa tgctcgtgaa gaagagacct ttacctcaat
     4201 gatagaagag gtagttaaaa aagaaggtaa gatagatata cttgtaaaca attttggttc
     4261 aacaaaccct tctcttgaca aagaccttgt aactggggat acagataatt tctttgacac
     4321 agtaaatact aatttaaaaa gtgtgtattt accatgtaaa gcagcaattc ctcatatgat
     4381 aaagaatgga aaaggaagta tagtaaatat atcaagtata ggttcagtat tacctgattt
     4441 atctagaata gcttactgtg tatcaaaagc agcaatcaac tcattaactc aaaacatagc
     4501 tacacaatat gctaaagaca atgttagatg taatgctgta cttccagggc ttattgcaac
     4561 taaagcagca ttagacaata tgtctccaga gttcataaaa gaatttttaa agcatgttcc
     4621 tttaaatcgt ataggggaac cagatgatat agcaaaagct gttctattct atgctagtga
     4681 tgactcatca tttataacag gggatttact tgaagttgca ggaggatttg gtttacctac
     4741 tccacaattt gcagataata tattaggata attaaattac taatatgcaa tgaaagctta
     4801 tgcattagtt gtagtagaaa tgatattttt ataaagacca tagatataat ttatattgga
     4861 aaggtttgag gttcaaaatt ccaagttaaa tttatttatg gtctttattt tgtatatttt
     4921 aataaattgt atatatttgt ataaatgtaa ttatttacaa tcattttaat atggaattat
     4981 aattaaatag gggggatggt acttgtataa aattttttat agaaaattag acctataaat
     5041 gaaaaatatg gattttatat tggaaaattg tacataatac ataatatatg cttaaaataa
     5101 tttacaaaaa atcaaaaata atgcatagtt tacatataag ataaaaaata tattgaaaaa
     5161 tacttgacat tatgctaaaa ttattagatg cgttagaatg aactattgtt ttatttttaa
     5221 ttattgagag gtgaaaagtg aatgccacat cctgtcacga taggtaaaag aactagaatg
     5281 agcttttcta aaattaagga aatagctgat gtgccaaatc ttatagaaat tcaagtagat
     5341 tcctatgagt ggtttttaaa agaaggttta aaagaagtgt ttgatgatat ttcaccaata
     5401 gaagattata ctggtaatct tatattggaa tttgtcgact attctctaga tgataaacca
     5461 aagtacgata tagaagagtg taaagagaga gatgctacat attgtgcacc tctaaaggtt
     5521 aaggtaagac ttataaataa agaaacaggt gagataaaag aacaagaagt gtttatgggt
     5581 gacttccctt taatgacaga aagaggaact ttcgttataa atggagcaga aagagttata
     5641 gtaagtcaat tagttagatc tcctggtgtt tactatgcgg aagaaagaga taaaacaggt
     5701 aagagattga tttcatctac tgtaattcct aatagaggag catggttaga atatgaaaca
     5761 gattctaatg atgtaatatc tgtaagagtt gatagaacaa gaaaacaacc agttacagtt
     5821 ttacttagag ctttaggaat aggaacagat gcagagataa ttgacctttt aggagaagat
     5881 gagagattat ctgctacatt agaaaaagat aatactaaga cagtagaaga aggtcttgta
     5941 gagatataca aaaaattaag accaggtgaa cctcctactg tagaaagtgc atcatcttta
     6001 ttaaatgctt tattctttga cccaaaaaga tatgacctag caaaagttgg tagatataaa
     6061 ttcaataaga aacttgcttt atgttataga ataatgaata aaatttcagc agaagacata
     6121 ataaatccag aaactggaga agtatttgta aaagctggag aaaaaataag ctatgatttg
     6181 gctaaagcca tacaaaatgc aggtataaat gtagtaaatc tactaatgga tgatgataaa
     6241 aaagttagag ttataggaaa taattttgtg gatattaagt ctcacattga ttttgacata
     6301 gatgatttaa acataaaaga aaaagtacat tatccaactt taaaagaaat tttagatgga
     6361 tatagtgatg aagaagaaat aaaagaggct ataaaatcaa gaattaaaga acttatacca
     6421 aaacatatat tattagatga tataatagct tcaataagtt atgagttcaa tatattctat
     6481 aatataggaa atattgatga tatagatcac ctaggaaaca gaagaataag atctgtaggt
     6541 gaattgttac aaaatcaagt tagaattggt ctttcaagaa tggaaagagt tataaaagaa
     6601 agaatgacgg ttcaagatat ggaagctata acgcctcaag cgttagttaa cataagacca
     6661 gtttcagctg caataaaaga attttttggt agttctcagt tatctcagtt catggatcaa
     6721 acaaatcctc tatctgagtt aacgcataag agaagattat cagcccttgg gcctggaggt
     6781 ctttcaagag aaagagctgg attcgaagtg cgtgacgttc accattcaca ctatggtaga
     6841 atgtgtccaa tagagactcc agagggacca aatatcggtc ttataaactc tctaggaact
     6901 tatgcaaaaa taaatgaatt tggatttata gaatcaccat atagaaaatt tgataaagaa
     6961 acatcaacag ttactgatga aatacattat ttaactgctg acgaagaaga tttattcgta
     7021 agagctcagg caaatgagcc attaacagaa gatggtaaat tcgtaaacca tagagttgtt
     7081 tgtagaactg taaatggtgc tgttgaaatg gttcctgaaa gtagagtaga ttacatggat
     7141 atatctccta agcaggttgt atctgttgcc actgctatga tacctttctt agaaaatgat
     7201 gatgccaacc gtgcccttat gggagcgaac atgcagcgtc aggcagtgcc tctagttaga
     7261 agagaagcac caattatagg aactggtata gaatacagag cagcaaaaga ctctggagca
     7321 gttgttgttg ctagaaattc tggtatagca gaaagagtaa cagcagatga aataattata
     7381 aaaagagaag atggaaacag agatagatat aatctactta agtttaaacg ttcaaactca
     7441 ggtacttgta taaatcaaac acctataata aataagggtg accagataat aaaaggtgat
     7501 gttatagcag atggtccagc aactgattta ggagaagttg cacttggaag aaactgtctt
     7561 atagcattca tgacttggga aggttataac tacgaggatg ctatattaat aaatgaaaga
     7621 ttagttaaag aagatagatt atcaacaatt catatagaag aatatgaatg tgaagctaga
     7681 gatacaaaac tagggccaga ggaaataact agagatatac ctaatgttgg agatagtgca
     7741 attaagaatt tagatgacag aggtataata agaataggtg cagaagtaga ctcaggtgat
     7801 atattagttg gtaaagtaac tccaaaagga gaaactgaac ttactgctga ggaaagatta
     7861 cttcgtgcaa tatttggaga aaaggctaga gaagttagag atacttcact taaagtgcct
     7921 catggtgaat ctggtataat agttgatgta aaagtattta ctagagaaaa tggagatgat
     7981 ttatctccag gagtaaatga attagtaaga tgctatatag ctaaaaagag aaaaataaaa
     8041 gttggagata aaatggctgg tcgtcatggt aataaagggg ttatctcaag agtattacct
     8101 gaagaagata tgccattcat ggaaaatgga acaccactag acataatact taacccacaa
     8161 ggtataccat cacgtatgaa cattggtcag gttttagaag ttcatttagg acttgctgct
     8221 aagacattag gatggtatgt agcaacatca gtattcgatg gtgctaatga gtatgacata
     8281 atggatgctc ttgaagaagc tgggtatcct agagatggta aattaacttt atatgatggt
     8341 agaacaggtg agtcttttga caacagaata actgttggat atatgtacta cttgaaacta
     8401 catcacttag tagatgaaaa actacatgca agaagtactg gaccatactc attagttact
     8461 caacagccac taggtggtaa agcacaattt ggtggacaaa gatttggaga gatggaggtt
     8521 tgggctctgg aagcatatgg agctgctcac atacttcaag aaatacttac tgttaagtct
     8581 gatgacgttg taggtcgtgt tagaacatat gaagctatag ttaaaggtga aaatatacct
     8641 gaaccaggaa tcccagaatc atttaaggtt cttataaaag aacttcaaag tttatgctta
     8701 gatgtaaaag tattgacaga tgaagaccaa gaaatagaag taagagaatc tgtggatgaa
     8761 gatgatacaa taggtgaatt tgagctagat gttgtaaatc atatgggaga agttgaagaa
     8821 agtaatataa tagaagaaat tgaagatgat tttgcagaaa atgcagaaga tgaggatata
     8881 gagaatttag aagaattcac tgaagatgat ttatttgaag aagaaataga ttttgatagt
     8941 gatgactttg atatgtaaat ttaattaatg taaatctcaa atagaaggga gagaactcct
     9001 tgtttgaatt aaacaatttc gagtcgataa aaatagcatt ggcttctcca gaaaaaataa
     9061 gacaatggtc taggggagaa gttaaaaagc cagaaactat aaattaccgt actttaaaac
     9121 cagaaaaaga tggtcttttc tgtgaaagaa tatttggacc acaaaaagac tgggagtgcc
     9181 actgtggtaa atatagaaga gttagatata aaggtgtagt ttgtgataga tgtggagtag
     9241 aagtaactaa atcaaaagta agaagagaga gaatgggaca tatagagcta gcggctccta
     9301 tgtctcacat ctggtacttc aaaggtatac caagtagaat gggactttta cttgatatgt
     9361 caccaagatc gttagaaaaa atattatact ttgcctcata tgtggtagtt gatccaggag
     9421 agactggatt aaatgaaaaa caattgctta cagaaaaaga atacagaact gctcttgaaa
     9481 agtatggata tacttttact gtaggaatgg gtgctgaagc tgtaaagaca ttactacaaa
     9541 atatagattt agagcaacaa agtaaagacc taagagcgga gttaaaagat agtacagggc
     9601 aaaagaaagt tagaacaata agaagattag aagttgtaga ggcatttaaa aagtctggaa
     9661 ataaaccaga atggatgatt ttagatgcaa taccagtaat accaccagat ttaagaccaa
     9721 tggtacaact tgatggtgga agatttgcga cttcagacct aaatgattta tatagaagag
     9781 ttataaatag aaacaataga cttaaaagat tattagagct tggagctcca gatataattg
     9841 taagaaatga aaaaagaatg cttcaagaag ctgttgatgc attgatagat aatggtagaa
     9901 gaggtagacc tgtaacagga cctggaaata gaccacttaa atctttatca gatatgttaa
     9961 aaggtaagca aggtcgtttc cgtcaaaact tacttggtaa gcgtgttgac tactcaggac
    10021 gttctgttat agttgttgga ccagaactta aattttatca atgtggactt ccaaagaaga
    10081 tggcattaga attattcaag ccatttgtta tggataaatt agttaaagaa ggttatgcac
    10141 ataatataaa aagtgcgaaa tctatagtag aaaaagttaa gccagaagtt tgggatgttt
    10201 tagaagatgt tataaaaagt catccagttc ttcttaaccg tgcaccgact ctacatagat
    10261 taggtataca agcatttgaa ccaatcttag ttgaaggtaa ggctataaaa ctacatcctc
    10321 ttgtatgtac agcttataat gcagactttg atggtgacca aatggcagtt catgtacctt
    10381 tatcagtaga agcacaagca gaagcaagat tcttaatgct ttctgtaaat aatatacttg
    10441 ctcctaaaga tggttcacct ataactactc catctcagga tatggtttta ggttgctatt
    10501 atctaacaat agaagcccaa gatggagcta aaggaacagg tatggtattt aaagacttta
    10561 atgaactatt acttgcttat tacaataaat cagttcatct acatgcatta gtgaaactta
    10621 aggtaacact tgaagatgga agaagttcat tggttgaaag tactgttggt agatttatat
    10681 ttaatgaaaa tatacctcaa gacttaggtt ttgtagatag aaaagaaaat ccatttgcac
    10741 ttgaagttga tttcttagca gataaaaaat ctcttggtaa aataatagat aaatgcttta
    10801 gaaaacacgg aaatacagaa acagctgaat tactagatta tataaaagct ctaggattta
    10861 aatattctac attaggtggc ataacagttg ctgttgatga tatgagtgtc ccagaagaaa
    10921 agaaagtatt tatagctgag gcagaagcta aagttgataa gtatgaaaaa gcatacagaa
    10981 gaggtctaat ctctgatgaa gaaagatatg aaaaagttat agagacatgg acagaaacaa
    11041 ctgataaagt tactgatgct cttatgggtg gactagatag attaaataat atatatataa
    11101 tggcacattc aggagccaga ggttctaaaa accaaattag acagctggca ggtatgcgtg
    11161 gtcttatggc aaatgcatct ggtaaaacag ttgagatacc agttaaatct aatttccgtg
    11221 aaggtttatc agtactagaa tactttacat cttcacatgg tgccagaaaa ggtcttgccg
    11281 atacagctat acgtacagct gaatctggat atttaacaag aagacttgtt gatgtaagtc
    11341 aagatgttat tgtaagggaa atagattgtg gaacagaaga tactacagag atttatgcta
    11401 taaaagaagg aaatgaagtt atagaagaga tatatgatag aattgtagga agatatacta
    11461 tagaccctat attaaatcct gaaactggag aagttatagt tgaggctgat tcaatgatac
    11521 aagaagatga agcagaaact atagtagctt taggaattga aaaaattagg ataagaacag
    11581 ttcttaactg taaaactaat catggagttt gttctaagtg ttatggtaga aacttggcaa
    11641 caggaaaaga agttaatata ggtgaagcag ttggtataat agcagctcaa tctatcggtg
    11701 agccgggtac tcagcttaca atgcgtacat tccatactgg aggagttgca ggagctgata
    11761 taactcaagg tctaccaagg gttgaagaat tatttgaagc tagaaaacca aaaggattag
    11821 ctgtaataac tgaagtatct ggtagagttg aaattgatga aacaggaaag agaaaagaag
    11881 taaatgtaat accagaagaa ggcgagactc aaacatatgt aattccatat ggttctagat
    11941 taaaagttaa gcaaggtcaa atgctagagg ctggagaccc tctaacacaa ggatttataa
    12001 atcctcatga tatagtaaga gtaaatggtg ttaagggagt tcaagaatat atagttaaag
    12061 aagttcaaag agtgtataga cttcaagggg ttgatgttaa tgataagcat atagaagtta
    12121 tagtaagaca aatgctatct aaagtaaaag ttgaagaccc aggagataca gatttattgc
    12181 caggtggata tgaagatgta ttaacattca atgaatgtaa taaagatgct atagataaag
    12241 gtctaagacc agcagttgct aaaagagttt tacttggtat aactaaagca tctcttgcaa
    12301 ctgattcatt cttatcagca gcttctttcc aagaaacaac aagagtatta acagaggcag
    12361 caataaaagg taaagaagac cacttaatag gacttaaaga aaatgttata ttaggtaagt
    12421 taataccagc aggaacagga atgaagaaat atagaaatat agctgttgaa aaaattgaag
    12481 attaatagca tatagagtaa agaaatcttg acaaccatta tttgtggtgt taaaatagta
    12541 aagtgtgttg tttaaaagta cactaagggc ttgaacaagt tcttagtgac ttttagatgg
    12601 caataaatga gaactaaaaa gcatcaagct agaaaagtgc taatatcaca tatttatgtg
    12661 ttatagacat taaattgtaa gtaacgaatg ttacaatcag tcagtatgaa tattcatatt
    12721 gatgtagttg gcggtcactg ataagatcga aaatttggaa ggaggtgcag atgatgccaa
    12781 caattaacca attagttcgt aaaagtagaa aagcgctaga gaagaaatct actgctccag
    12841 cattacaaaa aggttacaac tctttaaata agaaagtaac tgacgctagt gccccacaaa
    12901 aaagaggggt ttgtacttca gtaaaaacag ttactcctaa gaagcctaac tcagctttaa
    12961 gaaaagttgc cagagttaga ttaactaatg gtatagaagt ttctgcttat ataccaggag
    13021 aaggacacaa cttacaagag catagtgttg ttcttataag aggaggaaga gtaaaagacc
    13081 ttccaggggt tagataccat atactaagag gtactctaga tacagcaggt gttgataaga
    13141 gaagacaatc aagatctaaa tacggtgcga agagacctaa agaagctaaa aagtaaaaac
    13201 aattaatgat tgattatagt tcggtgcttt aaatatatat atatttacgg catcacggca
    13261 ttaaatgatt tatgtcgagt acctatgatt taagttaaat aattaaggag ggaagcgaaa
    13321 tgccaagaaa aggtaatatt ccaaaaagag aagttttacc agatcctatg tatgggagta
    13381 aggtagttac taaattaata aataatctaa tggtagacgg aaaaaaagga aagtctcaaa
    13441 gaatagttta tgatgctttc gcaattgttg ctgaaaagac aggagaagaa gctttagaag
    13501 tatttaataa agcaatggat aatataatgc ctgttttaga agtaaaagca agaagagttg
    13561 gtggggcaaa ctaccaagtg ccaatcgaag ttagacctga aagaagacaa acattaggtt
    13621 taagatggct agtaaaatat actagagctc gtggagaaaa aggaatggtt gaaaagctag
    13681 ctaaagagat aatggacgca gctaacaaca ctggagcttc agttaagaaa aaagaagata
    13741 ctcataaaat ggcagaagct aataaagcat ttgctcatta cagatggtag gatatataga
    13801 tatcttataa aattgaggat atcataagga ggataaccat ggctagaaag tttcctttgg
    13861 aaagaactag gaatatagga atcatggctc atatagatgc aggaaaaact actactacag
    13921 aaagaatcct gttttatact gggcaaactc ataaaatagg agaaactcat gaaggagctt
    13981 ctcaaatgga ctggatggag caagaaaagg aaagaggtat aacaataact tctgccgcta
    14041 ctactgcttc atggaaagac catagaataa acataataga tactcctgga cacgtggact
    14101 tcacagttga ggttgaaaga tctctaagag ttcttgacgg ttcagttgct gtattctgtg
    14161 caaaaggtgg ggttgagcct caatcagaaa atgtatggag acaagctgaa acttatggag
    14221 tacctagaat agcttttgta aataaaatgg atatattagg tgcagatttc tacaatgttg
    14281 taagcatgat gaaatctaga ttaaattcta atgcagttcc tatgcaatta ccaataggta
    14341 aagaagattc atttataggt ataatagact tattaaaaat ggatgctgtt atatacaaag
    14401 acgacctagg ggttgaaatg gaagaaacag atataccaga agatatgaaa gaattagctg
    14461 ctgagtggag agaaaaatta gttgaatcag tagctgaaac tgatgaagaa ttaatgatga
    14521 aataccttga aggtgaagaa cttactatag atgaattaaa agttgctata agaaaagcta
    14581 ctatagcttg tgaaatgaac ccagttttct gtggtacagc ttatagaaac aaaggtgttc
    14641 agttagtaat agatgcagtt ttagattact taccagcgcc aactgatata ccagctataa
    14701 aaggtatatt agcagatgga gaagaagctg aaagacattc ttctgatgaa gagccattct
    14761 ctgcattagc attcaaaata atgactgacc cattcgttgg aaaactagcg ttcttcagag
    14821 tttattctgg aacattagaa tcaggttctt atgttttaaa tgcaactaag aataagagag
    14881 aaagaattgg tcgtatatta caaatgcatg ctaatacaag agaagaaata actaaagtgt
    14941 atgctggaga tatagcagca gcagtaggat taaaagatac tactactgga gacacattat
    15001 gtgaccctgc taatccaata atacttgaat ctatggaatt ccctgagcca gttatatctg
    15061 ttgctataga accaagttct aaagcagctc aagaaaaaat gggtatagct cttcaaaaat
    15121 tagctgaaga agacccaact ttcacagtta agactgacca agaaacagga caaactataa
    15181 tatctggtat gggtgaatta cacttagaga taatagtaga tagattatta agagaattta
    15241 aagtagaagc taaagttggt gcaccacaag ttgcatatag agagactata actcaaccag
    15301 ttgatgttga atacaagtac tctaagcaat caggtggtag aggacaatac ggtcacgtta
    15361 agattagagt tgctcctcaa gagccaggtg aaggatacaa attcacaaac aaaactgttg
    15421 gtggatcagt accaaaagaa tatgttggtc cagttgacat gggtatacaa ggagctatgc
    15481 aatctggtat agttgctgga tatccagttg tagacgttgc tgttgagtta tatgatggtt
    15541 cttaccatga ggttgactct tctgagatgg cgtttaagat ggctggttca atggcattca
    15601 aagatgctat gaagaaaggt aatgcagttc ttttagagcc ttacttcaaa gtagaagttg
    15661 ttactccaga agattacatg ggagatgtta tgggtgactt aaactcaaga agaggattaa
    15721 tccaaggtat ggaagctaga agtggtgcac aagttataaa tgcattcgtt ccactttcag
    15781 aaatgtttgg atactcaaca gacctaagat cttcaactca aggtcgtgca acttacacaa
    15841 tgatattcga tcactatgag caagttccag cttcagttgc taagaaaata gctgaaggaa
    15901 aataatttaa aaataattaa taatataaat ataaaaaata atcagctaga tgagagatat
    15961 ctctcatcta gcacaagat
//
LOCUS       DAGSRM010000090        15784 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.090, whole genome shotgun sequence.
ACCESSION   DAGSRM010000090 DAGSRM010000000
VERSION     DAGSRM010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15784
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.090"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            404..709
                     /locus_tag="MJE33_002936"
     CDS             404..709
                     /locus_tag="MJE33_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648859.1"
                     /translation="MPSMIMEVVGVLFIAIGLILTFIEIKGNFPSFYGFGQNSIGKNA
                     VGIIAAGIALIFFRNGIDSIDSIATYGVFLAIELVVIVAYFLIRKKFTKEDKHLRTN"
     gene            1117..1806
                     /locus_tag="MJE33_002937"
     CDS             1117..1806
                     /locus_tag="MJE33_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY9648860.1"
                     /translation="MDILLVEDDKNIREMVYDFLISENYSVKTAIDGNTAIEIFKNYS
                     FDIILLDLMLPKSSGFEVLKYIRSVSVVPVIITTAKDSDTDKMLGLNLGADDYITKPF
                     SIVELLARIKANIRRATVYSSSRNSQDMVIQYKDLIINISKYVVSKNGIDLNLTHTEF
                     EIVKLLATNQGCAFSKEQLYNAIWKEPYYGNENVLNTHINRLRNKLSDGIEKNNYIKT
                     LWGIGYKMEEE"
     gene            1806..2723
                     /locus_tag="MJE33_002938"
     CDS             1806..2723
                     /locus_tag="MJE33_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY9648861.1"
                     /translation="MELFLVVISIVLFALYLKERNKSKKMESDYKCINSRLRDITNDD
                     EINYILVPSDISIVKETAVDINKLLEKFYSRQINYNRSEKAILQIFTNISHDLRTPIT
                     VLKGYIEMLYLQSQKEELSSSMRATVEKMQTNSNELVNSINNLFNMAKIQSGDMILNL
                     QKSNLTQLCHEIILEFYDLLDKENFNVEVNIEDRPIYANVDTEALQRILKNLIDNAIK
                     YGSDGRFLGISLYEKEKHVFIDIEDKGSGISETEKEHIFTRAYTADRKKGNGLGLAIS
                     QGLANSMGGSIYVNSRPNVKTVFTVKLKS"
     gene            2796..3722
                     /locus_tag="MJE33_002939"
     CDS             2796..3722
                     /locus_tag="MJE33_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9648862.1"
                     /translation="MNYIIETHQLKKIYKDKAVVNAVNIHVKKGEIYGFVGPNGAGKS
                     TVMKMLLNLVKPNSGEIVMFSKKVEETDFEILKKIGTIIENPYLYENLTAKQNLDLHC
                     EYMGYYNKEHISEVLECVGLSKQSNKKVSKYSLGMKQRLAIARAILTKPELLILDEPI
                     NALDPEGIREMRELFRKLNTDYGITIFISSHILSEVEQIADTIGIIQDGKLIKEISMS
                     DIHKYQTDYVEVDVDNVELAGYLLEKEFGIKDFKITSESCIEIYDLRKDVKEISKIFI
                     QNQIGINSIGRKQSSLEDYFFQTTGTGGKETL"
     gene            3719..4399
                     /locus_tag="MJE33_002940"
     CDS             3719..4399
                     /locus_tag="MJE33_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9648863.1"
                     /translation="MIHLMKLELKKVGLKRYFLFSMLGIFLCMFFVFTGLNDKSTSIY
                     DYDVTFRTISLFFCFYYIILFAVLNVAYIINEYTNKTILILFSYPLDRKKLILSKLLL
                     ITTLMISSMIVGYICCFTFVVVLDKYFNLVIGDFHISILSYWIPGAIKSIIIFCSLGI
                     WTFVVGMIRKSIPVTLVSSIIFINLRQFILAGTNTTQDSLISVFIIVGITVIGIYYIL
                     NHKVTEID"
     gene            4497..4832
                     /locus_tag="MJE33_002941"
                     /pseudo
     CDS             4497..4832
                     /locus_tag="MJE33_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427885.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
     gene            5816..6466
                     /locus_tag="MJE33_002942"
     CDS             5816..6466
                     /locus_tag="MJE33_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY9648864.1"
                     /translation="MKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEVSRT
                     PVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEEEEV
                     FQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQGRF
                     IVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCEDE"
     gene            6815..8881
                     /locus_tag="MJE33_002943"
     CDS             6815..8881
                     /locus_tag="MJE33_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBY9648865.1"
                     /translation="MKEVVGSLKKITKERTENTMKKLFTSETFLEYQFISDCQISPDG
                     GYTAFIVKKADIKENGYTSQVYILNNKTGELKQITSINSVGAYAWEDENTILFPALRN
                     EKVKEAVKNGKQCMSYYALSLNGGEAEELFRLPIKGGKLNPIGKGLYAVIDSYDNDRP
                     IVEGLPEEEQQKKINAYNKRHYEHFKEIPYAVNGEGYISRKRKRLYIYDSNINELKAI
                     TAPMFNVVGMKISDGKILYIGQEFKDVKGLKNGVYVFDTNTNTNVCILEKDKYIIKGF
                     ELYQNQVILNLTDALSYGNGENGDFYTIDIDTKEMKLLSGHQHHCIGNTVTSDVKMGA
                     GQTTKVDGEYIYYTSTVDMDCIIERIHIPTGKQEKVTQTGSVDFIDVKDGNIVCVGCL
                     GNGLPEVYTVENGTLCKKTHLNDHILEEYKISVPEYIESKGSSKWEIQGYVYKPVDYE
                     VGKKYPAVLAIHGGPRLTYGPYFMHEIQVFTSAGYFVFFCNPRGSEGRGNAFADIRKQ
                     FGDIDYIDFMEFTDTVLEKYTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRS
                     IANWSGMEGTTDIGYYFCKGQTGASHMENHELQWKQSPLAYADKCVTPTLFLHGEKDY
                     RCYMQEAFQMFSALKIHGCPTKLCLFEGENHELSRSGRPKQKLQRLVEMLDWFSVYIK
                     KDNIGQ"
     gene            8898..10853
                     /locus_tag="MJE33_002944"
     CDS             8898..10853
                     /locus_tag="MJE33_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBY9648866.1"
                     /translation="MEKIKIESSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWISNTELLFTSKRNKPKSGTTDFYTISIEGGE
                     AKKAFSIPKACSVPVSLGNKLWLLTTKNPTDSKKSEPDRAVEGVDYWTFTDKPFIRDG
                     ENFSQRRRVTLELYQEGENITKAITPKFCEVAGVDVSSDKNRILYTGQIYEDCATPFS
                     GLWEYHIDSGETKELVPQGKYQISLAKYIGKDKVMLQASTLDRSITQNHDIFILDLST
                     NEINMIASPDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFNEFDTKTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTAFSKPYLDTHKV
                     SLPEKLTFVAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDTCIEKYPGIDADRLGVA
                     GGSYGGYMTNWIIGHTDRFKAAASQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQLGVDTEMYLFHGDT
                     HGLSRNGRPSNRIARANAIVDWFERYL"
     gene            10869..12059
                     /locus_tag="MJE33_002945"
     CDS             10869..12059
                     /locus_tag="MJE33_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBY9648867.1"
                     /translation="MSDMLLWFSVVLVLFAIGDLIASKTKAKVSAVFVTLLLFLILFV
                     TKAIPADIIEKAGMTAAASWSVPMIMFSMGTMLNVKQFIDEWRTVLTAWLGIVAVIVC
                     VSLCIPLFGKSTVLTSIPVINGALPATTIMTQAALEKGLTLAAATATVVFAIQKFIGT
                     PIASRAALQEANRLLVEYHEAKSKGIDLANVDTDKKEAENTGTKVKQAFCEKYDKYYS
                     TNVCIFFIALFSYLGYELSEIIHVNYSIVCLVVGVIVTRIGIVPKDILEKGKIKGFIN
                     MVVFAAVIPSLAKVSLTDLISLFVPIVGMFAASIIGIFLMMKVLPGWKIIGSKPLAFG
                     VGFCQMLGFPTTYLISNEVCNAVGETEEERAYLMSKIMPKLVVGGMACMISIVVAGLM
                     VPML"
     gene            13208..13582
                     /locus_tag="MJE33_002946"
     CDS             13208..13582
                     /locus_tag="MJE33_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY9648868.1"
                     /translation="MKVLISNTSDKPLYQQIKEQIKEAIFTEELKEGDAMPSIRNFAN
                     DLKVSILTIRRVYEELENEGFLTRQVGIGTFISTGNLEILRDSKRRIVEQKMADMIKD
                     AKALGITQIELQEMMDILYQED"
     gene            13584..14441
                     /locus_tag="MJE33_002947"
     CDS             13584..14441
                     /locus_tag="MJE33_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9648869.1"
                     /translation="MENILEVYNLNKTYTDFSLSNISFALPEDCITGFIGVNGAGKTT
                     TIRSILDVVKRDSGKIKIFGKDFDGNEKELKNRIGVVFDDSYFYEELSMAEMKSIIAP
                     SYDNWCEEDYQQNMKRFNLNPKQKIDTLSKGMKMKFALALALSHKAEFLIMDEPTSGL
                     DPLIRSQILDILTEYMKKCGRGVFFSTHITSDLDKIADMIVMIDNGRIILEREKDTLL
                     DEHRKVKGDIQFLNDETKKLFLNLEITGYGFIGITSKINEVQKNMPDILIERPTIEDI
                     MLAYIERRE"
     gene            14444..15082
                     /locus_tag="MJE33_002948"
     CDS             14444..15082
                     /locus_tag="MJE33_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBY9648870.1"
                     /translation="MLIHLVKKDILIVKKYVLLILFIAIGIPLFILWRLPPFAQILGF
                     VLSIIFSEFMLCQYLLLKESQYSKASVLLCSTPYPRRGLVQSKYVLFAMVFIYCTLVY
                     WIENLLIPQIGTFDLTYILIVLLISSIIYGVYMPIQYKLGYETTKFFFVVIIMASSFV
                     LPLTIAHSNFEIPFIYQYPSMIVNIVLFLLSLIILLASMFISIKIFSNKELV"
BASE COUNT         5929 a         1763 c         2685 g         5407 t
ORIGIN      
        1 aaaataaatg atttatagat gaagaactaa actcatagat taaaaaataa atgacttata
       61 gatgaaaaat caagctcata gattaaagaa taaataactt attagatgaa aaaacaagct
      121 tatagataag aagtccatgc ttataagcca gaaaatgaat actttaatag attaaaaaat
      181 acatatttgt gaattataga atgaatgact ccataaacta gaaaatttat aataatatct
      241 atatgaaaat tagaattatt ttatcacaaa aaaattaagt taaataaata tgaaattact
      301 tccaaatata cttccaaaaa catgtaatcg catatataat ataattataa aaataataaa
      361 tatttttata gtagtagtta attattaata aggaggggtc aaaatgccta gcatgattat
      421 ggaagttgta ggagtactat ttatagctat tggattaatc ttgacattca ttgaaataaa
      481 aggaaatttc ccttcttttt atggttttgg tcaaaattct atagggaaaa atgctgtagg
      541 tattatagca gcaggaattg ctttaatttt ctttagaaat ggcatagata gcatagattc
      601 tatagctact tatggtgtat ttttagcaat agaacttgtt gtaatagttg catatttctt
      661 aataagaaaa aaatttacta aagaagataa acatttaaga actaattaac taaattatct
      721 actataaaat tggaaaatta aaaacaaata aaagtttgtt tttaattatt taaaatccaa
      781 atttgattat ttttaaagaa actatgttct gtataaaact agaatgtggt ttctttttta
      841 tgtataaaat attttttagc aaaagttaaa aggtgtttta taatataaaa tagttaattg
      901 tagatgttaa taaaagaaaa ttatgtatat ttcagataaa gataagagag tatactatat
      961 tttaattctt ttttcaataa agcttattga aaagaacttt aacttagaag agtatcacca
     1021 aattgcagat acaatatcat aagcagttat ttattgacaa ccctttttaa taaatatatc
     1081 atttgaagta tactgacgaa aaggagttga ataataatgg acatcttatt ggttgaagat
     1141 gataagaata ttcgagaaat ggtatacgat tttctgatta gtgagaatta ctctgttaaa
     1201 actgccattg atggaaatac ggctattgaa atatttaaaa attattcatt tgatattatt
     1261 ttactggact tgatgttacc aaagtctagt ggttttgaag tattaaaata tatacgaagt
     1321 gtgagtgttg ttcctgttat tattactact gcgaaagata gtgacacaga taaaatgcta
     1381 gggcttaatc ttggtgcaga tgattatatt acaaagcctt tttcaatcgt agaattgctt
     1441 gcaagaatta aagctaatat tagacgtgca acagtgtaca gcagttcaag aaactctcaa
     1501 gatatggtta ttcagtataa agatttgatt ataaacatat caaaatatgt agtttcaaag
     1561 aatggaattg acttaaactt aacacataca gaatttgaaa ttgttaaatt actagcgact
     1621 aatcaaggat gtgctttttc aaaggaacaa ttatacaacg ctatttggaa agaaccttat
     1681 tatggaaatg aaaatgtgct aaatactcat attaacaggt taagaaataa attgtctgat
     1741 ggtatagaaa aaaataatta tattaaaact ctttggggaa ttggctacaa aatggaggaa
     1801 gaataatgga gttattttta gttgtaattt cgattgtact atttgctctc tacttaaaag
     1861 aacgaaataa atcaaagaaa atggaaagtg attataagtg tatcaattca agacttcgtg
     1921 acataacaaa tgatgatgaa attaattata ttttagtacc atctgatatt tcgattgtaa
     1981 aagaaacagc agtagatata aataaacttc ttgaaaagtt ttacagcagg caaattaatt
     2041 ataatagaag tgaaaaagcg atattgcaaa tatttacaaa catatcacac gatttacgaa
     2101 ctcccatcac agtattaaag ggttatattg aaatgctata tcttcaaagt caaaaagaag
     2161 aattatcttc ttcaatgaga gcaactgtgg aaaaaatgca aacaaattca aatgagcttg
     2221 ttaattcaat aaataacctg tttaatatgg caaaaatcca atctggagat atgatattaa
     2281 atcttcaaaa atccaatcta acacaattat gtcatgagat tattttagag ttttatgatt
     2341 tgctagataa agaaaacttt aatgttgaag ttaatattga agatagacct atatatgcaa
     2401 atgttgacac agaagcatta caacgtatac tcaaaaattt aattgataat gctataaaat
     2461 atggttcaga tggtagattt ttagggattt cactatatga aaaggaaaaa catgttttta
     2521 tagatattga ggataaaggt tcaggtattt ctgaaactga aaaagagcat attttcacaa
     2581 gagcttatac tgctgacaga aaaaaaggca atggtttagg tttggctatt tctcaagggc
     2641 ttgcaaactc tatgggaggt tcaatttatg taaatagtag acctaatgta aaaacagttt
     2701 ttacggtaaa attgaaaagt tagaaaaaag ttagatttaa aagagagaaa agacaatttt
     2761 attttgtata ctgtttttag aaaggaggga gtatgatgaa ctatatcata gagacccatc
     2821 aattaaagaa aatatataaa gataaagctg ttgttaatgc tgttaatatt catgtgaaaa
     2881 agggagaaat ttatggattt gttggaccaa acggggcagg taaaagtaca gtaatgaaaa
     2941 tgttgctaaa tcttgttaag ccaaattctg gagaaatagt tatgttcagt aagaaagtgg
     3001 aagaaactga ttttgaaata ttaaaaaaga ttggtactat tattgaaaat ccgtatttat
     3061 atgagaattt gactgctaaa cagaatcttg atttacactg tgaatatatg ggttattata
     3121 acaaagaaca tatatcagag gtattggaat gtgtcggatt gtcaaagcaa tcaaacaaga
     3181 aagtttcaaa atattcttta gggatgaaac aacgtcttgc aatagctaga gcaattctta
     3241 ctaaaccaga attattgatt cttgatgaac ctataaatgc actcgaccca gaggggataa
     3301 gagaaatgcg agaattattc cgtaagttaa atacagatta tggaataact atatttattt
     3361 ctagtcatat tctttcagag gtagaacaaa ttgcggacac tattggaatt attcaagatg
     3421 gtaaacttat taaagaaatt tctatgtcag atatacacaa atatcaaaca gattatgttg
     3481 aagtagatgt agataatgtg gagcttgctg gatacttgtt agaaaaagaa tttggaataa
     3541 aagactttaa aattacttcg gaaagttgta ttgaaatata tgatttacga aaagatgtaa
     3601 aagaaatatc caaaatattt attcaaaatc aaattggtat aaatagcatt ggacgtaaac
     3661 aaagttcttt agaagattac ttcttccaaa ctacaggtac aggaggtaaa gaaacactat
     3721 gatacattta atgaaactag agctaaaaaa agttgggcta aaaagatatt ttttattttc
     3781 aatgctgggg attttcttat gtatgttttt tgtctttact ggattgaatg ataaatctac
     3841 ttcaatatat gattatgatg ttacatttag gacaatcagt ttatttttct gtttttacta
     3901 tattatatta tttgctgtgt tgaatgtggc atatataata aatgaatata caaataaaac
     3961 aatcttgatt ttattctctt atccacttga ccgtaaaaaa cttattttat caaagttact
     4021 tttgattacg actcttatga tatcatcaat gatagttgga tatatctgtt gttttacatt
     4081 cgttgttgtg ttggataagt attttaattt agttattgga gattttcata tttcaatttt
     4141 atcttattgg ataccaggag caattaaaag tattattata ttttgctcat taggtatatg
     4201 gacattcgtt gttggaatga taagaaaatc aatacctgtt acccttgtca gttcaattat
     4261 ttttatcaat ttaagacaat ttattttagc tggtacaaat acaactcaag acagtttgat
     4321 ttctgttttt attatagttg gtataactgt gattggtata tattatatac taaatcataa
     4381 agttactgaa attgactaga tactgtggct aggaaggtag ttattaattg tatcttcttg
     4441 ggataataat gatttgctat tatatcagct attattaata ttattagagt tttaataata
     4501 ttgcacaacc atttgaagtt gagccaaagc ttgatgaatt aattgaatgg tactatagtc
     4561 agagtgaagt atctattaaa gttatagctg aatttcatta tagatttgaa ttgatacatc
     4621 catttcaaga tggtaatggt cacataggta gatttgttat gctaaaacag atgttagaaa
     4681 ataatcttcc tataaaaatt gtatcttggg atagtgagga cttgtataga aactccttaa
     4741 attcttgtag tttaggtaat tatgtccctt taattgaata tttatcaagt ttagaagatt
     4801 ttagagaagt atataaaatg ttatggaaat aattgtttgc agcaaaaaaa aattttatat
     4861 aaatatattt ttatctatgc tatttaacgc taatttctta gttattctta gcttggacta
     4921 tatccactat tttaactata taaaatagtg gatatagttt aactatctct ttataactat
     4981 tcttatgtat cttagttgtt tcttaatatg gggacagttc ataaaactaa aagtgatttt
     5041 taattttatc atttataatt taccattaca tcagaaaaat gcttatcatt agaattttca
     5101 taaaagtcac ctattttttt caattaacta aatcaattct attagtctta atctttcgta
     5161 tttcaaattg ataatataag aaaataaaaa ataaaaaatt ttaaattttt tatttattgc
     5221 gaaaaaaata aaaaggtgct atactttttg taacatatta ttcaaattat taagagatag
     5281 taaacattta tagtttaatt tgtagtacac tagcatgatt tgcagaacaa tgaattaatc
     5341 tatagtataa tagtttaatt taaagtacaa cgatttaatc tatagtacac tagcatgatt
     5401 tgaagtacaa cgatttaatc tatagtacaa taacttgatt tgcaatacaa tgaattaatc
     5461 tatggtataa tagtttaatt tgaagtacaa tgaattaatc tatagtataa tagtttaatt
     5521 tgaagtacaa cgatttaatc tatagtataa tagtttaatt tgaagtacaa tggtttaatc
     5581 tatagtataa tagtttaatt tgaagtacaa cgatttaatc tatagtacaa taacttgatt
     5641 tgcaatacaa tgaattaatc tatagtataa gagcatgatt tacagtacaa taatttaaat
     5701 tgtagtacaa aagtactatc aaataagaat ttctacttta aaaatattac taaaagtaat
     5761 taaagtccag taattcaata taatatggca aaagaataga aataaggtga gagtagtgaa
     5821 agtaaagtca aatttagatg aaattttata tcataagatt attgaaagtc ttattcgtgg
     5881 tgaatactct gtaggtcaaa aaatattact aaatgacctt tgtgaaaaat ttgaagtaag
     5941 cagaacacca gttgtacaag cagtaaaaat gctaaataaa gatggagtgc ttactattat
     6001 gacaaatggt aaagtgtacg taccagagta tgaatatgat atggtaaaac aagtttgtga
     6061 aacgagaaca ctgattgaaa catatgcttt agaaaaaatg atgcaagaag aggaagaggt
     6121 ttttcaacaa aagctagatg taataaaaag atactctgat aaatgcgagg agtactatcg
     6181 tcaagaaaaa tctgtagaac ttgcactagc agatttggaa ttacataaag ccattgtaga
     6241 aggggcaaat aatgagattt taaatgatgt atatgttggt atacaaggaa gatttatagt
     6301 agtgaactat ttaattcgac cattaaaaaa tcgtaattat gaaggaacag tacaagacca
     6361 ttttgaaata ttaaaattca ttgaagacag agatacaaaa aaagcagtag ataaactaag
     6421 aaatcatatt caaggaacta ttaaaagatt ctgtgaagat gaataataag ttttgaagtt
     6481 actatatata cttatctatt aagttgctaa atgtagtcta agtataatct aattatagtt
     6541 taaacataat ctaaatataa tctaataaat ataatctaaa tatagtgtaa ataagtctaa
     6601 gtatgaatta aatatagatt aagtatggac taagtataga ttaagtatag attaagtata
     6661 gattaagtat agattaagta tgaattaagc atggattaaa tatactttaa attacaaata
     6721 ttacaaggaa gccagaaggc ttcctaaaaa aataaccttt ataaaatttt ttattttaaa
     6781 tttaaaattt tttattttaa atttaaaata aaaaatgaaa gaggtggtag ggagtttgaa
     6841 aaaaattact aaagaaagga cagaaaatac tatgaaaaag ctatttacga gtgaaacatt
     6901 tttggaatat caatttataa gtgattgcca aatatcacca gatggaggat atacagcatt
     6961 tattgtaaaa aaagctgata tcaaagagaa tggttacaca agccaagttt atatattaaa
     7021 caataaaact ggcgaattaa aacaaataac gtctataaac tctgttggag catatgcctg
     7081 ggaagatgaa aatactatct tgttccctgc tttgagaaat gaaaaagtaa aagaagctgt
     7141 caaaaacggt aagcaatgca tgagttacta tgctttatct ttaaatggtg gagaagcaga
     7201 agaacttttt agactcccta tcaaaggtgg taaattgaat cctataggaa aaggtttgta
     7261 tgcagttatt gattcttatg acaatgaccg acctattgta gagggattgc cagaagaaga
     7321 acaacagaaa aaaataaatg cttataacaa aagacattat gaacatttca aagaaattcc
     7381 ttatgctgtc aatggggagg gatatattag cagaaaaaga aaacgcctat atatatacga
     7441 cagtaatata aatgagttga aggcaattac agcacctatg tttaatgttg taggtatgaa
     7501 aataagtgat ggaaaaatct tatatatagg gcaagagttt aaagatgtaa aaggtttgaa
     7561 aaatggggtc tatgtatttg atacaaacac aaacacaaat gtctgtatat tagaaaaaga
     7621 taagtatatt ataaaaggtt ttgaattata tcaaaatcaa gtgatactaa acctaactga
     7681 tgctttatct tatggaaatg gagaaaatgg agatttttat actattgata ttgatacgaa
     7741 agaaatgaaa cttctttcag ggcatcaaca tcattgtatt ggaaatacag taacatctga
     7801 tgttaaaatg ggtgctggtc agacaacaaa ggtagatgga gaatatattt attacacttc
     7861 tacagtagat atggactgca ttattgaaag aatacatatt ccaacaggaa aacaggaaaa
     7921 agtaacacag acaggttcag ttgattttat agatgtaaaa gatggcaata ttgtatgtgt
     7981 gggttgttta ggaaatggat taccagaagt ttatacagta gagaatggaa cattgtgtaa
     8041 gaaaacacat ttaaatgacc atattttaga ggaatataaa atttctgtgc cagaatacat
     8101 agagtcaaaa ggaagttcta aatgggaaat acaaggatac gtatataaac cagtagatta
     8161 tgaggtgggg aaaaaatatc cagctgtatt ggcgattcat ggaggaccaa gattgacata
     8221 tggaccatat tttatgcatg agatacaggt atttacaagt gcaggttact ttgtattctt
     8281 ctgtaaccca agggggtctg aaggtcgtgg caatgctttt gcagatatta ggaaacagtt
     8341 tggagatatt gattatatag attttatgga atttacagat actgtacttg aaaaatatac
     8401 agacattgat aaaacaaaat tggctgttga aggtggaagt tatggtggat ttatgacgaa
     8461 ttggataatt ggtcatacaa accgttttgc tgttgcttgt gctcagcgtt ctattgcaaa
     8521 ctggagtggc atggagggta caacagacat tggttattat ttctgcaaag gacaaacagg
     8581 tgcttctcat atggaaaacc acgagcttca atggaaacag tcacctcttg cttatgctga
     8641 taaatgtgtt acaccgactt tatttttaca tggtgaaaaa gactatagat gctatatgca
     8701 agaagcattc cagatgtttt cagcattgaa aattcatggg tgtcctacaa aactttgtct
     8761 atttgaagga gaaaaccatg aactgtctcg ttctggtaga ccaaaacaaa agctacaaag
     8821 attagttgaa atgctagatt ggttttcggt atatataaaa aaagataaca taggacagta
     8881 gaaaggagaa tataaaaatg gaaaaaataa agattgaaag ttcagatatt tttgaatata
     8941 tattccctca tgatatttct tgttcacctg atggaaagca tattgcttat attatatcaa
     9001 atatcaacga ggaaaaagac tgctatgaac atgacttata tgtgatggac atcaaaacag
     9061 aaaaacaaat acatatgact caaacaaaag atgtaacatc atttagttgg atttctaata
     9121 cagaactcct ttttacatct aaaagaaata agccaaaatc aggtacaaca gatttttaca
     9181 ctatttctat agaaggagga gaagcaaaaa aagcattttc tattccaaaa gcttgtagcg
     9241 tacctgtatc acttggaaat aaattgtggt tactgacaac aaaaaatcca acggattcta
     9301 aaaaatcaga accagatagg gcagtagagg gtgtagatta ttggacattt acagataaac
     9361 cttttataag agatggagaa aatttttctc aaagaagacg tgtaacatta gaattatatc
     9421 aagaaggtga aaatataaca aaagccatta caccaaaatt ttgtgaagta gcaggtgtag
     9481 acgtttcttc tgataaaaac agaattttat atactggtca gatttatgaa gattgtgcaa
     9541 ctcctttttc tggactttgg gagtatcata tagacagtgg tgaaaccaaa gaacttgtac
     9601 cacaaggaaa gtaccaaata agtttggcaa aatatattgg caaagataaa gtaatgttgc
     9661 aagcttcaac attagaccgt tctatcacac agaatcatga tatttttata cttgatttga
     9721 gtactaatga aataaatatg attgctagtc cagatggtat gtatgctaca ttgctggatg
     9781 tagatgcagt atatggtggt ggacgtagta ataaagttat aggagataaa tttattggtg
     9841 caagaatttg cagaactatg acagaattta atgagtttga tacaaagaca ggtaatatac
     9901 gtatcatcac aaaagtagat gcttttacat cttttgatat ttacgacaac accatgtaca
     9961 cagtaatgtt gaaagattat gagttagcag aaatatatag cattgatatg actacaggta
    10021 caatgaaaaa aatgactgct ttttctaaac catatttaga tactcataag gtttcattac
    10081 ctgaaaaatt gacgtttgta gcaaaaaata aagaagaagt tgatggtttt gttataccac
    10141 ctatagatgc taaagaaggc gaaaaatatc ctgctgtatt gttcattcat ggtgggccta
    10201 aatgggcata tggctatatg tttacacact tgaagcagtg tgtaacatca aagggaatgt
    10261 atgtgattta ttgtaaccct catggtggtg acggttatgg tgaaaagttc ttagaaatgg
    10321 tagaaagatg ggggtatgtt gactatgagc atcttatgga atttgtagat acttgtattg
    10381 aaaaatatcc tgggattgat gcagaccgct taggggttgc agggggaagt tacggtggtt
    10441 atatgacaaa ctggataatt ggtcatacag accgtttcaa agcagcagct tctcaaagag
    10501 gtattagtaa cttgattaca gcttctttaa ttattgactt tggagataga attatgaagc
    10561 agacttgtgg agataagacg ccttggaatc atgaggaggt tctttggaat cattcaccta
    10621 ttaaatatgt gaaaaatgtt aagacaccaa cattgttctt acatagcgat agggattata
    10681 gatgttttat gggggatact ttccaaatgt ttacagcatt aaaacagctt ggtgtagata
    10741 cagaaatgta tttgtttcac ggagatacac atggattgtc tagaaatgga agaccatcta
    10801 atagaattgc aagagcaaat gctattgtag attggtttga aagatattta taataagggg
    10861 gttattctat gagtgatatg ttattatggt ttagtgtcgt attagtttta tttgctattg
    10921 gggatttaat tgcaagcaaa acaaaagcaa aagtatcagc tgtatttgtt acactacttt
    10981 tatttttgat tctatttgtc acaaaagcaa taccagctga tattattgaa aaagcaggta
    11041 tgaccgcagc agctagttgg agtgttccaa tgattatgtt tagtatggga acaatgttga
    11101 atgttaaaca atttatagac gaatggagaa cagtattgac agcatggctt ggaattgtag
    11161 cagttatagt ctgtgtctca ttatgtattc ctttgtttgg aaaatcaaca gtattgacaa
    11221 gcataccagt tataaatgga gctttacctg ctactactat tatgacacaa gcagcactag
    11281 aaaaaggact gacacttgca gcagctacag caacagttgt atttgcaatt cagaagttta
    11341 ttggtacacc aatagcttca agagcagcgt tacaagaggc aaaccgtctt ttagtagaat
    11401 atcatgaagc aaaatcaaaa ggcattgatt tagcaaatgt tgatactgat aagaaagaag
    11461 cagagaatac aggcacaaaa gtaaaacaag ctttctgtga aaaatacgat aaatattatt
    11521 ctacaaatgt ttgtatattt tttatagcac tattctctta tctagggtat gaactttcag
    11581 agattattca tgtaaattat tctattgtat gtcttgttgt tggtgttatt gtaacacgta
    11641 ttggtattgt accaaaagac attttggaaa aaggaaaaat caagggattt atcaacatgg
    11701 ttgtgtttgc agctgttatt ccttctttag caaaagtttc tttgacagat ttaattagtc
    11761 tttttgtacc aattgtgggt atgtttgcag cttctatcat tggtatattc cttatgatga
    11821 aggtgttacc aggttggaaa attatcggtt ctaaacctct tgcttttgga gtagggtttt
    11881 gtcagatgtt gggattccca actacatatt taatttcaaa tgaagtgtgt aatgctgttg
    11941 gagaaacgga agaagaaaga gcatatttga tgtctaagat tatgcctaag ctggtagttg
    12001 gtggtatggc ttgtatgatt tctattgtgg tagctggtct tatggtacca atgttgtaga
    12061 taggtttcat agttaaaaaa gtggtgttaa tttataggag aagctaaaaa gtagtattaa
    12121 tttataaaat aattttatat tagttaattt gttataagaa tattttcttt ttataattat
    12181 aattttgatt ttataagaga gcagattttt tatttgctat gctaatagtt ataatttgtt
    12241 tttatttgct aaaatgcaac tttaatttga tttatgtata atatagggta tttaataata
    12301 tttttgaaga atttttataa aaaattaaaa agaggagaaa aaagggagtg ccacaaagtg
    12361 aactttttag ttctaaggca ctcttttttg tacgaaatcg aatatatttt caacgtttta
    12421 aaagtgagaa agagacttat ctctagtttg agatagccaa ttttaattat taaaattatt
    12481 ttttgtttta tttatcaaag agtatccgtg ttttagaagt tagtattttt tattttatat
    12541 atacaaggtt ttccgtttct aatgttttcc tttttaaaat aattagggta ttcaattata
    12601 gtaaaaggtg gatggtattt tggagtgata ttatttaaag cacagtattg aatgttgaaa
    12661 tcctcacatt ttagagtatt atcttttgta atcaaaaaaa tcttctatac ttatagcaag
    12721 gttatttctg caaatattcc atacataata atatcccaat ttaaattttt tttatataga
    12781 gtttacctct agtctacact cttgaataac tctttcctct atccaaattc ttataaattc
    12841 atcccattta ttactatcta tttattacta tctaaatttt ttagtatgtt tgtaattaga
    12901 aatggattta ttcctttatg ttttttataa atagtttata acttaattta tcatcaaaca
    12961 aatcattcat acaggattac tttttctttt agatattcaa ttcttacttt taataaatat
    13021 ttaatacatt ctatcttatg tggttttaaa aagtgtttat ggctattaac aatctctaat
    13081 atttcattca tatttatttt gtcatagtaa tattttacat cttttatatt gacaaaaaag
    13141 tgctctaagt gtatagtgtg tatataacaa catcacactg aagatgataa aaagggggtg
    13201 ataaaaaatg aaagtgctca tttctaacac atcagataag ccattatacc aacaaattaa
    13261 agagcaaatt aaagaagcaa tatttactga agaactgaaa gaaggggatg caatgccatc
    13321 cataagaaat tttgctaatg atttaaaagt tagtattttg actattagac gagtgtatga
    13381 agaacttgaa aatgaaggct ttcttacacg ccaagttgga ataggaacat ttatttcaac
    13441 tggaaattta gaaatactaa gagactcaaa gagacgtata gttgaacaaa aaatggcaga
    13501 tatgattaag gatgcaaaag cactaggaat tacacagata gagttacaag aaatgatgga
    13561 tattctatat caggaggatt aatatggaaa atattttaga agtttataac ttaaataaga
    13621 cttataccga tttttcatta tctaatataa gctttgcatt accagaagat tgtataactg
    13681 gatttatagg tgttaatgga gcaggaaaaa caacaacaat ccgttcgata ttggatgtcg
    13741 taaagcgtga tagtggaaaa ataaaaatct ttggtaaaga tttcgatgga aatgagaagg
    13801 aattaaaaaa tcgaattgga gttgtatttg atgattcata tttctatgag gagttgtcaa
    13861 tggcagaaat gaaaagtatt attgcacctt catatgataa ttggtgtgaa gaagattatc
    13921 aacaaaatat gaagcgtttt aatcttaacc caaaacaaaa gattgataca ctttcaaaag
    13981 gaatgaaaat gaaatttgct ttagcattag cattatcgca taaagctgag tttttaatta
    14041 tggatgagcc tacaagtggc ttagacccac taatccgtag tcagatactt gacatactaa
    14101 cagaatatat gaagaaatgt ggtcgtggtg ttttcttttc cactcatata acatctgatt
    14161 tagacaaaat agcagatatg attgttatga ttgacaatgg acgtattata ttagagcgtg
    14221 aaaaagatac tctattggac gaacatagaa aagtaaaagg agatattcaa tttctaaatg
    14281 atgaaaccaa aaaattattt ttaaatttag aaattaccgg ctatggtttt atagggatta
    14341 cctcaaaaat taatgaagtg caaaaaaata tgccagatat tttaatagag cgtcctacaa
    14401 ttgaagatat tatgcttgca tatattgaaa ggagagagta attatgttga ttcatttagt
    14461 taaaaaagat attctgattg taaaaaaata tgtattgttg atattattta ttgcaatagg
    14521 aatcccatta tttatattgt ggcgtttacc tccatttgca caaatattgg gatttgtact
    14581 atccattata ttttcagaat ttatgctgtg ccagtatcta ttattaaagg agagtcaata
    14641 ttcaaaagca tctgtattac tttgttcaac tccttatcct agacgtggct tagttcaatc
    14701 taagtatgtt ttatttgcaa tggtatttat ctattgtaca ttagtttact ggattgaaaa
    14761 tttacttatt ccacaaatag gaacttttga cttaacatat attttgattg ttttattaat
    14821 aagttctatt atttatggag tttatatgcc tattcaatat aaattaggat atgaaacaac
    14881 aaaattcttt ttcgtggtga taatcatggc ttcatcattt gtattaccat taactatagc
    14941 tcacagcaat tttgaaattc catttatata tcagtatcca tccatgatag tgaatattgt
    15001 attgttcctt ttaagtttaa ttatactgtt agcttcaatg tttatttcta ttaaaatttt
    15061 tagtaataag gagcttgtat aaggtaaggg ggacttttat atgccacatt taccagattg
    15121 ggctgtttta ctttgtatct tacttccaat agtatataaa gcttgtcaga tatttaaaag
    15181 taaaaaatag agtatatatt tatattgcca tttttattga gtgaagttag atggtatgaa
    15241 aaggttactt gaaaataaag ttacttggtg tgggagagta acatcataat attatagatt
    15301 atatgtttct tgtgaatcaa tttgtatttt ttaataggga atatccagta attttgtttt
    15361 ccattgatta tttttcaatt atgagtatga ttgtgtgtat tgtataccat cttttaaggt
    15421 gtttttaaaa aattatatcg tcaaaatata aatctaagga atcctataaa taaagctttt
    15481 taattttaga aaactaataa aaagtaggac gttaataaaa agaagctgtc tcaaaataga
    15541 gataagcttc tttttcattg ctgaaatgac gaaaatataa ttgatttaat ataaaaaaga
    15601 gtgctttatg aactatttat tttgaacagt agatacactt gcactcaatt tagttactcc
    15661 taagctttat gaactaaaag tttattttga agcttatttt tattaaaaga gattttattt
    15721 ttctaaataa tcatctatat acagtattgc gtttctttta tttattcata atttattttc
    15781 ttat
//
LOCUS       DAGSRM010000091        15755 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.091, whole genome shotgun sequence.
ACCESSION   DAGSRM010000091 DAGSRM010000000
VERSION     DAGSRM010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15755
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.091"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            112..624
                     /locus_tag="MJE33_002949"
     CDS             112..624
                     /locus_tag="MJE33_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBY9648871.1"
                     /translation="MSDFKEILAEEIKNNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QIGFNIEHLNDTPYFKESKMAIICKKMYSQKLEPQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLVKED"
     gene            complement(779..1840)
                     /locus_tag="MJE33_002950"
     CDS             complement(779..1840)
                     /locus_tag="MJE33_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HBY9648872.1"
                     /translation="MIIKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVI
                     SGVQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEA
                     VKAGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIV
                     VEGPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGRNIPVVAAGGITSS
                     SDVKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAI
                     RNKFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAY
                     KIDKLSTVEDVINELISEL"
     gene            complement(1964..2857)
                     /locus_tag="MJE33_002951"
     CDS             complement(1964..2857)
                     /locus_tag="MJE33_002951"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBY9648873.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIESGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPAGIKIKIQSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGNMIVSVTDNENIHYDIIKIPEELK
                     FCAMIPNFKLSTEKARGVLPKEIPYSDGVFNVSRVALLISALLNKNFDLLKVACQDKL
                     HQDYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTIMSLIKENDDSFVDNMKNYLQKL
                     KSDWEIKELCCDSNGAVLNII"
     gene            complement(2867..4348)
                     /locus_tag="MJE33_002952"
     CDS             complement(2867..4348)
                     /locus_tag="MJE33_002952"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HBY9648874.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGISNDGGLYVPSEFPNVKNELIN
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKEFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVSNSDINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLSDKGVYKISDDMEKNLASFYGEYSNEEEVKSTINNVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKIDIPKPIKNLKNAEILHKDIYEKDELKLAIKKFLKV"
     misc_binding    complement(4456..4687)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5489..6397
                     /gene="trxB"
                     /locus_tag="MJE33_002953"
     CDS             5489..6397
                     /gene="trxB"
                     /locus_tag="MJE33_002953"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBY9648875.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEGKVKVVKTSSGEHKAKA
                     VILANGVERRKLGCAGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARENIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCTTSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
     gene            complement(6487..8310)
                     /gene="typA"
                     /locus_tag="MJE33_002954"
     CDS             complement(6487..8310)
                     /gene="typA"
                     /locus_tag="MJE33_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBY9648876.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEDLSPLFKTIVNHVEAYPNYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            8522..8764
                     /locus_tag="MJE33_002955"
     CDS             8522..8764
                     /locus_tag="MJE33_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648877.1"
                     /translation="MLEVTKIIIKYFASESDTFVKEEVCNFEELLKWSHKFIEGKKIE
                     DVLYIKSKENYKVNVIKSLEIKELEIVRKQQIPPYM"
     gene            8912..9103
                     /locus_tag="MJE33_002956"
     CDS             8912..9103
                     /locus_tag="MJE33_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HBY9648878.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
     gene            9387..11888
                     /locus_tag="MJE33_002957"
     CDS             9387..11888
                     /locus_tag="MJE33_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBY9648879.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQSVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYDPSKVKLSQIKAAIEKAGYKPIEEVKNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMTNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKSLFSLSPNMDSLVAIGTLAAFLYSLYTTLQIANGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAIVLVDGKEVETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGKPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINDESILLGNRKLMNDNNIKLGDLEEKSNILAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNAKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAAAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            12048..12716
                     /locus_tag="MJE33_002958"
     CDS             12048..12716
                     /locus_tag="MJE33_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY9648880.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
     gene            12700..13977
                     /locus_tag="MJE33_002959"
     CDS             12700..13977
                     /locus_tag="MJE33_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBY9648881.1"
                     /translation="MRRIFDKWEKLSIKYKLFSITTSLLIALALIIYLILYFLLPSYY
                     HEYKIESLQESLKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDNQGKIVYGKNEV
                     VILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYI
                     ISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQ
                     IEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISEL
                     LNRLVKRQVFLIEEKHMKTILKIEENLEIKAEQERITKAINNIINNAIKYSPEESELI
                     IRLYDKNKRISKKTSNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGL
                     GLYIVSQIFKSHGFDYSIKNKENSVVFTVEFKN"
     gene            14378..14599
                     /locus_tag="MJE33_002960"
     CDS             14378..14599
                     /locus_tag="MJE33_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648882.1"
                     /translation="MDRLKRDMKNMKEDLKEGVKKTAYVMKNTAEDLKDDAQGAMVNL
                     EMKKDEMMERYEQKKFSKELEKQMKNEMK"
     gene            complement(14888..15652)
                     /locus_tag="MJE33_002961"
     CDS             complement(14888..15652)
                     /locus_tag="MJE33_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBY9648883.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSKPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISSIITEKTPTPDIVDILSEFNVNVL"
BASE COUNT         5997 a         2014 c         2360 g         5384 t
ORIGIN      
        1 tgcaataata tatttaaaaa agtattttat tttcaaaact agacttcatt tataatttat
       61 cacgaaaata aaaggaatat aaatataaga gaatgagagg ttattagaga tatgagtgat
      121 tttaaagaaa ttttggcaga agagattaaa aataatccat ttggattgat aggtaaagat
      181 tggacactta taacagcaga aaatgaaggt aaagtgaata caatgactgc tagctgggga
      241 gggcttggag ttatgtgggg aaaagatgtt gcttttgtag taataagacc acagcgttat
      301 actaaggaat ttatagataa tacagataaa ttttctctta cattttttga tgaagatttt
      361 cgtaaagagc tttcatattg tggaaaggta tctggtagag aagaggataa gatatcacag
      421 attggattta atatagaaca cttaaatgat acaccttatt ttaaagaatc taaaatggct
      481 attatatgca aaaaaatgta tagtcagaaa cttgaacctc aatgttttat tgctgaaggt
      541 atagatggaa gatggtatcc acaaaaagat tatcatactt tatatatagc tgaaataacc
      601 aatgtacttg taaaagaaga ttagttataa gggttaatac tatctctaat taaattaaaa
      661 tttcaataaa aggaaattga cacaaaaaag ctaaggacat aaatatgttt ataaagatta
      721 ataaataacg tatttaaact ccttagcttg ataaaatctt aatttagttt tctttaaatt
      781 ataattcaga aatcaattca ttgataacat cttcaacagt acttaattta tcaattttat
      841 aggcatcatt tccacaaaat aaaagtgaat tttcaacatc tcctttaact gcattgatta
      901 gagcttgaga aatacagtaa ggagtttctt ttggattaca aggaatcaaa caattatagc
      961 acttctttat ttctggacga tttattttaa cttcttctat aaatttgttt cttatggcgc
     1021 gtccaggaag acctacagga cttttaacta tatgaacatc ttcttcacta gcatttatgt
     1081 atgccatttt aaagttctca tgggcatcac attcgtaagt agcaacaaat cttgtaccaa
     1141 cttgtacacc acttgcaccc atatctatat attttttaac atcactacta gaagttatac
     1201 caccagcagc aactactggt atgtttctac caaatttttc tccataagta tttgcaacct
     1261 ttaaaatttc aacaaattcc ttatcatagt ctatagaatc tatgttatca agttcctcgt
     1321 tagagtatcc aaggtgacca ccagcttttg gaccttcaac aactattaag tcagcagtag
     1381 ttttctcttt tctatcccac atttttaaaa ttacattagc agcttttgca gttgaaacta
     1441 tgggtgctat ttttacatta cttcccttta ctaatgatgg tagcttatta ggtaatccag
     1501 ctccagatat tataaggtct acaccagctt taacagcttc ctttacgtat gtttcatatt
     1561 ctttcatagc taccataaaa ttaataccaa taataccacc atttgagatt tctttggcct
     1621 tagaaatatg tttttttata gctcttaaat ttgcatttat agtattagtt tcaaaatctt
     1681 cctcatcata tccaatttga actccagaaa ttacaccaac accaccaagc ttagcaactg
     1741 caccagctag tgatgaccta gatataccaa cgcccattcc accttgaatt ataggaactt
     1801 ttgcaactaa atctctaatt attaactctt ttatgataat ccctcctcaa tgtaaacatg
     1861 tttaaagaac tagattataa tacttaatat tattacctag tcctaattat tatactacaa
     1921 attataataa aagagtagtt atattttgta tagaagacga taattatatt atatttaaaa
     1981 cagctccatt agaatcgcaa cataactctt ttatttccca atcagatttt agtttttgta
     2041 ggtaattttt catattatcc acaaaactat catcattttc ttttattaaa gacattatag
     2101 taggtccagc tccacttaaa aatactccaa tactatttaa ttgttcagat ttttcaacaa
     2161 tatcattgta gttctcaatt agagttcctc tataatcttg atgcaatttg tcttgacaag
     2221 ctactttaag taggtcaaag tttttgttta gtaaggcaga tattagtaaa gcaactctac
     2281 taacattgaa taccccatca gaatatggta tttcctttgg taaaacacct ctagcttttt
     2341 ctgttgataa cttaaaatta ggtatcattg cacaaaattt aagttcttca ggaattttta
     2401 taatatcata atgtatattc tcattatcag taacagatac aatcatattt cctaaaatag
     2461 caggagcaac attatctggg tgaccttcta tttctgaagc tatatttagt aattgatttt
     2521 tatctaaatt agcacctgat agagcatttg cagctataac tccagctact atacaagagg
     2581 cactactgcc aagacctcta catataggta cttcactttg gatttttatt ttgataccag
     2641 caggtatttt ttctggttta actctatcaa aaaagtactt catagatgta taaactaagt
     2701 tgttttcgtt tttgtaagca tcttcacaac cttctatttc aagaccactt tcaatttctt
     2761 caacataaaa tttattgtat atatttaacg caattccaag acaatcaaat cctggaccta
     2821 tattagcact tgttgcagga actataatct ctaacataat aatcacctat acctttaaga
     2881 atttttttat agctagtttt aattcatctt tttcataaat atctttatgt agtatttctg
     2941 catttttaag atttttaata ggttttggaa tatctatttt agaaatactt gataatctat
     3001 caattatagc aaagtcatca aggtctttaa aatcacaatc tatagacttt agaacatctt
     3061 cagaaaactt gaatggactt gcagtactta caataacagt ttttgtgtta tcagaagttt
     3121 cttttttata tttttcataa caattgtatg caacagcagt atgagtatct atcaaataat
     3181 tataattttt aaacacattg tttatcgtgc ttttaacttc ttcttcattt gaatattctc
     3241 catagaatga ggctaaattt ttttccatat catcacttat tttgtagact cctttatcac
     3301 taaggtctga taaaagctta tttacaatat cagtatttct atttgatatt tcaaacagta
     3361 atcgttctaa attacttgaa attaatatat ccatagaagg agaagtagtt aatttcaatg
     3421 ttctattttt atcatataca ccagttttaa agaaatcata aagaacatta ttatcatttg
     3481 atgcacatat taatttgttt attggaagac ccatttgttt ggcataatat ccagctagaa
     3541 tattaccaaa attaccagtt gggacaacaa aatttatttt atcatttaaa tttatatcac
     3601 tattgctaac taatttcata tatgaataaa aatagtatgc aacttgtgga agaagtcttc
     3661 ctatattaat tgaatttgca gaagataata tataattatt atccaatagt ttttgattaa
     3721 attctttatc agaaaaaatc ttctttacac ctgattgtgc atcatcaaaa tttcctttta
     3781 taccaactac atatgtattt tttccagttt gagttttcat ttgaagtttt tgtactgggc
     3841 ttacaccatc ttctgggaag aatacaatta tctttatttt atctatatct ttaaatccct
     3901 caagtgcagc tttacctgta tctccagatg tagcagtaag aatcacaaca tctttctcta
     3961 aattatcttt ctttatagat gttttaagaa gatgaggcat tatagtaagt gccatatctt
     4021 taaatgctaa agttggaccg tgatatagtt ctaagaaata agtatcattt actttattta
     4081 atggagctat actactacaa tcaaactttt catcatatgc attttctata caatttttaa
     4141 tttcatcttc agtaaaatca cataaaaatt tactaagtac aaaaaatgct atctgagaat
     4201 acgttaaatt tactaaattt ataagttcat tttttacatt gggaaattca cttggaacat
     4261 aaagaccacc atcatttgat attcctttta ttattgcttg tgatgcagtt actttttctt
     4321 cggtgcctct cgtacttgaa taatacattt tttataatcc tccatatatt ttaaattttt
     4381 aattagctaa ttaaattaga tacagaatat tttaaaaact gaatttaggt aataaaaaaa
     4441 gaccctcatc cctaaaaaag ggacgagaat ctgtttcccg cggttccacc caaattgatt
     4501 ttaaaaaatc cacttgatac tttaacgcaa gtaataaaaa cgtctattcc tactaaaatc
     4561 attaagataa cttcggatag aagctcctag gtagttttca atccttctaa ttagggaaac
     4621 atttcagcta gtagtttcct tctctggtaa tcgattcaga tttactcttc ctaatcaatg
     4681 cctttcaata ttcttaatat tattaattat tctagtttat aaaacagaca aagtcaagta
     4741 ttaatataaa aatttggaaa aaatattttt taaattgtaa aaaaattgta atcaaattga
     4801 gtattagaaa aatatttttt taactttttt aaaagtttaa aaacctagat tttcatagtg
     4861 atgaagctgt ttttaatgaa agtaattaaa agataactat taaatatctg taatattaca
     4921 attttatact acactatatt acaaaaaaag tttacaaaac tttaatttga aaaatgtaat
     4981 tatatggtat atcatataaa tataagtaaa tagtgctaat atttaaacta ttaatcataa
     5041 ttggttcaac aaaagtatat agttaataat gtttaatttg aagaaattgt tagtgtgaaa
     5101 ttacatatat attcaaatta aattcataaa tatttcacat aataatagta tactgtaaac
     5161 aaaaggaggt attaaatatg ggaatagtac ttataacagc agcattagtt ttggttatgt
     5221 atggactttc aattctatca tatatatcag aaagcaatat attagaagac actgtaaatg
     5281 ataaccaata aggttttgaa ttatataaat aagtgagaac tgtaaataaa agttaatttt
     5341 tatttgcagt ttttttatat atatgtaaaa taaagttaat taataaaaaa taatgttttg
     5401 tttatagtat atatggataa taatagtaaa gtataagaat gatgagaata atatataaga
     5461 aataataata taaagagagg taaacgttat ggtagatatc attgtaattg gtgctggacc
     5521 agcaggttta acttcagcaa tttatgctat gagagcaggg ttaagcgtta cagtatttga
     5581 aaaaaatatt tatggaggtc aagttgcaag tacctctgag gtagaaaatt atccagcagt
     5641 tcaaaagatt tctggggtag aattttctaa taatatatac aatcaagcag ttgctcaagg
     5701 tgttgatatt cagtttgatg aggttgaaga gataaattta gagggaaaag taaaggttgt
     5761 aaaaacttct tcaggtgaac ataaagcaaa ggctgttatt cttgcaaatg gagttgaaag
     5821 acgtaaatta ggttgtgcag gggaacaaga atttacaggt agaggagtat cttactgtgc
     5881 aacatgtgat ggagcctttt ttaaagataa agaagttgct atagtaggtg gaggaaatac
     5941 tgcactagaa gatgctttat ttttagctaa taattgtaca aaagtgtatt taattcacag
     6001 aagagatagt tttagaggtg aagaagtgtt ggagaaatct gtgaaagcaa gagagaatat
     6061 tgagatactt tacagtcatg gtgtagaaaa aatagaagga gaaaagactg tatcgaaaat
     6121 agaagtaaaa aacttaaaga ctgaagaaaa aagaactata gatgttagtg gtatttttat
     6181 tgctataggt cttaaaccta ataataaaat gtttgaaaat gttcttgatt tggatgaagg
     6241 tggatatata atttcagatg agtcttgtac aacatcagta gaaggtgtat atgtagcagg
     6301 agatagtaga actaagtttt tacgtcaaat aataacagca gcatcagatg gagctattgc
     6361 agccgttcaa gcagctaatt atataaatgt tgaataatat aaatttcaaa taaaaatagc
     6421 catataaact gaaataattt ttcagtacta tatggctatt ttgttaatta gaggtaaatg
     6481 tacgttttat ttaccttgtc tagatttgtt atatcttaat ctatcatgtt catttaagaa
     6541 tctttttcta agtcttatag aatcaggtgt aatttcaact aattcatcat cttctataaa
     6601 ctctagtgct tcttcaagag taaatattct aggaggagat aattttatag catcatctga
     6661 accagaagca cgtacgttac tcattttctt gtttttacaa ggattgacta ccatatcatc
     6721 ttttctggaa ttcataccaa ttatcatacc ttcgtaaact tctacaccag ggtctacaaa
     6781 cattacagct ctatcactta aggcattaag agaatacccc atagtaacac caggtccttg
     6841 tgctattaaa acaccatttc ctcttgaagg tatttctcct ttaaattctt caaatttttc
     6901 aaatgaacga actaaagtac cttctccacg agtagcgttt ataaattcac ttcgatatcc
     6961 taaaagacca cgagtaggtg ctaagaactc tatttttaca tagtctcctt ctattgacat
     7021 agactccatc ataccttttc tcatgtttaa ttcatttata atagtaccag agtaaacttc
     7081 aggacagttt acaacaactc tttctattgg ttccattagt tttccatctt ctttatgcat
     7141 taaaacttca ggtttagaaa caccaacttc ataaccttca cgtctcatat tttcaagtag
     7201 tatagataag tgaagctctc cacgtccaga aactttgtat ccatcagtag tatcaagagg
     7261 ttcaacttta agaccaacat ttacttcaag ttccttttca agtctatctt tcaaatgtct
     7321 agttgtaacg aattttccac tttttccaac gaatggagag tcgtttacta agaagttcat
     7381 agaaagagta ggctcttcta tttttatcat ttccataggt agtggactat ctaaatcgca
     7441 tatagtttca cctatagata tatcaggaat accagctata acaactattt ctccactagt
     7501 agcttcatca acttcaactt gttttaaacc ttcatacaca gaaagttttg atacttttcc
     7561 tttagataca acagaatctt ctctacatat agcaacttgt gtattgtttt taagagtacc
     7621 tttgtaaatt ctacctatac caagtctacc tacataatca tcatatgcaa gagctgatat
     7681 ttggaactgt agaggttcat tatcgtaatt aggataagct tcaacgtgat ttacaatagt
     7741 tttaaataga ggagaaaggt cttcactatc atcatccatt tcaagtttag caataccttg
     7801 tttagcaata ccatatatta taggaaattc gcattgctca tctgttgcat ttaagtctac
     7861 aaataaatcg aaaacttcat taactacttc ttccgctctc tggtcttttt tatcaatctt
     7921 gtttataaat aatattggtt ttaaaccgaa ctctaaagat ttttgtaaaa cgaatctagt
     7981 ttgaggcatt gggccttcac tagcatcaac taatagaata actgtgtcaa cagttttcat
     8041 aacacgttca acttcagaag aaaagtcact gtgtcctgga gtatcaacta tgtttatttt
     8101 gtaatcttca tagtttattg cacagttttt tgaatatata gttatgcctc tttctttttc
     8161 aaggtcgtta ctgtccataa cgcagtcttt aactacttcg ttctttctaa aaacaccgct
     8221 ttgagaaagg aaagcatcaa ccaatgttga ttttccagcg tcaacgtggg ctataactgc
     8281 gatatttata attttatgtt tttgtgacat ttttgttgct cccttcgatt aaaataactt
     8341 attaattata gcacaatata ttattaggta caaggaggaa tatattaaat tgtattaata
     8401 tagtttaaat tgaataaaac tgttagtttt gcatctgaaa attattttgt ttaaaaaaat
     8461 tttttagaca aataataacc ttaatagata aaaagattaa taaaagagta aaggatgata
     8521 tatgttggaa gtaaccaaaa taataattaa atattttgca agtgaaagtg atacttttgt
     8581 aaaagaagaa gtttgtaatt ttgaagaact tcttaagtgg tctcataaat ttattgaagg
     8641 taaaaagata gaagatgttt tgtatataaa gtctaaagaa aactataaag taaatgtgat
     8701 taagtcttta gaaattaagg aattggaaat agtgagaaaa caacagatac caccgtacat
     8761 gtagtattaa taatatataa aattaatatg taatactcgc aataggatat aaatggtata
     8821 tatggtaata atttatatta aaaaaacttt tataaagttt atatttgggt ataaaagtat
     8881 taattagaca ttaattagga ggaacttaaa tatgataaga gtatgtccaa actgttcaaa
     8941 tgtggatgta aataagctaa aaactttggt tggagaagaa aatgtaaaga ctggttgtat
     9001 aggtcagtgt agagcattta aaaaagaagc tgttggaata atagacaatg agcttgaaat
     9061 aaaggaaaca gaagcagagt tttttgaagc atgtaaagaa taaattttaa ataaaatata
     9121 ataaatagag catctaaatt gtataagcat taaacaattt agatgctcta tttttatata
     9181 tacatttaat tatgagtata cttttctatt aaatacaaat aaatataact aatacattta
     9241 tttattaaaa aaatattgat ttaatatacc tatagggggt ataataaaaa tatagatttc
     9301 taaataagta atttatcaat attacatatc aaaataaaac gttacaaatt agctgaaaat
     9361 atattgaaag aaaggaagat tttattatga gttcaaataa agtggtagaa agttataaaa
     9421 ttacaggtat gacatgtgca gcttgtgcaa aggcagtaga gagagtaact aagaagatgg
     9481 atggagtata tgaccaaagt gtaaatatag ctactgaaaa acttaaaata gaatatgata
     9541 attctaaggt taattttgat gatataaagc aggttgtaga aaaagcagga tatggaatta
     9601 taaaggaaga aagtaataaa aaaattgata tgaaaattga tggaatgaca tgtgcagctt
     9661 gtgcaaaagc tgtagagaga gtggtaaaaa aattggatgg agtagaaagt attagtgtga
     9721 atattgcaac agataaagct aatatagact atgacccatc aaaagtaaaa ttatctcaaa
     9781 taaaagcagc tattgaaaaa gctggatata aaccaataga agaggtaaaa aataaggttg
     9841 atgtggatga agataaattg agaaaagaaa gagaaatgaa gtctttattt gttaaattta
     9901 tagttgcaat agtatttgca gtacctctgt tttatatagc aatgggacct atgataataa
     9961 agcctatagg gccatggcct ttgccagaaa ttataaatcc tatgacaaat acttttaatt
    10021 atgcattaat tcagttaatt ttagtaatac cagtaatgat agctggttat aaattttata
    10081 taaatgggtt taaatcactt ttttctttaa gccctaatat ggattcttta gttgcaatag
    10141 gaacattagc cgcattttta tatagtctat atacgacact acaaatagcc aatggacaaa
    10201 ttcaaggtat gcaccatcat cagttgtatt atgaaagtgc aggaataata atagcactta
    10261 tcttacttgg taaatattta gaatcaaaat caaaaggaaa aacatcagaa gcaattaaaa
    10321 agcttatggg gctacaacct aaaactgcaa tagtgttagt tgatggtaaa gaagttgaaa
    10381 ctcctataga agaagtagag ataggagata tactattagt aaaaccagga actaaaatac
    10441 ctgtggatgg agttgtaata gaaggatata catcagttga tgaatcaatg cttacaggtg
    10501 aaagtatacc agtagaaaag aatgttggaa gtaaggtgac aggagctagt attaataaaa
    10561 atggtgttat taagtttaag gctgaaaaaa ttggtggaga tactgctctt gcccaaataa
    10621 taaagttagt tgaagatgca caaggtacaa aagcacctat tgctaaactt gccgatactg
    10681 tatctggata ttttgtacca atagtaattg caatagctgt agttgcatca ttattgtggt
    10741 ttttaatagg aggaaaagat atagtatttg tacttactat atttatatca gtacttgtaa
    10801 ttgcttgtcc ttgtgcatta ggattagcaa caccaactgc tataatggtt gggactggaa
    10861 agggcgcaga aaatggaatc ttgataaaag gtggagaggc attagaatct gcacataaag
    10921 taaatacagt gatatttgat aaaacaggta ctataacaga aggaaagcca aaagtaacag
    10981 atatagtatt aaataataat gtaaaagaag aatatcttat caagattgct tcaagtgcag
    11041 aaaaaggttc agaacatcct cttggagaag caatagttaa gtatggagaa gagaagaata
    11101 taaaatttga aaaagttgat aattttaaag ccataccagg tgctggtata caagttacta
    11161 ttaatgatga aagtatatta cttggaaata gaaagcttat gaatgacaat aatataaagc
    11221 ttggggattt agaagaaaaa tcaaatatat tggcaagcca aggtaaaaca cctatgtata
    11281 tagctgtaga tggaaattta tcaggtataa tagcagttgc agatgtagtc aaagaaagta
    11341 gtaaaaaagc aatagaaata cttcatgata tgggaataaa agttgcaatg gtaactggag
    11401 ataatgcgaa aactgcaaat gctatagcaa atcaagtcgg tatagatatg gttttggcag
    11461 aggttttacc agaagataag tcaaaagaag ttgaaaaact acaaaatcaa ggaaagtttg
    11521 ttgcaatggt aggggatgga ataaatgatg ccccagctct tgcaaaagca gatataggga
    11581 tagcaatagg aagtggaacg gatgtagcca ttgaatctgc ggatatagta cttatgaaga
    11641 gtgatttaat tgatgttcca acagcaatta aacttagtca tgaaactata aaaaatataa
    11701 aacaaaacct attttgggca tttggatata atacaattgg tatacctgta gcagcaggaa
    11761 tattatatgt atttggagga cctttattaa acccaatgat tgcagcagca gcaatgagct
    11821 taagttcagt ttcagtcgta tcaaatgcac ttagacttaa aaacttcaag gcatataaga
    11881 gagattagat taataaatta aataaaatgt atccaaaaga gctatcttta aattaaaagg
    11941 attaatttac aaattaagat agctcttttt tcatgtaaaa ttcacaacaa taagataaaa
    12001 tataagtaat atacgaaatt ttaaataaaa tagaaaggat tgatagaatg aattcgtcaa
    12061 tacttgtaat tgaagatgat tcaaatatac aagagttaat atcagaattt ttaagtgcag
    12121 aaggttatca agttgataca gcaaatgatg ggttggaagg tatacaaaag tttaagcaag
    12181 gaagttatga tttagtaata ttagatataa tgatgccaaa cttagatggt tatggagttt
    12241 gtaaaatgat aagaaagtca tcaagcgttc caattatatt tttaactgct ttaaatgatg
    12301 aaggagacca acttaaaggg tttgacttag aatgtgatga ttatataaca aaaccatttt
    12361 catttaatct tcttataaaa agagtagaag caattttaag gcgaagtaac aagactataa
    12421 atgacaaatt tatagttttt gaaaaattaa aattagattt aaacacatat attgcagaga
    12481 tagatggaga acctatagaa cttacactaa aagagtttaa tatattaaaa gctttgatag
    12541 aaaagtatcc acaagttata actagggaag gtcttttgga tagtatatgg ggttatgact
    12601 attatggaga tactaggatt gttgatgcac atataaaaaa tataagaaaa aaaatatcat
    12661 taccatatat taaaactgta aaaggaatag gatatacact tgagaaggat atttgataag
    12721 tgggaaaagt taagtataaa gtataagtta ttttcaataa cgacatcatt attaatagca
    12781 ttagctttaa taatatatct aattttatat tttttattgc catcatatta tcatgagtat
    12841 aaaattgagt ctcttcaaga aagtttgaaa tctttagtag atagttcaat tcatttcgat
    12901 acatatactt tagaagaaag actttattat atggctaaag accaaaattt ggctatttta
    12961 ttaaaagata accaaggtaa aattgtatat ggtaagaatg aagtagttat tttaagatat
    13021 agtaagtaca tgattaatag tcttgaggat gagtatagaa cttctatacc tatatatact
    13081 aaagatgcaa aagatggtcc atatacttta gaactcgtta tgccattaca accaattgac
    13141 gaagcaaacg aagttataag aaaactaatg ccatacatca tatcaatagc aatattgata
    13201 gcgattattg gagcatatat atattcaatt gtaataacaa agcctcttat aaacataata
    13261 gagagtgaga gagaacagga atatagaagg aaggattttg ttgctacaat atcacatgaa
    13321 ctaaaaactc caataaccat aataagtggt caaattgaag gtatgattta tagtgtaggt
    13381 aagtataagg atagggatac ttatctgaaa aaatcgtatg agtgtacaca agaattaaaa
    13441 gatttagtaa atgaaatgat agaggtttca aaatcagaaa tacttgaaaa agatttgaaa
    13501 ttggtatcaa taaatataag tgaattatta aatagacttg taaagagaca agtattttta
    13561 atagaagaaa aacatatgaa aactatttta aaaatagaag aaaatttgga aattaaagca
    13621 gaacaagaga gaataactaa ggcaatcaat aatataataa ataatgccat aaaatattct
    13681 cctgaagaat cagagcttat aattagactc tatgataaaa ataaacgaat aagtaaaaag
    13741 acttctaatc aaagagtggt tttagaaatt gaaaatactg gtgtaactat cgagaaacgt
    13801 tatttagaag aaatatttaa tccattttat agaatagaaa aatctcgttc gagaaaaact
    13861 ggaggaagtg gacttggact ttatatagtc agccaaattt ttaaaagtca tgggtttgat
    13921 tatagcataa aaaacaaaga aaactctgta gtattcacag tagaatttaa aaattaatta
    13981 cttattataa agaatatata actttcaata atagttcatt ttaaaatcac aaaaaattca
    14041 taatgaaact gtattatata aatataaata agcttaaaac cataaaatac accaactata
    14101 atgatgagat ttccttacat cctccatata agtcatcatt atagtaaccc aacaattaag
    14161 ttactatgaa tttaaaaaat aacaccgagc atataatcta tgcttagtgt tatttttatt
    14221 ttgaaataaa aaatatttaa tttattgaaa tgtatagatt tagatagttt aaactatatt
    14281 taagacttaa aaattataat tgatattaca aataaatata tgtactttgg gaaaaataaa
    14341 tataggaagt attatattta cagaggaggt agttttgatg gatagattaa aaagagatat
    14401 gaaaaatatg aaagaagatt taaaagaagg tgttaaaaag acagcttatg ttatgaaaaa
    14461 tacagctgaa gatttgaaag atgatgcaca aggtgctatg gtaaacttag aaatgaaaaa
    14521 agatgagatg atggaaagat atgaacaaaa gaaattttct aaagaattag agaagcaaat
    14581 gaagaatgaa atgaaataag cttttattta tgaataacaa attattgtat tcatataaaa
    14641 agtagccagt aatattaaat agtttattta aggaaataga ctttctaaaa ataactgtat
    14701 tagatttaga aagtctattt caaattagtt ttataaatag acataaggat aaagtagatg
    14761 ttatagaact agattttaaa aaatagatac taaggataaa ataaatatta tagaaatatg
    14821 tttttaagaa tatttatcaa ataaaaattt atatttataa gaaatcctat aaaacatata
    14881 attaaactta taaaacattt acattaaatt ctgataaaat atctactata tcaggagtag
    14941 gcgttttttc tgtaattata gaactaatat cttctaaatg agcaaacttg aaatttccat
    15001 caaatttaaa ctttttatct tccataacta taaatacttt tttacttgaa tttattatag
    15061 ccttcttcgt aagaccatct tcaatttcaa aagttgtaag tgaagagttg aatacatcta
    15121 ctccacaact tcccataaaa gtcttatcaa acttatattt cgatataaac tctatagttg
    15181 gagtaccgac aaaaccgtct aaactgagat ttaataaacc tccagtggaa attatattta
    15241 aattatttgg ttttgaagta actacattta gtatatccag catatttgta acaagtgtta
    15301 cttttttatt actttcagct aacaactttg caagcaagat gttaattgta gagatatcta
    15361 aaaatatagt ttctctatct tcaattaaat taaatgcttt ttctgctatt attttctttg
    15421 ttggaatatt aatttctttt ctgtcttttg tatcctgatt ctctagtgtt tctctagcta
    15481 gtacagctcc tccataaatt cttcttattg aagaaatctt ttctatattt tttaaatctt
    15541 ttcttataca atcttctgta acattaaatt tagaactgag ctctttgaca gtaactctac
    15601 cttctttttt taggatattt aatatagctt ggattctttc ttctgcaaac attttctacc
    15661 tcttttcgca atatagtatt atagtaaata ttataagata aaatacaata tatcaactat
    15721 tttaacatga aatacaaaat taatttaata taaaa
//
LOCUS       DAGSRM010000092        15403 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.092, whole genome shotgun sequence.
ACCESSION   DAGSRM010000092 DAGSRM010000000
VERSION     DAGSRM010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15403
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.092"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            20..727
                     /locus_tag="MJE33_002962"
     CDS             20..727
                     /locus_tag="MJE33_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9648884.1"
                     /translation="MLKVINVSKSYKNKKVVSSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFEEGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFIADIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            774..2369
                     /locus_tag="MJE33_002963"
     CDS             774..2369
                     /locus_tag="MJE33_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9648885.1"
                     /translation="MSNLSILIKNNIINEFKLNALKKADGREKQKIMGMMLSVVIIAG
                     LLIFYVTSLSLSLVDILKQINQLEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDL
                     LTSLPIKESTVLASKILMLVGANYLFSSICMIIPAVVYFLNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFLFIGQISSRIKYKNLVLIIGSIILMLLYTLLMSRLNSMSVEMLKN
                     SVSISETIRKVYPPIYYFVDALKTGSIVSFLAFIAISIIPFFMFVTLFAKKFKDINAK
                     MSEGYKSKNYEFKGQKTSRPSKALLKKEFKRYTSSFIYFLNSSAGLLFLIVGTILILL
                     FGADKVGELLKLNIDFNLIKIQLLGVFFFIIVMNCTTYCSISLEGKNLWILKSSPIDE
                     REIFWSKILMNFLLNAPLSILCLILVSIRLDFEIQFIIVSICLIGSFSMFVALMGLLS
                     NLLYPNLDWKNEVAVVKRSASMIVTMLVSLAYVIILGGIYVLLGISFLNIYIILASIL
                     TLILDFMIWRIISTKGVKIFKNL"
     gene            2472..3554
                     /locus_tag="MJE33_002964"
     CDS             2472..3554
                     /locus_tag="MJE33_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBY9648886.1"
                     /translation="MRKLSKREKDFIQYGFLLFLICITTYLVLTTLDIKLIPKIIRLV
                     NKTYIFLGILTIIVYMIIESIVTHLIINAVQKTKVKFIGIKMATMGFYYNLVTPFASG
                     SQPMQIYALTKYDVSLSKSVAIVTNKTVVFQSTVTVFCGILILLNKSLLMEQVHSVKV
                     LITFGMIMNVSMLLGGLLIVFSPKQVKMIAEVLINFLSKFKTFKFLDSKRDRINHYID
                     DYNYSIKIFIKNVRILVLSILLTVLQLIAYFSIVYCVYKASNLRGVSYVHIMTLQVFL
                     YMAISPIPTPGNVGANEVTFFTMFSNIIPKELLGYSVLLYSGFVYYFILIVSGLFTFG
                     THYTLNSWKKEDKVNSNLDKVIEKLN"
     gene            3703..3864
                     /locus_tag="MJE33_002965"
     CDS             3703..3864
                     /locus_tag="MJE33_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HBY9648887.1"
                     /translation="MKQDKQNEKQSEISKIKQSNDNKLEIAQELHVSKNIGNMLGYRI
                     DKKKMNRPS"
     gene            4205..5473
                     /locus_tag="MJE33_002966"
     CDS             4205..5473
                     /locus_tag="MJE33_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBY9648888.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            5668..6456
                     /locus_tag="MJE33_002967"
     CDS             5668..6456
                     /locus_tag="MJE33_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBY9648889.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSKLNLDYIKEYEDYVEIGSMTTFRTLETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSLRNAKSDYPILNVSVSKHMEQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASREYRKAMSKVLLKRAIMEVI"
     gene            6456..6905
                     /locus_tag="MJE33_002968"
     CDS             6456..6905
                     /locus_tag="MJE33_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBY9648890.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKVGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRALDKEITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVLAMKDELENPTDDEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            6909..9173
                     /locus_tag="MJE33_002969"
     CDS             6909..9173
                     /locus_tag="MJE33_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBY9648891.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKDALVVKILRSPHAY
                     AKIKDIDTSKAEMVDGVECVLTYKDVPKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAIDEKTAIKAMNLIKVKYEVLTPVLDFEEAIGNKTMVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDVVIEETYYTQAQAHAMMETYRAYNYLDHMGRLVVV
                     SSTQIPFHVKRHLSRALEIPASKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKMDP
                     TDLRLKNVVTEGETTFAYGKTLNSVDLIKCINKGKELIGWNEKYPCKHLDNGKIRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLDTSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGATKLGVEKDEVE
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDTE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMSLFEEVRYTEKGSMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMEMNK"
     gene            9313..9624
                     /locus_tag="MJE33_002970"
     CDS             9313..9624
                     /locus_tag="MJE33_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648892.1"
                     /translation="MAGNLNNMRAVNNFRGDKNILECLVSFEGRSISQRKVRVFFKEK
                     QNQIEIDFAEEEISKLVENVVLNTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGSRV
                     H"
     gene            9910..10410
                     /locus_tag="MJE33_002971"
     CDS             9910..10410
                     /locus_tag="MJE33_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBY9648893.1"
                     /translation="MRKNSINIALTAAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIIFGTLATFISASMIYITRKLGKQNKSTLV
                     IASLWPTVINSIVVGLMLNVFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     VINIKF"
     gene            10467..11453
                     /locus_tag="MJE33_002972"
     CDS             10467..11453
                     /locus_tag="MJE33_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBY9648894.1"
                     /translation="MSKVKRLTDVWIYINDKQKFTIKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMIILFALKSLQIYNYKLMEIEINS
                     IMRKVSYERKSSIKVGIENIKRYIGFVANDTNDGDFKSSNVTEFFRASSESLILNIKY
                     KCGNQILEKNVCPIGIYLNKGAWFSPAYDCKSDKIILVHIDNIIDIKRTGESKKIHIN
                     LEEWLHEVYNNLYGNSSTTKKFGEIIYLNVLLTKEGVSKIKDSSWNLDMELNEDGSGI
                     ISTFIKVEDIDCYASILFFIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
     gene            11503..12225
                     /locus_tag="MJE33_002973"
     CDS             11503..12225
                     /locus_tag="MJE33_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HBY9648895.1"
                     /translation="MENFNKNAKIIGIGAEGINIINEVEEKIKANMDIEKININQEIE
                     KEYVRSLLDGVDILFLIYSSEDKHIRDIIKAVSYMSNERRVLSIGMDCSEKENKEDLE
                     LGREFKINNDSIFKFVDLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKSYSEIADILFDRMEYIGEEFISKKGIVFVEGSPEFSIMELNDLISNIQSKVEESYE
                     VIFSLYIKENLNGNIRVGLLYN"
     gene            12254..14188
                     /locus_tag="MJE33_002974"
     CDS             12254..14188
                     /locus_tag="MJE33_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBY9648896.1"
                     /translation="MSENIRGTEYWLMEEFFYQETDTPQIFREESIIALGFDIKINLK
                     KVFSMGKKSEKYINENCNDKDFIKKFMSIKKGDYFLYKAYQNIEEKVKCNVSIGIVEE
                     DFENGYELSHILGHTLPVKWMHNFDTVSNCKVYTSEFYKLNNNDISSYLEIIKSQDKK
                     LLRKRIEESENNKWYLKNYYYKNVDKCVDDEELFIQPINLEQLESLKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSALEGGYFFPVRWIEYEKENIDGNAWV
                     IFENNSIVEKYVKRFTNKMPLNTILYGPPGTGKTYNTKQMVYNLVSETSLRNRYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKPEDGILKDISIEACYNALKFEKKM
                     SFELEKDSLTLETIKDRKKKLVLENIEYSESFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFSLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPDYDLLSIVDGIDIRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEKLIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
     gene            14181..>15403
                     /locus_tag="MJE33_002975"
     CDS             14181..>15403
                     /locus_tag="MJE33_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HBY9648897.1"
                     /translation="MSKNIYVKETYEWIRIGNGENELTEIEYEKLLKYLENNNDVLKS
                     NIIDIKYKKLRFINYVGIICFENVILEILPKLSLSDNLVKDREILLQMLSICNKIPIT
                     MNEKIRLSLKNYNLLNFFVMYFIESMQTQMKKGIYFEYINKIENLNVMRGKILLSTYA
                     KEKGISPMKIRCEYDEYSENNFLNQVLKKACISILCRINDNSIQGKIKKILSYFQNVD
                     LIYIDRKKLLDYKFYKNNDRFKDCYLLARLLLLNLSMDNSQNNQEAFSILFEINTLYE
                     EYIGILIKSIWDNSFRETYIQDKSKFLLKNEQTGKKNFNLRPDIVLYDLKNEYEIIID
                     TKWKAIEVDSNVFYRSSDIYQMYAYITAYENAKRCILLYPCIQKDKNYSSWKLSESFK
                     GKFIEAKTVRLDDIK"
BASE COUNT         6108 a         1437 c         2675 g         5183 t
ORIGIN      
        1 tataatatgg agggaaatta tgttaaaagt aatcaatgta tctaaatcat ataaaaataa
       61 aaaagtagta agtagtgtct catttgatgt aaatgatgga gagctttttg ggtttatagg
      121 acataatgga gcaggtaaaa ctactactat taaagcaatt gttggaatac acgattttga
      181 agagggagag gtactaataa attctaaatc tataaaaaaa cagcctgttg aatgtaaaaa
      241 ggaaatggca tatatacctg ataatccaga tttatacgag tcattaactg gtatacaata
      301 ccttaatttt atagctgaca tatttgaagt tgccaaagat aaaagagaag agttaataaa
      361 atattattca aataagtttg aactcaataa agcattagga gatttaatat cttcatattc
      421 acatggtatg aaacaaaaat tagctataat ttcggctttg atacacagcc caaaaatctt
      481 aattcttgat gaaccttttg taggtcttga cccaaaagct tcctttatat tgaaggaaat
      541 tatgagggag ttttgtgaca aaggtggttg tatatttttc tcaacacacg ttttagaagt
      601 tgctgaaaaa atttgtgata agattgccat aataaaagat ggaaatataa ttgcttatgg
      661 aactacagaa gaagttaagg gaagtaattc acttgaaagt attttcatgg aattaataga
      721 gaaataaaat gttttttata caatatagtg tctaaagtga ggaatgataa aatatgagca
      781 atctaagtat attaataaaa aataatataa taaatgaatt taaattaaat gcgttaaaaa
      841 aagctgatgg tagagaaaaa caaaaaataa tgggaatgat gctttcagtt gttataatag
      901 cgggtctatt aattttttat gtaacgagct taagtctctc tttagttgat atcttaaagc
      961 aaataaatca actggaaatg ctactgatat ttggttttgg attatcaaca ttaacaacta
     1021 ttgttacatc attatataaa acgtcatcat atctatttca agcaaaagat ttagaccttt
     1081 tgactagttt acctataaaa gaatctactg tactagcaag taagatatta atgttggttg
     1141 gagcaaacta tttattttct tcaatatgta tgattatacc tgcagtggtt tactttttaa
     1201 attctgatgt aagtataact tatttccctt atttgatatt gctattttta gctttaccat
     1261 tattaccgat agttgcttca tcaataattt ttctatttat agggcagata tcatcaagaa
     1321 taaaatacaa aaatttagta ttaatcatag gtagcattat attgatgttg ctttataccc
     1381 tacttatgtc aagacttaat tctatgtcag ttgaaatgct aaagaatagt gtgtctattt
     1441 cagagacgat tagaaaagta tatcctccta tatattattt tgttgatgct cttaaaacag
     1501 gaagtatagt ttcattttta gcatttatag ccatctcaat aataccattt tttatgtttg
     1561 taactctgtt tgcaaaaaaa tttaaagata taaatgctaa gatgagtgaa ggatataagt
     1621 caaaaaatta tgaatttaag ggtcaaaaaa cttcaagacc ttcaaaagct ttattaaaaa
     1681 aagaatttaa gagatatact tcttcgttta tatacttttt aaattcatct gcaggtttgt
     1741 tatttctaat agtaggtaca attttaatat tattatttgg agcagataaa gtgggtgagt
     1801 tactgaaatt gaatatagat tttaatctta taaaaataca attattggga gtattcttct
     1861 ttataatagt aatgaattgt acaacatact gttcaatttc tcttgaagga aaaaacttat
     1921 ggatactgaa atcttctcca atagatgaaa gagaaatatt ctggtcaaaa atattgatga
     1981 attttttatt gaatgcacca ctatctattt tatgtctaat attagtatct ataagattag
     2041 actttgaaat tcagtttatt attgtatcaa tctgtttgat tggaagtttt tcaatgtttg
     2101 tagcattgat gggattatta tcaaatttat tatacccaaa ccttgattgg aaaaatgagg
     2161 tggctgtagt aaaaagaagt gcaagtatga tagttacaat gttagtatca ttagcttatg
     2221 taattatact tggaggtata tatgtattac ttggaatttc gtttttaaat atatatatta
     2281 ttttggcaag tatacttact ttgatattag attttatgat atggagaatt atttctacta
     2341 aaggagtaaa aatatttaaa aatttataag tgtatttatg tcaaataaag acttatcttt
     2401 tttagtcttg caaataatag gttaagtagt atactatgta tagtgatatg aaaaaattta
     2461 ggagaaaata aatgagaaaa ttatccaaaa gagaaaaaga ttttattcaa tatggatttt
     2521 tattattttt gatatgtatt actacttatt tagtattaac tactttagat ataaaactaa
     2581 tacctaaaat tataaggcta gtaaataaga cttatatatt tcttggcata ttgacaatta
     2641 tagtatatat gataattgaa tctattgtaa ctcatttgat aataaatgca gttcaaaaaa
     2701 caaaagtaaa atttattggt attaaaatgg ctacaatggg attttactat aacttagtta
     2761 ctccatttgc atctgggagt caaccaatgc aaatatatgc acttacaaaa tatgatgtaa
     2821 gccttagtaa atcagtggct atagttacta ataaaactgt agtatttcaa agtacagtta
     2881 ctgtattttg tggaatttta attttgctaa ataagtcatt actgatggag caagtacatt
     2941 ctgtgaaagt cttgataact tttggaatga ttatgaatgt aagtatgttg ttaggtggac
     3001 tactaatagt atttagtcca aaacaagtaa aaatgatagc agaagttttg attaattttt
     3061 tatctaaatt taaaacattt aaatttttag attcaaaacg tgatagaata aatcattata
     3121 ttgatgatta taattattct ataaaaatat ttattaaaaa tgtaagaatc ttagttttaa
     3181 gtatattact tactgtgtta caattaatcg catatttcag tatagtttat tgtgtgtata
     3241 aagcctcaaa tttaagagga gtttcatatg tacatataat gacattacag gtctttttat
     3301 atatggctat atcaccaatt ccaacaccag gaaatgttgg agctaatgag gttacatttt
     3361 ttactatgtt ctccaatata attccaaaag aattattggg gtactctgta ctcttatata
     3421 gtgggtttgt atactatttt atattaatag tttcaggttt atttacattt ggaacgcatt
     3481 atacattaaa ttcttggaaa aaggaagata aagtaaattc taatttagat aaggttattg
     3541 aaaaattaaa ttaataaaat aattagggaa attctatata taaatatggt gttgaataag
     3601 ctatattata ttgagatatt tccctttttt gattaaaatt atagaaattt gaaataataa
     3661 gtaataaaca gaaaaagtga tattgttttg aaaggagaaa ctatgaaaca agataaacaa
     3721 aatgaaaaac aaagtgagat ttctaagata aaacaaagca atgataataa acttgaaata
     3781 gctcaagaat tacatgtaag taaaaatata gggaatatgc ttggatatag aatagataag
     3841 aaaaaaatga atagaccaag ttaatgaaaa tgttgaaaat ggtacatttt aaaaaatata
     3901 tataatgaaa tgttttattg ttaaatttaa acttatttta atggaggaaa aagtcgaaaa
     3961 atatctaaaa agatatggat ataaaaaata ttaatatata tatactataa acttgacaaa
     4021 atcaaccttt taataactag caaaaaatag caaaattata taagcgttat ttaattgtca
     4081 aaaatgaaag ctaaattgca tgtcctaaaa aaaatgattg ataacattta tttgtgtgct
     4141 ataattatat agtaaaacaa taagtatatt gcataatgta tgcaaatcgg gaggagagac
     4201 ggacatggaa gcatctatac acacaattgc cagcaataat ttattgataa tttttgctat
     4261 tgtaagtata actggaataa tttgtagtaa gcttagtgaa ataataaagg ttccagatgt
     4321 agtgttatat ttacttgtag gtattttaat aggaccatca tttttaaaat ttatagatat
     4381 tcgtggattt caaatagaaa atcaactaat acttacattt ggttctgcat ttattttata
     4441 tttaggtgga aaagaaataa gtttaaaagt attgagaaat gtgaagatat ctgtattctt
     4501 attagctact ttaggagttg tgatatctgc ttttataatg caacaagtaa taggatttac
     4561 ttttgggata agtgcaatga ctgctttact agcaggaact ataatagcat caacagaccc
     4621 agccactctt gtacctatat ttaatcaggt aaagataaag gatagggtaa aacaaactgt
     4681 aataagtgaa tcagctttta atgatgcaac aggagctata ctaacatcag cagtgttggc
     4741 tgtaattttg tcaaataaat tttcattagg tgaaaatatt tatgaactgg ggacaatggt
     4801 tattgtcgga gttttagttg gtttaattac aggaatactg cttttgaaat tagttaatga
     4861 taaaccttat ggaatattta aagattttgc accaataata tctgttattt cagttgtagt
     4921 tgcatatgaa ttatctacaa aattaggtgg aagtggatat atgtcttgct ttattgtagg
     4981 tataataact ggtaacaaga aaaactttaa aatttggtta agtcaaaaat cttatgatgc
     5041 agatttttat gttgctgaaa ctttaggtac tatttgtcgt atggcaatat ttataatatt
     5101 gggtagccaa gttaatttgg ctcagttaag taaatatttt atgccatctg taattacagt
     5161 attagtatta atgtttatag caagaccact ttgcgtttta gtttgtacac ttgtagacag
     5221 ggaagcaaaa tggagtaaaa gtgaaatatt atttatgatg tgggtaaggg aaacaggcgt
     5281 aataccagca gcactttgtg gtattatttc agcaatgaaa gttcctggat acgaagtgat
     5341 ttcatctgta gtattcatga ctatactaat tacattagta atacaaggaa gtactacaaa
     5401 attagtagct aaaaaacttg gattattaga agaagaagtg ataaatataa gtgaaaaagt
     5461 atcaactttt taaagttttg ctttaaaata gtacctgtta atggctattt taatattgtc
     5521 taatgatgat tattgaaata gtcatttttt tatgtataag taatttacag aatttacata
     5581 ttagacatat ttattatcaa aatttgttga agctatgata taattagttg tatttaaaat
     5641 aagaatagca aataagggag gaaatttatg tttacggtta tggatattgt acagccagat
     5701 acagtagaag aagcgtactc aattttaaat aaaagaaaaa ctaaccaagt aattggtgga
     5761 agtgcttttt taagaatggg gaaaaaaaga ataggaacag gaatagaatt atctaaatta
     5821 aatttagatt atataaaaga gtatgaagat tacgttgaga ttggttctat gactactttt
     5881 agaacattag aaacaagttc cataataaaa aataattttg gaagaataat agaagattca
     5941 gttaaaaata taataggagt tcagtttaga aatgtagtaa cagttggtgc aacagtattt
     6001 tctaaatatg gtttttctga cttgatagta gcattacttt cattagatac agaagtagaa
     6061 ttatataata ttgggagaat tagtcttgaa gagtttctaa atagagatta tgagaaagat
     6121 ttacttatta agatatacat aaagaagaca aataagaatg cttcttataa atcacttaga
     6181 aatgcaaaga gtgattatcc aattctaaat gtatctgtat caaaacatat ggaacaattt
     6241 aagttatgtg taggagcaag accgcagaaa gctacgattg caaaacaagc tagtgaattt
     6301 ctatctaaca atgaaatcaa tgaaattaat atagataaag cagtagaaat agcatcagaa
     6361 gaactaactt ttggttctaa catgagagca tctagggagt atagaaaagc tatgagtaaa
     6421 gtacttttaa aaagagctat aatggaggtt atttaatgtt agtaaagatt aatataaatg
     6481 ggaagaaaaa aagtgttgat atagaaccag aagaatatct ggttgatact ttaagaaaag
     6541 ttggtaattt aagtgtgaaa agaggttgtg acacaggctg ttgtggttta tgtacagttt
     6601 taattgacaa gaaacctaca ttatcctgtg caacactgac attgagagct ttagataaag
     6661 aaataactac aattgaaggt ttagaaaatg aagttaaaga atttagtgaa gttttggtaa
     6721 aagaaggagc tgagcaatgt ggtttctgtt ctcctggctt tatacttact gtacttgcaa
     6781 tgaaggatga gcttgaaaat cctacagatg atgaaataaa gcattatctt acaggaaatc
     6841 tatgtagatg tacagggtat atgggacagt taagagctat caaaacttat ctggaggtaa
     6901 aatagaacat gaatatagtt ggaaaaggaa tcaagaaaat agatggaatg agtatcacaa
     6961 caggaaagcc tgtatataca gatgatttat cagcaaaaga tgctttggtg gtaaagatac
     7021 ttagaagtcc tcacgcttat gcaaaaataa aagatataga tacatcaaag gcagaaatgg
     7081 ttgatggtgt tgaatgtgtt ttaacatata aagatgtacc aaaatctcga tttacacttg
     7141 ctggtcaaac atatccagag ccatctccat atgatagatt aattcttgaa gatgttgtaa
     7201 gatatgtagg tgacgaagta gctatagtgg cagctataga tgaaaaaact gcaattaaag
     7261 caatgaatct tatcaaagta aagtatgaag tacttacacc tgttttggat tttgaagagg
     7321 caataggaaa taaaactatg gtacatgatg aagatagaca ttgtaacttt gatataggta
     7381 tgcaaagaga aaaaaatata gtatcccatc atgattatac aaaaggtgat gtagaaaaag
     7441 tattaagtaa ttgtgatgta gtgattgaag aaacttatta tactcaagct caagcacatg
     7501 caatgatgga gacgtatagg gcatacaatt atttagacca tatgggaaga ttggttgtag
     7561 taagttcaac tcaaatacca tttcatgtaa aaagacattt atctcgtgca ctagaaatac
     7621 cagctagcaa aattagggtt ataaaaccta gaattggtgg cggatttggt ggtaaacaaa
     7681 ctgcatgtgt tgagattttt tctgcaattg ttactttaaa aacagggaaa ccagctaaaa
     7741 taatttatga tagaaaagaa acttttaatt gttcaacaag tagacatgca atgagattaa
     7801 aagttagact aggtgctaca aatgatggca taataaaggc aatagatata aaagcacttt
     7861 ctgatacagg agcatatgga gagcatgctt ctacaactat tggtttggtt ggagaaaaaa
     7921 ctttaccaat gtataataaa gttgaagcaa caagatttat tgcagatgtg gtatatacta
     7981 ataagatgcc agcaggagca cttagagggt atggagcatc acaagggtgt tttgctgttg
     8041 aatcaactat aaatatgctt gctaataaat taaaaatgga cccaacagat ttaaggttaa
     8101 aaaatgtggt aacagaaggt gaaactacat ttgcatatgg taaaacttta aatagtgtcg
     8161 atttaataaa atgtataaat aaaggcaaag aacttattgg atggaatgaa aaatatcctt
     8221 gtaaacatct tgataatgga aaaattagaa gtgtgggtat ggcaattact atgcaaggtt
     8281 ctggtataca aggtcttgat aagtcttcag ctgaaataaa attgaatgac tgtggagatt
     8341 atactcttat gataggttct actgatatgg gtacaggaag tgatacgatt ttagctcaaa
     8401 tggctgctga ggttcttgat acaagcattg aaaatataac agttatatca gctgatactg
     8461 acttagttcc atatgaccca ggttcatatg cttcaagtac tacttatgta acaggtatgg
     8521 cagtagttaa ggcagctcaa gacttaagag aaaaaataat ttctgctgga gcaactaaac
     8581 taggtgttga gaaggatgaa gtagaatttg atggtataaa attaactagt aaaaaaggta
     8641 gcactttatt attcgatata gcagttgatt ctacaattgg tccagatact aatcaattaa
     8701 taggtcttgc ttctcatggt agcccagtat ctcctccacc ttttatagca ggttttgcag
     8761 aaactgaaat tgacacagaa actggaaaag ttgatttagt aaattatgta gctgtagtag
     8821 attgtggaac ggttataaat aaaaatctag ctaagataca agttgaaggt ggaattgttc
     8881 aagggattgg aatgtcatta tttgaagaag ttagatacac agaaaaagga agcatggaca
     8941 caaatacatt tatgcaatac aagattccag ctagatgtga tattggaaat gttaaagtag
     9001 attttgttga aacttatgaa ccaactgggc catttggtgc taaatctgtt ggagaagttg
     9061 ttataaatac acctttacca gcaatacaag aagctgtgtt taatggaatt ggtgtaagaa
     9121 taaatacact ccctattaca cctgaaaaag tttttatgga aatgaataaa taattattta
     9181 tgaaaattta gactagtttt acatgttaaa caaccagtta tgtcatacct gttgaaagat
     9241 atttaataag tagttattat aatattatga attttactta taaaatacaa aattaaataa
     9301 ggggatgatg ttttggctgg taatctaaat aatatgagag cagtaaataa ttttagagga
     9361 gataagaaca ttttagaatg tttagtcagc tttgagggtc gttcaataag tcagagaaaa
     9421 gtaagggtat tttttaaaga aaaacaaaat caaatagaaa ttgattttgc agaagaggaa
     9481 atttctaaat tggttgaaaa tgttgtttta aatacatcat atcaagaaat gttatatgat
     9541 gaaatagaga aacaactgga aattgattgt ataggtactt ggatgatatt atctaaatta
     9601 aaagatggta gtagagttca ctgattttaa aataaattat tagagcctaa ttttaaggct
     9661 cttttttgct gtttttaatt aacaatagaa aatttatctt tagtttgtat ataattttaa
     9721 tatcatatat tctatatata taaaattgaa tttgtatgta ttatattgta taatataaaa
     9781 tgaattggga aaatagttcg ctaaaataat ttgtttttat aaaattactg tttaagttta
     9841 tagattttgt ataaaataaa gttatgttat taattttttt aaaaataaaa cttttactag
     9901 gagacagata tgagaaaaaa tagtattaat atagcactaa ctgcagcaat tacagcaatc
     9961 tatgcagttt taacattatc attgggattt ataagttatg gaccaataca atttagggta
    10021 gcagagatta tgatgctttt ggcattttta gataaaggat atatagtagg tcttacatta
    10081 ggttgttttt tagcaaatgt aataggacca tatggtgtac ctgatataat ttttggaaca
    10141 ttagccacat ttataagtgc aagtatgatt tatattacta gaaagttagg taaacagaat
    10201 aaaagtactt tagttatagc ttctttatgg ccaactgtta taaattcgat tgtggttggt
    10261 ctaatgttaa atgtattctt tgggcttcca ttaattctat ctatgataca ggtaggtttt
    10321 ggagaatttg tagtagtaac aattgtagga gtaccatttt tctgtttttt aatgaaaaaa
    10381 tataaaaatg tcattaatat taaattttaa atagtgtata gtaatttaat taattgagag
    10441 tattattagt gaaaagaggt gtttatttgt cgaaggtaaa aagattgact gatgtttgga
    10501 tatatattaa tgataagcaa aagtttacaa taaaagagtt gtcagaggaa tttaatcttt
    10561 cagccaaaac aatacaacga tatttaaaag aattaaataa aatgggactt ccaattcaag
    10621 cagaacaagg tagaaatgga ggttataggg tgctaaataa tagctatata cctcctgtta
    10681 tatttacaga aaaagaagtt atgattatac tatttgcact taagtcactt cagatttata
    10741 attataagtt aatggaaata gaaattaatt ctataatgag aaaagtaagt tatgaacgta
    10801 aaagcagtat aaaagtaggt atagaaaata ttaaaagata cattggcttt gtagctaatg
    10861 atactaatga tggagatttt aagagtagta atgtaactga gttttttaga gcttcttcag
    10921 aatctctaat tttaaatatt aagtataagt gtggtaatca gattttggaa aaaaacgtat
    10981 gccctatagg tatatattta aataagggag catggttttc accagcatat gattgtaaaa
    11041 gtgataaaat aatattagtt catatagata atataattga tataaaaaga acaggagaaa
    11101 gtaaaaaaat acatataaac ttggaagaat ggttacatga agtatataac aatttatatg
    11161 gaaatagttc aactactaaa aaatttggtg aaattattta tctaaatgta ttattaacta
    11221 aagaaggtgt ttctaaaata aaggacagtt cttggaattt agatatggag ctaaatgaag
    11281 atggtagtgg aataattagt acattcataa aggttgaaga tatagattgt tatgcttcga
    11341 ttttattttt tattggaaat aatgctaagg ttatagagcc aaaatttttg aataagctac
    11401 tttatgataa ggccaatgag ttaagatatt tttatgaaga aaatatggta taatatttag
    11461 aaatgttgaa attaaataat tattaaggag atatacgtta taatggaaaa ttttaataag
    11521 aatgctaaaa taattggtat tggagctgaa ggtattaata taataaatga agtagaagaa
    11581 aaaataaaag caaatatgga tatagaaaaa ataaacataa atcaagagat tgaaaaagaa
    11641 tatgttagaa gtcttttaga tggtgtagat atattatttt tgatatattc atctgaagat
    11701 aaacatatta gagatattat aaaagctgtt tcatatatgt caaacgaaag aagagttcta
    11761 tctataggaa tggattgttc agagaaagaa aataaagaag atttagagct tgggagagag
    11821 tttaaaatca ataatgatag tatatttaaa tttgttgatt taatgaatat catggtagag
    11881 tctatttctg attcttgcat gataaatata gatattactg atttaaaaga agctattgta
    11941 ggagataaag gaattaagta ttcttttgaa gagtttgaag atacaaagtc atatagcgag
    12001 atagctgaca ttttatttga caggatggaa tatattggtg aggaatttat aagtaagaaa
    12061 ggaattgtat ttgttgaagg aagtccagaa ttttctataa tggaattgaa tgatttaata
    12121 agtaatattc agagcaaggt agaagaaagc tatgaagtaa tattttcttt atatataaag
    12181 gaaaatttaa atggaaatat tagagtaggc ttattgtata attagtatat tttagatatt
    12241 aaggagagtg tatatgtctg aaaatataag aggtacagag tattggttaa tggaagaatt
    12301 tttttatcag gaaacagaca caccacagat ttttagagaa gaaagcataa ttgcacttgg
    12361 atttgatata aaaataaatt taaaaaaagt attcagtatg gggaaaaaat cagaaaagta
    12421 tattaatgag aattgtaatg ataaagattt tataaaaaag tttatgtcga ttaaaaaagg
    12481 agattatttt ttatataaag catatcaaaa tatagaagaa aaagttaaat gtaatgtgtc
    12541 tattggcata gttgaagaag attttgaaaa tggatatgaa ttaagtcata ttttagggca
    12601 tacactacca gtaaaatgga tgcataattt tgatacagtt tcaaattgta aagtatatac
    12661 aagtgaattt tataaattaa ataataatga tattagctca tatttagaaa ttattaagtc
    12721 acaagataag aaattattga gaaaaagaat agaagaaagt gaaaataata aatggtattt
    12781 aaaaaactat tattataaaa atgtagataa gtgtgtagat gatgaagaat tatttattca
    12841 gcccattaat ctagaacaat tagaaagtct aaaaaatata aagacagatg attatatttt
    12901 attgtataat agcgagaaag taatttgtga aaatgaccat acaatgaaga gtctatttgg
    12961 cgtatgtcaa gttatggaag ggttcgataa agagaaagta agtgctttgg agggtggata
    13021 tttttttcct gttagatgga tagagtatga aaaggagaat atagacggaa atgcatgggt
    13081 tatatttgaa aataatagta tagttgaaaa gtatgtaaaa agatttacta ataaaatgcc
    13141 attaaatact atactttatg gaccaccagg aactggtaaa acatacaata caaaacagat
    13201 ggtttacaat ctagtaagtg aaaccagtct aagaaacaga tactatacag gttacttaga
    13261 ttataaaaac aatgtagaat tttgtacatt tcatcaatca tatggatatg aggaatttat
    13321 agagggtctt aagtctgatg gtgaaggcaa ttttaagcca gaagatggga tattaaagga
    13381 tatatctata gaagcatgtt ataatgcatt gaaatttgaa aaaaagatga gctttgagtt
    13441 ggaaaaagat agcttgacgt tagaaactat aaaagacagg aaaaagaaat tagtattaga
    13501 aaatatagaa tatagtgaga gttttaattt tgaagaatca gaaaactatg ttttaataat
    13561 agatgaaatt aatagaggga atgtatctaa aatatttggt gagctaataa cattattgga
    13621 agatgataaa agacttacta aatcaaatca aactattgtc aaactaccat attcaaagga
    13681 aaaattttct ctacctccaa atttgtatat agttggaact atgaacacat cagataaatc
    13741 aatagcactt ttagatatag ctttaagaag aaggtttaat tttgaagaaa ttatgccaga
    13801 ttatgattta ttgtctattg tagatggtat agatattaga aaaatgctat atacaatcaa
    13861 taaaaggata gagtttttat atgatagaga ccatgttatt ggacaagctt atctacttgc
    13921 tgtagaaaat attaatgata tagtagtagt atttagaagt aaaataatac cacttcttca
    13981 agagtatttt tattatgatt cagaaaaagt aggtttgatt ttaggaggaa ttggaagaaa
    14041 tgaaaaagat aaatacattg tttataaaga aaaacttata gcttctgagt tatttaaaaa
    14101 taataatgcc tcaataatgg agagtaaagt taactattgt ataaaacaaa acatatctag
    14161 tgaggagcta aaaaatatat atgagtaaaa atatatatgt taaggaaacc tatgaatgga
    14221 taaggatagg taatggtgaa aatgagctta cagaaattga atatgaaaaa ttgcttaagt
    14281 atctagagaa caataatgat gtattaaaaa gtaatataat agatattaaa tacaaaaaac
    14341 ttagatttat aaactatgta ggcatcatat gttttgaaaa tgtaatactt gaaatacttc
    14401 ctaaattgtc attaagtgat aatcttgtaa aagatagaga gatactatta cagatgcttt
    14461 ctatatgtaa taaaattcca attacaatga atgaaaaaat aagattgagt ttgaaaaatt
    14521 ataatctctt aaattttttt gttatgtatt tcattgagag tatgcaaaca cagatgaaaa
    14581 aaggtattta ttttgaatat ataaataaaa tagagaactt aaatgttatg aggggtaaaa
    14641 tattattaag tacatatgca aaagaaaaag gcatttctcc aatgaaaata agatgtgaat
    14701 atgatgagta tagtgaaaat aatttcttga atcaagttct taaaaaagca tgtatatcta
    14761 ttttatgtag aataaatgac aattcaattc aaggcaagat taaaaaaatt ttaagctatt
    14821 ttcaaaatgt agatttaatt tatatagata gaaaaaaact attggattat aaattttata
    14881 aaaacaatga tagatttaaa gattgttatt tactagctag acttctactt ttaaatttgt
    14941 ctatggataa tagtcaaaat aatcaagagg cattttctat attatttgaa ataaatactt
    15001 tgtatgaaga atatatagga attttaatca aaagtatatg ggacaattca tttagagaaa
    15061 catatataca agataagagt aaatttcttt tgaaaaatga acaaacaggt aagaaaaact
    15121 ttaatttaag accggatata gttttatatg acttaaaaaa tgaatatgaa attataattg
    15181 acactaaatg gaaagctatt gaagtagatt caaatgtttt ttataggtca agtgatatat
    15241 accagatgta tgcatatata acagcatatg aaaatgcaaa aagatgtata ttattatatc
    15301 cttgtattca aaaagataag aattatagtt cctggaaatt atcagaatca tttaagggta
    15361 agtttataga agcaaaaact gtgaggttag atgatataaa aaa
//
LOCUS       DAGSRM010000093        15221 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.093, whole genome shotgun sequence.
ACCESSION   DAGSRM010000093 DAGSRM010000000
VERSION     DAGSRM010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15221
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.093"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            145..480
                     /locus_tag="MJE33_002976"
     CDS             145..480
                     /locus_tag="MJE33_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY9648898.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQEDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNVVESTYFLSESGLRLLPVMEKMIMWS
                     NKNLNCDKY"
     gene            617..1894
                     /locus_tag="MJE33_002977"
     CDS             617..1894
                     /locus_tag="MJE33_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HBY9648899.1"
                     /translation="MQNVNLFLVFIEGIVSFFSPCILPILPIYLSILSNSSVENLKEG
                     KTSFIGSSLFKNTIFFALGISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGL
                     FYMGIIKSSILNREKRFNVKFKEMKAITAFILGFTFSFGWTPCIGPILASVLVMVSSS
                     SNHLSANLLIAVYTIGFILPFIITAMFYSKLFKTIDKIKSNMEIIKKIGGIILIVSGI
                     LMMVNGFGSISKHFNTSQNSKIESKQEENKRENSTDKEENSDGNDSQKDSNNDNNDKG
                     SNDEDRIKSIDFTLTDQYGKTHKLSDYEGKVVFLNFWATWCPPCKEEMPYIEQLYKDY
                     NKNNDDVVILGVASPNLGREGSREHVVNFLKEQGYTFPVVLDEDGALAYQYGINAFPT
                     TFIIDKEGYVTQYIPGAMDKATMASFIENQRNK"
     gene            2015..3403
                     /locus_tag="MJE33_002978"
     CDS             2015..3403
                     /locus_tag="MJE33_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBY9648900.1"
                     /translation="MKKTFDAIIIGFGKGGKTLAGDLANRGLKVALIEKSNKMYGGTC
                     VNVACIPTKSLENSANSVKTKNINSWDEVQAEYEKAIDKKETLITKLREANYNKLNSN
                     ENVTIFTGMGTFIDEKTVQVKTENEIYELVADNIFINTGSRPFIPNIKGIENKNIVYD
                     SESLMNLRVLPKKMTIIGAGFIGLEFAGIYSSFGAEVTILNSNNGILPNEDVEDSEEI
                     IKLLAKRNVKIVNNANIKEIKEVSELAIVEYEVDGKSKELTSNMILVATGRKANTEGL
                     GLENAGIELNERGFIKVSETLKTNKEHIWAIGDINGGPQFTYISLDDYRIVINQLFGD
                     KTRKTNDRKNIPNSIFISPAFSRVGLNVKQAKEKGYEVLVAKMPVEAIPRAKQIGKAD
                     GFIKIVIDKKSNKILGASMICENSSEIIHLIQLAVDLEVEYTYLRDRVYAHPTMTEAL
                     NDILSPNMIKEV"
     gene            3774..4487
                     /locus_tag="MJE33_002979"
     CDS             3774..4487
                     /locus_tag="MJE33_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017208807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HBY9648901.1"
                     /translation="MNKISKRGFDDSDKRWFSSKELTRLIKAKEEIEWLLNRDYKIDP
                     VVTFVGDRYQFSIRQRDALKRAVCTDEKNIIRQSKRLSLDKIKEDTVNIDGFNLIISI
                     EVGLSGGTLVIGSDGNIRDLAGLRGTYKIIDKTEEALNLIGKFFNKYNAQNLKFYLDA
                     PVSNSGNLKYRILEHAKTWGIETEVELVKNADVVLEKLDRVVSSDAVIVDKCISYFNV
                     ARGIIEEYIKECNIINLNK"
     gene            4605..5474
                     /locus_tag="MJE33_002980"
     CDS             4605..5474
                     /locus_tag="MJE33_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBY9648902.1"
                     /translation="MRGWICVKLTKLNIKKYRAPIYKYALANVNLRSAKSTNSSIITV
                     IPQGAKMEVLDEEDDWIKVMYNSQEGYVYKDLVSVSEYAWSNLNLREDKSTTSNIITV
                     IPEKSRVEVLQVDGDWSKVVYDDKIGYVFNYFLSIDGNKPNELDYKYFYTDMIKFVNE
                     NNIKSTSDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPETPTITGIFYISGRK
                     PSFGTDEYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWI
                     YDNIPDTTTVIIH"
     gene            complement(5629..6774)
                     /locus_tag="MJE33_002981"
     CDS             complement(5629..6774)
                     /locus_tag="MJE33_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBY9648903.1"
                     /translation="MSIYYVPPINLLGKGCLNEAKDSIKSLGTKKAFIVSDKFLVSNG
                     TVKKVTDILSQIDIDFVVYDEVKQNPTVTNVNKGLKILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIIAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDTNSLATMTVNDPELMISKPSNLTAATGMDALTHAIEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSAPAKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENSMKDACAGANPVFFDKDKIIELFMKIS"
     gene            complement(7107..7883)
                     /locus_tag="MJE33_002982"
     CDS             complement(7107..7883)
                     /locus_tag="MJE33_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBY9648904.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADVINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPD
                     GLKGFTEKSITDFDVLYDQLLEVQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
     gene            8465..9439
                     /locus_tag="MJE33_002983"
     CDS             8465..9439
                     /locus_tag="MJE33_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBY9648905.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTITA"
     gene            9537..9842
                     /locus_tag="MJE33_002984"
     CDS             9537..9842
                     /locus_tag="MJE33_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBY9648906.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGSEISFKLKDKTVKTITALDDI
                     TIYHKLAIKDMAKGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            9998..11158
                     /locus_tag="MJE33_002985"
     CDS             9998..11158
                     /locus_tag="MJE33_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBY9648907.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQIDKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNMD
                     FDASPVIYGPQTMEELTDDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            11208..12110
                     /locus_tag="MJE33_002986"
     CDS             11208..12110
                     /locus_tag="MJE33_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBY9648908.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNVSSEDIIKLKTIINESTFYNYDVFKRERCTVHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDDSFEEPQKTVYFTKRVTKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSAVPFPIK
                     LKVCSRVNGEERQSSNTGLFISDVSDIVSELSKGMTLEAGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            12190..13083
                     /locus_tag="MJE33_002987"
     CDS             12190..13083
                     /locus_tag="MJE33_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HBY9648909.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDKEGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVTSHVNKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAEIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKIEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMEKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNELNIC"
     gene            13789..14262
                     /locus_tag="MJE33_002988"
                     /pseudo
     CDS             13789..14262
                     /locus_tag="MJE33_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014521021.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14252..15097
                     /locus_tag="MJE33_002989"
     CDS             14252..15097
                     /locus_tag="MJE33_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003485307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9648910.1"
                     /translation="MYSKIAIKNVRKSFKDYSIYFLTLTLVICIFYSFNSMESQKALM
                     EMSTSKKPDLEMFKNMISYISVFVSAILCTLILYINNLLIKKRNKKLGIYMTLGMGKI
                     EMSKILVFETIIVGIVSLVSGLILGLLVSQGLLILLLKLFEFNMSEYSFVISIPAIIK
                     TIVYFGIIFLLVMIFNIYLISKYKMIDLLIVERKTEDIKFKNPFVYMITFVSCFIFLF
                     TVYNTTNHIIINLIDDKQLNSKSKGVEINEFTYQDWQQFCDIKYYGKKGLISMTLKEY
                     IEKYK"
BASE COUNT         5949 a         1643 c         2560 g         5069 t
ORIGIN      
        1 atatataaat ctaaaatagt ttgtaatagg agtatatata ataaaaattt taattaaata
       61 aagctaggta tatttataat attaataaat atattgctat aaaagtttgt ttaataaatt
      121 aaataaagaa aagaggagaa taaaatgtac aacatagatg atatgatata taattgtcca
      181 gtagaagctt tatcaaatat tttaggtaaa aaatgggtgg ctataattat atggaaaata
      241 caagaagata aaaaacgatt tggagagcta caaagagcta taccagactg tagtaagaaa
      301 atgttagttc aacaactaga ttttttaatt gaacagggga ttatagtcaa ccaaaaaaag
      361 ttggttaata atgttgtaga gtcaacatat tttttaagtg aatcaggatt acgattgctt
      421 cctgtcatgg aaaaaatgat tatgtggagc aataaaaatt taaattgtga taaatactaa
      481 aatttactct gtaagtaatg aaaatttaat taagctatga gtagaaaaat gtattaaaat
      541 tacataattt tttagttaga ttttaattta gaaatgggat tgtaatattg gaaataaaat
      601 aaggaggaat tttaatttgc aaaatgtaaa tttattttta gtatttatag aaggtatagt
      661 atcattcttt tcaccatgta tactgccaat tttgcctatt tatttgagta tattgtcaaa
      721 tagtagtgta gaaaatttaa aagaaggaaa gacgagtttt ataggaagtt ctttatttaa
      781 aaatactata ttttttgctc ttggaatctc aacaacattc tttatattgg gttcatcagt
      841 aaaagtattg agtatgtttt ttaatgaaaa taaagattta ataatgttta ttggtgggat
      901 tataattatt atcatgggat tattttacat gggaataatt aaatcttcaa tattgaatag
      961 agaaaaaaga tttaatgtta agtttaaaga aatgaaagca ataacagctt ttatattagg
     1021 ttttacgttt agttttggat ggacaccttg tattggtcca atattggcat ctgtattagt
     1081 catggtttca agttcaagta atcatcttag tgccaatctt ttaatagcag tatatactat
     1141 tggttttatt ttgccattca taattacagc tatgttttac agtaaactat ttaaaactat
     1201 agataagata aaatctaata tggaaattat taaaaaaata ggtgggatta tacttatagt
     1261 atcaggaata ttaatgatgg taaatggatt tggaagtata agcaaacact ttaatacgtc
     1321 acaaaatagt aaaattgaaa gcaagcaaga agaaaataag cgagaaaatt ctactgataa
     1381 agaagaaaat tctgatggta atgatagtca aaaagactct aataatgaca ataatgataa
     1441 agggtctaat gatgaagata gaataaagtc tattgatttt actttaacag accagtatgg
     1501 taaaacacat aaattaagtg attatgaagg caaagttgta ttcttaaatt tctgggctac
     1561 atggtgtcct ccatgtaaag aagaaatgcc ttatatagaa caactgtata aagactataa
     1621 taaaaataat gatgatgtag taattttagg agtagcttca cctaatttag gtagagaagg
     1681 ttcaagggaa catgtagtta acttcttaaa agaacaaggt tatacattcc ctgttgtact
     1741 tgatgaagat ggagcattag catatcaata tggaattaat gcttttccaa ctacatttat
     1801 aattgacaag gaaggatatg taactcagta tattccagga gctatggata aggcaactat
     1861 ggcatcattt attgaaaatc agagaaataa ataaaagtat ttaagtataa gtttataaat
     1921 caatttaagg ttacaaaaaa gtgcctaatt gtaccaccta aataatcttg ttatagtaaa
     1981 agtataaaca atacaagaaa gggttgaatt ttatatgaaa aaaacatttg atgctataat
     2041 aataggattt ggaaaaggtg gaaaaacatt agcaggggat ttggcaaaca ggggattaaa
     2101 agtagcttta atagaaaagt caaacaagat gtatggtgga acatgtgtaa atgtggcatg
     2161 tataccaaca aaaagcttag aaaattcagc aaacagcgta aaaacaaaga atataaattc
     2221 ttgggatgaa gtacaagctg aatatgagaa agctatagat aaaaaagaaa ctttaattac
     2281 aaaactcaga gaagctaact ataataaatt aaattcaaat gagaatgtaa ctatattcac
     2341 aggaatggga actttcattg atgaaaaaac agttcaagtc aaaactgaaa atgaaattta
     2401 tgagttagta gcagataata tatttattaa tactggttca agaccattta tacctaatat
     2461 taagggaatt gaaaataaga atatagtata tgatagtgag tctcttatga atctaagagt
     2521 tttacctaaa aaaatgacaa ttataggagc tggatttata ggtttagaat ttgctggtat
     2581 ttatagttca tttggagcag aagttacaat attaaactct aataatggca ttttaccaaa
     2641 tgaagatgtt gaggattcag aagaaataat aaaattactt gcaaagagaa atgtaaaaat
     2701 tgttaataat gcaaatataa aagagattaa agaagtttct gaattagcaa tagtagaata
     2761 tgaagtagat ggtaaatcta aagaattaac aagtaatatg atacttgtag ctacaggaag
     2821 aaaagcaaac actgaagggc tagggctaga aaatgcaggt atagaattaa atgaaagagg
     2881 atttattaaa gtctcagaaa ctcttaagac taataaagag catatatggg ctataggaga
     2941 tataaatgga ggtccacaat ttacttatat ttcattggat gattatcgca tagttattaa
     3001 tcagttattt ggagataaga ctagaaaaac taatgataga aagaatatcc caaattctat
     3061 atttataagt ccagcatttt caagagttgg tcttaatgtt aagcaagcta aagaaaaggg
     3121 atatgaagta ttagtggcaa agatgccagt agaagcaatt ccaagagcta aacagattgg
     3181 caaggcagat gggtttataa aaatagtgat agataaaaaa tccaataaga tacttggagc
     3241 tagtatgata tgtgaaaatt caagtgagat tatccatctt attcaattag ctgtagattt
     3301 agaagtagag tatacatatt taagagaccg tgtttatgca catccaacta tgacagaagc
     3361 tcttaatgat attctatctc ctaatatgat aaaagaagta taacataagt ttaatatata
     3421 aacctaaaat tatcattata agtagatttt agaaagagac tatctcaaaa aatagagata
     3481 agtctctttt ttactgttta aataataaaa atatatttga ttttataaaa gaagattatt
     3541 ttatgagcta aaaagtttat ttagaaacac tacctattta tgttataaag ttataaatat
     3601 aatatagaat ctattattct gtattattaa gtggggttaa gcgttataat taattataaa
     3661 tattgaataa tctaaatgga taagataatg gtattaaaaa ttatcttaaa aggtgaaata
     3721 atctgtattt aatgtagtac agttatatct ttaaagcaag gtggtgaaat gatataaata
     3781 agatatctaa aagagggttt gatgatagtg ataaaagatg gttttcaagt aaagaattaa
     3841 caagacttat aaaggctaaa gaagaaatag aatggttatt aaatagggat tacaaaatag
     3901 acccagtcgt tacttttgtt ggtgatagat atcagttttc tataagacaa agagatgcat
     3961 taaaaagagc agtctgtact gatgaaaaaa atattataag acaatcaaag aggctttctc
     4021 tagataaaat caaagaagat accgtaaata tagatggctt taatttgata atatccatag
     4081 aggtaggatt atctggtggt actctagtaa taggaagtga tggcaatata agagatttag
     4141 caggtcttag gggaacctat aagattatag ataaaacaga agaagctttg aatctgatag
     4201 gtaaattttt taataaatac aatgcacaga atcttaaatt ttatttagat gctccagttt
     4261 ctaattcagg aaatttaaag tataggatat tagagcatgc caaaacttgg ggaattgaaa
     4321 ctgaagtaga attagtaaaa aatgcagatg ttgtattgga aaaattagat agagtagtta
     4381 gtagtgatgc agtcatagtt gacaagtgta taagttattt taatgtggct agaggtataa
     4441 ttgaagagta tattaaagaa tgtaatatca taaatttaaa taaatagata aatataggtg
     4501 agaatatata agtatatgta gcaatattct tagtaataca agtaaagttt gaaaatagga
     4561 gaacgaatac atatcgaatt tcataaaatg ttgtaaaaag acatatgaga ggatggatat
     4621 gtgtgaaatt aactaaacta aatattaaaa aatatagagc acctatatat aaatatgctt
     4681 tagcaaatgt aaatttgagg tctgctaaat ctactaattc aagtatcata acagtaatac
     4741 ctcagggagc caaaatggaa gtattggatg aagaagatga ctggattaag gtgatgtaca
     4801 attctcaaga aggatatgtg tataaggatt tggtatcagt aagcgaatat gcttggagta
     4861 acttaaactt aagagaagat aaatctacaa cttccaatat aattacagta attcctgaaa
     4921 aatctagggt tgaagtcctt caagtagatg gagattggag taaggttgta tatgatgata
     4981 aaataggata tgtttttaat tattttttat caattgatgg gaataaacca aatgaattag
     5041 attataagta tttttatact gacatgatta aatttgttaa cgaaaataat attaaaagta
     5101 ctagcgatta tctaattgta acagacctta gaaataaata tacttatatt tttaagaaag
     5161 ataatggagg ttggggacaa ctttacaagt ggcaatgtac tataggcaag cctgagactc
     5221 caactataac aggtatattt tatataagtg gaagaaaacc ttcttttgga acggatgagt
     5281 attcagttaa gtatgctact agaattaaag gtggatatta ctatcattct gtactatatg
     5341 attcaacagg aagttatata atagatggca gacttggtga agctcttagt catggatgta
     5401 taagattaag tacggaaaat gcaaagtgga tatatgataa tataccagat acaacaacag
     5461 ttataattca ttaagaaaaa ttttgagttt tttattggga taatgattct taaattaata
     5521 ggattatata ttaacttctc ataaagagta ctatttaaaa agtgtaataa gagagtgcta
     5581 ttaacaaaac aaagatattt tgaggcactc tcttataaat atacataatt aagatatttt
     5641 cataaacaat tctataatct tatctttatc aaagaaaaca ggattagcac cagcacaagc
     5701 atctttcata gagttttctg caagcaattc aaaattagga ttatcaacac ctacatcttt
     5761 taaagatttt gggattccaa cctcttcaga aagagctttt atcttatcta ttacaaagtc
     5821 aacacactct ttatctgatt tattatttac atccattcca ataacttcag ctattggtcg
     5881 gaatttggca ggggctgatt tagcattttc ctcctcaact attggaagta gcatggcatt
     5941 acatactcca tgaggtaaat catataaacc tcctaattga tgagccatag catgtacatt
     6001 tccaagacct gcattactaa aggcaattcc atttaaaaaa caagcataac acatttgttc
     6061 tctagcttct atgtcattac catttttaac agctcttgga aggtatttaa atatctgctt
     6121 tatagcatat aaggcagttg aatctgtaac atcataagca ccattagcta ctacagcttc
     6181 tatagcatga gttagtgcat ccataccagt tgctgctgtt aaattagatg gtttgctaat
     6241 cattaattct gggtcattta ctgtcattgt ggctagtgaa ttggtatcaa ccattatcat
     6301 ctttacatgt ctgtcttcat cagttataac atagtttatt gtaacttcag cagatgtacc
     6361 agcagtagta gttatagcta tgataggaag acatttttta gaagttttgt tgataccttc
     6421 atagtctcta atatctcctc cattagtggc taatatagag atagctttac cacagtcttg
     6481 tggagaacca cctcctatag ttataacgaa gtcgcaattt tctgatttta atattttaag
     6541 tcctttattc acattagtta cagttggatt ttgtttaact tcatcataaa caacaaagtc
     6601 tatatctatc tgagataaaa tatctgttac tttttttaca gtaccatttg aaactagaaa
     6661 tttatcacta acaataaatg cttttttagt tcctaatgat ttaattgaat ctttagcttc
     6721 attaagacat cctttcccaa gtaaatttat tggtggtaca taataaatgc tcataactaa
     6781 aatctccttt tattttcatt gatttttatg taaaaatata tatctaacaa ttttcactaa
     6841 tttactcata atatatcgta tcttagattg agatagggtt gctggttctt atataagaac
     6901 ttaattcgta attacgatta tgattagaat ttaacatagt atttttatca tgtcaataaa
     6961 aatataaaaa atattataaa aaaaacaaag ttactgtagt taaatcagca actttgtttt
     7021 tatagtataa atcaaataaa tacattagtt taaaacaaaa tttttaatta ttatgttttg
     7081 atatcttcca tatacagaat attaatctaa tttattacaa tgtcttcatt acgttcttcc
     7141 atcattgatt gaatattttt ctgagcattt aagagtaatt tttttatcag ttctattttt
     7201 tcgtcattag ctctaaataa aggcagtgtt acactaacag cagctcttat acatccattt
     7261 tttcttattg gtacggcaag acattttagg tgttccattg attcctcaca ctctgaagct
     7321 atttgtgttc tttgaacttc aagaagctgg tcgtataaaa catcaaaatc tgtaattgat
     7381 ttttctgtaa aaccttttag accatctgga tataagctta ttagtttttc cttgtcaaaa
     7441 tcacataaaa gagcttttcc aagacctgta caattagcag gtattttttt tccaacataa
     7501 gaaataagtc taatcggttc tggagaatct acttttccaa tataaagaac ttcattattg
     7561 tcaagtatac caagctgaca agtttcagaa caggaattaa ctatattagt catttcgtca
     7621 attatatagt taaaaatagt tcttttttca aaaaatgaag accctaatgt gtaagaacgt
     7681 atccctatag aatatttgga tgtgttttta tcaatagtaa tgtattttct agcattaagt
     7741 gtatgaacta taggaaagat gctacttttt ggagcattga taacatctgc tatttctgta
     7801 agagtatatc cattttcgga ttctgctaat gcttctaaaa tatctaatat cctagctgtt
     7861 gggcgatgat tatctgaagt cataaatttc cccctctttt tattaattta aagtattaga
     7921 atatgttgat tttagcacac tatacgatat tttacaatgt agaaaataaa tatgtaaatt
     7981 tatatataaa taagctcttg atttagatac agatagatat ttttttagac tgtacattaa
     8041 ggaataacta atttccaaaa tatacctaaa gaaagtaagt gatatttagt tatataattt
     8101 aagtaaatat atgcaatagt tatttttata tgtaaatatc aaaatattat gaacttatat
     8161 tttatctatt ttttacactg tataaaaact ttatagtttt gaatactata taattttaaa
     8221 tacgctgtat ttttgcatat aaggaagtca tttgataata tacaaggtat tatcagaaag
     8281 tttgaaaaaa ttttgacaaa aatttatttt ttttattgac tcataaatag gaaaatgcta
     8341 tcattaattc aaagattcgt aattaaaaac ttagttctta aatacgaacc gaaacggccg
     8401 ttagaattaa agctaaaaac ttaataagag taattaaata tcatgtttta ggaggattaa
     8461 ctttatgata atggatttta ttaaaaaaat tcctgcaggt atgatgatag tgccaatgtt
     8521 tatagcagct ttcattaata ccttttgccc agatattgta caaataggtt cgtttacgac
     8581 agcagtattt tcaagtgttg ggtctgcaac tataattggg gttcaactgg tatgcttggg
     8641 aactcagctt caatttagag aaatgccaaa agttttaaag agaggtggta tacttcttgg
     8701 ttctaaattt gctataggtg ctttgatagg tatacttata ggaaagctat ttggaatgga
     8761 tggtgtcctt ggtctgacaa cattggctgt tataagtgca gttacaaata gtaatggtag
     8821 tatatatctt tcattgatga cttcatatgg agatgaaaca gattgtgcaa ctatgagttt
     8881 acttacttta aatgatggac cattttttac attaatagct cttgggactt caggtcttgc
     8941 aaatgtacca ttgttgtcat tattggctgc aatagtgcca atacttgttg gtatgataat
     9001 aggaaatttt gacaaaaaaa tgaaagagtt tttagagcca gggtgtaatt tactcgtacc
     9061 ttttgtaggg tttgcacttg gtagtagcat aaatcttgca agtatactaa aaggaggttt
     9121 atcaggagta ttacttggat taatatctgt atttgtagga ggtatattta tagtattctg
     9181 tgataaattt ataggaaaac gtccaggata tgcaggatgg gctgtagcta caactgctgg
     9241 aaatgcaatt gctgtacctg ctgctgttgc attagttgac ccatcatggc aaccatatgt
     9301 tgcaacagct acaacacaag tggcagccgc agtagttgtc actgcaatat tagtacctct
     9361 tataactagt tggtgggcta agaagtatgg atgtcctaag ttcccaaaac aaaatgagga
     9421 tggtacaata acagcttaat ttatattaca gtgagttgtt ggtaaaataa taaattcttt
     9481 aattagattt taggatatta ataaaattat attatttata aaggagagca agaataatga
     9541 taaatgcagt gataattgat gaaaaagata atgttgcagt agctattgaa gcaatagcta
     9601 aaggtagtga aattagtttt aaattaaaag acaaaacagt aaaaacgatt acagcactag
     9661 atgatataac tatttatcat aaattggcta taaaagatat ggccaaaggt gataaagtta
     9721 ctaaatatgg tgaacatata ggagaagcaa atcaggaaat aaaaagtggc caacatgtac
     9781 atattcataa tgttagaagt gttagagagg atttggataa acaagttatg actaatatat
     9841 aaaataatat ttatagagtt ataaaagttg tattcattgc ttttagtgtc aaatagtttt
     9901 taaataggtt tttagctgtt tgattagtga gtgtaataaa aattataata tataaaaatt
     9961 atagaaagta tatagactat tttaggagga aatcagtatg aaattttatg gatataaaag
    10021 accagatgga agagttggta taagaaacca catattaata ttgcctacga gtgtgtgtgc
    10081 atcagataca actagaataa tagcatcaca agttcaaggg gcagttacct ttaataatca
    10141 gaatggatgt tcacaagtac attcagacca acaattaact atggatgtta tggctggtta
    10201 tgcagcaaat ccaaatgtat atggtgtaat agttgtatct ttgggatgcg aaaattgcca
    10261 aaatgatttg gtagttgatg caataaaaga gagaacaaat aagccaataa aaactcttgt
    10321 aatacaagaa gaacatggaa ctttaaaaac tatagaaaaa gctgttcgtt atgctagaga
    10381 aatggcacaa gaagcttctt tgcttagaaa agaagaattt ccaatatctg aacttatact
    10441 aggaactgaa tgtggtgggt ctgacccaac aagtggatta gcagcaaatc cattaatagg
    10501 tgaattaagt gataaacttg tcgatttagg agcaacatca attttaagtg aaactactga
    10561 atttataggt gcagagcata tactagctag gagagcagta aatgaagaag taaaagaaaa
    10621 aatattacat atagtacata gatatgaaaa ttcattaaaa ttggttggag aagaagttag
    10681 agaaggtaat ccatctcctg gaaatatagc tggtggttta acaagtttag aagaaaaatc
    10741 acttggttgt atacataaag gtggtcatag acaaataagt gaagtatatg attatgccaa
    10801 acaaattgat aagaaaggtc ttgttataat ggatacccct ggaaatgatg cttcatctgt
    10861 tgctggtatg gtagcaggag gagcacaaat agtggtattt agtacaggaa gaggaacacc
    10921 atcaggaaat ccaatagcac cagtaataaa aataactgga aataaaataa cttttgcaaa
    10981 tatggaagat aatatggatt ttgatgctag tccagttatc tatggtccac aaacaatgga
    11041 agaattaaca gatgatttgt taaatatggt tgtagatgta gcaaatggaa aacaatcaaa
    11101 agcagagtcc cttggatata ctgaaatggc aatagctaga gtatgcaact atgtataatc
    11161 tttaagtaag aaaataaata catagaattt taataaaggg tgagattatg aagtttgtaa
    11221 gttttactga aggagaaaat gaatgtaaaa gaactggagt tttttctaaa gatgaattat
    11281 ttattataga tgttaactct ttaaatctca gtagaaattt taaggattta aatgaattaa
    11341 ttcaaaatgt atctagtgaa gatattatta agttgaaaac tattataaat gaaagtacat
    11401 tctataatta tgatgttttt aaaagagaga gatgtacggt acatgttcct atagaaaaac
    11461 caatacatga tatcttatgt gtgggagtga actataaaga ccatcttgaa gaaactcaaa
    11521 ctcactttga tgatagtttt gaagaaccac aaaaaacggt ttattttaca aaacgtgtga
    11581 caaaggcaat aggtccagaa gatgagatag atggtcattt tgagataaat aatcaactag
    11641 actatgaagt tgaacttgga gtagtaatag gtaaaacagg tgttaatatt agaaaagaag
    11701 aggtagaaga ttatatcttt ggttacactg ttataaatga tatttctgct agaaagttgc
    11761 aaaaagaaca tgttcaatgg tttagaggaa aaggattaga tacatttacc tctttaggtc
    11821 catgtatttt acataaaagt gcagttcctt ttcctataaa acttaaagta tgtagtaggg
    11881 taaatggtga agaaagacaa agttctaata caggattatt catatcagac gtttcagaca
    11941 tagtttcaga actttcaaaa ggtatgacct tagaggctgg agacattata gctactggaa
    12001 caccttctgg agtagggatg ggattttcac ctcctcgata tatgagtagt ggagatgtag
    12061 ttgaatgtga gattgagagt attggaattt taaaaaatta cattaagtaa ctgttttaat
    12121 ataattaata ttaaagctaa atctgatgta ttaatgaaca tattaaaata aattttagga
    12181 gtttaaatta tgaaagcaga atttttgaca ccagttgtta caatatttga tgaagaagga
    12241 aatttagata aagaaggtaa taaaaaagtg tatgaccatc ttatagatgg aggtgtagat
    12301 ggactagtca taatgggaag tactggagag ttctttaata tgtctatgga tatgcaaaaa
    12361 gaacttatag atttagtaac tagtcatgta aataagagag taaaagtgtt tataggtaca
    12421 agtcgtatgt gtataaatga atctatagaa ctttcaaact atgcacataa tagtggagca
    12481 gatggtgtta tgataataag cccttattat ttctctcttg atgatgatag tattgagtta
    12541 ttttatagtg aaatagctaa gaatacagag gctgaaatat atttatacaa ttatccagat
    12601 agaactggtt atgatttaag tccagaactt acattaagac ttgcaagaaa acataaaaac
    12661 atagttggat ataaggatac agtaactttg atgggacata ccagagattt aataaatact
    12721 atgaaaatag agttcccaga atttaaggtt tatagtggtt atgatgaaaa ctttatacat
    12781 aatctaatgt ctgggggagc aggttgtata ggggggttat caaatttaat tccagaagtt
    12841 tgctccaaat gggctgaagc ttacaataat agagatatgg agaaggttat agaaattcaa
    12901 aaatatatta ataaagcaat ggatttttac ttaataacca atccatttat accagcaatg
    12961 aaaaaagcta tgaatatcag agggttacaa ataagtgatt atatgactgc tccatttgta
    13021 aaaccaaatg aaaaacaagt atgtgaaatt aaaaaattga tgaatgaatt aaatatatgt
    13081 taacttacta agtaagatat atgagcaaaa aaagagtgtt taaactgatt tttataatca
    13141 ttttaaacac tctttttata tatgttaaat caactttgaa aagtggtcaa caataaatta
    13201 caattatgtt aataaaaatt aaataatact aagatagcag attagtttaa aagttagcac
    13261 aaatattaaa gcaatagcct ttatctatta aaaataatct tggagtttta caaacatttt
    13321 tagataagta taagttaaca ataagagcca catataaact actacaaatc atgtaataat
    13381 taatctaata aatgataaac acttagactt caagcttaaa agtataaaga taaatgaatt
    13441 tacatatcaa gattggcaat aattttgtga tacaaagcac tatagtaaga atgatttaat
    13501 accaatgatt ttgaaagaac atatagaaaa atacaattag aataaaataa attttagagg
    13561 gaaggcattg ccttccattt tttgtgtttt tctagaattg ggtaaatttt acgattctag
    13621 taaagcacaa aagaaaatct gttggtcttg aacattttat attagtaatt ataggtagca
    13681 atatacaatt actacaaaag tgaaatatta atgaaatgta aatcaattta aaaaaattaa
    13741 attaaaaatt acaatagata tataagaaat ttaaaggaga aataaggcat gaaaaatgta
    13801 ttaagtgttg aaaaatggaa aagtattatg aaaacaaaga taatattaca aaagcaatag
    13861 ataataatga tattacaaga ttaaaattca aaaacttaga taaatttaga caaaatgaat
    13921 taggatttat attccaaaat tttaatcttt tatatacttt aacagcttat gaaaatatag
    13981 cattgacatt aacaattaaa gggaaaacaa tataaatttg attcaaaagc atcaagatta
    14041 ttacttgaga gttttgaaag tttaaatcaa gatttaaaaa caacaatttt aatgctaact
    14101 aatgatgctt ttatagctag ttatactcat agaaacttat ttataaaata cagaaaaata
    14161 tttatagagt tagcaagacg aactgattca ataaaagagt cttttaatag aataattgaa
    14221 gaaatagcta taatcagagg agatgataac aatgtattct aaaatagcga ttaaaaatgt
    14281 tagaaaaagc tttaaggatt atagtatata ttttttaact ttaacattag taatttgtat
    14341 tttctatagt tttaactcta tggaatctca aaaagcttta atggaaatga gtacttcaaa
    14401 aaagcctgat ttagaaatgt ttaagaatat gatttcatat atatctgtgt tcgtatcagc
    14461 tattttatgt actttaattc tttatattaa taacttatta atcaaaaaaa gaaataagaa
    14521 acttggaatt tatatgactt taggaatggg aaaaattgaa atgtcgaaaa tattagtttt
    14581 tgaaactata atagttggaa tagtctcttt ggtaagtggg ctaatacttg gattattagt
    14641 atctcaaggg ttattaatac tattattaaa gttatttgaa tttaatatga gtgaatatag
    14701 ctttgtaata tcaataccag caattattaa aactattgtc tattttggaa taatattttt
    14761 acttgtaatg atatttaata tctatttaat ttctaaatac aaaatgatag atttattaat
    14821 tgtggaaaga aaaacagagg atataaaatt taaaaatcca tttgtatata tgattacatt
    14881 tgtatcatgc tttattttct tatttactgt atataacact acaaatcata tcataattaa
    14941 tctaatagat gataaacaat taaactccaa gtctaaaggt gtagaaataa atgaatttac
    15001 atatcaagat tggcaacaat tttgtgatat aaagtattat ggaaagaaag gtttaatatc
    15061 aatgacttta aaagaatata tagaaaaata caagtagaat aaaataaatt ttagatggaa
    15121 ggcattgccc tctatatttt tgtgttttct agaattgagt aaattttgtt actctagcaa
    15181 agtataaaag aaagaatgct ggtcttgagt attttatatt a
//
LOCUS       DAGSRM010000094        14991 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.094, whole genome shotgun sequence.
ACCESSION   DAGSRM010000094 DAGSRM010000000
VERSION     DAGSRM010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14991
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.094"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(182..565)
                     /locus_tag="MJE33_002990"
                     /pseudo
     CDS             complement(182..565)
                     /locus_tag="MJE33_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      806..903
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            958..1134
                     /locus_tag="MJE33_002991"
     CDS             958..1134
                     /locus_tag="MJE33_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648911.1"
                     /translation="MFGNKMDKCTHVLTAYISSSYDYCNFLDTQLDDFILEYGESVVE
                     SCLHQVMVLVSKYN"
     gene            1360..1560
                     /locus_tag="MJE33_002992"
     CDS             1360..1560
                     /locus_tag="MJE33_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBY9648912.1"
                     /translation="MNNGIVKWFNNEKGFGFISMEGRDDVFAHFSAIQTSGFKSLEEG
                     QQVSFDIVKGARGPQAENITIL"
     repeat_region   1925..2092
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1925..1952
                     /rpt_unit_seq="tttacataccacatagttgatataaaac"
     gene            2563..3408
                     /locus_tag="MJE33_002993"
     CDS             2563..3408
                     /locus_tag="MJE33_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648913.1"
                     /translation="MNIIKSITLLLTLLIVILMTGCSPRESASEVAKKYIDKMNSGDY
                     ETAYSLLSKDSKKKISIENLEEFQNILVSTKRIISYKIGKEKIIKKYKHDGKEYKNVV
                     KLEETFNIKNLLYKKDDSLKVNRYFVVENNKYKLLLYESFKKEFGINSMDLAQLKLND
                     FGLKYFKEVDLILKKAIAINPTDSKLYYAQACNYMNFDGFPGYSDTTYNALDSINKSL
                     KYLDKNDSDYKKNASNIYNLKGVILMFHKRFDEAQQYLNKSLYFNKDNEDPKYNLNTI
                     KKHLN"
     gene            complement(3632..3796)
                     /locus_tag="MJE33_002994"
     CDS             complement(3632..3796)
                     /locus_tag="MJE33_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648914.1"
                     /translation="MFGKPKESVRFLDFNDVAEKFGEKKAVAIFDAIAGKKSKEHIIK
                     DIEKLVKNEV"
     gene            complement(4338..4670)
                     /locus_tag="MJE33_002995"
     CDS             complement(4338..4670)
                     /locus_tag="MJE33_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBY9648915.1"
                     /translation="MNTREMKEYRVFYNKKLSETDEFFRVFGSLDDKTYSEGAISKKN
                     KELMGLAISILSRCDECICYHIEGCINSGASKDEIIEAIKIGVIGGGSITYPNARFAI
                     KILEEFNI"
     gene            complement(4914..5555)
                     /locus_tag="MJE33_002996"
     CDS             complement(4914..5555)
                     /locus_tag="MJE33_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBY9648916.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYNRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVIIQI
                     MMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKRNYSVGIILPWTLNDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFIYSYLMYKISGKNN"
     gene            complement(5536..5829)
                     /locus_tag="MJE33_002997"
     CDS             complement(5536..5829)
                     /locus_tag="MJE33_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBY9648917.1"
                     /translation="MGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATIS
                     YHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFMEKGEKDNEKSDK"
     gene            6222..6272
                     /locus_tag="MJE33_002998"
     CDS             6222..6272
                     /locus_tag="MJE33_002998"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648918.1"
                     /translation="MTEYIYYYNNKRIKKN"
     gene            complement(6798..7550)
                     /locus_tag="MJE33_002999"
     CDS             complement(6798..7550)
                     /locus_tag="MJE33_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBY9648919.1"
                     /translation="MFSVIFLCIGGFLAAFVDSIAGGGGLISMPVLMAIGVPVHLAIG
                     TNKFAASAGCISSAYRYAKSGKINNDLLKKLVPFTIIGSVLGVRCVLSISEEILNVLV
                     VVMILIVAIYTFISKNLGQEDNFEAVNKKNLRLGMLMALIMGFYDGFFGPGTGTFLTF
                     GFIKIYGYDFLHASANTKILNLTSNITSLLLFMINGQVDYKIAIVFALVMIMGAYVGA
                     KVAIKKGSKMIKPIFLVMALFMVVKLVYQTLV"
     gene            complement(7609..9264)
                     /locus_tag="MJE33_003000"
     CDS             complement(7609..9264)
                     /locus_tag="MJE33_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY9648920.1"
                     /translation="MVDFSESNNEVQKFDWGEVMWIHEPSKTQFNRLSAGIVRFFPGN
                     CQEKHFHLSEEQLLYVIQGEGIQIIDGKKVNIKETSIVYCPPYSEHEIINTGKIDLVI
                     LITYVPHKFSSLRQIPIVFSENNIQELVNVNIIENLANQISNILKLRISVYDLKYKEI
                     FETKKENKFCDICKNIKQCTKKLVKSINNDNFEKVYHCEYGVSSLEIPIVLEENIVGY
                     IECGNFIVYKSIDIDKNLSELSSSSGIDIKYINKIYNEFPLNPKSRLYVLKENLLMMS
                     EFIQEIAKRNFFENQLSIKDEEILKSRKENIRLEEALKKANSRLLEEEYIINPIDMFN
                     NSSKEYPFELELYIEKEIKNMNLEGVYNLIDTNKLRYNDVRDTIQEMIFVLSRTVLRD
                     LEDLKLISYLRNKYNKKISLVNSDEDLWNVLFEFSKECIDKNRAFWRKDKGNLIENIN
                     EYIKKYYKENINLNSISDVFFISPNYLSSIFNERNKVSITEYINLLRIEESKKYLLDR
                     SMSISDICKKVGFNNSSYFSQIFKKFNSITPNEYRKNMLDNKY"
     gene            complement(10537..11616)
                     /locus_tag="MJE33_003001"
     CDS             complement(10537..11616)
                     /locus_tag="MJE33_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBY9648921.1"
                     /translation="MILITLCILVAVFVLAFAGLRLIAPTIIRTSHKIEAPGIDRMEM
                     VEIGGIQQALYIRGQNADNPIILWVHGGPGNSMMPFLHLYQYEWENDFTVVNWDQRNV
                     GKTYFANDPAAVLQTMSAERVLQDVHEVTAYLKQEFNKEKIILIGHSWGTVLGTMAVQ
                     TYPEDYSAYIGIAQTVNMVDNERVGYEKTLEMVKAAGKQKDIKALEAIAPYPPAKYGP
                     SYLAALMTVRTYQSKYGLSINMDLGNIISILATPYYSLRELKNTAIVNQNAIDNQGDV
                     LRFLVEEADIRNYGVDYQVPVYYIMGENDYNSPYLPAKKFFDEIVAPDKQIFFIPDAD
                     HLPFLSKKAEFDCVLLEEIAPKLAK"
     gene            complement(11700..12356)
                     /locus_tag="MJE33_003002"
     CDS             complement(11700..12356)
                     /locus_tag="MJE33_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY9648922.1"
                     /translation="MTRKNNPKQAIENIITISAKLFAEKGYDKTSMQNIVDASGMSKG
                     GIFHHFSSKEDIFNVVMERRFEQIIETVNQWLGEMHGLTAKEKLRSLVRRHLTDEVIK
                     ESSNMITSAIESPHIILAFTQDNLKKLAPILANVLREGIEDRSIITEFPDECAEVILL
                     LFNFWCDTDVFQGDFPTLRKRFQFLQFLMRQIGVDILEDEIIESINIFYERKPMGQVE
                     "
     gene            12678..12884
                     /locus_tag="MJE33_003003"
     CDS             12678..12884
                     /locus_tag="MJE33_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648923.1"
                     /translation="MISHAAEICKKYKMPFIIFPTIEKKSIEEWVNIGANMLCLAQDT
                     TFITYVINQLLSFYNEIVPIQKQH"
     gene            complement(13037..13465)
                     /locus_tag="MJE33_003004"
     CDS             complement(13037..13465)
                     /locus_tag="MJE33_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBY9648924.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFVAEGVKLKGKTPEVLDVSLLKPS
                     DLKEEDKFALGCPSMGAEQLEEGYMEPFVSELESMVSGKQIGLFGSYGWGNCEWMRDW
                     EERMQNAGATIIGGEGITAMEDPNEEAKDECIELGKTLAE"
     gene            complement(13941..14879)
                     /locus_tag="MJE33_003005"
     CDS             complement(13941..14879)
                     /locus_tag="MJE33_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBY9648925.1"
                     /translation="MNKRMKLIPYEINENLRGAKNKFPYGIKQMNARGMWDEGYTGKN
                     IVVGIIDTGCDISHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNYN
                     NEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKNLK
                     NAVMQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNSN
                     TTIDLVAPGRNIISTYMDNKLAIMSGTSMSAPYVSGSLALIKEWAREEFERDLDEAEL
                     YAQLIKCTRALGIPRTEQGNGYLYLNLYKYKNNGKR"
BASE COUNT         5186 a         2450 c         1606 g         5749 t
ORIGIN      
        1 tatatatatg gctatttttc aacattttac aatatatata acatttattt ttttaacttt
       61 tgtcagtaaa atgtcagcaa aacactttat ccacaatttt tatataagtt ttccacaact
      121 tatccccagt attatccaca taaaaaaggt agctcttata ttattagcta ccttttattt
      181 attaatcttc atatttccag ctttttatcc aacttcctat ttctattatt ttttcttcag
      241 ttatttcttc tccatcatgt aattttgaaa ttaattctga taaaggattg ttatgtaaac
      301 ctctaaaaac tcttttttcc tctattttga tatattctct ctcttttatt aatactgtat
      361 agtgtacatg cctcccaatt ctctatgata ttaaaaaacc ttttttggtt aatctagcta
      421 aaagtataat tgtagttgta tgcttccaac catatgtttt ttctatttca tctgcaacat
      481 cttttgatat tacagtttta taaccagtat tccatatgca tttcataatt tttaattctg
      541 tatctggtaa ttttttattt agcataatat attccttctt tcaatttatg tcacttcttc
      601 ttatgtcact tcttcaaatt tatctagctt aaattataat aggagctttt tagaacaaaa
      661 actatattta atttttacca ggtactctac gtgtttatcc tgtttttaag tgtaaaataa
      721 tgttcatatc tataaattta tttaactttc aaacaatcct gttgaataat aactaattct
      781 attgtattat tttagttgta gaataaaact aaatcggcaa aactagagaa atttagtgac
      841 gcaaagctat agggactaag acttatataa atatcttatg agttatgtca gccagttgcc
      901 aaagagatag tgttcttttt gtttttatga aagtttttta gggggataat aatttatatg
      961 tttggaaata aaatggataa atgtacacat gtattgactg cttatattag cagttcgtat
     1021 gattattgta attttttaga tacacaatta gatgatttta tattagagta cggagaaagt
     1081 gtagtagagt cttgtttaca ccaagtgatg gtattggtaa gtaagtataa ttagatagac
     1141 attgatttct agagaaccct ttttagtttt atctacattt atattaatat atagcaatgt
     1201 aaaacgttta aatatatttt taatattaag aataactaaa atgttaacta aaaaatataa
     1261 ttaagtagac atttctatta atctatgata caatagtatt tgtggtaata cacaattaaa
     1321 tcaagtatat cttgatatag gttaatagga gtatttttaa tgaataatgg aatagtgaaa
     1381 tggtttaata atgaaaaagg attcggtttt atatcaatgg aaggtagaga tgatgtgttt
     1441 gctcatttct cagctataca aacttcagga tttaaatcat tagaagaagg tcaacaagta
     1501 agctttgata tagttaaagg tgctagaggt cctcaagcag aaaatataac tatattataa
     1561 tatatagatt tttcgggaaa gactcttttt agagtctttt tttattgtta atatatgtaa
     1621 gataatcatt ggtgaagtaa ataaggtata ttgagtaatg ttcttgatag tttatatgct
     1681 aatgaatttc aaaaaaataa gcactcctat aaaaagagtg ctcttaatgt aaatataaac
     1741 cttcattggt ccagcctaat accccgtatt ctccaagttc taactctttt ttatatacat
     1801 tatcactttc agccatatct aacacaatca tttcttcttt agaatcaatt tgtaaaatat
     1861 gaggactgtg tgttgtgaca attatttgtg tttttgggag aattgttttt aatgtaataa
     1921 aaattttaca taccacatag ttgatataaa acgtaaatac accatctata aagggcaggt
     1981 gttaaaactc tttacatacc acatagttga tataaaactt aatatactta ggtattccta
     2041 ctggaaactt ttctctttac ataccacata gttgatataa aaccccaaaa taaacttagc
     2101 atctccaatg tttacacata tacaatgctc ttaaaattgc agtgaacgcg caatagtgca
     2161 attgataata cttattatat attctcaatg ccttatattt caactgctaa acctcaattt
     2221 gtcacaaata tcgctcactg caaaatttct atatttttat tatatcataa catataatta
     2281 atattttcaa ttaattttta catttaatta tgtttaaatt ctataataat gcaactttaa
     2341 tgtttctttc tggaactaat ataaaaacta tttcagaaag attaggtcat gcagatataa
     2401 aaataactat gaatagctac tctcagtttt tagaagagat ggacagagaa gcatttgaaa
     2461 atttgagcaa aatactattt attatatttt aaagaagatg acttattcaa tatttgatgc
     2521 tagaatataa ttaataaaaa tttaattata ggtgattgta ttatgaatat tattaaaagc
     2581 ataactttac tcttaacatt attaattgtg atattaatga caggctgttc cccaagagaa
     2641 agtgctagtg aagttgcaaa aaaatatatt gataagatga actctggtga ctatgaaaca
     2701 gcatatagtt tattaagtaa agatagtaaa aaaaaaatta gtatagaaaa tcttgaagaa
     2761 tttcaaaata ttttagttag tacaaaaaga ataattagtt ataaaatagg taaagaaaaa
     2821 atcataaaaa aatataaaca tgatggaaaa gaatataaaa atgttgttaa attagaagaa
     2881 acttttaata taaaaaacct tctttataaa aaagatgatt ctctaaaagt aaatagatat
     2941 tttgtagttg aaaataacaa atacaagcta ttattgtatg aaagtttcaa aaaagaattt
     3001 ggtattaata gtatggacct tgctcaactt aagcttaatg attttggact aaaatacttt
     3061 aaagaagttg accttatact aaaaaaagct attgctatta atcctactga ttcaaagctt
     3121 tattatgccc aagcatgtaa ttatatgaat tttgatggat ttcctggtta ttcagataca
     3181 acatataatg ctttagattc aattaataaa tctttaaagt atttagataa aaatgattct
     3241 gactacaaaa aaaatgcaag taatatatat aacttaaaag gagttattct tatgtttcac
     3301 aaaagatttg atgaagcaca acaatattta aataagtcct tatactttaa caaagataat
     3361 gaagacccaa aatataatct aaatacaata aaaaaacatc taaactagat atttaatttt
     3421 tgaatttata aaatattttt ttatgtataa ttcagcactg aatcataatc tctactaaac
     3481 taattacaag tttaaattat aaagacttat ttctatataa atattactct ataacaataa
     3541 gtttcttatt taaattttaa aatattatct aaaaatcata aaaaagaagg tataaagagc
     3601 taccctcaag ctcaatatac cttataaata tctaaacttc attttttact aatttttcta
     3661 tatctttgat aatatgttct ttagattttt ttccagctat ggcatcaaaa attgcaacag
     3721 cctttttctc tccaaacttt tctgctacat cattaaagtc taaaaatcta acactttctt
     3781 ttggtttacc aaacatataa cacctctcat acatttttat atatttatta taacttctat
     3841 agacttattt ttcaccaata aacgttagac aaattagtcc ctttattttt ttaaactagt
     3901 tttaccttca aaattcttaa caaaaacagt ttattctact ctataaataa aaatagacaa
     3961 tttaatttgt aggaaactga taatagaata cagtaaataa aactttgttt aatctatctg
     4021 aaaaagttat ctattaaaac tgctatgaat ttataaagaa tattctagtt cataaaaata
     4081 caaatatgta aatataatca ccttaatatt caatttattt aaaataaaaa atgtataaaa
     4141 gatttaataa tgacaatata ctattctcaa tacaatctat gatgtaaata atttaaaact
     4201 actatattat ttaaaaattt atatttatat atgcaattct aaaaacttaa taagaagtga
     4261 acttagaaaa taaagcttaa tttcaaagac ttaaatagac tattattatt agtctaaata
     4321 aactacgtct atcaaaatta tatattaaat tcttctaaaa tttttattgc aaatcttgcg
     4381 tttggatatg taatagaccc gccaccaatt acacctattt ttatagcttc tataatttca
     4441 tctttacttg ctccagaatt aatacaaccc tcaatatgat aacaaataca ctcatcacat
     4501 cggcttaaaa ttgaaatagc tagtcccatt aattccttat tttttttact tatagcccct
     4561 tctgaataag tcttatcatc aagacttcca aatactctaa aaaactcatc tgtttcgctt
     4621 aattttttat tgtaaaatac cctatattct ttcatttctc tagtattcat cttctacctc
     4681 ctatgttttt cttataagaa tatcacactg tacttatatt ttcaatatta aatacaaatt
     4741 ttcattttat gttcattagc ctaatttata ttattttttg aaatctcaat aagttatcat
     4801 ataagtaaga ttttttcaga actaaaatag aaatcttagt aatttcttta ttacctatct
     4861 cagttctgaa atatattatt cttatccaaa cattattata ttgattaatt atattagtta
     4921 tttttacctg aaattttata catcaaataa gaatatataa atggtagtat tacaattaca
     4981 aagattacaa agatattata aaaaggaata ttaataaatg cattaataat taataatatt
     5041 ccaccaataa gccaaatcca tccagctaaa tggtgtgtct tattccaatt atcttcatca
     5101 ttaagagtcc atggcaaaat tattcctact gagtaatttc gtttacattt aggaagatag
     5161 tttccaaata aaataactat tattcctaaa attaatcctg tataaaaatt agactccaaa
     5221 ctaactcctg ttgaatataa aaccatcatt atttgtataa ttactgaaag tatagcaact
     5281 ccccattttg ataaattttg tataacattt gaaaaattag ctttcttagg ctcattctca
     5341 agtctaaacc atgtatacaa atggatacat agcattaaca atggtacacc aaaagctgct
     5401 ataacctttg gagcataatt atctggattt ccataattat caaaatgaat tgcaacttca
     5461 tttggtaatc tattataaaa atatattgat acaataaatg gtataaaaca tattaaagta
     5521 gataaaataa attgtttatt tatcgctttt ttcattatct ttttctccct tctccataaa
     5581 ttgtgaaaac caaagcataa cctcttcgaa aactgaaata tttatttcgt agaaaataaa
     5641 gttcttatac tttgtttcaa atatgagtcc tgcctttttt agttgagata gatgataaga
     5701 aattgtagca ttcgtcatat taaatttttc actaatttct cctgctgaca ttttattctc
     5761 cctcaacata accaatattt ctcttcttat agggtctgaa agagctttga atgtttctgg
     5821 aaaccccaac accatcacct cgctatttag aatttcatct aaatagaata taacataaca
     5881 tccattttat ttcaatagct atttagaaat ttttctaaat agctatttct aaatagttat
     5941 tgaaaatttt catattaaga ttctcaattt acttaagtcg cttttattta atcttacttt
     6001 atctaatttt tttaacttaa caaaagtgta ttgcaccata attgttaaag caaaaaccta
     6061 atgactgaga tacttatata tataacaata cttttaattt taaattaaaa gtagctaaac
     6121 ccaatttaaa aagtagttaa gtcctattta aatagaaaag aatgaaacga gaaatatact
     6181 gtataaaagt acataaaaac atctactaat tagatacaac tataacagaa tatatttatt
     6241 attataataa caaaagaata aaaaaaaatt aatttagcat aaccttgtta aatatagatt
     6301 tgaatatgaa tttaaagtta tgtaaaatta aaaatacagg aatgaaaatt ccattcctgt
     6361 ataaaatcta acttttgagt ctcagtacaa tcttattttg agacaatctt ttttgtttta
     6421 tattttctta tatttaaaat ataacgcata tcttatgcaa catattcata tttttcttta
     6481 tattctttca tcgcatcatt ccaagcgtct tgtactgtta cacctctttt attagcaatt
     6541 ttttgtgcca gttcaattat cttcatagct cttcttgata tgttcataaa cattcctcct
     6601 acaaattacc atatatttca tatatataaa tcaaggttat atttttatga tatataatcc
     6661 ttctattgta agattacagt ttatatgtgt attatatatg aactatatta gaagataatt
     6721 agaattttta taaagctata tgattagatt aaatataaaa gctttacaat tacttataaa
     6781 tttaaaaagt ctataagcta aactaatgtt tgatatacca attttacgac cataaataat
     6841 gccattacta aaaatattgg ttttatcatc tttgaacctt tctttatagc aactttagca
     6901 cctacataag ctcccattat catcactaat gcaaacacta tagcaatttt ataatctact
     6961 tgaccattta tcatgaataa taacaatgaa gttatattac ttgttaagtt taatattttt
     7021 gtatttgcag aagcatgtaa aaaatcatat ccatatattt ttataaatcc aaaggtcaaa
     7081 aaagttcctg tacctggacc aaaaaaacca tcataaaaac ccattataag agccataagc
     7141 atccctagtc ttaaattttt cttattaaca gcttcaaaat tatcttcttg acctaaattt
     7201 ttagaaataa aggtgtaaat agccactatt aaaatcataa ccacaaccaa aacatttaat
     7261 atctcttcac ttattgataa tacacatctc acacctagaa cactaccaat aatagtaaat
     7321 ggtactaatt tttttaaaag gtcgttatta atttttccag actttgcata tctataggca
     7381 ctacttatac atcctgctga tgctgcaaat ttatttgtac caatggcaag atgtactggc
     7441 actccaattg ccataagaac aggcatgctt ataagtcctc ctcctcctgc tatagaatct
     7501 acaaaagcag ctaaaaaccc ccctatacat aaaaaaataa ctgaaaacat aaaattcctc
     7561 catcattctc tttcaatttt tcttacaata tatattatat aatcttattt aatacttatt
     7621 atccagcata ttctttctat attcatttgg agttatacta ttaaacttct taaatatttg
     7681 tgaaaaataa ctactgttat taaaacctac tttcttacaa atatcactaa tactcatgct
     7741 tctatctaac agatattttt tagattcttc tatcctaaga agatttatat actcagttat
     7801 tgaaacttta tttctttcat taaaaataga actcaaataa tttggactta taaaaaaaac
     7861 atctgatata ctattcaaat ttatattttc tttataatat ttttttatat attcgtttat
     7921 attttcaatt agatttcctt tatcttttct ccaaaatgct ctattcttat caatacactc
     7981 tttggaaaat tcaaataata cattccataa atcttcatca ctgtttacaa gtgatatttt
     8041 tttattatat ttatttctta aataagatat aagttttaaa tcctctaaat ctctaagcac
     8101 tgttctagac aatacaaata tcatttcctg aatagtatct ctaacatcat tatatcttaa
     8161 tttgtttgta tctattagat tataaacacc ttctaaattc atatttttaa tttctttttc
     8221 aatataaagt tctaattcaa agggatattc ttttgaagaa ttattgaaca tatctatcgg
     8281 attaataata tactcttctt ctaataatct actatttgct tttttaagtg cctcttcaag
     8341 tcttatattt tcttttctac tcttcaatat ctcctcatct tttatactaa gttgattttc
     8401 aaaaaaattc ctcttagcta tttcctgaat aaattcagac atcattaata aattttcttt
     8461 taatacatat aacctacttt taggatttaa tggaaattca ttgtaaattt tgtttatgta
     8521 ttttatatca ataccagaac tacttgataa ttcagataaa tttttatcta tatcaataga
     8581 cttatatact ataaaattac cacattctat atatccaact atattttctt ctaatactat
     8641 aggtatctct aaactggaaa caccatactc acaatggtac actttttcaa agttatcatt
     8701 atttatgctt ttaacaagtt ttttagtaca ctgttttata tttttacaaa tatcgcaaaa
     8761 tttattttct ttcttagtct caaatatttc tttatacttt aagtcatata cagatattct
     8821 aagcttcaat atattagata tttgatttgc aagattttca attatgttta cattaactaa
     8881 ctcctgtata ttgttttcag aaaatactat aggtatttgt ctcaaactcg aaaatttatg
     8941 aggaacataa gtaattaata ttactaaatc aattttacca gtgtttataa tttcatgttc
     9001 tgaatatggt gggcaataaa ctatagatgt ttcctttata ttgacctttt taccatcaat
     9061 tatttgtatt ccttctccct gaatcacata taaaagctgt tcttcactta aatgaaaatg
     9121 cttttcttgg cagtttcccg gaaaaaatct tacaattcca gcactaagtc tattgaattg
     9181 tgtttttgat ggttcgtgta tccacataac ctctccccaa tcaaattttt gtacttcatt
     9241 atttgactca ctaaaatcca ccataaactc acctcgtagt ttttgtctaa atattttttc
     9301 ttcccttctt atattaaaat aatatcatca tcattaactt tcattactat aaaatcatca
     9361 tactctttta atactcctgt aattttagtt gcacttagtt tactacaatt cagtttttta
     9421 atatattgat aaattctata attacttttt tattttgtaa aatcagtgtt ttcccaaaat
     9481 aattatatct ataaatgaaa gtatttttac tgcttacatc aatatatgtt atcataattt
     9541 tcctttttat tgcatatata aataactgta tataacattg tccaatacta tatagaaata
     9601 catctaagaa agaatattat taaattgttc caattagtat tatttttctt ataaaaataa
     9661 aaaaactgtt ggattctttt agaaaaatta ttttttacat accttttttg cttagatagt
     9721 ctatcttctc agctattttg tatatacact cttctagttc atgattttat tttaatatct
     9781 tgcttatatt gctgaaataa ttgaattctt ttatctatca tattgtctct tatatcataa
     9841 acatattgtt tcttatatca caaaaatgaa agcattctta tatcttcaat atataaacct
     9901 gcttttttta atcttgtaat aaatatagga tactctatat tctcttccta gaaataacta
     9961 ataccactta tttttctatt atatagaaag taataaatct ttttcttaat aaaattcaat
    10021 agtaagtgga ataaaatttt atatgaataa gaacattata aaaatacttt atacaatagt
    10081 caataaaaga tattagcttc aaattatgtt aaagttgtat tatttaaagt gaaaaaacaa
    10141 ataaaaaaga aataatagta taagtagaaa aatatactta aaacaaggcg aagcctctct
    10201 tgtatctaaa tcaagtagag aattaaatct aaaaaagaaa ataaatataa aagatgactc
    10261 ctgtacatta caagaatcat cttatggttt cataatcact ccatggtttt taactgtctg
    10321 atataaaggt ctcagttcac tcatagcatg accacccttt ccgattgctg accccgtcac
    10381 ttatggacaa aatgtccata agtgatagtc ctctcttaaa accctctaaa tagatatatc
    10441 tcaagcacct cattgaaacc agttgctgga aacaaactga agcgttctgg aatagtaaaa
    10501 gaaattgcca ccatatccac gttactgtcc tagttgttac tttgctaact tcggagcaat
    10561 ttcctctaac aatacgcagt caaattctgc ctttttgcta agaaatggca gatggtctgc
    10621 atctggtata aaaaaaatct gtttgtctgg cgccactatt tcatcaaaaa atttcttcgc
    10681 tggaagatag gggctattat agtcattttc tcccatgatg tagtatacag ggacctgata
    10741 gtcaactcca taatttctta tgtcagcttc ttccactaaa aaccgcaata catcaccttg
    10801 attgtcaatc gcattttgat tgacaatggc cgtatttttt aactctctca atgaataata
    10861 cggggttgcc agtatactga ttatattgcc caaatccata tttatagaaa gcccatactt
    10921 gctttggtat gttcttactg tcataagagc tgccaaatag cttggaccat attttgctgg
    10981 cggatatggt gcaatagctt ccagcgcctt gatatctttt tgcttccctg cagctttcac
    11041 catttcaagt gtcttttcat agcccacgcg ctcattgtcc accatattaa cggtctgcgc
    11101 aatcccaatg tacgcgctgt aatcctccgg ataggtttgc acagccatag ttcccagcac
    11161 tgttccccag ctatgaccta taaggataat tttctccttg ttgaactctt gcttcaaata
    11221 tgctgtcacc tcatggacgt cttgcagaac acgctccgcg ctcatggtct gcaaaactgc
    11281 tgccggatcg ttcgcaaagt atgttttgcc tacgtttcgc tgatcccagt tcaccacagt
    11341 aaaatcattt tcccactcat actggtacag atgtaaaaat ggcatcattg aattaccagg
    11401 acccccatga acccaaagga tgatggggtt gtctgcgttt tgtccccgga tatataacgc
    11461 ctgctgtatg ccgccgatct ccaccatttc catgcggtcg attccaggag cttctatttt
    11521 atggcttgta cgtataatcg tgggggctat cagacgcaaa ccggcaaacg ccagaacaaa
    11581 aacagcaact agtatgcaaa gtgtgattaa tatcatttta agtaccttct ttcatgaatt
    11641 tttttagcca tagatatctc ctctaacatt aaaaaaaata tgttgctatt ttagttttgt
    11701 tactctacct gtcccattgg tttcctttca taaaaaatat taattgattc tataatctca
    11761 tcttccagga tatcaactcc tatttgtctc ataagaaact gtaagaattg aaaccgttta
    11821 cgaagtgtag gaaagtcccc ctgaaataca tctgtatcac accaaaagtt aaagagcagc
    11881 aaaattacct ctgcgcattc atctgggaac tctgtgatga tgcttctatc ctctatgcct
    11941 tctcgtaaaa catttgctaa tattggtgct agttttttca aattatcctg tgtaaatgcc
    12001 agtatgatat gcggactttc tatagcgctt gtaatcatgt tactagattc ttttatcacc
    12061 tcatccgtta aatgccgcct aactaaactt ctaagcttct cttttgctgt aagtccatgc
    12121 atttcaccga gccattgatt tactgtctct attatctgtt caaagcgcct ttccatgacc
    12181 acattaaaaa tatcttcttt tgaactgaag tgatgaaaaa ttccgccctt tgacattccc
    12241 gaagcatcaa caatattttg catacttgtt ttgtcatatc ctttttcagc aaacaatttt
    12301 gcagaaatgg taatgatatt ttcaatagct tgctttggat tgttctttct agtcattata
    12361 taactccttc ttattaaaaa ccgactgttg gtttttatta tataagctat tcctacttct
    12421 gtcaagcaac attatgttta tggcaaaaca ataaggtttg gtggttagac aggtattaat
    12481 tagactaatg cgactttccc ctagatggaa cccttggtaa atttgcaatc tttaccggct
    12541 ttgattatat ctgtaagcaa ggcaggtatg gccacacctg ccctgtttga ccctcggata
    12601 ttcattccta tcccttctct ccttagccat tacaccaaaa cgccgctcat tgtctccaat
    12661 ggcggcgttt taaagatatg atttctcatg ctgctgaaat atgcaaaaaa tataagatgc
    12721 cctttattat ttttcctaca atagaaaaga aaagtattga agaatgggtt aatattggag
    12781 caaatatgct ttgtttggca caagatacta cctttatcac ttatgttatc aatcaattat
    12841 tatcttttta taatgagatt gtaccaattc aaaaacaaca ttaaattata aatctaattt
    12901 tatattaaaa gagactaata ttttatctat ctctgttaag gatattttct tatgtatcat
    12961 aatattagtc tctcttatta ttatatactg ctatcatcat tagttgctat ttatactatt
    13021 tttataatat acaaatttat tcagctaacg ttttgcctaa ttctatacac tcatcttttg
    13081 cttcttcatt tgggtcttcc attgctgtaa ttccttctcc accaataatt gtagcaccag
    13141 cattttgcat acgttcttcc cagtctctca tccattcaca atttccccat ccatatgaac
    13201 caaataatcc aatctgttta cctgatacca tagattctaa ttctgaaaca aatggctcca
    13261 tatacccctc ttctagttgc tctgctccca tagatgggca acctaatgca aatttatctt
    13321 cttcttttaa atcacttggt ttcagtaagc tcacatctaa aacttctggt gtcttacctt
    13381 ttaacttcac accttcagcc acaaaatttg ccattttttc agtatttcct gttccactcc
    13441 aataaacaat atatatttta ctcattttta cctcctgtag tattttttaa ttaatcaata
    13501 gttagttata gctaactaac atctaaaaaa caaaagattt ctttatctga aatctcttta
    13561 tttttctaaa tatagaatat catagttgat tttgaaattc aatgcttatt gataatattt
    13621 atcatttata ttttatattc attttataat atcaaaacac tgcatcgtat ttctaatctt
    13681 tcatagaaaa atttctaact tatagtactt ttgcatctct tataaatctt ttatatgtat
    13741 aagtacatag aaaaacacac cttttccaga tgtgctatct ataatatcgt atatttattt
    13801 ttcaatttta actagctaat atgtttataa attttatcac ttaatacctt tatacattga
    13861 tgtaaaatct tacacctaag taatttttat tatttgtctc gactctatat aatatagcta
    13921 ttttatcatc aaatcaaaaa ttatcttttg ccattattct tgtatttata gagatttaaa
    13981 tataagtatc catttccttg ttccgttcta ggtattccaa gcgctctcgt acattttatt
    14041 aattgtgcat acagttcagc ttcatctaaa tctctttcaa actcctctct tgcccattct
    14101 ttaattagtg ctaatgagcc tgatacgtat ggtgcactca tactggtacc actcataata
    14161 gcaagtttat tatccatata agtcgatata atatttcttc ctggagctac caaatctatt
    14221 gtagtatttg aattactaaa cttttcaacc aaatagtttt catttattgc acctacttct
    14281 attacctcag cataactggc tggataacta tactcacttg tactagaatc accatctcca
    14341 ttattacctg ctgcacacac tacagaaata ttatttttta ctgcttgcat gacagcattt
    14401 tttaaattct tatcatcttt gtttccacca agagacatag atataatatc aaccttgtta
    14461 tttacagcaa agttaatagc attaattata ctttgatatg ttccagtacc atctttattt
    14521 aatgcttttg ctattaataa tttacaatct ggagctactc ccataacttc attattatag
    14581 ttagatgcag ctataatacc cgccacatga gttccatgac cattaaaatc ctcatatata
    14641 tttttatttc cattactgtc atcactaaaa tttgcaccac caattatttt tccttttaaa
    14701 agaggatgag atatatcaca acctgtatct attataccaa ctacaatatt tttaccagta
    14761 taaccttcat cccacattcc cctagcattc atttgtttta ttccatatgg gaatttattt
    14821 tttgcacctc ttagattttc atttatttca tacggaatta gtttcattct tttattcatc
    14881 atatatcctc ctaaaaattt tattgcttat aaaaatcatg aattttattt tataataggt
    14941 ttataaatca aattaaaacc tatatctact tatttatatt tatgatatat t
//
LOCUS       DAGSRM010000095        14896 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.095, whole genome shotgun sequence.
ACCESSION   DAGSRM010000095 DAGSRM010000000
VERSION     DAGSRM010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14896
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.095"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            1097..2767
                     /locus_tag="MJE33_003006"
     CDS             1097..2767
                     /locus_tag="MJE33_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HBY9648926.1"
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINRNKFKISSAI
                     FDEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLHGKKGEHNLEYRLINKYKEI
                     VWISCRGKVYVSDDPKTIFLVGRIKNIGEKNKFDSITGTWNREQFEHRMNYLIKEKIY
                     KNGAMFIMDIDNFKNINEKYGHSYGDKVLRAIATEVLEYLPKDVRLYRLDGDEFAFFY
                     PMCTKETIEKIYEKIQMYTNTQHEIESNKYYCTVTAGVAMYPEDGDNYLDLFKHADIA
                     LDIAKISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQPQVNAVTKE
                     VIGAEVLLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDF
                     KISVNVSYIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLK
                     GIGVYIAIDDFGTGYSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHI
                     INLKVCVEGIESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKFQLSK"
     gene            complement(2889..3623)
                     /gene="pflA"
                     /locus_tag="MJE33_003007"
     CDS             complement(2889..3623)
                     /gene="pflA"
                     /locus_tag="MJE33_003007"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBY9648927.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNTDLVLLDLKHMVEEKSIDLTGVGMDKALKLARHLESRNIP
                     VWIRHVLVPGITDDIDNLEKLGQFVATLKNVERFELLPYHSMGIHKWESLGIDYELKD
                     VPDATKEDVQKASEIISKFGVKVYNS"
     gene            complement(3783..6014)
                     /gene="pflB"
                     /locus_tag="MJE33_003008"
     CDS             complement(3783..6014)
                     /gene="pflB"
                     /locus_tag="MJE33_003008"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBY9648928.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTK
                     KLWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPAALGKDMTERINNLSAMMDGYFA
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            6307..7368
                     /locus_tag="MJE33_003009"
     CDS             6307..7368
                     /locus_tag="MJE33_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HBY9648929.1"
                     /translation="MEKRKSIHLHKLNIYLIGGGDRLFITVVLFLVGFLLITKGADIF
                     INCTVEIGKKTNISEIILGATIVSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICN
                     TGLALGLVVFISPFNVDKKMFFSKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIF
                     YMVNNYRSVVGKSDTKNRNIKNNTIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMM
                     IIGSQILIESGVIIASFLNIPQGIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNI
                     LGANILNIVSVIAVSAIPNNIPILSQNRQLDIPFMILLLLIVIIPTLKSNKLSRIQGI
                     LMLFTYFLYISILYFMYII"
     gene            7459..9072
                     /locus_tag="MJE33_003010"
     CDS             7459..9072
                     /locus_tag="MJE33_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBY9648930.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
     gene            9347..11332
                     /locus_tag="MJE33_003011"
     CDS             9347..11332
                     /locus_tag="MJE33_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBY9648931.1"
                     /translation="MSMTYMDPRVFLILEMVLEEDTNTLEQFSNVLGVSTRTIRNYIN
                     QINRELKGGIAKIVKNEKGTYSIRIEDESKLIEIVNFNRGKLSNFINLNSPDERINYV
                     LDILTMTNNPITIDDLAEEICIGRTTLIKDLKKADRILAEYDLELKRKPNIGMILVGE
                     EMDIRLLILDRLYENYIDVLENVNQINLVKDYDIECLRDELKKLFKKEELYITEQVLR
                     DVERYIIMSVLRNLNGYKFEKIDKRFEVIRCSDEYTLGLKLKALLEKIFNIVLNEKET
                     IFLTMPLIGRKAPSTKLALSSIKINDSVKEVVDEVTSFLLETSGIDFKEDKKLIENLE
                     YHLYFALNRMRFNIRVKNPLLQEIKERYTFPYSVAKIAAMIIEEKFDLKICDHEIGYI
                     ALHFGSYFEKNSRKVLNVNRVAVVCGTGLGTAQLIRIKIGKILGESVEVNTFSDIEAK
                     ANLDLLKYYDLVFTTVDFKTDLDVPIFRVNALFNEESIKKEIENAILLKNSINLKSIK
                     KIEKNIPFIGKLIEEDKFFILNKSTFMENLEDMLEKLVHKGIIDIEFKKKVLKREKKS
                     STALDNYIALPHSVNTNGENLYLAFGILEKPVIWDTKEIRIIILMIIPDKNVDSTDLI
                     IKSYEEVLEVGRDKKMVEELAKVKSFDEFYKIITKRR"
     gene            11337..11771
                     /locus_tag="MJE33_003012"
     CDS             11337..11771
                     /locus_tag="MJE33_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HBY9648932.1"
                     /translation="MDPVIFIIASLITVYLLNIFLGYLQLKDFNKNYIELKRKGRVAI
                     GRKKGRISSGTIVLILINDDGVIVETRKMQGVTVLARVKSFEGLVGKSLEYIEKSDLT
                     VYNKLLKKAILDAVKQYITFEKNKCEDTNNLSTDEECRMEVV"
     gene            11800..12360
                     /gene="srlA"
                     /locus_tag="MJE33_003013"
     CDS             11800..12360
                     /gene="srlA"
                     /locus_tag="MJE33_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418408.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBY9648933.1"
                     /translation="MTQFFQVLASGADSFMNLFRAGGQQFVGFVTDIVPLLVALLVTM
                     NAIINFIGTDRVERLAKKCSANIFTRYLILPVLGTFVFANPMTLSLGRFLPEKFKPSY
                     FAAASFSCHTMNGLFPHINPGELFIYLGIANGITTLGFSTADLAVRYLLVGIVANFIK
                     GVVTDYTTKFVEKQQGIALNSDIKIG"
     gene            12424..12792
                     /locus_tag="MJE33_003014"
     CDS             12424..12792
                     /locus_tag="MJE33_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIB"
                     /protein_id="HBY9648934.1"
                     /translation="MAFNKVKIVKGSGGWGGPLIIEPTEKKNKVVYVTGGAKPETAVK
                     IAELTGCELIDGFSQGVKDDEIACVIINCGGTLRCGIYPQKKIPTINIMKTGKSGPLA
                     MFIKEDIYVSGVKPTNVEQL"
     gene            12817..13383
                     /locus_tag="MJE33_003015"
     CDS             12817..13383
                     /locus_tag="MJE33_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBY9648935.1"
                     /translation="MAYDTKKKISEQSQGIVAKIGIALGNVTSTLFQAGRDTIDTLIH
                     TILPFMAFVAVLIGIINQSGIGNWVANLLTPLIGNVGGLVVLSIICSFPLLSPFLGPG
                     AVIAQIVGVLVGTEIGKGNIPPSLALPALFAINSQAACDFIPVGLGLAEAEPDTVQVG
                     VPSVLYSRFLTGAPTVLLAWLASFGLYK"
     gene            13424..13789
                     /locus_tag="MJE33_003016"
     CDS             13424..13789
                     /locus_tag="MJE33_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBY9648936.1"
                     /translation="MELIYSTQINKIGDNANEFLQHNMFVTFKDNAPQELENYCYIHS
                     ENNLVKEILTDDVLSINNVDYKITSVGELVNQNLSELGHITFKFTGEKDGAIGGTLYL
                     EEKEIAPIGVGTILKIIRK"
     gene            13890..14663
                     /gene="srlD"
                     /locus_tag="MJE33_003017"
     CDS             13890..14663
                     /gene="srlD"
                     /locus_tag="MJE33_003017"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HBY9648937.1"
                     /translation="MRKVALVVGGGRSLGEYLSKHLAKVGYDVAIADLNEDNAKKVAE
                     EIKTEFGCKSVGYSCNAIDERQVISTVKAIENDFDRIDLLVYNAGVAVSKKITDFGYK
                     DFKFIVDVNLNGYFLFAREVSKVMIAKGIEGRIIQINSKSGRVGSKHNSGYSAGKFAG
                     VGLTQSLALDLAEHKITVNALMLGNLLDSDMFESLIPQYAVKLGIPESEVKQVYIDKV
                     PLKRGCRFIDVANMLTFYASKEAEYCTGQSINITGGQVM"
BASE COUNT         5738 a         1738 c         2478 g         4942 t
ORIGIN      
        1 tgaaataggg aaaaaatagt gatataataa gaaaaaatag agaaaaaaag taacccttag
       61 ttataaaaaa gttactattt attgaaaatt tagatatttt tctaaaaatt atctatgtta
      121 atagttaaat ttatgaacat tttttgtttt tatgttagga tatttcttag aaatgaaata
      181 cttcttaatt tgatttgata tgtatatttt tatagctatt ttattataaa aaaaaagaac
      241 ccttttataa caaatggaaa ctatggatgt aataattaaa ataaaaaata ttattttata
      301 tttataaaca ttgacattct aattttgtag tgtaatcgta ttttttatta attcttacaa
      361 aatcaatgaa aataagtata tccattttaa agtacataaa tttaaaaatc cagaaaaata
      421 tacttaaatt atgtaagtaa aagaggggtt gaatcagttg aaaatgtaat attagtaaat
      481 agtacatcaa tcctatatac cctgtaagca actctatttt catgtactaa aaatgtacct
      541 attttattaa ctgaaaagaa taaattaaat gtaagtacat aagaagatag aaaaaattaa
      601 aaacaaaaaa tatttatata aataaatata aaaaatgata taatccctat taaaaaattt
      661 atagatgatg aagaaatatc aaaatcttat attataggaa gagacacttc tttgccaaaa
      721 tctatagaaa agcaatacca aatccaaaga gaataggcga agaaaaaaat aaaataaatt
      781 caagagtaat agaaaagttt tatagaataa agaactgata actctttata taacgaaaaa
      841 tagaatttta agagaaaatg atttaataaa tgatttaaca tggtgtttta gcaggtaaaa
      901 ataaatcacc agtaatgatt atagaaaaat tactttatga atctcaaaaa acttagttaa
      961 gagtaagaga tttgataaga taactaaagt tggggaaatg tgtttgatga gacaaaaaat
     1021 tagtaaaata gtataatcta aataaggata ttttgttgat taaactacac taatagtaat
     1081 attgtagggg gaaaagatga gagaagaatc gaactcttta agtttagacc atatagaaat
     1141 tttctttgaa cttttatctc aaagtacaga agattatata tttttttggg atataaatag
     1201 gaataaattt aaaatttctt cagcaatttt tgatgaattt aacttaagta aagagataga
     1261 atctgatttg gtcaattgtt ggagtaaaat tgtttatccg gatgatgttc aaatatggaa
     1321 agatgatata caagaattac tacatggaaa aaaaggtgag cacaatttag aatataggtt
     1381 gattaataaa tataaagaaa tagtatggat ttcttgtaga ggtaaagttt atgtgtcgga
     1441 tgacccaaaa actatatttc tagtcgggag aataaagaat attggtgaaa aaaataaatt
     1501 tgattctatc acaggaacgt ggaatagaga acaatttgaa catagaatga attatcttat
     1561 aaaagagaaa atttataaaa atggtgctat gtttattatg gatattgata attttaaaaa
     1621 tataaatgaa aaatatggac attcttatgg tgacaaagta ttgcgtgcaa ttgctactga
     1681 agtattagaa tatcttccaa aagatgtaag attgtatagg ttagatggag atgaatttgc
     1741 atttttttac cctatgtgta ctaaagaaac tatagagaaa atatatgaaa agatacagat
     1801 gtatacaaat actcagcatg aaattgaatc aaataagtac tattgcacag tgactgcagg
     1861 cgttgctatg tatccagaag atggagataa ttatttagat ttatttaaac atgctgatat
     1921 tgcattggat atagcaaaaa ttagtggtaa aaatagaatt aagttttttt cacaagagct
     1981 ctatgaaaat aaactaaagg taatttcaat gcaacaaaaa ctaagagagt gcgttgaaaa
     2041 taattttaat gactttgagc tgttttttca accccaggta aatgctgtta caaaagaggt
     2101 aataggggca gaggtacttt taagatggca ttcgagtact tatggagaag tatctcctgt
     2161 tgaatttata ccaatactag aacagagtaa tttaattata ccagttggga agtggattat
     2221 aaaagaagct gtaaaacagt gtaaagaatg gcataaaata aatccagact ttaaaatatc
     2281 tgtaaatgta tcgtatattc agttaaagga agattttttt agagatttta tagtagaatg
     2341 tttagtagag tatcaattaa gacctgaatt tttaattttg gaattaacag agaattgttg
     2401 gataccagat ataaatttac taaatgataa atttattagt ttaaagggta taggagtata
     2461 tattgcaata gatgactttg gtacaggata ttcatcttta aattacctta aagaactgtc
     2521 tgttaatata ataaaaattg aaagaagttt tgttaagaat attacatata atagttatga
     2581 atatactttt cttgaatata ttattaaatt agctcatatt ataaatttaa aagtttgtgt
     2641 tgagggaata gagtcttatg aagagtatga tatagtaaaa agcttagggg tagatattat
     2701 tcagggtttt ttatttggaa gacctgtaag tgcctctgaa ttttataaat ttcaactatc
     2761 aaagtgatgt atataaaatg agatatatag tatataaaaa tagggcaaag tggaagtttt
     2821 gaagtttatt aaaaatttac actttaccct attttgttat gtcttctata aaggattaat
     2881 atactacact aactattata tactttcact ccaaatttag agattatttc actagctttt
     2941 tggacatctt ccttagtggc atcaggaaca tcttttagtt cataatcgat acctaaactt
     3001 tcccatttgt gtattcccat agaatggtat ggtagcaatt caaatctttc tacatttttc
     3061 aatgtagcta caaattgacc tagtttttct aaattatcta tatcatcagt aatgccagga
     3121 actaaaacat gtcttatcca tacaggaata tttcttgatt ctaagtgtct agcaagtttt
     3181 aatgccttat ccataccaac accagtcaaa tcaatagatt tttcctcaac catatgtttt
     3241 aaatcaagta aaaccaaatc agtattatct aaaactgggt ctatagtttc tatatctaca
     3301 aatccagatg tatccaagca agtgtgtata tcattttctt ttgctttttt aaataattca
     3361 cttaaaaatt ctggttgaag agtggattca ccaccagagg cagttatacc tccaccagaa
     3421 aacttcataa aagaagtata cttaagagca tccgttatta tttcatcaac tgtgtattct
     3481 tttcctgatt taatatccca tgtatccctg ttatgacagt atttgcatct aagaggacac
     3541 ccctgaaaaa ataatatata tcttattcct ggtccatcta cagtaccaaa cgtttctata
     3601 gaatgtatct ttcccttaat catagagttc acctcacaat atattttaat aaccttatca
     3661 atagttataa tatttctcta aaaaaggcca tcttatacca gaaataattc aaatataaga
     3721 tagcctttta tttttatttt ttacacagta gtaatcttag ttaaagtata aaaatttaaa
     3781 gactaagcca ttttaccgtg gaatgttctg tttataacat ctaattgttg ctctttagtt
     3841 aattttatga agttaactgc atatccagat actcttatag ttaattgtgg atattcttct
     3901 ggatgttcca tagcatcttc taaagtagct ctatcaaata catttacatt taagtgatga
     3961 gcattttgag cgaagtatcc atccatcatt gcagaaagat tgtttattct ttcagtcata
     4021 tcttttccta aagcagcagg tactatagag aaagtattag atataccatc ttgagcatgt
     4081 tcatatggta atttagcaac tgaagataat gaagctaaag caccattagt atctctacca
     4141 tgcattgggt tagcaccagg agcaaatgga gcaccagctc ttctaccatc tggtgtatta
     4201 ccagttttct taccatagac aacatttgaa gttattgtaa gtatagattg agtaggataa
     4261 gaatttctat aagttttatt ctttcttatt ttattcataa agctttcaac taactctaca
     4321 gctatactat caactctatc atcattattt ccgtattttg gataatctcc ttctatttca
     4381 aaatcaacag ctattccttg ttcatttcta atagttttaa ccttagcatg ttttatagca
     4441 gataatgagt ctgcacaaac tgataatcca gccataccac aagccatagt tctaaatata
     4501 tctctatcat gtaatgccat ttctaatgct tcataagaat atttatcatg catataatgt
     4561 attacattta aagtatttac atataatgtt gctaaccaat cagtgaatgg ttcaaattta
     4621 ctcataactt catcataatc taagtactca gaagttatag gttcaaatct tggtccaact
     4681 tgaacaccag atttctcatc tacaccacca tttatagcgt ataataaagt tttagctaag
     4741 ttaactctag ctccaaagaa ctgcatttgt ttaccaattc tcattgcaga tacacaacaa
     4801 gctataccat agtcatctcc ccagtatgct ctcattaaat catcattttc atactgtact
     4861 gaacttgtat ctatagatac tttagaacag aaatctttga atccttgagg taatttagtt
     4921 gaccatagaa ctgttaagtt tggttctgga gaaggcccta atgtatatag agtatttaac
     4981 attctaaatg agtttttagt aactaatgtt ctaccatcta ttcccatacc tcctatacac
     5041 tcagttaccc aagttgggtc tccagagaat aagttattgt aatcaggagt tcttaggaac
     5101 ttaaccattc ttaatttcat aacaaaatgg tccatatact cttgtaattc ttcttctgtt
     5161 acgattccag cttttaaatc tctttcaaag tatatatcta agaaagtaga tgttctacct
     5221 aaagacattg cagcaccatt ttggtcttta acagcaccta aataaccaaa gtataaccat
     5281 tgtacagctt cttttgagtt agttgctggt tttgatatat caaatccata agattcagcc
     5341 atttttttaa gttcatttaa agctgatatt tgctcagtta tttcttctct taatctgata
     5401 acttcctcat ccatgcaaga aacttctaat gatttctttt gctcattttt atcttctatt
     5461 aaagcatcaa caccatatag agcaactctt ctgtagtcac caattattct acctctacca
     5521 taagcatctg gaagacctgt tattatacca gattttctag cagctctcat ttctggagta
     5581 taaacatcaa aaactccttg gttgtgtgtt tttctgtatt tagtaaatat atcttttatt
     5641 tctgggtcta attcatatcc aaaagcttcg catgaatttt caaccatctt tataccacca
     5701 tatggcatga cagctctttt taaaggggca tccgtttgta acccaactat tgtttctaat
     5761 tctttatcta aatatcctgg agcatatgca gctattccag aaacagtttt tgtatcaaca
     5821 tctaaagtac caccattttc tctttctttt ttaaatagga ccattgcttc gtcccataat
     5881 tttttagtat tttcagtggc accagctaag aaagagtcat ttccctcata tggagaatag
     5941 tttaattgta taaactctct aacatttatt tctttagtcc atcttcctgt tttaaatcct
     6001 tgccatgcat tcattaagta tttcctcctt agatttaaat gataaataat actattaaaa
     6061 ttaattttta tatacttaat ttattttata tattttttta aaaatatatt ttctaatata
     6121 ataatagcat attattcgta tcctgtatac ataaaaaata aaaaattgtc gctttaaagt
     6181 ataaaacaga ttatatgtta ataatgtggt gaaattatac ccatatataa ataaaataaa
     6241 acaaataaat agctaattaa ggagaaaata ttttttattt tttgtttagg ataaataaat
     6301 tttattatgg aaaaaagaaa aagcatacat cttcacaaac tgaatattta tttaataggg
     6361 ggaggtgata gattgtttat cacagtggta ttatttttag taggtttcct attaattact
     6421 aaaggcgcag atatctttat aaattgtact gtagagatag gtaaaaaaac gaatatatca
     6481 gaaattatac ttggagctac tatagttagt tttgcaacaa cattacctga atttacagtg
     6541 tctctacttg cttctattga tggtcatact actatgagtt taggaaatgc agttggttct
     6601 ataatatgta atacagggtt agcattaggg ttggttgttt ttataagtcc atttaatgta
     6661 gataaaaaaa tgtttttttc taagtcttta ctattaatag tatccgttat agttttaatt
     6721 ttattgagtt tagatggagt tataacaaga ggtgattcat tattacttat aataatatta
     6781 atcttttata tggttaataa ttatagaagt gtcgttggaa aatctgatac taaaaataga
     6841 aatattaaaa ataacactat taaagacata tctactaaaa aagggaaaaa ttatagaggt
     6901 ttttccgtat tagaaattgt aaaaatacta ttactttttg ctacaggatt gataatgatg
     6961 ataataggtt cacaaatcct tatagagagt ggagtaataa tagctagttt tttaaatata
     7021 cctcaaggta tagtaagttt aactataata gcacttggaa cttctctgcc agagatagtt
     7081 tcgtcgatta ctgctataag aaaaaatcat catgaaatat cagtaggaaa tattttgggt
     7141 gcaaacatat tgaatatagt atcagtaata gctgtatcag caataccaaa taacatacca
     7201 atactatctc aaaatagaca attggatata ccattcatga tattattatt attaatagta
     7261 ataataccaa cacttaaatc taataaactt agtagaatac aaggtatatt gatgttattc
     7321 acatattttc tgtacatatc aattttgtat ttcatgtata ttatttaact atactgttta
     7381 aaatattaat tgcagaatat cctaaaaaag tatataatgg ataggttaag attaattgat
     7441 aacggagagg aataatatat gaatataaca aagtttgaag atttaccaat aagtgaggga
     7501 ataaaaaaag ctatagctga aatggggttt gaagaaccat ctcctataca agcacagtct
     7561 atacctgcga ttttgtcagg taaagacgtt ataggacaag ctcaaactgg tacgggaaaa
     7621 acagctgctt ttagtatccc tatactagaa acaatagacc caaataatag aagtttacaa
     7681 gctgtagtgc tttgtccaac aagagaactg gcaatacaag tatcaacaga aataagaaag
     7741 ttagctaagt attcgcacgg aataaagaca ttacctatat atggtggtca gccaatagat
     7801 agacaaataa aatctctaaa gagtggtgtg caagttgtaa taggtacacc aggacgtaca
     7861 atagaccata tcaatcgtaa aacattaaag atggataatg taaaaatgat tatattagat
     7921 gaagcagatg aaatgctaga tatgggattt agagaagata tagaaatgat tttaagtaag
     7981 attccagaag aaagacaaac aactttcttt tcagctacta tgccaagagg aattttggaa
     8041 ttaactaaga gatatcaaaa agacccagag catataaaag ttgttagaaa agaacttaca
     8101 gtttcaaata caaagcaata ctatatagaa actaggtcat caaataaatt agaagtatta
     8161 tgtagattag tagatgtata tgaccctaaa ctatctgttg ttttctgtaa tactaagaga
     8221 aaagcagacg aattggtagg agatttacaa gcaagaggat attttgcaga tgcattacat
     8281 ggtgacttaa aacagactca aagagatata gtaatggata agtttagaaa tggtacaata
     8341 gatatacttg ttgctactga tgttgcagct agaggtatag atgttgatga tgttgaatgt
     8401 gtattcaact atgacttacc acaagatgaa gagtattacg ttcatagaat tggtagaact
     8461 ggtcgtgctg gtagagaagg tatgtcattt acttttgtat ttggaaaaga aatgagaaag
     8521 atgaaagata tagaaagata tactaaatct aaactaataa agcataatat accaacaata
     8581 actgatgttg aagagaaaaa agtaggtact ttctttgcac aagttaaaca aactatagaa
     8641 gaaggtcact taactaaaca attacaatgg ttagaaggtt tctgtaatga tgaagattat
     8701 gcaatggttg atatagctgc tgcacttgta aaactatctc taggtgaaga aatgaaagaa
     8761 gaaataatag aagaaaaacc tagaagagag cgtggagata gaaaaggtgg tactggagct
     8821 aaagatggta tgattagatt atttatcaat attggtagaa atcaaagagt acaagcaaaa
     8881 gatatagttg gtgccatagc tggagaagtt ggaataccag gtaaagtagt aggtactata
     8941 gatatatatg ataagtatac ttttgttgaa atacctaaaa aagatgcaaa aacagtaatt
     9001 gaaaagatga aagatattaa gataaaaggt aataagatta atatagaaaa agcaaataag
     9061 aagaaaaaat aacgtatagt atacaaaact ataattagaa aatatataaa ataatatatt
     9121 ggatatttaa aaagcctgat gtgttttcaa aacagatagt agataatctg taagagttaa
     9181 cagcataagg cttttcttgt tttatatgat gttataattt tccagattgt tgtgaaaaat
     9241 gtatattagt tccttttttt caaatctgat ttgaaaaaga gaatattaat tatctatata
     9301 gtttagatta taataaaaaa tgtaagataa caaaggggag aaaaagatgt caatgactta
     9361 tatggaccct agggtttttt tgattttaga aatggtgtta gaagaagaca caaatactct
     9421 agaacaattt tcaaatgttt tgggtgttag tactaggact atcagaaact atataaacca
     9481 gattaatcga gaattaaaag gtggtattgc taaaatagtt aaaaatgaaa aaggtacata
     9541 tagtataaga attgaggatg aaagtaaatt aattgagatt gtaaatttta atagaggaaa
     9601 gctatcaaat tttataaatt taaattcacc tgatgaaaga ataaattatg ttcttgatat
     9661 tttaactatg accaacaatc caattaccat agatgatttg gctgaagaga tatgtatagg
     9721 aagaacaaca ttaataaagg atttaaaaaa agcagacaga attttagctg aatatgattt
     9781 agaattaaaa aggaagccaa atataggtat gattttagtt ggtgaagaaa tggatataag
     9841 attattaatc cttgatagat tgtatgaaaa ttatattgat gtcttggaaa acgttaatca
     9901 gataaattta gttaaagatt atgatattga atgtcttaga gatgaattaa aaaagttatt
     9961 taaaaaagaa gaactctaca taacagaaca ggtcttgaga gatgttgaaa gatatataat
    10021 catgtctgtt ttaagaaatc taaatgggta caaatttgaa aagatagata agagatttga
    10081 agtgataaga tgttcagatg aatatacttt aggattaaag ctcaaagctt tattggaaaa
    10141 gatatttaat atagttttaa atgaaaaaga aaccatattt ttgacaatgc cattaatagg
    10201 tagaaaagct cctagtacaa aattagcact tagcagtata aagataaatg atagcgtaaa
    10261 ggaagttgta gatgaagtaa ctagtttttt attagagacc tcagggattg attttaaaga
    10321 agataaaaag cttatagaaa atttggaata tcacttgtat tttgcactta atagaatgag
    10381 attcaacata agggtaaaaa atcctctttt acaagaaata aaggaaagat atacatttcc
    10441 atatagcgtt gcaaagatag ctgcaatgat aattgaggaa aagtttgact tgaaaatatg
    10501 tgaccacgaa ataggatata tagcattaca ttttggaagt tattttgaaa aaaattcaag
    10561 aaaagttcta aatgtaaata gggtagctgt tgtttgtgga acaggtcttg ggactgcaca
    10621 acttattaga ataaagatag gaaaaatact tggagaaagt gtagaagtaa atactttttc
    10681 tgatatagaa gctaaagcta atttagattt attaaagtat tatgatttag tatttacaac
    10741 agtagatttc aaaacagact tagatgttcc aatttttaga gtaaatgcgt tatttaatga
    10801 agaaagtata aagaaagaga tagaaaatgc aatacttttg aaaaatagta taaatcttaa
    10861 gtctataaaa aagattgaaa aaaatatccc ttttataggt aaattaattg aagaggataa
    10921 gttctttata ttgaataaga gtacgtttat ggaaaattta gaagatatgt tagaaaaatt
    10981 agttcataaa ggaattattg atatagagtt caaaaaaaag gtattaaaaa gagaaaagaa
    11041 atcttcaaca gcattagata attatatagc attgcctcac tcagttaata ctaatgggga
    11101 aaatctttat ctggcatttg gaatattaga aaaaccagtg atttgggata caaaggaaat
    11161 tagaataata atattgatga taattccaga taagaatgta gatagtacag acctgataat
    11221 aaaaagttat gaagaagtac ttgaagtagg gagagataaa aagatggtag aagaacttgc
    11281 taaggtaaaa tcatttgatg aattttataa aataattacc aagaggaggt aataatatgg
    11341 acccagtaat ttttattata gcatcactta ttacagtgta tttattaaat atttttcttg
    11401 gatacttaca gcttaaagat tttaataaaa attatattga actaaaaaga aaaggaagag
    11461 tggcaattgg cagaaaaaaa ggaagaattt catcgggtac aattgtgttg atacttataa
    11521 atgatgatgg tgtgattgtc gagactagaa aaatgcaagg tgttacagta ttggcaagag
    11581 taaagtcatt tgaaggtttg gtaggaaaga gcttagagta tatagaaaaa tcagatttaa
    11641 ctgtttacaa taaactactt aaaaaagcaa tactagatgc agtcaaacag tacataacat
    11701 ttgagaaaaa taaatgtgaa gatacaaata atctgagtac tgatgaagag tgtaggatgg
    11761 aagtagttta aaaaaatata tttataagga ggagttaata tgacgcaatt ttttcaagta
    11821 ttagcaagtg gtgcagatag ttttatgaat ttatttaggg caggtgggca acaatttgta
    11881 ggttttgtaa cagatatagt accactttta gtagcattat tagttacaat gaatgcaatt
    11941 atcaacttta ttggtacaga tagagtcgag agacttgcaa aaaaatgttc tgcaaatata
    12001 tttactagat atttgatatt accagtcctt ggtacatttg tatttgcaaa cccaatgaca
    12061 ctatcattag gaagattctt gccagaaaag tttaaaccta gttactttgc agcagcaagc
    12121 ttttcatgcc atacaatgaa tggattattc ccacatatca atccaggtga actatttata
    12181 tatctaggaa ttgcaaatgg tataacaact ttgggattct caacagcaga tttagcagtt
    12241 agatatttgt tagtgggtat agttgcaaac tttattaaag gtgtagttac tgactataca
    12301 acaaaatttg tagagaaaca acaaggaata gcattaaatt cagatataaa gattggataa
    12361 ttactttaag gtataaataa atttttcaaa taaaaagata gtgtatagag gaggataaat
    12421 tatatggcat ttaacaaagt taaaatagta aaaggttctg gaggatgggg tggaccattg
    12481 ataatagaac caacagaaaa gaagaataag gtagtatatg tgactggggg agcaaagcca
    12541 gaaactgcag taaaaatagc tgaacttaca ggatgtgagt taatagatgg attttcacag
    12601 ggtgttaaag atgatgaaat cgcatgtgta ataataaatt gtggtggaac attgagatgt
    12661 ggtatatatc ctcaaaagaa aataccgact ataaatataa tgaaaacagg aaaaagtgga
    12721 ccattagcaa tgtttataaa agaagatata tatgtttcag gggtaaaacc aacaaatgta
    12781 gagcagttat aattgaggta gtaggaggga agaaatatgg cttatgatac taaaaagaag
    12841 ataagtgagc agagtcaagg aattgttgca aaaataggta tagcactagg aaatgtgaca
    12901 agtactcttt ttcaagctgg tagggatact atagatacat taatacatac tatactgcct
    12961 tttatggctt tcgttgcagt cctaataggt ataataaatc aatcgggtat aggaaattgg
    13021 gtagccaatt tacttactcc attaatcgga aatgttggag gtttagttgt cttatcaata
    13081 atatgttcat ttccgttgtt gtctccgttt cttggaccag gagctgttat agctcagata
    13141 gtgggagtat tggttggtac agaaatagga aaaggaaata ttccaccaag tttagcatta
    13201 ccagcattgt ttgcaataaa ctcacaagca gcttgtgact ttataccagt aggtcttggt
    13261 cttgcagaag ctgaacctga tacagttcaa gtaggagttc cgtcagtttt atattctaga
    13321 tttttaacag gtgcaccaac tgttctatta gcatggttag caagctttgg gttatataaa
    13381 tagaagaata ctttataaat aattagagag aggttcagtt gatatggaat taatatacag
    13441 tactcaaata aataaaatag gagataatgc aaatgaattt ttacaacata atatgtttgt
    13501 aactttcaaa gataatgctc ctcaagagtt agaaaattac tgttatatac attctgaaaa
    13561 taatttagta aaagaaatac ttacggatga tgtgctatca ataaataatg ttgattacaa
    13621 aattacttct gttggtgagt tagtaaacca aaatctttca gaattaggtc atataacatt
    13681 taagtttaca ggtgaaaaag atggagcaat tggaggaacc ttatatcttg aagaaaaaga
    13741 aatagcacct attggcgtag gtactatact aaaaataatt agaaaataaa atttaaaacg
    13801 aatctaatca tattataagt aaattataga taaaagtcaa tttaactaat caagttaact
    13861 tttaatgtga taaaaaaggg gaaatgaaaa tgagaaaagt ggctttagtc gttggaggag
    13921 gaagaagttt aggtgaatat ttatctaaac atttagctaa agtaggttat gatgttgcta
    13981 ttgcagattt aaatgaagac aacgcaaaaa aagtagctga agaaataaaa actgaatttg
    14041 gttgtaaatc tgtaggatat agttgtaatg ccatagatga aagacaggtt ataagtactg
    14101 tcaaagctat tgaaaatgat tttgatagaa tagatttatt agtgtacaat gcaggagtgg
    14161 ctgtaagtaa aaaaataact gattttggat acaaagattt taaatttatt gtagacgtaa
    14221 atttaaatgg gtactttcta tttgcaagag aagtatctaa agtcatgata gcaaaaggta
    14281 ttgagggtag aataatacag ataaattcaa aatcaggtag agtgggaagt aagcataatt
    14341 caggatattc agcaggtaaa tttgctggag taggacttac tcaaagttta gcgctagatt
    14401 tggcagaaca taagataact gtaaatgcac tgatgctcgg taatttactt gattctgata
    14461 tgtttgaaag tttaattcct caatatgcag taaaattggg aatacctgaa tcagaagtca
    14521 agcaagttta tatagataaa gtacctttaa aaagagggtg tagatttata gatgttgcaa
    14581 atatgcttac attttatgct tcaaaggaag ctgaatactg cactggtcaa tctataaata
    14641 ttactggtgg acaagttatg taaggtttta tggagtgtct caagatgaac tctttagttc
    14701 atgaggcact tttttgtata aaattaaata tattttcagc atttaaacaa tgaaaaagag
    14761 gtttatcttt attttgagat agcctcattt ttgtaattaa aaaacatagt gtttaagtaa
    14821 acatataaga ttgttagttt tttaaaagag aattttttaa taatattata tttatgaagt
    14881 atttttaaac ttaatt
//
LOCUS       DAGSRM010000096        14810 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.096, whole genome shotgun sequence.
ACCESSION   DAGSRM010000096 DAGSRM010000000
VERSION     DAGSRM010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14810
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.096"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            99..776
                     /locus_tag="MJE33_003018"
     CDS             99..776
                     /locus_tag="MJE33_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY9648938.1"
                     /translation="MYNLLVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVESI
                     VLDCIILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKP
                     YSLKELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEIL
                     LFLAENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWG
                     KGYKFVP"
     gene            848..2863
                     /locus_tag="MJE33_003019"
     CDS             848..2863
                     /locus_tag="MJE33_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBY9648939.1"
                     /translation="MKKYNILKGTLIVLIVSLLAITGLNLLYKYDNKYTYKSIRASNG
                     ILTIDNDTFTKGQLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSMRKGN
                     QSPYGNATYRMRIKNEGKDKLLSLELPEIFSASEVWINGEMVSKLGDVGTEKYRPKIK
                     NSVVSFIAKKNTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKIYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVEDLSY
                     FMVVLCIICINGSISKIEENKVYRFLILPLGITMSIISIVIPIILLPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAVTSNRFEP
                     IFTGWQTEYCGFVIVILFSVMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KRFLADVAHDLKAPVSAIQAFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNNFIKEIYENNLPDTQACGVNFEAYVLEKKIYAKINKEMLT
                     RAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKISDNGKGIPPENIPKVFDRFFTER
                     DNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLCFL"
     gene            2913..3593
                     /locus_tag="MJE33_003020"
     CDS             2913..3593
                     /locus_tag="MJE33_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY9648940.1"
                     /translation="MQTKILIIDGDKDNCQKLKGFLEEKGISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILSDGDGWTLCKKIRNVTTCPIVYMTYINEDQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
     gene            4110..4556
                     /locus_tag="MJE33_003021"
     CDS             4110..4556
                     /locus_tag="MJE33_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648941.1"
                     /translation="MAISDLAFGTECLKTSFQMLFSVISLVNINLLQAQILIAIGGLI
                     SILAMVSLTLFLSAKCKDSLTSMQIAFSICLIPMGIGVSWIGYLLPPGGIGFKISLLY
                     QMTSFNFLHLGGMSFWTPHIILFFSVIEIPIFIFLAIRTYCKHQVS"
     gene            4937..5854
                     /gene="pstC"
                     /locus_tag="MJE33_003022"
     CDS             4937..5854
                     /gene="pstC"
                     /locus_tag="MJE33_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBY9648942.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQSIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            5856..6680
                     /gene="pstA"
                     /locus_tag="MJE33_003023"
     CDS             5856..6680
                     /gene="pstA"
                     /locus_tag="MJE33_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBY9648943.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVVIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            6699..7463
                     /gene="pstB"
                     /locus_tag="MJE33_003024"
     CDS             6699..7463
                     /gene="pstB"
                     /locus_tag="MJE33_003024"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBY9648944.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            7491..8144
                     /gene="phoU"
                     /locus_tag="MJE33_003025"
     CDS             7491..8144
                     /gene="phoU"
                     /locus_tag="MJE33_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBY9648945.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            8302..9171
                     /locus_tag="MJE33_003026"
     CDS             8302..9171
                     /locus_tag="MJE33_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBY9648946.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGDYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            9258..10694
                     /locus_tag="MJE33_003027"
     CDS             9258..10694
                     /locus_tag="MJE33_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HBY9648947.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     DLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            10742..11569
                     /locus_tag="MJE33_003028"
     CDS             10742..11569
                     /locus_tag="MJE33_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBY9648948.1"
                     /translation="MKIKDNKAVFHVFIRIFLIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
     misc_binding    11860..12122
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            12198..>14810
                     /locus_tag="MJE33_003029"
     CDS             12198..>14810
                     /locus_tag="MJE33_003029"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBY9648949.1"
                     /translation="MENTNLSKTYNPKDFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKEHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPDKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPDKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDANAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGKDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLANQGFLAKAPE
                     SLIEEEKAKKEKFEE"
BASE COUNT         5851 a         1559 c         2717 g         4683 t
ORIGIN      
        1 ataataatac cagttatata tgaaaataaa acattataat ttgtaataac tataaaactt
       61 ggaattataa ttaaaatacc tttgaatgga gtggtgtaat gtataattta ctggttgtag
      121 atgatgatgt tgatatttta aacataaatc aaatttattt ttctaatata ggttttaatg
      181 tatatacatc agagactgct aaagatgcta tggatatagt agagagtata gttttagact
      241 gtataatttt ggatatttca ttgccagata tagatggata taaaatgtgc aatgatataa
      301 aagaaaaggt taacatacct ataatatttt tatctaatta tgagcatgaa gaagaaaaag
      361 ttcggggatt tttagctggg ggggatgatt atataacaaa accttatagt ttaaaagaac
      421 ttgaacttag aatatatgct cgtatgaggc agtacaaaaa tattacatca gttcctaatg
      481 tgttaaagtt ttcagcgtta gtaataaatg taaattcaag aaaagtaact tttgatggag
      541 aaagtataga tttaactact atggaatttg aaatattatt gtttctagct gagaacaaag
      601 aacaagtatt ttcaaagata gagatatata accaagtttg gaaaatgcct gatattggag
      661 atcaacatac agtgcaggtt catattgcac agatgagaaa aaaaattaat tctttaagca
      721 gggagcatca atacattcaa accgtatggg gaaaaggata taagtttgta ccataagtga
      781 gatactgtaa ttaataaata gttcttgttt tatttaatac gactatgaaa gagatgtggt
      841 tgatgcaatg aaaaagtata atatactaaa aggaactctc attgtattga tagtctctct
      901 gttggcaata acaggattaa atcttctgta taaatatgat aataaatata catataagtc
      961 tattagagca agtaatggaa ttttaactat agataatgat actttcacta aagggcaact
     1021 tgtctttttg atagatgact gggagtttta taatcataaa atatttaaac cgaatgattt
     1081 tacagagcaa gatataaaac cagaatatgt ttttataggt caatatccag atttttctat
     1141 gagaaaaggg aatcagtcac catatgggaa tgctacttac cgtatgagaa taaaaaatga
     1201 aggcaaggat aagctactaa gtttagagct tcctgagatt ttttccgcaa gtgaagtatg
     1261 gataaatgga gaaatggttt ctaaattagg agatgtagga acagaaaaat atagacccaa
     1321 aattaaaaat tcagtagtaa gttttattgc aaagaaaaat acagaaatta taatcaatgt
     1381 atcgaatttt tcacattatt atagtggttt gtattatccg cctgcattag gagaaacaaa
     1441 ggacataagt aatatgatgt tttatcgatt acttttttat agtataattt gcttcacaac
     1501 ttttgcaata gctatatttt cattgtctgt atggtttctt tcaaacaaaa gtaaaattta
     1561 cttatatttt ggatgtatgt gtgttttctt tgccatccat gtatcctatc cctttgtaca
     1621 tttgttagga ctgcctttag taagctttac atatgcggtt gaagatttgt catattttat
     1681 ggtagtttta tgtattattt gtataaatgg aagtatatcc aaaatagaag aaaataaagt
     1741 gtatagattt ttaattttac cattaggaat tacaatgagt atcatatcta tagtgatacc
     1801 aattattctt cttccatatg atatgtactt tgtcaacata tatgggaagt taatagacat
     1861 atataaatat attgtattta tatatatatt aatgtcttcg ctggtttcaa tatataaaaa
     1921 gacatgctct gagcacataa gttcttatat tttagtttca gtaaacactg tttttggaat
     1981 tagcatatta tttgatgcag ttacgagtaa tagatttgag cctatattta cagggtggca
     2041 aacagaatat tgtgggtttg taattgttat tttattttct gttatgatga taaacaggaa
     2101 tagaataata ataaaagaga atgaaaaact tacaaggaat cttgaacatg aagttgaagt
     2161 gagaactaat gaacttacaa cattgttaaa tgaacgtaaa agattcttgg cagatgtagc
     2221 acatgattta aaggcacctg tttcggctat acaagcattt atagatttga taaaggttgg
     2281 taatattcat gtagatgaag agacaagaac ctatctttat gctataaatc aaaagtcaaa
     2341 tgaagtacaa aatagagtac gtagtttgca agaatttaca tcacaagata aaagcattgg
     2401 tattagtgaa aaaatatgtc tgaataattt tattaaagag atatatgaaa ataatttgcc
     2461 agatacacaa gcatgtggtg taaactttga agcttacgta ttggagaaaa aaatatatgc
     2521 aaagataaat aaagagatgc ttacaagagc attcgagaat ttaatttata atgctttgac
     2581 ctttactcca tttgatggaa agataatttt atcaatggat aaagaagatg gatttgcaat
     2641 tattaaaatt tctgataatg gaaaaggtat acctccagaa aatataccaa aggtatttga
     2701 cagattcttt acagaaagag acaataatga gccaaaaggg caagggttag ggctatatat
     2761 tgtcaaatcc attataagag agcatggtgg agaaatattt gtagattcag aactaggaaa
     2821 aggaactgtt tttacaataa aattaagtct atgtttttta taataatagc ttatttaata
     2881 taaaagaata atgatatttg ggagtgatta taatgcaaac taagatattg ataatagatg
     2941 gagacaagga taattgccag aagctaaaag gatttctgga ggaaaaagga ataagtatag
     3001 atttagcata taattgtgaa gaagctattg gaaaaatatt tagcaataaa tatgatttaa
     3061 tatttttaga aataattcta agtgatggtg atggatggac tctttgtaaa aaaattagaa
     3121 atgtaactac ttgtccaatt gtttatatga cttatataaa tgaagaccaa agcattttaa
     3181 atgctttaaa ttctggtgga gacgattact taataaagcc tctaaattta gaaatattat
     3241 atgcaaaagt aaaagctatt ttaagaagaa tgaactcata tgtaaataat aatgaaaata
     3301 atacaaaatt agaggaatat aatagaacta gtggagtaat aaagttagaa gataaaataa
     3361 taaaattgac tccaacagaa aataagttgt taaacttttt tatagagaat cctgaaaaga
     3421 ctttgactac taaagagata tatgaacatg tatggatgaa tgaatactta gaggataact
     3481 atagtgtggt agttgcagtg aatggattaa gaaagaaaat agagaaagat tataaaaatc
     3541 ctcagaaaat tataacaata agaggagcag gatattattt taataaggaa taataaatat
     3601 attaatatca ttaataattg ttataaatgt gaaatgctaa cgatatctaa actaaattat
     3661 tataaaatag ctgactgtaa tttaattgat aaagcctatt cttctagact ttgccagtta
     3721 aattacagtc agttttatta tataaaaaaa tgcgtaacgg aactattatt ttatagatta
     3781 tatttgaaaa gataaataaa ttgtatatct aaatttatat attatttttt acctttaata
     3841 tatattaaat aataaaatag ttaaataata agattaagaa tttcttaata aatccagaag
     3901 atgctttcga ttatataata ttttatattt ttatattatt aattatatat gttgcaattg
     3961 ctgcacctac tttttctggt gagtatcaga cacaatcaga tagcatcctt cgttgtacga
     4021 aacatggaca tattcgtcta gcgtttacta aaatattagc tctattttct atatttataa
     4081 taatgtttgt tatatgtatt tctatccatt tggcaatttc tgatttagct tttggtactg
     4141 aatgtttgaa aacctcattt caaatgttgt tttctgtcat tagtctagta aatataaatt
     4201 tactacaagc acagattctt atagctattg gtggtctaat ttcaatactt gcaatggtta
     4261 gtttaacatt atttttatct gcaaaatgta aggattctct tacttctatg cagattgctt
     4321 tttctatttg cttaattcct atgggaatag gtgttagttg gattggatac ctattaccac
     4381 cagggggaat tggttttaag attagtttat tatatcaaat gacatcattt aatttcttac
     4441 acttaggagg tatgagtttt tggacaccac acattatact gtttttctca gtgatagaaa
     4501 tacctatttt tatatttttg gctattcgta cctattgcaa acatcaagta tcgtaacaat
     4561 ttgatggaaa taatagtgat ataaactaga ggatacaata attatatatg tagtataaaa
     4621 gttaaattta gttaacaaat cttaatagca gatataggta ataaattata cataaactgt
     4681 attattatct atatctgcta ttttaatata gagaaacaac agtatattgt aaagtaaatg
     4741 tcaaatatag ttttaaaagt aaaattaatg ttaaaaagta aatataattt tacacttgct
     4801 taacttaatc ctaatataga tttaacttaa aaaaagtaga ataaaagtat aggaaatttg
     4861 atgaagacga attataaaaa attaaatttt gaatacagaa cgaaaatcga taagtatatt
     4921 ttaatgggag ggtattatga tagtaaaatc tagattagta ggtttagaaa aaaatgacaa
     4981 taatggaaat aagacaaaat acgtaattga aaaagtagca aaaaatattt ttctacttag
     5041 tgctttagta gcagttgcaa gtttactttt gataatagga ttcgtatttt ataaaggatt
     5101 aagaccattt atatttgaag gatattcatt tatagacttt ttaactggtg cagattgggt
     5161 tccttctgcc aataaatttg gaatatcatc tatgatagta gcatcaatat ttgcaacact
     5221 tggagcactt gttataggag tacctgttgg tatattaacc tctgtattta tagcagaagt
     5281 agcacctaaa agattagcaa aaataatgtc accagcagtt gaacttttag caggtatacc
     5341 ttcagtatta tatggtgtat ttggtcttgc agtgatagtt ccaactatac aaagtatatt
     5401 taatcttcca aaaggtcaaa gtttgttagc aatagtaata gtattagcag taatgatgtt
     5461 accaacaata atatctgtat cagaaacagc aataagagca gtaccaaaag catacaaaga
     5521 aggttcatta gcacttggtg catcaaaaat agaaacaata ttcaaagttg tattacctgc
     5581 tgcaaaatct ggagtattag ccgcagtagt gttaggagta ggaagagctt taggtgaaac
     5641 aatggcagta atacttgttg caggcaactc accagttatg ccaactgcaa taacagatag
     5701 tgtaagacca cttacaacta atatagcact tgaaatggga tacgcatttg gtacacatca
     5761 agaaatgcta tttgcaactg gagtagtatt gtttacattt atattaattt taaacttagt
     5821 tttaaataaa ctttcaaata aggcggtaaa ttaagatgag aaagttcaaa gagaatctat
     5881 tgaagtcatt agtatatcta tcagcactat ttacaatagt atcattagta gttatagtgg
     5941 gatttatatt tataaaggga attggcaaca tgaattttaa tttcttattt agcaattatt
     6001 cagcatcagg tgatggtggt atacttccaa tgataattac aacattatat acagtagtag
     6061 tttctatagt tgtagcaact cctataggaa tattggctgc tatatattta caagaatatg
     6121 ctaaaaaagg aaaagcagtt acagctataa gatttgcaac tgaaagttta tcagggatac
     6181 catctatagt atatggatta tttggaggaa ttttctttgt agttacatta aaaatgggat
     6241 attccatagt tgcaggttca cttacagttg caataataat attaccagta ataattcgta
     6301 caacagaaga agctttaaaa acagttcctc aagcttatag agaatcatca ttggcattag
     6361 gagctacaaa atttcaaact ttatataagg tagtacttcc aagtgcaata ccaggaattt
     6421 tatctggagt aatcttatca gttggtcgta taattggaga atcagctgca atattactta
     6481 cagcaggtac tgtagccaag atgccaggtg gaatatttga tagtgcaaga acattaactg
     6541 tacactcata cttactgact aaagagagtg gagatatagc aacagcagcc agtataggaa
     6601 ttgttttaat agtaattgtg ttagcactaa acatgctagc aagatttgtt gctaagaaat
     6661 taaataaagc taattattaa atttaagagg tgtgaataat ggaattaatc gataagataa
     6721 aaatgagtgt aaaagatttg gatttatttt atggtgataa gcaagcatta aagaaaataa
     6781 atatggatat aaaagagaat aaggttactg ctttaatagg tccatctgga tgcggaaaat
     6841 caacttttat aagaacgtta aatagaatga atgacttaat cgaagatgta acaattaaag
     6901 gaaacatttc tgttgatgga gaggacatat atacctcaga tgatgttata aatcttcgta
     6961 ctaaagtagg aatggtattt caaaagccaa atccatttcc tatgagtata tatgataatg
     7021 tggcatatgg tcctcgtaca catggactaa gagataagaa gcaattagat aaaattgtag
     7081 aagaaagctt aaaaggtgca gctatttggg atgaggttaa agacaggctt aaatcatctg
     7141 cactaggact ttctggtggt caacaacagc gtatatgtat agcaagagca attgctatga
     7201 gaccagaagt tattcttatg gatgaaccta catctgcttt agacccaatt tcaacattaa
     7261 aagtagagga acttatagag gatttaaaaa aagactacac aatagtcata gttacacata
     7321 atatgcaaca agctgctcgt atatcagatg aaacagcttt tttcttaaat ggagaggtta
     7381 tagaatttag cgatacaaaa actatgttta ccactcctgt agataaaaga acagaagact
     7441 atataacagg tagatttggt taaaatatat aactataaag gagttagaca atggtcaata
     7501 atagcttaga tataagtatt gatactttaa aagaacaaac agtgcttatg atggaaaaat
     7561 gtgaagaagc aatagaaaag tctgtgactt gtatgataga gaaggattta gagggtgcaa
     7621 gaaaagtagt agaagatgat gaagaaataa ataaattaag agaagaaata agagataaga
     7681 gtatagagct aatagtatta aagcaaccaa tggcaaggga cttaagattt atttattcat
     7741 taggtaatat atctatagag ctggaaagaa taggagatta tgcagcaaat atagctagag
     7801 aaatgttgaa gataggtgga gaggcttata taaaagagct aatagatatt cctaagatgg
     7861 caaaagaatg taagtctatg atgtctcaga ctagagaagc tttaatctta tttgatgcaa
     7921 gtatagctta tgatgttgga actaaagatg atattgtaga tggattgtat gaacaagtac
     7981 aaattgattg tttaaaaata atgcatgaaa actcaaatac catagaccaa ggggtaaaac
     8041 ttctacttat aggaagatac cttgaaagaa ttggagacca tataacaaat gtatgtgaga
     8101 agataatttt tgcaattgag ggtaaaatgg ttgagatagg ataaaaaact gctttaaagc
     8161 agttttttta ttgtctaaat taagaaagaa ttgataaaaa aatatgatta atgggtaaat
     8221 ttaaaaaatt gtgatatact aggaacatat taaatatgag gatgcgtatt ttatataagg
     8281 tgctttttag gaggtggaac tttgaaactt caaaagatgg gtatgaggac tataaaaaca
     8341 ggaattagtg tcactttatg tatgattctt ggtgactatt tagtagagaa tatgttttat
     8401 tctctggtag catgtataat ttctgttcaa gatacagtaa aaggttcttt aaagacggga
     8461 atcaatagag tggaaggtac tattttaggt ggtataatag gattcatatt tgccatcatt
     8521 aaagctggag acccattttt atgtggtcta ggagtaatgt gctcaatata tttatgcaat
     8581 ctgtttaaaa taaagtcagt agtagttgcc tgtgtaacct ttctggctat acatctaggt
     8641 gtaggaaata gtgaccctgt atattattct ttacataggg tgttagatac atctgtgggt
     8701 gttttgtttg gagtacttgt taattattat gttgctagac caaattatct aaatagtgtt
     8761 atagatgagt ttaaaaagat agaagaactt tcaatgttgc ttattgaaga taaaattctt
     8821 agaaaagaaa atttaaatgt agagaaattt gaagaagaag tagaaaaatt agaagaaata
     8881 tattcaaagc ttattgatga gttggattat agtttgagtg aaactaatat agaaaaaatt
     8941 gagaatgcct taaggatatg tagagaaata cattttcata tgcagtctat agagttactt
     9001 gaaaataaac tttatttgaa agagcataca tataaatacc ttaagaaaac atataatgtg
     9061 gataagctag actggaatat agatgaagaa aaaagccctg tattcaacta tcatctaatg
     9121 aaaataatga ttgaaataga gttgttgaga aagctaaata aatcatatta ataatagtag
     9181 ttaaataata aataaaaaat agtatctttg taaatattaa acatatataa gtttaattta
     9241 tggaggtact attttttatg aatactagaa atgacaattc taaaatacta gaaatatata
     9301 aaatgaagct ttttggcgaa cttgtaaaag cagtgtggaa tgatgatata gaggatattg
     9361 aaaacttacc taataaaatt ttaaatgaag atttaaagga taatttaaat aattatttta
     9421 gtggaaaatt tgataaagat actatggtaa atttaattag ggtaactatg ggattggatg
     9481 tgacagaaga tacagataaa aatttaaaag agtcactaca tgaagctgta aatttagatg
     9541 aagtcaaaag accaataata tctgtaatat cagatgcttg caaatattgt gatgaaaata
     9601 ataaggaaga tgagtcatgt aagataagaa gtaagcatat taattgtaat gaggaagatg
     9661 tatgtagtgc ttgtggtgat tgtgtatcaa gatgtaagtt gggtgcaatt tcagataaaa
     9721 tagaatttat accaatgata aaattgttaa aagatataaa gagccctgta tatgcaatag
     9781 ttgcaccagc ttttgtagga caatttggga aagatgtaag ccctggtaaa ttaagaactg
     9841 cattaaaaaa gataggattt gaagatatga tagaagttgc tttggcagca gatatgctta
     9901 ctgcaaaaga agcatatgat tattatgagc atatgaaaaa ggacgaagaa ggatgcttta
     9961 taactagttg ttgctgtcct atttgggtta gtttgataca aaatagtttt cctcagatat
    10021 tggaaaatgt atcaccatcc gtttctccta tgattgcttg tgggagagct gtaaaaattt
    10081 taaatccaga tgctaaagtt gtattcatag gtccttgtat ggcaaaaaag aaagaggcaa
    10141 tgttagaaga tataaaagat gcagttgatt ttgtgcttac atttaaagag ttgcaagaaa
    10201 tatttgatgc attagagtta aatccagcaa taatggatga gagtaataga gtagaggcgt
    10261 ccctttcagg tagagtttat gctagaacag gaggtgtaag taaagcagtt aagttatcag
    10321 ttagaagaat tgacaaaaat ataaaatttg aagaaaaggc atttgaagga acaaaagagt
    10381 gcatggacgg tctaaagaaa gttctaaata aagaaataga agctacattt atagaaggaa
    10441 tggggtgtgt aggaggctgt gtaggaggtc cgaagcgtat tttaagtgtg gaagaaggta
    10501 ctaagaatgt tgatgaatat agtgaaagta cgaatatgga aactccattt gataatttaa
    10561 atgtattaca atttttaact tctttgggaa tcaaaagagt agaatcttta ggagaaaaag
    10621 aagaagaaaa ggttaaaaaa atatttagta gagatataaa aagtaataag aaagagaata
    10681 aatataaaaa ataacttttt aagtcttaaa cttatgcttt tataaaaggg agatatgggg
    10741 gatgaaaatt aaagataata aggctgtttt tcatgtattt ataaggatat ttcttatagc
    10801 tgttttaatt ttaactggaa cattattgga ggaaaaaatt aataatgttc cagttgaaaa
    10861 tataagttca aaaggtgaaa atacactgga actgctttct caaagtaata ctttaggaga
    10921 aatcatatac aaagcatctc aagaagaaac aaaaggtcca ggggctactt ctgaaaaagt
    10981 agcatacata acaatagatg atggtccatc aaaatataca aatgcactac ttgatatttt
    11041 aaaagaaaat gatgtcaaag cgacattttt catgcttaat cagaatatga aaaaccacaa
    11101 ggaagaagta aggagagtct tggaagaaga aaactcaata ggttttcata gtgtaagtca
    11161 tgatatacat aagttatata aaagcccaga agtaacagtt ggggaattta atacatgcaa
    11221 taatactcta caagaaataa ctggtcagag ttcaaaatta ataagattac catacggaag
    11281 taaaccatat atgccagaag gttcatataa taaacttatg gcaaatgatt atttaatttg
    11341 ggactggaat ttggatacac aggattggaa gtcttctaca agtcagatag taagtaatat
    11401 attatattat ggaagaaaaa gagatgaatt agtagtatta attcatgaga aagagcaaag
    11461 tttaaatgct ttaaataata ttataagaat cttaaaagag agaggatata caatattgcc
    11521 aattacagaa aatataacac ctaaaaattt ctggcaagaa aacttataat aaatttaaat
    11581 atagtaaaag ataaagtaaa ttatttataa aataaaacat ataatataaa aaatacgaat
    11641 aagaggtata ctgtaaggat aatataaaat taatgagaaa gcattaaaat taaaataatc
    11701 ttgcaattat tgacattata agacagaata actgtaaaaa catattttaa aatataacaa
    11761 ctatataaaa gagaatagac ataattaaga aaattcagaa aaaataaaat aaaaataatt
    11821 gacaaagaga ataatatttg tatataataa tatgtaatat taaagactga gaagaagagg
    11881 agtaagtaaa tactactaaa agagaggaaa attcactggc tgtaagattt tctaagtata
    11941 agatttactg aatgtagctt tggagtttct aaactgaact ataagtaagt ttagacggtt
    12001 agaaccgtta aattctttga gagtcaaagt tgataaaata acattaacca ataatttaat
    12061 tatatgttaa tgtattttaa gctttggaat taaggtggta acgcgagctt ttcgtccttt
    12121 ttaaagagga tgaagagctc ttttttattt ctaaagatat ttaaaaaatt tatttataaa
    12181 atacaggagg caggacaatg gaaaatacga atttatcaaa aacttataac ccaaaagatt
    12241 ttgaggctag gctatataaa aaatggatgg atgaagggta ttttaaatct aaaccaaatc
    12301 cagataaaaa accatttaca ataatgatgc caccaccaaa tataacaggg caattgcata
    12361 tgggacacgc tcttgaccat acacttcaag atatattaat cagatggaaa cgtatggatg
    12421 gatatgaagc tttttggctt ccaggaactg accatgcttc tatagcaaca gaagttaaag
    12481 ttgttgaaag aataaaaaaa caagaaggca agacaaagta tgaaataggt agagaagaat
    12541 ttttaaagag agcctgggaa tggaaagatg aatttggagg aaaaatatct aatcagctta
    12601 aacaactagg tgattcttgt gattgggata aagaaagatt tactatggat gaagggtgta
    12661 atgaagcagt tatagaattt tttgtaagcc tatatgaaaa aggtcacata tacagaggaa
    12721 atagaataat aaactggtgc ccagattgca aaactacttt atcagatgca gaagttgaac
    12781 atgaagaaca cgacggaaac ttttatcata taaagtaccc actaaaagat agtgaagact
    12841 ttttagaaat agctacaact agacctgaga caatgatagg agatactggt atagcagtaa
    12901 atccagaaga tgatagatat aaacatttaa taggaaaaac agcaatactt ccattagtag
    12961 gaagagaact tccaatagtt gcagatagtt atgttgattt agagtttgga actggtgcag
    13021 ttaagatgac tccagcacat gaccctaatg attttgaagt aggattaaga cataatcttg
    13081 agcagttaaa tactatgaat gaagatggaa ctatgaatga agtttgtgga aaatatgaag
    13141 gtatggatag atttgagtgt agaaaagcta tcgtagcaga cttaaaagaa caaggttacc
    13201 taattaagat aaaagagcat aatcataatg taggaacttg ttatagatgc catacagtag
    13261 ttgagcctag attatcagag caatggtttg ttaagatgga agaacttgca aaaccagcta
    13321 tagatattct taaaaaagga gaattagaat ttgttccaga taaatttgat aaaacttatt
    13381 tacaatggct agaaaatata agagattggt gtatatcaag acaattatgg tggggtcatc
    13441 aaataccagc ttattattgt caagagtgtg gagaaatagt agttgctaga aaaatgccag
    13501 ataagtgtcc taaatgtgga agcactcatt ttaaacaaga tgaagatgcc ttagatactt
    13561 ggttcagttc tgcattatgg cctttctcta ctctaggatg gccaaataag actgaagctt
    13621 tagattatta ttatccaaca agtgtacttg ttacaggata cgatataata ttcttctggg
    13681 tagttagaat ggcatttgca ggtatgttct gtatgaatga aaagccattt gaccatgttt
    13741 tggttcatgg attagtaaga gattctcaag gtagaaaaat gagtaaatct ttaggaaatg
    13801 gtatcgaccc attggagata atcgagcaat atggagctga tgccctaaga tttacattaa
    13861 caactggaaa ttccccagga aatgatatga gattctatat ggaaagagta gaatttgcta
    13921 gaaactttgc aaataaattg tggaatgcat caagatttgt atttatgaat attgatgaag
    13981 atataattaa aaacatgact agagaaagtg ttaaagagga cttaactcta gctgataaat
    14041 ggataatatc aagagcaaat aatattgtaa aagaagcaac taataacatg gataagtttg
    14101 atttaggaat agcacttcaa aagatatacg attttacttg gtcagaatac tgtgactggt
    14161 atattgaaat ggttaaacct agattatatg gggaagatgc aaatgctaaa tctgcagcat
    14221 tatatacatt aacttatgtt cttgaaaaaa tattaaaatt attacatcca tacatgccat
    14281 ttataacaga ggaaatatat actcatcttc ctactgttga aggatgtata atagtatctg
    14341 aatggccaaa atataatgaa gaagataaca tggctgaaga agaagatatg atgaacttat
    14401 tgatggaagg tataagaagt ataagaaatg tgagagcaga aatgaatgta ccaccttcta
    14461 agaaagctaa attgataata atcccaagtg aagaaaaaat agaagctatt gagcttggta
    14521 aagactattt tataacttta gcatctgctt caaatgtaga gatagcaaaa gataaatcta
    14581 atgttccaga agatgcagtt ggagtagtta ttgatggtgt tgaaatattt atacctctta
    14641 atgaattagt tgattttgaa aaagaaattg agagattatc taaagaaaag aaaaagttag
    14701 aaggcgaaat aaagagagtt aatggaaagc ttgcaaatca aggtttctta gcaaaagctc
    14761 ctgaaagttt aattgaagaa gaaaaagcaa aaaaagaaaa atttgaagag
//
LOCUS       DAGSRM010000097        14607 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.097, whole genome shotgun sequence.
ACCESSION   DAGSRM010000097 DAGSRM010000000
VERSION     DAGSRM010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14607
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.097"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            407..874
                     /locus_tag="MJE33_003030"
     CDS             407..874
                     /locus_tag="MJE33_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HBY9648950.1"
                     /translation="MKFLNVLVVVEDIEKSKKFYYDVLGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKSDSEVKFNGNDAELYFEEDNFDTFVERLSTMKDIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEVGETMSSVCRRFLDSGLSIDEVAKRMDVTVEYIESVLE"
     gene            2005..2667
                     /locus_tag="MJE33_003031"
     CDS             2005..2667
                     /locus_tag="MJE33_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9648951.1"
                     /translation="MSILEIKNLNKIYGKSETEVKALSQINLKINSGEFVVIVGKSGS
                     GKSTLLHIMAGLETPTNGDVIIDNINISSLDEKKRSALRKEKLGLIFQSYNLIPVLTV
                     EENIKLPTINIKNKDEAYINELMELLGIKDRRMYLPNQLSGGQQQRVAIARALVNKPS
                     IVFADEPTGNLDTKTESDVLSLLKESQKKYNQTIIMITHNIDITKYADRVIEIEDGFI
                     RE"
     gene            2676..5183
                     /locus_tag="MJE33_003032"
     CDS             2676..5183
                     /locus_tag="MJE33_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9648952.1"
                     /translation="MKLNYIVKKYIMGNKKNTLLIVVSIVISTALFLIMNIISEDATN
                     LMINQAKNEFTLKHAQYINPKDEEIKYIENNTSIDKVGKSMLLGISDIGKGQTLQILS
                     QDKVAEELNDIYTLEKGKLPVAENEIAIDSWYIKQKKIKNPIGKRITLDYRRQGIDQE
                     DLYTGEKEFKITGILNSNPILKAQGTSIGLISNDCAIKNIPTKNKYDQVMFTFKKEKN
                     IQRQSQKLIKNGNLNENNIYFNNELLIAISDSMNLKIPYIIVNIVLALATILLIYNIF
                     YILVSNRIKDFGIFRALGFNPNDIFKIMILEVSIYSIISISIGLILGGIIASLSREYV
                     IGVIYNVNYINSIKNQNYINTYIISILLSLGTIIISVSKPLMLSVKTDPMICIRRNNE
                     KVDIKQNSFINKFMIKLFKDYGNIASKNIQRNKKRTNLSIASMVIIFFLMATIYTKST
                     SNFLSDGVLKYWIQGDYLLHNIDISTIRSNNKGYDKNVLQEIKAIDGVTKVNSYRHKW
                     FNIKIDDKSINKNSDYWKKNKDNIEARSEVKDGIKVYNNSFEFLGIEDTDILEDFLIN
                     GKENIDKFSKQPYLYITKKSSDSLNIKKGDRIKVYFDIIDSKTNNFTKTISQEFIVCG
                     IITTLPVTSQVGAEFGGVISTNQFNKFTGISSYERFDIWTSKLANDKYVESELNKITE
                     KTNKGILIPYKAETAEYEKIDNQKTLIMVLVTGIIVILSLFNCCNTIVTSINSRSREF
                     ALFRGIGISKHEINKIVKLESYIYIIVSFCISIIPILIVRSIIIKPFESINLINWKFI
                     GAIILIVFILVSIIMITTLKTLNKIQNENFMEEIKTL"
     gene            5514..5858
                     /locus_tag="MJE33_003033"
     CDS             5514..5858
                     /locus_tag="MJE33_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648953.1"
                     /translation="MYCLSVVLAIYGIYVFINCTEYIYTMLSTKHLTVKGNEYTIINY
                     YMSNFSQYIIFAILLASMGFVIQKIVHMHFSEIELSGNYSTTVRNISDISLDNDNEMF
                     NRGNIENSILKK"
     gene            6096..6782
                     /locus_tag="MJE33_003034"
     CDS             6096..6782
                     /locus_tag="MJE33_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY9648954.1"
                     /translation="MSSILLVEDDLSLIYGLEYSIQKNGFSVDVARTVEEALQIYKEK
                     NYDLLLLDVSLPDGDGFDICKRVREASNVPIIFLTASDEEVNVVMGLDMGGDDYITKP
                     FKLNELISRIKALLRRYNFTSENVTELKSNNITVKLLENRVFKNEIELELTTAEYKLL
                     CLLMKNKNIVLTRKNILDKLWDGNGSFVDDNTLSVYVRRLRNKIEDNPENPKILLTVR
                     RMGYKWNVIK"
     gene            6793..8034
                     /locus_tag="MJE33_003035"
     CDS             6793..8034
                     /locus_tag="MJE33_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY9648955.1"
                     /translation="MKVFSNKDIKIFFLVIISVLLMFIVVGQIVVINITNDYKSALLE
                     HDYNIAGYLNSMGVDKSKIPAVFTTNEDKYTLKGKEILNTARYDFGTDDSFMPSVKLF
                     QGKYIKTMFLYLFVFFTMIIFILYVYFFKQQNKIEEASYKIDSFMNGNINVRLNTYEE
                     GSLSKLFGSIDAMSTSLNTHILKEKQNKEFLKNTISDISHQLKTPLTALMMYNQILQE
                     ESHNSEIVENFVQKSQNELERIESLIQNLLKITKIDSKTIILNKASVNVKKLITKILY
                     SFETRAEKEGKSISIRGLENTNLFCDYEWISEALSNLIKNALDNTKENDKIMIEWVET
                     PITTIISVSDTGNGIHIEDIHHIFKRFYRSKFSKSNQGLGLGLPLVKSIIEQHNGTIT
                     VESNFLQGSTFTLSFLKLTNM"
     gene            8105..8788
                     /locus_tag="MJE33_003036"
     CDS             8105..8788
                     /locus_tag="MJE33_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9648956.1"
                     /translation="MDILVVNNLCKTYGNGYTKVEALKDVSFTVSKGEFVTVVGESGS
                     GKSTLLNLLGGLDTPTSGKIFVDGKEICSMKEEKQTIFRRRNIGFIFQSYNLIPELTV
                     EQNIIFPLLLDYKKPDTIYLDEVLTILGLKDRKSHLPNQLSGGQQQRVAIGRALITKP
                     SIILADEPTGNLDSKNSSDVISLLKVTAEKYNQTIIMITHNTNIASTADRILQVSDGM
                     LKDLGVNRE"
     gene            8785..11118
                     /locus_tag="MJE33_003037"
     CDS             8785..11118
                     /locus_tag="MJE33_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBY9648957.1"
                     /translation="MRSYLELVPQYAKVHKKKNRTTILCIVIAVCLVTAIFGLADMAI
                     QCQKVQFIKDDGNWHIVLKNLNEEAISEISSRVDIAHYGRGQVIDNGEFKSKEILIQG
                     LDEDLAKQLGLEIIKGRYPVVENETLIDVDAVKSFNLKVGDFIKIKLADGRNKNYKIV
                     GEYSSLSSLRASDKHGLMLSILGTQAIKKSETYDYYYITFKDKVNIRKATSDIKNTFN
                     LSDKQIGKNEKLLSIIGQGDSDYSKPLYLTAGGLFILVLIASIIMIYNSFNMGVIERI
                     KFFGLLRCLGASKLQVKKFVVLESLILSVKAIPLGLLLGCIVTIISSIFLKYVNTELF
                     GSMPILKVSFIGIVFGIIVGFLTVILSAIVPAKKASGVSPLSAIRGNLMFKEILTSGK
                     ASKIANAGSSKIDVSMGINHAFSRKKSFILMTSSFAISIVLFLGFSVFIDFMYQALKP
                     IKPEAAHISIVDADFKSVLTKDDINEISNIKGIKKVYSRMMTDVEATYNNKEATSQLI
                     SYEKNQFDWAKKYLVKGEINEDKLESENIGLVEEGHGFNIGDKIYIKNGNSKKEIEVA
                     GILSSISFDVKDKYIGNIITSENTFTSITDIKDYIIIDTQVDEDAPEDLIQNIRNVLD
                     DNLEVRDKRQGNLEAKNSFYTMAVFVYGFVFIIAIISMFNIINSMNISVTSRINYYGI
                     MRAIGMSNKQLRKMVIVESSTYAVSGCLLGSVLGLILHRYIFVSLVTLKFHIDWQIPF
                     DLLFIIVVTMIIITLLAVRKPIKKICELDIIEVVNAQ"
     gene            complement(11226..11788)
                     /locus_tag="MJE33_003038"
                     /pseudo
     CDS             complement(11226..11788)
                     /locus_tag="MJE33_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(11902..13251)
                     /locus_tag="MJE33_003039"
     CDS             complement(11902..13251)
                     /locus_tag="MJE33_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBY9648958.1"
                     /translation="MEKAKKLGLFSMILLGINSIIGSGIFLLPGKVYNLAGQNSMFIY
                     IFATLLVLSILLCFAEVGSMFDKNGGAYLYSKKAFGDFIGFEVGTMSWVIRIISWSTL
                     AVGFATALGSFWPESATEYKGYIAAILVTLLSINSLFGIKSTKIMNNVITIAKLVPLI
                     VFIIVGIFFIKFVNIVPSGNVVNSSMGPAIILVFYAFTGFESFIVASGEMENPKKNLP
                     VALITTIFICAIIYILIQIVCMGILGDRLFENSIPIADASSVFLGNYGKVFISVATLI
                     SIFGINIGSSIVTPKCGSSLAEEGSLPAFIGKTNKYGAPYVAIIISLICCIPLVLTGS
                     FEQLAVMSVIARFAQYIPTCLSVIVLRKRTDVKASFKIPFGPVIPMVAILGSLWLLQQ
                     AWAEDISKPITQNRVLIGLGAMLLIAPLYIFMKKNKEIEHKNKSEDSKLYISREKIQ"
     gene            complement(13288..14508)
                     /locus_tag="MJE33_003040"
     CDS             complement(13288..14508)
                     /locus_tag="MJE33_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBY9648959.1"
                     /translation="MQQALKLKYQTKELGKDFVIESCNSIKPWLINIRRELHKIPELA
                     LEENLTKQKVISYLKEIGIDYMEFTKHNGIMAYILKESADKTICIRADMDALPIEEEN
                     NIPYKSIHSGKMHACGHDAHTTMLLGACKVLHSIKDKLNVNVKFLFQPAEEGFGGAKF
                     LVEDGCLENPKVDYIFGLHVMPHIETGFIETKYDTLNASVDTIKISIKGKRAHGAYPE
                     NGIDAIVTASQIVTSLQTIISRNLEPNNAAVLTIGKIYGGDAHNVICEDVKLEGTLRT
                     LNSKTRNFMIDKIAKIVGDTASAFGCVGTLHVSDENYPAVINEKELVDTVISNTKELL
                     GEEKFILRPNPSLGGEDFSFYTEHCKGAFFHLGCKNEEKGLISPLHTSSFNIDEDCLP
                     IGVMMHVMNTLYFN"
BASE COUNT         5767 a         1609 c         2210 g         5021 t
ORIGIN      
        1 aaattagaag aactaaaaac tatattttta ttaattataa tatttgaata atctttttaa
       61 tcatataaaa tgcatatact tgtaatattt aaaggtgata ttaatattat tagtgtatat
      121 taatattccc ttgaaattaa aaaaacatag atatttcagt aatctaaaat agtctacaaa
      181 aataacagtt ttagcaaaat taataagaat acatatagat ttaaacctaa aagtatgaac
      241 atgtctgttt tttacaagat taaaatttat attatatgat aaaattaact taactatttc
      301 aataagactt ataatttttt tctttatact agaccttatt gaaaccttta tagtttaatt
      361 ctaattatat attataaata aagtatgtgt atattaagga ggtaatatga agtttttaaa
      421 tgttttagta gtagttgaag atattgagaa atcaaaaaag ttttattatg atgttttggg
      481 gttaaaggta atctgtgatt ttggagaaaa tgttgtatta gaaggtaata tatcattaca
      541 agaaaaaaag ctttggttag aatttattaa taagagtgac agtgaagtta agttcaatgg
      601 aaatgatgct gagttatatt ttgaggaaga taattttgat acttttgttg aaagattgag
      661 tacaatgaaa gatatagatt atgttcatct agctatagag cataggtggg gtcaaagagc
      721 aattagattt tatgatttag atgggcatat tattgaggtt ggagaaacta tgtcatctgt
      781 gtgtagacgt tttttagata gtggtctttc aatagatgag gtagctaaaa gaatggatgt
      841 aactgtcgaa tatattgaat cagttttaga atagatggat tatattaaag taaaattgag
      901 gtgaaggctg taagtaaact tgtgtgaaaa caaatttgct tacagttttt atttttctaa
      961 aataaagaat ataatacttg tttagcttta tttgagtttt taaaaatatt atgatatttc
     1021 ttatatattt atactaaagt ttaaatttag aaaaaatata taaaagatag ttatatttac
     1081 tattatcttg tataattata tatactattt tatataacta tgctaaaatt taattgaata
     1141 aattaaaaat ataatgaaag tttagtttta attcatttat ttaataaaca tttcaagtaa
     1201 aataatgttt aaatcagaaa tataaataaa tattattata atcgttatta aagaccaaaa
     1261 atcccacctt agaagtttgt atcatatgaa acctttatag attatgaaag caataatata
     1321 gtgtatgata tataaaataa gttataaaag tattttttta gatattcaaa aagacattgt
     1381 aagttatatt aataatatgg gtatcaacga attttggata taatgagttt ttcaacttaa
     1441 agttatgtaa taatgaatgt aaagaatatt taatgaaatc attttgttgg ttatgtctag
     1501 ctgaatacaa taatctttta gataaagatt ttatatttga agataggtgt gaaaaaatta
     1561 agaagatatt aacatcagca gtttagaggc aaagcatgta aagtaaagtc tgggtaacta
     1621 aaagtagtaa tttagattgt aactattgtt ataaatatga aaatgaggaa acaggaaata
     1681 taagttagga tgtagaaaat aggttactaa ataaaaagct taattatata atcaaagaaa
     1741 taggtatata ctgtacaatt tcatggtgaa gaacttttcc taaataaaaa ggttggtaaa
     1801 agagatataa gaaataaaat tcaattataa gatatggtat gataactaaa ggcactatat
     1861 ggagtgaata ggcggaagaa ttttttgaaa attataaaaa tagtttctgt tgctacatat
     1921 caataagtat agatagagat tgcaaaatcc atagcaaaaa tcgaatttat aagtgtaaat
     1981 aacatgtaag acaaggagta aattatgagt attttagaga taaaaaacct aaataaaata
     2041 tatggtaaat cggaaacaga ggtgaaagcg ttaagtcaaa taaatctaaa aataaatagt
     2101 ggagaatttg ttgtgatagt aggtaaaagt ggaagtggta aaagtacttt actacacata
     2161 atggcaggac ttgaaactcc aacaaatggt gatgtgatta ttgataatat aaatatatca
     2221 tctcttgatg aaaagaaaag atctgcatta cgtaaagaaa agttgggatt aatatttcaa
     2281 tcatataatt tgattccagt tttaactgtt gaagaaaata taaaactacc aactataaat
     2341 ataaaaaata aagatgaagc ttatattaat gaattgatgg agttactagg aataaaagat
     2401 agaagaatgt atttgccaaa tcaattatca ggtgggcaac aacaaagagt agcaatagca
     2461 agggctcttg taaataaacc atcaattgta tttgctgatg aacctactgg aaatttagac
     2521 acaaagacag agtcagatgt attaagttta ttaaaagaat ctcaaaaaaa atataatcag
     2581 acaataataa tgataacaca taacatagat ataacaaaat atgctgatag agttatagaa
     2641 atagaagatg gatttataag agagtaggta gatttatgaa acttaattat attgtaaaaa
     2701 aatatataat gggaaataag aaaaatacat tattaatagt tgtaagtatt gtaatttcaa
     2761 cagcgttgtt tttaattatg aatataataa gtgaagatgc gacaaactta atgataaatc
     2821 aagctaaaaa tgaatttaca ttaaaacatg cacaatatat caatccaaaa gacgaagaaa
     2881 taaaatatat agaaaataac acaagtatag ataaagttgg aaaatcaatg cttttaggaa
     2941 ttagtgatat tggtaaaggt caaacactac aaattctatc tcaggataaa gttgctgaag
     3001 aactaaatga tatttacaca ctagaaaaag gtaaattacc agtagctgaa aatgaaatcg
     3061 ctatagattc atggtatatt aaacaaaaaa agataaaaaa ccctatagga aaaaggataa
     3121 cattagatta tagaagacaa gggatagatc aagaagattt atatactggg gaaaaagaat
     3181 ttaaaataac tggtatctta aatagtaatc ctattttaaa agctcaagga acatcaattg
     3241 gccttataag taacgattgt gctatcaaga atattcctac taaaaataaa tatgatcaag
     3301 ttatgtttac ttttaaaaag gaaaaaaata tacagagaca atcacaaaaa cttattaaaa
     3361 atggaaattt gaatgagaat aacatttatt ttaataatga attattaata gctatatctg
     3421 atagtatgaa tttaaaaata ccttatataa tagttaatat agtactagca ctagcaacta
     3481 tattattaat ttataatatt ttttatatat tagtatcaaa tagaataaag gattttggga
     3541 tatttagggc tttagggttt aatccaaatg atatttttaa aataatgatt ttagaagtat
     3601 ctatatactc tattataagt atttctatag gtctaatttt aggtgggata atagctagtt
     3661 tatcaaggga gtatgttata ggagtaatat acaatgtcaa ctatataaat tctataaaaa
     3721 accagaacta tataaacaca tacataattt ctatattatt aagtttagga actataataa
     3781 tatcagtttc aaaacctctt atgttatcag ttaaaactga tccaatgatt tgtataagaa
     3841 gaaataatga aaaagtggat ataaaacaaa attcctttat aaataaattt atgataaaac
     3901 ttttcaaaga ctatggtaac atagcatcaa aaaatataca aagaaataag aaacgtacaa
     3961 atttatctat agcatcaatg gtaattatat ttttcttaat ggcaacaata tacacaaagt
     4021 ctactagtaa ctttttaagt gatggtgtat taaaatattg gatacaagga gattatttat
     4081 tacataatat tgatataagt actatacgtt caaataataa aggttatgat aaaaatgtac
     4141 tacaagagat aaaggcaatt gatggtgtaa caaaagtaaa ctcatatagg cataagtggt
     4201 ttaatataaa aattgatgat aaaagtataa ataagaactc tgattattgg aaaaaaaata
     4261 aagataatat tgaagctaga tcagaagtaa aagatggaat caaggtatat aataattctt
     4321 ttgaattttt aggaatagaa gatacagata tactagagga tttcttgata aatggaaagg
     4381 aaaatataga taaatttagt aaacaaccat atctgtatat aacaaagaaa tccagtgatt
     4441 cgcttaatat aaaaaaagga gatagaataa aagtatactt tgatattata gattcaaaaa
     4501 caaataattt taccaaaaca atatctcaag aatttatagt atgtggaatt ataactacat
     4561 taccagtaac ctcacaagtg ggagctgaat ttggcggagt aatatcaaca aatcaattta
     4621 ataagtttac aggaatatca tcttatgaaa gatttgacat atggacgagt aaactagcca
     4681 atgataaata tgttgaaagt gagttaaaca aaataacaga aaaaacaaat aaaggaattt
     4741 taattccata caaagctgaa acagctgaat atgaaaaaat tgataatcaa aaaacactaa
     4801 ttatggtatt agtaactgga attatagtta tattatcttt atttaactgt tgcaatacta
     4861 tagttactag tattaatagc agaagtaggg aatttgcact atttagagga ataggaatat
     4921 caaaacatga aataaataaa attgttaaat tggaaagtta tatatatatt atagtaagct
     4981 tttgtatatc tataatacct attttaattg taagaagtat aataataaaa ccttttgaaa
     5041 gtatcaattt aataaattgg aaatttatag gggctattat cctaatagta tttattttag
     5101 tatctataat catgataact acactaaaaa cattgaataa aattcaaaat gaaaatttta
     5161 tggaagaaat aaagacttta tagtgaacag cctataaatt aaagaggttt tatatgaaat
     5221 taattgcaat tatcgataat ggtgtagata ttgattttaa ataatttaga aataaaaata
     5281 aaataagtta tccaagttat atgtatggtg taataggggt acaatgtata gcaaatataa
     5341 atattggctt aataaaactc tactcttaag tctactgtcg ttttaacaca agactaataa
     5401 taagtgtaac ctaaaaatta ttgataatac aataaccttg tttatgataa ataacaaata
     5461 ttaagtgttg taaagatagt aagtttaaaa aaatatcaaa gatttcaatc acaatgtatt
     5521 gtttatctgt agtgttagct atttatggta tatatgtatt tataaattgt actgaatata
     5581 tatatactat gctatctaca aaacatttaa cagtcaaagg aaatgaatat accattataa
     5641 attattatat gagtaacttt agtcagtata taatatttgc tattttactt gcatcaatgg
     5701 gatttgtaat acaaaagata gtgcatatgc atttcagtga gatagaactt agtggtaact
     5761 attcaacaac tgttagaaat ataagtgata tctctttaga taatgataat gaaatgttta
     5821 atagaggcaa tattgaaaat agtattttaa aaaaataaat aatataaata tataaaatgt
     5881 gattgaataa agcttagaat acttagtgta ttttaagctt tttttattat taagatttca
     5941 aagtattaaa ttaactatat aaaatcatat aaaaataatt ttaaaatatt taaatcattg
     6001 atttcaaggt attgtatttt taaaagagta aaaatataat aagttaggta tataatatat
     6061 ataaagtaaa aaataaaaaa atggaagtga ttgagatgag tagtatattg ttggttgaag
     6121 atgatttaag tttgatatat ggattagaat attctataca aaagaatgga tttagtgttg
     6181 atgtagcaag aacagtagaa gaagcattgc aaatttataa agaaaaaaat tatgatttac
     6241 tgcttttgga tgtatcctta cctgatggtg atggttttga tatttgtaaa agggtaagag
     6301 aagcttctaa tgtaccaatc atatttttaa ctgcttctga tgaagaagta aatgtagtga
     6361 tgggacttga tatgggtgga gatgattaca taacaaaacc ttttaaatta aatgagttaa
     6421 tttcaagaat aaaagcttta cttagacgtt ataattttac aagtgaaaat gtaactgaac
     6481 taaaatcaaa taatattact gttaaattac ttgaaaatcg tgtttttaaa aatgaaattg
     6541 agcttgagct tactacagca gaatataaat tattatgctt acttatgaaa aataaaaata
     6601 ttgtacttac aagaaaaaat atattggata agttatggga tggaaatggt agttttgtag
     6661 atgataatac tctgtcagta tatgtaagaa ggttaagaaa taagattgaa gataatcctg
     6721 aaaatccaaa aattctatta acagttagaa gaatgggtta caaatggaat gttataaagt
     6781 gagggtgtta atatgaaggt attttctaat aaagacataa aaatattttt tttggtgatt
     6841 atatccgttt tattgatgtt tatagtagta ggtcaaatag tagttataaa tataactaat
     6901 gattataaaa gtgcactgtt agaacatgat tataatatag ctggatactt aaatagtatg
     6961 ggtgttgata aatcaaaaat accagcagta tttactacta atgaagataa atatacttta
     7021 aaaggaaaag aaatcttaaa tacagctaga tatgactttg gtacagatga tagttttatg
     7081 ccaagtgtga aattgtttca aggtaaatat ataaaaacta tgtttttgta tttatttgtg
     7141 ttctttacta tgattatttt tatattgtat gtgtattttt ttaagcaaca gaacaagata
     7201 gaagaagcta gttataaaat tgattcattt atgaatggaa atataaatgt acggttaaat
     7261 acttatgaag aaggtagttt atcaaagtta tttggttcaa tagatgctat gtcaacatct
     7321 ctaaacacac atatattgaa agaaaagcag aataaagaat ttcttaaaaa tacaatttca
     7381 gatatatcac atcaactaaa aactccactt acagcattga tgatgtataa tcaaatattg
     7441 caggaagaga gtcataatag tgaaattgtt gagaattttg tgcagaagag tcaaaatgaa
     7501 ttagagcgta tagaatcatt gatacaaaac ttgttaaaga ttacaaagat agattcaaag
     7561 acaataattc ttaacaaagc tagtgttaac gtaaaaaagt tgattactaa aattttatat
     7621 agttttgaaa ctagagcaga aaaagaagga aagagtatct ctataagagg tctagaaaat
     7681 acaaacttgt tttgcgatta tgaatggata tcagaagctt taagcaacct tataaaaaat
     7741 gcattagata atacaaaaga gaacgataaa ataatgatag aatgggttga aacacctata
     7801 acaactatta tttcagtgag tgataccgga aatggaattc atattgaaga tatacatcat
     7861 atatttaaaa gattttatag gagtaagttt tcaaaaagta atcaaggcct tggattaggg
     7921 ttgccactag ttaaatctat catagaacag cacaatggaa ccataactgt agaaagtaat
     7981 tttttacaag gaagtacatt tactctaagt tttttaaagc ttactaatat gtaagatagg
     8041 atacatctta cagtaaggtg tatgaattat tcttatatta atcatcagaa aggagataga
     8101 gcaaatggat attcttgtgg ttaataattt atgtaaaaca tatggaaatg gatatacaaa
     8161 agtagaggca ttaaaagatg tatcatttac agtttcaaag ggagagtttg ttactgtagt
     8221 tggagaatct ggctcaggta agagtacatt attaaatctt ttaggaggtc ttgatactcc
     8281 aacctcagga aaaatatttg ttgatggtaa agaaatatgt tcaatgaaag aagaaaaaca
     8341 gactattttt cgacgtcgta atataggttt tatctttcaa tcatataatc ttatacccga
     8401 attaactgtg gaacaaaata taatatttcc tttactacta gattacaaaa aaccagacac
     8461 tatttatcta gatgaagtat tgacaatttt aggtcttaaa gatagaaaaa gtcatcttcc
     8521 aaatcaatta tctggtggtc agcaacaaag ggttgcaatt ggaagagcat tgattacaaa
     8581 gccatcaatt atattggcag atgagccaac aggaaatctt gacagtaaaa atagcagtga
     8641 tgtaatttca cttttaaaag tgacagcaga aaaatataac caaactatta ttatgataac
     8701 tcataataca aatatagctt ctacagcaga taggatacta caggtttctg atggaatgct
     8761 taaagaccta ggggtgaata gagaatgaga agctatttag aactagtacc acaatatgca
     8821 aaggtacata agaaaaaaaa tagaactaca attttatgta ttgtcattgc agtatgttta
     8881 gttactgcta tttttggact agcagacatg gctatacaat gtcaaaaagt acaatttata
     8941 aaagatgatg gaaattggca catagtactt aaaaatctta atgaagaagc tatatcagaa
     9001 ataagtagta gagtagatat agctcactat ggtagaggac aagtcattga taatggagag
     9061 tttaaatcaa aggagatttt gatacaaggt ttagatgaag atttagcaaa acaattaggt
     9121 ctagaaataa taaaaggtcg ttatcctgtt gtggagaatg agactttaat agatgtagat
     9181 gcagttaaaa gctttaatct aaaagtagga gattttatca aaataaaatt agcagatggt
     9241 agaaataaaa attataagat agtaggtgaa tattcatcat tatcaagctt aagagcctca
     9301 gataagcatg gattaatgct ttcaatatta ggtacacaag ctatcaaaaa atcagaaaca
     9361 tatgattatt attatattac atttaaagac aaggttaata ttagaaaagc tacttctgat
     9421 attaagaata ccttcaattt aagtgataaa caaatcggta aaaatgaaaa attactttct
     9481 attataggtc aaggtgactc tgattattct aaaccacttt acttaacagc aggaggactt
     9541 tttatacttg tgttaatagc aagtattatc atgatatata atagctttaa tatgggtgta
     9601 atagagagaa taaagttttt tggactatta cgttgcttgg gagcatcaaa actccaagtc
     9661 aaaaaatttg ttgttttaga aagtttaatt ctaagtgtaa aggctattcc actaggattg
     9721 ttattaggat gtattgtaac cattatatcc tctatatttc tcaaatatgt gaatacagag
     9781 ttatttggtt cgatgccaat tttaaaggtt agctttattg gtattgtgtt tggaataata
     9841 gtaggatttt taacagtaat attatcagct attgttccag caaaaaaagc ttctggggtg
     9901 tctccactaa gtgctatacg tggtaatcta atgttcaaag aaattttaac tagtggaaaa
     9961 gcaagtaaaa ttgctaacgc aggaagttct aaaattgatg tttctatggg aataaatcat
    10021 gccttttcaa gaaaaaaatc ttttatactt atgacttctt catttgctat tagtattgta
    10081 ttgtttttag gttttagtgt atttatagac tttatgtatc aggcacttaa acctataaaa
    10141 ccagaagctg ctcatatatc tattgttgac gctgacttta agtctgttct tactaaagac
    10201 gatattaatg aaataagtaa tatcaaagga ataaaaaaag tatacagtag aatgatgacg
    10261 gatgtagagg ctacatacaa taataaggag gcaacatctc agctaatttc ttatgaaaaa
    10321 aatcaatttg actgggccaa aaagtatcta gtaaaagggg aaatcaatga agataaatta
    10381 gagtcagaaa atatcggatt ggtagaagaa ggtcatgggt ttaatattgg agataagata
    10441 tatattaaaa atggaaattc taagaaagag atagaggtag caggtatact ttcttcaatt
    10501 tcatttgatg ttaaagacaa atatattgga aatattataa catctgaaaa tacttttact
    10561 agtataacag atataaaaga ctatataata attgatactc aagttgatga agatgctcct
    10621 gaagatttaa tccaaaatat aagaaatgtt ctagatgata atttagaggt tcgagataag
    10681 cgacaaggta acttagaggc taaaaattca ttttacacta tggcagtttt tgtatatggg
    10741 tttgtattta taattgcgat tataagtatg tttaatatca taaacagtat gaatataagc
    10801 gttacaagta gaataaatta ctatggtatt atgcgtgcaa taggcatgtc caataagcaa
    10861 ttaagaaaaa tggtaatagt agaatcaagt acatatgctg taagtggctg tttattgggg
    10921 agtgtacttg gattaatatt acatagatat atatttgtat ccttagtaac attaaaattc
    10981 catatagact ggcaaatacc ttttgattta ctgtttatca ttgtagtaac tatgataata
    11041 ataactttgc ttgcagtaag aaaacccatt aaaaagattt gtgaattaga cataatagag
    11101 gtagtaaatg cacaataaat ataagtagta atttaaatag aggtagtaat ttaaataaaa
    11161 aatcgaagta aatatgaaat agaaaatatc tttcataatt tacttcgatt tttgtgttat
    11221 ataatttact ttagataagt tccatcttca tcttgataaa ttaagtcttt cttcataagt
    11281 actccaagag cacgtttaaa attttcttac ttgaattaaa taagatagaa atatctttag
    11341 gatcagattt atcattgaat ctcatatatc catctgaacc ttctaaatag cttaaaatct
    11401 tattttctaa ggtatcaagt tcatcttttc tgtttcctct aggagaaagt cctaatttac
    11461 catcatcgta tatttttata acatgtaaat tttcaagaac atccccagct tttaattctg
    11521 taaagtattc attatgaagg attacaccag catatttatt gtcaacacat atcatagcag
    11581 agttgttggt ttggaatcca tatacaactc catttacaac atctcctaca ttgtaagtat
    11641 ggtctgtttc taaatatgaa tctatgtctg tagtagcagc tattcttccg cttttatcca
    11701 agtatatata gaataaatat ttttcatctc taaacaatgg ataagtctta gctttaaaag
    11761 gaactagtat gtcttttgta agtcccatat ctataaaagt accaatatcc gtgttatcaa
    11821 ctacttttag atacgtaaca tctccatttc taattatttc tatagtatta gaaaaataat
    11881 ttttaaatag tttgtaaaat attattgaat tttttctctc gaaatatata atttagaatc
    11941 ttctgattta tttttatgct cgatttcctt attttttttc ataaatatat atagaggagc
    12001 tattaacaac attgccccta atcctattaa aactctattt tgagttatag gttttgaaat
    12061 atcttctgcc caagcttgtt gtaacaacca taagctacct aaaatagcta ccattggtat
    12121 aacaggacca aatggaatct taaaagaggc cttaacatct gttctttttc ttaaaactat
    12181 tactgataaa caagttggta tgtactgagc aaatcttgca attacactca ttactgcaag
    12241 ttgttcaaaa cttccagtta gcacaagtgg tatacaacaa attaaagata ttattattgc
    12301 tacatatgga gcaccatatt tatttgtttt tcctataaat gcaggaagag aaccttcttc
    12361 tgctagagaa gaaccacatt taggagttac tattgatgaa cctatattta taccaaatat
    12421 agatattaat gttgctacag atatgaaaac tttaccataa ttgcctaaaa atacacttga
    12481 agcatctgca attggaatac tgttttcaaa tagtctatct ccaagtatac ccatacagac
    12541 aatttgaatt aatatataaa ttattgcaca tataaatatt gtggttatta atgcgactgg
    12601 aagatttttt ttaggatttt ccatttctcc acttgccaca ataaatgatt caaagccggt
    12661 aaatgcgtaa aatactaata taatggctgg acccatacta gagttaacta catttcctga
    12721 aggaacaata tttacaaatt ttataaagaa tattccaact attataaaaa ctattaatgg
    12781 tactaattta gctatagtta ttacattatt cattatcttt gtgcttttaa ttccaaataa
    12841 gctatttatt gaaagtagtg ttacgagtat tgcagctata taacctttgt attcagtggc
    12901 agattctggc caaaatgaac ctaaagctgt tgcaaatcct actgctagag tagaccatga
    12961 tataattcta ataacccaag acattgtacc aacttcaaat cctataaaat ctccaaaagc
    13021 ttttttggag tatagatatg ctcctccatt cttatcaaac atgcttccaa cttctgcaaa
    13081 acataaaagt attgatagaa ctaagagtgt agcaaaaatg tatataaaca tactgttttg
    13141 acctgctaga ttataaactt tacctggtag taagaatata cctgaaccta ttatgctatt
    13201 aatccctaat aatatcatac taaatagacc taatttctta gctttttcca tattaattta
    13261 tctcctattc ttttattaat tactgtacta attaaaatac aaagtattca ttacatgcat
    13321 cataacacct attggtaaac aatcttcatc tatattaaag ctagaggtat gaagaggtga
    13381 aattaagccc ttttcctcat ttttacatcc taaatggaag aatgctcctt tacaatgttc
    13441 tgtgtaaaaa gaaaagtctt caccacctaa agacgggttt ggtctcaata taaatttttc
    13501 ctctccaagt aattctttag tatttgatat aactgtatct actaattctt tttcatttat
    13561 aacagcagga tagttttcat cacttacatg taaagtccca acacaaccaa atgcagatgc
    13621 agtgtctcca acaattttag ctattttatc aatcataaag tttcttgttt tgctatttaa
    13681 ggttcttaaa gtgccttcta gttttacatc ttcacatatt acattgtgtg catcaccacc
    13741 atatatttta cctatagtca aaacagcagc attatttggt tctaagtttc tacttattat
    13801 agtttgtaat gatgtaacaa tttgtgaagc tgtaactatt gcatcaatac cattttcagg
    13861 atatgcacca tgagctcttt tacctttgat acttattttt atagtgtcta cactagcatt
    13921 taatgtatca tattttgttt cgataaatcc tgtttcgata tgtggcataa catgaagtcc
    13981 gaatatataa tcgaccttag gattttctaa acatccatct tcaactaaga attttgctcc
    14041 tccaaaacct tcttctgctg gttgaaataa gaattttaca tttacattaa gtttatcttt
    14101 tattgaatgt aaaactttac atgctccaag tagcatggtt gtatgtgcat catggccaca
    14161 agcatgcatt tttccagaat gtattgattt ataaggtatg ttgttttctt cttcaatagg
    14221 aagagcatcc atatctgctc ttatacaaat cgttttatct gcactttctt ttaaaatata
    14281 tgccataatt ccattgtgtt ttgtaaattc catataatca attccgattt cttttaaata
    14341 agaaattacc ttctgtttag taagattttc ttcaagagct aattctggaa ttttatgtaa
    14401 ttctcttctt atgtttataa gccatggttt tatagaatta caactttcta ttacaaaatc
    14461 ttttcctaat tctttggttt gatattttaa tttcaaagcc tgttgcattt ttattcctcc
    14521 aattgctttt tattgtttta ttaatataat aagcaattat tatgccataa ttttcgatgg
    14581 agaactttgt tttttataag aatattg
//
LOCUS       DAGSRM010000098        14446 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.098, whole genome shotgun sequence.
ACCESSION   DAGSRM010000098 DAGSRM010000000
VERSION     DAGSRM010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14446
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.098"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(180..806)
                     /locus_tag="MJE33_003041"
     CDS             complement(180..806)
                     /locus_tag="MJE33_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBY9648960.1"
                     /translation="MKYEWRKRDKELYLPKKNPVIVEIPEQKFIMLKGSGDPNSKEFT
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTVFPLEGIWDKGEKSKGLDVLIKEDLIYT
                     IMIRQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQKLHIGSYDNEPES
                     FDIMNEFCKENKLIRKSYTHREIYISDARKVSPESLKTVLRFEVEREV"
     gene            complement(882..1853)
                     /gene="nrdF"
                     /locus_tag="MJE33_003042"
     CDS             complement(882..1853)
                     /gene="nrdF"
                     /locus_tag="MJE33_003042"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBY9648961.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQAFWEQNVKQFWLPEEISVSKD
                     IKVWNELSNKEKELYKKVLGGLTLLDTKQGNNGIPSMMSLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNLEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KMDKEMLEEKMYSILYSLMENEIEYTNVIYRESGLEKEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            complement(1866..3965)
                     /gene="nrdE"
                     /locus_tag="MJE33_003043"
     CDS             complement(1866..3965)
                     /gene="nrdE"
                     /locus_tag="MJE33_003043"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBY9648962.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDKEALSSYIEEFIQPKLKKFN
                     NLEERLKYLIDEGYYSKEVINQYSIDVIKNIYDMIDKHKFEFQSYMSANKFYQNYALK
                     SNDGKEILETYNDKVLIVALTLGNGNKDLALNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMDEWYDKLVNDSDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDVGIIKMSNLCCEIFQYQTPSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIERNQT
                     FEGFDKSEYVKGRNSKVLSKYYEQSYLPKSEKVRALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAVAPTQSISYVQNATSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNMLYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFVTDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            4443..5168
                     /locus_tag="MJE33_003044"
     CDS             4443..5168
                     /locus_tag="MJE33_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBY9648963.1"
                     /translation="MKTISKQELEKTLEKHYLWLKNNNAGEKADLKELRLENMDLRGY
                     CLSNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNADLSGASFRFCDLS
                     NSDIRGANLENSNLEHATLNGVISDESTRFFKLYCPETGAFIGYKKCFNFRMVQLLIP
                     SDAKRVSATSNACRCDKAKVLSITSIDGKESFKSARAYADENFIYRVGETIVAEDFNE
                     NRWKESTTGIHFFLTREEAIGYL"
     gene            complement(5296..6492)
                     /locus_tag="MJE33_003045"
     CDS             complement(5296..6492)
                     /locus_tag="MJE33_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9648964.1"
                     /translation="MLKTNNLSVGYNNKVVISNINVEVKNGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINCISRKALSKKMALVLTNRLLGDLMTVQDIVNIGRYP
                     YTGFFGSLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKKLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDEDIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFEPISDESFDLAIRNLNDSKIIIDT
                     GFSVGETNKRNIDIIKEALKLDKKVYSFRNRDESKKYYDSLDNKIEHIDKVSQIINSA
                     DINNLL"
     gene            complement(6495..7520)
                     /locus_tag="MJE33_003046"
     CDS             complement(6495..7520)
                     /locus_tag="MJE33_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBY9648965.1"
                     /translation="MDFINKMRIRNTTIFIVLIISMILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGACLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVKNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILIIVGILGLLMTVF
                     IIKPLNAFLLGEEYAKSIGINIRFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQIS
                     KLIFKTSDNRVLIPVVIFLGSIVTALCDLISRVILSPVELPISTITSLLGAPIVVMLL
                     VKRKDRA"
     gene            complement(7592..8722)
                     /locus_tag="MJE33_003047"
     CDS             complement(7592..8722)
                     /locus_tag="MJE33_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBY9648966.1"
                     /translation="MKFKSNVLKQFFLIAMVIFMVGGLIGCSKSDKENKQASTGKKLE
                     LKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLIAST
                     TQACSLRTIDELDSIKAVTTEEQYWTIKEIKDLMKDGKIHYVGSNLAPDYEKIVDLNP
                     DLTIVSSGLSEADTKFIDKLKELGLNYVVDNEYKEQNPFGRMEWTSLIAAFYDKEDMA
                     TSELNKTVQKVEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGINAC
                     ASIDSNEGRVSIEQLHEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKNGNL
                     WVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     gene            complement(9316..9711)
                     /gene="tnpA"
                     /locus_tag="MJE33_003048"
     CDS             complement(9316..9711)
                     /gene="tnpA"
                     /locus_tag="MJE33_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBY9648967.1"
                     /translation="MKLDSNNHSVFLLHYHLVMCIKYRRKVIDDSISDRLKDIFVDIA
                     PNYGVVLIEWNHYVDHVHLLFKAKPNTEISKLLNAYKSASSRRIKNEFPSIRKYLWKE
                     YFWSKSYCLVTTGGATLEDLEQYIENQEE"
     gene            complement(9876..10883)
                     /locus_tag="MJE33_003049"
     CDS             complement(9876..10883)
                     /locus_tag="MJE33_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003358391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBY9648968.1"
                     /translation="MRLREYIGDKVVFLGTNLIIFLIASGIMLYSKINVVLIFMLFCI
                     WFLPLFTYMLIDYLQYKKYFNSIDNILDALDKKYLLPEVIDDVNFVTGEKINDILKVI
                     SRNMHENIKYYNDNQEEYKEYIETWVHEIKTPIASSKLIIENNSNEITKKIDTQLDKV
                     ENFVEQVLYYSKIDNVNKDYIIKKLNLEEVVNEVIKRNYRDFINKNIKLELGEINKTV
                     YSDSKWVEFILNQIVVNSIKYIKSKDGLIKISAKRVENSIVLTIEDNGVGIEERDINK
                     VFEKGFTGENGRVFGKSTGIGLYLCKKLCNKLGLGLILESKVNVGTVIKVSFPIDYNL
                     F"
     gene            complement(10880..11551)
                     /locus_tag="MJE33_003050"
     CDS             complement(10880..11551)
                     /locus_tag="MJE33_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005210744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY9648969.1"
                     /translation="MEKIIIVEDEEVIRDELKSFLERYGYEVMAPTEFDNIVEFILSE
                     KPNLVLLDINLPIYDGYYICRVLRKESNIPIIIVTSRNTDMDELMSMNLGADDFVTKP
                     YKTQILLARIVALLKRSGLNSNVNNTLIFKGIELNLFNATLRYKDKEIELTKNEFKIL
                     SYLVENQGSIVSRDSFMEYLWHTNWFVDDRTLTVNITRLRKKLDEIGIENLIETRRGL
                     GYIIS"
     gene            complement(11628..13622)
                     /locus_tag="MJE33_003051"
     CDS             complement(11628..13622)
                     /locus_tag="MJE33_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003400181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9648970.1"
                     /translation="MYSKIAIKNVRKSFKDYSIYFLTLTLAICIFYSFNSIESQKALM
                     EMNASTKSHVEMLKNMISYMSVFVSVILCSLILYANNFLVKKRNKELGIYMTLGMEKV
                     KISKILVFETIIVGIVSLVSGLILGLLVSQGLSILLLKLFEFNMSEYNFVISISSIIK
                     TIVYFGIMFLLVMIFNTYIISKYKIIDLLTIGRKTEDIKFKSPFVYTITFILCIISLI
                     IAYSMVLKVGLNPKNPKFIMSIVLGVIGTILFFYSLAGFALYIVKKNKNIYFKGLNIF
                     VVKQINSKINTNFISISIICLMLFLTIGILSTGFGFKSALNAGLKGATPFDASATMIV
                     HEKCKVKNIKESFEKVGIKFNKGDKVAYYDEYINGNKLSDVMQGQYSGRVDFEVAYIK
                     SSQYNEIRKLRGEKSITLSKDEVLITSNATEVVPSIEEYMKNNKIIKIDNKAYNIKNK
                     KVIKDNLRTDFTETNALTVVVNDKLCDSMEISSSIVNINFASDNNEKSQENFDKRLKS
                     KYSSTSFYEVGTVSGGSRNAMYNDNNGMTSTILFVGIYLGIVFLISSMAVLALQQLSE
                     ASDSIDRYVSLKKLGASKNSISKTIFTQTLMYFSIPVGLALVHSVVGIQVANNFISRL
                     NKPDIGASSLMTVGILMLVYAVYFYTTYQGYKNIVIKKLN"
     gene            complement(13612..14379)
                     /locus_tag="MJE33_003052"
     CDS             complement(13612..14379)
                     /locus_tag="MJE33_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014521021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9648971.1"
                     /translation="MKNILSVEKIEKYYGNKDNITKAIDNISFKVDEGEFVGIMGPSG
                     SGKTTLLNCISTIDNVTIGKIIINNNDITRLKSKNLDKFRQNELGFIFQDFNLLDTLT
                     AYENIALALTIKGEKPSKIDSKIRDVATYLEIEKVIDKYPYQMSGGQKQRVASARAIV
                     TNPSLILADEPTGALDSKSARLLLERFESLNQDLKATILMVTHDAFTASYAHRILFIK
                     DGKIFTELVRGADSRKEFFNRIIEVTTLIGGDDNDVF"
BASE COUNT         4711 a         2473 c         1472 g         5790 t
ORIGIN      
        1 ttatataata atacttatat aattagtatt attatttaat agcaacagtt attcacacta
       61 ataaaagtag aaagttttta aaatattatg aaacatcaaa tttaatttta aaattgcatc
      121 aaaaaatgaa gtctcaacag acttcacttt ttataactct tataaattat tcaaaatatt
      181 tatacttccc tttctacttc aaaacgtaaa accgtcttca atgattcagg cgatactttt
      241 ctagcatcac ttatataaat ttcacgatgt gtgtaagatt ttcttataag tttattttct
      301 ttgcaaaatt cgttcataat gtcaaagctt tctggttcat tatcatatga acctatatga
      361 agcttttgaa cacatagccc ttcattaata gacccaaatc taactttttc aagaagatta
      421 tgaggttttt tcttctttac tatatcagta gctttatcca acaggtcttg tgtaacaaaa
      481 tctggctgac gtatcattat agtgtaaatt aaatcttctt taataagtac atctaagcct
      541 ttactttttt ctcccttatc ccatatacct tctaaaggaa aaaccgtata gtcaaaatac
      601 ccttctggta caattccttt tttaggcatg gtttttattg catatgccaa agaataaagt
      661 actcctattg cttcagtaaa ttctttagaa tttgggtctc cactcccttt taacatgata
      721 aatttctgtt ctggtatttc aactattaca ggattctttt taggtaaata taactcttta
      781 tctctttttc tccattcata tttcataaaa atacctccat atatatttta tacattgata
      841 ttatcacgct aaaagtgaca acagtgtgtc ataattataa attatattat aaaatcttca
      901 tcctctaatt cctcatacac tcctttttgg tatccattac ccttagttga aaagaaatca
      961 tgtgttttgg tttcagtgct aagaccattt aatacaattg gatttatagc atctacagta
     1021 tatagcttct caaaccctag attttctaaa gctctattcg cattgtattt taaaaagttt
     1081 acaacctctt tttcaagccc actttctctg tatataacat ttgtatactc tatctcattc
     1141 tccataaggc tataaagtat agaatacatc ttctcttcta acatctcctt atccatttta
     1201 tcaaatgaat cgtatatttc ttgtgctagc aatcctatat acttaccatg taaagactca
     1261 tctctaagaa taagcgatat tatttctcca ctagctacca tctttccttg accagataga
     1321 tacaatggat aaaagaatcc tgaataaaac aagaaactct ctaaaaatac acttgtaacc
     1381 attgataggt ataatccctc ttgatttgta gtattttcgt attgagctaa aactaaatca
     1441 gcctttcttt gtaatgttgg ttctgtttct atccattcaa ataattcatc tatttctaaa
     1501 ttagaaagta gagtcataaa tatagaagaa taactttttg catgtatttc ttccatagtc
     1561 cccataaaag acaagacagc ttttctttgt aggttttctg ttaaactcat catagatggt
     1621 attccattat tcccctgttt tgtatccagt aatgttaaac cacccaatac ttttttatat
     1681 aattcctttt ctttattact tagttcattc cataccttta tgtctttaga aacagatatt
     1741 tcttcaggaa gccaaaattg ttttacattt tgttcccaaa aagcttgtgt aaatccatct
     1801 tcttctctat tccagttaac tgcattatgt attttatgta aattaaatgt catatatttc
     1861 tctccctata ctgaacacac tagacactca tgagtatctc ttgtcatctt tgttcttgtg
     1921 taatataaac ttttcaaacc ttttttatat gcatatatgt aatgtctagc tatatctcta
     1981 gttgtctttt catctgtaac aaacagtgtt gttgatatac cttggtcaac atggttttga
     2041 actgttgcca ctaaatctat gacctttctc atatccatcc tgtatgctga ttgataatac
     2101 aacatattat catttgttaa aaatggcatt ggatatattg tagtagagtc tccatatgtt
     2161 cttacttcta ctggctcagt aattggcatt atacttgatg tagcattttg aacataacta
     2221 atgctttgag ttggtgctac tgccattaaa tatgcattat atatcccttt ttcttttacc
     2281 tcatccaata attttgtcca gtcttctttt gttggtatat aaatcccttc aaataatgct
     2341 cttacctttt ctgacttagg tagataagat tgttcatagt attttgataa aaccttgcta
     2401 tttcttccct taacatattc tgatttatca aatccttcaa aagtttgatt tctttctata
     2461 gctattttca tagaagcttt aattgaatag tatctaacca ttgcaaaaaa tacattagaa
     2521 aaatctatag catcatctga agtatataaa atattttctc ttactaagta tccatgtaaa
     2581 ttcatagctc ctaatcctat agaatgagac ttgctgttac tattgcttac cgttggtact
     2641 ggttttatat ctgtgctatc tgctacaaat gacagtgctc ttattgctgt ttcaactgta
     2701 ctttctatag ttttattatc cattacattg gctatgttta aagaacctag gttacaactt
     2761 atatcttgtc cccattcatt ttttcctcca tacccctcta tttctgaagg tgtttgatat
     2821 tgaaatatct cacaacataa gttagacatt ttaataattc caacatcttt taatgtatgt
     2881 tctctattag ctgtatctat gtacactaca tatggatatc cactttcttg ttgcatctgt
     2941 gctatttttg tcaacatctg cctaggattt atttcttttt ttcttatatc actatcatta
     3001 accaatttat cataccactc atccatttct atctcatcaa gagaaactcc atatttctta
     3061 tacacagtat gaggataaaa tgcatatgct acttcatttt tttctgccaa ctccataaat
     3121 ttatctggta ttatagctcc taatgataat gtagcaagcc ttattttttc atctgcattt
     3181 atctttttag tatccatcaa caattcaaaa tcaggatgga atacagtaag gtacaccgca
     3241 ccagaacctt gtctagctcc catttgatta aagtaactaa atgattgttc taacatttta
     3301 gctactccaa caacacctcc agctgcacct tctatatctt tgatagattc gccagatgct
     3361 ctaagtttag ataaatttag tgcaactcct ccaccaatct ttgataagtg atttgatgaa
     3421 gatatagcat acgatatacc ttctgtacta tcttctacag atagtaaaaa acatgatacc
     3481 atttctccag ccctttttct tccagcattt aaaaaagtag gtgtagctgg ttgaaattct
     3541 tgttttacta ttttatctgc caagtttaaa gctaaatctt tattgccatt tcctagtgtc
     3601 aaagctacaa ttaacacttt atcattatat gtttctaata tttcctttcc atcattgctc
     3661 tttaatgcat aattttgata aaacttattt gcactcatat atgattgaaa ttcaaactta
     3721 tgtttatcaa tcatatcata tatatttttg ataacatcta tgctatactg atttattact
     3781 tcttttgaat aataaccttc atctattaaa tacttcaatc tttcttctag gttattaaat
     3841 tttttcaact ttggctgtat aaattcttct atgtaactac ttaaggcttc cttatccttt
     3901 tccaattggt acttgccttg ttcattttta attatcactt gattgttaag ctctatccaa
     3961 ttcattattt accgtccttt taacataatc ttgtgccata atttcactta atttgcttat
     4021 tatttctttt ggtacttatc agttgaaatc ccaaaattaa tctataatta tagaaatgca
     4081 ataagtattt ttcaaaaaca aaagcactac atatagtcag tgctttatct attatatact
     4141 acatatagta tatacacaaa tttttttata tctaaacttt tattacttta taattgacat
     4201 tatttttatt taaagaaatt tttatcaact caaatataaa acaatacaag cttattatgt
     4261 cctgtttaaa aatttaaatt atctctaaat agcttaaaaa tatattctga tatattgaaa
     4321 aaagtacttt caagatgata ttttatatat tatttagaag ttttttattt attgttatat
     4381 aattaaatat atgactattt tgctactaca tatataaaaa taaatttatg aaggtgattt
     4441 taatgaaaac tatttctaaa caagaattag aaaaaacact tgagaaacat tatttatggc
     4501 ttaaaaacaa taatgctgga gaaaaggcag atttaaaaga attaagatta gaaaatatgg
     4561 acttaagagg atactgctta agtaatattg acttttcgtg gtctgacctt atcaatttaa
     4621 atttagaaaa agcagattta gaaaactcta tttttaataa ttcttatctt tcggattgtt
     4681 cattaaaaaa ttctatatta aaaaatgcag acttaagtgg tgcaagtttt agattttgtg
     4741 acttatctaa ctcagatatt agaggagcta atttagaaaa ttcaaattta gaacatgcta
     4801 ctttaaatgg agtaatatca gatgaaagta cccgtttttt taaattgtat tgtcctgaaa
     4861 caggagcatt tataggctat aaaaaatgtt ttaattttag aatggtacaa ttattaatac
     4921 ccagcgacgc aaaaagagtg tctgctacat caaatgcttg tagatgtgat aaggcaaaag
     4981 tactgtcaat tactagtata gatggtaaag aatcttttaa aagtgctcgt gcatatgctg
     5041 atgaaaattt tatatataga gtaggagaaa cgattgtagc tgaagatttt aatgaaaata
     5101 gatggaaaga atcgactaca gggattcact tttttttaac tagagaggaa gctattggtt
     5161 atctataaaa tgctaaagtt ttataaatat ataaatacat aaacaaaata acaaaagagt
     5221 atagattata atttcaaaaa aattataatc tatactcttt tttctatagc acataagtac
     5281 attttatttc ataaattata ataaattatt tatatctgca ctattgatta tttgagaaac
     5341 tttatctata tgttctatct tattatccaa actgtcataa tacttcttac tctcatctct
     5401 atttctaaaa gaataaactt ttttatccaa ttttaaagcc tcttttataa tatctatatt
     5461 cctcttattt gtctctccta cactaaatcc tgtatctatt ataatcttgg aatcatttaa
     5521 atttctaata gccaaatcga aactctcatc acttataggc tcaaatggat tttctgtaaa
     5581 catctttatc cccatagttc taccaatttc ataatcaata tcattttcat gtattattcc
     5641 agaatacaga cctatacctt ttttagtaaa agctctataa attggtgttg ctttaccccc
     5701 accaccaatt ataaaaactt catttttaga tttattacta atttctactg aacctaaaag
     5761 actattaaag tttttatcat ccaattcata aagcgaattt ataatatcct catctactac
     5821 atcttcaggc tgaccataag ctatgacttt attatttttt ataagagcaa ctttatcaca
     5881 actttttaat gctatgtcta tttcatgcaa tgaaagtatt actgatattg acttttcttt
     5941 acttaatttt ttcagtatgt ttatgagttc caatctatgt tttatatcca agtgagttgt
     6001 aggctcatct aatataatta tttctggttc ttgaacaaga gcccttgcaa ccaacacctt
     6061 ttgtttttct ccatcactta gttcatcaaa atatcttttc tttaaatgta aggcatctac
     6121 agactccaat gcttcatcta ccattattaa atctttctta gatagactcc caaaaaatcc
     6181 tgtatatgga taccttccta tattgactat atcttgtaca gtcattaaat cacctaataa
     6241 tctatttgta agtactaaag ccattttttt tgataatgct tttcttgata tacaatttat
     6301 gtttacacca ttcaaatata tttcaccttt tattggtgat ataagtttag ataaggacct
     6361 taatagggta gtttttccag ctccattact tcccaaaaga catagtattt ctccattttt
     6421 tacttctaca tttatattac ttataacaac tttgttattg tatcctacac ttaaattatt
     6481 agttttcaac atgtctaagc cctatccttc cttttcacca ataacattac tacaattgga
     6541 gctcctaata aagatgttat cgtacttatt ggtaattcta ctggtgataa aattactctt
     6601 gatattaaat cacaaagagc tgtaactata ctgcctaaaa atattactac tggtatcaaa
     6661 actctattat ctgatgtttt aaatatcaat tttgaaattt gtggcactgc aagtccaata
     6721 aaccctatag gtcctgcaaa tgctgatact attgctgcta atatactaga tataaatact
     6781 atcaaaactc taaaaaatct tatatttatg ccaatacttt ttgcatactc ttctcctaat
     6841 aaaaaagcat ttaaaggttt tattatgaat acagtcatca aaagacctaa tataccaact
     6901 attataagta tttctacctt ttcccatgta aatccagaaa aacttcccat agaccacata
     6961 gtaaattctt ttaatttatc actatttgca aatgattgta acacatttgt tataccacta
     7021 catacatatc cggtcataat tcctattatt aaaagagttg ttatattctt taccttattt
     7081 gcaaataaaa tgactataga cattacaatt aaagctccta taaaagctgc tacaaatata
     7141 ctcattgaat ttacactctc aaatcccaat gtgtatcctc ccaaaatcat gagagctact
     7201 gataaagtag aacctgaaga tactcctaat atatacggtt caactattgg gtttttaaaa
     7261 aatatctgta ataaaatacc tgatactgct aggcatgctc cacctaccat ggttgcaagt
     7321 actctaggga ttcttatatt gtatattatt ttataatgtt ctgtattatc catacctctt
     7381 aatacaacat ctattacttc tttaaattca atttttacag agccaaatgc tacacacact
     7441 aaaaaagtaa gtatcatact tataattaat actataaata ttgttgtatt ccttattctc
     7501 attttattga taaaatccat ttgtatactc ctcttagcaa agctatatta actaaaatct
     7561 ataaaaccta tctttttgtg tcctctctgt actatttatc ataatttata taatgtttga
     7621 ttttttcacc tgtagtacct gggtggaaca tctcaactaa atctactatt aattcgtccg
     7681 tcttatctac tgattgataa taatctggag caaacaccca taggtttccg ttttttacaa
     7741 catccaaatc agccaataca ggtgcactag attttataga atctaaattt ggtgcataat
     7801 ctgaacttga tgaatataca aatatatctg catcttttgc aacttcatgc aattgttcaa
     7861 tacttactcg cccttcattt gaatctattg aagcacatgc atttattcca ccagccattt
     7921 ttatcatatt atccacatat gaatctttag gagctatata agcacttcct tcatatacag
     7981 atgcccatac aactttaggt ttagctttgt tttttatttt tttacttact tcttctacct
     8041 tttgtacagt tttatttagt tcactagtag ccatatcttc tttatcataa aatgcagcta
     8101 ttaaacttgt ccactccatt cttccaaatg gattttgctc tttatactca ttgtctacaa
     8161 cataattaag ccctaattct tttaatttgt caataaattt tgtatcagcc tctgataaac
     8221 cactactcac tatagttaag tcaggattta agtctactat tttttcataa tctggtgcta
     8281 aatttgaacc aacgtaatgt atttttccat ctttcattaa atctttaatt tctttaatag
     8341 tccagtactg ctcttctgtt gttacggctt ttatactatc cagttcatct attgttctca
     8401 gagaacaagc ctgtgttgta gatgctatta atacattgtc cacaggcgtt ctaactatat
     8461 tagcattttt atatttttca ggaattttaa tcttctttgg gattaaaatc agttctcgat
     8521 tatctccatc agttactttt ttaattccat cttctaaatc ttcaacacta aatccactag
     8581 catattttaa ttctaatttc ttaccagtac tagcttgttt attctctttg tcacttttac
     8641 tacaccctat aagacctcct accataaaaa ttaccatagc aattaaaaaa aactgcttta
     8701 aaacatttga tttaaatttc atctctttca ctccaatatc ttatttttta tgagtggcta
     8761 aaaaacttta ataagatata ccttttaaag tatatcttat taaagttata ttaaaccatc
     8821 tttaacaagt ataacttttt aataaaatta tagtgaattt tgatttcaca tgcaattaac
     8881 tacttataca cttaactacc tacgacaacg tttgaagtgg catcttcttt gtcaaaatag
     8941 ctattgttat tagtatctcc aagctaatag gatagttccc aacacttacg attagcatct
     9001 gagtcactac gttggcaaac taatagaaat ataacattta tttgatgatg ctttagatac
     9061 agtacaactc tatcaaattg cctatgttgt tttatcctaa tcattgattt taatttaagc
     9121 agttttatat acccatttct atatgcacaa tatcttttga gtaacgtgtt gacaaactat
     9181 tttggttagt agtatagcta tgatagttaa ttttctttga tagataagtc cgttacatcc
     9241 aaaattctta ggaaaatatt ccttttattc actgtttgta taaacctgta cttataagct
     9301 cttaacatat tatcactatt cttcttgatt ttctatgtat tgttccaaat cttccaatgt
     9361 tgcaccacca gttgttacta aacaataact tttactccaa aagtattctt tccataagta
     9421 tttgcggata cttggaaact cattctttat ccttctgctt gatgcagatt tataggcatt
     9481 taataacttg gaaatttctg tattaggctt tgctttgaat aataagtgaa catggtcaac
     9541 ataatgattc cattctatca acaccacccc ataatttggt gcaatatcta caaatatatc
     9601 ttttagtcta tcagatatag aatcatctat tacttttctt ctatatttta tacacataac
     9661 taaatgataa tgcaataaga agactgaatg attattacta tctaatttca ttataattac
     9721 ttcccttcag cttttactac taaattaatg atataatacg tttttatgaa aatcaagtaa
     9781 aaaaaagaac ctatcaatta tgcttacatt cttaactttc ttaaataaat acaaaaaact
     9841 gacaactaaa aattgtcagt ttttataact atacactaaa ataaattgta atctataggg
     9901 aatgatacct ttatgactgt accaacattt acttttgatt ctaatattaa acctaaacct
     9961 aacttattac ataacttttt acatagatat aatcctattc cagtactttt tccaaatact
    10021 cttccattct ctcctgtaaa ccctttttca aataccttat taatatctct ctcttctata
    10081 cctactccat tatcttctat agttaacact attgagtttt ctaccctttt tgcacttatt
    10141 tttattaaac catcttttga ctttatatat tttatagaat ttaccacaat ttgatttaat
    10201 ataaactcaa cccatttcga gtcactatat acagtcttat taatttcacc aagttctaat
    10261 tttatatttt tgtttataaa gtctctatag tttcttttga taacttcatt tacaacttct
    10321 tcaaggttta gcttttttat tatataatct ttatttacat tatcaatttt tgaataatat
    10381 aatacttgtt ccacaaaatt ttcaacttta tctagttgag tatctatctt tttagtaatc
    10441 tcattactat tattttctat aataagttta ctagatgcta taggcgtttt tatttcatga
    10501 acccaagttt caatatattc tttatattct tcttgattat cattataata tttaatattt
    10561 tcgtgcatgt ttctacttat tacttttaaa atatcattta ttttttctcc agttacaaaa
    10621 tttacatcat caataacttc tggcaacaaa tattttttat ctaaagcatc taaaatatta
    10681 tctatagaat taaagtactt cttatattgt aaataatcaa ttaacatata agtaaataat
    10741 ggtaaaaacc atatacaaaa taacataaat attaaaacga catttatttt actataaagc
    10801 atgatacctg atgcaattaa aaatataatt aaattagtac ctagaaaaac aactttatca
    10861 cctatatact ctcttaatct catgatatta tataccctaa accacgtctt gtctctatca
    10921 aattttctat gcctatttca tctagctttt tacgtagcct tgttatattg acagtaagag
    10981 ttctatcgtc tacaaaccaa tttgtgtgcc atagatactc cataaatgaa tctctagata
    11041 ctattgaacc ttgattctcc actaaataag atagtatttt aaattcattt ttagttaact
    11101 ctatttcttt atctttatat ctgagtgttg cattaaataa atttaattca atacccttaa
    11161 atattaaagt attatttaca ttcgaattta atccacttct ttttaacaat gcaacaatcc
    11221 ttgctaaaag tatttgtgtt ttatatggct ttgtcacaaa atcatctgct cctaaattca
    11281 tgctcattag ttcatccata tcagtgtttc tacttgttac aattattatt ggtatattcg
    11341 attctttacg taatactcta catatataat atccatcata tattggcaag tttatatcaa
    11401 gtaatactaa attaggtttt tcacttaata taaactctac tatattatca aattcagtag
    11461 gagccataac ttcatatcca tacctctcta aaaagctttt taattcatct ctgattactt
    11521 cttcatcttc cactataatt attttttcca ttaatttttc accctttatt ttcaaaaaat
    11581 ctatttacag tatgattttt actgtaaaca gattttagtt agttgtatta attaagcttt
    11641 tttataacta tatttttata cccttgataa gttgtataaa aatacactgc atatactaac
    11701 attaaaattc ctactgtcat aagtgatgaa gctcctatat ctggcttatt aagccttgat
    11761 ataaaattat tagccacttg tattcctact actgagtgaa ctaaagctag tccaacaggt
    11821 atgctaaagt acattagagt ttgtgtaaat atagttttac ttatactatt tttacttgct
    11881 cctagttttt ttaaggatac atatctatct atactatcac tagcctcact taattgctga
    11941 agtgcaagga ctgccatact acttattaaa aatactattc ctaaatagat tccaacaaat
    12001 agtattgtag aagtcatacc attattatca ttatacatag catttcttga tccacctgat
    12061 acagttccaa cttcataaaa acttgtagaa ctatactttg attttagcct tttatcaaaa
    12121 ttttcttgtg acttttcatt attatctgaa gcgaagttta tatttactat acttgaagat
    12181 atctccatgc tatcacacaa tttgtcattt acaactacag taagagcatt tgtttctgta
    12241 aaatcagttc ttaaattatc ttttattact tttttatttt ttatattata tgctttatta
    12301 tctattttta taattttatt atttttcatg tattcttcta tcgaaggtac tacctctgta
    12361 gcattagatg ttattaatac ttcatctttt gataaagtta ttgacttttc acctcgtaac
    12421 tttcttattt cattatactg tgaagatttt atatatgcta cttcaaagtc cactctacca
    12481 gaatattgtc cttgcataac atctgaaagt ttattaccat ttatatattc atcatagtaa
    12541 gcaactttat cacctttatt aaacttaatt ccaacctttt caaaagattc ttttatattc
    12601 ttcactttac atttttcatg tacaatcatg gttgcacttg catcaaatgg agttgcacct
    12661 tttagtcctg catttaaagc tgatttaaaa ccaaaccctg ttgatagtat tcctattgtt
    12721 aaaaataaca ttaaacaaat aatagatatt gatataaagt ttgtattaat cttactattt
    12781 atttgcttta ctacaaatat gtttaatccc ttaaagtaaa tgttcttatt cttcttaaca
    12841 atatataatg caaagcctgc taaactatag aagaataata tagttccaat tacacctaat
    12901 actattgaca taataaactt aggattttta ggatttaatc caacttttaa aaccatgcta
    12961 tatgcaataa ttaaggaaat aatgcataat ataaatgtaa ttgtatatac aaatggactt
    13021 ttaaatttta tatcctctgt ttttcttcct attgttaata aatctattat tttatattta
    13081 gaaattatat aagtattgaa tatcatcaca agtaaaaaca ttattccaaa atagacaata
    13141 gttttaataa ttgatgatat cgatattaca aaattgtatt cactcatatt aaattcaaat
    13201 aactttaata ataatattga taacccttga gatactaata acccaagtat tagtccactt
    13261 actaaagaaa ctattccaac tattatagtt tcaaaaacta atattttcga tattttaact
    13321 ttttccatcc ctaaagtcat ataaattcca agttccttat tccttttttt gactaagaag
    13381 ttattagcat aaagaattaa actgcataaa ataactgata caaatacaga catgtatgaa
    13441 atcatattct taagcatttc tacatgagat tttgttgaag cattcatttc cattaaagct
    13501 ttttgagatt ctatagagtt aaaactatag aaaatacaaa ttgctaatgt taaagtcaaa
    13561 aaatatatgc tataatcctt aaagcttttt ctaacatttt taattgctat tttagaatac
    13621 atcgttatca tctcctccta ttagagttgt tacttcaatt attctattaa aaaactcttt
    13681 tcttgaatca gctcctctta ctaattctgt aaatattttt ccgtctttta taaataggat
    13741 tctatgggca taactagctg taaaagcatc atgagttacc attaaaattg ttgcttttaa
    13801 gtcttgattt agactttcaa acctctcaag taataatctt gctgattttg agtcaagtgc
    13861 tcctgtaggt tcatctgcca aaatcaatga tggattagta actattgctc tagctgatgc
    13921 tactctttgc ttttgtcctc ctgacatttg ataaggatat ttatctatca ctttttctat
    13981 ttctaagtat gtagctacat ctcttatttt tgaatcaatt ttagatggtt tttctccttt
    14041 aattgttaat gccaatgcta tattttcata agctgttaaa gtatctaaaa gattaaaatc
    14101 ttggaatata aatcctaatt cattttgtct gaacttatct aagtttttag attttaatct
    14161 tgtaatatca ttattattta ttattatttt acctattgtt acattgtcta ttgttgatat
    14221 acagttaaga agtgttgtct tcccactacc acttggaccc attatcccaa caaattctcc
    14281 ttcatcaact ttaaagctta tattatctat tgcttttgta atattatctt tgtttccata
    14341 atatttttct attttttcaa cacttaatat attcttcatg ttctatttct cctttaaatt
    14401 aattttctta tatctctatt gtaatttttt atttaatttt tttaaa
//
LOCUS       DAGSRM010000099        14272 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.099, whole genome shotgun sequence.
ACCESSION   DAGSRM010000099 DAGSRM010000000
VERSION     DAGSRM010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14272
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.099"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(228..1172)
                     /locus_tag="MJE33_003053"
     CDS             complement(228..1172)
                     /locus_tag="MJE33_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBY9648972.1"
                     /translation="MQKNKKEGALLKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTP
                     LQIITLRFFLAAIIMNLLFFKQIRANMGKKLLKAGGILGIFLFLAFTVQTIGLMYTTP
                     SKNAFITAANVVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFL
                     TLICSFGFAFHIFFTSEFAKDNNPMALTAIQFTVAFLMSVVVQTFAGQLKMEAELSGY
                     MGTMYLAVFSTTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLV
                     IGSILIFVAIITAETKLSFLKSKKVKLKDSEESSLESI"
     gene            1558..2439
                     /locus_tag="MJE33_003054"
     CDS             1558..2439
                     /locus_tag="MJE33_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBY9648973.1"
                     /translation="MVKKRKKKTVALTILVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAVKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPNRSLNLDTFVNELNKTDEAMKKVLGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
     gene            complement(2500..3708)
                     /locus_tag="MJE33_003055"
     CDS             complement(2500..3708)
                     /locus_tag="MJE33_003055"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBY9648974.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     KIESQLKEGSVNPRDIKMDLAREIVTLYHGEESAKEAEERFKSVFQKGQIPEDIQTIQ
                     VKEDGFDLIEVLVSNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     misc_binding    complement(3781..4033)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(4185..5174)
                     /locus_tag="MJE33_003056"
     CDS             complement(4185..5174)
                     /locus_tag="MJE33_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HBY9648975.1"
                     /translation="MIYGGSVLNTTKKLTEAALLSSLFIVVTIIAVSTGFGYAIYLDF
                     IVPVFFCIICLKCDLKYTILSGITSLLIISLVLGNLGTAIWASQSVLLGIMCGYLINK
                     PTMVMDDLVYGSVFGVIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLM
                     IALFPFGMVFCIYLLSLILGNKLHILDSNSLKKFLIFKNFRNLNQFLCCSKKAFYICV
                     SYLAIFEVLKLLNVKVDSVYLKTIFISITYISVYFIIRDSCMSLQNFIIAKFRKLLYA
                     RILFLIIILLLFFIFDVTVIGLIIINAFLNKKINIRLSQSNIVNKQINLLIEK"
     gene            5326..5766
                     /locus_tag="MJE33_003057"
     CDS             5326..5766
                     /locus_tag="MJE33_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY9648976.1"
                     /translation="MFKIKHFNDLSLDEFYEIAKSRYEVFACEQKIFSLNDYDDIDKS
                     SYHIFLKENGLICAYARIIPKEYSSYNDVSIGRVLVLSSHRRKGLAKQMMDCAIDFIK
                     VNLHENNITLSAQTYIKNLYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            6093..6320
                     /locus_tag="MJE33_003058"
     CDS             6093..6320
                     /locus_tag="MJE33_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBY9648977.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            6346..8466
                     /gene="feoB"
                     /locus_tag="MJE33_003059"
     CDS             6346..8466
                     /gene="feoB"
                     /locus_tag="MJE33_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBY9648978.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKEDQFVIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKYKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEARKKGITIDKKLLEDSLG
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIENVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKDSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
     gene            complement(8599..9417)
                     /locus_tag="MJE33_003060"
     CDS             complement(8599..9417)
                     /locus_tag="MJE33_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBY9648979.1"
                     /translation="MEKQYFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIGMSLEEIKEYIQNRTPQLCVKMLKEEEKKIREKIKKLRKIS
                     SKMQNRINITVEGISKMNSEEIYISKKFEEYLVLSSDLSNMSNSDFMMELIDFTNYCK
                     SKNLYQGYELGVIVSNENIKNGDYLSISSFYLKIDKKIKDKKLYVKPEGMYACIKHIG
                     KYEDSYKSYEKLKKYIYENEYKIVGNSYEESILDFFCESNEDNYMTEISIQVSR"
     repeat_region   10076..11165
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=10143..10171
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(11824..13917)
                     /locus_tag="MJE33_003061"
     CDS             complement(11824..13917)
                     /locus_tag="MJE33_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBY9648980.1"
                     /translation="MNKHNFEVILNQLQINIYVTNIHTNEIIFMNKKMKEEYNILDPE
                     GKVCWQVLYPEKNSTCSFCKVLELLKNDKKGVLIKWYEKCNKLNRVFENYDSLITWQD
                     GTVVHMHQSIDIANSTSLNKPIKINEFHEISNNKEEKGVFNFSRDNFDYNSTLLYDAL
                     IRGTDEYIYICNMKTGVFRYSPSQVELFDLPGEIVENPLVYWKKIVHPEDWNRFYKSN
                     TEIGKNQMDYHTVEFRAKNRSGEYIWLRCRGQLMRDEFGEPSIFAGIMTQLGKQNKID
                     SLTQLLNYHEFMSVFEDKISNPMIEKLCIVLLNIDDFKNVNEMYDRDFGDNIIKTLAQ
                     SIQSILPDNAELYKLDGDEMGILVDNVEENEILTLYNQIQNMIIHLQLWRKYGLNITI
                     SAGCVIYPKHGDTVKELYKCASYSLQYAKEHGKNRLVFFSQEILKNKMYSLEMMRDLK
                     ASINDDFRGFSLRFQPQVDTESHKIIGVEVLLRWTNDKCKAISPLEFIPILEENDMIN
                     IVGAWVLRMALRTFRKWIDYYPFFKVSVNVSAVQILEDTFIEDIVKIIDDENFPYQNL
                     VLELTESHTVQNMSILQFKFKVLQDLGIYIAMDDFGTGYSSLEVLKFSPIDIVKIDRV
                     FVKDILKSKFDATFIHFIVAICHDVGIKVCLEGVETQEEYDLVKQIKPDYIQGYLFGK
                     PQTATEIFDLLKLDN"
BASE COUNT         5137 a         2079 c         1683 g         5373 t
ORIGIN      
        1 ttcataatta atttcctccc aattttatat aattaaattt ttatttttaa gtttaataac
       61 ttgctacaag aatatatata ccccagactt tatagcttaa acactaaaaa taaaatttat
      121 ttcctaatta attttatcac tttgaaacac cagtatcaac caagttttga tgtcaaattt
      181 ctacataata cataatttat ttgattaatt aggaaatagt tgtcatatta aatactttct
      241 aatgaagact cctctgaatc tttaagtttc acttttttgc tctttaaaaa agataattta
      301 gtttctgcag ttatgattgc aacaaatatc aagatactac caataaccaa ttttgctgta
      361 ataagttcct ttagaattat tatagaaaat attgtaccaa atacagcctc tgtggataat
      421 attatagctg ttctagttcc atcaactctt ttttgacata tagtctgaaa taaaaatcca
      481 attgtagtac taaatacagc tagatacata gttcccatgt aaccagataa ttcagcttcc
      541 atctttaatt gacctgcaaa tgtttgaact acaacagaca ttaaaaacgc tactgtaaat
      601 tgaatagctg tcaatgccat tgggttgtta tcttttgcaa actcacttgt aaagaatata
      661 tgaaatgcaa atccgaatga acatataagt gttagaaaat ctccaaaatt aatagagaaa
      721 tcagcttcta aagaaagtat cccaattcct ataagagcta ccaaactact tattattcct
      781 attttatcaa gttttcttct ataaagtata aatcctatga atggaactat tacaacatta
      841 gctgctgtta taaacgcatt tttagatggt gtagtgtaca taagtccaat agtttgtact
      901 gtgaatgcta gaaataaaaa tattcctaaa atccctcctg cttttaataa ttttttaccc
      961 atattggcac gaatctgttt aaaaaacagt aaattcataa ttatagctgc caagaaaaat
     1021 cttaaagtta tgatttgtag aggtgtaagc ccaccgtcta atgcaaattg ggttgctaca
     1081 aagccacttc cccatataat tgctataaat attagtccta tttctccaaa gtattttttc
     1141 aataaagccc cctccttttt atttttttgc aaaattataa accccctaaa cagcaagaga
     1201 gtttttctat aagcattact ataaattaat atagtaaatt ttttcagtat tttaaatcta
     1261 aatttacata cattttgagc tcaaatttat atccactatt taacagtaat atattaatca
     1321 ttttttgtaa acaccttttt aataaatttt tatatttttt atattttttt acctatacta
     1381 aattttttta tagatatttt taatatatat gatactttaa tattatgcaa ataatgtcat
     1441 ataagttaca ctttagttat cttagttaca actttgtcgg tttacttttt ctactttctt
     1501 cgatataatt ataatatatt tttgatacaa ttcgacaaaa gggggaatgc tgtaattatg
     1561 gtaaaaaaaa gaaaaaagaa aactgttgca cttactatac ttgttgtaat agttattggt
     1621 attttttcta tggcatgggc ggacatgtct agaaaaaaag aaatgaaaga aactcaagaa
     1681 agacttaaag ctgaaagatt agaaaaagaa agaattgaaa aagaaaaaaa tgaacctatt
     1741 attggtgcaa aaaaagaagc tgtaaaagaa tatggatatg acgcaaaaat agtttgggac
     1801 aaacttaaca aatacgacta ttcaaacaat ggagaaaaga tagtctttct tacatttgat
     1861 gatggaccat ctactactaa tactccacaa gttctagata tcttgaaaag acatggtgtt
     1921 agaggaactt tctttattaa gggtgattct cttgaaagaa aaggagctaa tgaaatttta
     1981 aaaagaacat ttgacgaagg aaatgcaata gcccatcact cttatactca tgactataaa
     2041 aaactatatc ctaacagaag cctaaatctc gatacattcg taaatgaact taacaaaact
     2101 gatgaagcta tgaaaaaagt cttaggaaag aatttttcct caaatgtagt tagatgccca
     2161 ggcgggtata tgtcatggaa aaatatggaa cctctaggaa actacttaaa agagaaaaac
     2221 atggcatcaa ttgattggaa cgctttaaat gctgatgcag aaggtaaaaa gaaaaatgca
     2281 caagaattat ttgaacatgc aaagaaatct tctgaaggca aagaaatggt tgttctttta
     2341 atgcatgata cttatggaaa acaagaaaca gtaaatgcat tggaccaaat tataacatat
     2401 tttaaagaca atggttatca atttaaaatt ttaatataat aaaaaagcaa gtttattgaa
     2461 tgtacatttt atactcaata gacttgcttt tactttactc tattttacta gctcgatttt
     2521 tacaaatttt ttctttccta tttgaacaac taattcactc tcttttacaa tagtgcttaa
     2581 atcctctact ttttcaccat ttacctttac tccaccttga gaagctaatc ttctaacttc
     2641 actcttactc ttaacaatct cattagaaac tagtacttcg attaagtcaa atccatcttc
     2701 cttaacttga attgtttgta tatcttcagg tatttgacct ttttggaata cagatttaaa
     2761 tctttcttca gcttctttag cagattcttc tccatggtat aatgttacta tttctcttgc
     2821 taagtccatc tttatatctc ttggatttac tgaaccctct ttcaactgag attctatttt
     2881 attaacttca tcaggatgaa catctgtcac taaattgtag tatttaatta taagttcatc
     2941 aggaatttcc atagattttt ggtacattat tcccgcttct tcatctattc caatatagtt
     3001 acctaaactc ttactcattt tttctttacc atctaatcct tctattaaag gcatcattat
     3061 tactatttga gactccatac caaattctct ttgtaatgat cttcccatta gtaaattgaa
     3121 tctttggtca gttccaccaa gttctatatc agcttctaat gctatagagt catatgcttg
     3181 cattaatggg tagaagaact catgtactga tattggcatt tgtccttcat atctcttttt
     3241 aaaatcttct ctttctaaca ttctcgcaac agtaatagtt gctgctaatt ttattacatc
     3301 ttcaaaattt aacttagcta accactcgct attaaatctt acaatagttt tttctttatc
     3361 taaaactttg aaaatttgtt cttcataagt ttttgcattt gcaagaactt gctctgttgt
     3421 taaagctttt ctagctttag attttccagt tgggtctcca atttttcctg taaaatctcc
     3481 aattattatt actatttgat gacctaaatc ttgtaattgt ttcatctttc taagaactac
     3541 tgtatgccct aaatgtatat ctggagcact tgggtctaga cccaatttta ctatcatagg
     3601 ttttccttct ttttctgatt taataagctt ttcccttaac tctttctcat ctattaaatc
     3661 atctacacct ttcataatta ttcgcatttg ctcgtctatc gatttcattt ttcattcctc
     3721 ctaaaatatt tatataatct ataattttta tttttataat aaaaaaagct ttcatcctct
     3781 taaaaggacg aaagcattgc ttacgtgtta ccaccttagt tcatcaatat ctcacgatat
     3841 taatctctta gagtacaatt acatatactc gtgtactata acgtgtacta atccgatatc
     3901 tcttaatggt tgttatcttt cagagatagt gctctgagat gtattcaaac taaatcaatt
     3961 tattccattt caccaaactg gaactctctg ttaaataaaa tctagcccta cttcttctct
     4021 tcatagcatt tatatattca gattatttca tcaattacaa gaaatttttc ttaacttact
     4081 cataatatat tattattttt atattttgtc aacctttttt attattatat acatgttttt
     4141 ttacattata ctactttttt atatttttta ccaataccca aaatttattt ttcaatcaaa
     4201 agatttattt gtttattgac tatattactc tgactcaatc ttatatttat tttcttattt
     4261 aaaaatgcgt ttataattat taaaccaata actgttacat caaatataaa aaacagcaat
     4321 aaaattatta tcaaaaataa aattctagca tataataatt ttctaaactt agctattata
     4381 aagttttgta gtgacataca tgagtctcta atgataaaat agacacttat ataggttatt
     4441 gatataaaga ttgtttttaa ataaacagaa tcaactttaa cattcaacag ttttagaact
     4501 tcaaaaatgg ctagataaga aacacatatg taaaaagctt tttttgagca acataaaaat
     4561 tggtttaaat ttctaaaatt cttaaatatt aaaaattttt ttaaagaatt agaatctaaa
     4621 atatgcaatt tattccctaa aattaaactt aataaatata tgcaaaacac cataccaaaa
     4681 ggaaacaatg ctatcattaa ataataaata aaatttgtat acccattaaa ataaatccat
     4741 ttgctatatc cttgaaattc tttcataaaa ctataaccta ttaatgttga agcatatata
     4801 tcaatgaaaa ccattacaat aaccccaaat actgaaccat atactaaatc atccataacc
     4861 attgtaggct tatttattag atagccacac attataccaa gtaatacact ctgacttgcc
     4921 caaatagcag ttcctaaatt tccaagtacc agagaaatta ttaaaagcga agtaattcca
     4981 gataaaatgg tatactttaa atcacatttc aagcagatta tacaaaaaaa tactggcact
     5041 ataaaatcta agtaaatagc atatccaaat ccagtactca cagctataat tgttactact
     5101 atgaataaag atgacaataa agctgcttca gttaattttt tagtagtatt caaaacactg
     5161 cctccataaa tcataaattt tctaactaaa gttttgtttt taatatagta tatcactttt
     5221 taaaaatatt tttaataaat ataaatttta ataaaggtat ttatgttaaa aatggtataa
     5281 tgtatcttaa tattcagttt tttaagatgc taggaggtaa ttactatgtt taaaattaaa
     5341 cactttaatg atttgagttt agatgaattt tatgaaatag caaaatcaag atatgaggta
     5401 tttgcttgtg aacagaagat attttcatta aacgattatg atgatataga taaaagctct
     5461 tatcacatat ttttaaaaga aaatggattg atttgtgctt acgctagaat aattcctaaa
     5521 gaatacagct cttataatga tgtttccatt ggtagagttt tagttctttc tagtcataga
     5581 agaaaaggat tagcaaaaca aatgatggat tgtgctatag attttataaa agttaactta
     5641 cacgaaaata atataacctt atcagcacaa acttatataa agaaccttta cttatcttgt
     5701 ggttttaaag aaatctcaga agtatacgac gaagcaggta ttgaacatat taaaatgaga
     5761 ttatagtgtt gacaagctaa tattgttaat atataattta agtataaatt aaatattgaa
     5821 ttgatattta ggtatttgaa aataccttac atagagtcag aaggcttttg ctttctgact
     5881 tttttattat caaaattcaa taattatatg gattcaaagt gttaattttt attctatatt
     5941 ctactttccc caaaagtaag aattataccc aactttgtat gataggcatc ttgcctatct
     6001 tttttttgta aaagtaacaa ggtaataata taatgcatag tttaaaataa gaagattagc
     6061 ccaggctaaa tttcaagcat ggaggtgcta ttatgaaaaa ccttaacgat attcaagtga
     6121 aaaaaactgt caaagttgaa gatatattat ctagtggtaa tttaagagaa agaatgttag
     6181 cgcttggtct tactagagga gctgttatag atgtagttag aaaaggtcca aaaaataatt
     6241 taactgtcta taaaatcaga ggtagcaaga tagctctacg acaagaagaa agtagcttaa
     6301 tattagtttc tgacatttaa aaatttctag caggaggata ttaatatggg gttgacacat
     6361 aactcaacta aaatgagttc tttaaaagac atgtttgata tagacaataa ggaagaccag
     6421 tttgtaattg cattggctgg caatccaaat acaggtaaaa gtactgtatt taatcatctt
     6481 acagggctta gacagcatac tggaaactgg ccaggaaaaa ctgtagctac agctcgtggt
     6541 aattttaaat ataaaaatac cgaatatgca ttaatagatt taccaggaac atattcttta
     6601 tttgccttat cacaagaaga aattgtagct cgtgatttta tatgttttgg taatccagat
     6661 gcagtaatag tagtatgtga tgctacatgt cttgaaagaa atttaaattt agtatttcaa
     6721 gtgatggaac ttacagataa agttattttg tgtattaatt taatagatga agctagaaaa
     6781 aaaggcatta ctattgataa aaaactttta gaggattctc ttggtattcc agttatttta
     6841 actgctgcaa gaaatggctc tggtatggat gaactattgg atactttaaa tgatgtatct
     6901 tttgacaaat ataaattaaa taataaacct gttagataca atgaaaatat agagaatgtg
     6961 gtaaaatcaa ttcaaccaga attggataat attataccag gtataaattc tagatggttg
     7021 ggacttagac ttattgatgg cgatgaaagt atatttgaat ctatgagtaa ttatatagac
     7081 aaagattcta tagatgctat taatgaagta aagaaaaaga ttccagacaa tataaataaa
     7141 cagaaaataa gagatgagtt tactaaaatc aactatgatt atgctaaaaa actgagtgat
     7201 gagtgttgtt ctaatgttgc taaaaaatca actgatagag aagaaaaagt agataaaata
     7261 ttaacatcta aaatatttgg gcttccaata atgctcttat tattaggtac tatattgtgg
     7321 attactatag aaggagctaa ctatccatcc actttattat ctaatttatt actcggattt
     7381 gagccaagta tttcgggtat attaaatagt attaattgtc catcatggtt aaatgatatg
     7441 ttggttttag gattatacag aacattagca tgggtaatct ctgtaatgct acctcctatg
     7501 gcaatatttt ttccattatt taccctactt gaggattttg gatatttgcc aagagttgca
     7561 tttaacttag accacctatt taaaaaagct tgtgcacatg gaaaacagtg tttgactatg
     7621 tgtatgggat ttggatgtaa tgcagctgga gttattggtt gtaggataat tgactctcca
     7681 agggaaagac ttatagctat acttacaaac aactttgttc cttgtaatgg tcgttttcct
     7741 actttgatag ctatatctac aatatttttt agctcagtta taaccaactc tttcgtatca
     7801 agtgtagcaa ctgcactttg cataacttta ctgataattc taggtgttat aataacattg
     7861 ttggtttctt atactctttc taaaacgcta ttaaaaggag ttccgtctac atttacatta
     7921 gaacttcctc cttatagagt tcctcaaata ggaagaactt tatatacctc tataatagat
     7981 agaacaatat ttgttttagg aagggctgta atggtagcca tacctgctgg tgtcataact
     8041 tggatatttg caaatatata tataggagat ttaagtatat tatcacatgt tgcaaacttt
     8101 ttagaccctt tagctaaatt aattggtctt gatggtttta tattacttgc ttttattctt
     8161 ggattcccag caaatgagat agttgtacct atacttttaa tggcttacct agcaactggt
     8221 tctatgatag aattagatag ctttagtgct ttaggtcaag ttcttagaga acatggatgg
     8281 acttatttga cagctttaaa tgttatgctg ttcagtttac tgcattggcc ttgtgcaaca
     8341 acacttttaa caataaaaaa agaaactggt agtttaaaat ggactgcctt aggattctta
     8401 atgcctacta tacttgcttt tgttgtttgt tttttaacaa ctacagttta caatttattt
     8461 atataattag tattaactga cagtagacat atattagaga aactatttaa aaatttatat
     8521 actttattat aaagcataga gatatattca atgataagta aaattactta acatttgaat
     8581 atatctctat tttaattctt atctggatac ttggattgaa atttcagtca tataattatc
     8641 ttcattactt tcacaaaaaa agtcaagtat actctcctca tacgaatttc caacaatttt
     8701 atattcattt tcatatatat attttttcaa tttttcgtaa gatttatatg aatcctcata
     8761 ttttccaata tgttttatgc aagcatacat tccttctggc tttacatata gctttttatc
     8821 ctttattttt ttatctatct tcaaataaaa acttgatata cttaaatagt ccccattttt
     8881 tatattttca ttacttacta ttactcccaa ctcatatcct tgatacaaat ttttagattt
     8941 acaataattg gtgaaatcta ttaattccat cataaaatca ctattactca tattactcaa
     9001 atcgcttgat agaactaaat attcttcaaa ctttttggat atataaattt cttcactatt
     9061 cattttagaa attccttcaa ctgttatatt tattctattt tgcatcttag aacttatctt
     9121 tcttagtttt tttatttttt ctcttatctt tttttcttct tctttaagca ttttcacaca
     9181 aagctgggga gttctatttt gtatatactc ttttatttcc tctaaactca ttcctatttc
     9241 tctcaatgat tgtaatatat caagtgtatc atattgataa actgagtaat atctataatt
     9301 atttttatct ttatattcag gagaaaaaat acctattttg tcatagaaaa tcaatgtctg
     9361 cttagatata ccacacaatt tagcaaattc acctgttgta aaatactgtt tttccaaggg
     9421 ttatacactc cttttctttt tgttgtctat atagttacta tattctttat tatttactaa
     9481 atcaagtagt tattatttta gaaaatcaat atgattttga ttttaaagct taaatatcag
     9541 ttagatgtca gttataaata ttttatccat tcccatcata aaaaagatat cccctcttaa
     9601 cttttggatg gaatattata tttatacttg ttagagtgaa aattgtaata ggtgctacaa
     9661 taagatattt aattaaacat aaagttaatt attgtaataa aaaatcttta gataaattag
     9721 agttaaagta tggaaatata gatagagaaa aagctgttaa actagaaata ttttattaat
     9781 atttaataga acttgaatat atagaaatga gtttattaat aagaagatta gatactgcaa
     9841 taacatcatt agcattagta tctattataa caatagcatc atattattta atcagaaaaa
     9901 atgtgtaatt atgtaagcta tttataataa aacactgata aaattaataa taaaaaggta
     9961 ataggatttc tatactacct tttggttttt ataatatagt gtaattttat tatatttttg
    10021 tatgctaata taactagata atcataagat aacacattaa aagaaaatta taaaaactta
    10081 cattctgtat agttaatata aaacataagt aatataaatt tatgtaaagc aacaaaagat
    10141 ggatttacat tccatatagt taatataaaa cgatttcaaa tgattagaat aaaaatatca
    10201 gaattgctat ttatattcca tatagttaat ataaaactaa gaatggaaaa ggaaatagga
    10261 gttcatattc caacatttac attccatata gttaatataa aactatgtat cccccattaa
    10321 aaaggcttag attaatttct atttacattc catatagtta atataaaact caaacatatc
    10381 taagttgtat ttctcacctt gtaggtcatt tacattccat atagttaata taaaacaagg
    10441 attttgaatg taatttatca taagggtggc agatttacat tccatatagt taatataaaa
    10501 catgaaagat aattggaaaa ctaaagtgca atctaaaaaa tttacattcc atatagttaa
    10561 tataaaacgg agaattatta aaaggagttc ccgaagcaga tagaataatt tacattccat
    10621 atagttaata taaaaccatt gttattattg tggttctccc ttagctcata atttatttac
    10681 attccatata gttaatataa aacatgatat tattataata ttataaatat aataatgagg
    10741 atttacattc catatagtta atataaaact agatatttaa aataagcgaa agggggtcat
    10801 aaaaatattt acattccata tagttaatat aaaacattct aaagatattt tgccttgcag
    10861 ttgggatttc gaatttacat tccatatagt taatataaaa ccatcatagg ggctaatata
    10921 gttaaaggtg ttggcgtatt tacattccat atagttaata taaaacggtt tggttacaag
    10981 gtatagtaga aggatatagt ggaatttaca ttccatatag ttaatataaa acatggaact
    11041 aagagagtac accatcacat gatactcaaa tttacattcc atatagttaa tataaaacta
    11101 aagctggaaa agaaacagct ataatgagta ataataattt acattccata tagttaatat
    11161 aaaaccccaa aataaactta gtattttcaa tacttacacg tatacaactc tcttaaattt
    11221 gcagtgaacg agcaatagtg caattgataa cgcttatcat acactctcaa cgccttatat
    11281 ttcaactgtt acgccaaatt ttttcacaaa tatcgctcac tgcaaaattc ctataatttt
    11341 attatatcat aaatatatta cttttgaata ttgatactaa tttataccat aataaaagca
    11401 aaaaaacgta atctatctac attagattta aattctataa cttaaagttt tttacataat
    11461 atttatgctt gtttaatcaa tctaatatat cattcttgta tattgctttt aatagtcttt
    11521 tttcttaaat aaatatctat ctgaacaagc atagtttaat taaaaacttt tgtattccct
    11581 taaaactgta tctacctctt attattttat ttatgatata ttatttttaa tctaaaaata
    11641 gatatctatt tttattatag ttatccattt ttaaaatact ttaatcatat actactccca
    11701 atttaacatc cattgaaatg ctattttctt atattacaat ttataattga tatatcttca
    11761 atataattat ttaatgtatt tttgatttta aagcttttca gtgatattat tgataaatta
    11821 gaattaatta tctagcttta aaaggtcaaa gatttctgtt gctgtctgcg gttttccaaa
    11881 taaatatcct tgtatgtaat ctggctttat ttgtttaact aaatcatact cttcttgtgt
    11941 ttctacacct tctaaacata cttttattcc tacgtcatga caaattgcaa caataaaatg
    12001 tataaatgta gcatcaaact tactctttaa tatatcttta acaaatactc tatcaatttt
    12061 cactatatct attggagaaa attttaaaac ttctagagac gagtatcccg taccaaaatc
    12121 atccatagca atataaattc ctaaatcttg taaaacctta aatttaaact gtaaaatact
    12181 catattttgt actgtatgac tttctgtcaa ttctaaaaca agattttgat aaggaaaatt
    12241 ttcatcatct ataattttta ctatatcttc aataaatgtg tcttctaata tttgaacagc
    12301 tgaaacgttt acactcactt taaaaaaagg atagtaatca atccatttcc taaaagtacg
    12361 taatgccatt cgaagcaccc aagcccctac aatatttatc atatcatttt cttctaatat
    12421 tggaataaat tccaaaggag aaattgcttt gcatttgtca ttagtccacc ttaaaagaac
    12481 ttctacacct attattttat gactttctgt atctacttgt ggctggaatc gaagactaaa
    12541 ccccctaaaa tcatcattaa ttgatgcctt taaatcccgc atcatttcta atgaatacat
    12601 tttatttttt aatatttctt gggagaaaaa aacaagcctg tttttaccat gctctttcgc
    12661 atattgtaaa gagtaactag cacacttata aagttctttt actgtatctc catgctttgg
    12721 atatattaca caaccagcag aaattgttat atttaatcca tattttctcc atagttgaag
    12781 atgtataatc atattctgta tttgattata tagagttaga atttcatttt cttcaacatt
    12841 atcaacaaga atacccattt cgtccccatc aagtttataa agttcagcat tatctggtaa
    12901 aatagattgt atagactgtg ctagtgtttt tattatatta tctccaaaat ccctatcata
    12961 catctcatta acatttttaa aatcgtcaat atttaataat actatgcata atttttcaat
    13021 cataggattt gaaattttat cttcaaatac actcataaat tcatgataat tcagtaactg
    13081 tgtcaatgaa tcaatcttat tttgtttacc taattgcgtc attattcctg caaaaatact
    13141 tggttctcca aattcatcac gcattagttg acctctacat cttaaccata tatattctcc
    13201 actacgattt ttagccctaa attccactgt atgataatcc atctggtttt tacctatttc
    13261 agtgtttgac ttataaaaac gattccagtc ttctggatgt acaatttttt tccagtaaac
    13321 aagcggattt tcaacaattt ctcctggtaa gtcaaataat tcaacttgtg atggtgaata
    13381 tcgaaatacg cctgttttca tattgcaaat gtatatatat tcatctgtac ctctaatcaa
    13441 agcatcataa agcaatgtag aattataatc aaaattgtct cttgaaaaat taaaaactcc
    13501 tttttcttct ttattatttg atatttcatg aaattcatta atcttaattg gtttatttaa
    13561 gcttgttgag tttgcaatat ctattgattg atgcatatga acaactgtgc catcttgcca
    13621 tgtaattaaa ctatcataat tttcaaaaac acgatttaac ttattacatt tttcatacca
    13681 ctttattaat actccttttt tatcattctt taataattct aaaactttac aaaaactaca
    13741 agtagaattt ttttcaggat ataatacttg ccaacaaact tttccttctg gatctaaaat
    13801 attatattct tctttcattt ttttgttcat aaaaataatt tcattagtat gaatatttgt
    13861 aacatatata ttaatctgta gttgatttaa tataacttca aaattatgtt tgttcatata
    13921 cttatctcct ccatagttac tgctctttat aacaacattg ttaaaattat tctttttaga
    13981 agatttactt ataacttata catatacact aaatacctta atttaattag aaagtttatt
    14041 acttcatatt ttaaattata attgataaaa aaaaatacag caatatagta ttaccaattt
    14101 aaaaaaaatt aatattacat aatacatctc tctcttatat taaattttaa cttggtacta
    14161 atcttgacaa attgtccaca aaaataaatt atagatttta ttattgtttt aaatttttat
    14221 aaaaaaatca gtattattga tactattgaa gttaaaaagc attaatttct aa
//
LOCUS       DAGSRM010000100        14202 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.100, whole genome shotgun sequence.
ACCESSION   DAGSRM010000100 DAGSRM010000000
VERSION     DAGSRM010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14202
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.100"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            541..1434
                     /locus_tag="MJE33_003062"
     CDS             541..1434
                     /locus_tag="MJE33_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HBY9648981.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELRNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     MLKLEEGDSHHHSNCGCGGNK"
     gene            1434..2828
                     /gene="gltA"
                     /locus_tag="MJE33_003063"
     CDS             1434..2828
                     /gene="gltA"
                     /locus_tag="MJE33_003063"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HBY9648982.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIIF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLDINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKAKVNA"
     gene            3194..5413
                     /locus_tag="MJE33_003064"
     CDS             3194..5413
                     /locus_tag="MJE33_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBY9648983.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIEASKRELENYLEVNISLLKALSQDDRFSNGETSLIEKGKLLSPYQKEYNLFMIGI
                     TDTKGNASSTYRENVNSIKDKPSFEKAIKTKQVVVSDIEVSNVTGDKVFIIYVPIVKN
                     NEMIGTIFASFYFQDVNNLISRSNFDDSIKFLMIDKDYTIISHPNKKYVNDKSKMLDL
                     EGNIIGTTKSEILKNINKKCQGDFLSWDNWRLYNVKYTNIKWTNWTLVSKCNIFKNFK
                     NLIVNFMIKLYFYIVIFMILWKLSNAKLIEQLKKLAYYDSLSGIKNKEKFRKDSMYIL
                     KNYYQDNFYLVQLDVNKFKYINEMFGYAEGNKILIHISQVLNNNTNKYEICARMDNDH
                     FILLIACNTEDELLNRLSKINKEICNLNTTNSSKYKIVMSSGIYKITKKDDIKKIDLL
                     IDRANIAAKSKKEKYEHSYSFFNEDTRNRLYKEKRLEDNMNKALEKGEFIVYYQPKYS
                     LDDVNEIEGAEALIRWNSPEFGFISPIDFVPLFEKNGFIVNIDMFVFEEVCKTLNKWI
                     NKGYTPVPISVNMSRVHLYRDNFIENITDLISKYNISPEFIELELTESVVFDNLNILI
                     DIMKKIKEIGFLISMDDFGSGYSSLNLLKDLSFDILKLDRGFLIETTDTKRGKIIISK
                     IVEMAKAIDIKVICEGVETYEQVEFLKEIGCDKVQGYLFAKPMVLDEFEKHLNFKFD"
     gene            5560..6306
                     /locus_tag="MJE33_003065"
     CDS             5560..6306
                     /locus_tag="MJE33_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9648984.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIEKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVVLDGYDVSKLKGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLEGASIKDMNIQTKSISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PAILLADEPTGNLDSKSTDDVLKLFTRINKEQKVTTLMVTHEAYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            6306..8186
                     /locus_tag="MJE33_003066"
     CDS             6306..8186
                     /locus_tag="MJE33_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9648985.1"
                     /translation="MDFRKFAYNNIVRNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     TKSGMQSGSTAFMALMTSELIIFGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTEFYFPVKAMLLTIVS
                     FMILFLITGPMSLKLLSKKSILELLVGTKKPKKEIKSSKLFGIIGVICLILGYAMILF
                     GNKLKINGGMYTTALLITLGIFLFFSQFTILILKYLKNKEKFYKNKVNMLLISNLIYK
                     LKDNTRLLFLITILLSATLVAFTTTSTLVSSQGEASKNNFPMVYSYFSEHNNEKEYEE
                     LQQIREAIKGYDYKELSFPVLKYNREVLLSVKNYNKLSKELGLDKISSKLEKYEGIII
                     PSSNSQEHINSLKQLKNYSIKNLNIEVKKSKNKIIFPTGLFSKVVVISDDLYNEVKGE
                     FSKINFHGFDYKNWQSSAKITENLKEKHFNLDREYNRSYFLTLPDMYLVELQQSKILQ
                     FMGVFIGVILFIAVFSFLYFRLYTDEIEDNVKYKKLSKIGLSFKNMNKIVSIEIGTLF
                     FIPYFIAIINSIISLLFLNQVLNNSFKLENLGILLLISSIYSLYFYLLKKNYTRKVWF
                     FD"
     gene            8305..9696
                     /locus_tag="MJE33_003067"
     CDS             8305..9696
                     /locus_tag="MJE33_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBY9648986.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAILTIGYYIS
                     GKSNLRFSKSNLKLDKKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLVVATIILCMGMV
                     VVMLFPEAIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            9803..10843
                     /locus_tag="MJE33_003068"
     CDS             9803..10843
                     /locus_tag="MJE33_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBY9648987.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISTDSSVNKVEKA
                     VEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDDSL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
     gene            11332..11985
                     /locus_tag="MJE33_003069"
     CDS             11332..11985
                     /locus_tag="MJE33_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBY9648988.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFINKFMEKNKDFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVNASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKASEKIDGIIDGMKF"
     gene            12061..12270
                     /locus_tag="MJE33_003070"
     CDS             12061..12270
                     /locus_tag="MJE33_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648989.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNIVMIDDGTGTLKQAVF
                     IGYKQIQINLNCSHCQNLNRIKLNL"
     gene            complement(12410..12976)
                     /locus_tag="MJE33_003071"
     CDS             complement(12410..12976)
                     /locus_tag="MJE33_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBY9648990.1"
                     /translation="MMNNELNTIILETLNNADITSNDIPSIDLYMDQIISLIDNKLSA
                     NKRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRI
                     LHGVYEKDNFSEKDLVSCYEKFMLIKENQRKNIPDFIESNFENISINPENKDDLLIAL
                     LSLTSMADQLKNISEKLVDRYFPDITKK"
     gene            13137..13790
                     /locus_tag="MJE33_003072"
     CDS             13137..13790
                     /locus_tag="MJE33_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBY9648991.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVTLQETSLL
                     MIVSVSVFGLSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
     gene            complement(13948..14124)
                     /locus_tag="MJE33_003073"
     CDS             complement(13948..14124)
                     /locus_tag="MJE33_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9648992.1"
                     /translation="MYKYDDYVNLTNDLDLPVEIRNKCVRIVSVSENLEDIIIVHKFK
                     LYVVNEKYIKGIME"
BASE COUNT         5573 a         1400 c         2383 g         4846 t
ORIGIN      
        1 acataagtaa ataaatcttt agaaattgta aaaattttat ataaaaattc aaataattaa
       61 tgaatattga aaattttaga tatataataa aataagagta tattttatac aatatgaaag
      121 ctggtattgc aatagattaa agccttatac acgtttagtt tttaaagaaa ttagtgttat
      181 tatgaaaacc taagttaatt tattaaataa ataattgaat aaaaaaattt tattatgtat
      241 ttataaaatt tttgttacaa atttggcata atatagtata ctttaagtgt gtattaggtt
      301 aaatctataa caaattagac gtatgatata gacttagtgt ttattggcat ttacaagaaa
      361 tttgttaata tcaactattt gtagtttggg cagttataat tattataggg gggatgtcca
      421 atacaaaaca atcaaccaag aactaatatt atattataaa gagttatagt tttgtgtgtt
      481 tatgttttta attaaattta aagtgggaaa aatatatttg ttagaatgga gaatagcgag
      541 atgagtaata aaatagttag taagagacaa ttaacagata gtatttattt aatggaaata
      601 gaggcaccta gagttgcaaa atcttctcaa ccaggtcaat ttataataat taaaaatgat
      661 gaaaaaggag agaggattcc tcttacaata gctgactatg atagagaaaa aggaacagta
      721 actattgtat tccaaacagt tggtgcttca actaaaaagt tagcaatgtt tgaagaaaac
      781 gattttgtaa tggattttgt agggccatta ggacaagcaa gtgaatttat acatgaagat
      841 atagaagaat taagaaataa aaagatatta tttgtagctg gtggagttgg ttctgctcca
      901 gtttatccac aagttaaatg gtttaaagag catggattag atgtagatgt aataattggt
      961 gcaagaacta aagaattaat tatattagaa gatgatatga aaaaagtagc taaaaatgta
     1021 tatgtatcaa cagatgatgg tacatatgga tttaatggaa gagttacaga cctattaaaa
     1081 gatttagttg ataatcaagg taaaaaatac gaccaagcaa tagtaattgg accaatgata
     1141 atgatgaaat ttatgtgcca attaactaaa gaactaaata tacctactat agtaagttta
     1201 aatacaataa tgatagatgg aacaggtatg tgtggtggtt gtagagttag tgttggaaat
     1261 gaaactaagt ttgcatgtgt agatggtcct gaatttgatg gtcatttagt tgattttgat
     1321 caagcaatga gaagacagtc tatgtacaag actcaagagg gaagagctat gctaaaactt
     1381 gaagaaggcg atagtcatca tcatagtaat tgtggatgtg gaggtaacaa ataatggatg
     1441 ctaaaaaagt aaaagtacca gttagagaac aagaacctgc agtgagagca acaaattttg
     1501 atgaggtatg cttagggtat aataaagaag aagctatggc agaagctaat agatgtttag
     1561 cttgtaaaaa accaaaatgt gtgggtggat gtccagtagg aatcgacata ccagggttta
     1621 taacaaaaat taaagaagat gatatagaag gtgctgctaa agtaatagct aagagcagtt
     1681 cattaccagc tgtttgtggt agagtatgcc cacaagaaag tcaatgtgaa ggtgtatgta
     1741 tacttggaat aaaatcagat gctgtatcaa taggtaaatt ggaaagattt gtagcagatt
     1801 ggtcaaaaga aaatgacata aatttatctg atacagagcc taagaaaaat caaaaagtag
     1861 cagtaatagg aagtggtcca gcaggtctag cttgtgctgg tgatttagct aaaaaaggct
     1921 atgatgtaac aatctttgaa gcaatgcatg agccaggtgg agttttaact tacggaatac
     1981 ctgagtttag acttccaaaa caagctgttg ttcaacctga aatagacaat ataagaaaat
     2041 taggtgtaaa aatagaaact aatgtaattg ttggtaaaac aattacagta gatgaactta
     2101 tagaagatga aggatttgaa gctatattca taggttcagg agctggtctt ccaatgttta
     2161 tgaatatacc aggggaaaat gcaaatggag tattttctgc aaatgagttt ttaactagag
     2221 taaacttaat gaaggcttat agagatgatt atgatacacc tattagttct ggtaaaaaag
     2281 ttgctgtagt aggtggtgga aatgttgcta tggacgctgc tagaactgca ttaagattag
     2341 gttcagaatc atatatagtt tatagaagaa gtgaaaaaga gcttccagca agagctgaag
     2401 aagttcatca tgcaaaagaa gaaggcatta tatttaatac attaacaaat cctaaggaaa
     2461 ttttagtaga tgaaaatgga tatgttaaag gtatggtttg tataagaatg gaattaggag
     2521 agccagatga ttctggaaga agaagaccta tagaaataga aggttctgaa tttgtacttg
     2581 atgtagacac agttataatg tcacttggaa caagcccaaa tccactaata tcttctacaa
     2641 caaagagtct tgatataaat aagaagagat gcttaataac agatgaaaat ggtcagactt
     2701 ctaaagaggg tgtatttgca ggtggagatg ctgttactgg agcagcaacg gtaatatctg
     2761 caatgggtgc tggtaaaaca gcagcagcat caatagatga atatttaaaa gcaaaagtta
     2821 atgcttaatt tgtaaattaa agagagggtg tctcaaaatg aactttttag ttcatgagac
     2881 actctttttg tacaaaatca aatatgtttt aatcatttca acaatgaaaa tttcattcct
     2941 gtagaaagtc taacttttag gtcttagtac aatctctatt ttgatgtagc cactttttta
     3001 gatggtttaa taatttatca gttattattg taatatttga tactaagtaa cataactttg
     3061 tctaactatg ataaaatagt tatacaataa aggtcttgta aggagtgaat aatgtgtggc
     3121 taataaaatc agatttgtca aatctaacat taaataatag aaaaataaat tgtgtaatta
     3181 atggtatttt aatatgaaaa aaagagaaaa agtatttgat gggctagttt ttaagcatat
     3241 attaatctta ttaatactat tagttttgat tgatttaaca agtaccatca tatatgcaag
     3301 taactctatg gaaattactt ctagaaatat gattgaggca tcaaaaagag aacttgaaaa
     3361 ctatttagaa gtaaacatat ctttattaaa agctcttagt caagatgata gattttctaa
     3421 tggtgaaaca tcactaattg aaaagggaaa attattaagt ccatatcaaa aggaatataa
     3481 tctttttatg attggtataa cagatacaaa aggaaatgct tcaagtacat atagggaaaa
     3541 tgttaactca ataaaagata agcctagttt tgagaaagca attaaaacaa agcaagtagt
     3601 agtttcagat attgaagtca gtaatgtaac aggtgacaaa gtatttatca tatatgtacc
     3661 aattgtaaaa aataatgaga tgattgggac catttttgct tctttttatt ttcaagatgt
     3721 aaataattta ataagtagaa gcaattttga tgattctatt aaatttctaa tgatagataa
     3781 agattatact attatttctc atccaaacaa aaaatatgta aatgataaaa gtaagatgct
     3841 tgatttagaa gggaatatta taggaaccac taaaagtgaa atcttaaaaa atataaataa
     3901 aaaatgtcaa ggtgattttt tatcttggga taattggaga ttatataatg taaaatatac
     3961 gaacataaag tggactaatt ggactttagt atcaaaatgt aatatattta agaactttaa
     4021 aaatctaata gttaatttta tgataaagtt gtatttttat atagtaatat ttatgatatt
     4081 gtggaaatta agtaatgcta aattaataga acaacttaaa aaattggctt attatgattc
     4141 tttaagtgga ataaaaaata aagaaaagtt tagaaaagat tctatgtata ttttaaagaa
     4201 ttattatcaa gataattttt acttagtaca attggatgtc aataaattta aatatataaa
     4261 tgagatgttt ggatatgctg aagggaataa gatattaatt catatatcac aggttttaaa
     4321 taataataca aataaatatg aaatttgtgc taggatggat aatgaccatt ttatattgtt
     4381 gattgcttgt aacactgaag atgaactttt aaatagatta tctaaaataa ataaagaaat
     4441 ttgtaactta aatactacta actcatctaa gtataaaatt gttatgtcaa gtggaatata
     4501 taagattact aaaaaggatg atattaaaaa gattgacttg ttaattgata gagcaaatat
     4561 tgcagcaaaa agtaaaaagg aaaaatatga acattcatat tcatttttta atgaagatac
     4621 aagaaatcga ttgtacaagg aaaaacgatt ggaagataat atgaataaag ctttagaaaa
     4681 aggtgaattt atagtatatt atcaacctaa gtatagttta gatgatgtaa atgaaattga
     4741 aggagcagaa gccttgataa gatggaatag tccagaattt gggtttattt cacctataga
     4801 ttttgtacct ttatttgaaa agaatggttt tattgtaaat attgatatgt tcgtgtttga
     4861 agaagtatgc aaaacattaa acaagtggat aaataaaggg tatactcctg taccaatctc
     4921 tgtaaatatg tctagagttc atttatatag ggataatttt atagaaaata taactgattt
     4981 gataagtaaa tacaatatat ctcctgagtt tattgagctt gaactaacag aaagtgttgt
     5041 gtttgataat ttaaatatat tgattgatat aatgaaaaag attaaagaga taggattcct
     5101 tatatcaatg gatgattttg gttcgggata ttcatcttta aatctgttaa aagacctttc
     5161 ttttgatatt ttaaaattag atagaggatt tttaatcgaa acaacagata caaaacgtgg
     5221 aaaaattatt atttctaaga ttgtggaaat ggcaaaagcg attgatatta aagtaatctg
     5281 tgagggcgta gaaacttatg aacaagtaga gttcttgaaa gagataggtt gtgataaggt
     5341 tcaaggatac ttatttgcta aacctatggt attagatgaa tttgaaaaac acttaaactt
     5401 taaatttgat taatcattaa taattattag actataagat accctaacaa cttacttaca
     5461 aatatgtaag ttaattgtta gggtattttt atggttaaag tagtatattc acgatattat
     5521 ggtattgtag taaaaataaa aaattatgga gaatataaaa tgaacatatt gagtattaga
     5581 aatatatcaa aaacatattg tggaaatatt ccatttaaag cattagataa ggttagtcta
     5641 aatatagaaa agggagaatt tgtgtctgtt atgggaccat ctggaagtgg taaatctact
     5701 cttttaaata taatatctac agttgataga cagagtgaag gtgaggttgt attagatgga
     5761 tatgatgtaa gtaaattgaa aggagaaaaa cttgctgaat ttagaaggaa acaacttggt
     5821 tttgtatttc aggattttaa tttgattgac actttaactg ttggcgaaaa tattatgttg
     5881 ccacttacac tagagggggc aagtatcaaa gatatgaata tacaaactaa aagtatatct
     5941 aaatttttag gaatagataa aattttagat agaaaaacct atgaaatttc aggaggtcaa
     6001 gctcaaaggt gtgctattgc tagagctatt ataaataaac ctgccatact tcttgcagat
     6061 gagccaactg gaaatcttga ttcaaaatct actgatgatg ttttgaaatt atttactagg
     6121 ataaacaaag aacaaaaggt aacaacactt atggtaactc atgaagcata tagtgcaagt
     6181 tattcagata gagtaatttt tataaaagat ggttgtatat atacagaaat aaaaaaatct
     6241 gaaagcagta attctttcta ttctgatata ctagctgttt tgtctcaaat aggaggtgta
     6301 agatagtgga ttttagaaag tttgcttata ataatatagt tagaaatttc agggcatatt
     6361 tagcatattt tttaagttct gttttttcta taatgatatt ttttgttttt gcagtgtcta
     6421 tgttccatcc aattataaca aaatctggaa tgcagagtgg ttctactgca tttatggctc
     6481 ttatgacttc agaattaata atattcggtt ttagtctact ttttatattg tactctttag
     6541 gaaatttctt aaaatataga ttgaaagatt ttggagtatt gatgataatt ggaatgagta
     6601 acagacaact gaaaaaattg atattaattg aaaatttgat aataggattt ttagcagtga
     6661 tgttaggaat attacttggt ttatcaattt caaaattgtt tttattgtat ttaagcaaac
     6721 tgttttatat gaatttaact gaattttatt ttccagtaaa agcaatgcta ttgactattg
     6781 tatcatttat gattttattt ttgataacag gaccaatgag cttaaaatta ttgagtaaaa
     6841 agagcatttt agagctttta gtaggtacaa aaaagcctaa aaaagagata aaatcttcaa
     6901 agctatttgg aataattggt gtaatatgtc ttatattagg atatgctatg atactatttg
     6961 gcaataagtt aaaaataaat ggaggtatgt atacaacagc actattaatt actttgggga
     7021 tatttttatt tttttctcaa tttactattt tgatattaaa gtatttaaaa aataaagaaa
     7081 aattttataa gaacaaagta aatatgttac ttataagtaa tttgatatat aaattaaagg
     7141 ataatacacg attgttattt ttaataacaa tacttttatc ggctacttta gtagcattta
     7201 caacaacttc aacacttgta agttctcaag gagaagcttc taaaaataat ttcccaatgg
     7261 tttactcata tttttcagag cataataatg aaaaagaata tgaagaacta caacaaatta
     7321 gagaagcaat caaaggatat gattataagg agttaagttt tcctgtactt aaatataata
     7381 gggaagtctt actaagtgta aagaattata acaaattatc aaaggaattg ggattagata
     7441 aaattagttc aaagttagaa aaatatgaag ggattataat tcctagtagc aattcgcaag
     7501 aacatataaa ttctttaaaa caattaaaaa attatagtat aaaaaattta aatatagaag
     7561 taaaaaaatc aaaaaataaa ataatttttc ctacaggact gtttagtaaa gttgtggtta
     7621 taagtgatga cttatacaat gaagtaaaag gtgaattttc aaaaattaat tttcatggat
     7681 ttgattataa aaactggcaa agcagtgcaa agataactga aaatttaaaa gaaaaacatt
     7741 ttaatttgga tagagaatat aacaggtcat attttttaac attaccagat atgtatttag
     7801 tagagttaca acaaagtaaa attcttcaat ttatgggtgt ttttatagga gtgatattat
     7861 ttatagcagt ttttagtttt ttatacttca gattatatac agacgagata gaagataatg
     7921 taaagtacaa aaaactatca aaaataggtc tatcttttaa aaatatgaat aagattgtta
     7981 gtatagagat aggtacattg ttttttatac cgtattttat agcaattata aatagcataa
     8041 tttctttact gtttttaaat caagtattaa ataatagttt taagttagaa aatttgggta
     8101 tattactact gatatcatct atatattcac tttattttta cttattaaaa aaaaattata
     8161 ctagaaaagt ttggtttttt gattaaagtg gtatataata gatatataaa ttaatattta
     8221 aaaagtagtt aatattaggg gagggcattt gcacctccct tgtaatatgt ggaaaattga
     8281 atattagaag aaggaggaga cttgatggaa aatcaacaat tattaggaac agaacgaatt
     8341 agtaaattac tactaaaata ttctatacca gctataatag gtatgttggt aaatagttta
     8401 tataatgtag tagatagaat ttttattgga aatataccag gggttggacc acttgcaata
     8461 acaggtcttg gtgtaacaat gccaataatg acaataatac ttgcatttgg gatgttgata
     8521 ggtataggaa ctactactac catatcaata aaacttgggc aaggtaaagt agaagaggca
     8581 agaaaactta taggaaatgc aatgacgtta tctgtaataa caggaattat cataatgata
     8641 cttggaatat tatttgcaaa taaaatactt acattgtttg gagcaagtga aaatacttta
     8701 atatatgcaa aatcatatat caatattata ttattaggta cagtagtaaa tcttctttcc
     8761 ttttctttaa accactcaat aagagctgat ggtagtccta aaatttcagc aggaataatg
     8821 atagtaggat gtttaactaa tatagtttta gactggatat taatatttgg attcaatttg
     8881 ggaatacaag gagctgcaat tgctacagta acttctcagg ctcttacagc tattttaact
     8941 atagggtatt atataagtgg aaaatcaaac ttacgattca gtaaatctaa tctaaaatta
     9001 gataaaaagc taataaaagc agtgtttgca ataggtatgt cgccatttgc tatgcaactt
     9061 gcagcaagtt tagttcaagt aatatcaaat attgcactta aaactcatgg tggagattta
     9121 gctatagggg ctatggctac tatttcttct attgcaatgg tatttttgat gcctatcttt
     9181 ggaataaatc aaggagcgca acctattata ggtttcaatt atggagctga aaaatatgat
     9241 agggttaaaa aagcatattt aggttcatta gtagtagcta ctataatttt atgtatgggt
     9301 atggtagtag ttatgctttt cccagaagct attataggta tatttaataa agaccctgaa
     9361 cttatgaata tatctgttaa tggattaaga atatacttac ttatgttgcc aattgtaggc
     9421 ttatcagtta caggaacaaa ctttatacaa tctataggaa aagcaaaaat ggctatgtta
     9481 ttgagccttt taagacaagt aattttattg ataccagcag tattaattct tcctacattc
     9541 ttaggtttac agggtgtatg gacagctcaa ccagtgtctg attttatagc tacagtaatt
     9601 acaggtatag ttgtatttag agaattaaaa agatatactc ctaaaactga aaaattaaat
     9661 gaaaatgaaa gactaaacga gataactact gagtaaaaat ataaattcag ataattgagc
     9721 aagaaattgc aaaaaatacg attatatgat aaaataaaac cgctgtcaag gataagagaa
     9781 attctaatta tggagtgatt ttatggaatt tacaaagaaa agcaaattaa aaaatatgta
     9841 tgtaaacatg aatgtagcta gaatgttgtc taagataaat gagtataagg gtagacaact
     9901 gctttacaaa aaacaaccaa aagaaatttt agaaaactta gaaaaaaaat ctttagtaga
     9961 ttgttctgaa tctacataca ttggaaatca aagagatagt agtaatttta atctagaaaa
    10021 gttgataagt aatgaagtta cacctagaag tagagaggaa ttgagtattg ttgaatacag
    10081 agatgtggta aaaactatta acagtgctta tgagagtatt cccatcagtt cacaaacaat
    10141 tcttgagctt catggatact tatataaatt ttcatctact agaggaggaa gttataagtc
    10201 agataatgat tttattgaac acaatttaaa acctagcgaa tttataagta ctgattcaag
    10261 tgtcaataaa gttgaaaaag ctgtagaaga aatatgtgaa gcttataata cattgataga
    10321 ggaagatgaa attgatatat taatattaat ttctgcattt gtgttagatt tcatattgat
    10381 acatccattc aaagaaggta atataaaaat ggcgagagta cttatcctat tgttactaaa
    10441 taaaaatggg tatgaagttg gaagatatat aagtttagga aagatatttg atgatagttc
    10501 atatgaatat tatagtaatt taaattattt gaaagcctca ataggtagtg aaaaagcaga
    10561 tatgaatgca tggattgagt attttttaga gacgatatta actgcatatg agaagctgga
    10621 tgatagttta aatatatctg ataagaaaag acaaacaaaa actagtagaa tagagaaaat
    10681 aataaattct acccttggat attttacaaa agaagatata agagatttat gtcctgacat
    10741 tccagaacct actataaata gagtttttaa taatctaaga aaacaagata agatagaagt
    10801 tgttgcaaga ggaagaagtg caaaatggaa gaaaaaatat taaaaaatgt ttagataact
    10861 aaaatggtat agtgttttac actataccat ttttaatgtc attagcatga gcaataattt
    10921 aacaacttaa gcaatatcaa ttttatttat tgattatttt tgaactgaaa aatggcgtta
    10981 tatggtatac ctcagtggta attaattttg gatgagaaga tataatatta cagaatatat
    11041 taggagtata tgtagaaaaa atataataaa agtgtgaggc ttagtaatta acaaattatg
    11101 tggcttcaca cttttttata aaataaagat acttagcaga ataatatttt agttgatatg
    11161 tttataagat ggtacttgtt tgggtaaaag tataaatagt aaaaaagtag aagtaatgtg
    11221 tataaaaaca taagttacat tctgtaataa tagttatttt ataattcaac aaaagaatag
    11281 aatgggtata tttgtgaaat aataatataa aataatctta ggagtgagct tatgaagaaa
    11341 aaattattat atataaatgt aaattcaaaa cctgaagact tatcttcaag taaaactgta
    11401 gcaagaaaat ttataaataa atttatggaa aaaaataaag attttgaggt tgaagagata
    11461 gacctttata aagaacatat accaagactt gagtatcagt actttgaaaa aagaaatagc
    11521 atagttagtg aagaaaatgc taaaaattta gacgataagg atagaaaaga gcttacaaag
    11581 attagaaatc tttgtgacca atttgtgaat gcaagtgttt atgtaatagc agcacctatg
    11641 tggagtttat cttttccagc accacttaaa gagtatatag attgtataat tcaagatgga
    11701 aaaactatat cttttgaagg caatgataaa ccacaaggaa ttttaaatga tattgataga
    11761 agtatggtat atatacaatc ttctggtgga cacataccat gggtgttgaa gccagttatg
    11821 aataaagggt taaattatgt ggaaagtatt atgaaattta taggaattaa aaaatttgat
    11881 gaattgttag tagatggaac tggaactagt gaagaagaaa gacaagctgc aatagaaaaa
    11941 gcatctgaaa aaatagatgg tataatagat gggatgaaat tttaattata atattttatt
    12001 aaaaatataa aggaaaaacc tcttttaatg tagaaactta tattgaagga ggcgagaatc
    12061 atgcgagaag aaaaaagtaa tgaaaagtat gattgttatt ggtgtaatca agagaataat
    12121 ttctgtgtag aaataaaaga taatatagtc atgatagatg atggcactgg tacgttaaaa
    12181 caagccgttt tcatagggta taaacaaatc caaattaatt taaactgttc acattgtcaa
    12241 aacttaaata gaataaaatt aaatttgtag aattttaaaa tggaaattat tgaaggaatt
    12301 aaaaatagaa agtgttaata tttagactga aaattatatt ttatacaata aaagtttatt
    12361 taaataacat tgtattttaa tatttatttt cagtcttact ttattaaaat tattttttgg
    12421 ttatgtctgg aaaatatctg tctactaatt tttctgaaat attttttaat tggtcagcca
    12481 ttgaagtcag acttaataag gctatcaata aatcatcttt attttctgga tttatactaa
    12541 tattttcaaa attagactct ataaagtccg gtatattctt tctttgattt tcttttatta
    12601 acatgaattt ttcgtaacat gaaactaaat ctttttcact gaaattatcc ttttcataca
    12661 caccatgtaa gattctttta atttcttgta ttgtaagtgt atttttcata gaataaataa
    12721 ttatcatctg taaaatttgt tctttagtat acttttttcc cttaactggt ttgattaatc
    12781 cctctttgct gtaattattt atcatggttt tagtaagaat tttatcactt tcaaatcttt
    12841 tattagcaga aagcttattg tcaattagag aaataatttg atccatatat aagtcaatag
    12901 atggaatatc gtttgaagtt atatctgcat tatttagtgt ttctagtatt atagtattaa
    12961 gttcattatt catcatatgt tacctccata atatatataa tacactaaat tagaaagtaa
    13021 aacaatatta aggcttgtaa aaaaaacata aagtggtata attaaatata taatatagta
    13081 atcaaaacta tatttatgaa tctgattttt aaaatacata attgtaggag gacgaaatgt
    13141 atcaattttt tttaaagggt agagacccaa taagcagttt aactcatttt ataggtgctt
    13201 gtttatctct tttagcaact ataattttag tttttcaatc tgttacatta caggaaactt
    13261 ctctcttgat gatagtatct gtatctgtat ttggactttc actaattgct ttatatagtg
    13321 caagttcgta ttatcatttt ttaaagggga caccagaaca agaattgttt tttagaaaag
    13381 tagaccatgc aatgatttac gtattgattg ctggctctta tacaccaata tgtctgaatt
    13441 ttatggaaaa aaaagaaggc ataatatttg ttacagcaat atggatagtt gcatttattg
    13501 gcataataat aaaaatattt tggatggatg cacctagatg gttatctacc agtatatatt
    13561 tacttatggg gtgggcaatt gtttttgata taaatgcatt caattcaatt cctaaggatt
    13621 gccttagact attaattatg gaaggtgtat cttactctat aggtgcgatt atttatataa
    13681 ttaaaaagcc taatataagt cctgagtttg gatttcatga aatatttcat atttttatta
    13741 tgataggttc tttatttcac tttttagcag ttttattgta tgttttatag ttttaaaaga
    13801 tttgattaat tgatactaaa attctacaat gaataaaatg gaactgcctt atatagatta
    13861 tatcttagac aataagacag ttccttatta taaatattaa attctactta taataaaatt
    13921 ttgataaaaa tagtccattc aaaatgccta ttccataatt cctttaatgt acttttcatt
    13981 tacgacatat agtttgaatt tatgtactat aattatatcc tctaaattct ctgaaacact
    14041 aacaattctg acacatttgt ttctaatttc tacaggtaaa tctaaatcat ttgttaaatt
    14101 cacatagtca tcatatttat acataaaatc atccctttac attaagatat acttattata
    14161 ctatatttta ataagttttt ttaaattatt acaatttttc cc
//
LOCUS       DAGSRM010000101        14044 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.101, whole genome shotgun sequence.
ACCESSION   DAGSRM010000101 DAGSRM010000000
VERSION     DAGSRM010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14044
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.101"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            482..1681
                     /gene="hadA"
                     /locus_tag="MJE33_003074"
     CDS             482..1681
                     /gene="hadA"
                     /locus_tag="MJE33_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HBY9648993.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            1720..2520
                     /gene="hadI"
                     /locus_tag="MJE33_003075"
     CDS             1720..2520
                     /gene="hadI"
                     /locus_tag="MJE33_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HBY9648994.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            2535..3761
                     /gene="hadB"
                     /locus_tag="MJE33_003076"
     CDS             2535..3761
                     /gene="hadB"
                     /locus_tag="MJE33_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HBY9648995.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            3761..4888
                     /gene="hadC"
                     /locus_tag="MJE33_003077"
     CDS             3761..4888
                     /gene="hadC"
                     /locus_tag="MJE33_003077"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HBY9648996.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKSETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            4936..6069
                     /locus_tag="MJE33_003078"
     CDS             4936..6069
                     /locus_tag="MJE33_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBY9648997.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            6102..6887
                     /locus_tag="MJE33_003079"
     CDS             6102..6887
                     /locus_tag="MJE33_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBY9648998.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            6906..7943
                     /locus_tag="MJE33_003080"
     CDS             6906..7943
                     /locus_tag="MJE33_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBY9648999.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            8426..10057
                     /locus_tag="MJE33_003081"
     CDS             8426..10057
                     /locus_tag="MJE33_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY9649000.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(10192..11118)
                     /gene="fba"
                     /locus_tag="MJE33_003082"
     CDS             complement(10192..11118)
                     /gene="fba"
                     /locus_tag="MJE33_003082"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HBY9649001.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            11448..11777
                     /locus_tag="MJE33_003083"
     CDS             11448..11777
                     /locus_tag="MJE33_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HBY9649002.1"
                     /translation="MPQIKIRGINENDICKISEKMINDLVEAVKCPRDYFEIECIKSV
                     AIRDGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESIDLAFTVFEKEKY
                     YENGEHF"
     gene            11777..12499
                     /locus_tag="MJE33_003084"
     CDS             11777..12499
                     /locus_tag="MJE33_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBY9649003.1"
                     /translation="MAKKQRIDKILSNLGYGSRSEIKKYCKQGSVVVNGSEVSNPGTQ
                     VDTENDEILFNGEEVIYREYIYLMMNKPDGYISATTDKYDPTVLDLIDLSYLAFEPFP
                     VGRLDKDTEGLLVLTNDGKLSHRVLSPKKHVPKTYYAKIDGVVTEEDVEAFLEGVVLD
                     DGYKTMPSQLNILKSDDESEIELTIHEGKFHQVKRMFESVGKKVVYLKRLSMGNLKLD
                     ESLELGEYRELTDEEVKLIEER"
     gene            12526..13887
                     /gene="rlmD"
                     /locus_tag="MJE33_003085"
     CDS             12526..13887
                     /gene="rlmD"
                     /locus_tag="MJE33_003085"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBY9649004.1"
                     /translation="MSVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRA
                     GVKKVRSKKAEVKMIELLENSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMD
                     LFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
                     ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIMVNIVTS
                     SQEDFDMYEFKDMLLGIDLKAELVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILG
                     LKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKV
                     YGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKD
                     AIRDICGFGAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHCETVVLL
                     RRK"
BASE COUNT         5525 a         1634 c         2715 g         4170 t
ORIGIN      
        1 aattgttatt ttttagatta aatattatta aaaaattatc taagtttgaa attagttaac
       61 taacttgctg attaattaca atgataacat gtgtttttga gtttagcaat gaaaactttt
      121 tttgaaatgt caagcttttt cataaaaaaa tatataaaaa aaacttttat aagcatagaa
      181 agaatagcaa aatgcaatgg atagtggcaa gatgccacga tatgaaaaag attattcaat
      241 tgcatattgt taaaaaacat tcaaatcaag cgatattttg attaaatata aagttggcac
      301 gataaatgct taacaatata ggtgtaagac aataaaacaa taaaaaataa ttttaataat
      361 ttttaagtta ttttagataa gattaggaac tttgacatca gacagttaat atcagaaaaa
      421 agtattttat gagtgattta atcgaaattt tataattgat actattaagg aggaaaacgt
      481 tatgctttta gaaggagtta aagtagtaga actttcaagt ttcatcgcag caccatgttg
      541 tgcaaaaatg ttaggtgact ggggtgcaga ggttattaag attgaaccta tagaaggtga
      601 tggaataaga gttatgggtg gaacatttaa atctccagca tcagatgatg aaaaccctat
      661 gtttgaatta gaaaatggaa ataaaaaggg tgtaagtatt aatgtaaaat caaaagaagg
      721 agtagaaata ttacataaat tattatcaga agcagacata tttgtaacta atgttagagt
      781 tcaagcatta gaaaaaatgg gtatagctta tgaccaaata aaagataagt atccaggatt
      841 aatattctct caaatattag gatatggtga aaaaggacct ttaaaagata aaccaggatt
      901 tgactatact gcatacttcg caagaggagg agttagccaa tctgttatgg aaaaaggaac
      961 atctccagca aatacagcag caggatttgg tgaccactat gcaggtctag cactagcagc
     1021 aggaagttta gcagcattac ataaaaaagc tcaaactggt aaaggtgaga gagtaacagt
     1081 aagtcttttc catacagcta tatatggaat gggaacaatg ataacaacag cacaatacgg
     1141 aaatgaaatg cctttatcaa gagaaaatcc aaacagccca ttaatgacta catataaatg
     1201 taaagatgga agatggattc aattagcttt aatacaatac aacaagtggt taggcaaatt
     1261 ctgtaaggtt ataaatagag aatatatatt agaagatgat agatataata acatagattc
     1321 aatggttaat catgttgaag atttagttaa gatagttgga gaagctatgt tagaaaaaac
     1381 attagacgag tggtcagctt tattagaaga agcagactta ccatttgaaa aaattcaaag
     1441 ctgtgaagat ttattagatg acgaacaagc ttgggcaaat gacttcttat ttaagaaaac
     1501 atacgatagc ggaaatacag gtgtcttagt taatactcca gttatgttta gaaatgaagg
     1561 aattaaagaa tatacaccag caccaaaagt aggtcaacat actgtagaag tattaaaatc
     1621 tttaggctac gatgaagaga aaataaataa ctttaaagat agtaaagttg taagatatta
     1681 attagtaatt ctagattttt taaaaggaga atgctaaaga tgtacacaat gggattagat
     1741 ataggttcaa ctgcatcaaa gggagtaatc ttaaagaatg gggaagatat tgtagcttct
     1801 gaaacaatat cctctggtac tgggactact ggaccatcaa gagttttaga aaaattatat
     1861 ggcaagacag gtcttgcaag agaagatatt aaaaaagttg tagttacagg atatggaaga
     1921 atgaactatt cagatgctga taagcaaata agtgaattaa gctgtcatgc tagaggggta
     1981 aatttcataa ttccagagac aagaaccatt attgacatag gtggtcaaga tgcaaaggta
     2041 ttaaaattag ataataatgg aagactatta aactttctta tgaatgacaa atgtgctgca
     2101 ggtacaggaa gatttttaga tgtaatggca aaaataatag aggttgatgt atctgaactc
     2161 ggaagtatat ctatgaattc tcaaaatgaa gtatcaataa gcagtacatg tacagtattt
     2221 gcagagtctg aggttatatc acatttatct gaaaatgcaa aaattgaaga tatagtggca
     2281 ggtattcata cttcagtagc aaagagagtt tctagcctag taaaaagaat aggagtacaa
     2341 agaaatgtag ttatggttgg tggagttgct agaaatagtg gtattgtaag agctatggca
     2401 agagaaatca acacagaaat tattgtacct gatatacctc aattaactgg tgctttagga
     2461 gcagcgttat atgcttttga tgaagcaaaa gaatcacaaa aagaagtgaa aaatatataa
     2521 aaagagggat gaaaatgtct gaaaaaaaag aagctagagt agtaattaat gatttattag
     2581 ctgaacaata tgcaaatgca tttaaagcta aagaagaagg aagacctgta ggttggtcaa
     2641 catcagtatt tcctcaagag ttagcagaag tatttgactt aaacgtatta tatccagaaa
     2701 accaagcagc tggagtagca gctaaaaaag gttctttaga attatgtgaa atagctgaat
     2761 ctaaaggata ttctattgac ctatgtgcat atgcaagaac aaattttggt cttttagaaa
     2821 atggtggatg tgaagctttg gatatgccag ctccagattt cctactttgc tgtaacaata
     2881 tatgtaacca agttataaaa tggtatgaaa atatttcaag agaattagat atacctttaa
     2941 taatgattga tacaactttc aataatgaag acgaagttac tcaatcaaga atagattata
     3001 ttaaagctca atttgaagaa gctataaaac aactagaaat tatatcagga aagaaatttg
     3061 accctaagaa gtttgaagaa gtaatgaaaa tatcagctga aaacggaaga ctatggaagt
     3121 attctatgag tttaccagca gattcttctc cttctccaat gaatggattt gacttattta
     3181 cttacatggc tgtaatagtt tgtgctagag gtaaaaaaga aactacagaa gcatttaagt
     3241 tacttataga agaattagag gacaacatga aaactggtaa atcttctttc agaggggaag
     3301 aaaaatacag aataatgatg gaaggtatac cttgttggcc atatatagga tacaagatga
     3361 aaacattagc taaatttgga gttaacatga caggtagtgt ttacccacat gcttgggcat
     3421 tacaatatga agttaatgat ttagatggaa tggcagtagc atatagtact atgtttaaca
     3481 atgtaaacct agaccgtatg acaaaatata gagttgattc tttagtagag ggtaaatgtg
     3541 atggagcatt ctatcatatg aacagaagct gtaaacttat gagtttaata caatatgaaa
     3601 tgcaaagaag agcagctgaa gaaactggat taccatatgc tggatttgat ggtgaccaag
     3661 cagaccctag agctttcact aatgctcaat ttgaaacaag aattcaaggt ttagttgaag
     3721 taatggaaga aagaaaaaaa cttaatagag gtgagatata atggaagcta ttttatctaa
     3781 aatgaaagaa gtagttgaaa atccaaatgc ggctgtaaaa aaatataaaa gtgaaactgg
     3841 taaaaaagct ataggttgtt tcccagttta ttgcccagaa gaaattatac atgcagctgg
     3901 aatgcttcca gttggtatat ggggagggca aacagaatta gatttagcta aacaatattt
     3961 ccctgcattt gcatgttcaa taatgcaatc atgtttagaa tatggattaa aaggtgctta
     4021 tgatgaatta tctggagtta ttataccagg tatgtgtgat acactaattt gtttaggaca
     4081 aaactggaaa tcagcagtac ctcatataaa atatatatca ttagtacacc cacaaaatag
     4141 aaaacttgaa gctggtgtaa aatacttaat cagtgagtac aaaggcgtaa aaagagaact
     4201 tgaagaaatt tgtggatatg aaatagaaga agcaaaaatt catgaaagta tagaagttta
     4261 caatgaacat agaaaaacta tgagagactt tgttgaagta gcttataaac attctaatac
     4321 tataaaacca tcaataagaa gcttagtaat taagagtggg ttctttatga gaaaagaaga
     4381 acatactgag ctagtgaaag atttaatagc aaaattaaat gctatgccag aagaagtctg
     4441 ttctggaaag aaagttttat taacaggtat attagctgat tctaaagata tattagacat
     4501 tttagaagac aacaatatat cagttgtagc tgacgactta gcacaagaaa caagacaatt
     4561 cagaacagat gtaccagcag gtgatgatgc gttagaaaga ttagcaagac aatggtcaaa
     4621 catagaagga tgttcattag cttatgaccc taagaaaaaa cgtgggtcac ttatagtaga
     4681 tgaagttaaa aagaaagata tagatggtgt tatcttctgt atgatgaaat tctgtgaccc
     4741 agaagaatac gattatcctt tagttagaaa agatatagaa gatagtggaa tacctacttt
     4801 atatgttgaa atcgaccaac aaactcagaa taatgaacaa gccagaactc gtattcaaac
     4861 ttttgctgag atgatgagtt tagcgtaagt tcattaaaat aaatttacta taaaatatga
     4921 aaattggagg aagctatgtt atataacaaa gaacaagaac ttttaagaaa agcagtaaga
     4981 gattttgtta gtaaagaatt agatacttta ccagcagaaa tggataaaac aggtgtaatg
     5041 cctaaggaat taatcaaaaa attagctgat gcaaaattta taagcagtaa tatcccagaa
     5101 gaatatggtg gtgggggagc aggatatgtt tcttacgcta tagtaatgga agaaatagct
     5161 agaagatgtg cttcaacagc tacttttgtt acagctggtt cttctcttgc ttcattacca
     5221 atactttaca atggtactga agagcaaaaa caaaaatact taaaaggtat agcaacaggt
     5281 gaattaatag gagcatttgg tttaactgag ccaggagctg gttctgatgc aggtggacaa
     5341 caaacaacag cagaattagt tggagaccac tatatattaa atggtagaaa aactttcata
     5401 actaatggac cattctgtga tgtagctata gtaatagcag ttacagatag aagtaaagga
     5461 cttagaggaa catcagcgtt tatagtagaa agtaaatggg atggtttctc aacaggagct
     5521 catgaagata agatgggtat aagaggaact gaaacttctg acttaatatt tgaaaacgtt
     5581 aaagttccaa aagaaaactt acttggaaaa gaaggtcaag gatttaagat agcaatgggt
     5641 actctagaag ttggtagaat aggtgttgct gccttagctc taggaatagc tcaaggtgct
     5701 ttagatgaag ctgttaaata tacaaaacaa agagttcaat ttggtaagcc tatagctaaa
     5761 ttccaaaata ctcaatttac tatagctgac atggaaacaa aagtttgtgc agctagagga
     5821 ttagtttatg atgcagcaca aaagagagat gcaggaatga gagttgctca agaatctgct
     5881 atggctaaat actatgcatc agaaattgca aatgaagttg cttataaagc attacaactt
     5941 cacggtggat atggatttat aaaagattat gaaatcgaaa gaatgtacag agatgctaga
     6001 atcgtatcaa tatacgaagg aacatcagaa gttcaaaaaa tggtaatttc atcaaacgta
     6061 ttaaaataat aaataatcta atgaaattgg aggtatagac tttgaaaata ttagtttgcg
     6121 taaaacaagt tccagatact aacgaagtaa gaataaataa agaaacaggt actcttataa
     6181 gagatggagt tccaagtata ttaaacccag atgatgcaaa tgcattagag gaagctttaa
     6241 aaattaaaga tgagcatgat gatgtaacta taacagttat aacaatggga cctcctcaag
     6301 cagactttat gctaagagag tgtttagcta tgggtgctga tgatgcaata ttattaagtg
     6361 atagagcatt tggaggagct gatacttggg ctacatcaaa tactattgca gcgggaatat
     6421 ctaaagtagg tgactatgac ataatatttg caggaagaca agcaatagat ggagatactg
     6481 cacaagttgg accacaaatt gcagagaaat tagacattcc tcaagttaca tatgtacaag
     6541 atttcaaaat agaaggaaaa gatataatag ttcaaagaca attagaagat ggatacgaat
     6601 taataaaagt aagtacacct gtacttttaa cagcagtaaa agagttaaat acacctagat
     6661 acatgtctgt tgataaaata gtaaaagcat ataaacatga tgtaaaagtt tggacaattg
     6721 atgatttaga tgtaaataaa gaagaagttg gtttaaaagc atcaccaact aaagtattta
     6781 gatcatttac tcctgaacca agaggaaaag gagaaatctt agaaggtaaa gctgatgaaa
     6841 tagctagtaa aataataatc ggtctaaaac aaaagcacat tatataagtt ggaggagtac
     6901 aaactatgaa tgatataaaa gatttaagtt cttataaaaa cgtatggata tttgcagaac
     6961 aaagagaagg aaaaatagct ccagtagtta tagaattatt aggagaagga agaaaattag
     7021 ctaaagaagt agatgcagaa ctttgtgcaa tattattagg aaaagatgtt gatggattag
     7081 ctaaagaatt aatcactttt ggagctgaca aagtttatgt tgcagatgat gctcttttag
     7141 aaaaatatac aactgatgca tatacaaaag taataaaaga tgcaatagat gaaataaaac
     7201 cagaaataat gcttttcgga gcaactcata taggtagaga cttagcacct agaatagctt
     7261 caagagttgg aactggatta acagctgact gtactaagtt agaaatagac ccagaagata
     7321 agaaaataaa acaaactcgt ccagcatttg gtggaaacat aatggctaca atcatttgtc
     7381 caaaccatag acctcaaatg tctacagtta gacctggtgt tatggataaa gctgaaaaag
     7441 acgaaacaag aactggtgaa gttatagcat tagactacaa aataactcaa gatgatataa
     7501 gaactactgt tttagaaaca gttaaaacta agaaagattt agtatctctt acagatgcaa
     7561 atgttatagt atcaggtggt ctaggattag gtggaccaga aggatttgaa atgcttaaga
     7621 aattagctga caaattaggt ggagtagttg gttcttctcg tgcggctgtt gatgctggat
     7681 ggatagacca ttctcaccaa gtaggtcaaa caggaactac tgttaaacca aacctatata
     7741 tagcttgtgg tatatcagga gcaatacaac atttagcagg tatgcaatca tcagatttca
     7801 taattgctat aaacaaaaac ccagcagctc caattttaga aatcgctgac tatggagtag
     7861 ttggagactt acatgaaata gttccaatgc ttatagaaaa attagatagc gttgatgatt
     7921 tattagaagc tataaaagct taatagatac aaactatata aatattactg attgaataag
     7981 tcttattagt tagtaacata attattaacc ctttgataag ttattgctag tgtatatttg
     8041 tcagttatta aaaagtctgt ttgaataaaa ttttataata caagataatc tggataatcc
     8101 ttatatgtat attgcttaca aattgtgagt tatattctgt aaggattatc tttgtttttt
     8161 taaataaatc ttaaatttaa ttaaattgta ttaaatagac aaataattac taaatatgtg
     8221 ataaaataca attaaaagca aaatgaaatt tttaacaaac tataatcatt aaaattatat
     8281 cttagttacg ttttaatgaa ataaactact tgaaaagacg aatattataa ggagttgatt
     8341 atatgtgctg atggtattta ttgtaagcaa acaataaatt ttgtatgtag ttcaataaat
     8401 tacttaatga aaggtgcgaa tattaatgga taataattta atagggctta tagaacatgt
     8461 agaaaatcca gccattttat gtaaagaatc aggcgaaata atatactgta atcatctaat
     8521 agatagtatt tttagttttc tagatataaa aaagcctaga aatataaatg aattagattc
     8581 aaattttgac aaaacagaaa tactgacaga tagtaaaaag aaaatagctt tcagagagtt
     8641 aaggatgact gcacatatat acaatatgaa agataataat aatgaaaata atatagttta
     8701 tttatttgag aagtctctaa tatctgataa agtaattgaa gatataatag agcatataga
     8761 tgaagttgtt gttgtgttta acaaagatgg ggtaattgaa aagatgaaca ctgtcagtga
     8821 tgagatatta ccttttaaaa gaactgaggt acttggtaga aatataacag accttgtgag
     8881 acaaggatta gttgaagaac caataatatt aaacatgctt aaagtgaaga aaaagattta
     8941 tagaaatatc gtttacccag atgggaagct tattgcatat acggcagttc caagatggga
     9001 ttctaaggga aagcttacag gaggagtact aacagggaga gatatttcaa gggttattaa
     9061 gcttgaatct cagataaaat atagtgatat atcagaagac acagagtata taagtcaaag
     9121 taagattatg gacaatataa aaaaagttgt taagagagct gcagcctcag attcttctat
     9181 atttataaat ggagaatcag gtgtaggaaa agaaataata gcaagaacaa tatataagta
     9241 tagttcaagg agagacaaac cttttatagc tataaactgt ggagctatac caaatgaatt
     9301 attagagtca gagttttttg gatatgaaga aggttctttt actggagcta agaaaaaagg
     9361 taaaaaggga ctttttgaag aggcgaatgg tggaactatt ttcttggatg agataggtga
     9421 gttaccaatg cagatgcaaa aaaaattact tagggttata caagaaaata ctattactag
     9481 aataggtggt agtaagccta taaaaataga tgttagatat ataagtgcta ctaatatttc
     9541 tcatgaagac cttagaaata accttaaatt tagacaagat ttgtactata gattaagtgt
     9601 tattccagta aaaatacctc ctcttagaga aagaaaagag gatatagtac cattagtaaa
     9661 ttatttctta aaactttaca atgaaaagta caatagagaa gtagaagttt cgcctaaagt
     9721 tattgagtta ttagaagaat attcttggcc aggaaacata agagaattaa aaaacatcat
     9781 tgaaagattt gtagtattat cagctaaaaa tgtaattgga gaagatgaat ttaacatgct
     9841 gataaattta gatatgattg ataatgaaac agatgattta tcaccaatag tggtaaatgg
     9901 aattatgaat cttaatgatg catataaaat agtggaccaa ataatgataa gtaaggctat
     9961 aaataagtat ggttcaataa caaaggctgc tgaagttata ggtatatatc cttctaccat
    10021 ccatagaaag ataaagagtg gacatattca cgtttagctg tcagtttaaa aagacaatat
    10081 aattaaaata ttataagata cataatttat acaacttaaa agagatggct taaaaataaa
    10141 tttaattact ttaagtcatc tcttttcttt tataaatatt taaaagttat attagtttat
    10201 agagttagca gaacctaaaa catcatctat cttgctttca actacagctt gtatagcatc
    10261 tctaccagca ccaatgtatt ttcttgggtc aaattctttt ggattttcag ctaagaattt
    10321 tctgatagca gcagtcatag ctagtcttaa gtcagtatcc atgtttattt tacaaacagc
    10381 catagaagat gcttttctta acatatctac aggtacacct ttagcaccag ctatatttcc
    10441 accaaattca ttacatgtag caacagcgtt ttggtcaaca gcagatgcac catgaagaac
    10501 tattgggaaa cctggtaatt tgctttggat ttcttctagt atatcgaatc ttaatttagc
    10561 ttctcctttg aatttgaaag caccatgaga agttccaata gctattgcta atgaatcaac
    10621 acctgttctt tcaacgaact caactgcctc agctggttga gtatacttgt gaacatcaga
    10681 agtaacatca tcttctgttc cagctagaac accaagttct gcttcaacaa caacaccttt
    10741 agagtgtgca tattcaacag cttctttagt tattctaaca ttttcttcaa agtcaaaatg
    10801 agaaccatct atcataactg aagagaaacc agcatcaata caagttttaa tagcatccat
    10861 atttggacca tggtctaagt gaagagctac atctacacct atttcatcag aagcagcctt
    10921 aaccatttca actaatgtat gaggtccagc atatttaaca gctgacatag aagcttgtat
    10981 cataacataa gaatttttag ctttagcagc ttttaaaaca ccttgtaatt gttctaagtc
    11041 acttatgtta aatgcaccta tagcaaatcc accttcgtaa gcttttttaa acatttcttt
    11101 tgtagtaatt aatgccattt taaactcctc ctattaactt ttaaaattta cccatcactt
    11161 atattataac gttaaatcaa aaaaagtata tagtaaattg taataactat aaattacaag
    11221 tataatgtta aatagaagac aataggatta aataaaataa taaagaatat aaacaattta
    11281 ggaaaactag ctatataatg ctaactataa gggtaggaac taccttatta gtttggagat
    11341 actgataaca atagttgcct tgaccaagaa gttaccactt caaacgttat cgaaaggaat
    11401 taagtggatg ataatttact aaaaatttaa aataaaggag ataaactatg ccacagataa
    11461 aaataagagg aattaatgaa aatgatatat gtaaaataag tgaaaaaatg ataaatgatt
    11521 tagttgaagc tgtaaaatgt ccaagagact actttgaaat agaatgtata aagtcagttg
    11581 caataagaga tggtaaaata gcagatgtat atccttttgt tgaagtagct tggtttgata
    11641 gaggacaaga agtacaagat atagtagcaa gaataataac tgatagtata agaaataatt
    11701 tagatgtaga aagtatagat ttagcattta cagtatttga aaaagaaaaa tattatgaga
    11761 atggagagca tttttaatgg ctaaaaagca acgtatagat aaaattcttt caaatttagg
    11821 ttatggaagt agaagtgaaa taaagaagta ttgtaaacaa gggtctgtag tagtaaatgg
    11881 aagtgaagtt tcaaatccag gaactcaagt agacactgaa aatgatgaga tattatttaa
    11941 tggagaagaa gttatatata gagaatatat atatttgatg atgaataaac ctgatggata
    12001 tatatcagca actacagata aatatgaccc aacagtatta gatttaattg atttatcata
    12061 cttagctttt gaaccatttc ctgttggaag gttagataaa gacacagagg gattattagt
    12121 tttaactaat gatggaaagc tttctcatag agttttatct cctaagaagc atgtgcctaa
    12181 gacttattat gcaaaaattg atggagtagt tactgaagaa gatgtagaag cttttttaga
    12241 aggtgtagtg cttgatgacg gatataaaac tatgccatca cagttaaata tattaaaaag
    12301 tgatgatgaa tcagaaatag agcttacaat acatgaaggt aagtttcacc aagtaaaaag
    12361 aatgtttgaa agcgtaggca aaaaagtagt atacttaaaa agattgtcta tgggcaattt
    12421 aaaattggat gaaagtttag agttaggtga gtatagagag ttaacagatg aagaagtaaa
    12481 attgattgaa gaaagataat aaagatacta tagtaaattg gaggaatgtc tgtgaagaaa
    12541 aaagatgtta tagaatttga agtagataaa atggaatttg gtggtacatc tttaagccaa
    12601 gttggagata gagttatcca tatgaagggt ggaataagtg gtcaaaaggt gagagctgga
    12661 gttaaaaaag ttagaagtaa gaaagctgaa gttaagatga tagaattatt agagaactca
    12721 ccacttgaaa ctgaagaaac ttgcaagcac tttagagagt gtggaggatg tacactattg
    12781 tctgtgccat atgaaaaaca attagaaata aaagaaaaac aagttatgga tttattttta
    12841 aagcaagatt tatttggatt tcagttttta gggatagaag gaagtccaga aaacaaatat
    12901 tatagaaata agatggaata tacttttgga gatgaagtaa aaaatggacc tttgacatta
    12961 ggtcttcata aaaaaggaaa gcatatagat atacaaactg ttgaggagtg tatgttggtt
    13021 gatgaagact tctcaaagat attagtttct agtgtagagt ttttcaatga aaaaaagctt
    13081 ccttattata gaacaatgaa tcacaaagga tatttgagac atcttgtagt tagaaaaggg
    13141 attcatacta atgagattat ggttaatata gtaacttctt ctcaagaaga ttttgatatg
    13201 tatgagttta aagatatgct tttagggatt gatttaaaag ctgagttagt aggtgtactt
    13261 catactatta atgatggttt agcagatgca gtccaatgtg atgagttaag agtattgtat
    13321 ggaagagatt atatacaaga agaaatttta ggtcttaagt ttaagatttc accattttcg
    13381 tttttccaaa ccaatactaa gggagcagag gttctttata gtattgctag ggattttatt
    13441 ggagattaca atgataaagt tgtatttgac ttgtatagtg gtactgggtc tattggacag
    13501 gttatggctg gtgctgctaa gaaagtgtat ggtattgaga tagttgagga agctgttgtt
    13561 gctgctaatg agaatgctaa gttgaatgga cttactaatt gtgagtttat tgctggggat
    13621 gtggctaagg ttgttaagga tttaaaggac aagcctgatt tgattattgt tgaccctcct
    13681 agaccaggca ttcataagga tgctattagg gatatttgtg gatttggtgc taaggagatt
    13741 gtttatattt cttgtaatcc taagtctttg gttgttgatt tggttgattt taaggggtat
    13801 ggttatgaga ttaagatggt taagtgtatg gatatgttcc caaatacgcc acattgtgag
    13861 actgtagtgt tgctacgtcg aaaataatgc tagaaatctt gagctttaga cagtttcata
    13921 gacatataat atttagatta gagggtaata aaattaggaa taaagaattg aaaaggtgtt
    13981 ttgatgtgtc tgtagttata tatatggagt gtgggaatac gaaataaaaa aagttttaat
    14041 tata
//
LOCUS       DAGSRM010000102        14022 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.102, whole genome shotgun sequence.
ACCESSION   DAGSRM010000102 DAGSRM010000000
VERSION     DAGSRM010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14022
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.102"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            15..866
                     /locus_tag="MJE33_003086"
     CDS             15..866
                     /locus_tag="MJE33_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HBY9649005.1"
                     /translation="MKNIISAKELISKLEKNDNLVVIDCRFDLINRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNDTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGELEEKVNIPSCTGKEIRPNINNDLVVPME
                     YVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSDFENGSLK
                     DIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
     gene            complement(999..2612)
                     /gene="ggt"
                     /locus_tag="MJE33_003087"
     CDS             complement(999..2612)
                     /gene="ggt"
                     /locus_tag="MJE33_003087"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HBY9649006.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGAVTVPGIPAAWSELNKKYGKLSLMECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSKKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELELEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKDGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPKHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVEPEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
     gene            complement(2837..4774)
                     /locus_tag="MJE33_003088"
     CDS             complement(2837..4774)
                     /locus_tag="MJE33_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9649007.1"
                     /translation="MTLFRIAMQNVKNSFFNYLMYFISIVFSVFVFFSFKSIEYNEAL
                     SSLGEKTRMSINTSSIVIVVFVFLFIYYSNSFFFNRRRREVGTYSLLGMRKNQIGKIF
                     LYETFLMGIVAILIGIFLGLLFSKLMTMMLVKLMNEMIVVNMNLSIKALVQTLAVFLI
                     IFIVIGIRNTIVIRNKRIIELFNKYPEKYKFKKFIKLKCVLGVLLIAISYLMSISYFI
                     AENIMLSVFILVTIIPGTFLFFSSTMYMIIDAVKKRKSFYYKGQNLIAFSELGFKLKS
                     NSSVLAVIAILIATSVTMLGFTISLYYDIDRNISENYKYSYTINLGNSYVNNEIDKLL
                     YKYKKDNKIIFDKTIELIDKDINIEMYYKNGNSYIEDRTSVDIIRESDFKKLRQYQNN
                     NYEELTSKESVYYVSDSYKKIFFKDFEIEKINLHIKDANEYDINLFKVQKSILEPEIN
                     SQATFDLIVVKDDVFNQLKLHGNSRLIRLIDIKNEKKELDLTLKLKNIVSENMKFTYP
                     FNFTSSIESYNNLIKLSGLMLFIGIFLSVVFLLCTGSIILFKQLSNIYDDKERYIMLI
                     KLGANNKDIEKIISKQLKVIFLMPLVVGTVHNLFAMSIAQKFIPRSLLVPIIITLVIY
                     FIGYFIYYFLTLKYALNMIKE"
     gene            complement(4761..5528)
                     /locus_tag="MJE33_003089"
     CDS             complement(4761..5528)
                     /locus_tag="MJE33_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9649008.1"
                     /translation="MEEILSVENIKKEYGRKGSKHEALRGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTIDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRIKEISKELNIESILDKYPGEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESNREFYNKIVNTVSLIGGVNKNDFI"
     gene            5816..6490
                     /locus_tag="MJE33_003090"
     CDS             5816..6490
                     /locus_tag="MJE33_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY9649009.1"
                     /translation="MFKIMVVEDDVSLKNIIAKCLTKWGHDVHQIENLENIIEEFKNY
                     NPELVLLDINLPFYDGFHWCNEIRKISKVPIIFISSRNSNMDVIMGVNLGADDYIQKP
                     FSVDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIS"
     gene            6514..7518
                     /locus_tag="MJE33_003091"
     CDS             6514..7518
                     /locus_tag="MJE33_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY9649010.1"
                     /translation="MSFKDFLKDKLGFLVYNFTFLIFTVSVIIFSPVDVFLTDTILYI
                     LVVNVVFLFLYLFISYIKKNKFLDKIKNDTFKINMNDIELARCKNEEKIYLNIIKNHQ
                     YQCNNIINNNIEKFEENKEILNMWVHDVKIPIAIIKLILEQNENLIDEKISDDIDSEI
                     FRIENSVEKILYLSRLEDFHKDFLIQEVNIEKIIRNIIKKYSKYFISKKIILDLNNLN
                     FKVLSDEKWLYFIFEQLLSNSLKYSSLGDNISIYCKRTKNYLQLRVEDTGCGIKKEDL
                     NRIFNKGFTGNNGRNNSKSTGLGLYLVKELCDKLGHKIDVDSEYNQYTKFTITFKCDL
                     "
     gene            7630..8859
                     /locus_tag="MJE33_003092"
     CDS             7630..8859
                     /locus_tag="MJE33_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBY9649011.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRNSSFGSSGFKSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTELQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIIAEIDSLKSQLSQYKKQKI
                     SLSDIAENKDAIASINAQITALNSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVANSSFKIPASCVLKDNKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     GDTTPNKKVEEVTMDVN"
     gene            8867..9541
                     /locus_tag="MJE33_003093"
     CDS             8867..9541
                     /locus_tag="MJE33_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9649012.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKALKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLAN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            9544..10776
                     /locus_tag="MJE33_003094"
     CDS             9544..10776
                     /locus_tag="MJE33_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9649013.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLVPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVIAKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFAATNYVSKYIGFEAIPSLNSLFYAIVATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(11062..12327)
                     /locus_tag="MJE33_003095"
     CDS             complement(11062..12327)
                     /locus_tag="MJE33_003095"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBY9649014.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMEAMIKAKELDVPLSFHEEDPSLVGNPGVNAGKVASELGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSKEEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSINPAKLYNLNIGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKVNYTICNGEIVYNA"
     gene            complement(12380..13528)
                     /locus_tag="MJE33_003096"
     CDS             complement(12380..13528)
                     /locus_tag="MJE33_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBY9649015.1"
                     /translation="MKSVSVRSCKTYNYEEVKLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQSSLKSIYKASGI
                     EEVANELGIKLNYDVSDVKIHSEKAHVLKYMDIIKPIVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVLLASQNPYAIDVVACKIISLLPNRVATIRGAIKRGYIQEDFSDIEILG
                     EDIKDLIIKNYKIPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPASTIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            complement(13628..>14022)
                     /locus_tag="MJE33_003097"
     CDS             complement(13628..>14022)
                     /locus_tag="MJE33_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBY9649016.1"
                     /translation="GVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYK
                     RIAFEEAEHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKRAKELGLDAI
                     HDTVHEMCKDEARHGKAFLGLLNRHFGK"
BASE COUNT         4893 a         2161 c         1785 g         5183 t
ORIGIN      
        1 aggaggtaca ttctttgaaa aacattattt cagcaaaaga acttatatca aaactagaaa
       61 aaaatgataa tctagttgta atagattgta gatttgatct tatcaataga acttatggta
      121 ttgattctta taaaaaagga cacatcaaag gcagttttat cttagatata gacaaggatt
      181 tatcatctcc tacaaaagaa catggtggta aaaatccttt acaagaccct ttaatactaa
      241 aagaaaaatt agaaaaaatg ggtgttgata acgacactac gattgtaact tatgatgatg
      301 gagatttaaa tggagcttgc agattatttt tccaattaaa acatttgggg cttaaaaatg
      361 tctatgtact agatggggga ataacatctt tcgtaaagga aggtggagaa ttagaagaga
      421 aagtgaatat accaagttgt acaggtaagg aaattagacc aaatataaac aatgatttag
      481 ttgttccaat ggaatatgtt aagtctaaat tatacaaaaa agatactgta ataatagatt
      541 gtcgtgctaa tgagagatac cagggtttag ttgaacctgc ttactcaaaa gctggtcata
      601 taccaagtgc aaaaaattat ttttgtaagg atttaataaa aagtgacttt gaaaatggct
      661 cacttaaaga tatagaattt ttaaaaaaat tctttaaaga tttaaataat tatgatgaag
      721 taattttatc ttgtggttca gggatatctg cttgtgttaa cagtcttgct cttagagaac
      781 tggatatacc tcacaagatt tatattggta gctttagcga ctggattagt tatgatgaca
      841 atgaaatcaa aactggtcaa gaataaggct cataatctat ataaaatcag catatgatag
      901 cttaaatttt gtaaaggcgt cagtcattgt agactttcgc ctttacttat tatttcatat
      961 actctttaaa tttattatat atattttagt tgataatatt aataaactgc aatatgacca
     1021 tcacatcttg attccgtccc acaaatataa ctttcctgtt ctgttttaaa tattatttga
     1081 cctcgtccca tgtcgacagt atcatgaatt acttttattt catgaccctt ttcaattaat
     1141 gaatctatta tatgctttgg catctctggt tcaacttcta tttcttttcc ttttatccac
     1201 tgccatcttg gagcatctag cgcctcttgt ggatttaatc caaaatcaat catatttgtt
     1261 aacacctgaa tatgtccttg tggttgcata aaagctccca ttacaccaaa tggacctatt
     1321 gctttatctt ctttacctaa aaatccaggt attatagtat gataaggttt tttaaatggt
     1381 tttactacat tgtggtgttt agggtctaaa ttaaaattat ttcctctact atgtaaagca
     1441 atcccagttc ttggtatcac aattcctgaa ccaaaattta tgtaattgct ctgtatatat
     1501 gaaaccatat ttccatcctt atctgcagta caaaggtata ctgtaccacc acaatatggc
     1561 tctcctgcag ttggatacaa agcctcttta ttgtcaatta ataaacttct ttttttggca
     1621 tactcttggc ttagtaattc ctgaatcttt accttcatat gctctatatc agttacatat
     1681 gttttagaat ctgcaaaagc taattttaaa ctctctataa ttttatgtat gtcttctaca
     1741 ttttctatat ttccttctaa ttccaattct tttaatatgt taagtgccat taaaactgtt
     1801 attccatgac catttggtgg gatttcaaat attttatatc ccttatattc tgtacttata
     1861 ggttctaccc atgttggata aaaacactct aagtcactct ttcttatagc cccattaaat
     1921 tttttagaat attcatctat tttatcagca agtcttcctc tataaaagct ttcagcctga
     1981 gtatttgcta tctcttctaa tgtacttgcc tgttcttcac atataaatat atctccagcc
     2041 tctggggctt taccatcttt agaaaatgta tcaaaccatg gtttgaactc ttctccaata
     2101 agttcttttt catagagttc atatgatttt ttccatacct tagctacatt aggagatact
     2161 acgtatccct ctcttgcata gtttatcgct ggagataagc attccattaa gctaagttta
     2221 ccatattttt tatttaattc tgaccaagct gctggtattc caggtacagt tactgctcca
     2281 aacccatatt ttggcatttc tttcatatta ttataatttt ttaattccat attttctgga
     2341 gcaaagccac tagaatttaa accatacaat cgtttttctt cctctatcca aacaagggca
     2401 tatgcatctc ctccaatccc attgcttgta ggttctacta ctgctaatgt ggcggctgtt
     2461 gcaactgctg catcaacagc atttccaccc ttttttagta tttcaagtcc agcttgagat
     2521 gctaatggtg ttgatgttgc aactgctcca tttttagcat acacgacatt tcttattgat
     2581 gagtatttat gttggtaggc attaaacttc ataaatttca gctcctcgtc attttttatt
     2641 tgatattctg cttagattta ttatataaca tcaagtaatg taactgatac aaaatgatat
     2701 aaaatataga ctttttccta actttaattt tatttttaaa taaatatctt aaactcaatt
     2761 ctagtatttt ctatctgcac ttaaataagt tattttattc aaaacatata tctaattctt
     2821 ttaacttaaa aataatttat tcttttatca tgtttagagc atattttaaa gttaaaaaat
     2881 aatatataaa atatcctata aaataaatga ctagagttat gataattggt acaagtaatg
     2941 accttggtat aaacttttgt gctatagaca tagcaaataa attatgaact gtacctacaa
     3001 ctaatggcat taaaaatata acttttaact gttttgatat tattttctca atatctttat
     3061 tatttgctcc aagttttatt aacataatat atctttcctt atcatcatat atatttgata
     3121 attgtttaaa taagattata ctacctgtac ataaaagaaa tactacagac aaaaatatgc
     3181 ctataaacag cattagacca cttaatttta tcaagttatt ataactttca atacttgatg
     3241 taaaattaaa aggataagta aatttcatat tttcacttac tatatttttt aattttaaag
     3301 tcaaatccag ttcttttttt tcatttttta tatcaattaa tcttattaat ctagaatttc
     3361 catgtaattt taactgatta aatacgtcat cttttactac aattaaatca aacgtagctt
     3421 gtgaatttat ttctggctcc aagatgcttt tttgtacttt aaacagattt atatcatact
     3481 catttgcatc tttaatatgc aagtttattt tttcaatctc aaagtcttta aaaaatattt
     3541 ttttgtatga atcagaaaca tagtacacag attcctttga agttaattct tcataattgt
     3601 tattctgata ttgtcttaat tttttaaaat cactttctct aataatatct acagacgttc
     3661 tatcttctat ataactattt ccatttttat aatacatttc aatatttata tctttgtcta
     3721 taagttctat tgttttatca aagattatct tattatcttt tttatattta tataataatt
     3781 tgtcaatttc gttatttaca taagaatttc ctaaatttat ggtataactg tatttataat
     3841 tttcacttat gttcctatct atatcgtaat aaagtgatat ggtaaaccct agcatagtta
     3901 cacttgtagc tattaatatt gctattacag ccaatacact actattgctc tttaatttaa
     3961 atccaagttc agaaaatgca attaaattct gtcccttata ataaaaactt tttctcttct
     4021 taacagcatc aataatcata tacatagttg atgaaaaaaa taaaaaagtt ccaggtatta
     4081 ttgtaactaa tataaaaact gaaagcataa tattttcagc aataaaataa ctaattgaca
     4141 ttaaataaga aatagctatt aataaaacac caagtacaca ttttaacttt ataaattttt
     4201 taaacttata cttttctgga tatttattaa ataactctat aattctctta tttctgataa
     4261 ctatagtatt tcttattcca ataactataa atattattaa aaatacagct aatgtctgaa
     4321 ctaaggcctt tatacttaag ttcatattta ctactatcat ttcattcatt aattttacaa
     4381 gcatcatagt cataagcttt gaaaataaca gtcctaaaaa tatacctatt agtattgcaa
     4441 ctatacccat caaaaaagtt tcatataaaa atatttttcc tatctgattt ttcctcattc
     4501 caagtaaact ataagttcct acttctcgcc tacgtctatt gaaaaaaaat gagtttgaat
     4561 aatatataaa taaaaataca aataccacta ttacaattga acttgtatta atactcatcc
     4621 tggttttctc tccaagtgaa ctcaacgctt cattatactc tatagattta aaactaaaaa
     4681 atacaaatac actaaataca attgaaataa agtacattaa ataattaaaa aaactattct
     4741 ttacattttg cattgcaatt ctaaataaag tcatttttat taactcctcc aattaaggaa
     4801 actgtattta ctattttatt atagaactct ctgttacttt ctccctttac aatttctgtg
     4861 aatattattc catcctttat aaagagtatt ctattgcaaa aactcgcact tatagcatca
     4921 tgagttacca tcaatattgt tttattgctg tctttgttta attttaatag acaattcaac
     4981 aaatccctag cagatttaga atctaatgct cctgtaggct catctgcaag tactaatgat
     5041 ggttttgttg ctatagctct acatacagct cctctttgct tttgtccacc agaaacttcg
     5101 ccagggtatt tatctaatat agattctata tttaattctt ttgaaatctc ttttattcta
     5161 ttttctatta ctgatactct ttcatttttt aaagaaagag gcaacattat attttctttt
     5221 attgtaagtg tatccaaaag attagaatct tgaaatacaa agcctagatt atcacgtcta
     5281 aaatctgcca attcattttg cttcatttta atagtattct ttccatttat ataaatatct
     5341 cctgatgaag gtaaatctat tgtagaaata atatttaaaa gtgttgattt accagcaccg
     5401 gaagacccca taataccaac aaattctcct ttataaacct tgaaagttat tcctcttaga
     5461 gcttcatgtt ttgaaccttt tcttccatac tcctttttta tattttcaac agataatatt
     5521 tcttccatag ttaataatca cctttcatca caattagtta tttatcaatt ctcacaaata
     5581 tattatatat attaaaatgg cttttaaaca tttaattata ttaatttaag gttacatttt
     5641 cgtaagtatt aattatattt cttcaaattt attgcaatta tgtttaaaat ataatttatc
     5701 aagtaaaata tagttatatt aatataatta ttttaggtaa taaggtagaa atattttaat
     5761 ttatcaagta gaatatagtt atgttaatat aactattttg aattaggagg taagaatgtt
     5821 taaaataatg gtagttgaag atgatgtctc attgaaaaat atcatagcaa agtgtcttac
     5881 taaatgggga catgatgttc atcaaataga aaatcttgaa aatataatag aagagttcaa
     5941 aaactataac ccagaattag ttttattgga cataaacctt ccattttatg atggttttca
     6001 ttggtgtaat gaaataagaa aaatttcaaa agttcctata atttttattt cttcaagaaa
     6061 ttctaatatg gatgtaataa tgggtgtaaa tttaggtgca gatgattata tacaaaaacc
     6121 tttttctgta gatgtcttag tagctaaagt taatgccctt ttaagaagaa cttataattt
     6181 tgttgataat aactcaaatc agattattca caatggtgtt actttagatt tatcaactgc
     6241 aacaataaac tatgaagata atactattga gctaacaaaa aatgaaatca aaattctcca
     6301 tgagcttatg aaatataagg ggcagatagt aagtaggaat aaactcatga aaaaattatg
     6361 ggataatgat tggtttgtag atgataatac attaactgtc aatgtaaata gaatacgctc
     6421 aaaattaaat gaaattggtt tagaagattt tatagaaaca aaaagaggat tgggttatat
     6481 aatcagttaa aacaattcta agtaggtgaa tttatgagct ttaaagattt tctcaaggat
     6541 aaactaggtt ttttagtata caattttact ttcttaattt ttacagtatc agttattata
     6601 ttttctcctg tagatgtttt tctgactgat actattttat atatattagt tgtaaatgtt
     6661 gtgtttttat ttttatatct atttattagc tacatcaaga agaacaaatt tttagataag
     6721 ataaaaaatg atacattcaa aattaatatg aatgatatag aattagctag atgtaaaaat
     6781 gaagaaaaaa tatacttgaa cattattaaa aatcatcaat atcaatgtaa taacataata
     6841 aataataata tcgagaaatt tgaagaaaac aaagagattt taaatatgtg ggtacatgat
     6901 gtaaagatac ctatagctat aataaaactc attcttgaac aaaatgaaaa tttaattgat
     6961 gaaaaaattt cagatgacat agatagtgaa atatttagaa ttgaaaactc cgttgaaaaa
     7021 attttatatt tatcaagact cgaagatttt cataaggatt ttttaattca agaggtaaat
     7081 attgaaaaaa tcattagaaa tattataaaa aaatactcaa aatattttat ttcaaaaaaa
     7141 attattttag acttaaataa tttgaatttt aaggttttat ctgatgaaaa atggctatac
     7201 ttcatattcg agcaattatt gagtaattcc ctaaaatact cctcattagg agataatata
     7261 tctatctatt gcaaaagaac caaaaattat ttacaattaa gagtggaaga tactggttgt
     7321 ggtataaaaa aggaagattt aaatcgaata ttcaataagg gatttacagg taataatgga
     7381 agaaacaatt ctaaatctac aggtcttggg ctttatttag taaaagaact ttgtgataag
     7441 cttggtcata aaattgatgt agattcagag tacaaccaat atacaaaatt tactattaca
     7501 ttcaaatgtg atttatagta tcttattatt gatagtttat tttcattaac ttatattcaa
     7561 cttcttatag taattctata tgtacatccc agtatataat taagtagttt ttataaaaag
     7621 gaggaagaca tgtcttttct aaaaaaattt aaaaataaaa gatttagaaa taacagtttt
     7681 aaaagtaaca actttagaaa tagcagtttt ggaagtagtg gcttcaagtc taaaaataaa
     7741 ggttttaaaa acaatccctt taaaaataag agattcaaaa tatatgcagg aatagcagta
     7801 atcatacttt taattctagg agttcttgct tatgttgatt cgaaaaacac tgaattacaa
     7861 gctaatgaaa attttatcga aacatatact attcctgaaa atgaaaaaat atttataaat
     7921 gggatggttg ttcctaagca aactaaggac ttcaatatat ctggagatta tgagttaagt
     7981 gatgttaatg ttacaaatgg tcaaaaagta aatcaaggag accttctttt tactgctaaa
     8041 aatccaacta taatagcaga aatagatagt ttaaaatcac aacttagtca atacaaaaaa
     8101 caaaaaatat cactttctga tattgctgaa aataaagatg ctattgcttc tataaatgca
     8161 cagataactg ctttaaactc tcagatagct tctctggata aaaaagctta tgacagagtt
     8221 actgctcctt ttgatggcac agtatacttg aatgaccaaa ctggaaatcc agaccaacca
     8281 gtttcattta tgactattca aggtctagag ttttatatga aaggacaagc aagtgaacaa
     8341 gatttaccta aaatgaagat tgaccaaatg gtcaatatac tagttttttc aactgaccaa
     8401 aagcttactg gtagaataag ttttatatct gataaaccat ctactccaaa tactgaaatg
     8461 ggagctcagc agaatacact atcttattat gatataaaca tagcatttga taatcaagaa
     8521 ggattagtaa atggattcca tcttcaagca tcacttgaag tagctaattc aagctttaaa
     8581 ataccagcat cttgtgtact taaagacaac aaacactcat atgtatttaa ggatttagat
     8641 ggtatattga aaaagcaaat tgtagatgta gcaagtcaaa atgatgattt tgctgtagtt
     8701 agaggcggtc ttgaacaagg cgatataata ataaaacacc ctactaaaga aatgaaagaa
     8761 ggagacccag ttcaaggcga tggcgtaact gctggttcta ataatggaga tactactcca
     8821 aacaaaaaag tagaagaagt tactatggat gtgaattaat ttacatatgt taattaaatt
     8881 agaaaatatt caaaaatatt acaaagtagg gaaagatgaa ttacatgttt taaaatcctt
     8941 aaatttagaa attgaatctg gagaatttgt catgataatg ggtaagtctg gtagtggtaa
     9001 aactacttta cttaatatct taggttttct agatgtattt gatgaaggaa gatatatatt
     9061 tgatggaaca gatgtaacta atttgagtga aaatgaacgt tctgttttta gaaatattaa
     9121 tattggattt gttttccaac aatttaattt aatagaaact ctaaatgtat atcaaaatgt
     9181 agagttgcct ctaatttaca ataaagcact aaaaaaatct aatagagaag aaattgttaa
     9241 agataaatta agttctgttg ggcttttgga caagcttaag cagaaaccac ttcaattatc
     9301 tggtggtcaa caacagcgtg tagccattgc acgttgccta gcaaatgacc ctcagataat
     9361 ctttgctgat gaaccgactg gggcattaga tagtgaaact agcagagaaa ttatggagct
     9421 tttaacaagg ctcaacaaac aaggtaagac tataataatg gttacacatg atcaagattt
     9481 aactaagtat gcaactaagg ttatacgact taaagatggt gtgtttactt cggaggtgta
     9541 atcatgagtt tattaaaaaa ttctttggcc aatttaaaag gtcacaagct tcgtgtattt
     9601 gtagctttac tctggattat aataggaata acttcagtaa tacttgttag ttctataggt
     9661 aatggatttc aaaaagaaat taagaaatct gtaaacaatg taaaccctaa taaaactact
     9721 atttcgtttg aatcagccga taacacaggt cttactgatg atatgagtat ttttttaaaa
     9781 ccctttaatg caaaagattt agaagaatta tcatttgttg aaggagtaga aagaatagct
     9841 ccttcaagag atgggtttaa ccttgattca gtttactctt cacaagcatc ttttgataaa
     9901 aaaactacgt atgttgatgt tggaccagta aaaaaagatt ctaaaataaa tttaatttgt
     9961 ggtagagatt tttctttaga tgatgaaaaa agaaaagtaa ttttacttac attacaaagt
    10021 acaagtgaaa tttttgaaaa tccagaagat gccttaggtc atggtatcaa cataaatgga
    10081 actatatttg aaataatagg ggtattagat gattctcagc aaaaccaagc tggaggattc
    10141 tttggaggtt accaagatat gcaatttact acttccctag tacctaaaaa ggcttttgac
    10201 actttaatga gtcaaaattc ttattcaaat gaaatttacc agttagactt agtttcttct
    10261 aaaggctata atgtaaatga agttgcaaac aatgttatag ctaaattata tgagatgcac
    10321 cctggtatca atggttctta tactacacca gacccaactg aacagacagc ttatcttgaa
    10381 agcattaatt ccaatgtaaa caaatatgta tctataataa ctgtagttgc aatgtttgtt
    10441 ggtggtattg gtgtaatgaa tataatgtat gtatctgtta tggaaagaca aagagaaatt
    10501 ggtataagac gtgctattgg agcaaaacct aggtctatat tatttcaatt tttagttgaa
    10561 gctgttttta taacagtatg tggtggaatt ttaggaacta tagttggatt tgctgctact
    10621 aactatgttt ctaaatatat aggttttgaa gctataccta gcttaaatag cttattttat
    10681 gcaattgtag ctactatatt gactggagtt gtatttggtc tgattccagc ctttaaagcc
    10741 tctaaactag accctataaa ggcaatttat aagtagaaat tgtataaaga ttttcgattt
    10801 ttagattagt ttatataaaa catacttaaa tatataacgc actattatat atgatagatt
    10861 aggaacttaa tctttccaaa tataatagtg cgttctttca taaaaaggat tctcttcctt
    10921 actatatcta aattaccaag atgttatgtt ctaattgttc aaactaatac ttccaatttt
    10981 gattaatcaa tctcattctc ctaatataca atcttttact ttcaatcatt gtacactccg
    11041 ccttaaaaaa ataatcttaa attatgcgtt atatacaatc tccccattac atattgtata
    11101 attaaccttt ccatataaag atttaccttt aaatggtgaa ttatcagctt tagaaacaaa
    11161 gctttcaact gtccattctt cttctggatt gaatataaca atgtctgcta cagccccttc
    11221 ttctataaat ccaatattta aattgtacaa ctttgcagga tttatagaca ttttttctat
    11281 taactgcatc atactaagat gatttttatg caccaaattt gttattccaa gagctaaaga
    11341 agtttcaagc ccaataattc cacttggagc ttttataaac tctctatctt tttcttcttt
    11401 actatgaggt gcatggtccg ttgctattat ttcaatagta ccatcattta atccctctat
    11461 aatcttatct ctgtcccatt tacttcttag aggtggattc atttttgcat ttgttccaaa
    11521 ctccaataca tcttcctcag tcaatgtaaa gtgatgtggt gaagcttctg caactacatt
    11581 tgcacctaag tattttgcaa atctaaccat atcaacagat acacctgaac ttatatgttg
    11641 tatatcaact ttagcaccag tctttagtgc caacatacaa tctctggcta ccataacatc
    11701 ttctgctaca cttgatgcac cttttaatcc tagttctgaa gctacctttc ctgcatttac
    11761 acctggattt ccaactaaag aagggtcttc ttcatgaaaa ctaagtggta catccaactc
    11821 ttttgcctta atcattgcct ccataattag attgctatcc attaaaggta atccatcatc
    11881 agtaaaccct ggtacaccag cttttttaag agcttccata tcaactaact ctttaccttc
    11941 aaaaccttta gttatagctg ctgcttgtaa aacatttata catgcatttt ttgacttttc
    12001 tttaacataa ttaaaagtat cttcattatc tacaatagga ttagtatttg ccatacatat
    12061 aacagttgta aatccacctc tagcagcaga tttagctcca ctttctatat cttctttata
    12121 tgtaaaccca gggtctctaa agtgtacatg tacatccact aaacctggag caactacatt
    12181 accactagca tctattattt tttcatattc atcactttca tcaaacttgc ctattttata
    12241 aattttattt tcttttataa ttaagtcaat attctcatct ctttgacttt tagggtctat
    12301 tactctacca tttttaatta aaatcatatt atttctcctt tatattttta atttatatag
    12361 actcatataa tattatacat tattttacat acttaaatac aaaattacgt ttgacatcaa
    12421 tagccttttt aggacaaagt tcatggcaac agtaacatct tatacattta cttaagtcta
    12481 taatagcacc cttagtccct atctctatag tggatgcagg acaagcttcc ttgcattttc
    12541 cacattttat gcaatcttga aatcttacca caggctttgg tgttataagc tttgaaatag
    12601 gttcatttaa aaattttggt agctttacag aaaacaatct taaatcctta cttacatttg
    12661 gaatcttgta attttttata attaaatctt ttatatcttc acctaaaatt tcaatatctg
    12721 aaaaatcttc ttgtatatat cctctcttaa tagcacctct aattgtagca actctatttg
    12781 gaagtaaact tataatctta catgcaacaa catctatagc atatggattt tgtgatgcta
    12841 ataaaactcc tatttctcga gtagtaccag ctgaaggtcc atcaccttcc atacctacta
    12901 taccatccat aattgtcaat gttggtttta tataatcaca aatatctaaa agtacatttt
    12961 cgcaaaacaa ctcttctgta ggaaatctat aatgtatctc tgcttttttc aatccagcta
    13021 tgcatccata taagtttttt gtaccaccag taaaagttgc cattatatga gtctttaatt
    13081 tacaaagatt tattatataa tctgaatcta ctattggttt aataatatcc atatatttta
    13141 gaacatgtgc tttttcacta tgtatcttaa catctgaaac atcataattt aacttaatgc
    13201 ccagttcatt tgctacttct tcaattccag atgccttata aatgctctta agagaacttt
    13261 gattatatgg accaccaggg ctatctgcta ttgtaatatc acaatttaac tctaaaagtt
    13321 tttcacatac aacttttaca accataggat gtgtagttgt agcttcttca ggcttttttt
    13381 tcattaataa atttggtttt ataagtactt tgcttccttt tttaacaaac ttatctatac
    13441 caccaatatc ctctaaattt ttttcaatag ataactttac ttcttcataa ttgtaagttt
    13501 tacaactcct aacagatacc gacttcatat tatctccatc cttctatcac aacagtttat
    13561 ttgtataatt taaaaaagga gaaaccaaag tttctccttt taatctatgt tttaattgtt
    13621 ttaattatta ttttccaaaa tgtctgttta ataatcctaa gaatgcttta ccatgtctag
    13681 cttcgtcttt acacatttca tgtactgtat catgtatagc atctaatcct aattctttag
    13741 ctctcttagc taattttaat tttccatcag ttgcaccata ctcagcatca actctaactc
    13801 ttaagttttc ttttgtatct gcaactacaa cttctccaag aagttctgca aatttagcag
    13861 catgttcagc ctcttcaaaa gctattctct tatacgcttc agctacttct ggataacctt
    13921 ctctatcagc ttgtctactc attgctaaat acattcctac ttctgtacac tcaccagtaa
    13981 agttagctct taatccttcg attatttctg catctacacc tt
//
LOCUS       DAGSRM010000103        13933 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.103, whole genome shotgun sequence.
ACCESSION   DAGSRM010000103 DAGSRM010000000
VERSION     DAGSRM010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13933
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.103"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..1155)
                     /locus_tag="MJE33_003098"
     CDS             complement(<1..1155)
                     /locus_tag="MJE33_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine/succinylornithine family
                     transaminase"
                     /protein_id="HBY9649017.1"
                     /translation="MNNDNTISKWNEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNSNLKNALKEQVDKLLHTSNLYFNEPVLCSGEKIINSSGMAKVYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYIERNNIEDLKNNLDSTVCAIILEAIQGEGGVNVLEKDYVLEIVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLVNKKLSKVLGKG
                     DQGTTFGGNLLAMVAASVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVVLKTKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYKEIDEALE"
     gene            complement(1185..2045)
                     /gene="argB"
                     /locus_tag="MJE33_003099"
     CDS             complement(1185..2045)
                     /gene="argB"
                     /locus_tag="MJE33_003099"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HBY9649018.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGIDNNMILCDPYKNYELGFVGEIKKVNVELIESCLTSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFEEGIITGGMIPKIEGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKENE"
     gene            complement(2060..3277)
                     /gene="argJ"
                     /locus_tag="MJE33_003100"
     CDS             complement(2060..3277)
                     /gene="argJ"
                     /locus_tag="MJE33_003100"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HBY9649019.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     NIKESDVLVASTGVIGKPLNIDLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEEHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            complement(3318..4352)
                     /locus_tag="MJE33_003101"
     CDS             complement(3318..4352)
                     /locus_tag="MJE33_003101"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HBY9649020.1"
                     /translation="MNKIKVGIIGATGYVGAELIRLLMNHDKVEVTAIGSNSYVGKDI
                     VDIYPSIGYKNNMICIENEKVIDMCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKEDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKNNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCKEKVSVVFTPNLIPVNRGILSTIYCTKEDNISINDIHRKLTDYYEFKEFIEIL
                     PLDKVASLKNVRFSNKCVISLHENGDTLIICSAIDNMIKGSAGQAIQNMNIIFGIEEN
                     TGLKNIAPSF"
     misc_binding    complement(4606..4819)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(5122..6213)
                     /gene="ytvI"
                     /locus_tag="MJE33_003102"
     CDS             complement(5122..6213)
                     /gene="ytvI"
                     /locus_tag="MJE33_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBY9649021.1"
                     /translation="MNEQDHTSYQNKQRFLINFCYLVVILGLLMLLGKYAFSVLLPFV
                     FAFLIASLLNKPVLYISEKLLIKRGIVATISVFLFFLIAGIIVSVIGTYLIYGIKEIF
                     HFLPTMFEELVIPLTETVIKEVELFSGSFNFSFIELIEANMPVLMNEMSVFIVSLSNN
                     IVSGITDIVSSVPLLFMKTIITIVASIFIAIDYPTIKRFIVLQIPKNKSYLLAEAKAF
                     TIDTIIKCGFSYLLIFAITFSELYVGFVIINISYAGIIALFIALLDILPVLGTGSILI
                     PWCIISLFLGDYSMALGIALLYIVITIIRNIIEPKLVGKHMELHPVLALASMLTGLHF
                     FGFIGLFGIPLLIAFLKKLNDKEIIHILH"
     gene            complement(6228..7619)
                     /locus_tag="MJE33_003103"
     CDS             complement(6228..7619)
                     /locus_tag="MJE33_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HBY9649022.1"
                     /translation="MKRRLAGEWEPIKGVMLAWPPFLPHKYIVDLTQNYSVYMCVPNK
                     EEIEIAKYYLSKWGGNLKNLIFCIAPQGFDTPWVRDWGMHPIFDENGKLFLAGAEYKM
                     STPFVSINKPEIMFDCEHNLLTTNSYEQKEDQSQELIAKQIGLPFMKLPYALTGGNLM
                     SDGFNKLMSMHILQTENRAKGISDEEFFSMVARDTGMNEYYIFSNFANYGLQHIDCYL
                     KIIDEETLLVSRTPKEHYLYERYEKILEEVKKVESRYGRPFKIFRIDIVPFQKDSDEL
                     TAYVNSVILNSRVYVPMYGIEQDKIAIKQWQNALPGYEIKGYEFDFEKEPDIIKNRTG
                     YVRTGWGNEDVIHCRTRAIWDENMLYISVKRLEEIVSENDALEVTIQIVDYSKAGLDY
                     ENCRLFYRYYGFQTWESIRLEETTEAEIFFANMIGKSGDMIEYFVQAKSCSGMCETMP
                     PVAPKGTYKVTIK"
     gene            complement(7638..8177)
                     /locus_tag="MJE33_003104"
     CDS             complement(7638..8177)
                     /locus_tag="MJE33_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY9649023.1"
                     /translation="MSLTIQKVTSYFKDLEKFEELNNEAFPDEERMSIQEMLNLVQSK
                     KIEVSALYDKQQFIGFYVLMIENHIGYILFLAISPEQRSKGYGGKALSLMKKQYPNCQ
                     IVLDMETIEDSAPNLQQRILRKQFYLQNGFYETEYLMEYKGLEMEVLCNQKTFQKNEF
                     ETLLKNLKIREIPFHLWKK"
     gene            complement(8198..9523)
                     /locus_tag="MJE33_003105"
     CDS             complement(8198..9523)
                     /locus_tag="MJE33_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009295908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBY9649024.1"
                     /translation="MIQLSDNFNYKRLLKFTFPTIIMLVISSIYGVVDGYFVSNFVGK
                     TAFTAVNFIMPFLLILGCVGFLFGTGGGALIAKTMGEGKKEEANEQFSLLIVTSAGCG
                     IILAVLGIVTMPWIASAMGADGQLLTDSILYGCVVIIAIPAYILQCEFQCLFATAEKP
                     KLGLYVAIMAGITNIVLDALFITVFKWGLVGAALATAIGQYVGGIIPLLYFSRQNNSL
                     LKFKKPKFDCDVLKKTVANGSSELMTNVSTSVVSMLYNAQLLKYAGEDGVASYGVLLY
                     VALLFQAVFIGYSVGTAPIISYHYGAKNYDELKNLRKRSLIVIICFAVFMFIIGEFLS
                     KPIAFIFVGYDNNLMEMTLRAFMIYSFSFLFSGFGVFGSSFFTALNDGLTSALIAFMR
                     ILVFQVVSVLIFPIFWGVNGIWFSIVGAEILATIMTAFFIIRKQKKYHY"
     gene            complement(9520..10143)
                     /locus_tag="MJE33_003106"
     CDS             complement(9520..10143)
                     /locus_tag="MJE33_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HBY9649025.1"
                     /translation="MKIITISREFGSGGRELGKQLADILNFDYYDKEIITAIARKKKI
                     DENYVSYILENHGWRNVPLTMCTSFTNILSMQSMKTDLLIEQSNVINQIASTGKDCVI
                     VGRNADILLKKYQPFNVFVCANMNFKIQRCIEKKLNNEHISKKELERKIREIDKNRAR
                     SREFFTSSLWGNQTSYHIIVNTSEWNIKHLSYAIANFSEYWFRRYEQ"
     gene            10235..11044
                     /locus_tag="MJE33_003107"
     CDS             10235..11044
                     /locus_tag="MJE33_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBY9649026.1"
                     /translation="MIKKLYKTSEFAELCGVKKHTLYHYDEIELLKPSITHENGYRYY
                     TIDQFEKFNIISVLKKAGTPLEEIKQYLNDLDTNTFLNVLHRKLMDLQEEVKKIEKMC
                     SMLRNVISTMEIHLNVNTEKIEIVNCEEEYLIATKIPEAVLTDEKTMLQGIGNHLQYC
                     LLHDYNFHVGEIVLEKNIKQGIFRESYYYSLIKNKVDDERLFIKPKGNYAVMYHQGSY
                     DSLYKTYQNMKNQLEEQGYHIVGNIYEEDLIDYLSEHNAENYIQKISMQVI"
     gene            11478..11840
                     /locus_tag="MJE33_003108"
     CDS             11478..11840
                     /locus_tag="MJE33_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY9649027.1"
                     /translation="MEIDYKAIGQRIKIARIKKGITQESVANIINMTPSHMSNVETGK
                     TKVSLPTLIAIANALSVSVDTLLCDNVLSSKVIFEKEAKDIFSDCDEYEIRSLVELLK
                     SAKTVIRKDREIRKQFEK"
     gene            complement(12190..12747)
                     /locus_tag="MJE33_003109"
     CDS             complement(12190..12747)
                     /locus_tag="MJE33_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY9649028.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAEKLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNSQMIKAAIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSLL
                     SKEEAIKGLVDSIDTVLLGIHKKYD"
     gene            13005..13703
                     /locus_tag="MJE33_003110"
     CDS             13005..13703
                     /locus_tag="MJE33_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBY9649029.1"
                     /translation="MEKKYFEIHGVPSILWGNPSSKLYIYIHGQCGNKEEAEAFANIA
                     VPHECQVLSVDLPEHGERKREKNSFNPWHVVPELVSIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLRECIFVSPILDMQQLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPITQWNVPTKILYGKHDELTEIGVVEKFVEKFNCNLTIIENGKHWFNTPQ
                     ELNSLYEWLNTLVK"
BASE COUNT         4788 a         2456 c         1593 g         5096 t
ORIGIN      
        1 ttcaagagcc tcatctattt ccttataact gattgttaat ggtggtaaaa atctcaatac
       61 attttttcca gcagtcaaaa taagcaatcc tttttttcta gctttctctt ctataatact
      121 agattcaata ttagtttcaa ttcctatcat tagaccaata ccttttgtct ttaatacaac
      181 tttgttatta aaactttcaa tagattttct tatataattt cctttttcta atacttcatt
      241 ataaaaacca tctttagata tctcatctaa tacaactgaa gctgccacca tagctaataa
      301 attacctcca aaagttgtac cttggtctcc ttttcctagt acttttgaaa gttttttatt
      361 taccaatact cctcctactg gtattcctgc tcccaaccct tttgctacgg taactatatc
      421 tggttttaca tcaaaatatt catagccaaa aagtttacca gttcttccta taccgcattg
      481 gacttcatca aaaatcacta caatatcctt ttcttgacat atttttacta tttccaaaac
      541 ataatctttt tcaagaacat taactccacc ttcgccttga atcgcttcaa gaattattgc
      601 acaaactgtg gaatcaagat tattttttaa atcttcaata ttatttcttt ctatatattt
      661 aaaaccttcg ggaagaggat aaaaatactt atggtatttg tccataccag ttgccattaa
      721 agacatcatt gttctaccat gaaaagagtc ttttaaactt atgatagttc ctctaccatt
      781 accatatttg tcactactat actttcttgc tattttgaat gctgtttcat ttgcttctgt
      841 tccagaatta caaaaatata ctttagccat tccacttgaa ttaattattt tctctcctga
      901 acacaacact ggctcattaa aatataaatt tgaagtatgt aataatttat ctacttgctc
      961 ttttagagca ttctttaaat tactattact atacccaaga gaagatactc catatccact
     1021 tgtaaaatct atgtatttat tgccattagt atcaaaaaaa caacttcctt caccataatc
     1081 tatgacaaaa ttgggttggt tatatgtatc tataaaatat tcattccatt ttgaaattgt
     1141 attgtcgtta ttcattctta ttctcccctc ttaaacttat aattttattc attttctttt
     1201 cttattaatg tacctattcc actatcagta aatagctctg ttattataga atgtggaact
     1261 cttccatcca aaatatgtac cctatttact ccgttattta aggcatctac gcatccctct
     1321 attttaggta tcatacctcc agtaattata ccttcttcaa ataatttatc aacatcctct
     1381 aatataactt ctgttattaa tgatttttct tcatctggct ctcttaacaa accagggaca
     1441 tctgtaagaa gtatcaattt atctgcattt aactctttag ctatagcaga agctgctgtg
     1501 tcaccattta tattataagt ctctccatca tctccaacac ctatagttgc aattactgaa
     1561 atataacctg acgttagaca agattctata agctcaacat ttactttttt gatttctcct
     1621 acaaatccaa gttcataatt tttatatggg tcacataaaa tcatattatt atctattcca
     1681 caaagcccta ctgcttttcc accttttgta tgtattttat ttactaggtc tttattaacc
     1741 tttccagcaa gtaccatttt tacaatttcc atggtctctt catctgtata tctaagacca
     1801 tttacaaatt tactttctat atccactttt gctagcatct tattgatttc tgcccctcca
     1861 ccatgtacta aaactatatt gattccaaca taactcatta acacaaggtc ttccataaca
     1921 gattctttta gaccatcttt tttcattgca cttccaccat acttaactac aatagtcttt
     1981 ccctgatgtt tttctatata tggtaaagct tctataagtg tatttgcttt ttctatatta
     2041 atcattaaag tatatcctcc taatttctat aacttccatt tattcttaca taatcatagc
     2101 ttaaatcaca tccccatgca tttcctctgg cattgcccat atttaaatct actaatatac
     2161 ttatttcgtc atcttcaagg attttttttg ctttttcctc attaaaatct aatccatttc
     2221 catttttaca tacttcaata tatcctttca tactctcaaa aataatatca actttttcca
     2281 tatctatttc ttcacctgca taacctaaag cacacagtat tctaccccaa tttgcgtcag
     2341 ctccaaatac agctgtcttt actaatgaag aagatataac tgtttttgct aattttactg
     2401 catgttcttc acttaatgct ccatttatat agcattcaat tagttttgta catccttctc
     2461 catcttttgc aacaagttta gcaagttcta tacagacata tgtaagtgca ctcaaaaata
     2521 cttgataatg ttcatctttt ttgtttattc tatcattttt tgcttttcca tttgctagaa
     2581 tcattaccat atcattagta cttgtatctc catctacact aactctatta aaacttttgt
     2641 caacagcaat ttttaaagct tcttttagca aaactccatc aatatttgca tctgtagtta
     2701 taaatcctaa cgttgttgcc atattaggat gtatcatgcc agaacctttt gccatacctc
     2761 caatagttat ttttttatct ccatacatta ttgctactgc tatttctttt tttataatat
     2821 cagttgtcat aatagcttct cttgctccaa tatgaccttg tttacttaga ttattcacta
     2881 attcatccat attttcttct attaaatcta tatttaatgg ctttcctata acacctgtag
     2941 atgcaaccaa aacatcactt tctttaatat ttaatttatc actggtgaat ttagccattt
     3001 tataagcatt tataagtcct tcttttccat taaaggtatt agcattagca ctatttacta
     3061 tgatagcttg tgcctttcca tttttaatgt gttcctgtgt aacataaaca ggattgccct
     3121 ttactttatt ttgagttact actgctgctg catcacataa aacatctgaa taaaccaaag
     3181 ctaaatctct tttttcttta ttttttctca agccacagtg aatccctgaa gcaaaaaatc
     3241 cttctgaaac tgtaactcca ccctttataa tctccatatt tatcctccta attatatgag
     3301 tttagtattt tttatttcta aaatgatggt gcaatatttt ttaaacctgt attttcttca
     3361 ataccaaata ttatattcat gttttgtata gcttgacctg ctgatccctt aatcatatta
     3421 tctatagctg aacagattat caatgtatct ccattttcat gtaatgatat tacacattta
     3481 tttgagaatc taacattctt taaagaagct accttatcta atggaagaat ttctataaac
     3541 tctttaaatt cataataatc agttaatttc ctatgtatat catttataga aatattatct
     3601 tcttttgtac aatatatagt tgataagatt cccctattta ctgggattaa gtttggtgta
     3661 aaaactacac ttactttttc tttgcaggat tcacttaaat tttgttctat ttctggagta
     3721 tgtctgtgtt tccctatttt atatgcagtg atattttcat taacctccgt aaagtgacta
     3781 ctaatcgata attctctacc agcacctgta agacctgatt tagaatcaat tattatatta
     3841 ttatttttta tcaatttaga acttagtaat ggcatcaatg gtaaagaaat actagttgga
     3901 taacatccag gatttgcaat tatatctgca tcttttatat cttctttgta tatttcactt
     3961 aatccatata cagctttttt atgtaagatt ttatctataa aagatactcc ataccattta
     4021 ctatatactt cttcatcttt tattctaaag tcagcaccta aatctataac tttctttttc
     4081 gatttaattg cctttattac aaacttttca ctaactccat gtggaagggc tgtaaacaca
     4141 acatcacaca tatctataac tttttcattt tctatacata tcatgttatt tttatatcct
     4201 atacttggat atatatcaac tatatctttt ccaacatacg aatttgaacc tatcgctgtc
     4261 acttcgactt tatcatgatt cattaataat ctaatcaact ctgccccgac atatccagtt
     4321 gcaccaataa taccaacttt tattttattc attttgttcc ctccattttt atattcaaat
     4381 agtcttaaaa tcctatcttt ccactttact atatgataat catttgataa tacgtagatt
     4441 aaaatactct aattaaaaca aaaaacccct tagcattata aatactaaga ggcgattttc
     4501 cgcggtacca ctcttattgg taaaattaca ataatcatta aaactcaaat taaaaagtat
     4561 ctacaaaatg tacatttaaa attaaaaaag cctcttggtc aaagaccaag agacgaatat
     4621 acacccgcgt taccactcta attgataaga taatcttacc cactctaatc tcttaaggcg
     4681 agattcacca attaccatac tattacttct ggtaatctcc tccaaagctc tattcacaat
     4741 aaaattcgtt actaggcttc caccattccc agctctctat caactagctt tatgctactc
     4801 tctttttcac aggatttcaa tttattttct tattgtttta tatgatagtt tattttttta
     4861 actttgtcaa tataattttt aagtttttgg aaatattttc tatataatta atttccagca
     4921 ttttgtatct aaattgagtt ataaatatct acagtaatag gacaccatga atttcctgct
     4981 gtaatatatt ttaaaaaagt tctccctctt gaatcaagtc attcaaaagc tatacctttg
     5041 tcaaaattat ttttcatcca gcgtttttta gaaaaaacac aattttctcc ataaaagaga
     5101 catacataca actattcttt cctaatgtaa tatatgtatg atttctttgt catttaactt
     5161 tttcaaaaac gcaattaaca atggaatacc gaacaaccca ataaatccaa agaaatgtaa
     5221 tcctgttagc atactggcta aggccaatac aggatgtaac tccatatgtt taccaactaa
     5281 ttttggttca ataatattcc tgataatagt aatcacaata tatagtagtg caattcctaa
     5341 tgccattgaa taatccccta aaaataaact gataatacac caaggtatta gaatacttcc
     5401 agttcctaaa acaggtagta tatctaaaag agcaatgaat aaagcaatta ttccagcata
     5461 agaaatatta ataataacaa aaccgacata taattcagaa aaagtgatag caaaaattaa
     5521 aagataagaa aaaccacatt taataatggt atcaatagta aacgcttttg cttctgctaa
     5581 aagataactt ttatttttag gtatctgtag cactataaat ctctttatcg ttggataatc
     5641 aattgctata aaaatagaag caacgattgt aataattgtt ttcataaata gtaatggaac
     5701 agacgaaaca atatcagtaa taccagatac gatattattt gaaagagaaa ctatgaaaac
     5761 actcatttca ttcatcaata caggcatatt tgcttctatt aattcaataa acgaaaaatt
     5821 gaatgaacca gaaaatagtt cgacttcttt tataactgtt tcagtcaaag gtattaccaa
     5881 ttcctcaaac atggttggta ggaaatgaaa aatttccttt attccatata ttagatatgt
     5941 acctattaca cttactatta ttcctgcaat taaaaagaat aaaaaaacag atatggttgc
     6001 tacaatacct cgctttatta gtaatttttc acttatgtat aagactggtt tattcaataa
     6061 acttgctatt aaaaatgcaa aaacaaaagg aagtaataca gaaaacgcat atttccctaa
     6121 taacattagt aagcctaata tcactaccaa ataacagaaa tttattaaaa atctctgctt
     6181 attttggtat gatgtatgat cttgttcatt caaataaaat gcacctccta ctttatagta
     6241 actttatatg tgccttttgg agcaacaggg ggcattgttt cacacattcc agaacaactt
     6301 tttgcttgca caaaatattc aatcatatca cctgactttc ctatcatatt ggcaaaaaat
     6361 atttctgctt ctgtagtttc ttctaaccta atactttccc atgtttgaaa accatagtat
     6421 cgataaaaca gtctacaatt ttcataatct aagccagctt tactataatc aactatttga
     6481 atagtaactt ctaaagcatc attctctgaa acgatttctt ctaatctttt cacggaaata
     6541 tagagcatat tctcatccca aattgctctt gtcctgcaat gaataacatc ttcatttccc
     6601 cagccagttc tcacatatcc tgtacgattt tttataatat ctggctcttt ttcaaaatca
     6661 aactcatatc ctttaatttc atatccaggc aaagcgttct gccattgttt tattgcaatt
     6721 ttatcctgtt caattccata cattggaaca tagacacgac tatttaaaat cacagaatta
     6781 acataagcag taagttcatc actgtccttt tgaaacggaa caatatctat acgaaagatt
     6841 ttgaatggac gtccatatct gctttctacc ttttttactt cttctaaaat tttttcatat
     6901 ctttcataca aatagtgttc ttttggcgtt ctgctcacaa gtaatgtttc ttcatcaatt
     6961 atttttaaat aacagtcaat atgttgtaaa ccataattag caaaattaga aaatatataa
     7021 tactcattca ttccagtatc tcttgccacc atagaaaaaa attcttcgtc agaaattcct
     7081 ttcgcacgat tttctgtctg taaaatgtgc atagacatca atttattgaa accatctgac
     7141 ataagatttc cgcctgttag tgcataaggt aatttcataa atggcaaacc aatctgtttc
     7201 gcaatcaact cttgtgattg atcttctttt tgttcataag agttagtagt taataggtta
     7261 tgttcgcaat caaacatgat ttctggttta tttatggata caaatggtgt tgacatctta
     7321 tattctgcac cagccaaaaa gagttttccg ttttcatcaa aaatagggtg cataccccaa
     7381 tcacgaaccc atggtgtatc aaatccctgt ggagcaatgc agaaaatcaa attttttaaa
     7441 tttccacccc acttacttaa ataatatttt gcaatttcaa tttcctcttt atttggtaca
     7501 cacatgtata cagaataatt ttgcgttaaa tctacaatat acttatgggg aagaaaaggt
     7561 ggccatgcaa gcatgacacc tttaatcggt tcccattccc cagctaaacg tcttttcatt
     7621 tctaatacct cactttctta ctttttccac aaatgaaatg gaatttctct aattttaaga
     7681 tttttcaaca atgtttcaaa ttcatttttt tgaaaggttt tctgattaca taaaacttcc
     7741 atttctagtc ccttatactc catgagatat tctgtttcat aaaacccatt ctgaagataa
     7801 aattgcttcc ttaaaattct ttgctgtaaa ttaggagcac tgtcctctat agtttccata
     7861 tcaagaacaa tttgacaatt gggatattgt tttttcataa gtgacagtgc ttttccacca
     7921 tatcctttag agcgttgttc tggggaaatt gcaagaaata atatatatcc tatatgattt
     7981 tcaatcatca aaacataaaa accaataaat tgttgcttat catataaagc agatacttct
     8041 atttttttac tttgaactaa attaagcatt tcttgtatag acatacgttc ttcatctgga
     8101 aatgcctcgt tattaagttc ttcaaatttt tctaaatctt taaaatatga agttactttt
     8161 tgtattgtta agctcatata gatacctcca aattgattta ataatgatat tttttctgtt
     8221 ttcttattat aaagaaagcc gtcataatag ttgccaatat ttcagcacca acaatagaaa
     8281 accatatccc atttactccc cagaatatag ggaatataag tactgataca acttgaaata
     8341 ctaaaatacg cataaaagca ataagcgcag aagttaaacc gtcatttaat gcagtaaaga
     8401 aactagaacc aaaaacacca aaaccagaaa ataaaaagga aaaagagtaa atcataaatg
     8461 ctcttaatgt catttccatt aaattattat cgtaacccac aaaaataaaa gcaatcggtt
     8521 ttgataaaaa ttcgcctatt ataaacataa atacagcaaa acaaataatt acaatcaaac
     8581 ttctttttct taaatttttt aattcgtcat aattttttgc tccataatga taactgatta
     8641 taggggcagt accaacagaa tacccaataa aaacagcttg aaatagtaac gctacataaa
     8701 gaagaacacc ataggatgca acgccatctt cgccagcata ttttagtaat tgagcattat
     8761 acagcatact cacaacagat gtggaaacat tagtcattag ttctgaagaa ccatttgcaa
     8821 cagtcttttt caaaacatca caatcaaatt taggtttctt aaatttaagc aaactgttgt
     8881 tttgtcggct aaaataaagt aatggaatga taccaccaac atattgaccg attgcagttg
     8941 ccagtgctgc acctaccaat ccccatttaa aaacagtgat aaataatgcg tctaaaacaa
     9001 tatttgtaat tccagccata attgcaacat ataatccaag ttttggcttt tctgctgttg
     9061 caaataaaca ctgaaattcg cactgcaaaa tataggcagg aatagcgata attaccacac
     9121 atccataaag aatactatct gtcaataatt gaccatctgc tcccatagca gaagcaatcc
     9181 atggcatagt tactatacct aaaacagcta gaataatacc acaaccagca gaagtaacaa
     9241 tgagtaacga aaactgttcg tttgcttctt ctttttttcc ttcacccatt gttttagcaa
     9301 ttaaagctcc accacctgtt ccaaaaagaa agccaacaca ccctaagatt agcaaaaatg
     9361 gcataataaa attgacagca gtaaaagcag ttttccctac aaaatttgaa acaaaatatc
     9421 catcaacaac cccataaata gaacttatta ccagcataat aattgtagga aatgtaaatt
     9481 ttaataagcg tttataatta aaattatctg acaactgtat cattgttcat acctcctaaa
     9541 ccaatactct gaaaagttag caattgcata agaaagatgt tttatattcc attcagatgt
     9601 attaactata atatgatagg aagtttgatt tccccataag gagctcgtaa aaaattctct
     9661 tgatcttgct cgatttttat caatctctct tatctttctt tctaattctt tttttgaaat
     9721 atgttcattg tttaactttt tttcaataca gcgttgtatt ttaaagttca tatttgcaca
     9781 aacaaaaacg ttaaatggct ggtacttctt taataatata tctgcatttc taccaacaat
     9841 aacacaatcc ttacctgtac tggcaatctg attgataaca tttgattgtt ctattaacaa
     9901 atctgtcttc atggattgca tagacaaaat attagtaaaa gaagtacaca ttgttaaagg
     9961 aacgtttctc caaccatgat tttctaatat gtaggaaaca tagttttcgt caattttttt
    10021 ctttctagct atagcagtga taatttcttt atcgtagtag tcaaaattta aaatatcagc
    10081 aagttgtttt ccaagttctc gtccaccact tccaaactct cggctaattg taataatttt
    10141 cataatatct tttcctcaca tttcattgaa tataaaagct ttctatgtta tcctaaagta
    10201 tctggtaacc ctaaagtcaa taggagattt aaaaatgatt aaaaaattat ataagacaag
    10261 tgaatttgca gaattgtgtg gggtaaaaaa acatacactt taccattatg atgaaattga
    10321 attattaaaa ccgagtatta cccatgaaaa tggctatcgg tattatacaa ttgaccaatt
    10381 tgaaaaattt aatattattt ctgtgctaaa aaaagcaggt acacctttag aagaaataaa
    10441 acaatatctc aatgatttag ataccaatac ctttcttaat gtacttcaca gaaagctaat
    10501 ggatcttcaa gaagaagtga agaagattga aaaaatgtgt tctatgttga gaaatgtcat
    10561 ttcaacaatg gaaatacatc tcaatgtaaa cacagaaaaa atcgaaattg taaattgtga
    10621 ggaagaatat ctgattgcaa ctaaaattcc tgaagctgta ctaacagatg aaaaaacaat
    10681 gttacaaggt attggaaatc acttacaata ttgtttatta catgattata attttcatgt
    10741 aggagaaatt gttcttgaaa aaaatataaa acagggaatt tttagagaga gctattatta
    10801 tagccttatt aaaaataaag tagatgatga acgccttttt attaaaccaa agggaaatta
    10861 tgcagttatg tatcaccagg ggagttatga cagtttatat aaaacttatc aaaatatgaa
    10921 aaatcagtta gaagaacaag ggtatcatat tgttggaaat atttatgaag aagatttaat
    10981 tgattattta tcagaacata acgccgaaaa ctacattcaa aaaatttcta tgcaggttat
    11041 ttgaaaatat aaaaagcgtg tatatctcaa atcacatgag aaatacacgc cttttttaat
    11101 atctcgtatt gtctataata atttgcttaa aatgtccttt tttatcttct aaagttggta
    11161 tgttatttcc aaatttttgt agaaattcag gttcattgct attatttgtt tcctcaatgc
    11221 aaactgcatt tcctcatgaa tttcagatac agaaatagct ttttattttc gttaaccatt
    11281 taatcagttt tttactatct ttttataaaa tcttaacatg gaggctttat gcctccattt
    11341 cttttgtagt tgtcttaaaa gtagtgcaaa tttttttctt aaatatatat tatataaatt
    11401 attcatttat atagagtatt atattatgct ctgtaaatag ttacaatatt taaaagaatg
    11461 aaaggagaac aaaaactatg gaaattgatt ataaagcaat cggacagcga attaaaattg
    11521 caagaattaa aaaaggtata acacaagaat ccgttgcaaa cattatcaac atgacaccat
    11581 cccatatgag caacgtggaa acaggaaaaa cgaaagtcag cctgccaacc ttgattgcga
    11641 ttgccaatgc actttctgta tcagtcgata ctctcttatg tgataatgta ctttcttcca
    11701 aagtaatttt tgaaaaggaa gccaaagata ttttctcaga ttgtgatgaa tatgaaatcc
    11761 gttctttagt tgaactgcta aaatcagcaa aaactgtaat ccgtaaagac agagaaatcc
    11821 gcaaacaatt tgaaaaataa aaccacagct tcaaggtata aattgatatg tgttgttagc
    11881 taaggtcttt tcttttttta tataatcatt tttatataat caaataacct atccaataat
    11941 tttccaatta ctgtccttat gaaatatcaa tttatactat gatacctgtg tcgcttttgt
    12001 ctgattatca aggaatttta cagctacctt ttcattatca acgacaacag taaaaatagg
    12061 gtttaccaat tcagaaagta aatattcttg aaatatacta ttttcctttc ataaaaatga
    12121 taaaatttta ttttacatcc cacgtaatga agtgttcaat aaataagtta aaataattta
    12181 tttacctcct taatcatatt ttttatgaat acctaataac acagtatcaa tagaatcgac
    12241 tagacctttt atagcttctt ctttactcaa aagtgatttc ccaagttgca aagtcgaata
    12301 accatgaagt acactcatta ttaagtttaa tacttcacct gtattctttg tatttagatt
    12361 acaagaaaga ataagttttt ctaataagga tttatattca tcaaagattt ctaatgtttc
    12421 attatttcca tgccaagtag cccactgaat tgtttcataa acaccaggat tagaaattat
    12481 aaaattaaag tatgctatac ttatacactt tatagctgta tctccaaaat ttccaatagc
    12541 cgcctttatc atttgactat tcatctctct cattccttta tgagctactt caagtaataa
    12601 atcatctaga ctttctatat gattgtacaa agatggtgta cgtattccta gcttttctgc
    12661 aataactttt aaagaaactt tgttaagccc atctttatta actatatctg atgtagcttg
    12721 tataactatt tctctactaa cacgcatgat tttccctctt tcatttaaaa ttaattactt
    12781 tattagttta actaatctaa ttagtttcgt caattattgt aatttataaa attctttata
    12841 ctattataaa atcttgacag tttaattata atttaatata atactaattg aattagtttt
    12901 aatactaata taattagtat tatattaaat tctatattac aacattattt tatagtttga
    12961 ttaatttaac aatcactaat atatcaaact gaaaggagat ttttatggaa aaaaaatatt
    13021 tcgaaattca tggagttcct tcaatccttt ggggaaatcc ttcaagtaaa ttatatattt
    13081 acatacatgg acaatgtggc aataaagagg aggctgaagc ttttgctaat attgcagttc
    13141 cacatgagtg tcaagtttta agtgttgatt tgcctgaaca tggagaacgt aaaagagaaa
    13201 aaaattcatt taacccatgg catgttgttc cagaattagt atctattatg gaatatgcaa
    13261 agtgccactg gaatcaaatt tcgttatatg caaatagcat tggagcatgg tttagtatgt
    13321 taagctttga aaaagagaac ttaagagagt gtatttttgt atctccaatt ctagatatgc
    13381 aacagctcat ttcaaatatg atgctttggg caaatgtatc agaagaacag cttaaacatg
    13441 aactaattat cccaacttca tttgggcata ctctttcatg ggaatatcta caatatgtaa
    13501 aacaacatcc tatcactcaa tggaatgtac caacaaaaat attatatggt aaacatgatg
    13561 aacttactga aatcggtgtt gtagagaaat ttgtagaaaa gtttaattgc aaccttacta
    13621 ttatagaaaa tggcaaacat tggtttaaca caccacaaga attaaattca ttatatgaat
    13681 ggttaaatac tttagttaaa taaaaaataa ttatattatt aatatgatta aattatttat
    13741 aaatgttatt taattaattt tattttagca ttattaaaat aactacataa agaataaatt
    13801 agttattatt aaagaaatta aaagtatata gagtattaat aattttttaa taattagtta
    13861 ttatagaaaa aagagggata taatattgtc tccctctctt tcttctgtaa tattatataa
    13921 tttttattta tca
//
LOCUS       DAGSRM010000104        13636 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.104, whole genome shotgun sequence.
ACCESSION   DAGSRM010000104 DAGSRM010000000
VERSION     DAGSRM010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13636
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.104"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            23..1153
                     /locus_tag="MJE33_003111"
     CDS             23..1153
                     /locus_tag="MJE33_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY9649030.1"
                     /translation="MNLKDFNGKRLKSARIFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNIENTLKLSNILNFPKEYFYKNENINIVVEDSHFNPQSKLPRVEEISYREKIIIIH
                     KIYSFMENYIKFPKENLPNRKVMSDININDIESLAYKTREFWDLNSTPIVNMVSLLES
                     KGIIISGMNVDRKGATIFTQKQRISKESKYLISLGNDKKSASIRNYTLACELGYIISS
                     ELRIPSKQFSEDEYACAFLLPKESFLKDLTHPEDLDYYVELKKKWIVPISAMIFRAYN
                     LEKINYKKYNYLMNEMDKKGWLIEEPLDKMKGSSPTYLKRAVELLIENKIMSVNSIVS
                     SLEEFRINLYPEDLELLMGLKKGLLSQEVNKKSKVIKFDGKK"
     gene            complement(1270..2238)
                     /locus_tag="MJE33_003112"
     CDS             complement(1270..2238)
                     /locus_tag="MJE33_003112"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBY9649031.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFVVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTRL
                     RRILSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILDMIKDNPSTYSNLDLP
                     SIVEKNKKTGISIINGKDCTEFGIGTALVEIVKAILHNEKKVLPVSTLLEGQYNERNV
                     FAGVPCVIGKDGIEEIIEINMTEYEQNEFNKSCSVLREYIALSKTL"
     gene            2572..2760
                     /locus_tag="MJE33_003113"
     CDS             2572..2760
                     /locus_tag="MJE33_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649032.1"
                     /translation="MNYHTKEELVEALRVVSSSIINCGKGQKKFSEETSHHTRFKNII
                     EVMYISKSLIMDEISKRD"
     gene            3020..4219
                     /locus_tag="MJE33_003114"
     CDS             3020..4219
                     /locus_tag="MJE33_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein AlaX-L"
                     /protein_id="HBY9649033.1"
                     /translation="MEKLYYTNQYIRDFTAEIIEIKEVENKYHVLLDKTAFFPGGGGQ
                     FGDLGKIENDDVINVYEDGKKIYHVLDKKPIKIHKVKCSIDWDRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGNEYSTVDIEGNLEEDKIREIELFANEIIRQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVIKIGDLDTNACCGVHPKSTGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDEVLKRDIYLTKICRYLSCSEEDAINGIVNLNSKVEEALIKKRRLE
                     EVVSTYQVKEMIEKANKVGNISVIKKIYDDEDLKYVNKIANKIAEYENSIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAISLIDGKGGGSKVLAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            4271..4774
                     /locus_tag="MJE33_003115"
     CDS             4271..4774
                     /locus_tag="MJE33_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY9649034.1"
                     /translation="MEQQKDYIELYENEELIEIREARMDDLDTIAKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENINAKATYESLNMYECDYNMY
                     EYEVVHS"
     gene            4881..5198
                     /locus_tag="MJE33_003116"
     CDS             4881..5198
                     /locus_tag="MJE33_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649035.1"
                     /translation="MGVLKGMFYVFLFLDLVSIFCFFFNKGKIASNKIVFNAIGVLTF
                     VLCFMLFSYYPNNNLIGKFIASLFFIFGVAGVMLKEKNFLYARLLLTVVIVFSTLRLF
                     VIQ"
     gene            5356..6315
                     /locus_tag="MJE33_003117"
     CDS             5356..6315
                     /locus_tag="MJE33_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBY9649036.1"
                     /translation="MIESKKSEKLLLSIIYIIFLVYILMIIRIILFRDVPIYAIFKGT
                     FRNVNLIPFYTIYQFIVDSNLDFMKATVNIIENIGIFIPMGIFLPIVCKNLNKKTIII
                     VIILVSLAFELTQYIFALGSSDIDDVILNSLGGIIGINIYINMNKLFPSDINRFKAII
                     GTSLVLGVIGLGVISKNYHNLLTFKFRPDRKISKILIEENREIIKDINKDTVDIVGTF
                     ESFKKGIITIKAGSNNKVKRPENLIDSDGNIRIYLNENTKLVSYIITKESKMDIVKYE
                     EFDVKNLDLLRRYDTINVWIDKENQPKDKHGIMASKLLIGLYE"
     gene            6430..7215
                     /locus_tag="MJE33_003118"
     CDS             6430..7215
                     /locus_tag="MJE33_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649037.1"
                     /translation="MEIIKYILYFFIYSFLGWTVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTILEYFTGFILETLFHAKWWDYSDRKFN
                     IKGRVCLKNAIYFGVMSVLIIRFIHPFIKYFVSIIPYRILISMAIITTLWTILDLIVT
                     IITLKKLDIKLNLLDDIITDLNDINVKLDKFDRGEIQALFKTINNDELEVREKINKIN
                     SKLDRIESNIILQKRVIKAFPHIKHKKQQEQLEHFKKIINEKR"
     gene            7507..8826
                     /locus_tag="MJE33_003119"
     CDS             7507..8826
                     /locus_tag="MJE33_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBY9649038.1"
                     /translation="MSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYEGN
                     EYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIAPG
                     DNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNMRR
                     KMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGIIVP
                     PVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGGLP
                     LGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYIKA
                     GFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIFLG
                     QSTLRVQPPLVITKEQVDKAMNIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(8901..9329)
                     /locus_tag="MJE33_003120"
     CDS             complement(8901..9329)
                     /locus_tag="MJE33_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649039.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYPKN
                     RKKDDKKKGNKKKNTNTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAILII
                     TKLTDSTNKGNYSRNTVSDTHKNDIRNIVFNESNNFLICG"
     gene            complement(9420..10796)
                     /gene="hydG"
                     /locus_tag="MJE33_003121"
     CDS             complement(9420..10796)
                     /gene="hydG"
                     /locus_tag="MJE33_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HBY9649040.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIKEEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(10797..11882)
                     /gene="hydE"
                     /locus_tag="MJE33_003122"
     CDS             complement(10797..11882)
                     /gene="hydE"
                     /locus_tag="MJE33_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HBY9649041.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLSLDEILE
                     CCDIGYKIGYRTFVLQGGEDAYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNEDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSGRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(11918..13126)
                     /gene="hydF"
                     /locus_tag="MJE33_003123"
     CDS             complement(11918..13126)
                     /gene="hydF"
                     /locus_tag="MJE33_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HBY9649042.1"
                     /translation="MSLNSTPQSVRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGELGELRIGKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLTKNKVKCPVVSVSSTDKIG
                     IENLKNEIIKVLPKDSTEFKLVSDLIEPNDLVVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDNGAISIVTKEDSLKETLSNLGKKPKLVITDSQVFPQVDKDTPKDIPLTSFSILFAR
                     QKGDLKELINGAYALENLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGVLIAYVKGI
                     LERSLELFNY"
     gene            complement(13141..13392)
                     /locus_tag="MJE33_003124"
     CDS             complement(13141..13392)
                     /locus_tag="MJE33_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HBY9649043.1"
                     /translation="MKKVAVISAILEEPKECQFKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDTINSLTGKLGNIPHVSVKTSISKKEV"
BASE COUNT         5110 a         1635 c         2091 g         4800 t
ORIGIN      
        1 aattttgaaa gagagaaaac aaatgaattt aaaagacttt aatggaaaga gattaaaatc
       61 agctagaatt tttagagcaa agacgataga acagttgtct aaagaaacta aaataaataa
      121 aaaagattta aaagcttttg aggaaaataa gtatgtacca aatatagaaa atacattaaa
      181 attatctaat atattaaatt ttccaaaaga gtatttttat aaaaatgaaa atataaatat
      241 cgttgtagag gatagccact ttaaccctca atctaaacta cctagagtag aagagatttc
      301 ttatagagaa aaaataatta taatacataa aatatattca tttatggaga attatattaa
      361 gtttccaaaa gaaaacttgc caaatagaaa agtaatgtca gatattaata taaatgatat
      421 agaatctctt gcatataaga ctagagagtt ttgggattta aatagtactc caatagtaaa
      481 tatggtaagt ttgttagagt ctaaagggat tataatatca ggtatgaatg tagatagaaa
      541 aggggcaact atctttactc aaaaacagag aattagtaaa gaatctaaat atctaatatc
      601 tctgggtaat gacaaaaagt cagcatcaat tagaaattat actttggcat gtgaacttgg
      661 atatataata tcaagtgaat taagaatacc aagtaagcaa ttttcagaag atgagtatgc
      721 ctgtgcattt ttattgccaa aagagagttt tttaaaagat ttgactcatc cagaagattt
      781 agattattat gtagaactaa agaaaaaatg gattgtacct atatctgcta tgatttttag
      841 agcatataat ctagaaaaga taaattacaa aaaatacaat tatcttatga atgaaatgga
      901 taaaaaaggc tggttgatag aagaaccact agacaaaatg aaaggttcaa gcccaacgta
      961 tttaaaaaga gcagttgaat tattgataga aaataaaata atgagtgtaa atagtatagt
     1021 atcaagttta gaggaatttc gaataaatct ttatccagaa gacttagaat tattgatggg
     1081 attgaaaaaa ggattgttat ctcaagaggt aaataaaaaa agtaaagtta taaaatttga
     1141 tggtaaaaaa taggtttata ttttagtatt aaaattaaaa gcaggtacaa atgatttaaa
     1201 cattaactta gttaaaagtt ttttcaataa gtcatttacc tgcttttagt gtaagaaaga
     1261 atattgaatc taaagtgttt ttgaaagtgc aatatattcc cttaaaacag aacaagattt
     1321 attaaattcg ttttgctcat attcagtcat attgatttca ataatttctt caataccatc
     1381 ttttcctata acacatggaa cacctgcaaa tacatttctt tcgttatatt gaccttctag
     1441 taatgtagaa actggaagaa cttttttctc attgtgtagt atagctttta caatttctac
     1501 taaagcagta cctataccaa attctgtaca atctttacca tttattatac ttattcctgt
     1561 ttttttattt ttttcaacta ttgatggtaa gtctaaattg ctataagtac ttgggttgtc
     1621 ttttatcatg tctaagattg gtttaccacc aattgaaaca tgagaccaag gaaccatttg
     1681 agaatctcca tgttcaccca tagaataagc ttgaattgat tgttgagcga ttccagtttc
     1741 ttcagataaa atccttctaa gtcttgttga atctaaagct gttccagttc caatgacttt
     1801 gtttttagga aagccagatt tttcccaaat ataatttgta actacatcaa caggattaga
     1861 tatgactaca aatataccat caaagccaga atcaactatg ggttttacta tagattttat
     1921 cattccaata gttggtctta gtaaatctaa tctagttgtg ttttgtctac gtaaaggccc
     1981 ttctgaagag tctgcaacac ttatcactac tatgtcagaa tctttacagt cagaaaaagt
     2041 tcctttttct atatgtactt tatgtggcag atatgaaact gcatcagcta aatctaaagc
     2101 ttgagctttt gatttagatt catctatatc aatcataaag agttcatcac aaactccttg
     2161 agttattaaa ctaaatccac aatgagaacc aacatgccct gaacctacaa tagatatttt
     2221 tcttggcttt attgccattt taaatcactc cccctaaaat aacgatacat tgaaaattat
     2281 agatgttatt aaaagctgtt tttggatatt ttaatattct attatttcag aaaatttatt
     2341 taatatgatt ttatactaaa atttttaaaa aagcaatgtt aatatactct ctattagtga
     2401 atattaaagt attatatgta tattaataat tattaaaata tgtaatgtca ctgatagtta
     2461 gaatatttaa gtgctaaaag atgtgtataa ataataatga tgtcagttta taatgatatt
     2521 aaattgagat atactgtttc tctaaattaa aaaataaagg agtaaatggt tatgaattat
     2581 catacaaaag aagaattggt ggaagcactg agagtagtat cttcaagtat tattaattgt
     2641 ggaaaaggac agaagaaatt ttctgaggag acatctcatc atacacgttt taagaatatt
     2701 atagaagtaa tgtatatttc aaaatcatta ataatggatg aaattagtaa aagagattga
     2761 agcttatttt gcaatttaac atgttttaat atacatttta ttatataaga aaataagtaa
     2821 acatacatgt tataaaatat tgaaataata tacaattata aatttgttaa cattaatatc
     2881 tatatgttaa aatatagtag aagatgttag taaataatag gatgagttct attttaatac
     2941 aattaaatta agcttagttt gaaatttgta tctaaaaagt aaagtaatct agaattaaga
     3001 aaatggagat agaattataa tggaaaaatt atattataca aatcagtata taagagattt
     3061 tacagcagaa ataattgaaa taaaagaagt agaaaataag tatcatgttt tattagataa
     3121 gacagctttt tttcctggtg gaggaggtca gtttggagac ctgggaaaaa tagagaatga
     3181 tgatgtaata aatgtgtatg aagatggaaa aaaaatatac catgtattag ataaaaaacc
     3241 tataaagata cataaggtaa agtgtagtat tgattgggat agaagagaag atggaatgca
     3301 tcaacatttt ggacaacatg tattgtctgg atgttttttt aaaactttta atgcaaatac
     3361 gacaggtttt catttgggaa atgagtatag tacagtagat atagaaggta atttagaaga
     3421 ggataaaatt agagagatag agttgtttgc caatgaaatt attcgtcaaa atatacaagt
     3481 agagacatta cttccaacga aaaaagaact aaaaaagatt tggttaagac gtgatttgcc
     3541 aaatactgat gaagagataa gagtaataaa aataggtgat ttagatacga atgcatgttg
     3601 tggagtgcat ccaaaatcaa ctggtgaatt gagaatgata aaaataaaga aatgggaaaa
     3661 acataagtca agtacaagaa tagagtttct agcaggtaaa agggcagtgg atgaagtatt
     3721 gaagagggat atatatttga ctaaaatatg tagatattta agctgtagtg aggaagatgc
     3781 tataaatggt attgtaaatc taaatagtaa agtggaagag gctttaatta aaaaaagaag
     3841 gcttgaagaa gtagtatcta catatcaggt caaagaaatg attgaaaaag caaataaggt
     3901 tggcaatatt tcggtcatta aaaaaattta tgatgatgaa gatttgaaat atgtaaataa
     3961 gatagcaaat aaaattgcag aatatgaaaa tagtattgca ttattagctg ttaaatttaa
     4021 tgataaaata aatttagttt ttgcatgttc taaaaattta aaagatatta atatgggagt
     4081 tttattaaag gatgcaataa gtcttataga tggcaagggt ggtggaagta aagtattagc
     4141 tcaaggagga ggtaagaata atggaaattt agaggctaca cttgattatg catttatgaa
     4201 gatagaaaaa actctataaa tatattatat aaaaaacctt agtagataaa atagatatgg
     4261 aggaataagt atggagcaac aaaaggacta catagagtta tatgaaaatg aagaactaat
     4321 agagataaga gaagcacgta tggatgattt ggatacaata gcaaaattta attataattt
     4381 ggctaaagaa actgaaggaa aagaacttga tatggatgtt ttaactaaag gtgtaaaagc
     4441 attgttatta gatgagagaa aaggaaaata tcatgtatat acagtatttg ataaagttgt
     4501 tgcacagatt atgtatacat atgagtggag tgattggaga aatggaaatt tcctttggat
     4561 tcagagtgta tatgtagata aggaatatag aagaaaaggt atctttaatt atttatttaa
     4621 ttatataaaa aatatatgtg ataaagatga aaatatagtt ggaatgagat tatatgtaga
     4681 aaaagaaaac ataaatgcaa aagcaacata tgagtcttta aatatgtatg agtgtgatta
     4741 taatatgtat gaatacgaag tagtacattc gtaatataaa tttgtaatac tgttatactt
     4801 atgttataaa gtattgatta tattgacgat tatatgaaat aaactggata ttataattag
     4861 aatttttagg gggtataaaa atgggcgttt taaaaggaat gttttatgta tttttatttt
     4921 tagatttagt gtcaatattc tgtttctttt tcaataaagg aaaaatagca tctaataaaa
     4981 ttgtgtttaa tgcaatagga gtacttacat ttgtactttg ttttatgctt tttagttatt
     5041 atcccaataa taatcttatt ggaaaattta ttgcttctct attttttata tttggagttg
     5101 caggagttat gttgaaagaa aaaaattttt tatatgctag acttctttta actgtagtta
     5161 tagttttttc tacattaaga ttgttcgtta ttcaataaaa tgtatttgta tgaaaattta
     5221 ttaaagtcta cacatttaag tgtaggcttt tttgattttt tgtttgaaaa ttaaggaaat
     5281 tttaaggaaa ttttaaaatc gtcttaaata tttattgata tacttaaaat attaatagcg
     5341 aaaggagact tatcagtgat agaaagtaaa aagtcagaaa aattgttact gtcaattata
     5401 tacataatat ttttggtata tattttaatg attataagaa taattttatt tagagatgta
     5461 ccaatttacg ctattttcaa gggaacgttt aggaatgtaa atttaattcc attttataca
     5521 atatatcaat ttatagtaga tagtaatctt gactttatga aagctactgt aaatattata
     5581 gaaaatatag gtatatttat tcctatggga atttttttac ctatagtatg taaaaatcta
     5641 aataaaaaaa ctataattat agtaattata cttgttagcc tagcatttga attaacacaa
     5701 tatatttttg ctcttggaag tagtgatata gatgatgtta ttttaaattc attaggtggc
     5761 ataattggaa ttaatattta cataaacatg aataaattat ttcccagtga tattaacaga
     5821 tttaaggcta taataggtac tagtttagtt ttaggagtta taggattagg agttatttct
     5881 aaaaattatc ataacttact aacttttaaa tttagacctg atagaaaaat atcaaaaata
     5941 ttaattgaag aaaataggga aataattaaa gatataaata aagatacagt agatatagtt
     6001 gggacatttg agagctttaa aaaaggaata ataactatta aagcaggttc taacaacaaa
     6061 gtaaagagac cagagaattt aattgattct gatggaaata taagaattta tttaaatgaa
     6121 aatacaaaat tagtatctta tataattacc aaagaatcaa aaatggatat agttaaatat
     6181 gaggaatttg atgtgaagaa tttagattta ttaaggagat atgatactat taatgtttgg
     6241 attgataaag aaaatcagcc aaaggataaa catggtatta tggctagtaa attattaatt
     6301 ggattgtatg aataaaatat taaacaagaa taaaagaata acaaatataa aaaacgaaaa
     6361 aactatttct ataattattt ttacgttgtg cataaaataa gaacattagt gaaggagtag
     6421 agataaatta tggagataat aaaatatatt ttgtactttt ttatatattc atttttagga
     6481 tggacagtag aaagtatagg atgttctata gcaagcaaaa gaataatcaa cagaggtttt
     6541 ttaaatggtc caatatgccc tgtttatggt tttggagcgg taattgtgat atcactattg
     6601 ggaagattta ataatgtagt aatagtattt ctattaggaa tgattttaac tacgatacta
     6661 gaatatttta caggatttat tttagaaacc ttatttcatg ctaaatggtg ggattattct
     6721 gacagaaaat tcaatataaa gggtagagta tgtttaaaaa atgcaatata ctttggtgta
     6781 atgtctgttt taataataag atttatacat ccatttataa aatattttgt aagtataatt
     6841 ccatatagaa tattaatttc aatggctata attacaacac tttggactat acttgatttg
     6901 attgttacta ttataacact taaaaaatta gatataaagt taaacttatt agacgatata
     6961 attacagacc ttaatgatat aaatgtgaaa ttggataaat ttgatagagg agaaatacaa
     7021 gctttattta aaacaataaa taacgacgaa ttagaagtta gagaaaaaat aaacaaaata
     7081 aatagtaagt tggaccgtat agagtctaat attattttac aaaaaagagt tataaaagct
     7141 tttccacata taaaacataa aaaacaacaa gagcaactag aacattttaa aaaaattatt
     7201 aatgagaaaa ggtaattata caatttaatg aagacgaata gaataatggt atttataaga
     7261 tgattttcag aggactctat aaaaatagaa tagagtcctt tattttttga aaaataatgt
     7321 ttatttgata aaaataaaat atattgtttc tgaataacaa atattttgaa aattataaaa
     7381 acatatattg acaatttgtg tcaaaaatac taacattaag tatataaaga attttatatg
     7441 agaatatctt tataaatcgc aataatttta aaacataaat ttatttaaag aggggatgat
     7501 ttgccaatga gcgagataac tagtcaaatg ataagtacag aagaaaaaaa atatgttgct
     7561 aaaactcaaa agatacctta ttatccagta gcatttaaat caggtgatgg tgcaatgcta
     7621 tatgattatg aaggtaatga atatgtagat tttttagcaa gtgcaggctc tgcaaatgta
     7681 ggtcatggta ataaggaaat ctcacaagca gttaaagaac aaatggatga tataacacaa
     7741 tatacacttg catatttcca tagtgatcct cctgtaaaat tagctgagaa gcttgtggaa
     7801 atagcccctg gagataatga taaaaaagta ctatacagtg caactggttc tgcttgtata
     7861 gatgctgcta taaaattagc aagagggtat actggaagaa caaagataat atctatgtgt
     7921 gaatcttatc atggaagtac ttatggagct atatctatat ctgctttaag tacaaatatg
     7981 agaagaaaaa tgggtccatt gttaccagaa gtttatcact tccattatcc tgataaaaat
     8041 agaacagcta aagaatgttt agatgagata gaatatgctt ttgcacacta tctaccagca
     8101 gaagaggtag ctgcaatatt tatagagcca attgcaggag atgcaggaat aatagtgcct
     8161 ccagtagagt gggtacaagg tttaagtaaa atatgtaaag aaaatggaat attacttgta
     8221 agtgatgaaa tacaacaagg tatgggaaga acaggtaagt ggtttggtat agaaaatttt
     8281 ggtgttgaag cagaccttat agttcttgga aaatctgttg gaggtggatt acctctagga
     8341 gcagttgttg gaagaacaga aataatgcaa agtcttgatg caccagcaca tttatttact
     8401 ttagctggaa acacaacagt atgtgtggct gctttaaaat ctatagagat aattgaaaaa
     8461 gaaaacttac ttcaaaagag tatagaaatg ggagattata ttaaagcagg atttgaaaaa
     8521 ctaaaagaaa aatacgatat aattggagaa ataagaggca taggattatc tataggagtt
     8581 gatatagtta aaggaaaagg ttctaatgaa aaacatccag atgctacagc taagatatgt
     8641 tatagatgta tacaaactgg attaataatg atattcttag gacaatctac tttaagagtg
     8701 caaccacctc tagtaataac taaggagcaa gtagataaag caatgaacat catagattct
     8761 gcaatagatg attatttaaa tggtagaatt ggtgatgaag tatatgaagt aactcaaggt
     8821 tggtaaaata aatattaaat gaaaatagga gttatctcaa ttttagaaaa ttatttctga
     8881 aatgagataa ctcttttaag ttacccacaa ataagaaaat tgtttgattc attaaagact
     8941 atatttcgga tgtcgttttt gtgagtatca gagacagtat tacgagaata attaccttta
     9001 ttcgtactat ctgttaattt agttataatt aagatagcta aaagaataag aattactgtg
     9061 tataaagcaa taacatatgg ttttctttta tatttgtatt tgttattgaa aaaagaatgg
     9121 ccataattat ttttatttac tttagatgta tttgtattct ttttcttgtt acctttcttt
     9181 ttatcatcct tttttctatt tttagggtaa ggaatgactt tgtgtgtatt atcaagtttg
     9241 atatttttat ttttttgaat attgttttcc aggctagcct ttttttctcc aaattgaata
     9301 gttttattgg tattgttgtt atctttcaat gttaatctcc ctcccttaaa ctttcccttt
     9361 agataatatt actactaacc tatggaatat acaataaata atatataagt aataaaattt
     9421 tataggtata aatctctttc tccacgctct aaggcagtca tattgttaga tactatgttt
     9481 cttatatctt ctctttctat cttacctatt tcttctgcga taagtttttt acccataatt
     9541 ttaagttctt catctccata atcgattaaa aattcattta atgtagtaag agcatttggt
     9601 gtacatacat tatggatttg tcgactttta gctaagctca tgaatctatc gccagttcta
     9661 cctttacgat aacaagctgt acaataacta ggtatatatc catcagtaat cagttcttta
     9721 agcacctcaa cagggcttct atggtctcca acttcgaatt gttctgtatt gtcaccatct
     9781 tcatatgctt tatatcctcc aacacctgtt agagaacctg cactaatttg tgatacacca
     9841 tattttagta actcatttct catttcagca gtttctctag tggacattat gattccagta
     9901 aaaggaacag ctaatctagt tatagctact atttttttga acatatcgtc agaaactaag
     9961 tgaggaaaat cttctaagct cataccttca gcttttttca atctagggaa agatatagta
    10021 tggaaaccta caccaaattt ttcctctaag tgttcgttgt gcatcataag tcctagaact
    10081 tcaaatttag ggtcagataa accaaataaa actcctgcac caacatcatc aacaccagct
    10141 tccatagctc tatcaaaagc tgttaaatgg taataatagt cattttttat agattgacca
    10201 tgcattttta taaatgttgg tttgtgataa gtttcttgga ataatatata agttcctatt
    10261 cctttttctt ttagtaattt atactcatct acacttgtag ctgctatgtt aacgtttacc
    10321 cttcttatat ttccgttttc attatatgtt gaataaattg catctaagca atctaagatg
    10381 taatttatat cacaattttt agggtcttca ccagcttcta gagcaagtct tttatgaccc
    10441 attttttcta atattttaac ctcttctttt atctcttcca tggtaagttt ttttctttta
    10501 aatttgttgc atttactaaa accgcaataa acacattcat ttacacagta gtttgataca
    10561 taaagagggg caaataaaac cactctatta ccatatattt cattttttat ttgcccagct
    10621 atactaaata atctatctag ttgtttttta tcttccactt caagaagtgt agctatatct
    10681 ttataactaa gtttttccct tctatcagcc ttatcaagta ccttttcaat atcatcagaa
    10741 gttgaatttt ttgcatcttc taataaagaa ttaataagtt catggtttat gaacatttac
    10801 aaacgcctcc attcaatatt atcaccgcga gaatagtcaa cttcaagtcc taatgacttg
    10861 atgttttctt ctaaactttg gtgatattca caagcttctt gaccagtaaa ggctttgtta
    10921 tcgtaaagag aatattgttt tctaaattct tgaggagaaa ggtttggcat aattacattg
    10981 caaccagcta aaagacctgc atttctacca ctagggttta tagatgccaa ggctgttgta
    11041 gcgggtaata aaacttttgg aagtagaagt cttgtaattg aaagcataag taatgtcttc
    11101 tctaaatctc catgtttata atccttaaaa attgtatcat ggtgtggaat gaaaggtcct
    11161 attccaacca tatgaggatt taactctttt aggtataata aatcacgaac aaggtcttcg
    11221 ttgttctgaa atggtgattc aaccataaat ccagcaccta cttgataacc aatttcttta
    11281 aggtttttta ggcattcttt tctagttcta agctcaatgt ttggcggatg taatttttta
    11341 taatgactgt ctgtagcagt ttcatgtctt aaaagatatc tatctgcacc acataaaaag
    11401 tattttttat acgaatcata actcttttct cctagtgata gagttatggc acaatcctca
    11461 tatttatttt ttatacttga aacaatttca catattttat catcagtaaa atatgcatct
    11521 tcaccacctt gcaaaacgaa tgttctgtat cctattttat aacctatatc acaacattca
    11581 agtatttcat caagtgaaag cctgtatctt atgatatttt tattagaaga ttgtattcca
    11641 caatagtagc aattattttt acaatagttt gtaagctcta taagtcctct caagtagact
    11701 ctatttccat aatgtttatt tctcatgtcg ctagcttgtt tataaagata gtttcttaaa
    11761 ttttgttcga agttatattc ctcattgtcg atattttcta ttaaatatag tagttcatca
    11821 aaacttaatg aattaatgct gtaaagttta tctataaagt atttaatctt atttttagtc
    11881 atattcaaat atgtaattat taagtatagt taaattttta ataattgaaa agttcaagtg
    11941 atctttccaa aatacctttt acatatgcta ttaagacacc atagtttaca ataggtacat
    12001 tgaaagattt tgccttttct attctagata acattccagc tctattcatc atacaagctc
    12061 cacagtgaac tacaagggca tatttattta tatcttcagt gaaggatact ccagaactaa
    12121 attcaaaagt aatgtttttg ccagttttct ttctaatcat attaggaatt tttactgttc
    12181 caatatcatc agtttgtcta tgatgggtac atccttcggc catcaagatt ttatcaccat
    12241 cttttaaatt ttctaaagca taagctccat taatcaattc tttaaggtct cccttttgtc
    12301 tagcaaacaa tatagaaaaa gatgtaagag gaatatcttt tggtgtatct ttatctactt
    12361 gtggaaatac ttgagagtca gtaataacaa gttttggttt tttgcctaaa ttagatagag
    12421 tttcttttaa actatcttct tttgtaacta tagaaattgc accattatcc aaaatatctc
    12481 ttataacttg ctgttgagga agtataagtc taccttttgg agctgcttta tctattggaa
    12541 caactaaaac aactaaatca tttggttcaa ttaaatctga taccaatttg aactcagtgc
    12601 tatccttagg caaaactttt attatttcat tttttaaatt ttcaattcca attttatctg
    12661 ttgatgagac agaaacaact ggacatttaa ctttattttt agttaagttt aaaatttctg
    12721 attgattctt aatagtatca attttgttaa gaattagaat atgagggata ttcttatcat
    12781 taaatttttc aattagtgac aaatcttctt gtgatatacc aatttggcaa tccactacta
    12841 gtaaagctat atctgtcttt tctaaaacat ctagtgattt acctattcta agttcaccaa
    12901 gttcaccaac atcatcaaga ccagcagtat ctataagaac acaaggtcct ataggaagga
    12961 tttccattgg tctaaatact gggtctgtag tagttccagc aatatctgag actattgcag
    13021 cactttgatt tgttatagca tttattatgc tagatttacc tgcatttctt ttaccaaaga
    13081 gacctatatg tactcttacc gattgtggag tagaatttag actcatttgt actcaccacc
    13141 ttaaacttct ttttttgata tactagtttt tacagataca tgaggtatgt tgcctaattt
    13201 accagtcaaa ctattaatag tatccatttc gccaacaact gctatacata cgattgagac
    13261 accttcttct tcgcaaggaa tgcccattct tcctttaata atacctttaa aacctgatac
    13321 aacctcattg aatttaaatt gacattcttt tggctcttcc aaaatagcac ttataactgc
    13381 aacctttttc ataaatatcc tcctaactca gaaaatatgt cttaaacaaa tccaaaatca
    13441 aaaaactttt ctttgtaaag aaaagtttct acaaaagaaa agttattaat tacacaagta
    13501 taatgtgcta tattaaacta tttccttaca gattagataa ctcttttctg gtagggttag
    13561 cttgtaaaaa tttaatttaa caacaagtaa ctgaagaaaa tttttataca atttatataa
    13621 attataataa aaatta
//
LOCUS       DAGSRM010000105        13219 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.105, whole genome shotgun sequence.
ACCESSION   DAGSRM010000105 DAGSRM010000000
VERSION     DAGSRM010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13219
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.105"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(6..1364)
                     /locus_tag="MJE33_003125"
     CDS             complement(6..1364)
                     /locus_tag="MJE33_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBY9649044.1"
                     /translation="MKAKSTSKFELDGIPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     AAGLNHKMSTLLIQAALVFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVASKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNPINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLAFGLDIRLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKVVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLEKFPKFIETLLADSSVVSGAMVAIIIQALYMVKFPGNKSDKVEDKNK
                     SL"
     gene            complement(2710..3675)
                     /locus_tag="MJE33_003126"
     CDS             complement(2710..3675)
                     /locus_tag="MJE33_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649045.1"
                     /translation="MVMQVQEKILELLYTINEDVVSIKNDMSEVKQEINVLKEDMSEV
                     KQEITVMKEDMSEVKQEINVLKGNMSEVKQEMIVMKEDMSEVRQEINIMKEDMSEVRQ
                     EINTMKEDMSEVRQEMTVMKEDMSEVKQEINVLKEDMSEVRQEINIMKEDMSEVRQEM
                     TVMKEDTSEVKQEINVMKKDMSEVRQEITVMKEDVSEVKSEVNFMQNKINNINEDMNG
                     IKDEVSIANEKLYGIEIKIDSLECEDKSMKEIQIEQNNILGSLLHNSEINKATHDNIE
                     HNIAYIKGDTNSIKEDIAEIRRDLNLVELATSKNWSDIVKLKSVK"
     gene            complement(3905..4990)
                     /locus_tag="MJE33_003127"
     CDS             complement(3905..4990)
                     /locus_tag="MJE33_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBY9649046.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTANALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASIGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKERVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            complement(5026..6339)
                     /locus_tag="MJE33_003128"
     CDS             complement(5026..6339)
                     /locus_tag="MJE33_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBY9649047.1"
                     /translation="MIAIKSKYLISSADEIYANSAVVVENDIIKDILPNEEVEIKYKN
                     IEEIIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAILSLESCE
                     RISFENGLRCLDENSNLIKWSRENSKLINGIMCTHTSFTCSDRFIKKAKEDAKKLNSP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDETILASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATEHGAYVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKKAIVKEMKEVACEFWKF"
     gene            complement(6340..8631)
                     /locus_tag="MJE33_003129"
     CDS             complement(6340..8631)
                     /locus_tag="MJE33_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBY9649048.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDIKNALKVKGVVNIFTYKDVPNYRYSMVGESYPEASPHDQLILEDVVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSKALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGADKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGRTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVRDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKQKILKVAQKKLDLPIDDLI
                     LYEDRVCYSEDVEKFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFAEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            complement(8621..9415)
                     /locus_tag="MJE33_003130"
     CDS             complement(8621..9415)
                     /locus_tag="MJE33_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBY9649049.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKDLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMNLADFLESELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMAKALVVRMYNSIGGECDGK"
     gene            complement(9454..9900)
                     /locus_tag="MJE33_003131"
     CDS             complement(9454..9900)
                     /locus_tag="MJE33_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBY9649050.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVDGQHITTLEGVLDEAKKFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            complement(9972..11378)
                     /locus_tag="MJE33_003132"
     CDS             complement(9972..11378)
                     /locus_tag="MJE33_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBY9649051.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHNHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VVKEYDDALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTEKD
                     MELTKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSKILTANEEKALKDAQDTAERLC
                     VRGTIKNRMEGHKWNSLY"
     gene            complement(11396..12766)
                     /gene="hydA"
                     /locus_tag="MJE33_003133"
     CDS             complement(11396..12766)
                     /gene="hydA"
                     /locus_tag="MJE33_003133"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBY9649052.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDEKIVEIGNNLEINGDKVID
                     ATGKIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGEDLL
                     QGVEAYHKKASGNCYCDYGFHMTITCLNKDTFNHMEKLISDGIVSFKMYMAYDGMKVD
                     DGTIYRVLKKARDLGCIVGFHCENGDLLDVLIEENIQNGNLEPKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCRESLETVKMAREKNIDMIVETCPQYLLLEDNLYNKD
                     KFEGAKYVMSPPLRKKEDINYLWEGLSSGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISVNKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNINKEETISYKTQKQNVDYTPYEGFKVKCKVEDVFLRGNHVVQSCNVKEHPTGQYI
                     KRKITK"
     gene            complement(12899..>13219)
                     /locus_tag="MJE33_003134"
     CDS             complement(12899..>13219)
                     /locus_tag="MJE33_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBY9649053.1"
                     /translation="VEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDS
                     EITQYSFCTNGSHYAGEAGIKTIGFGPSKENLAHTIDEYIEQEQLFIGTEGYYGILKS
                     VYGK"
BASE COUNT         3988 a         2606 c         1402 g         5223 t
ORIGIN      
        1 ttacattata aacttttatt tttatcctca accttatcac ttttatttcc aggaaatttt
       61 accatatata aagcttgaat tattatggct accattgctc ctgaaactac acttgaatct
      121 gcaagtaatg tctctataaa ttttggaaat ttttcaagaa cttgaggagc taccatgaat
      181 ccaataccag aagccataga aagacctaat atcatgctat tttcttgatt aaatccatcc
      241 atactaacaa gtcttaaacc agatgttgta agagttgaaa atataacgag tgttgctcct
      301 ccaaccacac agggaggaat tgttcttatt aaattagcta ataaaggcga tattcctgcc
      361 aaaaataatc caattcctcc aatagctagt acaaatttac ttacaacctt tgtattggaa
      421 acgattgcag aattttgtcc aaacatcccc attggagttg cagaaaataa tgcacctatt
      481 gtagagagta ctccatttcc taaaacagca gaagaaagtt cttcatctgt tacttgtcta
      541 ttaagccctc ctaccgcaga aagtgtacaa gcacccatta tatcagctat ctcaactaaa
      601 tatattattg aaaacattac aacaacttca agtcttatat ctagcccaaa agcaagtggt
      661 tttggaagtg caaccaaagt aaactcctga attgaagaaa aatttactat acccaaaatt
      721 aatgataaaa tgtatccaaa aattatgcta actaatattg atgcatcttt aaataatcct
      781 tttccatact tatttaatcc taatatcata agagatacag atattccaac tataaaatta
      841 attggattcc caaaagtctc attgcccata cccccagcta agttttttat agctattgga
      901 aaaagtccaa gacccataca tgcaactatt gttcctgata cagctggagt aaatataaat
      961 cgtatttttt taagaccaaa accaattata attattacta taccagctat tatttgagag
     1021 ccaaataata ctggtagtcc atacttactt gcaacagata ccccagctcc tataaacata
     1081 gctgtagctc ccattataac tggtattcct cctcctaatt taaaaccttt aaacaaaggt
     1141 attggatata attgtaataa agttgataat cctgcaaaaa ctaatgcggc ttgaatcaat
     1201 aatgttgaca tcttatgatt aagtccagct gcatttgcaa tcaatatcgc tggtaaacaa
     1261 ctacctatta ccattgccat taagtgttgt aaccctaaag gtattgcttc ctttagtggt
     1321 ggtattccgt ctaattcaaa ttttgatgta ctttttgctt tcataataaa aactcccgtt
     1381 acataaaact tattgaatta tataaatact taaaaatcta atatattaaa ttaatgatgt
     1441 tcataaatat caactatact ccaacatata tttaagtttt tagtcttaaa aaacagatgc
     1501 aataaattta attattaaca aatacacgta ttctttaatt ttgtatacat tatataccat
     1561 gctttacaaa tataagagta ttatagctat gaatttttaa ttttttatag taaatttaat
     1621 tcaatttaaa tctttcaatc ttaatcatta taagttataa gttttcataa gtatttacat
     1681 gtttcaagtt ttaaacattt acctataaac gctactttat agatgtcact tcaaatccag
     1741 tgacctcata ctttcatatg agcgtagact atttcttcat cttcagcatt acctgctaag
     1801 agcaacccgt ttccactatc aatcgcttat agtgtactct ctctcgagat agtcgtttga
     1861 ccttcctatt tctaggcttg gcgaccaaat gtccattttt aagtacttag gctctcacca
     1921 tataccatcc tcactattgt ttctggtttt caccacattc ataaaagttt ctttctatct
     1981 tttattgtag ttgcaaggct ttaggattta ctggttttaa agttgtgtcc tacatagatt
     2041 tctctatgta cggggccaaa agtacaattt atttaacata ctttgcttta ccccctgtaa
     2101 ataatattat ttactctttc actgtttttg ttattaaatt aattaaatat caattaatct
     2161 aagcataaaa taattattta atttaaactc aaaatattta tgaatatttt ataatataat
     2221 tctcataaga cagaaaaact taataagcta tgtatctcag ttgtaccaat gtactctcct
     2281 ttactatatt tgtatctgca acataaaata tacaagtttc tatctattta tagatatata
     2341 tatctataag caaaatagta atattatttt tatagtaaaa attttattca tattactatt
     2401 tataattttt atagagcaat tttagtgcca atatttgcat acatttgtat agtataaaat
     2461 ttttatgttc tcattgataa aattttactt atttcaagcg ttctattaga ttttatcatc
     2521 taaattttag gttttatttt tataaattaa ttgtatttac ataaaaaaaa agacctaaaa
     2581 caaattttta acggtctttt ctcaattttt tattttcatt tctaaaattt ataataaata
     2641 gtaagaacac tataaattct tttatataaa attcttagtt tgtggatgat aaattagtga
     2701 attaaaatac tactttactg attttagttt gacaatatca ctccaatttt tactagtggc
     2761 taactctact aaatttaagt ctcttcttat ttcagctata tcctctttta tagaattagt
     2821 gtcacctttt atatatgcta tgttatgttc tatattatca tgagtagctt tatttatttc
     2881 agaattatgt aataaacttc ctaaaatatt attctgttct atctgtatct ctttcattga
     2941 tttatcttca cattctagac tatcaatctt aatttcaatt ccatataatt tttcatttgc
     3001 tatggatacc tcgtccttaa ttccattcat atcttcatta atgttattta ttttattttg
     3061 cataaagttt acttctgatt taacttcgga aacatcttct ttcattacag ttatttcttg
     3121 tctcacttct gacatgtctt ttttcattac atttatctct tgttttacct ctgatgtatc
     3181 ttctttcatt acggtcattt cttgtcttac ttcggacata tcttctttca ttatatttat
     3241 ctcttgtctt acttcggaca tatcttcttt taatacattt atctcctgtt ttacttccga
     3301 catatcttct ttcattacag tcatttcttg tcttacttcc gacatatcct ctttcattgt
     3361 atttatctct tgtcttacct cggacatatc ctctttcatt atatttatct cttgtcttac
     3421 ctcggacata tcttctttca ttacaatcat ttcctgtttt acttctgaca tatttccttt
     3481 taatacattt atttcctgtt ttacttccga catatcttct ttcattacag ttatttcttg
     3541 ttttacttcc gacatatctt cttttaacac atttatttct tgttttactt ccgacatatc
     3601 attttttatg ctaactacat cttcatttat agtatataat aactctaaaa tcttctcttg
     3661 cacttgcatc accatcccta ttaattatta taacatttta cacaaatgct atacaatgtt
     3721 aacatatata tcagaattat taaaatttta attaaacaaa attgattcac tttattactt
     3781 caaaactaat taatatctaa aataactaca aataaacaaa aataaggtca acaacaaaaa
     3841 aggcttatct ctttgagata gcctttctta tttttattta aaactttgtt ttttataaaa
     3901 taatctatac attcagctct gaacatgaaa tttttcttcc aagtgctttt ggtttacaaa
     3961 ctgatataca aagtccacaa tatctacact cttcttcatt caattccatc ttatcattca
     4021 attttctcgc ttgatatgca caaactctct cgcacatctt acatttagta catttttctt
     4081 catcataagt aaactcaaag ctaagtcttt cattaacatc tcccatatga ttatgtacta
     4141 taccacaagc attttctata gtattatatc ctaatctatt aagattagat tttaaatcat
     4201 catgtatttt agatattact tgtgcgcctt ttaaaattac tactgaacat ataccaacaa
     4261 agtcagcacc tgccataatc atttctaagg catcatctcc acttacacat ccacctaagc
     4321 caattattgg tatattaact tgttttttta tttcatatat tctctgtaaa gttattggct
     4381 tgatagtttc tccacttaaa tatcctattc catttccacc taataaaggt tgccctgttt
     4441 ctatgtctat tctaaatgct ggtcctacag agtcacaagc agttattgca tctgctcctg
     4501 cttcttctaa ttttttagca aaatcgccta tctcatctat cataggagga agtttaacta
     4561 ttacaggtat atttactctt tcctttgcat ctttaagcat tggtatcaaa tctctatagt
     4621 cataagatac taattcaata aagtcagctc ctgcatttgc aactttctct actaattcgc
     4681 tactttcttc aattgtatga cctattgaag ctatacagac agtcccatct cttttcattt
     4741 gtggtatttc aaaatctatc cattgctctg gctcaaaatc tgtccatttt tcattattta
     4801 aaagagcatt agctgtattt ctaaccatat gtggtgctgg atttatagct ctttcttttc
     4861 ttatagtctt agtcacaact gcccctgctc ctgcatcact taatatcttg catccagctg
     4921 cactatatgt taatggtcct gaacctatta taagtggtga ttttaattct ttacctaaaa
     4981 aattatacat tatctgcttc cccttttcat atatatttat attcgttaaa atttccaaaa
     5041 ttcacaagca acttctttca tttctttaac aatagctttt ttatctaaat caactaactg
     5101 tttttctttt aaaatatgtc taccatctat gtatacattt gatataaatt cttttttacc
     5161 ctgaactact atctggtcaa atatattatc aagagtcaca ggagttttaa attcatcttc
     5221 cataactatt atatcagcct tattaccaac tgctatttta ccagaatttt gaagtcctaa
     5281 tacataagca ccatgctctg ttgccattct aaatactaaa tttgctggca ttactgatgc
     5341 atcttcttca actgatttat gcattaaaaa tgcacctctc ataacttcaa aaaaatcatt
     5401 tatatatcca tcagtaccca gtgccacttt tatgcctttt ttaatcattt ttggtactgg
     5461 agaaaaacct ccacctactt cacagttgct aagaggcata tgaactactt ttacaccttt
     5521 ttcttttaat atatctattt cttcatcatt tacttttaca cactgagatg ctaatatagt
     5581 ttcatccaat atatctaaat cattatagta gtcaactgct tttttgccaa aatgtttttc
     5641 agcatagttt ggctcatata tactctcaca taaatgaaat tgcattggtg aatttaattt
     5701 ttttgcatct tctttagctt tcttaataaa tctatcagaa catgtgaagc ttgtatgtgt
     5761 acacattatt ccatttatta atttactatt ttctctactc cactttatta aattagaatt
     5821 ttcatctaaa catctaagtc cattttcaaa gcttattctt tcacaacttt ctaaggataa
     5881 aatcgctttc atgcctattt cttctagtat ttttccttgc tcaattaaag ttccttcttc
     5941 tgtatttgga gcttctaggg tatcacaaaa agctaccaca ccagattcta taagttctat
     6001 tgctgatgct ttagttgttg ccttcacttc tttcaatcca attctatttt ctataaatgg
     6061 ccaccaatag tcatttaaaa aaccttcaaa gtcagtaaaa tgtacatttg ccggtattcc
     6121 tctagaaaga actccatatt gatgcatatg accattgacg aatcctggca ttattatagc
     6181 atctgattta tctattattt cttcaatatt tttatatttt atttcaactt cttcatttgg
     6241 caatatatct tttataatgt cattttctac tacaacagca gaatttgcat atatttcatc
     6301 agcagaagat attaagtatt tacttttaat agcaatcatt tatttacctc cctgatttaa
     6361 ttttttcatg gccatataaa ctttctcaga agttatagga agttctctta tacgaactcc
     6421 tgtggcattg tatattgcat ttgctattgc tggtgcaggt gtatgaataa ctatctctcc
     6481 taaagattta gctccatatg gacctgttgg ctcataatta tctacaaaat caacaattat
     6541 gtttcctata tctttttttg ttggaacttt gtattgtaat agattagaag tttgaagctt
     6601 tccatcagaa ccatgtctag ggtcttcata cattgcaagc cctatacctt gagttacacc
     6661 accttcaact tgtattctag caagtgcagg attcataact gtaccacaat ctgtagaaca
     6721 agcataattt attactttaa actcccctgt tgttttatct aactctattt cagcaaatcc
     6781 tgctaagaat ggtttagcac tctcttgtat accataagat tcagatgaat ataaaacttc
     6841 ttgatttcca ccaccaacag attcttttcc taattcactt aacaaaacaa acttctctac
     6901 atcttcacta taacaaactc tgtcttcata tagtattaaa tcatctattg gtaaatcgag
     6961 tttcttctgt gctactttta atatcttttg ttttaaactt tcagcagatt ttattgtagc
     7021 atttccagtt acataagtag tacaagaagc gtatgcacca gtatcatatg gacacatatc
     7081 tgtatctcct gtataaacgg aaattctatc aatagatgta ttcaatgctt ttgcagctat
     7141 ttgtgcaagt attgtgtcag aaccagttcc taagtctgaa gacccagaaa acaacttata
     7201 agttccatct tcatccatcc tcatatttac tattgccatg tctatgttgg ctataccaga
     7261 accatgagtt cccacagaca ttccaacagc cctcacctta ttgttgccaa tatccctaac
     7321 agggtacttt tcatcccatc caataagttt tttacctcta attatacatt catcaagtgc
     7381 acaacttctt attggaaaat tcattattcc gccttcagtt tgttttttta taatattctt
     7441 taactttatt tctgttgggt ccatattaag tttatgcgct aattcatcaa ttgcacaatc
     7501 aagtgcaaat gtaccttgtg ttgctccata tcctcttaat gctcctcctg gaactaaatt
     7561 agtatataca gttctcccat caaattttat tgcaggtaca ttattatatg ttggaagtac
     7621 attatgacct gattcagagc aaactgaagg tccattatca ccataagcgc ctgtattgtt
     7681 caatgctttc atatctatag cttttatgtt tccttcttta tctgccccaa tttttatatc
     7741 aaaaaacatc tgatgtctag tattagtcat tgcaaaagtt tcttctctag tataaattag
     7801 ttttgctggt ctttttgtca tccaagttac aaatgatgca tatatctctg ttacagctat
     7861 gttttttcca ccaaaacctc cacctattct tggttttatt actctcactt ttgaaagagg
     7921 tattcccagt gctttagaaa cctgtcttct catatgataa gctgattggt ttgcagaagt
     7981 tactactaat ctgccattcg tatctatata cgtataagct ctatgagttt ccatcataca
     8041 atgggcttgt gattgagtca tataagaatg ttctactatt acatcacttt ctttaaactc
     8101 tttttctaca tcccctttac ccattttttc tctcgataca atattttttg tagcatccaa
     8161 tccaaaatca aatggacaaa acaaatcatc ttcttcatgg attaaaattt tatttccttc
     8221 cgattctctt gcatccaata taggagtcat tacttcatat tcaactttta taagtttcat
     8281 agctttaata gcagcccttt catcttcagc ggctattata gcaacttcat ctcctacata
     8341 tctaactacg tcttcaagta ttaattggtc atgaggtgat gcttctggat atgactctcc
     8401 aaccattgaa tatctataat ttggaacatc tttgtaagtg aatatgttta ctacaccttt
     8461 tacctttaat gcatttttta tatctatgtt ttttatcttc gcaaaagcat gtggacttct
     8521 aagaagctta attgttaaaa catctttatg aaaaattaaa tcttctgtat atattggttt
     8581 tccagtaaca atggcttcac tatctatttt tcttacctct ttatttacca tcacactcac
     8641 ctccaatact attatacatt ctcactacta aggcttttgc catatctttt ctgtattctt
     8701 tacttcctct tatattactt ccaaatgaaa gttcttcact tgatacttca ccagcttttt
     8761 ctatatcatt ttctaaactt aatatttcac ttgctttata ggcaattttt gctcttcttg
     8821 gtctagcgcc tattgcaatt ttataaatac ttccttcttt taacattgct ccatttaata
     8881 ctgcaaaatc acctgtacat tttcttatag aatcaaatac acctatagca tcttttttag
     8941 gtaatataac ttctattaat atatcttttt caagctcact ttctaaaaaa tcagctaagt
     9001 tcataacccc ttttttatat aacttaacct ttgcatctac cactagtaat gaaggtatta
     9061 aatctgaaaa tccatactta gcaaatacgc tagctcctac tctagctcca cttctaaatt
     9121 gaactcctac tatattagaa acagcgtttg aaattacccc attacagtaa tttttaataa
     9181 ccttatttaa ttctaaagtt cttaaacttg tgtctgctcc aattaatatg ctatcatcag
     9241 tttcatttat ataatctaaa gctaagtctt ttaaatctat tgctgatcca atatttttac
     9301 tacctagttt taaaaatcca catcctccaa gtattatgtt gttttttctt gatattagca
     9361 gttcataagc ttcatctaaa ctttttggaa ctacatattc ctttatagtt atcatactct
     9421 ctccccatat caacttaatt ttcacttaaa agtctaagtg tttaaatatt tatgtattgc
     9481 tcttaattga cctttatatc ctgtacatct acaaaggtta ccatttacat aatctataac
     9541 ttcttcttct gttggatttt gaagctctct tttcattgcc aaaaccatca ttataaatcc
     9601 aggagcacaa aatccacact ggtcagcccc ttcgtctgca agaaaatctc caaacttctt
     9661 tgcttcatcc aagacaccct caagtgttgt aatatgctga ccgtctactc tagcagtaag
     9721 ataattacat gaaagcacgg ccttgtcatt taccatcact gtacataacc cacatgagcc
     9781 tgtatcacac ccttttttaa cacttacata tccattttct cttaatgtat ctgctaaaaa
     9841 atcatctggt cttatactta attctttttc tttattattt atggtacatt tgataatcaa
     9901 ataaatcacc tcaaaactca tatttttaag ctaaagtttt actatacttt tctatgaata
     9961 aattacattt attaataaag gctattccat ttatgacctt ccattctatt ttttatagtt
    10021 cctcttacac aaagtctctc agctgtatct tgagcatctt ttaatgcttt ctcttcatta
    10081 gctgtcaata tttttgaatc ttccattatt atatttccat ctactataac actctctata
    10141 tttttcatac tagaagaata tactaatgta gatactggat tatgcatagg tatcgctttt
    10201 ggtgaaagca ttggattaaa tattagtaag tctgcttttt tacctatctc caatgaacct
    10261 atctcatctt ccattccaat agctcttgct ccatctatag ttgccaactc aagaactttt
    10321 tctgctgaca ttgctaatgg gtcacactta ttaactttat gttgaagtgc tgttaatttc
    10381 ataagttcaa gcatatcttg agagttatta ctagcggctc catctactcc taaactaact
    10441 gttattcctt ttttaagcat ttctggcact ggtgcaacac ctgaagataa gtacatatta
    10501 cttgctgtat tatgagacac tttcatatcg tattttttag taagttccat atctttttca
    10561 gttagataca cacaatgtac cataagcaca tttggtccaa gtattcctaa tttttctaaa
    10621 acatctatgt catattgccc atgcaattct tttgctgctt ctctatcaaa aggagtctct
    10681 gatatatgta ctgtaaataa agcatcatca tattctttta ctactctcca tagcatttca
    10741 agcatttctt gagaatttga ccatattgct gcaggagcta ctcctatttt gattcttcca
    10801 ttttctgtat tatggtgttt ttcaaacaat cttcttacat ctttttcaac cgtttctaca
    10861 tcttgcatta ttccaggatg aactccaaat tgagctcctg tattcataca tcctcttcca
    10921 agtattcctc ttattcctaa ttctttataa gcatctatta ttccatcaca aagacctggc
    10981 ttactatgag gatacatgta gtcaaccatt gttgttatac cacttctaag tccttcaata
    11041 catccaagca ttgcagcatc ataagtatct ttgggctcta aatagttagc tgctggaaat
    11101 gtcatagttt ctaaccagtc tttaagtacc atatcatctc ccaatccttt taataaagtt
    11161 tggaataaat gattatgtgt atttataaaa cctgggaata aaactttacc tttagcatct
    11221 ataatttttt taacatcagt atatttactt tctatctctt tagaatttcc aatatcagca
    11281 atcttgttat cttttactac taatgcccca tcaaaaataa cttctctctc tttattcaca
    11341 gttactatta tggcattctt aataagtata tccatcattc ttatttcccc ccgcattatt
    11401 tagtaatttt tcttttaata tattgacctg ttggatgttc tttaacatta caactttgta
    11461 ctacatgatt tcctctcaaa aatacatctt caactttaca tttaacctta aatccttcat
    11521 atggagtata atctacattt tgtttttgag ttttataact aattgtttct tctttattga
    11581 tatttaaaat gactatatct gcatcacttc ctacagctat ctcacctttt ttaggataca
    11641 ttccaaatat ttttgcagca ttcgtagaag taacttccac aaacttattt acacttattc
    11701 tattttctaa gaccccatat gtatataaaa gtgctaacct atgttcaaca ccaggtgctc
    11761 catttggaat cttactaaaa tcatcaattc ccaagtcttt ttgcccctta aaattaaatg
    11821 aacagtggtc tgtgccaact gtttgaatat ctccactaga caatccttcc caaaggtaat
    11881 ttatgtcttc tttttttctt aaaggaggag acattacata cttagcaccc tcaaatttat
    11941 ccttattata taaattgtct tctaataata gatactgtgg gcatgtttct actatcatat
    12001 ctatattttt ttctctagcc atttttacag tttcaagtga ctccctacaa cttaagtgga
    12061 ctacatagga ttttgaatta gataatttag ttatatcagc aagtcttgaa acagactctt
    12121 tctcaactat gttaggtctt gtaagtggat gatattttgg ctctaaatta ccattttgta
    12181 tattttcttc tatcaataca tctagtaaat ccccattttc acaatgaaat ccaactatac
    12241 aacctaaatc ccttgctttc tttaaaaccc tgtatattgt tccatcatct actttcatgc
    12301 catcatatgc catatacatc ttaaaagata ctatcccatc cgaaataagt ttttccatat
    12361 gattaaatgt atctttattt aaacatgtaa ttgtcatatg aaaaccataa tcacaataac
    12421 aatttccact tgctttttta tgatatgctt ctacaccttg cagtaggtct tccccctcat
    12481 tggcttctgc aaaatctagt attgtagtag ttcctccagc tatagctgct cttgttcctg
    12541 tttcaaaatt atctgcagtt gtaatagaac ctgcattcat atcaaaatga gtatgtgtgt
    12601 ctattccacc aggaattaca attttacctg tagcatcaat aaccttatca ccatttattt
    12661 ctaagttatt tcctatttca actatttttt catcttctat ccttaaatca cttatataag
    12721 tcttatcaga ggtaattata gttccacctc ttagtattgt tcccatatat acctcctaac
    12781 ttttaagctt taaagtattt tctaagtatt gtaaaacttt atttaaaatt gtattttcta
    12841 aaggtgctat attcagcacc tctagactat atttaaataa tttattgtga actaaatatt
    12901 attttccgta tacagatttt aatattccat agtaaccttc tgttcctata aataactgtt
    12961 cttgctctat atactcatct attgtgtgag caagattttc cttagaagga ccaaatccta
    13021 tagtttttat tccagcttca cctgcatagt gacttccatt cgtacaaaat gagtattgag
    13081 ttatttctga atctattcca gcttctttta atcccttata tgctgcttga acaaattcat
    13141 cttcttcgtc atataaccaa cctgggaaga atctttcact ttctatagta tttcctgtat
    13201 agcaatcagc tttttcaac
//
LOCUS       DAGSRM010000106        13211 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.106, whole genome shotgun sequence.
ACCESSION   DAGSRM010000106 DAGSRM010000000
VERSION     DAGSRM010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13211
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.106"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(158..811)
                     /locus_tag="MJE33_003135"
     CDS             complement(158..811)
                     /locus_tag="MJE33_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY9649054.1"
                     /translation="MKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIGVSRTP
                     AREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQNLTESD
                     LVKMEELTEMMAVSIKYKNYNSYLKLSNEFHDIYITKSDNQVLVNLLNSLRYNFMSKS
                     YTSDNEDELYKMLTYSNNQHIEVVKSMRENDLENVERVLREHWKTIPIAEMKKEKLL"
     gene            complement(1056..2921)
                     /locus_tag="MJE33_003136"
     CDS             complement(1056..2921)
                     /locus_tag="MJE33_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HBY9649055.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISL
                     EINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
                     PKKNSFIYGNWEDTPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDN
                     DSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYRDVIKIRNKNKLLSSGNTEFIYTNNDSVFSFIRVNENNDRMLILVN
                     RSENVESISLCIESSFIEEIPIKYSLKNANSTIQIENNELKVDLDSKSFRIFRVN"
     gene            complement(2926..5346)
                     /locus_tag="MJE33_003137"
     CDS             complement(2926..5346)
                     /locus_tag="MJE33_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HBY9649056.1"
                     /translation="MVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVLCSLLKDE
                     IAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLNIEEEVREGLSELGINLDDLIE
                     AEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFVNGYQVEVPDNW
                     LTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDIPIIGYQN
                     QCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYPDDSNYAG
                     KLLRLKQEYFFVSAGIQDIIRKYKKNKLNINNLFDKVAIHINDTHPTLCIPELMRILL
                     DEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMMKELLPRIYMIIEEINRRYVE
                     ELNNKGYDQDRIKRMSIIDCDNINMANLCIVTSHSVNGVAKLHTHILETEVLKDFYQD
                     EPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKKDTLQLKNIEKFKNDSSVLQ
                     RLDDIKYNNKANLAKFIKDKYDLNVDPSSIFDVQVKRLHAYKRQLLNIFNVLHMYHEL
                     LDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLKVVFLENY
                     GVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEICEQVGKE
                     NMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEEGRSIYNS
                     LTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSDRTISEYA
                     KDIWFKRV"
     gene            complement(5360..6802)
                     /gene="glgA"
                     /locus_tag="MJE33_003138"
     CDS             complement(5360..6802)
                     /gene="glgA"
                     /locus_tag="MJE33_003138"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HBY9649057.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTDAVLEAISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQKYFDNGDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSID
                     KKVENKLRLQDILGLKKDSSIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            complement(6831..7961)
                     /gene="glgD"
                     /locus_tag="MJE33_003139"
     CDS             complement(6831..7961)
                     /gene="glgD"
                     /locus_tag="MJE33_003139"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HBY9649058.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGN
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSERKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            complement(7963..9123)
                     /locus_tag="MJE33_003140"
     CDS             complement(7963..9123)
                     /locus_tag="MJE33_003140"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HBY9649059.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKGSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNPEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
     gene            complement(9894..10937)
                     /locus_tag="MJE33_003141"
     CDS             complement(9894..10937)
                     /locus_tag="MJE33_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBY9649060.1"
                     /translation="MGTKIVLSIVLIVVVVAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHFLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPQDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQMATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEVLD
                     IKEVLNDNESLKK"
     gene            complement(10958..11404)
                     /locus_tag="MJE33_003142"
     CDS             complement(10958..11404)
                     /locus_tag="MJE33_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBY9649061.1"
                     /translation="MKLIWLIVAVLFGIAEMLTPSLTLIWFSVGAVILIFLSSFIESI
                     FLQILIFAVISIAMLVVATKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVDATVNVTNQ"
     gene            complement(11578..13158)
                     /locus_tag="MJE33_003143"
     CDS             complement(11578..13158)
                     /locus_tag="MJE33_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HBY9649062.1"
                     /translation="MLIGTSKLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIDMY
                     NSYTVVCGVGNNGGDGLGIARHLNTYGKEVNVFLVGNLEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGDENEVDKIIKVEKQNIIKNKNLTIFKDRVSKSDVVVDAIFGTG
                     LKRDITGIFKEVIDIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRG
                     FLNYDTSALIGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRRDKHGFKSDFGK
                     VSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEERL
                     NKLLNSSDAIGFGPGMGDNSQTFDKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWK
                     NRFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNLVVLLKGYQTVITDGKKTY
                     INPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDKLSKAMYTIN
                     ATDLISNISLTMKELLDI"
BASE COUNT         4177 a         2422 c         1266 g         5346 t
ORIGIN      
        1 tctataagtt cattgattta tatatgttaa tttaaataca ttattctatc gtagtgcctt
       61 agagttttta tttgtattta aagttaaatt tattcttgtt tttcctttaa tatttaatag
      121 taaaacaaca aaattataaa aaaaataaag agaaacttta taaaagcttc tcttttttca
      181 tctctgctat aggtatcgtt ttccaatgtt ctctcaaaac tctttcaaca ttttctaaat
      241 cattctccct catagatttt actacttcaa tatgttgatt attgctgtat gtcaacattt
      301 tatataactc atcttcatta tcactagtat atgactttga cataaaatta tatcttaaag
      361 agttcaatag atttactaac acctgattat ctgatttagt tatgtatata tcgtgaaatt
      421 cattacttaa ttttaaatag ctattgtaat ttttatactt tatagagacc gccatcattt
      481 ccgttaattc ttccattttt actaggtctg attcagttaa attttgtaat gctgaagaac
      541 cggctaacgc atccaatact ccaattacgg aataggtgtc taatttctcc ttttcctgaa
      601 attcttttac tacaaatccc ttattaggta ttttttctag taaattttca cctgctaacc
      661 tagttaatgc ttctctcgct ggtgttctac taactccgat agatttacaa atctcagatt
      721 cagtaattct ctgattagct tcatactctc cattttggat tttctttgat atatagtcat
      781 atacttgagt tgtaagtgga tctaccttca taagacgttc ctccatatta agattataaa
      841 agttaaatct ccctacaact ttggtagaga gttcagtttt atcttactaa tttatataat
      901 atcacaatta tgatattttt tatacttctt tgtaatattt tgaaatacta aaattcttat
      961 gataatttat acaaaaaaag tagaaaaagt tttgataaaa caaaactctt tccacattca
     1021 ccttattttc tatttatttt tctaaaaaaa gatatttaat ttactctaaa aattctaaat
     1081 gatttagagt ctaaatctac tttcaactca ttattttcaa tttgtatagt actatttgca
     1141 tttttaagac tatactttat aggtatttcc tcaataaaac tactctctat acataatgat
     1201 atactttcca cattttcact tctatttact aaaattaaca ttctatcatt attttcatta
     1261 actctaataa aagaaaatac agaatcatta ttagtatata taaattctgt atttccagaa
     1321 cttaaaagtt tattcttatt tctaattttt ataacatctc tataaaaatt aagcatatct
     1381 tcgtcttcat ttttccatgg ataagttctt ctattatctg ggtcaacacc tccacaaaga
     1441 cctgcttcat ctccataata tatatatggt actccttcaa atgtcatctg tatagctaca
     1501 gcaagcttca ctaaatctac atcatcattt aattccgttt tagctcttgt aacatcatgt
     1561 gttccaatta aatttaaatt agacctaaat gattcttttg gataattttc tttaatctgc
     1621 atatatctat tacaaagttc atatgaatta atttctcctt ttaagaaaga aaacatgtta
     1681 tttctaaaag gataacccat aacagaatct aattcttcac ctaataaata acttcttctc
     1741 tgaccatagc taatcttatt tgatgcatct tcccatactt cacctataag aattgagtca
     1801 tcatctgcct tttttaattc cttttttaac tctcttataa acttagttgg taattcgtct
     1861 gcaacatcta gtctccagcc cttaataccc atatttaccc atttatttat aacgctatca
     1921 ttgtcatata ttatataatc catatagcta tgttctaatt catttatatt tggcaatgtc
     1981 ttaacatccc accaactttt atatttttga ggaaattctt caaacatata ccatgaataa
     2041 tatggagatt ctttagattg atatgcccct aaactattat agtttccata catattaaaa
     2101 tatttactat ctgctccagt atgactaaaa actccatcta atactattga tatacctttt
     2161 tcttttgctt tatcaataag ttctttaaat gtatcttcat ctccaaacat tgggtcaatt
     2221 tttttatagt cacctgtatc atacttatga ttactagagg cttcaaatat agggcttaga
     2281 tatagtatac ttacacctaa tttttttaaa tatccaagtt tattgattat ccctcttaaa
     2341 tttccaccat ggaaatccca tcttataaca tccccttgac tatcctttat gtacataggt
     2401 gtatcttccc aatttccata tatgaaacta ttttttttag gattatcaac cttaccacta
     2461 cgatttccat tattgaatct atctacaaat atatgataaa gtattccttc tttgtaccat
     2521 gttggtactt taaaatcttt gtaaacagtg atttgatatt tattaatatc actataatta
     2581 tattcacaag aatatccatt atctctgttt tttccataaa ataaagtctt tatctgacca
     2641 tccatttcaa catcaacttt aaagtaataa aaataaacac ctgtaccatc aaacttttca
     2701 atcttaccac aaaagcactt tccaagttta tcattatcta attcttcatt taaagttatt
     2761 gttctcattt cttcttctct atttatttct agggaaatag acttgatatt gtatccctca
     2821 tttactttaa cacaaatggt caattcctca tcaaatttta aagctccaaa cggagcctta
     2881 aaatttttat cccaagagtt atattctatt acactttgca tttaatcaca cccttttaaa
     2941 ccaaatgtct tttgcatact cagaaattgt tctatcagaa gaaaaaaatc cagagttagc
     3001 tatattaact aaagacattt tattccattt ttctttatct ctatataacc tgtttatatc
     3061 tgcttgagct tgaccataat tttcaaaatc tctcaataca aaatactcat cattgtaagt
     3121 agtgagtgaa ttataaatac ttcttccttc ttcgccaaga ttaggaataa atccatttat
     3181 taaatcatct actacccttt ttatatccat attagaatgg tataggtcaa gtgaagaata
     3241 tccaccatat ttattataat ttagtacttg ttctgcacta agtccaaata aaaacatatt
     3301 ttctttacca acctgttcac atatctctac atttgcaccg tcaagtgtag ccaaagttat
     3361 agcaccattc atcataaact tcatattact tgttccagat gcttccttag ttgtagttga
     3421 aatttgctca ctaacatttg ctgctggtat tattatttct gctaatgata ctccataatt
     3481 ctctaaaaat actactttta acttgtcctt tactctaaca tcattattta tagtacttgc
     3541 aacagaattt ataaatttaa ttatacattt agccaaataa tatcctggtg ctgcttttgc
     3601 gccaaatata aaagttcttg ggtcaagatt caaattagga ttatcaagta attcatgata
     3661 catatgaagt acattaaata tattcagtaa ctgtctttta tacgcatgca atctcttaac
     3721 ttgaacatca aatatcgaac ttgggtctac atttaaatcg tatttatctt ttataaactt
     3781 tgctagattt gctttattat tgtattttat atcatcaagt ctttgtaaaa cagaggaatc
     3841 atttttaaat ttttcaatat tttttaattg taatgtatct ttcttccatg aatcaccaat
     3901 taaatctgta attaaattgc taagttgagg atttgaactt ataagccatc gcctatgtgc
     3961 aattccatta gtcttattat taaatttatt tggttcatct tgatagaaat cttttaatac
     4021 ctctgtttca agaatatgag tatgaagctt agcaactcca tttacactat gacttgtgac
     4081 aatacataag tttgccatat tgatattgtc acagtctatt atactcattc tttttattct
     4141 atcttggtca tatcctttat tatttaattc ctctacatat cttctattta tttcttctat
     4201 tatcatataa attcttggta atagttcttt catcatactt acaggccatt tttccattgc
     4261 ttctgacatt atagtatgat tagtatagga cactgttttt ttagttattt cccatgcttc
     4321 atcccaagat aaattttctt catcaagcaa aattctcata agttctggta tgcataaggt
     4381 aggatgtgta tcatttatat gaattgctac cttatcgaat aaattattaa tatttaattt
     4441 gttcttttta tattttctta ttatatcttg tatccctgca cttacaaaaa agtattcttg
     4501 tttcaacctc aacaacttac ctgcatagtt tgaatcatca ggatataata cctgtgatat
     4561 ttcctcagta taatacttat gttttaaagc ctcttcatag ctaccagaat aatttagagc
     4621 atttgaggta agttcaccaa aatctctctt tggtatttca ctcttaaaca atcttaaagt
     4681 atttatacat tggttttggt atccgattat tggtatatca taaggcattg ccattacagg
     4741 aaggtagttt ttatgaatta cttttaaatg actaccttcc ttgattaatt ctacctcacc
     4801 accaaacttc accattgttg cttcatttgg tcttacagtt tcccatgcat atcgtccttc
     4861 tgtaagccag ttatcaggca cttctacctg ataaccattt acaaattttt gttcaaacag
     4921 accatattta taccttattc catatccttg accacttatg ttcaaagatg ccatagaatc
     4981 taaaaagcaa gctgctaatc taccaagtcc accatttcct aaggctgggt ctacttcagc
     5041 ttcaattaaa tcatccaagt tgattcctaa ttcagataaa ccttctctaa cttcttcttc
     5101 aatatttaaa tttaataaat tagattttaa ctgtctccct attaaaaatt ctaaactaaa
     5161 ataataaact tcttttttct tatctaactt tgtagcaacc catttttttg ctatctcatc
     5221 tttcaataga ctacataata catttaacaa ttgttcatca gtagcttcct tgatagattg
     5281 tgcatataag ctatacattt ttacttcaaa tttttgcttg aatttctttt tgcttattgt
     5341 taccaaaaaa accactcctt taatcacata tgtctctata tgtttctata tactccttag
     5401 ctgatttttt ccaactatta tctgttttca ttgcattttc aactaattta atccattttt
     5461 ctttgtcctt aaatactttg attgcatttt tcaaacagaa aaacatctca tgagcattgt
     5521 aatttttaaa actaaatcca ttgccttctc cagtaaattt attataaggt gttactgtat
     5581 ctctaagccc tccagtctct ctaactattg gtaaagaacc atatctcata gctaacattt
     5641 gacctatacc acaaggctca aataatgatg gcattaagaa catatcactt gcagcatata
     5701 tctgttgagc caatgatgca ttaaaagtaa ttctagctga tactttatct gaaaaattag
     5761 aatcgtaata gttgaacatt gattgatatt gatgttctcc agttcctaaa acgacaatct
     5821 gtaaatcttc tctcataagt tctggcatca tgtatgctat taaatcaaat cctttttgag
     5881 aaactaatct cgaaactata cctatcaacg gaatagatga atctttttta agtcccaata
     5941 tatcttgtaa tctcaattta ttttctactt ttttgtcaat tgagtctaca tcataatgaa
     6001 caaatatatc tttatctttt gcagggtcat ttaaatcata atctatacca tttaatatac
     6061 cttttaattt tcctgactct tttctaagta atccatctaa ttgttcacca taaaaagatg
     6121 tttgtatttc atttgcataa gtaggactaa cagttataat tttatctgca aaatttatac
     6181 cagctttcat aaaatttatt gagccataat actctatatc tccattatca aagtattttt
     6241 gtggtaaatc caatatatca tataaaacat gcttatcaaa tacaccttga tattggagat
     6301 tatgtatagt atacatagtt tttatatttt tatacccctc aagggttgca tatctttctt
     6361 ttagaattaa tggtaacatc cctgtatgcc aatcatttat atgtattaca tctggataaa
     6421 aatcaattct acttatagct tctaaaactg catctgtaaa gaaagtatat ctttctgcat
     6481 catctccata tccatagata ctctttcttt catcttctct tctaaaataa aattcattat
     6541 ctataaagta aaattttaca ccatccaatt ccatttctaa aagaccacag tattgatttc
     6601 tccatccaac acgtacacta aacacagcta tttcttttag ttgatctttt aaataacttg
     6661 gtatagttga atatttgggc attattaccc ttacatctac accttctttt tttaattctt
     6721 taggcaatgc ataagaaaca tctcctaatc ctccagtttt tgcaaagggc caacactctg
     6781 ctgttacata aaaaaccttc attagtcatt cccccttcta aaaaatttat ttatatattt
     6841 gtattctttt ccactacaag aggatatttt tcatctcctc ttaattcttt cccatttgat
     6901 acactcgtat ttttatctaa tattgcatta tatagttttg catttgactt tattacacaa
     6961 ttttgcatta ctattgagtt ctctataact gttccttttt caacatttac ctttctaaat
     7021 atgatactat cttttacagt accttctatt aaacaaccag atgctataaa gctattttct
     7081 actttagcac tatctgtata aattgtaggt gattcattct tttcttttgt aaatatcttt
     7141 ctttcagaat ataataattc atctgcaact tctaaattta ataaatcttt gctcatttca
     7201 aaatatgatt caacagaatt tacacatttt agatatcctg tatattcata ggctcctatt
     7261 ttcatgtttt ctgcttcttc aataataaat tcttctaaat aacaataatt gcctttattt
     7321 acaatgttat atatacattc aacaaaatct tcacgcttca taatatatgt ctccatacat
     7381 atgtttgctc tagggaactt acctaaatta tttcctacat ttattaccct attgttacta
     7441 tctaaattta gagttaagtt accagcaaaa tctttatttg cattatttac atttttatat
     7501 attatagtta tatcattatg agatttttta tggtattcca atgcttgaga ataatttaaa
     7561 ttgcatagca tatagcttgg agctattaaa atatattctt cttcactctt ttctatataa
     7621 tctagattag ctaagatagt atatatatca ccttctctat gtgagtaagt atttctatac
     7681 ttagtgtttt ctggactaaa aattgatagg ccaccttttt ttctacttaa atcccaatct
     7741 ttaccactac ctatatggtc tgttaatgat ctgtattttt cttttgcgaa tattccaact
     7801 ttagtgattc ctgaatttat catattagat aaagcaaagt ctattattct atatcttcca
     7861 gctattggtg tagatgctat tggtcttcca tagttaagtt tgtttattgc tgggtctcct
     7921 tttttgttta aatttattat ccctaaacat tcatttctca tcttaaagac ctccttctat
     7981 taatacgcat ctaggttcga tattttcata ctcagaaact acatttattt cattatttac
     8041 attctttatc tgaacattat catttatagt tgcacactca cctatcatag ctttttctat
     8101 agttacattt tcacctatta caacatttgg cattattact gaatcaatta ctttacttcc
     8161 tttaccaaca acaactccat gtgataatac agagttacct acttcaccta aaattctaca
     8221 accatcagct atcattgatc tatgaacatt tgcattttta cctatatatt gtggaggcat
     8281 tgccatagta tttgtatata tcttccactt agggtctgct aaatcaagtg tttctggaga
     8341 ttttattata tccatattag catcccatag actttgtata gttcctacat ctctccagta
     8401 tcctttaaat ggataagcat acattccaac attatcttct aacattttag gtattaaatt
     8461 ttttccaaaa tcatcataat ttatttctgg atttttttct gcttccttaa aataatttct
     8521 caacattttc caatcaaata tgtatactcc catagatgcc aaattgctct taggttcact
     8581 tggtttttct tcaaattcat agatacttga atcttcattt gtattcatta tcccaaatct
     8641 tgaagcttca tcccaaggaa cttcaatcac tgctattgta gctttagagc ctttttcctt
     8701 atgaaatttt aacattttgc tataatccat cttgtatata tggtcaccag ataatatcaa
     8761 tacatattct ggattatagg tatctacaaa gtccatattt tgatatatcg catgtgctgt
     8821 accattatac caatttcctt ctttttcatt cataaatggt tgtaaaacat agacaccacc
     8881 atttattctg tccaaatccc aatgacttcc catgcctata tgtgaattta atatcaacgg
     8941 tctatattga gttaaaacac ctacagtatc aataccagag tttgaacaat tgcttaacac
     9001 aaaatctata attctatact tacctccaaa tgatacagct ggtttggcaa ttctttttgt
     9061 aaaaacacct aatcttgaac cttgccctcc tgccaaaatc atagctaaca tctctttttt
     9121 catttaatga cactcccctc atctagtaat taatatttat aattgcattt ttacttttat
     9181 aaactttatc agtcaataaa tgtcgtgtat aaatgttgct taaataatac cttaataaaa
     9241 ataaaataat atcaactata caattgatat aatgtatacc ccataatgga cttaccgacg
     9301 tatacattat tttccataag cactatagta tttattaata ttcaattatc ggttaaaaaa
     9361 taatacaaat tttattttat aaatataaaa acatttttta taaatctttt gtgtcatata
     9421 aacatgctta ttttttaatt tgttaaattc gtaatagcaa aatataatct aatatttgtt
     9481 aaataattat ccaaagaaaa tcaagtaatt attaatcaac tttatttttt aattgatact
     9541 tctttgtcta taatttaaac aaaaggatat aaacttttaa attatttatt agttttctta
     9601 gtattaatgt ttatattttt ataaatatat attaataata taaatagact aactaaaacc
     9661 atgcttattt gctatatgtt ttatataata ttatataaca ctttttacat tagtaaagga
     9721 taaattgtat aaagattctg ttaatttatt tttataattt catctttcta ataaaaacaa
     9781 agaagaagac ttaaaaattt ataagtcctc ttcttttcaa cgtgcatttt tgtgctatat
     9841 ttaattaatt cgacctttaa tttccatatc cttcctatga taacaaaact cttttatttt
     9901 tttaatgatt cattatcatt taaaacttct tttatatcaa gtacttcttt gttatcatca
     9961 ctcataactt cctttattcc tttgaatgtt cctaggaaat taactgcttc tgaaggtaaa
    10021 accagttttg ttgacttacc ttcagccatt ttttcaagag cttccataga ttttaatgct
    10081 agaatattat tgtctatatc agcctctttc attgctgaaa aaactaattt taaaccttct
    10141 gctgttgcca tttgagttct ttttatcatt tcagcttcac cttgtgcctt agcaatagct
    10201 gtctgtctta tagcttcagc ttcaccttct gccactaata tagcagattc tttttcacct
    10261 tctgctacac gtaccatagc ttcttttttg gcttctgctg ttaatatagc agattgtttt
    10321 tcaccttcag cttgaagtat tgcagctgac ttattacctt ctgcttgaag tattgcctct
    10381 cttctttctc tttctgctct catttgtttt tccattgcaa cttgaatatc ctgaggaggc
    10441 attatatttt ttaactctac tctatttact tttatacccc atttatctgt tgcttcatca
    10501 agaatagttc tcatttttac atttattata tctcttgatg ttaatgtttc atctaaatca
    10561 agttcaccaa ttatatttct tagtgttgta gctgttaaat tttcaatagc agctattgga
    10621 ttagctatct caaacacata tctaactggg tctgtaactt tataatatac aactgtatca
    10681 atctgcatag ttacattatc tttagttata actggttgag gtggaaaatc tattacaatt
    10741 tctctaaggt caattacata tgccatttta tctaaaaatg gtattaagaa atgtacacct
    10801 gtttcagcta ctttttgaaa cttaccaagt ctcattatta tacctacttt agattgtttt
    10861 ataactctta tacaagttaa acttattgct acgactacta ctattaatac aatacttaaa
    10921 acaattttag tacccatact ataaatcctc ctttattcta ttggtttgtc acattaactg
    10981 tggcgtctac ttttttaaca actaatttta ccccttcaat ttttaatact tctactgtag
    11041 aacctttttc tatcttttca ccatctatag aaatagccgt ccatgtttca tgttcaacta
    11101 caactagacc tgtgttattt ggagaaatct cttctgtaac aatacctttt ttagacatca
    11161 ttgcttgtaa attagtgtta gatttatagc ctttatcttt ttttactatt tttttagtag
    11221 ctacgactaa cattgctata gaaattactg caaatattaa aatttgtaag aaaatactct
    11281 ctataaagct acttaaaaat ataagtatta cagctcctac actaaaccat atcaatgtta
    11341 agcttggtgt taacatttct gctattccaa ataagactgc tactatcaac catattaact
    11401 tcatactatc taccctcttt tcatatgaat tataaaaccc tatataaatt atatgtttat
    11461 acaatgtttt tgaatataaa tttaaattac aaaacaataa aataaattaa ttaattaata
    11521 gtattatatc aacatacttt gttaaaattg ttacaaagta gtatcaaata tcaccaatta
    11581 tatatccaac aactctttca ttgttaatga aatgttactt attaaatccg tagcatttat
    11641 cgtatacatt gctttgctca atttatctcc tatatagcca tgtatatatg ctccactaac
    11701 tgtagcttcc aatatatcca taccttgacc tataaatgaa gtaatcattc caagcaaaca
    11761 atcacccatt ccaccagttg ccatacagct atttcctgta ggatttatat atgttttttt
    11821 accatctgtt atgacagttt gataaccttt taaaagcacc accagattat atttttgagc
    11881 aaattctttt gcaacatcta ctctatgttc tcttatatac ccaattgaat ctcctgtcaa
    11941 cctggccatt tctcccaaat gaggtgtaat tacaaatcta tttttccatt ctaaaaactt
    12001 ataacatctg tccttcataa cgttcaatcc atctgcatct aatactattg ggcaattaga
    12061 attttctact atcttaagta atttatcaaa tgtttgacta ttatctccca tacctggacc
    12121 aaaaccaatt gcatcactag aattaagtaa tttatttaat ctttcttctt caaaatttaa
    12181 agtcattgct tctgtaagtc taatagatac cttttcttgc acatcttcac tactaattaa
    12241 tgttacaagt ccactcccac tcttaacagc agattcagtt gctatgaaac ttgcaccata
    12301 aaatcctttt gaacctgcaa ctatagatac ttttccaaaa tcacttttaa aaccatgctt
    12361 atctcttctt ctaatattat tttttacaaa agtcttatca gttatgaact cttcatcatg
    12421 gtattttctt gtaataaaat ctggaacacc tatcttttct acaactacat cacctataag
    12481 tgcgctagtg tcatagttta gaaaacctct tttataaaac tcaaagctaa tcgttttgtt
    12541 tgctctaata caagttccta atacatttcc attatcgcta tctatacctg aaggaatatc
    12601 tatagaatac gtatttttgc tattatcgtt tactatgtca ataacttctt taaaaatacc
    12661 agttatatca cgttttaatc cagtcccaaa tatagcatct actacaacat cacttttaga
    12721 tactctatct ttaaaaattg ttaaattttt attttttata atattttgtt tctcaacttt
    12781 aataatttta tctacttcat tttcatctcc ataatttttt tcacttaaat catttttatt
    12841 aatatttata atattaattc ccatatttaa aagtacattg taatttattt tagagcattc
    12901 acttagtttt tcaagattac ctactaaaaa tacgtttact tcttttccat atgtatttag
    12961 atgtcttgct attccaagac catctcctcc attatttcca acaccacata caactgtata
    13021 actattatac atgtctatat ccatattttt cattgcagat ataaccgcat tttccatcaa
    13081 tacaatcatt ggaatcccta cttcttctac acatgcagta tcaacatttc tgcttaattt
    13141 agatgtacct attagcataa attatcactc ctataaagtt aaagacttct taaatactta
    13201 ccaaaaatat c
//
LOCUS       DAGSRM010000107        13103 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.107, whole genome shotgun sequence.
ACCESSION   DAGSRM010000107 DAGSRM010000000
VERSION     DAGSRM010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13103
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.107"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            142..1023
                     /locus_tag="MJE33_003144"
     CDS             142..1023
                     /locus_tag="MJE33_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBY9649063.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPAKSIDFKFHTGVTTDIIQGLKERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAAKDTIDLKETIPYPQIVFNQRSGLRYIIDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYL
                     PPAIKNFKNFVIKNAQL"
     gene            complement(1231..2094)
                     /locus_tag="MJE33_003145"
     CDS             complement(1231..2094)
                     /locus_tag="MJE33_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HBY9649064.1"
                     /translation="MLSSYVKRVSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEY
                     KYAVSQGDYVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDE
                     LPLFLSRLECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARI
                     VIESRDDVSNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDADYREGLEIA
                     DILCNPLSRARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPIV
                     EPVGPWLEKMR"
     gene            complement(2249..4183)
                     /locus_tag="MJE33_003146"
     CDS             complement(2249..4183)
                     /locus_tag="MJE33_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HBY9649065.1"
                     /translation="MKKSLKKYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKDVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKTINNKKYYLGTDGARVSGWKVIDGKTYYFNSDGVMQTGWQEKNGKKYYLGSDGLAV
                     TGWQEIDGNKYYFDKTGIMQTGWQQIDGKSYYLDKDGKMLTGSQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYDST
                     ILLTNTANLPTETVEEMKLLAPKTVILIGGENAISSKLEQEIKTTFNTETKRIAGQDR
                     YQTATRIAEELGNREEIKTAYMVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAINDSVIAKMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDTDLQAVLVSKSDALVDALSAGPLAVKLHSPIVLMDNSGLSSEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILAK"
     gene            complement(4295..5308)
                     /gene="galE"
                     /locus_tag="MJE33_003147"
     CDS             complement(4295..5308)
                     /gene="galE"
                     /locus_tag="MJE33_003147"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBY9649066.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNSIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHVVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLAAGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            complement(5332..6201)
                     /gene="galU"
                     /locus_tag="MJE33_003148"
     CDS             complement(5332..6201)
                     /gene="galU"
                     /locus_tag="MJE33_003148"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBY9649067.1"
                     /translation="MTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIEEA
                     IESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHYIR
                     QKEPKGLGHAIYCAKSFIGDEPFAVLLGDDIVDSDVPCLKQLIDTYNEYKTTVLGVQK
                     IAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFSILEK
                     QTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENLKD
                     DLMDYMRKKVSEK"
     gene            complement(6399..6719)
                     /locus_tag="MJE33_003149"
     CDS             complement(6399..6719)
                     /locus_tag="MJE33_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649068.1"
                     /translation="MSSKYNVWTFNYEFLGLDSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSGLDSDGNSILGELISEMDIDEDVSVDMTYQELEVDLEDLDSYLDEDIDSDIKGI
                     LDEI"
     gene            complement(6709..8700)
                     /locus_tag="MJE33_003150"
     CDS             complement(6709..8700)
                     /locus_tag="MJE33_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649069.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNKDTGVKYIYSFSDNKEKLEAVLEDSQIIIYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKFYMGGINKDNEKFIFISGKNRFEIN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCEIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIILYDNKNVFNLHINDKNREIMQIDDLKDIENEIVGYTLKHAPFFIQEDFDSL
                     TILKSFQKEIISIKNSDIKDIVINKELENENSNFVETEIKFNNQKVLLNLSKSMVQKL
                     MQDVFIYAKQPLLKENSIEVIYKNWSKAMNDMIIFNFFGNIYYMKSEFDKILEKELND
                     EIRIEVINSLYKQIQEQRNNLDLLSAYMPRILENQEIDLFEKYNTKLDVQVFKQIKNL
                     LSDLSYNISSYLNEVEKSLDNIIFVISGEDKKKYNYRMLKESESASLDVFLKQAISRL
                     NHLVENMYPYYVDETSREMFKLFELLWKNYRNIDDDSIKEILFERITNTYVFKQLTLN
                     NSTKERRKDIIEKIYNSVDYGTNKLDENMFFTGGIKYVK"
     gene            complement(8823..11366)
                     /locus_tag="MJE33_003151"
     CDS             complement(8823..11366)
                     /locus_tag="MJE33_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HBY9649070.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKEGLDSLKANDLNSFLAYDKMELSERRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVPNNVEENNEQDKVEIKATEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKSNDIESKFIDKLNSLNNESVSQTIHNEYK
                     KLCGLEELHSVEGYGFGKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFVDYVIKNKTEISEDTWNKAQNLIKDNYSELENHEIASKRTRRNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDAEEGVKEEIDSNNQDIGDVVEDKDTTDKEYDSNKEDIIE
                     PENKKSKKKAKLFGFIKKDNEEVEQEEENLNDISPDIILDKPVENHQVKSEEIEQNEL
                     KEIKQEEPSQHIEEERNVKIEKPINNNLDEKVSSNNESKLEKESKNLEDKKAKEIKEE
                     KLENEKSVVIPIKKKENSNKKSKNSSKDKYRENKKEMKNYVSDKEDSLDDEEVVSKKS
                     RLKETIIAVVIVVIVGVGYFITVGNNKKNDKENIPKSSTQQQANNKLTEEEKKAQAEK
                     EKKEAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGVYYTVYAGSLKVEKTAKETAKE
                     YEAKGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKYYDYKLE
                     ELKESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            complement(11644..11978)
                     /gene="rnpB"
                     /locus_tag="MJE33_003152"
     ncRNA           complement(11644..11978)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="MJE33_003152"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            12110..12787
                     /gene="srtB"
                     /locus_tag="MJE33_003153"
     CDS             12110..12787
                     /gene="srtB"
                     /locus_tag="MJE33_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HBY9649071.1"
                     /translation="MKKLYRIVINIILVLVILYSGFNIYSKLTKYNHDTKISSELQKK
                     EYKKEDLSNINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDFYKKDSISGTLFMDYR
                     NKSIDDKNIIIYGHNMKNKTMFNNLNKFKDADFFKKNNKIKITLNGKEFLYDVFSAYI
                     VESDYDYLKTNFNNESDYQNYINDITSKSLYKSPIKVNSNDKIVTLSTCTYEFDDARM
                     VIHGRLI"
     gene            complement(12947..13021)
                     /locus_tag="MJE33_003154"
     tRNA            complement(12947..13021)
                     /locus_tag="MJE33_003154"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12987..12989),aa:Thr,seq:tgt)
BASE COUNT         4048 a         2299 c         1330 g         5426 t
ORIGIN      
        1 aattcagaca aaaaacgata aattttttta aaagctttta ttcttactct ataatcatat
       61 ataataaaaa tgtaaaaata tcttaaaggt taacttctac aaatatataa ataaaaatac
      121 ctatgaaagg atgaacaaaa attgaattta tatcacttac gctattttgt tacattggca
      181 catttagaac actatacaaa agcggcagaa aatttatcta taacacaacc aagcttaagt
      241 catgctattt ctttactaga aaatgaactt ggggtggctc tatttgaaaa agaaggtaga
      301 aatatagtcc ttactaagta tggaaaaata tttttaaaag atgtggaaaa atcattagaa
      361 attttagatt caagtgtaaa atcattaaaa ataactggta ctggtgaagg acaaatagac
      421 ttagcttttc ttagaacact tggtactgat tttatcccag acatagtgca caaattttta
      481 aaatctaatc ctgctaaatc aatagatttt aaatttcata ctggtgtaac tactgacatt
      541 attcaaggtc ttaaagaaag aaaatatgat attgcatttt gttcgaaact tgaaaaagaa
      601 aagggaattg aatttatacc tgttgccaaa caagatttag tactaattgt accatatagt
      661 catccactag ctgcaaagga tactatagat ttaaaagaaa ccatacctta tcctcaaata
      721 gtatttaatc agagaagcgg tcttagatat ataattgatg atatgtttaa aaaaataaat
      781 caacagccta atatagtcta tgaagtagag gaagaccaag taattgctgg attagttgca
      841 aaaaatttcg gaattgctgt agtcccaaat atgaatatgc ttagctttac aaaagtaaaa
      901 gtaattcaga ttattcaccc aagttgggaa agaaactttt acttagcatt tataaaagat
      961 agatatttgc ctcctgcaat taaaaacttt aaaaactttg tcattaaaaa cgctcaatta
     1021 taatttatta ataaaggggt tatttttaaa tatcctcttt tattcttttt ataaaataaa
     1081 aaaatctata cttttaaaag tatagattcc taactgacag tcggcatccg agtctcagta
     1141 attatcgtta tacaacagca actggattca gttcctgaat aactaggtta cttacattat
     1201 atcagatttt ttaaaatttg tcaattgtat ctaacgcatt ttttcaagcc atggtccaac
     1261 aggctctaca ataggaactt ctttaaatcc ccaatttctt atatcattca cactcgttgg
     1321 agtatatatt ttgtttctga cagatttgaa tattttgttt ttttctccat attgcataca
     1381 ctttggatta gcttctatta accctcttct tgctctgcta agaggattac ataaaatatc
     1441 tgctatttct aaaccttctc tatagtcagc atcttctttt ttggcaatta taaaaccctt
     1501 aactttatct cttaattctt catctatatc taagtatatt gttccattat taaatacatc
     1561 aaaaaacgcc ttttgtactt ttaagtttga gacatcatcc cttgactcta taactattcg
     1621 tgcagattct acagtttcat tatttacaaa tgaataaaaa ttctgaagaa catgtataaa
     1681 agccacaaca tacggttcct ttgaaggtgc ataatatttc tttaatctag ctttatctac
     1741 tgttactgat attatagtac actctagtct agatagaaat aaaggcaact catcatagaa
     1801 taacttaatc tgtgaatcat caaacttctc aaatgctctt gattttcttg atatatctgt
     1861 caaatgtatt cctatgttac tagttccaaa acaatactct ttaaatgcat ccatttcttt
     1921 cctcaaagaa catacataat cgccttgact tactgcatat ttatattcaa aaattactcc
     1981 tgtcaatgta aatgggtctt catgatttat atcagctctt ccactttcat caataaacat
     2041 tatatatttt ttctttataa tcttactcac cctcttaaca tatgatgata gcatctatat
     2101 tcattttata tgagttttaa aaatattcca agatttttat ataacttaaa cttaatttta
     2161 tactaaaaaa ttgagatgta aaacaatcaa gcttttattg attgaatttc acatctcaat
     2221 tataattatt tttcaatata tatttttact attttgcaag aatatccatt aatttatcca
     2281 ttactatata agatactccg cctcctattt gataaggagt ttcaactttt ttatttgcaa
     2341 atactctttg ttgctctgat gataatcctg aattatccat taaaactatt ggtgaatgta
     2401 attttacagc tagtggtcca gctgataaag catctactaa agcatctgat ttactaacta
     2461 atacagcctg taaatcagta tctccataga atttttctat tactttggca ttagtatcta
     2521 ctctactatc tccatatatt ctatttcctg aaatatcttc agtagttata tcattcattt
     2581 ttgctattac tgaatcgtta attgcacttg gtccacctat aaagtatgca ttttctaatt
     2641 ttctttctgt tagccattta taagattctt ctgtaattcc atctttattg actaatatta
     2701 taggttgttt ttcttcccca gctttagaag ctacagataa agcatctgcc tccccatttc
     2761 cagaaaccat atatgcagtt tttatttctt ctcgatttcc aagttcttca gctattcttg
     2821 tagcagtttg atatctatct tgacctgcta ttctctttgt ttctgtgttg aaagtagttt
     2881 ttatttcttg ctctaattta gaacttattg cattttctcc acctatcaag attactgttt
     2941 taggtgctaa taacttcatt tcttcaacag tttcagttgg taaattagct gtattagtaa
     3001 gtagtattgt tgagtcatag ctacttgcta aaggagttgc tgttatacca tcagctattg
     3061 cattaccatt tactaatatt acagtatctg agctatcagc attccagttt ccaactgaac
     3121 ttatcttttt agctgtatca aatctatttg cacctataat tgtatcccat gtaggattta
     3181 tagttccatc ttcattaaaa gtataatctt tcccatctat tttttgagaa ccagttagca
     3241 tctttccatc tttgtctaaa tagtaagact taccatctat ttgttgccat cctgtctgca
     3301 ttattcctgt tttgtcaaag taatacttat taccatctat ttcttgccat cctgttactg
     3361 ctaaaccatc agatcctaga tagtatttct ttccattctt ttcttgccat cctgtttgca
     3421 ttactccatc tgagttaaag taataagttt ttccgtctat tactttccat ccagatactc
     3481 ttgctccatc agttcctaaa taatattttt tgttattaat tgttttccat cctgtttgca
     3541 ttactcctga agcatcaaag taatacattt ttccatttac ttttagccaa ctattctttt
     3601 ggtatgttcc atcagatttt acataatact tcttaccatt ttctgttttc catcctgttt
     3661 gatttagatt tatcttatat tttgccgcta tctttacata gctgtcatta ctataatcta
     3721 taattacatc tgaacctaca ggaacttggt caaataacca tctaacatct ttattgtgca
     3781 ttcttataca tccaccagaa atatgctttc ctatagaact ctcattatta tttccatgta
     3841 ttccatatgt atctccataa gttcctttta atgctaatcc catccatcta tctcctaatg
     3901 gatttcttgg acttcctcca ggtatattac ctttataata tggtctattt tttatcttgt
     3961 taactacttt tgttttccct gttggtgttt ctgacccctt tttacctgtt gcaactctaa
     4021 attcttttac aagtttatta tttacaaaat atcctagagt attttttctt gaatttacta
     4081 taatcacatg tttactggct gcgcttgatt ctggtacgcc acatgcaaat actaaaacta
     4141 aagtcagtgc tagtactaaa tactttttta gacttttttt cactataact tccccctctt
     4201 ttagaaacat tactacatgt gtctaattat aacataaaat taccaatacc ttatatttac
     4261 aaatttgtta catttataca tataagtata tattctattc ttcataccca ttaggattca
     4321 tactttgcca tctccatgaa tcttcacaca tctcttcaag ttcatactta gctttccatc
     4381 caagctcttt ttctgccttt gaagaatctg cataacacat ggctacatct ccagcccttc
     4441 ttcctacaat cttatatggt atttcttttc cacttgcttt agaaaatgct tttactaaat
     4501 caagaacact ataccccttt ccagttccta aattataaac aacaagtcct ggattttctt
     4561 ctaatttttg taatgctttt acatgaccag cagctaaatc taatacatgt atatagtctc
     4621 taactccagt tccatcatgt gttggatagt cattaccata aacactcaat tccttcaatt
     4681 tacctactgc tatttttgtt atataaggca ttagattgct cggaactcca tttggttctt
     4741 cacctattct accactttta tgagcaccga ctggattaaa gtatcttaaa agagctatat
     4801 ccaatgactt atctgctttt gatatatcta ctaacatctg ctctatcata agtttagttc
     4861 ttccatatgg attggttaca gaaagtggaa aatcctctaa tataggacaa gtatgtgggt
     4921 ctccatatac agtagctgaa gaactaaata caaacttttt aacaccatat tctctcatta
     4981 attcaaataa atttaaggta cttattagat tattgctgta atattcaatt ggtttttcta
     5041 ctgattcacc aactgctttt aaagctgcaa agtgtataat tgattcaata ttattttctt
     5101 taaatacaac atgcatttca tctttatttc tgatatcaat attataaaat tttactggtt
     5161 ttttagataa ttccttgatt ctatcaacca ctattgagtt gctattactt aagttatcaa
     5221 ctataacaac ttcataacct gattctaaaa gttctattgc tgtatggctt cctatatatc
     5281 ctgctcctcc agctactaaa actgccattg taattacctc ttttctaata tttatttttc
     5341 acttactttt tttctcatat agtccattaa atcatctttt aaattttctc tctttagagc
     5401 aaagtctatt gttgcctcta aaaaaccaat cttatctccc acatcatatc ttctaccttc
     5461 aaagttataa gcataaatag cctcttgttt tccaagagtt tgaagtgcat ctgtcagttg
     5521 tatctctcca cctttaccag gtgtctgttt ctctaatatg ctaaatattg caggagttat
     5581 tatatatctt ccaagtatag ctatatcaga tggagcttct tcaattgcag gtttttcaac
     5641 catatcttta actttatata ctctatcttc gatatgtttt acatccaata taccatattt
     5701 attagtatct tctttagcta tcttttgaac acctaataca gttgttttat actcattata
     5761 agtatctatt aattgtttta agcacggaac atctgaatct actatatcat cacctaataa
     5821 tacggcaaaa ggttcatcac ctataaaact ttttgcacag taaattgcat gtccaagacc
     5881 ttttggctct ttttgtctta tataatgaat atttaccata tttgatatgt ctcttaccat
     5941 ttctaacatc tctgtcttac ctttttgctc tagttctaac tctaattcaa ctgatttatc
     6001 aaaatgatct tctatagatt ttttatttct acctgttatt ataagaattt cttcaattcc
     6061 cgattctata gcttcttcta ttatatactg taaagttggt ttatctacta taggaagcat
     6121 ttctttgggc tgtgattttg ttgctggtaa aaatctagtt cctagccctg ctgctggtat
     6181 tattgctttt ttaactgtca tctgcatttt ttcacccttc ttcgtaaatt ttctattgta
     6241 ccaaattaat ataccatatt tatacttttt cttcaaactc tatattatcc caatatttaa
     6301 tgctataaga taatataagg tagatgaaat ttcatctacc tttagtttta taagttaata
     6361 ttgtattgtt tatattttat ccaaaactta tacaggtact aaatttcatc taagatgcct
     6421 tttatatcac tatctatatc ttcatctaga taagaatcta aatcttctaa gtcaacttct
     6481 agttcttgat atgtcatatc aacactgaca tcctcatcta tatccatttc tgatattaat
     6541 tctcctaaaa tactatttcc atcagaatct aaaccactat ctatatcttc atctatatag
     6601 ttttcattta aaatttcatc taaatcaatc tcgtaataaa cttcattatt tttttcatcc
     6661 cctgagtcca atcctaagaa ctcatagtta aaagtccaaa cattgtattt acttgacata
     6721 ctttattcct cctgtgaaaa acatattttc atctagttta ttggttccat aatcaacaga
     6781 gttatatatt ttttcaatta tgtcttttct tctttctttt gtactattat ttaatgtcaa
     6841 ttgtttaaat acatatgtat ttgttattct ctcgaataat atttctttta tactatcatc
     6901 atcaatattt ctatagtttt tccacaataa ctcaaataac ttaaacattt ccctactagt
     6961 ttcatctaca taataaggat acatattttc tactaaatgg tttaatcttg atatggcttg
     7021 ttttaaaaat acatcaagac ttgcactttc actttctttt aacattctat aattgtattt
     7081 cttcttatct tctcctgaaa ttacaaagat gatattgtct aaactttttt caacttcatt
     7141 taaataactg cttatattat atgataaatc agacaataga tttttaatct gcttaaacac
     7201 ttgtacatca agtttagtat tatacttttc aaacaaatct atttcttgat tttctaatat
     7261 tcttggcata tatgctgaca ataaatctaa attgtttctt tgttcttgta tttgcttata
     7321 aagtgaattt ataacttcta ttcttatttc atcatttaac tctttttcta aaatcttatc
     7381 aaattcagat ttcatatagt aaatatttcc aaagaaatta aatattatca tatcattcat
     7441 agctttagac caattcttat atatcacttc aatagaattt tctttaagta gaggttgctt
     7501 tgcatatata aatacatctt gcataagttt ttgaaccata cttttactta agtttaaaag
     7561 tactttttga ttattaaatt ttatttccgt ttctacaaaa ttagaatttt cgttttcaag
     7621 ttctttatta attacaatgt cttttatatc actatttttt atagatatga tttctttttg
     7681 aaaagacttt agaatagtca agctatcgaa atcttcttga ataaaaaatg gagcatgttt
     7741 taatgtatat cccactattt cattttctat atcttttagg tcatctattt gcataatttc
     7801 tctattttta tcatttatat gtaggttaaa tacattttta ttatcatata atattatatc
     7861 ttctcctaaa tttaacttag aacaattatc tttcgctgac tttacaatag tcttcttaga
     7921 tttcttattt ataaaaatta attggtcttg atattttact aaaagcatgt ttgtgttttt
     7981 ataatcaaat ccatctatat ttcctgatat cttgcagaat actatttcac aatctttagg
     8041 aagatttcct attttttggt tatccttaac tctctcattt aaatcagatg ggactacact
     8101 atctatattg ttattaaata tagatacaga ccttactata atcccctctc tttccatatg
     8161 aaagtatccc ttcaatgaca ctcttttatc ttctaaaatg tatctttcta tatcatcaaa
     8221 atttatttca aatctatttt tcccactgat aaatataaac ttctcattat ctttattaat
     8281 tccacccata taaaacttat tgtcatctaa acttaatata gtcatattat cctttagata
     8341 gaatataaat tctctttgac ttttgcagat taaatcatat tcatccaaag ttattttcat
     8401 agtcaaattt gctgtaacat ttttacttga attttcactt tttatgtaaa aaggcaagtt
     8461 atttccatag ataattattt gagaatcttc taaaactgcc tctaattttt ctttattgtc
     8521 tgaaaaacta tatatatatt ttacacctgt atccttatta acttcatctg atttgctgat
     8581 tattatattc cctagatttg ttatatgaag ctttttatat tcttttgagt atctacttat
     8641 gccactaagc tcaaacccat ctgaaagctc atatatgatt ttttctccta atttcaacat
     8701 accttcacca cctaaacaga attataagta ttttataaag gtgaacaaaa tgtctcacaa
     8761 taaataatat tttttttaat actttttatt ctgagacata gtttctcctt ttctataatg
     8821 ctctaagcac cttcaactat ttcttcataa agtttatcta aatgtttaac atattcacga
     8881 tatccagatt tgttctttaa ctcacttctt aaatcttcat atttttgttt aagttcttca
     8941 gcactcaaag aaggagctga ttcttttaat tcttctaact tataatcata gtacttatca
     9001 tacatagcta tatatgtatc aatagatttc ttagctagtt cattacattt ttcttgtgct
     9061 tctccatatt gagaataatc acctattttt attttatagt atccattttc ttgaattatt
     9121 gttgaagaaa tacctttagc ctcatattct tttgctgttt cttttgctgt tttttcaact
     9181 ttaagtgaac ctgcataaac agtgtaatat acacctttac catctttata tgcttccatt
     9241 tcttttgctt tcttttcagc ttgtgctttt tcttgagcct ctttttctgc ttttgccttt
     9301 tcttctgctt cttttttctc tttttcagct tgtgcttttt tctcttcttc agttaattta
     9361 ttgttagctt gttgttgagt tgatgacttt ggaatattct ctttatcatt tttcttattg
     9421 ttgcctactg ttataaaata tcctacacca acaataacca ctataactac tgctataata
     9481 gtttctttta atctactctt cttagaaaca acctcttcat catctaaaga atcttcttta
     9541 tctgatacat aatttttcat ttctttcttg ttttctctat atttgtcctt agaagaattt
     9601 ttagattttt tattactatt ttctttcttt ttaataggta ttactactga tttttcattt
     9661 tctaattttt cttcttttat ttcttttgct tttttatctt ctaagttttt actttctttt
     9721 tcaagcttcg attcattatt tgaagatact ttttcatcta agttattatt tattggtttt
     9781 tctattttta cattacgttc ttcttctata tgttgacttg gttcttcttg ttttatttct
     9841 tttaattcat tttgctctat ttcttctgat tttacttgat gattttcaac tggtttatct
     9901 aaaattatat caggagaaat atcatttaaa ttctcttctt cttgttcaac ttcttcattg
     9961 tcttttttaa taaatccaaa taatttagcc ttctttttac tctttttatt ttctggttca
    10021 attatatctt ctttattgct gtcgtactct ttatctgtag tatctttatc ttctacaacg
    10081 tcacctatat cttgattatt actatctatt tcttctttaa ccccttcttc agcatcatct
    10141 tctattgaaa tactacttct aaagcaaata tctttatctg cattttttat catgtaaatg
    10201 tattcttcaa tatcgatatc tttatttctt ctagttcttt ttgatgcaat ttcatgattt
    10261 tctaattcac tatagttatc ttttattaga ttctgtgctt tattccatgt atcttcacta
    10321 atttctgttt tatttttaat tacatagtcc acaaattcct taaaattttc aggaccaaat
    10381 tctccaatta tagtcagtct cattgcacct tctataaggt ctctatttct tctctttaaa
    10441 aattctaata caaccgttgc acaagcaaaa tctttaataa tttctttgcc aaaaccatat
    10501 ccttcaacag aatgaagttc ttctaatcca cataacttct tatattcatt gtgtattgtt
    10561 tgagatacac tttcattatt taagctattt aacttatcta taaacttcga ttctatatca
    10621 tttgattttg attcatgttt tacttttgaa tttttaattt catttaatag catgtcattt
    10681 tctgaattat ctttacctaa ttgcaaatcc tcaatttcag ttgcttttat ttctactttg
    10741 tcttgttcgt tattctcttc cacattatta ggtaccacat ctttattgct agctaatatt
    10801 ttgtttacac agaatataat ttctcttttt aattctcttt tcgcatctgc ttgattttta
    10861 agaaataaat ctccacattc tttcttttct aaacttattt ttctaagaga tatattaaga
    10921 agatctttat ataggtttaa aaccttataa tccttttcta aacgtcttct tctctctgat
    10981 agttccattt tatcataagc taaaaaacta tttaaatcat tggcttttag agagtctaat
    11041 ccctctttca tagccttatt tacatacttt ctacattttg ctgtagttaa ttctaaatct
    11101 ttttctaatt catctatttc aggtatgtac ttatctacta tccatttgcc aataagtggt
    11161 tctgtatttc ttgctaatgt cttataaaac aatatattaa attttaaaag ctctgcatat
    11221 tttgcttcat taaattctac tttttcaaat ttcttttcca aaacaccatc tagcttttca
    11281 tttattttag actcttgttt taaaaaactt gctgtgacat ttaaaacagt gtttcttatt
    11341 atattatttt cactcaaaac cttcactcct taaatcaatt attttattgt aatatttaca
    11401 ttgtgcaaag ttgctaattt gattatgata tctaaataat tatttactaa atttgctaca
    11461 tcttatatca tatcatatta tttacatcat tttacttttt ctttttatat ctatcttata
    11521 ttataccatt tttaatatct gtactaactt atattacaat attcctcaaa attttaaaag
    11581 tagattagac aaatttacaa ataaaaaaac atcagtttaa actgatgttt ttgactatcg
    11641 aaataattaa gcaagtctat aagcggagtt ctgtattaga taatcatctt tctaggccta
    11701 ttgtcaccaa taggctcaag cgacctacct accggagagt acgagcagca ctctttattc
    11761 ctatcttggt cttgctccag gtggggttta catagccaac tagtcgccta gctgctggtg
    11821 agctcttacc tcgcctttcc atccttacca caatagtggc ggtttatttc tgttgcacta
    11881 tccttaggat cgctcccact aggcgttacc tagcaccctg ctctatggag ctccgacttt
    11941 cctcatgtaa gattacatgc gattatctgg cttacttaat atttatttta cttagttatg
    12001 ttaacacaaa ttcttttaca tgtcaaagta atattatact aaaaatttaa atttattcaa
    12061 aaatagtgta atatagatat ataaactaca tttaactcga ggtgatatat tgaaaaaatt
    12121 atatagaata gtaattaaca ttatactagt attagtaata ctttacagtg gatttaatat
    12181 atactcaaaa cttaccaaat acaatcatga tactaaaata tcctcagaac ttcaaaaaaa
    12241 agaatataaa aaagaggatt tatcaaatat taacagtgat tttaaatttt ggctttctgt
    12301 tgagaataca aatataaatt atccagttgt acaatctaaa gataattctt actatcttga
    12361 caaagatttt tataaaaaag attctatttc tggtacactg tttatggatt atagaaataa
    12421 atctattgat gacaaaaata tcataatata tggacataat atgaaaaata aaactatgtt
    12481 caacaaccta aataagttta aagatgctga cttctttaaa aaaaataata aaataaaaat
    12541 aaccttgaat ggaaaagaat ttttatatga tgttttttct gcttatattg tagaatctga
    12601 ttatgattat ctaaaaacaa attttaacaa tgaatctgat tatcaaaatt acataaatga
    12661 tattacttct aaatcattgt ataaatctcc tattaaggta aatagcaatg ataaaatagt
    12721 tacactttct acatgtacct atgaatttga tgatgccaga atggtgattc atggtagatt
    12781 gatttaaaac tcacagacac agtttaaaac aataatttta aactgtgtca tcttttatac
    12841 ctataatttg taaattagac cactaaaagt atttaataca aaataacaaa tacatcaaac
    12901 aataaaaaac aataaaaaaa gactatgcta aaaacatagt ctttactgga gctggtgata
    12961 ggaatcgaac ctacaacctg ctgattacaa gtcagctgct ctaccgttga gccacaccag
    13021 cacacctatt ttattgtaaa ttagcaaaac taatttttaa aagttaacac tctaaaaact
    13081 acacatatat cttaatgaat gaa
//
LOCUS       DAGSRM010000108        13051 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.108, whole genome shotgun sequence.
ACCESSION   DAGSRM010000108 DAGSRM010000000
VERSION     DAGSRM010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13051
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.108"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(91..801)
                     /locus_tag="MJE33_003155"
     CDS             complement(91..801)
                     /locus_tag="MJE33_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY9649072.1"
                     /translation="MTSICICDDEKQQRNMLNKIITREMDLWGYPYQIKEYSNGESLV
                     YNLEHENYYIDIIFLDIELGKSNGVDIAKTIRKHSKDTIIIFVTGFSDYVFHGYDVGA
                     LNYILKPYKTEKICKVLKEALKKLEQWKENFITVQIGTNLCKINTKDILYFKSELRKL
                     TVVTFDKTLEYYGKLNDMETVLPSNFIRTHQRYIVNLNHVDSITQTYAKVQNEKIPIS
                     RKKYQEVVGKFTRYMLES"
     gene            complement(1185..3296)
                     /locus_tag="MJE33_003156"
     CDS             complement(1185..3296)
                     /locus_tag="MJE33_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9649073.1"
                     /translation="MTINKLYSKLRAYNWKNYTTLFFCIILSVVLVTSYALIYFSPTI
                     QNILPTGGDSRKQATMIFSVAILGCAVFTTYASSLFLKFKSKEIGIFMALGTDKKKLK
                     KLLFSEILLISIVCCFLGLILSIPVSFGIWKIFQLVIIDTREMTYHFGTQGFIYGFIF
                     SVFVTLCIFILSVKFINRTNIIDILNDQRISEPIQDVKDWYGILGIIFIILGIFLGYL
                     VPSLTINKLNYRLPSVWSFTYLLSLIGIYMIMTYIVVHAKRGKHPEKYYKNIISTSMM
                     RFMGRQTVKNMCVISFLIAGALFAVFYVPNMVTGIYDTLKNNPIDYTFTYNQRDKQVS
                     KKDIEQLADKHNIKIQNYMEIPSIELIVDGVESIYHKDGTIEKVYMKKSSYDMFFNSK
                     DFSKLIGKPITIKNGEYLAVVNKGNENQDYKYTKFISSPVTEKEIPFRYAGKIIFNGS
                     FFQNRSMNAYVLNDEDYKSFTKDLSIKNYNNSILFNVDKDTYAFANDLKKEIIKRTSK
                     EMGMFSGYDEYVKKRYESTGRKYFMDEEYPSGEGHLKLDPNNNQLYLNWKYYPNFKIL
                     QSQDMIKNMAVFLMLFIYVAIICFTAVGIIAYTRGVTIAINHKQVFMDLNRLGANNDY
                     IKFCIRNQLKKVFSYPLIAGSFLIYLFNFMIMLNNDGRIVNSEVNALLINLGFIGICG
                     VYMYLIYLLTYKKFKKIIGIL"
     gene            complement(3286..4038)
                     /locus_tag="MJE33_003157"
     CDS             complement(3286..4038)
                     /locus_tag="MJE33_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9649074.1"
                     /translation="MLKVNQLCKSYKTGSKTYPVLKNISFEINKGEFVAIMGSSGSGK
                     TTLLNCISCFIPIDNGEIILGNNNIRNLKEKELSEVRNQQLGFVFQDFMLFDGLSVFE
                     NICVPQIIKGSQVLQMENRAINLCELFGIEHIRKKYPAEISGGEKQRTAVARALMNNP
                     DLILADEPTGNLDSKSCRTVIESFLKAKQELDATILMVTHDSFAASFCDRVIVLKDGI
                     IYKEVVKSNSRHKFLDELLEVLKEMGGAQDDN"
     gene            complement(5146..5322)
                     /locus_tag="MJE33_003158"
     CDS             complement(5146..5322)
                     /locus_tag="MJE33_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649075.1"
                     /translation="MFFTFIGVISAILLLCTIICGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
     gene            complement(5426..6034)
                     /locus_tag="MJE33_003159"
     CDS             complement(5426..6034)
                     /locus_tag="MJE33_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY9649076.1"
                     /translation="MGKLLRGKIMKSDSTKEQLINATIQLLSSHRDVSSITAREIVAK
                     ANVNLAMINYYFKSKEELINISIKKFLKRHMDEYKATHDTKNLAPKQQLREILIAFFD
                     FIGEHSQFIKISVPYILIQEEIVYPFEILPLIKAHYENQNNKKTEAEYRTIAYQITSF
                     IQLLFLRSNAFFKYSGIDILNIEQRNGLIDFQLDLLLVDITK"
     gene            complement(6133..6957)
                     /locus_tag="MJE33_003160"
     CDS             complement(6133..6957)
                     /locus_tag="MJE33_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBY9649077.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAEIDKYTGHRLYSVE
                     QISILQRIVLLRDSKFSVAEIANIVHNWNDEFVIKELNRKKNEIQKEINQEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISLREIIPDYQSEGILWEKLSKFIKEEHIE
                     VSRQSNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHKGEHNEINPENYLTEIQIPLIKV"
     gene            complement(7135..8028)
                     /locus_tag="MJE33_003161"
     CDS             complement(7135..8028)
                     /locus_tag="MJE33_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBY9649078.1"
                     /translation="MQLYNTPFNTNKHLIYFFTFTLVLNWIFSLTPALLGITNTILGE
                     IIFYLAYTSPAIVGLFFISTIYPQSAKQDFIYRYLSFKEIRIKWILLTILFFALTFIT
                     SLIIGNYFNVKTSDIDWIHIAVFRPSKIAYMLLASFILSILSQEAGWRGYAIDKLLVR
                     FGFIGSSIILGLVCGIWYLGSYFTPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVY
                     INTSRSILSAGLVHMMYYFFNIQLLLHYPIKLCVIAQYVQIIFGLIFLVYMISSNKFK
                     QKVNSEIEQIKSDDFEFELDW"
     gene            complement(8285..8722)
                     /locus_tag="MJE33_003162"
     CDS             complement(8285..8722)
                     /locus_tag="MJE33_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HBY9649079.1"
                     /translation="MKIHVEQGINCDDIEITIKCSALDNNIEKIISLLSTPLLDIKGK
                     KSGEIYKLNVCDILYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSIKVISHNRMQATLKNNEKVIINRRYIKNIKEKLNI"
     gene            complement(8979..9128)
                     /locus_tag="MJE33_003163"
     CDS             complement(8979..9128)
                     /locus_tag="MJE33_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HBY9649080.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LIFKK"
     gene            complement(9141..9371)
                     /locus_tag="MJE33_003164"
     CDS             complement(9141..9371)
                     /locus_tag="MJE33_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HBY9649081.1"
                     /translation="MEIKKKLVMSFKNVKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLASVVEAKVVETNTTPYDLVIE"
     gene            complement(10189..10410)
                     /locus_tag="MJE33_003165"
     CDS             complement(10189..10410)
                     /locus_tag="MJE33_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HBY9649082.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVVKSKSFANVKAEALNED
                     VYAVAEAIASLQENPIVDILKLDSTSLSK"
     gene            10990..11247
                     /locus_tag="MJE33_003166"
     CDS             10990..11247
                     /locus_tag="MJE33_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBY9649083.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVAKLYGGTNTYIPTYKGL
                     FRYARNRDIVKQFNGVNHNELAIRYNMCVSNIKRILNENNV"
     gene            11716..12954
                     /locus_tag="MJE33_003167"
     CDS             11716..12954
                     /locus_tag="MJE33_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBY9649084.1"
                     /translation="MNKGFYKLNSVVLNFDIKNDVNKIRFNHYIMRKENIQRLQGTLP
                     NGQFYMTVRRAATDLQLSISTISRLVKEFLDLGIIRLVSKGVKGNRSVYSYVSIEFNG
                     SLFDDSKYMELDCVCNDEFIDNSGIEFDCDSKDNSFNGDINVETVHCINEYLSDDDVE
                     HNYNNFHNKDTIKDKAISNISTIKKYIDESFDKNKSENSNLQQESINFNFNDTCNEID
                     KRTNNSIENNQNKRPMRLSDISVLSDKGMTNKKNYFNHNHGIQKGTKSVTKKKESLNK
                     FNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEVKVAKWK
                     NTIMEMYIRPETLFSYKFESYVNENISMKSQSNVHPCKEGNSNDSSNYIHSDYTTYDG
                     SVRERKKYENVRYWNPICEI"
BASE COUNT         4806 a         1580 c         1714 g         4951 t
ORIGIN      
        1 aaaattaaat ttgaaaaaac aaaactctaa aaactaagct ttaaaaaatt aagtttcaaa
       61 aataaaattc tagagattga acttcaaaaa ttaagattct aacatatacc ttgtaaattt
      121 tcctacaacc tcttgatatt ttttacggct tataggaatt ttttcattct gaactttggc
      181 ataagtttga gttatactat ctacatggtt tagattgaca atataccttt gatgcgtacg
      241 tataaaatta gagggaagaa cagtttccat atcatttaat tttccgtagt attctaatgt
      301 tttatcaaag gtaactacag ttaattttct tagctcactt ttaaagtaga ggatatcttt
      361 tgtattaatt ttacaaagat tagttccaat ctgaactgta ataaagtttt ctttccattg
      421 ttctaatttt tttagtgctt cttttaatac tttacatatt ttttcagttt tataaggttt
      481 taagatgtag ttcaatgctc ctacatcata tccatgaaat acatagtcag aaaaacctgt
      541 gacaaagatg attatagtat cctttgaatg ttttcgtata gtttttgcta tgtcaacgcc
      601 attacttttt ccaagttcaa tatctaaaaa aataatatct atgtaataat tttcgtgttc
      661 tagattgtat actagacttt ctccatttga atattctttt atttggtagg ggtagcccca
      721 caaatccatt tctcttgtaa tgattttatt cagcatgttt ctttgttgtt tttcatcatc
      781 acaaatacaa atcgaagtca attttttatc tcctttcaaa ttttatattt atatagttaa
      841 gtatataata tattttgcta cctagatata taaaatgaaa taacaattat atccagatag
      901 caaaataaaa ttttttatat tagtatactt acacttaaaa ctattctatt aaacttattc
      961 tgaagtgcta atgattttct tattaaaaaa tctaacaatc tttcttatta taaaatacaa
     1021 atgcttttat tattataaaa agtaatgatt ttattacttt aaaaatctaa taacctttct
     1081 tattataaaa caccaatgat tttattactt taaaaatcta ataacctttt ttattataaa
     1141 acaccaatga ttttattact ttaaaaatct aataaccttt tttattataa aataccaata
     1201 atttttttga atttcttata agttaataga tatataaggt acatataaac cccacaaatc
     1261 cctataaaac ctaagtttat caaaagagca ttgacctcac tattgacgat tctaccatca
     1321 ttatttaaca taatcataaa attaaacaga taaattaaaa aacttcctgc aataagaggg
     1381 tatgaaaata ctttctttaa ttgatttcta atacagaact ttatataatc attgtttgct
     1441 cctaatcgat ttaaatccat aaagacctgc ttgtgattta tagctatagt aacacccctt
     1501 gtatatgcaa tgattcctac tgctgtaaaa catatgattg ccacatatat gaataacatt
     1561 aagaagactg ccatgttttt tatcatatct tggctttgta aaattttaaa gttaggatag
     1621 tatttccaat taagatacaa ctgattgttg tttgggtcta gttttaaatg accttcacca
     1681 cttggatatt cttcatccat aaaatatttt cttccagttg attcataacg tttttttacg
     1741 tattcatcat aaccactgaa catgcccatt tccttagatg ttctttttat aatttctttc
     1801 tttaagtcat tggcaaaagc ataggtatct ttatctacat taaatagtat agaattattg
     1861 taatttttta tggataaatc tttagtaaat gatttataat cctcatcatt aaggacatag
     1921 gcgttcatac ttctattttg aaaaaaagaa ccattaaata ttatttttcc agcatatcta
     1981 aaggggattt ctttttctgt gactgggctt gatataaatt tggtatattt ataatcttga
     2041 ttttcatttc ctttatttac aacagcaaga tattcaccat tttttatagt tattggtttg
     2101 ccaattagct tggaaaaatc tttagaatta aagaacatat cataggagct ttttttcata
     2161 tatacctttt cgatagttcc atctttatga tatattgatt ctacaccatc aacaattaat
     2221 tctatagaag gaatttccat atagttttga atttttatat tgtgtttatc tgctaattgt
     2281 tcaatatctt tttttgatac ctgtttatct ctttgattgt aggtaaaagt ataatctata
     2341 gggttatttt taagagtatc atagatacct gtaaccatat taggaacata gaatacagca
     2401 aataatgctc ctgcaatcaa aaatgaaata acacacatgt tttttactgt ttgtcttccc
     2461 ataaatctca tcatgctagt tgatatgatg tttttatagt atttctctgg atgttttcct
     2521 cttttagcat gtacaactat gtatgtcata atcatatata ttccaataag agagagtaga
     2581 taagtaaagc tccatacaga tggtagtcta taatttaatt tgtttatagt tagtgaaggt
     2641 acgagatatc ctagaaaaat accgagtata ataaaaatta tacctaatat accataccag
     2701 tcctttacat cctgtattgg ttcagatatt ctctggtcat ttaatatatc aattatattt
     2761 gtacgattaa taaattttac agaaagtata aatatgcata atgtcacaaa tacactaaat
     2821 ataaatccat atatgaatcc ttgagttcca aaatgatatg tcatttctct ggtatcaata
     2881 atcactaatt gaaatatttt ccatatacca aaagatacag gaatacttaa tataagacct
     2941 aaaaaacagc atactattga aattaacaat atctctgaaa atagaagttt ttttaatttc
     3001 ttcttatctg ttcctagagc catgaaaata ccaatttcct tgcttttaaa ttttaaaaag
     3061 agggaagaag cgtatgtagt aaagacagcg catcctaata tagcaactga gaatatcatt
     3121 gtagcctgtt ttctgctatc tccaccagtt ggaagtatgt tttgtattgt tgggctaaaa
     3181 taaattagag catatgaagt tactaatacc acagaaagta ttatacaaaa gaataaagtt
     3241 gtatagttct tccaattata ggctcgaagt ttactgtata atttattaat tgtcatcttg
     3301 agcgcctccc atttctttta aaacttctag taattcatct aggaatttat gtcttgaatt
     3361 tgattttaca acttctttat aaattattcc atctttaagc acaataactc ggtcacagaa
     3421 gcttgctgca aagctatcgt gtgtaaccat gagtatagta gcatctaatt cttgctttgc
     3481 ttttaaaaat gattcaatga ctgtacgaca agacttacta tcaaggttac cagttggttc
     3541 atctgctaat attaaatctg ggttgttcat gagtgctctt gctactgctg tacgctgttt
     3601 ttctccacca gatatttcgg cagggtattt ttttctgata tgttcaatac caaatagctc
     3661 acatagatta attgcacggt tttccatttg taagacttga ctacctttga taatttgtgg
     3721 aacacatata ttttcaaaga cactaagacc atcaaatagc atgaaatctt ggaatacaaa
     3781 tcctagttgt tggtttctaa cttctgaaag ttccttttct tttaaatttc ttatattatt
     3841 atttccaaga attatttccc cattatcaat tgggataaaa catgaaatac aatttaaaag
     3901 agtagttttt ccacttcctg atgaacccat gattgcgaca aattctcctt tgttgatttc
     3961 aaaagaaata ttttttaata caggatatgt tttactacct gttttatatg atttacataa
     4021 ttggtttact ttaagcatat atatcatcct cactttcatt tatcttgtaa gtcaatgata
     4081 gcatttgaaa ggggaggagg gatatgagct atattgtaat agttggatgt ttttttgtaa
     4141 tgtttgaaaa tatattgttt ggatagtgtg agaggttatt attatagtta aattgattat
     4201 attattggag gatttagagg gttattttta ggtattaatt gttgtggtaa agtaatatga
     4261 tgtggaacat tgaaggtgta gttagaaaaa aggtatatag taaaagtaaa aaggttatag
     4321 aataacgaat gttgtattga aaaaaatgat gtaaataatg tatagtaggg ttaaaaagaa
     4381 aaaaagaatt atagagcagt aagattattt aatggaatgt attgtaaata atttgaaagg
     4441 atttaggggt tattaacaca atgtaattga gaaaatatat tggagttttg gaaataaata
     4501 ggttgttaaa aacaattgaa atttataata aaaaggttga aaagtattat tagtataggt
     4561 atgatagata attttagaaa gtggaagtat tttagactat gctgaaaagt gattgatatt
     4621 attttgagat attaaaatat tactatatta tagtaaagaa gttaaaataa taaaatgttg
     4681 taataataaa ggtttgtagt ataataaaga actgtaataa ataagacttt gcaatgtaac
     4741 agaaatgctg taataataaa agtctgtagt ataataaaga actgtaataa ataagagatt
     4801 gtaataataa aagtctgtag tgtaataaag aactgtaata aataagactt tacaatataa
     4861 tagaaatgtt gcaataacaa aacaccataa taaataagag tttgcaatat aatagaaaca
     4921 ctgcaataaa taagagattg taatataaca aaatcctata ataaataaga ttttacaata
     4981 taataaaaat cctataataa ataggactct gtaatataat aaaatgctgt aacaacaaaa
     5041 cactataata aataagaccc tgtaatataa taaaaatact ataatatagt aaaaatcatg
     5101 caataataaa aatctataat aataaacctc agcataacaa agacattaat ttcttactaa
     5161 aaaggccacc acaattaaca gtgaaaaagc aattgaaagt attgaaaaag taaaatgaga
     5221 attagagtca ttattacgat tattaagttt tatccataaa ccacatataa ttgtacacaa
     5281 caataaaatt gctgatatta ctcctataaa tgtaaaaaac attaaaattc ctccccctta
     5341 tttaatgcaa gtatttttag tcaatgagaa tattttcaaa cgttaccata aaataaaaaa
     5401 atgtacataa aaaattaata tagtattatt ttgtaatatc taccaataat aaatctaatt
     5461 gaaaatctat taaaccattt ctctgttcaa tattaagaat atcaatacca ctatacttga
     5521 aaaaagcatt agaacgaaga aaaagcaact gaataaatga agttatttga tacgcaatag
     5581 ttctgtattc agcttcagtt tttttgttat tctgattttc atagtgagcc ttaattaaag
     5641 gtagtatctc aaatggataa acaatttctt cctgtatcaa tatatagggt acagaaatct
     5701 ttataaattg actatgttca cctataaaat caaaaaatgc aattagtatt tcccttaatt
     5761 gctgtttagg tgctaaattt ttagtatcat gtgtagcctt atattcatcc atatgacgct
     5821 ttaaaaattt tttaatagaa atattaatta actcctcttt agatttaaaa taataattaa
     5881 tcatagctaa attgacattt gctttagcta caatttctct agccgtaatg cttgaaacat
     5941 ctctatgtga agatagtaac tggattgtgg cattaattaa ttgttccttt gttgaatctg
     6001 atttcataat ttttcccctt aaaagtttcc ccataaaaaa attaaacatg tttaacacac
     6061 gtttagatac tatcatgaaa attttgggat gtcaatgggt gattagtact taagtattta
     6121 agtactaatc tattagactt ttataagggg gatttgaatt tctgtaagat aattttcagg
     6181 atttatttca ttatgttcac ctttatgtcc gatttgtcta tttatacctg atatttgata
     6241 atcactattt ttatctaacc aataacagaa cgattcatat gcaccagcta tattttcata
     6301 tggaccataa accatagtac atgccatcat atcaatttct tctgtctcac gatatataaa
     6361 tccagatttg ttctttccaa tcttctttac aaccattcca acttcaatat ctacaccatt
     6421 atctttgact tcttcatcat ggtaaaaagc tatgttattg ttggattgac gtgatacttc
     6481 tatatgttct tctttgataa atttagatag cttttcccag agtatacctt cagattggta
     6541 gtctggaatt atctctctaa gtgatatgat tttataactt ggaatctttt taaagacaac
     6601 attgtaatga atttcatctt tatcacagtt tatagcttct ataaatttat caattttatt
     6661 tattctttgt tgttcctgat ttatttcctt ttgtatttca ttcttttttc tattcaattc
     6721 tttaattaca aattcatcat tccaattatg tacgatattt gctatttctg caacgctaaa
     6781 ttttgaatct ctcaataata caattctttg tagtattgaa atctgttcaa ctgagtataa
     6841 tctatgacct gtatatttgt ctatttctgc aggttttaaa ataccaagtt catcataata
     6901 acgcaacata cgaatagaaa tttgtgttag ttttgaaacc tctcctattt taaacatgaa
     6961 aatatgacct ccttttcact tataatcagt atatattttg tttttatata cttcaagcgt
     7021 ttgtggaaat acaattattt tccagatgtt gcaataaatt atagagagta aattatattt
     7081 aagtagtaat cgtatcaaat catagattat cttaatttat aattcatatt aaaattacca
     7141 gtctaactca aattcaaaat catccgactt tatctgttca atttcagaat ttaccttctg
     7201 tttaaattta ttagaagaaa tcatatatac aagaaatatt aaaccaaata ttatttgtac
     7261 atattgagct attacacata atttaatagg gtagtgtaga agtaattgaa tgttgaaaaa
     7321 atagtacatc atatgaacta acccagctga taaaatgctt ctacttgtat tgatatatac
     7381 aaaattaatt ataaagttta aaagtacaat gtttggtata aataaaaaag catcaaatag
     7441 ggagtattgt gccaaattat atggtatttg attaggtgta aaatacgacc ctagatacca
     7501 gattccacaa acaagcccta aaataattga agagcctata aatccaaatc taactaataa
     7561 tttgtcaatg gcatatcctc tccatccagc ttcttgacta agtatactca atataaaact
     7621 tgctaatagc atataagcta ttttagaagg tctaaacact gctatgtgta tccaatctat
     7681 atcagaagtt tttacattaa agtaatttcc gattattaac gaagtaataa aagttaaagc
     7741 aaaaaataat attgttaata gtatccattt tattctaatt tctttaaagc ttaggtatct
     7801 ataaataaaa tcttgttttg cactttgtgg atagattgta gaaataaaaa atagacctac
     7861 aatagctggt gatgtatatg caagataaaa aatgatttct ccaagtattg tatttgtaat
     7921 tccgagcaac gcaggtgtaa gactgaatat ccagttcaat actagcgtaa atgtaaagaa
     7981 atagattaag tgtttgtttg tattaaaggg ggtgttatat agttgcattt tttcactatc
     8041 cttccgtttt ataatgaagt tttaattttt attaattgtt tatttatttt tatatgtatt
     8101 aagttatcgc ttgatgttag ttattgccta taataatatc ctatttttta tattttgatt
     8161 aattattttt acactaaata taattaatta gtatttaata ttgaatatat tatattatta
     8221 ttaattatta aataggtcaa taaatttgca attagtgata ttaataaatt actttagatt
     8281 tttattaaat gtttagcttt tctttaatat tttttatata acgacgattt ataataactt
     8341 tttcgttgtt ttttaaagta gcttgcatgc gattatgtga tattactttt atacttttta
     8401 tgtagtccat atttaaaatc atggatttag atattcgtga gaaggatgta ttagctaata
     8461 attttgataa tttatatatt tttaaatcaa tttcaaatac atcatcttca taataagcaa
     8521 atgttttatt tccaactgtc tcaaagtata aaatatcaca gacattgagt ttataaatct
     8581 ctcctgattt tttacctttt atatcaagta acggtgtact caataatgaa attatttttt
     8641 caatgttatt atctagagca gaacatttta tggttatttc aatatcatca caattaatac
     8701 cttgttcgac atgtattttc attaatcatc acctcatttt attactgtca ttagaatagc
     8761 attatatctt attgagtcca actagatgcg ttctaaattt caaattaagc atagtgaaat
     8821 tcaaaaataa aacagtgaaa ttcaaatttg aggtggtaaa attcaaaaaa atactatgga
     8881 tatttggata aattatccaa atatcctagt atttcacata gaatatgtct aattatttta
     8941 caaatagacc tattttatga cttacatata ttgtcaaatt acttcttaaa tatcaaaata
     9001 tttttggata atgtatcaat actgtttgat aaagtattta atttaccctc aattctaacc
     9061 aataagtaca tggatagagc tataggaaaa ccaacattgg ttattaacag ttgtaaacta
     9121 tcatccataa aattagcccc ctattctata actaagtcat aaggagtggt atttgtttcc
     9181 acaaccttgg cttcaacaac agaagctagg tcaaatcctc caaccgaaaa aatattttta
     9241 gatactatca aatccatgac agattttata ttttcttcag ttaaatcatc ctttggattt
     9301 tctatagaaa aagaaacttg tttatccttt acatttttaa agctcatgac taattttttc
     9361 ttaatttcca tataattacc tccgatataa taatttatat tttaattgtt tgcttgttaa
     9421 tattggtatt ttaattgtca atattaatgt tttgcttgtt agtattaata tttcaattgt
     9481 caatattaat gttttgcttg ttagtattaa tatttcaatt gtcaatatta gtgtcttgct
     9541 tgttagtatt aatatttcaa ttgtcaatat taatgttttg cttgttagta tcagtgtttc
     9601 aattgtcaat attagtgttt tgcttgttag tattaatatt tcaattgtca atattagtgt
     9661 cttgcttgtt agtattagta tttcaattgt caatattagt gttttgcttg ttagtattag
     9721 tatttcaatt gtcaatatta gtgttttgct tgttagtatt agtatttcaa ttgtcaatat
     9781 taatgttttg cttgttagta ttagtatttc aattgtcaat attagtgttt tgcttgttag
     9841 tattaatatt tcaattgtca atattagtgt tttgcttgtt agtatcagtg tttcaattgt
     9901 caatattagt attttaattg ttgactctag ttattgctga tatagaaaat atttaattta
     9961 tttcaattaa ttagattaaa gtagggtagc atctaaaata gctaacatgt ttaagttgaa
    10021 atacgtttaa tatattaatt tttcaaagca ttaatatatt aaagttataa tcaaaattga
    10081 agttataatc tgccaaatct atagcgtatt aagtgcgtta tataacttaa tcttttatat
    10141 aaagagttaa tttttttaat ttgtaaagca gagtacgttt aaaaggtact atttagataa
    10201 tgatgtacta tctaatttta aaatatctac aatagggttc tcttgaagac ttgctattgc
    10261 ttctgcaaca gcataaacat cttcatttaa tgcctctgct tttacatttg caaatgattt
    10321 acttttaaca actgccttac ccttatcatc aaaaccagca tccaatttta gttttatact
    10381 agataaattt tttgttgaaa caacttccat aaaattacct cctaaaaatt taatttcaaa
    10441 gatttgtcga ctttctttgt tagctataat actatcatct aagataatat cggagttaaa
    10501 cagtaatttc taaagatttt ttgtgtaaat ttttatagga aaaattttac ttttttgctg
    10561 atgaaaaagt ttacaagtta tttgtgaaga tatgttctta gtgttttgaa atagtatttg
    10621 tatatattca tgcttagttg agatagtttt gtatggattt tcatatgtgt agagttaaat
    10681 gttaggtact aaagtttatg ttaaattaaa tgttaaagct tatgttaaaa attttattaa
    10741 atttaatctg taaataaaaa agttaaaagt ccaatattaa cattttactt ttaaaccatt
    10801 aaaatcttta ttaaagctaa caaagttata agttatgaga aattttatta aagttaatac
    10861 aaaaattaat tttaaagcta agaaattttt tattagattc aataaaacta ataaatatag
    10921 tttagtcaat aatgtattaa ataaaaactt tgttaagtct ataaaaatat cttaaaggag
    10981 gtgtaactta tgaagataaa agtggaggat ataccagttc aatttcatgc aatggtagaa
    11041 ataattggaa ttgataagtt tatagaggtt gccaagttgt atggaggaac aaatacttat
    11101 atacctacgt ataaggggct ttttagatat gctagaaata gggatatagt aaaacaattt
    11161 aatggagtca accacaatga attggctatt agatataata tgtgtgtaag caatattaag
    11221 cgaatactca atgaaaataa tgtttaaata taataaaata atgtttaaac ataatgaaaa
    11281 tagccttgtt aaacgtaaga gtaaagacaa agaagatgtt ttataaagtt tttttagaca
    11341 aattaactaa atttactact tattaattta aatacattgt atattctata aaaaaatttg
    11401 ttaaatactg gagatatatt gagaatacaa agaaaatggt cgaaaatatt tgttggctaa
    11461 aaaatagata aaatgataca attgcttgta taaaggggaa aaataaacaa ataaactata
    11521 taaataaaga aatagcaatt aatatgacat aatatattta taaaattaag aatataaata
    11581 gatattttaa ggtattagtt gttaaattag gaaatattaa aaaaaatata tatttatatt
    11641 taaactattt tgttatttat ataaaaatac ttatctagtt actagcaatt attagtagaa
    11701 atgggggaaa tagagatgaa taagggattt tacaaattaa attcagtggt attgaacttt
    11761 gatattaaga atgatgttaa caagataaga tttaatcatt atataatgag aaaagaaaac
    11821 atacaaagac ttcaaggaac tctgccaaac ggacaatttt atatgacagt gagaagagct
    11881 gctacagatt tacaactttc tatttctact ataagtaggc ttgtaaaaga gtttttggat
    11941 ttagggataa taagattagt gtctaagggt gtaaaaggta atcgttctgt atatagttac
    12001 gtaagtatag agtttaatgg aagtttattt gatgatagta aatatatgga acttgactgt
    12061 gtatgtaatg atgaatttat tgataatagt ggcatagagt ttgattgtga tagcaaagat
    12121 aattctttta atggagatat aaatgtagaa actgtgcatt gcattaatga gtatttgtct
    12181 gatgatgatg tagagcataa ttataacaat ttccataata aagatactat aaaggataaa
    12241 gccatttcta atattagcac tatcaaaaaa tatattgatg aatcttttga taaaaataaa
    12301 tcagaaaatt ctaacttaca acaggagtca ataaatttca actttaatga tacatgcaat
    12361 gaaatagata aaagaacgaa taatagtata gaaaataatc aaaataaaag gcctatgaga
    12421 ttgagtgaca tcagtgtttt aagtgataaa ggtatgacaa ataaaaagaa ttactttaat
    12481 cataatcatg gtatacaaaa agggactaaa tctgtaacaa agaaaaaaga atccttaaat
    12541 aaatttaata aaaaaaatat acagcaaagc attataaata tgctaaataa aaaatcaggt
    12601 aagaatttta aaatcgattc tccaataagc ataaaactta tcaatgaaag gctaaaggaa
    12661 ggctatgtct tagaagattt ctacaaagtt attgaggtaa aagtggctaa gtggaaaaat
    12721 accataatgg agatgtatat aagaccagaa accttgttta gctataagtt tgaatcctat
    12781 gtaaatgaaa acatatctat gaaaagtcaa agtaatgttc acccatgtaa agaaggcaat
    12841 agtaatgata gcagtaatta tatacattct gattatacaa catatgatgg cagtgtgcgt
    12901 gagagaaaaa aatatgagaa tgtaagatac tggaatccta tatgtgagat ttaattagtt
    12961 taagaatgta tttatgataa ataagatatt aatagagaat agtgagctat agcaaatgat
    13021 atatcaatag ttggtagtaa gttatattaa g
//
LOCUS       DAGSRM010000109        12400 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.109, whole genome shotgun sequence.
ACCESSION   DAGSRM010000109 DAGSRM010000000
VERSION     DAGSRM010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12400
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.109"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            194..541
                     /locus_tag="MJE33_003168"
     CDS             194..541
                     /locus_tag="MJE33_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBY9649085.1"
                     /translation="MHENKKNLLDGSLAQYNDTAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGEHECDVKNGDTVFVPTGEWHEITNGSNEEMVAV
                     QITKINAGAEYRG"
     gene            complement(691..1191)
                     /locus_tag="MJE33_003169"
                     /pseudo
     CDS             complement(691..1191)
                     /locus_tag="MJE33_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(1218..2723)
                     /locus_tag="MJE33_003170"
     CDS             complement(1218..2723)
                     /locus_tag="MJE33_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBY9649086.1"
                     /translation="MNVAIYLRKSRADEEAEHRGEFETLSRHKTTLLKLAREQNLNVI
                     EIKEELVSGESIEYRPKMLELLGEIKNNNYDAVLVMDIDRLGRGNMQDQGLILDTFKK
                     SKTKIITPRKTYDLNDEFDEEYSEFEAFMARKELKLISRRMQRGKIKSIEEGKFIAQA
                     PPYGYDIKYLNNKERVLVINEDKATVVKIIFDMYLKGDGACKISNYLNELGYKTTTGR
                     TWYEKGIRDIIKNKVYCGYIVWNKVERKRNSSRTRGIEEQIEVKGKHEPIISEDIWNK
                     AQNIRKKRINSPTPNNKITNPLAGIVKCGYCNHTLIAKSNTTSNGYEKYMVCTNCKNK
                     ATKLYILENKILEVLNKWLVNYRLSLKDEKDNSKPENTLEHTLTNLNKELDTLEKQKE
                     RLHDFLEQGIYDNNTFGERLKILLSRIENVREAINKTNIDIEMQKERNTVKTQIVPQL
                     EKILSEYTEEKEPYLKNILLKSVINYAVYKKEPNQKSDDFSLILYPNIPRD"
     gene            complement(2922..3776)
                     /locus_tag="MJE33_003171"
     CDS             complement(2922..3776)
                     /locus_tag="MJE33_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBY9649087.1"
                     /translation="MEWIERLNKAINYIEENITKEIEYEQVAKIACCSTYHFQRMFAY
                     MADVPLSEYIRRRRMSLAAVELQNDDKKIIDVALKYGYSSPTAFNRAFKSIHGVAPSV
                     IKKREITTLKAFPPISFKISIKGAEEMNYRIEKKEAFRIVGVSQPLHNELEKNFEIVP
                     QMWQKVALDGTLQKLIPMMDSQLQGVLGISICNNSEEWKYFISVSSTKSIDNTLEEYT
                     VPAFTWAIFSGEGQCPQAIQELEKRIVTEWLPTSGYEYDNGPDIELYLNPDPQNAKFE
                     VWIPVVKK"
     gene            4161..4442
                     /locus_tag="MJE33_003172"
     CDS             4161..4442
                     /locus_tag="MJE33_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649088.1"
                     /translation="MPNIEKDLDGTTTITYLNDDIGVPTDENGYKISMTILHGKTICH
                     VVSPETILGRKFTLEEKQEKDTIIYGAIEKIMVDRQLQIKNSNKEVASH"
     gene            4592..5440
                     /locus_tag="MJE33_003173"
     CDS             4592..5440
                     /locus_tag="MJE33_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HBY9649089.1"
                     /translation="MIKSFLMLGQSNMAGRGFISEVTPIYNERIQMLRNGRWQMMTEP
                     INYDRPVSGVSLAASFADAWCCENQEDRIGLIPCAEGGSSLDEWNIDGILFKHAISEA
                     KFAIQSSELTGILWHQGENDSNNGNYKFYYKKLLSIIETLRKELNIPDIPIIIGGLGD
                     FLGKVGFGKSCTEYVFINQELQKFAFEQDNCYFVTATGLTSNPDGIHIDAISQRKFGL
                     RYFEAFHKKKHIMEALANESELIIPSNSKTYTKTEKIYINSMDLALGKISYDEFESKL
                     IKLNDN"
     gene            5492..6013
                     /locus_tag="MJE33_003174"
     CDS             5492..6013
                     /locus_tag="MJE33_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY9649090.1"
                     /translation="MIPLLILGLLKQNPNSYGYELLALMEKKHYKYIVNFTKGSFYYN
                     LQQLEEKKFIEKVENKQSNKNRETHNYIITELGCQEFEKLMYKYGSKTEYINLSFYAT
                     LLFANDYKNEKLIELLETQIKQTQVRISLLNESIENDKTNSHYFNRMLENSRSHHLVN
                     IEWFEKLLNDIRD"
     gene            6364..6897
                     /locus_tag="MJE33_003175"
     CDS             6364..6897
                     /locus_tag="MJE33_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HBY9649091.1"
                     /translation="MTEVIIVNDARIISQIKAGKKELFDTLIKKYYDSIYCYCYRHVD
                     NKMTAQDLTQDVFVTVINNIENYKHYGKFENYLYVIAKNKCKDFWKKKQPIYLESLDK
                     EISNQELSDIENKMFIKEALTKLPELEFEVIILRYYHDLKFKDIAKIMESSVSVTKYR
                     MSRALKKLELYLEGGKL"
     gene            6894..7655
                     /locus_tag="MJE33_003176"
     CDS             6894..7655
                     /locus_tag="MJE33_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649092.1"
                     /translation="MNNKEIKRSLQKYKVPKYDSSALEKTIKTIHELTLSNTYKRMNN
                     KDFFISQIGFIRKRTWIMQFIILCTICIFLFSTSADSNSFYNTLSLISIASPLFVLTN
                     IEELTKVYNSSILEIEFSTKNTLHKIIATRMLIFGISDLLFMIIILVSAKQIIDVNIL
                     YIIIYILVPFNLMCIGCMEIINRNKGKHTNYYCMIYGISLVLLIVTLSNFRDNLYQFE
                     AINQWLIVFLVTAVTLIYEIRKTWRHLCSFENIII"
     gene            7693..8559
                     /locus_tag="MJE33_003177"
     CDS             7693..8559
                     /locus_tag="MJE33_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9649093.1"
                     /translation="MELSVERLTKQYNNKIIVDKIDFKLNEGIYGFLGENGAGKTTLM
                     RMICGILSPTSGEVKVDGKNNLDMGEDFRDMIGYLPQNFGYYPDFTAKEFMLYIASLK
                     GLMPSYAKRKTQELLEMVGLSEVINKKIRTFSGGMKQRLGFAQTLLNDPKILILDEPT
                     AGLDPKERVKFRNLISEFSKEKIVILSTHIVSDVEYIADKILIMKKGRFVLTGSVEEV
                     TSKIKDKVWKCVVNENEIDYMSDRYCLANIHHSGGVANLRIVSDIKPHDNACNLKPTL
                     EDLYLYCFNEVL"
     gene            8580..9821
                     /locus_tag="MJE33_003178"
     CDS             8580..9821
                     /locus_tag="MJE33_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBY9649094.1"
                     /translation="MSTLIKFELKKIIKRKSTIAVILGSLFLSIILFALMPIQEKAND
                     ESGKEYRGLQAIELKKQQSKLNEGYITDKKVKNSIRDYQKMFNNPNNIVTNESGFSDF
                     KPDIYWKFFYPNKTFYNFIIESYSKPNDFNPEVIKSMPLENEINFYKFRNEKVNTILN
                     MEYPEGNYTQKEKDFWLSKNKDIKEPYLNGYYQGWEYFLLARDLLIFTIMAVCISIAS
                     VFSGEYQSGADSVVLTSKYGKTKVITAKIISSFLFGTIIFTINTIVSIGILLLSFGTD
                     GWNLPLQLTTSSIPYPYTFLQAILLSVLTIYMVLIVLISFTLLLSSYMKSPFPVLITN
                     IILLFVPIFFKYSETNKLYNQILNVLPSNAISFSFTNYVDYKFGNLIIALPTMIIFTC
                     LVLTVVFLPFIKNNFKKHQVN"
     gene            complement(10045..10617)
                     /locus_tag="MJE33_003179"
     CDS             complement(10045..10617)
                     /locus_tag="MJE33_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBY9649095.1"
                     /translation="MFKKIVAMASSIIVSTICLTGCDFNSNDKKDESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKIDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLVARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKAGNVNVRTDVQKSV"
     gene            11003..11737
                     /locus_tag="MJE33_003180"
     CDS             11003..11737
                     /locus_tag="MJE33_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBY9649096.1"
                     /translation="MKQNKYDDDRFFNKYSKMERSTNGLAGAGEWHVLKKMLPDFKGK
                     RVLDLGCGFGWHCQYAVENGAISAVGVDISEKMLKEARNKTQLDNINYICMPIEDINF
                     PKDSFDVVISSLAFHYIQSFEDICKNISNCLSNKGNFAFSVEHPIFTAYGSQEWYCDK
                     DGNNLHWPVDRYFEEGVRRSNFLGEEVIKYHKTLTTYLNTLMKTGFEILEIIEPQPEE
                     KLLDTIPDMKDELRRPMMLLVSAKKR"
     gene            complement(11880..12371)
                     /locus_tag="MJE33_003181"
     CDS             complement(11880..12371)
                     /locus_tag="MJE33_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBY9649097.1"
                     /translation="MNNQQQEVYKKLDELSIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMDKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
BASE COUNT         4566 a         1567 c         1719 g         4548 t
ORIGIN      
        1 tacttttaga atttattata ttatgttaat tttttatatc ttataagaat tgtatttttc
       61 taactataaa aatagtatta tacttatatt taaaaataaa agaattttat tcctattgtg
      121 taaagtgaga atactatttt cttatattgg tataataata gtaaatctaa tatagaaaag
      181 aggtatttct agtatgcatg aaaataaaaa gaatctatta gacggaagct tagctcaata
      241 taatgataca gcagttccaa aaataccagt atttgctggt aacgatataa cttcagaagt
      301 ttattatttt aaacctaatc aagttcttaa tgctcataga caccctaatg gtgaacaaat
      361 atttgtgttc ctaaaaggag aaggtaaaat gaaacttggt gagcatgaat gtgatgtaaa
      421 aaacggcgat actgtatttg taccaacagg agagtggcat gaaattacta atggaagcaa
      481 tgaagaaatg gtagccgttc aaattacaaa aattaatgct ggagcagaat acagaggata
      541 gatatataag tacagttagt actttaatat ctattttatt atataaactt taatctcaaa
      601 tataaaaaga gtcttttaaa aggctctttt tatatttttg ttaattttca ttataaaaac
      661 aagaaaatat ctagctaaaa ctaatatatt ttatttaata ttttttatag cattgtttgc
      721 aaactcagta tttacgatat ctttataagg tgcttcctta tctagttctt tagcttcttt
      781 cataacagtc atcaatttct tcaaaccttc ctcttttaat acaggatttt gagaccatgc
      841 atctattgat ttatatctat ctacaactgt tgttaaatct tctaaagata tatcttcaaa
      901 gaaaggttgc atagccttag ctacttcttt gctagatgct tgttgaatcc ataattgacc
      961 tttataaaca gcatttgtaa atctttgaat taaatcttta ttgtttttca tgtatgatct
     1021 cgtagtagag tatgctgtaa aaggtatttc tccagaatct tttcctatag atgtgactat
     1081 atatccacta ccttcttttt ccattgctgt tgctgtaggt tcaaatgctg ttgtaaagtc
     1141 accctctcca cctgcaaaag ctccagccat tacaccaaat tggtaatcag tctatttata
     1201 acatttaaaa catataatta atctcttggg atattagggt aaagtattaa agaaaaatca
     1261 tcagattttt gattaggttc ttttttatag actgcataat ttattacact tttaagcaat
     1321 atatttttta gataaggttc tttttcctct gtatattcac ttaaaatttt ttcaagttga
     1381 ggaacaattt gtgttttaac tgtatttctt tccttttgca tttcaatatc tatatttgtt
     1441 ttattaatag cttctcttac attttctata cgagataaga gtatttttaa acgttcccca
     1501 aatgtattat tgtcataaat tccttgttct aaaaaatcat gtagtcgttc tttttgtttt
     1561 tctaaagtat ccaattcttt atttaaattt gtcaaggtat gttctaaagt attttcaggt
     1621 ttagagttat ctttttcatc ttttaaagat agtctataat ttactaacca tttatttaag
     1681 acctctaata ttttattttc taatatgtat agtttagtag ctttattttt acaattagta
     1741 caaaccatat atttctcata tccatttgat gtagtattag atttggctat taaagtatga
     1801 ttacagtatc cacattttac aattccagcc aatggatttg ttattttatt gtttggagta
     1861 ggggaattaa tacgtttttt tcttatattt tgtgccttat tccatatgtc ttcacttatt
     1921 attggttcat gctttccttt tacctcaatt tgttcctcaa ttcctcttgt tctactagaa
     1981 tttcttttcc tctcgacttt attccatact atatatccac aatacacttt gttttttata
     2041 atatctctta tacccttttc ataccaagtt ctacctgttg tggtcttata accaagttcg
     2101 tttaaataat tggatatttt acatgcacca tctcctttta aatacatatc aaagattatt
     2161 ttaacaacag ttgctttatc ttcatttatt acaagtactc tttctttatt atttaaatat
     2221 ttaatatcat acccataagg aggagcttga gctataaatt taccctcttc aatacttttg
     2281 atttttcctc tttgcatacg tctagatatt aattttaatt ccttacgagc cataaaagct
     2341 tcaaattcag aatactcctc atcaaactca tcatttaaat catacgtttt tctaggagta
     2401 ataattttcg ttttagattt tttaaatgta tctagtatta acccttggtc ttgcatatta
     2461 ccacgaccca aacggtcaat atccatcact aaaacagcat catagttatt atttttaatt
     2521 tcacctagta attctagcat cttaggtcta tattctatac tttccccaga aacaagttct
     2581 tctttaattt caatgacatt taaattttgt tctcttgcta gttttaaaag agttgtctta
     2641 tgtctactta gagtttcaaa ttcacctcta tgttctgctt cttcatcagc tctgcttttg
     2701 cgtaaataaa ttgccacatt cattaaatca caccccttga atatattata ttcagtaaca
     2761 gattgattat atttaaaaca caaaaataaa ccttcaaaat acaagatata ttttttaggt
     2821 ttatttttat acagtaaatt ttatagtata tttaaaagct tgtaatctaa gtaaatattt
     2881 atactattaa ttttatgctt tcttcatttt ttaattttaa actatttttt tactacaggt
     2941 atccatactt caaatttagc attttgaggg tctggattta agtacagttc aatatcagga
     3001 ccattatcat attcataacc agaagtaggt aaccattctg ttacaatacg cttttctagt
     3061 tcttgtattg cttgtggaca ttgtccttct ccagaaaata ttgcccaagt aaaagctgga
     3121 acagtgtatt cttctaatgt attatctata gattttgtac ttgaaacaga tataaaatac
     3181 ttccattctt ctgaattatt acaaatactt atacctaata caccttgaag ttgactatcc
     3241 atcataggga ttaatttttg aagcgttcca tctaaagcaa ccttttgcca catttgggga
     3301 acaatttcaa aattcttttc tagttcatta tgaagtggct gagatacacc aactatacga
     3361 aatgcttctt tcttttcaat tctataattc atttcttcgg ctcctttaat actaatttta
     3421 aaactgattg gaggaaaggc ttttaaagtt gttatttctc ttttttttat aactgatggg
     3481 gcaacaccat gaatactttt aaatgcacgg ttgaatgctg ttggagaaga gtatccatat
     3541 tttaaagcca catcaattat ctttttatca tcattttgta attctacagc tgctaaagac
     3601 atccgtctac gacgaatata ttcagaaagt ggtacatctg ccatataagc aaacattctt
     3661 tgaaaatgat atgtagaaca acaagctatt tttgccactt gttcatactc aatttctttt
     3721 gttatgtttt cttcaatata gttaattgct ttgtttaatc tttcaatcca ttccataaaa
     3781 tcagctcctt gtaatttata taatatctat tttaaaatag ttaatcctct ctttctatgc
     3841 actaattaac tagcttaata aagaatgaac atgagaaagt ctgtaattaa ctacaaaaat
     3901 gaacaaatag tattgttttt cacattaaag tatatcacag attagtatct aatttttata
     3961 taagataaca gttttaattt attatgaaat ttttataata ttttagctta gacatttaat
     4021 tatatataac attttattca tagttaacta tttttgaagt ttacccttaa tcaagcaaca
     4081 cttagttact caattataaa taatgattga gaataggaca atttttaaga tacataaaat
     4141 ataaagagga ggggatttaa atgccaaata tagaaaaaga cttagatggt acaactacta
     4201 taacatacct caatgacgat ataggtgttc caacagacga gaatggatat aaaatatcta
     4261 tgacaatact acatggaaag acaatctgcc atgtagtatc tcctgaaaca atcttaggaa
     4321 gaaagttcac attagaagaa aaacaagaaa aagatacaat aatatatgga gcaatagaaa
     4381 aaataatggt agacagacaa ttacaaataa aaaattcaaa caaagaagtt gctagtcatt
     4441 agcacttctt taataagtaa agtaggacaa gtatttttta taatattatt gacttatagt
     4501 ctaattagac tataatataa acatagatag tctaattaga ctactattaa aaataaatac
     4561 aataagaatt taagaaagga taataataat aatgattaaa tcatttttaa tgttaggaca
     4621 gtccaatatg gctggaagag gattcataag tgaagtgact ccaatttata atgaaagaat
     4681 acagatgtta cgtaatggaa gatggcaaat gatgacagaa cctattaatt atgacagacc
     4741 agtttctggg gtaagtcttg ctgcatcatt tgcagatgca tggtgctgtg aaaatcaaga
     4801 ggatagaatt gggctaattc cttgtgctga gggaggcagt tccttagatg aatggaatat
     4861 agatggtata ctttttaaac atgctataag tgaagctaaa tttgccatac aaagcagtga
     4921 attgacagga attttatggc atcagggaga aaatgacagt aataatggca actataaatt
     4981 ttattataaa aaattacttt ctattattga aactcttaga aaagaattaa atattccaga
     5041 tataccaatt attattggtg gattgggaga cttcttagga aaagtaggtt ttggaaaaag
     5101 ttgtactgag tatgtattta tcaatcaaga gttacagaaa tttgcttttg aacaagataa
     5161 ttgttacttt gttacagcaa caggtcttac atctaatcct gatggtattc atattgatgc
     5221 aatatctcaa agaaaatttg gattacgtta ttttgaagct tttcataaga aaaagcatat
     5281 tatggaagca ttagctaatg aaagtgagtt gataataccg agtaattcca agacatatac
     5341 aaaaacagaa aaaatatata taaatagtat ggacttggca ctaggaaaga tatcatatga
     5401 tgagtttgaa tctaaactta ttaaacttaa tgataattag ataaaatatt cattaatata
     5461 ctaataaata tagattgaaa gaggtgattt tttgatacca ttattaattt taggattatt
     5521 aaaacaaaat ccaaattctt atggatatga gttattagca ttaatggaaa agaagcatta
     5581 caagtacata gtaaatttca ctaaaggttc tttttattat aatttacagc aacttgaaga
     5641 aaaaaagttt attgaaaaag ttgaaaataa acaatctaat aaaaacagag aaactcataa
     5701 ctacataatt actgaacttg gatgccagga atttgaaaaa ttaatgtata agtatggttc
     5761 taaaacagag tatataaatc tatcatttta cgcaacacta ttatttgcaa atgattataa
     5821 aaatgaaaag ttaatagaat tattggaaac gcaaattaag caaactcaag taagaatttc
     5881 tctattaaat gaatccatag aaaatgacaa aacaaattca cattatttta atagaatgct
     5941 agaaaattct cgttctcacc atttagttaa tattgaatgg tttgagaagt tgctaaatga
     6001 tattagagat tgattttaat aataaaaaaa catacatata tactttatat aaatgtatga
     6061 tatatagatt aaaaataagg cagataattt tcaattgaat atctgtcttt ttgttatata
     6121 agatgttaaa agacacttat actatgtaag taaaatttat aaatataaag ttgttcattg
     6181 ttgaattaca gacacttaag atgaaaagta tgacatttga tataataaaa ttgtttaagt
     6241 attaattatg agtattaatt aatggtatag ataattttaa tataactgta tttataaata
     6301 attataaaat aataaattaa tttttttata ctgacttttt tatagtataa atcgttatat
     6361 ataatgacgg aggtgataat agtgaatgat gctcggatta tatcacaaat aaaagcagga
     6421 aaaaaagaat tgtttgatac tctaataaaa aaatactatg atagcatcta ttgttattgt
     6481 tatcgccatg tagacaataa aatgactgca caagatttaa ctcaagatgt atttgtaaca
     6541 gttataaata atatagaaaa ttataaacat tatggtaagt ttgaaaacta tttatatgtt
     6601 attgcaaaaa acaagtgtaa ggatttttgg aagaaaaaac aaccgatata tttagagtca
     6661 ttagataagg agatatcaaa tcaagaattg tctgatatag aaaataaaat gtttataaaa
     6721 gaagctttga ctaaattacc agaattagaa tttgaagtga taattttacg gtattaccat
     6781 gatttaaaat ttaaggatat tgctaaaatt atggagtcaa gcgtctctgt cacaaagtac
     6841 aggatgagta gggcattaaa aaaactagaa ttatatctgg aaggaggtaa attatgaata
     6901 ataaagaaat aaaaagaagt ctacagaagt ataaggttcc caaatatgat tcttcagctc
     6961 ttgaaaagac tattaaaact atacacgagt taacgcttag taatacttat aaaagaatga
     7021 ataataaaga cttttttata tctcaaattg gttttataag aaaaagaact tggattatgc
     7081 aatttataat actatgtaca atatgtatat ttttatttag tacatcagca gatagtaatt
     7141 cattttataa tactttatcc ttgatttcta tagcatcacc actttttgtg ctgacaaata
     7201 tagaagaatt aacaaaagta tataatagta gtattttaga gatagaattt tcaacaaaaa
     7261 atacattaca taaaattatt gctactagaa tgcttatatt tggaataagt gatttattgt
     7321 ttatgattat tattttggta tctgcaaaac aaatcattga tgtaaacatt ctgtatataa
     7381 taatttatat actagttcct tttaacctaa tgtgtatagg atgtatggaa attataaata
     7441 gaaataaagg taagcataca aattattatt gtatgatata tggtatatct cttgtattat
     7501 taatagttac actgagtaac tttagagata atctatatca atttgaagca ataaatcaat
     7561 ggctaattgt ttttctagtc acagcagtaa cattaatata tgaaataaga aaaacgtgga
     7621 gacatctttg ttcatttgaa aatattatta tctaagtaaa aaataagaat acacagtatc
     7681 aggaggaata gtatggagtt aagtgtagaa cgtttaacaa aacagtataa caataaaatt
     7741 atagtagata aaattgactt taaattaaat gagggtatat atggattttt aggagaaaat
     7801 ggagcgggaa aaacaactct tatgagaatg atttgcggaa tattaagtcc tacatctgga
     7861 gaagtaaagg tggatggtaa aaataatttg gatatgggag aagattttag agatatgatt
     7921 ggataccttc ctcaaaactt tggatactac ccagatttta ctgccaaaga atttatgcta
     7981 tatatagcat cattaaaagg attaatgcct agctatgcaa agagaaaaac acaagaatta
     8041 ttagaaatgg taggattatc agaggtaatt aataaaaaaa taagaacatt ttctggaggt
     8101 atgaagcaaa ggctaggatt tgctcaaaca ctacttaatg acccaaaaat tttaatatta
     8161 gatgagccaa cagcagggct agaccctaaa gaacgagtta aatttcgcaa cctaatttca
     8221 gaattttcta aggaaaagat tgttatattg tctactcata ttgtgtctga tgtagagtat
     8281 attgcagata aaattttaat aatgaaaaaa ggaagatttg ttttaactgg ctcagtagaa
     8341 gaagtaactt caaagataaa agataaggta tggaaatgtg tggtcaatga gaatgaaata
     8401 gattatatgt ctgatagata ttgtttagca aatatccatc attctggtgg agttgcaaat
     8461 cttagaattg taagtgatat aaagccacat gacaatgcat gcaatttaaa accaacatta
     8521 gaagatttat atttatattg tttcaacgaa gtactataaa tatacataag gagataggta
     8581 tgtctacatt aattaaattt gagttaaaaa aaataataaa aagaaagtct acaatagcag
     8641 ttattttagg aagtttattc ttaagcatca ttctttttgc attaatgcca attcaggaaa
     8701 aagctaatga tgaaagtgga aaagagtata ggggtttaca ggctattgag ctaaaaaagc
     8761 aacaatctaa attaaatgag ggatacataa cagataaaaa agtaaaaaac tcaatcagag
     8821 attaccaaaa aatgtttaat aatccaaata acattgtaac aaatgagtct ggattctcag
     8881 attttaagcc agatatttat tggaaattct tctatccaaa taaaactttt tataatttta
     8941 taatagagtc atatagtaaa ccaaatgatt ttaacccaga agtaattaaa tcaatgccat
     9001 tagaaaatga aattaatttt tataaattta gaaatgaaaa agtaaataca atattaaata
     9061 tggaatatcc agagggaaat tacacacaga aagaaaagga cttttggtta tctaaaaata
     9121 aggatataaa ggagccttat ttaaatggat attatcaagg gtgggaatat tttctattgg
     9181 caagagattt actgattttt actataatgg ctgtctgtat aagtatagct tctgtatttt
     9241 caggagaata ccaatctggt gctgattcag tagtattgac ttctaaatat ggaaaaacaa
     9301 aagttataac agctaaaata ataagttcct tcttatttgg tacgattatt tttacaataa
     9361 atacaattgt atctattggc atacttcttt tatcttttgg aactgatggc tggaatttac
     9421 cactacaact tacaactagt tcaattccat acccatatac atttttacag gcaatattac
     9481 taagtgtgtt aactatctat atggtattaa tagtattaat tagttttaca cttttattat
     9541 cgtcttatat gaaatctcct tttccagttt taattacaaa tataatattg ttatttgtac
     9601 caatattttt caaatacagt gaaacaaata agttgtataa tcaaattctc aatgttttac
     9661 catcaaatgc tatttcattt tcatttacta attatgtaga ttataaattt ggtaatttaa
     9721 taatagcttt acctacaatg attattttta catgcttagt tcttacagtt gtttttttac
     9781 cttttattaa aaataatttt aaaaaacatc aagttaacta aaatatatat tataaaaaga
     9841 aggtgtctta aaatgaactt tttagcttat taggcactct tctttgtatt aatttaaata
     9901 tattttagta ttagattaaa tatgttttca tcattttaac aatgaaaaag agccttatct
     9961 ctattttgag atagctctta taaagttaaa tattaaattg ataactataa aatatgtagt
    10021 ttgaatttat aatttgtgtt ataatcatac tgatttttgt acatcagttc ttacattaac
    10081 attgcctgct tttagatttt ctccaatagt aagacctttt tcttttagaa catattcaaa
    10141 agtcattagt ggaactccac cttttctacc acctaatatt tcggttcctt ttaaatcatt
    10201 gtaagagaaa ttaggcattt ttttcctagc aactagaaaa ctaccatctc tttttgtcaa
    10261 ttgagcaaag ttcactgcat aatcgttttt gttttgatta tatacatata tagatgcttc
    10321 aggacccatt aaacctacgt ttgcttcacc tgaaatcaaa gctgccatag ttttatctgc
    10381 accttgggta tttataaggt caatctttat accttcatct tcaaagaatc ctttcgtaat
    10441 agcagcatac tgtggagcat aaaaaacaga gtgagtaact tctgctatag ttactttatc
    10501 cagattttta gacgaagaag attcatcttt tttgtcatta gagttaaaat cacatccagt
    10561 tagacatatt gtacttacaa tgatagagct tgccattgcg actatttttt taaacataat
    10621 aaatacctcc cataagacat acgaatagta tatagtatta aaaatttatt gtagttgtta
    10681 caaaaatgta tctatgctta taatatttct acaaaaactt aataataatt ttcagtttta
    10741 tgtttagttt ttttagtaat gtgtaatatt ggataaatta tattgattat ttataaacta
    10801 agtaataaaa ttagaataat atcacttttt tgtctattat atataaggaa aaatttttta
    10861 ataatataaa tcatattcat ttatattatt aaaaaattta tttatagaaa cttttattat
    10921 tttatataaa agtgataaaa acttatattt tgtgtttagt agagtgttgc taagtgaata
    10981 tatttaaaat ggaggaaact ttatgaaaca aaataaatat gatgatgata gattttttaa
    11041 taagtacagt aaaatggaac gttctacaaa tggattagca ggtgctggag aatggcatgt
    11101 tttaaaaaaa atgttacctg atttcaaagg aaaaagagtt ttagatttag gatgtggatt
    11161 tggatggcat tgtcaatatg cagttgagaa tggagcaatt tctgctgttg gagttgatat
    11221 ttcagaaaaa atgttaaaag aagctagaaa caaaacgcaa cttgataata taaattacat
    11281 atgtatgcct attgaagata ttaattttcc aaaagatagt tttgatgtag ttattagttc
    11341 cttggcattt cattatattc aatcatttga agatatatgt aagaatataa gtaattgtct
    11401 ttcaaataaa ggtaattttg cattttctgt tgaacatcct atatttactg catatggaag
    11461 tcaagagtgg tactgtgata aagatggcaa taatctacac tggccagtag atagatattt
    11521 tgaagaagga gtacgtaggt caaattttct aggagaagag gttataaaat atcataaaac
    11581 attaacaact tatttaaata cgttaatgaa aacaggattt gaaatattag aaattattga
    11641 acctcaacca gaagaaaaac tgttagatac aattcctgat atgaaagatg agctacgtag
    11701 acctatgatg ttacttgtat ctgcaaaaaa aagataagta aatataaata atcattattg
    11761 tagaaaaaaa gttgtagaaa ataattacac taatggagat gattatacca gtggtattaa
    11821 ttacaccagt agtaaggagg caaaagcatt cgttgctttt gccatttttt aagttgcaat
    11881 tatacttcta cgtagtatat ttcgtttcca ttttcttcta taagcttttt catatcttca
    11941 aattttaaaa atacagtagc agtattatca tttggatgta tacctatttt atccattttt
    12001 aataaatcct tatcaaatac aacttttaca acagcctctt tatcatttaa tataccaaaa
    12061 ggagtcacag ctcctttagt aagtttcaag tatttttgaa gtctttcttc tgatgcaaaa
    12121 ctaagaggtt tactatcaat ttgttgacga atatctttta aatctgcttt tttgtcttta
    12181 cctaatacaa ctaaaaaatg ttcttttcct ttagcatcac gaagaaatag gttttttaca
    12241 acaaattcca tatgtaatgt ttttaattct tccatttctt caattgtata aactggtgga
    12301 tgatttacag cctcataagg tatgcttaac tcatctaatt ttttatatac ttcctgttgt
    12361 tgattgttca tgatttcctc ccattagaaa tttattttat
//
LOCUS       DAGSRM010000110        12365 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.110, whole genome shotgun sequence.
ACCESSION   DAGSRM010000110 DAGSRM010000000
VERSION     DAGSRM010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12365
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.110"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            615..1124
                     /locus_tag="MJE33_003182"
     CDS             615..1124
                     /locus_tag="MJE33_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBY9649098.1"
                     /translation="MSKELAINEQIKDKEIRVLSPTGEQLGVMPTKEAQAMANSKNLD
                     LVQISPNANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTK
                     ANNAIKFLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNN
                     MVVIIDPKK"
     gene            1152..1346
                     /gene="rpmI"
                     /locus_tag="MJE33_003183"
     CDS             1152..1346
                     /gene="rpmI"
                     /locus_tag="MJE33_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBY9649099.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAFKKHILTKKSAKTKMNL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            1402..1758
                     /gene="rplT"
                     /locus_tag="MJE33_003184"
     CDS             1402..1758
                     /gene="rplT"
                     /locus_tag="MJE33_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBY9649100.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGVEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            2068..2613
                     /locus_tag="MJE33_003185"
     CDS             2068..2613
                     /locus_tag="MJE33_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649101.1"
                     /translation="MNEKLMDCFVNPIKCKLLIEIFSNNRTTAKQLGEKFQDISKATL
                     YRYLNKMLKDDIIKVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQY
                     MMGFLREFYEYAERKEKDILKDGSGFSVCPVYASIDEIVEVSKKIASILEPLRNNSHE
                     EGRRMHSIGVIITPPKKDEGK"
     gene            3048..4973
                     /locus_tag="MJE33_003186"
     CDS             3048..4973
                     /locus_tag="MJE33_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBY9649102.1"
                     /translation="MKIKFVIMPLVIFLMVLFVGCQSTKDTEVKAKDTEIKILGTGDL
                     HSILTDSMVSYVNEEREKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIKRNGRAEY
                     DDIIKSSKDEVPIVKDMAKLKYDAVVLGDNEFVSNDKQSLDKLVSDFKNNNMPLVSAN
                     IYEQSGENYVQPYVMKNIKTDEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQ
                     ADYKGKLYANDLVEDAKKWIKVMEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTV
                     DGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEINFKLNKENGKWDIKE
                     KSSKLKIIDKEVSIVATADLHSHFSDKLTVDLVNERSNPIEPVVVDAGDFLDPQTDEM
                     TQWYKEWKSIRDNNSDKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLD
                     AVIEDFEQVAISVLSANIYRQSGKNYVKPYIIKDVETSEGNVKVGILGLTIKEVGKGL
                     KNVNLQEQFQYKDKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNPKHPGNR
                     IKELATTVDGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKE
                     KGSWTINDKFSKTIEVE"
     gene            5374..7278
                     /locus_tag="MJE33_003187"
     CDS             5374..7278
                     /locus_tag="MJE33_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBY9649103.1"
                     /translation="MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAEPRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
                     YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFEQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVNVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKV
                     FGKEVNIITTSGLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEW
                     QYNENKGIYKKINEWPIVYLGYDAVVLGSHEFTEDKETFEEKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEEDLSNSSLSKDSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKAIKMK"
     gene            7967..8746
                     /locus_tag="MJE33_003188"
     CDS             7967..8746
                     /locus_tag="MJE33_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HBY9649104.1"
                     /translation="MSYKKIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKIFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVALGLICIPYMQKGSHIMNISSQASFQPLPYQNIYSSTKAFIRNY
                     TRALNVELKSKGINAIAVCPGWMSTNLFKRGIVVAEKGTKNFPGIVSPDVVAKKALKD
                     AKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
     gene            complement(9056..9877)
                     /locus_tag="MJE33_003189"
     CDS             complement(9056..9877)
                     /locus_tag="MJE33_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallohydrolase"
                     /protein_id="HBY9649105.1"
                     /translation="MKLKIVGSGGMSIIPSSFCKCKICEEARQKGGRYERLGPSLYID
                     DIKMLIDTPEDIAVACNRQKISEVKHISISHHDPDHVKGIRIVEKIGCDFITGENNPI
                     GFYALPEVIEDINRTNLDCLKYYGDVINCISINETSHVKINNFDIDLINNNPDRNITF
                     YVIKENEKKVIYACCNPKPFTHSDMYFDADILIISLVSDDGILNDGTKLKDTPFKDEI
                     FTLDEIVEIKNYYRIKKIIVTHIDEMWGKSYGYYSELEKKLDNIYFAYDGMEIIV"
     gene            complement(10611..11429)
                     /locus_tag="MJE33_003190"
     CDS             complement(10611..11429)
                     /locus_tag="MJE33_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBY9649106.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNKTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IETTIKRIGGNDIMKNYDVTIKNFAPKYMMTLRRVIPTYQDEGMLWHQAFLETKDQNV
                     QIEPPKYSKTVFYDTGYKEDYVDVEVQVAVSGKYKDTEHVKFKTVPSVTAATAIVNGN
                     FNQVADACEAIGNWISDNNYDVDGPMFNIYHVSPGNDSNPDNWVTEVCFPVKKK"
     gene            11597..12178
                     /locus_tag="MJE33_003191"
     CDS             11597..12178
                     /locus_tag="MJE33_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY9649107.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCITNGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEDVDVR"
BASE COUNT         4932 a         1352 c         2056 g         4025 t
ORIGIN      
        1 taggacttgt tatgtagtat agtaacatat tagaatataa tgtaggattt gttatgtagt
       61 atagtagcat attagaatat aatgtaggac ttgttatgta gtatagtaac atattagaat
      121 ataatgtagg atttgttatg tagtataata gtatattaga gtacaatata gaatttgtta
      181 tgtagtatag tagtatatta gaatacaata tagaatttgt tgggtagtat agtgttatat
      241 tagaatacaa tataggattt gttatgtaga atatatagag tcatgtagaa tatacatata
      301 taaaaggatt ttgggtataa atttaaaaat taatagcaaa atgagtagta tagacatata
      361 attagtagta ggaagtaatt aaatttttcc taaacaaaaa aaatagttgc aaataatttg
      421 attaagtgat atagtaaata aggtaagaat aaaacaaaga agaagtccgc ttctcacctt
      481 acgccggagg ttagtaaggt aaatataagt taaagcttaa ttttaattta agtttgttta
      541 aatttggtaa cttatattgc ggatgattac gtcatccgct ttttatttta taaaaatttt
      601 ggaggtgtcc tacaattagc aaagaattag caatcaatga acaaataaaa gataaggaaa
      661 taagagtcct ttcaccaaca ggtgaacaac ttggagtaat gcctactaaa gaagctcaag
      721 caatggctaa tagcaaaaac ttagatttgg ttcaaatctc gccgaatgca aatccaccag
      781 tatgtaagat aatggattat ggaaagttta gatatgagca agcgagaaaa gagaaagaag
      841 ctaagaaaaa acaaaaaact atagttgtaa aagaagtaag acttagacca ggtattgaac
      901 aaaatgattt aaatacgaaa gctaataatg ctataaagtt ccttaaaaaa ggggataaag
      961 tcaaagttga acttagattt agaggaagag aattaggaca taaagacata ggtaaagaag
     1021 ttatgcttaa gtttttagat ataataaaag agtttggaga accaacaaaa gcccctgcat
     1081 ttgagggtaa taatatggtt gtaattatag atccaaaaaa atagatgatt aactgagagg
     1141 aggaatttgt catgcctaaa atgaaaactc atagaggtgc agcaaagaga ttaaagaaaa
     1201 caggaactgg aaagttaaag agagctaaag cttttaaaaa acatatatta actaaaaaat
     1261 ctgcaaaaac taaaatgaac ttaagaaaat caactttagt aagtgatgga gatgctaaga
     1321 gaatagcaca attattacca tactaagata cagattgtaa ataataacta tagaagaggg
     1381 atataaaagg aggtcataac gatggcaaga gttaaaaaag ctatgaacgc tcgtaaaaag
     1441 cataagaaaa tattaaaact tgcaaaagga ttcagaggat ctagaagcaa attatataga
     1501 ccagcaaata cattcgtaat gaaagcatta aaaaatgctt atataggaag aaagttaaag
     1561 aagagagact tcagaaaact ttggatacaa agaataaatg cagctgctag aatgaatgga
     1621 atatcttatt caagattaat gaatggatta aaattatctg gtgttgaagt taacagaaaa
     1681 atgttatctg aaatggctat acaagaccca gaaggatttg caaaattagc agaagttgct
     1741 aaagcaaaat tagcttagta ttgaatcatg agatgtaaag tctttgagtt atataaataa
     1801 ttcaaggact tttttatttt ttgaaaaata cttattagtg tcatgtacaa ccaattttat
     1861 aatgataaat aaaataagca atacaataga attttatgag atttattata agaattgtta
     1921 caattattta tgatagctat aaaaagtcat agtaaaaatg atattgatgt atgttgatat
     1981 atgtataaag tttccaatca atcttgaatt aatattatca acatgataat attatcttat
     2041 taataatata aataaggggg acataatatg aatgaaaaac taatggattg ttttgtaaac
     2101 ccaattaaat gcaagttatt gattgaaatt ttttcaaata atcgaactac agctaaacaa
     2161 ttaggagaaa aatttcaaga tatatcaaag gcaacacttt atcgttacct taataaaatg
     2221 ttgaaagatg atattataaa agtggttgaa gaaaataaaa taaggggaac tgttgaaaaa
     2281 gtatatgcta tgaagttaga tatagctgat gatgtgaaca aaattataga tgagaactct
     2341 ggagaagaat atgctcaact gtttactcaa tatatgatgg gatttttaag agaattttat
     2401 gaatacgctg aaagaaagga aaaggatatc ctaaaagatg gttcaggatt ttctgtctgt
     2461 ccagtatatg ctagtattga tgagatagtt gaagtgagta aaaaaattgc tagtatacta
     2521 gaacctctaa gaaataactc acatgaagaa ggtagaagaa tgcattctat aggagtgata
     2581 attactccac ctaaaaagga tgaagggaaa taaatctgat ttacatgggg tagagccaat
     2641 ttatacaggg aatcgtttta tgatttacat atgtatccag agcaaagtat ctctgcttgg
     2701 attgtacttc attgtaccat ttcatccaat gacttggagt ggtacaatga agttttttaa
     2761 tttttcatat taatgctaaa gaaatgaact ttagaccaca tatgcaatta ggttgcatta
     2821 tacaattaga ccacatatac aaaataaaaa tcagtatttt tatagcagat aaattgactg
     2881 tcattcttct taaaaatcat ctattagtta ttgttagcat attgaaagaa attatagtaa
     2941 tctattgaat aaatgaatat tgcctataat catataaact gctatagttt aaaataaagc
     3001 aggacgttaa attttatagt aatctaaaaa tttaagaggg ggattttatg aaaataaaat
     3061 ttgttattat gccattggtg atatttttaa tggtattatt tgtggggtgt caaagtacaa
     3121 aagatacaga agttaaagca aaagatacag aaattaaaat attagggaca ggtgatttac
     3181 attcaatttt gacagactct atggtttcgt atgtaaatga ggaaagggaa aagaataaaa
     3241 atctgcttat ggtagatgcg ggagatttct atggtacaca aagtagagaa atgtgggaat
     3301 ggtcttctgg aaaaaagtta attaatatta aaagaaatgg tagagcagag tatgatgaca
     3361 taattaagag tagtaaagat gaagtaccta tagttaaaga tatggcaaag ttgaaatatg
     3421 atgctgtagt tttgggagat aatgaatttg tttcaaatga taagcaaagt cttgataaac
     3481 tggtttctga ttttaaaaat aataatatgc cattggtatc agcaaatata tatgaacaat
     3541 ctggagaaaa ttatgtacaa ccatatgtaa tgaaaaatat taagacagat gagggcaatg
     3601 taaaagttgg aatactaggt cttactataa aagaagtagg ggaaaccctt ggtcttgagg
     3661 attttgaaaa ggataaaaaa gctagagagc ttggagaaca agctgattac aaaggaaaat
     3721 tatatgcaaa tgatttagta gaagatgcaa aaaaatggat aaaagttatg gaaaaagaaa
     3781 gtccagacat aatagttgca gttgtacact caggggaaga acctaaaaca gatagaaatc
     3841 caggaaatag aataaaagag ttagcaacta cagtggatgg aatagatgca attgtagctg
     3901 gtcatactca tgaaaaaata gatgaacaca aatataaaaa taaatctgga gatgaagtaa
     3961 ttgttacaca accaggttgg catggagata gagtatcaga gattaatttc aaactaaata
     4021 aagagaatgg aaagtgggac ataaaggaaa agtctagtaa attaaaaata attgataaag
     4081 aagtaagtat agttgctact gcagatttac attcacattt ctctgataaa ttaacagttg
     4141 accttgttaa tgaacgaagt aacccaattg aacctgttgt agtagatgca ggcgacttct
     4201 tagaccctca aacagatgaa atgactcaat ggtataaaga gtggaaaagt ataagagata
     4261 ataatagtga taaaacgatt agtagatgcc caatggtcat ggatatgaat cagggaatgt
     4321 atgatgcagt agtacttgga aatcatgaat ttgtatctga aaaagatgaa ttttggtcag
     4381 atgaacgcct tttagatgct gttatagagg attttgaaca ggtggcaatt tctgtgttat
     4441 cggcaaatat atataggcaa tctggtaaaa attatgttaa accatatatt ataaaagatg
     4501 tggagacgag tgaaggtaat gtaaaagtag gaatattggg acttactata aaagaagtag
     4561 gtaaaggtct taaaaatgta aacttacaag agcaatttca atataaggat aaattgtatg
     4621 caaatgattt agtggaagat gcaaagaaat gggtaaaagt tatgaaaaag aaaaaaccag
     4681 atataatagt tgcagttgta cattcaggag aagaaccaaa aaatccaaaa catccaggaa
     4741 atagaataaa agaactagca actacagtag atggtattga tgcaattgtt gctagtcata
     4801 ctcatgaaaa aatagatgag catgaatata aaaataagtc tggagaaaaa gttatagtga
     4861 cacaaccagg tgagcatggt aaatattatt ctaaaattac ctttaaagta aataaagaaa
     4921 agggttcgtg gactattaat gataaattca gtaaaacaat tgaggttgaa taactttata
     4981 aaatttatag ataaagtaaa ttatctgata ttggcatgat atataagata aagtttctaa
     5041 tattagtata atgcataatg tgaaatattt taataataag agaataaaca agacaaataa
     5101 aaatatttta acatgtaaga tatagtatga actattatac taatgcttac tttataattt
     5161 gaacatgtta gaataagtct tatttgaaaa ttttagtgta ctagagtaag tattatttta
     5221 ctagtaaaaa atattgagaa ctatatgtag atttgcttaa aaaggaattt acatatagtt
     5281 cttttcgtat aataaatagt acaatcttat atggtaaaga taaagtgtga tattaaattt
     5341 tattatcatc caaaatctaa ggaggagtct cttatgaaaa taaaatttat tatagtagca
     5401 tcaataatat ttttaatagt attacttgtg gggtgtcaaa gtactaaaga cacagaaatc
     5461 aatgtactcg ccacaagtga tttacattca attattccag ataatttaat ttcctatgta
     5521 gaagaggaga gaaaatatga tgagaatcta cttatggtag atgcaggaga tttttttgac
     5581 atgcaaagtg gtgagatgaa taagtggttt actggtcaaa aattagtaag atttaaagat
     5641 gggataccag agtacaaaaa aatagctgaa ccacgtgaag gagaagttcc attggctaaa
     5701 aaaatggcaa agctgaagta tgatgcagtt acatttggga atcatgaatt tgtatctaat
     5761 gacaagcaga gccttgataa attggtttct gattttaaaa ataacaatat accattagta
     5821 tcagcaaacg tatatgaaca atctggagaa aactacgtaa agccatatgt aatgaaaaat
     5881 ataaatactg acaagggaac tgtaaaggtt ggtatacttg gtctgactat aaaagaagta
     5941 ggggaacgtt caaggtttaa aagttttgaa caagataaaa aggctagaga acttggggaa
     6001 caacctcaat acaaagggaa attatatgca aatgatttag tagaagatgc aaataagtgg
     6061 gtaaatgtta tggaaaaaaa agaaaagcca gatataatag ttgccattgt acattcaggt
     6121 gaagagccaa aaaatcctaa aaatccagga aatagaataa aagaactagc aacaactgta
     6181 gagggaatag atgctattgt agcaggtcat acacatgaaa aaatagaaca gcatacatat
     6241 aaaaataatt ctggtgaaga agtaatagta acacagcctg gagctcatgg taatagtata
     6301 tcaaagatta attttaagtt ggataagaag aatgataagt ggtttataaa agacaaatat
     6361 agtgaattaa aggtttttgg aaaggaagta aatataatta caacttctgg tctgaatcca
     6421 aaattttcag atgaaatggc agttaatttg tttaatgaaa gagatagaga tgttcaacct
     6481 atctttatag atactggaga ttttttaaat actaatacaa aagaaatgac taagtgggct
     6541 aaagagtggc aatacaatga aaataaaggc atctataaaa aaataaatga atggccaatt
     6601 gtatatcttg gttatgatgc tgtagtactt ggtagccatg aatttacaga ggataaagag
     6661 acttttgagg aaaaaaaatc tacattggat tatattatag aaaactttga agaaatgaag
     6721 ataccagtat tatcagctaa tatatatgat gaatctggag aaaattatgt aaaaccttat
     6781 ataattcata atgtagaaac tagggaagga aatataaagg ttggagtatt aggtcttact
     6841 actactgata aggatgaaga agacttaagt aactcaagct taagcaaaga ctcaaagact
     6901 tcgaaaattc ctgaagaaac aggaaagtta tatactaata atctagtaaa agatgcaagc
     6961 gaatgggtaa aagttatgca aaaggaaagt cctgatgtga ttgttgcagt tgtacattca
     7021 gataataata gtactctaaa aaatccaagt agtgaagtca aaaaacttgc aatgtcagta
     7081 gatggaatag atgctattgt tgtaggtcat acgaaaaata aggtagaaga acatatttac
     7141 aaaaataagt ctggggatga ggttatagta acacaatctg gagaagattg ttattcaaaa
     7201 ataagtcttg aactaagaaa agaaaatggc aagtggaata tcataaacaa atatagtaaa
     7261 gcaataaaaa tgaagtgaat ttattgatat ataaaccatt attaacactg atttttataa
     7321 tataataaat actgattcta caatataatt aacactgatt tttataatat aataaatact
     7381 gattctataa tataattaac actacttttt ataatgtcat caacactgat tctataatat
     7441 aattaatact acttttttaa atataatcaa tactgattcc ataatataat taacactact
     7501 tttttaaata taatcaatac tgattctaca atattattaa tactactttt tataatataa
     7561 tcaatactga ttctacaata taattaacac tactttttta aatataatca atattgattc
     7621 tacaatatta ttaatactac tttttacaat ataatcaata ctgattctac aatataatta
     7681 acactacttt ttaaatataa tcaatatcga ttctataata ttaacactac tttttataat
     7741 ataataaata ctgattctat aatataatta acactacttt ttaaatataa tcaataccga
     7801 ttctataata ttaacactat tttttatgct atataacaat tattgaaaat gatataaata
     7861 taatatctca aataatatga atataacatc tgaatagagg gtgttttatt tgtaaaaaat
     7921 aagtttacag tttgagcgta taaacttatg gtaaaggaga ataattatga gttataagaa
     7981 gattgcaatt ataacgggtg ctaatagtgg ttttggtaaa gagtttctaa agttattaat
     8041 aaatgaagaa gaaataacag aaatatgggc agttgcaaga aacaaggcga gattaaatca
     8101 attagtagat gaatttggaa gtaaggtaaa aattttttca aaagatttat caaaaataga
     8161 agaagtaaaa gaaattggta catttttaag taaagaaaat gtatgtataa aataccttat
     8221 aaacaatgct ggttttgcaa aattctgttc atacaatgat ttaagtatag atgaatctat
     8281 taatatgatt gatttaaata ttagtgctgt tgtagcattg ggacttatat gtattccata
     8341 tatgcaaaag ggaagccata tcatgaatat ttcttcacaa gcatcattcc agccattgcc
     8401 atatcaaaat atttatagtt caactaaagc gtttataaga aattatacaa gagcattaaa
     8461 tgttgaactt aagagtaaag ggataaatgc aattgctgta tgtccaggtt ggatgagtac
     8521 aaatctattt aagagaggta tagttgtagc tgaaaaaggg acaaaaaatt tcccaggcat
     8581 agtgtctcca gacgttgttg ccaaaaaagc attgaaggat gcaaagaaaa ataaggatat
     8641 ttctgtgtat ggtataaata caaaatccag tcatttatta gcaaaactat tgccacaaaa
     8701 aatgatgatg aaggtttggt taatgcaaca acatattaag gaataagagt tttggtaatt
     8761 aagtatcagt tggagattca atctgttata tattatgaga agttttgttg agcaaataat
     8821 aattgtgaca aaacggctta tctagtgacg ataagccgtc tataatgtat ataaaatata
     8881 tttttgattt tactttattt agatattgaa gtattaaaat atgtaggact tgaaatttta
     8941 taaagataaa tactatgata ttttgtaaaa actgcgatat gttgatatca catagtttga
     9001 ctatactgtt aaattgaagt tttcttattt tgcattatgc ttattactta atgatttaga
     9061 ctataatttc cataccatca tatgcaaaat aaatgttatc aagtttcttt tctaattcac
     9121 tgtaataacc atatgatttt ccccacattt cgtcaatatg tgtaacaata atttttttaa
     9181 ttctataata gtttttaatt tcaacaatct catctaaagt aaatatttca tctttaaatg
     9241 gtgtgtcttt aagttttgta ccatcattta atataccatc atcagaaact agacttatta
     9301 ttaaaatatc agcatcaaag tacatatcac tatgagtaaa tggcttggga ttacagcagg
     9361 catatatgac ttttttttca ttctctttga taacatagaa ggttatattc ctgtctgggt
     9421 tattgttaat caaatcaata tcaaaattat ttatctttac atgcgaagtt tcattaatag
     9481 aaatgcaatt tataacatca ccataatatt taagacaatc gagattcgtt cggtttatat
     9541 cttcaattac ttcaggaagc gcataaaagc caattggatt attttctcct gtgataaagt
     9601 cacatcctat tttttcgaca attctaatac ccttaacatg gtctgggtca tggtgagaaa
     9661 ttgaaatatg ttttacttcc gatatttttt gtctgttaca agctactgca atgtcttcag
     9721 gtgtgtctat cagcattttt atatcatcaa tatataaact tggtcctaat cgttcatatc
     9781 ttccgccttt ttgtctggct tcttcacata ttttgcattt gcaaaatgaa cttggaataa
     9841 ttgacatacc gccactacca acaattttta atttcaaagt tattacacca ccttctaaaa
     9901 atattagatt atataaataa tctacaatca caactgatta acaaaattat gagttaataa
     9961 attatatcac atgtaaatat gaaagaatag tatgaaattt ttataagcat atattaaata
    10021 tttctcaatg aaattgactt tgtatatgaa ccgaatgact tattcaatat ggataagaca
    10081 tttttcttgt aataagatat gattgtccta taaggtttat gtaattttga tataatttga
    10141 tataatatat tgattaacaa catacatgtt attggtgagc atatttctat agaatggaat
    10201 atataatttt ttatatttat cattgtatta ttgtacaaag aaagttaaaa agagtagagt
    10261 acaaaggaat tatttgtact ttactctttg ttttgttaaa aactaattta tattagtgat
    10321 tcaaatattt aactagagta tgagggttgt atgtaatctt ataagtaatt tatctgacaa
    10381 attaagataa tatcccaatc aaattctagt catttaaaca taacatcaca agtgatttat
    10441 ctgacaaatt aagataatat cctaattaaa ttgtagatat ttatacataa cactaaaagt
    10501 aatttgttta acaaattaag ataatatcct aatcaaattg taggtattta tacataacac
    10561 taaaagtaat ttgtctaaca aattaaaata ttatccaatt aatataaaaa ttacttcttt
    10621 ttaactggga agcatacctc tgttacccaa ttatcaggat tactatcgtt gccaggactt
    10681 acatgataaa tattaaacat aggaccatcc acatcatagt tattatctga tatccaatta
    10741 ccaatagcct cacaagcgtc tgcaacttga ttgaaatttc cattaacaat tgcagttgca
    10801 gcagtcacac ttggaacagt cttaaattta acatgttcag tatctttata ctttccagat
    10861 acagcaactt gtacttcaac atctacataa tcttctttat atccagtatc ataaaaaact
    10921 gttttagaat actttggggg ttcaatttga acattctggt cttttgtctc taaaaatgct
    10981 tgatgccata acattccttc gtcttgataa gttggtataa cccttctaag tgtcatcata
    11041 tattttggtg caaaattctt tatagttaca tcataatttt tcataatatc atttcctcca
    11101 attcttttaa tagtggtttc tatttttaat agttttttct gtgtatcttc taattgttct
    11161 tttacttgag aatggtggat atttagatat tttatgagac tttctttatc gttatattct
    11221 gtcaaaattt cttttataga atacaagcca aagcccatat cttttagtgc atggattctg
    11281 tttgtagtag aaagttggtc tgcactatag tatctatatc cattagtttt attcgtatga
    11341 gaaggagtta aaagtcctat ttcatcataa tgacgtaaca tacgaatact aattttactt
    11401 agctttgaaa aatcgcctat cttaaacatg tttttgtctc ctttataaag gtacttttag
    11461 cttaatatta atataaacca tatcataatg ttagagtcaa ctaaaattat aattaaatta
    11521 tattgacata tgatgaaaaa aatattatac tgaacatgtt cagaactgaa aatgttaatt
    11581 gttttggagg tctattatga aaagtaatga taaagatgct agagaacgta taattgaagt
    11641 tacattgaat cttttaaatg aagtagatga tattgaggag attacagtta gaaaaattgc
    11701 tgagcgtgca aatgttggag ttggtttaat aaattatcat tttaaaacaa aggataattt
    11761 gttgagtact gccataggag atgtgatgtc aaatataata gcagagctat atgacgatag
    11821 tgtatatact ctaagaccta ttgaagattt aaaaaattta ttaaaaaaat tgtgtgatac
    11881 tggattacat tatgaaaaag tgcttccttt tgtattgaat caatgtataa ccaatggaga
    11941 tatgcaagca gaacttgata ttgtccctat gcttcgtaaa atttttggaa ataaaaaaga
    12001 tgaaatgagt ctaagaatca ttgccctgca aattatttta ccaatacaga tttctgcttt
    12061 atctacagaa tcatttcagt tatacagtgg tataaatata aagaataaat atgagcgaga
    12121 taaattcatt gatattttaa tagaaaatat tattggagag gatgttgatg taagatgaaa
    12181 attataacta ttggttcaag tcttatcact gttttacttt ttctttcaac tatggtttgt
    12241 gggttctgga ttaagaataa taaggtaaca gatgcaagtt ctattaagtt ccatatgaat
    12301 agtgctattt ttacagggat atttttattg atttccacaa tccttttaat tatatatata
    12361 aaaaa
//
LOCUS       DAGSRM010000111        12199 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.111, whole genome shotgun sequence.
ACCESSION   DAGSRM010000111 DAGSRM010000000
VERSION     DAGSRM010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12199
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.111"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            315..1706
                     /locus_tag="MJE33_003192"
     CDS             315..1706
                     /locus_tag="MJE33_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBY9649108.1"
                     /translation="MSSTESVSKKSGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSIAMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEK"
     gene            1959..3752
                     /locus_tag="MJE33_003193"
     CDS             1959..3752
                     /locus_tag="MJE33_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBY9649109.1"
                     /translation="MNIKDRLSGLRKFMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDGIWPDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILNELAKENVQIKPYNDVYEFVKNIDKTEKVLLDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEVELENIRNSHVKDGVAFTKFMYWLKKNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKAKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIVPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(3833..5215)
                     /locus_tag="MJE33_003194"
     CDS             complement(3833..5215)
                     /locus_tag="MJE33_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBY9649110.1"
                     /translation="MSVLKTQEKLKQPRDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLAWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSKMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGLSISMYGYRYVFLFSSITVLIGLFFFIFGNRKFYKPLN
                     NDPINGRESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVIQTGTDPNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISFLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLI
                     MNKKLVELSN"
     gene            5473..5820
                     /locus_tag="MJE33_003195"
     CDS             5473..5820
                     /locus_tag="MJE33_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBY9649111.1"
                     /translation="MIRKKSELQVEVKENLNGGIGKVKLENIIQNEELKGKGRLFKRV
                     ALPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EEDLEFVALVLYM"
     gene            5838..6872
                     /locus_tag="MJE33_003196"
     CDS             5838..6872
                     /locus_tag="MJE33_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649112.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKETNSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLKESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARSKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVIWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(6921..7970)
                     /locus_tag="MJE33_003197"
     CDS             complement(6921..7970)
                     /locus_tag="MJE33_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBY9649113.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEVGKLVSKETKIDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MVGFAVMSFRENGVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSAGKGGISMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
     gene            8245..9780
                     /locus_tag="MJE33_003198"
     CDS             8245..9780
                     /locus_tag="MJE33_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649114.1"
                     /translation="MIFVKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKNPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNKVSAVGVGKATIYAKCGNKEIERTFRIQARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPVERAISLSSNGNVVGL
                     REGKDRVRITCGDKSKVITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINESDLGKSNKVYATIEVDMKGNQNIDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMRVENISANVDRDNNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFTLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNSSQNSDVI
                     NVR"
     gene            complement(9965..10651)
                     /locus_tag="MJE33_003199"
     CDS             complement(9965..10651)
                     /locus_tag="MJE33_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HBY9649115.1"
                     /translation="MKTIGLIGGMSWESTITYYQVINTVIKERLGGLHSSKCILYSVD
                     FQEIEECQSSGNWEKSAKILADAAIKLQEAGADFIVICTNTMHKVSDKIQESIHIPLL
                     HIADVTATVLREKEIKKVALLGTKYTMEQDFYKNVIINNGIEVLIPNEEDRIIVNDTI
                     FNELCLGIISESSKKAFLSIIDKLGKQGAEAVILGCTEIGLLIKQNDTSIPLFDTTVI
                     HAIEAALSSI"
     gene            10776..12158
                     /locus_tag="MJE33_003200"
     CDS             10776..12158
                     /locus_tag="MJE33_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBY9649116.1"
                     /translation="MPINSFENYPMTWKPKLDNRKPPIYKTLAMLLEEDIKRGNLNPG
                     DKLPPQRELADFLDLNLSTITRAFKLCEEKGLICAKVGKGTFISSDVNVSNTLLYQTE
                     SKDIIELGTVHPPYEQNTYIIDFIKNVLKQPEMDRFLQYMSPSGTYMQKKSIAKWIER
                     NNVYTSEENILLSTGGQNAICATLLGLFKAGDRIATDSLSFSGIKSIAKMIGIQLVPI
                     PQENNEISIEYLESYCKNENIKGIYLIPDYHNPTTHTMSDLSRKKIAKIAKQYNIIII
                     EDAINSIFRDGIQTPIFSLASDNTIYIFSTSKFLCAGLRVAFVVAPKRYIENLENALY
                     NMNLMVSPFTAEIVHRLLYSPIIDKIIEEKRDAIIERNEVADKVLSDYNLIGDKNCSF
                     RWLLLPDELDGKSFEISAKNLGVQVYCAERFSVGNSTVPKAVRICVTAPKDVEELEKG
                     LNIIKSLLKS"
BASE COUNT         4664 a         1480 c         2029 g         4026 t
ORIGIN      
        1 catgcaaaaa ggtattaaaa agaaatattt ttaatttgaa ttttttcaat aaaaatttac
       61 aaaaatgaaa aaataaacaa aaaagaattg aagatttaag ttgttattta aatatatata
      121 tattaagatt ttctgctgaa gatactaaaa aaataatatt tttcataatt ttaaaaataa
      181 taataaaaaa atgaaaatac aggctataat ctattgagta aacatagaaa agatgatatt
      241 ataagtatgt gctaaaatgg ttaaagcaat attaatatta aatttaacta taaaacaaaa
      301 gggggcgttt ttttatgtca agtacagaaa gcgtatctaa aaaaagtggt catccgaaga
      361 gtttttggct tatttgcatg acaattttgt gggaaagatt tagttattat ggtttaacag
      421 ctttattggt attatttttt acagcaagtg tagctcaagg tggtttaggt ctttctactc
      481 aagaagcaac atctatgttt ggcttattta caggtcttat atatcttaca ccattagtcg
      541 gtggctggtt agcagataaa gttttaggtc aacaaaaatg tatttctata ggtgcacttt
      601 taatagcaat aggagatttc gtactatttg cttcatctac atctattgca atggtttggg
      661 taggtctatc aataattatt ttaggaaatg gtttttttaa atcaagttgc tcaaatatag
      721 ttggggatat atatcctaaa actgatgtgt caagaaaaga tgcagcatac agtttattct
      781 atatggcagt aaatgtagga gcatttatag ctccaataat atgtggattg ctttctgata
      841 atgtatttgc agtaagagga gcaagtggag atatagtatc ttatggatat aagatggcat
      901 ttttaatttg tggtataggc atgatgttag gtatgattat atttacactt cttgcaccaa
      961 aattattaaa tgaagtcgga aaatatcctg tgactaaagg taataaaggt gaaaaaattg
     1021 agtatggacc acttactaag ttggagaaaa ataggataat agcaatggtt attttatttt
     1081 tatttgtggt cttattctgg acagcatact atcaaacaca aagttctttt atgatttatg
     1141 taagggataa tgtaaataga actctatttg gatttgaaat gccagtagca tggcttacat
     1201 cactaaatgc tatattatgt gtggctttat gcccagtatt agctacccta tgggttaaat
     1261 tatctcatac caaaagaggt gatttctcta ttccagttaa aatgggatta ggaatgataa
     1321 taatgggtgt tgggtttata gttatggtat ttgctacatt agctaatggt ccagatggaa
     1381 tggtaaaggc tagtatattg tttatagtat tgacttatat atttacaaca gtaggtgaat
     1441 tattcttatc tcctatagga cttgcaatgt tcaataaatt agctccagct aagtatgcta
     1501 cgttgtctat gggagcctgg tatctaacaa gtttttttgc aagtttatta tcaggaaaga
     1561 ttgctggttt tacagccaca cttggttttt tacagatatt tggaggaata gcaacagttt
     1621 taataatatt tggtttaata ttattgggaa taagaaatgt attaacaaga ttaatggcta
     1681 tggatttact aacaaaagaa aaataattca tgacgaatta ttgtaaataa atgaattact
     1741 ttaaataaag gaagttacat ttaaatataa aattaaatgt aatttcctct ttgattatga
     1801 aaaaaagctt tacacaaata gatattataa ttaaaattat aattgtgata attagagtgt
     1861 ataagattta tataaattat acaaaaaata tttatgtaat aaactttaca tacttatata
     1921 tttagttatt caaactatta tttcgaggag gtcttgttgt gaatataaaa gatagattaa
     1981 gtggattaag aaaatttatg gaagaaaaaa atatagatgc ctatatgatt ccatcttctg
     2041 ataatcacca aagtgaatat gtaggagatt acttcaaatc aagagaattt atatcagggt
     2101 ttaatggttc agcagggacg gttattgtga ctaaagacga agctgggtta tggacagatg
     2161 gaagatattt tatacaggca gaatctcaat tagaaggaag tacaataaaa ttattcaaaa
     2221 tgggacaaga aggttgccca actacagatg aatatttata taaaaatatt cctgaaggag
     2281 gaactttagg atttgatggt agagttatat ctgctagaga aggagctaca ttagctgaaa
     2341 aattatctaa aaaaggtata aagatagaat atcagtatga tttaatagat ggtatatggc
     2401 cagatagacc tgctttatca gatagtaaag catttttatt agatgttaaa tattgtggag
     2461 aaagttttag ctcaaaatta gctagattaa gagaaaaaat gagtgaaaaa ggtacttcta
     2521 ctcatgtaat aactactctt gatgatatag catggttatt caatataaga ggtggagatg
     2581 ttaaatataa tccagttgta ctttcttatg cagtaataac tttaaaagaa gtttatctat
     2641 ttgttgatga aagtaagtta aatgaagaaa tactaaatga actagctaag gaaaatgttc
     2701 aaataaagcc atataatgat gtgtatgaat ttgtaaaaaa tatagacaaa actgaaaaag
     2761 ttttattaga tggaactaag cttagttata caatttacaa taatatacct tgtgaagttg
     2821 agaaagttga tgagttcaat ccagtaatgt tttttaaagc tcaaaaaaat gaggtagaat
     2881 tagaaaatat aagaaattca catgtaaaag atggtgtagc tttcactaaa tttatgtact
     2941 ggttaaagaa aaatgtaggt aagatggaaa tcactgaaat aagtgctact caaaaattag
     3001 aggacttaag aagagagcaa gaaggtttct ttgagccaag tttcaataca attgctgcat
     3061 ataaagagca tgctgcaatg atgcactata gtgcaactcc agagtcaaat tataaattag
     3121 aggcagaagg attattttta gtagattcag gtggacaata ttatgatgga actactgata
     3181 taactaggac tacagtttta ggacctataa gtgatgaatt aaaactacac tttacttctg
     3241 tagcaagagg aatgataaat ctttctaagg ctaagttctt gcatggttgt agaggatata
     3301 acttagacat actatctaga agttgcatgt ggaatatggg aattgattat caatgtggaa
     3361 ctggacatgg tataggattt gtgctaaatg ttcacgaagc tccaaatgga tttagatgga
     3421 gagtagtacc tgaaagattt gatagtgcag ttcttgaaga aggaatggta actactaatg
     3481 aaccaggtat atatatagaa ggttctcatg gtataagaac agagaatgaa attgtagtta
     3541 gaaaagctga aaagaatttc tatggtcaat ttatggaatt tgaagttgtt acgttagctc
     3601 ctattgactt agatggtata gtaccagaat taatgaataa ggatgaaaaa gattatttaa
     3661 actggtatca taagttagtt tatgataaaa tatcaccttt cttaactgat gaagaaagag
     3721 aatggttaaa agtttacact agagctatat agtttgtact agatacaata aaaatcgagg
     3781 tattataatg cctcgatttt ttattgtata ttttattaac tttaaaaaat ttttaatttg
     3841 ataattctac taatttttta ttcattataa gtagtataat tccaaatatc aataaaaatg
     3901 atggtatcac tatgaagact tgtaacatac caagttggtc tataatagct tgcgtatagc
     3961 cagataattt atttgctgca aatttgccta aaaaccaaac acccattagg aatgaaatat
     4021 acttttcagg cgaaagttta tttaccatac catacccgac tggagaaaag caaatttctc
     4081 ctatactttg gaatgttaaa aatccaacta cccaaaatac acttgctttg tgaattgaat
     4141 tagggtctgt tcctgtttgt ataactgcta ctatcataaa caaaaatgct atggctaata
     4201 gtataaaacc taagctcatt ttttttggca cacttaaatc gcctttttta gtttgagata
     4261 attttaccca tactaaagca gagataggtc caagaataac acaaaggaga ccattgtatg
     4321 aatctatcca tgaagtagga atttgaaaat tgccaacagt aagatttatg tgttcctttg
     4381 tatagagtgc aatagacata gaggcttggt tatatgctat ccaaaaaaat actgtgaaaa
     4441 aagctaacac caatattact attattcttt ttttctcaat caaagttaaa ggtcttgaag
     4501 cgagcatttt cttatattct agtgattttc ttatagactc tctcccgtta atagggtcat
     4561 tattcaaagg tttataaaac tttctgtttc caaatataaa gaagaataaa cctattagaa
     4621 ccgtaattga tgaaaataaa aatacatatc tatatccata catacttata gataatccgc
     4681 ttttcatagc aaaccattca ttagcaacta aaccgcatat taaagggcca aataaagtac
     4741 ctagattagt agccatatac gtaatagaga aagctccatc ttttcttggg tcatcattgg
     4801 tatataagtt tccaaccata gtatgtaagt ttcctttata aaaacctatt cctaatccag
     4861 ataaaaatat catgatgttt atatccatct tactatttgc tatgtatcct agaaaataac
     4921 caattgaaac cattagcatt ccaagtataa tacagttttt gggacctaaa aatctatcac
     4981 tgacccatcc accaataact ggagagaaat aagtccatgc taaaaaagat gcaagcattg
     5041 atgcggattc tgctggtgtt aaccctaaac cacctttgga gacttgttca gctaaaaaca
     5101 ataataatac caatttcata ccatagtatg caagtcctac aaaggtaaat attgaacagc
     5161 agaaatataa atctctaggt tgtttaagtt tttcttgcgt ttttaaaact gacatatgtc
     5221 gagaacctcc agataaatag tttaacaaaa ttttaccata ctatatgagt atatgttttt
     5281 ttcatataga cgtcaacttg taagataatt taattaagtc gaaatatatg taaaaatagt
     5341 atcatttttg tgtattcagt aatatttttt tatgataata tatgttatta ttatattaaa
     5401 atacaaacat atttatggaa aggatatgcc aatttagtag ttagattgat tgtttggcag
     5461 gtgggatgag ctatgataag aaagaaatct gaattacaag tagaagtaaa agaaaattta
     5521 aatggtggta taggaaaggt taagcttgaa aacataatac aaaatgaaga gcttaaagga
     5581 aaaggaagac tatttaaaag agtagcttta ccagtaggaa gttcaattgg tgtgcatgac
     5641 catacaacag attttgaagt atattatata ctaaagggaa aaggtaaagt atttgataat
     5701 ggggaatttg tagaagtaaa tgagggtgat gtagtgtata cagctgatgg cgaaaaacat
     5761 tctatagaaa atataggaga agaagatctt gagtttgtag cactagtttt atatatgtaa
     5821 aatatggagg aggagtcatg acaggcaaga tagaagaaat taaggacaag ataaatagat
     5881 tttgcaactc caatttaaat caagaataca aagatataag ctttaaaatt ttgcaaaacc
     5941 ttttagataa taataaagag ataattcatt cttctagagc tgatatatgg tcttcagcta
     6001 tactaaatat agttttagaa caaaatcttt tatacaataa gaagcatcca ctacatataa
     6061 caaaaaaaga gttttcaaaa caaataggtg tttctttaaa tactataaat aataaatcgt
     6121 ctttaataaa agatgtttgt agtattgact ttttaaatca agatactata gctatttcta
     6181 atgtagaatt ttgggtaaaa gagactaatt ccaaatcaaa gtctgtagat ttagatttag
     6241 aaaagaagaa aatactatat aaaagatata ttaagctatc acaagatgcg aatagtgata
     6301 ttgaatcaat tagatatatg gaggaagctg taaatatagg taaaagtatg attactaaag
     6361 aagataaaga acttggattt tggaaaggtt tatctaccag agcctatatg atagcacttg
     6421 aatcccttgc tagaaagtta gaaagtatta ataatctaaa ggagtccaaa aaagtattag
     6481 aatatttaat agaaattaat cctagtgatg agcagggaat aagatacaaa ttattaaatg
     6541 tattaattag attaaatgat agaacttcaa taaataaatt atttgaactt tataaagaag
     6601 aaaaatctgc tacatggatg tacagtaaag ctctttatta ctttaaaaat aaaagtatgt
     6661 ttttggcaaa tgattctatt aaggttgcta gaagtaaaaa taagtacgtt ggattatatt
     6721 tgttagattg gcgaaatgct tttggtagag aatttgttac agaggaagaa aaggcagaag
     6781 ctgtctatta ctatgatgaa aacatagtga tttggaatga agttaaaggt tctatggatt
     6841 ggctattaaa aaaaatgttg gaattttcat aaaaaaaact actccttact tttatataag
     6901 taaaaggagt agtttttaat ttatacatct agttttaaat cttcaggttt tattagacct
     6961 ttttttctca taaattctga aatcaacaaa gttacaatag caggaagtat aaaattaagt
     7021 aaaagtatac caacccacat agatatacct ccttttccag cactttgcat agcagttact
     7081 gttccaaatt gtccaaccaa tccacaagta cccataccag aaccaatagg aatattctcc
     7141 attttgaata ttgttgtaga taatggtcca agtaatattg aagctacagt aggtggtatc
     7201 caaattttcc aattttttat tatattaggt acttgcaaca tagatgtccc aagaccttgt
     7261 gccacaaggc caccaactcc attttctcta aagctcataa cagcaaagcc taccatttgg
     7321 caacaacaac ctacagttgc tgcaccacca gcaagaccac ctaaacttaa catcatacaa
     7381 atagctgcac tacttatagg taatgtaaga gctattccaa caagtgcaga gactaaagct
     7441 cccattatta taggttgcat agttgtagct tgcattatta tatttccaaa tgaagtcata
     7501 aaagcagaaa cagctggacc aataagttca gctacaagta ctccaattag tattgtcaca
     7561 gcaggtgtaa ccaatatatc tatttttgtt tcttttgaaa ctaattttcc aacctctaca
     7621 gcaaaaagtg tagcgacaaa tgctccaaca ggtcctccta attcattacc acaagctcca
     7681 acaagtgcac aagaaaatag tacaagtgga ggtgcttgaa gtccatacgc aatagccact
     7741 gctatagctg gaccagtaac ctgtttagca agagggctta taacttcagt aaaaagagaa
     7801 atattgaaat tatctcctaa tgtattgaaa attgttccaa ttaaaaggga cgaaaaaaga
     7861 ccgagtgcca tataacttag tgcgtcaata agataccttt gaacagaaat ttcaatattt
     7921 tttcttttag caaatgattt gaaatttctt tttttataat tgatttccaa tttaaaatcc
     7981 tcctcatata atataagtat aatttctttt taattaatta tataactttt cagctgacaa
     8041 gacaaatgtt atttcactaa aattagacta ttgtaatagt aagaaattaa cattaatttt
     8101 agttttaaat tttaaattta tggtttataa ttagtatagg gatattctga aatataataa
     8161 ttttccagtt tatcccaata tttaacttag gtagattgct tacaaaacaa atgattatat
     8221 attaggctaa aaggaagtgg atagatgata tttgtaaaaa ataagaaata cagaaaaatt
     8281 gccataatta tatctattcc tatattgctt gttgcgcagt actacctttt taaatttgga
     8341 atacttagac caatggtaaa gggagtagaa attgaaattg tagatgggga atatgttaaa
     8401 gatatagata aatatgtagt aaaattaaat gaaacagtta ctttgtctac aggtgaatat
     8461 attaaggttc cttcgtatgc taaaaatcca aatatttggt ttaatgtatt ggataactca
     8521 gggactttaa aaatagatgg aaataaaatg acagcattaa aggttgggta ttcttctgtg
     8581 ggtatcatga aaaattcaag agtattaaaa aaggctacta taaaagtagt agacccagag
     8641 gtagaatcac tagatatgga gattaatggg gatttaaaac atgttggtga tagtgctact
     8701 atagaaagta caatagaagt tgattatgat aagtttaaaa aatcatataa gccagtttat
     8761 aagagtagca atgaaaatat tttaaaaatc aaaggaaata aagtaagtgc agttggagtt
     8821 ggtaaagcta caatatatgc taagtgtgga aataaagaaa ttgagagaac atttaggatt
     8881 caagctagag ttgctgaaat aaatatggat gatataaaaa tagaagttga ccagaatgaa
     8941 aaattgaaac cagatataat aacttctcca cctggtttag aaccaccaaa aatatattat
     9001 gagttagtag attcaaaatt acctgtagaa agagcaataa gtttaagtag taatggaaat
     9061 gttgtagggc ttagagaagg taaagatagg gtgagaataa cttgtggaga taaatctaaa
     9121 gtaataacag taacagtagt caaagaaact ataacgaatg actatataca aaacttggtt
     9181 ggttcgcata aaatagtaaa caataaagtt attattaaac ttgaatggga ttatatgaaa
     9241 gatgtctttg attatgatat atatcttaaa gataagttgt caggagaatc atttaagaaa
     9301 attaaatcta taactataaa tgaaagtgat ttaggtaagt ctaacaaggt atatgcgact
     9361 atagaagtag atatgaaggg taatcaaaat atagattttg atatttatgt cataggtaaa
     9421 aaggattcag atacaactaa gccaagtaat gtaataaata ttaccaatgg aaatggaaat
     9481 tcaaataatg ataataatat taaagatatg agggtagaaa atatatcagc taatgttgat
     9541 agagataata atgtcattag agttaactgg agtagtatat ctcataagga ctgtacttac
     9601 agtatatatg taaaaaataa tactaatgga gatggagaat ttactctata tcaaagtgat
     9661 atacagggaa atgagcatac tataagtatt cctgaaggag atttaaatct agatatttat
     9721 gttgttgcaa attataatgg aaatagctct caaaatagtg atgttataaa tgtaagatag
     9781 atttgtatac aaattgaaag tgttgagttt atttaaatat taaattaaga aaaaggatag
     9841 tatagggtaa ttatactatc cttttttgtt acaatatata ggtttaaaat ctctagtaat
     9901 ataaattata aaaagtctta tttacacaaa tctatagata ttttttttat taatcaatat
     9961 tgtcttaaat tgaacttagt gcagcttcta ttgcatgtat tactgtcgta tcaaatagtg
    10021 gaatagaagt gtcattttgt ttaattaaaa gacctatttc tgtacatcct aatataacag
    10081 cctctgcacc ttgtttgcca agcttatcta taatagataa gaaagctttt tttgaagatt
    10141 cagatataat tcctaagcaa agttcattaa atattgtgtc atttactatt atacggtctt
    10201 cttcatttgg aattaagact tcaattccat tattaataat tacattttta taaaaatctt
    10261 gttccattgt atatttagta cctaataagg ccactttttt tatttctttt tcccttaata
    10321 ccgtagcagt tacatctgct atgtgaagta atggaatatg tatactttct tgtattttat
    10381 ctgatacctt atgcatagta ttagtacaga taacaataaa atctgcccct gcctcttgta
    10441 atttaattgc agcatcagct aaaattttgg cgcttttttc ccaatttcca ctagattggc
    10501 attcttcaat ttcttgaaaa tctacactgt aaagtataca tttagaagag tgtagtccac
    10561 ctaatctttc ttttattact gtattaataa cttgataata tgtaatggta ctttcccaac
    10621 tcataccacc aattaagcct atagtcttca tattatacct ctcttattta tttattttga
    10681 aatagctatt tacaaataca agtatagcat ctaaaataga agcatacaat aataaaattg
    10741 tatggagtac aattataatt aagaggagta attatatgcc aataaattct tttgaaaact
    10801 atcctatgac atggaaacca aagttagata accgaaaacc accaatatat aaaactttag
    10861 caatgttatt agaagaagat attaaaagag gaaatttaaa tcctggagat aaactgcctc
    10921 cacaacggga attagcagat tttctagatt taaacttaag tacaataaca cgagcattta
    10981 aactttgcga agaaaaggga ctaatatgtg caaaagtggg aaagggaaca ttcatttctt
    11041 ctgatgtaaa tgtaagtaat acattattat atcaaacgga atcaaaagac ataattgagt
    11101 tggggacagt tcatcctccg tatgagcaaa atacatatat tattgacttt attaaaaatg
    11161 tactaaaaca accagaaatg gataggtttt tacaatacat gtctccatct ggcacatata
    11221 tgcaaaaaaa gtcaattgca aaatggatag aaagaaataa tgtatacacc agtgaagaaa
    11281 atatattatt atctacagga gggcaaaatg ctatatgtgc aactttacta ggattattta
    11341 aagctggtga tagaattgct acagattctt taagtttttc tggaattaag tcaatagcta
    11401 aaatgatagg tattcaatta gtcccaattc ctcaagaaaa taatgaaata tcgattgaat
    11461 atcttgaaag ttattgtaaa aatgaaaata taaaaggcat atatcttata ccagattatc
    11521 ataatccaac gacacatact atgtctgatt tatctcgtaa aaagatagcc aagatagcaa
    11581 agcaatacaa tataataatt attgaagatg ctataaatag catttttaga gacggtatac
    11641 aaactcccat attttcttta gctagtgata atactatata tatttttagt acatctaaat
    11701 ttttatgtgc tggtcttaga gtagcatttg tagttgctcc aaaacgatat atagaaaatt
    11761 tagaaaatgc actatataat atgaatctaa tggtttctcc atttacagca gaaatagtac
    11821 accgtttatt atattctcct attatagata aaattataga agagaaaaga gatgcaatta
    11881 tagaaagaaa tgaagttgct gataaagttt tgtcagatta taatttgatt ggtgataaga
    11941 attgcagttt tcgttggtta cttcttcctg atgaattgga tggaaaatca tttgaaattt
    12001 ctgcaaaaaa tctaggagtt caagtatatt gtgctgaacg attttcagta ggaaactcta
    12061 cagttccaaa agcagttcgt atttgtgtaa cagctccaaa agatgtagaa gaattagaaa
    12121 aagggcttaa tataataaag tcacttctta agagttaaat ttattttact attatttaat
    12181 taaataaata aaaaggcgg
//
LOCUS       DAGSRM010000112        11919 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.113, whole genome shotgun sequence.
ACCESSION   DAGSRM010000112 DAGSRM010000000
VERSION     DAGSRM010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11919
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.113"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            <1..430
                     /locus_tag="MJE33_003201"
     CDS             <1..430
                     /locus_tag="MJE33_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBY9649117.1"
                     /translation="NWADEHRIGVAQGVDEEIIEGLRANFVGECTEVGMYLAMSRQAD
                     REGYPEVGEAYKRIALEEAEHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKL
                     AKKAKELGLDAIHDTVHEMCKDEARHGKAFLGLLNRYFGK"
     gene            690..1298
                     /locus_tag="MJE33_003202"
     CDS             690..1298
                     /locus_tag="MJE33_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBY9649118.1"
                     /translation="MVDNLIKYIHKSDVAIVVLHEIYGINSFIEDVCQKYYKYGYDVF
                     CPELLGEKRVFPYSKVQEAYDFFINNVGFDIYKKVEKLINDLKSEYKYVFVAGYSVGA
                     TIAWRCSEDSFCDGIICCYGSRIRDYMDVIPKCPTLLVFAKHDSFDVDSISCQLSRKQ
                     NIQIEILDANHGFIDRYSNNYSDIQTKVFTIKEKSFLSKYLA"
     gene            1625..4522
                     /locus_tag="MJE33_003203"
     CDS             1625..4522
                     /locus_tag="MJE33_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBY9649119.1"
                     /translation="MKAFKKHFLILSLIFLLIISCFNNILCFADESENSKKIRVGYCD
                     DYGIISSSKEHSYTGYGYDYLREISKYTRWEYEFVKGSWEECLDRLEKGEIDLLGPLQ
                     KNDERNKLFNFPKLSSGYEYSALYTKDSNNNMFYEDISSFKGMRVAVLRGNFHNTAFE
                     KYREENNFSVEYIYCNSIDELIESVNEKKADAFVCGSIINAKGMKIVSKFSVEPFYFA
                     TAKDKPNLVKELDYALKELKINDMYYELELYKKYFKREVNNSIAFTRQEMNFIKANPK
                     LTMVYDSEWSPVEYYDKESNSFKGISSDLMSIISKKCGIKFEYIKTKNYNESLDYIKS
                     GKATMLCGSINENDKAKRFNMKLTNPYINIPMIMVGKLDTNLNNDLNIALTSSYKSID
                     SYIEKSFENAKTTSYKSVESCLNAINEGKANLMILSSYQFDELLREGKSENLSVISVL
                     DTSYGMRIGVSNKTDPILVSILNKSIDKITEEELSDCIYSNTIDKPYKVPFGVIFKEY
                     SIQIISFVCILFLIAIKYIIYNKKKKEDYLRKIAYTDPLTGADSIDKFKINSNKLFAK
                     NNPEEYALFYIDVDKFKYINDMFGYDMGNDTLIHISNTIASELKEDEIFARVSADHFV
                     LLIKYKTDDDIKTRLNNIYNKVQILSNPKINYYKLILDCGIYKISKSDNDINTIMDRA
                     NTARKTIKGGHKNSFAFYDKEMHKKILKEKEIENSMVDALNNGEFIVYFQPKYSLSDY
                     QIIGAEALVRWDNPQKGLIPPIEFIPVFERNGFIVNIDFYVFEEVCKKIREWMDEGQK
                     VVPISVNLSRMHFVNSNFIEKFKLIVDKYKIPTRLIELELTETAVLDNIEGLLDTMNN
                     LKEKGFVISMDDFGTGYSSLNLLKELPVDILKLDRAFFTEKDESNNEKIVISNVIKMA
                     KELKMKVISEGVETISQVEFLKQIGCDMVQGYLFSKPMPVKEFEKIAFKKE"
     gene            4843..5055
                     /locus_tag="MJE33_003204"
     CDS             4843..5055
                     /locus_tag="MJE33_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBY9649120.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            5068..5289
                     /locus_tag="MJE33_003205"
     CDS             5068..5289
                     /locus_tag="MJE33_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBY9649121.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEVTVRDYELSIRKSDAEKIIVE"
     gene            5389..7545
                     /gene="feoB"
                     /locus_tag="MJE33_003206"
     CDS             5389..7545
                     /gene="feoB"
                     /locus_tag="MJE33_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBY9649122.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTVIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDESKDAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKTILNSIEEITRNCEDELDDDSESIITANRYEFISSIISSIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKIPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHIPSAKGVLIHM
                     WDRGKAFIIKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIVAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVASAFAFMAF
                     NMLCAPCFAAIGAIKREMGSWKWTWITLGFQTLTAYIIALLINQVGSLVLGTGGSIAG
                     AIISIFIAVAVVFVVLTYSNKNMKKEKMGKLSYMKN"
     gene            7571..7723
                     /locus_tag="MJE33_003207"
     CDS             7571..7723
                     /locus_tag="MJE33_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBY9649123.1"
                     /translation="MATFIIAAIVVVLMALAVMYMVKNSKKNGSSCGCGCSGCSSSKS
                     CHSAKR"
     gene            7903..8613
                     /locus_tag="MJE33_003208"
     CDS             7903..8613
                     /locus_tag="MJE33_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBY9649124.1"
                     /translation="MFKFDVTNELMCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPS
                     NGGGLGRALLGHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESE
                     NLLAFSEQLNYSVKLIGSGVISQKGLFTTHLVNKTGQNQDVAIITDGNPLILEGPCCV
                     DPDALVAWTGREPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKT
                     DSTPSDRMSSGGLRLGID"
     gene            8698..9468
                     /locus_tag="MJE33_003209"
     CDS             8698..9468
                     /locus_tag="MJE33_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9649125.1"
                     /translation="MFEKIHKRLKGYYIFLIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVLFGANKKMYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDNKLIEVGKSFNL
                     SKKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            9461..10192
                     /locus_tag="MJE33_003210"
     CDS             9461..10192
                     /locus_tag="MJE33_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9649126.1"
                     /translation="MVRSGFLLENIYKKYLVDNKEHLVLDNISLNISSEEITVILGES
                     GCGKTTLLRILAGLENATSGNVYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVNIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            10226..11185
                     /locus_tag="MJE33_003211"
     CDS             10226..11185
                     /locus_tag="MJE33_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBY9649127.1"
                     /translation="MTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPKEINLTYVKSPL
                     NVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFLHALGGTSAIIA
                     ASNGVGLKITNIYSRSPKGYMLITNNDNIKSIKDLKGKKIVGPKGTILHQLLIAALAK
                     EGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKMIVNGEGLVSGI
                     IVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDEVMDVVSKEVGLSLDETKEMYS
                     WYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(11283..11879)
                     /locus_tag="MJE33_003212"
     CDS             complement(11283..11879)
                     /locus_tag="MJE33_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HBY9649128.1"
                     /translation="MSCCNSQFCCKNKIESSYIKWLLEVLGPVFLGSKPAEIINISFT
                     DVNREEKINDIYKYLYKCKKIDFIVIDKEKKGLKILFVNKKALSEKLKCKKTVNFLKF
                     LGYKQNTNVNAYLEHLVDKLKSDVFPDEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWK
                     VYGDTKQSEDTYSKFLLHRKKMRKLLDYISVDKIVSCF"
BASE COUNT         4582 a         1219 c         2157 g         3961 t
ORIGIN      
        1 aaattgggct gatgagcaca gaataggagt agctcaaggt gtagatgaag aaataatcga
       61 aggattaaga gctaactttg ttggtgagtg tacagaagta ggaatgtatt tagcaatgag
      121 tagacaagct gatagagaag gttatccaga agtaggagaa gcttataaga gaatagctct
      181 tgaagaagct gaacatgctg ctaaatttgc agaacttctt ggagaagttg tagttgcaga
      241 tacaaaagaa aacttaagag ttagagttga tgctgagtat ggtgcaactg atggaaaatt
      301 aaaattagct aagaaagcaa aagaattagg attagatgct atacatgata cagtacatga
      361 aatgtgtaaa gatgaagcta gacatggtaa agcattctta ggattattaa atagatattt
      421 tggaaaataa atttaatata aagtagattt taggagtatc tcacttataa tttttaaagt
      481 tatgagtgag atatttttta attatttgta ctttattttt tatttctaac gattatattt
      541 ttaataatag agaataattt aatagtaata atgcacaact tttaaaaata aatgattaat
      601 ccaaaaataa atttgatttt agaaaagtaa attgacatgt aaagaagaat atattaaact
      661 aagtataaaa aatttgctgt tttgtaaagg tggtggataa tctgataaag tatatacata
      721 aaagtgatgt agctattgtt gttttgcatg aaatctatgg gattaatagc tttatagaag
      781 atgtttgtca aaagtattat aaatatggat atgatgtatt ttgtcctgaa cttctgggag
      841 aaaaaagagt ttttccatat tcaaaagtcc aagaggcata tgattttttt ataaataatg
      901 ttggttttga tatttataaa aaagttgaaa agcttataaa tgatttaaaa tcagagtata
      961 aatatgtatt tgttgctggg tatagcgtgg gagcaactat tgcatggaga tgcagtgaag
     1021 attctttttg tgatggcata atttgttgtt atggctctcg gattcgagat tacatggatg
     1081 taattccaaa atgtcctacc ttattagtat ttgcaaagca cgattcattt gatgttgatt
     1141 ctatttcttg ccaactttct aggaaacaaa atatccaaat tgagatttta gatgcaaatc
     1201 atggttttat agatagatat tcaaataatt attctgatat tcaaacaaaa gtatttacta
     1261 ttaaagaaaa aagttttctg tctaaatatt tagcatgatt tagactatta taaaatatat
     1321 agaaagattt aattaatctg aaaagttttt atgacactat tgatatttaa aatattagta
     1381 gtgttattat attttttgaa ataaaattaa tcactagaat tatagtgtta gttgtgcttt
     1441 tatgacttat aagaaactaa tattagatag tgtatttaaa tagataataa taaattcatt
     1501 atagttgaat aagaatatta tatgagaata ttgcaatttg atatatttac cattacaata
     1561 aaagtagtta caactagaaa accaaataag ttaaagtaag ttggcaaatt ggaggttttt
     1621 aaatatgaaa gcatttaaaa agcacttctt gatactttct ttgatatttt tacttattat
     1681 atcatgtttt aacaatatac tatgctttgc tgatgagtct gaaaattcaa aaaaaataag
     1741 agttggatat tgtgatgact acggaataat atcctcatca aaagagcatt cttatacagg
     1801 atatggttat gattatctaa gagaaatctc aaaatataca agatgggaat atgaatttgt
     1861 aaaagggtct tgggaagaat gtttagacag actagaaaag ggtgaaatag acttactcgg
     1921 acctttacag aagaatgatg aaagaaataa actatttaat tttcctaaac taagttcagg
     1981 atatgaatat agtgcactat atacaaagga tagtaacaat aatatgtttt atgaagatat
     2041 aagttccttt aagggaatga gagtagctgt acttagagga aactttcata atactgcatt
     2101 tgagaagtat agagaagaaa ataatttttc agttgaatat atatactgta attctattga
     2161 tgaattaatt gagtccgtca atgaaaaaaa ggcagatgca tttgtttgtg gaagtattat
     2221 aaatgctaaa ggaatgaaaa tagttagcaa attttcagta gaaccttttt attttgctac
     2281 tgcaaaagat aaacctaatt tagtaaaaga attagattat gctttaaaag aattaaaaat
     2341 aaatgatatg tattatgagt tggaattata taaaaaatat ttcaaaagag aggtcaataa
     2401 ttccatagca tttactcggc aagaaatgaa ttttattaaa gcaaacccaa aacttactat
     2461 ggtatatgat tcagagtggt ctcctgtaga gtattatgat aaagaaagca attcatttaa
     2521 aggtataagt tccgatttaa tgtcaattat atcaaaaaaa tgtggtataa aatttgaata
     2581 tattaaaaca aaaaattata atgagagtct tgattacata aaaagtggta aggcaactat
     2641 gctatgtggc tcaatcaatg aaaatgataa ggcaaaacga tttaatatga aattgacaaa
     2701 tccatacata aatattccaa tgattatggt aggaaaattg gataccaatc tcaataatga
     2761 tttaaatatt gctttaacta gtagttataa gtctattgat tcttatatag aaaagagttt
     2821 tgaaaatgct aagacaacat cttataagag tgtagaaagt tgtctaaacg ctataaatga
     2881 aggtaaagca aacttaatga tacttagctc ttatcagttt gatgaacttt taagagaagg
     2941 aaagagtgag aatttatctg ttatatctgt tttagataca agctatggga tgcgtatagg
     3001 ggtatcaaat aaaactgacc ctatattagt gtctatttta aataaatcaa tagacaaaat
     3061 tactgaagaa gaattaagtg attgtatata ttctaatact attgacaaac cttataaagt
     3121 accatttggt gtgattttta aagaatattc aatacaaatt atttcttttg tatgtatttt
     3181 attcttaatt gctattaaat atataattta taataaaaag aagaaagagg attatctaag
     3241 gaagatagca tatacagacc cattgacagg agcagatagt atagataaat ttaaaataaa
     3301 ttctaataaa ctgtttgcta aaaataatcc agaagaatat gcgttgtttt atatagatgt
     3361 tgacaaattt aaatatataa atgatatgtt tggatatgat atgggaaatg atactttgat
     3421 acatatatca aatactattg caagtgaatt aaaggaagat gaaatttttg caagagtttc
     3481 tgctgaccat tttgttcttc ttattaagta taagacagat gatgatatta aaacaaggtt
     3541 aaacaatatt tacaataagg ttcaaatact tagtaatcct aaaataaact attataaatt
     3601 gattttagat tgtggtatat ataaaataag taaaagtgat aatgacataa atactattat
     3661 ggatagagct aatactgcaa gaaaaactat aaaaggtggt cataaaaatt catttgcttt
     3721 ttacgataag gaaatgcaca aaaaaatatt aaaagagaaa gaaattgaaa attcaatggt
     3781 agatgctctt aataatggag aatttattgt ttattttcaa cctaaatata gtctttcaga
     3841 ttatcaaatt ataggggcag aggctcttgt tcgttgggat aatccacaaa aaggtctaat
     3901 accaccaata gaatttattc ctgtatttga gaggaatggt tttattgtaa atattgattt
     3961 ctatgtattt gaggaagtgt gtaaaaagat tagagaatgg atggatgaag gacaaaaagt
     4021 agtacctata tctgtaaact tgtctcgaat gcattttgta aatagtaatt ttatagagaa
     4081 gtttaaatta attgtagata aatacaaaat tccgacaaga cttatagaat tagagctaac
     4141 agaaacagca gtattagata atatagaagg attacttgat acaatgaaca atttgaaaga
     4201 aaaggggttt gtaatctcta tggacgattt tggtacagga tattcctctc ttaatctttt
     4261 aaaagaattg cctgttgata tattaaaact agatagagca ttttttacag aaaaagatga
     4321 gagtaataat gaaaaaattg ttatatctaa tgtcattaaa atggcaaagg aactgaaaat
     4381 gaaagtaata tcagaaggtg tagagactat ttcgcaagta gaatttctaa agcagattgg
     4441 gtgtgatatg gtacaaggat atttgttttc taagccaatg cctgtaaaag agtttgaaaa
     4501 aatcgctttt aaaaaagaat aaagcaatgt gtaaataatg acatatataa taaaggtgct
     4561 ttaatattag ttgagtatac tattttagag catctttatt tttgtattaa tttttacgat
     4621 tttattaata aaataaatca aatgagaaaa aaaatcaaaa gaatacaata atcacttgca
     4681 aaaaatatac tattgtgata gacttaaatc aaaattagga attgtaaaaa gaaggaaaaa
     4741 ataggatatt attttaatag attaagattt aaggtattgt aatttatata taagataacg
     4801 attgcttgta aatttaaatt actttaagag gagggatatt atatgatgcc attgatatta
     4861 tcaaatacag gtgaagaagt agttataaag aaaatttcgg gaagtgataa gacgaggact
     4921 tttttaaata gtataggttt tgtagtgggt acaaatgtta aaatagtttc taagatagat
     4981 ggtaacttaa taatagacgt aaaagatact agagttgcat tagataaaaa aatggcaagt
     5041 agaataataa tatagggagg taatgacatg aacagactga aggatattaa gtgtggagag
     5101 actgtaaaag tcaaaaagtt agaaggagaa ggagctacac gaagacgtat aatggatatg
     5161 gggattacaa gaggtgttaa tatatttata agaaaagtag ctcctcttgg tgacccaatt
     5221 gaggtaacag tcagagatta tgaactatca attcgtaagt cagatgcaga aaaaattatt
     5281 gtggaataat aaagattaat tagagtaggg ttaatgtaat catataatga gaatgatttt
     5341 ctgtatcaca aacagtgatg tatttaaata ataatggagg gaatggaaat gtcaataaaa
     5401 ataggtctca taggaaatcc taactgtggt aagacaacaa tgttcaatgg gcttacaggc
     5461 tcatcacaat atgttggtaa ctggccagga gtaacagttg aaaaaaaagg tggaaaatta
     5521 aaaggaaata aagatgtgga aatagtagat ttaccaggta tttattcttt atctccatat
     5581 acattagaag aagtagttac acgtaatttt atgctagatg ataagccgga tgcagtaata
     5641 aatattgtag atgcatcaaa tatagagaga aatttatatc ttacaactca agttttagag
     5701 ctaggtatac cgaccgttat agctcttaat atgatggata ttgtaaataa gaatggtgat
     5761 aagataaata ttaaagaact atctgaagtt ataggttgcc cagtggtaga agtaacagca
     5821 gttaaaggtc aaggaattat ggaagctgct gaaaaggcag ttgaacttgc aagtagtaac
     5881 aataaattaa atttcaaatt accctttgtt gatgaaagta aggatgcaat agaaaaaatt
     5941 gaaaaaataa tagaagaaaa gactccatat atagatgtag aaacacgctg gttagctata
     6001 aagctatttg agagagatga aaatgttata caaaaacttg atatttctaa aacaatattg
     6061 aatagtattg aagaaataac tagaaattgt gaagatgaat tggatgatga tagtgaaagt
     6121 ataataacag ccaatcgtta tgaatttatt agttcaataa tttcaagtat aataaagaag
     6181 aatcgaaaag gtaaggaaac tgtatcagat aagatagata agattgtaac aaatcgtata
     6241 ctagcattac ctatttttgc acttataatg tggggagttt actatatagc agtaagttca
     6301 ttgggtacta tagcaactga ttggacaaat gatgtactgt ttggagaaat tattcaaggc
     6361 aatgtaagta attttctagc ttctttaaat gtagcagagt ggttacaagg acttgtagtt
     6421 gatggtctaa ttggtggagt tggagcagta ctaggatttg taccacaaat catgctttta
     6481 ttcttattat tatcaatatt agaagattgt gggtacatgt cacgtgtagc ttttataatg
     6541 gatagaattt tccgtaagtt tggtctttca ggaaaatcat ttataccaat gcttataagt
     6601 tcaggttgtg gagttccagg agttatgtca actcgtacta tagagaatga tagagataga
     6661 aaaatgacta ttatgttaac tacattcatt ccatgtggag ctaagattcc aataatagca
     6721 ttatttgcag gggcattatt tggaggtgct tcatgggtag ctccatcaat gtatttctta
     6781 ggtattgcaa tgataatcat ttgtggaatt atattaaaga aaactagttt atttgcagga
     6841 gaaccatctc catttgttat ggaattacct caatatcata ttccaagtgc aaaaggtgtt
     6901 ttaatacata tgtgggatag aggaaaagca tttataatca aagcaggaac aattatattt
     6961 gttgcttgtg gagtaatctg gttcttacaa tcatttaatt ggtcattaca aatggtagat
     7021 gctggagata gtatattagc tagcttagga aatatagttg caccaatatt tgctcctctt
     7081 ggatttggaa attggcaatc atcagtagca acagttacag gtctagtagc taaggaaaat
     7141 gtagttggaa catttggagt tttatttggt atatcagatg caacagagca agacccaaca
     7201 cttcttgctt cagttgcaag tatgtttaca gtagctagtg cgtttgcatt tatggcattt
     7261 aatatgcttt gtgcaccttg ttttgcagct attggagcca taaaaagaga aatgggttca
     7321 tggaaatgga catggattac attaggattc caaactttaa cagcgtatat aattgctctt
     7381 cttataaatc aagttggaag tttagtttta ggtactggag gaagtattgc tggggcaata
     7441 atatctatat ttatagcagt agcagtagtc tttgtagttt taacttattc aaataaaaat
     7501 atgaaaaaag aaaaaatggg taaattaagt tatatgaaaa attaattcat ataaataagg
     7561 agtgatgaat atggctacat ttattatagc agctattgta gtagtattaa tggcactagc
     7621 tgtaatgtat atggttaaaa attcaaaaaa gaatggtagc agttgtggat gtggttgttc
     7681 tggatgctca agtagtaaga gttgtcatag cgctaagaga taaataggat aagagcctga
     7741 taaaaagtta aattgcttta ttatcaggtt cttatttttt gtataaataa caaatttcat
     7801 atttgaagta ggttagacta tgctgtaatt ttactgtata attatatata aatatatgat
     7861 taggagggat tattgctaaa ttaagcaata taagtgaata ttatgtttaa atttgatgtt
     7921 acaaacgagc ttatgtgtat agcaagggga agtggtaaat tttttgcaaa aaaaggtgct
     7981 atggtagcct ttaaaggaaa ttttaatttt gaaaaattgc tgttaggtcc aagtaatggc
     8041 ggtggattgg gaagagcatt attaggtcat gttcagaggt cattgacagg agaacaaatg
     8101 ccaataatgg tggttgaagg tgaaggtgaa atttacctag ctcaaaatgc ttaccatgta
     8161 gatgtaatat caattgaccc aggtgattcc atttatgttg agagtgaaaa cttattggca
     8221 ttttctgaac aattaaatta cagtgtaaaa ttgattggtt caggggtaat atctcaaaaa
     8281 ggtcttttta ctacacattt agtaaataaa acaggtcaaa atcaagatgt tgctataata
     8341 acagatggta atcctctaat tcttgaagga ccttgttgtg ttgacccaga tgcattagta
     8401 gcttggactg gaagagaacc atatcctaaa gtatcaaaac tatcatggaa gacttttatt
     8461 ggtcagactt caggagaatc ttatcatatg gaatttgttg aacctggaca aatagtcatt
     8521 atacaaccat ctgaaagatt gtctggctta aaaacagatt ctacaccttc tgatagaatg
     8581 agttcaggtg gattaagact tggcatagat taaatcttat gatatagctt aattattatt
     8641 taagatatta aaaaataaaa agggtagcag ggatagatat gttaaaagga gttttatatg
     8701 tttgagaaaa ttcataaaag gcttaaagga tattatattt ttttaataat tttaatttta
     8761 tggaagatag tatgtgtttt gggtatatgg agtagctata ttctgcctcc tcctgaaata
     8821 gtcttagata cttttataaa aatgatatat gacggttcta ttttcttaaa tgtttatgcc
     8881 agtgtaagaa ggatattaat aggtttttta ataagttcat tgatggctat accacttgga
     8941 gttttatttg gagcaaataa aaaaatgtat gaatatttca aaccactatt tgaatttatg
     9001 cgaagtacac ctccactagc tttggtgcca atgcttattt tatggtttgg tataggtgaa
     9061 gaatctaaaa ttataattat tattttggca tccttttttc caatattttt aaatacattg
     9121 aaaggtataa aaaattgtga caataaatta atagaagtag gtaaatcttt taatttatct
     9181 aaaaagagaa ttttttataa aataataatc cctaattcta tgctggatat tgttgttgga
     9241 cttagtttag ggcttgggta tagttttagg gctataattg gagcagagct tattgcagct
     9301 tcttcaggac taggatattt gatttctgat ggaaaagata tgtctagaac tgatgttgta
     9361 ttggtaggaa tttttgttat tggattttta ggtatacttt cagattatct attttcaaag
     9421 ataataacaa aatttagcaa agggaagcag gttgatttaa atggttagaa gtggattttt
     9481 gctagagaat atatataaga aatatttagt tgataataaa gagcatttag ttcttgataa
     9541 tatatcttta aatatatcta gtgaagagat aactgttata ttaggtgaaa gtggatgtgg
     9601 gaaaacaacg ctacttagaa ttttagcagg gcttgaaaat gctactagtg gaaacgtata
     9661 tttttttaat aatgataaaa agtgtactcc aaaagtagga atggtatttc aagaaagtag
     9721 acttatgcca tggctgaatg taagtgaaaa tatactttta catactgaaa aagataatag
     9781 aaataaagtt gatttagaca agtatttaaa aatgatgaag cttgaaaagt ttaaaaactc
     9841 atatccaaat gaattatcag gaggaatggc tcacagagta agtatagcaa gagcactttc
     9901 cttcaatccc gatattttgc tgatggacga accatttgca gcactagatt acttcacaag
     9961 aagaaaaatg caaaaagaag ttgtaaatat ccataaaaat actaaaaaag gtgtagtatt
    10021 tgtaactcac aatattgaag aagctatgga aatagcaaag aaaataattg tttttagtaa
    10081 aaataaaagg attaaacaat ttagtgtcga agatgaatat aatagagatt taactaaaaa
    10141 ctattatata aacttgaaaa aagaaatatt aagggaattg ggggagtttt aattgattaa
    10201 aagaaacaaa aagattttag cagtaatgac atctttaata atggtgttgg ttgggactgt
    10261 aggatgttca acaacaaatg ataagaaaga tgaacctgca aaaaaagagg ctagtttacc
    10321 taaggaaata aatttaactt atgtaaaatc accacttaat gttcctagta tattagaaaa
    10381 aaaggatgat ttatttggaa aggaatttaa aaaggacaat ataaaagtca atttccatga
    10441 attgacaaca ggaccagagc aaactcaagc attagcagca ggtgagctag actttttaca
    10501 tgcattagga ggaacgtctg caataatagc tgcatcaaat ggtgttggtc ttaaaattac
    10561 gaatatttat agccgttcac caaaaggata tatgcttata actaataatg ataatattaa
    10621 atctataaaa gacttaaaag gtaaaaaaat tgtaggtcca aaagggacta tacttcatca
    10681 actgttgatt gctgcacttg ctaaagaagg ctatactata gatgatgttg aatatgtaaa
    10741 tatggatatt cctagtgctg cttctgcatt agaaagtaaa agtgctgatg tagcaatgct
    10801 tgcaggtcca gtggcattga aaacgataaa ttcaggtgcc aaaatgattg taaatggaga
    10861 aggattagta agtggaataa tagttactgc tgtaagtgat gactttgctg aaaaatatcc
    10921 tgaattagta aaaagattta tgaaggttca tgaagaaact ttaaaatata tgaatgaaaa
    10981 caaggatgaa gtgatggatg ttgtatctaa agaagttggt ttatctttag atgaaacaaa
    11041 agaaatgtat tcttggtatg actttagttc taaaataacg gacaaggata taaaagaatt
    11101 agaagataca caggaatttt tgatgtctaa tggaatgcaa caaaagaaaa taaatattaa
    11161 agatatgttg tacaatcaaa attagaatac tttaaaatat taaagtggaa aatacaccct
    11221 tatttattat ggaaactgta tggtaaaaga gggtgtattt ttattattat acaaaaacac
    11281 aattaaaaac agcttactat tttatctaca cttatgtagt ctagtaattt tctcattttc
    11341 ttacggtgaa gtaaaaactt cgaatatgtg tcctcggatt gttttgtgtc accataaact
    11401 ttccaatatt taatcataga aacttcataa ttactatatc ccataaatcc aatcacatct
    11461 ttaagaggat atcctaaaaa aatacctatt tcatcaggaa aaacatcact cttaagttta
    11521 tctactaaat gctctaaata agcattaaca ttcgtatttt gtttgtatcc taaaaacttt
    11581 aaaaagttta ctgttttttt acattttaat ttttctgata atgctttttt atttacaaat
    11641 aagattttta atcccttttt ttctttatca ataactataa aatctatttt cttacactta
    11701 tatagatatt tataaatatc attaattttt tcctctctat ttacatcagt aaacgaaata
    11761 tttattattt cagcaggttt agagcctaaa aagactggac ctaaaacttc taaaagccat
    11821 tttatatatg atgattcaat tttattttta caacaaaatt gtgaattaca acaagacata
    11881 aaagataacc tccttcaaat gaaaattaat atcaagtaa
//
LOCUS       DAGSRM010000113        11822 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.114, whole genome shotgun sequence.
ACCESSION   DAGSRM010000113 DAGSRM010000000
VERSION     DAGSRM010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11822
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.114"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            184..1224
                     /locus_tag="MJE33_003213"
     CDS             184..1224
                     /locus_tag="MJE33_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY9649129.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFTRIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKILE
                     YFKLNNINIKLLEESDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
     gene            1552..2898
                     /locus_tag="MJE33_003214"
     CDS             1552..2898
                     /locus_tag="MJE33_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBY9649130.1"
                     /translation="MSKYQKNVSMFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVV
                     GGAVGVSPTQITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVG
                     GNYGLASILGAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMA
                     GGVNSPTYASMQNWAVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIIN
                     FDPIREATWFAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNIT
                     DKELSSGVIGSGICTMIGALFGGLPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSGF
                     VPKFGALMTTIPYSVLGGATISIFSMITMTGIKLIITEELSSRNITIVGLAIALGMGI
                     TSVPAAQEAFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
     gene            3195..4556
                     /locus_tag="MJE33_003215"
     CDS             3195..4556
                     /locus_tag="MJE33_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBY9649131.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSITSIISSLFAALPTGVFSQNTVIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            4629..6023
                     /locus_tag="MJE33_003216"
     CDS             4629..6023
                     /locus_tag="MJE33_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBY9649132.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLDAKKVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMVELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNVILENGKVTTIESEEQVLLNAQHAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            6085..6555
                     /locus_tag="MJE33_003217"
     CDS             6085..6555
                     /locus_tag="MJE33_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HBY9649133.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLAKNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIREAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            6558..8774
                     /locus_tag="MJE33_003218"
     CDS             6558..8774
                     /locus_tag="MJE33_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBY9649134.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSIDTSEAEKVVGVRAIATYLNTSQRKYNGSLRFYEHKIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAVKLIKVEYEELPAVFTIQDAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKEIGMDLVDFQNKNAVEPD
                     DVDIIFHGSLGNPRVLDCIEQGKKMFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLLTGAHDMGNGVVTMQTMMVAEVLGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKNLILEEAEKLLETSKEDLYLDNGCVISKL
                     DENVKASLSDVAVYSQSKSLRELTVVEDYSSPAGRTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVDGYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            8857..9528
                     /locus_tag="MJE33_003219"
     CDS             8857..9528
                     /locus_tag="MJE33_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBY9649135.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
     gene            9891..11105
                     /gene="dpaL"
                     /locus_tag="MJE33_003220"
     CDS             9891..11105
                     /gene="dpaL"
                     /locus_tag="MJE33_003220"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBY9649136.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAQEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNHAAAFVSAPDWVSAKGMRTLGNP
                     LNGDEKVISGESGAVTTGLLVAAMEREDLADLRKDLKLDENSRILLISTEGDTDPDKY
                     RSIVWDGEYPSI"
     gene            11232..>11822
                     /locus_tag="MJE33_003221"
     CDS             11232..>11822
                     /locus_tag="MJE33_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBY9649137.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNRDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKG"
BASE COUNT         4470 a         1359 c         2399 g         3594 t
ORIGIN      
        1 agagcatttt tgtaaaatga agaaagtata tctatcaaat taaaactaag tagatatact
       61 ttttattaag ttttgtttta aaataatatt attaaattat aactaagctt ttctgttatg
      121 atataattaa atagataatg tgaatgatta ttatatagaa tacttatttg gagttgatat
      181 tttatggaca aacacagtga ttcagtaatg tatggtggaa tggaagtata taatatttgg
      241 ggaacaacaa cagtagactc cataattgga gagtctgatg taatgcgtgc tcttaagaat
      301 agaatttgta aaattggaaa tagtgattct actgtagcta taactggaga aagtggcact
      361 ggaaaagaat taatagcaag agccattcat ctcgcaggaa acagagggaa taaaacattc
      421 gttgctataa actgtgctgc tataccagaa cctttactag aaagtgaact ttttggatat
      481 acaaaaggag cttttagtgg agcagattca aaaggtaaaa ttggtaagtt tgaattggcc
      541 aatggagggg ttgtattttt agatgagata ggagatatgc caattcaact acaatcaaag
      601 ttattgaggg tattacagga aaagaagttt acgagaatag gttcaaatga actaattgat
      661 atagatgtta gaatcatatc agctactaat agagatttat ttgagttggt taaggaaaat
      721 aaatttagag aagacttata ctatagattg aatgtaatac ctttagaagt gcctccactt
      781 agagaaagag gagacgatat acaaatatta ataaacaatt ttatagacaa atattcgaga
      841 aagatgaaga aaaacgtgta ccatatagag ccagatgtgg aacaaatgtt gcttaagtat
      901 ccttggccag gaaatattag agagttggaa aatactatag aactggcatt aaaccttctt
      961 gaggaagatg gaataataca taaaggaata ataaatagaa agatacttga atattttaag
     1021 ttaaataata taaatataaa gttacttgag gaaagtgact atgagataag tatggaacaa
     1081 gacatactta cacttgaaga gtttgaaaga gcctatataa agaaagtgtt aaagttttac
     1141 ggaaatgata ctaagtctaa aaaactggct gccaaaaaac ttggaatatc actagctaca
     1201 ttgtacagaa aaatagatgt ataataatta ggctttttat tctcaattct aaaatttgaa
     1261 attataaaat tgataaaaaa cacttggaaa actattttct aagtgttttt ttgtgtaata
     1321 gcattttatt atccaaaaat aaaaaaaatt taaaagaatt taaaaaaata aatgctttaa
     1381 taaattatca aatactaaca aatataataa aaattatata tgtaaaacaa ttatgttaaa
     1441 aaattggcat tataattgct tcatattaag ttgagatgac aaaataaaaa aagaaaggat
     1501 gttgtgtgag ataataataa ctgtcattaa aaatagttgg gagtgataac cttgagtaaa
     1561 tatcaaaaga atgtttctat gtttgatatt gatggaaggc caccaatttt aaagtgtttg
     1621 ccactatcaa tgcaacatat actagcaatg atagttggaa ctgttacagt acctatagtc
     1681 gtaggaggag ctgtgggtgt tagccctaca caaataacac ttttagtgca atatgcatta
     1741 ataatagctg gtatatcaac acttatacaa ctatatccaa taggaaatat tggttcaaga
     1801 ttgcctataa tttttggagt tggttttact tatgtaccaa cacttgtagc agtcggagga
     1861 aattatggat tagcaagcat tttgggtgca caattaatag ggggagtggc tacagtatta
     1921 atagggatat tcataaaaaa gataagaaaa ttttttccga atatagttgc aggaacagtg
     1981 gtttttacaa ttggattatc tttgtatcct atagcaataa attatatggc aggtggtgta
     2041 aatagtccta cttatgcctc tatgcaaaac tgggcagtag cggctataac attagtggta
     2101 gttataggtc ttagtcaatt tgcaaaagga tatgcaaaat tggcagcaat atttttggga
     2161 attatagttg gatattgtgt atctttagtt ctgggtataa taaattttga ccctattaga
     2221 gaggcaactt ggtttgcgat acctaaaccg cttgaatttg ggatggaatt tgatatgcct
     2281 gtaataatct cagtagtaat tgtaagtata atcaatgctg ttcaagcaat tggagactta
     2341 tcagctacta caatgggagg catggatagg aatatcacag ataaggaatt atcaagtgga
     2401 gtaataggaa gtggaatttg tactatgata ggagctttgt ttggaggttt accatcatca
     2461 tatagccaaa acgtggggat ggtagctatg aacaaagtaa taagtagatt tgtgcttgga
     2521 atagctggta tatttatgtt attgtcgggg tttgtgccta aatttggtgc attaatgact
     2581 actataccat attctgtttt aggaggagct acaatatcta tatttagtat gataacaatg
     2641 acaggtataa agttaattat tacagaagag ttgtcatcaa gaaatattac cattgttggt
     2701 ttggcaattg ccttaggcat gggaataaca tcagtcccag cagcccaaga ggcatttcct
     2761 tcatttgtaa agttagtatt tggagaatct ccaatagtaa ttgcaacatt ggttgcattc
     2821 gttttaaatc taataatacc aaataaaagt ctagaggatg aggctaaaga aagagctgtt
     2881 atggaagaga ctaattaaga tataataatt taaaagaatt gtttaaatta tttagtatga
     2941 gattgttaga ttatttttga tagctattca ttttaataaa aaatttaaca aataaatata
     3001 taccaggcaa ttaatgttca tttaagttga aaatcgggga ggcaatattt agaaatttgt
     3061 taatttttta taaaaatagc tcagtttagc tgtgtttatc ggttgagcaa taaattcatg
     3121 gtcataaaaa tatttataga aaataattat aaaaatattt agtcaagata tttatgtatg
     3181 ggaggattta ttttatgaaa acacgtaata cttcaaaata tgatgtcgat gggataccac
     3241 cactaaagga atcaatacct ttggccttac aacatctgtt agcaatgata gtaggaaata
     3301 tggtacctgc gatattaata gcaaacgtgg taggacttaa ccaggggcaa gcaacaatgt
     3361 tgattcaggg ttctatgtta gcagcaggtt tagcaacgtt tttacagtta tatccaatac
     3421 ctttatttaa aggatttaag ttaggttcaa gattacctgt aatgatggga atgagttatg
     3481 tatttttagg agcttgtctt tcagtagcag ctaaagatgg tttagcagct ctatttggag
     3541 cacagattgc tgctggggta atcgtattct ttgtaggatt tggagttaag aaaattagac
     3601 atatattcac tccaatagtt tctggaacta taatagcatg tatgggactt ggactattcg
     3661 caacagctat aaagaattta gctggaggag aaggcacaca aacatttgga agcccaataa
     3721 acttcacagt aggagttata gttgcttttg taataataat gataaataaa tacggtaaag
     3781 gtttagtaaa aaattcttca atattaatag gaatattagt aggttatgct atatctttag
     3841 ttttaggatt ggttgatttc tcagccgttc aaggagcagc tatagtatct ttaccaacac
     3901 cagcagcttt tggattagaa tttagaccag agttaatagt aatgtttact attatttaca
     3961 taataggtat agctgatatg atgggtgcct gtacaatagc tacagtagga gcaatggata
     4021 gagaggtaac tgacgaagaa ttagcatcag ttgtattagg aaattctata acttctataa
     4081 tttcatcact atttgcagct cttccaactg gagtatttag ccaaaataca gtaattgtat
     4141 ctatgaacaa agttacaagt agatttgtaa tagcactagg ggcacttgta ttattactag
     4201 cagggatatc tccagcattg ggtgcaataa tgacaactat accatcttgt gttgttggtg
     4261 gagcaacttt agtagttttc tcatcaatag caatgtctgg tttctctatt atgagtatgg
     4321 atggattcac tgaagaaaac aatcttatag caggtgtatc tatagcaaca agtatgggtc
     4381 ttacaactgc tccacaagtt ttagaccaat tcccagaaac aattggaact gtccttggag
     4441 ggtcaagtat agtttctgga gcaataatag ctatattact tcaaacatta tttaaattaa
     4501 agtcacgtaa aagtgctgaa aacgttactt caaatcttga ggaaaatatt ggataaatgt
     4561 attagatata atatgagaaa accatatggt aaaatgttgt aatgaaagta ttcaaagggg
     4621 gatttaagat gattgatata ctaataaaaa atgcaataat tgttacagtt aataaagaca
     4681 gagatgttat atttgatgga gcaatagcta ttcaaggtga cagaatcttg gacataggaa
     4741 atacagttga tattgagcct aagtatcttg atgctaaaaa agttatagat gctaatggaa
     4801 aagtaatatt ccctggcttt ataaatactc ataatcacct gttccaagtg ttattaaaag
     4861 gtttaggtga tgatatggca cttcatgagt ggttaaatac aatgatgttt ccatcagcaa
     4921 aattcttaac agaacaagat acttatgatg ctgcaatgct tggatgtatg gaaggtttaa
     4981 aaagtggtat aactacaatg gttgattata tgcatactca caatagacca ggacttacag
     5041 atggaatagt aaaggcatat aaagatttag gtataagagg ggttgtaggt agaggttgta
     5101 tagatttagg tattcacaaa gagcttatag aagatgtgga aacggttgaa aaagatttga
     5161 gaagggtatt cgaaaaatat cataattcag aaaatggaag aataaaaatc tgcgtagctc
     5221 cttcttcaat gtgggctata tctgaagaaa tgggtaagat gctttggaat atagttaaag
     5281 aatatgattc atattttaca gttcatatat ctgaaacaga atttgcaaga acagcaacaa
     5341 aggatataca tggagaaata gatataaaat tacttgaaaa atggggtata gtaggtccag
     5401 aagttgtagc agtacattgt gtatgtataa ctgatgaaga catggaaatg cttaaaaagt
     5461 atgacataaa agtttctcat aatgtagcaa gtaacatgta tcttgcttct ggtgttgcac
     5521 ctgtgccaga aatgttaaaa aaaggtataa atgtgagttt aggtcttgac ggtgcagcaa
     5581 gtaataacgc acaagatatg gtagagctta tgaaattaac agctttacaa cacaaagtaa
     5641 ataattgtga ccctctggca atttctgcag agaaagttct tgaaatggct acaattgagg
     5701 gagcaagaac tcttagaatg gaagatgaaa taggttcttt ggagattggg aaaaaagctg
     5761 acctagtaat atttaactca atgctttctc caaaatcaat acctcttcac aatcctgtat
     5821 caactttggt atactcagct agtatgcaca atgtagagag tgttttagtt gatggaaatg
     5881 taatacttga aaatggaaaa gtaactacaa ttgaaagtga agaacaagtg ttgcttaatg
     5941 cacaacatgc agcagaagaa ttatgtaaga gagctaatgt aactaataga atggaaggac
     6001 ataagtggaa tagcttatac taatattaaa ataagattgt aataatattc tattacaaca
     6061 ttagtttatc tgtaggagga gaaaatggaa aaaataaaat taaatattaa tgattacatg
     6121 tatgaaatag aagttgaaaa gaatgaaact cttttgcatg tacttcgtga aaaattaaat
     6181 ctaactggag cgaaacaagg ttgtaatagt ggcagttgtg gagcatgtaa agttttgata
     6241 gatggagttg acatgaaatc ttgtaaactt cttgcaaaaa actgcgaagg taaagttata
     6301 tacactatag aaagttttgc taatggagaa aatcttcatc caatacaaga agcttttata
     6361 gaagtcggag ctgtacaatg tggatattgt actcctggta tgattataac aacacaagct
     6421 cttttaaata gaaatgcatc tcctaatgaa gatgaaatta gagaagcttt taaagaaaat
     6481 ctctgtagat gcacaggata tgtaaagatt atagaagctg taaaattatc tgcagctaga
     6541 ttagggggta gataattatg gaaaaggtaa taggaaaatc ataccctcta aaagatgcag
     6601 ctttaaaagt tactggtcaa atgaaatacg tggctgatat gaaaccacaa aatgtacttt
     6661 acgctaagat gcttttaagc cctgtagcac atgcaaaaat aaaaagtata gatacaagtg
     6721 aagctgagaa agtagtaggt gttagagcta tagctactta tctcaataca tcacaaagaa
     6781 aatacaatgg ttcattaaga ttttatgagc acaaaatacc tgaaaatgaa gttatatttt
     6841 cagatacagt tagatttgtt ggtgaccgtg ttgcagcagt agctgcagag actccagaaa
     6901 tagcagagaa agctgtaaaa cttataaaag ttgaatatga agaattgcca gctgtattta
     6961 ctatacaaga tgcaataaaa gaagatgcta tctgcataca tggagattca aatatagttt
     7021 ctcaaaacaa cataaatggg ggagatgtag aaaaaggctt caaggaatgt gattatatat
     7081 ttgaagatga atactctact ccagcaatac atcatagtgc aatagagact catagtgtaa
     7141 ttgcacacta tgattataat gataaattaa ctgtacatac accttgccaa aatacatttg
     7201 gatttagaat aatactatct caaatttttg ggatgtcatt aaataaagta agagttataa
     7261 gacctgctat tggaggctca tttggtggaa aattagaact tacaattgaa ccagtagcag
     7321 ccgcattatc aaaaatgaca agaagaccag ttaaaatggt tcttacaaga aaggaatcta
     7381 tgatatcaac aagaactcgt catgggtctt ttgtaaaatt aaaaactggt gtaaataaag
     7441 atggtgaagt aatagcacaa gacataaaaa tatatacaaa tacaggagca tatgcatcaa
     7501 gtgctttaaa tgttatagga gcacttagtc ataaagtatt taaagtttat aaaataccaa
     7561 atataaagtt tactgggatg cctgtatata caaatacacc aatagcagga gctatgagag
     7621 gctatgggtc acctcaaata tttatggctc aacaagctca atttgcgaaa atagcaaagg
     7681 aaataggtat ggatttagtc gattttcaaa ataaaaatgc agtagagcca gatgatgtcg
     7741 atataatctt ccacggttct cttggaaatc ctagagtatt ggattgtata gagcaaggta
     7801 agaagatgtt taagtgggat gaaaagaaaa aacttccaaa agaagaagga aaatatttaa
     7861 gaggtatagg tatggcaata ggtgcacatg gtaatggagt ttttggtgca catagagatg
     7921 ttgttacatt gactcttaaa ttaaatgaag atggtacgtt gacactacta acaggagctc
     7981 atgatatggg taatggtgtt gtaactatgc aaacaatgat ggtagctgaa gttttaggaa
     8041 taacacctga taaagttgat acatttgaaa ctgatacgga tgcatgtaca ttcaaccttg
     8101 gtgactatgc aagtagaggg gtatttgtag aaggtggagg agctaaaaag acagcagaga
     8161 aattgaaaaa tcttatatta gaagaagcag aaaaattact tgaaacatca aaagaagatt
     8221 tatacctaga caatggatgt gtaattagta aacttgatga aaatgtaaaa gcatcacttt
     8281 ctgatgtcgc agtatatagt caaagtaaat cattaagaga gttaactgtt gttgaagatt
     8341 attcttcacc agctggacgt acttcttatg gtgtacattt tgctgaagtt ttagtagata
     8401 aagaaactaa ggatataaaa gtagttgatt ttgtagctgt acatgatgtt ggtagagtta
     8461 taaatcctct aaatttagaa ggacaattag aaggtgggat tcatatgggt ctaggatacg
     8521 cattgagtga agaattggca tttgatgaac aaggtagacc taaagctact gattttaaaa
     8581 catataaatt atttagaact gtagatatgc caaaatgtca agttgcattt gtagatggtt
     8641 atgaaaaagc tgggcctttt ggtggtaaga gtattggaga atgtgccgtt gtaccagttg
     8701 cgccagctgt tgcaaatgca gtatataatg caactgatgt agagatacat tcattaccaa
     8761 taaaattaaa atagtagaaa ttaaagcaga aataacaatt tattgtattt gtaaatagat
     8821 aataaaaata gatttatttg gtgaggagat attattatgg caaagatatt aggtatttgt
     8881 ggaagtccac gtaaaggagc aacagaatat gcagtgttag aagctttgaa agaagcagaa
     8941 actatacctg gtatagaaac tgagtattgg tcagttagag ggaaaaaaat aagtccttgt
     9001 gtacattgtg atgcatgtat aagaaaagaa actatgtgta ttataaaaga tgatatccaa
     9061 gaattagagc aaaaaatatt ggaggcagat ggaattatag taggttctcc agtttatgat
     9121 atgaatataa ctgctcaact tacagcagta tttaatagac ttcgtccaat gtatttagtt
     9181 catccaggaa aacttcaaaa taaggttggt gcagctataa ctactggtgg aactagatat
     9241 ggtggccaag aattgacaaa acttcctata ataaatttct tcctaatgca tgaaatgtta
     9301 gtgtctggag gtttaggtgg atgctatata ggtggtacaa tatggagtaa agatggaaaa
     9361 gcaaaaggtg cccaagagga tgagacaggt atggacacag taaaaagact tggaaaaggt
     9421 gcagcagagg cagttatggt ttctaagttt ggtcttgaaa aatggaatgt cgtaaaagaa
     9481 gaattagatg ttaaaaaaga tgaaaaatca cctcttagag accactaaag atttattata
     9541 ttattgaata tatacaaata gtgatgtaat agattattgc atcactattt tatgttttaa
     9601 tttagatttt ataaatttta tattaaaaaa tatagaaaca aagtgttttg agatttattt
     9661 ttaaatttta aaaatgagaa ttaaaaagtg agaagataga aaaggttttt tagttattaa
     9721 attctacata cattgtatga actaaagata gaaaagaaaa aataataaaa gcattgcaat
     9781 ttctatacta aagagtcatg gaaaaatatc tagtatatat ttagtattgg catgttaatt
     9841 gcttatatat taaagtataa aaatttaaca ataatttgga ggtattttag atgttaaaag
     9901 aaattaaatg gaaagtaaat aatttgccaa agggcgataa ggaaaattgt atcaaatttt
     9961 taaatgaaga agaaataact aaggttagaa attttcacaa aagctttcct caatataaag
    10021 aaactccatt agcaaaccta gaaggtttag caaaaaaatt aggtgttgca ggagtatatg
    10081 taaaagatga atcttataga tttggattaa atgcatttaa agtattaggt ggttcttatt
    10141 ctatgggtag atacttagct caaagattag atacagatat atctgagtta gggtatgaca
    10201 aattgacttc gaaagaaata aaagaaaaat taggagaaat aactttcttt acagctacag
    10261 atggaaatca cggtagagga gttgcttgga cagctaataa attaggtcaa aaatctgttg
    10321 tattaatgcc aaaaggttct tctgaattta gattaaataa aattaaagga gaaggtgcag
    10381 atgctagtat aacagactta aattatgatg atgcagtaag attagctaat gattatgcag
    10441 aagctgatga ccatggagta atggttcaag atacagcatg ggacggatat gaagaaatac
    10501 cagcatggat aatgcaagga tatggaacta tggctcaaga agctatagag caattaaaag
    10561 aatatggagt agatagacca acacacgtat ttgttcaagc aggagttggt tcacttgctg
    10621 gtgcagttca agggtatgtt gcttcaatat atgatgaatg cccaataaca gtagtagtag
    10681 aggctgatga agctgattgt tactataaat cagcagaagc tggagatgga aaaccaagat
    10741 ttgttggagg agatatgcca actataatgg cagggcttgc ttgtggagaa ccaaatacta
    10801 taggatttga agtattaaaa aatcatgcag cagcatttgt ttcagcacca gactgggttt
    10861 ctgctaaagg tatgagaaca ttaggtaatc ctttaaatgg tgatgaaaag gtaatttctg
    10921 gagaatctgg agcagttaca actggtcttt tagtagcagc tatggaaaga gaagatttag
    10981 cagatttaag aaaagattta aaattagatg aaaattcaag aatactttta ataagcacag
    11041 aaggagatac tgaccctgac aaatatagaa gtatagtttg ggatggagaa tatccaagta
    11101 tataaggaat tactaaaatg caattattag atattggttt acatatttag aagatatata
    11161 agttctaaaa tttagaaaat atataagtat ataaaataca tacattataa aaaatctagg
    11221 gggataatga aatgttaaac gaacaaagaa aacaagaagt aactgaaata tgccaaaaat
    11281 taattcaaaa tcctagttct tctggaaatg aagaaggtgt tgtaaaagca atagaagagt
    11341 cttttgaaaa attaggtttt gatagttggt caagagatag atatggaaat atagtaggat
    11401 gtataaaagg taacaagcca ggtaagaaaa tattatttga tggacatata gatacagttc
    11461 cagttccaga tgcaagtaaa tggagtgttc ctccttttga aggaaaaata gttgatggaa
    11521 agatatatgg tagaggaact tctgacatga aaggtcaata tactgcaatg atgtctgctg
    11581 tagcgtattt tgctaaagac actaatagag attttgctgg agaactatat gtagcaggtg
    11641 ttgttcatga agaaatattt gaaggtgttt cggctagaga gataagtaag gctgttcaac
    11701 ctgactatgt tgtaataggt gagtcttcag agttaaactt aaagattggt cagagaggta
    11761 gaggagaaat agtagtagaa acttttggta aaccagctca ctctgctaat ccagaaaaag
    11821 gt
//
LOCUS       DAGSRM010000114        11652 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.115, whole genome shotgun sequence.
ACCESSION   DAGSRM010000114 DAGSRM010000000
VERSION     DAGSRM010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11652
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.115"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            130..906
                     /locus_tag="MJE33_003222"
     CDS             130..906
                     /locus_tag="MJE33_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBY9649138.1"
                     /translation="MEYIIFDLEFNQGFDKKLNKTVSNEKCPFEIIQIGAIKLDSKFN
                     IIDTFNSYVKPTIYKDIHPFISRMTNIKSSDFNDSPTFPEVYNNFIKFISSQDPILCV
                     WGAGDLKELYRNINYHKLPSNSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILELD
                     EKMSYHNALNDAYYTAKVFIKIYNPSIVPDIYLYTSIKPKTIRYSNKKRVDYDKLFDE
                     FRKILNRELTKDEKKIINLAYNMWKTNQFTLENVKQRKNK"
     gene            complement(998..1750)
                     /gene="xth"
                     /locus_tag="MJE33_003223"
     CDS             complement(998..1750)
                     /gene="xth"
                     /locus_tag="MJE33_003223"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBY9649139.1"
                     /translation="MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLVSADIHTEILGSDHCPVELEIK"
     gene            complement(1911..2861)
                     /gene="galT"
                     /locus_tag="MJE33_003224"
     CDS             complement(1911..2861)
                     /gene="galT"
                     /locus_tag="MJE33_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HBY9649140.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDEEC
                     PFCKGNEAHATDALFEIDSEEGWLVKSVYNKFPIIDDMARDVYGVHEVMIESDKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLDRRVVYNGEAFLVLIPYATKYSGE
                     VRIIFKDKIEFGELNDNNIQELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEDLTKKLKID"
     gene            3246..4442
                     /locus_tag="MJE33_003225"
     CDS             3246..4442
                     /locus_tag="MJE33_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBY9649141.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYDMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALQK"
     gene            complement(4777..5229)
                     /locus_tag="MJE33_003226"
     CDS             complement(4777..5229)
                     /locus_tag="MJE33_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBY9649142.1"
                     /translation="MNYIVKDTISFLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRA
                     LVFIKNYGVISMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITEE
                     GDKIATDFMDKLSDGLGEKLSKLTEEDRKKYLEAINTLQYILNKEEFR"
     gene            complement(5540..6985)
                     /locus_tag="MJE33_003227"
     CDS             complement(5540..6985)
                     /locus_tag="MJE33_003227"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBY9649143.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYHSSYAVSGQTYTRKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINRRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
     gene            complement(7129..8037)
                     /locus_tag="MJE33_003228"
     CDS             complement(7129..8037)
                     /locus_tag="MJE33_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBY9649144.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLDINKK"
     gene            8294..9118
                     /locus_tag="MJE33_003229"
     CDS             8294..9118
                     /locus_tag="MJE33_003229"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBY9649145.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILVDEFIKLNF
                     SNTFISTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRLFFNSTVVAIALIVYGIIMIGLEKRNKKPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            9158..9688
                     /gene="lepB"
                     /locus_tag="MJE33_003230"
     CDS             9158..9688
                     /gene="lepB"
                     /locus_tag="MJE33_003230"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBY9649146.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSIVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRVIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKLFAMGDNRENSNDSRFPDVGMVDEDEVLG
                     KVMVRLLPLDNIGKVD"
     gene            complement(9770..11551)
                     /locus_tag="MJE33_003231"
     CDS             complement(9770..11551)
                     /locus_tag="MJE33_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HBY9649147.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINEDVKVVPKVTPFKGIN
                     TDLLYVKDGRILFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLEEYLNDENDEINLNLFLLDIC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEDVQIKDVVSIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLVIFTHTKQLCNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKTFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSCNSLNISNRLNIFKKLYNGIEFDDKIILSKNYRQAKEIVDFT
                     NKFSNNSNSYINELRPNTEFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYL
                     ISKKGLDYSEVAIVYPYNKKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKK
                     VGTTIANIYAIKNLEYKAVIVCELEMLYNQTINDIEQDYQVNDFVGDLNKVYLAMSRA
                     TDYLSIVTTFNEEASDIIRLITESKDI"
BASE COUNT         3927 a         1747 c         1414 g         4564 t
ORIGIN      
        1 acaatagttt aacattttta caaatatggg gtatactaaa ataaaagtat ttttatttga
       61 aaatttaatt ttatttataa tataatctat agtaaaagaa ataataaaaa aatttaggag
      121 ataaacttta tggaatacat aattttcgat ttggaattta accaaggatt tgataaaaaa
      181 ctcaataaaa ctgtatccaa tgaaaagtgt ccttttgaaa ttatacaaat aggagcaatt
      241 aaattggatt ctaaattcaa tataatagat acgtttaata gctatgtaaa acctacaatt
      301 tataaagaca tacatccctt tattagtcgt atgactaata ttaaaagttc tgattttaat
      361 gattcgccta ctttccctga ggtctacaac aattttataa aatttatatc atcacaagac
      421 cctatattat gtgtttgggg agctggagat ttgaaggaat tatacaggaa tatcaactat
      481 cacaagctac catcaaattc cttacctaaa tcttatatta atatacaaca acatgcatct
      541 aaatatttca ataatccagc aggaaaaagc attggcctac aaaatgctat ttctatattg
      601 gaacttgatg aaaaaatgtc ttatcacaat gctttaaatg atgcatatta tactgctaaa
      661 gtttttatta agatatacaa tccaagtata gtcccagata tatatcttta tacgagtata
      721 aaacctaaaa ctataagata ttctaacaaa aaaagagttg attatgataa gttatttgat
      781 gaatttagga aaattttaaa tcgtgaatta actaaagacg agaaaaaaat cattaactta
      841 gcttataata tgtggaaaac aaatcaattt acattagaaa atgttaagca aagaaaaaac
      901 aaatagagtt tatttagata aaaaataggc atgtagatta aaaaactagt taactttaaa
      961 tttatatcta cagcccaatt tttatcataa ttaattttta ttttatctct aattcaactg
     1021 gacaatggtc tgaacctaat atttcagtgt gtatatccgc acttactaat ctatcttcta
     1081 attttttgga tgcacaaaaa taatctattc tccaccctgc attatttttt cttgcattaa
     1141 atctatacga ccaccaacta taaacacctt ctttatcagg attaaaatat ctatatgtgt
     1201 ctataaaccc actgtctaat aatttagaaa acttctctct ttcttcttta gtaaaacctg
     1261 catttttcat attattttta ggatttttta aatctatttc tttatgtgca acgttcatat
     1321 ctccacacac aattacaggc ttatgattat ctagtttggt taaataatct ataaagtcgt
     1381 cttcccatct agttctatat tctagtctct ttaattcaga ttgagaattt ggcgtatata
     1441 ctgtaacaaa gtaaaaatct tcaaactcta gagttatcac tctaccctct ttgtcatgtt
     1501 cttctatatt tattccatac attactttta aaggctcttt ttttgtaaat atagcagtac
     1561 ctgaataacc ctttctctca gcataattcc aatattgaaa atatcctggt aaatctaact
     1621 ctatttgccc ttcttgcaat ttagtttctt gtaaacaaaa tatatctgca tctacttctt
     1681 tgaaaaagtc taaaaatcct ttcccaacac aagctcttat tccattaaca ttccatgata
     1741 taaatttcat acttatactc ctcatctaaa cattgacaca ctccccatag ctaaagatgg
     1801 gggcttttca acaattttat tgtaaattct taatttactt ttattttaac aatgctatac
     1861 tataatttca tctaaaatta ctatttactt aatacctaaa aatatatgtt ttaatcaatt
     1921 tttaactttt ttgttaaatc ttcagggctc attgaagaaa catacatatt tgttccaagt
     1981 tcaaatcctc caaaattata ttttcttggg attatgtgca tatgaacatt aaaatacgat
     2041 tttgtttcaa tctttgttgg atgagtgtga atacataaat taaatggcat atatccattg
     2101 atattgtata acttcttaaa caacttttca aatattgtag aaagctcctg tatattatta
     2161 tcatttaact caccaaactc tattttatct ttaaatatta ttcttacttc tccactatac
     2221 ttggttgcat atggtataag taccaaaaaa gcttctccat tataaacaac tcttctatct
     2281 aaacttattt cattttctat taaatcatca tacaaagagc ttttattgct gtcataatac
     2341 tcctttgaaa ccaacagttc attcgtaatt tcaggtggaa taaaagacat agacaatatt
     2401 tgagcatgtg gatgcatcaa agatgctcct gcctttctca agaagttttt gaagatacta
     2461 acatactcta ctttatcatc tttcgacaaa tctctaaatc tatttctata cataaaaaaa
     2521 acatcttcaa attctttttg actcatgtta taaaaactac gattatgttt atcgctctct
     2581 atcattactt catgaacacc atatacatct cttgccatat catcaattat aggaaattta
     2641 ttgtagacag attttacaag ccatccttct tcactatcta tttcaaataa tgcatcagtt
     2701 gcatgagctt catttccttt gcaaaatggg cattcttcat catattcatt agtttcttca
     2761 tcttcatttg ggatatctgc tgtatctaaa ggtcttttta acctatctgt agcaaatata
     2821 acaacatcat tggtaattgg atctattctt aattctttca taattaacac ctcttagtta
     2881 atagccttta atataattat atgatattta tcgtatctgt taaataaatt tataatattt
     2941 tttataattt tgcatattta ttattatata ttttgtctat agctgaaaga ttataactaa
     3001 aaaatcacta tcagtacatc agaatattat cttactataa attaatttat gaatacataa
     3061 tttagtggca taaacatggg tattttacta taatttgtta aaagtttatt ttaaagctat
     3121 attaattttt tttgatgatt tttacccata agtatgttat tattatcttg taaagatgtc
     3181 ttgtattcaa acatacaatt tacataatac aatatacgta tttaaattaa atagggggta
     3241 ttaaaatgaa ttattcaaac agagttagtg caatgcaagc ctctccaatc agaaaacttg
     3301 ttccatttgc acaagcagca aaagataaag gaatcaaagt atatcactta aacataggac
     3361 aaccagacat aaaaactcct aaaggatttt ttgatgctgt taaaaacttt gatagcgaag
     3421 tcttagaata tgctacatca gaaggtattc cagaattatt agaagcactt caaaattact
     3481 ataaaactta cgatatgaat tttgaaaaag atgaattatt agtaactaac ggtggtagtg
     3541 aagctttact tttcactatg atggcagttt gtgacccagg agataatcta ttagttccag
     3601 aaccattcta tacaaactac aatggatttg gtcaatctgt aaacgtagaa gttaatgctg
     3661 ttacaacaaa ggcagaaaac ggtttccacc ttccatctaa agaagaaata ttgtctaaag
     3721 tagatgataa aactaaggct ataattcttt ctaacccagg aaatccaact ggtgcaattt
     3781 atacaaaaga agaacttaat atcctagctg aaatagctaa agaaaaagat ttatggataa
     3841 tagcagacga agtttataga gaatttgttt atgatgggct tgagtataca agctgtggta
     3901 acttagaggg tgtacaggat agagttataa taatagacag tgtttctaag agatacagtg
     3961 cttgtggtgc tagaattggt tctatcgctt gcaaaaataa aggtcttatc gctcaaatac
     4021 ttaaattatg tcaaggaaga ctttgtgttc ctacattaga gcaaatagga gctgttgagt
     4081 tatacaaaac tcctgtttct tacttcaaag aagttaatga agagtacaaa aaaagaagag
     4141 atgtattata caatgaatta atgaaggttg aaggtgttat atgtaaaaaa ccaactggtg
     4201 ctttctatat agttgctaaa ttaccagttg aaaatgcaga agatttcaca atttggatgt
     4261 taaaagaatt taataaagat aatgaaacag ttatggtttg ccctgctgaa ggattctatg
     4321 ctactccagg tttaggacgt gatgaaataa gattagctta catcttaaat gaaaaagacc
     4381 ttcatagagc tgctacatta ttaaaagaag gtttagagca atatgtagct ttacaaaaat
     4441 aattttttaa acttaattta tataaattta atgatataaa atgtcccctt ttcagatatt
     4501 atttgaaaag gggacatttc ttaattaata tattaattaa gacttagacc tgactatttt
     4561 tcattttctt tatatagtgt tggccagtaa tctttattca ctttaattaa agcatctaaa
     4621 acttgtcttg ccttagttag agaaattata aaaaagtaca tcaagaaaat ttaagatgta
     4681 ctttttagat tgtatttaga cttataatta tatagttaaa taaataacaa agaattaatc
     4741 ttagtaccat taaatacaaa aatttttata ttttttctat ctaaattctt ctttgtttaa
     4801 tatatattga agagtattta ttgcttctaa atattttttt ctgtcttctt cagttaattt
     4861 agaaagtttt tctcctaaac catcacttaa tttatccata aaatcagttg ctatcttatc
     4921 tccctcttca gtaatgtcaa ttagatattt tcttctatca actaaatctc ttttccttgt
     4981 tacatacttc ttatcagtta aatcatcaac catactagtt aaactgcctt tttctatatt
     5041 taacttagag cataaatcag tcatacttat tactccataa ttttttatga aaactaatgc
     5101 tcttaattgt gttttattta cattataatc tggtgcaaat ttttttaggt cttctaagta
     5161 caggctagaa taaatcttag gaaatgtttt tactaaaaaa cttatggtat cttttactat
     5221 atagttcata taatcacctc ggttgtttta aaatttatta aattatctaa ataactttat
     5281 ttcagtttac attaaaaata ttgcaagtac aatagttttt gctgaatgtt taaatttaat
     5341 tagtaatctt atcatttttt tatttttatt tatattattc ctattaaata ttatttatat
     5401 agctatttta ttaaattcat ggtatagtaa gtaaagaaga actcttcaca gcggtggaga
     5461 gaagacgcta gcccattaaa atggctatct tatttataaa taagatagcc gtttttaatt
     5521 atattttatc tgtacaattc tatactctta ggtctacatc tacttttcct aagtcttcct
     5581 tataatcttt tatagcaggt tctacaactt catttacaaa ttctacaact tgttctggag
     5641 ctctacctat aaagtttttt gggtccatta tagatagtat ttcttccttg ctcattttaa
     5701 atgaatcatc attcattata agttcaatta agttattatc cttgccttca tgctttactc
     5761 tataggcagc tttcatagaa tattctctta ttatttcatg caattcctgt ctatctccac
     5821 ctctttttac agcttccata agtattgttt ctgtagccat aaaaggaagt tcttcattta
     5881 cacgtctatt tatcatattt tcatatacta ctaaaccatc agttacattt atccctattt
     5941 caagtatagc atctgctgcc ataaatgctt gaggtattgc caatctctta ttggctgagt
     6001 catctaatgt tctctctaac cattgagtgg cttgtaccat tgcagggctt atagattcag
     6061 agattatata cttagataaa gatgctattc tctcacttct cattggattc ctcttataag
     6121 ccattgctga tgaacctatt tgattttttt caaatggctc ttctaattct tttaaacttt
     6181 gtaaaagtct tatatcatta gtcatcttat gcatactttg agctatccct gatagtatgc
     6241 ttattacttg ataatctaac tttctagtat atgtctgtcc acttactgca taggaagaat
     6301 ggtatcccat ctttttacaa attttcttat ctaactcttt tactttttcg tggtctcctt
     6361 caaataagct taaaaaactt gcttgagtac cagtagtacc tttaactcct cttaacttca
     6421 tgttgtctat tctatagttt aaatcttcca aatcaagaat taaatcttga gcccataaac
     6481 aagctctttt tcctactgtt gtaagttgag cagcttggaa gtgtgtaaat ccaagagctg
     6541 gcatatcttt gtacttaagt gcaaattctt ttaaattgtt tattaaattt acaagcttta
     6601 ctcttagaag ttttaaagct tcattcattt gtataacatc tgtattatct ccaacatatg
     6661 cagatgttgc tcctaaatgt attattcctt tggctttttc acattgtaaa ccataagctt
     6721 ttacgtgaga cattacatca tgtcttactt ctttttctat ttttctagct tcatcaaaat
     6781 ttatattatc tatattagct cttagctcat ctaattgttc ttcagttata ttgattccta
     6841 actcatgttc accttctgca agtgcaaccc ataaccttct ccatgtagag aatttaaatt
     6901 gtggagaaaa tatataactc atgtccttgc tacaatatct gtctgtaagt gggtttgagt
     6961 aagtagtata tttattatct atcattatat tagcaccttc cttaaatctt ttgtcgtgtg
     7021 attactgtgt acacattagt acataaataa ttatatataa ttttacgaac tataacaaga
     7081 cttttttatt ttttgttcac atttatatat agtatttttt atttattact attttttatt
     7141 tatatccaaa ttatcttttt caattaagta atccactatt tcactatcaa caaaatttaa
     7201 tacatactta tatctaattg catagtattt atcactccat ccaagctgat tttttacatc
     7261 actttcatct gccccaagcc ttaaacttat tgctgcaaat gaatgtctaa aatctcttgc
     7321 tgaatattga gaaagtccag ctaaatctaa tgcttttttg actataagtc tgacatttct
     7381 atctgttata gaattactct tttgagttgt aaaaataaaa tcatcactag gtattattac
     7441 ctctggaagc ccagaataat tcctgtaatc ttctaaaagt ttaaaaaagt atggatgtaa
     7501 tttaactact ctttcttttc ttcccttacc taaccttaca tatgagttat cgttttcatc
     7561 taccataagg tctttccact ttaagctaac taattcattt aataaacacc cagtagtaac
     7621 tagacagact attattactc tatctcttat atttaatttt ggtaggatac ctataagttt
     7681 atttatttct tgtatactta gaacatcatc ttttgttttt attcttgtta ctgtgggttt
     7741 ttctacatat ctaaatggat ttatatctat gtatccttct ttttttaaga agttataaaa
     7801 actatgtaaa taactatata tcttttcaca agttgatttc gcatatttag tctgtatata
     7861 gtctacaaaa tttttacaat cttctttcgt agcataaatc aattctttcc cttttaaaaa
     7921 ttctttaaac agtataacct ttgataaata atcgtgtttc gtgtttggat ttaattttgt
     7981 agaaaaaaca ttaaaagttt catactcata atcatctagt atttttgtac tatacataat
     8041 aactcctttc cttttcataa aataaaatag ctatactttt actataccat atatttcccc
     8101 ctcaaatata ccccttattc aatagataac ttttaagttt attttgttat attatgaagg
     8161 ttttatgaaa atatcaatct tccattgtgc actacacagt actatggtat tataagttag
     8221 taaaaggtgg cattttttta ttaatctatg aataaaatat gctactataa atttgtattg
     8281 gaggtatttt gatttggaaa catttagttt gcttgaaatt tttaaggcag tcattttggg
     8341 catagttcag ggaataacag aatggctacc agtaagtagt acagggcata tgatattagt
     8401 tgatgagttt ataaaattga atttttcaaa tacatttata agtacatttt tagttgtaat
     8461 tcaatttggt tcaatattag ctgtacttgt tatatttttt agaaaattaa atccttttga
     8521 tagcgcaaag aatattaagc agaaaaaaga aacagtccgt ttatggctta aggttataat
     8581 tgcagttata ccatctggag ttattggaat actgtttgaa gatgatatag atagattatt
     8641 ctttaattca acagtagttg ctattgctct tattgtatat ggtataataa tgatagggct
     8701 tgaaaagaga aataaaaaac ctaagtataa agatttttct caagttacat ataaattagc
     8761 tctttgtata ggattattcc agtgtctagc tcttatacca ggtacatcta ggtctggttc
     8821 tactataata ggtgcagtat tacttggtac ttctagatat gtagcagcag agttttcatt
     8881 cttcttagcc atccctacaa tgcttggagc tagtgcatta aaacttctaa aagctggatt
     8941 tggatttact ggatttgaat ggcttatttt aggtgttggt tctgtagtag catttgttgt
     9001 ttcaattgta gttattaaat tttttatgga ctatatcaaa aaacatgact tcaaagtatt
     9061 tggttattat agaatagtct tgggtatagt ggttcttgct tatttctttc tattataatg
     9121 tattctaatt taataacaaa tgataaggag aaaagttatg agtgttaaaa aagaaatatt
     9181 tgattggatt aagtcaatag ctatggctat tgtacttgca tttgtaattc tacaatttat
     9241 aagaccttct attgtaagtg gagaatcaat gtatcctact ttagatgata aagattatct
     9301 gattttaaat aggatatcat acaaggttgg taaacctgaa aaaggcgata ttgtagtttt
     9361 taaaaccaat ttagttgatg gagaaacagg aaagaaaaaa gacttaataa aaagagttat
     9421 agctactgaa ggtgacagaa taaaaatatc aaattctaaa gtgtatgtaa atggaaaatt
     9481 attaaatgaa ccatatatac acaataacta tacttctgga gatatagata ctgttgttcc
     9541 aaaaggtaaa ctatttgcaa tgggagataa tagagaaaat agtaatgata gtagattccc
     9601 tgatgtaggt atggttgatg aagatgaagt tcttggtaag gttatggtga gactattacc
     9661 tcttgataat attgggaaag tagactaaaa aataagagac tgaattggta aagtttattt
     9721 tacaaatctc agtctctttt tattttctta aattacaaat atttttttac taaatatctt
     9781 ttgattctgt aataagtctt attatatcac ttgcttcttc attaaaagtt gttactatac
     9841 ttaggtagtc tgttgctcta ctcatagcta aataaacctt atttaaatct cctacaaagt
     9901 catttacttg ataatcttgt tcaatatcat ttatagtttg attgtataac atctctaatt
     9961 cacatactat taccgcttta tactctaaat tctttattgc atatatattt gctattgtag
    10021 ttcctacctt cttacttata tttgtcaaat tatcttctgc acatatataa ggaatttttg
    10081 cctcttccaa tgcttttttt aacatatatt gaaagtaaat ggtttttcca ctttttaatt
    10141 tcttcttatt gtaaggataa acgatagcaa cttcagaata atcaagacct tttttactta
    10201 ttaaatactc aatttcccag attacagaac ttatttggtc atcaaggtca cttactttta
    10261 ttatatcaac agacttattt ccacctctaa gtgctttagt ataaaagaaa gtactaaatt
    10321 ctgtatttgg tcttaattcg ttgatatatg agttagaatt atttgaaaat ttatttgtaa
    10381 aatctacaat ctctttagct tgtctataat ttttacttaa gataatctta tcatcaaatt
    10441 ctataccatt atataatttt ttaaatatat tcaatctatt agagatattc aaggagttac
    10501 aagaacacac attaaatatg aattttgttt tatataaaaa ctcccttata aaatctattt
    10561 catactctaa aaagctttct gcttcatcaa taaaaatagc cttgaacata tttttatttt
    10621 ttataatgtt ctgtgcttgt tttacaagat tattaaaggt cttttcgtaa tcatttttta
    10681 acatgttata atctacaatt aaattgaatt ttttagctag tttaaatata aaagaactaa
    10741 atgtgtgaac ttctaaattg ttattatctt tgtataataa ctctatcctc tctcgaagct
    10801 cattgcacag ttgttttgta tgtgtaaata tgactaattt atgatgtgga tatacttttg
    10861 ctaattttat agccctagaa agcataattg ctgttttgcc tataccagaa ccaccttcaa
    10921 taagtgtatt cccatattta atagaaacaa catctttaat ttgaacgtct tccatcatag
    10981 ttgctgtata cttatagtca tcattgtaga atgaaatttt tttaaatttt tcatttagat
    11041 gaagtttatc atttattata tagtattctg aacatatgtc tagtaaaaat aaatttaagt
    11101 ttatttcatc attctcatca ttaagatatt cctccagcga acccttattc attatatctt
    11161 gaagcttatt tttatctatt atattattat taacaaattc ttcaaattca tatgtttcct
    11221 caacttcaac gtatggcata atgtaaacgt agttataact tatatttcta ttcttttgtc
    11281 ccatttttag tttcaaaagt tcatattcct cattcataac ttcaagtaac tcttcttcta
    11341 aaataaaaaa tatatcttct gttgtatcca taaattttat aaataaaatt cttccatctt
    11401 taacatacaa caaatcggta tttattcctt taaaaggagt aacttttggc actactttaa
    11461 cgtcctcatt gatattttcg taaaatattt tctcgaactc cttcaatttt agggcgtcta
    11521 actgagaatt tatatttatg tattggctca cttacattac ctccttctgt tatacttaat
    11581 tataccattt tgaactatat ttttatatat actttttata ggcaaattta gtaaataaat
    11641 tcaaaataaa ta
//
LOCUS       DAGSRM010000115        11462 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.116, whole genome shotgun sequence.
ACCESSION   DAGSRM010000115 DAGSRM010000000
VERSION     DAGSRM010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11462
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.116"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            102..413
                     /locus_tag="MJE33_003232"
     CDS             102..413
                     /locus_tag="MJE33_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBY9649148.1"
                     /translation="MYFTYIIRCKDDSLYTGYTSNIVRRMNEHKLGINSKYTRAKGFE
                     KLEVYFVTNTKSNAMKLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGKEIEQL
                     T"
     regulatory      506..598
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            698..1492
                     /gene="thiD"
                     /locus_tag="MJE33_003233"
     CDS             698..1492
                     /gene="thiD"
                     /locus_tag="MJE33_003233"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBY9649149.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAIT
                     AQNTKGVFAVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGEEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     gene            1516..2304
                     /gene="thiM"
                     /locus_tag="MJE33_003234"
     CDS             1516..2304
                     /gene="thiM"
                     /locus_tag="MJE33_003234"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HBY9649150.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVKGKGVDSFDEEEDSTEIIRKIAEKLECVVV
                     ATGKIDIITNGKGTYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLT
                     MSLSGELANLNNPPIGTFKENLMNAIYQMDIDTLSKNSNIEFLN"
     gene            2327..2959
                     /gene="thiE"
                     /locus_tag="MJE33_003235"
     CDS             2327..2959
                     /gene="thiE"
                     /locus_tag="MJE33_003235"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBY9649151.1"
                     /translation="MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITKRYGVKFIINDRVDIALICDADGVHVGQSDIDVREVR
                     KLIGNNKILGVSARTLEEAICAKNDGADYLGVGSIFTTSTKLDAKSASFETVKEIKEK
                     VDMPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI"
     gene            3098..3400
                     /locus_tag="MJE33_003236"
     CDS             3098..3400
                     /locus_tag="MJE33_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBY9649152.1"
                     /translation="MIRILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGDIKS
                     ELDNFDILLLGPQLRFMLDDVKSIVGDRAPVSIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(3466..4221)
                     /locus_tag="MJE33_003237"
     CDS             complement(3466..4221)
                     /locus_tag="MJE33_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9649153.1"
                     /translation="MGMLNILWRSMKWRFKNPISFVVTILQPFLWLVLYSSIANQTMN
                     NININNYTAFILPGIIVLVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQL
                     LEAILVSFIEVTILCIVSIFFSVRIESGIGGILLMIVLIFMTAFFLSSLAYSISLLLP
                     NEIVYETIMTAIVLPIFFLSSALFPIESLSGGLKVAVMLNPFTHVINALRSLIFGETI
                     LIGDIMPVILLFLILCCSSFSLAMWRLKKEMVS"
     gene            complement(4209..5171)
                     /locus_tag="MJE33_003238"
     CDS             complement(4209..5171)
                     /locus_tag="MJE33_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9649154.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNALSFNVNAGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVTMFGKDLVDNPSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDAIKSTGLVKFMSVRENSIFISVNDSRTAF
                     TLINKWLLEHDIEFDAIEIVEPSLEDVFLALTSSKKSLKEEWEC"
     gene            complement(5175..5633)
                     /locus_tag="MJE33_003239"
     CDS             complement(5175..5633)
                     /locus_tag="MJE33_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBY9649155.1"
                     /translation="MNCKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHTGSYSNFPKT
                     YEMIFKYIHENNIKIQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            5988..6344
                     /locus_tag="MJE33_003240"
     CDS             5988..6344
                     /locus_tag="MJE33_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY9649156.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVTGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            6416..7132
                     /locus_tag="MJE33_003241"
     CDS             6416..7132
                     /locus_tag="MJE33_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9649157.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIEALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            7125..7949
                     /locus_tag="MJE33_003242"
     CDS             7125..7949
                     /locus_tag="MJE33_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9649158.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLSLNLFEINKFKNS
                     KFIMNLAIEDFGGIFYGLGILSNLGFIQSILFFGILGLFIYYIFMWKREFFSNNQSIY
                     TLMMLPQNKFKICISKSIALITMIYGFLISQTATLFICKYIFNFIFRNMPIINMNFAK
                     DLYYLNLKSIPIDFISFMAIYVFLLLIAISIVNCCILFIFSYINKFYVILITLLIIAG
                     LYFSTIYINIITLFRKYYSLIGFQFNNLVAGLLSFLIIMFILNGISYLLIKKKVSL"
     gene            7961..9160
                     /locus_tag="MJE33_003243"
     CDS             7961..9160
                     /locus_tag="MJE33_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649159.1"
                     /translation="MKLKSKISILIIFLTLAIYFSIGLLNNEDNLKIKTIKGDSNELD
                     HININFGYALNGYINSYYTINGSNKTKKTELIKLQKDSKNTNNLIDVSSGSDLIAHVG
                     FEMNEESKDINFVIDEKKEDLEKGVFPSEGSDKEIEYEKLDMNNFTRTRYKVDKSTDD
                     YYANILASKRYKKYLYVSVLYATSDSSENNKEYSSTIDIIKINPENKKIKTIKSINLS
                     NYFSEKYQNIETIGVSYASKYEDKMYILVNVLNTNIGDNSNSKFHLLEYNLEENKESI
                     MELKTDSKMALAKAKLEANKLYMVMSNDYDNSSITELNLLTYNILDKSLDEDKYVLDN
                     REKRGIRSIEIDSNKIVAYTSRLNSILSWDNKFMLYIINKNSKEIVYEGELQFNSSNS
                     MLEFEIK"
     gene            9169..9981
                     /locus_tag="MJE33_003244"
     CDS             9169..9981
                     /locus_tag="MJE33_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649160.1"
                     /translation="MKDLIRLFEFELKRNIKNYIFIIICCCSFVILNIVKNLNDYNYI
                     IENAVKSEQLTKIGNTIETVKVTGFSSFRNIMGSTESWFMFGIIACICYAFFIWYRDF
                     NGRSKSIYTLIMLPKNRINIYISKLLNILFLVYSYTVVLTISLFIASKLLPRHMLGNV
                     TNYGFVQETIYELKMLLPYSFEILFVEYIFLLIGFVSVVFTSILINKSIYKVSTLISF
                     LFLVIEILVFIISIEFIIPYEYSDVFTVLYSSINIFVCSLISNKLLKQKIDF"
     gene            9992..11260
                     /locus_tag="MJE33_003245"
     CDS             9992..11260
                     /locus_tag="MJE33_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649161.1"
                     /translation="MKAKNIGKYTICFIIVCFLMITMTLILSYFNKDKLSVKTLKGNV
                     KDIGRVSVVYTPNISKFSYEEVIISKNGVRNNTNPKSRIRNDYLNIDKKYSDFVNDKG
                     KEFIYKDDLNIGEAYTSVKSDLVQGMNALNDCIKVSNKNLKSGVEENFDINLNKSDFK
                     ENSWKTLFNKIYKGNLYMVISETNISGSKVNNICILKIDIKNKSYKLLNRFNLGLQNG
                     EQQNINEIKFSIGSKLYLEIVNNKSKNGYIEGHDSYSNFIIYDIEDNTFNISNKFMDN
                     LNKEKNANPKEIGLLSKTKLDYMVKNNKLNLIVTDMSNSITNLVYDINSNEIKLEDYE
                     ILDLKVDYNEASSGVRKVKLLDDKIYSISGIKNSISGELKGFKGIVDVVGTKPVKFQV
                     FDMKLKKNIYEAELINGDISLQEKLFFVAN"
BASE COUNT         4593 a         1192 c         1807 g         3870 t
ORIGIN      
        1 ttataattaa ttctttgaag attgcatatt caaaatacac accatttttt ttaattctat
       61 aatataatat ttatatattt aatgttagga tgtgatttaa ggtgtatttt acatatatta
      121 taagatgtaa agatgattct ctatacactg gatataccag taatatagtt agaagaatga
      181 atgaacataa attaggaatt aattcaaaat acacaagagc aaagggattt gaaaaactag
      241 aagtttattt tgtgaccaac acaaaaagca atgctatgaa gttagaatac tatataaaaa
      301 aattaactag aaataaaaaa ttgtctataa taaaaaaccc aagtatactt ataaatttaa
      361 ttgataataa agaagattat attattggta aggaaataga acaattaaca taaatattta
      421 tagtaacatt ataatgggtg catatggtgt aaaaacttga caataaatac tattgtaata
      481 taatgaagtt ataaatatag taaattaaaa tagctagggg agccaagagc tgagaggtat
      541 accgaccctt acacctgatc tggataatac cagcgtagga aagcttagta aaaattcaag
      601 tatgtattat ttgtgatata tgctatttct aagctatacc tattgtaggt atagcttttt
      661 taattaagtt atttaaatta aactttagga ggcaacaatg agtaattata aaataccaac
      721 acttacaata gccggctctg attcatctgg aggagctggg attcaggctg atttgaaaac
      781 gtttagtgca attggtactt atggtatgag tgtaataaca gctataacag cacagaatac
      841 taaaggagtt tttgctgtag aggatttaaa taagaaaata ataaaaaaac aaatagaggc
      901 agtctttgaa gatatacctc caagagctgt aaaaatagga atggtttcaa gtcctgagat
      961 tatacttgaa atagttgaga atctaaagaa atacaatcca aaatatttgg ttgtggaccc
     1021 agtaatgata tctaaaagtg gatattatct attaaagcca gaggcaaaag aaaacttaat
     1081 taagtatttg ataccacttg catacataat tacaccaaat ataccagagg cagaagaaat
     1141 aacaggaata aaaatacata atgtagatga tatgaagaga gtaggagaag aaatactaca
     1201 acttggacct aaatttgtac ttatgaaagg aggtcattta gatggagaag cagtggatat
     1261 tttagttggt aaaaatatat ttaaagttta taaaagtgaa agaattgata aaaaaaatac
     1321 acatggaaca gggtgcacat tatcttctgc aataacatct tatctagcat taggatatga
     1381 aataacagaa gcagtaaatc taagtaaaat ctatataaca gaagcaataa aaagaagttt
     1441 tgatataggt catggagtag gaccagtaca tcatttttat aaatttgagt aataaaaaaa
     1501 tattggaggt agaatatgta taatttaatt aaagatgtaa aaaaattaaa tccattggta
     1561 attcattata ctaataatgt aactataaat gattgtgcta atgtaacttt agctgtagga
     1621 gcaagtcctc ttatgagttt ttcatatgag gaagtagaag aaatggtaag tgtagcaaac
     1681 tctgtagtaa taaatatagg aactatgaac tcaaatatgt tggatttatt cctattagct
     1741 ggaaaagcag caaataagta caataaacct gttgttttgg acccagttgg agtatttgca
     1801 agcaaagcga gagcagaact tacaagtaga cttttaaatg aagtcaaatt tagtgttgtt
     1861 aaagggaatg tatcagaaat aaagtttata ggtggcttta atgtaaaggg taaaggtgta
     1921 gattcatttg atgaagaaga agattctact gagataataa gaaagattgc tgaaaagctt
     1981 gaatgtgtag ttgtagcaac tggaaaaata gatataataa ctaatggtaa aggtacatat
     2041 aaaataaaca atggaactga taaattaaaa ggaattacag gaactggatg tatgactgct
     2101 agcttgattg caagttttat ggctgttact gagaatatat tagaagcagc aacaatgggt
     2161 gttcttacaa tgtctttaag tggggaatta gctaatttaa ataatccacc aataggtaca
     2221 tttaaagaaa atcttatgaa tgctatttat caaatggata tagatacttt aagtaaaaat
     2281 tctaatattg aatttttaaa ttaataaaga gtaagggaga cttaaaatga ttgataaaga
     2341 atctttaaaa aaatgtttaa agttgtattt agtaactgac tctgaaatgc ttaaaggtag
     2401 agacttttat aaatgtcttg aagatgcaat atcttctgga ataacaactg tacaacttag
     2461 agaaaaaaat gcttctggaa gagagttttt aagaaaagct atgaaactta gagaaataac
     2521 taaaagatat ggtgttaagt ttataataaa tgatagagtg gatatagcac ttatttgtga
     2581 tgcagatgga gtacatgtag gacagagtga cattgatgtt agagaggtta gaaagcttat
     2641 aggaaacaat aaaattctag gagtatcagc aagaacttta gaagaagcta tttgtgcaaa
     2701 aaatgatggc gcagactatt taggtgtagg gtctatattc acaacatcta ctaaattaga
     2761 tgcaaaatca gcttcgtttg aaactgttaa agaaattaaa gaaaaagtag acatgccatt
     2821 tgtactaatt ggaggtataa atttagacaa tatagataaa cttaaatgtt tagaaagtga
     2881 cgggtatgct ataatatcag ctatattaaa agctgaagat atctctaaag aagtggaaaa
     2941 gtggacatta aaaatataaa attggctctg gtagtgaaaa aaatggaaaa atttgatata
     3001 atctaagagt aaaatttaag caattataaa tgataaactt ttctacatat actttttaat
     3061 caagatatat gtagatttgg ttaaaaagga gctataaatg attaggatat tgttagtgtg
     3121 tgttggaggc atgtcttcta ccctattggt aaataaaatg gagaaggatg caaaaaaaag
     3181 aaatatagac tgtaaaatat gggcagttgg agaaggagat ataaaatcgg agcttgataa
     3241 ctttgatatt ttacttcttg gaccacaact tagatttatg cttgatgacg taaagtctat
     3301 agttggagat agagctccag tatctataat agatatggta aactatggca cttgtaatgg
     3361 tcatgctgtg ttaaattcgg ttttagaaat attaaaataa ataataaaaa tggattctat
     3421 ataatagagt ccatttttat tatttgttat taataggctt aaaacttagc ttaccatttc
     3481 tttttttaat ctccacatag caagtgaaaa acttgaacag cataatatta aaaatagtag
     3541 gataacaggc attatatctc caataagaat agtctcacca aatattaagc tacgtaaagc
     3601 gttaataaca tgggtaaatg gattgagcat cactgctact tttaatcctc cagataaact
     3661 ttctataggg aaaagagctg aacttaaaaa gaaaatagga agtacaattg cagtcataat
     3721 tgtttcataa actatttcat ttggcaataa taaacttata gaataagcta gacttgataa
     3781 aaagaaggct gtcataaata tcagtactat cataagtagg attccaccaa tgccagattc
     3841 tatccttact gagaagaata tacttactat acacaaaata gttacttcta tgaaagaaac
     3901 taagatagct tctaataatt gaccaaggac gattgaatac ctactgatag gtgcaattaa
     3961 gactctatag aaacttccac tgttcttcat tataaagttt ataataccac cactactaca
     4021 agaagaaaac acaaccagta caatgatacc tggtagtata aaagcagtat aattattgat
     4081 atttatatta ttcatagttt ggtttgcaat agaactataa agcacaagcc aaagaaaagg
     4141 ttgtaatata gtaactacaa atgagattgg atttttaaaa cgccacttca tactacgcca
     4201 taagatattt aacattccca ttcctccttt aagctttttt tagaacttgt aagtgctaaa
     4261 aatacatctt cgagacttgg ctctacaatt tctattgcat caaattctat atcatgttct
     4321 aaaagccatt tatttattaa agtaaaagca gttctactat cgtttacact tataaaaatt
     4381 gaattttctc gtacgctcat aaactttact aaaccagtac ttttaattgc atctttatat
     4441 ttttttatat cttcagtatt atggaaagtt atacgtagta tattttgtct aatgtaacta
     4501 cgtaaacttg atggtgttcc ttgtgctaaa tcctttccat tcttcataat acaaatatta
     4561 tcactcaatt gttcagcttc ttcaagataa tgagtagtta gaaatatagt tgtgccatac
     4621 tcatctctaa tttttaataa catatcccac atagattttc ttgaatcgac atccatacca
     4681 acagttggtt catctaaaaa taggatttta ggagatgata ccatattcat agctatgtcc
     4741 aatctacgtt ttacaccccc tgaatatgat gaagtaggat attttaagta actagataag
     4801 tcaaacttat caattaaaga gtctattctt tgttttgcaa tctgtggttc tactttatac
     4861 attttacttt gaaataccat attttccata agggataaat gttcatcaat agagatttgc
     4921 tgtgctacac aagcaatttg agtacgtatc caagaaggat tatctactaa atcttttcca
     4981 aacattgtta catttccaga tgtaggtttg taaaaggtag tcaaaatatt tataagagaa
     5041 gatttaccag caccattagg accaagtaaa gaaaaaattt ctccagcatt tacattaaag
     5101 cttagagcat ttaaagcttt cacaccattt ttatattctt ttactaaatt gtctatttca
     5161 atcgctaaca tatattattc ctcctcttta agtggaaata ttatctctgt aatatagtta
     5221 tctgggtttc cttttatcaa catacctggt cctttaataa atacttctct ccaaggagtt
     5281 tgtattttta tattattttc atgaatatat ttaaatatca tttcatacgt ttttggaaaa
     5341 ttactataag agcctgtgtg agtaaaaaat aaagcttttg ttttaggaaa ttcttttgta
     5401 gttataccca tacgatttgg agtttcagca gttggaacac ataattccat ttctgcaaca
     5461 ccagtttttt ggtcaacatc atagtagcaa aagaatggtg caccgttaga ctttcctttt
     5521 atagctttaa atacatttgg aaaatacttt gttgcctcag tcataggtcc attataacgc
     5581 atggtagcca ctgtgactga ttcgatattt ttgatttcaa ttttgcaatt cattgtttac
     5641 ctcctaatgt gtgttcatat tcttactaga tttttaagca ttaatacata taagattttg
     5701 attagttgaa aacatataat cttatctcgt ttgcttgttt tcaagtctaa cataacataa
     5761 aatatatatc ctctcatttg atgctccatt ttgtaaagtt attatttaag ctttacttta
     5821 gattttttag caaattaaaa taaagcaatt tataaaataa gtatttacaa attaagtgta
     5881 ttaatcgtat aatacactta ataggatact agataaagtc tgaaataaag gcgtattttt
     5941 ttaaagtaag tgtactatcg tattaataca ctagaatagg aggaatcatg aattttgaat
     6001 tagataatac aactcccatt tatttgcaaa tagtaaagta tataaagaga caaatagtaa
     6061 caggagagtt gaaaccagga gagacaatac catcaagaag agaaatggca cttaatctaa
     6121 aagtaaactt aaatacagtt caaagagcat ataaggagat gggtgatatg aatataataa
     6181 atacttttaa aaattatcaa agcagtgtaa cagttgatga aaatatatta aaaaatttaa
     6241 aattagagct tataaatgaa tcactatctg tttttataga agatatgaaa gcaataaatg
     6301 ttagcaaaga agaagtttta aaaataatag aagataagta ttagtttttt aacaaataga
     6361 aaacttagtt tagaaagttt atttataagc atattggatg tggaggatag taaatatgat
     6421 agaaataaag aatgtcagca aaacatataa aaggatgcaa gggcttaaaa taagaaagat
     6481 agaagcttta aaaaatgtaa gttttaatat agaaaaggga aaaataactg cattacttgg
     6541 tataaatggt gttggtaaat caactatgtt aaaagcaata gcaggactta taaacattga
     6601 tagtggagaa attcgtatag atggagaaaa aataaatgaa aaggtataca ataaattggc
     6661 ttttgttcca gatgtacaat ctcactttag caatacaaca ataaaagaaa catttgaatt
     6721 tatggaaatt ttttattcaa agtggaataa agaaaaatca aaagaaatga tggacatatt
     6781 taaattggat gaagatgaaa taattgataa cttgtcaaaa ggaaacattg caagagtaaa
     6841 attaatactt ggattttgcc aagacccaga gtacatactt cttgatgaac catttacagg
     6901 aattgatttg tttaagaggg aagagtttat aggagtaata gcgcaatata tggaggaaaa
     6961 tcaagccatt atcataacta ctcatgagat agtagagata gaaagtttag ttgatgaggt
     7021 tgtaattctt gatgaagggc agattataac ttcatttaat gcagaagaat taagagaaag
     7081 agaaggtaaa tctatattag ataagatgag ggaggtatat aagaatgaat aaacttctat
     7141 atctaataaa tgtagatttg agaagaagca ataagtttta tatagcttat atatcatttt
     7201 tttcactaat tacacttagt ttaaatttat tcgaaattaa taaatttaaa aattctaaat
     7261 ttattatgaa cttagcaata gaagattttg gaggtatatt ttatggacta gggatactta
     7321 gtaatcttgg ttttatccaa agtatattgt tctttgggat attgggactt tttatatact
     7381 atatttttat gtggaaaaga gagttctttt caaataatca aagtatttat acacttatga
     7441 tgttacctca aaataagttt aagatttgta tatctaaatc aatagctttg ataacaatga
     7501 tatatggttt tttgataagt cagacagcaa cattatttat atgtaaatat atatttaatt
     7561 tcatttttag aaatatgcct ataatcaata tgaactttgc caaagattta tattatttaa
     7621 acttgaagag tataccaatt gattttatct cttttatggc tatctatgta tttttactac
     7681 ttatagctat ttctattgta aattgctgta tattatttat cttttcctat ataaataaat
     7741 tttatgtaat tttaatcact ttgctgatta tagcaggctt atatttttca acaatatata
     7801 taaatataat cactttattt agaaaatatt atagtcttat aggatttcaa tttaacaatt
     7861 tagtagcagg gttattaagt tttttaatta taatgtttat tctaaatgga atatcttatt
     7921 tgctaattaa aaagaaagta tctctttaag gaggggtggt atgaaattaa aatctaaaat
     7981 atctatactt attatatttt tgactttagc aatatatttt agtataggtt tattaaataa
     8041 tgaagataat ttaaagataa aaactataaa aggtgactct aatgaattag accatataaa
     8101 cataaatttt ggatatgctt tgaatggata tataaattca tattatacaa taaatggaag
     8161 taacaaaacc aaaaagactg aattaataaa actacaaaag gatagtaaga acacaaataa
     8221 tttaattgat gtatctagtg gttcagattt aattgcacat gttggatttg aaatgaatga
     8281 ggagtcaaaa gatataaact ttgtaataga tgagaaaaaa gaagatttag aaaaaggtgt
     8341 atttccatca gaaggaagtg ataaagaaat tgaatatgaa aaattagata tgaataattt
     8401 tacaaggaca agatataaag tagataaaag tacagatgat tattatgcaa atatactagc
     8461 atcaaaaaga tataaaaaat acttatatgt atctgtactt tatgctacaa gtgattcatc
     8521 agaaaataac aaagaatatt caagtactat agatataata aaaattaatc cagaaaataa
     8581 aaaaatcaag actataaaaa gtataaattt aagtaattat ttttccgaaa aataccaaaa
     8641 catagagacc ataggtgtaa gttatgctag caaatatgag gataagatgt atattttagt
     8701 aaatgtacta aatacaaaca taggtgataa tagtaattct aaatttcatt tacttgagta
     8761 taatcttgaa gaaaataagg aaagcataat ggaattaaaa actgatagca aaatggcttt
     8821 agcaaaagca aagttagaag ctaataaatt atatatggta atgtcgaatg attatgataa
     8881 tagttcaatt acagagttaa atttacttac ctataatata ttagataaaa gcttagatga
     8941 agataaatat gttttagata atagagaaaa aagaggaatt agaagtattg aaattgattc
     9001 aaataaaatc gttgcatata catcaaggct aaactctata ttatcttggg ataataaatt
     9061 tatgctatat ataataaata aaaattctaa ggaaattgta tatgaaggag aattacaatt
     9121 taacagttca aatagtatgt tagaatttga aataaagtag gtgagttgat gaaagattta
     9181 ataagattat tcgaatttga actaaaaaga aatataaaga attatatatt tataataatt
     9241 tgttgttgtt cttttgtaat tttaaacata gtaaaaaatt taaatgacta taattacata
     9301 atagaaaatg cagttaaaag tgaacagtta acaaaaatcg gaaatacgat agaaacggta
     9361 aaggttacag gtttctcaag ctttagaaat ataatgggtt ctacagaatc gtggtttatg
     9421 tttggtataa ttgcatgtat ttgctatgct ttttttattt ggtatagaga ctttaatggt
     9481 agaagtaaat ctatatatac acttataatg ctacctaaaa atagaatcaa tatttatata
     9541 tctaaattat taaatatatt atttctagtg tatagctata cagtagtact tacaatatct
     9601 ttgtttattg caagtaagtt gttgcctaga catatgcttg gtaatgtcac aaactatggt
     9661 tttgtacaag aaacaatata tgagttaaaa atgttacttc catatagctt tgaaatacta
     9721 tttgtagagt atatattttt attgatagga tttgtatctg tagtatttac atcaattctt
     9781 ataaataaat ctatttataa agtaagcact ttaataagtt ttctattttt agttatagaa
     9841 attttagtgt ttataatttc tatagaattt attataccct acgaatacag tgatgtattt
     9901 actgttctat attcaagtat aaacatattt gtatgtagtc ttatatctaa taaacttttg
     9961 aagcaaaaga ttgactttta ggagggatat tatgaaagca aaaaatatag gaaaatatac
    10021 aatatgtttt ataatagtat gttttttaat gattactatg accttaatat tgagttattt
    10081 taataaggat aaactatctg taaaaacttt aaaaggcaat gtaaaagata tagggagagt
    10141 aagtgtagta tatacaccta atataagcaa attttcttat gaggaggtaa ttataagtaa
    10201 aaatggagta agaaataata caaacccaaa atcaagaatt agaaatgatt atttaaatat
    10261 tgacaaaaaa tacagtgact ttgttaatga taaaggaaaa gaatttatat ataaagatga
    10321 tttgaatata ggagaagcgt atacctctgt taagtctgat ttagtacaag gaatgaatgc
    10381 tttaaatgat tgtataaagg tttcaaataa aaatttaaaa agtggggtag aagaaaattt
    10441 tgatattaat ttaaataagt ctgattttaa agaaaatagt tggaaaacct tatttaacaa
    10501 aatatataaa ggtaatttat atatggtaat tagtgaaact aatatttcag gttctaaagt
    10561 caataatatt tgtatactaa agatagatat aaaaaataag tcttataaat tattaaatag
    10621 atttaattta ggattacaga atggagaaca acaaaatatc aatgaaatta aattttctat
    10681 tggaagtaaa ttatacttag aaatagtaaa taataaaagt aaaaatggat atattgaagg
    10741 acatgatagt tactctaact ttattatata tgatatagaa gataacactt ttaatataag
    10801 taataaattt atggataatc taaacaaaga aaagaatgca aatccaaagg agattggctt
    10861 attaagtaaa actaagctag attatatggt aaaaaataat aaattgaatc ttatagttac
    10921 ggatatgagt aattccataa ctaatcttgt ttatgatatt aattctaatg agattaaatt
    10981 agaagattat gagatacttg atttaaaagt agattataat gaagcatcat ctggagttag
    11041 gaaagttaag ctactggatg ataagatata ttctatttca ggtattaaga acagtatatc
    11101 aggagaatta aaaggattta aagggattgt tgatgtagtc ggaactaagc cagtaaaatt
    11161 tcaagtattt gatatgaaac ttaaaaagaa tatttatgaa gctgagctaa taaatggaga
    11221 tataagttta caagaaaagt tattttttgt tgcaaattaa gtgttagaat taattttaga
    11281 actttaaact tttatatgta tcacagataa gtataagtaa gggagtgtct caaaataaac
    11341 tttttagctc atgagacact ttttttatat gaaatcaaat atattttcat tgtttagatt
    11401 tattttgata atgaaaaaaa gattatcttt attttaagaa agacatttat ttataattat
    11461 at
//
LOCUS       DAGSRM010000116        10618 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.120, whole genome shotgun sequence.
ACCESSION   DAGSRM010000116 DAGSRM010000000
VERSION     DAGSRM010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10618
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.120"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            169..546
                     /locus_tag="MJE33_003246"
     CDS             169..546
                     /locus_tag="MJE33_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBY9649162.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDVTVTSKDVIEAMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTIRVKHKDYLSFETKDFLSNIHDDSLRSLISALH
                     DDENIGKDKLDFLESYFDNLKDE"
     gene            539..1687
                     /locus_tag="MJE33_003247"
     CDS             539..1687
                     /locus_tag="MJE33_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HBY9649163.1"
                     /translation="MNNYMLYVFENLIKTTIVCSLGICLLLFLKRYLFKKFSKRFNYY
                     IWLIIVFRMLLFLFNYTIVYEVKEPKENALGNNITQIDISTDNNLMLYVAYLWLFVTI
                     VIAVYTFIKYTRFKNLVVDVSYDIEDNDINCIYKNLLKELNIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYPYSKDELTWILKHELMHFKNKDILIKFLVLSVRIIYWFNPLVYV
                     MSNKVNLDCELCCDESVLVECSLKDKKEYALALIKSIKFSKNYNSKILTTEFDKTNLE
                     KRLESIVKKKGKSGILIAVLVFMTSITSFVEVDAQVRIDEGNNGMNNNINEFLLNKTT
                     DKIINIYYKDAPNEIRKFYEDKCELEGKIPRDYDIIEMDMKDYNQLIK"
     gene            1704..2306
                     /locus_tag="MJE33_003248"
     CDS             1704..2306
                     /locus_tag="MJE33_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HBY9649164.1"
                     /translation="MLTKLRKIVAIMCCLLLTFTSVAFADTSKEKGGSKETVPEKNLD
                     SFTLSGYIYTYENAPQEIRDEHKQNCEEINIDPKPDDEIFVPESANLMNEDNSKGVYA
                     SYTVSYNIGAKTITIMSNEYLTISTTKVIRKGNSGKEVKAAQIMLTLLGYNVGIDSSF
                     GSKTYNAVVSFQKKYGLSADGIIGPATWDKLGRLTDPTLS"
     gene            complement(2399..3421)
                     /locus_tag="MJE33_003249"
     CDS             complement(2399..3421)
                     /locus_tag="MJE33_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBY9649165.1"
                     /translation="MDYNDLLKSARENFNGTCKVCKICNGLACAGDVPGMGGKGSGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTILNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNRGNGIVFIKPWNNSKIIDKI
                     RLSEEAGAFAVGVDLDACGLINNQFQENPFSPKTIDEIRELVESTRLPFIIKGIMTVD
                     DALMAVESGASAIIVSNHGGRVLDYTPGTCEVLPDIAKSVKGKITILVDGGVRTGVDV
                     VKMLGLGADAVLMGRPFVTASFGGGLDGVEFFIEKVRNELCETMILTGCQNVKDIDGR
                     VIWNLL"
     gene            complement(3750..3932)
                     /locus_tag="MJE33_003250"
     CDS             complement(3750..3932)
                     /locus_tag="MJE33_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649166.1"
                     /translation="MYKDKSDECIHLMTAYIDSISGYYSFIDTQLEDFMMKYGENIVD
                     SNLHSIMMLLCKWGLS"
     gene            4245..4664
                     /locus_tag="MJE33_003251"
     CDS             4245..4664
                     /locus_tag="MJE33_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 domain-containing protein"
                     /protein_id="HBY9649167.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYHFYIFSKDGEYVISNISVDYKGL
                     SFNKLSRANKVDNSYIVSVIICENCQDYIVQIENCIV"
     gene            4795..6636
                     /locus_tag="MJE33_003252"
     CDS             4795..6636
                     /locus_tag="MJE33_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBY9649168.1"
                     /translation="MEEIDDTILDSMFKLTLNERLLTNNDFIENKIDVVKNLIDCYKT
                     EQNSLMLLKSNFLLALLYGIQGFSEKMKEYILISCKYIDKCLPKDYKFLARFYSQIAI
                     LSLKNEDVNKANLYMDKFNLICDENNFLIEEIIFKSQLIYIKASKCIESSVIIKEIET
                     LYIKIKEINDFNCKKIYFFIIGKIYFLFLDDALIAKVNFLKARKYADLLKDMQVSSLC
                     NIKIGECECSFENYECSMEYFNEVISNKKYRNVNIIQKYRASNNITKILIKTRNYPVA
                     INNLAKSEVYLEKIKNHSLKEVEKFDLFISLAMYYAKSNEKSFEQSICYLDRAKNILN
                     MIKNIEEYIIYDLEIIYHQAKIYYIFGYYDNALNTNKKLLEKSKNAKNYNYVKLAYKS
                     IYLCFEKIQNYEMSMKYFKMYYELKMMYIKARNRNYIDSLNYRHEYIEKEINNMRKVK
                     LDLDKKKYVDHLTSAYNRTFLNDFLEKQEVSEYCTAFMIDVDYFKEYNDTYGHYNGDI
                     ALKNITIIIKKYLKKDMLMIRYGGEEFLILSVCKDYKKSKIFGRKLCRIVKKFLNNNL
                     TVSIGIDTCKNSSIDIKEIIENADKALYKAKESGRNRCLHYHDFKNF"
     gene            6663..8549
                     /locus_tag="MJE33_003253"
     CDS             6663..8549
                     /locus_tag="MJE33_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBY9649169.1"
                     /translation="MKRSLIEDLKRNINKIEYDMLTKFYLNLTDLNYILDYHKDLLNT
                     KLEDNSRVYTLYLISLINYIKKDREASYNYLIESLKYIKKLSLKDDSDIISKIYIYLT
                     LSAISIRKYDKVNNFYFSAKKIIRQNHLNELLVLLNMSLCVEISALYQTKTDVIALVE
                     EVSFFKTLKNKALVSRANYIFGRVYLYLFNNFIKSMEYLIECLKLAQENNLYSIEAFC
                     KCIISLCYLESGNNVEAIKYINNVLDVAHSNNSISVTERVSIKIDLIWAYLKEDMNKE
                     AEVILLKTIKLMKVVEGVYKDYLYSFIFLYSAEIELKKDNCSFEKVNTYLILSKNIYK
                     SFGDVFIYNSKLDYIYKLYGDLYIKFGNIDEGISCYIKGLNLVKKSSLNLRKISIFYG
                     LIANGYELKKDFKLAIEYYKSMDWYSDRWEKNNSYKTSQAIHKQYELRQKQESLRLLV
                     DDNKKLENDILKDGLTKLYNRRYLEQQLDLFNETRDKNMVAILVDIDCFKSYNDNYGH
                     LAGDKVIVKVTDIIRSVFIDITEHIIRYGGEEILILVDSIESIGVEEIDNIELYIKRV
                     FKLLEFEGIEHIYSKIKNYITISAGVSIKRCRSRKDVEILIEDADKNLYKSKKAGRNQ
                     YIII"
     gene            8557..10557
                     /locus_tag="MJE33_003254"
     CDS             8557..10557
                     /locus_tag="MJE33_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY9649170.1"
                     /translation="MILLRKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLM
                     ENQGRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVV
                     LILGDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEIVIVGGGLACS
                     FARQYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAID
                     SNGSIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEDVEDRKIRNINN
                     LIVNTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKD
                     KLTKEFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAE
                     NLLESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEER
                     QIMRIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRRED
                     IIPLFNNFVNEVLKDDGLNGINSIDENFVLTKDEMDKLYNYSWPGNVRELKTIAQKYV
                     VTGKIKLRQDRDFKTKKLLPNSEVDKFNSETTASVEVQDESINISKINDGKISIDIKE
                     VNKYVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
BASE COUNT         4376 a         1058 c         1653 g         3531 t
ORIGIN      
        1 aaagtaaaat atttaaatag atatatttta aaatctaact tttaatattg taaaaaaatt
       61 agaaatttaa caaaaagtac aaaaattcac atttttttca taaagtgttt gactaaaaaa
      121 ttcacatgtt gtagaattaa ggtagcagga gttttgaggg aggtgtaaat aatgataaga
      181 aaaataccac aagcagaact aaaagtaatg aaattcattt ggaaagtaga tgttacagtg
      241 acatcaaaag atgttattga agctatggaa caaaaatatg gctggaaaca aaccaccaca
      301 ctgacacttt tatctaggct ggtaaaaaga ggattcttag atgctcaaaa aatagacaga
      361 tatacacatt acacaataag agtaaaacac aaagattatt taagttttga gacaaaggat
      421 ttcctaagta atatacatga tgactcttta agaagcttaa tttcagcatt acatgatgat
      481 gaaaatatag gtaaagataa gctagatttt ctagaaagtt attttgataa tttaaaggat
      541 gaataattat atgctatatg tttttgaaaa tcttataaag acaactattg tatgtagtct
      601 tggtatttgt ctattattat ttttgaaaag atatttattt aagaagttta gtaagagatt
      661 caattattat atttggttga taatagtctt tagaatgtta ctttttctat ttaattatac
      721 aatagtgtat gaagtaaaag aacccaaaga aaatgcatta ggaaataata ttacacaaat
      781 tgatatttct acagataata atcttatgct gtatgtagct tatttatggt tatttgtaac
      841 tatagtcatt gcagtttata catttataaa gtatacaaga tttaaaaatc tagttgttga
      901 tgtatcttat gatattgaag ataatgatat aaactgtatc tacaaaaact tgttaaaaga
      961 attaaatata aaaaagaaaa ttgaattaag aggttctgat gaattgataa gtccagcagg
     1021 tatgggatta tttaaatctt acatattctt accagactat ccttatagta aagatgaact
     1081 aacttggatt ttgaaacatg aattaatgca ttttaaaaat aaagatattt taataaagtt
     1141 tttagttttg tctgttagaa taatatattg gtttaatcca cttgtatatg taatgagcaa
     1201 taaagtaaat ctagattgtg aattatgttg tgatgaaagt gttttggtag aatgttcttt
     1261 aaaagataaa aaagaatatg cactagcact tataaagtca attaagttta gtaaaaatta
     1321 taatagtaag attttgacaa ccgagtttga taagacaaat ttggaaaaga gacttgagag
     1381 tattgttaaa aagaaaggca aaagtggaat actaattgct gttttagtat ttatgacttc
     1441 tataacatct tttgttgaag ttgatgctca agttagaatt gatgagggta ataatggaat
     1501 gaataacaat attaatgaat ttttattgaa taagacgact gataaaatta taaatattta
     1561 ttataaagat gcaccaaatg aaattaggaa attttatgaa gataaatgcg aattagaagg
     1621 taaaatacct agagactatg acataataga aatggatatg aaagattata atcaattaat
     1681 aaaataatta taaggaggat tttatgttaa caaaattaag aaaaatagta gctattatgt
     1741 gttgcttatt gcttactttt acaagtgtgg catttgctga cacaagtaaa gagaaaggag
     1801 gaagtaaaga gactgttcca gagaaaaacc tagattcttt tactctttct gggtatatat
     1861 atacatatga aaatgcgcca caagaaataa gagatgaaca taaacaaaat tgtgaagaga
     1921 taaatataga ccctaagcca gatgatgaga tatttgttcc agaatcagct aatctaatga
     1981 atgaggacaa tagtaaaggt gtttatgcaa gttatactgt ttcttataat ataggtgcta
     2041 aaactataac aattatgagt aatgagtatt tgactataag tacaacaaaa gttatacgaa
     2101 agggtaatag cggaaaagaa gtaaaagctg cacaaatcat gttaacttta cttggttata
     2161 atgtaggtat agatagttct tttggaagta agacatataa tgcagttgta agtttccaaa
     2221 aaaaatacgg tttgtctgca gatggaataa ttggtccagc tacttgggat aaattgggta
     2281 gactaactga ccctactctt tcataggatt aaaatctaaa aatatattaa atagtgtctc
     2341 aatatgattt ttcaatttaa atgagacact atttaaatat aaatgttata aaactatttt
     2401 acaataaatt ccatataact ctaccatcta tatccttaac attttgacaa ccagtcaaaa
     2461 tcatagtctc acataactca ttcctaacct tctctataaa aaactccaca ccatcaagac
     2521 caccgccaaa agaagcagta acaaaaggtc ttcccatcaa aacagcatca gcacccaatc
     2581 caagcatctt aactacatca acaccagtcc taactccacc atcaacaagt attgttatct
     2641 ttccctttac agacttagct atatctggaa gtacctcaca agtaccagga gtataatcaa
     2701 gtactcttcc accatgattt gaaacaataa tggcactagc accagactct acagccatca
     2761 aagcatcatc tacagtcata attcctttta taataaaagg taatctagtg gattcaacca
     2821 attctctaat ctcatcaatt gttttaggag aaaaaggatt ttcttgaaat tgattattaa
     2881 taagtccaca agcatcaagg tctacaccaa ctgcaaaagc acctgcttct tcagacaatc
     2941 taattttatc tattatcttt gaattattcc aaggctttat aaatacaatt ccatttcccc
     3001 tattatcctt caatacatct aaattatcca gtaaaaaagt atctacatta gtatcgccaa
     3061 ccatagcata aattccagaa ttagaacaac ctctaacaac aggctctatg tattcttttt
     3121 cggaaacttt accacccatg ttcaatatag tacctgatac aggtgcagcg aatataggag
     3181 aactcatttt tctaccaaat aattctatgg atgtatctgg ctttgaaaca ttatgtatga
     3241 ctctcatatt tatctttatt ttttctagac tttttctatt ttcaatgaaa gacgaaccac
     3301 ttcctttacc acccatacca ggaacatctc cagcacaagc taatccatta caaattttac
     3361 atactttaca tgttccatta aagttctctc tagctgattt tagtaagtca ttgtaatcca
     3421 tacaaaatct ctccttttta taatattaaa attgagtatt actatattga aaatatctta
     3481 attgtaaata tagggatgtt attatgatga gctaaactag ataatacata ttagatattt
     3541 tttattgttg tagatagtta aattatatca tattagatag tcaaattttg aaaaaaacta
     3601 acttatttaa gtatacattt tgaaaaaatt aaaaaagcta tatatagatt tttaaagaaa
     3661 tctatatata gcttttaagt ttatgtcaat cttttttgtt aatgaataaa taatatagaa
     3721 tacaatatac aaaaaagttt cttgaaatgc taagataatc cccatttaca aagcaacatc
     3781 attatactgt gtaaatttga atcaacaatg ttttcaccat atttcatcat aaaatcctct
     3841 aattgtgtgt caataaatga ataatatcca cttatactat cgatgtatgc agtcattaag
     3901 tgtatacatt catcactttt atctttatac atatatatct ccccctatat acaatatata
     3961 aatttagata ccaaatcgta tcatattaaa tagttaagtg tgtaacatct atgcagttat
     4021 atatttatat cattatatta ataactaaag tgagttttga caatagattt tgggaaactg
     4081 gatgttcaaa gtgcaaactt ggattttaaa atcattaatt aagatagcgc ataggaatat
     4141 atgtatacat aacttaatta taaagattat aaacaattac ttttattaac tttatttaaa
     4201 tggtagaata acttggagat taataattat aaggaggata taatatgagt ttatataagt
     4261 ttatatatga tgataaggaa tatttattaa aagaggagaa ctgtagtgct ttaataaatg
     4321 atgaagaaaa tccagttcaa ggagtaagta tttcaaaaat aattgatata cttaatgaag
     4381 cagaagaagt tgattttgat gtagaatatt atcaagaagc atgtccatta tgtttagaag
     4441 gagtaaaaga aaagaaaaaa ttctttcctt ttttagaata tcacttttat attttcagta
     4501 aagatggaga atatgtaata agcaatatat ctgttgatta taaaggacta tcttttaata
     4561 aactatcaag ggcaaataaa gttgataata gttatattgt aagtgtgata atttgcgaaa
     4621 attgtcagga ttatatagtt cagatagaaa attgtatagt ttagtgtaaa gtatgtctta
     4681 atttaagaga aatttctaaa ttgagacatc aacttttttc aaaaatagta tggaaaatta
     4741 ctaaatttat gagataatgt aagtcggagg tgagttacaa tatatagtaa atatttggaa
     4801 gagatagatg atacaatttt agattcaatg tttaaactaa ccttgaatga aagattattg
     4861 actaataacg attttattga aaataaaatt gatgtagtaa agaatttaat tgattgctat
     4921 aaaactgaac aaaatagttt aatgttatta aaatcaaatt ttttgctagc tttattatat
     4981 ggtattcaag ggtttagtga aaaaatgaaa gagtatattt tgatttcctg taaatatatt
     5041 gataaatgct tacccaaaga ttataaattt ttagcacgat tttattcaca aatagctatt
     5101 ttaagtttaa aaaatgaaga tgtaaataag gcaaatttat atatggataa atttaatctt
     5161 atttgtgatg aaaataattt tctaattgaa gaaattattt ttaaatcaca acttatttac
     5221 attaaggcaa gtaagtgtat agaatcttct gtaataatta aagagataga aacgttgtat
     5281 ataaagataa aagaaattaa cgattttaat tgtaaaaaaa tatatttttt tattattggt
     5341 aaaatatatt ttttgttttt ggatgatgct ctaattgcta aggtaaattt tttaaaagca
     5401 agaaaatatg cagatttact aaaggatatg caagtatctt cactatgtaa tataaagata
     5461 ggtgagtgtg aatgttcgtt tgaaaattat gaatgttcca tggaatattt caatgaagta
     5521 ataagcaata aaaagtatag aaatgtaaat ataattcaaa aatatagagc atcaaacaac
     5581 ataactaaaa tattaataaa gactagaaat tatcctgtcg caataaataa tttagcaaaa
     5641 tctgaagtat atctagaaaa aattaaaaat catagcttaa aagaagtaga gaagtttgat
     5701 ttatttataa gtttagccat gtattatgca aaaagtaatg aaaaatcttt tgaacaatca
     5761 atatgctatt tagatagagc taaaaacata ttaaatatga taaaaaatat agaagaatat
     5821 ataatatatg atttagaaat aatttatcac caggcaaaga tatattatat ctttggatat
     5881 tatgataatg ccttaaacac aaataaaaaa ctccttgaaa aatctaaaaa tgctaaaaat
     5941 tataattatg tgaagttagc atacaagagt atatatttgt gttttgaaaa gattcaaaat
     6001 tatgaaatga gtatgaaata ctttaaaatg tattatgagt taaaaatgat gtatataaaa
     6061 gctcgaaata gaaattatat agattcctta aattatagac atgagtatat agaaaaagaa
     6121 ataaataaca tgagaaaagt aaaacttgat ttagataaaa aaaagtatgt tgaccatttg
     6181 acaagtgcat ataatagaac ttttttgaac gattttttag aaaaacaaga agttagtgaa
     6241 tattgcacag catttatgat agatgtagat tactttaaag aatacaatga tacttatgga
     6301 cactataatg gagatattgc tcttaaaaat ataactataa taataaaaaa atatctaaaa
     6361 aaagacatgc ttatgataag gtatggtgga gaagaatttc tgatattatc tgtatgtaaa
     6421 gattataaaa aaagtaaaat atttggaaga aaattatgta ggattgtaaa gaaattttta
     6481 aacaataatt tgacagttag tattggcatt gatacatgta aaaatagtag tatagatata
     6541 aaagaaataa ttgaaaatgc tgataaagct ttatataaag caaaggaaag tggtagaaat
     6601 agatgtttgc attatcatga ttttaagaat ttttaactgt tgaaatagga ttggtgattt
     6661 acttgaaaag gagtttaata gaagatttaa agagaaatat aaacaagata gaatacgata
     6721 tgttgactaa attctatcta aatttgacag atttaaacta catcttggat tatcacaaag
     6781 accttttaaa tacaaaatta gaagataata gtagagtcta tactttatat ttaattagtc
     6841 ttataaacta tataaaaaaa gatagagaag catcatacaa ctatttgata gagtcattaa
     6901 agtatattaa aaaattgtca ttaaaagatg attcagatat aatatctaaa atttatattt
     6961 atcttacttt atcagctatt tctataagaa aatatgataa ggtaaataat ttttatttct
     7021 cagcaaaaaa aattataaga caaaatcatt taaatgagtt gttggtattg ttaaatatga
     7081 gtttatgtgt agaaataagt gctttatatc agaccaaaac ggatgtaata gctttagtag
     7141 aagaagtgtc tttttttaaa actttaaaaa ataaagctct tgtaagtagg gcaaattata
     7201 tatttgggag agtttatcta tatttattta ataactttat aaaaagtatg gaatacttaa
     7261 ttgaatgtct aaaattggct caagaaaata atttatactc tatagaggct ttttgtaaat
     7321 gtataataag tttgtgttat ttagagagtg gaaataatgt agaagctata aaatacataa
     7381 ataatgtatt agatgttgca cacagtaata actcaatatc agtaactgaa agagtgtcta
     7441 taaaaataga ccttatatgg gcatatttaa aagaagatat gaataaagaa gcagaagtta
     7501 ttttattaaa gactataaaa ttaatgaaag ttgttgaagg tgtatataag gattatttat
     7561 attcatttat atttttatat tcagctgaaa ttgagttaaa gaaagataat tgtagttttg
     7621 aaaaggtaaa cacctattta attttatcaa aaaatatata taaaagtttt ggagatgttt
     7681 ttatatataa ttctaaacta gattacatat ataagttgta tggagattta tatataaaat
     7741 ttggtaatat agatgaaggt ataagttgtt atataaaagg tttaaattta gttaaaaaga
     7801 gttcattgaa tcttagaaaa atatctatat tttatgggct aatagcaaat ggatatgagc
     7861 taaaaaaaga ttttaaactg gcaatagaat attataaaag tatggattgg tattctgata
     7921 ggtgggaaaa aaataattcc tataaaacat ctcaagcaat tcacaaacaa tatgaactca
     7981 gacaaaaaca agagagttta agattactag tggatgataa taagaaatta gaaaatgata
     8041 ttttaaaaga tggacttact aaactatata atagaagata tctggaacag cagttggatt
     8101 tatttaatga aaccagagac aaaaatatgg tcgcaatact ggtcgatata gattgtttta
     8161 aaagttataa tgataattat ggtcatctag caggagataa agttatagtt aaggtaactg
     8221 atattataag aagtgtattt atagatatta ccgaacacat aatcagatat gggggagaag
     8281 aaattttaat attagtagat tctatagaat caatcggtgt tgaagagatt gataatatag
     8341 aattatatat taaaagagtt ttcaaacttc ttgaatttga gggtattgaa catatatatt
     8401 ctaaaataaa aaactatata actataagtg ctggagtaag tataaaaaga tgtcgtagta
     8461 gaaaagatgt tgaaatatta attgaagatg cagataagaa cctatataaa tcaaaaaaag
     8521 caggtagaaa tcaatacatt ataatttaaa attgaggtga ttttgttgcg aaaaaaaata
     8581 ggaattatag cctctgatat tgaattaaaa gaaagaatag aagaattata tagagaagat
     8641 gtagaaaatg gaactataat aatcgacata cttaacctag acttaatgga aaatcaaggt
     8701 cgtatacttg ttgaaaaagg tgcccaagct ataattggta gaggtggagg ttatagttta
     8761 gtaatagata cagtaaatgt acctgttata ccaatgaata tgaagtcaac tgacctttta
     8821 agagcaatag aaatagcaaa gaaatattct aaaaaagtag tattgattct tggagacaat
     8881 gaagtttctt ttgactatgt tggctggaga aatgtcataa gtactgaaat aactgaagaa
     8941 tggtttgaat ctaagtatga gataagaagt aaagttgtaa aatatataga ccaaaaagat
     9001 gaaattgtaa tcgtaggagg aggtttggca tgttcttttg caaggcaata tggaattgat
     9061 agtgtatttg caactgctag tgatgaatct ataagagaag ctgtagaata ttgtaaaaaa
     9121 ttattagata cattaggtga agaaaagttt aataatgaag tgttacgaaa tatacttgat
     9181 ggtataaaag atggagtaat tgctatagat agtaatggca gtataatttt gtacaatgaa
     9241 agtgcaaaaa acatgcttaa agtagagaga aaatgtgcac ttaataaata catcttagat
     9301 gtatttccta aaatggagtg gatgttagat tgtttacatg aaaaagagga cgtagaagat
     9361 agaaagataa gaaatataaa taatctaatt gttaatacta ggacaacatt gataaaagtt
     9421 gataatagta cttatggagt tttaggaata atacaagata taaccaagct acaaaactta
     9481 gaaagaaaaa taagatttga cttgaatcaa aaaggtctct atgctagata tacttttgat
     9541 gattttttat ttaaagataa attaactaaa gaatttattg aagaagctaa aaaaattgga
     9601 aaaagtgatt atacaacttt actttatggg gaaagtgggt ctggtaaaga aattatagct
     9661 catagtatac acaatataag taaaagaaaa gataggcctt ttgttgctat aaattgtgct
     9721 actatagcag aaaatttact tgaaagtgaa ctttttggct atgaagaagg tgcatttact
     9781 ggtgctagaa agggtggaaa aagagggcta tttgaacttg ctcatggagg gactcttttt
     9841 ttagatgaaa taaatagtct atcctttaat attcagacaa aactattaag agtaatagag
     9901 gaacgtcaaa taatgagaat tggttctgat tatataatac ctctggatat aaggattata
     9961 gcagcaacga acgaatcttt aaccgaaaaa atagttatgg gtacatttag agcagactta
    10021 ttttatagat tgagtagttt agagataaat atacctcctt taagagacag aagagaagat
    10081 ataatacctt tgtttaacaa ttttgtaaat gaagtattaa aggatgatgg tctcaatgga
    10141 ataaacagta tagatgaaaa ttttgttttg actaaagatg aaatggataa gctatataat
    10201 tatagctggc ctggaaatgt aagagagtta aagacaatag ctcaaaaata tgttgtgaca
    10261 ggtaaaatta aattgagaca agatagagat tttaaaacta aaaaattact tcctaactct
    10321 gaagtagaca aatttaattc tgaaacgaca gcaagtgtag aagtccaaga tgaatctata
    10381 aatataagta agataaatga tggtaagata agtatagata taaaagaagt aaataagtat
    10441 gttgaagaaa aaataataag tatgcttttt gctcaaggac tttctaaaaa tgaagttgca
    10501 caagttcttg gaataagcag gacatcttta tggaagaaat acaataaaaa tatttaaata
    10561 taattacata taaattattt tgaagtaatg ttaatttttt aaaacaagtg ttaagaaa
//
LOCUS       DAGSRM010000117        10446 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.121, whole genome shotgun sequence.
ACCESSION   DAGSRM010000117 DAGSRM010000000
VERSION     DAGSRM010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10446
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.121"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            <1..961
                     /locus_tag="MJE33_003255"
     CDS             <1..961
                     /locus_tag="MJE33_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBY9649171.1"
                     /translation="ILIFLLCNYTKTFNSIYFVPLILDISFLIKEKYKYILFSFVIIS
                     SLVISLDKNIYLALESSSILLIITVLSIYIENENLSKLYSQNLYDKLRISKDELKKVN
                     ADLETYASSIEELAILKERNRISREIHDSVGHSLSTTIIQLNAIEKLLKDKPLIYDLV
                     HELREFVTESFQDVRRAISELKPVEYENYQSLFKIKELVKSFIKLTNINVKLTISKNT
                     WNLSRNQSITLYRLIQESLSNSSRHGKATEIRIFITFNTSNLIITISDNGIGCGNIKK
                     GNGLNSINERIYELNGKVEFDNSNNGFTIRASFPKFSGGDFFE"
     gene            954..1601
                     /locus_tag="MJE33_003256"
     CDS             954..1601
                     /locus_tag="MJE33_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY9649172.1"
                     /translation="MNKIKVLIVDDEKLIRKGLKIILSSYNDLEIVGDASNGYEALEF
                     CKMNDVDIVLMDIRMKVCDGVLGTRLIKEYNNSITLLILTTFNDDEYIKDAMKFGASG
                     YLLKDSSDEVLHEGIRSSFFGNIVLDKSVAEKIMTSEKTVKQEHLYDMYNLTEKEISI
                     IRLIANGLNNKEISQELFLSEGTIKNNITNILAKLELRDRTQLAIFAFKNKIVIE"
     gene            1730..2665
                     /locus_tag="MJE33_003257"
     CDS             1730..2665
                     /locus_tag="MJE33_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9649173.1"
                     /translation="MDIVRVNNITKRFNDKLVLDNISFSVKKGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGSIQINGLDLSKDYIKAKSIIGLVPQELAIIETLTSFDNLEYFGA
                     FYGLKGKLLKERIIEALEVTGLTEVKKKKVKKLSGGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPQSRNHIFNFIKDISKKHETTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGN
                     NDYLKSLVSTNTKLIMEIKNINAQLIFDLKNTKGVISVLENNSLLELDIDKKLQLTDI
                     LSVIDKNNSKIMKISYEEPSLEDVFLNLTGKNLRD"
     gene            2679..3776
                     /locus_tag="MJE33_003258"
     CDS             2679..3776
                     /locus_tag="MJE33_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9649174.1"
                     /translation="MKLYMSVKTMLKSLKSSFILNLIYFLALPLILSWFLGMVTESMF
                     QNPIKTESTPIVIYDKDNTRLSNDLTKYLKNDLSYILTVKKDDSKAELKLTIPKGYES
                     SLLNEKSNTLNIEKLGTRDDIAILLQDILDTYHEKFYLNNSQKISSEDFSKLFNKNSI
                     DTSIINNNVKQSSYEYFALVSLGFLVIIFIMNNILSNYISESKGLSKRLYSMPITRVQ
                     FLIYDFVGLWIYSFIFLLLYVLFFRILGITFKGNFAILLLLCALSSYFMTSISTFVTS
                     FFSKKYGTAIVYALLFLQTIFGGIFSMISDTFTKLTSLSPTYLIGELFSNYETFKTID
                     SIGDLIFTCLIISTILIALAIVKENYKWREV"
     gene            3779..4843
                     /locus_tag="MJE33_003259"
     CDS             3779..4843
                     /locus_tag="MJE33_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9649175.1"
                     /translation="MIRLTIANIKRYLKNHTLLINMVMLPIILIFSLNFFINNSGNQS
                     MYFSPVAIVSDSSGKYEHKLINSSKLKENSFRLNEQDKAMNLLKNNKVSAVFVLDKNF
                     SNSIDKLQRPVVKCFKIENGGGSLWAESQIESFITKSLKLKVDKNIDDKLTKTNIIDN
                     KPDKNKGSFLVVFLICYFMYINAAHLASDLFTLKKSNVLKRLISTDNKDIKIIFSIFL
                     GLFFIQSIVYTFALLCFSIIEDFNLSLNVLMIVLANSFVSTGFVFWVARVFKNESSIS
                     LISTFYSLIGLAISISSLIPSMDKLSFMTNLSKFTPFYWTIDAIKNNGSILINIIALI
                     LIGIIFVTAGSLKLRDFAKN"
     gene            5087..5584
                     /locus_tag="MJE33_003260"
     CDS             5087..5584
                     /locus_tag="MJE33_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBY9649176.1"
                     /translation="MYKIGESVMYPKEGACSVNDIVTKKINHEMQKYYELSVIFNSNL
                     KISIPVLNADRIGIRPVMDGNDVDNFIQSINKTDGVWIFDRKERLKLYQDKFHSGDVF
                     EIVKLIKMLMIQDSSKQLCSTDKEFLNKAQKFALSELAAAQCKSYTMVLEEMKKHILN
                     SKNIN"
     gene            complement(5761..6009)
                     /locus_tag="MJE33_003261"
     CDS             complement(5761..6009)
                     /locus_tag="MJE33_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649177.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELSVLLQEKKELEED"
     gene            complement(6075..7091)
                     /locus_tag="MJE33_003262"
     CDS             complement(6075..7091)
                     /locus_tag="MJE33_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBY9649178.1"
                     /translation="MEYNELLKSARENLNGSCKVCKVCNGVACAGEVPGMGGKGSGSS
                     FIENSKSLEKVKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCANSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVKQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVDAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSSSMILTGCQTIKDIDGK
                     VIYK"
     gene            complement(7149..7916)
                     /locus_tag="MJE33_003263"
     CDS             complement(7149..7916)
                     /locus_tag="MJE33_003263"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBY9649179.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRVDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENEGYDKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDISQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYEAIEKYGITPIHRKSFLKNIL"
     gene            complement(7984..10173)
                     /locus_tag="MJE33_003264"
     CDS             complement(7984..10173)
                     /locus_tag="MJE33_003264"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HBY9649180.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRELAYDISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKRFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEDFIDIKTDLEKVTKANISVRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNFMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKEDKKENSKKEYYKNNKKEKDN
                     KDEKAKEIINKNFEEDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
BASE COUNT         3634 a         1595 c         1199 g         4018 t
ORIGIN      
        1 tatattaatt tttttacttt gtaattatac aaaaactttt aattcgattt actttgttcc
       61 tttaatatta gatatatcat ttttaataaa ggaaaaatat aagtacatat tgttctcttt
      121 tgttataata tcttctcttg ttattagttt agataaaaac atatatttgg cactggagtc
      181 aagctctata ttgctaatta taactgtact ttctatatat atagaaaatg agaatttatc
      241 aaaattatac agtcaaaatc tttatgataa gcttagaatt tctaaagatg aattaaaaaa
      301 agttaatgct gatttggaaa cttatgcaag ttctatagaa gaattagcaa ttttaaaaga
      361 aagaaatcga atatcaagag aaatacatga tagtgtagga cattctcttt ctacaacaat
      421 aatacaatta aatgctatag aaaaactctt aaaagacaaa cctctaattt atgacttagt
      481 acatgaactt agagaatttg taactgaaag ttttcaagat gtaagaaggg ctatatctga
      541 gttaaaacct gttgaatatg aaaattacca aagtttattt aaaatcaaag aattagttaa
      601 aagctttatt aagcttacaa atattaatgt taaattaact atttcaaaaa atacttggaa
      661 cttatccaga aatcaatcta taactttata tagacttata caggaatccc tttccaactc
      721 atctaggcat ggaaaggcaa ctgaaatcag aatttttatt acattcaata cttcaaatct
      781 tataattact attagtgata atggtatagg atgtggcaat ataaaaaaag gaaatggact
      841 taatagtatc aatgagagaa tatatgaatt aaatggtaaa gttgaatttg ataattctaa
      901 taatggtttt acaattagag cttcttttcc taaattttca gggggtgatt tttttgaata
      961 aaattaaagt tcttatagta gatgacgaaa aattaattag aaaaggtctt aaaattatat
     1021 tatcatctta taatgattta gaaattgtag gagatgcttc taatggatat gaagctttag
     1081 aattttgtaa aatgaatgat gtagacatag ttcttatgga tatacgaatg aaagtatgtg
     1141 atggggttct aggtacaagg cttataaagg aatataataa ttctataaca ttattaattt
     1201 taacaacttt taatgatgat gaatatataa aagatgctat gaagtttggt gcttcaggat
     1261 accttttaaa agatagctct gatgaagtat tgcatgaagg gattcgttct tcattttttg
     1321 gcaatattgt gcttgataaa agtgtagctg aaaaaatcat gacctctgaa aaaacggtta
     1381 aacaagagca tctttatgac atgtacaatt taacagaaaa agaaatatct ataattagac
     1441 taattgcaaa tggacttaat aataaagaaa taagtcaaga actattctta tctgaaggta
     1501 caataaagaa taacataact aatattttag ctaaactaga acttcgtgac agaactcaac
     1561 tagcaatctt tgctttcaaa aataaaattg taatagaata aatctaaaaa tataaagtga
     1621 cttaagtcac tttttttagt gacttatagt aatagaatta taattcacaa ttctttatta
     1681 taatattata gctttttaac ttatacaaaa ttattttgga gggattacta tggatatagt
     1741 cagagtaaat aatataacaa aaagatttaa tgataaatta gtacttgata acatcagctt
     1801 ttctgttaaa aaaggtgaga tatttggact tattggccca aatggtgcag gtaaatctac
     1861 acttataaat ataattacaa atcttatgtt accaaatagt ggtagtattc aaatcaatgg
     1921 tttagattta tcaaaagatt atataaaagc aaagtctata atagggttag ttccacaaga
     1981 attagcaatt atagaaactc ttacttcttt tgataactta gagtactttg gagctttcta
     2041 cggtttaaag ggaaaactac ttaaagaaag aatcattgaa gcattagaag ttacaggtct
     2101 tactgaagtc aaaaagaaaa aggtcaaaaa attatctggt ggtatgcaaa ggagattaaa
     2161 tattggaata gcactcttaa atcatcctaa aatattaata ttggatgaac caactgtagg
     2221 tgtagacccc caatcaagaa atcatatttt caattttata aaagatatat ctaaaaaaca
     2281 tgaaactact gtaatatata cttcccatta tatggaagaa gttgagcacc tttgttctaa
     2341 aatttttatt atggatgaag gtaaagaaat tgcttttgga aataatgatt atttaaaatc
     2401 actcgtatct acaaatacaa aattaattat ggaaataaaa aatataaatg cccaacttat
     2461 ctttgattta aaaaatacta aaggtgttat atctgtttta gaaaataatt cattattaga
     2521 attggatata gataaaaaat tacaacttac agatatactc tctgtcattg acaaaaacaa
     2581 ttcaaaaata atgaaaattt cttatgaaga acctagccta gaagacgtat ttttaaatct
     2641 aacaggaaaa aatttaaggg actaatggag gtgattttat gaaattatac atgagtgtaa
     2701 aaactatgct aaagagttta aaaagctcct ttatacttaa tttaatatat tttttagctt
     2761 tgcctctgat tttatcttgg tttcttggga tggttacaga aagcatgttt caaaacccta
     2821 taaaaacaga atctaccccc attgttatat atgacaaaga taacacaaga ttatcaaatg
     2881 atttgacaaa gtatttgaaa aatgatttat cttatatatt aacagttaaa aaagatgatt
     2941 ctaaggcaga attaaagctt actataccta aaggctatga gagtagttta ttaaatgaaa
     3001 aatcaaatac tctaaacata gaaaaacttg gaactcgtga tgatatcgca atattgcttc
     3061 aagatatatt agatacttac catgaaaaat tttatttaaa taattctcag aaaataagtt
     3121 cagaagattt ttctaaatta tttaataaaa attctattga tacttctata ataaataaca
     3181 atgttaaaca gagctcttat gaatactttg ctttagtttc actaggattt cttgtaataa
     3241 tttttataat gaacaatatt ttgtcaaatt atataagtga atcaaaggga ctttcaaaaa
     3301 gactttattc tatgcctata actagagtac aattcttaat ttatgacttt gtaggtttat
     3361 ggatttattc atttatattc ttattattat atgttttatt ctttagaata cttggaataa
     3421 catttaaagg taattttgcc atattgctcc tactttgtgc tttatcatct tattttatga
     3481 cttcaatatc tacctttgta accagctttt ttagcaaaaa atacggtaca gcaatagttt
     3541 atgcattact ctttttacag acaatttttg gaggtatctt cagtatgata agtgatacat
     3601 ttactaagct tacaagttta tctcctacct atttaatagg agaattgttt agtaattatg
     3661 aaacatttaa aactatagat tcaataggag atttaatttt cacatgttta attatctcta
     3721 caatattaat tgcacttgct attgtaaagg aaaattataa atggagggag gtttaataat
     3781 gattagatta actattgcaa atataaaaag atatctaaaa aatcatactc ttttaattaa
     3841 tatggttatg cttcctatta tactaatttt ttcacttaat ttttttatca acaactcggg
     3901 aaatcaatca atgtattttt ctccagtagc aattgtatct gactcttcag gaaagtatga
     3961 acataagtta ataaatagtt ctaaattaaa agaaaattca tttagattaa atgaacaaga
     4021 taaggctatg aatctattaa aaaataataa ggtttctgct gtattcgttt tagataaaaa
     4081 tttttcaaat agtatagata aattacaacg acctgtagtt aaatgcttta aaattgaaaa
     4141 tggtggtggt agtctttggg cagaatcaca aatagaatct tttattacta aatcgttaaa
     4201 attgaaagtg gataaaaata tagatgataa attgacaaag acaaatataa ttgataacaa
     4261 accagataag aataaaggtt catttttagt agtcttcttg atatgctatt ttatgtatat
     4321 taatgcggca catcttgctt ctgatttatt tacattaaaa aaatcaaatg tgctaaaaag
     4381 acttatctct acagataata aagatataaa aataattttc agtatatttt taggactatt
     4441 ttttattcaa tcaattgtat acacttttgc tctattatgt ttttcaatta tagaagactt
     4501 taatttgagt ctgaatgtat tgatgattgt actagcaaat agttttgttt ccactggatt
     4561 tgtattttgg gtagctagag tatttaaaaa tgaaagttct ataagtctta tatctacatt
     4621 ttattcttta ataggtctag ctatatctat ctctagtcta ataccatcaa tggataaatt
     4681 atcctttatg actaatcttt caaagttcac accattttat tggactatag atgctattaa
     4741 aaacaatggt agtattttaa taaatataat agctctaata ttaattggaa ttatatttgt
     4801 aactgcagga agtcttaaac taagagattt tgctaaaaac taataattat taaatataat
     4861 tagtatctat ttttatataa ttgtaaaaga atatttcaac atattaaatt attttatctt
     4921 atatttttct ctttagtttc acacatcacc aagtctaaac tttatattat atattaatat
     4981 ttttgacttg tcaagttatt tatgttataa tattaatcat tagataatta aagcattgta
     5041 cctaagttgc aatatctaaa attcaatttg gaggtataac tagcatatgt ataaaattgg
     5101 tgaaagtgta atgtatccaa aagagggagc ctgctctgta aatgatattg taacaaagaa
     5161 aataaatcat gagatgcaaa aatactatga attaagtgtc atttttaata gtaaccttaa
     5221 aatttcgatt ccagttctaa atgcagatag aattggcata cgtccagtta tggatggaaa
     5281 tgatgttgat aattttatcc aatctattaa taaaacagat ggtgtatgga tttttgatag
     5341 aaaagaaaga cttaaactgt accaagataa atttcattca ggagatgttt ttgaaattgt
     5401 aaaattaatc aaaatgctta tgatacaaga cagttcaaaa caattgtgta gcacagataa
     5461 ggagttttta aataaagctc aaaaatttgc tttatcagaa ttagctgctg ctcaatgtaa
     5521 atcctataca atggtattag aggaaatgaa aaaacatata ttaaattcaa agaatataaa
     5581 ttaaaccagt tttttaaact ggttttattt tttatactaa tcatccatgc tactttagct
     5641 tattttaaac tgcctaatct aatattaata aagttatctc aattagatag atgtatttta
     5701 ttcaaataaa aaaaaactaa gagtaaatca tattactgat ttactcttag ctaataaaat
     5761 ttaatcttct tcaagttctt ttttctcttg taataataca cttaattcat ttgcttctaa
     5821 aacttgtgcc tctgcaatat caagtaactc ttgttgccat tccacacatt tatctttgta
     5881 atcaaaaggt agcgttctgt caactatgta cttatcaata cagtcctccg cacttttaat
     5941 tatttctttt ctttcatcaa taaatttttc taattctact aaggcttctt cataagcttt
     6001 tctactcata taaattcccc ctttgtacaa ttattctaca ttaaaatgat ttttcctctt
     6061 ttttgtataa aattttattt gtatataact tttccatcaa tatcttttat agtttgacat
     6121 ccagttaata tcatagaact acttagctct gattgaagtt tatttacata agtttctaca
     6181 ccatcagttg caccaccaaa agaagcagtc acaaaaggtc ttcctattaa tactgcatct
     6241 gcacctaaac ctatcatttt tagtacgtct aaaccagttc tcactccacc atctgcaagt
     6301 attgtaactt tacctttaac agcatcagca atttttggta aaacttcaca tgctcctggt
     6361 gtacagtcaa gcactcttcc tccatgattt gatacgacta ttgcatccac acctgcttct
     6421 actgccatta aagcatcttc aacagtcatt atacctttca atataaatgg tagtttagta
     6481 gactttacta actcctttat ttgtttaaca tttttaggaa gtactggttt tccatgtaat
     6541 gataaagtta ctagaccaca ggcatctata tcaactccaa ctgcaaatgc accagcttct
     6601 tcagctaatc ttatcttttt tataatattt tcattatccc aaggtttaat aaatacaatt
     6661 ccagctccat tatacttttt gactacatct aaattttcca ttaaaaaagc atctactgcc
     6721 gtatcaccaa ccattgcata aataccacta ttagcacatc ctgcaactac tggttctatg
     6781 taatctcttt cattaatttt tccacccata tttaaagtag ttcctgttac tggagctgca
     6841 aatataggtg cactcatttt ctttccaaac atctctatag atgtatcagg atttgataca
     6901 tcatgtatta ctctcatatt tacttttact ttttctaaac tcttgctatt ttctataaaa
     6961 gaagaaccac ttcctttacc tcccatgcca ggcacttcac ctgcacaagc aaccccatta
     7021 caaaccttac aaactttaca acttccattt aaattttctc tagcactttt taataattca
     7081 ttatattcca tctttaatcc cccattaact atagtaattt atattatata cattatatca
     7141 tattatattt acagtatatt tttcaaaaaa ctttttctgt ggataggagt tatgccatat
     7201 ttttcaatag cttcatagtg ttctttagtt ccatatcctt tgtgagattt aaacccatat
     7261 tctgggtata ctctatcata ttggtacatt atactatccc tagtaacttt agcaagtata
     7321 cttgctgctg ctattgatat agattttgaa tctcctttca caattggatt ttgagatatg
     7381 tcaatacctg gtattgttgc tgcatcaact aataaataat ccggtgcttt ttttaaacaa
     7441 tttatagcct ttttcattgc catatatgta gcatttaata tattaaattc atctatttct
     7501 tcattgttta ctatacctat tccataatct agtgcttctt cttttattac ttcaaaaagt
     7561 tcatctcttt ttgcctcact cagtttctta gagtcattta caccttctat tttggtatct
     7621 tttttaaata ctacaacaga agctacaaca ggtccagcaa gtggtcctct tcccgcctca
     7681 tcaattcctc caatatataa ataaccttta tcatagcctt cgttctcaaa tatatttata
     7741 gtttccaatc tttcttcttc ttttctaata ttatccagct tttttgctag ttttatagct
     7801 agaccttgta cagattttct ttcatcaact cttaacagct ctatatattc catatatttt
     7861 tctacttcta aagtctctat tatttcttta atctccctca cacttttatc ctgcatacca
     7921 ttccctcatc tttcgttttt aaaaatttaa tttttaaata tattaaaatt ttttaattag
     7981 agtttaattg catccactat agccacattt taaacatata ctacatccac ctgtatgtgc
     8041 aattccttca cttccacact cagggcacac aaatttttca tcttcttcaa aatttttatt
     8101 tattatttct ttggcttttt catctttatt atctttttct tttttattat ttttataata
     8161 ttctttttta ctattttctt ttttatcttc tttattattt tcttcctttt tttcatttat
     8221 taaaattcca ctccttttac atccatctct aaatattgtt actcctttaa gaccagcctc
     8281 ccatgcctta atatatatat tgaaaacatc ttcaactgta gtttcaaaag gtatatttat
     8341 tgtagatgat atagaagcat caatatactt ttgccatact ttttgcatgt ttattctttc
     8401 ttcatatcct atattcatag cagtaacaaa aaaatctggt aaacattctt catctgtaat
     8461 acctttcttg tccatatact ctttaacaat tggagtatat actttataat acacatcctc
     8521 atcatgcaag ctttcagttt ttcttatata tgaaagatta aatataggtt ctattccacc
     8581 actgatacct atcattgtag atatggaacc tgtaggaggt atggttaaaa gttgtgaatt
     8641 tctaagccca tactttttaa ccatttcctt agtgtcttca tttagatttt ctataaaaaa
     8701 cttagattta gaaatatttt catatttata ctcactaaat gttccatatt ctttagcaat
     8761 taatgctgat tgcttcacag cttcatttaa catagtctta ccaattaaat cacatagttc
     8821 taaagattca tcagaaccat atcttattcc catttttata agcatatcag ctatacccat
     8881 tacacctaaa cctatctgtc tatacttatg tacactatct ctttgttctt gtaatggatg
     8941 caagttcaat ccctcatcta agacatcatt taaagctact acagcttctc taacgcaaga
     9001 ttttaattta ttaatatcaa aagtagcatg ttcaccaaat ggttgtatta taaactctga
     9061 caagtttatt gagccaagta gacaacttcc tccacttgga agtggttctt ctgcacaagg
     9121 atttactcct gaaaagcaaa agttcttatc ttcactcata aaattatatg tttttatttt
     9181 atcccaaaac aatatacctg gttctgcata atcccagtta tttttcacaa atttattaaa
     9241 taaatcataa gcatttacta tcttaactat tttttctcca gtagtttcaa cttcaaaagt
     9301 acaattatat aacttttttt ctcttacagc ttgcataaat tcatctgtta ctcttacaga
     9361 aatatttgct ttagtaacct tctccaaatc tgtttttata tctataaagt cctctatctc
     9421 tggatgattt acatccatag aaatcataag agctcctctt ctccctcttt gaccaattaa
     9481 gccagttgtc atactgtaaa gttccataaa agagactgct ccagtcgtat gttttgcagc
     9541 attgttaact ttcgctcctt ttggtcttag ctttgaaata tcaattccaa ctcctccacc
     9601 ataactaaaa gttctagcaa gtttttttgc agtatcaaat atatcttcta tgctatcttt
     9661 tggtggttct attacataac aattagaata tgttactttt tttccttctt tctgtaatcc
     9721 tctatttgct aaaattcttc cagcaaatag aaatcttttt tgtcttatta gttttgatat
     9781 tcttgaacta ttatttgaaa ttctctttat ccattcatca aaactctcac cattatgtct
     9841 atacttgttc ttccatattg atttttgtaa ttcatcaaac tcccaagctt tagtccttat
     9901 ttcagagcgt ctttctctat ataatatgta gtgttttgct atatcttttc tatttgattc
     9961 cataagtagt acttcaacta tatcttgtat ttcttctacg gataagcatt tttccataga
    10021 attatagtca ttagctattt ttcttgatat atcatatgcc aactctctat ctactccacc
    10081 ttcactattt atactagctt tatagacagc atcttcaatc ttacttttac taaaaagttc
    10141 ttttgtacca tctcttttta ttatgtactt catttttaaa cctccaataa attagtatta
    10201 aattaataca cacattaact ataactctaa acaaactttt tatatatttg tatattttca
    10261 tattaattat ctaacataat atatattata ctattttata ttcaatataa cactccttat
    10321 agttttctta acttgtaaaa tatcttccta aaaaactcaa aaataatata aaaaagtata
    10381 taaaagtcaa attatactct tatatacttt atttttttat atttttttca ttttaacttt
    10441 gattaa
//
LOCUS       DAGSRM010000118        10300 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.122, whole genome shotgun sequence.
ACCESSION   DAGSRM010000118 DAGSRM010000000
VERSION     DAGSRM010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10300
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.122"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            154..1578
                     /locus_tag="MJE33_003265"
     CDS             154..1578
                     /locus_tag="MJE33_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HBY9649181.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANQSEEDSTSLEEAKAKMSDMKSASKNSEKNIEEKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLNIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDTLFKRLESK"
     gene            1613..3742
                     /locus_tag="MJE33_003266"
     CDS             1613..3742
                     /locus_tag="MJE33_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBY9649182.1"
                     /translation="MKKKKITSRQKKIILMIVENSKKNIPITISEIAGTLELSSRTVL
                     RDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYSKEERNLI
                     ILSKLLVSNEPVKSYYFAKILKVSEGVLNNDFALASKWLERFDIELVRKPGLGVYLKG
                     QEKSFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLMDRCIIRK
                     VEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITEEFK
                     LAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITNKMI
                     DLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYAYDGVE
                     KISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSRFLS
                     TKIENKFPNLNILETISAINIDEEYLKEKDVDLIVSTVELNTSLNYICVGPFMSLDDE
                     QIIKEKIKSIAQNKLINLNAKNDTKSKNKVYEQITESMNIGKDILQFLEEIRFEKFES
                     KDLSELIEDSSKIFAKSSEDIISIKESLKERLKISIPYIEESKILLLHCMSERIDIMK
                     LSIIKLENRIVLDSNEEIDNVVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKINKF
                     SIEEMALEVKDIVFNFYTNRLKAFIDK"
     gene            3772..4194
                     /locus_tag="MJE33_003267"
     CDS             3772..4194
                     /locus_tag="MJE33_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBY9649183.1"
                     /translation="MSKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAN"
     gene            4213..5364
                     /locus_tag="MJE33_003268"
     CDS             4213..5364
                     /locus_tag="MJE33_003268"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBY9649184.1"
                     /translation="MKKAIQFGAGNIGRGFIGGLLVKSGYHVVFADVNEGILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKARKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEMYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEVHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSIGVKKAVAKIANISNDEELLNNIEKSYIFMKNL"
     regulatory      5470..5571
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            5754..6326
                     /locus_tag="MJE33_003269"
     CDS             5754..6326
                     /locus_tag="MJE33_003269"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBY9649185.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKGEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     gene            6390..7040
                     /gene="fsa"
                     /locus_tag="MJE33_003270"
     CDS             6390..7040
                     /gene="fsa"
                     /locus_tag="MJE33_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBY9649186.1"
                     /translation="MRFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHKGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGASLKL"
     gene            7192..10116
                     /locus_tag="MJE33_003271"
     CDS             7192..10116
                     /locus_tag="MJE33_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY9649187.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKI
                     GIKDKDPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTLG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGVGTALNLKEHIEKQIKEYDTLIEVKPVAFLNNNLEEELNDIEAEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPALNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVIKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LS"
BASE COUNT         4213 a          995 c         1952 g         3140 t
ORIGIN      
        1 attaaacaaa aactaaaatg acaaatgtca ttatctaaag tgacatagaa acggtttctt
       61 aaagttgtta ctataaaata taaactctat aataaaagta taacaaaagg gaatcatatc
      121 gatatagaat ataattcaag aggaggtaaa attatgagta gttctaatca atcgacaata
      181 aagacatcta tacagcgagt agggaaattt ttaagtggta tggtaatgcc aaatatagga
      241 gcttttatag catggggtct tataacagca ttatttatac caacaggatg gatgcctaat
      301 gaaaaattga gtactatagg tgacccaatg ataaaatacc ttttaccact attgatagct
      361 tatactggtg gtaaagcaat agcaggacaa agaggtggcg taataggtgc agcagcagct
      421 atgggagtaa ttgttggtgc agatatacca atgtttatcg gagcaatgat aatgggacca
      481 tttgcaggat gggtaattaa gaagtttgat aaatttgtag atggaaagat accaacagga
      541 tttgaaatgt tagtaaataa cttttcaata ggtataatag gtatgcttct tgcaatacta
      601 ggattttatg caattggacc agcaatagta gcaggaacag ctcttataga atctggagtt
      661 caatttatag ttagcaaaag tttattgcct ctagtttcag tatttataga acctggaaaa
      721 gtattattct taaacaatgc aataaatcat ggtatattag ggcctatagg tatagctgag
      781 gcaaaagaag caggtaaatc tataatgttc ttactagaaa gtaaccctgg accaggttta
      841 ggagttctat tagcatattg gatgtttagt agaggaagtg taaaacaatc agcaccagga
      901 gctgtaataa tacatttctt tggtggtatc catgaaattt acttcccata tattttaatg
      961 aatccagttt taatattagc tacaatggca ggtggagcag caggaatatt gactttttca
     1021 atattaggag ctgggcttgt agcagcacca tcaccaggaa gtatatttgc acttatggca
     1081 ctagctccaa agggaggatt acttccagta ttagcaggag ttgcagtggc aacagttgta
     1141 tcattcttag tggcagcacc atttgttaag agagcttcag caaatcaaag tgaagaagac
     1201 agtacttctt tagaagaagc taaagcaaaa atgagtgata tgaaatctgc aagtaagaac
     1261 tctgagaaaa atattgagga aaaacagctt gaagtaaatg agattaagaa gattgtattt
     1321 gcatgtgatg caggaatggg ttcgagtgca atgggagctt ccagatttaa aaatagaata
     1381 aaaaacttag acttaaatat agaaattaca aattcatctg ttgataatct tccagatgat
     1441 acacaaatag tagtaactca caatacatta gttgaaagag ttgctaagaa taattcaagt
     1501 gttgagatag tttcaataaa caatttttta aatgacccaa atttagatac attgtttaaa
     1561 agattagaat ctaaataaaa ataaaaagta ccactaatta ggagtggata atttgaaaaa
     1621 aaagaagatt acatctaggc aaaaaaagat aatactaatg atagtagaaa attcaaagaa
     1681 aaacattcca ataactattt ctgagattgc agggacttta gagttaagtt caagaactgt
     1741 acttagagat atgtctggaa ttgaaaaatg gtttgatgaa aatgatttta actttgtaaa
     1801 aaaaccagga gtgggattga ttttagagga aaatatagaa aatcaaaact ttataattga
     1861 gttgttagaa gaagaaaaaa tagaaaaaga atacagcaag gaagaaagaa atttaattat
     1921 attatcaaag ctactagttt ctaacgagcc agtaaaatcc tattattttg caaaaatatt
     1981 aaaggtatct gaaggggtac tgaataatga ttttgcctta gcaagtaaat ggttggaaag
     2041 atttgatatt gagcttgtta gaaaaccagg gcttggtgtc tatttaaaag gtcaagaaaa
     2101 aagctttaga gaggcatatg taaatcttat atacgattca ttcaatgaaa aagaaatact
     2161 agatatggtc agaaatataa gtgaaaatat acaaacagat aaagcaatag aaattttaag
     2221 tgaaaataga cttttaaatt taatggacag gtgcataata agaaaggttg aagctacatt
     2281 aacaaagaag ctttcagatt tagatgtaaa tttagctgat agtgcatata tagggcttgt
     2341 agttcatatt tcattagcct tacagaggat aaaaaatggt gaaaatataa ctatggataa
     2401 agagtttcta aaagaattat ctataacaga agagtttaaa ttagctaaag aaatcgtaaa
     2461 aggtatggaa acagatttta acatggatat accagtagat gaggttggat atataactat
     2521 gcatataaga ggtgcaaagc aaaggtcatc ttcaaaccat aaggctttaa acttagatga
     2581 tatagaaata atggaaatta caaataagat gatagatttg gcggaagatg aatttaaaat
     2641 aagtttaaaa aatgacgaga ggttatttaa agacctagca aatcacttag ggccttctat
     2701 aaatagattg aatatgggat tagaaataag aaatcctctt ttagatgaaa taaaaagcaa
     2761 gtattcatat gcctatgatg gagttgaaaa gatttctaga attataaaag ataaactaaa
     2821 tataaattca atacctgaat cagaaatagg atatattgca atgcactttg catcagccat
     2881 agaaaaaaat cttatgatga atactaatat aaatattgtt gtggcatgtc ccactggaat
     2941 aggtacttca agattcttat caactaagat tgaaaataaa tttcctaatt taaatatact
     3001 tgaaaccata tcagctataa acatagatga agagtattta aaggaaaaag atgttgattt
     3061 aatagtgtct acagttgagt taaatacaag tttaaattat atatgtgtag gtccatttat
     3121 gagtttagat gatgaacaga taatcaagga aaaaataaaa tctatagctc aaaataaatt
     3181 aatcaattta aatgcgaaaa atgatactaa gagtaagaat aaagtatatg aacagataac
     3241 agaaagtatg aatataggaa aagatattct tcaattttta gaggaaatta gatttgaaaa
     3301 atttgaaagt aaagatttaa gtgagcttat tgaagactca tcaaaaatat ttgcaaaatc
     3361 tagtgaagat ataatctcta taaaagaatc gttaaaagaa agattaaaaa tttcaattcc
     3421 atatatagaa gaatcaaaaa ttctattgtt acattgtatg agtgaaagaa tagatattat
     3481 gaagttatca ataataaaac tagagaatag aatcgtgcta gattcaaatg aagaaatcga
     3541 caatgtagtt tttatgttac ttcctaaaaa ttcaccaagt tatcaaagac aaataatgag
     3601 tgaaataagt ggttcattaa tagacaatat aatttttact aataagataa ataaattctc
     3661 tatagaagag atggcattag aggtaaaaga tatagttttt aatttttata caaacagatt
     3721 aaaagcattt atagataaat aaaattaata ttaaaaagga aggttgatac tatgagtaaa
     3781 gtattgaatg agaataatat atttttagga ttagatagtg tttcaaagga agaagctata
     3841 actttagcag gaagaaaatt agttgaaaat ggatatgtaa aagaagaata tataccagca
     3901 atgttagaaa gagaaaaagt aatgactaca tatatgggaa tgggagttgc tattccacat
     3961 ggtgtaaatg aagcaaaaaa agaaatatta tcttctggaa tagttatatt acaattccct
     4021 aatggtatag attttgatgg agaaaaagca tatttattaa taggaatagc tggtgttgga
     4081 gatgaacatt tagaaatact atctaatata gcagtagttt tagatgatga cttaacagaa
     4141 agactaaaaa attcaaatga caaacaagct tttatggaag cttttgccaa ctaatcttag
     4201 gagggattta aaatgaaaaa ggcaattcag tttggagcag gaaatattgg aagaggtttt
     4261 attggaggct tgttagttaa gtcgggttat catgtagtat ttgctgatgt aaatgagggg
     4321 atattaaact caataaacaa agataaaaag tatactatac acataagaga tgtagaatgt
     4381 atagatgaag taattgataa tataagtgcg gtttcatcta taaaagaaga aataatagat
     4441 gagattgtac aggctgaaat aataactaca gcagttggac ctttagtttt gactaaaata
     4501 gcctctacaa ttgcaaaagg tataaaggct agaaaagaaa agggattaac aagtaatcta
     4561 aatataatag catgtgaaaa tgcaatatac gcaagttctt ctttaaaaga agaggtactt
     4621 aagtatttaa ataaggaaga agtagaatat ttagagatgt atgttggttt tccaaactgt
     4681 tcagttgata gaatagttcc tcctggtaaa aacgaaaatc ctcttgatgt aacagttgaa
     4741 aatttttatg agtggaatgt tgaaaaacaa ggatttaaag gggaaatacc aactatagtt
     4801 ggaatgaatt tagctgataa tcttatggct tatatagaac gtaaattgtt tacactgaat
     4861 acaggtcatg ctataacagc ttatatagga tatttaaaag gatataagac tatagaagag
     4921 agtataaaag ataaatttat ctgtgatata gtcaaaagtg caatggtaga aagtggagaa
     4981 ggattgatta aaaaatacaa ttttgactca gaagtgcact ataaatatat tgataaaatt
     5041 ttaaatagat ttaaaaatcc atatcttaat gatgatgtat taagagtcgg aagggaacca
     5101 ttaagaaaac taagtgataa agatagattg attaaacctt taatgacagc aaaatcatat
     5161 ggattatcag ttgataattt gattcttgga ataggtgcag cgcttcatta taataatagt
     5221 gaagatactc aaagtgtaga attacaagag ttgataaagt ctattggggt aaaaaaagcc
     5281 gttgctaaaa tagctaatat aagcaatgac gaagaacttt taaataatat cgaaaaaagt
     5341 tatatattta tgaaaaattt ataaatttac ttgaaaaaaa gtagttctga taaagtaact
     5401 ataatatatt attctacaca gtttgaatta tttgttgaat aatagagaat tataatatat
     5461 aattaaaagg aataaattaa tatatttacc tgtatatgtt tgataatatg gttcaaatgt
     5521 ttctactaag ctaccattaa tagcttgact acaggagtga agaagatata gtttatactt
     5581 attttaaatt atatattaag ttaattactt tgaattttaa gctcctgtct atgatataga
     5641 caggagcttt tttagtattt taatataaaa tacttacaag aatagatagc aaaattagaa
     5701 caaatataat ttttttatta atataatttt agaaagggct aggtggaagt tcaatgaagg
     5761 ctttacaaga aaagatattg agagaaggta gtgtttcagg aaatgatata ctaaaagttg
     5821 atagtttttt aaaccatcaa attgatgtag cttttttaaa tgaaatagga agagaattta
     5881 aagagagatt taaaggagaa aaggtggata agatatttac aatagaggct tcaggaatag
     5941 ctatagcatc aattgtttca cagtattttg ataatgcacc tgtagtattt gcaaaaaaat
     6001 cagaatctaa aaatttggat aaagatgtat atgaaactaa tgtatattca tttactaagg
     6061 caagagagta ttcagttaaa gtttctaaaa agtacataaa caaaggcgaa aatatattga
     6121 tagtggatga ttttttggca aatggaagag ctgctcttgg acttaaagat ttaattgagc
     6181 aagcagaagc taacttagtt ggagtaggaa ttgtcattga aaaaggattt caagctggag
     6241 gagcactttt aaaggctaat gatgtaagat tagagtcttt ggcagtagtt gaatctatag
     6301 ataatggaac tgtaaaattt aggtaattta tgatattata tatttgtgtt tagcacatat
     6361 tttaactatg taattatgga ggatttttta tgagattttt tatagacaca gccaatattg
     6421 aagaaataaa agaagcaaat gatttaggtg taatctgtgg tgtaactact aatccgtctc
     6481 taatagctaa agaaggtaga gattttatag aggtagttaa agaaataagt gaaatagtag
     6541 atggaccaat aagtgcagaa gtaataagtt tagaacataa aggtatgata gaggaagcag
     6601 aaaaattatc taagatacat aaaaatatag ttataaagat acctatgaca gcagaaggtc
     6661 taaaagcggt gaaagtacta tcaagtaaag gtataaagac taatgtaacg cttatattct
     6721 ctgctggaca agctctttta gctgctagag ctggagctac atatgtaagt ccttttgttg
     6781 gtagattgga tgatatatca caaaatggtc ttgatttaat tgaagaaatc gttgatatat
     6841 ttagtgtaaa tagtattgag gctcaaataa tagttgctag tgttagaaat ccaatacatg
     6901 ttttacaagc tgctagaatg ggagcagata tagcaacagt tcctcttaaa gttataaacc
     6961 aaatgattaa gcatcctttg acagacaaag gaatagatag ctttatgaag gattgggaag
     7021 gtgcttcttt aaaattataa ttataaacta cgctttataa gtatattaaa gaggttatta
     7081 tggaaattct aagtaataag gctaggctat tttgcctagt cttattttgg tgtataatat
     7141 attgaaacat attattgaaa tagattaatt ttttaagtga ggaggcgaaa aatggataaa
     7201 atattaagag agatacaaag agaagataaa aaaaatccat atactgacca agaacttgct
     7261 gaaatgttaa atgtagctag gtctgaagtg atatcagtta ggaaaaaaaa taatatttta
     7321 gattcgagag aaagaagaaa aaaaatatta ataaaagata tatcaaaaat tgtaagtgaa
     7381 aatcctcaaa tgtctgaaag aaaaataact gaaatattaa tagaaaatgg atataatata
     7441 tcgagaagtg cagtttcaaa attattaaaa gaggaaaatt taaatcagtc aaagattggg
     7501 attaaggata aagacccagc atcatcagga aagtatgtga aggtgagtaa agttgtagaa
     7561 gaagatggat ttgaagaatt gataggtata aagggaagtt tgaaagaaaa ggtaaattta
     7621 gctaagtcag caataatgta tcctcctaat ggccttcata ctataatata tggagaaaca
     7681 ggagtaggta aaagtgaatt agctacatgt atgtataaat atgctataaa aaacaacatt
     7741 aaagaagaga acagcccatt tatagtgttt aactgtgcag actatgcaga aaatcctaat
     7801 ctattgatag ctcaactctt tggggtagta aaaggtgcat atacaggagc agatgcaaat
     7861 agagaaggtc ttgtagaaca agctaataat ggaatattgt ttttagatga aattcataga
     7921 ttgcctgctg ctgggcaaga aatcttattt tcattaatag atagaggaga atttagaagg
     7981 ttaggagaaa gtagttctaa tagaaaggca aatgttctca taataagtgc tacaacagaa
     8041 aatccagatt ctaatttact tcaaacattt agaagacgta taccaatggt aataaattta
     8101 cctaatatga gtgaaagacc aaaatctgaa agatatgaaa taataataaa gttttttgaa
     8161 agagaagcaa agcgagtaaa caagaatttt ataataacta aagaagttat gtgtgcactt
     8221 atgaattata agtgtactgg aaatataggt cagttaaaga gtgatataca agtaacttgt
     8281 gctagagctt ttagtaaatc gatattttct agtgataaag taattataga tttagatagt
     8341 ttaagggatt atataaaaag tggatattat gattgtaatt gtaaaaatga aaagtattct
     8401 gagttttcag ttgaggatat atatatagat attagtgaat tggtgagtgg aaaaaataga
     8461 attttatctg ataatgagat aagcgagata tataattatg cagaaaaaga gcttaaggtt
     8521 ttggaaagta agtgcttttc agaagaagag ttaaaaaatg catttataga aaagttagat
     8581 aagaagttta atgctattaa aaacaataaa aatttatctg atagaagaag tcgaatagac
     8641 tggggaggac aattaaaaga gtctacactg gagataatgg ataaggtaat tgtatttatt
     8701 aaggagcagt ttaagcatgt aaatcaaggg ctgtacttgg ctctagcaat tcatattgag
     8761 catgctatag gtaggataaa agatggaaaa actataataa atccgagttt agataaaata
     8821 aaagcatcaa tgccaatgga atatgagttg tcaagatata taatgggtat agtagaagat
     8881 ttgacacaag taacatttcc agaagatgag cttggatatt tggcttatta tataaataaa
     8941 ttttgttata atgaggaaag tataaaagat aaagttaaag tagtaatagt tacgcatggt
     9001 aaagttggaa ttgaaatgtc taaagttgta aatcatattt taggtataga atgtacacta
     9061 ggtatagaaa tagcacttac agattcacca tcagagggga tagagcatgt attagaagag
     9121 cttcaaaaga tagaagctag aaaaggtatt ctagttctaa ttgatatggg ttctttggta
     9181 attcttggtg atgaggttga aaaaaggctt ggaattagat gtaaaactgt aaatagagta
     9241 gatacattat tggcaatgga agcaggaaaa cttgcaacca ttgaagggaa aagtttagat
     9301 ggtataatag ctgatttaaa gaaaaataag aattatgcta tgataaatac aaataaattt
     9361 agttacagaa aaaacgagta tggaaagaaa aatgtaatca ttactttatg tttgagtggt
     9421 gtaggtacag ctttaaattt aaaagagcat atagaaaaac aaattaagga atatgacact
     9481 ttaattgaag taaagccagt tgctttttta aataataatt tagaagaaga gttaaatgat
     9541 attgaagctg aatataatat tattgctatc tgtggaacta ttgatataga ttataaaaac
     9601 gtgcctttta tttcttataa tgaggtactt ggtaaaaatg gtattaataa agtattagag
     9661 catttaaata gtaaacaacc agctttaaat attgaaaacc agttgaataa tttaatacat
     9721 gaagatttga ttttatacaa ttttgaagga atttcaaagg agtatataat tgatactctt
     9781 gtttcaaaac ttgaagaagg tggatatgtc gactcaaagt atatattaag tgtatataaa
     9841 agagaagcaa tgggaagtgt tgtaatggcc tctaaggtag ccgtgccaca tggacttcca
     9901 gaaaacgtta taaagcctgc aatagcaatt gcgaggttga ataaacctat agtatgggac
     9961 aataaattta tggtggattt ggtggtacta ctggcactta aagaaaataa taagaaagaa
    10021 atacgaagct tattttcaaa gattaatgac cagcatactt tagaagtttt attaaatact
    10081 gaagataaaa atgaaataaa agaactttta agctaattgt tagtaccgag aagaaagaca
    10141 aaaaaatttg ctttagtagt ataaaattta aaactactca attgctaagt agttaattac
    10201 tctatataag gctagttcta tgaaatttat ttatatgatt ttatagaatt agcttttttc
    10261 ttggtaaata aaatatatga atttatttat gtaaaaagta
//
LOCUS       DAGSRM010000119        10263 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.123, whole genome shotgun sequence.
ACCESSION   DAGSRM010000119 DAGSRM010000000
VERSION     DAGSRM010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10263
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.123"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            241..1164
                     /locus_tag="MJE33_003272"
     CDS             241..1164
                     /locus_tag="MJE33_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HBY9649188.1"
                     /translation="MGSGKVGTSLANYFLSKGYCVSGFYGRNQLSLLESTNLTKTKIY
                     SNLKDIIYENDILFITTSDDSIEIIDKKLSKFNLKHKYICHTSGSLKSSILFCSKKAG
                     ALIYSIHPIFAFSNKNTNIKKMKDICFSIEGDNEQDDLVIQNFIDDLGNQFFIRDKDT
                     SSTYHLANVLVSNLVLSLLDIGVSYFIKLGLSEEESLKAISPLVKKNIENILDNGFTK
                     ALTGPVSRGDITPIRKHLSVLDKKDEDIYKILSLNLLKIIALGNDNSLKGIKNITIEN
                     AIENLISKSEKYKEIYKLLGGKNDEKYHTDF"
     gene            1139..1966
                     /gene="panB"
                     /locus_tag="MJE33_003273"
     CDS             1139..1966
                     /gene="panB"
                     /locus_tag="MJE33_003273"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBY9649189.1"
                     /translation="MKNTIQTFKNAKYEGKKLSMLTAYDYSIAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGVKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LIKEGGAHAVKLEGGSNVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGNTSET
                     ARQLIEDAKLIEKAGAFSIVLEGVPTKIAEMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGMFGDFVPKFVKQYANIGDIMKDSIKNYILEVNTGAFPQEKHSFSINESELEKLY"
     gene            1985..2833
                     /locus_tag="MJE33_003274"
     CDS             1985..2833
                     /locus_tag="MJE33_003274"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBY9649190.1"
                     /translation="MLVKEIKLLRNIIKDWRKHGYSIGLVTTMGFLHEGHQSLIKKAI
                     KENDKVVVSVFVNPTQFGPNEDFNSYPRDIDKDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPVHFGGVCLVVSKFLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNIDTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNKSLSLAKEELVKGNLN
                     PENIKELIIAKINSEHLAKIDYVEIVDSETLQPVKQIEHSILVAIAVFIGKTRLIDNF
                     TFELNI"
     gene            3244..3426
                     /locus_tag="MJE33_003275"
     CDS             3244..3426
                     /locus_tag="MJE33_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649191.1"
                     /translation="MFKDKIDECVHIMTAYIASLKEYYSFIETQIGDFIKKYGEDVVE
                     LCLHRVMILLCECGLA"
     gene            4258..5172
                     /locus_tag="MJE33_003276"
     CDS             4258..5172
                     /locus_tag="MJE33_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HBY9649192.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTNKNYNLAMEA
                     DHYKKQFEDLLSYTVSASNGIIRPDILYSEELVTTLTSVAEQKTEEFTGIEINKNITT
                     RELNLQSGVNPQVGQDLVNYVAQLNSDAIRLLDGLINFKERVLDGVLSCTIFTSNYPL
                     LLEHIIHEANLYRSYVVDLENKIDIESKNAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFAIKFNELIEKTNEMTDSNIKNITEETLNETVEFKDFKEAGASGIEQCKIK
                     SIILPLLADHVLREANHYIRILESYKNM"
     gene            5562..6209
                     /locus_tag="MJE33_003277"
     CDS             5562..6209
                     /locus_tag="MJE33_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBY9649193.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTALKKRYFDAYYCKTRNEALQQILD
                     LIPQNHVVSWGGSETLKEMGVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESICAYMVTTRISRPAKKIKVILVGENLGL"
     gene            complement(6376..6945)
                     /locus_tag="MJE33_003278"
     CDS             complement(6376..6945)
                     /locus_tag="MJE33_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBY9649194.1"
                     /translation="MKKFIICIIMFAISVLVLAGCTNDNKIKNEVDSMFNSIKTQDAD
                     TVDKYFPKSGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKINNTDMVSIL
                     KKNPPKLGEMMPNPTMKDIDSAKKKEFNITLELVKSGDNYVCKVNQESAMKLLNVITG
                     GGIDYLADYNKDYYKQLDEEQKNYENKNS"
     gene            complement(6950..7849)
                     /locus_tag="MJE33_003279"
     CDS             complement(6950..7849)
                     /locus_tag="MJE33_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBY9649195.1"
                     /translation="MKLLSKVNNNIRLITQVAFTALTNGYVNGFLEGTIYGGESKKLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLHKVPFPVKIKKVYGDKYLKYLKYIVLIMFVIILPVAIVNVAGGGNPWFCKW
                     ICPSGTLLGGIPLILGNENLRESIGFLFSWKLSILLLILLMSIITYRPFCKYICPLGA
                     IYGFFNPISIYRFKIDKDKCTNCTACQTKCKLDIPIYKDVNSPDCIRCGECIKVCPQN
                     AITTTFSKDKKENNICAKKYRGI"
     gene            complement(7860..8009)
                     /locus_tag="MJE33_003280"
     CDS             complement(7860..8009)
                     /locus_tag="MJE33_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649196.1"
                     /translation="MSNFIKDNKKSISIIAISIIFIALGTYRGETNTVLTKAIMICLE
                     CIGIG"
     gene            complement(8002..8586)
                     /locus_tag="MJE33_003281"
     CDS             complement(8002..8586)
                     /locus_tag="MJE33_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBY9649197.1"
                     /translation="MKRFFIVVLGCICVLGMISGCSKKESKPVEKNKNNSEWFSNLDT
                     EDVDGNKVTKEIFSNKELTLINVWTTWCGPCVGEMPELEALSKEYESNNSNVAIKGLV
                     VEVDKTDMRTGLSNEEKNLVKDIMKKSDATYQQLTVSEELKKTDFKRVMEFPTTYFVD
                     KKGNFVGEKVVGANSKEDWKKIIDERLKMVKANE"
     gene            8975..>10263
                     /gene="cdeA"
                     /locus_tag="MJE33_003282"
     CDS             8975..>10263
                     /gene="cdeA"
                     /locus_tag="MJE33_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HBY9649198.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVG
                     SDALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQDEANSTFSFIVLFSLIV
                     GILFTVISYFFIKEISILLGATDKLLPYCITYGKVMILCTPFYILKFIFEYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGIIYFLSNKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGITTFLFNVVALKLAGENGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFVSSLLVFIIALVFA
                     PFIVRVFVSPDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSR
                     AFVFIIIGIIILPPMLNMTGLWLTVPFAEVITIFISIL"
BASE COUNT         3801 a         1392 c         1348 g         3722 t
ORIGIN      
        1 aacttatatt ttataaatat cataattttt ttactgatat tttattatat aaattttata
       61 gaattaattg tcaatttttt atgaaaatgg taagatattt atggatggta tcttgttata
      121 agaactatac tttaaatgtt tttaattatt ttgtcttatg taactcatat cctcaggaat
      181 gagattagaa attaagatgt tgtatcacaa agtggaggtg atactattaa aataggcttt
      241 attggttctg gaaaagttgg aacatctttg gcaaattatt ttttatcaaa aggttattgt
      301 gtttcaggct tttatggaag gaatcaatta tctttattag aatcaacaaa tttaacaaaa
      361 acaaagattt acagcaattt gaaagatata atttatgaaa acgatatatt atttattaca
      421 acatcagatg attctattga aattatagac aaaaaacttt ccaaatttaa tttaaaacat
      481 aaatacattt gccacacaag tggctcttta aaatctagta tattgttctg ttcaaaaaaa
      541 gcaggtgcat taatttattc tatccatcct atatttgcat tttccaataa aaataccaac
      601 ataaaaaaaa tgaaagacat ttgtttttcc atagaaggtg ataatgaaca agatgattta
      661 gttattcaaa attttataga tgatttggga aaccaatttt ttataagaga taaggatact
      721 tcatccactt atcatttagc taatgtttta gtttcaaatt tagtattatc tttactagat
      781 ataggagtaa gttactttat taaattaggt ttatctgaag aggaatcttt aaaagctatc
      841 agtcctttag taaaaaaaaa tatagaaaat atacttgata atggctttac taaagctctt
      901 actggtcctg tttctcgtgg agatataaca ccaataagaa aacatctatc tgtattggat
      961 aaaaaagatg aggatattta caaaatacta tccttaaacc tactaaaaat aattgcctta
     1021 ggaaatgaca attcattaaa aggtataaaa aatattacta tagaaaatgc aatcgaaaat
     1081 ttaataagta aatctgaaaa atacaaagaa atttacaaac ttttaggagg aaaaaacgat
     1141 gaaaaatacc atacagactt ttaagaatgc taaatatgaa ggaaaaaaac tatctatgct
     1201 tactgcttat gactactcaa ttgcaaaaat aatggatgag tgtgatataa atggtatttt
     1261 aataggagat tcattaggaa tggtcataaa aggtgaagaa aacactctat ctgttaccat
     1321 agacgaaatt atatatcata caaaagctgt aaaaaatggt gtaaaaaatg ccttaatcgt
     1381 aagtgatatg ccttttctat cttaccatgt ttctatagaa gatgcagtta aaaacgctgg
     1441 tagacttata aaagaaggag gagctcatgc tgtaaagtta gaaggtggct caaacgttat
     1501 aaagcaaata gaaagcattg taaatgctca aatacctgta atggggcatt taggattaac
     1561 tcctcaatca gtaaattcat ttggtgggtt taaggttcaa ggaaatacca gtgaaacagc
     1621 aagacaactt attgaagatg ctaaattaat agaaaaagca ggtgcattct ctattgtcct
     1681 tgaaggagtg cctacgaaaa tagctgagat ggtaactaat tctatttcaa ttcctacaat
     1741 aggtataggg gcaggaatta actgtgatgg acaaatttta gtttatcaag atatgttggg
     1801 aatgtttgga gattttgttc ctaagtttgt aaagcagtat gcaaatatag gagatataat
     1861 gaaagattca attaaaaatt acatcctaga agttaataca ggtgcatttc cacaagaaaa
     1921 acacagcttt tcaataaatg aatctgagtt agaaaaatta tactaatatt aaggatggta
     1981 caaaatgctt gtaaaagaaa ttaaattact aagaaatata atcaaagatt ggagaaaaca
     2041 tggctattct ataggccttg ttacaacaat gggattttta catgaaggac accaaagtct
     2101 tataaaaaaa gctataaaag aaaatgataa agtagtagtg agtgtatttg taaatccaac
     2161 tcaatttgga ccaaatgaag attttaatag ctatccaaga gatatagata aagattttaa
     2221 atattgtatg gatagtggag caacagtagt ctttaaccca tcaccagaag aaatgtattt
     2281 aaaaggtaat tgtactacca ttaatgtttc tggacttaca gattttcttt gtggagctaa
     2341 aagacctgtt cattttggag gtgtctgcct agttgtttca aagtttctaa atatagtaac
     2401 acctgataaa gcttatttcg gagagaagga tgcccaacaa ttagctgtaa ttaaaagaat
     2461 ggtcaaggat ttaaacatag atacagaaat aataggctgt cctataattc gtgaaaatga
     2521 tggattagca aaaagttcga gaaatacata tctctctgag gaagaacgaa aatctgctct
     2581 aatattaaat aagagcttaa gtttagcaaa agaagaatta gtaaaaggca atctaaatcc
     2641 agagaatata aaagaattaa taatagctaa aataaattct gaacacttag caaaaataga
     2701 ctatgttgaa attgtagata gtgagacatt acaacctgtt aagcaaatag aacattctat
     2761 acttgtggca attgctgtgt ttataggaaa aaccagacta attgataatt ttacatttga
     2821 gttaaatata tagtctataa ttttatataa tatatctaaa aaataataga atacctaaaa
     2881 agtaataaat tacctaaaaa ataataaatt gcttagaaaa taatagatta caataactaa
     2941 aattttaaaa ttaaattcct taaaagtatg attaattata attatataag tatattctta
     3001 ttacacttat attaatttat attaaatatc tactaactca aatattcata attaaacttt
     3061 aaactggata aattttcttt taagaatgaa agtttatcca gttttgcgat tataataacc
     3121 agttttacat atccacttgt aaaatatatc taagcaatat atcatactat atagatttat
     3181 gagtgaaata atatctgcta tttactgaat atttttattt ttcgtttttt agggggatta
     3241 gttgtgttta aagataagat tgatgaatgt gttcatataa tgactgcata tattgctagt
     3301 ttaaaagaat actattcatt catagaaact caaattggtg attttattaa aaaatatgga
     3361 gaagatgttg tagagttatg tctccataga gtgatgattc tactatgcga atgtggtttg
     3421 gcataaaatt aaatttatta aaatgggtaa gaagaatcct atataatatt cttcttaccc
     3481 attttaatat atcaatttca tagcttatcg tttaaaatat tttacatcca ttactagaca
     3541 actttacatt ctctttttaa ttctagtata tttaaatcat tttagttgct ccttatttat
     3601 tcttattact tgttttattt tataaataaa ataattcttt ttaataatgt tttttgttga
     3661 tattcctatc acacatccaa ttatttatca ttgtcttgaa attgtttgca tacttaattt
     3721 aatccaattg aaacactcca gcatagaatc ccctgtaaaa tagaagttgg attaaatcct
     3781 aacatctata cagaaaatac tactccaagc acaagcaata taatggaata tatttatcac
     3841 ttaatttttt atacttttta aatcccatac ctaaaacatt aagtgcagct attagaatta
     3901 ataattgttg tggtataaaa ttatgcttgc ttcatattta ttcatttttt cctataaaag
     3961 ttcttgtttt acaaaaaaca tttgttttgt atcaaaacaa tacttataaa ttacataatt
     4021 ctcataaaaa tttctaatat tacttgtact cttaataaat aatagccaac aagcacctta
     4081 aatcattata tctatatcta ataagaaaca cagtaagata attttttcgc ccataactta
     4141 ataaaaactg ttgatattag tagcaattga atttagttaa tttatttata atatacataa
     4201 atttaaatat tacgaataaa gtattataaa taggatactt tctaaaggga ggaaattatg
     4261 atagataatc aaaaatatgt tattttatca ctagaattac atttattttt ttcaagaatt
     4321 atgaaagagc atgctctttt tttagaagca ggattcacaa ataaaaatta taatcttgct
     4381 atggaagctg accattataa aaagcaattt gaagatttat tatcatacac tgttagtgct
     4441 agtaatggta taattagacc tgatatatta tattcagaag agcttgtaac tactctcaca
     4501 tcagttgcag aacaaaaaac agaagagttt acagggatag aaataaacaa aaacatcact
     4561 acaagagaat taaatctaca aagtggtgta aacccacaag ttggtcaaga tttagtgaac
     4621 tatgtagctc aacttaactc tgatgcaata agattacttg atgggcttat taattttaaa
     4681 gaaagagtct tagatggtgt actatcatgt actatattta catcaaacta ccctctactt
     4741 cttgaacata taatacatga agcaaattta tatcgttctt atgtagttga ccttgaaaat
     4801 aaaatagata ttgagtcaaa aaacgctaaa gaaatagaat tattctggga ccatattatg
     4861 atggaacatg ctctgtttat gagaggatta ctagacccct cagaaggtga actaataaat
     4921 acttcaaatg attttgctat aaaatttaat gaattaattg aaaaaacaaa cgaaatgact
     4981 gattctaata tcaagaacat tacagaagaa actctaaatg aaactgttga gtttaaagat
     5041 tttaaagaag caggagcatc aggaatagaa cagtgtaaga taaaatctat aatattacca
     5101 cttttagcag accatgtttt aagagaggca aatcattata ttagaatatt ggagagttat
     5161 aaaaacatgt aattaaaaat tacttttaaa ataatctttg catatttaga taaaataaag
     5221 gtacagttaa ctgtaccttt attttttata aacttataaa atttaaacaa gttattcata
     5281 aaataaaata ataatcatct ataacccaca actttatttt atattaaata aaaagtatta
     5341 gtgattatgt tttataggta tttctttatt cagttattta ttaaaaacta atagtaaatc
     5401 ttatactaat cgcatattta tgttataact atattgtaac tattttcgta atcttaggaa
     5461 aaggttactt ataaaattaa ccttatacaa taatatatcg tataatctta attagcataa
     5521 tagttacaat attaaaattt aaatatggga gtgattttag tatgataaga caaacaccat
     5581 tagagaaacg atatgacaag ctaggtcctc atattgtaac agcactcaaa aaacgttatt
     5641 ttgatgctta ttactgtaaa actagaaatg aagctttgca acaaatttta gatttaattc
     5701 ctcaaaatca tgtagtatca tggggaggat ctgaaacact taaagaaatg ggcgtacaaa
     5761 ctgctgttag agagaaagga tttaaagtta ttgatagaga tttagcaaaa actccagaag
     5821 aaagaactga aattatgcgt caagcacttc tatgcgatac ttttttgatg agtagcaatg
     5881 caattagtga agatggacaa ctctttaata ttgatggaaa tggcaatcgt gtagctgcaa
     5941 tgatatttgg tccaaaaaat gttatagttg tagctggtat gaataaaatt gtaaaaaaca
     6001 ttgatgatgc aatacaaaga actagaacaa ttgcatctcc agcagttgtt cagcgttttc
     6061 aagatgtcaa tacaccatgc tatattaatg gaagttgtat agactgcaca agtcctgaaa
     6121 gcatttgtgc atacatggtt actacaagaa tcagtcgtcc agcaaaaaaa ataaaggtaa
     6181 tattggtagg agaaaatctt ggtctataaa atatacatac atgtaaatag ctctataaat
     6241 aataaaatca tatgaaataa cttatttcta aaagtggtag aataaaagct tctacaagac
     6301 tttcattcta ccacttttat attcttttaa tttgtgtttc tatattatta attgctccga
     6361 ttttcatata tattactaac tatttttatt ttcataattt ttttgttctt catcaagctg
     6421 tttataataa tctttattat agtcagctag ataatctata ccacctcctg taatcacatt
     6481 taataatttc attgcagatt cttgattaac tttacaaaca taattatcac cagatttaac
     6541 aagctctaat gtaatattaa attctttttt ctttgcagaa tcaatatctt tcatagttgg
     6601 atttggcatc atttctccta gtttaggagg attctttttt aaaatagaaa ccatatcagt
     6661 attgttaatt tttaaatcta cagtcacttt atccttatta ttctttatat ctgaaatttc
     6721 ccaagtcata tttttagaat acattttaaa tccttcttta tcttcatata taagattacc
     6781 taaccctgat tttgggaaat acttatcaac agtatctgca tcttgtgttt ttattgaatt
     6841 aaacatacta tccacttcat tttttatctt attatcatta gtacaaccag ctaacactag
     6901 tactgatatt gcaaacatta taatacatat aataaatttc ttcattaaat tatatccccc
     6961 tatacttttt tgcacaaata ttattctctt ttttatcttt tgaaaaagtt gtagtaattg
     7021 cattttgtgg acaaactttt atacattcac cacacctaat acagtctgga ctatttacat
     7081 ctttatatat tggtatatca agcttacatt ttgtttgaca tgctgtacaa tttgtacatt
     7141 tatctttatc aattttaaat ctatatattg aaataggatt aaaaaaacca tagattgctc
     7201 ctaaagggca tatatacttg caaaatggcc tataagtaat gattgacatc aataatatca
     7261 atagcaaaat acttaacttc cagctaaata aaaatcctat tgattctctt aaattctcat
     7321 ttcctaaaat aagtggtatt cctcccaaaa gtgttcctga agggcaaatc catttacaaa
     7381 accaaggatt tccaccccct gctacattta caatagcaac tggcaaaata attacaaaca
     7441 ttattaagac tatatacttt agatatttta gatatttatc accataaact ttttttatct
     7501 ttactggaaa gggtacttta tgtagtaaat cttgtacaag tccaaaggga caaagccaac
     7561 cacaaacaaa tctgccaaaa aaagcaccaa atgccattaa aaaaccaaca acataaaatg
     7621 agaatttata ctctctgctt gataaaactg cttgtaatga acctattggg catgctccaa
     7681 gtgcacctgg acatgaataa caattcaatc caggtacaca taatttttta ctttctcctc
     7741 catatatagt tccttctaaa aatccattta catatccatt tgttaatgct gtaaatgcaa
     7801 cttgagtaat aagtcttata ttattattta ctttagataa taacttcatt cctcttctcc
     7861 taaccaatac caatacactc aagacaaatc ataattgctt tagttaatac tgtatttgtt
     7921 tctccacgat atgtacctaa tgcaataaat ataatactaa ttgcaataat agaaatgctt
     7981 tttttattat ctttaataaa attactcatt ggcttttacc atttttagtc tttcatctat
     8041 aatttttttc caatcttcct tactatttgc acctacaacc ttctcaccca caaagtttcc
     8101 tttcttgtct acaaaataag ttgttggaaa ctccataact cttttaaaat cagttttttt
     8161 aagttcttca gatacagtca attgttggta agttgcatct gactttttca taatgtcttt
     8221 aactaaattt ttctcttcat ttgaaagacc agttctcata tcagttttat caacttctac
     8281 aactaatccc tttatagcta cattggaatt attactctca tattcttttg ataatgcttc
     8341 aagctctggc atttcaccca cacatggtcc acaccaagta gtccagacat taataagagt
     8401 aagttcttta tttgaaaata tctcttttgt taccttattt ccatcaacat cctccgtatc
     8461 taaattacta aaccattcac tattattctt gtttttttct actggcttag attctttttt
     8521 gctacatcca gaaatcatac ctaaaacaca aatacatcct aaaactacta tgaaaaacct
     8581 tttcatatct cacactccta gaatttatat agtatataaa atcgctgctt gaaataaaaa
     8641 taacttatct tctatattaa agtaattata tttaataaat aagagctaat ttaaaaaaag
     8701 atatacagtt aaaatctatt tcttttcaga caaaatctta tatatatata atagcttgta
     8761 aatcttaaga ttatatacat atatttctaa agattttctt aagaaacagt tagattttac
     8821 taatataagt ttattaatta gcactatact aatttatctt aatatgattt tttgttgatt
     8881 ttaagctgtt tatcgtataa ataaaatttt tcttgactat atagttacta tatagcttat
     8941 tatatataga gagtaataaa aggaggtaaa atttatggaa aatttattta caagaaaatt
     9001 cactactttt gaatttctaa aatttgtttc tcctgcaatt atatccatga tatttatatc
     9061 tttgtataca ataatagatg gcatctttgt atcgacatta gttggttctg atgctcttgc
     9121 tagtataaat attgtactac ctataattaa ccttgtttgt ggatttggaa taatgatggc
     9181 aactggtgga ggtgctatcg tttctatacg tatgggtgaa aatagacagg atgaagccaa
     9241 ctctacattt tcttttatag ttttgttttc attgattgtt gggattttat tcacagtaat
     9301 ctcatatttc tttatcaaag aaatatctat tttgcttggt gcaacagata agttattacc
     9361 atattgtata acttatggta aagttatgat tttatgtaca ccattttata ttttaaaatt
     9421 tatatttgag tactttgcaa gaactgatgg aaattctaaa tttagtttat ttctatcagt
     9481 cattggtggt gtaacaaata taatattgga ttatgtattt attaaatatt ttggaatggg
     9541 tcttttagga gctgcagttg caactgctat aggtattatt ttaacttgtg ttttaggtat
     9601 tatttacttc ttatctaata aatctacact aaaattaaga aaaccaaaaa ccgattttag
     9661 acttataaga gatactatga taaacggttc ttctgaaatg gttacagaat tatctacagg
     9721 aattacaaca ttcttattta atgtagtagc tttaaaatta gcaggagaaa atggacttgc
     9781 tgctcttact atagtattgt atgctcattt tttaatgaca tcagtctatc taggattcgc
     9841 tgctggagtg tctccattaa taagctataa ttttggtgct gaaaacagtg ataaattaaa
     9901 agaaacattt aaacattctc taaaatttat atttgtttct tctcttttag tgtttattat
     9961 tgctttagta tttgcaccat ttattgttag ggtctttgta agtccagata acacagtatt
    10021 taaactagcc ttacaaggat taaaaatatt tgcatttgct tttttgtttg ttggtataaa
    10081 tatatttgca tcaggatttt ttacagcatt tcacaatgga aaaatttcag ctattatatc
    10141 ttttagtcgt gcctttgttt ttataatcat aggaatcata attcttcctc ctatgttaaa
    10201 catgactgga ttatggctta cagttccatt tgctgaggtt ataaccatat ttatatctat
    10261 tct
//
LOCUS       DAGSRM010000120         9910 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.124, whole genome shotgun sequence.
ACCESSION   DAGSRM010000120 DAGSRM010000000
VERSION     DAGSRM010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9910
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.124"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            145..1389
                     /locus_tag="MJE33_003283"
     CDS             145..1389
                     /locus_tag="MJE33_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBY9649199.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGLHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLTNKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALVTSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
     gene            1712..2809
                     /locus_tag="MJE33_003284"
     CDS             1712..2809
                     /locus_tag="MJE33_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-diphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBY9649200.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEIIIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDTLLHEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     VFKGIDESKIKVNGIPVKKEFKRIEHVNHSTKKNILIMGGGLGLLPKSEQFYKELNSL
                     EGVKTTVITGNNKKMYYKLYGRYENIEVVGYTNEVYKYMKDSDLIISKPGGITLFETI
                     YSELPILAFNPFLQQEIDNASFILNNEIGRILGKNKKYYVDEIKDLIYDDATLKEMSS
                     NMKELKKQFDNNTLENILFSLDEQGACRECM"
     gene            2800..3498
                     /locus_tag="MJE33_003285"
     CDS             2800..3498
                     /locus_tag="MJE33_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBY9649201.1"
                     /translation="MYVVGLIIVVALIFLVHSIIPTYYNKLLNKKVLKNMAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENDIQAMFFVVAKNAEKEPEIIKRMLRENHIVGLHSLEH
                     RNAWLYSYSYVKKDFIESTNIMKNLGVDVNYYRPPWGHTNIFSNSFVKKYNLKMTLWD
                     VMAEDWEKDSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKIAIPK
                     LKASGLKFVTPERM"
     gene            3501..4565
                     /locus_tag="MJE33_003286"
     CDS             3501..4565
                     /locus_tag="MJE33_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBY9649202.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFVMMFIYVACEGINIYLGMKALNQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGIMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVIGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVESSRKVIISQI
                     EEYVKGAQYIKQNPKLVVQILVITIVQITAMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     VVHVKSSEKVKAVKKVAYVR"
     gene            4748..5452
                     /locus_tag="MJE33_003287"
     CDS             4748..5452
                     /locus_tag="MJE33_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY9649203.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LVDSTIDLIILDVVMPVKTGFVACNEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEREPNILKTKELCVNLDTQDVTLNGEPVF
                     LTTIEYSILILMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            5445..6767
                     /locus_tag="MJE33_003288"
     CDS             5445..6767
                     /locus_tag="MJE33_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY9649204.1"
                     /translation="MINKIKPSKLGVILLIYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNQLMDNFQTYVNNNGIRADDGDDIAKWNSDNWFVSMKIYKGNKVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIVVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSH
                     TLENQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPIVFLIKVEDSKLLIIQKNRV
                     SNFSKNVESFGIGIKNCEKIVAYHNGSINVNSDDKEFAIEISIPAKES"
     gene            6954..8327
                     /locus_tag="MJE33_003289"
     CDS             6954..8327
                     /locus_tag="MJE33_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649205.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISMAFYTLKRDINKGMFREE
                     LKGFESEEVEEFNGKSKIATILVALGFLADIIAMYIFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKFAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISAAAICGVSPIELAKRNFVPVITGLVVTT
                     IVAMFII"
     gene            complement(8699..9637)
                     /gene="pdaA"
                     /locus_tag="MJE33_003290"
     CDS             complement(8699..9637)
                     /gene="pdaA"
                     /locus_tag="MJE33_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBY9649206.1"
                     /translation="MFRKVLHYLTLLSISIFFIVGCSNSQNNQNENQNKETQLQEDKE
                     KIDSGKDTSNVIVSDGTDKPSKATTNNDDNKLDVSSLDNTTLDWFYIPNNKHKTPEVN
                     TDIEFKFSDYDALYNGPTKDGQKTLYLTFDEGYENGYTTKILDTLKQNQVKAVFFVTT
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLKNFNDELYDVEKLYKDVTGKD
                     MVKFFRPPMGKYSEKSLAMTKNLGYKTVFWSFAYRDWDTDKQPSHEEATQKIMDNLHD
                     GSILLLHAVSKTSTEILNDFISNARKLGYEFELLEY"
BASE COUNT         3681 a         1099 c         1679 g         3451 t
ORIGIN      
        1 taactatgtt aaatatggaa aaatggcaaa acacttgact attaattggt aaaggactat
       61 aataataaat tatcatattg ataaaaacga aaattatgaa atgtctgtaa aaataaaatt
      121 taaataataa ctaggaggag taatatgttt gagaacttga aaaaagcgga ccctgagatt
      181 tatgaaagta tgaaaagaga gttaaaaaga caacaaagaa atattgaatt aatagcatct
      241 gaaaacatag tatcagttcc agtaatggaa actatgggaa gtcatcttac aaataagtat
      301 gcagaaggtt actcaggcaa aagatattat ggtggatgtg aatacataga tgaagttgaa
      361 actcttgcaa tagatagaat caaaaaaata tttggagcag aacatgctaa tgttcaacct
      421 cactctggag caaatgcaaa tattggtgta tattttgcta tgttaaaacc aggtgatgtt
      481 gttatgggaa tgaatttatc tcaaggtgga catttaactc atggtgctcc agttaatatt
      541 tctggtcaat attataaatt ttatgaatat ggtatagata aagacagtgg acttatagat
      601 tttgatgaag ttagaaagat agcacatgaa gtaaaaccta aaatgatagt tgcaggagca
      661 agtgcatatc ctagagaaat agattttaaa aagtttagag agatagctga tgaggttgga
      721 gcattgctta tggtagatat ggctcatata gcaggtcttg tggctgctgg tcttcaccaa
      781 aatccatgtg aagtggctga ttttgttaca acaacaactc acaaaacttt aagaggacct
      841 cgtgggggtg ttattctttg taaagaaaaa tatgctaaag acatagacaa agcaatattc
      901 cctggtattc aaggtggacc tcttgaacat ataattgctt ctaaggcagt ttgctttaaa
      961 gaagctttaa gtgatgagtt taaagaatat caggtacaag ttgctaaaaa tgcaaaagcc
     1021 ttagctgaag aacttattaa aagagatttt aaattgattt ctggaggtac agataatcat
     1081 ttaatactat tagatttgac taataaaaat ataactggta aagcagcaga aaagagattg
     1141 gatgatgctt atataacagc taataaaaat acaattccat ttgacccaaa tggtgctctt
     1201 gttacaagtg gaataagact tggtactcca gctgtaacta ctagaggtat gaaagaagaa
     1261 gatatggcaa taatcgctga ggctatagac ctatgtttaa cttatgatga agaatctaag
     1321 gctagaacat tggttgttgg tttaactgaa aaatatcctt tatatgaaga atataatatt
     1381 atggattaat tataaagaac aactaaataa aatacattta aataattaca atgattgtgt
     1441 atgtatttga atatatttag atataaaaaa ggtgtctcaa aatgaacttt ttagtttatg
     1501 aggcactttt ttgtatggaa tcgaacatat ttgtattatt taaataataa aaaatatggt
     1561 tatctctatt tttagatggt ctctttgtat tttttaatta aaactttaag atttcttaag
     1621 aattatacta ggtttttcta aagatttaaa gtctatactt ataagtatag taatcatatg
     1681 aaaagaaaag ctttaacgag gagggaatct tatgaaggta ttaattctta caggaaaatt
     1741 tggtatgggt cattactcag catcaaattc tttaagtgaa gatataaaag caaaatttga
     1801 taattcagaa ataataatta aagatatatt tgagtacatt atgcctaact attcagataa
     1861 aatgtacaag actttttcta ttcttgtaaa tcggggaagt agtctttata atttatttta
     1921 taaatgtgct gaaaatggaa agaaagatat aaaatttact ttttcagatt attttttaaa
     1981 taaattagat acattacttc atgaagtaca accaacagta gttatttcaa cttttccatt
     2041 ttgttctcaa ttagtatcaa gatataagga aaaatataac tcaaatcttc ctttaataac
     2101 atgtataact gatataagta gtcactcaga atggattagt aaaaatacag actgttattt
     2161 agtagctagt aaatctacta aggaagaact tgtttttaaa ggaatagatg aatctaagat
     2221 taaagttaat ggtattccag taaaaaaaga atttaaaaga atagaacatg taaatcattc
     2281 aactaaaaaa aatattctta taatgggtgg aggattagga ttactgccta aatcagaaca
     2341 attttacaaa gaattaaata gtttagaagg agtaaaaact actgtaataa ctggtaataa
     2401 taaaaaaatg tactataaac tatatggtag atatgaaaat atagaggtag taggttacac
     2461 caatgaagta tataaatata tgaaagattc tgaccttatt atttctaaac caggtggaat
     2521 aacactattt gaaacaattt attcagaact tccaatattg gcatttaatc catttttaca
     2581 acaagagata gataatgcta gttttatatt aaataatgaa attgggagaa tacttggtaa
     2641 aaataaaaaa tactatgtag atgagataaa agatttaatt tatgatgatg ctactcttaa
     2701 agaaatgagc agtaacatga aagaattaaa aaaacaattt gataataata ccttagagaa
     2761 tattttattt tcacttgatg aacaaggagc gtgtagagaa tgtatgtagt aggtttaatt
     2821 atcgttgttg cattgatttt tttagtccat tcaattatac ctacatacta taataaacta
     2881 ttaaataaaa aggtattaaa aaatatggct ggagaaaatg aaattgcact tacatttgat
     2941 gatggtccag ataagagata tacagaaaaa ttactagatg tgttaaaaga aaatgatata
     3001 caagctatgt tttttgtagt agcaaagaat gctgaaaaag agcctgaaat tataaaaaga
     3061 atgttgagag aaaatcatat agttggttta cattcattgg aacacagaaa tgcatggctt
     3121 tatagttata gttatgttaa aaaagatttt attgaaagta caaatataat gaaaaattta
     3181 ggtgtagatg taaactacta tcgtccacca tggggtcata ctaatatatt ttcaaattca
     3241 tttgttaaaa aatataatct aaaaatgact ttgtgggatg taatggcaga agattgggag
     3301 aaagatagta cagtagacat tattataaac aagttaatga gtagaacaaa agaaaattct
     3361 ataatttgtt tacatgatgc aggtgaaaat tcaggtggtg cagtaggtgc acctgagcgt
     3421 actattgaag ctttaaagat agctattcca aaacttaaag ccagtggttt aaagttcgta
     3481 acaccagaaa ggatgtagtt atgataagta aaactgaaaa gaaaaataaa tgcctaggta
     3541 gtgtagcttt tttagtttta ctaatgggca taacaggata ttttgttttt agagggcagt
     3601 cagttgaatc attgataaaa tcattaaaag gtgcaagccc tatgtttata ctaattggat
     3661 ttgtaatgat gtttatatat gttgcctgtg aaggtataaa tatatactta ggtatgaaag
     3721 ctttaaacca aaagactact cttttaaaat gtatgggata tgcttttatt ggattttatt
     3781 ttagttctat tacaccttca gcatcaggtg gtcaaccagc acaagtttat tatatgaaaa
     3841 aagacgatat aaatatatca tattcttcac ttatactatt agtcattgtt gttattcacc
     3901 aagtagttat actggcatat agtggaataa tgttcattat ggaacgtgag tttatattaa
     3961 ataatgtaag tggtatgaac attttactaa tatatggtgt aataacaaat gttgctcttg
     4021 tcattggagt aattgcaatt atattttcaa aaaaacttgt aaacaatttt ataatatcaa
     4081 taacaaattt attaggtaag ttaagaataa taaaagatgt tgaaagttca agaaaagtta
     4141 taatatctca aattgaagag tatgttaaag gtgctcaata tataaagcag aatccaaaac
     4201 ttgtagtcca aattttagtc ataactattg tacaaataac agctatgttt ttagttcctt
     4261 tctttgtata caaagctttt catctatcaa catatactgt atttgagata ttagcgatac
     4321 aatccttgct taatattgct gtttcttcat taccattgcc aggggctgtt ggagcttctg
     4381 aaaatagttt tatgacatta tttaagatat ttttccctgg tcacttatta gttccggcaa
     4441 tgttacttag tagggggata agtttttatg catttgtagc tataagtgga ttgatatgta
     4501 tagtagtaca tgtaaaatct tctgaaaaag taaaagcagt gaaaaaagtt gcttatgtaa
     4561 gataatatta tatagattat ttttattaat atgtcaattt gtgtgtatat tttaattaag
     4621 ctttaaacat gtttacagga gtagaaacta ttaaaacgtg tgacattgct aagcttttgc
     4681 taataaagta taatatttta tatagtttga aatatttaag aaatatagca aaaaaaggag
     4741 cttggtgatg aataaagaga ttccaaaaaa aattttagta gctgatgata atcctgagat
     4801 tagggaaatc gtagaaatac ttttaactgg tgaaggttat gaagttatta tggcaacgaa
     4861 tggagaagaa gctgtaaact tagtagatag tacgattgat ttgattattt tggatgttgt
     4921 aatgcctgta aaaacaggat ttgttgcatg taatgaaatt agagaaaaaa cgacagcacc
     4981 aatattattt ttaactgcaa aaactcaaga ctctgataaa gttattggat tttcagcagg
     5041 aggagatgac tatttatcaa agcctttttc atattctgaa ctaatttcaa gggttaagtc
     5101 tctacttaga cgttattata tataccaggg aaaggaaaag gaaagagagc caaatatatt
     5161 aaaaacaaaa gagttatgtg tcaatttaga tactcaagat gttacactaa atggagaacc
     5221 tgtgtttttg acaactatag aatatagtat attaattctt atgttgaaaa atagaaaaaa
     5281 agttttttcg tctgaaaatt tatatgaaac tatttgggat gagcagtatt tttatacagc
     5341 aaataataca ataatggttc atattagaaa tttaagaaaa aagttagaaa aagaccaaaa
     5401 aaatccacaa tatataaaaa ccgcatgggg aaaggggtat tatattgatt aacaaaatta
     5461 agccatctaa gttgggagtt atactattga tatatgtagt aatctcagct gcagtggctt
     5521 tcattttaaa tataccattt tactctgctg ttgaatctat agtaaaagat gtatcagaag
     5581 ggaaagtata ttttaaaaga gaagtaaatc aacttatgga taattttcaa acttatgtga
     5641 ataacaatgg tataagagca gatgatggag atgatatagc aaaatggaat agtgataatt
     5701 ggtttgtttc tatgaagata tataaaggaa ataaggtttt ttatgataca ttatactatc
     5761 ctaatgaagt acctgataag agtgaggaaa atgagtttta tagcagtccc atggctagaa
     5821 actatgagat acaattttct gatattaaag ctaatatagt tgtagtagct ttatttagat
     5881 ttagatttca aaattttata gaagcaattt ctattacatt tatggcactt acatttatta
     5941 taacattttt actgttgttt agtagaaaga taaagtacct aaggaaaata gaaatgggta
     6001 taaagatagt agagagtggc agtttaaagt atagaatccc agtaaaaggt aatgatgaat
     6061 tatcatcact tgcatctagt attaatgaga tgagtcatac attggagaat caacttataa
     6121 aagaaaggga gatgaagaat aaaaataaag atatagtaac ttcgatatca catgatgtta
     6181 gaactccatt aacgtcagta atttgttatt tagatttgat taaagaccat aaatacaata
     6241 gtcctgagca attggaacaa tacataaaaa atgtaaggtc taaagcatat caaataaaag
     6301 atttaacaga tgaccttttt acgcattttg ttaattctgg agaggaagtt tcaaaagggt
     6361 atgaaaaaat aaatgctaat gaatttattt ttcaattact tttagatagt tcatatacat
     6421 tggaggagaa aggatttcat ataacaatag aaaatgaact ttataaccaa tatcatattt
     6481 atgtagatgt tacacaattt agaaggatat ttgataattt atgttctaat ataataaaat
     6541 atgctaggaa agataagcct atagtatttt taattaaagt agaggattct aaattactaa
     6601 ttatacaaaa aaatagagta agtaattttt ctaagaatgt agaaagtttt ggaattggta
     6661 taaagaattg tgagaaaata gttgcatatc ataatggaag cataaatgta aattcagatg
     6721 ataaagaatt tgctattgaa atatctattc cagcaaaaga atcataaaaa tttaaattat
     6781 atattgtgta attgctatta tatattgtgt aattgcttat gtaaaattct atgaagcagg
     6841 aagcatgtga ctttagttat gggatgttca atattgtgta cctctaggca agtaacaagt
     6901 ttacttataa gaccatataa tattattatc ttaaaaaaag gggacataat aatatggttt
     6961 tgacaggagc acattatatt tacattctat ttatggtaat tatattaatt actatgatta
     7021 tgaaaaagga tacaatagta ccatgtatat taggtgtttt ttttatgggg ttgttctttg
     7081 aaaaaaacat ttttggtgct ataacagcag tgtttaactc ttttattata tctttaaatg
     7141 agttgggtcc aattatcttg attatagcta ttatggttgc attatctaaa gccctagaag
     7201 ccaataatgc tatacagtat atggtgagac cattttcgag agttataaaa aattctaata
     7261 cagcattttt tgtaactgga tttgttatgt tagttctttc ttggtttttc tggccaactc
     7321 cagctgttgc cttagttgga gctgtatttt tacctgttgc tatgagagct gggctaccag
     7381 caattggtgt tgcagtagca ttaaatttat ttggtcatgg tcttgcctta tctacagact
     7441 ttgttattca aggagctcca agtataacag ctggtgcagc aggtgttgca gtatcatatg
     7501 ttataaatga tggtatgatt ttgttttggg tgatgggaat cgtaaccata tctatggcat
     7561 tttatacatt aaaaagagat attaacaaag gaatgtttag agaagaacta aaaggttttg
     7621 aaagtgaaga agtagaagag tttaatggaa aatctaaaat agcaactatt ttagtagctc
     7681 tgggatttct tgctgatata attgctatgt atatatttga cctaaaagga ggagatgcat
     7741 cagctctttt gggtggaaca gcagtatttt taataataat tataaatgtt ataaattttg
     7801 gcaaagatag tcttgaaaat gtatgtgaga atataattga tggttttgtg tttgggataa
     7861 aaatatttgg agcaattata ccaatagcag catttttcta tatgggtgaa gttgcaccat
     7921 tgacaggtgt atttggaaaa gtattagcac caggttcaca aggtttatta tcagatatag
     7981 gcatagctct ttctcaaaca ataccactta ataaatttgc cgtatcaggt atagaaactg
     8041 tagtgggagc aataactggg cttgatggtt ctggattttc tgggatttca ttagttggtt
     8101 cacttgcctc tgtatttgga actgctataa atgcaagtgt tggtgcacta gctgcattag
     8161 gacaaatcag tggtatatgg gtaggtggag gctgtctagt tccttggggt cttatttcag
     8221 cagcggcaat ttgtggagtt agcccaatag aattggcaaa gcgaaacttt gtcccagtta
     8281 taaccggatt ggttgtaaca accatagtag caatgtttat tatttaataa atcgaataaa
     8341 tctttaattg tataaattaa aaatagatgt gtagctataa tttattataa ttgttttact
     8401 aattctaagc catacatcta tttttattga tatttttatt agtattcttt aataaaataa
     8461 tatttttcat tatactatac ataacatcta aatataacat ctaaaatact gttatctata
     8521 tttaatatct tgccaaattt tttgatgttt tattgtcctg tcccaccaac atatataagc
     8581 tatcacaagt ttttacctat actatcacca aacaaaaatt ctaattttaa tttattattt
     8641 tataacctat attatattaa ctatattagt ttgtagactt tagatgtaat tataaatatt
     8701 aatactccaa gagttcaaat tcatatccta gttttcttgc atttgatata aaatcattta
     8761 aaatttctgt actagttttg gaaactgcat gtagtaacaa gatagaacca tcatgtagat
     8821 tatccattat tttttgcgtt gcttcttcat gtgatggttg tttatcagta tcccagtctc
     8881 tgtatgcaaa actccaaaat acagtcttgt atcctaaatt tttagtcatt gccaagcttt
     8941 tttctgagta ttttcccata ggaggtctaa aaaatttaac catatctttc ccagttacat
     9001 ctttatataa cttttcaaca tcatataatt cgtcattaaa gttttttaga ttgcttgttt
     9061 ttgttggcat agatggatgg gttttactat gatttccaac tatatgtccc tcagaaacca
     9121 ttctttttac taaatcttta ttttctttta tatatggagt tgtaacaaaa aaaactgctt
     9181 taacctggtt ttgttttaga gtgtcgagta tctttgtagt gtatccattt tcataacctt
     9241 catcaaatgt aagatatagt gttttttgac catcttttgt aggtccattg tatagggcat
     9301 catagtcaga aaatttaaat tcaatgtccg tatttacttc tggtgtttta tgcttattgt
     9361 ttggtatata aaaccaatca agagtagtgt tatctaaaga agatacatct aatttattat
     9421 catcattatt agtagtggct tttgatggtt tatctgttcc gtctgaaact attacattgg
     9481 aagtatcttt accactatct attttttcct tatcttcttg taactgtgtt tccttatttt
     9541 gattttcgtt ttgattgttt tgtgaatttg aacatccaac aataaaaaaa atagaaatag
     9601 aaagtagtgt taaataatgc aatacttttc taaacatttt gaagctcctt tctaaattaa
     9661 taataattgc atataataat aattatatga ttaattacaa ttgcatatag tattattata
     9721 attggaaatt actattaata atagttatat ctttaaaaat aaagtcacaa tagtatgaca
     9781 aagggtgaat aatcaataaa tttaaaaaaa aatgaaaaaa atagttgacc atattatgag
     9841 aagatgttat tataataaat gtgctcaaca agaagtaaca aaataacaag cacaaagaag
     9901 ttaattgaat
//
LOCUS       DAGSRM010000121         9656 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.126, whole genome shotgun sequence.
ACCESSION   DAGSRM010000121 DAGSRM010000000
VERSION     DAGSRM010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9656
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.126"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(147..1340)
                     /locus_tag="MJE33_003291"
     CDS             complement(147..1340)
                     /locus_tag="MJE33_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBY9649207.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(1419..1625)
                     /locus_tag="MJE33_003292"
     CDS             complement(1419..1625)
                     /locus_tag="MJE33_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBY9649208.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            complement(1618..2760)
                     /locus_tag="MJE33_003293"
     CDS             complement(1618..2760)
                     /locus_tag="MJE33_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBY9649209.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            3269..3904
                     /locus_tag="MJE33_003294"
     CDS             3269..3904
                     /locus_tag="MJE33_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY9649210.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGTNIEASDLYLIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNHFDDEDE"
     gene            4395..5327
                     /locus_tag="MJE33_003295"
     CDS             4395..5327
                     /locus_tag="MJE33_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBY9649211.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTEELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5572..5688
                     /locus_tag="MJE33_003296"
     CDS             5572..5688
                     /locus_tag="MJE33_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBY9649212.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5704..7623
                     /gene="tet(M)"
                     /locus_tag="MJE33_003297"
     CDS             5704..7623
                     /gene="tet(M)"
                     /locus_tag="MJE33_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBY9649213.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGIVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            7742..7909
                     /locus_tag="MJE33_003298"
     CDS             7742..7909
                     /locus_tag="MJE33_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBY9649214.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(7969..8322)
                     /locus_tag="MJE33_003299"
     CDS             complement(7969..8322)
                     /locus_tag="MJE33_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY9649215.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            8827..9246
                     /locus_tag="MJE33_003300"
     CDS             8827..9246
                     /locus_tag="MJE33_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBY9649216.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSCDCIFFKVLNYTIQVSNEKLAVALYKLSSKERDVILLRYFQ
                     SMSDQEIAELYHVSRSAIYRRRSNGLKKLKIVLKERN"
     gene            9288..9491
                     /locus_tag="MJE33_003301"
     CDS             9288..9491
                     /locus_tag="MJE33_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018595044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY9649217.1"
                     /translation="MCQASAGSETAIREILKFYDAYICKLCLRPFYHSESGKITMQVD
                     EELKGQIHTEMMKAILKFEIRVK"
BASE COUNT         3026 a         1717 c         1866 g         3047 t
ORIGIN      
        1 ctatcttttt atatcttctc aaatgtccgc aaagccttat tttatcggct ctacgggcat
       61 tttgcttttg tggtaaacct cgcatatcta ggtctatctt cttatatttt ccctgtcaat
      121 cgtggttaaa atcgtggtaa atacttctat gcgattagac gctgaacctc tgattttgcg
      181 gtatctatgg acgcatgagc gtaccagttc attgtgatac tgatgtttga atgccccatg
      241 atatactgta aatctttcgg gttcatgttc ttgcttgcca gccttgtgca gaatgtatgg
      301 cgtagcgtat gcggtgtaat atgtggcaag ggattgtcct tgtggtgctt gttgtatttc
      361 tttaccatac ggataaataa ggcgttataa tcaatggcaa cttttggatt acctttcgga
      421 ttgataaaca caaaaccgct gtgtccgtct acctctatcg actttatctt tggtcggctg
      481 ttgataaccc gttgaaatgc ctgtatcgtt tctttgctta atggcacttg ccttatcccg
      541 cttttcgtct taggcgtttc aatataatag ccctgttcct tactctttag taactggtgg
      601 tcgataatca caacttcatt cttgaaatca atgtcggcta ctgtcagtcc gcacagttcc
      661 gagatacgaa gtcctgtctt taacagtatc agcacatcat cataatgctt gtgatacacg
      721 ttgtccgtct taataaatga caataaggct tgctcctgtt cctctgtcaa tgcgactttc
      781 tctttggtat cattttctat gacttcactt agcttgaaat caaaagggtt tttcctcaca
      841 taatcatctt gtatggctat gtagaatgac gcttttaacg agcgtttatg gttgttaatg
      901 gtattgtagg aaaagccttt gtctttcatg cgtaacgccc attctttagc gtcagagggt
      961 tttatcgtat caatgctcct agcacctaac ttgtcctctt ttaataaccg catgagttgt
     1021 tcccgttgtt tctgtgtgct tttcttcaca tttgtcctct gtgcgttctg tttggcatag
     1081 agttggcaaa gcgtcatttt cttgcctgtg ctgtcgatac cgtcctcaat atcccgtctt
     1141 atctgctgtt ccagttctcg aagtgagggt ttttcccgtt ttccctttgg tgtcgggtct
     1201 gtgggtgtca atctccaagc atacacatat tttgtgtttc ccaatgcgtc tacatattta
     1261 tataagtatt ttccgtctgt tcgttggctc tctccagtat gcaggatacg acctttgtta
     1321 tcccgtcttt tttctttcat ggtgtctgct cctttccttg ttggaaagag ccttgatatg
     1381 acttgtgttc atcataacac atacaaggct catttgcatt agattgcgtc caatttatca
     1441 ataacctttt caaactgtcg gcgtttaatc tgtatgcggt tgccattcat aatgagccag
     1501 ccagcgtcct tgttttcctc tgccaatcgt cttaacttgt tttctccaat gcggaaatac
     1561 tttgacgctt cttcaatggt aagggtgtat ttttcccata cgggaatatc ctgtgtatta
     1621 ctcataaaac accccctcac tttcgattac gtctgatatg tccgtacaca tctgttccac
     1681 aagcatttcc tgtttttcag aaaggtgggt acattcatca atcattttca tgtagtttga
     1741 acctgtcagc ctgtcgattt cctctatcat cacgattgac ggcatgacct gtctttccag
     1801 ccattgcaga gtgcgttcca tattgacagg tacagggttc atttcaaaag gtaaactgtc
     1861 atgggaaatg aatatctccc acaatggata gtcaggaaat tccacaaaat cggtaatgag
     1921 ataaaacacc agcccatgag ggttatacgt caataacagt tcttcaactg cctgtaccgc
     1981 ctttttgtca cgcagacgaa tttcaaaacg gttgataata tcggtatgtt tctttttcgt
     2041 tgcctgttcc ttttgttttt catacaggca gaaatatttt gtgcttgcct ttgagccgat
     2101 ataaagggta cttgctaaat tacggttttt tccgcttaat tttccacctt gtacattttc
     2161 atagttttta cagcgacatt ccgcaccacc atttctgtat ttttcagata gcacaggaat
     2221 atccagcaga ccgcacatat cattgattgc caagtcaaaa cgtcggataa caccgccaca
     2281 gtcaagacag cggtttaaga atgaatacca gtccctgttc tgtgcctgta agacatattc
     2341 catgttgcgt gagcctgccc cttttaattc taaaaatacg cccatgtgtt catcatcaga
     2401 agccatgacc ttgatttccc cgtaagcata atgctcctta tagccataat acccatagtc
     2461 ataatggata aaataatctg ctttcatgcc aagcaccttt cggataactt ccaatgcgtc
     2521 agtcgttgga aaacggacag acagataatc aacgagcaga taaaaaggct tatcgcagat
     2581 atagtaattc agacaacgca ggataatgtt ctgtgtttcc ttgctggcgt tagcttttcc
     2641 gttctcaaag cggttgatac tatatcttga tacatgggta agacttgcaa gttctccttg
     2701 tgaaagaccg ctggcaagac gtttgttctt catttcaagt gcaaattcta aattgttcat
     2761 tgtattcctc caatcccaca atagccttga aatacctgta aaccaccttt caaaaagtgt
     2821 gatatgtact atcttcttaa aacctctcat tactactcac aggtttgggg gtgtatggtt
     2881 tttgacttgt cttttgtgaa atagtgcccc cctgttagat aacgggggca cagtcaaggc
     2941 tcgcaggctc gccagccgac aaagcatttc cgtcctttga cggcaagccg tcatggcgtt
     3001 gttgctgacg cacgccagca gtcctacaat gctttgtcag ccagtcctgt ttttgtcttt
     3061 ctaacaagtt ccattttaac caatattcat atctacgggt ttaatcagat aggtgtttct
     3121 ttttgaatat ttttgtattt cattctattg tgcttattta attgcaagaa catagtaatt
     3181 cttatatgct tatttgtcaa tatctttttt tgcctttaca aatcatatta agcatgatag
     3241 acttataacc gcgaagatat aacgagatat ggagggttat gcaatgaaaa taagccattt
     3301 aggaaataat atacagacca taagaaaatt tagaggaatg aaacaacagg aacttgcgga
     3361 caaaatcggt atcaatatgc agagcctttc caagattgaa cgaggattga attatcctgc
     3421 ttatgaaacg ctggaaaaga taatggaagt gctggacgtt acgccgaatg aattgttgtc
     3481 gggggaatgg aaatatgtca atcaatccga aaaggaagtc tgccagtttt taagagcaga
     3541 agaacgacta aatgcagagc tgaaacatgg acactatgat aacttctttg acagcgagga
     3601 agaatggctg gaatatgagt tagaaaagtt acgggaatac attaccgact acatcaatgg
     3661 aacaaacatt gaagcgtctg acctttacct aatcaaagaa tttatccagc atttaaagtt
     3721 ccaaaaacta ctagaccgct atgatgattt atacagcatg gatatgtttg gggaaagcat
     3781 agagggacat aagtataaga caccttatca agtcgtgaaa atgataaacc caaattcaaa
     3841 agaagatatg gaactgttac gagaagtatt aaggaataac cattttgatg atgaggacga
     3901 gtaatctttt tcctcttgca agaagataca gaaaacaaaa agcccagtaa agagtgcaaa
     3961 gcaccaccaa tggtggcaag ctcttgacag ggctttttct tttctgtggt tggtaatcaa
     4021 gaggaagaaa gggataaaac ctaatttatg ataaatcaga caacacaact agcaagtttg
     4081 agcttgtgct acacgtgcca taaagtaaca cacaaggatt gatagatacc agcccactat
     4141 gccctaagtt ttattatgga agtaaacaat taacgatatt ttaacgcttg tgtattcttt
     4201 tgagcgaatg ttattagcgg ttgacagatt tccacctctt attggtagaa tgttaaacct
     4261 cgattttggt ataattaaac cacaataaaa aagcaaaaag gagtgctgaa tatgaatttt
     4321 aacgaaaaat tgatttcttt gagaaaaacc aaaggattat cccaagaaga actgggagca
     4381 gaacgaatca aacaatgaga aaggaagatt taatgatgaa atttagaaaa aatcagaata
     4441 aagaaaaaca gataccaaag gaaaagaaac ctcgtgtcta taaggtcaat cctcataaaa
     4501 aggttgtgat tgccttgtgg gtacttttag ggcttagttt cagctttgcg atattcaagc
     4561 actttacagc tatagatact catactattc acgaaacaac tatcatagaa aaggaatacg
     4621 ttgatactca tcatgtagaa aattttgtag agaactttgc gaaagtctac tattcatggg
     4681 agcaatccga taagtccatt gataatcgaa tggaaagtct aaaaggctat ctgacagagg
     4741 aacttcaagc tctcaatgtt gatacagtac gcaaagatat tcctgtatcg tcttctgtaa
     4801 gaggatttca gatatggacg gtagagccaa ctggcgacaa tgagtttaat gtaacctaca
     4861 gtgtagacca gctcattaca gagggagaaa atacaaagac cgtccactct gcttatatag
     4921 tgagtgtcta tgtagatggt tctggaaata tggtactggt taagaatccg accattacca
     4981 acatacctaa gaaatcaagt tataaaccaa aagccattga aagtgagggg acggttgatt
     5041 ccattacaac caatgaaatc aatgagtttt taacgacgtt cttcaagctc tatcctacag
     5101 cgacagccag tgaactttcc tactatgtga atgacgggat attaaaacca atcggaaaag
     5161 agtacatctt tcaagaactg gtaaatccta ttcacaatcg taaggataat caagtcacgg
     5221 tatcgctgac agtggagtat atcgaccagc agaccaaagc aacgcaggta tctcaatttg
     5281 atttggtact tgaaaagaac gggagtaatt ggaagattat agaataacaa atattggtac
     5341 attattacag ctattttgta atcacgtact ctctttgata aaaaattgga gattccttta
     5401 caaatatgct cttacgtgct attatttaag tatctattta aaaggagtta ataaatatgc
     5461 ggcaaggtat tcttaaataa actgtcaatt tgatagtggg aacaaataat tggatgtcct
     5521 tttttaggag ggcttagttt tttgtaccca gtttaagaat acctttatca tgtgattcta
     5581 aagtatccgg agaatatctg tatgctttgt atgcctatgg ttatgcataa aaatcccagt
     5641 gataaaagta tttatcactg ggatttttat gcccttttgg gtttttgaat ggaggaaaat
     5701 cacatgaaaa ttattaatat tggagtttta gctcatgttg atgcaggaaa aactacctta
     5761 acagaaagct tattatataa cagtggagcg attacagaat taggaagcgt ggacaaaggt
     5821 acaacgagga cggataatac gcttttagaa cgtcagagag gaattacaat tcagacagga
     5881 ataacctctt ttcagtggga aaatacgaag gtgaacatca tagacacgcc aggacatatg
     5941 gatttcttag cagaagtata tcgttcatta tcagttttag atggggcaat tctactgatt
     6001 tctgcaaaag atggcgtaca agcacaaact cgtatattat ttcatgcact taggaaaatg
     6061 gggattccca caatcttttt tatcaataag attgaccaaa atggaattga tttatcaacg
     6121 gtttatcagg atattaaaga gaaactttct gccgaaattg taatcaaaca gaaggtagaa
     6181 ctgtatccta atatgtgtgt gacgaacttt accgaatctg aacaatggga tacggtaata
     6241 gagggaaacg atgacctttt agagaaatat atgtccggta aatcattaga agcattggaa
     6301 ctcgaacaag aggaaagcat aagatttcag aattgttctc tgttccctct ttatcatgga
     6361 agtgcaaaaa gtaatatagg gattgataac cttatagaag ttattactaa taaattttat
     6421 tcatcaacac atcgaggtcc gtctgaactt tgcggaattg ttttcaaaat tgaatataca
     6481 aaaaaaagac aacgtcttgc atatatacgc ctttatagtg gagtactaca tttacgagat
     6541 tcggttagag tatcagaaaa agaaaaaata aaagttacag aaatgtatac ttcaataaat
     6601 ggtgaattat gtaagattga tagagcttat tctggagaaa ttgttatttt gcaaaatgag
     6661 tttttgaagt taaatagtgt tcttggagat acaaaactat tgccacagag aaaaaagatt
     6721 gaaaatccgc accctctact acaaacaact gttgaaccga gtaaacctga acagagagaa
     6781 atgttgcttg atgccctttt ggaaatctca gatagtgatc cgcttctacg atattacgtg
     6841 gattctacga cacatgaaat tatactttct ttcttaggga aagtacaaat ggaagtgatt
     6901 agtgcactgt tgcaagaaaa gtatcatgtg gagatagaac taaaagagcc tacagtcatt
     6961 tatatggaga gaccgttaaa aaatgcagaa tataccattc acatcgaagt gccgccaaat
     7021 cctttctggg cttccattgg tttatctgta tcaccgcttc cgttgggaag tggaatgcag
     7081 tatgagagct cggtttctct tggatactta aatcaatcgt ttcaaaatgc agttatggag
     7141 gggatacgct atggctgtga acaaggattg tatggttgga atgtgacgga ctgtaaaatc
     7201 tgttttaagt atggcttata ctatagccct gttagtaccc cagcagattt tcggatgctt
     7261 gctcctattg tattggaaca agtcttaaaa aaagctggaa cagaattgtt agagccatat
     7321 cttagtttta aaatttatgc gccacaggaa tatctttcac gagcatacaa cgatgctcct
     7381 aaatattgtg cgaacatcgt agacactcaa ttgaaaaata atgaggtcat tcttagtgga
     7441 gaaatccctg ctcggtgtat tcaagaatat cgtagtgatt taactttctt tacaaatgga
     7501 cgtagtgttt gtttaacaga gttaaaaggg taccatgtta ctaccggtga acctgtttgc
     7561 cagccccgtc gtccaaatag tcggatagat aaagtacgat atatgttcaa taaaataact
     7621 tagtgtattt tatgttgtta tataaatatg gtttcttgtt aaataagatg aaatattttt
     7681 taataaagat ttgaattaaa gtgtaaagga ggagatagtt attataaact acaagtggat
     7741 attgtgtcct gtatgtggaa ataaaacacg attaaagata agggaagata ctgaattaaa
     7801 aaaattcccc ctctattgtc cgaaatgcag acaagaaaat ttaattgaaa taaagcagtt
     7861 caaagtaact gtgattacag agccagacgc aaagacgcag agccgataaa atgagattaa
     7921 tacaatctca ttttatcggc tctttccgtt atgtatggat tcttttaatt agtcttcgat
     7981 gtttcttgct tcgttgatac cgctggctaa agattccatt aaggatagtt ctttgtctgt
     8041 aaagctatcc atgtatttct ctatctgtaa tcgtcgggtg ctttttacca agttattagc
     8101 aggtaagaaa aattcatcaa cggaaacatg aagtaacgat acaaggtcat aaagaacttg
     8161 tatgctgggg tgttgccctt tattttcaat attagttaag taccgtgggt caatttcaat
     8221 caatgctccc acttgttcac gagttaaacc tcgtttcaat cgagcttctt taatggctaa
     8281 accaaaggct ctaaaatcat atttatcttc ttttttacgc atagtagacc acctctatac
     8341 attttattgt tcctactgaa ttaaaaacag gtatagaaaa acgtgttata tggtttatag
     8401 gtttatattt aataaaaagc actactaaac gccaataaaa aaaaccgtta tatggtagtg
     8461 ctatttacgc tgttaaaata ttgtatatta cttccaaatg gcggtttgtt ggaggtcaac
     8521 gtcgccatga agtacatcat atacaataaa tttccttaca ttgggttctt gtcaaaaaaa
     8581 gtcgtctatc tgcaatagat aagtacgtcc accaatgtgg ttttataaat catatagata
     8641 gaataacaga agcatgtaaa cagagaaata aatctgttta tatgcttttt tggctattca
     8701 gaactttttt acaaagttta tttatcagta atgcaacaaa tccccctttc acattgggac
     8761 taagagtgaa aggagataaa cgagcaaggc tcacttcctt tcctagacag aaagggggtg
     8821 agaaacatga aaccatcttc ttttcagacc acaatagaaa atcagtttga ctatatctgt
     8881 aaacgtgcta tggaagacga gcgaaagaat tatatgcttt atctttcaag gattgcaaag
     8941 cgtgaggtgt ccttttcgga tgttggcgat tatcttgtta gccagtttgc gacaacagat
     9001 aactattcct gtgattgcat tttttttaaa gtcttgaatt atacaatcca agtatcaaat
     9061 gagaaacttg ctgttgcttt atataagtta tcgagtaagg agcgtgatgt aattctatta
     9121 cgctatttcc aaagcatgag cgaccaagaa atcgcagaat tataccatgt atcacgttct
     9181 gctatttatc gcagaagaag taatggattg aagaaattaa aaatagtatt gaaagaaagg
     9241 aattgaacca atgaaaaaaa gaatatggtt accctacatt aaaagtaata tgtcaagcgt
     9301 ctgctggaag cgaaactgcc ataagagaga ttttaaaatt ttatgacgct tatatttgca
     9361 agttatgctt gcgtccgttt taccactctg aaagtggtaa aatcactatg caggttgatg
     9421 aagaactaaa aggtcaaatt catacagaaa tgatgaaagc aatcttgaaa tttgaaatca
     9481 gagtgaagta atcatattac atgaatgaaa aacgggagat acacagatgt ttgtgctatc
     9541 tcccatgtta ttttttgtgt catatcaagg ctcattcaat cgtggtaaat tcgtggtaaa
     9601 atgagtgttt ttctatactt caaaatgttt gaaagtatag tgttcatgcg gataat
//
LOCUS       DAGSRM010000122         9528 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.127, whole genome shotgun sequence.
ACCESSION   DAGSRM010000122 DAGSRM010000000
VERSION     DAGSRM010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9528
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.127"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            406..1683
                     /gene="hflX"
                     /locus_tag="MJE33_003302"
     CDS             406..1683
                     /gene="hflX"
                     /locus_tag="MJE33_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBY9649218.1"
                     /translation="MDMAKKGITVGLNINNKSEDFNELMIELENLCSACDIDVVGSIT
                     QNAKQVNRAFYIGTGKVEEILNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRT
                     ALILDIFAQRAKTREAKLQVELASLKYMLPRLIGSNENLGRQSGGVGTKNRGSGEKKL
                     ELDRRRIEEKITSLNRELDDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLV
                     DIFKNSEEKKVFEKNMLFATLETSVRNIVLANNKEFLLSDTVGFVSNLPHDLVKAFRS
                     TLEEACEADVLLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVEVL
                     DRILDSIDKEGIFVSVKKDINIDKMIKCICDSIFKDYVRYKFLIPYDKGHVVSYFNEN
                     TSIINTEYREDGAILDVECSNIEYNKYKKYALE"
     gene            1916..2413
                     /locus_tag="MJE33_003303"
     CDS             1916..2413
                     /locus_tag="MJE33_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBY9649219.1"
                     /translation="MDAESLMKKYMVDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIEIEQNEKDSRAI
                     CLVTKDRVQEYSKKVRGMQEKVLQLLFEDFTEKEAEQLFFGIMKLSIGIENVKNYVEN
                     NDVGE"
     gene            2462..2986
                     /locus_tag="MJE33_003304"
     CDS             2462..2986
                     /locus_tag="MJE33_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBY9649220.1"
                     /translation="MKSVVVIYKSKYGSTKKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIILGGGLYACNIAGISFLKKNYKELKKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKIPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPAEAPLLSGEAFDWCN
                     KENLTPILDYIREL"
     gene            3298..4662
                     /locus_tag="MJE33_003305"
     CDS             3298..4662
                     /locus_tag="MJE33_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBY9649221.1"
                     /translation="MDIKELVKMQRKYYNTGKTRDISFRIEQLKKLKLVVSQNEEKIL
                     LALKKDLNKSDFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKVVPQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLAAGNCVILKPSEYSVSTSSVVKDIVEDTFSKE
                     YVAVVQGSQEESEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCII
                     LKDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNPC
                     NNEQFPKIINQRHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYKDLDEVIQKIIQMPNPLALYIFTKNKYLENKLLEMISAGGCCINDTVT
                     HIATNYLPFGGIGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKVL
                     KKIM"
     gene            4801..5967
                     /locus_tag="MJE33_003306"
     CDS             4801..5967
                     /locus_tag="MJE33_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBY9649222.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVMFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVESKLDKKGLPTDTSDYM"
     gene            6084..6866
                     /locus_tag="MJE33_003307"
     CDS             6084..6866
                     /locus_tag="MJE33_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBY9649223.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYADSLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(6985..7638)
                     /locus_tag="MJE33_003308"
     CDS             complement(6985..7638)
                     /locus_tag="MJE33_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY9649224.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(7662..9026)
                     /locus_tag="MJE33_003309"
     CDS             complement(7662..9026)
                     /locus_tag="MJE33_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HBY9649225.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSVFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
BASE COUNT         3743 a         1048 c         1490 g         3247 t
ORIGIN      
        1 aagtatatgt ttttattact tttattaatc atgcaaataa ctacttaaat ttaagaatag
       61 ggttaaatat aaaatgataa ttacatataa gttaattaaa ctaatgtatt tttaatatat
      121 atttagaatt ttaatccttc ttatgatata agtagcttgt tttgtattac ttagatttgt
      181 aaggagtttt tttattgaaa aaactgcata atatatttgg gaggaggtgt aagaaagtga
      241 ttttttatat aagattgaga aaataatgca cgattatggt tattataata gtaaataaga
      301 agtgtcattt aaggataaga taaaaagcaa aattcttgat gaatttaatt atagagccat
      361 aatctcgtgc tatagattaa ataaaaaata gcagataagg agaatatgga tatggcgaaa
      421 aaaggaataa cagtaggatt aaacataaat aataaatctg aggactttaa tgaactaatg
      481 attgaacttg aaaacttatg ttcagcatgt gatattgatg ttgtagggag tataactcaa
      541 aatgcaaagc aggttaatag agcattttac attgggacag gtaaagttga ggaaatatta
      601 aacttaataa agaaagaaaa tatagagata gttatatttt acaatgaact ttctacatca
      661 caactaaaaa atcttgaaga aaagcttaat tgtgaaatta tagatagaac ggcattgata
      721 cttgatatat ttgcacaaag ggcaaaaact agagaagcta aacttcaagt agaattagca
      781 agtttaaaat atatgttacc aagacttata ggttcaaatg aaaatttagg acgtcaaagt
      841 ggtggagttg gtactaaaaa tagaggttct ggtgagaaaa aattagaact tgaccgtaga
      901 agaatagaag aaaagataac atctttaaat agggagttgg atgatttaaa attccaaaga
      961 gaaactcaaa gaagtatgag aagaaaatca aatcttccaa atgtagctct agttgggtat
     1021 acaaatgctg gtaaatcttc aataatgaat aaactagtag atatatttaa aaactctgaa
     1081 gaaaagaaag tgtttgagaa aaacatgtta tttgctacac ttgaaacttc tgtcagaaat
     1141 atagttcttg caaataacaa agaattttta ttatctgata ctgttggttt tgtaagtaat
     1201 ctaccacatg atttagtaaa agcatttagg tcaacattag aagaagcatg tgaggcagac
     1261 gtactgttac atgttattga tatttcaaat ccaagctata aaagtcatat taaagtgaca
     1321 gaagatactt taaaacaaat aggtgcagat ggtataccta tgatacatgt gtataataaa
     1381 attgatttaa tagatgtaga agtattggat agaattttag atagtatcga taaagaaggt
     1441 atatttgttt cagttaaaaa agatataaat atagataaaa tgataaaatg tatttgtgat
     1501 agcatattta aagattatgt aagatataag ttcttaattc catatgataa aggtcatgta
     1561 gtttcttatt ttaacgaaaa tacgagcatt ataaatacag aatacagaga agatggagct
     1621 attttagatg tagaatgtag caatatagaa tataataagt ataaaaagta tgctttagag
     1681 taaattttga aaaacttatt atagcctaaa atatatttaa tttataattt attataaatt
     1741 gattgtaaat atgattttat gaaacggtat agccataatg ctatgccgtt ttgccattta
     1801 taataaaaat aaatttattg acataatagt aactaattac tataatataa agtaattagt
     1861 tactatttaa aattatcaaa aaattaaaat ctaagataaa gagaagtgta taataatgga
     1921 tgcagaaagt ttaatgaaaa aatatatggt agatgatgat agtagaaaac aagctatatt
     1981 tgcaagtata tttgtaatgc aaaataaact tcaaactaca tgtgataaat tagacccaga
     2041 attaacaatg aaacaatggt tacttttagc tattgcggac tctgtagatg aaactcttac
     2101 attaaccaaa cttggagaat taatggggtg ttcaagacaa aatgtaaaaa aactagctat
     2161 tgctttagaa aagaaaggtt ttatagaaat tgagcaaaat gaaaaagatt caagagctat
     2221 ctgtcttgtg acaaaagata gagtgcaaga gtattctaaa aaagtgagag gtatgcagga
     2281 aaaagtatta cagcttttat ttgaagattt tacagagaaa gaagcggaac agttattttt
     2341 tggaattatg aaattaagta taggaattga aaatgtgaaa aattatgtag aaaataatga
     2401 cgttggagaa taagcaagta tcaaatcaac tttgtatgac atcagagagg ggagaattta
     2461 tatgaaaagt gtagtagtga tttataagag taagtatggt tcaacaaaaa aatatgctaa
     2521 atggctttca gaattattgt cttgtgatat ttttgaaact aagaatatta atatagaaaa
     2581 ggttatgaag tatgatacga ttattttagg aggaggtctt tatgcttgta atatagcggg
     2641 aatttctttc ttaaaaaaaa attataaaga attgaagaaa aaaaatttag tagtatttgg
     2701 cgtaggagct gctccatatg atgatgaagt aataaagaaa ttaagagaag taaactttaa
     2761 ggatgaatta aacaaaattc cttgctttta ttgtcgtggg gcatgggatg agacaaaaat
     2821 gtcttttata gattatactc tttgtagttt ttttaaaaaa tctatagcta aaaaagaacg
     2881 tcaaggtctt aaaccagcag aagcaccttt attgtctgga gaagcctttg attggtgtaa
     2941 taaagaaaat ttaactccaa tacttgatta tataagagaa ctttaagagg tatatatttg
     3001 taattattta ttatctaaag ttagatttca tagaaattta ctagacatta tttataaaat
     3061 aatattatta aagatggata atttaattgt ttcaataagg ttataatttt aaaaaatgtt
     3121 tattttaaaa taaattattt tataagctta cttatactta ataaggggct ttttttattg
     3181 gaagatttat gttatattga gatgaattta ttatgatata aaaaataatg ttaggataaa
     3241 tttaaatctt tttctaaaaa tgtattaata taattcttaa agaaaggatt tttaataatg
     3301 gatataaaag agttagttaa aatgcaaaga aaatattata atacaggtaa aacaagagat
     3361 atatcgtttc gtatagaaca gttgaagaaa cttaagttag ttgtatcaca aaatgaagaa
     3421 aaaatacttt tagcactaaa gaaagattta aataaatcgg attttgaagg ttttatgaca
     3481 gaagtaggca tgttttatag tgaattaaac tttgctataa agaatataag aaaatggagc
     3541 aaaataaaaa gagttaaatc aagtatggtt aattttccat ctatatccaa agttgttcca
     3601 caaccttatg gtgttactct cataatgtca ccttggaatt atccatttca attagcactt
     3661 ataccattag tttggtcatt agcagcaggg aattgtgtta tattaaaacc ttctgaatat
     3721 tctgtgtcaa catctagtgt agttaaagac atagtggaag atactttttc aaaagaatat
     3781 gttgcagttg ttcaaggaag tcaagaagaa agtgaaaagt tactattgga aagatttgat
     3841 tatatatttt ttacaggaag tactaatgtt ggtaaaataa tcatgaaatc agcatcagag
     3901 catttaactc ctattacttt agaacttgga ggaaaaagtc cttgtatcat tttaaaagat
     3961 gctaatatag accttactgc caaaagactt acttggggaa aactaataaa tgcaggacaa
     4021 acctgcgtgg ctccagatta tgttttagtg catgaagata gaaaaaatga gttaatagag
     4081 aaaataaaat attatactaa taagtatttt ggagataatc catgtaataa tgagcaattt
     4141 cctaaaatca taaatcagag acattttaat cgaatattat ctcttattga taaagataag
     4201 atagtctatg gtggaaatta taataaggag acattaaaga tagagccaac tatagtagac
     4261 aatgttaatt gggatgataa tataatgaag gaagaaatat ttgggcctat atttcccatt
     4321 ttaacatata aagatttgga tgaagtaata caaaaaatta tacaaatgcc aaatccattg
     4381 gcactttata tttttacaaa aaataagtac ctagaaaata aacttttaga aatgatttca
     4441 gcaggaggat gttgtatcaa tgatacagtt actcatatag ctacgaatta cttacctttt
     4501 ggtggtattg gtgaaagtgg tatgggctca tatcatggaa aagctggatt cgatacattt
     4561 actcactata agagtgtgtt aaaaaaatta aatttggatg tccctattag atatgctcca
     4621 tacgataata aattaattaa ggttcttaaa aagataatgt aggttatatt gtagaataat
     4681 aaatataaat aaaattgagt atttgtattc tgaatatatt ttatttttaa tttggtatat
     4741 ttgttataaa agtggtgatt ttgtgataaa atttaattat atagaaataa ggaggaaaaa
     4801 atgaaaatta attggaatac aaaatacaca acaatagcaa tttatacatt tataatagct
     4861 gctagtagta ttatatttta tctagtatca tcacaaattg atgtattttc taataattta
     4921 gatgctatat ttacaacatt acaacctttt ataataggat ttgctattgc atatcttctt
     4981 aattttattt taaagtttta tgaagacaga atttttataa aaagtgagaa attaaaaaaa
     5041 ctaaaacaaa gttcaaaaag aggcttgggt cttttattaa catatgcaac agctacattg
     5101 atactttact tatttatgca tttcgtattg ccacaagtaa ttgagagtat tgttggacta
     5161 gcaaatgata tacctatgta tgtaaacaat gcaactaagc ttattgataa attaatgact
     5221 gatttaaatc ttgatgagca atactttaat ttagcagttg ataagtggaa tgagtttgtt
     5281 acatatatta taaagtttgt aacagattta atcccaatat taggaagtat gttaaagaat
     5341 gtagcctcta gtatatggaa tattgtacta ggattaattg tttcagtata cttattaatt
     5401 gataaagaaa aattctatgg attaagcaaa aaaataactt atgcagtatt tacagaaaaa
     5461 caagctgcta gaattttaga attaactcat agaagtaact atacatttgg aagattttta
     5521 ggtggcaaaa tattagattc attcattata ggtattctaa catttgtaat acttacatta
     5581 gttaaaatgc catatacatt attaatatca gttataattg gtattacaaa tattatccct
     5641 ttctttggac ctttatttgg agctatacca tccacgctaa tagtgatgtt tgtatctcct
     5701 ataaaagctt tctggttatt gttaattata ttaataatac aacagataga tggaaacatt
     5761 ataggaccta aaatacttgg agattcaatt ggtatatctg cattttggat tttgttctcc
     5821 ttattggttg caggaaaatt attaggtttt attggaatgg ttataggagt tccaatgttt
     5881 gcagtaattt attctataat taaggatatt gttgaaagta aattggacaa aaagggatta
     5941 ccgacagata cttccgatta tatgtagcta aaaaatatta tgtataatta aaaatatata
     6001 aattaagcaa tatttaaagc actgagaaag ttaaactctt ggtgcttttt agatataaaa
     6061 ttattataga agaggtgttt tttgtggcat ttatagaatg taaatttaaa tctaaatata
     6121 ctggaggaga aactgatata attgtaattt tgcctgtagc aaatggaaaa gaacttttta
     6181 gtggtaaaga gatatatgat tttgaaggta aatttaaaac attatactta tttcatggtt
     6241 tagaagatga ccaaagctca tggattagat atactaatgt agagcattat gctaaaaata
     6301 aaaatatagc cctagtaata ccaagagtag aaactagttt ttataccgat atggcgtatg
     6361 gacaccaata ttttactttt gtaagcgagg aattacctaa atttgttaga gctatatttc
     6421 ctttatcaga taaaagggaa gataactttg tagctggtat gtctatgggg ggatatggag
     6481 ctttaaaatt agcgctttca aaaccaaaag agttttcggc agttgcaagt ttttctggtt
     6541 cgcctgatat gattcatgag ttagaaaatc aacaaataga aaccaatgca gacattctat
     6601 ttcacgattt tggtagacca gaagacgcta gaaatagtga gaatgattta ctttacatac
     6661 ttaagaacct taaagaaaga aatgaagata ttcctaaaat ttatcaattt tgtggtgata
     6721 gagatttttt atataaaaat aatcagatgt ttaaaaaata tgcagattct ctgggtgtag
     6781 atgttgaata tgaagagtgt gtaggggggc atgaatggcg tctttggaat ttatggataa
     6841 agaaatttat agatataatt ggatagtaca cttatggtat taacaggtat actataaaat
     6901 atctttaaat atgataaaga ctgaaatcct aaaatcaaat tatgatttta aaatttcagt
     6961 ctctaagaat ttaataaaat tttattaagt tcttacacga gttattattg gagtaaaatt
     7021 gtcaactata tctatatagt atttactcaa ttcctctaaa atctcttcgc ataaatccgt
     7081 actatgacca agattaataa gaaactgttt tgtaaaatgc tttattacag cttgagaaga
     7141 tatatcgtac ttgtgtgaag tataaactct aattatttca ggtttcatag caagtatacc
     7201 aatacaagaa agtattaatt catcatgttc tatagattct ttatcattaa catatctttt
     7261 catgaccatt tcggttacac ttctttgata gcttaaaata gcttcttcat aagctatatc
     7321 caaataaaaa tttctatatg cttcgttttt tagaaggaaa caaaggtcag cattcatagc
     7381 aagaaagaac atcatcatag taccttcttc atggtagtta tagattaaag actgcatttc
     7441 atttcttata tcttcatata ttatagctcc tatttcgctt ttacttgaga agtaatagct
     7501 tattagacct aaatttatat tagctctttc tgcaatttgc ctagttgttg tcttatgata
     7561 accttgctca aaaaataatt ctctagcagc acttataata ctagcttttg tttctttgcc
     7621 cttttctgat tttgacatat ttactgtgta tacctctctt ttcaatcttt ttttgaaaat
     7681 ttgctaacta taaatattgt aatagaacca cctattatag gaactaaaat tctgtcaaat
     7741 acagtaagaa taccaatcat atttaataat gccaatataa cacttactat aacagcatag
     7801 atagctcctt tttttgtagc acctttccag caaagaccaa ttacaaatgg tgcaaatatt
     7861 acacctgtac ataatggagc tggccaaaac aataattcta ataaactatc aaatttaagt
     7921 gctatgattg cacctattat agctgtcaaa gctgatgcaa tttgagaata ctttagagta
     7981 ttttttaaat cattaaattc tatattagga tgtaataatt tgttataaat atctcttgat
     8041 ataatagcac tcattgaggt cattaatgaa tcggcagtag acattatggc agccaatata
     8101 gcagctaaga ataatacaga tattaaaggt ggcaaagtag tcgatatcat ttgaggtaaa
     8161 acctgaacag atgatatatt tggaaataaa ctaccaccaa tagctccaat tattcctata
     8221 gggaaagcaa gaaataatag tataaatcct gcaattacac tacttttaac tgcattttta
     8281 gcattatcag cacattgata tcttaaaaaa ctttcataag aaaatattga gcctaaaact
     8341 aatggtaaaa ctgtatacat aaaatcacta atcgatttat ttcctggaaa gaatggttct
     8401 tttaccattg attgtaacat aagtaaaggg ttgtcgccaa ctaattttaa gcttatataa
     8461 gctactattg gcaaaaacac tatacaaata gcaagttgaa taaatgctga tactgcaact
     8521 ccccacattc ctgatgtata ggtatatata attataacta taactcctat aattacagct
     8581 atatcataag gaataccaaa tgcagtagat acagaaccaa aagaaattaa ttgactaccc
     8641 ataatagcta gtatggatat tggtgttaca agactttgat aagctcttaa aaatacacta
     8701 tgaccatatc tagtttcaat ataatcagca ggtgttatat cacaagataa gcttcttaat
     8761 tttcctgcga aaaatgcatt tataaagcaa gctaaagctg cacctatagt agaatacatt
     8821 ccaacccaaa gtcctctttc agctccccat tgaactccac caactaagca ccctcctcca
     8881 aaagatgtag cagcaatggt tgcagctagt ataggcaaag gcagatttct acctgcgatt
     8941 aggaaatcgg atgctgtttt aacttttttt gaaaaataaa aaccaatcca tatgtatatt
     9001 gcacacgtaa caattaaaag taacatagtc ttttctcctt tataaaatat tttaataaaa
     9061 tatacaaaat atatttaaaa tatattttat ataaaagtag tacgatattt gttttattgg
     9121 ttttagatta atatagctat aataatttat gatattagat ggaaattacg taaaaataga
     9181 aaattgaaaa ttctgaaatt tttgcatatt gatatttatc tcatagattc ttttttgtaa
     9241 ttgtaacacg cttttatttt taaatcaata gtacatgtat aaaaataaaa ggaatttaaa
     9301 taagaaaaag atatttttaa tatctggatg tagtatagaa atatcttgaa taaattattc
     9361 ttgaataaaa ttataaaaaa ttaaaatcac tagaaatatg ataaaataat ttaaaatacg
     9421 catttcaatg acatttcaaa acaagaaaat aataaaaaac aaacctaaat taagtctaaa
     9481 tagcttttta gttaatttta gtagtaaaaa ataaaactta aaaagtat
//
LOCUS       DAGSRM010000123         9295 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.128, whole genome shotgun sequence.
ACCESSION   DAGSRM010000123 DAGSRM010000000
VERSION     DAGSRM010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9295
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.128"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            119..748
                     /locus_tag="MJE33_003310"
     CDS             119..748
                     /locus_tag="MJE33_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649226.1"
                     /translation="MNKYLLINPVAERMYGEKFHLIKANLIKKGYIIAECEPQLEYVK
                     KQYKEYTKKTKNTMLDCRCPESISLLKRNNLINDFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLVVTCPCTQLRDFASEKFKDKGNAMFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGFEDKYDIVEMFYCKDGCNNGDGL"
     gene            749..1531
                     /locus_tag="MJE33_003311"
     CDS             749..1531
                     /locus_tag="MJE33_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9649227.1"
                     /translation="MVNKFNNKFLRLVKSVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTFLNMISDGSIFINVYASMKRVLIGFAISTAIGVPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVEAYE"
     gene            1524..2402
                     /locus_tag="MJE33_003312"
     CDS             1524..2402
                     /locus_tag="MJE33_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9649228.1"
                     /translation="MNNLVCDSGYIIRNLYKSYKVDNEEHVVLSDISLNIDRNHITVI
                     LGESGCGKTTLLRAIASLEGINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     AFHNQRKNTILNKVLRKFKHNKRISSKIINDNFRSLETNDNLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLLMDEPFSALDYFTRIDMQN
                     EVIRIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKIKEFNIEDGYNRDLTSDYYI
                     KLKKDILMTMKSNKGI"
     gene            2435..3394
                     /locus_tag="MJE33_003313"
     CDS             2435..3394
                     /locus_tag="MJE33_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBY9649229.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSVDAALIAGPAALKAMKSGSKLVANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYA
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKELIYNAK"
     gene            3708..4400
                     /locus_tag="MJE33_003314"
     CDS             3708..4400
                     /locus_tag="MJE33_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY9649230.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSHIRRVYGEYSPKIDERIVELDGLKFYPERLELELNGENLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     VRGLGYKLNKNW"
     gene            4410..5414
                     /locus_tag="MJE33_003315"
     CDS             4410..5414
                     /locus_tag="MJE33_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY9649231.1"
                     /translation="MKLFLKDYKGYIFIYYIGIVMTIIYCNLMKFISFGESLYILLFS
                     SFILLCFLIYKYYKNREVYNLFEKGVHSLEESFLYLGSSFLGRNISVILKQQHKLYQS
                     MIQEHKKIHSEHLTFINQWIHQMKTPISVISLQLQDYEGEEVTENIRKEIDRLNKGLD
                     MAMHFARADNFQKDFIVEKAKLNEIVSSIVNEEKRLFIKSGMIPKIQVDKFTYVYTDV
                     KWMKFIIGQLITNGVKYSKDYSNYLTIKSEENNKYVILSIIDEGIGIVSKDLKRVFDP
                     FFTGENGRKFGESTGMGLYLVKKVCDDLGHKVSIKSEVGKGTEVSIYFNKDVNSLIKV
                     "
     gene            5662..6513
                     /locus_tag="MJE33_003316"
     CDS             5662..6513
                     /locus_tag="MJE33_003316"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBY9649232.1"
                     /translation="MSLDVIFILKSVIIAIVEGLTEFIPVSSTGHMILVGNLIDFKGQ
                     FAEMFEVVIQLGAILAVVVLYWKKIKDSVIEFFKFIFTGGKEGKIGFRFGMNVIIGCI
                     PFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQA
                     LKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFEF
                     DYSIMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAVYRIIMGVVLAVLA
                     FTNIISV"
     gene            6617..7363
                     /locus_tag="MJE33_003317"
     CDS             6617..7363
                     /locus_tag="MJE33_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9649233.1"
                     /translation="MKILTVNNLSKVYGKKIIFNALNDINFSIEDGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKGKNPLLLRGEELALFRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDERLNEVSTILGIKDLLGKRTFEVSGGQAQRTAIARALINN
                     PSILLADEPTGNLDSKSSKVVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     SIYNEIYKGSSREQFYQEIMDVLTLLGGDN"
     gene            7363..9279
                     /locus_tag="MJE33_003318"
     CDS             7363..9279
                     /locus_tag="MJE33_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9649234.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASIVISSSLLFSFIMFTSHPDL
                     DVSQFPDYLKEGLKMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISKRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVVMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSIFTSFIVKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAITST
                     DQYTTYLRLVPVTSLTIVATYLFFSQFSVFFMKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVERQINLIYPNTIYYSTMTLHNDPKKPD
                     SKEKDKERMMFIENNLKKENVDYTRTNGEFKTVFPKKSDFNVRIMKESKYLEITETIG
                     VKPISFEDNECISLLSSSLPGNKNIKENIIVGNRGLKVAKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICRKFENKFNNESGMQPYLFFSKELMIGT
                     GKLMYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLFPYVVAVIHSIFALTALKNSFDIEVASSAVLVMGSLFLVQIVYFLIVR
                     NNYLKEIRLNLVNF"
BASE COUNT         3611 a          879 c         1651 g         3154 t
ORIGIN      
        1 taaaaaatgt agataaaaat tgttttatta agaaaaaatt aatatgttga caaaatttaa
       61 gtgctcattt aaactattaa ttgataataa tattcaatat taaataatag gaggcgttat
      121 gaataaatat cttctaataa atccagtggc agaaagaatg tatggagaaa agtttcacct
      181 tattaaagca aatttgataa aaaaggggta cataatagca gaatgtgaac cacagttaga
      241 atatgtaaaa aagcagtaca aagagtatac aaaaaagact aaaaatacaa tgttggattg
      301 tagatgtcca gaaagtataa gtcttttaaa aagaaataat ttgataaatg attttgaagt
      361 acctatgata gaaccaatat tagtaaggac ttgtagggtt ttatacgata aatatataaa
      421 aagtaaagat gatatgttgg tagtaacctg tccatgtaca caattaagag actttgcaag
      481 tgaaaagttt aaggataagg gtaatgctat gttttataca tggaaagagt ttgctgaaca
      541 agaaggtgta aaatcacttg gaaaaataga tgaatcacca attccattag gatatttcaa
      601 agatactttt gaaaaagttc ttgaagtatc aagtgaagac gaaatattaa gtgaaataca
      661 aaaaataaga aatggttttg aagataagta tgacatagta gaaatgtttt attgtaaaga
      721 tggttgtaat aatggagatg gtttgtaaat ggtcaataaa tttaataata agtttttaag
      781 gctagtaaag agtgttctga tattttttat aattctttta ttatggaaaa ttacaaatta
      841 tcttggtatt tggagtgatt atatattacc atcacccgaa aaggtatata gtacattttt
      901 aaatatgata agtgatggtt caatatttat taatgtatat gcaagtatga aaagagtttt
      961 gataggtttt gcaataagta ctgctatagg tgtgccttta gggatattct ttggaattta
     1021 tagtggagta tacgaatact ttaaatctct aataaatttt ttaaggaata cacctcccct
     1081 tgctttaata ccaatgctta tattatggtt tggtatagga gaggaatcaa aaattataat
     1141 tatagtttta gcatcctttt tcccaatctt tacaagcaca ttgaaaggga ttaaaaattg
     1201 tgactcaaaa ctgattgaag tgggtagagt atttgagttt tcaaagcttc agataatatt
     1261 taaaataata ataccaaatg ccattcttga tattgctgtt gggttaaagt tagctcttgg
     1321 atatagcttt agagcaatta ttggggcaga acttgtagca gcttcatctg gtcttggata
     1381 cttgatatct gatggaaaag aaatgtctag aacagatgtt gttattgtag gtataatcgt
     1441 aattggactt ttaggaataa ttacagatta catattttcg attattgtga agaaggtaag
     1501 taagggaaag atggtggaag catatgaata atttagtatg tgactctgga tatataatta
     1561 gaaatttata taaaagttat aaagttgaca atgaagaaca tgtagttctt agtgatatct
     1621 ctttaaacat agatagaaac catataacag taattttagg tgaaagtggt tgtggaaaga
     1681 ctaccttgct tagagcaatt gcatcacttg aaggtataaa tagtggtgaa attaggttta
     1741 ttaatgataa taaagagtat agacctaatg ttggttttgt ttttcaggag agcagattaa
     1801 tgccttggtt aaacgtaagt gaaaatatag cttttcataa tcaaagaaaa aatactattt
     1861 taaacaaggt gcttagaaaa tttaaacaca ataaaagaat tagcagtaaa attataaatg
     1921 ataattttag aagcttagaa acaaatgata atttgaacaa atcaaataaa tataatagga
     1981 aaagtaatat tgatgtagaa aaatatctta agatgatgaa cttgcagaaa tttaaaaact
     2041 cttatccaaa tgaattgtca ggaggtatgg cacagagagt aagcatagct agagctcttt
     2101 ctttcaatcc agatatgttg cttatggacg agcctttttc ggcattagat tattttacaa
     2161 ggattgatat gcaaaatgaa gttataagaa tacataagtc aactaataaa ggcgtgatat
     2221 ttgtaactca tgatattgat gaagcgttaa agataggcaa gaaaatagtt gttttcacag
     2281 acgaaagaaa aataaaagaa tttaatatag aagatggtta taatagagat ttaacttcag
     2341 attactatat taaattaaaa aaagatattt taatgacaat gaaatcaaat aaaggcattt
     2401 aaagaaatta ataatatttt aaggagtaaa gtaaatgaaa ttaaaaaagc tattgatagg
     2461 tataacgata ttaacacttg caacagcatc tttagctgga tgtgctaaaa aaaataatgg
     2521 agaaaaactt tcagaaataa atttaactta tgtaaaatca ccattaaatg ttccttctat
     2581 aattcagaaa caggatgatt tatttggaaa ggaatttaaa aaggataata taaaagttaa
     2641 cttccacgaa attactacag gtccagagca aacacaagct ttagctgctg gagaaataga
     2701 cttcttacat gcactaggtg gtacatcagc tttaatagct gcatctaatg gagtagagtt
     2761 aaagatatta aacacttata gcaggtcacc aaaaggattt atgattttga ctaataataa
     2821 ttcaataaaa tcagcagcag atttagtagg taagaaggtg gcaggcccaa aagggactat
     2881 attacatcag gtattgattt cagcacttga taaagaagga ttaagtatgg atgatgtgga
     2941 atttgttaat atgggtattc cagaggcatc agcagctctt tctgatggca gcgtagatgc
     3001 tgcattgatt gctggtccag cagctcttaa agctatgaag tcaggtagta aacttgtagc
     3061 aaatggtgaa ggtttagtag atggtataat agtaactgct gttagcacag actttgcaga
     3121 gaaacatcca gaaatagttg aaagatttat gaaggttgaa aaggaaactt tagaatatgt
     3181 aaataataat tttgatgaag ctatggaaaa agtagctaaa gaagttgatt taagtcttga
     3241 agaaactaaa gaattatatg cttggtatga ttttagttta gatataacag ataaagacat
     3301 atcttcgctt gaagatacac aagacttctt aataaaaaat aagttacaag agaaaaaagt
     3361 gaacataaaa gaattaatct ataatgctaa gtagaaacta tgataaaata atactaagta
     3421 agaaatataa taaaagttta agttacctta ttaaggtaac tttttctatt ttgaattagt
     3481 aatttcaatt tattttagaa tgattagttg ataataatga ttaattaatc ataaaaagat
     3541 acttttttat agagttttat taagatgtct aataatatgg ttatacttat gaagtcagta
     3601 attgtgaagt tagtaatatg attaaattta tatattgcaa aaagattata atatattaaa
     3661 attggactgt tatatgatga gtaaataggt atattaggag gaattatatg agttataaag
     3721 tatatattgt ggaagatgat atatcaataa gtacattact taaagattat attgagaaat
     3781 atggatttga agtgattgtt gaagagaact ttgatgatat aatgattaca tttgaaagtt
     3841 ttaagccaca cttagttcta ttagatgtaa atttacctaa atttgatggt ttctactggt
     3901 gtagatgtat taggcaacag tcgaatttac ctataatttt tatatcagct agagatggta
     3961 atgtagacca aattagagct ttagaaagtg gagcagatga ttatataaca aaaccttttt
     4021 actatgatgt agtaatggca aaaataaaga gtcatataag aagggtatat ggtgaatatt
     4081 cacctaaaat agacgaaaga atagtggagc tagatggatt aaagttttat ccagaaaggt
     4141 tagaactaga attaaatggt gagaatctaa ttataactaa aaaagaaggt atactgattg
     4201 aatgtcttat caagagatac ccaaaagtag taagtagaga ttatctttta gaaaagatat
     4261 gggatgatat tgagtttgta gaggaaaata ctttaaatgt aaatgttagc aggataagga
     4321 aaagatttga ggaattaggt atagataatg ctgttcagac tgtccgaggg ttaggatata
     4381 aattaaataa gaattggtaa ggagaaagta tgaagttatt tttaaaagat tataaaggtt
     4441 atatttttat atattatata ggcatagtta tgacaattat ttactgtaat cttatgaagt
     4501 ttattagttt tggagagagt ttatatattc ttttatttag ttcttttata ttgttatgtt
     4561 ttttaatata taagtattat aaaaatagag aagtatataa tctgtttgaa aaaggagtac
     4621 attctttgga ggagtcattt ttgtatttag gaagctcatt cttggggaga aatatatcag
     4681 ttattttaaa acaacaacat aaattgtatc aatcaatgat acaagagcat aaaaagatac
     4741 atagtgaaca tttaactttc atcaaccaat ggattcatca gatgaaaaca ccaatatcag
     4801 ttataagcct tcagcttcaa gattatgagg gagaagaagt aactgaaaat ataagaaaag
     4861 aaatagatag attaaataaa gggttagata tggctatgca ttttgcaagg gcagataatt
     4921 ttcagaaaga ctttatagtt gaaaaagcca aattaaatga aattgtatct agtattgtaa
     4981 atgaagaaaa aaggttattt ataaaaagtg gaatgattcc taagattcaa gtagacaaat
     5041 ttacttatgt ttatacagat gtaaaatgga tgaaatttat aataggtcaa ttaattacaa
     5101 atggagtgaa atattctaaa gattatagta actacctaac tataaaatct gaagaaaata
     5161 ataaatatgt aattttaagt ataatagatg aaggaattgg aattgtttct aaagatttaa
     5221 aaagggtatt tgacccattt tttacaggag aaaatggtcg taagtttgga gaatctacag
     5281 gtatgggtct ttatctagta aaaaaggtat gtgatgattt aggtcataaa gtaagtataa
     5341 aatcagaagt aggaaaaggt acagaagttt caatctactt taataaagat gtaaatagtt
     5401 taataaaggt gtaaattatc tgttacaata gggtttttag ttaattgtac ttttttatta
     5461 tacaatttat taaatgaaat attcatagtt aaataatcaa aatttaagag agtttatttg
     5521 caaaccttac atagatgtaa ggtttgtgta atgtttgcag atagttaaat tttctaatct
     5581 aaattacaat taatataaga aataacataa gagaaataac ataaaaaaag aaattttgta
     5641 aaaaaggaag gatgtttatt gatgagttta gatgttattt ttatattgaa atcagtcata
     5701 atagctatag tagaggggct tacagaattt attccagttt catctacagg tcatatgatt
     5761 ttggttggta atttaataga ttttaagggg caatttgctg aaatgtttga ggttgtaata
     5821 caacttgggg ctatattagc agtagtagta ctttattgga aaaagataaa agatagcgtt
     5881 atagaatttt ttaaatttat atttactggc ggaaaagaag gaaagattgg ttttagattt
     5941 ggaatgaatg taattatagg ttgtatacca tttgcgatta taggagttct attttacgat
     6001 aatataaaat cattgtttaa tttacaatct gtaattatag gatttatagt gggtgggatt
     6061 ttattattag tagtagaaac tttgtttaga aaaaagaatc attccacaga taatatagat
     6121 aaaattactc caatacaagc tttaaaggtt gggacattac aagttttgtc tgcatggcca
     6181 ggtatgtcaa ggagtgcatc tactattatg ggtggatgga tagcaggact taattctcct
     6241 acagcagctg agttttcttt ctttttagca gtaccagcta tggtggcttc ttcaggaaaa
     6301 gatttatttg aatttgatta ttcaataatg acaccaacat tgtggattgc tttggtagtt
     6361 gggtttatag tagcttttat tgtatcaata atagttatgg aaaaattcgt gaatttttta
     6421 aagaaaaaac caatgagagt gtttgctgta tacagaataa tcatgggtgt agtacttgca
     6481 gtattagctt ttactaacat aattagtgta tagaaaaaat agatttatta tctataaaaa
     6541 ttaaactaag ataaattata attaacaatt atatatatgt tatttcaaaa attaaattag
     6601 gaaggatagt taaataatga aaattttaac tgtaaataat ttaagtaaag tatatggtaa
     6661 aaaaataatt ttcaacgcat taaatgacat aaattttagt atagaagatg gagaatttgt
     6721 aggaattatg ggtccatctg gtagtggaaa aactactctt ttaaatatga tttctactat
     6781 tgacaagcca actacaggaa ctatggaatt aaagggcaag aatcctttat tgttaagagg
     6841 tgaggaactt gcattattta gaagaagaga gttaggtttt gtatttcagg attttaattt
     6901 attggataca ttaactatag gagaaaatat agtattgcca ttgacattag ataaggtcag
     6961 tgtaaaagag caagatgaga gattgaatga agtatctaca atattaggta taaaagattt
     7021 attaggaaaa agaacttttg aagtatcagg tggtcaagct cagaggactg ctattgctag
     7081 agcattgatt aacaatccat caatactttt agcagatgaa ccaacaggta atttagattc
     7141 aaagtcatca aaggttgtaa tggagctatt tcaaaagata aataaagaaa ataaagttac
     7201 tactatgatg gtaactcatg acccattagc agcgagctac tgtagtagaa tcttattcat
     7261 aaaagatggg tctatatata atgaaatata taagggtagt agtagagagc agttttatca
     7321 agagataatg gatgtactta cattattggg aggcgataac taatgaatat aaaacagttt
     7381 gctattaaca atatttcaag aaatattaaa gcgtatttag gatacttagc aagtattgtt
     7441 atatcatctt cactgctatt ttcttttatt atgtttacaa gtcatccaga tttagatgta
     7501 tctcaatttc ctgattattt aaaagaaggt ctcaagatga gtaagataat tgcatatcta
     7561 ttcttattct tctttgtgtt ttattcagtt agtgtgtttc ttaaaagtag atataaagaa
     7621 tttggaatac tttatattac aggtatatca aaacgccaag taatgaaatt aatctttata
     7681 gaaaatatga taataaatat agtatcttct gtaattggaa taataatagg cttaatcttt
     7741 gcaaagatat ttttagtggt tatgtctact tttttagaat tatcaccatt aaaattctat
     7801 ataccattta atgcgatatt gtctacttta atctatttta tgattttagc tgtattaaca
     7861 tcaatattta cttcttttat agttaaagaa aatcaggtgt tgaagttact taaaggtaca
     7921 aaaactccaa aaccagagcc taaaacatca ctaatgcttg ctatattaag tatttgtttg
     7981 tttataatag cgtattataa tgctattacc tcaaccgacc aatatacaac atatctgcgc
     8041 ttagttccag ttacatccct tactatagta gcaacttact tgttcttttc tcaatttagt
     8101 gtatttttta tgaagaagct aaaattaaac aagagatttt atagaaaaaa tacaaatatg
     8161 ctttggattt cgaacttgat ttataaggta aaggataatg ctagaatatt tttcttgata
     8221 actataactt ctgcagtagc atttactgct ataggtacag tttattcctt ttggaaagat
     8281 gtagagcgtc aaattaattt aatatatcct aacacaatat attattcaac aatgacattg
     8341 cataatgacc ctaaaaagcc tgatagcaaa gaaaaagaca aggaaagaat gatgtttata
     8401 gaaaataatc taaaaaaaga gaatgtagac tatacaagga ctaatggtga atttaaaact
     8461 gtatttccca agaaaagtga ttttaatgtc agaattatga aagaatctaa gtacttagaa
     8521 attacagaaa ctataggggt taagcctata agttttgaag ataatgaatg tatatcatta
     8581 ttatcatcaa gtttaccagg aaataagaac atcaaagaaa atattattgt agggaataga
     8641 ggtttaaagg tggcaaaaca agtggaagaa tgtgttatgc cagcatatta caaaaatatg
     8701 tatgtggtaa aggataattt ttatgatagt atagaatcaa aatttatagt ggatagattt
     8761 actgcatttg aagtagagga ttcaagtaaa actatagaga tatgcagaaa atttgaaaat
     8821 aaatttaata atgagtcagg gatgcaacca tatttgtttt tctctaaaga attaatgata
     8881 ggaactggga aattaatgta ttctacattt atatttttag caatatttat aggtttaatt
     8941 ttctttgtta ctacaagtag ttttttatat aataaatttt atatggattg ccaagttgat
     9001 aagaaaaagt atgaacagtt aaataaattg ggtatgacat ataatgagat taaaaaagct
     9061 tcaacaattg agattggaat agtatttttg tttccttatg tagtggctgt tatacattca
     9121 atatttgcac ttactgcact taaaaattct tttgatatag aagtagcatc atcagctgtt
     9181 ttggtaatgg gaagcttgtt cttggttcaa atagtttatt ttttaattgt aagaaataat
     9241 tatttaaaag agataaggtt aaatttagtc aatttttagt tactattatt aaatg
//
LOCUS       DAGSRM010000124         9199 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.129, whole genome shotgun sequence.
ACCESSION   DAGSRM010000124 DAGSRM010000000
VERSION     DAGSRM010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9199
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.129"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(204..812)
                     /locus_tag="MJE33_003319"
     CDS             complement(204..812)
                     /locus_tag="MJE33_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBY9649235.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISD
                     FYYDDKGKKVFSKDELDIKTSKRINIDYAEEAKDFLWRFYIE"
     gene            complement(921..3659)
                     /locus_tag="MJE33_003320"
     CDS             complement(921..3659)
                     /locus_tag="MJE33_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HBY9649236.1"
                     /translation="MESKGTLSKNKVYILANGLFLVIALLNINLYTVSINMYRIILLW
                     IFGYSIVYISSFKTDEFINILKVGCLVLVFVSILGVVNLKIQKFQIVLLNLSIINMST
                     FIYLTISKVKKLRISPILIFYSAFVILDYFIRNIFSDINWNMIIILINICISMFNLLY
                     LLNKLDSNIEHYKLIKDLTYFVFFTTIICFVAISKINIVDSDRLIGVIYLLLCNHTYY
                     VYVYSLNNRVVYPYYELDSINKKLSKKSEQLGKINLAIEKDMIIQRTLKNYIDQRKEL
                     LRQALDTIPNVWIVTDYDFNISYTNNKFKDEFSKDMNNYYKILTFIKEDEKVAEKLKK
                     FDNDISIIDKLVELNDKIYLLSLSNNKDESNYLISLNDITNEIKIDEEIRNINKDYEN
                     IILNIPSPILVRSAEGGIKKNKVISINKKYEKSFKCVSSDLVDMTLEQYFETFNIDFF
                     DNREFKRLNLTQEKKAEIVSYNDTANCIVNFVMSDSEGEEHVEEVRVGDYWSDNKLFK
                     LLTFRDITKEINILRTVNEQKIIYEKLLDVIPEAIFLEDLSTSRVLYTNRAFRELFGI
                     SSDVLGVTTQKYRNILVKKYINNLNIGEREKSIHIVNENNHIKEVKMISRTLYFGQKR
                     SRVRIIKDLSVQRESERLKKALIKQRQYDQMKMEFYANISHELKTPLNNIYSSVQLIE
                     NLYKKGKIIDFQDILKEHIKITKQNMFRLLRLIDNIINISQVKSDIYKIKAVNFDIID
                     ITERIVTSISSYAKSKGIDLIFDTDEETVMVGLDPESIERIILNILSNAIKFTLEGGE
                     ILVGIYKKDETVEIIIKDTGVGIDKEKLKDIFNRFKQIENSGISNEFGSGIGLCLTKS
                     LIEIQNGKIYIDSKVGEGTNVKVIFPIKEVVEEVYDNSNYNDNIEKFEIEFFDIYK"
     gene            complement(3807..4598)
                     /locus_tag="MJE33_003321"
     CDS             complement(3807..4598)
                     /locus_tag="MJE33_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBY9649237.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKSLDELKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIVIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIQDEYKGSIVPAF"
     gene            complement(4634..5290)
                     /locus_tag="MJE33_003322"
     CDS             complement(4634..5290)
                     /locus_tag="MJE33_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9649238.1"
                     /translation="MNSLIDFLTTLFPNALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSTINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALIILVQIIQGVGNLAYKKLK
                     "
     gene            complement(5283..6248)
                     /locus_tag="MJE33_003323"
     CDS             complement(5283..6248)
                     /locus_tag="MJE33_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HBY9649239.1"
                     /translation="MISIKNVNKYYGKIQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGSVLVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERKTVFDNVALP
                     LECFGYSKAEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKKLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISDKSGEQVKQYLTSKGIRWEEMINE"
     regulatory      complement(6354..6460)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(6750..7694)
                     /locus_tag="MJE33_003324"
     CDS             complement(6750..7694)
                     /locus_tag="MJE33_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBY9649240.1"
                     /translation="MSRAERLIELMITINAKRSFTAGELAEEFSVSKRTILRDLQVLE
                     SIGFPLYSKVGAAGGYHVLKERILPPIAFSESEVKSIFFAYQSLEYYNDLPFEQETIS
                     VLKKFLNCIPNDIQYNIENIRRKFVFWTPDRHCSTPLLKELFNIVMNEFTIKIEYSSK
                     QKNSVRTIVPIGLYAMNGLWYCPAYCIKSESIKEFRVDRIVKILSIENLSSKKYKVLS
                     SIHDYLKNMEVGTDYHIKINLTDEGVKRCETEFLLARGLKILSKGGYIDMYIPKSTLN
                     WVAEYFLTFGKNATIIEPIELKHLIKSKVLELYNHHCI"
     gene            7805..8272
                     /locus_tag="MJE33_003325"
     CDS             7805..8272
                     /locus_tag="MJE33_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649241.1"
                     /translation="MENDTIKADDILNYCLSNLDDVVLMDSWGERAIYYNPNGVLKRG
                     VYVLTIKEKDSNNDKGSLVSRPNVYRVNIGLKKETFIEMFGYIPKRPGVGQIVDMDFD
                     FTKLDTIMPHPIYSWMGWICVLSPTEKTFENFKTLIGESYNFAKQKFKKRKNK"
     gene            complement(8442..9041)
                     /locus_tag="MJE33_003326"
     CDS             complement(8442..9041)
                     /locus_tag="MJE33_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649242.1"
                     /translation="MKLKRTLFVGLALVLAIGITACTKNNEKPSENTGKNPNESVNNN
                     EGYMNYYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRD
                     KIQSFVTSLKKDGKTEDTELKKMNDDLIAEGERLVKDIDKRIEKLDKVSDEDMKKGQN
                     DFIKLVHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
BASE COUNT         3008 a         1506 c          884 g         3801 t
ORIGIN      
        1 ataaaataca caaaaaaatt aatttttata gctcatttcg ccaaaatata tctttcttta
       61 tatcattaaa ataattataa atttattaaa tatgtttgta attctataaa gttattttta
      121 tcaaactaaa aattctataa aaaaataaga tacataccta taaaatacct tcctatagat
      181 acatatctta cctatataaa aacttattct atataaaaac gccataaaaa atcttttgct
      241 tcttctgcat aatctatatt tatacgttta ctagttttta tatctaactc atctttacta
      301 aatacttttt ttcctttatc atcataataa aaatcactta tatataattc ttcacccatt
      361 atactcttac tatttagact tctatctatt tttaaagctt tgcaaagttt tcctggtccg
      421 ttagtaatat tttttacttg atatttactc aactcagtgt aagctttgtt gtatctattc
      481 atagatatct catctaaaga agttataggc tctacccctc ttattaatac acactctggc
      541 acattttcta tatttgcaga taaattcaag caattataca taccataaat taaataaaca
      601 tatatatgtc caccatctaa atataatggc ttagttctat ctgtcttttt attgccatat
      661 acatgtgctc ctttatcatt tacacccatg tatgcttctg tctctatgat tttggtaaca
      721 attactttat tttcatattt ccttattaga tatttaccta atattgattt ggctaaatca
      781 attccatttt ttctaaaaaa atccttttcc aaaatttcac ctcataaaat ttatttaaaa
      841 cgcatcaaat acaactaaag taataaataa aattaactct tattaatgtg atatatataa
      901 acaataaatt tagacattta ttatttatat atatcaaaaa attctatctc aaacttttca
      961 atattatcat tgtaatttga attatcatat acttcttcaa caacctcttt aattgggaat
     1021 ataactttta cattagttcc ttctcctacc ttactatcta tgtaaatctt cccattttga
     1081 atttcaatca atgattttgt aaggcataaa cctataccac ttccaaattc atttgatata
     1141 ccactgtttt caatttgttt aaatcgatta aatatatcct ttaatttttc tttgtctatg
     1201 cccactccag tatcttttat aattatctct actgtttcgt cttttttata tatacctaca
     1261 aggatttctc ctccttctaa agtaaatttt atagcatttg ataatatatt tagtattatt
     1321 ctttctatcg attctgggtc aagccctacc ataacagttt cttcatctgt atcaaaaatt
     1381 aaatctattc ctttagactt agcataacta ctaatagatg taactattct ttctgttatg
     1441 tcaataatat caaaattaac tgctttaatc ttatatatgt ctgacttaac ttgagaaata
     1501 tttattatat tatctattaa tctcaataat ctaaacatat tttgtttagt tatttttata
     1561 tgttctttta gtatatcctg aaaatcaatt atttttcctt ttttataaag gttttcaata
     1621 agttgaactg aactatatat attatttaaa ggtgtcttaa gttcatgaga tatattagca
     1681 taaaactcca tcttcatttg gtcatactgt ctttgtttta ttaatgcttt tttaagtctt
     1741 tcagactctc tttgcacact taaatcttta ataattctca ctctacttct tttctgtcca
     1801 aagtataatg ttcttgatat catttttact tctttaatgt gattattttc atttactata
     1861 tggatactct tctctctttc tcctatattt aaattgttta tgtatttttt tactaatata
     1921 tttctatatt tttgtgttgt aactcctaat acatcactag aaattccgaa caactctcta
     1981 aaggctctat ttgtatatag tactcttgat gtacttaaat cctcaagaaa aatagcctct
     2041 ggtataacat ccagtaattt ttcatatatt attttttgtt catttactgt tctaagaata
     2101 tttatctctt tagttatatc tctaaatgta agcaatttaa agagtttatt atctgaccaa
     2161 tagtctccaa ctctaacttc ttcaacatgt tcttcacctt ctgagtcaga catcacaaaa
     2221 ttaactatac agtttgctgt gtcattgtaa cttactattt cagctttctt ttcttgtgtt
     2281 agatttaatc ttttaaattc cctattgtca aagaaatcta tgttaaaagt ttcaaaatat
     2341 tgttctaatg tcatatcgac taaatcactt gaaacacatt taaaactctt ttcatatttt
     2401 ttatttatac ttattacctt attcttcttt atgccacctt cagcacttct tacaagtata
     2461 ggagatggaa tatttagtat aatgttttca tagtctttat ttatatttct tatttcttca
     2521 tctattttga tttcatttgt aatatcattt aaacttatta agtaattgct ttcgtcttta
     2581 ttattactta aacttaacaa ataaatttta tcatttaact ctaccagttt atcaattata
     2641 cttatatcgt tatcaaattt cttcaatttt tcagcaactt tttcatcttc ctttataaaa
     2701 gttaatattt tataataatt attcatatcc tttgaaaatt catctttaaa tttattattt
     2761 gtgtaactta tattaaaatc atagtctgtt actatccata catttggtat tgtatccaga
     2821 gcttgtctaa gtaattcttt tctctggtct atgtagtttt ttaatgttct ttgtataatc
     2881 atatcctttt ctattgctaa atttatcttt cctaattgct ctgacttttt acttaatttt
     2941 ttattgattg aatcaagttc ataatatgga tacactactc tattattaag actgtataca
     3001 tatacataat aagtatggtt acataatagt aaatatatta ctcctattaa tctatctgaa
     3061 tctactatat taatcttaga aattgctaca aaacatataa tagttgtaaa aaaaacaaaa
     3121 tatgttaaat cttttattaa cttatagtgc tcaatgttac tatcaagttt attcaacaaa
     3181 tatagtaaat taaacataga tatacaaata ttaattaata taataatcat attccagtta
     3241 atatcactaa aaatatttct tataaagtaa tctaatatca caaaagcact ataaaaaatt
     3301 agtattgggc ttattcttag tttttttact ttagaaatgg ttaagtatat aaaagtagac
     3361 atattaatta tgcttaagtt aagcaataca atttggaatt tttgaatttt taaatttact
     3421 actcctaaaa tacttacaaa aactaataca agacacccta ctttcaatat atttataaat
     3481 tcatctgttt taaagctact tatataaact atagaatatc caaatatcca aagtaaaatt
     3541 attctataca tatttataga aactgtatac aaatttatat ttaaaagtgc aataaccaaa
     3601 aataacccat tagctagtat atagacctta ttcttactta aagttccttt agattccata
     3661 ttccccacca cttcttcatt tttatatagt tttacctata aatatgttac cacaattctt
     3721 ccaaaaaatt tgtcactttc tagttataaa aaaaaatgag taaatatttt taattttact
     3781 cattttgtag attttgtata ttcagtttaa aaagctggaa ctatacttcc tttatattca
     3841 tcttgtataa attttttaac ttctttacta ttcatagcct ctgataaagc cttaatctta
     3901 tcactatctt tattattttc tctacaagct attatattta catatggaga atcacttgat
     3961 tctattacta ttgcatcctt agtaggattt aagttagctg ttaaagcata attagaattt
     4021 attactgctc cgtcaacatc ttttaatact gttggtaatt gctctgcatt catttctttt
     4081 attactatat ttttaggatt tttagttata tcttttttag ttataagctc tccatctttt
     4141 acttctatca atttattttt tgctaataat ttaagtgctc tagctccatt agttgcatca
     4201 tttggcacag ctataacagc tccatctttt aattcatcta atgacttaac tttttctgaa
     4261 tagaatccca ttggctctat atgaacttta gatgtataag taagtttata tcctttctcc
     4321 ttcacagttt cctctaaaaa tggaatatgt tgaaagaagt tagcatccaa agacccttca
     4381 tctaaagctg tatttggaag tacataatca tcaaatattt ttacttctaa atcatatcct
     4441 ttttctttta acaaaggttt tgcaacttcc aatatctttg catgtggatt tgatgaagct
     4501 cctactagta tttttttatc ctctttgttt gaacaaccta ctgctgatat tgctattgct
     4561 gacaccaatg ctactgataa taatttcttt aatttcattt ttactccccc tctttaaaat
     4621 gtttataatt aatttatttt agtttcttat aagccagatt tcctactccc tggattatct
     4681 gaactaatat tataagtgct attacagtat ataccattac tgctgtatca aatctttgat
     4741 atccataaat aagtgctata ttacctaatc ctccagctcc tactgcacct gccatagctg
     4801 tataaccaag tatagatatt attgataatg taattccaga tactattgaa ggcatagctt
     4861 cctttatcat aactttaaat actatttgtc tttttgtagc tccaaaagat tttgctgctt
     4921 ctatcaatcc tttatcaact tcatttaatg agctttctat aattctagcg ataaacggtg
     4981 ctgccccaat agttattggc actattgcag cagtttcccc tatacttgtt ccaacgatta
     5041 atctagttat tggaatcatt gcaactataa gtatcataaa tgggaaactt ctaaatatat
     5101 ttactataaa tcctaatgca ctatttattg tgctatttgg ttttaatcca tctggttttg
     5161 taactactaa tatgattgct aatataaatc ctaatattgt tgccactata gttggtacta
     5221 taaccatata caaagtttgt aacaatgcat ttggaaatag tgttgttaaa aagtcaatta
     5281 aactattcat ttatcatttc ctcccatctt atgcccttac tagttagata ttgttttact
     5341 tgctctccac ttttatctga aatgtttata ataagtgaac ctaaaatatc atctttaaat
     5401 tgttctaatt tcccaaatat tatagacaca tctatattta gttctcttgc catagtcgta
     5461 ataatagctt cattagaaat atctttaggg aatagtatct ttatatttgt tccttttggc
     5521 aatattatac tttcttcacc tattaatttt ctaagtcctt tagtatttct taagaatatt
     5581 tcttctgtat ctcctacctc taatacttct ccattttcca ttattgctac tcttccacaa
     5641 atctgcttta taacttccat ttgatgagtt acaactatta ttgttattcc caatttttta
     5701 tttatatctt caagcaatga aagaattgac tttgtagtat ttgggtctaa tgctgaagtc
     5761 gcttcatcac aaagaagtac ttgtggattt agtgctaaag ctctagcaat tgcaaccctt
     5821 tgtttttgtc cgccacttag atttctgggt ttatcatttt tcttttcaga tattccaact
     5881 acttctaata actctaaaac tcttttttta atttctgcct ttgagtatcc aaaacattct
     5941 aatggcaatg ctacattatc aaatacagtt tttctttcaa gcaatgaaaa gtgttgaaaa
     6001 atcattccta actcttttct taaatctctc atttgctttt cattcaatga ctttacttct
     6061 ttatcagaaa caagtacact gccttcttgg tattcttcta atccgtttat acatctcagt
     6121 agtgttgatt tccctgcacc actatgacct attattccaa aaatttctcc agattctatt
     6181 tcaatactta catctttaag cacttgaatc tttccataat acttattaac attttttatg
     6241 ctaatcataa aatttcctcc catatattct gctcagtttt tatgtttatc ctctcttatg
     6301 tattacaaca aaataaaagc ccgagtataa actcaggctt agaaagctat ttatactcat
     6361 ctatcaacta tattagttgc aggatttagc accaatgtat ataaaatata ctggttgccg
     6421 ggtttcaaag ggccagtccc tccaccactc ttgataagac gttatttaat ttaattctat
     6481 tatcatttgt tgctatgtta tatatgatat attcattaat agtatttgtc aataaattta
     6541 tttaaatttt ttatttttta ttatcgttaa ttaactattt ttaattagtt ttctagctcc
     6601 taacacttta tattttaatg tctatataac tatagaacat cattataaga aataaattaa
     6661 ataacatcaa tctatctaaa tcatctgtaa agtaaattaa gaaaattaac attttatcca
     6721 aactctatcc tactattaaa taaaataatt taaatacaat gatgattata tagctctagt
     6781 actttagact ttatcaaatg tttaagttct ataggttcta ttattgtagc atttttccca
     6841 aaagttaaaa aatattcagc cacccagtta agtgtagatt ttggtatata catatctatg
     6901 tatccacctt tagacaagat tttcaaccct cttgccaaca aaaattctgt ttcacaacgt
     6961 tttactccct catctgtcaa attaatttta atatgataat ctgtacctac ttccatattc
     7021 ttaagataat catgtataga agataaaacc ttatatttct tacttgataa gttttctata
     7081 ctcaaaatct tcacaattct gtctacacga aattccttaa tagattctga ttttatacag
     7141 taagctggac aataccaaag tccattcatg gcatacaaac caataggaac aatggtacgt
     7201 acactatttt tttgctttga tgaatattca attttaattg taaattcatt cattactata
     7261 ttaaacaatt ctttcaataa tggtgttgaa cagtgcctat ctggtgtcca aaaaacaaat
     7321 ttacgcctta tattctcaat attatattga atatcatttg gtatacaatt taaaaatttt
     7381 tttaaaacag aaatagtttc ttgctcaaat ggcaaatcat tataatattc tagtgactga
     7441 tatgcaaaga atatagattt tacctcactt tctgaaaatg caatcggtgg aagtatacgc
     7501 tctttaagta catgatatcc accagccgct ccaacttttg aatataatgg aaatccaata
     7561 ctctccagca cctgtaaatc tcttagtata gtacgttttg atacagaaaa ttcttctgct
     7621 aattctcctg ctgtaaaact tctttttgca tttattgtta tcatcagttc aattaaacgc
     7681 tcagctcttg acatatttat actcctaatt taaatatagt gacaataatt gtcactatta
     7741 tatgataaga tattcttgca tctaaagcaa taataatagt aaagaaagaa gggattgata
     7801 ctttatggaa aatgatacta ttaaggctga tgatattctc aattattgtc tatcaaactt
     7861 agatgatgtt gtactaatgg atagttgggg ggaacgagca atttactaca atcctaatgg
     7921 tgttttaaag cgaggggtat atgttcttac cattaaggaa aaagacagta ataatgataa
     7981 aggttcgtta gttagtcgtc caaatgtata ccgtgtgaat ataggattaa aaaaagaaac
     8041 ttttattgaa atgtttggat atattccaaa gcgtccaggt gtaggtcaaa tagttgatat
     8101 ggattttgat tttacaaaat tggacacaat catgccacat cctatctact catggatggg
     8161 atggatatgt gtcctaagcc ctactgaaaa gacatttgag aactttaaaa ctttaatagg
     8221 agaatcttac aattttgcaa aacaaaaatt taaaaagaga aaaaacaagt aaaaacgtgt
     8281 gaaaataacc tctaaataaa ttataataaa aatattaagg ttacaagata aaaattaata
     8341 tcttaataac atgcaaaaaa gcgaagccaa aaaatgtatt atttataagg cttcgctata
     8401 tctcaaataa tttatttcaa atttcaaaac tatttattct attatttctt attatttcct
     8461 gttattccta atctatcatt catatctttt atcatttttc taaaaccact acctatatca
     8521 tttccaacat cttcagttcc atgaactaat tttataaagt cattttgacc ttttttcata
     8581 tcttcatcag ataccttatc taatttttct attctcttat ctatatcttt tactaatctt
     8641 tccccttcag cgattaggtc atcattcatt ttctttaact ctgtatcttc tgttttacca
     8701 tcttttttaa gagatgttac aaatgattgt attttatctc tactatcttt atatgcagcc
     8761 tttacatcac ttagatattt ttcatttcct ggatattctt tatctttaaa ctgattattt
     8821 atactttctg gagtatcata aatagaatat ctatttagac ctgctatata atcattatag
     8881 cttgttgaat aataattcat atatccttca ttattattta cagattcatt aggatttttt
     8941 cctgtatttt cacttggttt ttcattattt ttagtacatg cagttatacc tatagctaat
     9001 actagtgcta atcctacaaa taaagttctt tttaatttca tttttattac cctcctagat
     9061 gttttatact ataaaaaatt ttaataattt tctttcttaa tttagtattt ctatattttt
     9121 atattttatt tactaaaatt aaacaagtta taatttattt ttttggaaat cataatgaat
     9181 aaataattaa ataaaaaaa
//
LOCUS       DAGSRM010000125         9039 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.130, whole genome shotgun sequence.
ACCESSION   DAGSRM010000125 DAGSRM010000000
VERSION     DAGSRM010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9039
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.130"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            240..1445
                     /locus_tag="MJE33_003327"
     CDS             240..1445
                     /locus_tag="MJE33_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBY9649243.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTEHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVEPSKIRTFGIPVEKSFLSHRDKG
                     IVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTDRDFQILVITGKNESLKEKIE
                     KKLMSRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNIKSIRKSNSAQNIA
                     NLILNIISG"
     gene            complement(1516..1737)
                     /locus_tag="MJE33_003328"
     CDS             complement(1516..1737)
                     /locus_tag="MJE33_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649244.1"
                     /translation="MKPIMNLEEHNAMKEKRVKKMKSKDKPKYDDFEDISFSNKKKTK
                     KNVRNKNAEFRNNYKNYQYDYEEDFYEPI"
     gene            complement(1878..2477)
                     /gene="recR"
                     /locus_tag="MJE33_003329"
     CDS             complement(1878..2477)
                     /gene="recR"
                     /locus_tag="MJE33_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBY9649245.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKREIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            complement(2490..2840)
                     /locus_tag="MJE33_003330"
     CDS             complement(2490..2840)
                     /locus_tag="MJE33_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBY9649246.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            complement(2899..4536)
                     /gene="dnaX"
                     /locus_tag="MJE33_003331"
     CDS             complement(2899..4536)
                     /gene="dnaX"
                     /locus_tag="MJE33_003331"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBY9649247.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKEYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKDSIDENESK"
     gene            complement(4613..4877)
                     /gene="ffs"
                     /locus_tag="MJE33_003332"
     ncRNA           complement(4613..4877)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="MJE33_003332"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(4915..5006)
                     /locus_tag="MJE33_003333"
     tRNA            complement(4915..5006)
                     /locus_tag="MJE33_003333"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4970..4972),aa:Ser,seq:gga)
     gene            complement(5007..5462)
                     /locus_tag="MJE33_003334"
     CDS             complement(5007..5462)
                     /locus_tag="MJE33_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HBY9649248.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
     gene            complement(5783..7054)
                     /gene="serS"
                     /locus_tag="MJE33_003335"
     CDS             complement(5783..7054)
                     /gene="serS"
                     /locus_tag="MJE33_003335"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBY9649249.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEREFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKKIPQLIKEGKDVTEEKARLKELSDKIKGIDEKVKEVEAKMEYTLM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEHKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIK"
     misc_binding    complement(7180..7385)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(7513..8394)
                     /locus_tag="MJE33_003336"
     CDS             complement(7513..8394)
                     /locus_tag="MJE33_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBY9649250.1"
                     /translation="MNFLSLAKTASTNSNITKRTLENMLDNFSDNIPNIIYAIIVFVI
                     GIYISKIVRRMVSKFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGIIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDVVKSILNEIFTNEKR
                     ILNDPKPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENDIKVPFVP
                     SSLLFNNTGNLNTMDKR"
     gene            complement(8406..8915)
                     /locus_tag="MJE33_003337"
     CDS             complement(8406..8915)
                     /locus_tag="MJE33_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBY9649251.1"
                     /translation="MNSNERRSKLIDILKESKHPVKGGTLAELLNVSRQVIVQDIALI
                     RARGFEIIATPQGYIIYNQPYFTKQIKCKNHKDSKEIYDELKIIVDLGGIIKDVIVNH
                     PTYGQITAELNIYSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIEAIKEETIEAIVK
                     ELKDNGILS"
BASE COUNT         2897 a         1580 c          965 g         3597 t
ORIGIN      
        1 aatatatatt ctatgctcta ttttcctttt tattctatat ttaaattttt tatttacaag
       61 aatattttta atataacata tttgataaaa ttaatttaca tttctttcac ttgtaatttt
      121 tgcaatcttg tattagatta atattaaaaa taatttatca ataaccttat tcacaaagac
      181 agtttttata tatcaaaata taatataaat actatgctac attttaggag gattttatta
      241 tgagcaaaaa agtattaata atgtctgctt ccactggtgg tgggcataat agggctgctc
      301 tagctattaa agaagaactt acttccaaaa cacttgatgg agagccaatt gaatgtgaaa
      361 taatagatag tttgaaatta gttaataata caatggataa aataatttca agaggatacg
      421 aaaaatctgc tatatatact cccaaagcct atggaagtgt atatagatta tctgaaacaa
      481 atcttttatc aaaaaatgaa tttaaagata atctactaat aacattcatg gctaaaaaat
      541 ttaaaaaact tattagaagc gaaaaaccag acttgattat aggaacgcat ccttttccaa
      601 tgattgctct aagtacattg aaaaaaaact ttaatttaca taataatgaa tcaaatgctt
      661 acacagagca tttttataaa cattatacaa ataccattaa tgttcctccg cttatttctg
      721 tattaaccga ttatactact cattctacat ggatacaaaa tgaaattgat tactatattg
      781 ttggtcatga atatgtaaaa gaattattgg tgtttgatgg agttgaaccg agtaaaatta
      841 gaacatttgg aatccctgtt gaaaaatctt ttctctctca cagagataaa ggtattgttc
      901 tttctgagtt gaatttatct cctgataaat taactgtttt acttatgggg ggtagctttg
      961 gggctggaaa tataaaagag acacttgatg aattactgga tactgataga gactttcaaa
     1021 tacttgtaat tactggtaag aatgaaagcc taaaagaaaa aatcgaaaaa aaacttatgt
     1081 ctcgttatca tgataaaaat gtatgtgttc ttggatacac tgataagatg aatgatattt
     1141 tagcttctat agatgtatta gtaagcaaac ctggtggact tactacaact gaggctcttt
     1201 taaaagatgt acctatgata gttccttatt atataccagg tcaggaagaa gagaatcttg
     1261 atttcttatc taattgtggt gcagcactta gaactacaaa aaaatataac ttgtcaatat
     1321 tactaaaagt tttaatagat gaccctagca gattagaaat gctaaaaaga aatattaaat
     1381 caataagaaa gtcaaattca gcccaaaata tagctaattt aattttaaat ataatttctg
     1441 gttgataaaa taaataaaat ccctatagta tatatattat ctattaggga ttttttattt
     1501 tctaattatt taatattaaa ttggttcata aaagtcttct tcataatcat actgataatt
     1561 tttataatta tttctaaatt cagcattttt atttcgtaca ttttttttag tttttttctt
     1621 attagaaaaa cttatatcct caaaatcatc atatttaggt ttatcttttg atttcatttt
     1681 tttaactctt ttttctttca ttgcattatg ttcttctaag ttcataattg gtttcatgtt
     1741 gcacccctat caaaattttt aaatgattta tacaacaaat tatacaatac agaagaaatt
     1801 tattcaataa atctaaatca aaattaattt gaatttaact caaaaaatta caaaaatcga
     1861 ggtattaacc tcgattttta tatttctctt ctgccttcta gtgcttttga tattgtgacc
     1921 tcatctgcat actctaaatc gcctccaaca ggaagaccat gagctatccg agtgactttg
     1981 attcccattg gttttactaa cctagcaata tacatggctg tagcttcacc ttcaatagta
     2041 ggatttgttg ccattattat ttctttgact tcctgattgc caagtctttg gataagttcc
     2101 tttattttta acatatctgg tccaattcca tccattggag aaattactcc atttaataca
     2161 tggtactgtc ctttaaactc tctcgttctc tccatagctg ctacatctct tggatcttcc
     2221 acaacacata taacacttga atctctactt ttatttgaac acatgctaca tgggtctgta
     2281 tctgttatat tacaacaaat tgagcaatat cttatttctc tttttgcatc tataatcgct
     2341 ttagataaag cttccacatc attagtattc atattaataa tatgaaaagc caatctttga
     2401 gcagtctttc ttcctactcc aggaagcttt gaaaattcct ctataagtct cgttatggga
     2461 cctgtataaa cttgcataaa taccacctac tagaataacc caggtatatt catacctcca
     2521 gttaccttac tcatttgact tgcttgtata tcatcaatat ttctcaatgc ttgatttact
     2581 gcacttaaaa ctaaatcttg tagcatttct atatcatctg gatcaactac ttctggcttg
     2641 atagttatat ctataacttc ttttttacca ttaactttta cagtaactgc tcctccacca
     2701 actgaagctt ctacttcttt actttctaac tcttgttgag ctttttgcat attttcttgc
     2761 attttttgag cctgctttaa caagttgttc atatttccac caccaggcat cattccgcct
     2821 ccaaaacctt ttttagccat aaacactatt cctcctaaac ttattttcat tttattatta
     2881 tatcataata gtattttatt atttactctc attttcatca atactatctt ttatttctaa
     2941 tatctcttca ctcactatgt tctttaatat ttcctcacca atgtcctttt tctctttaac
     3001 ttcaagattt atatctttaa cttctgatgt cagtactatt tttacattaa aacttctatt
     3061 taaaacatct cttattgttt tttctaaata atttattgta tcttgagagc tcagtctagt
     3121 ttttgcaaaa tcaaatttat catcaaatat aacatacaac atattagaat atacattaaa
     3181 actttttact tcacttaaga gtgcgtatat tggcattttt ttatcttttt taatttgttt
     3241 aagtatattc ttccaagatg actctataag tataacgtcc tcatttttta catcttcata
     3301 aactacattt tcttgtttta catctatttc tctatcgacc tcatactctt tttctttatt
     3361 attcccaatt ttttcagact taaagttccc cagttcaatc attttttcta agttctcaac
     3421 tctttttata agagcttctt tactttcatc aaacattggt tgagcaattt tcattatggt
     3481 tatttccact aaaacccttg gatttgaaga tgactttata tcatcctgag ttattgataa
     3541 tatatttaaa atccttatta aatcatttat atctatattt ttagactgaa ttttaagttg
     3601 ttctatagtt tcttctggta atgaaattat ttcatccaat tctgatgaaa ccttacatac
     3661 cattaaattt ctaaaatgat caattaagtc atttataaga tttcttatat ctttgcccca
     3721 taagacaaat tcatttaata tctcaagaga ctttttagtg tcctgctcta ctatacattg
     3781 agacaattca aataattgtt ctatatttac gcttcccatt aattcaacaa catctttata
     3841 atctatctta ctttctccaa aagacataca ttggtctaat atactaagtg catctctaag
     3901 agctccttga gaatttctag ctattaaatt aagagcttta tcatctatat caattccttc
     3961 atcttcacaa atctttttca ttctaagggt catatcttta actgtaactc ttttaaaatc
     4021 gtatctttgg catcttgata aaatggttgc aggtattttg tgaggctctg tagttgctaa
     4081 tataaaaatt acatatgatg gaggttcctc taaggtcttt aataatgcat taaaagctcc
     4141 ttgagacaac atatgaactt catctattat atatacttta tatttagcat tggctggaga
     4201 atatttcaca ctttctctaa gctctcttat atcatctaca ctattatttg aagctgcatc
     4261 tatttctaca acatccattg tattatcttt taatacagat tcgcatatct cacactcatt
     4321 acacggttct tcatttattt tattcaaaca gtttactgct cttgaaaaaa tctttgcagt
     4381 agatgtttta ccagtacctc ttgttccaca aaacaaataa gcatgtccta tattatcact
     4441 atatatttga ttctttaaag tcttaataat atgatcttga ccaataacat cttcaaattt
     4501 ttgtggtcta tatgctctat ataatgcttt atgcatcttt ttacctctct ttagcaaaat
     4561 taataaaaaa atccctcatt atctaaggga ttagtctttt atatattaaa tgctgtacac
     4621 catctatcga cttaattcca taggcgttac caaaacagtt agctcaactt aggcaccccc
     4681 acggcacaca aaagtgatca cttaccgctg cttccttccg gatctgacgg ggttcatgag
     4741 ctttcattgc gcaggaccta agcatcaaca ccacttattt agggcggccc tacagaaaat
     4801 aagcctttag atggaattca atcctgctat agcggattgc aggttacagg gcaccgctac
     4861 ctccccatct agtacagcaa aattacaacc atatttaatt actttcattg tttctggcgg
     4921 agagagtggg atttgaaccc acgatacagt tttacccata tactcgcttt ccaggcgagc
     4981 gccttcgacc actcgaccat ctctccttaa tctctttttc tcaatgcctt aaaaaattct
     5041 tgcaatatga tcgagcattc ttcttgcaaa acatcaaaag ctacttttac attattatta
     5101 ttaaaataat ctaatttaaa ctcatgctgt ttttctatat atgaattttt tacatgtctt
     5161 gtccctatta taagcttttt tattctagaa ttaactattg cgccactaca cattatgcaa
     5221 ggttccattg taacatataa atcacaatca gtcaacctcc aaccacctaa tttttctgat
     5281 gcttgtttta tcgctaaaat ttctgcatgt gccgtagagt cttttaacgt ttctgtaagg
     5341 ttatgagctc tagctattat ttgattatct ttaactataa tagcacctat tggtgtttct
     5401 tttttattat atgctttata tgcttctttt aatgcttctt tcatataaaa tgaactttcc
     5461 ataattcctc ccaaaactta taacaaactt atcttatcat aattcatttt ataattcaac
     5521 ttaattttat tttgtttatt taattattac tatatatagc tatatttttt taaaattagt
     5581 tctaaaataa tttttagata ttcatacaag agtgttttat aaaaatattt agtgttttat
     5641 gatgaaaatt ctgctgtgta aattaacata aaaaaactat gaaattaata tttataataa
     5701 tttcatagtt tttttattta ttgtttactc ataatatatt ctcttcatca aagcatactc
     5761 tatatataca attaatataa atttatttta taacatcaac acccatataa ggtcttaatt
     5821 cttctggaac tactacagaa ccatctgcct gctgataatt ttctaatata gcagccaaac
     5881 atcttcctat tgctaatcca gaaccattta atgtatgaac atattctgct tttgattttt
     5941 tatctctttt aaatcttatt cctgctcttc ttgcttggaa atcttcaaaa tttgaacaag
     6001 aagatatttc tacatatcta ttgtaacttg gcatccaaac ttctaaatca tatttaaatg
     6061 cagctgtaaa tcccaaatct cctgtacata tttttacaac tctatatgga agacctaaca
     6121 tttgtaacat agtttcagca tcatgagtta atgattctaa ttcattataa gaatcttctg
     6181 gtttaacaaa tttaactaat tcaactttat taaattgatg ttgtctaact aaacctcttg
     6241 tatctcttcc tgctgaacct gcttcagatc taaaacaagg agtatatgca cagtatttaa
     6301 ttggaagctc tgcaacatct aatatttcat tagcatgtat attagtaaca ggaacttcag
     6361 ctgttggtat taagaaatac tctaatcctt ctattttaaa catatcttct tcaaatttag
     6421 gaagttgacc agtccctata aaactatttc tattggccat aaatggaggt aagacttccg
     6481 tatagccatg cttagctgta tgagtattta aaaagaaatt cattaaagat ctttctaatc
     6541 tagctcctaa tcccctatat aaagtaaacc ttgacccagt tattttccct gctctttcaa
     6601 aatctaaaat acctagacct gtacctatat cccaatgcgc tttatgctca aaatcaaatt
     6661 tagtaggttc tccccaagtt cttatttgaa cattgtcttc atctgtttca ccttgtggta
     6721 cctctggatg aggaacattt ggtattctca ttaatgtata ttccattttt gcttctactt
     6781 cttttacttt ctcatcaata ccttttatct tatctgataa ctctttcaat cttgcctttt
     6841 cttctgttac atcttttcct tcttttatta attgaggtat ttttttagaa tttacattta
     6901 actcattttt cataacttca acttcttgaa gtatttctct tcttttatta tctaattcca
     6961 ctacagcatc aaggtcaaat tctctttcac ctcttctttc catagctttt ttgatatcat
     7021 ctagattttc tcttattctt ttaatatcta acatattaat tcctcctaat aattttgata
     7081 atataagtta taattccaac ttaatttaca aacattatac ttgtaaactc ttagtttaaa
     7141 caaattttaa aaataaaaaa agacctccgt ttaccctata aaagggacga aaatctttta
     7201 tccgcgttgc caccctaatt gactatacta aaatgtataa tcctctcaga cctataacgt
     7261 aggtaaacgt ctagctttaa ctagaagctc tagagtagat tcaaaaacca ttgtaatgag
     7321 ttttcaccat ccactcattc tctgaataca atctgttttt tactattttc cttcacagct
     7381 ttttaactaa ttgttttatt tgataatcat tatattatat atattaacat ctttcaatac
     7441 tattattcaa atttttttag aattttcttt aattttttat attaaattca ttttttctat
     7501 ttaattaaaa ccttatcttt tatccattgt atttagatta cctgtattat taaataataa
     7561 tgaagaagga acaaatggaa cttttatatc attctcatca aatttatttt ttaccttttc
     7621 cattacatca aaatatacat cccagtaatc atcagttttt acccaaggtc tagcaactat
     7681 ttgcatggtg ttatttccta tagagtttaa acctattata ggctttggat catttaatat
     7741 tcttttttcg tttgtaaaaa tttcatttaa tatagactta actacatcta tatcactatc
     7801 atatgaaata ttatatgaaa aatctattct tcttttgttt tgatgtgtgt aattaattat
     7861 attattactt gtcagttgaa aattaggtat aattatagtt ttattatctg gcgtcttaag
     7921 aacagtgcta aatatttgta tgtcagaaac tgtaccttct acacctgaac cttgaatata
     7981 atcacctata ttaaatggct taaaaaataa aataatcata ccagagccta aatttgataa
     8041 tatttcttta aaagctaaac ctatactaaa tccagcagct cctaacacag ctacaactga
     8101 tgtagtttgt atccctataa tacctaagct tatcaatgat ataatagata acatagatat
     8161 atatattcca tatacaataa aattatttac acctttactc attccatatt tgattagaaa
     8221 ctttgaaacc atccttctta ctattttaga tatataaata cctatcacaa atacaattat
     8281 tgcatatata atatttggta tattatcact aaaattatcg agcatatttt ctaaagttct
     8341 cttagttata ttgctatttg ttgaggcagt cttagccaat gataaaaaat tcattcaact
     8401 cctccttatg ataatattcc gttgtccttt aactctttta ctatagcttc tatagtttct
     8461 tcttttattg cctcaattgt atgaatatgg ctacattctg tcagaaccga taattgttta
     8521 aattcatttg tctcaacttt cttcataaag ttgtctatat ccattttaga ataaatatta
     8581 agttctgcag ttatctgacc atatgttgga tgatttacga tgacatcttt tataatacca
     8641 cctaaatcca caattatctt taattcatca taaatttctt tagaatcctt atgattttta
     8701 cactttattt gttttgtaaa atacggttga ttatatatta tatatccttg aggtgtagct
     8761 attatttcaa atcctctagc tcttataagg gctatatctt gaactattac ttgcctactt
     8821 acatttaata gttcagctag tgttccacct tttacaggat gcttagattc ttttaaaata
     8881 tctattaatt tacttcttct ttcattagaa ttcatattac accttctttc caaataaaaa
     8941 ctttacctat actatataat tttatatttt atcttattat aaatcaatat tgactttata
     9001 ttctatttaa tcttaacttt tataataaat aaaaaagac
//
LOCUS       DAGSRM010000126         8999 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.131, whole genome shotgun sequence.
ACCESSION   DAGSRM010000126 DAGSRM010000000
VERSION     DAGSRM010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8999
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.131"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            177..833
                     /locus_tag="MJE33_003338"
     CDS             177..833
                     /locus_tag="MJE33_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649252.1"
                     /translation="MKKGDFIWSGVLICIIAVLAIPSSREVFMEATGAHPYIGGFLKF
                     AVLATMGDLLGARILYKDWKIPVGVFYRAIVWGIIGVVTTYGMSIYSSGVGQAQVDGM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKIMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
     gene            complement(1310..2581)
                     /gene="sleC"
                     /locus_tag="MJE33_003339"
     CDS             complement(1310..2581)
                     /gene="sleC"
                     /locus_tag="MJE33_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic protein SleC"
                     /protein_id="HBY9649253.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIEAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTAYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRYFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGTDTENAVKIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            2826..3542
                     /locus_tag="MJE33_003340"
     CDS             2826..3542
                     /locus_tag="MJE33_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HBY9649254.1"
                     /translation="MKLDLFLLAIVPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVIILWKINLDKLE
                     KYMNHSKKVFFGNLRKKKKK"
     gene            3621..4931
                     /locus_tag="MJE33_003341"
     CDS             3621..4931
                     /locus_tag="MJE33_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBY9649255.1"
                     /translation="MTELPKEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPE
                     IYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF"
     gene            5031..5567
                     /gene="lepB"
                     /locus_tag="MJE33_003342"
     CDS             5031..5567
                     /gene="lepB"
                     /locus_tag="MJE33_003342"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBY9649256.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDIDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHTI
                     LGKVLVRLYPFSKIGTID"
     gene            5733..6269
                     /gene="lepB"
                     /locus_tag="MJE33_003343"
     CDS             5733..6269
                     /gene="lepB"
                     /locus_tag="MJE33_003343"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBY9649257.1"
                     /translation="MNETIKEEIVEWIKIIITALFFAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            6279..7034
                     /locus_tag="MJE33_003344"
     CDS             6279..7034
                     /locus_tag="MJE33_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HBY9649258.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSELCKY
                     KERISKLGLSIGIHLPMELNTCENIEYIRNSWINFIEKIEFELKGFDLRYFNLHLGYV
                     MTNRVRKNRDKYLGNSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDAYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EVLTLDAEYLVLEINYEDIEDTISKLNNIVGEG"
     gene            7108..8775
                     /locus_tag="MJE33_003345"
     CDS             7108..8775
                     /locus_tag="MJE33_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HBY9649259.1"
                     /translation="MKKINLKIEGKDKEYSLEENSPGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEADVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFVPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDGNKRVVLIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLEE
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
BASE COUNT         3359 a          927 c         1671 g         3042 t
ORIGIN      
        1 gataatggta gatagagtag taagaaaata aatagataaa gggatagaat aaatttaaat
       61 atggaaaatg aatatctaat aataagtgat taataaataa ttgtagatag attgtaaatt
      121 aaataaatct ttaatagaaa ggagaactat ttatatatta aatagtattt ataaatatga
      181 aaaaaggcga ttttatatgg agtggagttc ttatttgtat tattgctgtc ttagcaatac
      241 caagtagtag agaagtattt atggaagcaa caggtgcaca tccatatatt ggaggttttt
      301 taaagtttgc tgtattagct acaatgggag atttacttgg tgctagaatt ttgtataagg
      361 attggaaaat accagtagga gtattttata gagcaatagt ttggggaata ataggtgtag
      421 ttaccacata tggaatgtca atctactcat caggtgtagg tcaagcacaa gtggatggta
      481 tgcttccatt tgagggaata gcttttgcaa atgctctatt taagagtgca actatgaata
      541 tactaatgtc accaactgta tttttattcc ataagattat ggactgtttt atagataaaa
      601 aatatgaggt tggaaagggg attaaggtaa aacttaaaga tgtggtaaat attgtggact
      661 ggaatgctta ccttggtttt agtgttttaa aaactatacc atttttctgg ataccatgtc
      721 atacaattgt atttttattc cctgctgagt atagagtaat tgcttctgca tttgcatcta
      781 tagctttagg tctaatattg gctttagcta ataagtctaa agttgcagtt taggttcact
      841 gatgaagata atagtattat ttaaattagg aataactttt taaattaata ttatttgtat
      901 ttataataaa taataattta tataagaagt aatattctaa gtgatggata gtgttttaaa
      961 taataagaag taatactcta agtgatagat agtgttttaa ataataagga gtaatactct
     1021 aaatgagaag tatcatttta aataataagt gagattttaa ataataagta ggtttttcgt
     1081 tagaatagca ttttattaaa cttaaaaata aatataatat tttaaagaaa tctaagttgc
     1141 taagcactgt ttttaagatt ctaaaaaaac aagttataac taaaaaatgg ctgttaagac
     1201 tacaatactt ataagtaata cttatggtat atattagttc ataacagcca tttttatttt
     1261 aaattaataa ttttattcaa taatttgagt ttatgttcta aatcaagctt taaattaaag
     1321 gatttaaaga agctattcta gttgtagcta cataaactct ggatatttca taccatgttt
     1381 taaaatctac tactccgctt tgaggtaaac caaagattcc ttggaatatc ttaacagcgt
     1441 tttctgtatc tgttccatag ataccatctt ctataacttt tggtacagct ggataagaat
     1501 tagatatagc atttaactga ttttgtatgg ttctaacata ctgcccagaa gaaccaactt
     1561 gtaatgtagt accagggaat gacactggta caccacttac tataggcgct ctttcaaata
     1621 ctatctcatc tccatagaaa tatcttagta tactctcata gtcatatcct tggtcaccta
     1681 agtctttact gccccattga gtcatttggt caggacattg ggatttttgt ccatcacaat
     1741 attgtgctaa tagtggctgt cttgatgtag gaggtctttt tataaaagta ttaaaaatag
     1801 catctacaac tacgttaatt ggttcaaata agtttctatt atttataaac ttatggtcgt
     1861 atgctgttgt tgaagttatt gtaaagttat atcctttatt tctataccat tcagtaaaga
     1921 ctctatttaa agtaaatgat attatagcta ttacatttgc ataaattgtc tgttcaggcc
     1981 aagttgaata tatttcagat gatgctatgt ttttaatgta ctctttaaat ggaatccaat
     2041 agtttggagc tgatgaatct tctggcaaac catcatgaac tactataaat tcaggcacta
     2101 cagggttatc taatacaacg aaaccagtag gaggtggtaa tggttttaag ttactttctg
     2161 gaatcttagg aggataagta ccatatagag tatgttctcc tatgtcaaaa atcaactcac
     2221 tttgtcttga gaaagaacgc ttacttcttg ttctaggaga catgggaact ccttgtcttg
     2281 cttcaattgt agctaacaac tgtgttcctt ctatgacaac tgtctcatat ccatctgcta
     2341 tggcttccac tatatactgg ctataaggtc taacatcaga tggttgtaga gagtaatcta
     2401 catctggtgc tggtaatact aaaccagtca cttgaccaga ttcattactt cttaggtttt
     2461 ggtataaagt tgatgagctt tggctaccat tagacattga atatatgttt actactgcat
     2521 tttgaatagg tctattgtta gtagcatcaa ttatgcttac tgttaagaaa ccatcttgca
     2581 tcaaatcacc ctttctttaa atgaatttag ttcccttata tttcaagata tgaatatagc
     2641 ttaaaatttg ttaaataata tagatataaa tttaaatttt tttgagtatt tgagtatatt
     2701 tgtatttaaa taatttaaaa taatttaatg tatattttag tatgtattta aaacacgaat
     2761 aagttaaaaa taagtagatg atataaatca taaataaatc aaaaattttg aaaggaaaat
     2821 aatctatgaa gctagattta tttttattag caatagttcc tatattgata ggaatgtttt
     2881 ggataaggtc taaagataga tattgtagag aacccctaat ccatctaatt aaattttttt
     2941 taataggtgc ttttctaagt gttataatta tacttttaga aaatttactg atgaagttta
     3001 atgtatttga agggtattca gaactaatat atgtatcatt tgtagttgca ggacttgtag
     3061 aagaaggtgt aaaagctctg atactgattc ctgccctaat taaagaaaaa cactttactg
     3121 aaaagttaga tggtattatt tactctgtat ttttagcttt aggatttgca acaattgaaa
     3181 atatggttta tattttttct gaaagtagaa atctagcact gcaagttggt attaataggg
     3241 cagttatctc tataccagct catgtaatgt ttgcaattac tatgggctac tatatttcta
     3301 agtataaatt tgagggaaat aaaaataaaa gacgtgagta tttgtttatg gcagtgttaa
     3361 ttccaatttt attacatgga gtttttgatt tcatattgat gatagaatat agatgggcta
     3421 ttatattact tatagtttat gtaataattt tgtggaagat aaatcttgat aaacttgaga
     3481 aatatatgaa tcactctaaa aaagtgtttt tcggtaatct taggaaaaag aagaagaaat
     3541 aaaatttaaa ggaaagacat aaataattaa agtataataa atgacacaca agtaaagcag
     3601 attgaagggg gattatagat ttgacagaat taccaaagga atttttagaa gatattgaag
     3661 aaatactaaa agatgaatat gatgatttta taaaaagtta tgaggaaagt aagactactg
     3721 ggcttagaat aaatacttta aaaattgaca agaaaaaatt atttgatttg gagttgtttg
     3781 atttgaatca aataccatgg gcagaagaag gtttttatta tgatgataaa attgacagac
     3841 ctggtagaaa tccactgcat gaggctggag catactattt gcaagagcct tctgcaatga
     3901 gtgtaatacc aaaggcagat attaaagagg gtgaaaaagt tctcgatatg tgtgctgctc
     3961 ctggtggaaa atctacatat atactatcta agctaaacga tacaggttta ttggtttcta
     4021 atgaaattaa tgctagtaga attaaggctc taggggaaaa tttagagcga tttggagcga
     4081 gaaattgtat tataacaaac accgattcta atagcttaag aaagacgttt aatgggtatt
     4141 ttgataaggt tattatagat gcaccttgtt ctgggcaagg tatgtttagg aaggatgagg
     4201 ttgccatatc agattggagt tatgcaaagg tagtagaatg tcaatccatc caaaaagaga
     4261 ttataagaga tggatataaa atgttaaaga aggatggcat tttaatttac tcaacttgta
     4321 ctttttcaag ggaggaaaat gagcatgtta taaatgagtt tatcagtgaa tatccacatg
     4381 cagaacttat tgaaatggaa agattatggc ctcataagat aaagggagaa ggtcattttg
     4441 ttgccaaaat aaaaaaatta gaagaagaag aatgtaatgt caaagaatta aaaataaaaa
     4501 atttagataa agaaataaag gattataggg agtttgaaaa gaaattttta aaggttgatt
     4561 tttatagtaa taaaaaaaat aaattttatt taagaggaga aaatttgtac ctacttcctg
     4621 aaatatatcc agatacgaaa aaactaaaag tattgagata tggtttgcat ttaggggttt
     4681 taaagaagaa tagatttgag ccttctcatg cactatctca ttacttaaaa gttgaagatg
     4741 taaaaaatgt tgaaaacttc agtgtacaga gtgaatcaat tcttaaatat ttaaaagggg
     4801 atgttgtaaa ttctaatgat agtagaggat gggttttggt ttcggttgaa ggaatacctt
     4861 taggttgggg aaaagaatct tctggtgtaa ttaagaatca ttatcctaaa ggtcttagaa
     4921 aagtatttta aactattaga aaaaagaagt ataatattta tattgtaggt ttatggtaga
     4981 ttattttgct gtagattgac ttaatattta acacagagga gacgatttta gtgggtgaag
     5041 cagttaaaaa agaagttgta gaatggataa aagtgattgt catagctctt gttttggcat
     5101 ttgcaataac tcgttttata gtgccaacaa tagtcaaagg agaatcaatg tatcctacat
     5161 tagttgaacg tgattatttg atagttaaca gaattgcgta caaggtagga gagccaaaat
     5221 acaaagatat aatagtattc aaaaccgact taacagagga aaatggaaag aaaaaagatt
     5281 tagtaaaaag agttatcggg gttcctggtg accatgtaaa aatacaagac tccaaggtat
     5341 atgtaaatga taagttgtta gatgagactt cctatataca taataatcgt actgatggag
     5401 atattgatat cgtagttcca gaaggaaaat tatttgcaat gggagataat agagaaaaaa
     5461 gtttagatag tagatacgat gaggttggat tggtcgacga gcataccatt ttaggaaagg
     5521 ttctagtcag attgtatcca ttttctaaga taggaactat tgactaaaca tataaaattt
     5581 ggagcaatcg ctgattagct gaaatcacca gaccatacac aatagttgtc tatataaaaa
     5641 agacaacacc ttatttagta gacttatcta aaagatagtg tttaagagac attatcaata
     5701 aagataatat aactataaag gagaatttgt gaatgaatga aactattaaa gaagagattg
     5761 tagagtggat aaaaataatt attactgcac ttttttttgc atttattata actcgtttta
     5821 taaaaccaac attagtaaat ggagaatcaa tgtacccaac acttaaatca catgattatt
     5881 tggtagcaaa caggatgaca tataagttat cagaaccaaa atgtggagat ataatgatat
     5941 ttaagactga tttattacaa gagaatggaa ggaaaaaaga gcttgtaaaa agggttatag
     6001 gtgttcctgg tgaccatcta aaaattaagg attctaaggt ttatataaat ggtaagttat
     6061 taaatgaagt ttcatatata catgataatt atactgaagg cgatattgat atggtgattc
     6121 ctaagggaaa agtatttgcg atgggagaca atagagaagt tagtttagac agtagatata
     6181 aagaagtggg attagtagat gaagaaaata ttaaaggaaa agttatttta agagtatttc
     6241 cttttacaga tataggtatt tttgagtagg tgatttgtat ggcagttggt atagcgtcct
     6301 tattgtttaa tattgaggag gctttaaaca tttgtgagag tataaagcaa ataactcatt
     6361 tagagattgg aattgacaat atatctgaat gtagtgagtt atgtaaatat aaagagagaa
     6421 tttctaagtt aggattatct atagggatac atttgcctat ggaattaaat acttgcgaaa
     6481 acatagagta tatacgaaat tcatggataa attttattga gaagatagaa tttgaactaa
     6541 aaggatttga tttaaggtat tttaaccttc atttgggata tgttatgaca aatagagtta
     6601 gaaagaatag agacaaatat ttaggaaata gtgtggattt tttagataaa ttaaatacaa
     6661 attcatatgt atgtatagag aatacttatt caaaaggtgg ggatttttca aatattggaa
     6721 atatatctta tgattttgaa tatatattta agaggattaa aaattctaag atttgttttt
     6781 gttacgatac tggacactgt ttaattgatg aggatgctta tgtaaagaac ttaaaggata
     6841 aaataagact gatacactta agtgataatg atggtatcaa tgatacacat gttggtatag
     6901 gaagaggaat tttatctgaa gaggggatta aagaggtctt aacattagat gcagaatatt
     6961 tagtattaga aataaattac gaagacattg aagatactat aagtaaattg aataatattg
     7021 taggagaggg ttaaatacct ctctttatct tgttgatatg cctcattaat taaaatttat
     7081 attcatcaaa aggaaggaga tgttgaagtg aaaaaaataa acttaaaaat agaaggaaaa
     7141 gataaggaat attcattaga agaaaattcc cctggaatca gattgggaga tatagcaaaa
     7201 gaattttgtg atgagcataa gggttatata acacttgcag ttgtagacaa taaactaaaa
     7261 gagctaaatt gtagagttaa gaaggattgt gagataaatt ttttagatac tactaatgaa
     7321 gatggagaga gagtttactt tagagtcatg tcatttatat ttgtaatggc atgtagagaa
     7381 atcttttggg atagcagagt tacaatagaa cattcactat cagatgggct atattgtgaa
     7441 gttcatatag atagaaagct taaagaagca gatgttgaaa atataaaaaa caagatgaaa
     7501 gagatagtaa acaacgatta tgttatagaa aaaattgaag ttacaagaaa tgaagctatt
     7561 tctatctttg aaaataatga aatgtatgaa aaggcagagc ttttgaaata caaagagtat
     7621 gatagtgcta aagtatataa gtgtagaaac tatatagacc atttttatgg ttatatgttg
     7681 ccatcaactg gttatataaa atcatttgat attaagcttt ataatggagg cgttataatt
     7741 ttaggaccta gtgaagaaga taagactctt cctatgaaat ttgtacctca acctaagcta
     7801 tcaagtgtat attatgaagc agaagaatgg tcaagactta tgggaattga caaggtagta
     7861 tctcttaata aaataataga aaacaatgaa tatggagata taataaggac atttgaagca
     7921 ttgcatgaaa agaagttatc ccaaatagcg gatatgataa aggatggcaa taagagagta
     7981 gttttaatag cagccccatc ttcatctgga aaaacaagtt ttgctcacag attatcaata
     8041 catcttagag taaataattt aaatcctatt tcaatttctt tagatgatta ttttatagat
     8101 agaaaacata ctcctttaga tgaatatgga aattatgatt ttgaatcaat ttatgcaata
     8161 gatttagaga agtttaactt agacttaaag aaacttttag ctggtgaaga aattgatgat
     8221 ataagattca attttaaaga aggtaagaga gaatacactg gtaaaaagat aaagatagat
     8281 tcaaatcagc caattatatt agaaggtata catgggctaa atcctatttt aacatcatct
     8341 ataccagatg aagataaatt taagatatac ataagtgccc ttactcaaat aaatttggat
     8401 gaccataatc gaatccctac tgctgatttg agaatgataa gaagaattgt tagagactat
     8461 aattttagag gatatagtgc tgataatact attttacaat gggcatcagt tagaagagga
     8521 gaaaagaaga atatattccc gtatcaagag gaggcagatg ctatatttaa ctctgcttgt
     8581 gtatatgaat tggcagtttt aaaacgattt gcgaaacctt tgttggaaga aataaaggaa
     8641 gataatcctg cacatattga agctactaga ttactaaagt ttttgcagta ttttgttgaa
     8701 ttggacgata cttctgacat accaggcatt tcaattctta gggaatttat tggaggaagt
     8761 aaaatagttg attaatgtgt gtattataga ttgttttatg aagattaaat ggcatagcaa
     8821 gtagtgtgat tggtttatgt taagtatatt aagtgtaaag tggagaatta aattgataca
     8881 gattttaaag tttgagttga tatataaaaa ggcttgtttt atacttttgt atgaagcaag
     8941 ctttttattt taaattttac ttttttgttt aatattttgt tagtttatgt gattatttt
//
LOCUS       DAGSRM010000127         8776 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.132, whole genome shotgun sequence.
ACCESSION   DAGSRM010000127 DAGSRM010000000
VERSION     DAGSRM010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8776
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.132"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            268..1284
                     /gene="gap"
                     /locus_tag="MJE33_003346"
     CDS             268..1284
                     /gene="gap"
                     /locus_tag="MJE33_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBY9649260.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYNDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEKEYDNEKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
     gene            1679..2848
                     /locus_tag="MJE33_003347"
     CDS             1679..2848
                     /locus_tag="MJE33_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649261.1"
                     /translation="MEKLTDRRQVKAVSMETFIFLILLVVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLETVFLIMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIA
                     GAITTYLSDNPAIIPFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEY
                     VLPALIGNLGAIIGSVISVRIMLTFTKKFYKYNPEEDKATGTLEKKDEFREIREGNVF
                     QRALDAILEGGKMGVDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGQEVYTGAAYEGI
                     KLLPVIGDKLGFILEPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIA
                     VFTAMGMCWSGYLSTHIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLVG"
     gene            3228..7481
                     /locus_tag="MJE33_003348"
     CDS             3228..7481
                     /locus_tag="MJE33_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBY9649262.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYEKLGDINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGANQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDENIIFPENAQLYIAIGAGLLSVDEDNIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKINYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINEVYDILPKNIKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEVNHAVDKATDEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANVQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRMRSLKAAMFERESKNIDVKNIKVNRIQYAKNNQITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAIYDAGFKDIPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKSSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEAFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            7630..8124
                     /locus_tag="MJE33_003349"
     CDS             7630..8124
                     /locus_tag="MJE33_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBY9649263.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDGFIVSIYIGDEKFNNLKNECIKK
                     GIDIKRSKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKDIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            8240..8617
                     /locus_tag="MJE33_003350"
     CDS             8240..8617
                     /locus_tag="MJE33_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HBY9649264.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTKAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
BASE COUNT         3551 a          912 c         1598 g         2715 t
ORIGIN      
        1 aaaataagta tagtttaaaa attcaaaaaa aagatggcaa aatatgtccc aagggacatt
       61 gagtaaaata aatgatttca gcttatttac actaaaaagt acatactaaa tgaggggtaa
      121 aataaagaaa aatgatatac taattgtctt ttgataataa aaaaacatgg aatttgatta
      181 tttaatcaag aaaaagtatt gaacaaatga tttatttagt gtacaataat catgtaaaaa
      241 catagtaatg gagggagaca tttcataatg aaaattaagg taggaatgaa cggatttgga
      301 agaataggaa gagctgtatt aagaatagct caagaagaat taggagataa tatagaaatt
      361 gtagccataa atgcaagagc aacaacagaa tctttggcac atttatttac atatgattca
      421 tgctatggaa cttttagagg tgaagtagag gcaaaggatg aagacactct agtagtaaac
      481 tctaaagaga taaaaatatt aagatataat gacccagaag aattaccttg gaaagaattg
      541 ggggttgata tagtaataga atcaacagga ttatttacac aaagagaaaa ggcagaaaaa
      601 catattaaag caggagcaaa aaaagtaatt ataacagctc caggaaaaaa tgaagatata
      661 actatagtta tcggagttaa tgagaaagaa tatgataatg aaaagcacaa tataatttca
      721 aatgcatctt gtactacaaa ttgtctagct ccttttgcta aagtattgga cgaaaaattt
      781 ggtatagtaa aagggttaat gactacagta cattcatata ctaacgatca aagaatatta
      841 gataaaagcc ataaagattt gagaagagca agagcagcgg ctgaatctat aatacctacc
      901 actacaggag cagcaaaagc tgtgtcaaga gtactaccac aattagaagg aaaattaaat
      961 ggtttttcac ttagagtacc aacaccaaca gtttcacttg ttgatttagt gtgcgaactt
     1021 aaagaaaacg ttacatctga gcaagttaac tctgtgttaa aagatgctgc tgaaggagaa
     1081 ttaaaaggtg ttttaggata ttgtgacaag ccacttgttt ctatagatta taaaggagat
     1141 tcaagatcat ctattataga cgctctatcc acaatggtta tagaagacaa tatggttaag
     1201 gtagtttctt ggtatgataa tgaatgggga tattcttcaa gaactgttga cttagtaaaa
     1261 tatgtagctg aaaaattaaa ataatatata tttcttaaag caaattttaa aaaaattttt
     1321 taaaaaaatt atataaaact aaaaaaaaag attatgaaaa atattgaagg aaaaattttt
     1381 tcataatctt ttttctttca tgcaaaaaga tacaagttac aacttatatt tgcatattag
     1441 gtcgttagag aagtatatat atatttagta aggaatcata ctttttataa aatgaaagat
     1501 aagatgtcga aaacttgcat aaatctgaaa aatttttatt attaaaatac tgatttccat
     1561 tttaaaaatg ataaaaaaat gacaaataaa gttgcaaata gtaatactta taagtataat
     1621 taaaaaagag aaaaggtttt gttaaacttt ataacaaaaa aagaagggat gattcaaaat
     1681 ggagaagtta acagacagga gacaggttaa ggcagtaagc atggaaacct tcattttcct
     1741 aatattacta gtagttggtt ttgggtatgt tggtagtatt atgggagcag gaatgatgtt
     1801 caaagtaata atgagtacag cacatgcttt actattggag acagttttct taattatggc
     1861 tatggcagtt cttgcaggcg cattaagcgc actactttca gagtttggag taatagcgtt
     1921 aattaataaa attttcgctg tatttatgaa gcctttatat ggactacctg gtgctagtat
     1981 agcaggagct ataacaactt atttatcaga taatccagct attatacctt ttgctaaaga
     2041 taaaacattc acacagtatt ttaaacaata ccaagttcca gcattatgta acttaggaac
     2101 agcctttggt atgggactta tcttaacaac atttatgatt tctcaaggta cagaatatgt
     2161 acttccagca ttaattggta atttaggtgc aattataggt agtgtcataa gtgttagaat
     2221 aatgttgaca tttactaaga agttttataa atacaatccg gaagaagata aagcaacagg
     2281 aacattagag aaaaaggatg aatttagaga aatcagagaa ggaaatgtat ttcaaagagc
     2341 tttagatgca attcttgaag gtggtaaaat gggggtagat atgggaatgg ctataattcc
     2401 tggagtccta gtagtatgta cattggtgat gttattaact tttggaccat ctacagatcc
     2461 tgttacaggt caagaagttt atacaggagc tgcatatgaa ggaataaaat tacttccagt
     2521 tattggagat aaacttggat ttatattaga accattattt ggatttacat caccagaggc
     2581 aatagcattc cctataactt cattgggagc tgttggcgca gcaatgtctc tagttccaga
     2641 gtttataaag tcaggagcta taactcctaa tgatatagca gtatttacag ctatgggtat
     2701 gtgttggagt ggatatttaa gtacacatat tggtatgatg gatgcattaa atgctagaca
     2761 gttggcaggt aaagctattc tttctcatac aataggtggt ttatgtgcag gagcagctgc
     2821 tcactttata tttacactcg taggataaat gtaatcagct aagataaaaa ataatataca
     2881 aaaaatggtt gttgatatgt aataagaatt tacatatcaa caaccatttt tttctgacta
     2941 aagataattt ataatacata aacattaagt ggatattaga gttaagttat atttatgtaa
     3001 aaagtaacaa aaatctgtag aaatttatga caaaatttag tacactaact atatgttctt
     3061 atttaaaatt taaggttaga attaggaatt acctagtaga gtaagctttg attaataaaa
     3121 gaatcataaa ataaaaatat tagtcataaa gattataaat aacttactta aaataaagcc
     3181 atagaactta caaaaattaa aaaagcatat aaatatggag gggtacaatg gatgaattat
     3241 ttcatatagg aattgatgta ggttctacaa cggtgaaggt tgtggtactt aataatagta
     3301 ataatatagt tcataaagaa tatagaagac attattctga tgtaaaaaaa tcagttaaag
     3361 aagtactaaa tggtatatat gaaaaattgg gagatataaa tactaccatt ataataactg
     3421 gttcaggtgg aattggaata tcaaaaaaat taggtgtaaa atttgttcag gaagttatat
     3481 ctagtacaaa atcaatagag tattttcatc ctgaaactga tgttgtcata gaacttgggg
     3541 gtgaagatgc aaagataaca tatctaagtg gaggaataga ccaaagaatg aatggaatat
     3601 gtgcaggtgg cactggagca ttcatagacc agatggcatc acttttaaag acagatgcca
     3661 gtggattaaa tgaacttgct aaagggtata atgttatata tcccatagct tcaagatgtg
     3721 gtgtttttgc taaaacagat attcaacctc ttataaatga tggagccaat cagactgata
     3781 tagcaatgag tattttcaat gcagttgtcg tacaaactgt gagtgtcctt tcttgtggaa
     3841 gaaaaataga aggaaatgtt gcgtttttgg gtggtccatt gtacttttta tctgaactta
     3901 gagaagcatt taaaagagta ttagatttaa aagatgaaaa cataatcttc ccagaaaatg
     3961 cacaacttta tattgctatt ggagcaggat tattatcagt agatgaagat aatatacaac
     4021 taaaatctct aatagaaaaa ttggatagta taaagaatat tgaagatgga gaagtaaatt
     4081 tattagaacc tttatttaaa gataaaaatg agtatgaaga attttcaaga agacatgaaa
     4141 aagaaaaaat aaattatgtg gacataaatt caataaaaag caattgttat ttaggaattg
     4201 atgcaggctc aactacaact aaagcagcac ttattgatga ggatggaaga cttgtatatt
     4261 cttattataa tagcaatgaa ggaaatccat taaaaacaac cattaaagtt ataaatgaag
     4321 tatatgatat attaccaaaa aatattaaaa tattgagttc aactgtaact ggatatggag
     4381 aaggtcttat taaaaaagct cttaaaatcg ataatggaga gatagagact atagcacatt
     4441 acaaagctgc taaatttttc aacaaagatg tagattttat acttgatatt ggtggtcaag
     4501 atatgaagtg cctaaaaata aaagatggag taattgataa tataatatta aatgaggctt
     4561 gttcatcagg ttgtggctca tttttagaga catttgcaag ttctttatct atgacaatag
     4621 aagaatttgc gcatgaaggt atttattctc aaaatcctgt agatttaggt tcaagatgta
     4681 ctgtatttat gaattctaga gtaaaacaag ctcaaaaaga aggtgcaagt gttggagata
     4741 tatcagcagg gctatcttat tcagtaataa aaaatgcttt atttaaagta ataaagatta
     4801 gagatttaaa tgagattggt aataatattg ttgtacaagg tggaaccttt tataatgatt
     4861 tggtgcttag aagctttgaa aaactaattg gaaaaaatgt aattagacct aatatttcag
     4921 gaattatggg agcttttggt tcagcactta tagctaaaga aaaatatgaa agtggataca
     4981 aaacatcctt gttatctaag gaagagttga ataatatcaa gctagaagca agtgtaacta
     5041 gatgtaaagg ttgttcaaat cattgtcttc ttacaataaa taaattttct gataatgaaa
     5101 tttttatttc aggtaataga tgtgagaaag gtgaagctat ttatggtgat aaaaaaatag
     5161 gattagaaaa gaaaaaacca ataaatttat ttaagtacaa atacaataga atttttagat
     5221 acaaaccatt ggaaaaagaa gaagcaaaaa atggggaaat agggatacca agagtattaa
     5281 atatgtatga agattatcca ttttggttta cttttttcaa tgctatgggc tttagagttg
     5341 tactttcaga taggtcatca aaacagttat atgaaactgg tataactagt attgcatctg
     5401 aaacagtttg ttatccagga aaattagttc atggacatat agaaaattta attgcaaaag
     5461 gtataaaaaa tatattttat ccaagtgtaa caaatgaaaa taaagaggat tcaaatgcag
     5521 ataattatta taattgtcct gttgtaatat catattctga agtaataaaa aacaatgtag
     5581 agaatataag aaataagaac ataaattata taaatccatt tataagtcta aatgacaagg
     5641 aaaaattgaa gaaaagactc tatgatgaat tatcaagaca tttttctgat ataaaaataa
     5701 ctaaagaaga agttaatcat gcagttgata aagctactga tgaacagaat ttatttaaaa
     5761 tggagataca agcagcaggt gaaagagcac ttaaagaaat aaaagaaaga aatatgaagg
     5821 ggattgtgtt atgtggaaga ccatatcata tagacccaga aataaatcat ggaatgccag
     5881 agcttataaa ttcattagat atggcagtgc ttactgaaga ctctatatgt catttagcca
     5941 atgtacaaag accacttaga gttgtagacc aatgggtata tcattcaaga ttgtataaag
     6001 cagctagttt tgtaagagat aaaaactatt tggaacttgt tcaacttaat tcatttggct
     6061 gtggattaga tgcagtaact acagaccagg ttcaagaaat acttaatgaa aaatcgaaga
     6121 tatatactat tataaagatt gacgaaggca acaatttagg agcagctaag ataaggatga
     6181 ggtccttaaa agcagctatg tttgagagag aaagcaaaaa tatagatgta aaaaatataa
     6241 aggtaaacag gatacagtat gctaaaaata atcagataac gaacaaacat actatattag
     6301 caccacaaat gtctccaata catttccaat tccttgaaaa agctgttaac ttaagtggtt
     6361 ataatataga aattctaaaa gatactgata gtagtgtaat tgaagaaggg ttaagatatg
     6421 taaataatga tgcatgttat ccagctataa tagttgtagg tcagctaata acagcattaa
     6481 aatctggcaa atatgattta aataacactt ctgttactat aactcagact ggtggaggtt
     6541 gtagagctac aaattatatt ggattcttaa gaaaggctat atacgatgct ggatttaaag
     6601 atattcctgt cattgcactt agtgtaaatg gaatagaaga tagtggtata atggataata
     6661 tatcactaaa attaatcaat agactcttta tgtctgttgt ttatggtgat ttgctgatga
     6721 aagtactata tagagtaaga ccatatgaaa aagttactgg aagtgcaaat gctctatatg
     6781 aaaaatgggt tgatatatgc aaatcttctc ttgttaaagc aaaaatttct gtatttacta
     6841 gaaatataaa agatatagta aaagaatttg ataatttaga gatactagat atcaaaaaac
     6901 caaaagtagg tttggttgga gaaatattgg tgaaattcca tccaattgca aataataatt
     6961 tagtagatat attagagcga gaaggtgctg aagcagtcgt gccagacctt acaaatttct
     7021 tcttaagttg tgcctttaat actatataca aacacactca tttagaagga agtagaaaga
     7081 gtagaatgat aggagaagca tttatataca taactggaat atatcaaaga gtatataaaa
     7141 aagcacttga taagagtgag agattttatg ctccagccaa tataaaaaat gttgctaaaa
     7201 gtacagaacc agtagtgtca ttaggtaatc aaactggaga aggttggctt ttaacaggtg
     7261 agatggttga attattaaat gaaggtgtag aaaacattat ttgtatgcaa ccttttggat
     7321 gtttacctaa tcacataatt ggtaagggtt ctataaaaga attaaagagg ttgtataaaa
     7381 atgcaaatat aatacctatt gattatgacc cttctgcaag tgaggtaaat caacttaaca
     7441 ggataaaact tatgctttct aaggctttta aaaatatata agatttatat tttgatatgt
     7501 taaaagttaa tgaatttaaa aatatactct atattaagta tctataaaaa agagttatcc
     7561 tattttttag ggtaactctt tattagttaa aaaattatat gtatactgat attaaagggg
     7621 ggaagtaata tgagtttagt atatgatgtt tataaatcaa aattaggaaa tttgtacata
     7681 ttaagtgaag atggttttat agttagtata tacataggag atgaaaaatt taataattta
     7741 aaaaatgaat gtattaaaaa aggaattgat ataaagagaa gtaaggaaga tttaaaggaa
     7801 gcaataagcc agatagatga ttactttaat ggaaaaagaa aagtctttaa tttaaatatt
     7861 cgtattgaag ggactgaatt tcaaaaatca gtttataggg aaatgttaaa aataccttat
     7921 ggagaaacat tgtgttattc agatatagca agaaatataa acaatccaaa atcagtaaga
     7981 gcaataggac aagcaagtaa aagtaataaa attccaataa taataccttg tcatagagta
     8041 gttggcaaaa aagatattgg tggatatatg gggaatcatt ctgatttgaa agagatacta
     8101 ttaaatttag aaagacagag ttaacttaga aagaaatgtt tttattgtta atatttgata
     8161 taataatagt aaaaagaagt attttttaca aaaacaatga agaatttatt ttaatttata
     8221 aaaatgaggg ggaattttaa tgtgtgataa agttatagca tcaaaatatc atgagaaggg
     8281 atttaattgt gcagaatcag ttataaaagc atataatgag gaatttaata cagatatacc
     8341 agtttgttta ggaagtggac tgggttcagg gtgtggggtt gctagtttat gtggtgcagt
     8401 aaatgcatca aatataataa ttggatatgt aaaaggcaga aaccacgaag atgaaagtac
     8461 taaagctaaa gtatatgcta aagatttaac tacaacagta agaaaagagt atggttcaga
     8521 gttatgtata gacctaaaaa aagatttagt tgcttgtaga gaaataatgg atttcgcata
     8581 tgatagtcta aaggaaactt taaaaaaaga attataagaa aataagctat caagtataaa
     8641 ttatttgata gcttattttt atagtcgata ctaaataaat caaatatatt ttagaattat
     8701 tatttgtaaa taaacagcaa ataattttag gatttttatt taatatagtt tttatttata
     8761 tttatttttg aatatg
//
LOCUS       DAGSRM010000128         8745 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.133, whole genome shotgun sequence.
ACCESSION   DAGSRM010000128 DAGSRM010000000
VERSION     DAGSRM010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8745
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.133"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            43..1232
                     /gene="dltD"
                     /locus_tag="MJE33_003351"
                     /pseudo
     CDS             43..1232
                     /gene="dltD"
                     /locus_tag="MJE33_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426716.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
     gene            1198..1338
                     /locus_tag="MJE33_003352"
     CDS             1198..1338
                     /locus_tag="MJE33_003352"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649265.1"
                     /translation="MRDYLKYLKNNKDLLTSICLTMVIILAIGVLTFTPFKELPFIYN
                     GF"
     gene            1353..2867
                     /locus_tag="MJE33_003353"
     CDS             1353..2867
                     /locus_tag="MJE33_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HBY9649266.1"
                     /translation="MKIIEGIKKYSNTDRTALMCNGDKLSYKDLNEYSDAISVFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVLFN
                     FSDERNFGDINVIDMDKLNDIINEYQGKSLDKENWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYRELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPNLESMIFIGEALSKNLT
                     KELMSRFPNTRIINGYGPTEATVGVSVNDMTQKAIDDEKSLPVGYPMSNCKIKILDED
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGVKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNAVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGMIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLMEEI"
     gene            2867..4015
                     /gene="dltB"
                     /locus_tag="MJE33_003354"
     CDS             2867..4015
                     /gene="dltB"
                     /locus_tag="MJE33_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBY9649267.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPAVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEVIIVKGYEYVRRKTKNKYIYWGFLFASMLPIIINKISP
                     VTSFGIIGFIGISYLNFRTIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKQISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSRIPKDMSFINI
                     LSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMITMITMGFWHGLTWCYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKDKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            4034..4264
                     /gene="dltC"
                     /locus_tag="MJE33_003355"
     CDS             4034..4264
                     /gene="dltC"
                     /locus_tag="MJE33_003355"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBY9649268.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            4481..5266
                     /locus_tag="MJE33_003356"
     CDS             4481..5266
                     /locus_tag="MJE33_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBY9649269.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNNTNLDKKQSIAKYAAKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPMSIDTMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKEYIKNSKESFVLCDSSK
                     IGIASMYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            5545..7455
                     /gene="phnW"
                     /locus_tag="MJE33_003357"
     CDS             5545..7455
                     /gene="phnW"
                     /locus_tag="MJE33_003357"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBY9649270.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNIWSDKFGKVPTEDDVNELYAEFEPMLFETLE
                     EYTTPIPHVVETIEKLRKNGLKIGSTTGYTREMMNIVEPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTQ
                     EEVENMDKEELKAKMSIVSKKFKEAGAHFVIETMAELEDILIKIENETIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDVEYNNLVQDVRRRLVSLATQNTDKYTSVLMQ
                     GSGTFSVEAIIGSTISKDGKLLVIANGAYGKRMKDICNYLNIQFVDCTFKDIEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKEYNKIYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEKLSKCKGIAKSLSLDVYAQWETMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKDNGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIEKFINR"
     gene            7814..8362
                     /locus_tag="MJE33_003358"
                     /pseudo
     CDS             7814..8362
                     /locus_tag="MJE33_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426706.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8464..8673
                     /locus_tag="MJE33_003359"
                     /pseudo
     CDS             8464..8673
                     /locus_tag="MJE33_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4004 family protein"
BASE COUNT         3543 a          863 c         1591 g         2748 t
ORIGIN      
        1 taataataac ataaatttga atatttttag aggagagaaa atatgagaaa attgatatac
       61 tttattacac cttttataat aggcgtagta tttttatttg ggttagacaa atttcttgat
      121 tctaaaactg atgaactttt gagggagaaa aatctacttc caattatgga tgatacttta
      181 agtgatataa aagataaagg ggttacagca aataatcatt ttttaagaga aaaggatatt
      241 atgattttgg ggtcatcaga acttagtaat tcaacaaaac aacatccaaa gtactatttt
      301 aatacaaata ggagtaaaaa taaggtgttt gcaattggga gagcctatac tcagacatta
      361 caagatgctg caatattagg aagtatgaat cctaatattg ataataagaa agttgtcttg
      421 ttaatttcta tgcaatggtt tatggaaaaa gacggagtca caagtcacca ttatcagtct
      481 agattttcac caatacaatt ttatcgattt ttagataatc caaaaatatc taaacaaaat
      541 aagattgaat atgcaaaaaa atcaagtaag ttattatggg gaagtgacga gtataaagca
      601 gaagcattat atgctaagct atatgagcct aagacatttc ctgaaaaagc tgaaaaggtg
      661 cttttagagc cttattttca aggaagaaag tattgtatag ctcttaaaga aaagggtatt
      721 ctttataaac gattaattaa gctagataaa aagagagcaa caaaaagaaa aagaccaata
      781 aattggtcat atgagagaaa gaaagccatt gaagatgcta aaaaaagagt tggtaaaaat
      841 ccactaaata ttgataagta ctattataaa gagcatttta aagatggtat agagcaatac
      901 aaaggaagag acaaagatgt aaatttatta acatctaagg agtttgaaaa ttataaactg
      961 atgctaaatg tatgtactga cttaggtatt aagccagttg ttgtattaat cccaagtatg
     1021 gataaattct ataatttgac tggtatttct gaaaaagaaa gaaatcagta ctacgataaa
     1081 gcgcaaaaaa tagcagaaag caaaggcttt gaagttctaa accttaaaga taaagggcag
     1141 ataagtatta tttaagggat gtaatgcatc ttggtacgaa aggatgggta gatgtctgtg
     1201 agagattatt taaaatattt aaagaacaat aaagatttgt taactagtat ttgtttaact
     1261 atggttataa tattggcgat aggagttcta acatttactc catttaaaga attgccattt
     1321 atttataacg gattttaaag gagcattctt atatgaaaat tattgaagga ataaaaaaat
     1381 attcaaatac agatagaaca gcactaatgt gtaatggaga taaactttct tataaagatt
     1441 taaatgaata ttcagatgct atatcagtat ttctaaagga tgtatacaaa gaagaagata
     1501 ctcctatagt aatttatgga aataaagaaa acatgataat ggcatgtatg ataggagctt
     1561 taaaatcagg tagagcgtat gtgcctcttg atattagttt tccaattgat agagtttttg
     1621 aagtcactaa agagataaaa ccaaaggtat tatttaactt tagtgatgaa agaaattttg
     1681 gagatatcaa tgtaattgat atggataaat taaatgatat aataaatgaa tatcagggaa
     1741 aatcattaga taaagaaaac tgggtaaaag atgatgaaaa tgcatatata ctatttactt
     1801 caggaagtac aggaaagcca aaaggggttc aaataagttc aaacaattta gatagctttt
     1861 cagattggat aagcccatat ctaaacatag atggaagtga aaaagtaata atgaaccaac
     1921 cagcatattc ttttgacctt tcagttacaa caatataccc tggtcttatc catggagcta
     1981 cattattttc aatatcaaaa gatgtattag cagactatag agaactattt agacaattta
     2041 gtatctcaga tatagctgta tgggtctcaa ctccttcttt tgcaggagta tgtataactg
     2101 aaaaagagtt taatagtaaa atgcttccta accttgagtc aatgatattc ataggagaag
     2161 cactttcaaa aaatcttact aaagaactta tgagtagatt tccaaacaca agaattatca
     2221 atggatatgg accaaccgag gctactgttg gtgtaagtgt aaatgatatg actcaaaagg
     2281 ctatagatga tgaaaaaagt cttccagttg gataccctat gtcaaattgt aaaataaaaa
     2341 tacttgatga agatggaaat gagcttaaag aaaatgaaaa gggtgaaatt ataataattg
     2401 gaccatcagt ttctaaaggt tacttcaata ataaagaaaa gactgatgaa gtatttttct
     2461 atgatgaaat agatggtgta aagtggagag cttataaaac tggagatatg ggatatcttt
     2521 tagatggaaa tatatattat tgtggaagaa aagattttca gattaaatta aatggattta
     2581 gaattgaaat agaagatata gaaaataatt tgagaaaagt acataatgta aaaaatgcag
     2641 tagttttacc agtatataag gacgaaaaaa ttgcttactt aaaaggtatt gttgagttaa
     2701 atgaaaagaa tgatttaagt aatattaaaa atgggatgat aataaaaaag gaacttggaa
     2761 agtatatacc ttcatatatg attccaagaa atatttcaat aatctctgag tttccaacaa
     2821 atatcaatgg taaaatagac agaaagaaac ttatggagga aatttaatga ttttttcaca
     2881 atatggagat tatttttatc tttatatatt attattaaca tcaataccag cagtaatttt
     2941 aggtctaatg ggcaagaata taaagtatta tggtatgttg gcaagtttgt ttatgatatt
     3001 cttaatagta ggaattgatg tccaactaaa gtacttggta atatttatta ttcttgaagt
     3061 aataatagtt aaaggatatg aatatgtaag aagaaaaact aaaaataaat acatatattg
     3121 gggattcttg tttgcatcaa tgctcccaat aataataaat aaaatatctc cagttacttc
     3181 ttttggaata attggattta ttggaatatc atacttaaat ttcagaacta tacaaatggt
     3241 tattgaaatt tatgatggag ctataaaaga agttaaaatt tcaaagatgc tttattttat
     3301 gttatttttt ccaactttaa gttctggacc tattgatagg tcaagaagat ttgaacagga
     3361 cttggaaaaa caaatctcta gaaaagagta tatagaagaa tacttactgc caggaataaa
     3421 aaatattgtc atgggagtag gttataagtt tgttatagca tttttgataa atacatattg
     3481 ggtatcaaga atacctaaag atatgagttt tataaatatt ttaagttata tgtacgcata
     3541 tagtttatac ctgttctttg attttgcggg atatagttta tttgctatag gtacagggta
     3601 catatttgga atacaagtac ctataaactt tgacaagcca tttatcagca aagatatgaa
     3661 agagttctgg acaagatggc atataagtct ttcaagatgg tttggagatt atattttttc
     3721 tagatttgtt atgtcatcaa tgagaaagaa aagatttaag aaaaggacta ctgctgctca
     3781 tgtggctcag atgataacca tgataacaat gggattttgg catggtctaa cttggtgtta
     3841 tgttgcttat ggtgtatacc aaggattggc acttgtgtta actgatatat atcagcgaaa
     3901 atctaaattt tataaaaaac ataaaaaaga taaatggttc gagagagtgc agattttcat
     3961 tacattccat attgtgtgtt ttggattgct gatattctca ggttatttag cttaatttta
     4021 aggaggaaaa tttatgcaag aaacagttat agaaatattt gaagatgtat tagggactga
     4081 tgagataaga gaagatttag accttaattt atttgagacg gaacttttag actcattagc
     4141 tataattgaa gttttacttg aaattgaaaa tagattaggg atagagcttc aaccaactga
     4201 tttagagaga aaagatatgt ctactgtaaa taatttagta aaattcttgg agacaagaaa
     4261 ataaattagt agttagactt taaatattaa tttataaaat aaaattttac aaagtccatc
     4321 tgatactaat atttgatgga cttttttaat gataatacaa agtcttttga aaataacttt
     4381 aattaagtaa aaaatttaat acataaatat gatttcataa atcttaggta tcattgtaat
     4441 attataggag aaaaatatat tatatttagg aggaaaatga atgtctttaa attcgataga
     4501 tagaaaaaaa gaaatactta aattattaga ttcaaaaggg aaggtaaaca ctaaagaatt
     4561 agtaaaaaga ttagaagtat catcagaaac aataagaaga tatttggaag aattagaaaa
     4621 tgaaaataaa ttaaaaaagg tatatggtgg tgctataaaa ataaattatg gagtgattga
     4681 accagaacat ataaagagaa acaatactaa cctagataaa aagcaaagta ttgctaaata
     4741 tgcagctaag tttgtaaatg acaatgagtc aattatcata gatgaaggta ctaccaattt
     4801 gcagattgta gattatataa tatctgtaaa caatttaaaa attattacaa actcttatcc
     4861 agttctatca aaattgattt cttatgagaa tcaaggtttg tttgatggag aagttatatt
     4921 tattggtgga aaagtaaatt taaaacacca aaggacagca tgtccaatgt caatagatac
     4981 tatgaaaaac ttatacgtag ataaagcttt tatatctagt gaaggtatac ttgatagttt
     5041 tggaattagt agtatagacc aaaataaggc attgttgtca aaagaatata taaaaaattc
     5101 aaaggaaagt tttgtattat gtgatagttc taaaatagga attgctagta tgtataagat
     5161 agcagattta gaggaattag attttgttat ttgtgatgta gaacctccga atgaatggaa
     5221 ggataagtta aatgagataa atataacatg gatagaagca aaataaaccc ttagttttat
     5281 ataatacaac tatgctcaat acaactaaat tcataaaata tagtcagttc atagattaag
     5341 attagaaagt aaattttaga ttgtaactta ggattttatg ggaatatgat acaaaataaa
     5401 aacaactaaa tacaactaaa aacagaaatt taatatatat ttaacctaaa tatgatgtat
     5461 aattaacata tgaaagttat actttaaaag taaaaacaat taaaaacaca aaaattctaa
     5521 aaagaatcta tggaggctat aaatatgaaa aagatatatg gagaaaaaat aaaagcagta
     5581 gtattcgatt gggctggaac aacagtagat tatggatgtt ttgcacctct taatgtattt
     5641 atagagatat ttaaaagaag aggtatagat gtaacaatgg aagaagcaag aaaacctatg
     5701 ggaaaattaa aaatagacca tataagagaa atgtgtgaaa tggatagaat aaagaatatt
     5761 tggtcagata agtttggtaa agtgccaact gaagatgatg taaatgaact ttatgctgaa
     5821 tttgagccaa tgttatttga gacattagaa gagtatacga ctccaattcc tcatgttgtt
     5881 gaaactattg aaaagttgag aaaaaatgga ttaaaaatag gttctacaac gggttataca
     5941 agagagatga tgaacatagt tgaaccaaat gcagcaaaga agggatactc acccgatttt
     6001 cttgtaacac catcagaagt atctcaagga agaccgtatc cttggatgtg ctataaaaat
     6061 gctgaagctt taggggtgtc tcctatgagt tctatggtaa aagtaggaga tacaataagt
     6121 gatgtaaaag aaggcgtaaa tgcaggaatg tggagtgttg ctgtaataaa aggttcaagt
     6181 gagttagggc ttacacaaga agaagtagag aacatggata aggaagaatt aaaagctaag
     6241 atgagtatag tttcaaagaa atttaaagag gcaggtgcac attttgtaat agaaactatg
     6301 gctgagttag aagatatact aataaaaata gagaatgaaa ctattaaatc agattttgta
     6361 ccagaaaatg attacatatt acttacacca ggaccactat ctactactaa atcagtaaga
     6421 gcatctatgt taaaagattg gtgtacttgg gatgttgaat ataataacct agtacaagat
     6481 gttagaagaa gattagtatc tctagcaacc caaaatacag ataaatacac aagtgtatta
     6541 atgcaaggaa gtgggacttt ttcagtagaa gctataatag gaagtactat ttcaaaagat
     6601 ggtaaacttc ttgtaattgc aaatggagca tatggaaaga gaatgaaaga tatatgtaat
     6661 tatcttaaca tacaatttgt tgactgcaca tttaaagata tagaagcagt ggatttaaat
     6721 gtagtagaaa atttattaaa agaaaacaag gatataacac atatatcaat ggttcactgt
     6781 gaaactacta caggtagatt aaatcctata caagaggtag ggaaacttgc aaaagaatat
     6841 aacaaaattt atatagttga tgctatgagt agttttgggg gaattgagat tgatgttgaa
     6901 gattttaata tagatttttt agttagtagt tctaacaaat gtatacaagg agtacctgga
     6961 tttggtttta taatagctaa taaagagaaa ttatctaaat gtaaaggtat agctaaatca
     7021 ttatctcttg atgtgtatgc gcaatgggag actatggaga aaaataatgg taaatggaga
     7081 tttacttcac ctacacatgt tgtaagagct ttttatcaag ctttattaga attggaagaa
     7141 gaaggttctg ttgaaaaaag atatgctaga tataaagaaa atcagtttac aatagcttca
     7201 agattaaaat ctttaggctt tgatactttg gtgaatgata atgctcaatc acctgtaata
     7261 acaactttct tatatcctaa aaatgcaaag tttgaattta tggaatttta tacttattta
     7321 aaagataatg gatttgttat ctatccaggt aaattgactg atatagatac atttagaata
     7381 ggtagcatag gtgaagtcta tccaactgat atggagagac tagctgatgt aatagaaaaa
     7441 tttatcaata gatagaagaa gatagtgtct tgtaggttac agaatagtgc gcaaagccta
     7501 ctcctttagg tgtgtgagat gatagcacaa acagtgtata aaaagtattg acagtagaat
     7561 ttaaagactt atattttaga agtttataca atgaatatat aaacattgag taatagggat
     7621 atattttatt taaaccacaa tctaagaaat tagatttata gtttaaataa agcatttccc
     7681 tattattttt atgcctattt atagaaaata aatgatagtt gctttattaa gtattattat
     7741 catacttgac aacgtaacaa tgttttgtta tattaggttt aaagataaaa tacggtaaag
     7801 aaaggatatc attatgaatg aagaattaag ttcactaaga tttgatggtg ttggaaaatg
     7861 taaaggtgga aattttgata atgttaatat atcaggaaca ggcaaaatag aaggaagtgt
     7921 aaaatgtagt aaatttgatt cttctggttc agcaaaagtt tttgggacaa cagaatgtaa
     7981 tgaatttttt acatctggtt taagtaaagt agaaggaaac atactggcat caaaagttga
     8041 gatatcggga cttcttagat gtacaggaaa tattaattct gttgaaattg atactagtgg
     8101 aattattaat gtagaaggaa gtataaaatc ggatattatt catggagaag gatatttaaa
     8161 agcaaatgag aatatagatt gtgaaaatat caatttaagt ggaatcttgg agtgtaatgg
     8221 atttttgaat tgtgaagaag ttaatattag tttaaatgga acttctaatt ttaatgaagt
     8281 tggagcaagt agtataaata taaaaaaggg gcatggagat tattgcaaaa aatttcgtat
     8341 gctagaaaaa aagcatatct ctagtagata attatgatgt tggaaatatt gaatacagtg
     8401 ataatttatc agtttcagat aattctactg taaaaaacag tataaaggtg ggctagaaga
     8461 gctatggaag aattaatatc taagaaagat ttattgagca aaactaatat atcttatgga
     8521 caattatata gatggaaaag aaaaaacata ataccagagg aatggtttat aaaaaaatca
     8581 gcttttacag gtcaagagac tttctttcca agagataaaa tactagaaag aatagagtta
     8641 atattgtcta tgaaagaaga gacttctctt gattaaaaaa tagctatgca cacttgtgac
     8701 tttagtcatg agttagtagc taaaatagtc agcatatagg gaaac
//
LOCUS       DAGSRM010000129         8159 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.134, whole genome shotgun sequence.
ACCESSION   DAGSRM010000129 DAGSRM010000000
VERSION     DAGSRM010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8159
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.134"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            <1..410
                     /locus_tag="MJE33_003360"
     CDS             <1..410
                     /locus_tag="MJE33_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4004 family protein"
                     /protein_id="HBY9649271.1"
                     /translation="IANMFMKKDINKEFDIDFILSKEVICNYTKEIFQNLYKKEQSIG
                     KKELLILSIIEKFLVKSIITFEELKLIVNIIEENFTSIYNESGKIYLFRKFGVPFVVG
                     CLDYKKVKFEKDVVKIIEVDLTKEINDISIKLS"
     gene            complement(710..1297)
                     /locus_tag="MJE33_003361"
     CDS             complement(710..1297)
                     /locus_tag="MJE33_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBY9649272.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENISISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHIGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(1383..1820)
                     /locus_tag="MJE33_003362"
     CDS             complement(1383..1820)
                     /locus_tag="MJE33_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBY9649273.1"
                     /translation="MEDKYIVHFISKTKASMIKFIENKLSKNGLGELIPTHGNILTAL
                     YENNGILTMKEIAAKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRILKKINDNFKNAD"
     gene            1924..2922
                     /locus_tag="MJE33_003363"
     CDS             1924..2922
                     /locus_tag="MJE33_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HBY9649274.1"
                     /translation="MNKKFKSLYITEEEGNFRRNIVEREVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVKLPKNLSLKEAMDYGTAGFTAALSVYKFIDSVDKNMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKEVLLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMAGKHVGRTVVDLEKI"
     gene            complement(3042..3968)
                     /locus_tag="MJE33_003364"
     CDS             complement(3042..3968)
                     /locus_tag="MJE33_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBY9649275.1"
                     /translation="MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTALYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNMYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            4354..5958
                     /locus_tag="MJE33_003365"
     CDS             4354..5958
                     /locus_tag="MJE33_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBY9649276.1"
                     /translation="MKEKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKSVLDSASSENPILISHASGHMGVANTLGLKQLGV
                     TNETRDPEGGHIGRVEGSEEPNGYLEENAFFNVASKIKQPSSNEIFNSIEKAQNIYLS
                     YGITTAQEGLMEENQFNILKAMANQNKLKMDVVGYVNLKKSKSVADNNREFIKKYINR
                     FKIGGYKIFLDGSPQGKTAWLSRPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAADQLIDAFEKVLNLKEQSSENHIRPVMIHAQTVREDQIDDMKVINMIPSY
                     FVAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAV
                     NRITKKGIQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDENPL
                     TIDPMKIKDIKVLQTIREGEVLYSLK"
     gene            6774..8045
                     /gene="serS"
                     /locus_tag="MJE33_003366"
     CDS             6774..8045
                     /gene="serS"
                     /locus_tag="MJE33_003366"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBY9649277.1"
                     /translation="MLDIKRIRENLDCIKKAMERRGEKDFNLDEVVKLDDERRKILQE
                     VEVMKNELNTASKNIPNLIKEGKDVENEKIKLKELSDKIKVIDQNLKEVEDKMEYLLM
                     RIPNVPHPEVPQGETDEDNVEVRTWGKTTTFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGLDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKAEYVHTLNGSGLAIGRS
                     VAAILENYQQEDGSVVVPEVLRPYMGVSVIK"
BASE COUNT         3141 a          963 c         1282 g         2773 t
ORIGIN      
        1 atattgcaaa tatgtttatg aaaaaggata ttaataaaga atttgacatt gattttattc
       61 tcagtaaaga agttatatgt aattatacca aagaaatatt tcaaaattta tataaaaaag
      121 aacaaagcat aggtaaaaaa gaactgctta tattgagcat aatagagaag tttttagtta
      181 aatctataat aacttttgaa gagttaaaat tgatagtgaa tataatagaa gaaaatttta
      241 catcaatata caatgaaagt ggaaagattt atttgtttag aaagtttgga gttccattcg
      301 tagtaggatg tctggattat aaaaaagtaa agtttgaaaa agatgtagtt aagattattg
      361 aggttgactt gacaaaagaa ataaatgata taagtataaa gttatcatag tgtattatct
      421 tatataatgt attataataa tctacttaaa atacacttta tttaaatatt tagtttagag
      481 atgaggattt atgtgagtat gtaaaagtta aagatagatt taagtaagtt atagggatta
      541 gtaaacaata aaatgaacca tgatgaacta ctataaattg tatttaaact acattttcta
      601 cttatagtaa tatcaatggt tcattttatt gttattaaga taatacagta tttataaaaa
      661 tacttgatat aaatatagta aattgtttaa atacaatgat taccttagtt tattcaaata
      721 atgagttttt aaaatctaag aaatatcctt gtggatgaag acatacagga caagctttag
      781 gagcttcttt acctatatgt atatggccac aatgtgtaca tatccattgt atatcttcat
      841 ttcttctgaa taaagtacct tcttctaaat cagtagcata tctaccaaat ctatcaccat
      901 gaactttttc tatttcagct atattttcaa ataacttagc tatagattca aatccttctt
      961 cacgtgctgt ttttccaaag tttttatata catcatccca ctcttgaagc tcattatgtt
     1021 gtgcagcttt aaggtgttgt gctgtcttgt catataaatc tacagggtat gctcctgata
     1081 tacttatatt ttcaccagca aattctttta atttagaaaa gaattgtttt gcatgtgatt
     1141 tttcttggtc agcagtgtaa ttaaataaag actctattat atataatcct tctttttttg
     1201 ctacagatgc agaaatattg tatctatttc tagcttgact ctcacctgca aaagctctta
     1261 ataaattttc tttagtttta ctatctttta aattcatatt ctacctctct atcacaataa
     1321 atttttacaa tatttgattc tttttattta ttattccaat aggagatata attatgctca
     1381 atttagtcag catttttaaa attatcatta atttttttta aaattctaag aaattcttgt
     1441 ttttcatttt ctgtaatatt atggtatgcc gtctctttaa cttgtgttga gatgctatta
     1501 aaagtatctt ctataagcag tgcctttttt gtaagcttta tatacgtgat acgtttgtca
     1561 tttgtacatt tttgtctttc taaatatcct aggtttataa gtttattaac caacactgta
     1621 acagtggatt tatcttttcc tattttagct gcaatttctt tcattgtaag tattccatta
     1681 ttttcataaa gtgcagttaa aatatttcca tgagtaggta ttaactcacc taaaccattt
     1741 ttactgagtt tattttcaat gaatttaatc atactagcct ttgttttact tatgaaatgt
     1801 acaatatatt tatcttccat aaattattct ccaatcttta atctatttaa attagtttga
     1861 cataaaacta tttataaact ataattagtt taacataaaa ctaaatgtat tgggaggaaa
     1921 tttatgaata aaaaatttaa atcactatat attacagaag aagaaggaaa ttttagaaga
     1981 aatatagttg aaagagaagt ggatacattg ccagaaggcg aagttttaat aaaagtaaaa
     2041 tattcttctt taaattacaa ggatgcactt tctgcaaatg gtaataaggg tgttacgaga
     2101 aagtatcctc atacaccagg aattgatgca tctggtatag tagaatctag tagttgtgat
     2161 gattttaaaa ttggagaaga agttttagta acaggttatg acttgggaat gaatacttct
     2221 ggtggttttt cagaatacat aagagttcct tctagttggg tagttaaact tcctaaaaat
     2281 ctttcactaa aagaagcaat ggattatggt acagcagggt ttacagcagc tttatctgtg
     2341 tataaattta tagattctgt agacaagaat atgggagatg tattagtcac tggtggtact
     2401 ggaggagttg ctgtaattgc aactaaaata ttgattaaat taggttattc agttgtagtt
     2461 tcaacaggaa aattagaaga acaaaaagaa gtattactaa acttaggtgt taaagatgtt
     2521 atacataggt cagaagtgga tgataactca ggaagacctc tgcttagacc aagatgggct
     2581 ggagtaatag atacagtagg aggaaatact ctagcaacag caataaagac aactaattat
     2641 tgtggagcag taacaacttg tggtaatgca ggaggagttg attttacaag ttcagtatat
     2701 ccatttatct taaagggaat tactttatat ggtatagatt ctgtggattg cccaatgaac
     2761 ttaagattaa aaatatggga tttattatca aatgaatgga aattagatga tttagatgat
     2821 atggtaaatg tagtatcatt ggaagagtta aatgatagta tagattcaat aatggcagga
     2881 aaacatgttg gaagaacagt agttgattta gaaaaaatat aggtgaataa atttataatt
     2941 aggattatag gttttaagca aaaatgagat acttgaaatt tacttatgag tatctcattt
     3001 ttttatgtaa aaagatttat gtagatgttt gtgtaaaaag attattcatg aacaattaat
     3061 tcaaatacat ctggtcttga atagtgacct tctgaatcaa aatctagtct actaagtact
     3121 attttttcta ggtctaaatc agctattagc atttcttctt tattgaaaat aggacctgct
     3181 acatattttc caaaagggtc aactatacaa cttcctcctg gacacataat ttctggttct
     3241 gcatctagct ctttatagta gttaaggtca gttggataca tatttttttc aacatattga
     3301 ttgcatccaa ttacaaaaca tcttccttct aaagcaatat gcttcatagt tgcttgccac
     3361 tcttctcttg aatctgcagt aggggctatg taaagtttta cacccttagc ataaagagct
     3421 gttctagcta atggcatata attttcccaa caaatcaacg aacccatttt tccatatgga
     3481 gtatcaacta ctgtaagagt acttccatct ccttctcccc aaatacatct ttctgttcct
     3541 gtaggtttaa gttttctgtg tttaccaatt agcttacctt ctggagagaa aaataagttt
     3601 gtgcaataaa gggtacaatt tatatcatta ccatctcttt ctgttatacc cattgataga
     3661 tatactcctg cttcttgtgc tgcttttcct aataaatctg ttgtagcact aggaactggt
     3721 acagaattat catagtatct tttccaatct tctctacctt ccattgttct acttccaaca
     3781 acaaatccaa atgaaagacc tcttggatat gcaggtatga atgcttcagg gaaaactacg
     3841 atatttgccc cttttctacc agcttcttta attaatccta atgctttttc aacagttttt
     3901 tctaaatcca ttatgactgg tgacgcctgg acaacagcaa ttcttacatt tttattttcc
     3961 ttgctcataa aaaacctcct aaaaaatatt taaatttttt gcattgaata gattacatga
     4021 gatgttattt gagaactaag atacttatat tgtgaataat aatgtaactg tatatttaaa
     4081 ataatacatc atgatttaaa aaattacaat tgtacagata taattgagtt tagatattta
     4141 aatttaacaa cttataatat tttttcaatt agtataaagc ctattaattt gtcttattta
     4201 caatgaatag tatttacttc ttggcaaaca ataattaaaa ataaaacttt tatatgatta
     4261 aactctaaag aaaaagttta taaagaagat aatataatta agcagaaaat aatgaaaaaa
     4321 atttattaaa gtagacaacg taaggagaat tttatgaaag aaaaaattta ttacaatgga
     4381 aatatcatca caatggaaga ttcaatatgt ggagatgcta ttcttataaa agacaaaata
     4441 ataaaaaaga taggaacaaa agaagaagta tttgctttga aaaataaaga tacagaaatt
     4501 atagatttac aaggtaagac attgatgcca tcttttatag attcacatag tcacttgata
     4561 gcttttgcta ctactctaaa gctagttcct ttagaagatg caaccagttt taaagacata
     4621 gtaaaaaaaa tacaggattt caaagagtcg aataatataa aaaaaggaga ctggataatt
     4681 ggattttcat atgataataa ttttttagaa gaaaataagc atccagataa gagtgtactc
     4741 gatagtgcat cttcagaaaa tccaatacta atttcacatg cttctggtca tatgggagtt
     4801 gcaaatacat tggggttaaa acagcttggt gttaccaatg aaacacgtga cccagaaggt
     4861 ggacatatag gtagagtaga aggcagtgaa gaaccaaatg gatatttaga agaaaatgca
     4921 ttttttaatg tagcatctaa aataaaacaa ccatcatcaa atgagatttt taattcaatt
     4981 gaaaaagcac agaacatata tctaagttat ggtattacaa cagctcaaga aggtttaatg
     5041 gaagaaaatc aatttaacat tttaaaagca atggcaaatc aaaataaatt aaaaatggat
     5101 gttgtaggat atgtaaattt gaaaaaaagt aagtctgtag cagataataa tagggagttt
     5161 ataaaaaaat atataaatag atttaaaata ggaggttata agattttctt agatggttct
     5221 ccacaaggta agacagcatg gttgagtaga ccatatgaaa attcagatga tggttattgt
     5281 ggatatccta tatacaaaga tgaagaagta gaaaagttta tagatatttc actaaaagaa
     5341 aagatgcaac tcttaactca ttgtaatgga gatgcagcag cagaccagtt gatagatgcg
     5401 tttgagaaag ttttaaactt aaaagagcaa agtagtgaaa atcatataag accagttatg
     5461 atacatgctc aaacagtaag agaagaccag attgatgata tgaaagtaat aaatatgata
     5521 ccttcatact ttgtagctca cacatattat tggggtgata ttcatatcaa aaaccttgga
     5581 gaagatagag cgtttaagat tagcccactt aaaacaacta tagaaaaggg attaatttac
     5641 actcttcatc aggatacacc agtaatagca cctaatatgc tggaaacagt atggtgtgct
     5701 gtcaatagaa ttacaaaaaa aggtatacaa ataggagaaa atgaaaagat aagcccatta
     5761 gatgctttaa agggtgttac tataaatgca gcatatcaat attttgaaga agataaaaaa
     5821 ggttcaatta aagaagggaa attagctaat ttgataatat tagatgaaaa cccacttaca
     5881 atcgacccta tgaaaataaa agatattaaa gtattgcaga ctataagaga aggagaagta
     5941 ttgtattctt tgaagtagat gaaagttaga tattttataa tctataaaaa atagttttat
     6001 ataataagtt aggtagaatt aaataagatt acagttaaat caacaattat atttttatta
     6061 aattttaatt attgtattga agatttataa gtataatttt attgattcta cctttttaaa
     6121 taactgtaga tttattaaag ttatctatgt actatattaa acaattaagt agttattaat
     6181 tgcacttatt agtttattaa aaaactatgt atattacttt gcagtaagac tttggaacta
     6241 aatctataat agcaaattta aatttattaa atagattacc ttataacatt tcatataaaa
     6301 tatattgtga atctgagaaa attactattt ttctttgagt attttttgta agcagttcta
     6361 aaagattcaa tttcatacct attacttgaa tcattcatta aaataattct aatttaaaaa
     6421 ataattctat ccaaataata gatatatagg actatgttat aatataaaaa aatttatata
     6481 aaagttaata actatataag attgataaag aaaaagtcaa aatagataat tgattagcaa
     6541 aatagatata attataatga agttaaatct agcaatattc tcctaaaatt aaatttcatt
     6601 tatttaatta aatataattt gacaatgggc aaaaaagtga gtaaaatata aataaaataa
     6661 aaataacagg ttcggataaa gaaaaaggtt tcttaacctc aagcataaag tatattatct
     6721 aagttatttg agattaagag ataaatccaa tcttaagtac tgaggaggaa aaaatgcttg
     6781 atataaaaag aataagagaa aatttagatt gtataaaaaa agcaatggaa agaaggggcg
     6841 aaaaagactt taaccttgat gaagtagtta agttagatga tgaaagaaga aagattcttc
     6901 aagaagttga agttatgaaa aatgaattaa atacagcatc taaaaatata ccaaatctta
     6961 taaaagaagg taaagatgta gaaaatgaaa agattaaact aaaagagtta tctgataaaa
     7021 ttaaggtaat agaccaaaat cttaaagaag tagaagataa gatggaatac ctattaatga
     7081 gaataccaaa tgtaccacat ccagaagtac cacaaggtga aactgatgaa gataatgttg
     7141 aagtaagaac ttggggaaag actactacct ttgattttga atctaaggct cattgggaaa
     7201 ttggaacaga attaggtata cttgattttg aaacagctag taaaataact ggttcaagat
     7261 ttactcttta caaaggttta ggtgctagat tagagagagc attattaaat ttttacttag
     7321 acacgaatac aaaagtaaat ggatatacag aagttattcc accatttatg gcaaatagaa
     7381 atagtttttt aggtacagga cagttaccta agtttgaaga agatatgttt aaaatagaag
     7441 gtctggacta ttttatgatt ccaacatcag aagttccttt aactaatata catgctaatg
     7501 aaatacttaa atttgaacaa ttaccgataa attatacagc ttatactcca tgttttaggt
     7561 cagaagctgg ttctgctgga agagatacaa gaggtttagt aagacagcat cagtttaaca
     7621 aagtagaaat ggttaaaata gttgcaccag aagaatctta caatgagtta gaaaagctta
     7681 ctaacaatgc ggaaactatg ctacaattat tgaatctgcc ttatagggta gtaaagatat
     7741 gtacaggtga tttaggattt acggcttctt ttaaatatga tgtagaagtt tggatgccaa
     7801 gttacaatag gtatgttgag atttcatctt gttcaaactg cgaagatttc caagctagaa
     7861 gagcaggaat aagatttaaa agagataaag attctaaagc tgaatatgta cacacattaa
     7921 atggctctgg gttagctatt ggaagaagtg ttgcagctat attagaaaat tatcaacaag
     7981 aagatggttc tgtggttgtt cctgaagttt taagaccata tatgggagtt tctgttatta
     8041 aataagagtt aaattatgta tttttaagca atacaataat atagaggtaa taaaaaataa
     8101 ttaatataaa agtgatactt ctaatataga agtaataaaa aaatagttaa tataaaagt
//
LOCUS       DAGSRM010000130         8090 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.135, whole genome shotgun sequence.
ACCESSION   DAGSRM010000130 DAGSRM010000000
VERSION     DAGSRM010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8090
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.135"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     misc_binding    173..384
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            547..1836
                     /locus_tag="MJE33_003367"
     CDS             547..1836
                     /locus_tag="MJE33_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBY9649278.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGISVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIIATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSTKNKQEIDKAV"
     gene            1890..2969
                     /locus_tag="MJE33_003368"
     CDS             1890..2969
                     /locus_tag="MJE33_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HBY9649279.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     NKSDSKGAVENILKELCGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGDTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            2990..4534
                     /locus_tag="MJE33_003369"
     CDS             2990..4534
                     /locus_tag="MJE33_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBY9649280.1"
                     /translation="MYRVGIDVGGTNTDACILDGELKVIHSVKVATTKDVETGVYNAL
                     KKVIDESKIDHSLIKYAMLGTTHCTNAIVERKRLNNVAMIRIGKPATTAILPFIDWPE
                     DLREKIELDSILVSGGYEFDGRKINELCKDEVVEFCNRIKGKVESVAISGVFAPVNKQ
                     QEEEVAKWVREILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALK
                     DLGIDAQIYFSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGG
                     TTSDLGVLYNGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPES
                     VGYRITEDAIIFGGDTLTLSDVVTKLDMAFEDRKSNVENLDKEYCNEVYKKVILKLED
                     AVDQMKTSQDDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVE
                     KVYSLDKMSREDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVK
                     VVGDLI"
     gene            complement(4697..5239)
                     /locus_tag="MJE33_003370"
     CDS             complement(4697..5239)
                     /locus_tag="MJE33_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HBY9649281.1"
                     /translation="MKSYIALLRGINISGKNKIIMSELKASFVELGYSEVSTYLNSGN
                     VVFLSDVDDSKVISNTIRLMIKKQFELEIPVFIISQEELKDILNNAPNWWGDNNKEIY
                     DNLIFMFPDLSYDEFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYKKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(5387..5671)
                     /locus_tag="MJE33_003371"
     CDS             complement(5387..5671)
                     /locus_tag="MJE33_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBY9649282.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYIL
                     VYRENEDKEKNEHDKNLNETINEFKKEEEKRMQAKSAARAIAEAKKRVLY"
     gene            complement(5734..6498)
                     /locus_tag="MJE33_003372"
     CDS             complement(5734..6498)
                     /locus_tag="MJE33_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBY9649283.1"
                     /translation="MNNYLKFATYQLIGFTSSIPRLIKIKKNPDKFSLKEKFEFMQKQ
                     AKKSLDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVW
                     RNIPIFKEWAKLLRCVYVNRKNNREGIKSIAQASQNILTGQSMAVFPEGDLTWIKEPN
                     SLVSEFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDH
                     IENPRLKSSVLGENIKNKMINTIENFRKSNKTFKEF"
     gene            complement(6780..7046)
                     /locus_tag="MJE33_003373"
     CDS             complement(6780..7046)
                     /locus_tag="MJE33_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBY9649284.1"
                     /translation="MIESRCGILCYECGYKEKVNCKGCTEIEKPFWGEQCPVKSCCED
                     KNLTHCGLCDTFPCEMLNQFAYDKEQGDDGKRISQCKKWAVLAL"
     gene            complement(7088..7897)
                     /locus_tag="MJE33_003374"
     CDS             complement(7088..7897)
                     /locus_tag="MJE33_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY9649285.1"
                     /translation="MNKIINNILNTCKQYTYILPHKLLRQYIAHYTISIPDTSIKENL
                     TLIPDASGCMIFKFDKKGIESAFWGATTKTTIVKNDIENVLFRVFVEFRPGGVYYLTG
                     LSQRESTDLKISLEDFNTLFSLEVNSIFERTSTIKELVEQLDMLFLSYLLKSNIVDMT
                     IPILENARKQNSIMSVKNISQISCYSERHLNRIFNNSLGMSVKSYLRLLRINLVLQEI
                     QNNKIPFATLAQDIGYYDQSHFINDFKSICGVNPTTYIKNLSDFYNEKYKF"
BASE COUNT         2851 a         1009 c         1300 g         2930 t
ORIGIN      
        1 gtataaaaaa aatatatttt gatgaaattt aaaaaaatac attatataaa tatagtaata
       61 gtatgaaaat ttacatataa aactaaaaaa tgcaagtaat tgacttttta agttgacaaa
      121 atattgcata tatattatga tataaatcat aaattaacta caaatcaata ttatatattc
      181 gtagaagaag aaagtagcat aaaggaattt tacagagagt tagatttggc tgagaactaa
      241 tattggaatt tatgtgaaaa ggactttgga gaatactatt gtaattatgt tagaatagtg
      301 gtttaattcg ccttaagaaa atgagtgagt aaaattaatt tttactaata agagtggtaa
      361 cacggattta tattcgtctc ttggattttt ccaagaggcg ttttttatta taaacttatt
      421 tcagaaaatt taaaactata tattaaatga aaattataaa aatatgatag ttaataaatt
      481 tataaattag aaaattgtaa aatatgctaa ggtcagtagt tttacgattt aaatggaggg
      541 atgaaaatgg aagaagaaaa aattaactat gctctggaac agattccaga gaatcaaaaa
      601 cgtggttggg tagctatgtt ttcagtttta gtagcaatag gtgtggattt atcatctgtt
      661 attttaggcg ctgagttggc acagagtatg ccaatgaaac aagcaatatt gtcagtaatt
      721 gttggttcat ttttttctgc aattttgtat actacatgct ctttggttgg ttcatctaca
      781 agtttatcta catctatgat aacaaagtat gtatttggag aagctggagc aaaaatattt
      841 tctctagtta taggggtttc tctattaggt tggtttggag ttcaagtagg tttctttgct
      901 caaaatgcac agataataat aaaagatata tttaatttag atgtcagtat gcagatttta
      961 agcctaatag gtggtctctt gatgatgagt actgcaattt atggatataa ggcaatggag
     1021 aagttgagtg tgtattcagt accattttta ttagttctta tgatgctaac tatattctta
     1081 gcttttagag caaatggaat ttcagttgat gataatatga aatctactat gacatttgca
     1141 ggtggagttt ctctgtcaat gagtataata atagttggtg caattgtatc tcctgatata
     1201 agtcgttggg ctaaaagtag aagagactgt gctctttcaa gctttttggg aatacaattt
     1261 ggaaatgctt ttatgataat agtttcgata gtattagtaa aatgtatggg aacttcagat
     1321 attatgagaa tttttattac attaggaatt gctataccag gtataatagt acttacatta
     1381 gcacaatgga ctacaaatac aagtaatgta tattcagcat cattaagtat agctcttgta
     1441 cttaagaagg ctcctgagaa agtattaact atagtccttg gtattatagc aacattattg
     1501 gctgtatttg gaatatatga aggttttata ggatttttaa atttacttgg aattgtaatt
     1561 gcacctgttg gtggtgtata tacagcagaa tattacatag taaagcaaga acttaaaggc
     1621 tttgataaag gagtattata taaaccaata gttaaacgtt caatagtgtc ttggataatt
     1681 ggtatattaa taacttacct ttcaacatat ggatttataa ctcttactac tatagcacct
     1741 ttagatggtt ttatagcagg gtttgtagta caatctatta ttggaaaagt gctttgttca
     1801 acaaaaaata agcaagaaat agacaaagca gtataaagaa atttgtttat aaagtataaa
     1861 ataaattaga aatatgagga gaaagtaaca tgagattttt aacagaagaa agtgtagata
     1921 aaatagcagt tggtgcagca gtgcttggaa ctggaggtgg aggtgaccca tatgtaggta
     1981 aattggttgc aaaacaagcc attaaaaagt atggtcctgt aaaagtaata agtttagatg
     2041 aacttgatga tgatgctttg gtagttccag tatctggtat gggttctcct gtaattacaa
     2101 tagaaaaatt actttcagaa gtagagttaa ctaccccttt agaaataatg gagaaattat
     2161 taaatagaaa agtggatgtt ataattccta tagaaatagg agggataaac tcacttatgc
     2221 ctatagcagt tgctgctaaa aaaggacttc ctatcttaga tgcagattct atgggtagag
     2281 ctttccctga agctcaaatg gtaacttttt atcttgaagg atatgaggca tctcctgccg
     2341 taatgtctga tgaaaaaggt aattctgcca tcctttatcc tgtggatgga atttggtcag
     2401 aaagattagc tagaacttta actgtagaaa tgggaggaag ttcttctatt agtgactata
     2461 atttaagtgg agctcaagtt aaaaaagctg ctataggtaa tactcttaca atagctgaaa
     2521 ctataggagg gtttttactt gaaaataaat ctgattcaaa aggtgctgtt gagaatatac
     2581 taaaagaatt gtgtggatac aagttatttg aaggaaaagt aattgatatt aagagagagt
     2641 tgaaaggtgg atttactaga ggacatgcct tttttgctgg gattaatgag tatgatggag
     2701 agtattctat attattccaa aatgagaatt taattgccaa aaaaggtgat acaccacttt
     2761 gtattacacc agatttaata gcagtattag atttagaaac tggatttcca ataactacag
     2821 agagaataaa atatggctct cgtgttatgg tagtagcttt tccttgtaat gaaaaatgga
     2881 gaacagaaaa aggaattgag acagttggac ctggatactt tggatatgat gtggaatata
     2941 aaacagtaga agaattacaa ggaaaataaa agtttaggag gaaattttga tgtatcgtgt
     3001 aggaattgac gttggaggaa caaatacaga tgcttgtata ctggatgggg aattaaaagt
     3061 aattcattca gttaaggtgg caactacaaa agatgttgaa actggtgttt acaatgcctt
     3121 aaaaaaagtt atagatgaaa gtaagattga ccattcatta ataaaatatg ctatgcttgg
     3181 tacaactcat tgtacaaatg ctattgtaga aagaaaaaga cttaataatg tggctatgat
     3241 aagaatagga aagccggcca ctactgccat tcttccattt atagattggc cagaagactt
     3301 aagagaaaaa atagaattag attcaatatt ggtatctggt ggatatgagt ttgatggcag
     3361 aaaaataaat gaactttgta aagatgaagt tgtagagttt tgcaatagaa taaaaggcaa
     3421 agtggaaagt gttgcaataa gtggagtatt tgctccagta aataaacaac aagaagaaga
     3481 agttgcaaaa tgggttagag aaattttagg ggatattcct atatctttat caaatgaaat
     3541 aggtaatata ggtatattag aaagagaaaa tggagctatt ttaaattctg cattgtctca
     3601 agtaggtaaa gctgtatctt taggatttga aaaagcactt aaagatttag gtatagatgc
     3661 tcaaatatat ttcagtcaaa atgatggaac attaatgaat ttagagtata caatgaaata
     3721 cccaattttt acaattggat gtggaataac aaattctata agaggagctt ccttcttatc
     3781 aaaagtaaaa aatgcaatag tattagatgt tggtggaact acaagcgact tgggtgtact
     3841 atataatgga ttccctagag aatcatctat tccagttaca attggaggag tacatacaaa
     3901 ctttagaatg ccagatgttt taactattgg tcttgctggt ggtacatgtg taaaaggcga
     3961 aaaagataat ataaagatag gtcctgaaag tgtgggatat agaataactg aagatgcaat
     4021 aatatttggt ggagacactc taactttatc agatgttgtt acaaaattag atatggcgtt
     4081 tgaagataga aaaagcaatg ttgaaaactt agataaagag tattgtaatg aggtatataa
     4141 gaaggttatt ttaaaattag aagatgctgt agaccaaatg aagactagtc aagatgatgt
     4201 tgaattggtt ttaactggtg gaggaagtat aattgttcct gaaaaactaa aaggcataag
     4261 caatgttata agacctcctc attatactgc tgcaaatgct attggtgcag ctttaggaca
     4321 aataagtgga gatgtagaaa aagtatacag tttagataaa atgtcgagag aagatgccat
     4381 agaagatgca aaaaaagagg ctgtaaataa agctctaaaa gctggtgcta aagaagatac
     4441 tatagaagta ttaaatatag aagatgttcc acttgcatac cttcctggta atgcacttct
     4501 tataaaagta aaagttgttg gtgatttaat ttaaagaata aatataagta tttccagaat
     4561 tatataaata tttctggact catttttaaa cttattttat caataaaaac agcataactt
     4621 ttgactatat tcttataagt ttccgaacag ccgatatata cctatttttt gaggtttatc
     4681 ggctgtttgt tatcttttat cgcattccaa ctatttttct taccgtattt gctgtacgaa
     4741 ttgtaatctt atcacttaca tttgaactgg cagtctttga ccaccaattt gtctttttat
     4801 aatctttacg actaaaagac caaaagatag catttttata atgatatact ttttcatact
     4861 cttctttagg atttccaacc tcatcataaa actcgtcata agataagtca ggaaacataa
     4921 aaataagatt atcatatatt tctttgttgt tatctcccca ccaattagga gcattattta
     4981 gtatatcttt taactcttct tgtgaaataa taaaaactgg tatttccaat tcaaactgtt
     5041 tttttatcat taatcttatt gtatttgaga taaccttgct gtcatctaca tcacttaaaa
     5101 agacaacatt accactatta aggtatgtag aaacttctga ataaccaagt tctacaaagc
     5161 ttgcttttaa ttcagacata attatcttgt tcttaccact tatattgata cctcttaata
     5221 atgcgatata acttttcata taataatttc acctctaaac attcacattt ttctatgtag
     5281 tacaaacttg aatttgcatc ttttataatt ctttcaaaac ttcttcatat gtaatttttt
     5341 taatataaaa aaatcggtgc ttaatttgta ccgatttttg tttttcctag taaagaactc
     5401 gtttttttgc ttctgctata gctctagcag cactttttgc ttgcattctt ttttcttctt
     5461 cttttttaaa ttcatttatt gtttcattta gattcttatc atgttcattt ttttctttat
     5521 cttcgttttc tctgtatact aatatataat ctattattga ttcgtttatt atctgagata
     5581 tacttttatt ttcattaatt cctattctct taacttcctt aagtagagtc tcatcaatat
     5641 gaaccgttgt ttgctttgta ttttttgcca ccttaatcac ctcttaaaaa aaattctaac
     5701 attaaaattt tttttagtca ataaatttta atattaaaat tctttaaatg ttttatttga
     5761 ttttctaaaa ttttctattg tatttatcat cttatttttt atattttcac ctagtacaga
     5821 agactttagt ctaggatttt cgatatggtc ataaattggt tctaaaaatt caacttcgac
     5881 aggaacagag ttaattttac caataggttg gtatccttca taagtatctt ttgaattttt
     5941 tattacgagt ggaacaatag ggcattttgc tttatatgct atctttaaag ctccacttct
     6001 aaactccgaa accaatgaat ttggttcttt aatccatgtt aaatctcctt ctggaaacac
     6061 tgccatactt tgacctgtaa gtatattttg agaggcttgg gctatactct ttataccctc
     6121 acgattgttt tttctgttta catatacaca tcttagtaat tttgcccatt ctttaaatat
     6181 aggtatattt ctccaaactg gttcatctgc aattacacat ccaattggtc tttctacact
     6241 tgctgttagt atgaagctgt caagcatact agaatgattt acaacaaata gaagtggttc
     6301 ttttggcagt gtttccttac caattatatt aagctctata ttcactatat ctaaagattt
     6361 tttagcttgt ttttgcataa attcaaattt ttcctttaag ctaaatttat caggattctt
     6421 tttaatttta ataagcctag gtatagaaga tgtaaaacct ataagttgat atgtagcaaa
     6481 ttttaagtaa ttattcataa aattatctcc ttaaaataaa attatttatt actgtaaaga
     6541 ttttaacata aaataaaatt taagtctatt ttaattcaat tttgtaacat atattttaaa
     6601 tagacagaga ttgagatttt atatttccaa ttttagtaat cagattttat caataaatta
     6661 ctattctatt aatttaatta aataaccttt aaatttaatt ataaaaactg ttgcagaaat
     6721 ttctacaaca gtttaaaaaa tttaccttac aaaaacttat tttataacaa gagagtatat
     6781 tataaggcta gtacagccca ttttttgcac tgagaaattc ttttgccatc atcaccttgt
     6841 tctttgtcat atgcaaattg attaagcatt tcacatggaa atgtatcaca tagaccacaa
     6901 tgtgtaaggt ttttatcttc acaacaagat tttacaggac attgttcacc ccaaaatggt
     6961 ttctcaatct ctgtacaacc tttacaattg actttttcct tgtatccaca ttcataacat
     7021 aaaattccac atcttgattc tatcatttta agtcactcct taaatataaa ttttatatga
     7081 ttgtatatca aaatttatac ttttcattgt aaaaatcgga caaatttttt atataggttg
     7141 ttggattaac accacaaata gatttaaaat catttataaa atgagattgg tcgtaataac
     7201 caatatcttg tgctaaagtt gcaaatggta ttttattatt ttgtatttct tgtaatacaa
     7261 ggtttatacg aagtaatcta aggtatgatt taacactcat accaagagaa ttattaaaaa
     7321 ttctatttaa atgtcgttca ctataacaac taatttgtga aatgttttta acagacataa
     7381 tagagttttg ttttctagca ttttcaagaa ttggtatagt catatctact atatttgatt
     7441 tcaataagta tgataagaat aacatatcta attgttctac taattccttt atagtacttg
     7501 ttctttcaaa aatggaatta acctctaagc taaataatgt gttaaaatct tcaagtgaaa
     7561 tttttaaatc tgtagattct ctttgagaaa gacctgtcaa ataatatact ccacctgggc
     7621 gaaactcaac gaatactcta aaaagtacat tttctatatc attttttaca atagtagttt
     7681 ttgttgtggc tccccagaat gcgctttcta tacctttttt gtcaaattta aatatcatac
     7741 atccactagc atcaggtatt aatgtaagat tttctttaat acttgtatct ggaatagata
     7801 ttgtatagtg tgctatatac tgtcttaaca atttatgtgg caaaatatat gtatactgtt
     7861 tgcaggtatt taaaatgtta ttgattattt tattcataag tatatcctcc tgatatataa
     7921 aaaataagta tactaattta ttttttatat aataaaccag ttttgtttta attgaaatat
     7981 tctaataaga tattttgtta aatttagata caataattgt aaagtaaaat ctcaaaatat
     8041 acaattatta taaatcatag atatttatta aataaatata ttaaagaaga
//
LOCUS       DAGSRM010000131         7811 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.136, whole genome shotgun sequence.
ACCESSION   DAGSRM010000131 DAGSRM010000000
VERSION     DAGSRM010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7811
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.136"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            239..1642
                     /locus_tag="MJE33_003375"
     CDS             239..1642
                     /locus_tag="MJE33_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBY9649286.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFVLKKHDIASTYTIASMTPKLNMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     ASLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
     misc_binding    1733..1973
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2016..2714
                     /locus_tag="MJE33_003376"
     CDS             2016..2714
                     /locus_tag="MJE33_003376"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBY9649287.1"
                     /translation="MNVIGIIGAMDEEVSILVDLMDIRETIKKASLEFYKGILEGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELVNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     gene            2737..3738
                     /gene="trpS"
                     /locus_tag="MJE33_003377"
     CDS             2737..3738
                     /gene="trpS"
                     /locus_tag="MJE33_003377"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBY9649288.1"
                     /translation="MSEKKVIFSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANTIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEEIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
     gene            complement(4390..5957)
                     /locus_tag="MJE33_003378"
                     /pseudo
     CDS             complement(4390..5957)
                     /locus_tag="MJE33_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021426623.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iota toxin protein Ib"
     gene            complement(6293..6475)
                     /locus_tag="MJE33_003379"
     CDS             complement(6293..6475)
                     /locus_tag="MJE33_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649289.1"
                     /translation="MKKFRKHKSISNCISILLILYLTLGSLLPNNIYAQDLQSYSGKV
                     CNTTYKAPIERSEGFF"
     gene            complement(6864..7610)
                     /gene="cdtR"
                     /locus_tag="MJE33_003380"
     CDS             complement(6864..7610)
                     /gene="cdtR"
                     /locus_tag="MJE33_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family response regulator CdtR"
                     /protein_id="HBY9649290.1"
                     /translation="MDILIFDNDVCFGMKLKEKINNILIKEGFDNDVVRLYHNANLLL
                     KELTEKNKVRIYFIVVDAKYKISNELCDGLWIAQKIRESDYISPIIFLTNHIEMILGI
                     FDYRLEVMDFILKHDMEIAESKIKACIKIAHKRYIKEKNYRSNFFTIYSDFSLWKISF
                     DEVIYFETSTIPHKIKLVTTSRIFEFYKSLKSLPDLDACFIRVHKSFVVNKYHIVSLD
                     LKKNNIKMSNGHICRISNTYRNILKNIIKT"
BASE COUNT         2807 a          921 c         1130 g         2953 t
ORIGIN      
        1 taataaaata atattgaaac aattttaaaa atttagtatc attagaatta aagtggtaaa
       61 acttttaaat ttatcatttt taataagatt tgatttcaaa gtatttaagg tttattatta
      121 aaactgtttt tgaatatgca aatattttga aaaatatcag tttaataata atgaatctat
      181 tttattaatt ttgaaattaa atttaaaata attaatagag aggggaaatt aaattaggat
      241 ggaaaagaaa aaaataggcc tatggagttt ggtatttatg aatgtatcag ctttatatgg
      301 tataagatgg atagctaaat ctacagcatc aagctttggt ttaggattag gtgcaatacc
      361 tatgtggtta ttattctcat ttatatactt tgtaccagga gctttaattt gtgctgaatt
      421 agcttctaca tatcaaaaag gtgatggagg tctttacgat tgggttaagg aagcctttgg
      481 agaaaagtat ggatttttag tttcttggtt aaactggaca gctaaaatat tttggtattc
      541 ttcattttta actttcttag cagtaaatgt ttcttataca ataggaaatc cagcccttgc
      601 tgataataag atgtttgtac ttatattatc tttaataata ttctgggcat tatctcttat
      661 agcaactaaa ggaataagtt ttacaaaaat atttactaat acaggtgcat taggttcaac
      721 aataccaagt gctatactta taatattttc atttgtagca gtatttgtgt tgaaaaaaca
      781 tgatatagct tctacataca caatagcttc aatgactcca aaacttaata tggatgcatt
      841 tgttgctata tcagctataa tgtttggttt agcaggagca gagactgctg ctaacttcat
      901 aacagaaata gataagcctg aaaagaactt ccctaaggca atattaatat cagctggaat
      961 agtagcttca ttatatgtat taggctcaat tgcaataaca atgataatac caccagacca
     1021 aattactgct tctaaaggtg tattagatgc actttctgct gtttgtgcgt ctttaggaat
     1081 tggttcttgg ttggttcaat taatagcttt tggtatagct ttttcagtat taggagcaat
     1141 agttctttac atagcatcac caataaaaat gttatttggt agtgttaaaa aaggaatatt
     1201 tccagatagt ttagtagagg taaatgaaca caagatacct tctaaagcag ttatacttca
     1261 agcaataata gttactataa tactattggt gactacatta atgccttctg ttgatgctat
     1321 atacaatgtt cttgtaacta tgacagcatt aactgcatta ttcccatatg tattgttata
     1381 tgcttcttat attaaactaa gaaaagagag accagatgaa attagacctt atactatggc
     1441 aaaatcaaca agtacttgcc taggtttagc aaaaatggtt ttagttgtaa ctgtagtggg
     1501 tatattatta tctgcagcac cagttatgcc tacatttgct gaaaatgtta tatatgagat
     1561 agagatgatt ggtggaggtc ttctagttat attatctggt ctatggttat ggaatagata
     1621 tgaaaagaaa acagctaaat aaaatgtatt ttaaaaattt cttttggata ttataatata
     1681 tagttgacaa tttatagtgt aaggaatatt ataaaataaa gaaattaata aattaaatcc
     1741 tgagataaga aatagtaaat aaatatattt ttgcacagag aattaaggat gctgagaact
     1801 taataaaaag tatttattga atgggtcttt gaggagtaaa atgaaatgta atttaatatt
     1861 acagagtatt tgatatcgtg tatgcacgtt atagcatata ttaagatata tatattcaat
     1921 tttgagtgta tataaattta ggtggtacca cggaaaatat atccgtccta ttgatttagg
     1981 acggattttt taatatagaa aatttaggag gaaaaatgaa cgtaatagga attataggtg
     2041 caatggatga agaagttagt attttagttg atttaatgga tattagagag actataaaaa
     2101 aagctagctt agaattttat aagggaattt tggaaggtaa gaatgtagtg ttagtaaagt
     2161 gtggcatagg taaggttaac tctgctcttt gtgcccagat actaataagc gaatttaagg
     2221 ttgatgctat tgttaataca ggtgttgcag gagctttaaa cgaaaaactt gatgttaatg
     2281 atatagtaat atcaactgat gctatacagt atgatgtaga cactacagca tttggagacc
     2341 ctaagggagt tataccaaga atgaagactt ctgtatttaa agcagatgaa agacttatag
     2401 atgctgctta caaatcttct gtggaagaag taaagactca taaagtatta aaaggaagag
     2461 tagttacagg agataaattt ataaattcta aagaattaaa agaagaatta gtaaatgatt
     2521 ttggtggata ttgtggagaa atggaaggtg gagctatagc tcatgtatgc tatttaaata
     2581 atactccatt tgtaataata agagcaatgt ctgataaggc agatggaagt gctgatgtta
     2641 cttatgatgt atttgtacat gatgctgcca ataactctaa agacatagtt ttaaatatgt
     2701 taaaatcaat ataaatatat aaggaggtac aaaaaaatga gtgaaaaaaa agttatattt
     2761 agtggtgcac agccatcagg caaaatgact ttaggaaatt atttaggagc tataaaaaac
     2821 tggactgaac ttcaagacaa ttatgattgt tattatagta tagttgattt gcatgcaata
     2881 acagtacctc aagaccctaa agttttaaga gccaatacta tagaattact tgctcaatat
     2941 atagcttgtg gattagaccc agaaaaaaat actatattta tacaatcaca tgtcaaagag
     3001 catgttgaac ttatgtgggt attgaatact atgacttata tgggtgaatt aagtagaatg
     3061 actcaattca aagataaatc acaaaagagt gaagcaaatc taaatgcagg tttattcaca
     3121 tatccagttc ttatggctgc tgacatattg ttgtatcaaa ctgacctagt tccagttgga
     3181 gatgaccaaa aacaacattt agaattagct cgtgatttag caaatagatt taataataga
     3241 ttttcaccta cttttgtagt tccagaagga tactacccaa aaggtggagc aagagtgatg
     3301 agtttgcaag agccaactaa aaaaatgagt aaatccgatt caaatgaaaa tgcatttata
     3361 ctattagcag atgattcaga ttctataaag agaaagataa aaagaagtgt tactgattct
     3421 ttaggtgttg taaaatacaa tgatgaacaa ccaggtatta aaaatctaat tgatatatat
     3481 tctaatttaa gtaaaaagag tgtagaagag attgtaaaca tgtatgaagg aaagggttat
     3541 ggcatattta aagaagatgt ggctgaagta gtatcagaag ctttaagacc tataagagaa
     3601 aaatatgttg accttcttaa taataaagat tatttagaaa aaatatatag tatgggagca
     3661 gaaaaagccg aaaaacaagc tagaaaaact cttagaaaag tttataaaaa agttggattt
     3721 atagagagaa gatactaatt attagctatc tttacttgat aaataaattt taaagttatt
     3781 aaaaaggtat atgatatgaa agagagagtc tcaaagtgaa cttttgttca tgaggcactc
     3841 ttttttgtat gaaataaaat atattttaat catttcaaca atgaaaaaga ggattatctc
     3901 tattttgaga cagcatcttt tgataataaa aaaagatgag cttttgctca tctcaaaggg
     3961 gttagattat tcttactttg gggaaagtag aataaaggta aaagttaact gatgtcaact
     4021 ttgtcttttt tgcatatcac tactgagcat aaaaaaatca gaaaaccata gccttctgaa
     4081 acaacttcta acatacattg tattttatac ccaatatgta atttatgagt taattttaac
     4141 tcttataatg aaaattgtca ataatatatc aaaaaaatat tttttaaatt tatataatta
     4201 aatgaattta tgtttatata taggtgatat gatacagata attatctttt taatacaata
     4261 tagttctaaa aaagaatata ttagaagaag ataatattta aataaaaaat accttataaa
     4321 gttaattttt taatacttta taaggtaaat acatattgta ttgtggggat aaatttaaat
     4381 catttgatac taatcaacac taagaaccaa taactctcta tcatcttgag taattgtata
     4441 tattcttaat ttcttgtatg tcataatatt ttctccacct gtaaaataac tcctaagatt
     4501 aacacaatca gttttaggtt gatttgtatc tcctaaataa ttacgcaatt ctttatcttt
     4561 agtttcaata tctttaaatc caaaatattg taaagtagct tcaactctat atttttggac
     4621 ataatcgaga gcttctttat tagtttatgt tggtgtaaaa tacataccat ttatataagc
     4681 ctttattgta cttggattaa atttaaaatc tgcagaatat attttatgtg taactattat
     4741 ttcttgaaca gttggaattt taacttctgg ttcatcaagt atttcaggag tactatttag
     4801 ctctgtaata gataagttat ctaagtatat tgtatcacta tcaattaatg ctatatttat
     4861 attagaagaa tctttttcag tagtttcaaa ttcataacta aattttgtat atccttgttc
     4921 tagtaccaaa taatccgttt tttcttcttt tacttttata tttacaatta ttgaattata
     4981 tgttaaagga ttttttgaat tttactaaaa acataacgtt tataaggttt tagttcagac
     5041 ataggtattt taatcttcgt ctcaccattt aatctatttg ctgagccttt taaaccatct
     5101 tgatttgtag tattaacatt actccatata ctagggtaat tattgtaatc atcaaaatta
     5161 gtaaagtaag ttggtgtttt tataagtata ttcattcctc tttcaagttt aacattacat
     5221 atttgtttat cagacaaagt ttttaaactg tctttaatct tattgcctat attatcatca
     5281 aatatgagtt caacacaact ttcatctatt ggtatattat taaaatataa caaaccatct
     5341 tttttagtag tgccaaaagt tttttcaatt gcttctccaa ttgtaagttc aggtgtttta
     5401 tcttctggga cctgtaaact tttagcagta attcttcttt tgtaagattc attttctgta
     5461 tctaatataa tagatacaga aatactgtca atttgaccta tatcgtctta ccaactattt
     5521 cattctgtta ctatttgtcc agaaccattt tttgtaccaa aatttccact tacttgtgtt
     5581 gtttctaatt taatttgctt tccatcatct aattttttta attaattata atttatagga
     5641 atcagtctag aactaaattg atacattgtg ttaagagcca taggtgaaaa tccttttttg
     5701 agataagtat ttccaggaga tagattattg ccaatttgat tttcttgtgc tttgatagtt
     5761 gataatgcat ctccatctaa cactaaattt gttgttggtg tcactttgta cataggtgca
     5821 gtacctgtgt tgtagtatct aatatttgca tttatatatg cagattctcc tttgtttata
     5881 cttaatccag tatttcatga ttatccattg ctattttgaa cagcagttga attatctgtt
     5941 gtatggtaat aatttgtctt gtatagtttc tttaaactca ttaaattttt ctaatgaaat
     6001 ttcgttttga ttttttgttc ttattacttt attaaatgca aatttttctg gagattcaaa
     6061 ataatataca tacataggtt tatctatttt attttttgat atagcatttc gaagttctct
     6121 atattctttt tctcaagaat ttgcttctat ttgataatca taaaaataat ttcttgtctg
     6181 agaatatttg cttatctcta tagaatcttt tttatatgat cctaatactt ctttttcaga
     6241 tttttcaagt ttttgatctt ttctttctca ttctttagcc ttttctttat ctttaaaaaa
     6301 atccttctga tctttctata ggagccttgt aagtagtatt acaaactttt ccactatagc
     6361 tttgtaaatc ttgtgcataa atgttattag gtaacaaact acctaaagtt agatataata
     6421 tcaacaatat agatatacaa ttactaatac ttttatgttt cctaaatttt ttcatttatt
     6481 ctccctacaa atattagttt agttaacttt tgaattttaa tttatttaga ttatataatc
     6541 taaataagaa ttttttatat aaatttcata aaatatgtag gtatatatca taaaaatatg
     6601 tatttatatg atatgtcagt acaaaataac aactgaatat tttaaagaga ctatttcgta
     6661 cttttatgat ttaatgagct atcttatcga atcataatct aggaacaatt gttataatat
     6721 ctaatagtag ttatatatga tatagtcagt atgtgttttt tcacaatatg attattatat
     6781 agaggattct tagggattac tctttttgta taaaaaagag atggttcaaa tttttatctt
     6841 gagacatctc ctttttctat ttattatgtt ttaataatat tctttaaaat atttctatat
     6901 gtattagata tacgacatat atggccatta ctcattttaa tattattttt ctttaggtcg
     6961 agagaaacaa tatgatattt attaactaca aaagatttat gtacacggat aaagcatgca
     7021 tctaaatctg gtaaagactt taaactttta tagaattcaa atattctaga agtagtcact
     7081 aattttattt tatgtggaat agtgcttgtt tcaaaatata taacttcatc aaatgaaatt
     7141 ttccatagtg agaaatctga atagatagta aaaaaattag agcgataatt tttttcttta
     7201 atgtatcttt tgtgagcaat tttaatacaa gcttttattt tactttcagc aatttccata
     7261 tcatgtttta gtataaagtc cataacttct aacctataat cgaagatccc taaaatcatt
     7321 tctatatgat ttgtaagaaa tatgattgga ctaatataat ctgattctct aattttttgt
     7381 gctatccata atccatcaca taattcatta cttatcttat attttgcgtc tacaactata
     7441 aaatatattc ttactttatt tttttcagta agctctttta ataacaaatt tgcattatga
     7501 tataatctta caacatcatt atcaaaacct tcttttatta aaatattatt tattttttct
     7561 tttagtttca ttccaaaaca aacatcatta tcaaagatta atatatccac taaataccct
     7621 cctataaaaa attcaaaagt aaaaaaacaa aagtatttta tagaaaatac ataaaataac
     7681 actaggagat aaataagatt aaggatagtt agaattaagg tatatttatg cttgtttata
     7741 tagtatatct tatacttata gtttacaata ataaagtaaa ttaaatcaat atattattat
     7801 aaaaaattta t
//
LOCUS       DAGSRM010000132         7745 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.137, whole genome shotgun sequence.
ACCESSION   DAGSRM010000132 DAGSRM010000000
VERSION     DAGSRM010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7745
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.137"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            <1..1184
                     /locus_tag="MJE33_003381"
     CDS             <1..1184
                     /locus_tag="MJE33_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBY9649291.1"
                     /translation="IIIIIIFMAYINVVKVFPNIKILLCMVIGISFYITSYEDKIYKS
                     IIVSLLFWLGLMVAEGSAVGLVVFINKLNNINITQHENLFRIQAIIMSKVFLFIGFIL
                     FKYFKLSLDFKPKDMILIGIPILSNIVSLLLIFEYNLRVNTITTENLFIFIFAILLIL
                     SSSIILLIVIGKIVQDDKLNLEYELINERIKTNYRNYENINEVHDRLKYVYHDLKNHM
                     ICIKNYDTKEEIISYINNLEFQISEFEHFKNTGNKTLDLILAEKISICKKYDIQIEDN
                     INISKLNFIKNNDICSIFANSLDNAIEACMDINNEIEKRIEVKATYINKFAIIKFINT
                     KVNDIKLIDKRIQTSKDDDNIHGIGLASIKYIVNKYGGETIVNYSDNEFILKIMIPIK
                     S"
     gene            1196..1336
                     /locus_tag="MJE33_003382"
     CDS             1196..1336
                     /locus_tag="MJE33_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBY9649292.1"
                     /translation="MKKIALNLLKNISALSFGIAVLSANSASSWVAHQAKEPQALQKL
                     KK"
     gene            1395..1976
                     /locus_tag="MJE33_003383"
     CDS             1395..1976
                     /locus_tag="MJE33_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBY9649293.1"
                     /translation="MFKRLSYKFANILVNNEIVESEDFEIYRYGFETLIYFIVNILVA
                     LFIGIIFDRFIHTVIFLSCYCTLRQFTGGYHARNYKECTLTFVVIYLITIFSANNIDI
                     NKYKYLLVLLMIISILTIYKLAPLEHRNKPLSESEKKHYRKTVQKILFVIICLIILCK
                     ILNIFQQYVIYAIISIYWIAILIYIGMKVNSDQ"
     gene            2233..2607
                     /locus_tag="MJE33_003384"
     CDS             2233..2607
                     /locus_tag="MJE33_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649294.1"
                     /translation="MNLKKKIKKLGVTFAIGTIIVSSLGTSVFADEVSTYALISKAFS
                     GKNNIISSSVYTSGSRTFKTTDSQGGFSCRLTERFSGSSPDRIVKRHEANASSKVSLS
                     GGTYYIRYEGSTNNAKTYATLN"
     gene            2690..4399
                     /locus_tag="MJE33_003385"
     CDS             2690..4399
                     /locus_tag="MJE33_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649295.1"
                     /translation="MSSIKVISDFITKIIRKNILFAITIIIINYHLIQRWTHISNRGI
                     SFNDYLSLSVYTCMTISILTMLNVIFYMRKSYDTVKILCRNNFDYFLGISVAVTRNNF
                     IFCCIPIIYAIAYSSLIRTLSIVTIKSTIFILIFEWFLPLTILSIVISFISVIINNLI
                     LKITLSSVFLYLTSTNMLDRVIDIKEPFFKALFKSLNIFEDQSYAYYSDFVGKVNNSA
                     YVIDKIIPTLFAITLIIVAYLILNDIRKKQIKSLIVIVVYSILFIGLNYISIDMSTYR
                     DEERSYGEYLKKSNISDIKIKSHDMKIDFKSKSNIKDRIVLLNTSNEELTNINMILDE
                     IFDIKYIKINSENIKFNKKKDLIEVNLKESLEPNDEVTLDIEYEGYINVLTSWNQNKY
                     IATKDDIMLPPGSLAWYPKVNQSSCMAYKINLSSKNNVYSNLPLLNKTSNFLKKDYIF
                     EGKSNDIAIYAGEFKEEVKDDIKIIYPSDSNVNMDIEEKIKFLIETRNEILGDSKASV
                     DAKKYVDEVDNVMESIKRKKINMILVADLPSNEFLYEDSKGEKISITIYHWLSGNILI
                     IDK"
     gene            4414..5286
                     /locus_tag="MJE33_003386"
     CDS             4414..5286
                     /locus_tag="MJE33_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBY9649296.1"
                     /translation="MINKLEIRNLNFNYKNKKALDNINLTLTDGVVALLGPNGAGKST
                     LMRLLVTLYETSIGEIELNNIKYSKNNEKIKANVGYVPQDFDMYNNINGQEYLEFVAK
                     MRGVSKNDLKKHIQKVVSKVNLDKFINKKIGTYSGGVKRRLGIAQALIGDSKLIVMDE
                     PTVGLDPEQRNEFRRLLPEISKNSIVLISTHIVEDIQFNCDKLIILNQGKILYDGTIN
                     KFIDMVEVYSVVVSNDEFEYLERNIDIIDYRRVKDGVQVKYIGKNTEKLKDSLRIDTT
                     LQDAYINFLHKELK"
     gene            5287..6075
                     /locus_tag="MJE33_003387"
     CDS             5287..6075
                     /locus_tag="MJE33_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649297.1"
                     /translation="MCILELIKFELKKIELKHVLIFVIISILCIFITGILDYNELIFY
                     NDVPIMDTYSIAGWLGIIKISDVFIPIMSAITIIYVFSKDYSGNINEILTLYNKKKYN
                     QFIVFRWCVILAIYTVLLLISITIVYTFTSVKGFNIDKIDFVYSVKPIDVFLKTFPTL
                     LWYTTFPLMILTLTKNRFTTIAILITYTFADIFFILHMYPFVSMINVNGFYIQKMFYM
                     PNNIDVRFNELKNYFFINRGSLVVISIIIVFYIARKSTVLKKSK"
     gene            complement(6171..7004)
                     /locus_tag="MJE33_003388"
     CDS             complement(6171..7004)
                     /locus_tag="MJE33_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HBY9649298.1"
                     /translation="MNVENQSLSDIEIILSHRYDNGADYWTTPDKRLIKGSPFSTLDS
                     VLYLLELGVNPDDTLLKECADLIFSTWREDGRFKLYPNGGIYPCHTANAANVLCHMGY
                     ASDIKMQKTFQHFLDTQYTDGGWRCNKFSFGRGPETEYSNPLPTLNILNAFRFSNYLN
                     KESKLDKAVDFLLDHWTIKKPIGPCHYGIGTLFMQVEYPFRNYNLFLYVYVLSFYNRA
                     KKDNRFLEALKILESKMIGNKIVVERVVPKLSKLSFCKKGESSEIATTHYYEILKNLE
                     K"
     gene            complement(7072..7701)
                     /locus_tag="MJE33_003389"
     CDS             complement(7072..7701)
                     /locus_tag="MJE33_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HBY9649299.1"
                     /translation="MDEKITKNNYNVPFDYVRKKLKELDPHTISNLSNIPFNNDLNRF
                     TLNFFDRQYIIDYPSGEVWNSNGSLCNNFELKILIVRYLINAKGIPRTNKYVTFKEIP
                     GGNVYYPNFLNRTLYRLSEVFTSQIDKYKLVMEKIGAEKVNMGDLAYRFTYMGNTSMV
                     FILWFGDDEFPPNSNILFDSNIVYYFNAEDLAVVPDTAINAILNKINQL"
BASE COUNT         3084 a          782 c         1102 g         2777 t
ORIGIN      
        1 aaataataat tataattata tttatggcat acataaatgt agttaaagtg tttccaaata
       61 ttaagattct tctatgtatg gtaataggaa tatcatttta tataacatca tatgaagata
      121 aaatctataa aagtataata gttagtttat tgttttggct aggattaatg gttgctgaag
      181 gttctgcagt aggtctagta gtatttataa ataaattaaa taatattaac ataacacaac
      241 atgaaaactt atttagaata caagctatta ttatgtcaaa ggtattttta tttataggtt
      301 ttatattgtt taagtatttt aaattatctt tagattttaa acctaaagat atgatactaa
      361 taggaatacc tatattatct aatatagtta gcttattact tatatttgaa tataatttaa
      421 gagtcaatac aattactaca gaaaacttat ttatatttat atttgcaatt ttattgattt
      481 tatcatcaag tataatactt ttaatagtaa tagggaaaat tgtacaagac gataaattaa
      541 atctagagta tgaacttata aatgaaagaa taaaaacaaa ctacaggaat tatgaaaata
      601 taaacgaagt tcatgataga ttaaaatatg tttatcatga cttgaaaaac catatgattt
      661 gtataaaaaa ttatgacact aaagaagaaa ttatatcata tataaataac ttagagtttc
      721 agataagtga atttgaacat tttaaaaata ctggaaataa aacattagat ttaattttag
      781 cagaaaaaat aagtatttgt aaaaagtatg atattcaaat tgaagataac ataaatatat
      841 ctaaattaaa ctttattaaa aataatgata tatgttcaat atttgcaaat tcactagata
      901 atgcaattga agcatgtatg gatataaata atgagataga aaaaagaata gaagttaaag
      961 ctacatatat aaataaattt gcaataataa aatttataaa tactaaggta aatgatatta
     1021 aacttattga taaacgtatt caaactagta aggatgatga taatatacat ggaataggac
     1081 ttgctagtat taaatatata gtaaataaat atggtggtga aacaatagtt aattactcag
     1141 ataatgagtt tatattaaaa ataatgatac caataaaaag ttaggagagt acaaaatgaa
     1201 aaaaatagcg ttaaacttac ttaaaaacat ctcagcatta agttttggga tagctgtatt
     1261 atcagcaaac agtgcaagtt cttgggtagc acatcaggca aaagagccac aagctttaca
     1321 aaaactaaaa aaataacatt aataaaagga cctcttctta ttaaaggaga ggttttacat
     1381 gttgaggagg aaatatgttt aaaagattat catataaatt tgccaatata ttagttaata
     1441 atgagattgt agaaagtgaa gattttgaaa tatatagata tggatttgaa actttgattt
     1501 atttcatagt taatattttg gtagcattat ttataggaat tatttttgat agatttatac
     1561 atacagttat atttttaagt tgttattgca ctttaagaca gttcacagga ggttatcatg
     1621 ctaggaatta taaagagtgt acattaacat ttgtagttat ttatttgata actatttttt
     1681 cagctaataa tatagatata aataaatata agtatttatt agtattatta atgattatta
     1741 gtatactaac aatttacaag ttagctcctt tagaacatag aaacaaacct cttagtgaaa
     1801 gtgagaaaaa gcattataga aagactgtgc aaaaaatact attcgtaata atatgtttaa
     1861 ttattttatg taaaatactt aatatttttc agcaatatgt gatttatgca ataatttcta
     1921 tatattggat tgctatatta atatatattg gaatgaaggt taatagtgat cagtaagatt
     1981 ataaaacatt ttctaaatta attaacttca ctaattaata ttaactttaa aaaaataatg
     2041 tcaaggtaag tatttgaact tattaaatat agttgaatct attgtgggtt gtatcaaagg
     2101 acaaatagat tcacttaata accagtttgt aacagagtat tgaattgtaa aagttaatac
     2161 gatagtatga aaatgtaatt tgaaattaca tttttataaa aattatatta actaagggga
     2221 gggatacata caatgaattt gaagaaaaaa attaaaaagt taggagttac atttgcaata
     2281 ggaactataa ttgtatcaag tttagggaca tctgtatttg cagatgaggt tagcacatat
     2341 gctttaatat ctaaagcttt ttcaggtaaa aacaatatta tatctagttc agtatacact
     2401 tctggaagta gaacctttaa aacgacagat tctcaagggg gattttcttg taggctaaca
     2461 gaaagattct caggatctag cccagataga attgtaaaaa gacatgaagc aaatgcttct
     2521 tcaaaagtta gtttatcagg aggaacatac tatataaggt atgaaggttc aacgaataat
     2581 gctaaaacat atgcaacttt aaattagtat taaaagcatc ctataattat ttttatagga
     2641 tgtttttgta attgttataa tcaaaagact ggctaggagg ggcataaata tgagtagtat
     2701 taaggttata tcagacttta taaccaaaat aattagaaaa aatattttat ttgcaataac
     2761 aattattatt ataaattacc atctcataca acgatggaca catatatcca atagaggaat
     2821 ttcatttaat gattacttat ccttgagtgt atatacttgt atgacaatta gtattttaac
     2881 catgttaaat gtaatttttt atatgagaaa aagttatgat actgtaaaaa tattatgtag
     2941 aaataatttt gattattttt taggtatttc tgttgctgta actagaaaca actttatatt
     3001 ttgttgcata ccaattatat atgctatagc atactcaagt ttaataagaa cattgtcaat
     3061 tgtaacgatt aaatcaacta tatttatatt gatatttgaa tggtttttac cacttactat
     3121 tttatctata gtgatatcat ttatttcagt tattataaat aatttaatac taaagattac
     3181 cttatcatca gtatttttat atttaacatc aactaatatg ttggatagag ttattgatat
     3241 taaagaacca ttttttaaag cattatttaa atcacttaac atatttgaag atcaatcata
     3301 tgcttactat tctgattttg tagggaaagt taataattca gcatatgtta tagataagat
     3361 tatacctaca ctatttgcaa ttactttaat aatagtagca tatctgatat taaatgatat
     3421 tagaaagaaa cagattaaat cattaatagt tatagtagtg tacagtattt tatttattgg
     3481 attaaattat atatcaatag atatgtcaac atatagagat gaggagcgta gttatggaga
     3541 gtatttaaag aaatctaata ttagtgatat taaaataaaa tcgcatgata tgaagattga
     3601 ctttaaaagc aagtcaaata taaaagatag aattgtttta ttaaacacat caaatgaaga
     3661 gttaacaaat attaatatga ttttagatga aatatttgat ataaaatata taaaaataaa
     3721 ttcggaaaat ataaaattta acaaaaaaaa ggatttaatt gaagttaatt taaaggaaag
     3781 tttagagcct aatgacgagg tgacattaga tatagagtat gaaggctata ttaatgtgtt
     3841 gacatcttgg aatcaaaata aatatatagc tactaaagat gatattatgc ttccaccagg
     3901 ttctttagcg tggtacccaa aagtaaatca atcaagttgt atggcttata aaataaattt
     3961 aagttctaaa aataatgtct attcaaattt acctttactg aataagacta gtaatttttt
     4021 aaaaaaagat tatatatttg aaggaaagtc aaatgatatt gcaatatatg caggtgagtt
     4081 taaagaagag gtaaaagatg atataaaaat aatctatcca tcagatagta atgtaaatat
     4141 ggatatagag gaaaaaatta aatttttaat tgaaacaaga aatgaaatat taggtgatag
     4201 caaagcatct gtagatgcta aaaaatatgt agatgaagta gataatgtta tggaatcaat
     4261 aaaaagaaaa aaaattaata tgatattagt tgctgactta cctagcaatg aatttttata
     4321 tgaagactca aagggagaga aaattagcat aactatttat cattggcttt caggaaatat
     4381 ccttataata gataaataat aaaggaggtt aatatgataa acaagttaga aataagaaat
     4441 ctaaatttta attacaaaaa taaaaaagct ctggataata taaatttaac cctaacagat
     4501 ggagtagttg cattattagg tcctaatggt gctggaaaat caacattaat gaggttatta
     4561 gtgactcttt atgaaacttc aattggagag atagaattaa ataatataaa gtatagcaaa
     4621 aacaatgaga aaatcaaggc aaatgtagga tatgtacctc aagattttga tatgtataat
     4681 aatataaatg ggcaagaata cttagaattt gtagctaaaa tgagaggtgt atctaagaat
     4741 gatttaaaga aacatataca aaaagtagta tcaaaagtta atctggacaa gtttataaat
     4801 aaaaaaatag gtacatactc aggtggtgta aagagacgac taggtatagc acaggcttta
     4861 ataggtgatt ctaagttaat agttatggac gaacctacag ttggtcttga tccagagcaa
     4921 aggaatgaat ttagaagatt gttaccagaa ataagtaaaa attctatagt attaatttca
     4981 actcacatag tagaagacat acaatttaac tgtgacaaat taattatatt aaatcaaggc
     5041 aaaatattat atgatggtac aattaataag tttattgata tggttgaggt gtattcggta
     5101 gtagtttcta atgatgaatt tgaatattta gaacgtaata ttgatattat tgattatagg
     5161 agagttaaag atggtgtgca agtaaagtat ataggtaaaa atacagagaa acttaaagat
     5221 agcttaagga tagatacaac tttacaagat gcttatataa actttttaca taaggagtta
     5281 aaataaatgt gtatattgga gttaataaaa tttgagctta aaaaaataga gcttaaacat
     5341 gtgttaatat ttgtaattat ttctatatta tgcatattta ttacaggcat attagattat
     5401 aatgagttaa tattttataa tgatgtacct ataatggata catattctat agcaggatgg
     5461 ttaggaatta taaagatatc agatgttttt ataccaataa tgtcagcaat cacaataatt
     5521 tatgtgtttt caaaagatta ttcgggtaat ataaatgaga tacttacttt atataataaa
     5581 aaaaaatata atcaatttat agtatttaga tggtgtgtta tattagctat atatactgta
     5641 ttattattaa tttcaattac aatagtatat acatttacgt cagtgaaagg ttttaacata
     5701 gacaaaatag attttgttta tagtgtaaag ccaatagatg tgtttttaaa gacgtttcct
     5761 acattattat ggtatacaac atttccacta atgatattaa cattaacaaa aaatagattt
     5821 acaacaatag caatattaat tacatataca tttgctgata tattttttat tttacatatg
     5881 tacccatttg ttagtatgat taatgtaaat ggattttata ttcaaaagat gttttatatg
     5941 cctaacaata tagatgttag atttaatgaa ttaaagaatt atttctttat aaatagaggt
     6001 tctctagtag ttatttctat aataatagtt ttttatatag caagaaaatc aacggtttta
     6061 aaaaagagta aatagtttaa tatatttttt atattgttat atttatatag aatagtgact
     6121 ttaaaaaatt taaagccact attttttaat atgttttatc tatataacta ttatttttct
     6181 aggtttttta atatttcgta atagtgtgtt gtggctattt cacttgactc gcctttttta
     6241 caaaaagata atttagatag ttttggaact acacgttcaa cgacaatttt attacctatc
     6301 attttagatt caagtatctt caatgcttct aaaaatctat tatccttttt tgcacgatta
     6361 taaaatgata aaacgtagac ataaaggaat aggttataat tacggaaagg atattcaact
     6421 tgcataaata atgtgccaat tccatagtga caagggccga ttggtttttt aatagtccaa
     6481 tggtcaagta gaaaatctac agctttgtca agttttgatt ctttatttag ataattacta
     6541 aaacggaatg catttaatat attaagagtt ggtagtggat ttgagtattc agtttctgga
     6601 cctcgaccaa aactaaattt attgcaacgc cacccaccat cagtatactg tgtatctaaa
     6661 aaatgttgaa acgttttttg cattttaatg tcagatgcat atcccatatg acagagtacg
     6721 ttagcagcat ttgcagtatg acaagggtaa atacctccat ttggatatag tttgaaacgt
     6781 ccatcttctc gccatgtgct aaatatcagg tcagcacatt ctttaagtaa agtatcatca
     6841 gggttcacac ctaattctaa tagataaagt acactatcaa gagtagagaa aggagaacct
     6901 tttattaaac gcttatcagg tgtagtccaa tagtctgcac cattatcata cctgtgtgat
     6961 aatattattt caatatctga taaagattga ttttctacat tcatttttta ctccatttca
     7021 tattatattc tattaaatct atcaaaaact ttattataat attatttata tttataattg
     7081 atttatttta tttaaaattg catttatagc tgtatcagga acaactgcta aatcttctgc
     7141 attgaagtaa taaactatat tagaatcaaa taatatattt gaatttggag gaaattcatc
     7201 atctccaaac caaagtataa ataccataga tgtattaccc atatatgtaa acctataagc
     7261 taaatctccc atgtttactt tttcagctcc aattttctcc ataactaact tatatttgtc
     7321 tatttgagat gtaaaaactt ctgaaagcct atatagagtc ctgtttaaaa aatttggata
     7381 ataaacattg cctcctggta tttctttaaa agttacatat ttattagttc tagggatacc
     7441 tttagcattg attaaatatc taactatcaa gattttaagc tcaaagttat tgcaaaggct
     7501 accattacta ttccaaactt ctccacttgg atagtctata atatattgtc tatcaaaaaa
     7561 attgagtgtg aatctattta aatcattatt gaaaggtata ttacttaaat tactaattgt
     7621 atgagggtct aattctttga gtttttttcg aacatagtca aatggaacat tataattatt
     7681 ttttgttatc ttttcatcca taattttttc ctccaattta aacaattaaa agtatattta
     7741 taaaa
//
LOCUS       DAGSRM010000133         7660 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.138, whole genome shotgun sequence.
ACCESSION   DAGSRM010000133 DAGSRM010000000
VERSION     DAGSRM010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7660
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.138"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(66..590)
                     /locus_tag="MJE33_003390"
     CDS             complement(66..590)
                     /locus_tag="MJE33_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY9649300.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRTYSVMRNTLALMSDRVDKT
                     EAMLASLGENDYMFVAETNNNGEKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLTRVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(643..867)
                     /locus_tag="MJE33_003391"
     CDS             complement(643..867)
                     /locus_tag="MJE33_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649301.1"
                     /translation="MIIKLTKDNFNFRFKQYEGMFFHAEVVEGMESIRYKITKDKEGT
                     ELKEFGSTHIKDNMIYVPQVEAYIDLNSIK"
     gene            1064..2188
                     /locus_tag="MJE33_003392"
     CDS             1064..2188
                     /locus_tag="MJE33_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HBY9649302.1"
                     /translation="MSKKTKYFSLILFPIALLLNFIASKVPNIVEKYYSQFIDKIIVQ
                     ILSKVSGIFPFSLYEITMYIIVISIFLFLCCTLSTIFNKKKKLNIYLKNSILNILSIV
                     SIVYFLFVVLWGINYNRIPLETTLINNYNLKNNTSIQSKQYSVKELTKLYKFLVIKAN
                     ETRKLTLQDKNGIVKSNTDYNGVINRAQLGYDNILDILPGVSGSYSKPKYIISSNLMC
                     YTGITGIYFPFTGEANVNIAIPDLYIPCTVEHEMAHQRGFASEDEANFIAYLTSIKHP
                     NIDFNYSGYILALNYTASALSKVDYNAYVDISAGISDSVRRDLKNESEFWQKYEGKIN
                     EISNEFNNSYLKANSVTEGTQSYGKMVDLLLTYYELYPYN"
     gene            complement(2662..3486)
                     /locus_tag="MJE33_003393"
     CDS             complement(2662..3486)
                     /locus_tag="MJE33_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649303.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTNVYLANTVS
                     TVTKKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLELYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSVWKNSAYENWLKSFQGKEVSNSVLENYYK"
     gene            complement(3834..4889)
                     /gene="ispG"
                     /locus_tag="MJE33_003394"
                     /gene_synonym="gcpE"
     CDS             complement(3834..4889)
                     /gene="ispG"
                     /locus_tag="MJE33_003394"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HBY9649304.1"
                     /translation="MSYERRKTREVSVGTVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIEQGVDGIR
                     INPGNIGSIERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKYGSATAEALVESAMGH
                     IKILEDLDFHNIVISLKSSDIYKTVDAYELISKKVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
     gene            complement(5071..6075)
                     /gene="rseP"
                     /locus_tag="MJE33_003395"
     CDS             complement(5071..6075)
                     /gene="rseP"
                     /locus_tag="MJE33_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBY9649305.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGELKLSITRDGSDKVVNVTPKNNNGKYEIGIQPQREKDFLGSIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            complement(6088..7242)
                     /locus_tag="MJE33_003396"
     CDS             complement(6088..7242)
                     /locus_tag="MJE33_003396"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HBY9649306.1"
                     /translation="MKKISILGSTGSIGKQTLDVVRDNRDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEMLPKNIEIEVLAGMEGLKIISSLDEVDVLLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELANITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFGVEQENIDVVVHPQSIIHSMVQYTDSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            complement(7406..7609)
                     /locus_tag="MJE33_003397"
     CDS             complement(7406..7609)
                     /locus_tag="MJE33_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY9649307.1"
                     /translation="MISYAPLWKLLIDRKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKSI"
BASE COUNT         2511 a         1369 c          763 g         3017 t
ORIGIN      
        1 aatagattat ctttaatgta aattttttat taaagacact ctattttact tctctattag
       61 cttatttaaa ttaaattttt attatatctt cccataatat aaacatcttc aaattttcca
      121 tctctgatta cagcatattt tttagttccc tctatttcaa aacctagctt tttatataaa
      181 tttaatccct tttcattatc cacaattact tctaatccta ctctagttaa cataatccaa
      241 ttatctgcta tatctaaaag ttgttccata agcttcttac ctactcctct tccctgataa
      301 ttctctgcaa cacttattcc tatttctgcg ctatgtctca atcgtggaga agaatttaca
      361 tgtaacccag caaaacctat tacatctttt tctccattat tattagtttc agctacaaac
      421 atataatcat tttctcccaa agaagctaac atagcttcag ttttatcgac tctgtcactc
      481 attaatgcca aagtatttct cataacactg tatgttcttc taatttcatt aaaagcttgt
      541 gcatcttctc ttcttatagg tcttattgtt atatctatag gcttactcat attggattcc
      601 ccctcaaaca taatctttat ttttaattaa aattttacat ctttatttta tactgttcaa
      661 atctatgtat gcttctacct gaggtacata tatcatatta tcttttatat gtgtacttcc
      721 aaattctttt aattctgtac cttctttatc cttagttatt ttatatctaa tggattccat
      781 accttcaact acttcagcat gaaaaaacat accttcatat tgtttaaatc taaaattaaa
      841 attgtcttta gtaagtttta ttatcatatt tttatctact ccttatataa ttgatatttt
      901 tcttattatt agttgtatat atactgctta ttcatattaa tatattataa cttatcattt
      961 atttagtata aagttataaa tagatatatt tatacttatt ttacaatctt tcttcaattg
     1021 atatataata tgtttatata aactaatgta tgtgaggtga aatatgagca aaaaaacaaa
     1081 atatttttct ttaattctct ttcctattgc tttgctttta aattttatag ccagcaaagt
     1141 tccaaatatt gttgaaaaat attactccca attcatagat aaaataattg tacaaatatt
     1201 gagtaaagtt tctggaattt tcccattttc tctctatgag attacaatgt acataatagt
     1261 tatatctatc ttcttatttt tatgttgtac tttatctaca atttttaata agaaaaaaaa
     1321 attaaacata tatttaaaaa attccatttt aaatatatta tccattgtat caatagtcta
     1381 ctttcttttt gtagtgcttt ggggaataaa ctataatagg attccactag aaaccacact
     1441 tataaataac tataatttaa aaaacaatac ctctatccaa tcaaaacaat atagtgttaa
     1501 agagcttact aaattatata aattccttgt aattaaagca aatgaaacta gaaaactcac
     1561 attacaagat aaaaatggta ttgtaaaatc taatacagat tataatggtg tcataaatag
     1621 agctcaacta ggatacgata atatcttgga tatattacct ggtgttagtg gtagttattc
     1681 taagcctaag tatattattt catcaaatct aatgtgctat acaggaataa ctggtatata
     1741 ctttcctttt acaggtgagg caaatgtaaa tatagcaata cctgacttat atattccttg
     1801 tacagttgaa catgaaatgg cacatcaaag gggatttgct agtgaagatg aggctaattt
     1861 tatagcgtat cttacttcta taaaacaccc caatattgat tttaactatt ctggatatat
     1921 tttagcttta aattatactg cttctgcttt aagtaaggtt gattataatg catatgttga
     1981 tatttctgct ggcatttcag attctgtccg tagagattta aaaaatgaaa gtgaattttg
     2041 gcaaaaatat gaaggtaaaa ttaatgaaat ttctaatgag tttaacaatt cttacttaaa
     2101 agctaatagc gttactgaag gtactcagag ttatggtaaa atggttgatt tattgctaac
     2161 ttattatgaa ttatatccat ataactaaac actacaattc aaaattttat tcatatacag
     2221 ctgatattaa atcagctgtt ttctatttaa ataaattatt taacttcaag caatcttata
     2281 taatttgata gtacttatac tatctgtata acatttcaat tctaactatt ttagttaaaa
     2341 catttctatt atctttttta ataaatgttt taactaaaat ttttaaaaca taaaacttcc
     2401 acattatttc actatctata taaaaatcta aaaattcaca tataaaatta cataactaaa
     2461 aaatcataaa tttatagaaa acatatattc aaaataaaaa agcatgtatc aaaacttgac
     2521 acatgcttat aatcttatgt atatatatca ttttgtcaca ataaaaattt acttgtaaca
     2581 ctgatatttt taaacaaaat agtccttatt taattaaatt aacattaatt aaattcgtac
     2641 tcattttgct atcattaaac attacttata gtaattttct aatacactat ttgaaacttc
     2701 cttcccttga aaacttttca accaattctc atatgctgag tttttccata cactaccatc
     2761 attaaatgtt acttgtttta cacaagatat aatgtacttt acaccatgct ctccagttga
     2821 ttgtttccat ttatcaaaca atagaaaggc atcattttga ttcttacctg gtgctattgc
     2881 attttgcttt gcatattctt tctccatttg tttccattct ttcaaccaat tttctaccca
     2941 tgccttgcct tgttgttttt caaacatact tattatatcc tgtggtggtg attgttgcat
     3001 ttttctataa gtatgtgaat atgattttgg agacacagga gatatgcctg taacaatacc
     3061 atagtctaca ccagctgggc taaatccaac acttcctact tcgccatctg ctgccacatt
     3121 ttgagcatcc caatatagct caagtggttt tccattttta tcatatgcta agcatgtaat
     3181 ttcataatcc ataattgcct tttcactgct atttgttaag cttataacat tgctaggcca
     3241 atgatatgga gtatcatcat actgtatttt gctattattt ataaataatg gaatattctc
     3301 attataaaca agacctttac tactttgaat atctgatacc ttctttgtga cagtacttac
     3361 cgtatttgct aagtatacat ttgttttttc tgaattggtc gtactaagag aaaatgttgt
     3421 tgtcgttcca gtaatcagca aagcaacaac taaaataggt aagctagata tttttttata
     3481 tctcacaatt cttcacctct ttttctatta acttataata aaacaattta ccaaaacatt
     3541 aaaatctatg ttaacataat ttaccaactg ttctagtaac tatatcctat catagttact
     3601 atcaagtttc aatactattg atggaaattt tcactatatt aaatcttttt ttaattttat
     3661 aaatttacaa tatgatttaa ctcatacata tatagtcaaa ttagaatata agtttcttac
     3721 atttaatgaa gtttataaaa taaaaaatga ctagataatt aaatactgta aataaaaact
     3781 tattacaaaa gtatttaaaa atctagtcaa ttaatatata tatgaattgt atactataat
     3841 ttttcaatct cttctagcaa ctcatcaact aatttgttac catcaatcat tctaactatt
     3901 tcaccctttt taaataaaag accttcacct ttacccccag caatacctat atctgcctct
     3961 ctggcttcac caggtccatt tacagcacag cccataattg caacagtgat atttttttcc
     4021 atactaccaa tcttttcttc aacctcattt acaacactaa ttaagtctat attacatcta
     4081 ccacatgtag ggcatgatat aactttaatc ttatcattta aaagtcccaa acttcttaaa
     4141 atctgtttac caacaataac ctcttcaaca gggtcgcctg ttaaagatat tctaacagta
     4201 tctccaattc ctttaagaag aagtgctcct acccctatag aagactttat tgttcctttg
     4261 tgaacacttc cagattctgt aattccaata tgaagaggat aatcaacttt ttttgatatt
     4321 aattcatatg catcaacagt tttataaata tcggatgatt ttagagatat aactatatta
     4381 tgaaaatcta agtcttcaag aatcttaata tgtcccattg cactttcaac taatgcttca
     4441 gcagttgcag agccatactt ttttaaaagt tctttctcta aagaacctcc attaactcca
     4501 attctaattt ttaaatttct ttccttgcat ttctcaacta ccatcttaac tctctctata
     4561 cttccaatat taccaggatt tattcttatt ccatccacac cttgttctat agcttcaagg
     4621 gctagcttat aatcgaaatg tatatctgca ataattggta tatttacact agactttatc
     4681 tttgctatat ttttagcagc tttaatatca ggaacagcaa ctctaactat atcacaacca
     4741 gcttcctcta atctctttat ttgagctatt gtagcatctg catctcttgt atcagtattt
     4801 gtcatagatt gtatacttat aggattttct cctcctattt ttacagttcc tacacttact
     4861 tctctcgtct ttcttctctc gtaactcata ttccacctct atatattatt ttatattgaa
     4921 tctctttaaa tattaaaata ttataaccta catgtatatt ataaattaaa aagctatcta
     4981 gttaaataag aataaatttc atatataaaa attaatttat tcttatttaa aactacatag
     5041 cctattttct taaaatctct aaaaactaaa tcattgaaat aatcttagta tatctttata
     5101 tgtgataaaa agcataaata acattaaagc tccaaaacca acaacattta tcatgccttc
     5161 tttatttggg tcgagttttt taccacctct aacagcttct agtaacagca taagtattct
     5221 ccatccatca agtgctggta ttggtaatag atttactata cctaaattta agcttatgac
     5281 agctgctaaa tacactacat ttatgatgcc tgtacgagca gcatctgata ccataccaat
     5341 aacacctact ggtcctgcta cagcattgcc aatccctcct ggtacacgtc ctgtaatcat
     5401 ttgacctaga aatgttagca tctgttttgt catatctaca gtagttttac atgcatttac
     5461 tattgacccc aaaaagtctt tttctctttg tggttgaatc cctatttcat actttccatt
     5521 gttattttta ggtgttacat ttacaacttt atcacttcca tctctagtta tactaagttt
     5581 taattctcca ccagaagaag tatttattat atttgcaact tcatcccaag tcttaacaga
     5641 attaccatta attttattta tcttatctcc tacttgaagc cctacagcct gtgcaggagt
     5701 atctggcatt atctttccta atttagtcgt tggagaacct atatacaaaa atactggaac
     5761 taaaagtatt gctgctaata ttatattgaa tattggtcct gctacaatag tactaaatct
     5821 ttgtaatata gacttgtttc caaaagaatt tggacttatt tgttcttcat cttcaccttc
     5881 catgctgaca tatccaccaa ttggaagtgc tcttatagaa tactctgtat ctttttttac
     5941 actatatatc ttaggcccca tacctataga aaactcattt actttgatac cacttctttt
     6001 tgcaaatata aaatgtccta actcatgtat taatacaatt atactaaata gtattaatgc
     6061 ggctattata gtcataaatt caccttccta tttttttatc agatttgcaa cataagctct
     6121 actccatcta tctgtttcta agatttcttc taatgttggt tcacttatgc tactatgaac
     6181 ttctaaagtc ttctctatgt aatatggtat gtcatagaaa ccaattttat cctctaaaaa
     6241 ttcacttact aaaacttcat ttgctgaatt taaaacagag gaataagttc ctcccatttt
     6301 taaacactca tatgccaatt ttaaacatgg aaaagtttct aaatctggtt cttcaaaagt
     6361 taaagtacta aattttgaaa aattcattct ttcaaagcta ctttccattc tatctggata
     6421 tgtaagagca tactgtattg gtagtctcat atctggacat cctaattgag caattattga
     6481 gctatctgta tactgcacca ttgaatgaat tatgctttgt gggtgaacta ctacatctat
     6541 attttcttgt tctactccaa ataaccatct agcttctatc acttcaagtc ctttattcat
     6601 aagcgttgaa gaatcaatac ttatttttct tcccatactc caatttggat gcttcaaagc
     6661 ttcattttta gttatatttg caagttctcc ctttttcttt cctctaaatg gaccaccaga
     6721 tgctgtaagt attatctttt ctatattttt tttattttct ccatttagac attgaaatat
     6781 agcactatgt tcactatcaa caggaagaat atttacattg tatttttctg cttctttcat
     6841 tacaagttca ccagcagtta ccaacgtttc tttattcgct aatgctatgt ctattccctt
     6901 ctttatagca caaagagttg ggactaatcc tatcatacct actacagcag ttaaaaggac
     6961 atccacttca tcaagtgatg atattatttt taatccttcc attcctgcta aaacttctat
     7021 ttctatattt ttaggcaaca tttcttttaa tttcaatgct ttattttcct caaaaactgt
     7081 tacatattta ggtttaaact ctactatctg ttctagcaat aattcaatat tactgttagc
     7141 tgatattgca actatctcaa acttatctct attatctcta actacatcca aagtttgttt
     7201 acctatagag cctgtagaac ctaaaattga tatctttttc atataataat cctttctaat
     7261 aataaatata ccctgtcaaa acattataac actactaaaa caattatttt tactattcat
     7321 agaaatttca tatattattc cacagattaa aattaatcta ttattttttg tattttgtgg
     7381 aatattataa aatattctag tattattaaa tgctcttttt tatttccaca acatcttcta
     7441 ttttacagtc tagtgctaaa catatcttct ccattgtatc catagaaaca gatttatcat
     7501 ttcctagtct gcctaaaaca gaaatactta tacctgatat atttacaaat tccattttct
     7561 ttatttttct gtcaattaac aatttccata atggagcgta tgaaatcatg ttatcacctc
     7621 gcaaaatata ttctatcaca ttttatctat tttggaaaat
//
LOCUS       DAGSRM010000134         7605 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.139, whole genome shotgun sequence.
ACCESSION   DAGSRM010000134 DAGSRM010000000
VERSION     DAGSRM010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7605
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.139"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(22..684)
                     /locus_tag="MJE33_003398"
                     /pseudo
     CDS             complement(22..684)
                     /locus_tag="MJE33_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440163.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
     gene            complement(844..1380)
                     /locus_tag="MJE33_003399"
     CDS             complement(844..1380)
                     /locus_tag="MJE33_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBY9649308.1"
                     /translation="MIGLKCGMVKLLEHQMIWDKSAKDVIILLKSIWDKTAIDIQHIG
                     STSIPSISAKPIIDIVVGVASLEEAKLYLERLEQCGIVFRGQDVPKQLLFAMGDFEKN
                     TRTHHIHVVEWNSVAWNNYINFRDYLNTFTDKAKLYDSHKQKLALQFEDNRKIYTQRK
                     QELINILLEEARLWRINT"
     gene            complement(1453..1866)
                     /locus_tag="MJE33_003400"
     CDS             complement(1453..1866)
                     /locus_tag="MJE33_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBY9649309.1"
                     /translation="MKMPKESIDTIMFAPCGMNCMVCYKHCYHKKPCAGCLNGDTGKP
                     EHCRKCKIKDCVCQKGLFYCFECFNFPCKLIKNLEKSYNKRYQASLMENSKFVQQYGL
                     EKFMQKQKERYTCSKCGGIISIHDRECSECQEKVK"
     gene            2359..2997
                     /locus_tag="MJE33_003401"
     CDS             2359..2997
                     /locus_tag="MJE33_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HBY9649310.1"
                     /translation="MDEKILELLQEMQGNISNMQGSISGIEGTISDMQSNINGIEGTI
                     SDMQSNINGIEGTISSMQGNINGIENTISGMQGTIGDMQGSISGMNGTISDMQGSISS
                     MDGTINSMQGSITDMQVEIKEVNSRLDNMEPDIKDLKTGQVETSSRLDTIQSDVTEIK
                     SDVKINRNTLDTVIKHTSSLVKDTSDIKNDLNNIEIVTSKNSNDIAKLKSVK"
     gene            complement(3605..3784)
                     /locus_tag="MJE33_003402"
     CDS             complement(3605..3784)
                     /locus_tag="MJE33_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBY9649311.1"
                     /translation="MLQTGKQGADKKYLDNMFFVKGYVEELLFLDKSYDIVFSCIVFH
                     HCTNVQNIFCIFWKW"
     gene            complement(4010..4543)
                     /locus_tag="MJE33_003403"
     CDS             complement(4010..4543)
                     /locus_tag="MJE33_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBY9649312.1"
                     /translation="MQYISYYHSPIGNILLASDNIGLTGLWFENQKYYAYNLDQEHQE
                     KELPIFEQTKKWLDIYFSGKEPNFTPSLHMVGTPFQTTVWKILQQIPYGKTITYGEIA
                     YKVAQQKGISKMSAQAIGGAVGHNGISIIVPCHRVVGTNGSLTGYAGGIDRKIKLLSL
                     EKVSVQHYFIPKKGSAL"
     gene            complement(6086..6658)
                     /locus_tag="MJE33_003404"
     CDS             complement(6086..6658)
                     /locus_tag="MJE33_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBY9649313.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGNCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
     gene            complement(6720..6983)
                     /locus_tag="MJE33_003405"
     CDS             complement(6720..6983)
                     /locus_tag="MJE33_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBY9649314.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDSYNRYCD
                     KFTQAVCDYESKYGALTNFGYESSEYPWSWISEPWPWDKSFYK"
     gene            complement(6983..7204)
                     /locus_tag="MJE33_003406"
     CDS             complement(6983..7204)
                     /locus_tag="MJE33_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBY9649315.1"
                     /translation="MGYLKEDKQDYCCEFARPYINRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYANPKNRVRAKKGGNMY"
BASE COUNT         2692 a         1264 c          903 g         2746 t
ORIGIN      
        1 agtgttcgta gctatttttt aatcaaactg gaatacagac tccattccta ggaaattggc
       61 tgaaatatag tctgctgtca aagatgctgt catcacagaa attaaaacag atactgaaaa
      121 gtttttatgg acttcttcaa gtgaaaacat aacacccgct agtggtgcat gaaaggctgc
      181 tgataatcca gcacttgccc cacaggttaa caggaatttt tcctctgtct tccctcggtc
      241 aagagctcta gaaatacctt tacctgccat agcaccaagc tgaatggaag gaccttccct
      301 tcccaaagca agaccaccca tgagtgataa gaatcctcca ataaattttg ttggcaacac
      361 tcgccaccat acttgtttta ttttccctgt catttccccc tcaagctgag ggataccact
      421 tccagaaatc attggctccc agtccaccaa ctttgataca atccacgcaa gtactgcaag
      481 tactgcaaac caagctatta tttttattgt agaaccttct acaaatttta aaatttggct
      541 aagccaagta ccagcctgcc ctaaaagtat cctgtataaa agcacaatca aaccagccac
      601 agccccaacc ataagtcctt caccaatcaa aattacctga aagtgctctg cccgtttgat
      661 aatatgagat gtatcctttt tcatactgtt cctccttcat ccaataattc tacacaatct
      721 gaatctccaa tgactaaagt catgagtgtg tacagatatt ttttaatcaa caaaaataga
      781 tttttattta ctaaatataa gaaaataatt gctcaatttc tcaataaatg tgaatttatt
      841 cttttaggta tttattctcc acagtcgtgc ttcttccagc aaaatattta ttagttcttg
      901 ctttctctgt gtatagattt tacgattgtc ctcaaactgt aatgctaatt tttgtttatg
      961 actatcatag agttttgctt tgtctgtaaa agtatttaga taatctcgaa aattgatata
     1021 attattccat gcaacactat tccattcaac aacatgaata tggtgagtac gagtattttt
     1081 ctcaaaatct cccatagcaa aaagtaattg ttttggtaca tcctgacctc gaaaaacgat
     1141 tccacattgc tctaaccttt cgaggtataa tttagcttcc tctaaagaag caacacctac
     1201 aacaatatca ataataggct ttgcgctaat actgggaata gaagtgcttc caatgtgttg
     1261 aatatcaatg gcagtcttat cccatatact tttcaataat ataatgacat cttttgcact
     1321 tttatcccat atcatctgat gttccaataa tttaaccata ccacatttta aacctatcac
     1381 tatataccac ctcaatataa tccaccttta tatatattac aattttttct atcttgataa
     1441 acagtgattt agctatttca ctttttcttg acactcgcta cattccctat catgaataga
     1501 tataattcct ccacatttag aacaagtata tctctccttc tgcttttgca taaacttctc
     1561 taaaccgtac tgctgaacaa atttactatt ttccataagg cttgcttgat atctcttgtt
     1621 ataacttttt tcaagatttt ttatgagttt acaaggaaaa ttgaaacatt caaaacaata
     1681 aaacaaccct ttttgacaga cacaatcctt tattttacac ttacggcagt gttctggctt
     1741 tcctgtatcg ccattcaaac aaccagcaca tggctttttg tgataacaat gcttatagca
     1801 aaccatacaa ttcattccac aaggagcaaa cataatcgta tcaatactct cttttggcat
     1861 tttcataaca ttttactcct ttcaaaaatc caatttgtcg aattgttata tattcatatt
     1921 gtaacataaa aaatagctaa aaaattcatg cactttcatt gccaataaat tttcaaggaa
     1981 atatgaaagt ttaaaactaa gaataaaaag agataaaaag gaagctacta atcaaaatat
     2041 tcaaatacaa gtaatcaata taaataactt aaggtatatt taaaagtata tgatattacc
     2101 agcagtttta aatacaaata taaccacaca tgcctcaaaa acacttatta cagtatctta
     2161 gaaacttaga caatacataa gattcattaa taaaaaagtt ctcaatatag tcattaataa
     2221 aaacgttctc aatatagata tatttaacca tgttttgaat agcagataat tatatgatta
     2281 aaaattaaat atgacatagc gtttgtggaa tataacacct aaattatgtt ataataataa
     2341 aacaggagtg tgatagtgat ggatgaaaaa atattagagt tattacagga aatgcaaggt
     2401 aatattagta atatgcaagg tagtatcagt ggtatcgaag gcactattag cgatatgcaa
     2461 agcaatatca atggtatcga aggcactatt agcgatatgc aaagcaatat caatggtatc
     2521 gaaggcacta ttagtagtat gcaaggcaat atcaatggta tcgaaaacac tattagtggc
     2581 atgcaaggca ctattggcga tatgcaaggt agtatcagtg gtatgaatgg cactattagc
     2641 gatatgcaag gcagtatcag tagtatggat ggcactatta atagtatgca aggcagtatt
     2701 actgatatgc aagttgaaat taaagaagtc aatagtaggt tagacaatat ggaacctgat
     2761 attaaagact taaaaactgg acaagttgaa actagtagta gacttgatac tattcagagt
     2821 gatgttacag aaattaagtc agatgtaaaa attaacagaa atacattaga tacagtgatt
     2881 aaacatacct cttcattagt aaaagataca tcagatatta aaaatgattt aaataatata
     2941 gagatagtca catctaagaa ttcgaatgat attgctaaat taaaatcagt taaataatat
     3001 actatagtaa aaaatacctg aaaatttcac atacttcttt aaagttatac caaattttat
     3061 acagaaatac tgattatccg acaacacact ttacttataa ataatttatt aaaaaaatac
     3121 tcattttatc ctaaaattaa cacgactaaa tgagtcttga tataataata aaaaataagg
     3181 gagattggac actaaaaagt tgtgtatctc ccttatattc tataatatag ttatataatt
     3241 tttcagccta aaataaaagt ccatctttga ttaaaacaca tccccttatc acttataaaa
     3301 ggataaaatc ttgttttcat ttttccattt atatcttctt gcttaagagt aataattttt
     3361 tcttttgata ctttaagtgt ttggttaaat ttaaccaatc gactaagttt tgttcaatat
     3421 ctatttttca tattttccaa tacttaatct attttttata aataattctt tcatttctaa
     3481 ctcacttaca ttttttacat aaaacgaaac atataaattt caaccttatc ttgtatggtt
     3541 cttaatactt aatgagtagc ttaagtatca atcattataa atttttccct tacatttaac
     3601 actcttacca tttccaaaat atacaaaata tattttgaac atttgtacaa tgatgaaata
     3661 caatacatga aaatacaata tcataactct tgtctaaaaa tagaagttct tcaacataac
     3721 ctttcacaaa aaacatatta tctaagtatt ttttgtctgc tccttgtttt cctgtttgta
     3781 gcatataaat tactgcatct aaacatataa ctactcacac gaaaaaagaa aataaacttc
     3841 caaaatacaa gctctaaaaa ctacctctaa agttgtataa tgtttactag gaataaggtt
     3901 gtataacata tagtttaaat aatcattttt taaaattgca agttattgct ttcaaaatta
     3961 gatgttttta tatcaaataa atgattaaga atttctaaat ttaatggctt tataatgcac
     4021 tccctttctt tgggataaaa taatgttgca cagatacttt ttctaaagat aacaatttta
     4081 tttttctatc aattccacca gcatacccag tcaaactccc atttgttcct acaacacgat
     4141 gacatggaac aataatagag attccattat gccctactgc accaccgatt gcttgtgctg
     4201 acattttaga tattcctttt tgttgagcaa ctttatatgc aatttctcca taagtgattg
     4261 ttttaccata tggtatttgt tgtaaaattt tccacactgt agtctgaaaa ggagtaccta
     4321 ccatatggag tgatggtgta aaattgggtt cttttcccga aaaataaata tctaaccatt
     4381 tctttgtctg ctcgaaaatt ggcaattctt tttcttgatg ttcttggtca agattataag
     4441 cataatactt ttgattctca aaccaaagac cagttaaccc aatattatct gatgctaaaa
     4501 gaatatttcc aattggacta tggtaatagc taatatattg catacaattc ctccttatat
     4561 tctgaaaact tcactcaaat cattagacac ttcttttcta tctaccatta tgtaaaaaca
     4621 tactaatata atttccttct aactacactt tcgttataat atctatcctc atatcatatt
     4681 agttttatat ttacctgcat ctgttcatcc ttttttccct ttccctttga tgaatataaa
     4741 attcatacat ttaagcttcc tactgtatta acttttatag aagtgtatat tataatttta
     4801 tcaccaaata gaccaaaaaa atatcgatat aacatcaagt aatatcataa tactttcaaa
     4861 tgggaaaagt aagaataggc tgtaaaaaag aaaagtattt ttatgttaga aatccagtaa
     4921 aatcgttagt agatagaata acagaaaacg aacaatttgc tatttatcat tttacacata
     4981 aatataattg gcgcaataaa gtgttttcta ttctacaacg attaataagg ttagaaaaaa
     5041 gtaaaaatga actgtatatg tatgaagcat tagtttctgt ttctttcctt tggcttatta
     5101 tgataaaaaa tattgttttg ccaccaataa aacacaaaaa tataattaat atccatatgc
     5161 aaaaaatttt acgttatatt gaaaaatact attgttttac attatattga agggtactat
     5221 tgtgaaaact taattttgga gtactatcaa gcagtggtaa tatcaataaa tcagagtgct
     5281 ccagatgatt tgaactaagt ttaaatacta taccttataa atattttact gagttttgtt
     5341 tattaaaagc aactcaattt ctaaaaggca caaatgagct aataagtgac attgctataa
     5401 aagttgtttt tcatcaaata atctatttta gtaaatgttt taaagaaaaa acgggatatt
     5461 ctccaaaagt gtacagaaat ataaaaaaag agatatgatt ttttatttct tttgattatc
     5521 caaagagtaa ataaaaaata ttgtaaaaaa cttactaaca acggcaatgc tttgaactat
     5581 ggtaatactt tgaacttttg attggacttt ttttgctaaa ataatatatt taaatagcaa
     5641 agtaatctgt ttaatcaata aaaaataaga gataaaagaa atcaactaaa acttttacct
     5701 cttattttga tagtttatca gagctaatat ttacactctt tttatttttg atacttcatt
     5761 aaaaatgatg cttattctct tttcattttt aatacttcat tagaacttat gcttattctc
     5821 ttttcacttt taattcttca ttagaactta tgcttactct ccattcactt ttaattcttc
     5881 attaggacta tgcttattct ccattcactt ttaattcttc attaaaaatg atgcttattc
     5941 tccattcact tttaattctt cattaaaaat gatgcttatt ctccattcac ttttgattct
     6001 tcattaaaaa tgatgcttat tctctgttca cttttaattc tttattaaaa ctgatgctta
     6061 ttctccattc actttcaatt cttcattaaa actgatgctt gcactctgta aactcataaa
     6121 catccattaa tgcttcacca aatctttgat agtgaactac ttccctctca cccaaaaatc
     6181 tcaatatatc tttcaaatca gggtcatcag ttaaatttat taattgatag tacgtagcca
     6241 atgctttttg ttcagcagcc atatcctcat gtaaatcagt taccgaatcg ccaaagacat
     6301 taatataact tgtagtaaag tttactccat tagaatctac tggctgaaga ccatgtccaa
     6361 aattagcata attactacca agtccagcag ccttaagttc ttctggagta caattgccta
     6421 ttaattgata taacactgaa gaaattatct caacatgagc catttcctct gtacctatat
     6481 cagttaaaag tgcacgagat tttccagttg gcatagtata tctttgttga agatatctag
     6541 ttgcagctgc caactcacca ttaggtcctc ccaactgagt catgatatat tttgccattc
     6601 tagggtctgc ttgtcttatg ttaactggat gttccagtgt tttttgataa atccacatat
     6661 tatcttactc ctccttaata tatatttaat aataaatcta attttaaagt ttaataattt
     6721 tatttataaa atgatttgtc ccatggccaa ggttcagata tccatgacca aggatattca
     6781 cttgattcat atccaaaatt agttaatgct ccatatttag attcataatc acacacagct
     6841 tgtgtaaatt tatcgcagta tctgttgtat gaatcaagag ctttcttatc ccttggattg
     6901 ttatctaaat ataaatttaa ttctatacag gcaaattgat attctgaaat tctctctaaa
     6961 agttctcttc tatttaatga cattagtaca tgttaccccc ttttttagct ctaactctat
     7021 ttttaggatt tgcatatagc caatcattat agttatttga ctctattaaa tctaattcag
     7081 gaaataaagt acctcttttt aatgctgttt ttggattgta tgtgtcatca taaatttgac
     7141 gattgatgta tggtctagca aattcacaac aatagtcttg tttatcctct tttaaatatc
     7201 ccattaatta tcctccttaa tttttagaat tttaaaatag cattattttt aaaaattgta
     7261 tactggttct atattataac tggtagtttt aataggtctt taataagatt aaatgattaa
     7321 ataagtatct ttttttctta ttttacctct aaaaattttt ctaattagtc tttcataaat
     7381 atctatttta aataaataat atagagttta tacaattttt tatagcttaa ttactattta
     7441 ctatatatta ttcattttta aataaaatgt gattattttt tactaaaaac gatataaata
     7501 tacaatttaa taacattttt aacaaataaa tttatattga aatacattaa attatataat
     7561 tttatttatt ctgagagtat aattataaaa aaaagaatag gatat
//
LOCUS       DAGSRM010000135         7271 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.140, whole genome shotgun sequence.
ACCESSION   DAGSRM010000135 DAGSRM010000000
VERSION     DAGSRM010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7271
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.140"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            167..541
                     /locus_tag="MJE33_003407"
     CDS             167..541
                     /locus_tag="MJE33_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY9649316.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            538..1401
                     /locus_tag="MJE33_003408"
     CDS             538..1401
                     /locus_tag="MJE33_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9649317.1"
                     /translation="MISMDAITIKNLNKTYKDFSLQDISFSVPKGSVMGFVGENGAGK
                     TTTLKAILNLISYDSGNIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIM
                     SKIYKNWNDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHKPKLLILDEATS
                     SLDPIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYITFIHKGKIVFSENKDE
                     LIDTMGVLKCKPSDFDNLSREDYSYYRKSQFGYEVLLKDKHRFISRHPNCIVDNTSIE
                     EIMLFYVRGDK"
     gene            1401..2039
                     /locus_tag="MJE33_003409"
     CDS             1401..2039
                     /locus_tag="MJE33_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBY9649318.1"
                     /translation="MKGLILKDLLNLKGNIKFILLFIIMFGFMSSLGDGNVNNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPINRNDIVLSKYLTMLIFSFIGVLVSLIVSV
                     TIGYFKNTLILNETLLINALILSISVCFGSLILPLIYKFGTERARLLMILCFLVPTLA
                     LLVFKSILENISSPISIEIILNTLVYSLPFVAILLFVISYFISSKIYSKKEV"
     gene            2531..2944
                     /locus_tag="MJE33_003410"
     CDS             2531..2944
                     /locus_tag="MJE33_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY9649319.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKAEKIAKERGCKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLENYPLTGKRYYYRKIL"
     gene            3135..4352
                     /locus_tag="MJE33_003411"
     CDS             3135..4352
                     /locus_tag="MJE33_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HBY9649320.1"
                     /translation="MLGKKCMDYLQTLGKISSTTNGLTRLILTQEHKKSIDLISSWME
                     GLNLDIEIDDIGNVIGTYKSSFPNAPTLVVASHQDSVKCGGIFDGMLGIIVPLVGLEE
                     AKHNNRSYPFNIKLIAFAEEEGTRFETSLMGSKVFAGTFKEELLKSVDENGITLEEAV
                     TKFGFNTKNLTNLHPRKDVDAYLEFHIEQGPVLENESLPAGIVSSITGFKSFKISVNG
                     KSGHAGTLPMNMRLDAGCCACECVLAIEKVAKTTTDLVATVGKMNFYPSSSNVVPERA
                     EFTLDVRSCSQEILDNSVEKIFNEISHICENRKLNYTSELAFENVPVPCSNKITKIIE
                     KSFIDLNLNPFYIYSGAGHDAQEMDNITDIGMVFIRCAGGVSHNPNESVSVDDLDTAV
                     KIFLKILDNLDLK"
     gene            4514..5212
                     /locus_tag="MJE33_003412"
     CDS             4514..5212
                     /locus_tag="MJE33_003412"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBY9649321.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSLPTPFYIDRPINHELMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKESINI
                     PILNIVEETVKKLPTISQKVTLFSTSSTFESTIYQNGIIHNGHEFIFKDEWQIKLNNL
                     IQNIKMDKENPHNIDIWNKLIEDVKNESIENIVIACTDLNVVLEKSYPSINIVDSSKC
                     LAESVVNKYLKLVK"
     gene            complement(5432..6049)
                     /locus_tag="MJE33_003413"
     CDS             complement(5432..6049)
                     /locus_tag="MJE33_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HBY9649322.1"
                     /translation="MKKIILALTTFLLAISLTACSSAKPEDTIESFFNSAKKFDFEGM
                     NKVMENNDEKYKDILKELDTKDPNAQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMIGMSFSGQDLTDEKTTEMLVSIMKEKQKSVKETYVTK
                     NVEFECSKKDNKWIISSVNDAVADVLLSNLVTAGQEFSNSMNTLE"
     gene            complement(6176..7198)
                     /gene="argF"
                     /locus_tag="MJE33_003414"
     CDS             complement(6176..7198)
                     /gene="argF"
                     /locus_tag="MJE33_003414"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBY9649323.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAHVTYLGPNDCHMGKKESVSDTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTVIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGDIMLTE
                     NIDDVKGADVIYTDVWVSMGEEEQYEARINQLKDYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQEIYEKFGLSELEVTNEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLV"
BASE COUNT         2652 a          918 c          958 g         2743 t
ORIGIN      
        1 tttaaaaaaa tttatatcta tttaatatgg cagactttca taaaatacat tacatttatt
       61 aaatgtataa tattggcaat tagttttttc taaaatcagt tgacatatta tatatatatt
      121 tatataatgt gtatatacta tatatacaat ttaaggaggt gtcttattga atatagttat
      181 tagtaattcg agtgggaagc caatatatga acagattact acacaaatta aaagtatgat
      241 tataagtgga gagttaccag aaggctcagc tcttccaagt atgagactat tggcaaaaga
      301 acttagaata agtgtaatta ctacaaaacg agcatatagc gatttagaac gagacggatt
      361 tattgagaca gttactggta aaggcagttt tgtatctagt aaaaacatgg attttgtcaa
      421 agaagaacaa tttcgtttaa ttgaagaata cctacaaaaa gcagttgata cagcaaaaag
      481 tagtgatatt acatatgacg aattaaaaga tattttattt ttattatata aaggagaatg
      541 ataagtatgg atgcaataac tataaaaaat ttaaataaaa cttataaaga ttttagccta
      601 caagatatat ctttttcagt accaaaaggg agtgtaatgg gatttgtagg tgaaaatggt
      661 gcaggaaaaa caactacatt aaaagcaatt ttaaatttaa tatcttatga tagtggaaat
      721 atagaaatct ttggtcttga tagtaaaaaa aatgaaaaag aaattaaaga gcaaattggg
      781 gttgtatttg agggaagcaa ctttcatgaa aatttaaaca ctgaccatgt ttcaaaaatc
      841 atgagtaaaa tctataaaaa ttggaatgac acattattta aagactactt aaaaaaattg
      901 agagtaccag acaataaatt aattaaggag ttttcaaagg ggaataagat gaaacttagt
      961 attgctgtag ccttatctca taaaccaaaa ttattaatac tagatgaggc aactagttca
     1021 cttgacccta tcgttcgtga agaaatacta gatatatttt tagattttat acaagatgaa
     1081 gaacactcta ttatcttatc ttcacatatt acaagtgatt tagataagat agcagactat
     1141 attacattta tacataaagg aaaaattgta tttagtgaaa ataaggatga attaattgat
     1201 actatgggag ttttaaaatg taaacctagt gattttgata acctttcaag agaggattat
     1261 tcatattatc gtaaaagcca atttggatat gaagtacttc ttaaagataa gcatagattt
     1321 atctcaagac atccaaactg tattgttgat aatacgtcta tagaagaaat tatgttattt
     1381 tatgtgagag gtgataaata atgaaaggtc taattttaaa agatttactt aatttgaaag
     1441 gaaatataaa gtttatactt ttattcatca ttatgtttgg ctttatgtct tctttaggag
     1501 atggaaatgt taataacttt ataggtgtta ttatagtttt atgtactaca atgattgttt
     1561 caacattttc atatgatgat ttgaataaat gggatagtta tgtacttaca atgcctatta
     1621 atcgcaatga tattgtatta agtaaatatt taactatgtt aatatttagc tttataggtg
     1681 ttcttgtttc acttattgtt agtgtaacta ttggttattt taaaaataca cttatattaa
     1741 atgaaacact acttattaat gcactaatct tatcaatttc agtatgtttt ggtagcctta
     1801 ttctaccact tatttataaa tttggtacag aaagagctcg tttattaatg attttatgct
     1861 ttttagttcc aacactagct ttattagttt ttaaaagtat tttagaaaat attagttcac
     1921 ctatttctat agaaattata cttaatacac tagtatattc tcttccattt gttgctattt
     1981 tactatttgt gatttcatat tttatatcat caaaaatcta tagtaaaaaa gaagtataaa
     2041 atatactata tatgtttctg tatttaagta tttaaataaa atactatcga gtttattttg
     2101 gtagtatttt attttttaat tattaatctc tactaggtat ttattatttt attacttata
     2161 tttaataaga ctatttacta ctttctaatc ttatttatat aatttattca ttttataaat
     2221 tgaactacta atgatattat tgaacatagc aatagaataa catctaaaat agtttttatg
     2281 tattaaatta acttatttta atattcaaca atttaggttt actaaattta ttgatgattt
     2341 ctttttatat aacttaaact acatttttat ttaaaaaaat ttaatatcaa caatataaaa
     2401 ttaagaataa gtattattag tttgacatta gcataaatgc ctattagaat aatgttaaac
     2461 tatattcttt ctactgattt tattaaaaaa taccaatata caaaagatta tacaaggaga
     2521 aagttcaaat atggaattag aaattacaga aaaaatagaa aaacaagatg aagatactat
     2581 ttttcaagga ttattaaaat ataatcttgc tagacttgaa gataaaaacc cagtggattt
     2641 aggaatatat attcgagata aagcaggtaa aattgttgca ggttttatag gtgttactca
     2701 tggtaattgg ctttctataa aatacttatg ggttagtgag aagcttagat ataaaggtac
     2761 tggaagtcaa cttttatata aggctgaaaa aattgctaaa gaacgaggtt gtaaatatgt
     2821 ttttttagat acattcagtt ttcaagcacc taaattttat gagaaatttg gataccataa
     2881 agtattttca cttgaaaatt atcctctaac tggaaagaga tattattata gaaaaatact
     2941 ataataataa aaatcatatt tcatattttt aaattaaaac ttctgctctt cgtctacaga
     3001 ctttatgttt ttatatcatt attaatacta aaatagtatt ttactataca tacaaaatta
     3061 tttgaaagtt caaaaattat aagtataaaa gctaatctta gcttaaataa aaattatatt
     3121 tttaggaggt aaatatgtta ggaaaaaaat gtatggatta tctacaaaca ttaggaaaaa
     3181 taagttcaac aacaaatggt cttacaagac ttatcttgac tcaagaacat aaaaaatcta
     3241 ttgatttaat tagttcatgg atggaaggtt taaatcttga tattgaaatt gatgacattg
     3301 gaaacgttat aggtacatat aaatcaagct ttccaaatgc tcccacatta gtagttgcat
     3361 ctcatcaaga tagtgtaaag tgtggaggta tatttgatgg tatgttaggt ataatagtgc
     3421 ctctagttgg tcttgaagaa gcaaaacaca ataataggtc ttatcctttc aatattaagc
     3481 taattgcttt cgctgaagag gaaggaacta gatttgaaac ttctttaatg ggaagtaaag
     3541 tttttgctgg tacttttaaa gaggaacttc ttaaatctgt tgatgaaaat ggaattacac
     3601 ttgaagaagc tgtaacaaag tttggattta atacaaaaaa cttaacaaat ttgcatccta
     3661 gaaaagatgt agatgcatat ttagaatttc atatcgagca gggtcctgtt ttagaaaatg
     3721 aatctcttcc tgctggaatt gtatcaagta ttacaggatt taaaagtttt aagatatcag
     3781 taaatggtaa gtctggacat gctggtacac ttccaatgaa tatgagactt gatgctggat
     3841 gttgtgcctg tgaatgtgta ttagctatcg aaaaagtagc taaaacgact actgatttgg
     3901 ttgctactgt aggaaagatg aatttctatc catcttcatc aaatgtggta cctgaaagag
     3961 ctgaatttac tcttgatgta cgctcttgtt cacaagaaat tcttgataat tctgtagaaa
     4021 agatatttaa tgaaatatca catatatgtg aaaatagaaa acttaattat acaagtgaac
     4081 ttgcttttga aaatgttcct gtaccatgtt ctaataaaat aacaaaaatc atagaaaaaa
     4141 gttttataga tttaaatctt aatccttttt atatttatag tggtgctggc catgatgctc
     4201 aggaaatgga taatataact gatattggta tggtatttat aagatgcgct gggggcgtaa
     4261 gtcataatcc taatgaaagt gtttctgtag atgacttgga tactgctgta aaaatatttt
     4321 taaaaatact agataatctt gatttaaaat aattcttata taagaaagta tattaaaata
     4381 tataatctaa aaaatcactt tatatttata ttatataagt gtacaatata atctattata
     4441 aaatataatt ttatgctttt atagattttt attttataca taaatatatt tatatataaa
     4501 ggagataatt tttatgaatg aaaaattaat tggtattttg gctggaatgg gtccaaggtc
     4561 aacagcccct tttgttgatt taattattga tgagtgtcaa tctcaatatg gagcaaaata
     4621 tgatgatgaa tttccaaaga tgatgatata ttcattacct accccatttt atattgaccg
     4681 tccaataaat catgaactta tgaaagaaac tataattgat ggattacaaa agcttgaatc
     4741 aactggtgta aattttattg caatgccatg caattcagca catatatact ttaaagaatt
     4801 aaaagagtct attaatatac caatattaaa tattgtagaa gaaacagtta aaaaattacc
     4861 tactatctca caaaaagtaa ctcttttttc gactagttca acttttgagt ctacaattta
     4921 ccagaatggt ataatacata atggacatga atttatattt aaagatgaat ggcaaataaa
     4981 attaaacaat cttattcaaa atataaaaat ggataaagaa aatccacaca atattgatat
     5041 ttggaataaa cttatcgaag atgttaaaaa tgaatctata gaaaatatag taattgcttg
     5101 tactgattta aatgtagttt tagaaaaatc atatccttca ataaatattg ttgattcatc
     5161 aaaatgcttg gctgaatcag ttgtcaataa atatctaaaa ttagttaaat aaatttttca
     5221 tgcactatat tataaaaaat atatgttata agtttatctt aaatagtata ggcttataac
     5281 atatatcata gtttcttgac aatatagaaa ttaaccatgt atttcattat attaataaaa
     5341 aggataccaa aaatggtatc cttcataaat atttatatgt ttatttataa aaataatcat
     5401 atatttatta ctttactaca cctaaacaat attattcaag tgtattcata gagttagaaa
     5461 attcttgtcc tgctgtaaca agattagata acaatacatc agctactgca tcatttactg
     5521 aacttattat ccatttatta tcctttttag aacattcaaa ctctacattt tttgtaacat
     5581 aagtctcttt aacgcttttt tgtttttctt tcataattga tactaacatt tcagttgttt
     5641 tctcatcagt taagtcttgt cctgaaaaac tcataccaat catctttgta aatgcctcag
     5701 ctactatttc ttgaagtaat ggtgttgaat ctataaattt acattctact ttgattgtag
     5761 ccttatcacc tttaacctcg ctgtctttta ttgtatatgt gattttagat gcattctgtt
     5821 ttagataatc taaaacatat tgggcatttg ggtcttttgt atctaattcc tttaatatat
     5881 ccttatattt ttcgtcatta ttttccatta ccttattcat gccttcaaag tcaaactttt
     5941 tcgcactgtt aaaaaaactc tcaattgtat cttctggttt tgcacttgaa catgctgtta
     6001 aagatattgc taataaaaaa gttgttaaag ctaatatgat ttttttcata tctcaaatcc
     6061 tccctcttaa tttgttgata cataagttat tttaacatat aatgagagtt tttgaataat
     6121 atatttaaaa tattttataa attttcatta aagtaaagtc tatattaaaa catatctata
     6181 ctaaattatt gttcatattt acatcggtca tagtagaaac cataacagct tttattgtat
     6241 gcattctatt ttcagcttct tcaaatacct ttgaatattt tgaattaaat acctcgtttg
     6301 taacctctaa ttctgataat ccgaactttt cataaatctc ctgagcaacc ttagtttcta
     6361 aatcatgaaa tgctggaaga cagtgtaaaa atataacatt ttcattttca gttttcttta
     6421 tcatatccat atttacttga taatctttaa gttgatttat tcttgcttca tattgctcct
     6481 cttctcccat agaaacccat acatctgtat atataacatc tgcaccttta acatcatcaa
     6541 tattttcagt caacattata tctcctttgg atttcttggc tatttccttc atttccttaa
     6601 ccaattcttc atttggccaa agttccttag gtgctagtgc tacgaaatgt attcccattt
     6661 tagcacatcc tatcatcagt gagtttccca tattatttct agcatcacca acatatacaa
     6721 atttaacttt attcaaatct ttatctacat tttcaattac tgttaaaaaa tctgccagta
     6781 tttgtgttgg atgatattca tctgtaagtc cattccaaac aggtacacca gaatattttg
     6841 ctagactttc aactgtttct tgtttaaagc ctctaaattc tattccatca tagaatcttc
     6901 ctagaacttt tgctgtatct gatacagact cttttttacc catatgacaa tcatttggac
     6961 ctagataagt tacatgtgct ccttcatcat gagctgctac ttcaaatgaa catctagttc
     7021 ttgttgatgt tttctcaaat aataaagcta tattttttcc ttgtagcaaa tcgcctttta
     7081 taccaacttt tttcttagtt tttaaataac ttgataaagt taataaatat ctaatctctt
     7141 cttttgtaaa atcctttaat gtcaaaaaac tcttaccttt taaacttata ttattcatat
     7201 taattcgccc cttcttataa actttttaaa ttcaattaaa cttgttagca ctttactttt
     7261 ttatttactt c
//
LOCUS       DAGSRM010000136         6985 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.142, whole genome shotgun sequence.
ACCESSION   DAGSRM010000136 DAGSRM010000000
VERSION     DAGSRM010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6985
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.142"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            100..774
                     /gene="fsa"
                     /locus_tag="MJE33_003415"
     CDS             100..774
                     /gene="fsa"
                     /locus_tag="MJE33_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBY9649324.1"
                     /translation="MQLILDTGNVEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEEILEEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GIKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHGVHSVTVPYDICKSMMNHPLTDWSVDKFIEDWENTFGKG
                     SKTNNI"
     gene            complement(896..2290)
                     /locus_tag="MJE33_003416"
     CDS             complement(896..2290)
                     /locus_tag="MJE33_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HBY9649325.1"
                     /translation="MGIILKPKQFYMTVIATIILYFSLNYSFNIKFSICLSFLTILIW
                     AVDSVEKTFVALFFVILAFIFNIAPIKVILQFLFTENFYIIILAYIITNAVTKTGVAK
                     IISEKLILNTVNTPKKMILLSYILGILLIFFIPQPFPRVILVSAFYKEFLKEQQITED
                     SKSILLFSIFTASTFTSMFFVNGDTLLNYVVLELGNCNINWGQWAFYMSVPTIITCFI
                     TYFLFIFVFKKELHSIKFISNENLEGNKLHFSYSNLKKEFNLLNKEQKHVFLCLGIMF
                     FMFLTQFIHNINTLIIMSICVLLLLLRKIVGFSTLKEINWKVLIFFIAAFSIGGVLKY
                     SGVVDIMGNYLIKFIPNSSEIISILFLITLTIILNICLGSAVTTSSVVIPLLGSLHIL
                     KENSVTLCLFVYIIVSIQYILPFHHATIMVGHGENLYNSKVIFKYGLTLIPLTYFIII
                     CITFPWWKFIGLEI"
     gene            complement(2368..3714)
                     /locus_tag="MJE33_003417"
     CDS             complement(2368..3714)
                     /locus_tag="MJE33_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBY9649326.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLKLMNTPESVFAQSKLYLTIIFSGILFSAGYNSVSAILRGLGDS
                     VTPLYFLIIATILNIVLDLTFIVVLRMGVEGVALATIMAQAVSFIISIIYLNKKHEVL
                     KFKIKGIVYDNKIFKDGLRLGLPSGVQQMLFSIGNMALQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVTVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSKFFGTDGIWMGIPFGWTLGLIVTVIYYKKGHWKTKAIVNHRINE"
     regulatory      3964..4066
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            4160..5536
                     /locus_tag="MJE33_003418"
     CDS             4160..5536
                     /locus_tag="MJE33_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBY9649327.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEIIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCVGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFI
                     NPEYAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPTNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSVVMIVLAIL
                     FIIKLWLL"
     gene            complement(5650..6795)
                     /locus_tag="MJE33_003419"
     CDS             complement(5650..6795)
                     /locus_tag="MJE33_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBY9649328.1"
                     /translation="MNTFEQLKISSTLIDGLKKQDITSPTEVQSLVIGNIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAELSVT
                     SLALIGEVNIQKQIKNIKAVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIVLDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKALAATNPKKAIVFVNNEKNIEVIVSKLNYHNYKAIGIFGN
                     MEKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     ARGNRSGNTISIITKKELDIIKDLQKEFNIVITPKTLQNGELIDIIK"
BASE COUNT         2516 a          932 c         1014 g         2523 t
ORIGIN      
        1 aaacaataat aaaaaataaa aaacaataag caaaatgttg aaaataatac caaatcaata
       61 tataataaga ataaagaaag attaaagggg gatattttaa tgcaactaat cttagataca
      121 ggtaatgtgg aggagattaa ggagttatgt acttgtttac caatagatgg tgtaactaca
      181 aatccaagta ttgtttcaaa agaaaaaaag aattttaagc aacttataaa tgaaattggt
      241 gagattatag gtgaggatat gcctatacat gctcaagtgt taagtacaaa gtatgaagaa
      301 atattagaag aagcattata tataagtagt ttgagaaaaa atatatatgt aaaaatacct
      361 gtaacacaag atggtttaag agctataaaa gaccttcaca gaaaaggtat aaaaataact
      421 gctactgcaa tattcactgc tcatcaaggt tttttagctg caaaggctgg agcaaattat
      481 attgcacctt atgtaaatag gcttgataat atatctggtg atggagtagc tatggtttca
      541 gaacttataa aaataataga tacttataaa atggatacaa aagtacttgc agccagtttt
      601 aaaaatgcac aacaagttat agaattaatg caacatgggg tgcatagtgt tacagtacca
      661 tatgatattt gtaaatctat gatgaatcat ccattaactg attggagtgt agataagttt
      721 atagaggatt gggagaatac ttttggaaaa ggaagtaaaa caaataatat ttagtattat
      781 ggtaaaagag tagttttgtc caatttcaca agaacttata taaaaagatg aaaatatgaa
      841 ttttcttttt aataatttcg ttattatctt aagttaattc atattttcat cttttttaga
      901 tttctagtcc tataaatttc caccaaggga atgttataca tattattata aagtaggtta
      961 aaggaatcaa agttaaaccg tatttgaaaa taaccttgct attatacaga ttttcaccat
     1021 gaccaaccat tattgtagca tgatgaaatg gtaatatgta ctgtatgctt actataatgt
     1081 agacaaatag acataaagta acactatttt cctttagtat atgtaaactt ccaagtaacg
     1141 gtattactac agatgatgta gtgacagcac ttccaagaca tatattcaag attattgtga
     1201 gagttatcaa aaacaatatt gaaataattt ctgaagagtt tggtataaac tttatcaaat
     1261 aatttcccat tatatctaca actccagagt actttaacac tcctccaatt gaaaatgctg
     1321 ctataaaaaa aataaggacc ttccaattta tttcttttaa agtactaaat ccaactattt
     1381 ttcttagtaa taaaagaagt acacaaattg acattataat taatgtgttt atgttatgta
     1441 tgaactgagt taaaaacata aaaaacatta ttcctaggca taaaaatacg tgtttttgct
     1501 ctttgtttag caaattaaat tcttttttta aattagaata tgaaaaatga agtttattac
     1561 cctcaagatt ttcattagaa ataaatttta ttgaatgcaa ttctttttta aagacaaaga
     1621 taaataaaaa ataagttata aaacaagtaa ttatagttgg aacagacata taaaaagccc
     1681 attgacccca atttatatta caatttccaa gttctagtac aacataattt agaagagtgt
     1741 ctccatttac aaaaaacatt gatgtaaagg tagatgctgt aaatatacta aataaaagta
     1801 tagattttga atcttcagtt atctgttgtt cttttaaaaa ctccttgtaa aaagcagata
     1861 caagaataac ccttggaaat ggttgtggga taaaaaatat taaaagtata cctaaaatat
     1921 acgataaaag aatcattttt ttaggggtat ttacagtatt taaaattaac ttctcagaaa
     1981 taatttttgc gacacctgtt tttgtaactg cattagtaat tatgtatgca agaattatta
     2041 tgtaaaaatt ttctgtaaat aaaaactgta aaataacctt tattggtgca atgttaaata
     2101 taaacgctag aattacaaaa aacaatgcaa caaaagtttt ttcaactgag tctacagccc
     2161 atatcaagat agttaaaaaa gataagcata tagaaaattt tatgttgaaa ctgtaattaa
     2221 gagagaagta gagaattatg gtagctataa cagtcatata aaattgtttt ggcttaagaa
     2281 taatacccat ttttatattc acctccataa tttattatat cataatggag gtgaatacaa
     2341 tgaatgtgaa tacattttat aaaaagatta ttcattgatt ctatgattta caatagcttt
     2401 agttttccaa tgtcctttct tatagtagat aactgtaaca ataagaccta aagtccaacc
     2461 aaaaggaata cccatccaaa ttccgtctgt tccaaaaaat ttagaaagca tataagaagc
     2521 tggaagtctt ccaagccata aagatactat tgagcttata agtgcaaaca tggaatcacc
     2581 agcacctttc attgcacttg aaagtacaaa agaagttcca ataaataaga agaatggtcc
     2641 tattataaat aagtaagaag agccaatttt aatgacatct ttatcagtat tgaatagagc
     2701 aattagattt tccctaaata gcaaaattaa agcaataact gtaactgcta aaactaaggt
     2761 catttttata gattctcgaa tgcctttttt aactctttca ttttctccag cacctatgtt
     2821 ttgagcaaca aaagtagaaa cagcagaacc aagattcata ataggtaagc ttataaaatt
     2881 ttctatccta agaccagcac caaaagctgc catagcagat gtaccataac tattaactaa
     2941 aaattgaaga gccatatttc caattgaaaa caacatttgt tgaactccac ttggaagtcc
     3001 taatctaagt ccatctttaa atattttgtt atcatataca attcccttga ttttaaattt
     3061 aagcacttca tgttttttat ttaaataaat tatacttatt ataaatgata ctgcctgagc
     3121 cattatagtg gcaagtgcaa cgccctcaac acccattctt agcactacaa taaaagttaa
     3181 gtctaaaact atatttagaa ttgtggctat tatcagaaaa taaagtggag ttactgaatc
     3241 tccaagtcct ctaagtattg cacttactga attatatcca gcagaaaata atattccact
     3301 aaatattata gtaagataca attttgattg agcaaaaacg gattctggtg tattcataag
     3361 cttaagcatt gggccaccaa atactattcc aagaatactt attaatatag aaccaataaa
     3421 taaaagtata tatgtagtat caatagtagc ttttaccttt tttaaatctc ctgagccata
     3481 aaattgtgac actaaaatag aaaatccaag gctaacaccc ataagcgctg ccaccaataa
     3541 aaacattata gggttagctc cagagactgc cgccattgct tcttttccaa taaatctacc
     3601 aactattata ctgtcagctg tattgtatag ttgttgaaat aaactaccaa taagcatagg
     3661 catggcaaaa aagaatatgg actttccctc atgacctgta gttaaatctt tcacattatt
     3721 cctccaaata aaaaaattat taatttatta tagtataatc ttaacataaa ctttatatcc
     3781 aaactataaa taaattgtat caaaatatca tatatttttt taaaatgtaa agaatataat
     3841 tataatttga atttaataaa tcaatattta atataatcga atattaatac aaaatcaata
     3901 caatatatac gtatatattg acaaaattaa ttatataaat taaaataata ttcgtaaaga
     3961 aaaattcatg ttttgtactg tataacatat aattttgaca atatgggtca taagtttcta
     4021 ccggaatacc gtaaatattc tgactatgta tactattatt tagttaatta aatatttaat
     4081 taactgaatt atgaaggata cggctcagct tatcaactac tgtgtcgttt ttttgtaaaa
     4141 tttaatagga ggtgttgtta tgcaaacaac aaacagtagt actatgcaga aactttttcc
     4201 aattttgact aataaaaatg tcgacatgaa gaaggaaata attgcaggag taactacttt
     4261 tttaactatg gcttatataa tagctgtaaa tccaaatatt ttatctgaga caggcatgcc
     4321 tgcaggtgca cttgttacag gtacttgttt agcagcagca tttggatgta ttttaatggg
     4381 tgtagttgct aaccttcctt ttgctttagc atcaggtatg ggtcttaatg cattttttgc
     4441 atatacagta gttttacaaa tgggtgttcc ttgggaagta gctcttacag cagtatttgt
     4501 agaaggtatt atttttatag tattatcagt aagtggagtt cgtgaggctg ttgtaaatgc
     4561 gataccaaaa aatatgaaat tagctgttac aggtggtata ggaatattta ttgcacttat
     4621 aggtctagtt aacagtggaa ttgttattgg agaccaagcc actcttatca aaatgggtag
     4681 atttacacca gcagtaataa taacttgtgt aggattaata attattgctg tattagataa
     4741 aaaaagagtt aaaggttcta tactttttgg aatagtagtg agttcattac ttgcttgggc
     4801 atttgctttt ataaatccag agtatgcaca aaaattaggt atatatttac ctggtggatt
     4861 atttaaattc gaaagtttag caccaatagc aggtaaaata gatttaggat atgtactaca
     4921 tccaacaaat ataggtggtt ttttagtagt agtttgtaca tttttatttg ttgatttttt
     4981 tgatacagta ggtacattag ttggtgtttg ttctaaagca aacatgttag atgaaaaagg
     5041 gaatgttcct aatgtaggta gagccttact tacagatgcg gttgcaacta cagttggtgc
     5101 aggacttgga gtatctacag ttacaactta tgttgagagt tcaacaggag ttatcgcagg
     5161 tggaagaact ggatggacag caataacagt tggaatttta ttcttagctg caatgttttt
     5221 ctctcctgta tttatagcaa ttccatcatg tgctacagca ccagctttaa tatatgttgg
     5281 atatttaatg cttggtacag ttaaggatat agagtttgat aatataactg aaggtgttcc
     5341 agcttttgtt actattgctt gtatggcatt aacatatagt ataggtgatg gtttaacttt
     5401 aggtatatta acttatgttt tcgtaaatat attttataat atatttggag ctaaaaaggc
     5461 agaagacaaa aaacatgttt ctgtagtaat gattgttctg gcaatattat ttataataaa
     5521 actttggcta ctataatagg tattatttaa aattttaatt taatataaaa taaagggctg
     5581 taatttatta tcaattgtga tgataaacta cagctctttt gtatttgttg tatttttgta
     5641 tttattgtat tatttaataa tgtctattag ttctccattt tgtaaagttt taggtgtaat
     5701 tactatattg aactcttttt gcaaatcttt tataatatct aattcttttt ttgttattat
     5761 tgagattgtg ttaccacttc gatttcctct agcagttcga ccacatctat gcaggtattc
     5821 atttttactt tttggaaaat ccaagttaaa aacatgactt acatccacaa tatctagtcc
     5881 acgagcagat aaatcagttg taattaaaat ctttgcttta ccaagtttaa atttatttat
     5941 tgcattcttt ctatcttctt tttccatatt tccaaaaatt cctattgctt tgtaattatg
     6001 ataatttagt tttgatacta tgacttctat atttttttca ttatttacaa atacaatagc
     6061 tttttttgga tttgtagccg ctaatgcctt tctaagaaaa gtaaatttat cccttatctc
     6121 acctaataaa tatgagtgat tgatgttagg atttatttgt gatttttcct ttgtttttat
     6181 tatttcaaac tccttcataa gtttatcaca aatttttata gtgctatcag taagacttgc
     6241 agagcatcct ataatttgtc tatctcttaa cgttgttctt attatgtctt ctatacaagt
     6301 tatatttttt ccattcaata gattgtccac ttcatctaag acaatagttt ttatattatg
     6361 tgattttagc ttcttttgtt ttatcaaatc taaaactctt ccacaagagc caattactat
     6421 gtgtggtttt actgctttta tattttttat ttgtttttgt atattgacct ccccaattaa
     6481 agctaaagaa gtaacggaga gttctgcatt tttagccaat aattcaacct ggtttgtaat
     6541 ctgcattact aattcatgcg taggtgcaag tataagagct tgtgtttctc tttttgatgt
     6601 atcaattttt tcaaatatag gtaaaaggta tgctagtgtt tttcctgttc cagtctgtga
     6661 attaatcaat aaatccttat tttgaattat attccctata acaagtgact gaacttctgt
     6721 tggagaagtt atgtcttgtt ttttgagtcc atctattaga gtggaactta tttttaattg
     6781 ttcaaatgta ttcatatttt actccttata ttttaataat tatagtcaac tattttttat
     6841 tatacattgt ttattcaaat cgaactatat attcttttat aattatgtgt aatttgaaag
     6901 taaatatcaa ttatatttat acctttatat ttctgatacc tttgcatttt atagatttct
     6961 aaaataaaca aataaaagtt gtaat
//
LOCUS       DAGSRM010000137         6649 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.143, whole genome shotgun sequence.
ACCESSION   DAGSRM010000137 DAGSRM010000000
VERSION     DAGSRM010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6649
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.143"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(187..1083)
                     /locus_tag="MJE33_003420"
     CDS             complement(187..1083)
                     /locus_tag="MJE33_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBY9649329.1"
                     /translation="MKDKNTYFNRKGQENFIDIFLKDDIKKGANFKRHWHEHLQIYYF
                     IDGIAYLECGLDNFKVVPGNIVIANSNELHYLESLSDNLKFYSIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDSLILKCITTILDEYHNKEVGYELSIKANIYQLIVL
                     LLRSHIDKIYSEDEFIKRKYSLEKFECIFDLIEEKYMDKITLKELASRVNLSAHHFCR
                     TFKQITGKTTIDFINGIRIDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKRNYNI
                     SPTKYRNEHREVSNVILKEDMK"
     gene            1224..2651
                     /locus_tag="MJE33_003421"
     CDS             1224..2651
                     /locus_tag="MJE33_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBY9649330.1"
                     /translation="MTNSFKKDDNRKKITVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLASIEWVIASYSIIICSTLLFFGRLGDIIGKSRVFQVGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKESRGKALGILVTAVAIGNMVGPSVGG
                     FILSIFDWNVIFFINIPIGLIVIFLNTKFLPNSKKSSENMDKTGAILQFLGTTLFFSA
                     LISAQQTGLLNPYILIALLLSIIFIILFLILEKKHPQPLLDLEIFRNFKFSLNLICAL
                     TSFICIASSSILIPFYLQSTMKLPPIQAGLFMILSPLILAIFSPIFGNISDKIKSEKI
                     ILIGLLVMSFGFFLMSRLKESSALILFVIYISIISIGQAIFQPANNALIMSSCSRSKL
                     GVVGSINSLVRNLGQVIGITISTTLLYNFMSIKAGYRVNDYVINNDKIFVFGMRNVYI
                     IVTLVCLIGAILIGFYLFKYNKNEQ"
     gene            complement(2847..5441)
                     /gene="clpB"
                     /locus_tag="MJE33_003422"
     CDS             complement(2847..5441)
                     /gene="clpB"
                     /locus_tag="MJE33_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBY9649331.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNDFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSHRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSEDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETIIAKKLIAGDVYNGCTIVIDGKD
                     ENIEVLVK"
     gene            complement(5478..6074)
                     /locus_tag="MJE33_003423"
     CDS             complement(5478..6074)
                     /locus_tag="MJE33_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649332.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSKYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVNSNI
                     LKVFNNIAIYQYNILENLYTIDFKKSFIDKCQYLLIGEKRLIDEDLYLIAKSKEVFDF
                     FNSDGTFNLTLSFDIQKNENLLDSLLELRKSIIINERI"
     gene            complement(6091..6495)
                     /locus_tag="MJE33_003424"
     CDS             complement(6091..6495)
                     /locus_tag="MJE33_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HBY9649333.1"
                     /translation="MDKTLKKDLNQEYTESKSLVMNKSKYMNVCFKNYIKVVEGMYTK
                     RDLIPLKLINSMDKNNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYM
                     INPHIAYKGSRADKESLLAEYNQIKRPNKVSK"
BASE COUNT         2125 a         1078 c          702 g         2744 t
ORIGIN      
        1 cttatttttc cagttttagt ttcatattaa aatatacttt tcatagacac tgcattcttt
       61 catactcatt ttcacattta actatacaag tagtcttact catttatgta ctatacttta
      121 ttaatattta ttaagaaaaa ctcaaagatt taaaaatata tattttctat aaagtatatt
      181 tatggattat ttcatatctt cttttaaaat aacattacta acttctctat gttcatttct
      241 atacttagtt ggagatatat tataattcct tttaaataag cgactaaaat aatttatgct
      301 atcaaagcca catctcatag caatttctgt catatttaaa tctgtttcct ttaaataata
      361 tatagcttta tcaatcctga ttccatttat aaaatcgatt gtagtcttac ctgttatttg
      421 cttaaatgtt ctacaaaagt gatgtgcact caagttcaca cgactagcaa gttcttttaa
      481 tgtaatttta tccatatatt tttcttctat caaatcaaag atacattcaa atttttctaa
      541 agaatacttt ctttttataa attcatcttc agaatatatt ttatcaatat gacttcttaa
      601 taagagtact attaattgat agatattagc ttttatagat aactcatatc caacttcttt
      661 attgtgatat tcatcaagta tagttgtaat acattttaaa atcagagaat cattttgaat
      721 taaattatta aatacaatat gatttaaagc caatggtgct aagtacttgg tttgtaacaa
      781 atcaacttga ttactaaaaa ggaatgtggg ttcaatacga atactataga attttaaatt
      841 atctgataag ctttctaaat aatgtaattc attactgttt gctattacaa tatttccagg
      901 cactacctta aaattatcca atccacattc aagatatgct ataccatcaa taaaataata
      961 tatttgtaga tgctcatgcc aatgtctttt aaagtttgct ccttttttaa tgtcatcctt
     1021 taaaaatata tctataaagt tttcttgacc tttcctgtta aaatatgtat ttttatcctt
     1081 cattattccc ccctcagcaa aatagtgcta attataagca atatagttat tgtattcatt
     1141 atttcatatc gctattatat aaaatgtaac tatcattgta aacattaaca taatgcagtc
     1201 attataataa ggaggtaata aaaatgacaa actcttttaa aaaagatgat aatagaaaaa
     1261 aaatcacagt tttatttgta gtcattgcca tgacttttat ggcaacatta gattctagta
     1321 ttataaatgt tgctctccca gttcttgcaa gcaaactaaa tgtatctcta gcatctattg
     1381 aatgggtaat agcaagttac tcaattatta tttgttcaac actattattt tttggaagac
     1441 ttggagatat tattggtaaa tcaagagtat ttcaagtagg tactattctc tttacatcag
     1501 cttcattact atgtggatta agtaattctt taactttact tatagtttgc cgctttattc
     1561 aaggaatagg cgcttctgcc tacatggcaa acaatcatgg aattatcaca gaactatttc
     1621 caaaagaaag cagaggaaaa gccctaggga ttctagttac tgcagttgct attggaaata
     1681 tggttggacc atctgtggga ggatttattt tgtcaatatt tgattggaat gttatatttt
     1741 ttataaatat tccaatcggt cttattgtaa tctttttaaa tacaaaattt cttcctaata
     1801 gtaaaaaatc ttcagaaaat atggataaaa ctggtgctat acttcaattt ttaggaacaa
     1861 cattattttt tagtgctcta attagtgctc aacaaacagg attactaaat ccatatatat
     1921 taattgcact attattatca attattttta ttatattatt tttgatatta gaaaaaaaac
     1981 atcctcaacc gcttcttgac cttgaaattt ttagaaattt taaattttca ttgaatttaa
     2041 tttgtgctct tacatcattc atttgtattg catcatcaag tattttaatt ccattttatt
     2101 tacaaagtac tatgaaactt ccccctattc aagcaggttt atttatgata ctatcacctt
     2161 tgattttagc cattttttca cctatatttg ggaatatatc agataaaatt aagtcagaaa
     2221 aaattatttt aattggtctt ctcgttatga gttttggatt ctttctaatg tcaagattga
     2281 aagaaagttc tgcccttatt ttatttgtaa tttatatatc gataatatca ataggacaag
     2341 ctatatttca acctgctaac aatgctttga taatgtcaag ctgttctaga agtaagctag
     2401 gagttgtagg aagcataaat tcccttgtaa gaaatttagg acaagttata ggtattacta
     2461 tttctacaac acttttatat aattttatga gtattaaagc tggatatagg gtcaatgatt
     2521 atgtgattaa taatgataaa atatttgtat ttggaatgag aaatgtttat attattgtta
     2581 cacttgtttg cttaataggt gcaatattaa ttggctttta tttatttaaa tataataaaa
     2641 atgaacaata gtttttataa ttatattcaa attatgatta tattataaga aatttactaa
     2701 aatacttcct ttgagttaaa tttaatttgg acatattgac ttaaaaatgt tttattaatt
     2761 tgataaaata tttcttttcc tatagtttat aaataaaaat cagctgagca aaatatttag
     2821 tatattatgc tcaactgatt cttttattat ttaactaaaa cttcaatatt ttcatctttt
     2881 ccatctataa ctatagtaca tccattatac acatctccag caataagttt ctttgcaatt
     2941 atagtttcta atgtatttcc tatatatctc ttaagaggtc ttgctccata tacagggtca
     3001 tatccttctc tcaccataac atcttttgct gaatttgtaa cttcaatagt aatatctttt
     3061 tcttttaatc tattttttaa atctttcata aatatatcta tgattttctt gatattttct
     3121 tggtctaaag gcttaaacat tataatatca tctactctgt ttaaaaactc aggtttaaat
     3181 ctatgcttca tttcattcat tactaaattg ttagtagtct ctgttatatt tcctcctgct
     3241 tctaatagat aattacttcc tatattagaa gtcattatta taagagtatt tttaaagtct
     3301 actgtcttac ctttattatc agtaagtcta ccatcatcta atatctgtaa gaacatgtta
     3361 aatacatcct catgtgcttt ttctatctca tcaaatagta ttacagaata aggagcacgt
     3421 ctaactgctt ctgtaagttg accaccctct tcatatccca cgtaccctgg aggaggtcca
     3481 actagtcttg atacagcatg tttttccatg tattcagaca tatctattct tattatatta
     3541 tcttcactat cgaataaatt tctagcaaga gtttttgcaa gttcagtttt accaacacct
     3601 gtagggccta agaaaataaa tgaaccaatt ggttttcttt catcttttaa tcctgctctt
     3661 gcacgtatta ctgcatttga aactgctgta actgcttcat cttgtccaat aactctttta
     3721 tgaagttcat cttctagttt aagtaatttt tctctttctc cttcaactag tttagttact
     3781 ggtatacctg tccattttga tacaatgcta gatatttctt cttcagtaac ttcttctttt
     3841 aaaagagaat tttcagaacc atcattcata ttttcttcgt actgctttat ttgttcttct
     3901 aacttaggta tttcaccata tttaacctct gctgctttgt tgaaatcata ttctctttca
     3961 tacttttcag ctttaccttt agcttcgtct aattgagcct ttaagttttt aatatctaat
     4021 atttgactct tttctttttc atattttgct gtcatttcat catttttaga ttttaactct
     4081 gctatttctt tttgaatatc atccaatctt gatttacttt tatcatcatt ttctttaagt
     4141 aatgcttctc tttcagtttc taatgtaaat aactttcttc taacaacatc taattctgtt
     4201 ggcaatgaat ctatttcact acgaatcata gctccagctt catcaattaa atcaattgct
     4261 ttatctggta aaaatctatc ttgtatatat ctatgagaaa gttttgctgc tgctacaata
     4321 gcattatcat gtattcttac tccatgatgt atctcaaatc tttcttttaa acctctaagt
     4381 atagatattg tatcttcaac agtaggttct tctgcaatta caggctgaaa acgtctttca
     4441 agagccttat ctttttctat atactttcta tattcatcaa aagtagttgc accaatacag
     4501 ttcaattcac ctcttgcaag cataggttta ataagattac ctgcatccat tgaaccttct
     4561 gttttaccag ctccaacaat agtatgaatc tcatctataa acagtataat ttttccatca
     4621 gaactttgaa cttcttttaa aactgccttt aatctttctt caaattcacc tctatatttt
     4681 gcaccagcaa ttaaagctcc catatcaagt gagaatacaa tcttatcttt taaaccttct
     4741 gggacgtctc ctcttactat tctctctgct aacccttcta caattgcagt tttacctaca
     4801 cctggctcac caatcaatac aggattattt tttgttcttc ttgataaaat tcttataatc
     4861 cttcttattt cttcatctct cccaattaca gggtctaatt tatttttctt ggctaaatct
     4921 actaagtttg ttccatatct agcaagtgca tcatatgtgc cttctgggtc ttgtgtttct
     4981 actctttgac tacctctcac ttgtgataaa atatttaaaa agtcattctt atttatatta
     5041 tattgtttta atattttacc aacagctgat tttgattctg tttccatcat tgcaagcata
     5101 acatgctcta cacttatata tgaatcttta aattctttag aaatactctc tgcctttata
     5161 agcacctcat ttatttttct agtagctgat actccttgtg agtcagcacc ttctccatat
     5221 acttttggaa gcttatcaat ttcaaagttt acagtatttc tcaaagaatc aattgatata
     5281 ttcatttttt cgattatatt tggtatcaat ccatcctctt gattaataag tgctgagaaa
     5341 agatgaatta catctacttg ttggttatga ttttttactg cttcgttata agcctcattt
     5401 aaactttttt gaactcttaa agtcattttt tctacgtcca tataaatccc tccattttaa
     5461 tattatatcc aaataaatta aattctctca tttataatta ttgattttct aagctctaaa
     5521 agactatcaa gtaaattctc gtttttttgt atatcgaaac tcaatgttaa attaaatgtt
     5581 ccatctgaat taaaaaaatc gaatacttct tttgattttg ctattaaata caagtcttca
     5641 tctataagtc ttttttcacc tattaataaa tactgacatt tatctataaa actcttttta
     5701 aaatcaatag tgtatagatt ttctaaaata ttatattgat aaatagcaat attattaaac
     5761 acttttaata tgtttgaatt tacaaaattg taaatttgat tattgttata cagtgatatt
     5821 tgttcgtata tatattccct aaataaagga tgaatgtttt gcttaagttt taaagtaact
     5881 tcagctggta tttcaaaata aagcttccag tacttagagt tatttttttc taaaataaaa
     5941 gctactttgt tcaaactggt agaactaatc tcctttagtg tacaattaaa attatagtgt
     6001 ctcaaactag ttttttcatt ctcaaataca tagtcaaacc actgtagaac atttctatca
     6061 agttttaaac tcatataaaa accttcttcc ttatttacta accttattag gtctttttat
     6121 ctgattatat tctgctaata aactttcttt atcagctcta ctacctttat atgctatatg
     6181 tggatttatc atatactcac catttttcac tctttgtaaa aaatccattt ttttcagctc
     6241 cgtaaacaat gaagataaag aagtttttgg atattcaaat tcacaagata attcatataa
     6301 tgtttttcta atcttattat ttttatccat agagtttatt aattttaatg gaattaaatc
     6361 cctcttagta tacattcctt ctactacttt aatataattc ttaaaacaaa cattcatata
     6421 cttactttta ttcataacta aacttttgct ctctgtatat tcctgattta aatccttttt
     6481 taatgtctta tccaaattaa atatctcttc tcactctcct tatctctaat tgataatata
     6541 aatatattac aaaaaaatat gtatgtcaaa actaaaaatg caaaaagttt accttataag
     6601 taaactttta tttagctata agctaaactt tattattttt ttaatttag
//
LOCUS       DAGSRM010000138         6570 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.144, whole genome shotgun sequence.
ACCESSION   DAGSRM010000138 DAGSRM010000000
VERSION     DAGSRM010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6570
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.144"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            392..1666
                     /locus_tag="MJE33_003425"
     CDS             392..1666
                     /locus_tag="MJE33_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBY9649334.1"
                     /translation="MQFMDVSKYIEGANLKVKTKSGETVKRIYFNNSATPLVLKNVVD
                     NLNCEIPLLTYINAPGIISEKNTLKYENVRSTILKLIDGDEEKDSVIYVKNATEGINF
                     LAKFFKDENPDKFVITTAMEHMANYLPFKVNFPTKVVGITEKGELDLCQLENILKNYA
                     GNVSLVTVTGASNVTGITTPIYEIARIAHKYGAKILVDIVQVIQHKPFSMMPHECDEH
                     IDFVVFSAHKCYSPLNGGALVGPKKFFEKFNPVLYGSGSTKFVNDNKILYANVPQRFE
                     AGYPEYFGTLAMGKALNTLNKIGLNRISKYERELFLYAKEEMSKIPNIIIYGDKSNNV
                     IIPNISFNISNMYYKDVAKYLVDNFGIETGAGVVGADIYVQKLLGISPKEAYVRFMRE
                     NPVGLVRISLGMYNTFDEVDSFIHAIKTLAFK"
     gene            complement(1873..2190)
                     /gene="trxA"
                     /locus_tag="MJE33_003426"
     CDS             complement(1873..2190)
                     /gene="trxA"
                     /locus_tag="MJE33_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBY9649335.1"
                     /translation="MVKIVNNNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYEALG
                     NEMVEKANFLKVDIDQSIELAQKFEVSTVPTMLIFKDGKPVDRLIGFMPKENLKNKIE
                     SYM"
     gene            2457..3209
                     /locus_tag="MJE33_003427"
     CDS             2457..3209
                     /locus_tag="MJE33_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HBY9649336.1"
                     /translation="MDNIINELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQS
                     KVYENITKLLNNNIIINVGTDPIKYIPINPNELLKNKKKEFDNSMDNLKNSLQILNKS
                     KSVEYVLNIKGDKNILKKAIEMIKNAKQEIIISCPYEEILELKKELLIAYKKNINIEI
                     LLLENKSLKGLDNIHIHGGNKTTLENKMLYKGIILIVDNDEILTGNLSEGSEAISIWT
                     KNSNILYIGVQYIKHEIYISEQIKGGYKNEQN"
     gene            3196..4077
                     /locus_tag="MJE33_003428"
     CDS             3196..4077
                     /locus_tag="MJE33_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBY9649337.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTDTSNISDEIKQNVAKEMN
                     LSETAFVSESNIADFKVQFFTPGLEVDLCGHATIGTFHALLEEGKLNTNKSIFYQETK
                     AGVLSVELKKINNENIFMMEQNTPEFENLDNCRKELAQMIGVHEDRLLEYPIIKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVHHNILDKTSFIGEQGNFINRPGKVYVNITRENNNITS
                     VKIGGSAYTVLSGEIMY"
     gene            complement(4303..5658)
                     /locus_tag="MJE33_003429"
     CDS             complement(4303..5658)
                     /locus_tag="MJE33_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBY9649338.1"
                     /translation="MSSTVKKKGKFPMGFYICSTTFSFERAAYYASKYLIYIFLTTAI
                     VHGGLGIDKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDSQEELDTAFSVQYSFVNIGSFI
                     GTIAVGILYLKTFAKNGVLGFSQCFFIAAVLCVIGAIWFIYGWRFLGNAGKRPFKEGV
                     VAEKIEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVNMNVG
                     GFDVPLAWFDSLNSLVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFSRGVGAPETAKASILWIIMFGILLSLGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGKGYGYIYAFTLKFDMIKVYTIIPVILFVAAILLFLSDKTLSRLVEDD
                     K"
BASE COUNT         2525 a          945 c          824 g         2276 t
ORIGIN      
        1 ttttctaatt atatacaata tattttccat tatgttctaa tcaatttctt tactattttt
       61 acaactttat tagtcatatt cataattatt tctaattagt gtttaacatt ttatgtaaaa
      121 tttataaaaa aatgtatata aattaaaata agtgactata ctaaaaatat gatacggata
      181 aacaataagt tcaacctatc tcccccaaaa tcaagtgagc tacagtttcc cccaactgta
      241 gctcattttc gtgtaattta ataaacatct caacaagaat taattaacaa aaccccttat
      301 aaaaacataa catatattaa acaagctctt gattaaaact tgagcttttt taataaaaaa
      361 cagaatagtt tattagataa ggggtttttt tatgcaattc atggatgtat ctaaatacat
      421 tgaaggtgca aaccttaaag ttaaaactaa aagtggtgaa actgttaaaa gaatatattt
      481 taacaactct gccactccat tagttttaaa aaacgtagtg gataacttaa actgtgagat
      541 tcctttgctt acttatatta atgctccagg aataatctca gaaaaaaata ctttaaaata
      601 tgaaaatgta cgctctacta tcctcaaact tattgatgga gacgaagaaa aggattctgt
      661 tatttatgtc aaaaatgcta cagaaggaat aaacttctta gcaaaatttt ttaaagatga
      721 aaatccagat aaatttgtta ttaccacagc aatggagcat atggctaatt acctgccttt
      781 taaagttaat tttcctacaa aagttgttgg aattacagaa aaaggagaac ttgatttatg
      841 tcaattagag aatatactta aaaattatgc tggtaatgta tctctcgtta cagttacagg
      901 agctagtaat gtcacaggta taaccactcc tatatatgaa atagcaagaa tagctcataa
      961 gtatggtgca aagatattgg tggatattgt gcaagtaatt cagcataaac ctttttcaat
     1021 gatgccacat gaatgtgatg agcatataga ttttgttgtt tttagtgctc ataaatgtta
     1081 ttctccacta aatggaggag ctctggtagg acctaaaaaa ttctttgaaa aatttaatcc
     1141 tgtactatat ggctcaggtt ctaccaaatt tgtaaatgat aacaaaatac tatatgcaaa
     1201 cgtaccccaa cgttttgaag caggataccc agagtatttt ggaactcttg caatgggaaa
     1261 agcactgaac actttaaata aaattggtct aaacagaatt tcaaaatacg aaagagaatt
     1321 atttttatat gctaaagaag aaatgagtaa aatacctaat ataataatat atggagataa
     1381 atcaaataat gtaattatcc caaatatatc ttttaatata agtaatatgt attacaaaga
     1441 tgtagcaaaa tatttagttg ataactttgg aatagaaact ggagctggag tagtaggtgc
     1501 agatatttat gtacaaaaat tattaggtat aagtccaaaa gaagcatatg tcagatttat
     1561 gagggaaaat cctgtaggtc ttgttaggat aagcttagga atgtacaata cttttgatga
     1621 ggtggatagc tttatacatg ctattaaaac tttggctttt aaataagttt tttctattct
     1681 tttttatcaa taaatagaga gtagttttgt tttcacaaaa aagcaaatta tatcaacagt
     1741 caatttcatt agcaattaga cactcatttg taactataca aactatttta cactatacaa
     1801 acaaaaaact cattacaatg agtttttttg tttatatatg agatttattt taattacttc
     1861 aatttacttt aattacatat atgattcaat tttgtttttt aaattctcct taggcataaa
     1921 acctattaat ctatctacag gttttccatc cttaaatatt agcatagttg gtactgtaga
     1981 tacttcaaat ttctgagcta attctatact ttggtctatg tccaccttca agaagtttgc
     2041 tttttcaacc atctcatttc ctaaagcttc atatattggt gatagcatct tacaaggtcc
     2101 acaccaagtc gcaaagaaat ctacaactac taaaccatct ttattttcta cttcatttat
     2161 aaattcatta ttatttacta tttttaccat aatatacatc tcctttatta tcttcttgat
     2221 attaatatac tacgacctat ataacagttc aattaggcac ttttttgtaa ccgcctattt
     2281 ttaatagatt taatattatg ataaatagat ttataagatt aaatatttat aagactaaat
     2341 atttctaata tattttattt ttttcttaat atagttgaca ttttttccct taaattataa
     2401 aatcaaatta ataactacat aggtagttac taatctttta tcaaaggagt ttctatatgg
     2461 ataatattat taatgaatta caaaatatag gatttactaa atacgaatct caaatataca
     2521 tatcattatt aaaggaaagc cctttaacag gatatgaaat aagtaaatta tcaggtgtac
     2581 cacaatcaaa agtctatgaa aatataacaa agctattaaa caataatata attatcaatg
     2641 ttggaactga ccctattaag tacataccaa taaaccctaa tgaattatta aaaaataaga
     2701 aaaaagaatt tgacaattct atggataatc taaagaatag cttacagata ttaaataaaa
     2761 gtaaaagtgt agaatacgta ttaaacataa aaggagataa aaatatatta aaaaaagcaa
     2821 tagaaatgat aaaaaatgca aaacaagaaa ttataatttc ttgcccttat gaagaaattc
     2881 ttgaattaaa aaaagaactc ctaattgctt acaaaaaaaa tattaatatt gaaatactac
     2941 ttttagaaaa caaaagtctg aaaggtcttg ataatattca tattcatgga ggtaataaaa
     3001 ctacacttga aaataaaatg ctttacaaag gaattatttt gattgtagat aatgatgaaa
     3061 tattgacagg aaacttatca gaaggcagtg aggccatttc aatttggaca aaaaactcta
     3121 acattttata tattggagtt caatacataa aacatgaaat ttatatttca gaacaaataa
     3181 aaggaggtta taaaaatgaa caaaattaag attaaacaag ttgatgcttt tacaactata
     3241 ccttttggtg gaaatcctgc tggtgtcgtt actgatacct ctaacatttc tgatgaaatt
     3301 aagcagaatg tggctaaaga aatgaattta tcagaaactg catttgtttc agagtcaaat
     3361 attgcagatt ttaaagtaca atttttcaca ccaggtttag aagttgattt atgtggtcat
     3421 gcaacaatag gtactttcca tgcacttttg gaagaaggga agcttaatac taataaaagt
     3481 atattttatc aagaaacaaa agctggtgta ttatctgtag agcttaagaa aataaataat
     3541 gaaaatatat ttatgatgga acagaataca cctgaatttg aaaatttaga taattgcaga
     3601 aaagaacttg cacaaatgat aggtgtgcat gaagatagac ttcttgaata tcctattata
     3661 aaggtaaata ctggactttg gtggcttgta tttggtctaa aatctttaga tgatttaaaa
     3721 aatatctctc cagatttaaa taaaatcaaa aaatttagtg gtgataataa tataattggt
     3781 ataactccat tttgtataga aactatagat aaaaagtgtg actaccattt aagatctata
     3841 gcaccatatg ttggagttac agaagaccct gtatgtggaa ctgggaacgc ttgcgtatca
     3901 tcttatatag tacatcacaa tattttagat aaaactagtt ttattggtga gcaaggtaac
     3961 tttataaaca gacctggaaa ggtatatgta aatataacta gagaaaacaa taacataact
     4021 tctgtaaaaa ttggaggaag tgcttatact gtcttaagtg gtgaaattat gtattaatcc
     4081 catacctaat aaaaacaaat aaaaatacta gctaactaaa gctagtattt ttatttgtta
     4141 tttttattta ataatcatac tctaacaaat attataatta tatatatcat aatattgtat
     4201 ttagaagtaa aattttattt ttattattaa ttagttataa atcaatctaa tacaacataa
     4261 ttacagtaga ttaacctata aaacataata attgcttact gattatttat cgtcctcaac
     4321 aagtctactt aatgtcttat cagataagaa taataatatc gctgcaacaa atagtattac
     4381 tggaattata gtatatactt taatcatatc aaatttaaga gtaaatgcat atatatatcc
     4441 atatccttta ccagctataa aagtagcaaa tgtccatact cccataagaa cacctagaag
     4501 cttcttaggt gcatacttac ttacaaaaga gttaccaagt ggtgagaata acatttcacc
     4561 aagacttaaa agaataccaa acatgattat ccatagtata ctagctttag cagtttcagg
     4621 agctccaact cctcttgaaa attctgcacc aacaagcatt aagaatgcaa gacctaagaa
     4681 tattaatcca agacctgtct ttttaaataa actcatatct ccttgtggtc tactagcaag
     4741 tttaaaccat aatgctccaa gaactggtcc taatactata catacaagtg aatttaatga
     4801 gtcaaaccaa gcaagaggaa catcaaatcc tccaacattc atgtttacaa atgctgcacc
     4861 ataatcataa actgcaagat aagttagata ccagaatacc cagaaaatta cagagaagaa
     4921 tgatataagt ataatagccc aaattctctt cttatccatg cttgtaagag gagacttatc
     4981 tttttcttca attttttcag caactacacc ttctttaaaa ggtctctttc ctgcattccc
     5041 aaggaatctc catccatata tgaaccatat tgcacctatt acacaaagta cagctgctat
     5101 aaagaaacat tgagaaaatc caagtacacc attttttgca aatgttttaa ggtaaagaat
     5161 accaactgct atagtaccaa taaaagaacc tatattaaca aaagaatatt gtactgaaaa
     5221 tgctgtatca agttcttctt gactatcaaa aagctgtcca tttaccgcag atacgttacc
     5281 tttaaagaat gcagtaccaa ttgaaactaa gattatcatt gcatttacca tagaaactgt
     5341 agttgcaata gaaccaagat agtatcctac ccccatgata agcataccta taggtatagt
     5401 atatctagct cctatccatc tatctgatat atacccccca attataggtg caaggtatgt
     5461 gaatgcaaca aggttggctt gcattatagc agcttgtcct ttatctattc caagaccacc
     5521 atgaactata gctgttgtaa ggaatatata tattaagtac tttgaagcat aatatgctgc
     5581 tctttcaaat gagaaagttg ttgagcagat ataaaatccc ataggaaatt tacccttctt
     5641 ttttactgtt gagctcataa ttttaaatcc tttcttaatt gtatttttat tttttacgtt
     5701 ttgtccaaat tctttctttt atgaaagata taatattatt attttgtaaa acaatttgta
     5761 attattatta agataattgc gcatttattt gtagagtaca tattctcatg gaataaaatt
     5821 aatacacttc ttaatcataa atttaaatta tataaattca ctattttcag atatagttat
     5881 aaattaatat attctcatct aataccatga aaaaaccttc gccatatgca attcttattt
     5941 tttacgtgat agtattttac aaactaaaat cccccttttg tagcaactta tgtaattaca
     6001 ttttagtgct tttttaaagg agtctcgaat aaagcaacat atattttgtt caaataatgt
     6061 ttttatttat tttttaaatt tatatattac ttcacccaac caattctcaa cttaaataac
     6121 taaactacta tctattcaca gattaggctt atttcttaca cattagttta taataaacac
     6181 tgtgaatcta aacataaaaa ctgttatttt aaagtaatta cccatatatt cgctttgata
     6241 ctatttattg aggctatact ctttatgtat aatatattat ttataaaata cctcaaaata
     6301 tattattcat aaaatacctc aaactgaaaa ctttttcacg aatatttatt tttcacctcc
     6361 ccttttttat ttcaccatgt aaatcgtacc ataaatatgc acttatatgc aagaaaaaaa
     6421 tctattaatc gacagtaaat aattagaaaa attcaaatta tatttatcaa ttatattata
     6481 taaaatattt cctatgaaaa tataaattaa attaatgttc agaatagttt ttagatatga
     6541 aatcttgatt tttttattac tttacttatt
//
LOCUS       DAGSRM010000139         6378 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.145, whole genome shotgun sequence.
ACCESSION   DAGSRM010000139 DAGSRM010000000
VERSION     DAGSRM010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6378
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.145"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            313..1257
                     /locus_tag="MJE33_003430"
     CDS             313..1257
                     /locus_tag="MJE33_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HBY9649339.1"
                     /translation="MKFLRIEDEVIYSKKRYKLKREIDKLFLDEEYFQIEPQLFEEYD
                     EFTTINNKIPEESMVKVVNGKVMVLRADITTSIIKSLVPRWEDGLKLKLFYNSSIYKN
                     KNTVGIKEIRQIGCEYLGEASVEADREVVKLALKILEKYNNNFILEVGSSKYIHGLLE
                     ELNLNKNCENQIKNLLYTKNTHELKVYIEELKIKREVKELLSNILSLQGNLYNVIEKS
                     NKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGYYPNSY
                     KEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
     gene            1279..1905
                     /locus_tag="MJE33_003431"
     CDS             1279..1905
                     /locus_tag="MJE33_003431"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HBY9649340.1"
                     /translation="MDYLTIALAKGRIEGESFKKFKKMGLGDSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            1939..2988
                     /gene="hisC"
                     /locus_tag="MJE33_003432"
     CDS             1939..2988
                     /gene="hisC"
                     /locus_tag="MJE33_003432"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBY9649341.1"
                     /translation="MREKESIRELKGYEPNHVNCKVKLDANEGSKRLFKYLIKEISDS
                     DIDLNLYPEDSYSDLKESIIDYINISGVNKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVINIDNVIEKVKENNPKIVIVCNPNNPTG
                     TILKREEIIKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEEREVLYK
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEN
                     SMFVGAIRDILKKEK"
     gene            2991..3569
                     /gene="hisB"
                     /locus_tag="MJE33_003433"
     CDS             2991..3569
                     /gene="hisB"
                     /locus_tag="MJE33_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HBY9649342.1"
                     /translation="MRIWKVERNTLETQILVELNIDGSGKAEIDTGIGFLDHMLTLMS
                     FHGKFDLKVICKGDTYVDDHHSVEDIGIAIGEAFKNALGDKKGIRRYSNIYIPMDESL
                     SMVAIDISNRPYLVFNAKFDTQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            3571..4176
                     /gene="hisH"
                     /locus_tag="MJE33_003434"
     CDS             3571..4176
                     /gene="hisH"
                     /locus_tag="MJE33_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HBY9649343.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSDIDEIKQADSLILP
                     GVGAFRDSISALDKLGLIPIIKEHVSKGKFMIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDISLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELIAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            4173..4895
                     /gene="hisA"
                     /locus_tag="MJE33_003435"
     CDS             4173..4895
                     /gene="hisA"
                     /locus_tag="MJE33_003435"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HBY9649344.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKVNVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGVNTKIIEDIANNIDIPIQVGGGVRDKEKVKSLINAGVTRVIL
                     GSIAIENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEVVSNVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFDIYERIAKETSLNVIASGGVTSIEDVKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLGE"
     gene            4877..5635
                     /gene="hisF"
                     /locus_tag="MJE33_003436"
     CDS             4877..5635
                     /gene="hisF"
                     /locus_tag="MJE33_003436"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HBY9649345.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGA
                     DELVFYDITASNEERKTSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKDMGVEVRL"
     gene            5663..6331
                     /locus_tag="MJE33_003437"
     CDS             5663..6331
                     /locus_tag="MJE33_003437"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HBY9649346.1"
                     /translation="MDNKCNNVYSDEVEKFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKDAIKKTLKDKVACYFSRSRQELWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDSSKMELEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEIDEIKKELL
                     KRRK"
BASE COUNT         2610 a          540 c         1258 g         1970 t
ORIGIN      
        1 attaatagta acaaaaaact gtaaatttag aatattaata aaaattattt gggaggaaat
       61 tatcatggtg agattaaata tagtaaaatt aaatacaaaa ttaaatcatc accatcatca
      121 tagattaaag ggattgtaat atgaaattaa ttcatagaca caagccctgt ttttagtgta
      181 attggtcttg tatctatgat gggaagtaat tttaagagaa aattatagcc atataggtat
      241 aaagcctatg tggctttttt attgttttaa atttgcaaat ataagagtaa atttaatgaa
      301 gagggtgaga atgtgaaatt tttgaggatt gaagatgagg ttatttattc taaaaaaaga
      361 tataaattaa aaagggaaat agataaactt tttttggatg aagaatattt tcaaatagaa
      421 ccacaattat ttgaagaata tgatgaattt acaactataa ataataaaat tccagaagaa
      481 tctatggtaa aggttgtaaa tggaaaagta atggtactta gagctgatat aactacaagt
      541 ataataaaaa gccttgtacc aagatgggaa gatggtttaa agcttaagct tttttataat
      601 tcatcaatat ataaaaataa aaatacagtt ggaataaaag agataagaca aataggttgt
      661 gagtatctag gagaagcctc tgtagaagca gatagagaag tagtgaaatt agctcttaag
      721 atacttgaaa aatacaataa taattttatt ctagaggttg ggagtagcaa gtatattcat
      781 ggtttgttag aagaattaaa tttaaataag aattgtgaaa atcaaattaa aaatttacta
      841 tatacgaaga acactcatga actaaaagta tatattgaag aattaaaaat taagcgtgaa
      901 gtaaaagagt tattatcaaa tattttaagt ttacaaggga atttatataa tgttattgaa
      961 aaatcaaaca agttctattg taataataag atgaagcaag ctcttgaaga attaaagcaa
     1021 gtaaataatt tgattgagga atgtaatttt ttagataagg ctagatttga tttatcaatg
     1081 attactatgc ttgactatta tgaaggaatt atgtttagag gatactaccc aaactcatat
     1141 aaagaaattc ttagtggtgg aagatatgat tctttaacta aagagtatgg aaaagaaatt
     1201 ccagccattg gatttacact aagtgttgat gagttaatga agtatgtata taagtaaagt
     1261 ttagaaggag ggattgcttt ggattatttg actatagcac tagcaaaagg acgaattgaa
     1321 ggggaatcat ttaaaaaatt taaaaaaatg ggtctaggag acagtataga cacagatacg
     1381 agaaaactaa tttttaaaga tgaagaaaat aaaattatat atatacatgt aaagccatca
     1441 gatgtagtta catatgttga aaaaggagtc gctgacctcg gtatagcagg taaagatact
     1501 attttagaga atgagacaga tgtatatgaa atatatgatt taggttttgg aaaatgtaaa
     1561 tttgctgtag caggattaaa gggagatagc atatatagag aagatgaata tttaaaagtg
     1621 gcaacaaagt atcccaatat agcaaaaaag tattttaaag agaaaggtca aaaaattgaa
     1681 ataataaaat taaatggttc tgtagaacta gctccaatag tagggttgtc agatgtaatt
     1741 gtagatatag ttgagactgg caatacactt aaagcaaatg gtcttgaaat attagaagac
     1801 atatgtaata taagtgctag aattatatca aatagagcta gctatagatt taaatatgaa
     1861 caaattcaga atataattcg tttatttgaa gaacttgata attaaaatat aattgagctt
     1921 tataaggagg gatttaagtt gagagaaaaa gaaagcataa gagagttaaa gggatatgaa
     1981 ccaaatcacg taaactgtaa agtaaaactt gatgcaaatg aaggaagtaa gaggttgttt
     2041 aaatacctta ttaaagaaat ttcagatagt gatatagatt taaatttata tcctgaagat
     2101 tcttatagtg atttgaaaga atctataata gattacataa atatttctgg agtaaataag
     2161 aaaaatcttc tagtaggaaa cggttcaagt gaaataattg atttaataat acatactttt
     2221 gtagataaag atgaagtaat attgagtttc tctccaagtt ttagtatgta ttctatatat
     2281 agtcagataa atgggtctaa atttataggt gttgaaagtg atgaaaattt agttataaat
     2341 atagataatg tgattgaaaa agtaaaggag aataatccta aaatagtaat tgtatgtaat
     2401 cctaataatc caactggaac aatattaaag agggaagaga taataaagct tttagattca
     2461 actaatagtc tagtggtttt agatgaagca tatatggatt ttggagaaga aagcatgtta
     2521 agtgatgttt ttaagtatga taatctaata gttcttagaa ctttatcaaa ggcatttgga
     2581 ttggctggaa taagaacagg atatatgtta tctaatagta gtttaataaa ttctgttgag
     2641 aaagtaagac ctccatataa tttaaattca ttatcagact ttattgctac aagagcactt
     2701 agaaataaag atgtggtaaa ggcttatata aaggaagtaa aagaggaaag agaagtttta
     2761 tataaagaaa tgattggtat gggaattaaa gcatataaat cacaagcaaa ttttatatta
     2821 ttttattctg aaatcgaaaa tttatcacaa aaacttattg atagaggtgt tttaataaga
     2881 aaatttggtg gaaagttaga aaattattac agagttacaa ttggtgataa agaagaaaat
     2941 tctatgtttg ttggagctat aagagatatt ttgaaaaagg agaaataggt atgagaattt
     3001 ggaaggtaga aagaaatact cttgaaacac agattttagt agaattaaat attgatggtt
     3061 ctggtaaagc tgaaatagat actggaatag gatttttaga ccatatgctt accttaatgt
     3121 catttcatgg caagttcgat ttaaaagtga tttgtaaggg tgatacatat gtagatgacc
     3181 accatagtgt agaagatata ggtatagcta taggtgaagc atttaaaaat gctctaggtg
     3241 acaaaaaagg aatacgaaga tattctaata tctatatacc tatggatgaa tctctatcaa
     3301 tggtagcaat agatataagc aatagacctt atcttgtatt taatgctaaa tttgataccc
     3361 aaatgattgg tagtatgagt actcaatgct ttaaagaatt ttttagagct tttgtaaatg
     3421 aatcgagagt aacattacat atcaatctct tatatgggga aaatgaccat cataagatag
     3481 aaagtatttt taaagcattt gcaagagctt taaaagaggg tagtgaaata gtttctaatg
     3541 agatagcatc ttcaaagggg gttttgtaga atgaatataa tagtagacta tggtcttgga
     3601 aatatagatt ctgtgtctag gggttttaga aaagctggaa tagaaactaa aatttctagt
     3661 gatatagatg aaataaaaca ggcagattca cttatacttc caggtgttgg agcttttaga
     3721 gattctataa gtgccttaga taaactagga ttgataccaa taataaagga gcatgtttct
     3781 aagggtaaat ttatgatagg tatatgtcta ggaatgcaat tactttatga aaaaagttat
     3841 gagtatggag aatatgaagg attaggactt ataaaaggaa gtattgataa actggatata
     3901 agtttaaaag tgccacatat gggatggaat aatctaaaat ttaataaggc aaatgatgat
     3961 atacttaaat atataaatga agatgattat gtatacttcg ttcactcata ttatgcaaat
     4021 tcatcaaatg aagaactaat tgcattttca gaatatgaaa agaaaatccc tgctattgtt
     4081 agaaaaggca atgtatatgg tatacaattt catcctgaaa aaagtggaga agtagggtta
     4141 aatatattaa gagcatatgg ggagatgata aaatgataat atttccagca atagatataa
     4201 aagataataa gtgtgtaaga ctcacacaag gagaatttga caaagtaaat gtatattatg
     4261 ataatccatt agaagtagca tataaatgga aaaatgaagg agcagaatat atacatatag
     4321 tagatttaaa tggtgctaga agtgaatttg gtgtaaatac aaaaataatt gaggacatag
     4381 caaacaatat agatatacct attcaagttg gcggtggagt tagggataag gaaaaagtca
     4441 aaagtttaat aaatgctggt gttactagag taatattagg cagtatagcg attgaaaatt
     4501 tgaatttagt tgaagaattg gtcaatgaat ataaagagaa aattgtagtt tctatagatg
     4561 ctaaagatgg aaaagtagca gttaggggat gggaagttgt aagcaatgta gactcattaa
     4621 cattatgtaa acaacttgaa aagataggtg tacaaactat tgtttataca gatatatcaa
     4681 aagatgggat gttgcaaggt ccaaattttg acatatatga aagaatagca aaggaaacat
     4741 cactaaatgt tattgcttct ggaggcgtaa cttctataga agacgtaaaa agactaaaag
     4801 ctatgaactt atatggtgca ataataggta aagcgcttta tgataaaaag atagacttta
     4861 aggaggcaca acaattatgc ttactaggag aataatacct tgtctagatg tgagaaatgg
     4921 aagagttgta aaggggaaaa aatttaaaga tatagtagat gttgatagtc cagaagtact
     4981 tggaaaattt tatagtgatt gtggtgcaga tgaacttgta ttctatgata taactgcatc
     5041 aaatgaagag agaaagacat ctttagaatt tgtgacaaaa gttgcagaaa atataaatat
     5101 accattttgt gttggtggag gagtaaataa attagaagat ttcactgata ttttaagaaa
     5161 aggtgctgat aaagtttcta taaattcatc agcagtcaaa aatcctgaat taataaggga
     5221 agcttcatta aaatttggag cacaatgtgt tgttttatcg atagatgcaa agaaaaatga
     5281 agagggttca tggagcgtat acgtaaaagg tggaagagaa aaaactaatc tggatgcaat
     5341 tgaatgggct gttaaaggtg tagaacttgg agcaggagaa atcgttgtaa atagtatgga
     5401 tgaagatgga atgaaaaatg gatatgatat agaattatta tcaaagataa cttctttagt
     5461 aaatgtacct gttatagcat ctggaggcgc tggaaaaaag gaagattttt atgaggcggt
     5521 taataaatcc aatgtagatg gaattttagc agcatctgtt tttcattttg gcgagataaa
     5581 aataaacgat ttaaagaaat atttaaaaga catgggtgta gaagtaagat tataagttaa
     5641 atactaaaag gagattagaa atatggataa taagtgtaac aatgtataca gtgatgaagt
     5701 agagaaattt ataagaagta taaagtttga tgataaaggg ttagtccctg ttgttgttca
     5761 agaggtagtt agcaaagatg tactgatgtt agcatatatg aataaagatg ctataaagaa
     5821 aaccttaaaa gataaagtag cttgctattt tagtagaagt agacaagaac tttgggttaa
     5881 aggtgagaca tctggtaata ctcaaaaagt agttaagatg tcttatgatt gtgatgtaga
     5941 tactatacta ctttttgttg aacaaactgg tgtagcttgt catacaggca attattcttg
     6001 tttttacaga gacttgtttg atgatagttc aaaaatggag cttgaagtac aaacaaatat
     6061 attgaaagaa ttatatgatt taatcaatga aagaaaaaat aatccagttg aaggttctta
     6121 tactaattat ctttttgaaa aaggaataga taagattctt aaaaaagtag gtgaagagag
     6181 tagtgaggta attatagcat caaaaaatac cgataaaagt gaacttattt acgaaattag
     6241 cgatttagta tatcatacat tggttttaat gatagaaaaa ggtgttgaaa ttgatgaaat
     6301 aaaaaaagaa ttacttaaaa gaagaaaata gaataagata gattaatggg taataagaaa
     6361 atataccgct tttaataa
//
LOCUS       DAGSRM010000140         6285 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.146, whole genome shotgun sequence.
ACCESSION   DAGSRM010000140 DAGSRM010000000
VERSION     DAGSRM010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6285
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.146"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            213..863
                     /locus_tag="MJE33_003438"
     CDS             213..863
                     /locus_tag="MJE33_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HBY9649347.1"
                     /translation="MKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFII
                     EGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNVF
                     EEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCISNKQENNVCNIKAI
                     RNFSEIYSISRKSRYHSLLKLTEKEIVKKMGNTYEILNYDELHKLAFEVKEFLEDE"
     gene            complement(1003..2379)
                     /locus_tag="MJE33_003439"
     CDS             complement(1003..2379)
                     /locus_tag="MJE33_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBY9649348.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVSNIRKVTGLTCVLA
                     IVLSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAVSTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYLYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAIGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(2395..4308)
                     /locus_tag="MJE33_003440"
     CDS             complement(2395..4308)
                     /locus_tag="MJE33_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBY9649349.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIEMESYLR
                     ELEVKISEESTKTNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELQPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLEIEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLASLYDEWEEFM"
     gene            4444..5076
                     /locus_tag="MJE33_003441"
     CDS             4444..5076
                     /locus_tag="MJE33_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBY9649350.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            5215..5754
                     /locus_tag="MJE33_003442"
     CDS             5215..5754
                     /locus_tag="MJE33_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBY9649351.1"
                     /translation="MVFVEELWKKAIEFHGHECPGLAIGFKAALAAKKYLGCKQSGDE
                     EIVCISENDACGIDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FEMDREGYKRYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFDKYERFYEEY"
     gene            complement(5930..6100)
                     /locus_tag="MJE33_003443"
     CDS             complement(5930..6100)
                     /locus_tag="MJE33_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBY9649352.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDAGSCIDCGSC
                     AGACPVDAPQPE"
BASE COUNT         2338 a          857 c          869 g         2221 t
ORIGIN      
        1 ataaattcta ttgataatat aattttgtaa atatagaatt atattaaaat aaattatata
       61 tgaaaatata tatgtataag tttattctgc gtgagtagaa tgaatttgtc aagtttaatt
      121 tagttgtaat tttatctttt attaaaaata atataaatta aaacatataa ataaaatatt
      181 ttatacaata tattgtaaag gaggtaactt tgatgaaagc agaaaaaaca agtatatatc
      241 caattattaa aaataattta aacaaaatat caattgttga atataaaaag ggtcaaagat
      301 ttgtagcatc agataaagac ttacaagaag tgttctttat aatagaggga gttgcattag
      361 tggagtgtac tactagagga ggaaataaat ttcttgttga cattgtacct gaaaatgaat
      421 ttataggcaa aattagttac atatatgaac ataatttgaa atgtgatata tttgcaaaga
      481 ctaatataaa attatttcga tttgaaaaaa atgtatttga agagttttat ctcaaaccag
      541 attttattgc actatttcat agaaaatgta caagaagaat atatgaatta tataaaacca
      601 gaatggtaag agaactattt tcttgcacag aagttattgc ctattgtata tcaaataaac
      661 aagaaaataa tgtctgcaat ataaaagcta ttcgtaattt cagtgaaatc tattcaatta
      721 gtagaaaaag tagataccat tctctgctaa agttaactga aaaggagata gtaaaaaaaa
      781 tgggtaatac atatgaaatt ttaaactatg atgaattaca taaattggca tttgaagtaa
      841 aagaattttt ggaagatgag taaaaattgt tacttaatca aaataaagta taataatttc
      901 tgtaaaaaat agaaatagtc tcactgaaaa tcaaaaatac tttagttatt ttcaatgaga
      961 ctgtttagaa atattgtata cttataatgt atagtataat tattaatcaa tttcctttac
     1021 tatattaata gcccattttt tagataatgc tctaggaaca ccaaatataa acttaaccac
     1081 ttcttcacta taagtgagca aaacgagtac aaatataggt gcacctttaa atgcagctat
     1141 aaaagttaga ggaatagaag ctaaccacat acatcctaat tctaaaaata gagcatattt
     1201 agtatctcct ccacttctta aaataccaat tataaccaat gtattaaaag atttaagagc
     1261 cattagtatt cccattataa agaatatctt agttatatca gaagctaagc tatcagaaac
     1321 actaaacatt tttaataata atggtatatt taaaattaaa ataattccaa gtattaaacc
     1381 agctgaaaat acaagtatag aaaacttttt agcatattca atggctcttt taatatggtc
     1441 agctccaagt tcatttccaa gcataataga tgcaccagat gcaatacata cagctgtcat
     1501 tatgaagaaa ttacctgtac tggttgctat ctggctagct gctatagcag aagtaccagc
     1561 tcttgcatat gcaacagaat acaggacagt tccaattgcc catacagagt catttacaaa
     1621 tataggaaag ctttttgatg aaaaagattt tataaaaatt ttatcaatag cttttagatt
     1681 ttttagacca aactttaata tatagtcttt tttataaagg taaacataag taatcattaa
     1741 aattaattca catattctag caataacagt agctagagca gcacctttta cacccaatgc
     1801 aggaagaccg aagttaccaa atataaatcc ataatttaaa ataacatttg ttacaagtgc
     1861 aaatgcacta caaatcatac ctaacttagg atttctaaca cctcttgaac cagtactaaa
     1921 tacagtactt acagcaatta atggataact aaatactgca atactaaagt aatctataca
     1981 aagttttacc acctcagaat catacgaaaa tatgtgtata attggctttg gtgttagtaa
     2041 agcaggaata acaaatacaa aacttaaaac aattgccaat acacaagtaa gacctgtgac
     2101 ttttcttata ttactcacat ccttctttcc ataaaactgt gaaataaaaa ctccagcacc
     2161 tcctgtgata ccagataagg acatattaaa aagaaagaaa aattggtttg caactccaac
     2221 tgaagctaca gatgtttccc ccaaactact tatcataatt gtatctatta catttacaga
     2281 agtagaaatt aagttctgta tgataatagg tatacataaa gatagaagta ttttataaaa
     2341 tcttttattt tcgtctttta aagaaaatag ttcttgcaaa taatcctctc ctttttacat
     2401 aaattcttcc cactcatcat atagagaggc aaggttattt tctaaattaa ttttttcttg
     2461 gctaacttct ttagctttat ctggattaga gtatacttct tcttgacata aaagtatatc
     2521 taatttttca atttcaatct ctaacttttc aatttctttt tctatatttt gtctttttat
     2581 tctatttttc ttttctattt ctctttgttc tctctctttt cttttttcat cttttatttg
     2641 agttctagtt ttttcttctt tttcttctat aatactcatc tcttggattt gccttttttt
     2701 atctatataa taatcataat ttcccagata ttcagtaatt ccattttcat ctaaaactaa
     2761 aattttatca acaacagtat ttagaaagta tctatcatgt gatattgtaa atatagtccc
     2821 atcataatta gttaaggcct cttcaaggac ttccttagaa tcaatatcta ggtgattagt
     2881 aggctcatct aataacaata agtttgcatt tgatagtata agtttaagta tggcaactct
     2941 agctctttct ccaccactca atgtagaaat cttcttaaaa acttcttcat ctacaaataa
     3001 aaatgcccct aacatatttc tcaaagtagt ttgagtaaga tgagtattat tttcccaaat
     3061 ctcatcaatt attgtgttat ctaaattaag agttttttgt tcctgatgga agtaggaaac
     3121 atgtacatta gttccaaatt taatattgcc actaagaggt tgtagttcat tagttattat
     3181 tttaaataat gtagatttgc caacaccatt tgcacctata agtgcaactt tttcccctct
     3241 atatatatct aaatccaagt ctttaaaaag tattctttct ccataaccca tagatacatc
     3301 tctaatttga agaacatcat taccactctg gacagaaggc gtaaattgta tacgagcttt
     3361 tttcctataa gcttctggtt tatctaaaac atctactttg tctaatgctt tttctttact
     3421 tcttgcacgc tttaggtgtt tttctctacc ataagctttt agtctttcaa ttgattcttc
     3481 ttgctttttt atttcttttt gttggtcttc aaatttctta agctctagtt ctttttctat
     3541 tttagataac tcaacaaatt tagagtagtt accattatat actttaagct ttttattgtg
     3601 aacttcaaaa attctattta cggattggtc taagaaatat ctatcatgag atattaacat
     3661 gacagtccct ttatattgtt ttaaaaatac ttctaaccat tctattgctt cagagtctaa
     3721 gtggttagta ggttcatcaa gtaacaatag agttggtttc ttaagaagta attttcctaa
     3781 aagaactcta gttttttcac caccagaaag tatgcttatt ggcttatcca tatcattgtc
     3841 actaaatcct aatcccttta gaactccttt ggcttcagat ttatatccat atccatttaa
     3901 atctgaaaat aattctagtt tatgtgaata ttcatccatg attttttcta taattggaga
     3961 gtttgttttt gtactttctt cagatatttt aacctccaat tctcttaaat aggattccat
     4021 ttctatcaaa tttttaaaaa cttctagtac ttcttccaaa atagtattat ccgaataaaa
     4081 attggtattt tgttctaaat atccgatttc acaatcttta gaagtgtaaa tatctccact
     4141 atcatatcca taaattcctg aaattatttt aaataaagta gtttttccag taccattaac
     4201 accgattata ccaattttat cgccttcatt tactgtaaaa ctaatatttt ctaatataga
     4261 atctatacca aaacttttat ttaagttatt acatgacaaa acaatcataa atttctacct
     4321 ccttgaacta tctaaaatta taatgttttg atataatata atcaaggtta tatgtattag
     4381 ttttgaaaaa gaaatatttt attgtgaatc aaacaaaaaa gaggacactt ttaggaggtg
     4441 aagatgttgg gaaataaaaa tatatcaatg gcagttataa gaaggctccc aaaatatcat
     4501 agatatcttg gagacttatt agatagggat atacaaagaa tatcttctaa agaattgagt
     4561 gatataatag ggtttaccgc ttctcaaata agacaagatt taaacaactt tggtggattt
     4621 ggacaacaag gatatggtta taatgtagaa gctcttcata ctgagatagg taaaattctt
     4681 gggttggatc gaccatacaa cgcagttctt gtaggagcag gtaacttagg acaagctata
     4741 gccaattatg caggatttag aaaagctgga ttcgagataa aagctttatt tgatgcaaat
     4801 cctagaatga taggtttaaa gataagagag tttgaagtat tagattcaga tactttagaa
     4861 gactttataa aaaacaataa tatagatatt gctgtattat gtatacctaa aaatggagca
     4921 caagaagtta ttaatagagt tgtaaaagct ggaatcaaag gtgtatggaa ttttgcacct
     4981 ttagatttag aagttccgaa aggtgttata gttgaaaatg taaacttaac agaaagttta
     5041 tttaccttat cgtatttaat gaaagaagga aagtagacaa cttaaacata ttaataatga
     5101 gcagttgtaa agagtattga gaaagtcaga agatttaata ttaaatcctc tggctttttg
     5161 aaatattagg ataaaaattt aaataatgta tggaaataaa caatattaag gagagtggtt
     5221 tttgtggagg aattatggaa aaaggcaatt gaatttcatg gtcatgaatg tccagggtta
     5281 gctataggat ttaaagcagc acttgcagca aaaaaatatt taggatgtaa acagtcagga
     5341 gatgaggaaa ttgtatgtat ttctgaaaat gatgcttgtg gaatagatgg tatacaagta
     5401 gtgctaagtt gcacaatggg taagggaaat ttactactta ggatgactgg aaaatcagta
     5461 tatcatttct ttaatagaga aaatggaaaa tctataagga tatatgtaaa gtcaaaacca
     5521 tttgagatgg atagagaagg atacaaaaga tatttattag aaagtgattt tgaagaaata
     5581 tttgagatta cagatactaa gttaagactt cctgaaaaag caagaatatt taaatccctt
     5641 aaatgtgaag tgtgtggtga atttgcatct gagcaatata taaggattga agaaggaaag
     5701 aaagtatgtc ttgattgttt tgataaatat gaaagatttt atgaagagta ttaaaattat
     5761 aaattagatt aaagttaaaa gtattagata tgtgctaaca gtattttaag atgaattagg
     5821 tagcaacata ataaagtata taaataatat taattgaatc atacataatt aaaaagaggc
     5881 tgattagtat ttataaaata aaatcagtct ctttatgaat aacgaattat tattctggtt
     5941 gaggtgcatc tacaggacaa gctcctgcac aagaaccaca gtctatacaa gaaccagcat
     6001 ctataacata tgcgtcgtct ccagctgata tacagctaac agggcattca gcctcacaag
     6061 caccacagct tatacaagcg tcagtaattt tatatgccat tttgaaatcc tcccttttat
     6121 atgtattgta attcaataac attataacaa atattattga atataacaat taaattaatt
     6181 ctacaacaaa aactttatta tgatattctt gttttaaagt tttacaataa agtttaatct
     6241 gcttatattt ttatttattt taaagaagtt tattttaaaa ataaa
//
LOCUS       DAGSRM010000141         5913 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.147, whole genome shotgun sequence.
ACCESSION   DAGSRM010000141 DAGSRM010000000
VERSION     DAGSRM010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5913
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.147"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(239..2071)
                     /locus_tag="MJE33_003444"
     CDS             complement(239..2071)
                     /locus_tag="MJE33_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9649353.1"
                     /translation="MSRMGRGPMGKSMGAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLFLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEVQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVK
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            complement(2064..4310)
                     /locus_tag="MJE33_003445"
     CDS             complement(2064..4310)
                     /locus_tag="MJE33_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9649354.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELNKITLFMDKSSKDKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNVIFGKAILIVSGLEGDSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKEDKKGLVEFKNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            complement(4378..4839)
                     /locus_tag="MJE33_003446"
     CDS             complement(4378..4839)
                     /locus_tag="MJE33_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBY9649355.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEREFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
     gene            complement(5144..5710)
                     /locus_tag="MJE33_003447"
     CDS             complement(5144..5710)
                     /locus_tag="MJE33_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HBY9649356.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
BASE COUNT         1886 a         1090 c          637 g         2300 t
ORIGIN      
        1 gataatcacc tccaacattt tccttactac atattataat aaaataaaca ctttgtaaag
       61 acataaaatt atgtggctat ctcaaaatat agagaatcct ctttttcatt gttaaaatga
      121 taaaaataca ctttatttca tacacaaaag agtgccttat gaattaaaaa gttcatttta
      181 aggcaccctc atttatttta tcttattaat tacatgtcta tgctaagata gttttttgtt
      241 acattgcttc atcttcttca aattgagaat tgtaaagatt ggcatagaat ccgcctttct
      301 ttagaagttc ctcatgattt ccttgttcaa ctatatctcc ttcattcatt acaagaatca
      361 tatcagcatc acgtatagtt gataatctgt gggctataac aaaacttgtt ctaccttcca
      421 taagattatc catagctttt tgaatcaata cttctgttct agtatcgaca gaacttgtag
      481 cttcatctaa tattaaaatt ttaggatcag ctaaaatagc acgagctata gtcaataatt
      541 gtttttgacc ttgagaaaca ttacttgctt cttcatttaa tttcatctta tatccatctg
      601 gtaatgtctt aataaatcta tgaacatgtg cagatttagc agcctctata acttcttcat
      661 ctgtagcatt caacttacca tatcttatat tttccataat tgaaccacta aataaccatg
      721 tatcttgaag aaccatacca aacatttctc taagctcact acgattaaaa tctttaatat
      781 tatgaccatc aatcagtatt gaaccgctat taacatcata aaatctcatt aacagtttaa
      841 ctattgtagt tttacctgct cctgtaggtc caacaatagc aactttttga ccaggtttaa
      901 catttactga gaaatcattt attatagtct tgtttgggtt atatccaaag ttgacatttt
      961 caaaatcaat tttaccttct aaaccatcta ttgaaactgc attttcaaca atttggactt
     1021 cttcttcctc ttctaaaaac tcaaatactc tttcagaagc tgctgctgtt gattgcaata
     1081 aattggcaac ctgtgcaatc tgagtcattg gctgtgtaaa gtttcttaca tattgtataa
     1141 atgactgtat gtcaccaacc tcaattgttt tcttaattgc aagccaacct cctaagattg
     1201 ataccattac atatcccaag tttccaataa acatcataag tggttgcatt atacctgata
     1261 aaaattgaga tttccacgct gaattataca ggctatcatt tagtttatca aactcttcaa
     1321 ttacttcatc ctcacgatta aatgctttta ctattgtttg gccactgtaa gtttcctcaa
     1381 cttgaccatt tacatttcct aaatattctt gttgatatct aaagtatttt tgtgatttct
     1441 ttattattct tgaaattaca aacattgata ttggtaatat taaaactgcc actaatgtca
     1501 taattccact tatagataac atcattatca aaacacctat catagttgtt actgatgtta
     1561 taagctgtgt catactttga tttaaacttt gatttaaagt atctatatca ttcgtaattc
     1621 tcgataatat ttcaccatgc gtcttagtat caaaatactt cattggcaat ctatccaatt
     1681 ttgctgaaat ctcttttctt aaattatagg atactttttg agaaattcca ctcattataa
     1741 atccttgtat aaaagagaac agtgcactta tcaaataaag gaaaagtaaa aaggttaaga
     1801 tttttccaat tgcattaaaa tctataccta cgtttcctcc agaaacttta ctcactagcc
     1861 cttcaaatat ctttgtagtt gcctttccta aaatttttgg acctactata gaaaacgacg
     1921 cactaccaat agcaaataca attactagaa ttattgatat tttaaattta gataaatatg
     1981 caattagttt tcgcatagtc cctttaaagt catttgcttt ttgtcctgct cccatagatt
     2041 ttcccatagg acctcttccc attctactca ttagctagct cctcctttga aagttgtgat
     2101 aaagctattt gctcataaac ttcgcaattt tttagaagct ctttatgtgt tcctattcca
     2161 actatttcgc cctcatttaa aacaattatc tgttctgcat ttaagatagt actaattctt
     2221 tgtgcaacta ttagaactgt actttcagca gtttttgatt ttaaagcctt acgaagagct
     2281 gcatcagttt ttaagtcaag tgctgaaaaa ctatcatcaa atatataaac ttctggattt
     2341 tttgctattg ctcttgcaat agaaagtctt tgtttttgac ctccagatac atttgttcca
     2401 ccttgagaaa tttcagtttt aaaagcttcc ggtttagaat ctataaattc tgtagcttga
     2461 gctatttcag ctgctgttct catttcttca tctgttgcgt cttttctacc atattttaag
     2521 ttggattcta tagtaccaga aaacaatact cccttttgag gtacatatcc aattttttcc
     2581 cttaaatcat gttgagaaac attttttata ttaactccac caagtaatat ttctccttca
     2641 gtaacatcaa agaaacgagg aattaaattt ataagagttg acttaccact accagtactt
     2701 cctataaagg cagttgtttc tcctggcttt gctgtaaaag atatatttgt taatataggt
     2761 tcttctgcgt ctggatacat gaacgaaaca tttttaaatt caacaagacc ctttttatct
     2821 tccttaaact cttgtgcctc tgatttttct gggtctttta tgcttatatc tgacactaat
     2881 acttcatcta tacgtgtagc tgaaacggat gctcttggaa gcataataga taccattgat
     2941 atcattaaga atgacattat tatttgcatt gtatattgta taaatgccat catatctcca
     3001 acttgcattg ctccagaatc aacactgtat gcacctctcc aaactataag aactgtgata
     3061 aggttcatta taagcatcat agcaggcatc atacatgtca taactctatt tacaaaaaga
     3121 tttgttttag ttaaattttt atttgcttta tcaaatcttt cttcttcatg tttttcagta
     3181 ctaaaagctc tgataactgg aataccagta attatttcac ggcttactaa gtttaactta
     3241 tcaacaagct tttgaaccag tttaaactta ggcataacca ctgagaataa cactacaatt
     3301 aatgataaga tagcaactac tgctactgct ataatccagg ccattgaagt atttgtgttt
     3361 aaaaccttta tcactccacc tatagccatt ataggtgcat agaatactat tctaagcaac
     3421 ataaccatca acatttgtat ctgttgaata tcatttgtac tacgagtaat taatgaagct
     3481 gtagaaaatt cattcatttc agtatttgaa aatgacataa cttttctaaa tactccactt
     3541 cttaaatttc tacctacact tgctgctact cttgctgcta aaaatcctac tgttattgta
     3601 gctaccatac taatcattgc tataccaagc attttagcac cagtaaataa tatataatta
     3661 ttttgagttt tttctgtatc aatacctatt ttctcatatt ctgaccttac atatgaaaca
     3721 gagctttgtg ttatcatact ctctggcata ctttcaaagc ctttgctcat ctctttagtc
     3781 aatgtatcta actgttcctt tggcattgct gataacaatt taaatatatc aacatctcca
     3841 ctttgagtag cttgtggtgg aagtttgctc attaactgag ctttcatagc ttttacctct
     3901 ttactatcgc cctcaagccc tgatactatt aaaatagctt taccaaaaat aacatttaaa
     3961 tcatctatag tgtctttgtc tttagtattt aactcataaa gagattcatc ttttagacct
     4021 gggtacttat ctttatactt aacataatcc tttttattca gcaaagtata attatctagt
     4081 actttatcct tactactttt atccataaat aaagtaattt tatttaattc actctctcta
     4141 atcacagatg gaacagagtt ttctactcca ccttgttgta tacctacatt tactatattg
     4201 gatgtgtatt ctggtaaaga aaggtcacat accgcttgaa ttaccaatag acagactatt
     4261 attactattg ataatgccga tttctttaaa tatttaatta atttaaacat acatttcttc
     4321 ccttctgtaa tattaattag tctaataaca ttaaattgaa aaacattatt ttaaacttta
     4381 aaaatatttt tcatcttcat gcttatgaca tcctgctttt aaattatctc gtacttgcat
     4441 tagaagtctt ctaagtacta ttttttcttc ttcactaaat cctaaaaaaa actctctttc
     4501 caattccttg ttaaattctt caactttatt acatatttct aaacctttgt ctgtcaaaaa
     4561 aatcctagat actctttggt ccttttcatc ttgttttctt tccacaagtc cagctttttc
     4621 catcctcttt atcataactg tagttgtaga tgcttttact ttccttttat ctgctaattc
     4681 tttttgactt tgcccatctt tcatctttaa agaaaaaagt atttggggtt gaccatgata
     4741 taagcctaat ttatcaaaat aaaaatgagt tcttataaaa tatattcgca ttaattgcaa
     4801 gaaaacatca tttaattttt cttcagaatt gctattcata cttgccctcc ttatttttta
     4861 tattacttag tcagctaagt attattttac tatactgcat gcattttttc aatatattta
     4921 taaaaaattc atataaattt catttattaa atatttttat atcatatagt tataatttta
     4981 ataaataata gtaagatttt taattagtca gctacttata ttttttattg caaagcttaa
     5041 aataaacctt ttcatatatt ttcaaataaa aaagttgtcc caaaataaat tttgaaacaa
     5101 catcatattc aacagtatct tgattatata acattatgat actctactta tcttcatatc
     5161 catttacaac aacgtctaac ttcccagttt caggtgatat aattagacca tgtacaataa
     5221 taccatctgg aattaaagga tgccctttta ttaaatcaat actatctttt acagattctt
     5281 ctgcagaatc gaatccatgt aaccattgtt ttgtatcaat tccagcacta cgaagtgtaa
     5341 gtattacatc attagatatt cctctatcta aaattttacc aagtaactta tccgtatcaa
     5401 cattacacat accacatcca tgatgcccta caaccataac ctcatctaca tcaaattcgt
     5461 aaattgcaac taaaacactt ctcataatac ttccaaaagg atgcattata gtagctcctg
     5521 cattttttat tagtttcaca tctccatttt tcaaattcat agccttaggc aaaagctcag
     5581 ttaatcttgt atccatacaa gataagacta ctattttttt atttggatgt tttgatgtca
     5641 catattgttc atattctttg ttctctacaa atgatttatt atattctaat atctcttcaa
     5701 gttttctcat atgtaatatc accctcttta aaaatttttt tattattata tcactattaa
     5761 agtacaatat atacacatat aatttaattt atatttatta aattatttta taagattatt
     5821 actctactat aaatcttgta tataacttgt atttataagt atttgtatat ttatttttta
     5881 taatctaaac taatattata tattttcatt att
//
LOCUS       DAGSRM010000142         5873 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.148, whole genome shotgun sequence.
ACCESSION   DAGSRM010000142 DAGSRM010000000
VERSION     DAGSRM010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5873
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.148"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(344..751)
                     /locus_tag="MJE33_003448"
     CDS             complement(344..751)
                     /locus_tag="MJE33_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649357.1"
                     /translation="MSKVAKATFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDISSKQGEKQALKFLNNVLNIIVGICIVVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTKILIKK"
     gene            complement(1225..2109)
                     /locus_tag="MJE33_003449"
     CDS             complement(1225..2109)
                     /locus_tag="MJE33_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBY9649358.1"
                     /translation="MDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVE
                     LAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDY
                     KKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPT
                     IDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEV
                     EQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAK
                     AIRKLRHPSKLKQLKHFY"
     gene            complement(2400..2876)
                     /locus_tag="MJE33_003450"
     CDS             complement(2400..2876)
                     /locus_tag="MJE33_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HBY9649359.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGILDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEEVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQVKDLEEHHHHDENCGCGCNHHHEN"
     gene            3142..4317
                     /locus_tag="MJE33_003451"
     CDS             3142..4317
                     /locus_tag="MJE33_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HBY9649360.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQNYDYNSMKNTLDNVSDNVNEQLKKLGESVKGSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     NDSDTNSSKNDTSSNNENNDSSQTNENNTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            complement(4398..5201)
                     /gene="proC"
                     /locus_tag="MJE33_003452"
     CDS             complement(4398..5201)
                     /gene="proC"
                     /locus_tag="MJE33_003452"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBY9649361.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQGTLDRI
                     DANFGINTTKDSKEVTRTSDIVIVAVKPDIYDDILEEIKDFIDDNKIIVTIAAGKSIK
                     DIESIIGEDKKIVRTMPNTPALVNEAMSSLSINKNINKEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKVLEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(5241..5825)
                     /locus_tag="MJE33_003453"
     CDS             complement(5241..5825)
                     /locus_tag="MJE33_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY9649362.1"
                     /translation="MNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLTSV
                     ATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLKEK
                     TMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYNSE
                     KNNHNWVNYTNKVVKILVVSIPNPFEKSDAVKEA"
BASE COUNT         1931 a         1026 c          683 g         2233 t
ORIGIN      
        1 gcattatccg ttaaggtcta cccacttcca ctaccaatag cttgtagtgt acttccctca
       61 agaggaatag tcgttgaagt ttctcctatt cggagtttac ctgctgattg cccattttaa
      121 agtacttagg atttaacctt ataccatcta accaattttt tctactttcg taactttcac
      181 acttaggttt atttcatcct tatgttttag tttgattagc tttaggggtt tccagcaatt
      241 caagtagtat tggataggtt ctatcctatc tctacatata ggtttcccta tatgctgact
      301 attttagcta ctaactcatg actaaagtca cgagtgtgcg tagctatttt ttaatcaata
      361 ttttagtaaa ttttacggta agtaaaaacc tctctccttc aaatcctata gcaaatatac
      421 ttacaagttg ctttgagaaa ataactccta atatagctac aactatgcat ataccaacta
      481 ttatatttaa aacattgttt aaaaacttta gtgcttgctt ttcaccttgt ttactactta
      541 tatcttgata cattggaata aaagtagtaa ctatagcagt acctattgct gcaaatataa
      601 tatttggtat attcatagct gttaaataac tctctgtata taagcctgta ccataaattg
      661 atgataatac taattctcta cccattccaa gaatcttaga gattatagta actatcatca
      721 aataaaaagt tgctttagct accttgctca ttttatcact ccacttctaa catacctctt
      781 tttaatcaag taaaatttag ctcattagat ataattctat caactaattt gccttgctta
      841 gccctatcaa aatataatta acacctattt cttaatatat cacaaactac attattcttc
      901 atattttttt atttctccaa taagttttac atttttccta tataaattgt atagcttcaa
      961 ttatattcaa agaaatggag ttgctacccg ataactttaa aagtaaaaaa cagattttat
     1021 tatcttttta aactatattc taacatattt tgtccatatt gagaaatttt actaaactat
     1081 tatgtacttt attaaataaa taaaataaca gcacacctat aaaatacaag tgtgctgttc
     1141 ataaacagat tttataaaaa aataattcct aagtagagat taacatgctt tctcatactc
     1201 tcatatataa tgtgtctaaa aatattaata aaaatgcttt aattgtttta acttacttgg
     1261 atgtcttaat tttcttattg ctttagcttc tatctgtctt attcgttctc tggtaactcc
     1321 aaataccttt ccaatctgtt ctagtgtttt agggtcatca tcatcaattc caaatctcat
     1381 tcttaaaact tgctgttctt gctctccaag acctgtaagt agttcttcta tcttttcttt
     1441 taaattatgc tgttcaactt cgtgtattac aacatcttta aaatcagcat ctgatggtat
     1501 aaattcaact aaactactat cctcatcttc ttttaatgga gtatccaaag atacaacatt
     1561 tttatctctt cttaataatt ccaacacttt gtcaacttct aagccacatg catctgcaat
     1621 ctcatctata gttggctctc ttaataatac atttaaaagt tcttgtttct ttttctttac
     1681 aaaatttatt ctctgatgaa gatgtatagg tatcctaata gtattttcgc aatcgtcgat
     1741 atatcttgtt atactttgtt ttatccacca agttgcataa gtgctaaatt tgtatccttt
     1801 tttataatcg tacttttcta cagcttttat aagtcctata tttcctgctt gaattaaatc
     1861 caacatatca atactatctc ttttatatct ctttgctatg ctcactacca acctataatt
     1921 tgaatttata aatttttctt ttgctactga agatgagttg attatttcct tagccagctc
     1981 tacctcttct cctgctgaca acattttgta ttcctctatt tcctttaaat acattttcat
     2041 cgcattcgat acatttgatg aacccacaca aatataattt tcataattat tttctttttt
     2101 agtatccata atcaaacccc ttctattcat agaatttgct aaaacaaaaa taaataattt
     2161 aaacctacat aatctacaat tacataatat cccattcttt aaatgaattg ttttgtatat
     2221 tatgtataca aatttataaa aactatacct ataatattat tctacttcta acagcaatat
     2281 cctccttata tatataattt attttattat atgttgttat attcttagtt ttttgaatga
     2341 tatatgtcta aaaaaagact gtttactaaa cagtcttttt ttatatatat gctcaatatt
     2401 taattttcat gatgatgatt acatccacat ccacaatttt catcatggtg atgatgttct
     2461 tctaaatctt ttacctgttg ttccatttca tgaaactctt ttaccattat atcataagtt
     2521 ggaagagctg cctcagtatc gtattcttcc cctctccaat cagcgtacat catagcatct
     2581 ccattagtta aaaccaatct acctgacata gttatattat ccacttcttc taaaaattct
     2641 atttgttttt gtaaaacttc tccttctaac tccacatctt catctaaaca ttgcactatc
     2701 attacaggtg cataataatc ttcttcatct ttaacattta caacaatatc taaaattcct
     2761 tctgcacctt cttcaaattc tgccaactca acatttgtat caatcatatc ttgaggtact
     2821 aataaatctt ctattaaata ttttattact ctatctttta ctaattcttt tgacatttat
     2881 ttttctcctt ccgattctat aacattatca taatcatttc tatattatag tatactacat
     2941 taattgtttt ttcaatctct tacaaaaatt attttttatt atcttcctta tattcccttt
     3001 tttattacaa agttgtaatc gcattgattt aaaaaatagt ttatatttag tataatagaa
     3061 atgtagcaca tatttttatt caatatatta ataaaaaaat atattgaaaa tgtaatgtat
     3121 gttaaatcta ggaggaatat tatgaagttt aaaaaacaaa ttagttctat tgttgtgtct
     3181 actatgcttg tacttgggag tatgaacctt tcttatgcag acggtacaaa tgtagttaca
     3241 cttggagcta acttatcaga gtctcaaaag cagcaaatgc ttaattactt tggagtaaaa
     3301 aaagaccaag tcttagtttt agatgtaaca aatgcagaag aaagacagta tttacaaggt
     3361 gttgcaacag agggtcaact tggaaaagtt actatctctt gctcatacgt tgaacctact
     3421 aaaaaaggaa atggaattaa tgttaaaaca gcaaacttaa cttgggtaac tagttcaatg
     3481 attgcttcaa cccttacaac agctggtctt gaagatgcaa atgttattgc tgctgctcca
     3541 tatcctgtaa gtggtactgg agcattgaca ggaatcatga aagcatttga ggatgcttca
     3601 ggaaagaaac ttgatgaaac taagaaagaa attgcttctg aagaattggt agttacaggt
     3661 gacttaggag atgatatagg gcaagaaaaa gcaacaggtg taatgaatga tataaaaaca
     3721 gaaattgtaa agaatggtac aaaagatacc aatcaaatag caaatacaat aaataatgtt
     3781 gtaaataatt acaatattac tattacacca gaacaaaaag aacaaattaa aggtgtaatg
     3841 aaaaaaatag ctgaccaaaa ttacgattac aatagcatga aaaatacact agataatgta
     3901 tcagataatg taaatgaaca attaaagaaa ttaggtgaaa gtgttaaggg ttctggtata
     3961 ctagatacta ttggtggatg gtttagtgga ataggagact ggttctctgg tttattctca
     4021 ggcggagata aaaaagattt gggaatctta aataatacta atgatgagtc tcttggctct
     4081 gatgctgtaa taaattctac agaaggtgtt caaataaatc cacaaaataa tactaattct
     4141 aacaatgata gtgatacaaa tagctcaaaa aatgatactt caagtaataa tgaaaataac
     4201 gatagttctc aaacaaatga aaacaacaca acaaataatt cagaaaatca atcaaataat
     4261 aatgactcag ttggtttctt tgaaaaaata aaaaattggc tttcaggctt attttagaaa
     4321 ttaaacaaat aaaaagaatt gcctaaaatg gaaattttct ataaaaggca attctcttta
     4381 tttacaataa atttttatta ctcactcatt tttttagatt tttctataca tgcacatatt
     4441 gcttttatca cagaagctct aaaaccttct tcttcaagta ctttaactgc ttctatagta
     4501 gttccaccag gtgaacatac catatctttt aattcaccag gatgttttcc tgtctcaagt
     4561 accatttttg cagaacccat aacagcctgt gatgcaaact tatatgcttg tggtcttggc
     4621 attcctgcaa ttactgctgc atctgctata gcttctataa ataaaaatac atatgctggt
     4681 gcagaaccac ttgcaccaat tacagcttct ataagatact ctggtactac ctcagtattt
     4741 ccaaaagaat taaatacttc tgtaactgct tctaagtctt ctttgtttat atttttattt
     4801 atcgaaagag aagacatggc ttcatttaca agagctggag tatttggcat agttctaact
     4861 atttttttat cttctcctat aatagactct atatctttta tagattttcc tgctgctatt
     4921 gtaactataa ttttattgtc atctataaag tcttttatct cttccaaaat atcatcatat
     4981 atatctggtt taacagctac aattactata tcagaggttc tagttacttc cttcgagtct
     5041 tttgtagtat ttattccaaa atttgcatct attctatcga gagttccttg actatatgct
     5101 gatgctataa ccatattagg ttcaactaat tttgaattta ctattccccc tatcatagcg
     5161 cttgccatat ttcctgcacc tatgaatcca atttttttca ctttagtacc tccttattga
     5221 ctaatagtaa tcgttaaaat ttaagcttct ttaactgcat cagatttttc aaatggattt
     5281 ggtatactta ctaccaaaat tttcactacc ttattagtat agttgaccca attatgattg
     5341 ttcttttcac tattatagtg tattgtgtct cctggataca tttctatttg ttcatctccc
     5401 aaaaacactg taagagtacc ctctaataca tacacaaatt cctcaccttc atgtacgtaa
     5461 caacaaattt cctcactact cttacttgga agaatttcaa ttaatcttgg tagcattgtt
     5521 ttttccttta aactagatga taaatgataa tgtataaatg ttgagttttc tacatcaaat
     5581 acttcttttt cataacttct taatacagct cttttatgct cttttggctt cataaaaaag
     5641 taagttaatt ctacatctag tacacttgct atttttccta aggaatctgt tgccacactt
     5701 gttaatcctc tttcaagttg tgataaaaaa ccaattgaca agtttgtttt ttcactcata
     5761 tccttcagcg tcatttgctt ttgagttctg agttgcttta tttttgctcc tatgtcctta
     5821 ttcaaaacca tcacccctaa ataattttaa ttttttcata atataaatac aaa
//
LOCUS       DAGSRM010000143         5742 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.149, whole genome shotgun sequence.
ACCESSION   DAGSRM010000143 DAGSRM010000000
VERSION     DAGSRM010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5742
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.149"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            407..913
                     /locus_tag="MJE33_003454"
     CDS             407..913
                     /locus_tag="MJE33_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HBY9649363.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTIAFFDETYREALNICGTISGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHGNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKVLVKE"
     gene            1326..1994
                     /locus_tag="MJE33_003455"
     CDS             1326..1994
                     /locus_tag="MJE33_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649364.1"
                     /translation="MENITSYFTTILCVFICLSSVFIFTQLARVFINKKKINQKIKSR
                     NGFRYDRDFIEARREEIHIKDNNNNKNKSNNKKLKEEKVFKYDNGDLYKGEFVDGKKN
                     GFGIYIFSGKEKYEGLWKDDKMHGIGKYTYRDGSIYTGEFKYGLKNGLGKLTYPNNDI
                     YKGYFLDNKKNGKGVLYKNDGNKQAGIWEDDEQCKSLDFKDLNNNKINNVYQNRLNNN
                     KLKV"
     gene            2153..2659
                     /locus_tag="MJE33_003456"
     CDS             2153..2659
                     /locus_tag="MJE33_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HBY9649365.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGITEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGDRTIIAHNAKFDLKFLNYYL
                     RMLDLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     FLKIKDEY"
     gene            2787..3803
                     /locus_tag="MJE33_003457"
     CDS             2787..3803
                     /locus_tag="MJE33_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBY9649366.1"
                     /translation="MGYYNDNDNNKIRRKKISNNKDSNKKSKHSYTSANKKNKSPNTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPNDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGASTIDMQVSKNLLTS
                     NDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKNAYGAAKGAEVYFGKKVSE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQVIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     gene            4157..4861
                     /locus_tag="MJE33_003458"
     CDS             4157..4861
                     /locus_tag="MJE33_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HBY9649367.1"
                     /translation="MKKFVIEDAFWNLFPSAKIGVVICNDIDNSIKDEDYKKIILKGE
                     EEALKYLEDSEFSNNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENSFPYEGEIIYKDD
                     GGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALNELAKIVQEKLGGN
                     CKISILDIDNKEVSVD"
     gene            5156..5635
                     /locus_tag="MJE33_003459"
     CDS             5156..5635
                     /locus_tag="MJE33_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBY9649368.1"
                     /translation="MSNKLIKNLLSGILQILFFLLGLVIVVGGFKSFMYLCFSGEATL
                     QGTIYGILMFILGVSYFIIIKSLIEVLGSSEYSLFVKENVKRFRIIGYLLLLNSLIEF
                     ISTFGTTGKGMRFLDLGFGFYFTVPVFVYFITSLMSFVIADGFVKAIKIKEDNDLTI"
BASE COUNT         2405 a          486 c          937 g         1914 t
ORIGIN      
        1 aaatgtaata acttaaatgt tgttaataag atataagaaa ttataaataa tagagtagaa
       61 cttgacaatt ataacaattt tttataaaac tgaatcaaga taaaattaaa ttaatataaa
      121 aataatatac aataaaaaat gtagagagat acttactttc tacatttttt tattgtatat
      181 tgctttaggt ttaaaaatat attatatact ttataatcaa tatattttat ctatatttta
      241 cacttataaa atatgaaaac tacttattgc gaaagtaatt gatttgttgt attataattt
      301 acatgaatta tgtaggacaa tcaaaagtca gtattataag agtacatata ttcaaatttt
      361 taacaattaa tttatataat taaaaaatga agggtaggat gaagtcatgg gttttagaga
      421 agtaaaaatt gaggaattac agtttaatcc atttactaag attggcaaag aatggttact
      481 tataacagct ggtgattcag aaaagtttaa cacaatgact gctagctggg gtggtgttgg
      541 agtctattgg ggtaaaaatg ttgttacaac ttacataaga ccacagagat atacaaaaga
      601 atttgtagat agtaatgata catttacaat tgccttcttt gatgaaacgt atagagaagc
      661 tttaaatatt tgtggcacta tttctggtag agatataaat aaaatagaaa aagcaggact
      721 tacaccatat tttgtggatg atacggtagc ttttgaagaa gctaatatga ttatagtatg
      781 taaaaagcta tatcatggta atatgccacc agaaaacttt gatgctaaag aaaatgataa
      841 aaaatggtat ccagagaaag actatcatac tatgtacatt tcagaaatta taaaagttct
      901 tgtaaaagaa taattataat aaaaaatata tgaaaagaac ttattataag taaatgactg
      961 tgctttctta tctgatatct aagtagatgc tactaactaa ataaatatta attttaaata
     1021 aatactatta taaaaaagat gaactatatt tactatagtt catcttttta tttatataag
     1081 cacttatata agtagagatt tatttgaata aaagtattta tttgaaatat ttgagtatac
     1141 tttataagta tttttatgct aactagaaac acttaattta tggcatttgt acttgattta
     1201 ttttaatgta aataatttca aagttaattt tattcaatat agtactataa aagtataaat
     1261 aacatttaat aaaaatatat attatattag gtattttaaa taaatttttt aaagggtgat
     1321 tttttatgga aaatataact agttatttta caacaatact atgtgtattt atatgcttat
     1381 catctgtatt tatatttact caactagcta gagtatttat taataagaag aagattaatc
     1441 aaaaaattaa atcaagaaac ggatttaggt atgatagaga ttttattgaa gccagaagag
     1501 aagaaataca tataaaggat aacaataata ataaaaataa gagtaacaat aagaaattaa
     1561 aggaagaaaa agtatttaaa tacgataatg gtgatttata taaaggtgag tttgttgatg
     1621 gaaagaaaaa tggatttgga atatatattt tttcaggtaa ggaaaagtat gaaggattat
     1681 ggaaagatga caagatgcat ggaataggaa aatatactta tagagatggt tcaatatata
     1741 caggtgaatt taaatacgga ttaaaaaatg gtttgggaaa attaacatat cccaataatg
     1801 acatttataa gggatatttt ttagataata agaaaaatgg taagggtgta ttatataaaa
     1861 atgatggaaa taaacaagca ggaatatggg aagatgatga acaatgtaaa agtttagatt
     1921 ttaaagattt aaataataat aaaataaata atgtttatca aaatagatta aataacaata
     1981 aattaaaagt ttaattattc taataaaaaa atctttattt atgatggaat ataggatata
     2041 tctatgttgt aatttgtaaa atcttcctat atcatataaa attaagtatt gattagtcta
     2101 ttacataaat atagaatata aatatatcca tttaataata aggagaagag atatgaaaga
     2161 gtatgtagtt gtggatttag aaactacagg attagatcca tataaaggtt gtgaaataat
     2221 agaaatagga ataacagaaa taaaaaatga acaaattgta aaaaactatt caaggttgat
     2281 aaaacctaag gggataattt cttcatttat aacagaattg acacatataa gtaatgaaat
     2341 ggtagaaaat gaagaaccac tagaattagt tttacctaga tttagaaaat acataggtga
     2401 tagaactata attgcacaca atgcgaaatt tgatttaaaa tttttaaatt actatcttag
     2461 aatgttagat ttagaaccaa taaacaatta tatatgtaca gtagagttat taaaaaaatg
     2521 taaaagttat aaaggaaaaa acaagaaact tgaaacagct tgtgcttatt acaacataga
     2581 gaatataaat gctcatagag ctgacagtga tacattagca actgcaaaac tatttttgaa
     2641 aataaaagat gaatattaat tataaatata gagaaaatta gtgaaaatat cctttataaa
     2701 attgaccctt aagcttacaa ttttgttctt tgatttattt tgtagaacaa tgtataatgt
     2761 accttatatg aagaagggag actaaaatgg gttattacaa tgataatgat aataataaaa
     2821 taagacgtaa aaaaattagt aacaacaaag atagtaataa aaaatctaaa cattcttata
     2881 cttcggcaaa taaaaaaaat aaaagtccta atacaagttc cagtaaaaga atgagtgaaa
     2941 aagtagattc acagaatctt ataaaaagag atagagatag ttttacaaga gataatgtaa
     3001 gacctttaaa tacagattac aatcaaagag aaagaagaag agtcgagtat aaacaaagag
     3061 agaaaaaatt aaaaagtaaa aaaagaaaaa aacaaaaaag gattagaata gttagggcat
     3121 tattatctat tatgttagtt gcactaatta tattttcagt ggcaggtgca atatttacag
     3181 tttcagctat aaaagggtca ccagaagtta ctaaaaagtt aattaggtct aaatatataa
     3241 gtagtgaggt tgtaagtata aaacaaatgc caaatgattt aaaacatgca atagtttcca
     3301 tagaagatga gagattttat aagcataaag gagtagatgt tatttctcta gttaggtcag
     3361 tattaaacaa tgtgtttaca gatacaacac aaggtgcaag tactatagat atgcaagttt
     3421 caaaaaacct gcttactagt aatgataaaa ctatgaagcg gaaaataagg gatatgtata
     3481 atgctaagca gatgaataag attatgacta aggatgaaat tcttgaagca tatttaaata
     3541 atatgtatct aggtaaaaat gcatatggag ctgcaaaggg agctgaagtc tactttggta
     3601 aaaaagttag tgaattaaat ttagctgagt gtgctatgtt agctggtatg acaaataatc
     3661 ctgcaaggta tattgaccat ggagaggcta agaagagaca agtgattgta ctgtataaga
     3721 tgcatcagct tggttatata actgataagg agtatagaat agcaaaagct gatgacactc
     3781 catttaaatc agaaattgat taaaattgta agaatatagt attttcaata gtttaaacaa
     3841 acaaaaatgt aataaaaaag gtgatttgcc acccatttgc caccataatt ataatctaat
     3901 ggcaaattgt tttaatgtac aaactagata atagaatatt gtcttaaatg ctatattatg
     3961 ctaaaattta attgtatttt gtaaattaaa aaacttataa tttagttttc tagggttccg
     4021 cattttataa tggtcaggtc caagagaaaa cgcgcaattg ttgtgtacac ggaaggataa
     4081 aagcctggga gatatttaag aatatttcta taggcttttt tatttttaca tattttaaat
     4141 ttgaggaggt tttctaatga aaaaatttgt tattgaggat gcattttgga atctatttcc
     4201 tagtgcaaaa attggagttg ttatttgtaa tgatatagat aactctataa aagatgaaga
     4261 ctataagaaa ataattttga agggagaaga agaagcatta aaatacttgg aggactcaga
     4321 atttagcaac aaccaagtga ttaaagtatg gagagaagca tttaaaaaat ttaaaacgaa
     4381 aaaaggagca aggtcttcta ttgaagcatt attaaaaaga gttcataatg gaaataatat
     4441 aggaactatt aaccctttag tggatatcta caattttatc tcattaaaat atgcattacc
     4501 ttgtggaggg gaggatatag ataaatttat aggagatatt aggctaacta gagcaattgg
     4561 taatgaggag tttatcccac tagggacaga tgaaaattca tttccatatg aaggagaaat
     4621 aatttataaa gatgatggtg gagcaatttg tagatgttgg aattggcgtg aagcagtgag
     4681 aacaatgctt actgaagata ctaacaatgc ctttttatgc attgaattag tagatgaaag
     4741 tagatttaaa gaatttgaaa atgctttaaa tgaattagct aaaatagttc aagaaaaatt
     4801 gggtgggaat tgtaaaattt caattttaga tattgataat aaagaggtat cagtggatta
     4861 atatggaggt gctgttgtaa tggactattc tagttataaa gatacttata tttagggtaa
     4921 gaaggtctat tttagatata acgattatca aaggatataa tatagaaaat aaaaatgtag
     4981 aatataaaaa gcctttaaaa taagaaaaaa atgtatttta aaggctttta ttatactctt
     5041 ttttatttgc ataattaaca ataaaaatat attgttaatt ataatatttt tatttaaaaa
     5101 cgatataata taatgtatta tactatatat aaaatatttt aaaggagttg gcatgatgtc
     5161 taataaactt ataaaaaatt tactaagtgg tatattacaa attttatttt ttcttttagg
     5221 attagtaatt gttgtaggag gatttaaaag ttttatgtac ctctgtttta gtggagaagc
     5281 aactctacaa ggtactattt atggaatatt aatgtttatt ttaggtgtat catattttat
     5341 cattataaaa tcattgatag aagtattagg tagttctgaa tatagcctat ttgtaaaaga
     5401 gaatgtaaaa agatttagga ttattggata tcttttgctt ttaaactctc taattgagtt
     5461 tatttcaact tttggtacta ctggaaaagg aatgagattt ctagatttag gatttggatt
     5521 ttattttact gttccagtat ttgtatattt tataacttct ctgatgagtt ttgtaattgc
     5581 agatggattt gtaaaggcaa taaagataaa agaggataat gatttaacta tttaggtgtg
     5641 acactttaat atataggaga taaataaccc gaaaagagtg actaactagt aatttaaaat
     5701 aaaatatatt agggtataat acataaacta taatgctaaa at
//
LOCUS       DAGSRM010000144         5577 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.150, whole genome shotgun sequence.
ACCESSION   DAGSRM010000144 DAGSRM010000000
VERSION     DAGSRM010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.150"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            240..1067
                     /locus_tag="MJE33_003460"
     CDS             240..1067
                     /locus_tag="MJE33_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBY9649369.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPLMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGNGTVWLAPTQVMYEKLSFGAMPTNDNMNNKVSKQK"
     gene            2092..2556
                     /locus_tag="MJE33_003461"
     CDS             2092..2556
                     /locus_tag="MJE33_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY9649370.1"
                     /translation="MNLNKVKFTEEHAIEVTNWKYEGKYSIYNLPPWEEIKKKNFSLA
                     KEDKRKNFISFINDNKELIGFINLLDEGSSVFFGIGMKPNYCGMGIGKEIIGLGLQEC
                     RNKYSTKPIVLNVRTWNMRAVKCYESQGFKIVETKVQKTHLGDGEFFVMRYQ"
     gene            complement(2823..3467)
                     /locus_tag="MJE33_003462"
     CDS             complement(2823..3467)
                     /locus_tag="MJE33_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9649371.1"
                     /translation="MFGQSFQNYLVLIKENFKLSTLTNLICSIILLCMIPLIQGVANL
                     DRISTAICLENYVAIIGIVMLVPVFSLEQSKEIDEIVSSKALSQVIVQLIRILMSIIG
                     IVVLIIGFVNVLIYLGCNFPVVEYIIGSFASAMFIGSLGMFFSNLFNNTIIGYMISIG
                     YLILNMMTKGKYVGKFFIMSMSQGSFDEKYWLILGSVVLIIASLLIKPVKRKIR"
     gene            complement(3397..4686)
                     /locus_tag="MJE33_003463"
     CDS             complement(3397..4686)
                     /locus_tag="MJE33_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9649372.1"
                     /translation="MNLFIKECKNILKSMIYYIFIVVLVFFYISQLGSSVSNDVSKNI
                     PNDGNPLVAPQKVENIEEYYSVHNQYPYGQKESMKTEKVIPNATINLIREYQKNTYTT
                     YPTGVVKYIKLDQNKTKTIEKYIEEITGKSISEINSLWKTDESIKIDYSKVSLERFEE
                     IMQRVDDILGGGSAYAFDKLQRFGVVPVTYDEAMKNYNYTIQKDKVSNGYARIFCDYI
                     GIVLSIFPVFVAVFMSLKDKRSRMREIIYSKQVSSCKIVLSRYFALVFMMILPVLILG
                     MKDLLPLIFFAKDKGISIDNLAFIKYTLWWILPTLMIVTAVGMSITILTDTPVAIIVQ
                     LIWWFYSINSTGLKGDYSTMGLMIRHNSVLNGDIIIQNFNQIMINRIVISLEALVLVL
                     LAIFLYEQKRRGNLDVWAKFSKLFSSNKRKLQIKHFN"
     gene            complement(4683..5555)
                     /locus_tag="MJE33_003464"
     CDS             complement(4683..5555)
                     /locus_tag="MJE33_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9649373.1"
                     /translation="MEMKIAVSNLSKRFGKKQALDNINLEIDSGMFGLLGRNGAGKTT
                     FMRILATLLSKTEGSIHMCGVPIENTKEIRNMVGYLPQDFSMYPNMTVYKAMDYLAVL
                     SDIPLRKRRKIIAKLLSKVNLTNYQNVKVKSLSGGMNRRLGIAQALIHNPRVLIVDEP
                     TAGLDPEERIRFRNLLCEIANDRIVILSTHIVGDIEATCENVAILNSGKVIYNGSTEA
                     LAKLAEGKVYSIEVDKKDIENIKSRFIVIGMLTHGGKAILRIISDDKPFETAVNCNPT
                     IEDGYMLIMGGDNI"
BASE COUNT         2021 a          770 c          697 g         2089 t
ORIGIN      
        1 ataattttaa catgcccata aaatataata cccacaaatg ataaataata ctcaaaatat
       61 ctattgaaat aactatacat ccttaaataa cttaacttaa atctaaacat ttcaaaaact
      121 tcttaaaaat atgtaaaaat ttgatgataa agtagcgatt ttttcaaaaa aaatgtaata
      181 tagagttata tcttataata ttttagagat aaattttatc atagtgggga gaaatttgta
      241 tggatttaag caaaaaagta aaaattttag aaaatgcatc agatggcaaa agcgaatttg
      301 agatattaga atttgaaaaa cttgagggag cttctagccc attaatggca atgggattat
      361 attttgctag agaagctggt ttgaaagcta gacaaattaa gataaaatta aataattcat
      421 caataaaaac agaagctggt gcattatact attataaagg aaatatatct tctgaaacta
      481 agattggtgg tgttggaggt ctttttaaaa aaggaatatc aggagcactt actaatgaat
      541 caatgatgaa acctgtatac aaaggtactg gagaagttta tttggaacct tcttttagac
      601 attatttatt tatgagtctt gataatgatt ctataattgt tgataaagga ttattctatt
      661 gttgctctga tacaatagaa gtaaaagcat ttacacaaaa gaacttctca tctgctttac
      721 ttggtggtga aggcttcttc caaatagaat taagtggtac tggtgtaatc gttctggaat
      781 gtgttgtacc tcaaagtgaa attgtagagt atgaattgaa aaatggtgaa gaattaaagg
      841 tagatggtaa ctttgcaata gcaagaacat ctggtgttaa tttttctgtt acaaaatcag
      901 ataaatcttt aattggttct gctttaaatg gtgaaggttt cttaaataca tttactggta
      961 atggtacagt ttggctagct ccaactcaag taatgtatga aaaactaagt tttggagcaa
     1021 tgcctactaa tgataatatg aataataaag tttctaagca aaaatagttg ttttataaat
     1081 atttattaaa tcattattga ttttataaag tcactataaa ttttattaga ttattattat
     1141 aaaatcagag tgtggaaatg tttatgtgta ttctttaaat aaaaatttcc acattctgta
     1201 aggttattat acttgatata taaaattcaa tttaggaaca tagtctaaca tttataaaca
     1261 tattaaggta atgatttctc ttagtaatta ttctcatatc ttattctttg attatattta
     1321 tcttattcct agcccttatg tcatattata tttcttatct tattatttat aattagcatt
     1381 aacttatcta aaatcttgta tttttttaat agtaaagttt acatctatat taaaaagttt
     1441 acatctatat taatttcata aaacaatcaa ggtagtcaag agattcaact ttatttgatt
     1501 ttaaaaattt aagtgaattt gttcttatac acaccttttt aaaattatat ctgtagaaaa
     1561 tatcatttag agctataatt aaattaactt attaaatttt agaaaattta ttaaaaaata
     1621 taagttaaat taatgttttt aaagattctt ttttgtaact actagaatac taatttagta
     1681 gttattgtat ctctaaagtt taagcatgga tagatataca tttgataatt tgcctataat
     1741 ttacctacta cttggattat tggagcaaat acaatatatt aataataagt tcctaaaacc
     1801 aataaattaa ctaatacaca taaagatatt gatgttggtt atacaactat aagaaacgga
     1861 tttaacagaa tttaatcaag ctaagaaatt atatatatat cgtgatattt ttgaattaga
     1921 aggtaattca aaattagttt atagtcttct gtgaatccaa taaaaagtac agggtacaag
     1981 aattgttagt aaaattttta aagattttat ggataaatat aaatagtttt actcattgtc
     2041 taaaacttta aaataccatt aattacctta aatattttaa taggaggaca tatgaattta
     2101 aataaagtta aatttactga agaacatgct attgaagtta ctaattggaa atatgaaggt
     2161 aaatattcaa tatacaatct acctccatgg gaggaaatta aaaagaagaa tttttcacta
     2221 gcaaaagaag ataaaagaaa aaattttata agttttataa atgacaataa agaattgata
     2281 ggttttataa acttattaga tgaaggaagc tctgtattct ttggtattgg tatgaaaccg
     2341 aattattgtg gaatgggaat tggtaaagaa ataattggcc taggattaca agaatgtaga
     2401 aataaatata gtactaaacc aattgtatta aatgtaagaa cctggaacat gagagctgta
     2461 aaatgttatg agtctcaagg atttaaaata gttgagacta aggtacagaa aacgcattta
     2521 ggagatggag aattttttgt tatgaggtat caatagttta taacaatagt cttattgtta
     2581 atacaacaat taaaatgaaa aaatacatat gtgtaggcca tagcaataag tgtctttgaa
     2641 atataaattt gagagctttt gataaaattt ggtatacata tatattttat actacggata
     2701 tataaatcta tctacatata taaagagatt gtaagtaggt ttgtgtatat aaaaatagat
     2761 ttatatttca taaatgtact ttttatttgt attaccttta ttttatctaa gctctcattt
     2821 tattatctaa tcttcctttt tactggtttt attaatagag atgcaattat caacacaaca
     2881 cttccaagta tcagccaata tttctcatca aaactacctt gactcataga cattataaaa
     2941 aattttccaa catacttgcc tttagtcatc atatttaaaa ttaaatatcc tatagatatc
     3001 atataaccta tgattgtatt attaaataaa tttgagaaaa acattcctaa acttcctata
     3061 aacatagctg atgcgaaact tccaataata tactcaacta ctggaaaatt acatccaaga
     3121 tatattaaaa catttacaaa acctatgatt aataccacta tacctataat tgacatcagt
     3181 atcctaatta attgtacaat aacttgagat aatgcttttg aagatactat ctcatcaatt
     3241 tcttttgatt gctctagcga aaatacaggt accaacatta ctatccctat tattgcaaca
     3301 taattttcta aacatatagc tgttgaaatt ctgtctaaat tagctactcc ttgtattaga
     3361 ggaatcatac atagcaatat aattgaacat attaaattag ttaaagtgct taatttgaag
     3421 ttttctttta ttagaactaa atagttttga aaactttgcc caaacatcaa gattccccct
     3481 tcttttttgc tcatataaga atatagctaa caaaacaagc accaatgctt ccaagcttat
     3541 cacaattcta ttaatcataa tttgattgaa gttttgtata ataatatctc catttaatac
     3601 actattatgt cttatcatta gtcccattgt tgaataatca cctttgagtc ctgtagaatt
     3661 tatactgtaa aaccaccaaa ttaattgcac aataattgct actggtgtat ctgttaatat
     3721 agtaatactc attcctacag ctgtaactat cataagagtt ggtagtatcc accataatgt
     3781 atattttata aatgctaagt tatcaataga tataccttta tctttagcaa agaaaatcaa
     3841 aggaagtaaa tctttcatac ctaaaattaa tacaggcaat atcatcataa agacaagtgc
     3901 aaaatatctt gataaaacta ttttgcatga agatacttgt tttgaataaa taatctctct
     3961 cattcttgaa cgcttatctt taagtgacat aaaaactgcc acaaatactg gaaatatact
     4021 taaaactata cctatataat cgcaaaaaat tcttgcatac ccattactaa ctttatcttt
     4081 ttgtattgta tagttgtaat tcttcatagc ttcatcatag gtaactggaa ctactccaaa
     4141 tctttgtaac ttgtcaaaag catatgcaga ccctcctcct aaaatgtcgt ctactctttg
     4201 cattatttct tcaaatcttt caagagatac tttagaataa tctattttta tactttcatc
     4261 agttttccaa agtgaattta tttcgctaat acttttacca gttatttctt ctatatattt
     4321 ttctatggtc ttcgttttat tttggtctaa ttttatatac ttaactaccc ctgttggata
     4381 cgttgtatat gtgttttttt gatattctct aattaggttt atagttgcat ttggaatgac
     4441 tttttctgtt ttcatagatt ctttttgacc atatggatat tgattgtgta cagagtaata
     4501 ttcctcaata ttctctactt tttgtggagc aactaatgga tttccatcat taggaatatt
     4561 tttacttaca tcatttgaaa ctgaacttcc aagttgagaa atatagaaaa aaactaaaac
     4621 tacaataaaa atataatata tcatactctt taatatattt ttacattctt ttataaatag
     4681 attcatatat tatctcctcc cattataagc atatagccat cttcaatagt tggattacaa
     4741 tttactgctg tctcaaaagg tttatcatct gatataatcc taagtatagc ctttccacca
     4801 tgagtcaaca ttcctataac tataaatcta ctttttatat tttcaatatc ttttttatct
     4861 acttcaatac tatatacttt cccttcagct agttttgcta atgcttctgt tgagccattg
     4921 tatatgactt ttccactatt caatatagct acgttttcac atgtagcttc tatatctcca
     4981 acaatatgtg tagataaaat aacgattcta tcatttgcaa tttcgcatag taaatttcta
     5041 aatcttatac gttcttctgg gtcaagacct gctgtcggct catctactat taaaactcta
     5101 ggattatgaa ttaatgcttg tgctatccca agccttctat tcatacctcc agataaagat
     5161 tttactttca cattttgata attagtaagg tttactttgc ttaataactt tgctataatt
     5221 tttcttcttt ttcttagtgg tatatccgat aaaactgcta aataatccat tgctttatat
     5281 actgtcatgt taggatacat tgaaaaatct tgtggcaaat aaccaaccat atttctaatt
     5341 tccttagtat tctcaatagg aactccacac atatgtattg aaccttctgt ttttgataat
     5401 aaagtggcta aaattctcat aaatgtggtt tttcctgctc catttcttcc taaaagacca
     5461 aacataccac tgtcgatttc aagatttata ttatcaagtg cttgtttttt cccaaatctt
     5521 ttgctaagat tggatactgc tattttcatt tccatatata aatacacccc cagaaac
//
LOCUS       DAGSRM010000145         5533 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.151, whole genome shotgun sequence.
ACCESSION   DAGSRM010000145 DAGSRM010000000
VERSION     DAGSRM010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5533
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.151"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            55..171
                     /gene="rrf"
                     /locus_tag="MJE33_003465"
     rRNA            55..171
                     /gene="rrf"
                     /locus_tag="MJE33_003465"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(385..1569)
                     /locus_tag="MJE33_003466"
     CDS             complement(385..1569)
                     /locus_tag="MJE33_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBY9649374.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMESNGVKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCNYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMVKLVNAVPVFIDTKKENGFKLTKDELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYTGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKTIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSIDIDNMVKTYKSRRDLIKSKLDSIDNVGYVNPNGAFYIFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLDKLKEFVYKI
                     MA"
     gene            1942..4293
                     /locus_tag="MJE33_003467"
     CDS             1942..4293
                     /locus_tag="MJE33_003467"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HBY9649375.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKEKLIQTFDIDDELVNKMQNFAFEESYRETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERLEVQGNKKMKNFPFLMGQGVWKGSDDLGPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRHKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            4315..4854
                     /gene="nrdG"
                     /locus_tag="MJE33_003468"
     CDS             4315..4854
                     /gene="nrdG"
                     /locus_tag="MJE33_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBY9649376.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEEIAREAKINGLDVWSYTGF
                     TFEQLLDKKNRAYFKNLNLLKQIDILVDGKFIAEKKDISLKFRGSSNQRIIDVQKSLK
                     YKKVFLVEQYMKDDLSIAE"
BASE COUNT         2156 a          628 c          891 g         1858 t
ORIGIN      
        1 ctaggttcta taaacttatt aaaaacttaa tagaaatatt aagcataaag attatgtggt
       61 tacaatagca gagaggatac acctgttccc attccgaaca cagaagttaa gctctctagc
      121 gctgatggta cttggtggga aactgcctgg gagagtagga cgtagccacg taattttttt
      181 gttataataa atttagaatt taacttatat agtttaattt gataatttga tgaaaaaaat
      241 aaaagactta ctgattttta aatctatagg tcttttattt tttatctaac atttaaatta
      301 agttgttgaa tatatcttat attacttacc ttatatatta attaccatta atacattata
      361 tctaattact attcatctaa attattatgc cattatttta tatacaaatt cttttaattt
      421 atctaatcca gataagaaag tattttcact gcaagcatag gatatacgaa tatatttatc
      481 cataccaaat gctatgccag gaacaactgc aacattatat tcttctaaaa attgattaca
      541 aaattctata gaaaaactat ctttatattc aaatttctca gatactttag aaagatctat
      601 aaatatatag aatgcgccat ttggattcac atatcctaca ttatcaatag agtctagttt
      661 tgatttaatt aaatctcttc ttgatttata tgttttaacc atattgtcta tgtcaattga
      721 gcaatctttc aaggctccat aggctatgta ttgtgctgtt agacttgggt gagatataag
      781 atgtccttgt atagaactca ttgctttagc aatcgtttta ttagatgcag tatatcctaa
      841 tcttaatcca gtcattgcag ctgacttaga aaaaccattt atagttattg ttatatcttt
      901 agcttcttcg cttaatgatg caattgatgt aaattcacca gtgtagcata ttttttcata
      961 tatttcatct gctaaaatat atattttatt ttgtatgcat acatctacta tttctattaa
     1021 ttcatctttt gtataaacac taccagtagg atttgaagga ttgtttatta caagaatttt
     1081 tgttttatct gttatagatt tttcaagttc atcttttgtt aacttaaatc cattttcttt
     1141 tttagtatca ataaaaactg gaacagcatt aactaactta accatttcag gataactaac
     1201 ccaataaggc tttggtaata agacttcatc tccttcgtct gttaaagcta gtaaagtatt
     1261 tgttattgag ttttttgctc cacttgatac aactatttca tctattgaat agttacagtt
     1321 attttcttca ataagttttt tacagatttc ctctctaagt atttttaatc ctggaactaa
     1381 atcatattta gtgtaatctt tatttaaaga atcaatacca taagattttg cgttattagg
     1441 tacattaaaa tcgggttctc ctatactaag atttattact ttaactccat tactttccat
     1501 ttctttaact ttagaactaa tacctattgt gtaggatggt gttataaagt ttagtctttt
     1561 tgataacata aaatgcctcc ttttaaatta ttatttatat tatacgacaa atttaaagaa
     1621 tttacataag gaaatttgta gatatataca ttaatatata taatagccta cactttttgc
     1681 ctaaaattag tgtaaaaaaa agagaaaaaa attttaactt tcaaagttgt tagaaacgct
     1741 actgttcaaa atatccacat agtatttgca atgatttgcg caagtttgac tattatgtac
     1801 aaatttggta atcttaatat agcaattatt aagataatcc ctttaactta gttgaagggg
     1861 atttttttag acttaacata ctacatatag tggtatgggg attaataaaa tacaatatat
     1921 agtaacagga ggttttttta attggttgaa ttcgtaaaaa aaagagatgg aagagtaatt
     1981 ccatttaatg aagatagaat aacaagagct atatttttag cagcaacgaa tgttgcagaa
     2041 agagaaggaa tagtacctga ttataaatta tctgagcaat taactcaaga agtaataaag
     2101 tttttaaatc ataaatactc agaatcagtt ccaagtgttg aagatataca agattctgta
     2161 gttaaggttc ttatagaaac tggccatgca aaaactagtg aagaatatat aatatacaga
     2221 actgaaagaa gtagaataag aaattcaaaa acaagattga tgaaggcaat agaggaaatt
     2281 acctttgaag atgcagaaga tgctgatata aaaagagaaa atgcaaatat taatggtaat
     2341 acagcaatgg gaactatgtt acaatatgga agcactgtat ctaaagaatt ttgtaaaaca
     2401 catatattaa aaccagagca ttcttttgct catgataatg gagatataca catacatgat
     2461 atggattttt taaatatggg aacattaact tgttgtcaaa tagatgttaa aaaactattc
     2521 aatggtggat tttcaacagg acatggcttt ttaagagagc cacaagatat aataagttat
     2581 ggtgcattag ctgcaattgc tatacaaagc aatcaaaacg atcaacatgg aggtcaaagt
     2641 ataccatttt tcgattatgg attggctgaa ggtgtatata agacatttaa gaaattctac
     2701 ataggaaatt tagctaaagc attgaagtta tttaaaggaa ttgaaaataa tgatgtgata
     2761 aagaacatag tatataatac agagaaagaa acaaatcaaa aagttggtct taagagagat
     2821 gaattgtatc taaacttaga aaaagagaaa ttaatacaga cttttgatat agatgatgaa
     2881 cttgtaaata agatgcaaaa ctttgcattt gaagaatcgt atagagaaac tgataaaaaa
     2941 acatatcaat ctatggaagc atttatacac aatctaaata caatgcactc tagagcaggt
     3001 gcacaggtac cattttcaag tgtaaacttc ggaacagata cttctgaaga aggaagaatg
     3061 gtaactaaga atcttctatt atctcaagaa agaggtttag gtaatggaga gacaccaatt
     3121 tttccaattt taatatttaa ggtcaaagaa ggcataaatt taaatcctga agaccctaat
     3181 tatgatttat ttaaattatc atgcagagta tcagctaaaa gactattccc taattttagc
     3241 tttttagatg cgccttttaa tgcaaaatat tataaaaaag gtgaaccaga tacagaggct
     3301 acatatatgg gttgtagaac aagagtactt agtaatgtgt gtggttcaga aacagttagt
     3361 ggtagaggaa acatatcttt tactacagta aatttaccaa gattgggtat aaaacatggt
     3421 attataaata atgaaaaggc taatttagat ggtttctttg aagaattaga tgagaagata
     3481 aacttaatta tagaacaatt attggagcgc cttgaggtac aaggaaataa aaagatgaag
     3541 aattttccat tcttaatggg gcaaggtgta tggaaaggtt ctgatgattt aggaccagaa
     3601 gatacactaa aagaagtaat aaaacaagga acattgacaa ttggatttat aggtttagca
     3661 gagtgcctta ttgctcttat aggtaaacat catggagaaa gtaaagaagc tcaagaatta
     3721 ggattaaaaa tagtgtcaca tatgagacat aaaatggatg aagcaacgga caaatataaa
     3781 ttaaatttct ccttaatggg tactccagca gaaggtcttt caggtaggtt tactaaaata
     3841 gataaaaaag tttatggaga aattaaaggt atcacagata aagaatatta tactaattca
     3901 ttccatgtac cagtttatta caatataagt gcatatgata aaattgagat agaagctcca
     3961 taccatgaat tgactaatgc aggtcatata acatatgtag agttagatgg agatccatct
     4021 gataatttag aagcttttga aactgttata aaagctatga aagatttggg tataggatat
     4081 ggaagtataa atcatcctgt agatagagac ccaatatgcg gtttttcagg tgtaataaca
     4141 agtaatatat gccctgtatg tggaagaaat gaagatgaaa gtgacatcaa attcgaaaga
     4201 ataagaagaa taacaggata cttagttggt acagttgata gatttaataa tgctaaaaaa
     4261 gcagaagtaa gagatagagt aaaacataga tagttttatt tgggggaata agttatgaaa
     4321 ataagaatgt catctactat aagttatgac agcatagttg atggaccagg attaagaatg
     4381 gtaatatgga ctcaaggatg tattcataat tgtaaagagt gccataatcc tcagacacat
     4441 gatttatgtg gaggatttta tatggataca gaagaaatta taaataaagt caaatcattg
     4501 aaattacaaa aaggcattac gctatcagga ggagaaccat ttttacaacc agaaccatta
     4561 gaagaaatag caagagaagc taaaataaat gggttagatg tatggtcata tactggattt
     4621 acgtttgaac aactgttaga taaaaaaaat agagcgtatt ttaaaaattt aaatttacta
     4681 aagcaaattg atattttagt ggatggaaaa tttatagctg aaaaaaaaga tataagctta
     4741 aagtttagag ggtcatcaaa tcaaaggata attgatgttc aaaaaagttt gaaatataaa
     4801 aaagtgtttt tggttgagca atatatgaag gatgatttat ctatagcaga ataaaatata
     4861 aataaacagg attaaatata tgcatatatt taatcctgtt tattttcata taatatttca
     4921 agcttgtata atgtaacaat taaaccaaaa gactcattgt atatttgaaa ttaagttttt
     4981 tatagtttat aggtgtttta tgtggattac gaatgatata gtattgatac attatttaat
     5041 agattcttat gccaagaaac aattaattta tagaaattct taattgttac aatataaata
     5101 tctagataag ttataagata ctgataaata atttataaag tttattaatc taatattgtg
     5161 tgtgaatatt aagtggtcaa gtagtctaaa aaatgaaaaa gatttaaaaa agtgttttaa
     5221 ctaaacacat aagtgttaaa ttcatgtata agtataggtt agatacataa gaaataatta
     5281 tgtaatatct atctttgtaa aaaaatatta aattttgtaa tttgtggata aatatgttta
     5341 taatgtggat aatatttaat gtaataagtt aaagcaatat gaatcaactg tttataatgt
     5401 ggataatatt ataaatgtat gaataattgc agtatttaaa aacaaaaaaa gttaaaaaag
     5461 ttgttgactc taaaagaaaa agttggtata gtaatacttg tcctcaagaa aagggcaaac
     5521 aaataaagca taa
//
LOCUS       DAGSRM010000146         5422 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.152, whole genome shotgun sequence.
ACCESSION   DAGSRM010000146 DAGSRM010000000
VERSION     DAGSRM010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5422
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.152"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(591..1997)
                     /locus_tag="MJE33_003469"
     CDS             complement(591..1997)
                     /locus_tag="MJE33_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY9649377.1"
                     /translation="MDSVKYILRRFIGSSILISLLFIVLNIAGFLLFSYMLFVSKYSD
                     NLDTSDSREIKSNLIMIEDNLILRNGKFILDDSSKEVLHKNKVWAMLIDNNGDRIWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEHPNGILVTGYPKDKYTKFNLTYTVKE
                     IEQFPMLIIGMFLVNAIVTFLIALFIGLKMVKSIKPIITGIKLMSKGEPVLLQEKGIL
                     SDISKSINTVSKELQMKDEKLRKKEEARSNWIAGISHDIRTPLSMIIGYAGELEESSS
                     LSNREQEQVSIICNQGIKIRELVNDLNLVSKLEYNMQTLNCDKIRVSAFLRELISEFI
                     NNNLDNDFYVELDILNEDIIIDADKKLLKRAISNLIQNSITHNQQGCNIRVTSKSDLD
                     FCYIIVEDDGKGMSQERINSLLKGQNGLSEAYAKGQNHGLGILIVSGIINAHRGKLDI
                     TSIDSQGLKTILKLPLIK"
     gene            complement(2042..2749)
                     /locus_tag="MJE33_003470"
     CDS             complement(2042..2749)
                     /locus_tag="MJE33_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY9649378.1"
                     /translation="MESIKDKKILVVDDHKELLKMIDEILRKEGFSRVFLASSYEEAV
                     RVFRNVKPDCAILDVVLPDGDGFSIMRKIRETSKIPVIFLSARGEDEDRLIGLGLGAD
                     DYIVKPFLPKELTLRLVGILNRVYVPIEEEELPVFKLGDSVVVNLNSACVEKDGQEIS
                     LTAKEHSLLLKLYENKGRIVTNDSLCQAIWGDDMYGYENTLMVHVRRVREKIEKTPST
                     PKHLITVRGLGYKLMIN"
     gene            2920..3834
                     /locus_tag="MJE33_003471"
     CDS             2920..3834
                     /locus_tag="MJE33_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9649379.1"
                     /translation="MEMIIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGAGKS
                     TTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENMTIM
                     QRLLDLPEKNVNEALKIVRLENQKNKKVSQYSLGMKQRLGIAMAIMKFPKLLILDEPT
                     NGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQDSIK
                     ELHNRGQSQIAIKTKNVVSAQKLLNTKGYMSLIQDGYLTIENLKDSDVAKINGMLVHS
                     NIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            3834..4595
                     /locus_tag="MJE33_003472"
     CDS             3834..4595
                     /locus_tag="MJE33_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9649380.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFVAVEIMWCIINSNRAITRNPD
                     MLGGFEYSYMLMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYL
                     SKFLCSAILVSIPVLMQVLSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHL
                     WIATVIPNQMIAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDMNFSITILVFLIGMLIYYIGRNHLLKKEV"
     gene            4607..5344
                     /locus_tag="MJE33_003473"
     CDS             4607..5344
                     /locus_tag="MJE33_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9649381.1"
                     /translation="MLRKSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSK
                     LTVVSILTAITQVFFVIFYIVAGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVAKPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     TICVAFITTIVFSEIANRNFRKNDVIS"
BASE COUNT         1881 a          836 c          702 g         2003 t
ORIGIN      
        1 cattaattat attataaatt ttccatatat ttcaacataa atacatatat atatatgtta
       61 tttctaaaaa tactacacaa aaactctact ttagtagaac ttaatggcgc aaaatacttc
      121 aatttaaaac atttatcgta ctttaaataa ataactataa taatatactt acaatagtta
      181 catagaagct attataacta cacagaacag cgtataatta aaactttttt aggatgtcat
      241 tatatttttt cttaaaatta aaaaacacta taccataatt ctattttagt agaatttttt
      301 tgtcaataat aaaattaaat attaactaaa aaataaaaaa aatggaatta tctaaagaat
      361 ttaataacct tacaataatt ccaaaatatt atttctatga aaattttgat accatgagta
      421 actcagccat aagcgcataa agcacaagtt actctttact ttaataaaac ttgacaagaa
      481 aagaatttat ccttattcta ctctaataga gttaatttgt caataaagaa taaaattatt
      541 ttcaaaatat tttcattctt cattcttatc tgttattatt ttataaaata tcacttaatt
      601 agaggtaatt ttagtattgt ttttagtccc tgactatcaa tgctcgtaat atctaattta
      661 cctctatgtg cattaataat gccagatact atcaaaatac ctagaccatg attttgacct
      721 ttggcatatg cctcacttag tccattttgg ccctttaata aactattaat tctttcttga
      781 ctcatacctt ttccatcatc ttcaacaatt atatagcaaa aatctaaatc agacttagag
      841 gtaaccctta tattacatcc ttgttggtta tgagttatac tattttgtat taaattacta
      901 atagcccttt ttaataattt tttatctgca tctataataa tatcttcatt tagtatatca
      961 agttcaacgt aaaagtcatt atccaaatta ttatttataa actcagaaat aagctctctc
     1021 aaaaacgcag aaactctaat cttatcacaa ttcaatgttt gcatattata ttcaagttta
     1081 gacactaaat tgaggtcatt tactaattct ctgattttaa tcccttgatt gcatataata
     1141 gacacttgct cttgttctct atttgaaagg ctactgcttt cttcaagttc tccagcatat
     1201 ccaattatca ttgaaagtgg agttcttata tcatgagata ttcctgcaat ccagttagac
     1261 ctagcttcct ctttcttcct aagtttttca tctttcattt gcaactcttt agatacagta
     1321 ttaatagatt ttgaaatatc acttagtatt cctttttctt gtaaaagtac tggttctcct
     1381 tttgacataa gctttatgcc agtaataatt ggttttatag atttcaccat ttttaaacct
     1441 atgaatagag ctattaaaaa agtaactatt gcattaacca gaaacatacc tattattaac
     1501 ataggaaatt gttctatttc cttaaccgta taagtaaggt taaacttagt gtacttgtct
     1561 tttggataac ctgttactaa tattccattt ggatgttccc atacatacac aggatagtcc
     1621 tttaaaaaga accttgaaaa ctttgctaca tctgtcaaag tataatgact tggaatttct
     1681 tttggtaaat caaatcccca tattctatct ccattgttat caattaacat tgcccaaact
     1741 ttatttttat gtagtacttc cttactagaa tcatctaaaa taaactttcc atttcttaaa
     1801 atcaaattat cttcaatcat aatcaagttt gattttattt ctctgctatc acttgtatct
     1861 aaattatcag agtatttaga tacaaatagc atatatgaaa acagaagaaa acctgctata
     1921 tttaatacaa taaaaagtaa tgaaatcaag atacttgaac ctataaatcg cctcaatatg
     1981 tactttacac tatccaaaat taaatcactc ctcttgattt cttttattct aatatctaaa
     2041 attaattaat catcaactta tatccaagac ctcttactgt aataagatgt ttaggtgtag
     2101 atggagtttt ttctatcttc tctctaactc ttctaacatg aaccataagg gtattttcat
     2161 atccatacat atcatctccc caaatggctt gacatagtga atcattagta acaattctcc
     2221 ctttattttc atacaacttt aaaagcaacg aatgctcttt agctgttaaa gaaatctctt
     2281 gcccatcttt ctcaacacat gcactattta aattcacaac aacagaatct cccagtttaa
     2341 atactggcaa ctcctcttct tctataggca catatacacg atttaatata cccacaagcc
     2401 ttaaagtaag ttcctttggt aaaaatggct ttactatata atcgtcagcc cctaagccca
     2461 aaccaatcaa tctatcttca tcttcacctc ttgcagagag aaaaatcact ggtattttag
     2521 atgtctctct aatttttctc ataatagaaa aaccatctcc atctggcaat acaacatcta
     2581 aaattgcaca atctggcttt acatttctaa ataccctaac agcttcctca taacttgatg
     2641 ctaaaaatac tctactaaac ccctcttttc tcaggatttc atctatcatt tttaataatt
     2701 ccttgtggtc atcaaccaca agtatttttt tatcttttat tgactccata atcatcaccc
     2761 tcctttagta taccatatac ccattttcac aactttgcct aaatagactt ttaaggttat
     2821 tgtaaggtaa atttcagcta attgaaatgt aaccaagtta ttatactgat ataagttaaa
     2881 agaatcaata agcacagatt aaaaaagggg atgaaacata tggaaatgat aattagaaca
     2941 caaagacttt caaaggaata caataaaata tttagagtaa aggatataga cctaagagtt
     3001 ccacaaggag ctgtatatgg ttttttgggt cctaatggag ctggaaaatc taccactctt
     3061 aaaatgcttc ttggacttgc taaacccaca caaggaaata ttaatatttt aggtaaagag
     3121 ttaaatgaaa aaaatagaat atcaatttta aaagatatag gttctttgat agaatctcca
     3181 tcatactatg gtcatcttac agggcttgaa aacatgacca taatgcaaag actcttagat
     3241 ttacctgaaa aaaatgtaaa tgaagcatta aaaattgtaa ggttagaaaa tcaaaaaaat
     3301 aaaaaagtta gtcaatattc tttaggtatg aagcaacgtc ttggcattgc tatggcaata
     3361 atgaagtttc ccaaattatt aatacttgat gaacctacaa atggactcga cccagcaggg
     3421 attgaggaaa ttagagaatt aataaaatct ctaccagaaa aatacgggat gactgttcta
     3481 atctctagcc atcttttaag tgaaattgac caaattgcca catcaatagg aataataaat
     3541 catggcgaac tggtttttca agatagcata aaagaacttc ataatagagg tcaaagtcag
     3601 attgctatca aaactaagaa tgttgtatct gcacaaaaac tattaaatac taaaggatat
     3661 atgtcactta tacaagatgg gtacttgact attgaaaatt taaaagactc agatgtggct
     3721 aaaataaatg gtatgcttgt ccattcaaat atagatgtat ttagaataga agaacataaa
     3781 aaaagcttgg aagatatatt cttagaactt actggtaagg cggtgacttt ataatgaaat
     3841 accttggtct agaattttat aaacttaaaa gaaagaagat tattctgatg acctttttat
     3901 ttgtagctgt tgaaataatg tggtgtatca taaattcaaa tagagctatt acacgtaatc
     3961 cagatatgtt aggtggattt gaatattcct atatgcttat gagttttgca agtttaaatg
     4021 gtctattctt tcctatctta atatcaataa ttacatcacg tataagcgat atagaacata
     4081 aaggagatac atggaagctt ttaaaatctt ctgtgacatc tttaaatagt atttatttat
     4141 ctaaattttt atgttctgct attttggtat ctatacctgt attgatgcaa gtgctatcta
     4201 ttgtgttgtt tggacatttt agaggggtta ttgagtcaat ttctgtatct ttacttttaa
     4261 aatacacatt aggaacaatt cttgtaagta ttgctatcat tgcactacac ctatggattg
     4321 caactgtaat tccaaatcaa atgattgcaa ttacatttgg catgattggt agttttatag
     4381 gtttgacatc aggtttgttt tatcaaggta ttcgtagatt gtttatatgg tcttattacc
     4441 tagagttatc acctttaagt tatgcttatg ataatgtttt aggttcatct gtctatagaa
     4501 ttgatatgaa tttttctatt acgattttag ttttcttaat tggaatgcta atttattata
     4561 ttggaagaaa tcatttatta aaaaaagaag tataggaggg atttaaatgt taaggaagtc
     4621 agttttggga gaaattctaa agttaaaaaa ttcttttata tggtatgtat tattagcttt
     4681 gccacttata agtgtgttga ttggttctgg taatttttat ctaaatcaag gcatactcaa
     4741 aaaagaatgg tacagcttat ggactcaagt tagtcttttt tatggggaat ttttctttcc
     4801 tatcttaatc gcaatctttt gtgcatatat ttgtaggctt gaacatatga atcataattg
     4861 gaataatgta atgacactgc cagttaattt aaaaaatatc tttttatcta aacttacagt
     4921 agtttcaata cttacagcta ttacacaagt tttctttgtt atcttttata tagttgcagg
     4981 aaatatgttt ggtttttctg gtagtgtacc taaagaattg actggttgga ttgctaaagg
     5041 ttggattatt tctatttcaa tagcttctat acaattatac atgtctataa agattagaag
     5101 ttttgcaact cctattggta ttagtctttg cctttctcta tttggtatgg gattaatagt
     5161 agcaaaacct gctgttggta tatttttccc ttattctatg ttaggtagtg ctatgggggt
     5221 tataaagcaa tctgctttaa atgttaatga cactatagtt acaatatgtg ttgcatttat
     5281 tactactata gtattttctg aaattgccaa tagaaatttt agaaagaatg atgttatttc
     5341 ttaatggtaa atgctagata ttttggggtt tgaaatttat attcttttta gttgaaatct
     5401 atatccattt tatttaaaaa ta
//
LOCUS       DAGSRM010000147         5416 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.153, whole genome shotgun sequence.
ACCESSION   DAGSRM010000147 DAGSRM010000000
VERSION     DAGSRM010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5416
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.153"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(435..635)
                     /locus_tag="MJE33_003474"
     CDS             complement(435..635)
                     /locus_tag="MJE33_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBY9649382.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     QKVSFDIVKGARGPQAENITIL"
     gene            complement(860..1036)
                     /locus_tag="MJE33_003475"
     CDS             complement(860..1036)
                     /locus_tag="MJE33_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649383.1"
                     /translation="MFRDKMDKCTHMLTAYIGSSYDYCDFIDTHLDDFILEYGEKVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(1090..1179)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1359..1559)
                     /locus_tag="MJE33_003476"
     CDS             complement(1359..1559)
                     /locus_tag="MJE33_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY9649384.1"
                     /translation="MPKFTCNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
     gene            complement(1561..1911)
                     /locus_tag="MJE33_003477"
     CDS             complement(1561..1911)
                     /locus_tag="MJE33_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HBY9649385.1"
                     /translation="MIQKKFFMGFCGFLGFLSLRYFSSGNVADLTYIGFFAFFSNFII
                     AKINGDKADERYVQDEKAAMAFTGQLAIIELFILWCITIVSRNIELMCVLLSITYAIT
                     LNVYAIKLYILEEK"
     gene            2073..2261
                     /locus_tag="MJE33_003478"
     CDS             2073..2261
                     /locus_tag="MJE33_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBY9649386.1"
                     /translation="MTKYDFNFKLKVAKFYLNREGRDILIVKQYGVYDHSQVERWVNI
                     LGESELKIKKFSILYGSS"
     gene            complement(2738..2908)
                     /locus_tag="MJE33_003479"
     CDS             complement(2738..2908)
                     /locus_tag="MJE33_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649387.1"
                     /translation="MHVYQPKTSEYLLNTSAKSYLSSIVFLFDYITKNESRYAFFIID
                     FPFLSLDIFYKF"
     gene            complement(3297..3785)
                     /locus_tag="MJE33_003480"
     CDS             complement(3297..3785)
                     /locus_tag="MJE33_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBY9649388.1"
                     /translation="MKNKKGFTLVELLVVIAIIGILAIVALPALFKNIEKAKIAKLEA
                     DISAIKSASLSYYADESKYTEGNIIWWTKKDGKITVNSGIGDEDPLAHKIENLGMPYN
                     GSYTLVSSNGSEEYLELNIIIDGEISKSGLDKLEEDYGSSIKIPNDKNMIITFLSNKS
                     DN"
     gene            4591..4965
                     /locus_tag="MJE33_003481"
     CDS             4591..4965
                     /locus_tag="MJE33_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBY9649389.1"
                     /translation="MKEEILIEKLLRAELIVMEYLWKKDTLMPKKEIVKEIKQIYGWH
                     KSTIKILLKRLVAKRYLARYIINSQSHYKIIDSKEYYTFKKKVLKSSKSIKIMRSLTT
                     THKNVSKEKLDLLDEYYRNLKE"
     gene            5070..5416
                     /locus_tag="MJE33_003482"
                     /pseudo
     CDS             5070..5416
                     /locus_tag="MJE33_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT         2180 a          656 c          617 g         1963 t
ORIGIN      
        1 aattaaaaac ttaaatcaaa ttaacttatt gtagattttt tactttttaa ctaattttat
       61 atttattata tttcagtgaa aaaatagaac attgaaacat atagatatgt tttaattgtt
      121 ttttattaac aaattactaa aagtgttata tcagaattga atacaaaaga aagagtatgt
      181 atttatgaga tagttgaaaa ttttgatgtg ccacaactaa aattattata ttactatata
      241 ttctaattaa aagagtactt gtaggtttaa gtacatactt tataaatata tttttgtcag
      301 atttatttta taaaaagtat ttagactaag tattattttt aagtattttc ttgttgaagt
      361 tattttttcc ttttttacaa aatgcgtaaa taaaaaaaag actcttaaaa gagtctttcc
      421 gaaaaatcta tatattataa tatagttata ttttctgctt gaggacctct agcgccttta
      481 actatatcaa agcttacttt ttgaccttct tctaatgatt taaatcctga agtttgtata
      541 gctgagaaat gagcaaatac gtcatctcca ctttccattg atataaatcc aaatcctttt
      601 tcattattaa accattttac tattccatta ttcattaaaa atactcctat taacctatat
      661 caagatatac ttgatttact tgtgtattac cacaaatact attgtatcat agattaacat
      721 aaatgtctat ttaattataa tttttagtta acatttttat ttattcttaa tatgaaaata
      781 aatttaaaca ttttatatta ccaatatatt aatgtaaatg tagataaaac taaaaagggt
      841 tttctagaaa aaaatctgtc taattatact tacttaccaa taccatcact tgatgcaagc
      901 aagattccac aactttttct ccgtactcta atataaaatc atctaaatgt gtatctataa
      961 aatcacaata atcatatgaa ctaccaatat aagcagttaa catatgtgta catttatcca
     1021 ttttatctct aaacataatg tatttatccc cctaaaaaac tttcatgaaa aacaaaaaga
     1081 aaatatcttt ttggcaactg gctgacataa cttattttat aagcgttagt ccctatagct
     1141 ttgcgtcact aaatttctct agttttgccg atttagtttt attctacaga ttaataatac
     1201 aatagaatta gttattattc aacatatcta gctgaaaaat taataaattt atatatgaac
     1261 gttaaattat ctgaaaaaat aaaaagcccc tctgtcagga gtgacttaaa aattgaattt
     1321 aagtttgtat ttaaaacaca tttttaataa tgcaaaaact aatcaaaaat aaacaattca
     1381 tcaataggtg tatctactat ttttgatata tctattgcta atttcaaaga aggattatac
     1441 tttcctgctt ctaaacgcat aatggtttct cttctaactc ctactttttc tgctaattgt
     1501 tcttgagtta attcttttaa ttgtctatat ttttttaggt tacaagtgaa tttaggcata
     1561 ttatttttcc tctaaaatat aaagttttat agcataaaca tttaaagtga ttgcatatgt
     1621 aattgatagt aaaacacaca ttaattctat attcctagac actatagtaa tacaccataa
     1681 aatgaataat tctataatag caagctgtcc tgtaaatgcc atagctgctt tttcatcttg
     1741 tacatacctt tcatctgctt tatcaccatt tatttttgct ataataaagt ttgagaaaaa
     1801 agcaaaaaat ccaatatatg taaggtcagc aacattgcca gatgaaaaat atctaagact
     1861 taaaaatcca aggaaaccac aaaaacccat aaaaaatttt ttctgaatca ttttttatcc
     1921 tccttagtaa ttttaaaaga gatatattta tatctttatg atacaaatat atctctttta
     1981 tattcttttg tcaatagaaa atcagtatta tacaatattt gtactgcacc ataattgtta
     2041 gagcaaaaat ctactgcttg agatgtttat gtatgactaa atatgatttt aatttcaaat
     2101 taaaagtagc taaattttat ttgaatagag aaggaagaga tattttaata gtaaaacaat
     2161 atggagttta tgaccattct caagttgaaa gatgggttaa tatattagga gagagtgaac
     2221 taaaaataaa aaaattttct atactatatg gttcaagtta gatgttataa actatatgat
     2281 gataactaaa tatagtgtac aataaatcgc taagtatttt ggaataaaca atagcacagt
     2341 tatagcaaca tgaaaataaa aatttttaca tagtggtgtt gatgcctttt ccaaaatgaa
     2401 aggaagacca taatgaataa aaacaaaaac actaaagaaa aaataaatta acaagagaac
     2461 aagaattaga atatgaaaat aaatttttaa aagatgagct tgagttttta aaaaagatgt
     2521 gtgcctcagg gaagccacat taaaaaacag tggtatcatt attaatcaaa agaaagttag
     2581 atgtattatc aaaaaattag aacttaaatg catagcattt tttcataaat cacataaata
     2641 taactcctat aaatgtataa ttggaaaagt aaaaaataat aaaacaaata gaagatttaa
     2701 aacatcaata gcatatcaga aaataacaaa tgatatgcta aaatttataa aatatatcta
     2761 atgataaaaa tggaaaatca attataaaaa aggcatatct cgattcattt tttgttatat
     2821 agtcaaataa gaatactata gatgaaagat acgattttgc agatgtatta agtaagtatt
     2881 ctgatgtttt tggttgatac acatgcaata aagaagttgt gtttgcagat aaattagcaa
     2941 aactatagta taagttgcaa caataaagaa aatgaaaatt atcatactaa agcataaggc
     3001 atgttacaat tttattagat aggacattaa catacactaa agtacttttt actataaaga
     3061 gtacttattt ttttgaaatt cattaatgta taaactatca agaatattac tcaacataat
     3121 ttaaaaacaa cttgaatagg tatttttaaa ttatgtgata taataaaaaa tagaagtgta
     3181 tgccaataca cttctgtagt tataactaag cattctattc ttgaggagtg cttttttcat
     3241 ttttataacc aactcttttt actttaacaa taattatcag acctatatat ttatttttaa
     3301 ttgtctgatt tattagataa aaaagttatt atcatatttt tatcatttgg tatttttatt
     3361 gaactaccat aatcttcttc taatttatct agaccacttt tacttatttc tccatctata
     3421 attatgttta attctaagta ttcttcacta ccattagatg acactaaagt gtacgaacca
     3481 ttataaggca tgcctaaatt ttctatttta tgtgccaaag ggtcttcatc accaatacca
     3541 gagtttactg ttatttttcc atctttttta gtccaccata ttatgtttcc ttcagtatat
     3601 tttgattcat ctgcatagta gctaagagac gcacttttta ttgcacttat atcagcttca
     3661 agtttagcta tctttgcttt ttctatattt ttaaataatg ctggaagtgc tactattgcc
     3721 aatattccta ttatagcaat tactactaat aattccacta gagtaaatcc ttttttgttt
     3781 ttcatttatt tcccctccat ataaaattaa taatatataa atttttattt tgttgatttc
     3841 tactttctat attttaccat atatagacaa aaaaatcact ttctatgaca aatatactat
     3901 tattttacaa tttatggaaa tgatttaaaa tgacatttct tgttagaaaa gatataagca
     3961 atggattaaa ataaagaaaa ctacagtcat atcaacgcca ataatattat ttaaaagcag
     4021 atgaaaataa aaagagggcc cagaaaataa tgtctattta attatactta cttaccaata
     4081 ccataacttg atataaacaa gattatacta cattttttta tgttctagta taaaatcatc
     4141 taaccgtgta tctataaaat caaaataatt atatgaacta tcagtataat aagtcaaaat
     4201 atgtatacat gaatacatat tttttaaaag atatatatta ttacatacct aaaaaacttt
     4261 cataaaaata aaaataaaat atgattttag taacttgtta acataactta taatttattt
     4321 tataagcatt agcctatata gctttgtgct actaaatttc tttgattcta acgatttagt
     4381 gctattctac aactaaaata atacaataga gctatttatt gtttggtagc attgtttgca
     4441 aattaaacaa atttatatat atgaatatta ttttacgttc aaaaaaacag actaaatatg
     4501 tagaatattc ggtaaaactt acctctagtt tttgtcttaa aaagctccta ttattaactt
     4561 aagttagaag aattaaggaa gtgacataaa ttgaaagaag aaatattaat agaaaaatta
     4621 ttaagagcag aattaatagt aatggagtat ttatggaaaa aagacactct aatgcctaaa
     4681 aaagaaattg taaaagaaat aaaacagatt tatggatggc ataaaagtac aataaaaata
     4741 ctactaaaaa ggctagttgc taaaaggtat ttggctagat atattataaa ttctcaatct
     4801 cattataaaa taatagatag taaagaatat tatactttta agaaaaaggt actaaaatct
     4861 agtaaaagca taaaaataat gcgttcactt accactacac ataaaaatgt aagtaaagag
     4921 aagttagacc tattagatga gtattataga aacttaaaag aatgatatag taaaaatcga
     4981 atgtaattat ttaccttaat tcttttgttt gtaaaacttt attgatacat aatttaacat
     5041 atacataaat tggtaaagga atgtatacta tgaaaattag taaattacca gaagcggaat
     5101 taaaagttat gagatatatt tgggaatcag aaaaggtatt aatatcaaga gaaatcacca
     5161 agtctatgga acaaaaatat gaatggaaag atacaactat atttacagta ttaaaaagat
     5221 tactaaagaa gagagttttt agaaacggag aaaatagata agtgtacaca ctataatgta
     5281 ttagtaaaaa aaaataatat cagagatttt aaacaaaaga atttttaaag aatacacata
     5341 aaaattcaat attaagttta atgtcagcac tatgtagtaa ggatgatgaa gtagatggtg
     5401 atattttaaa tttatt
//
LOCUS       DAGSRM010000148         5250 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.154, whole genome shotgun sequence.
ACCESSION   DAGSRM010000148 DAGSRM010000000
VERSION     DAGSRM010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5250
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.154"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            241..1767
                     /locus_tag="MJE33_003483"
     CDS             241..1767
                     /locus_tag="MJE33_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBY9649390.1"
                     /translation="MNESYWFLNSSPKEYNKLGENIKTDCLIVGGGITGLTTAYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VEQIIEENNIKCDFKRETSFVFTEKEDTIKDIKDEYRTCKKIGINCEYHETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKAAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMVAVLENKLADGMFISIDDPSITFRQY
                     NDGSENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKEVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDNIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            1795..2352
                     /locus_tag="MJE33_003484"
     CDS             1795..2352
                     /locus_tag="MJE33_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649391.1"
                     /translation="MNKLLKPITILLYIVYILSAVFFVANKEYGSLGLVAISLVGTFI
                     LSFINKQTERLLSKELYIMLALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCIVA
                     FSIIKYFYTQREISKMSKVFLVIFLFMFSMGVASLWEIGEFLMDTFVGTTTQAGGLED
                     TMIDMVDALIGTIITIPFIVKKSNI"
     gene            2874..3911
                     /locus_tag="MJE33_003485"
     CDS             2874..3911
                     /locus_tag="MJE33_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649392.1"
                     /translation="MLKKWFGIVKKRQKSESVKEEGEVILKNEKILSEEKLIDEEGVV
                     VNIDNQILTKVVNDDNEIEEKIIEEDLLISENTIKLDDKEAIINDKNIELCKKEVQVE
                     SEKIGLNKFEGLDQNENYNLEKNVIEEKKVSECLTEEDLEYIKEIKIKRGKSIKAINL
                     YTKEEWVFDTHIQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKSEW
                     SYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYRFECIDEEYDEY
                     FKKYKSIIKRGGKKKVELVNKKGDILEIFKSLEECAIYLQKEKNEVIQMLKYGDTKVG
                     RNFIRYSLRSI"
     gene            4022..5164
                     /locus_tag="MJE33_003486"
     CDS             4022..5164
                     /locus_tag="MJE33_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBY9649393.1"
                     /translation="MSEEKNPKENTLDESKNSDNVNSKKDDVSDEKNTDNKVKKREIK
                     LNNKTLKAIVAIVAVGAICLGGGYVFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKSNYLQVRASHILIKTVDDKGKQVSSSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEVKSDLVSSLTSEKQNLLIQNLKEKYNVKITDKYQMKAKE"
BASE COUNT         2214 a          438 c          935 g         1663 t
ORIGIN      
        1 ttctaacgaa attggatatt tcttctgtaa atacatattc aaaaaaagaa ttataagagt
       61 ttagtcagca gtcgtgtaag taataaggac tttataaata tatagaaata taatgatatt
      121 aatataacca agcgattttt aatttagctt tttaataagc ttatttttta tactatttat
      181 aataaataaa ctccatgatg ggaaaactat tactatttaa tatgtaaagg agtattttaa
      241 atgaatgaat catattggtt tttaaactca agtccaaaag aatataacaa attaggtgaa
      301 aatataaaaa cagattgtct tatagtaggt ggtggaataa cgggattgac aactgcatat
      361 ttattagcaa aagaaggtaa aaaagtagtg cttgttgagg ctgataaaat aggatatgga
      421 acttctggaa ggaatactgg taaggtaact tgtcagcatg atatatttta ctctaagata
      481 gagaaaaaat atggattaga taaagcaaaa tcatattata atgcaaataa tgaagcttta
      541 aatttagttg aacaaataat agaagaaaac aatataaaat gtgattttaa aagggaaact
      601 tcttttgtat ttacagaaaa agaggatact ataaaggata ttaaagatga atatcgtaca
      661 tgtaaaaaaa taggaataaa ttgtgagtat catgaaacta tagaaaatat tccattagat
      721 ataaaggggg ctattagttt tactaatcaa ggtcaattta acccaaagaa gtatattgat
      781 ggattggcaa aagcagcagt aaatttaggg ctaaaaatat atgaaaatac accagtagta
      841 gaccttgaaa aaggaaaaat atgtagggtt aaaacaagag aggataatat aatagaagct
      901 gagaatgtta taatttcatc tcatagtcca tggtatgatg gattgaattt ttattttgca
      961 aaagaatatg cagagcgtgc ttatttaatg gttgcagttt tggaaaataa attagcagat
     1021 ggtatgttta taagtataga tgacccatca ataactttta gacaatataa tgatggaagt
     1081 gaaaatttac tgatttttgg aggaggagac cataaagttg gtcagggagg aactgagaaa
     1141 gaaatatttg atgatttaga gcattatggt aaagaagtct ttaaggtaaa agattttaag
     1201 gggaaatggt ctgctcaaga taatatgagt tttgacaatg ttccttatat tggttatatc
     1261 aacaaaagag aagacaatat ttatgtagct acagggttct ctaaatgggg aattactaat
     1321 ggaactgctg ctggaataat aataaaagat ttaataataa ataataattc tgattataaa
     1381 gatactttta atccatccag actaggtagc tacttttcaa aagattttat aaaggaaaat
     1441 gcaaacgtag caataaatta tgtaagtgga aaattaaaaa taggtagtgg agatatgcct
     1501 aaaaataatg gagaaggaaa gattgttaat attgatggaa aaagatatgg tgtatataag
     1561 gatgataatg gagattttta tattgtagac actacatgta ctcatttagg atgtgaactt
     1621 aattttaaca gtgaagaaaa aacatgggac tgtccatgtc atggttctag atttgactat
     1681 aagggaaata tacttgaagg accagctctt aagccattaa aactttatgg acatggtgat
     1741 aatgatgtta atcctaagtt attataaaat tatttttagg aaggtaaagt gtatatgaat
     1801 aaacttttaa agccaataac tatactattg tatatagttt atattttatc tgccgtattt
     1861 tttgtagcaa acaaagaata tggaagtcta ggtctagtag caattagttt agttggtact
     1921 ttcattttga gttttataaa taagcaaact gaaagattat tgagtaaaga attatatata
     1981 atgcttgctt tatttataat gttttcatca ttgttaggta cttgttttaa tttttatgat
     2041 ataaattatt atgatgattt tttgcattta tggtcaggta taatatcttg tattgtggct
     2101 ttttctataa ttaaatactt ttatacacaa agagagataa gtaaaatgag taaagtgttt
     2161 ttagttatat ttttatttat gtttagtatg ggagtagcga gtttatggga aataggtgaa
     2221 tttttaatgg atacttttgt tgggactact acacaggccg gagggttaga agatactatg
     2281 attgatatgg tagatgcttt aataggcaca attataacta ttccttttat agtaaaaaaa
     2341 tctaatatat agttttaaat tttttatatt aattataatg gatttaatta atcatgttga
     2401 gtttcaaggt ctatatgatt aattaaatcc atttttgtta tttataatta ttattgaata
     2461 tatttttaca tataagatat aaggtgatag atagaaattg ttggattatg taaataatta
     2521 atatataata ccattatggg caataaatac aaatcaaatg ataatatagt ctatttatgt
     2581 aaatgttata ttgtatccta agtataatag taagtttttt gatagtggtg tagaaagtaa
     2641 aaatctacta gcctaaattg atgtatggat tagtttagga acagtaattg catatcttaa
     2701 gtttgatgtc atgttgggct actagaaata ggtcatcttt ttcacttgaa tatcttttct
     2761 ttaaacatgg ggagtatcat atcaaaaaca tatgaaaatt aagtgattta tgagaacagt
     2821 aatattttat atgatagtat agaatagtaa taaagtatta ggaggaacat tgagtgttaa
     2881 aaaagtggtt tggtattgtg aaaaaaagac aaaaaagtga gtcagtaaaa gaagaaggtg
     2941 aagtaatatt aaaaaacgaa aaaatattat ctgaagaaaa gttgatagat gaagaaggag
     3001 ttgtagttaa tattgataat caaatattaa ctaaagtagt aaatgatgac aatgaaatag
     3061 aagaaaaaat aatagaagaa gatttgttaa ttagtgaaaa taccataaaa ttagatgata
     3121 aagaagcaat tattaatgat aaaaacatag aattatgtaa aaaagaagtt caagttgaaa
     3181 gtgaaaaaat aggtttaaat aagtttgaag gactggacca aaatgaaaat tataatctag
     3241 aaaaaaatgt tattgaagaa aaaaaagtaa gtgaatgttt gacagaagaa gatttagagt
     3301 acataaaaga aattaaaata aaaagaggaa aaagtataaa agctataaac ttgtatacta
     3361 aagaagagtg ggtttttgac actcatatac agtgtagtaa aaaactcaaa gttccattag
     3421 ggtacataag agaaaattta aaatatggat atatggatta ctttggagat gcaataaatt
     3481 atttaagtga agtattaaat atagatgaat actgcaaaag tgaatggagc tatctcgata
     3541 atagcaaatc tccatctgag atatttaata ttctaaacaa taaaatattt agtataaggc
     3601 tttctaatga aaaaagaaat gaaattttga caaatgataa aattgaagca ttaaaaatga
     3661 attatagatt tgaatgtatt gatgaagaat atgatgaata ttttaaaaaa tataagtcta
     3721 taatcaaaag aggtggaaag aaaaaagttg aattagtaaa taaaaaaggt gacattttag
     3781 aaatatttaa gtctttagaa gaatgtgcca tttatttgca aaaggaaaag aatgaagtta
     3841 tacaaatgtt aaaatatgga gatacaaaag taggaagaaa ctttataagg tatagtttga
     3901 gaagtattta atttcatttt aaattcataa gagtttcatt tctgattcac acatttaata
     3961 ttattttaat gtaaaataat aaaggtgaag tattgcacaa ttaaaattaa ggagggtatt
     4021 aatgtctgaa gaaaaaaatc caaaagaaaa tacattagat gaatctaaaa attcagataa
     4081 tgtaaattct aaaaaagatg atgtgtcaga tgaaaaaaat actgataaca aagttaaaaa
     4141 aagggaaata aagctaaaca acaaaacact taaagctatt gtagcaattg ttgcagtagg
     4201 agctatatgt ttaggtggag gctatgtttt tggcaaagaa gctggaagaa aattgcctgc
     4261 tacttcaaaa cactataata gcagtgaggt agtagctact attggggata aaaaaataac
     4321 aggtgaaatg ttaagaaaga gaatggagcc attattttat ttaaatgcaa aaactactat
     4381 gactgatgac gaaatacgtt catatgaatc aaatatgatt gaaactatga ttacatctga
     4441 tatattatcc tctgaagcag caaaagagaa aataagtata actaatgacc aactagatgc
     4501 tcagtataat aatttaatga gtagtataga atcagcaatg ggaatgacta aagaagagtt
     4561 tttaaaacag tttgatttga ctgaaaaata tataaaagat gatatgaaaa aagagctagt
     4621 agcatcaaaa tatttaaatg aaaaatcaaa agttactgat aaagaagcag agaactatta
     4681 caataaaaac aagagcaatt atttacaagt tagagcttca catattttaa taaaaactgt
     4741 tgatgataaa ggtaagcaag ttagtagtag caaaaaagct gaacttaaga aagaagctga
     4801 agaaatactt aaaaaagccc aagctgggga agattttgct actctagcta aaaaatactc
     4861 tgaagatagt agtgcagaat caggtggaga tttaggtttc tttggtaaag gtcagatggt
     4921 ggaatctttt gaaaaagcag cctttgcatt gaagaaggga gaggtatcta acaaacttgt
     4981 agaaagtgat tatggatatc atataattaa gaaaactgat gaaaaatatc agccatttga
     5041 agaggtaaaa tcagatttag taagcagttt aacatcagaa aaacaaaacc tacttataca
     5101 aaatctaaaa gaaaaatata atgttaaaat tacagataaa tatcaaatga aagctaaaga
     5161 atagataaag tcaatttatt aaaaaaaaga aaaattaact gtatggataa aagctatttt
     5221 tactctcaat atagagagta taaaatagct
//
LOCUS       DAGSRM010000149         5167 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.155, whole genome shotgun sequence.
ACCESSION   DAGSRM010000149 DAGSRM010000000
VERSION     DAGSRM010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.155"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            21..650
                     /locus_tag="MJE33_003487"
     CDS             21..650
                     /locus_tag="MJE33_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBY9649394.1"
                     /translation="MKEDKKNILIGADTSLRSLEIDKTIKNYCSGILSSAVSNIVGVQ
                     FRQGARIGASVFSKYGFSDLIPALLVVGAKVRLYNKGILELSEFLDSELSRDILVEVI
                     LPKKDAIAVFDSIRKCTGDFSVLNSAMLKENDTYKIAIGARPQKARLALEASNILNKE
                     KDIDKASIVASKELTYGSNMRASKEYRKDMASALVKRMYNAIEEGMYND"
     gene            643..2949
                     /locus_tag="MJE33_003488"
     CDS             643..2949
                     /locus_tag="MJE33_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBY9649395.1"
                     /translation="MINKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDTVRFIGDE
                     VALVAAEDEKTALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     KNNIVSKEFFEFGDVEAKLKESDFVVESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRILRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDVKVGADKEGNLKAIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFNARTVYTNRLPAGALRGYGATQGIFAIDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTNGKAAEDEILSCELRQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKLLSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKDILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFASDDVPVVLEATESFGMLKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPIFDKD
                     GRLLTDSFLQYKVPTKKEVGNILVDFAENFDPGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRLRELPMTFEKVYMGMKNNNKQL"
     gene            3088..4365
                     /locus_tag="MJE33_003489"
     CDS             3088..4365
                     /locus_tag="MJE33_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBY9649396.1"
                     /translation="MSNRIKNIHYGTQRQQLKNLTKKEYQALREMCFLAKNLYNVGTY
                     NVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSSFRSFFGLIKLAK
                     EGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGILNVPMSPAFKKLYGKVSIKVPQN
                     ILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENLCTCVTNLG
                     DSFIIDGKRLKSVNQWANKQNDKIEALKLKKNITAITRKQFKVWNKRNNQVKDYVNKT
                     CFYIIDYCVKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKLEYLCKIKN
                     INFIRQEESYTSKSDFFADDFLPDINVDNVKKYDFKGERITRGQYKSSVGIIINADVN
                     ASLNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            4548..>5167
                     /gene="yqeC"
                     /locus_tag="MJE33_003490"
     CDS             4548..>5167
                     /gene="yqeC"
                     /locus_tag="MJE33_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HBY9649397.1"
                     /translation="MELSNIIEKNEIITVVGAGGKTSLINYFANFYRDKLKVLLTTTT
                     KIYVPNDYDNIIITIDGTVIPSICHGITVCGSYINNENKLVSIDSSILDEIVDQFDLV
                     LIEGDGSKRKKLKGWNAKEPVVYHKTTKTIGILDITSFGMNINEENIHRVEIFKKIAN
                     LDTSSINSSSTVSIENLKNIVLNPNGLFKNYSGKRVLFINKVENEK"
BASE COUNT         2101 a          563 c          979 g         1524 t
ORIGIN      
        1 aagatataaa tctaaattat ataaaagagg ataaaaaaaa tattttgata ggtgcagata
       61 cttcacttag aagtttagaa attgataaaa ctataaaaaa ttactgtagt ggaatacttt
      121 caagcgctgt ttctaatata gttggtgttc aatttagaca aggggctaga attggtgcaa
      181 gtgtattttc aaagtatggc ttttctgact taataccagc gcttttggtt gttggagcga
      241 aggtgagatt atacaataaa ggaatactag agttaagtga gtttttggat agtgaattaa
      301 gtagggatat attggtcgaa gtaatactac caaaaaaaga tgcaatagct gtttttgact
      361 caataagaaa atgcacagga gatttttctg tattaaattc agcaatgcta aaagaaaatg
      421 atacttacaa aatagcaatt ggggcaagac ctcaaaaagc tagattagca cttgaagcga
      481 gtaatatatt aaataaagaa aaagatatag ataaggcatc tattgttgct agtaaggaac
      541 ttacttatgg aagtaatatg agagctagta aagagtacag aaaggatatg gcaagtgcat
      601 tagttaaaag gatgtataat gctattgagg agggaatgta taatgattaa taaggggata
      661 agaaagatag atagtacagc aattgtcaca ggaaaacctc tatatactga agacctcatt
      721 atgcataaag atgttttgac attaaaacta cttagaagtc cacatgctca tgctaagata
      781 aagaatatag atacatcaaa agccgaaaaa attccagggg ttgtgggtat ttatacgtat
      841 aaagatgtac cacaggtcag atattgtgtg ccaggtcaaa cagaaccaga agcatcacct
      901 tatgatagag ttttacttga agatacagtt agatttattg gagatgaagt agctttagtg
      961 gctgctgaag atgagaaaac ggcacttaaa gccttaaaaa tgataaaggt agattatgag
     1021 gttttaaagc ctgtgtttga tgcaagagaa gcagaaggaa atgaagttgt tatacatcca
     1081 gaaaaagaca tagtagagcc atatcctttt ggattggatg caaaaaataa tatagtatca
     1141 aaagaatttt ttgaatttgg agatgttgaa gctaagctta aagaaagtga ttttgtggtt
     1201 gaatcttcat acaaaaccca aagtcaatcg cattgcatga tggaaactca taggtcatat
     1261 tcatataaag atataaatga tagaattgtt gtagtttcat cattacaatc accacacaat
     1321 gtaaagagaa tacttagaaa agtattgggt ataccatctt caaaaataag agtaattaaa
     1381 cctagaatag gtggtggatt tggaggaaag aatatagcag caacagatat atttactgca
     1441 tttgttacta tgaaaactgg tagaccagca aaatttgtat ttacaagaag tgaaactttt
     1501 gtaggttcta atagtagaca tgaaatgttt tttgatgtta aagttggtgc agataaagaa
     1561 ggaaatttaa aagcaataga gctaaagtca ttaaacaata caggtgctta tggtgggaat
     1621 ggtgtctctg tttcttccga atctggacac aataccttac caatatataa tgatattgat
     1681 gctgttagat ttaatgctag aacagtatat actaataggt taccagcagg agcacttaga
     1741 gggtatggtg ctacacaggg aatatttgca atagatagtg ctattgatga attggcacat
     1801 aagattggaa tggatccact tgaatttaga attaaaaata taatagatag gtataccaat
     1861 ggaaaagcag ctgaagacga aatattaagt tgtgagctga gacaatgtat agaaaagggc
     1921 aagaaattaa ttaattggga tgataaatat ccatgtaaag atataggaaa caacaaagta
     1981 agagcagtag gtatggcagt agcaaatcat ggttctggta taccgcgtgt agatatggca
     2041 acagtcacta taagaatgga tgaagatggc acatataaac tattatctgg tcttgctgac
     2101 ttaggtcaag gggctgatac aattcaagct caaattgctg cggaagcttt aaatactact
     2161 gtagatagaa tttctattta tacaggtgat tcagacttat gtccctatga tacaggagca
     2221 tttgcttctt gtacgacaac ggttacagga aatgcaacaa taaagacagc aaataaatta
     2281 aaagatatat tactttcagt tgcagagtca aagctaggat ttaaaaaaga agatttagag
     2341 ttgggaaaag acagggtttt tgttaaaaac gataatagca agtttgttct attagaggaa
     2401 ttgggtagag aatctgttgg aggaatagga tttgctagtg atgatgtacc agtagttttg
     2461 gaagcaacag aatcttttgg catgttgaaa aaaataaaac catttatagc aggatttgca
     2521 gagatagaat tagataaaaa tacaggcgaa tataaagtca taaattttgc atgtgtacct
     2581 gattgtggaa cagttataaa tccaacttta gctagaatac aagtagaagg tggtgtgatg
     2641 atgggtatag ggtttgcaat gtatgaagac cctatatttg ataaagatgg tagattatta
     2701 acagatagtt tcctacagta taaagttcct acaaaaaaag aagttggaaa tatattggta
     2761 gattttgctg agaatttcga cccaggaggt ccttatggtg ctaaatcatt aggagaaata
     2821 gttatacata caccagcacc agctatagca aatgcaatat ttaatgctac aggagttcgt
     2881 ttaagagagc ttcctatgac ttttgaaaaa gtttatatgg gtatgaaaaa taataataaa
     2941 caattataat aaataataag atgtataaaa gcaagtaaat ttaaattatt ttgtaaaata
     3001 aagtaattaa atggacatta tatatggtgt tttgtaaaaa taccgtatat aatgtctagt
     3061 agagtatatt ctaaaggagg tgagttcatg tcaaacagaa taaaaaatat acactatgga
     3121 actcagagac aacaattaaa aaacttaact aaaaaagaat atcaagctct acgagaaatg
     3181 tgcttcttag ctaagaactt atataatgtt ggtacatata atgtaagaca gcattatttc
     3241 aatcaaggag aacatcttaa ctatgaagct aattatcatg tatgcaaaga aaatgagaac
     3301 tataagcttc tcaatagtaa tatagcgcaa caaatattaa aagaagtaga cagttcgttt
     3361 aggtcatttt ttggattaat taaattagcc aaagaaggaa aatatgactt tagagggata
     3421 aaatcaccta aatatttaga taaagaagga ttttttagca taataatagg tcaaataaga
     3481 atcaaagaag atgggatttt aaatgtacct atgtctccag catttaaaaa actatatggg
     3541 aaagtaagta taaaagttcc acaaaatata ctagagaaaa aaattaaaga aataagaata
     3601 atacctaaac ataaagctag gttctttgaa attcagtatt gttatgaaat accaaaatca
     3661 gaggaagttt caaatcaaaa aaatgcacta gctatagact tgggtttaga aaatttatgt
     3721 acatgtgtta ccaatttagg tgatagtttt atcatagatg gaaaaaggct aaaatctgtg
     3781 aatcagtggg caaacaaaca gaatgataaa atagaagcct taaaattaaa aaagaatata
     3841 actgcaataa caagaaaaca atttaaagtt tggaataaga gaaataatca agttaaagac
     3901 tatgtaaata aaacctgttt ttatataatt gattattgtg taaaaaataa tattggaaat
     3961 ttaattgtag gatataatgc taagttagaa aaaagtgata atatggagag aaaaatatat
     4021 agaaatttta taagaatccc atttggagag ataaaaggaa aattagaata tctgtgtaaa
     4081 ataaaaaata tcaattttat taggcaagaa gaaagctata ctagtaaatc agattttttt
     4141 gctgatgatt ttctacctga tatcaatgtg gacaatgtta aaaaatatga ctttaaaggg
     4201 gaaagaataa cgcgtggaca gtacaaatcc agtgttggaa tcattataaa tgcagatgta
     4261 aatgcgagtc ttaatatact aaaaaaatca caagtagtta atttatctaa tttacagaat
     4321 aatcctcaaa tattaaagca accacagaga gtaagaatat cttaattata tttaaaagat
     4381 aaactgatag cgtaagctta cagcttagcg gcgaattgga tccgccttgt tgtttacaac
     4441 agaatctatt tactttcgag tttatggaat ttcaaaaatc acttttacta aaaaggtcta
     4501 actataataa ttaagagatt agaaaactgg ttataagagg tgaaagtgtg gaactatcaa
     4561 atataataga aaagaatgaa ataataactg tagttggagc aggaggaaaa acttcgctta
     4621 taaactattt tgcaaatttt tatagagata aattgaaagt tctccttaca acaacaacta
     4681 aaatatatgt accaaacgat tatgacaata taattattac aatagatgga actgttattc
     4741 catctatttg tcatggaata acagtctgtg gcagttatat aaataatgaa aataaattag
     4801 taagtattga ttcctctatt ttggatgaaa ttgtagacca atttgattta gttttaatag
     4861 agggagatgg ctcaaagaga aaaaaactta aaggttggaa tgctaaagaa ccagtagtat
     4921 atcataaaac tacaaaaacc atagggatat tagatataac atcatttgga atgaatatta
     4981 atgaagagaa tatacataga gtagaaatat ttaaaaaaat agcaaattta gatacaagta
     5041 gtattaatag tagttctaca gtaagtattg aaaacttaaa aaatatagta ttaaatccaa
     5101 atggattatt taaaaattat tctggaaaaa gagtattatt tattaataaa gttgaaaatg
     5161 aaaaata
//
LOCUS       DAGSRM010000150         5101 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.156, whole genome shotgun sequence.
ACCESSION   DAGSRM010000150 DAGSRM010000000
VERSION     DAGSRM010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5101
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.156"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            73..1536
                     /locus_tag="MJE33_003491"
     CDS             73..1536
                     /locus_tag="MJE33_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBY9649398.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSLKLKFFQIRDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHDGQYRGGPPYYFEKGLGWKKCSVLFSIVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVASSIALAIIIFGGIKRIGLFAEKVVPIMGGAYLIMTL
                     VIIITNISLVPEAIVTMFKSAFGFGSVFGGMLGSAISWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIVITGMYNVTGVCTALPGVEPGPQY
                     VQSAINTLHTQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKHEKYIIN
                     VCRFMLIACAAMCCFIEPLTSWALGDICIGVLTYINLIAIFTLKKPAIKAYYDYIRQK
                     KMGIKRSDRTFNPIELDIRNAEFWENRYLKEDKHELN"
     gene            1592..2728
                     /locus_tag="MJE33_003492"
     CDS             1592..2728
                     /locus_tag="MJE33_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBY9649399.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKISEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVTPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLVDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLVDIFTKIMEEFGIYEDEQVMLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINSYAKDRVIDGIKILALTGFNLFENPELLKKMKEE"
     gene            2964..4079
                     /locus_tag="MJE33_003493"
     CDS             2964..4079
                     /locus_tag="MJE33_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HBY9649400.1"
                     /translation="METLEVRNIELSGTNYEIGYRLGELVANMPEIIEGQINKSNLVS
                     KKEEKEMIELFDKYCPGLNEELQGFADAIQVNCNQILYYTMTYLKPGCSQVALAPELT
                     ENGHVLFARNFDFSHNMEDFVLCKTKVNGKYAHIGTTIMQFGRGEGMNECGLGVSQSS
                     CGIPVGNSDGLRKPAIVGLQFWAVIRYLLENCKDVDEALEYLKDMPIAYNINLLLADK
                     SGNIALVETLDGKKEVNIINSLESKREYFLHSTNHTHIDKLHKLDPQSMKNSIHRYKL
                     IKEYINKSKKIGEKELMNLLSSKYPNGLSCNYYNDFFGTLKSIVMDLNIGKFNILWGG
                     LENKWESYYLKNDIKSITQRININIEKAPSDFFDFIE"
     gene            4231..5100
                     /locus_tag="MJE33_003494"
     CDS             4231..5100
                     /locus_tag="MJE33_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBY9649401.1"
                     /translation="MDYLKYFEKAVMYIENNLHENITVNDVAKETGYSYYHLTRLFKS
                     MFGESVGSYIKKRRLVSSTKELLYSDKKVIDIAIGSGFESSEAFSRAFKSIYKVSPIE
                     YRKNRIDVFVGKKKKLELDFMRHLVGNITIKPVIKEIEKIKVIGIKDKVILENNSLPD
                     LWEKFRKVHHIVPNTLPSKRVFGICEAISEIHLVSESMEFNEIIGLEVNSYDVIPNSF
                     VSKTIKEGKYAIFTHTGSLDNLDKTYEYIWGTWLLSSKEELDIRDDFEVYDERFLGPS
                     NVNSQIDIYIPIK"
BASE COUNT         1950 a          536 c          974 g         1641 t
ORIGIN      
        1 attatgtaaa atattgaaat atagctgttt aaattattgt ctataaatat aaaatgaatg
       61 gtggtgagtt aagtgaccga tttagttaac accattaatg gaattgtttg gtcacccgta
      121 ttagttgcaa tgtgtttgtg tataggatta tatttttcat tgaaattaaa attttttcaa
      181 ataagagatg taaaagaaat gtttactctc ttgcttgaag gagatagttc tcatgttggt
      241 atatcatcat ttcaaggttt tgctacatct atggctggaa gaatagggac tggaaatata
      301 gctggtgtag ctgtagcaat agcaatggga ggtccaggag cattagtatg gatgtgcata
      361 atggctacat taggttcagc aactgcattt gtagaatcta ctctagctca aatatataaa
      421 gatgagcatg atgggcagta tagaggagga cctccatatt attttgaaaa aggtcttggg
      481 tggaaaaagt gttcagtttt attttcaata gttgcaataa taagttatac attttttatg
      541 cctggtacac aatcaaacac tttatatcta gcaatgaatc aagcatttgg attttcaaaa
      601 atgacaatag cagtagcatc ttctattgca ttagcgataa taatttttgg aggaattaaa
      661 aggattggtt tatttgctga aaaagtagta cctataatgg gtggagcata ccttattatg
      721 acattagtaa taattataac aaatattagt ttagttccag aggctattgt aacaatgttt
      781 aaatctgcat ttggatttgg ttcagttttt ggaggaatgc taggttcagc aatatcttgg
      841 ggagtaaaaa gaggtatata ctcaaatgaa gcaggagaag gttctgggac ttatggtgcg
      901 gcagcagcag aagtaagtca tccagccaaa caaggattag ttcaagcatt ttctgtttat
      961 gtagacacat tattaatatg tatggctaca ggtattatga ttgtaataac aggaatgtat
     1021 aatgttacag gtgtttgcac agcactacca ggtgtagaac cgggaccaca atatgtacag
     1081 agtgcaataa atacattaca tacacaatta ggtgatattt ttatagcaat agttttatta
     1141 ttatttacat ttacaacttt attagccttc tcatatatga tggagactaa tgtatcttac
     1201 ttagtaacta gaattaaagc agaaaaacat gaaaagtaca taattaatgt ctgtagattt
     1261 atgcttatag catgtgcagc aatgtgttgt tttatagaac cacttacaag ctgggcatta
     1321 ggtgatatat gtataggtgt attaacatat ataaatttaa ttgccatctt tacactaaaa
     1381 aaaccagcta taaaggcata ttatgattat ataagacaga agaagatggg aataaaaagg
     1441 agtgatagaa cttttaaccc tatagagtta gatataagaa atgctgagtt ttgggaaaat
     1501 agatacttaa aagaggataa gcatgagtta aattaaagtt ataataaaat ataaaaatta
     1561 ctaaataaat ttagtgggag gtaagattgg tatgaaagaa agaataatac aagtggctga
     1621 ttcaaagaaa gaaaaaattc tagggctatg tcaatcttta tatgatgaac cagaaattgc
     1681 tttgcaagaa tataaatctg ctaaaaaaat atcagaattt ttaagagaag agggttttga
     1741 tgttgaggaa aacttggcag gtatggctac agcttttaaa gcaaccaaga aaaatggaga
     1801 tggtcctaaa atagcattta tagcagaata tgatgctctt ccaggcaatg gacatgcatg
     1861 tggacatcat ttaattgcat ctatgggagt tggagctgga atagctcttt caagtatact
     1921 ggatacttac aagggagaag taagtataat tggaacacct gctgaagaaa ctggagatgg
     1981 aaagccttac ttgatagaac atggtgtatt tgatggctat gatgcagcaa tgatgataca
     2041 tcctaattca aaaacttgtg taacacctga aattatagca ataggaggtc tagatttcat
     2101 atttactgga aaagcatctc atgcaggagc taagccatat aacggaataa atgctttaga
     2161 tgcagtagta ctactatata ataatataaa tgcactaaga caacaattag tagatggaac
     2221 gagaatacat ggtattatac tagaagcagg aactgctgca aatgtaatac ctgatatggg
     2281 aaaagtaaga ttagagataa gggcaaaaga acaaaattac tttgatgaag tagtagaaaa
     2341 agtgaaaaat tgtgctagag gtgctgcaat agccactgga tgtgaacttg aattttatca
     2401 ctttgaacca acttgccaag gattaaatga aaataaagtt ttggtagaca tatttacaaa
     2461 gattatggag gaatttggaa tatatgaaga tgaacaagta atgttaggtt caacagatat
     2521 gggaaattta agtcaaataa tgccatgtat acatcctttg atgaagtttt ctgaaaatgg
     2581 agaagaacta catacaaaag aatttttgga ggcttctata aattcatatg caaaagatag
     2641 agtaattgat ggtataaaaa tattagcttt aacaggattt aatttatttg aaaatccaga
     2701 gttattgaaa aaaatgaagg aagagtagaa cttagactat ttttaattat actctttaaa
     2761 ataagaagag tgtctcaaaa ggaacttttt agtttatgag acactctttt ttgtatgaaa
     2821 ttaaatatat ttttattatt ttaaacatga aaaagaggct tatctgtttt tatatgacct
     2881 tttttaactt gtagcacaaa atatcatgaa tgattacaat tcgaatgata aaatcgatta
     2941 taagatatat aggaggggta catgtggaaa ctttagaagt aagaaatatt gaattgagtg
     3001 gtacaaatta tgagatagga tacagactag gagaattggt tgctaatatg ccagagataa
     3061 tagaaggtca gattaataaa tctaatctgg taagcaaaaa agaagaaaaa gagatgatag
     3121 agttgtttga taaatattgt ccaggtctta atgaagaatt gcaaggtttt gctgatgcaa
     3181 ttcaagtgaa ttgtaatcaa attttatatt atactatgac ttatcttaaa ccaggttgta
     3241 gtcaagttgc attagcacca gaattaactg aaaatgggca tgtactattt gcacgtaatt
     3301 tcgatttctc acacaatatg gaggattttg ttttatgtaa aactaaggta aatggaaaat
     3361 atgcacatat aggtactacc attatgcaat ttggaagagg tgaaggtatg aatgaatgtg
     3421 gcttaggtgt aagtcagtca tcatgtggta ttcctgtagg caattcagat ggtttaagaa
     3481 aaccagcaat tgtaggttta cagttttggg ctgttattag atatttgtta gaaaattgca
     3541 aagatgtaga tgaagcatta gaatatttaa aagatatgcc aattgcatac aatattaatt
     3601 tattgcttgc agataagtct ggaaacattg cacttgtgga gactttagat gggaaaaaag
     3661 aagtaaatat aatcaacagt ttagaaagta aaagagaata ttttttacac tctactaatc
     3721 atacacatat tgataaactt cataaattag atccacaatc aatgaaaaat tctattcata
     3781 gatataaatt aattaaagaa tatataaata agtctaaaaa aattggagag aaagagttaa
     3841 tgaatttatt gtcttcaaaa tatccaaatg gcttatcttg taattactat aatgattttt
     3901 ttggaacttt aaaaagtata gttatggatt taaatatagg aaagtttaat attttatggg
     3961 gtggactaga gaataaatgg gagagctact atttaaaaaa tgacattaaa tctataacac
     4021 agaggataaa cataaatatt gaaaaagcac catctgactt ttttgatttc attgaataaa
     4081 aaatgtattc aattattcta aataattagg atatataaat caactaaaag tttttattta
     4141 tcgtattgaa ttagttataa tattttagta tagtaaaaaa tagtgctatt ttacatattt
     4201 aaagagttta agtagaagag aggtgccata ttggattact taaagtattt tgaaaaagct
     4261 gtaatgtata ttgagaataa tttacatgaa aatataactg taaatgatgt ggcaaaagaa
     4321 actggatatt cttattatca tttaacacgc ttatttaagt ctatgtttgg tgagagtgta
     4381 ggaagttata taaaaaaaag acgattagta agtagtacaa aagaactttt atatagtgat
     4441 aaaaaagtga tagatatagc tattggtagt ggatttgaat cttcagaagc atttagcaga
     4501 gcatttaaat ctatatacaa ggttagccct atagaatata gaaaaaatag aattgatgta
     4561 tttgttggta aaaagaaaaa attagagtta gattttatga gacatcttgt tggaaatata
     4621 acgattaaac cagttataaa agaaatagaa aaaataaagg taataggtat aaaggataaa
     4681 gtaattttag aaaataattc attacctgac ttgtgggaga aattcagaaa agtacatcat
     4741 attgtcccaa atactttgcc atcaaagaga gtttttggaa tctgtgaagc aatctctgaa
     4801 atacacctag ttagtgaaag tatggagttt aatgagataa taggacttga agttaatagt
     4861 tatgatgtga ttccaaattc atttgtttca aaaactatta aggaaggaaa gtatgctata
     4921 tttactcata ctggtagctt agataattta gacaaaactt atgaatatat atggggtact
     4981 tggcttttaa gttcaaaaga agaacttgat ataagagatg attttgaagt atatgatgaa
     5041 cgatttttag gtcctagtaa tgtgaactca caaattgata tttatatacc tataaaataa
     5101 t
//
LOCUS       DAGSRM010000151         5049 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.157, whole genome shotgun sequence.
ACCESSION   DAGSRM010000151 DAGSRM010000000
VERSION     DAGSRM010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.157"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(542..2677)
                     /gene="rnr"
                     /locus_tag="MJE33_003495"
     CDS             complement(542..2677)
                     /gene="rnr"
                     /locus_tag="MJE33_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBY9649402.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIVSPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDNIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLP
                     NGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKLKKTFEHVV
                     EDFKNAEILARILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYIYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
     gene            3273..3887
                     /locus_tag="MJE33_003496"
     CDS             3273..3887
                     /locus_tag="MJE33_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBY9649403.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYCSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTSRDYFKSNPDVAISLLSSLKEDDSEYLRKS
                     VGNALRDISKKYPDLVKKELDSWDISNKKVEFVYKLASKCILAK"
     gene            3905..4267
                     /locus_tag="MJE33_003497"
     CDS             3905..4267
                     /locus_tag="MJE33_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY9649404.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYEGYPLKVEYSLTPIRGKQILDALAIMQDIGIS
                     FMLDNGMEDILKEKGVLK"
     gene            4373..4870
                     /locus_tag="MJE33_003498"
     CDS             4373..4870
                     /locus_tag="MJE33_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HBY9649405.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELNENESVTATDFVQGTTSWHGKLGG
                     LDRLEASVYGGMAPPDRPHVYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
BASE COUNT         1637 a          775 c          608 g         2029 t
ORIGIN      
        1 acatttctta attatttatt tttcaattag ccacttctat taagcatttc ttaattattt
       61 atttttcaat tagccacttc tattaagcat ttcttaatta cttatttttc aattagccac
      121 ttctattaag catttctcaa ttacttattt ttcaattagc cacttctatt aagcatttct
      181 caattactta tttttcaatt agccacttct attaagcatt tcttaattac ttatttttca
      241 attagccact tctattaaac atttcttaat tatttatttt tcagttggct ttttctatta
      301 aatgtttctt aattacttat ctttcagtta gctctttcta ttaaacattt ctcaattact
      361 tatttttcaa ttagctactt tcattaaata tttcttaatt atttatcttt cagttaacta
      421 tttccattag atatttctca attacctatt tttatcattt attttataga gtattgtgca
      481 ctatatattt atacatatca taaatactac aacttgttat ctcttatttt agctgtttta
      541 tctactcctc tattttttca actatctcaa agtcaatttc tttaaaatct acattaacac
      601 tttcaacctt tatcttaaca aggtctccta tcttatatga cttcttagtt ctttctccta
      661 aaacactata agaactctca tcataaatat agtaatcatc attcatattt gcaagtctta
      721 tcaatccttc tatagtatta tctaactcaa caaatattcc aaatgaagtt acgctagata
      781 caacaccctc aaattcttga ccaactttat ccttcatata aagagcctta tagaaatcat
      841 gtacatctct ttcagctaac tctgcctttc tttctttctc ggatgattga acagatgcat
      901 aatccactat agtagtaagc tgttcttgtc tttttccact tattttatta tttaaagatt
      961 cttttattat tctatgaatt tgaaggtcag gatatcttct tattggtgaa gtaaagtgac
     1021 agtagaattt agctgctaat ccaaaatgtc cactacattc tggtgagtac ttagcttgtt
     1081 ttaaagaacg aagcattata gtactgatag ctccttcttc ttttttgccc tttattttct
     1141 ctattatacc ttgtaatgct tttggatgta cttcctcttt atcacctttt attatatatc
     1201 caaatgtaga tataaactta ctcaattctt ccattttttc caaagatggt gtctcatgta
     1261 ttctatacac aaacggtatt cccatccagt aaaaatgttc ggcaattgtc tcattactta
     1321 taagcataaa ttcttctatt atcttattag atattcttct ttcatatggc tttatatcta
     1381 caacttcgcc ttttccattt agaattatct ttgcctctgg aaaattgaag tctatagctc
     1441 ctcttttttc ccttcttgac ataagaatcc tagctaaaat ttcagcattt ttaaaatctt
     1501 caacaacatg ttcaaaagtc tttttaagtt tttcatcatc tttttctaat atatcagata
     1561 cttctgtata agtcattcta gcttttgaat ttattacaga ttcacatatt tcatgtttaa
     1621 ccacatttcc tttatggtct atttccataa atatagaaag agttaactta tcttcaaatg
     1681 gatttaagct acatactcca tttgaaagag tttttggcaa cattggaatt actttatcta
     1741 ctaaataaac tgatgtagct ctttttaagg cttccttgtc aagtttattt ttctctctta
     1801 cataatgtgt aacatcagct atgtgaactc ctagtttgaa gtttccatta ggtaatactt
     1861 ctattgatat ggcatcatct aagtcttttg cgtcatctcc atctatagta aatatgttta
     1921 aatctcttaa atctcttctt ctttttatct cttcttgagg aatctcaaca gctacagctt
     1981 ctgcttcttg caatactttc tttggaaatt cttctggaag accatgttct cttattatag
     2041 aatctatttc tacgcctctt tcaccttttt gacctagtat ctctataatt ttgccttccg
     2101 gttttctatc ctcttgtggc catactgtaa tttcacatac taccttatca ttatctactg
     2161 ctccactaaa atgtttttta ggtatatata tatctttagt aaatttcttg ttgtctggtg
     2221 ttacaaatcc aaaagtttta cttgattgga aggtacctac tattctagtt atttctctct
     2281 ttattacttt tactattctt ccttctgctc tcttttcttc agttgcaggt actactattt
     2341 ctgccataac tctatcatta tgcatagcac catttatatt gtcacttgag ataaataaat
     2401 cctgtgtata ttcaacatca gattctacaa atccaaaacc ctttttgtgt gatacaaatt
     2461 ttccaacaaa gtatcccatt tgattgggag atactatttt tcctttttta gtaaagcaga
     2521 tataaccatc ttcttctaat tcatttaaaa aattataaaa cattggcatt tctgatgaat
     2581 gtatatcaaa tatttctgct aattcttctt tttttagagg attatatgca ctttcattta
     2641 ttaatcctag caaactttct ttaagtcctg gtatcatttt tccctcctgt tttacttttc
     2701 attatattat tatttatatt gtcttttata tttcttcaga caaaacattt tttacttatc
     2761 acataatttt ctatatttcc ttattattat tacaagttaa aattacttat ttatattttt
     2821 gatttaatgt aagctttgtc atactaactt caaacaaatt attatgtttt ataaattatt
     2881 tgtaaatatc attataatta cttaagttat tctttatcat aattgcttaa attactcttt
     2941 atcacctaat tatacaccat caaataggat tggtaaaata ttcatctcat ataagtatta
     3001 ctatataata atttattcta ttagtatact caaatcatgt aaaatttata taactcaatt
     3061 aattttttaa atacaacttt ataatataaa acttataata aaagtaaatt tatatgttaa
     3121 atgaggttga ttttttatta ttttttattt atgctagtac taaagacttg caaacgtttt
     3181 ttgacaagtc tttattttgt ctaaattaaa caaacttttt tacaaataaa tacataaaat
     3241 ttaaactaat ctatttattg gaggagataa aattggaaga agtaattgca aaaattatta
     3301 ctatagaaaa tggattcaaa gaaattgaaa acattgctaa aaaaattgta aaaaataatg
     3361 actcaaaaaa gtgttattat ctatcaatct acttatatag gtcagaatat tatcaagtaa
     3421 gagaattggc tgtgtttata cttggtttta tatcagtaac tatttctgaa gctttgttat
     3481 ttttaaaaga taatgtgagt aaagatgaaa actggagagt acaagaaata ctagctaaat
     3541 catttgatta ttattgcagt gaagtaggat atgaaaaggc tcttcctgtt ataaaagaat
     3601 ggcttaattc tgatagtcca aacataaaaa gggctgtgac agaaggtctt cgaatttgga
     3661 ctagtagaga ctattttaaa tctaatcctg acgttgctat atcactactt tctagtttaa
     3721 aagaagatga tagcgaatac ttgagaaagt ctgtaggtaa tgctcttaga gatataagta
     3781 aaaaatatcc agatttagta aaaaaagaat tagattcatg ggatatatca aataaaaaag
     3841 ttgagtttgt atataagctt gcttcaaaat gtatcttggc aaagtaaaat ggaggtgaac
     3901 ttatttgaaa cttcgtgatg agtatacttg cccattagaa ctaacacatg atatgataaa
     3961 aggcaaatgg aaatctatta tattatggag attacgcata ggaccaactt ccttatcaaa
     4021 acttgaaaag gaaatcaatg gtattaatca aaagatgtta cttgaacaac ttaaagaatt
     4081 aattgaattt ggtcttgtag ataaaaagac atatgaaggt tacccactta aagtcgaata
     4141 ctcacttaca cctattcgag ggaaacaaat attggatgct ttggctataa tgcaagatat
     4201 aggaattagc tttatgttgg ataatggtat ggaagatatt cttaaggaaa aaggtgtttt
     4261 aaagtaaata gatacccaca aaaaagtaag taatttacca ttattttcaa ctggtatata
     4321 attaaattat agatatattc tattaattta ttataatagg aggtaaatta ttatgaaagt
     4381 aactagtaca ggtatagtaa atggtgttat agaagataaa tacggtaaaa gaggaactca
     4441 gttcaatgaa ggtggtatgc ctacctattc actacctcta aagtttgaag atgctccaaa
     4501 gaatacagta tcctttgctg tatttttaga agataaagac tctgttcctg tatgtgggtt
     4561 tgcatggata cattggttag ctgtaaatat aacaaaagat gagttaaatg aaaatgaaag
     4621 tgtaacagct actgattttg tacaagggac tactagttgg catggtaaac taggaggtct
     4681 tgacagatta gaggccagtg tatatggtgg aatggctcct ccagacagac ctcatgtcta
     4741 tgaaatacat gtatttgcac ttgatactat ccttgacctt gaaaaaggat tctatatgaa
     4801 tgaatttttc aaagctattg aagggcatgt tttagataca tttacactaa aaggaagtta
     4861 ttctaattaa ttaggttgac tatagctaat cattaattaa atgctacatt taattgatga
     4921 ttagcttaat tgttattaga atattttctg cactttatct taatatatat tttagttgac
     4981 tggtatttta atttatatta gattattatc aatttatata aattgatttt agcttaaaac
     5041 ttacttatc
//
LOCUS       DAGSRM010000152         4964 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.158, whole genome shotgun sequence.
ACCESSION   DAGSRM010000152 DAGSRM010000000
VERSION     DAGSRM010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4964
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.158"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(141..1733)
                     /gene="hcp"
                     /locus_tag="MJE33_003499"
                     /gene_synonym="priS"
     CDS             complement(141..1733)
                     /gene="hcp"
                     /locus_tag="MJE33_003499"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBY9649406.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGGTANLQDELTGVLIGL
                     SRATIGNKNRPTSETDKIMIEGLFTTITNVSFDDEAIKRQIEKTEVEKSKLVPRCSDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     GGYREDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGAIRHFLLVGGCDGAKPG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDIGGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMVLSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
     gene            complement(1793..2518)
                     /locus_tag="MJE33_003500"
     CDS             complement(1793..2518)
                     /locus_tag="MJE33_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBY9649407.1"
                     /translation="MMRKIIKIDEEKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKAQKKTLACGCPGSQSKSINRE
                     VSNSTSISNDIVEIKGSQLNQWPVQIKLVPTNASYLKNASLLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEVDYAEKLTEILKENDIKNIVVTRMEVPCCGGIVNAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            2659..3387
                     /locus_tag="MJE33_003501"
     CDS             2659..3387
                     /locus_tag="MJE33_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBY9649408.1"
                     /translation="MKTNYRLHMQQGSDYLKKFLEKHIDLLKKIPLFEGIKSDELEEM
                     LECLGVVDKTYPKDSIIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLF
                     GEVFACTRLHKSNVTVTTTSSCEVLFIKFKSVTGICSSACVFHNRLVENMLQLIAEKN
                     ILLHNKIELLSRKTTREKLLMYFSKQIEQTGSHQFTIPFSRNEMADFLCVDRSSMSRE
                     LGKMRDEGLLSFNKNKFEIFYFQK"
     gene            complement(3551..4822)
                     /locus_tag="MJE33_003502"
                     /pseudo
     CDS             complement(3551..4822)
                     /locus_tag="MJE33_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416360.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
BASE COUNT         1618 a          888 c          608 g         1850 t
ORIGIN      
        1 ttcaattatt acaaaaaata aataaagggt catctcaaaa ttgatttttt aatcaaattt
       61 taagtttaac cccttatctt aattgtggca tacttaaaaa tattttaact ataaatatct
      121 ttaagtatag atatatttat ttatcctaaa atcttcttca tatcttcttc aggagtagat
      181 ataggtgtca atgcaaattt atcaaccaat atatctaata cattcttaga taagaatgct
      241 ggtaaagaag gccctatata gatatttttg atacctaaat gaagtagtgt aagaagtata
      301 caaacagctt tctgctcata ccatgacagt accatgctaa gaggcaaatc atttactcca
      361 cattcaaaag catttgccaa tgaaactgcc actttaattg cactataagc atcattacat
      421 tgtcccatat ccattaaacg aggtaagcca cctatatccc caaggtctaa atcattaaat
      481 ctatattttc cacatgcaag tgtcaatata acagtgtctt taggagcttg ttttacaaat
      541 tcagtgtaat aatttcttcc aggttttgca ccatcacaac ctccaaccaa taaaaagtgt
      601 cttatagctc cactctttac agcatctact actttatctt gtacagatga aactgtacct
      661 cttgcaaaac cagtagttaa tgtatctcct ccattaatac cagtcatatg aacatcttct
      721 ctatatcctc caagttcaag agctttttct attacactag aaaaatcttt atcttcatca
      781 atatgaacca tacctggata agcaactact tctgtagtaa atactctatc tctataacta
      841 tctttaacag gcattaaaca gtttgttgta aataatattg gagcaggaat attatcaaat
      901 tctttttgct ggttttgcca tgctgtacca aaattgcctt ttagatgttt atattctttt
      961 agttttggat atgcatgtgc tggtaataac tcaccatgag tatatatatt tataccctta
     1021 tctttcgttt gttctaataa tttgtgtaaa tcataaaggt catgtcctgt aactacaata
     1081 aatggccctt tctcgatttt catagatacc tgagtcggtt ctggtgttcc ataagtttca
     1141 gtatttgctc tatcaagcaa ttccatacat tttaagttta tttctccagt tttcataact
     1201 agacctaaaa gttcgtcaat agacaagtct cctccaactg ctattaatcc ttcatagaaa
     1261 aattcattta cgtctttgtc tgtataacca agtaccattg catgatatgc ataggcagcc
     1321 atacctctta atccaaataa gattaatgat tttaatgaac gtatgtcctc attatcattc
     1381 cataaatcct tcatatcata gttatcattt ctattacaag ttgttgaaca atctgaacat
     1441 ctaggtacaa gtttactttt ttctacctca gttttttcaa tttgtctttt tatagcttca
     1501 tcatcaaaac ttacattcgt tatagtagtg aatagcccct ctatcataat tttatctgtt
     1561 tcacttgttg gtctattctt atttccaata gttgctcttg aaagaccaat caacactcct
     1621 gtaagttcgt cttgtaagtt agctgtaccc ccctttttac cacagacacc atttatagta
     1681 caacctttac ctcctgctgt ttgttcacat tgaaaacaaa acattttttc cattttttat
     1741 tcctcccttt ttatttatta aaatttatta tatttttcta taattcactt aattaaattt
     1801 ctccatctac aatttttcca tctacagata tagtaactat ttgccatggt atcatttttc
     1861 cactattttg tagagctgtc ttaacagcat ttactatacc accacagcaa ggaacttcca
     1921 tacgtgttac tactatattt ttaatatcat tttcttttaa aatctctgta agtttttcag
     1981 catagtcaac ttcatcaagt ttagggcatc cgattaatgt aaccttattt ttcataaatt
     2041 tattatgaaa atttccatat gcatacgctg tacagtctgc tgctattaaa agacttgcat
     2101 tctttaaata tgatgcatta gttggtacta attttatttg tactggccat tgattaagtt
     2161 gagaaccttt tatctctact atatcattgc ttatagacgt actatttgaa acctctctat
     2221 ttattgattt agactgagaa cctggacatc cacatgctaa agttttcttt tgtgctttct
     2281 ttgcttccat atttgctttt actgcagctt catcatattc tgctgcttct ctatactcaa
     2341 atgaaatcgc atttgttgga caagttggca gacaatctcc cagcccatca caatagtcat
     2401 ctcttaaaag ttttgctttt ccatttacca ttcctatagc ttcttcatga caagcttcaa
     2461 cacatagtcc acaaccatta cacttttcct catcaatttt aattattttt ctcatcattt
     2521 atatcacctc ataaaatttt tttaatttaa agtattgatt tcttatatat ttatttttaa
     2581 tacctatatt tctttatttc aaatttcttg tagtaggatt tttatatccc cttgctttta
     2641 taaacttagt atagtacaat gaaaacaaat tatcggttgc atatgcaaca aggaagtgat
     2701 tatttgaaaa aatttttaga aaagcatata gacctactta aaaaaattcc tctatttgaa
     2761 ggaattaaaa gtgatgaact tgaagaaatg ttggaatgtt taggtgttgt agataagact
     2821 tacccaaaag atagtataat attctctgtt ggaagtgaag ttacttctat tggaattatg
     2881 cttaaaggaa gtgctcatat aataaaggaa gatatcgaag ggaataggaa tattgtagct
     2941 gaactcttgc ctggagattt atttggagag gtattcgcat gtactagact acataaaagt
     3001 aatgttacag taaccacaac atcttcttgc gaagttttat ttataaagtt taaaagtgta
     3061 actggtattt gttcatctgc atgtgtattt cataataggc ttgttgaaaa tatgctacaa
     3121 ttaatagctg aaaaaaacat tttacttcac aataaaatag aattactttc tcgtaaaaca
     3181 actcgcgaga aattgcttat gtatttttca aaacaaatag aacaaacagg tagccaccag
     3241 tttacaattc ccttttcaag aaatgaaatg gctgattttt tatgtgttga cagaagttct
     3301 atgtcaagag agcttggcaa gatgcgagat gaaggcttgt tatcttttaa caaaaataaa
     3361 tttgaaatct tttattttca aaaataaaat agatatttaa acatatttat ttcaatatat
     3421 actatattag ttgattcaaa ttataaatat catatttgaa tcttaaattt atatttttac
     3481 ttaaaaagtt aataggtatc taatttatta aaattaagat acctattaac tttattaatt
     3541 aagtgtacaa ttagcttatt ttctttgtag taattgctgt tttatctata agccaatctt
     3601 ttccttctac taatcttgaa actaacattg atgatactgt atttccagtt gtatttaata
     3661 tagtattagg aatatcagtt atagtagtta taactgttaa cagtggaatt gcctccactg
     3721 gaaaaccgaa gatgctaacc acgagcatct caccaataag tcagccacct gctacaggag
     3781 ccataaatac tccaacaaca aatacagtta taaataattg acctatcgtt gctggatttg
     3841 ccattccact acctaaaata cctgataaaa atgccacttt tattattact gctaaaactg
     3901 ccccatcttt atgtatattt attccaagag gtataactgt ttctgatata tcatcaggaa
     3961 ctcctatatt tttactagct tctaaatttg atggtataca tgctgaactt gaagaagtag
     4021 ctagtgccgt tgctattggt tttatagcat ttctccagaa ttgtctaaac ccttgttttc
     4081 ctgcagataa aaaagcatac agactgtaaa ataataagaa atacatggct gctgttagtg
     4141 aataaattac aaacaccctt acatatcctt gaattatttt acttccaagt tccccaatta
     4201 ttgtagctgt ataacagcct agccctatag gagcataaag catgaatata cttactactt
     4261 ttaatattgc ttccgttcct tctgataaaa gctttcttat tgtatttccc ctatctccca
     4321 tcatagctat tgctgttcca attaaaactg caaatataac taattgtagt aaattactac
     4381 tggaaaataa atctccaaaa tttttaactg taatacttga tactacttgg tctaataacg
     4441 ttccttttga ttctaaagtt tctgcatctt gagccaccat taaatttttt acacttgata
     4501 catctaatcc tttagacgga ttaaataata atgcagatgt aatacctaaa aatgcaatta
     4561 caagtgttgt tcccataaat actatagctg tacccttcat tatttttcca aatcgttcca
     4621 tattttttat actagcaaca cctgaagaaa cactaagaaa aactaatggt actactatca
     4681 ttaatagagc atttatgaat aaatctccaa atggttttag tattgaagct tttggaccaa
     4741 gtactaatcc tactatcgcc cctactatag tacatgctaa aagtataatt gatgacttat
     4801 aattttttaa aaagtccctc atttatattt tcctttctaa tgctttatat tataaattac
     4861 cccaaatgct aaaaaaatta aagcaaatta tctaatgcgc attaataatt tttgtaattt
     4921 tccccaacag ctactttagc aaatactgga atatatgtca attt
//
LOCUS       DAGSRM010000153         4963 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.159, whole genome shotgun sequence.
ACCESSION   DAGSRM010000153 DAGSRM010000000
VERSION     DAGSRM010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4963
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.159"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            558..788
                     /locus_tag="MJE33_003503"
     CDS             558..788
                     /locus_tag="MJE33_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBY9649409.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            781..1362
                     /locus_tag="MJE33_003504"
     CDS             781..1362
                     /locus_tag="MJE33_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBY9649410.1"
                     /translation="MNNKKHWYDYLWIFSSIYLILGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMILFKPRSWCVYCPMGTMTQMICKAKSKSV"
     gene            complement(1940..2617)
                     /locus_tag="MJE33_003505"
     CDS             complement(1940..2617)
                     /locus_tag="MJE33_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBY9649411.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSHYKKGNPVH
                     CGDSDCIGILIIKSGTVRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQISSSVFAKLSTENIHAELFSYKLATERFSDVMWAMQQILFMSFDKRLAS
                     FLIDEIAKNGSNTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
     gene            complement(2806..4170)
                     /locus_tag="MJE33_003506"
     CDS             complement(2806..4170)
                     /locus_tag="MJE33_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBY9649412.1"
                     /translation="MKESDKRIELLGSAPISKALLSMGLPTMIGMMITALYNLVDAYF
                     VGGLGTSQMGAISVAFPLGQLVVGLGLLFGNGAASYIARLLGHGDNEKAGKVVSTSIY
                     SSIIVGTLVIIVIMIFLNPILKLLGATKSIMPYAISYASIYVVSSIFNIFNVTMTNIV
                     TSEGAAKTTMCAMLLGALLNMILDPIFIYTFGLGVRGASIATAISQIASTLVYVGYIL
                     SKKSIFSFRINECCFSKEIFSEILKIGIPTLIFQLLTSLAITLTNMEATKYGDSVIAG
                     MGAVTRITSLGSLVVFGFIKGFQPIAGYNFGSKNYDRLHIAIKTSILWTTVFCVVLGL
                     ILILFPKSIISQFTSGDILLIENGSKALRANGFSFMLFGFYTVYSTLFLALGKAKECG
                     FLGMCRQGVCFIPIILIIPVFYGLNGILYAQPIADLMAAIIAGFMSIRLHKELNLLKI
                     NGMH"
     gene            4298..4819
                     /locus_tag="MJE33_003507"
     CDS             4298..4819
                     /locus_tag="MJE33_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBY9649413.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKAVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSKPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEENISIKTEVPETAKAVLEQQLVLAQAL
                     ETWINSLKDNMGN"
BASE COUNT         1878 a          646 c          792 g         1647 t
ORIGIN      
        1 gtttaacttg tggttaatag agtaagaatg taaatagaga taaacttcaa tatgagctta
       61 acttgtgatt aatagagtaa gagtgtaaat agagataaac ttcaatatga gcttaaccta
      121 tgattaatag agtaagaatg taaatagaga taaacttcaa tatgggttta acctgtggtt
      181 aatagagtag ggtgtaacta aagataaact tcaatatgag tttaacttgt ggctaataga
      241 ataagattgt gaataaagtt atagttttag atgggtatta acctgttact aatagaatat
      301 gattaggtgt aaaattatag agctgtataa gaaaatatta tagatatatt aaatattaga
      361 taataaatat ttatatagat acacaaaaga aaaggcgata tgaagctaaa cttaatgact
      421 tcatatcgtt ttttatttga aactcctttg tatttagagt gtgattttta atattgaggt
      481 tttatgtgat taagtcacag ataaaaagta agtatcttga tataattctt atagattaat
      541 aatataggga ggttattatg tctaataaaa aaaagaagta tgcaaatatt ttagcaaatg
      601 agtgcgttgc ttgtggtagc tgtataaaag catgtccaag aagtgctatc tcagttccat
      661 gtggcattag tgcaaaaatt aatagagatt tatgtgttgg atgtgggatt tgtgaaaaga
      721 tatgtccagc atctgtaatt gagattataa caatattaaa agaggaagaa ggtaagtgtc
      781 atgaataata aaaaacattg gtatgactat ttatggattt tttcttcaat atatttgata
      841 ttgggatttg taaatattat tttcgcttgg attgggctaa tttgtttctt tgttccacta
      901 gctatttcta ttgttaaggg aaataaagct tactgtaata agtattgtgg aagagggcaa
      961 ctgtttaatt tgcttggaaa taaattgaag ctgtcaagaa atagagatat tcctaaattt
     1021 attagaagta agtggtttag atatgggttt ttgacatttt ttatggtaat gtttgctaat
     1081 atgctttttt caacttattt agtgtttgaa ggaagtagaa attttaaaga agtagtgact
     1141 ttgttgtgga ctataaaaat gccttggcaa tgggcgtatc atggaacttt agtttcacct
     1201 tgggtggtac agtttgcatt tggtttttat agtgttatgc ttacatcaac tgttcttggt
     1261 ttgattacta tgatattgtt taaacctcgt tcatggtgtg tttattgccc tatgggtact
     1321 atgactcaga tgatatgtaa ggcaaagagt aagtctgtat agaaaggcct tatgtgatat
     1381 ttacagtttt gttcaagtag agatgagtag taggtttgaa tatatatgac aaatatatga
     1441 gtataggggc atagatttag tttgtgtaag gcaaactgat ttatgcttct tttttatgtc
     1501 ttagattatt ggctaagtag aaacaatgaa tttagatatt aagaattatt cagttatttg
     1561 aagaaaatag aataataatt aacattatat gaatggatat tcattacata taagcagtta
     1621 atagcaagat ttatttaata tgaaataggt caagtattta aactaaataa ggtcatgaca
     1681 attaatataa gtatagatat aaaaagatta gaagttataa cttataaaag gtattctata
     1741 taagtagtat gtggaaaata gtcaaattaa cttattagaa acaaaaaata tacataaaaa
     1801 ataaatgtaa atatcaaata agatactata taaaaataga tataagtatc ttttttatta
     1861 ttgaaattat gaaaatatat tttattttac acaaaaaaga gtgcctcatg aactaaaaag
     1921 ttcattttga gacacacttt tacagtgtca aagaacgaag tttatcctta tccaatactt
     1981 ttataccacc acgagataaa gacacaatac cttctctagc aaaatatttc aacattctcg
     2041 atacaacttc tctggcacta cccatatact tagcaatttg ttcatgagtc atatttatag
     2101 tgttactgcc atttttagct atctcatcaa ttaaaaaaga tgccaaacgc ttatcaaaac
     2161 tcataaaaag aatttgttgc atagcccaca taacatcaga aaaacgttcg gtagctaatt
     2221 tatatgaaaa taactctgca tgaatatttt ctgtagataa ttttgcaaaa acagaggaac
     2281 taatctgtat aatatcgcag tccgtttcag catctacata gacatcaaaa gttattgttt
     2341 ttagaataca ggaagctgat aaaatacata catctccatc atcaagtcgg aacagtgtaa
     2401 cctctcgacc ttcatctgaa agtatgtatg tacgcactgt tccagatttt ataatcaaaa
     2461 taccaataca gtcactatca ccacaatgta ctggattccc ttttttgtaa tgcgaggtat
     2521 ttgctgaaga aattaataat tctttttgta agtcagtaag tttatcccaa aaaggcaagc
     2581 tatgtgtaag tatatcaatt tcattttgtg aaatcataca atatctcctt tcttaaattg
     2641 ttataatccc atgatacaag tgaatagata aattgacaag tagtttgtta agaggattat
     2701 ttagtcagat agtaatagta tgatttatgt agaaaaaata tgataatata tattcttgct
     2761 attaatgaga aagtaatatg ttttgatgat agtaggtttg tataactaat gcataccgtt
     2821 tatttttaaa agattaagtt ccttatgtaa ccttatagac ataaaaccag caattatagc
     2881 agccatcaaa tcagctattg gttgtgcata taagatacca tttaacccat agaatacagg
     2941 aataatcaaa attataggga taaaacagac accttgtcta cacataccta aaaatccaca
     3001 ttcctttgct tttcctagtg cgaggaataa tgtagaatac actgtataga atccaaatag
     3061 cataaatgaa aacccatttg ccctaagagc tttactacca ttttctatta ataaaatgtc
     3121 accacttgta aattgtgaaa ttatagattt tgggaagagt atcaatataa gaccaagcac
     3181 tacacaaaat actgtagtcc ataatatgga agtttttatt gctatatgta atctatcgta
     3241 gttctttgaa ccaaaattat aaccagcaat tggttggaaa ccttttataa aaccaaatac
     3301 aacaagacta cctaaagatg ttattcgtgt tacagcaccc attccagcta tcacagaatc
     3361 accatacttt gtagcctcca tattagttaa tgtaatggca agactagtca ataattgaaa
     3421 tattaaggta ggtatgccaa tttttaggat ttctgaaaaa atttcttttg aaaaacagca
     3481 ttcgttaatt ctaaaactaa atatactttt cttgcttaaa atatatccta cataaacaag
     3541 tgttgaagca atctgtgaga tagctgttgc aatagaagca ccccttaccc ccagaccaaa
     3601 agtataaatg aatatcgggt ctaaaatcat attaagcaaa gcacctaata acatagcaca
     3661 catagttgtt ttagcagctc cttcacttgt gacaatatta gtcatagtga cattgaatat
     3721 attaaaaata gatgacacga cataaatgct agcataagat attgcgtaag gcataatact
     3781 ttttgtagct cctaacaatt ttaaaattgg attgagaaaa atcataatca caataataac
     3841 taatgtaccc actataatac tactgtaaat agatgtactt accacttttc ctgctttttc
     3901 attatcacca tggcctaaaa gtctggcaat atatgaagca gctccatttc caaacaataa
     3961 tcctaatccc acaacaagct gacctaatgg aaatgcaacg gatatagctc ccatttgact
     4021 tgttccaagt ccacccacaa agtatgcatc aaccaaattg tataatgcag ttatcatcat
     4081 acctatcatg gtaggtaatc ccatagataa caatgctttt gagattgggg cagagcctaa
     4141 taattcaatt cttttgtcac tttctttcat acttaacact cctcttctat tgacattaag
     4201 tctaaattat aatactataa attatagtaa gtatggaaaa tagtactata atttatagta
     4261 tttgtcaata gtaagaaaac taaaggagat gatttttatg gctacaatag atttaattgt
     4321 attgggaata gtaaagcaac aacccaagag tgcttatgat attcaaaagg cagtggaata
     4381 caggaatata tcacgttggg ttaaaataag tacaccatca atttataaaa aagtaattca
     4441 attagaacaa aagggatata ttgaaagtaa aactgtgaag aatggaaaaa tgccagaaaa
     4501 agcagtttat tcattaactg aattaggtga gacacatttt ttagatttaa tgtttgatac
     4561 atctagcaaa ccaataaata ttcttttaga ttttaatgct gttatagtaa atttaaatag
     4621 ccttacacca gaaaaacaga aattatgtct caataatatt gaagagaatg ttcaagagtt
     4681 aaagaaaact attgaggaaa atataagtat aaagacagaa gtaccagaga cagcaaaagc
     4741 agtattggaa caacagttgg tgctagcaca agcattggaa acctggataa attctttaaa
     4801 agataatatg ggaaattaga attagagaag ctaacttaat aatcattaaa caaaaaccag
     4861 catatttaat aatgagatat tagaaaaaat aaaacaaatg atataaactg aaaagtatat
     4921 ttaaaataaa gctcacttaa ttgagggaga atgttaaact ttt
//
LOCUS       DAGSRM010000154         4939 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.160, whole genome shotgun sequence.
ACCESSION   DAGSRM010000154 DAGSRM010000000
VERSION     DAGSRM010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4939
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.160"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(75..869)
                     /locus_tag="MJE33_003508"
     CDS             complement(75..869)
                     /locus_tag="MJE33_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9649414.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIVGIVMNILPVFILKIFGEAS
                     KTNLSTIVNPILTYITILLFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEIITGYSIELSVLLKRDMIWTSISALIDPSTSSMPLTYLNYF
                     VQICINEICIGFIGVLLGAFTYRVRKVTSITILIGLPILMIIYIVNFATKNMHKMLLY
                     LEKIIYSFQNPFILFGTKVGIIVICIIFIILLLRKAPIKEYANDLL"
     gene            complement(870..1745)
                     /locus_tag="MJE33_003509"
     CDS             complement(870..1745)
                     /locus_tag="MJE33_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9649415.1"
                     /translation="MSSSIKIENLSHSFKKQNIFNNMSLNFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSPDFKVGQIFEFSSS
                     FYKSYDKGLEEELCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILNEGKIIIDEEIDVIK
                     QKAHYITGNKEELEKLECIKNIKPKKAFGNAVAYFYYGDFSENDEKILKNSNIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(1749..2117)
                     /locus_tag="MJE33_003510"
     CDS             complement(1749..2117)
                     /locus_tag="MJE33_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBY9649416.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFVKNLLQEA
                     NKLEINREELVDIIINFKED"
     gene            complement(2300..2959)
                     /gene="deoC"
                     /locus_tag="MJE33_003511"
     CDS             complement(2300..2959)
                     /gene="deoC"
                     /locus_tag="MJE33_003511"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBY9649417.1"
                     /translation="MKHILKTVDHTILKATTTWEDIKILCDEAVDMSVASVCIPPSYV
                     KRASEYLKGKIKICTVIGFPLGYQTTATKVFEAKDAIENGADEVDMVVNISDIKNKDY
                     DNIGKEIKEIKKAIGDKVLKVIIETCYLDEDEKIKMCEIVTMSGSDFIKTSTGMGTGG
                     ATLEDIKLMKEHVGKNVKIKAAGGVKSISDAEKFIEAGAERLGTSSICKILKNEDTTD
                     Y"
     gene            complement(2995..4182)
                     /gene="murJ"
                     /locus_tag="MJE33_003512"
     CDS             complement(2995..4182)
                     /gene="murJ"
                     /locus_tag="MJE33_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBY9649418.1"
                     /translation="MKQEPCDFSHGRFRTGIIFIGITSVMSAFLQIKENFIVVGFGSI
                     PYNIVIIISIMLSTVFGPYILPIGAVVAMVVQLLFYMFFVKKTNYKYLYYLNFKDDSL
                     IKLLALLSPVFIGVAVNQVNSLVDTTLASTLVKGSIPALTYADRLNGFVTGTFTASIV
                     SVMYPMLSKLSAENNQKKFTSSVKSSINMIIISMIPISVASIFFATPVVRIIFERGAF
                     DARATQMTATALIFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIISVGVNIVLDLV
                     LIKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIKATLKSVVASLIMGVL
                     SYFTYKFIFGILGVGTFNEFVSLAISVIVGGGIYTLLMTIFKVEEVDMILNIAKRKLH
                     LKK"
     gene            complement(4259..>4939)
                     /locus_tag="MJE33_003513"
     CDS             complement(4259..>4939)
                     /locus_tag="MJE33_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBY9649419.1"
                     /translation="RILNATISKEPSGRYYVSLCCIDVDIEVFENTNNHIGLDLGIKE
                     FCISSCGEFIENPKYLKKSLNKLAKLQRELSRKTIGSLNRNKARLKVARLQEHIANQR
                     NDFLQKLSTKLIKENDIICIEDLQVKNMIRNRKLSRLISDVSWSEFIRQLEYKANWYG
                     RQIVKVGKFFASSQICNKCGYKNEEVKNLNIREWICPSCNETHDRDINASINILKEGL
                     RLITIQNK"
BASE COUNT         1639 a          836 c          491 g         1973 t
ORIGIN      
        1 catttattaa aactaataaa cctgctttaa aatacaaatt tacaaatttg atttttaagc
       61 aggttttatt ttagttataa taagtcattt gcatactctt taattggtgc ttttctaagt
      121 aataaaatta taaatattat gcaaataact ataattccta cctttgttcc aaataaaata
      181 aaaggattct gaaatgaata aataattttt tctaaataca atagcatttt atgcatattt
      241 ttagttgcaa aattaactat ataaattatc attaatatcg gtaatcctat caatatagta
      301 atactagtaa cttttctaac tctatatgta aaagctccaa gtaacactcc tataaaacct
      361 atacatattt catttataca tatttgtaca aaataattta aataagttag tggcatactt
      421 gatgttgaag gatctatcag tgctgatata gatgtccaaa tcatatctct ctttagtaaa
      481 actgatagtt ctatactgta acctgttata atttctataa ataacttcga taaaaaaatc
      541 aaaactatac ctaaaataga aatcacaaag cttaaaataa ttaaatctat gacaatagct
      601 tttatacaac tttttctatc tgctcttata cttaaagctc ctgaaaagtc tttatttgct
      661 atatttactc catacacaaa tagcagtata gttatatatg ttaaaatagg attcacaatc
      721 gtggataaat tagttttaga tgcttctcca aaaatcttga gaataaacac tggcaaaata
      781 ttcataacaa ttcccacaat cataaatata attatataca atttagtatt tttattaaaa
      841 aattttatat atggctttaa ttcattcaat tacaattcct ccttcttagt catgttaaca
      901 aacatatctt gtagccctat gtatcctaca tcaatatttg aatttttaag tattttttca
      961 tcattttcac taaaatcacc atagtaaaaa taagcgactg catttccaaa tgccttttta
     1021 ggttttatat tttttataca ttctaatttt tctaactctt ctttatttcc agtaatataa
     1081 tgagcttttt gttttatgac atcaatttct tcatctataa taatttttcc ctcatttaat
     1141 attattacat gctccagtaa atcatctatt tcatcaatca aatgagttga tattattatt
     1201 gtccttggat tttttatata actatctaaa attatgttat aaaactcttg tctatttact
     1261 gcatccagac caattgttgg ctcatcaaat atggtgatag ctgaattaga acaaatacct
     1321 attatatttg aaagtatagt tttcatacct cttgacagtt gtttatactt ttttttagta
     1381 tttaaatcaa aatatttgca taattcttct tctaagcctt tatcataact tttataaaaa
     1441 ctagaagaaa attcaaatat ctgacctact ttaaaatctg gactataaaa ttctttttcc
     1501 ctgacgatac aaacttcttc caaaactttt atatcttctt ttgggttctt tcctaatatt
     1561 tctatctcac cttcattttg aattaactga tttaccatta tatttaataa tgttgttttt
     1621 ccagctccat tcttacctaa taaaccatat atcttatttt tttcaaagtt tagagacata
     1681 ttattaaaaa tattttgctt cttaaaactg tgagataaat tttctatctt aatagaacta
     1741 ctcatatatt aatcctcctt gaaattaatt ataatatcaa ccaattcttc tctatttatt
     1801 tctaatttgt tagcttcttg aagtaggttt tttacaaaat tatgtttaaa attttctttt
     1861 ctagcttctt ttataatagt ttttgcacca tctgatacaa acatccctat ccccctcttc
     1921 ttatataaaa tatttttatc aaccaatata tttatacctt ttaatactgt agctggatta
     1981 attttataaa gctttgaaag ctctgttgtt gatggaacct gttcttcttc ttttattcca
     2041 tttgatagta tctcatcttc aattgctcgt gctatctgaa taaatatagg ttcttcatta
     2101 tttaatacta atttcatagg ttcctccttt cttcatcagt tagttactca actaattaac
     2161 tatataactt ataatatctt gaaaaatatt ttctgtcaat actaaataaa aaacagaggg
     2221 aaaattccct ctgttttttg ttctttatat ttataattaa attttgattt ttgtaatcct
     2281 atcccataag ttaaaaattt taataatctg ttgtatcttc attttttaat attttacata
     2341 ttgagctagt tcctaacctt tctgctcctg cctctataaa tttttctgca tcagaaattg
     2401 atttaactcc ccctgcagct tttattttta cattttttcc aacatgctct ttcataagct
     2461 ttatatcttc taaagtagct ccacctgttc ccattccagt agatgttttt ataaaatctg
     2521 aacctgacat tgttactatt tcacacattt ttattttttc atcttcatct aaataacatg
     2581 tctctataat gacttttaat actttatctc ctatagcttt ttttatttct tttatttctt
     2641 ttcctatatt atcataatct ttatttttta tatctgaaat gtttacaacc atatcaactt
     2701 catctgctcc attttcaata gcatcttttg cttcaaatac ttttgtagca gtagtttgat
     2761 atcccaaagg gaaccctatc actgtacata tctttatttt accttttaga tattctgatg
     2821 ctcttttcac atatgatggt ggtatgcaaa ctgatgctac actcatatct acagcttcat
     2881 cacataaaat tttaatatct tcccatgtag ttgtagcttt taaaatagta tggtctactg
     2941 tttttaatat atgtttcata aaatcctcct aaatttataa ttaatataat tagattactt
     3001 ttttaaatgc aattttctct ttgcaatatt cagtatcata tcaacctctt caactttaaa
     3061 aatagtcatt agtagtgtat aaattccacc tccaactata actgaaatag ctagtgatac
     3121 aaactcatta aatgttccta ctcccaaaat tccaaatata aatttatacg taaagtatga
     3181 taaaacaccc ataattaaag atgcaactac cgatttcaaa gttgctttta ttattttatc
     3241 ttgtccaaaa tatccaactt ttctctttag gttcaagaag agtaacaata tacatgcaat
     3301 tgatgatgaa ctagttgcaa gagcaagacc accatgagcc attggtttta ttaaaactaa
     3361 gtccaatact atatttaccc ctacagatat aatcccattt accataggtg tttttgtgtc
     3421 ttgtaatgaa taaaaaacct ttcccaaaat atctcttaag ccaaatgcag tcataccaac
     3481 tgcataaaaa attaaggcag ttgctgtcat ttgggttgcc ctcgcatcaa aagcacctct
     3541 ctcaaaaatg attctaacaa caggagttgc aaaaaaaata gaagcaactg aaataggaat
     3601 catagaaatt attatcatat ttatactgga tttaactgaa ctagtaaact tcttttgatt
     3661 attttctgca cttaacttag ataacatagg atacataaca gatactattg aagctgtaaa
     3721 tgtccctgta acaaaaccat ttaatctatc tgcataagta agtgctggaa tgcttccttt
     3781 aactaatgtt gaggctaaag ttgtatctac caatgagttc acttgattaa cagcaacacc
     3841 tataaatact ggtgaaagta atgctagtaa tttaataagg ctatcatctt taaagtttaa
     3901 gtagtataag tatttgtagt tcgttttttt aacaaaaaac atgtaaaaga gcagttgaac
     3961 taccatagca accacagcac caattggaag tatgtatggt ccaaatacgg tacttaacat
     4021 tattgaaata attattacta tattataggg tatacttcca aatccaacta caataaagtt
     4081 ctctttaatt tgtaaaaatg ctgacataac actcgtaata cctatgaata ttattcctgt
     4141 tctgaacctc ccatgactaa agtcacaggg ttcctgcttc atagaatttt gcatactaaa
     4201 atatgtctta ctaattctcc acaggctatc cccgtagttc ctacggttct tacatacgtt
     4261 atttattttg aattgttatt agtcttaatc cttcttttaa tatatttata cttgcattta
     4321 tatctctatc gtgagtttca ttacaactag gacaaatcca ttctcttata tttaagttct
     4381 taacttcctc gtttttatac ccacatttat tgcatatttg tgaacttgca aagaatttac
     4441 ctacctttac aatttgtcta ccataccaat tagccttgta ttctaattga cgaataaact
     4501 ctgaccatga tacatcagaa attaagcgag atagtttacg atttctaatc atattcttta
     4561 cttgtaaatc ttctatgcaa ataatatcgt tctcttttat taatttagta gatagttttt
     4621 gtagaaaatc atttctttga ttagctatat gttcttgaag tcttgcaact tttaatcttg
     4681 ctttatttct atttaaacta ccgattgttt ttctcgataa ttctctttgt aatttagcaa
     4741 gtttgtttaa tgacttctta agatacttag gattttctat aaattctcca caacttgaga
     4801 tacaaaattc tttaattccc aaatctaaac ctatatgatt attagtattt tcaaatactt
     4861 caatatctac atcaatacag cataatgata cataatatct accacttggt tctttagata
     4921 tagtagcatt aagtattct
//
LOCUS       DAGSRM010000155         4864 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.161, whole genome shotgun sequence.
ACCESSION   DAGSRM010000155 DAGSRM010000000
VERSION     DAGSRM010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4864
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.161"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            58..1395
                     /locus_tag="MJE33_003514"
     CDS             58..1395
                     /locus_tag="MJE33_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBY9649420.1"
                     /translation="MGTYSLISTSINFILILINIFIAFFLYTLTSYFIDFRNHWIFKI
                     ILILGYSIISQMIIWLSDPVNILFTLFGYFILLIICSKSKLIPIISVVMILYPIIIGL
                     NFLFINNPIYEELIHSKEYILILSIVQIISALIKLLFWFLIHIFLKNRLVNIKQYLSN
                     KIWIFIDMVCISSFLSILIAIILPPTNLIPMGSNKNIILGNLPYGTYLIILSAILANI
                     GVILLLQPLIENVKMKIEKQANHLKEKYYNLLENQQIQIRKMRHDMNNHFQMIESYLE
                     IEDIAGAKKYFNQLKIGIGTLSGKQFCKNQALNSILNLRHTSLEENGVDVHFNIDIDN
                     HLGIEPIDLCTIFSNSLDNAIEASLKIPDLSNRKVVLKARCEKEYFSYLLTNNKVNPI
                     NRKQELFISDKKDSNSHGYGIENIREIVNKYNGKLDISYTESEFSLFLYIRLF"
     gene            complement(1731..2300)
                     /locus_tag="MJE33_003515"
     CDS             complement(1731..2300)
                     /locus_tag="MJE33_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBY9649421.1"
                     /translation="MKKKLEDMTLEELWNLFPIFLTTHKECWKNWFKEEQYRLTGILP
                     MQKIVRISHIGSTSVSTIYAKPIVDILIEIIPDYNMKDIKDLIESNGYICMSESSSRL
                     SLNKGYTVDGFEERVFHLHIRYVGDNDELFFRDYLIDHPDVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKVLYSNRYN"
     gene            2703..3275
                     /locus_tag="MJE33_003516"
     CDS             2703..3275
                     /locus_tag="MJE33_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HBY9649422.1"
                     /translation="MEYIINPCSGIKIKITRGQKIKIVDIEGGQVVDFFAEMKDDSTE
                     FISPGVTIDCNESLRLQVGNLIYSNKYRPMFKILYDDVREHDLLHPCCRPEMYDFFYH
                     NGENHPNCFDNINRTLGTSHQIIHPINFFMYTKVNTDGSIEVCEPLSKASDKIILEAL
                     ENMTLGIAACSVSESSCNSRKCTSIKILVD"
     gene            complement(3954..4856)
                     /locus_tag="MJE33_003517"
     CDS             complement(3954..4856)
                     /locus_tag="MJE33_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBY9649423.1"
                     /translation="MLPQDYYGLIVILGVILGVMGAFYNWFTLYVQSLYKKIPKIGTF
                     GKLLIPFIMAGILGLVMPDVLGSGHNLVEELTSHKYIFGMVVLIFVIRFIFSAVSFGS
                     GAPGGIFFPLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPLTG
                     IILIFEMTGSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKERGE
                     KILDQFSVMFGSPVANKAIQDVQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVTMT
                     DERDSGAVYDKMENLCKEHTAHIF"
BASE COUNT         1758 a          805 c          573 g         1728 t
ORIGIN      
        1 gatttaattt tttaaaactt agtttccaga atcttaatta atagaaaggt atattaaatg
       61 ggcacttatt ctttaatctc tacaagcata aattttattc taatattaat caatattttc
      121 atagcctttt ttctttatac gttgacttcc tattttatag actttaggaa tcattggatt
      181 ttcaaaatca tattgatttt gggctactct attattagtc aaatgataat atggctttcc
      241 gaccccgtaa atattttgtt tacactattc ggatatttta tactacttat aatatgtagc
      301 aaaagcaaat taattcctat aatatcagtt gtcatgatac tttatcctat tataattggg
      361 cttaattttc tttttataaa caaccctata tatgaggaac ttatacactc aaaagagtat
      421 attttaatac tttcaatagt ccagataatt tctgctctaa ttaaattatt attttggttt
      481 ttaatacata ttttcctaaa gaatagattg gtcaatataa aacaatactt atcaaataaa
      541 atatggattt ttattgatat ggtatgtata tcttcatttt tatcaatatt aattgctatt
      601 attctgccac ctactaattt aatccctatg ggtagtaata agaatattat cttaggcaac
      661 ttaccttatg gcacttatct aatcatcttg tcagcaattc tggcaaatat cggtgtgata
      721 cttcttttac agccattgat tgaaaatgta aagatgaaga tagaaaaaca agcaaatcac
      781 ctaaaagaaa aatactataa cttactagaa aatcaacaaa ttcaaatacg caaaatgcga
      841 catgatatga ataatcactt tcaaatgatt gaatcgtatt tagaaattga agatattgct
      901 ggagctaaaa agtattttaa tcagttaaaa ataggtatag gtactcttag tggcaaacaa
      961 ttttgcaaaa accaagctct taactcaatt ttaaatcttc gtcatacaag tttggaagaa
     1021 aatggtgttg atgtgcactt taatatagat atagacaatc acttgggtat agaacctata
     1081 gatttatgta ctattttttc aaattcttta gataacgcta tagaagcatc tttgaaaatt
     1141 cctgatttgt caaatagaaa agtggtatta aaggcacgtt gtgagaagga gtattttagc
     1201 tatttattga caaacaacaa agtaaatcct atcaatagaa aacaagaact atttatttca
     1261 gataaaaaag atagtaattc tcacggatat ggtattgaga atattagaga aattgttaat
     1321 aagtacaatg gaaaattgga tatttcttat actgagagtg aattttcttt atttctttat
     1381 attagattat tttagtctaa aattagatta tcttatataa tacaaattag attgtttgat
     1441 actatgtaaa attataataa cttataatta aatatttaca catatattca ttaacttaaa
     1501 atttaatcaa atgtgatatt ttatttacct tagtaaaaat gtgtgtgtct caaaataaac
     1561 tttttagttg atgaggcact cttttttaca tgaaatcaaa tatattttta tcctttcaac
     1621 aatgaaaaag ggatttatct ctccattttg aaacaagccc tttttctatt aattactttc
     1681 ttaaaaaata taatacgata tataagataa atatctaatt gtacctattt ctaattatat
     1741 ctattagaat acaacacctt tgccttttca gtaaactccc taacaaaatc tagtttacta
     1801 tctgtataag catcacgatt atgctcatac ttcttccaaa ggtccaactt aagtttttca
     1861 tactcctttg caacatctgg gtggtcaatt aagtaatctc taaaaaacaa ctcatcatta
     1921 tcacctacat accttatatg caaatgaaaa actcgttctt caaacccatc tactgtatac
     1981 cctttattaa gtgaaagcct actactgctc tcagacatac aaatatatcc attcgactca
     2041 atcaaatctt taatatcttt catattataa tcaggaatta tctcaatcag tatgtcaaca
     2101 atgggcttgg cataaatcgt actcacagaa gtacttccaa tatgacttat tctaacaatt
     2161 ttttgcattg gcaatattcc agttagtcta tattgttctt ctttaaacca atttttccaa
     2221 cattctttgt gagtagtcaa aaatatagga aacaagttcc ataactcctc taaagtcata
     2281 tcttccaact tctttttcat tagttacctc cctttaaatt tgtactttat attttttctt
     2341 tttcaaattt acttagttaa tatttaactt tattttttat caaatcattt ttctaattat
     2401 gtaaattttt ctaaatttag tttttaatgt attataacag atttttaccc ttcttacact
     2461 tttgtatcta tttaaatatt agaacatttt ttcttacttc taaatacagt aagattgctt
     2521 aaataccaaa taggagcaaa aaaactctgt aatcttaaaa aaactacaac atctttatga
     2581 gaattaaaaa atgatagact atgttatcaa cattaagaaa cagatagaaa agaaacagag
     2641 tatgtgctaa aatgggaaca tagaacagaa tgacaaattg gaatttatgg aggacattaa
     2701 atatggagta tataatcaat ccttgtagtg gtataaaaat taaaataact agagggcaga
     2761 aaattaaaat tgtggatatt gaaggaggac aggtcgttga tttttttgca gaaatgaaag
     2821 atgattcaac tgaatttata tctccaggtg ttacaattga ttgtaatgag tcattaagat
     2881 tacaagttgg gaatttaata tattctaata agtatagacc tatgtttaaa attttatatg
     2941 atgatgtaag ggaacatgac ttgctacatc catgttgtag acctgagatg tacgatttct
     3001 tttatcacaa tggagaaaat catccaaact gttttgataa tattaatcgc acacttggaa
     3061 cttcccatca aattattcac cccattaatt tttttatgta cacaaaagtc aatacagatg
     3121 ggagtattga agtatgtgaa cctttatcaa aagccagtga caaaattatt cttgaagcat
     3181 tggaaaacat gactttaggg attgctgcat gcagtgtttc agaaagttct tgtaatagca
     3241 gaaaatgtac ttctattaag attttggttg attgacaaat ttcatttatc gaaaaaatat
     3301 caaaattaaa aaatgacgtc aaaggcgcaa aagggtaacc acttgcgctc tcaattcatt
     3361 atggtttgtt acgtatttat gggtaaaaat cccttgattc tatgatgttt ttcagacaca
     3421 attacaaatg agtaaggatt ttatactcat actctaaaaa atagcatttt attgcgtaac
     3481 agaacgaaaa aacagtgtgt acctttttct tgcaatacac actgtattca tagccactct
     3541 agctgttaac tgttaattat ctcataatac ccttactggt attcatctaa tcttaacccc
     3601 ttttaattat tttcatttat aaataaattc tattcttccc aaaatatcgt aattaaaata
     3661 tattaattaa aatatcccaa taagaatatc tgaaaaatct tctacctatt ttttcaaacc
     3721 ttttattccc attcctcata caatagtgca acttttagca cagtataaat ataccattag
     3781 tccatataat ctcattaaca aatatataca attattttct tcaaactctt ttatatctaa
     3841 gatatcacat tttcattttc ttatattaat tccttaactc tctttcaaat taccaatttt
     3901 ttaatcaaat cttcattaaa tattctatca ttattaaaca ttcatcatta tccctaaaaa
     3961 atatgtgccg tatgttcttt acacaaattt tccatcttat catacacagc accactatct
     4021 ctctcatctg tcatagtcac aattacatca cttggtctaa gaattgtctt acctctagga
     4081 ataatttctt ctccacctct ttgaattgct accaaaagac aattttcagg ccactgtaca
     4141 tcctgaattg ccttgttcgc aacaggagag ccaaacataa cagagaactg gtctagtatc
     4201 ttttcgcccc tctctttatg tacaggttct ccattctttt caagtaacct ctcaagaaga
     4261 ctttcataaa taggtttaga cttcataagt gtagccacaa tatatgccac aattgaaatt
     4321 actgatagtg aaagcatttg actaacagaa cctgtcattt caaaaattaa tatgatacca
     4381 gtcaaagggg cacgcacaat agctgtaaaa tatcctgcca tagccaaaag cacaaagtta
     4441 tttacataat cgggattcat tccaaaaact ttaacaccta ccataccaaa tattccacct
     4501 ataaatgctc ctaaaacaag caatggaaaa aatattcccc ctggtgcacc tgacccaaaa
     4561 ctaacagccg aaaatataaa acgtataaca aaaatcaaaa caaccattcc aaaaatatat
     4621 ttatgacttg taagttcttc aactaaattg tgtccactcc ccagaacatc aggcataaca
     4681 agcccaagaa taccagccat tataaatgga atcaacagtt ttccaaacgt accaatttta
     4741 ggaatcttct tgtataaaga ctgcacatat agagtaaacc agttgtaaaa tgcccccata
     4801 actccaagaa taacacctaa aatcactatt aatccataat aatcttgtgg caacacattt
     4861 ccaa
//
LOCUS       DAGSRM010000156         4673 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.162, whole genome shotgun sequence.
ACCESSION   DAGSRM010000156 DAGSRM010000000
VERSION     DAGSRM010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4673
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.162"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(19..165)
                     /locus_tag="MJE33_003518"
     CDS             complement(19..165)
                     /locus_tag="MJE33_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649424.1"
                     /translation="MDMDLLFKIVFAIVAIGVSVKIIKTVASLAFKIALILLIILFVY
                     RMFL"
     gene            complement(304..1230)
                     /locus_tag="MJE33_003519"
     CDS             complement(304..1230)
                     /locus_tag="MJE33_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBY9649425.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDDHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIAFLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTSRGFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLVLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIPFISILLLS"
     gene            1420..3291
                     /locus_tag="MJE33_003520"
     CDS             1420..3291
                     /locus_tag="MJE33_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBY9649426.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKMESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLGYLQAQNNLGVCHFKMKDFVRCEKWLKTAADKNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHTLS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNVSCQIKLGNIYEDLNNIEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     SKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYISLANEEYDNCEEIVEGFP
                     NLNIDFDEVLIPPFKLQEEVIDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDS"
     gene            complement(3383..4318)
                     /locus_tag="MJE33_003521"
     CDS             complement(3383..4318)
                     /locus_tag="MJE33_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBY9649427.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSNNPNGFWIILLITSIICIVITFFMSRNKLL"
BASE COUNT         1849 a          523 c          715 g         1586 t
ORIGIN      
        1 aaacttattt ttaaatgttt acaaaaacat tctatataca aataaaatta ttagcaatat
       61 caacgctatt ttaaaagcta aactagcaac agttttaatg atttttacag aaacacctat
      121 agcaactata gcaaatacta ttttaaagag taaatccata tccatattta agcctcctat
      181 tctataatac atatagatat tattgacaaa tatctaggaa aaaattccat caatatacta
      241 caataaaatg tttatatttg taatttattg aatatgttat tatataatac ataaaaaaat
      301 aaatcaagat aagagtaaaa tacttataaa aggtatagat ataactgaaa atagtgttgt
      361 aaaaaacaca cattgtgaag ctagtgttat attggagtca tattgattag ccattatagc
      421 agtatttgca gcagcaggca ttgaagaaat tactactggt atagctaaaa tcatcttatc
      481 atttatccat ccttttagaa taaaatacac aatcacagga agtaccaata atcttataaa
      541 agtgactata tataattttt tgttgacaaa acaatcgaga gctgaagaat tagcaagtaa
      601 gcttccaatg ataatcatgg atattggtgt agttatactt cccaacatct caagtggctc
      661 gtttataaat tggggtaacc taagtcctgt tacaaataaa aaaagtccaa taacaatagc
      721 tattgtagca gggcttataa gtgattttat tgaaaaacct ctacttgtac tgcctttaga
      781 taaaagataa attcctaaag tgaaagaaaa taagttaaaa ggcaaattga atattgcagt
      841 atagaatatt gcttcatgac ctaaaactgc ttcaattaca ggataaccca taaaagcaac
      901 atttgaaaaa acaactatat actggtatat tcccaaatct tttttatcat tacattttaa
      961 aatgtatttc aataaaaatg caattataat tgaaactata tacataatca aagatgtaaa
     1021 taataacaat agtatttttt gtatcatttt actatcaaag ttaatttgca tggagctaat
     1081 tatcattgat ggtaaaaaaa ctgtcattgt caaagttgaa agcttagatg tgcagtggtc
     1141 atctaataag ttgaattttc tgacaaaata tccaactaaa ataattataa ataaaactgc
     1201 aacttggata aaaatattgc tgaaattcat aaaatgtccc ccctatatat acattatata
     1261 ccaattacat aataaaatct aatgattata aaatttaaaa tttaatgtta caatatagtg
     1321 acaaaaggta aaaattcaat attatagcat taattgtaga ttataatgta taataatgtt
     1381 tgagagaata tattttataa aatgggtagg tagcgaataa tgaaaaatac aaaattttct
     1441 ataaaaaaaa taaaaaatat atctaatgac ccgaaagtcg tacatactaa gggtgccctc
     1501 cttgaaaaga tgggtaagat ggaaagcgca atagagttgt ataagagtgc tgcattggat
     1561 aattacgtaa aatctcaata tactttagga aatatatatg aaagtaaaaa acaatttaaa
     1621 gaagcagaaa agtggtattc aatggcatat aaaagtggaa gtgaagatgc tgcttttgat
     1681 ttaggtaata tgtactataa gttagatggc tatgaatatg ctatatattg gtatgaaaag
     1741 atagcaaatt taggttattt acaagcacaa aataatttag gtgtatgcca ttttaaaatg
     1801 aaagattttg taaggtgtga aaaatggtta aaaacagcag cagacaagaa tttaggtaaa
     1861 gcttgtttta atttaggggt attatatact tttttggaga aagatgatga agcatatgaa
     1921 tactataaaa aaggttctgt gcttttagat gatgatgcaa aatataattt agcaatatta
     1981 aatggaaaaa agaaagataa taaatcaaca gtaagtttat ataaacagtt gtacaagtca
     2041 ggtcatatga aaggttgctt taacttgggt atgattatgg aaataaatga taatttagaa
     2101 gatgcagaga agtattataa aaagagtgct gatagagatg atgtgaagtc tgagtatagg
     2161 cttgcatatg tatatgatag aaaagaagag ctgggagatg caatttatta ttatgagaaa
     2221 gcaatagaaa aaaatcatac actttctaag tatagacttg ctaatttata taatagagaa
     2281 aatgagatag agaaggcaaa aacttattat aaaatggctg ctgaagatga tataacagaa
     2341 gcaaaaaata atctggcagg tatatatttt gaagaaaaag attatgaaaa tgctattaaa
     2401 tattatgagg atgctatagc tgttggatgc aaaagttccc ttgaaaatct aggggattta
     2461 tattatcaga atcaagatat agaaaaagcg atttcgtatt attcgaggat tccaaataat
     2521 gtatcttgtc aaatcaaatt aggaaatatc tatgaagatt taaataatat tgaagaagca
     2581 ataagttggt ataagaaagc atctgaaaat ggagatacga ggtctagtta tagattgggg
     2641 tgtatatatg agagcttagg aaatacaaaa aatgctagaa agtattttga aatggcaagt
     2701 agcaaaaacc atatgaatgc tcgtatacat ctaggtagaa tttattttag agaaggtaaa
     2761 ttggaagagt ctaaaatgat gtttgataca cctgccaatg aaaataatgt gtatgctcaa
     2821 catatggtag gattaattta tgatatgttc tataaagatt atgtaaattc taaattttgg
     2881 tatgaaaagg ctagagcaca aggttgtgtt gaatcaatat ataatttagg tcaaatttat
     2941 ttaaaattaa atgatgacgc agaagctgag aaatattata aagaaggtat taaatatgga
     3001 agtaagaagt gtgagtatat gcttgcaggt ctttattata aaaagagcct agatatgtac
     3061 atttctttag caaatgaaga gtacgataat tgtgaagaga tagtagaagg ctttccaaat
     3121 ttaaatatag actttgatga agtattaatt cctccattta agttgcaaga agaggtaata
     3181 gatgaagaag agtatgtacc aatttatata ctaaatataa aagaaagttt agattcaatg
     3241 ctagaagggc ttgaaactga aatgattata gatgaagaac atgatagtta aaaagtatta
     3301 taagattata tagatttaaa tatataaaag ccattaggat aaatgtaaat caattaattc
     3361 taatggcttt tttattaata ttttataata gcttatttct tgacatgaaa aatgtaatca
     3421 ctatacatat tatagaggta ataagaagta ttatccaaaa tccgtttggg ttattagaaa
     3481 atggcatgtt gatgacattc ataccaaaga atcctgatat tattgtaggt attgagaaaa
     3541 ttagtgtaac tactgttaaa atttgcataa cattattctg attattactt ataattgcac
     3601 tgaaagcatc cataattcta ctcaatatat ctccatatat tttagccata tcaagagcct
     3661 gtctattttc aactataaca tcttctaata ggtcttcatc atctggatac ttctttatac
     3721 cagcagttct aaccattttg tttaaaacaa gttcattaga ttttagtgag gtagagaagt
     3781 ataccaaaga tttttctaat tccaataatt gaaccaattc tttgtttttc attgatttgt
     3841 aaatttctcg ctctattatt attgtcattt tatttatttt tcttaagtaa tgcaagtaat
     3901 aagctgcatt tttgtgaaga atttgaagca aaaagcgagt ctttttaaat gtaaaaaaat
     3961 ctttaatatg accaactata aagtcattta aaatcttagt ttgtgcatca caaactgtta
     4021 caatagcttc ttcagttagt ataataccta agggtattgt agtataatga gaagaattag
     4081 agtcactttc gtctactggt atatctataa gtataagagt atgattgtct tcaacatcta
     4141 ttcttgagcg ttcttcttca tctagtgcag attctatgct ttctacatct atatttaata
     4201 aattagaaat ttcgttgatt tcactatgat taggttcaac aagatttatc caacagccat
     4261 cttcaaaaaa actaagtttc tctaattttg aatctatagt tttgtaatat cttatcatgg
     4321 caacacatcc tttattcagt tttatataaa tctcatcatt taatagtatc tattaattaa
     4381 ctaatttata taactgaata tatgtattag ttttgatttt agatattatt aagtgatgaa
     4441 aatgtaaaaa attctctccg caacggacta gtatccattg cccatcactc tccttattta
     4501 ttattttata tatagctatt tatgaaatgc gtattttaag cactctttaa tataatacct
     4561 tttccaggta tataaatcaa gttttgattt gattaaataa tatttttacc ataatttaca
     4621 cataaagtat ataagttata tttaatttga gtaaagaata tatatattct gag
//
LOCUS       DAGSRM010000157         4634 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.163, whole genome shotgun sequence.
ACCESSION   DAGSRM010000157 DAGSRM010000000
VERSION     DAGSRM010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4634
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.163"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            231..1310
                     /locus_tag="MJE33_003522"
     CDS             231..1310
                     /locus_tag="MJE33_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBY9649428.1"
                     /translation="MPELLKNMYNRESLYEVAVAIQSVYNSFKVDEFIKSTMDETWNN
                     LELKARCRKISMSLGMYLPEDYKEALSILEKSVTGFYFAFFFPDFVEVYGQDDINWDL
                     SISALERNTEYWSSEFAVRAFIIKDEERMMAQMRKWSKHKSEHVRRLASEGCRPQLPW
                     GQAISKFKKDPTPVLPILEQLKTDTSTYVQKSVANNLNDISKTHPDLVISIAKDWYGK
                     NKSTNWIVKHGCRTLLKKGNRDVLALFGYDDTTSINIQDFTLETTSISIGEDLTFSFN
                     ILAKKATKTRLEYGIDYIKSNGKKSRKIFKISEVSLKENEKKSYMKKHSFADVSVRKH
                     YPGIHSIAIIINGIEKDKLDFELGI"
     gene            1634..2002
                     /locus_tag="MJE33_003523"
     CDS             1634..2002
                     /locus_tag="MJE33_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HBY9649429.1"
                     /translation="MDVYSFWNDTLAQDAVKIRKYFHENAYINWHDTNEHFTLEEFII
                     ANCEYPDKWNGKVERVLEFGNLVITVTHVYAINKPLSFHVVSFISLKDDKIISIDEYW
                     GEDDDVPQWRLDKHIGKPIK"
     gene            2119..2922
                     /locus_tag="MJE33_003524"
     CDS             2119..2922
                     /locus_tag="MJE33_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBY9649430.1"
                     /translation="MEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLFDFQ
                     IQYFSKNHNVLVWDAPAHGKSRPYLDFSYSNLVEELKVILDKQNMKKVVLIGQSAGGF
                     VAQSFIVKYPNMVEGLMMIGTCPYGTDYYSKSDIFWLKQTEWMLKLFPDKFLRNIISK
                     MCGATSEGRRSMLKMLEGYEKKELCHLMYLGFAGFIPEIQDLNIHCPVCLVVGDKDKT
                     GKVRKYNEQWHNKTGYPLHIIKNASHNVNVDKPNELNQIMDIFIKQLNI"
     gene            3307..4197
                     /locus_tag="MJE33_003525"
     CDS             3307..4197
                     /locus_tag="MJE33_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBY9649431.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKQAL
                     DLISENHFGVSIDTKSSLVVRDIPILQKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSVRFEAVKELNDSGIFCGILLTPMLPFLTDTKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRDISPKLVLKYQKTYGLNYVCTVQNKDSCEKLLREECARYGILTD
                     MKEIIKAYKRSESYTQIKFF"
BASE COUNT         1762 a          489 c          870 g         1513 t
ORIGIN      
        1 cagtaaagta taaaattcta ttactataat aaaagataga actagaagtc tttatgaaag
       61 tgggaaaagt gtttatataa ataaatttat atagtttata gtttaacagt aggaaatatc
      121 gtatgagtga gaatatcgta ttttacataa agatttagca atatgacaca ctgttgtcgt
      181 gttctgtagg atatgattaa taaatcagat atgaaaggag agtgagtaat atgccagaat
      241 tactaaaaaa tatgtataat cgtgagtcac tttatgaagt tgcagtagct atccaatcag
      301 tttacaactc atttaaggta gacgagttca taaaatctac aatggatgag acatggaata
      361 acttagaatt aaaagccaga tgtcgaaaga taagtatgag tttaggaatg tatttaccag
      421 aagactataa ggaagctctt tccattcttg aaaaatcagt tacaggtttt tattttgctt
      481 ttttctttcc agattttgtt gaagtttatg gtcaggacga cataaattgg gatttatcta
      541 taagtgcatt agaaagaaat actgagtatt ggtcttcaga atttgctgta agagcattta
      601 tcatcaagga tgaagagcgt atgatggcac agatgcgtaa atggtctaag cataagagcg
      661 aacatgtacg aagacttgca agtgaaggtt gtcgtcccca gttgccttgg ggacaagcaa
      721 tatccaaatt taaaaaagac cctacacctg tactgcctat attggagcaa cttaaaacag
      781 atacatcaac gtatgtacaa aagagtgttg ctaataatct aaatgatatc tcaaaaacac
      841 atccagattt agtcataagc attgcaaagg attggtatgg taaaaataaa tccactaact
      901 ggattgtaaa gcatggttgt agaactctgc taaaaaaagg aaatcgtgat gtattagctc
      961 tttttggata tgatgatact acttcaataa atattcagga ttttacttta gaaacaacgt
     1021 ctatttctat tggagaggat ttaacttttt catttaatat tttagcaaaa aaagcgacaa
     1081 aaacaaggct cgagtacgga attgattata ttaaatctaa tggaaaaaag agtaggaaga
     1141 tatttaaaat atcggaggtt tcattaaaag aaaatgagaa aaagtcatat atgaaaaaac
     1201 attcatttgc agatgtaagt gtaaggaaac attatccagg tatacactcg attgctatta
     1261 ttattaatgg tatagaaaaa gataaacttg attttgaatt aggtatataa ttttactaac
     1321 agaataatct atatcagata ctttgctaaa cttatataat atattttact aaatttatat
     1381 taggtagttt actacttgtt tactgaattt aaatacaaag aaatccaggt gttaatccct
     1441 gccgattaaa ggaatatgag cataacacaa aagtagaact tatcaattct gttacgggta
     1501 tagtagtaaa tccatagaat cacaattaga agatgaagtg taaaaaataa gttagtgttg
     1561 attttagttg ctagtgatat tgaatagtta tgcaataccc atataaatta gaatttgtag
     1621 aggagaaatg taaatggatg tatatagttt ttggaatgac actttggcac aagatgctgt
     1681 taagataaga aaatattttc atgaaaatgc ttatattaat tggcatgata caaatgagca
     1741 ttttacttta gaagaattta ttattgcaaa ttgtgagtat cctgataaat ggaatggtaa
     1801 agtagaaagg gtgctagaat ttggaaattt agtaattaca gtgacacatg tttatgcaat
     1861 aaataaacca ctatcatttc atgtcgtttc atttatcagc cttaaagatg ataagattat
     1921 ttctattgat gaatattggg gagaggatga tgatgtgcca cagtggagat tagataaaca
     1981 tattggaaag ccaataaaat agtgtttgtt ctgttttata actgaaattt tgataacatt
     2041 tgatattttg atttgtcatt ggtcaacata gtcgttagtg taataattta aaaaagtaga
     2101 atttggagag tgaacactat ggaagaaaaa tacatcttat ctgaccgagg gaaagtatat
     2161 tattggataa aagaaagtaa tagtgtgttt gcaaagaact taattttttt gccaggacta
     2221 acagcaaatc atcatctttt tgatttccaa atacagtact tttcaaaaaa tcataatgta
     2281 ttggtatggg acgctcctgc tcatggaaaa tcaagacctt acttagattt ctcatattct
     2341 aacttggtag aagaattgaa agtaatttta gataaacaaa atatgaaaaa ggttgtactt
     2401 atagggcaat cggcaggggg atttgtggca cagtccttta ttgtaaaata tccaaatatg
     2461 gtagaaggat taatgatgat tggaacttgt ccatatggaa ctgactatta tagtaagtca
     2521 gatattttct ggttaaaaca gactgaatgg atgttaaagt tattcccaga taaatttttg
     2581 cgaaacataa tatctaagat gtgtggagct acgtctgaag gcagaaggag tatgctgaaa
     2641 atgttagaag gctacgaaaa aaaagaactg tgtcatctca tgtatctggg atttgctggg
     2701 ttcataccag aaattcaaga cttaaatata cattgtccag tttgtttggt ggttggagat
     2761 aaagacaaga cgggtaaagt gagaaaatat aatgagcagt ggcacaataa gactgggtat
     2821 ccattgcaca ttataaaaaa tgcttcacat aatgtaaatg tcgataaacc taatgaacta
     2881 aatcagatta tggatatatt tattaagcaa ttaaatatat aaaaactaca tactcattag
     2941 gtaaattatg tagtatatgt gtttacagat ggttatagaa ataaatatgt aataaactac
     3001 ttaacactta aaaagtggat ttgtccagag cataatacaa agcatgatat ataaattttt
     3061 aagcgaaata catcaaaaga agtattagaa ttagtggaga taaaacagct attagagatt
     3121 ataaataaat agttttaaag gtactacgaa tatagaggaa ggtaaatcat acgttattca
     3181 aggattataa atgattagtt taaaagtact atcggatagg tctctgcata tttaaacctt
     3241 agtaaaggta gttaaagtag tagtttaaaa actaaaaaat tatataaagg aattaggtat
     3301 aagaaaatga ttgagagtat accagcaaaa caaattttac aaaaagtgaa atttgacaat
     3361 acaagatggt ttggaattga ctataatatg aatctctatc gtggttgttc tcatggatgt
     3421 atatattgtg atagccgtag cacaatatac aacatagaga attttgacaa agttagatat
     3481 aaagaaaatg tgatagaaat tttaagtaaa gaattgcgtt caaaaagaaa aaaaggtgta
     3541 gtaggcattg gagctatgtc tgacacatat aatccttttg aaaagcaact ttgtattaca
     3601 aagcaagcac tagatttgat ttcggaaaat cattttggag tgtctattga tactaaaagt
     3661 agtttggtgg ttagagatat accgattttg cagaaaatta aaaaaaataa ttctgctatt
     3721 gtaaagttga ctataaccac tgcaaatgat gaattatcaa agaaaataga accttatgta
     3781 aatccaagtt ctgtacgatt tgaggcagtg aaagaactta atgattctgg aattttttgt
     3841 ggtatattat taactcctat gcttccattt ttgactgata ctaaagatga aatacgagca
     3901 attgtagaaa aagcacacaa ggctaatgca aaatttattt attgcatgta tggtgtaaca
     3961 atgcgtagtg gtcagagaga gtttttttat gagcatttaa gagatatatc accaaaattg
     4021 gtacttaaat atcagaaaac atatggttta aactatgtat gtactgtaca aaataaagat
     4081 tcttgtgaga agttgttaag agaagaatgt gcacgttatg gtattttgac ggatatgaaa
     4141 gagattataa aagcgtacaa gaggtcggag tcatatactc aaataaaatt tttttaagat
     4201 aggctttgga tgagatataa tagtggtgat tgatgtgttg atattatagt tgattgttag
     4261 agaggatatt atagatgaat attagtattg atattctagg taattgctag agttagtatt
     4321 atagatgaat attaatattg atattctagg taattgctag agttagtatt atagatgaat
     4381 attagtattg atattctagg taattgctag agctaatatt atagataaat attagtattg
     4441 atattctagg taattgctag agctgatatt atagatgaat attagtattg atattctagg
     4501 taactgctag agctaatatt atagatgaat attaatattg atattctagg taattgctag
     4561 agctgatatt atagatgaat attaatattg atattctagg taattgctag agttagtatt
     4621 atagatgaat atta
//
LOCUS       DAGSRM010000158         4538 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.164, whole genome shotgun sequence.
ACCESSION   DAGSRM010000158 DAGSRM010000000
VERSION     DAGSRM010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4538
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.164"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            284..1759
                     /locus_tag="MJE33_003526"
     CDS             284..1759
                     /locus_tag="MJE33_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBY9649432.1"
                     /translation="MDSEFANAVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVSAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITLEKV
                     RTEIEKDCNIKALFFLNPTYYGVCADLESIIELCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            1846..2259
                     /gene="speD"
                     /locus_tag="MJE33_003527"
     CDS             1846..2259
                     /gene="speD"
                     /locus_tag="MJE33_003527"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HBY9649433.1"
                     /translation="MKFETLGRHILVEYYNCDENVLKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            2289..3140
                     /gene="speE"
                     /locus_tag="MJE33_003528"
     CDS             2289..3140
                     /gene="speE"
                     /locus_tag="MJE33_003528"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HBY9649434.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSCYPQIEKI
                     DMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            3130..4008
                     /gene="speB"
                     /locus_tag="MJE33_003529"
     CDS             3130..4008
                     /gene="speB"
                     /locus_tag="MJE33_003529"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HBY9649435.1"
                     /translation="MKNNFYHRNTFMSMNKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     VPFMIGGEHLVTLPAFKAVHEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFENIVNMLNGKNIYLTIDLDV
                     LDAAVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISDKIK"
     gene            complement(4188..4388)
                     /locus_tag="MJE33_003530"
     CDS             complement(4188..4388)
                     /locus_tag="MJE33_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBY9649436.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
BASE COUNT         1819 a          504 c          764 g         1451 t
ORIGIN      
        1 aaactcaaaa aagtaaaaaa accaaaataa taaaaaataa gaaagtttat aaatactaag
       61 ttttaaaaaa attaatataa acaacaaaaa attaaaaagt ttataaatat tgatatagct
      121 aatgtatatg tttttgagta acttcaaaaa aacacttgta aaaatgctga gatgttaata
      181 taataaatat catgagttta ataaaagtaa aaataaaaaa tagaaataat aaataataaa
      241 tattaaaaat atttgaatat agagaggaga tgaatttgaa atcatggata gcgagtttgc
      301 aaatgcagtt gaattaagct atgagtgtgc tccattatta gagtcgttaa aggaatactc
      361 agagaaagat atagcttgtt ttgatgtgcc tggacatgta aaaaatagag gtgtagctat
      421 attaaataaa tatctaggtg aaagtctcat gaaaatggat ataaattcat caccaactat
      481 ggataatgta tctgcgccaa atgggattat aaaaaatgca caagatttgc tagcacaagc
      541 atatatggct gatgaggcat tctttataac gaatggcaca actcaagcaa tacatgcaat
      601 gattttgagt gttataaaac caggtgaaaa agtattactt ccaagaaata tacataaatc
      661 agtaataaat gcgttaatac tttgtggagg aataccaata tttatacaac cagaatttga
      721 tgaaaaacta ggtataagtt taaatattac tcttgaaaaa gttagaacag agatagaaaa
      781 agattgtaat ataaaagcat tatttttcct taatccaact tactatggag tttgtgcaga
      841 ccttgagagt attatagaac tatgccataa aaataatgtt ttagtgttag ttgatgaagc
      901 tcatggtgca cattttccat ttcatttaga tttacctcct tctgcaataa gtttgggagc
      961 tgatatggta gctgtaagta tacataaaac tggaggagca ttgacacaat cttcagcact
     1021 cttattgaat agagataatg taagttttga aaaggttctt caatcaatca atatgctaca
     1081 atcaacatca gcatcttatt tgcttatggc aagtatagat ggtgctagag ttaatctagt
     1141 tgagaatggt gaaaagcaac tgtcaaaagc attaaatcta tctaggtatg caaaatctaa
     1201 attaaacaaa attgatggaa taaaagtttt atctacagaa atattgaaac aaaaaggtgt
     1261 taaatttata gatgaaacaa aattgtgtat aaatgtaaaa gaattaaatt tgactggatt
     1321 tgaagtatac gatttattat ataaaaattt ttcaattcaa gtagaattgg gagattcata
     1381 taatatactg gcacttgtgt ccattggaac aaataaatca gatatagata gattagtaaa
     1441 agcactttct ataattgcaa agatttatag aaaagaatca acattaaatg aatttaatat
     1501 ggttcaaata aatccaatta ttaaacttaa tccaagagaa gctttttatg caccaaagga
     1561 aagtgtagaa atcaattcat gtatagatag aatttgtgga gaatcaataa tggcatatcc
     1621 accaggtata ccgattattg ctccaggaga attgataaca gaagaaatta tggagtatat
     1681 tattttctta aaaaatagca atgcatatct tacagatgta caagataaaa acttagatag
     1741 aatattagta ataaaataaa ttactttatt ttgcaagggc aagtatttgt taagtagagt
     1801 ttatcaaata gaacttatta aaagtataaa ggagttgggg ttgaaatgaa atttgaaaca
     1861 ctaggaagac atatactagt agaatactat aattgtgatg aaaacgtact taaagaccca
     1921 cgtcttatag aaaattttat gaatgattca gcattaaatg caaaagcgac aattgtggat
     1981 tctgtattcc accattttaa tccttggggg gtttcaggag ctgttataat tgctgaatct
     2041 catttgacaa tacatacatg gcctgaatat ggttatgcag cagcggattt ttttacttgt
     2101 ggtgatatag acccatggaa aagttttgaa ttattagagc aacttttaaa gtctgaaaga
     2161 agcgagtcta cagaaattcc aagaggctta actacaaaaa ttaaaaaata ttcaaaaaaa
     2221 gatttaggta agattactca taaaccagaa gcagtataaa acatacaaga gaaagtgagg
     2281 attattatat ggaactttgg tatacagaag aatggacaga aaatgtaaga ttttctataa
     2341 aagtaaataa acatctattt gaaggaaagt ctcaatttca aaggatagat gtatttgata
     2401 gtgatgaatt tggaaaattt ctaacaatag atggattaat gatggtaaca tataaagatg
     2461 aatttatata ccatgagatg ataactcatg ttcctatggc tacaaattta aacataaaga
     2521 aagtattagt cataggtgga ggtgatggag gtacagtaag agaattaagc tgttatcctc
     2581 aaatagaaaa gattgacatg gttgaaatag ataaaatggt tgtagatgta tctaaggagt
     2641 atatggatat atgctcatgt aaattagatg ataaaagagt gagtttatat tttgaagatg
     2701 gtgttaattt tgttaagtgt gcacatgata aatcatatga cctgataata gtagattcaa
     2761 ctgacccaat aggaccaggc gaagggcttt tctcaactga tttttataag gattgttata
     2821 gaatactgac tgatgatgga attttagtaa atcaaagtga aagtccgtat tttgacttta
     2881 atgctaaaga gatgaaaaga gctaataaaa agttaaaaca gatatttcca atatcagaag
     2941 tttatcaagc acatatacca acatatccat ctggtcactg gttgtttgga tttgcttcaa
     3001 aaaaacttaa tccagtaaaa aatcaagata gaaatggatg ggagaaatta agtttaaaaa
     3061 ctaaatatta taatagtgat atacatttag gttcgtttat gttacctcaa tacgtgaagg
     3121 agatgctaga tgaagaataa tttctatcat aggaacactt ttatgagtat gaacaaaaat
     3181 tatgaagaat ctaatcttat agtatttggt gttggatttg atggaactac ttctaacaga
     3241 cctggggcta gatttgcaag tagctctatg agaaaagaat tttacggtct tgagacatat
     3301 agtccttttt tagatttgga tttagaggat tataatatat gtgattatgg agatttagaa
     3361 attagtgttg gaagtacaga acaagtctta aaagaaatct atcaagagac atataagatt
     3421 gttagagatt caaaggtacc ctttatgatt ggaggagagc atttagttac attaccagcc
     3481 tttaaagcag tacatgaaaa gtacaatgat atatatgtaa ttcattttga tgcccatact
     3541 gacttgaggg aagaatataa taatagtaaa aattctcatg caacagtaat taaaagaata
     3601 tgggatattg taggtgataa taaaatattt caatttggta taaggtctgg gacaaaagaa
     3661 gaatttaaat ttgctacaga agaaaaacac acatacatgg aaataggagg aatagatacg
     3721 tttgaaaata tagttaatat gctaaatgga aagaatattt atctaactat agatttagat
     3781 gtattggatg cagctgtgtt tccaggaaca ggtacaccag aacctggtgg tgtaaattat
     3841 agagagtttc aagagatttt taagattata aaaaactcta atataaatat agttggttgt
     3901 gacattgtag agttaagccc tgattacgat acaacaggtg tatcgacagt tatagcttgt
     3961 aaaatcttaa gagagttatg cttaataata tctgataaaa ttaaatagag tattttatat
     4021 aaatagtata tattataata taagaagtta ttttaaaaaa gttagtttaa gtttgacgct
     4081 tttctaaagt aacttcttat ttttgtaatt aaaaataaat ataataaaca acaaaaaaga
     4141 gccctcgata gctccttttc taaaatatat atatttaaat taaataatta tattctattt
     4201 acgttttcag cttgaggtcc tctattacct tgagttatat caaagctaac cttttcgcct
     4261 tcttctaaag ttttgtatcc atcattttgt atagctgaga aatgtacgaa aacatcatct
     4321 tctccctcta cagatatgaa cccaaatcct ttttcattgt taaaccattt tactattccg
     4381 tttttcatta taagaaaacc tccgcattat taaaaaatac aaactaaaaa aataagtctg
     4441 ttctattaat ataacataaa ttttaaataa aagcaaataa aatttttaaa aaaattaatt
     4501 acttttagaa tttaataaaa attgttaaat tatacagt
//
LOCUS       DAGSRM010000159         4534 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.165, whole genome shotgun sequence.
ACCESSION   DAGSRM010000159 DAGSRM010000000
VERSION     DAGSRM010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4534
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.165"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            125..424
                     /locus_tag="MJE33_003531"
     CDS             125..424
                     /locus_tag="MJE33_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649437.1"
                     /translation="MKWIKWYSITCICIFIVVAFYMFIFPNKIETIDTSSAYSFVEKK
                     VPNSAVYQGYKKNPVDGTTTIYYSYDNSTHIVRLSHPEDYSRKINWDKVSNIRFD"
     gene            748..2403
                     /gene="ilvD"
                     /locus_tag="MJE33_003532"
     CDS             748..2403
                     /gene="ilvD"
                     /locus_tag="MJE33_003532"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBY9649438.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSAM
                     EGVGACKDGTVSEEELEALAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRIAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIELNLDFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGKTIGENIRNCEIENEEVIHTLKNPYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFGKKINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKYVALLTDGRFSGATRGASIGHISPEAMEGGLIGLVEEGDIISINIPDKKLEL
                     KVDEVEIENRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
     gene            2747..3379
                     /locus_tag="MJE33_003533"
     CDS             2747..3379
                     /locus_tag="MJE33_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY9649439.1"
                     /translation="MNREEKSKNSKEKIIQSAFSLFSSKGYDSTSTQDIINLSGLSRG
                     AMYHHFKTKEDILRSVTKELYSQMNNFLEHLVADDTLTANEKIIELVVHSANDYTRRK
                     MVHCSWLEKIPFALIEEVRNLNNVVAPNIAKIIKQGVENKEFSCEYPEELAEMLVFSI
                     DILLDPVLFKREYSEVCNRLDFLLFMLKKMDIPLIDEYGIQKFKDLFRQL"
     gene            3402..4214
                     /locus_tag="MJE33_003534"
     CDS             3402..4214
                     /locus_tag="MJE33_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBY9649440.1"
                     /translation="MFYNAKSCQMMIGSNTVNYIEFGSGKKTLIILPGLGDGLFPLHG
                     KIQAIAFAFRYKQFTKDYKVYVFSRKNQITEKYSTRDMAKDQADIMKKLGIMKAEVMG
                     VSQGGMIAQYLAIDYPELVEKLVLAVTSSKQNDTIQNVICSWINMAKKQNYNDLMIDT
                     AKKSYSERYLKKYQLFIPFLGKVGKPKDFKRFIIQATSCIEHNAFSELNKITCPTLII
                     GGANDKIVGNNASFHLVEKIKKSEIFIYEGLGHATYEEAQDFNERVLEFLNK"
BASE COUNT         1764 a          502 c          814 g         1454 t
ORIGIN      
        1 aaatatatat aatcaaagtt tctacaatta tatagttagg gatataaaaa ttttatcgta
       61 tgtataaata ttataaatat atggaaataa taatagtata ttttggtaaa gtaggaggaa
      121 ttttatgaaa tggattaaat ggtatagtat tacatgcata tgtattttta tagtagtagc
      181 tttttatatg tttatatttc ctaataaaat agaaactatt gatacttcat ctgcatatag
      241 ctttgttgaa aagaaagtac caaatagtgc tgtgtatcaa ggatacaaaa agaatccagt
      301 tgatggtact actacaattt attacagcta tgataattca actcatatag ttagattatc
      361 acatccagaa gactattcaa ggaagattaa ttgggataaa gtttcaaaca tcagatttga
      421 ttaatcttta atatttataa aagtctatat atctaaggca aatatattat taatcttatt
      481 catagttgag cactttctat tatagaagtg cctcttctga tttatatcat aaaatattac
      541 ttaacaattt gtaaaatata ttagtttata aaatatattg aaaagataat attatataat
      601 acgattgtgt aagatattga taaaaaatcg taataaattt tcattagtat atgctaatat
      661 ttttttacaa ttttcgtaat taattaatag aaaaatatat tataataata ttatgaaaat
      721 ttaaaaaagt aagaaagagg taatattatg agaagtgata taaaaaaagg aatagaagga
      781 gctccaaaga gagcgttaat gtatggaatg ggtcttacta aagaagaaat tgaaagacca
      841 ttaataggta tagtaaatgc acaaaatgag gtgattcctg gtcatctgca tcttgatgaa
      901 atagcagaag ctgcaaaaaa tggagtaaga atgagtggtg gtttgccatt agaatttcct
      961 gctatagggg tatgtgatgg tatagctatg gggcatgtcg gaatgaatta ttccctggct
     1021 tcaagagaat taatagcaga ttcaatagaa gctatggcta tggcacatgg ttttgatgct
     1081 ttggtactta tacctaattg tgacaagata gtgccaggta tgttaatggc tgctgcaaga
     1141 cttaatatac caagtattgt tgtgagtgga gggcctatgc ttccaggtaa aaaaaatgga
     1201 aaagtatatg attttaactc tgcaatggag ggtgttggag catgtaaaga tggaacagta
     1261 agtgaagaag aattagaagc gttagccatg aactcttgtc ctggatgtgg ttcatgttct
     1321 ggtcttttca cagcaaatag catgaattgt ttaacagaag ctttaggcat gggtatacca
     1381 tataatggta ctgctgcgtc acattctgga gaaagaaaaa ggatagcaaa atatgcaggt
     1441 atgtatgtta tggagttact taagaacgac ataaaaccta gagatatttt aacaatagat
     1501 gcttttaaaa atgctatagc tgtggatatg gcaatggctg gctctacaaa tacagtactt
     1561 cacttacctg caatagctta tgaatcagga atagagctta acttagattt ttttgatgaa
     1621 ataagtgaaa aaactccttg tttaacaaaa ttaagtccaa gtggaaaaca tcatattgaa
     1681 gatttacata tggcaggagg aataccagct ataatgaacg aactttcaaa gataaatgga
     1741 ataaatttag attgcaaaac cgtaacaggc aagactatag gggaaaatat aagaaattgt
     1801 gaaatagaaa atgaagaagt aatacataca ttaaagaatc catatagtaa ccaaggtgga
     1861 cttgcaatat tgaaaggaaa tcttgctcta aatggagctg ttgtaaaaaa gtcagcagtt
     1921 gcagaagaaa tgttagttca tgaaggacca gcaagagttt ttaattcaga agaagaagct
     1981 gtaaatgcta tttttggtaa aaaaataaac aaaggtgatg ttatagttat aagatatgaa
     2041 ggtccaaagg gtggtccagg aatgaaagaa atgctatctc ctacatcagc agttgcagga
     2101 atgggacttg ataagtatgt agcacttctt actgatggtc gtttttcagg agcaactaga
     2161 ggagcatcta taggccatat ttctccagaa gctatggaag gtggtttaat cgggttagtt
     2221 gaagaaggag atataatttc tataaacata ccagacaaaa aattagagtt aaaagtagat
     2281 gaagttgaaa tagaaaatag aaaattaaaa tttaaacctt tagaaccgaa aataaagcat
     2341 ggatacttaa gtagatatgc taaactggta acatcagcaa atacaggagc agttttaaaa
     2401 tagtagacta tatttatgta aattagaaaa agttaagaaa atattaattg gatatcttag
     2461 gtgttatagc actaatgagg tatccttttt attttgtatg aaaaacacag caaaatcttg
     2521 attatctttg aaaacaatac atctctatta taaggaatac gagtacagca tagagatgta
     2581 tcttttagac cttgttgagg gaatacaact ttttaaaagt ttgatgtgga atgcttttcg
     2641 ttccatctaa actatgaaga aattgaaagt cagttaattc ttgacataat aaatattaaa
     2701 atgtataatg aaaaacaaac agacagtctg tttttaggag ggaaatatga atagagaaga
     2761 aaaaagtaaa aatagtaaag aaaaaattat ccaatcagca ttttcactat tttcgtctaa
     2821 aggatatgat tcaacatcta cacaagatat tatcaattta tctggtctat ctagaggtgc
     2881 aatgtatcat cactttaaaa ctaaagaaga tatactgaga agtgtcacaa aagaacttta
     2941 ctcacaaatg aataattttt tagagcatct tgttgctgat gacaccctta cagcaaatga
     3001 aaaaataata gaattggttg ttcatagtgc gaatgattac acacgtagaa aaatggtaca
     3061 ttgtagctgg ttagaaaaaa tcccattcgc tttaatagag gaagttcgta atctcaacaa
     3121 tgtagttgca cccaatattg ctaagataat taaacaaggt gttgaaaata aagaattttc
     3181 ttgtgaatat ccagaggaat tagctgaaat gctcgttttt agtattgata ttttacttga
     3241 ccctgtatta tttaaacgtg aatacagtga agtatgtaat aggttggatt ttttattatt
     3301 catgttaaaa aaaatggata ttcctctaat tgatgagtat ggtattcaaa aatttaaaga
     3361 tttatttaga caattataat aggaaaagcg aggaatagac tatgttttat aatgcaaaaa
     3421 gttgtcaaat gatgatagga agtaatacag tgaattatat tgaatttggt agtggtaaga
     3481 agacactaat tattttacca ggattaggag atggtttatt tcctttacat ggaaaaatac
     3541 aggcgattgc ttttgctttt agatataagc aatttacaaa agattataag gtttatgtat
     3601 ttagtagaaa aaatcaaatt acagaaaaat attccacaag ggatatggct aaagaccaag
     3661 cagatataat gaaaaaactt ggaattatga aagcagaagt aatgggtgtt tctcaaggtg
     3721 gaatgatagc tcaatatctg gcaattgatt atcctgagtt agttgagaaa cttgtgttgg
     3781 cagttacttc ttcaaagcaa aatgatacta tccaaaatgt tatttgtagt tggattaata
     3841 tggcgaagaa gcagaattac aatgatttaa tgattgacac agcaaagaaa tcctattctg
     3901 aaagatattt aaaaaaatac caattattta ttcctttttt aggaaaagta ggtaaaccaa
     3961 aggattttaa acgatttatt attcaagcga cctcttgcat agaacataat gccttttccg
     4021 aattaaataa aataacatgt ccaaccttaa ttataggagg tgctaatgat aaaattgttg
     4081 gaaataatgc ttcttttcat ttagtagaaa aaattaaaaa aagcgaaata tttatctatg
     4141 agggattggg acacgctaca tatgaagaag cacaggattt taatgaaaga gttttagagt
     4201 ttttaaataa gtaaacacct ttgttaaaaa tatacatatg aagattggaa atttaatgtt
     4261 aaaaatagaa acatgaaaat atgcttaact ggtattttta ctattcataa ccaatttttg
     4321 atacattatc tactataaat acaaatatag cttcaatgtg attatatatt gttgtattgg
     4381 taaagcactt atacaaacag aggaattttg taaattcata ttatacccac atttgttaac
     4441 ttatgaaaat ataatcaaaa tttttatgag ctttttatat aaaaaatata atgtttgtat
     4501 acaaaaatat taaagacatt ttagtaagtt ttgt
//
LOCUS       DAGSRM010000160         4518 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.166, whole genome shotgun sequence.
ACCESSION   DAGSRM010000160 DAGSRM010000000
VERSION     DAGSRM010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4518
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.166"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..2738)
                     /gene="carB"
                     /locus_tag="MJE33_003535"
     CDS             complement(<1..2738)
                     /gene="carB"
                     /locus_tag="MJE33_003535"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HBY9649441.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKEKPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIADTEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVVVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMGIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFRDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYLDDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYSTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEV"
     gene            complement(2931..3980)
                     /gene="carA"
                     /locus_tag="MJE33_003536"
     CDS             complement(2931..3980)
                     /gene="carA"
                     /locus_tag="MJE33_003536"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBY9649442.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESVGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITIFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            complement(4237..>4518)
                     /locus_tag="MJE33_003537"
     CDS             complement(4237..>4518)
                     /locus_tag="MJE33_003537"
                     /inference="COORDINATES: protein motif:HMM:NF014225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649443.1"
                     /translation="NSLRENGIEADIVNRVEESRPNILDAIRNKQVDIVINTPTKGND
                     STRDGFKIRRTAIEFSTEIMTSLDTLKALVEVKKKHLNKDELKVYNIAE"
BASE COUNT         1299 a          890 c          461 g         1868 t
ORIGIN      
        1 acttctcctg tagacttcat ttcaggtcct agacttactt caactttaga taatttagac
       61 attgagaata ctggtacttt tactgatact aattttggct ctttgtaaac tcctgtacta
      121 tatcctaaat cctttaattt agctcctaac atacacttag tagccaagtc aactattggt
      181 actccactta ctttacttat atatggaact gttctactag ctctagggtt aacctcaatt
      241 atatataatt cattttgaaa ttctataaat tgtatattaa ccataccaag cacattaagg
      301 tctaaagcta tttttttagt gtattctact attttttctt ttatttcatc agatatattt
      361 tgacttggat acattgttgt actatctcca gagtgaactc cagctctttc taaatgctcc
      421 attataccag gtatcaatat atcttcttta tcacatatag catctacttc tatttctcta
      481 ccagttagat atttatctat aagtactgga tttttatggt ctctatcaaa tgcatcatcc
      541 aaatactgaa ttagtttttc ttcgttataa gttatttcca taccttgacc accaagtaca
      601 taagatggtc taactaatac tggataacct aactccttag caatttcaac accttcatta
      661 gttgtccata caccttttcc ttttggtcta tttatatcta gtctctctag taaatcatca
      721 aatttttctc tatcttctgc tgcatctata tctctaaagt ctgttcccag tataggtata
      781 tttttttcat gtaaaaactt agccaatttt atagctgttt gtccaccaaa ctgtagtatt
      841 acaccttctg gtttttcttt ttctattata tttaaaactt cttcttctgt taatggttca
      901 aaatacaact tatcagatgt atcaaaatcc gtacttacag tttcagggtt attgtttaca
      961 attatagttt ctattcccat ttttcttaaa gattttacac agtgaactga acaatagtca
     1021 aattctattc cttgacctat tcttattgga ccagaaccca atactacaac ttttctctta
     1081 tcactaacta ctacctcatc atattgttca taagttgaat agtaataagg tgatagagct
     1141 tcaaattctc ctccacatgt atcaaccatt ttataaactg gttgtatatt atataaactt
     1201 ctcaattcat ataatttttc tgggcttatc ttcattaagt cagatatacc tttatcagaa
     1261 aatccctttt tctttaattc atgtaaatat tctttatcta agtcttctat ttttagaact
     1321 tttaactttt cttcttgttc tactatccat ttaaatttat ttataaacca cttatcaaca
     1381 ccagttattt ctgatatcat ttctacttta tagcctcttc ttatcatttc agctaagtca
     1441 aatagtctct catcatcagg tactactact ctagccttta attcttctaa tgttctacct
     1501 tctgatggag catgaaccaa tgaatatttt cctgtttcaa gtgatcttat acctttaaga
     1561 atagcagcct caaagttact tcctatactc atgatttcac cagttgccat catcttagtt
     1621 cctaattttc tattggcatg tctaaattta tcaaatggcc actttggtat ttttactacc
     1681 acataatcta atgttggctc aaaacaagca taagtttttt tagttactgc attttctatc
     1741 tcatccaaag tataacctaa agctatctta gctgctactt ttgctattgg gtatcctgta
     1801 gcttttgatg ctagggctga tgacctacta actcttggat ttatttctat tatcgcatat
     1861 tctaaactat gtgggtttaa agctatttga acgttacatc caccttgaac ctctattgca
     1921 tttattatgt ctattgatgc ctttctaagt agttgatact cttcatcact tagagtttga
     1981 cttggagcaa caactatact gtctcccgta tgaactccaa ctgggtctac attttccata
     2041 ttacatacag ttatacagtt tccatttcca tccctcatta cttcgtattc tatttctttc
     2101 caacctttta tactcttttc aagaagaact tgtccaacag gacttagctg tagtccatgt
     2161 gaaagtattt ctcttaattc ttcctcagta tcagctatac ctccaccagt tccacctagc
     2221 gtatatgctg gtcttacaac tactggatat cctatcttat tggcaaactc aagacctgct
     2281 tctaaatttg taactatatc actaactatt actggttgat ttatttcttt cattacttct
     2341 ctaaataaat ctctatcttc gccctttttt atagattcta tagatgttcc tataactttt
     2401 acattatatt tatctaatat accagcatca tgtaattcta cagctaagtt taatcctgtc
     2461 tgaccaccca tacctgcaag taaactatct ggtttttctt tttctattat tttttctata
     2521 aattctattg ttaatggttc tatatatatt ttatctgcaa tttctttatc agtcattata
     2581 gtagctgggt tactatttac taatacaact tcaattccct cttcttttaa tgcttggcag
     2641 gcctgtgttc ctgaatagtc aaactctgca gcttgaccta ttattattgg ccctgaccct
     2701 aatactaaag ttttctttat actatctaat ttaggcataa ctctttctcc ttatctaaac
     2761 aattttcaca gttcttatct tcatcatcta tacaatcaac tttaattact ttattttatc
     2821 ttaaattcaa atttactcat caatacatct atttaataca tctatgttaa ttaaaccaaa
     2881 ctttttgatt taagttattc ttgtttttta gttagctatt ctaatgttat ttattttact
     2941 aattctaaaa atttatcaaa aatatagtca ttatcttttg gtcctgggca agcttcagga
     3001 tggtattgaa cactgtatac atctagtttc tcatgtctca ttccttcaac agtattatca
     3061 tttaaattta tatgtgtcac tttcatttgt tcaggaactt ctgaaacata ataaccatga
     3121 ttttgagaag ttataaatac ttttccttct tctaaatctt taactggatg atttccacct
     3181 ctatgcccaa atttaagttt agcagttttt cctccaagag ctaaagctaa aagttgatgt
     3241 cctaagcata ttccaactat aggtttttta ccaataagag cttttatatt ttctataaca
     3301 tcttctaggt cttctgggtc tccaggtcca tttgacaaga atattaagtc agggtttata
     3361 ctcaatacat cttctggaga tgttgtagct ggaaatattg ttatatcaca tccacgagct
     3421 ataaatgatc ttaatatatt tctttttact ccaaaatcca taacagccac ttttggacca
     3481 tttcccttta tatgctctac ttcttttctt gtaacagttc taactgcttc tgtatttgaa
     3541 aacttatcca atttatgttt aacatcttct aatttactgt cttctaatgt aattatacct
     3601 ttcatggttc cattatttct aagtatctta gtcaaagctc ttgtatctat tccttcaagt
     3661 ccgataactt tattttgctt taaatattgg tctatatcaa tttcacatct aaaattactt
     3721 ggagaatcac ttttctccct tactatgaat ccctttactt gaactttttc agactctgct
     3781 gatgataaat ttataccata atttcctaca agaggataag tcatagtaac tatttgacca
     3841 tagtaagaag ggtctgttaa aacttcacca tatcctatca ttgaagtatt aaatactact
     3901 tctcctacac tttcttctaa atatccaaat gcctttccta taaaaactgt tccatcttct
     3961 aatattaacc ttgctttcat actacagtac ctcctataat tctctttaga caaactaaag
     4021 aacattaact aaatataagc ttttaaatat ctatatctat ttttcacttt tacattctag
     4081 ttttatactt atttacattt attttttatc tttttctatt atactacaaa tttcttaaaa
     4141 aaaagaagtt atctcaaaag taaaaactcc tagaaaaaat aacctctttt ttttatttaa
     4201 taattaaatt cttaaactat acaatcaact taatttctat tcagctatat tgtaaacttt
     4261 taactcatct ttatttaaat gtttcttctt aacttctact aaagctttta gtgtatccaa
     4321 agatgtcatt atttcagtag aaaattcaat agctgttctt cttatcttaa atccatctct
     4381 tgtagagtca tttcctttag ttggtgtatt tattactata tcaacttgtt tatttcttat
     4441 agcatctaat atgtttggtc tagattcttc tactctattt actatatcag cctctattcc
     4501 attttctctt aagctatt
//
LOCUS       DAGSRM010000161         4462 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.167, whole genome shotgun sequence.
ACCESSION   DAGSRM010000161 DAGSRM010000000
VERSION     DAGSRM010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.167"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     misc_binding    86..320
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            370..1389
                     /gene="pheS"
                     /locus_tag="MJE33_003538"
     CDS             370..1389
                     /gene="pheS"
                     /locus_tag="MJE33_003538"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBY9649444.1"
                     /translation="MQEKLLALREAALAEIKEAQSIESVESLRVKYLGKKGEITAILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     gene            1406..3799
                     /locus_tag="MJE33_003539"
     CDS             1406..3799
                     /locus_tag="MJE33_003539"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBY9649445.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMSGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAVNGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     KDALIDFELTSNRPDCKCMMGIAREAAATIGTKVKYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRNAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITSN
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAAQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVANDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNAKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFALKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSITYRSKDKTLTDEDVAKVHDKILSELSEKLNANLRSN"
     gene            3824..4414
                     /gene="zapA"
                     /locus_tag="MJE33_003540"
     CDS             3824..4414
                     /gene="zapA"
                     /locus_tag="MJE33_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBY9649446.1"
                     /translation="MNKVMVKIHGAEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAETKMKELIEIIENKKQEIFELSNTTEGSRAELDAYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDAKNVE"
BASE COUNT         1754 a          519 c          919 g         1270 t
ORIGIN      
        1 attaattatg tattggatat atattaaaaa tagtcacata atttgaatgg aaatgatata
       61 atactaaaat aaacaatata atattgtaaa tgcaatgaaa gaggaaagta ttttgattaa
      121 acttagtaaa gagataaaca cctaggctgg gagtgttttc taagaggtca tgagaagttc
      181 cctctggagt aacagagctg aaattttaca gtaggctttg acgtcaaaaa cgcgttaagt
      241 ttgttagagg tggtttgatg atttttaaat tgttaaacta ctagggtggt accgcgaaac
      301 tatagccttt cgtccctaga cagggattga ggggcttttt ttatacaaaa aaacgaaagg
      361 gtgatatgtg tgcaagaaaa attacttgct ttacgtgaag cagctttggc tgaaataaaa
      421 gaagcacaaa gcatagaaag tgtagaaagt ttaagagtta agtacttagg aaaaaaaggt
      481 gagataactg ccatacttaa agaaatgggt aaattatctg ctgaagaaag accagtagtt
      541 ggtaaggttg ccaatgaggt aagagaaaac attgaactta gcataaattc taaaaaagaa
      601 gaaataaatg ctattgaaaa agaaagaaaa ttaaaagagg aagtgataga tgttactcaa
      661 ccaggaaaag ttttaaaggt tgggaagaag catccaataa ctcaaattat agatgaagta
      721 acagatatat ttatcggaat gggattctct atagcagaag ggccagaagt tgagactgtt
      781 gaaaacaact ttgacgcatt aaacgctcct aaagaccatc catcaagaga tatgagcgat
      841 acattctata tcaatgatgg ggtattactt agaactcaaa catctccagt tcaagtaaga
      901 actatgagaa gtcaagagtt accaataaaa gtaattgcac caggtagatg ttttaggtca
      961 gactcgccag atgctacaca ctcaccaatg ttccatcaga tagaagggct tgttgttgga
     1021 aaagatgtta ctatggcaga atttaaagga actatggata tcttcgttga aaaattgttt
     1081 ggttctgata tcaaaactaa gtttagacct cacaacttcc catttacaga accaagtgca
     1141 gaggttgatg ttacttgttt caaatgtggt ggtaaaggtt gcccaatgtg taaatatgaa
     1201 ggttggatag aaatattagg ttcaggtatg gttcatccaa atgtgcttag aaattgtgga
     1261 atagacccag aagtttacag tggatttgca tttggagttg gggttgaaag acttgcaatg
     1321 cttaaatatg aaatagatga tattagatta ttattcgaaa atgatatgag attcttaaat
     1381 caattttaat taggagggta tgtagatgtt agtatcttta aaatggctta gagactatgt
     1441 tgatatagac atggatgtaa aagagttcgc tgataaaatg acaatgtcag gaactaaagt
     1501 tgaaacaata gattattatg gtgaagaaat agaaaatata ttggttggaa agattttaga
     1561 aataaaacaa catccaaatg ctgataagtt ggttgtaacc aaagtagata ttggagataa
     1621 agttgttcaa atagttacag gagctacaaa tatatcagaa ggagattata ttccagtagc
     1681 tgtaaatggt tctaagttac ctggaggagt tgaaatcaaa cagactgatt tcagaggtga
     1741 attatcagat ggtatgatgt gttcagcagc tgaactaggt atagatgaac attacattga
     1801 ggagtataaa agaggtggta tatatatttt agaccacgaa gattcttatg aattaggaaa
     1861 agatataaaa gatgttttag gattaaaaga tgctttaata gattttgaat taacttcaaa
     1921 cagacctgat tgtaaatgca tgatgggtat agctagagaa gcagctgcaa ctataggaac
     1981 aaaagtaaaa tatcctgaaa tcgaagtaaa agaaagtgac gaagagatag atttcaaagt
     2041 tgagatagat aatccagatt tatgtagaag atatgttgct agaatggtta cagatgtaaa
     2101 aatagaacct tctccatatt ggatgcaaag aagacttaca gaagcaggag taagacctat
     2161 aagtaacata gtcgatataa caaacttcgt aatgttagag cttggtcaac cacttcatgc
     2221 ttttgatata aatcaagtag agactggaag aatagtagta agaaatgcta aagatggaga
     2281 gaaacttgta acattagatg atgttgagag aacattagac aaagatatgc tagttataac
     2341 aaatggagaa aaatcacttg gtttagctgg tgtaatgggt ggtgctaact cagaaataac
     2401 ttctaatacg aagactgtac tttttgaaag tgccaatttc aaaccagaaa acataagaat
     2461 gacagctaaa aaagttggta ttaggtcaga agcatcttca agaaatgaaa aagacttaga
     2521 ccctaatctt gcagagatag cagcaaatag agctgcacag cttgttgaaa tgttaggagc
     2581 aggaaaagtt ttaaaaggtg ttgtagatgt atatccaaat aaaccagaac ctaaaaaatt
     2641 ggtagtaaat cctcaaagaa ttaaccatct attaggtgta gatgtaccaa tggagcagtt
     2701 tgtaggaatt ttagaatcat tagagtttaa atgtaatttg gtagctaatg ataaattaga
     2761 aatagatgta ccaagcttta gaacagatat ggaacaagaa gctgatgtat gggaagaaat
     2821 agctagaatt tatggatttg agaatatacc ttctgtacaa ttagaaggaa atacgacagc
     2881 aggaattaaa acttcaaaac aaaaattcat ggatgcttta aaagataatt caactgctgt
     2941 aggattaaat gaaatattaa catattcatt tgtaagccct aagggagtag ataagataag
     3001 agtaccagaa ggtaatgcaa agagaaattt tgttaaatta cttaacccat taggagaaga
     3061 aacttctgta atgagaacta ctttaatacc aaatatgtta gatgttttat caactaatgt
     3121 atctcataaa atagaagaag tatctgcatt tgagtgtgga catatattca tcccacaaga
     3181 ttcagaatta cctaaggaag aaaatagaat gtgtgtaggt atgtatggta aagatgttga
     3241 cttctttgca ctaaaaggaa ctatagaaac tatacttgta aatgtcggat ttaaaggtta
     3301 tgaaattgaa cctcaagata ataatactac cttccaccct ggtagatgtg ctaaaatagt
     3361 gtataataat aaatatgtag gtacattagg tgaattgcat ccagatgtta tagaaaacta
     3421 caatctaggt caaagagttt atgtcgctga aatagatatt gactttgtat ttgataattc
     3481 tgatagaaca aagaattatg tgccattacc aaaatatcca tctacatcaa gagatatagc
     3541 tcttattgtt aaagatgatg tatttgtaaa acaaatagaa gatataataa aggaaaatgg
     3601 tcaaggatta gtagaaagct acaagttatt tgatgtttac aaaggttctc aaatagaagc
     3661 tggatacaag tcaattgcat attcaataac ttatagaagt aaagataaaa ctctaacaga
     3721 tgaagatgta gctaaggtgc atgataagat attaagtgaa ttaagcgaaa aattaaatgc
     3781 taacttaaga tcaaattaac aactaatgtc gggggaggtt aggatgaaca aagtaatggt
     3841 taaaatccat ggcgctgaat atccaatggt tggagacaaa tcagagaagt ttatgattag
     3901 tattgctgat tttgtagaca aagaaatgga taaaataaca cgtcaaaacc cgaaattgag
     3961 cttatcagtt gctgcgatat taactgcatt aaatatatca gaccttttat ttgaatgttc
     4021 agatgagaat gagaagttga ttaaagctaa tgaagaattg agtaagaagg ttggagcatc
     4081 taatgaagag cttcaattag aaatcaagtc tttaaagctt acgatagcag aaaaagaagc
     4141 agaaaatcgt gaagctgaaa ctaagatgaa agagttaatt gagattatag aaaataaaaa
     4201 gcaagaaata ttcgaactta gtaatacgac agaaggttct agagcagagt tagatgctta
     4261 taaaaacaag attgaggagt taagtactca acttgaagaa gcaaatgaaa gagctactat
     4321 agctgaaaac ttagcttctg agtttcaaaa caaagcttat gatttacagt taaaatgtac
     4381 agggttaaat aatgatgcaa aaaatgtgga ataaactaaa agatttgctg tctcttgttt
     4441 tttgagacag cttcttttaa tg
//
LOCUS       DAGSRM010000162         4422 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.168, whole genome shotgun sequence.
ACCESSION   DAGSRM010000162 DAGSRM010000000
VERSION     DAGSRM010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.168"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            516..1772
                     /locus_tag="MJE33_003541"
     CDS             516..1772
                     /locus_tag="MJE33_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBY9649447.1"
                     /translation="MIEKFKTLIWIKMIIKIKSVYKTYGGLFLLGLIGIPIGAIIGLI
                     DTIFGTVLLKVTDIRETYPMYLIPFLAVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGE
                     EEIIPLRLVPFIILGTWLTHLFGGSAGREGVAVQIGATFSHWVGKRLPIKNASSIFLV
                     TGMAAGFAGLFETPIAAILFAMEVLVAGSLEYQSLFPAFTASFTASAVSKALGLEKFS
                     FALSSKVVFDLSIFWKLIVLGIIFGMVGGAFAWCLKLSKRKIGNRLKNPMIRIAIIGV
                     CLSVLFLLFYKGRYSGLGTNLIQNSFYGGEIYSFDWLLKFILTILTLSAGFQGGEVTP
                     LFSIGASLGVLLAGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYF
                     FVVCAISYIFNMDKSIYSLQKISTKQ"
     gene            1835..3028
                     /locus_tag="MJE33_003542"
     CDS             1835..3028
                     /locus_tag="MJE33_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBY9649448.1"
                     /translation="MLTSLALIFLLGMASGGIFKRIKLPNLLGMLLTGIILGPYVLNL
                     IDNSILDISSDLRKIALIIILTRAGLSLDINDLKKVGRPAVLMCFIPATFEIVGMIVL
                     APKLLGVSILEAAVMGAVVGAVSPAIIVPKMLKLMEEGYGTEKSIPQMLLAGTSIDDI
                     FVIVMFTVFTGLAQGNSISAISFLQIPVSIILGVIVGAVIGICLAVFFKKIHMRDSAK
                     GVLLLSISFLMISLETALEGIVPFSGLLAVMSIGIFLQIKYRVVARRLSIKYSKLWVG
                     AEILLFVLVGATVDISYAFKAGIGAVILIFGVLLFRMVGVFFCLIKTNLTIKERLFCM
                     IGYIPKATVQAAIGGVPLAMGMASGQLILTLAVLAILITAPLGAFGIDVTYKKLLTSV
                     KGENK"
     gene            3229..3609
                     /locus_tag="MJE33_003543"
     CDS             3229..3609
                     /locus_tag="MJE33_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBY9649449.1"
                     /translation="MCLKKLSKLELVIMKFIWNLDIKTNSYEIIDYMKEEHNLPEKVA
                     LKTLSKLSKKRFLYVQETGKCMYYTVAIKEKAYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEELTEEKIKSLENWVVNWEEAYV"
BASE COUNT         1589 a          438 c          756 g         1639 t
ORIGIN      
        1 tatcataatg aaaagtaaag aaaatcattt aaattatata tcataaaaat aatatgaaat
       61 attgaacact cataatatta gagactttaa tattttaatt gataggttat attatttata
      121 acattaaaaa tacaatttac ataaaataga catatgaaaa ataaaaaatt atataataat
      181 tatgggaatg aggttctccc ttggatgata atatcctaaa ctgccaatga gctgatgact
      241 tctatgatat catagatgcc accagctttt tttgttgttt aaatcgataa aatggaggtg
      301 aattacttac taaattttta ttttttaaat ttgaagaaat atttaatcaa taaaaaaaat
      361 agtagattat atgttaatta ttttattaag aatgggttaa gagtgtacct atatcctatc
      421 aattaaaaaa acaattacga tagtcattac attgactaca gttgatgcaa taaaactttc
      481 aaaaaatctt atgatgcaaa tagaaaggaa ttaaaatgat agaaaaattt aaaactttaa
      541 tatggattaa aatgataata aaaattaaaa gcgtttataa aacatatggt ggactatttt
      601 tgttaggact cataggtata ccaattggtg caattatagg tcttatagac accatatttg
      661 gaacggttct tctaaaagtt acagatattc gagaaacata tccaatgtat ctgataccat
      721 ttttagcagt agtaggagtt gttattgctt attgctattt taaatttgga ggaaaaagta
      781 gtaaaggaat gaatctaata tttgaggtag ggcatggaga agaagaaatt attcctttaa
      841 gattggttcc atttataata ttaggtacat ggttaaccca tttatttggt ggtagtgcag
      901 gacgagaagg agttgctgtt cagattggtg caactttttc acattgggta ggtaaaaggt
      961 tgcctataaa aaatgcttct agtatttttc tagtaactgg tatggcagct ggttttgcag
     1021 gattatttga gacaccaata gcagcaatac tatttgctat ggaggttttg gtagctggtt
     1081 cactggagta tcaatcgctt tttcctgcgt ttactgcttc ttttacagca agtgcagtat
     1141 caaaagcttt agggttagag aaattttctt ttgcactttc ttctaaagtt gtatttgact
     1201 tgtcaatatt ttggaagctt attgttttag gaattatttt tggaatggtt ggtggagcat
     1261 ttgcatggtg tctgaaattg tcaaaaagaa aaatagggaa tagattaaaa aatcctatga
     1321 taagaattgc tattattgga gtatgtctta gtgtattatt ccttttattt tataaaggaa
     1381 gatattcagg tttagggaca aatttaattc aaaatagttt ctatggtgga gaaatttatt
     1441 catttgactg gctgttaaag tttatactta caatactgac tttgtcagca ggttttcaag
     1501 gaggagaggt tacacctttg ttttccattg gagctagtct tggtgtattg ttagcaggat
     1561 tttttaattt gccaattgaa ttagttgcag cgcttggcta tgctagtgtc tttggtagtg
     1621 ctacaaatac tttttttgca cctgtattta taggggcaga agtttttgga tacagctatt
     1681 taccttattt ctttgtagta tgcgctattt catatatatt taatatggat aaatctattt
     1741 attcactaca aaaaatttca acaaaacaat aaataaaaca acataatgta tgattagaag
     1801 agaaagaata ttagagaaag gatagggtag gaatatgttg acaagtttag ctttgatttt
     1861 tttattagga atggcctcgg gaggtatttt taagaggata aagttaccta acttattggg
     1921 aatgttactt acaggaatta tattaggtcc atatgtttta aatttgattg ataattcaat
     1981 actagatatt tcttctgacc ttagaaagat tgcattgatt attatattaa caagggctgg
     2041 attatcttta gatataaacg atttgaagaa agtgggaaga cctgctgtac ttatgtgctt
     2101 tattccagct acttttgaaa ttgtcggaat gatagtttta gcaccaaagt tactaggcgt
     2161 ttctatattg gaagcagcag tcatgggtgc agtagtagga gcagtttctc ccgcaattat
     2221 tgtacctaaa atgcttaaat taatggaaga aggttatggt acagaaaaaa gtattccaca
     2281 gatgctttta gcaggaactt ctatagacga tattttcgta attgtgatgt ttacagtatt
     2341 cacaggattg gcacaaggaa atagtatatc agctattagt tttttacaaa ttccagtatc
     2401 aattatatta ggtgtaatag ttggagcagt gattggaatc tgtttagcag tattttttaa
     2461 gaagattcat atgagagatt ctgctaaagg agtgttgctt ttaagtatct catttttaat
     2521 gataagttta gagactgctt tagagggtat tgtgccattt tctggattat tagcagttat
     2581 gagtattggg attttccttc agataaaata tcgtgtagtt gcaagaagat tgtctataaa
     2641 gtattcgaaa ctatgggttg gtgctgaaat tctcttgttt gttttggtag gggcaacagt
     2701 agatataagt tatgcattta aagcaggaat tggagctgta attttaatat ttggagttct
     2761 tttattccgt atggtaggag tattcttctg ccttattaag acaaatttga caataaagga
     2821 aagattgttc tgtatgattg gctatattcc aaaggctaca gtacaggcag caataggagg
     2881 tgtaccactt gcaatgggga tggcatctgg acaacttatt ttgacgttag cagttctagc
     2941 tatacttata actgcacctc ttggagcttt tggaattgat gtaacttata aaaaattatt
     3001 aacttctgta aaaggcgaga ataagtaaaa ataaagatta ttttaactgg taagttatat
     3061 tttggaaaag gattatacta ttgaatacaa tataaaaaaa tcatttggga gattaagaag
     3121 gtagaagtta agaacagatt ttaaggtatg ttatgattat tagaattgat tttgtgaaat
     3181 tattattgta atatttaatt atatagataa agaagcggag tgataactat gtgtttaaaa
     3241 aaattatcaa aattagagtt agttattatg aaatttattt ggaatttaga tataaaaacc
     3301 aattcatatg agattattga ttatatgaaa gaagaacata atttaccaga aaaagtagct
     3361 ttaaaaactt tatcaaaact atcaaagaaa agattcttat atgttcaaga aacaggcaag
     3421 tgtatgtatt atacagttgc aataaaagaa aaggcatatt tagagtttat atcaagaaat
     3481 gtgcaaaatc ttttgaagaa taattttata aggaatttat tagtatcatt tcacgaagag
     3541 gagctaacag aggaaaagat aaagtcgtta gaaaactggg tagtaaattg ggaagaagct
     3601 tatgtataaa tgttattatt tttattacaa tttagataat tttggttttg taagctctgc
     3661 aactattttt agatggttgc agagtttatt tttatttttt attcccttaa gcaatactta
     3721 aaagtaaacg catttatatt tactaacttc aattacttat aatacttaaa ttacttttta
     3781 aaaatatttt gatatgtcat ctctatagta atctagaact tgtttctatt ctttatataa
     3841 agtttttatg aatgatatat ataatattcg ttcatttgat taaaaataac aaaatattaa
     3901 ataattctac tctgatagtt tgttaaaaaa ataataaaaa atattaataa acaaaaaaaa
     3961 attatcttaa gagaggagaa tgttctaaaa tataaaaagg tttctagatt tcataaaaga
     4021 tactatttta gtcttgaaaa tatttagttt gaaaagattt taatttaatg attgattaag
     4081 ttaaaaatgt gtatgtaata aattttattt tatttattga ctaaattata aagtttacat
     4141 aattatttaa taattatgta attgttactt gaaaattgat ctattttaaa atctagttat
     4201 aacttcaaaa aagactgaaa attaagaaaa aagaaatata aatataaaaa tatgtcatat
     4261 agattttttt tattttactt taataaaatg atttgttttt acaatacttt attaatataa
     4321 agtttattgc taaaatactt tatttattag aaaaagatta ctaattaatt attaaaatta
     4381 atgtattcat aatgcatatt ttcatataaa atttaattta tt
//
LOCUS       DAGSRM010000163         4219 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.169, whole genome shotgun sequence.
ACCESSION   DAGSRM010000163 DAGSRM010000000
VERSION     DAGSRM010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.169"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(213..1130)
                     /locus_tag="MJE33_003544"
     CDS             complement(213..1130)
                     /locus_tag="MJE33_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HBY9649450.1"
                     /translation="MVNMNIIEIRSDKIYKKIMDAPINKKEDIYRYELMKPFEFKWKC
                     MNVPIVARQKGGYDVIIASEMLGVLSPKDIDEKQKKNINVLSADKIWGTCKETIENSI
                     NAFIKEGYDLNIKDYKYSILLANPNSPYTILSDGYWGDGGIPGYIFLSLVPNEYTINR
                     LPVLIAHECNHNIRFQFIEWNNNITLEEMMINEGLAENFATWMFGEEMLGPWVSRTDI
                     ETLNTYIKPIIKSALKETGFRNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKY
                     YLEKTNKSIIEATLLPYSEIIEAVKEFWE"
     gene            1392..1769
                     /locus_tag="MJE33_003545"
     CDS             1392..1769
                     /locus_tag="MJE33_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBY9649451.1"
                     /translation="MLTPYLIFNGTCEKAFNFYAEAFGGGKTIFARLDSNPNNPIMHA
                     SVTFTKYEGCIMGADTDKPVVISGMAICVVLPSREAIEEISVKLAEGGTLVQEFLPHP
                     PPHQNDGAAEVLDRYGYTWYLST"
     gene            complement(1925..2374)
                     /locus_tag="MJE33_003546"
     CDS             complement(1925..2374)
                     /locus_tag="MJE33_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY9649452.1"
                     /translation="MKSGIILEMKQINSTEYDEMLQVWESSVRATHDFLTEKDIESLI
                     PLVEIGLKEVENIVCIKDNDIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKVFDRSEIDGQGNPFPILHMKLI"
     gene            complement(2538..2942)
                     /locus_tag="MJE33_003547"
     CDS             complement(2538..2942)
                     /locus_tag="MJE33_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HBY9649453.1"
                     /translation="MSYQNYKCERDYVYDDYKYNKCNKCYNDYEEMTHVHEYSESVKL
                     AEECEDRHNHRAAGVTGEAIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGT
                     DKHIHLICGETTVNDGHCHKFLFTTQIEAPLV"
     gene            complement(3055..3921)
                     /locus_tag="MJE33_003548"
     CDS             complement(3055..3921)
                     /locus_tag="MJE33_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBY9649454.1"
                     /translation="MIDIHKNIYDNKLFEELKIDCKKCFGLCCVALYFSASDGFPIDK
                     ESGKPCINLQPDFKCSVHNSLMKRGFKGCTAYDCFGSGQKVAQVTYKGIDWMQSPELT
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKIDLIIEETEKITNMGPEQIINLD
                     IISHRTKVNLLLSQASESVMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKINLIGAD
                     LRGRFLIAANLRNTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PASLIRPKYWEK"
BASE COUNT         1401 a          695 c          461 g         1662 t
ORIGIN      
        1 tatataacta cgaaatataa agtaattaga taagtaacaa gaaactaaca tgtaatgtta
       61 ggtactctag atatgaaatc ttaaaacttt taaatattga aatatttctt tatatatatt
      121 attgtatatt tcttataaat tattgtcaat ttattttttc ttaaaaataa aataattgca
      181 taatttattt caaattatgc aatttataaa atttattccc aaaactcttt tactgcttcg
      241 attatctcac tataaggcaa taaagtcgct tcgattattg atttatttgt cttttctaaa
      301 taatacttaa tcatataata tccacaagca tatcctgcac aataaggcaa ccctactgga
      361 aaatatcctt gcattttagc tatatcatca ccataaagat aagatgttat atttcgaaat
      421 ccagtttctt ttaaagcact ttttattatt ggctttatat aagtatttaa tgtttcgata
      481 tctgttctac tgacccaagg tcctaacatt tcctctccaa acatccatgt tgcaaaattt
      541 tctgcaagac cttcatttat catcatttct tctaatgtta tattattatt ccactctata
      601 aactgaaatc taatattgtg attacattca tgtgctatta atactggtaa tctattgata
      661 gtatattcat taggaaccaa tgatagaaat atatatccag gaatcccacc atcaccccag
      721 tatccatcac ttaatattgt ataaggacta tttggattcg ccaataatat tgaatattta
      781 tagtccttaa tgtttaaatc atacccttct tttataaaag catttataga gttttctatg
      841 gtttctttgc aagtgcccca aattttatca gcagataaca catttatatt ctttttttgc
      901 ttttcatcaa tatccttagg cgataaaacc cctaacattt cacttgctat aattacatca
      961 tatccacctt tctgtctagc aactattgga acattcatac acttccactt aaattcaaaa
     1021 ggcttcatca attcatatct gtatatatct tcttttttgt ttattggtgc atccattatc
     1081 ttcttgtata ttttatctga gcgaatttct ataatattca tatttaccat ctcctttaag
     1141 acaaagtata tacaattacg tagcgtcaat gtcaatatac attttttata atgtcacctt
     1201 aatcttttta ttatatttta tcctgtaaac cttccgtaca tataacattt ttttaaatta
     1261 ataaatttgt ataatataat aataaaaaat tatatacttc aaggtttccc tatgatacaa
     1321 tttactaaat cataatttag attatcaggt gcaactataa attattctat atcaaggagg
     1381 atatattggt catgctaact ccatatttaa tatttaatgg tacttgtgaa aaagcattta
     1441 atttttatgc tgaggctttc ggaggaggaa aaactatatt tgcgcgatta gacagcaatc
     1501 caaacaatcc tattatgcac gcaagtgtta ctttcacaaa atatgaaggt tgtataatgg
     1561 gtgcggatac agacaagcct gttgtaattt ctggcatggc gatttgtgtt gttctaccat
     1621 ctcgagaagc gatagaagaa atatctgtaa aacttgccga aggtggtaca cttgtacaag
     1681 aatttttacc acacccacca ccacatcaaa atgatggcgc tgctgaagta cttgataggt
     1741 atgggtatac ttggtattta agtacatagt taaaataaaa aaactacatc taataatcat
     1801 aatatagaac agataaattt caatttaata gtgtagtaaa aaagtcgtgt cctaagactg
     1861 tcacgacttt tatttgtata aaaatttaag taaatatttt tgcgaataaa tgagaagtat
     1921 aactttaaat taatttcata tgtagtattg gaaatgggtt accttgtcca tctatttcag
     1981 acctatcaaa cactttaaat cctaagtgaa tataaaatcc tacagctttt ttattctgct
     2041 catttacatc aacatatttt acattgtatt tatctatagc atattttatt aattttttac
     2101 caataccatt tcctctatat ttatcttcta taaagagcat ctcaatttta tctttatcta
     2161 ttcctataaa gccctttatt atatcattat cttttataca aactatattt tctacttctt
     2221 taagtccaat ctcaactaat ggtatcaaag attcaatatc tttttctgtt aaaaaatcat
     2281 gcgttgctct tactgatgat tcccagactt gcaacatttc gtcatattct gttgaattta
     2341 tttgtttcat ttcaagaatt attccagatt tcataaaaat ttcctcccta tatcactttt
     2401 aattgcataa cactttttta tacaaaactt agaaaggact acgaaggtta aatatcatag
     2461 tcctcattaa gtttaaatat tatctattat atattatact ataatctagc aaattcacta
     2521 caataattca catcagctta tactaaaggt gcttctattt gagtagtaaa aaggaattta
     2581 tgacaatgac catcattaac agtagtttct ccacatatta agtgaatgtg tttatcagtt
     2641 ccaggtattc taattgctgg ccctgttgta acacatatct tatggaaatg gtcaaggaaa
     2701 tctacattat catttatttt atgaacatga tttgtacctc catttattgg tatagcttca
     2761 ccagtaaccc ctgctgcacg gtggttatgt ctatcctcac attcttctgc taacttaaca
     2821 ctttctgaat attcatgaac atgtgtcatt tcctcataat cattatagca cttgttgcac
     2881 ttattgtatt tataatcatc atacacataa tctctttcac atttataatt ttgataactc
     2941 atttttatat ctctccctat attatattaa gaaattcttg taaagaatct ctaatgtata
     3001 atatgaatta aagtcaaaaa agttcatatt ttgtaaaatt atgtattaat ttaattattt
     3061 ttcccagtat ttagggcgta ttagtgaagc tggtaacttc gtactagaat ctccttttgc
     3121 agtatttact tgtaattgtg ttaaaaatat actattttca agattagcac ctctaatatc
     3181 acaatctctt aagtctgctc ctattaaatt tgccccactc aaatctgtat ttcttaaatt
     3241 tgctgctatt agaaatctac ctcttaaatc tgctcctatt agatttattt ttctaaggtc
     3301 tgctcctatt aaatctatat ttttacttaa ttttttcatg tttttaagag tagaagtctt
     3361 gataaatgat ttaacctttc ccatcacaga ctcactcgct tgtgataata ataaattaac
     3421 ctttgttcta tgtgatatga tatccaaatt aattatctgt tctgggccca tattagttat
     3481 cttttcagtt tcttcaatta ttaaatcaat ttttgatttg attgtatctg atatatttaa
     3541 tacagatgct tctttaagat accataacat ttcatgtaat tgtctcataa ttaaaaacac
     3601 ttcagacatt tgatttgtta gttctggtga ttgcatccag tctatcccct tatatgttac
     3661 ttgtgctact ttttgacctg aaccaaaaca atcatatgca gtacaccctt taaaacctcg
     3721 cttcataaga ctattatgta cagaacattt aaaatctggt tgtaaattaa tacaaggttt
     3781 tcctgattcc ttatcgatag gaaatccatc tgaagcagaa aaataaagag ctacacagca
     3841 aagtccaaag cacttcttac agtcaatctt tagctcctca aataacttat tatcatatat
     3901 atttttatgt atatctatca tattttttct cctcttaaac tttattttta atattatatt
     3961 atctataact aaatgataat caagtccctt atttaacagt tcttattctt aattataatt
     4021 taaggtatta tttattccaa tagcatacat aagtttcttt atacttgtaa caataccttt
     4081 atactaagac ttattttaaa tgtagcactg taataataaa ttgtttatat actcataatg
     4141 tatttgataa attgtaaatc tttatagaag ttgaaatttt taatgatttt agaatcaaga
     4201 tataataata aatacaatt
//
LOCUS       DAGSRM010000164         4100 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.170, whole genome shotgun sequence.
ACCESSION   DAGSRM010000164 DAGSRM010000000
VERSION     DAGSRM010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.170"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            <1..392
                     /locus_tag="MJE33_003549"
     CDS             <1..392
                     /locus_tag="MJE33_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBY9649455.1"
                     /translation="KIEHVVITNNNIENYINFYKKFGFSSKEMSKNYKLNARNVKINL
                     NIKGYKFLPYVKNSKASRDNLCFKINTNIDEPKKYLEQNEIQIERGIVECTGVFGNIK
                     SIYLKKTNGNLIELSNHRITESKPWKI"
     gene            380..1318
                     /locus_tag="MJE33_003550"
     CDS             380..1318
                     /locus_tag="MJE33_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBY9649456.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFKLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVILEIFRGGKVNIKH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLHKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHINPIILGVCGKLILVPAIFLPISIFLGFRNVELASLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSGFSIITVFLIIFILKQNNLI"
     gene            complement(1702..1926)
                     /gene="secG"
                     /locus_tag="MJE33_003551"
     CDS             complement(1702..1926)
                     /gene="secG"
                     /locus_tag="MJE33_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBY9649457.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
     gene            complement(2497..3789)
                     /gene="eno"
                     /locus_tag="MJE33_003552"
     CDS             complement(2497..3789)
                     /gene="eno"
                     /locus_tag="MJE33_003552"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBY9649458.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTEKLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
BASE COUNT         1420 a          659 c          451 g         1570 t
ORIGIN      
        1 aaaaaataga acatgtggtc attactaata ataatataga aaattatatc aatttttata
       61 aaaaatttgg tttttcatct aaagaaatga gcaaaaatta taaacttaat gctagaaatg
      121 ttaagataaa tcttaacata aaaggttata aatttttacc ttatgttaaa aattcaaaag
      181 caagtagaga taacttatgt tttaaaataa acacaaatat tgatgagcca aaaaaatact
      241 tagaacaaaa tgagattcaa attgagcgag gaattgtaga atgtacaggt gtctttggta
      301 atataaaatc tatttatttg aaaaaaacta atggaaattt aattgaatta tcaaatcata
      361 gaatcacgga gagtaaacct tggaaaattt aatcctatca ataaatgttg ttcttccttt
      421 gtttctcaca atgtcacttg gatatgttct aaaaaaatta ggtctatttg atactcatac
      481 tttaaataaa atgaataatg tctgcttcaa gtctttcttg ccattattat tattttttaa
      541 tatatacaat acagatttag atggttctct taattttaag cttatggctg tagctgttat
      601 aattattttt tcaatcttct taatcttagt ctttattatt cctaaaattg aaaaagataa
      661 taagacaagg ggtgcattaa tacaaggtgt atttcgtagc aatttcgtta tatttggtat
      721 tcctgtgaca atttcactat ttggcgataa gagtattgga gtcacatctt tattaattgc
      781 agttattgta cctatgttta atgtattatc tgttgtaatt cttgaaattt ttagaggtgg
      841 caaagtaaat ataaaacata tgattaaaag cgtcttaaca aatccactca taatcgcctc
      901 ttttatagga ttgacaatgc taattttaaa tattaatata cctacatttt tacacaaaac
      961 tattggtgat atttctaaaa tagctactcc tttatcactt ataattctag gcggctcttt
     1021 tagtttttct gctataaagg ggcatataaa ccccattata ttgggcgtat gtggaaaact
     1081 tatacttgta cctgctatat ttttaccaat tagcattttt cttggattta gaaatgtaga
     1141 attagcttca cttatgataa tgttatctgc tccaactgca gtttcttcat ttacaatggc
     1201 acaacagatg gattctgaca gtgaattagc tggacaaatt gtagtattta cttctggatt
     1261 ttctataatt acagtatttt taataatatt tattttaaag cagaataatc ttatttaatg
     1321 atatttgata acacaaattg gtattatgag aaacatatat aaaattgatt taactgcgta
     1381 aaaacatatt attatactat ttttactata taatataact ttgtgtatat tatctcatta
     1441 aacatggtga aaatagtgtt gctatatatt ttttaatttg aaaaaaatat ataaatacaa
     1501 acaatatata aatataaata ataaacgcca acaaatagtc aatctataaa ataaaaactg
     1561 gactaatcat ataaaatcat tagtccagct tttattttac gtacttttta tgttgatgta
     1621 cttttattaa aaactaaatt taatttttaa atgtctagca aatgcactaa ttaaataatg
     1681 gccagttatt caacaatatc actacttctt aactataagt aatgccaatg catttataaa
     1741 aaataatact gctgttatct ttgtaactct agcaagaaat gcttgttttc cttttggttt
     1801 aaagtaagct tggcttcctt ctccaccaaa ttctgaaggt aaagcattct tagaatcttg
     1861 aggcattatg ctaattacta gtaaaatccc tagtactact tgcacaccca ttaaaatgtt
     1921 gctcattctt gcccctccta tctgtttatt acttaaattt cattactctt tagtgtatca
     1981 caataatata gccatttcaa gactattatg ctataaataa catacaaaaa aatatattgt
     2041 tactataata tattactata ttctcaatat aaaaatgaat aaaacttaga agagaaaact
     2101 atttaaatta atacataaat taacacttaa aataatctac tctcattgta tatattataa
     2161 atagccacca aaataaaaca atcattttaa aatattgata taacttttaa tttcaacata
     2221 atttaaaata aataatccac atttagcaat aatacttcaa tgacaattat gattaaatca
     2281 aaatatgtct tcacttttat tttttaataa aaacctccta tgcaattaga taaaaatgag
     2341 atagaaacat ttattcatat tttttctgaa tctatgaata aaattaagta ataaaatata
     2401 aaaaaggata agtgaattga aaaatcaatt aacacttatc tcttttatat cttatattct
     2461 ctcttcatta atctttttta gtaaccattt taataactat ttttttaagt tatagaaaga
     2521 ttttaatcca caatatctag cttgctctcc aaccatttct tctattctta ataattggtt
     2581 gtacttagca actctatcag ttcttgatgg agcaccagtt tttatttgac cagcatttac
     2641 tgcaacagct aaatctgcta tagtagaatc ttcagtttct cctgatctgt gagatataac
     2701 agcagtatat cctgctctct tagccatttc tatagcatct aaagtttcag ttattgtacc
     2761 tatttgatta actttaacta atatagagtt agctactcca ttttcaattc ctttttctaa
     2821 tctctctgtg ttagtaacaa ataagtcgtc accaactaat tgtaatttat tacctaattt
     2881 ttcagttaat aatttccatc catcccaatc ttcttcgtct aaaccatctt ctatagttat
     2941 tattgggaag ttgtttgacc aatcttcata taaagcaacc atttcagcag cagttaactc
     3001 ttttccttct cctgctaaaa cgtatttttt agtttctttg ttgtacattt ctgtagcagc
     3061 aacgtcaaga cctaacataa catcttcacc tggcttatat ccagcttttg ttatagcttc
     3121 aactattaat tctaaagctt ctctgtttga acctaagttt ggagcaaatc caccttcgtc
     3181 acctacacca caagctaatc ctttttctcc taaaactttc tttaatgaat ggaatacttc
     3241 tgctcccatt cttaaacctt ctttgaaaca acaagctcct actggtaata tcataaattc
     3301 ttgaacatca acgttgttat cagcatgctc tccaccgttt aatatgttca tcattggaac
     3361 aggtaattgt ttagcattta ctccacctaa gtattggaat aatggtaaac ctatttcatc
     3421 agcagctgct ctagctactg ccattgaaac acctaatata gcattcgctc ctaatttacc
     3481 tttgtttgga gttccatcta attctatcat gattgcatct atagctggtt ggtcaaatgc
     3541 atccattcct tctatttcag gtgctattat ttcatttacg ttagcaacag ctgtttctac
     3601 accttttcct aagtatcttc ccttatctcc atctcttaat tcaacagctt caaaagctcc
     3661 tgtagatgct cctgatggaa ctattgctct tcccattgct ccatcttcta atactacttc
     3721 aacttctaca gttggatttc ctcttgagtc taatacttct ctagcatata ctaattcaat
     3781 aactgacatt tgtatatctc ccttcgattt tctattagtt aaattttcat ttactgtaaa
     3841 catccgtcta ctattttaat ctactttcta tattatagta cataaagttg cttttgaaaa
     3901 ttaggtacaa atttatttga tgggtaaatt tgtaaaagtt cctattttta ttttattttt
     3961 tataatattt taatttatat aatataaaaa aacttgtcta aaatccaaaa ccaatcttaa
     4021 atacacaaat attataacta gatttaaaac attaaacata aaatgtacaa ctaatgcttc
     4081 taaaattttt gcttatataa
//
LOCUS       DAGSRM010000165         4043 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.171, whole genome shotgun sequence.
ACCESSION   DAGSRM010000165 DAGSRM010000000
VERSION     DAGSRM010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.171"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(222..1472)
                     /locus_tag="MJE33_003553"
     CDS             complement(222..1472)
                     /locus_tag="MJE33_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HBY9649459.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLDEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRVIDDI
                     INDFMDNNEPLYDFITDDKIEHTVWKIDNDDIIDNLVDKFEKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLGDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1504..2445)
                     /locus_tag="MJE33_003554"
     CDS             complement(1504..2445)
                     /locus_tag="MJE33_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HBY9649460.1"
                     /translation="MYNILVTDGIEKEAARKLRELDFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLENADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
     gene            complement(2685..3761)
                     /locus_tag="MJE33_003555"
     CDS             complement(2685..3761)
                     /locus_tag="MJE33_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBY9649461.1"
                     /translation="MSKKLFIPGPIDVKEEVLQKMATPMIGHRGKDASMLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATTPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYEDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDILGFNK"
BASE COUNT         1218 a          733 c          428 g         1664 t
ORIGIN      
        1 cacatttaaa accttataaa tatacaaata caattatatg aattttcatc ctcaattata
       61 gcatagaata tgactatcta tatcctaaaa ttatccattt ctaaaaaaaa ggtaactatt
      121 attaagaata tcaacttata gaatttatat tccacatacg attttcaatc aaaaaaacaa
      181 gggctaaaaa gtgaattgtg cactttttag ccctttatta attattcatt tatttcatgt
      241 aagaatagac cacatcttac tttaggttca aaccatgttg acttagctgg cattatctta
      301 tttgcatctg ctactcttat taaatcttca atactagtag gatacattgc aaatgctact
      361 ttcatatctt cattaactct tttttctaat tcttcaagac ctctaattcc accgataaaa
      421 tcaattcttt tatctactct aggattatca attcctaaaa tagcatcaat aacatagtct
      481 tgtaatatag aaatgtctaa tgagtcaaca gggtcttcta tttctaataa atttttattt
      541 gctttcattt tataccactt atcacctagg tacattccga aagttccttt tttgtcaggt
      601 ttatattttt cttttccttc tatttcttct agttcaaaat tttcttttat tttatttata
      661 aattcttcct cgcttaaacc atttaagtct ttgataaccc tattataatc caaaaccatt
      721 aaattctcat ctggtgcaat cattgctatg aagtaattaa attcttcttt tccagtataa
      781 tttggatttt tagctctcat ttctttggct acattttctg ctgaagctgt cctatgatga
      841 ccatctgcaa tgtatagata atctaatttt tcaaatttat ctactaggtt atctattata
      901 tcatcattat caatcttcca tactgtatgc tctatcttgt catctgttat gaaatcatat
      961 aaaggttcat tattatccat aaaatcattg attatatcat ctatcaccct ttgatgtttg
     1021 taagttacaa gtatagttcc cgtattagca tcacagtatt ttatgtgttt agttctatca
     1081 atttcctttt ctggtcgagt gtactcatgt ttttttattg ttccattcaa gctttcatca
     1141 actgatatac atgctactat accaacttga gctctaccat ccatgatttg tttatatata
     1201 tatatacact ctttgtcatc ttttactaga atttcttgtt ttcgaaactc atctaaattt
     1261 tctcgcgctt ttaaataaac tttttcatca tataaatcta tattttcatc taaatcaatt
     1321 tctgatttat ctatatgcaa aaatgaatat ggattccctt ctgcaattct tctagcttct
     1381 ttactattca taacatcgta aggaagtgct gctactttat ctactatata tttatttggt
     1441 ctaatcgcct taaatggttt aatcgttgcc attactaatt tcctcctaat tatacaaaat
     1501 attttataat gctaccccta ttagattgtt gaagtcaaga aagaaatctt gaatcacatt
     1561 tactatttcg tctcctattc tttcttgagc ttctactgta gatgccccaa tatgaggtgt
     1621 aggagaaact cttggatgat ttactaagtc taaatttaat gtaggttctt gttcataaac
     1681 atctatagca gctgctgata atttaccttc atctaatgct tttagtaaat ctttttcatc
     1741 aactaaaccg cctcttgcac agtttattaa ataagcccct ttcttcatct tatttatttc
     1801 tttttcagtt attactgccc ccttattctt atcaaatggt atgtgtaaag ataagaaatc
     1861 tgcattttct aaaacttctt ccatatcaca gaatttataa ttgttaaagc cttgcgcctc
     1921 acccattatg tcagtatata taacattcat acctaataat tctgctctct ttgcaacttc
     1981 ttttgcaatt cttccaaaac ctataagtcc aagtgtcttt ccattaattt ctgtaccttt
     2041 atactttttc ttctcccatt ttccatctct catagttaca tttgcagtat ttatgaatct
     2101 tgcaagaaca aataattgac ctattgtaag ctctgcaaca gatatactgc ttgcatttgg
     2161 agtattcata acttttatgc cattagcttg agcatattta acatctatat tatccagacc
     2221 tactccacct cttactatca acttcaactt tttaccctct aatgctttat ctattacatc
     2281 tttagtaact ttagtagctg aacgtactac aagtacatca acatctttaa gtttatctcc
     2341 taaaacatct ttttcataaa actgctctat cacattaaaa tctaactctc tcaattttct
     2401 cgctgcttct ttctctattc catcagtaac taatatatta tacataacaa actcctccaa
     2461 tttttattca cttaattttt ccaattttta ttttttaaag tattttcatc aataaaaaac
     2521 tttacttaat tcaactttct gtttatataa ttttcaaaat atttttttat taaagtcatg
     2581 tcaaattttt ctattgtttg ttaaaaattt aatatgtaat ttttataaaa tattatttat
     2641 tatatattct aaatttatct taaaacatac atatttttac aatattattt attaaaacct
     2701 aatatatcat caatattatc taataattct tgtacatctt cgacagtata gtcacccata
     2761 tgtgaaattc taaaagtttt ttcttttaaa tctccatagc catttgctat ttggaatcct
     2821 cgttcttgta attttgaatt taaatttgat acactaatac cttgagtatt ttctataaca
     2881 gtaagtgtat tggataagtg attttcattg gtaaaaattt ggaagtgttt tttagcccag
     2941 tttctaacta agtttgccat gtcttcatgt ctgttgaatc tgttatctag gccttcatct
     3001 aatatattat ctaattggaa atctaaagcg aacatgtgag aaagagatgg tgttgaaggg
     3061 tattggtagt ttttcttgat taagtattca tacattgcta acaaatcaag atatactcct
     3121 ctaaaaggta cttgcttagc tctatcgatt gctttttgag aaaatgtgca aatagacatt
     3181 cctggtggta atcccagtgc tttttgtact gatgttatac aaatatctat tccaatctta
     3241 tcaacctcga cttttattcc accggctgaa cttacagtat ctacacagta gattacatct
     3301 tcatactttt taacaacttc tccaatttct tctattggat ttcttattcc agttgaagtt
     3361 tcattgtgag ttacagttat taaatcgtat tctccggtag aaagcacctt atcaaccatt
     3421 tctggcgttg ttgcctctcc taattcaact ttaaatatat ccgctgggac attattagcc
     3481 actgccattt tgtaccatct atcaccaaag gatccacagg aaaatactgc tgcctttttg
     3541 gatgtacagg aacgaatgga gccttccatt aaaccagttc cggatgacgt ggataaaagt
     3601 atcgtgttgt ttgtatagaa tagtttttgc atcttttcac ttatactctt ttggagcata
     3661 gatgcatctt ttcctctatg tcctatcatt ggtgtagcca tcttttgcaa gacttcttct
     3721 ttaacatcaa taggaccagg aataaataat ttcttactca ttataattcc ccccttttta
     3781 gtttgtaata tggatagcgt ttttactgtc cttattttta ataactataa tatcaagctg
     3841 aattaactat gtcaacataa aatttatttt ttatatataa tttattttat atattttatg
     3901 aaaacattca ttttctacct tttcataagt ctgaattttc tgaattattc tctcaaaaaa
     3961 aatatacttt ttatctatat aaattaattt ttttagtatg taaatttgac gctattacga
     4021 taaatctttt tagtataact aat
//
LOCUS       DAGSRM010000166         4040 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.172, whole genome shotgun sequence.
ACCESSION   DAGSRM010000166 DAGSRM010000000
VERSION     DAGSRM010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.172"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(259..477)
                     /locus_tag="MJE33_003556"
     CDS             complement(259..477)
                     /locus_tag="MJE33_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY9649462.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFIEENNI"
     gene            complement(478..885)
                     /locus_tag="MJE33_003557"
     CDS             complement(478..885)
                     /locus_tag="MJE33_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649463.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPII
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKVLFFSVAIVSIFLVTLDINVS
                     NLNIGMSLLVILFIQSLLKLILFIYFDRKGIYN"
     gene            complement(971..1459)
                     /locus_tag="MJE33_003558"
     CDS             complement(971..1459)
                     /locus_tag="MJE33_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HBY9649464.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSFILEDFDIPYKWMYSQMT
                     NRIYEFDDSMIWVWTKRPDLRCSGYLEKGKKGVLLEIEISEDNVLLSEFDLWHLPLMD
                     ITVELYEDEQIEKKVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLSNVKLIKEFV
                     AK"
     gene            complement(1664..2761)
                     /locus_tag="MJE33_003559"
     CDS             complement(1664..2761)
                     /locus_tag="MJE33_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBY9649465.1"
                     /translation="MKEKLEEKSKIIKTSKEYKRTIIEEKDREWTKKLNDILSRGDFS
                     NEKLDKIKDLIPKEILTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNDYDLLANFIND
                     ILKDAPYANRNIKQFTQIKRIIKVETDPTINYIGEKRPRLDILAEDEERNHINIEMQR
                     ALEEDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIFSSKTALYFIELPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIK
                     RALDRLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGIEKGIAKGKREEKFNIII
                     KLKNKGYNLSEICDIIDDLNKSEVEKVYNQN"
     gene            complement(3134..3304)
                     /locus_tag="MJE33_003560"
     CDS             complement(3134..3304)
                     /locus_tag="MJE33_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649466.1"
                     /translation="MDSNNNTIKSTVKKGISFGSCLAMIISYTAWKSIPWAIFHGLMS
                     WIYVLYYWVKYA"
     gene            complement(3462..3905)
                     /locus_tag="MJE33_003561"
     CDS             complement(3462..3905)
                     /locus_tag="MJE33_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBY9649467.1"
                     /translation="MIEIKTIEINKKSQIDLLMLADPCEEMIDKYLNKGTMYALYDNK
                     ELTCIAVVNEISKEICELKNIATYEHFQNMGYASKMIYHLLDVYSKKYSSMVVGTSES
                     GVPFYEKFGFVYSHKIKNFFVDNYPEPIFEGELQCVDMLYLSYNF"
BASE COUNT         1372 a          663 c          361 g         1644 t
ORIGIN      
        1 cctataatta attcattctt taatccatta gcttggtttt tcatctataa gtcatttatt
       61 ctctaatcta taagcttggt ttttacctat aaatcattta ttctctaatc tataagcttt
      121 ctttttcatc tataaatcat tcattcttta atccattagc ttggtttttc atctataagt
      181 catttattct ctaatctata agcttggttt tttacctata attaattcat tctctaatgc
      241 ataagtaata catgaaattt aaatattatt ttcttcaata aattcaaata actcttctac
      301 agttgtatca aataccttag caatatccat tgccagtttt aaagaaggat tgtagcgacc
      361 attttctaaa tgtacaatag tttcacgtct tactcctact gactttgcaa gttcaccttg
      421 tgtcataagg ttcttttctc tataggattt aatttttgtc tttagtaata ctgacaccta
      481 attgtatatc ccctttctgt caaaatatat aaatagaatt aacttcaaca aggattgaat
      541 aaacaaaatg actaataaag acattcctat atttaaattt gacacattta tgtccaaagt
      601 tacaagaaat atagatacta tagctacaga aaaaaacaat acttttattg caatatcatt
      661 aactcttgat agtatacatt ttgatgcctc atcaattaaa tcatcatact ttttattata
      721 gacccactga gctcccatag ctaataccca aataattata ggaagatatt caatactact
      781 tcctttaaaa aaatacttat ttactaatgt agctccaata caataagaaa ttcccaatat
      841 ttctgaaata agtgttgatt taataccagt ttgttgttta gacatataaa atccccctta
      901 aaaagttatg tatttataac acctcttgtt ataaatatat aacttttttg aataaatttc
      961 aataaaaata ttattttgca acaaattctt ttattaattt tacattagat agaggcactt
     1021 tagatgtcac tccttgcaat gtcaaatctt ctatattaac ttcatttaat tttgaacaca
     1081 gttctaagtc aaatattctt tcccaactta ccttcttttc aatttgttca tcttcataaa
     1141 gctctacagt aatatccatt aaaggtaaat gccataaatc aaattctgat aataacacat
     1201 tgtcttcact tatttctatt tcaagtaaaa cacctttttt tcctttttct aaataaccac
     1261 tacatcttaa atcaggtctc ttcgtccaaa cccatatcat actatcatca aattcatata
     1321 ttctatttgt catttgacta tacatccatt tgtatggaat atcaaagtct tctaatataa
     1381 aagaataatc atttgataga tatttattgt ttttattgtt attccaaaat tctaaagtct
     1441 gtatagtata aaatttcata tataaatcaa cccctttgtt tttctataag aaggagatgg
     1501 ttcaaacttt tattttgaat tatctccttt atttatacac ctctttgtac ctattattta
     1561 tatatctctt tatatctacc atatttaaat aacaaagttt tatattaatt taaatttaaa
     1621 agaggaagct ttgtatttat ctagtgccat ctcaatcata gtattagttt tgattataga
     1681 ctttttcaac ctctgattta ttcaaatcat ctataatatc gcatatttca ctcaaattgt
     1741 atcctttatt ttttaatttt atgatgatat taaatttctc ttctcttttc ccttttgcaa
     1801 tacctttttc gataccttct tgaaaaattt cttctttaaa atttctactt ttattcataa
     1861 tatacgcacc tctctttaaa tttaataaat agtcctcatt actacctaat tcttttaatc
     1921 tatcaagtgc tcttttaata acataatccg ttttagatag tattctaata tctatatgag
     1981 aaggattatc aataagttta ccccatatta gaactctgtt tgctatacaa ctggatttac
     2041 gaatttttgg aagttctata aaatataatg cagttttact agaaaaaata tctggatgcc
     2101 cgtccattgt taatctcagg caatttacat aatcttctat gtgattatat tttacatgat
     2161 ttagtatatg tattccaaca gtttttccaa tttctttata ttctttacct tcttctaact
     2221 ttctaccatg tacacgacta agataatatt cagctctttc caaataatcc tcttcaagag
     2281 ctctttgcat ctcaatattt atatggttac gttcttcatc ttcagccaaa atatcaagtc
     2341 taggtctttt ctcaccaatg taattaattg ttgggtcggt ttcaacctta attattcttt
     2401 ttatttgagt gaactgtttg atattcctat tggcatatgg agcatctttg aggatatcat
     2461 ttataaaatt tgcaagtaaa tcgtaatcat tatttaaagt aaacaatgat ttaaatacct
     2521 catcatactc aggcttagca tacccatctt cagttacaac ttctccaaca ttttcactag
     2581 tcaatatttc ttttggaatt aaatccttga ttttatctaa tttttcatta gaaaaatcac
     2641 ctctactaag tatgtcattt aattttttgg tccattctct gtccttttct tcaattatag
     2701 ttcgtttata ctcttttgat gttttaataa tcttagattt ttcttctaat ttttctttca
     2761 tatcaacacc tcttttttat tattacccat ttcttcatac caaagtctga acttctaagt
     2821 aaaatttgca ttattgaaat aagaacctta taaacttata tatcattact aaagttaaga
     2881 ctttaaagag aagcacttag attacaatag attttagata gctttctttt taaaactatt
     2941 gttgataaat gaatgaatat aagggtatgt attaaaatag agatactcat ctttttcatt
     3001 gtttaaataa tgaaaatata ttttatttaa tacaaaaaag agtgccttat aaactaaaag
     3061 ttcacttcga gacactccct tacattttgt ttcttaaact ttacaatatt acatacttat
     3121 ttattaattt aatctatgca tacttaaccc aataataaag tacatatatc caactcatta
     3181 agccatgaaa aatagcccat ggaatagatt tccatgcagt ataagaaata atcattgcta
     3241 aacaagaacc aaaagaaata ccctttttaa cagttgattt tatagtatta ttatttgaat
     3301 ccataattaa tcctccaaaa ttcaaaataa acaagttatt atatatctta tttgaatata
     3361 atacaatata acagcttcta aataaattat actcttatta tacataattt ataaattata
     3421 attccgataa ttatatgaat taattaatta tattaaccta tctaaaagtt atatgataga
     3481 tacaacatgt caacacattg taattctcct tcaaagattg gttctggata attatctaca
     3541 aaaaagtttt taattttatg tgaataaaca aatccaaact tctcataaaa aggaactccg
     3601 ctttcagatg taccaacaac cattgatgaa tattttttac tataaacatc taaaagatga
     3661 taaatcatct ttgaggcata acccatattt tgaaaatgct catatgtagc aatgtttttt
     3721 aattcacata tttctttgga aatttcattt acaactgcta tacaagtaag ttctttatta
     3781 tcatacaatg cgtacatagt tcctttattg agatatttat caatcatttc ttcacatggg
     3841 tcggctaaca ttaaaaggtc aatttgactt tttttattta tttctatagt ttttatttct
     3901 atcataagtt ctccaccctc actttattca aaccttaatt taacaaactt cttacagtac
     3961 ttatttagtt cattttgata tatattttta ctttttaaaa tatagtgata attccctaga
     4021 atattaatac taataataaa
//
LOCUS       DAGSRM010000167         3908 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.173, whole genome shotgun sequence.
ACCESSION   DAGSRM010000167 DAGSRM010000000
VERSION     DAGSRM010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.173"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(158..1981)
                     /locus_tag="MJE33_003562"
     CDS             complement(158..1981)
                     /locus_tag="MJE33_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9649468.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLLIIDSIISTVIPYTIGKVVDIIDIDTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVKEMRSNLFLKFKNLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSVILIPIVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMISNINVVKSFNYERKSVNDFKEVNDRLLNISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGILASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEQEEQEVHNMFKSEINDNKDGNLNSHFVKGEIELKDVSFSYDSKKKILDNINLKI
                     KSGTSNAIIGETGSGKTTIINLITNFYHIDEGSIFLDGKDIYSINRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANAHKFIVKMREGYSTVLSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVESGNHKELIKLKGHYYRNILLTQGK"
     gene            complement(1994..3769)
                     /locus_tag="MJE33_003563"
     CDS             complement(1994..3769)
                     /locus_tag="MJE33_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9649469.1"
                     /translation="MRYFKKYIKKYIVLFLTAVFFLTLEAFCDLAQPTIMAKIIDIGV
                     SNKNLDYILSTGAIMIGITFLGAIFAIIRSVISARVSQNFSYDLRQDVFEKINTYSLK
                     QIDKFGRASLITRITNDINQMQQFVNGMMRVFIKSPIMCIGSLIIAIKLNLKMSIIIL
                     IAVLIIFIIILLNMKIGYRLFKNVQESTDKVNSKLRQFLSGIKVVKLFCRYDYENQSF
                     EELNDELFKNSRKATTMMSFFRPTITIIINVSIIIILLIGRHLIYSGEIKIGIIVAYV
                     NYMTRILTSLIMISHVFNVFVRAKASYERISEVLVDENEEVIEREESLDVGEKDLKFV
                     NCDIIFENVDFSYNKNTDEKILKNVSFKIEEGQNIGVIGQTGSGKTSLVNLIQKLYII
                     DKGDIKIGGRSIYSISEESLRARIGVVFQNSSVFGKSILENVVMGNRDICKEDVVKSC
                     EIANANQFITKLENTYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDCFSAMD
                     INTETIIRKNIKEYISNLREKESKKCTVITISQKISSIIDCDKILVLDEGEVVGFATH
                     EDLLVSSPIYKKIFALQNLVSEDVN"
BASE COUNT         1300 a          657 c          338 g         1613 t
ORIGIN      
        1 catgtatata tccacatact gtacaaacaa atttcttcat aattactttc ctcctaaatt
       61 ttatatatca aattaaattt tttatttatt aagttagtct atatataccc gcaccatata
      121 cctttaaaca atttttttaa aagttttttc taaatactta ttttccctgt gtcaataata
      181 tgtttctata ataatgtccc tttaatttta tcaattcttt atgatttcct gattccacaa
      241 ttctaccatt ttctaaaact attatattat cacaatttga tatggtactt aatctatgag
      301 ctataattat acttgtcttt ccataagtta aattatttat tgcttcttga attttagctt
      361 ctgttgctat atctatgtta cttgtagcct catccaaaat aagtattgga gcatttttta
      421 atactgctct agcaatactt attaattgtt tttgaccttc acttaaatct tcacccctat
      481 ccgataatac agtactatat ccttcacgca ttttaactat aaatttatgt gcatttgcta
      541 ttttactagc atatataact tcttcatcag tagcctccag atttccatac cttatatttt
      601 ccataactgt atcattgaat atataacctt cttgtggaac tactccaaaa caagttctca
      661 aatagtttct gttaatagag tatatatcct taccatctaa aaatatactt ccttcatcta
      721 tatgataaaa atttgtaatt agatttataa ttgttgtttt tccacttccc gtctctccta
      781 tgatggcatt tgatgtgcca gatttgattt ttaggtttat attatctaaa attttctttt
      841 ttgaatcata agaaaaactt acatctttaa gctcaatttc tccctttaca aaatgagagt
      901 ttaaattacc atctttatta tcgttaatct cacttttaaa catgttatgc acttcttgct
      961 cttcttgttc atctataatt tcaaaaactc tttcacatcc agctatggct gaaagtaatg
     1021 tattaaatag atttgctaat tcatttaaag gtcttgtaaa ttgtcttaca taacttaaaa
     1081 aactagctat aacccctatg gttataatat ctttagatgc aagaatgcct cctataatac
     1141 atatagttgc aaatcctata ttactaatca cattcataat aggcattaaa agagaagtgt
     1201 aaatttgcga tttaagggaa atatttagaa gtctatcatt gacttcttta aaatcattta
     1261 cagattttct ttcataatta aaagatttta ctacatttat gtttgatatc atttcttcaa
     1321 tatgtgcatt tagttttccc aactcaactt gttgttgctt aaaaaaaact ctagttctct
     1381 ttgcaataaa attactcaca aacattacta tcggtattaa tattacactg caaaaggtta
     1441 aaattgggct taatacaaac atcataataa aagtcatcaa tatgtttaag attcctgtaa
     1501 ttaactgtac taatgaattt gatattcctg tactgatatt atcaacatca tttgtcactc
     1561 tactcataat atctccagta gaacttttat caaaaaaggt tattggtaga tttttaaatt
     1621 ttaaaaataa attacttctc atttctttaa ctatactttg agataatcta gctgtacata
     1681 tccccttaga cagtttaact gaaaactcaa ttatataaaa tgaaagtaaa ataattattc
     1741 cttttaagaa aatggatttt gaataagtat cgatatctat tatgtcaact acctttccaa
     1801 tagtatatgg tattacagtt gatataattg aatcaattat taatagcaaa aaaataaatc
     1861 tagtaatctt cttatctttt ttaaagtaca taaaaattct tttaaacgtt tttttaaaat
     1921 ctttaggctt ttcttttttc tttaatctac tgaccttctg atttccagta cttaaattca
     1981 atatacttcc tccttaattt acatcttcac ttactaaatt ttgcaacgca aaaatttttt
     2041 tataaattgg agaactaacc agtaaatctt catgtgtagc aaatccaacc acctcaccct
     2101 catccaagac taaaatctta tcacagtcaa ttatagaact aatcttttgt gaaatagtta
     2161 ttacagtaca tttcttactt tccttttctc ttaaattact tatatactct tttatattct
     2221 ttcttataat tgtctctgta ttaatatcca ttgcactaaa acaatcatca agaattaata
     2281 tttgtggttt tttggctata gctctagcta tagtaattct ttgtctctgt cctcctgata
     2341 aatttttacc cctttggaat aaaacttcat cataagtatt ttcgagtttt gttataaatt
     2401 gatttgcatt tgctatttca catgatttta caacatcttc tttgcatata tctctatttc
     2461 ccataactac attctccaat atactttttc caaacacact actattttga aatactacac
     2521 ctattcttgc ccttaaactt tcttcagaaa tactgtatat actacgtcct ccaatcttaa
     2581 tatcaccttt atcaattata tataattttt gaattaaatt tactaaagaa gtctttcctg
     2641 aaccagtttg accaatcaca ccaatatttt gaccttcttc tattttaaag gatacgtttt
     2701 ttaatatttt ttcatctgta tttttgttat acgaaaaatc tacattttca aaaattatat
     2761 cacaatttac aaactttaaa tctttttctc caacatctaa actttcttct ctctcaatta
     2821 cttcttcatt ctcatcaacc aaaacctcac ttattctctc ataagatgct tttgccctaa
     2881 caaaaacatt aaaaacatgt gaaatcatta taagagatgt taatattcta gtcatatagt
     2941 ttacataagc aactataatc ccaattttaa tttctcctga ataaattaaa tgccttccaa
     3001 ttagtaatat tattattatg gaaacattaa tgattattgt aattgtcggt ctaaaaaatg
     3061 acatcatagt tgtagctttt ctactatttt taaaaagttc atcatttaac tcttcaaagc
     3121 tttggttctc ataatcgtat ctacaaaata gtttaactac ttttattcca cttaaaaact
     3181 gtcttaattt tgaatttact ttatctgtac tttcttgtac atttttaaat aatctatacc
     3241 ctatcttcat attaagcaat attataataa atataattaa aactgcaatt agaataatta
     3301 ttgacatctt taaatttaat tttatagcta taattaaact tcctatacac attattggtg
     3361 actttataaa aactctcatc attccattaa cgaattgttg catttgattg atatcattag
     3421 taattcttgt tattaatgaa gctctaccaa atttatctat ttgttttaat gagtatgtat
     3481 ttatcttttc aaatacatcc tgtcttaaat cataagaaaa attctgagaa actcttgccg
     3541 agattacact tcttattatt gcaaatattg cacctaaaaa agttatacct atcattatag
     3601 ctcctgttga tagtatgtag tctaagttct tatttgaaac ccctatatct attatttttg
     3661 ccattatagt tggttgagct aaatcacaaa aagcttccaa tgttaaaaaa aatactgctg
     3721 ttagaaataa tactatatat ttttttatgt attttttaaa atacctcatt caatcatccc
     3781 ttcatagaat tttctcatta ccaaaagtat atcataatgg ctaatttaat tgattatatc
     3841 aattataaat atttgattta ttattaattt ttataccata tattttataa attatttgtt
     3901 tttatttc
//
LOCUS       DAGSRM010000168         3797 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.174, whole genome shotgun sequence.
ACCESSION   DAGSRM010000168 DAGSRM010000000
VERSION     DAGSRM010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.174"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            316..1692
                     /locus_tag="MJE33_003564"
     CDS             316..1692
                     /locus_tag="MJE33_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBY9649470.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSKGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVRKRRFSKLNVHSKVVLFSTATLIFVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPSTIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            1979..2650
                     /locus_tag="MJE33_003565"
     CDS             1979..2650
                     /locus_tag="MJE33_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBY9649471.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            2839..3636
                     /locus_tag="MJE33_003566"
     CDS             2839..3636
                     /locus_tag="MJE33_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBY9649472.1"
                     /translation="MITNINSKDNEKLKYTRALLKSKNRNKESKFIIEGYRIVMLALE
                     CMANLDYVFINEEFENKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVDAIIALKGCVDIYNPK
                     VIRSTMGSIFDMNIIDASQDETVDMLKSLDFNIVSSYLNTENFYDKIDYGSKVALVIG
                     NEANGINEELVSKSDILVKIPIYGKAESLNAAISSAILMYEIKKYLI"
BASE COUNT         1457 a          411 c          688 g         1241 t
ORIGIN      
        1 aaaatttaac ttcaaaaaag tatatgaaat ttatactggt aaaatgataa ataaatttta
       61 agtacaaaat agtaaaagct atgatagttt tagacctatt atatgtaaaa aatatagatg
      121 tttttggtgc aatttaaatt aaaagatata tataaaaatt taaaatattc caaacagaaa
      181 ttataaaaat tactatcatt gtctttatac aatatttata ctataataga ataataattt
      241 agaggaaagc tctaaaaata aacaaattaa gggtcttaac agtagtgatt ttatgttaag
      301 gaggtataaa gaaagttgaa aacaatagtt agaagactta ttctctacgc agatggaatg
      361 cgtcctactc aaataatggt atctgggttt gctgcaatta ttgtaatcgg agcattgttg
      421 ttaacactcc cgatagcgtc gcagagtgga gagagtatag gcttattaaa tgctctattt
      481 acggctactt cagcggtttg tgttactggt ctagttatgg tagatacagc aacttattgg
      541 agtttatttg gacagatagt tataataaca ttgatacaaa ttggtggtct tggatttatg
      601 accgtagcca caatgttttc acttatggca cgaaaaaaaa tacaactaag agaaagatta
      661 ttaatacaag agtcattaaa tcaagctgat ttatcgggac ttgtaagact tacaagattt
      721 gtattaataa taaccataac aatagaggga ataggtgcac tggttttatc aactgtcttt
      781 attccacaat ttggtttgtc taaagggata tggtatagtg tatttcacgc aatatcagcg
      841 ttttgtaatg ccggatttga cttgatgggc agtgtaagtg gcccatttac atcgttaaat
      901 tcttatgtaa acaactttac agtatcaatg actgtttgcg cacttatagt gcttggaggg
      961 ctaggattcc ctgttgtact tgatattgta agaaaaagaa gattttcaaa attaaatgtg
     1021 cattctaagg ttgtattgtt ttcaactgct acacttattt ttgtgggtgc attatttatt
     1081 tttcttatag aatttaatca aaaagctact atggcggact tgcctttaaa gggaaaagta
     1141 ctatcagcaa tatttcaatc agtaacagca agaactgctg gttttaatac acttgattta
     1201 gctactttac gagaaagtag tgtatttgta atgataattt tgatgtttat tggagcatca
     1261 cctgcatcaa ctggtggggg tataaaaaca acaacactag ctgttttaat cattacagtt
     1321 agaagttttc tatcaggaaa atcagatata gaagcttttg aaagaagatt agctccatca
     1381 actataaaaa aatctcttgg tatatttgta attagtatat cagcagttat ttttggaaca
     1441 ttgattattt caataactca gcctaatttt acattagttc aatcagcttt tgaggttaca
     1501 tcagcacttg ctacagttgg ttcaagttta gctggaactc caaacttaaa tgctttaggt
     1561 aagattataa taataatttt tatgtttatg ggtagagttg gttctcttac tttatttatg
     1621 gctatacttt ctggtggtag aagaaagagt caaccaatca gatatgcaga aggtaaaatt
     1681 atggttggtt aagttaccaa ggagtagtat aaatttataa tggatttaga gtagcactgt
     1741 aaatctgttg ataaaataaa tgagcctaga tgtaattata tgaagaaata aaatcaacta
     1801 acgaacaaaa ttaattaatg aaataaaaaa acagggaaat aaaatacaaa taaaaaggta
     1861 aatcaaaatt aaattaatct gtgtatataa aattatatat tgtgtataag ttttgtatgt
     1921 gtaaacaatt aatttaagag atattatata aattgtgtat tagggaggaa gtttcgagat
     1981 gaagcaatat atagtcattg ggtgtgggag atttggaagt tcagttgcgt ctactatgca
     2041 tcttttagga catcaagtaa tggcaataga caaaaatgaa gattcagttc aaagtatatc
     2101 tgacaaggta acccattcac ttatagtgga tgttactgat gagcaagcgt taaggtcatt
     2161 aggtttaggt aactttgatg tagcagtagt tgcaataggt tctgatataa gggcatctat
     2221 aatggcgact cttatagcca aagaaatggg tgtagagttg ataatatgta aggcaaagga
     2281 tgaattacaa gctaaagttc tttataaaat tggtgcagat agagttgtat ttccagaaag
     2341 agatatggga gtaagagttg cacacaattt agtttcggat aatatattag accatattga
     2401 acttgaccca gagtattcaa ttgttgaaat cgtaactcca aatagttggg ttggcaagac
     2461 acttatagag cttgaattaa gagctagata tgagataact gtacttgcta taaaaacagg
     2521 taaaaatata aatgttacac cttctccaga tgaggaactt acagccggaa gtatcctagt
     2581 tataatcggt caaaatacta gtataacagc gataacatct ggaaataagg ggataattag
     2641 aagaagataa tttactattt aatatatatt taattgcaat gaaagtagat agtatcatat
     2701 aattatgaat agttatatga tactattttt ttaatcgaag gtagtattta tttgtaagat
     2761 tagataaaga gaagttaaat taaaagtaag gaggctgtgc taatataaaa atttataagt
     2821 tattagcatt tagataaaat gattacaaat ataaacagta aggataatga aaagttaaag
     2881 tatacaagag cactattaaa atcaaagaat aggaataaag agtcaaagtt cataatagag
     2941 ggatacagaa tagtaatgct tgcacttgaa tgtatggcaa accttgatta tgtatttatc
     3001 aatgaagaat ttgaaaataa gaaagaacat gtaaaactat tagaagattt ggataaaaaa
     3061 aacacaaaga tatacaagac tactaataaa aactttaaag aattagtgga tacagaaaat
     3121 actcaaggaa taataggtgt agtttcattt aagaaaaaaa aattaagtga aagtataaat
     3181 aaaaaagata aatttgtatt gattttagac agaatacaag acccaggaaa tatggggact
     3241 ataataagga ctgctgattc tgctggagta gatgccataa tagcactaaa gggatgtgtc
     3301 gatatataca acccaaaagt aattaggtct actatgggtt ctatttttga tatgaatata
     3361 attgatgctt cacaagatga aactgtggac atgcttaaat cattggattt taatatagtt
     3421 tcaagttact taaatacaga aaatttttat gacaaaatag attatggctc aaaagtagca
     3481 ttggtgatag gaaacgaagc aaatggaata aatgaagaac ttgtatcaaa gtctgatatt
     3541 ttggttaaga tacctatata tggtaaagcc gagtcgttaa atgctgcgat aagctctgct
     3601 atactgatgt atgaaataaa aaaatactta atttaatgta ttgtaataaa tataatgtca
     3661 tgttataatc ttgaattaaa tagtagagta tcaaaaatag tcaatatata ttaaaaaaat
     3721 aattaaatta attatatatt agatgtatat tatataaaaa ataattgagt tagttatgca
     3781 ttagatgtat attatat
//
LOCUS       DAGSRM010000169         3689 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.175, whole genome shotgun sequence.
ACCESSION   DAGSRM010000169 DAGSRM010000000
VERSION     DAGSRM010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.175"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(516..1835)
                     /locus_tag="MJE33_003567"
     CDS             complement(516..1835)
                     /locus_tag="MJE33_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBY9649473.1"
                     /translation="MLNINVFWIFLPIFSTTIEWIVLKFLLNEFSELRKNRLTLNSSI
                     VIAIMIITMLNTIEFNINIKLFICIFITYVLYKINYVVDKWKSVFISLLYWMLLIGFD
                     SIGLSIVMTLNSIKNMNDLLEDNLLKLELIMISKSLLIVLIPLLKFIRLKTKINKRDC
                     IYLSIPLIANIIGVIVIFEFIFKDKIIDSKESLIILTISTVFLLSNLSLVSIIGRIIK
                     DNSLKIEYEITKKRMNMQYKYYLNLQEYQLKIRKLYHDMNNHIICIQNVYGKNEFADK
                     YIKDINKQIKECNTIFNTQNMILDVILSDKKSICDMNNIYFLVDINFLECNFIEMPDI
                     CSIFSNIIDNAIEACIKIKDTSIQKKIKLRGTVINRLFVIKCENTKINEVVLKNNKVI
                     TSKKDSFLHGIGISSVKSSVEKYNGNVEIYSDKNKFTITIYIPLTRN"
     gene            complement(1829..1957)
                     /locus_tag="MJE33_003568"
     CDS             complement(1829..1957)
                     /locus_tag="MJE33_003568"
                     /inference="COORDINATES: protein motif:HMM:NF016296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649474.1"
                     /translation="MEYIQFICKNTVVINGKDIPVSKYRMANLKKKLTYVLGDVLC"
     gene            complement(2096..2806)
                     /locus_tag="MJE33_003569"
     CDS             complement(2096..2806)
                     /locus_tag="MJE33_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY9649475.1"
                     /translation="MYRIVICEDDITQIAFLRECILKSLEGISSQIELFEFNSGEELL
                     ETNLEGIDIFFLDIKMLQLTGMDVAKIIRETNDTSEIIFITSIVDYIQEGYKVRAYRY
                     LLKPIDFGDLNESILSCISDIIKKRENFMLIENKGIINKILINSIMYIEVRKKVLTIH
                     TKNDTYYTKNSMDKIELELEKYNFFRCHKSYLINLEYIQFICKNTVVINDEDVPVSKY
                     RMADLKKKLTYVLGDVLC"
BASE COUNT         1405 a          518 c          262 g         1504 t
ORIGIN      
        1 taaataatta aatcttctac attaaaaatc atttcttcag aatctatcac aactaagtcc
       61 tcctcaaatt agtaaaatat ttttctttaa tatttattac taatatcaac tttactatct
      121 aattagttat ttaattgttg tttttatgtt cctagttata aagtttatca aatttacatt
      181 ttattttttt atactatata gaacttttgg tcaataatgt taccatacta aggtcgcatt
      241 tgcagataaa aataactcta tttttctaaa tttatttaag atatatcatg caaataatat
      301 aactataaaa cctatcaaca ttttaaattc aaacaaatat aaatcacatt ttttgttcta
      361 ttattttatt gagtactaaa aaggatgtta tcttattagc atagcattca accattttta
      421 tcacctccaa atatcctatg ccttaaagat agcatcttct attttatctt tcaatactac
      481 tgctccaact gggcaattta gagccccaac tagaattagt tcctagttaa tggtatataa
      541 atagtaattg taaacttgtt tttatctgag tatatttcca catttccatt atatttttct
      601 actgaacttt ttacactact tattcctatt ccatgtaaaa aggaatcttt cttacttgta
      661 ataaccttat tattttttaa tacaacttca tttatctttg tattttcaca ttttattaca
      721 aataacctat ttataacagt tcctcttaat ttaatctttt tttgtatact tgtgtcctta
      781 atcttaatac aagcttctat tgcattatct attatatttg aaaatatact acatatatct
      841 ggcatctcta taaaattaca ttctaaaaaa ttaatatcta ctagaaaata tatattattc
      901 atatcacaaa tagatttttt atcacttaaa ataacatcta gaatcatatt ctgagtatta
      961 aatatagtat tacattcttt tatttgctta tttatatctt taatatactt atctgcaaac
     1021 tcatttttcc cataaacatt ctgaatacaa atgatatgat tattcatatc atgatatagt
     1081 tttcttattt tcaactgata ttcctgtaaa tttaaataat atttatattg catattcatt
     1141 cttttttttg taatttcata ttctatcttt aaactattat cttttattat tcttcctatt
     1201 atgcttacta atgaaagatt tgatagtaaa aatacagttg atattgtaag tattattaaa
     1261 ctctctttag aatcgataat cttatcttta aaaataaatt caaatattac tattacacca
     1321 ataatgtttg ctattaatgg aatagataaa tatatacaat ccctcttgtt tatttttgtt
     1381 tttaatctga taaacttaag caatggtatt aatactatta gtaatgattt agatatcatt
     1441 attaactcta acttcaacaa attatcctct aataaatcat tcatattttt tatagaatta
     1501 agtgtcatta ctatacttaa tccaatacta tcaaatccaa ttaataacat ccaatatagc
     1561 aaacttataa atacactttt ccatttatcc actacataat ttattttata caatacatat
     1621 gtaataaata tacatataaa taatttaata tttatattaa actcaattgt attcaacata
     1681 gtaattatca taatagcaat tactatgcta ctatttaggg ttaatctatt ctttcttaac
     1741 tcactaaatt catttaataa aaattttaat acaatccatt ctatcgtcgt agaaaatata
     1801 ggtaaaaaaa tccaaaatac atttatattt aacataatac atctcccaat acatatgtta
     1861 attttttctt taaattagcc attctatatt tactaactgg tatatcttta ccattaataa
     1921 ctacagtatt tttacatata aattgaatat actccaaatt aattaaatag ctcttgtaac
     1981 atttttaaaa atttattctt tcttaaatca ctgaattcat ttgataaaaa ttttaataca
     2041 attcattcta tcgccgtaga aaatataggt aaaaaaatcc aaaatacatt tatatctaac
     2101 ataatacatc tcccaataca tatgttaatt ttttctttaa atcagccatt ctatatttac
     2161 taactggtac atcttcatca ttaataacca cagtattttt acatataaat tgaatatact
     2221 ccaaattaat taaatagctc ttatgacatc taaaaaaatt atatttttct agctctaatt
     2281 ctattttatc catactattt ttagtataat aagtatcatt tttagtatgt atagtcaaaa
     2341 cctttttcct aacctctata tacataatac tattaattaa aattttattg attatacctt
     2401 tattttctat cagcataaaa ttttctcttt tttttataat atccgatata caactcaaaa
     2461 tactttcatt taaatctcca aaatctatag gctttaataa atatctataa gcccttacct
     2521 tatagccttc ctgtatgtaa tcaactattg aagttataaa tattatttca gaagtatcat
     2581 tagtttctct aattatttta gccacatcca tgcctgttag ttgaagcatt ttaatatcta
     2641 aaaaaaatat atcaatacct tctaaatttg tttccaataa ttcttctcct gaattaaact
     2701 caaatagttc aatttgacta gaaataccct ctaaggactt taatatacac tctcttaaaa
     2761 aagctatttg tgtaatatca tcctcacaga taactattct atacataaac acccctcctt
     2821 agacattaga tctgaaaaca aataaaatat caatttataa ataattattt agttatacaa
     2881 ctaacatggt catcaaatac tattattcac ttaaaaaatc accccctatt tttagtatgt
     2941 taacaacaaa ataagaagat atcttagatt tttaaaaaca attaatactt aatgtagttt
     3001 tgtatactat ttataggtaa ttttttatat cattttaaac attaaaatta ttgatgttta
     3061 agaactatta tattgatagt aatatataat taatgatatc aaaaattttt aattagactt
     3121 ctctcttatt caagattttt ctatataaaa tctaagaata aatagaaaac taacatctaa
     3181 ccttttattt tttattttcg atctatttac ttgaatttta acataaatct atcttttagt
     3241 atatattaat catgctgaat ttgtgatgac atgtatacaa aatatcgttt agtattttct
     3301 aataaaaaat attactgaaa ttaaaaatct acaatagcat ttaacaagta aaaacatgtt
     3361 tacacaaata tataatttaa ggtctttaaa atagagtatg tattattcta aaaattagtt
     3421 atagttatca cataaaaata gaaaagaatt ttcaaaagat ttaaagaaca tacatacttc
     3481 aattgcagaa gatgtaatgt taagtgaact agcattcttc gaggaaaaat agactcaaaa
     3541 aattagaaaa tagaatcagg ttttagatga tttaagtatt tattttcaaa aaagaaattt
     3601 gattctaagt taataaagaa atatttaaaa aaatcaaaaa attttcttaa ataagtaata
     3661 ggttttgtaa aaatggaaaa atatcaaaa
//
LOCUS       DAGSRM010000170         3306 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.176, whole genome shotgun sequence.
ACCESSION   DAGSRM010000170 DAGSRM010000000
VERSION     DAGSRM010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.176"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..894)
                     /locus_tag="MJE33_003570"
     CDS             complement(<1..894)
                     /locus_tag="MJE33_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBY9649476.1"
                     /translation="MKYKLFIDFEFNILDDNKNKPKEYNGAELISIGGVLVDNEFNTI
                     DNYYSLVKPKYNKILSNKCKNLTKLNQLDINNAPNLLYVMDDFYKWFCKFDDVTIYNW
                     GDFDITGLLTSFRVYKYTGKCLELFNMMIDIQPFISQHITYNNRILSKQISLLNMKKI
                     FSVEGDIKHNSLSDAVDLMNVCKCFFFNSPKNIDILEELYNKLPALKTPLYYIPYFED
                     ENFELKFDRTPEDIIIYLKQILTILNISKKEIYFKKRSVLVNNSKIINFKNLSSSFKL
                     IKLNEFEEYPDFILKLGDKKSF"
     gene            complement(934..1281)
                     /locus_tag="MJE33_003571"
     CDS             complement(934..1281)
                     /locus_tag="MJE33_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649477.1"
                     /translation="MLNLKEIREEIIKKYIGDSLISELTEELKNILITDLQSNPFKSN
                     IELSIIPANIEIFKKIENILIDSKVNLKMGWERILINYILKDLFELNYEINKSCNEPL
                     MFRVIISIDSYDA"
     gene            complement(1499..2455)
                     /locus_tag="MJE33_003572"
     CDS             complement(1499..2455)
                     /locus_tag="MJE33_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBY9649478.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRVKEGNPVC
                     ESGIFEILDVTDKNRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EIAKELDCKFFTPSSIAAFGENSPKNMTPQDTLQRPNTMYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIEAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPVRQSIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTKDMLEKLSEKGIGK"
     gene            2898..3266
                     /locus_tag="MJE33_003573"
     CDS             2898..3266
                     /locus_tag="MJE33_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HBY9649479.1"
                     /translation="MTRPSIYHSQGYTCAEALIKSYNEEHNTDIPISIGSGMGVGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELLNRVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
BASE COUNT         1194 a          439 c          416 g         1257 t
ORIGIN      
        1 aaaactcttt ttatctccta attttaagat aaaatcagga tattcttcaa attcattgag
       61 ttttattaat ttgaaagaag aagatagatt tttaaagtta ataattttag agttattcac
      121 taatacactt ctttttttaa aataaatttc ttttttactt atatttagta tagttaatat
      181 ttgtttaaga tatattatta tatcttctgg agttctatca aatttgagtt caaaattttc
      241 atcttcaaag tagggaatat aatataaagg tgttttgaga gcaggtaatt tattatataa
      301 ttcttcaaga atatcgatat ttttagggga gttaaagaaa aagcatttac atacattcat
      361 taaatccaca gcatctgaaa gtgagttatg tttaatatct ccttctacag aaaaaatttt
      421 tttcatattc agtaaagata tttgcttaga taagattcta ttattgtatg taatatgttg
      481 agatataaaa ggttgaatgt caatcatcat attaaataac tctaagcatt tacctgtata
      541 tttatacact ctaaatgaag ttagaaggcc agttatatca aaatcacccc aattatatat
      601 tgtaacatca tcaaatttac aaaaccattt ataaaaatca tccataacat ataaaagatt
      661 aggagcatta ttgatatcta gttggttcaa tttggttaaa tttttacatt tatttgaaag
      721 tattttattg tattttggct taactaaaga gtaataatta tctattgtat taaattcgtt
      781 atcaactaaa actccaccaa tagaaattaa ttccgcacca ttatattctt ttggtttatt
      841 tttattatca tctagtatat taaattcaaa gtctataaat aacttgtatt tcataaatct
      901 aaagcacatc ctcaggtaaa ttatttattg agcctaagca tcataagaat caatagatat
      961 aatgactcta aacatcaaag gttcattaca acttttattt atttcataat ttaattcaaa
     1021 taaatctttt aagatataat ttataagtat tctttcccaa cccattttta aattaacttt
     1081 agagtctatt aatatatttt ctatcttttt aaatatttct atatttgctg gtattatact
     1141 gagctcaata ttacttttaa acgggttaga ctgtaaatct gttataagta tattttttaa
     1201 ttcctctgta agttcagaaa ttaaactatc acctatgtat ttttttatga tttcctctct
     1261 aatttctttt aaatttagca atattagtac ctgctactta catagtagct tttcctttcc
     1321 ttttaatact attattcatt tttgaatctt atttagatat attttaagat taaaagtctt
     1381 tagctatata attttttacc catttttaac ttatattaac atattacagg cttatatctc
     1441 caccctgttt acattgagga tggagtatat tgcctatttt gataaaaatg ataggttgct
     1501 atttgcctat tcctttttct gataattttt ctaacatatc tttggtcatt ttatctaaat
     1561 cataactaaa cttaaagttc cattctttta ctgcactact actgtctaga gagttaggcc
     1621 aagaatcagc tatactttgt cttacagggt ctacatcata ttctatcaca aaatcaggaa
     1681 tatatttttt tatagaatca gctatttctt caggagaaaa gctcatagca gtaatattaa
     1741 aagcatttct atggatgagt ttatcagctg gagcttcgat taaatccact attgattgaa
     1801 gtgcatcagg catatacatc atatccattt ttgtaccttc tgctatgtaa gatttatatc
     1861 tcttattttt taatgcctca taatatatat caactgcata atctgtagtt cctccaccag
     1921 gaggcgtaac ataagaaata agacctggaa atctaacacc tctagtgtct actccaaatt
     1981 ttgagtgata ataatcacat agtaattctc cagaaacttt agtcacacca tacatagtat
     2041 taggtctttg taatgtatct tgaggagtca tattttttgg agaattttca ccaaaagcag
     2101 caattgaact aggtgtaaaa aatttacaat ccaattcttt ggctatttca agaccattta
     2161 ttaatccatt catatttaag ttccaagctt ctagaggttt actttcagca actgcagaaa
     2221 gtaaagatgc tagatgaatt atagtatcta catcatattt ttttgcaatt tcaaaaaaac
     2281 gattcttatc tgtaacatct aatatctcaa atataccaga ttcacatact ggattacctt
     2341 cttttactct ccttgaacta gcgattacat tttgttctcc atactcattt ctaagcttta
     2401 ttgttaattc tgaacctatt tgtcctaatg cacctgttat aagtattttt ttcaatttta
     2461 caacctccta agggtttaat ttatgtagta ctacttattt ttattttagc aaatcttttg
     2521 tttaaataca aagaattaaa gataataata tacgacttaa ttgaatgaag tgcagtaagt
     2581 gcgtaaaaat ttaaagctaa tatataatgg tctattgtat aacaataaaa gagtagatta
     2641 aattattata tatagattgt gtgactaagt cacagaaaat agagctaatt taatatataa
     2701 taaaagcatg tcaagtaaag ctttaaatta aagtaacttt atataaaaag taatataaat
     2761 tgataaaatt ataaatttat ttatagtgaa tataggttgg attttataga tacaaaaata
     2821 gtatatatga tatgtagacc attagcatgt ttaaagtagc aaaacatata aaataaaatt
     2881 tgaaaggaag tataaaaatg acaagaccat caatatatca tagccaagga tacacttgtg
     2941 cagaggcatt aataaagtct tataatgagg aacacaacac agatatacca atctcaatag
     3001 gaagtggtat gggggttgga atgaatgttg gaagtgtttg tggagctgta aatgctgctg
     3061 ttgttataat agggtattta aatgggcgaa atagtaattt agatgaaaat atagcaagga
     3121 agtattcaag agagctattg aatagagtaa gaaataaata cagctcagaa atatgtgtat
     3181 ctcttaaaaa aaataaagta acttgtgaag aaataataaa tttcacatat gatgctctaa
     3241 atgatatgct aaaaaatcaa aaataattct aagaaacaag tattttacat tcgatagaat
     3301 acttgt
//
LOCUS       DAGSRM010000171         3249 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.177, whole genome shotgun sequence.
ACCESSION   DAGSRM010000171 DAGSRM010000000
VERSION     DAGSRM010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.177"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     regulatory      131..224
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            307..792
                     /locus_tag="MJE33_003574"
     CDS             307..792
                     /locus_tag="MJE33_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBY9649480.1"
                     /translation="MISELMLKNFRKSSINFKTKIKLNLREKPDINSLKLKSIPEGKI
                     VKLKCVDGIWAEVESNYDKGWLLYKYLERLSNAKNNSNDTIKNRKKNYIGNIRTNGLS
                     LELRNDRTLESKVITTIPDGFKVEVCYSVGKWARVNINKNEKRYSGYVYNQYIEEHLS
                     L"
     gene            1147..1545
                     /locus_tag="MJE33_003575"
     CDS             1147..1545
                     /locus_tag="MJE33_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649481.1"
                     /translation="MNKKKKLIGIAISFLLIISIINPLQVEAEAIIESSVYISSIKEN
                     YTYDNTKFKTIKSKSRHKSRKYNKSKVKLKKRSIRTKKYGYQTINKYKRQNTRRHNIL
                     TKLILSIIVIFLVIVLVVLIFIKRKKKKFY"
     misc_binding    1981..2244
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2395..2946
                     /locus_tag="MJE33_003576"
     CDS             2395..2946
                     /locus_tag="MJE33_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HBY9649482.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNRDDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMNISYNMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLAAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
BASE COUNT         1391 a          286 c          456 g         1116 t
ORIGIN      
        1 ctataaagaa cagtaattga ataaatataa agatattttt tgattattat taattaaaaa
       61 tttaatttat atttaaataa tataaaattt gaggtaatat tattttttaa ttgttcaaat
      121 tatgttatta ttcagatata ggcaatatta gataaatcta atgacgcaaa actataggga
      181 ctaaaatctt tatgtaataa aggtcatgtc agccagttgc caaaaagata ataattcttt
      241 ttgttaaaag tatttttaga gttatatatt ttaataactt aatatttggc aagaggaggg
      301 cttaagatga tatctgaatt aatgttaaaa aattttcgta aatctagcat caattttaag
      361 actaaaatta aattgaattt aagagaaaaa ccagacataa atagtttgaa attaaaaagt
      421 ataccagaag gtaaaattgt taagttaaag tgtgtagatg gaatatgggc agaagttgag
      481 agtaattatg acaaaggttg gttactttat aaatatttag aaagattaag taatgctaaa
      541 aacaatagta atgatacaat aaaaaataga aaaaagaatt acataggaaa tataagaaca
      601 aatggattaa gtttagaact tagaaatgat agaacattag aatctaaagt tataactaca
      661 atccctgatg gttttaaagt tgaagtatgt tatagcgttg gaaaatgggc aagagttaat
      721 atcaataaaa atgaaaaacg atattctgga tatgtatata atcaatacat agaagagcat
      781 ttaagtttat aatacgatac tagcaagctt taaatatatt aataaatcgc aaaaaagaat
      841 cccattaatg ggattctttt ttgtagaaac attataacag aactaaagag aataaattaa
      901 ctaatttatt tttattataa agtaattata aatggtaaat ttaattgaaa gtagatttaa
      961 cttatacttt atacaccaag tatcataaat taatactact ttgaaaaatc ttaaaaaaag
     1021 taaaagttat gataataaaa ataatattaa agataataag atatataaga aacttgaaaa
     1081 atttgtaatt tttagttata tatatattat aatgaactag ttaaaaattt ttatgagggg
     1141 gaaaatatga ataagaagaa aaaattgatt gggatagcta tatcttttct acttataatc
     1201 agcattataa atccattaca agtagaagca gaagctatta ttgagtctag tgtatacatt
     1261 tcttcaataa aagagaatta tacatatgat aatactaaat ttaaaactat taaatctaaa
     1321 agtagacata aaagtaggaa atataataaa agtaaagtaa agttgaaaaa acgaagtata
     1381 agaactaaaa agtatggata tcaaactata aataaatata aaagacaaaa cacaagaaga
     1441 cataatatcc taacaaaatt aatattgtcc ataatagtca tatttttggt tatagtttta
     1501 gtagtgctaa tatttattaa aagaaagaag aaaaaatttt actaaaaata attaaaagaa
     1561 tatcattttt ttaatcattg gtatctattt aataatatga tgtaattaaa atggagtaat
     1621 attctgtttc aaaggaggca tataaataca ggaatgagta ttaaatgatg gttaattaag
     1681 taaatttatt gataaatgta cttaattaac tttgaagaat ttaattcctc atgaatcaaa
     1741 atataaaatt acatcaacag ttattcacaa aattaatata agaagaatta gagtcaaaga
     1801 gcagactatt agaccattaa agtaattatt catagaattt tgtatatgaa aagaaatgta
     1861 tagtataatt tacttttttt ataaaaaata gattacaaaa tatatttttt ataaaaaggt
     1921 attgacattt tttagaaaaa agaatatcat tattttaaca acaaaattca atacaataaa
     1981 ttaaatccta tgacaaggaa gagtagttaa atttaggttc agtatagaga gctgaggatg
     2041 gtggaatctt agcaagaaaa aatttgatga atggccctta gaggaataaa ttgaaatcat
     2101 agttttgaga gtagacttta tcgtcagtcg cacgttatag cgaataaggt atgtaagtac
     2161 ctgataatga gatgtatgaa aattgaattt tatattaatt tttatataat ttaggtggca
     2221 acgcggataa tctccgtcct attagtttag gacggagttt tttattttac tgaaaattta
     2281 aaagatagaa ggtggtgaga aagatgttat gaatttcaaa agctaaagat gatgatatag
     2341 taggttggca aaaaaataaa ttgaaaatta aaaaaatagg aggaagtaca aaaaatgaaa
     2401 acaaatacaa aaaaattaac tttaaatgca atacttttag cgatgggatt attaatacat
     2461 caaatcacac cagctatagg attaccaatg aagccagatg taccactagc aatgttattt
     2521 gtaatattgg ttttaaatag agatgattat aaaacttgtt tgatagcggg tatagtaaca
     2581 ggaatattta cagcacttac atcaagtttt ccgggaggtc aaattcctaa tgtaatagat
     2641 aaaactctta cagcaaatat agtattttta ttaatgaata ttagttataa tatgcctttt
     2701 ataaaaaaat tagctaagaa aacacaagat actgtagtag ttgcaataat aatgccaata
     2761 ggaactcttg taagtggaac tatcttttta ttagcagctc aagctatagt tggtcttcca
     2821 ggggcttcat ttacagcttt attcttagca gtagtattac cagctgtttt aataaatcta
     2881 gttgcaggaa tatttttatt caagattgtt agtctatcaa taagaagagt tagttatcaa
     2941 gcttaaacac gaaataatat tttactttat aaagcattat aataactaaa taacataaga
     3001 ataaagtaga ttataactat tttattctta tgttgtttaa agatttggta ttatatattt
     3061 atttttaata aaatatttat atagtaccaa ttacacttaa tttaacagat aaattatcat
     3121 acctatataa tattcttata tgtatctcat ttgtagttat taaatataag atatatttaa
     3181 ttgacttttc aagataaatt tttatattaa tctcctctga tttctcattt tttgaataca
     3241 ctaaaattt
//
LOCUS       DAGSRM010000172         3155 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.178, whole genome shotgun sequence.
ACCESSION   DAGSRM010000172 DAGSRM010000000
VERSION     DAGSRM010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.178"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            125..919
                     /locus_tag="MJE33_003577"
     CDS             125..919
                     /locus_tag="MJE33_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9649483.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKVKTKKEEFFAVNNVS
                     FKVMEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINISGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEDIEESIENIVKIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGKIIASGTPQSLKEESGVNNLRDVFIKLARKEDI"
     gene            920..2980
                     /locus_tag="MJE33_003578"
     CDS             920..2980
                     /locus_tag="MJE33_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBY9649484.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKDTKNTSNDKGKIRVLDVDNYK
                     KSLEENKIDAYIKMEEKKSSINYQIYINSSQENSSNALKRIETVLDKYKRFTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMAFILVSGGISSQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSIPTVSDGVILYAVGLVLLIYVGSLVQ
                     IKFGMAGIIMTQFMIISLPLLFAYYIKADFKEVFSLKVPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQNMEVAESLNKALFIKDSVLLNLLAIALLPALCEEMFFRGFIFSSF
                     SKSKDKNKSIKLAIICSGVLFGIMHMDFIRIIPTSILGIIFAYSVYKSGSIFVSMLLH
                     FLNNSVAVVLNHYTTGNITNLYKFVEVDFSNLNVLKFALILLISLILIMLGLRVLDRK
                     SLRN"
BASE COUNT         1205 a          322 c          512 g         1116 t
ORIGIN      
        1 gccagataaa cttgtaaatt tttatgaata atccattcta aaacaaaaaa ctgtgtataa
       61 aaaaggttta tatgttatat ttatataaat ataacatatg aacgagaaag gagtggtttg
      121 ataaatgatt gaggtagata aattgtgtaa gtcttttaca agggttgtaa aagatgataa
      181 gaataaaagt tcaataagaa agctaaagaa agtaaaaact aaaaaagaag aattttttgc
      241 tgtaaataat gtttcattta aagttatgga aggtgaaatt gttggaatat taggtccaaa
      301 tggtgcagga aagactacat tacttagaat gttaggtggc atattaacac caacatcagg
      361 aagtataaat atttctggat atgattattc aatagataga aattcagcaa aaaaagaaat
      421 aggttatctc tctggaaata caaaactata tggaaggctt tctccgagag aacttctaac
      481 aacttttgca agtctatatg agatgtcaaa agaagatata gaagaatcaa ttgaaaatat
      541 tgtaaaaata atggatatga gtgaatttat agataataga attgaaaatc tatcaactgg
      601 tcagactcaa agaacatcta tagctcgttg tttaatacat tctccaaaag tatatatttt
      661 tgatgagcca acactaggtc tggatgtttt aagtagtgca tctattatag attttatgaa
      721 gaatgaaaaa ctaaggggaa aaactgttct ttactcaact cattatatgg aagaagcaga
      781 aactttatgt gacaagattt ttatgattca caatgggaaa ataattgcat caggaacacc
      841 acagagttta aaagaggaat ctggagtaaa taatttgaga gatgtattca taaaacttgc
      901 cagaaaggag gacatttaaa tgagaagtaa gattgtaaaa tacatattta aaaaagaaat
      961 gttagacata ttgagagata aaaagacttt atttatgatg attgttgtgc cacttctttt
     1021 atatccttta cttatgttat taatgattca agtaatgaat atgtctgcaa gtagtatgaa
     1081 tagtaaggac attaatatag cttttaataa taaacctaat aaggttttag tatccatgat
     1141 tgaagatgat aatttagaaa aagatactaa aaatacttca aatgataaag gcaaaataag
     1201 agtgttagat gtagataact ataaaaagtc attagaagaa aataaaattg atgcttatat
     1261 aaagatggaa gaaaaaaaat catctataaa ttatcaaata tatataaatt cttctcaaga
     1321 aaattcttct aatgcactaa aaagaattga aactgttttg gataaatata aaagatttac
     1381 catagaaaaa actttatctg aaaaaggatt agatgtacaa gctactttag aaccaataac
     1441 atatagcact gtagatgttg caaagacgga agaagtggca ggatactttt taggtcagat
     1501 acttccattt atattaataa taggagtttt actaggttca atatatccag caatagatgt
     1561 tatggctgga gaaaaggaaa gaggtacatt agaaacatta tttacactgc caatatcaaa
     1621 cttagaactg gttatgggaa aatatatggc agtttctctt agtgcaatag taacagcaat
     1681 tttaaacatt ttatctatgg gactgactat ggcatttatt ttagttagtg gaggtattag
     1741 tagccaattt ggctttggaa actttaatta tggagactta gtcttaccaa ttataactac
     1801 tttaatttgc atatgtcttt tttcaatggt agtttcagca ataagtatgt gtgtatgttc
     1861 attagcaaaa agttttaaag atgctcaaaa ctacataaca cctcttatgt taattattat
     1921 gataccatca tatgtttcta tgataccaaa tatagtatta gatagagtga cagctgtaat
     1981 acctgtagta aatatatctt tacttataaa aagtgtatta tcttttaaaa gcgatttaaa
     2041 tttaatagcc ttagttttag catcaaatgt agcctttgtt atattggcaa tcatactact
     2101 gtctaagatg ttcaattctg aggaaatact ttttggtaat agtaagagtt tctctttctt
     2161 agagaaaaga agtaatataa aaaaagggtc tataccaact gtaagtgatg gtgttatttt
     2221 atatgcagta ggattagttc tattgattta tgttggttct ttagttcaaa ttaaatttgg
     2281 tatggcaggt attataatga cacaattcat gataatttct ttaccgttat tatttgcata
     2341 ctatattaaa gctgatttta aagaggtatt tagcttgaaa gttcccaaat taaagcatgt
     2401 gtttgggagc attgttcttt ggattggtgg atacatactt attatgatta taacacagct
     2461 aattttattc ttatttcctc aaaatatgga agttgctgaa agtttaaata aggctctttt
     2521 tatcaaagat agtgttttac tgaatttact agcaatagct ttattaccag ctttatgtga
     2581 agaaatgttt tttaggggat ttatattttc ttcatttagt aaatcaaaag ataaaaataa
     2641 gtctatcaag ctagctataa tatgcagtgg ggtactattt ggtattatgc atatggattt
     2701 tattagaata attccaacat caatattagg tattatattt gcatatagtg tgtataaatc
     2761 tggttcaata tttgtatcaa tgttattgca ttttttaaat aatagtgtag cagttgtgtt
     2821 aaatcattat acaactggaa atataaccaa cttatataaa tttgttgaag ttgatttttc
     2881 gaacttaaat gtacttaaat ttgccttaat tcttctaata agtcttatat taattatgtt
     2941 aggattaaga gttttagata gaaagtcttt aagaaattaa gtataaagtt attatataga
     3001 ttaaaatatt aataagcact aaagtatatg gatatatctt gtatataaaa atactttagt
     3061 gctattttat atattttaat tatatgaatt atttatcgtt tcatttatgt aatgaaaatg
     3121 ttatataaat gaaacaataa ataattttta tttta
//
LOCUS       DAGSRM010000173         2840 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.179, whole genome shotgun sequence.
ACCESSION   DAGSRM010000173 DAGSRM010000000
VERSION     DAGSRM010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.179"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            381..2192
                     /locus_tag="MJE33_003579"
     CDS             381..2192
                     /locus_tag="MJE33_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBY9649485.1"
                     /translation="MKKKYDFLIYSAIWLVIIILFIFSLYHSIEHIKIISHTGTIRSE
                     TQKVVKQELNNERNDDLIKRLDNILIKLRTGNGENGFQRCDNKEFQQKLNQMDSMWES
                     MKKEIIKVRNGASGDKLYKLSEEYSVLSNQIVFISEKHSNAKLYSFATALFIYLIFST
                     ISLLIWEYYNKKRFKRIFYTDNLTKIKNQVAFENRAIEILHNASNKEYVLLNIDIDNF
                     KYINDTHGYEYGDKVLIIVAAALSKTFNIKETCARIGSDNFVILAKYRDSLLEDIREM
                     LTNAIISELDMNVTQTISYCIGAYLVEIDNLGYKSINSIMDKANIAHKVSKTRGISST
                     VWYNENLLKQLQMENSIYNYMYKAIENEEFHMYLQPKFQISSLNVVSAEALVRWFSPE
                     LGFLSPDEFIPLFEKSGFIIELDFYMLKKACSFVRKTFMKKNQYTYPIAVNFSRVTIY
                     QNSFYQRFLDTVKEYEIPFKYIEIEVTESAFNEISQPVISILEELKKLGFLISMDDFG
                     SGYSSLSLLCSLSINGLKLDKSLLKETFNREKVYSIIQCIIEMSHRIGMSVVCEGIET
                     KKDLEFLNTVKCDVGQGFYFSKPIEEKEFFNKYVTKK"
     gene            complement(2280..>2840)
                     /locus_tag="MJE33_003580"
     CDS             complement(2280..>2840)
                     /locus_tag="MJE33_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649486.1"
                     /translation="NQFEKSYDIGIFKNEQSIYKFIAKIPFVKKDNSNFNYTNYYMEF
                     EDIPEYYEVNFNNYLYIITRFSFIPDGTEIFFTWEEIHCWDEKVATEKTFINGETLVD
                     SYNFPNNEVKDYIQKRESLYIETKKYYEEKGLKIERHALGSEDGEYVELTNDSILYLL
                     DIEAVEIREQSKNIEEFLYKYKKRFK"
BASE COUNT         1072 a          293 c          406 g         1069 t
ORIGIN      
        1 aaagagttaa attgtaggta aatagtattt taattattta ttaaaatata gattatgtat
       61 atgccaaatt taaaaagaga cataaagaca ttatacaaat atattattgg ggtgatattt
      121 tataagaaaa gaagtgatgc agtaaattaa ttgagcaatt taaaaatgat ttaatgatag
      181 tgttgatgaa tgtttaaaaa gaaattttag aaaaacgtat attgctgttt gtgttggagg
      241 tgatgaattt gtataattta taggatataa atgattaaac tatttaagat aaatacatat
      301 cactgtaaaa tattgataat cattttttat aagagattga ttatttgcaa attatatata
      361 ttacaaatga ggtaacaata atgaagaaaa aatatgattt tttaatttat tctgctattt
      421 ggctagttat tattattctt tttatatttt ctctatatca cagtatagag catattaaaa
      481 ttataagtca cactggtact attcgcagtg aaactcaaaa ggttgttaag caagaattga
      541 ataatgaaag aaatgatgac ttgataaaac gactggataa tattctgata aagctaagaa
      601 caggtaatgg agaaaatgga tttcaaagat gtgataataa agaatttcaa caaaaactaa
      661 atcaaatgga ttctatgtgg gaatctatga agaaagaaat tataaaagtt agaaatggtg
      721 cttcaggtga taaattatat aaattgagtg aagagtattc tgtgttgtct aatcaaattg
      781 tttttatatc tgaaaaacac tctaatgcaa aattgtattc ttttgctact gcactattta
      841 tttatcttat attctcaaca ataagtcttt taatttggga atattataat aaaaaaaggt
      901 ttaaaagaat attttatact gataatttaa caaaaataaa aaatcaggtt gcatttgaaa
      961 acagagcgat tgaaattttg cacaatgcat ctaacaaaga gtatgtactg ctaaatattg
     1021 atattgacaa ttttaaatat attaatgata cacatggata tgaatatgga gataaagttc
     1081 taataatagt tgcagctgca ctttctaaaa cttttaatat caaagaaact tgtgcaagaa
     1141 ttggttcaga taattttgta atacttgcaa aatatagaga ttctctttta gaggatattc
     1201 gggaaatgct aactaatgct ataatctctg aattagatat gaatgtaact caaactatat
     1261 cttattgtat aggagcgtac ttagttgaaa ttgataattt aggttataaa tccatcaatt
     1321 ctataatgga taaggcaaat attgcacaca aggtaagtaa aacaagagga atttcctcca
     1381 cagtatggta taatgaaaat ctgttaaaac agttacaaat ggaaaatagt atttataatt
     1441 atatgtataa agcgatagaa aatgaagaat ttcatatgta tttgcaacct aaatttcaaa
     1501 tatcttcttt aaatgttgta agcgcagaag ctcttgtaag atggttttct ccagaacttg
     1561 gttttttatc accagatgaa tttattccat tgtttgaaaa aagtggattt attattgaat
     1621 tggactttta tatgttaaaa aaagcgtgtt catttgtaag aaaaacgttt atgaaaaaaa
     1681 atcaatatac atatccgatt gcagttaatt tttcaagggt tactatttac caaaatagtt
     1741 tttatcaaag gtttttagat actgttaaag aatatgaaat tccttttaag tatatagaga
     1801 tagaggtaac tgaaagtgct tttaatgaaa tatctcaacc agttatttca attttagagg
     1861 aacttaaaaa gttaggattt ttaatttcaa tggatgattt tggttcagga tactcttcat
     1921 taagtctttt atgttctcta tctattaatg gattgaagtt agataaaagc ttacttaaag
     1981 aaacctttaa ccgggagaaa gtatatagta ttattcaatg tattattgaa atgtcacatc
     2041 gtataggcat gtctgttgta tgtgaaggta ttgaaactaa aaaagacttg gaatttttaa
     2101 atacagttaa atgtgatgta ggacaagggt tttatttttc taaaccgata gaagaaaaag
     2161 aattttttaa caaatatgta actaaaaaat aatataatta tttttatcag tctatacatt
     2221 gagtcatttt atatctcgtt aaatgactca atgtactgtt atgtatttta aaatataaat
     2281 tatttaaaac gttttttata cttatataga aattcttcta tattttttga ttgttctctt
     2341 atttcaactg cttctatatc tagtaaataa agtatagaat catttgtaag ttcaacatat
     2401 tcaccatctt ctgaaccgag tgcatgtctc tcaattttta atcctttttc ttcataatat
     2461 ttttttgttt ctatatataa tgattctcgc ttttgtatgt agtctttcac ttcattattt
     2521 ggaaagttat atgaatccac taatgtttcg ccatttataa aagttttctc ggtagcaact
     2581 ttttcatccc aacaatggat ttcttcccaa gtaaaaaata tctcagttcc atctggaatg
     2641 aatgaaaacc tggtaataat atataaataa ttattgaaat ttacttcata atattcagga
     2701 atatcttcaa attccatata gtaatttgta taattaaaat tactattatc tttttttaca
     2761 aagggtattt ttgcaataaa tttatatatt gattgttcat ttttaaaaat tcctatatca
     2821 taactttttt caaattgatt
//
LOCUS       DAGSRM010000174         2793 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.180, whole genome shotgun sequence.
ACCESSION   DAGSRM010000174 DAGSRM010000000
VERSION     DAGSRM010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.180"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..254)
                     /locus_tag="MJE33_003581"
     CDS             complement(<1..254)
                     /locus_tag="MJE33_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9649487.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKIS"
     gene            complement(1063..2100)
                     /locus_tag="MJE33_003582"
     CDS             complement(1063..2100)
                     /locus_tag="MJE33_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBY9649488.1"
                     /translation="MIKSKKILSLIIAGVLGVSMLTGCSQNDGSNASNENKETDSKKQ
                     KNIGITQLVEHPSLDKAKKGFIKALEDKGYKDGDNIKIDFQNAQNDMPTTQSIASKFV
                     SDKKDLIYAISTPSAQAAYNATKDIPIIMTAVTDPVEAGLVKSLEKPGGNVSGTSDYL
                     SIDKTLELVKTLTPKAKKIGVIYNTSEVNSKIQVDSLHDYAKKNNYEVVEKGISSSSE
                     VNQAISSLVGKIDVLYVPTDNLIVSSMPIVSKVANENKIPIIASEEGSVSSGALACCG
                     IDYEKLGYKAGELAIEVLEGKSVGDIPVTTLDETEIIINEDTLKALDMQKLSADNIKY
                     IKSDENAKSAK"
     gene            complement(2197..>2793)
                     /locus_tag="MJE33_003583"
     CDS             complement(2197..>2793)
                     /locus_tag="MJE33_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003399531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBY9649489.1"
                     /translation="GLIKESSGQITLDSTTITNLAEYKRTQIVSRVFQDPSLGTCPSM
                     TVRENLSLALNKGKLLNLKKCLRHKTNDLEHLLEGISLDLKKYLDIKVQYLSGGQRQS
                     LSLIMSSLASPKVLLLDEHTAALDPKTSNEVIELTDKIVREKNITTLMVTHNLKHALQ
                     YGDRLIMLHKGEVVLDVKDKEKEDLTVEEILEKFEYAV"
BASE COUNT          937 a          431 c          364 g         1061 t
ORIGIN      
        1 ttagatatct taaacttaat gtgaagaatc cctgtaacaa gaccagcagt acaaccacat
       61 aagatagcca ttaaagttgc tactacagga gaaataccat tagtaagaga aaatgctaca
      121 atagaagcac ccattgtgta gctaccatca gcagacatat ctggaaaatc taagattttg
      181 taagttatat aaacccccag tgccataatc gataaaatta aactttgcgt cattacagat
      241 ataatacctg acataaataa aacactcctt tgaaaatatt tttataattt taattatttg
      301 ctaattttgt gtataaaaaa accacatagt gtacaaaact atgtggtaaa aacacgaata
      361 agccacatag tttaatctat gtggctctta tatagttaaa tgaaaacttt ataaacaata
      421 tagccaacat agatacaatc ccaaaatttt agtagtctta aggggtctgt atctatgtta
      481 aaacgtaggt acaaacccta aaaatagtgg ctataataat aataatattt tctattgttt
      541 aataaagtat tcataattat ctctcccttt taatatttta attagtcaga aaattatatg
      601 tattctcgac ttgttataaa ttataaatta tacaataata aaagtcaact gctaaattta
      661 attaaaatac taaataatgt atactcagaa tacttttaaa ttaatgtatt gtttgcaaaa
      721 aacgttatcc taagttataa gtgaatgtgg aaattttatc aaaatattta ctagatatgg
      781 aataactaga catgtatatc aatcaaatat cataaaaaaa agctgtctgg agaatattaa
      841 aattcttttg gacagctcaa gtagtgatta gaaagttata taactttcat aatcagtcgt
      901 caatatagac gacttttatg gaaaatgaat aactttatat gcaatattca aattgctaca
      961 ataagcacaa ttttcaaagg gttagggtgg ataagagtac ttattatata aagttttcat
     1021 attacctact tcatattacc tattttttga aattaatagg ttttattttg cagattttgc
     1081 attttcatct gactttatat actttatatt atctgctgat aacttttgca tgtcaagtgc
     1141 ttttagtgta tcttcgttaa ttattatttc agtttcatct aatgtagtaa ctggtatatc
     1201 gccaacagat tttccttcta atacttcaat agcaagttct ccagccttat aacctagttt
     1261 ttcataatct attccacaac aagctaaagc accagaagac acagaacctt cttcagaggc
     1321 tattatagga atcttgtttt cattagcaac tttagaaact attggcatag aagaaactat
     1381 taaattgtca gtagggacat ataaaacatc tattttgcca actaaactag aaatagcttg
     1441 gttaacttca cttgaagagc tgattccttt ttcaactact tcataattat ttttcttagc
     1501 ataatcatgt agagaatcaa cttggatttt tgaattaact tcactagtat tgtatataac
     1561 ccctattttc tttgcttttg gagtcaatgt tttaaccaat tctaatgttt tatcaattga
     1621 aagataatca gatgtaccag aaacatttcc acctggtttt tcaagagatt taacaagtcc
     1681 agcttctaca gggtctgtaa cagcagtcat tattatcgga atatctttag tagcattata
     1741 agctgcttgt gcagatggag tagatatagc atatatcaaa tcttttttgt cggatacaaa
     1801 cttactagca atactttgtg tagtaggcat atcattttgt gcattttgga aatctatttt
     1861 tatattatct ccatctttat agcctttatc ttcgagtgct ttgatgaatc ctttctttgc
     1921 tttatccaga gatggatgtt ccactaattg agtaatacct atattttttt gttttttgct
     1981 atctgtttct ttattttcat ttgaagcatt ggagccatca ttttgagaac atccagtaag
     2041 cattgataca ccaagtactc ctgctataat taaacttaat atttttttac tttttatcat
     2101 tttattttcc tcccttaaaa tttattaatt atatttgttc atatattcat atgatagatt
     2161 ataaatattt ctgattaaaa ctaagatttt aatttcttat actgcatatt caaatttttc
     2221 taagatttct tcaactgtta aatcttcttt ttctttatct tttacatcta atacaacttc
     2281 acccttatga agcattatca acctatctcc atattgaaga gcgtgtttca agttgtgagt
     2341 aaccataagt gtagttatat ttttctctct tacgatttta tctgttaact ctattacttc
     2401 atttgatgtt tttgggtcaa gtgctgctgt atgttcgtct agtaataaga ccttaggact
     2461 tgctaagcta gacataatta gtgatagtga ttgtctttga ccccctgaaa gatattgtac
     2521 tttaatatct aaatattttt ttaaatccaa agaaattcct tcaagtaagt gttccaagtc
     2581 attcgtttta tgacgaagac atttttttaa gttcaataat ttccctttat taagtgctag
     2641 agataaattt tctctaaccg tcatagaagg acaagttcca aggcttgggt cttgaaaaac
     2701 tctacttact atttgagttc ttttatactc agctaaattt gttatagtag tactatctaa
     2761 agtaatttga ccagaagatt cttttataag ccc
//
LOCUS       DAGSRM010000175         2720 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.181, whole genome shotgun sequence.
ACCESSION   DAGSRM010000175 DAGSRM010000000
VERSION     DAGSRM010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.181"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            217..1635
                     /locus_tag="MJE33_003584"
     CDS             217..1635
                     /locus_tag="MJE33_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBY9649490.1"
                     /translation="MTELMIISGLVLLICITSSKVLYKFGVPILLIFILLGMLFGSDG
                     MVGIYFDNYELTKQLCSIGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITAGVT
                     GMFCHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLKGSLASLLEVESGSNDP
                     IAYMLTLILLTLMNNSGVELIAPMVIKQIAFGLAIGFILAKLSVYILRHSNFEIEGFY
                     TIFITAIAILSYAFSEYLGGNGYLSVYISGIIIGNSRIPLKKSLVHFFDGISWIMQIM
                     LFFMLGLLSFPSKLPNVFGIAMAISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVG
                     LRGAASVVFAIYAVTQGVAIENDIFHIIFFIALLSVGIQGSLIPTVARKLDLVDDNTP
                     VLKTFNDYDGEISSKLIEVNVDEGNKWANKSIIDSNIPDDILIVMIKRKEQVLIPKGS
                     TIIKTGDILVLSGDRIEELIQL"
     gene            1711..2523
                     /locus_tag="MJE33_003585"
     CDS             1711..2523
                     /locus_tag="MJE33_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBY9649491.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLAEIKKAVENPEEVENILMNKEKEMLDVIKSEEYKIIKLRN
                     YLMNLKGEVNMNDVIIKSLPKVKVASMRKVLKGYDELFNLFPNVMAEEMMKTGCTCAV
                     PDYCFNIYHDGEYRETDMDVEMCQAINDLKEDTDILKFKELDAVENAVCILHNGPYST
                     LGKTYADAFKWIEDNKYKVVGNPRESYIDGIWNKESEEDWLTEVQIPVKRML"
BASE COUNT         1012 a          277 c          483 g          948 t
ORIGIN      
        1 taataaaaaa aaattcaaat tataaatatt aaatgaatat aattaaaaca aatatataaa
       61 tttaagtata acactcacat tttaatgtat tgtgaaattt ttgttgaact ttatgtacca
      121 taacttataa aatttatgtc atgttatatt tgaataagta ataaaattgt agatgtaaat
      181 taatctacaa aatttatatg aaatggagtt gatgctatga cagaattaat gattataagt
      241 gggttagtac tattgatttg tataacttct agcaaggtat tgtataaatt tggagtacca
      301 atattattga tttttatctt actagggatg ttatttgggt ctgatgggat ggtaggaatc
      361 tattttgata actatgaact gacaaaacaa ttatgttcta ttggactaat atttataatg
      421 ttttttggag ggtttggaac aaactggaat atggctaaac ctgttgctat accttctata
      481 ttaatgtctt cactgggtgt aataattacg gctggcgtta caggtatgtt ttgccacatg
      541 gtaataggaa ctagtttact tgaaggttta ttaattgggg caattgcagc ttcaacagat
      601 gctgcatctg tgtttgctgt gttgcgttct caaaaactaa acttaaaggg ttcacttgca
      661 tcattattag aagttgaaag tggaagtaat gacccaatag cgtatatgtt gacactaatt
      721 cttttgacat tgatgaataa ttctggggta gaacttattg caccaatggt aataaaacaa
      781 atagcttttg ggttagctat aggatttata ctagcaaaat tatctgttta tattttaaga
      841 cattcaaact tcgagataga aggattttat actatattta taacagctat tgcaatactt
      901 tcttatgcat ttagcgagta tcttggagga aatggatatt taagtgttta catttcaggt
      961 attattatag gaaatagtag gatacctctt aaaaaaagtt tggttcattt ctttgatgga
     1021 atttcatgga ttatgcaaat aatgttattc tttatgttag gattgttatc tttcccttct
     1081 aagttaccaa atgtatttgg tatagcaatg gccatatcta tattcatgat agttattgca
     1141 agaccattag caacatttat agttctagca aaatttaaat tctcaaataa agaaaaatta
     1201 ttcatatcgt gggtaggtct tagaggtgcg gcttcagtag tttttgcaat ttatgctgtt
     1261 actcaaggag tagccataga gaatgacata ttccatataa tattttttat agcgttgctt
     1321 tcagtaggaa tacaaggttc attgatacca actgtagcca gaaagttgga tttagttgat
     1381 gataatacac ctgtattaaa aacatttaat gattatgatg gagaaattag ctctaaactt
     1441 atcgaagtaa atgttgatga gggtaataaa tgggctaata agagtataat agattctaat
     1501 ataccagacg atatattaat agttatgata aaaagaaagg aacaggtttt aatacctaaa
     1561 ggttcaacca ttataaaaac aggggatatt ttggtattga gtggagatag aattgaagaa
     1621 ttaatacaat tataatttat tttgtattga ctttaaccta aggggaaagt ttaaacttaa
     1681 attaatcaaa tctaatgcgc aggtgatgag atgttttcta taggaatgtt ttctaaaata
     1741 aataaaataa ctacaaagac attaaggtat tatgaagata tagggttgtt aaaaccagaa
     1801 tatgtagatg aatttacaaa atataggtat tatacaacag agcaattacc taaattacat
     1861 cagataataa ctttaaaaca aatgggttta tcattagctg agataaaaaa agctgtagaa
     1921 aatcctgaag aagttgaaaa tattttaatg aataaagaaa aagaaatgtt ggatgtaatt
     1981 aaaagtgaag aatataaaat aattaaacta cgtaattacc ttatgaattt gaaaggagaa
     2041 gtcaatatga atgatgtaat tattaagtct ttaccaaaag ttaaagttgc ttctatgaga
     2101 aaagtgttaa aggggtatga tgaacttttt aatctatttc caaatgtaat ggctgaagaa
     2161 atgatgaaaa cagggtgtac atgtgcagtt cctgattatt gttttaatat atatcatgat
     2221 ggtgaatata gagagactga tatggatgtt gaaatgtgtc aagctataaa tgatttaaag
     2281 gaagatacgg atatattaaa atttaaagaa ttagatgcag ttgaaaatgc agtttgtata
     2341 ttacacaatg gtccatattc aacattagga aaaacttacg cagatgcatt taaatggata
     2401 gaggacaata aatataaagt ggttggaaat cctagagaat cttatataga tggtatatgg
     2461 aataaagaga gtgaagaaga ctggttgaca gaagttcaga taccagtgaa acgcatgtta
     2521 taatatgtct acaaagtata aataagaata aaaaaggtgg tgtctcaaag taaacatttt
     2581 agtttatgag acactctttt tcatatgaaa tcaagtatat ttttataatt acgattatga
     2641 aaaaaggctt atctatattt ttagacagcc tcttattttt ttattataat agttttctaa
     2701 agatgctcta tttataaatt
//
LOCUS       DAGSRM010000176         2524 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.182, whole genome shotgun sequence.
ACCESSION   DAGSRM010000176 DAGSRM010000000
VERSION     DAGSRM010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.182"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(73..543)
                     /locus_tag="MJE33_003586"
     CDS             complement(73..543)
                     /locus_tag="MJE33_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBY9649492.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYKELFEQEIELDLGKNVTFKGGFAI
                     QEDFAWLTGINPDTIIQKSNNMELYFEVDDFDEFIEKLNYYKDVEFVHKPLKHEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLSEGYSVEETSKIIQHPIKFVKQCENSKE"
     gene            complement(1141..1530)
                     /locus_tag="MJE33_003587"
     CDS             complement(1141..1530)
                     /locus_tag="MJE33_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBY9649493.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYQWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEKDV"
BASE COUNT          800 a          459 c          156 g         1109 t
ORIGIN      
        1 actagtccta tatattatct tgaattagtt ctattacaat taatttctac taaataatta
       61 taatttaaac aatcattcct tactattttc acactgcttt acaaatttaa ttggatgttg
      121 aattatttta gaagtttctt ctacagagta tccctcagat aaatatcttc tagctataac
      181 ttccatagac tctcctatct caataatatg cttatccggg tcataaatac gcactactct
      241 ttgttgccat tcatgtttta gtggcttatg gacaaactct acatctttat aataattgag
      301 tttttcaata aattcgtcaa aatcatcaac ctcaaaataa agttccatat tatttgattt
      361 ttgtataatt gtatctggat taatccctgt aagccatgcg aagtcttctt gtatagcaaa
      421 tcctccttta aatgttacat ttttacctaa gtccaattca atttcttgct caaataattc
      481 tttgtaaaat ttttttgata cttcaatatc cttcactgct aataatgaaa cctgatattt
      541 cataaatttt cctcctaatt ttttatttct attttttcag tatcttctat taacattttt
      601 ttctctatct cttcagtacc ttttattaat cctttatttt tactttttta gtatcttcta
      661 ttaacacttt tgtttctacc tcttcagtat cttatatcaa tcttttattt tttcaatatc
      721 ttctgttaac acttttatct ctatctcttt aatatcttat attaatcctt tatttttatt
      781 ttttcaatat catcattaat tcctatttat tttatcatct taaatacgaa aacactgtgt
      841 catatttgca aaatatatat tatcatctat attaaagctt cacaatttat atttaaaaat
      901 tctctttaat tacactacct ctatttaaaa agctacctta attatactta taagtaaagg
      961 tagcttttta aatttaatta aatagaatat ccactattaa tttatattta atttatttag
     1021 attcacatta tagtatcata atccacttat aatcatttgt catattactt aagtagacta
     1081 atatattaca taaatacact tacatacact ttatattgat gctataagaa atacgtttgc
     1141 ttatacgtct ttctcctctt cttttagatt ttttatccac tcttcaataa gtaaaatatc
     1201 atctttgctc aaattaatat tttcatgtaa tgacaacaat aaacttttta tagaagaatc
     1261 atgaatattt ctaaaaaaat ctcttgtctc aactcctatg tattcctttt ctttaattaa
     1321 aacttcataa tgcgtatatt tatctatctt ttgagaattt agaaatcttt ttactactaa
     1381 tctagataaa agagtcaaag ttgtcgtttg tttccattga tatttttgct ccattcctaa
     1441 aacaatatct ctagatgtta ctttggaatc tgatttccat ataaatttca ttatctttaa
     1501 ttctgattgt ggcaactttt ttatagtcat gataaaacct tgctactcag aatatagaca
     1561 agcaatatcc ataataaaat ttttcgatta aatacatctt atacggattt atataagctc
     1621 agacttataa gatatttttc aatcttaatc ctaaatttag ctatttgtac acggtaaata
     1681 tatataaata atgtcaaaat tctcttgtat tatattgctt aattttatga aatattttgt
     1741 actcagctat ctattttgga tacttgtcca gtagctctcc tttcttttaa actctttgat
     1801 tttaaatatt tgtatttcag taaatcctac ttcctaaatt ttttatttac tacaacttat
     1861 tactgaactt cctacttcct aaacttttta tttactacaa cttattactg aacttctcac
     1921 ttactaaatt tcttacttac tataaattat tactaaactt cctactttct aaatttctta
     1981 cttactataa attattacta aacttcctac tttctaaatt tcttacttac tataaattat
     2041 tactactaaa cttcccactt actaaatttc ttacttacta taaattatta ctaaacttcc
     2101 tactttctaa atttcttact taccacaaat tattactaaa cttcctactc cctaaatttc
     2161 ttacttgcta taaattatta ctaaacttcc tactttctaa atttcttact tgctacaaat
     2221 tattactaaa tttcttactt gctataaatt attactaata aacttcccac ttcctaagtt
     2281 tcttacttac tactacttat aactaaactt cccacttcct aaatttctta cttactacca
     2341 cttataacta aacttcccac ttcctaaatt tcttacttac taccacttat cactaaactt
     2401 cccacttcct aaatttctta cttactacca cttatcacta aacttcctac tttctaaatt
     2461 tcttgcttct taagaataat gttaatttta attttaagat tatagttttt accttgttta
     2521 attg
//
LOCUS       DAGSRM010000177         2475 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.183, whole genome shotgun sequence.
ACCESSION   DAGSRM010000177 DAGSRM010000000
VERSION     DAGSRM010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.183"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(235..990)
                     /locus_tag="MJE33_003588"
     CDS             complement(235..990)
                     /locus_tag="MJE33_003588"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBY9649494.1"
                     /translation="MLKVIISGYNGTMGRVLAECVKKDNELELVCGVARSADEKGHHS
                     DTKLYKTMTDVAEKTDVIIDFSHHETLEPILSYALKNNTPVVLATTGYDENDLNKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIREVLPESENTHGRYGRDCKRKSNEIGIHSIRGGNIISDHDVVFAGDNEVLTLSHH
                     SQSDEVFANGSIVAAKFLIGKQSGLYKMSDILG"
     gene            1430..2419
                     /locus_tag="MJE33_003589"
     CDS             1430..2419
                     /locus_tag="MJE33_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBY9649495.1"
                     /translation="MLKEEKTYQEKDAKVFNGVAALIMILLFLVVSIGIMVFGIVKLD
                     SGFTGIGSLMLILGLVFIVVDFILFFGLKMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAVSRINVSKSSSDEKVDISTNSSGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVIDATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVCEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
BASE COUNT          912 a          314 c          364 g          885 t
ORIGIN      
        1 catataccaa taaataggat aatatttaat aggtttatat gatgaagaga ttataaaata
       61 ataaacaata taattccata taccaataaa taagataatc tttaagaaaa atgattatat
      121 ttaaaaggta aacataaaat ttataaactt ttagaataga aatctcaatc aattagatat
      181 tcaaattgat gaatatgaat acctaaatag attgagattt attatgtaca aaacttatcc
      241 taatatatct gacattttat aaagcccaga ttgcttacca attaaaaatt tagctgcaac
      301 tatagaaccg tttgcaaata cttcatcaga ttgagaatga tggcttaatg taagaacttc
      361 attatcacct gcaaatacaa catcatggtc gctgattata ttgccacctc ttatagagtg
      421 tattccaatt tcatttgatt ttcttttaca atctctacca tatctaccat gtgtattttc
      481 actttcaggc aagacttctc taattgcatt agctatcata gtagcagttc cactaggggc
      541 atcttctttt ctattgtgat gtttttctat gatttcaata tcaaaacctt ctaacatctt
      601 tgaagcatcc ttaactaatt ttattaatat attcactcca agagatgtat ttgatgataa
      661 taatatagga atgttttttg atgcttcttc tattttattt aagtcatttt catcatatcc
      721 agtagtagct aatactacag gtgtattatt ttttagagca tatgacaata ttggctctaa
      781 agtctcatga tgtgaaaagt ctataattac atcagttttt tcagctacat cagtcattgt
      841 tttgtaaagc tttgtatcac tatgatgacc tttttcatca gcagacctag ctacaccaca
      901 aaccaactct aattcattat ctttttttac acattctgct aaaactcttc ccattgttcc
      961 attatagcca ctaattataa ctttcaacat aaattcctcc tatgaaatta ataaaaattt
     1021 gttatatttc tataagttta ttttatcact aatataattt atataaaata atattagtga
     1081 taataagctt acaatttatc ttacattaaa aaatttaagt ggatttttca aaatatagat
     1141 aaatttcttt ttcattgtat aaatgataaa aatatatttg acttcatacc aaaaagagtg
     1201 ccccatgaac taaaaagtta attttgaaat accatattca tatatcatat gatttatttg
     1261 gaaatcaata ttaatacatt aaaataacta aatacatgta tgcataaaga tgtataaaaa
     1321 atatataaac atatagtatt aattttaaat tcaacaattt aattgttaaa agtgttatta
     1381 ttaaattaga aatatcttaa cttaatatac tgagatggga gtgatagata tgttaaaaga
     1441 agaaaaaact tatcaagaga aagacgcaaa agtattcaat ggagtggcag ctttgataat
     1501 gattttatta tttttagtag tttcaatagg tatcatggtt tttgggatag taaaacttga
     1561 tagtggattt actggaatag gttcacttat gttaattttg ggacttgtat ttatagtagt
     1621 agattttatc ctattttttg ggctaaaaat gattaatcct aaagaagcta tagttctagt
     1681 tttgtttggt aactattatg gaactataaa aaaagaaggg tactattggg tgaatccatt
     1741 ttgttcagca ataaacccag cagtatcaag aattaatgta tcgaaatctt catctgatga
     1801 aaaggtagat atatcaacta attcaagtgg caaaaaagta tctttaaaaa caatgactct
     1861 taacaatgaa aagcagaaag tcaatgattt attgggaaat ccaattataa taggagtggt
     1921 tgtaatttgg aaggttatag atgcaacaaa ggcagtattt aatgtggata attataatac
     1981 attcttatct attcagtgtg attctactat tcgcaatgtt tcaagacttt atccatatga
     2041 tgtatctgaa gatggagatg aaaaatctct tcgtggtagt agtcaggaaa tagcagatag
     2101 attaaaagat gaattacaat caagggttga tattgcagga attgaagtgt gtgaagtgag
     2161 aattactcat ctttcttatg caccagagat tgcagcagca atgttacaac gtcaacaagc
     2221 agaggctata atagcagcac gtaaaaagat tgttgagggg gctgtaagta tggtagagat
     2281 ggcattgact aatttaagtg aaaataatgt tgtgactttg gacgaagaac gtaaagcagc
     2341 tatggtaagt aatctgttag ttgtgctttg tggcaataaa gatgcacaac cagtagtaaa
     2401 cagcagttct atttattaaa ttataaaaag tttaatagtt tattttattg atatatagaa
     2461 aatttagtag ttcat
//
LOCUS       DAGSRM010000178         2448 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.184, whole genome shotgun sequence.
ACCESSION   DAGSRM010000178 DAGSRM010000000
VERSION     DAGSRM010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.184"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            216..2228
                     /locus_tag="MJE33_003590"
     CDS             216..2228
                     /locus_tag="MJE33_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBY9649496.1"
                     /translation="MKKSVALVNDSRKDLIDFLENNLKLVFGDSININRYFINEINDN
                     DIINDDVILVMSVERLDKIINNILDKKKVIVVRRTFREDKIYNLLSLPQGTNVLIVND
                     SDETTLETISLFYKIGVTNIRPIPYMNDNNYKNIKIAITPGVPEKVPSFISDIFDLGH
                     RYIDISTFIEIINLLQIDSKEIQSNLVKYSEEIISLDTGIKDKYKELFLKIEELDTIL
                     NLSKDGILFTSKDGEINTYNSKVKDILDINEDIYGKYIEDIFVDSLKVLLSEKEILDK
                     VVVFNKKYINVNKKNIYNRDEKMGTYYSLQEITYIKKLEQNLTKKLREKGQIAKYTFK
                     DIKTNSPKMFECIDLAKKVSKSDLSILIRGESGTGKELIAQSIHNNSNRKNQPFIAVN
                     CAAVPENLLESQLFGYDKGTFTGGLKDGKQGLFELANNGTIFLDEIGDMPLELQTKLL
                     RVLQEKQIMPVGSHNVINIDVRIISATNKNLEQMIDNSQFREDLYYRLNTIPINIPPL
                     RERKEDILIIMEDLINKKLVITPEAKKLIQNYMWKGNIRELQNVTSYLNIMCEDIVLE
                     KDLPPNLRSSDNKNTSLKLKYSKNDILNILEILILNKESDVGIGRGLILKALLDKNLQ
                     ITEGKIKKIFEYLKKEELIICSSGRYGSKITQKGEDFYNKLKYKGL"
BASE COUNT         1086 a          233 c          343 g          786 t
ORIGIN      
        1 gaaatgaaaa atatacatac agtttaagtt ttaagcaaca attaggggga tttggtgaca
       61 agatttttaa ttataagaaa gaaagtgtct taatgcaact tttataaagt cattatagac
      121 actttctttt ttatttgata aatttaaatt atcaaaatta tattgcttta tacaactatt
      181 ttgttggaaa ctatttatta tataaggaga aataaatgaa aaaatcagta gctctggtaa
      241 atgatagtag aaaagattta atagactttt tagaaaataa tttgaaatta gtttttggag
      301 attctataaa tattaataga tacttcataa atgaaataaa tgataatgat attataaatg
      361 atgatgtgat attagtaatg tcagtagaaa gattagacaa aattataaat aatatactag
      421 ataagaaaaa agtgattgtg gtaagaagaa cttttaggga agataaaatt tataatctac
      481 tatctttacc acagggtaca aatgttctaa tcgtaaatga ttctgatgaa actactctag
      541 aaactataag tttattttat aaaattggag tgactaatat tagaccaatt ccatatatga
      601 atgataataa ctataaaaat ataaaaatag ctataactcc aggagttcca gaaaaagtac
      661 ctagctttat ctcagatata tttgatttag gtcacagata tatagatatt tctacattta
      721 tagaaataat aaatttatta caaattgatt caaaggaaat acaatctaat ctagttaaat
      781 actcagaaga aataataagt ttagatactg gtataaaaga taaatataag gaattatttt
      841 taaaaataga agaattagat acaatattga atttatcaaa agatggtata ttatttactt
      901 caaaagatgg tgaaataaat acatataata gtaaagtaaa agatatttta gacataaatg
      961 aggatatata tggaaaatac attgaggata tatttgtaga tagtttaaaa gttttattga
     1021 gtgagaaaga gatactagat aaagttgtcg tattcaataa gaaatatata aatgtaaata
     1081 aaaaaaacat atacaataga gatgaaaaaa tgggcacata ttatagttta caagaaataa
     1141 catacataaa aaaattagaa caaaatctca ctaaaaaatt aagagaaaaa ggacaaatag
     1201 caaaatatac atttaaggat ataaaaacaa atagtcctaa aatgtttgaa tgtatagatt
     1261 tagctaagaa agtttctaaa tctgatttaa gcatacttat cagaggtgaa agtggaactg
     1321 gtaaagaact catagctcag tctatacata acaactcaaa tagaaaaaat caacccttta
     1381 tagcagtaaa ttgtgcagcc gttcctgaaa atttattaga aagccagcta ttcggatatg
     1441 acaagggaac ttttactgga ggtctaaaag atggaaaaca aggattattt gaacttgcta
     1501 ataatggaac aatattctta gatgaaatag gcgatatgcc tttagaatta caaacaaaac
     1561 tacttagagt tcttcaagag aaacagataa tgcctgttgg ttctcacaat gtgattaata
     1621 tagatgttag aatcatatct gctacaaata agaatttaga acaaatgatt gataatagcc
     1681 aatttagaga ggatttatat tatcgattaa atacaattcc aataaatata cctcctctaa
     1741 gagaaagaaa agaagacata ttaattatta tggaagattt aattaataag aaattagtaa
     1801 tcacaccaga agcaaaaaaa cttatacaaa actatatgtg gaaaggtaat ataagagaac
     1861 tccaaaatgt tacatcctat ttaaacatta tgtgtgaaga tattgtatta gagaaagatt
     1921 tacctccaaa cttaaggtct tcagataaca aaaatacatc attaaaacta aaatatagta
     1981 agaatgatat attgaatata ttagagatac taattttaaa taaggaatca gatgttggta
     2041 ttggtagagg attaatttta aaagcactac tcgataagaa ccttcaaatt acagaaggaa
     2101 aaattaaaaa aatatttgaa tatctaaaaa aagaagaact tataatttgt agttcaggaa
     2161 gatatggaag taaaataact caaaaaggag aagattttta caataaatta aaatataaag
     2221 gtctataaaa ttataattta tcaaacaaag aaatctattc aggattaaag tactccttca
     2281 ataggttttt ttatttttca agtaccctaa aattataatt tattttgtac ataaataata
     2341 ggaattaata ggaataggat aattttttta tttcctattt tttttatata aactagcata
     2401 taaaatatat tttcttcaaa ataatatttt ttactgaaaa ttgaaact
//
LOCUS       DAGSRM010000179         2445 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.185, whole genome shotgun sequence.
ACCESSION   DAGSRM010000179 DAGSRM010000000
VERSION     DAGSRM010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.185"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            271..2055
                     /locus_tag="MJE33_003591"
                     /pseudo
     CDS             271..2055
                     /locus_tag="MJE33_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
BASE COUNT         1054 a          311 c          454 g          626 t
ORIGIN      
        1 atctaatttt aaagaggttt ctaactataa tgatttcata attaatttgt aattattttg
       61 ttacattgca gtcattatgt aaggaataaa gaaattttct tattgattga aaaatttgta
      121 aatttatatt ttaaggtaag tttgattttt atgttaatga attgttataa aaaatagggt
      181 tttgtataaa aaaagtagcc tttagaatta gaacgggaac aaagttaaaa aattaagtaa
      241 tatcaaatgg acaaggggga agaaaataaa atgaaaatac caaagaagat tgctgcaatg
      301 cttacagtta caatgatagc tggaagttca acagcaggca tagctagtgc acaaactgtg
      361 gcaacaaatt taacagggca agaaagatat gaaactgctg ttaaaataag tcaagatgga
      421 tggaaaaatg ctgatgaggt agtaatcgta aacgactcat ctatagcaga tgctctatct
      481 gcaacaccat ttgcaaaagc aaaaaatgct ccaatacttt taacaagtaa agataaatta
      541 aatgataaga cgaaagcaga aatacaaaga ttaaaagcta aaaaagtata cttaataggt
      601 ggaacttctg tattaagtac aaatatagaa aaagaaataa aaaatttaaa aatatctttt
      661 gagagaatct ctggagctga gagatatcaa acatccttag aattagcaaa gaaattagat
      721 gctataagtg atgttaagaa aatagctgta gttaatggtg aaaaaggact tgcagatgct
      781 gtaagtgtag gagcaccagc tgcacaaaac aatatgccag taatacttgc tgattctaaa
      841 aatggaacag cagttgcaga caaatttata aaagatgctg gaataactca atcttatgta
      901 gtaggtggag agagttcaat ttctgaagct gttaaaaata aactaccaaa tagtacaaga
      961 ctaggtggaa cagatagaaa tgatacaaat gcaaaagtaa taaaagaatt ttataaaaag
     1021 acagatttaa aaaatgctta tgtaactaaa gatggtatga ataaacaaga ccaacttata
     1081 gatgcactag cagtaggtgt attaggtgct aaaaatcaat caccagtagt tttagttgga
     1141 aagaacttat cagcttcaca aaaatctcta gttaactcta agagttttga taagataact
     1201 aaagttggtg ggaatggtaa tgagacagca tttaatgaaa tgaagtcatt acaagaagta
     1261 aaaacagttg aagctaaaac aatttctgaa ttaaaatcag ctatagataa agctactgct
     1321 aatgatgtta taaactttaa gccaactagt gaggttaaag aagcatttac aatacaaact
     1381 gataaagcag taacagtaaa cttaaatggt acatatacaa agactgtaac tatcaacatg
     1441 cctaatggag atgtaaataa ctatgcaaaa gtggatgatt tagttataga tgatgtaaaa
     1501 gatggaactt ttgttaacta tggaaaaata actaacttaa aggttaatga taaaaatgga
     1561 gccaagatag aaaataattc taaaggtgag attggtagtt taacagtagc atctggagca
     1621 agtcaagtaa aagtgactaa tggtggtaag ataactacag ttacaaataa ttctaaaggg
     1681 actactatag ataataaagg tacaataagt agtgttaagg gagataattc tccaacaata
     1741 agtggaaata gtccaagttc taattcatca ggaggaagtt catcaagtgg aggttcttca
     1801 catggaggag gctcatcttc aggaggaagt tcttctaatc aaacatcagt aaataatgaa
     1861 gcagcaaaaa taacaagcgt gcctacacca gcaaaagatg caactagatt aactatgcca
     1921 tcagtatcaa gtggatatac tatagcaata aaaacatcaa gtaatgagag tgttataaaa
     1981 aaagatggaa caataatacc tccaaataca gcaacaacag taaaattagt atttacggta
     2041 acacatacat caagtggtaa aacagcagat acaaaagaac tatctgtaac agtaccagct
     2101 aaatcaacag atgaagaatt acaagcagca ttagacaatg aagtggcaaa aataacaagc
     2161 gttcctgcac cagcaaaaga tgcaactaaa ttaactatgc catcagtatc aagtggatat
     2221 actatagcaa taaaaacatc aagcaataag agtgttataa aggaagatgg aacaataata
     2281 cctccaaata cagaggaaac agtaacattg gtatttacag taacacaaga atcaagtggt
     2341 aaaacagcag atacaggaga aatagatgta gtagtaccag ccaaatcaac agatggggaa
     2401 atacaagcag aagtacaagc tgaggcggat aaaataacaa gtgta
//
LOCUS       DAGSRM010000180         2439 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.186, whole genome shotgun sequence.
ACCESSION   DAGSRM010000180 DAGSRM010000000
VERSION     DAGSRM010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.186"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            68..985
                     /locus_tag="MJE33_003592"
     CDS             68..985
                     /locus_tag="MJE33_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649497.1"
                     /translation="MENNKCREDFRFTQEYEEDYPNTNERYYENYQVADRYYNYPNKY
                     KEPKIKQCCCKKSMREALELLRYDALRPFVNFNQFAFISDFFIVGANLVGIDLSAPPK
                     DNLSGLDGTFERFSACNCDLIDIAGRVSYPIPVPLTLEGLINTIGTIPGVAELIALID
                     AVIPPTIDLGAILDAILAAIIDFILAASTPLANVDLASLCNLKAVAFDITPADYEDFI
                     ASLGYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVIVLGNSNDSRIYFVCVDSIDYIA"
     gene            complement(1127..2308)
                     /locus_tag="MJE33_003593"
     CDS             complement(1127..2308)
                     /locus_tag="MJE33_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBY9649498.1"
                     /translation="MNKKYIKAGNLIDGTGNSIMQNKGIIIDGDTIVEIEDIRDDLNG
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKIYYDNVSDVELVVNTIKQLDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEIVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DIIVAATKTASELLRIDKNYGTLEKGKKADVIVVNGNPVENIDVLADVLAVFKLGKKV
                     R"
BASE COUNT          797 a          406 c          310 g          926 t
ORIGIN      
        1 aatattacta tatagataag cttaattatc tatacattta aattaaaata taaaaggagt
       61 gtatttagtg gaaaataata aatgtagaga ggactttaga tttacacaag aatatgagga
      121 agattatcca aatacaaatg aaagatacta tgaaaattat caagtagctg atagatacta
      181 taattatcca aataaatata aagaacctaa aataaaacaa tgttgttgta aaaaaagtat
      241 gagagaagca ttagaacttc taagatatga tgctctaaga ccttttgtaa actttaatca
      301 atttgctttt atctcagatt tctttatagt aggtgctaat ttggtaggta tagatctttc
      361 agctcctcca aaagataatt tatctggact tgatggtact tttgaaagat tttctgcttg
      421 taactgtgat ttaatagata tagctggtag agtatcttat cctattccag tccctttaac
      481 tcttgaggga ttaattaata ctataggaac tataccagga gtagctgaat taattgcact
      541 tattgatgca gttattcctc ctacgataga ccttggggct atattagatg caatacttgc
      601 tgctataatt gattttatac ttgctgcatc tactccatta gcaaacgtag atttagcatc
      661 attgtgtaat cttaaagctg ttgcatttga tattacacct gcagattatg aagatttcat
      721 agcatcttta ggttactatc ttgataaaaa acattacaaa gaatgtaatt gcaactgcga
      781 ttgtgatgat tgctgttgta ataaaggtat cctagataat ctttatatgt caaatataaa
      841 taatcaagtt actgtagtag ctggtagttt ggttctaact ggtgttgaag ttctaggtaa
      901 gaaaaatgat gttatagtac ttggaaattc taatgattca agaatatact ttgtatgtgt
      961 agattctata gattatattg cataagaatg attttaaaga gttatatcta aatataaaat
     1021 agaatctatt ttctaaagta taaaagattt aattattact aataaaaaag agtgtctata
     1081 attatttaaa aatttcttta gacactcttt tttattttta tactatttat ctaacttttt
     1141 taccaagttt aaatactgct aacacatctg caagtacgtc tatattctct actggatttc
     1201 catttacaac tataacatct gctttctttc ctttttctag agttccataa tttttatcaa
     1261 ttcttaaaag ttcagatgct gtttttgtag cagcaactat tatatccata ttatccatac
     1321 cataatctga cattagtttc atttcataat atgtttttcc atgttcatta taagaagttc
     1381 ctgcatcaga accaactgca attttaattc cttttttata tgcttttagg aatgactctt
     1441 tatgtggaat ggcaacaatc tcacactttc taagagtagc ttcagttatt ccactttctt
     1501 ttcctgcatt tagaataaaa tatggtgcag ccacagttgg aactaagtaa gttcctcttt
     1561 caagcatcaa atcaattatt tcatcatcta aataaacacc atgttctata gaagtaattc
     1621 ctgcaagcac tgcatttttt ataccagttc ttccttgtgc atgtgtggca gttattttac
     1681 ctgcttttat agcttcatct acagcagcct tcatttcctc caaagttaat tgtggtgacc
     1741 ctggctcaac tccatcagtt gttacacctc cagttgccat tatctttaca caatctactc
     1801 cattttttaa aactgttctg gctgattttc tacattcatc tactccatca ctttcaatac
     1861 caaaataata tccatgccct cctgtcatac atataactgg acctgaagta actataccag
     1921 gaccatctat agaccctttg tctataagat ttttaagttg tacatccaca tatttgggac
     1981 aacctaagct tctaatatat gttactcctg aatccaaata agcatcaagt tgttttatcg
     2041 tatttactac aagctctaca tctgatacat tatcataata tatctttgga tttccaactg
     2101 gctccattgt caagtgaaca tgacaattca taatgcctgg cataacagtt ttatctaagt
     2161 aatcgtacac atcatatcca tttaaatcat ctcttatatc ttcaatttca actatagtat
     2221 ctccatcaat aattataccc ttattttgca taatactatt tccggttcca tcaattaaat
     2281 tccctgcttt aatatatttt ttgttcatct taaatttctc ccccttttaa gtacaatgta
     2341 ttatttattt attatttcta atattctaat aattaatttt accactataa gcattctatt
     2401 tcaaatttaa gtgcattaat ataaattaaa ttgttataa
//
LOCUS       DAGSRM010000181         2430 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.187, whole genome shotgun sequence.
ACCESSION   DAGSRM010000181 DAGSRM010000000
VERSION     DAGSRM010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.187"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            322..1623
                     /locus_tag="MJE33_003594"
     CDS             322..1623
                     /locus_tag="MJE33_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBY9649499.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDRKSLAIKTSDVTNLITWGQNFGLDGHIKILKENLA
                     RRSINQNCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKID
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
     gene            1699..1974
                     /locus_tag="MJE33_003595"
     CDS             1699..1974
                     /locus_tag="MJE33_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBY9649500.1"
                     /translation="MLELRKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELMIKLFNGKNYMELARMFEMSVINVRHIVRKK"
BASE COUNT         1002 a          196 c          473 g          759 t
ORIGIN      
        1 gagtaatgaa ttatattgaa cgatatatca ataattggca gtaagttata tcaaataaga
       61 tattactatc gagtaatgaa ttatattgaa caatatatta ataattggca gtaagttata
      121 tcaaataaga tattgctatt atgtagtaaa ttatataaaa caatgtatca gaataaatag
      181 tatattaata atttgtagta tatcatgacg aataatacag tcataacaaa tagtgagtca
      241 taacaaataa tgtatttata attgataatg tatttaaaaa tcaaaatata aaaacttata
      301 aggaaagaga gattaattta tatgggtaaa tcaaaagatg ttgaaataga gaagtctatt
      361 ttaggcacaa ttcttttaga caataaatta agctataagc tagatgaatt aaatgaaaac
      421 atgtttatga atgatatatg tcttgagata tttaaaatta tgaaagaact taaaaaagaa
      481 aacatagtga ttgatgtggc cactgtgaaa tcaaagatag atagaaaatc tctagcaata
      541 aaaacgagtg atgtgactaa tctaattact tggggtcaga attttggtct tgatggtcat
      601 attaaaattt taaaagaaaa tttagctaga agaagcataa atcaaaactg ccaaaacctt
      661 ttacacagcc taaaccttgg agaaaatatc gatacttgta tttacaaatt tgaaagtaat
      721 ataaaggaga ttttggacaa ggatacttat gaaaatgatg atgtaaatag tatagcaggg
      781 aaggtgcttg attttttaga aaacaaaaaa gatattggat ttaaatttgg tataaaatta
      841 cttgatacca ctataggagg tctttttaaa ggagagctaa ccacaatagc agctaaatcg
      901 ggggttggga agactgcttt ggcacttcaa ataatgttaa attcatttaa gcaaggtaaa
      961 aaaactttat ttataagtag agaaatgacc agtgagcaag tatttatgag aaatatatgc
     1021 agagtaactg gtgtaagtac aaggaatatg aaatctaaag aaatagatga aaatgattgg
     1081 aaacttattg taaatgctat aggtgattta agcgaaaata acttaattta tatcaatgat
     1141 aagatagata caattagtgc aattagaaag aggataaggc aggtaaagcc agatttatta
     1201 atagtggatt atgtgcaact ccttacatct cagaaaagca tggataagag agaaagggaa
     1261 gtagctactt tttcgagaga acttaaaaat atgactttag attttaatat acctgtaatc
     1321 caattaagtc agttaaatga tgaaatgaaa gattcaagac cttatggaga tagacctatg
     1381 agggatagca aagccatcta ccacgatagc aacaatgtgg tttatataca tcaattaaag
     1441 gggagtgatt atgaagaagc agtgagagat attggagaaa gtgaggaggc agttagagca
     1501 tcagaataca ggggaattaa gatggttgat ttaatagttg cgaagtgtag agatggacag
     1561 actaggcata agcatttttg ttactttggg gataagttac attttcaaga actaaattat
     1621 taggtcaata aagtagtaat aacaagctta ttaaaagtcg ggaaatagca aatagattat
     1681 aaaatatgag gtgaaaatat gttggaatta agaaaaatta tagaattact tgaagaacac
     1741 aagatagaag taagtattcc aaaggagtta cataagtata gtttattgga ataccttgaa
     1801 ttgtgtcaac aaagtggggg aacatatttt tatattaaaa cactggaagg ggctttaaaa
     1861 gaggctagaa atgagcttat gattaaattg tttaatggga aaaattatat ggaattagcg
     1921 aggatgtttg aaatgagtgt tattaatgtc aggcatattg ttagaaaaaa atagatattt
     1981 ttggagaata caagtgtgat atacttctga aatagaagtt attatagttg aaataaaatt
     2041 gtggttaata aattaaagtt gtgtataagt ttaagattat agaggagtat aagtgtgatt
     2101 aacaacatga ttttgtgaat aaaattgagg atatatatga gttaaatgtg tgattaataa
     2161 ggtaaggttg tgtataagat tatgagtatt taaaaggagg ggctataatt aatagtgtag
     2221 agttgtgtgt ttgcttaaga ttatataata atttaataga ttaaactata ctaataaggt
     2281 aaaatcgtgt gtttagttaa agcttattat aaaatcttga tatgtgatta atgatgcaat
     2341 atgtgaataa agttaagatt ataatagatt taacctgtgg ctaatagaat aaaattgtga
     2401 ataaagttaa gattataata ggtttaacct
//
LOCUS       DAGSRM010000182         2233 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.188, whole genome shotgun sequence.
ACCESSION   DAGSRM010000182 DAGSRM010000000
VERSION     DAGSRM010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.188"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            92..232
                     /locus_tag="MJE33_003596"
     CDS             92..232
                     /locus_tag="MJE33_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649501.1"
                     /translation="MEKSNTTVTKKEKIEDIGMKLEHLLSEDITFYELLLEILYKEKE
                     EN"
     gene            complement(229..600)
                     /locus_tag="MJE33_003597"
     CDS             complement(229..600)
                     /locus_tag="MJE33_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY9649502.1"
                     /translation="MESLGDRIANLRKELDINQKELATKVGITEASLSRYENNLREPK
                     SEIIVRLAKALETSTDYLLGVNDNTKISKEDKLIIENLSVSEKTKKLLEKIYSLEKED
                     REAIEKMIDNAYLKRFLKEEN"
     gene            1149..1406
                     /locus_tag="MJE33_003598"
     CDS             1149..1406
                     /locus_tag="MJE33_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649503.1"
                     /translation="MIIIRSQDKTNLMQINQIKIDGSQVYAVFESKIDTVKIGDYENN
                     TRAIQVLDNIQNFIENGTKYDYITSNKVRYNVNKIFQMPAK"
     gene            complement(1516..2196)
                     /locus_tag="MJE33_003599"
     CDS             complement(1516..2196)
                     /locus_tag="MJE33_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBY9649504.1"
                     /translation="MIWTFYSFICVILPCLIYQIVVIKRKNLKVEKNVMIHLVWVYIF
                     LLYIYLALSKAGIGSIWDIGRYSGLFRIDEINLIPFDSVGILTYILNIIMFMPLGFLL
                     PLIWKNFRNIINVSLTGLGFSLAIELCQLFNLRATDIDDLMMDTLGAVLGYLIWVGVN
                     NLFNLIKKKDIFPSIYKNIIQDEIAITIDDDISEKITSLYKNEAIIYLILAVLGEFLL
                     FNWSIIPY"
BASE COUNT          895 a          260 c          258 g          820 t
ORIGIN      
        1 aatcaaaata caattacata aactttatta aaaatttgtt gggaatttct gactaacatt
       61 aagaacgtaa gtttgttggg ggatggtata tgtggaaaag tcaaacacta ctgttacaaa
      121 aaaagaaaaa attgaagata taggaatgaa attagaacat ttattgagtg aagatattac
      181 tttttatgaa ttattacttg agattttgta taaagaaaaa gaagagaatt aattctcttc
      241 tttaaggaat ctttttaaat aggcattatc aatcattttt tctatagctt ctctatcttc
      301 tttttcaaga gaatatattt tttctaagag cttttttgtt ttttcactta ctgataagtt
      361 ctctatgatt aatttatctt ctttgcttat ttttgtatta tcatttacgc ctaataaata
      421 atctgttgat gtttctaatg cttttgctaa tctaactatt atttcacttt taggttctct
      481 taaattattt tcataacgtg acaagctagc ctcagttata ccgactttag tagcaagttc
      541 tttttgattt atatctaatt cttttcgtag atttgctatt ctatctccta aactttccat
      601 atgtacatca ctccttaccg aaattataag ttattttgat aaaaagtaaa ataaaattac
      661 caaaaatata aaaaaaaaag gttaaaataa taaaatatat tgaaaattac catttgtata
      721 tgtataatat gaattataca aatggtaatt tgatgagagg tggtatgttg aaataagaaa
      781 ctgaattaat taataaatac atagaaagtt ttgaaaacct ttaattgaga tataaacaat
      841 atgtacaaaa ttttttgcaa gtaaaattac caaatgtata aaataaatca gtagcataga
      901 taattttatg ctttaataca ttagattatt taatttagtt ataatggtca aaaatgcaat
      961 ctacaaataa aacaaagtat tatccaaaaa gtattcttta agaaaatttt ttattagaga
     1021 tgtaattgat ataaataatc gtattaaaaa ataaaatact aagtttaaaa atttaaataa
     1081 tctataaatc aattataaaa tattatttct tagaaattaa aatgaattaa gtaaatggag
     1141 gaattgatat gataataatc agaagtcaag ataaaactaa tttaatgcag ataaaccaaa
     1201 ttaaaataga tggtagtcaa gtttatgcag tatttgaaag taaaattgat actgtaaaaa
     1261 taggagatta tgaaaataat acaagagcta tacaagtgtt ggacaatata caaaacttca
     1321 ttgaaaatgg aacaaaatat gattatataa catcaaataa agtcagatac aatgttaata
     1381 aaatatttca gatgccagct aaatagtgag tgtgcatttt aatttaataa ctaaagccca
     1441 ctttcttttg tagtatgaaa gagggcttta catatttgta aatatcgaag tacacttgtt
     1501 ttatcaataa attatttaat aaggtataat ggaccaatta aacaataaaa attctcctaa
     1561 aacagcaagt attaaatata taatagcttc atttttgtaa agagaggtaa ttttttcgct
     1621 tatgtcatca tcaattgtaa tagcaatttc atcttgaata atatttttat atatagatgg
     1681 gaaaatatct tttttcttta taagattaaa taaattattt acacctaccc atatcaaata
     1741 tcctaaaaca gcacctaatg tgtccatcat taaatcatca atatctgtag ctcttaagtt
     1801 aaacaattga caaagttcaa ttgctaatga aaaacctaat ccagttaatg aaacattgat
     1861 aatatttcta aaatttttcc aaattaaagg tagtaaaaat cctaaaggca taaacataat
     1921 tatatttaaa atatatgtta agatgccaac tgagtcaaaa ggtattagat ttatttcatc
     1981 aattctaaaa agcccactat accttcctat atcccatatg ctaccaattc cagctttact
     2041 aagtgctaga tagatatata ataaaaaaat atatacccat actaaatgta tcattacatt
     2101 cttttcaact ttcaaatttt ttcgttttat aacgactatt tggtatatta agcatggtaa
     2161 tataacgcaa ataaatgaat aaaatgtcca tatcatttta ttttgtcccc tttcaaattt
     2221 atgtttttag ttt
//
LOCUS       DAGSRM010000183         2163 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.189, whole genome shotgun sequence.
ACCESSION   DAGSRM010000183 DAGSRM010000000
VERSION     DAGSRM010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.189"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            132..1037
                     /locus_tag="MJE33_003600"
     CDS             132..1037
                     /locus_tag="MJE33_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBY9649505.1"
                     /translation="MKNSNFNILAFDGGGLKGALSISILERIVKEYPNLLNNINMFGG
                     TSTGSLIALGLAYGVSPKEIKELYSIENSKYIFNKSYAEILRPKYENKNLKEVLLSIF
                     PEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMASSAAPVF
                     FPTYNRHVDGGIIATDPSLACIIHAIDSGFKLKNTRLFSIGTGYVYNSIKADTTEWGA
                     IDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKDVAMDDCDALEY
                     LMSLGENCDIKDAFGWIKKKSFYRN"
     gene            1161..2114
                     /gene="msrA"
                     /locus_tag="MJE33_003601"
     CDS             1161..2114
                     /gene="msrA"
                     /locus_tag="MJE33_003601"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBY9649506.1"
                     /translation="MIKLATFAGGCFWCMVKPFDKYDGVIKVVSGYTGGHVENPTYQE
                     VCSGTTGHLEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFYHD
                     EEQKNIALESREHLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYYKG
                     SGRYNFVKSHWDGNNINREELKKTLTPIQFEVTQNDMTEVPFENEYYDNNKEGIYVDI
                     VSGEVLFSSKDKFDSGCGWPSFSKPVIDKSIIERSDFSQGMYRVEVRSAKGNSHLGHV
                     FDDGPKELGGLRYCINSASLKFIPRENMEALGYGEYLKLFE"
BASE COUNT          843 a          219 c          407 g          694 t
ORIGIN      
        1 ggtttgaatg taacttaaat attaaaagag gaacattgta atatgtaact tgcataaaat
       61 atatatacta tagtgaaatt attttgatta aaatttaaaa tataaggtgt tttatgtggg
      121 ggtatatgta tatgaaaaat tctaatttta atatattggc atttgatggt ggtggattaa
      181 aaggggcatt gagtatatcc atacttgaga ggatagtaaa agagtatcca aatctattaa
      241 acaatataaa tatgtttgga ggtacatcta cagggagtct aattgcacta gggcttgcgt
      301 atggtgtaag ccctaaagag ataaaagaac tttactcaat tgaaaattca aagtatattt
      361 ttaataaaag ttatgcagaa atactcagac caaagtatga aaacaaaaac ttaaaagaag
      421 ttttattaag tatatttcca gaggaactag aattaaaaga tttaaataaa ttagtaatga
      481 taccaagttt ttatatagga aatgaggaaa atgcatggaa accagtgttt tataacaata
      541 tgccaaattc ttttacaaag actagcaaag ttgtagatgt agcaatggca agtagtgctg
      601 ctccggtatt ttttccaaca tataatcgtc atgttgatgg aggaataata gcaactgacc
      661 cgagtttagc gtgtataatt catgccattg acagtggatt taaacttaag aatacaagac
      721 ttttttcaat agggactgga tatgtatata acagtataaa ggcagatact actgaatggg
      781 gagctattga ctggataatt aataaagaac ccgatttacc aattatatct attaccttag
      841 aaggaaattc tcaaatgagt cagttatttt ctcaaaaact tcttggagat aattactata
      901 gattaaatcc taagatggaa aaagatgtag ctatggatga ttgtgatgca ttagaatatc
      961 taatgtcttt aggagaaaat tgtgatataa aagatgcttt tggatggata aagaaaaaga
     1021 gcttttatag aaattagatt caatttgcaa ttgtattttt acacatgatt tcattgagta
     1081 ggtaaaaata ataaaatgca gattattaag ttacttctta gggaataaat aaagtataat
     1141 ttatttaagg agtgatgaaa atgataaaac ttgctacttt tgcaggtgga tgtttttggt
     1201 gtatggtaaa accttttgat aagtatgatg gagttattaa agtagtttct ggatatacag
     1261 gtggtcatgt tgaaaaccct acatatcaag aggtatgctc aggaactact ggtcatttag
     1321 aagcaattca aataacatat gatgatgata taataagtta tgatgaactt ctaaatatct
     1381 tctggaaaca gattaatcct acagatgatg gtggacaatt tggtgatagg ggaactcaat
     1441 ataagactgc tatattttat catgatgaag aacaaaaaaa tatagcttta gagtcaagag
     1501 aacatctgga aaatagtaaa atatttgata gtaaaatagt gactgatata attccagcaa
     1561 ctaaattcta cgaagcagaa gaaaatcatc aggattatta taagaaaaga ccattaaact
     1621 acaatatgta ttataaaggg tccggaagat ataattttgt aaaaagtcat tgggatggaa
     1681 acaatataaa tagggaagag ttaaaaaaga ctttaactcc tatacaattt gaagttactc
     1741 aaaatgatat gacagaagta ccttttgaaa atgaatatta tgacaataac aaagagggaa
     1801 tttatgttga tattgtaagt ggagaagtgt tattttcttc aaaagataaa tttgattctg
     1861 gatgtgggtg gccaagtttt tcaaagccag ttatagataa atctatcatt gagagaagtg
     1921 atttctcaca aggtatgtac agagttgaag taagaagtgc taaaggaaat tctcatttag
     1981 gtcatgtatt tgatgatggt ccaaaagaac ttggaggttt aagatattgt ataaattctg
     2041 cttctcttaa atttatacca agagaaaata tggaagcttt aggatatgga gaatatttaa
     2101 aactttttga gtaattagga atatttatta taaacttatc taaaaggtga aaatctaaaa
     2161 aaa
//
LOCUS       DAGSRM010000184         2112 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.190, whole genome shotgun sequence.
ACCESSION   DAGSRM010000184 DAGSRM010000000
VERSION     DAGSRM010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.190"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            294..632
                     /locus_tag="MJE33_003602"
     CDS             294..632
                     /locus_tag="MJE33_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY9649507.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
     gene            654..971
                     /locus_tag="MJE33_003603"
     CDS             654..971
                     /locus_tag="MJE33_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY9649508.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            1121..1798
                     /locus_tag="MJE33_003604"
     CDS             1121..1798
                     /locus_tag="MJE33_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649509.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
BASE COUNT          919 a          213 c          284 g          696 t
ORIGIN      
        1 tttcaaaata tattctacat atctaatatg taattacaat aaaaaatgct ataattaaca
       61 tattattaat tcgttttatt attgtagtat ggctatgaaa ttatgaaaat atttctctaa
      121 ttaaaaatat gatttatcca tatttgttta ttgtagatac agactataca aaaataaata
      181 gcacttattt cagtataaaa acatatatag tctatattta gactaaaaaa gtataaatag
      241 tctatataaa acaacttaaa ctatgaatat aatctaaagt ggagggataa taattggcaa
      301 atataggaaa aataatagga gatagaataa aagtctatag aacaaaattg ggatatagtc
      361 aagagtttct agcagaaaag gcaggtttac atccaacata tattggtcag attgaaagag
      421 ctgagacaaa tacatctatc aatattataa tgaaaattgc tactgcttta gacataccac
      481 ttgaattgtt atttgcaaat attattacaa cagaaggcgt tgataactct gtacctttag
      541 aatgttatga aatgattaat aaattaactg aaaaagaaca aattgcaatg ctagagttaa
      601 taaagtgcat cattaaatac aaagaaatat aagtgaggga aatagtaaca ataatgaatt
      661 atataggtaa aagacttaaa gaagaacgga aaaaagcaaa tttaacttca aaagaatttg
      721 caaatatggt tggagtatcg ccttggtata taactcaaat cgaaagtggt aaaaagaatc
      781 caagtcttaa aacattcata aaatttgtaa atatattaaa tatttctgca gatgttctta
      841 taaaagatat tacttccact ggaaaaacat atttagaaaa tgatataaat gaagagttaa
      901 aagacttaaa ttcaagagaa ctaaatctga tatcacagat tatagacatc ataaaaaaga
      961 ataaaatata attctttata ttttatataa aaaaaaaggt tatttgaaat aaataacctt
     1021 tttttgtctt tttgtgttaa cattgtctta tagtgctaat gagtactatt agaccatgtt
     1081 ctatggcctt attttaatat aaaacgattg gagataggct atgattgtga ttgaaggtag
     1141 cgataaattt aagattgcaa aagaatatat tgatgtagaa tatactcttt ttagcaaagt
     1201 aacttttagg tatgaaaagt tgaaatttaa agataatgct gaattggaaa aaattaagat
     1261 gtttaaatat aaaaatggct acatccctaa taaattaaac ctttcttttg gatatggatt
     1321 ctcttcttat aaaaagcaaa taattagaga aactgtagat actttaagat tgacagaaat
     1381 tttttcaagc gagaacatag aagatattaa atttataaaa gatggtacaa aaaaattaga
     1441 aattagcata gagaaagttg tgaaatttaa acgtcgaaaa aaaagaaatt atgtttgttg
     1501 ctattgccct gatatgtata gggacataaa actcgacaaa gaatctatca ataagatata
     1561 caacagaaaa ataaaaatag aaagagaagt taatattttt gaagatgagg atgttataat
     1621 aaacaaaaaa gtattgaagt ttccaaagtc ttggacaaag aatatgcaaa aatattggtt
     1681 aagtgaaaat aagtatccca tacattctac tgtaattgat gatgatagat ataaatgttg
     1741 taatgtacaa tatacaaaaa atagagtgat aatattatat tacatatata accattaata
     1801 tacataatta caaaaaaata atgacaaata tacataatac aaatatatct tctactttag
     1861 ataaaacagt tattatgaaa acttactatc tataaataat agaaatgcta gtataccatc
     1921 tataatctaa tgtctagtag gttttaatgg catattttaa atatgccata gttgatttat
     1981 aagattttct atatcacttt caaaatacac actaaatagt tgcttttcag atataattgc
     2041 ccaatttaaa ttttactatc tgaaaagttt tttatattta atttatatct tcaaaaataa
     2101 aataccttct aa
//
LOCUS       DAGSRM010000185         2101 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.191, whole genome shotgun sequence.
ACCESSION   DAGSRM010000185 DAGSRM010000000
VERSION     DAGSRM010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.191"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            756..1223
                     /locus_tag="MJE33_003605"
     CDS             756..1223
                     /locus_tag="MJE33_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBY9649510.1"
                     /translation="MITKFFSNILSVFLYFLLSLLGVAGIIFLCMIPMFLYDFSSDKV
                     IALIARILYFSDYFIVILFLIFIINSTKYSPFVLENVKRFKVMGCCLLINTIIECING
                     YNLNTNNIRIFGTDSGGISPLMVISFIFALMCFVIAETFDKAIKIKEDNDLTI"
     gene            1234..1449
                     /locus_tag="MJE33_003606"
     CDS             1234..1449
                     /locus_tag="MJE33_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY9649511.1"
                     /translation="MAIVVNLDVMMAKRKISLKDLAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVEDEND"
     gene            1890..2063
                     /locus_tag="MJE33_003607"
     CDS             1890..2063
                     /locus_tag="MJE33_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HBY9649512.1"
                     /translation="MKRKLSTYFKGYILGLCIAFLLFTFILKKLIIENIIGIIIGTGI
                     AFLLIYLFNKKRK"
BASE COUNT          811 a          230 c          221 g          839 t
ORIGIN      
        1 ttaatacact attaatatat tgtgatatta atattacagt attaaataaa atctaataaa
       61 attaactata aaatatctat tatataaata tccactctta tgtttgaaat ccgtcatata
      121 gttttatcct tcttatcttt tatatcaata acttttttta ataaaattga caaaactaaa
      181 gtaaaaaacc ttattgataa tgaaacaaat gatactataa gttctgatat tatagtaatt
      241 gcatataaat agacatcata aaaataatat aatattgtca ctataaaatt aaatgtaaaa
      301 atctcaaatc taactaaata cacatatagg tattttttaa gtttttaggt atttcttctg
      361 aaaatgattt attgattgat accaatttac ataatctttt taatataaac aatgatacaa
      421 tatatgggac agcacatatg tatatagcta cttatattac atattacagt tggcatattc
      481 gtatttgtaa tgcttatacc aattttaaaa tacaccatca gtactaatgg tattaaaata
      541 atctataata aagttaaaat tattccaatt agtacaatat tatttaatat tttacaactc
      601 atattctttt ttatataaat ttaccacctt atacattctt atttttagta tatattataa
      661 tggttttata tattatatca ataatttatt attgaaattc aataaattat atattataat
      721 caataatata ctttatataa aggagatgat attttatgat tacaaaattt tttagtaata
      781 tattatcagt atttctatat tttctattat cacttttagg agttgctggt ataatattct
      841 tatgtatgat acctatgttt ttatatgatt ttagttctga caaagtaatc gctttaattg
      901 caagaatact atatttttct gactacttta tagtcatctt attcttgata tttataatta
      961 atagtactaa gtattcccca ttcgtacttg aaaatgtaaa aaggtttaag gtaatgggat
     1021 gttgtttatt gataaacact ataattgaat gtataaatgg ttataattta aatactaata
     1081 atataagaat ttttggaact gatagtggcg gtatctcacc acttatggtt atctctttta
     1141 tatttgcact aatgtgcttt gtaattgcag aaacatttga taaagctata aaaataaaag
     1201 aagataatga tttaacaata tagaggtgat gttatggcaa tagtagttaa cctagatgta
     1261 atgatggcaa aaaggaaaat atcacttaaa gatttggcag aaaaaataga tattacaaat
     1321 gcaaatttat caattttaaa aactggaaaa gcaaaagcta ttagatttac cactctaaac
     1381 gaaatttgca aagctttaga ctgtcagcca ggtgatatac ttgaatatgt agaagatgaa
     1441 aatgattaaa aaagagtgtt tagaaacact cttttttatt atactatatg atttttattc
     1501 ttttactata ttattgacat taatattaac tttgtttaaa gtcataacaa aatatgttct
     1561 aagaaagaaa ctgttccttt atctatttgt tatccattac ctataagaac tgtatcacac
     1621 taaatcatac ttttaaattg ataattttac cacagattcc tatatacatt cttttctacc
     1681 taatatttgt ttaaactgtt agcaaaacat ggaacttttt catttactta tacctccatt
     1741 tatattattg catagtttat agctacgtgt tataaattac attattgatg attttatcta
     1801 acatttgtaa tcttttagat actctttgaa gttacaacac tgttatactc taattaagta
     1861 aactaaatct gatacaagtt ggtgattata tgaaaagaaa actttcaacc tattttaaag
     1921 gatatatttt aggtttatgt atagcatttt tattatttac ttttatatta aaaaaattaa
     1981 ttatagaaaa tatcattgga ataataattg gaactggaat tgcattttta ctgatttacc
     2041 tatttaataa aaaaagaaaa taatattcta taaaaaggtc ttttagtgaa tttacaaaaa
     2101 a
//
LOCUS       DAGSRM010000186         2031 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.192, whole genome shotgun sequence.
ACCESSION   DAGSRM010000186 DAGSRM010000000
VERSION     DAGSRM010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.192"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            205..1344
                     /locus_tag="MJE33_003608"
     CDS             205..1344
                     /locus_tag="MJE33_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBY9649513.1"
                     /translation="MVIKLTIEEYKNKYFTGNKIVLSLNSFDNKELYYHETENILNLI
                     NEIDKLTSSKIFKTIEDKNDLALESYYSSVIEGAFSTRKIAKSIIRGKMKPSNKSEYM
                     IYNNHRALEYGLDNLDKLYSHKFIYDLHHILGENCLDSEEYEYRTEKVYVCDSKGEII
                     HIGLEPLKIYDFMSKLIDFMENSKVSNLIKSAIIHFYFVYVHPFSDGNGRTSRALSYL
                     YLIDKGYDTFKEFSISYMISKNRTKYYKAILDVENKGNNLTVFIEFMLKSIIQSINEM
                     RNMHDRKSLESILKEELFENDITLSATEEHILKYICNKDNYSMTLENYIKKNKSRYLK
                     AGIKEIELVDQLMEVFSNLEEIEILSKEKNIYKVNDKYLKMLDID"
     gene            1370..1558
                     /locus_tag="MJE33_003609"
     CDS             1370..1558
                     /locus_tag="MJE33_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649514.1"
                     /translation="MNNKKKAFLISGAINVISLIIVFSLFHYNIIENSTQNMIITVIL
                     IFIFEMIKIKIIEKYYKL"
BASE COUNT          864 a          163 c          282 g          722 t
ORIGIN      
        1 atatataact aatgtattat aaataatagt tgtaaacttc ctgtaacata tattttaaat
       61 gcttattctt aaatttttgc attatttatt aaaatttatt atgctttaac gctaaattga
      121 gtttgataat tataaaacaa ataatcaaaa atagtgtaaa aattaatact ttttgttaat
      181 attaatagat aaagataatc taaggtggtg attaaactga caatagaaga atacaaaaac
      241 aaatacttta ctggaaataa aatcgtactt tctctaaata gttttgataa taaagaacta
      301 tactatcatg aaactgaaaa tatattaaat ttaataaatg aaatagataa attaacatca
      361 agtaagatat ttaaaacaat agaagataaa aatgatttgg cactagagtc ttattattct
      421 agtgtaatag aaggtgcttt ttcaactaga aagattgcaa aatctataat tagaggaaag
      481 atgaaaccta gtaataaaag tgaatatatg atttacaata atcatagagc cttagaatat
      541 ggattagaca acttagataa attatatagt cacaaattta tatatgattt gcatcatata
      601 ttgggcgaaa actgcttgga ttcagaagaa tatgagtata gaactgaaaa agtatatgta
      661 tgtgattcaa aaggtgagat tatacatata gggcttgaac ctttgaagat atatgacttt
      721 atgagtaaat taatagattt tatggaaaat agcaaagtaa gtaatttaat taaaagtgct
      781 ataattcatt tttactttgt atatgttcat ccttttagtg atggtaatgg aagaacatca
      841 agagccttat catatctata tttgattgat aaaggatatg atactttcaa ggagttttca
      901 atatcatata tgatatctaa aaacagaact aagtattata aggctatact tgatgtagaa
      961 aacaaaggaa ataatttaac tgtatttata gagtttatgc tcaagtctat aatacaatct
     1021 attaatgaga tgcgtaatat gcatgataga aaatctttag aaagtatatt aaaagaagaa
     1081 ttgtttgaaa atgacataac tctatcagca acagaagaac atatattaaa atatatttgc
     1141 aataaagata actattctat gacattagaa aattatataa aaaagaataa aagtagatac
     1201 ttaaaggctg gaataaagga aattgaatta gttgaccagt tgatggaagt gtttagtaat
     1261 cttgaagaga tagagatttt gtctaaagaa aaaaatatat ataaagttaa tgataagtac
     1321 ttaaaaatgc ttgatataga ctaatttaga taatctggag agggaatata tgaataataa
     1381 gaaaaaggca tttttgattt ctggggctat aaacgtaatt tcattaatta ttgttttttc
     1441 attgtttcat tataatatta tagaaaatag tacacaaaat atgataataa ctgtaatctt
     1501 aatttttatt tttgaaatga taaaaataaa aatcatagag aaatactata aactataaac
     1561 aagaaatatc ttttctaaga gcatattgta caatatgctc ttaatttagt tatatagatt
     1621 taatataaat aatgtatttt ttgtagatga tttatgtaag atttatgacc aatttgtgta
     1681 tgatttatgt tctttaatca tgacaatatt atattgtaaa aaataaattt aataaaaaat
     1741 gacttggaat ttatattcta ggtctttttt atataaaaat ttaataagtt tcaaatgttg
     1801 gtaactttga cagtagaaaa cgtctattga gataacgagg attctaattt agggggagat
     1861 atattagcac ttaaagtagt atatgcaaca gatataatct ttataaaagt aattctaatt
     1921 aaaatgtaaa tataaagaat aattaaaaaa atatcgcaat tatttgtttt tcatatggat
     1981 aaaattggtt atttaatata taattacaag tataaataat aaaatttatt t
//
LOCUS       DAGSRM010000187         1964 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.193, whole genome shotgun sequence.
ACCESSION   DAGSRM010000187 DAGSRM010000000
VERSION     DAGSRM010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.193"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            85..1374
                     /locus_tag="MJE33_003610"
     CDS             85..1374
                     /locus_tag="MJE33_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBY9649515.1"
                     /translation="MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYE
                     SGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKNGE
                     SFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKNSYRIFLEKDESQGN
                     YDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFLTENNVLMT
                     FVKIIAGILVLLVLFIIASNIKKKKRKKARSKKNMKK"
     gene            complement(1501..1839)
                     /locus_tag="MJE33_003611"
     CDS             complement(1501..1839)
                     /locus_tag="MJE33_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HBY9649516.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGRP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
BASE COUNT          714 a          254 c          270 g          726 t
ORIGIN      
        1 ttcatatatt aatcactcct ttcttttaat tattgacaaa tatatgctat actaaaagat
       61 gaatttaaaa tttggaggct cacaatgaaa agaaatttat cacttttatt aatctgttta
      121 ttgatattta cttcattttt aggaagctct aatatctctt ttgcagataa tgaaccagct
      181 atagttgcta aacatgcagt tttaatggac tatgagtctg gcaaaatact atataataag
      241 gatggaaatt ctaagcttta tccagcttca acaactaaag tatggacagc ttgtttagtt
      301 ctaaaggagg ttaaagattt aaatcaagta atagaaatta aagatttacc tcaaatagat
      361 ggttctagta tgtatctaaa aaatggggaa tcttttactg taaagcagtt actagatgct
      421 cttttggttc attctgctaa tgatgctgct tttgtattag ctagatatgt tggtggtggc
      481 aatgtacaaa aatttattga ccttatgaac tctgaggcaa aaaaaattgg agctactaat
      541 actcatttta ataatcctca tggattacca gacccaaatc actacacaac agctcatgat
      601 atggctctca tagctagaga agctatgaat aatgatactt ttagacaaat tgtaaaaact
      661 aagtcactta aatttgaagc tacaaaagca tatccttatg agagatactt tgtaaacact
      721 aataagtttt tgacatcaca tgataagatt acatacaaag gtcaacctat aaatataaaa
      781 tatgatatag ttgatggtat aaaaactgga tatactgatg ctgctggtaa atgtctttta
      841 tcgagtgctg taaaagatgg cagaagagtt atcgttgctg tatttaattc aactaatgct
      901 gatttatatt tagactcacg tattctgatt gattatggat ttgataactt taagtgtgct
      961 actattgttg ataaagaaaa atacacagat actaaaaaag tattgtttac aaaacaacat
     1021 gaattgattt atgaaccaaa aaatagttat aggatattct tagaaaaaga tgaatctcaa
     1081 ggcaattatg ataccaaaac agagttaaat aaaattgatt tgcctataaa aaaaggggct
     1141 aaggttggta ctttaaatgt atataataat ggtaaactag aaaacagtat agacttaatt
     1201 gcaaaaaata atcttgatag cagtcttcca tttttgactg aaaataatgt tttaatgact
     1261 tttgttaaga ttattgcagg tattctagtg cttttagttt tatttataat agcttcaaat
     1321 ataaagaaga aaaaaagaaa aaaagctaga agtaaaaaaa atatgaaaaa gtaacaagta
     1381 gttggaaatt ataataaaaa atttatacta tacttatcct tataaatcta aaagtactga
     1441 gataataatt ataactatta tctcagtact tttaatttta tgtttatgta ttttttatac
     1501 ctatatattt ttttgttttt gtttgtcttt aagcttacat tttgaacaat ttcccagaca
     1561 ttcatcaacc atatctagac attcattaaa tgctacctta ctactatttt tatgccttac
     1621 gattaatata tcttgttttt tagcttgaga ctcaaccgtt ctagacatct tatgtgaaac
     1681 agttgatgta agtatgacaa ctgcatctgg tcttcctata gaattattta atttagatga
     1741 catggtagta tatacctttg tcttataacc ttttttcttg gccataacca tataatcttt
     1801 ttccattctt tcatgaccac caatcactaa taagttcatt agatttcctc ccatttattt
     1861 gatattgatt ttcattatta tttatattat atataatagt atttagaaat aattttgtca
     1921 atacttattg atattaatta tcattttaat aataattttt tatt
//
LOCUS       DAGSRM010000188         1954 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.194, whole genome shotgun sequence.
ACCESSION   DAGSRM010000188 DAGSRM010000000
VERSION     DAGSRM010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.194"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            236..970
                     /locus_tag="MJE33_003612"
     CDS             236..970
                     /locus_tag="MJE33_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBY9649517.1"
                     /translation="MNLAKGLDVLKISSNVFGEDKVMYIPVIYTEDDATLIDTGLPGQ
                     GDLIIDALNKSNTSFDRLKNIIITHHDIDHIGNINYLREKSKNNIKVYAYKSEISYIT
                     GEETPFKLYMLEQMVDKIDDKMLSMLNVMGLGFKSSYTKVDVSLDNHEKLNLGEEIEV
                     IHTGGHTRGHICLYLKESKVLIAGDLLQVENGELKPVDVMYSNKQELKDAIKNISNYD
                     IETIVFSHGGLYQRNIIGTLKNLIIE"
BASE COUNT          884 a          168 c          280 g          622 t
ORIGIN      
        1 aaatagttat tagaaaaaaa taaaagtaat tgcaaagctt gcaattactt acttctatat
       61 taattactat accataaaat aacaaaaaaa tcaagtcgag taatccttca acttaaatga
      121 tatactaggt attaataatt tttaattaat aaaatataac tagaggaaaa tctacatttt
      181 ctcaatttct ttaattaaat tgacctctaa ttaagtaatt ataaggagaa aaattatgaa
      241 tttagcaaag ggattagatg ttttaaaaat ttcatcaaat gtattcggtg aagataaagt
      301 tatgtatatt cctgtaatct acacagaaga tgatgcaaca cttattgaca caggtcttcc
      361 gggtcaaggt gatttaataa ttgatgctct taataaaagt aacacgagct ttgataggtt
      421 aaaaaatata ataattactc atcatgatat agaccatata ggaaacataa attatctaag
      481 agaaaaatct aagaataata tcaaagtata tgcatacaaa agtgaaatca gctatataac
      541 aggagaagaa actcctttta aactttatat gttggaacaa atggtagata aaattgatga
      601 taagatgctt agtatgctaa atgttatggg acttggattt aaatcatcat atactaaagt
      661 agatgtgtca ttagataacc atgaaaaatt gaatctaggt gaggaaatag aagttataca
      721 tacaggaggt cacactagag gtcacatatg tctttattta aaagagtcta aagtacttat
      781 tgctggagat ttgttacagg tagaaaatgg agaacttaag cctgttgatg tgatgtacag
      841 caacaaacaa gagttaaaag atgctattaa aaatataagt aattatgata tagaaacgat
      901 agtttttagt catggtggat tatatcaaag aaacataatt ggaacattga aaaacctaat
      961 tattgagtag tactatatga tttacaataa tttcttaaat tacttaaaat tatatctaaa
     1021 gatgtagaat caaaattaaa tataagtaat aagaattttg ctttaaaatt atacttaagt
     1081 gttaaggatg atacgtagta ttacaagtat tttagaatat aattttttat agtaatttta
     1141 aggttctaca atacttcagt tgaaaaatat ttaacagcat agtataatag gaaagaaaat
     1201 actttaaata aactttagca tacaaagtga agaaaaatat ttaaattata tataatattg
     1261 tagagtgagg taaaatattt aagttatata tcataatgca gagtgaagaa aaatatttag
     1321 ataatgtacc ataatacaaa gtgagtaaaa atatttaaat catgtatcat aatgcaaagc
     1381 gaagaaaaat atttaaataa tgtaccataa tgtagagtga gtaaaaatat ttaaataatg
     1441 tgttataata caaagcgaat aagattattt aaagcatgtg ttataataca aagcgaataa
     1501 gattatttaa agcatgtatt ataatataaa gtgagtaaaa atatttaaat catgtatcat
     1561 aatacaaagt aaagaagatt atttaaacca tatatcataa tgcaaagtga agaaaaatgt
     1621 ttaaattatg tatcataata taagcagagt gaagaaaaat atttaaataa tatatcataa
     1681 tgtagagtaa agaaaaaatt taaatcatgt atcataatgc aaagtgaaga aaaatgttta
     1741 aattatgtat tataatataa gcagagtgaa gaaaaaattt aaatcatgca tcataatata
     1801 aacagagtga agaaaaatat ttaaataata tatcataatg tagagtaaag aaaaaattta
     1861 aatcatgtat cataatataa gcagagtgaa gaaaaaattt aaatcatgca tcataatata
     1921 agtagagtga agaaaaatat ttaaataata tatc
//
LOCUS       DAGSRM010000189         1869 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.195, whole genome shotgun sequence.
ACCESSION   DAGSRM010000189 DAGSRM010000000
VERSION     DAGSRM010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.195"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            18..1760
                     /locus_tag="MJE33_003613"
     CDS             18..1760
                     /locus_tag="MJE33_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M3"
                     /protein_id="HBY9649518.1"
                     /translation="MVKSKKKIFVFLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFE
                     KMEYKRPNIQSICENINKCNDKLLTAKTAKEQLNLFNEVDKIYQDFYSTLTIAKIRNN
                     IDSSDEFYSKEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGNDTFNYLKNIG
                     KLNSKEVEGLLKKEQDLVVKYEDLLAKSTVSIDGIEVDFEEALSRPNLSPEEYVKIYS
                     DYLKKYNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQEEAKKFRQDVK
                     DYIVPLYREISSKPSDSSIYIKVYKNRSFRKFDKVLEDISPKLKESFDYMKKYDLYDY
                     SSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKMQ
                     PSIDVCEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQEIIY
                     KNPYMSLNDINKLFFDLEEEYGVSNNILREKNAPFWILVSHNFQVPFYYLSYGLASDV
                     SLQIWQLSQEDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQLPFQNGNLEEISSTLYD
                     YFGIDNPLELKNAS"
BASE COUNT          737 a          223 c          264 g          645 t
ORIGIN      
        1 atgattggag gatttttatg gttaaatcca agaagaaaat atttgttttt ctaattttaa
       61 ttgcagtttt tattatattt gcatcataca taagaaatta tactaatgat acaaaaaaag
      121 attctgttag aggaactatt ccttttgaaa aaatggaata taagcgtcct aatatacagt
      181 caatttgcga aaacattaac aaatgtaacg acaaactttt aacagctaaa acagctaagg
      241 aacaattaaa tttatttaat gaagttgata aaatttatca ggatttttac agtacactta
      301 ctatagcaaa aattagaaat aacatagatt catcagacga attttactct aaggaatata
      361 agtatcttat gagtaaatca gtagatgttg acatgtcata caaagacttt caagatagat
      421 ttgtaacttc taaattctct aaagaattag aaaaagaact tggaaatgat acatttaatt
      481 atctaaagaa cattggaaaa cttaattcta aagaagttga aggtttgctt aaaaaagagc
      541 aagacctagt tgtaaaatat gaagacttat tagctaaatc tacagtatca atagatggta
      601 tagaggtaga ctttgaagaa gccttgtcaa gaccaaatct tagccctgaa gaatatgtga
      661 aaatttattc agattactta aaaaaataca atcctatatt tggtaacata ttcttagaac
      721 ttatacagac aagaactgaa atagcaagta agcaaggatt taagaattat atagattatg
      781 cctatatgaa cctaaacaaa gactattctc aagaagaagc taaaaaattt agacaagatg
      841 taaaagatta catagttcca ttatatagag agatttcatc taaaccatct gattcttcta
      901 tttatattaa agtatataaa aatcgctcat ttaggaagtt tgataaagta cttgaagata
      961 tatctccaaa actaaaagag agcttcgact atatgaaaaa atatgacttg tacgattatt
     1021 cttctggaaa aaacaaatca cctggaggat atactactta tataaataaa tataaagcac
     1081 ctttcttgtt taatacatgg gacaattctt ttttaggtgt tactagtttt gctcatgagt
     1141 ttggtcattt ttataattac tacaacagta ttaacttaaa taataagatg caaccaagta
     1201 tagatgtatg tgaagttcat tcaacatctt tagaaattct attttataaa tattttgatg
     1261 atttttttgg taaacaatct gaagctataa aaaaagaaca cttatcgatt gttttaaata
     1321 ctattattga tgcttgtctt tatgatgaat ttcaagaaat tatatacaag aatccatata
     1381 tgagtctaaa tgatatcaat aaattgtttt ttgatttaga ggaagaatat ggagtttcca
     1441 ataatatact tagagaaaaa aatgctcctt tttggatact tgtatctcat aacttccaag
     1501 ttccttttta ttacttgagt tatggtcttg catctgatgt atcacttcag atatggcaat
     1561 tatcacaaga agattacaga aaagctgttg atgtatatat ggatttttta aatcaaaata
     1621 ctgatgctgg atttaaagat gtggtagaaa aggtaaattt acaattacct ttccaaaatg
     1681 gaaatttaga ggaaataagc tctactttat atgattattt tggtatagat aatccacttg
     1741 aactaaaaaa tgcttcttaa tttctaatac tttatactca ctcttaagat ttagtattag
     1801 attgaaataa gtgatatttt ttcacacaca attaatgttt aataaaatta tatggtaaag
     1861 tatcagaaa
//
LOCUS       DAGSRM010000190         1824 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.196, whole genome shotgun sequence.
ACCESSION   DAGSRM010000190 DAGSRM010000000
VERSION     DAGSRM010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.196"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            40..1617
                     /locus_tag="MJE33_003614"
     CDS             40..1617
                     /locus_tag="MJE33_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HBY9649519.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLIGNGRYETAVK
                     ISQKAYSSSPNVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDDRTEKEIKRLGA
                     KDIYLIGGTAVLNKDIENKLKSNGLNVERINGKDRYETSLILANKLKDIKDIKEVAVV
                     NGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVIGGTNSVSR
                     AVEKSLPNAERVSGKDRNETNAKVIEKFYTDTNLSNLYVTKDGSKNENQLIDSLAVGV
                     LAAKNESPVVLVGNKLNTKQRDILSTKKLNTITQVGGNGNEEAFDEIKSLQEKTVFEA
                     KTVEELTDMINIASPNDIINFKPKENTVNEAFRMVTNKPITVNIKGDCSKTLTVDMPN
                     GEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDIDTLMILASA
                     NDTKLINDGYIGKLIDNSSNSDITNNGTIDKKVNQVEDLEAKVDSIEKAIDSISQKVN
                     KIQDILDKLGFLKKFLS"
BASE COUNT          753 a          178 c          355 g          538 t
ORIGIN      
        1 aatatagtaa gattttaaac aaaataaggg ggaataataa tgaaagcacc aaaaactatt
       61 ttaacaatac tgacaatagc gcttacttta agtagcattt ctataatacc atcatatgca
      121 cttacagagg aaaaattaat aggtaatggt agatatgaaa ctgctgtaaa gataagtcaa
      181 aaggcataca gttctagtcc taatgtagtg ctagttaatg acaattcatt agctgatgca
      241 ctttcagcta caccatttgc aaaggctaaa ggagcaccaa tacttttgac agaaagtgat
      301 aagctagatg ataggactga aaaagagata aaacgtctag gagctaaaga tatttatttg
      361 ataggaggta ctgctgtact taataaagat atagagaaca agttaaaaag taatggactt
      421 aatgtagaaa gaattaatgg taaggataga tatgaaactt cactgatttt ggctaataag
      481 cttaaggata taaaagatat taaggaagtt gctgtagtaa atggtgaaaa aggattgagt
      541 gatgctgtta gcgttggggc accagcagct caaaataaga tgcctataat tttgtctagt
      601 cctaaggatg gagtagaagc atttgataaa tttataaggg atgagaaagt tataaaagca
      661 tatgtaattg gaggtacaaa ttctgtttca agagctgtag aaaagagtct tcctaatgca
      721 gaaagagtga gtgggaaaga tagaaatgag actaatgcta aagttataga aaagttttac
      781 actgatacaa atttaagtaa tttatatgta actaaggatg gtagtaaaaa tgagaatcaa
      841 ctaatagact cgttagctgt tggcgtattg gctgcaaaaa atgagtctcc tgttgttttg
      901 gttggtaata aattaaacac aaaacaaaga gatatactta gtactaaaaa attaaatact
      961 ataacacagg taggtggaaa tggaaatgaa gaagcttttg atgaaattaa aagtctacaa
     1021 gaaaaaactg tgtttgaagc aaaaactgtt gaggaattaa ctgatatgat aaatatagca
     1081 agtcctaatg atattataaa ctttaaacca aaagagaata ctgtaaatga agcttttaga
     1141 atggtaacaa ataaaccaat aactgtgaat ataaagggtg attgttcaaa gactcttaca
     1201 gtagatatgc ctaatggtga agtgaataac tatgctactt tggtaaatgt tatagttaga
     1261 aatattggag aaggtggatt taataatcat gatacaatca ctatattgtc agttcgtgac
     1321 aaaaatggta gagttataga aaatactaga aattcagata tagatacgtt gatgatatta
     1381 gcttctgcta atgatacaaa attgattaat gatggatata taggaaaact tatagataat
     1441 tcatctaata gtgatataac taataatggt actatagata aaaaagttaa tcaggttgaa
     1501 gatttagagg ctaaagtaga ttcaattgaa aaagctatag attctataag tcaaaaagtc
     1561 aataaaatac aagatatact agataagctt ggatttctaa agaaatttct tagctaagaa
     1621 tatgtatatt taagtgaagt gtgtgttgta attgttctaa agttatgtag ttaaacaggt
     1681 ttatatacaa atagatttat agataaatat gtagtttagt atatatttga ataagctttg
     1741 gggaggtaac atggggattg gaattgaatg taacaaaaaa ataaataaaa gtattgacaa
     1801 aactagaata aagaaaaata atct
//
LOCUS       DAGSRM010000191         1598 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.197, whole genome shotgun sequence.
ACCESSION   DAGSRM010000191 DAGSRM010000000
VERSION     DAGSRM010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.197"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(227..538)
                     /locus_tag="MJE33_003615"
     CDS             complement(227..538)
                     /locus_tag="MJE33_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649520.1"
                     /translation="MKKIIKSFTFWFFLIALFEIYMHQIGQDSKSIVLIGLNPILSII
                     SRVDSFFVFMDSGMQIPCRTITGSISIYWYIASILSFLIYGIILDLIRIVISKIGNKT
                     K"
     gene            complement(594..1187)
                     /locus_tag="MJE33_003616"
     CDS             complement(594..1187)
                     /locus_tag="MJE33_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBY9649521.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEINDNGLLPGSRQHIAGANYTVEIMKQAPVTIFIL
                     NILGKSPLEKLSPEERFYEMANMQSIGAAIQNMSLTAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLSDVTEWR"
BASE COUNT          587 a          206 c          201 g          604 t
ORIGIN      
        1 aaattaatct aaagaagtat aataaactaa tttaagaaat atagaaaatt aatttaaaga
       61 aatacaatac attaattttt aagtaacatt aaattaatgt aagtaaaata atgttcatat
      121 gaagatttaa aggtgcattt tctgaagcta agtatatgtt gtagagtgtt aggtctctct
      181 atcctgtgaa gtttaatagc tcaagaaaat gcactattta tgatgattat tttgttttat
      241 ttccaatttt ggaaataact attctaatta aatctaaaat tataccataa ataagaaaag
      301 aaagtataga tgctatgtac caataaatcg aaattgaccc tgtaattgtt ctacatggga
      361 tttgcatacc tgaatccata aaaacaaaaa aactatcaac tctggaaata atagacaaaa
      421 taggatttaa acctattaga acaatacttt ttgagtcttg accaatttga tgcatataaa
      481 tctcaaataa tgcaattaaa aaaaaccaaa atgtaaaaga tttaataatt tttttcatca
      541 aaatacctca cataatataa attctgtttg cactatttgc ttactacaac tatttatctc
      601 cattcagtca catcacttaa ttgtaatcta ggtcttcttg atggttcttc atcaggataa
      661 cccaaagata ttgctgccac taattgacta tctgtattta accattcaca taattcacga
      721 tatgcaaagt atacatcaca aatccaaaga ctacctagac ctaattccac agctgtaagc
      781 gacatattct gaattgcagc tccaattgat tgcatgtttg ccatttcata gaatcgttct
      841 tcaggggata gcttttctaa aggtgattta cctaatatat ttaaaataaa aattgttact
      901 ggagcttgtt tcatgatttc aactgtgtaa tttgcaccag cgatatgttg acgacttcct
      961 ggaagaagcc cattatcatt aatttcattt tgaatacctt tgctcatggc tttaagcata
     1021 gattcttttt ccttttcagt gatgacaaca aaacgccatg gttgcctgtt tttagatgaa
     1081 ggtgcattta ttccagcttc aattattttc tctatagttt catgtgaaat gctttgattt
     1141 ttatattttc tgatacttcg tctttttgat atagaatcac ttatcatttt aagtaacctc
     1201 cgtttatttt ttggaaaaat ctatttaatt tttattttct attttaatta ttttagcatt
     1261 aatcagaaaa aaagtcattg attttagtat acaattatta atgattatat taagaattgt
     1321 atcaaaaagt atgtgattat actaagggat tatattaaac attatattta aaattgtatg
     1381 aaaggttgta tttaatatta tattgagaga tatatatatt aggatttata tatattcaga
     1441 tttattatta tattgagcct tatattaaat taaggactat aatttgaaaa taagctataa
     1501 aaacttaata ttgttttgtt cagagataaa aaaagaatac tttaggtgat ttttattaaa
     1561 aattaagagt gataaaatta tatactttat aaatgtaa
//
LOCUS       DAGSRM010000192         1571 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.198, whole genome shotgun sequence.
ACCESSION   DAGSRM010000192 DAGSRM010000000
VERSION     DAGSRM010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.198"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(441..932)
                     /locus_tag="MJE33_003617"
     CDS             complement(441..932)
                     /locus_tag="MJE33_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HBY9649522.1"
                     /translation="MGMIGCYTKISEENVFKLQQAEENLQDFVFEDANENSTINIDKA
                     WHAIHFTLTGCPFGGDDDNIFSKLVLSGNILMEIDGEFPVMLITANDVKKLSKAMNSL
                     EEQAFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKKFIQEASNDGLAVIF
                     FIM"
BASE COUNT          599 a          192 c          135 g          645 t
ORIGIN      
        1 tattatttat aaatttctaa attttttcta aagattttaa tttttctgtt tttaattaaa
       61 ttaaagtatc tttgtttctg aaatttttta agtttatgaa gttataattt aatatatatt
      121 tattttattt gtgagtctat ttttatatat aatgaaaaat gtgataagta tccaaaagaa
      181 ttactggata taataagata gggtatttag ccttttaaga taaataaaaa tatctgcaac
      241 tatatattgc aaaaatttaa aactaattta tatatgctaa tataaatatt tataaagaca
      301 taagacaaat ttacctacag atttaaattt aacttatata ttttaaaagg ctaatataat
      361 atgtattttc ttctatacaa tcaaaaggct tcatatctaa gatatgtgat gacactaata
      421 ttcacaattt ttagtaatta tcacattata aaaaatatta cagctagtcc atcattagat
      481 gcttcttgta taaacttttt aagctcaatt agatttgccc atacatattc aaaaaaatca
      541 tcttcactct catcattcat aacaggataa atattatttt ctaacatctc attaatatta
      601 aatctttttc taaatgcttg ttcttccaaa ctgttcattg cttttgatag tttttttaca
      661 tcatttgctg taatcaacat gacaggaaat tctccatcaa tttccataag tatgtttcca
      721 cttagtacta atttgctaaa tatattatca tcatctccac caaaaggaca acctgtcaaa
      781 gtaaagtgaa tggcatgcca tgctttatca atattgatag tactattttc gttggcatct
      841 tcaaaaacaa aatcttgtaa attttcttct gcttgctgta atttaaaaac attctcttca
      901 cttattttag tataacatcc tatcatcccc ataattacat tcctcctaag tagcgttttt
      961 ttaattgtaa cttataatta aacttagtta caataaaata tattgagaaa agtattattt
     1021 catcttaaat ttaaatacct atattatcaa ctaaaattct gtgtttatag cgtaagtatt
     1081 attatttttt cctaaaaatc aaatgaatca gaagttctat aggcaatatt gactataaat
     1141 aagaactatt acttttaaaa aaaatttaag aaattatact gtagaatcat gctctactta
     1201 ttagcttcaa ataaaaaata aatcatgttc tcaaattaaa taataaataa caataaattt
     1261 aaattttaaa ctataagagt ttctgaagtg ttaataagca taccaataat tatatgtgta
     1321 agaatttgat ttaatagata tgaataattt ttacattaat tataaatata ttaacctatt
     1381 gttataaatt ctaaaaaaga aaagttatct taaataaaaa ataagtgtat cttaaatttt
     1441 aaatgataca cttattttta tattgaaatt atatttttta atatctattc ttaattaggt
     1501 atttttaaat tatctgttta cattaaatat ttcttaatta cttatctttc agctagcttt
     1561 ttctattaaa t
//
LOCUS       DAGSRM010000193         1381 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.199, whole genome shotgun sequence.
ACCESSION   DAGSRM010000193 DAGSRM010000000
VERSION     DAGSRM010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.199"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            101..1315
                     /gene="dpaL"
                     /locus_tag="MJE33_003618"
     CDS             101..1315
                     /gene="dpaL"
                     /locus_tag="MJE33_003618"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBY9649523.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLKDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKSLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLNTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAKEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAK
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNYSSAFVSAPDWVSAKGMRILGNP
                     LRGDEKVISGESGAVTTGALVSILESEDLKDLREALKLDENSKVLLISTEGDTDPDKY
                     RDIVWNGECQSK"
BASE COUNT          564 a          160 c          292 g          365 t
ORIGIN      
        1 ttagaataaa taatattggc acggtaattg ctttgctata aggcacaaag ataaaaaatt
       61 aaaatgacac cgaaaaattt gaaaggggaa tataaaataa atgagagaaa taaaatggaa
      121 attaaataac ttacctaaat cagaggaaaa agaaaaagga attgaatttt taaaagatga
      181 agagattgca aaggctaaag cttttcatga aagctttcca caatatgaaa agactccttt
      241 agttaaatta gataacttag caaaatcact aggcgtaagc ggaatatatg ttaaagacga
      301 atcttataga tttggtttaa atgcatttaa agttttaggt ggttcttact ctatgggtag
      361 atacttagct caaagattaa atacagatat atctgagtta gggtatgata aattaacttc
      421 aaaagaaata aaagaaaaat taggagaaat aactttcttt acagccacag atggaaatca
      481 tggtagagga gttgcttgga cagctaataa attaggtcaa aaatctgttg tattaatgcc
      541 aaaaggttct tctgaattta gattaaataa aatcaaagga gaaggtgcag atgctagtat
      601 aacagactta aactatgatg atgcagtaag attagctaat gattatgcag aagctgatga
      661 ccatggagta atggttcaag atacagcatg ggacggatat gaagaaatac cagcatggat
      721 aatgcaagga tatggaacta tggctaaaga agctatagaa caattaaaag aatatggagt
      781 agatagacca acacacgtat ttgttcaagc aggagttggt tcacttgctg gtgcagttca
      841 agggtatgtt gcttcaatat atgatgaatg cccaataaca gtagtagtag aggctgatga
      901 ggctgattgt tactataaat cagcaaaagc tggagatgga aaaccaagat ttgttggagg
      961 agatatgcca actataatgg cagggcttgc ttgtggagaa ccaaacacta taggttttga
     1021 agtgttaaaa aattattcat cagcattcgt ttcagcacca gactgggtat ctgctaaagg
     1081 tatgagaata ttaggaaacc ctctaagagg agatgagaaa gtaatctctg gagaatctgg
     1141 agcagttaca actggagcat tagtttcaat ccttgaaagt gaagatttaa aagacttaag
     1201 agaagctttg aaactagatg agaactctaa agtattatta ataagtacag aaggagatac
     1261 tgaccctgat aaatatagag atatagtttg gaatggtgaa tgccaaagta aataaaagac
     1321 ttaaggaaaa gaaaaactta ataaataaat gaaaaagtaa agcaaataaa aaagtaaagc
     1381 a
//
LOCUS       DAGSRM010000194         1339 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.200, whole genome shotgun sequence.
ACCESSION   DAGSRM010000194 DAGSRM010000000
VERSION     DAGSRM010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.200"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            72..156
                     /locus_tag="MJE33_003619"
     tRNA            72..156
                     /locus_tag="MJE33_003619"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:106..108,aa:Tyr,seq:gta)
     gene            166..239
                     /locus_tag="MJE33_003620"
     tRNA            166..239
                     /locus_tag="MJE33_003620"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:198..200,aa:Gly,seq:tcc)
     gene            250..326
                     /locus_tag="MJE33_003621"
     tRNA            250..326
                     /locus_tag="MJE33_003621"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:284..286,aa:Arg,seq:tct)
     gene            336..411
                     /locus_tag="MJE33_003622"
     tRNA            336..411
                     /locus_tag="MJE33_003622"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:369..371,aa:Gln,seq:ttg)
     gene            423..498
                     /locus_tag="MJE33_003623"
     tRNA            423..498
                     /locus_tag="MJE33_003623"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:456..458,aa:Lys,seq:ttt)
     gene            501..589
                     /locus_tag="MJE33_003624"
     tRNA            501..589
                     /locus_tag="MJE33_003624"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:535..537,aa:Ser,seq:tga)
     gene            607..697
                     /locus_tag="MJE33_003625"
     tRNA            607..697
                     /locus_tag="MJE33_003625"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:641..643,aa:Ser,seq:gct)
     gene            706..782
                     /locus_tag="MJE33_003626"
     tRNA            706..782
                     /locus_tag="MJE33_003626"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:740..742,aa:Pro,seq:tgg)
     gene            868..943
                     /locus_tag="MJE33_003627"
     tRNA            868..943
                     /locus_tag="MJE33_003627"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:901..903,aa:Trp,seq:cca)
     gene            1004..1080
                     /locus_tag="MJE33_003628"
     tRNA            1004..1080
                     /locus_tag="MJE33_003628"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1038..1040,aa:Pro,seq:tgg)
     gene            1087..1163
                     /locus_tag="MJE33_003629"
     tRNA            1087..1163
                     /locus_tag="MJE33_003629"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1121..1123,aa:Ile,seq:gat)
     gene            1167..1242
                     /locus_tag="MJE33_003630"
     tRNA            1167..1242
                     /locus_tag="MJE33_003630"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1200..1202,aa:Phe,seq:gaa)
     gene            1250..1326
                     /locus_tag="MJE33_003631"
     tRNA            1250..1326
                     /locus_tag="MJE33_003631"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1284..1286,aa:Met,seq:cat)
BASE COUNT          320 a          318 c          371 g          330 t
ORIGIN      
        1 tagagcagct gacttgtaat cagcaggttg taggttcgat tcctatcacc agctccatgt
       61 aaaactaata cggaggattt cccgagcggc caaagggggc agactgtaaa tctgttgtca
      121 tcgacttcgg tggttcgaat ccaccatcct ccaccaatta aatatgcggg tgtagctcaa
      181 tggtagagtt ctggccttcc aagccagctg tgagggttcg atccccttca cccgctccaa
      241 agaaagcgtg ggtgcatagc tcagctggat agagcaacgc ccttctaagg cgtgtgtccg
      301 gggttcgaat ccctgtgcgc tcaccacata tatattgggg attagccaag tcggtaaggc
      361 acatgacttt gactcatgta tgcgtaggtt cgagtcctgc atccccagcc aaataaaaat
      421 atggtctatt agctcagtcg gtagagcacc tgacttttaa tcagggtgtc ccgcgttcga
      481 gtcgcggata gatcaccaat ggagaggtgt ccgagtggtt taaggagctg gtcttgaaaa
      541 ccagtgactt cgaaaggggc cgtgggttcg aatcccaccc tctccgccaa aaaaatgact
      601 gaatttggag aagtactcaa gtggctcaag aggatcccct gctaagggat taggctgggt
      661 aaccggtgcg agggttcgaa tccctccttc tccgccattc aaaaacgagg tgtagcgcag
      721 tttggtagcg cacatggttt gggaccatgg ggccgggggt tcgagtccct tcacctcgac
      781 caacttaagc ttttagttca gttggttaga gtgcccaact catggctagt aattctaggg
      841 tttgagtcca tgaaggttat tcttcatagg ggtgtagctc agttggtaga gcgtcggtct
      901 ccaaaaccgt gcgccggggg ttcgagtccc tccacccctg ccaaataaaa aggacttgaa
      961 aaaagttgga ttttagagta gtataagata gtaaataaga tttcgaggtg tagcgcagtt
     1021 tggtagcgca catggtttgg gaccatgggg ccgggggttc gagtcccttc acctcgacca
     1081 agaaaagggc ctatagctca ggtggttaga gcgcacgcct gataagcgtg aggtcgctgg
     1141 ttcgagtcca gctaggccca ccatttgccc agatagctca gtcggtagag caggggactg
     1201 aaaatccccg tgtcggtggt tcgattccgc ctctgggcac caggaatacg gcggtatagc
     1261 ttagttggct agagcgttcg gttcataccc gaaaggtcat aggttcgact cctattaccg
     1321 ctaccatcta actatatca
//
LOCUS       DAGSRM010000195         1118 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.201, whole genome shotgun sequence.
ACCESSION   DAGSRM010000195 DAGSRM010000000
VERSION     DAGSRM010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.201"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(269..985)
                     /gene="dapD"
                     /locus_tag="MJE33_003632"
     CDS             complement(269..985)
                     /gene="dapD"
                     /locus_tag="MJE33_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBY9649524.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     SNGSYIFVGDYEDIMEVLDKHGNDITDTHVEYDRRNSAIPLKNTLSEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
BASE COUNT          393 a          190 c          124 g          411 t
ORIGIN      
        1 cactttatta aattataaaa agtttaatag tttattacat tgatatattg aaaaattgaa
       61 ggaaataaaa atacttgtca gcaagaaata aaaatgatat aagaatgaat aagagaattg
      121 taaaaaaata aaatgattat aaataaaaga taaaaatatt gtaaaaaaga gggtgcctct
      181 ggtgaattaa attcacacag agacaccttt ttaattaatt ttttaaatta gtatattatt
      241 gtttaattaa tagcatagta ctatttaatt agtctaaatt tctaaggtct tccataagct
      301 tagttttgtc agccgttttt tcatctttca tttttataac tttagcagga gaaccagcaa
      361 caacggcacc agcttctaca tcagtagtta caacagcacc agctgcaaca acagcacctt
      421 taccgattct aacaccttca agaataactg cattagctcc tattaaaaca tcatcttcaa
      481 ctataacagg agtagcagaa ggtggctcta aaacaccagc aacaacagca ccagcaccta
      541 agtgaacatt tttaccaaga gttcctctag ctccaataac agcattcata tctaccatag
      601 aaccttcacc aataacagct cctatattta taacagctcc catcattaca acagcatttt
      661 tttctatagt aaccatgtct cttattattg cacctggctc aattcttgca tgttcactta
      721 aagtattttt tagaggaata gcagaatttc ttctatcata ttctacatga gtatctgtta
      781 tatcatttcc atgtttatct aatacttcca ttatatcctc atagtcacct acaaatatat
      841 aagaaccatt acttccaaat accttaaatt tatcagttga ttggaagtgt aaagagttag
      901 tatttacata taccttaaca ggggttttct ttttaacttc ttttatatac cttgctattt
      961 cataagcatc gtttaaattt accatttgtt aattctcctt gtatttcatt atatttcttt
     1021 tattatatac ttatttgtca attttagtat atacttactt cttattattt atattttatt
     1081 tagtttttat tgtatagaca tagtattaaa taaattta
//
LOCUS       DAGSRM010000196         1116 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.202, whole genome shotgun sequence.
ACCESSION   DAGSRM010000196 DAGSRM010000000
VERSION     DAGSRM010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.202"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(192..1061)
                     /locus_tag="MJE33_003633"
     CDS             complement(192..1061)
                     /locus_tag="MJE33_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBY9649525.1"
                     /translation="MVIIKAIELYKKLDLEFNIKNINDDWSFMNFENDFITPEFRKKY
                     IGLVLDNAQNINKVYTTTFPDKEIIKQVIDKDEKDILIFSHHAMGYIASDEGFPFHDI
                     PLSYMEEMKNRRISFYVLHSPLDNYSDYSTSVSFAKALGLEIVKPFCKYDDKIEVGVI
                     CKTSLKSIEEIKELIKKSVGHDVKLYDYGDSVLKDGLVAIAAGGGSYPFVAREVAELG
                     INLYITGFTKPLKHFEPVLEFHQIAKDNLINVIGATHYSTEKFACMGMVDYFKNLGLE
                     SEFLQGKYYLEDL"
BASE COUNT          418 a          172 c          108 g          418 t
ORIGIN      
        1 aaactaaaaa atatcgaaaa ataatttaaa agacatcaaa actaaatctt ctaatcagca
       61 attaaaagtc tattttctta gtttaaaaaa taaagataga ttttttaaaa acactggata
      121 aataggtaaa aaaataaaca gttagtaaaa taaattttta ctaactgttt atttttattg
      181 tttaatatgt cctataaatc ctctaagtaa tactttcctt gcaaaaattc tgattctaaa
      241 cctagatttt taaaataatc aaccatgccc atacacgcaa atttttctgt agaataatga
      301 gtagcaccta taacattaat taaattatct tttgctattt gatggaactc taatactggt
      361 tcaaaatgtt ttaaaggctt agtaaaccca gtaatatata gatttatacc aagttcagca
      421 acttctcttg ctacaaatgg ataacttcct ccccctgctg caatagcaac taaaccatct
      481 tttaatacag aatctccata atcatacaat ttcacatcat gaccaacaga ttttttaatt
      541 aattccttaa tctcttctat tgattttagt gatgttttac atataactcc tacctcaatt
      601 ttatcgtcat atttacaaaa tggtttgact atttctaaac ccagagcttt tgcaaaacta
      661 acagaagttg aatagtcact atagttgtct agtggagaat gtaatacata aaaagaaatt
      721 ctacgatttt tcatctcttc catataagat agtggtatgt cgtgaaatgg aaaaccttca
      781 tctgatgcta tgtatcccat tgcatgatgt gaaaaaatta aaatatcttt ttcatcttta
      841 tctataactt gcttaattat ttctttatca ggaaaagtag ttgtgtatac tttattaata
      901 ttttgagcat tatctaatac taatcctatg tatttttttc taaattctgg agtaataaaa
      961 tcattttcaa aattcataaa actccaatca tcattaatat ttttaatatt aaattctaaa
     1021 tctaattttt tataaagttc aattgcctta ataataacca ctcctttata ataatattga
     1081 attactacat attaatatag aaatttccga taataa
//
LOCUS       DAGSRM010000197         1103 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.203, whole genome shotgun sequence.
ACCESSION   DAGSRM010000197 DAGSRM010000000
VERSION     DAGSRM010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.203"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            <1..1024
                     /locus_tag="MJE33_003634"
     CDS             <1..1024
                     /locus_tag="MJE33_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBY9649526.1"
                     /translation="NTINLMKKYLDIPSPAGYTENAVLEIKKDFENLGLDTILTKKGA
                     LIATLPGEDDSNQITISAHMDTLGAIVKEISSDGTLKYHKVGGGCWAAIEGENCTVIT
                     RKGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLGISVGDF
                     VCLDHRFEATESGFIKSRYIDNKSAVAMVLEICRYFKENNLTPKHTTNFYISNYEEIG
                     HGVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKLVDVAED
                     NNLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIENTLKLLI
                     NYMLKE"
BASE COUNT          429 a          136 c          186 g          352 t
ORIGIN      
        1 taatactata aatttaatga aaaaatattt ggacattcca agcccagctg gatatactga
       61 aaatgcagtt ttagaaatca aaaaagattt tgaaaattta ggtcttgaca ctattcttac
      121 aaaaaaaggt gcacttatag ctactcttcc tggtgaagat gattcaaatc aaataacaat
      181 atctgcacat atggatacac ttggagctat agtaaaagaa atatcatctg atggtactct
      241 taaataccat aaagttggtg gtggatgttg ggcggctata gagggagaaa actgtactgt
      301 tataactaga aaaggtaaaa aaatcagagg aagtgttgta tttaagtatg cttctactca
      361 tatatatggt caaacaaaag ctgccactga aagaaatgaa gaaacaatga ttattagact
      421 tgatgaaaaa gtatttacaa agcaagatgt attagattta ggaataagtg ttggggattt
      481 tgtatgccta gaccatagat ttgaggctac agaatctggt tttataaaat ctagatatat
      541 agataataaa tcagctgtag caatggttct tgaaatatgt agatatttta aagaaaacaa
      601 tttaacacct aagcatacta ctaactttta tataagtaat tatgaagaaa ttggacatgg
      661 tgtttctaaa tgtttacctg aaaaaacaaa agaatttgta gcaatagata tagcacctac
      721 tggaccaagt caaacatcta gtgaacatgg tgtaactata gttgcaaaag ataatttatc
      781 attatatgat tttaatttaa gaaacaagct tgtcgatgtt gcagaagaca ataatttaga
      841 ctacgctgtc gatgtattta ctttctatgg ttcagatgct tcagaagcta ttcgttgggg
      901 agaagatgtt caaatagcat gtgttggtcc tggaacaaac aatagtcacc attatgaaag
      961 aacacatata gaagctattg aaaatacact aaaattactt ataaattata tgcttaaaga
     1021 ataattaatt atatatttta ccaaagtcaa ttcttaacaa ataattaact ttaattttta
     1081 tttttataca aaaattggtt ttt
//
LOCUS       DAGSRM010000198         1043 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.204, whole genome shotgun sequence.
ACCESSION   DAGSRM010000198 DAGSRM010000000
VERSION     DAGSRM010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.204"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(137..820)
                     /locus_tag="MJE33_003635"
     CDS             complement(137..820)
                     /locus_tag="MJE33_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBY9649527.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKTKIHWRQVIIIIEMI
                     TILIVSFIPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAIIIFFISGAILGTFFTKIFVEKSILICFFILAVVFGIMFI
                     NKDDSFNEN"
BASE COUNT          406 a          160 c           78 g          399 t
ORIGIN      
        1 ttactatact taaaatactt ctattaactt atacattgct tactatattt taaattcttt
       61 aattaatcta tacaatattt gctatatata aagtatatca aataacagct tcattttttt
      121 gtaaatttac agaaagttaa ttttcattaa atgaatcatc tttattgata aacataattc
      181 caaagaccac tgccaaaata aaaaaacata ttagtattga tttttccacg aaaatttttg
      241 taaaaaaagt tcctaaaata gctccagata taaagaatat aataatagca taatactgta
      301 aactcttttt tatcataaaa atattcttag tatgtatata atgaaataaa gtttctgtag
      361 cacttcttaa atttccagta cacattgtcg ttgcaaaact attcccattt acttttctaa
      421 aactctctac ttgcattgaa caaataaaag aaatagcaac atttacaaac atattaaatc
      481 ttcctctagg aataaatgaa acaataagta ttgtaatcat ttcaattata atgataacct
      541 gacgccaatg aatctttgtc ttattatttg taaaatactt tcttatcttc tcggctataa
      601 atacacctaa cataaatgcc aaaataggaa tcaaataata aaatgattcc tttacattac
      661 cttctgctat attaagccca aacaaaacta tatttccagt ttgtgcatta gcaaagactt
      721 tacctcttac cacataagta taaatatcca aaaatcctcc tgttattgcc aacaaagaac
      781 ataaaaagaa tgattcagac atctgactct tgcttgtcat atataaacct ctcgctttct
      841 aatgatagat acaaaatcaa aatcatttta gattctattt ttcattgata taaaaaaatt
      901 atttatattt aaatattgtc tatatttata catcatttat atttatacat tatttgtatt
      961 tatatagcat tcatatttat ttatatttaa acattatcta tatctatata gcatttatat
     1021 ttatttacat ttaaacatta tct
//
LOCUS       DAGSRM010000199          988 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.205, whole genome shotgun sequence.
ACCESSION   DAGSRM010000199 DAGSRM010000000
VERSION     DAGSRM010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.205"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..>988)
                     /locus_tag="MJE33_003636"
     rRNA            complement(<1..>988)
                     /locus_tag="MJE33_003636"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          220 a          291 c          223 g          254 t
ORIGIN      
        1 ctattagtat cgataagcta aatacattgc tgcacttaca ccttcgacct atcaaccaga
       61 tagtctttct ggggtcttac ccttgcggtg ggaaatctta tcttgaagtt ggcttcgcgc
      121 ttagatgctt tcagcgctta tccattccgt acatagctac ccagccatgc ccttggcagg
      181 acaactggta caccagaggt acgtccatcc cggtcctctc gtactaagga caggtcttct
      241 caaatttcct acgcctgcga cggataggga ccgaactgtc tcacgacgtt ctgaacccag
      301 ctcgcgtacc actttaatgg gcgaacagcc caacccttgg gacctactac agccccagga
      361 tgtgatgagc cgacatcgag gtgccaaacc tccccgtcga tgtggactct tgggggagat
      421 aagcctgtta tccccagggt agcttttatc cgttgagcga tggcccttcc acgcagaacc
      481 accggatcac taagtccgat tttcatcctt gctcgacctg tatgtcttgc aatcaagctc
      541 tcttttgcct ttacactcta cgtacgattt ccgaccgtac tgagagaacc tttgagcgcc
      601 tccgttacct tttgggaggc gaccgcccca gtcaaactgc ccacctgaca gtgtcccaag
      661 accagattca tggcctatgg ttagagtccc agtactacaa gggtggtatc ccaaggatgg
      721 ctccgccaaa actggcgtcc tggtttcaaa gcctcccacc tatcctgtac atgtagtacc
      781 aagacccaat gtcaagctac agtaaagctc catggggtct ttccgtcctg tcgcaggtat
      841 ccggcatctt caccggaatt acaatttcac cgagtctgtt gttgagacag tgcccaaatc
      901 gttacgcctt tcgtgcgggt cggaacttac ccgacaagga atttcgctac cttaggaccg
      961 ttatagttac ggccgccgtt tactgggg
//
LOCUS       DAGSRM010000200          962 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.206, whole genome shotgun sequence.
ACCESSION   DAGSRM010000200 DAGSRM010000000
VERSION     DAGSRM010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.206"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            24..100
                     /locus_tag="MJE33_003637"
     tRNA            24..100
                     /locus_tag="MJE33_003637"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58..60,aa:Arg,seq:tct)
     gene            108..183
                     /locus_tag="MJE33_003638"
     tRNA            108..183
                     /locus_tag="MJE33_003638"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:141..143,aa:Gln,seq:ttg)
     gene            271..359
                     /locus_tag="MJE33_003639"
     tRNA            271..359
                     /locus_tag="MJE33_003639"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:305..307,aa:Ser,seq:tga)
     gene            363..438
                     /locus_tag="MJE33_003640"
     tRNA            363..438
                     /locus_tag="MJE33_003640"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:396..398,aa:Phe,seq:gaa)
     gene            445..518
                     /locus_tag="MJE33_003641"
     tRNA            445..518
                     /locus_tag="MJE33_003641"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:479..481,aa:Met,seq:cat)
     gene            533..609
                     /locus_tag="MJE33_003642"
     tRNA            533..609
                     /locus_tag="MJE33_003642"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:567..569,aa:Ile,seq:cat)
     gene            633..709
                     /locus_tag="MJE33_003643"
     tRNA            633..709
                     /locus_tag="MJE33_003643"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:667..669,aa:Pro,seq:tgg)
     gene            717..793
                     /locus_tag="MJE33_003644"
     tRNA            717..793
                     /locus_tag="MJE33_003644"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:751..753,aa:His,seq:gtg)
     gene            802..877
                     /locus_tag="MJE33_003645"
     tRNA            802..877
                     /locus_tag="MJE33_003645"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:835..837,aa:Lys,seq:ttt)
     gene            885..958
                     /locus_tag="MJE33_003646"
     tRNA            885..958
                     /locus_tag="MJE33_003646"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:917..919,aa:Cys,seq:gca)
BASE COUNT          233 a          212 c          254 g          263 t
ORIGIN      
        1 tttgatatac atatataaat tgtgggtgca tagctcagct ggatagagca acgcccttct
       61 aaggcgtgtg tccggggttc gaatccctgt gcgctcacca catatattgg ggattagcca
      121 agtcggtaag gcacatgact ttgactcatg tatgcgtagg ttcgagtcct gcatccccag
      181 ccaaatgatg tattactaag ctatatttga attattttat tttaaatata gttaggttca
      241 attttagaat aagtttaagt atccgaatgt ggagaggtgt ccgagtggtt taaggagctg
      301 gtcttgaaaa ccagtgactt cgaaaggggc cgtgggttcg aatcccaccc tctccgccaa
      361 atgcccagat agctcagtcg gtagagcagg ggactgaaaa tccccgtgtc ggtggttcga
      421 ttccgcctct gggcaccaaa atatggcggt atagcttagt tggctagagc gttcggttca
      481 tacccgaaag gtcataggtt cgactcctat taccgctact aaaatttaat gtgggccatt
      541 agctcagttg gttagagcgc ccggctcata accggtaggt ctggggttcg agtccctgat
      601 ggcccaccac attaaattaa ttaatataaa ttcgaggtgt agcgcagttt ggtagcgcac
      661 atggtttggg accatggggc cgggggttcg agtcccttca cctcgaccaa tataaggtgg
      721 gtatagctca gttggttaga gcgccagatt gtggctctgg aggtcgtgag ttcgactctc
      781 attatccacc ccaattcata tggtctatta gctcagtcgg tagagcacct gacttttaat
      841 cagggtgtcc cgcgttcgag tcgcggatag atcaccaaaa aggtggcgac atagccaagt
      901 ggtaaggcag tggactgcaa ctccttgatc cccagttcga atctgggtgt cgcctccaaa
      961 at
//
LOCUS       DAGSRM010000201          898 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.207, whole genome shotgun sequence.
ACCESSION   DAGSRM010000201 DAGSRM010000000
VERSION     DAGSRM010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.207"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            311..718
                     /gene="tnpA"
                     /locus_tag="MJE33_003647"
     CDS             311..718
                     /gene="tnpA"
                     /locus_tag="MJE33_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBY9649528.1"
                     /translation="MEVTHGRGYVYLVQYHIVWCVKYRHPVLTNEIEEDLINILNKIA
                     EDNKFQILECNSDKDHIHLLINCSPQHYIPDIIKALKGVSARLLMKKHGEQLKKKLWG
                     GHLWNPSYFVATVSENTEEQIRKYINNQKKQVK"
     gene            720..>898
                     /locus_tag="MJE33_003648"
     CDS             720..>898
                     /locus_tag="MJE33_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY9649529.1"
                     /translation="MAVVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIEVY
                     KNNKETFTYKQCSSD"
BASE COUNT          375 a           97 c          164 g          262 t
ORIGIN      
        1 ctccgaatag gaaaaacttc aacgactatt cctcttgagg gaagtacact acaagctatc
       61 ggtagtggaa gtgggtagac cttaacggat aatgccgaag gataagatat agtctgtgct
      121 tgcatgaaag tgtaagaagt tcataagaga actgcatagg tagtagcgaa cttatgtgaa
      181 cgacaacctc aaaaatgata aaagttaaga tttgcgtttc ttacgtatgt tatttaaaaa
      241 taaatataaa aaccttaaga atacttgttt taagtaatta ctatgttaat attatgttgg
      301 gtgatgaaaa atggaagtga ctcatggtag agggtatgtg tatttagtac aataccatat
      361 agtatggtgt gtaaaatata gacatccagt attaacaaat gaaattgaag aagatttaat
      421 taatatatta aacaaaatag ctgaagataa taaatttcaa atattagaat gtaatagtga
      481 taaagatcac atacatttac taataaattg ttcaccacaa cactatatac cagatataat
      541 aaaagcatta aaaggtgtat cagcaaggct gttaatgaaa aaacatggag aacagttgaa
      601 aaagaaatta tggggaggac atctttggaa tcctagttat ttcgttgcta ctgtatcaga
      661 aaatacagaa gaacagataa gaaaatatat taataatcag aaaaaacaag taaagtgagg
      721 tggcagtagt ggaaaaagct tataagttta gaatgtatcc aaataaaaaa caacaagagt
      781 taattaacaa aacttttgga tgttgtagat ttgtatataa taagtatctt gctaaaagaa
      841 tagaagttta taaaaacaat aaagaaactt ttacttataa acagtgtagt tctgattt
//
LOCUS       DAGSRM010000202          753 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.208, whole genome shotgun sequence.
ACCESSION   DAGSRM010000202 DAGSRM010000000
VERSION     DAGSRM010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.208"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            <1..>753
                     /locus_tag="MJE33_003649"
     rRNA            <1..>753
                     /locus_tag="MJE33_003649"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          207 a          156 c          227 g          163 t
ORIGIN      
        1 tttaactgag cgttcagtta cttgacgtag acccgaaacc gggcgaccta cccatgagca
       61 ggatgaagcg aaagtaaaat ttcgtggagg tccgaaccca cgagcgttga aaagctcggg
      121 gatgacttgt gggtagcggt gaaattccaa tcgagcccgg agatagctgg ttctccccga
      181 aatagcttta gggctagcct caaggtgaga gatacggagg tagagcactg aatgtcctag
      241 ggggtattgc acctaccgaa gactatcaaa ctccgaatgc cgtcatctta tacttgggag
      301 tcagactgtg ggtgataaga ttcatagtcg aaagggcaac agcccagatc gtcagctaag
      361 gtccctaaat gtaagttaag tggtaaagga tgtgggattg cacagacaac caggatgttg
      421 gcttagaagc agccactcat tcaaagagtg cgtaatagct cactggtcga gtgatcctgc
      481 gccgaagatt tccggggcta aaacttacta ccgaagctac ggcatcagta atgatgggta
      541 ggggagcttc ccatacgggt tgaagcatga ccgtaaggac atgtggacag tatgggagtg
      601 agaatgttgg catgagtagc gagatgtggg tgagaatccc acaggccgta aacccaaggt
      661 ttccagggga aggttcgtcc gccctgggtt agtcgggacc taagctgagg ccgaaaggcg
      721 taggtgatgg acaacaggtt gatattcctg tac
//
LOCUS       DAGSRM010000203          728 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.209, whole genome shotgun sequence.
ACCESSION   DAGSRM010000203 DAGSRM010000000
VERSION     DAGSRM010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.209"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..254)
                     /locus_tag="MJE33_003650"
     CDS             complement(<1..254)
                     /locus_tag="MJE33_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9649530.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKIS"
BASE COUNT          299 a          109 c           85 g          235 t
ORIGIN      
        1 ttagatatct taaacttaat gtgaagaatc cctgtaacaa gaccagcagt acaaccacat
       61 aagatagcca ttaaagttgc tactacagga gaaataccat tagtaagaga aaatgctaca
      121 atagaagcac ccattgtata gctaccatca gcagacatat ctggaaaatc taagattttg
      181 taagttatat aaacccccag tgccataatc gataaaatta aactttgcgt cattacagat
      241 ataatacctg acataaataa aacacccctt tgaaaatatt ttaaatggtt ttttaaattt
      301 gattttaaaa aatgattttg atataaaaaa accacacagt atatataact gtatggcaaa
      361 aattcgaata agccacacag tataatccat gtggctctat attatgatga taaaataaaa
      421 actttaacaa caattactat agccaacata gatacaatcc ttaaaaatca taatcctaag
      481 gggcttgtat ctatgttatg acaatttgtc ttaacttagg tacaaaccct aactaaattg
      541 gctactaaaa aatcgtactt gtttgtttaa taaagtcttc atatttacct ctccttttaa
      601 tttaataatt ttgaaaattt tgttttctgt tttgtatagt aattagttta atacagtaaa
      661 aaataaaagt caactataaa ttttgaaaaa ttatcaaatt atcagaaaat tgagtttaaa
      721 taaaaaaa
//
LOCUS       DAGSRM010000204          664 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.210, whole genome shotgun sequence.
ACCESSION   DAGSRM010000204 DAGSRM010000000
VERSION     DAGSRM010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.210"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..>664)
                     /locus_tag="MJE33_003651"
     rRNA            complement(<1..>664)
                     /locus_tag="MJE33_003651"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          152 a          191 c          151 g          170 t
ORIGIN      
        1 gatccagccg caccttccga tacggctacc ttgttacgac ttcaccccag ttattgattc
       61 caccttcgac gacttcttcc aaaaggttag ataatcggct tcgggcgtct ccaactcccg
      121 tggtgtgacg ggcggtgtgt acaagacccg ggaacgcatt caccgcagca ttctgatctg
      181 cgattactag taactccagc ttcatgtagg cgagtttcag cctacaatcc gaactgagag
      241 tagctttaag ggattagctc caccttacgg cttggcaacc ctctgtacta cccattgtag
      301 cacgtgtgta gccctaagca taaggggcat gatgatttga cgtcatcccc accttcctcc
      361 aggttatccc tggcagtctc tctagagtgc ccaacttaat gatggcaact aaagacaagg
      421 gttgcgctcg ttgcgggact taacccaaca tctcacgaca cgagctgacg acaaccatgc
      481 accacctgtc accaatgtcc ccgaagggaa ctctccgatt aaggagatgt cattgggatg
      541 tcaagcttag gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc cgctacttgt
      601 gcgggtcccc gtcaattcct ttgagtttca ctcttgcgag cgtacttccc aggcggagta
      661 ctta
//
LOCUS       DAGSRM010000205          646 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.211, whole genome shotgun sequence.
ACCESSION   DAGSRM010000205 DAGSRM010000000
VERSION     DAGSRM010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.211"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            56..>646
                     /locus_tag="MJE33_003652"
     CDS             56..>646
                     /locus_tag="MJE33_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBY9649531.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEARIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKG"
BASE COUNT          258 a           69 c          143 g          176 t
ORIGIN      
        1 tgaaatatca aaataaaacg tcaacaaatt aaaaattata tgggggaata tcaaaatgtt
       61 aaatgaaaca agaaaaaaac aattaactga agtatgccaa gacttaataa gaaatgctag
      121 ttattcaggt caagaagaaa acgtagtaaa agctatagaa gaaaattttg ctttactagg
      181 ttttgatagt tggtcaagag atagatatgg aaatatagta ggatgtataa aaggaaataa
      241 accaggtaaa aaaatattat ttgacggaca tatagataca gttccagttc cagatgcaag
      301 taaatggagt gttcctcctt ttgaagcgag aatagtagat gggaaaatat atggtagagg
      361 aacttctgat atgaaggggc aagtaagtgc tatgatggct gctgtttcat actttgcaga
      421 agatactaac aaagactttg aaggtgaatt gtatgtagca ggtgttgttc atgaagaaat
      481 atttgaaggt gtttcagcta gagagataag taaggctgtt caacctgatt atgttgtaat
      541 aggtgaatct tcagaattaa acctaaagat aggtcaaaga ggtagaggag aaatagtagt
      601 agaaactttt ggtaaaccag ctcactctgc taatccagaa aaaggt
//
LOCUS       DAGSRM010000206          627 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.212, whole genome shotgun sequence.
ACCESSION   DAGSRM010000206 DAGSRM010000000
VERSION     DAGSRM010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.212"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            <1..>627
                     /locus_tag="MJE33_003653"
     CDS             <1..>627
                     /locus_tag="MJE33_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBY9649532.1"
                     /translation="IKLPKLGMVKVRDKQVPQGRILNATISKEPSGRYYVSLCCTNVD
                     IEVFENTNNQIGLDLGIKEFCISSCGDFIENPKYLKKSLNKLAKLQRELSRKTIGSLN
                     RNKARLKVARLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNMIRNRKLSRLIS
                     DVSWSEFVRQLKYKANWHGRQIVKVGKFFASSQICNKCGYKNEEMKDL"
BASE COUNT          262 a           68 c          108 g          189 t
ORIGIN      
        1 atataaaact acctaaactt ggtatggtta aggtaaggga taaacaagta cctcaaggta
       61 gaatacttaa tgctactata tctaaagaac caagtggtag atattatgta tcattatgct
      121 gtactaatgt agatattgaa gtatttgaaa atactaataa tcagataggt ttagatttag
      181 gaattaaaga attttgtatc tcaagttgtg gagactttat agaaaatcct aagtatctta
      241 agaagtcatt aaacaaactt gctaaattac aaagagaatt atcgagaaag acaatcggta
      301 gtttaaatag aaataaagca agattaaaag ttgcaagact tcaagaacat atagctaatc
      361 aaagaaatga ttttctacaa aaactatcta ctaaattaat aaaagagaat gatattattt
      421 gcatagaaga tttacaagta aagaatatga ttagaaatcg taaactatct cgcttaattt
      481 ctgatgtgtc atggtcagaa tttgttcgtc aattaaaata caaggctaat tggcatggta
      541 gacaaattgt aaaggtaggt aaattctttg caagttcaca aatatgcaat aaatgtgggt
      601 ataaaaacga ggaaatgaag gacttaa
//
LOCUS       DAGSRM010000207          598 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.213, whole genome shotgun sequence.
ACCESSION   DAGSRM010000207 DAGSRM010000000
VERSION     DAGSRM010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.213"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            <1..>598
                     /locus_tag="MJE33_003654"
     CDS             <1..>598
                     /locus_tag="MJE33_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBY9649533.1"
                     /translation="LDAFITIVSSIIEIWSCKKVFDYTSQSKTDLKKLNMYFLFSVFL
                     IISSLYINIGANSRILICIFVMFVFYKSNYKVNIYKCLVISFFYWLILIGINGLSMLL
                     VATVSNLDNMNVLLSGSLYRYEAIFLGKIMLGILLYVYKASFKNFKISKKEIYICIPM
                     IANLLSFFVLYDYIINLVKISPERRTAFLVIVLLLILSN"
BASE COUNT          208 a           54 c           81 g          255 t
ORIGIN      
        1 gttagatgca tttataacaa ttgtttcttc tattatagaa atatggtcat gtaaaaaagt
       61 ttttgattat actagtcagt caaagaccga cttaaaaaaa ctaaatatgt attttctctt
      121 ttctgttttt ctcattattt cttctttata tataaatata ggagctaata gtagaatctt
      181 gatatgtata tttgtaatgt tcgttttcta taagtctaac tataaggtta atatatataa
      241 atgtctggta atctcatttt tttattggtt aatattaata ggtataaatg gattaagtat
      301 gttattagta gcaacagtaa gcaatttgga taatatgaat gttttactta gtggcagttt
      361 atatagatat gaagcaatat tcttgggaaa gataatgtta ggtatattac tttatgttta
      421 taaggcttct tttaaaaatt ttaaaataag taaaaaagag atatacatat gtattcctat
      481 gatagcaaat ttattgtcat tttttgtact ttatgattat attattaatc ttgtaaaaat
      541 ctctcctgaa agaagaactg cttttttagt aatagtttta ctattaatat tatctaac
//
LOCUS       DAGSRM010000208          587 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.214, whole genome shotgun sequence.
ACCESSION   DAGSRM010000208 DAGSRM010000000
VERSION     DAGSRM010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.214"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            <1..584
                     /locus_tag="MJE33_003655"
     CDS             <1..584
                     /locus_tag="MJE33_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBY9649534.1"
                     /translation="NREGRSIKQFRNKMYAYISKRLSKCKKGSRQSKKLWRLKNKIRS
                     KTDNQLMNLYHQTTRKFIDFCVEQKVCEIVLGDIKGVEKDTKKKKRLNRVNRQKISQM
                     EYGRIKDYIKYKAKEQGIEVKLVKENYTSQTCPKCSKKHKPTGRTYSCTCGYETHRDI
                     VGAWNILNKKHKYGLVDFRINHKQPINLKVSTV"
BASE COUNT          265 a           55 c          107 g          160 t
ORIGIN      
        1 ctaatagaga aggcagaagt attaaacaat ttagaaataa gatgtatgct tatatatcta
       61 aaagattaag taaatgtaaa aaaggtagta gacaatcaaa gaagttatgg agattaaaaa
      121 ataagataag aagtaaaaca gataatcagt taatgaattt atatcatcaa acaactagaa
      181 agtttataga cttttgtgta gagcagaagg tatgtgaaat agttcttggg gatataaaag
      241 gggttgaaaa agatactaag aaaaagaaaa gattaaacag agttaataga cagaaaatat
      301 ctcaaatgga gtatggtcga ataaaggact atataaaata taaagcaaaa gagcaaggta
      361 ttgaagttaa gttagttaaa gaaaactaca cttctcaaac ttgcccaaag tgttctaaaa
      421 aacacaaacc aactggtaga acttatagtt gtacttgtgg ttatgaaact catagagata
      481 tagttggtgc ttggaatatt ttaaataaaa agcataaata tggtttagta gattttagaa
      541 taaatcataa gcaaccaata aatttaaaag tatctaccgt ttgagcc
//
LOCUS       DAGSRM010000209          573 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.215, whole genome shotgun sequence.
ACCESSION   DAGSRM010000209 DAGSRM010000000
VERSION     DAGSRM010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.215"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            <1..>573
                     /gene="tuf"
                     /locus_tag="MJE33_003656"
     CDS             <1..>573
                     /gene="tuf"
                     /locus_tag="MJE33_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBY9649535.1"
                     /translation="IPAPERDTDKPFLMPVEDVFSITGRGTVATGRVERGVLKVQDEV
                     ELVGLTEAPRKVVVTGVEMFRKLLDQAQAGDNIGALLRGVQRNEIERGQVLAKTGSVK
                     AHTKFTAEVYVLKKEEGGRHTPFFDGYRPQFYFRTTDVTGACKLPEGIEMVMPGDNVT
                     MEVDLINSIVVEEGLRFSIREGGRTVASGV"
BASE COUNT          242 a           77 c          140 g          114 t
ORIGIN      
        1 atataccagc tccagagaga gatacagata aaccattctt aatgccagta gaggacgtat
       61 tctcaatcac aggaagagga acagttgcaa caggaagagt ggaaagagga gtactaaaag
      121 tacaagacga agtagaatta gtaggattaa cagaagcacc aagaaaagta gtagtaacag
      181 gagtagagat gttcagaaaa ttattagacc aagcacaagc aggtgataat ataggagcat
      241 tattaagagg agtacaaaga aacgagatag aaagaggaca agtactagca aagactggat
      301 cagtaaaggc acacacaaag tttacagcag aagtatatgt acttaaaaaa gaagagggtg
      361 gaagacatac accattcttt gatggatata gaccacaatt ctatttcaga acaacagacg
      421 taacaggagc ttgtaagtta ccagaaggaa tagagatggt aatgcctgga gataacgtaa
      481 caatggaagt agacttaata aactcaatag ttgtagaaga gggattaaga ttctcaataa
      541 gagaaggtgg aagaacagta gcttcaggag ttg
//
LOCUS       DAGSRM010000210          560 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.216, whole genome shotgun sequence.
ACCESSION   DAGSRM010000210 DAGSRM010000000
VERSION     DAGSRM010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.216"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            <1..>560
                     /locus_tag="MJE33_003657"
     rRNA            <1..>560
                     /locus_tag="MJE33_003657"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          151 a          115 c          184 g          110 t
ORIGIN      
        1 tgattagcta gttggtaagg taacggctta ccaaggcgac gatcagtagc cgacctgaga
       61 gggtgatcgg ccacattgga actgagacac ggtccaaact cctacgggag gcagcagtgg
      121 ggaatattgc acaatgggcg aaagcctgat gcagcaacgc cgcgtgagtg atgaaggcct
      181 tcgggtcgta aaactctgtc ctcaaggaag ataatgacgg tacttgagga ggaagccccg
      241 gctaactacg tgccagcagc cgcggtaata cgtagggggc tagcgttatc cggatttact
      301 gggcgtaaag ggtgcgtagg cggtctttca agtcaggagt gaaaggctac ggctcaaccg
      361 tagtaagctc ttgaaactgg gagacttgag tgcaggagag gagagtggaa ttcctagtgt
      421 agcggtgaaa tgcgtagata ttaggaggaa caccagttgc gaaggcggct ctctggactg
      481 taactgacgc tgaggcacga aagcgtgggg agcaaacagg attagatacc ctggtagtcc
      541 acgctgtaaa cgatgagtac
//
LOCUS       DAGSRM010000211          537 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.217, whole genome shotgun sequence.
ACCESSION   DAGSRM010000211 DAGSRM010000000
VERSION     DAGSRM010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.217"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            <1..414
                     /locus_tag="MJE33_003658"
     CDS             <1..414
                     /locus_tag="MJE33_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9649536.1"
                     /translation="LGINIGSIKILGLMISNGLIALSGSLMAQFLGFSDVNMGIGTLV
                     LGIASIIIGITLFKKFTFIKDTTAIIVGSFIYQFTIYFAMSLGMLSTDLKLITAIVII
                     AFLATGNLNISLKKNKCKASTKNKSEKRGVVDVTN"
     gene            401..>537
                     /locus_tag="MJE33_003659"
     CDS             401..>537
                     /locus_tag="MJE33_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9649537.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KS"
BASE COUNT          195 a           72 c           83 g          187 t
ORIGIN      
        1 ctaggtataa atataggttc tataaaaata ctaggactta tgatttcaaa tggacttata
       61 gctttatctg gtagtcttat ggctcagttc ttgggattct cagatgtaaa tatgggtata
      121 ggaactttag ttcttggaat tgcctctatc ataattggta ttacattatt taagaaattt
      181 acttttatca aagatactac tgcaataatt gttggctctt ttatatacca attcactata
      241 tactttgcaa tgagtttagg aatgttatca actgacttaa aactaataac tgccatagtt
      301 ataatagctt tcctagctac tggtaactta aatatttcac taaaaaaaaa caaatgcaaa
      361 gctagtacca aaaataaatc agaaaaaaga ggtgttgtag atgttacaaa ttaaaaatct
      421 ttcaaagagt ttccctaatc catatggtga acctaatact atctttgaaa atttatcaat
      481 cgatatagaa gatggtgaat ttgtaagtat cataggtagt aatggaactg gtaaatc
//
LOCUS       DAGSRM010000212          493 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.219, whole genome shotgun sequence.
ACCESSION   DAGSRM010000212 DAGSRM010000000
VERSION     DAGSRM010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.219"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            188..304
                     /gene="rrf"
                     /locus_tag="MJE33_003660"
     rRNA            188..304
                     /gene="rrf"
                     /locus_tag="MJE33_003660"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            310..398
                     /locus_tag="MJE33_003661"
     tRNA            310..398
                     /locus_tag="MJE33_003661"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:344..346,aa:Leu,seq:taa)
     gene            410..486
                     /locus_tag="MJE33_003662"
     tRNA            410..486
                     /locus_tag="MJE33_003662"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:444..446,aa:Ile,seq:cat)
BASE COUNT          156 a           91 c          109 g          137 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgctaactc tatattttta aacttgtcaa
       61 aacttatgaa tttttaatag ttttataagg caaaaaatcg aataaaaaaa gtattgacac
      121 aataagacaa agatgttatt ataatacttg tcttagaaaa aaagttaaat gaaaatttag
      181 acaataatct ggttataata gcagagagga tacacctgtt cccattccga acacagaagt
      241 taagctctct agcgctgatg gtacttggtg ggaaactgcc tgggagagta ggacatagcc
      301 aggtgatgtg ccgaagtggc ggaactggca gacgcacagg acttaaaatc ctgcgggact
      361 tacctctcgt accggttcga ttccggtctt cggcaccaca aattattgtg ggccattagc
      421 tcagttggtt agagcgcccg gctcataacc ggtaggtctg gggttcgagt ccctgatggc
      481 ccaccacaat aat
//
LOCUS       DAGSRM010000213          404 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.220, whole genome shotgun sequence.
ACCESSION   DAGSRM010000213 DAGSRM010000000
VERSION     DAGSRM010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.220"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            119..235
                     /gene="rrf"
                     /locus_tag="MJE33_003663"
     rRNA            119..235
                     /gene="rrf"
                     /locus_tag="MJE33_003663"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            242..316
                     /locus_tag="MJE33_003664"
     tRNA            242..316
                     /locus_tag="MJE33_003664"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:274..276,aa:Asn,seq:gtt)
     gene            321..395
                     /locus_tag="MJE33_003665"
     tRNA            321..395
                     /locus_tag="MJE33_003665"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:354..356,aa:Glu,seq:ttc)
BASE COUNT          128 a           76 c           96 g          104 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgttaactc taaaaaaaaa taaaaaaagt
       61 attgacaaaa atagtaaaaa atgttaatat tatacttgtc ctagcaacaa gaaaaaaatg
      121 tggttataat agcggagaag atacacctgt ttccattccg aacacagaag ttaagttctc
      181 tagcactgat ggtacttggt gggaaactgc ctgggagagt aggacatagc cacgtgaatg
      241 ttccaaggta gctcaacggt ggagcaaccg gctgttaacc ggtaggctgt gggttcgagc
      301 cccacccttg gagccattat ggctcgttgg tcaagaggtt aagacaccgc cctttcacgg
      361 cggtaacagg ggttcaattc ccctacgagt caccatttgt ggga
//
LOCUS       DAGSRM010000214          376 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.221, whole genome shotgun sequence.
ACCESSION   DAGSRM010000214 DAGSRM010000000
VERSION     DAGSRM010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.221"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..294)
                     /locus_tag="MJE33_003666"
     rRNA            complement(<1..294)
                     /locus_tag="MJE33_003666"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          103 a           88 c           60 g          125 t
ORIGIN      
        1 ccaataaaga aaactttatt ggtttgggct aatccgcgtt cgctcgccgc tacttacgga
       61 atcgaatttc tttctcttcc ttcaggtact tagatgtttc agttccctga gttcccctca
      121 ttaagctatg tattcactta atgatactta gacattactc taagtgagtt tcctcattcg
      181 gaaatcttcg gatcaaagtt tacgtgcaac tccccgaagc ttatcgcagc ttatcacgtc
      241 cttcatcggc tcctagtgcc aaggcatccg ccctgcaccc ttaataactt gaccagttaa
      301 aaaggtttga tagattagaa gctatcaact tcgataattt taaaaagtta tcagctttat
      361 atattacaaa ttagat
//
LOCUS       DAGSRM010000215          363 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.222, whole genome shotgun sequence.
ACCESSION   DAGSRM010000215 DAGSRM010000000
VERSION     DAGSRM010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.222"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            167..283
                     /gene="rrf"
                     /locus_tag="MJE33_003667"
     rRNA            167..283
                     /gene="rrf"
                     /locus_tag="MJE33_003667"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            290..361
                     /locus_tag="MJE33_003668"
     tRNA            290..361
                     /locus_tag="MJE33_003668"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Asn,seq:gtt)
BASE COUNT          116 a           61 c           78 g          108 t
ORIGIN      
        1 ttcattaaga tatatgtgca gtttttagag tgttaactct atgtaaagta ctaacttcaa
       61 ttgatgaagt atatatcgta aaaacaatta ctatttagta cttcaaaact attgacattt
      121 aaaaataaag atgttaatat aatacttgtc ttataaatag gcaatatgtg gttataatag
      181 caaagaggat acacctgttc ccattccgaa cacagaagtt aagctcttta gcgctgatgg
      241 tacttggtgg gaaactgcct gggagagtag gacgtagcca cgtgaatatt ccaaggtagc
      301 tcaatggtgg agcaaccggc tgttaaccgg taggctgtgg gttcgagccc cacccttgga
      361 gcc
//
LOCUS       DAGSRM010000216          297 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.225, whole genome shotgun sequence.
ACCESSION   DAGSRM010000216 DAGSRM010000000
VERSION     DAGSRM010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.225"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..>297)
                     /locus_tag="MJE33_003669"
     rRNA            complement(<1..>297)
                     /locus_tag="MJE33_003669"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           60 a           81 c           62 g           94 t
ORIGIN      
        1 ctacttaacg taactcgttg gcccgttcta caaaaagtac gcggtcacac aagaaatgtg
       61 cttccacagc ttgtaagtgc agggtttcag gttctatttc actcccctcc cggggttctt
      121 ttcacctttc cctcacggta ctatacgcta tcggtcacta ggtagtattt agccttggag
      181 ggtggtccct cctgcttccc acagggtttc acgtgtcccg tggtactctg gatcatatct
      241 aaagtcttct cgtttaacat acaggactat taccttctgt ggtgggtctt tccaaac
//
LOCUS       DAGSRM010000217          268 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.227, whole genome shotgun sequence.
ACCESSION   DAGSRM010000217 DAGSRM010000000
VERSION     DAGSRM010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.227"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
BASE COUNT          112 a           25 c           43 g           88 t
ORIGIN      
        1 agcagataca ggagaaatag aagtaacagt accagccaga acaacaacag tatcatcttt
       61 attgggtaga atagcagtaa atatttttaa ttttaataaa tagaattaat gtgtaaaaga
      121 gattgtgggt aagtttgcat aaaagtgaac ttacttacag tctttttggt atattagatg
      181 agtaagatta gaatactact tatataaaaa atacataaaa cttcacataa attaggttta
      241 aaaatattta tgtgtgttct ttaaataa
//
LOCUS       DAGSRM010000218          232 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.230, whole genome shotgun sequence.
ACCESSION   DAGSRM010000218 DAGSRM010000000
VERSION     DAGSRM010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.230"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..>232)
                     /locus_tag="MJE33_003670"
     CDS             complement(<1..>232)
                     /locus_tag="MJE33_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HBY9649538.1"
                     /translation="NVIQKIQQLVPPTHPVLGDGILVLTDIKSSPYPGASVVPDYCKA
                     TFDRRLLVGETREGVLAPIQALLDEMMKEDPE"
BASE COUNT           66 a           41 c           37 g           88 t
ORIGIN      
        1 aattctgggt cttctttcat catttcatct aataaagctt gtattggagc taatacgcct
       61 tctcttgttt ctccaactaa taatcttcta tcaaaagtag ctttgcaata atctggcact
      121 actgatgctc ctggatatgg agaagatttt atatctgtta aaactaatat tccatcacct
      181 aaaactggat gagttggagg tactaattgt tgtatctttt ggattacatt ag
//
LOCUS       DAGSRM010000219          206 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.denovo.231, whole genome shotgun sequence.
ACCESSION   DAGSRM010000219 DAGSRM010000000
VERSION     DAGSRM010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.denovo.231"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..167)
                     /locus_tag="MJE33_003671"
     rRNA            complement(<1..167)
                     /locus_tag="MJE33_003671"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           52 a           55 c           39 g           60 t
ORIGIN      
        1 ttatcccgta ttagcatacc tttcggtatg ttatccgtgt gtacagggta ggttacccac
       61 gcgttactca cccgtccgcc gctctttacc gaagtaaatc gctcaacttg catgtgttag
      121 gcacgccgcc agcgttcatc ctgagccagg atcaaactct caaataaaag tttatcaggc
      181 tcagatacta ttgttatcaa aatatc
//
LOCUS       DAGSRM010000220        12014 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.guided.112, whole genome shotgun sequence.
ACCESSION   DAGSRM010000220 DAGSRM010000000
VERSION     DAGSRM010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12014
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.guided.112"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            158..1564
                     /locus_tag="MJE33_003672"
     CDS             158..1564
                     /locus_tag="MJE33_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBY9649539.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLDFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            1592..2440
                     /locus_tag="MJE33_003673"
     CDS             1592..2440
                     /locus_tag="MJE33_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBY9649540.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            3101..3793
                     /locus_tag="MJE33_003674"
     CDS             3101..3793
                     /locus_tag="MJE33_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY9649541.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLAELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCAKHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            3790..4725
                     /locus_tag="MJE33_003675"
     CDS             3790..4725
                     /locus_tag="MJE33_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY9649542.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIYKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQISVESEIGKYTIFTVSLPLI"
     gene            4873..5796
                     /locus_tag="MJE33_003676"
     CDS             4873..5796
                     /locus_tag="MJE33_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9649543.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            5780..6550
                     /locus_tag="MJE33_003677"
     CDS             5780..6550
                     /locus_tag="MJE33_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9649544.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVQKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPILSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            6560..7342
                     /locus_tag="MJE33_003678"
     CDS             6560..7342
                     /locus_tag="MJE33_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9649545.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVTLVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            7718..8254
                     /locus_tag="MJE33_003679"
     CDS             7718..8254
                     /locus_tag="MJE33_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBY9649546.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            8286..8675
                     /locus_tag="MJE33_003680"
     CDS             8286..8675
                     /locus_tag="MJE33_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBY9649547.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            8712..9098
                     /locus_tag="MJE33_003681"
     CDS             8712..9098
                     /locus_tag="MJE33_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HBY9649548.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            9225..10667
                     /locus_tag="MJE33_003682"
     CDS             9225..10667
                     /locus_tag="MJE33_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBY9649549.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFART
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            10964..11779
                     /locus_tag="MJE33_003683"
     CDS             10964..11779
                     /locus_tag="MJE33_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBY9649550.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTIELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIITGCSHSGICNIVEYAKKVCRDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEIRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT         4338 a         1494 c         2259 g         3923 t
ORIGIN      
        1 aatatagagt aagtttattg taatgaattg ttaaagaaaa taattaatat agggtaaatc
       61 tatctcaatg aaataacaaa gaaaagagtt aatatagagt gaacttattg caatgaatca
      121 acaaagaaaa taatcaatat aaaaaggaga aatatttatg aatacaggat ttccaaaaga
      181 ctttttatgg ggagcatcat cttctgcatt tcaagtggaa ggagcttggg ataaagacaa
      241 taaaggaaaa accgtagccg actataattc ttttaaaaag tctcatttac aggcagatac
      301 taaagtcgca tcagactttt accataatta tgaagaagat attgaattaa tgaaagaatt
      361 agggatgaaa acatatcgtt tttcaatttc atgggcaaga ataataccag atggtgaagg
      421 agagataaac cagaaaggtt tagactttta taataagata atagacaaac taatagagtg
      481 tgatattgag ccattcgtaa ctttatacca ttttgacctt ccatttaaat tagtagagaa
      541 atataatggt tgggaaagta gagaaactgt ctatgctttt gaaagatttg ctaaaatatg
      601 ctttaagcat tttggagata gggtaaagta ttggcaacct cataatgaac agaatttaat
      661 tgttagagtg gaggagagaa ttaatatata tgatgaaaca gattcatgga agatagataa
      721 aatgcgtgct cagatggatt ataatctttg cttagcacat gcattagcag tcaatgcatg
      781 tcatgagatg ataaaagaaa gtaaaattgg agcagcagta tcatcatctg taacatatcc
      841 tcttacttct aagccagaag atgtatatgc agctagaatg aatgataatt ttaaagtgta
      901 ctatatgctt gatatgcatc attatggaga atatccaggg tattatatga agtatcttga
      961 aaaaagaaat atagttcctc acatggaaga tggggataaa gagatactta aaaaagctaa
     1021 aatggatttt atagctgtaa attattatag gacaaattgt gcagaagctt tacctgaaga
     1081 ttcacaacat ccatttggat taagagaagg aactgttgat tttagtatgt atggactatt
     1141 taaaatgtca atgaatccta acttagaggc ctctgagtat ggagcagcaa tagacccatc
     1201 aggtcttaga gttgccttaa atgaatattg gcaaagatat cacttacctg ttataataac
     1261 agaaaatggt ctaggagcta aagatatatt agaagatgga aaaattcatg atgactatcg
     1321 tatagactat ttaagaagtc atataaatgc atgtaaacta gctattgaag atggtgtaga
     1381 gatgattgga tattgtccat ggtcatttac agacctttta agttcttctc aaggttttaa
     1441 taagcgttat ggacttgttt atataaatag aactgaccat gaagttttag atttaaaaag
     1501 aatcaagaaa gatagttttt actggtataa agaagttata gagaacaatg gaatagtaaa
     1561 ataaacataa atatatattg agggttttag tatgtacatt acaaaggtat taaataattc
     1621 gttgctactt gcaaaggatg ataatgatga agaaataata ttgatgggga aaggcattgg
     1681 ccataattat aaggccgggt ctgagttaaa taaaaaagat atagaaaaga tttttgttct
     1741 tcatgatgaa aatataaaaa agagcattat acaattggca acagaaatag atgcagaata
     1801 ttttggtata gctcaaatga taattgcata tggaatagaa aaatataact taaaacttat
     1861 gaaccatata tatttggcct taactgacca cattgccttt gcagtgagac gttttaaaga
     1921 aggtataatg attgaaaatc actatttgtt cgaaatcaag gaatttaatc caaaagagta
     1981 tgatatagga aaatatgcta ttactgtttt taaggaggtt cttggtttag acctaccaga
     2041 agaagagatt ggtaatatag caggccattt tataaatgca caacaagata atccttatag
     2101 tgatagaaat aagagaagtg ctaaaattgt aaatgctatt ttgcaaatag ttcattatca
     2161 cttttctatt gtatataata aagagtcatt ttattatagg agatttgtaa tgcatctaaa
     2221 ggccttttca caaagatttc tggctaatga accatcaaga gaaaaaatag attttatata
     2281 tgagcaagtt caaaaaaatt gtaaggaaga gtatgaatgt gtgaaaaagg taggagctta
     2341 tatgtataag gaatactcca gagaattacc aagacaagag gagttatatt tgatgattca
     2401 tattcataag attttaggag aattatgcga aaatagttag aatatcttaa tatgctagta
     2461 atattataaa aaattaaaaa ttaactacac tttaaatatg aataattgca ttacttaagg
     2521 gggttgtctt aaaattgatt tttttaatta aatttgaagc tcaactcctt ttttaaacct
     2581 ttatatcatt acaactttaa tgtttatacg cctgtacttc agatgtatat ctacttttta
     2641 catttctcaa catgcaaatc taccattttt tgcagtatgt taattgaaat aaaccattga
     2701 gttaacccta aatttattct ggacttatag gaatagttcc aaagatactt taaaatattt
     2761 tctggaataa ttgcttattt cacatatgtt ttattgtgat gttttgattt aataaattct
     2821 tcaactctgt atatagtaaa tgtattatca tgtttgtttt attcaagcac acttataata
     2881 cgcttgattt tttcacaatg atgaaatata ctaatgggtg ttataagtat aagaatagca
     2941 ttacacttga agaagcaagt gataaatgag gtataaatcc tcatatgata aactactatt
     3001 tcattgagga gcatattcta aatgatgtaa agatactaca atatgactaa attcctaatg
     3061 ataaaaaagt ctacgaatga gtgtagaaag gggaactaag atgaataaag ttttaattat
     3121 agatgatgat aaagatcttt gtattttgtt aaaaaagagt atctcaattg aacagattga
     3181 agcaaactat tgccattctg ggagagatgg actggctgaa ctttcaaaac aagattatca
     3241 gttagtagtt ctagacgtga tgatgccagg tatggatggt tttgaaacta tggaacaaat
     3301 acgaaaatat agcagtattc ccatcttaat gctgacatca aaaagcgata attacagcaa
     3361 agtgcatgga ttgcgagctg gtgcagatga ctatctaacc aaaccatttg aaattacaga
     3421 atttactgct cgtgtactgt ctttaatccg acgttacacc cgttttaatc caaaaaatga
     3481 ggagccacag caactttctt tcaaaggttt gttgattgac cttgaccagc atacagtgac
     3541 taccaatggc gaacctatgt tgcttccacc caaggaattt ggtgtgcttc tttactgtgc
     3601 aaagcatcag ggtaagattt tgacaaaaca gcagatttat gaagccgtgt ggggtgagcc
     3661 atatgtatat gatgacagca atatcatggc tattatcagt cgcctaagaa aaaaaattga
     3721 actagaccca tcatctccca tctatttgca aacaataaaa ggaattgggt atcaattcaa
     3781 cacggaggta tgaaaatgga cgaacaaacg ataattcttt tcttactctt gctgtcgatt
     3841 ggtctacttt ttctttccat atttgctttt tccaagcttg ggcatattta caagcgccta
     3901 aaagatatag aggagatact ggctgatgta caaattggag acgaaaatcg taaaatacta
     3961 attaagccat gtgatgttat ggctccattg gtttatcagc ttaatgagat tgtctacgat
     4021 tatgaaaata agttactttc tctgaaaaag tcggataaag ctagcaaaca acttatgaca
     4081 agtttatctc atgatgttcg tacaccatta acaactctga ttggttatct ggatgctgta
     4141 cacagtggta tagttatcga acaagaacga gaggaatacc ttgaaatcgc taggcgtaga
     4201 gcctacgacc tcaaggatta cattgatgtt ctttttgact ggtttcggtt gaactctgat
     4261 gaatttacgc tgtcaataga atctgtagaa attgcagagt tgtcacgcaa tattttgaaa
     4321 gactggattc ctatattcca tgaaaagaaa ctgaattttg aaattgatat tccagaaaat
     4381 cgactaatgg taaacctcga cccagatgga tattcccgtg tggtcaataa tctggttcaa
     4441 aatgttcttg ctcacagtaa ggctcggcaa atcaagatta ccatgagtga ggattccaga
     4501 atggtattac tccgtgttga ggatgatggt gttgggattg ccagagaaaa tttaccacat
     4561 atttttgagc gactgtataa gtgtgataaa ggacgttcag aaaaaggaag tggtcttggt
     4621 ctatctattg tcagtcaaat ggtggagcga atgggtggtc agatttccgt agaaagtgag
     4681 attggaaagt ataccatatt tacagtgagc ttaccattaa tataaaattg tttttccccc
     4741 atgtgacatg tatacacagg gggatttttt gtaagttata aagttgcaag gttaatacaa
     4801 ggttgttgca aggttaatgt aagattgagg tggtatgata actttaataa acaaaggagg
     4861 cttttattat ttatgaataa tttaattatt gaaacaaaaa atctcactaa gcaatatggt
     4921 gctcaaaaga gcgtttctaa tttgaatatt cacgttcaga aaggtcgcat ttacggattg
     4981 ctaggaagaa acggtgcggg taagacgaca actatgaaaa tgttattggg tctaacaaaa
     5041 ccaactactg gagaaattaa aatatttggt caggacattc acaataacga gaagaagctt
     5101 cttcctcgaa ttggatgttt gattgaatca ccagggtttt atcctaatct gacaggaaca
     5161 gaaaatctca aaatctttgc aaggcttaga ggaataccaa aaaaaaatgc agtagaatca
     5221 gcactggatg tagtcggttt gccaaacaat gacaaaaagt tattttctca atactctctt
     5281 ggtatgaaac agaggttggc gattgctctt gccattatgc atgacccaca actgttgatt
     5341 ttggacgagc ctatcaatgg gcttgatcct attggtattg cagaagtaag aacatttatt
     5401 cgtgatctct gcacagagcg aggtaaaact attttgattt ctagtcacat tctttctgaa
     5461 attgccttgt tggcagatga cattggcatt attgaccatg gtattttgtt ggaggaagaa
     5521 agtcttgccc aattagaaga aaaaaacagt aaatacatac attttgctgt atctgatgca
     5581 gcacaggcag caaggcttct ggaaacccaa cttaacctga aaaatttcaa agtagcagat
     5641 aattacaata ttatgctata tgatacagat gtttctgttg cagaaataaa tcgtgctttc
     5701 attctaaaca acattgaggt atcagaatct catttatgcg aagatactct tgaagattac
     5761 ttcaagaaag taacaggggg tgaaggaatt gcttgatata atttcatgtg aattttcaaa
     5821 actcaagcgg ttaaaattta tactgatttc tattttaggt gcttgcttat ttccaattcc
     5881 cgcaacaatt ttgattgcaa aggataactt gccctttgag caattgttca aattagttgt
     5941 caattttgga tattttttgc tattaccaat tgtattaagt attgttgcta gccaactatt
     6001 ttttatagaa agagataatg atgtattaaa aaatcttgca acagtgccag tgcaaaaagg
     6061 aaaactggct ctggcaaaat tggctgtgtt gctgtttata tcattgttct actctgttgc
     6121 agggttagga gctactatca ttggaggttt cattgttgga atagtagagg gggttgctgt
     6181 aaaattagga atgagcattg cttttggaat tatgtttttt gttactgtac ttcctgttgt
     6241 ggtactcatt gtatatttca atcgcagcta tattttttct attatacttt cttttgtata
     6301 tgctattttc aattttagta tatccttaaa tattatcaat tttgaaccaa ataacccaat
     6361 attaagtgta ttacctgctc ctgttataat gcgttggtgg atggcatttt ggggagatcc
     6421 cactggcgaa tacacggcac ttagacagcc atatttgctc tcaactcctg cttgtgtagg
     6481 tattttatgt ttgataacca ttatagcagt cctgctgatt tgtatgatat acaaaaagca
     6541 ggagaattag gaggtatcta tgcttgatat aattactaca gaatttcaaa aaataaaaag
     6601 atataatatt ctatggattg gtgttgttgc tgttctgttt tcagcactat tggcagtttt
     6661 tcaggttgta agttcacacg gcagtgaccc tctaacgtat gagagctttg caaacggagt
     6721 tatctggaat aatttcagtc ttagttttcc atttgggatt acacttattg gaggatatct
     6781 tattaaccgt gaatatacag accaaacctt aaaaaacatc ctaactgttc ctatttcatt
     6841 acgcaaattg ctagtgggaa aattaattgc ggttggagga attactgtat tgttaagtct
     6901 tttcagcttc ttatgcacat ttattttggg gttgttattt tgcaatatta atatatctgt
     6961 tacactcgtt gtaaaatcct tagttcagat tttgtctgtt aatctgtact gttatgttgc
     7021 tgttttgccg attattgctt attttggtcg taaacaaaat gcatttctga cgggtgtagg
     7081 gattgcattt gtatacggtt tttgtggtgt gtttgttgct ggaagaaatc tgacagactt
     7141 ttatccaatc acagcaggtc ttggtctagt tggatacaac aatggtgctg gtactgttta
     7201 tcagccactt attggtgcta tgactttgat tgtaattcta atcttgacaa ctattctact
     7261 tgtatttaca cctaattatg ataaagttat gaccatatct aagaaaaaag ggataaagca
     7321 taaaaagtat tctcataaat aacataaata ttgtttctat tatttaagtt gctagacagt
     7381 gaaaagaaaa aatctgtcgt acagtggcta tatgctcatg tattcaagaa gctacagttt
     7441 tgatttttca taactgtagc ttctttttgt atttattaaa tcacgactat cataatactt
     7501 gcaataggta tagcgacaag agagagtact gtcatatgtg ttttatatat tacattaagt
     7561 atattctaaa atatatgttt taaataatat atttgaaaat aggaatattt aaataaaaaa
     7621 aataatatta tatatagttg acaaaaattg gcaaatgata taatatataa atatagaaat
     7681 agtaatcatt actattatta aaagggagga attaattatg aacttaaaag gaactaaaac
     7741 agaaaagaat ttaatggcag gatttgcagg agagtcagaa gcaagaaata aatatacata
     7801 ctacgcttca aaagcaagaa aagaaggtta taatcaaata gctgctatat ttgaagaaac
     7861 tgctaacaat gaaaaggaac atgctaaatt atggtttaaa cttttacatg atggtatgcc
     7921 ttcaacagag gaaaatttaa aggatgcagc agcaggtgaa aactatgaat ggactgatat
     7981 gtatgcaaaa tttgctaaag aagctagaga agaaggattt gacaagatag cttatttatt
     8041 tgaagctgtt ggaaaaatag agaaagagca tgaagaaaga tacttaaagc ttttagaaaa
     8101 cttaaatgaa ggaaaaatat ttaaaagaga tgaagaagtt gtttggcaat gtcaaaactg
     8161 tggtcatgtg tatgtaggta ctgaagcacc agaaaaatgc ccagtatgtg accatccaaa
     8221 agcatatttt aatataaaag ctgaaaatta ttaaaaatta tcattattaa aaaggaatgt
     8281 taactatgaa attttctaaa caacgagaac tgattttaaa tgaaatatta aataatccgg
     8341 ttcatcctac tgcggattac ctatatgaaa acttaaaaaa agataatcca aatttaagct
     8401 taggaactgt gtatagaaat ttagctcagc taacggaaca tggctttata agaaaggtta
     8461 gtattccagg atatccagat agatttgatg gtcgaataga taatcactat catatcatat
     8521 gtgaagtatg tggagaggta tacgatttag aatccgaagt tcttaataat ttacaagaat
     8581 taatatctga ggagacagac ataaaaataa catcttataa cataagtttt aaagggattt
     8641 gtaataattg taaaaggtgt agccaagtag gttagttttc aaattctaaa aataattaat
     8701 gaggtgagtt tatgtgtagt gaacaaaaat tttttatatg tgagacttgt ggaaaccttg
     8761 taggaatgat acaaagtggt ggtgtaccta ttttctgttg tggaaaacct atgaaggaac
     8821 ttgttcctaa tacaacagat gcagcagttg aaaaacatgt acctgtaatt gaagtggatg
     8881 gaaataatgt tactgttaaa gtaagtagca caactcatcc aatgactaaa gaacatcata
     8941 tagcatgggt gtatcttatg actgaacaag gtggtcaacg caaatgtcta gcagtagatg
     9001 gagaacctgt agttaaattt gctttgaatg atgatgataa agtgatttct gcctatgctt
     9061 actgtaattt acatggatta tggaaggctg aactgtaata tttaaataaa ttgagtcaaa
     9121 aaggaggcta taaatgtatt ttcatacaaa tagcctccta taaatttata taaattcaag
     9181 ttttaatttt gtttaatgtc aatataatat aaggagaaaa taatatgtca atttataaat
     9241 gtagtgtttg tggatatatt tatgatgaat ctaagaatga taaaacatgg gatgaattaa
     9301 gtgaagattg ggaatgccct gtatgtacaa aaggtcgtag ttattttgga aagataagta
     9361 ctgtttacta tgaagaagat gaaaagatag cagaagacat agttgaagat gaatctaaat
     9421 taaatactga aaaagaggga gatttaaatt acttaagtac atatttaaga agagatgatg
     9481 aagttgagaa acatatggat ataattcacg aaatggctgt tacaggaaaa tctataatag
     9541 aacctatgag aactaaacta ccagtaattt cttgggatga tattttaatt atgggtgctc
     9601 aattaaatcc attaccttta aatgagcatg atgaagtaaa tacaacaaca ataataggaa
     9661 aaaaagcaaa gaaacctatg attatagaaa atccagttta tatttcccac atgtcatttg
     9721 gagctttgtc taaagaacta aaaatagctt tagcaaaggg tgcagcccaa aataagacag
     9781 ctatgtgtag tggtgaagga ggtatattac cagaagagaa ggaagcttca tacaagtata
     9841 tttttgagta tgtaccaaat aaatatagtg taactgaaga aaatcttaaa aattctgatg
     9901 ctatagagat aaaaattggt caaggaacaa aacctggtat gggaggtcat cttcctggcg
     9961 aaaaagtaac tgaagaaata gcaaaagtaa gaaataagcc tgtaggtcaa gatgtaataa
    10021 gtccatcttg ttttgaggaa atacaatcca aagaggattt aaagaaactt gtagatgaac
    10081 ttagagaagt ttcagaaggg cgtccaatag gtgttaaaat ttcagcaggt catattgaaa
    10141 aagatatgga atttatagct tatgctaaac ctgattttgt aactattgat ggtcgtggtg
    10201 gagctactgg agcaagtcca aagttgctta aagatgcaac ttcaattcca acaatatttg
    10261 ctctttatcg tgctagaaaa tacatagata cacatggatt agatatagat ttggttataa
    10321 caggtgggct tcgtatatct actgattttg ctaaggcaat tgctatgggg gctgatgcag
    10381 ttgctattgc aagttctgct cttatggcag cggcgtgtca acaatatcgt atatgtggtt
    10441 ctggtaagtg tcctgtagga gtagctactc aggatgagga attgagaaaa agattacata
    10501 tagaaaacag tgctaataga gtggcaaact ttttaaatgt aagtttagaa gaacttaaga
    10561 cttttgctag aacttctggt cataaagata tacatgattt aagtgttgat gatttatata
    10621 ctgttaactc tgaaatatca aattatacaa acatacagca tgtatagatt gaagtttgaa
    10681 actattagat tttagatagc tataaaagta aatgataaat atattttata aaagagagtg
    10741 tcttaaaatg aactttttag ttcataagtc actctttttt ttatgaagcc aaatatattt
    10801 atcattttag caatgaaaaa gaatcttatc tctttatttt gagatagatt ccttctattt
    10861 atgaaaatta aagatattaa attgtaaaat attctgtgat gtcgtactat taaactaaag
    10921 attctgtact ttaaagtaaa taaaaattta aggagtgatt attatggaaa gattgaagat
    10981 acaagtttta gtcgacaata atacttatat tgatagatac tttgttggag aaccagcagt
    11041 aagctattat attgagattg atggaaatag gattttgttt gactcagggt attcagatgt
    11101 attcatatca aacgctgaaa aattaaatat agatttagga aatttaactc atgttgtttt
    11161 ttctcatggg cataatgacc atacaagagg tatccagttt ttagaaaata ggtatgattt
    11221 atctacaatt gaacttattt cacatcctaa ctgttttata cctagaaaac atggagagaa
    11281 aagcatagga gctccatttt ctgctgagga aattaaaaat atttttatgt ataatcctaa
    11341 ggataagcca tttaatctaa gcaagaattg tgtgttttta ggtgaaatac cttctataaa
    11401 tgattttgaa aaaagagcta aaattggtaa atgtaaaata ggagatttgt gggaagatga
    11461 ttatgtttta gatgattctg ctatagtttg taaaactgat aaaggtattt ttattattac
    11521 aggatgttct catagtggaa tttgtaatat agtagagtat gctaaaaagg tatgtagaga
    11581 tgatagagtt attggtatac taggtggttt tcatcttttt gaattgaata ataggttaga
    11641 tagtacaata caatatttgg ataaagaaga aataagaatg ttgtaccctt gtcactgtgt
    11701 ttcttttaag gtaaaagcga agatgaatga aatattgaat ataaatgaag ttggagtagg
    11761 gctgaccata agtatatagt atttgaaagt atataagatt taagttgaag gttaaaaaga
    11821 tattttctat agaaaatatc ttttttgtta cgtttatttt aagagaaatc aatatacttt
    11881 tatatgaaat attgtaaaaa aatttatttt taatgagaat atattttcat taataaacaa
    11941 ttttattatg tattaataat gttacaataa atataaataa atcaaagtta cttacattac
    12001 agtatatttt ttca
//
LOCUS       DAGSRM010000221        11411 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.guided.117, whole genome shotgun sequence.
ACCESSION   DAGSRM010000221 DAGSRM010000000
VERSION     DAGSRM010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11411
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.guided.117"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            278..1231
                     /gene="blaCDD"
                     /locus_tag="MJE33_003684"
     CDS             278..1231
                     /gene="blaCDD"
                     /locus_tag="MJE33_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HBY9649551.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYLGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNDKNKKEKINMVDYSDCFEDISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLINKVDAKYVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            1575..2258
                     /locus_tag="MJE33_003685"
     CDS             1575..2258
                     /locus_tag="MJE33_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9649552.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEIASLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            2255..4915
                     /locus_tag="MJE33_003686"
     CDS             2255..4915
                     /locus_tag="MJE33_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9649553.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIKLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYVNSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKNIASSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQDASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(5096..6277)
                     /locus_tag="MJE33_003687"
     CDS             complement(5096..6277)
                     /locus_tag="MJE33_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY9649554.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVVYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTIHAENCHNGGARFIIKFY
                     T"
     gene            complement(6277..6939)
                     /locus_tag="MJE33_003688"
     CDS             complement(6277..6939)
                     /locus_tag="MJE33_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY9649555.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCENISLNKVEVKAYLDNVEVPVTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DN"
     gene            complement(7071..7709)
                     /locus_tag="MJE33_003689"
     CDS             complement(7071..7709)
                     /locus_tag="MJE33_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBY9649556.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQGMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVTGKSYLNNFNIDEEKFIETTLALLIDSIKA"
     gene            7888..8493
                     /locus_tag="MJE33_003690"
     CDS             7888..8493
                     /locus_tag="MJE33_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBY9649557.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGENIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEDVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMMKSISELDNLKYEWICMSHGTPCIK"
     gene            8997..9746
                     /locus_tag="MJE33_003691"
     CDS             8997..9746
                     /locus_tag="MJE33_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBY9649558.1"
                     /translation="MRLESLVNQNYDNLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KSDLFIIISLSGETKAGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            9743..10507
                     /locus_tag="MJE33_003692"
     CDS             9743..10507
                     /locus_tag="MJE33_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBY9649559.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVIDDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVAKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDASKIIDFAKEMRMKGIYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            10606..>11411
                     /locus_tag="MJE33_003693"
     CDS             10606..>11411
                     /locus_tag="MJE33_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBY9649560.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKKREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDIHKIC
                     LWSNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDIVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELK"
BASE COUNT         4332 a         1347 c         1804 g         3928 t
ORIGIN      
        1 attgtaatat ttaaatatta caatagtaag ttttaaatgt ggaatcaatt attatatcac
       61 aaattaaaag gaggacagga tgttattaat agttacctaa ttatcttcgc aataggttta
      121 atcaacataa ttgtactagg tgaagaaaat ataagagata cagctattca tttagtcttg
      181 tttcttataa gtagaaccta gattggagta tattacaaat aatgtatact attttaatag
      241 taggttataa atttaaatat gggagatgat ataaattatg aaaagaaaaa agaattttat
      301 ttggatagct atcttattgg ttggtgtagt tatggtaatg tattatttag ggaaaaaaca
      361 taacgatata aatcaaaaaa ctgataagaa ttatataaaa tctgaattaa ataagtctga
      421 attaaataaa tccaaaattg aaaacaatga taaaaacaaa aaagaaaaaa ttaatatggt
      481 tgactatagt gattgttttg aagatattag tggaggagct attttttata atactaaaaa
      541 taaagaatat aatatatata acaaagaatt aattgaaaca agaaggtcac catgttctac
      601 ttttaaaata gtttcaactt taattggttt agaaaaaggg gtaataaatt caaaagaatc
      661 agttatggga tatgatggta cagagtatcc aaataaaaat tggaataaaa atcttagttt
      721 agaagaggca tttaaggagt cttgtgtttg gtactacaaa aaattgataa ataaagttga
      781 tgctaaatat gtccaaaaca ttttagatga tttaaagtat gggaattgcg atattagcga
      841 atgggaagga gatttaaaaa atggaaaggg acatttaaat ggattttggt tagaatcttc
      901 attacaaata tctccaaaag aacaagtgca aacaatggca aaaatatttg agggagatac
      961 aaactttaaa aaagagcata ttaatatttt aagagatatt atgaaaatag atgtaaatga
     1021 taaaaacata aatgtatatg gaaaaactgg aacaggattt gatgaaaaaa ataaacgtgt
     1081 ggatgcatgg tttgttggta tgctagagcg tgaaggtgac acctattatt ttgctattaa
     1141 atcagatgat tccaataaag aaattacagg gccaaaggta aaggaaattg caattaatat
     1201 aattaagaaa tattatagtg taagggaata aacttaattt tatttgtaaa tattatttac
     1261 ctatagtctg taatatttat taaataattt tactaaattg agtctatata gagcaaaact
     1321 cctttagtac ataaaatact tgctattgat tattaaattg aatacctata gatttaaagt
     1381 taataccttt taaagactat gtaatatatt aaaagtataa ataaaatata ttgcatagtc
     1441 tttgttttat attataattt taaataatgt gcaaaaacta aacaatattt ttaatagtaa
     1501 tttatatttg tattcatgtc actataatgt cattttaggt tgctaaaatt taaccataaa
     1561 ggagatgatt gtatatggaa atattaaaat gtgaaaatct cacaaaaata tatggttcaa
     1621 atcaaaccag agtgacagcc cttaataatg taaatttatc agttcaaaaa ggtgattttg
     1681 tatccattgt aggagcatct ggttctggca aatctacatt gttacatctg cttgggggag
     1741 tagatagacc tacatctgga aagatttatg ttgaagatac agaaatagca agtttaaagg
     1801 aagaagctct tgcagttttt agacgtagaa aagtaggtct tatttatcaa ttttataatc
     1861 ttatacctac tcttgatgtt agaaaaaata tattattacc catgttactt gataaaagaa
     1921 aggtggatga agatagattc tctgagattg tatctatatt gggattatct gatagattaa
     1981 accatcttcc aagtcaactt tcaggtggac aacaacaaag ggtatctata gctagaagtt
     2041 taatttatag accagctatt ttattagcag atgagccaac tggtaatctc gatagaaaaa
     2101 attcagaaga aattgtagat ttgttaaact tatcaaataa acgatttaat cagacaatac
     2161 ttcttattac tcatgatgag aaaatagcac tagaggcaaa tcgtattgta actatggaag
     2221 atggagtgat tgtctctgaa aaggtggtga agaaatgaat attcttcaag aatacacctt
     2281 agactttgta cgacgcaata aacgtagtag tatagctata atgttagcta tattacttac
     2341 tacaaccatg atgtcttctt tgtgtggact gttgtatacc atgtggacag attttataaa
     2401 actttcaatt gagaaagaag gtaactggca tggtgaattg tttgactata cttatggaag
     2461 tgacttacca ttaattgaaa atttttcttc agttgatgat gtaatggtaa aaggacaatg
     2521 gtatgtaggc aaaattaatg atgagaaaag agattatata atttatagaa atgctacatc
     2581 agagtattgg aattctatgc cagaaaagga tactataata gagggtcgtc gaccaaataa
     2641 tgctaatgaa attgcattat caaaacaata ttttgagaat aatccaagtg taaaaattgg
     2701 ggataaaatt actctcccaa taggagatag aatcctaaat ggaaagcctc ttgaacccat
     2761 agccccaaaa tcgaaagggg aaagttttaa aaaaatttca gaggctacat tgacaatagt
     2821 aggaaaggta gatgtttcta cttcatcagt aatacctgca tatacagctc ttggatatct
     2881 tgataaaaca agtataaaac ctaatgatag tataactgtg tacttgcgat ttaaaaatat
     2941 aagagacacg tataaggaac ttccaaaact tgctaaatca ttgggatgga agactaatga
     3001 atatggaaaa tacaatctta aatacaatag taattatctt ttaaagatgt tagtcttttc
     3061 accagaacag aaagcatcta tgagtagtat atcaaaattt tcaactccta ttatgtatct
     3121 tacaatagca atttttacag tagccgtgtt tgtaatggtg atttacaacg cgttttcact
     3181 gtctgcaaat gctagactta ctcagttagg gatattatct agtgttggtg catcaccaaa
     3241 acaaattaaa aggtctgtag tgtttgaagg ctttttactg acaattatac cattaccaat
     3301 aggtttattt ttaggatggt tattgtgtaa taggcttata gtttatgtta attcagttaa
     3361 ttatcatact gatgttccag aagtggtatt tacttatggt attcctgctt ttctgccagc
     3421 agttttactt acaatagtaa cagtctggat ttctgcactt ataccagcac gaaaagtatc
     3481 aaagatatct cctatagaag caatacgaca aggagatagt gttaaaatca aaaagtcgca
     3541 taaatattca ttaggaaaga tatttggaat agaaggagaa cttgcatcaa acgcactacg
     3601 tgctcgtaaa aaatcttatc gtactgcaac aatatcttta acattatctt ttttattact
     3661 tacatgtttt ttgcatataa ttgccaatca agaaggagca aaagctgttt atggaagtga
     3721 taattataag gaccaaagag atatagcagt ttatttggaa aatagtgaac ctgtagagtc
     3781 tgctttcatt gaaaaattag ataatacaga aggtgtaaaa agttcactat attatcaaaa
     3841 aatgtctgct tccacttggt tgactgaaaa agatgcttca aaagaacttg cgaaaaatgg
     3901 aggatttaaa aatatagctt catctaaaaa gtactctcct atagaacgtg atggaaagtt
     3961 tcgtataatt tcaaatataa ttgggcttga tgataagagt tttacagaat attgtaggca
     4021 aatttctgta gacccaaagt tattttatga tacaaaaaat ccaacctcaa ttgtatataa
     4081 taaggaggaa gatattaatc gtagtacacg tagaaataaa gtatatatag attatttaaa
     4141 tctttcaata ggagataaca ttaaattaaa tgaaaaagta tatgacgaag ataaagggga
     4201 ttatacttac aacatgaaag tgggagctat tacaagcaca cttcctaaaa tagtagatac
     4261 tagttcaaag tatacattaa tgcaaatagt tccaatgtct atggtgcagg aaatatctaa
     4321 aaattatgat gaagtaagta ggttacgttc aaataggctg ataggagtat ttaaagtaaa
     4381 taatcgtgag gacattccta gagtaagaga aaaattagag tccatttata ataaagacta
     4441 tggttcagga gattatagta ttgaggatgt tttagaaagt gaaaaacaag atgctagtgg
     4501 aataaagacc atgaatttaa tagtaggctt tattacaggt ttattggcta tgattggatt
     4561 atcaaatgta cttgctacag tgtcaggaaa tattagaagt agacgacaag agtttgctat
     4621 gttgcgttct gttggattat ctccagaagg cataaaaaag atgcttattt tggaaggttt
     4681 attcttagga attacaccat tactacttag tattcctgtg caaataggaa tagtgtatgc
     4741 ttttctacgt ataaatgaaa tatattttat agagtattta ccatttgcac ctatatctac
     4801 aataataggt tttactatat tgatactttt tattgtaata gcatcatata tgacaggata
     4861 caagcagttg aagaatgaaa atatagtaga atctataaaa aatgaaacaa tataaacaaa
     4921 atctatttga acttatttaa aggtttcgat ttaagactac ttacaagtat agggatagtt
     4981 aagttgtttg tttaataaaa ccttaaatgt aaatagaagt atattactaa aacaatagat
     5041 aacatagaat atagatatag ctggattata ggtattttac ctataatcca gcattttatg
     5101 tgtaaaattt aattatgaat cttgcaccac cattatggca attttctgca tgtatagtac
     5161 cattttgacg ttctataata gactttgata gagcaagccc tataccaata ctgtccttac
     5221 tagcattttt accacgataa aatctattaa aaatatatgg aagttcttcc tgagcaaacc
     5281 ccttaccatt atcttctatg ataatctcaa catatagagg attttgacta taataagcaa
     5341 ctatatatcc accatcttct atatgctcac tacaattttt aagaatattt aaaagagctt
     5401 ctaaagtcca attaatatcg atttcaaatt ctaccgaagg agaagcctcg atagtaaatg
     5461 tctgatgttt ttctcttaaa gaaccttcta taggctcaat tgcataagta agaagagaat
     5521 gtacatctat aagtttgctt tcaaatatta aagtacttgc atccaatttt gataatgtaa
     5581 gtaaagatga aactagtctc tcaagtcttg agatttgatt tgtaagtctg ttaatataat
     5641 ctatttcttc atctggacaa ttttctgaaa gtaattgtgc cataagtgac atagaagtaa
     5701 ttggagtctt aagttgatga gcaatatctg caagattatc tgaaagagtt tgtctttctt
     5761 ttaaagcaaa ttcttttgaa caacgaagtt ccccaactgt tttatatatc tcatcttcaa
     5821 ggtgagaaaa ttcatcttca catcgtaaaa gtacagactc ttttcctaaa ttcacttctt
     5881 ctaagtattg agtaagattg tttattctac tcagtttttg cttatttgtt ctatgtttga
     5941 ttatatatac aactgtagcc attgttgaaa ttataccaaa cgatatcact agaaaataaa
     6001 aaaagttttt actccaaaat gtacttttag aatatccatg ttttgataaa atttcatttc
     6061 ctgttttaaa atccttatca ctagaatttt tcagtgattt tagtatactt tctgtagact
     6121 taggattgta ctcatatgat gcaccaagag ccttagacag tacattgtat tcattaaaag
     6181 aagttatagt tgttagaatt acactcaata aaaaagttac aattaagcag aatattataa
     6241 aaacaactgt actgttgata tttcttggag ttttcactag ttgtcctcca ttctatatcc
     6301 tatgcctctt aatgttttta tacaaggtgg attttttaat ttttctctaa gacgttttat
     6361 tgtaacagta agagtgttat catttacgaa attaccatct acatcccaga gcatttctag
     6421 taactttgta cgagtcatag ctctattttt attttccata agacttagaa gaagtttata
     6481 ttctcctgct gtcacaggta cttcaacatt atctaaatat gctttgactt caaccttatt
     6541 caagcttata ttttcacatg taatagtatt gttagtattt gaatgagttc ttctaagaac
     6601 tgcattaatt ctggattgaa gtactgagag caaaaacggt ttaaccacat agtcatctgc
     6661 acccatatct aatccacgta taatgtcttt ttcatcatca cggactgtca aaaatatcac
     6721 tggagtatct tctatacttt taacatattg gcaaaactcg tatccaatac catcaggtag
     6781 atttaaatca agcagaatta aatcaatagt tttatcaaaa ttttttttag cttcagcaat
     6841 cgtattataa caacatacag tgtgattttt cttttcaagg gaagttttta ttccaaatgt
     6901 aattgtatca tcatcttcta aaagaagtat tttagccatt tacttttaat cctttcagta
     6961 atttagttat tccattaact aagttattag ttttatgttt aatatttttt atattagaga
     7021 tagaaaaaat tatcatttaa gtatataaac tttaatttat aaacttaggt ttatgcttta
     7081 attgaatcta ttaaaagagc aagagttgtt tcaataaatt tttcctcatc tatattaaag
     7141 ttgttcaggt atgattttcc tgttacagat agtaaattaa aaaatcctgt aagtgtaaat
     7201 attgatgaat acataagagt aggtatttca atatcatgcc taatacttct atcatttttt
     7261 ccaatttcaa acattccttg tatttcatca aacataaatt tattaaaaga aaaaaacttt
     7321 tctttaaaca gcatatcagt atccgatttt tcagagttta caataccaat cattcccata
     7381 aggcataata gagaaggttc tacagagtaa aatttatgaa aagcaaagta tgaaagtttt
     7441 attttttcaa accctgtttt gccttttgca ctttctattt taacattatc ccaaagtctt
     7501 ttatatcctc ttaagacaac agcaaagaat aaatcttctt tacatgaaaa gtacttgtat
     7561 atggtgcgtt tagtatattc aacttctttt gctaattcat tcatagttgt attatcaaat
     7621 ccattaagac aaaataattt ttctgcctta tcaataatct cattttctct ttgtattttt
     7681 tctttatctc tttttgataa ttctgacata taaattattc ctttctattg ataaaaaagt
     7741 atactgatgg tatacttatg taaatataat acttgtatac ctatagtata ctttaataaa
     7801 aaataaaatt aatatcaagt attataacta tttataactt tattaattaa ttatataggt
     7861 aaaataccaa ataaggggaa tttatttatg aagataaaag aaaatgtatt aatgttagat
     7921 agtactcgtg gctcaaattg ttttattgtg tttgataaag aaattattct tatagacaca
     7981 ggtcttcctt ttatggggga aaatataatt aaagaattaa aaacacttgg cattaaactt
     8041 acagatatta agcatatttt attaactcat catgatgttg accatatatc taatattaaa
     8101 ttattgaaag aaaaaacagg agcaaaagta tgggctcatg tagaggatat accttttatt
     8161 ataggagaga aggatagacc tgggtttaag aagtttattg gaaaggcaat ttccagaagg
     8221 ctcattaagg atgttgaacc ttatggagag aatatgcaga ttgggaatat aaaaattatc
     8281 catactcctg gtcatactcc agggcatgtc tgcatgattt ttgaagatgt attatttgca
     8341 ggtgatttag tcaaaaataa gaatggaaat atagttcctt atcctaatcc atggaattgg
     8401 gattataaaa agatgatgaa atctataagc gaattggata atttaaaata tgaatggata
     8461 tgtatgtctc atggaactcc ttgtattaaa taataaaaat taacatggat taatagaggc
     8521 atagttgctt gtatgtatga taataattta cagcttcttt ttaatatatc atgtatattt
     8581 gtagctgtaa gcatatttat aacaatactt acaattttgt attttaacat ctataataat
     8641 agttttagct ttgttaacta tgaaatttac ttaaagaagt aaatattcaa gtaatagtaa
     8701 ttattaaata agataaaaag taaatggcag atgaattaaa gtaatttcta ccatggaaaa
     8761 cacttttgag ttagaaaaaa ctctatttta gagtatgaag agatgaagct tctgaagaaa
     8821 gcaatatcta aagtacatgt tcgaaaggat aagtactttt tggtgcctgc ttttagcagg
     8881 ttatttttat tttaataaaa ataaaatcaa atgattattt tatccttatt atttaaaact
     8941 cttccctaga aaaagcaact aatgtatatt aaaataatat ttagggagtg gaaaaaatga
     9001 gattagaatc gttagttaac caaaattatg ataatttaaa tgaaaatgat aaaattatta
     9061 ttaaacaaat attgatttgt caaagagaat accgtaattt ttcgtgtgaa aaaattgcaa
     9121 atgaatgtaa tgtttctaga gctactttat taaggctttg tagaaaaatt ggattagatt
     9181 cattctctga tttaaagtat ttacttaaaa ataatcaact agaatttgtt aataatgata
     9241 ataattttca tgatgtttgc gagaattatc gactattaat gaatgaatta caaaaaatat
     9301 catttcaaga tatttgtaaa ataatatatg atgcagaaac aatttatatt tatgggacag
     9361 gtaatgaaca gaaaagttta gctcaagaaa taaagagaat atttctttca gtaggtaaat
     9421 gtgttattga cttatttgat ataggtgaaa ttgaatttat gagagaatca tttcaaaaaa
     9481 gtgatttatt tattatcatt tctttgtctg gagaaacaaa agcaggaatt gaaattgcaa
     9541 aatttatcaa caatcatata catacgattt ctttaactag gcttgataat aatacaattg
     9601 caacactttg tgagtataat ctgtatgttg aaacgcaaaa acttgataca ttacaagcta
     9661 tatcttatga agtcgtagga gcattttatg ctctattaga tttgcttcat atgaattaca
     9721 tagagtatag aaggagaaaa gaatgaaaat tgaaggactt ttcaataaat attataattc
     9781 atttacagca aatgataagt atatctgtga gtgtattctt aatcataaag aagattgtat
     9841 taaactttct atagatgaat ttgcagacaa ataccatata tctacaagtg cactttctcg
     9901 ttttgctcaa aaactacaat tacctggata tagtgaatta agagttatta tacgcttaaa
     9961 tgaaacagaa gtagaatata aaaatcaaag taaacaccaa ttaatggatt gttataataa
    10021 ggtgatagat gatatagaga agaaggattg ttcgattatg tttgaaagaa tgaaaaaggc
    10081 aaatcgtata atcgtctttg gagaaggtta ttctcaggaa agagttgcta aagagatgaa
    10141 aaggattttc ttaccaacag ggaaaattat atatgatgtt tatggttatg atatgattca
    10201 ggcacttaat cattttataa aaacagatga tatgattttc tttatttcat tgaatggaga
    10261 tgcatctaaa attattgatt ttgctaaaga aatgagaatg aaaggaattt atatggtttc
    10321 tattacaaaa atgataagta atcctttagc aagattatgt gatgaaaacc tgtatattca
    10381 atctatatct atttctgtag ataaacaact ggactacgat gtaacaacac cttattttat
    10441 tttaattgaa ctgctttata ttaaatacaa gatgtattta gaaagtgaac atattccccg
    10501 aaaatgaaaa tttgtgctaa aaatgaacat attttttaaa agatatcaaa atattgatat
    10561 cttttttttt gaatgctatt atgattttga tagtaagaaa ggaagatgaa attgtacaaa
    10621 ttattaatac ttgatataga tggtacgctt agagatgagg tatatggaat cccagaatct
    10681 gcaaagcatg ctatacgttt atgtcaaaag aatcattgca gtgttgttat ttgtactgga
    10741 agaagtatgg gtacaataca agatgatgtt ttatcattag gagttgatgg atatattgca
    10801 ggaggaggta attacattca gtatcatggt gaattacttt ataatcaatc ttttaatcaa
    10861 agattgataa aggaagttgt ttgtttatta aaaaagagag aagttgcttt ttcgattgag
    10921 agtcaggaaa aagttttcat gaatcaaaaa gccaaagaaa tatttgaaac aatgaatcag
    10981 ttgaaaggaa ctaattcttg tataaataaa caacatatcc aagaaaaaat aacatatgaa
    11041 aataacattg aagagtataa gtcacaagat atacataaaa tttgtttatg gtcaaatgaa
    11101 aaggtatttg acgaagtgaa agatatacta caagataaaa tggaattagc acagagagat
    11161 atttctagtc agtattatga aattattcaa aaagattttc ataagggcaa agcaattaag
    11221 agattacaag aaagattagg agtaactcaa aaagaaacca tatgttttgg tgatggtcaa
    11281 aatgacattg tcatgtttca agcatcagac gttacaattg caatgaaaaa tagtcatcaa
    11341 caacttaaag atatagcgac atctatatgt gaggacatat tcgataatgg aatttataaa
    11401 gaattaaaaa g
//
LOCUS       DAGSRM010000222        10932 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.guided.118, whole genome shotgun sequence.
ACCESSION   DAGSRM010000222 DAGSRM010000000
VERSION     DAGSRM010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10932
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.guided.118"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            4..621
                     /locus_tag="MJE33_003694"
     CDS             4..621
                     /locus_tag="MJE33_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBY9649561.1"
                     /translation="MCKKTYLLVVSILIAGLLTACSGSPTTDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIMTPQQAMEIALKEQNG
                     IVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            729..3260
                     /locus_tag="MJE33_003695"
     CDS             729..3260
                     /locus_tag="MJE33_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBY9649562.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            3694..4395
                     /locus_tag="MJE33_003696"
     CDS             3694..4395
                     /locus_tag="MJE33_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBY9649563.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            4445..5527
                     /locus_tag="MJE33_003697"
     CDS             4445..5527
                     /locus_tag="MJE33_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBY9649564.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFMNHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            5788..6888
                     /gene="vanG"
                     /locus_tag="MJE33_003698"
     CDS             5788..6888
                     /gene="vanG"
                     /locus_tag="MJE33_003698"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HBY9649565.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            6885..7691
                     /locus_tag="MJE33_003699"
     CDS             6885..7691
                     /locus_tag="MJE33_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBY9649566.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            7709..9847
                     /gene="vanT"
                     /locus_tag="MJE33_003700"
     CDS             7709..9847
                     /gene="vanT"
                     /locus_tag="MJE33_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HBY9649567.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKHLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVNMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINDCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLAE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            10161..10781
                     /locus_tag="MJE33_003701"
     CDS             10161..10781
                     /locus_tag="MJE33_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBY9649568.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
BASE COUNT         4141 a         1152 c         1950 g         3689 t
ORIGIN      
        1 tttatgtgta aaaaaacgta tttattagtg gtaagtatac ttatagcagg tttattaact
       61 gcatgtagtg ggagtccaac tacggattca ggacaagcac aagataatac aaaaaaggag
      121 gcaaatgcat caaataacgc tttaaaagat gaaaaaaata atgaaaattt aatggaacaa
      181 gattttaaag tgccatacac agatgcaata aacatattta aagataaata taaggatgct
      241 gatatagtag atttaagtct tgagagagat ttaaataaat ttgtttatac tgtggaaggt
      301 gtagatgata ataatgaata taagatgaaa attgatgcta atacaaaaga tgtattagaa
      361 gataaaacag aaaagttgga ttcagaagat ttaaatggag ttgctagaaa agaaaaatta
      421 gatttaaatg acataatgac acctcaacaa gctatggaaa ttgcattaaa agaacaaaat
      481 ggaatagtta aagaatggag tttagacaaa gatttagatg ttacttttta taaaataaga
      541 atagataaag ataaaaatga atatgacata aaagtagatt ctaaaaaggg aactgtattg
      601 aaggtagaaa aagaagatta gaagatttaa actatcttta aaataatttc ttttttgaat
      661 aaattttgtt taaaatataa caagcagggt ataaagagac taaataacta ttttaggagg
      721 agtacggtat gtcattgaaa tcactaaaaa taaaagaaaa tttatattgg gtaggttcac
      781 ttgaccctga tttaagagta tttgacataa ttatgtatac accttatggg actacttata
      841 attcatatgt attaaaagga acagaaaaaa cagttttatt tgagacagtt aaagataagc
      901 attttgacaa ttatattgaa agattaaatg atttaaatat agattttgaa aaaatagact
      961 atattgttgt aagtcatact gaaccagacc atgcaggaag tgttgaaaag cttttggatt
     1021 tagctaaaaa tgcaaaagta gtagcttcag aaacagcaat taaatatctt aaggaaatcg
     1081 taaacaaaga ctttgaatat gttgcagtta cagatggaga tacattatca attggggaca
     1141 aaactttaga atttttctct gtacctatgc ttcattggcc agataccata tacacatata
     1201 taaaagagga taaaactctt gtaacatgtg attcatttgg tagtcactat agtaatgata
     1261 agatagtaaa tacacttgat gaaaatgaag aaaaggatta cttggatgct ttaagatact
     1321 attatgattg tataatgggt ccatttaaac catctatggt aactgctata gaaaagataa
     1381 aagatttaga tatagatact gtatgtccag gtcatggtcc agtattaact gaaaatccta
     1441 gaaaaattat agacctttat tacaactgga gtgtaaatga acaaataaag ttagaaaaag
     1501 aagttacaat ttgttatgtt tctgcacatg gatatacaaa gattatggca gaagctataa
     1561 aagcatatat tgaaaaaaat agcaactata aagtaaattt atttgatgta atagagcata
     1621 agcaagaaga tattttggca aaaatagcag tatcacaagg tgtattattt ggaacaccaa
     1681 caatattagg agatgcgtta aagcctatat gggatatatt aatatcgtta aacccagttc
     1741 ttcatggtgg aaaagttgct tcagtatttg gttcatatgg atggagtgga gaaggtatag
     1801 aaaatgctat ggagagaata agtcagctta gaatgacagc agtaaaacct tttgctgtta
     1861 actttaaacc atcaaatgaa gagattgata aattacattc ttatacaggt aaattcctag
     1921 ataagttaaa ttctactttt ggaagtaaaa agaaaactaa gaaatttaaa tgtgtaattt
     1981 gtaatgaagt ttttgaaggt gatagtgctc ctagtgtatg ccctgtatgt ggagctaagg
     2041 aagaccaatt tatagaagtt gaagaagatg aagttacttt tagaaaagat actgatgaat
     2101 atttcgtaat agtaggtaat ggagcagctg gtttttatgc agcagatgct ataagaaaaa
     2161 gaaataaaac ttgtaagata actatgattt ctaatgaaga tgagttgact tactatagac
     2221 ctgcactttc tgatggtata aatgaagaac ttggttcaga tttctacatg gaagataaag
     2281 attggtatga taaaaataat atagtagtaa tattaggaac gaatgtagat aaattagatg
     2341 aagtgaataa aactattata gtgaatgatg gagctattaa atttgataag cttgtaatag
     2401 ctacaggaag tagaaacttt ataccaccaa ttaagggcca tgatttagaa aatgtgttta
     2461 ctcttagaaa tataaaagat ttatatagtg taaaagaagc actagaaaaa tctaagaaag
     2521 ttgtagtgat tggtggcggt ttattgggac ttgaagctgc atgggaattt agactaaaag
     2581 gattagaagt agtagtagtt gaagccatgg atagcatact atcaaaacaa cttgataaag
     2641 aaggtagtaa aatattagaa caatgtgtaa gagatacagg aatagatgta agattgggtg
     2701 tagcagttga tggtattgaa ggtgatggaa aagctcaaaa agttgtattt aaagatggag
     2761 atagtgtcga ctgtgatatg gtagtatttt caattggtgt aagagcaaac actcaaatgg
     2821 ttcaggatac atctgtaaaa attgatagag gtattgttgt agataagaca ttacagacaa
     2881 atgttaaaga tatatatgct tgtggagatg ttgcacaagt gggaaatatt agtttagcaa
     2941 tatggccatc ttcagtagaa atgggaaaaa tagcaggggc aaatgcaagt ggagataatt
     3001 tgacatttga aagtgaagta tatccagttt cattagatgc aatgaatgta aaagtattta
     3061 gtattggtaa tatacaaaat tttgataaag aaatttcatc aaaagatgaa ggtcaaagaa
     3121 tatataaaaa attatttatg aaagatggtt cattagttgg agctatactt ataaatgatt
     3181 taagctgtac tgttaaattg ataagattaa tatctgaaaa aggtgatttt gaggatatta
     3241 tgaaggctga tatactataa tttaaataca ttaaaataaa aaggagttgt cttaaaattg
     3301 atttttttct aatcaaattt taaattcaac tccttttttg aatttttata taaaataaca
     3361 tggaaatttt agtaatgtct gtggttacat acataggttt aattgttttt tatatggatt
     3421 gtctctggta taataaattg aattgtgttt gactgataga tatttttatt gaaagttaat
     3481 acctgttatg cgataataag gacgtgttag gggagttttt ataataacaa ttaatctcag
     3541 tttttgtgtt ccaatagtag catcaacaca ttctgatgta gtagttcttt tcttataaaa
     3601 attagttaca aatatgcttg taattatttg atatgtaaat ttaccaatat tataaatgat
     3661 gacctcgata ataaggagag tgaaaaataa tctatgaacg aaaatatttt gattgtggat
     3721 gatgaaaaag aaatagcaga tttaattgaa gtatatttaa aaaatgaagg ttttacagta
     3781 tacaagtttt atacaggtaa ggaagctcta gaatgtattg aatctaaaaa tttggatatg
     3841 gctattttag atattatgct tcctgatatt gatggatttc atatttgcca gaaaattcga
     3901 gaaaaatact attatcctat cattatgtta acagctaaaa ttgaagatat agacaagata
     3961 atgggtttaa ccataggtgc tgatgattat ataacaaagc catttaaccc tttggaagtt
     4021 gttgcaagag taaagacaca attaagaaga tatatgcgat ataataattc ttatgaacaa
     4081 cagtcgataa ttgttaatga gtatgatatt aaaggattga ttatcaataa agaaacacat
     4141 aaatgtagtt tatttgggaa agaagtagca ttaacaccga ttgaattttc tattctttgg
     4201 tatttatgtg aacatcaagg aaaagtggtt ccttctgaag aattgtttga agctgtatgg
     4261 ggagaaaaat atcttgataa taataacaca gttatggctc atatagggcg attacgtgaa
     4321 aaaattcatg aaccttctaa aaacccgaaa tttataaaaa ctgtatgggg agtaggttat
     4381 acaattgaaa agtaaaaata ttaaagtcaa tcctgacttt actcaagtga gaaataaaat
     4441 ttttatgaga acagctttaa tggcatttat agccattatt ttcatatgga ttttatattc
     4501 ttttatattg tatggtaagt tttctatctg gatagtatca ttctttgaaa atgcttttcg
     4561 tatggaatat aaagctgcat tgaatttata tcagcagatt ttccgtaata gcatggagct
     4621 cttcatgata ctagctatgg tgattgtgtt ttttataata ttccgtgttt atttaaattg
     4681 gtttacaaaa tattttagtg aaataaatca aggtattgac tctcttatca aagaagatgt
     4741 tggagaagtg gctttatctc cagagttatt ggcaatagaa aagaaaataa actcaattaa
     4801 acatatactt gaacaacgta aatttgagac acaaatggca gaacaacgta agaatgaact
     4861 tattgtttat cttgcacatg atttaaaaac accacttaca tcagttatag ggtatctgac
     4921 actacttcga gatgaaagtc agatttctga agaactgcga aaaaaatatt tatctatttc
     4981 gttggataag gcagagcgtc ttgaagattt aattaatgaa ttctttgaaa ttacaaggtt
     5041 taatttatca aatatatcac ttgaatacag tacagtaagc ttaactcgta tgcttgaaca
     5101 actaacatat gagtttaaac ctactttgat gagtaagaat ttaaaatata cactgaatgt
     5161 tacaccaaat atgatgataa ggtgtgatgt taataaaatg caacgtgtat ttgataactt
     5221 attacgaaat gctgtttatt atagttttga agatactact attgaaataa cagctgtaca
     5281 ggaggatgat tatataaagc ttaaatttat gaatcatagc aatacgattc cagaggaaaa
     5341 acttgagcgt atttttgaac aattttacag gttggataca tctcgtaatt caaacagtgg
     5401 tagagctggg ttgggacttg ctatagctaa agaaatagtg gagcttcata acgggacaat
     5461 aacagctcat agcgagaaca atataattac ctttaaggtt attattccga ttgtaggaaa
     5521 tttgtaagaa tttaagtata aaaaaatagg aaattcttta ggataaataa ataaagagca
     5581 ctcttgttat tgatataatt actgtatcaa aacaagagtg cttttatttt gatacagtaa
     5641 ttatatcaat agaaaaatgt gaaaatatat tatgtggaac agatgcaatt gtaagtgaag
     5701 gttgattgga tttgaaatag aaaaagtatt ttaacatgtt tctgtagatt gtataaaaat
     5761 aaataaatca acaaaggaga atcaaagatg aataataaaa aaattgcagt tatatttggt
     5821 ggaaactcaa cagaatatga tgtatcatta caatcagctt ttgcagtact tgagaatttg
     5881 aatacggaaa aatatgatat tataccaatt ggaattacaa gggagggtaa ttggtatcat
     5941 tatattggag attataataa tatccagaat aacacttggt ttgaaaggga tactgatata
     6001 attccagtta ttgtttcgca gaaccgtgtc aaaaatggta ttattgaatt tttaattcct
     6061 gatgctgttt ctcagaatca tattccaaat agtgctattg aatcatgtga aaatgatatt
     6121 aaatacacta aacttgattt tgtgtttcct gtactgcatg gaaaaaatgg agaagatgga
     6181 acagtgcagg ggttatttga attggcaggt atacaggtta ttggatgtgg tactctttct
     6241 tcagcattat gtatggacaa agacaaatca cataaacttg ttcattcaga aggtattaaa
     6301 gtaccaaaat caatattact aaaatactca gataaattta atgtggctga actgtcaaaa
     6361 gacttaactt atccattatt tataaaacct gtgcgtgcag gttcatcatt tggtattagt
     6421 aaaatttata aaaaagaaga gttacaagta gcagtagact tggcttttga acatgataat
     6481 gaagtaataa ttgaggaaaa tattgatggt tttgaggtag ggtgtgcagt tttaggaaac
     6541 gatgacttac ttgttggaag agtagatgaa atagaattgt caaaaggatt ttttgattat
     6601 acagaaaaat atacattgaa aagttcaaaa attcatatgc cagctagaat tgatttggat
     6661 tgtgagaaaa gaatacaaga gacagcaaaa attatttata gaactcttgg atgttctgga
     6721 tttgctagag tagatatgtt tttaactcca tctggtgaaa ttatttttaa tgaagtcaat
     6781 acaattcctg gctttacttc tcatagtcgt tatccaaata tgatgaaagg tattggactg
     6841 tcttttgaag aaatattgga caagataatt gaggtgtaca taaaatgatg aataatattt
     6901 tattagataa aaataaagaa gataaaatga caaatactat cttattgact aaagataaag
     6961 tttatgatgg gaatctaatt ctagtaaatg cttttttacc agttaaaaca tcagaagata
     7021 tagatttaat tcctgtagat acaaggtttc caagtattct tatgaaacgt gaagctacaa
     7081 acattttaca aaatatttta aaaagtatat gtggaataaa tgaaatagta cctgtaagtg
     7141 gatatcgtac tgcagaggaa caacaagata tatattcaag ttctctgcgt gacaatggaa
     7201 aagactttac taaaaagttt gtagcacttc caaatcacag tgaacatcag acagggcttg
     7261 ctattgattt agggttaaag aaagaagtta tagattttat ctgccctgaa tttccttata
     7321 atggtatttg taatgagttt cgtaaggcag cactacatta tggttttata gagcgttatc
     7381 agtgtaaaaa agaagaaata acaggaatat ctcaagaacc atggcatttt cgttatgtgg
     7441 gatatccgca ttctgagatt atcaataaaa tgaatctcgc tcttgaagaa tatatagaat
     7501 atattaagag attttcgtat acaaattgtt taaaaataga agataaagaa aaaattatag
     7561 aaatctttta tgtgcctatc catactaaag aagaagtgag aatctctata cctgaacaat
     7621 gtgtatatca aatatcgggt aacaataagg atggttttat tgtgacattt tggagaaata
     7681 gaaaggacta agaaatggag gaacaagaat gattaggaat aaatcttatg cagggcttga
     7741 ttattttagg attatagctg cattacttat aattgcaatt catacatctc cattattatc
     7801 atatagtaaa atggctgact ttgtgttaac tagtataatt gctcgtattg ctgtgccatt
     7861 cttttttatg ctatcaggct tttttctaat ttcaacatat aattataatt gtaataaact
     7921 gaaatctttt ctaaaaaatg tattatttat ttacgggata gctattgtaa tttatattcc
     7981 cttaaatatt tacactggat atttttccat gaataattta gtgcccaata ttattaagga
     8041 cattgtattt gatgggactt tatatcattt atggtatcta cccgcatcaa ttattggtgc
     8101 aagtattgca tactttcttg taaaaaaact aggtttaaag tactcactag taatcactat
     8161 tgttatttat gtaattggta tgtttggtga tagttattat ggttttttag aaaaaatacc
     8221 actattaaag aatttatatg ggtatatttt tgaagtatca gattatacac gaaatggtat
     8281 attttttgca cctgtatttt tcatacttgg tggaataata tctaataaat ctaattatat
     8341 gtcaactaaa aatagcttga ttggatttct tatatcattc ttacttatgt tatgtgaggg
     8401 aatgttttta cataaactag gggtacagcg tcatgatagc atgtatatta tgttattgcc
     8461 atgtatgtat ttcttattta cagccttaac atttttgaaa ggttctcgag ctaggttatt
     8521 gcgaacatca tcattaatta tttatattat acatcctatg atgattgttg ttattcgtat
     8581 gttttctaaa atattcggaa tacaaactgt cttggttgac aatagcctta ttcattatat
     8641 agtggtaagt atagcatctt ttatagcttc tataattatt atattgattc ttaaacatct
     8701 aaaaaaagac ttgaaaggag aacataaggt aaataccaat agagcttgga ttgaaattga
     8761 tttaaataat ttggaacata atgtaaatat gatacaaaaa gctatgccag aagattgtga
     8821 attgatggca gttgtaaaag ctaatgccta tggtcatagt gcttttgaag tagcgacttg
     8881 tgcaaaccaa ataggtgtaa aagcatttgc agtagcaaca atagatgagg ggattgattt
     8941 acgtcgatat ggaatactag gagaaatatt gattcttgga tatactaacc caataagggc
     9001 aaaggaactt cataaataca atcttacaca gagtgttatt gaccttaatc atgctatctc
     9061 tttaaacaaa cagaaataca acataaaatc acacattaaa attgatacag gtatgcacag
     9121 actgggtttt gatgttaatg atgtgaggtc aattttaaag gcatttaaat taaaatactt
     9181 agatatatgt ggcatattta cacacttgtg tgtttcagat agtctgaaag atgaagatgt
     9241 agactttaca aataaacaaa ttgaatgttt ttataagtta attgattttc tgttaaaaaa
     9301 aggtataaaa attcctaaag tacatattca aagtagttat ggttttttta attatcccaa
     9361 tcttaagtgt aattatgtaa gaattggaat tgcattatac gggatattaa gttctccaaa
     9421 tgatacgact aaattgcaac ttgatttacg acctgtttta tccttaaaat cacaagtaat
     9481 tcttattaga aaaatacaaa aagatgatag ttttggatat ggaagagtct ttattgcaag
     9541 cagagatagc gtaattgcta tattatctat tggctatgcc gatggtatac ctagaaattt
     9601 atcctgtgga aaaagttacg taataataaa tgattgtaga gcagcaatag tgggtcgaat
     9661 ttgcatggac caacttgctg ttgatattac agatatacct aatgtagaag ttggtaatac
     9721 ggcaataata ataggaagag ataatttagc tgaattatct gcttctgaag ttgcaaataa
     9781 ctcatgtagt atatcaaatg aattattgag cagagtagga agaaggttaa atgttataaa
     9841 gaagtaactc aattaaatag taatatttgt tatactgtgt aatttatttt actctgacta
     9901 gacattttta gtaatattat gtctaatata tattttttat aacttacatg acaatttaag
     9961 ttataaaaag gtgatgtttc agatttttat tttgaaacat cacctttttt gttataatat
    10021 ataataaaat aattatacct taactgtaaa cttatataga ttatttactt tttaatatta
    10081 gattcatttt ttgagattgg tatataataa acatatagag tatataataa tagttaaagt
    10141 taaaaaaggg gaaaagcaat atgcagtata tacaagaact acttgatttg actcaaaatt
    10201 attgggtgtt agccacaata atcggcttat taagtgcatt tatagaaagc tttataccag
    10261 ccttaccact tgttgctata gttacagcaa atgctgcaat acaaggatta tttattggat
    10321 gtttgttatc atggattggc tcaggacttg ggacaacttc attattctta ttaataagca
    10381 gatttaatga tagtaagctt tttaataagt tgagaaatag taaaactgaa aaagcaataa
    10441 gttggatgga taaacaggga tttaagttat tatttatagc gtatgcctgt ccatttatgc
    10501 ctggttgttt agttactatc gcatcagctt ttggtaaaaa agatttaaaa gattttgtgc
    10561 cagcaatgtt ggcaggaaaa tttgtaatgt ttatagttat aagttacgtg gcaagtgata
    10621 tagaaggttt tataacaagt cctttaaaga tagcatcatt tatattattg gtgtttttgt
    10681 catggaagat tggaaataga gtaaataaaa atcttgaaaa tcataattat gatttccatc
    10741 ataaaaaaca tcacaatgat aaagatgata aaatgattta gcttgtacga aataaaacgt
    10801 atcttaaaat gatttagtac taaattattt taagatacgt tttatttttt gtattaaggt
    10861 acaaaatttt tagttctttt gtaaactaaa atatataaaa cttatttata cttagttcaa
    10921 aatattgtta tt
//
LOCUS       DAGSRM010000223        10805 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.guided.119, whole genome shotgun sequence.
ACCESSION   DAGSRM010000223 DAGSRM010000000
VERSION     DAGSRM010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10805
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.guided.119"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(136..954)
                     /locus_tag="MJE33_003702"
     CDS             complement(136..954)
                     /locus_tag="MJE33_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBY9649569.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(947..1645)
                     /gene="cbiQ"
                     /locus_tag="MJE33_003703"
     CDS             complement(947..1645)
                     /gene="cbiQ"
                     /locus_tag="MJE33_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HBY9649570.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEFFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(1648..1926)
                     /locus_tag="MJE33_003704"
     CDS             complement(1648..1926)
                     /locus_tag="MJE33_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HBY9649571.1"
                     /translation="MSAKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEITK
                     INPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(1916..2668)
                     /locus_tag="MJE33_003705"
     CDS             complement(1916..2668)
                     /locus_tag="MJE33_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HBY9649572.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAIPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(2850..3042)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(3174..4946)
                     /locus_tag="MJE33_003706"
     CDS             complement(3174..4946)
                     /locus_tag="MJE33_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HBY9649573.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDIILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(4960..5889)
                     /locus_tag="MJE33_003707"
     CDS             complement(4960..5889)
                     /locus_tag="MJE33_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBY9649574.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            6117..6305
                     /locus_tag="MJE33_003708"
     CDS             6117..6305
                     /locus_tag="MJE33_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY9649575.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRTKRRKEVQYE"
     gene            6298..6999
                     /locus_tag="MJE33_003709"
     CDS             6298..6999
                     /locus_tag="MJE33_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBY9649576.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAIIAHCGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGQVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            7001..7924
                     /locus_tag="MJE33_003710"
     CDS             7001..7924
                     /locus_tag="MJE33_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBY9649577.1"
                     /translation="MNTSVYLLLILVLSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            8081..9004
                     /locus_tag="MJE33_003711"
     CDS             8081..9004
                     /locus_tag="MJE33_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBY9649578.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKEREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            8997..9734
                     /locus_tag="MJE33_003712"
     CDS             8997..9734
                     /locus_tag="MJE33_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9649579.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPIKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANIALGRRK"
     gene            9745..10518
                     /locus_tag="MJE33_003713"
     CDS             9745..10518
                     /locus_tag="MJE33_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBY9649580.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETNLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
BASE COUNT         3525 a         1801 c         1486 g         3993 t
ORIGIN      
        1 ttccatataa tttatttcat tgtttaataa aaaaaataac cttatgattt ggggaatcat
       61 aaggcacctt taaggggtct aggttaaggg ttattatata taaatctatt tttttaaaat
      121 aggtttaggg cttaattatt tatttgtatt aagttctctt tccttccaat acttatacaa
      181 atcttcttct ttcttaaata agggtaaatt catatttcta tgtactttta ctaaccatgg
      241 ggattctagg ccagcttgta taatattatt ttcatttata aacacatttt ctgcttttcc
      301 ttcatcaatc aaaatcccct tatccaatac atatatgtaa tcacatattt catacattaa
      361 attcatatca tgactggata ttactatttt tatattattt ttatttaatc cctttattat
      421 atctacaata gacctagtac ttactgggtc taaaccagct gtaggttcat caagtaatac
      481 aatttcattt tccattgcta taactgatgc tattgccact ctttttttct gtccatagct
      541 aagaaaatgt actggcctgt ctataaaatc tattccatta acagctacta gagctttgtt
      601 tattctctct ttaattgttt tttcatccat accaatgttt ctcattgcaa aggctatatc
      661 atcatatact tttgaataaa aaatttgttt ttctgggtct tggaagacaa tacctacatt
      721 ttttctaagg ttgtataaac ttctcttatc atattttaac ttttcttctt taaaaagaat
      781 ttctccagat gtaggcttta atatacccat taaattcata aataatgttg attttcctga
      841 accatttgaa cctattatac ctataacatt tccctttgaa aaatccatat taatgtttaa
      901 tagtgcattg gtattttttt catattgata cgttaaatta tttattttaa acatcattat
      961 cccctactat gtgaaattta ccatcataca attttatgtc taatgatata cacatatcat
     1021 cataccttgt cataagtctt ttgtacagca tacttccaag aatacctaag gatttatatg
     1081 aagtttttaa atttatatat ccaaatctta gctcttgtga cttgtatatt tctgaaaatt
     1141 cttctaaaaa tatgaatata aacctatata ttaacattga taactcgaca aaagtgtcag
     1201 gtaaatgaag ttttttaaaa aagaatatta attgattaaa aggtgttgta agtatacaaa
     1261 aatacacaca agtcaaacaa gacatcgctc taaataacac atgaatagac atgtcaatag
     1321 acgcctttga tattcctaca ttaaaactaa aaaattcaaa actatataat aaatctgctt
     1381 tattaaaaga tatatttaca agagttattc ctatactaag aaataaaaag tacattggta
     1441 ttctaagtaa ttttaaataa ttttttaaat ctattccagc cacacaaaca accaaaatac
     1501 tcattaaaat cataattcca cttaaaataa atatattttg tattaccata gaagcaatca
     1561 gaaaaattac tcctatggct acttttttgt ttggatttac tttactcaat ctatttgtat
     1621 atgcatattt atcaattata agcatgatta acttctcttt cctttttgaa gacctaatat
     1681 gtatcctatt acaccagccc ctaaagctgc ttgtgcagag aataataaac tttctatttc
     1741 tccacttggt ggctcatatg gactgctaaa ccatggtttg taatttggat ttatcttagt
     1801 aatttcactt tcagcttgac catctgctcc tccatattca gcacctgagt tgacaagtaa
     1861 tgggaatata ataagagcta ctactaataa aagtaataat atgttagttt ttgttttagc
     1921 actcattagc tttaactcct ttatcattat tttctagtat gcttttttct ccgttttctg
     1981 taattaaatt atatacaatt acagtaagta taccttctgc tattgctatt ggtatttgag
     2041 tcataaagaa aattcctaaa aatttaattg ctgaagccat taccccacct gatgggtctg
     2101 ggaaagccag tgctaattgt attgaagtta ttgtatatgt ggctaagtct cctattgctg
     2161 ctgcaagaaa tactggaata gcattatttc catctttttt ctttaaagcc ttaaatatta
     2221 ggaatgatac gattggccct attatcgcca ttgaaaaagc attagctcct aaagtagtta
     2281 ttcctccatg tgctaataac aatgcttgga atattaaaac tatagttcca agaacaaaca
     2341 ttacgcttgg tccaaacatt attgctccaa gacctacacc agtaggatgt gaacaacttc
     2401 ctgttactga aggtattttt agtgctgata atacaaacac aaatgctcca cataaagcta
     2461 ataatacttt ctttttaggg tcttgcttta ctattttacc tattgatttt aatccgaata
     2521 agaaaaatgg tataaatata actccccatg ctatagacca ttttactggt aagtaacctt
     2581 ccattatatg cattgcattt gatgtaactg gagttaaaat gattaacata agtgcagcta
     2641 taacccctaa tttaatattc tgtttcatct tagtatccct ctctcccttt ctaataatta
     2701 aaacataata atacatgtct gatttattta ataaaataaa aaagcctaac tcgatggtta
     2761 ggcttctaat tttccacaat ctaaatacaa acacataaaa caaacatatc tgtataatta
     2821 ttataagaaa ctttctccat ccccgaagaa attactttaa tattttaggc aggtctcctg
     2881 acttagcttc atcctactcc tcttccttcc catgagatat aaaaaatatc atcatagtgg
     2941 ctaacaaaag tttcgtctgc atcacagtag cgggggctgt aaaggaattt aacctttttc
     3001 cctattaatc ttgcttaaag aacctataat attatatatt taatttttaa attcttctta
     3061 aattattttc tatataaatc attgtatacc ttttctgtga aaatttctac attttttata
     3121 aattttattc atttttatat ttttatgtat ataaattctt ttttgtaagt ttattaattt
     3181 ctaattatct tctttaaata cattgaaact acatcagaaa atctagttat atctcttgaa
     3241 tatataaaat catttccata tattaatctt tcagcttcaa gttctgattc ttttcctgta
     3301 aatactccta atactaatat attttgcttt ctagcttttc tcacttcatt ggctgtatct
     3361 ctcaaaccaa ctatacccct ataagccttt tctcctctta atgttctctc actacttttt
     3421 cctatcttta catcatttgg tcttccatcg ctgagaacta tcaggatttt attttcttca
     3481 tctctattta ttagaccact acatatagat tttattgcca aaccatctct attattacca
     3541 gcacaaaaat attcaaatat attttcacta ttttttatac tatcatcata atctttaaat
     3601 ctttttaata ttgtatagtc taaaaaacta gaaaatccca tcactctatt tggtatgcca
     3661 acttctgtta gagcatttgc aattatgtaa gcttgaattg ccacatttcc ctgatttcta
     3721 ctttgagagc cacttgcatc caataatata tcaataacgt atttcccttt ttcattttct
     3781 atatctttat aaaatatttt attattatta cttctaccaa ctctccaagc tctatttgct
     3841 aatatagtac cacaatcaga atatactcta cttttagcat tttcttctat taaaattctt
     3901 gatagacttt cttttaatct ctttatttgc tgtctataca ttctagcatg ttctctaaat
     3961 tttcctaaat tattttcttt ttgtcttgtt acatatttta gttgaaatac attattacac
     4021 tcacttctaa gaactccatc tgtaaagtgt attctacaac cttcatgaac atttctacaa
     4081 ttttgtgtct caattttttt aatttcacct ttactcaaat aagtttttcc ataataatgt
     4141 tctatttttg aatatatctt atttgcagtg tcttcatcaa catatataac tcggtctgct
     4201 gaattgctac atttagaatc atcaaaatcc cctacacctt ctacaagcat ggatgaagat
     4261 attgaatcta tatcactttc aataatattt tcttcttcat cactataaag ttcttcatac
     4321 atgaaatcag caaatgtatc aaaatttact tctacacttt ctacattatt ttcatcaata
     4381 agactatttt cttctccatt taatatgtat tcaaaataag ttaaatatat aacatctaat
     4441 gcttttaaaa tctccataat atcttgattg gtatctatat ttcttatatc attaactatt
     4501 cttctagtca tactatctaa tgctgggtgc ttgtccatac tataaagtac aaatgtgtat
     4561 ttaattttat ccataatatt atcattgtgt attttggtaa aacgtgaaac aatatcttta
     4621 taagcatttc ttcttatgtc ttctatccct ggcctatctt taatgacctt ttcttcaact
     4681 attgtattta atataacttg aattagaccc agtataatat ctttgttgta tccttttctt
     4741 attctatttg taagatattt tttaactata tcccaatcta catactttct tctagcacct
     4801 gcaattattg caaaatatag acctatttct tttgaagagt aatctttttc tgatatatct
     4861 atactttcat tgtaatcacc agctattgtc catgataagt tattagctct attttcaaat
     4921 tcataatcat catatatcca tttcataatc tcacctatat taaatatgac ttattggaaa
     4981 cacatctata ctctcccatt tattaggtat tctagtttta attacatctc caaccatttc
     5041 tttctcatat acatcaaaag tctttcctgt aagtcccata ttaattgcaa gagttggttt
     5101 taatcctctt cttatagtct taagagatgc cataagacca cgtaaatcaa tagctttact
     5161 agatatctct ccattttgag acttcaattg taaatctaaa aatattcctg caaagtgtat
     5221 tagtttttct tcatcagcat ctgaaaattc attttttaaa atcatcatta acttatcttc
     5281 ttctatagga ggaatatcta ttaccataaa tctagatacc agtgcttcat taagttcttt
     5341 tgttcctgca tattcataat tcatagtccc aataaatctt gttgctggat gtagattgac
     5401 tctctcatac ccaggtacat caattattct tctatagtct aaagctgaat gtaccacaac
     5461 tatagcatca ttttttgcca tattaatttc atcaaacacc ccaaaacctc cattaatagc
     5521 acactcataa actgagcctc ttctaagttt aacttcattg tctataaatg tatctgtacc
     5581 tataagtgtt gaactgtctg tatttatatg aaatgatgta ttccactgag gtctattaaa
     5641 tatttcccct agattatcag caagtaagtt ttttcccgtt gcctttggtc ccgataataa
     5701 tatattttct tcttctaaaa tagctgctat acacatagac aagatatctt taccataaaa
     5761 ataagtttta gactttgtta ctctgtattc taaatctttt tctaatccat aataatttct
     5821 aaaatacagt acatcttcaa taagtttttc atctacccct tgcaccctta gattatctat
     5881 tatatccata tttttacccc ttttatataa atagtatttg tttcatttct tatcaccatt
     5941 ttagcataaa aggatataag ctaaaattga ttttttaatc tccccatttt aagtaaaacc
     6001 atgcaccatt ttatcactac aaaattaaga gtactactta ctgatatttc ttgattttgt
     6061 tcgtctatac gtatataata tatgctgata aattatgagg aaaggatgat tgaaatatgg
     6121 aatatatgtc ttgcccagaa gtagcaaaaa aatggggtat ttctgaaaga agagtacaaa
     6181 aactatgtga agaaagtcgc ataccaggag tttcaaaact cgggtatatg tggctgatac
     6241 caaagaacgc agaaaagcct gttgatggta gaacaaaaag aagaaaggaa gtacaatatg
     6301 aatagcgttt tgattataga tgatgacaaa gaactttgtg ctcttatgaa aaaatgtgtt
     6361 gagcaagaaa atttatctgc tattattgca cattgtggtg cagaaggtct acatttggtc
     6421 gataaaaata gacacacact ctctcttgta attttggaca taatgatgcc agatatagac
     6481 ggttttcaag ttctgaaaaa aattagagaa acaagtaata ttccagtaat tatgcttact
     6541 gctaaaaatg acgaggatga taaagtttcc ggactacgtt taggtgctga cgattaccta
     6601 acaaaaccgt tcagcataaa cgaacttatg gctcgtgtca attctcttat tcgtcggtat
     6661 actatgttaa accctgcttt tataacagac acagattgca taataataaa tggcatgatt
     6721 attgacaaga caaaccgaat tgtttctgtc aataatattc ctgttcaact tacaagcaaa
     6781 gaatttgatt tacttgcttt tctggcttct aataagggac aggtgtttac caaaaaacag
     6841 atttatacac aagtatggga agaagaatat gcttttgacg atagtaacat catgtctttc
     6901 atcagtaaat tgagaaaaaa gattgaacca gaccccaacc atccatttta tattttgaca
     6961 gttcgtggtg tcggttatcg ttttaataag gaggcttaac atgaatacaa gtgtctatct
     7021 gcttttgata ttggttctta gcctgattat tatagcgtac cttgtttctg tcctgctacg
     7081 tgtccgaata caacttacac ttataaaaga tgctttagag gatataaaaa atggaaacct
     7141 taatcgacgt attttaacag atgaaaacga cattacaaaa caaatttgct atgatattaa
     7201 cgaaatagca atcaatagcc aatttcaact tattcagcaa aaacaatcag aacaagccta
     7261 caaacagcta atgacaagtc tctcccatga tgtaaaaact ccacttgctt ctttggtcgg
     7321 ttatttggaa gcagtagaaa gtaaaattgt tgtaggagag gaaaaagacg agtatatcca
     7381 tgttgcgtct aataaggctc attacttaaa gaattttgta gaaaatctat ttgaatgggt
     7441 aaaattggat tctaaggaac aagtttttca ttttgatatt tttgacttga atgaattatc
     7501 ccgtaatatt atatctgatt ggatacctgt tttagaaagc agtcattttg aatatgaatt
     7561 tgatatacca gaaatagagt acttcttacg tatagacgca aatgcatata cccgtatcat
     7621 taataaccta ttacaaaata ttattaccca tagtagtggc aacaaaatga cattgagaat
     7681 ttttgaaaac aaagagcaag ctcaaattat aataacagat aatgggaaag gtatatcttc
     7741 agacaatctc ccacatattt ttgaaaggtt gtaccaatgc gaccattcac gagcttcaaa
     7801 gggaaatggt ttaggcttgg cgattgcaaa ggaacttatt aacgctcata agggaaatat
     7861 taccgccaac agcactcccg gtatgggaac agaatttaca atattgcttc ctaaagccct
     7921 gtaaaagtac gaggctttct tactctaaaa caagataaaa gcaagattat ggcaaggttt
     7981 tggcaaggtt catgttttat aattctaatt gtagttgatt ttgacgttgt atcaactatc
     8041 ttttgtagaa acaaatttat taaaagtgag gaaattatat atgaatgatt ttgtgattga
     8101 aacaaagcaa cttacaaaaa tttatgggaa tcaaacagta gttgacaaag tcaatctcca
     8161 tgtaaaaaaa ggccgaatat atggtctgtt aggacgcaac ggagctggga aaaccacaat
     8221 tatgaaaatg attttaggat tgacttcaat cacgtatggc gaggtagatg tatttggaga
     8281 gaatattaag gaacgtgaaa aacaagtata tccccgtatc ggtgcaatta tcgaaacgcc
     8341 aggattttat cctaatctga caggaacgga gaacttggag atttttgcaa aactgcgtgg
     8401 tacagccgcc cctaattctg taaaaagagc attggaaatt gtaggcttac cttataaaaa
     8461 taaaaagctg tttagtaaat attcacttgg catgaaacag cgacttggta ttgccaacgc
     8521 tatcctgcat gacccagaac tgttagtttt agatgagcca accaatggtc ttgaccctat
     8581 tggtattgcg gagatgagaa agtttattag ggacttgagc gttgagcatg gaaagacaat
     8641 tcttatttcc agtcatattc tttcagaaat ctcattgttg gctgatgata ttggaattat
     8701 cgaccacggt gttcttttgg aagaaagtag tatgagtgaa ctagaaaaga aaaacagtaa
     8761 atatatcctg ctacaagttt cagatattcc gaagacatca cttattttag agcgtcaatt
     8821 taacacgaag gattattccg ttcaagatga ctacacgtta cacctttata acactaatct
     8881 ggatatgggt gaaatcaata aagcacttgt aatgcagaat gtaactgtta ttagttctca
     8941 actttgtaat gatactttag aggactactt taaaaaaatc acaggaggag agggcattgc
     9001 ttaaacttat tcaaacagaa tttcttaaac tgcgtcgtag gaaacttatt tggctgatgt
     9061 tgttgtctgc tctcattatg ccgtttttcg cactactata cttcaattat tttggtaaaa
     9121 caggtgttga gccaataaag ttttataaat ggtctgcatt tggttataca ctttggatta
     9181 ttttgcctgt tgttttgggt atattttgca ccatgcttat acacgaagaa aaccagtatg
     9241 atatgctcaa acagctttgg attgtaccta ttagtaaaat gggatacttt tttagtaaat
     9301 ttttcgttgt tttgatttac tctatttgtt ttatgctgat tacagccgtt gcttctgtgt
     9361 tatttagtgt actttctgga tatgtcgtat ttagctgggc aagcatttta tatttgttga
     9421 aaaaatgttt agaaattggt gtaatcactg cttttgtaat gttgccgatt ttggcaatcg
     9481 ctacatctca aaaaggatac attctcccaa tttgtatcac attggtatat gccttttccg
     9541 gttttgttat aatgacaata aatatgtatt tacacccatt atccagtatg ggagttatta
     9601 tcatgagaaa taaagacatt ccaggtctta tattttcaca ggaaattaat gtcccattag
     9661 catttctttg tatttgtatt tggagtattg tatcagtatt gtttgcaaat attgcactag
     9721 gaagaagaaa gtgaggtgta gataatgtgg aaattacttt gggctgaatg tcaaaaaatt
     9781 cgtcgttcaa aaattgtttg gattacagtt tttgcatcca ttatgatagc agtcatagtt
     9841 tttgcagggg ggcaggatgt atataatgac ccagatttac attatggctt aaagagcgtt
     9901 tatgacggtt cacggtatat agacaatgca ggttggtata tggacgcagt acaaccttgg
     9961 tcaacttttt ttgttcttcc tgctgtcatt gccctattag gaagctatat tatttgtcgt
    10021 gaagaagagg aggacactat taaatccctg cgtcttattc ccataaatga aacaaattta
    10081 accatagcaa aaatgattat tacttttata ttcagtatac ttctttattt gttactcttt
    10141 actataacat ttttgactga atccgtttta catttttccg atttatcgac aaaattggtt
    10201 ttaagttgca tgaaagagta ttttcttgat ggcataggtg tattctttgc aatttctccc
    10261 attattgcct tggtatcgcg catgaaaaaa ggatattggc ttgcattagt attcacagaa
    10321 gtttattcta ttgctggact atttgcgggt atgtcaaatg tcttacaaac cttttttcct
    10381 attgcggcaa tatttaatat ttctggatac tatataacta ctggagaaaa aattatgggg
    10441 agtatcataa gtttattact ttgtgcctgc ctttctgcat ttatacttaa agggctaaaa
    10501 cacaatgaaa aaaattgaaa taggtatatc aaaatgaaaa agataaaaaa actgattatt
    10561 ctaattgtat tcttgtttct ttttggcatg ttacactgtt atactgaatg gaaattcaac
    10621 aataacccac ctaaaggggt aatagaaaga ataaatgagt ggactggatt agtaacttat
    10681 gttaattata gtgaatgtaa tattagcaag ttattgtttc gtattttaat actataactt
    10741 taaatatttg gttttaatct tccaaacgaa tataaaaaaa ctgtcacaga ggcaacatat
    10801 ttttg
//
LOCUS       DAGSRM010000224         9698 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.guided.125, whole genome shotgun sequence.
ACCESSION   DAGSRM010000224 DAGSRM010000000
VERSION     DAGSRM010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9698
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.guided.125"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            complement(168..1361)
                     /locus_tag="MJE33_003714"
     CDS             complement(168..1361)
                     /locus_tag="MJE33_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBY9649581.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(1440..1646)
                     /locus_tag="MJE33_003715"
     CDS             complement(1440..1646)
                     /locus_tag="MJE33_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBY9649582.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            complement(1639..2781)
                     /locus_tag="MJE33_003716"
     CDS             complement(1639..2781)
                     /locus_tag="MJE33_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBY9649583.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            3290..3925
                     /locus_tag="MJE33_003717"
     CDS             3290..3925
                     /locus_tag="MJE33_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBY9649584.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGTNIEASDLYLIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNHFDDEDE"
     gene            4416..5348
                     /locus_tag="MJE33_003718"
     CDS             4416..5348
                     /locus_tag="MJE33_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBY9649585.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTEELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5593..5709
                     /locus_tag="MJE33_003719"
     CDS             5593..5709
                     /locus_tag="MJE33_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBY9649586.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5725..7644
                     /gene="tet(M)"
                     /locus_tag="MJE33_003720"
     CDS             5725..7644
                     /gene="tet(M)"
                     /locus_tag="MJE33_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBY9649587.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGIVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            7763..7930
                     /locus_tag="MJE33_003721"
     CDS             7763..7930
                     /locus_tag="MJE33_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBY9649588.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(7990..8343)
                     /locus_tag="MJE33_003722"
     CDS             complement(7990..8343)
                     /locus_tag="MJE33_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY9649589.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            8848..9267
                     /locus_tag="MJE33_003723"
     CDS             8848..9267
                     /locus_tag="MJE33_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBY9649590.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSCDCIFFKVLNYTIQVSNEKLAVALYKLSSKERDVILLRYFQ
                     SMSDQEIAELYHVSRSAIYRRRSNGLKKLKIVLKERN"
     gene            9309..9512
                     /locus_tag="MJE33_003724"
     CDS             9309..9512
                     /locus_tag="MJE33_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018595044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY9649591.1"
                     /translation="MCQASAGSETAIREILKFYDAYICKLCLRPFYHSESGKITMQVD
                     EELKGQIHTEMMKAILKFEIRVK"
BASE COUNT         3044 a         1720 c         1870 g         3064 t
ORIGIN      
        1 aaattatata tgtctttttg actatctttt tatatcttct caaatgtccg caaagcctta
       61 ttttatcggc tctacgggca ttttgctttt gtggtaaacc tcgcatatct aggtctatct
      121 tcttatattt tccctgtcaa tcgtggttaa aatcgtggta aatacttcta tgcgattaga
      181 cgctgaacct ctgattttgc ggtatctatg gacgcatgag cgtaccagtt cattgtgata
      241 ctgatgtttg aatgccccat gatatactgt aaatctttcg ggttcatgtt cttgcttgcc
      301 agccttgtgc agaatgtatg gcgtagcgta tgcggtgtaa tatgtggcaa gggattgtcc
      361 ttgtggtgct tgttgtattt ctttaccata cggataaata aggcgttata atcaatggca
      421 acttttggat tacctttcgg attgataaac acaaaaccgc tgtgtccgtc tacctctatc
      481 gactttatct ttggtcggct gttgataacc cgttgaaatg cctgtatcgt ttctttgctt
      541 aatggcactt gccttatccc gcttttcgtc ttaggcgttt caatataata gccctgttcc
      601 ttactcttta gtaactggtg gtcgataatc acaacttcat tcttgaaatc aatgtcggct
      661 actgtcagtc cgcacagttc cgagatacga agtcctgtct ttaacagtat cagcacatca
      721 tcataatgct tgtgatacac gttgtccgtc ttaataaatg acaataaggc ttgctcctgt
      781 tcctctgtca atgcgacttt ctctttggta tcattttcta tgacttcact tagcttgaaa
      841 tcaaaagggt ttttcctcac ataatcatct tgtatggcta tgtagaatga cgcttttaac
      901 gagcgtttat ggttgttaat ggtattgtag gaaaagcctt tgtctttcat gcgtaacgcc
      961 cattctttag cgtcagaggg ttttatcgta tcaatgctcc tagcacctaa cttgtcctct
     1021 tttaataacc gcatgagttg ttcccgttgt ttctgtgtgc ttttcttcac atttgtcctc
     1081 tgtgcgttct gtttggcata gagttggcaa agcgtcattt tcttgcctgt gctgtcgata
     1141 ccgtcctcaa tatcccgtct tatctgctgt tccagttctc gaagtgaggg tttttcccgt
     1201 tttccctttg gtgtcgggtc tgtgggtgtc aatctccaag catacacata ttttgtgttt
     1261 cccaatgcgt ctacatattt atataagtat tttccgtctg ttcgttggct ctctccagta
     1321 tgcaggatac gacctttgtt atcccgtctt ttttctttca tggtgtctgc tcctttcctt
     1381 gttggaaaga gccttgatat gacttgtgtt catcataaca catacaaggc tcatttgcat
     1441 tagattgcgt ccaatttatc aataaccttt tcaaactgtc ggcgtttaat ctgtatgcgg
     1501 ttgccattca taatgagcca gccagcgtcc ttgttttcct ctgccaatcg tcttaacttg
     1561 ttttctccaa tgcggaaata ctttgacgct tcttcaatgg taagggtgta tttttcccat
     1621 acgggaatat cctgtgtatt actcataaaa caccccctca ctttcgatta cgtctgatat
     1681 gtccgtacac atctgttcca caagcatttc ctgtttttca gaaaggtggg tacattcatc
     1741 aatcattttc atgtagtttg aacctgtcag cctgtcgatt tcctctatca tcacgattga
     1801 cggcatgacc tgtctttcca gccattgcag agtgcgttcc atattgacag gtacagggtt
     1861 catttcaaaa ggtaaactgt catgggaaat gaatatctcc cacaatggat agtcaggaaa
     1921 ttccacaaaa tcggtaatga gataaaacac cagcccatga gggttatacg tcaataacag
     1981 ttcttcaact gcctgtaccg cctttttgtc acgcagacga atttcaaaac ggttgataat
     2041 atcggtatgt ttctttttcg ttgcctgttc cttttgtttt tcatacaggc agaaatattt
     2101 tgtgcttgcc tttgagccga tataaagggt acttgctaaa ttacggtttt ttccgcttaa
     2161 ttttccacct tgtacatttt catagttttt acagcgacat tccgcaccac catttctgta
     2221 tttttcagat agcacaggaa tatccagcag accgcacata tcattgattg ccaagtcaaa
     2281 acgtcggata acaccgccac agtcaagaca gcggtttaag aatgaatacc agtccctgtt
     2341 ctgtgcctgt aagacatatt ccatgttgcg tgagcctgcc ccttttaatt ctaaaaatac
     2401 gcccatgtgt tcatcatcag aagccatgac cttgatttcc ccgtaagcat aatgctcctt
     2461 atagccataa tacccatagt cataatggat aaaataatct gctttcatgc caagcacctt
     2521 tcggataact tccaatgcgt cagtcgttgg aaaacggaca gacagataat caacgagcag
     2581 ataaaaaggc ttatcgcaga tatagtaatt cagacaacgc aggataatgt tctgtgtttc
     2641 cttgctggcg ttagcttttc cgttctcaaa gcggttgata ctatatcttg atacatgggt
     2701 aagacttgca agttctcctt gtgaaagacc gctggcaaga cgtttgttct tcatttcaag
     2761 tgcaaattct aaattgttca ttgtattcct ccaatcccac aatagccttg aaatacctgt
     2821 aaaccacctt tcaaaaagtg tgatatgtac tatcttctta aaacctctca ttactactca
     2881 caggtttggg ggtgtatggt ttttgacttg tcttttgtga aatagtgccc ccctgttaga
     2941 taacgggggc acagtcaagg ctcgcaggct cgccagccga caaagcattt ccgtcctttg
     3001 acggcaagcc gtcatggcgt tgttgctgac gcacgccagc agtcctacaa tgctttgtca
     3061 gccagtcctg tttttgtctt tctaacaagt tccattttaa ccaatattca tatctacggg
     3121 tttaatcaga taggtgtttc tttttgaata tttttgtatt tcattctatt gtgcttattt
     3181 aattgcaaga acatagtaat tcttatatgc ttatttgtca atatcttttt ttgcctttac
     3241 aaatcatatt aagcatgata gacttataac cgcgaagata taacgagata tggagggtta
     3301 tgcaatgaaa ataagccatt taggaaataa tatacagacc ataagaaaat ttagaggaat
     3361 gaaacaacag gaacttgcgg acaaaatcgg tatcaatatg cagagccttt ccaagattga
     3421 acgaggattg aattatcctg cttatgaaac gctggaaaag ataatggaag tgctggacgt
     3481 tacgccgaat gaattgttgt cgggggaatg gaaatatgtc aatcaatccg aaaaggaagt
     3541 ctgccagttt ttaagagcag aagaacgact aaatgcagag ctgaaacatg gacactatga
     3601 taacttcttt gacagcgagg aagaatggct ggaatatgag ttagaaaagt tacgggaata
     3661 cattaccgac tacatcaatg gaacaaacat tgaagcgtct gacctttacc taatcaaaga
     3721 atttatccag catttaaagt tccaaaaact actagaccgc tatgatgatt tatacagcat
     3781 ggatatgttt ggggaaagca tagagggaca taagtataag acaccttatc aagtcgtgaa
     3841 aatgataaac ccaaattcaa aagaagatat ggaactgtta cgagaagtat taaggaataa
     3901 ccattttgat gatgaggacg agtaatcttt ttcctcttgc aagaagatac agaaaacaaa
     3961 aagcccagta aagagtgcaa agcaccacca atggtggcaa gctcttgaca gggctttttc
     4021 ttttctgtgg ttggtaatca agaggaagaa agggataaaa cctaatttat gataaatcag
     4081 acaacacaac tagcaagttt gagcttgtgc tacacgtgcc ataaagtaac acacaaggat
     4141 tgatagatac cagcccacta tgccctaagt tttattatgg aagtaaacaa ttaacgatat
     4201 tttaacgctt gtgtattctt ttgagcgaat gttattagcg gttgacagat ttccacctct
     4261 tattggtaga atgttaaacc tcgattttgg tataattaaa ccacaataaa aaagcaaaaa
     4321 ggagtgctga atatgaattt taacgaaaaa ttgatttctt tgagaaaaac caaaggatta
     4381 tcccaagaag aactgggagc agaacgaatc aaacaatgag aaaggaagat ttaatgatga
     4441 aatttagaaa aaatcagaat aaagaaaaac agataccaaa ggaaaagaaa cctcgtgtct
     4501 ataaggtcaa tcctcataaa aaggttgtga ttgccttgtg ggtactttta gggcttagtt
     4561 tcagctttgc gatattcaag cactttacag ctatagatac tcatactatt cacgaaacaa
     4621 ctatcataga aaaggaatac gttgatactc atcatgtaga aaattttgta gagaactttg
     4681 cgaaagtcta ctattcatgg gagcaatccg ataagtccat tgataatcga atggaaagtc
     4741 taaaaggcta tctgacagag gaacttcaag ctctcaatgt tgatacagta cgcaaagata
     4801 ttcctgtatc gtcttctgta agaggatttc agatatggac ggtagagcca actggcgaca
     4861 atgagtttaa tgtaacctac agtgtagacc agctcattac agagggagaa aatacaaaga
     4921 ccgtccactc tgcttatata gtgagtgtct atgtagatgg ttctggaaat atggtactgg
     4981 ttaagaatcc gaccattacc aacataccta agaaatcaag ttataaacca aaagccattg
     5041 aaagtgaggg gacggttgat tccattacaa ccaatgaaat caatgagttt ttaacgacgt
     5101 tcttcaagct ctatcctaca gcgacagcca gtgaactttc ctactatgtg aatgacggga
     5161 tattaaaacc aatcggaaaa gagtacatct ttcaagaact ggtaaatcct attcacaatc
     5221 gtaaggataa tcaagtcacg gtatcgctga cagtggagta tatcgaccag cagaccaaag
     5281 caacgcaggt atctcaattt gatttggtac ttgaaaagaa cgggagtaat tggaagatta
     5341 tagaataaca aatattggta cattattaca gctattttgt aatcacgtac tctctttgat
     5401 aaaaaattgg agattccttt acaaatatgc tcttacgtgc tattatttaa gtatctattt
     5461 aaaaggagtt aataaatatg cggcaaggta ttcttaaata aactgtcaat ttgatagtgg
     5521 gaacaaataa ttggatgtcc ttttttagga gggcttagtt ttttgtaccc agtttaagaa
     5581 tacctttatc atgtgattct aaagtatccg gagaatatct gtatgctttg tatgcctatg
     5641 gttatgcata aaaatcccag tgataaaagt atttatcact gggattttta tgcccttttg
     5701 ggtttttgaa tggaggaaaa tcacatgaaa attattaata ttggagtttt agctcatgtt
     5761 gatgcaggaa aaactacctt aacagaaagc ttattatata acagtggagc gattacagaa
     5821 ttaggaagcg tggacaaagg tacaacgagg acggataata cgcttttaga acgtcagaga
     5881 ggaattacaa ttcagacagg aataacctct tttcagtggg aaaatacgaa ggtgaacatc
     5941 atagacacgc caggacatat ggatttctta gcagaagtat atcgttcatt atcagtttta
     6001 gatggggcaa ttctactgat ttctgcaaaa gatggcgtac aagcacaaac tcgtatatta
     6061 tttcatgcac ttaggaaaat ggggattccc acaatctttt ttatcaataa gattgaccaa
     6121 aatggaattg atttatcaac ggtttatcag gatattaaag agaaactttc tgccgaaatt
     6181 gtaatcaaac agaaggtaga actgtatcct aatatgtgtg tgacgaactt taccgaatct
     6241 gaacaatggg atacggtaat agagggaaac gatgaccttt tagagaaata tatgtccggt
     6301 aaatcattag aagcattgga actcgaacaa gaggaaagca taagatttca gaattgttct
     6361 ctgttccctc tttatcatgg aagtgcaaaa agtaatatag ggattgataa ccttatagaa
     6421 gttattacta ataaatttta ttcatcaaca catcgaggtc cgtctgaact ttgcggaatt
     6481 gttttcaaaa ttgaatatac aaaaaaaaga caacgtcttg catatatacg cctttatagt
     6541 ggagtactac atttacgaga ttcggttaga gtatcagaaa aagaaaaaat aaaagttaca
     6601 gaaatgtata cttcaataaa tggtgaatta tgtaagattg atagagctta ttctggagaa
     6661 attgttattt tgcaaaatga gtttttgaag ttaaatagtg ttcttggaga tacaaaacta
     6721 ttgccacaga gaaaaaagat tgaaaatccg caccctctac tacaaacaac tgttgaaccg
     6781 agtaaacctg aacagagaga aatgttgctt gatgcccttt tggaaatctc agatagtgat
     6841 ccgcttctac gatattacgt ggattctacg acacatgaaa ttatactttc tttcttaggg
     6901 aaagtacaaa tggaagtgat tagtgcactg ttgcaagaaa agtatcatgt ggagatagaa
     6961 ctaaaagagc ctacagtcat ttatatggag agaccgttaa aaaatgcaga atataccatt
     7021 cacatcgaag tgccgccaaa tcctttctgg gcttccattg gtttatctgt atcaccgctt
     7081 ccgttgggaa gtggaatgca gtatgagagc tcggtttctc ttggatactt aaatcaatcg
     7141 tttcaaaatg cagttatgga ggggatacgc tatggctgtg aacaaggatt gtatggttgg
     7201 aatgtgacgg actgtaaaat ctgttttaag tatggcttat actatagccc tgttagtacc
     7261 ccagcagatt ttcggatgct tgctcctatt gtattggaac aagtcttaaa aaaagctgga
     7321 acagaattgt tagagccata tcttagtttt aaaatttatg cgccacagga atatctttca
     7381 cgagcataca acgatgctcc taaatattgt gcgaacatcg tagacactca attgaaaaat
     7441 aatgaggtca ttcttagtgg agaaatccct gctcggtgta ttcaagaata tcgtagtgat
     7501 ttaactttct ttacaaatgg acgtagtgtt tgtttaacag agttaaaagg gtaccatgtt
     7561 actaccggtg aacctgtttg ccagccccgt cgtccaaata gtcggataga taaagtacga
     7621 tatatgttca ataaaataac ttagtgtatt ttatgttgtt atataaatat ggtttcttgt
     7681 taaataagat gaaatatttt ttaataaaga tttgaattaa agtgtaaagg aggagatagt
     7741 tattataaac tacaagtgga tattgtgtcc tgtatgtgga aataaaacac gattaaagat
     7801 aagggaagat actgaattaa aaaaattccc cctctattgt ccgaaatgca gacaagaaaa
     7861 tttaattgaa ataaagcagt tcaaagtaac tgtgattaca gagccagacg caaagacgca
     7921 gagccgataa aatgagatta atacaatctc attttatcgg ctctttccgt tatgtatgga
     7981 ttcttttaat tagtcttcga tgtttcttgc ttcgttgata ccgctggcta aagattccat
     8041 taaggatagt tctttgtctg taaagctatc catgtatttc tctatctgta atcgtcgggt
     8101 gctttttacc aagttattag caggtaagaa aaattcatca acggaaacat gaagtaacga
     8161 tacaaggtca taaagaactt gtatgctggg gtgttgccct ttattttcaa tattagttaa
     8221 gtaccgtggg tcaatttcaa tcaatgctcc cacttgttca cgagttaaac ctcgtttcaa
     8281 tcgagcttct ttaatggcta aaccaaaggc tctaaaatca tatttatctt cttttttacg
     8341 catagtagac cacctctata cattttattg ttcctactga attaaaaaca ggtatagaaa
     8401 aacgtgttat atggtttata ggtttatatt taataaaaag cactactaaa cgccaataaa
     8461 aaaaaccgtt atatggtagt gctatttacg ctgttaaaat attgtatatt acttccaaat
     8521 ggcggtttgt tggaggtcaa cgtcgccatg aagtacatca tatacaataa atttccttac
     8581 attgggttct tgtcaaaaaa agtcgtctat ctgcaataga taagtacgtc caccaatgtg
     8641 gttttataaa tcatatagat agaataacag aagcatgtaa acagagaaat aaatctgttt
     8701 atatgctttt ttggctattc agaacttttt tacaaagttt atttatcagt aatgcaacaa
     8761 atcccccttt cacattggga ctaagagtga aaggagataa acgagcaagg ctcacttcct
     8821 ttcctagaca gaaagggggt gagaaacatg aaaccatctt cttttcagac cacaatagaa
     8881 aatcagtttg actatatctg taaacgtgct atggaagacg agcgaaagaa ttatatgctt
     8941 tatctttcaa ggattgcaaa gcgtgaggtg tccttttcgg atgttggcga ttatcttgtt
     9001 agccagtttg cgacaacaga taactattcc tgtgattgca ttttttttaa agtcttgaat
     9061 tatacaatcc aagtatcaaa tgagaaactt gctgttgctt tatataagtt atcgagtaag
     9121 gagcgtgatg taattctatt acgctatttc caaagcatga gcgaccaaga aatcgcagaa
     9181 ttataccatg tatcacgttc tgctatttat cgcagaagaa gtaatggatt gaagaaatta
     9241 aaaatagtat tgaaagaaag gaattgaacc aatgaaaaaa agaatatggt taccctacat
     9301 taaaagtaat atgtcaagcg tctgctggaa gcgaaactgc cataagagag attttaaaat
     9361 tttatgacgc ttatatttgc aagttatgct tgcgtccgtt ttaccactct gaaagtggta
     9421 aaatcactat gcaggttgat gaagaactaa aaggtcaaat tcatacagaa atgatgaaag
     9481 caatcttgaa atttgaaatc agagtgaagt aatcatatta catgaatgaa aaacgggaga
     9541 tacacagatg tttgtgctat ctcccatgtt attttttgtg tcatatcaag gctcattcaa
     9601 tcgtggtaaa ttcgtggtaa aatgagtgtt tttctatact tcaaaatgtt tgaaagtata
     9661 gtgttcatgc ggataatcaa aaatcggata taaaattt
//
LOCUS       DAGSRM010000225         7103 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT106 isolate 14.7920105
            SAMN25516843-rid13958643.guided.141, whole genome shotgun sequence.
ACCESSION   DAGSRM010000225 DAGSRM010000000
VERSION     DAGSRM010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516843
            Sequence Read Archive: SRR17631130
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250290.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250290.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 03:28:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,733
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,613
            CDSs (with protein)               :: 3,613
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 22 of 46
            Pseudo Genes (incomplete)         :: 18 of 46
            Pseudo Genes (internal stop)      :: 17 of 46
            Pseudo Genes (multiple problems)  :: 10 of 46
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7103
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516843-rid13958643.guided.141"
                     /strain="RT106"
                     /isolate="14.7920105"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2014-01-13"
                     /collected_by="ModMedMicOxford"
     gene            68..475
                     /locus_tag="MJE33_003725"
     CDS             68..475
                     /locus_tag="MJE33_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBY9649592.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFLNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMQEH"
     gene            477..707
                     /locus_tag="MJE33_003726"
     CDS             477..707
                     /locus_tag="MJE33_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBY9649593.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINVILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            complement(759..968)
                     /locus_tag="MJE33_003727"
     CDS             complement(759..968)
                     /locus_tag="MJE33_003727"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBY9649594.1"
                     /translation="MVQVTAKQPFQRLFTNHYIVALLIKVMAYSICWLSVSQTYLSTL
                     FSCQRTVGIDLPIKNHNSLWAFIDK"
     gene            1097..1300
                     /locus_tag="MJE33_003728"
                     /pseudo
     CDS             1097..1300
                     /locus_tag="MJE33_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
     gene            1378..1731
                     /locus_tag="MJE33_003729"
     CDS             1378..1731
                     /locus_tag="MJE33_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
                     /protein_id="HBY9649595.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVYSFGKAQFVYSWK
                     LVPTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLYAKKNVLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            1803..2435
                     /locus_tag="MJE33_003730"
     CDS             1803..2435
                     /locus_tag="MJE33_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBY9649596.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
     gene            complement(2806..3675)
                     /locus_tag="MJE33_003731"
     CDS             complement(2806..3675)
                     /locus_tag="MJE33_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBY9649597.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
     gene            complement(3772..4629)
                     /locus_tag="MJE33_003732"
     CDS             complement(3772..4629)
                     /locus_tag="MJE33_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBY9649598.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            complement(4843..6861)
                     /locus_tag="MJE33_003733"
     CDS             complement(4843..6861)
                     /locus_tag="MJE33_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBY9649599.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYVFSYDLK
                     RAVYVPFGLMYIFMVSIPVGKSEFPMRLSALAVGAVIIMIAQFVMNRNRMKNVGDKEL
                     ISICDELLEKISLLKNTINDDSSIIKSSMRKIDSCNYRINSISKNLKMVIFDNRKDDF
                     YISIRGIDIMNILFSLERISLILERYKKDSKEFEDEDIKNILEESSINSKSDVLVVAT
                     KEINYIKMCLENKDTITDKEILGFRDYVISQDTKNINLKEIYSVLENLYEFLVDYKKV
                     KSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHLR
                     DGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLLA
                     GYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFILP
                     YDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNLLLIPSLIEDRLMLINAI
                     YKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKILRDTNYISNYNVDKYDEG
                     RSVILESIVNTKSLGDKIICLNLLQTLNGVKEMYRISNITKVSIKEAA"
BASE COUNT         2477 a         1107 c          821 g         2698 t
ORIGIN      
        1 ccctcccacc gtgataggag taagcaaggt tatttaactc cttgcgatgg aaagagggtg
       61 aaatcttatg gttgatgaaa agacaattga acatcaattc gacagtttct gtaaaacagt
      121 tttgcgtaac tatgctagag atatttatga cgaaaataaa cgcagaaatg attatttggt
      181 atctctcgaa tctttgtcac ttgcagaatt aagtaaattg agtattttag atgattatga
      241 ttctaactac atctgtatgg tttcttacga ctacaacata cgaattgaag atgtattaat
      301 ggcacaagca atcggaaaac ttacaaaaag aaaacaagat ataattctac tttcattctt
      361 tctcaacatg acaaatgctg acatagcaac attgatggat ttagcagaaa ataccataca
      421 ttatcacaaa acaaatgcac taaaagagtt gaaagaactc atgcaggaac attgaaatga
      481 aacctactaa agaagtatta ccttttacaa ttattttact tgctgtacat ggcgatgtaa
      541 atgcaataaa cgtaatactt aaacattttg aacactatat cattcgtctt tcacagaaaa
      601 ctttatttga tgaatacgga aatccctata ttcatgtaga agaagaaaca aagcgaatgc
      661 taggaacaaa actaatcact gctattttga gatttaattt gtcttaatct cgccctctct
      721 ccatgtatca agctatttgg tacatggaga atcactaatt atttgtctat aaaagcccat
      781 aagctattgt gattttttat aggcaaatca atgccgactg ttctttgaca actgaataaa
      841 gtagatagat acgtttgtga tacagatagc caacaaatac tatacgccat gaccttaatc
      901 aagagagcta ctatatagtg atttgtgaat aacctttgga acggttgttt tgccgtaacc
      961 tgtaccacag tataatgata cacctgtatg acacggttct gcccataaga atgggattgg
     1021 ttgaaatacc aatggagctt accaaagcca tctatctact tctgaactac gaacatagaa
     1081 aggagtgata cattctatga acaatactga tgtacctatt tgggaaaaat ataccttgac
     1141 gattgaataa gcgtcaaaat actttcgtat tggagagaaa aaattacgta aacttgctga
     1201 tgaaaacctt gattctggct gggtcattat gaatagcaat cgcattcaaa taaagagaaa
     1261 acaatttgag aaaatcgttg atacacttga cgttatctaa tgaaattgag ccttatatgt
     1321 tataatattc atatgacgta tcaagctcaa ttcggttatt gagaggagct tcttatcatg
     1381 tcaactgaaa aaagacgaga taataaagat agaattttaa gaactggaga gagccagaga
     1441 aaagatggaa gatacgcata taagtatgta tattcatttg gtaaagcaca atttgtttac
     1501 tcttggaaac ttgtacctac tgataaaccc ccaaagggaa aacgtgatga tatatcatta
     1561 agggataagg aaaaagcaat acaaaatgac cttgatgata gcattgacac tattggtaag
     1621 aaaatgacag tttgtcagtt atacgctaaa aagaatgtac taagaaagaa tatccgactt
     1681 aacactaaaa aaggacgcca ttatcttatg aagattctta acaaggagtg attctgtgaa
     1741 gagatgtatt gtagtagtgg aatagtcaaa aacataatat atattaaagg agagatttaa
     1801 atatgactat tccaagttca aataaggtat atccaagaag taatgattat caaactgtat
     1861 atcttaaaaa tgtaataaca agggaaaata tagaagttgg agattacaca atatataatg
     1921 acttttataa tgacccaaga gattttgaga aaaacaatgt attgtatcat taccctataa
     1981 acaatgataa attaataatt ggtaagtttt gctcaatagc atgtaatgca aaatttctaa
     2041 tgactagtgg aaatcactca atgaaatcaa aatccacata tacttttccc atcttttatg
     2101 aagaatggaa actcgatatc aaccatataa cggacgcttg ggacaataag ggtaacatta
     2161 taattggaaa tgatgtgtgg ataggttatg attctataat tatgtcaggt gtaaatattg
     2221 gtgatggtgc aattattgga acaagagcag tagttactaa tgacgttcca ccgtattcta
     2281 ttgttggagg agtacctgca aaaattataa aaaagagatt taataatgat acaatttcaa
     2341 aattattaga aattaaatgg tgggattggc catataaaaa aattcaatca aacattaagc
     2401 atattcaatc aggaaatatt gataaattag tttaatattt tataatttat ttatattaaa
     2461 ctttacttta ataatcattt tataagagct ttttcaaaat ttgtatatta ttttaatata
     2521 tataaattta atacaacatt aaataaaata aaaagtaaag ttacttaata aaaagttact
     2581 ttacttttta ttatttaaat tcctgttttt attataatag gtaaaattct attaaactag
     2641 ttcacatttt cattattatt gatatattat atatgaatat accattttgt tttttaataa
     2701 aaaaatcatg ctacatcaat agtaagcaac tattaatgta gcatgatttt ttgtatctaa
     2761 ataatcttag tatttaccta tcaatataga ttgatatatc aaaaactata gtcttctata
     2821 ttcttctctt tttagctttt ggttacttag atagtaatca atttcatcaa ttagacgttg
     2881 tttatcatca ggtaatacac cttttaaatt agcataattt gatgcatgat tacttctaaa
     2941 tacaattttt tcattagcat ctatattctt tatcatctct ttaatttctt gtaacacagc
     3001 cttatcctct agtattgtaa actctcctgc cttaagtaaa tcgtaaagtt cagtattagg
     3061 ttctaccatt aaactaagta cacccacata atctggtgaa attgctccta acatttttcc
     3121 tgtatcaact gcatgctgat gtgttttttt agttcctcca agacctgcta ttacagtggc
     3181 tgataattta atcccacttt ttttaacttt tctaccaact tttattaatt gttcactact
     3241 aaaacctttc ttaatatctt ctaatacttc attgcttcca ctttcaagcc ctagataaat
     3301 cattttaaca ccaagttttc ttaattcttt aagttcatca tcagattttt tctctataga
     3361 tttaggagag ccgtacatag taattctttc acactctgga aaaacttctt ttatatataa
     3421 aataatatct cttaatttct ccattggtat tatcaatgca tctccatctg ctaagaatat
     3481 tctttctaca tgcttataat atcttctaaa tatatcaatt tcatttttta ttacttctag
     3541 tggctttatt ttaaattttt tagatttgta catactacaa aacgtacatt tgttatgaga
     3601 acatcctagt gtgacttgta ttataagact ataagcctca cttggtggtc tgtaaagtgg
     3661 catatcgtat tccatattaa aatccccctt gtaataaaat attcattcta ttatattatt
     3721 aatatttact ttaaataata acttataaaa ttatattttt taaattttag tttaagatat
     3781 attctttaaa aatttaatga tactattggc agtaatatta agttgttttt cttgacttat
     3841 aatagcatca ttatctccct tttgttttcc ataatctcca aattgtgcat gatttcctcc
     3901 ttcaatctca acataagttg tatcatcagg aagtttttgt tttgattcta caagactttt
     3961 aaaattgact actccatctt tgctccccca tatagatata actttttttc ctaattcttt
     4021 caactcatca cccattggat aagaagctaa aaatacaacg ccttcaatta atttattatc
     4081 acgtgcaaaa tttgatgctg caactcctcc taatgaatga cctcctatta cccaatgttc
     4141 tatgttgttg taagaatcca taactttctg tgccttatta gttccaagta tagctagatt
     4201 aagtggcatc tttacgatta caacctcata tccactttct gctatttttc ttgcaagtgg
     4261 agcataagat tctatttcta ctttagctcc tggatacaaa attaaacctt tagaaacttt
     4321 tcttcctttt ggtgtaaatg aaatgttttc atttacagtt acttcaactt tactattgct
     4381 gactagagct tcttttgcca gcttttgagg ttcatatgtt ttatttatcc atatgaaaaa
     4441 gccaagcaaa atcaatacaa atagtatacc tattccctta aatatcttat tctttatgtt
     4501 aaattttcta ttattatttt tatcatcttt gttctcaacc tcatcataaa gtaaaggtct
     4561 ctcaagttcc atagttatac taaatgtatc atatttctta ttacgcttta cctttacatc
     4621 tttactcata aatctaaccc ccataatatt tgaatactat tatagtttat cctaagtaaa
     4681 ttgttttttc aaccaatgaa aaaaatcaat attttaacta ttttcatata aaattcataa
     4741 atatatgttt ttgtttttta tatattattt tcttttagta ttttataaaa caaatagcca
     4801 tatactttat tttaaatata tggctattct ataatttaat atctaagctg cttcttttat
     4861 tgaaacttta gttatattac ttattctata catttctttg acaccattta gagtttgcag
     4921 taagtttaga catattatct tgtcacctag acttttagta tttacaatac tctctaaaat
     4981 tacacttctt ccttcatcat acttatctac attgtaattt gaaatataat tggtatctct
     5041 caatattttt tcaacatctt catcttttat tttattttta ttaacattta tatatagatt
     5101 atacatatta cttattagta atttctgatt ttctaaaaaa tcctcttgat gtttatcttt
     5161 atatatagca tttatcaaca taagtctatc ttcaataagt gatggtatca ataataaatt
     5221 ttttatatag taaacatcag ctacattttc tatagactta ttgacttcat caattatatt
     5281 tttaactatt cctttataca tttctataac atgttgatag cctgttttag catcatatgg
     5341 taatataaac ttatttacta ttaatgcaat aattgctcct aatgctacat atgaaatcct
     5401 gcttattgtt aaaacatttg ggtctcccat aactactgct gaaccaagag ctgaaacagt
     5461 tacacatacc attaattttc tataatcaac tacataagag tttatatacc ctgctaaaag
     5521 aactattaac gaccttaatg tactatcttt tattattgaa aataatacaa taaatattac
     5581 agctcctata aatgtacctt ctattctttt tctagacctt tgtatacaat tttctgcgta
     5641 aggttgtgtt aatgaaaaga cagttaacat tatccatctt ccatctctta aatggaaata
     5701 atccataata aaacccgcta cagctgtagc taaagcaatc tttactgcat aagaaaatct
     5761 tatagaattt aaattaaagt ttttcttata tattgatagt cgtttaaatt catgaggtat
     5821 ctttgatttt ctttcagctt tcttttcgtc tcttgatttt acttttttat aatctactaa
     5881 aaactcatat aaattttcta aaacactata aatttctttt aaatttatat ttttagtatc
     5941 ctgagaaata acataatctc taaatcctaa tatctcttta tcagttatgg tatctttatt
     6001 ttctaaacac atcttaatat agtttatttc tttcgttgct acaactaaaa catctgattt
     6061 actatttatg ctactttctt ctaaaatatt ttttatatct tcatcttcaa attctttact
     6121 atcctttttg tatctttcca ggattaaact tattctttct aatgaaaata aaatattcat
     6181 tatgtctata cctctaatac tgatataaaa gtcatctttt ctattatcaa aaataaccat
     6241 tttcaaattt ttagatatag aatttattct ataattacag ctatctattt ttctcatact
     6301 agatttaatt atagaactat catcattaat agtatttttt aaaagactaa ttttttctaa
     6361 taattcatca catatagata tcagttcttt atctccgaca tttttcatcc tatttctatt
     6421 cataacaaat tgagcaatca ttattataac tgctccaaca gctaaagccg acaatctcat
     6481 aggaaattca ctttttccta caggtatact taccataaat atatacatta gaccaaaggg
     6541 tacatatact gctcttttta agtcataact aaatacatac cctattataa ataatgctat
     6601 aaaatttaat aaaactccta aatacatatt ttgtactgca aaaaatgata gtattcctaa
     6661 gattatattt attgctaaat acttcagtaa atttttaatt ggagaaatag ttaaatctct
     6721 ttccaagagt gttagcatag ctgttactat acctacacct ataagtgtat ttttacttcc
     6781 aaataaatat ataaatatag aaataaatgc tattataaaa ataaacaact ttgtttttga
     6841 tattactagt ttctttgtca tcatatcccc cctaaaaatt tcaacaagac caattttatc
     6901 atatttgtta tcttattcaa aatgcgcttt tttacggtag gactttaact ttttttatat
     6961 ttagaactat ataattcata tataaacgta ttttttatat atataaccga tatattttcg
     7021 tatgctccca tatttttttt gtttcaaata ttttttcaaa aatataaaaa taactctctc
     7081 aacaatacta aaaatgtatt tat
//
